bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal and regional expression changes and co‐staining patterns of metabolic and stemness‐related markers during glioblastoma progression
Authors: Kubelt Carolin , Gilles Lea , Hellmold Dana , Blumenbecker Tjorven , Peschke Eva , Will Olga , Ahmeti Hajrullah , Hövener Jan‐Bernd , Jansen Olav , Lucius Ralph , Synowitz Michael , Held‐Feindt Janka 
Published: May 2024

AbstractGlioblastomas (GBMs) are characterized by high heterogeneity, involving diverse cell types, including those with stem‐like features contributing to GBM's malignancy. Moreover, metabolic alterations promote growth and therapeutic resistance of GBM. Depending on the metabolic state, antimetabolic treatments could be an effective strategy. Against this background, we investigated temporal and regional expression changes and co‐staining patterns of selected metabolic markers [pyruvate kinase muscle isozyme 1/2 (PKM1/2), glucose transporter 1 (GLUT1), monocarboxylate transporter 1/4 (MCT1/4)] in a rodent model and patient‐derived samples of GBM. To understand the cellular sources of marker expression, we also examined the connection of metabolic markers to markers related to stemness [Nestin, Krüppel‐like factor 4 (KLF4)] in a regional and temporal context. Rat tumour biopsies revealed a temporally increasing expression of GLUT1, higher expression of MCT1/4, Nestin and KLF4, and lower expression of PKM1 compared to the contralateral hemisphere. Patient‐derived tumours showed a higher expression of PKM2 and Nestin in the tumour centre vs. edge. Whereas rare co‐staining of GLUT1/Nestin was found in tumour biopsies, PKM1/2 and MCT1/4 showed a more distinct co‐staining with Nestin in rats and humans. KLF4 was mainly co‐stained with GLUT1, MCT1 and PKM1/2 in rat and human tumours. All metabolic markers yielded individual co‐staining patterns among themselves. Co‐staining mainly occurred later in tumour progression and was more pronounced in tumour centres. Also, positive correlations were found amongst markers that showed co‐staining. Our results highlight a link between metabolic alterations and stemness in GBM progression, with complex distinctions depending on studied markers, time points and regions.
Causal association between circulating inflammatory cytokines and intracranial aneurysm and subarachnoid hemorrhage
Authors: He Qiang , Wang Wenjing , Xiong Yang , Tao Chuanyuan , Ma Lu , You Chao 
Published: May 2024

AbstractBackground and purposeThe causal association between inflammatory cytokines and the development of intracranial aneurysm (IA), unruptured IA (uIA) and subarachnoid hemorrhage (SAH) lacks clarity.MethodsThe summary‐level datasets for inflammatory cytokines were extracted from a genome‐wide association study of the Finnish Cardiovascular Risk in Young Adults Study and the FINRISK survey. The summary statistics datasets related to IA, uIA and SAH were obtained from the genome‐wide association study meta‐analysis of the International Stroke Genetics Consortium and FinnGen Consortium. The primary method employed for analysis was inverse variance weighting (false discovery rate), supplemented by sensitivity analyses to address pleiotropy and enhance robustness.ResultsIn the International Stroke Genetics Consortium, 10, six and eight inflammatory cytokines exhibited a causal association with IA, uIA and SAH, respectively (false discovery rate, p < 0.05). In FinnGen datasets, macrophage Inflammatory Protein‐1 Alpha (MIP_1A), MIP_1A and interferon γ‐induced protein 10 (IP_10) were verified for IA, uIA and SAH, respectively. In the reverse Mendelian randomization analysis, the common cytokines altered by uIA and SAH were vascular endothelial growth factor (VEGF), MIP_1A, IL_9, IL_10 and IL_17, respectively. The meta‐analysis results show that MIP_1A and IP_10 could be associated with the decreased risk of IA, and MIP_1A and IP_10 were associated with the decreased risk of uIA and SAH, respectively. Notably, the levels of VEGF, MIP_1A, IL_9, IL_10 and TNF_A were increased with uIA. Comprehensive heterogeneity and pleiotropy analyses confirmed the robustness of these results.ConclusionOur study unveils a bidirectional association between inflammatory cytokines and IA, uIA and SAH. Further investigations are essential to validate their relationship and elucidate the underlying mechanisms.
Rapid identification of Ripersiella (= Rhizoecus) hibisci (Kawai & Takagi, 1971) (Hemiptera: Rhizoecidae) with TaqMan probe‐based real‐time PCR
Authors: Rizzo D. , Moriconi M. , Carli M. , Stabile I. , Zubieta C. G. , Marrucci A. , Ranaldi C. , Palmigiano B. , d'Agostino A. , Guastini M. , Gilli G. , Bartolini L. , Miele F. , Bernardo U. , Rossi E. 
Published: May 2024

