bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract A006: Bladder cancer risk stratification with the Oncuria 10 plex bead-based urinalysis assay using three different Luminex xMAP instrumentation platforms
Authors: Sakatani Toru , Tanaka Sunao , Murakami Kaoru , Waldron Richard , Hogrefe Wayne , Rosser Charles , Furuya Hideki 
Published: May 2024

Abstract
Introduction and Objectives Oncuria® is a bead-based multiplex fluorescence immunoassay that coordinately measures 10 protein biomarkers in urine samples. The current study compared assay performance and output when urine samples were evaluated with the Oncuria assay using three different fluorescence-analyzing instruments commonly used in diagnostic laboratories worldwide. Methods We compared the performance of the clinically validated Oncuria bladder cancer (BC) multiplex immunoassay when data output was generated on three different analyzer systems. Voided urine samples from 36 subjects (18 with BC and 18 Controls) were reacted with Oncuria test reagents in three 96 well microtiter plates, and consecutively evaluated on the LED/image based MagPix, and laser/flow based Luminex 200 and FlexMap 3D (all xMAP instruments from Luminex Corp., Austin, TX). The BC assay uses magnetic bead based fluorescence technology (xMAP, Multi-analyte profiling; Luminex) to simultaneously quantify 10 protein analytes in urine specimens [i.e., angiogenin (ANG), apolipoprotein E (ApoE), carbonic anhydrase IX (CA9), CXCL8/interleukin-8 (IL-8), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-10 (MMP-10), serpin A1/alpha-1 anti-trypsin (A1AT), serpin E1/plasminogen activator inhibitor-1 (PAI-1), CD138/syndecan-1 (SDC1), and vascular endothelial growth factor-A (VEGF-A)]. Results All three platforms categorized all 10 analytes in identical samples at nearly identical concentrations, with variance across systems typically &lt;5%. While the most contemporary instrument, the FlexMap 3D, output higher raw fluorescence values than the two comparator systems, standard curve slopes and analyte concentrations determined in urine samples were concordant across all three units. Forty-four percent of BC samples registered ≥1 analyte above the highest standard concentration, i.e., A1AT (n=7/18), IL-8 (n=5), and/or ANG (n=2). In Controls, A1AT was higher in one sample. Conclusions In conclusion, the Oncuria BC assay performed similarly well across three different flow analysis platforms for all 10 analytes simultaneously evaluated in urine samples. This agreement across instruments indicates that the test is amenable to standardized performance in laboratories using existing xMAP, without requiring costly outlays for new equipment.
Citation Format: Toru Sakatani, Sunao Tanaka, Kaoru Murakami, Richard Waldron, Wayne Hogrefe, Charles Rosser, Hideki Furuya. Bladder cancer risk stratification with the Oncuria 10 plex bead-based urinalysis assay using three different Luminex xMAP instrumentation platforms [abstract]. In: Proceedings of the AACR Special Conference on Bladder Cancer: Transforming the Field; 2024 May 17-20; Charlotte, NC. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(10_Suppl):Abstract nr A006.
Abstract A019: TRIM29 enhances the invasion of bladder cancer cells by modulating the intermediate filament network and focal adhesions
Authors: Wang Yin , Jerome Nicole A. , Kelleher Allan , Henderson Marian L. , Day Mark , Coulombe Pierre A. , Palmbos Phillip L. 
Published: May 2024

