bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal quantitative proteomics ofSaccharomyces cerevisiaein response to a nonlethal concentration of furfural
Authors: Lin Feng‐Ming , Tan Ying , Yuan Ying‐Jin 
Published: October 2009

AbstractFurfural, one of the main inhibitory compounds in lignocellulosic hydrolytes, inhibits the growth and ethanol production rate of yeast. To get a global view of the dynamic expression pattern of proteins inSaccharomyces cerevisiaeduring the fermentation with the introduction of 8 g/L furfural, the protein samples were taken before the addition of furfural, during the initial phase of furfural conversion and immediately after the conversion of furfural for comparative proteomic analysis with iTRAQ on a LC‐ESI‐MS/MS instrument. A comparison of the temporal expression pattern of 107 proteins related to protein synthesis between the reference cultures and the furfural‐treated cultures showed that a temporal downregulation of these proteins was retarded after the addition of furfural. The expression levels of 20 enzymes in glucose fermentation and 5 enzymes in the tricarboxylic acid cycle were reduced by furfural, with notably delayed temporal downregulations of Glk1p, Tdh1p, Eno1p and Aco1p, which is correlated to the reduced ethanol formation rate and glucose consumption rate by 66.7 and 60.4%, respectively. In the presence of furfural, proteins catalyzing the upper part of the super pathway of sulfur amino acid biosynthesis were repressed at all time points, which is related to the inhibited growth of furfural‐treated yeast. The expressions of 18 proteins related to stress response showed increased trends, including several highly induced heat shock proteins and proteins related to cellular signaling pathways.
Proteomic profiling in distinct cellular compartments of tumor cells reveals p53‐dependent upregulation of S100A6 upon induction of telomere dysfunction
Authors: Zimmermann Stefan , Biniossek Martin L. , Maurer Christian , Münzer Patrick , Pantic Milena , Veelken Hendrik , Martens Uwe M. 
Published: October 2009

AbstractTelomere dysfunction is evoking a DNA damage response which leads to replicative senescence or apoptosis. Tumor cells feature telomerase, a ribonucleoprotein complex counteracting telomere shortening and proliferation limitation as a prerequisite of immortal cell growth. Recently, we demonstrated the effects of telomerase inhibition on the proteome of tumor cell clones in whole cell lysates by SELDI‐TOF‐MS profiling and MS/MS protein identification (Zimmermann et al., Proteomics 2009, 9, 521–534). We continued proteomic analyses of such clones after telomerase‐inhibition using fractionation of cellular compartments. Among the differentially expressed peaks found in different fractions, a cytoplasmic 10.1 kDa protein upregulated in telomerase‐inhibited clones (p<0.0001) was identified by nanoflow‐HPLC‐MS/MS as S100A6. S100A6 upregulation was confirmed by immunoblotting in telomerase‐inhibited HCT‐116, A‐549, and NCI‐H460 clones. S100A6 and other proteins involved in telomere dysfunction were further analyzed in derivative p53−/− and p21−/− HCT‐116 cell lines indicating an overall reduced number of significant changes in these lines compared to wild type HCT‐116 cells. In addition, post‐translational modification of S100A6 was demonstrated with a potential role in mediating the cellular response to telomere dysfunction. In conclusion, proteomic profiling in distinct cellular compartments led to the identification of a novel p53‐dependent biomarker of telomere dysfunction, S100A6.
LuMPIS – A modified luminescence‐based mammalian interactome mapping pull‐down assay for the investigation of protein–protein interactions encoded by GC‐low ORFs
Authors: Vizoso Pinto Maria Guadalupe , Villegas Josefina Maria , Peter Jan , Haase Rudolf , Haas Jürgen , Lotz Amelie Sophia , Muntau Ania Carolina , Baiker Armin 
Published: October 2009

AbstractThe GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC‐rich coding sequences of a certain protein are used. In order to study protein–protein interactions in Varicella zoster virus, a GC‐low herpesvirus, we have developed a novel luminescence‐based maltose‐binding protein pull‐down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC‐low ORFs in mammalian expression systems.
Identification of molecular targets for selective elimination of TRAIL‐resistant leukemia cells. From spots to in vitro assays using TOP15 charts
Authors: Petrak Jiri , Toman Ondrej , Simonova Tereza , Halada Petr , Cmejla Radek , Klener Pavel , Zivny Jan 
Published: October 2009