AbstractRipersiella hibisci (Kawai & Takagi, 1971) is a root mealybug included in the EPPO A1 list of pests recommended for regulation and an EU A1 Quarantine pest list (Annex IIA). This pest causes damage to several species of monocotyledonous and dicotyledonous ornamental and foliage potted plants, especially bonsais. The underground location of R. hibisci makes its detection difficult during phytosanitary controls, and moreover, it can be confused with other non‐regulated species of mealybugs (e.g. Planococcus citri, P. ficus, Rhizoecus falcifer, Rhizoecus dianthi). Thus, a molecular test based on real‐time PCR was designed for rapid and unambiguous identification of this mealybug. Analytical sensitivity, analytical specificity, repeatability and reproducibility of the test were assessed as part of the validation. The test showed 100% analytical specificity (exclusivity and inclusivity), allowing the target to be distinguished from all non‐target species tested (exclusivity); the inclusivity was demonstrated, validating the test on different populations of the pest. The molecular test is an efficient tool for the identification of the root mealybug R. hibisci (in its various life stages), which is particularly useful when a rapid screening of samples is needed to limit or avoid the introduction of the pest.
The histological spectrum and immunoprofile of head and neck NUT carcinoma: A multicentre series of 30 cases
Authors: Viswanathan Kartik , Hahn Elan , Dogan Snjezana , Weinreb Ilan , Dickson Brendan C , MacMillan Christina , Katabi Nora , Magliocca Kelly , Ghossein Ronald , Xu Bin 
Published: May 2024

Background and aimHead and neck nuclear protein of testis carcinoma (HN‐NUT) is a rare form of carcinoma diagnosed by NUT immunohistochemistry positivity and/or NUTM1 translocation. Although the prototype of HN‐NUT is a primitive undifferentiated round cell tumour (URC) with immunopositivity for squamous markers, it is our observation that it may assume variant histology or immunoprofile.MethodsWe conducted a detailed clinicopathological review of a large retrospective cohort of 30 HN‐NUT, aiming to expand its histological and immunohistochemical spectrum.ResultsThe median age of patients with HN‐NUT was 39 years (range = 17–86). It affected the sinonasal tract (43%), major salivary glands (20%), thyroid (13%), oral cavity (7%), larynx (7%), neck (7%) and nasopharynx (3%). Although most cases of HN‐NUT (63%) contained a component of primitive URC tumour, 53% showed other histological features and 37% lacked a URC component altogether. Variant histological features included basaloid (33%), differentiated squamous/squamoid (37%), clear cell changes (13%), glandular differentiation (7%) and papillary architecture (10%), which could co‐exist. While most HN‐NUT were positive for keratins, p63 and p40, occasional cases (5–9%) were entirely negative. Immunopositivity for neuroendocrine markers and thyroid transcription factor‐1 was observed in 33 and 36% of cases, respectively. The outcome of HN‐NUT was dismal, with a 3‐year disease specific survival of 38%.ConclusionsHN‐NUT can affect individuals across a wide age range and arise from various head and neck sites. It exhibits a diverse spectrum of histological features and may be positive for neuroendocrine markers, potentially leading to underdiagnosis. A low threshold to perform NUT‐specific tests is necessary to accurately diagnose HN‐NUT.
Characteristics of mountain vs. lowland dairy products
Authors: Ungureanu‐Iuga Mădălina , Surdu Ioan , Necula Doru 
Published: May 2024

