bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue plasminogen activator infusion as a treatment for hemodialysis catheter dysfunction
Authors: Ariyachaipanich Aekarach , Oyejola Olatokunbo , Melhem Arafat , Soundararajan Vinaya , Lerma Edgar , Atassi Wadah , Soundararajan Ramesh 
Published: March 2010

AbstractBACKGROUNDThe formation of a fibrin sheath thrombus is the most common cause of hemodialysis catheter dysfunction (HCD). The infusion of a low‐dose recombinant tissue plasminogen activator (rtPA) has been shown to be safe and effective. However, there is no consensus as to the optimum amount of rtPA.OBJECTIVETo evaluate the safety and efficacy of a low‐dose rtPA infusion for the treatment of fibrin sheath thrombus.METHODSWe did a retrospective review of patients with HCD who were treated with rtPA infusion between August 2006 and August 2007. Each patient had a radiographic evaluation to rule out malposition before administering 1 mg rtPA in 50 mL of normal saline over a 1‐hour period per port (total of 2 mg).RESULTSA total of 74 patients had a total of 86 infusions administered. Their mean age was 62 years, and 52% were male. The clinical success rate, defined as an ability to support at least 1 successful dialysis session, was 95.3%. No major procedure‐related complications occurred. The median catheter patency was 40.5 days.CONCLUSIONRtPA, administered at current study dose, safely and effectively restores catheter function. To our knowledge, this is the lowest dose and shortest infusion time that has been studied.
Breastfeeding patterns of mothers with type 1 diabetes: results from an infant feeding trial
Authors: Sorkio Susa , Cuthbertson David , Bärlund Sonja , Reunanen Antti , Nucci Anita M. , Berseth Carol L. , Koski Katriina , Ormisson Anne , Savilahti Erkki , Uusitalo Ulla , Ludvigsson Johnny , Becker Dorothy J. , Dupré John , Krischer Jeffrey P. , Knip Mikael , Åkerblom Hans K. , Virtanen Suvi M. ,   TRIGR Study Group
Published: March 2010

AbstractBackgroundBoth the initiation and maintenance of breastfeeding have been reported to be negatively affected by maternal type 1 diabetes (T1D). The aim of this study was to prospectively examine the breastfeeding patterns among mothers with and without T1D participating in a large international randomized infant feeding trial (TRIGR).MethodsFamilies with a member affected by T1D and with a newborn infant were invited into the study. Those who had HLA‐conferred genetic susceptibility for T1D tested at birth with gestation > 35 weeks and were healthy were eligible to continue in the trial. Among the 2160 participating children, 1096 were born to women with T1D and 1064 to unaffected women. Information on infant feeding was acquired from the family by frequent prospective dietary interviews.ResultsMost (>90%) of the infants of mothers with and without T1D were initially breastfed. Breastfeeding rates declined more steeply among mothers with than without T1D being 50 and 72% at 6 months, respectively. Mothers with T1D were younger, less educated and delivered earlier and more often by caesarean section than other mothers (p < 0.01). After adjusting for all these factors associated with the termination of breastfeeding, there was no difference in the duration of breastfeeding among mothers with and without T1D.ConclusionsMaternal diabetes status per se was not associated with shorter breastfeeding. The lower duration of breastfeeding in mothers with T1D is largely explained by their more frequent caesarean sections, earlier delivery and lower age and education. Copyright © 2010 John Wiley & Sons, Ltd.
Organometallic Synthesis of β‐CoAl Nanoparticles and β‐CoAl/Al Nanoparticles and Their Behaviour upon Air Exposure (Eur. J. Inorg. Chem. 11/2010)
Authors: Cokoja Mirza , Parala Harish , Birkner Alexander , Fischer Roland A. , Margeat Olivier , Ciuculescu Diana , Amiens Catherine , Chaudret Bruno , Falqui Andrea , Lecante Pierre 
Published: March 2010

AbstractThe cover picture shows the reaction path followed to produce bimetallic CoAl nanoparticles (background image). Organometallic precursors such as AlCp* and [Co(η3‐C8H13)(η4‐C8H12) offer the unique advantage of being easily reduced under dihygrogen, thus enabling access to alloyed β‐CoAl nanoparticles as evidenced by X‐ray absorption and diffusion techniques. As expected, these nanoparticles are highly reactive towards oxygen. The reaction starts with the selective oxidation of Al, with simultaneous segregation of Co. Known in the bulk, this phenomenon is studied for the first time on the nanometer scale. It is clearly evidenced by SQUID measurements: an increase in the ratio of segregated Co atoms is accompanied by an increase in the magnetization of the nanoparticles. To passivate the system, excess aluminum must be decomposed on top of the CoAl nanoparticles. This study evidences the relevance of organometallic chemistry for nanoalloy engineering. Details are discussed in the Short Communication by C. Amiens et al. on p. 1599.
Development of a PDMS‐based microchip electrophoresis device for continuous online in vivo monitoring of microdialysis samples
Authors: Nandi Pradyot , Desai Dhara P. , Lunte Susan M. 
Published: March 2010

