bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise and Pain Neuroscience Education for Patients With Chronic Pain After Total Knee Arthroplasty
Authors: Larsen Jesper B. , Skou Søren T. , Laursen Mogens , Bruun Niels Henrik , Arendt-Nielsen Lars , Madeleine Pascal 
Published: May 2024

ImportanceUp to 20% of patients develop chronic pain after total knee arthroplasty (TKA), yet there is a scarcity of effective interventions for this population.ObjectiveTo evaluate whether neuromuscular exercise and pain neuroscience education were superior to pain neuroscience education alone for patients with chronic pain after TKA.Design, Setting, and ParticipantsA superiority randomized clinical trial was conducted at 3 outpatient clinics at Aalborg University Hospital in Denmark. Participants with moderate-to-severe average daily pain intensity and no signs of prosthesis failure at least 1 year after primary TKA were included. Participant recruitment was initiated on April 12, 2019, and completed on October 31, 2022. The 12-month follow-up was completed on March 21, 2023.InterventionsThe study included 24 sessions of supervised neuromuscular exercise (2 sessions per week for 12 weeks) and 2 total sessions of pain neuroscience education (6 weeks between each session) or the same pain neuroscience education sessions alone. The interventions were delivered in groups of 2 to 4 participants.Main Outcomes and MeasuresThe primary outcome was change from baseline to 12 months using the mean score of the Knee Injury and Osteoarthritis Outcome Score, covering the 4 subscales pain, symptoms, activity of daily living, and knee-related quality of life (KOOS4; scores range from 0 to 100, with higher scores indicating better outcomes). The outcome assessors and statistician were blinded. All randomized participants were included in the intention-to-treat analysis.ResultsAmong the 69 participants (median age, 67.2 years [IQR, 61.2-71.9 years]; 40 female [58%]) included in the study, 36 were randomly assigned to the neuromuscular exercise and pain neuroscience education group, and 33 to the pain neuroscience education–alone group. The intention-to-treat analysis showed no between-group difference in change from baseline to 12 months for the KOOS4 (7.46 [95% CI, 3.04-11.89] vs 8.65 [95% CI, 4.67-12.63] points; mean difference, −1.33 [95% CI, −7.59 to 4.92]; P = .68). Among the 46 participants who participated in the 12-month assessment in the 2 groups, 16 (34.8%) experienced a clinically important improvement (a difference of ≥10 points on the KOOS4) with no between-group difference. No serious adverse events were observed.Conclusions and RelevanceIn this randomized clinical trial, the results demonstrated that neuromuscular exercises and pain neuroscience education were not superior to pain neuroscience education alone in participants with chronic pain after TKA. Approximately one-third of the participants, regardless of intervention, experienced clinically important improvements. Future studies should investigate which patient characteristics indicate a favorable response to exercises and/or pain neuroscience education.Trial RegistrationClinicalTrials.gov Identifier: NCT03886259
Data from NAATP build case for value of extended residential treatment
Authors: Enos Gary 
Published: May 2024

On a conference day that National Association of Addiction Treatment Providers (NAATP) CEO Marvin Ventrell dubbed “science day,” one statistic stood out to qualify as the data point of the day. The chief science officer at Cumberland Heights Foundation, an active participant in NAATP's groundbreaking Foundation for Recovery Science and Education (FoRSE) outcomes project, cited 2023 readmission statistics across all levels of care in the Nashville‐based organization. At one‐year post‐discharge, patients whose treatment stay had been shorter than 30 days had a readmission rate of 25%. For those whose stay had been longer than 30 days, the readmission rate was only 3%.
Primate‐Specific DAZ Regulates Translation of Cell Proliferation‐Related mRNAs and is Essential for Maintenance of Spermatogonia
Authors: Ou Ningjing , Wang Yuci , Xu Shuai , Luo Jiaqiang , Zhang Chenwang , Zhang Yangyi , Shi Xiaoyan , Xiong Minggang , Zhao Liangyu , Ji Zhiyong , Zhang Yuxiang , Zhao Jingpeng , Bai Haowei , Tian Ruhui , Li Peng , Zhi Erlei , Huang Yuhua , Chen Wei , Wang Ruiqi , Jin Yuxuan , Wang Dian , Li Zheng , Chen Hao , Yao Chencheng 
Published: May 2024

