bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kyphoplasty for spinal fractures from multiple myeloma
Authors: Zou Jun , Mei Xin , Gan Minfeng , Yang Huilin 
Published: April 2010

AbstractIntroductionVertebral destruction secondary to multiple myeloma is a significant clinical issue, and controversy still exists over how many levels should undergo kyphoplasty in multiple myeloma. In addition, in vertebrae that have lost wall integrity, cement extravasation remains an important consideration and is relatively contraindicated.MethodsForty‐three symptomatic vertebral fractures from multiple myeloma were diagnosed by magnetic resonance imaging (MRI). In levels with anterior vertebral wall compromise, two distinct sequential applications of cement were performed. In levels with demonstrated posterior or lateral wall deficiency, cement was injected under fluoroscopy.ResultsAll patients tolerated the procedure well and had immediate relief of back pain after kyphoplasty. Symptomatic cement extravasation and other complications were not observed. Vertebral height was restored (anterior 56.9 ± 14.2% to 82.9 ± 11.2%, middle 71.0 ± 13.4% to 81.1 ± 6.4%) (P < 0.001), and the mean kyphotic angle was improved (17.1 ± 7.2° to 8.9 ± 6.4°) (P < 0.001). The mean VAS decreased significantly from presurgery to postsurgery (8.1 ± 1.5 to 3.6 ± 1.8) (P < 0.001), as did the ODI (63.2 ± 15.9 to 37.1 ± 10.2) (P < 0.001). Six of eight subscores measured by the SF‐36, were significantly improved by the operation. All improvements were sustained up to final follow‐up.ConclusionKyphoplasty is a safe and clinically effective treatment for pathologic vertebral fractures from multiple myeloma, even in levels with vertebral wall deficiency. The strategy of determining systematic level by alterations in MRI signal is effective in lowering the cost. J. Surg. Oncol. J. Surg. Oncol. 2010;102:43–47. © 2010 Wiley‐Liss, Inc.
Detection of the reduced forms of radical adducts on the ESR trace using HPLC‐electrochemical detector‐ultraviolet absorption detector‐electron spin resonance‐MS
Authors: Ikeda Hideyuki , Iwahashi Hideo 
Published: April 2010

AbstractTo detect and identify the electron spin resonance (ESR) silent forms of the α‐(4‐pyridyl‐1‐oxide)‐N‐tert‐butylnitrone (4‐POBN) radical adducts, an electrochemical detector (ECD) was employed as a reactor in the HPLC‐ECD‐UV absorption detector‐ESR‐MS (HPLC‐ECD‐UV‐ESR‐MS). The ECD was employed to regenerate the radical forms from the reduced forms. The reduced forms of the 4‐POBN/pentyl radical adducts were analyzed using the HPLC‐ECD‐UV‐ESR‐MS. On addition of the ECD applied potential of +0.3 V, a peak appeared on the ESR trace of the HPLC‐ECD‐UV‐ESR‐MS analyses, indicating that the radical forms are regenerated from the reduced forms. The HPLC‐ECD‐UV‐ESR‐MS analyses were also performed for the reaction mixtures of phenylhydrazine with CuCl2. Two peaks (peaks I and II) were detected on the UV trace (300 nm) of the HPLC‐ECD‐UV‐ESR‐MS. The mass spectra showed that the peak I and peak II compounds are radical and reduced forms of the 4‐POBN/phenyl radical adducts under the ECD applied potential of 0.0 V. Peak I was only detected on the ESR trace under the ECD applied potential of 0.0 V. In addition to peak I, peak II appeared on the ESR trace under the ECD applied potential of +0.3 V, indicating that the reduced forms are oxidized to the corresponding radical forms.
Micellar liquid chromatography for lipophilicity determination of new biologically active 1,3‐purinodiones
Authors: Kawczak Piotr , Vander Heyden Yvan , Nasal Antoni , Bączek Tomasz , Drabczyñska Anna , Kieć‐Kononowicz Katarzyna , Kaliszan Roman 
Published: April 2010

