bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel microRNA mmu‐miR‐466h affects apoptosis regulation in mammalian cells
Authors: Druz Aliaksandr , Chu Chia , Majors Brian , Santuary Rodell , Betenbaugh Michael , Shiloach Joseph 
Published: February 2011

AbstractThis study determined the changes in microRNA (miRs) expression in mammalian Chinese hamster ovary (CHO) cells undergoing apoptosis induced by exposing the cells to nutrient‐depleted media. The apoptosis onset was confirmed by reduced cell viability and Caspase‐3/7 activation. Microarray comparison of known mouse and rat miRs in CHO cells exposed to fresh or depleted media revealed up‐regulation of the mouse miR‐297‐669 cluster in CHO cells subjected to depleted media. The mmu‐miR‐466h was chosen for further analysis as the member of this cluster with the highest overexpression and its up‐regulation in depleted media was confirmed with qRT‐PCR. Since miRs suppress mRNA translation, we hypothesized that up‐regulated mmu‐miR‐466h inhibits anti‐apoptotic genes and induces apoptosis. A combination of bioinformatics and experimental tools was used to predict and verify mmu‐miR‐466h anti‐apoptotic targets. 8708 predicted targets were obtained from miRecords database and narrowed to 38 anti‐apoptotic genes with DAVID NCBI annotation tool. Several genes were selected from this anti‐apoptotic subset based on nucleotide pairing complimentarity between the mmu‐miR‐466h seed region and 3′ UTR of the target mRNAs. The qRT‐PCR analysis revealed reduced mRNA levels of bcl2l2, dad1, birc6, stat5a, and smo genes in CHO cells exposed to depleted media. The inhibition of the mmu‐miR‐466h increased the expression levels of those genes and resulted in increased cell viability and decreased Caspase‐3/7 activation. The up‐regulation of mmu‐miR‐466h in response to nutrients depletion causes the inhibition of several anti‐apoptotic genes in unison. This suggests the pro‐apoptotic role of mmu‐miR‐466h and its capability to modulate the apoptotic pathway in mammalian cells. Biotechnol. Bioeng. 2011; 108:1651–1661. © 2011 Wiley Periodicals, Inc.
Glucose oxidase‐mediated polymerization as a platform for dual‐mode signal amplification and biodetection
Authors: Berron Brad J. , Johnson Leah M. , Ba Xiao , McCall Joshua D. , Alvey Nicholas J. , Anseth Kristi S. , Bowman Christopher N. 
Published: February 2011

AbstractWe report the first use of a polymerization‐based ELISA substrate solution employing enzymatically mediated radical polymerization as a dual‐mode amplification strategy. Enzymes are selectively coupled to surfaces to generate radicals that subsequently lead to polymerization‐based amplification (PBA) and biodetection. Sensitivity and amplification of the polymerization‐based detection system were optimized in a microwell strip format using a biotinylated microwell surface with a glucose oxidase (GOx)–avidin conjugate. The immobilized GOx is used to initiate polymerization, enabling the detection of the biorecognition event visually or through the use of a plate reader. Assay response is compared to that of an enzymatic substrate utilizing nitroblue tetrazolium in a simplified assay using biotinylated wells. The polymerization substrate exhibits equivalent sensitivity (2 µg/mL of GOx‐avidin) and over three times greater signal amplification than this traditional enzymatic substrate since each radical that is enzymatically generated leads to a large number of polymerization events. Enzyme‐mediated polymerization proceeds in an ambient atmosphere without the need for external energy sources, which is an improvement upon previous PBA platforms. Substrate formulations are highly sensitive to both glucose and iron concentrations at the lowest enzyme concentrations. Increases in amplification time correspond to higher assay sensitivities with no increase in non‐specific signal. Finally, the polymerization substrate generated a signal to noise ratio of 14 at the detection limit (156 ng/mL) in an assay of transforming growth factor‐beta. Biotechnol. Bioeng. 2011; 108:1521–1528. © 2011 Wiley Periodicals, Inc.
Sequential assembly of cell‐laden hydrogel constructs to engineer vascular‐like microchannels
Authors: Du Yanan , Ghodousi Majid , Qi Hao , Haas Nikhil , Xiao Wenqian , Khademhosseini Ali 
Published: February 2011

