bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleolin myocardial-specific knockout exacerbates glucose metabolism disorder in endotoxemia-induced myocardial injury
Authors: Tang Yuting , Yin Leijing , Yuan Ludong , Lin Xiaofang , Jiang Bimei 
Published: May 2024

Background
Sepsis-induced myocardial injury, as one of the important complications of sepsis, can significantly increase the mortality of septic patients. Our previous study found that nucleolin affected mitochondrial function in energy synthesis and had a protective effect on septic cardiomyopathy in mice. During sepsis, glucose metabolism disorders aggravated myocardial injury and had a negative effect on septic patients.

Objectives
We investigated whether nucleolin could regulate glucose metabolism during endotoxemia-induced myocardial injury.

Methods
The study tested whether the nucleolin cardiac-specific knockout in the mice could affect glucose metabolism through untargeted metabolomics, and the results of metabolomics were verified experimentally in H9C2 cells. The ATP content, lactate production, and oxygen consumption rate (OCR) were evaluated.

Results
The metabolomics results suggested that glycolytic products were increased in endotoxemia-induced myocardial injury, and that nucleolin myocardial-specific knockout altered oxidative phosphorylation-related pathways. The experiment data showed that TNF-α combined with LPS stimulation could increase the lactate content and the OCR values by about 25%, and decrease the ATP content by about 25%. However, interference with nucleolin expression could further decrease ATP content and OCR values by about 10–20% and partially increase the lactate level in the presence of TNF-α and LPS. However, nucleolin overexpression had the opposite protective effect, which partially reversed the decrease in ATP content and the increase in lactate level.

Conclusion
Down-regulation of nucleolin can exacerbate glucose metabolism disorders in endotoxemia-induced myocardial injury. Improving glucose metabolism by regulating nucleolin was expected to provide new therapeutic ideas for patients with septic cardiomyopathy.
Folding of a Donor‐Containing Ionene by Intercalation with an Acceptor
Authors: De Swati , Ramakrishnan S. 
Published: November 2010

AbstractCationic ionenes that bear electron‐rich 1,5‐dialkoxynaphthalene (DAN) units within the alkylene segment were allowed to interact with different types of electron‐deficient, acceptor‐containing molecules in an effort to realize intercalation‐induced folding of the ionenes; the collapse of the chains was expected to occur in such a way that the donor and acceptor units become arranged in an alternating fashion. Several acceptor‐bearing molecules were prepared by the derivatization of pyromellitic dianhydride and naphthalene tetracarboxylic dianhydride with two different oligoethylene glycol monomethyl ether monoamines. This yielded acceptor molecules with different water solubility and allowed the examination of solvophobic effects in the folding process. UV/Vis spectroscopic studies were carried out by using a 1:1 mixture of the DAN–ionenes and different acceptor molecules in water/DMSO solvent mixtures. The intensity of the charge‐transfer (CT) band was seen to increase with the water content in the solvent mixture, thereby suggesting that the intercalation is indeed aided by solvophobic effects. The naphthalene diimide (NDI) bearing acceptor molecules consistently formed significantly stronger CT complexes when compared to the pyromellitic diimide (PDI) bearing acceptor molecules, which is a reflection of the stronger π‐stacking tendency of the former. AFM studies of drop‐cast films of different ionene–acceptor combinations revealed that compact folded structures are formed most effectively under conditions in which the strongest CT complex is formed.
Global DNA methylation measurement by HPLC using low amounts of DNA
Authors: Armstrong Kelly M. , Bermingham Emma N. , Bassett Shalome A. , Treloar Bryan P. , Roy Nicole C. , Barnett Matthew PG. 
Published: November 2010

AbstractEpigenetic changes in chromatin structure can influence gene expression without affecting the DNA sequence. The most commonly studied epigenetic modification, DNA methylation, has been implicated in normal tissue development and disease progression, and can be influenced by diet and other environmental factors. Current HPLC methods of determining DNA methylation may require relatively large amounts of DNA (50 μg); as many tissues have low DNA yields, this can be hard to achieve. We isolated DNA from mouse colon and liver in a study investigating post‐natal supplementation with selenium and folic acid. After optimizing the methods to account for lower initial DNA amounts, we digested 3 μg of DNA to deoxynucleotide monophosphates, then purified and quantified it. Samples were analyzed by reversed‐phase HPLC to determine global DNA methylation levels using commercial nucleotide standards. The HPLC column was cooled to 6(C (reducing run time), and detection was at 280 nm (UV). We showed that methylated cytosine can be accurately and reproducibly measured in as little as 3 μg of DNA using this HPLC analysis method (within‐assay CV <2%). We also used this method to detect reduced DNA methylation in liver (P = 0.009) in response to post‐natal supplementation with selenium and folate.
Applying fixed‐priority preemptive scheduling with preemption threshold to asynchronous event handling in the RTSJ
Authors: Kim MinSeong , Wellings Andy 
Published: November 2010

