bluuebunny/crossref_metadata_2025_split huggingface dataset:
TPI ASM8 reduces eosinophil progenitors in sputum after allergen challenge
Authors: Imaoka H. , Campbell H. , Babirad I. , Watson R. M. , Mistry M. , Sehmi R. , Gauvreau G. M. 
Published: July 2011

Summary
Background TPI ASM8 contains two modified phosphorothioate antisense oligonucleotides (AON), one targeting the common beta chain (βc) of the IL‐3/IL‐5/GM‐CSF receptors and the other targeting the chemokine receptor CCR3. Inhalation of TPI ASM8 significantly improves lung function and sputum eosinophilia after allergen inhalation challenge in asthmatics.
Objective This study assessed whether TPI ASM8 reduces airway levels of haemopoietic progenitor cells.
Methods This open‐label study was conducted in 14 stable, allergic mild asthmatic subjects with early‐ and late‐phase allergen‐induced bronchoconstriction. Subjects underwent allergen challenges after 4‐day treatment with placebo, 4 mg b.i.d. and 8 mg o.d. of TPI ASM8. Sputum was induced before, 7 and 24 h after allergen challenges for progenitor measurements. Treatments were separated by 2–3 weeks.
Results TPI ASM8 reduced allergen‐induced sputum eosinophils, and the early and late asthmatic responses (P<0.05). TPI ASM8 also reduced the number of CD34+CCR3+ cells (P=0.004) and CD34+IL‐5Rα+ cells (P=0.016), and the proportion of CD34+ cells expressing IL‐5Rα (P=0.036).
Conclusions and Clinical Relevance TPI ASM8 was safe and well tolerated. The results of this study demonstrate blocking of CCR3 and βc expression by TPI ASM8 significantly inhibits the accumulation of eosinophils and eosinophil progenitors in the airways after allergen challenge. Inhibition of airway progenitor cell accumulation presents a novel therapeutic target.
Cite this as: H. Imaoka, H. Campbell, I. Babirad, R. M. Watson, M. Mistry, R. Sehmi and G. M. Gauvreau, Clinical & Experimental Allergy, 2011 (41) 1740–1746.
Aberrant plasmacytoid dendritic cell distribution and function in patients with Crohn's disease and ulcerative colitis
Authors: Baumgart D C , Metzke D , Guckelberger O , Pascher A , Grötzinger C , Przesdzing I , Dörffel Y , Schmitz J , Thomas S 
Published: July 2011

Summary
Dendritic cell (DC) function is believed to be of critical importance for the pathogenesis of inflammatory bowel disease (IBD). To date, most research in animal models and the few human data available is restricted to myeloid DC, while plasmacytoid DC (pDC) capable of controlling both innate and adaptive immune responses have not yet been investigated systematically in human Crohn's disease (CD) or ulcerative colitis (UC). CD11c−, CD303+/CD304+ and CD123+ pDC from peripheral blood (n = 90), mucosal tissue (n = 28) or mesenteric lymph nodes (n = 40) (MLNs) of patients with UC and CD or controls were purified and cultured. Thereafter, pDC were enumerated, phenotyped and cytokine secretion measured by flow cytometry (FACS), immunohistochemistry and/or cytometric bead array, respectively. Interferon (IFN)-α secretion following cytosine phosphatidyl guanine (CpG) A oligodeoxynucleotide (ODN) 2216 (5′-GGGGGACGATCGTCGGGGGG-3′) stimulation was assessed by enzyme-linked immunosorbent assay (ELISA). We found a significantly higher frequency of pDC in the inflamed colonic mucosa and MLN of IBD patients. Moreover, the fraction of CD40 and CD86 expressing cultured peripheral blood pDC was significantly higher in flaring UC and CD patients and their secretion of tumour necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 were increased significantly compared with controls. In contrast, the IFN-α secretion of peripheral blood pDC isolated from flaring IBD, particularly in UC patients, was reduced significantly compared with controls. Our data suggest an aberrant distribution and function of pDC in IBD, contrary to their generally implicated role as inducers of tolerance. We speculate that the impaired IFN-α secretion may relate to the hypothesized defect in innate immunity in IBD and could also impact upon the generation of regulatory T cells (Treg).
Biogeochemical properties of fine particulate organic matter as an indicator of local and catchment impacts on forested streams
Authors: Sakamaki Takashi , Richardson John S. 
Published: July 2011