AbstractMicroscale technologies, such as microfluidic systems, provide powerful tools for building biomimetic vascular‐like structures for tissue engineering or in vitro tissue models. Recently, modular approaches have emerged as attractive approaches in tissue engineering to achieve precisely controlled architectures by using microengineered components. Here, we sequentially assembled microengineered hydrogels (microgels) into hydrogel constructs with an embedded network of microchannels. Arrays of microgels with predefined internal microchannels were fabricated by photolithography and assembled into 3D tubular construct with multi‐level interconnected lumens. In the current setting, the sequential assembly of microgels occurred in a biphasic reactor and was initiated by swiping a needle to generate physical forces and fluidic shear. We optimized the conditions for assembly and successfully perfused fluids through the interconnected constructs. The sequential assembly process does not significantly influence cell viability within the microgels indicating its promise as a biofabrication method. Finally, in an attempt to build a biomimetic 3D vasculature, we incorporated endothelial cells and smooth muscle cells into an assembled construct with a concentric microgel design. The sequential assembly is simple, rapid, cost‐effective, and could be used for fabricating tissue constructs with biomimetic vasculature and other complex architectures. Biotechnol. Bioeng. 2011; 108:1693–1703. © 2011 Wiley Periodicals, Inc.
Design and construction of improved new vectors for Zymomonas mobilis recombinants
Authors: Dong Hong‐Wei , Bao Jie , Ryu Dewey D.Y. , Zhong Jian‐Jiang 
Published: February 2011

AbstractZymomonas mobilis is a very important gram‐negative bacterium having a potential application to simultaneous co‐production of biofuel and other high value‐added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its application has been limited due to its relatively low transformation efficiency, a large plasmid size (13.4 kb), and limited choice of cloning sites for gene manipulations. Some of these limitations can be overcome by the newly designed and constructed plasmid pHW20a, which provides significantly higher transformation efficiency (about two orders of magnitude greater), better stability (for at least 120 generation times), and an ease of gene manipulations. The pHW20a contains three complete cis‐acting genes (repA, repB, and repC) encoding the Rep proteins for primosome formation. It has the origin of replication (oriV) to ensure replication in gram‐negative bacteria, two mob genes that enhances transformation efficiency, a screening marker (lacZα), expanded multiple cloning sites (MCS) that enables easy gene manipulation, and the tetracycline resistance gene (tcr). The utility of screening marker, lacZα with MCS, was confirmed by the blue‐white screening test. Several examples of applications of gene expression in Z. mobilis ZM4 have been demonstrated in this article by using several new pHW20a‐derived plasmids and expressing the homologous genes (gfo and ppc) and the heterologous genes (bglA, mdh, and fdh1). The results show that pHW20a is a very useful new vector for construction of new Z. mobilis recombinant strains that will enable simultaneous co‐production of biofuel and high value added products. Biotechnol. Bioeng. 2011; 108:1616–1627. © 2011 Wiley Periodicals, Inc.
Room temperature ionic liquids as emerging solvents for the pretreatment of lignocellulosic biomass
Authors: Mora‐Pale Mauricio , Meli Luciana , Doherty Thomas V. , Linhardt Robert J. , Dordick Jonathan S. 
Published: February 2011

AbstractRoom temperature ionic liquids (RTILs) are emerging as attractive and green solvents for lignocellulosic biomass pretreatment. The unique solvating properties of RTILs foster the disruption of the 3D network structure of lignin, cellulose, and hemicellulose, which allows high yields of fermentable sugars to be produced in subsequent enzymatic hydrolysis. In the current review, we summarize the physicochemical properties of RTILs that make them effective solvents for lignocellulose pretreatment including mechanisms of interaction between lignocellulosic biomass subcomponents and RTILs. We also highlight several recent strategies that exploit RTILs and generate high yields of fermentable sugars suitable for downstream biofuel production, and address new opportunities for use of lignocellulosic components, including lignin. Finally, we address some of the challenges that remain before large‐scale use of RTILs may be achieved. Biotechnol. Bioeng. 2011;108:1229–1245. © 2011 Wiley Periodicals, Inc.
FPGA‐Matlab‐based open core for three‐time controllers in automatic control applications
Authors: de Jesus Rangel‐Magdaleno Jose , Rivera‐Guillen Jesus Rooney , de Jesus Romero‐Troncoso Rene , Osornio‐Rios Roque Alfredo 
Published: February 2011

