bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sorption of ortho‐Phenylphenol to Soils
Authors: Zheng Chunli , Focks Andreas , Ellebrake Katrin , Eggers Till , Fries Elke 
Published: January 2011

AbstractConflicting sorption coefficients for ortho‐phenylphenol (OPP) have been reported in the literatures, which resulted in the conflicting assessments on OPP mobility in soil. To ascertain the sorption coefficient of OPP, batch experiments were performed based on OECD guideline 106, using three types of soils. Headspace solid‐phase microextraction (HS‐SPME) and GC‐MS were applied to the determination of OPP concentration in the liquid phase. The sorption isotherms obtained for all three soils under equilibrium conditions were described well, assuming linear sorption. The organic carbon normalized distribution coefficients (Koc) ranged from 894 to 1703 L kg−1, which suggested that OPP is moderately mobile in soil. The results also showed that the Koc value of OPP can be predicted precisely from Kow, whereas it was underestimated by one order of magnitude when water solubility is used.
Combinatorial Pharmacologic Effects of Gemcitabine and its Metabolite dFdU
Authors: Benyumov Alexey , Gurvich Vadim J. , Lis Lev G. , Williams Brent W. , Kirstein Mark N. 
Published: January 2011

AbstractRecent evidence has shown that the gemcitabine metabolite, dFdU, is pharmacologically active. Though less potent, dFdU has a longer half‐life and could potentiate or antagonize the activity of gemcitabine. Hence, studies were undertaken to evaluate the combined effects. Following chemical synthesis, an improved purification procedure for dFdU was developed (80 % yield; >99 % purity). Zebrafish phenotype‐based embryo screens revealed no acute toxicity after gemcitabine or dFdU treatment. Only gemcitabine affected zebrafish development in a dose‐dependent manner. Synergy or antagonism for the combination was not observed. Antitumor effects for dFdU were dose dependent. Antagonism was tumor cell‐line dependent and did not depend on formation of the intracellular active metabolite of gemcitabine, suggesting that the drug–metabolite interaction occurs later. These studies highlight a platform for testing the pharmacologic activity for anticancer agent and metabolite combinations. Such analyses are expected to provide insight into the beneficial or harmful effect(s) of metabolites towards parent drug activity.
Improved Temporal Resolution and Linked Hidden Markov Modeling for Switchable Single‐Molecule FRET
Authors: Uphoff Stephan , Gryte Kristofer , Evans Geraint , Kapanidis Achillefs N. 
Published: January 2011

AbstractSwitchable FRET is the combination of single‐molecule Förster resonance energy transfer (smFRET) with photoswitching, the reversible activation and deactivation of fluorophores by light. By photoswitching, multiple donor–acceptor fluorophore pairs can be probed sequentially, thus allowing observation of multiple distances within a single immobilized molecule. Control of the photoinduced switching rates permits adjustment of the temporal resolution of switchable FRET over a wide range of timescales, thereby facilitating application to various dynamical biological systems. We show that fast total internal reflection (TIRF) microscopy can achieve measurements of two FRET pairs with 10 ms temporal resolution within less than 2 s. The concept of switchable FRET is also compatible with confocal microscopy on immobilized molecules, providing better data quality at high temporal resolution. To identify states and extract their transitions from switchable FRET time traces, we also develop linked hidden Markov modeling (HMM) of both FRET and donor–acceptor stoichiometry. Linked HMM successfully identifies transient states in the two‐dimensional FRET–stoichiometry space and reconstructs their connectivity network. Improved temporal resolution and novel data analysis make switchable FRET a valuable tool in molecular and structural biology.
DEC‐205 mediates antigen uptake and presentation by both resting and activated human plasmacytoid dendritic cells
Authors: Tel Jurjen , Benitez‐Ribas Daniel , Hoosemans Sander , Cambi Alessandra , Adema Gosse J. , Figdor Carl G. , Tacken Paul J. , de Vries I. Jolanda M. 
Published: January 2011