AbstractA series of newly synthesized 1,3‐purinodiones with potential anticonvulsant activity, exhibiting affinity to adenosine A1 and/or A2A receptors, were subjected to micellar LC (MLC) with SDS as micelle‐forming agent and n‐propanol as organic modifier. Two C18 silica‐based columns were employed in MLC: a particle one and a monolithic. In parallel, those derivatives were also analyzed in RP‐LC on four silica‐based columns and on an immobilized artificial membrane column. The correlations between the relevant logarithms of the retention factors of analytes obtained in MLC, immobilized artificial membrane and RP‐LC systems on the one hand, and the calculated log P (clog P) and log D values (clog D) on the other, were examined. The level of the correlations of retention data from MLC and RP‐LC systems with clog P and clog D obtained is similar but it could be stressed that MLC allows increasing the speed of analysis and using only one mobile phase. Moreover, there is no need of applying an extrapolation procedure in lipophilicity determination. Therefore, the MLC systems, providing chromatographic data in a fast and efficient manner, were demonstrated as promising alternatives to the classical RP‐LC systems to estimate the lipophilicity of drugs and drug candidates.
Influence of heat flux on localised corrosion of stainless steel under boiling conditions
Authors: Stoulil J. , Bystriansky J. 
Published: April 2010

AbstractThe article deals with the influence of heat flux on localised forms of corrosion of stainless steels under the conditions of phase transition on a metal surface. The observations focused on the influence of the initiation and propagation of pitting corrosion and stress corrosion cracking. Pitting corrosion was tested in the environment of 0.6% FeCl3 + 0.3% EDTA + 0.1% HCl, the susceptibility to stress corrosion cracking was verified in 35% MgCl2. The tests proved a negative influence of heat flux on the initiation and propagation of non‐uniform corrosion.
Electrochemical corrosion behavior of electroless Ni–P coating in NaCl and H2SO4 solutions
Authors: Wang L. L. , Chen H. J. , Hao L. , Lin A. , Gan F. X. 
Published: April 2010

AbstractElectrochemical corrosion behavior of electroless Ni–P coating in NaCl and H2SO4 solutions were studied by potentiodynamic polarization curves and electrochemical impedance spectra techniques, as well as the corrosion morphology was characterized. The results indicate that electroless Ni–P coating with about 25 µm is stable in 30 days immersion in NaCl solution. Although it was corroded with prolonged immersion days, the corrosive medium has not penetrated through the coating. During the H2SO4 concentration ranging from 5 to 10%, the corrosion current density of electroless Ni–P coating increased due to the intensified anodic dissolution process; in 15% H2SO4 solution, electroless Ni–P coating shows obvious anodic passivation effect.
Effect of the molecular structure of some quinones on their corrosion inhibiting action
Authors: Stoyanova A. , Slavcheva E. 
Published: April 2010

AbstractThe molecular characteristics obtained by quantum chemical self‐consistent field (SCF) calculations of a series of quinones are correlated with the experimentally determined inhibitor efficiency for mild steel corrosion in neutral aqueous medium. It was established that the decrease of the ionization potential and the increase of the dipole moment of the quinone molecules favor the higher protective effect. The electron density and the geometric molecular structure have also been computed and are discussed in view of the corrosion inhibition.
Chromate replacement in coatings for corrosion protection of aerospace aluminium alloys
Authors: Markley T. A. , Mardel J. I. , Hughes A. E. , Hinton B. R. W. , Glenn A. M. , Forsyth M. 
Published: April 2010

