bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrospun polylactide/poly(ethylene glycol) hybrid fibrous scaffolds for tissue engineering
Authors: Wang Bei‐Yu , Fu Shao‐Zhi , Ni Pei‐Yan , Peng Jing‐Rong , Zheng Lan , Luo Feng , Liu Hao , Qian Zhi‐Yong 
Published: November 2011

AbstractThe biodegradable polylactide/poly(ethylene glycol) (PLA/PEG) hybrid membranes were fabricated via electrospinning of PLA/PEG solution. Their structures and properties were investigated by scanning electron microscopy, differential scanning calorimetry, and water contact angle. In vitro hydrolytic degradation showed that PEG content influenced the degradation rate of the PLA/PEG hybrid mats. The mechanical property was measured by tensile test and the result revealed that the addition of PEG had an obvious plasticization on PLA matrix. In‐vitro biocompatibility was investigated by culturing cell on the scaffolds and MTT assay. The results indicated that the cell could attach and proliferate on the membranes, so confirmed that the PLA/PEG hybrid membrane had good biocompatibility, and it could be a promising biomaterial for tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Effects of the iron‐chelating agent deferoxamine on triethylene glycol dimethacrylate, 2‐hydroxylethyl methacrylate, hydrogen peroxide‐induced cytotoxicity
Authors: Zhu Tingting , Lim Bum‐Soon , Park Hee Chul , Son Kyung Mi , Yang Hyeong‐Cheol 
Published: November 2011

AbstractTriethylene glycol dimethacrylate (TEGDMA) and 2‐hydroxylethyl methacrylate (HEMA) are known to deplete glutathione in mammalian cells, generate reactive oxygen species (ROS), and cause oxidative stress. In this study, we investigated whether hydroxyl radicals (·OH), the most lethal and genotoxic ROS, and the Fenton reaction are involved in the cytotoxicity of resin monomers to four different cell types, namely MC3T3‐E1 preosteoblasts, human dental pulp cells (HDPCs), human gingival fibroblasts, and L929 mouse fibroblasts. Deferoxamine (DFO), an iron chelating agent, effectively protected MC3T3‐E1 cells from resin monomer‐induced cytotoxicity, indicating that cytotoxicity was caused primarily by hydroxyl radicals. However, DFO only had a protective effect against relatively high concentrations of TEGDMA and HEMA in HDPCs and human gingival fibroblasts, and resin monomer‐induced cytotoxicity in L929 was not attenuated by DFO. A labile iron pool (LIP) was detectable only in MC3T3‐E1 cells among the four cell types. This indicates that the generation of hydroxyl radicals induced by resin monomers is likely dependent on LIP levels. In contrast to resin monomers, hydrogen peroxide (H2O2)‐induced cytotoxicity was not prevented by DFO in any of the cell types examined, although hydroxyl radicals were detected in MC3T3‐E1 cells and HDPCs on exposure to exogenous H2O2. This result suggests that generation of hydroxyl radicals is not always the primary cause of cytotoxicity in H2O2‐treated cells. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Clearance kinetics of a hylan‐based viscosupplement after intra‐articular and intravenous administration in animal models
Authors: Larsen Nancy E. , Dursema Holly D. , Pollak Cynthia T. , Skrabut Eugene M. 
Published: November 2011

AbstractClearance of hylan fluid and hylan gel, components of the hyaluronan (HA)‐derived viscosupplement hylan G‐F 20, following intra‐articular injection into normal, healthy rabbits was evaluated. Radiolabeled hylan G‐F 20 was injected at a volume of 0.3 mL into both knee joints of 12 rabbits. At sacrifice, synovial fluid, joint tissues, blood, popliteal lymph nodes, liver, spleen, kidney, and lung were analyzed for radioactivity. The half‐life of the fluid component, a high‐molecular weight hylan, was 1.5 ± 0.2 days while the half‐life of the hylan gel component, a crosslinked hylan, was 8.8 ± 0.9 days. There was no radioactivity detected in the blood or the major internal organs following intra‐articular injection. A rat model was used to evaluate the clearance of a large intravenous bolus of solubilized hylan gel. No accumulation of hylan gel degradation products was observed in any major organs and the half‐life of hylan elimination from the blood was within normal ranges for HA elimination. The dosing used in the nonclinical rabbit intra‐articular study was equivalent (v/w) to a single 6 mL dose in humans. These results are consistent with the current clinical data that demonstrates safety and effectiveness of an increased volume of hylan G‐F 20 injected into the osteoarthritis knee. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 457–462, 2012.
Is degradable antibiotic coating for synthetic meshes provide protection against experimental animal infection after fascia repair?
Authors: Letouzey Vincent , Lavigne Jean Philippe , Garric Xavier , Coudane Jean , de Tayrac Renaud , Callaghan David O. 
Published: November 2011

