bluuebunny/crossref_metadata_2025_split huggingface dataset:
A dual-inducible control system for multistep biosynthetic pathways
Authors: Rincón Andrés Felipe Carrillo , Cabral Alexandra J. , Gyorgy Andras , Farny Natalie G. 
Published: June 2024

ABSTRACTBackgroundThe successful production of industrially relevant natural products hinges on two key factors: the cultivation of robust microbial chassis capable of synthesizing the desired compounds, and the availability of reliable genetic tools for expressing target genes. The development of versatile and portable genetic tools offers a streamlined pathway to efficiently produce a variety of compounds in well-established chassis organisms. The σ70lacandtetexpression systems – adaptations of the widely usedlacandtetregulatory systems developed in our laboratory – have shown effective regulation and robust expression of recombinant proteins in various Gram-negative bacteria. Understanding the strengths and limitations of these regulatory systems in controlling recombinant protein production is essential for progress in this area.ResultsTo assess their capacity for combinatorial control, both the σ70lacandtetexpression systems were combined into a single plasmid and assessed for their performance in producing fluorescent reporters as well as the terpenoids lycopene and β-carotene. We thoroughly characterized the induction range, potential for synergistic effects, and metabolic costs of our dual σ70lacandtetexpression system in the well-established microorganismsEscherichia coli,Pseudomonas putida, andVibrio natriegensusing combinations of fluorescent reporters. The dynamic range and basal transcriptional control of the σ70expression systems were further improved through the incorporation of translational control mechanisms via toehold switches. This improvement was assessed using the highly sensitive luciferase reporter system. The improvement in control afforded by the integration of the toehold switches enabled the accumulation of a biosynthetic intermediate (lycopene) in the β-carotene synthesis pathway.ConclusionThis study presents the development and remaining challenges of a set of versatile genetic tools that are portable across well-established gammaproteobacterial chassis and capable of controlling the expression of multigene biosynthetic pathways. The enhanced σ70expression systems, combined with toehold switches, facilitate the biosynthesis and study of enzymes, recombinant proteins, and natural products, thus providing a valuable resource for producing a variety of compounds in microbial cell factories.GRAPHICAL ABSTRACT
Nub1 traps unfolded FAT10 for ubiquitin-independent degradation by the 26S proteasome
Authors: Arkinson Connor , Dong Ken C. , Gee Christine L. , Costello Shawn M. , Marqusee Susan , Martin Andreas 
Published: June 2024

SummaryThe ubiquitin-like modifier FAT10 targets hundreds of proteins in the mammalian immune system to the 26S proteasome for degradation. This degradation pathway requires the cofactor Nub1, yet the underlying mechanisms remain unknown. Here, we reconstituted a minimalin vitrosystem and revealed that Nub1 utilizes FAT10’s intrinsic instability to trap its N-terminal ubiquitin-like domain in an unfolded state and deliver it to the 26S proteasome for engagement, allowing the degradation of FAT10-ylated substrates in a ubiquitin– and p97-independent manner. Through hydrogen-deuterium exchange, structural modeling, and site-directed mutagenesis, we identified the formation of a peculiar complex with FAT10 that activates Nub1 for docking to the 26S proteasome, and our cryo-EM studies visualized the highly dynamic Nub1 complex bound to the proteasomal Rpn1 subunit during FAT10 delivery and the early stages of ATP-dependent degradation. These studies thus identified a novel mode of cofactor-mediated, ubiquitin-independent substrate delivery to the 26S proteasome that relies on trapping partially unfolded states for engagement by the proteasomal ATPase motor.
Stage 2a IDEAL evaluation of a third-generation biocomposite suture anchor in arthroscopic rotator cuff repair: Subgroup cohort analysis of the PRULO registry with 12-month follow up
Authors: Scholes Corey , Fatima Manaal , Moody Cooper , Eng Kevin , Page Richard S 
Published: June 2024

