bluuebunny/crossref_metadata_2025_split huggingface dataset:
Azathioprine/hydroxyurea preconditioning prior to nonmyeloablative matched sibling donor hematopoietic stem cell transplantation in adults with sickle cell disease: A prospective observational cohort study
Authors: Dovern Elisabeth , Aydin Mesire , Hazenberg Mette D. , Tang Man Wai , Suijk Elisabeth M. , Hoogendoorn Gerianne M. , Van Tuijn Charlotte F. J. , Kerkhoffs Jean‐Louis , Rutten Caroline E. , Zeerleder Sacha S. , de la Fuente Josu , Biemond Bart J. , Nur Erfan 
Published: May 2024

AbstractNonmyeloablative, matched sibling donor hematopoietic stem cell transplantation with alemtuzumab/total body irradiation (TBI) conditioning is a curative therapy with low toxicity for adults with sickle cell disease (SCD). However, relatively low donor chimerism levels and graft rejection remain important challenges. We hypothesized that adding azathioprine/hydroxyurea preconditioning will improve donor chimerism levels and reduce graft failure rate. In this prospective cohort study, we enrolled consecutive adult patients with SCD undergoing matched sibling donor transplantation at the Amsterdam UMC. Patients received azathioprine 150 mg/day and hydroxyurea 25 mg/kg/day for 3 months prior to alemtuzumab 1 mg/kg and 300 cGy TBI conditioning. Twenty patients with SCD (median age 26 years [range 19–49], 13 females) were transplanted. Median follow‐up was 46.0 months (IQR 21.8–57.9). One‐year overall survival and event‐free survival (graft failure or death) were both 95% (95% confidence interval 86–100). Mean donor myeloid and T‐cell chimerism 1‐year post‐transplant were 95.2% (SD ±10.6) and 67.3% (±15.3), respectively. One patient (5%) experienced graft failure without autologous regeneration, resulting in infections and death. All other patients had a corrected SCD phenotype and were able to discontinue sirolimus. Three patients were successfully treated with alemtuzumab (1 mg/kg) after the transplant because of declining donor chimerism and cytopenias to revert impending graft rejection. Toxicity was mostly related to sirolimus and alemtuzumab. One patient developed steroid‐responsive grade II intestinal acute graft‐versus‐host disease. Collectively, preconditioning with azathioprine/hydroxyurea prior to nonmyeloablative matched sibling donor transplantation resulted in excellent event‐free survival and robust donor T‐cell chimerism, enabling the successful withdrawal of sirolimus. ClinicalTrials.gov: NCT05249452.
Selective Hydrogenation of Unsaturated Vinyl Ethers in Two‐Chamber Gauge‐Reactor
Authors: Gyrdymova Yulia V. , Saybulina Elina R. , Mironenko Roman M. , Rodygin Konstantin S. 
Published: May 2024

AbstractA pressure gauge was incorporated into a two‐chamber glass H‐tube to continuously monitor the pressure inside a reactor when working with gases. The ex situ generated H2, D2, acetylene and CO2 were tested in pressure gauge reactors, and calibration curves were plotted. A series of unsaturated compounds with two or more double C=C bonds accessible for hydrogenation were synthesized for hydrogenation. The hydrogenation proceeded well due to constant pressure control, and the desired products were isolated in good yields. The same hydrogenation procedures were then carried out using steel autoclaves to verify the results and compare them. Surprisingly, almost the same results were obtained, confirming the effectiveness of self‐made glass reactors. The hydrogenation mechanism was further investigated using D‐labeled reagents. The kinetics of hydrogenation was studied both in manometric glass reactors and in steel autoclaves, which showed a similar nature of the main process in both reaction units. Thus, the created reactors can be effectively used in barometric transformations instead of steel autoclaves in appropriate cases.
Dumbbell‐Shaped, Block‐Graft Copolymer with Aligned Domains for High‐Performance Hydrocarbon Polymer Electrolyte Membranes
Authors: Lee So Youn , Kang Du Ru , Oh Jong‐Gil , Chae Il Seok , Kim Jong Hak 
Published: May 2024

