bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of non‐thermal processing on the nutritional quality and bioactive properties of industrial hempseed flours and protein isolate
Authors: Nguyen Anh T. L. , Aryee Alberta N. A. 
Published: April 2024

AbstractThere is an unmet demand for plant‐based ingredients with desirable nutritional, techno‐functional properties, and health benefits. In this study, the composition, nutritional quality, and bioactives in industrial hempseed flours and protein isolate generated by milling, germination, isoelectric precipitation (IEP), and enzyme‐assisted extraction (EAE) were evaluated. Moisture, ash, fat, protein, phytic acid, tannin, and trypsin inhibitor content of the hempseed flours and protein isolate were 2.80%–6.46%, 5.07%–28.89%, 0.00%–31.44%, 22.71%–89.94%, 0.55%–1.05%, 274.24–1300.76 μg/g, and 0.00–42.66 U/g, respectively. IEP resulted in the highest protein content (89.94%), indicative of its effective to isolate hempseed protein. Germination and IEP significantly reduced phytic acid and tannin contents by 1.53‐ and 3.63‐fold, respectively. All processing methods improved in vitro protein digestibility (IVPD). SDS‐PAGE analysis revealed comparable band patterns in milled and protein isolate, with a strong 50 kDa band attributed as edestin. Amino acid analysis showed that EAE augmented total essential amino acids, particularly protease. Milling and pronase treatment yielded the highest and lowest IVPD‐corrected amino acid score (IVPDCAAS) of 76% and 47%, respectively. Milled and germinated flours contained varying amounts of γ‐tocopherol, lutein, zeaxanthin, α‐carotene, and β‐carotene. Germinated flour exhibited elevated levels of total phenolic (14.36 mg/g), and flavonoid (1.76 mg/g) contents, FRAP, TEAC, and DPPH compared to the other flours suggesting superior antioxidant capacity. Strong positive correlations (r >0.70) were found between IVPD and phytic acid for the protein isolate, total phenolic content, and FRAP for IEP and germinated flours. Overall, hempseed flours with diverse properties could be produced using non‐thermal processing.
A method for pH food dependent shelf‐life prediction of intelligent sustainable packaging device using artificial neural networks
Authors: Brazolin Isadora Fernandez , Sousa Felipe Matheus Mota , dos Santos Jackson Wesley Silva , Concha Viktor Oswaldo Cárdenas , da Silva Flavio Vasconcelos , Yoshida Cristiana Maria Pedroso 
Published: April 2024

AbstractIntelligent packaging systems can contribute to easily informing the quality of products. Tools such as artificial neural networks (ANN) have the potential to be used in the development of intelligent packaging. The objective of this work was the development and training of an ANN to facilitate the control of foods from the pH variation based on a sustainable colorimetric indicator of chitosan‐anthocyanin. The pH intelligent films were prepared with different concentrations of chitosan (Cch, 0.5%, 1.0%, and 2.0%, w/w) and anthocyanin (Cath, 0.5%, 1.0%, and 2.0%, w/w). The films were characterized by water solubility, mechanical properties, and thermal analyses. The colorimetric efficiency of the pH intelligent films was measured by immersing the material in a wide pH range (1.20–12.58) buffer solution, determining the color parameters L*, a*, and b*. From the experimental results, a database was built to develop an empirical multivariable model based on ANN. Higher Cch increases the solubility and resistance of intelligent films. Color variation was better identified in films containing Cath = 0.5%. The ANN presented assertiveness of 79%, showing that classification algorithms based on colorimetric measurements can be exploited to indicate alterations in food products resulting from pH variation.
Identification of active main metabolites of anti‐infective inhibitors of the macrophage infectivity potentiator protein by liquid chromatography using mass detection
Authors: Lohr Theresa , Scheuplein Nicolas Julian , Jenkins Christopher , Norville Isobel , Erk Christine , Stapf Maximilian , Kirchner Lukas , Sarkar‐Tyson Mitali , Holzgrabe Ulrike 
Published: April 2024