AbstractThe surgical repair of pelvic organ prolapse using synthetic mesh can fail because of slow or partial implant integration due to poor biocompatibility or infection. As systemic antibiotic prophylaxis has only limited success, we have developed a system that coats standard polypropylene mesh with clinically relevant antibiotics. Amoxicillin and ofloxacin are both released from the mesh in vitro at high levels over 3 days, preventing adhesion and biofilm formation by a clinical isolate of E. coli. In an in vivo incisional hernia repair model in rats, the antibiotic‐coated mesh results in appropriate tissue integration with adequate vascularization and collagen formation. When implanted animals are infected with virulent E. coli, both antibiotic coatings provide full protection against infection (as assessed both clinically and microbiologically), thus demonstrating their bioavailability. This method is a specific approach for producing a therapeutic coating that could reduce postsurgical infections. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 471–479, 2012.
Macrophage reactivity to different polymers demonstrates particle size‐ and material‐specific reactivity: PEEK‐OPTIMA®particles versus UHMWPE particles in the submicron, micron, and 10 micron size ranges
Authors: Hallab Nadim James , McAllister Kyron , Brady Mark , Jarman‐Smith Marcus 
Published: November 2011

AbstractBiologic reactivity to orthopedic implant debris is generally the main determinant of long‐term clinical performance where released polymeric particles of Ultra‐high molecular weight polyethylene (UHMWPE) remain the most prevalent debris generated from metal‐on‐polymer bearing total joint arthroplasties. Polymeric alternatives to UHMWPE such as polyetherether‐ketone (PEEK) may have increased wear resistance but the bioreactivity of PEEK‐OPTIMA particles on peri‐implant inflammation remains largely uncharacterized. We evaluated human monocyte/macrophage responses (THP‐1s and primary human) when challenged by PEEK‐OPTIMA, UHMWPE, and X‐UHMWPE particles of three particle sizes (0.7 um, 2 um, and 10 um) at a dose of 20 particles‐per‐cell at 24‐ and 48‐h time points. Macrophage responses were measured using cytotoxicity assays, viability assays, proliferation assays and cytokine analysis (IL‐1b, IL‐6, IL‐8, MCP‐1, and TNF‐α). In general, there were no significant differences between PEEK‐OPTIMA, UHMWPE, and X‐UHMWPE particles on macrophage viability or proliferation. However, macrophages demonstrated greater cytotoxicity responses to UHMWPE and X‐UHMWPE than to PEEK‐OPTIMA at 24 and 48 h, where 0.7 μm‐UHMWPE particles produced the highest amount of cytotoxicity. Particles of X‐UHMWPE more than PEEK‐OPTIMA and UHMWPE induced IL‐1β, IL‐6, MCP‐1, and TNF‐α at 24 h,p< 0.05 (no significant differences at 48 h). On average, cytokine production was more adversely affected by larger 10 μm particles than by 0.7 and 2 μm sized particles. While limitations ofin vitroanalysis apply to this study, PEEK‐OPTIMA particles were more biocompatible than UHMWPE particles, in that they induced less inflammatory cytokine responses and thus, in part, demonstrates that PEEK‐OPTIMA implant debris does not represent an increased inflammatory risk over that of UHMWPE. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 480–492, 2012.
Molecular chaperones in mammary cancer growth and breast tumor therapy
Authors: Calderwood Stuart K. , Gong Jianlin 
Published: November 2011

AbstractHeat shock protein (HSP) levels are elevated in breast cancer and are molecular targets for novel therapies. HSPs were first observed as proteins induced in massive amounts in normal cells exposed to stresses that lead to protein denaturation. Their expanded expression in mammary carcinoma appears to be largely due to the proliferation of malfolded mutant proteins and overexpressed oncoproteins that trigger transcription of HSP genes. HSPs play major roles in malignant transformation and progression mediated through their intrinsic molecular chaperone properties. These permit the emergence of new malignant traits through the facilitated accumulation of altered oncoproteins. The elevation of HSP concentrations in mammary carcinoma is at least partially dependent on heat shock transcription factor 1 (HSF1), a protein that responds to unfolded proteins and leads to HSP transcription. HSF1 activation has additional downstream activities, crucial for emergence of the breast cancer phenotype and these include activated cell signaling, HSP‐mediated ability to evade apoptosis and senescence and an HSF1‐dependent bias in transcriptional activity towards a metastatic phenotype. The HSPs are currently being targeted in breast cancer therapy and effective drugs for Hsp90 have been synthesized and evaluated in clinical trial. Mammary carcinoma cells also contain abundant quantities of HSP–tumor antigen complexes and these complexes are being used to develop effective tumor vaccine approaches that provide personalized therapy for each individual's cancer. J. Cell. Biochem. 113: 1096–1103, 2012. © 2011 Wiley Periodicals, Inc.
Treatment satisfaction, perceived treatment effectiveness, and dropout among older users of mental health services
Authors: Lippens Tiffany , Mackenzie Corey S. 
Published: November 2011