AbstractDEC‐205 is a type I C‐type lectin receptor (CLR) that is expressed on various APC subsets and has been suggested to bind necrotic and apoptotic cells. Here we study DEC‐205 characteristics in plasmacytoid DCs (pDCs) obtained from healthy individuals and assess its ability to mediate antigen presentation by isolating sufficient numbers of pDCs from apheresis material obtained from stage III/IV melanoma patients. The results demonstrate that DEC‐205 is expressed on human pDCs. Internalization of DEC‐205 after antibody ligation is clathrin‐ and dynamin‐dependent as it is blocked by hypertonic shock or by inhibition of dynamin activity. Antibody targeting to DEC‐205 does not affect TLR‐induced expression levels of co‐stimulatory and MHC molecules, but clearly impairs TLR‐induced IFN‐α secretion by 40%. We observed that TLR‐mediated signaling increases DEC‐205 expression levels without affecting receptor internalization. Moreover, human pDCs retained the capacity to present antigens via DEC‐205 following TLR activation.
Hyaluronan binding identifies the most proliferative activated and memory T cells
Authors: Maeshima Nina , Poon Grace F. T. , Dosanjh Manisha , Felberg Jackie , Lee Sally S. M. , Cross Jennifer L. , Birkenhead Darlene , Johnson Pauline 
Published: January 2011

AbstractCD44 is expressed on T cells where its ability to bind hyaluronan is tightly regulated. Here, we investigated when T cells bind hyaluronan during an immune response. We found that naïve, murine T cells do not bind fluoresceinated hyaluronan but are induced to bind upon antigen‐induced T‐cell activation in vitro and in vivo. Hyaluronan binding occurred on proliferating T cells and the percentage of hyaluronan‐binding cells correlated with the strength of the activation stimulus. A small percentage of hyaluronan‐binding cells persisted after in vitro activation and had a memory phenotype (CD122+CD44hi). This hyaluronan‐binding population increased after culture with IL‐7 or IL‐15 and proliferated more rapidly than nonbinding cells. In vivo, approximately 20–30% of antigen‐specific OT‐I CD8+ memory T cells in the spleen and BM bound hyaluronan. Hyaluronan binding identified memory cells that proliferated faster in IL‐7 and IL‐15, and enriched for CD62L+ central memory cells. In vivo homeostatic proliferation induced hyaluronan binding on a small percentage of the most rapidly dividing cells after several cell divisions. This study demonstrates that hyaluronan binding is induced upon antigen‐induced T‐cell activation and occurs on a percentage of the most proliferative activated and memory T cells.
HIV interferes with SOCS‐1 and ‐3 expression levels driving immune activation
Authors: Miller Regina C. , Schlaepfer Erika , Baenziger Stefan , Crameri Reto , Zeller Sabine , Byland Rahel , Audigé Annette , Nadal David , Speck Roberto F. 
Published: January 2011

AbstractHIV infection is characterized by sustained immune activation, which is reflected by activated T cells and, in particular, by increased levels of phosphorylated STAT proteins. Here, we hypothesized that T‐cell activation in HIV infection is partially due to the inability of SOCS‐1 and SOCS‐3 to control the JAK/STAT pathway. We found higher levels of SOCS‐1/3 mRNA levels in CD4+ T cells of HIV‐infected patients than in healthy controls. However, SOCS protein levels were lower, explaining the lack of attenuation of the JAK/STAT pathway. Infection of CD4+ T cells alone did not activate STATs, while ex vivo infection of PBMC did, indicating that non‐T cells critical for shaping the immune response, e.g. DC were responsible for the STAT‐1 activation. Supernatants from ex vivo‐infected PBMC transferred to CD4+ T cells induced JAK/STAT activation, pointing to a central role of soluble factors. Notably, over‐expression of SOCS‐1/3 in CD4+ T cells prevented JAK/STAT activation. Thus, HIV infection interferes with SOCS‐1/3 expression driving immune activation. Sustained immune activation disrupts the lymphoid system and favors HIV replication since HIV preferentially infects activated cells. We speculate that regulating SOCS may be a potential way to counteract immune activation in HIV disease.
Molybdenum and Tungsten Oxidoreductase Models
Authors: Schulzke Carola 
Published: January 2011

