bluuebunny/crossref_metadata_2025_split huggingface dataset:
research paper: Role of the cold shock domain protein A in the transcriptional regulation of HBG expression
Authors: Petruzzelli Raffaella , Gaudino Sara , Amendola Giovanni , Sessa Raffaele , Puzone Stella , Di Concilio Rosanna , D’Urzo Giovanna , Amendolara Maria , Izzo Paola , Grosso Michela 
Published: July 2010

SummaryImpaired switching from fetal haemoglobin (HbF) to adult globin gene expression leads to hereditary persistence of fetal haemoglobin (HPFH) in adult life. This is of prime interest because elevated HbF levels ameliorate β‐thalassaemia and sickle cell anaemia. Fetal haemoglobin levels are regulated by complex mechanisms involving factors linked or not to the β‐globin gene (HBB) locus. To search for factors putatively involved in the expression of the γ‐globin genes (HBG1, HBG2), we examined the reticulocyte transcriptome of three siblings who had different HbF levels and different degrees of β‐thalassaemia severity although they had the same ΗBA‐ and ΗΒB cluster genotypes. By mRNA differential display we isolated the cDNA coding for the cold shock domain protein A (CSDA), also known as dbpA, previously reported to interact in vitro with the HBG2 promoter. Expression studies performed in K562 and in primary erythroid cells showed an inverse relationship between HBG and CSDA expression levels. Functional studies performed by Chromatin Immunoprecipitation and reporter gene assays in K562 cells demonstrated that CSDA is able to bind the HBG2 promoter and suppress its expression. Therefore, our study demonstrated that CSDA is a trans‐acting repressor factor of HBG expression and modulates the HPFH phenotype.
review: Current status of stem cell mobilization
Authors: Gertz Morie A. 
Published: July 2010

SummaryNew advances in effective mobilization of peripheral blood stem cells have permitted a greater proportion of patients to benefit from autologous stem cell transplantation. In this review, the relative merits of peripheral blood and mobilized bone marrow are discussed. All available agents are reviewed. A critical assessment of the appropriate dosing and frequency of available growth factors is undertaken, and the most commonly used chemotherapy plus growth factor combinations are covered. Specific recommendations for patients who are poor mobilizers are dealt with including the role of plerixafor.
Plasma urocortin in acute myocardial infarction patients
Authors: Phrommintikul Arintaya , Sivasinprasasn Sivaporn , Lailerd Narissara , Chattipakorn Siriporn , Kuanprasert Srun , Chattipakorn Nipon 
Published: July 2010

Eur J Clin Invest 2010; 40 (10): 874–882AbstractBackground  Despite its proposed cardioprotective effect, the role of plasma urocortin in acute myocardial infarction (AMI) remains unknown. We investigated plasma profile of urocortin in AMI patients and evaluated its long‐term prognostic performance.Material and methods  Sixty‐six AMI patients and 21 healthy subjects were included in this study. Blood samples for urocortin were collected on days 0 (onset), 1, 3 and 5 and at 3 and 6 months. Primary endpoint was mortality within 1 year of follow‐up. Secondary endpoint was combined death and nonfatal adverse cardiac events (i.e. myocardial reinfarction, urgent revascularization or hospitalization due to heart failure) within 1 year.Results  During follow‐up at 1 year, 38 (57·6%) patients were alive without cardiac events, nine (13·6%) had nonfatal cardiac events and 17 (25·8%) died. Plasma urocortin in AMI patients were increased on days 0, 1, 3 and 5 (P < 0·05 vs. control). The receiver‐operating characteristic curve showed an area under curve (AUC) of day 0 urocortin to be 0·750 with 95% confidence interval (CI) of 0·619–0·881 (P = 0·004), whereas AUC of NT‐proBNP was 0·857 (95% CI, 0·722–0·992; P = 0·003). Sensitivity values for predicting the mortality of urocortin NT‐proBNP and a combined urocortin and NT‐proBNP were 0·81 (95% CI, 0·54–0·95), 0·86 (95% CI, 0·42–0·99) and 1·0 (95% CI, 0·56–1·0), respectively.Conclusions  Plasma urocortin level is elevated in AMI patients for 5 days from onset. High plasma urocortin within 24 h after the onset is associated with increased mortality. Combined urocortin and NT‐proBNP enhance prognostic performance in AMI patients.
Antibody injection in the egg yolk: maternal antibodies affect humoral immune response of the offspring
Authors: Staszewski Vincent , Siitari Heli 
Published: July 2010