AbstractPrimate‐specific DAZ (deleted in azoospermia) has evolved in the azoospermia factor c (AZFc) locus on the Y chromosome. Loss of DAZ is associated with azoospermia in patients with deletion of the AZFc region (AZFc_del). However, the molecular mechanisms of DAZ in spermatogenesis remain uncertain. In this study, the molecular mechanism of DAZ is identified, which is unknown since it is identified 40 years ago because of the lack of a suitable model. Using clinical samples and cell models, it is shown that DAZ plays an important role in spermatogenesis and that loss of DAZ is associated with defective proliferation of c‐KIT‐positive spermatogonia in patients with AZFc_del. Mechanistically, it is shown that knockdown of DAZ significantly downregulated global translation and subsequently decreased cell proliferation. Furthermore, DAZ interacted with PABPC1 via the DAZ repeat domain to regulate global translation. DAZ targeted mRNAs that are involved in cell proliferation and cell cycle phase transition. These findings indicate that DAZ is a master translational regulator and essential for the maintenance of spermatogonia. Loss of DAZ may result in defective proliferation of c‐KIT‐positive spermatogonia and spermatogenic failure.
Formulate Adaptive Biphasic Scaffold via Sequential Protein‐Instructed Peptide Co‐Assembly
Authors: Chen Yazhou , Zhang Qizheng , Yang Shenyu , Li Guanying , Shi Chaochen , Hu Xunwu , Asahina Shunsuke , Asano Natsuko , Zhang Ye 
Published: May 2024

AbstractTo ensure compositional consistency while mitigating potential immunogenicity for stem cell therapy, synthetic scaffolds have emerged as compelling alternatives to native extracellular matrix (ECM). Substantial progress has been made in emulating specific natural traits featuring consistent chemical compositions and physical structures. However, recapitulating the dynamic responsiveness of the native ECM involving chemical transitions and physical remodeling during differentiation, remains a challenging endeavor. Here, the creation of adaptive scaffolds is demonstrated through sequential protein‐instructed molecular assembly, utilizing stage‐specific proteins, and incorporating in situ assembly technique. The procedure is commenced by introducing a dual‐targeting peptide at the onset of stem cell differentiation. In response to highly expressed integrins and heparan sulfate proteoglycans (HSPGs) on human mesenchymal stem cell (hMSC), the peptides assembled in situ, creating customized extracellular scaffolds that adhered to hMSCs promoting osteoblast differentiation. As the expression of alkaline phosphatase (ALP) and collagen (COL‐1) increased in osteoblasts, an additional peptide is introduced that interacts with ALP, initiating peptide assembly and facilitating calcium phosphate (CaP) deposition. The growth and entanglement of peptide assemblies with collagen fibers efficiently incorporated CaP into the network resulting in an adaptive biphasic scaffold that enhanced healing of bone injuries.
Podocyte Pathogenic Bone Morphogenetic Protein‐2 Pathway and Immune Cell Behaviors in Primary Membranous Nephropathy
Authors: Cai Anxiang , Meng Yiwei , Zhou Hang , Cai Hong , Shao Xinghua , Wang Qin , Xu Yao , Zhou Yin , Zhou Wenyan , Chen Luonan , Mou Shan 
Published: May 2024

AbstractPrimary membranous nephropathy (PMN) is one of the leading causes of end‐stage renal disease, and the most frequent cause of massive proteinuria in nondiabetic adults, resulting in fatal complications. However, the underlying pathomechanisms of PMN remain largely unclear. Here, single‐cell RNA sequencing is employed to analyze kidney biopsies from eleven PMN patients and seven healthy subjects. Profiling 44 060 cells from patients allowed us to characterize the cellular composition and cell‐type‐specific gene expression in the PMN kidney. The complement‐induced BMP2/pSMAD1/COL4 pathway is identified as the pathogenic pathway in podocytes, bridging two key events, i.e., complement system activation and glomerular basement membrane thickening in PMN. Augmented infiltration and activation of myeloid leukocytes and B lymphocytes are found, profiling delicate crosstalk of immune cells in PMN kidneys. Overall, these results provide valuable insights into the roles of podocytes and immune cells in PMN, and comprehensive resources toward the complete understanding of PMN pathophysiology.
Adipose‐derived mesenchymal stem cell‐incorporated PLLA porous microspheres for cartilage regeneration
Authors: Gao Chang , Yuan Wenlong , Wang Dongcheng , Zhang Xin , Zhang Tong , Zhou Zhimin 
Published: May 2024

