bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Frequency of Skin Lesions in Guilan Province, Iran
Authors: Alizadeh Narges , Darjani Abbas , Rafiei Rana , Gharaei Nejad Kaveh , Khoshhal Ziabari Amirhosein , Eftekhari Hojat 
Published: May 2024

Introduction
Skin tumors as one of the patient's most concerning health issues have a varied spectrum, some of which can be fatal. In this study, we aimed to evaluate the frequency of various skin lesions and the associated risk factors among patients.

Methods
This cross-sectional study was conducted from 2016 to 2021 on 559 skin lesions diagnosed by dermatology specialists. Demographical and clinical data and various skin lesions (skin malformations, benign, premalignant, and malignant tumors) were recorded and analyzed using IBM SPSS Statistics Version 21 based on a significance level < .05.

Results
The findings indicated an age-dependent increase in the prevalence of skin lesions. Females had a higher frequency of benign tumors, whereas males represented a higher rate of malignant tumors. About 53%, 6.8%, and 40.1% of skin lesions were benign, premalignant, and malignant tumors, respectively. Among detected tumors, seborrheic keratosis, actinic keratosis, and basal cell carcinoma were the most prevalent as benign, premalignant, and malignant tumors, respectively. The high level of diagnostic accuracy among clinical and histopathological findings illustrated a significant agreement (p < .05).

Conclusion
Our findings showed that malignant skin tumors were more prevalent in males and in patients with an upper age, which suggests more consideration for better preventive policies in these populations.
Impact of Collaborative Leadership, Workplace Social Capital, and Interprofessional Collaboration Practice on Patient Safety Climate
Authors: Kida Ryohei , Fujitani Katsumi , Matsushita Hironobu 
Published: May 2024

ABSTRACT

Purpose:
Patient safety climate is an important factor in promoting patient safety for healthcare organizations. This study investigated the relationship between collaborative leadership and patient safety climate, the mediation effect of workplace social capital, or interprofessional collaboration practice.

Methods:
A web-based cross-sectional questionnaire survey was administered between May 2021 and May 2022, to employees of three acute care hospitals in Japan. The relationship between variables was verified by structural equation modeling.

Results:
A total of 1,276 staff members participated in the study. Collaborative leadership affected the workplace social capital (β = .734) and interprofessional collaboration (β = .561), which were positively associated with patient safety climate (β = .403 and .405, respectively), verifying the mediating relationship of workplace social capital and interprofessional collaboration between collaborative leadership and patient safety climate.

Conclusions:
Collaborative leadership enhances the reciprocity and interprofessional practices of the healthcare team. The interaction among interprofessional team members fosters a patient safety climate. The results of this survey suggest that the development of collaborative leadership, which encourages interprofessional collaboration and fosters workplace social capital, is inherently crucial for cultivating a patient safety climate.
Single-cell and bulk RNA-sequence identiﬁed ﬁbroblasts signature and CD8 + T-cell - ﬁbroblast subtype predicting prognosis and immune therapeutic response of bladder cancer, based on machine learning: bioinformatics multi-omics study
Authors: Li Jingxian , Kong Zheng , Qi Yuanjiong , Wang Wei , Su Qiang , Huang Wei , Zhang Zhihong , Li Shuai , Du E 
Published: May 2024

Background:
Cancer-associated fibroblasts (CAFs) are found in primary and advanced tumours. They are primarily involved in tumour progression through complex mechanisms with other types of cells in the tumour microenvironment. However, essential fibroblasts-related genes (FRG) in bladder cancer still need to be explored, and there is a shortage of an ideal predictive model or molecular subtype for the progression and immune therapeutic assessment for bladder cancer, especially muscular-invasive bladder cancer based on the FRG.

Materials and methods:
CAF-related genes of bladder cancer were identified by analysing single-cell RNA sequence datasets, and bulk transcriptome datasets and gene signatures were used to characterize them. Then, 10 types of machine learning algorithms were utilised to determine the hallmark FRG and construct the FRG index (FRGI) and subtypes. Further molecular subtypes combined with CD8+ T-cells were established to predict the prognosis and immune therapy response.