AbstractBackgroundPoly-lactic co-glycolide with tricalcium phosphate (PLGA)/β-TCP is both bioactive and biodegradable, and is considered a third generation biomaterial for suture anchors. This study aims to describe the incidence of adverse events and the trajectory of patient-reported outcomes up to 12 months follow up in patients undergoing rotator cuff repair with this type of anchor.MethodsA sub-group analysis of a prospective clinical registry embedded in an orthopaedic clinic was conducted. Patients undergoing surgery with the Healix Advance BR were identified and data on patient demographics, treatment details, complications and adverse events, as well as patient-reported outcomes (QuickDASH, WORC Index Normalised) were retrieved. Summary statistics were generated for patient characteristics and PROMs analysed using multiple imputation and a linear model to assess changes between baseline and 12 month follow up.ResultsA consecutive cohort of 68 cases receiving the anchor of interest was included for analysis. Complications were recorded in six cases (8.8%, 95%CI 3.6 - 18.9), presenting as postoperative capsulitis/stiffness (N=3), persistent shoulder pain (N=1), a case of hemi-diaphragm palsy and a case of complex regional pain syndrome in the hand. No infections or reoperations were encountered in this series. The QuickDASH scores improved significantly from 45 (IQR 34-57) preoperatively to 5 (IQR 2-22) at 12 months. WORC Index scores improved from 46 (IQR 27-60) preoperatively to 85 (IQR 62-93) at 12 months.ConclusionThis study found a low incidence of adverse events, no requirement for reoperation or revision, and favourable recovery of patient-reported outcomes in patients treated with arthroscopic rotator cuff repair with a third-generation biocomposite suture anchor at up to 12 months follow up.
PI(3,5)P2asymmetry during mitosis is essential for asymmetric vacuolar inheritance
Authors: Huda Mariam , Koyuncu Mukadder , Dilege Cansu , Caydasi Ayse Koca 
Published: June 2024

AbstractPhosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling lipid that plays crucial roles in various cellular processes, including endolysosomal system structure/function, stress response, and cell cycle regulation. PI(3,5)P2synthesis increases in response to environmental stimuli, yet its behavior in cycling cells under basal conditions remained elusive. Here, we analyzed spatiotemporal changes in PI(3,5)P2levels during the cell cycle ofS. cerevisiae.We found that PI(3,5)P2accumulates on the vacuole in the daughter-cell while it disappears from the vacuole in the mother-cell during mitosis. Concomitant with the changes in PI(3,5)P2distribution, the daughter-vacuole became more acidic, whereas the acidity of the mother-vacuole decreased during mitosis. Our data further showed that both PI(3,5)P2and the PI(3,5)P2effector protein Atg18 are determinants of vacuolar-pH asymmetry and acidity. Our work, thus, identifies PI(3,5)P2as a key factor for establishment of vacuolar-pH asymmetry, providing insights into how the mother cell ages while the daughter cell is rejuvenated.
Redistribution of fragmented mitochondria ensure symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes
Authors: Gekko Haruna , Nomura Ruri , Kuzuhara Daiki , Kaneyasu Masato , Koseki Genpei , Adhikari Deepak , Mio Yasuyuki , Carroll John , Kono Tomohiro , Funahashi Hiroaki , Wakai Takuya 
Published: June 2024

AbstractIn cleavage-stage embryos, preexisting organelles partition evenly into daughter blastomeres without significant cell growth after symmetric cell division. The presence of mitochondrial DNA within mitochondria and its restricted replication during preimplantation development makes their inheritance particularly important. While chromosomes are precisely segregated by the mitotic spindle, the mechanisms controlling mitochondrial partitioning remain poorly understood. In this study, we investigate the mechanism by which Dynamin-related protein 1 (Drp1) controls the mitochondrial redistribution and partitioning during embryonic cleavage. Deletion of Drp1 in mouse zygotes causes marked mitochondrial aggregation, and the majority of embryos arrest at the 2-cell stage. Clumped mitochondria are located in the center of mitotic Drp1-depleted zygotes with less uniform distribution, thereby preventing their symmetric partitioning. Asymmetric mitochondrial inheritance is accompanied by functionally inequivalent blastomeres with biased ATP and endoplasmic reticulum Ca2+levels. We also find that marked mitochondrial centration in Drp1-depleted zygotes prevents the assembly of parental chromosomes, resulting in chromosome segregation defects and binucleation. Thus, mitochondrial fragmentation mediated by Drp1 ensure proper organelle positioning and partitioning into functional daughters during the first embryonic cleavage.Impact statementDepletion of Dynamin-related protein 1, a key regulator of mitochondrial fission, in mouse zygotes impair symmetric organelle partitioning and chromosome segregation leading to early developmental arrest.
Graduate employability skills, trending avenues and research gaps: a systematic literature review and bibliometric analysis
Authors: Amarathunga Buddhini , Khatibi Ali , Talib Zunirah Mohd , Azam S.M. Ferdous , Tham Jacquline 
Published: June 2024