AbstractThe resistance of malignant cells to chemotherapy calls for the development of novel anti‐cancer drugs. TNF‐related apoptosis‐inducing ligand (TRAIL) is a pro‐apoptotic cytokine, which selectively induces apoptosis in malignant cells. We derived two TRAIL‐resistant HL‐60 subclones, HL‐60/P1 and HL‐60/P2, from a TRAIL‐sensitive HL‐60 acute promyelocytic leukemia cell line. To identify therapeutically exploitable “weaknesses” of the TRAIL‐resistant leukemia cells that could be used as molecular targets for their elimination, we performed proteomic (2‐DE) analysis and compared both TRAIL‐resistant subclones with the original TRAIL‐sensitive HL‐60 cells. We identified over 40 differentially expressed proteins. To significantly narrow the lists of candidate proteins, we excluded proteins that are known to be often differentially expressed, regardless of experiment type and tissue (the so‐called “TOP15” proteins). Decreased expression of DNA replication and maintenance proteins MCM7 and RPA32 in HL‐60/P1 cells, and the marked down‐regulation of enzyme adenosine deaminase in HL‐60/P2 cells, suggests increased sensitivity of these cells to DNA‐interfering drugs, and adenosine and its homologues, respectively. In a series of in vitro assays, we confirmed the increased toxicity of etoposide and cisplatin to TRAIL resistant HL‐60/P1 cells, and adenosine and vidarabine to HL‐60/P2, compared with TRAIL‐sensitive HL‐60 cells.
Improved method of phosphopeptides enrichment using biphasic phosphate‐binding tag/C18 tip for versatile analysis of phosphorylation dynamics
Authors: Nabetani Takuji , Kim Yeon‐Jeong , Watanabe Masaki , Ohashi Yoko , Kamiguchi Hiroyuki , Hirabayashi Yoshio 
Published: October 2009

AbstractA number of factors including low stoichiometry of phosphorylation, ion suppression, and reduced peptide backbone fragmentation interfere with precise identification of proteins in phosphoproteomic analysis by MS. Therefore, enrichment of phosphopeptides is an important process for subsequent mass spectrometric analysis. Here, we have developed a simple and efficient method for phosphopeptides enrichment, which employs a biphasic phosphate‐binding tag (Phos‐tag)/C18 tip consisting of overlaid Phos‐tag on the C18 resin in a pipet tip. The improvement in selectivity for phosphopeptides was achieved by using a 40% ACN solution under the phosphopeptides binding conditions. We also assessed the adequacy of Phos‐tag/C18 tip for quantitative phosphoproteomic analysis using the iTRAQ technology. After protein digestion and subsequent iTRAQ labeling, interfering substances including excess iTRAQ reagent were directly removed by Phos‐tag/C18 tip in a single step. Applying this method, phosphoproteomic analysis of HeLa cells stimulated with tumor necrosis factor ‐α was rapidly and successfully achieved.
Epigenetics to proteomics: From yeast to brain
Authors: Bhargava Purnima 
Published: October 2009

AbstractBrain is the most complex and least understood organ of the body. Recent research suggests that epigenetics of the brain may be behind the complex functions of this master organ. Yeast, the simplest eukaryote, had been the model for studying the complex physiology of higher eukaryotes, including humans. Current depth in understanding of mechanisms of gene regulation has been possible mainly because of the knowledge acquired by epigenetic studies on yeast while the research on the biochemistry and physiology of the brain has been tremendously benefitted by proteomic studies. The independent advances of research in both these fields are now converging. As the current understanding of epigenetics can be applied to understand the mysteries of normal brain function as well as various diseases, modern proteomic approaches can help find new therapeutic targets.
Proteomic DIGE analysis of the mitochondria‐enriched fraction from aged rat skeletal muscle
Authors: O'Connell Kathleen , Ohlendieck Kay 
Published: October 2009

