bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Intramuscular Testosterone Undecanoate on Body Composition and Bone Mineral Density in Female-to-Male Transsexuals
Authors: Mueller Andreas , Haeberle Lothar , Zollver Hendryk , Claassen Tomma , Kronawitter Desiree , Oppelt Patricia G. , Cupisti Susanne , Beckmann Matthias W. , Dittrich Ralf 
Published: June 2010

ABSTRACT

Introduction
The most common treatment regimen in female-to-male transsexuals is administration of short-acting testosterone esters intramuscularly every 2 weeks.

Aim
The aim of this study was to evaluate the effect of long-acting intramuscular testosterone undecanoate on body composition and bone mineral density during cross-sex hormone therapy in female-to-male transsexuals.

Methods
Forty-five female-to-male transsexuals (FtMs) were treated with injections of testosterone undecanoate 1,000 mg intramuscularly every 12 weeks over 24 months.

Main Outcome Measures
Body composition, bone mineral density, hormone parameters, and lipids were compared after 12 months and after 24 months with baseline values. Sonographic findings in the ovaries and endometrium, clinical and adverse effects during the study period were recorded.

Results
There was a significant increase in lean mass in the FtMs during the study period in comparison with baseline values, whereas no change in BMI, fat mass, and bone mineral density was observed. There was a significant decline in gonadotropins, estradiol, dehydroepiandrosterone sulphate, sex hormone-binding globulin, and high-density lipoprotein, while testosterone and triglyceride levels increased significantly after 12 and 24 months. Ovaries remained unchanged and no noticeable endometrial pathology was observed. No mortality or morbidity was observed during the study period. We observed a cessation of menstrual bleeding, an increase in clitoral growth, libido, body and beard hair growth, deepened voices and decline in breast size. There was a significant increase in hemoglobin, hematocrit, glutamic-pyruvic transaminase, gamma-glutamyl transferase, and an increase in systolic blood pressure during the study period.

Conclusions
There was an increase in lean mass during the study period in FtMs treated with testosterone undecanoate. Transsexual patients should be monitored for adverse effects on lipid profiles, blood pressure, and erythrocytosis during intramuscular testosterone undecanoate therapy.
Greater Tactile Sensitivity and Less Use of Immature Psychological Defense Mechanisms Predict Women’s Penile-Vaginal Intercourse Orgasm
Authors: Brody Stuart , Houde Stephanie , Hess Ursula 
Published: June 2010

ABSTRACTIntroductionPrevious research has suggested that diminished tactile sensitivity might be associated with reduced sexual activity and function. Research has also demonstrated significant physiological and psychological differences between sexual behaviors, including immature psychological defense mechanisms (associated with various psychopathologies) impairing specifically women’s orgasm from penile-vaginal intercourse (PVI).AimsTo examine the extent to which orgasm triggered by PVI (distinguished from other sexual activities) is associated with both greater tactile sensitivity and lesser use of immature psychological defenses.MethodsSeventy French-Canadian female university students (aged 18–30) had their finger sensitivity measured with von Frey type microfilaments, completed the Defense Style Questionnaire and a short form of the Marlowe-Crowne social desirability scale, and provided details of the 1 month (and ever) frequencies of engaging in, and having an orgasm from, PVI, masturbation, anal intercourse, partner masturbation, and cunnilingus.Main Outcome MeasuresLogistic and linear regression prediction of orgasm triggered by PVI from tactile sensitivity, age, social desirability responding, and immature psychological defenses.ResultsHaving a PVI orgasm in the past month was associated with greater tactile sensitivity (odds ratio=4.0 for each filament point) and less use of immature defense mechanisms (odds ratio=5.1 for each scale point). Lifetime PVI orgasm was associated only with less use of immature defense mechanisms (and lower social desirability responding score). Orgasms triggered by other activities were not associated with either tactile sensitivity or immature defense mechanisms. Tactile sensitivity was also associated with greater past month PVI frequency (inclusion of PVI frequency in a logistic regression model displaced tactile sensitivity), and lesser use of immature defenses was associated with greater past month PVI and PVI orgasm frequencies.ConclusionsBoth diminished physical sensitivity and the presence of specific psychological impairments might decrease the likelihood of women’s orgasm from specifically PVI, but not other sexual activities.
Intracavernous Delivery of Synthetic Angiopoietin-1 Protein as a Novel Therapeutic Strategy for Erectile Dysfunction in the Type II Diabetic db/db Mouse
Authors: Jin Hai-Rong , Kim Woo Jean , Song Jae Sook , Piao Shuguang , Tumurbaatar Munkhbayar , Shin Sun Hwa , Choi Min Ji , Tuvshintur Buyankhuu , Song Kang-Moon , Kwon Mi-Hye , Yin Guo Nan , Koh Gou Young , Ryu Ji-Kan , Suh Jun-Kyu 
Published: June 2010