PurposeCurrent study theoretically and technically analyzed the empirical literature on Graduate Employability Skills (GES) and aimed to investigate ten research questions: 1) the specific features of the retrieved empirical studies on GES, 2) the trends of empirical scientific production of GES, 3) the most relevant and high-impact sources in the field of GES, 4) clustering the sources through Bradford’s Law of Scattering, 5) the highly cited articles on GES, 6) the most relevant countries on GES, 7) the most pertinent and high-impact authors on GES, 8) authors' productivity through Lotka’s Law of authors’ Scientific Productivity, 9) the trending research avenues for future investigations on GES, and 10) identified research gaps relevant to the field of GES.Design/methodology/approachThe Scopus database was used to extract data, and VOSviewer and Biblioshony tools were used for the study's bibliometric analysis and systematic literature evaluation.FindingsThe present study analyzed 864 sources containing 1816 articles from 4378 authors that address GES. Publications on GES were steadily increasing, with a notable upswing beginning in 2010 and reaching a record high of 232 articles in 2019. The UK, Australia, and Malaysia are the top three nations in terms of number of publications and cumulative citations. The thematic map of keywords revealed which themes future researchers need to investigate: work-integrated learning, entrepreneurship, industry 4.0, sustainability, management education, business education, project-based learning, education, curriculum development, learning, and graduate skills.Originality/valueThe present study provides theoretical, practical, and social implications for graduates, the higher education industry, policymakers, the economy, and society.
Greening the future: analyzing green entrepreneurial orientation, green knowledge management and digital transformation for sustainable innovation and circular economy
Authors: Al Halbusi Hussam , Popa Simona , Alshibani Safiya Mukhtar , Soto-Acosta Pedro 
Published: June 2024

PurposeGreen innovation, digitization and sustainability have attracted considerable attention in recent years due to their transformative potential in organizations. This study, grounded in the resource-based view, explores the intricate relationship among green entrepreneurial orientations, sustainability-oriented innovation, and the circular economy, employing green knowledge management as a pivotal mechanism.Design/methodology/approachThe proposed model and hypotheses were tested using Partial Least Squares (PLS) structural equation modeling (SEM) with a sample of 274 valid questionnaires collected from manufacturing firms in Saudi Arabia.FindingsResults unveil positive relationships between green entrepreneurial orientations and sustainability-oriented innovation, as well as the circular economy, along with a positive mediation of green knowledge management in these relationships. In addition, these relationships exhibit heightened strength with increased levels of digital transformation.Originality/valueThe contributions of this research extend to both theoretical and practical realms, offering valuable insights for startups and traditional businesses as they explore the landscape of green innovation and digitization.
Good for all, good for me: the influences of dedication- and constraint-based switching barriers on user-generated contributions
Authors: Chou En-Yi , Lin Cheng-Yu 
Published: June 2024

