bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effectiveness of evidence‐based healthcare educational interventions on healthcare professionals' knowledge, skills, attitudes, professional practice and healthcare outcomes: Systematic review and meta‐analysis
Authors: Hill James , Gratton Nikki , Kulkarni Amit , Hamer Oliver , Harrison Joanna , Harris Catherine , Chesters Jen , Duddy Elaine , Collins Lindsey , Clegg Andrew 
Published: May 2024

AbstractObjectiveThe primary aim of this systematic review is to assess the effectiveness of evidence‐based healthcare (EBHC) educational interventions on healthcare professionals' knowledge, skills, attitudes, behaviour of EBHC, clinical process and care outcomes. A secondary aim of the review is to assess the effects of important pedagogical moderating factors for EBHC educational interventions.MethodThis systematic review used a forward and backward citation search strategy on the Web of Science platform (date of inception to 28 April 2023). Only randomised controlled trials (RCTs) and cluster RCTs which compared EBHC educational interventions for healthcare professionals were included. A random effects meta‐analysis was undertaken for EBHC compared with an active and nonactive control for all outcomes.ResultsSixty‐one RCTs were identified which included a total of 5208 healthcare professionals. There was a large effect for EBHC educational interventions compared with waiting list/no treatment/sham control on knowledge (SMD, 2.69; 95% CI, 1.26–4.14, GRADE Low), skills (SMD, 0.88; 95% CI, 0.25–1.73, Very Low Certainty), attitude (SMD, 0.81; 95% CI, 0.16–1.47, Very Low Certainty) and behaviour of EBHC (SMD, 0.82; 95% CI, 0.25–1.40, Very Low Certainty). Over time the effect of EBHC educational interventions substantially decreased with no evidence of effect at 6 months for any outcome except behaviour (SMD,1.72; 95% CI, 0.74–2.71, Low Certainty). There was some evidence that blended learning, active learning and consistency in the individual delivering the intervention may be important positive moderating factors.ConclusionThese findings suggest that EBHC educational interventions may have a large short‐term effect on improving healthcare professionals' knowledge, skills, attitude and behaviour of EBHC. These effects may be longer‐lasting regarding EBHC behaviour. In terms of pedagogy, blended learning, active learning, and consistency of the individual delivering the intervention may be important positive moderating factors.
A consulting room with a view: Psychotherapy and the ecological context
Authors: Tramonti Francesco , Ferrante Benedetta , Palmer Hugh 
Published: May 2024

AbstractBackground:The field of psychotherapy is witnessing a lively debate today. The literature on the efficacy of psychological interventions has grown exponentially, but the assumptions of the prevailing research paradigms have been criticised from many points of view. One of the main concerns is that of a too often simplistic conception of psychotherapy, viewed as a set of specific methods for the treatment of point‐like problems or symptoms.Literature Review on Mental Health and the Ecological ContextInstead, emerging perspectives are trying to promote a more complex view on psychotherapy, as a process based on the therapeutic potential of relationships, which takes place in relevant social and cultural contexts. Even the ecological context is taken in greater consideration, as growing evidence exists about the detrimental effects of ecological threats—such as climate change and other environmental challenges—on mental health.ConclusionsPsychotherapists and psychotherapy associations are now paying due attention to such issues, as well as to social justice and other dimensions that no longer can be thought of as mere elements of the scenario in which psychotherapy is practiced. Rather they are dimensions that exert a strong influence on psychological well‐being, and thus must be properly acknowledged and assessed to connect clinical work with the communities and ecological contexts in which people live.
AtRKD5 inhibits the parthenogenic potential mediated by AtBBM
Authors: Liu Qiyan , Han Dongfen , Cheng Denghu , Chen Jinfan , Tian Shujuan , Wang Jiafa , Liu Man , Yuan Li 
Published: May 2024

