bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED: Fibroblast growth factor‐2 promotes catabolism via FGFR1‐Ras‐Raf‐MEK1/2‐ERK1/2 axis that coordinates with the PKCδ pathway in human articular chondrocytes
Authors: Yan Dongyao , Chen Di , Im Hee‐Jeong 
Published: April 2012

AbstractFibroblast growth factor 2 (FGF‐2) has been found to play an anti‐anabolic and/or a catabolic role in adult human articular cartilage via regulation of multiple signaling pathways. Upon FGF‐2 stimulation, a molecular crosstalk between the mitogen activated protein kinase (MAPK) and protein kinase C δ (PKCδ) pathways are initiated, where PKCδ positively regulates downstream MAPK signaling. In this study, we explored the relationship between fibroblast growth factor receptor 1 (FGFR1), Ras, and PKCδ in FGF‐2 signaling in human articular chondrocytes. Pathway‐specific inhibition using both chemical inhibitors and siRNA targeting FGFR1 demonstrated that, upon FGF‐2 stimulation, FGFR1 controlled both Ras and PKCδ activation, which converged on the Raf‐MEK1/2‐ERK1/2 axis. No crosstalk was observed between Ras and PKCδ. Quantitative PCR analyses revealed that both Ras and PKCδ contributed to FGF‐2‐mediated upregulation of MMP‐13, ADAMTS5, and repression of aggrecan gene. Correspondingly, FGF‐2‐mediated proteoglycan loss was effectively reversed by individual pathway‐specific inhibitor of Ras, PKCδ, and ERK1/2 in both 3‐dimensional alginate bead culture and cartilage organ culture systems. Our findings suggest that FGFR1 interacts with FGF‐2 and then activates Ras and PKCδ, which concertedly drive MAPK signaling to mediate biological effects of FGF‐2. Such an integration of dual inputs constitutes a novel mechanism of FGF‐2 signaling cascade in human articular chondrocytes. J. Cell. Biochem. 113: 2856–2865, 2012. © 2012 Wiley Periodicals, Inc.
Asymmetrical distribution of antennal sensilla in the female Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae)
Authors: Meng Zhao Jun , Yan Shan Chun , Yang Chuan Ping , Ruan Chang Chun 
Published: April 2012

AbstractAnastatus japonicus Ashmead (Hymenoptera: Eupelmidae) is an important egg parasitoid of several major insect pests. To better understand its host finding mechanisms, the antennal sensilla of female wasp were investigated by scanning electron microscopy. Sensilla chaetica were found mainly on radicle and pedicel segments of the antennae. i‐Type sensilla, s. campaniformia, and corneous sensilla were detected on the leeward side, while s. coeloconica and lance sensilla were presented on the windward side of the antennae. S. trichodea and s. basiconica were more abundant on the leeward side than on the windward side of the antennae. More s. placodea were found on the windward side than on the leeward side of the right antenna, while the opposite results were observed on the left antenna. Overall, more s. placodea were found on the right antenna than that on the left antenna. The numbers of s. trichodea and s. basiconica on the clava or the third flagellum antennomere of the right antenna were more than those of the left antenna, whereas their distribution patterns on the other corresponding antennomeres were reverse. Our results showed that there is a strong asymmetrical antennal sensilla distribution quantitatively and spatially between the left and right antennae. Placoid sensilla are present more on the right antenna than on the left antenna. S. campaniformia, corneous sensilla, and i‐type sensilla were found only on the leeward side of the antennal clava, while their external morphology and potential functions were described and discussed in detail for the first time. Microsc. Res. Tech. 75:1066–1075, 2012. © 2012 Wiley Periodicals, Inc.
Partition coefficients for gadolinium chelates in the normal myocardium: Comparison of gadopentetate dimeglumine and gadobenate dimeglumine
Authors: Kawel Nadine , Nacif Marcelo , Santini Francesco , Liu Songtao , Bremerich Jens , Arai Andrew E. , Bluemke David A. 
Published: April 2012

