bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of cyclic nucleotide phosphodiesterases 4 in developing rat lung
Authors: Lopez Emmanuel , Jarreau Pierre‐Henri , Zana Elodie , Franco‐Montoya Marie‐Laure , Schmitz Thomas , Evain‐Brion Danièle , Bourbon Jacques , Delacourt Christophe , Méhats Céline 
Published: July 2010

AbstractDuring the perinatal period, lungs undergo changes to adapt to air breathing. The genes involved in these changes are developmentally regulated by various signaling pathways, including the cyclic nucleotide cAMP. As PDE4s are critical enzymes for regulation of cAMP levels, the objective of this study was to investigate PDE4's ontogeny in developing rat lung during the perinatal period. Pulmonary PDE4 activity, PDE4A‐D, PDE4B, and PDE4D variant expression levels, PDE4B and PDE4D protein levels, and PDE4D localization in distal lung were determined. PDE4 activity increased towards term, dropped at birth, and increased thereafter to reach a plateau at the end of the second week of life. PDE4B2 and PDE4D long forms demonstrated a pattern of expression that increased markedly at birth. After birth, PDE4D was expressed in alveolar epithelial and mesenchymal cells. The study, therefore, evidenced striking variations in expression patterns among the PDE4 family that differed from changes in global PDE4 activity. Developmental Dynamics 239:2470–2478, 2010. © 2010 Wiley‐Liss, Inc.
Gene expression in the efferent ducts, epididymis, and vas deferens during embryonic development of the mouse
Authors: Snyder Elizabeth M. , Small Christopher L. , Bomgardner Daniela , Xu Bingfang , Evanoff Ryan , Griswold Michael D. , Hinton Barry T. 
Published: July 2010

AbstractThe tissues of the male reproductive tract are characterized by distinct morphologies, from highly coiled to un‐coiled. Global gene expression profiles of efferent ducts, epididymis, and vas deferens were generated from embryonic day 14.5 to postnatal day 1 as tissue‐specific morphologies emerge. Expression of homeobox genes, potential mediators of tissue‐specific morphological development, was assessed. Twenty homeobox genes were identified as either tissue‐enriched, developmentally regulated, or both. Additionally, ontology analysis demonstrated cell adhesion to be highly regulated along the length of the reproductive tract. Regulators of cell adhesion with variable expression between the three tissues were identified including Alcam, various cadherins, and multiple integrins. Immunofluorescence localization of the cell adhesion regulators POSTN and CDH2 demonstrated cell adhesion in the epithelium and mesenchyme of the epididymis may change throughout development. These results suggest cell adhesion may be modulated in a tissue‐specific manner, playing an important role in establishing each tissue's final morphology. Developmental Dynamics 239:2479–2491, 2010. © 2010 Wiley‐Liss, Inc.
Transient stability output margin estimation based on the energy function method
Authors: Miwa Natsuki , Tanaka Kazuyuki 
Published: July 2010

AbstractIn this paper, a new method of estimating the critical generation margin (CGM) in power systems is proposed based on transient stability diagnostics. The proposed method can directly compute the stability limit output for a given fault based on the transient energy function method (TEF). Since CGM can be directly obtained by the limit output using estimated P‐θ curves and is easy to understand, it is more useful than the conventional critical clearing time (CCT) of the energy function method. The proposed method can also estimate CGM as a negative value, which means instability in the present load profile, and so a negative CGM can be directly used as a restriction on generator output.The accuracy and fast solution ability of the proposed method are verified by applying it to a simple 3‐machine model and IEEJ EAST10‐machine standard model. Furthermore, the usefulness of the method for severity ranking of transient stability for various fault cases is discussed by using CGM. 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 173(3): 10–19, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ eej.21035
Prussian Blue‐Modified Titanate Nanotubes: A Novel Nanostructured Catalyst for Electrochemical Reduction of Hydrogen Peroxide
Authors: Iveković Damir , Gajović Andreja , Čeh Miran , Pihlar Boris 
Published: July 2010

AbstractPrussian blue (PB) modified titanate nanotubes (PB‐TiNT) have been synthesized by the reaction of Fe2+‐modified TiNT with hexacyanoferrate(III) ions. The rate constant for heterogeneous catalytic reaction between PB‐TiNT and H2O2 was found to be k=2×104 dm3 mol−1 s−1, which is an order of magnitude higher than the values of k reported for conventionally prepared, electrochemically deposited PB films. On the PB‐TiNT modified electrode with subnanomolar surface concentration of PB (Γ(PB)=2.8×10−11 mol/cm2), a stable, reproducible and linear response towards H2O2 was obtained in the concentration range 0.02–4 mM, with the sensitivity of 0.10 AM−1 cm−2 at −150 mV.
Poly(pyridine‐3‐boronic acid)/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrodes for Simultaneous Determination of Ascorbic Acid, 3,4‐Dihydroxyphenylacetic Acid and Uric Acid
Authors: Wu Zhijiao , Zhao Hong , Xue Ying , He Yujian , Li Xiangjun , Yuan Zhuobin 
Published: July 2010

