bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated serum IGF-1 levels synergize PTH action on the skeleton only when the tissue IGF-1 axis is intact
Authors: Elis Sebastien , Courtland Hayden-William , Wu Yingjie , Fritton J Christopher , Sun Hui , Rosen Clifford J , Yakar Shoshana 
Published: April 2010

Abstract
There is growing evidence that insulin-like growth factor 1 (IGF-1) and parathyroid hormone (PTH) have synergistic actions on bone and that part of the anabolic effects of PTH is mediated by local production of IGF-1. In this study we analyzed the skeletal response to PTH in mouse models with manipulated endocrine or autocrine/paracrine IGF-1. We used mice carrying a hepatic IGF-1 transgene (HIT), which results in a threefold increase in serum IGF-1 levels and normal tissue IGF-1 expression, and Igf1 null mice with blunted IGF-1 expression in tissues but threefold increases in serum IGF-1 levels (KO-HIT). Evaluation of skeletal growth showed that elevations in serum IGF-1 in mice with Igf1 gene ablation in all tissues except the liver (KO-HIT) resulted in a restoration of skeletal morphology and mechanical properties by adulthood. Intermittent PTH treatment of adult HIT mice resulted in increases in serum osteocalcin levels, femoral total cross-sectional area, cortical bone area and cortical bone thickness, as well as bone mechanical properties. We found that the skeletal response of HIT mice to PTH was significantly higher than that of control mice, suggesting synergy between IGF-1 and PTH on bone. In sharp contrast, although PTH-treated KO-HIT mice demonstrated an anabolic response in cortical and trabecular bone compartments compared with vehicle-treated KO-HIT mice, their response was identical to that of PTH-treated control mice. We conclude that (1) in the presence of elevated serum IGF-1 levels, PTH can exert an anabolic response in bone even in the total absence of tissue IGF-1, and (2) elevations in serum IGF-1 levels synergize PTH action on bone only if the tissue IGF-1 axis is intact. Thus enhancement of PTH anabolic actions depends on tissue IGF-1. © 2010 American Society for Bone and Mineral Research.
Systemic effects of ulna loading in male rats during functional adaptation
Authors: Sample Susannah J , Collins Ryan J , Wilson Aliya P , Racette Molly A , Behan Mary , Markel Mark D , Kalscheur Vicki L , Hao Zhengling , Muir Peter 
Published: April 2010

Abstract
Functional skeletal adaptation is thought to be a local phenomenon controlled by osteoctyes. However, the nervous system also may have regulatory effects on adaptation. The aim of this study was to determine the effects of loading of a single bone on adaptation of other appendicular long bones and whether these responses were neuronally regulated. Young male Sprague-Dawley rats were used. The right ulna was loaded to induce a modeling response. In other rats, a second regimen was used to induce bone fatigue with a mixed modeling/remodeling response; a proportion of rats from each group received brachial plexus anesthesia to induce temporary neuronal blocking during bone loading. Sham groups were included. Left and right long bones (ulna, humerus, tibia, and femur) from each rat were examined histologically 10 days after loading. In fatigue- and sham-loaded animals, blood plasma concentrations of TNF-α, RANKL, OPG, and TRAP5b were determined. We found that loading the right ulna induced an increase in bone formation in distant long bones that were not loaded and that this effect was neuronally regulated. Distant effects were most evident in the rats that received loading without bone fatigue. In the fatigue-loaded animals, neuronal blocking induced a significant decrease in plasma TRAP5b at 10 days. Histologically, bone resorption was increased in both loaded and contralateral ulnas in fatigue-loaded rats and was not significantly blocked by brachial plexus anesthesia. In young, growing male rats we conclude that ulna loading induced increased bone formation in multiple bones. Systemic adaptation effects were, at least in part, neuronally regulated. © 2010 American Society for Bone and Mineral Research.
Bradykinin‐induced cell migration and COX‐2 production mediated by the bradykinin B1 receptor in glioma cells
Authors: Lu Dah‐Yuu , Leung Yuk‐Man , Huang Ssu‐Ming , Wong Kar‐Lok 
Published: April 2010

