bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Bonding in Al5Co2: The Electron Localizability–Electron Density Approach
Authors: Ormeci Alim , Grin Yuri 
Published: December 2011

AbstractAl5Co2 is an intermetallic compound which can be considered as an approximant of the quasicrystalline decagonal phases. Chemical bonding in Al5Co2 is investigated by using the electron localizability–electron density approach. The analysis reveals that two atomic clusters, one three dimensional, the other planar, can be identified on the basis of two‐centre bonds that are formed around the shortest Al–Co distances. These clusters and one of the Al types interact through multi‐centre bonds, indicating that both covalent and metallic interactions coexist in Al5Co2.
IMPACTS OF DIRECT SEEDING INTO MULCH ON THE YIELD, WATER USE EFFICIENCY AND NITROGEN DYNAMICS OF CORN, SORGHUM AND DURUM WHEAT
Authors: Khaledian M. R. , Mailhol J. C. , Ruelle P. , Mubarak I. 
Published: December 2011

ABSTRACTDirect seeding into mulch (DSM) is a cropping system that is increasingly used in the world. The major concern among producers is the possible yield penalties associated with DSM compared to conventional tillage (CT). The aim of our study was to assess the effects of DSM on the yields of corn, sorghum and durum wheat, as well as water use efficiency (WUE) and nitrogen dynamics. An experimental study was carried out at the Lavalette experimental station from 2001 to 2007. Corn, sorghum and durum wheat were sown in two tillage treatments (CT and DSM). Yield and some yield components, nitrogen dynamics and WUE were determined. The results showed that DSM could maintain the crop production of corn and sorghum, however, for durum wheat, CT performed better. DSM improved WUE and irrigation water use efficiency (IWUE) of corn and sorghum and decreased crop evapotranspiration from 5 to 27%. These results suggest that DSM is a relevant alternative to the CT system for corn and sorghum in the SE of France. Adopting DSM with a relevant crop rotation and cover crop resulting in a better IWUE for this system compared with CT can effectively address water scarcity issues in this region. Copyright © 2011 John Wiley & Sons, Ltd.
Acquisition of MDR phenotype by leukemic cells is associated with increased caspase‐3 activity and a collateral sensitivity to cold stress
Authors: Cerezo David , Lencina Miriam , Ruiz‐Alcaraz Antonio J. , Ferragut José Antonio , Saceda Miguel , Sanchez Manuel , Cánovas Manuel , García‐Peñarrubia Pilar , Martín‐Orozco Elena 
Published: December 2011

AbstractThe acquisition of a multidrug‐resistant (MDR) phenotype by tumor cells that renders them unsusceptible to anti‐neoplasic agents is one of the main causes of chemotherapy failure in human malignancies. The increased expression of P‐glycoprotein (MDR1, P‐gp, ABCB1) in tumor cells contributes to drug resistance by extruding chemotherapeutic agents or by regulating programmed cell death. In a study of MDR cell survival under cold stress conditions, it was found that resistant leukemic cells with P‐gp over‐expression, but not their sensitive counterparts, are hypersensitive to cold‐induced cell death when exposed to temperatures below 4°C. The transfection of parental cells with a P‐gp‐expressing plasmid makes these cells sensitive to cold stress, demonstrating an association between P‐gp expression and cell death at low temperatures. Furthermore, we observed increased basal expression and activity of effector caspase‐3 at physiological temperature (37°C) in MDR cells compared with their parental cell line. Treatment with a caspase‐3 inhibitor partially rescues MDR leukemic cells from cold‐induced apoptosis, which suggests that the cell death mechanism may require caspase‐3 activity. Taken together, these findings demonstrate that P‐gp expression plays a role in MDR cell survival, and is accompanied by a collateral sensitivity to death induced by cold stress. These findings may assist in the design of specific therapeutic strategies to complement current chemotherapy treatment against cancer. J. Cell. Biochem. 113: 1416–1425, 2012. © 2011 Wiley Periodicals, Inc.
Continuously generated H2O2stimulates the proliferation and osteoblastic differentiation of human periodontal ligament fibroblasts
Authors: Choe Youngji , Yu Ji‐Yeon , Son Young‐Ok , Park Seung‐Moon , Kim Jong‐Ghee , Shi Xianglin , Lee Jeong‐Chae 
Published: December 2011