SummaryBesides the clean environment, mountain pastures are important sources of bioactive compounds that contribute to the quality of dairy products resulting from animals grazing there. This review aimed to compare the main differences among mountain and lowland dairies in terms of chemical composition, physical and sensory properties and microflora. The most important factors influencing final product properties are the biodiversity of pastures and the abundance of plants in terpenes and fatty acids, farm practices, animal breeds and climatic conditions. Differences between dairies from lowland and mountain products were observed regarding the sensory profile, microflora and nutritional and functional value. The comparisons of the results presented in the literature concluded that some mountain dairies presented more than 50% higher content of terpenes compared to lowland products. Furthermore, the amounts of polyunsaturated fatty acids (PUFA) were more than 15% greater in mountain products compared to lowland ones, conjugated linoleic acids more than 35%, n−6 and n−3 PUFA more than 10%, while the saturated fatty acids content was reported to be more than 3% lower in mountain dairies. In conclusion, mountain dairy products can be distinguished from lowland ones, especially by their content of terpenes and fatty acid profile, along with the microflora which are influenced by livestock management.
Christ the Mediator and Head of Angels in Calvin's Theology
Authors: Rankin Arthur 
Published: May 2024

AbstractThough Calvin is averse to theological speculation, he is the first to claim that Christ is Mediator and Head of Angels. This often‐overlooked office is present consistently throughout the various editions of the Institutes and can be found in his treatises, commentaries, catechism, and various sermons. This paper argues that Christ's mediation of angels is a form of ontological mediation that serves as the logical foundation for his redemptive mediation of the Elect. Sin is an intensification of a preexisting problem that requires the Son to be the medius and Mediator between God and creation because of his unique position in the Trinitarian taxis as the begotten one. This overlooked office places Calvin's theology of mediation in a greater cosmic context where the triune God is uniting all things to himself typified in the angels and the Elect.
Understanding the mechanism of safety attitude mitigates the turnover intention novice nurses via the person‐centred method: A theory‐driven, deductive cross‐sectional study
Authors: Zheng Xutong , Liu Huan , Zhang Man , Yang Zhen , Dong Yong , Qin Zhuzhu , Tao Xiubin , Zhang Xiancui , Wang Aiping 
Published: May 2024

AbstractAimExamine profiles of safety attitudes among novices and explore whether profiles moderate the occupational identity–turnover pathway.BackgroundNovice nurses face unique challenges in adopting positive safety attitudes, which influence outcomes like turnover. However, past research found only average levels of safety attitudes among novices, ignoring possible heterogeneity. Exploring whether meaningful subgroups exist based on safety perspectives and factors shaping them can provide insights to improve safety attitudes and retention.DesignThis study was designed as a cross‐sectional investigation.MethodsData were collected through the distribution of questionnaires. Descriptive statistics were first conducted, followed by latent profile analysis. We then carried out univariate analysis and ordinal multinomial regression to explore the factors shaping the different profiles. Finally, we examine the moderating effect of nurses' safety attitudes with different latent profiles on the relationship between professional identification and turnover intention.ResultsA total of 816 novice nurses were included. Three profiles were identified: high, moderate and low safety attitudes – higher attitudes were associated with lower turnover intention. Interest in nursing, health status, identity and turnover predicted profile membership. Moderate profile had a stronger buffering effect on the identity–turnover link versus high profile.ConclusionMultiple safety attitude profiles exist among novice nurses. Certain factors like interest in nursing and occupational identity are associated with more positive safety profiles. Targeting these factors could potentially improve safety attitudes and reduce turnover among novice nurses. The moderating effects suggest that tailored interventions matching specific subgroups may maximize impact.ImpactAssessing subgroup attitudes enables tailored training for novices' specific needs, nurturing continuous improvement. Supporting early career development and role identity may strengthen retention intentions.
Location and caller familiarity influence mobbing behaviour and the likely ecological impact of noisy miners around colony edges
Authors: Kern Julie M. , McDonald Paul G. 
Published: May 2024

Mobbing is a widespread, vocally coordinated behaviour where species approach and harass a threat. The noisy miner (Manorina melanocephala) is a notorious native Australian honeyeater, well‐known for its hyperaggressive mobbing. Numerous studies have identified negative impacts of their mobbing behaviour, highlighting the exclusion of competitors from colony areas and the resulting loss of woodland‐bird biodiversity. Despite this, few studies have investigated mobbing itself, and our understanding of the factors which influence its expression remains limited. Here, we use a field‐based playback experiment to investigate whether mobbing responses vary in relation to colony borders and caller familiarity. Noisy miners were more likely to respond, reacted more quickly and responded more strongly to mobbing calls broadcast inside as opposed to outside the colony. These behavioural differences likely arise from variation in the relative costs and benefits of responding. When noisy miners did mob outside the colony, more individuals joined in response to unfamiliar as opposed to familiar callers. Our results reveal that noisy miner mobbing may not be as indiscriminate as often assumed, with caller familiarity and location influencing this behaviour. We suggest there are benefits to greater consideration of the factors impacting noisy miner mobbing behaviour.Keywords: Avian biodiversity, interspecific aggression, mobbing behaviour, noisy miner, vocal communication
Brexit Rhapsody: Exploring Patterns of Issue Salience in the Negotiations
Authors: Moloney David , Jensen Mads Dagnis 
Published: May 2024