AbstractThe primary goal of asynchronous event handlers (handlers for short) in the Real‐Time Specification for Java (RTSJ) is to have a lightweight concurrency mechanism. The emphasis here is on ‘lightweightness’ which should be achieved by having fewer real‐time threads (servers for short) to execute more handlers. However, it is generally unclear how to efficiently map handlers to servers. In this paper we first define the worst case scenario that demands the least upper bound of servers for self‐suspending and non‐self‐suspending handlers. Based on the worst case scenarios, it is proved that the number of servers required to execute a given number of non‐self‐suspending handlers depends on the number of priority levels, and not on the number of handlers. It is also shown that each self‐suspending handler is required to have its own server to prevent unbounded priority inversion. Based on these results we also propose to adapt the notion of preemption threshold to further minimize the number of priority levels in the system. We have extended the non‐self‐suspending asynchronous event handling (AEH) implementation to support the fixed‐priority scheduling with preemption threshold on an existing RTSJ implementation to show that the number of servers can be further decreased while feasibly scheduling a set of non‐self‐suspending handlers, regardless of the number of the handlers or the priority levels in the system. Copyright © 2010 John Wiley & Sons, Ltd.
Structural Investigation of Coordination Polymers Constructed from a Conformational Bis‐triazole Ligand and V‐Shaped Bridging Carboxylate Anions: Hydrothermal Syntheses, Crystal Structures, and Property Studies
Authors: Ren Chen , Liu Ping , Wang Yao‐Yu , Huang Wen‐Huan , Shi Qi‐Zhen 
Published: November 2010

AbstractThe conformational bis‐triazole ligand 4,4′‐bis[(1,2,4‐triazol‐1‐yl)methyl]biphenyl (btmb, 1), was successfully synthesized, and seven new coordination polymers, namely {M(oba)(btmb)}n [M = Cd2+ (2), Co2+ (3), Ni2+ (4), and Zn2+ (5)], {M(sdba)(btmb)}n [M = Co2+ (6) and Cd2+ (7)], and {[Cu(H2odpa)(btmb)(H2O)]·2H2O}n (8), (H2oba = 4,4′‐oxydibenzoic acid, H2sdba = 4,4′‐sulfonyldibenzoic acid, and H4odpa = 4,4′‐oxydiphthalic acid), have been synthesized by utilizing the btmb ligand and the polymers have been characterized in detail. Complexes 2–4 are isostructural exhibiting 2D‐layer structures and can be described as (3,4)‐connected networks with (42.63.8)(42.6) topological notation. Complex 5 shows 2D layers with left‐ and right‐handed helical chains and has a (3,4)‐connected net with (4.52)(4.53.72) topology. Complexes 6 and 7 are isostructural and display 2D undulating networks with (4,4) sheets that are further packed through van der Waals interactions to generate a 3D supramolecular structure. Complex 8 also represents a 2D network with a 44‐sql net, which is extended into a 3D supramolecular framework by hydrogen‐bonding interactions. The structural differences of these complexes demonstrate that the conformation of the btmb ligand and flexible aromatic polycarboxylate anions play critical roles in the formation of the resulting frameworks. Furthermore, their thermal properties have been studied by thermogravimetric analysis (TGA) and the TGA results reveal that the triazole‐based frameworks have a high thermal stability, which suggests they are good candidates for the construction of more stable complexes. The photoluminescent properties of complexes 2, 5, and 7 have also been studied.
In vitro assessment of osteoblast and macrophage mobility in presence of β‐TCP particles by videomicroscopy
Authors: Beuvelot Johanne , Pascaretti‐Grizon Florence , Filmon Robert , Moreau Marie‐Fraçoise , Baslé Michel F. , Chappard Daniel 
Published: November 2010

