bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts
Authors: Rudman-Melnick Valeria , Vanhoutte Davy , Stowers Kaitlynn , Sargent Michelle , Adam Mike , Ma Qing , Perl Anne Karina T. , Miethke Alexander G. , Burg Ashley , Shi Tiffany , Hildeman David A. , Woodle E. Steve S. , Kofron J. Matthew , Devarajan Prasad 
Published: May 2024

AbstractFibrosis is a common outcome of numerous pathologies, including chronic kidney disease (CKD), a progressive renal function deterioration. Current approaches to target activated fibroblasts, key effector contributors to fibrotic tissue remodeling, lack specificity. Here, we report Gucy1α1 as a specific kidney fibroblast marker. Gucy1α1 levels significantly increased over the course of two clinically relevant murine CKD models and directly correlated with established fibrosis markers. Immunofluorescent (IF) imaging showed that Gucy1α1 comprehensively labelled cortical and medullary quiescent and activated fibroblasts in the control kidney and throughout injury progression, respectively. Unlike traditionally used markers platelet derived growth factor receptor beta (Pdgfrβ) and vimentin (Vim), Gucy1α1 did not overlap with off-target populations such as podocytes. Notably, Gucy1α1 labelled kidney fibroblasts in both male and female mice. Furthermore, we observed elevated GUCY1α1 expression in the human fibrotic kidney and lung. Studies in the murine models of cardiac and liver fibrosis revealed Gucy1α1 elevation in activated Pdgfrβ-, Vim- and alpha smooth muscle actin (αSma)-expressing fibroblasts paralleling injury progression and resolution. Overall, we demonstrate Gucy1α1 as an exclusive fibroblast marker in both sexes. Due to its multiorgan translational potential, GUCY1α1 might provide a novel promising strategy to specifically target and mechanistically examine fibroblasts.
Structural and biochemical insights on the mechanism of action of the clinical USP1 inhibitor, KSQ-4279
Authors: Rennie ML , Gundogdu M , Arkinson C , Liness S , Frame S , Walden H 
Published: May 2024

AbstractDNA damage triggers cell signalling cascades that mediate repair. This signalling is frequently dysregulated in cancers. The proteins that mediate this signalling are potential targets for therapeutic intervention. Ubiquitin-specific protease 1 (USP1) is one such target, with small molecule inhibitors already in clinical trials. Here we use biochemical assays and cryo-electron microscopy (cryo-EM) to study the clinical USP1 inhibitor, KSQ-4279 (RO7623066), and compare this to the well-established, tool compound, ML323. We find that KSQ-4279 binds to the same cryptic site of USP1 as ML323 but disrupts the protein structure in subtly different ways. Inhibitor binding drives a substantial increase in thermal stability of USP1, which may be mediated through the inhibitors filling a hydrophobic tunnel in USP1. Our results contribute to the understanding of the mechanism of action of USP1 inhibitors at the molecular level.
Structural insights into transcription regulation of the global virulence factor PhoP fromMycobacterium tuberculosis
Authors: Shi Jing , Song Qian , Feng Zhenzhen , Wen Aijia , Liu Tianyu , Xu Liqiao , Ye Zonghang , Xu Simin , Gao Fei , Xiao Liuxiang , Zhu Jiapeng , Das Kalyan , Zhao Guoping , Feng Yu , Lin Wei 
Published: May 2024