AbstractMixed rare earth organophosphates have been investigated as potential corrosion inhibitors for AA2024‐T3 with the aim of replacing chromate‐based technologies. Cerium diphenyl phosphate (Ce(dpp)3) and mischmetal diphenyl phosphate (Mm(dpp)3) were added to epoxy coatings applied to AA2024‐T3 panels and they were effective in reducing the amount and rate of filiform corrosion in high humidity conditions. Ce(dpp)3 was the most effective and characterisation of the coating formulations showed approximately a factor of 5 reduction in both the number of corrosion filaments initiated as well as the length of these. Mm(dpp)3 appeared to reduce the corrosion growth rate by a factor of 2 although it was the more effective inhibitor in solution studies. Spectroscopic characterisation of the coatings indicated that the cerium based inhibitor may disrupt network formation in the epoxy thus resulting in a coating that absorbed more water and allowed greater solubilisation of the corrosion inhibiting compound.
Mono‐carboxylate conversion coatings for AZ31 Mg alloy protection
Authors: Frignani A. , Grassi V. , Zucchi F. , Zanotto F. 
Published: April 2010

AbstractConversion coatings on a magnesium alloy were obtained by dipping AZ31 specimens in aqueous solutions of sodium salts of mono‐carboxylic acids (stearic, palmitic, myristic, lauric, mono‐carboxylate ion concentration from 1 to 5 mM, depending on the salt solubility) for 24 and 72 h at room temperature, or 24 h at 50 °C. The influence exerted by the treatment time, bath temperature and alkyl chain length on the efficiency of these coatings was studied.The performances of the coatings were evaluated by potentiodynamic polarization curve recording after 1 h immersion in 0.05 M Na2SO4 solution, while their temporal evolution was monitored by electrochemical impedance spectroscopy (EIS) spectra during 24 h. Further and long lasting tests were carried out also in 0.1 M NaCl solution.The efficiency of the coatings depended on the aliphatic chain length, and increased as the treatment time and the bath temperature were increased. The coating of lower homologue only hindered the cathodic process, while those of the higher homologues markedly inhibited the anodic process too. The best performances were displayed by 24 h‐50°C stearic conversion coating, which maintained a very high efficiency for over 800 h immersion in 0.05 M sulphate solution.
Perry syndrome due to the DCTN1 G71R mutation: A distinctive levodopa responsive disorder with behavioral syndrome, vertical gaze palsy, and respiratory failure
Authors: Newsway Victoria , Fish Mark , Rohrer Jonathan D. , Majounie Elisa , Williams Nigel , Hack Melissa , Warren Jason D. , Morris Huw R. 
Published: April 2010

AbstractPerry syndrome is a rare form of autosomal dominant Parkinsonism with respiratory failure recently defined as being due to mutations in the DCTN1 gene. We describe a new family carrying a G71R mutation in the DCTN1 gene. The proband displayed a series of distinctive features not previously described in Perry syndrome: a disorder of vertical downward saccades accompanied by progressive midbrain atrophy, predominant nonmotor symptoms responsive to levodopa, distinctive craniocervical levodopa induced dyskinesias, and a good response to high‐dose levodopa therapy and respiratory support. The family was initially thought to have autosomal dominant behavioral variant frontotemporal dementia with Parkinsonism. This report expands the clinical definition of this distinctive syndrome. © 2009 Movement Disorder Society
Isolation, cloning, and characterization of the 2S albumin: A new allergen from hazelnut
Authors: Garino Cristiano , Zuidmeer Laurian , Marsh Justin , Lovegrove Alison , Morati Maria , Versteeg Serge , Schilte Piet , Shewry Peter , Arlorio Marco , van Ree Ronald 
Published: April 2010

