bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sole abnormalities of chromosome 7 in myeloid malignancies: Spectrum, histopathologic correlates, and prognostic implications
Authors: Hussain Fareeda Taher Nazer , Nguyen Edward P. , Raza Sania , Knudson Ryan , Pardanani Animesh , Hanson Curtis A. , Van Dyke Daniel , Tefferi Ayalew 
Published: April 2012

AbstractAmong 6,565 consecutive abnormal cytogenetic reports at our institution, 3,192 (49%) constituted sole abnormalities, of which 230 (7%) involved chromosome 7: monosomy 7 (n = 98), 7q‐ (n = 51), der(1;7)(q10;p10) (n = 44), balanced translocations (n = 15), ring 7 (n = 13), and 7p‐ (n = 9). The most frequent histopathologic correlates were myelodysplastic syndromes (MDS; 28%), acute myeloid leukemia (AML; 17%), secondary or therapy‐related MDS/AML (13%), primary myelofibrosis (PMF; 7%), and chronic myelomonocytic leukemia (6%). Monosomy 7 was the most frequent in each one of these disease categories except PMF where 7q‐ was more frequent. In primary MDS, patients with der(1;7)(q10;p10) (n = 13), compared to those with monosomy 7 (n = 30) or 7q‐ (n = 15), were less likely (P = 0.04) to display excess blasts or multilineage dysplasia but overall and leukemia‐free survival adjusted for these variables revealed no significant difference between the three groups (P = 0.57 and 0.81, respectively). The current study does not prognostically distinguish monosomy 7 from 7q‐ or der(1;7), in MDS. Am. J. Hematol. 87:684–686, 2012. © 2012 Wiley Periodicals, Inc.
Severe phenotype in MPS II patients associated with a large deletion including contiguous genes
Authors: Brusius‐Facchin Ana Carolina , De Souza Carolina Fischinger Moura , Schwartz Ida Vanessa D. , Riegel Mariluce , Melaragno Maria Isabel , Correia Patrícia , Moraes Lúcia Marques , Llerena Juan , Giugliani Roberto , Leistner‐Segal Sandra 
Published: April 2012

AbstractHunter disease or mucopolysaccharidosis type II (MPS II) is an X‐linked recessive lysosomal disorder caused by the deficiency of iduronate‐2‐sulfatase, which is involved in the catabolism of the glycosaminoglycans (GAGs) heparan and dermatan sulphate. Our aim was to analyze three patients with severe Hunter syndrome that showed a total deletion of the iduronate‐2‐sulphatase (IDS) gene, after exon by exon PCR. DNA was used as a template for PCR synthesis of IDS, FRAXA, FRAXE, and DXS1113 specific amplicons. The DNA analysis for all three patients demonstrated a complete deletion of IDS, FRAXA, and FRAXE contiguous genes. We further performed SNP‐array to delineate the deletion breakpoints and to characterize the deletion extension in the different patients. The results indicated a ∼9.4 Mb deletion in Patient 1, a ∼3.9 Mb deletion of the Xq27.3–Xq28 and a ∼3.1 Mb duplication of the X q28 region in Patient 2 and a ∼41.8 Kb deletion in Patient 3. SNP‐array was shown to be important to map for deletion breakpoints. A comprehensive molecular analysis in patients with Hunter syndrome, especially in the ones presenting the severe form, is important to the understanding of the genetic determinants of the phenotype and for the genetic counseling to be provided to the families. © 2012 Wiley Periodicals, Inc.
Preparation and optical properties of transparent epoxy composites containing ZnO nanoparticles
Authors: Ding Ke Hong , Wang Gen Lin , Zhang Ming 
Published: April 2012

AbstractPolymer nanocomposites are usually made by incorporating dried nanoparticles into polymer matrices. This way not only leads to easy aggregation of nanoparticles but also readily brings about opaqueness for nanocomposites based on functionally transparent polymers. In this letter, transparent ZnO/epoxy nanocomposites with high‐UV shielding efficiency were prepared via two simple steps: first, in situ preparation of zinc hydroxide (Zn(OH)2)/epoxy from the reaction of aqueous zinc acetate (Zn(Ac)2·2H2O) and sodium hydroxide (NaOH) at 30°C in the presence of high‐viscosity epoxy resin; second, thermal treatment of the as‐prepared Zn(OH)2/epoxy hybrid into ZnO/epoxy composites. Optical properties of the resultant ZnO/epoxy nanocomposites were studied using an ultraviolet–visible (UV–vis) spectrophotometer. The nanocomposites containing a very low content of ZnO nanoparticles (0.06 wt %) possessed the optimal optical properties, namely high‐visible light transparency and high‐UV light shielding efficiency. Consequently, the as‐prepared ZnO/epoxy nanocomposites are promising for use as novel packaging materials in lighting emitting diodes technology. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Effect of processing conditions on poly(butylene succinate) foam materials
Authors: Zhang Yihe , Lu Bo , Lv Fengzhu , Guo Wenmin , Ji Junhui , Chu Paul K. , Zhang Changan 
Published: April 2012