AbstractBradykinin is produced and acts at the site of injury and inflammation. Recent reports have also shown that bradykinin selectively modulates blood–tumor barrier permeability. However, the molecular mechanisms and pathologic roles underlying bradykinin‐induced glioma migration remain unclear. Glioma is the most common primary adult brain tumor, with a poor prognosis because of the ease with which tumor cells spread to other regions of the brain. In this study, we found that bradykinin increases the cell migration and expression of cyclo‐oxygenase‐2 (COX‐2) in glioma cells. Bradykinin‐mediated migration was attenuated by the selective COX‐2 inhibitor NS‐398. Moreover, increased motility of glioma cells and expression of COX‐2 were mimicked by a bradykinin B1 receptor (B1R) agonist and markedly inhibited by a B1R antagonist. Bradykinin‐mediated migration was attenuated by phosphoinositide 3‐kinase (PI‐3 kinase)/AKT inhibitors LY 294002 and wortmannin. Bradykinin stimulation also increased the phosphorylation of the p85 subunit of PI‐3 kinase and serine 473 of AKT. Treatment of bradykinin with AP‐1 inhibitors Tanshinone IIA and curcumin also reduced COX‐2 expression and glioma cell migration. Moreover, treatment of bradykinin also induced phosphorylation of c‐Jun in glioma cells. AP‐1 promoter analysis in the luciferase reporter construct showed that bradykinin increased AP‐1 transcription activity and was inhibited by LY 294002 and wortmannin. One mechanism underlying bradykinin‐directed migration is transcriptional up‐regulation of COX‐2 and activation of the B1R receptor, PI‐3 kinase, AKT, c‐Jun, and AP‐1 pathways. J. Cell. Biochem. 110: 141–150, 2010. © 2010 Wiley‐Liss, Inc.
A conserved post‐transcriptional BMP2 switch in lung cells
Authors: Jiang Shan , Fritz David T. , Rogers Melissa B. 
Published: April 2010

AbstractAn ultra‐conserved sequence in the bone morphogenetic protein 2 (BMP2) 3′ untranslated region (UTR) markedly represses BMP2 expression in non‐transformed lung cells. In contrast, the ultra‐conserved sequence stimulates BMP2 expression in transformed lung cells. The ultra‐conserved sequence functions as a post‐transcriptional cis‐regulatory switch. A common single‐nucleotide polymorphism (SNP, rs15705, +A1123C), which has been shown to influence human morphology, disrupts a conserved element within the ultra‐conserved sequence and altered reporter gene activity in non‐transformed lung cells. This polymorphism changed the affinity of the BMP2 RNA for several proteins including nucleolin, which has an increased affinity for the C allele. Elevated BMP2 synthesis is associated with increased malignancy in mouse models of lung cancer and poor lung cancer patient prognosis. Understanding the cis‐ and trans‐regulatory factors that control BMP2 synthesis is relevant to the initiation or progression of pathologies associated with abnormal BMP2 levels. J. Cell. Biochem. 110: 509–521, 2010. © 2010 Wiley‐Liss, Inc.
PR‐Set7‐mediated monomethylation of histone H4 lysine 20 at specific genomic regions induces transcriptional repression
Authors: Congdon Lauren M. , Houston Sabrina I. , Veerappan Chendhore S. , Spektor Tanya M. , Rice Judd C. 
Published: April 2010

AbstractIncreasing evidence indicates that the post‐translational modifications of the histone proteins play critical roles in all eukaryotic DNA‐templated processes. To gain further biological insights into two of these modifications, the mono‐ and trimethylation of histone H4 lysine 20 (H4K20me1 and H4K20me3), ChIP‐chip experiments were performed to identify the precise genomic regions on human chromosomes 21 and 22 occupied by these two modifications. Detailed analysis revealed that H4K20me1 was preferentially enriched within specific genes; most significantly between the first ∼5% and 20% of gene bodies. In contrast, H4K20me3 was preferentially targeted to repetitive elements. Among genes enriched in H4K20me3, the modification was typically targeted to a small region ∼1 kb upstream of transcription start. Our collective findings strongly suggest that H4K20me1 and H4K20me3 are both physically and functionally distinct. We next sought to determine the role of H4K20me1 in transcription since this has been controversial. Following the reduction of PR‐Set7/Set8/KMT5a and H4K20me1 in cells by RNAi, all H4K20me1‐associated genes analyzed displayed an ∼2‐fold increase in gene expression; H4K20me3‐associated genes displayed no changes. Similar results were obtained using a catalytically dead dominant negative PR‐Set7 indicating that H4K20me1, itself, is essential for the selective transcriptional repression of H4K20me1‐associated genes. Furthermore, we determined that the H4K20me1‐associated DNA sequences were sufficient to nucleate H4K20me1 and induce repression in vivo. Our findings reveal the molecular mechanisms of a mammalian transcriptional repressive pathway whereby the DNA sequences within specific gene bodies are sufficient to nucleate the monomethylation of H4K20 which, in turn, reduces gene expression by half. J. Cell. Biochem. 110: 609–619, 2010. © 2010 Wiley‐Liss, Inc.
Transcriptional regulation of bone sialoprotein gene by Porphyromonas gingivalis lipopolysaccharide
Authors: Li Xinyue , Kato Naoko , Mezawa Masaru , Li Zhengyang , Wang Zhitao , Yang Li , Sasaki Yoko , Kaneko Takashi , Takai Hideki , Yoshimura Atsutoshi , Ogata Yorimasa 
Published: April 2010

