bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of oxygen partial pressure on physical properties of nano‐crystalline silver oxide thin films deposited by RF magnetron sputtering
Authors: Narayana Reddy P. , Sreedhar A. , Hari Prasad Reddy M. , Uthanna S. , Pierson J. F. 
Published: June 2011

AbstractNano‐crystalline silver oxide films were deposited on glass and silicon substrates held at room temperature by RF magnetron sputtering of silver target under different oxygen partial pressures. The influence of oxygen partial pressure on the structural, morphological, electrical and optical properties of deposited films was investigated. Varying oxygen partial pressure during the sputter deposition leads to changes of mixed phase of Ag2O and Ag to a single phase of Ag2O and to AgO. The X‐ray diffraction and X‐ray photoelectron spectroscopy results showed the formation of single phase Ag2O with cubic structure at oxygen partial pressures of 2x10‐2 Pa while the films deposited at higher oxygen partial pressure of 9x10‐2 Pa showed the formation of single phase of AgO with monoclinic structure. Raman spectroscopic studies on the single phase Ag2O showed the stretching vibration of Ag‐O bonds. Single‐phase Ag2O films obtained at oxygen partial pressure of 2x10‐2 Pa were nano‐crystalline with crystallite size of 20 nm and possessed an electrical resistivity of 5.2x10‐3 Ωcm and optical band gap of 2.05 eV. The films deposited at higher oxygen partial pressure of 9x10‐2 Pa were of AgO with electrical resistivity of 1.8x10‐2 Ωcm and optical band gap of 2.13 eV. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Tin‐Based Mesoporous Silica for the Conversion of CO2 into Dimethyl Carbonate
Authors: Ballivet‐Tkatchenko Danielle , Bernard Frédéric , Demoisson Frédéric , Plasseraud Laurent , Sanapureddy Sreevardhan Reddy 
Published: June 2011

AbstractSn‐based SBA‐15 was prepared by reacting di‐n‐butyldimethoxystannane with SBA‐15 pretreated with trimethylchlorosilane (TMCS) to cap the external hydroxyl groups. Small‐angle X‐ray diffraction (SXRD), infrared spectroscopy (IR), nitrogen adsorption/desorption, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and inductively coupled plasma atomic emission (ICP‐AES) measurements allow us to propose that the organotin species are located within the pore channels of the mesoporous host. This novel material catalyzes selectively the coupling of CO2 with methanol to dimethyl carbonate (DMC). The reaction time–conversion dependence shows that a turnover number (TON) of 16 can be reached at 423 K under 20 MPa, which is among the highest reported so far in the absence of water traps. Moreover, as the catalytic activity is retained after recycling, even higher values can be obtained on a cumulative basis. A further TON increase is observed with the reaction temperature. Interestingly, the tin‐based SBA‐15 mesoporous material exhibits lower TONs if the TMCS pretreatment is left out. Therefore, the organotin species located outside the channels are far less active than those located within.
Flow cytometric assay using two fluorescent proteins for the function of the internal ribosome entry site of hepatitis C virus
Authors: Shi Guoli , Yagyu Fumihiro , Shimizu Yohko , Shimizu Kazufumi , Oshima Masamichi , Iwamoto Aikichi , Gao Bin , Liu Wenjun , Gao George Fu , Kitamura Yoshihiro 
Published: June 2011

