bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring sources of knowledge utilized in practice among Jordanian registered nurses
Authors: Al‐Ghabeesh Suhair Husni , Abu‐Moghli Fathieh , Salsali Mahvash , Saleh Mohammad 
Published: May 2012

AbstractRationale, aims and objectives  Understanding sources of knowledge used in everyday practice is very helpful in improving the quality of health care services. There is a consensus in the literature that nurses mostly relied in their practice on experiential knowledge gained through their interactions with other members of health care professionals and patients. The general aim of this study is to explore the sources of knowledge Jordanian registered nurses use during their practice.Method  A descriptive correlational design was used to collect data from 539 Jordanian registered nurses from 10 hospitals using a self‐administered questionnaire.Results  The mean year of experience of the sample was 7.08 years. Of the 615 questionnaires distributed, 555 were returned. This yields a response rate of 87.6%. Results revealed that the top five ranked sources used by Jordanian registered nurses include: the information that nurses learned during nursing education, personal experience in nursing over time, what was learned through providing care to patients, information gained through discussion between physicians and nurses about patients, and information from policy and procedure manuals.Conclusion  Jordanian registered nurses recognize the value of research and that research utilization (RU) is an important issue and must not be ignored. The study has many implications for practice, education and research. Health care managers and decision makers need to play a more visible and instrumental role in encouraging RU to improve patients' quality of life.
Lymphedematous verrucous changes simulating squamous cell carcinoma in long‐standing hidradenitis suppurativa
Authors: Chu Emily Y. , Kovarik Carrie L. , Lee Robert A. 
Published: May 2012

AbstractBackground  Hidradenitis suppurativa (HS) is a relatively common condition marked by abscesses, sinus tracts, and scarring in intertriginous regions of the body. Lymphedema is an under‐recognized consequence of chronic inflammation in the setting HS.Methods  We report five cases of patients with lymphedema resulting from long‐standing HS; the patients were seen either in a specialty HS clinic or general dermatology clinic at our institution. Biopsies for histologic analysis were performed on four of the five patients reported here.Results  Changes of lymphedema may be manifested diffusely as widespread plaques or focally as verrucous papules and nodules. The latter may be difficult to distinguish clinically from squamous cell carcinoma, another complication of chronic HS. Histopathologic features of lymphedema include dilated lymphatic channels, diffuse edema, verrucous epidermal hyperplasia, mixed inflammation, and dermal fibrosis.Conclusion  Lymphedema associated with chronic HS, which we also term “verrucous lymphostasis,” encompasses a spectrum of clinical and histopathologic findings. Squamous cell carcinoma may mimic focal presentations of lymphedema, and biopsy may be necessary to distinguish the two.
Evaluating suitable internal control genes for transcriptional studies in heat‐stressed mammary explants of buffaloes
Authors: Sodhi M. , Kishore A. , Khate K. , Kapila N. , Mishra B.P. , Kataria R.S. , Mohanty A.K. , Varshney N. , Mukesh M. 
Published: May 2012

SummaryIt is now a well‐accepted notion that each new experimental design requires proper evaluation of internal control genes (ICGs) for accurate normalization of expression data. In riverine buffaloes, till date no appropriate ICG has been reported for studying transcriptional response under any of the physiological stressful condition. The objective here was to test 16 well‐known reference genes from different functional categories that could serve as suitable ICG during heat stress studies in buffalo mammary tissue. Briefly, the mammary explants were exposed to 45°C for 1 h and subsequently allowed to recover at 37°C for different time points (2–24 h). Three software programs, geNorm, Normfinder and BestKeeper, were used to measure gene transcript stability. RPL22 was excluded because of weak amplification and unacceptable PCR efficiency. Except GAPDH, all other genes showed expression stability within the acceptable range (<1.5). RPL4, B2M, RPS23 and EEF1A1 genes were found to be most stably expressed while GAPDH and ACTB showed least stability. The BestKeeper analysis identified high correlation for RPL4 (r = 0.953) and EEF1A1 (r = 0.914) with BestKeeper index. Based on the present findings, it could be suggested that geometric average of RPL4, B2M, RPS23 and EEF1A1 would provide accurate normalization to transcriptional data of buffalo mammary explant in response to heat stress.
Characteristics of F1 Hybrids and Inbred lines in Octoploid Strawberry (Fragaria × ananassa Duchesne)
Authors: Rho Il Rae , Woo Jong Gyu , Jeong Ho Jeong , Jeon Heung Yong , Lee Choon‐Hwan 
Published: May 2012