AbstractMycobacterium tuberculosis(Mtb), remaining as the leading cause of the worldwide threat Tuberculosis, relies heavily on its transcriptional reprogramming of diverse stress genes to swiftly adapt to adverse environments and ensure infections. The global virulence factor PhoP plays a pivotal role in coordinating transcription activation or repression of the essential phosphate-nitrogen metabolic remodeling genes. However, what defines PhoP to deferentially act as an activator or a repressor remains largely unexplored. Here, we determine one cryo-EM structure ofMtbRNAP-promoter open complex, three cryo-EM structures of PhoP-dependent transcription activation complexes (PhoP-TACs) consisting ofMtbRNA polymerase (RNAP), different number of PhoP molecules binding to different types of well-characterized consensus promoters, and one cryo-EM structure ofMtbPhoP-dependent transcription repression complex (PhoP-TRC) comprising ofMtbRNAP, PhoP, the nitrogen metabolism regulator GlnR and their co-regulated promoter. Structural comparisons reveal phosphorylation of PhoP is required for stabilization of PhoP-TACs, PhoP specifically recognizes promoters as novel tandem dimers and recruits RNAP through extensively interacting with its conserved β flap and σAR4 domains. Strikingly, the distinct promoter spacer length and PhoP-GlnR interactions in PhoP-TRC constrain the upstream DNA into a distinct topology and retain PhoP in a novel ‘dragging repression mode’. Collectively, these data highlight the dual regulatory mechanisms of PhoP-dependent transcription regulation in governing stress adaptation. These findings provide structural basis for developing potential anti-tuberculosis drugs and/or interventions.
Preferential binding of ADP-bound mitochondrial HSP70 to the nucleotide exchange factor GRPEL1 over GRPEL2
Authors: Manjunath Pooja , Stojkovič Gorazd , Konovalova Svetlana , Wanrooij Sjoerd , Koski Kristian , Tyynismaa Henna 
Published: May 2024

ABSTRACTHuman nucleotide exchange factors GRPEL1 and GRPEL2 play pivotal roles in the ADP-ATP exchange within the protein folding cycle of mitochondrial HSP70 (mtHSP70), a crucial chaperone facilitating protein import into the mitochondrial matrix. Studies in human cells and mice have indicated that while GRPEL1 serves as an essential co-chaperone for mtHSP70, GRPEL2 has a role regulated by stress. However, the precise structural and biochemical mechanisms underlying the distinct functions of the GRPEL proteins have remained elusive. In our study, we present evidence revealing that ADP-bound mtHSP70 exhibits remarkably higher affinity for GRPEL1 compared to GRPEL2, with the latter experiencing a notable decrease in affinity upon ADP binding. Utilizing Alphafold modelling, we propose that the interaction between GRPEL1 and mtHSP70 can induce the opening of the nucleotide binding cleft of the chaperone, thereby facilitating the release of ADP, whereas GRPEL2 lacks this capability. This study elucidates how ADP binding to mtHSP70 enhances its affinity for GRPEL1, contrasting with its interaction with GRPEL2. Additionally, our findings suggest that the redox-regulated Cys87 residue in GRPEL2 does not play a role in dimerization but rather reduces its affinity for mtHSP70. Our findings on the structural and functional disparities between GRPEL1 and GRPEL2 may have implications for mitochondrial protein folding and import processes under varying cellular conditions.
Likelihood-based interactive local docking into cryo-EM maps in ChimeraX
Authors: Read Randy J. , Pettersen Eric F. , McCoy Airlie J. , Croll Tristan I. , Terwilliger Thomas C. , Poon Billy K. , Meng Elaine C. , Liebschner Dorothee , Adams Paul D. 
Published: May 2024

AbstractThe interpretation of cryo-EM maps often includes the docking of known or predicted structures of the components, which is particularly useful when the map resolution is worse than 4 Å. Although it can be effective to search the entire map to find the best placement of a component, the process can be slow when the maps are large. However, frequently there is a well-founded hypothesis about where particular components are located. In such cases, a local search using a map subvolume will be much faster because the search volume is smaller, and more sensitive because optimizing the search volume for the rotation search step enhances signal-to-noise. A Fourier-space likelihood-based local search approach, based on the previously-publishedem_placementsoftware, has been implemented in the newemplace_localprogram. Tests confirm that the local search approach enhances speed and sensitivity of the computations. An interactive graphical interface in the ChimeraX molecular graphics program provides a convenient way to set up and evaluate docking calculations, particularly in defining the part of the map into which the components should be placed.SynopsisLikelihood-based cryo-EM docking using ouremplace_localsoftware is faster and more sensitive than our related software,em_placement, when the approximate location of a component is known, and is available conveniently through a plugin to the ChimeraX visualization software.
Se
Inter-Diffusion Limits Absorber Layer Grain Growth in
CdSe
-
CdTe
Photovoltaics
Authors: Altamimi T.F.S. , Leaver J.F. , Durose K. , Major J.D. , Mendis B.G. 
Published: May 2024

