bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological properties of an anti‐bacterial membrane for guided bone regeneration: an experimental study in rats
Authors: Zhang Jingchao , Xu Qian , Huang Cui , Mo Anchun , Li Jidong , Zuo Yi 
Published: January 2010

AbstractObjective: The biosafety and efficacy of silver–hydroxyapatite–titania/polyamide nanocomposite (nAg–HA–TiO2/PA) membrane as a guided bone regeneration (GBR) barrier were investigated based on a rat subcutaneous and critical‐size calvarial defect model.Material and methods: Thirty‐six Sprague–Dawley albino rats were divided into nAg–HA–TiO2/PA membrane test, expanded polytetrafluoroethylene (e‐PTFE) membrane control and blank control. Inflammatory response and bone regeneration in each group were evaluated using morphological, serological, radiographic and histological techniques at 1, 4 and 8 weeks, respectively, after implantation.Results: For subcutaneous implantation, slight degradation of nAg–HA–TiO2/PA membranes was observed by scanning electron microscope at 4 and 8 weeks. Histopathologic examination demonstrated a thinner layer of granulation tissue in the vicinity of nAg–HA–TiO2/PA membranes than that of e‐PTFE membranes. For cranial defect implantation, the serum alkaline phosphatase level was remarkably higher in nAg–HA–TiO2/PA group than that in e‐PTFE group. Radiographic and histomorphometric analysis showed a fully closed cranial defect for both nAg–HA–TiO2/PA and e‐PTFE groups at 8 weeks. No remarkable difference was found between the two groups regarding the integral optical density of neo‐bone at each time interval.Conclusion: nAg–HA–TiO2/PA membranes demonstrated better biocompatibility and similar osteoinductive activity compared with e‐PTFE membranes. nAg–HA–TiO2/PA composite membranes provided a good prospect for further research and development in anti‐bacterial GBR membrane. To cite this article:
Zhang JC, Xu Q, Huang C, Mo AC, Li JD, Zuo Y. Biological properties of an anti‐bacterial membrane for guided bone regeneration: an experimental study in rats. Clin. Oral Impl. Res. 21, 2010; 321–327. doi: 10.1111/j.1600‐0501.2009.01838.x
Early loading of implants with fixed dental prostheses in edentulous mandibles: 4.5‐year clinical results from a prospective study
Authors: Schwarz Stefanie , Gabbert Olaf , Hassel Alexander J. , Schmitter Marc , Séché Christiane , Rammelsberg Peter 
Published: January 2010

AbstractAim: The purpose of this study was to evaluate the survival and success of early‐loaded implants placed in the intraforaminal area of the edentulous mandible, and the survival of the implant‐supported fixed dental prostheses (FDP).Material and methods: Thirty‐seven patients (18.9% male, mean age 64.5 years) with edentulous lower jaws were treated with implant‐supported FDPs in the mandible. One hundred and eighty‐five screw‐type implants were placed in the intraforaminal area of the symphysis (five implants per patient). Immediately after implant placement, a framework was fabricated and the FDP was manufactured on the framework. Within 2 weeks, the implants were rigidly connected and loaded with the implant‐retained FDP.Results: During the 1–8‐year observation period (mean 4.5 years), a total of 32 implant‐retained complications occurred. Nineteen implants were lost in 10 patients, resulting in a cumulative survival of 89.7%. Nine implants in five patients did not osseointegrate. Although these implants were not removed, because stability within the connective tissue was acceptable and inflammation was absent, they were recorded as unsuccessful. Consequently, the cumulative success declined to 84.9%. Four implants in three patients had clinical signs of periimplantitis (2.2% of all implants). Denture‐related complications included one complete failure, when one FDP had to be removed after the last of five implants had been replaced. Furthermore, 10 fractures of the framework occurred in six patients, three FDPs had to be adapted or modified, and the facing of the FDP had to be repaired 16 times in 11 patients.Conclusion: Although one‐stage early‐loaded implants functioned well for most patients with edentulous mandibles, immediate loading is associated with a larger number of implant‐related complications than in other studies investigating delayed loading. Because of the substantial prosthetic complications and aftercare, this procedure cannot be generally recommended. To cite this article:
Schwarz S, Gabbert O, Hassel A J, Schmitter M, Séché C, Rammelsberg P. Early loading of implants with fixed dental prostheses in edentulous mandibles: 4.5‐year clinical results from a prospective study. Clin. Oral Impl. Res. 21, 2010; 284–289. doi: 10.1111/j.1600‐0501.2009.01843.x
Association between self‐efficacy and loss to follow‐up in long‐term periodontal treatment
Authors: Kakudate Naoki , Morita Manabu , Yamazaki Shin , Fukuhara Shunichi , Sugai Makoto , Nagayama Masato , Kawanami Masamitsu , Chiba Itsuo 
Published: January 2010

