bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous analysis of six cardiovascular drugs by capillary electrophoresis coupled with electrochemical and electrochemiluminescence detection, using a chemometrical optimization approach
Authors: Li Xia , Zhu Derong , You Tianyan 
Published: July 2011

AbstractCE coupled with dual electrochemical (EC) and electrochemiluminescence (ECL) detection was optimized for simultaneous analysis of six cardiovascular drugs (alprenolol, propafenone, acebutolol, verapamil, atenolol and metoprolol) via central composite design. Following this study, three critical electrophoretic factors governing the CE separation were investigated: Tris‐H3PO4 buffer concentration, buffer pH value and separation voltage. A modified chromatographic response was adopted for evaluating CE separation quality. Optimum conditions were achieved using Tris‐H3PO4 buffer 35.6 mM (pH 2.3) separated at 13.9 kV, which was employed experimentally and led to the successful simultaneous separation of the above six drugs. The good agreement of the chromatographic response was observed between predicted data and actual experimental results using these optimized conditions (RSD=3.75%). The proposed method was validated for linearity, repeatability and sensitivity, and subsequently successfully applied to determine six basic drugs in urine samples.
Determination of nicotine and its metabolite cotinine in urine and cigarette samples by capillary electrophoresis coupled with electrochemiluminescence
Authors: Sun Jinying , Du Hongwei , You Tianyan 
Published: July 2011

AbstractIn this paper, CE coupled with electrochemiluminesence (ECL) detection using a 76‐μm Pt disk as working electrode was developed for nicotine (NIC) determination. The major metabolite of NIC is cotinine (COT), which has a similar tertiary amine structure to NIC. However, there is a carbonyl group attached in the structure of COT, which leads to the great decrease in ECL response. In order to improve the ECL response of COT, NaBH4 was used for carbonyl reduction. After reduction, NIC and COT were separated and detected by CE‐ECL. ECL response plotted with NIC concentration was linear between 5.0×10−7 and 5.0×10−5 mol/L (81–8100 μg/L), with LOD of 5.0×10−8 mol/L (8.1 μg/L). The developed CE‐ECL method was applied for NIC determination in urine and cigarette samples.
Dispersive liquid–liquid microextraction coupled with capillary electrophoresis for simultaneous determination of sulfonamides with the aid of experimental design
Authors: Wen Yingying , Li Jinhua , Zhang Weiwei , Chen Lingxin 
Published: July 2011

AbstractA novel method for the simultaneous determination of sulfonamides (SAs) in water samples has been developed by using dispersive liquid–liquid microextraction (DLLME) coupled with CE. Orthogonal and Box–Behnken designs were employed together to assist the optimization of DLLME parameters, including volumes of extraction and disperser solvents, ionic strength, extraction time, and centrifugation time and speed as variable factors. Under the optimum extraction and detection conditions, successful separation of the five SAs was achieved within 5 min, and excellent analytical performances were attained, such as good linear relationships (R>0.980) between peak area and concentration for each SA from 0.5 to 50 μg/mL, low limits of detection for the five SAs between 0.020 and 0.570 μg/mL and the intra‐day precisions of migration time below 0.80%. The method recoveries obtained at fortified 10 μg/mL for three water samples ranged from 53.6 to 94.0% with precisions of 1.23–5.60%. The proposed method proved highly sensitive and selective, rapid, convenient and cost‐effective, showing great potential for the simultaneous determination of SAs in water samples.
Thermal stabilization of tissues and the preservation of protein phosphorylation states for two‐dimensional gel electrophoresis
Authors: Smejkal Gary B. , Rivas‐Morello Chiara , Chang Jae‐Hyung Robert , Freeman Emily , Trachtenberg Alexander J. , Lazarev Alexander , Ivanov Alexander R. , Kuo Winston P. 
Published: July 2011