AbstractLipopolysaccharide (LPS) is a major mediator of inflammatory response. Periodontopathic bacterium Porphyromonas gingivalis LPS has quite different character from Escherichia coli LPS. E. coli LPS is agonist for Toll‐like receptor 4 (TLR4), whereas P. gingivalis LPS worked as antagonist for TLR4. Bone sialoprotein (BSP) is an early marker of osteoblast differentiation. To investigate the effects of P. gingivalis LPS on BSP transcription, we used rat osteoblast‐like ROS17/2.8 cells. BSP mRNA levels were decreased by 0.1 µg/ml and increased by 0.01 µg/ml P. gingivalis LPS at 12 h. Results of luciferase assays showed that 0.1 µg/ml decreased and 0.01 µg/ml P. gingivalis LPS increased BSP transcription in −116 to +60 BSP construct. The effects of P. gingivalis LPS were abrogated by double mutations in cAMP response element (CRE) and FGF2 response element (FRE). Tyrosine kinase inhibitor herbimycin A, ERK1/2 inhibitor and antioxidant N‐acetylcystein inhibited effects of P. gingivalis LPS. Protein kinase A inhibitor and PI3‐kinase/Akt inhibitor only abolished the effect of 0.01 µg/ml P. gingivalis LPS. Furthermore, 0.1 µg/ml LPS decreased the CRE– and FRE–protein complexes formation, whereas 0.01 µg/ml P. gingivalis LPS increased the nuclear protein binding to CRE and FRE. ChIP assays revealed increased binding of CREB1, JunD, Fra2, Runx2, Dlx5, and Smad1 to a chromatin fragment containing the CRE and FRE by 0.01 µg/ml P. gingivalis LPS. These studies therefore indicated that 0.1 µg/ml suppressed, and 0.01 µg/ml P. gingivalis LPS increased BSP gene transcription mediated through CRE and FRE elements in the rat BSP gene promoter. J. Cell. Biochem. 110: 823–833, 2010. © 2010 Wiley‐Liss, Inc.
Development and validation of brief content scales for the psychological screening inventory‐2
Authors: Lanyon Richard I. 
Published: April 2010

AbstractThis article describes the development of 21 brief content (BC) scales to supplement the existing scales of the Psychological Screening Inventory‐2 (PSI‐2), and three validity studies to support their use for both the PSI‐2 and the original PSI. The BC scales comprise groups of four or more items that are statistically homogeneous in content and are replicated across three data sets: PSI and PSI‐2 normative data and a PSI‐based group of forensic respondents. Concurrent validity was shown in correlations with the following: (a) the Minnesota Multiphasic Personality Inventory‐2 (MMPI‐2) basic clinical scales, Content scales, PSY‐5 scales, and Restructured Clinical (RC) scales; (b) family/friend observer ratings; and (c) therapist ratings in an outpatient substance abuse treatment program. Consistent with the purpose of the PSI/PSI‐2, the BC scales are intended to provide additional screening information and not a comprehensive representation of psychopathology. © 2010 Wiley Periodicals, Inc. J Clin Psychol: 66:1–13, 2010.
Coca‐Cola Enterprises invests in on‐boarding at the front lines to benefit the bottom line
Authors: Fritz Kelly , Kaestner Mark , Bergmann Marshall 
Published: April 2010

AbstractThe world's largest bottler of nonalcoholic beverages has made a training investment in new customer‐facing employees as a strategy for reducing turnover, improving productivity, and increasing employee engagement. The CCE Pathway, a structured program of daily self‐study, on‐the‐job learning, peer coaching, reflection, and weekly manager assessments, accelerates learning so that new frontline employees know everything they need to keep customers happy. The authors explain the two‐to‐four‐week program's structure for experiential learning tailored to each position's requirements. Learning objectives for each day focus questions, conversations, and feedback on the knowledge and actions important for individual success and business results. Ongoing coaching and evaluation ensure that learning translates into performance. © 2010 Wiley Periodicals, Inc.
How a leading medical lab accrediting organization has achieved breakthrough improvement with transformational leadership
Authors: Roberts Tammy , Hacker Stephen , Beigel Doug 
Published: April 2010

