bluuebunny/crossref_metadata_2025_split huggingface dataset:
RET
Alterations Differentiate Molecular Profile of Medullary Thyroid Cancer
Authors: Prabhash Kumar , Saldanha Elveera , Patil Vijay , Bal Munita , Reddy P Sreekanth , Sanjeev Airy , Kumar Raunak , Poojary Disha , Noronha Vanita , Menon Nandini , Mittal Neha , Trivedi Vaishakhi , Nambiar Kavya , Mishra Rohit , Tanwar Nishtha , Malhotra Richa , Pange Priyanka , Gupta Vinod , Veldore Vidya H. , Chougule Anuradha , Chaturvedi Pankaj , Dutt Amit , Chandrani Pratik 
Published: May 2024

PURPOSE

Medullary thyroid cancer (MTC) is a rare cancer originating from parafollicular C cells of the thyroid gland. Therapeutically relevant alterations in MTC are predominantly reported in
RET
oncogene, and lower-frequency alterations are reported in
KRAS
and
BRAF
. Nevertheless, there is an unmet need existing to analyze the MTC in the Indian cohort by using in-depth sequencing techniques that go beyond the identification of known therapeutic biomarkers.

MATERIALS AND METHODS

Here, we characterize MTC using integrative whole-exome and whole-transcriptome sequencing of 32 MTC tissue samples. We performed clinically relevant variant analysis, molecular pathway analysis, tumor immune-microenvironment analysis, and structural characterization of
RET
novel mutation.

RESULTS

Mutational landscape analysis shows expected
RET
mutations in 50% of the cases. Furthermore, we observed mutations in known cancer genes like
KRAS
,
HRAS
,
SF3B1
, and
BRAF
to be altered only in the
RET
-negative cohort. Pathway analysis showed differential enrichment of mutations in transcriptional deregulation genes in the
RET
-negative cohort. Furthermore, we observed novel RET kinase domain mutation Y900S showing affinity to RET inhibitors accessed via molecular docking and molecular dynamics simulation.

CONCLUSION
Altogether, this study provides a detailed genomic characterization of patients with MTC of Indian origin, highlighting the possible utility of targeted therapies in this disease.
Assembly of In-Situ Gel Containing Nano-Spanlastics of an Angiotensin II Inhibitor as a Novel Epitome for Hypertension Management: Factorial Design Optimization, In-vitro Gauging, Pharmacokinetics, and Pharmacodynamics Appraisal
Authors: Salem Heba F. , Nafady Mohamed M. , Eissa Essam M. , Abdel-Sattar Hend Hassan , Khallaf Rasha A. 
Published: May 2024

AbstractMore than 1 billion people worldwide suffer from hypertension; therefore, hypertension management has been categorized as a global health priority. Losartan potassium (LP) is an antihypertensive drug with a limited oral bioavailability of about 33% since it undergoes the initial metabolic cycle. Thus, nasal administration is a unique route to overcome first-pass metabolism. The investigation focused on the potential effects of LP-loaded spanlastic vesicles (SNVs) on LP pharmacodynamics and pharmacokinetic parameters, utilizing a thin-film hydration methodology established on a 3122 full factorial design. Entrapment efficiency (EE%) ranged from 39.8 ± 3.87.8 to 83.8 ± 2.92% for LP-SNVs. Vesicle size (VS) varied from 205.5 ± 6.5.10 to 445.1 ± 13.52 nm, and the percentage of LP released after 8 h (Q8h) ranged from 30.8 ± 3.10 to 68.8 ± 1.45%. LP permeated through the nasal mucosa during 24 h and flocculated from 194.1 ± 4.90 to 435.3 ± 13.53 µg/cm2. After twenty-four hours, the optimal LP-SNVs in-situ gel showed 2.35 times more permeation through the nasal mucosa than the LP solution. It also lowered systolic blood pressure, so it is thought to be better than the reference formulation in terms of pharmacodynamics. The pharmacokinetics studies demonstrated that the intranasal LP-SNVs gel boosted its bioavailability approximately 6.36 times compared to the oral LP solution. Our research showed that intranasal LP-SNVs could be a good nanoplatform because they are well-tolerated and have possible pharmacokinetics and pharmacodynamics.
Graphical Abstract
Watching Women: Surveillance and Spectatorship in Early Science Fiction Television
Authors: Dickason Cara 
Published: May 2024

