bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthetic and Structural Studies on Copper 1 H‐[1,10]‐Phenanthrolin‐2‐one Coordination Complexes: Isolation of a Novel Intermediate During 1,10‐Phenanthroline Hydroxylation
Authors: Szpakolski Katherine B. , Latham Kay , Rix Colin J. , White Jonathan M. , Moubaraki Boujemaa , Murray Keith S. 
Published: December 2009

AbstractThe synthesis and crystal structure elucidation of a novel dinuclear heteroleptic copper(II) complex has led to an alternative mechanism in the formation of covalent hydrates. During further studies on the synthesis and properties of [Cu2(ophen)2] (1), a dinuclear complex of copper(I) with 1 H‐[1,10]‐phenanthrolin‐2‐one (Hophen), two intermediates/alternative products 2 and 3 were isolated. The dinuclear, antiferromagnetic complex [Cu2(ophen)2(phen)2]⋅(NO3)2⋅9H2O (3, phen=1,10‐phenanthroline) contains two five‐coordinate copper(II) ions, both with trigonal‐bipyramidal coordination, which are bridged together through deprotonated hydroxyl groups with a CuCu non‐bonding distance of 3.100 Å. Complex [Cu(phen)2(H2O)]⋅(NO3)2 (2) is a polymorph of a previously reported material. The occurrence of 2 and 3 has led us to propose a variation to the Gillard mechanism for the formation of covalent hydrates in bidentate N‐heterocycles in which the attacking nucleophile may be the deprotonated form of 2, [Cu(phen)2(OH)]−, rather than free OH−.
Secondary Interactions or Ligand Scrambling? Subtle Steric Effects Govern the Iridium(I) Coordination Chemistry of Phosphoramidite Ligands
Authors: Osswald Tina , Rüegger Heinz , Mezzetti Antonio 
Published: December 2009

AbstractThe like and unlike isomers of phosphoramidite (P*) ligands are found to react differently with iridium(I), which is a key to explaining the apparently inconsistent results obtained by us and other research groups in a variety of catalytic reactions. Thus, the unlike diastereoisomer (aR,S,S)‐[IrCl(cod)(1 a)] (2 a; cod=1,5‐cyclooctadiene, 1 a=(aR,S,S)‐(1,1′‐binaphthalene)‐2,2′‐diyl bis(1‐phenylethyl)phosphoramidite) forms, upon chloride abstraction, the monosubstituted complex (aR,S,S)‐[Ir(cod)(1,2‐η‐1 a,κP)]+ (3 a), which contains a chelating P* ligand that features an η2 interaction between a dangling phenyl group and iridium. Under analogous conditions, the like analogue (aR,R,R)‐1 a′ gives the disubstituted species (aR,R,R)‐[Ir(cod)(1 a′,κP)2]+ (4 a′) with monodentate P* ligands. The structure of 3 a was assessed by a combination of X‐ray and NMR spectroscopic studies, which indicate that it is the configuration of the binaphthol moiety (and not that of the dangling benzyl N groups) that determines the configuration of the complex. The effect of the relative configuration of the P* ligand on its iridium(I) coordination chemistry is discussed in the context of our preliminary catalytic results and of apparently random results obtained by other groups in the iridium(I)‐catalyzed asymmetric allylic alkylation of allylic acetates and in rhodium(I)‐catalyzed asymmetric cycloaddition reactions. Further studies with the unlike ligand (aS,R,R)‐(1,1′‐binaphthalene)‐2,2′‐diyl bis{[1‐(1‐naphthalene‐1‐yl)ethyl]phosphoramidite} (1 b) showed a yet different coordination mode, that is, the η4‐arene–metal interaction in (aS,R,R)‐[Ir(cod)(1,2,3,4‐η‐1 b,κP)]+ (3 b).
Catalytic Double Stereoinduction in Asymmetric Allylic Alkylation of Oxindoles
Authors: Trost Barry M. , Zhang Yong 
Published: December 2009

AbstractA highly regio‐, diastereo‐, and enantioselective allylic alkylation reaction of 3‐monosubstituted oxindoles catalyzed by molybdenum is described. The reaction is affected by the electronic and steric variations of the nucleophile. The use of appropriate N‐protecting group is particularly important for achieving high regio‐ and diastereoselectivity. Products from this reaction, containing vicinal quaternary‐tertiary stereogenic centers, are valuable synthetic intermediates and should find utility in alkaloid synthesis.
Optimierung einer rekombinanten mikrobiellen Transglutaminase
Authors: Büttner K. , Marx C. K. , Hertel T. C. , Pietzsch M. 
Published: December 2009