AbstractPurpose:To evaluate the influence of contrast agents with different relaxivity on the partition coefficient (λ) and timing of equilibration using a modified Look‐Locker inversion recovery (MOLLI) sequence in cardiac magnetic resonance imaging (MRI).Materials and Methods:MOLLI was acquired in 20 healthy subjects (1.5T) at the mid‐ventricular short axis precontrast and 5, 10, 20, 25, and 30 minutes after administration of a bolus of 0.15 mmol/kg gadobenate dimeglumine (Gd‐BOPTA) (n = 10) or gadopentetate dimeglumine (Gd‐DTPA) (n = 10). T1 times were measured in myocardium and blood pool. λ was approximated by ΔR1myocardium/ΔR1blood. Values for Gd‐BOPTA and Gd‐DTPA were compared. Interobserver agreement was evaluated (intraclass correlation coefficient [ICC]).Results:T1 times of myocardium and blood pool (P < 0.001) and λ (0.42 ± 0.03 and 0.47 ± 0.04, respectively, P < 0.001; excluding 5 minutes for Gd‐BOPTA) were significantly lower for Gd‐BOPTA than Gd‐DTPA. The λ(Gd‐DTPA) showed no significant variation between 5 and 30 minutes. The λ(Gd‐BOPTA) values were significantly lower at 5 minutes compared to other times (0.38 vs. 0.42; P < 0.05). Interobserver agreement for λ values was excellent with Gd‐BOPTA (ICC = 0.818) and good for Gd‐DTPA (ICC = 0.631).Conclusion:The λ(Gd‐BOPTA) values were significantly lower compared to λ(Gd‐DTPA) at the same administered dose. Using Gd‐BOPTA, the equilibrium between myocardium and blood pool was not achieved at 5 minutes postcontrast. J. Magn. Reson. Imaging 2012;36:733–737. © 2012 Wiley Periodicals, Inc.
Methylmalonate impairs mitochondrial respiration supported by NADH‐linked substrates: Involvement of mitochondrial glutamate metabolism
Authors: Melo Daniela R. , Mirandola Sandra R. , Assunção Nilson A. , Castilho Roger F. 
Published: April 2012

AbstractThe neurodegeneration that occurs in methylmalonic acidemia is proposed to be associated with impairment of mitochondrial oxidative metabolism resulting from methylmalonate (MMA) accumulation. The present study evaluated the effects of MMA on oxygen consumption by isolated rat brain mitochondria in the presence of NADH‐linked substrates (α‐ketoglutarate, citrate, isocitrate, glutamate, malate, and pyruvate). Respiration supported either by glutamate or glutamate plus malate was significantly inhibited by MMA (1–10 mM), whereas no inhibition was observed when a cocktail of NADH‐linked substrates was used. Measurements of glutamate transport revealed that the inhibitory effect of MMA on respiration maintained by this substrate is not due to inhibition of its mitochondrial uptake. In light of this result, the effect of MMA on the activity of relevant enzymes involved in mitochondrial glutamate metabolism was investigated. MMA had minor inhibitory effects on glutamate dehydrogenase and aspartate aminotransferase, whereas α‐ketoglutarate dehydrogenase was significantly inhibited by this metabolite (Ki = 3.65 mM). Moreover, measurements of α‐ketoglutarate transport and mitochondrial MMA accumulation indicated that MMA/α‐ketoglutarate exchange depletes mitochondria from this substrate, which may further contribute to the inhibition of glutamate‐sustained respiration. To study the effect of chronic in vivo MMA treatment on mitochondrial function, young rats were intraperitoneally injected with MMA. No significant difference was observed in respiration between isolated brain mitochondria from control and MMA‐treated rats, indicating that in vivo MMA treatment did not lead to permanent mitochondrial respiratory defects. Taken together, these findings indicate that the inhibitory effect of MMA on mitochondrial oxidative metabolism can be ascribed to concurrent inhibition of specific enzymes and lower availability of respiratory substrates. © 2012 Wiley Periodicals, Inc.
Analysis of rainfall and temperature trends in northeast India
Authors: Jain S. K. , Kumar Vijay , Saharia M. 
Published: April 2012

AbstractThe northeast region (NER) of India covers an area of 0.26 million km2. This region is one of the highest rainfall‐receiving regions on the planet. Consequently, it has huge water and hydropower potential and analysis of rainfall and temperature trends would be of interest to water and energy planners. Trends in monthly, seasonal, and annual rainfall and temperature on the subdivision and regional scale for the NER were examined in this study. Trend analysis of rainfall data series for 1871–2008 did not show any clear trend for the region as a whole, although there are seasonal trends for some seasons and for some hydro‐meteorological subdivisions. Similar analysis for temperature data showed that all the four temperature variables (maximum, minimum, and mean temperatures and temperature range) had rising trend. Notably for the post‐monsoon season, the Sen's estimator of slope ( °C/year) was 0.019, 0.011, and 0.015 for the maximum, minimum, and mean temperature, respectively. Copyright © 2012 Royal Meteorological Society
Is double inactivation of the Nf1 gene responsible for the development of congenital pseudarthrosis of the tibia associated with NF1?
Authors: Lee Sang Min , Choi In Ho , Lee Dong Yeon , Lee Hye Ran , Park Moon Seok , Yoo Won Joon , Chung Chin Youb , Cho Tae‐Joon 
Published: April 2012