AbstractPolymeric foams have many advantages such as light weight, high specific strength, strong energy absorption, as well as good sound and thermal insulation. Unfortunately, most polymeric foams such as polyvinyl chloride foams do not undergo natural degradation and thus giving rise to white pollution to the environment. Here, we report the preparation and properties of poly (butylene succinate) (PBS) foam which is a biodegradable plastic. Ammonium bicarbonate is used as the foaming agent during the formation of the PBS foam by the molding method, and under the optimal conditions of 5 wt % ammonium bicarbonate, 10 MPa pressure, and 5 min pressing time, the pristine PBS foam with the smallest bulk density can be obtained, whereas under the conditions of 5 wt % ammonium bicarbonate, 7.5 MPa pressure, and 7 min pressing time, the toughening PBS foam has the smallest bulk density. Adding talc as a nucleating agent can reduce the bulk density of the foam and in this case, an NH4HCO3 content of 3 wt % yields the best result. Moreover, addition of plasticizers can effectively improve the mechanical properties of the products. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Preparation and properties of polyurethane/multiwalled carbon nanotube nanocomposites by a spray drying process
Authors: Wang Wencai , Jiang Fengdan , Jiang Yi , Lu Yonglai , Zhang Liqun 
Published: April 2012

AbstractA spray drying approach has been used to prepare polyurethane/multiwalled carbon nanotube (PU/MWCNT) composites. By using this method, the MWCNTs can be dispersed homogeneously in the PU matrix in an attempt to improve the mechanical properties of the nanocomposites. The morphology of the resulting PU/MWCNT composites was investigated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM and TEM observations illustrate that the MWCNTs are dispersed finely and uniformly in the PU matrix. X‐ray diffraction results indicate that the microphase separation structure of the PU is slightly affected by the presence of the MWCNTs. The mechanical properties such as tensile strength, tensile modulus, elongation at break, and hardness of the nanocomposites were studied. The electrical and the thermal conductivity of the nanocomposites were also evaluated. The results show that both the electrical and the thermal conductivity increase with the increase of MWCNT loading. In addition, the percolation threshold value of the PU composites is significantly reduced to about 5 wt % because of the high aspect ratio of carbon nanotubes and exclusive effect of latex particles of PU emulsion in dispersion. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Kinetic Limitations of Intracranial Joints in Brachylophosaurus canadensis and Edmontosaurus regalis (Dinosauria: Hadrosauridae), and Their Implications for the Chewing Mechanics of Hadrosaurids
Authors: Cuthbertson Robin S. , Tirabasso Alex , Rybczynski Natalia , Holmes Robert B. 
Published: April 2012

AbstractThe highly specialized tooth morphology and arrangement of the dental battery of hadrosaurids has led to much speculation surrounding the chewing mechanics of this successful group of herbivorous dinosaurs. Pleurokinesis, a long established hypothesis explaining the ornithopod chewing mechanism, proposes a transverse power stroke in hadrosaurids that was accommodated by vertical adduction of the mandible, lateral rotation of the maxilla at the maxilla‐premaxilla joint, lateral rotation of the jugal‐maxilla complex at its contact with the lacrimal, and posterolateral rotation of the quadrate at its contact with the squamosal. A secondary series of movements were also thought to have occurred as a consequence of these primary movements. In this article, the intracranial joint morphology is described for both Brachylophosaurus canadensis and Edmontosaurus regalis and their permissive kinematics are established. Based on this evidence, the movements associated with pleurokinesis are not accommodated in these hadrosaurine dinosaurs. Rather, the movements that seem most likely to have produced the observed dental wear patterns are those associated with the mandible about the jaw joint. The structure of this joint appears well‐suited to have accommodated some translation as well as rotation of the mandible about the quadrate condyle. Three‐dimensional modeling of the alternate mandibular movements reveals that not all the combined labiolingual width of the lingual and buccal facets of the tooth row was involved in the power stroke. Rather, limits on the degree of mandibular long axis rotation suggest that only the lingual facet and the more medial portion of the buccal facet were utilized. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
The Auditory Anatomy of the Minke Whale (Balaenoptera acutorostrata): A Potential Fatty Sound Reception Pathway in a Baleen Whale
Authors: Yamato Maya , Ketten Darlene R. , Arruda Julie , Cramer Scott , Moore Kathleen 
Published: April 2012