ABSTRACTParthenogenesis, the development of unfertilized egg cells into embryos, is a key component of apomixis. AtBBM (BABY BOOM), a crucial regulator of embryogenesis in Arabidopsis, possesses the capacity to shift nutritional growth toward reproductive growth. However, the mechanisms underlying AtBBM‐induced parthenogenesis remain largely unexplored in dicot plants. Our findings revealed that in order to uphold the order of sexual reproduction, the embryo‐specific promoter activity of AtBBM as well as repressors that inhibit its expression in egg cells combine to limiting its ability to induce parthenogenesis. Notably, AtRKD5, a RWP‐RK domain‐containing (RKD) transcription factor, binds to the 3′ end of AtBBM and is identified as one of the inhibitory factors for AtBBM expression in the egg cell. In the atrkd5 mutant, we successfully achieved enhanced ectopic expression of AtBBM in egg cells, resulting in the generation of haploid offspring via parthenogenesis at a rate of 0.28%. Furthermore, by introducing chimeric Arabidopsis and rice BBM genes into the egg cell, we achieved a significant 4.6‐fold enhancement in haploid induction through the atdmp8/9 mutant. These findings lay a strong foundation for further exploration of the BBM‐mediated parthenogenesis mechanism and the improvement of haploid breeding efficiency mediated by the dmp8/9 mutant.
First record of Cebidicola armatus (Phthiraptera: Trichodectidae) on the Brachyteles hypoxanthus (Primate: Atelidae) in Brazil
Authors: Testa Mikaelly Frasson , da Silva Breno Torres , Class Camila Souza Carvalho , Pissinati Alcides , Paiva Larissa Calais , Silva Hallana Couto e , Tabacow Fernanda Pedreira , de Melo Fabiano Rodrigues , Strier Karen B. , da Silva Barbosa Alynne 
Published: May 2024

AbstractThis study reports for the first time the infestation of Cebidicola armatus in Brachyteles hypoxanthus. In total, 14 lice were recovered of an individual in Minas Gerais, Brazil. These were subjected to a microscopic analysis and the identification of C. armatus was carried out through its very characteristic morphology.
High‐intensity intermittent inspiratory and abdominal muscle combined training in respiratory, swallowing and systemic muscles of healthy adults
Authors: Zhang Linlin , Okazaki Tatsuma , Ebihara Satoru , Izumi Shin‐Ichi 
Published: May 2024

AbstractBackgroundLow‐intensity continuous inspiratory muscle training improves its strength. The abdominal muscles are the main expiratory muscles, and their training may improve expiratory muscle strength. Respiratory muscle strength regulates coughing effectiveness, which is critical for pneumonia management. In older people, risk factors for the development of pneumonia were respiratory muscle weakness and swallowing impairment. Currently, the impact of high‐intensity intermittent inspiratory and abdominal muscle combined training on the respiratory, swallowing, and systemic muscles is unclear.ObjectiveWe aimed to explore the effects of high‐intensity inspiratory muscle training combined with or without abdominal muscle training on respiratory muscle strength as well as the strength, mass, and performance of swallowing and systemic muscles.MethodsTwenty‐eight healthy adults were divided into two groups. Participants performed high‐intensity intermittent inspiratory muscle single or its combination with abdominal muscle training for 4 weeks. Respiratory muscle strength, swallowing muscle strength and mass, systemic muscle strength, mass and performance were measured at baseline, Week 2 and Week 4.ResultsBoth groups showed greater maximal respiratory pressures at Week 2 and Week 4 than baseline. Both groups showed improved tongue pressure and geniohyoid muscle thickness at Week 4. In addition, the combined training group improved body trunk muscle mass, handgrip strength and five‐time chair stand test, whereas the single training group did not.ConclusionThis study revealed that high‐intensity inspiratory muscle training improved inspiratory muscle strength and swallowing muscle strength and mass. Moreover, inspiratory and abdominal muscle combined training showed an additional benefit of improving systemic muscle strength, mass and performance.Clinical Trial Registration NumberUMIN000046724; https://upload.umin.ac.jp/cgi‐open‐bin/ctr/index.cgi?ctrno=UMIN000046724.
In‐hospital mortality in dogs with protein‐losing enteropathy and associated risk factors
Authors: Hawes Connor , Kathrani Aarti 
Published: May 2024

AbstractBackgroundRisk factors associated with negative outcomes in dogs with protein‐losing enteropathy (PLE) are well documented. However, mortality before hospital discharge and associated risk factors are not well described.Hypothesis/ObjectivesReport the percentage of dogs with PLE that do not survive to hospital discharge and identify associated risk factors.AnimalsOne‐hundred and seven dogs presented to a referral hospital and diagnosed with PLE caused by inflammatory enteritis, intestinal lymphangiectasia or both.MethodsRetrospective cross‐sectional study assessing hospital records. Data on in‐hospital mortality and cause were assessed, and presenting signs, treatments prescribed, neutrophil count, lymphocyte count, serum albumin, globulin, and C‐reactive protein (CRP) concentrations, and histopathologic findings were compared between survivors and non‐survivors.ResultsIn‐hospital mortality was 21.5% with the most common causes including financial limitations, failure to improve and aspiration pneumonia. Factors associated with mortality during hospitalization included longer duration of hospitalization (P = .04), longer duration of clinical signs (P = .02) and an increase in serum CRP concentration after 1–3 days of in‐hospital treatment (P = .02). Higher mortality was identified in Pugs (odds ratio [OR], 4.93; 95% confidence interval [CI], 1.41–17.2; P = .01) and was a result of presumptive aspiration pneumonia in 5/6 of these dogs.Conclusions and Clinical ImportanceProtein‐losing enteropathy in dogs has substantial mortality during hospitalization. Monitoring for improvement in CRP concentration after treatment during hospitalization may help predict survival to discharge. Pugs have increased in‐hospital mortality because of aspiration pneumonia; measures to prevent, recognize, and promptly treat this complication may improve outcomes in this breed.
LncRNA EIF3J‐DT promotes chemoresistance in oral squamous cell carcinoma
Authors: Kim Seon Hyun , Kim Hye Jung , Kim Yeong Joo , Kim Yun Hak , Park Hae Ryoun 
Published: May 2024