Kakudate N, Morita M, Yamazaki S, Fukuhara S, Sugai M, Nagayama M, Kawanami M, Chiba I. Association between self‐efficacy and loss to follow‐up in long‐term periodontal treatment. J Clin Periodontol 2010; 37: 276–282. doi: 10.1111/j.1600‐051X.2009.01529.xAbstractAim: To examine whether or not oral health care‐specific self‐efficacy can predict loss to follow‐up for long‐term periodontal treatment.Methods: Our prospective cohort study enrolled 144 patients with chronic periodontitis. Patient self‐efficacy was assessed on the initial visit using the self‐efficacy scale for self‐care (SESS). Participants were then followed for 30 months from the onset of periodontal treatment. The loss to follow‐up is the main outcome variable. Multiple logistic regression analysis was conducted to examine the association between self‐efficacy for self‐care and loss to follow‐up for periodontal treatment. Gender, age, number of teeth, probing depth, plaque control record (PCR) value, PCR improvement rate, general self‐efficacy scale score, and SESS score were used as the independent variables, and loss to follow‐up as a dependent variable.Results: A total of 67 patients were lost to follow‐up over the course of the study. Compared with the high‐scoring SESS group (60–75), the odds ratio of loss to follow‐up for the middle‐ (54–59) and low‐scoring groups (15–53) were 1.05 [95% confidence interval (CI): 0.36–3.07] and 4.56 (95% CI: 1.11–18.74), respectively.Conclusion: Assessment of oral health care‐specific self‐efficacy may be useful in predicting loss to follow‐up in long‐term periodontal treatment.
Child dental fear as measured with the Dental Subscale of the Children’s Fear Survey Schedule: the impact of referral status and type of informant (child versus parent)
Authors: Gustafsson Annika , Arnrup K. , Broberg A. G. , Bodin L. , Berggren U. 
Published: January 2010

Gustafsson A, Arnrup K, Broberg AG, Bodin L, Berggren U. Child dental fear as measured with the Dental Subscale of the Children’s Fear Survey Schedule: the impact of referral status and type of informant (child versus parent). Community Dent Oral Epidemiol 2010; 38: 256–266. © 2010 John Wiley & Sons A/SAbstract –  Objectives:  The first aim of this methodological study was to investigate the agreement between self‐ratings of Children’s Fear Survey Schedule (CFSS‐DS). The second aim was to explore using differentiated cut‐off scores, and to compare these cut‐off scores with those commonly used.Methods:  Three different data collections included study groups (n = 497) of children and adolescents who had been referred to specialized pediatric dentistry clinics, and reference groups (n = 499) of dental patients and children rating the analyses were limited to the Östergötland sample (n = 210 + 228). Patients and their accompanying parents (mainly mothers) were asked to fill in the CFSS‐DS independently. Cut‐off scores on the CFSS‐DS scale were determined using receiver‐operating characteristic analysis; patient–parent agreement was illustrated with Bland–Altman plots.Results:  The patient–parent agreement was modest, particularly among those who were referred because of dental behaviour management problems (DBMP). Cut‐off scores differentiated by age and gender, suggested by exploration according to two different methods, were with few exceptions clearly below the standard cut‐off score.Conlusion:  The validity of parental ratings of their children dental fear should be questioned, particularly in high‐fear populations. Self‐ratings should, as far as possible, be used to complement parental ratings. One consequence of using the standard cut‐off score is the risk of overlooking some patients needs for special attention. Further research is needed to establish and validate age‐and gender‐differentiated cut‐off scores on the CFSS‐DS.
Predictors of treatment outcomes after removal of amalgam fillings: associations between subjective symptoms, psychometric variables and mercury levels
Authors: Weidenhammer Wolfgang , Bornschein Susanne , Zilker Thomas , Eyer Florian , Melchart Dieter , Hausteiner Constanze 
Published: January 2010

