bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying transient protein–protein interactions in EphB2 signaling by blue native PAGE and mass spectrometry
Authors: Darie Costel C. , Deinhardt Katrin , Zhang Guoan , Cardasis Helene S. , Chao Moses V. , Neubert Thomas A. 
Published: September 2011

AbstractReceptor tyrosine kinases (RTKs) are proteins that upon ligand stimulation undergo dimerization and autophosphorylation. Eph receptors (EphRs) are RTKs that are found in different cell types, from both tissues that are developing and from mature tissues, and play important roles in the development of the central nervous system and peripheral nervous system. EphRs also play roles in synapse formation, neural crest formation, angiogenesis and in remodeling the vascular system. Interaction of EphRs with their ephrin ligands lead to activation of signal transduction pathways and formation of many transient protein–protein interactions that ultimately leads to cytoskeletal remodeling. However, the sequence of events at the molecular level is not well understood. We used blue native PAGE and MS to analyze the transient protein–protein interactions that resulted from the stimulation of EphB2 receptors by their ephrinB1‐Fc ligands. We analyzed the phosphotyrosine‐containing protein complexes immunoprecipitated from the cell lysates of both unstimulated (−) and ephrinB1‐Fc‐stimulated (+) NG108 cells. Our experiments allowed us to identify many signaling proteins, either known to be part of EphB2 signaling or new for this pathway, which are involved in transient protein–protein interactions upon ephrinB1‐Fc stimulation. These data led us to investigate the roles of proteins such as FAK, WAVEs and Nischarin in EphB2 signaling.
Effects of hydration and hydrophilicity on Na‐Clay‐supported aqueous‐phase catalysis for atom transfer radical polymerization
Authors: Aggarwal Ravi , Baskaran Durairaj 
Published: September 2011

AbstractHydrated sodium montmorillonite (Na‐clay) has been used as a catalyst support for the heterogeneous atom transfer radical polymerization of benzyl methacrylate in the presence of various concentrations of water, reducing agent, and CuBr2 in anisole at ambient temperature. The polymerization was promoted via reduction of CuII to CuI through the addition of sodium ascorbate (NaAsc) as a reducing agent in aqueous solution. The polymerizaton proceeded in a controlled manner and produced poly(benzyl methacylate) with moderately narrow molecular weight distribution (MWD) when performed under optimum conditions of hydration (10 wt % ≤ H2O/Na‐clay ≤ 21 wt %) and reducing agent (0.15 ≤ [NaAsc]/[I] ≤ 0.23). The polymerization was uncontrolled if hydration and NaAsc exceed above their optimum range of concentrations. Apparent rate of the polymerization (kapp) increased in the presence of decane–anisole (1/3, v/v) mixture solvent. Selective adsorption of decane at the interfaces of the hydrated clay was attributed for the rate enhancement due to increased polymer and hydrophobic interface interaction. The polymerization progressed in a controlled manner as confirmed by the first‐order time‐conversion plot, linear increase in molecular weights, and moderately narrow MWDs over conversion. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Development of high‐performance networked polymers consisting of isocyanurate structures based on selective cyclotrimerization of isocyanates
Authors: Moritsugu Masaki , Sudo Atsushi , Endo Takeshi 
Published: September 2011

AbstractSelective and quantitative cyclotrimerization of p‐tolylisocyanate proceeded by using sodium p‐toluenesulfinate as a catalyst and 1,3‐dimethylimidazolidinone as a solvent. Exploitation of this system to the cyclotrimerization of methylene diphenyl 4,4′‐diisocyanate (MDI) permitted formation of the corresponding networked polymer, which was selectively consisted of isocyanurate moiety and thus exhibited excellent thermal stability. Utilization of phenyl isocyanate (PhNCO) as a comonomer with varying feed ratio [MDI]0/[PhNCO]0 allowed successful control of flexibility of the networked polymers, while retaining its high thermal stability. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Controlled polymerization of epoxides: Metal‐free ring‐opening polymerization of glycidyl phenyl ether initiated by tetra‐n‐butylammonium fluoride in the presence of protic compounds
Authors: Morinaga Hisatoyo , Ujihara Yusuke , Yuto Naho , Nagai Daisuke , Endo Takeshi 
Published: September 2011