AbstractBackgroundIn facial plastic surgery, patients with nasal deformity are often treated by rib cartilage transplantation. In recent years, cartilage tissue engineering has developed as an alternative to complex surgery for patients with minor nasal defects via injection of nasal filler material. In this study, we prepared an injectable nasal filler material containing poly‐L‐lactic acid (PLLA) porous microspheres (PMs), hyaluronic acid (HA) and adipose‐derived mesenchymal stem cells (ADMSCs).MethodsWe seeded ADMSCs into as‐prepared PLLA PMs using our newly invented centrifugation perfusion technique. Then, HA was mixed with ADMSC‐incorporated PLLA PMs to form a hydrophilic and injectable cell delivery system (ADMSC‐incorporated PMH).ResultsWe evaluated the biocompatibility of PMH in vitro and in vivo. PMH has good injectability and provides a favorable environment for the proliferation and chondrogenic differentiation of ADMSCs. In vivo experiments, we observed that PMH has good biocompatibility and cartilage regeneration ability.ConclusionIn this study, a injectable cell delivery system was successfully constructed. We believe that PMH has potential application in cartilage tissue engineering, especially in nasal cartilage regeneration.
A Dual‐Bond Crosslinking Strategy Enabling Resilient and Recyclable Electrolyte Elastomers for Solid‐State Lithium Metal Batteries
Authors: Yin Lijiang , Zhang Panpan , Yang Jun , Meng Jia , Wu Mengjing , Pu Xiong 
Published: May 2024

AbstractElastomeric solid polymer electrolytes (SPEs) are highly promising to address the solid‐solid‐interface issues of solid‐state lithium metal batteries (LMBs), but compromises have to be made to balance the intrinsic trade‐offs among their conductive, resilient and recyclable properties. Here, we propose a dual‐bond crosslinking strategy for SPEs to realize simultaneously high ionic conductivity, elastic resilience and recyclability. An elastomeric SPE is therefore designed with hemiaminal dynamic covalent networks and Li+‐dissociation co‐polymer chains, where the −C−N‐ bond maintains the load‐bearing covalent network under stress but is chemically reversible through a non‐spontaneous reaction, the weaker intramolecular hydrogen bond is mechanically reversible, and the soft chains endow the rapid ion conduction. With this delicate structure, the optimized SPE elastomer achieves high elastic resilience without loading‐unloading hysteresis, outstanding ionic conductivity of 0.2 mS cm−1 (25 °C) and chemical recyclability. Then, exceptional room‐temperature performances are obtained for repeated Li plating/stripping tests, and stable cycling of LMBs with either LiFePO4 or 4.3 V‐class LiFe0.2Mn0.8PO4 cathode. Furthermore, the recycled and reprocessed SPEs can be circularly reused in LMBs without significant performance degradation. Our findings provide an inspiring design principle for SPEs to address the solid‐solid‐interface and sustainability challenges of solid‐state LMBs.
A Light‐Powered Single‐Stranded DNA Molecular Motor with Colour‐Selective Single‐Step Control
Authors: Anderson Tommy , Wu Wei , Sirbu Olga , Tong Keshao , Siti Winna , Rui Liu Xiao , Kou Bo , Murayama Keiji , Asanuma Hiroyuki , Wang Zhisong 
Published: May 2024

AbstractTop‐down control of small motion is possible through top‐down controlled molecular motors in replacement of larger actuators like MEMS or NEMS (micro‐ or nano‐electromechanical systems) in the current precision technology. Improving top‐down control of molecular motors to every single step is desirable for this purpose, and also for synchronization of motor actions for amplified effects. Here we report a designed single‐stranded DNA molecular motor powered by alternated ultraviolet and visible light for processive track‐walking, with the two light colours each locking the motor in a full directional step to allow saturated driving but no overstepping. This novel nano‐optomechanical driving mechanism pushes the top‐down control of molecular motors down to every single step, thus providing a key technical capability to advance the molecular motor‐based precision technology and also motor synchronization for amplified effects.
Management of Triplet States in Modified Mononuclear Ruthenium(II) Complexes for Enhanced Photocatalysis
Authors: Liang Ping , Wang Zhaolong , Hao Siwei , Chen Kai‐Kai , Wu Kaifeng , Wei Zhanhua 
Published: May 2024