Summary1. The magnitude and spatial scales of human effects on stream habitats need to be correctly measured to achieve sound ecosystem management. We investigated whether the biogeochemical properties of fine particulate organic matter (FPOM) might be indicative of reach‐scale vs. catchment‐scale effects on forested stream ecosystems.2. Along each of three forested streams, we established 4–5 sampling stations at 0·6–2·0‐km intervals, which represent a range of local riparian forest conditions (e.g. vegetation types and forestry practices). At each station, rock biofilm (considered as a representative of stream‐origin POM), FPOM in sediment (FPOMS) and suspended in water (FPOMW) and three species of benthic macroinvertebrates were collected during a summer low‐flow period.3. Measures of δ13C, C:N and chlorophyll a:C for FPOMW, FPOMS and biofilm were longitudinally heterogeneous, reflecting the reach‐scale, local environment. δ13C, C:N and chlorophyll a:C of FPOMS were significantly related to irradiance and streambed coarse particulate organic matter (CPOM) abundance, suggesting that the relative contribution of in‐stream primary production and CPOM breakdown are dominant controls of FPOM properties.4. In redundancy analysis, variations in FPOM properties were principally explained by local environmental factors correlated with stream size and/or longitudinal position of the sampling stations (irradiance, streambed CPOM, stream gradient, discharge, riparian vegetation). No significant effect of riparian forestry activities (logged, riparian reserve or no harvest in the past 75 years) was found on FPOM properties.5. Resource use by primary consumers was species dependent. δ13C of larval Lepidostoma roafi depended on the relative abundance of stream‐origin POM in pools of stored FPOM. Selective intake/assimilation of stream‐origin POM by this species was probably enhanced when stream‐origin POM was abundant. δ13C of larval Despaxia augusta or larval Paraleptophlebia spp. were not related to FPOMSδ13C, suggesting no linkage with FPOM composition.6. Synthesis and applications. The tight linkages between biogeochemical properties of FPOM, resource use by primary consumers, and reach‐scale environment indicate that local effects were greater than signals transmitted from upstream in this study. The use of in‐stream FPOM properties can help managers to measure reach‐scale effects of environmental changes on forested stream habitats.
ABO‐incompatible pediatric liver transplantation in very small recipients: Birmingham’s experience
Authors: Gelas Thomas , McKiernan Patrick J. , Kelly Deirdre A. , Mayer David A. , Mirza Darius F. , Sharif Khalid 
Published: July 2011

Gelas T, McKiernan PJ, Kelly DA, Mayer DA, Mirza DF, Sharif K. ABO‐incompatible pediatric liver transplantation in very small recipients: Birmingham’s experience. Pediatr Transplantation 2011: 15: 706–711. © 2011 John Wiley & Sons A/S.Abstract:  Liver transplantation (LT) for very small recipients is challenging but in experienced centres, good results can be achieved. Despite the risk of antibody‐mediated acute rejection, some studies have demonstrated the safety of ABO incompatible liver transplantation (ILT) in children and particularly in infants. The aim of our study was to describe the outcome of liver transplantation in infants <5 kg and the safety of using ILT in this group. All LT performed between 1991 and 2010 in children <5 kg were reviewed. Twenty‐nine patients were included, five of whom had an ILT. Acute liver failure was encountered in 20 cases. The recipient age and weight at transplantation were respectively 63 days (range: 14–268 days) and 4 kg (range: 2.4–5 kg). The graft‐to‐recipient ratio was 6.1% (range 2.3–9%). An aortic conduit and delayed abdominal closure were used respectively in 76% and 81% of the procedures. The ABO compatible liver transplantation (CLT) and ILT groups were similar regarding recipient’s demographics, graft types or technical transplantation data. The one‐ and five‐yr patient and graft survival were respectively 62%, 62% and 62%, 57.9% with a median follow‐up of 95 months. Vascular complications occurred in six cases (21.4%) and biliary complications were encountered in five patients (17%). Acute and chronic rejection developed respectively in 37% and 26% of the recipients. The five patients undergoing ILT are all alive without graft lost after a median follow‐up of 34 months (range 7–55 months). When compared with the CLT group, no significant differences were found regarding patient or graft survival, vascular or biliary complications and rejection rates. In our experience, ILT in small infants has short and long term outcomes comparable to ABO‐compatible grafts and excellent results can be achieved with a standard immunosuppressive protocol. To avoid mortality on the waiting list for neonatal recipients, ABO‐incompatible liver grafts can be used safely.
Favorable preliminary results using TLI/ATG‐based immunomodulatory conditioning for matched unrelated donor allogeneic hematopoietic stem cell transplantation in pediatric severe aplastic anemia
Authors: Pillai Asha , Hartford Christine , Wang Chong , Pei Deqing , Yang Jie , Srinivasan Ashok , Triplett Brandon , Dallas Mari , Leung Wing 
Published: July 2011

