bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dr. Astrov: Chekov's Notable Idealist
Authors: Polakiewicz Leonard A. 
Published: May 2011

AbstractChekhov depicted a number of idealists – individuals whose behavior and thought is based on ideals that for the most part prove to be impractical or unattainable in reality. These noble visionaries have a conception of things as they wish them to be. Many of Chekhov's idealists are doctors who, more often than not, suffer defeat on a personal rather than on a professional level. Dr. Astrov appears to suffer a defeat both on a personal level and in some respects on a professional level. The present study aims to provide an analysis of the nature of Dr. Astrov's idealism, his struggle with existential obstacles, and his personal and professional struggle against the complex forces that challenge his idealism and eventually turn him into a cynic.
Coherent Region-, Species-, and Frequency-Dependent Local Climate Signals in Northern Hemisphere Tree-Ring Widths*
Authors: Wettstein Justin J. , Littell Jeremy S. , Wallace John M. , Gedalof Ze’ev 
Published: May 2011

Abstract
Patterns of correlation between tree rings and local temperature or precipitation are investigated using 762 International Tree-Ring Data Bank standardized ring width site chronology time series, and a gridded dataset of temperature and precipitation. Coherent regional- and, in some cases, hemispheric-scale patterns of correlation are found in the extratropical Northern Hemisphere for both the summer prior to and the summer concurrent with ring width formation across different species and over large distances.
Among those chronologies that are primarily linked to temperature, thicker ring widths are generally associated with anomalously cool prior summer temperature and anomalously warm concurrent summer temperature. Reconstructions of local summer temperature using prior, concurrent, and/or subsequent year ring widths as predictors demonstrate that useful climate–growth information generally exists in ring widths that are both concurrent with and subsequent to the summer temperature anomaly. Consistent prior summer temperature–ring width relationships have received relatively little previous attention. Among those chronologies that are primarily linked to precipitation, thicker ring widths are generally associated with high summer precipitation in both the year prior to and the year concurrent with ring formation. The magnitude and spatial consistency of temperature correlations are greater than those for precipitation, at least on the hemispheric scale. These results support and serve to generalize the conclusions of prior regionally restricted and/or species-specific studies relating ring width to energy and/or water limitations.
Regional- and hemispheric-scale patterns of ring width–temperature or ring width–precipitation correlations show up more clearly in species-specific and frequency-dependent analyses. Different species respond differently to temperature and precipitation anomalies. Consistent with the hemispheric patterns described above, most standardized ring width time series more faithfully record the high frequency component of the temperature signal than the low frequency component. The potential for enhanced coherence in regionally restricted, species-specific, and frequency-dependent analyses is independently verified by examining the correlation between ring width time series over geographical distance. This broader characterization of relationships between tree-ring widths and local climate provides an objective basis for selecting tree ring or other similarly high-resolution proxy data for regional-, hemispheric-, or global-scale paleoclimate reconstructions.
Convective Storm Structures and Ambient Conditions Associated with Severe Weather over the Northeast United States
Authors: Lombardo Kelly A. , Colle Brian A. 
Published: May 2011

Abstract
This study documents the convective storm structures and ambient conditions associated with severe storms (wind, hail, and tornado) over the northeastern United States for two warm seasons (May–August), including 2007 and a warm season comprising randomly selected days from 2002 to 2006. The storms were classified into three main convective organizational structures (cellular, linear, and nonlinear) as well as several subcategories. The same procedure was applied to the highly populated coastal zone of the northeastern United States, including New Jersey, Connecticut, Rhode Island, and New York. The coastal analysis included six warm seasons from 2002 to 2007. Over the Northeast, severe wind events are evenly distributed among the cellular, linear, and nonlinear structures. Cellular structures are the primary hail producers, while tornadoes develop mainly from cellular and linear structures. Over the coastal zone, primarily cellular and linear systems produce severe wind and hail, while tornadoes are equally likely from all three convective structures. Composites were generated for severe weather days over the coastal region for the three main convective structures. On average, severe cellular events develop during moderate instability [most unstable CAPE (MUCAPE) ~1200 J kg−1], with low-level warm-air advection and frontogenesis at the leading edge of a thermal ridge collocated with an Appalachian lee trough. Severe linear events develop in a similar mean environment as the cellular events, except that most linear events occur with a surface trough upstream over the Ohio River valley and half of the linear events develop just ahead of progressive midlevel troughs. Nonlinear severe events develop with relatively weak mean convective instability (MUCAPE ~460 J kg−1), but they are supported by midlevel quasigeostrophic (QG) forcing for ascent.
Morning headache in habitual snorers: Frequency, characteristics, predictors and impacts
Authors: Chen Ping-Kun , Fuh Jong-Ling , Lane Hsien-Yuan , Chiu Pai-Yi , Tien Hui-Chi , Wang Shuu-Jiun 
Published: May 2011