AbstractA PDMS‐based microfluidic system for online coupling of microdialysis sampling to microchip electrophoresis with fluorescence detection for in vivo analysis of amino acid neurotransmitters using naphthalene‐2,3‐dicarboxaldehyde and sodium cyanide as the derivatization reagents is described. Fabricating chips from PDMS rather than glass was found to be simpler and more reproducible, especially for chips with complex designs. The microchip incorporated a 20‐cm serpentine channel in which sample plugs were introduced using a “simple” injection scheme; this made fluid handling and injection on‐chip easier for the online system compared with gated or valve‐based injection. The microchip was evaluated offline for the analysis of amino acid standards and rat brain microdialysis samples. Next, precolumn derivatization was incorporated into the chip and in vivo online microdialysis‐microchip electrophoresis studies were performed. The system was employed for the continuous monitoring of amino acid neurotransmitters in the extracellular fluid of the brain of an anesthetized rat. Fluorescein was dosed intravenously and monitored simultaneously online as a marker of in vivo blood–brain barrier permeability. The microdialysis‐microchip electrophoresis system described here will be employed in the future for simultaneous monitoring of changes in blood–brain barrier permeability and levels of amino acid neurotransmitters in the rat stroke model.
Maleopimaric acid anhydride‐bonded silica monolith as chiral stationary phase for separations of phenylthiocarbamyl amino acids by CEC
Authors: Ye Fanggui , Wang Hengshan , Huang Baojun , Zhao Shulin 
Published: March 2010

AbstractA new type of chiral silica monolith was successfully developed for the enantioseparation of phenylthiocarbamyl amino acids (PTC‐D/L‐AAs) by CEC. The bare monolithic silica column was prepared by a sol–gel process and has been modified chemically with aminopropyltrimethoxysilane, followed by immobilization of a chiral selector maleopimaric acid anhydride. Eight pairs of tested amino acids enantiomers including PTC‐D/L‐arginine, PTC‐D/L‐valine, PTC‐D/L‐leucine, PTC‐D/L‐4‐methylphenylalanine, PTC‐D/L‐phenylalanine, PTC‐D/L‐β‐phenylalanine, PTC‐D/L‐tryptophan and PTC‐D/L‐kynurenine were well resolved with high column efficiencies. The influences of organic modifier in mobile phase on apparent retention factor, enantioselectivity factor and resolution were also investigated.
CE assay for simultaneous determination of charged and neutral impurities in dexamphetamine sulfate using a dual CD system
Authors: Wongwan Sudaporn , Sungthong Bunleu , Scriba Gerhard K. E. 
Published: March 2010

AbstractA CE assay for the simultaneous determination of charged and uncharged potential impurities (1S,2S‐(+)‐norpseudoephedrine, 1R,2S‐(−)‐norephedrine, phenylacetone and phenylacetone oxime) of dexamphetamine sulfate including the stereoisomer levoamphetamine was developed and validated. The optimized background electrolyte consisted of a 50 mM sodium phosphate buffer, pH 3.0, containing 80 mg/mL sulfobutylether‐β‐CD and 25 mg/mL sulfated β‐CD. Separations were performed in 40.2/35 cm, 50 μm id fused‐silica capillaries at a temperature of 20°C and an applied voltage of –10 kV. 1R,2S‐(−)‐ephedrine was used as internal standard. The assay was validated in the range of 0.05–1.0% for the related substances and in the range of 0.05–5.0% for levoamphetamine. The LOD was 0.01–0.02% depending on the analyte. The assay also allowed the separation of the E,Z‐stereoisomers of phenylacetone oxime. The effect of the degree of substitution of sulfobutylether‐β‐CD was investigated. In commercial samples of dexamphetamine sulfate between 3.2 and 3.7% of levoamphetamine were found. Furthermore, phenylacetone and phenylacetone oxime could be observed at the LOD, indicating the synthetic origin of the investigated samples.
A coupling strategy based on anisotropic mesh adaptation for solving two‐fluid flows
Authors: Bui Thi Thu Cuc , Frey P. , Maury B. 
Published: March 2010