With 4 figures and 3 tablesAbstractThe strawberry (Fragaria × ananassa Duchesne) plant is an octoploid vegetative propagated plant, and its cultivation is labour intensive in comparison with other plants propagated from seeds. The aims of this study were to develop an inbred line from octoploid strawberry and to generate an F1 hybrid cultivar through the combination of inbred lines. All inbred lines were obtained through selfing using five cultivars. Inbred lines were produced by selecting the best individuals for further inbreeding from each of the previous generations. Plant vigour and yield in inbred lines were low compared with the original cultivar, but completely recovered in the F1 hybrid. Breeding of inbred lines in octoploid strawberry can be achieved if individuals showing inbreeding depression are eliminated and vigorous individuals are selected, resulting in a strong heterosis of F1 hybrids through crossing between inbred lines.
Molecular mapping of the vein colour locus in Solanum venturii
Authors: Park Tae‐Ho 
Published: May 2012

With 2 figuresAbstractThe genetic background of red or purple pigments derived from anthocyanin has not been firmly identified in potato, although the trait of tuber colour has been considered a breeding target owing to its visual appeal and valuable antioxidants in the human diet. In this study, we evaluated the genetic segregation of vein colour in the mapping population derived from a cross within a wild potato species, Solanum venturii. The segregation ratio was 1 : 1 for purple and green colours, indicating the gene for vein colour was monogenically inherited. The genetic position of the vein colour locus was expected to be on potato chromosome 4 with the bridged molecular markers, PATMATC_499 and RGH3. These results will facilitate the characterization and cloning of pigmentation‐related genes, and the colours of above‐ground tissues could be used as selection markers for accelerating the breeding of colour potatoes.
Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc‐finger nucleases
Authors: Kawai Narudo , Ochiai Hiroshi , Sakuma Tetsushi , Yamada Lixy , Sawada Hitoshi , Yamamoto Takashi , Sasakura Yasunori 
Published: May 2012

Zinc‐finger nucleases (ZFNs) are engineered nucleases that induce DNA double‐strand breaks (DSBs) at target sequences. They have been used as tools for generating targeted mutations in the genomes of multiple organisms in both animals and plants. The DSB induced by ZFNs is repaired by non‐homologous end joining (NHEJ) or by homologous recombination (HR) mechanisms. Non‐homologous end joining induces some errors because it is independent of a reference DNA sequence. Through the NHEJ mechanism, ZFNs generate insertional or deletional mutations at the target sequence. We examined the usability, specificity and toxicity of ZFNs in the basal chordate
Ciona intestinalis. As the target of ZFNs, we chose an
enhanced green fluorescent protein (EGFP)
gene artificially inserted in the
C. intestinalis genome because this locus is neutral for the development and growth of
C. intestinalis, and the efficiency of mutagenesis with ZFNs can thus be determined without any bias. We introduced
EGFP
‐ZFN mRNAs into the embryos of an
EGFP
‐transgenic line and observed the mutation frequency in the target site of
EGFP
. We also examined the effects of the
EGFP
‐ZFNs at off‐target sites resembling the
EGFP
target sequence in the
C. intestinalis genome in order to examine the specificity of ZFNs. We further investigated the influence of ZFNs on embryogenesis, and showed that adequate amounts of ZFNs, which do not disrupt embryogenesis, can efficiently induce mutations on the on‐target site with less effect on the off‐target sites. This suggests that target mutagenesis with ZFNs will be a powerful technique in
C. intestinalis.
Anatomy and ultrastructure of the floral nectary in Peganum harmala L. (Nitrariaceae)
Authors: ABEDINI MASUMEH , MOVAFEGHI ALI , ALIASGHARPOUR MAHBUBEH , DADPOUR MOHAMMAD REZA 
Published: May 2012