Diffusion of Se from the CdSe window layer into the CdTe absorber improves the short circuit current density by narrowing the band gap and increasing the carrier lifetime. Thicker CdSe layers, however, show a dramatic loss in photocurrent collection due to Se over-alloying. Electron microscopy investigations show that this decrease in performance is due to the formation of small grains (∼783 nm average diameter), which exhibit grain boundary porosity in the Se inter-diffusion region. The larger grain boundary area and void free surfaces give rise to higher levels of nonradiative recombination, and therefore, a lower photocurrent. It is proposed that the small grain size is due to a drag force exerted by segregated Se solute atoms on a moving grain boundary, while faster Se diffusion along the grain boundaries results in vacancy build up and porosity due to the Kirkendall effect. The results indicate that the device processing conditions must be carefully controlled such that the negative effects of Se alloying (i.e., smaller grains, Kirkendall voids) do not undermine its benefits.

Published by the American Physical Society
2024
Synthesis, characterization and structural analysis of complexes from 2,2′:6′,2′′-terpyridine derivatives with transition metals
Authors: Fajardo Daniel A. , Arteaga Danny , Ellena Javier , Santiago Pedro H. O. , D'Vries Richard F. , Lenis Luis Alberto 
Published: May 2024

The synthesis and structural characterization of three families of coordination complexes synthesized from 4′-phenyl-2,2′:6′,2′′-terpyridine (8, Ph-TPY), 4′-(4-chlorophenyl)-2,2′:6′,2′′-terpyridine (9, ClPh-TPY) and 4′-(4-methoxyphenyl)-2,2′:6′,2′′-terpyridine (10, MeOPh-TPY) ligands with the divalent metals Co2+, Fe2+, Mn2+ and Ni2+ are reported. The compounds were synthesized from a 1:2 mixture of the metal and ligand, resulting in a series of complexes with the general formula [M(R-TPY)2](ClO4)2 (where M = Co2+, Fe2+, Mn2+ and Ni2+, and R-TPY = Ph-TPY, ClPh-TPY and MeOPh-TPY). The general formula and structural and supramolecular features were determinated by single-crystal X-ray diffraction for bis(4′-phenyl-2,2′:6′,2′′-terpyridine)nickel(II) bis(perchlorate), [Ni(C21H15N3)2](ClO4)2 or [Ni(Ph-TPY)2](ClO4)2, bis[4′-(4-methoxyphenyl)-2,2′:6′,2′′-terpyridine]manganese(II) bis(perchlorate), [Mn(C22H17N3O)2](ClO4)2 or [Mn(MeOPh-TPY)2](ClO4)2, and bis(4′-phenyl-2,2′:6′,2′′-terpyridine)manganese(II) bis(perchlorate), [Mn(C21H15N3)2](ClO4)2 or [Mn(Ph-TPY)2](ClO4)2. In all three cases, the complexes present distorted octahedral coordination polyhedra and the crystal packing is determined mainly by weak C—H...π interactions. All the compounds (except for the Ni derivatives, for which FT–IR, UV–Vis and thermal analysis are reported) were fully characterized by spectroscopic (FT–IR, UV–Vis and NMR spectroscopy) and thermal (TGA–DSC, thermogravimetric analysis–differential scanning calorimetry) methods.
Gender and organizational commitment: evidence from a nationwide survey in Poland
Authors: Adamchik Vera , Sedlak Piotr 
Published: May 2024

Purpose The study examines whether affective organizational commitment and its drivers differ between Polish female and male employees.Design/methodology/approach Our proprietary data are from ongoing surveys conducted by a major Polish HR consulting firm. The nationwide survey of nearly 3,000 Polish workers in 2020 constitutes the data set in this analysis. Regression analysis is applied to analyze the relationship between organizational commitment, gender and other variables.Findings The study provides support for the job model, that is, women and men have similar levels of commitment once all other factors are controlled. Although the results show that, ceteris paribus, the organizational commitment of women is statistically significantly higher than that of men, the effect size is trivial in practical terms. The study also discloses the fact that the determinants of organizational commitment of men and women are similar, thus refuting a commonly held notion about gendered job attribute preferences. Support for gender as a moderator between organizational commitment and its antecedents is not found. COVID-19-related work adjustments do not seem to have affected the commitment of Polish male and female workers to their employers.Originality/value The study adds to the scarce empirical literature on organizational commitment in Poland. To date, only a small number of such studies exist for Poland, and all of them use small homogeneous samples and limited questionnaires. The results are of value to researchers as well as HR managers seeking to improve long-term commitment to organizations.
Job content plateau and career commitment: the mediating role of developmental i-deals and the moderating role of proactive personality
Authors: Jena Biswa Prakash , Choudhary Archana , Pal Manas Kumar , Misra Siddharth 
Published: May 2024