AbstractCOLA, a leading clinical laboratory accreditation organization, faced a rapidly contracting market and worsening financial performance but was paralyzed by outmoded systems and a vision and mind‐set hamstrung by attachments to past success. In an eight‐year journey, leaders and staff learned to create transformative change in themselves and on key organizational fronts, subsequently generating breakthrough improvements in performance that have set COLA on a new path to success. The authors discuss the key cornerstones of transformational change; a model of transformational leadership; and the transformation cycle COLA used to integrate these with strategic/business planning, execution, and performance monitoring. They also describe COLA's key initiatives—including governance, leadership and culture, performance measurement, sales and marketing, research and development process, and IT—and present evidence of a robust business and organizational transformation at COLA. © 2010 Wiley Periodicals, Inc.
Does the use of fibrin glue prevent seroma formation after axillary lymphadenectomy for breast cancer? A prospective randomized trial in 159 patients
Authors: Cipolla Calogero , Fricano Salvatore , Vieni Salvatore , Graceffa Giuseppa , Licari Gaspare , Torcivia Adriana , Latteri Mario A. 
Published: April 2010

AbstractBackgroundSeroma formation frequently occurs in patients who have undergone axillary lymphadenectomy. The aim of the study was to evaluate the effect of fibrin glue in the prevention of seroma formation after axillary lymphadenectomy.Materials and MethodsHundred fifty‐nine breast cancer patients about to undergo quadrantectomy or mastectomy plus axillary lymphadenectomy were enrolled in the study and randomized into two groups. Fibrin glue spray applied to the axillary fossa plus placement of closed suction drainage were used in 80 patients (group A); placement of closed suction drainage was only used in 79 patients (group B).ResultsGroup A patients showed a slight advantage with regard to the mean duration of axillary drainage placement (4.5 ± 1.3 days in group A vs. 5.1 ± 1.6 days in group B) and number of seroma aspirations (6.3 ± 1.1 in group A vs. 6.7 ± 1.2 in group B). No statistically significant differences were observed between the two groups of patients regarding the mean volume of total axillary drainage and of total seroma volume.ConclusionsThe use of fibrin glue does not prevent seroma formation and does not reduce seroma magnitude and duration. The costs of the product involved do not justify its routine use in patients undergoing axillary dissection. J. Surg. Oncol. 2010; 101:600–603. © 2010 Wiley‐Liss, Inc.
Gastric perforation following cytoreductive surgery with perioperative intraperitoneal chemotherapy
Authors: Zappa Luis , Savady Renaldo , Sugarbaker Paul H. 
Published: April 2010

AbstractObjectiveGastrointestinal tract perforation after cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) has an incidence of 6%. The most common site is the small bowel. The trauma of CRS and delay in wound healing due to HIPEC has been thought to cause this complication.MethodsFrom our database of 1,251 patients we recorded the treatments that resulted in a stomach perforation, the clinical manifestations, and the final outcome. We formulated a pathophysiology for the development of this postoperative complication.ResultsFour patients had postoperative gastrointestinal perforation limited to the wall of the stomach (incidence 0.3%). All patients underwent greater omentectomy with ligation of the gastroepiploic vessels on the surface of the greater curvature, received HIPEC and early perioperative intraperitoneal chemotherapy. All perforations occurred along the greater curvature of the stomach. Successful management of the perforation was by suture plication of the gastric defect.ConclusionPerforation of the stomach following CRS and HIPEC likely results from vascular compromise, delay in wound healing from chemotherapy, seromuscular tears related to traction on the stomach wall and point pressure on the greater curvature from a long‐term indwelling nasogastric tube. Reperitonealization of the greater curvature, if seromuscular tears occur, may help prevent this complication. J. Surg. Oncol. 2010; 101:634–636. © 2010 Wiley‐Liss, Inc.
Adenoma incidence after resection of sporadic colorectal cancer with microsatellite instability
Authors: Kang Ki Joo , Sinn Dong Hyun , Park Sung Hyun , Kim Jin Yong , Chang Dong Kyung , Son Hee Jung , Rhee Poong‐Lyul , Kim Jae J. , Rhee Jong Chul , Chun Ho‐Kyung , Kim Young‐Ho 
Published: April 2010