AbstractThe pathogenic mechanism responsible for congenital pseudarthrosis of the tibia (CPT) is not well understood although the possibility of double inactivation of the neurofibromatosis type 1 (Nf1) gene has been suggested. In the present study, loss of heterozygosity was investigated in fibrous hamartoma tissues harvested from 16 patients with CPT associated with NF1 using four genetic markers that span the Nf1 gene. Based on the assumption that a single cell with double inactivation of Nf1 would undergo clonal growth and cause fibrous hamartoma, we investigated clonality in fibrous hamartoma tissues by analyzing X‐chromosome inactivation patterns in 11 female patients. Loss of Nf1 heterozygosity in fibrous hamartoma tissues was observed at one or two genetic markers in 4 out of the 16 patients tested. In clonality assays, 3 of 11 patients showed a clonal growth pattern, 5 a non‐clonal pattern, and 3 were non‐informative. These findings support that double inactivation of the Nf1 gene and subsequent clonal growth could be a pathogenic feature of the fibrous hamartoma tissue at least in some of the CPT but might not be essential requirements of CPT development. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1535–1540, 2012
Healthy food from organic wheat: choice of genotypes for production and breeding
Authors: Hussain Abrar , Larsson Hans , Kuktaite Ramune , Johansson Eva 
Published: April 2012

AbstractBACKGROUND: In the present study, 40 wheat genotypes were grown in the same soil in organic farming system trials in Alnarp, Sweden. The purpose was to evaluate opportunities for production and breeding of organic wheat of high nutritious value.RESULTS: The results showed a large variation in content of minerals, total tocochromanols and heavy metals in the grain of 40 organically produced wheat genotypes. Principal component and cluster analysis were used as tools for selection of the most suitable genotypes for production and breeding of organic wheat of high nutritious value. No single genotype group was found particularly superior from the studied material to produce this specific type of wheat. However, certain genotypes from different groups were found with promising nutritional characters. The most promising genotypes as related to nutritionally relevant compounds were 6356 spelt, Triticum monococcum, Ölands 17 borst spelt, Lv Dal 16 brun borst and Fylgia.CONCLUSION: By choosing these genotypes for organic production and future wheat breeding, nutritionally improved organic wheat products might be developed. However, for future breeding, nutritional components such as protein, fibre, glycaemic index and B‐group vitamins should also be considered. Copyright © 2012 Society of Chemical Industry
Influence of habitat features and hunter behavior on white‐tailed deer harvest
Authors: Lebel François , Dussault Christian , Massé Ariane , Côté Steeve D. 
Published: April 2012

AbstractSport hunting may help in controlling cervid populations over large areas. As with natural predators, several environmental factors can influence sport harvest. A better understanding of the environmental variables that limit the efficiency of sport hunting could provide guidelines for more efficient wildlife management using hunting. We studied white‐tailed deer (Odocoileus virginianus) hunting on a high deer density island where hunting was the sole form of predation. Our objective was to study the behavior of sport hunters and determine the habitat characteristics (e.g., abundance of deer forage, visibility of the deer from the hunter's point of view, and accessibility of the territory to hunters) that are associated with a successful harvest. We collected movements and harvest site location data from 477 hunters equipped with handheld Global Positioning System (GPS) units. Harvest sites were visited and characterized, along with a paired random site, to determine the environmental conditions associated with a successful hunt. We also developed a model to predict the daily number of deer seen by hunters considering weather conditions, hunter characteristics (e.g., age, experience), and date of hunting. We used the mean number of deer seen per hunter per day as a relative index of local density in each hunted territory. At both the site and landscape scales, the combination of visibility and access had a positive effect on the distribution of harvested deer. Habitat types with less visual obstruction from vegetation enabled hunters to see more deer in a given day. At the site scale, harvested deer were located in areas with a lower density of access routes compared to areas where hunters travelled throughout the day. Using an innovative approach of studying hunter behavior with GPS technology, digital maps, and questionnaires, we highlighted the factors associated with hunter success. Our study suggests that habitat characteristics could be modified to increase harvest by improving accessibility and visibility near roads. Creating openings in mature and regenerating forest near access roads could make sport hunting a more efficient management tool, but the potential impact of increased forage availability in forest openings should not be overlooked. © 2012 The Wildlife Society.
A comparison of methods for estimating raccoon abundance: Implications for disease vaccination programs
Authors: Beasley James C. , Beatty William S. , Atwood Todd C. , Johnson Shylo R. , Rhodes Olin E. 
Published: April 2012