AbstractWe propose a coupling strategy for solving efficiently bifluid flows based on the Stokes equations. Our approach relies on a level set formulation of the interface‐capturing problem, and involves a finite element discretization for the fluid resolution, the method of characteristics for solving the advection of the interface and the anisotropic mesh adaptation of the computational domain in the vicinity of the interface for better accuracy. Copyright © 2010 John Wiley & Sons, Ltd.
Three‐dimensional automatic local remeshing for two or more hybrid meshes
Authors: Ito Yasushi , Shih Alan M. , Soni Bharat K. 
Published: March 2010

AbstractIn this paper, an automatic local remeshing method is proposed for merging two or more hybrid meshes consisting of prisms, hexahedra, pyramids and tetrahedra to reduce users' efforts and turnaround time for mesh generation as an extension of the local remeshing method for small devices by Ito et al. (AIAA J. 2009; 47(5):1270–1276. DOI: 10.2514/1.40875). Two types of relationships between two volume meshes are considered: (1) a volume mesh containing the other smaller volume mesh inside (parent–child relationship) and (2) a volume mesh connecting to the other volume mesh (sibling relationship). The primary advantage of the proposed method over the existing ones is that it can handle the geometries in the two hybrid meshes intersecting each other. Two examples are shown to demonstrate the local remeshing method. Copyright © 2010 John Wiley & Sons, Ltd.
Voice‐related quality of life (V‐RQOL) outcomes in laryngectomees
Authors: Moukarbel Roger V. , Doyle Philip C. , Yoo John H. , Franklin Jason H. , Day Adam M. B. , Fung Kevin 
Published: March 2010

AbstractBackground.Laryngeal cancer has a significant impact on patients. This study compared the Voice‐Related Quality of Life (V‐RQOL) outcomes specific to 3 different postlaryngectomy voice rehabilitation methods.Methods.We conducted a retrospective review of 75 patients with laryngectomy from our V‐RQOL questionnaire database.Results.The database included 18 electrolaryngeal speech (ELS), 15 esophageal speech (ES), and 42 tracheoesophageal speech (TES) patients. Pairwise comparisons of V‐RQOL outcomes showed that TES was perceived to be better than ELS (p < .001). ES was perceived as better than ELS, but this was driven by a difference in the total and social–emotional V‐RQOL scores (p < .05). There was no difference between TES and ES groups. Only ELS showed a positive correlation with time after surgery and older age.Conclusions.Patients using TES had similar V‐RQOL outcomes compared to ES and both performed significantly better than ELS. For ELS, the total V‐RQOL score was better with longer time after surgery and older age. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Expression and function of iron‐regulatory proteins in retina
Authors: Gnana‐Prakasam Jaya P. , Martin Pamela M. , Smith Sylvia B. , Ganapathy Vadivel 
Published: March 2010

AbstractIron is essential for cell survival and function; yet excess iron is toxic to cells. Therefore, the cellular and whole‐body levels of iron are regulated exquisitely. At least a dozen proteins participate in the regulation of iron homeostasis. Hemochromatosis, a genetic disorder of iron overload, is caused by mutations in at least five genes, namely HFE, hemojuvelin, Transferrin receptor 2, ferroportin, and hepcidin. Retina is separated from systemic circulation by inner and outer blood‐retinal barriers; therefore it is widely believed that this tissue is immune to changes in systemic circulation. Even though hemochromatosis is associated with iron overload and dysfunction of a variety of systemic organs, little is known on the effects of this disease on the retina. Recent studies have shown that all five genes that are associated with hemochromatosis are expressed in the retina in a cell type‐specific manner. The retinal pigment epithelium, which forms the outer blood‐retinal barrier, expresses all of these five genes. It is therefore clearly evident that iron homeostasis in the retina is maintained locally by active participation of various iron‐regulatory proteins. Excess iron is detrimental to the retina as evidenced from human studies and from mouse models of iron overload. Retinal iron homeostasis is disrupted in various clinical conditions such as hemochromatosis, aceruloplasminemia, age‐related macular degeneration, and bacterial and viral infections. © 2010 IUBMB IUBMB Life, 62(5): 363–370, 2010
Modification of polymer networks with bone sialoprotein promotes cell attachment and spreading
Authors: Chan Wailen D. , Goldberg Harvey A. , Hunter Graeme K. , Dixon S. J. , Rizkalla Amin S. 
Published: March 2010