AbstractPoly(pyridine‐3‐boronic acid) (PPBA)/multiwalled carbon nanotubes (MWCNTs) composite modified glassy carbon electrode (GCE) was used for the simultaneous determination of ascorbic acid (AA), 3,4‐dihydroxyphenylacetic acid (DOPAC) and uric acid (UA). The anodic peaks for AA, DOPAC and UA at the PPBA/MWCNTs/GCE were well resolved in phosphate buffer solution (pH 7.4). The electrooxidation of AA, DOPAC and UA in the mixture solution was investigated. The peak currents increase with their concentrations increasing. The detection limits (S/N=3) of AA, DOPAC and UA are 5 µM, 3 µM and 0.6 µM, respectively.
Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films
Authors: Wang Zhijie , Etienne Mathieu , Kohring Gert W. , Walcarius Alain 
Published: July 2010

AbstractThe influence of polyelectrolyte (PE) additives on the sol‐gel encapsulation of dehydrogenases has been evaluated using D‐sorbitol dehydrogenase (DSDH) as a model enzyme. Thin sol‐gel films were prepared on glassy carbon electrodes (GCE), which were then evaluated with respect to the electrochemical detection of D ‐sorbitol. DSDH encapsulation in pure silica thin films resulted in undetectable electrochemical signal. Adding positively‐charged PE that contributes to stabilize the negatively‐charged DSDH in the biocomposite film led to much faster and higher response of DSDH to its substrate, as shown by the significant improvement in bioelectrochemical detection of NADH produced during the oxidation of D‐sorbitol.
Quantitative Structure–Electrochemistry Relationship of 1‐Phenyl‐5‐benzyl‐sulfanyltetrazoles and Their Electrooxidation as a Metabolic Model
Authors: Nesměrák Karel , Doležal Rafael , Hudská Věra , Bártl Josef , Štícha Martin , Waisser Karel 
Published: July 2010

AbstractThe quantitative structure‐electrochemistry relationships study of nineteen derivatives of 1‐phenyl‐5‐benzylsulfanyltetrazole, that seem to be very promising antimycobacterial drugs, resulted in a good correlation relationship between half‐wave potential and energy of HOMO orbital. The study of electrochemical oxidation of these compounds as a model of their possible metabolic degradation was performed. It was possible to propose a scheme of their electrochemical oxidation: the electrochemical oxidation starts on the methoxy group, where by elimination of one electron the radical cation with very short life‐time is formed. The free electron is translocated to the position 2, followed by closing of new cycle with CH2 of benzylsulfanyl moiety. In the case of 2‐methoxyphenyl tetrazoles this process is followed by the desintegration of the tetrazole ring.
Glucose Biosensor Based on Silicon Nitride Nanoparticles
Authors: Salimi Abdollah , Zand‐Karimi Roaya , Noorbakhash Abdollah , Soltanian Saied 
Published: July 2010

AbstractFor the first time silicon nitride (Si3N4) nanoparticles was used for preparation electrochemical biosensor. GOx immobilized on the Si3N4 nanoparticles exhibits facile and direct electrochemistry. The surface coverage and heterogeneous electron transfer rate constant (ks) of immobilized GOx were 6.3×10−13 mol cm−2 and 47.4±0.3 s−1. The sensitivity, linear concentration range and detection limit of the biosensor for glucose detection were 38.57 µA mM−1 cm−2, 25 µM to 8 mM and 6.5 µM, respectively. This biosensor also exhibits good stability, reproducibility and long life time. These indicate Si3N4 nanoparticles is good candidate material for construction of third generation biosensor and bioelectronics devices.
Biparametric Potentiometric Analytical Microsystem Based on the Green Tape Technology
Authors: Ibáñez‐García Nuria , Baeza Mireia , Puyol Mar , Gómez Raquel , Batlle Montse , Alonso‐Chamarro Julian 
Published: July 2010

