bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amino acid substitutions in hepatitis C virus core region predict hepatocarcinogenesis following eradication of HCV RNA by antiviral therapy
Authors: Akuta Norio , Suzuki Fumitaka , Hirakawa Miharu , Kawamura Yusuke , Sezaki Hitomi , Suzuki Yoshiyuki , Hosaka Tetsuya , Kobayashi Masahiro , Kobayashi Mariko , Saitoh Satoshi , Arase Yasuji , Ikeda Kenji , Kumada Hiromitsu 
Published: April 2011

AbstractSubstitution of amino acid (aa) 70 and/or 91 in the core region of HCV genotype 1b (HCV‐1b) is an important predictor of hepatocarcinogenesis, but its impact on the development of hepatocellular carcinoma (HCC) following eradication of HCV RNA by antiviral therapy is not clear. 1,273 patients with HCV‐related chronic liver disease, with sustained virological response, defined as negative HCV RNA at 24 weeks after cessation of interferon monotherapy or interferon plus ribavirin combination therapy, were included in a follow‐up study to evaluate the impact of aa substitution in the core region on hepatocarcinogenesis. Twenty six patients developed HCC during the follow‐up. The cumulative rates of new HCC were 3.2%, 4.8%, and 8.6% at the end of 5, 10, and 15 years, respectively. The rates in patients infected with HCV‐1b/Gln70(His70) [glutamine (histidine) at aa 70] were significantly higher than in patients infected with HCV‐1b/Arg70 (arginine at aa 70) (P = 0.007; log‐rank test) and HCV‐2a/2b (P < 0.001; log‐rank test). The rates in patients infected with HCV‐1b/Arg70 were not significantly higher than in those infected with HCV‐2a/2b (P = 0.617; log‐rank test). Multivariate analysis identified HCV‐1b/Gln70(His70) (HR 10.5, P < 0.001), advanced fibrosis (HR 9.03, P = 0.002), and old age (HR 3.09, P = 0.066) as determinants of hepatocarcinogenesis. In conclusion, aa substitution in the core region of HCV‐1b at the start of antiviral therapy is an important predictor of HCC following eradication of HCV RNA. This study emphasizes the importance of detection of aa substitutions in the core region before antiviral therapy. J. Med. Virol. 83:1016–1022, 2011. © 2011 Wiley‐Liss, Inc.
Intra‐individual processes linking work and employee well‐being: Introduction into the special issue
Authors: Sonnentag Sabine , Ilies Remus 
Published: April 2011

AbstractThis special issue presents eight empirical papers on intra‐individual processes related to employee well‐being in organization with the most of the papers featuring a within‐person approach. The studies examine trajectories of affect, affective predictors of organizational citizenship behavior and creativity, intra‐individual changes in flow at work, and also address longer‐term changes and the role of implicit attitudes. Overall, the studies demonstrate that affective states and well‐being substantially fluctuates within persons, and that these fluctuations are meaningfully related to outcomes that matter for organizations. Copyright © 2011 John Wiley & Sons, Ltd.
Deciphering signals of late Holocene fluvial and aeolian supply from a shelf sediment depocentre off Senegal (north‐west Africa)
Authors: Nizou Jean , Hanebuth Till J.J. , Vogt Christoph 
Published: April 2011

AbstractHigh‐resolution climatic records of the late Holocene along the north‐west African continental margin are scarce. Here we combine sediment grain size, elemental distribution and mineral assemblage data to trace dust and riverine sources at a shallow‐marine sediment depocentre in the vicinity of the Senegal River mouth. The aim is to understand how these terrigenous components reflect climate variability during the late Holocene. Major element contents were measured and mineral identification was performed on three sub‐fractions of our sediment core: (i) fluvial material < 2 µm, (ii) aeolian material of 18–63 µm and (iii) a sub‐fraction of dual‐origin material of 2–18 µm. Results show that more than 80% of the total Al and Fe terrigenous bulk content is present in the fluviogenic fraction. In contrast, Ti, K and Si cannot be considered as proxies for one specific source off Senegal. The Al/Ca ratio, recording the continental river runoff, reveals two dry periods from 3010 to 2750 cal a BP and from 1900 to 1000 cal a BP, and two main humid periods from 2750 to 1900 cal a BP and from 1000 to 700 cal a BP. The match between (i) intervals of low river runoff inferred by low Al/Ca values, (ii) reduced river discharge inferred by integrated palynological data from offshore Senegal and (iii) periods of enhanced dune reactivation in Mali confirms this interpretation. Copyright © 2011 John Wiley & Sons, Ltd.
Orthogonal test design for optimization of suitable conditions to separate C‐phycocyanin from Spirulina platensis by high‐speed counter‐current chromatography using reverse micelle solvent system
Authors: Yin Lianhong , Xu Lina , Yu Kun , Zhen Yuhong , Han Xu , Xu Youwei , Qi Yan , Peng Jinyong , Tan Aiping 
Published: April 2011