Abstract
This article analyzes episodes of early science fiction anthology series to examine how fears of television's ability to turn its gaze on the home circulated around women, both as objects of surveillance and as television spectators, in the 1950s and 1960s. Media historians have called attention to the importance of women in defining the role of new technologies and have detailed the gendered popular constructions of early television. This article stems from such work, addressing the time when audiences and producers alike were grappling with the imagined potential of television's visuality and liveness to impose visibility on its viewers. Early episodes of Tales of Tomorrow, The Twilight Zone, and The Outer Limits expose anxieties about the power of television as a surveillance mechanism to enact control over audiences. Formally and narratively, these episodes complicate the division between seeing and being seen, particularly for women viewers, while enacting strategies to contain and discipline women. They mobilize women's navigation of visual subjectivity and objectification only to eschew their ability to look and reify their status as objects to be looked at. By taking a closer look at early television surveillance narratives, this article demonstrates how women's television spectatorship has always entailed a complex negotiation of imposed looking relations that paradoxically attempt to deny the female gaze even as they depend on it. Understanding this historical context helps illuminate the gendered power dynamics of spectatorship and visibility in our present media landscape, in which the convergence of TV and surveillance technologies realizes the imagined fears of the mid-twentieth century.
The Feminist Refusal of I May Destroy You
Authors: Ullrich Madeline 
Published: May 2024

Abstract
Michaela Coel's I May Destroy You (HBO, BBC One, 2020) chronicles a first-generation Black woman's struggles in the aftermath of her sexual assault. Set in the United Kingdom, I May Destroy You was well-received by US critics and audiences alike, who rallied around the series as a positive, cohesive, and ultimately feminist example of how a woman productively deals with sexual trauma. These discourses of positive representation used to frame I May Destroy You, however, focus on reinforcing liberal feminist ideals of assimilation, legibility, and respectability in the service of an unexamined “womanhood.” In reality, I May Destroy You uses both plot—as Arabella and her friends attempt to make sense of her rape—and narrative form to deconstruct the identity of “woman,” the term that most television series on “women's issues” and with feminist modes of address have attempted to represent and stabilize since the medium's foundations in the mid-twentieth century. In resisting stable, positive identity categories in a story of sexual violence, Coel's I May Destroy You presents an aberration and an important intervention in a televisual landscape that often relies on access to distinct binaries of male/female, Black/white, and victim/perpetrator to make claims of “accurate” representation—both for sexual assault on television and for women's experience more broadly.
Mammalian pexophagy at a glance
Authors: Bajdzienko Justyna , Bremm Anja 
Published: May 2024

ABSTRACT
Peroxisomes are highly plastic organelles that are involved in several metabolic processes, including fatty acid oxidation, ether lipid synthesis and redox homeostasis. Their abundance and activity are dynamically regulated in response to nutrient availability and cellular stress. Damaged or superfluous peroxisomes are removed mainly by pexophagy, the selective autophagy of peroxisomes induced by ubiquitylation of peroxisomal membrane proteins or ubiquitin-independent processes. Dysregulated pexophagy impairs peroxisome homeostasis and has been linked to the development of various human diseases. Despite many recent insights into mammalian pexophagy, our understanding of this process is still limited compared to our understanding of pexophagy in yeast. In this Cell Science at a Glance article and the accompanying poster, we summarize current knowledge on the control of mammalian pexophagy and highlight which aspects require further attention. We also discuss the role of ubiquitylation in pexophagy and describe the ubiquitin machinery involved in regulating signals for the recruitment of phagophores to peroxisomes.
Demystifying the S3 guidelines: what do they actually mean?
Authors: Raindi Devan S , Chapple Iain , Kebschull Moritz 
Published: May 2024

Recent guidelines have been developed by the European Federation of Periodontology (EFP) and adoloped by the British Society of Periodontology to provide evidence-based guidance for clinicians in the UK to deliver periodontal therapy. To ensure the guidelines are as robust as possible the process of development is carefully structured. This article explores the background behind S3-level guidance for the treatment of periodontitis. CPD/Clinical Relevance: Understanding the process behind guideline formulation allows clinicians to apply them appropriately and confidently within their clinical practice.
An update on the prevention and treatment of peri-implant diseases
Authors: Menhadji Payvand , Lu Emily Ming-Chieh 
Published: May 2024

