bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in physicochemical properties of pectin extracted from pomelo peel with different extraction techniques
Authors: Yu Yangyang , Lu Ping , Yang Yongfeng , Ji Huifu , Zhou Hang , Chen Siyuan , Qiu Yao , Chen Hongli 
Published: April 2024

AbstractIn order to obtain high yield pomelo peel pectin with better physicochemical properties, four pectin extraction methods, including hot acid extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction, and enzymatic assisted extraction (EAE) were compared. MAE led to the highest pectin yield (20.43%), and the lowest pectin recovery was found for EAE (11.94%). The physicochemical properties of pomelo peel pectin obtained by different methods were also significantly different. Pectin samples obtained by MAE had the highest methoxyl content (8.35%), galacturonic acid content (71.36%), and showed a higher apparent viscosity, thermal and emulsion stability. The pectin extracted by EAE showed the highest total phenolic content (12.86%) and lowest particle size (843.69 nm), showing higher DPPH and ABTS scavenging activities than other extract methods. The pectin extracted by HAE had the highest particle size (966.12 nm) and degree of esterification (55.67%). However, Fourier-transform infrared spectroscopy showed that no significant difference occurred among the different methods in the chemical structure of the extracted pectin. This study provides a theoretical basis for the industrial production of pomelo peel pectin.
A novel indicator to predict the outcome of percutaneous stereotactic radiofrequency rhizotomy for trigeminal neuralgia patients: diffusivity metrics of MR-DTI
Authors: Su Xu , Wang Zhengming , Wang Zhijia , Cheng Min , Du Chao , Tian Yu 
Published: April 2024

AbstractMagnetic resonance-diffusion tensor imaging (MR-DTI) has been used in the microvascular decompression and gamma knife radiosurgery in trigeminal neuralgia (TN) patients; however, use of percutaneous stereotactic radiofrequency rhizotomy (PSR) to target an abnormal trigeminal ganglion (ab-TG) is unreported. Fractional anisotropy (FA), mean and radial diffusivity (MD and RD, respectively), and axial diffusivity (AD) of the trigeminal nerve (CNV) were measured in 20 TN patients and 40 healthy control participants immediately post PSR, at 6-months, and at 1 year. Longitudinal alteration of the diffusivity metrics and any correlation with treatment effects, or prognoses, were analyzed. In the TN group, either low FA (value < 0.30) or a decreased range compared to the adjacent FA (dFA) > 17% defined an ab-TG. Two-to-three days post PSR, all 15 patients reported decreased pain scores with increased FA at the ab-TG (P < 0.001), but decreased MD and RD (P < 0.01 each). Treatment remained effective in 10 of 14 patients (71.4%) and 8 of 12 patients (66.7%) at the 6-month and 1-year follow-ups, respectively. In patients with ab-TGs, there was a significant difference in treatment outcomes between patients with low FA values (9 of 10; 90%) and patients with dFA (2 of 5; 40%) (P < 0.05). MR-DTI with diffusivity metrics correlated microstructural CNV abnormalities with PSR outcomes. Of all the diffusivity metrics, FA could be considered a novel objective quantitative indicator of treatment effects and a potential indicator of PSR effectiveness in TN patients.
Study on dynamic response and long-term settlement of silty soil around Shanghai metro tunnel
Authors: Sang Biaowei , Yan Chunling , Wang Cheng , Qu Xin 
Published: April 2024

AbstractAt present, the method for calculating long-term tunnel settlement predictions under metro loading considers only one working condition of passenger loading, which is inconsistent with actual working conditions. To establish a tunnel settlement model that accounts for variations in passenger flow, this study uses data mining methods to categorize metro operation into three working conditions: "peak period, secondary period, and low period." The impact of these passenger flow conditions on the dynamic response of the soil around the tunnel is analyzed. Then, based on the principles of calculus, a calculus-based prediction model is established to consider the changing patterns of metro passenger flow. The model is applied to analyze the long-term settlement characteristics of Shanghai Metro Line 10. The results indicate that, under identical conditions, soil displacement and dynamic deviatoric stress around the tunnel increase with passenger capacity. The calculus prediction model aligns more closely with actual working conditions than the conventional model. The predicted tunnel settlement of Shanghai Metro Line 10 after 20 years of operation is approximately 37.07 mm, with most settlement occurring in the early stages, primarily due to cumulative plastic deformation of the soil.
Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections
Authors: Lerdsittikul Varintip , Apiratwarrasakul Sukanya , Atithep Thassanant , Withatanung Patoo , Indrawattana Nitaya , Pumirat Pornpan , Chaiwattanarungruengpaisan Somjit , Thongdee Metawee 
Published: April 2024