ABSTRACT

Introduction
Patients with erectile dysfunction (ED) associated with type II diabetes often have impaired endothelial function and tend to respond poorly to oral phosphodiesterase type 5 inhibitors. Therefore, neovascularization is a promising strategy for curing diabetic ED.

Aim
To determine the effectiveness of a soluble, stable, and potent angiopoietin-1 (Ang1) variant, cartilage oligomeric matrix protein (COMP)-Ang1, in promoting cavernous angiogenesis and erectile function in a mouse model of type II diabetic ED.

Methods
Sixteen-week-old male db/db mice (in which obesity and type II diabetes are caused by a mutation in the leptin receptor) and control C57BL/6J mice were used and divided into four groups (N=14 per group): age-matched controls; db/db mice receiving two successive intracavernous injections of phosphate-buffered saline (PBS) (days −3 and 0; 20 µL); db/db mice receiving a single intracavernous injection of COMP-Ang1 protein (day 0; 5.8 µg/20 µL); and db/db mice receiving two successive intracavernous injections of COMP-Ang1 protein (days −3 and 0; 5.8 µg/20 µL).

Main Outcome Measures
Two weeks later, erectile function was measured by electrical stimulation of the cavernous nerve. The penis was then harvested and stained with antibodies to platelet/endothelial cell adhesion molecule-1 (PECAM-1) (endothelial cell marker), phosphohistone H3 (PH3, a nuclear protein indicative of cell proliferation), phospho-endothelial nitric oxide synthase (eNOS), and eNOS. Penis specimens from a separate group of animals were used for cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) quantification.

Results
Local delivery of COMP-Ang1 protein significantly increased eNOS phosphorylation and cGMP and cAMP expression compared with that in the group treated with PBS. Repeated intracavernous injections of COMP-Ang1 protein completely restored erectile function and cavernous endothelial content through enhanced cavernous neoangiogenesis as evaluated by PECAM-1 and PH3 immunohistochemistry and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay, whereas a single injection of COMP-Ang1 protein elicited partial improvement.

Conclusion
Cavernous neovascularization using recombinant Ang1 protein is a novel therapeutic strategy for the treatment of ED resulting from type II diabetes. Jin H-R, Kim WJ, Song JS, Piao S, Tumurbaatar M, Shin SH, Choi MJ, Tuvshintur B, Song K-M, Kwon M-H, Yin GN, Koh GY, Ryu J-K, and Suh J-K. Intracavernous delivery of synthetic angiopoietin-1 protein as a novel therapeutic strategy for erectile dysfunction in the type II diabetic db/db mouse.
SURVIVAL OF SALMONELLA TYPHIMURIUM DURING THE RIPENING OF HERBY CHEESE (OTLU PEYNIR)
Authors: ALEMDAR SÜLEYMAN , AĞAOĞLU SEMA 
Published: June 2010

ABSTRACT This study was conducted to determine the survival of Salmonella Typhimurium during the ripening period of herby cheese made traditionally from raw milk. For this purpose, the cheese milk was inoculated with S. Typhimurium at the level of 103 and 105 colony‐forming units/mL, and then both manufactured herby cheeses were divided into two groups equally. The herby cheeses were stored by using two different methods for ripening, either embedding into the soil or putting into brine, and analyzed on the 1st, 7th, 15th, 30th, 60th and 90th days of ripening period. At the end of the storage period, S.Typhimurium could not be detected in embedded herby cheese at both levels of the inoculation; whereas it was found that the number of the bacterium was just decreased to 2.60 and 3.15 log MPN/g in brined herby cheese for each inoculation levels, respectively. The results indicated that S. Typhimurium could survive at least 60 days in embedded herby cheese and till the last days of the ripening in brined herby cheese. This point should be taken into account for the potential risk to public health.PRACTICAL APPLICATIONSMilk and other dairy products may be contaminated with some pathogenic bacteria such as Salmonella spp. Salmonella Typhimurium is an emerging food pathogen in cheeses when it contaminates the milk. In this study, the behavior of S. Typhimurium was investigated during ripening of herby cheeses. Herby cheese made traditionally from raw milk were stored either embedding into the soil or putting into brine. As a result, it was found that embedded cheeses were safer than brined cheeses with respect to the S. Typhimurium contamination at the end of the ripening period.
The bridge: Providing nursing care for survivors of sexual violence
Authors: Ross Ratchneewan , Draucker Claire Burke , Martsolf Donna , Adamle Kathleen , Chiang‐Hanisko Lenny , Lewandowski Wendy 
Published: June 2010