AbstractAutomatic control is present in most industrial applications and engineering. The automatic control practice with the physical implementation of the controller is a major issue to be covered in undergraduate/graduate engineering course, and those issues dealing with subjects such as automation, control, electromechanics, mechatronics, manufacturing, and robotics which should be included in their curricula. The contribution of this work is the development of an open core of a three‐time controller under FPGA technology applied in a standard control course, allowing students to have a real‐time control implementation with technological independence and high level of integration. The combination of both, theory and practice is important. Thus, this course's proposal develops the theoretical bases in practical form. The opportunity to control a servomotor demands students with area proficiency, which is reflected in a project development. © 2011 Wiley Periodicals, Inc. Comput Appl Eng Educ 21:E132–E140, 2013
A quantum chemical and chemometric study of sesquiterpene lactones with cytotoxicity against tumor cells
Authors: Arantes Francisco F. P. , Barbosa Luiz C. A. , Maltha Célia R. A. , Demuner Antônio J. , Fidêncio Paulo H. , Carneiro José Walkimar M. 
Published: February 2011

AbstractThe semi‐empirical molecular orbital method PM6 was employed to calculate a set of molecular descriptors of 20 sesquiterpene lactones (SQLs) with cytotoxicity against HL‐60 (leukemia) tumor cells. The principal component analysis (PCA) and hierarchical cluster analysis (HCA) methods were used to obtain possible relationships between the calculated descriptors and the biological activity of the lactones. Four descriptors were identified as responsible for the separation between the active and inactive compounds: EHOMO (highest occupied molecular orbital energy); Q11 (net atomic charge on C11); Q12 (net atomic charge on C12) and Q13 (net atomic charge on C13). These results indicated that the presence of the α‐methylene‐γ‐lactone group has a significant role in the mechanism by which SQLs exert their biological activities.In order to deepen our knowledge about the structure–activity relationship of sesquiterpene lactones (SQLs), a quantum chemical and chemometric study of sesquiterpene lactones with cytotoxicity was performed against HL‐60 (leukemia) tumor cells. The analysis of the molecular descriptors identified as responsible for the separation between the active and inactive compounds indicated that the presence of the α‐methylene‐γ‐lactone functionality has a significant role in the mechanism by which SQLs exert their biological activities. Copyright © 2011 John Wiley & Sons, Ltd.
ChemInform Abstract: Synthesis of New LnxBi2‐xSe3 (Ln: Sm3+, Eu3+, Gd3+, Tb3+) Nanomaterials and Investigation of Their Optical Properties.
Authors: Alemi Abdolali , Klein Axel , Meyer Gerd , Dolatyari Mahboubeh , Babalou Aliakbar 
Published: February 2011

AbstractThe new luminescent title materials (0 ≤ x ≤ 0.6) are synthesized by hydrothermal reaction of Bi(NO3)3, Sm2O3, Eu2O3, Gd2O3, Tb4O7, grey selenium, EDTA, NaOH, N2H4·H2O, and H2O (autoclave, 180 °C, 48 h, 90—95% yield).
ChemInform Abstract: Structural and Magnetic Properties of C15 HoMn2 Hydrides.
Authors: Budziak A. , Zachariasz P. , Kolwicz‐Chodak L. , Figiel H. , Pacyna A. , Zukrowski J. 
Published: February 2011

