bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility study for the use of gene electrotransfer and cell electrofusion as a single-step technique for the generation of activated cancer cell vaccines
Authors: Ušaj Marko , Pavlin Mojca , Kandušer Maša 
Published: April 2024

Abstract

In the search for safe induction of cellular and humoral cancer immunity dendritic cell-based therapies hold great potential. This approach is based on manipulation of dendritic cells to activate immune system against specific cancer antigens. For the development of an effective cell vaccine platform, gene transfer, and cell fusion have been used for modification of dendritic or tumor cells to express immune (co)stimulatory signals and to load dendritic cells with tumor antigens. Both, gene transfer and cell fusion can be achieved by single technique, a cell membrane electroporation. The cell membrane exposed to external electric field becomes temporarily permeable, enabling introduction of genetic material, and also fusogenic, enabling the fusion of cells in the close contact. We tested the feasibility of combining gene electrotransfer and electrofusion into a single-step technique. We evaluated the effects of electroporation buffer, pulse parameters, and cell membrane fluidity on the efficacy of the combined method. We determined the percentage of fused cells expressing green fluorescence protein GFP in a murine cell model of melanoma B16F1, an often-used cell line in pre-clinical studies. Our results suggest that gene electrotransfer and cell electrofusion can be applied in a single procedure. The percentage of viable hybrid cells expressing GFP depends on electric pulse parameters and the composition of the electroporation buffer. Cell membrane fluidity cannot be related to the efficiency of this technique. Further optimization of electric pulse parameters and buffers has to be accomplished before this technique can be used for preparation of effective dendritic cell-based vaccines.
Unraveling the Role of Water in Mechanism Changes for Economically Viable Catalytic Plastic Upcycling
Authors: Ro Insoo , Kwon Taeeun , Ahn Byeongchan , Kang Ki Hyuk , Won Wangyun 
Published: April 2024

Abstract

The surge in global plastic production, reaching to 390.7 million tons in 2021, exacerbates environmental pollution, with only 11% of plastic being recycled. Catalytic recycling, particularly through hydrogenolysis and hydrocracking, offers a promising avenue for upcycling polyolefin plastic, comprising 55% of global plastic waste. This study investigates the influence of water on polyolefin depolymerization using Ru catalysts, revealing a promotional effect only when both metal and acid sites, particularly Brønsted acid, are present. Findings highlight the impact of Ru content, metal-acid balance, and their proximity on this interaction, as well as their role in modulating the isomerization process, affecting product selectivity. Additionally, the interaction facilitates the suppression of coke formation, ultimately enhancing catalyst stability. A comprehensive techno-economic and life cycle assessment underscores the viability and environmental benefits of the process, particularly in the presence of water. These insights advance understanding and offer strategies for optimizing polyolefin plastic recycling processes.
The Effect of Whitening Toothpastes on the Surface Properties and Color Stability of Different Ceramic Materials
Authors: Geduk Şükriye Ece , Sağlam Gaye 
Published: April 2024

Abstract

Objectives
This study aimed to evaluate the effect of whitening toothpastes on the surface roughness and colour change of CAD-CAM materials.
Materials and methods
A total of 96 samples (2x10x12 mm3) were prepared from Cerasmart (CS) and Celtra Duo blocks. Celtra Duo samples were divided into two groups. One group was fired with glaze paste (CDC) and the other was not treated (CD). All groups were then divided into 4 subgroups (n = 8). The groups were brushed with conventional (Colgate™ Max Fresh), silica (Opalescence™), charcoal (Curaprox™ Black in White) and blue covarin (Signal™ White Now) toothpastes for 30,000 brushing cycles. The initial and final surface roughness values were measured with contact profilometer and a dental spectrophotometer used for obtaining colour values. One sample from each brushed group analyzed using a surface electron microscope. Data analyzed with Kruskal-Wallis and Mann-Whitney tests. (p = 0.05)
Results
The surface roughness of CS samples brushed with Opalescence™ and Curaprox™ was significantly higher than CD and CDC. Surface roughness change values of CS samples brushed with Curaprox™ were significantly higher than the CD and CDC. Curaprox™ brushed samples showed significant difference in colour change values for all materials.
Conclusions
Brushing increases the surface roughness of CAD-CAM ceramic materials. The roughness of resin-based materials is higher than zirconia-reinforced lithium silicates. Silica-contained toothpastes may cause discoloration of nanoceramic and zirconia reinforced glass ceramic restorations.
Clinical relevance
It should be clinically considered that whitening toothpastes may cause roughness in ceramic materials and change the desired color.
Predicting Suicide Among US Veterans Using Natural Language Processing-enriched Social and Behavioral Determinants of Health
Authors: Mitra Avijit , Chen Kun , Liu Weisong , Kessler Ronald C. , Yu Hong 
Published: April 2024