Abstract Purpose: To describe the experiences of nurses who provide care for survivors of sexual violence (SV) on a number of different types of hospital units. Data sources: Semi‐structured interviews of 23 unit managers and 12 advanced practice nurses from four tertiary care hospitals were audiotaped and transcribed. Participants were asked to describe how they screen for SV and how they provide care for survivors. A team approach was used to conduct a thematic analysis of the data. Conclusions: The bridge metaphor was chosen as the central theme of the data because it captures three key aspects of the participants’ experiences: an initial sense of disconnection from patients who are survivors of SV (being separated on opposite shores), the need to proceed cautiously (tentatively stepping onto the bridge), and the experience of forming a meaningful connection that serves as the context of care (meeting on the bridge and crossing it together). Implications for practice: This research suggests that nurses provide quality care for survivors of SV by intuitively responding to survivors’ cues of distress, cautiously broaching the topic of violence, and finding ways to ease the healthcare encounter.
Relationships between aggression, depression, and alcohol, tobacco: Implications for healthcare providers in student health
Authors: Roberts Susan J. , Glod Carol A. , Kim Reo , Hounchell Julie 
Published: June 2010

Abstract Purpose: To determine the prevalence of aggression, depression, and at‐risk health behaviors in a random sample of undergraduate college students and to explore the relationship between these variables. Data sources: The study survey was sent to 2500 undergraduate students; 428 participated, responding to items from the National College Health Risk Behavior Survey about alcohol, drug and tobacco, violence and aggression, the Beck Depression Inventory II, and items adapted from the Overt Aggression Scale. Conclusions: Almost one third of the sample reported cigarette smoking, 22% moderate depression, 81% drink alcohol, with 58% drinking more than five drinks at least once in the last month. Reports of verbal and physical aggression were also common. Moderate depression was related to cigarette smoking, physical, and verbal aggression, but not to heavy alcohol use. An understanding of these relationships can be utilized to screen and intervene with students at risk. Implications for practice: The results call for increased screening and treatment of depression in college students, and suggest that students with aggressive behaviors are at the highest risk for depression, and should be a group to receive specific attention for screening.
Pre‐ and posttest evaluation of a breast cancer risk assessment program for nurse practitioners
Authors: Edwards Quannetta T. , Seibert Diane 
Published: June 2010

Abstract Purpose: Numerous studies have shown that healthcare providers, including nurse practitioners (NPs) fail to provide breast cancer risk assessment (BrCRA) in primary care settings. A potential barrier to the use of BrCRA is insufficient knowledge or training of risk assessment. The purpose of this study was to analyze the outcome of a BrCRA program developed to enhance NPs' knowledge of risk assessment and use of empiric risk assessment models. Data sources: Thirty‐five NPs participated in a before‐after (pretest–posttest design) study evaluating the effectiveness of a BrCRA education program conducted at a national NP conference. Demographics, pre/post knowledge, and course satisfaction measures were all examined as a part of this pilot study. Conclusion: Continuing education through the implementation of a BrCRA program significantly increased NPs knowledge in assessing breast cancer risk and the use of empiric risk assessment models. Implications for practice: Many healthcare providers, including NPs, are inadequately prepared to assess a woman's risk for breast cancer. Understanding breast cancer risk assessment is essential if NPs are to provide appropriate counseling, management, and referral strategies needed to reduce a woman's risk for developing the disease. Continuing education provides one means to enhance NP's knowledge of BrCRA.
Making the diagnosis: Idiopathic rapid eye movement sleep behavior disorder
Authors: Taub Leslie‐Faith M. 
Published: June 2010