PurposePrior research on user-generated content (UGC) contributions has primarily focused on self-centered or other-centered motives, paying limited attention to the concept of enlightened self-interest, in which both motives coexist in a single organism. Additionally, the factors influencing enlightened self-interest and their effects in different circumstances are yet to be explored. Drawing on theoretical lenses rooted in the switching barriers perspective and stimulus–organism–response framework, this study posits that dedication-based switching barriers (community–member relationship quality, member–member relationship quality, and content attractiveness) positively relate to enlightened self-interest, whereas constraint-based switching barriers (switching costs) moderate the relationship between dedication-based switching barriers and enlightened self-interest in social media communities (SMCs). Members' enlightened self-interest in turn influences both the creation and co-creation of UGC.Design/methodology/approachThis study comprised two quantitative studies: an online survey-based study (Study 1) and an online scenario-based experiment (Study 2). Study 1 surveyed 613 respondents, while Study 2 included 749 participants. Both studies employed structural equation modeling and bootstrapping techniques for analysis.FindingsThe findings indicate that dedication-based switching barriers positively affect users' enlightened self-interest, which in turn is positively associated with UGC creation and co-creation. Switching costs moderate the relationship between relationship quality (community–member and member–member) and enlightened self-interest.Originality/valueThis study complements the current understanding of how the association between dedication- and constraint-based switching barriers and users' enlightened self-interests influence user-generated contributions.
Safety and feasibility of minimally invasive distal pancreatectomy for pancreatic cancer in elderly patients: A retrospective study
Authors: Aoyama Shu , Ohmura Yoshiaki , Takeda Yutaka , Katsura Yoshiteru , Kinoshita Mitsuru , Shinke Go , Kihara Yukari , Yanagisawa Kiminori , Katsuyama Shinsuke , Ikeshima Ryo , Hiraki Masayuki , Sugimura Keijiro , Masuzawa Toru , Hata Taishi , Murata Kohei 
Published: June 2024

AbstractIntroductionPrevious studies have not evaluated the surgical difficulty of minimally invasive distal pancreatectomy for pancreatic cancer in elderly patients. Therefore, we aimed to investigate the effect of elderly age on the perioperative outcomes of minimally invasive distal pancreatectomy, focusing on surgical difficulty.MethodsThis single‐center retrospective study included patients who underwent minimally invasive distal pancreatectomy for pancreatic cancer at Kansai Rosai Hospital between September 2012 and December 2023. Perioperative outcomes were investigated between the elderly (>75 years) and non‐elderly (≤75 years) groups.ResultsFifty‐six patients were included: 26 and 30 in the elderly and non‐elderly groups, respectively. The median operative time was significantly shorter in the elderly group than in the non‐elderly group (324 vs. 414 min, p = .022), but other surgical outcomes were not significantly different including oncological factors. The median difficulty score was similar between the elderly and non‐elderly groups (6 vs. 7, respectively; p = .699). The incidences of postoperative complications and pancreatic fistulas were not significantly different in the elderly and non‐elderly groups (23% vs. 43%, p = .159, and 19% vs. 36%, p = .236, respectively), even though analyzed in subgroups with low‐to‐intermediate or high difficulty score.ConclusionsThe safety and feasibility of minimally invasive distal pancreatectomy for pancreatic cancer were not significantly different between elderly and non‐elderly patients, even when surgical difficulty was considered. This surgical procedure can be safe and feasible for elderly patients.
Enmity then empathy: How militarisation facilitated collaborative but exclusive exchange in Sierra Leone's Ebola response
Authors: Boland Samuel T. , Mayhew Susannah H. , Rohan Hana , Lillywhite Louis , Balabanova Dina 
Published: June 2024

AbstractIn the autumn of 2014, with the 2013–16 West Africa Ebola epidemic spiralling out of control, the United Kingdom announced a bespoke military mission to support—and in some ways lead—numerous Ebola response functions in Sierra Leone. This study examines the nature and effect of the civil‐military relationships that subsequently developed between civilian and military Ebola response workers (ERWs). In total, 110 interviews were conducted with key involved actors, and the findings were analysed by drawing on the neo‐Durkheimian theory of organisations. This paper finds that stereotypical opposition between humanitarian and military actors helps to explain how and why there was initial cooperative and collaborative challenges. However, all actors were found to have similar hierarchical structures and operations, which explains how and why they were later able to cooperate and collaborate effectively. It also explains how and why civilian ERWs might have served to exclude and further marginalise some local actors.
Conceptualizing Socialization, Qualification, and Subjectification as Purposes of Education†
Authors: Juvonen Sara , Huilla Heidi , Kosunen Sonja , Thrupp Martin , Toom Auli 
Published: June 2024