AbstractThis microreview gives an overview about advances in bioinorganic model chemistry for molybdenum‐ and tungsten‐dependent oxidoreductases with respect to specific aspects of the evolution, structure or function of distinct active‐site properties. The approaches to evaluate catalytic properties fine‐tuned by the ligand sphere, interpret and assign enzyme spectra and to understand why two different metals are used for the same task but in distinct habitats and respective evolutionary aspects are discussed on the basis of exemplary case studies. This short review provides a personal perspective and appreciation of the complex problems associated with the respective foremost synthetic and analytical model chemistry and their solutions partly accompanied and supported by theoretical results.
Structural, Magnetic and Dynamic Characterization of Liquid Crystalline Iron(III) Schiff Base Complexes with Asymmetric Ligands
Authors: Domracheva Natalia , Pyataev Andrew , Manapov Rafil , Gruzdev Matvey , Chervonova Ulyana , Kolker Arkadij 
Published: January 2011

AbstractThe iron(III) complexes that were formed by coordination of the FeIII ion with the asymmetric tridentate liquid crystalline Schiff base ligand (L), the water molecules and the different counterions [PF6– (1), NO3– (2), and Cl– (3)] were studied by electron paramagnetic resonance (EPR) spectroscopy. EPR spectroscopy demonstrated that each of the complexes investigated consists of two types of iron centers: S = 1/2 low‐spin (LS) and S = 5/2 high‐spin (HS). LS iron complexes 2, 3 and LS complex 1 in the temperature range 4.2–250 K have a (dxz,dyz)4(dxy)1 ground state. Interesting features werefound for the monocationic FeIII complex 1, [Fe(L)X(H2O)2]+X–, with X = PF6– as the counterion. The LS and HS iron centers of 1 are coupled together antiferromagnetically and form a dimer structure by means of the water molecules and the PF6– counterion. The second‐type of LS and HS centers that are visible by means of EPR spectroscopy were best observed in the liquid crystalline (387–405 K) phase. The monitoring and the simulation of the EPR spectra enabled us to trace the dynamics of changing the number of the second‐type of LS centers with respect to the first‐type of LS centers. The observed dynamic process is characterized by the enthalpy value ΔH = 27.9 kJ/mol, which was caused by reorientation of the PF6– counterion. Calculation of the observed g values for the second‐type of LS complex 1 indicated that, in this case, the (dxy)2(dxz,dyz)3 ground state is stabilized. The conversion between the electron (dxz,dyz)4(dxy)1/(dxy)2(dxz,dyz)3 configurations was found to be temperature dependent and was detected in the same material for the first time in iron complexes.
C–H Activation in Diiron Bridging Vinyliminium Ligands: Reaction with CS2 to Form New Zwitterionic Complexes Acting as Organometallic Ligands
Authors: Marchetti Fabio , Zacchini Stefano , Salmi Mauro , Busetto Luigi , Zanotti Valerio 
Published: January 2011