AbstractMetal‐free controlled ring‐opening polymerization of glycidyl phenyl ether (GPE) was achieved using tetra‐n‐butylammonium fluoride (Bu4NF) as an initiator in the presence of water and ethanol as chain transfer agents (CTAs). Number‐averaged molecular weight of poly(GPE) increased with an increase of [GPE]0/([Bu4NF]0 + [CTA]0) values, showing relatively narrow molecular weight distributions. NMR spectroscopic analysis exhibited a formation of ethoxy groups as well as FCH2 at the initiating polymer chain‐end when ethanol was used as the CTA in the polymerization. These results indicate that Bu4NF acts as a catalyst as well as the initiator for this polymerization system. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Identifying how and for whom cognitive‐behavioral stress management improves emotional well‐being among recent prostate cancer survivors
Authors: Traeger Lara , Penedo Frank J. , Benedict Catherine , Dahn Jason R. , Lechner Suzanne C. , Schneiderman Neil , Antoni Michael H. 
Published: September 2011

AbstractObjectiveThe outcomes of a 10‐week cognitive‐behavioral stress management (CBSM) group intervention were evaluated in prostate cancer survivors. A model was tested in which CBSM‐related improvements in emotional well‐being were attained through changes in men's perceptions of their condition, as conceptualized by information processing explanations of self‐regulation theory. The model also tested whether life stress and treatment‐related side effects moderated intervention effects.MethodsMen treated for localized prostate cancer (n = 257) within the past 18 months were randomized to CBSM or a half‐day psycho‐educational seminar. At pre‐intervention and 12‐week follow‐up, emotional well‐being, illness perceptions, life stress, and sexual and urinary function were assessed using validated questionnaires.ResultsAfter controlling for covariates, CBSM participants showed greater improvements in emotional well‐being relative to control participants (β = 0.13, p < 0.05). For men reporting higher stress upon study entry, CBSM‐related improvements were partially explained by changes in some, but not all, illness perceptions. Sexual and urinary dysfunction did not influence CBSM‐related gains.ConclusionsProstate cancer perceptions may be an important target for enhancing emotional well‐being, particularly for men experiencing general life stress. However, interventions that explicitly target mental representations of cancer may be needed to modify perceptions of the disease. Copyright © 2011 John Wiley & Sons, Ltd.
Tryptophan side chain conformers monitored by NMR and time‐resolved fluorescence spectroscopies
Authors: Julien Olivier , Wang Guoli , Jonckheer Abel , Engelborghs Yves , Sykes Brian D. 
Published: September 2011

AbstractWe have inserted a tryptophan (F77W) in the core of the regulatory domain of cardiac troponin C (cNTnC), and previously determined the structure of this mutant with and without the cosolvent trifluoroethanol (TFE). Interestingly, the orientations of the indole side chain of the Trp are in opposite directions in the two structures (Julien et al., Protein Sci 2009; 18:1165–1174). Fluorescence decay experiments for single Trp‐containing proteins often show several lifetimes, which have been interpreted as reflecting conformational heterogeneity of the Trp side chain resulting from different rotamers. To test this interpretation, we monitored the effect of TFE on wild type, F77W and F77W‐V82A calcium‐saturated cNTnC using 2D 13C‐HSQC NMR and time‐correlated single photon counting fluorescence spectroscopies. The time dependence of the Trp fluorescence decay was fit with three lifetimes. Addition of TFE caused a gradual, but limited decrease of the lifetimes due to dynamic quenching. For F77W cNTnC, the amplitude fractions of the lifetimes also changed upon addition of TFE—the long lifetime increased from 13 to 29%, while the middle lifetime decreased from 63 to 50% and the short lifetime remained relatively unchanged. For F77W‐V82A cNTnC, comparable NMR changes are observed, confirming the switch in rotamer conformation, but only much smaller changes in fluorescence decay parameters were detected. These data indicate that the balance between the rotamer states can be changed without changing the lifetime amplitude fractions appreciably, and suggest that the environment(s) of the indole ring, responsible for the different lifetimes, can result from factors other than the intrinsic rotamer state of the tryptophan. Proteins 2012; © 2011 Wiley Periodicals, Inc.
Effects on Raman spectra of functionalisation of single walled carbon nanotubes by nitric acid
Authors: Mases M. , Noël M. , Mercier G. , Dossot M. , Vigolo B. , Mamane V. , Fort Y. , Soldatov A. V. , McRae E. 
Published: September 2011