Results:
Fifty-four BLCA-related FRG were screened by large-scale scRNA-sequence datasets. The machine learning algorithm established a 3-genes FRGI. High FRGI represented a worse outcome. Then, FRGI combined clinical variables to construct a nomogram, which shows high predictive performance for the prognosis of bladder cancer. Furthermore, the BLCA datasets were separated into two subtypes – fibroblast hot and cold types. In five independent BLCA cohorts, the fibroblast hot type showed worse outcomes than the cold type. Multiple cancer-related hallmark pathways are distinctively enriched in these two types. In addition, high FRGI or fibroblast hot type shows a worse immune therapeutic response. Then, four subtypes called CD8-FRG subtypes were established under the combination of FRG signature and activity of CD8+ T-cells, which turned out to be effective in predicting the prognosis and immune therapeutic response of bladder cancer in multiple independent datasets. Pathway enrichment analysis, multiple gene signatures, and epigenetic alteration characterize the CD8-FRG subtypes and provide a potential combination strategy method against bladder cancer.

Conclusions:
In summary, the authors established a novel FRGI and CD8-FRG subtype by large-scale datasets and organised analyses, which could accurately predict clinical outcomes and immune therapeutic response of BLCA after surgery.
Efficacy and safety of therapies related to acupuncture for acute herpes zoster: A PRISMA systematic review and network meta-analysis
Authors: Liang XingYu , Chen Xi , Li XueMei , Yang Sha , Wang SiYing , Ma DanDan , Guo MingMing , Zhang Hong 
Published: May 2024

Background:
Herpes zoster (HZ) is mainly characterized by intense pain and severe skin lesions, particularly during the acute phase, which seriously affects the patient’s quality of life. Acupuncture is a widely used and effective treatment for HZ. However, there are many types of acupuncture, which have different curative efficacy. This study employed a network meta-analysis (NMA) to assess and rank the clinical efficacy of different acupuncture therapies.

Methods:
The database of Cochrane Library, Web of Science, PubMed, MEDLINE, Embase, China National Knowledge Infrastructure (CNKI), Chinese BioMedical Database, VIP Database, and Wanfang Database were searched from inception to December 31, 2022 to identify eligible randomized controlled trials (RCTs) of acupuncture related therapies in the treatment of acute HZ. The outcome indicators measured were visual analogue scale (VAS), date of cessation of herpes increase (DCHI), effective rate (ER), postherpetic neuralgia (PHN), and adverse events (AEs). Bayesian network meta-analyses were performed using the GeMTC package (version 1.0-1) and R software (version 4.2.3).

Results:
A total of 59 RCTs with 3930 patients were included. The results of this NMA were as follows: compared with pharmacotherapy, electroacupuncture (EA) + pricking and cupping (PC) shown the best efficacy to improve VAS score and reduce DCHI. In terms of ER, EA + fire needle (FN) had the highest results of probability ranking. PC was more effective in reducing the incidence of PHN. Furthermore, this study shown that the incidence of AEs associated with acupuncture-related therapies was acceptable.

Conclusions:
This study indicated that therapies related to acupuncture were both effective and safe in treating acute HZ, and could significantly reduce patients’ symptoms such as pain and skin lesions with fewer adverse events. Clinically, the selection of the appropriate therapy should be based on practical considerations. However, due to the limitations of this study, more high-quality trials are required to evaluate the efficacy and safety of acupuncture-related therapy for the treatment of acute HZ.
Case of acute retinal necrosis with rapid progression to proliferative vitreoretinopathy: A case report
Authors: Chujo Shinichiro , Matsubara Hisashi , Matsui Yoshitusgu , Kato Kumiko , Kondo Mineo 
Published: May 2024

Rationale:
Acute retinal necrosis (ARN) was first reported in 1971 by Urayama et al as an acute uveitis accompanied by retinal arteritis and white retinal lesions in the peripheral retina that can progress to a rhegmatogenous retinal detachment (RRD). We have experienced a case of ARN that, unlike the common developmental course to an RRD associated with ARN, progressed to proliferative vitreoretinopathy (PVR) involving the entire retina in 2 days. The purpose of this report is to present our findings in the case of ARN with an atypical rapid time course.