Abstract2‐DE is typically capable of discriminating proteins differing by a single phosphorylation or dephosphorylation event. However, a reliable representation of protein phosphorylation states as they occur in vivo requires that both phosphatases and kinases are rapidly and completely inactivated. Thermal stabilization of mouse cerebral cortex homogenates effectively inactivated these enzymes, as evidenced by comparison with unstabilized tissues where abscissal pI shifts were a common feature in 2‐D gels. Of the 588 matched proteins separated on 2‐D gels comparing stabilized and unstabilized tissues, 53 proteins exhibited greater than twofold differences in spot volume (ANOVA, p<0.05). Phosphoprotein‐specific staining was corroborated by the identification of 16 phosphoproteins by nano‐LC MS/MS and phosphotyrosine kinase activity assay.
Historical floods and dendrochronological dating of a wooden deck in the Old Mint of Segovia, Spain
Authors: Génova Mar , Ballesteros‐Cánovas Juan A. , Díez‐Herrero Andres , Martínez‐Callejo Begoña 
Published: July 2011

AbstractDuring recent archaeological work carried out in the 16th‐century Segovia Mint, Spain, a wooden deck of Pinus sylvestris was revealed covering the hydraulic canals. Dendrochronological analyses allowed us to estimate the dates that trees were felled in order to effect repairs to canals damaged after extraordinary flood events. There are at least three age clusters: (1) 1677–1678; (2) 1690–1698–1700; and (3) 1713–1720–1721–1742. By comparing dendrochronological data with both documentary records of repair work and the inventory of historical floods, the dates of the main floods affecting the building between 1583 and 1771 can be identified. Our work is innovative in that it uses tree‐ring dating in historical buildings to reconstruct past flood events, which are then linked to documentary records. It has three main applications: validating documented flood dates, estimating their relative magnitude, and locating new non‐documented flood events or previously unknown historical repair work at this UNESCO World Heritage Site. © 2011 Wiley Periodicals, Inc.
Employees' attitudes toward organizational change: A literature review
Authors: Choi Myungweon 
Published: July 2011

AbstractOrganizations are increasingly required to improve their ability to enhance employees' support or acceptance for change initiatives. In studies that have examined the conditions in which employees support organizational change, researchers have focused on various attitudinal constructs that represent employees' attitudes toward organizational change. The constructs, which frequently serve as key variables in these studies, include readiness for change, commitment to change, openness to change, and cynicism about organizational change. These constructs have distinct meanings and emphases and therefore they can provide us with different information regarding employees' evaluation of and concerns about particular change initiatives. In this literature review, the author discusses how the constructs are defined in the organizational change literature and synthesizes the antecedents of each construct. Based on the discussion, it is proposed that the constructs are susceptible to situational variables, and may change over time as individuals' experiences change; therefore, they are better conceptualized as states than as personality traits. ©2011 Wiley Periodicals, Inc.
Linking capacities of high‐quality relationships to team learning and performance in service organizations
Authors: Brueller Daphna , Carmeli Abraham 
Published: July 2011

AbstractThis study examines the impact of intrateam and external high‐quality relationships (HQRs) on learning processes and performance. Data collected from 178 teams in the service sector indicate that (a) intrateam HQRs (i.e., between team members and between team members and their manager) are related to psychological safety, which in turn facilitates learning processes; (b) external HQRs are associated directly with team learning; and (c) team learning is positively associated with enhanced team performance. The findings highlight the importance of both internal and external HQRs to facilitate learning and enhance performance in service organizations. © 2011 Wiley Periodicals, Inc.
Socialization of people with disabilities in the workplace
Authors: Kulkarni Mukta , Lengnick‐Hall Mark L. 
Published: July 2011