AbstractHigh‐speed counter‐current chromatography (HSCCC) was applied to separate C‐phycocyanin (C‐PC) from Spirulina platensis in the article. The suitable conditions were optimized by an orthogonal test design (L9(3)3), including the stationary phase of reverse micelle solvent system (0.10 g/mL cetyltrimethylammonium bromide [CTAB]/isooctane–hexylalcohol), mobile phase A (0.05 mol/L sodium phosphate buffer, pH 4.0, containing 0.2 mol/L KCl) and mobile phase B (0.05 mol/L sodium phosphate buffer, pH 8.0, containing 0.4 mol/L KCl). Under the selected conditions, 78.7 mg protein was purified from 200 mg crude extract of S. platensis, and the purity of the product was 4.25 based on the absorbance ratio of A620/A280, which was increased 6.85 times compared with the crude extract. Then, the protein was identified to be C‐PC by MALDI‐TOF/TOF‐MS and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) analysis compared with the standard. The application of HSCCC used in the separation of C‐PC from S. platensis was first reported in the article. Furthermore, three kinds of tumor cell lines including human hepatoma cell line SMMC‐7721, human ovarian carcinoma cell line ES‐2, and human lung adenocarcinoma cell line SPCA‐1 were used to evaluate the anticancer activities of the separated product, and the results showed that the separated C‐PC had excellent anti‐tumor actions with the IC50 values at 2.998, 4.854, and 8.423 μg/mL, respectively, for 48 h treatment. The outcome indicates that an effective method for C‐PC purification by HSCCC has been established.
Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods
Authors: Stankevičius Mantas , Akuņeca Ieva , Jãkobsone Ida , Maruška Audrius 
Published: April 2011

AbstractComparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion (Allium cepa), parsley (Petroselinum crispum) roots and leaves, celery (Apium graveolens) roots and leaves and leaves of dill (Anethum graveolens) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) RSA of extracts with the results obtained using reversed‐phase chromatographic separation with on‐line post‐column radical scavenging reaction detection, good correlation was obtained (R2=0.848). Studies using HPLC system with electrochemical detector showed that bioactive phytochemicals can be separated and antioxidant activities of individual compounds evaluated without the need of a complex HPLC system with reaction detector. The results obtained using electrochemical detection correlate with the RSA assayed using spectrophotometric method (R2=0.893).
Factor V Leiden associated with flap loss in microsurgical breast reconstruction
Authors: Khansa Ibrahim , Colakoglu Salih , Tomich David C. , Nguyen Minh‐Doan , Lee Bernard T. 
Published: April 2011

AbstractTwo cases are reported of flap loss following microsurgical perforator flap breast reconstruction in patients diagnosed with a factor V Leiden mutation. Factor V Leiden is the most common inherited cause of hypercoagulability, leading to an increased risk of thrombotic events. The first patient underwent a deep inferior epigastric artery perforator flap and then had recurrent arterial thrombosis both intraoperatively and postoperatively. This patient was subsequently diagnosed with a factor V Leiden mutation. The second patient had a known factor V Leiden mutation and underwent a superior gluteal artery perforator flap, which developed thrombosis and flap loss 2 days later. Preoperative assessment of a personal or family history of unexplained venous or arterial thrombosis should prompt suspicion of a factor V Leiden mutation. This mutation places patients at high risk for thromboembolic events in microvascular breast reconstruction, particularly when oral contraceptives or tamoxifen are used in conjunction. © 2011 Wiley‐Liss, Inc. Microsurgery, 2011.
Repair of nerve defect with acellular nerve graft supplemented by bone marrow stromal cells in mice
Authors: Zhao Zhe , Wang Yu , Peng Jiang , Ren Zhiwu , Zhan Shengfeng , Liu Yan , Zhao Bin , Zhao Qing , Zhang Li , Guo Quanyi , Xu Wenjing , Lu Shibi 
Published: April 2011