AbstractIn the ultimate aim of grafting a fluorescent group on carbon nanotubes (CNTs) using COOH functions as anchoring groups, it was realised that optimisation of the carboxylation step of the CNTs was essential in the overall process. To reach this goal, three different treatment times with refluxed nitric acid have been tested: 2, 5 and 10 h. Electron microscopy has allowed evaluating the microstructure changes and the chemical composition on a local level. Raman spectroscopy has revealed a number of interesting evolutions especially in the D and G bands spectral region. It seems that residual nitric acid molecules may partially transfer charge to CNTs, giving rise to a doping effect, as is well known in graphite intercalation compounds.
Electronic state of polyaniline deposited on carbon nanotube or ordered mesoporous carbon templates
Authors: Bulusheva L. G. , Fedorovskaya E. O. , Okotrub A. V. , Maximovskiy E. A. , Vyalikh D. V. , Chen Xiaohong , Song Huaihe 
Published: September 2011

AbstractPolyaniline (PANI) has been deposited on a carbon template by chemical oxidation of aniline chloride in acidic aqueous medium using sodium persulfate as oxidant. Two kinds of templates were used: array of aligned multiwall carbon nanotubes (CNTs) grown on silicon substrate and ordered mesoporous carbon (OMC) material. Electronic structure of composites was examined using near‐edge X‐ray absorption spectroscopy and X‐ray photoelectron spectroscopy. Analysis of the spectra showed that PANI developed on the CNT surface is mainly protonated while it contains a marked amount of imine (N) nitrogen when deposited into the porous of OMC. Testing of electrochemical cell indicated enhancement of performance of composite electrodes as compared with unsupported PANI. magnified image
XPS N 1s spectra of composites of PANI with OMC material (PANI/OMC) and aligned multiwall CNTs (PANI/CNT).
A method for fast encoder‐free mapping in unstructured environments
Authors: Milstein Adam , McGill Matthew , Wiley Timothy , Salleh Rudino , Sammut Claude 
Published: September 2011

AbstractThe primary purpose of rescue robots is to locate victims within a disaster environment, either to report their position or to deliver aid. Although there exist several solutions for mapping and localizing a robot in an unknown environment, they often require an estimate of the robot's motion. In a disaster site, a robot might not have effective motion encoders, either because of the discontinuous nature of the terrain or because the robot's locomotion devices do not remain in constant contact with the ground. We have developed an algorithm for tracking the position of a robot in a disaster environment and generating a map that functions in real time. Our solution is based on a range finder device and is robust to temporary errors in the range scan. By aligning each scan to an occupancy grid of prior scan data, we can find the robot's position more accurately than current techniques that align only to the previous scan. The accurate position tracking allows us to more efficiently implement a solution to the mapping problem. In addition, our solution can track the position of the robot and generate a map based on three‐dimensional scan data, instead of requiring that the range sensor be fixed in a level plane. © 2011 Wiley Periodicals, Inc.
Perceived Neighborhood Social Resources as Determinants of Prosocial Behavior in Early Adolescence
Authors: Lenzi Michela , Vieno Alessio , Perkins Douglas D. , Pastore Massimiliano , Santinello Massimo , Mazzardis Sonia 
Published: September 2011

AbstractThe present study aims to develop an integrative model that links neighborhood behavioral opportunities and social resources (neighborhood cohesion, neighborhood friendship and neighborhood attachment) to prosocial (sharing, helping, empathic) behavior in early adolescence, taking into account the potential mediating role of perceived support of friends. Path analysis was used to test the proposed theoretical model in a sample of 1,145 Italian early adolescents (6th through 8th graders). More perceived opportunities and social resources in the neighborhood are related to higher levels of adolescent prosocial behavior, and this relationship is partially mediated by perceived social support from friends. The results offer promising implications for future research and intervention programs that aim to modify social systems to improve child and adolescent social competencies.
Hippocampal atrophy as a surrogate of neuronal involvement in Fabry disease
Authors: Fellgiebel Andreas , Wolf Dominik O. , Kolodny Edwin , Müller Matthias J. 
Published: September 2011