AbstractAccurate estimates of demographic parameters are critical to the management of wildlife populations, including management programs focused on controlling the spread of zoonotic diseases. Rabies managers in the United States Department of Agriculture (USDA) have applied a simple raccoon (Procyon lotor) abundance index (RAI) based on cumulative catch of unique raccoons per unit area to determine vaccine‐bait distribution densities. This approach was designed to allow for both the collection of biological samples and to index raccoon abundance to determine bait densities for oral rabies programs. However, post‐baiting surveillance data indicate that, on average, only 30% of raccoons sampled have vaccine induced rabies antibody titers, suggesting that bait densities may not be well calibrated to raccoon densities. We trapped raccoons using both capture‐mark‐recapture (CMR) and the standard RAI to evaluate the accuracy of the current index‐based methodology for estimating raccoon density. We then developed a resource selection function from spatial data collected from radio‐collared raccoons to standardize trap placement within the existing RAI protocol, and evaluated the performance of this modified RAI approach relative to CMR for estimating raccoon population size. Both abundance and density estimates derived using the RAI consistently underestimated raccoon population sizes compared with CMR methods. Similarly, although the use of resource selection models to inform trap placement appeared to improve the accuracy of the RAI, the effectiveness of this method was inconsistent because of an inability to account for variance in detection probabilities. Despite the logistical advantages of using indices to estimate population parameters to determine vaccine bait distribution densities, our results suggest that adjustments may be necessary to more accurately quantify raccoon abundance, which should improve the effectiveness of rabies management in the United States. In particular, estimates of detection probabilities are needed to more precisely quantify abundance estimates and ensure appropriate vaccine coverage rates. © 2012 The Wildlife Society.
Wound botulism presenting as a deep neck space infection
Authors: Gouveia Christopher , Mookherjee Somnath , Russell Matthew S. 
Published: April 2012

AbstractOtolaryngologists commonly evaluate patients with findings suspicious for deep space soft tissue infections of the neck. In this case, a woman with a history of injection drug use (IDU) presented with dysphagia, odynophagia, and neck pain. Multiple neck abscesses, too small to drain, were seen on imaging. Despite broad‐spectrum intravenous antibiotics, she unexpectedly and rapidly developed respiratory failure requiring intubation. Further work‐up diagnosed wound botulism (WB). To our knowledge, this is the first report of WB presenting as a deep neck space infection, and illustrates the importance of considering this deadly diagnosis in patients with IDU history and bulbar symptoms.
Prevertebral tendinitis: How to avoid unnecessary surgical interventions
Authors: Hammer Georg Philipp , Vollmann Robert , Tomazic Peter Valentin , Simbrunner Josef , Friedrich Gerhard 
Published: April 2012

AbstractObjectives/Hypothesis:Prevertebral tendinitis is an inflammatory process that affects the cervicothoracic prevertebral muscles. Because of its clinical presentation and imaging features in computed tomography scans, prevertebral tendinitis can easily be mistaken for deep cervical abscess formation. Totally different therapy regimens require clinical and diagnostic pathways for sufficient differentiation between those two pathologic entities.Study Design:Case series with comparison.Methods:In 10 patients with prevertebral tendinitis, we evaluated the symptoms, laboratory reports, and radiological imaging findings. We compared these data to 65 patients with a deep cervical abscess formation. The basic radiologic imaging procedure was contrast‐enhanced computed tomography. For detection of prevertebral tendinitis, we performed magnetic resonance imaging with diffusion‐weighted images and calculated the apparent diffusion coefficient map.Results:Patients with prevertebral tendinitis complained of severe neck pain, globus sensation, and neck stiffness. Diffusion‐weighted images showed a typical benign prevertebral effusion. Computed tomography scans showed amorphous calcifications in the tendon of the prevertebral muscles. The C‐reactive protein values were slightly increased in patients with prevertebral tendinitis, and white blood cell count remained normal. In comparison to patients with deep cervical abscess formation, the C‐reactive protein and white blood cell count was significantly lower (P < .05) in the prevertebral tendinitis cases.Conclusions:Prevertebral tendinitis should be considered when patients suffer from neck pain, neck stiffness, and globus sensations despite low signs of inflammation in the laboratory report. To confirm the diagnosis, the best imaging feature is magnetic resonance imaging with diffusion‐weighted images and apparent diffusion coefficient map. Laryngoscope, 2012
Temporal patterns of head and neck squamous cell carcinoma recurrence with positron‐emission tomography/computed tomography monitoring
Authors: Beswick Daniel M. , Gooding William E. , Johnson Jonas T. , Branstetter Barton F. 
Published: April 2012