Summary1. In vertebrate species, maternal antibodies specific for the pathogens to which mothers have been exposed can be transmitted to offspring before birth. This is assumed to be adaptive as these antibodies can be essential in protecting offspring early in life before their own immune system is fully developed. However, fitness consequences in natural conditions and the long‐term effects of these mechanisms have yet to be fully examined.2. Exploring the ecological and evolutionary implications of such maternal effects requires experimental manipulations to avoid potential confounding maternal effects. In birds, maternal antibodies are transmitted via the egg yolk – a self contained embryonic environment in which antibodies can be manipulated independently outside the maternal environment.3. Here we use a novel technique to extract and experimentally manipulate maternal antibodies in the egg. Using the common quail (Coturnix coturnix), we demonstrate a blocking effect of antigen specific maternal antibodies received via egg injection, on the offspring’s antibody response to an immune challenge with this antigen. Our results also suggest lower antibody production after a second immune challenge if maternal antibodies were present during first infection.4. Maternal antibodies thus have the potential to affect the development of offspring immunity. Our innovative technique will enable further work to manipulate the transfer of maternal antibodiesper sewhilst controlling for confounding effects linked to maternal vaccination. Because it also enables to manipulate the amount of antibodies transferred, it will facilitate further study of their adaptive value and ecological consequences in natural host‐parasite systems.
GATA‐binding protein‐3 regulates T helper type 2 cytokine and ifng loci through interaction with metastasis‐associated protein 2
Authors: Hwang Soo Seok , Lee Sumin , Lee Wonyong , Lee Gap Ryol 
Published: July 2010

SummaryGATA‐binding protein‐3 (GATA‐3) regulates the T helper type 2 (Th2) cytokine locus through induction of chromatin remodelling. However, the molecular mechanism for this is poorly understood. To understand this mechanism better, we screened GATA‐3 interacting proteins using affinity purification and mass spectrometry. We found that GATA‐3 bound to metastasis‐associated protein 2 (MTA‐2), a component of the NuRD chromatin remodelling complex. GATA‐3 and MTA‐2 in turn bound to several regulatory regions of the Th2 cytokine locus and the ifng promoter. Cell transfection assay showed that MTA‐2 acted as an antagonist with GATA‐3 in the expression of Th2 cytokines, but co‐operated with GATA‐3 in the repression of the ifng gene expression. These results suggest that GATA‐3 interacts with MTA‐2 to co‐ordinately regulate Th2 cytokine and ifng loci during T helper cell differentiation.
Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods
Authors: Owen Dylan M. , Gaus Katharina , Magee Anthony I. , Cebecauer Marek 
Published: July 2010

SummaryLipids and lipid domains are suggested to play an essential role in the heterogeneous organization of the plasma membrane in eukaryotic cells, including cells of the immune system. We summarize the results of advanced imaging and physical studies of membrane organization with special focus on the plasma membrane of lymphocytes. We provide a comprehensive up‐to‐date view on the existence of membrane lipid and protein clusters such as lipid rafts and suggest research directions to better understand these highly dynamic entities on the surface of immune cells.
Genetic contribution of the interferon gamma dinucleotide‐repeat polymorphism in South Indian women with endometriosis
Authors: Rozati Roya , Vanaja Maria Celestina , Nasaruddin Khaja 
Published: July 2010