AbstractNumerous studies have shown that hydrogen peroxide (H2O2) inhibits proliferation and osteoblastic differentiation in bone‐like cells. Human periodontal ligament fibroblasts (PLF) are capable of differentiating into osteoblasts and are exposed to oxidative stress during periodontal inflammation. However, the cellular responses of PLF to H2O2have not been identified. In this study, we examined how H2O2affects the viability and proliferation of PLF by exposing the cells to glucose oxidase (GO) or direct addition of H2O2. We also explored the effects of GO on the osteoblastic differentiation of PLF and the mechanisms involved. The viability and proliferation in PLF were increased with the addition of 10 mU/ml GO but not by volumes greater than 15 mU/ml or by H2O2itself. GO‐stimulated DNA synthesis was correlated with the increase in cyclin E protein levels in the cells. Osteoblastic differentiation of PLF was also augmented by combined treatment with GO, as evidenced by the increases in alkaline phosphatase activity, mineralization, collagen synthesis, and osteocalcin content in the cells. The inductions of runt‐related transcription factor 2 and osterix mRNA and proteins were further increased in PLF incubated in combination with GO compared to those in untreated cells. These results demonstrate that the continuous presence of H2O2stimulates the proliferation of PLF and augments their potential to differentiate into osteoblasts through the up‐regulation of bone‐specific transcription factors. Collectively, we suggest that H2O2may elicit the functions of PLF in maintaining the dimensions of the periodontal ligament and in mediating a balanced metabolism in alveolar bone. J. Cell. Biochem. 113: 1426–1436, 2012. © 2011 Wiley Periodicals, Inc.
Small molecule induction of neural‐like cells from bone marrow‐mesenchymal stem cells
Authors: Wang Ye , He Wenfeng , Bian Hetao , Liu Chuan , Li Shiyong 
Published: December 2011

AbstractBone marrow‐derived mesenchymal stem cells (MSCs) have been demonstrated to be able to differentiate into neuron‐like cell, but the precise mechanisms controlling this process are unclear. We report here that LY294002, a small molecule inhibitor of PI3K/AKT signal pathway, can inhibit proliferation and promote neuronal differentiation of MSCs after MSCs incubated with LY294002 for 6 and 12 h. RT‐PCR results indicated that mRNA expression of α5β1 integrin significantly increased in neuron‐like cell from MSCs. Interestingly, neuron‐like cells derived by this method adhere much more strongly than MSCs, which was related to the expression of α5β1 integrin and FAK phosphorylation. However, these effects could be attenuated by LiCL or GSK‐3β–siRNA. Our results indicate that activation GSK‐3β signaling may be involved in MSCs proliferation, differentiation, and adhesion. Furthermore, this study demonstrates that small molecule regulators of PI3K/AKT signaling may be valuable tools for stem cell research aimed at treatment of neurodegenerative disease. J. Cell. Biochem. 113: 1527–1536, 2012. © 2011 Wiley Periodicals, Inc.
Hyperthermia‐enhanced TRAIL‐ and mapatumumab‐induced apoptotic death is mediated through mitochondria in human colon cancer cells
Authors: Song Xinxin , Kim Han‐Cheon , Kim Seog‐Young , Basse Per , Park Bae‐Hang , Lee Byeong‐Chel , Lee Yong J. 
Published: December 2011

AbstractColorectal cancer is the third leading cause of cancer‐related mortality in the world; death usually results from uncontrolled metastatic disease. Previously, we developed a novel strategy of TNF‐related apoptosis‐inducing ligand (Apo2L/TRAIL) in combination with hyperthermia to treat hepatic colorectal metastases. However, previous studies suggest a potential hepatocyte cytotoxicity with TRAIL. Unlike TRAIL, anti‐human TRAIL receptor antibody induces apoptosis without hepatocyte toxicity. In this study, we evaluated the anti‐tumor efficacy of humanized anti‐death receptor 4 (DR4) antibody mapatumumab (Mapa) by comparing it with TRAIL in combination with hyperthermia. TRAIL, which binds to both DR4 and death receptor 5 (DR5), was approximately tenfold more effective than Mapa in inducing apoptosis. However, hyperthermia enhances apoptosis induced by either agent. We observed that the synergistic effect was mediated through elevation of reactive oxygen species, c‐Jun N‐terminal kinase activation, Bax oligomerization, and translocalization to the mitochondria, loss of mitochondrial membrane potential, release of cytochrome c to cytosol, activation of caspases, and increase in poly(ADP‐ribose) polymerase cleavage. We believe that the successful outcome of this study will support the application of Mapa in combination with hyperthermia to colorectal hepatic metastases. J. Cell. Biochem. 113: 1547–1558, 2012. © 2011 Wiley Periodicals, Inc.
Macrophage migration inhibitory factor increases cell motility and up‐regulates αvβ3 integrin in human chondrosarcoma cells
Authors: Lee Chun‐Yi , Su Mei‐Ju , Huang Chun‐Yin , Chen Meng‐Yi , Hsu Horng‐Chaung , Lin Ching‐Yuang , Tang Chih‐Hsin 
Published: December 2011