AbstractSocialization has crucial outcomes for both the employee and the employer. Through an exploratory qualitative study conducted in India, we examined how people with disabilities (PWD) viewed various aspects of their socialization process. Specifically, we looked at the role of coworkers, supervisors, organizational practices, and employee proactive behaviors in influencing organizational integration. We found that integration was most influenced by coworkers and supervisors. Organizational practices and employee proactive behaviors were less important. Respondent gender and tenure also influenced certain findings. Specifically, PWD with less tenure sought and accepted more help from coworkers and supervisors. Further, more men with disabilities than women with disabilities indicated that they were proactive in terms of obtaining training to make themselves employable, and more men with disabilities indicated that having coworkers with a disability helped them during socialization. We discuss both theoretical and practical implications as well as future research directions based on our findings. © 2011 Wiley Periodicals, Inc.
Organizational achievement values, high‐involvement work practices, and business unit performance
Authors: O'Neill Olivia A. , Feldman Daniel C. , Vandenberg Robert J. , DeJoy David M. , Wilson Mark G. 
Published: July 2011

AbstractThis study examines the relationships among organizational achievement values, high‐involvement work practices (HIWP), and business unit performance. Specifically, we examine whether HIWP mediate or moderate the effect of achievement values on retail store performance. Using data collected at three points in time from more than 1,000 employees, we found support for the mediation model. The article concludes with directions for future research and implications for management practice. © 2011 Wiley Periodicals, Inc.
Leadership change and shareholder value: How markets react to the appointments of women
Authors: Cook Alison , Glass Christy 
Published: July 2011

AbstractThis study examines whether the appointment of women into senior leadership positions has a more positive effect on share price than the appointment of men into equivalent positions. Our dependent variable is the degree of change in share price following the announcement of men and women into senior leadership positions. Although market reactions to corporate events represent a complex process, we argue that changes in stock price represent a barometer for how investors assess the decision's potential effect on a corporation's short‐ and long‐term economic viability. We find a significant spike in stock price following the announcement of women into top leadership positions. The size and direction of change in stock price, however, is moderated by the gender composition of the industry. © 2011 Wiley Periodicals, Inc.
Effect of bed surface roughness on longitudinal dispersion in artificial open channels
Authors: Schulz Marcus , Priegnitz Jan , Klasmeier Jörg , Heller Stefan , Meinecke Stefan , Feibicke Michael 
Published: July 2011

AbstractIn several empirical and modelling studies on river hydraulics, dispersion was negatively correlated to surface roughness. In this study, it was aimed to investigate the influence of surface roughness on longitudinal dispersion under controlled conditions. In artificial flow channels with a length of 104 m, tracer experiments with variations in channel bed material were performed. By use of measured tracer breakthrough curves, average flow velocity, mean longitudinal dispersion, and mean longitudinal dispersivity were calculated. Longitudinal dispersion coefficients ranged from 0·018 m2 s−1 in channels with smooth bed surface up to 0·209 m2 s−1 in channels with coarse gravel as bed material. Longitudinal dispersion was linearly related to mean flow velocity. Accordingly, longitudinal dispersivities ranged between 0·152 ± 0·017 m in channels with smooth bed surface and 0·584 ± 0·015 m in identical channels with a coarse gravel substrate. Grain size and surface roughness of the channel bed were found to correlate positively to longitudinal dispersion. This finding contradicts several existing relations between surface roughness and dispersion. Future studies should include further variation in surface roughness to derive a better‐founded empirical equation forecasting longitudinal dispersion from surface roughness. Copyright © 2011 John Wiley & Sons, Ltd.
Influence diagrams as decision-making tools for pesticide risk management
Authors: Carriger John F , Newman Michael C 
Published: July 2011

