bluuebunny/crossref_metadata_2025_split huggingface dataset:
The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors
Authors: Lozano Casasbuenas Daniela , Kortebi Ines , Gora Charles , Scott Erica Y. , Gomes Celeste , Oliveira Markley Silva , Sharma Tanvi , Daniele Emerson , Olfat Arman , Gibbs Rachel , Yuzwa Scott A. , Gilbert Emily A. , Küry Patrick , Wheeler Aaron R. , Lévesque Martin , Faiz Maryam 
Published: June 2024

AbstractAstrocytes that reside in superficial (SL) and deep cortical layers have distinct molecular profiles and morphologies, which may underlie specific functions. Here, we demonstrate that the production of SL and deep layer (DL) astrocyte populations from neural progenitor cells in the mouse is temporally regulated. Lineage tracking following in utero and postnatal electroporation with PiggyBac (PB) EGFP and birth dating with EdU and FlashTag, showed that apical progenitors produce astrocytes during late embryogenesis (E16.5) that are biased to the SL, while postnatally labeled (P0) astrocytes are biased to the DL. In contrast, astrocytes born during the predominantly neurogenic window (E14.5) showed a random distribution in the SL and DL. Of interest, E13.5 astrocytes birth dated at E13.5 with EdU showed a lower layer bias, while FT labeling of apical progenitors showed no bias. Finally, examination of the morphologies of “biased” E16.5‐ and P0‐labeled astrocytes demonstrated that E16.5‐labeled astrocytes exhibit different morphologies in different layers, while P0‐labeled astrocytes do not. Differences based on time of birth are also observed in the molecular profiles of E16.5 versus P0‐labeled astrocytes. Altogether, these results suggest that the morphological, molecular, and positional diversity of cortical astrocytes is related to their time of birth from ventricular/subventricular zone progenitors.
A case of malignant pheochromocytoma with neurofibromatosis type 1 having difficulty in differentiating spinal tumor
Authors: Segawa Kohei , Yamamoto Yoshiyuki , Kato Taigo , Hatano Koji , Kakuta Yoichi , Kawashima Atsunari , Fukuhara Shinichiro , Nonomura Norio 
Published: June 2024

IntroductionNeurofibromatosis type 1 is a hereditary condition often associated with pheochromocytomas but rarely with malignant pheochromocytomas. Neurofibromatosis type 1 is often associated with bone lesions, which complicates the distinction between malignant and benign tumors.Case presentationA 46‐year‐old man with a medical history of neurofibromatosis type 1 presented with right abdominal pain. Computed tomography revealed a right adrenal tumor, and metaiodobenzylguanidine scintigraphy showed accumulation in the right adrenal gland and thoracic vertebrae. He was diagnosed with pheochromocytoma, and a right adrenalectomy was performed. After surgery, a bone biopsy was conducted on the spinal lesion, confirming metastasis of pheochromocytoma, prompting irradiation. After that, lung and liver metastases emerged, and chemotherapy with cyclophosphamide, vincristine, and dacarbazine was initiated; however, the disease progressed, and he died 11 months after surgery.ConclusionWe report a case of malignant pheochromocytoma associated with neurofibromatosis type 1 in which bone metastasis was difficult to diagnose.
Characterization of ERα Signaling to Cell Proliferation Induced by Chronic and Pulsatile E2 Stimulation in 2D and 3D Cell Cultures
Authors: Fiocchetti Marco , Raimondi Serena , Bastari Giovanna , Bartoloni Stefania , Marino Maria , Acconcia Filippo 
Published: June 2024