Abstractβ‐TCP is widely used to repair bone defects due to its good biocompatibility, macroporosity (favoring bone ingrowth) and bioresorbability. However, cell interactions with the biomaterial at the first times of implantation remain largely unknown. We have observed cell behaviors in direct contact with β‐TCP particles using long‐term culture under videomicroscopy. Osteoblastlike cells (SaOs‐2) and macrophages (J774.2 and mouse peritoneal macrophages) were cultured in the presence of β‐TCP particles. For each experiment, images from 20 independent fields were acquired and stored every 15 min during 8 days. At the end of the culture, they were combined to generate time lapse videos; coverslips were fixed and observed by scanning electron microscopy (SEM). SaOs‐2 proliferation was determined by counting cells on six different and independent fields at days 1, 3, and 6. Videos showed the capacity of cells to displace the particles. Dynamic follow‐up showed active proliferation of SaOs‐2 occurring in the direction of the particles. J774.2 and peritoneal macrophages did not proliferate but came in direct contact with the particles and actively eroded them. SEM showed that cells were stretched and fixed onto the surface and seemed to climb from the coverslip to the particles. The long‐term culture under videomicroscopy allowed a better understanding of the colonization process of β‐TCP particles by osteoblastlike cells and macrophages. Data obtained from long‐term videomicroscopy are in agreement with in vivo observations confirming the interest of β‐TCP to promote osteogenesis. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Computational study on the reactions of H2O2 on TiO2 anatase (101) and rutile (110) surfaces
Authors: Huang Wen‐Fei , Raghunath P. , Lin M. C. 
Published: November 2010

AbstractThis study investigates the adsorption and reactions of H2O2 on TiO2 anatase (101) and rutile (110) surfaces by first‐principles calculations based on the density functional theory in conjunction with the projected augmented wave approach, using PW91, PBE, and revPBE functionals. Adsorption mechanisms of H2O2 and its fragments on both surfaces are analyzed. It is found that H2O2, H2O, and HO preferentially adsorb at the Ti5c site, meanwhile HOO, O, and H preferentially adsorb at the (O2c)(Ti5c), (Ti5c)2, and O2c sites, respectively. Potential energy profiles of the adsorption processes on both surfaces have been constructed using the nudged elastic band method. The two restructured surfaces, the 1/3 ML oxygen covered TiO2 and the hydroxylated TiO2, are produced with the H2O2 dehydration and deoxidation, respectively. The formation of main products, H2O(g) and the 1/3 ML oxygen covered TiO2 surface, is exothermic by 2.8 and 5.0 kcal/mol, requiring energy barriers of 0.8 and 1.1 kcal/mol on the rutile (110) and anatase (101) surface, respectively. The rate constants for the H2O2 dehydration processes have been predicted to be 6.65 × 10−27 T4.38 exp(−0.14 kcal mol−1/RT) and 3.18 × 10−23 T5.60 exp(−2.92 kcal mol−1/RT) respectively, in units of cm3 molecule−1 s−1. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011.
An ARDL model of unrecorded and recorded economies in Turkey
Authors: Dell'Anno Roberto , Halicioglu Ferda 
Published: November 2010

PurposeThe goal of this paper is twofold: to estimate the unrecorded economy (UE) of Turkey over the period 1987‐2007 using a revised version of the currency demand approach, and to analyze the relationship between the UE and recorded GDP.Design/methodology/approachThe paper proposes to measure the UE using the autoregressive distributed lag (ARDL) approach to cointegration analysis. Toda‐Yamamoto causality tests are also conducted to identify the relationship between unrecorded and recorded GDP.FindingsThis research provides fresh evidence of the size of the UE relative to the recorded GDP in Turkey, which ranges from 10.7 percent to 18.9 percent over the estimation period. Moreover, empirical evidence concretely suggests that causality runs from the recorded GDP to the UE. However, there exists a mild reverse causality.Research limitations/implicationsMeasures of the UE, and particularly those based on monetary approaches, have been criticized on several counts, including their lack of robustness and weak theoretical foundations (e.g. the velocity of money in the recorded economy and in the UE is the same).Practical implicationsThis analysis suggests that the UE is pro‐cyclical with respect to the recorded GDP. It suggests that the phenomenon of the UE is more dangerous when the economy is in an expensive phase. Hence, during a positive business cycle, it is clearly desirable for the government that the anti‐UE controls should be more effective.Originality/valueThe ARDL approach to estimating the size of the UE eliminates the criticism of the previous currency demand estimations, which were based on partial adjustment models. Therefore, the paper's econometric selected cointegration methodology and causality test is an improvement over the existing studies.
Rice bran adhesive modified with potassium permanganate and poly(vinyl alcohol)
Authors: Wang W. , Zhang X. , Li F. , Qi C. 
Published: November 2010

