bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of recombinant tagged equilibrative nucleoside transporter 1 (ENT1) expressed inE. coliThis paper is one of a selection of papers published in a Special Issue entitled CSBMCB 53rd Annual Meeting — Membrane Proteins in Health and Disease, and has undergone the Journal’s usual peer review process.
Authors: Reyes German , Nivillac Nicole M.I. , Chalsev Maria , Coe Imogen R. 
Published: March 2011

Nucleoside transporters (NTs) are integral membrane proteins necessary for the cellular entry of nucleoside analog drugs used in chemotherapeutic treatment of conditions such as cancer and viral or parasitic infections. NTs are also the targets of certain drugs used in the treatment of various cardiovascular conditions. Because of the importance of NTs in drug uptake, determination of the three-dimensional structure of these proteins, particularly hENT1, has the potential to improve these treatments through structure-based design of more specifically targeted and transported drugs. In this paper, we use NMR spectroscopy to investigate the structure of the large intracellular loop between transmembrane domains 6 and 7 and we also describe a method for the successful overexpression of full-length hENT1 in a bacterial system. Recombinant tandem histidine-affinity (HAT) and 3×FLAG tagged hENT1 was overexpressed in E. coli, affinity purified, and functionally characterized by nitrobenzylthioinosine (NBTI) binding. Anti-3×FLAG immunodetection confirmed the expression of N-HAT-3×FLAG-hENT1, while increased NBTI binding (3.2-fold compared with controls) confirmed the conformational integrity of the recombinant hENT1 within the bacterial inner membrane. Yields of recombinant hENT1 using this approach were ∼15 µg/L of bacterial culture and this approach provides a basis for large-scale production of protein for a variety of purposes.
Episodes of Injuries and Frequent Usage of Traditional Chinese Medicine for Taiwanese Elite Wrestling Athletes
Authors: Lin Zen-Pin , Chen Yi-Hung , Chia Fan , Wu Huey-June , Lan Lawrence W. , Lin Jaung-Geng 
Published: March 2011

Wrestling normally places extreme demands on the body and thus may cause various kinds of injuries. An in-depth understanding of the episodes of injured sites, types, timings, and treatment modalities would help participants be aware of wrestling-related injury occurrences so as to develop effective preventive measures. Therefore, this study aims to investigate the gender-specific injuries among elite wrestling athletes.Subjects were selected from the 2009 Taiwanese National Wrestling Sport Championship. Participants were adolescent wrestling athletes, ages 16–18, who must have received at least one bronze medal at national level tournaments in 2008. A total of 118 respondents, 96 males and 22 females, completed and returned the questionnaire in which demographic data and information about the types, sites, and timings of injuries suffered and treatment modalities adopted were elicited. The data were analyzed with independent t-tests.The questionnaire results revealed a significantly higher injury rate for males than for females. The top three injured sites for males were waist (11.1%), ankle joint (10.1%) and finger (9.6%); while for females were ankle joint (13.6%), knee (12.5%) and waist (11.3%). Contusions were the most frequent type of injury: for males (73.5%) and for females (70.6%); followed by tendon inflammation for males (10.7%) and accumulated injuries for females (15.2%). During training and matching periods, the frequency of injuries for males (69.0%) is lower than that for females (81.8%). Traditional Chinese medicine (TCM) with acupuncture and moxibustion was the most common treatment modalities used for males (51.8%) and for females (68.0%); followed by orthopedics: for males (29.5%) and for females (18.0%).The present study contributed as the first effort to reveal the potency of using TCM with acupuncture and moxibustion in wrestling competitions. To prevent possible brain and body injuries in wrestling, safety education, skills and rules, and scoring systems may require further revision. Increased training of wrestling health professionals and advanced research and development of auxiliary training devices and protective equipment for wrestling athletes are also recommended.
Regional spatial variations in rockslide distribution from structural geology ranking: an example from Storfjorden, western Norway
Authors: Henderson Iain H. C. , Saintot Aline 
Published: March 2011