AbstractThis study revisits the salience of the Brexit negotiations using an advanced Decision‐Making in the European Union (EU) methodology to assess the relative importance of 20 key issues amongst the EU27 member states, the European Commission and the UK. Unlike earlier studies that suggest more uniform salience levels, except for the UK, our analysis identifies eight clusters of actors influenced by their political, economic and geographical contexts. These differences in salience levels have been conducive to logrolling, which likely supported a unified EU stance and a successful agreement with the UK. Additionally, our research quantitatively confirms the UK's distinct salience position, highlighting its isolation and reducing its ability to use divisive negotiation tactics. These findings offer insights into both the dynamics of past Brexit negotiations and ongoing EU–UK policy developments. They contribute to the analysis of Brexit and international negotiations in general by systematically exploring salience in high‐level diplomatic negotiations.
Development of real‐time recombinase polymerase amplification (RPA) and RPA combined with lateral flow dipstick (LFD) assays for the rapid and sensitive detection of cyprinid herpesvirus 3
Authors: Li Yingying , Li Ruifan , Mo Xubing , Wang Yingying , Yin Jiyuan , Bergmann Sven M. , Ren Yan , Pan Houjun , Shi Cunbin , Zhang Defeng , Wang Qing 
Published: May 2024

AbstractIn this issue, we established rapid, cost‐effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA‐LFD) and real‐time RPA for cyprinid herpesvirus 3(CyHV‐3), and evaluated their sensitivity, specificity, and applicability, the real‐time RPA method could achieve sensitive diagnosis of CyHV‐3 within 1.3 copies per reaction, respectively. The real‐time RPA method is 10‐fold more sensitive than RPA‐LFD method. The exact number of CyHV‐3 can be calculated in each sample by real‐time RPA. The sera from koi also can be tested in these methods. In addition, no cross‐reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV‐1), cyprinid herpesvirus 2(CyHV‐2), type I grass carp reovirus (GCRV‐I), type II GCRV (GCRV‐II), type III GCRV (GCRV‐III), and Aeromonas hydrophila.
The impact of base design and restoration type on the resin consumption, trueness, and dimensional stability of dental casts additively manufactured from liquid crystal display 3D printers
Authors: Tseng Chih‐Wei , Lin Wei‐Shao , Sahrir Citra Dewi , Lin Wei‐Chun 
Published: May 2024