Weidenhammer W, Bornschein S, Zilker T, Eyer F, Melchart D, Hausteiner C. Predictors of treatment outcomes after removal of amalgam fillings: associations between subjective symptoms, psychometric variables and mercury levels. Community Dent Oral Epidemiol 2010. © 2010 John Wiley & Sons A/SAbstract –  Objective:  This analysis aimed to study predictors of different treatment outcomes and associations between subjective symptoms, psychometric variables and mercury levels in patients who subjectively attributed their health problems to dental amalgam.Material and methods:  A secondary and retrospective analysis of data of a recently published randomized clinical trial was performed. Seventy‐eight patients [44% female, mean (SD) age 35 (6) years, randomly assigned to either amalgam removal or a health promotion program] were included into statistical analysis. Prior to intervention and 12 months later, questionnaires for assessing symptoms, psychological distress and health status were presented, and mercury levels in blood and urine were determined.Results:  The patients’ personality profile at study onset was characterized by slightly reduced extraversion and slightly elevated emotional instability. Overall, subjective symptoms decreased slightly and there were no statistically significant differences in the decrease of symptoms after intervention between both groups. Decrease of mercury levels after intervention was closely associated with removal of amalgam fillings (rmult = 0.64 in regression analysis). Statistically significant correlations could be found between mercury levels and subjective symptoms with respect to baseline (r = 0.29–0.39) and to changes after intervention (r = 0.24–0.42), but not for psychological distress (r = 0.05–0.25) and health related quality of life (r = −0.03–0.18). Prediction of symptom improvement after intervention was poor (rmult = 0.44).Conclusions:  Results contribute some new aspects to the inconsistent findings in the literature with respect to associations between symptoms and subtoxic mercury levels. More emphasis should be placed on exploring individual vulnerability for amalgam sensitivity.
Effective ex vivo expansion of hematopoietic stem cells using osteoblast‐differentiated mesenchymal stem cells is CXCL12 dependent
Authors: Mishima Seiji , Nagai Atsushi , Abdullah Sk , Matsuda Chikashi , Taketani Takeshi , Kumakura Shunichi , Shibata Hiroshi , Ishikura Hiroto , Kim Seung U. , Masuda Junichi 
Published: January 2010

AbstractEffective ex vivo expansion of hematopoietic stem cells (HSCs) is a prerequisite for HSC transplantation. Growth and maintenance of HSC is dependent on cytokine and niche factors. We investigated whether mesenchymal stem cells (MSCs) or osteogenic cytokine‐differentiated MSCs play a role in HSC expansion. We used the human HM3.B10 (B10) MSC cell line and the osteoblast‐differentiated B10 (Ost‐B10) as a feeder layer and examined ex vivo expansion of CD34+CD38− HSCs obtained from peripheral blood (PB) and cord blood (CB) with or without several growth cytokines. Both undifferentiated B10 and Ost‐B10 cells exhibited similar effects on total HSC expansion; however, Ost‐B10 demonstrated a higher potency in CD34+CD38− cell‐specific proliferation in the presence of cytokines compared to undifferentiated B10 HSCs. Colony‐forming cell assay and long‐term culture initiating cell assay revealed that Ost‐B10 displayed multipotent differentiation and enabled long‐term ex vivo culture of HSCs. We next examined the relationship between HSC expansion and the presence of various chemokines. CXCL4 and CXCL12 expression were increased in Ost‐B10 cells compared with the B10 cells. CD34+CD38− cells were significantly increased with CXCL12, but not CXCL4 treatment. siRNA inhibition of CXCL12 decreased CXCL12 secretion in both B10 and Ost‐B10, whereas expansion of CD34+CD38− cells was decreased in Ost‐B10 alone. These results demonstrated that ex vivo expansion of HSCs may be highly effective through osteoblast‐differentiated MSCs acting as a feeder layer, and likely operates through the CXCL12 chemokines signaling pathway.
Iteratio: calculating environmental indicator values for species and relevés
Authors: Holtland W. J. , Ter Braak C. J. F. , Schouten M. G. C. 
Published: January 2010