AbstractPowder samples of cubic HoMn2Hx (0 ≤ x ≤ 4.3) are characterized by powder XRD and AC/DC magnetometry as a function of temperature and external magnetic field.
ChemInform Abstract: Synthesis, Crystal Structure and Nonlinear Optical Property of Rb3V5O14.
Authors: Pan Jianguo , Li Yuebao , Cui Yuejie , Zhao Lingyan , Li Xing , Han Lei 
Published: February 2011

AbstractThe title compound is synthesized by solid state reaction of Rb2CO3 with 3% excess V2O5 (500 °C, Pt crucible, 24 h).
ChemInform Abstract: Crystal Structure, Thermal and Magnetic Properties of Cr2V4O13.
Authors: Patwe S. J. , Achary S. N. , Manjanna J. , Kadam R. M. , Salunke H. G. , Tyagi A. K. 
Published: February 2011

AbstractThe title compound is synthesized by solid state reaction of stoichiometric amounts of V2O5 and Cr2O3 at 630 °C and its crystal structure is determined by powder XRD.
ChemInform Abstract: Chiral Primary Amine Thiourea Promoted Highly Enantioselective Michael Reactions of Isobutyraldehyde with Maleimides.
Authors: Bai Jian‐Fei , Peng Lin , Wang Liang‐liang , Wang Li‐Xin , Xu Xiao‐Ying 
Published: February 2011

AbstractA variety of N‐aryl and N‐alkyl maleimides react smoothly with isobutylaldehyde in the presence of a chiral diamine‐derived thiourea catalyst to give the Michael adducts in excellent yields and enantioselectivities (up to 99% e.e.).
ChemInform Abstract: Aqueous Aza‐Michael Reaction of Conjugated Alkenes: Toward Spermine.
Authors: Joshi Jigar H. , Saiyed Akeel S. , Bedekar Ashutosh V. 
Published: February 2011

AbstractAza‐Michael reaction of amines with conjugated alkenes in water is efficiently achieved without the addition of any additives.
ChemInform Abstract: Highly Chemoselective Palladium‐Catalyzed Cross‐Trimerization Between Alkyne and Alkenes Leading to 1,3,5‐Trienes or 1,2,4,5‐Tetrasubstituted Benzenes with Dioxygen.
Authors: Zhou Peng , Huang Liangbin , Jiang Huanfeng , Wang Azhong , Li Xianwei 
Published: February 2011

AbstractThe O2/LiCl/K2CO3‐mediated reaction of alkynes with an excess of alkenes allows new and environmentally friendly access to 1,3,5‐trienes.
ChemInform Abstract: InCl3/CyNH2 Cocatalyzed Carbocyclization Reaction: An Entry to α‐Disubstituted exo‐Methylene Cyclopentanes
Authors: Montaignac Benjamin , Vitale Maxime R. , Ratovelomanana‐Vidal Virginie , Michelet Veronique 
Published: February 2011

Abstractvia an efficient and cheap access
ChemInform Abstract: Cinchona Alkaloid‐Catalyzed Asymmetric Trifluoromethylation of Alkynyl Ketones with Trimethylsilyl Trifluoromethane.
Authors: Kawai Hiroyuki , Tachi Kentaro , Tokunaga Etsuko , Shiro Motoo , Shibata Norio 
Published: February 2011

AbstractProduct (Va) is a suitable substrate for the synthesis of biologically attractive aryl and heteroaryl trifluoromethyl carbinols.
ChemInform Abstract: Reusable Polystyrene‐Supported Pd Catalyst for Mizoroki—Heck Reactions with Extremely Low Amounts of Supported Pd.
Authors: Diebold Carine , Schweizer Stephane , Becht Jean‐Michel , Le Drian Claude 
Published: February 2011

AbstractThe catalyst can be reused three times with significant yield drop afterwards.
ChemInform Abstract: 1,3,2,4‐Diazadiphosphetidines as Ligand and Base for Palladium‐Catalyzed Suzuki‐Miyaura, Sonogashira—Hagihara, and Homocoupling Reactions of Aryl Halides under Heterogeneous Conditions in Water.
Authors: Iranpoor Nasser , Firouzabadi Habib , Tarassoli Abbas , Fereidoonnezhad Masood 
Published: February 2011