Abstract
Despite recognizing the critical association between social and behavioral determinants of health (SBDH) and suicide risk, SBDHs from unstructured electronic health record (EHR) notes for suicide predictive modeling remain underutilized. This study investigates the impact of SBDH, identified from both structured and unstructured data utilizing a natural language processing (NLP) system, on suicide prediction within 7, 30, 90, and 180 days of discharge. Using EHR data of 2,987,006 Veterans between October 1, 2009, and September 30, 2015, from the US Veterans Health Administration (VHA), we designed a case-control study that demonstrates that incorporating structured and NLP-extracted SBDH significantly enhances the performance of three architecturally distinct suicide predictive models - elastic-net logistic regression, random forest (RF), and multilayer perceptron. For example, RF achieved notable improvements in suicide prediction within 180 days of discharge, with an increase in the area under the receiver operating characteristic curve from 83.57–84.25% (95% CI = 0.63%-0.98%, p-val < 0.001) and the area under the precision recall curve from 57.38–59.87% (95% CI = 3.86%-4.82%, p-val < 0.001) after integrating NLP-extracted SBDH. These findings underscore the potential of NLP-extracted SBDH in enhancing suicide prediction across various prediction timeframes, offering valuable insights for healthcare practitioners and policymakers.
Agricultural Irrigation Water Requirement and Its Response to Climatic Factors Based on Remote Sensing and Single Crop Coefficient Method
Authors: Sun Jiaxin , Chen Liwen , Qi Peng , Zhang Guangxin 
Published: April 2024

Abstract

Precise calculation of the water required for agricultural irrigation is important for efficient use of water resources and maintenance of food security. However, the amount of water required for agricultural irrigation is significantly uncertain at different time scales under the influence of climate change. In this study, one of major grain producing area, Youyi Farm in the Sanjiang Plain, was selected to simulate the agricultural irrigation water requirement for the Rice, Maize, and Soybean based on remote sensing data on the Google Earth Engine (GEE) platform sensing and single crop coefficient method. Meanwhile, their response to climatic factors was analyzed by the method of generalized additive model (GAM). The results showed that the multi-year average irrigation water requirement of Rice, Maize, and Soybean during 2010 ~ 2019 was 2.98×108m3, 0.25×108m3, and 0.04×108m3, respectively. A fluctuating decreasing trend was shown for each crops’ water requirement. The requirement for irrigation water at different stages of crop growth varies significantly due to different climatic conditions in different years. For Rice, the initial growth period accounts for the largest proportion of irrigation water requirement (34%). For Maize and Soybean, the middle growth period has the highest proportion of irrigation water requirement (45% and 52%). In addition, from 2000 to 2019, the three main climatic factors with the greatest impact on irrigation water requirement were precipitation, vapor pressure deficit, and photoperiod, in that order. Irrigation water requirement was positively correlated with wind speed, photoperiod, maximum temperature and vapor pressure deficit. Precipitation is negatively correlated with irrigation water requirement, and minimum temperature is non-linearly correlated with irrigation water requirement, first positively and then negatively.
Bio efficacy of calotropis procera leaf extract against Aedes vittatus (Ditera:culicidae)
Authors: ANJAIAH CHANDRA 
Published: April 2024

