bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalidation of Salivary Biomarkers for Oral Cancer Detection
Authors: Elashoff David , Zhou Hui , Reiss Jean , Wang Jianghua , Xiao Hua , Henson Bradley , Hu Shen , Arellano Martha , Sinha Uttam , Le Anh , Messadi Diana , Wang Marilene , Nabili Vishad , Lingen Mark , Morris Darly , Randolph Timothy , Feng Ziding , Akin David , Kastratovic Dragana A. , Chia David , Abemayor Elliot , Wong David T.W. 
Published: March 2012

Abstract
Background: Oral cancer is the sixth most common cancer with a 5-year survival rate of approximately 60%. Presently, there are no scientifically credible early detection techniques beyond conventional clinical oral examination. The goal of this study is to validate whether the seven mRNAs and three proteins previously reported as biomarkers are capable of discriminating patients with oral squamous cell carcinomas (OSCC) from healthy subjects in independent cohorts and by a National Cancer Institute (NCI)-Early Detection Research Network (EDRN)-Biomarker Reference Laboratory (BRL).
Methods: Three hundred and ninety-five subjects from five independent cohorts based on case controlled design were investigated by two independent laboratories, University of California, Los Angeles (Los Angeles, CA) discovery laboratory and NCI-EDRN-BRL.
Results: Expression of all seven mRNA and three protein markers was increased in OSCC versus controls in all five cohorts. With respect to individual marker performance across the five cohorts, the increase in interleukin (IL)-8 and subcutaneous adipose tissue (SAT) was statistically significant and they remained top performers across different cohorts in terms of sensitivity and specificity. A previously identified multiple marker model showed an area under the receiver operating characteristic (ROC) curve for prediction of OSCC status ranging from 0.74 to 0.86 across the cohorts.
Conclusions: The validation of these biomarkers showed their feasibility in the discrimination of OSCCs from healthy controls. Established assay technologies are robust enough to perform independently. Individual cutoff values for each of these markers and for the combined predictive model need to be further defined in large clinical studies.
Impact: Salivary proteomic and transcriptomic biomarkers can discriminate oral cancer from control subjects. Cancer Epidemiol Biomarkers Prev; 21(4); 664–72. ©2012 AACR.
Night Shift Work and Hormone Levels in Women
Authors: Davis Scott , Mirick Dana K. , Chen Chu , Stanczyk Frank Z. 
Published: March 2012

AbstractBackground: Night shift work may disrupt the normal nocturnal rise in melatonin, resulting in increased breast cancer risk, possibly through increased reproductive hormone levels. We investigated whether night shift work is associated with decreased levels of urinary 6-sulfatoxymelatonin, the primary metabolite of melatonin, and increased urinary reproductive hormone levels.Methods: Participants were 172 night shift and 151 day shift–working nurses, aged 20–49 years, with regular menstrual cycles. Urine samples were collected throughout work and sleep periods and assayed for 6-sulfatoxymelatonin, luteinizing hormone (LH), follicle–stimulating hormone (FSH), and estrone conjugate (E1C).Results: 6-Sulfatoxymelatonin levels were 62% lower and FSH and LH were 62% and 58% higher, respectively, in night shift–working women during daytime sleep than in day shift–working women during nighttime sleep (P ≤ 0.0001). Nighttime sleep on off-nights was associated with 42% lower 6-sulfatoxymelatonin levels among the night shift workers, relative to the day shift workers (P &lt; 0.0001); no significant differences in LH or FSH were observed. 6-Sulfatoxymelatonin levels during night work were approximately 69% lower and FSH and LH were 35% and 38% higher, compared with day shift workers during nighttime sleep. No differences in E1C levels between night and day shift workers were observed. Within night shift workers, 6-sulfatoxymelatonin levels were lower and reproductive hormone levels were higher during daytime sleep and nighttime work, relative to nighttime sleep (P &lt; 0.05).Conclusions: These results indicate that night shift workers have substantially reduced 6-sulfatoxymelatonin levels during night work and daytime sleep and that levels remain low even when a night shift worker sleeps at night.Impact: Shift work could be an important risk factor for many other cancers in addition to breast cancer.MeSH Subject Headings: breast cancer, shift work, circadian rhythm, environmental carcinogens, estrogen, melatonin, 6-sulfatoxymelatonin, pineal. Cancer Epidemiol Biomarkers Prev; 21(4); 609–18. ©2012 AACR.
The Impact of Anemia on Long-Term Clinical Outcome in Patients Undergoing Revascularization With the Unrestricted Use of Drug-Eluting Stents
Authors: Pilgrim Thomas , Vetterli Florian , Kalesan Bindu , Stefanini Giulio G. , Räber Lorenz , Stortecky Stefan , Gloekler Steffen , Binder Ronald K. , Wenaweser Peter , Moschovitis Aris , Khattab Ahmed A. , Buellesfeld Lutz , Zwahlen Marcel , Meier Bernhard , Jüni Peter , Windecker Stephan 
Published: March 2012

