bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Cry1Ac and Cry2Ab toxins on the development of the predator Chrysoperla zastrowi sillemi (Esben-Petersen) through prey-mediated tri-trophic interaction analysis
Authors: Kaur Jagdeep , Aggarwal Naveen 
Published: June 2024

Abstract

Adopting genetically modified crops in developing nations like India has consistently been hindered by apprehensions regarding their impact on non-target arthropods. Consequently, laboratory experiments were conducted to evaluate the impact of Bt toxins of BG-II cotton on the common green lacewing [Chrysoperla zastrowi sillemi (Esben-Petersen)], by exposing them indirectly to the toxins. Prey-mediated feeding bioassays, utilizing BG-II cotton and non-Bt cotton plants as hosts, two prey herbivores, specifically Aphis gossypii Glover and Bemisia tabaci (Gennadius), were conducted on several days to assess the effects of Cry toxins on the growth of C. z. sillemi. The ELISA assay, quantified the Cry toxins across three trophic levels and revealed that the leaves had the highest quantities of Cry1Ac and Cry2Ab toxins, measuring 7.36 and 23.54 µg/g FW, respectively. In contrast, herbivores and predators exhibited very low concentrations of Cry toxins. Although traces of Cry toxins were found in both herbivores and predators, they had no negative impacts on any of the predator's developmental characteristics, confirming that these toxins do not threaten C. z. sillemi.
Light-Mediated Elicitation of Tessaria absinthioides Calli for the Production of Anthocyanins: Correlation with Protein Expression
Authors: Martinez Antares , Bonilla José , Aguilera Leonardo , Callegari Eduardo , Paez Maria Daniela , Kurina-Sanz Marcela , Orden Alejandro Agustin 
Published: June 2024

Abstract

This study explores the metabolic responses of undifferentiated cells of Tessaria absinthioides to light elicitation, with a focus on the production and accumulation of anthocyanins. We observed that anthocyanin concentrations in cell biomass were notably higher compared to those in wild flowers, albeit with slight compositional differences, according to the HPLC-DAD analysis. In a proteomic analysis of T. absinthioidescalli, light exposure induced significant changes in protein expression, with 578 proteins overexpressed and 486 uniquely identified under this condition. These proteins were predominantly clustered in key functional categories like protein processing, cellular transport, and DNA regulation, along with those involved in sesquiterpene synthesis and developmental regulation. Furthermore, proteins crucial for the phenylpropanoid pathway and the anthocyanin biosynthesis were identified in light-exposed calli, with many significantly overexpressed compared to dark-grown cultures. A similar trend was observed in enzymes associated with the de novo synthesis of arginine, a precursor to stress-responsive metabolites like polyamines and nitric oxide. These findings help elucidate the metabolic pathways underlying anthocyanin production in T. absinthioides. They also extend to the development of natural dyes, highlighting the significance of cellular culture in sustainable bioproduction.
A case report of angioimmunoblastic T-cell lymphoma developing into B-cell precursor acute lymphoblastic leukemia after autologous hematopoietic stem cell transplantation
Authors: Zhang Yanyu , Zhang Lizhi , Shen Jingzhi , Sun Xiuli 
Published: June 2024

Abstract

Angioimmunoblastic T-cell lymphoma (AITL) is recognized as an independent clinicopathological entity that often presents with immune imbalance and a hyperinflammatory response. In addition to neoplastic T-cell clones, AITL is frequently accompanied by dominant B-cell clones. B-cell precursor acute lymphoblastic leukemia (BCP-ALL), a rarer class of B-ALL, associates with a poor prognosis in adults. Previous reports have often mentioned cases of B-cell lymphoma secondary to AITL, but no cases of secondary B-ALL have been reported. In this manuscript, we present a case of the patient with AITL who developed secondary BCP-ALL following autologous hematopoietic stem cell transplantation (AHSCT). Remarkably, the patient demonstrate durable complete remission after dose-reduced chemotherapy.
Triglyceride to High-Density Lipoprotein ratio as a predictor for 10-year cardiovascular disease in individuals with diabetes in Thailand
Authors: Poochanasri Methavee , Lertsakulbunlue Sethapong , Kookanok Chutawat , Rangsin Ram , Kaewput Wisit , Mungthin Mathirut , Samakkarnthai Parinya 
Published: June 2024