AbstractObjectivesThis study aimed to screen oral squamous cell carcinoma (OSCC) diagnostic and prognostic candidates and investigate the potential functions and mechanisms of candidates in the chemoresistance of OSCC cell lines.Materials and MethodsDifferential expression profiling of lncRNA was performed in a large cohort of OSCC patients from the Cancer Genome Atlas database to identify OSCC diagnostic and prognostic candidates. Taxol resistance in OSCC cell lines was analyzed using MTT assay. OSCC cell lines transfected with EIF3J‐DT pcDNA or siRNA were used to determine its regulatory effects on apoptosis, cell cycle distribution and autophagy using flow cytometry and western blot.ResultsWe identified EIF3J‐DT as a candidate for OSCC diagnosis and prognosis. The expression level of EIF3J‐DT in OSCC cell lines correlates with taxol resistance. EIF3J‐DT silencing attenuated taxol resistance, and EIF3J‐DT overexpression enhanced taxol resistance in OSCC cell lines. Silencing of EIF3J‐DT reduced taxol resistance by inducing apoptosis, cell cycle arrest, and ATG14‐mediated autophagy inhibition in OSCC cell lines.ConclusionsWe found that EIF3J‐DT induced chemoresistance by regulating apoptosis, cell cycle, and autophagy in OSCC cell lines, which EIF3J‐DT might provide a novel therapeutic approach for OSCC as well as a diagnostic and prognostic factor.
Preface: Integrable systems and their applications, celebrating the 70th birthday of Athanassios S. Fokas
Authors: Ablowitz Mark J. , He Jingsong , Pelloni Beatrice 
Published: May 2024

AbstractThis special issue of Studies in Applied Mathematics is dedicated to the 70th birthday of Professor Thanasis Fokas. It contains a selection of papers that all use in some ways the ground‐breaking mathematical ideas and techniques introduced by Thanasis over his long and exceptionally prolific career.
Wafer Edge Metrology and Inspection Technique Using Curved-Edge Diffractive Fringe Pattern Analysis
Authors: Lu Kuan , Wang Zhikun , Chun Heebum , Lee ChaBum 
Published: May 2024

Abstract
This paper introduces a novel wafer-edge quality inspection method based on analysis of curved-edge diffractive fringe patterns, which occur when light is incident and diffracts around the wafer edge. The proposed method aims to identify various defect modes at the wafer edges, including particles, chipping, scratches, thin-film deposition, and hybrid defect cases. The diffraction patterns formed behind the wafer edge are influenced by various factors, including the edge geometry, topography, and the presence of defects. In this study, edge diffractive fringe patterns were obtained from two approaches: (1) a single photodiode collected curved-edge interferometric fringe patterns by scanning the wafer edge and (2) an imaging device coupled with an objective lens captured the fringe image. The first approach allowed the wafer apex characterization, while the second approach enabled simultaneous localization and characterization of wafer quality along two bevels and apex directions. The collected fringe patterns were analyzed by both statistical feature extraction and wavelet transform; corresponding features were also evaluated through logarithm approximation. In sum, both proposed wafer-edge inspection methods can effectively characterize various wafer-edge defect modes. Their potential lies in their applicability to online wafer metrology and inspection applications, thereby contributing to the advancement of wafer manufacturing processes.
Repeatability in Measurements and Control Settings of a Small Francis Turbine Test Rig
Authors: Kumar Sandeep , Gandhi Bhupendra K. , Cervantes Michel J. 
Published: May 2024