Patient concerns:
The patient was a 56-year-old woman who was treated for uveitis of unknown origin by her primary care physician. She was referred to our hospital because of a worsening of the fundus findings.

Diagnosis:
Fundus examination in our hospital revealed vitreous opacities in the right eye, yellowish-white lesions extending around the retina, and some retinal hemorrhages. Because the retinal changes suggested ARN, we performed a polymerase chain reaction of the anterior atrial fluid and detected varicella-zoster virus. Then, the diagnosis of ARN was confirmed, and treatment was begun. At 1 month and a half after beginning the treatment, focal retinal traction was observed in the right fundus. Two days later, a circumferential PVR and a total retinal detachment were detected.

Interventions:
We then performed vitrectomy with an encircling buckle and a silicone oil tamponade.

Outcomes:
Our examination 6 months postoperatively showed that the retina was attached and the BCVA was 20/200.

Lessons:
Our findings of a case of ARN showed that the progression from a local vitreous traction to a full circumferential PVR can develop in 2 days.
A novel discriminant algorithm for differential diagnosis of mild to moderate thalassemia and iron deficiency anemia
Authors: Pan Liqiu , Li Linlin , Qiu Yuling , Ling Xiaoting , Wang Chenghan , Wu Zuhao , Li Xiaoman , Lin Faquan , Huang Yifang 
Published: May 2024

Background:
Mild to moderate thalassemia trait (TT) and iron deficiency anemia (IDA) are the most common conditions of microcytic hypochromic anemia (MHA) and they exhibit highly similar clinical and laboratory features. It is sometimes difficult to make a differential diagnosis between TT and IDA in clinical practice. Therefore, a simple, effective, and reliable index is needed to discriminate between TT and IDA.

Methods:
Data of 598 patients (320 for TT and 278 for IDA) were enrolled and randomly assigned to training set (278 of 598, 70%) and validation set (320 of 598, 30%). Stepwise discriminant analysis was used to define the best diagnostic formula for the discrimination between TT and IDA in training set. The accuracy and diagnostic performance of formula was tested and verified by receiver operating characteristic (ROC) analysis in validation set and its diagnostic performance was compared with other published indices.

Results:
A novel formula, Thalassemia and IDA Discrimination Index (TIDI) = –13.932 + 0.434 × RBC + 0.033 × Hb + 0.025 ×MCHC + 53.593 × RET%, was developed to discriminate TT from IDA. TIDI showed a high discrimination performance in ROC analysis, with the Area Under the Curve (AUC) = 0.936, Youden’ s index = 78.7%, sensitivity = 89.5%, specificity = 89.2%, respectively. Furthermore, the formula index also obtained a good classification performance in distinguishing 5 common genotypes of TT from IDA (AUC from 0.854–0.987).

Conclusion:
The new, simple algorithm can be used as an effective and robust tool for the differential diagnosis of mild to moderate TT and IDA in Guangxi region, China.
Insomnia among college students: A bibliometric analysis from 2003 to 2022
Authors: Yang Mengdie , Li Lingling 
Published: May 2024

Background:
Insomnia has become a common health problem in modern society, especially among college students. The purpose of this study was to analyze the research status, research hotspots and frontier trends of insomnia among college students over the past 20 years.

Methods:
VOSviewer 1.6.19 and CiteSpace 6.2 were used. R4: This study conducts a bibliometric and visualization analysis of the annual publications, authors, countries/regions, institutions, categories, journal/literature cocitations and keywords related to insomnia among college students in the Web of Science (WoS) core collection from 2003 to 2022.