ABSTRACT17β‐estradiol is a hormone that plays a vital role in human physiology. It acts through estrogen receptors, specifically estrogen receptor α and estrogen receptor β, and its action is determined by the pulsatile secretion in the bloodstream. 17β‐estradiol affects cell proliferation, and dysregulation of 17β‐estradiol:estrogen receptor α signaling contribute to the development of breast cancer. Previous research on 17β‐estradiol:estrogen receptor α signaling has primarily used two‐dimensional cell cultures, which do not fully recapitulate the complexity of tumors that exist in a three‐dimensional environment and do not consider the pulsatile nature of this hormone. To address these limitations, we studied 17β‐estradiol:estrogen receptor α signaling in cell proliferation using both two‐dimensional and three‐dimensional breast cancer cell culture models under continuous and pulsatile stimulation conditions. Results revealed that breast cancer cells grown in an alginate‐based three‐dimensional matrix exhibited similar responsiveness to 17β‐estradiol compared with cells grown in conventional two‐dimensional culture plates. 17β‐estradiol induced the expression of proteins containing estrogen response element in the three‐dimensional model. The efficacy of the antiestrogen drugs fulvestrant (ICI182,280) and 4OH‐tamoxifen was also demonstrated in the three‐dimensional model. These results support the use of the three‐dimensional culture model for studying tumor response to drugs and provide a more realistic microenvironment for such studies. Furthermore, the study revealed that a brief 5‐min exposure to 17β‐estradiol triggered a physiological response comparable with continuous hormone exposure, suggesting that the cellular response to 17β‐estradiol is more important than the continuous presence of the hormone. In conclusion, the study demonstrates that the alginate‐based three‐dimensional culture model is suitable for studying the effects of 17β‐estradiol and antiestrogen drugs on breast cancer cells, offering a more realistic representation of tumor‐microenvironment interactions. The results also highlight the importance of considering the physiological importance of the temporal dynamics in studying 17β‐estradiol signaling and cellular responses.
Limitations of the knee society score in kinematically aligned total knee arthroplasty
Authors: Leica Alexandra , Sava Manuel‐Paul , Khan Zainab‐Aqeel , Hügli Rolf W. , Hirschmann Michael T. 
Published: June 2024

AbstractPurposeThe Knee Society Scoring System (KSS) is a frequently used outcome score which quantifies functional patients' outcomes before and after total knee arthroplasty (TKA). Several problems arise when trying to implement KSS for obtaining postoperative outcomes after more personalised aligned TKAs. Scoring for valgus femorotibial angle (FTA) intervals outside moderate ranges is often poorly explained, the specific version of KSS used for outcome collection is frequently unclear and the exact measuring methods are typically not described in the literature. The aims of this systematic review were to investigate the latest user practice, the application of KSS and its limitations after kinematically aligned (KA) TKA.MethodsA systematic literature search following PRISMA guidelines was conducted on PubMed, Embase, Medline and Scopus to identify potentially relevant articles for this review, published from the beginning of January 2013 until the end of January 2023. Broad Mesh terms such as ‘kinematic alignment’, ‘total knee arthroplasty’ and ‘knee society score’ were used for building search strategy in each database accordingly. Articles reporting postoperative values of the objective surgeon‐assessed KSS after KA TKA or KA and mechanically aligned TKA were included. For assessing included randomised control trials (RCTs), an Agency for Healthcare Research and Quality's design‐specific scale for assessing RCTs was used. The non‐RCTs were assessed by using the Joanna Briggs Institute Critical Appraisal Tool. The Ottawa–Newcastle Score system was also used. Studies were additionally evaluated for their radiological methodology by using a five‐question checklist (Radiological Assessment Qualit criteria).ResultsThe initial search identified 167 studies, of which 129 were considered for screening. Ten studies reporting outcomes after KA TKA did not use the objective surgeon‐assessed part of KSS for clinical outcome measurement, and 30 studies reporting outcomes after KA TKA did not use KSS at all for clinical and/or functional outcomes. From the 10 included studies, only six have used the latest KSS score (2011), the rest using its 1989 variant; and out of these six studies, only two presented values of the FTA, which is needed for calculating the KSS's ‘alignment’ subcomponent, the rest presenting hip–knee–ankle angle (HKA) values. Additionally, when converting these HKA values to FTA intervals, the authors of this systematic review found that KA TKA FTA intervals display limits, which tend to be outside the ‘well‐scored’ KSS anatomical alignment interval.ConclusionThe inconsistent and nonstandardised use of the surgeon‐assessed KSS across studies reviewed compromises assessment reliability and patient outcome scores. To enhance precision and comparability, it is crucial to standardise the KSS application, incorporating personalised alignment strategies for more accurate patient evaluations.Level of EvidenceLevel III.
Evaluation of monkeypox‐ and vaccinia‐virus neutralizing antibodies before and after smallpox vaccination: A sero‐epidemiological study
Authors: Marchi Serena , Piccini Giulia , Cantaloni Paolo , Guerrini Noemi , Zannella Roberta , Coluccio Rosa , Benincasa Linda , Solfanelli Niccolò , Remarque Edmond J. , Viviani Simonetta , Kistner Otfried , Temperton Nigel , Montomoli Emanuele , Manenti Alessandro , Trombetta Claudia Maria 
Published: June 2024