AbstractAim:  To investigate whether the interferon‐γ (IFNG) gene dinucleotide (CA)‐repeat polymorphism is responsible in part for genetic susceptibility to endometriosis in South Indian women.Methods:  Following extraction of genomic DNA, genotyping of interferon‐γ CA‐repeat polymorphism was performed using genescan technology.Results:  The global IFNG allele frequencies in all patients with endometriosis were significantly different from those in the control women (χ2 = 37.062; 6 degrees of freedom; P ≤ 0.0001). Significant difference was observed in global allele frequencies between the control women and each clinical subgroup of patients with endometriosis except for patients suffering from endometriosis associated with adenomyosis. The difference was due to an increase in a12 (112 bp) allele in the patients with endometriosis and each clinical subgroup of patients with endometriosis.The distribution of the IFNG a12 genotypes was significantly different between patients with endometriosis and the control women. (χ2 = 10.635; 2 degrees of freedom; P = 0.0049). A significant difference in the IFNG a12 genotypes was found only among the three clinical subgroups.Conclusion:  These results suggest that the IFNG gene CA‐repeat polymorphism is associated with susceptibility to endometriosis in South Indian women.
Primary and bilateral tubal carcinoma is associated with long‐standing granulomatous inflammation and primary infertility: A case report
Authors: Muberra Kocak , Yasemin Tasci , Nurettin Boran , Zuhal Isikdogan , Berna Dilbaz 
Published: July 2010

AbstractWe report a case of bilateral and primary tubal carcinoma associated with granulomatous inflammation and long‐standing primary infertility. A 38‐year‐old woman with a 17‐year history of primary infertility presented with lower abdominal pain and granulomatous inflammation in the endometrial biopsy. Bilateral adnexal masses measuring 4 and 6 cm in size were detected and the serum cancer antigen 125 level was 141 IU/ml. Laparoscopic surgical exploration and frozen section on the resected tubes revealed bilateral fallopian tube adenocarcinoma and then a staging laparotomy was performed. Histopathological examination showed a primary bilateral Grade 2 tubal serous adenocarcinoma of Stage 1b associated with granulomatous salpingitis. Primary fallopian tube carcinomas in young women are extremely rare gynecological tumors that are infrequently diagnosed prior to surgical exploration and their cause is unknown. A definitive diagnosis could be made on the histopathological examination in our case with the evidence of chronic pelvic inflammation. Our findings suggest that chronic pelvic inflammation may play a role in carcinogenesis in the tubes of infertile women.
A stress survival response in retinal cells mediated through inhibition of the serine / threonine phosphatase PP2A
Authors: Finnegan Sorcha , Mackey Ashley M. , Cotter Thomas G. 
Published: July 2010

AbstractCell survival signalling involving the PI3K/Akt survival pathway can be negatively regulated by several phosphatases including PP2A. When retinal‐derived 661W cells were subjected to trophic factor deprivation this initiated a survival response through inhibition of the activity of PP2A and subsequent upregulation of the Erk and Akt survival pathways. We show this survival response via inhibition of PP2A activity was due in part to increased reactive oxygen species production when retinal cells were deprived of trophic factors. Inhibition of PP2A activity was mediated by a rapid and transient increase in phosphorylation at Tyr307, accompanied by an increase in demethylation and a decrease in the methylated form. Pre‐treatment withN‐acetyl‐l‐cysteine, which is involved in scavenging reactive oxygen species, prevented PP2A inhibition and subsequent upregulation of survival pathways. Pre‐treatment with the Src family kinase inhibitor PP2 resulted in approximately 50% reduction in cellular levels of phospho‐PP2A in trophic factor‐deprived 661W cells, suggesting an Src tyrosine kinase had a role to play in this redox regulation of cell survival. We observed similar events in the rd10 mouse retina where there was an increased survival response prior to retinal cell death mediated through an increase in both phospho‐PP2A and phospho‐Gsk. Together, these results demonstrate that when retinal cells are stressed there is an initial struggle to survive, mediated through inhibition of PP2A and subsequent upregulation of survival pathways, and that these events occur simultaneously with production of reactive oxygen species, thus suggesting an important cell‐signalling role for reactive oxygen species.
In vivo effects of morphine on neuronal fate and opioid receptor expression in zebrafish embryos
Authors: Sanchez‐Simon Fatima Macho , Arenzana Francisco J. , Rodriguez Raquel E. 
Published: July 2010