AbstractGiven the environmental concerns surrounding fluoromaterials, the use of high‐cost perfluorinated sulfonic acids (PFSAs) in fuel cells and water electrolysis contradicts the pursuit of clean energy systems. Herein, we present a fluorine‐free dumbbell‐shaped block‐graft copolymer, derived from the cost‐effective triblock copolymer, poly(styrene‐b‐ethylene‐co‐butylene‐b‐styrene) (SEBS), for polymer electrolyte membranes (PEMs). This unique polymer shape led to the alignment of the hydrophobic‐hydrophilic domains along a preferred orientation, resulting in the construction of interconnected proton channels across the membrane. A bicontinuous network allowed efficient proton transport with reduced tortuosity, leading to an exceptional ionic conductivity (249 mS cm−1 at 80 °C and 90 % relative humidity (RH)), despite a low ion exchange capacity (IEC; 1.41). Furthermore, membrane electrode assembly (MEA) prepared with our membrane exhibited stable performance over a period of 150 h at 80 °C and 30 % RH. This study demonstrates a novel polymer structure design and highlights a promising outlook for hydrocarbon PEMs as alternatives to PFSAs.
In vitro analysis of electrospun nanofibrous polycaprolactone loaded with Rhamnus prinoides leaf extract as potential wound dressing
Authors: Adamu Biruk Fentahun , Gao Jing , Yuan Xiangnan , Tan Shaojie , Kumelachew Degu Melaku , Jhatial Abdul Khalique 
Published: May 2024

AbstractRhamnus prinoides, an indigenous Ethiopian traditional medicinal plant, has been used for treating various ailments including skin conditions in traditional formulations. In this study, plant leaf extract has been incorporated into polycaprolactone (PCL) nanofibers through electrospinning at concentrations of 1%, 3%, 5%, 7% wt/vol for the first time. The presence of extract in the nanofiber was analyzed using Fourier transform infrared. The in vitro antibacterial activity, release, contact angle, thermal, and mechanical properties of the nano mats were characterized. The scanning electron microscopy (SEM) analysis revealed that the produced mats are continuous, smooth, bead‐less, and inter connected. The examination of SEM images using ImageJ revealed that the fiber diameter ranged from 219 ± 47 nm to 284 ± 114 nm. The mats showed excellent anti‐bactericidal activity against both Staphylococcus aureus and Escherichia coli. During the 72‐h in vitro release investigation, an initial burst release was observed within the first 12 h, followed by sustained release of the extract over long period of time. The contact angle analysis showed, addition of leaf extract to the PCL nanofiber increased hydrophilicity. Furthermore, the nanofiber membranes had favorable mechanical properties. Overall, these findings suggested that R. prinoides leaf extract‐functionalized PCL nanofibers could be effective wound dressings with broad‐spectrum antimicrobial activity.
Design and development of shape memory polyurethane coated fabric with excellent memory performance
Authors: Garg Hema , Mohanty Jayashree , Das Apurba , Tripathi Bijay P. , Kumar Bipin 
Published: May 2024

AbstractIn this study, we investigate the fabrication of ambient room temperature responsive shape memory polyurethane (SMPU)‐coated fabric using a simple dip coating method, aiming to enhance the shape memory response of smart textiles. Two SMPU were synthesized using different chain extender 1,4 butanediol (BDO‐SMPU) and polyethylenimine (PEI‐SMPU). Critical parameters such as SMPU concentration, dwell time, and coating solution temperature are optimized to ensure exceptional shape memory performance while also maintaining the fabric's handle, comfort, and durability. We determined that PEI‐SMPU/Cotton fabric exhibits superior memory performance, with a 90.2% shape recovery at a 1% PEI‐SMPU concentration. Conversely, BDO‐SMPU fabric demonstrated a 75.8% shape recovery under different conditions (10% SMPU concentration, 50°C solution temperature). Furthermore, shape recovery is influenced by programming temperature and load; at 35°C and 10 N load, PEI‐SMPU/Cotton significantly outperforms BDO‐SMPU/Cotton, showing about 20% improvement in shape recovery. This study indicates that modifying SMPU from BDO‐SMPU to PEI‐SMPU can notably enhance shape recovery performance at 35°C, marking a significant advancement in human body temperature responsive smart fabric technology.
Preparation of surface molecularly‐imprinted magnetic kaolin for selective separation and determination of thiabendazole
Authors: Zengin Adem 
Published: May 2024