AbstractObjectives/Hypothesis:To estimate the distribution of head and neck squamous cell carcinoma (HNSCC) recurrence after definitive chemoradiation therapy (CRT) among patients who underwent 18F‐fluorodeoxyglucose positron‐emission tomography and computed tomography (PET/CT) surveillance.Study Design:Retrospective review.Methods:HNSCC patients who underwent definitive CRT from 2001 to 2008 were evaluated for recurrence with serial PET/CT. Patients were excluded if they were previously treated for recurrent disease, were treated with surgery as the primary therapeutic modality, or had inadequate clinical follow‐up. Recurrence was defined by histopathologic evidence of tumor.Results:Three hundred eighty‐eight patients were studied. Patients in whom recurrence was not detected were followed clinically and radiographically for a median of 27 months. Tumor recurrence was detected in 110 patients. For 37 patients, recurrence was heralded by clinical signs. Among the 73 asymptomatic patients who had a confirmed recurrence, disease was detected by PET/CT between 2 and 43 months, median of 6 months. Forty‐five percent of observed asymptomatic recurrences were detected during the first 6 months of surveillance (95% confidence interval [CI], 34%‐57%), 79% within the first 12 months (95% CI, 68%‐88%), 95% within the first 24 months (95% CI, 87%‐98%), and 100% within the first 48 months (95% CI, 95%‐100%).Conclusions:Among HNSCC patients followed with PET/CT surveillance, 95% of observed asymptomatic recurrences were detected within 24 months after completing CRT. For patients without clinical signs of recurrence, routine PET/CT surveillance beyond the first 24 months may be of limited value and may not be cost effective. Laryngoscope, 2012
What is “Success” following surgery for obstructive sleep apnea? The effect of different polysomnographic scoring systems
Authors: Hobson Jonathan C. , Robinson Sam , Antic Nick A. , McEvoy R. Doug , Windler Samantha , MacKay Stuart , Carney A. Simon 
Published: April 2012

AbstractObjectives/Hypothesis:To illustrate that the diagnosis of obstructive sleep apnea (OSA) is dependent on the polysomnographic scoring criteria used, and the success rates of treatments for OSA are dependent on the defined outcome measures.Study Design:Retrospective case series with prospective reanalysis of polysomnographic data.Methods:Consecutively treated adult patients (N = 40) with moderate to severe OSA having multilevel pharyngeal surgery in 2007 were studied. All patients underwent submucosal lingualplasty and concurrent or previous uvulopalatopharyngoplasty ± palatal advancement. Full polysomnography (PSG) was performed preoperatively and at a mean of 145 days postoperatively. Pre‐ and postoperative PSG data were analyzed by two different but widely used scoring systems for the apnea‐hypopnea index (AHI): The American Academy of Sleep Medicine (AASM) 1999 Chicago criteria and the AASM 2007 recommended criteria.Results:Follow‐up PSG data were available in 31 of 40 patients. Successful surgery was defined as a reduction in AHIRec <20 with a 50% reduction from the patient's baseline, and in this group the surgical intervention was associated with a 72.2% success rate. If, however, differing AHI metrics are used or the absolute or percent reduction used to define a successful outcome is changed, then the rate of surgical success is shown to range from 39% to 92%.Conclusions:Different criteria for measuring AHI and defining success following OSA surgery can produce widely conflicting outcome data. Reported results following OSA surgery should be interpreted with this in mind. Using acceptable criteria, multilevel sleep surgery can be demonstrated to be of benefit to the majority of carefully selected patients. Laryngoscope, 2012