AbstractThe macrophage migration‐inhibitory factor (MIF) is a pro‐inflammatory cytokine first known for its effect on macrophage migration and activation. Recent studies have shown that MIP plays a critical role in tumor growth, angiogenesis, and metastasis. Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. However, the effects of MIF on human chondrosarcoma cells are largely unknown. In the present study, MIF was found to increase the migration and the expression of αvβ3 integrin in human chondrosarcoma cells. The phosphatidylinositol 3‐kinase (PI3K), Akt, and NF‐κB pathways were activated by MIF treatment, and the MIF‐induced expression of integrin and migration activity were inhibited by the specific inhibitors and mutant forms of PI3K, Akt, and NF‐κB cascades. In addition, migration‐prone sublines demonstrated that increased cell migration ability was correlated with increased expression of MIF and αvβ3 integrin. Taken together, our results indicate that MIF enhanced the migration of the chondrosarcoma cells by increasing αvβ3 integrin expression through the PI3K/Akt/NF‐κB signal transduction pathway. J. Cell. Biochem. 113: 1590–1598, 2012. © 2011 Wiley Periodicals, Inc.
Effect of phorbol 12‐myristate 13‐acetate activated signaling pathways on 1α, 25 dihydroxyvitamin D3 Regulated Human 25‐hydroxyvitamin D3 24‐hydroxylase Gene Expression in Differentiated Caco‐2 Cells
Authors: Jiang Yan , Fleet James C. 
Published: December 2011

AbstractPhorbol‐12‐myristate‐13‐acetate (PMA), a protein kinase C (PKC) activator, can modulate 1α, 25 dihydroxyvitamin D3 (1,25(OH)2D3)‐induced expression of the 24‐hydroxylase (CYP24A1) gene but this has not been studied in differentiated enterocytes, a primary 1,25(OH)2D3 target cell. We found that in differentiated Caco‐2 cells, an established model of the mature absorptive epithelial cell, PMA significantly enhanced 1,25(OH)2D3‐induced human CYP24A1 (hCYP24A1) mRNA accumulation and hCYP24A1 promoter‐luciferase reporter gene activation by 150%. Reporter gene studies further identified the region between −298 and +74 bp in the hCYP24A1 promoter as critical for the PMA enhancing effect and chromatin immunoprecipitation (ChIP) analysis showed that PMA enhanced 1,25(OH)2D3‐induced binding of vitamin D receptor to this region. PMA can activate PKC, ERK1/2, and p38 MAP kinases and inhibition of these signaling pathways reduced both 1,25(OH)2D3‐induced hCYP24A1 gene transcription and the enhancing effect of PMA. The PMA enhancing effect on 1,25(OH)2D3 action was evident in a minimal promoter with three osteocalcin VDREs and was reduced after mutation of a putative vitamin D stimulatory site in the hCYP24A1 promoter. In contrast, mutation of a Ets binding site (EBS) in the hCYP24A1 promoter had no impact on 1,25(OH)2D3 action or the PMA enhancing effect. These data suggest that in the differentiated enterocyte PMA‐induced activation of several signaling pathways contribute to 1,25(OH)2D3‐induced hCYP24A1 gene expression through multiple regulatory motifs within the proximal hCYP24A1 promoter. J. Cell. Biochem. 113: 1599–1607, 2012. © 2011 Wiley Periodicals, Inc.
Nonmedical factors associated with prolonged hospital length of stay in an urban homebound population
Authors: Foer Dinah , Ornstein Katherine , Soriano Theresa A. , Kathuria Navneet , Dunn Andrew 
Published: December 2011