AbstractIn this study, a selective sorbent was fabricated by combining a surface molecularly‐imprinted polymer and magnetic kaolin for reliable quantification of thiabendazole (TBZ) coupled with spectrofluorimetry. Several surface characterization methods were conducted to confirm the synthesis of TBZ‐imprinted magnetic kaolin with a polymer thickness of ~48 nm. The molecularly‐imprinted magnetic kaolin (MIP@MKLN) showed high adsorption capacity (8.7 mg/g), remarkable selectivity with an imprinting factor of 3.71 and excellent regeneration ability for up to 10 uses without significant change of its initial adsorption capacity for TBZ. The proposed method had linear range from 5 to 500 μg/mL TBZ concentration with a correlation coefficient of 0.995 and the limit of detection was 1.4 μg/mL. MIP@MKLN was used for selective separation and quantification of TBZ in orange and lemon samples. There were high recovery values ranging from 96.4% to 100.2% with lower relative standard deviations (less than 5%) in the spiked samples under optimized conditions. Overall, the proposed method based on MIP@MKLN as an excellent sorbent could produce a feasible, convenient, and promising method for rapid, selective, and sensitive quantification of TBZ in real samples.
Integrating meta‐analysis of genome‐wide association study with Pig Genotype‐Tissue Expression resources uncovers the genetic architecture for age at first farrowing in pigs
Authors: Lin Qing , Feng Xueyan , Li Tingting , Pan Xiangchun , Diao Shuqi , Gao Yahui , Yuan Xiaolong , Li Jiaqi , Ding Xiangdong , Zhang Zhe 
Published: May 2024

AbstractAge at first farrowing (AFF) is a reproductive trait with low heritability and high importance in the pig industry. To enhance the statistical power of genome‐wide association study (GWAS) and further explore the genetic nature of AFF, we first conducted GWAS meta‐analysis using three Yorkshire populations, and then integrated the Pig Genotype‐Tissue Expression (PigGTEx) resources to interpret their potential regulatory mechanism. Additionally, we compared the AFF in pig with the age at first birth (AFB) of human using GWAS summary statistics. We detected 18 independent variants in GWAS meta‐analysis and 8 genes in gene‐based association analysis significantly associated with AFF. By integrating the PigGTEx resource, we conducted transcriptome‐wide association study (TWAS) and colocalization analysis on 34 pig tissues. In TWAS, we detected 18 significant gene‐tissue pairs, such as DCAF6 in uterus and CREG1 in blood. In colocalization, we found 111 potential candidate tissue‐gene pairs, such as GJD4 and LYPLAL1. We found that the homologous gene, CHST10, might be the potential candidate gene between humans in AFB and pigs in AFF. In conclusion, integrating GWAS meta‐analysis and PigGTEx resources is a meaningful way to decipher the genetic architecture of complex traits. We found that DCAF6, CREG1, GJD4, and LYPLAL1 are candidate genes with high reliability for AFF in swine. The comparative analysis showed that CHST10 might play a potentially critical role in AFB/AFF across human and pigs.
Unexplained massive pulmonary embolism in acromegaly patient: A case report
Authors: Al‐Anbagi Usamah , Usman Shybin , Saad Abdulrahman , Nashwan Abdulqadir J. 
Published: May 2024

Key Clinical MessageOur case highlights the importance of recognizing acromegaly as a potential risk factor for venous thromboembolism (VTE). Despite a thorough thrombophilia workup yielding unremarkable results, further research is warranted to elucidate the underlying mechanisms linking acromegaly and thrombophilia. This understanding will aid in improving risk assessment and management strategies for patients with acromegaly.AbstractAcromegaly, a rare disorder characterized by excessive growth hormone secretion, is associated with various comorbidities including hypertension, diabetes mellitus, and obstructive sleep apnea. While previous studies have identified abnormalities in hemostatic factors in acromegaly patients, the association between acromegaly and venous thromboembolism (VTE) remains poorly understood. We present the case of a 36‐year‐old male with a history of acromegaly who presented with acute dyspnea, chest pain, and cough. Despite a prior trans‐sphenoidal hypophysectomy, his acromegaly symptoms persisted. Upon evaluation, he was found to have bilateral pulmonary embolism. Thorough thrombophilia workup was unremarkable, suggesting acromegaly as a potential risk factor for VTE.
Modular Synthesis of [6,6]‐Oxaspirolactones Enabled by Palladium‐Catalyzed Carbonylative Spirolactonization
Authors: Alemayehu Asserese Ketema , Ding Yongzheng , Huang Hanmin 
Published: May 2024