AbstractBackground and ObjectivesMicrosatellite instability (MSI) tumors that develop via the mismatch repair (MMR) gene pathway show rapid growth. It is unknown if patients with sporadic colorectal cancer (CRC) with MSI would benefit from a shorter interval between colonoscopies. The purpose of this study is to determine if there is a difference in adenoma incidence based on the presence of MSI in patients with sporadic CRC.MethodsWe retrospectively reviewed the incidence of a recurring adenoma at the 1‐ and 3‐year surveillance colonoscopies in 426 patients who had sporadic CRC after surgeryResultsThe number of high MSI (MSI‐H), low MSI (MSI‐L), and microsatellite stable (MSS) tumors were 38 (9%), 27 (6%), and 361 (85%), respectively. After 1 year, the incidence of adenoma (13% vs. 16% for MSI‐L + MSS vs. MSI‐H, P = 0.61) or advanced adenoma (3% vs. 8% for MSI‐L + MSS vs. MSI‐H, P = 0.14) did not differ based on MSI status. The incidence of adenoma or advanced adenoma also did not differ based on MSI status at the 3‐year surveillance colonoscopy.ConclusionsOur study does not show recommendations for a shorter interval for subsequent colonoscopies in patients with sporadic CRC with MSI‐H. Further studies on a large scale are needed. J. Surg. Oncol. 2010; 101:577–581. © 2010 Wiley‐Liss, Inc.
Assessment of open versus laparoscopy‐assisted gastrectomy in lymph node‐positive early gastric cancer: A retrospective cohort analysis
Authors: An Ji Yeong , Heo Geon‐Ung , Cheong Jae‐Ho , Hyung Woo Jin , Choi Seung Ho , Noh Sung Hoon 
Published: April 2010

AbstractBackgroundLaparoscopy‐assisted gastrectomy (LAG) is still limited for early gastric cancer (EGC) with low possibility of lymph node (LN) metastasis, due to the concern for incomplete LN dissection and controversial long‐term outcomes. We assessed oncological outcomes of laparoscopy‐assisted versus open gastrectomy (OG) for patients with LN positive EGC.MethodsBetween 2003 and 2007, 204 patients underwent surgery for LN positive EGC. We evaluated adequacy of LN dissection and early and long‐term outcomes after OG (n = 162) and LAG (n = 42).ResultsOperative time was longer but hospital stay was shorter for LAG than OG. Postoperative complications occurred in 14 patients (8.6%) after OG and 1 patient (2.4%) after LAG (P = 0.316). Mean number of retrieved LNs and number of retrieved and metastatic LNs for each station did not differ between the two groups. During median 35 months of follow‐up, 14 patients (8.6%) developed recurrence after OG, compared with 4 patients (9.5%) after LAG (P = 0.769). Overall 5‐year disease‐free survival was 89.9% and 89.7% after OG and LAG. Status of LN metastasis was the only independent prognostic factor for disease‐free survival.ConclusionsLAG is an oncologically safe procedure even for LN positive EGC. Adequate LN dissection and comparable long‐term outcomes to OG can be achieved by LAG. J. Surg. Oncol. J. Surg. Oncol. 2010;102:77–81. © 2010 Wiley‐Liss, Inc.
Radiofrequency ablation of colorectal liver metastases induces an inflammatory response in distant hepatic metastases but not in local accelerated outgrowth
Authors: Nijkamp Maarten W. , Borren Alie , Govaert Klaas M. , Hoogwater Frederik J.H. , Molenaar I. Quintus , van Diest Paul J. , Kranenburg Onno , Rinkes Inne H.M. Borel 
Published: April 2010

AbstractBackgroundRecently, we have shown in a murine model that radiofrequency ablation (RFA) induces accelerated outgrowth of colorectal micrometastases in the transition zone (TZ) surrounding the ablated lesion. Conversely, RFA also induces an anti‐tumor T‐cell response that may limit tumor growth at distant sites. Here we have evaluated whether an altered density of inflammatory cells could be observed in the perinecrotic (TZ) metastases compared to hepatic metastases in the distant reference zone (RZ).MethodsRFA‐treated tumor‐bearing mice (n = 10) were sacrificed. The inflammatory cell density (neutrophils, macrophages, CD4+ T‐cells, and CD8+ T‐cells) of tumors in the TZ (TZ tumors) was compared to that in tumors in the RZ (RZ tumors). Sham‐operated, tumor‐bearing mice (n = 10) were analyzed simultaneously as controls (sham‐treated tumors).ResultsIn RFA‐treated, tumor‐bearing mice RZ tumors contained a significantly higher density of neutrophils and CD4+ T‐cells, but not macrophages and CD8+ T‐cells compared to sham‐treated tumors. Notably, TZ tumors had a significantly lower density of neutrophils, CD4+ T‐cells, and CD8+ T‐cells, but not macrophages, when compared to RZ tumors.ConclusionsThe accelerated perinecrotic tumor outgrowth following RFA is associated with a reduced density of neutrophils and T‐cells compared to distant hepatic metastases. This may have implications for local tumor recurrence following RFA. J. Surg. Oncol. 2010; 101:551–556. © 2010 Wiley‐Liss, Inc.