Background—
Anemia is frequent among patients with cardiovascular disease and adversely affects prognosis. The objective of this analysis was to assess the impact of anemia on long-term clinical outcomes among patients undergoing percutaneous coronary intervention (PCI) with the unrestricted use of drug-eluting stents (DES).

Methods and Results—

Between April 2002 to March 2009, 6528 consecutive patients underwent PCI with the unrestricted use of DES. Among patients with anemia according to the criteria by the World Health Organization (WHO) (hemoglobin <130 g/L for men and <120 g/L for women, respectively) patients below the 25
th
percentile separately for men and women were defined to have severe anemia. We compared clinical outcomes among patients with severe anemia and no/mild anemia during long-term follow-up through 4 years. Whereas 21.6% of patients were found to have anemia according to the WHO definition, 347 patients (5.5%) had severe anemia (mean hemoglobin, 98±11 g/L). Severe anemia was more prevalent among the elderly (
P
<0.001), diabetics (
P
<0.001), and patients with chronic kidney disease (
P
<0.001). In adjusted analyses, severe anemia was associated with an increased risk of death (hazard ratio, 1.86; 95% confidence interval, 1.37–2.52;
P
<0.0001), cardiac death (hazard ratio, 2.32; 95% confidence interval, 1.57–3.43;
P
<0.0001), and myocardial infarction (hazard ratio, 2.02; 95% confidence interval, 1.36–3.01;
P
=0.00054) as compared with no/mild anemia without significant interaction across sexes (
P
=0.86) and acute coronary syndromes (
P
=0.61) and a trend toward a particularly high risk of mortality among anemic patients <65 years of age (
P
=0.07). Severe anemia resulted in a greater risk of overall definite stent thrombosis (hazard ratio, 2.59; 95% confidence interval, 1.48–4.54;
P
=0.00089).

Conclusions—
Severe anemia is common among patients undergoing PCI with the unrestricted use of DES and adversely affects long-term prognosis, including survival.
Differential cellular and microbial responses to nano-/micron-scale titanium surface roughness induced by hydrogen peroxide treatment
Authors: Daw Abdulaziz E , Kazi Hizbullah AA , Colombo John S , Rowe Wendy G , Williams David W , Waddington Rachel J , Thomas David W , Moseley Ryan 
Published: March 2012

This study investigated the effects of combined titanium nano-/micron-scale roughness, induced by hydrogen peroxide pre-treatments, on bone marrow stromal cell responses and Porphyromonas gingivalis adherence in vitro. Untreated surfaces exhibited nano-scale features, while hydrogen peroxide treatments promoted increased nano-/micron-scale roughness. Bone marrow stromal cell attachment and proliferation were maintained with 6 h and 24 h treatments, but significantly decreased on 1-week and 4-week-treated surfaces. Bone marrow stromal cells on 6 h-4 week-treated titanium demonstrated enhanced osteogenic differentiation versus untreated surfaces. P. gingivalis adherence was significantly increased on 24 h–4 week surfaces. Results suggest that 6 h but less than 24 h treatments maintain or promote bone marrow stromal cell responses while minimizing microbial adherence, potentially enhancing titanium surface bio-activation for osseointegration.
In vivo chondrocyte and transforming growth factor-β1 delivery using the thermosensitive chitosan/starch/β-glycerol phosphate hydrogel
Authors: Faikrua Atchariya , Wittaya-areekul Sakchai , Oonkhanond Bovornlak , Viyoch Jarupa 
Published: March 2012

