bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acidic Apple Snails: Effects of Climate Change on the Mechanical Properties of an Invasive Gastropod
Authors: Cretini Cody P , Galloway Katherine A 
Published: May 2024

Synopsis
Climate change can directly and indirectly affect species distribution. Warming may allow for invasive species, such as apple snails, to migrate to higher latitudes where temperatures are more conducive to their survival and invasion success. Higher temperatures and lower pH ranges have been previously documented to affect the form and function of calcium carbonate shells, which serve many functions, including protection from predators and thermoregulation. This study aimed to quantify differences in the morphology and mechanical properties of invasive apple snail, Pomacea maculata, shells after altering temperature and pH. We mechanically tested shells among three five-week treatments: control, higher temperature, and lower pH. Ultimate Strength increased in shells that were exposed to higher temperatures, but Young’s Modulus and Peak Load did not differ among control, temperature, and pH treatments. Apple snails in higher temperature tanks increased their shell length over the five-week trials. Although snail morphometrics did not differ between sexes, male shells exhibited a higher Peak Load, Young’s Modulus, and Ultimate Strength compared to female shells. Our findings are consistent with previous gastropod studies, in that a lower pH is associated with a decrease in shell size, and higher temperatures yield larger snail shells with a higher ultimate strength. Peak Load did not significantly differ among treatments, which suggests that the cross-sectional area is relatively important when considering this species mechanical performance today and in future climates. Due to the intense nutritional and calcium demands of egg production, female snails may be more susceptible to weakened shells due to low pH environments caused by climate change.
Comparison of Hydric and Thermal Physiology in an Environmentally Diverse Clade of Caribbean Anoles
Authors: Alomar Nathalie , Bodensteiner Brooke L , Hernández-Rodríguez Isabela , Landestoy Miguel A , Domínguez-Guerrero Saúl F , Muñoz Martha M 
Published: May 2024

Synopsis
As the world becomes warmer and precipitation patterns less predictable, organisms will experience greater heat and water stress. It is crucial to understand the factors that predict variation in thermal and hydric physiology among species. This study focuses on investigating the relationships between thermal and hydric diversity and their environmental predictors in a clade of Hispaniolan anole lizards, which are part of a broader Caribbean adaptive radiation. This clade, the “cybotoid” anoles, occupies a wide range of thermal habitats (from sea level to several kilometers above it) and hydric habitats (such as xeric scrub, broadleaf forest, and pine forest), setting up the possibility for ecophysiological specialization among species. Among the thermal traits, only cold tolerance is correlated with environmental temperature, and none of our climate variables are correlated with hydric physiology. Nevertheless, we found a negative relationship between heat tolerance (critical thermal maximum) and evaporative water loss at higher temperatures, such that more heat-tolerant lizards are also more desiccation-tolerant at higher temperatures. This finding hints at shared thermal and hydric specialization at higher temperatures, underscoring the importance of considering the interactive effects of temperature and water balance in ecophysiological studies. While ecophysiological differentiation is a core feature of the anole adaptive radiation, our results suggest that close relatives in this lineage do not diverge in hydric physiology and only diverge partially in thermal physiology.
In-hospital thromboprophylaxis variation and the risk of venous thromboembolism after lung cancer surgery: a nationwide cohort study
Authors: Christensen Thomas Decker , Ording Anne Gulbech , Skjøth Flemming , Mørkved Amalie Lambert , Jakobsen Erik , Meldgaard Peter , Petersen Rene Horsleben , Søgaard Mette 
Published: May 2024

Abstract

OBJECTIVES
Venous thromboembolic event (VTE) is a severe complication in patients with lung cancer undergoing thoracic surgery. Nevertheless, because of insufficient evidence, there are no clear guidelines, and VTE prophylaxis practices vary widely. This nationwide cohort study was a comparative study investigating VTE risk in surgical departments that routinely administered in-hospital thromboprophylaxis with low-molecular-weight heparin compared to those that did not.

METHODS
We identified all patients with non-small-cell lung cancer (NSCLC) who underwent surgery in Denmark during 2010–2021. Thoracic surgery was exclusively performed in the 4 university hospitals. Three hospitals implemented in-hospital thromboprophylaxis as standard care since 2000, while the fourth adopted this practice in September 2016. VTE events were assessed at 6-month follow-up according to hospital and study period, using an inverse probability of treatment weighting approach.