Pillai A, Hartford C, Wang C, Pei D, Yang J, Srinivasan A, Triplett B, Dallas M, Leung W. Favorable preliminary results using TLI/ATG‐based immunomodulatory conditioning for matched unrelated donor allogeneic hematopoietic stem cell transplantation in pediatric severe aplastic anemia. Pediatr Transplantation 2011: 15: 628–634. © 2011 John Wiley & Sons A/S.Abstract:  To assess whether a tolerance‐induction regimen could be applied for unrelated (MUD) HCT in SAA, we retrospectively reviewed our HCT experience using unmanipulated 10/10 HLA‐matched bone marrow grafts from MSD vs. MUD donors. Conditioning was CTX 200 mg/kg (CTX) + rabbit ATG 10 mg/kg (ATG) for MSD (n = 9) and TLI (800 cGy) + CTX/ATG for MUD HCT (n = 5). Immunoprophylaxis was CSA and short‐course MTX. Median patient age was 14.7 yr, median time to HCT 1.5 yr, and median follow‐up 3 yr. Outcome measures included EFS, time to engraftment, and cumulative incidence of GVHD (CIN of GVHD) for MSD and MUD cohorts. EFS and stable engraftment rate were 100%. CIN of acute GVHD was: MSD, Grade I–II: 1 (11%), Grade III–IV: 0%; MUD, Grade I–II: 1 (20%), Grade III–IV: 1 (20%). CIN of chronic GVHD was: MSD, limited: 1 (11%), extensive: 0%; MUD, limited: 0%, extensive: 0%. All immunosuppressive‐compliant patients successfully weaned immunosuppression. Although in limited patients, our results suggest that immunomodulatory TLI added to backbone CTX/ATG conditioning is a promising option for MUD HCT in SAA patients, which we will examine in a prospective clinical trial.
Total body irradiation and melphalan as a conditioning regimen for children with hematological malignancies undergoing transplantation with stem cells from HLA‐identical related donors
Authors: Watanabe Nobuhiro , Takahashi Yoshiyuki , Matsumoto Kimikazu , Horikoshi Yasuo , Hama Asahito , Muramatsu Hideki , Yoshida Nao , Yagasaki Hiroshi , Kudo Kazuko , Horibe Keizo , Kato Koji , Kojima Seiji 
Published: July 2011

Watanabe N, Takahashi Y, Matsumoto K, Horikoshi Y, Hama A, Muramatsu H, Yoshida N, Yagasaki H, Kudo K, Horibe K, Kato K, Kojima S. Total body irradiation and melphalan as a conditioning regimen for children with hematological malignancies undergoing transplantation with stem cells from HLA‐identical related donors. Pediatr Transplantation 2011: 15: 642–649. © 2011 John Wiley & Sons A/S.Abstract:  Although some studies have reported that TBI and MEL offer an effective conditioning regimen for autologous SCT in acute leukemia, little has been reported regarding outcomes of allogeneic SCT. We retrospectively evaluated outcomes for 50 pediatric patients who underwent allo‐SCT conditioned with intravenous MEL (180–210 mg/m2) and fractionated TBI (12–13.2 Gy) from HLA‐identical related donors. Nineteen patients were in CR1, 18 were in CR2, and 13 showed advanced‐stage disease (≥CR3). Patients had received allo‐SCT from HLA‐identical siblings (n = 45) or phenotypically HLA‐identical family donors (n = 5). Median duration of follow‐up for all disease‐free patients was 61 months (range, 8.8–177 months). At the time of analysis, 12 patients had died. Eleven of those died of relapse, and one died of TRM. DFS rates for all patients, patients with AML (n = 12), and patients with lymphoid malignancy (n = 38) were 61.4% and 82.1%, respectively. DFS rates for CR1, CR2, and ≥CR3 cases were 89.2%, 88.1%, and 23.1%, respectively (p < 0.05). MEL/TBI for pediatric patients with hematological malignancies was associated with lower relapse rates and no increase in toxicity, resulting in better survival.
Finding middle ground: negotiating university and tribal community interests in community‐based participatory research
Authors: Mohammed Selina A. , Walters Karina L. , LaMarr June , Evans‐Campbell Teresa , Fryberg Sheryl 
Published: July 2011