AbstractBACKGROUND:Prolonged length of stay (LOS) is a major concern for hospitalized populations at risk for adverse events. Homebound patients are at particular risk for long stays and may have unique discharge needs because of their commitment to be cared for at home despite poor functional status.OBJECTIVE:The goal of this study was to describe factors contributing to long hospitalizations in the homebound population.DESIGN:This retrospective observational pilot study included all 2007 discharges that occurred for patients at The Mount Sinai Hospital enrolled in the Mount Sinai Visiting Doctors Program.MEASURES:Long‐stay patients were defined as those having an LOS 2 standard deviations above the mean. Hospitalization days were defined as “nonmedical” when patients medically ready for discharge remained in the hospital. Patients discharged immediately after determination of medical readiness were characterized as “medical stay” cases. The University HealthSystems Consortium Database was used to calculate expected LOS and the LOS ratio. Chart reviews were performed to describe long‐stay cases as nonmedical or medical.RESULTS:The average LOS for 479 discharges was 7.84 days, with a mean LOS Ratio of 1.23. Seventeen cases were determined to be long stays. Eight of these cases (47%) were defined as nonmedical stays. These accounted for 136 days of hospitalization and 32% of total long‐stay days. The most common reason for a nonmedical stay was nursing facility placement delay.CONCLUSIONS:Nonmedical factors accounted for nearly one‐third of all long‐stay days in the hospitalized homebound population. Increased interdisciplinary collaboration may help address homebound patient LOS. Journal of Hospital Medicine 2012; © 2012 Society of Hospital Medicine.
Predictive value of MRI in the localization, staging, volume estimation, assessment of aggressiveness, and guidance of radiotherapy and biopsies in prostate cancer
Authors: Yakar Derya , Debats Oscar A. , Bomers Joyce G.R. , Schouten Martijn G. , Vos Pieter C. , van Lin Emile , Fütterer Jurgen J. , Barentsz Jelle O. 
Published: December 2011

AbstractMultiparametric magnetic resonance imaging (MRI) has the potential of being the ideal prostate cancer (PCa) assessment tool. Information gathered with multiparametric MRI can serve therapy choice, guidance of interventions, and treatments. The purpose of this review is to discuss the potential role of multiparametric MRI in focal therapy with respect to patient selection and directing (robot‐guided) biopsies and intensity‐modulated radiation therapy (IMRT). Multiparametric MRI is a versatile and promising technique. It appears to be the best available imaging technique at the moment in localizing, staging (primary as well as recurrent disease, and local as well as distant disease), determining aggressiveness, and volume of PCa. However, larger study populations in multicenter settings have to confirm these promising results. However, before such studies can be performed more research is needed in order to achieve standardized imaging protocols. J. Magn. Reson. Imaging 2012;35:20‐31. © 2011 Wiley Periodicals, Inc.
Non‐contrast enhanced MR angiography: Established techniques
Authors: Miyazaki Mitsue , Akahane Masaaki 
Published: December 2011

AbstractUntil recently, time‐of‐flight (TOF) and phase contrast (PC) were the only non‐contrast MR angiography (NC‐MRA) techniques practically used in clinical. In the decade, NC‐MRA have been gained a revival of an interest among the MR researchers and scientists, in part because of safety concerns related to the possible link between gadolinium‐based contrast agents and nephrogenic systemic fibrosis (NSF). This article introduces other established NC‐MRA techniques, such as ECG‐gated partial Fourier fast spin echo (FSE) and balanced steady‐state free precession (bSSFP), both with and without arterial spin labeling. Then, the article focuses on two main applications: peripheral run‐off and renal MRA. Recently, both applications have achieved remarkable advancements and have become a viable clinical option as an alternative to contrast‐enhanced (CE)‐MRA. In addition, developments on the horizon including whole body MRA applications and further advancement at 3 Tesla are discussed. J. Magn. Reson. Imaging 2012 © 2011 Wiley Periodicals, Inc.
Pooling health‐related quality of life outcomes in meta‐analysis—a tutorial and review of methods for enhancing interpretability
Authors: Thorlund Kristian , Walter Stephen D. , Johnston Bradley C. , Furukawa Toshi A. , Guyatt Gordon H. 
Published: December 2011

Background– Meta‐analyses of health‐related quality of life (HRQL) outcomes present difficulties in interpretation when studies use different instruments to measure the same construct. Presentation of results in standard deviation units (standardized mean difference) is widely used but is limited by vulnerability to differential variability in populations enrolled and interpretational challenges.Objective– The objective of this study is to identify and describe the available approaches for enhancing interpretability of meta‐analyses involving HRQL outcomes.Findings– We identified 12 approaches in three categories:

Summary estimates derived from the pooled standardized mean difference: conversion to units of the most familiar instrument or to risk difference or odds ratio. These approaches remain vulnerable to differential variability in populations.
Summary estimates derived from the individual trial summary statistics: conversion to units of the most familiar instrument or to ratio of means. Both are appropriate complementary approaches to measures derived from converted probabilities.
Summary estimates derived from the individual trial summary statistics and established minimally important differences for all instruments: presentation in minimally important difference units or conversion to risk difference or odds ratio. Risk differences are ideal for balancing desirable and undesirable consequences of alternative interventions.Conclusion– The use of these approaches may enhance the interpretability and the usefulness of systematic reviews involving HRQL outcomes. Copyright © 2011 John Wiley & Sons, Ltd.
Boundedness of solutions of retarded functional differential equations with variable impulses via generalized ordinary differential equations
Authors: Afonso S. M. , Bonotto E. M. , Federson M. , Gimenes L. P. 
Published: December 2011