AbstractQuestion: Is it possible to translate vegetation maps into reliable thematic maps of site conditions?Method: This paper presents a new method, called Iteratio, by which a coherent spatial overview of specific environmental conditions can be obtained from a comprehensive vegetation survey of a specific area. Iteratio is a database application which calculates environmental indicator values for vegetation samples (relevés) on the basis of known indicator values of a limited number of plant species. The outcome is then linked to a digitalized vegetation map (map of plant communities) which results in a spatial overview of site conditions.Iteratio requires the indicator values of a minimum of 10–20% of the species occurring. The species are given a relative weight according to their amplitudes: species with a narrow range are weighted stronger, species with a broad range are weighted weaker.Conclusion: The method presented here enables a coherent assessment of site conditions on the basis of a vegetation survey and the indicator values of a limited number of plant species.
Strategic light manipulation as a restoration strategy to reduce alien grasses and encourage native regeneration in Hawaiian mesic forests
Authors: McDaniel S. , Ostertag R. 
Published: January 2010

AbstractQuestion: Is there a light level at which alien grass biomass is reduced while still supporting growth and survival of native woody species, allowing for native species regeneration in abandoned pastures?Location: Island of Hawaii, USA.Methods: In a two‐part study we examined the effect of light availability on common native woody and alien grass species found in secondary forests in Hawaii. A field survey was conducted to examine the relationship between light availability and canopy type (open pasture, planted canopy and secondary forest) on understory grass biomass and litter accumulation. We then experimentally manipulated light levels to determine the effect of light availability on growth and survival of six native woody species and three alien grasses. Low‐light (5%), medium‐light (10%) and high‐light (20‐30%) treatments were created using shade structures erected beneath the existing secondary koa canopy.Results: In the field survey, alien grass biomass was greatest under the open pasture and lowest in the secondary forest. There was a positive correlation between understory light availability and alien grass biomass. In the experimental study, large reductions in relative growth rates were documented for all of the grass species and four of the six woody species under the lowest light level. Although growth at 5% light is substantially reduced, survival is still high (84‐100%), indicating that these species may persist under closed canopy.Conclusion: Low‐light conditions result in the greatest reduction in alien grass biomass while creating an environment in which native woody species can grow and survive.
Original Research—Endocrinology: A Comparative Study of the Effects of Local Estrogen With or Without Local Testosterone on Vulvovaginal and Sexual Dysfunction in Postmenopausal Women
Authors: Raghunandan Chitra , Agrawal Swati , Dubey Priyanka , Choudhury Monisha , Jain Anju 
Published: January 2010

ABSTRACTIntroductionA significant number of postmenopausal women suffer from distressing problems because of urogenital atrophy secondary to the decline in circulating estrogen levels. Treatment with topical hormones may provide relief in such women when used judiciously.AimTo study the effects of local estrogen with or without local testosterone on urogenital and sexual health in postmenopausal women.MethodsSeventy-five postmenopausal women symptomatic for urogenital atrophy and sexual dysfunction were randomly divided into two study groups and one control group. The women in study group 1 received local estrogen cream; study group 2 received local estrogen and testosterone cream; the control group received nonhormonal lubricant KY gel for 12 weeks. The urogenital and sexuality score, along with the vaginal health index and the vaginal maturation index (VMI), was calculated at the beginning of therapy and 12 weeks later.Main Outcome MeasuresChanges in the urogenital and sexuality score along with vaginal health index and VMI.ResultsAfter 12 weeks of therapy, there was a significant improvement in all the four study parameters, which correlated well with the improvement in symptoms of urogenital atrophy and sexual dysfunction in both the study groups as compared with the control group. Improvement in sexuality score was greatest with combined estrogen–androgen therapy. There were no adverse effects and the therapies were well accepted without any compliance issue.ConclusionLocal estrogen either alone or with androgen is highly effective in relieving symptoms of urogenital atrophy and in improving sexual function in symptomatic postmenopausal women.
Strategically Managing Negotiation Linkage Dynamics
Authors: Crump Larry 
Published: January 2010

AbstractNegotiation linkage (the way in which one negotiation influences the process or outcome of another) presents challenges that are complex and real. Based on field research, this qualitative study examines four linked‐bilateral trade treaty negotiations conducted by Australia, Chile, the European Union, Singapore, and the United States to establish theoretical understanding about the strategic management of negotiation linkage dynamics. Several outcomes are achieved through case analysis. This study (a) introduces “degree of linkage dynamics” (robust, moderate, or modest) as a concept and concludes that it is determined by structural and contextual factors, (b) develops a framework of linked party action, (c) establishes guidance for managing opportunistic behavior in linked negotiations, (d) builds a six‐part typology of strategic techniques that can produce tangible gains in linked negotiations, and (e) examines research opportunities to further extend negotiation linkage theory. Research methodology developed in this study serves as a model for investigating negotiation linkage dynamics.
The Effects of Achievement Motivational Goals and of Debriefing on the Transfer of Skills in Integrative Negotiations
Authors: Bereby‐Meyer Yoella , Moran Simone , Sattler Liat 
Published: January 2010