PurposeThe purpose of this paper is to develop an environmental adhesive from defatted rice bran (RB) to increase its economic value and provide environmentally friendly products for the wood industry.Design/methodology/approachThe powder was mixed with water in a flask. After pH adjustment, potassium permanganate (KMnO4) solution was added. Then the mixture was heated in a shaking water bath. After maintaining the mixture at 70°C for 1 h, poly(vinyl alcohol) (PVA) powder was added to the slurry and heated at the same temperature for a further 0.5 h. The adhesive thus prepared was used to bond veneers to evaluate its bonding strength and analysed by IR. The optimised RB adhesive was mixed with phenol formaldehyde resin in a ratio of 8:8 to bond straw particleboard. Silane was used to improve the properties of the straw particleboard.FindingsAs an economically renewable resource, RB has potential use in the preparation of adhesives. When RB powder was treated with KMnO4 and PVA at pH 6, the resulting adhesive was able to produce a bonding strength of 1.29 MPa. Silane was found to be a useful coupling agent in the bonding of straw particleboard. Once the board had been bonded with the mixed adhesive, it was found to have a bending strength of 11.9 MPa and a modulus of elasticity of 2.2 GPa.Research limitations/implicationsChemical change was not obvious during RB modification, which led to a low water resistance. Further research is needed to modify the method.Practical implicationsRB adhesive was dark in colour, which limits its use for light‐coloured wood. Because of its lower water resistance, this adhesive has to be mixed with other synthetic resins to meet requirements.Originality/valueA new adhesive product was developed for the wood industry from economic RB. This will contribute to environmentally friendly wood products and the effective utilisation of agricultural residues.
Treatment of focal idiopathic hyperhidrosis with Botulinum Toxin Type A: clinical predictive factors of relapse‐free survival
Authors: Campanati A. , Sandroni L. , Gesuita R. , Giuliano A. , Giuliodori K. , Marconi B. , Ganzetti G. , Offidani A. 
Published: November 2010

AbstractBackground  No material about the identification of predictive clinical factors of therapeutic response to Botulinum Toxin Type A (BTX‐A) in focal idiopathic hyperhidrosis has been found.Objective  To evaluate if age, sex, extension rate of hyperhidrotic area, localization, disease‐related impairment of life quality, number of previous local, non‐invasive treatments different from BTX‐A, and duration of disease, may affect the relapse‐free survival (RFS) after a BTX‐A treatment in palmar and axillary focal idiopathic hyperhidrosis.Methods  Forty‐one patients suffering from palmar hyperhidrosis, and 38 patients suffering from axillary hyperhidrosis received intradermal injections of BTX‐A. All patients were clinically screened before and after treatment; they were followed for 15 months after it, according to Hyperhidrosis Disease Severity Scale (HDSS), Minor’s test, and DLQI test, to state disease severity, and disease‐related impairment of quality of life.Results  The duration of therapeutic effect of BTX‐A is not significantly influenced by age (P = 0.783), sex (P = 0.762), extension of hyperhidrotic area (P = 0.770), site of involvement (P = 0.402), disease‐induced impairment of life quality (P = 0.745), number of previous therapies (P = 0.730), or site of involvement (P = 0.402). In palmar idiopathic hyperhidrosis, patients with a longer disease history show a shorter duration of RFS after a treatment with BTX‐A (P = 0.01).Conclusions  Patients suffering from palmar hyperhidrosis have a longer lasting disease, and a length of disease more than 20 years in these patients influences the RFS after BTX‐A treatment.
The polymorphism of catalase T/C codon 389 in exon 9 and vitiligo susceptibility: a meta‐analysis
Authors: Lv Y.J. , Liao W.J. , Luan Q. , Wang H. , Wang L. , Li Q. 
Published: November 2010