AbstractA novel diazadiphosphetidine is prepared and successfully applied as a ligand in a variety of palladium‐catalyzed coupling reactions of halides (I) in water.
ChemInform Abstract: Polyethylene Glycol (PEG 300) and Water—Ethanol as Benign Solvent Systems for the Synthesis of a Novel Series of 2‐Hydroxynaphthalen‐1(4H)‐ones.
Authors: Villemin D. , Benabdallah M. , Choukchou‐Braham N. , Mostefa‐Kara B. 
Published: February 2011

AbstractIn water‐ethanol at 40 °C the yields are lower than using the presented grinding method.
ChemInform Abstract: Synthesis of Substituted (S)‐2‐Aminotetralins via Ring‐Opening of Aziridines Prepared from L‐Aspartic Acid β‐tert‐Butyl Ester.
Authors: Aaseng Jon Erik , Gautun Odd R. 
Published: February 2011

AbstractKey steps of the synthesis of optically active aminotetralins (VI) are the regioselective ring opening of aziridine (II) (or its Cbz analogue) with in situ generated arylcuprate reagents as well as Friedel—Crafts cyclization of the free acids (IV) (not optimized).
ChemInform Abstract: Synthesis of β‐Trifluoromethylated Δ1‐Pyrrolines.
Authors: Marrec Olivier , Christophe Carole , Billard Thierry , Langlois Bernard , Vors Jean‐Pierre , Pazenok Sergii 
Published: February 2011

AbstractTrifluoromethylated title compounds (III) are easily synthesized in a one‐pot procedure from β‐(trifluoromethyl)enones and isolated predominantly as the trans‐isomer.
ChemInform Abstract: Facile Synthesis of 2‐Bromoindoles by Ligand‐Free CuI‐Catalyzed Intramolecular Cross‐Coupling of gem‐Dibromoolefins.
Authors: Jiang Baishan , Tao Kemei , Shen Wang , Zhang Jiancun 
Published: February 2011

AbstractOptimal results are obtained if mesyl‐substituted aniline substrates are used.
ChemInform Abstract: Synthesis of Fluorine‐Containing Benzimidazole Derivatives.
Authors: Vasilyeva T. P. , Karimova N. M. , Slavich Yu. V. 
Published: February 2011

AbstractConvenient methods for the synthesis of benzimidazoles containing fluoroalkyl substituents in position 2 starting from phenylenediamines (I) are developed.
ChemInform Abstract: C—H Bond Functionalization in the Synthesis of Fused 1,2,3‐Triazoles.
Authors: Panteleev Jane , Geyer Karolin , Aguilar‐Aguilar Angelica , Wang Letian , Lautens Mark 
Published: February 2011

AbstractA synthesis of polycyclic frameworks including a 1,2,3‐triazole unit is developed.
ChemInform Abstract: An Easy Synthetic Approach to 1,2,3‐Triazole‐Fused Heterocycles.
Authors: Fiandanese Vito , Marchese Giuseppe , Punzi Angela , Iannone Francesco , Rafaschieri Giacomo G. 
Published: February 2011

AbstractAn efficient synthesis of triazole‐fused isoindoles and isoquinolines is presented.
ChemInform Abstract: Reaction of Heterocyclic Enamines with Nitrile Oxide and Nitrilimine Precursors.
Authors: Altug Cevher , Dueruest Yasar , Elliot Mark C. , Kariuki Benson M. , Rorstad Tillique , Zaal Mark 
Published: February 2011

AbstractA range of novel heterocyclic compounds is prepared from alkylidenepyrrolidines and 1,3‐dipoles such as nitrile oxides, nitrilimines or their precursors.
ChemInform Abstract: Organocatalytic Asymmetric Sulfa‐Michael/Michael Addition Reactions: A Strategy for the Synthesis of Highly Substituted Chromans with a Quaternary Stereocenter.
Authors: Wang Xu‐Fan , Hua Qiu‐Lin , Cheng Ying , An Xiao‐Lei , Yang Qing‐Qing , Chen Jia‐Rong , Xiao Wen‐Jing 
Published: February 2011