Peri-implant diseases, especially peri-implantitis, present a growing public health concern owing to their rising prevalence and adverse outcomes, and lead to substantial dental care costs and an overall economic impact. This article outlines the case definitions and diagnoses for peri-mucositis and peri-implantitis and summarizes the EFP S3-level clinical practice guidelines. CPD/Clinical Relevance: Understanding case definitions and diagnoses of peri-implant diseases, including peri-mucositis and peri-implantitis, is essential for dental professionals to provide effective treatment and prevent complications.
EVALUATING SHORT-TERM OUTCOMES POST- INTRA-ARTICULAR CALCANEAL FRACTURE FIXATION VIA A SINUS TARSI APPROACH IN A NON-EXCLUSIVELY SELECTED COHORT
Authors: Davey M. , Stanton P. , Lambert L.A. , McCarton T. , Walsh J. 
Published: May 2024

AimsManagement of intra-articular calcaneal fractures remains a debated topic in orthopaedics, with operative fixation often held in reserve due to concerns regarding perioperative morbidity and potential complications. The purpose of this study was to identify the characteristics of patients who developed surgical complications to inform the future stratification of patients best suited to operative treatment for intra-articular calcaneal fractures and those in whom surgery was highly likely to produce an equivocal functional outcome with potential post-operative complications.MethodsAll patients who underwent open reduction and internal fixation of calcaneal fractures utilizing the Sinus Tarsi approach between March 2014 and July 2018 were identified using theatre records. Patient imaging was used to assess pre- and post-operative fracture geometry with Computed Tomography (CT) used for pre-operative planning. Each patient's clinical presentation was established through retrospective analysis of medical records. Patients provided verbal consent to participation and patient reported outcome measures were recorded using the Maryland Foot Score.ResultsFifty-eight intra-articular calcaneal fractures (fifty-three patients including five bilateral, mean age = 46.91 years) were included. Forty-nine patients were injured as a result of a fall from a height (92.4%). Mean time from presentation to surgery was 3.23 days (range 0–21). Mean Maryland Foot score was found to be 77.6 (+/− 16.22) in forty-five patients. Five patients (9.4%) had wound complications; two superficial (3.7%) and three deep (5.6%).ConclusionIntra-articular fractures of the calcaneus should be considered for surgical intervention in order to improve long-term functional outcomes. The Sinus Tarsi approach provides the potential to decrease the operative complication rate whilst maintaining adequate fixation, however, the decision to surgically manage these fractures should be carefully balanced against the risk of post-operative complications. This increased risk of complication associated with smoking may tip the balance against benefit from surgical management.
Impact of Bone Morphogenetic Protein 7 and Prostaglandin receptors on osteoblast healing and organization of collagen
Authors: Salama Mohammad Ali , Anwar Ismail Asmaa , Islam Md Sofiqul , K. G. Aghila Rani , Al Kawas Sausan , Samsudin A. R. , A. C. Smriti Aryal 
Published: May 2024

Purpose
This study seeks to investigate the impact of co-administering either a Prostaglandin EP2 receptor agonist or an EP1 receptor antagonist alone with a low dose BMP7 on in vitro healing process, collagen content and maturation of human osteoblasts.

Methodology
Human osteoblast cells were used in this study. These cells were cultured and subjected to different concentrations of Prostaglandin EP2 receptor agonist, EP1 receptor antagonist, BMP7, Control (Ct) (Vehicle alone), and various combinations treatments. Cell viability at 24, 48 and 72 hours (h) was evaluated using the XTT assay. A wound healing assay was conducted to observe the migration ability of human osteoblast cells. Additionally, Sirius red staining and Fourier-Transform Infrared Spectroscopy Imaging (FT-IR) was employed to analyze various parameters, including total protein concentration, collagen production, mature collagen concentration, and mineral content.