Abstract

Structural geology has recently become a key topic in landslide research. However, the link between regional structures, their cumulative contribution to rockslide development and their significance in a spatial framework is uncertain. We examine the influence of structures on rockslide susceptibility in the Storfjorden area, a 900 km
2
fjord complex in western Norway that includes the monitored rockslide sites of Åknes and Heggursaksla. We have newly identified 52 potential rockslide sites from aerial photographs. The structural features critical for the development of large rockslides (fjord-dipping foliation, basal shear plane and breccia, extensional fracture and transfer fault) have a spatial bias in orientation. Rockslides are more likely to develop in specific fjord orientations that have favourably oriented structures. Therefore, the development of rockslides has a marked spatial distribution that we describe qualitatively with an inventory of structural features. Sites with the full plethora of features display the most movement, the largest volumes and are already under the closest scrutiny with regard to monitoring. These sites are also spatially biased, the largest clustering occurring in west Sunnylyvsfjorden. The utilization of structural criteria can show trends in spatial distribution of rockslide potential and on a regional scale can be an important tool in susceptibility analysis.
Structural analysis of Turtle Mountain: origin and influence of fractures in the development of rock slope failures
Authors: Pedrazzini Andrea , Jaboyedoff Michel , Froese Corey R. , Langenberg C. Willem , Moreno Francisco 
Published: March 2011

Abstract
Large slope failures in fractured rocks are often controlled by the combination of pre-existing tectonic fracturing and brittle failure propagation in the intact rock mass during the pre-failure phase. This study focuses on the influence of fold-related fractures and of post-folding fractures on slope instabilities with emphasis on Turtle Mountain, located in SW Alberta (Canada). The structural features of Turtle Mountain, especially to the south of the 1903 Frank Slide, were investigated using a high-resolution digital elevation model combined with a detailed field survey. These investigations allowed the identification of six main discontinuity sets influencing the slope instability and surface morphology. According to the different deformation phases affecting the area, the potential origin of the detected fractures was assessed. Three discontinuity sets are correlated with the folding phase and the others with post-folding movements. In order to characterize the rock mass quality in the different portions of the Turtle Mountain anticline, the geological strength index (GSI) has been estimated. The GSI results show a decrease in rock mass quality approaching the fold hinge area due to higher fracture persistence and higher weathering. These observations allow us to propose a model for the potential failure mechanisms related to fold structures.
Intensified Adjuvant IFADIC Chemotherapy for Adult Soft Tissue Sarcoma: A Prospective Randomized Feasibility Trial
Authors: Brodowicz Thomas , Schwameis Eva , Widder Joachim , Amann Gabriele , Wiltschke Christoph , Dominkus Martin , Windhager Reinhard , Ritschl Peter , Pötter Richard , Kotz Rainer , Zielinski Christoph C. 
Published: March 2011

Purpose. The present prospective randomized adjuvant trial was
carried out to compare the toxicity, feasibility and efficacy of augmented chemotherapy
added to hyperfractionated accelerated radiotherapy after wide or marginal resection of grade
2 and grade 3 soft tissue sarcoma (STS).Patients and methods. Fifty‐nine patients underwent primary surgery
by wide or marginal excision and were subsequently randomized to receive radiotherapy
alone or under the addition of six courses of ifosfamide (1500 mg/m2
, days 1–4), dacarbazine
(DTIC) (200 mg/m2
, days 1–4) and doxorubicin (25 mg/m2
, days 1–2) administered in
14‐day‐intervals supported by granulocyte‐colony stimulating factor (30 × 106
IU/day, s.c.)
on days 5–13. According to the randomization protocol, 28 patients received radiotherapy
only, whereas 31 patients were treated with additional chemotherapy.Results. The relative ifosfamide–doxorubicin–DTIC (IFADIC) dose
intensity achieved was 93%. After a mean observation period of 41±19.7 months
(range, 8.1–84 months), 16 patients (57%) in the control group versus 24 patients (77%) in the
chemotherapy group were free of disease (p>0.05).Within the control group, tumor relapses
occurred in 12 patients (43%;six patients with distant metastases, two with local relapse,
four with both) versus seven patients (23%; five patients with distant metastases, one with
local recurrence, one with both) from the chemotherapy group. Relapse‐free survival (RFS)
(p=0.1), time to local failure (TLF) (p=0.09), time to distant failure (TDF) (p=0.17) as well as
overall survival (OS) (p=0.4) did not differ significantly between the two treatment groups.
Treatment‐related toxicity was generally mild in both treatment arms.Conclusion. We conclude that the safety profile of intensified IFADIC
added to radiotherapy was manageable and tolerable in the current setting. Inclusion
of intensified IFADIC was not translated into a significant benefit concerning OS, RFS,
TLF andTDF as compared with radiotherapy only, although a potential benefit of
chemotherapy for grade 3 STS patients needs to be validated in prospective randomized
trials including larger patient numbers.
Retroperitoneal Leiomyosarcoma Associated with an Elevated β‐HCGSerum Level Mimicking Extragonadal Germ Cell Tumor
Authors: Froehner Michael , Gaertner Hans-Juergen , Manseck Andreas , Oehlschlaeger Sven , Wirth Manfred P. 
Published: March 2011