Results:
A total of 590 studies were included, and the number of studies on insomnia among college students has steadily increased over the last 20 years. The authors of high yield are represented by Taylor DJ and Miller MB. The countries/regions with high yields were the USA and China. The institutions of high yield were King Saud University and Southern Medical University. Its research fields were mainly Clinical Neurology, Psychiatry and Neurosciences. Mental health and insomnia, sleep quality and the impact of coronavirus disease 2019 (COVID-19) on insomnia are current research hotspots. Future research could focus on predicting the chronotype and physical activity of insomnia students.

Conclusion:
Through bibliometric and visualization analysis, this study investigated insomnia among college students over the past 20 years and preliminarily revealed the findings of coauthors and institutions. This study provides a general understanding of the research hotspots and frontier trends of insomnia among college students and provides some references for future research.
Effects of preoperative inhaled budesonide combined with intravenous dexamethasone on postoperative sore throat in patients who underwent thyroidectomy: A randomized controlled trial
Authors: Chen Ping-Ping , Zhang Xing , Ye Hui , Chen Dan 
Published: May 2024

Background:
This randomized controlled trial aimed to evaluate the efficacy of preoperative inhaled budesonide combined with intravenous dexamethasone on postoperative sore throat (POST) after general anesthesia in patients who underwent thyroidectomy.

Methods:
Patients who underwent elective thyroidectomy were randomly divided into the intravenous dexamethasone group (group A) and budesonide inhalation combined with intravenous dexamethasone group (group B). All patients underwent general anesthesia. The incidence and severity of POST, hoarseness, and cough at 1, 6, 12, and 24 hours after surgery were evaluated and compared between the 2 groups.

Results:
There were 48 and 49 patients in groups A and B, respectively. The incidence of POST was significantly lower at 6, 12, and 24 hours in group B than that in group A (P < .05). In addition, group B had a significantly lower incidence of coughing at 24 hours (P = .047). Compared with group A, the severity of POST was significantly lower at 6 (P = .027), 12 (P = .004), and 24 (P = .005) hours at rest, and at 6 (P = .002), 12 (P = .038), and 24 (P = .015) hours during swallowing in group B. The incidence and severity of hoarseness were comparable at each time-point between the 2 groups (P > .05).

Conclusion:
Preoperative inhaled budesonide combined with intravenous dexamethasone reduced the incidence and severity of POST at 6, 12, and 24 hours after extubation compared with intravenous dexamethasone alone in patients who underwent thyroidectomy. Additionally, this combination decreased the incidence of postoperative coughing at 24 hours.
Investigating the molecular mechanism of Mori Cortex against osteosarcoma by bioinformatics analysis and in vitro experimental
Authors: Wang Yuanhui , Wang Ling , Xie Dongke , Chen Bo 
Published: May 2024

Objective:
To explore the therapeutic mechanism of Mori Cortex against osteosarcoma (OS), we conducted bioinformatics prediction followed by in vitro experimental validation.

Methods:
Gene expression data from normal and OS tissues were obtained from the GEO database and underwent differential analysis. Active Mori Cortex components and target genes were extracted from the Traditional Chinese Medicine System Pharmacology database. By intersecting these targets with differentially expressed genes in OS, we identified potential drug action targets. Using the STRING database, a protein-protein interaction network was constructed. Subsequent analyses of these intersected genes, including Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment, were performed using R software to elucidate biological processes, molecular functions, and cellular components, resulting in the simulation of signaling pathways. Molecular docking assessed the binding capacity of small molecules to signaling pathway targets. In vitro validations were conducted on U-2 OS cells. The CCK8 assay was used to determine drug-induced cytotoxicity in OS cells, and Western Blotting was employed to validate the expression of AKT, extracellular signal-regulated kinases (ERK), Survivin, and Cyclin D1 proteins.