AbstractThe reactions of vinyliminium complexes [Fe2{μ‐η1:η3‐C(R′)=C(H)C=N(Me)(R)}(μ‐CO)(CO)(Cp)2][SO3CF3] (R = Xyl, R′ = Me, 1a; R = Xyl, R′ = Tol, 1b; R = Xyl, R′ = COOMe, 1c; R = Me, R′ = Me, 1d; R = Me, R′ = nBu, 1e; R = p‐MeOC6H4, R′ = Me, 1f; Tol = 4‐C6H4Me, Xyl = 2,6‐Me2C6H3) with CS2 and NaH resulted in the replacement of CH hydrogen (in the bridging frame) with CS2 to give the corresponding dithiocarboxylate‐vinyliminium complexes [Fe2{μ‐η1:η3‐C(R′)=C(CS2)C=N(Me)(R)}(μ‐CO)(CO)(Cp)2] (2a–2f). Analogously, 1a and 1d reacted with NaH and SCNPh to afford the complexes[Fe2{μ‐η1:η3‐C(R′)=C{C(NPh)S}C=N(Me)(R)}(μ‐CO)(CO)(Cp)2](R = Xyl, R′ = Me, 3a; R = Me, R′ = Me, 3b), respectively. Complex 2b was methylated at the dithiocarboxylate group upon treatment with CH3SO3CF3 to yield [Fe2{μ‐η1:η3‐C(R′)=C(CS2Me)C=N(Me)(Xyl)}(μ‐CO)(CO)(Cp)2][SO3CF3] (4a). Likewise, the zwitterionic complexes 2d–f underwent addition of the metal fragment [Fe(CO)2Cp]+ at the dithiocarboxylate group to yield the corresponding triiron complexes [Fe2{μ‐η1:η3‐C(R′)=C(CS2Fp)C=N(Me)(R)}(μ‐CO)(CO)(Cp)2][SO3CF3] [R = R′ = Me, 4b; R = Me, R′ = nBu, 4c; R = p‐MeOC6H4, R′ = Me, 4d; Fp = Fe(CO)2Cp]. In a related reaction with [Pd(CH3CN)2Cl2], 2a binded to Pd through the dithiocarboxylate group as chelating ligand to afford the complex [PdCl2{κ2‐(S,S)‐2a}] (5). The X‐ray structures of 2b, 3a and 4d have been determined.
Studies on Tetrahydrofuran‐Based Highly O‐Functionalized Alkynes: Applications to Synthesis of Tetrahydrofuranyl‐Polyynes and C‐Nucleoside Analogues
Authors: Reddy P. Venkat , Bajpai Vikas , Kumar Brijesh , Shaw Arun K. 
Published: January 2011

AbstractThe enantiomerically pure tetrahydrofuranylalkynes 17–20 were synthesized by using various methods. By utilizing these alkynes, an efficient CuI‐catalysed sp‐sp carbon homo‐coupling protocol in dry DMF without other additives (like amine or base, phosphane and palladium catalyst) has been developed. The method has been applied to the synthesis of symmetrical butadiynyl (32–37), octatetraynyl (47) and dodecahexaynyl (49) polyynes. The unsymmetrically substituted diynes 39, 40 and 41 and triynes 43 and 44 were synthesized by coupling reaction between the newly synthesized bromoalkyne/diynes 38/42 and commercially available (trialkylsilyl)acetylenes. Syntheses of the 1,2,3‐triazolyl‐C‐nucleoside analogue 51 and of the ethynyl‐ and butadiynyl‐bridged 1,2,3‐triazolyl‐C‐nucleoside analogues 52 and 53 were performed by 1,3‐dipolar cycloaddition reaction (“click“ chemistry) of their respective alkyne precursors.
Synthesis of Sulfones by Iron‐Catalyzed Decomposition of Sulfonylhydrazones
Authors: Barluenga José , Tomás‐Gamasa María , Aznar Fernando , Valdés Carlos 
Published: January 2011

AbstractThe Fe‐catalyzed decomposition of sulfonylhydrazones gives rise to sulfones. The reaction is quite general and allows the preparation of sulfones from a variety of aryl, alkyl, and α,β‐unsaturated aldehydes and ketones. Crossover experiments reveal that the reaction is an intermolecular process, which may proceed by nucleophilic attack of the sulfinate anion on an iron carbene complex.
Divergent Approach to Gabosines and Anhydrogabosines: Enantioselective Syntheses of (+)‐Epiepoformin, (+)‐EpoKformin, (+)‐Gabosine A, and Gabosines B and F
Authors: Toribio Gladis , Marjanet Georgina , Alibés Ramon , de March Pedro , Font Josep , Bayón Pau , Figueredo Marta 
Published: January 2011