AbstractDue to increasing antibiotic resistance, the development of anti‐infectives with new mechanisms of action is crucial. Virulence factors such as the “macrophage infectivity potentiator” (Mip) protein, which catalyzes the folding of proline‐containing proteins by means of their cis–trans isomerase (PPIase) activity, have come into focus as a potential new target. Since the inhibition of Mip by small molecules has been shown to lead to reduced virulence and survival in vitro, especially of Gram‐negative bacteria such as Burkholderia pseudomallei (Bp), Neisseria meningitidis (Nm), and Neisseria gonorrhoeae (Ng), or Coxiella burnetii (Cb), among many others, a library of Mip inhibitors was developed. As drug metabolism has a significant impact on the overall therapeutic outcome, this report describes the biotransformation of the most potent Mip inhibitors. Therefore, the anti‐infectives were treated using human liver microsomes in vitro. Liquid chromatography with tandem mass spectrometry (LC/MS‐MS) methods were applied to identify the metabolites and quantify the metabolic degradation of the hit compounds. Active metabolites, N‐oxides, were found, leading to new opportunities for further drug development.
Synthesis of the chromone‐thiosemicarbazone scaffold as promising α‐glucosidase inhibitors: An in vitro and in silico approach toward antidiabetic drug design
Authors: Alharthy Rima D. , Khalid Sana , Fatima Shamool , Ullah Saeed , Khan Ajmal , Mali Suraj N. , Jawarkar Rahul D. , Dhabarde Sanjay S. , Kashtoh Hamdy , Taslimi Parham , Al‐Harrasi Ahmed , Shafiq Zahid , Boshta Nader M. 
Published: April 2024

AbstractDiabetes is a serious metabolic disorder affecting individuals of all age groups and prevails globally due to the failure of previous treatments. This study aims to address the most prevalent form of type 2 diabetes mellitus (T2DM) by reporting on the design, synthesis, and in vitro as well as in silico evaluation of chromone‐based thiosemicarbazones as potential α‐glucosidase inhibitors. In vitro experiments showed that the tested compounds were significantly more potent than the standard acarbose, with the lead compound 3n exhibiting an IC50 value of 0.40 ± 0.02 μM, ~2183‐fold higher than acarbose having an IC50 of 873.34 ± 1.67 μM. A kinetic mechanism analysis demonstrated that compound 3n exhibited reversible inhibition of α‐glucosidase. To gain deeper insights, in silico molecular docking, pharmacokinetics, and molecular dynamics simulations were conducted for the investigation of the interactions, orientation, stability, and conformation of the synthesized compounds within the active pocket of α‐glucosidase.
E2F transcription factors promote tumorigenicity in pancreatic ductal adenocarcinoma
Authors: Bertonnier‐Brouty Ludivine , Andersson Jonas , Kaprio Tuomas , Hagström Jaana , Bsharat Sara , Asplund Olof , Hatem Gad , Haglund Caj , Seppänen Hanna , Prasad Rashmi B. , Artner Isabella 
Published: April 2024

AbstractBackgroundPancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers with limited treatment options, illustrating an urgent need to identify new drugable targets in PDACs.ObjectiveUsing the similarities between tumor development and normal embryonic development, which is accompanied by rapid cell expansion, we aimed to identify and characterize embryonic signaling pathways that were reinitiated during tumor formation and expansion.Methods and ResultsHere, we report that the transcription factors E2F1 and E2F8 are potential key regulators in PDAC. E2F1 and E2F8 RNA expression is mainly localized in proliferating cells in the developing pancreas and in malignant ductal cells in PDAC. Silencing of E2F1 and E2F8 in PANC‐1 pancreatic tumor cells inhibited cell proliferation and impaired cell spreading and migration. Moreover, loss of E2F1 also affected cell viability and apoptosis with E2F expression in PDAC tissues correlating with expression of apoptosis and mitosis pathway genes, suggesting that E2F factors promote cell cycle regulation and tumorigenesis in PDAC cells.ConclusionOur findings illustrate that E2F1 and E2F8 transcription factors are expressed in pancreatic progenitor and PDAC cells, where they contribute to tumor cell expansion by regulation of cell proliferation, viability, and cell migration making these genes attractive therapeutic targets and potential prognostic markers for pancreatic cancer.
Identifying priority areas to support primary care engagement in breast cancer survivorship care: A Delphi study
Authors: Mikesell Lisa , O'Malley Denalee M. , Kurtzman Rachel T. , Howard Jenna , Bates Benjamin , Hemler Jennifer R. , Fadem Sarah J. , Ferrante Jeanne M. , Bator Alicja , Hudson Shawna V. , Crabtree Benjamin F. 
Published: April 2024