AbstractBiomaterials used for tissue engineering scaffolds act as temporary substrates, on which cells deposit newly synthesized extracellular matrix. In cartilage tissue engineering, polycaprolactone/poly(2‐hydroxyethyl methacrylate) (PCL/pHEMA) polymer blends have been used as scaffold materials, but their use in osseous tissue engineering has been more limited. The objective of this study was to evaluate modification of PCL/pHEMA surfaces with bone sialoprotein (BSP), an extracellular matrix protein important in regulating osseous tissue formation. Modification of surfaces with BSP significantly enhanced osteoblastic cell attachment and spreading, without compromising proliferation. Thus, BSP‐immobilization may be a useful strategy for optimizing scaffolds for skeletal tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Carboxymethyl‐chitin promotes chondrogenesis by inducing the production of growth factors from immune cells
Authors: Kariya Hiroyuki , Yoshihara Yusuke , Nakao Yumiko , Sakurai Nobuko , Ueno Masaru , Hashimoto Masahito , Suda Yasuo 
Published: March 2010

AbstractMany techniques have been tested for their ability to restore cartilage defects, but several problems still remain in the complete healing of injured cartilage. In our previous study, we found that a carboxymethyl‐chitin/β‐tricalcium phosphate (CM‐chitin/β‐TCP) composite induced cartilage regeneration in the osteochondral defects of rabbits in vivo. We also found that CM‐chitin stimulated peritoneal exudate cells (PEC) in mice and induced several kinds of inflammatory cytokines and transforming growth factor beta‐1 (TGF‐β1). In this study, we examined whether CM‐chitin is responsible for the induction of chondrogenesis via the production of TGF‐β1 in vitro. The murine pluripotent cell line C3H10T1/2 was maintained as a micromass culture in conditioned medium prepared from PEC stimulated with and without CM‐chitin. CM‐chitin–conditioned medium induced RNA expression of the chondrogenic‐factor Sox9 and the matrix proteins aggrecan, Col2a1, and Comp. Their expression levels were decreased in the presence of anti‐TGF‐β1 antibody. The micromass tissues cultured in CM‐chitin conditioned medium at day 21 were clearly stained by Toluidine blue or Alcian blue (histological staining) and collagen II antibody (immunohistological staining), showing the expression of acidic glycosaminoglycan and type II collagen. Similar results were observed in micromass tissue stimulated with TGF‐β1 as a positive control. However, no chondrogenesis occurred when CM‐chitin was added directly to a C3H10T1/2 cell culture. These results indicated that CM‐chitin is a potent inducer of chondrogenesis via the induction of TGF‐β1 in immune cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Concentrated collagen‐chondroitin sulfate scaffolds for tissue engineering applications
Authors: Liang Wan‐Hsiang , Kienitz Brian L. , Penick Kitsie J. , Welter Jean F. , Zawodzinski Thomas A. , Baskaran Harihara 
Published: March 2010

AbstractCollagen‐chondroitin sulfate biomaterial scaffolds have been used in a number of tissue‐engineered products under development or in the clinics. In this article, we describe a new approach based on centrifugation for obtaining highly concentrated yet porous collagen scaffolds. Water uptake, chondroitin sulfate retention, morphology, mechanical properties, and tissue‐engineering potential of the concentrated scaffolds were investigated. Our results show that the new approach can lead to scaffolds containing four times as much collagen as that in conventional unconcentrated scaffolds. Further, water uptake in the concentrated scaffolds was significantly greater while chondroitin sulfate retention in the concentrated scaffolds was unaffected. The value of mean pore diameter in the concentrated scaffolds was smaller than that in the unconcentrated scaffolds and the walls of the pores in the former comprised of a continuous sheet of collagen. The mechanical properties measured as moduli of elasticity in compression and tension were improved by as much as 30 times in the concentrated scaffolds. In addition, our tissue culture results with human mesenchymal stem cells and foreskin keratinocytes show that the new scaffolds can be used for cartilage and skin tissue–engineering applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Characterization of the chemotactic and mitogenic response of SMCs to PDGF‐BB and FGF‐2 in fibrin hydrogels
Authors: Ucuzian Areck A. , Brewster Luke P. , East Andrea T. , Pang Yongang , Gassman Andrew A. , Greisler Howard P. 
Published: March 2010