AbstractDie mikrobielle Transglutaminase aus Streptomyces mobaraensis wird bisher überwiegend in der Lebensmitteltechnologie angewendet, da durch die Vernetzung von Proteinen die Textureigenschaften z. B. von Wurstprodukten verändert werden können. Um das Enzym auch für andere Anwendungen und eine Optimierung verfügbar zu machen, wurde eine mikrobielle Transglutaminase aus S. mobaraensis kloniert und erstmals in löslicher Form in Escherichia coli exprimiert. Dies war die Voraussetzung, um das Enzym mittels Random Mutagenese optimieren zu können. Im mikrotiterplattenbasierenden Hochdurchsatz‐Screeningverfahren wurde nach thermostabilen Varianten gesucht. Unter 5500 Klonen wurden sechs thermostabile Mutanten identifiziert. Diese Enzymvarianten wurden bezüglich ihrer Thermostabilität und spezifischen Aktivität in Abhängigkeit von der Temperatur charakterisiert. Die beste thermostabile Variante besitzt eine einzige Mutation (S2P) und weist eine um 270 % erhöhte Halbwertszeit bei 60 °C auf und hat außerdem bei allen Temperaturen eine doppelt so hohe spezifische Aktivität als das rekombinante Wildtyp‐Enzym. Die Steigerung der spezifischen Aktivität ist vorteilhaft, da sich so mit der gleichen Enzymmenge die Reaktion in kürzerer Zeit durchführen lässt, bzw. die Enzymmenge reduziert werden kann.
Working Alliance Inventory‐Short Revised (WAI‐SR): psychometric properties in outpatients and inpatients
Authors: Munder Thomas , Wilmers Fabian , Leonhart Rainer , Linster Hans Wolfgang , Barth Jürgen 
Published: December 2009

AbstractThe Working Alliance Inventory‐Short Revised (WAI‐SR) is a recently refined measure of the therapeutic alliance that assesses three key aspects of the therapeutic alliance: (a) agreement on the tasks of therapy, (b) agreement on the goals of therapy and (c) development of an affective bond. The WAI‐SR demonstrated good psychometric properties in an initial validation in psychotherapy outpatients in the USA. The generalizability of these findings is limited because in some countries a substantial portion of individual psychotherapy is delivered in inpatient settings. This study investigated and compared the psychometric properties of the WAI‐SR in German outpatients (N = 88) and inpatients (N = 243). In both samples reliability (? ? 0.80) and convergent validity with the Helping Alliance Questionnaire were good (r > 0.64). Confirmatory factor analysis showed acceptable to good model fit for the proposed Bond‐Task‐Goal model in both samples. Multi‐group analysis demonstrated that the same constructs were measured across settings. Alliance ratings of outpatients and inpatients differed regarding the overlap of alliance components and the magnitude of the alliance ratings: The differentiation of the alliance components was poorer in inpatients and they reported lower alliances. Unique aspects of the alliance in inpatient treatment are discussed and a need for further research on the alliance in inpatient settings is pointed out. Overall, the WAI‐SR can be recommended for alliance assessment in both settings. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message

The Working Alliance Inventory‐Short Revised (WAI‐SR) is a 12‐item measure for the assessment of the therapeutic alliance.
The WAI‐SR is pantheoretic and captures three key alliance aspects: (a) agreement on the tasks of therapy, (b) agreement on the goals of therapy and (c) development of an affective bond.
This study demonstrates good psychometric properties of the WAI‐SR in outpatients and inpatients.
The WAI‐SR is ready to be used by psychotherapists interested in the therapeutic alliance, for supervision and process research.
Exploring therapeutic alliance in brief inpatient psychotherapy: a preliminary study
Authors: Blais Mark A. , Jacobo Michelle C. , Smith Steven R. 
Published: December 2009

AbstractBackground: Therapeutic alliance is one of the most widely investigated variables in psychotherapy research but few studies have explored its role in inpatient psychotherapy. Many factors likely contribute to the lack of inpatient alliance research including the short length of hospital stays, complexity of patient psychopathology and the burdensome quality of most alliance scales. This paper reports on the development and initial application of two comparable brief scales designed to capture patient and therapist alliance ratings. Method: Participants were 20 patients receiving supportive–expressive inpatient psychotherapy. The patients were predominantly depressed women. Baseline measures of distress, symptom severity and functioning were obtained at the first and third sessions. Measures of alliance were obtained at the second session. Results: The brief alliance scales demonstrated adequate internal consistency and the individual items had good adjusted item‐to‐scale correlations. Consistent with the broad alliance literature, we found that patients rated alliance higher than therapists, patient and therapist alliance ratings were not significantly correlated, and level of functioning was significantly associated with both patients and therapists' alliance ratings. The perceived depth of psychotherapy was also significantly associated with alliance. Unexpectedly, alliance ratings were also negatively associated with improvement during hospitalization. Conclusions: Overall, the study demonstrates both the feasibility and potential benefit of conducting inpatient psychotherapy research. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message:

