bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lyophilized cell-free supernatants of Limosilactobacillus fermentum T0701 exhibited antibacterial activity against Helicobacter pylori
Authors: Sornsenee Phoomjai , Surachat Komwit , Wong Thanawin , Kaewdech Apichat , Saki Morteza , Romyasamit Chonticha 
Published: June 2024

AbstractHelicobacter pylori is a prominent gastrointestinal pathogen associated with various gastrointestinal illnesses. It presents substantial health risks due to its antibiotic resistance. Therefore, it is crucial to identify alternative treatments for H. pylori infections. Limosilactobacillus spp exhibit probiotic properties with beneficial effects in humans; however, the mechanisms by which it counteracts H. pylori infection are unknown. This study aimed to evaluate the potential of Limosilactobacillus fermentum T0701 lyophilized cell-free supernatants (LCFS) against H. pylori. The LCFS has varying antimicrobial activities, with inhibition zones of up to 10.67 mm. The minimum inhibitory concentration and minimum bacterial concentration of LCFS are 6.25–25.00 mg/mL and 6.25 mg/mL to > 50.00 mg/mL, respectively, indicating its capability to inhibit H. pylori. There is morphological damage observed in H. pylori treated with LCFS. Additionally, H. pylori adhesion to AGS cells (human gastric adenocarcinoma epithelial cells) reduces by 74.23%, highlighting the LCFS role in preventing bacterial colonization. Moreover, LCFS exhibits no cytotoxicity or morphological changes in AGS cells, and with no detected virulence or antimicrobial resistance genes, further supporting its safety profile. L. fermentum T0701 LCFS shows promise as a safe and effective non-toxic agent against H. pylori, with the potential to prevent gastric colonization.
Development and validation of an interpretable model for predicting sepsis mortality across care settings
Authors: Lee Young Seok , Han Seungbong , Lee Ye Eun , Cho Jaehwa , Choi Young Kyun , Yoon Sun-Young , Oh Dong Kyu , Lee Su Yeon , Park Mi Hyeon , Lim Chae-Man , Moon Jae Young ,   the Korean Sepsis Alliance (KSA) Investigators, Hong Sang‑Bum , Hong Suk‑Kyung , Suh Gee Young , Jeon Kyeongman , Ko Ryoung‑Eun , Cho Young‑Jae , Lee Yeon Joo , Lim Sung Yoon , Park Sunghoon , Heo Jeongwon , Lee Jae‑myeong , Kim Kyung Chan , Chang Youjin , Lee Sang‑Min , Cho Woo Hyun , Kwak Sang Hyun , Lee Heung Bum , Ahn Jong‑Joon , Seong Gil Myeong , Lee Song I. , Park Tai Sun , Lee Su Hwan , Choi Eun Young , Kang Hyung Koo 
Published: June 2024

AbstractThere are numerous prognostic predictive models for evaluating mortality risk, but current scoring models might not fully cater to sepsis patients’ needs. This study developed and validated a new model for sepsis patients that is suitable for any care setting and accurately forecasts 28-day mortality. The derivation dataset, gathered from 20 hospitals between September 2019 and December 2021, contrasted with the validation dataset, collected from 15 hospitals from January 2022 to December 2022. In this study, 7436 patients were classified as members of the derivation dataset, and 2284 patients were classified as members of the validation dataset. The point system model emerged as the optimal model among the tested predictive models for foreseeing sepsis mortality. For community-acquired sepsis, the model’s performance was satisfactory (derivation dataset AUC: 0.779, 95% CI 0.765–0.792; validation dataset AUC: 0.787, 95% CI 0.765–0.810). Similarly, for hospital-acquired sepsis, it performed well (derivation dataset AUC: 0.768, 95% CI 0.748–0.788; validation dataset AUC: 0.729, 95% CI 0.687–0.770). The calculator, accessible at https://avonlea76.shinyapps.io/shiny_app_up/, is user-friendly and compatible. The new predictive model of sepsis mortality is user-friendly and satisfactorily forecasts 28-day mortality. Its versatility lies in its applicability to all patients, encompassing both community-acquired and hospital-acquired sepsis.
Partitioning into ER membrane microdomains impacts autophagic protein turnover during cellular aging
Authors: Prokisch Simon , Büttner Sabrina 
Published: June 2024