MOHAMMED SA, WALTERS KL, LAMARR J, EVANS‐CAMPBELL T and FRYBERG S. Nursing Inquiry 2012; 19: 116–127 [Epub ahead of print]Finding middle ground: negotiating university and tribal community interests in community‐based participatory researchCommunity‐based participatory research (CBPR) has been hailed as an alternative approach to one‐sided research endeavors that have traditionally been conducted on communities as opposed to with them. Although CBPR engenders numerous relationship strengths, through its emphasis on co‐sharing, mutual benefit, and community capacity building, it is often challenging as well. In this article, we describe some of the challenges of implementing CBPR in a research project designed to prevent cardiovascular disease among an indigenous community in the Pacific Northwest of the United States and how we addressed them. Specifically, we highlight the process of collaboratively constructing a Research Protocol/Data Sharing Agreement and qualitative interview guide that addressed the concerns of both university and tribal community constituents. Establishing these two items was a process of negotiation that required: (i) balancing of individual, occupational, research, and community interests; (ii) definition of terminology (e.g., ownership of data); and (iii) extensive consideration of how to best protect research participants. Finding middle ground in CBPR requires research partners to examine and articulate their own assumptions and expectations, and nurture a relationship based on compromise to effectively meet the needs of each group.
‘In a way, you have to pull the patient out of that state …’: the competency of ventilator weaning
Authors: Haugdahl Hege S , Storli Sissel L 
Published: July 2011

HAUGDAHL HS and STORLI SL. Nursing Inquiry 2012; 19: 238–246  ‘In a way, you have to pull the patient out of that state …’: the competency of ventilator weaningThe introduction of the weaning protocol has reduced weaning time and improved results in patients. However, the evidence is inconsistent. This may reflect that the use of a protocol should not exclude individual considerations and clinical judgement. However, the significant aspects of the context and the competency important in the nurse–patient relationship in weaning have not yet been sufficiently described. This study aimed at exploring these aspects of weaning. Qualitative data from six in‐depth interviews and field observations of three experienced intensive care nurses in weaning situations were analysed through systematic text condensation within a hermeneutic‐phenomenological approach. Competency appeared to be based on thorough knowledge of physiology and ventilator skills, but also on knowing the patient, helping the nurse connect the meaningless to the meaningful for the patient. Behaving competently involves a continuous dialogue with the situation, observation of the patient’s body language and symptoms over a period of time and the ability to see the interrelationships of all these elements. Competency in ventilator weaning may thus be linked to personal qualifications, while it is simultaneously dependent on a professional community that both confirms and acknowledges this competency.
A methylation assay for the detection of non‐muscle‐invasive bladder cancer (NMIBC) recurrences in voided urine
Authors: Zuiverloon Tahlita C.M. , Beukers Willemien , van der Keur Kirstin A. , Munoz Jesrael R. , Bangma Chris H. , Lingsma Hester F. , Eijkemans Marinus J.C. , Schouten Jan P. , Zwarthoff Ellen C. 
Published: July 2011