AbstractThe acellular nerve graft that can provide internal structure and extracellular matrix components of the nerve is an alternative for repair of peripheral nerve defects. However, results of the acellular nerve grafting for nerve repair still remain inconsistent. This study aimed to investigate if supplementing bone marrow mesenchymal stromal cells (MSCs) could improve the results of nerve repair with the acellular nerve graft in a 10‐mm sciatic nerve defect model in mice. Eighteen mice were divided into three groups (n = 6 for each group) for nerve repairs with the nerve autograft, the acellular nerve graft, and the acellular nerve graft by supplemented with MSCs (5 × 105) fibrin glue around the graft. The mouse static sciatic index was evaluated by walking‐track testing every 2 weeks. The weight preservation of the triceps surae muscles and histomorphometric assessment of triceps surae muscles and repaired nerves were examined at week 8. The results showed that the nerve repair by the nerve autografting obtained the best functional recovery of limb. The nerve repair with the acellular nerve graft supplemented with MSCs achieved better functional recovery and higher axon number than that with the acellular nerve graft alone at week 8 postoperatively. The results indicated that supplementing MSCs might help to improve nerve regeneration and functional recovery in repair of the nerve defect with the acellular nerve graft. © 2011 Wiley‐Liss, Inc. Microsurgery, 2011.
Tongue reconstruction with the gracilis myocutaneous free flap
Authors: Calabrese Luca , Saito Akira , Navach Valeria , Bruschini Roberto , Saito Noriko , Zurlo Valeria , Ostuni Angelo , Garusi Cristina 
Published: April 2011

AbstractWe describe our experience in tongue reconstruction using the transverse gracilis myocutaneous (TMG) free flap after major demolitive surgery for advanced cancer. This technique was used in 10 patients: seven underwent total glossectomy and three partial glossectomy. In eight patients we performed motor reinnervation attempting to maintain muscular trophism and gain long‐term volumetric stability. The follow‐up period ranged from 6 to 28 months. The overall flap survival was 100%. Nine out of 10 patients resumed oral intake. Our preliminary experience shows that this flap is a good reconstructive option for total glossectomy patients, whereas it is less suited for reconstruction of hemiglossectomy defects. Functional and objective evaluation of the tongue reconstructed with TMG free flap requires further and standardized evaluation. © 2011 Wiley‐Liss, Inc. Microsurgery, 2011.
The importance of the superficial venous anatomy of the abdominal wall in planning a superficial inferior epigastric artery (SIEA) flap: Case report and clinical study
Authors: Rozen Warren M , Chubb Daniel , Whitaker Iain S , Ashton Mark W 
Published: April 2011

AbstractThe importance of the venous drainage of the anterior abdominal wall to free tissue transfer in deep inferior epigastric artery perforator flap surgery has been highlighted in several recent publications in this journal, however the same attention has not been given to superficial inferior epigastric artery (SIEA) flaps, in which the flap necessarily relies on the superficial venous drainage. We describe a unique case, in which the presence of two superficial inferior epigastric veins (SIEVs) draining into separate venous trunks was identified. The use of only one trunk led to a well‐demarcated zone of venous congestion. A clinical study was also conducted, assessing 200 hemiabdominal walls with preoperative computed tomographic angiography imaging. The presence of more than a single major SIEV trunk was present in 80 hemiabdominal walls (40% of overall sides). There was considerable variability in the source of drainage of the SIEV, draining variably into the deep inferior epigastric vein, the great saphenous vein, the saphenous bulb, a common trunk with the superficial circumflex iliac vein or a common trunk with a second branch of the SIEV. These findings highlight the considerable variation in the number of SIEV trunks as well as their source of regional drainage, and show the importance of consideration of such variation. © 2011 Wiley‐Liss, Inc. Microsurgery, 2011.
Lateral intercostal artery perforator flap used in salvage of exposed tissue expander of breast: Case report
Authors: Unal Cigdem , Gercek Huseyin , Yasar Emrah Kagan , Utkan Zafer 
Published: April 2011

