bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct comparison of Hoxb8-driven reporter distribution in the brains of four transgenic mouse lines: towards a spinofugal projection atlas
Authors: Barraclough Bridget N. , Stubbs W. Terrence , Bohic Manon , Upadhyay Aman , Abraira Victoria E. , Ramer Matt S. 
Published: May 2024

IntroductionHox genes govern rostro-caudal identity along the developing spinal cord, which has a well-defined division of function between dorsal (sensory) and ventral (motor) halves. Here we exploit developmental Hoxb8 expression, normally restricted to the dorsal cord below the obex, to genetically label spinal cord-to-brain (“spinofugal”) axons.MethodsWe crossed two targeted (knock-in) and two non-targeted recombinase-expressing lines (Hoxb8-IRES-Cre and Hoxb8-T2AFlpO; Hoxb8-Cre and Hoxb8-FlpO, respectively) with appropriate tdtomato-expressing reporter strains. Serial sectioning, confocal and superresolution microscopy, as well as light-sheet imaging was used to reveal robust labeling of ascending axons and their terminals in expected and unexpected regions.ResultsThis strategy provides unprecedented anatomical detail of ascending spinal tracts anterior to the brainstem, and reveals a previously undescribed decussating tract in the ventral hypothalamus (the spinofugal hypothalamic decussating tract, or shxt). The absence of Hoxb8-suppressing elements led to multiple instances of ectopic reporter expression in Hoxb8-Cre mice (retinal ganglion and vomeronasal axons, anterior thalamic nuclei and their projections to the anterior cingulate and retrosplenial cortices and subiculum, and a population of astrocytes at the cephalic flexure) and Hoxb8-FlpO mice (Cajal–Retzius cells of the dentate gyrus, and mesenchymal cells of the choroid plexus). While targeted transgenic lines were similar in terms of known spinofugal projections, Hoxb8-IRES-Cre reporters had an additional projection to the core of the facial motor nucleus, and more abundant Hoxb8-lineage microglia scattered throughout the brain than Hoxb8-T2A-FlpO (or any other) mice, suggesting dysregulated Hoxb8-driven reporter expression in one or both lines.DiscussionThis work complements structural and connectivity atlases of the mouse central nervous system, and provides a platform upon which their reactions to injury or disease can be studied. Ectopic Hoxb8-driven recombinase expression may also be a useful tool to study structure and function of other cell populations in non-targeted lines.
Research trends of ferroptosis and pyroptosis in Parkinson’s disease: a bibliometric analysis
Authors: Wu Zihua , Zhong Kexin , Tang Biao , Xie Sijian 
Published: May 2024

ObjectiveThis study aims to visualize the trends and hotspots in the research of “ferroptosis in PD” and “pyroptosis in PD” through bibliometric analysis from the past to 2024.MethodsLiterature was retrieved from the Web of Science Core Collection (WoSCC) from the past to February 16, 2024, and bibliometric analysis was conducted using Vosviewer and Citespace.Results283 and 542 papers were collected in the field of “ferroptosis in PD” and “pyroptosis in PD.” The number of publications in both fields has increased yearly, especially in “ferroptosis in PD,” which will become the focus of PD research. China, the United States and England had extensive exchanges and collaborations in both fields, and more than 60% of the top 10 institutions were from China. In the fields of “ferroptosis in PD” and “pyroptosis in PD,” the University of Melbourne and Nanjing Medical University stood out in terms of publication numbers, citation frequency, and centrality, and the most influential journals were Cell and Nature, respectively. The keyword time zone map showed that molecular mechanisms and neurons were the research hotspots of “ferroptosis in PD” in 2023, while memory and receptor 2 were the research hotspots of “pyroptosis in PD” in 2023, which may predict the future research direction.ConclusionThis study provides insights into the development, collaborations, research themes, hotspots, and tendencies of “ferroptosis in PD” and “pyroptosis in PD.” Overall situation of these fields is available for researchers to further explore the underlying mechanisms and potential treatments.
Prediction of survival after neoadjuvant therapy in locally advanced rectal cancer – a retrospective analysis
Authors: Piringer Gudrun , Ponholzer Florian , Thaler Josef , Bachleitner-Hofmann Thomas , Rumpold Holger , de Vries Alexander , Weiss Lukas , Greil Richard , Gnant Michael , Öfner Dietmar 
Published: May 2024