Abstract
Background: Bladder cancer patient treatment and outcomes are determined by the tumor’s ability to invade and metastasize. Our previous work has shown that TRIM29, also known as ATDC, is regulated by TP63 in basal bladder cancers, where it promotes cancer invasion and metastasis. We also observe that TRIM29 predominately localizes to intermediate filaments in invasive bladder cancer cells. Hypothesis: TRIM29 promotes the invasive progression of bladder cancer by regulating K14-containing intermediate filaments and focal adhesions. Methods: To test this hypothesis, we utilized cell culture models of bladder cancer, live cell imaging and 2D and 3D invasion assays to examine the role of TRIM29 in the regulation of K14, focal adhesions and the migration and invasion and bladder cancer cells. Results: We observed a physical interaction between TRIM29 and K14 filamentous structures in bladder cancer cells. We also found that TRIM29 and K14 regulate focal adhesion stability during bladder cancer invasion. Finally, we found that both K14 and the focal adhesion protein, zyxin (ZYX), are essential for bladder cancer migration and invasion, acting downstream of TRIM29. Conclusions: Our findings highlight a novel role for TRIM29 in regulating the cytoskeleton and focal adhesion dynamics during tumor cell invasion and suggest a potential therapeutic pathway for treating bladder cancer.
Citation Format: Yin Wang, Nicole A. Jerome, Allan Kelleher, Marian L. Henderson, Mark Day, Pierre A. Coulombe, Phillip L. Palmbos. TRIM29 enhances the invasion of bladder cancer cells by modulating the intermediate filament network and focal adhesions [abstract]. In: Proceedings of the AACR Special Conference on Bladder Cancer: Transforming the Field; 2024 May 17-20; Charlotte, NC. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(10_Suppl):Abstract nr A019.
Abstract IA005: Y chromosome loss in cancer drives growth by evasion of adaptive immunity
Authors: Theodorescu Dan 
Published: May 2024

Abstract
Loss of the Y chromosome (LOY) is observed in 10-40% of bladder cancers, but its clinical and biological significance is unknown. We will present genomic and transcriptomic studies that show LOY correlates with poor prognoses in patients. We performed studies of murine bladder cancer cells with LOY as well as those with deletion of Y chromosome by CRISPR/Cas9 and compared them to cells with and intact Y chromosome as controls. Y+ and Y- tumors grew similarly in vitro, while Y- tumors grew faster than Y+ tumors in immune competent hosts in a T cell-dependent manner. Flow cytometric analyses demonstrated that Y- tumors promote exhaustion of CD8+ T cells in the tumor microenvironment. These findings were supported by data obtained from single-nuclei RNA sequencing and spatial proteomic evaluation of human bladder cancers. Compared to Y+ tumors, Y- tumors exhibited a superior response to anti-PD-1 therapy in mice. In bladder cancer patients those with LOY tumors have better prognosis than those with Y+ tumors. This is the first evidence demonstrating that LOY in tumors alters T cell function by promoting T cell exhaustion and sensitizes them to PD-1-targeted immunotherapy. This work provides novel insights into basic LOY biology as well as the potential for the development of biomarkers for improved cancer immunotherapy.
Citation Format: Dan Theodorescu. Y chromosome loss in cancer drives growth by evasion of adaptive immunity [abstract]. In: Proceedings of the AACR Special Conference on Bladder Cancer: Transforming the Field; 2024 May 17-20; Charlotte, NC. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(10_Suppl):Abstract nr IA005.
Abstract IA019: Lessons from glioblastoma diversity, heterogeneity, and beginnings
Authors: Parada Luis F. 
Published: May 2024

Abstract
Glioblastoma (GBM) is a dire disease with poor prognosis and survival outcomes have changed little over the past two decades. Various genomic studies have emphasized the heterogeneity of GBM yet a consensus on the causes and its role in tumor recurrence has not been reached. Multiple studies using genetic and patient-derived xenograft (PDX) mouse models have demonstrated an inverse relationship between differentiation state and tumor initiation efficiency with neural stem and progenitor cells as the most efficient. However, other studies have proposed that well differentiated cells like neurons and astrocytes can form tumors. In addition, bioinformatic analyses of human primary GBM have reported that plasticity allows for mobility among cell states exist. Regardless of transcriptional modulation in GBM cells, to achieve progress in understanding and addressing clinical recurrence, the outstanding goal remains to identify and isolate the GBM cell type(s) responsible for therapy resistance and recurrence. To achieve this, we have employer autochthonous GBM models as well as orthotopic patient derived xenografts to in parallel examine tumor heterogeneity, cell of origin, and functional consequences.
Citation Format: Luis F. Parada. Lessons from glioblastoma diversity, heterogeneity, and beginnings [abstract]. In: Proceedings of the AACR Special Conference on Bladder Cancer: Transforming the Field; 2024 May 17-20; Charlotte, NC. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(10_Suppl):Abstract nr IA019.
Left Atrial Mechanics Following Preeclamptic Pregnancy
Authors: O’Driscoll Jamie M. , McCarthy Fergus P. , Giorgione Veronica , Jalaludeen Navazh , Seed Paul T. , Gill Carolyn , Sparkes Jenie , Poston Lucilla , Marber Mike , Shennan Andrew H. , Chappell Lucy C. , Thilaganathan Basky , Leeson Paul 
Published: May 2024