AbstractSince May 2022, several countries outside of Africa experienced multiple clusters of monkeypox virus (MPXV)‐associated disease. In the present study, anti‐MPXV and anti‐vaccinia virus (VACV) neutralizing antibody responses were evaluated in two cohorts of subjects from the general Italian population (one half born before the WHO‐recommended end of smallpox vaccination in 1980, the other half born after). Higher titers (either against MPXV or VACV) were observed in the cohort of individuals born before the interruption of VACV vaccination. An association between VACV and MPXV antibody levels was observed, suggesting that the smallpox vaccination may confer some degree of cross‐protection against MPXV infection. Results from this study highlight low levels of immunity toward the assessed Orthopoxviruses, especially in young adults, advocating the introduction of a VACV‐ or MPXV‐specific vaccine in case of resurgence of monkeypox disease outbreaks.
Integrating the science of epigenetics with existential–humanistic counseling to increase wellness with Black males
Authors: Burt Isaac , Tosado Luis A. 
Published: June 2024

AbstractThe recent emphasis on racial unrest and systemic inequality perpetuated by COVID‐19 has made the counseling profession seek more appropriate methods for clients, especially with Black males. Epigenetics, a science examining how behaviors and environment intersect to impact gene expression, could serve as this tool. This article advocates for providing therapy through an epigenetics framework, integrated with existential–humanism to give counselors the tools necessary to work with contemporary Black male clients.
Association between celiac disease and pneumococcal infections in hospitalized pediatric patients in the United States
Authors: Jang Janet , Krishnamurthy Jayasree , Nylund Cade M. 
Published: June 2024

AbstractObjectiveCeliac disease (CD) is an immune‐mediated enteropathy that is associated with pneumococcal infections in adults. The objective of this study is to evaluate the association between CD and pneumococcal infections in hospitalized pediatric patients in the United States (US).Study DesignThe triennial Healthcare Cost and Utilization Project Kids' Inpatient Database was used in a retrospective analysis of children hospitalized in the US from 1997 to 2019. Billing codes were used to define patients with CD who were admitted with Streptococcus pneumoniae speciated infections or an infection commonly caused by S. pneumoniae. A multivariable logistic regression model was used to quantify increased odds of various types of infections for patients with CD.ResultsAmong 55,080,914 pediatric hospital admissions, 15,412 were identified with CD, and 1,722,872 were admitted with the specified infections. CD was associated with both pneumococcus speciated infections (odd ratio [OR], 2.16, 95% confidence interval [CI], 1.38–3.38) and infections commonly caused by S. pneumoniae (OR, 1.78; 95% CI, 1.61−1.96): pneumonia (OR, 1.70; 95% CI, 1.53−1.89), sinusitis (OR, 2.41, 95% CI, 1.76−3.30), and bacteremia (OR, 2.12; 95% CI, 1.56−2.88). Patients with CD had a significantly longer length of stay (p < 0.001) and a greater cost of hospitalization (p < 0.001) with pneumococcus associated infections.ConclusionsCD is associated with an increased risk of both pneumococcus speciated and pneumococcus‐associated infections requiring hospitalization. CD admissions are associated with longer hospital stays and higher costs without increased risk of death. Routine pneumococcal vaccinations are strongly recommended for pediatric patients with CD.
Lateral extra‐articular tenodesis does not enhance ACL graft healing, however, does reduce graft tunnel widening
Authors: Jacob George , Lim Yoong Ping , Brito Cristian Ayet , Parker David A. 
Published: June 2024

AbstractPurposeThe study hypothesized that the addition of lateral extra‐articular tenodesis (LEAT) in anterior cruciate ligament reconstruction (ACLR) had a significant effect on ACL graft healing.MethodsA total of 80 patients were divided into two cohorts matched for gender, age, body mass index, time from surgery to post‐operative MRI and graft diameter. Forty patients underwent ACL reconstruction alone, while 40 underwent ACLR in addition to LEAT. Patients underwent a magnetic resonance imaging scan at 12 months post‐surgery; tunnel apertures were measured using multiplanar reformation, graft healing was assessed using signal‐to‐noise quotient (SNQ) in three regions of interest and finally graft maturity and integration were classified using the Howell and Ge scale, respectively. In addition, clinical evaluation and patient‐reported outcome measures were collected.ResultsThe mean femoral tunnel widening at 12 months post‐surgery was 39.8 ± 14.0% in the ACLR + LEAT group and 55.2 ± 12.7% in the ACLR alone group (p < 0.05). The mean tibial tunnel widening was 29.3 ± 12.7% in the ACLR + LEAT group and 44.4 ± 12.1% in the ACLR group (p < 0.05). The mean adjusted graft SNQ was 9.0 ± 14.9 in the ACLR + LEAT group and 9.5 ± 11.4 in the ACLR group (n.s.).ConclusionAt 1 year post‐operatively, we noted significantly less femoral and tibial tunnel widening in the ACLR + LEAT group. LEAT did not result in a statistically significant effect on graft healing.Level of EvidenceLevel III.
Thermal Enhanced Energy Transfer and High Thermal Sensitivity of Sb3+ Doped Cs2KYbCl6 Rare Earth Double Perovskites
Authors: Wang Yanchao , Hou Haowen , Bai Yunlong , Zou Bingsuo , Zeng Ruosheng 
Published: June 2024