PurposeThe aim of this retrospective analysis was to determine if the response to preoperative radio(chemo)therapy is predictive for survival among patients with locally advanced rectal cancer and may act as a potential surrogate endpoint for disease free survival and overall survival.ResultsEight hundred seventy-eight patients from five centers were analyzed. There were 304 women and 574 men; the median age was 64.7 years. 77.6% and 22.4% of patients received neoadjuvant radiochemotherapy or short-course radiotherapy, resulting in a pathological complete response in 7.3%. T-downstaging and N-downstaging occurred in 50.5% and 37% of patients after neoadjuvant therapy. In patients with T-downstaging, the 10-year DFS and 10-year OS were 64.8% and 66.8% compared to 37.1% and 45.9% in patients without T-downstaging. N-downstaging resulted in 10-year DFS and 10-year OS in 56.2% and 62.5% compared to 47.3% and 52.3% without N-downstaging. Based on routinely evaluated clinical parameters, an absolute risk prediction calculator was generated for 5-year disease-free survival, and 5-year overall survival.ConclusionT-downstaging and N-downstaging after neoadjuvant radiochemotherapy or short-course radiotherapy resulted in better DFS and OS compared to patients without response. Based on clinical parameters, 5-year DFS, and 5-year OS can be predicted using a prediction calculator.
Predicting treatment failure in stage III colon cancer patients after radical surgery
Authors: Zeng Hao , Zhong Xuejing , Liu Wenxin , Liang Baofeng , Xue Xueyi , Yu Nong , Xu Dongbo , Wang Xiaojie , Lin Shuangming 
Published: May 2024

PurposeThe aim to assess treatment failure in patients with stage III colon cancer who underwent radical surgery and was analyzed using the nomogram.MethodsClinical factors and survival outcomes for stage III colon cancer patients registered in the SEER database from 2018 to 2019 were analyzed, with patients split into training and testing cohorts (7:3 ratio). A total of 360 patients from the First Affiliated Hospital of Longyan served as an external validation cohort. Independent predictors of treatment failure were identified using logistic regression analyses. The nomograms was evaluated by concordance index (C-index), calibration curves, and the area under the curve (AUC), decision curve analysis (DCA) and clinical impact curves (CIC) assessed the clinical utility of nomograms versus TNM staging.ResultsThe study included 4,115 patients with stage III colon cancer. Multivariate logistic analysis age, tumor site, pT stage, pN stage, chemotherapy, pretreatment CEA levels, number of harvested lymph nodes, perineural invasion and marital status were identified as independent risk factors for treatment failure. The C-indices for the training and testing sets were 0.853 and 0.841. Validation by ROC and calibration curves confirmed the stability and reliability of the model. DCA showed that the net clinical effect of the histogram was superior to that of the TNM staging system, while CIC highlighted the potentially large clinical impact of the model.ConclusionsThe developed Nomogram provides a powerful and accurate tool for clinicians to assess the risk of treatment failure after radical surgery in patients with stage III colon cancer.
The etiology and differential diagnosis of “autoimmune hepatitis-like liver disease” in children: a single-center retrospective study
Authors: Ma Di , Liu Xinglou , Ai Guo , Pan Wen , Liu Lingling , Huang Yuan , Liao Yi , Lu Yuanyuan , Zhang Zhan , Zhou Hua , Huang Zhihua , Hao Xingjie , Shu Sainan , Fang Feng 
Published: May 2024