AbstractEukaryotic membranes are compartmentalized into distinct micro- and nanodomains that rearrange dynamically in response to external and internal cues. This lateral heterogeneity of the lipid bilayer and associated clustering of distinct membrane proteins contribute to the spatial organization of numerous cellular processes. Here, we show that membrane microdomains within the endoplasmic reticulum (ER) of yeast cells are reorganized during metabolic reprogramming and aging. Using biosensors with varying transmembrane domain length to map lipid bilayer thickness, we demonstrate that in young cells, microdomains of increased thickness mainly exist within the nuclear ER, while progressing cellular age drives the formation of numerous microdomains specifically in the cortical ER. Partitioning of biosensors with long transmembrane domains into these microdomains increased protein stability and prevented autophagic removal. In contrast, reporters with short transmembrane domains progressively accumulated at the membrane contact site between the nuclear ER and the vacuole, the so-called nucleus-vacuole junction (NVJ), and were subjected to turnover via selective microautophagy occurring specifically at these sites. Reporters with long transmembrane domains were excluded from the NVJ. Our data reveal age-dependent rearrangement of the lateral organization of the ER and establish transmembrane domain length as a determinant of membrane contact site localization and autophagic degradation.
An economic framework for assessing the impact of domestic mining policies on affordable energy transition
Authors: Fikru Mahelet G. 
Published: June 2024

AbstractThe global transition towards sustainable energy sources necessitates a delicate balance between incentivizing domestic mineral inputs and ensuring affordable energy transition. This paper investigates a diverse set of policies aimed at promoting domestic mining and their implications for achieving an affordable energy transition. Through a comprehensive economic framework, we analyze the effects of demand and supply-side policies on import reliance, production costs, and the overall progress of the energy transition. By examining various scenarios and their outcomes, we provide insights into the potential challenges and opportunities associated with designing mineral policies that facilitate both domestic mining growth and affordable clean energy technologies. Our findings highlight the importance of striking a balance between promoting domestic resources and ensuring affordability in the pursuit of a sustainable energy future.
Artificially designed synthetic promoter for a high level of salt induction using a cis-engineering approach
Authors: Bhadouriya Sneha Lata , Karamchandani Arti Narendra , Nayak Namitha , Mehrotra Sandhya , Mehrotra Rajesh 
Published: June 2024

AbstractThis work aimed to design a synthetic salt-inducible promoter using a cis-engineering approach. The designed promoter (PS) comprises a minimal promoter sequence for basal-level expression and upstream cis-regulatory elements (CREs) from promoters of salinity-stress-induced genes. The copy number, spacer lengths, and locations of CREs were manually determined based on their occurrence within native promoters. The initial activity profile of the synthesized PS promoter in transiently transformed N. tabacum leaves shows a seven-fold, five-fold, and four-fold increase in reporter GUS activity under salt, drought, and abscisic acid stress, respectively, at the 24-h interval, compared to the constitutive CaMV35S promoter. Analysis of gus expression in stable Arabidopsis transformants showed that the PS promoter induces over a two-fold increase in expression under drought or abscisic acid stress and a five-fold increase under salt stress at 24- and 48-h intervals, compared to the CaMV35S promoter. The promoter PS exhibits higher and more sustained activity under salt, drought, and abscisic acid stress compared to the constitutive CaMV35S.
Dendrite-free solid-state lithium batteries enabled by a fluorine doped Li7La3Zr2O12 composite electrolyte and LiAlF4 interphase
Authors: Mao Yuezhen , Mi Fanghui , Wang Tianyuan , Zhang Zhijun , Sun Chunwen 
Published: June 2024

LLZOF0.2 CSE and Li@AlF3 anode are constructed to enhance the performance of SSLMBs. Theoretical calculations proved that LLZOF0.2 promotes the dissociation of LiTFSI. The full cells exhibit exceptional electrochemical performance and stability.
The Effects of Experimental Sleep Extension in Middle-to-Older-Aged Healthy Sleepers
Authors: Reynolds Alexandria M. , Seymour Zachary R. , Iftikhar Imran H. , Burnette M. Michele , Vendemia Jennifer M. C. , Youngstedt Shawn D. 
Published: June 2024