AbstractCetaceans possess highly derived auditory systems adapted for underwater hearing. Odontoceti (toothed whales) are thought to receive sound through specialized fat bodies that contact the tympanoperiotic complex, the bones housing the middle and inner ears. However, sound reception pathways remain unknown in Mysticeti (baleen whales), which have very different cranial anatomies compared to odontocetes. Here, we report a potential fatty sound reception pathway in the minke whale (Balaenoptera acutorostrata), a mysticete of the balaenopterid family. The cephalic anatomy of seven minke whales was investigated using computerized tomography and magnetic resonance imaging, verified through dissections. Findings include a large, well‐formed fat body lateral, dorsal, and posterior to the mandibular ramus and lateral to the tympanoperiotic complex. This fat body inserts into the tympanoperiotic complex at the lateral aperture between the tympanic and periotic bones and is in contact with the ossicles. There is also a second, smaller body of fat found within the tympanic bone, which contacts the ossicles as well. This is the first analysis of these fatty tissues' association with the auditory structures in a mysticete, providing anatomical evidence that fatty sound reception pathways may not be a unique feature of odontocete cetaceans. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
Suppression of dendritic cell maturation and T cell proliferation by synovial fluid myeloid cells from mice with autoimmune arthritis
Authors: Egelston Colt , Kurkó Júlia , Besenyei Timea , Tryniszewska Beata , Rauch Tibor A. , Glant Tibor T. , Mikecz Katalin 
Published: April 2012

AbstractObjectiveTo determine whether myeloid cells (such as granulocytes) present in the synovial fluid (SF) of arthritic joints have an impact on adaptive immunity. Specifically, we investigated the effects of SF cells harvested from the joints of mice with proteoglycan‐induced arthritis (PGIA), on dendritic cell (DC) maturation and antigen‐specific T cell proliferation.MethodsWe monitored DC maturation (MHCII and CD86 expression) by flow cytometry upon coculture of DCs with SF cells or spleen myeloid cells from mice with PGIA. The effects of these myeloid cells on T cell proliferation were studied using T cells purified from PG‐specific T cell receptor (TCR)–transgenic (Tg) mice. Phenotype analysis of myeloid cells was performed by immunostaining, reverse transcription–polymerase chain reaction, Western blotting, and biochemical assays.ResultsInflammatory SF cells significantly suppressed the maturation of DCs upon coculture. PG‐TCR–Tg mouse T cells cultured with antigen‐loaded DCs showed dramatic decreases in proliferation in the presence of SF cells. Spleen myeloid cells from arthritic mice did not have suppressive effects. SF cells were unable to suppress CD3/CD28‐stimulated proliferation of the same T cells, suggesting a DC‐dependent mechanism. SF cells exhibited all of the characteristics of myeloid‐derived suppressor cells (MDSCs) and exerted suppression primarily through the production of nitric oxide and reactive oxygen species by granulocyte‐like cells.ConclusionSF in the joints of mice with PGIA contains a population of granulocytic MDSCs that potently suppress DC maturation and T cell proliferation. These MDSCs have the potential to limit the expansion of autoreactive T cells, thus breaking the vicious cycle of autoimmunity and inflammation.
Investigation of SUMO pathway genes in the etiology of nonsyndromic cleft lip with or without cleft palate
Authors: Carta Eloisa , Pauws Erwin , Thomas Anna C. , Mengrelis Konstantinos , Moore Gudrun E. , Lees Melissa , Stanier Philip 
Published: April 2012

AbstractBACKGROUND: SUMO1 has been implicated as having a role in the causation of cleft lip with or without cleft palate (CLP), both directly and through association studies in humans and, perhaps more controversially, in transgenic mouse studies. METHODS: To screen for sequence variants that might be responsible for human CLP, we performed direct DNA sequence analysis in a well‐characterized white European cohort of 192 patients. We screened the genes encoding SUMO1, SUMO2, and SUMO3, as well as the E3 ligases PIAS1 and PIAS2, which are required for sumoylation. Variants were analyzed in a cohort of 192 unaffected white European controls. RESULTS: Only two missense variants were identified, both within SUMO3, however, these were both present in multiple affected individuals and a similar number of controls. Other variants identified, apart from a single synonymous change in PIAS1, were all present within flanking intronic regions distant from splice consensus sites. Moreover, most other variants were previously reported in dbSNP and were shown to be present at a similar frequency in cases and controls. CONCLUSIONS: Our findings indicate that mutations identified in the SUMO‐related genes tested, including three novel coding SNPs, do not directly contribute to the incidence of CLP. Birth Defects Research (Part A), 2012. © 2012 Wiley Periodicals, Inc.
Olive oil supplemented with Coenzyme Q10: Effect on plasma and lipoprotein oxidative status
Authors: Brugè Francesca , Bacchetti Tiziana , Principi Federica , Scarpa Emanuele‐Salvatore , Littarru Gian Paolo , Tiano Luca 
Published: April 2012

