bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide association study in Chickpea (Cicer arietinum L.) for yield and nutritional components
Authors: Sari Hatice , Uhdre Renan , Wallace Lyle , Coyne Clarice J. , Bourland Britton , Zhang Zhiwu , Russo M. , Kiszonas Alecia , Warburton Marilyn L. 
Published: May 2024

AbstractThe increase in the global human population and the accompanying challenges in meeting nutritional needs amidst climate change are a worldwide concern. Widespread protein and micronutrient deficiencies contribute to a significant number of individuals experiencing malnutrition, leading to severe health repercussions. This issue can be addressed through genomics-assisted breeding, particularly in enhancing the nutritional profile of vital staple crops like chickpea (Cicer arietinum L.). Chickpea, beyond being a rich source of protein, provides a diverse nutritional spectrum encompassing carbohydrates, fats, and minerals. To explore and improve the genetic basis of nutritional traits in chickpea, a study was conducted using 93 kabuli-type single plant derived lines and five cultivars in 2018, 2022, and 2023. Genotyping by sequencing revealed a total of 165K single nucleotide polymorphisms (SNPs) within this kabuli chickpea mini-core collection. After filtering for a minor allele frequency greater than 5%, 113,512 SNPs were utilized, distributed across eight chromosomes of the chickpea genome. Marker-trait associations were analyzed using genome wide association study, leading to the identification of 27 significantly associated SNPs from across all eight chromosomes linked to three seed nutritional concentrations and 100-seed weight. To unravel the molecular mechanisms governing seed protein, fiber, fat concentrations, and 100-seed weight, 31 candidate genes were determined within a 30 kb window size. This comprehensive approach holds promise for advancing crop breeding strategies to combat malnutrition and improve global food security.
Phenology and the response of photosynthesis to irradiance and temperature gradient in the herbal drug red alga, Chondria armata (Rhodomelaceae, Ceramiales) from Kagoshima, Japan
Authors: Terada Ryuta , Yoshizato Kyosuke , Murakami Kazuma , Nishihara Gregory N. 
Published: May 2024

AbstractSeasonal changes in the size of the herbal drug red alga Chondria armata (Rhodomelaceae, Ceramiales) were investigated in Kagoshima, Japan, which is near the northern distributional limit in the western Pacific. Additionally, its photosynthetic response to irradiance and temperature was examined using dissolved oxygen sensors and a pulse amplitude modulation (PAM)-chlorophyll fluorometer. This alga was observed in tidepools throughout the year; its height and weight were greatest in December and the lowest in April and May. The net photosynthesis of the photosynthesis–irradiance (P–E) curve determined at 28°C quickly saturated at 113 µmol photons m-2 s-1, with minimal inhibition even at 1000 µmol photons m-2 s-1. The gross photosynthesis of the photosynthesis–temperature (P–T) curved over 8 to 40°C, measured at 500 µmol photons m-2 s-1, peaked at 30.1°C and decreased rapidly below 20°C and above 36°C, respectively. Similarly, the effective quantum yield (ΔF/Fm') after a 3-day culture during 4–40°C at 50 µmol photons m-2 s-1 remained stable between 16°C and 32°C but decreased outside of this range. The combined effect of irradiance (200 [low] and 1000 [high] µmol photons m-2 s-1) and temperature (28, 22, and 16°C) revealed that ΔF/Fm' declined during exposure to high irradiance at all temperature treatments. However, it mostly recovered after a subsequent 12-hour period of dim-light acclimation at 28°C and 22°C. In contrast, those at 16°C could not recover, indicating the occurrence of low-temperature light stress. This alga appears to be well-adapted to the irradiance and temperature environment at the study site. However, the winter temperature appears to approach its threshold level, and the occurrence of strong light during the winter might adversely affect the abundance of this alga near its northern distributional limit.
Non-invasive monitoring of microalgae cultivations using hyperspectral imager
Authors: Pääkkönen Salli , Pölönen Ilkka , Raita-Hakola Anna-Maria , Carneiro Mariana , Cardoso Helena , Mauricio Dinis , Rodrigues Alexandre Miguel Cavaco , Salmi Pauliina 
Published: May 2024