AbstractAnatomy and ultrastructure of the floral nectary of Peganum harmala L. were studied using light and transmission electron microscopy. The floral nectary was visible as a glabrous, regularly five‐lobed circular disc encircling the base of the ovary. Anatomically, it comprised a single layered epidermis and 15–20 layers of small, subepidermal secretory cells overlying several layers of large, ground parenchyma cells. The floral nectary was supplied by phloem and both sieve tubes and companion cells were found adjacent to the ground parenchyma. Based on our ultrastructural observations, plastids of secretory cells during the early stages of development were rich in starch grains and/or osmiophilic plastoglobuli, but these disappeared as nectar secretion progressed. The nectar appeared to exude through the modified stomata along symplastic and apoplastic routes. The abundant plastids and mitochondria suggest an eccrine mechanism of nectar secretion in P. harmala.
Pulmonary hemorrhage in very low‐birthweight infants: Risk factors and management
Authors: Chen Ying‐Yao , Wang Hsiao‐Ping , Lin Shu‐Ming , Chang Jenn‐Tzong , Hsieh Kai‐Sheng , Huang Fu‐Kuei , Chiou Yee‐Hsuan , Huang Yung‐Feng ,   Taiwan Premature Infant Development Collaborative Study Group
Published: May 2012

AbstractBackground:  Beginning 2007, the intratracheal route of epinephrine to end massive pulmonary hemorrhage (MPH) in very low‐birthweight (VLBW) infants was modified at Kaohsiung Veterans General Hospital. The aim of the present study was to assess the change in outcomes for these infants, and to evaluate the risk factors of MPH.Methods:  Using the database of the Premature Baby Foundation of Taiwan, the mortality, risk factors and characteristics of VLBW infants with or without MPH were compared between 2000–2006 and 2007–2010.Results:  Between January 2000 and December 2010, 399 VLBW infants were admitted to Kaohsiung Veterans General Hospital. Mean birthweight (BW) was 1099.6 ± 272.7 g, and mean gestational age (GA) was 28.7 ± 2.9 weeks. The overall survival rate was 84.2%. Sixteen (4%) had MPH: 11 in the first group (2000–2006; 18.2% survival rate), and five in the second group (2007–2010; 80% survival rate; P= 0.0000002). Infants with MPH had lower mean BW (864.9 ± 301.4 g, P= 0.0004), smaller mean GA (26.1 ± 2.0 weeks, P= 0.0002), significantly lower Apgar scores at 1 and 5 min, higher severity of respiratory distress syndrome (RDS; grades 3 or 4), and greater use of surfactant than infants without MPH. They also had more intraventricular hemorrhage and higher mortality.Conclusions:  Smaller GA, lower BW, lower Apgar scores at 1 and 5 min, severe RDS (grades 3 or 4), and use of surfactant place VLBW infants at greater risk of MPH. Proper prenatal care and preventing premature labor and delivery were the most important preventative factors. A quick, deep thrust of intratracheal epinephrine with a catheter may improve survival.
Grief reaction model of families who experienced acute bereavement in Japan
Authors: Tatsuno Junko , Yamase Hiroaki , Yamase Yoshie 
Published: May 2012

AbstractThe present study clarified the structure of factors that affect grief reactions of families who experienced acute bereavement in critical care settings in Japan. Sixty‐four families who experienced acute bereavement answered a questionnaire. The questionnaire included the Miyabayashi Grief Measurement, recognition of bereavement, Multidimensional Scale of Perceived Social Support, and the Tri‐Axial Coping Scale. We analyzed the causal structure regarding the relationship of stress recognition, coping, and grief reactions using structural equation modeling. The greatest influence on grief reactions of bereaved families was stress recognition. Factors that influenced stress recognition were subjective degree of sadness, acceptance of bereavement, regret for bereavement, and recognition of a peaceful death. These results show that the quality of end‐of‐life care in critical care settings is an important factor that affects bereaved families' stress recognition and grief reactions. Nurses and medical staff must provide end‐of‐life care to help family members accept the death of their loved one and reduce regrets as much as possible.
Development of a patient‐specific simulator for laparoscopic renal surgery
Authors: Makiyama Kazuhide , Nagasaka Manabu , Inuiya Toru , Takanami Kentaro , Ogata Masato , Kubota Yoshinobu 
Published: May 2012