In present study, the chitosan/starch/β-glycerol phosphate hydrogel was investigated as an effective carrier for chondrocytes and delivery of transforming growth factor-β1. In vitro study indicated that transforming growth factor-β1 was released sustainably for 14 days with its biological activity to stimulate chondrocyte functions, as indicated by the strong expression of type II collagen protein. Subcutaneous implantation to rats revealed the strong expressions of type II collagen and aggrecan messenger ribonucleic acids, and also type II collagen protein was observed in the hydrogel in combination with transforming growth factor-β1 within 2 weeks. Our collective results showed the potential of chitosan/starch/β-glycerol phosphate hydrogel for effective delivery of chondrocytes and transforming growth factor-β1, and preserve chondrocytes’ phenotype and functions in vitro.
Pattern-based formulation: a methodology for psychiatric case formulation
Authors: Fernando Irosh , Cohen Martin , Henskens Frans 
Published: March 2012

Objective: Psychiatric formulation is often considered a challenging task, particularly for trainees and students in psychiatry. The process of arriving at a good psychiatric formulation involves identifying major issues of the patient, and developing an explanatory system, which can be derived using known theoretical models in psychiatry. A good explanatory system should provide a high degree of coherence by linking the most relevant data, justification for the proposed management plan, and its feasibility. Unfortunately, the literature on ‘how’ to develop psychiatric formulation is limited, and for the trainees, psychiatric formulation remains a challenging task. Method: Based on the clinical reasoning strategy known as pattern recognition, a methodology for psychiatric case formulation has been proposed. The methodology uses a repository of templates, which are derived from major theoretical models in psychiatry. Developing a formulation involves a process of selecting, prioritising and combining templates according to the elicited set of main issues or psychopathology. The pattern resulting from the combination of different templates provides a degree of uniqueness to the explanatory system. Conclusion: It is expected that this methodology will help trainees and students to develop a broader understanding and skills in psychiatric formulation.
The myth of Jocasta and maternal narcissism
Authors: Butterfield Ingrid 
Published: March 2012

Objective: This paper utilises the ancient Greek myth of Oedipus, and in particular the character’s frequently overlooked mother Jocasta, to highlight some of the issues associated with maternal narcissism. Conclusions: A discussion of Jocasta forms the background to case studies where a mother’s narcissism, along with an absent father, was pivotal to coping difficulties, which ultimately compelled the patient to seek therapy. Consideration of the Oedipus–Jocasta interpersonal dynamic can contribute to a greater understanding of these patients.
A genome-wide association study in progressive multiple sclerosis
Authors: Martinelli-Boneschi Filippo , Esposito Federica , Brambilla Paola , Lindström Eva , Lavorgna Giovanni , Stankovich Jim , Rodegher Mariaemma , Capra Ruggero , Ghezzi Angelo , Coniglio Gabriella , Colombo Bruno , Sorosina Melissa , Martinelli Vittorio , Booth David , Oturai Annette Bang , Stewart Graeme , Harbo Hanne F. , Kilpatrick Trevor John , Hillert Jan , Rubio Justin P , Abderrahim Hadi , Wojcik Jerome , Comi Giancarlo 
Published: March 2012

Background: The role played by genetic factors in influencing the clinical course of multiple sclerosis (MS) is not yet well established. Objective: We aimed to identify genetic variants associated with progressive MS (PrMS). Methods: We conducted a genome-wide association study (GWAS) in 197 patients with PrMS and 234 controls of Italian origin. We tested the top 20 single nucleotide polymorphisms (SNPs) with suggestive evidence of association ( p-value<10−4) in two independent sets of primary progressive MS cases and controls. Results: We identified a risk-associated SNP in the HLA region in linkage disequilibrium (LD) with DRB1*1501 and DQB*0602 loci, with genome-wide significance (rs3129934T, pcombined=6.7×10-16, OR=2.34, 95% CI=1.90–2.87), and a novel locus on chromosome 7q35 with suggestive evidence of association (rs996343G, pcombined=2.4×10-5, OR=0.70, 95% CI=0.59–0.83) which maps within a human endogenous retroviral (HERV) element. The new locus did not have a ‘ cis’ effect on RNA expression in lymphoblastic cell lines, but pathway analyses of ‘ trans’ effects point to an expression regulation of genes involved in neurodegeneration, including glutamate metabolism ( p<0.01) and axonal guidance signalling ( p<0.02). Conclusions: We have confirmed the established association with the HLA region and, despite the low statistical power of the study, we found suggestive evidence for association with a novel locus on chromosome 7, with a putative regulatory role.
Metaphoric language and the articulation of emotions by people affected by motor neurone disease
Authors: Locock Louise , Mazanderani Fadhila , Powell John 
Published: March 2012