AbstractControlling the interplay between relaxation and charge/energy transfer processes in the excited states of photocatalysts is crucial for the performance of artificial photosynthesis. Metal‐to‐ligand charge‐transfer triplet states (3MLCT*) of ruthenium(II) complexes are broadly implemented for photocatalysis, but an effective means of managing the triplets for enhanced photocatalysis has been lacking. Herein, We proposed a strategy to considerably prolong the triplet excited‐state lifetime by decorating a ruthenium(II) phosphine complex (RuP‐1) with pendent polyaromatic hydrocarbons (PAHs). Systematic studies demonstrate that in RuP‐4 decorated with anthracene, sub‐picosecond electron transfer from anthracene to 3MLCT* leads to a charge‐separated state that can mediate the formation of the intra‐ligand triplet state (3IL) of anthracene, resulting in an exceptionally long excited‐state up to several milliseconds. This triplet management strategy enables impressive photocatalytic reduction of CO2 to CO with a turnover number (TON) of 404, an optimized quantum yield of 43 % and 100 % selectivity, which is the highest reported performance for mononuclear photocatalysts without additional photosensitizers. RuP‐4 also catalyzes photochemical hydrogen generation under argon. This work opens up an avenue for regulating the excited‐state charge/energy flow for the development of long‐lived 3IL multi‐functional mononuclear photocatalysts to boost artificial photosynthesis.
Water vapor permeability of polymeric packaging materials for novel glass‐free photovoltaic applications
Authors: Babin Markus , Eder Gabriele C. , Voronko Yuliya , Oreski Gernot 
Published: May 2024

AbstractMoisture ingress in photovoltaic (PV) modules is a critical factor for performance degradation, therefore, a low water vapor transmission rate (WVTR) is highly desirable for polymers used to embed the solar cells, including backsheets, frontsheets, and encapsulants. With the advent of glass‐free modules for integration in building envelopes and vehicles, there is growing interest in polymer composite structures with embedded glass fibers to enhance rigidity. Furthermore, due to environmental concerns, there is increased interest in fluorine‐free polymers for PV applications. In this work, 21 samples with different base polymers, coatings, and/or surface treatments are investigated and their WVTRs are measured. The results show no good alternatives to existing fluoride‐based polymers/coatings for reducing WVTRs of backsheets and frontsheets among the investigated samples. In addition, glass fibers embedded within polymers to provide increased stability to backsheets or in composites for lightweight PV are shown to significantly increase WVTRs, especially, when fibers are not properly embedded, providing additional diffusion pathways for moisture ingress.
Utility of native emission quenching of erythrosine B for the determination of diltiazem in different dosage forms
Authors: Abdel‐Lateef Mohamed A. , Darling Ryhan J. , Darwish Ibrahim A. 
Published: May 2024

AbstractThis study introduces a practical and cost‐effective method for tracking diltiazem (DLZ) analytically. It utilizes a fluorimetric approach that relies on the modulation of fluorescence intensity of a dye called erythrosine B. Through a one‐pot experiment performed in an acidic environment, a complex is rapidly formed between DLZ and erythrosine B. By observing the decrease in erythrosine B emission, a linear calibration plot is established, enabling the detection and quantification of DLZ concentrations ranging from 40 to 850 ng/ml. The estimated limits of detection and quantitation were 10.5 and 32.1 ng/ml, respectively. The variables affecting the DLZ‐dye complex system were carefully adjusted. The validity of the approach was confirmed through a thorough evaluation based on the criteria set by ICH guidelines. The accuracy and precision of the methodology were evaluated, and the standard deviation and relative standard deviation were below 2. The strategy was successfully employed to analyze DLZ in tablets and capsules, and no significant variation between the proposed and reported methods as the values of the estimated t‐test and F‐test at five determinations were below 2.306 and 6.338, respectively. Notably, the method adheres to the principle of green chemistry by utilizing distilled water as the dispersing medium.
Quantitation of tazemetostat in human plasma using liquid chromatography–tandem mass spectrometry
Authors: Orleni Marco , Parise Robert A. , Holleran Julianne L. , Amengual Jennifer E. , Feng Ye , Synold Timothy , Beumer Jan H. 
Published: May 2024