AbstractOlive oil consumption is associated with protective cardiovascular properties, including some beneficial modifications in lipoprotein profile and composition. Coenzyme Q10 (CoQ10) exerts a protective effect on plasma lipoproteins. Aim of the study was to investigate whether extra virgin (EV) olive oil enriched with CoQ10 affects CoQ10 levels and oxidative status in plasma and in isolated lipoproteins. Twelve subjects were administered 20 mL olive oil per day for 2 weeks, followed by 2 weeks of olive oil enriched with 20 mg and 2 more weeks with 40 mg of CoQ10. Plasma and isolated lipoproteins were collected in each phase of the study and subsequently analyzed to assess lipid profile, CoQ10 levels, ORAC assay, resistance of lipoproteins to peroxidation and paroxonase 1 activity. Plasma CoQ10 levels significantly increased with the 20 mg (+73%) and 40 mg dose (+170%), while the percentage of oxidized CoQ10 decreased. A significant inverse correlation was found in plasma between percentage of oxidized CoQ10 and total antioxidant capacity. A lower susceptibility of LDL to peroxidation was also found. Finally, a positive correlation was observed between concentration of CoQ10 in HDL and paraoxonase‐1 activity. EV olive oil enriched with both doses of CoQ10 significantly affects its bioavailability and plasma redox status. These changes are associated with a decreased susceptibility of plasma lipoproteins to peroxidation associated with a chain‐breaking antioxidant activity of the formulation. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
An update on advanced glycation endproducts and atherosclerosis
Authors: Del Turco Serena , Basta Giuseppina 
Published: April 2012

AbstractAdvanced glycation endproducts (AGEs) are a group of modified molecular species formed by nonenzymatic reactions between the aldehydic group of reducing sugars with proteins, lipids, or nucleic acids. Formation and accumulation of AGEs are related to the aging process and are accelerated in diabetes. AGEs are generated in hyperglycemia, but their production also occurs in settings characterized by oxidative stress and inflammation. These species promote vascular damage and acceleration of atherosclerotic plaque progression mainly through two mechanisms: directly, altering the functional properties of vessel wall extracellular matrix molecules, or indirectly, through activation of cell receptor‐dependent signaling. Interaction between AGEs and the key receptor for AGEs (RAGE), a transmembrane signaling receptor which is present in all cells relevant to atherosclerosis, alters cellular function, promotes gene expression, and enhances the release of proinflammatory molecules. The importance of the AGE‐RAGE interaction and downstream pathways, leading to vessel wall injury and plaque development, has been amply established in animal studies. Moreover, the deleterious link of AGEs with diabetic vascular complications has been suggested in many human studies. Blocking the vicious cycle of AGE‐RAGE axis signaling may be essential in controlling and preventing cardiovascular complications. In this article, we review the pathogenetic role of AGEs in the development, progression and instability of atherosclerosis, and the potential targets of this biological system for the prevention and treatment of cardiovascular disease. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
Prediction of antimicrobial peptides based on the adaptive neuro‐fuzzy inference system application
Authors: Fernandes Fabiano C. , Rigden Daniel J. , Franco Octavio L. 
Published: April 2012

AbstractAntimicrobial peptides (AMPs) are widely distributed defense molecules and represent a promising alternative for solving the problem of antibiotic resistance. Nevertheless, the experimental time required to screen putative AMPs makes computational simulations based on peptide sequence analysis and/or molecular modeling extremely attractive. Artificial intelligence methods acting as simulation and prediction tools are of great importance in helping to efficiently discover and design novel AMPs. In the present study, state‐of‐the‐art published outcomes using different prediction methods and databases were compared to an adaptive neuro‐fuzzy inference system (ANFIS) model. Data from our study showed that ANFIS obtained an accuracy of 96.7% and a Matthew's Correlation Coefficient (MCC) of 0.936, which proved it to be an efficient model for pattern recognition in antimicrobial peptide prediction. Furthermore, a lower number of input parameters were needed for the ANFIS model, improving the speed and ease of prediction. In summary, due to the fuzzy nature of AMP physicochemical properties, the ANFIS approach presented here can provide an efficient solution for screening putative AMP sequences and for exploration of properties characteristic of AMPs. © 2012 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 98: 280–287, 2012.
A Rapid Access to Novel and Known Benzimidazole Derivatives Using Silica Chloride as a Reusable Catalyst
Authors: Karami Bahador , Ghashghaee Vahideh , Khodabakhshi Saeed 
Published: April 2012