Objectives:  To describe the development of a patient‐specific simulator for laparoscopic renal surgery.Methods:  Image data of each patient scheduled to undergo laparoscopic renal surgery are captured by the simulator, enabling each patient's organs to be reproduced. The surgeon can carry out a preoperative “rehearsal” of the operation by using a simulator based on patient‐specific data.Results:  The simulator is programmed to be adapted to both laparoscopic and retroperitoneoscopic surgery. The scope and the trocars can be located anywhere on the skin, and visualized on the monitor of the simulator. Dissection of the renal hilum can be simulated based in the anatomy of each patient. The haptic device of the simulator provides interactive resistance between the organs and surgical tools during the simulation.Conclusions:  This patient‐specific simulator has been developed with the purpose of providing surgeons with a practical training tool for laparoscopic renal surgery. Using specific data for each patient, the simulator enables surgeons to carry out a “rehearsal” operation.
Exam‐indicated cerclage in patients with fetal membranes at or beyond external os: A retrospective evaluation
Authors: Curti Alessandra , Simonazzi Giuliana , Farina Antonio , Mehmeti Hilda , Facchinetti Fabio , Rizzo Nicola 
Published: May 2012

AbstractAim:  To evaluate pregnancy outcome in women with fetal membranes at or beyond external os who underwent exam‐indicated cerclage or conservative management.Material and Methods:  Retrospective cohort study including 52 patients with fetal membranes at or beyond external os between 17 and 27 weeks of gestation, treated in two third‐level hospitals, between January 2001 and April 2009. The outcomes of interest of the study, prolongation of pregnancy and neonatal survival rate, were stratified according to type of management, parity, clinical conditions and blood tests at admission and calculated using the Kaplan–Meier algorithm.Results:  Of 52 women, 37 received exam‐indicated cerclage and 15 were managed conservatively. The rate of patients still pregnant beyond 180 days within the cerclage group differed significantly from those of the conservative management group (80% vs 0%, respectively) (P‐value < 0.001). No difference has been found according to neonatal survival (82% versus 53%, respectively).Conclusion:  Exam‐indicated cerclage appears to prolong gestation compared to expectant management.
Value of serum urocortin concentration in the prediction of preterm birth
Authors: Kashanian Maryam , Bahasadri Shohreh , Ghasemi Afsaneh , Bathaee Saeedesadat 
Published: May 2012

AbstractAim:  Preterm delivery is a serious problem during pregnancy with remarkable neonatal adverse effects. Prediction of preterm delivery in women with preterm uterine contractions or signs of preterm labor is critical because if these women are identified they can be referred to tertiary centers. The present study aimed to evaluate the value of maternal serum urocortin concentration for predicting preterm delivery in women with signs of spontaneous preterm labor.Material and Methods:  A cohort study was conducted on pregnant women at a gestational age of 28–36 weeks who were admitted to the labor ward with spontaneous preterm labor. A blood sample was obtained from all participants to measure serum urocortin. The women were monitored up to delivery and serum urocortin was compared between women with preterm delivery and those who delivered at term (37 weeks of gestation). Receiver Operating Characteristic (ROC) curve analysis was used to determine sensitivity and specificity if applicable.Results:  One hundred and sixty pregnant women finished the study. One hundred and forty‐eight (92.5%) of the women delivered preterm. Mean serum urocortin in the preterm delivery group was higher than in the term group, but without statistical significant difference (392.6 ± 29.23 vs 113.2 ± 11.0. pg/mL, respectively, P = 0.252). Area under the ROC curve was 0.6, which shows that this test is not appropriate for predicting preterm delivery in women with preterm labor.Conclusion:  Serum urocortin could not predict women who delivered preterm among women with signs of preterm labor.
Outcome of Prolift mesh repair in treatment of pelvic organ prolapse and its effect on lower urinary tract symptoms: 5‐year retrospective case study
Authors: Gad Nader , Duvvuru Amulya , Burchgart Brook 
Published: May 2012