Results:
Through differential gene expression analysis between normal and OS tissues, we identified 12,364 differentially expressed genes. From the TCSMP database, 39 active components and 185 therapeutic targets related to OS were derived. The protein-protein interaction network indicated that AKT1, IL-6, JUN, VEGFA, and CASP3 might be central targets of Mori Cortex for OS. Molecular docking revealed that the active compound Morusin in Mori Cortex exhibits strong binding affinity to AKT and ERK. The CCK8 assay showed that Morusin significantly inhibits the viability of U-2 OS cells. Western Blot demonstrated a reduction in the p-AKT/AKT ratio, the p-ERK/ERK ratio, Survivin, and Cyclin D1.

Conclusion:
Mori Cortex may exert its therapeutic effects on OS through multiple cellular signaling pathways. Morusin, the active component of Mori Cortex, can inhibit cell cycle regulation and promote cell death in OS cells by targeting AKT/ERK pathway.
Geographic and Racial/Ethnic Differences in Access to Methamphetamine Detoxification Services, United States, 2021
Authors: Pro George , Cantor Jonathan , Buttram Mance , Brown Clare C. , Gu Mofan , Mancino Michael , Zaller Nickolas 
Published: May 2024

Introduction:
Methamphetamine detoxification before entering formal and longer term treatment may have a positive impact on treatment retention and success. Understanding geographic distribution of methamphetamine specialty detox services and differential access by race/ethnicity is critical for establishing policies that ensure equitable access across populations.

Methods:
We used the Mental health and Addiction Treatment Tracking Repository to identify treatment facilities that offered any substance use detoxification in 2021 (N=2346) as well as the census block group in which they were located. We sourced data from the US Census Bureau to identify the percentage of a census block group that was White, Black, and Hispanic. We used logistic regression to model the availability of methamphetamine-specific detox, predicted by the percentage of a block group that was Black and Hispanic. We adjusted for relevant covariates and defined state as a random effect. We calculated model-based predicted probabilities.

Results:
Over half (60%) of detox facilities offered additional detox services specifically for methamphetamine. Sixteen states had <10 methamphetamine-specific detox facilities. The predicted probability of methamphetamine-specific detox availability was 60% in census block groups with 0%–9% Black residents versus only 46% in census block groups with 90%–100% Black residents, and was 61% in census block groups with 0%–9% Hispanic residents versus 30% in census block groups with 90%–100% Hispanic residents.

Conclusions:
During an unprecedented national methamphetamine crisis, access to a critical health care service was disproportionately lower in communities that were predominately Black and Hispanic. We orient our findings around a discussion of health disparities, residential segregation, and the upstream causes of the systematic exclusion of minoritized communities from health care.
Effects of botulinum toxin type A in patients with painful temporomandibular joint disorders: a systematic review and meta-analysis
Authors: Zhu Mengjiao , Huang Ziwei , Wang Yeye , Qin Jing , Fan Mingyue 
Published: May 2024

Objective:
To assess the therapeutic efficacy of botulinum toxin type A (BTX-A) for managing myofascial pain related to temporomandibular disorders (TMDs).

Methods:
This study was conducted according to the PRISMA 2020 statement guidelines. The PubMed, Embase, and Cochrane Library databases were searched. Only randomized controlled trials were included. The primary outcome was a pain score on the visual analog scale, and the secondary outcomes were maximum mouth opening and adverse effects. The Cochrane risk of bias tool was used to assess risk bias. A meta-analysis of studies with the same interventions, controls, assessment methods, and follow-up durations was performed.

Results:
A total of 519 studies were retrieved, of which 20 randomized controlled trials were included in the qualitative analysis and six were included in the meta-analysis. The results showed that, compared with placebo, BTX-A injection was more effective at relieving myofascial pain, and its effect was similar to that of conventional methods. However, there was no difference in maximum mouth opening between the two groups. After the study assessment with the RoB 2.0 tool, six studies showed a low risk of bias, 13 studies showed some concerns regarding the reported results, and only one study showed a high risk of bias. Adverse effects of BTX-A injection were observed in four studies.

Conclusions:
In conclusion, BTX-A is effective at relieving pain in TMD patients but does not improve mouth opening. To minimize adverse effects, we recommend a low dose of BTX-A for TMD patients who do not experience complete pain relief from conservative treatments.