AbstractHigh expectations are placed on microalgae as a sustainable source of valuable biomolecules. Robust methods to control microalgae cultivation processes are needed to enhance their efficiency and, thereafter, increase the profitability of microalgae-based products. To meet this need, a non-invasive monitoring method based on a hyperspectral imager was developed for laboratory scale and afterwards tested on industrial scale cultivations. In the laboratory experiments, reference data for microalgal biomass concentration was gathered to construct 1) a vegetation index-based linear regression model and 2) a one-dimensional convolutional neural network model to resolve microalgae biomass concentration from the spectral images. The two modelling approaches were compared. The mean absolute percentage error (MAPE) for the index-based model was 15–24%, with the standard deviation (SD) of 13-18 for the different species. MAPE for the convolutional neural network was 11–26% (SD = 10–22). Both models predicted the biomass well. The convolutional neural network could also classify the monocultures of green algae by species (accuracy of 97–99%). The index-based model was fast to construct and easy to interpret. The index-based monitoring was also tested in an industrial setup demonstrating a promising ability to retrieve microalgae-biomass-based signals in different cultivation systems.
Tritrophic Interactions Mediated by Zoophytophagous Predator-Induced Host Plant Volatiles
Authors: Adams Bashiru , Yusuf Abdullahi Ahmed , Torto Baldwyn , Khamis Fathiya Mbarak 
Published: May 2024

AbstractThe zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including the invasive leafminer, Phthorimaea absoluta, a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. tenuis can influence the tritrophic interactions in the tomato plant. Here, we tested the hypothesis that N. tenuis phytophagy would influence the tritrophic olfactory interactions between the host plant tomato and pest, predator, and parasitoid. In olfactometer assays, P. absoluta females and N. tenuis adults were both attracted to constitutive volatiles released by the tomato plant. Whereas females of P. absoluta avoided volatiles released by N. tenuis-infested plants, S. japonicus females and N. tenuis adults were attracted to the induced volatiles. In coupled gas chromatography-electroantennographic detection (GC-EAD) recordings of intact and N. tenuis-infested plant volatiles, antennae of P. absoluta and S. japonicus females both detected eight components, whereas N. tenuis adults detected seven components which were identified by GC-mass spectrometry (GC-MS) as terpenes and green leaf volatiles (GLVs). Dose-response olfactometer bioassays revealed that the responses of P. absoluta, N. tenuis, and S. japonicus varied with the composition and concentration of blends and individual compounds tested from N tenuis-induced volatiles. Females of P. absoluta showed no preference for an eight-component blend formulated from the individual repellents including hexanal, (Z)-3-hexenyl butanoate, and δ-elemene identified in the volatiles. On the other hand, S. japonicus females were attracted to an eight-component blend including the attractants (E)-2-hexenal, (Z)-3-hexenol, methyl salicylate, β-phellandrene, and (E)-caryophyllene. Likewise, N. tenuis adults were attracted to a seven-component blend including the attractants β-phellandrene, δ-elemene, and (E)-caryophyllene identified in the volatiles. Our findings suggest that there is potential for the use of terpenes and GLVs to manage the insects in the tritrophic interaction.
Cue-Reactive Phenomenology Mediates the Relationship Between Positive Schizotypy and Cue-Reactive Urge to Gamble in Poker-Machine Gamblers
Authors: McTigue Benjamin A. , Talk Andrew C. , Rice Kylie , Rock Adam J. 
Published: May 2024