AbstractThe title process provides an efficient access to various chroman derivatives with good to excellent diastereo‐ and enantioselectivity and can be extended to cyclohexane analogues [cf.
ChemInform Abstract: Rh(III)‐Catalyzed Oxidative Coupling of N‐Aryl‐2‐aminopyridine with Alkynes and Alkenes.
Authors: Chen Jinlei , Song Guoyong , Pan Cheng‐Ling , Li Xingwei 
Published: February 2011

AbstractThe aminopyridine substrates undergo oxidative coupling with alkynes and acrylates under conditions A) to generate N‐pyridyl‐substituted indoles or N‐(2‐pyridyl)‐quinolones, respectively.
ChemInform Abstract: Synthesis and Antibacterial Properties of Some Novel 2‐Substituted‐6‐(4‐methyl‐6‐substitutedcinnoline‐3‐yl)imidazo[2,1‐b] [1,3,4]thiadiazoles.
Authors: Jakhar Anshu , Makrandi J. K. 
Published: February 2011

AbstractThe title compounds (V) show strong activity against Gram‐negative bacteria (E.
ChemInform Abstract: Synthesis and Cytotoxic Activity of Heterocyclization Products of 1,1‐Dicyano‐2‐hetaryl‐2‐trifluoromethylethylenes.
Authors: Pavlova Yu. E. , Shidlovskii A. F. , Gusev D. B. , Peregudov A. S. , Bulychev Yu. N. , Chkanikov N. D. 
Published: February 2011

AbstractA variety of novel Fluorinated N‐heterocycles is synthesized by heterocyclization reactions of the title olefins (II) with nitrogen compounds.
ChemInform Abstract: Synthesis of 3‐(Benzimidazol‐2‐yl‐amino)‐1‐ (4‐bromophenyl)‐2‐thioxopyrimidin‐4,6‐dione Analogues and Their Antimicrobial Activity.
Authors: Goudgaon N. M. , Basha N. Jeelan 
Published: February 2011

AbstractAll synthesized compounds are tested for antibacterial (P.
ChemInform Abstract: Microwave Assisted Synthesis of Some New 2‐Substituted‐1,4‐benzothiazine Derivatives.
Authors: Deshmukh M. B. , Salunkhe S. M. , Anbhule P. V. , Patil D. R. 
Published: February 2011

AbstractA simple and eco‐friendly synthesis is developed by using inexpensive reagents.
ChemInform Abstract: Substituted 1‐Nitro‐2‐phenylethenes in Reaction with N‐Phenacyl‐ and N‐Acetonylisoquinolinium Bromides.
Authors: Nosachev S. B. , Del'netskaya K. A. , Solov'ev N. A. , Tyrkov A. G. 
Published: February 2011

AbstractThe reaction of isoquinolinium bromides (I) with dipolarophiles (II) gives rise to cycloadducts (III), which are readily converted into novel triazinoindolizine derivatives (IV).
ChemInform Abstract: Cyclothiomethylation of Carboxylic Acid Hydrazides with Aldehydes and H2S.
Authors: Akhmetova V. R. , Khairullina R. R. , Tyumkina T. V. , Nelyubina Yu. V. , Smol'yakov A. F. , Bushmarinov I. S. , Starikova Z. A. , Abdullin M. F. , Kunakova R. V. 
Published: February 2011

AbstractThe cyclothiomethylation of carboxylic acid hydrazides (I) with formaldehyde and H2S affords thiadiazoline (III) and dithiazinane derivatives (IV) in ratios depending on the reaction conditions.
ChemInform Abstract: Simple and Efficient Synthesis of Fmoc/Boc/Cbz‐Protected‐β‐amino Alcohols and Peptidyl Alcohols Employing Boc2O.
Authors: Lalithamba H. S. , Sureshbabu Vommina V. 
Published: February 2011

AbstractA procedure is given for the reduction of the carboxyl group of N‐protected peptides and dipeptides to the corresponding alcohols.