AbstractThe increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) emphasises the urgent need for novel antimicrobial agents as alternatives to antibiotics. Bacteriophage therapy is one of the most promising antimicrobial strategies. Here, we isolated and comprehensively characterized a novel Staphylococcus phage, vB_SauM_VL10 (VL10), from urban sewage. The VL10 genome displays 141,746 bp of linear double-stranded DNA, containing 193 open reading frames and lacking tRNA, virulence, or antibiotic resistance genes. Phylogenetic analysis categorizes VL10 as a novel species within the Silviavirus genus, Twortvirinae subfamily. VL10 exhibits lytic behaviour characterized by efficient adsorption, a short latent period, and substantial burst size, with environmental stability. It demonstrates lytic activity against 79.06% of tested S. aureus strains, highlighting its species specificity. Additionally, VL10 effectively targets MRSA biofilms, reducing biomass and viable cells. In MRSA-infected G. mellonella larvae, VL10 enhances survival rates, supporting its potential for phage therapy applications. Moreover, the emergence of VL10-resistant S. aureus strains associated with fitness trade-offs, including reduced growth, biofilm formation, and virulence. Altogether, these findings emphasize VL10 as a promising candidate for developing therapeutic agents against MRSA infections, providing insights into phage biology and resistance dynamics.
Oxidative stress, inflammation, and steatosis elucidate the complex dynamics of HgCl2 induced liver damage in Channa punctata
Authors: Singh Shefalee , Dwivedi Shikha , Khan Adeel Ahmad , Jain Anamika , Dwivedi Shraddha , Yadav Kamlesh Kumar , Dubey Indrani , Trivedi Abha , Trivedi Sunil P. , Kumar Manoj 
Published: April 2024

AbstractWater bodies are highly pollution-prone areas in which mercury (Hg) is considered as a major menace to aquatic organisms. However, the information about the toxicity of mercuric chloride (HgCl2) in a vital organ such as the liver of fish is still inadequate. This study aimed to assess the impact of mercuric chloride (HgCl2) exposure on the liver of Channa punctata fish over 15, 30, and 45 days, at two different concentrations (0.039 mg/L and 0.078 mg/L). Mercury is known to be a significant threat to aquatic life, and yet, information regarding its effects on fish liver remains limited. The results of this study demonstrate that exposure to HgCl2 significantly increases oxidative stress markers, such as lipid peroxidation (LPO) and protein carbonyls (PC), as well as the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) in the fish. Additionally, the transcriptional and protein analysis of specific genes and molecules associated with necroptosis and inflammation, such as ABCG2, TNF α, Caspase 3, RIPK 3, IL-1β, Caspase-1, IL-18, and RIPK1, confirm the occurrence of necroptosis and inflammation in the liver. Histopathological and ultrastructural examinations of the liver tissue further reveal a significant presence of liver steatosis. Interestingly, the upregulation of PPARα suggests that the fish's body is actively responding to counteract the effects of liver steatosis. This study provides a comprehensive analysis of oxidative stress, biochemical changes, gene expression, protein profiles, and histological findings in the liver tissue of fish exposed to mercury pollution in freshwater environments.
Enhancing accuracy and convenience of golf swing tracking with a wrist-worn single inertial sensor
Authors: Kim Myeongsub , Park Sukyung 
Published: April 2024