AbstractAlthough ubiquitous in numerous nightlife cultures, poker-machines present a high risk for problematic use and addiction. Previous research has demonstrated that gambling cues (e.g., flashing lights) can activate gambling urges in poker-machine gamblers. However, the processes that contribute to the maintenance of cue-reactive urges to gamble remain unclear. Consequently, the present study explored whether positive schizotypy predicted gambling urge, and whether cue-reactive altered state of awareness, cue-reactive altered time sense, and cue-reactive absorption mediated this relationship. Seventy adults aged between 19 and 68 (M = 48.86, SD = 12.82) participated in an online cue-reactivity experiment. Participants first completed the Problem Gambling Severity Index and the Unusual Experiences subscale of the Short Oxford-Liverpool Inventory of Feelings and Experiences. Subsequently, at three time points (i.e., baseline, directly after a neutral cue, and directly after a gambling cue) participants completed the Altered State of Awareness, Altered Time Sense, and Absorption subscales of the Phenomenology of Consciousness Inventory and a visual analogue scale measuring cue-reactive urge to gamble. It was found that positive schizotypy was significantly positively correlated with cue-reactive urge to gamble. Additionally, cue-reactive altered state of awareness, cue-reactive altered time sense, and cue-reactive absorption mediated this relationship. The theoretical, clinical and practical implications are discussed.
MMC Array to Study X-Ray Transitions in Muonic Atoms
Authors: Unger Daniel , Abeln Andreas , Cocolios Thomas Elias , Eizenberg Ofir , Enss Christian , Fleischmann Andreas , Gastaldo Loredana , Godinho César , Heines Michael , Hengstler Daniel , Indelicato Paul , Jadhav Ashish , Kreuzberger Daniel , Kirch Klaus , Knecht Andreas , Machado Jorge , Ohayon Ben , Paul Nancy , Pohl Randolf , von Schoeler Katharina , Vogiatzi Stergiani Marina , Wauters Frederik 
Published: May 2024

AbstractThe QUARTET collaboration aims to significantly improve the precision of the absolute nuclear charge radii of light nuclei from Li to Ne by using an array of metallic magnetic calorimeters to perform high-precision X-ray spectroscopy of low-lying states in muonic atoms. A proof-of-principle measurement with lithium, beryllium and boron is planned for fall 2023 at the Paul Scherrer Institute. We discuss the performance achieved with the maXs-30 detector module to be used. To place the detector close to the target chamber where the muon beam will impact the material under study, we have developed a new dilution refrigerator sidearm. We further discuss the expected efficiency given the transparency of the X-ray windows and the quantum efficiency of the detector. The expected muonic X-ray rate combined with the high resolving power and detection efficiency of the detector suggest that QUARTET will be able to study the de-excitation of light muonic atoms at an unprecedented level, increasing the relative energy resolution by up to a factor of 20 compared to conventional detector techniques.
In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth
Authors: Hardwick Laura J. A. , Davies Benjamin P. , Pensa Sara , Burge-Rogers Maedee , Davies Claire , Baptista André Figueiredo , Knott Robert , S. McCrone Ian , Po Eleonora , Strugnell Benjamin W. , Waine Katie , Wood Paul , Khaled Walid T. , Summers Huw D. , Rees Paul , Wills John W. , Hughes Katherine 
Published: May 2024

AbstractSignal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.
The 2015 Refugee Crisis and Institutional Trust in European Countries
Authors: Economou Athina , Kollias Christos 
Published: May 2024

AbstractExogenous events have the potential to affect citizens’ risk-perceptions, (in)security sentiments, electoral choices, policy preferences and institutional trust levels. It has been shown that migrant and refugee flows also affect citizens’ trust and policy preferences. The 2015 refugee crisis as it has come to be known, shook Europe’s cohesion and tested the existing immigration and asylum policies as well as acted as a further stimulus to populist/right wing Eurosceptic parties with an agenda of anti-immigrant restrictive policies. Expectedly, it has attracted considerable attention in the relevant literature. Building on existing studies that examine various aspects associated with the 2015 refugee crisis, the present paper examines its effect on institutional trust levels in Europe. Specifically, it focuses on trust towards the national government, the EU and the two state security services the police and the armed forces. To this effect, the study draws data from successive Eurobarometer surveys before and after the crisis. Employing segmented-type regression models as well as Oaxaca decomposition models it probes into the 2015 refugee crisis’ impact on the levels of institutional trust using over 400,000 responses to the relevant Eurobarometer questions. The results reported herein suggest the presence of “a rally around the flag effect”. Nonetheless, macroeconomic factors such as GDP growth and unemployment as well as differences in behavioural responses also explain a large part of the observed gap in trust before and after the 2015 refugee crisis.
Sustainable astronomy: A comparative life cycle assessment of off-grid hybrid energy systems to supply large telescopes
Authors: Viole Isabelle , Shen Li , Camargo Luis Ramirez , Zeyringer Marianne , Sartori Sabrina 
Published: May 2024