AbstractScope: 2S albumins are the major allergens involved in severe food allergy to nuts, seeds, and legumes. We aimed to isolate, clone, and express 2S albumin from hazelnut and determine its allergenicity. Methods: 2S albumin from hazelnut extract was purified using size exclusion chromatography and RP‐HPLC. After N‐terminal sequencing, degenerated and poly‐d(T) primers were used to clone the 2S albumin sequence from hazelnut cDNA. After expression in Escherichia coli and affinity purification, IgE reactivity was evaluated by Immunoblot/ImmunoCAP (inhibition) analyses using sera of nut‐allergic patients. Results: N‐terminal sequencing of a ∼10 kDa peak from size exclusion chromatography/RP‐HPLC gave two sequences highly homologous to pecan 2S albumin, an 11 amino acid (aa) N‐terminal and a 10aa internal peptide. The obtained clone (441 bp) encoded a 147aa hazelnut 2S albumin consisting of a putative signal peptide (22 aa), a linker peptide (20 aa), and the mature protein sequence (105 aa). The latter was successfully expressed in E. coli. Both recombinant and natural 2S albumin demonstrated similar IgE reactivity in Immunoblot/ImmunoCAP (inhibition) analyses. Conclusion: We confirmed the postulated role of hazelnut 2S albumin as an allergen. The availability of recombinant molecules will allow establishing the importance of hazelnut 2S albumin for hazelnut allergy.
β‐Carboline alkaloids from the stems of Picrasma quassioides
Authors: Jiao Wei‐Hua , Gao Hao , Li Chen‐Yang , Zhou Guang‐Xiong , Kitanaka Susumu , Ohmura Atsuko , Yao Xin‐Sheng 
Published: April 2010

AbstractFive new β‐carboline alkaloids, 6,12‐dimethoxy‐3‐(2‐hydroxylethyl)‐β‐carboline (1), 3,10‐dihydroxy‐β‐carboline (2), 6,12‐dimethoxy‐3‐(1‐hydroxylethyl)‐β‐carboline (3), 6,12‐dimethoxy‐3‐(1,2‐dihydroxylethyl)‐β‐carboline (4), and 6‐methoxy‐3‐(2‐hydroxyl‐1‐ethoxylethyl)‐β‐carboline (5), and two new natural products, 6‐methoxy‐12‐hydroxy‐3‐methoxycarbonyl‐β‐carboline (6) and 3‐hydroxy‐β‐carboline (7) were isolated from the stems of Picrasma quassioides along with 16 known β‐carboline alkaloids (8–23). The structures of new compounds were determined by extensive spectroscopic analyses, and the 1D and 2D NMR data of compounds 6, 7 and 10 were reported for the first time. The bioassays showed that only compounds 14 and 16 could enhance the differentiation of 3T3‐L1 preadiocytes accompanied by secretion of adiponectin proteins among these 23 compounds. Copyright © 2010 John Wiley & Sons, Ltd.
Randomizing research participants: Promoting balance and concealment in small samples
Authors: Matthews Ellyn E. , Cook Paul F. , Terada Masaka , Aloia Mark S. 
Published: April 2010

AbstractRandomization is central to rigorous scientific trials. An effective but underutilized approach is urn randomization. To test the ability of urn randomization versus simple randomization to produce balanced groups with small sample sizes, we conducted simulated randomizations: 10 times under sample size scenarios of 20, 40, 60 (group sizes of 10, 20, and 30, respectively), for 30 trials in total. For groups of 20–30, urn surpassed simple randomization in the equal distribution of confounding variables between groups, leading to effects of these variables that were both smaller on average and more consistently close to zero over multiple trials. The urn method is easy to implement and has the advantages of unpredictability of assignment and decreased susceptibility to investigator bias. © 2010 Wiley Periodicals, Inc. Res Nurs Health 33:243–253, 2010
Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype
Authors: Anami Katsuhiro , Oue Naohide , Noguchi Tsuyoshi , Sakamoto Naoya , Sentani Kazuhiro , Hayashi Tetsutaro , Hinoi Takao , Okajima Masazumi , Graff Jonathan M , Yasui Wataru 
Published: April 2010