RESULTS
We identified 9615 patients. During 6-month follow-up, a total of 190 VTE events were observed, resulting in a weighted rate of 4.5 events per 100 person-years and an absolute risk of 2.2%. There was no clear trend according to hospital site or use of in-hospital thromboprophylaxis with a 2.2% risk in the hospital not using thromboprophylaxis compared to 1.7–3.1% in those that did.

CONCLUSIONS
Use of in-hospital thromboprophylaxis did not affect the risk of VTE after surgery for NSCLC, suggesting that relying solely on in-hospital thromboprophylaxis may be insufficient to mitigate VTE risk in these patients. Further research is warranted to investigate the potential benefits of extended thromboprophylaxis in reducing VTE risk in selected NSCLC surgical patients.
Effect of sex and milk replacer with or without supplemental carnitine and arginine on growth characteristics, carcass, and meat quality of artificially reared low-birth weight pigs
Authors: Madsen Johannes G , Kreuzer Michael , Silacci Paolo , Bee Giuseppe 
Published: May 2024

Abstract
This study compared milk replacer either remaining unsupplemented (CON) or supplemented with 0.5 g L-carnitine plus 16.7 g L-arginine/kg (CarArg) and fed to 48 low-birth weight (L-BtW) artificially reared piglets (24 per group) from days 7 to 28 of age. Eight farrowing series were needed to complete the study. On day 28, the lightest piglets were slaughtered, and the heaviest pigs were weaned. The heaviest pigs were weaned on day 28 and offered free access to a starter (weaning to 25 kg body weight [BW]), grower (25 to 60 kg BW), and finisher diet (60 to 96 kg BW on day 170 of age). After euthanization on days 28 and 170, blood was sampled for assessment of serum metabolite and hormone concentrations, and the semitendinosus muscle (STM) was weighed, and later subjected to enzyme activity analysis and assessment of myofiber characteristics. In the 170-d-old pigs carcass and meat quality traits were assessed. Growth data were analyzed accordingtoatwo-way analysis of variance (ANOVA), with dietary treatment and farrowing series as fixed effects, while remaining data were analyzed with dietary treatment, sex, their interaction, and farrowing series as main factors. Dietary treatments affected (P ≤ 0.049) muscle enzyme activity at both day 28, with greater citrate synthase (CS) and LDH activities and lower HAD:CS ratio in STM light portion, and lower LDH:CS ratio in STM dark portion, and 170 of age with lower HAD:CS ratio. In the starter period, CarArg pigs had greater average daily gain (P = 0.021) and average daily feed intake (P = 0.010). At slaughter, these pigs had lower (P = 0.013) glucose and greater (P = 0.022) urea serum concentrations. However, supplementing the milk replacer with carnitine and arginine had no long-term effects on growth performance, carcass composition, and meat quality of L-BtW pigs. In addition, muscle morphology and myofiber-related properties remained unaffected by the supplementation.
Factors Related to Electrical Burn Injuries in Occupational and Nonoccupational Settings From 2010 to 2021 in Colombia: Cross-Sectional Design
Authors: Ayala Karen P , Pombo Luis M , Camero-Ramos Gabriel , Teherán Aníbal A , Ávila Albert A , Tocanchón Ginna P , Zuluaga-Ortíz Carol A 
Published: May 2024

Abstract
Burns affect 11 million people worldwide annually. Electrical burns (EBs) are renowned for inflicting extensive harm and long-term consequences that can lead to severe illnesses and fatalities. People in both occupational and nonoccupational settings may be exposed to EBs, leading to functional or anatomical consequences. We identified sociodemographic features related to EBs in both settings. A cross-sectional design, using an open dataset of electrical shock injuries that occurred in Colombia during the 2010-2021 period, was carried out. Sociodemographic features of people injured in occupational and nonoccupational settings were described in counts (%), incidence per 100 000 people (I0; 95% CI). To identify related factors (age-sex adjusted) with injuries in occupational and nonoccupational settings, we applied a binary logistic regression. Over the 12-year period, there were 1.274 EBs (I0: 2.47; 2.34-2.61), 287 in the occupational setting (I0: 1.35; 1.20-1.51), and 987 in the nonoccupational setting (I0: 3.25; 3.05-3.46). Age median was 31 years, and most cases were distributed in middle adulthood (52.8%), males (88.1%), high school/technician (42.8%), urban location (73.7%), weekdays (95.3%), and daytime hours (85.5%). Factors related to EBs in the occupational setting were males, middle adulthood, high school/technician, Thursday, and daytime hours. Other factors related to EBs in the nonoccupational setting were early childhood, primary school, urban location, and weekends. Both settings pose a risk of injuries to individuals. We have identified sociodemographic factors related to these injuries in both occupational and nonoccupational settings, which could aid in preventing damages and long-term complications, especially among vulnerable subgroups such as those determined above.
Reworking uneven geographical development: the spatial logic of China’s rural banking reforms
Authors: Yu Leqian , Yin Jie 
Published: May 2024