AbstractIntroductionExisting approaches in cancer survivorship care delivery have proven to be insufficient to engage primary care. This study aimed to identify stakeholder‐informed priorities to improve primary care engagement in breast cancer survivorship care.MethodsExperts in U.S. cancer survivorship care delivery were invited to participate in a 4‐round online Delphi panel to identify and evaluate priorities for defining and fostering primary care's engagement in breast cancer survivorship. Panelists were asked to identify and then assess (ratings of 1–9) the importance and feasibility of priority items to support primary care engagement in survivorship. Panelists were asked to review the group results and reevaluate the importance and feasibility of each item, aiming to reach consensus.ResultsRespondent panelists (n = 23, response rate 57.5%) identified 31 priority items to support survivorship care. Panelists consistently rated three items most important (scored 9) but with uncertain feasibility (scored 5–6). These items emphasized the need to foster connections and improve communication between primary care and oncology. Panelists reached consensus on four items evaluated as important and feasible: (1) educating patients on survivorship, (2) enabling screening reminders and monitoring alerts in the electronic medical record, (3) identifying patient resources for clinicians to recommend, and (4) distributing accessible reference guides of common breast cancer drugs.ConclusionRole clarity and communication between oncology and primary care were rated as most important; however, uncertainty about feasibility remains. These findings indicate that cross‐disciplinary capacity building to address feasibility issues may be needed to make the most important priority items actionable in primary care.
Wound Healing Activity of Topical Herbal Gels Containing Barringtonia acutangula Fruit Extract: In silico and In vivo Studies
Authors: Priyanka Kumari , Sahoo Rudra Narayan , Nanda Ashirbad , Kanhar Satish , Das Chandan , Sahu Abhijit , Naik Pradeep Kumar , Nayak Amit Kumar 
Published: April 2024

AbstractThe current study describes the efficacy of B. acutangula fruit extract in wound healing via incorporation within topical gels. B. acutangula fruit extract was produced by solvent extraction method. The bioactive extract was incorporated within Carbopol 940‐based topical gels, which were applied topically over the excision and incision wounds. The change in healing process was observed till 20 days. The percentages of closure of excision wound area were 92.89 % and 93.43 %, when treated with topical herbal gels containing B. acutangula fruit extract of 5 % and 10 %, respectively. The tensile strengths of incision area in rats treated with topical herbal gels containing 5 % and 10 % methanol extract of B. acutangula fruits were found to be 25±5.12 g and 30±4.10 g, respectively. The wound healing activity of topical herbal gels containing B. acutangula fruit extract in rats was found to be significant when compared with that of the reference standard and untreated groups. In addition, in silico studies suggested about good skin permeability and binding to the proteins responsible for delaying wound healing. It can be concluded that this topical herbal gels containing B. acutangula fruit extract could be used clinically for the treatment of wounds.
Stable CAAC‐Triazenes: A New Nitrogen Ligand System With Donor and Conformational Flexibility, and With Application in Olefin Activation Catalysis
Authors: Race James J. , Hudson Luke A. , Albrecht Martin 
Published: April 2024