Abstract
The pesticide policy arena is filled with discussion of probabilistic approaches to assess ecological risk, however, similar discussions about implementing formal probabilistic methods in pesticide risk decision making are less common. An influence diagram approach is proposed for ecological risk-based decisions about pesticide usage. Aside from technical data, pesticide risk management relies on diverse sources, such as stakeholder opinions, to make decisions about what, how, where, and when to spray. Bayesian influence diagrams allow multiple lines of evidence, including process related information from existing data and expert judgment, in 1 inclusive decision model. In ecological risk assessments, data informally incorporated for pesticide usage decisions, such as field and laboratory effect studies along with chemical monitoring and modeling data, can be formally incorporated and expressed in linked causal diagrams. A case study is presented from the perspective of an environmental manager wishing to efficiently control pests while minimizing risk to local aquatic receptors. Exposure modeling results and toxicity studies were incorporated, and an ecological risk assessment was carried out but combined with hypothetical information on spraying efficacy and valuation of outcomes that would be necessary for making risk management decisions. The variables and their links in the influence diagram are ones that are important to a manager and can be manipulated to optimally control pests while protecting nontarget resources. Integr Environ Assess Manag 2012; 8: 339–350. © 2011 SETAC
Environmental engineering of navigation infrastructure: A survey of existing practices, challenges, and potential opportunities
Authors: Fredette Thomas J , Foran Christy M , Brasfield Sandra M , Suedel Burton C 
Published: July 2011

Abstract
Navigation infrastructure such as channels, jetties, river training structures, and lock-and-dam facilities are primary components of a safe and efficient water transportation system. Planning for such infrastructure has until recently involved efforts to minimize impacts on the environment through a standardized environmental assessment process. More recently, consistent with environmental sustainability concepts, planners have begun to consider how such projects can also be constructed with environmental enhancements. This study examined the existing institutional conditions within the US Army Corps of Engineers and cooperating federal agencies relative to incorporating environmental enhancements into navigation infrastructure projects. The study sought to (1) investigate institutional attitudes towards the environmental enhancement of navigation infrastructure (EENI) concept, (2) identify potential impediments to implementation and solutions to such impediments, (3) identify existing navigation projects designed with the express intent of enhancing environmental benefit in addition to the primary project purpose, (4) identify innovative ideas for increasing environmental benefits for navigation projects, (5) identify needs for additional technical information or research, and (6) identify laws, regulations, and policies that both support and hinder such design features. The principal investigation tool was an Internet-based survey with 53 questions. The survey captured a wide range of perspectives on the EENI concept including ideas, concerns, research needs, and relevant laws and policies. Study recommendations included further promotion of the concept of EENI to planners and designers, documentation of existing projects, initiation of pilot studies on some of the innovative ideas provided through the survey, and development of national goals and interagency agreements to facilitate implementation. Integr Environ Assess Manag 2012;8:1–1. © 2011 SETAC
Myosin heavy chain is not selectively decreased in murine cancer cachexia
Authors: Cosper Pippa F. , Leinwand Leslie A. 
Published: July 2011

AbstractCachexia is a severe muscle‐wasting syndrome associated with several chronic diseases such as cancer and AIDS. Muscle mass loss significantly decreases prognosis and survival. The mechanisms of muscle atrophy and the specific proteins targeted for degradation have been intensely studied and are potential therapeutic targets. Published reports that myosin heavy chain (MyHC), the most abundant protein by mass in skeletal muscle, is selectively targeted for degradation in cancer cachexia remain controversial. Here we show that the results of previous studies showing a selective decrease in MyHC are likely an artifact resulting from muscle lysis methods which do not solubilize myosin out of myofibrils. We show that MyHC decreases in parallel with other myofibrillar proteins in cachectic skeletal muscle, which has mechanistic and therapeutic implications. These findings should lead to mechanistic insight into the stoichiometry of sarcomeric disassembly and degradation during cancer cachexia.
Inactivation of the putative suppressor gene DOK1 by promoter hypermethylation in primary human cancers
Authors: Saulnier Amandine , Vaissière Thomas , Yue Jiping , Siouda Maha , Malfroy Marine , Accardi Rosita , Creveaux Marion , Sebastian Sinto , Shahzad Naveed , Gheit Tarik , Hussain Ishraq , Torrente Mariela , Maffini Fausto Antonio , Calabrese Luca , Chiesa Fausto , Cuenin Cyrille , Shukla Ruchi , Fathallah Ikbal , Matos Elena , Daudt Alexander , Koifman Sergio , Wünsch‐Filho Victor , Menezes Ana M.B. , Curado Maria‐Paula , Zaridze David , Boffetta Paolo , Brennan Paul , Tommasino Massimo , Herceg Zdenko , Sylla Bakary S. 
Published: July 2011