Abstract
Objectives To determine the feasibility for middle-aged and older adults to extend their time in bed by 2 h per night for 3 consecutive weeks. Other aims were to examine the effects of sleep extension on mood, cognitive performance, and cardiovascular health.
Methods Ten healthy middle-aged to older adults (9 women; M = 65.20 ± 4.78 years) who reported regularly sleeping 6 to 8 h per night participated in a randomized controlled cross-over study: 3 weeks of both habitual sleep and extended sleep (1-week recovery between treatments). Participants were asked to spend 2 additional hours in bed per night during sleep extension. Cognitive (e.g., errors, response time), psychological (e.g., depression, anxiety, mood), and physiological measures (e.g., inflammation, glucose, triglycerides, blood pressure) were assessed.
Results Compared with habitual sleep, time in bed increased 81.63 ± 33.11 min and total sleep time increased 66.33 ± 28.64 min during sleep extension; these variables did not significantly change during baseline or the habitual sleep treatment. No significant treatment differences were found in the cognitive, psychological, or physiological measures.
Discussion Neither significant positive nor negative effects of sleep extension were found for any of the variables. In terms of feasibility, it was difficult for the participants to extend their time in bed and, subsequently, attain more sleep by the targeted amount. Sleep extension by a greater degree or longer period of times might be more likely to elicit positive or negative effects.
Effect of Fluid Therapy on Acid–Base Balance in Patients Undergoing Clipping for Ruptured Intracranial Aneurysm: A Prospective Randomized Controlled Trial
Authors: Sundaram Senthil Kumaran , Panda Nidhi Bidyut , Kaloria Narender , Soni Shiv Lal , Mahajan Shalvi , Karthigeyan Madhivanan , Pattanaik Smita , Singh Sheetal , Dey Sumit , Pal Arnab , Tripathi Manjul 
Published: June 2024

Abstract
Objectives Neurosurgical patients often receive 0.9% normal saline (NS) during the perioperative period. Theoretically, a balanced salt solution (BSS) is better than 0.9% saline. We compared the effects of two different fluids on acid–base balance, renal function, and neurological outcome in patients who underwent clipping following subarachnoid hemorrhage from a ruptured intracranial aneurysm.
Materials and Methods Patients in group NS (n = 30) received 0.9% saline and group BSS (N = 30) received BSS (Plasmalyte-A) in the perioperative period for 48 hours. Comparison of arterial pH, bicarbonate, and base deficit measured preoperatively, intraoperatively (first and second hour), and postoperatively (at 24 and 48 hours) was the primary outcome of the study. The secondary outcome compared serum electrolytes, renal function tests, urine neutrophil gelatinase-associated lipocalin (NGAL), serum cystatin C, and the neurological outcome using modified Rankin score (MRS) at discharge, 1, and 3 months.
Results In group NS, significantly low pH at 1-hour intraoperative period was seen compared with group BSS (7.37 ± 0.06 vs. 7.40 ± 0.05, p = 0.024). The bicarbonate level in group NS was significantly lower and the base deficit was higher at second intraoperative hour (bicarbonate: 17.49 vs. 21.99 mEq/L, p = 0.001; base deficit: 6.41 mmol/L vs. 1.89 mmol/L, p = 0.003) and at 24 hours post-surgery (bicarbonate: 20.38 vs. 21.96 mEq/L, p = 0.012; base deficit: 3.56 mmol/L vs. 2.12 mmol/L, p = 0.034)). Serum creatinine was higher in group NS at 24 hours (0.66 vs. 0.52 mg/dL, p = 0.013) and 48 hours (0.62 vs. 0.53 mg/dL, p = 0.047). Serum urea, electrolytes, cystatin, urine NGAL, and MRS were comparable.
Conclusion In neurosurgical patients undergoing clipping for ruptured intracranial aneurysm, using a BSS during the perioperative period is associated with a better acid–base and renal profile. However, the biomarkers of kidney injury and long-term outcomes were comparable.
Primary CNS Melanoma of Meckel's Cave: A Rare Case with Literature Review
Authors: Makkina Charan , Joseph Jeena , Raju Krishna Prabhu , Jayachandran Ranjani , Chacko Geeta 
Published: June 2024