AbstractThe initiation of translation in hepatitis C virus (HCV) occurs at the internal ribosome entry site (IRES) located at the 5′‐end of its genomic RNA. To study the function of HCV IRES, we constructed a reporter plasmid that generates a bicistronic mRNA encoding two fluorescent proteins: cap‐dependent DsRed2 and IRES‐dependent Azami Green (AG). We introduced the plasmid into Huh7.5.1 and HEK293 cells and measured the relative IRES activity from the ratio of AG's signal to DsRed2's in individual cells using flow cytometry. To compare our method and a conventional biochemical method, we constructed a structurally similar reporter in which Renilla and Firefly luciferases replace DsRed2 and AG, respectively. With these systems, we found that the IRES A164G substitution decreased its activity, that interferon alpha affected the IRES activity in a cell type‐specific manner, and that a synthetic micro‐RNA targeting IRES was able to suppress the gene expression. In conclusion, the two methods were comparable in sensitivity in the studies of IRES mutations and host cell types. We discussed the significance of our findings and potential advantage of the cytometric assay: application to the molecular study of the HCV translation and to screening anti‐IRES drugs. © 2011 International Society for Advancement of Cytometry
Automated gene oscillation phase classification for zebrafish presomitic mesoderm cells
Authors: Lu Yanting , Lu Jianfeng , Liu Tianming , Yang Jingyu 
Published: June 2011

AbstractZebrafish somitogenesis is governed by a segmentation clock that generates oscillations of gene expression in the zebrafish presomitic mesoderm (PSM) cells. The segmentation clock causes cells to undergo repeated cycles of transcriptional activation and repression, which can be divided into eight phases based on their distinct mRNA co‐localizations. Recognizing different gene oscillation phases of cells is important in zebrafish research, but manual analysis is time‐consuming and difficult. In this article, an effective automated gene oscillation phase classification framework is established for zebrafish PSM cell images. The framework consists of three major steps: (1) identify the individual cells by a two‐stage segmentation procedure; (2) extract multiple features on each cell patch to measure the subcellular mRNA distribution; (3) employ a support vector machine (SVM) with a combined kernel to complete feature fusion and classification. To evaluate the effectiveness of this framework, a dataset containing 2,227 cell samples is constructed. Experimental results on this dataset indicate that our approach can achieve reasonably good performance for this gene oscillation classification problem. The feature sets NF9 and SPIN introduced in this article have proved to be superior to other cell features in this problem. Besides, the kernel fusion method used in the third step provides a way to combine heterogeneous features together, i.e., numerical feature set and histogram‐based feature set, and classification performance with the combined kernel is better than single feature. © 2011 International Society for Advancement of Cytometry
Evaluation of CINtec PLUS® testing as an adjunctive test in ASC‐US diagnosed SurePath® preparations
Authors: Edgerton Neil , Cohen Cynthia , Siddiqui Momin T. 
Published: June 2011

AbstractThe CINtec PLUS® system is an immunohistochemical cocktail composed of antibodies against p16INK4a (surrogate of HPV infection) and Ki‐67 (proliferation marker) meant to improve the sensitivity and specificity for detecting high‐grade dysplasia (HGD). In the presence of dysplasia, a red chromogen marks Ki‐67 expression in the nucleus and a brown chromogen marks cytoplasmic p16INK4a expression. Only cells showing dual staining are interpreted as positive. This retrospective study examined the performance of CINtec PLUS testing when performed on ASC‐US diagnosed samples. Comparison was made to high‐risk HPV DNA test results and colposcopic biopsy results. Technical considerations in the interpretation of this immunohistochemical stain are additionally discussed. CINtec PLUS showed modest sensitivity (64%) and specificity (53%) in identifying the presence of HGD at surgical biopsy. Positive and negative predictive values for HGD were 28% and 83%, respectively. HR‐HPV DNA test yielded sensitivity of 100% and specificity of 21%. During interpretation, squamous metaplasia and endocervical cells were seen to show individual staining for p16INK4a or Ki‐67. Individual staining, when present within three dimensional cellular groups common to SurePath® preparations, can be time‐intensive to interpret necessitating thoughtful examination at high power. The Pap test with HR‐HPV DNA testing is a highly sensitive test. A specific test is needed to prevent false positives and over treatment. The CINtec® system provides a modest increase in specificity beyond HR‐HPV DNA testing. Future study of its appropriateness and cost‐ffectiveness in a treatment algorithm are warranted. Diagn. Cytopathol. 2013. © 2011 Wiley Periodicals, Inc.
Z‐axis video for cytology database is a useful tool for the case presentation prior to the cytology training workshop
Authors: Yamashiro Katsushige , Shinohara Toshiya , Mitsuhashi Tomoko , Sugimura Takuya , Taira Kiyomi , Azuma Manabu , Okuyama Dai , Nakajima Manami , Takeda Hiroko , Suzuki Hiroaki 
Published: June 2011