Abstract

Background
Cardiovascular disease (CVD) represents a group of disorders affecting the heart and blood vessels. The Framingham Heart Study (FHS) predicted a 10-year CVD risk score is used to establish risk stratification and evaluate intervention outcomes. TG/HDL ratio is utilized as an alternative tool to assess CVD risk. This study, therefore, aims to investigate the role of the TG/HDL ratio in predicting the 10-year cardiovascular risk utilizing the established FHS risk prediction model in individuals with T2DM.
Methods
A cross-sectional study was conducted among 61,004 patients with T2DM without a history of CVD aged between 30–74 years. From 2014, 2015, and 2018. The predicted 10-year CV risk was calculated using laboratory-based Framingham heart study equations. The cut point of TG/HDL was calculated using Youden’s J statistics of the ROC curve for predicting high predicted 10-year CV risk. Linear and Multivariable logistic regression analyses were performed to investigate the association between TG/HDL and high predicted 10-year CVD risk.
Results
For the overall participant group, ROC curve analysis yielded an AUC of 0.62 (95% CI 0.61–0.62) for predicting high FHS-CVD risk, with a TG/HDL cutoff value of 3.09 (Sensitivity 61%, Specificity 57%). Linear regression analysis revealed a dose-response relationship between the TG/HDL ratio and log-transformed predicted 10-year CV risk across both sexes and all age groups. Participants with high TG/HDL ratios (> 3.09) were positively associated with a prevalence of high FHS-CVD risk (AOR: 5.91, 95% CI 5.52–6.31). In comparison with participants in the first TG/HDL quartile (< 2.05), those in the fourth quartile (> 4.91) had a higher chance of high predicted 10-year CV risk (AOR: 18.27, 95% CI 16.537–20.185). Subgroup analysis revealed that the strength of association was more intense in females and those of advanced age.
Conclusions
TG/HDL can be used as a risk factor to determine predicted 10-year CV risk, particularly in individuals with T2DM aged between 30–74 years. However, further study is necessary to robustly establish the association between TG/HDL and cardiovascular disease risk prediction.
Integration of an Implantable Biosensor within a Traditional Oral Bite Guard for Bruxism Detection: An In Vitro Study
Authors: Al-Hamad Khalid , Asiri Ashwaq , Al-Qahtani Ali , Alotaibi Saud , Almalki Abdullah 
Published: June 2024

Abstract

While biofeedback therapy based on occlusal force has demonstrated safety, effectiveness, and minimal adverse effects in bruxism treatment, the underlying mechanism and fundamental technologies remain elusive. This study aimed to implement a biosensor device into a conventional bite night guard to detect bruxism. A layering process (sandwich technique) was utilized to integrate stress sensors, which were then inserted into an acrylic occlusal stabilization splint. The core components of the sensor system consisting of a server terminal, a core control module, and a "pressure sensor data acquisition unit". The occlusal force data analysis and parameter setup employed a machine learning technique. A prototype sensor platform was meticulously developed to assess each aspect of the smart splint comprehensively. Experimental outcomes substantiated the viability of the intended approach for teeth-grinding therapy and unveiled appropriate parameter metrics for the sensor system
Nanopore sequencing-based genotyping corroborated the association between CYP2D6 function and susceptibility to anxiety and depression
Authors: Chua Eng Wee , Karunanathie Harsheni , Yanasegaran Kevina , Maggo Simran , Ng Pei Yuen , Kee Ping Siu , Kennedy Martin , Manaf Mohd Rizal Abdul 
Published: June 2024