AbstractSkeletal muscle aging is associated with a loss in tissue mass and contractile strength, as well as fiber type shifting and bioenergetic adaptation processes. Since mitochondria represent the primary site for energy generation via oxidative phosphorylation, we investigated potential changes in the expression pattern of the mitochondrial proteome using the highly sensitive DIGE approach. The comparative analysis of the mitochondria‐enriched fraction from young adult versus aged muscle revealed an age‐related change in abundance for 39 protein species. MS technology identified the majority of altered proteins as constituents of muscle mitochondria. An age‐dependent increase was observed for NADH dehydrogenase, the mitochondrial inner membrane protein mitofilin, peroxiredoxin isoform PRX‐III, ATPase synthase, succinate dehydrogenase, mitochondrial fission protein Fis1, succinate‐coenzyme A ligase, acyl‐coenzyme A dehydrogenase, porin isoform VDAC2, ubiquinol‐cytochrome c reductase core I protein and prohibitin. Immunoblotting, enzyme testing and confocal microscopy were used to validate proteomic findings. The DIGE‐identified increase in key mitochondrial elements during aging agrees with the concept that sarcopenia is associated with a shift to a slower contractile phenotype and more pronounced aerobic‐oxidative metabolism. This suggests that mitochondrial markers are reliable candidates that should be included in the future establishment of a biomarker signature of skeletal muscle aging.
Fifty years of the integrated control concept: moving the model and implementation forward in Arizona
Authors: Naranjo Steven E , Ellsworth Peter C 
Published: October 2009

AbstractFifty years ago, Stern, Smith, van den Bosch and Hagen outlined a simple but sophisticated idea of pest control predicated on the complementary action of chemical and biological control. This integrated control concept has since been a driving force and conceptual foundation for all integrated pest management (IPM) programs. The four basic elements include thresholds for determining the need for control, sampling to determine critical densities, understanding and conserving the biological control capacity in the system and the use of selective insecticides or selective application methods, when needed, to augment biological control. Here we detail the development, evolution, validation and implementation of an integrated control (IC) program for whitefly, Bemisia tabaci (Genn.), in the Arizona cotton system that provides a rare example of the vision of Stern and his colleagues. Economic thresholds derived from research‐based economic injury levels were developed and integrated with rapid and accurate sampling plans into validated decision tools widely adopted by consultants and growers. Extensive research that measured the interplay among pest population dynamics, biological control by indigenous natural enemies and selective insecticides using community ordination methods, predator:prey ratios, predator exclusion and demography validated the critical complementary roles played by chemical and biological control. The term ‘bioresidual’ was coined to describe the extended environmental resistance from biological control and other forces possible when selective insecticides are deployed. The tangible benefits have been a 70% reduction in foliar insecticides, a >$200 million saving in control costs and yield, along with enhanced utilization of ecosystem services over the last 14 years. Published in 2009 by John Wiley & Sons, Ltd.
Research on nickel morphology of dependence on the microwave‐assisted polyol method
Authors: Xiansong Liu , Huamin Gao , Shixing Qiu , Luguo Chen , Yuxing Dend , Dan Zhou , Xiaobing Xu 
Published: October 2009

AbstractThe structures, morphology and magnetic properties of the ultrafine Ni nanoparticles prepared by microwave‐assisted polyol method was investigated, using X‐ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM), respectively. The XRD results suggested that Ni nanoparticles with an fcc structure were successfully synthesized. It was interesting to note that the Ni nanoparticles are ideally spherical, which were examined by the TEM. As immersed in different solutions, we can get different shape nickel particles with different conditions. Especially, the experimental results indicated that nanohair nickel particles were ferromagnetic.
Boron, nitrogen and phosphorous substitutionally doped single‐wall carbon nanotubes studied by resonance Raman spectroscopy
Authors: Maciel I. O. , Campos‐Delgado J. , Pimenta M. A. , Terrones M. , Terrones H. , Rao A. M. , Jorio A. 
Published: October 2009

AbstractSubstitutional doping on single‐wall an on multi‐wall carbon nanotubes is possible by adding new atoms during the growing process. In this work we analyze the changes in the Raman spectra of SWNT samples substitutionaly doped with boron, nitrogen, and phosphorous. We find that small amounts of dopants are not enough to change the frequency of the tangential G mode, but there is a doping‐dependent shift in ωG when the P‐doped SWNTs are measured at high laser power, indicating that doping can change the thermal properties of SWNT bundles. This result seems to hold also for B‐doped samples, but not for N‐doped SWNTs, showing a different behavior for donors (P‐ and N‐ doping). The ID/IG ratio analysis to provide information about the doping level in the SWNT samples is also discussed.
Positron source Ge‐68 through copper and bronze irradiated by carbon ions
Authors: Srivastava Subodh , Agrawal Shweta , Singh M. , Vijay Y. K. , Bhagwat P. V. , Sharma S. C. 
Published: October 2009