AbstractWe have built a database on the Internet managing z‐axis video for cytology (Zavic), and report on a new style of case discussion supported by the Zavic database. Z‐axis video for cytology is a movie file derived from the video recording of a microscopic field with changes in the focus. We used it for the case presentation of EUS‐FNA of pancreatic lesions on the Internet prior to a training workshop. The attendees were asked to observe the Zavic and to make diagnoses of 20 cases before the workshop. Fourteen attendees also observed lesions under a microscope on that day, and the results were compared with those of Zavic observation. The evaluation of the Zavic database (DB) was surveyed by a questionnaire. The average number of accurate diagnoses by 46 Zavic observers was 10.8. These accuracies for those who observed both the Zavic and glass slides were 11.57 and 11.43, respectively, for the videos and slides. Compared with Zavic observation alone, the diagnoses with glass slide observation were shifted to a correct diagnosis in two cases, but were shifted to an incorrect diagnosis in two cases. Approximately 60% of Zavic observers replied in the questionnaire that the movies on Zavic DB started to play within 3–4 seconds after clicking the play button. We successfully carried out the new style of case discussion supported by the Zavic DB. It was evaluated favorably by many attendees, who were psychologically still dependent on the glass slide observation. Diagn. Cytopathol. 2013. © 2011 Wiley Periodicals, Inc.
Epithelial membrane transporters expression in the developing to adult mouse vomeronasal organ and olfactory mucosa
Authors: Merigo Flavia , Mucignat‐Caretta Carla , Cristofoletti Mirko , Zancanaro Carlo 
Published: June 2011

AbstractTo contribute clarifying mechanisms operating in nose chemosensory epithelia and their developmental patterns, we analyzed the expression of different epithelial membrane transporters as well as the Clara cell secretory protein, CC26 in the olfactory, vomeronasal organ (VNO), and respiratory epithelia of embryonic (E13–E19) and postnatal (P1–P60) mice by means of immunohistochemistry and reverse transcriptase‐polymerase chain reaction. Results showed that CC26, cAMP‐activated chloride channel (CFTR), and the water channel protein aquaporin 2, 3, 4, and 5 (AQP2, AQP3, AQP4, and AQP5) are expressed in developing to adult chemosensory epithelia with differential timing; moreover, their pattern of expression is not identical in VNO and olfactory epithelia as well as the corresponding associated glands; co‐localization experiments using olfactory marker protein showed that CFTR, CC26, and AQP4 are not expressed in olfactory neurones. CFTR is expressed in sustentacular cells of the VNO and olfactory epithelium as well as blood vessels of the underlying mucosa, and VNO (but not Bowman's) glands; a similar pattern (excluding blood vessels) is present for AQP2; AQP4 is found in the two chemosensory epithelia and in Bowman's glands. AQP3 is expressed in the olfactory epithelium and the associated Bowman's glands, but not in the VNO chemosensory epithelium and glands. AQP5 is expressed in the olfactory epithelium and both Bowman's and VNO glands. These results indicate that water/ions handling as well as antioxidant mechanisms operating at the surface and/or inside the nose chemosensory epithelia start developing in utero and are maintained up to sexual maturity. © 2011 Wiley Periodicals, Inc. Develop Neurobiol 71: 854–869, 2011
Internalized weight bias in obese patients with binge eating disorder: Associations with eating disturbances and psychological functioning
Authors: Durso Laura E. , Latner Janet D. , White Marney A. , Masheb Robin M. , Blomquist Kerstin K. , Morgan Peter T. , Grilo Carlos M. 
Published: June 2011