PurposeGiven the detrimental effects of job content plateau, the paper aims to study the impact of job content plateau on employees’ career commitment. In doing so, the authors examine whether the lapses in job content plateau can be addressed through developmental i-deals. A final purpose is to examine whether proactive employees are better positioned to obtain work arrangements that help them develop and remain committed to their careers.Design/methodology/approachData were collected from full-time working executives employed in different organizations. These executives enrolled in a part-time MBA program. Data was collected at different time points and analyzed using the process macro (Preacher and Hayes, 2004).FindingsThe results suggest that developmental i-deals mediated the relationship between job content plateau and career commitment. In addition, proactive employees were better disposed to seal the deal and develop themselves – helping them to stay committed to their careers.Originality/valuePrior studies highlight the negative consequences of job content plateau because it does not provide avenues to learn and develop. This paper addresses the gap in locating opportunities to learn and develop (an aspect that was missing in the job content plateau) through developmental i-deals. First, the study helps answer how to address learning gaps in jobs. Second, who can capitalize on their efforts once the organization sponsors learning opportunities.
A modified spatial house price to income ratio and housing affordability drivers study: using the post-LASSO approach
Authors: Wang Qifeng , Lin Bofan , Tan Consilz 
Published: May 2024

Purpose
The purpose of this paper is to develop an index for measuring urban house price affordability that integrates spatial considerations and to explore the drivers of housing affordability using the post-least absolute shrinkage and selection operator (LASSO) approach and the ordinary least squares method of regression analysis.

Design/methodology/approach
The study is based on time-series data collected from 2005 to 2021 for 256 prefectural-level city districts in China. The new urban spatial house-to-price ratio introduced in this study adds the consideration of commuting costs due to spatial endowment compared to the traditional house-to-price ratio. And compared with the use of ordinary economic modelling methods, this study adopts the post-LASSO variable selection approach combined with the k-fold cross-test model to identify the most important drivers of housing affordability, thus better solving the problems of multicollinearity and overfitting.

Findings
Urban macroeconomics environment and government regulations have varying degrees of influence on housing affordability in cities. Among them, gross domestic product is the most important influence.

Research limitations/implications
The paper provides important implications for policymakers, real estate professionals and researchers. For example, policymakers will be able to design policies that target the most influential factors of housing affordability in their region.

Originality/value
This study introduces a new urban spatial house price-to-income ratio, and it examines how macroeconomic indicators, government regulation, real estate market supply and urban infrastructure level have a significant impact on housing affordability. The problem of having too many variables in the decision-making process is minimized through the post-LASSO methodology, which varies the parameters of the model to allow for the ranking of the importance of the variables. As a result, this approach allows policymakers and stakeholders in the real estate market more flexibility in determining policy interventions. In addition, through the k-fold cross-validation methodology, the study ensures a high degree of accuracy and credibility when using drivers to predict housing affordability.
Artificial intelligence – talent acquisition in HEIs recruitments
Authors: R. Vedapradha , R. Hariharan , E. Sudha , V. Divyashree 
Published: May 2024