AbstractMeckel's cave is a dural recess in the posteromedial portion of the middle cranial fossa, serving as a conduit for the trigeminal nerve and hosting various pathologies. The radiological diagnosis of Meckel's cave pathologies is often challenging, especially when they are atypical and rarely encountered. Here, we discuss the case of a 41-year-old woman who presented with right hemifacial pain, numbness, and binocular diplopia. Imaging features suggested a T2 hypointense, T1 hyperintense, and nonenhancing mass in the right Meckel's cave. Intraoperatively, an extra-axial black mass was observed, suggestive of melanoma, which was radically excised. Further postoperative workup and biopsy revealed it was a primary central nervous system (CNS) malignant melanoma, an exceedingly rare condition. Due to the rarity of the disease, a consensus regarding treatment regimens is lacking. This case report underscores the significance of considering uncommon diagnoses when faced with unusual radiological findings and emphasizes the importance of aggressive surgical resection and the evolving landscape of adjuvant treatments for primary CNS melanomas.
Computational study of laser-produced plasma, EUV generation, and the impact of magnetic fields
Authors: Kim J. , Bally-Grandvaux M. , Beg F. N. 
Published: June 2024

Efficient generation of 13.5 nm light with increased conversion efficiency and output power is important for Extreme Ultraviolet (EUV) lithography applications. In this study, we present a computational investigation of plasma dynamics and EUV generation from laser-driven plasma, with specific focus on the influence of magnetic fields, ranging up to 50 T. Simulations show that the plasma expansion is restricted based on the direction and strength of the magnetic field, resulting in an anisotropic plasma confinement, which in turn allows for radiation escape with a reduced loss. Moreover, angle-dependent measurements show an increase in in-band EUV (2% bandwidth around 13.5 nm) yield, reaching a peak enhancement of up to 40% when a magnetic field is applied, particularly when it is oriented perpendicular to the laser axis. The ability to control plasma dynamics by magnetic field offers exciting prospects for optimizing EUV radiation sources.
ASR-based system for promoting pronunciation
Authors: Sariani Sariani , Khairat Mutia El , Haslina Welsi , Baetty Baety 
Published: June 2024

AbstractIn developing English as a Lingua Franca, educators and researchers must employ new methods in language acquisition to make the learners internationally intelligible and comprehensible. This study aimed to determine the implication of infinite access to Automatic Speech Recognition (ASR)-based language-learning program promoting pronunciation skills acquisition of vocational higher education students. In this study, the students learned English as Lingua Franca (ELF) through a collaborative approach. It applied a qualitative approach with 67 first-year university students from three intact classes as participants. One class of 24 students was assigned as the first group utilising the ASRBoldVoiceas the additional learning materials in their speaking class. In contrast, the other two classes of 43 students were set as the second and third groups carrying out conventional learning procedures. The development of participants’ utterances was analysed in terms of fluency, completeness, and accuracy at pre- and post-test. In order to support the obtained data, a semi-structured interview was performed right after the post-test. The result showed that the pronunciation skill of the experimental group was significantly improved in particular phones of pronunciation: /ch/, /th/, /sh/, and /j/. The analysis of the interview data confirmed that the students gained substantial improvement with regard to their pronunciation and communicative competence compared to the conventional learning process.
Challenges and potential of quasi-experimental studies in cognitive linguistics applied to language teaching and
learning
Authors: Martín-Gascón Beatriz 
Published: June 2024

Abstract
This review study focuses on the challenges faced when conducting effects-of-instruction research in Applied
Cognitive Linguistics. It offers an overview of relevant research that has sought to demonstrate the effectiveness of a
cognitive-based approach to second language (L2) pedagogy and provides illustrations from previous work in L2 Spanish by the
author and colleagues of some of the factors that can influence findings in quasi-experimental research. More specifically, the
study addresses some of the difficulties encountered in the design of materials and assessment tests, during the pedagogical
intervention and the data collection and data analysis phases. These include choice of assessment, test effects, sample size,
withdrawal, and time-on-task, among others. Along with these methodological issues, a discussion of possible solutions as well as
pedagogical and methodological implications are discussed.
Theoretical insights into thermal transport and structural stability mechanisms of triaxial compressed methane hydrate
Authors: Chen 陈 Dong-Sheng 东升 , Miao 缪 Ting-Ting 婷婷 , Chang 常 Cheng 程 , Guo 郭 Xu-Yang 旭洋 , Guan 关 Meng-Yan 梦言 , Ji 姬 Zhong-Li 忠礼 
Published: June 2024