Results
The combination of low dose BMP7 and Prostaglandin EP2 receptor agonist resulted to the lowest cell viability when compared to both the Ct and individual treatments. In contrast, the Prostaglandin EP1 receptor antagonist alone showed the highest cellular viability at 72 h. In the wound healing assay, the combined treatment of low dose BMP7 with the Prostaglandin EP2 receptor agonist and EP1 receptor antagonist showed a decrease in human osteoblast healing after 24 h. Analysis of FT-IR data indicated a reduction in total protein content, collagen maturity, collagen concentration and mineral content in combination treatment compared to the single or Ct treatments.

Conclusion
The combination of a Prostaglandin EP2 receptor agonist or an EP1 receptor antagonist when combined with low dose BMP7 significantly hinders both human osteoblast healing and collagen maturity/concentration in comparison to low dose BMP7 treatment alone.
Burden and outcome of respiratory morbidities among children and adolescents with sickle cell disease—A retrospective review of emergency presentations in some Nigerian tertiary institutions
Authors: Ibraheem Rasheedat Mobolaji , Abdulkadir Mohammed Baba , Aliu Rasaki , Issa Amudalat , Ibrahim Olayinka Rashid , Bello Abdulafeez Oyesola , Abubakar Fatima Ishaq , Oloyede Iso Precious , Olasinde Yetunde Toyin , Briggs Datonye Christopher , Bashir Muhammad Faruk , Salau Qasim Olakunle , Garba Bilkisu Ilah , Ameen Hafsat Abolore , Suleiman Mohammed Bello , Bewaji Temitayo Olubunmi , Shina Hassan Kamiludeen 
Published: May 2024

Background
Despite the huge burden of sickle cell disease (SCD) among Nigerian children, the burden and outcome of respiratory illnesses remain undocumented. Thus, we aimed to describe the spectrum and outcome of respiratory illnesses among SCD childrenand adolescentadmissions in ten Nigerian tertiary hospitals.

Method
A retrospective review of the SCD admission records of children and adolescents with a confirmed diagnosis of respiratory illnesses from 2012 to 2021 in ten tertiary health facilities across five geopolitical zones in Nigeria was conducted. The data, collectedbetween March and June 2023, included the age, sex, diagnosis, complications, duration and outcome of hospitalization.

Results
Of the 72,333 paediatric admissions, 7,256 (10.0%) had SCD; the proportion of SCD from the total admission ranged from 2.1 to 16.3% in the facilities. Of the 7,256 children and adolescents with SCD, 1,213 (16.7%) had respiratory morbidities. Lower respiratory disease was the most common (70.0%) respiratory entity and the majority were pneumonia (40.1.0%), followed by acute chest syndrome (26.7%). Seventeen (1.4%) patients died; all had lower respiratory diseases [(acute chest syndrome ACS (11, 64.7%), pneumonia; 5, 29.4%, and asthma (1, 5.9%). Based on the proportion of deaths among overall SCD, the 17 death cases contributed 9.4% (95% CI 5.9 to 14.5). Factors associated with deaths included duration of hospitalization less than 72 hours and lower respiratory tract diseases.

Conclusion
Sickle cell disease is a major contributor to hospitalization among Nigerian children and adolescents, with high respiratory morbidity and mortality. Pneumonia and acute chest syndrome were associated with mortality, andthe highest risk of death within the first 72 hours.
Reinvestigating millennial shopping behavior on the sharing economy platform: The moderating role of COVID-19 awareness level
Authors: Ashoer Muhammad , Karim Kasnaeny , Syahnur Muhammad Haerdiansyah , Ating Rashid 
Published: May 2024