PurposeThe current research study aims to examine the application feasibility and impact of artificial intelligence (AI) among higher educational institutions (HEIs) in talent acquisitions (TA).Design/methodology/approachA systematic sampling method was adopted to collect the responses from the 385 staff working across the various levels of management in HEIs in metropolitan cities in India. JAMOVI & SmartPLS 4 were applied to validate the hypothesis by performing the simple percentage analysis and structural equation modelling. The demographic and construct variables considered were adoption, actual usage, perceived usefulness, perceived ease of use and talent management.FindingsThe key indicators of perceived usefulness are productivity, perceived ease of use, adaptability, candidate experience with the adoption of AI, frequency in decision-making in its actual usage and career path of development in the HEIs. These are the most influential items impacting the application of AI in TA.Originality/valueAI has the potential to revolutionize TA in HEIs in the form of enhanced efficiency, improved candidate experience, more objective hiring decisions, talent analytics and risk automation. However, they facilitate resume screening, candidate sourcing, applicant tracking, interviewing and predictive analytics for attrition.
Revealing governance forms: the case of French agrifood platforms
Authors: Guillot Romane , Aubert Magali , Mione Anne 
Published: May 2024

PurposeAgrifood platforms are now part of consumption habits. They have emerged in various forms, and we need to describe this diversity to understand better how platforms manage their relationships with farmers. We aim to understand the governance forms of agrifood platforms and consider whether they comply with the principles of transaction cost economics (TCE).Design/methodology/approachBased on a survey of 103 French platform managers, a two-step cluster analysis and ordered logit regressions were applied to test hypotheses derived from the theory.FindingsThe results enable us to propose a refined typology of eight governance forms for the farmer-platform relationship. These different forms can be classified according to a continuum ranging from “market to hierarchy”, conforming to TCE principles. We define a gradient describing how the platforms manage their relations with the farmers through contractual and relational control. We show that specific assets, behavioural uncertainty, and membership in a platform network are associated with more integrated governance forms.Practical implicationsThe article describes the different forms of platform governance and their relevance to market conditions. This clarification is necessary for farmers to elect the more suitable platform and for platform managers to create a new business or improve its efficiency.Originality/valueThis article is the first to offer a detailed typology of agrifood platform governance. It highlights these governance characteristics and their relationship with transaction attributes.
Ethnic minorities and income inequality: the Albanian community in Italy
Authors: Errico Lucia , Mosca Andrea , Rondinella Sandro 
Published: May 2024

PurposeThis study explores whether ethnic minorities exhibit varying levels of income inequality compared to the host population.Design/methodology/approachThe research leverages a unique immigration event in Italy, specifically the settlement of multiple Albanian groups in southern Italy during the 16th century. This historical occurrence enables an investigation into the role of cultural traits in income inequality, as these groups are situated in the same geographical region and often share borders.FindingsThe results, which remain consistent after undergoing various robustness checks, indicate that Albanian villages, while still preserving their identity and tradition, tend to experience an approximately 2% lower level of income concentration compared to similar Italian municipalities.Originality/valueOur findings aim to provide supporting evidence for future policy considerations regarding the long-term impact of immigration on income inequality.
Demystifying operator 4.0 through an integrative framework
Authors: Nujen Bella Belerivana , Mwesiumo Deodat , Alfnes Erlend , Tomasgard Tore 
Published: May 2024

PurposeDespite increased interest in the Operator 4.0 concept, there has been scarce attention on practice-oriented frameworks that can assist firms in reaping the potential benefits of technologies within the I4.0 framework. The purpose of this paper is to provide an integrative framework on how to engage Operator 4.0 effectively.Design/methodology/approachFollowing meta-synthesis logic and integrative conceptual analysis, this study synthesizes and integrates experimental case studies on Operator 4.0 solutions. The analysis is conducted on 24 cases retrieved from peer-reviewed articles.FindingsThe analysis revealed several factors that determine the type and extent of benefits that can be realized within a human-cyber-physical system. However, enhancing these capabilities is not straightforward, as several factors can inhibit the realization of these capabilities. Firstly, the benefits vary depending on the type of Operator 4.0. Secondly, the interoperability of the introduced I4.0 solution with the existing systems is crucial. Thirdly, the adaptability of the solution to serve multiple purposes is crucial, as it tends to increase technology acceptance.Research limitations/implicationsThe studies reviewed in this research were based on experimental cases, with limited implementation of the solutions they proposed. As such, their implications when implemented on a larger scale are tentative.Originality/valueThe present study has synthesized existing experimental cases and developed an integrative framework to guide the implementation of Operator 4.0. By gathering primary insights from these experiments, we have clarified when and how Operator 4.0 and the required interactions can lead to successful implementation.