AbstractObjective:Widespread bias against obese individuals may lead to the internalization of weight bias in obese persons. This study examined correlates of internalized weight bias (IWB) in obese patients with binge eating disorder (BED).Method:One hundred treatment‐seeking obese patients with BED were administered with the eating disorders examination interview and questionnaires assessing IWB, fat phobia, depression, and self‐esteem.Results:The mean IWB score in this group of patients with BED was significantly greater than the mean IWB score observed previously in a community sample of overweight adults. IWB was positively associated with eating disorder psychopathology, fat phobia, and depression, and negatively associated with self‐esteem. IWB made significant independent contributions to the variance in eating disorder psychopathology even after accounting for fat phobia, depression, and self‐esteem.Discussion:Treatment‐seeking obese patients with BED demonstrate high levels of IWB. IWB may contribute to the variance in eating disorder psychopathology in BED patients, beyond the contributions of fat phobia, depression, and self‐esteem. © 2011 by Wiley Periodicals, Inc. Int J Eat Disord 2012
Synthesis and Structural Characterization of Copper(I), Silver(I) and Gold(I) Complexes with Pyrimidine‐2‐thionato Ligands and their Adducts with Phosphanes
Authors: Rodríguez Antonio , Sousa‐Pedrares Antonio , García‐Vázquez José A. , Romero Jaime , Sousa Antonio 
Published: June 2011

AbstractHomoleptic copper and silver compounds of a series of pyrimidine‐2‐thionato derivatives (RpymS–; R = 4,6‐Me2,5‐Et; 4‐CF3; 4,6‐CF3,Ph) have been synthesized by electrochemical oxidation of anodic metal (copper or silver) in a cell containing an acetonitrile solution of the appropriate thione and a small amount of tetraethylammonium perchlorate as electrolytic carrier. Subsequent reaction of these pyrimidine‐2‐thionato compounds with triphenylphosphane or bis(diphenylphosphanyl)methane (dppm) in acetonitrile allowed the synthesis of the corresponding heteroleptic complexes. The reaction of [Au(PPh3)Cl] with the corresponding pyrimidine‐2‐thione in ethanol in the presence of triethylamine gave [Au(4,6‐Me2,5‐EtpymS)(PPh3)] and [Au(4‐CF3pymS)(PPh3)] in good yields. The compounds were characterized by microanalysis, IR spectroscopy, FAB‐MS and, when sufficientlysoluble, 1H, 13C and 31P NMR spectroscopy. Compounds [Cu6(4,6‐CF3PhpymS)6] (1), [Ag6(4,6‐Me2,5‐EtpymS)6]·2MeOH·3H2O (2), [Cu2(4‐CF3pymS)2(dppm)2]·2CH3CN (3), [Cu(4‐CF3pymS)(PPh3)2] (4) and [Au(4‐CF3pymS)(PPh3)] (5) were also characterized by X‐ray diffraction.
Synthesis and Structural Characterization of Bis(salicylaldiminato)magnesium Complexes of Varying Aggregation and Coordination State
Authors: Quinque Geoffrey T. , Oliver Allen G. , Rood Jeffrey A. 
Published: June 2011

AbstractA series of salicylaldiminato ligands [C6H5N=CHC6H4OH (1L), 2,6‐iPrC6H3N=CHC6H4OH (2L), and 2,6‐iPrC6H3N=CH‐3,5‐tBu2C6H2OH (3L)] were treated with Bu2Mg in the presence of the appropriate solvent system to yield the crystalline compounds [(1L6Mg3)·thf] (1), [(2L2Mg·thf)] (2), and [3L2Mg] (3). The products were characterized by 1H and 13C NMR spectroscopy and single‐crystal X‐ray diffraction. X‐ray crystallographic analysis revealed that 1 adopts a unique trimeric aggregation state consisting of six‐coordinate magnesium centers. Substitution of the ligand backbone resulted in the formation of the monomeric species 2 and 3. X‐ray crystallographic analyses revealed 2 as a five‐coordinate, distorted square‐pyramidal magnesium complex and 3 as a four‐coordinate, distorted tetrahedral species. Inspection of the metrical parameters in 1–3 indicates a decrease in the Mg–O and Mg–N bond lengths and an increase in the N–Mg–O bite angle with a decreasing coordination number at magnesium.
1,2‐Carbagerma‐closo‐dodecaborate as a Germanium Ligand in Coordination Chemistry – Synthesis, Structure and Reactivity
Authors: Wagenpfeil Anita , Nickl Claudia , Schubert Hartmut , Eichele Klaus , Fox Mark A. , Wesemann Lars 
Published: June 2011