Objectives: Morning headache has been considered as an accompanying symptom of obstructive sleep apnoea syndrome (OSAS). However the frequency, characteristics, predictors and impacts of morning headache in habitual snorers are not well defined. Methods: We studied consecutive patients with habitual snoring in a sleep laboratory using polysomnography. All patients were interviewed by a physician regarding the presence or absence of morning headache, migraine and insomnia. Each patient completed the Short Form-36 health survey (SF-36) and the Hospital Anxiety and Depression Scale (HADS). Morning headache was defined as headache on awakening ≥1 day/week for ≥6 months. Results: Of the 268 participants with habitual snoring, 63 (23.5%) had morning headache and 184 (69%) had OSAS. Patients with morning headache reported lower scores in all eight domains of the SF-36 than those without (difference: 10.6 to 29.7 points, all p ≤ 0.005). The independent predictors of morning headache were migraine (adjusted odds ratio (AOR) 6.3), insomnia (AOR 4.2), psychological distress (HADS ≥ 8) (AOR 3.9) and OSAS (AOR 2.6). Morning headache in 12 patients (19%) fulfilled the criteria for migraine attacks. Conclusions: Morning headache was common in habitual snorers and associated with a pervasive impairment of health-related quality of life. Migrainous features were not uncommon. Not only OSAS, but migraine, insomnia and psychological distress were also important predictors for morning headache, even in snoring patients.
Abrogation of donor T-cell IL-21 signaling leads to tissue-specific modulation of immunity and separation of GVHD from GVL
Authors: Hanash Alan M. , Kappel Lucy W. , Yim Nury L. , Nejat Rebecca A. , Goldberg Gabrielle L. , Smith Odette M. , Rao Uttam K. , Dykstra Lindsay , Na Il-Kang , Holland Amanda M. , Dudakov Jarrod A. , Liu Chen , Murphy George F. , Leonard Warren J. , Heller Glenn , van den Brink Marcel R. M. 
Published: May 2011

AbstractIL-21 is a proinflammatory cytokine produced by Th17 cells. Abrogation of IL-21 signaling has recently been shown to reduce GVHD while retaining graft-versus-leukemia/lymphoma (GVL) responses. However, the mechanisms by which IL-21 may lead to a separation of GVHD and GVL remain incompletely understood. In a murine MHC-mismatched BM transplantation model, we observed that IL-21 receptor knockout (IL-21R KO) donor T cells mediate decreased systemic and gastrointestinal GVHD in recipients of a transplant. This reduction in GVHD was associated with expansion of transplanted donor regulatory T cells and with tissue-specific modulation of Th-cell function. IL-21R KO and wild-type donor T cells showed equivalent alloactivation, but IL-21R KO T cells showed decreased infiltration and inflammatory cytokine production within the mesenteric lymph nodes. However, Th-cell cytokine production was maintained peripherally, and IL-21R KO T cells mediated equivalent immunity against A20 and P815 hematopoietic tumors. In summary, abrogation of IL-21 signaling in donor T cells leads to tissue-specific modulation of immunity, such that gastrointestinal GVHD is reduced, but peripheral T-cell function and GVL capacity are retained. IL-21 is thus an exciting target for therapeutic intervention and improvement of clinical transplantation outcomes.
Sumoylation of CCAAT/enhancer–binding protein α promotes the biased primitive hematopoiesis of zebrafish
Authors: Yuan Hao , Zhou Jun , Deng Min , Zhang Yong , Chen Yi , Jin Yi , Zhu Jiang , Chen Sai Juan , de The Hugues , Chen Zhu , Liu Ting Xi , Zhu Jun 
Published: May 2011