Abstract
Drawing on financial geography literature and the concept of state rescaling, this article investigates the state–finance nexus with an emphasis on state spatial reconfiguration. Through a historically and geographically informed political economic analysis, it argues that China’s state-led, market-oriented rural banking reforms are not merely the outcome of a deepening market logic within the financial administration of the rural sector. They are also crucial for state spatial reconfiguration to address uneven rural and urban development. The article calls for greater sensitivity towards relational spatiotemporality and uneven development to comprehend fully the spatiality of the state–finance nexus.
Hibernacula of bats in Mexico, the southernmost records of hibernation in North America
Authors: Ramos-H. Daniel , Marín Ganesh , Cafaggi Daniela , Sierra-Durán Cárol , Romero-Ruíz Aarón , Medellín Rodrigo A 
Published: May 2024

Abstract
Although Mexico holds the southernmost hibernating bats in North America, information on winter behavior and hibernacula microclimate use of temperate Mexican bats is limited. We studied hibernating bats at high altitudes (&gt;1,000 m a.s.l.) in northern and central Mexico during 5 consecutive winters. Our aims were to document and describe the hibernacula, winter behavior (such as abundance and roost pattern), and microclimates (estimated as adjacent substrate temperature) of cave-hibernating bats in Mexico. We found 78 hibernacula and 6,089 torpid bats of 10 vespertilionid species, increasing by over 50% the number of cave-hibernating bat species and quadrupling the number of hibernacula for Mexico. Hibernacula were at altitudes between 1,049 and 3,633 m a.s.l., located in 3 mountain ranges, mainly in oak and conifer forests. Myotis velifer was the most common species, followed by Corynorhinus townsendii and C. mexicanus. We recorded the adjacent substrate temperatures from 9 species totaling 1,106 torpid bats and found differences in microclimate use among the 3 most common species. In general, abundance of torpid bats in our region of study was similar to those in the western United States, with aggregations of tens to a few hundred individuals per cave, and was lower than in the eastern United States where a cave may hold thousands of individuals. Knowledge of bat hibernation is crucial for developing conservation and management strategies on current conditions while accommodating environmental changes and other threats such as emerging diseases.
Structure-specific nucleases in genome dynamics and strategies for targeting cancers
Authors: Sun Haitao , Luo Megan , Zhou Mian , Zheng Li , Li Hongzhi , Esworthy R Steven , Shen Binghui 
Published: May 2024

Abstract
Nucleases are a super family of enzymes that hydrolyze phosphodiester bonds present in genomes. They widely vary in substrates, causing differentiation in cleavage patterns and having a diversified role in maintaining genetic material. Through cellular evolution of prokaryotic to eukaryotic, nucleases become structure-specific in recognizing its own or foreign genomic DNA/RNA configurations as its substrates, including flaps, bubbles, and Holliday junctions. These special structural configurations are commonly found as intermediates in processes like DNA replication, repair, and recombination. The structure-specific nature and diversified functions make them essential to maintaining genome integrity and evolution in normal and cancer cells. In this article, we review their roles in various pathways, including Okazaki fragment maturation during DNA replication, end resection in homology-directed recombination repair of DNA double-strand breaks, DNA excision repair and apoptosis DNA fragmentation in response to exogenous DNA damage, and HIV life cycle. As the nucleases serve as key points for the DNA dynamics, cellular apoptosis, and cancer cell survival pathways, we discuss the efforts in the field in developing the therapeutic regimens, taking advantage of recently available knowledge of their diversified structures and functions.
Momentum-resolved EELS and CL study on 1D-plasmonic crystal prepared by FIB method
Authors: Yasuhara Akira , Shibata Masateru , Yamamoto Wakaba , Machfuudzoh Izzah , Yanagimoto Sotatsu , Sannomiya Takumi 
Published: May 2024