AbstractManagement of an exposed tissue expander in breast reconstruction patients remains a challenging problem. For large defects that cannot be repaired primarily, local flap options are limited. In this case report, we describe the use of lateral intercostal artery perforator (LICAP) flap in salvage of an exposed tissue expander of a patient who had delayed immediate breast reconstruction after mastectomy. The postoperative recovery was uneventful and tissue expansion followed by radiotherapy was well tolerated by the flap. We believe this is the first article to describe the use of LICAP flap in salvage of an exposed tissue expander of the breast due to mastectomy flap necrosis in the early postoperative period. © 2011 Wiley‐Liss, Inc. Microsurgery, 2011.
A practical two‐term acceleration algorithm for linear systems
Authors: Wang Chuan‐Long , Meng Guo‐Yan 
Published: April 2011

SUMMARYIn this paper, a practical two‐term acceleration algorithm is proposed, the interval of the parameter which guarantees the convergence of the acceleration algorithm is analyzed in detail. Further, the acceleration ratio of the new acceleration algorithm is obtained in advance. The new acceleration algorithm is less sensitive to the parameter than the Chebyshev semi‐iterative method. Finally, some numerical examples show that the accelerated algorithm is effective. Copyright © 2011 John Wiley & Sons, Ltd.
A nonintrusive stochastic multiscale solver
Authors: Fish Jacob , Wu Wei 
Published: April 2011

AbstractIn this paper, we describe a practical nonintrusive multiscale solver that permits consideration of uncertainties in heterogeneous materials without exhausting the available computational resources. The computational complexity of analyzing heterogeneous material systems is governed by the physical and probability spaces at multiple scales. To deal with these large spaces, we employ reduced order homogenization approach in combination with the Karhunen–Loeve expansion and stochastic collocation method based on sparse grid. The resulting nonintrusive multiscale solver, which is aimed at providing practical solutions for complex multiscale stochastic problems, has been verified against the Latin Hypercube Monte–Carlo method. Copyright © 2011 John Wiley & Sons, Ltd.
Reduced Paneth cell antimicrobial protein levels correlate with activation of the unfolded protein response in the gut of obese individuals
Authors: Hodin Caroline M , Verdam Froukje J , Grootjans Joep , Rensen Sander S , Verheyen Fons K , Dejong Cornelis HC , Buurman Wim A , Greve Jan Willem , Lenaerts Kaatje 
Published: April 2011

AbstractThe intestinal microbiota is increasingly acknowledged to play a crucial role in the development of obesity. A shift in intestinal microbiota composition favouring the presence of Firmicutes over Bacteroidetes has been observed in obese subjects. A similar shift has been reported in mice with deficiency of active Paneth cell α‐defensins. We aimed at investigating changes in Paneth cell antimicrobial levels in the gut of obese subjects. Next, we studied activation of the unfolded protein response (UPR) as a possible mechanism involved in altered Paneth cell function. Paneth cell numbers were counted in jejunal sections of 15 severely obese (BMI > 35) and 15 normal weight subjects. Expression of Paneth cell antimicrobials human α‐defensin 5 (HD5) and lysozyme were investigated using immunohistochemistry, qPCR, and western blot. Activation of the UPR was assessed with western blot. Severely obese subjects showed decreased protein levels of both HD5 and lysozyme, while Paneth cell numbers were unchanged. Lysozyme protein levels correlated inversely with BMI. Increased expression of HD5 (DEFA5) and lysozyme (LYZ) transcripts in the intestine of obese subjects prompted us to investigate a possible translational block caused by UPR activation. Binding protein (BiP) and activating transcription factor 4 (ATF4) levels were increased, confirming activation of the UPR in the gut of obese subjects. Furthermore, levels of both proteins correlated with BMI. Involvement of the UPR in the lowered antimicrobial protein levels in obese subjects was strongly suggested by a negative correlation between BiP levels and lysozyme levels. Additionally, indications of ER stress were apparent in Paneth cells of obese subjects. Our findings provide the first evidence for altered Paneth cell function in obesity, which may have important implications for the obesity‐associated shift in microbiota composition. In addition, we show activation of the UPR in the intestine of obese subjects, which may underlie the observed Paneth cell compromise. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Cystogenic potential of CD133+ progenitor cells of human polycystic kidneys
Authors: Carvalhosa Raquel , Deambrosis Ilaria , Carrera Paola , Pasquino Chiara , Rigo Francesca , Ferrari Maurizio , Lasaponara Fedele , Ranghino Andrea , Biancone Luigi , Segoloni Giuseppe , Bussolati Benedetta , Camussi Giovanni 
Published: April 2011