Drawing from the Unified Theory of Acceptance and Use of Technology 2 (UTAUT2), this study aims to develop a predictive model concerning the shopping behaviour of millennials within the realm of the sharing economy (SE) mobile application. To accommodate prior research findings while providing novelty, this study integrates hedonic enjoyment and price-saving orientation as predictive factors, alongside the level of COVID-19 awareness as a moderating variable. An online survey was administered, and primary data was collected by distributing an electronic questionnaire link randomly via email and social media platforms. Employing a sampling judgement technique, 260 millennials in Indonesia who utilize the SE (Gojek) mobile app were identified as participants. Results from the PLS-SEM analysis reveal that performance expectancy, effort expectancy, social influence, price-saving orientation, and habits exert a favorable and significant impact on behavioral intentions. Furthermore, habits and behavioral intentions were found to significantly influence the actual usage of the SE app among millennials. Conversely, hedonic enjoyment demonstrated no significant influence on behavioral intentions. Moreover, the moderating role of COVID-19 awareness was observed to both enhance and diminish direct relationships. The implications, both theoretical and practical, along with recommendations for future research, are deliberated upon.
Distress and Coping Strategy among Indonesian Men with Type-2 Diabetes Mellitus
Authors: Nugroho Fajar Ari , Chandra Rico Budhiarta , Laila Nike , Rukia Sera , Kusumastuty Inggita , Cempaka Anggun Rindang , Istifiani Lola Ayu , Latif Atifa Nafia Hasantie 
Published: May 2024

Background: Type 2 diabetes mellitus (T2DM) can have a notable impact on the psychological and physical well-being of individuals, which, in turn, affects the management of the condition. Both men and women experience stress and adopt distinct coping strategies. However, research focusing specifically on T2DM in men is still limited. Purpose: The present study aimed to investigate the distress and coping strategies adopted by outpatient men with T2DM in Malang, East Java, Indonesia.Methods: This study employed a qualitative research design and conducted in-depth interviews with 24 outpatient men with T2DM. The interview guidelines were formulated using the Bahasa Indonesia version of the Diabetes Distress Scale (DDS17) questionnaire, which includes four domains: physician distress, emotional burden, regimen distress, and interpersonal distress. Thematic analysis was performed to analyze the results gathered during the research and compile them into a final report. Results: The study revealed that individuals diagnosed with T2DM experienced a range of emotional and practical difficulties, including feelings of fear, anxiety, and a lack of understanding. Disease burden, a lack of understanding of healthcare services, difficulties managing their diet, routine medication, financial concerns, and fatigue also contributed to the distress. To cope with distress, the informants identified eight distinct coping strategies. Among these, the most effective was receiving support from family members. Additionally, acceptance of the condition, self-management, a positive attitude, understanding of the illness, participation in the diabetes community, spiritual solace, and gaining further knowledge of T2DM were also identified. Conclusion: The findings of this research indicate that men experience eight distinct types of stress and utilize comparable coping strategies associated with T2DM. Emotional distress represents the predominant pressure, while family support constitutes the primary coping strategy. These results are important for nurses and other healthcare professionals in supporting patients.
Therapeutic Doses of Antioxidants Inhibit the Growth and Enhance the Effects Chemotherapy and Radiation Therapy on Cancer Cells by Blocking the Uptake and Metabolism of Glucose and Glutamine
Authors: Prasad Kedar N. 
Published: May 2024

Chemotherapy and radiation therapy have produced increased 5-year survival rate in majority of cancers; however, acute side-effects during treatment and late adverse effects after these therapies remain a major concern of oncologists. To improve further 5-year survival rate and reduce acute and late adverse effects of therapies, a new non-toxic biological approach is needed. Experimental data for such an approach was available for over 25 years. For example, therapeutic doses of antioxidants inhibit the growth of cancer cells and enhances the growth-inhibitory effects of chemotherapeutic agents and x-irradiation on cancer cells but not on normal cells. These exciting observations failed to draw any attention from oncologists, because mechanistic data on therapeutic doses was not available until now. The mechanistic data on preventive doses of antioxidants suggested that they scavenge free radicals. Since one of the mechanisms of action of chemotherapy and radiation therapy on cancer cells is mediated by free radicals, oncologists fear that supplementation with antioxidants may reduce the effectiveness of cancer therapies by neutralizing free radicals. Cancer cells require glucose and glutamine for their survival and proliferation. This review briefly describes the effects of therapeutic doses of antioxidants alone and in combination with chemotherapy and radiation therapy on the growth of neoplastic cells and normal cells. It also presents evidence to show that such doses of antioxidants reduce the uptake and metabolism of glucose and glutamine in cancer cells. We propose that therapeutic doses of multiple antioxidants can selectively kill cancer cells and enhance the growth-inhibitory effects of therapeutic agents on cancer cells without affecting normal cells by inhibiting the uptake and metabolism of glucose and glutamine.