AbstractCerebral micro‐ and macro‐vasculopathy have been described in Fabry disease (FD). Neuronal globotriaosylceramide accumulation in selective cortical and brain stem areas including the hippocampus has been reported by autopsy studies in FD, but clinical surrogates as well as the clinical relevance of these findings have not been investigated so far. We measured the hippocampus volumes in a group of clinically affected patients with FD and correlated the findings with the cognitive performance of the patients. Hippocampal volumes were determined manually on T1‐weighted MR‐images of 25 FD patients (age 36.5 ± 11.0 years) and 20 age‐matched controls. Additionally, individual white matter (WM) and gray matter (GM) volumes were measured using brain segmentation analyses. After controlling for age, white matter lesion (WML) volume, and WM/GM‐volumes hippocampal volumes were significantly decreased in FD. These findings were substantially more pronounced in a subgroup of men with FD. WM and WM/GM volumes, and memory function did not significantly differ between patients and controls. In patients with FD hippocampal volumes were neither significantly correlated to WML volume nor to WM or WM/GM volumes. Hippocampus atrophy was not driven by the WML or other brain tissue atrophy and seems to correlate with the neuronal involvement in FD. In this young to middle‐aged Fabry cohort the hippocampus degeneration was functionally compensated without memory impairment. Longitudinal studies are needed to determine whether this degenerative component in FD will progress and, in concert with the individual WML‐load, predict subsequent cognitive decline.
A programmed schedule of oocyte retrieval using mild ovarian stimulation (clomiphene citrate and recombinant follicle‐stimulating hormone)
Authors: Nakagawa Koji , Nishi Yayoi , Sugiyama Rie , Jyuen Hiroyasu , Takahashi Chie , Ojiro Yuko , Kuribayashi Yasushi , Sugiyama Rikikazu 
Published: September 2011

AbstractPurposeThe purpose was to establish a mild ovarian stimulation protocol that would help assisted reproductive technology (ART) units to avoid scheduling on weekends.MethodsThis protocol directed patients to take 50 mg/day of clomiphene citrate between days 3 and 7 of the menstrual cycle: 225 IU of recombinant follicle‐stimulating hormone (rec‐FSH) were administered on days 3, 5 and 7; human chorionic gonadotropin (hCG) was administered on day 9; and, oocyte pick‐up (OPU) was planned for day 11. From October 2008 through October 2009, 514 women underwent ART treatment with mild stimulation at the Sugiyama Clinic, and we evaluated whether OPU was accomplished on the planned day.ResultsOf all the treatment cycles, 419 (81.5%) underwent OPU on day 11 (scheduled group). Additional rec‐FSH administration was needed in 83 cycles, in which case OPU was performed on day 12 or later. In 12 cycles, OPU was canceled. The unscheduled group (n = 95) consisted of delayed OPU cycles and canceled cycles. Of all treatment cycles, 332 cycles in the scheduled group and 68 cycles in the unscheduled group underwent embryo transfer, with 81 and 16, respectively, resulting in pregnancies.ConclusionsUsing this protocol, OPU was performed on the scheduled day in about 80% of the cycles. Most weekend scheduling of OPU can be avoided using this mild stimulation.
Streaming-potential phenomena in the thin-Debye-layer limit. Part 1. General theory
Authors: Yariv Ehud , Schnitzer Ory , Frankel Itzchak 
Published: September 2011