AbstractIn this study, we examine the effects of achievement motivational goals (Learning vs. Performance) and of debriefing on the ability of trainees to acquire integrative (i.e., value‐creating) negotiation skills and to effectively transfer these skills across situations. Participants in four between‐subject conditions: 2 motivational goal conditions (learning/performance goals) × 2 debriefing conditions (with/without debriefing) first gained experience by engaging in repeated negotiations within an unchanging market simulation, and then proceeded to negotiate a more complex integrative negotiation task. A fifth control condition in which participants performed the second task only was also included. Experience accompanied by subsequent debriefing was found to be more effective for learning than experience without debriefing; it enhanced both integrative performance in the transfer task as well as conceptual understanding. Contrary to our expectations, participants who were assigned learning goals did not show an advantage over participants who were assigned performance goals.
Lower Predation Risk for Migratory Birds at High Latitudes
Authors: McKinnon L. , Smith P. A. , Nol E. , Martin J. L. , Doyle F. I. , Abraham K. F. , Gilchrist H. G. , Morrison R. I. G. , Bêty J. 
Published: January 2010

Predator Avoidance StrategySelective pressures influencing bird migration can include availability of food, pressure from parasites and pathogens, and predation risk. The importance of the last of these is revealed byMcKinnonet al.(p.326; see the Perspective byGilg and Yoccoz), who present an experimental analysis of the benefits of long-distance migration for reproduction in arctic-nesting birds. Measurements of a controlled effect of predation risk along a 3350-kilometer north-south gradient across arctic Canada provides evidence that the risk of nest predation decreases with latitude. Thus, birds migrating further north may acquire reproductive benefits in the form of reduced predation risk.
Use of Protein AG in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis ofToxoplasma gondiiInfection in Four Species of Animals
Authors: Zhang Donglin , Wang Zhengsong , Fang Rui , Nie Hao , Feng Huihui , Zhou Yanqin , Zhao Junlong 
Published: January 2010

ABSTRACTAn enzyme-linked immunosorbent assay (ELISA) employing protein AG (AG-ELISA) as a conjugate was developed to detect anti-Toxoplasma gondiiantibodies (Ab) in experimentally infected pigs and naturally infected pigs, goats, dogs, and cats. The results indicate that AG-ELISA can be a useful method for serological diagnosis ofT. gondiiinfection in these four species of animals.
Characterization of Host Responses against a Recombinant Fowlpox Virus-Vectored Vaccine Expressing the Hemagglutinin Antigen of an Avian Influenza Virus
Authors: Hghihghi Hamid R. , Read Leah R. , Mohammadi Hakimeh , Pei Yanlong , Ursprung Claudia , Nagy Éva , Behboudi Shahriar , Haeryfar S. M. Mansour , Sharif Shayan 
Published: January 2010

ABSTRACTThere currently are commercial fowlpox virus (FPV)-vectored vaccines for use in chickens, including TROVAC-AIV H5, which expresses the hemagglutinin (HA) antigen of an avian influenza virus and can confer immunity against avian influenza in chickens. Despite the use of recombinant FPV (rFPV) for vaccine delivery, very little is known about the immune responses generated by these viruses in chickens. The present study was designed to investigate host responses to rFPVin vivoandin vitro. In cultured cells infected with TROVAC-AIV H5, there was an early increase in the expression of type I interferons (IFN), Toll-like receptors 3 and 7 (TLR3 and TLR7, respectively), TRIF, and MyD88, which was followed by a decrease in the expression of these genes at later time points. There also was an increase in the expression of interleukin-1β (IL-1β), IL-8, and beta-defensin genes at early time points postinfection. In chickens immunized with TROVAC-AIV H5, there was higher expression of IFN-γ and IL-10 at day 5 postvaccination in spleen of vaccinated birds than in that of control birds. We further investigated the ability of the vaccine to induce immune responses against the HA antigen and discovered that there was a cell-mediated response elicited in vaccinated chickens against this antigen. The findings of this study demonstrate that FPV-vectored vaccines can elicit a repertoire of responses marked by the early expression of TLRs, type I interferons, and proinflammatory cytokines, as well as cytokines associated with adaptive immune responses. This study provides a platform for designing future generations of rFPV-vectored vaccines.