AbstractTo support a phase 1 trial in patients with lymphomas, we developed a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for tazemetostat quantitation in 20 μL of human plasma. After protein precipitation, chromatographic separation employed a Kinetex C18 column and a gradient of 0.1% formic acid in both water and acetonitrile, during a 3‐min run time. Detection was achieved using a SCIEX 6500+ tandem mass spectrometer with electrospray positive‐mode ionization. Validation was based on the latest Food and Drug Administration guidance. With a stable isotopic internal standard, the assay was linear within the range of 10–5000 ng/mL and proved to be accurate (91.9%–103.7%) and precise (<4.4% imprecision). Recovery varied between 93.3% and 121.1%, and matrix effect ranged from −25.5% to −4.9%. Hemolysis, lipemia, and dilution did not impact quantitation. Plasma stability was confirmed after three freeze–thaw cycles, 24 h at room temperature, and 4 months at −80°C. Incurred sample reanalysis yielded 94.4% samples within 20% difference (n = 36). External validation showed a mean bias of −11.1%. Pharmacokinetic (PK) data obtained from three patients suggested variable concentration time profiles, warranting collection of further data. The assay proved to be suitable for tazemetostat quantitation in human plasma and will support clinical studies by defining tazemetostat PKs.
Association between the executive dysfunction and balance function in patients with stroke
Authors: Sakai Katsuya , Hosoi Yuichiro , Harada Yusuke , Kato Yuichi 
Published: May 2024

AbstractIntroductionA previous study has shown an association between executive dysfunction (ED) and balance function in patients with stroke. However, it is unclear what factors mediate the association between ED and balance function. Therefore, the aim of this study was to investigate the association between ED and balance function and to identify mediating factors using mediation analysis.MethodsThis study had a cross‐sectional design. The study included 107 patients with stroke. This study was divided into two groups (ED and non‐ED) using trail making test (TMT) part B. Two groups were compared for balance function (timed up and go test [TUGT] and Berg balance scale [BBS]) and other variables using paired test. In addition, partial correlation analysis with age, cognitive function as a control factor, and mediation analysis were also performed.ResultsThe ED group (N = 55) had significantly lower TUGT and BBS scores than the non‐ED group (N = 52). TMT part B correlated with TUGT (ρ = 0.41), BBS (ρ = −0.33), and Brunnstrom recovery stage (BRS) lower limb (ρ = −0.22). The TUGT model of mediation analysis showed a significant indirect effect as a result of mediation of the BRS lower limb between TMT part B and TUGT. The BBS model showed a significant indirect effect as a result of mediation of the activities of daily living (ADL) motor function between TMT part B and BBS.ConclusionsED and balance function were associated, and the degree of paralysis and ADL motor function were associated with them in patients with stroke.
Development of a MATLAB‐based graphical user interface to illustrate Coulomb's law and Gauss's law
Authors: Tan Xin Yi , Chan Kheong Sann , Lim Soo Yong 
Published: May 2024

AbstractCoulomb's law and Gauss's law are two fundamental laws in electrostatics that involve electric charges and electric fields. These concepts that are not visible physically in real life can be challenging for students to understand, especially when it involves complex charge distributions. This paper presents a MATLAB‐based graphical user interface (GUI) to visualise the concept of Coulomb's law and Gauss's law as well as a serious snake game to reinforce users' knowledge. This MATLAB GUI features a diverse range of 3‐dimensional (3D) visualisations illustrating electric field attributes such as electric force, charge distribution and electric flux. These visualisations dynamically adapt to user‐defined parameters, offering a rich and varied exploration of the electric field's characteristics without confining the scope to a specific count of models. In Coulomb's law section, the GUI plots the electric forces in 3‐D for dipole, three‐point charges, and unlimited charge. The Gauss's law section consists of windows for illustration of the fundamentals of Gauss's law for the electric field, and the illustration for the application of Gauss's law in finding the electric field for a point charge, infinite line charge and infinite plane charge. The serious snake game developed in this work allows students to engage in quizzes related to Coulomb's law and Gauss's law to serve as a tool for reinforcing their learning outcomes. The MATLAB‐based GUI was chosen as the platform due to its excellent visualisation capabilities, ease of programming and capability to act as a powerful tool to enhance the learning of Coulomb's law and Gauss's law for students.