AbstractMorphine remains one of the most potent analgesic compounds used to control chronic pain despite its known adverse effects. It binds to the opioid receptors mu, delta and kappa, which are involved in aspects of neuronal fate such as cell proliferation, neuroprotection and neuronal differentiation. However, the effect of morphine on these processes is controversial and in vitro studies, as well as in vivo studies on adults and neonates in mammalian models, have not been able to clarify the diverse roles of morphine in the central nervous system. We have used zebrafish embryos to determine in vivo how morphine affects neuronal fate and opioid receptor gene expression and to elucidate if there is a link between these processes. Our results show that at 24 and 48 h post fertilization (hpf) morphine enhances cell proliferation, although it has opposing effects as an inducer of neuronal differentiation at these two stages, increasing the number of certain neuronal populations at 24 hpf and decreasing it at 48 hpf. The present study also demonstrates that in 24‐hpf embryos morphine acts as a neuroprotector against glutamate damage in motor neurons and Pax‐6‐positive neurons. Furthermore, the gene expression of the opioid receptors is altered by embryonic exposition to morphine. In conclusion, our study sheds new light on the in vivo roles of morphine, and it indicates for the first time that its implication in cell proliferation and neuroprotection might be related to changes in the gene expression of opioid receptors.
Group Leaders as Gatekeepers: Testing Safety Climate Variations across Levels of Analysis
Authors: Zohar Dov , Luria Gil 
Published: July 2010

This paper tests the moderating effect of transformational supervisory leadership on the relationship between organisational and group climates, using safety climate in risky operations as an exemplar. Results indicated that under low or poor organisational climate, indicative of limited organisational commitment to employee safety, transformational leaders promoted a higher group climate as compared to the organisational climate. Similarly, under a weak organisational climate, indicative of limited consensus among company employees regarding the priority of safety, transformational leaders promoted a stronger group climate, reflecting greater consensus among group members. This pattern suggests that supervisory leaders can act as gatekeepers, with transformational leaders offering better protection against potentially harmful organisation‐level priorities. Furthermore, transformational supervisors better informed their members of the organisational priorities as they perceived them, resulting in a stronger relationship between individual supervisors' perceptions and members' organisational climate perceptions. Implications for climate and leadership research are discussed.On s'intéresse dans cet article à l'impact régulateur du leadership hiérarchique de transformation sur la relation entre les climats organisationnels et de groupe en utilisant comme exemple la sécurité dans les opérations à risque. Les résultats montrent qu'en cas de climat organisationnel médiocre signifiant une implication organisationnelle minimale à propos de la sécurité du personnel, les leaders de transformation favorisent l'émergence d'un climat de groupe plus satisfaisant que le climat organisationnel. De même, si le climat organisationnel est fragile, traduisant un accord approximatif des salariés sur la prioritéà accorder à la sécurité, les leaders de transformation sont à l'origine d'un climat de groupe renforcé, expression d'un meilleur consensus parmi les membres du groupe. Ce schéma laisse penser que les leaders hiérarchiques peuvent intervenir comme gardiens, les leaders de transformation offrant une meilleure protection contre les priorités organisationnelles potentiellement nocives. De plus, les managers de transformation informent mieux leur équipe des priorités organisationnelles telles qu'ils les perçoivent, ce qui provoque une relation plus étroite entre les perceptions personnelles du manager et celles que son groupe a du climat organisationnel. On réfléchit aux retombées de ce travail sur les recherches portant sur le climat et le leadership.
Core Self‐Evaluations in Germany: Validation of a German Measure and its Relationships with Career Success
Authors: Stumpp Thorsten , Muck Peter M. , Hülsheger Ute R. , Judge Timothy A. , Maier Günter W. 
Published: July 2010

The present study reports the results of a validation of a German version of the Core Self‐Evaluations Scale (CSES) and its relationships with career success. Data were collected in three occupational samples to address various aspects of validation. Our results confirm the proposed one‐factor structure of the scale as well as convergent, discriminant, criterion, and predictive validity. Furthermore, the German CSES shows incremental validity over the individual core traits (neuroticism, self‐esteem, self‐efficacy, and locus of control), the Big Five, and positive and negative affect. Thus, the German version of the Core Self‐Evaluations Scale is a reliable, valid, and economic measure for both research and practical applications. Furthermore, hypothesised relationships of core self‐evaluations with objective as well as subjective career success were confirmed. Possible explanations of these relationships are discussed.On présente dans cet article une étude de validation d'une version allemande de la Core Self‐Evaluation Scale (CSES) avec son rapport au succès professionnel. Les données ont été recueillies auprès d'échantillons relevant de trois métiers pour couvrir plusieurs aspects de la validation. Nos résultats confirment la structure unifactorielle attendue ainsi que les validités convergente, discriminante, prédictive et critérielle. En outre, la CSES allemande présente une validité incrémentielle pour le Big Five, les émotions positives et négatives et les traits centraux individuels (névrotisme, estime de soi, auto‐efficience et locus of control). La version allemande de la CSES est par conséquent un outil fidèle, valide et économique aussi bien pour la recherche que pour les applications pratiques. Sans compter qu'ont été confirmées les relations supposées des auto‐évaluations centrales avec le succès professionnel, qu'il soit objectif ou subjectif. On analyse les raisons possibles de ces relations.
Values and Justice as Predictors of Perceived Stress in Lebanese Organisational Settings
Authors: Dbaibo Dania , Harb Charles , Van Meurs Nathalie 
Published: July 2010