AbstractN‐heterocyclic imines such as pyridylidene amines impart high catalytic activity when coordinated to a transition metal, largely imposed by their electronic flexibility. Here, this donor flexibility has been applied for the first time to CAAC‐based systems through the synthesis of CAAC‐triazenes. These new ligands offer a larger π‐conjugation that extends from the N‐heterocyclic carbene through three nitrogens rather than just one, as observed in N‐heterocyclic imines. We demonstrate the straightforward synthesis of three new CAAC‐triazenes containing different substituents on the terminal triazene nitrogen. These compounds are remarkably stable up to 120 °C without loss of N2 as typically observed with similar triazenes. E‐to‐Z isomerization within the triazene is instigated by UV light and is partially reversible dependent on the triazene substituent. The quinoline‐substituted CAAC‐triazene 1‐Q has been applied as an L,L’‐type ligand in the synthesis of [PdCl2(1‐Q)], [PdCl(Me)(1‐Q)] and [Pd(Me)(H2O(1‐Q)]+. E‐to‐Z ligand isomerization also occurs when coordinated to PdCl2, providing access to on‐metal manipulation. The cationic complex [PdMe(H2O)(1‐Q)]+ is a precatalyst for oligomerization of ethylene to form initially 2‐butene and subsequently linear and branched C8−C12 products from butene activation. Moreover, isomerization of 1‐hexene takes place efficiently with exceptionally low catalyst loading (10 ppm) and up to 74,000 turnover numbers.
Applicability and Validity of CFD‐based Compartment Models for One to Multiple Operating Points
Authors: Schwarz Sebastian , Grünewald Marcus 
Published: April 2024

AbstractCFD‐based compartment (CPT) models offer a promising modeling method and trade‐off between computational effort and required accuracy. The models combine the relevant fluid dynamics from the computational expensive CFD and the incorporation of complex reaction mechanisms usually used in basic reactor models. However, one criticism is their reliance on specific operating points, rendering them invalid when boundary conditions change. This study explores using a CFD‐based CPT model for static mixing elements across multiple operating points by making use of the creeping flow. The mean age theory facilitates a comprehensive comparison between CFD and CPT models. Results indicate that within this flow region, the CPT model can be extrapolated to other operating points via linear scaling.
Electroosmotic Sensing of Uncharged Peptides and Differentiating Their Phosphorylated States Using Nanopores
Authors: Si Wei , Chen Jiayi , Zhang Zhen , Wu Gensheng , Zhao Jiajia , Sha Jingjie 
Published: April 2024

AbstractThe correct characterization and identification of different kinds of proteins is crucial for the survival and development of living organisms, and proteomics research promotes the analysis and understanding of future genome functions. Nanopore technique has been proved to accurately identify individual nucleotides. However, accurate and rapid protein sequencing is difficult due to the variability of protein structures that contains more than 20 amino acids, and it remains very challenging especially for uncharged peptides as they can not be electrophoretically driven through the nanopore. Graphene nanopores have the advantages of high accuracy, sensitivity and low cost in identifying protein phosphorylation modifications. Here, by using all‐atom molecular dynamics simulations, charged graphene nanopores are employed to electroosmotically capture and sense uncharged peptides. By further mimicking AFM manipulation of single molecules, it is also found that the uncharged peptides and their phosphorylated states could also be differentiated by both the ionic current and pulling force signals during their pulling processes through the nanopore with a slow and constant velocity. The results shows ability of using nanopores to detect and discriminate single amino acid and its phosphorylation, which is essential for the future low‐cost and high‐throughput sequencing of protein residues and their post‐translational modifications.
Corporate social responsibility practices and value creation through open innovation approach: Evidence from the STOXX Europe 600 Index
Authors: Martielli Francesco , Salvi Antonio , Doronzo Emanuele 
Published: April 2024

AbstractIn the current business setting, companies must adopt new practices to remain competitive due to complex products, changing market demands, and stakeholder pressures. Many successful businesses are turning to sustainability‐oriented innovations as a means of both increasing their growth potential and engaging in corporate social responsibility practices. This approach benefits both the company and society. However, the full potential of implementing sustainability practices through open innovation has yet to be fully explored, and the impact on firm value is unclear. Additionally, there is currently a lack of international standards for representing sustainability as an open innovation approach. This study aims to shed light on the potential benefits of adopting sustainable practices through the open innovation approach. We highlight the value that sustainable innovation can create and analyze a sample of European‐listed companies from the STOXX Europe 600 Index from 2011 to 2020. Our findings show that the ESG score best represents sustainability as an open innovation approach. Moreover, we demonstrate that adopting sustainability as an open innovation approach practice positively impacts firm value. This suggests that companies can enhance their value and promote sustainable innovation success by embracing this approach. Overall, this paper contributes to the literature on sustainability and open innovation, specifically within the legitimacy theory framework. It emphasizes that stakeholder pressure to build a more sustainable and ethical economic system presents a challenge that can also be an opportunity for companies.
Identification and characterisation of novel CAR‐T cells to target IL13Rα2 positive human glioma in vitro and in vivo
Authors: Leland Pamela , Degheidy Heba , Lea Ashley , Bauer Steven R. , Puri Raj K. , Joshi Bharat H. 
Published: April 2024