AbstractIn autosomal dominant polycystic kidney disease, cysts arise focally and disrupt normal renal tissue leading to renal failure. In the present study, we show that cyst‐lining cells express the stem cell marker CD133. CD133+ progenitor cells isolated from polycystic kidney, carrying mutations of PKD genes, showed a dedifferentiated phenotype similar to CD133+ progenitor cells from normal kidney. However, these cells were more proliferative and presented a defective epithelial differentiation phenotype with respect to normal renal CD133+ cells as they were not able to express all tubular epithelial cell markers when cultured in epithelial differentiation medium. Polycystic CD133+ cells, in contrast to normal renal CD133+ cells, formed cysts in vitro in a three‐dimensional culture system and in vivo when injected subcutaneously within Matrigel in SCID mice. Rapamycin treatment reduced in vitro proliferation of polycystic CD133+ cells and decreased cystogenesis both in vitro and in vivo. The in vitro epithelial differentiation was only partially improved by rapamycin. These results indicate that polycystic CD133+ cells retain a dedifferentiated phenotype and the ability to generate cysts. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Tid1, CHIP and ErbB2 interactions and their prognostic implications for breast cancer patients
Authors: Jan Chia‐Ing , Yu Cheng‐Chia , Hung Mien‐Chie , Harn Horng‐Jyr , Nieh Shin , Lee Herng‐Sheng , Lou Mary Ann , Wu Yi‐Chen , Chen Chi‐Yuan , Huang Chi‐Yang , Chen Fei‐Na , Lo Jeng‐Fan 
Published: April 2011

AbstractErbB2 (HER2/neu) is overexpressed in about 25–30% of breast malignancies, and up‐regulation of ErbB2 in breast cancer patients is associated with poor prognosis. It is known that the carboxyl terminus of heat shock cognate 70 interacting protein (CHIP) efficiently down‐regulates ErbB2 in vitro. Human tumourous imaginal disc 1 (Tid1, DnaJa3), a co‐chaperone of heat shock protein 70 (Hsp70), also suppresses ErbB2 expression in breast cancer cell lines. However, the intracellular interactions among Tid1, CHIP, and ErbB2 remain elusive, and the utilization of Tid1 and CHIP as breast cancer biomarkers has never been proposed. Herein, we analysed the expression and correlations among Tid1, CHIP, and ErbB2 in a total of 183 breast cancer histology sections, including 30 fresh tissue specimens, using immunohistochemistry (IHC) and immunoblotting assay. A computerized image analysis system was used for IHC scoring and determining relative immunoblot intensity. The immunohistochemical expression of Tid1 and CHIP were positively correlated with each other but were both inversely correlated to that of ErbB2. Odds ratio analyses showed that lower expression of Tid1 has a relatively higher risk of unfavourable tumour grade, later pathological stage, larger tumour size, and microscopic features of a more malignant histology including lymphovascular invasion, stromal inflammatory response, and tumour necrosis. Expression of CHIP displayed similar characteristics. Furthermore, expression of Tid1 and/or CHIP increases patients' 10‐year overall and disease‐free survival rate. Empirically, we also demonstrated that Tid1, CHIP, and ErbB2 interacted with each other through immunofluorescence or co‐immunoprecipitation analyses. Functionally, Tid1 and CHIP acted synergistically to degrade ErbB2 in vitro. Conversely, Tid1 cannot compensate for the loss of proteolytic function noted in CHIP mutations for degradation of ErbB2. Overall, our data suggest that Tid1 and CHIP play pivotal roles in affecting the levels of ErbB2 protein, and that both are significant prognostic indicators of breast cancer patient survival. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.