This paper shows that inpatient psychotherapy can be studied and potentially improved through the application of brief targeted instruments.
Attachment and symptoms of psychopathology: early maladaptive schemas as a cognitive link?
Authors: Bosmans Guy , Braet Caroline , Van Vlierberghe Leen 
Published: December 2009

AbstractThis study investigated whether early maladaptive schemas can explain the relation between attachment anxiety and avoidance dimensions and symptoms of psychopathology. For this purpose, 289 Flemish, Dutch‐speaking, late adolescents participated on a questionnaire study. Using a non‐parametric re‐sampling approach, we investigated whether the association between attachment and psychopathology was mediated by early maladaptive schemas. Results indicate that the association between attachment anxiety and psychopathology is fully mediated by cognitions regarding rejection and disconnection and other‐directedness. The association between attachment avoidance and psychopathology is partly mediated by cognitions regarding rejection and disconnection. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message:

Our findings suggest that cognitive therapy might be useful in the treatment of attachment‐related psychopathology.
Our findings suggest that therapists should be wary for attachment‐related relapse.
Especially the cognitive schemas regarding expectations to be rejected or disconnected mediated the association between attachment anxiety and attachment avoidance dimensions and psychopathology.
Treatment of post‐traumatic stress disorder in children using cognitive behavioural writing therapy
Authors: Van der Oord Saskia , Lucassen S. , Van Emmerik A. A. P. , Emmelkamp P. M. G. 
Published: December 2009

AbstractObjectives: This study evaluated the effectiveness of Cognitive Behavioral Writing Therapy (CBWT) in 23 children (age 8–18 years) in the Netherlands, who experienced a range of single and recurrent traumatic experiences. CBWT uses exposure, cognitive restructuring and social sharing. Methods: At pre‐test, post‐test and follow‐up, post‐traumatic stress disorder (PTSD) symptoms, depressive symptoms, trauma‐related cognitions and general behavioural problems were assessed. Results: At post‐test there was a significant reduction of all symptoms, and this effect was maintained at 6 months follow‐up. The mean amount of treatment sessions needed was 5.5. Conclusions: This study shows that short‐term CBWT is a potentially effective intervention for clinically referred traumatized children. There is now a clear need of establishing the effectiveness of CBWT in a randomized, controlled trial. Practice implication: This first study indicates CBWT is a promising treatment, which can easily be used in clinical practice. Copyright © 2009 John Wiley & Sons, Ltd.Key practitioner massage:

CWBT can easily be implemented in clinical practice.
CWBT is suitable for a clinically representative group of children and adolescents with PTSD, who have experienced a wide range of single and recurrent traumatic experiences.
CWBT is a short‐term potentially effective treatment.
Artesunate plus amodiaquine combination therapy: reviewing the evidence
Authors: Adjei George O. , Kudzi William , Dodoo Alexander , Kurtzhals Jorgen A.L. 
Published: December 2009

AbstractThe combination of artesunate plus amodiaquine (AS/AQ) is, next to the fixed‐dose combination of artemether‐lumefantrine, the most widely adopted artemisinin combination therapy (ACT) regimen being used as first‐line therapy for uncomplicated malaria by National Malaria Control Programs in sub‐Saharan Africa. In contrast to other ACT regimens in current use, on which reviews of the available evidence on safety and efficacy have been periodically published, no, or few summaries of the extensive data available on the safety and efficacy of this widely used ACT are available in the published literature. The available evidence on AS/AQ indicates high efficacy of this combination in children, variable tolerability in adults, and an interaction between artesunate and amodiaquine that could have implications for medium term efficacy or safety of the combination. In this article, a review of the available evidence on the efficacy and safety on AS/AQ, with a particular focus on parameters that could have operational significance for malaria control in endemic areas, is presented and discussed. Drug Dev Res 71: 33–43, 2010. © 2009 Wiley‐Liss, Inc.
Malaria drugs: clues from malaria resistance genetics
Authors: Gurwitz David 
Published: December 2009