Abstract
Background:
The present study was conducted to know the larvicidal effects of native plant, Calotropis procera (Giant milk weed). Leaves and flowers of the plant were used to check the larvicidal activity.
Methods:
Microwave assisted extraction was used for material extraction. Different concentrations (20%, 40%, 60%, 80% and 100%) of the leaf and flower extract were used. Mortality of the larvae was observed after 24hrs.
Results:
Results showed the effectively of higher concentrations was greater as compared to lower concentrations. Leaf extract was found to be more significant in killing the larvae. Results showed that 100% and 75% mortality of larvae. Was observed in beakers containing 100 ml concentration leaf and flower extract respectively.
Conclusions:
It is concluded that all parts of this plant can be used for the prevention and control of Ae. vittatus mosquitoes due to its pesticide properties and bioactive chemicals.
Phytochemical composition, antioxidant and cytotoxicity of the aqueous extracts of Dracaena arborea andBridelia ferruginea: in vitro and in silico studies.
Authors: Deeh Patrick Brice Defo , Kim Moonhae , Sathiyaseelan Anbazhagan , Naveen Kumar Vishven , Wang Myeong-Hyeon 
Published: April 2024

Abstract
This study was undertaken to determine the phytochemical profile, antioxidant and cytotoxicity of the aqueous extracts of Dracaena arborea (DA) and Bridelia ferruginea (BF). The phytochemical composition, total phenolic (TP) and flavonoid (TF) contents of the extracts were determined by GC-MS, Folin Ciocalteu and AlCl3 methods, respectively. The antioxidant power was estimated using DPPH and ABTS+ radicals scavenging method, and cupric and ferric reducing capacity assay. The effect of extracts on hemolysis was also determined using red blood cells. Selected phytocompounds were docked against some oxidative stress (Keap1 and GST) and cytotoxicity (PARP10 and p90 RSK) proteins. The TP and TF content of BF was significantly (p < 0.001) higher than that of DA. The DPPH, ABTS+, cupric and ferric reducing activity of BF were significantly (p < 0.05 − 0.001) higher than those of DA. DA decreased the viability of PC3, NIH3T3 and BT474 cells in a dose-dependent manner, while BF tended to feed the cells. Drug-Likeness and toxicity prediction of selected compounds found in the plant extracts were within the acceptable standards, according to Lipinski’s rule. BF has the possibility to be exploited in the prevention/treatment of oxidative stress-related diseases, while DA could be a potential anticancer agent.
Determination of the state of artificial plantings of the green belt of Astana using remote sensing data of the earth
Authors: Кабанов А.Н. , Бекбаева А.М. , Кабанова С.А. , Кочегаров И.С. , Данченко М.А. , Скотт С.А. 
Published: April 2024

Для определения дальнейшей стратегии сохранения и содержания искусственных насаждений зеленого пояса г. Астаны было необходимо оценить жизненное состояние древесных и кустарниковых растений, что стало первоочередной задачей наших исследований. Для этого проводились наземные таксационные наблюдения, использовались мультиспектральные данные дистанционного зондирования Земли (ДЗЗ) и инструменты GIS. Целью исследований являлось определение площадей ослабленных и погибающих лесных культур в зеленом поясе г. Астаны с помощью данных ДЗЗ. Методика исследований заключалась в закладке эталонных участков в лесных культурах различного породного состава и возраста, в которых проводилось таксация деревьев и определение их жизненного состояния. На основе дешифрирования мультиспектральных снимков эталонных участков производилась идентификация породного состава и жизненного состояния искусственных насаждений всей зеленой зоны г. Астаны. В результате проведения наземных исследований и обработки данных ДЗЗ выявлено, что в зеленом поясе г. Астаны основную площадь занимают лесные культуры, относящиеся к жизненному состоянию «ослабленные» - 40,3%. «Здоровые» лесные культуры занимают 31,4% площади, «погибающие» - 28,3%.
To determine the further strategy of conservation and maintenance of artificial plantings of the green belt of Astana, it was necessary to assess the vital condition of woody and shrubby plants, which became the primary task of our research. For this purpose, ground-based taxational observations were carried out, multispectral data of remote sensing of the Earth (remote sensing) and GIS tools were used. The purpose of the research was to determine the areas of weakened and dying forest crops in the green belt of Astana using remote sensing data. The research methodology consisted in laying reference plots in forest crops of various species composition and age, in which trees were taxed and their vital condition was determined. Based on the decoding of multispectral images of reference sites, the identification of the rock composition and the living condition of artificial plantings of the entire green zone of Astana was carried out. As a result of ground-based research and remote sensing data processing, it was revealed that in the green belt of Astana, the main area is occupied by forest crops related to the «weakened» state of life - 40.3%. «Healthy» forest crops occupy 31.5% of the area, «dying» - 28.3%.
Using disability-adjusted life years measure for characterization of radiation risk from fluoroscopy
Authors: Repin L. V. , Akhmatdinov R. R. , Biblin A. M. , Vodovatov A. V. , Shatskiy I. G. 
Published: April 2024