AbstractA new application of silica chloride as an easily available and reusable solid acid catalyst for the synthesis of benzimidazole and its derivatives through the condensation of o‐phenylenediamines and orthoesters under thermal and solvent‐free conditions is described. This novel and eco‐friendly method is very cheap and has many advantages including excellent yields, short reaction time, and simple work‐up procedure.
Synthesis, Crystal Structure and Thermochemical Properties of a One‐Dimensional Chain Complex [Cd(succ)PIP]n
Authors: Li Xia , Zhao Wenjie , Liu Fei , Tan Jinting , Feng Xing , Lu Yingli , Yang Xuwu 
Published: April 2012

AbstractA new complex, [Cd(succ)PIP]n (PIP=2‐phenyl‐imidazo[4,5‐f]1,10‐phenanthroline, H2‐succ=succinate), was synthesized and characterized by X‐ray crystallography, elemental analysis, and TG‐DTG. The results show that the complex crystallizes in an orthorhombic space group Pcca; a=14.065(2) Å, b=9.901(8) Å, c=28.933(2) Å and Z=8. The structure of the complex is one‐dimensional chain [Cd(succ)PIP]n, and each Cd2+ is five‐coordinated by two chelating nitrogen atoms from one PIP ligand, three oxygen atoms from three different succ dianionic ligands to form a distorted trigonal‐bipyramida geometry. The constant‐volume combustion energy of the complex, ΔcU, was determined by an intelligent micro‐rotating‐bomb calorimeter (IMRBC‐type I) at 298.15 K. Then the standard molar enthalpy of combustion, ΔcHm⊖, and the standard molar enthalpy of formation, ΔfHm⊖ have been calculated.
Environmental Friendly Synthesis of Novel Isatin Ketal and Isatin Schiff Base Derivatives Using Michael Addition Reaction under Solvent‐Free Conditions
Authors: Imanzadeh Gholamhassan , Soltanizadeh Zahra , Khodayari Ali , Zamanloo Mohammadreza , Mansoori Yagoub , Salehzadeh Jaber 
Published: April 2012

AbstractAn efficient and simple procedure for the synthesis of novel isatin derivatives is described. Michael addition of aniline Schiff bases of isatin or p‐toluidine Schiff bases of isatin to fumaric esters affords the Michael adduct compounds in good to high yields in the presence of K2CO3 and tetrabutylammonium bromide (TBAB) under solvent‐free conditions. Repeating of this reaction about spiro[1,3‐dioxolane‐2,3′‐indol]‐2′(1′H)‐one, as a Michael donor, in the presence of 1,4‐diazabicyclo[2.2.2]octane (DABCO) gives Michael adducts in remarkable yields under the same conditions.
Synthesis, Structure, and Properties of Two Supramolecular Compounds Based on Silicotungstic Acid and Transition Metal(II) Coordinated Isonicotinic Acid
Authors: Kong Zuping , Wang Chunling , Ren Yuanhang , Gu Min , Hu Yichen , Yue Bin , He Heyong 
Published: April 2012

AbstractTwo compounds, namely [Cd(HINA)2(µ2‐H2O)(H2O)2]2[SiW12O40]·6H2O (1) and [Co(HINA)3(H2O)3][Co(HINA)2(H2O)4][SiW12O40]·2H2O (2), have been synthesized from the aqueous mixture containing H4SiW12O40, isonicotinic acid (HINA), and M(CH3COO)2 (M=Cd and Co). The compounds have been characterized by elemental analysis, IR spectroscopy, TG analysis, and single‐crystal X‐ray diffraction. The dinuclear coordinated cadmium units in compound 1 are linked to form 2D layer parallel to ab plane through π‐π interactions and hydrogen bonds. Compound 2 contains two different types of coordinated metal cations, [Co(HINA)3(H2O)3]2+ and [Co(HINA)2(H2O)4]2+, which construct 2D layer along bc plane through π‐π interactions and hydrogen bonds. In both compounds, the Keggin anions are located inside the channels and cavities formed from stack of the coordinated metal cations, which further achieve the 3D supramolecular structure through hydrogen bonds. The luminescent property of compounds 1 and 2 has been investigated.