What's known on the subject? and What does the study add?Multiple studies report on the detection of methylation in voided urine samples as a possible approach for the follow‐up of non‐muscle invasive bladder cancer patients. Previous studies analyze methylation gene panels in a mixture of primary and recurrent tumours. As primary tumours are larger than recurrent tumours and thus easier to detect in urine, validation of methylation markers in urine samples from patients with primary tumours will result in a test sensitivity that does not reflect the true sensitivity of the assay.This study is the first to select a subset of genes specifically methylated in non‐muscle invasive bladder cancer recurrences and validates the gene panel in two independent sets of urine samples from recurrent patients, thus simulating the disease course according to the clinical presentation.OBJECTIVE
To develop a methylation‐specific multiplex ligation‐dependent probe amplification (MS‐MLPA) assay for the detection of non‐muscle invasive bladder cancer (NMIBC) recurrences in voided urine.
PATIENTS AND METHODS
Genes frequently methylated in NMIBC tumours (n= 37) were selected to develop a BC‐specific MS‐MLPA assay.
Genes methylated in blood from patientswith BC (n= 29) and genes methylated in urine from patients with no history of BC (n= 46) were excluded.
A four‐gene panel with the highest predictive value was selected from the initial assay. This four‐gene panel was tested and validated on urine from patients with a histologically confirmed recurrence (n= 68 test set; n= 49 validation set) and urine samples from patients without BC (n= 91, test set) and urine from recurrence‐free BC (rec‐free BC) patients (n= 60, validation set).
A model was developed to predict the probability of having a recurrence based on methylation of the four‐gene panel and a threshold probability with the highest sensitivity and specificity was determined.
The outcome of the model was validated on BC urine samples (n= 65) and on urine samples from rec‐free BC patients (n= 29).
RESULTS
The BC MS‐MLPA assay consisted of 23 methylation probes. The selected four‐gene panel included: APC_a, TERT_a, TERT_b, and EDNRB. This panel reached an area under the receiver operating characteristic curve (AUC) of 0.82 (test set) and AUC 0.69 (validation set). Sensitivity and specificity for the detection of a concomitant tumour were 63.3% and 58.3% respectively (test set) and 72.3% and 55.2%, respectively (validation set).
CONCLUSIONS
We have developed a methylation detection assay specifically for the detection of recurrences in patients with NMIBC in voided urine.
The findings are promising and improvement of this test could eventually contribute to a more individualized patient friendly surveillance.
Different levels of psychotherapeutic competence
Authors: Fruggeri Laura 
Published: July 2011

In this article, I argue for a both – and position in regard to manuals. I suggest that manuals are appropriate when describing therapeutic strategizing and technical competence. However, I also argue that they are an inappropriate tool for understanding the contribution of the client's family in family therapy. This argument fuels a further view that manuals cannot capture the dance that occurs between therapist and family. For this type of knowledge, a relational type of competence needs to be developed.We encourage you to listen to the JFT Editor, Mark Rivett, as he interviews the author on Manuals in the Practice and Research of Family Therapy. Available at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-6427/homepage/jft_podcast_series.htm.
Progressive Expectation‐Maximization for Hierarchical Volumetric Photon Mapping
Authors: Jakob Wenzel , Regg Christian , Jarosz Wojciech 
Published: July 2011

AbstractState‐of‐the‐art density estimation methods for rendering participating media rely on a dense photon representation of the radiance distribution within a scene. A critical bottleneck of such kernel‐based approaches is the excessive number of photons that are required in practice to resolve fine illumination details, while controlling the amount of noise. In this paper, we propose a parametric density estimation technique that represents radiance using a hierarchical Gaussian mixture. We efficiently obtain the coefficients of this mixture using a progressive and accelerated form of the Expectation‐Maximization algorithm. After this step, we are able to create noise‐free renderings of high‐frequency illumination using only a few thousand Gaussian terms, where millions of photons are traditionally required. Temporal coherence is trivially supported within this framework, and the compact footprint is also useful in the context of real‐time visualization. We demonstrate a hierarchical ray tracing‐based implementation, as well as a fast splatting approach that can interactively render animated volume caustics.
Regional cortical thickness and cognitive functions in non‐demented Parkinson’s disease patients: a pilot study
Authors: Pellicano C. , Assogna F. , Piras F. , Caltagirone C. , Pontieri F. E. , Spalletta G. 
Published: July 2011

Background and purpose:  The pathology of neuropsychological deficits in Parkinson’s disease (PD) is incompletely defined.Methods:  We investigated cortical thickness and neuropsychological performances in non‐demented patients with PD and healthy controls.Results:  Patients showed significant cortical thinning in right middle temporal and left fusiform cortices. Verbal memory performance was related with left fusiform thinning.Conclusions:  Cognitive and cortical changes in non‐demented patients with PD are detectable and clearly related.