Medical diagnostics procedures involving the use of ionising radiation is one of the most intensively developing areas in medical diagnostics. At the same time, medical exposure of patients has a number of fundamental differences from other routine situations of planned exposure. As a rule, medical exposure is acute, and radiation doses in some types of examinations exceed the level of natural and man-made exposure associated with normal operation of radiation facilities. In such a situation, risk differentiation is of great importance, taking into account the different radiosensitivity of individual sex and age groups of patients. Radiation risks should be taken into account both when prescribing examinations involving the use of ionising radiation and when analysing the benefit-harm ratio associated with their application to specific patients. Another reason why risk assessment is an urgent task is the need to inform patients and/or their legal representatives about the health risks associated with the carrying out of examinations, including radiation risks. Within the framework of the present work, the risks associated with fluoroscopic examinations were assessed. To characterise the risk we used the DALY indicator, which is more and more frequently used in risk assessment, reflecting the possible number of lost years of healthy life as a result of radiation exposure during the study. The aim of the work was to calculate DALY values for different sex and age groups of patients from the Russian population during fluoroscopic examinations. To achieve this aim, the method of estimating the number of healthy life years lost due to exposure to ionising radiation, which was developed in the course of the research work, was used. The paper presents the results of calculating DALY values for three types of fluoroscopic examinations. This work represents the next step in the direction of harmonisation of radiation risk assessment methodology with the methodologies of risk assessment of other nature. One of the directions of using the calculation results is the development of methodological approaches to the calculation of sex- and age-dependent risk coefficients associated with medical exposure of patients. The analysis of the obtained results showed that the contribution of oncological diseases to the total radiation harm in older men was significantly higher than in women.
The News Media in Raising Conflicts Tension: A Critical Study Toward News Conflict Between Israel and Palestine
Authors: AR M Fikri 
Published: April 2024

<p class="Default"><em><span lang="TR">This study analyzes the news about conflict between Israel and Palestine in online news media Foxnews.com on May 2021. The characteristics of news from these news media have quite specific characteristics, which is a strong tone, using open visualization, inviting debate, and succeeding in getting thousands of comments from the audience. In this point, even though the news is read by many people, it is also has the potential to exacerbate conflict in the field. This study adopts Teun A. van Dijk’s Critical Discourse Analysis method which develops analysis from the microstructure, superstructure, and macrostructure levels. The micro structure level looks at the local meaning of the news text from the choice of diction, sentence structure, and rhetoric. Then, the super structure level examines the news scheme, consisting of introduction, content and conclusion. Furthermore, macro structural analysis tries to see the global meaning of news texts by looking at news topics and the surrounding social context. The results of the study show that the Israel and Palestine conflict is not only due to the conflict of one of the group carrying out the attack. But at the same time it was also caused by hard conflict news, there was trauma to the victims in the conflict, as well as the global security conditions still not stable until now. </span></em><em><span lang="EN-US"> </span></em></p>
Effects of Injury Registry Data on Policy Making, Hospitalizations, and Mortality: Protocol for a Systematic Review and Meta-Analysis
Authors: Medeiros-de-Souza Ana Cláudia , Lopes Luana Emanuelly Sinhori , de Oliveira Bruno Zocca , Rother Edna Terezinha , Correia Lucas Reis 
Published: April 2024