AbstractHematopoiesis is evolutionarily conserved from zebrafish to mammals, and this includes both primitive and definitive waves during embryogenesis. Primitive hematopoiesis is dominated by erythropoiesis with limited myelopoiesis. Protein sumoylation, a ubiquitination-like posttranslational protein modification, is implicated in a variety of biochemical processes, most notably in transcriptional repression. We show here that the loss of 6 small ubiquitin-related modifier (SUMO) paralogs triggers a sharp up-regulation of the myeloid-specific marker mpo and down-regulation of the erythroid-specific marker gata1 in myelo-erythroid progenitor cells (MPCs) in the intermediate cell mass (ICM) during primitive hematopoiesis. Accordingly, in transgenic zebrafish lines, hyposumoylation expands myelopoiesis at the expense of erythropoiesis. A SUMO–CCAAT/enhancer–binding protein α (SUMO-C/ebpα) fusion restores the normal myelopoiesis/erythropoiesis balance, suggesting that sumoylation status of C/ebpα contributes to myelo-erythroid lineage determination. Our results therefore implicate sumoylation in early lineage determination and reveal the possible molecular mechanism underlying the puzzling biased primitive hematopoiesis in vertebrates.
Atypical epigenetic mark in an atypical location: cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene
Authors: González Rodrigo M , Ricardi Martiniano M , Iusem Norberto D 
Published: May 2011

Abstract

Background
Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant DNA methylation, which is stimulated by external cues exemplified by various abiotic types of stress, is often found not only at CG sites but also at CNG (N denoting A, C or T) and CNN (asymmetric) sites. A genome-wide analysis of DNA methylation in Arabidopsis has shown that CNN methylation is preferentially concentrated in transposon genes and non-coding repetitive elements. We are particularly interested in investigating the epigenetics of plant species with larger and more complex genomes than Arabidopsis, particularly with regards to the associated alterations elicited by abiotic stress.

Results
We describe the existence of CNN-methylated epialleles that span Asr1, a non-transposon, protein-coding gene from tomato plants that lacks an orthologous counterpart in Arabidopsis. In addition, to test the hypothesis of a link between epigenetics modifications and the adaptation of crop plants to abiotic stress, we exhaustively explored the cytosine methylation status in leaf Asr1 DNA, a model gene in our system, resulting from water-deficit stress conditions imposed on tomato plants. We found that drought conditions brought about removal of methyl marks at approximately 75 of the 110 asymmetric (CNN) sites analysed, concomitantly with a decrease of the repressive H3K27me3 epigenetic mark and a large induction of expression at the RNA level. When pinpointing those sites, we observed that demethylation occurred mostly in the intronic region.

Conclusions
These results demonstrate a novel genomic distribution of CNN methylation, namely in the transcribed region of a protein-coding, non-repetitive gene, and the changes in those epigenetic marks that are caused by water stress. These findings may represent a general mechanism for the acquisition of new epialleles in somatic cells, which are pivotal for regulating gene expression in plants.
Small invasive colon cancer with systemic metastasis: A case report
Authors: Matsumoto Minori , Nakajima Takeshi , Kato Ken , Kouno Tsutomu , Sakamoto Taku , Matsuda Takahisa , Kushima Ryoji , Saito Yutaka 
Published: May 2011

ABSTRACT

Background
Recently, especially in Japan, several researchers have suggested that colorectal cancer can develop not only through an adenoma-carcinoma sequence but also from normal mucosa via a de novo pathway, and that these de novo cancers have more aggressive malignant potential. We report a case of aggressive colon cancer resulting in systemic metastasis despite small tumour size.