Abstract Purpose: To present a clinical case of idiopathic rapid eye movement sleep behavior disorder (RBD), differential diagnoses, selected treatments, and the pathology involved. Data sources: An Ovid data base search (covering 635 medical, neurologic, and psychiatric journals) was conducted using the search term RBD and limited to the years 2005–2009. This search strategy was used to locate clinical, research, and review articles providing the state of the science about RBD. A hand search was also conducted for seminal research papers as well as recent publications within the specialty of sleep disorders. Conclusions: RBD is a parasomnia with symptoms common to other disorders making it important to identify unique symptoms and diagnostic testing that helps differentiates these diseases. RBD can put the patient and the bed partner at risk for injury. Its prevalence is estimated to be 0.5% in older adults. Implications for practice: Nurse practitioners are primary care providers for older adults who may have complaints about behaviors associated with RBD. Knowledge of this disease process as well as its relationship with four other neurodegenerative diseases may provide an opportunity for early diagnosis and treatment of RBD and surveillance for and early diagnosis of the other neurodegenerative diseases in these patients.
Evaluating pressure ulcer care by home healthcare nurses
Authors: Yamamoto‐Mitani Noriko , Katakura Naoko , Fujita Junko , Shinohara Yuko , Sonoda Yoshimi , Hayashi Kunihiko 
Published: June 2010

yamamoto‐mitani n., katakura n., fujita j., shinohara y., sonoda y. & hayashi k. (2011) Evaluating pressure ulcer care by home healthcare nurses. International Journal of Older People Nursing6, 201–215doi: 10.1111/j.1748‐3743.2010.00228.xAim.  To field test an objective evaluation tool consisting of process‐oriented quality indicators for pressure ulcer care, using nursing charts of homecare nurses. These quality indicators were developed by the authors.Background.  Most Japanese homecare nursing agencies are small and to use much of their human and economic resources to improve the care quality is not realistic. A simple and effective system for quality assurance/improvement needs to be considered.Design.  Descriptive study design, using the chart reviews of 34 homecare nursing clients from two homecare nursing agencies.Methods.  Nursing charts were evaluated using our quality indicators for pressure ulcer care, and whether the charts pass or not in terms of the practices described in the quality indicator was rated. The pass rates by chart and nurses’ self‐evaluation were compared, and pass rates by charts were examined.Results.  The evaluation by chart review generally matched with self‐evaluations. The pass rates by charts were higher for indicators related to wound treatment than those related to preventive care.Conclusions and implications for practice.  Home healthcare nurses could give more attention to pressure ulcer prevention. Regular self‐checks of quality indicators may remind the nurses of the importance of prevention.
Insights gained through Marte Meo counselling: experiences of nurses in dementia specific care units
Authors: Einang Alnes Rigmor , Kirkevold Marit , Skovdahl Kirsti 
Published: June 2010

Alnes R.E., Kirkevold M. & Skovdahl K. (2010) Insights gained through Marte Meo counselling: experiences of nurses in dementia specific care units. International Journal of Older People Nursing6, 123–132 doi: 10.1111/j.1748‐3743.2010.00229.xAim.  This study sought to uncover what nurses perceived to have learned, during their participation in video supported counselling, based on Marte Meo principles, in four dementia specific care units.Methods.  This was a descriptive qualitative study. Data were collected through 12 individual and four focus group interviews. In addition, supplementary data from two video recordings and one written log were included. Findings emerged through content analysis and re‐examination of the text based on the initial analysis.Results.  The nurses experienced that they acquired new knowledge about the residents through Marte Meo Counselling (MMC), resulting in improved capability to interpret the residents` expressions, and increased awareness of the residents’ competence. New knowledge about themselves as nurses also emerged; they recognised how their actions entailed consequences for the interaction, in turn making them conscious of the usefulness of taking time, pacing their interactions, maintaining eye contact and describing the situation in words when the interaction took place. This appeared to increase the resident’s perception of being able to cope.Conclusions.  This study indicates that MMC helped the nurses to gain knowledge about how to improve interactions with residents suffering from dementia. Further research is warranted into the effectiveness of MMC.
The phage‐driven microbial loop in petroleum bioremediation
Authors: Rosenberg Eugene , Bittan‐Banin Gili , Sharon Gil , Shon Avital , Hershko Galit , Levy Itzik , Ron Eliora Z. 
Published: June 2010