AbstractIn this study, we address two technical challenges to enhance golf swing trajectory accuracy using a wrist-worn inertial sensor: orientation estimation and drift error mitigation. We extrapolated consistent sensor orientation from specific address-phase signal segments and trained the estimation with a convolutional neural network. We then mitigated drift error by applying a constraint on wrist speed at the address, backswing top, and finish, and ensuring that the wrist's finish displacement aligns with a virtual circle on the 3D swing plane. To verify the proposed methods, we gathered data from twenty male right-handed golfers, including professionals and amateurs, using a driver and a 7-iron. The orientation estimation error was about 60% of the baseline, comparable to studies requiring additional sensor information or calibration poses. The drift error was halved and the single-inertial-sensor tracking performance across all swing phases was about 17 cm, on par with multimodal approaches. This study introduces a novel signal processing method for tracking rapid, wide-ranging motions, such as a golf swing, while maintaining user convenience. Our results could impact the burgeoning field of daily motion monitoring for health care, especially with the increasing prevalence of wearable devices like smartwatches.
Experimental study and machine learning modeling of water removal efficiency from crude oil using demulsifier
Authors: Hashem H. H. , Kikhavani T. , Moradkhani M. A. 
Published: April 2024

AbstractThis study deals with the investigation of the water removal efficiency (WRE) from crude oil using a commercial demulsifier. The impacts of time, demulsifier concentration, and temperature on WRE were experimentally studied. The results implied the fact that temperature plays a substantial role in the demulsification and has a direct correlation with WRE. In addition, while increasing the concentration up to 40 ppm contributed to reaching a higher WRE, it did not have positive effects on efficiency at higher concentrations (overdose) and just led to more demulsifier consumption. The concentration dependence of WRE was also diminished at high temperatures. At higher levels of temperature and concentration, the time required to reach a high WRE was noticeably reduced. In order to generalize the findings of this study, the measured experimental data were employed to design predictive methods for WRE based on two smart soft-computing paradigms, including Multilayer perceptron (MLP) and Gaussian process regression (GPR). Despite the high accuracy of both models, the MLP model presented the best consistencies with experimental data with average absolute relative error and relative root mean squared error of 0.84%, and 0.01%, respectively during the testing (validation) step. Also, a visual description through the contour diagram confirmed the capability of the recently proposed models to describe the physical variations of WRE under various operating conditions. Ultimately, a sensitivity analysis based on the MLP model was undertaken to shed light on the order of significance of operational factors in controlling WRE. Overall, the findings of the current research, in turn, have a satisfactory contribution to the efficient design of the water removal process from crude oil based on demulsifiers.
The influence of AI on the economic growth of different regions in China
Authors: Lin Shuang , Wang Minke , Jing Chongyi , Zhang Shengda , Chen Jiuhao , Liu Rui 
Published: April 2024

AbstractHigh-quality development plays a crucial role in China’s economic progress in the new era. It represents a new concept of advancement and mirrors the increasing aspirations of the populace for an improved standard of living. In this context, the role of artificial intelligence (AI) in promoting sustainable development cannot be overemphasized. This paper explores how AI technologies can drive the transition to a green, low-carbon and circular economy. We have established an index system to measure the development level of the artificial intelligence industry and the high-quality development of the economy, which is relevant to the current state of the artificial intelligence industry and the advancement of the economy. Panel data from 2008 to 2017 has been utilized for this purpose. Global principal component analysis method and entropy value method are employed in the evaluation. Through in-depth analysis of the application of artificial intelligence and environmental protection in various provinces and cities, we clarify that artificial intelligence promotes innovation, saves resources, and is conducive to the development of green economy in the new era.
The agreement between jugular bulb and cerebrospinal fluid lactate levels in patients with out-of-hospital cardiac arrest
Authors: Park Jung Soo , You Yeonho , Kang Changshin , Jeong Wonjoon , Ahn Hong Joon , Min Jin Hong , In Yong Nam , Jeon So Young 
Published: April 2024