AbstractAim:  To assess the outcome of the Prolift Gynecare (Ethicon, Somerville, NJ, USA) mesh repair in the treatment of pelvic organ prolapse. This includes the anatomical success, complication rate and effect on associated lower urinary tract symptoms.Material and Methods:  Retrospective case analysis of the above outcomes of 41 Prolift mesh repairs performed on 40 women over a 5‐year period by a single surgeon in a private hospital in Australia.Results:  Successful anatomical correction and bulge symptom resolution were observed in 100% (2/2), 91.6% (11/12) and 100% (27/27) of anterior, posterior and total Prolift, respectively. Prolapse in a non‐treated compartment occurred in one woman. Two patients developed postoperative hematomas requiring surgical evacuation; one of whom developed urinary retention for 2 weeks. Preoperative urinary symptoms were reported in 35/40 (92%) of women, 8 of whom reported only urge symptoms and experienced complete resolution after Prolift. Resolution of stress urinary incontinence in women who had urodynamic stress incontinence with and without urge symptoms was reported in 60% (12/20) and 20% (1/5), respectively. This represented 62.5% (10/16) and 33.3% (3/9) of women who had total Prolift and posterior Prolift respectively. Average follow‐up periods were 7 and 39 weeks in 38 and 18 women, respectively.Conclusion:  This study shows that Prolift procedure is safe and effective in women with severe pelvic organ prolapse with resolution of most of the bulge and urinary urge symptoms. Total Prolift was effective in the treatment of associated mixed urinary incontinence.
Exogenous vascular endothelial growth factor supplementation can restore the podocyte barrier‐forming capacity disrupted by sera of preeclamptic women
Authors: Henao Daniel E. , Cadavid Ángela P. , Saleem Moin A. 
Published: May 2012

AbstractAim:  To determine whether exogenous vascular endothelial growth factor (VEGF) supplementation to sera from women with preeclampsia can restore the podocyte barrier‐forming capacity disrupted after stimulation with sera from these women.Material and Methods:  Enzyme‐linked immunosorbent assays (ELISAs) for sFlt‐1 (soluble VEGF receptor 1) and VEGF were performed in the sera of preeclamptic and healthy pregnant women. VEGF‐enriched preeclamptic serum was used to evaluate the capacity of such sera to restore the diminished podocyte barrier capacity secondary to stimulation with untreated preeclamptic sera.Results:  Two groups, preeclampsia (n = 15) and healthy (n = 15) pregnant women, were established. Median serum levels (pg/mL) of sFlt‐1 and VEGF were significantly different between groups: 3987 versus 1432 and 6 versus 15.6 (P < 0.001). Altered resistance values of podocytes stimulated with sera from women with preeclampsia were recovered after exogenous VEGF supplementation.Conclusion:  The mean resistance value of cultured podocytes, an indicator of the integrity of the cytoskeleton and the capacity of these cells to form layers, is altered in women with preeclampsia. High levels of sFlt‐1, an inhibitor of VEGF observed in high concentrations in the serum of these patients, could be the underlying cause of this alteration. We provide evidence that exogenous VEGF supplementation of the sera of these women could recover this indicator.
Robotic surgery and standard laparoscopy: A surgical hybrid technique for use in colorectal endometriosis
Authors: Vitobello Domenico , Fattizzi Nicola , Santoro Giuseppe , Rosati Riccardo , Baldazzi Gianandrea , Bulletti Cinzia , Palmara Vittorio 
Published: May 2012

AbstractAim:  The aim of our work was to assess the feasibility and possible benefits of a novel hybrid surgical technique in rectosigmoidal resection in patients with bowel endometriosis.Material and Methods:  A total of seven symptomatic and infertile women with severe bowel endometriosis underwent segmental bowel resection using the da Vinci surgical system and conventional laparoscopy. Statistical analysis was performed by Friedman test for non‐parametric multiple comparisons.Results:  The surgical procedure has a determined short mean operative time (210 min) and short postoperative hospitalization (five days). In 100% of patients, the resected area showed disease‐free margins. Follow‐up, carried out at three, six and 12 months after operation, showed a regression of painful symptoms in all operated patients (100%). Two patients (28.6%) aged ≥ 35 years eventually had natural pregnancies.Conclusion:  To the best of our knowledge, this report is the first concerning the use of a hybrid technique for intestinal resection in severe endometriosis, and comparing our data with that in the literature, its methodological and clinical advantages are evident. Moreover, the complete removal of endometriotic implants seems to offer good results in terms of postoperative fertility, although the study data do not allow us to draw definitive conclusions on the management of fertility.