SummaryDuring the drilling process and transport of crude oil, water mixes with the petroleum. At oil terminals, the water settles to the bottom of storage tanks. This drainage water is contaminated with emulsified oil and water‐soluble hydrocarbons and must be treated before it can be released into the environment. In this study, we tested the efficiency of a continuous flow, two‐stage bioreactor for treating drainage water from an Israeli oil terminal. The bioreactor removed all of the ammonia, 93% of the sulfide and converted 90% of the total organic carbon (TOC) into carbon dioxide. SYBR Gold staining indicated that reactor 1 contained 1.7 × 108 bacteria and 3.7 × 108 phages per millilitre, and reactor 2 contained 1.3 × 108 bacteria and 1.7 × 109 phages per millilitre. The unexpectedly high mineralization of TOC and high concentration of phage in reactor 2 support the concept of a phage‐driven microbial loop in the bioremediation of the drainage water. In general, application of this concept in bioremediation of contaminated water has the potential to increase the efficiency of processes.
Studies with bioengineered Nisin peptides highlight the broad‐spectrum potency of Nisin V
Authors: Field Des , Quigley Lisa , O'Connor Paula M. , Rea Mary C. , Daly Karen , Cotter Paul D. , Hill Colin , Ross R. Paul 
Published: June 2010

SummaryNisin A is the most thoroughly investigated member of the lantibiotic family of antimicrobial peptides. In addition to a long history of safe use as a food antimicrobial, its activity against multi‐drug resistant pathogens has resulted in a renewed interest in applying nisin as a chemotherapeutic to treat bacterial infections. The wealth of Nisin‐related information that has been generated has also led to the development of the biotechnological capacity to engineer novel Nisin variants with a view to improving the function and physicochemical properties of this already potent peptide. However, the identification of bioengineered Nisin derivatives with enhanced antimicrobial activity against Gram‐positive targets is a recent event. In this study, we created stable producers of the most promising derivatives of Nisin A generated to date [M21V (hereafter Nisin V) and K22T (hereafter Nisin T)] and assessed their potency against a range of drug‐resistant clinical, veterinary and food pathogens. Nisin T exhibited increased activity against all veterinary isolates, including streptococci and staphylococci, and against a number of multi‐drug resistant clinical isolates including MRSA, but not vancomycin‐resistant enterococci. In contrast, Nisin V displayed increased potency against all targets tested including hVISA strains and the hyper‐virulent Clostridium difficile ribotype 027 and against important food pathogens such as Listeria monocytogenes and Bacillus cereus. Significantly, this enhanced activity was validated in a model food system against L. monocytogenes. We conclude that Nisin V possesses significant potential as a novel preservative or chemotherapeutic compound.
Evaluation of a Fiber Reinforced Drillable Bone Cement for Screw Augmentation in a Sheep Model—Mechanical Testing
Authors: Ahern Benjamin J. , Harten Robert D. , Gruskin Elliott A. , Schaer Thomas P. 
Published: June 2010

AbstractWe evaluated the mechanical properties of a novel fiber reinforced calcium phosphate at time zero and after 12 weeks in vivo using a sheep long bone osteotomy model. Time zero data were obtained and compared by pullout testing of 4.5 mm bone screws from bone proper and overdrilled defects of 4.5 and 8 mm diameter. Defects were augmented with: polymethylmethacrylate (PMMA), calcium phosphate, and fiber reinforced calcium phosphate using cadaveric sheep tibiae. Twelve‐week data were obtained from explanted tibiae of sheep that underwent unilateral tibial osteotomy surgery repaired with a locking compression plate. The most distal hole was overdrilled to 4.5 or 8 mm diameter, filled with fiber reinforced cement, drilled, tapped and a 4.5 mm screw was placed. Screw holding strength at t= 0 was significantly higher for reinforced when compared to nonreinforced cement, but not different from bone or PMMA in 4.5 mm defects. There was no difference in pullout strength for the 8 mm defect data. After 12 weeks fiber reinforced pullout strength increased by 45% and 8.9% for 4.5 and 8 mm defects, respectively, when compared to t= 0 testing. Fiber reinforced calcium phosphate bone cement can be drilled and tapped to support orthopedic hardware for trauma applications. Clin Trans Sci 2010; Volume 3: 112–115