AbstractNew treatments for malaria are desperately needed. Malaria drug design can be assisted by knowledge on protective host genotypes. Individuals immune to severe malaria, being only mildly affected by the parasite infection, may carry alleles that provide protection from severe disease, which may provide clues for drug discovery. Lessons about protective genotypes have improved our understanding on the transmission and pathogenicity of the human immunodeficiency virus and have led to novel drug design. Similarly, clues from malaria protective genes may help in the design of new malaria therapies. Drug Dev Res 71: 1–3, 2010. © 2009 Wiley‐Liss, Inc.
Application of FAIMS to anabolic androgenic steroids in sport drug testing
Authors: Guddat Sven , Thevis Mario , Kapron James , Thomas Andreas , Schänzer Wilhelm 
Published: December 2009

AbstractMass spectrometric identification of anabolic androgenic steroids challenges standard doping‐control methods. To reveal a doping offence the presence of prohibited anabolic androgenic steroids at trace levels in the picogram‐per‐millilitre range must be confirmed as reliable. Human urine samples containing epitrenbolone, metandienone metabolite (17β ‐hydroxymethyl‐17α‐methyl‐18‐norandrost‐1,4,13‐trien‐3‐one), stanozolol, 16β‐hydroxystanozolol and 4β‐hydroxystanozolol were analysed using LC‐FAIMS‐MS/MS. These substances are prohibited in sport according to World Anti‐Doping Agency (WADA) regulations. Glucuronides were hydrolysed and prepared by liquid‐liquid extraction. Excellent recovery and precision were obtained for all compounds. Linear calibration results for epitrenbolone and metandienone metabolite were obtained and concentration information could be determined in the ranges of reliable response between 750–1200 and 100–600 pg/mL, respectively. Limits of detection were estimated at 25 pg/mL (stanozolol), 50 pg/mL (metandienone metabolite, 16β‐hydroxystanozolol), 100 pg/mL (4β‐hydroxystanozolol) and 500 pg/mL (epitrenbolone). The assay was applied to doping‐control samples. For all analytes, LC‐FAIMS‐MS/MS resulted in excellent interference removal, which effectively extends the post‐dose detection time. Copyright © 2009 John Wiley & Sons, Ltd.
Chemoselective Reagents for Covalent Capture and Display of Glycans in Microarrays
Authors: Cló Emiliano , Blixt Ola , Jensen Knud J. 
Published: December 2009

AbstractGlycobiology has made very significant progress in the past decades. However, further progress will significantly depend on the establishment of novel methods for miniaturized, high‐throughput analysis of glycan–protein interactions. Robust solid‐phase chemical tools and new, chemoselective reagents for biologically meaningful display of surface‐immobilized glycans are likely to play a key role. Here we present four new bifunctional linkers that allow highly chemoselective capture of unprotected glycans in solution to form glycan‐linker conjugates for direct construction of glycan microarrays (glycochips). The bifunctional linkers carry O‐linked aminooxy moieties, some with N‐substituents at one end and an amino group at the other. In addition, they contain a substituted benzene ring for UV traceability and improved purification of glycan‐linker conjugates. NMR spectroscopic studies in solution proved that N‐substituted aminooxy linkers provided model glycan‐linker conjugates with the β‐glucopyranoside configuration, i.e. the ring‐closed form required for biological recognition. Then an ensemble of glycan‐linker conjugates were assembled from mannobiose, lactose, and N‐acetyl‐lactosamine and used for covalent printing of glycan microarrays. The stability of oximes were studied both in solution and on‐chip. In solution, two of the linkers provided glycan‐linker conjugates with a remarkable stability at pH 4 or higher, on‐chip this relative stability was upheld. Two of the linkers, with different properties, are recommended for the glycobiology toolbox for the construction of glycan microarrays from unprotected glycans.
Suppression of tumorigenesis and metastasis of hepatocellular carcinoma by shRNA interference targeting on homeoprotein Six1
Authors: Ng Kevin T.P. , Lee Terence K.W. , Cheng Qiao , Wo Jana Y.H. , Sun Chris K.W. , Guo Dong‐Yong , Lim Zophia X. , Lo Chung‐Mau , Poon Ronnie T.P. , Fan Sheung‐Tat , Man Kwan 
Published: December 2009