Objective: To explore the use of metaphoric language to convey emotion in interviews with people affected by motor neurone disease, a progressive neurological condition that sits between chronic and terminal illness. Methods: Secondary analysis of 46 interviews with people affected by motor neurone disease in the United Kingdom (35 individuals with the condition, 11 carers). Results: Metaphor and figurative language was used to communicate the intensely emotional experiences of being diagnosed with and living with motor neurone disease. We focus on three pervasive themes that were threaded throughout the interviews: battling and fighting; the self under attack and journeying through a physical and emotional landscape. Discussion: This secondary analysis of qualitative research interviews enriches our understanding of the articulation of emotion in motor neurone disease and adds to the literature on metaphor in chronic illness. Of particular interest is how the metaphors used contrasted with other conditions in the relative absence of metaphors of ‘fighting’ the disease. Furthermore, we analyse the ways in which participants used metaphors to give voice to emotions that are extremely difficult to articulate in ‘literal’ language, and how, in doing so, they blurred the distinction between ‘physical’ symptoms and ‘emotional’ states. Sensitivity to metaphors may help professionals communicate with people affected by motor neurone disease.
Multiple myeloma exhibits novel dependence on GLUT4, GLUT8, and GLUT11: implications for glucose transporter-directed therapy
Authors: McBrayer Samuel K. , Cheng Javelin C. , Singhal Seema , Krett Nancy L. , Rosen Steven T. , Shanmugam Mala 
Published: March 2012

AbstractMultiple myeloma is one of numerous malignancies characterized by increased glucose consumption, a phenomenon with significant prognostic implications in this disease. Few studies have focused on elucidating the molecular underpinnings of glucose transporter (GLUT) activation in cancer, knowledge that could facilitate identification of promising therapeutic targets. To address this issue, we performed gene expression profiling studies involving myeloma cell lines and primary cells as well as normal lymphocytes to uncover deregulated GLUT family members in myeloma. Our data demonstrate that myeloma cells exhibit reliance on constitutively cell surface-localized GLUT4 for basal glucose consumption, maintenance of Mcl-1 expression, growth, and survival. We also establish that the activities of the enigmatic transporters GLUT8 and GLUT11 are required for proliferation and viability in myeloma, albeit because of functionalities probably distinct from whole-cell glucose supply. As proof of principle regarding the therapeutic potential of GLUT-targeted compounds, we include evidence of the antimyeloma effects elicited against both cell lines and primary cells by the FDA-approved HIV protease inhibitor ritonavir, which exerts a selective off-target inhibitory effect on GLUT4. Our work reveals critical roles for novel GLUT family members and highlights a therapeutic strategy entailing selective GLUT inhibition to specifically target aberrant glucose metabolism in cancer.
Rational design and characterization of platelet factor 4 antagonists for the study of heparin-induced thrombocytopenia
Authors: Sachais Bruce S. , Rux Ann H. , Cines Douglas B. , Yarovoi Serge V. , Garner Lee I. , Watson Stephen P. , Hinds Jillian L. , Rux John J. 
Published: March 2012

AbstractPatients with heparin-induced thrombocytopenia (HIT) remain at risk for recurrent thromboembolic complications despite improvements in management. HIT is caused by antibodies that preferentially recognize ultralarge complexes (ULCs) of heparin and platelet factor 4 (PF4) tetramers. We demonstrated previously that a variant PF4K50E forms dimers but does not tetramerize or form ULCs. Here, we identified small molecules predicted to bind PF4 near the dimer-dimer interface and that interfere with PF4 tetramerization. Screening a library of small molecules in silico for binding at this site, we identified 4 compounds that inhibited tetramerization at micromolar concentrations, designated PF4 antagonists (PF4As). PF4As also inhibited formation of pathogenic ULCs, and 3 of these PF4As promoted the breakdown of preformed ULCs. To characterize the ability of PF4As to inhibit cellular activation, we developed a robust and reproducible assay that measures cellular activation by HIT antibodies via FcγRIIA using DT40 cells. PF4As inhibit FcγRIIA-dependent activation of DT40 cells by HIT antibodies as well as platelet activation, as measured by serotonin release. PF4As provide new tools to probe the pathophysiology of HIT. They also may provide insight into the development of novel, disease-specific therapeutics for the treatment of thromboembolic complications in HIT.
Strong convergence theorems by Halpern-Mann iterations for multi-valued relatively nonexpansive mappings in Banach spaces with applications
Authors: Zhu Jin-hua , Chang Shih-sen , Liu Min 
Published: March 2012