Abstract
A reduced scaled-down hydraulic turbine test setup has been designed. The test setup is made to investigate the mitigation of pressure pulsations like the rotating vortex rope (RVR) and its formation and problems related to prototype operation. The setup arrangements and instrumentations follow mostly the International Electrotechnical Commission (IEC) standards. This paper presents the runner hub modification, calibration procedure, repeatability and systematic uncertainty estimation, and measurement methods per the guidelines in IEC 60193 and IEC 60041. The calibration and regression errors of all instruments are presented. A labview program for open-loop operation at a constant head and closed-loop operation at constant discharge is also presented. Finally, the systematic and random uncertainty of hydraulic efficiency is discussed.
Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles in
Magnetospirillum magneticum
AMB-1
Authors: Bickley Carson D. , Wan Juan , Komeili Arash 
Published: May 2024

ABSTRACT

Magnetotactic bacteria are a diverse group of microbes that use magnetic particles housed within intracellular lipid-bounded magnetosome organelles to guide navigation along geomagnetic fields. The development of magnetosomes and their magnetic crystals in
Magnetospirillum magneticum
AMB-1 requires the coordinated action of numerous proteins. Most proteins are thought to localize to magnetosomes during the initial stages of organelle biogenesis, regardless of environmental conditions. However, the magnetite-shaping protein Mms6 is only found in magnetosomes that contain magnetic particles, suggesting that it might conditionally localize after the formation of magnetosome membranes. The mechanisms for this unusual mode of localization to magnetosomes are unclear. Here, using pulse-chase labeling, we show that Mms6 translated under non-biomineralization conditions translocates to pre-formed magnetosomes when cells are shifted to biomineralizing conditions. Genes essential for magnetite production, namely
mamE, mamM,
and
mamO,
are necessary for Mms6 localization, whereas
mamN
inhibits Mms6 localization. MamD localization was also investigated and found to be controlled by similar cellular factors. The membrane localization of Mms6 is dependent on a glycine-leucine repeat region, while the N-terminal domain of Mms6 is necessary for retention in the cytosol and impacts conditional localization to magnetosomes. The N-terminal domain is also sufficient to impart conditional magnetosome localization to MmsF, altering its native constitutive magnetosome localization. Our work illuminates an alternative mode of protein localization to magnetosomes in which Mms6 and MamD are excluded from magnetosomes by MamN until biomineralization initiates, whereupon they translocate into magnetosome membranes to control the development of growing magnetite crystals.

IMPORTANCE
Magnetotactic bacteria (MTB) are a diverse group of bacteria that form magnetic nanoparticles surrounded by membranous organelles. MTB are widespread and serve as a model for bacterial organelle formation and biomineralization. Magnetosomes require a specific cohort of proteins to enable magnetite formation, but how those proteins are localized to magnetosome membranes is unclear. Here, we investigate protein localization using pulse-chase microscopy and find a system of protein coordination dependent on biomineralization-permissible conditions. In addition, our findings highlight a protein domain that alters the localization behavior of magnetosome proteins. Utilization of this protein domain may provide a synthetic route for conditional functionalization of magnetosomes for biotechnological applications.
Complete mitochondrial genome of the introduced Indian walking stick
Carausius morosus
(Lonchodidae, Insecta) from California
Authors: Clarke Aiden , Trujillo Alice , Mandujano Andres , Fernandez Angelica G. , Chambers Aniyah , Ruiz Nunez Areli , Contreras Audri , Cuevas Benny , Collins Caitlin , Trujillo Christian B. , Dominguez-Trejo Claudia L. , Bustamante Danilo E. , Pantoja-Garcia Eduardo , Anguiano Elizabeth , Alcaraz Emily D. , Rodriguez Felipe , Mora Flavio C. , Tinoco Rivera Froylan , Cabrera Luis Gladys , Nava Hailey B. , Huynh Henry N. , Diaz Javier C. , Hughey Jeffery R. , Do Jenny , Sevilla Jeriel S. , Llaja Jessica C. , Lopez Jessica , Rosas Jesus , Perez Jhordy , Oyola Johann E. , Carrion Jois V. , Black Joni J. , Chavez Jorge F. , Barboza José I. , Rodriguez Cortes Juan Pablo , Barrett Konnor L. , Prescott Lacey E. , Alvarez Layla , Merino Juarez Lizbet , Velasquez-Moreno Maria J. , Marquez-Gonzalez Mariah I. , Aguirre Linares Mariana , Chavez-Huigo Maricela , Calderon Martha S. , Brambila Mateo , Villa Maximiliano , Windham Mia J. , Perez Michael , Trujillo Natalie , Chenevert Pearl , Lewis Phoebe , Guiop Pilar , Mubarz Reema Y. , Garcia Velazquez Roberto , Ayala-Tocto Rosmery Y. , Santos Samantha , Fernandez-Güimac Samia L. J. , Zalasar Sandra R. , Aguilar-Trauco Smith E. , Duran Soledad , Solis Stephanie , Meza Steven L. , Al-Zuhairi Taym , Padilla Victor M. , Olano Yadhira M. , Alfaro Maldonado Yareli ,   on behalf of Hartnell College Genomics Group
Published: May 2024