Background
Initiated in 2021, a Brazilian project aims to establish a national injury registry, compiling comprehensive data on events and individuals across the country, irrespective of injury severity. The registry integrates information from prehospital and hospital care, diverse health systems lacking interoperability, and sectors such as firefighters and the police. Its primary goal is to enhance health surveillance by providing timely, high-quality information, guiding prevention strategies, and informing policy making. The project still aims to reduce long-term morbidity and mortality associated with injuries.

Objective
A knowledge gap remains regarding the effects of injury registries in relation to policies and injury outcomes. This protocol outlines a systematic review and meta-analysis to answer “What is the effect of implementation and use of injury registry data on policy making, hospitalization, and mortality?”

Methods
The systematic review follows PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, focusing on studies reporting results related to the implementation and use of injury registries, including trauma registries. Outcomes of interest include policy making, hospitalization rates or duration, and mortality. Registries within well-defined administrative boundaries will be included. Data will be collected from PubMed, Embase, Scopus, Web of Science, Lilacs, and references. Records will be independently screened by 2 reviewers, with any disagreements resolved through arbitration by a third reviewer. Homogeneous studies, with 3 or more evaluating the same outcome, may undergo meta-analysis. Subgroup analyses by registry type, injury groups, and other selected variables of interest will be conducted. Sensitivity analysis, risk of bias assessment, publication bias evaluation, and quality appraisal will also be performed.

Results
This systematic review will run from November 2023 to June 2024. No identical review was found. Search strategies were finalized, the bibliographic search started, duplicates were eliminated, and title and abstract screening began. Of 35 studies retrieved, 85 were excluded due to duplication, leaving 50 for selection.

Conclusions
This study is timely, aligning with ongoing national efforts to implement an injury registry. By synthesizing available evidence, we will identify the potential of injury registries to guide the decisions of Brazilian policy makers.

Trial Registration
PROSPERO CRD42023481528; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=481528

International Registered Report Identifier (IRRID)
PRR1-10.2196/55029
Cost Effectiveness General Anesthesia Combined with Scalp Block Compared to General Anesthesia in Patients Undergoing Removal Tumor Craniotomyin Dr. Sardjito Hospital
Authors: Wicaksono Galih Sahid , Sudadi  , Uyun Yusmein 
Published: April 2024

Background. Providers of anesthesia services have many opportunities to reduce these costs, of course with the aim of maintaining balance between profit, security and costs. Craniotomy surgery removal of the scalp block tumor is an alternative option that can be combined with general anesthesia. The scalp block itself can blunt stress response in craniotomy and can maintain unwanted hemodynamic instability.
Purpose. Find out the cost effectiveness of the scalp block in craniotomy patients removing tumors at Dr. SARDJITO Hospital.
Method. Method. Twenty patients ranging in age from 18 - 65 years with intracranial tumors who underwent tumor binding craniotomy at RSUP Dr. SARDJITO is grouped into two treatment groups with general anesthesia and general anesthesia with a combination of scalp blocks, both techniques are calculated using drugs and consumables during the operation.
Results. Total cost on the general anesthesia combined scalp block was Rp. 1,347,276 lower Rp. 377,833 compared to general anesthesia Rp. 1,725,109 with a significant difference (p = 0.005). Similarly, the hourly cost of the group in the combined general anesthesia scalp block was Rp. 286,351 Rp. 97,107 lower than general anesthesia Rp. 383,457 with a significant difference (p = 0.038).
Conclusion. Combination of general anesthesia and scalp block using 0.5% levobupivacaine more cost effective than general anesthesia and scalp block with placebo in supratentorial tumor patients undergoing tumor removal craniotomy.