Abstract
Purpose
Supplying off-grid facilities such as astronomical observatories with renewable energy–based systems (RES) instead of diesel generators can considerably reduce their environmental impact. However, RES require oversized capacities to counter intermittency and comply with reliability requirements, hence shifting the environmental impact from operation to construction phase. We assess whether 100% RES scenarios are favorable from an environmental point of view and discuss the trade-offs in systems with backup fossil generators versus 100% renewable ones.

Methods
In this comparative life cycle assessment (LCA), we study various RES supply systems to power a new telescope in the Atacama Desert, Chile. We compare six setups, including 100% RES scenarios, namely, photovoltaics (PV) with batteries and hydrogen energy storage; high-renewable scenarios, with fossil fuel power generation next to RES and storage; and a system combining PV with diesel generation. We base system sizing on a techno-economical optimization for the start of operation in 2030. Foreground data stem from life cycle inventories of RES components since 2015 and 2030 electricity mix assumptions of production countries. We assess environmental impact in the categories climate change, mineral resource depletion, and water use.

Results and discussion
We find that 100% RES and high-renewable scenarios result in emissions of 0.077–0.115 kg CO2e/kWh supplied, compared to 0.917 kg CO2e/kWh in the reference case with solely diesel generation. One hundred percent RES scenarios have a lower CO2e impact than high-renewable scenarios. However, the latter lower the mineral resource depletion and water use by about 27% compared to 100% RES scenarios. Applying hybrid energy storage systems increases the water use impact while reducing the mineral resource depletion.

Conclusions
None of the six energy systems we compared was clearly the best in all environmental impacts considered. Trade-offs must be taken when choosing an energy system to supply the prospective off-grid telescope in Chile. We find high-renewable systems with some fossil generation as the better option regarding power reliability, mineral resource depletion, and water use, while inducing slightly higher greenhouse gas emissions than the 100% RES scenarios. As remote research facilities and off-grid settlements today are mainly supplied by fossil fuels, we expect to motivate more multifaceted decisions for implementing larger shares of RES for these areas. To advance the LCA community in the field of energy systems, we should strive to incorporate temporal and regional realities into our life cycle inventories. To ease the path for upcoming studies, we publish this work’s inventories as detailed activity level datasets.
How Social Connectedness Helps Patients Stay Home After Hospital at Home Enrollment: A Mixed Methods Study
Authors: Ledford Christy J. W. , Cafferty Lauren A. , Lee Eunice , Hayes Hailie C. , Ede Destine C. , Hodges Brandon P. , Whitebloom Grant C. , Walsh David W. , Wilkins Thad 
Published: May 2024

Abstract
Background
While enrolled in Hospital at Home (HaH) programs, patients rely on their social network to provide supportive behaviors that are routinely provided by hospital staff in the inpatient setting.

Objective
This study investigated how social connectedness is associated with patient outcomes in a HaH program.

Design
The explanatory iterative sequential mixed methods design included an electronic health record review to collect quantitative measures to describe the severity of patient illness and healthcare utilization and then qualitative interviews to explain quantitative findings.

Participants
The quantitative phase included 100 patients (18 years or older) admitted to the hospital who were subsequently enrolled in the HaH program. In the qualitative phase, 33 of the 100 patients participated in semi-structured interviews.

Analysis
Qualitative data was analyzed using the Sort & Sift, Think & Shift method. Integrated analysis included merged data displays of healthcare utilization data and patient descriptions of their care and genogram-type illustrations to enable variable-oriented analysis of structural support. We then examined patient narratives by two variables: life course and care elevation, to understand differences in the trajectories of six subsets of patients as identified by the quantitative data.