Abstract
The heat transfer and stability of methane hydrate in reservoirs have a direct impact on the drilling and production efficiency of hydrate resources, especially in complex stress environments caused by formation subsidence. In this study, we investigated the thermal transport and structural stability of methane hydrate under triaxial compression using molecular dynamics simulations. The results suggest that the thermal conductivity of methane hydrate increases with increasing compression strain. Two phonon transport mechanisms were identified as factors enhancing thermal conductivity. At low compressive strains, a low-frequency phonon transport channel was established due to the overlap of phonon vibration peaks between methane and water molecules. At high compressive strains, the filling of larger phonon bandgaps facilitated the opening of more phonon transport channels. Additionally, we found that a strain of −0.04 is a watershed point, where methane hydrate transitions from stable to unstable. Furthermore, a strain of −0.06 marks the threshold at which the diffusion capacities of methane and water molecules are at their peaks. At a higher strain of −0.08, the increased volume compression reduces the available space, limiting the diffusion ability of water and methane molecules within the hydrate. The synergistic effect of the strong diffusion ability and high probability of collision between atoms increases the thermal conductivity of hydrates during the unstable period compared to the stable period. Our findings offer valuable theoretical insights into the thermal conductivity and stability of methane hydrates in reservoir stress environments.
Description of moment of inertia and the interplay between anti-pairing and pairing correlations in 244Pu and 248Cm*
Authors: Dadwal Anshul , He 贺 Xiao-Tao 晓涛 
Published: June 2024

Abstract
A variable moment of inertia (VMI) inspired interacting boson model (IBM), which includes many-body interactions and a perturbation possessing

(5) (or

(5)) symmetry, is used to investigate the rotational bands of the

mass region. A novel modification is introduced, extending the Arima coefficient to the third order. This study is dedicated to the quantitative analysis of evolving trends in intraband γ-transition energy as well as the kinematic and dynamic moments of inertia (MoIs) within the rotational bands of

Pu and

Cm. The computed outcomes exhibit an exceptional degree of agreement with experimental observations across various conditions. The significance of including a higher-order Arima coefficient is further examined by contrasting it with the previously proposed model. The calculated results demonstrate the significance of both the anti-pairing and pairing effects in the evolution of the dynamic MoI. Additionally, these insights reveal the importance of a newly introduced parameter in accurately depicting complex nuclear behaviors, such as back-bending, up-bending, and downturn in the MoI.
Metallurgical and mechanical properties of marine grade AA5356 using wire arc additive manufacturing
Authors: A Muthukumaran , Jeyakumar S , Jayakumar K 
Published: June 2024

Abstract
In the current work, a Gas metal arc welding (GMAW)-based Wire Arc Additive Manufacturing (WAAM) procedure was used to build a wall construction of measuring Aluminium alloy (AA) AA5356 on an AA5083 base plate. The microstructure and mechanical properties of AA5356 were examined at two places along the wall structure’s horizontal deposition direction and in two deposition orientations (horizontal and vertical). Optical microscopy, SEM, EDAX, and fractographical examinations were used to analyse the microstructure. Tensile and microhardness tests were performed at two wall locations to evaluate mechanical parameters. A microstructure analysis reveals a mixture of columnar grain structure and coarser intermetallics in the remelting zone, with finer granular structure in the central region. The horizontal direction of AA5356 deposition exhibited a highest elongation and tensile strength of 4.4% and 249 MPa than the vertical direction. For the horizontal and vertical orientations, the average microhardness values were determined to be 80 HV and 72 HV, respectively. Fracture analysis of the tensile samples showed that the deposited metal had a ductile mode of failure with a predominance of dimples with tearing shape. This study provides valuable insights into constructing wall structures and analyzing their mechanical properties.