AbstractElectrokinetic streaming-potential phenomena are driven by imposed relative motion between liquid electrolytes and charged solids. Owing to non-uniform convective ‘surface’ current within the Debye layer Ohmic currents from the electro-neutral bulk are required to ensure charge conservation thereby inducing a bulk electric field. This, in turn, results in electro-viscous drag enhancement. The appropriate modelling of these phenomena in the limit of thin Debye layers $\delta \ensuremath{\rightarrow} 0$ ($\delta $ denoting the dimensionless Debye thickness) has been a matter of ongoing controversy apparently settled by Cox’s seminal analysis (J. Fluid Mech., vol. 338, 1997, p. 1). This analysis predicts electro-viscous forces that scale as ${\delta }^{4} $ resulting from the perturbation of the original Stokes flow with the Maxwell-stress contribution only appearing at higher orders. Using scaling analysis we clarify the distinction between the normalizations pertinent to field- and motion-driven electrokinetic phenomena, respectively. In the latter class we demonstrate that the product of the Hartmann & Péclet numbers is $O({\delta }^{\ensuremath{-} 2} )$ contrary to Cox (1997) where both parameters are assumed $O(1)$. We focus on the case where motion-induced fields are comparable to the thermal scale and accordingly present a singular-perturbation analysis for the limit where the Hartmann number is $O(1)$ and the Péclet number is $O({\delta }^{\ensuremath{-} 2} )$. Electric-current matching between the Debye layer and the electro-neutral bulk provides an inhomogeneous Neumann condition governing the electric field in the latter. This field, in turn, results in a velocity perturbation generated by a Smoluchowski-type slip condition. Owing to the dominant convection, the present analysis yields an asymptotic structure considerably simpler than that of Cox (1997): the electro-viscous effect now already appears at $O({\delta }^{2} )$ and is contributed by both Maxwell and viscous stresses. The present paradigm is illustrated for the prototypic problem of a sphere sedimenting in an unbounded fluid domain with the resulting drag correction differing from that calculated by Cox (1997). Independently of current matching, salt-flux matching between the Debye layer and the bulk domain needs also to be satisfied. This subtle point has apparently gone unnoticed in the literature, perhaps because it is trivially satisfied in field-driven problems. In the present limit this requirement seems incompatible with the uniform salt distribution in the convection-dominated bulk domain. This paradox is resolved by identifying the dual singularity associated with the limit $\delta \ensuremath{\rightarrow} 0$ in motion-driven problems resulting in a diffusive layer of $O({\delta }^{2/ 3} )$ thickness beyond the familiar $O(\delta )$-wide Debye layer.
Retirement Lost?
Authors: McDonald Lynn , Donahue Peter 
Published: September 2011

RÉSUMÉCet article soulève la question de savoir si la retraite, comme nous la connaissons et comprenons actuellement au Canada, est perdue. Grâce à un examen sélectif, nous examinons la recherche sur la retraite selon la durée de la retraite, de nouveaux types de retraite, les évolutions théoriques possibles, les moments de transition vers la retraite, et la vie des retraités, y inclus la qualité ou le manque de pensions. En conséquence, nous proposons que la retraite est soumise a des modifications sur la base de plusieurs tendances qui ont commencé avant le ralentissement économique de 2008. Les données semblent indiquer l’émergence d’un type différent de la retraite, dans la mesure où la vision collective canadienne de la retraite n’est pas perdue, malgré la crise économique sur les marchés mondiaux.
Enrichment culture can bias the isolation ofCampylobactersubtypes
Authors: WILLIAMS L. K. , SAIT L. C. , COGAN T. A. , JØRGENSEN F. , GROGONO-THOMAS R. , HUMPHREY T. J. 
Published: September 2011

SUMMARYEnrichment culture is often used to isolateCampylobacter. This study compared isolation ofCampylobacterspp. from 119 broiler chicken environments from two farms, using Preston and modified Exeter (mExeter) and modified Bolton (mBolton) enrichments. mExeter was significantly more effective in isolatingCampylobacterspp. from the environmental samples compared to Preston (P<0·001) and mBolton (P<0·04) broths but there was no significant difference between the latter two methods (P>0·05). Enrichment broth type did not affect isolation from chicken faecal or soil and litter samples.C. jejuniwas isolated from significantly more environmental samples using mExeter broth compared to Preston (P<0·01) and mBolton (P<0·003) broths; there was no difference between the latter two methods or between all methods for detection ofC. coli(P>0·05). OnlyC. coliwas isolated from the soil and litter samples and although bothC. jejuniandC. coliwere recovered from the faecal samples there was no effect of using different enrichment broths. The majority of samples where the same species had been isolated yielded the same or closely related genotypes as defined by pulsed-field gel electrophoresis. Isolates recovered using Preston and mBolton broths were less genetically diverse than those from mExeter broth. We conclude that the enrichment method used affects both the number and species ofCampylobacterisolated from naturally contaminated samples.