BACKGROUND:
Preterm preeclampsia is a pregnancy complication associated with myocardial dysfunction and premature cardiovascular disease morbidity and mortality. Left atrial (LA) strain is a noninvasive index of left ventricular end diastolic pressure and an early marker of heart failure risk. This study aimed to evaluate LA strain during the postpartum period in participants with and without preterm preeclampsia and to assess whether this varied in the presence of hypertension, cardiac dysfunction or both.

METHODS:
In this longitudinal cohort study, 321 women from 28 hospitals with preterm preeclampsia (cases) underwent cardiovascular assessment 6 months postpartum. This is a secondary analysis of the PHOEBE study (ISRCTN01879376). An uncomplicated pregnancy control group (n=30) was recruited from a single center for comparison. A full cross-sectional transthoracic echocardiogram was performed, and from these images, the myocardial strain of the left atrium, including reservoir, conduit, and contractile strain, as well as LA stiffness, were calculated.

RESULTS:

At 6 months postpartum, compared with controls, prior preeclampsia was associated with a significantly attenuated LA reservoir, conduit, and contractile strain, as well as increased LA stiffness (all
P
<0.001). LA strain was further reduced in preeclamptic women who had and had not developed hypertension, systolic, or diastolic dysfunction at 6 months postpartum (all
P
<0.05).

CONCLUSIONS:
LA mechanics were significantly attenuated at 6 months postpartum in participants with preterm preeclampsia, whether or not they remained hypertensive or had evidence of ventricular dysfunction. Further studies are needed to determine whether postnatal LA strain may identify women at greater risk for future cardiovascular disease.
Comprehensive Cardiovascular Risk Factor Control With a Mobile Health Cardiovascular Risk Self‐Management Program
Authors: Paz Edo , Pargaonkar Vedant S. , Roach Brian J. , Meadows Morgan , Roberts Jennifer M. , Gazit Tomer , Zaleski Amanda L. , Craig Kelly Jean Thomas , Serra Steven J. , Dunn Pat , Michos Erin D. 
Published: May 2024

Background
Mobile health technology's impact on cardiovascular risk factor control is not fully understood. This study evaluates the association between interaction with a mobile health application and change in cardiovascular risk factors.

Methods and Results

Participants with hypertension with or without dyslipidemia enrolled in a workplace‐deployed mobile health application‐based cardiovascular risk self‐management program between January 2018 and December 2022. Retrospective evaluation explored the influence of application engagement on change in blood pressure (BP), total cholesterol (TC), low‐density lipoprotein cholesterol (LDL‐C), and weight. Multiple regression analyses examined the influence of guideline‐based, nonpharmacological lifestyle‐based digital coaching on outcomes adjusting for confounders. Of 102 475 participants, 49.1% were women. Median age was 53 (interquartile range, 43–61) years, BP was 134 (interquartile range, 124–144)/84 (interquartile range, 78–91) mm Hg, TC was 183 (interquartile range, 155–212) mg/dL, LDL‐C was 106 (82–131) mg/dL, and body mass index was 30 (26–35) kg/m
2
. At 2 years, participants with baseline systolic BP ≥140 mm Hg reduced systolic BP by 18.6 (SEM, 0.3) mm Hg. At follow up, participants with baseline TC ≥240 mg/dL reduced TC by 65.7 (SEM, 4.6) mg/dL, participants with baseline LDL‐C≥160 mg/dL reduced LDL‐C by 66.6 (SEM, 6.2) mg/dL, and participants with baseline body mass index ≥30 kg/m
2
lost 12.0 (SEM, 0.3) pounds, or 5.1% of body weight. Interaction with digital coaching was associated with greater reduction in all outcomes.