Patient. A 65‐year‐old man was admitted with a large primary retroperitoneal
tumor and an increased β‐human chorionic gonadotropin (β‐HCG) serum level. A germ
cell tumor was suspected; however, a computed tomography‐guided biopsy failed to enable
tumor classification. After two courses of chemotherapy, the β‐HCG serum level had returned
to the normal level and a diagnostic laparotomy with incisional biopsy was performed.
The immunohistochemical examination of the specimen identified the tumor as a
retroperitoneal pleomorphic leiomyosarcoma.Discussion. Tumor markers play only a marginal role in the work‐up
of patients with soft tissue sarcomas. In men with suspected retroperitoneal sarcomas,
however, the determination of germ cell tumor markers occasionally enables a preoperative
distinguishing of primary retroperitoneal germ cell tumors with considerable consequences
for management. In this setting, a retroperitoneal tumor associated with a moderately
elevated β‐HCG is a diagnostic dilemma, and surgeons should be aware of the pitfall
of a β‐HCG‐producing leiomyosarcoma in the differential diagnosis.
Pancreatic Head Mass from Metastatic MeningealHemangiopericytoma
Authors: Teh Bin S. , Lu Hsin H. , Jhala Darshana N. , Shahab Imran , Lynch G. Rush 
Published: March 2011

Purpose. To illustrate the propensity of meningeal hemangiopericytoma
to spread extraneurally, as a distinction to the ordinary meningioma.Patients or subjects. A patient with long history of meningeal
hemangiopericytoma was reported.Methods. A case report on meningeal hemangiopericytoma with a literature
review was presented.Results. The patient has multiple local recurrence as well as distant
metastases.This is the first case report of metastatic meningeal hemangiopericytoma causing
compression of the pancreatic head.The patient also has biopsy‐proven pulmonary
metastases.The patient received both local and systemic therapy.Discussion. It is important to recognize the distinctive features
differentiating meningeal hemangiopericytoma from meningioma. The positive impact of
clinico‐pathological correlation on patient management is emphasized.
Confirmed Angiosarcoma: Prognostic Factors and Outcome in 50Prospectively Followed Patients
Authors: Espat N. J. , Lewis J. J. , Woodruff J. M. , Antonescu C. , Xia J. , Leung D. , Brennan M. F. 
Published: March 2011

Purpose. Angiosarcoma is a rare tumor with endothelial cell
differentiation that may arise in any anatomic location.The purpose of this report was to
identify prognostic factors on outcome in a group of prospectively followed patients with
confirmed angiosarcoma.Subjects. Adult patients (>16 years old) with angiosarcoma treated
between July 1982 and February 1998 were identified from a prospective database.Methods. Pathologic confirmation of all cases was performed prior to
inclusion in this analysis. Various prognostic factors were evaluated for disease‐specific
survival. Survival was determined by the Kaplan– Meier method. Statistical significance
was evaluated by log‐rank test for univariate analysis and Cox stepwise regression for
multivariate analysis (p<0.05).Results. Fifty patients were identified; at the initial evaluation, this
group included 32 patients with a primary tumor, three with local recurrence and 15 with
metastatic disease. Tumor sites included 16 head and neck and skin of head, eight
extremity, seven trunk, six breast, five pelvis, four viscera and four thoracic. Median
follow‐up among survivors was 71 months (range, 38–191 months).Two‐ and 5‐year
disease‐specific survival was 50 and 30%, respectively, with a median of 24 months.
The factor predictive of tumor‐related mortality was presentation status
(p=0.001; relative risk, 5). Two‐year disease‐specific survival for patients presenting
with recurrent or metastatic disease was 13%, compared with 70% for those
with primary disease.
Chondrosarcoma of the Pelvis: Oncologic and Functional Outcome
Authors: Wirbel Reiner J. , Schulte Michael , Maier Bernd , Koschnik Martin , Mutschler Wolf E. 
Published: March 2011