AbstractThe construction and evaluation of a Low Temperature Co‐fired Ceramics (LTCC)‐based microsystem prototype as a possible on‐site microanalyzer to monitor the presence of two ions in water simultaneously is presented. The approach has been assayed to detect nitrate and chloride ions as model analytes by means of integrating in the same single substrate an ion selective polymeric membrane to nitrate and two screen‐printed Ag/AgCl electrodes. One supplies the chloride ion concentration and the other is to complete the potentiometric detection system as the reference electrode. The results obtained by the full characterization of the microanalyzer prove the relevance of the proposal and the possibility to be transferred to real‐world samples and environmental monitoring.
A Diverse‐Stimuli Responsive Chemiluminescent Probe with Luminol Scaffold and Its Electropolymerization
Authors: Asil Demet , Cihaner Atilla , Algı Fatih , Önal Ahmet M. 
Published: July 2010

AbstractA diverse‐stimuli responsive chemiluminescent system, SNS‐Lum, and its electropolymerization to give PSNS‐Lum, which is the first example of conjugated polymers with pendant luminol arms inducing chemiluminescence in the presence of super oxide radical anion under neutral conditions, are highlighted.
Rapid Automatic Determination of Calcium and Magnesium in Aqueous Solutions by FIA Using Potentiometric Detection
Authors: Vahl Katja , Kahlert Heike , Scholz Fritz 
Published: July 2010

AbstractA pH‐sensitive detector for flow‐through potentiometry based on a graphite/quinhydrone/silicone composite electrode was applied to determine calcium and magnesium in aqueous solutions. The determinations are performed by FIA titrations with EDTA as the carrier solution. The method was applied to the analysis of mineral water, drinking water, and river water. The results were compared with those obtained by batch complexometric titration. The proposed new method is suitable for the simple, rapid and automated determination of water hardness in small sample volumes of aqueous solutions. The detection limit for both ions is about 1×10−5 mol L−1, the standard deviation is less than 2%. The sampling frequency is 60 determinations per hour.
Direct Determination of Copper in Biodiesel Using Stripping Analysis
Authors: Almeida Eduardo S. , Monteiro Marina A. N. A. , Montes Rodrigo H. O. , Mosquetta Rafael , Coelho Nívia M. M. , Richter Eduardo M. , Muñoz Rodrigo A. A. 
Published: July 2010

AbstractA simple, effective, and low‐cost protocol for copper determination in biodiesel, with no sample decomposition, is reported. Samples were diluted in an ethanol‐water solution (with HCl as supporting electrolyte) generating a homogeneous mixture at which copper was directly detected using stripping chronopotentiometry using a gold working‐electrode. The optimized mixture was 100 µL (0.088 g) of biodiesel, 15 mL of ethanol, and 5 mL of 0.1 mol L−1 HCl aqueous solution. The estimated detection limit was 200 ng g−1 (300‐s deposition time). The elimination of the sample treatment step offers the possibility of on‐site measurements in association with commercially‐available portable potentiostats.
Adsorptive Stripping Determination of Scandium(III) with Mordant Blue 9 on Silver Amalgam Film Electrode
Authors: Piech Robert 
Published: July 2010

AbstractA new adsorptive stripping voltammetric method for the determination of trace scandium(III) based on the adsorption of scandium(III)‐mordant blue 9 complex on the cyclic renewable mercury film silver based electrode (Hg(Ag)FE) is presented. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition are optimized. The calibration graph is linear from 2 nM (0.09 μg L−1) to 90 nM (4 μg L−1) for a preconcentration time of 45 s, with correlation coefficient of 0.9995. For a Hg(Ag)FE with a surface area of 7.9 mm2 the detection limit for a preconcentration time of 90 s is as low as 5 ng L−1. The repeatability of the method at a concentration level of the analyte as low as 0.2 μg L−1, expressed as RSD is 1.9 % (n=5). The proposed method was successfully applied and validated by studying the certified reference material (CRM 320 – river sediment) and natural samples with simultaneous recovery of Sc(III) from spiked water and sediment samples.
Captopril Electrochemical Oxidation on Fluorine‐Doped SnO2 Electrodes and Their Determination in Pharmaceutical Preparations
Authors: Armijo Francisco , Torres Ignacio , Tapia Ricardo , Molero Leonard , Antilén Mónica , del Río Rodrigo , del Valle Mª Angélica , Ramírez Galo 
Published: July 2010