AbstractAn expedient and efficient carbonylative protocol proceeded via palladium‐catalyzed tandem cyclic ketonization and carbonylative spirolactonization has been developed. This carbonylation reaction is compatible with a wide range of hydroxyalkyl ketone tethered benzyl chlorides bearing different functional groups and can be proceeded under comericially available Pd2(dba)3/BINAP catalyst system, providing a straithfowrd and practical approach to a broad range of [6,6]‐oxaspirolactones. The synthesized [6,6]‐oxaspirolactones are commonly found as core structures in natural products and can potentially serve as C2‐symmetric ligand synthons.
Alternative weighting schemes for fine‐tuned extended similarity indices
Authors: López Pérez Kenneth , Rácz Anita , Bajusz Dávid , Gonzalez Camila , Héberger Károly , Miranda‐Quintana Ramón Alain 
Published: May 2024

AbstractExtended similarity indices (i.e., generalization of pairwise similarity) have recently gained importance because of their simplicity, fast computation, and superiority in tasks like diversity picking. However, they operate with several meta parameters that should be optimized. Earlier, we extended the binary similarity indices to “discrete non‐binary” and “continuous” data; now we continue with introducing and comparing multiple weighting functions. As a case study, the similarity of CYP enzyme inhibitors (4016 molecules after curation) was characterized by their extended similarities, based on 2D descriptors, MACCS and Morgan fingerprints. A statistical workflow based on sum of ranking differences (SRD) and analysis of variance (ANOVA) was used for finding the optimal weight function(s). Overall, the best weighting function is the fraction (“frac”), which corresponds to the principle of parsimony. Optimal extended similarity indices were also found, and their differences are revealed across different data sets. We intend this work to be a guideline for users of extended similarity indices regarding the various weighting options available. Source code for the calculations is available at https://github.com/mqcomplab/MultipleComparisons.
An optimized cooperative data transmission scheme in relay‐enabled cellular communication networks
Authors: Wang Hanwu , Ren Feng , Zhang Lizhuo 
Published: May 2024

SummaryCooperative transmission is of great significance in data transmission and resource schedule in mobile cellular communication networks. To attain the desired network transmission performance objectives over the whole cellular network system, we propose an appropriate cooperative data transmission scheme to optimize the data delivery efficiency in terms of the transmission time consumption for each and every transmission group. The proposed transmission optimization scheme includes the tasks of determining the transmission group, allocating the channel rate resource, specifying the cooperative transmission mechanism, and performing resource allocation and schedule as well. Firstly, we propose a utility function based rate allocation scheme to optimize the channel rate resource utilization for all those transmission groups over the cellular communication network. Based on it, we further present a cooperative data transmission scheme to deal with the data delivery and reception for all those subgroups with high efficiency over the whole network system. Particularly, we propose a resource efficiency function‐based coordination scheduling (EFCS) scheme to conduct the cooperative data transmission and resource schedule as well for each transmission group within the network. The EFCS scheme facilitates to determine the subgroups for schedule over each scheduling cycle, which can produce the best data transmission efficiency across both the direct and cooperative transmission hop. Both the theoretical analysis and the simulation evaluation results verify the superior performance of our proposed cooperative data transmission scheme.
Application of optimization algorithm for virtual reference tag assisted localization and tracking of RFID
Authors: Mathavan Nagaraj , Siva Ranjani Seenivasan , Suresh Seetharaman , Bhuvanesh Ananthan 
Published: May 2024