AbstractGastric cancer (GC) is one of the most common malignancies worldwide. Genes expressed only in cancer tissue, and especially on the cell membrane, will be useful molecular markers for diagnosis and may also be good therapeutic targets. To identify genes that encode transmembrane proteins present in GC, we generated Escherichia coli ampicillin secretion trap (CAST) libraries from two GC cell lines and normal stomach. By sequencing 4320 colonies from CAST libraries, we identified 30 candidate genes that encode transmembrane proteins present in GC. Quantitative reverse transcription‐polymerase chain reaction analysis of these candidates revealed that ZDHHC14, BST2, DRAM2, and DSC2 were expressed much more highly in GC than in 14 kinds of normal tissues. Among these, DSC2 encodes desmocollin 2, which is one of three known desmocollins. Immunohistochemical analysis demonstrated that 22 (28%) of 80 GC cases were positive for desmocollin 2, and desmocollin 2 expression was observed frequently in GC with the intestinal mucin phenotype. Furthermore, desmocollin 2 expression was correlated with CDX2 expression. These results suggest that expression of desmocollin 2, induced by CDX2, may be a key regulator for GC with the intestinal mucin phenotype. Our results provide a list of genes that have high potential as a diagnostic and therapeutic target for GC. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Polymyxin‐direct hemoperfusion for sepsis‐induced multiple organ failure
Authors: Hirabayashi Koichi , Shiohara Masaaki , Saito Shoji , Tanaka Miyuki , Yanagisawa Ryu , Tsuruta Goro , Fukuyama Tetsuhiro , Hidaka Yoshihiko , Nakazawa Yozo , Shimizu Takashi , Sakashita Kazuo , Koike Kenichi 
Published: April 2010

AbstractWe report a case of multiple organ failure caused by the Bacillus cereus infection during acute lymphoblastic leukemia therapy, who was treated successfully. A 15‐year‐old male developed (B. cereus) sepsis on the 19th day after chemotherapy initiation. Polymyxin‐direct hemoperfusion for septic shock was started, followed by continuous hemodiafiltration. His condition improved after starting the hemoperfusion. At the onset of sepsis, elevated levels of serum inflammatory cytokines, anti‐inflammatory cytokines, and plasminogen‐activator inhibitor complex‐1 were observed. Serum levels of these cytokines and bioactive substances decreased after blood purification therapy, which correlated with the improvement of clinical symptoms. Pediatr Blood Cancer 2010;55:202–205. © 2010 Wiley‐Liss, Inc.
Imatinib mesylate in children and adolescents with cancer
Authors: Barr Ronald D. 
Published: April 2010

AbstractImatinib is an inhibitor of theBCR–ABLfusion gene product that characterizes chronic myeloid leukemia (CML), and of the related tyrosine kinases c‐KIT and platelet‐derived growth factor (PDGF) receptor. The drug is now included as front‐line therapy for CML and Philadelphia chromosome‐positive acute lymphoblastic leukemia in children and adolescents, though valid concerns about serious late sequelae remain unresolved and are important issues for further study. European and North American consortia have conducted phase I and II clinical trials of imatinib in children and adolescents with brain and other solid tumors that have provided little evidence of efficacy. Pediatr Blood Cancer 2010;55:18–25. © 2010 Wiley‐Liss, Inc.
Detection of cell‐free RNA in children with neuroblastoma and comparison with that of whole blood cell RNA
Authors: Corrias Maria Valeria , Pistorio Angela , Cangemi Giuliana , Tripodi Gino , Carlini Barbara , Scaruffi Paola , Fardin Paolo , Garaventa Alberto , Pistoia Vito , Haupt Riccardo 
Published: April 2010

AbstractBackgroundSince there is no validated assay to monitor disease in children with neuroblastoma (NB), we tested whether NB specific cell‐free RNA could be detected in their plasma samples. Moreover, with the aim of reducing patients' discomfort, we compared this assay to a recently standardized procedure that uses a larger amount of whole blood.ProceduresUsing conditions that excluded RNA recovery from contaminating tumor cells, the total amount of cell‐free RNA present in healthy children and patients with NB was quantified. Expression of tyrosine hydroxylase (TH) was assayed by quantitative RT‐PCR.ResultsIn patients with NB the amount of cell‐free RNA was higher than in healthy children. However, it was less and more degraded than in healthy adults. The median amount of cell‐free RNA that was reverse transcribed, measured through the use of standard curves for reference genes, was 0.03 (range 0–30) pg of input RNA, that is, always less than 1/10,000 of that reverse transcribed from total RNA extracted from whole cells. Despite the presence of disease and the positive results obtained with RNA extracted from peripheral blood cells, few cell‐free RNA samples tested positive by the TH assay. Similar results were obtained also with TH primers specifically designed to amplify 50 bp RNA fragments.ConclusionThese findings suggest that for monitoring disease status detection of cell‐free tumor‐specific RNAs in patients with NB is not a reliable alternative to whole cell RNA. Pediatr Blood Cancer 2010;54:897–903 © 2010 Wiley‐Liss, Inc.
Intensive multimodality therapy for patients with stage 4a metastatic retinoblastoma
Authors: Dunkel Ira J. , Khakoo Yasmin , Kernan Nancy A. , Gershon Timothy , Gilheeney Stephen , Lyden David C. , Wolden Suzanne L. , Orjuela Manuela , Gardner Sharon L. , Abramson David H. 
Published: April 2010