Case Presentation
A 35-year-old woman presented at the referring hospital with swelling of the left cervical lymph node. Biopsy of the lymph node revealed metastatic adenocarcinoma; however, CT scan and mammography were unable to identify the site of the primary lesion. She was diagnosed with unknown primary cancer and referred to our hospital for further examination. Immunohistochemical reevaluation showed the cervical lymph node biopsy specimen to be positive for CDX2 and CK20 and negative for CK7 expression, leading us to suspect the presence of a primary colorectal cancer. We performed a total colonoscopy, and detected a small protruding lesion in the transverse colon. The tumour was only 12 mm in diameter, with a central depressed component and a severely thickened stalk, which suggested direct cancer invasion of the deep submucosa. We concluded that this lesion was the site of origin of the metastasis despite the small tumour size, and performed diagnostic endoscopic mucosal resection. The lesion was found to have an intramucosal cancer component, demonstrating that this lesion represented primary colon cancer. The patient was referred to the gastrointestinal oncology division for systemic chemotherapy.

Conclusions
In this case, immunohistochemical findings strongly suggested the existence of a colorectal cancer. The non-polypoid gross appearance of the tumour suggested that it can originate de novo , thus providing a valuable case in support of the aggressive malignant potential of a de novo colorectal cancer pathway.
Understanding, comprehensibility and acceptance of an evidence-based consumer information brochure on fall prevention in old age: a focus group study
Authors: Lins Sabine , Icks Andrea , Meyer Gabriele 
Published: May 2011

Abstract

Background
Evidence-based patient and consumer information (EBPI) is an indispensable component of the patients' decision making process in health care. Prevention of accidental falls in the elderly has gained a lot of public interest during preceding years. Several consumer information brochures on fall prevention have been published; however, none fulfilled the criteria of an EBPI. Little is known about the reception of EBPI by seniors. Therefore we aimed to evaluate a recently developed EBPI brochure on fall prevention with regard to seniors' acceptance and comprehensibility in focus groups and to explore whether the participants' judgements differed depending on the educational background of the study participants.

Methods
Seven focus groups were conducted with 40 seniors, aged 60 years or older living independently in a community. Participants were recruited by two gatekeepers. A discussion guide was used and seniors were asked to judge the EBPI brochure on fall prevention using a Likert scale 1-6. The focus group discussions were tape recorded, transcribed verbatim, and analysed using content analysis.

Results
The participants generally accepted the EBPI brochure on fall prevention. Several participants expressed a need for more practical advice. The comprehensibility of the brochure was influenced positively by brief chapter summaries. Participants dismissed the statistical illustrations such as confidence intervals or a Fagan nomogram and only half of them agreed with the meta-information presented in the first chapter. The detailed information about fall prevalence was criticised by some seniors. The use of a case story was well tolerated by the majority of participants.

Conclusion
Our findings indicate that the recently developed EBPI brochure on fall prevention in old age was generally well accepted by seniors, but some statistical descriptions were difficult for them to understand. The brochure has to be updated. However, not all issues raised by the participants will be taken into account since some of them are contrary to the principles of EBPI.
Monosodium Iodoacetate-Induced Joint Pain is Associated with Increased Phosphorylation of Mitogen Activated Protein Kinases in the Rat Spinal Cord
Authors: Lee Younglim , Pai Madhavi , Brederson Jill-Desiree , Wilcox Denise , Hsieh Gin , Jarvis Michael F , Bitner Robert S 
Published: May 2011