AbstractThis communication reports on the formation of radioactive germanium isotope (G68) in copper (Cu) and bronze irradiated with Carbon ions (70MeV, 700 nA for 20 h) at the pelletron facility at TIFR, Mumbai. The irradiated targets were analyzed by gamma spectroscopy using HPGe detector and end point energy using GM counter. By analysis of gamma spectrum, it has been observed that Ge68 isotope along with some other isotopes like Se75 (119 days), Ge66 (2.26 days), Ga74 (8.12 minutes), As69 (15.2 minutes), Co60 (1925days), Zn65 (243days), Ga68 (67.7 minutes) were formed as estimated by PACE Code calculations. These results show that both Cu and bronze have contributed to Ge68 production but the irradiated bronze is found to form a good positron emitter in comparison to that of irradiated copper. The beta end point energy of Ge68 estimated by GM counter has been found to be 1.80 MeV. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Positron annihilation study of recrystallization behaviour in Zr2.5%Nb alloy
Authors: Mulki S. , Pujari P. K. , Srivastava D. , Samajdar I. , Dey G. K. , Hiwarkar V. D. , Sharma S. 
Published: October 2009

AbstractIn CANDU reactors Zr 2.5%Nb alloy is used as pressure tube material due to low neutron absorption cross section, a high resistance to corrosion in water, high strength and good creep resistance. The two phase alloy consists of α‐fcc and β‐bcc. The recrystallization behavior of Zr2.5Nb strongly depends on morphology and distribution of second phase. In this study, the material was deformed to 60% and then was recrystallized in α + β region for different duration (i.e. 2 minutes to 14 days at 700 °C). The recrystallized microstructure was characterized by Orientation Imaging Microscopy (OIM). The evolution of microstructure was examined by Positron Lifetime Spectroscopy and Doppler Broadening measurement for each heat treated samples. Results from positron annihilation studies and other microstructural examination have been used to rationalize the recrystallization aspects, defects and β‐phase distribution. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Electrical resistivity peculiarities and positron lifetime in annealed CdO
Authors: Sarkar A. , Chattopadhyay S. , De Udayan 
Published: October 2009

AbstractDifferently heat‐treated polycrystalline cadmium oxide (CdO) samples have been studied using room temperature (RT) resistivity, positron annihilation lifetime (PAL) and ultraviolet visible (UV‐Vis) optical absorption measurements. A small increase of the resistivity for 275 °C annealing (with respect to the other samples) and a large reduction of the same for 810 °C annealing have been observed. PAL investigation shows presence of defects, presumably Cd+O divacancy (VCd+O) clusters, in all the samples. Gradual defect recovery takes place with increasing annealing temperature. A clear defect recovery stage has been found above 400 °C annealing from where drastic fall of resistivity occurs. The optical band gaps (direct and indirect) of the 125 °C annealed sample comes out to be 1.89 eV and 0.79 eV. The indirect band gaps of the samples show anomalous variation due to 275 °C annealing which is also consistent with resistivity anomaly. To our knowledge, this is the first positron spectroscopic investigation on the CdO system. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Dance with the one that brought you? Venture capital firms and the retention of founder‐CEOs
Authors: Pollock Timothy G. , Fund Bret R. , Baker Ted 
Published: October 2009

AbstractWe consider how a venture capital firm's perceived uncertainty in new and uncertain industry environments affects its decisions to retain founder‐CEOs at companies they take public. We further consider how the human capital of the founder‐CEO, the overall experience of the venture capital firm (VCF), and the VCF's specific experience with the new industry moderate the relationship between industry‐based uncertainty and founder‐CEO retention. We explore these issues in the context of 340 venture capital firm investments in Internet sector start‐ups that went public from 1995 to 2000. We find evidence that industry‐based uncertainty decreases the likelihood of founder‐CEO retention, that founder‐CEO human capital and VCF Internet‐sector experience decreases the effects of these uncertainties on founder‐CEO retention for business‐to‐business (B2B) firms, but increases them for business‐to consumer (B2C) firms, and that VCF age further decreases the likelihood that the founder‐CEOs of B2C firms will be retained. Copyright © 2009 Strategic Management Society.
Understanding the relationship between entrepreneurial orientation and strategic learning capability: an empirical investigation
Authors: Anderson Brian S. , Covin Jeffrey G. , Slevin Dennis P. 
Published: October 2009