Abstract
We investigate a one-dimensional plasmonic crystal using momentum-resolved electron energy-loss spectroscopy (EELS) and cathodoluminescence (CL) techniques, which are complementary in terms of available optical information. The plasmonic crystal sample is fabricated from large aluminum grains through the focused ion beam method. This approach allows curving nanostructures with high crystallinity, providing platforms for detailed analysis of plasmonic nanostructures using both EELS and CL. The momentum-resolved EELS visualizes dispersion curves outside the light cone, confirming the existence of the surface plasmon polaritons and local modes, while the momentum-resolved CL mapping analysis identified these surface plasmon polaritons and local modes. Such synergetic approach of two electron-beam techniques offers full insights into both radiative and non-radiative optical properties in plasmonic or photonic structures.
Enteric Fever in Children: An Epidemiological and Clinical Review of Cases in Southeast Texas
Authors: Foughty Zachary C , Niles Denver T , Cruz Andrea T 
Published: May 2024

Abstract
Extensively drug-resistant (XDR) strains of Salmonella enterica serotype Typhi have emerged in Pakistan and Iraq. We report 13 children with enteric fever in Southeast Texas seen over 3.5 years, of whom 23.1% had XDR isolates.
Direct Observation of Trace Elements in Barium Titanate of Multilayer Ceramic Capacitors Using Atom Probe Tomography
Authors: Jang Kyuseon , Kim Mi-Yang , Jung Chanwon , Kim Se-Ho , Choi Daechul , Park Seong-Chan , Scheu Christina , Choi Pyuck-Pa 
Published: May 2024

Abstract
Accurately controlling trace additives in dielectric barium titanate (BaTiO3) layers is important for optimizing the performance of multilayer ceramic capacitors (MLCCs). However, characterizing the spatial distribution and local concentration of the additives, which strongly influence the MLCC performance, poses a significant challenge. Atom probe tomography (APT) is an ideal technique for obtaining this information, but the extremely low electrical conductivity and piezoelectricity of BaTiO3 render its analysis with existing sample preparation approaches difficult. In this study, we developed a new APT sample preparation method involving W coating and heat treatment to investigate the trace additives in the BaTiO3 layer of MLCCs. This method enables determination of the local concentration and distribution of all trace elements in the BaTiO3 layer, including additives and undesired impurities. The developed method is expected to pave the way for the further optimization and advancement of MLCC technology.
Integrated enhancer regulatory network by enhancer–promoter looping in gastric cancer
Authors: Zhu Tianhui , Okabe Atsushi , Usui Genki , Fujiki Ryoji , Komiyama Daichi , Huang Kie Kyon , Seki Motoaki , Fukuyo Masaki , Abe Hiroyuki , Ning Meng , Okada Tomoka , Minami Mizuki , Matsumoto Makoto , Fan Qin , Rahmutulla Bahityar , Hoshii Takayuki , Tan Patrick , Morikawa Teppei , Ushiku Tetsuo , Kaneda Atsushi 
Published: May 2024

Abstract
Enhancer cis-regulatory elements play critical roles in gene regulation at many stages of cell growth. Enhancers in cancer cells also regulate the transcription of oncogenes. In this study, we performed a comprehensive analysis of long-range chromatin interactions, histone modifications, chromatin accessibility and expression in two gastric cancer (GC) cell lines compared to normal gastric epithelial cells. We found that GC-specific enhancers marked by histone modifications can activate a population of genes, including some oncogenes, by interacting with their proximal promoters. In addition, motif analysis of enhancer–promoter interacting enhancers showed that GC-specific transcription factors are enriched. Among them, we found that MYB is crucial for GC cell growth and activated by the enhancer with an enhancer–promoter loop and TCF7 upregulation. Clinical GC samples showed epigenetic activation of enhancers at the MYB locus and significant upregulation of TCF7 and MYB, regardless of molecular GC subtype and clinicopathological factors. Single-cell RNA sequencing of gastric mucosa with intestinal metaplasia showed high expression of TCF7 and MYB in intestinal stem cells. When we inactivated the loop-forming enhancer at the MYB locus using CRISPR interference (dCas9-KRAB), GC cell growth was significantly inhibited. In conclusion, we identified MYB as an oncogene activated by a loop-forming enhancer and contributing to GC cell growth.
Outcomes and Predictors of Mortality in Patients With KPC-Kp Infections Treated With Meropenem Vaborbactam: An Observational Multicenter Study
Authors: Tumbarello Mario , Raffaelli Francesca , Giannella Maddalena , De Pascale Gennaro , Cascio Antonio , De Rosa Francesco Giuseppe , Cattelan Anna Maria , Oliva Alessandra , Saracino Annalisa , Bassetti Matteo , Mussini Cristina , Luzzati Roberto , Capone Alessandro , Signorini Liana , Bartoletti Michele , Sambo Margherita , Sarmati Loredana , Antinori Spinello , Mularoni Alessandra , Tascini Carlo , Corona Alberto , Pascale Renato , Rubino Raffaella , Corcione Silvia , Mazzitelli Maria , Giuliano Gabriele , Lovecchio Antonio , Bavaro Davide Fiore , Meschiari Marianna , Montagnani Francesca , Fabbiani Massimiliano , De Benedetto Ilaria , Antonelli Massimo , Venditti Mario , Viale Pierluigi 
Published: May 2024