Background: Intra-articular injection of monosodium iodoacetate (MIA) in the knee joint of rats disrupts chondrocyte metabolism resulting in cartilage degeneration and subsequent nociceptive behavior that has been described as a model of osteoarthritis (OA) pain. Central sensitization through activation of mitogen activated protein kinases (MAPKs) is recognized as a pathogenic mechanism in chronic pain. In the present studies, induction of central sensitization as indicated by spinal dorsal horn MAPK activation, specifically ERK and p38 phosphorylation, was assessed in the MIA-OA model. Results: Behaviorally, MIA-injected rats displayed reduced hind limb grip force 1, 2, and 3 weeks post-MIA treatment. In the same animals, activation of phospho ERK1/2 was gradually increased, reaching a significant level at post injection week 3. Conversely, phosphorylation of p38 MAPK was enhanced maximally at post injection week 1 and decreased, but remained elevated, thereafter. Double labeling from 3-wk MIA rats demonstrated spinal pERK1/2 expression in neurons, but not glia. In contrast, p-p38 was expressed by microglia and a subpopulation of neurons, but not astrocytes. Additionally, there was increased ipsilateral expression of microglia, but not astrocytes, in 3-wk MIA-OA rats. Consistent with increased MAPK immunoreactivity in the contralateral dorsal horn, mechanical allodynia to the contralateral hind-limb was observed 3-wk following MIA. Finally, intrathecal injection of the MEK1 inhibitor PD98059 blocked both reduced hind-limb grip force and pERK1/2 induction in MIA-OA rats. Conclusion: Results of these studies support the role of MAPK activation in the progression and maintenance of central sensitization in the MIA-OA experimental pain model.
Association of the rs3743205 variant of DYX1C1 with dyslexia in Chinese children
Authors: Lim Cadmon K P , Ho Connie S H , Chou Crystal H N , Waye Mary M Y 
Published: May 2011

Abstract

Background
Dyslexia is a learning disability that is characterized by difficulties in the acquisition of reading and spelling skills independent of intelligence, motivation or schooling. Studies of western populations have suggested that DYX1C1 is a candidate gene for dyslexia. In view of the different languages used in Caucasian and Chinese populations, it is therefore worthwhile to investigate whether there is an association of DYX1C1 in Chinese children with dyslexia.

Method and Results
Eight single nucleotide polymorphisms (SNPs) were genotyped from three hundred and ninety three individuals from 131 Chinese families with two which have been reported in the literature and six tag SNPs at DYX1C1. Analysis for allelic and haplotypic associations was performed with the UNPHASED program and multiple testing was corrected using false discovery rates. We replicated the previously reported association of rs3743205 in Chinese children with dyslexia (p

corrected
= 0.0072). This SNP was also associated with rapid naming, phonological memory and orthographic skills in quantitative trait analysis.

Conclusion
Our findings suggest that DYX1C1 is associated with dyslexia in people of Chinese ethnicity in Hong Kong.
ConReg-R: Extrapolative recalibration of the empirical distribution of p-values to improve false discovery rate estimates
Authors: Li Juntao , Paramita Puteri , Choi Kwok Pui , Karuturi R Krishna Murthy 
Published: May 2011

Abstract

Background
False discovery rate (FDR) control is commonly accepted as the most appropriate error control in multiple hypothesis testing problems. The accuracy of FDR estimation depends on the accuracy of the estimation of p-values from each test and validity of the underlying assumptions of the distribution. However, in many practical testing problems such as in genomics, the p-values could be under-estimated or over-estimated for many known or unknown reasons. Consequently, FDR estimation would then be influenced and lose its veracity.

Results
We propose a new extrapolative method called Constrained Regression Recalibration (ConReg-R) to recalibrate the empirical p-values by modeling their distribution to improve the FDR estimates. Our ConReg-R method is based on the observation that accurately estimated p-values from true null hypotheses follow uniform distribution and the observed distribution of p-values is indeed a mixture of distributions of p-values from true null hypotheses and true alternative hypotheses. Hence, ConReg-R recalibrates the observed p-values so that they exhibit the properties of an ideal empirical p-value distribution. The proportion of true null hypotheses (π
0) and FDR are estimated after the recalibration.

Conclusions
ConReg-R provides an efficient way to improve the FDR estimates. It only requires the p-values from the tests and avoids permutation of the original test data. We demonstrate that the proposed method significantly improves FDR estimation on several gene expression datasets obtained from microarray and RNA-seq experiments.

Reviewers
The manuscript was reviewed by Prof. Vladimir Kuznetsov, Prof. Philippe Broet, and Prof. Hongfang Liu (nominated by Prof. Yuriy Gusev).