AbstractThe delivery of growth factors to cellularize biocompatible scaffolds like fibrin is a commonly used strategy in tissue engineering. We characterized smooth muscle cells (SMC) proliferation and chemotaxis in response to PDGF‐BB and FGF‐2, alone and in combination, in 2D culture and in 3D fibrin hydrogels. While both growth factors induced an equipotent mitogenic response in 2D culture, only FGF‐2 was significantly mitogenic for SMCs in 3D culture. Only PDGF‐BB was significantly chemotactic in a modified Boyden chamber assay. In a 3D assay of matrix invasion, both growth factors induced an invasive response into the fibrin hydrogel in both proliferating and nonproliferating, mitomycin C (MMC) treated cells. The invasive response was less attenuated by the inhibition of proliferation in PDGF‐BB stimulated cells compared with FGF‐2 stimulated cells. We conclude that SMCs cultured in fibrin hydrogels have a more robust chemotactic response to PDGF‐BB compared with FGF‐2, and that the response to FGF‐2 is more dependent on cell proliferation. Delivery of both growth factors together potentiates the chemotactic, but not mitogenic response to either growth factor alone. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Endothelialization of PVA/gelatin cryogels for vascular tissue engineering: Effect of disturbed shear stress conditions
Authors: Vrana Nihal Engin , Cahill Paul A. , McGuinness Garrett B. 
Published: March 2010

AbstractMechanically, poly(vinyl alcohol) (PVA)‐based cryogels are extremely well suited for vascular tissue engineering applications. However, their surface properties lead to a slow rate of endothelialization, and the mode of cell attachment leaves the endothelium susceptible to removal under physiological shear stress conditions. In this study, abrupt and ramped disturbed shear stress conditions created by a turbulent orbital flow were used to examine endothelialization on PVA/gelatin cryogels. Cell proliferation rate and apoptosis were evaluated by fluorescent activated cell sorter (FACS) analysis, and the expression of cell‐adhesion molecules was used to evaluate the response of cells on cryogels to static and shear conditions by real‐time polymerase chain reaction (RT‐PCR). Application of a ramped shear stress had a profound effect on endothelial cell proliferation (22.30 ± 0.20‐fold increase), necrosis (eliminated), apoptosis (1.04 ± 0.18 increase), and overall facilitation of endothelialization while concomitantly increasing nitric oxide (NO) synthesis levels. Ramped shear stress was also effective in helping the retention of the endothelial cells on the cryogel surface, whereas abrupt application caused widespread removal. Under static conditions, Selectin‐P expression decreased, whereas both inter‐cellular adhesion molecule (ICAM) and platelet endothelial cell adhesion molecule (PECAM)‐I expression increased on cryogels over a 10‐day culture period. Under both shear stress conditions, Selectin‐P expression was decreased both on cryogels and tissue culture polystyrene (TCPS). Controlled application of disturbed shear stress shortens endothelialization times on cryogel surfaces, in contrast to the established antiproliferative effect of shear stress caused by laminar flow, without compromising their functionality. This demonstrates how such mechanical stimuli can be exploited to alter cellular behavior and facilitate the required outcomes for tissue engineering applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Sonography of the normal greater occipital nerve and obliquus capitis inferior muscle
Authors: Suk Cho John Chin , Haun Daniel W. , Kettner Norman W. , Scali Frank , Clark Thomas B. 
Published: March 2010

AbstractBackground.To use sonography to measure the cross‐sectional area (CSA) of the greater occipital nerve (GON) and the adjacent obliquus capitis inferior muscle (OCI) in normal subjects.Methods.Data from 30 asymptomatic subjects between the ages of 22 and 35 were collected. CSA and circumference of the GON and CSA of OCI were measured using sonography. Interexaminer reliability analysis was performed using the intraclass correlation coefficient.Results.The CSA of the GON and OCI were 2 mm2 ± 1 mm2 and 1.86 cm2 ± 0.51 cm2, respectively. The average circumference of the GON was 4.8 mm ± 1.3 mm. The interexaminer reliability of the measurements was excellent with intraclass correlation coefficient coefficients of 0.91, 0.84, and 0.73 for the GON CSA, GON circumference, and OCI CSA, respectively.Conclusion.We report the normal values of the CSA of the GON and OCI. Knowledge of these normal values may facilitate the diagnosis of GON entrapment and provide outcome measures in therapeutic interventions. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Synthesis of 14‐aryl‐14H‐dibenzo[a,j]xanthenes catalyzed by acyclic acidic ionic liquids
Authors: Fang Dong , Liu Zu‐Liang 
Published: March 2010

Abstract
magnified image Some recyclable acyclic SO3H‐functionalized ionic liquids have been used as novel catalysts for the synthesis of 14‐aryl‐14H‐dibenzo[a,j]xanthenes via the one‐pot condensation of β‐naphthol and aromatic aldehydes in aqueous medium. The condensation reaction was accomplished successfully with various aromatic aldehydes with good to excellent yields ranged from 86 to 96% within 5–30 min. After the reaction, the products could simply be separated from the catalysts by filtration. When separated from the reaction mixture, the catalysts could be recycled and reused for several times without noticeably reducing catalytic activity. The methodology gives the advantages of high yields, short reaction time, and easy work‐up procedure. J. Heterocyclic Chem., (2010).