Conclusions
A mobile health application‐based cardiovascular risk self‐management program was associated with favorable reductions in BP, TC, LDL‐C, and weight, highlighting the potential use of this technology in comprehensive cardiovascular risk factor control.
Prospective Validation of Glial Fibrillary Acidic Protein,
d
‐Dimer, and Clinical Scales for Acute Large‐Vessel Occlusion Ischemic Stroke Detection
Authors: Durrani Yasir , Gerstl Jakob V. E. , Murphy Danielle , Harris Ashley , Saali Imane , Gropen Toby , Shekhar Shashank , Kappel Ari D. , Patel Nirav J. , Du Rose , Guardia Rodolfo E. Alcedo , Vicenty‐Padilla Juan C. , Dmytriw Adam A. , Pereira Vitor Mendes , Izzy Saef , Khan Allauddin , Aziz‐Sultan Mohammed A. , Liebeskind David S. , Davies Jason M. , Siddiqui Adnan H. , Gaude Edoardo , Bernstock Joshua D. 
Published: May 2024

Background

Large‐vessel occlusion (LVO) ischemic stroke is responsible for significant morbidity and mortality. We have previously described a novel tool for acute LVO detection that combines blood‐based biomarkers (glial fibrillary acidic protein and
d
‐dimer) with stroke severity scales to achieve high accuracy. Accordingly, the present study sought to prospectively validate cutoff values that we had previously established for biomarkers and scales.

Methods

The TIME (Testing for Identification Markers of Stroke) trial was designed as a prospective observational diagnostic accuracy study. All ambulance‐identified stroke code activations <18 hours from symptom onset were recruited at Brandon Regional Hospital (Brandon, FL) between May 2021 and August 2022. Previously determined cutoff concentrations of plasma glial fibrillary acidic protein (213 pg/mL) and
d
‐dimer (600 ng/mL) were used in combination with prehospital stroke scales to detect LVO. We compared rates of LVO detection against a reference standard using computed tomography/magnetic resonance angiography.

Results

A total of 382 patients with suspected stroke were recruited. The final cohort was composed of 323 patients with suspected stroke with the following distribution: LVO ischemic stroke (n = 29, 9%), non‐LVO ischemic stroke (n = 48, 15%), hemorrhagic stroke (n = 13, 4%), transient ischemic attack (n = 12, 3.9%), and stroke mimics (n = 220, 68.1%). Combining blood‐based biomarkers (glial fibrillary acidic protein and
d
‐dimer) with the scale field assessment stroke triage for emergency destination yielded the best performance for LVO detection, with specificity of 94% and sensitivity of 71%. Performance was found to be higher in a subanalysis focusing on patients presenting <6 hours from symptom onset, with 93% specificity and 81% sensitivity. Critically, application of the biomarker and stroke scale algorithms ruled out all patients with hemorrhage.

Conclusion

The present work prospectively validated the potential utility of previously defined glial fibrillary acidic protein and
d
‐dimer cutoff levels (ie, 213 pg/mL and 600 ng/mL, respectively), demonstrating their value for discrimination of LVO stroke from differential diagnoses during code stroke workups. (ClinicalTrials.gov number, NCT04292600.)
Blasphemy! Levinas and the Unjustification of Suffering
Authors: Severson Eric R. 
Published: May 2024

Abstract
In this article, Severson explores the insights of Emmanuel Levinas regarding theodicy and the problem of evil. Levinas considers philosophical and practical justifications of suffering to be blasphemous, violating the sanctity of the suffering of the other person, even when well-meant. Severson introduces distinctions between “pain” and “suffering” to extrapolate and explain Levinas’s striking rejection of theodicy.
The Use of English Pragmatic Markers by Learners of English from Different Linguacultural Backgrounds
Authors: Pan Zhaoyi 
Published: May 2024

Abstract
This study compared the uses of the textual and interpersonal functions of English pragmatic markers (PMs) by learners of English as a foreign language (EFL) from three different linguacultural backgrounds in two spoken genres. Thirty Chinese, 30 Thai and 30 Indonesian EFL learners were recruited to participate in semi-structured interviews and dyadic conversations. Significant differences in the ways in which the participants used textual and interpersonal functions in the two spoken genres were found, whereas there were significant differences in the use of textual functions by speakers from different linguacultural backgrounds. The spoken genres and the linguacultural backgrounds mainly affected the more frequently used functions, and had no effect on the functions that were used less frequently. Furthermore, the spoken genre had a greater effect on the different uses of the functions of English PMs than did the linguacultural backgrounds.
Moving beyond the Aerosol Climatology of WRF-Solar: A Case Study over the North China Plain
Authors: Wang Wenting , Shi Hongrong , Fu Disong , Liu Mengqi , Li Jiawei , Shan Yunpeng , Hong Tao , Yang Dazhi , Xia Xiang’ao 
Published: May 2024