BackgroundChildren with autoimmune hepatitis (AIH) often present with symptoms similar to those of other liver diseases. This study consists of a comparison between the clinical and histological characteristics of AIH and those of other four AIH-like liver diseases [i.e., drug-induced liver injury (DILI), gene deficiency, infectious liver disease and other etiology of liver disease], as well as an evaluation of the AIH scoring system's diagnostic performance.MethodsAll children with AIH-like liver disease at our center from January 2013 to December 2022 were included. The clinical and histological characteristics of the AIH group were retrospectively analyzed and compared with those of the other four groups.ResultsA total of 208 children were included and divided into AIH group (18 patients), DILI group (38 patients), gene deficiency group (44 patients), infectious liver disease group (74 patients), and other etiology group (34 patients). The antinuclear antibodies (ANA) ≥ 1:320 rate was significantly higher in the AIH compared to the other four groups after multiple testing correction (p &lt; 0.0125), while patients with positive antibodies to liver-kidney microsomal-1 (anti-LKM1, n = 3) and smooth muscle antibodies (SMA, n = 2) were only observed in the AIH group. The positive rates of antibodies to liver cytosol type1 (anti-LC1) and Ro52 were higher than those in the other four groups. The serum immunoglobulin G (IgG) and globulin levels, as well as the proportions of portal lymphoplasmacytic infiltration, lobular hepatitis with more than moderate interface hepatitis, and lobular hepatitis with lymphoplasmacytic infiltration, were significantly higher in the AIH group than in the other four groups after multiple testing correction (p &lt; 0.0125). The cirrhosis rate in the AIH group was higher than that in the DILI and infectious liver disease groups (p &lt; 0.0125). Both the simplified (AUC &gt; 0.73) and the revised systems (AUC &gt; 0.93) for AIH have good diagnostic performance, with the latter being superior (p &lt; 0.05).ConclusionPositive autoantibodies (ANA ≥ 1:320 or anti-LKM1 positive, or accompanied by SMA, anti-LC1 or Ro-52 positive) and elevated serum IgG or globulin levels contribute to early recognition of AIH. The presence of lobular hepatitis with more than moderate interface hepatitis and lymphoplasmacytic infiltration contribute to the diagnosis of AIH.
Ricinodendron heudelotii (Euphorbiaceae) seed oil prevents DMBA-induced breast cancer under menopause-like conditions in Wistar rats
Authors: Zingué Stéphane , Tchoupang Edwige Nana , Madji Linda Takou , Pehuie Fomat Boris Hugor , Mafogang Borelle , Njamen Dieudonné , Mendimi Joseph Marie Nkodo 
Published: May 2024

Despite efforts, breast cancer remains associated with a high incidence and mortality rate. Ricinodendron heudelotii also known as “Njansang,” is a plant used for cancer treatment. While several reports on the anticancer potential of its leaves exist, little is known about its seed oil. This study aimed to evaluate the in vitro and in vivo anti-breast cancer activity of “Njansang” seed oil. The inhibitory effect of “Njansang” seed oil was determined using MTT and CCK-8 dye reduction assays. Breast cancer was induced with DMBA and promoted with E2V (1 mg/kg) for 4 weeks in ovariectomized rats (menopausal condition). Evaluated parameters included tumor incidence, tumor mass and volume, histopathology, breast cancer biomarker CA 15–3, antioxidant status (CAT, GSH, MDA, NO, SOD), TNF-α and INFγ levels, lipid profile (total cholesterol, LDL-cholesterol, triglycerides and HDL-cholesterol), as well as toxicity parameters (ALT, AST, creatinine). “Njansang” oil significantly reduced the growth of ER+ (MCF-7) and triple negative (MDA-MB 231) adenocarcinoma cells in vitro as well as tumor incidence, tumor mass and CA 15–3 levels in vivo. It exhibited antioxidant activity, characterized by an increase in SOD and catalase activities, GSH levels and decreased MDA levels compared to the DMBA group. TNF-α and INF-γ levels were reduced following oil treatment, while total cholesterol, LDL-cholesterol and triglyceride levels were reduced. The aforementioned findings confirm the protective effects of “Njansang” oil on induced breast cancer in ovariectomized rats.
Effect of thoracolumbar fascia injury on reported outcomes after percutaneous vertebroplasty
Authors: Yang Songbo , Tang Jie , Yang Zhaoyi , Jin Hongju , Wang Qinglei , Wang Huiming 
Published: May 2024