Abstract

Objective
CYP2D6 activity has been inconsistently associated with anxious and depressive personality traits. The inconsistency may stem from limitations of targeted genotyping, employed in most previous studies, leading to undetected errors in metabolic classification. Using a nanopore sequencing-based method, we comprehensively genotyped CYP2D6 alleles in a small cohort of 96 Malaysians and re-examined the relationship between CYP2D6 activity and susceptibility to anxiety and depression.
Results
In keeping with prior studies, CYP2D6*10 was found to be the most common defective allele; nearly half of the (48.5%) participants were classified as intermediate and poor metabolisers. Linear regression analysis revealed that impaired CYP2D6 activity was a significant predictor of anxiety and depression, consistent with the putative role of CYP2D6 in the synthesis of serotonin and dopamine, the mood-boosting neurotransmitters. We hope that this brief report will prompt larger-scale studies to further elucidate the contribution of CYP2D6 to the genetic underpinnings of mental well-being.
Across a phylogeographic break in the Qinling Mountains - Huaihe River Line :Quaternary evolutionary history of a medicinal and edible homologous plants (Allium macrostemon) in China
Authors: Jiang Chunxue , Shi Tian , Mo Zhongmei , Zhao Cai 
Published: June 2024

Abstract

Biogeographical barriers to gene flow are central to plant phylogeography. There are many physical and geographic boundaries in China, but there are few studies on the natural geographical isolation boundary of the Qinling Mountains-Huaihe River Line (QHL) using molecular ecological evidence. Allium macrostemon is a perennial herb belonging to the family Amaryllidaceae. It is a precious Chinese herb with the same origin as medicine and food, and has a variety of health and healing properties. Five SSR markers, three chloroplast DNA (cpDNA) markers (psbA-trnH, rps16 and trnL-F), one nuclear ribosomal DNA (nrDNA) marker (ITS), and simplified genome GBS sequencing were used to analyze the genetic diversity and genetic structure of A. macrostemon. Combining SSR, cpDNA, nrDNA ITS data and GBS analysis results, A. macrostemon populations could be grouped into northern and southern group, with the southern group further divided into southwestern and central-southeastern group. Niche simulations results show that the distribution area of A. macrostemon will reach its maximum in the future. These data indicate that a geographical barrier and Quaternary climate have maintained the regional separation of A. macrostemon, and the back-and-forth fluctuations of QHL and Quaternary climate play an important role in this process. QHL acts as a north-south dividing line in phylogeography and population genetic structure, promoting physical geographic isolation. This study can provide scientific theoretical basis for the conservation, development and utilization of A. macrostemon resources, further provide reference for the systematic geographical pattern of large-scale spatial distribution of plants in China, and enrich our understanding of the Quaternary plant evolution history in complex terrain areas.
Perceptions about dementia clinical trials among underrepresented populations: A nationally representative survey of U.S. dementia caregivers
Authors: Leggins Brandon , Hart Danielle M. , Jackson Ashley J. , Levenson Robert W. , Windon Charles C. , Merrilees Jennifer , Chiong Winston 
Published: June 2024