Abstract
Numerical weather prediction (NWP), when accessible, is a crucial input to short-term solar power forecasting. WRF-Solar, the first NWP model specifically designed for solar energy applications, has shown promising predictive capability. Nevertheless, few attempts have been made to investigate its performance under high aerosol loading, which attenuates incoming radiation significantly. The North China Plain is a polluted region due to industrialization, which constitutes a proper testbed for such investigation. In this paper, aerosol direct radiative effect (DRE) on three surface shortwave radiation components (i.e., global, beam, and diffuse) during five heavy pollution episodes is studied within the WRF-Solar framework. Results show that WRF-Solar overestimates instantaneous beam radiation up to 795.3 W m−2 when the aerosol DRE is not considered. Although such overestimation can be partially offset by an underestimation of the diffuse radiation of about 194.5 W m−2, the overestimation of the global radiation still reaches 160.2 W m−2. This undesirable bias can be reduced when WRF-Solar is powered by Copernicus Atmosphere Monitoring Service (CAMS) aerosol forecasts, which then translates to accuracy improvements in photovoltaic (PV) power forecasts. This work also compares the forecast performance of the CAMS-powered WRF-Solar with that of the European Centre for Medium-Range Weather Forecasts model. Under high aerosol loading conditions, the irradiance forecast accuracy generated by WRF-Solar increased by 53.2% and the PV power forecast accuracy increased by 6.8%.

Significance Statement
Numerical weather prediction (NWP) is the “go-to” approach for achieving high-performance day-ahead solar power forecasting. Integrating time-varying aerosol forecasts into NWP models effectively captures aerosol direct radiation effects, thereby enhancing the accuracy of solar irradiance forecasts in heavily polluted regions. This work not only quantifies the aerosol effects on global, beam, and diffuse irradiance but also reveals the physical mechanisms of irradiance-to-power conversion by constructing a model chain. Using the North China Plain as a testbed, the performance of WRF-Solar on solar power forecasting on five severe pollution days is analyzed. This version of WRF-Solar can outperform the European Centre for Medium-Range Weather Forecasts model, confirming the need for generating high spatial–temporal NWP.
Extracellular matrix marker LAMC2 targets ZEB1 to promote TNBC malignancy via up-regulating CD44/STAT3 signaling pathway
Authors: Wang Ding , Keyoumu Kailibinuer , Yu Rongji , Wen Doudou , Jiang Hao , Liu Xinchun , Di Xiaotang , Zhang Shubing 
Published: May 2024

Abstract
Background
Triple negative breast cancer (TNBC) is a heterogeneous and aggressive disease characterized by a high risk of mortality and poor prognosis. It has been reported that Laminin γ2 (LAMC2) is highly expressed in a variety of tumors, and its high expression is correlated with cancer development and progression. However, the function and mechanism by which LAMC2 influences TNBC remain unclear.

Methods
Kaplan–Meier survival analysis and Immunohistochemical (IHC) staining were used to examine the expression level of LAMC2 in TNBC. Subsequently, cell viability assay, wound healing and transwell assay were performed to detect the function of LAMC2 in cell proliferation and migration. A xenograft mouse model was used to assess tumorigenic function of LAMC2 in vivo. Luciferase reporter assay and western blot were performed to unravel the underlying mechanism.

Results
In this study, we found that higher expression of LAMC2 significantly correlated with poor survival in the TNBC cohort. Functional characterization showed that LAMC2 promoted cell proliferation and migration capacity of TNBC cell lines via up-regulating CD44. Moreover, LAMC2 exerted oncogenic roles in TNBC through modulating the expression of epithelial-mesenchymal transition (EMT) markers. Luciferase reporter assay verified that LAMC2 targeted ZEB1 to promote its transcription. Interestingly, LAMC2 regulated cell migration in TNBC via STAT3 signaling pathway.

Conclusion
LAMC2 targeted ZEB1 via activating CD44/STAT3 signaling pathway to promote TNBC proliferation and migration, suggesting that LAMC2 could be a potential therapeutic target in TNBC patients.