PurposeThoracolumbar fascia injury is often associated with poor early pain relief after percutaneous vertebroplasty (PVP). This study will evaluate the effects of thoracolumbar fascia injury on early pain relief and time to get out of bed after PVP.MethodsA total of 132 patients treated with PVP for osteoporotic vertebral compression fractures (OVCF) were included and divided into injured group (52 cases) and non-injured group (80 cases) according to the existence of thoracolumbar fascia injury. Before surgery, 1 day, 3 days, 1 week, 1 month, and 3 months after surgery, and at the last follow-up, the primary patient-reported outcome measures (PROMs) were the visual analogue scale (VAS) of pain while rolling over and standing, and the secondary PROMs was the Oswestry disability index (ODI). Meanwhile, the achieved rate of minimal clinically important differences (MCID) and patient acceptable symptom states (PASS) of the above measures in both groups was evaluated at the last follow-up.ResultsExcept for the postoperative 3 months and the last follow-up, there were statistically significant differences in VAS-standing and ODI between the two groups at other time points after surgery (P &lt; 0.05), and the non-injured group was significantly better than the injured group. At the last follow-up, there was no statistically significant difference in the MCID and PASS achievement rates of the above measures between the two groups (P &gt; 0.05). In addition, the proportion of patients who got out of bed 1 and 3 days after surgery in the non-injury group was significantly higher than that in the injury group (P = 0.000 for both).ConclusionThoracolumbar fascia injury significantly affected early pain relief and extended time of getting out of bed after PVP. Attention should be paid to preoperative evaluation of thoracolumbar fascial injury in order to better predict the postoperative efficacy of PVP.
Brand Awareness, Electronic Word-of-Mouth, and Viral Marketing's Effects on Purchase Decisions
Authors: Kinanding* Jalu , Nurrohim Hasa 
Published: May 2024

This research aims to determine and analyze the influence of viral marketing, electronic word of mouth, and brand awareness on purchase decisions. This research is survey research with quantitative approach and data collection tools in the form of questionnaires. The population in this study consists of students in Sleman District, with 100 respondents as the sample using purposive sampling. The data analysis technique used is multiple linear regression with SPSS program. The result of this study show that viral marketing, electronic word of mouth, and brand awareness simultaneously has a positive and significant influence on purchase decisions and viral marketing, electronic word of mouth, and brand awareness partially has a positive and significant influence on purchase decisions. This research contributes to expanding theory and students because it adds references regarding the influence of viral marketing, electronic word of mouth, and brand awareness on purchase decisions at the Shopee Marketplace in Sleman Regency.
Keyword: Viral Marketing; Electronic Word of Mouth; Brand Awareness; Purchase Decision; Shopee
Optimizing Motivation: Leadership Style that Inspires the Work Environment
Authors: Dira Aldi Friyatna , Sawitri Ina , Abdulrahman Abdulrahman , Utomo* Kurniawan Prambudi 
Published: May 2024

The purpose of the research is to determine the influence of each variable of leadership style and work environment on motivation, as well as to optimize these variables in order to enhance employee motivation. This research method employs quantitative descriptive analysis using SPSS V.26 on the results from 40 respondents. The findings from the employees of the Transportation Department and the community as users of related transportation services indicate that the variables of the work environment’s role and leadership style have a significant positive impact on employee motivation. Therefore, it is recommended that the Transportation Department (Dishub) of Bekasi City should continue to maintain a harmonious relationship between leaders and employees to improve performance, thereby producing effective and adequate transportation services to increase the satisfaction of the Bekasi City community as users of transportation services.
Keyword: Optimization; Work Environment; Leadership Style; Motivation; Employees
Capital Market Reaction to the Strengthening of the Rupiah Exchange Rate against the US Dollar Exchange Rate
Authors: Lubis* M Zaky Mubarak , Putra Gusti Dirga Alfakhri , Binti Mohamad Zam Zuriyati 
Published: May 2024

The purpose of this study is to analyze the response of Indonesian capital markets to differences in average abnormal returns and average trading volume activity before and after the strengthening of the IDR/USD exchange rate. This study is a quantitative study using the event study method. The sample used consists of the stocks included in the LQ45 index. the most active stocks with a large daily trading volume. Hypothesis testing and paired t-test. The results of the hypothesis test show that: there is no significant difference in the average abnormal returns before and after the strengthening of the rupiah against the US dollar because are sig. (2-tailed) value of 0.417 > 0.05; there is no significant difference in the average trading volume activity before and after the strengthening of the rupiah against the US dollar because sig. (2-tailed) value of 0.080 >0.05.
Keyword: Market Reaction; Event Study; Abnormal Return; Trading Volume Activity; Exchange Rate
The Worth of Islamic Piety for High-Quality Work Performed by Employees without Fraud
Authors: Ilma Robbi Zidni 
Published: May 2024