AbstractA much improved synthesis for the carbagerma‐closo‐dodecaborate anion [GeCB10H11]– is described in the form of [Bu3NH][GeCB10H11] (1). In reactions with transition metal electrophiles, three transition metal complexes {[Bu3NH][(C6H6)Ru(Cl)2(GeCB10H11)] (2), [Bu3NH][Cp*Ir(Cl)(GeCB10H11)2] (3) and [Me3NH][(PPh3)2Ir(CO)(GeCB10H11)2] (4)} with metal–germanium bonds were synthesized. The carbagermaborate anion, in the form of the salt [Et3NH][GeCB10H11], and the coordination compounds 2–4 were structurally characterized by single‐crystal X‐ray diffraction. Computations were carried out for the anions in 1–4 to aid NMR assignments.
Alkoxymagnesium Iodide Complexes
Authors: Haiss Peter , Kuhn Annette , Kuhn Norbert , Maichle‐Mößmer Cäcilia , Laufer Stefan , Steimann Manfred , Zeller Klaus‐Peter 
Published: June 2011

AbstractThe complexes R(Ph)MeCOMg(Et2O)I [1, R = Me (a), Ph (b)] were obtained by the reaction of R(Ph)C=O with MeMgI in diethyl ether in good yields. Crystallisation of 1 from acetonitrile resulted in single crystals of di‐ and trinuclear complexes I2(μ‐OCPhMe2)2Mg(MeCN)4 (2) and {2[I(MeCN)Mg(μ‐OCPh2Me)2Mg(MeCN)I] (3) × [I(MeCN)Mg(μ‐OCPh2Me)2Mg(μ‐OCPh2Me)2Mg(MeCN)I] (4) × 2CH2Cl2} in which the metal centres are linked by alkoxy substituents. The thermal decomposition of the complexes 1 was studied by successive detection of the EI mass spectra of diethyl ether, the olefins 5, the tertiary alcohols 6, and the formal olefin dimers 7.
Fat‐soluble bioactives in nuts
Authors: Alasalvar Cesarettin , Pelvan Ebru 
Published: June 2011

AbstractThe levels and compositions of fat‐soluble bioactives such as fatty acids, tocols, phytosterols, and sphingolipids present in 12 nut oils (almond, Brazil nut, cashew, chestnut, hazelnut, heartnut, macadamia, peanut, pecan, pine nut, pistachio, and walnut) are reported and compared. Meeting the recommended daily intake of vitamin E and phytosterols by consuming nuts is discussed. The health benefits of fat‐soluble bioactives in nuts have been thoroughly reviewed. Research findings from over 88 references, many of which published only within the last 10 years, have been compiled and reported.
Hydrolysis and Radiation Stability of m‐Xylylene Bis‐diglycolamide: Synthesis and Quantitative Study of Degradation Products by HPLC–APCI+
Authors: Galán Hitos , Murillo María Teresa , Sedano Rosa , Núñez Ana , de Mendoza Javier , González‐Espartero Amparo , Prados Pilar 
Published: June 2011