ABSTRACT

We present the complete mitochondrial genome of
Carausius morosus
from Salinas, CA. The mitochondrial genome of
C. morosus
is circular, AT rich (78.1%), and 16,671 bp in length. It consists of 13 protein-coding, 22 transfer RNA, and 2 ribosomal RNA genes and is identical in gene content to
Carausius
sp.
Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform from
Bacillus thuringiensis
Authors: Liu Lu , Luo Dehua , Zhang Yongji , Liu Dingqi , Yin Kang , Tang Qing , Chou Shan-Ho , He Jin 
Published: May 2024

ABSTRACT

A riboswitch generally regulates the expression of its downstream genes through conformational change in its expression platform (EP) upon ligand binding. The cyclic diguanosine monophosphate (c-di-GMP) class I riboswitch Bc1 is widespread and conserved among
Bacillus cereus
group species. In this study, we revealed that Bc1 has a long EP with two typical ρ-independent terminator sequences 28 bp apart. The upstream terminator T1 is dominant
in vitro
, while downstream terminator T2 is more efficient
in vivo
. Through mutation analysis, we elucidated that Bc1 exerts a rare and incoherent “transcription-translation” dual regulation with T2 playing a crucial role. However, we found that Bc1 did not respond to c-di-GMP under
in vitro
transcription conditions, and the expressions of downstream genes did not change with fluctuation in intracellular c-di-GMP concentration. To explore this puzzle, we conducted SHAPE-MaP and confirmed the interaction of Bc1 with c-di-GMP. This shows that as c-di-GMP concentration increases, T1 unfolds but T2 remains almost intact and functional. The presence of T2 masks the effect of T1 unwinding, resulting in no response of Bc1 to c-di-GMP. The high Shannon entropy values of EP region imply the potential alternative structures of Bc1. We also found that zinc uptake regulator can specifically bind to the dual terminator coding sequence and slightly trigger the response of Bc1 to c-di-GMP. This work will shed light on the dual-regulation riboswitch and enrich our understanding of the RNA world.

IMPORTANCE
In nature, riboswitches are involved in a variety of metabolic regulation, most of which preferentially regulate transcription termination or translation initiation of downstream genes in specific ways. Alternatively, the same or different riboswitches can exist in tandem to enhance regulatory effects or respond to multiple ligands. However, many putative conserved riboswitches have not yet been experimentally validated. Here, we found that the c-di-GMP riboswitch Bc1 with a long EP could form a dual terminator and exhibit non-canonical and incoherent “transcription-translation” dual regulation. Besides, zinc uptake regulator specifically bound to the coding sequence of the Bc1 EP and slightly mediated the action of Bc1. The application of SHAPE-MaP to the dual regulation mechanism of Bc1 may establish the foundation for future studies of such complex untranslated regions in other bacterial genomes.
Impact of Deposition Parameters on the Optical Properties of Fe2O3 Thin Films Prepared by SILAR Method
Authors: Al-Noamny Abbas Hadi Abbas , Alshamarti Hussein. A. , Mohammed Kahtan A. , Alkhayat Adel H. Omran , Sharma Shubham 
Published: May 2024

In order to explore the structures, morphology, and optical features of Fe2O3 nanofilms, the successive ionic layer adsorption and reactions (SILARs) technique was utilized to deposit the nanofilms onto glassy slides at varying concentrations of the precursor. Three precursor concentrations (0.025, 0.05 and 0.075) M were used. Analysis using X-ray diffraction (XRD) presented the crystalline structure of the Fe2O3 thin films. Field emission-scanning electron microscopy (FE-SEM) was used to evaluate the morphological features of the films. The results demonstrate that the surfaces of the films are nonporous and that there is a good distribution for spherical nanosize particles. The number of cycles is another important parameter that has been tested and its effect on the optical properties of prepared films was evaluated. The optical properties like optical absorption and energy gaps are affected directionally by changing the concentration and cycle numbers during the preparation. The latest objective of our study is to determine the role of precursor concentrations and deposition cycles in preparing thin films that have electronic applications such as sensors and solar cells.