AbstractBackground  The exact aetiology of vitiligo has not yet been established. Oxidative stress is involved in the pathophysiology of vitiligo. It has been described that some polymorphisms in the catalase (CAT) gene may affect the risk of vitiligo. However, the results were inconsistent.Objective  We performed a meta‐analysis of the published studies to derive a more precise estimate of the association between CAT T/C at codon 389 in exon 9 polymorphisms and vitiligo risk.Methods  The PubMed, Medline, Cochrane Library, China National Knowledge Infrastructure (CNKI) databases were searched to identify relevant published studies.Results  Four case–control studies (cases, 645; controls, 689) that investigated the association between C/T polymorphisms of CAT exon 9 and the risk of vitiligo were retrieved and analysed. Our findings suggested a significant association between the CAT T/C exon 9 polymorphism and vitiligo risk (CT + TT vs. CC pooled odds ratio, 1.43; 95% confidence interval, 1.14–1.80; P = 0 .002).Conclusion  We found a significant correlation between the CAT T/C exon 9 polymorphism and the risk of vitiligo.
A Randomized Controlled Trial of the Effects of Vitamin D on Muscle Strength and Mobility in Older Women with Vitamin D Insufficiency
Authors: Zhu Kun , Austin Nicole , Devine Amanda , Bruce David , Prince Richard L. 
Published: November 2010

OBJECTIVES: To evaluate the effects of vitamin D treatment on muscle strength and mobility in older women with vitamin D insufficiency.DESIGN: One‐year population‐based, double‐blind, randomized, controlled trial.SETTING: Perth, Australia (latitude 32°S).PARTICIPANTS: Three hundred two community‐dwelling ambulant elderly women aged 70 to 90 with a serum 25‐hydroxyvitamin D (25(OH)D) concentration less than 24 ng/mL.INTERVENTION: Vitamin D2 1,000 IU/d or identical placebo; calcium citrate (1 g calcium/d) in both groups.MEASUREMENTS: Lower limb muscle strength and mobility as assessed using the Timed Up and Go Test (TUAG).RESULTS: At baseline, mean±standard deviation serum 25(OH)D was 17.7±4.2 ng/mL; this increased to 24.0±5.6 ng/mL in the vitamin D group after 1 year but remained the same in the placebo group. For hip extensor and adductor strength and TUAG, but not for other muscle groups, a significant interaction between treatment group and baseline values was noted. In those with baseline values in the lowest tertile, vitamin D improved muscle strength and TUAG more than calcium alone (mean (standard error): hip extensors 22.6% (9.5%); hip adductors 13.5% (6.7%), TUAG 17.5% (7.6%), P<.05). Baseline 25(OH)D levels did not influence patient response to supplementation.CONCLUSION: Vitamin D therapy was observed to increase muscle function in those who were the weakest and slowest at baseline. Vitamin D should be given to people with insufficiency or deficiency to improve muscle strength and mobility.
Functional magnetic resonance imaging studies of acupuncture mechanisms: a critique
Authors: Beissner Florian 
Published: November 2010

AbstractFunctional magnetic resonance imaging has been used extensively to study the underlying mechanisms of acupuncture. More than 80 studies and a dozen review articles have been published on this topic in the past 10 years. Emphasising the statistical quality of the studies, this critical review evaluates the results obtained so far by studies that have used a hypothesis‐driven approach. A minimum sample size of 12 subjects, the application of random effects (or mixed effects) analyses, and a threshold corrected for multiple comparisons were defined as inclusion criteria for a meta‐analysis of the reported cortical activations. Fourteen out of 71 studies met the inclusion criteria. The majority of these studies reported activations in cortical areas relevant to the processing of somatosensory, motor, or pain signals, as well as areas related to the special senses. From this type of study, it is so far impossible to say if cortical activations under acupuncture are part of an underlying mechanism, or if they simply reflect the brain's processing of the somatosensory or pain stimulus from the acupuncture needle stimulation. In the future, the application of data‐driven methods may lead to a more comprehensive understanding of neuronally mediated acupuncture effects.
The Limits of the Explanatory Power of Developmentalism
Authors: Sobel David 
Published: November 2010

AbstractRichard Kraut's neo-Aristotelian account of well-being, Developmentalism, aspires to explain not only which things are good for us but why those things are good for us. The key move in attempting to make good on this second aspiration involves his claim that our ordinary intuitions about what is good for a person can be successfully explained and systematized by the idea that what benefi ts a living thing develops properly that living thing's potentialities, capacities, and faculties. I argue that Kraut's understanding of such proper development plays no serious constraining role in shaping the details of the account. If this is correct, Developmentalism lacks the potential to explain or vindicate the intuitions about what is good for us that it champions. In effect, Kraut offers us a list of things that he claims benefits a person, but he lacks a theory of what those things have in common such that they benefit him.