AbstractA divergent approach to polyoxygenated methylcyclohexanes has been applied to synthesize several gabosines and anhydrogabosines. The starting hydroxycyclohexenone, which is readily available in any antipodal form, provides access to both enantiomers of the target compounds. The syntheses of anhydrogabosines involve three main transformations: α‐methylation, epoxidation, and sulfur removal, whereas the syntheses of gabosines require an additional epoxide hydrolysis step. The strategy has been applied to the synthesis of (+)‐epiepoformin, (+)‐epoformin, (+)‐gabosine A, and gabosines B and F, through straightforward sequences in good overall yields.
Easy Access to Pyranoacridines, Pyranoxanthenes, and Arylchromenes Through a Domino Reaction
Authors: Commandeur Claude , Florent Jean‐Claude , Rousselle Patricia , Bertounesque Emmanuel 
Published: January 2011

AbstractA series of pyrano[2,3,4‐kl]acridines has been synthesized through an unprecedented domino reaction involving1‐hydroxy‐3‐methoxy‐10‐methylacridone and Grignard reagents. This method has been successfully applied to xanthone, anthracenone, and benzophenone derivatives, thus providing novel access to pyranoxanthenes, dihydronaphthochromenes, and arylchromenes.
ZNF703 is a common Luminal B breast cancer oncogene that differentially regulates luminal and basal progenitors in human mammary epithelium
Authors: Holland Daniel G. , Burleigh Angela , Git Anna , Goldgraben Mae A. , Perez‐Mancera Pedro A. , Chin Suet‐Feung , Hurtado Antonio , Bruna Alejandra , Ali H. Raza , Greenwood Wendy , Dunning Mark J. , Samarajiwa Shamith , Menon Suraj , Rueda Oscar M. , Lynch Andy G. , McKinney Steven , Ellis Ian O. , Eaves Connie J. , Carroll Jason S. , Curtis Christina , Aparicio Samuel , Caldas Carlos 
Published: January 2011

AbstractThe telomeric amplicon at 8p12 is common in oestrogen receptor‐positive (ER+) breast cancers. Array‐CGH and expression analyses of 1172 primary breast tumours revealed that ZNF703 was the single gene within the minimal amplicon and was amplified predominantly in the Luminal B subtype. Amplification was shown to correlate with increased gene and protein expression and was associated with a distinct expression signature and poor clinical outcome. ZNF703 transformed NIH 3T3 fibroblasts, behaving as a classical oncogene, and regulated proliferation in human luminal breast cancer cell lines and immortalized human mammary epithelial cells. Manipulation of ZNF703 expression in the luminal MCF7 cell line modified the effects of TGFβ on proliferation. Overexpression of ZNF703 in normal human breast epithelial cells enhanced the frequency of in vitro colony‐forming cells from luminal progenitors. Taken together, these data strongly point to ZNF703 as a novel oncogene in Luminal B breast cancer.
Hysteretic behavior and strength capacity of shallowly embedded steel column bases with SFRCC slab
Authors: Cui Yao , Nakashima Masayoshi 
Published: January 2011

AbstractA series of shallowly embedded steel column bases consisting of an exposed column base and a floor slab is tested under horizontal cyclic loading to very large deformation. The effects of floor slabs on strength and ductility are examined using concrete and Steel fiber‐reinforced cementitous composites (SFRCC) for the floor slab. The elastic stiffness, maximum strength, and dissipated energy of the column bases when they include SFRCC increase by 40, 70, and 70% over those of corresponding column bases with concrete floor slab. Better bonding behavior is notable for SFRCC, and the maximum strength and dissipated energy further increase by 15–30% and 70–90%, respectively, owing to the careful arrangement of reinforcing bars. Numerical models are developed to enhance the understanding the behavior of shallowly embedded column bases. Procedures for estimating the elastic stiffness and maximum strength of shallowly embedded column bases with conventional concrete are calibrated for their applicability to those with SFRCC slab. Copyright © 2011 John Wiley & Sons, Ltd.
Understanding your own and other's minds: The relationship to eating disorder related symptoms
Authors: Warren Louise , Cooper Myra J. 
Published: January 2011