Abstract

Background: The research community has historically failed to enroll diverse groups of participants in dementia clinical trials. A unique aspect of dementia care research is the requirement of a study partner, who can attest to the care recipient’s clinical and functional capacity. The aim of this study is to assess racial and ethnic differences and the importance of various trial considerations among dementia caregivers, in their decision to participate in clinical research as study partners.Method: We embedded a vignette about a hypothetical dementia clinical trial in a nationally representative survey of U.S. dementia caregivers, oversampling non-Hispanic Black and Hispanic caregivers. Dementia caregivers were asked about their willingness to participate in the trial with their care recipient and rated the importance of nine considerations in hypothetical decisions to participate. Caregiver demographic characteristics were analyzed as predictors of trial participation in a base demographic model. In a second reasons model caregiver demographic characteristics and the rated importance of the nine considerations were separately analyzed as predictors; both models used survey-weighted logistic regression.Result: The sample consisted of 610 dementia caregivers, including 156 non-Hispanic Black and 122 Hispanic caregiver participants. In the base demographic model, hypothetical trial participation was negatively associated with older caregiver age (OR (odds ratio) = 0.72, p = < 0.001). In the reasons model, the rated importance of a social responsibility to help others by participating in research was significantly associated with participation (OR = 1.56, p = 0.049), while the importance of the possibility of the care recipient experiencing serious side effects was negatively associated with participation (OR = 0.51, p = 0.003). In both models there was no significant difference in hypothetical participation between non-Hispanic Black and non-Hispanic White caregivers, or between Hispanic and non-Hispanic White caregivers.Conclusion: Hispanic and non-Hispanic Black dementia caregivers were not less likely than non-Hispanic White dementia caregivers to participate in a hypothetical dementia clinical trial. Our study suggests that failures to recruit diverse populations in dementia clinical research are not attributable to less willingness among members of underrepresented groups but may instead reflect structural barriers and historic exclusion from trial participation.
Construct a Refined Monte Carlo Model for BNCT Dose Calculation and Assessment by Converting HU Value to Material Compositions for ROIs
Authors: Teng Yi-Chiao , Chen Jiang , Zhong Wan-Bing , Liu Yuan-Hao 
Published: June 2024

Abstract

The work of general Hounsfield unit (HU) value conversion material library has a prominent advantage in reflecting the multiplicity of tissue material compositions. For the sake of conquering the impact of limitations of imaging energy resolution and spatial resolution, while bearing in mind the accuracy and conservatism of BNCT dose calculation and assessment, a HU conversion material library for organs at risk (OAR) is established. The region of interest (ROI) is assigned to the OAR material library to build Monte Carlo model, which can be evolved into a homogeneous material with a single composition or a heterogeneous material with multiple compositions. A benchmark comparison of a coarse model with conventional fixed material library versus a refined model of HU-based converting approach coupled with an improved OAR-related ingrained material library within ROI was performed on practical glioma and head-and-neck tumor cases. Comparing the refined model with the coarse model showed that the minimum bioequivalent dose rate and physical absorbed dose rate of tumor differed by more than 3.6%, the health tissue maximum bioequivalent dose rate differed by 12.9%, and the maximum physical absorbed dose rate of the health tissue differed by 5.9%. Elemental compositions and mass densities influence the dose distribution. Delicately defined material compositions should be applied to ensure the trustworthiness of the calculated dose. Taking into account individual patient differences, improved material modeling strategies allow for simulations that are closer to the patient’s authentic physical condition, thereby more accurately assessing health tissue dose limit and tumor prescribed dose.
Hydroxychloroquine in the treatment of sarcoidosis-associated uveitis and idiopathic uveitis
Authors: PLAVONIL Sarah , EL-JAMMAL Thomas , JACQUOT Robin , BERT Arthur , JAMILLOUX Yvan , KODJIKIAN Laurent , SEVE Pascal 
Published: June 2024