AbstractRare‐earth based double perovskites (DPs) have attracted much attention due to stable, efficient, and unique luminescence, and wide applications in many optoelectronic fields. However, their weak near‐infrared (NIR) emission and poor anti‐thermal quenching severely limit the further applications. Herein, Sb3+‐doped Cs2KYbCl6 DP with cyan self‐trapped exciton (STE) and NIR emission is prepared by a solvothermal method, and the separate photoluminescence quantum yields from STE and NIR emission reached ≈26.1% and 43.8%, respectively. More importantly, Sb3+ not only contributes to the absorption and energy transfer but also helps to establish an effective thermally enhanced energy transfer channel through self‐trapping state, resulting in the NIR emission with superior anti‐thermal quenching resistance. The thermal sensitivity of luminescence intensity ratio (LIR) of I1010 nm/I505 nm (I is the intensity of PL) and time‐resolved temperature sensor reach 21.4 and 13.6% K−1, respectively, which are ahead of most temperature sensing materials. Furthermore, it is found that the material exhibited advanced multifunctional applications in LED lighting, flexible luminescent thin film, and NIR imaging. This work provides in‐depth understanding on photophysical mechanisms of rare‐earth luminescent materials and references for designing high‐performance materials.
HDAC inhibitor regulates the tumor immune microenvironment via pyroptosis in triple negative breast cancer
Authors: Yang Xue , Cui Xiaoqing , Wang Ge , Zhou Mengying , Wu Yonglin , Du Yaying , Li Xingrui , Xu Tao 
Published: June 2024

AbstractPyroptosis, an inflammatory form of cell death, promotes the release of immunogenic substances and stimulates immune cell recruitment, a process, which could turn cold tumors into hot ones. Thus, instigating pyroptosis in triple‐negative breast cancer (TNBC) serves as a viable method for restoring antitumor immunity. We analyzed the effects of Histone Deacetylase Inhibitors (HDACi) on TNBC cells using the Cell Counting Kit‐8 and colony formation assay. Apoptosis and lactate dehydrogenase (LDH) release assays were utilized to determine the form of cell death. The pyroptotic executor was validated by quantitative real‐time polymerase chain reaction and western blot. Transcriptome was analyzed to investigate pyroptosis‐inducing mechanisms. A subcutaneously transplanted tumor model was generated in BALB/c mice to evaluate infiltration of immune cells. HDACi significantly diminished cell proliferation, and pyroptotic “balloon”‐like cells became apparent. HDACi led to an intra and extracellular material exchange, signified by the release of LDH and the uptake of propidium iodide. Among the gasdermin family, TNBC cells expressed maximum quantities of GSDME, and expression of GSDMA, GSDMB, and GSDME were augmented post HDACi treatment. Pyroptosis was instigated via the activation of the caspase 3‐GSDME pathway with the potential mechanisms being cell cycle arrest and altered intracellular REDOX balance due to aberrant glutathione metabolism. In vivo experiments demonstrated that HDACi can activate pyroptosis, limit tumor growth, and escalate CD8+ lymphocyte and CD11b+ cell infiltration along with an increased presence of granzyme B in tumors. HDACi can instigate pyroptosis in TNBC, promoting infiltration of immune cells and consequently intensifying the efficacy of anticancer immunity.
A multilayer frequency‐selective rasorber for wideband transmission based on harmonic suppression
Authors: Chen Yuxu , Wan GuoBin , Ma Xin , Fu Bin , Jiao Xinhui 
Published: June 2024