AbstractThe DOK1 gene is a putative tumour suppressor gene located on the human chromosome 2p13 which is frequently rearranged in leukaemia and other human tumours. We previously reported that the DOK1 gene can be mutated and its expression down‐regulated in human malignancies. However, the mechanism underlying DOK1 silencing remains largely unknown. We show here that unscheduled silencing of DOK1 expression through aberrant hypermethylation is a frequent event in a variety of human malignancies. DOK1 was found to be silenced in nine head and neck cancer (HNC) cell lines studied and DOK1 CpG hypermethylation correlated with loss of gene expression in these cells. DOK1 expression could be restored via demethylating treatment using 5‐aza‐2′deoxycytidine. In addition, transduction of cancer cell lines with DOK1 impaired their proliferation, consistent with the critical role of epigenetic silencing of DOK1 in the development and maintenance of malignant cells. We further observed that DOK1 hypermethylation occurs frequently in a variety of primary human neoplasm including solid tumours (93% in HNC, 81% in lung cancer) and haematopoietic malignancy (64% in Burkitt's lymphoma). Control blood samples and exfoliated mouth epithelial cells from healthy individuals showed a low level of DOK1 methylation, suggesting that DOK1 hypermethylation is a tumour specific event. Finally, an inverse correlation was observed between the level of DOK1 gene methylation and its expression in tumour and adjacent non tumour tissues. Thus, hypermethylation of DOK1 is a potentially critical event in human carcinogenesis, and may be a potential cancer biomarker and an attractive target for epigenetic‐based therapy.
Identification of a novel recurrent 1q42.2‐1qter deletion in high risk MYCN single copy 11q deleted neuroblastomas
Authors: Fieuw Annelies , Kumps Candy , Schramm Alexander , Pattyn Filip , Menten Björn , Antonacci Francesca , Sudmant Peter , Schulte Johannes H. , Van Roy N , Vergult Sarah , Buckley Patrick G. , De Paepe A , Noguera Rosa , Versteeg Rogier , Stallings Raymond , Eggert Angelika , Vandesompele Jo , De Preter K , Speleman Frank 
Published: July 2011

AbstractNeuroblastoma is an aggressive embryonal tumor that accounts for ∼15% of childhood cancer deaths. Hitherto, despite the availability of comprehensive genomic data on DNA copy number changes in neuroblastoma, relatively little is known about the genes driving neuroblastoma tumorigenesis. In this study, high resolution array comparative genome hybridization (CGH) was performed on 188 primary neuroblastoma tumors and 33 neuroblastoma cell lines to search for previously undetected recurrent DNA copy number gains and losses. A new recurrent distal chromosome 1q deletion (del(1)(q42.2qter)) was detected in seven cases. Further analysis of available array CGH datasets revealed 13 additional similar distal 1q deletions. The majority of all detected 1q deletions was found in high risk 11q deleted tumors without MYCN amplification (Fisher exact test p = 5.61 × 10−5). Using ultra‐high resolution (∼115 bp resolution) custom arrays covering the breakpoints on 1q for 11 samples, clustering of nine breakpoints was observed within a 12.5‐kb region, of which eight were found in a 7‐kb copy number variable region, whereas the remaining two breakpoints were colocated 1.4‐Mb proximal. The commonly deleted region contains one miRNA (hsa‐mir‐1537), four transcribed ultra conserved region elements (uc.43‐uc.46) and 130 protein coding genes including at least two bona fide tumor suppressor genes, EGLN1 (or PHD2) and FH. This finding further contributes to the delineation of the genomic profile of aggressive neuroblastoma, offers perspectives for the identification of genes contributing to the disease phenotype and may be relevant in the light of assessment of response to new molecular treatments.