AbstractBackgroundWe previously reported promising pilot results treating patients with stage 4a metastatic retinoblastoma with combined intensive conventional chemotherapy, high‐dose chemotherapy with autologous hematopoietic stem cell rescue, and radiation therapy and now present an expanded and updated series.ProcedureFifteen patients with bone marrow (n = 14), bone (n = 10), orbit (n = 9), and/or liver (n = 4) disease were treated. Induction chemotherapy usually consisted of vincristine, cyclophosphamide, cisplatin, and etoposide. The high‐dose chemotherapy regimen included carboplatin and thiotepa alone (n = 1) or with etoposide (n = 5) or topotecan (n = 7).ResultsBone marrow cleared at first post‐initiation of chemotherapy examination in all patients and stem cells were harvested after a median of 3.5 cycles of chemotherapy (range 3–6 cycles). Two patients progressed prior to high‐dose chemotherapy and died. Thirteen received high‐dose chemotherapy at a median of 6 months post‐diagnosis of metastases (range 4–8 months). Ten are retinoblastoma‐free in first remission at a median follow‐up of 103 months (range 34–202 months) while three recurred (two in the CNS, one in the mandible) 14–20 months post‐diagnosis of metastases. Retinoblastoma‐free and event‐free survival at 5 years are 67% (95% confidence interval 38–85%) and 59% (95% confidence interval 31–79%). Six of the 10 survivors received radiation therapy. Three patients developed secondary osteosarcoma 14, 4, and 9 years after diagnosis of metastatic disease.ConclusionsIntensive multimodality therapy including high‐dose chemotherapy with autologous hematopoietic stem cell rescue was curative for the majority of patients with stage 4a metastatic retinoblastoma treated. The contribution of external beam radiation therapy is unclear. Pediatr Blood Cancer 2010;55:55–59. © 2010 Wiley‐Liss, Inc.
Using photoelectron spectroscopy for the investigation of substituent effects in N‐ and P‐centered radical cations
Authors: Egorochkin Alexey N. , Kuznetsova Olga V. , Khamaletdinova Nadiya M. , Domratcheva‐Lvova Lada G. , Domrachev Georgy A. 
Published: April 2010

AbstractThe information concerning the peculiarities of the intramolecular interactions in the radical cations which is currently available is very sketchy. In this work, a new approach to the investigation of the substituent effects in N‐ and P‐centered radical cations has been developed. It is based on a consideration of the core‐electron binding energies E and ionization potentials I of the 15 series of the neutral molecules measured by photoelectron spectroscopy. Properties E and I obey the linear free energy relationship. By using the correlation analysis, in radical cations the inductive, resonance, and polarizability effects were first established to be in operation. The polarizability effect is caused by the charge on the radical cation centers N•+ and P•+. The contribution of this effect ranges from 10 to 55%. In the radical cations containing the moieties with N•+−X and P•+−X bonds, the standard resonance constants σR and σ of the substituents X are of limited utility. An understanding of the substituent effects may give a better insight into the mechanisms of both: radical ions and heterolytic reactions. Copyright © 2010 John Wiley & Sons, Ltd.