AbstractThis research explores the relationship between strategic learning capability—a firm's proficiency at generating, and then acting on, strategic knowledge—and entrepreneurial orientation (EO). While theory posits the inevitably of building strategic learning capability from behaving entrepreneurially, there is little empirical research to validate this proposition and even less understanding of how and why EO contributes to strategic learning capability. Empirical results from 110 manufacturing firms confirm the direct effect of EO on strategic learning capability, and support is found for three constructs—structural organicity, market responsiveness, and strategy formation mode—that fully mediate the EO‐strategic learning capability relationship. Copyright © 2009 Strategic Management Society.
Existence, uniqueness and stability of equilibrium states for non-uniformly expanding maps
Authors: Varandas Paulo , Viana Marcelo 
Published: October 2009

We prove existence of finitely many ergodic equilibrium states for a large class of non-uniformly expanding local homeomorphisms on compact metric spaces and Hölder continuous potentials with not very large oscillation. No Markov structure is assumed. If the transformation is topologically mixing there is a unique equilibrium state, it is exact and satisfies a non-uniform Gibbs property. Under mild additional assumptions we also prove that the equilibrium states vary continuously with the dynamics and the potentials (statistical stability) and are also stable under stochastic perturbations of the transformation.

Résumé
Nous prouvons l'existence d'un nombre fini d'états d' équilibre ergodiques pour une classe assez grande d'homéomorphismes locaux non-uniformément dilatants sur des espaces métriques compacts et pour les potentiels de Hölder continus à oscillation pas trop grande. Aucune structure de Markov n'est supposée. Si la transformation est topologiquement mélangeante alors il existe un unique état d' équilibre, il est une mesure exacte et vérifie une propriété de Gibbs non-uniforme. Avec quelques hypothèses supplémentaires, nous prouvons aussi que les états d' équilibre varient de façon continue avec la dynamique et le potentiel (stabilité statistique) et sont également stables sous des perturbations stochastiques de la transformation.
Childlessness and intergenerational transfers: what is at stake?
Authors: KOHLI MARTIN , ALBERTINI MARCO 
Published: October 2009

ABSTRACTIn this introductory article for the special issue onChildlessness and Intergenerational Transfers, we first discuss the prior research literature and then overview the presented contributions. Up to now, childless older adults have been treated for the most part as both homogeneous and a problematic group. This does not do justice to the different pathways to childlessness: there are those who actively forgo having children, those who defer births so long that they involuntarily become childless, and those who are not fecund or lack a partner. It also neglects the changing social profile of the childless,e.g. the shift from less educated to more highly-educated women. Most importantly, it fails to recognise what childless older people give to others. The studies presented here aim to redress these two deficits in previous research. They examine how the consequences of childlessness are mediated by the pathways to and motivations for being childless and by factors such as gender, education and marital history, and they also examine what childless older adults provide to their families and to society at large. Such adults establish strong linkages with next-of-kin relatives, invest in non-family networks, and participate in voluntary and charitable activities, and broadly do so to a greater extent than older people with surviving children.
Record of the thirteenth conference of the International Society of Anglo-Saxonists, at the Institute of English Studies, University of London, 30 July to 4 August 2007
Authors: Swan Mary 
Published: October 2009

I The general theme of the conference was ‘Anglo-Saxon Traces: Her mon mæg giet gesion hiora swæð’. The conference was attended by 196 people.The following papers were delivered:Anton Scharer, ‘Objects of Royal Representation (in England and on the Continent)’Jennifer O'Reilly ‘The Topography of Islands in Bede's Historia ecclesiastica’Diarmuid Scully, ‘Among the Ruins: Bede and his Sources on the Geography and Built Environment of Roman Britain’Joshua Westgard, ‘Traces of Bede?: the Moore Continuations and the Transmission of Bede's Historia ecclesiastica’Richard North, ‘Revenue and Real Estate: Archbishop Wulfred and the Strange Case of Cynehelm’