AbstractTo break through the limitation of harmonic loss on transmission bandwidth, a multilayer frequency‐selective rasorber (FSR) for wideband transmission is present in this letter. First, the principle of harmonic suppression implemented by a lossy layer is described and analyzed in detail. Second, based on this lossy layer, an absorption–transmission (A–T) rasorber with harmonic suppression, which can generate a wide passband above the lower absorption band, is achieved. And then, by combining with a transmission–absorption (T–A) rasorber, an absorption–transmission–absorption (A–T–A) FSR with a wide and low insertion loss (IL) passband can be obtained. The simulation result shows that the −1‐dB passband ranges from 7.1 to 17.49 GHz with 84.5% relative bandwidth. Finally, a prototype is fabricated and measured to validate the design.
Wideband black‐blood late gadolinium enhancement imaging for improved myocardial scar assessment in patients with cardiac implantable electronic devices
Authors: Gut Pauline , Cochet Hubert , Caluori Guido , El‐Hamrani Dounia , Constantin Marion , Vlachos Konstantinos , Sridi Soumaya , Antiochos Panagiotis , Schwitter Jürg , Masi Ambra , Sacher Frederic , Jaïs Pierre , Stuber Matthias , Bustin Aurélien 
Published: June 2024

AbstractPurposeWideband phase‐sensitive inversion recovery (PSIR) late gadolinium enhancement (LGE) enables myocardial scar imaging in implantable cardioverter defibrillators (ICD) patients, mitigating hyperintensity artifacts. To address subendocardial scar visibility challenges, a 2D breath‐hold single‐shot electrocardiography‐triggered black‐blood (BB) LGE sequence was integrated with wideband imaging, enhancing scar‐blood contrast.MethodsWideband BB, with increased bandwidth in the inversion pulse (0.8–3.8 kHz) and T2 preparation refocusing pulses (1.6–5.0 kHz), was compared with conventional and wideband PSIR, and conventional BB, in a phantom and sheep with and without ICD, and in six patients with cardiac devices and known myocardial injury. ICD artifact extent was quantified in the phantom and specific absorption rate (SAR) was reported for each sequence. Image contrast ratios were analyzed in both phantom and animal experiments. Expert radiologists assessed image quality, artifact severity, and scar segments in patients and sheep. Additionally, histology was performed on the sheep's heart.ResultsIn the phantom, wideband BB reduced ICD artifacts by 62% compared to conventional BB while substantially improving scar‐blood contrast, but with a SAR more than 24 times that of wideband PSIR. Similarly, the animal study demonstrated a considerable increase in scar‐blood contrast with wideband BB, with superior scar detection compared with wideband PSIR, the latter confirmed by histology. In alignment with the animal study, wideband BB successfully eliminated severe ICD hyperintensity artifacts in all patients, surpassing wideband PSIR in image quality and scar detection.ConclusionWideband BB may play a crucial role in imaging ICD patients, offering images with reduced ICD artifacts and enhanced scar detection.
Evaluating cerebrovascular reactivity measured by velocity selective inversion arterial spin labeling with different post‐labeling delays: The effect of fast flow
Authors: Xu Feng , Xu Cuimei , Zhu Dan , Liu Dapeng , Lu Hanzhang , Qin Qin 
Published: June 2024

AbstractPurposeVelocity selective arterial spin labeling (VSASL) quantification assumes that the labeled bolus continuously moves into the imaging voxel during the post‐labeling delay (PLD). Faster blood flow could lead to a bolus duration shorter than the applied PLD of VSASL and cause underestimation of cerebral blood flow (CBF). This study aims to evaluate the performance of velocity‐selective inversion (VSI) prepared arterial spin labeling (ASL) with different PLDs and pseudo‐continuous ASL (PCASL) for quantification of hypercapnia‐induced cerebrovascular reactivity (CVR), using phase‐contrast (PC) MRI as a global reference.MethodsWe compared CVR obtained by VSI‐ASL with PLD of 1520 ms (VSASL‐1520), 1000 ms (VSASL‐1000), and 500 ms (VSASL‐500), PCASL with PLD of 1800 ms (PCASL‐1800), and PC MRI on eight healthy volunteers at two sessions.ResultsCompared with PC MRI, VSASL‐1520 produced significantly lower global CVR values, while PCASL‐1800, VSASL‐1000, and VSASL‐500 yielded more consistent results. The reduced CVR in VSASL‐1520 was more pronounced in carotid territories including frontal and temporal lobes than in vertebral territories such as the occipital lobe. This is largely caused by the underestimated perfusion during hypercapnia due to the reduced bolus duration being less than the PLD.ConclusionAlthough VSASL offers certain advantages over spatially selective ASL due to its reduced susceptibility to delayed ATT, this technique is prone to biases when the ATT is excessively short. Therefore, a short PLD should be employed for reliable perfusion and CVR quantification in populations or conditions with fast flow.