Abstract

Background
Meropenem-vaborbactam is a recent and promising option for the treatment of KPC-producing Klebsiella pneumoniae (KPC-Kp) infections, including those resistant to ceftazidime-avibactam.

Methods
We conducted a retrospective analysis of observational data from 19 Italian hospitals on use and outcomes of patients treated with meropenem-vaborbactam for at least ≥24 hours for KPC-Kp infections. Crude and propensity-weighted multiple Cox regression models were performed to ascertain risk factors independently associated with 30-day mortality.

Results
The cohort included 342 adults with bloodstream infections (n = 172) and nonbacteremic infections (n = 170), of which 107 were lower respiratory tract infections, 30 were complicated urinary tract infections, and 33 were infections involving other sites. Most infections (62.3%) were managed with meropenem-vaborbactam monotherapy, or in combination with at least 1 other active drug (usually fosfomycin, tigecycline, or gentamicin) (37.7%). The 30-day mortality rate was 31.6% (108/342). In multiple Cox regression model, 30-day mortality was independently associated with septic shock at infection onset, Charlson comorbidity index ≥ 3, dialysis, concomitant COVID-19, and INCREMENT score ≥ 8. Administration of meropenem-vaborbactam within 48 hours from infection onset was a negative predictor of mortality. All predictors, except administration of meropenem-vaborbactam within 48 hours, remained significant when the multiple Cox regression model was repeated after adjustment for the propensity score for receipt of combination therapy.

Conclusions
Despite the limits of a retrospective study, the data derived from this multicenter cohort provide additional evidence on the efficacy of meropenem-vaborbactam in treating severe KPC-Kp infections, even when used as monotherapy.
Reference optical turbulence characteristics at the Large Solar Vacuum Telescope site
Authors: Shikhovtsev Artem Yu 
Published: May 2024

Abstract
Large ground-based solar telescopes are equipped with adaptive optics systems to correct wavefront distortions induced in the turbulent atmosphere. The design of the adaptive optics system strongly depends on the vertical profiles of the optical turbulence. In particular, the characteristics of the optical turbulence determine the design of tomographic adaptive optics systems, which provide image correction within a wide field of view. In the article, a new method to estimate reference optical turbulence characteristics from Era-5 reanalysis assimilated data is presented. This method is based on the dependence of the air refractive index structure constant $C_n^2$ on the vertical shears of wind speed as well as the outer scale of turbulence L0. The L0 parameter is estimated by minimization of the dispersion between the modeled and measured values of the refractive index structure constant $C_n^2$ within the surface layer. For the first time, parametrization coefficients and reference profiles of optical turbulence averaged for the period 1940–2022 are calculated for the Large Solar Vacuum Telescope (LSVT) site. The calculated optical turbulence profiles are representative; these profiles correspond to typical changes of the measured values of the Fried parameter, the isoplanatic angle, and the outer scale of turbulence at the LSVT site. The model turbulence profiles are verified taking into account the Shack–Hartmann wavefront sensor measurements at the LSVT. The higher accuracy of estimation of the optical turbulence characteristics makes it possible to refine parameters relevant to the LSVT adaptive optics system. The obtained results can be used in order to develop high-resolution solar adaptive optics technologies as applied to ground-based telescopes including those using the principles of atmospheric tomography.