SummaryIn an indoor setting, the radio frequency communication is utilized to locate and track mobile objects utilizing Radio Frequency Identification (RFID) technology. Measurements from the Received Signal Strength Indicator (RSSI) are typically the foundation of the localization technique. In an RFID‐based interior setting, lowering tracking mistakes and increasing the precision of tracking remain difficult tasks. In order to address these issues, we developed the VIRALTRACK (Virtual Reference Tag Localization and Tracking) framework that consists of four procedures: deep reinforced learning‐based tracking, quantum‐based localization, optimization‐based virtual reference tag allocation, and signal enhancement. In order to increase the signal's effectiveness, we initially suggested using the Extended Gradient Filter (EGF) technique to eliminate RSSI oscillations. In the second step, we suggested using the Emperor Penguin Colony (EPC) optimization technique to allocate the virtual reference tag while taking the number of tags, SNR, and temperature and humidity of the surroundings into account. In the third phase, we use a quantum neural network (QNN) for localization in order to estimate the position of the moving target. We introduced the SignRank approach to select the best virtual reference tag for localization, which lowers tracking mistakes. In conclusion, we presented the Twin Delayed Deep Deterministic Policy Gradient (TD3) method that boosts the tracking precision by tracking the moving target tag efficiently and taking into account stage, the orientation, distance, and valuable coordinates. The NS3.26 network simulator is used to run the simulation, and tracking precision, tracking error, and accumulated probabilities are used to assess effectiveness.
World Health Organization Reporting System for Soft Tissue Cytopathology: Risk of malignancy and reproducibility of categories among observers
Authors: Layfield Lester J. , Dodd Leslie , Esebua Magda 
Published: May 2024

AbstractIntroductionIn 2024, the World Health Organization (WHO) is scheduled to publish the WHO Reporting System for Soft Tissue Cytopathology (WHORSSTC). This system establishes categories with well‐defined definitions, criteria, and estimated risks of malignancy (ROMs) for soft tissue tumors. The estimates of ROM are based on a relatively small number of published studies. Interobserver reproducibility is not addressed in the reporting system even though reproducibility of a reporting system is highly important.MethodsA manual search of one authors personal consultation files and teaching set (L.J.L.) was conducted for all cytologic specimens of soft tissue tumors accessioned between January 1, 1985 and December 31, 2022. Only cases with documented surgical pathology follow‐up were included in the study. Slides from each case were evaluated independently by three cytopathologists with each case assigned to one of the WHORSSTC categories. A ROM for each of the WHORSSTC categories was calculated. Interobserver agreement was evaluated by the kappa and weighted kappa statistics.ResultsRisk for malignancy by category were: Category 1: 0%, Category 2: 28%, Category 3: 57%, Category 4: 47%, Category 5: 63%, and Category 6: 88%. Kappa statistics for agreement between raters varied from 0.2183 to 0.3465 and weighted kappa varied from 0.3778 to 0.5217.ConclusionsThe WHORSSTC showed a progression of malignancy risk from the category “benign” (28%) to the category “malignant” (88%). Interobserver agreement was only fair.
Development of node architecture and emergence of molecular organizer characteristics in the pig embryo
Authors: Harmoush Braah , Viebahn Christoph , Tsikolia Nikoloz 
Published: May 2024

AbstractBackgroundThe avian node is the equivalent of the amphibian Spemann's organizer, as indicated by its ability to induce a secondary axis, cellular contribution, and gene expression, whereas the node of the mouse, which displays limited inductive capacities, was suggested to be a part of spatially distributed signaling. Furthermore, the structural identity of the mouse node is subject of controversy, while little is known about equivalent structures in other mammals.ResultsWe analyzed the node and emerging organizer in the pig using morphology and the expression of selected organizer genes prior to and during gastrulation. The node was defined according to the “four‐quarter model” based on comparative consideration. The node of the pig displays a multilayered, dense structure that includes columnar epithelium, bottle‐like cells in the dorsal part, and mesenchymal cells ventrally. Expression of goosecoid (gsc), chordin, and brachyury, together with morphology, reveal the consecutive emergence of three distinct domains: the gastrulation precursor domain, the presumptive node, and the mature node. Additionally, gsc displays a ventral expression domain prior to epiblast epithelialization.ConclusionOur study defines the morphological and molecular context of the emerging organizer equivalent in the pig and suggests a sequential development of its function.