Purpose. Chondrosarcoma (CS) most commonly involves the pelvis.
The factors that influence local and systemic control of pelvic CS and the functional outcome
should be evaluated.Patients. Fifty‐one patients (37 males and 14 females; mean age, 39.4 years)
with pelvic CS were included in this retrospective study.Methods. The tumor stage, surgical treatment, surgical margin achieved,
complications, incidence of local recurrence (LR), incidence of distant metastases, and the
oncologic and functional status were evaluated. Oncologic outcome was estimated
by the method of Kaplan and Meier, and the functional status was scored according to
Musculoskeleral Tumor Society (MSTS) criteria. Analysis of variance was used to determine
the factors that influence the oncologic and functional outcome.Results. Surgical stages were IA in three cases, IB in 23, IIB in 23, and III
in two. Hemipelvectomy (H) was performed in 13 cases, internal hemipelvectomy (IH) with
endoprosthetic replacement in 17, and continuity resection (CR) in 23.Two patients received
IH and CR, one due to LR, and one due to instability. Radical or wide margins were achieved
in 27 cases, marginal margins in 16, and intralesional margins in eight. Local complication
required additional surgery in 10 cases due to local infections and/or hematomas.Two
patients died perioperatively. In 48 out of the 49 remaining patients, follow‐up
was available with a mean duration of 73.4 months (range, 4–229 months).Twenty patients
died of the disease, two patients are alive with metastases, four patients are disease free
after LR, and 22 patients show no evidence of the disease. LR occurred in 10 cases (20.4%),
and 17 patients (34.6%) developed distant metastases. Functional evaluation of the 28
survivors revealed good and excellent results in 19 cases, fair in three and poor in six.The
mean MSTS score of all survivors was 69.2%, after H it was 37.6%, after IH was 61.4%, and
after CR was 79.5%.Conclusion. In pelvic chondrosarcoma, survival was determined by the
tumor stage and the surgical margin achieved.The incidence of LR was influenced by the
surgical margin achieved, whereas the incidence of distant metastases was influenced
by the tumor stage. The best oncologic results in chondrosarcoma involving the innominate
bone could be found in low‐grade tumors, and the best functional results after continuity
resection and restoration of the pelvic girdle.
Recurrent Episodic Foot‐Drop Following Surgery to the Thigh
Authors: Maiya Srinivas , Tan Simon , Grimer Robert J. 
Published: March 2011

Subject. We present the case history of a 47‐year‐old lady who,
10 months following excision of a soft tissue sarcoma from the left thigh, was struck with
recurrent episodes of foot drop.Discussion. The curious phenomenon of recurrent foot drop was found
to be secondary to pressure symptoms from a tense seroma of the thigh. She underwent
surgical excision of the sac and had immediate and complete relief of symptoms.
A Case of Ovarian Fibromatosis and Massive Ovarian Oedema Associated With Intra‐Abdominal Fibromatosis, Sclerosing Peritonitis and Meig’s Syndrome
Authors: Spurrell Emma L. , Yeo Yen C. , Rollason Terence P. , Judson Ian R. 
Published: March 2011

Purpose:To discuss a case of ovarian fibromatosis/massive ovarian oedema, intra‐abdominal fibromatosis, sclerosing
peritonitis and Meig’s syndrome. To review the reported therapeutic options.Patients: Case report of a 27‐year‐old female with the combined pathology of ovarian fibromatosis/massive ovarian oedema,
intra‐abdominal fibromatosis, sclerosing peritonitis and Meig’s syndrome.Methods: This patient was treated with supportive care and cytotoxic chemotherapy.Results: Despite the benign nature of the ovarian pathology, this patient presented with life‐threatening complications.
Response to treatment was probably multi‐factorial combining the effects of cytotoxics, use of steroids and good supportive
care. She remains in complete remission 4 years post completion of chemotherapy.Conclusion: There are reports in the literature of ovarian fibromatosis/massive ovarian oedema, luteinised thecomas, intraabdominal
fibromatosis and Meig’s syndrome occurring together in a variety of combinations. Treatment has been described
with radiotherapy, cytotoxic and non‐cytotoxic chemotherapy regimens. This case provides a link between ovarian
fibromatosis/massive ovarian oedema, intra‐abdominal fibromatosis, sclerosing peritonitis and Meig’s syndrome not
previously described.
High‐Dose Chemotherapy Followed by Peripheral and/or Bone Marrow Stem Cell Transplant in Patients With Advanced Sarcoma: Experience of a Canadian Centre
Authors: Hotte Sébastien J. , Smith Anne M. , Bramwell Vivien H.C. , Howson-Jan Kang 
Published: March 2011