Research investigating predictors of stress perceptions in organisational settings has been extensive. Value incongruence between employees and the organisation as well as organisational justice (distributive, procedural, informational, and interpersonal) are thought to be significant predictors of organisational outcomes. The current study investigated value incongruence and organisational justice as predictors of perceived stress in a sample of 362 organisational employees in Beirut, Lebanon. Results indicated that incongruence on value types of benevolence, power, and self‐direction are weakly predictive of perceived stress, while perceptions of interpersonal and distributive injustice are strongly predictive of perceived stress in the Lebanese organisational context. Implications of these differential findings for organisational and cross‐cultural research are discussed.Nombreuses ont été les recherches sur les prédicteurs du vécu du stress dans les organisations. La contradiction des valeurs des salariés et de l'organisation ainsi que la justice organisationnelle (distributive, procédurale, informationnelle et interpersonnelle) sont considérées comme étant des prédicteurs efficaces des réalités organisationnelles. Ce travail porte sur l'incohérence des valeurs et la justice organisationnelle en tant que prédicteurs du stress ressenti dans un échantillon de 362 salariés de Beyrouth (Liban). Les résultats montrent que des contradictions sur des valeurs comme la bienveillance, le pouvoir et l'autonomie sont peu prédictives du stress perçu, tandis que la perception des injustices interpersonnelle et distributive est fortement prédictive du stress ressenti dans le contexte organisationnel libanais. On discute des retombées de ces résultats différentiels sur les travaux organisationnels et transculturels.
Private and Public Incentives for Mergers in the Face of Foreign Entry
Authors: Fang Ryan , Richardson Martin 
Published: July 2010

AbstractWe consider private and public incentives for domestic firms to merge in the face of foreign entry. We consider the gains to two merging firms and to national welfare in a linear Cournot model. With heterogeneous firms and possible synergies, greater foreign entry tends to enhance both private and public incentives for domestic mergers. Thus, policymakers have no cause to doubt the intentions of firms seeking to merge: when it is in the firms' interests then it is also in the public interest. However, at least for certain parameterisations, private gains from mergers become positive at a lower level of foreign entry than do public gains. This suggests that private firms may have an incentive to overstate the degree of foreign competition they anticipate facing—for example, after liberalizing foreign investment rules—to persuade policymakers that a proposed domestic merger is in the national interest.
Facing the Challenge of the Rising Chinese Economy: ASEAN's Responses
Authors: Liu Yunhua , Ng Beoy Kui 
Published: July 2010

AbstractThe emergence of China as one of the largest trading nations in the world provides challenges and opportunities to its neighboring ASEAN countries. In the face of the rise of the Chinese economy, there were concerns that ASEAN economies may be adversely affected with the loss of competitiveness in the international market. One of the concerns is that the world export markets of labor‐intensive goods will be threatened if China turns into the world low‐cost manufacturing factory. Meanwhile, trade between China and ASEAN countries increased dramatically during the past decades. Not surprisingly, China's accession to the WTO and the future establishment of a free trade area (FTA) between ASEAN and China will further change the trade relations in the region. The paper first analyzes the past trade patterns between China and ASEAN countries and assesses the impact of the rising Chinese economy on ASEAN countries, in particular, the impacts on specific industries in each individual ASEAN country. Second, the paper examines the ever‐increasing role of foreign direct investment between the two regions and, finally, it analyzes and assess the policy responses of the ASEAN countries thus far and their possible consequences.