AbstractWe previously demonstrated that the overexpression of homeoprotein Six1 in hepatocellular carcinoma (HCC) patients is associated with venous infiltration, advanced pathologic tumor metastasis (pTNM) stage and poor overall survival rate (Ng et al. Br J Cancer 2006;95:1050–5). In this study, short hairpin RNA (shRNA) interference approach was used to suppress the expression of Six1 in a metastatic HCC cell line MHCC97L. Stable transfectant MHCC97L‐shSix1 carrying Six1‐specific shRNA plasmid was established to downregulate Six1 expression to about 40% when compared with MHCC97L‐Control. In vitro functional assays demonstrated that the growth rate and proliferation ability of MHCC97L‐shSix1 cells were markedly decreased. Moreover, significant decrease of cell motility and invasiveness were observed in MHCC97L‐shSix1 cells. Data from in vivo xenograft tumorigenesis model demonstrated that the size of tumor in MHCC97L‐shSix1 group was dramatically reduced. Experimental and spontaneous metastasis models indicated that targeting Six1 suppression noticeably reduced the pulmonary metastasis in MHCC97L‐shSix1 group. To identify Six1‐regulated targets, cDNA microarray was employed to compare the expression profiles of MHCC97L‐Control and MHCC97L‐shSix1 cells. Twenty‐eight downregulated and 24 upregulated genes with known functions were identified in MHCC97L‐shSix1. The functions of these target genes are involved in diverse biological activities. Our data suggest that Six1 may be involved in regulation of proliferation and invasiveness of HCC; thus targeting suppression of Six1 is a viable option for treating HCC patients.
The precancerous effect of emitted cooking oil fumes on precursor lesions of cervical cancer
Authors: Lee Chien‐Hung , Yang Sheau‐Fang , Peng Chiung‐Yu , Li Ruei‐Nian , Chen Yu‐Chieh , Chan Te‐Fu , Tsai Eing‐Mei , Kuo Fu‐Chen , Huang Joh‐Jong , Tsai Hsiu‐Ting , Hung Yu‐Hsiu , Huang Hsiao‐Ling , Tsai Sharon , Wu Ming‐Tsang 
Published: December 2009

AbstractAlthough cooking emission from high‐temperature frying has been deemed a Group 2A carcinogen by the International Agency for Research on Cancer, little is known about its impact on cervical tumorigenesis. To investigate the precancerous consequence of cooking oil fumes on cervical intraepithelial neoplasm (CIN), a community‐based case‐control study, which takes all known risk factors into consideration, was conducted in Taiwan. From 2003 to 2008, in a Pap smear screening and biopsy examination network, 206 pathology‐verified women with inflammations/atypical squamous cells of undetermined significance or CIN grade‐1 (CIN1) and 73 with CIN2−3 (defined as low‐grade squamous intraepithelial lesions (LGSIL) and high‐grade squamous intraepithelial lesions (HGSIL), respectively); and 1,200 area‐and‐age‐matched controls with negative cytology were recruited. Multinomial logistic regression was applied in the multivariate analysis to determine the likelihood of contracting LGSIL or HGSIL. The risks of the two lesions increased with the increase of carcinogenic high‐risk human papillomavirus DNA load, with a clear dose‐response relationship. Chefs were observed to experience a 7.9‐fold elevated HGSIL risk. Kitchens with poor fume ventilation during the main cooking life‐stage correlated to a 3.7‐fold risk of HGSIL, but not for LGSIL. More than 1 hr of daily cooking in kitchens with poor fume conditions appeared to confer an 8.4‐fold HGSIL risk, with an 8.3‐fold heterogeneously higher odds ratio than that (aOR = 1.0) for LGSIL. Similar risk pattern has been reproduced among never‐smoking women. Our findings demonstrate the association between indoor exposure to cooking fumes from heated oil and the late development of cervical precancerous lesions. This final conclusion needs to be verified by future research.
Calibration of a distributed irrigation water management model using remotely sensed evapotranspiration rates and groundwater heads
Authors: Jhorar Raj Kumar , Smit A.A.M.F.R. , Bastiaanssen W.G.M. , Roest C.W.J. 
Published: December 2009

AbstractParameters of the distributed irrigation water management model FRAME are determined by an inverse method using evapotranspiration (ET) rates estimated from the SEBAL remote sensing procedure and in situ measurement of groundwater heads. The model simulates canal and on‐farm water management as well as regional groundwater flow. The calibration is achieved in two phases. The data on ET were introduced with the primary intent of improving predictions of ET through better estimated soil hydraulic parameters. During the first phase, soil hydraulic parameters sensitive to ET were optimized. As per the canal running schedule in the study area, the daily values of ET data were synthesized into 16 time periods with 15 periods each of 24 days and one period of 5 days. Use of cumulative (annual basis) ET data results in better estimates of soil hydraulic parameters as compared to temporal (24‐day period basis) ET data due to possible errors in other input data. During the second phase of calibration, aquifer drainable porosity and maximum allowable groundwater extraction were optimized against groundwater heads for five years. The calibration was very successful in about 70% of the study area with a coefficient of correlation between simulated and observed groundwater levels of more than 80%. Subsequently the model is validated against groundwater heads for nine years. Copyright © 2009 John Wiley & Sons, Ltd.