Abstract

Background : Uveitis is one of the five leading causes of blindness in developed countries, and adverse effects associated with long-term use of topical or systemic steroids and immunosuppressive agents are notable. The objective was to evaluate the efficacy and safety of hydroxychloroquine (HCQ) in sarcoidosis-associated uveitis (SAU) and idiopathic uveitis (IU).
Methods: Monocentric retrospective study including 42 patients with SAU and 15 patients with IU treated with HCQ for at least 6 months between March 2003 and December 2022. The main objective was to determine the success rate of the HCQ at 6 and 12 months, and at the last visit, and was defined as having control of inflammation, no more than 5 mg prednisone daily and less than or equal to 2 drops of dexamethasone phosphate 0.1%, and no treatment failure due to safety. The Fisher’s exact test and the Wilcoxon rank test were used for the comparison of qualitative data and quantitative data respectively. Prednisone dose was compared using a mixed model.
Results: The median duration to the last visit was 19.5 [11; 44.8] months in SAU patients and 18 [13; 38] months in IU patients. At the last visit, 55% patients with SAU (including 71% anterior SAU and 100% intermediate SAU) and 40% patients with IU (including 40% anterior IU) were successfully treated with HCQ ; the median prednisone dose decreased from 10 [IQR, 8.0; 27.5] to 4 [IQR, 2.5; 5.75] mg/d and from 15.5 [IQR, 12.5; 19.5] to 3.0 [IQR, 3.0; 5.0] mg/d in SAU and IU patients, respectively. The reduction in median prednisone dose was significant in patients with SAU (p=0.002). The incidence rate ratio of flare was 0.73 (p=0.143) in SAU patients and 0.26 (p < 0.001) in IU patients.
Conclusion: HCQ could be an interesting therapeutic option in certain types of SAU and IU. Additionally, HCQ decreased the incidence of flare-ups and the need for oral prednisone in these patients.
Trial registration : The present study received approval from the local ethics committee (CPP Sud Est IV) in February 2019 (N° 19-31) and was registered on www.clinicaltrials.gov (NCT03877575).
Exploring why and when workplace friendship has a differentiated effect on relationship norms and helping behavior: A relationship motivation theory approach
Authors: Wang Shuai , Jiao Guanzhe , Chen Yun 
Published: June 2024

Abstract

Although scholars have begun to focus on the complexity of workplace friendship as a collection of exchange relationships and communal relationships that contain conflicting exchange norms and communal norms (Pillemer & Rothbard, 2018), unfortunately, our elaborated understanding of whether the differentiated effects of workplace friendship on organizational citizenship behavior or not due to such complexity and, especially, boundary conditions for their differentiated influence are limited. Our purpose is to develop a motivational framework that reveals when and how differentiated helping is driven by workplace friendship via different relationship norms considering perceived relationship motivations. To do so, we argue that workplace friendship can lead to proactive helping and reactive helping through adherence to communal norms and exchange norms, respectively, and such a differentiated effect of workplace friendship hinges on employees’ perceived partners’ relationship motivation. Using multi-wave survey (Study 1) and experiment (Study 2) methods, we found that when employees perceive others are driven by autonomous relationship motivation, workplace friendship is positively related to communal norms, enhancing proactive helping, whereas when employees perceive others are driven by controlled relationship motivation attribution, workplace friendship is positively related to exchange norms, enhancing reactive helping. Our study contributes to the literature on workplace friendship and relationship motivation.
Normative age and gender differences in lie-telling across early and mid-adolescence from a multi-informant perspective
Authors: Gouwy Marie-Céline , Dierckx Kim , Ivanova Masha , Roets Arne , De Clercq Barbara 
Published: June 2024

Abstract

The present study investigates age and gender differences in lie-telling during the sensitive developmental phase of adolescence, using a multi-informant approach. Data from a large, multi-national dataset was used, including 25,643 participants aged 11–18 years across 24 societies, using the Child Behavior Checklist (CBCL) and Youth Self-Report (YSR). Mixed models were applied to examine both linear and curvilinear age trends, gender differences, and discrepancies between self- and parental reports on lying. The findings revealed that boys consistently showed higher frequencies of lying across all ages and informants, highlighting a robust gender difference. Also, significant mean-level age trends in lying were found, which however differed across informants. Adolescents’ reporting showed an inverted U-shape pattern in lying frequency with growing age, peaking around mid-adolescence, while parental reports showed a U-shaped trend in their child’s lying frequency across age, although curvilinearity was less pronounced. The highest discrepancy between self- and parental reports of adolescent lying was situated in mid-adolescence, likely due to both a parental truth bias and the increased sophistication of adolescents in concealing their lies. These results suggest that informant agreement during mid-adolescence on rather undesirable behavior may be particularly challenging. These informant discrepancies on lying may also have clinical significance, and future research may further address to what extent each of these informant perspectives generate relevant information to understand mental health outcomes in youth.