Employees are the most important aspect of every organization, whose success can be determined by its employees whether they have good work quality or not. Good work quality is supported by performance without Fraud. This study aims to determine the role of an employee's Islamic piety value in work quality without Fraud. This is a work with a qualitative research method with an exploratory writing style. Selection of informants using purposive sampling. The primary data presented comes from in-depth and literature study as secondary data. Test the validity of the data as stated in the Triangulation of Sources and Theories technique. For Islamic people, piety is very much related to Fraud prevention, and also every pious employee will implement the teachings of his religion to be professional (not cheating). This research provides an overview to the stakeholders of each organization to find out other aspects in optimizing the quality of work of its employees.
Keyword: Syariah Economy; Management; Employees; Work Quality; Fraud
Hubungan Kepuasan Kerja Dengan Turnover Intention Pada Perawat Pegawai Negeri Sipil Dan Tenaga Kontrak Di Rumah Sakit Umum Daerah Talisayan
Authors: Mukaromah Siti , Muhadi Muhadi 
Published: May 2024

Niat berhenti turnover intention pegawai yang tinggi adalah ukuran yang umum digunakan untuk mengukur permasalahan mendasar suatu organisasi. Kepuasan kerja merupakan satu diantara beberapa penyebab turnover intention dimana pegawai merasa tidak puas dengan berbagai aspek seperti pendapatan, beban pekerjaan, pengawasan dll. Penelitian ini adalah penelitian deskriptif analitik, yang dilakukan untuk mengetahui bagaimana hubungan kepuasan kerja dengan turnover intention perawat Pegawai Negeri Sipil dan Tenaga kontrak di RSUD Talisayan, dilakukan dengan pendekatan crosssectional. Responden yang dilibatkan adalah seluruh perawat dengan jumlah 39 orang, dilaksanakan bulan November sd Desember 2023. Cara pengambilan data melalui kuisioner yang terdiri dari kepuasan kerja dan turnover intention. Untuk pengolahan data menggunakan uji chi-square SPSS 23 dengan nilai signifikansi kurang 0.05. Hasil penelitian menunjukkan bahwa secara umum perawat merasa puas dan keinginan untuk meninggalkan pekerjaannya cukup rendah, diketahui dari variabel kepuasan kerja yang tidak puas 18 perawat (46,15%) puas 21 perawat 53,84% dan untuk variabel turnover intention tinggi ada 16 perawat 41,02% rendah 23 perawat 67,65%. Hasil uji crosstabs menunjukkkan nilai asymp.sig 0.00 atau p kurang 0.05 maka dapat disimpulkan Ho ditolak dan Ha diterima atau terdapat hubungan yang signifikan antara kepuasan kerja dengan turnover intention pada perawat PNS dan tenaga kontrak di RSUD Talisayan. Dalam penelitian ini saran yang dapat disampaikan pada pihak manajemen dalam upaya mencegah peningkatan turnover intention di RSUD Talisayan sebaiknya membuka ruang diskusi dengan pegawai sehingga jika ada keinginan atau harapan juga permasalahan dari pegawai dapat segera tersampaikan dan diupayakan untuk dicarikan solusinya.
Highly Efficient Clay Based Degumming Aid for Phosphorous Removal from Crude Palm Oil
Authors: Bajpai Indu , Pal Pawan , Bhasin Sanjeev 
Published: May 2024

The removal of phosphorous from crude oil is a complex and necessary step to enhance the oxidative stability and shelf life of edible oil. In this aspect, the current work proposed clay based degumming aid for the removal of phosphorous from the crude palm oil during the bleaching process. The state of art qualitative and quantitative techniques was adopted for the study of physical and chemical properties of different raw materials and oil. A profound study on the stability of the degumming aid is based on the assessment of phosphorous removal efficiency with time (up to 6 months) and bleachability. The results of current work revealed that clay based degumming aid is highly effective in removing phosphorous up to 96%. Additionally, the bleaching performance of clay-based aid is also palpable (around 45%), which influences the deodorization process of the crude palm oil positively. The clay based degumming aid eliminate separate degumming process with acids, which directly impact the oil processing time and cost. Overall, proposed clay based degumming aid is highly efficient, stable for long time and cost effective as well, for the removal of undesired phosphorous from the crude palm oil during the bleaching process.