AbstractBackgroundPreviously, we discovered that human solid tumours, but not normal human tissues, preferentially overexpress interleukin‐13Receptor alpha2, a high binding receptor for IL‐13. To develop novel anti‐cancer approaches, we constructed a chimeric antigen receptor construct using a high binding and codon optimised scFv‐IL‐13Rα2 fragment fused with CD3ζ and co‐stimulatory cytoplasmic domains of CD28 and 4‐1BB.MethodsWe developed a scFv clone, designated 14‐1, by biopanning the bound scFv phages using huIL‐13Rα2Fc chimeric protein and compared its binding with our previously published clone 4‐1. We performed bioinformatic analyses for complementary determining regions (CDR) framework and residue analyses of the light and heavy chains. This construct was packaged with helper plasmids to produce CAR‐lentivirus and transduced human Jurkat T or activated T cells from peripheral blood mononuclear cells (PBMCs) to produce CAR‐T cells and tested for their quality attributes in vitro and in vivo. Serum enzymes including body weight from non‐tumour bearing mice were tested for assessing general toxicity of CAR‐T cells.ResultsThe binding of 14‐1 clone is to IL‐13Rα2Fc‐chimeric protein is ∼5 times higher than our previous clone 4‐1. The 14‐1‐CAR‐T cells grew exponentially in the presence of cytokines and maintained phenotype and biological attributes such as cell viability, potency, migration and T cell activation. Clone 14‐1 migrated to IL‐13Rα2Fc and cell free supernatants only from IL‐13Rα2+ve confluent glioma tumour cells in a chemotaxis assay. scFv‐IL‐13Rα2‐CAR‐T cells specifically killed IL‐13Rα2+ve but not IL‐13Rα2‐ve tumour cells in vitro and selectively caused significant release of IFN‐γ only from IL‐13Rα2+ve co‐cultures. These CAR‐T cells regressed IL‐13Rα2+ve glioma xenografts in vivo without any general toxicity. In contrast, the IL‐13Rα2 gene knocked‐down U251 and U87 xenografts failed to respond to the CAR‐T therapy.ConclusionTaken together, we conclude that the novel scFv‐IL‐13Rα2 CAR‐T cell therapy may offer an effective therapeutic option after designing a careful pre‐clinical and clinical study.
‘Acceptance of diversity’…but not too much? (Restricted) pathways towards transgender and intersex inclusivity in sex education curriculum guidelines
Authors: Fedorchenko Ayla 
Published: April 2024

AbstractPositive representation of LGBTQIA+ perspectives in curriculum can help improve well‐being of transgender and intersex students. However, research on sex education curriculum indicates that the experiences of intersex and transgender students are largely absent in the respective curriculum or constructed as other, pathologized or stigmatized. These discourses of silence and stigmatization negatively affect transgender and intersex students and prevent all students from getting informed and affirmative sex education on gender and body diversity. This study explores how curriculum documents for sex education in Europe and Germany may be facilitating or limiting the (de‐hierarchized) inclusion of diversity regarding gender and sex/body characteristics. The study employs a structuring qualitative content analysis. Thematic summaries and analysis through the lens of heteronormativity are used to examine how diverse subjectivities are made (in)visible, normalized or othered through the lexical choices and the general framing of the curriculum documents, as well as how binary categorizations are deconstructed and power relations subverted. Key findings indicate that some curricula, while strongly promoting diversity or explicitly including intersex and transgender as topics, are worded in ways that contribute to the normalization of cisgender and endosex experiences, while transgender or intersex perspectives are either fully silenced or othered. The data also reveal power‐critical approaches, in which transgender and intersex are affirmatively included, cis‐ and endonormativity is disrupted and which encourage a critical engagement with societal power relations.