Purpose: Few reports have been published on the evaluation of stem cell auto transplantation for chemosensitive sarcomas.
Some suggest benefit, others do not. We present results of 24 patients with sarcoma undergoing autotransplantation at a
Canadian institution.Patients and Methods: Twenty‐four patients were treated between 1988 and 1998: 23 were ≥18 years (median 27; range 12–56); genders were equal; 12 patients had Ewing′s sarcoma. At diagnosis, 12 (50%) had metastatic disease. Prior to
autotransplant, all had ≥1 chemotherapy regimen. Fourteen (58%) were in complete remission (CR) and seven (29%) had minimal residual disease. All received etoposide 60 mg/kg (Day –4), melphalan 140 mg/m2 on (Day –3) and a stem cell
reinfusion (Day 0).Results: Three patients (12.5%) were alive and disease‐free with median follow‐up of 92 months (80–142); one was alive
with disease 32 months post‐autotransplant. Twenty had died (disease, 17; transplant‐related, 2; unknown, 1). Of the four alive, three had Ewing′s sarcoma, one alveolar rhabdomyosarcoma, and all were in CR at transplant. Median time to relapse
was 6 months (2–59). Sixteen of 18 (89%) relapsed within 1 year. Median overall survival was 10 months (0–137). A trend towards improved survival (P=0.07) was evident for patients in CR prior to autotransplant.Conclusions: Stem cell autotransplantation does not benefit most patients with sarcoma. A subgroup of high‐risk patients in CR may fare better and warrant further study.
The Role of Postoperative Irradiation in the Treatment of Locally Recurrent Incompletely Resected Extra‐Abdominal Desmoid Tumors
Authors: Fontanesi James , Mott Michael P. , Kraut Michael J. , Lucas David P. , Miller Peter R. 
Published: March 2011

Background: To define the efficacy of postoperative irradiation in patients with recurrent extra‐abdominal desmoid tumors in whom surgical intervention has resulted in microscopically or grossly positive surgical margins.Methods: A retrospective analysis was performed on all patients referred to the department of radiation oncology at the Detroit Medical Center with a diagnosis of recurrent extra‐abdominal desmoid tumor. This analysis includes all patients seen from 1 January 1990 through 31 December 1999. A total of 11 patients were treated to 13 sites. Ten had microscopically positive margins and three had gross residual disease. Three patients were noted to have multifocal disease at the time of initial representation. Local control, survival, follow‐up, and subsequent development of new tumors are
measured from the last day of treatment with irradiation.Results: Thirteen sites were treated. Seven patients had received chemotherapy/hormonal therapy prior to surgery and/or
irradiation. The most commonly used drug was tamoxifen (n=6). The type of radiation delivered included external beam irradiation alone (n=3), combined external beam irradiation and brachytherapy (n=4), brachytherapy alone (n=3) and 252‐Cf neutron brachytherapy alone (n=3). Follow‐up has ranged from 29 to 115 months (median=76 months). Three patients have failed locally at 17, 24 and 29 months. One of these was treated for gross residual disease. No patient has died of tumor‐related causes. Salvage at the failed sites was possible in twom of three with re‐irradiation using external neutrons and/or aggressive surgical intervention and systemic therapy. Complications were most often noted to include decrease range in motion, especially in joint areas, and skin reactions which were normal in presentation. In one site there was development soft tissue necrosis.Conclusion: Based on our experience we recommend postoperative irradiation for all recurrent extra‐abdominal desmoid lesions with microscopically or grossly positive surgical margins. Furthermore, patients with recurrent desmoid tumors involving the bony structures of the hand or feet are poor candidates for brachytherapy alone. For patients with extremity lesions, brachytherapy may be a reasonable treatment option provided adequate margins around the tumor bed are covered. The continued recommended use of irradiation in this group of patients is warranted.