AbstractWe investigated the agreement between the jugular bulb (JB) and cerebrospinal fluid (CSF) lactate levels. The study was conducted from July 2021 to June 2023 as a prospective observational cohort study at a single center. The right jugular vein was accessed, and the placement of JB catheter tip was confirmed using lateral cervical spine X-ray. A lumbar catheter was inserted between the 3rd and 4th lumbar spine of the patient. Lactate levels were measured immediately, 24 h, 48 h, and 72 h after ROSC. In patients with a good neurological prognosis, kappa between JB and CSF lactate levels measured immediately, at 24 h, 48 h, and 72 h after ROSC were 0.08, 0.36, 0.14, − 0.05 (p = 0.65, 0.06, 0.48, and 0.75, respectively). However, in patients with a poor neurological prognosis, kappa between JB and CSF lactate levels measured immediately, at 24 h, 48 h, and 72 h after ROSC were 0.38, 0.21, 0.22, 0.12 (p = 0.001, 0.04, 0.04, and 0.27, respectively). This study demonstrated that JB lactate levels exhibited significant agreement with arterial lactate levels, compared to CSF lactate levels. Therefore, this should be considered when using JB lactate to monitor cerebral metabolism.
Exercise instruction during haemodialysis treatment after changes to the insurance regime: a nationwide questionnaire survey in Japan
Authors: Sofue Tadashi , Matsuzawa Ryota , Nishiwaki Hiroki , Tsuchida Yohei , Kosaki Keisei , Hoshino Junichi , Narita Ichiei , Yamagata Kunihiro 
Published: April 2024

AbstractIn April 2022, an additional medical fee for exercise instruction during haemodialysis treatment was approved for insurance claims in Japan. We conducted a questionnaire survey to investigate the current situation regarding exercise therapy during haemodialysis treatment after this change. Questionnaires were mailed to 4257 haemodialysis facilities, almost all the haemodialysis facilities in Japan, on January 31, 2023. In total, 1657 facilities responded, of which 550 (33%) provided exercise instruction during haemodialysis treatment, and 65% of these claimed the new fee. Of the 550 facilities that had claimed the fee at the time of survey, 245 (55%) started exercise instruction in April 2022 or later. Exercise instruction focused on resistance training (81%) and aerobic exercise (62%) for 20–30 min (66%) three times a week (80%). The instructors included physicians in 45% of facilities, nurses in 74%, and physical therapists in 36%. Efficacy was evaluated in 76% of the facilities providing instruction, mainly by assessing change in muscle strength (49%). Overall, 39% of facilities had experienced some adverse events, but none were life-threatening. In conclusion, after the change in the insurance regime, exercise instruction during haemodialysis treatment has become more popular, and more patients on haemodialysis are undergoing exercise therapy.
Discovery of gefitinib-1,2,3-triazole derivatives against lung cancer via inducing apoptosis and inhibiting the colony formation
Authors: Gao En , Wang Ya , Fan Gao-lu , Xu Guiqing , Wu Zi-Yuan , Liu Zi-Jun , Liu Jian-Cheng , Mao Long-Fei , Hou Xixi , Li Shouhu 
Published: April 2024

AbstractA series of 20 novel gefitinib derivatives incorporating the 1,2,3-triazole moiety were designed and synthesized. The synthesized compounds were evaluated for their potential anticancer activity against EGFR wild-type human non-small cell lung cancer cells (NCI-H1299, A549) and human lung adenocarcinoma cells (NCI-H1437) as non-small cell lung cancer. In comparison to gefitinib, Initial biological assessments revealed that several compounds exhibited potent anti-proliferative activity against these cancer cell lines. Notably, compounds 7a and 7j demonstrated the most pronounced effects, with an IC50 value of 3.94 ± 0.17 µmol L−1 (NCI-H1299), 3.16 ± 0.11 µmol L−1 (A549), and 1.83 ± 0.13 µmol L−1 (NCI-H1437) for 7a, and an IC50 value of 3.84 ± 0.22 µmol L−1 (NCI-H1299), 3.86 ± 0.38 µmol L−1 (A549), and 1.69 ± 0.25 µmol L−1 (NCI-H1437) for 7j. These two compounds could inhibit the colony formation and migration ability of H1299 cells, and induce apoptosis in H1299 cells. Acute toxicity experiments on mice demonstrated that compound 7a exhibited low toxicity in mice. Based on these results, it is proposed that 7a and 7j could potentially be developed as novel drugs for the treatment of lung cancer.
Mitochondrial enzyme FAHD1 reduces ROS in osteosarcoma
Authors: Heberle Anne , Cappuccio Elia , Andric Andreas , Kuen Tatjana , Simonini Anna , Weiss Alexander K. H. 
Published: April 2024