AbstractA study using a cross‐sectional survey design examined whether eating disorder (ED) related symptoms are associated with understanding one's own and others' minds. A non‐clinical sample of 145 women completed self‐report questionnaires and recorded their emotional, cognitive and behavioural responses to descriptions of scenarios (vignettes). Responses to scenarios were made from the perspective of self, an attachment figure and a same sex acquaintance. Data were analysed using multiple regression statistics with ED related symptoms as the dependent variable. High levels of ED related symptoms were associated with ‘concretised’ understanding of own emotions (i.e. a greater number of food related responses), but sophisticated understanding of same sex acquaintance's emotions. They were associated with fewer positive thoughts for self, fewer negative emotions about their own behaviour, and more food responses for same sex acquaintance's behaviour. Similarities and differences were observed in the different perspectives. Limitations are discussed. Implications for further research related to this topic and relevant to EDs are briefly summarised. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Space‐Earth absorption in the absence of rain: Radiometric inversion coefficients for Italy
Authors: Barbaliscia Francesco , Guerriero Leila , Schiavon Giovanni , Solimini Domenico 
Published: January 2011

AbstractSpace‐earth atmospheric attenuation in absence of rain can be estimated from atmospheric physical parameters, which are, in turn, obtainable from radiometric measurements of the sky brightness temperature. In this paper the radiometric inversion coefficients needed to retrieve the total vapour and liquid water content of the atmosphere from radiometric measurements are computed making use of a statistically significant data‐ base of radiosonde vertical profiles. Linear regression is applied to data collected over ten years at five Italian sites to derive the inversion coefficients under various operational hypotheses. Site‐to‐site and seasonal variabilities are analysed as well as the influence of both the instrumental characteristics and the criterium for detecting cloud liquid. A first set of values valid for the Italian territory is presented for system design purpose.
Digital video and speech transmission in existing land mobile radio networks
Authors: Pelz R. Mann , Benthin M. , Kammeyer K. ‐D. 
Published: January 2011

AbstractThe development of very low bit rate image compression algorithms renders the transmission of moving and still video with associated speech in a variety of existing and future narrowband telecommunication networks. Existing analog, narrowband (mobile) networks represent a potential field for the introduction of digital bearer services under utilization of the underlying system resources in a compatible manner. A broader variety of services in coexistence with existing analog services may be provided without new network planning. This paper presents corresponding techniques for video and speech transmission in the existing Nonpublic Land Mobile Network under application of commercially available radio telephones and an additional, external digital signal processing unit. Special attention is given to the choice of a digital modulation method under consideration of network constraints such as adjacent channel interference and linear distortions due to nonideal analog IF filters. In this context a modified maximum likelihood detector based on an equivalent linear model of the baseband transmission link is presented. For robust low bit rate video transmission a combined source‐channel coding scheme with unequal error protection matched to the source and channel characteristics in case of the target mobile narrowband network is proposed.
Code division multiple access schemes for terrestrial and satellite mobile communication networks: Modeling and performance evaluation
Authors: Del Re Enrico , Fantacci Romano , Pazzaglia Antonio 
Published: January 2011

AbstractThis paper deals with the modeling and performance evaluation of different Code Division Multiple Access schemes suitable for applications in terrestrial and satellite mobile communication networks. The use of particular propagation channel models and the relative sensitivity of different demodulation schemes to their parameters are the subject the paper is focused on. In particular, an extension to the case of wideband communications of Lutz's satellite channel model is presented. The main goal is to analyze how the increased interference level, typical of a multipath environment, can influence different demodulator architectures in different propagation conditions. Perfectly coherent and differential modulation methods are employed with conventional and RAKE receivers to derive the error rate performance by computer simulations. Some issues concerning the characterization of the propagation channel models in the case of the RAKE receiver are also investigated and discussed.