Abstract
In this article, an iterative sequence for relatively nonexpansive multi-valued mapping by modifying Halpern and Mann's iterations is introduced, and then some strong convergence theorems are proved. At the end of the article some applications are given also.

AMS Subject Classification: 47H09; 47H10; 49J25.
Identification of microRNAs from Amur grape (vitis amurensis Rupr.) by deep sequencing and analysis of microRNA variations with bioinformatics
Authors: Wang Chen , Han Jian , Liu Chonghuai , Kibet Korir Nicholas , Kayesh Emrul , Shangguan Lingfei , Li Xiaoying , Fang Jinggui 
Published: March 2012

Abstract

Background
MicroRNA (miRNA) is a class of functional non-coding small RNA with 19-25 nucleotides in length while Amur grape (Vitis amurensis Rupr.) is an important wild fruit crop with the strongest cold resistance among the Vitis species, is used as an excellent breeding parent for grapevine, and has elicited growing interest in wine production. To date, there is a relatively large number of grapevine miRNAs (vv-miRNAs) from cultivated grapevine varieties such as Vitis vinifera L. and hybrids of V. vinifera and V. labrusca, but there is no report on miRNAs from Vitis amurensis Rupr, a wild grapevine species.

Results
A small RNA library from Amur grape was constructed and Solexa technology used to perform deep sequencing of the library followed by subsequent bioinformatics analysis to identify new miRNAs. In total, 126 conserved miRNAs belonging to 27 miRNA families were identified, and 34 known but non-conserved miRNAs were also found. Significantly, 72 new potential Amur grape-specific miRNAs were discovered. The sequences of these new potential va-miRNAs were further validated through miR-RACE, and accumulation of 18 new va-miRNAs in seven tissues of grapevines confirmed by real time RT-PCR (qRT-PCR) analysis. The expression levels of va-miRNAs in flowers and berries were found to be basically consistent in identity to those from deep sequenced sRNAs libraries of combined corresponding tissues. We also describe the conservation and variation of va-miRNAs using miR-SNPs and miR-LDs during plant evolution based on comparison of orthologous sequences, and further reveal that the number and sites of miR-SNP in diverse miRNA families exhibit distinct divergence. Finally, 346 target genes for the new miRNAs were predicted and they include a number of Amur grape stress tolerance genes and many genes regulating anthocyanin synthesis and sugar metabolism.

Conclusions
Deep sequencing of short RNAs from Amur grape flowers and berries identified 72 new potential miRNAs and 34 known but non-conserved miRNAs, indicating that specific miRNAs exist in Amur grape. These results show that a number of regulatory miRNAs exist in Amur grape and play an important role in Amur grape growth, development, and response to abiotic or biotic stress.
Epigenetic features are significantly associated with alternative splicing
Authors: Zhou Yuanpeng , Lu Yulan , Tian Weidong 
Published: March 2012

Abstract

Background
While alternative splicing (AS) contributes greatly to protein diversities, the relationship between various types of AS and epigenetic factors remains largely unknown.

Results
In this study, we discover that a number of epigenetic features, including DNA methylation, nucleosome occupancy, specific histone modifications and protein features, are strongly associated with AS. To further enhance our understanding of the association between these features and AS, we cluster our investigated features based on their association patterns with each AS type into four groups, with H3K36me3, EGR1, GABP, SRF, SIN3A and RNA Pol II grouped together and showing strongest association with AS. In addition, we find that the AS types can be classified into two general classes, namely the exon skipping related process (ESRP), and the alternative splice site selection process (ASSP), based on their association levels with the epigenetic features.

Conclusion
Our analysis thus suggests that epigenetic features are likely to play important roles in regulating AS.