AbstractA novel application of fluorine‐doped tin oxide (FTO) electrodes is reported in the present work. To this end, the captopril electrochemical oxidation mechanism on FTO electrodes at various pH and its determination in pharmaceutical preparations was investigated. Captopril oxidation on FTO proceeds at pH between 2.0 and 4.0. The study revealed that interferences for captopril determination in pharmaceutical samples was totally suppressed using these electrode materials. Voltammetric survey showed an anodic peak at about 0.375 V (Ag|AgCl) for captopril oxidation, that takes place through an EC process at pH interval 2.0–4.0. The investigation demonstrated that captopril oxidation occurs through protonated species and these electroactive species interact by adsorption on FTO electrodes, with a large heterogeneous rate constant and a mechanism involving 1H+/1e− in the global reaction. Moreover, a captopril sensor based upon FTO electrodes, with a linear range miliMolar, is proposed. These electrodes are promising candidates for the efficient electrochemical determination of captopril in pharmaceutical preparations.
Contrast‐enhanced 18F‐FDG‐PET/CT for the assessment of necrotic lymph node metastases
Authors: Haerle Stephan K. , Strobel Klaus , Ahmad Nader , Soltermann Alex , Schmid Daniel T. , Stoeckli Sandro J. 
Published: July 2010

AbstractBackground.Cystic lymph node metastasis (CLNM) is commonly found in human papillomavirus (HPV)‐associated tonsillar squamous cell carcinoma (SCC). The aim of this study was to compare the accuracy in detecting cystic lymph node metastasis from tonsillar SCC between contrast‐enhanced CT, 18F‐ fluorodeoxyglucose‐positron emission tomography (FDG‐PET), non‐enhanced 18F‐FDG‐PET/CT, and contrast‐enhanced 18F‐FDG‐PET/CT.Methods.Thirty‐four patients with a tonsillar SCC undergoing a pretreatment contrast‐enhanced 18F‐FDG‐PET/CT followed by a neck dissection as a standard of reference were included. The contrast‐enhanced CT part, the 18F‐FDG‐PET part, the non‐enhanced 18F‐FDG‐PET/CT part, and the contrast‐enhanced 18F‐FDG‐PET/CT were assessed separately for correct N classification and the differentiation of N0 versus N+.Results.Contrast‐enhanced 18F‐FDG‐PET/CT, non‐enhanced 18F‐FDG‐PET/CT, and contrast‐enhanced CT are equally accurate for correct neck staging. Regarding pN0 versus pN+, contrast‐enhanced CT and contrast‐enhanced 18F‐FDG‐PET/CT are superior to non‐enhanced 18F‐FDG‐PET/CT (p = .017).Conclusion.Contrast‐enhanced CT and contrast‐enhanced 18F‐FDG‐PET/CT perform equally and better than non‐enhanced 18F‐FDG‐PET/CT in detecting CLNM in tonsillar SCC. Therefore, in patients scheduled for 18F‐FDG‐PET/CT, we strongly suggest performing a contrast‐enhanced 18F‐FDG‐PET/CT, which is not routine in most centers. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Insulin‐like growth factor II mRNA‐binding protein 3: A novel prognostic biomarker for oral squamous cell carcinoma
Authors: Li Shengjin , Cha JeongDan , Kim Jin , Kim Ki‐Yeol , Kim Hyung‐Jun , Nam Woong , Cha In‐Ho 
Published: July 2010

AbstractBackground.Oral squamous cell carcinoma (OSCC) is caused by multiple factors, including carcinogen exposure. Insulin‐like growth factor II mRNA‐binding protein 3 (IMP3) is highly expressed in various cancer cells but is rarely expressed in normal cells. We investigated whether IMP3 can be used as a prognostic biomarker for OSCC.Methods.We performed immunohistochemistry and Western blotting to examine IMP3 expression in human tissues. We also investigated correlations among IMP3 expression, clinicopathologic factors, and overall survival.Results.IMP3 was overexpressed in OSCC cells. The expression was correlated with high histologic grade, lymph node metastasis, and advanced tumor and clinical stages. Univariate analysis indicated that advanced clinical stages, lymph node metastases, and IMP3 expression were predictive factors for OSCC. Multivariate analysis showed that IMP3 expression was an independent prognostic indicator for OSCC.Conclusions.IMP3 expression was related to various clinicopathologic factors. IMP3 expression was an independent prognostic factor in patients with OSCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Paratracheal lymph node dissection in cancer of the larynx, hypopharynx, and cervical esophagus: The need for guidelines
Authors: de Bree Remco , Leemans C. René , Silver Carl E. , Robbins K. Thomas , Rodrigo Juan P. , Rinaldo Alessandra , Takes Robert P. , Shaha Ashok R. , Medina Jesus E. , Suárez Carlos , Ferlito Alfio 
Published: July 2010