AbstractThe authors of this paper explore Gert Biesta's theorization of three domains of purpose of education: socialization, qualification, and subjectification. The aim is to study the interrelations of the domains and to develop further the theoretical discussion concerning schools' purpose for both individuals and society. Outlining the relationships of the domains of purpose allows one to see how the societal purpose of education is realized in the education of individual students. The domain of socialization sets the stage for the domains of qualification and subjectification, making the balance of the latter two crucial for disadvantaged students as well as schools' societal purpose of both maintaining and reforming soci(et)al structures. Two key ideas are raised: First, in order to better understand the current dynamics of the three domains, the authors suggest separating the logic and content of “qualification.” Second, the authors discuss why it is equally useful to separate the everyday and societal dimension of purpose. The central question is this: Can individual educators find a balance between the three domains if the surrounding society overvalues the logic of qualification?
Nutritional enrichment of cereal foods: feasibility study and characterisation of biscuits fortified with seabass by‐products
Authors: Honrado Adrián , Ardila Paula , Leciñena Paula , Beltrán José A. , Calanche Juan 
Published: June 2024

SummaryIndustrial fish processing generates large amounts of fish by‐products. Therefore, this research aimed to explore alternatives that allow better exploitation of these by‐products and promote sustainability and a circular economy. To achieve this, the possibility of producing and using fish meal (FM) and fish protein hydrolysates (FPH) from seabass was studied for the nutritional fortification of biscuits. The results showed that the heat applied in the production of FPH was a factor that negatively affected the lipidic fraction, reducing the proportion of polyunsaturated fatty acids but also producing a product with darker shades. The incorporation of FM and FPH into the biscuits produced nutritional enrichment, especially in protein and polyunsaturated fatty acids, but also caused colour changes, which were more intense due to the increase of Maillard reaction, and changes in sensory perception, where higher intensities of colour and toasted flavour were perceived (especially when including FPH), but also fish flavours. Scanning electron microscopy made possible the visualisation of differences in the internal structure, which could be related to differences found in instrumental texture measurements. In conclusion, this research demonstrated that using fish by‐products to enrich biscuits is possible from a nutritional perspective. However, other techniques such as the use of antioxidant substances to preserve the fish's fatty acid profile or the combination of enzymes to reduce fish flavours should be considered for future research.
Prevalence of physical inactivity and its determinants among older adults living in nursing homes: A cross‐sectional study based on COM‐B model
Authors: Shi Ying , Xie Xi‐yan , Lao Ai‐di , Shao Lu , Wang Zhang‐an , Zhang Jun‐e 
Published: June 2024

AbstractAimsTo investigate the prevalence of physical inactivity in older adults living in nursing homes and explore the determinants of physical inactivity by using the Capability, Opportunity, Motivation–Behaviour model.DesignA multisite, cross‐sectional study was performed by convenience sampling and questionnaire survey.MethodsA total of 390 nursing home residents were recruited from three nursing homes in Southern China from May 2022 to April 2023. The participants completed a self‐designed general information questionnaire, Physical Activity Scale for the Elderly, Self‐Efficacy for Exercise Scale, Exercise Benefits Scale, Patient Health Questionnaire‐9 and the Short Physical Performance Battery test. Descriptive statistics, univariate analysis, Spearman correlation analysis, and ordinal logistic regression were applied for data analysis.ResultsThe prevalence of physical inactivity among the nursing home residents reached 88.46%. Ordinal logistic regression results showed that exercise self‐efficacy, perceived exercise benefits, physical function, availability of physical activity instruction, having depression, number of chronic diseases and living with spouse were the main influencing determinants of physical inactivity and explained 63.7% of the variance.ConclusionsPhysical inactivity was considerable in nursing home residents in China and influenced by complex factors. Tailored measures should be designed and implemented based on these factors to enhance physical activity while considering the uniqueness of Chinese culture.Implications for the profession and patient careHealthcare professionals should enhance physical activity of residents by increasing benefits understanding, boosting self‐efficacy, improving physical function, alleviating depression and integrating personalized physical activity guidance into routine care services. And more attention should be paid to the residents who had more chronic diseases or did not live with spouse.ImpactPhysical inactivity is a significant problem in nursing home residents. Understanding physical inactivity and its determinants enables the development of tailored interventions to enhance their physical activity level.Reporting methodThis study was reported conforming to the STROBE statement.Patients or Public ContributionNursing home residents who met the inclusion criteria were recruited.