AbstractFor nuclear hydrometallurgical separation process development, it is necessary to demonstrate the stability of the extracting systems, since it is well known that radio‐ and hydrolytic degradation leads to undesirable effects, such as a decrease in selectivity, poorer phase separation and third‐phase formation. Recently, we have developed a new family of bis‐diglycolamide (bis‐DGA) molecules with high distribution coefficients (D) for EuIII over AmIII. One of these bis‐DGA extractants, namely, compound 1, showed high distribution coefficients even under gamma irradiation at 1000 kGy with external 60Co sources. We report herein a detailed account on the stability of 1 against radio‐ and hydrolysis. We have also identified and quantified the sub‐products formed during the irradiation process. Qualitative and quantitative analyses of irradiated 1 were performed by HPLC–MS, indicating the presence of seventeen degradation compounds. All fragments (2–18) were identified and synthesized independently. To complete this study, the AnIII and LnIII extraction properties of these fragments were assessed under the same experimental conditions as those used to evaluate the AnIII and LnIII extraction by irradiated 1. Despite the significance of a decrease in the concentration of 1, Am/Eu D values are still quite high. This means that at least some degradation products also act as efficient extractants. It is relevant to remark that two of the major degradation products (compounds 3 and 11) are stable to radiation and showed high D values for AmIII and EuIII extraction.
Selective Synthesis of New Fluorinated Alicyclic β‐Amino Ester Stereoisomers
Authors: Kiss Loránd , Forró Enikő , Fustero Santos , Fülöp Ferenc 
Published: June 2011

AbstractNew fluorinated alicyclic β‐amino ester stereoisomers with a cyclohexene or cyclohexane skeleton were prepared from cis‐ or trans‐2‐aminocyclohex‐3‐enecarboxylic acids in five or six steps through a regio‐ and stereoselective hydroxylation and hydroxy–fluorine exchange. Fluorinated aminoester enantiomers were synthesized from enantiopure cis‐ or trans‐2‐aminocyclohexenecarboxylic acid (prepared byenzymatic resolution of the racemic substances).
Voltammetry of Mn(III) Porphyrin in Trihexyl(tetradecyl)‐phosphonium Tris(pentafluoroethyl)trifluorophosphate Supported Toluene in Contact with an Aqueous Electrolyte
Authors: Adamiak Wojciech , Shul Galyna , Rozniecka Ewa , Satoh Masanori , Chen Jingyuan , Opallo Marcin 
Published: June 2011

AbstractVoltammetry of manganese tetraphenylporphyrinato chloride in trihexyl(tetradecyl)phosphonium tris(pentafluoroethyl)trifluorophosphate solution in toluene reveals its unexpectedly low diffusion coefficient. UV‐vis spectra confirm significant Cl− exchange with large phosphate anion at the axial position of the complex. Experiments performed with a glassy carbon electrode covered by a liquid film of the same solution and immersed in an aqueous electrolyte solution show a peak potential dependence on the nature and concentration of the aqueous electrolyte anion described by a Nernst type equation. Electron transfer is followed by anion expulsion where the anion effect results from the lability of the axial coordination site of the porphyrin and from spontaneous ion exchange.
Improved method for immunostaining of mucin separated by supported molecular matrix electrophoresis by optimizing the matrix composition and fixation procedure
Authors: Matsuno Yu‐Ki , Dong Weijie , Yokoyama Seiya , Yonezawa Suguru , Saito Takuro , Gotoh Mitsukazu , Narimatsu Hisashi , Kameyama Akihiko 
Published: June 2011

AbstractMucins are a family of heavily glycosylated high molecular mass proteins that have great potential as novel clinical biomarkers for the diagnosis of various malignant tumors. Supported molecular matrix electrophoresis (SMME) is a new type of membrane electrophoresis that can be used to characterize mucins. In SMME, mucins migrate in a molecular matrix supported by membrane materials. Here, we have developed an immunostaining method for the identification of SMME‐separated mucins. The novel method involves stably fixing the mucins onto the SMME membrane and optimizing the molecular matrix for the fixation process. We applied this technique for the detection of MUC1 produced from three cancer cell lines (T47D, HPAF‐II and BxPC3) and also analyzed their O‐linked glycans by mass spectrometry. Our results revealed that properties of the MUC1 molecules from the three cell lines are different in terms of migrating position in SMME and glycan profile. The present method allows simple and rapid characterization of mucins in terms of both glycans and core proteins. The method will be a useful tool for the exploration of mucin alterations associated with various diseases such as cancer.