AbstractIn laryngeal, hypopharyngeal, and cervical esophageal carcinomas, the paratracheal lymph nodes (PTLN) may be at risk for lymph node metastasis. The presence of PTLN metastasis is an important prognostic factor for the development of mediastinal and distant metastases, stomal recurrence, and disease‐free and overall survival. Studies on PTLN metastasis are scarce. In most studies, PTLN dissection has not been routinely performed, and selection criteria for PTLN dissection are usually not well defined. Therefore, in most reported studies, selection bias is present and results are difficult to compare. The reported prevalence of PTLN metastases varies according to the site and stage of the primary tumor: subglottic cancer, transglottic cancer, and glottic cancer with subglottic extension have a higher risk of PTLN metastases. Diagnostic imaging is not sufficiently reliable to detect occult PTLN metastases and avoid unnecessary PTLN dissections. PTLN dissection is associated with limited morbidity, but damage to major vessels may occur, and because of exposure of these vessels PTLN may increase the morbidity of fistulae that can occur after total laryngectomy. The dissection may produce hypocalcemia, if performed bilaterally. Nevertheless, the limited morbidity and high rate of metastasis in specific laryngeal, hypopharyngeal, and cervical esophageal carcinomas argue in favor of routine elective PTLN treatment for these tumors. Large prospective studies are needed to identify the patients at risk with primary tumors in more detail. Moreover, improved diagnostic imaging is needed to detect (occult) PTLN metastases more reliably. Based on future studies, clinical guidelines have to be developed to avoid undertreatment and overtreatment. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Insulin‐like growth factor‐1 receptor inhibitor, AMG‐479, in cetuximab‐refractory head and neck squamous cell carcinoma
Authors: Chung Christine H. , Pohlmann Paula R. , Rothenberg Mace L. , Burkey Brian B. , Parker Joel , Palka Kevin , Aulino Joseph , Puzanov Igor , Murphy Barbara 
Published: July 2010

AbstractBackgroundRecurrent head and neck squamous cell carcinoma (HNSCC) remains a difficult cancer to treat. Here, we describe a patient with HNSCC who had complete response to methotrexate (MTX) after progressing on multiple cytotoxic agents, cetuximab, and AMG‐479 (monoclonal antibody against insulin‐like growth factor‐1 receptor [IGF‐1R]).MethodsThe clinical information was collected by a retrospective medical record review under an Institutional Review Board–approved protocol. From 4 tumors and 2 normal mucosal epithelia, global gene expression, and IGF‐1R and dihydrofolate reductase (DHFR) protein levels were determined.ResultsEffective target inhibition in the tumor was confirmed by the decreased protein levels of total and phospho‐IGF‐1R after treatment with AMG‐479. Decreased level of DHFR and conversion of a gene expression profile associated with cetuximab‐resistance to cetuximab‐sensitivity were also observed.ConclusionThis suggests that the combination of AMG‐479 and MTX or cetuximab may be a promising therapeutic approach in refractory HNSCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
An unusual cause of chest pain: Mycobacterium avium complex and the immune reconstitution inflammatory syndrome
Authors: Lin Richard J. , Song Jie 
Published: July 2010

AbstractThe HIV‐associated immune reconstitution inflammatory syndrome usually manifests as new infections or worsening of pre‐existing infections during the first few months of initiating anti‐retroviral therapy. It is commonly associated with local or systemic inflammation, presumably due to rapid reconstitution of host immune system. Here we describe a unique case of the immune reconstitution inflammatory syndrome presenting as acute pericarditis and pericardial effusion caused by mycobacterium avium complex. We also demonstrate that judicious use of steroids, along with pathogen specific antimicrobial therapy, can prevent local complications of the inflammatory response. Journal of Hospital Medicine 2010. © 2010 Society of Hospital Medicine.
Amino acids binding to Zn2+‐cyclen molecular receptor in aqueous solution
Authors: Vargová Z. , Balentová E. , Walko M. , Arabuli L. , Hermann P. , Lukeš I. 
Published: July 2010

AbstractPotentiometric titrations and1H NMR spectroscopic studies of amino acids binding to the [ZnL]2+‐complex where L = cyclen in aqueous solution provide information concerning complexing species identity, their stability, and coordination mode declaration. The amino acids form stable ternary [ZnL(HLn)]2+and [ZnL(Ln)]+complexes. The observations indicate bidentate coordination mode of the deprotonated amino acids, involving both the amine and the carboxylate functions to the [ZnL]2+complex in pH region of about 7.5–9.5. The determined stability constants indicate that [ZnL]2+complex is a very efficient receptor for simple amino acids such as glycine and alanine. Copyright © 2010 John Wiley & Sons, Ltd.