AbstractThis study investigated the impact of overexpressing the mitochondrial enzyme Fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) in human osteosarcoma epithelial cells (U2OS) in vitro. While the downregulation or knockdown of FAHD1 has been extensively researched in various cell types, this study aimed to pioneer the exploration of how increased catalytic activity of human FAHD1 isoform 1 (hFAHD1.1) affects human cell metabolism. Our hypothesis posited that elevation in FAHD1 activity would lead to depletion of mitochondrial oxaloacetate levels. This depletion could potentially result in a decrease in the flux of the tricarboxylic acid (TCA) cycle, thereby accompanied by reduced ROS production. In addition to hFAHD1.1 overexpression, stable U2OS cell lines were established overexpressing a catalytically enhanced variant (T192S) and a loss-of-function variant (K123A) of hFAHD1. It is noteworthy that homologs of the T192S variant are present in animals exhibiting increased resistance to oxidative stress and cancer. Our findings demonstrate that heightened activity of the mitochondrial enzyme FAHD1 decreases cellular ROS levels in U2OS cells. However, these results also prompt a series of intriguing questions regarding the potential role of FAHD1 in mitochondrial metabolism and cellular development.
Formation mechanisms and environmental influences on the crystal growth of wulfenite
Authors: Gračanin Nik , Podlogar Matejka , Semsari Parapari Sorour , Boulet Pascal , Ruiz-Zepeda Francisco , Šturm Sašo , Rogan Šmuc Nastja 
Published: April 2024

AbstractIn this study, we introduce a novel approach using correlative analysis techniques to unravel detailed insights into the environmental influences on crystal growth. Tabular and bipyramidal wulfenite samples from the Mežica mine in north-eastern Slovenia were analysed to combine the morphological aspects of crystal growth with the atomic-resolution reconstruction of the positions of lead (Pb) and molybdenum (Mo) atoms in the parent crystal lattice. These combined data also allow us to present the formation mechanism that enables the development of bipyramidal or tabular morphologies in wulfenite. The bipyramidal and tabular crystals are chemically pure wulfenite (PbMoO4), as confirmed by various advanced diffraction and spectroscopy techniques. However, each habit includes multiple inclusions, mostly consisting of carbonates, Pb-Fe oxides, Pb oxides and, more rarely, Pb vanadate (descloizite). The differences in the morphologies can be attributed to compositional changes during precipitation from a meteoric solution and thus, we propose a growth mechanism consisting of three different phases of growth. This innovative approach emphasises the importance of understanding the origin of crystal habits, as can help to decipher how external influences can affect the crystal structure and its surface, leading to the dissolution of preferred surfaces and the selective release of Pb and Mo.
Iron status and sarcopenia-related traits: a bi-directional Mendelian randomization study
Authors: Chen Honggu , Zhang Ziyi , Wang Yizhe , Ma Anpei , Li Lingbo , Zhao Guoyang 
Published: April 2024

AbstractAlthough serum iron status and sarcopenia are closely linked, the presence of comprehensive evidence to establish a causal relationship between them remains insufficient. The objective of this study is to employ Mendelian randomization techniques to clarify the association between serum iron status and sarcopenia. We conducted a bi-directional Mendelian randomization (MR) analysis to investigate the potential causal relationship between iron status and sarcopenia. MR analyses were performed using inverse variance weighted (IVW), MR-Egger, and weighted median methods. Additionally, sensitivity analyses were conducted to verify the reliability of the causal association results. Then, we harvested a combination of SNPs as an integrated proxy for iron status to perform a MVMR analysis based on IVW MVMR model. UVMR analyses based on IVW method identified causal effect of ferritin on appendicular lean mass (ALM, β = − 0.051, 95% CI − 0.072, − 0.031, p = 7.325 × 10–07). Sensitivity analyses did not detect pleiotropic effects or result fluctuation by outlying SNPs in the effect estimates of four iron status on sarcopenia-related traits. After adjusting for PA, the analysis still revealed that each standard deviation higher genetically predicted ferritin was associated with lower ALM (β = − 0.054, 95% CI − 0.092, − 0.015, p = 0.006). Further, MVMR analyses determined a predominant role of ferritin (β = − 0.068, 95% CI − 0.12, − 0.017, p = 9.658 × 10–03) in the associations of iron status with ALM. Our study revealed a causal association between serum iron status and sarcopenia, with ferritin playing a key role in this relationship. These findings contribute to our understanding of the complex interplay between iron metabolism and muscle health.