AbstractPurposeTo evaluate the effects of two base types and three restoration designs on the resin consumption and trueness of the 3D‐printed dental casts. Additionally, the study explored the dimensional stability of these 3D‐printed dental casts after 1 year of storage.Materials and methodsVarious types of reference dental casts were specifically designed to represent three types of dental restoration fabrications, including full‐arch (FA), long‐span (LS), and single‐unit (SU) prostheses. The reference casts were digitized with a dental laboratory scanner and used to create flat and hollow base designs (N = 18) for the 3D‐printed study casts. The 3D‐printed study casts were digitized and evaluated against their corresponding references immediately after 3D printing and again after 1 year of storage, with the trueness quantified using the root mean square error (RMSE) at both time points. Volumes of resin used were recorded to measure resin consumption, and the weights of the 3D‐printed study casts were also measured. The data were analyzed using two‐way ANOVA and a Tukey post hoc test, α = 0.05.ResultsVolumetric analysis showed the flat‐base design had significantly higher resin consumption with weights for the FA group at 42.51 ± 0.16 g, the LS group at 31.64 ± 0.07 g, and the SU group at 27.67 ± 0.31 g, as opposed to 26.22 ± 1.01 g, 22.86 ± 0.93 g, and 20.10 ± 0.19 g for the hollow designs respectively (p < 0.001). Trueness, assessed through two‐way ANOVA, revealed that the flat‐base design had lower RMSE values indicating better trueness in the LS (54 ± 6 µm) and SU (59 ± 7 µm) groups compared to the hollow‐base design (LS: 73 ± 5, SU: 99 ± 11 µm, both p < 0.001), with no significant difference in the FA group (flat‐base: 50 ± 3, hollow: 47 ± 5 µm, p = 0.398). After 1 year, the flat‐base design demonstrated superior dimensional stability in the LS (flat base: 56 ± 6 µm, hollow base: 149 ±45 µm, p < 0.001) and SU groups (flat base: 95 ± 8 µm, hollow base: 183 ±27 µm, p < 0.001), with the FA group showing no significant difference in the base design (flat base: 47 ± 9, hollow base: 62 ± 12 µm, p = 0.428).ConclusionsThe hollow‐base design group showed lower resin consumption than the flat‐base design group. However, the flat‐base designs exhibited superior trueness and less distortion after 1 year of storage. These findings indicate that despite the higher material usage, flat‐base designs provide better initial accuracy and maintain their dimensional stability over time for most groups.
Influence of gender on periodontal outcomes: A retrospective analysis of eight randomized clinical trials
Authors: Castro dos Santos Nidia , Westphal Miriam R. , Retamal‐Valdes Belen , Duarte Poliana Mendes , Figueiredo Luciene Cristina , Faveri Marcelo , Shibli Jamil , Soares Geisla , Miranda Tamires , Fermiano Daiane , Borges Ivan , Goncalves Cristiane , Tanaka Caio Junji , Teles Flavia , Goodson Max , Hasturk Hatice , Van Dyke Thomas , Ehmke Benjamin , Eickholz Peter , Nickles Katrin , Schlagenhauf Ulrich , Meyle Joerg , Kocher Thomas , Kim Ti‐Sun , Doyle Helio , Feres Magda 
Published: May 2024

AbstractAimsTo explore the influence of gender on periodontal treatment outcomes in a dataset of eight RCTs conducted in Brazil, United States, and Germany.MethodsClinical parameters were compared between men and women with stages III/IV grades B/C generalized periodontitis at baseline and 1‐year post‐therapy, including scaling and root planing with or without antibiotics.ResultsData from 1042 patients were analyzed. Men presented a tendency towards higher probing depth (p = .07, effect size = 0.11) and clinical attachment level (CAL) than women at baseline (p = .01, effect size = 0.16). Males also presented statistically significantly lower CAL gain at sites with CAL of 4–6 mm at 1‐year post‐therapy (p = .001, effect size = 0.20). Among patients with Grade B periodontitis who took antibiotics, a higher frequency of women achieved the endpoint for treatment (i.e., ≤4 sites PD ≥5 mm) at 1 year than men (p < .05, effect size = 0.12).ConclusionMen enrolled in RCTs showed a slightly inferior clinical response to periodontal therapy in a limited number of sub‐analyses when compared to women. These small differences did not appear to be clinically relevant. Although gender did not dictate the clinical response to periodontal treatment in this population, our findings suggest that future research should continue to explore this topic.
A causal trait model for explaining foliar water uptake capacity
Authors: Matos Ilaíne Silveira , Rifai Sami Walid , Gouveia Walquíria Felipe , Oliveras Imma , Mantuano Dulce , Rosado Bruno H. P. 
Published: May 2024

AbstractQuestionsPlants largely vary in their capacity for foliar water uptake (FWU), that is, the capacity to increase leaf water content by directly absorbing water from leaf‐wetting events. Climate change will reduce leaf wetting and increase drought events. Therefore, we need a better understanding of the underlying traits and mechanisms that facilitate FWU.LocationSeasonally dry tropical montane grasslands in Brazil (Campos de Altitude).MethodsWe measured FWU and leaf traits related to wettability, surface conductance, water potential and water storage on up to 55 plant species. By using Direct Acyclic Graph theory and Bayesian modelling, we tested how those leaf traits affect FWU.ResultsWe found that stomatal conductance largely explained interspecific variation in FWU, which was also favoured in species with hydrophilic leaves, high cuticular conductance, more negative leaf water potentials, low dry‐matter content, isohydric behaviour, and more elastic cell walls.ConclusionsDue to the existence of trade‐offs, not all species exhibit an optimal combination of traits that favours FWU. Instead, co‐occurring species have achieved a similar capacity for FWU through distinct trait combinations. Consequently, species engaged in FWU may exhibit differential vulnerabilities to climate change as they can cope with drought using other strategies beside FWU.