Key Results
Three factors prompted patients to enroll in HaH: low attention from hospital staff during hospital stay; loneliness and isolation during hospital stay; and family encouragement to enroll. After discharge, social support within the home structure facilitated recovery during HaH. Conversely, HaH patients with limited support within the home were more likely to be readmitted.

Conclusions
Structural social connectedness facilitates patient recovery in HaH. Before enrolling patients in HaH, clinicians should take an in-depth social history, including questions about social/familial roles, household responsibilities, and technology acceptance. Clinicians should engage formal and informal caregivers in these conversations early and communicate a clear picture of what caregivers should do to support the patient through recovery.
Factors Influencing Referral for Bariatric Surgery by Primary Care Physicians in Northern Israel
Authors: Zoabi Elham , Elran-Barak Roni , Sakran Nasser , Sandler Noga Kaftori , Hatoum Ossama Abu , Kaplan Uri 
Published: May 2024

Abstract
Purpose
Obesity is a chronic metabolic disease with global distribution among adults and children which affects daily functioning and ultimately quality of life. Primary care physicians (PCPs) provide an important role for the treatment of severe obesity. Better understanding of obesity and its treatment options may increase patients’ referral rates to the various treatment modalities, including metabolic/bariatric surgery (MBS).

Materials and Methods
A quantitative cross-sectional study used a self-reported questionnaire among PCPs of Clalit Health Services (CHS) in Northern Israel. The quantitative questionnaire examined the PCP’s knowledge, opinions, attitude, and approaches to managing severe obesity.

Results
A total of 246 PCPs from Northern Israel filled the questionnaire (42.9%), the majority were Muslim Arabs (54.5%), who gained their medical degree outside of Israel (73.8%) and practicing for over 10 years (58.8%). 64.3% of PCPs had a high workload (over 100 appointments per week), 77.1% did not know the definition of severe obesity, and 69.17% did not attend educational meetings regarding obesity during the previous year. The referral rate for MBS was 50.4% ± 23.3. Two prognostic factors that had a statistically significant effect on the referral rate for bariatric surgery were the total appointments per week, and the number of practice years. Both had a negative association.

Conclusion
The knowledge and referral rates for bariatric surgery are higher among PCPs with lower workload and relatively fewer practice years. Workshops and annual training courses may fortify knowledge and awareness for the treatment of obesity, which in turn could increase the referral rate for MBS.
Die Rolle von mikro-RNAs während der B-Zell-Entwicklung
Authors: Wittmann Jürgen 
Published: May 2024

AbstractB cell development in vertebrates is a complex process, involving numerous differentiation stages at many anatomical locations. Long-known regulators of B cell gene expression include transcription factor networks, cytokines, and cell surface receptors. In recent decades, small non-coding microRNAs (miRNAs) have been shown to exert an additional layer of control over gene expression and have been demonstrated to act as critical gatekeepers at various checkpoints during the tightly regulated B cell development.
Forensische RNA-Analyse–Möglichkeiten und Perspektiven
Authors: Courts Cornelius 
Published: May 2024

AbstractRNA analysis is used to contextualize biological traces in forensic investigations by assigning biological trace material and components of mixed stains containing different body fluids to particular tissues. While RNA based forensic body fluid and organ tissue identification is already a mature and robust method that is routinely used in forensic casework, there is still active ongoing research exploring the potential of forensic RNA analysis to investigate even more contextual aspects of forensic relevance.
Neuer Standard-Abbautests für synthetische Phosphonate
Authors: Riedel Ramona , Braun Burga , Böllmann Jörg , Martienssen Marion 
Published: May 2024

AbstractThe evaluation of the ready biodegradation of organic substances is usually carried out using the standardised OECD test methods 301 (A–F). However, this can become a great challenge for substances such as aminophosphonates which are chemically imbalanced and do not automatically promote microbial growth in standardized tests. Here, we report on the development of a standardized batch test that is suitable for phosphonates and overcomes important obstacles of the test methods above-mentioned.