AbstractIn this paper, we give sufficient conditions for the uniform boundedness and uniform ultimate boundedness of solutions of a class of retarded functional differential equations with impulse effects acting on variable times. We employ the theory of generalized ordinary differential equations to obtain our results. As an example, we investigate the boundedness of the solution of a circulating fuel nuclear reactor model.
Synthesis of High Molecular Weight Polyesters via In Vacuo Dehydrogenation Polymerization of Diols
Authors: Hunsicker David M. , Dauphinais Brian C. , Mc Ilrath Sean P. , Robertson Nicholas J. 
Published: December 2011

AbstractThe Milstein catalyst has proven to be highly effective for the conversion of alcohols to esters, as well as alcohols and amines to amides and polyamides. We have recently found that the catalyst's range can be extended to very efficient in vacuo dehydrogenation polymerization of α,ω‐diols to generate polyesters. The gaseous hydrogen byproduct that is produced is easily removed to drive the equilibrium toward product, which leads to the formation of high molecular weight polymer ( up to 145 000 g mol−1). This optimized methodology works well to polymerize diols with a spacer of six carbons or more. Diols with fewer carbons are cyclized to lactone; the dividing point is the dehydrogenation of 1,5‐pentanediol, which leads to a mixture of polyester and lactone. Reported herein is the synthesis and characterization of five aliphatic polyesters prepared via this novel dehydrogenation polymerization approach.
Model to Design Multilayer Tissue Engineering Scaffolds
Authors: Papenburg Bernke J. , Higuera Gustavo A. , de Vries Ida‐Jacoba , de Boer Jan , van Blitterswijk Clemens A. , Wessling Matthias , Stamatialis Dimitrios 
Published: December 2011

AbstractIn tissue engineering scaffolds, nutrient transport to cells is a major hurdle for building viable 3D tissue scaffolds.In vivo, a complex network of blood vessels and capillaries delivers nutrients and oxygen to the tissue. Forin vitrobuild constructs the distance to the main nutrient source is often long resulting in nutrient gradients over the scaffold and decreased cell proliferation or even cell death in parts of the scaffold. This work describes a numerical model for designing a multilayer scaffold for tissue engineering by stacking sheets featuring microchannels. These sheets fabricated by phase separation micromolding (PSµM) allow nutrient perfusion through the 3D scaffold whereas inner‐porosity of the sheets guarantees diffusion between layers. The model predicts significant nutrient limitation occurs under static conditions for scaffolds having over 3 sheets. The situation can improve dramatically under dynamic conditions when perfusion occurs through the microchannels.
Injectable Hydrogels by Enzymatic Co‐Crosslinking of Dextran and Hyaluronic Acid Tyramine Conjugates
Authors: Wennink Jos W.H. , Niederer Kerstin , Bochyńska Agnieszka I. , Moreira Teixeira Liliana S. , Karperien Marcel , Feijen Jan , Dijkstra Pieter J. 
Published: December 2011

AbstractIn this study, injectable hydrogels were prepared by the horseradish peroxidase mediated co‐crosslinking of dextran‐tyramine (Dex‐TA) and hyaluronic acid‐tyramine (HA‐TA) conjugates intended for cartilage tissue engineering. In general the gelation times of 10 wt% polysaccharide solutions are < 20 seconds and their storage moduli can be adjusted by varying the composition between Dex‐TA and HA‐TA. Dex‐TA/HA‐TA (50/50) hydrogels were fully degradable in the presence of hyaluronidase. Chondrocytes incorporated in 10 wt% Dex‐TA and Dex‐TA/HA‐TA (50/50) gels showed good viability after 28 days. These results indicate that Dex‐TA/HA‐TA (50/50) hydrogels are promising injectable and biodegradable hydrogels for cartilage repair.
Thermo‐Reversible Reactions for the Preparation of Smart Materials: Recyclable Covalently‐Crosslinked Shape Memory Polymers
Authors: Defize Thomas , Riva Raphaël , Thomassin Jean‐Michel , Jérôme Christine , Alexandre Michaël 
Published: December 2011

Abstract[4+2] thermoreversible Diels‐Alder cycloaddition has been used to crosslink star‐shaped poly(ε‐caprolactone) in order to produce networks based on strong carbon‐carbon covalent bondings. Depending on the nature of the Diels‐Alder reactants, these bonds can be thermoreversibly broken, allowing re‐processing of the polymer matrix.