AbstractObjectives: To examine the rates and correlates of treatment satisfaction, perceived treatment effectiveness, and dropout among older users of mental health services. Method: We used data from the Canadian Community Health Survey‐Mental Health and Well‐Being (CCHS‐1.2), which includes 12,792 individuals aged ≥55 years. The average age of these participants was 67 years and 53.2% were female. We examined the rates of treatment satisfaction, perceived treatment effectiveness, and dropout for those who had used mental health services in the past year, and used logistic regression to examine the correlates of these outcomes. Results: Of the older adults included in the CCHS‐1.2, 664 (5.3%) had used mental health services in the past year. The majority of these were satisfied with services (88.5%) and perceived treatment to be effective (83.6%), which is likely why only 15.5% dropped out in the past year. In logistic regression models, social support was significantly and positively related to both treatment satisfaction and perceived effectiveness. Perceived treatment effectiveness was the only variable related to dropout, with lower levels of perceived effectiveness associated with greater odds of dropping out of treatment. Conclusions: Results from this study indicate that older adults have very good self‐reported treatment outcomes. The modest influence of individual characteristics on treatment outcomes suggests the potential importance of contextual characteristics. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1197–1209, 2011.
Crosstalk between adenosine A1 and β1‐adrenergic receptors regulates translocation of PKCε in isolated rat cardiomyocytes
Authors: Komatsu Satoshi , Dobson James G. , Ikebe Mitsuo , Shea Lynne G. , Fenton Richard A. 
Published: November 2011

AbstractAdenosine A1 receptor (A1R)‐induced translocation of PKCε to transverse (t) tubular membranes in isolated rat cardiomyocytes is associated with a reduction in β1‐adrenergic‐stimulated contractile function. The PKCε‐mediated activation of protein kinase D (PKD) by endothelin‐1 is inhibited by β1‐adrenergic stimulated protein kinase A (PKA) suggesting a similar mechanism of A1R signal transduction modulation by adrenergic agonists may exist in the heart. We have investigated the influence of β1‐adrenergic stimulation on PKCε translocation elicited by A1R. Immunofluorescence imaging and Western blotting with PKCε and β‐COP antibodies were used to quantify the co‐localization of PKCε and t‐tubular structures in isolated rat cardiomyocytes. The A1R agonist CCPA increased the co‐localization of PKCε and t‐tubules as detected by imaging. The β1‐adrenergic receptor agonist isoproterenol (ISO) inhibited this effect of CCPA. Forskolin, a potent activator of PKA, mimicked, and H89, a pharmacological PKA inhibitor, and PKI, a membrane‐permeable PKA peptide PKA inhibitor, attenuated the negative effect of ISO on the A1R‐mediated PKCε translocation. Western blotting with isolated intact hearts revealed an increase in PKCε/β‐COP co‐localization induced by A1R. This increase was attenuated by the A1R antagonist DPCPX and ISO. The ISO‐induced attenuation was reversed by H89. It is concluded that adrenergic stimulation inhibits A1R‐induced PKCε translocation to the PKCε anchor site RACK2 constituent of a coatomer containing β‐COP and associated with the t‐tubular structures of the heart. In that this translocation has been previously associated with the antiadrenergic property of A1R, it is apparent that the interactive effects of adenosine and β1‐adrenergic agonists on function are complex in the heart. J. Cell. Physiol. 227: 3201–3207, 2012. © 2011 Wiley Periodicals, Inc.
Differential mesengenic potential and expression of stem cell‐fate modulators in mesenchymal stromal cells from human‐term placenta and bone marrow
Authors: Jaramillo‐Ferrada Pamela A. , Wolvetang Ernst J. , Cooper‐White Justin J. 
Published: November 2011

AbstractPlacenta has attracted increasing attention over the past decade as a stem cell source for regenerative medicine. In particular, the amniochorionic membrane has been shown to harbor populations of mesenchymal stromal cells (MSCs). In this study, we have characterized ex vivo expanded MSCs from the human amniotic (hAMSCs) and chorionic (hCMSCs) membranes of human full‐term placentas and adult bone marrow (hBMSCs). Our results show that hAMSCs, hCMSCs, and hBMSCs express typical mesenchymal (CD73, CD90, CD105, CD44, CD146, CD166) and pluripotent (Oct‐4, Sox2, Nanog, Lin28, and Klf4) markers but not hematopoietic markers (CD45, CD34). Ex vivo expanded hAMSCs were found to be of fetal origin, while hCMSCs cultures contained only maternal cells. Cell proliferation was significantly higher in hCMSCs, compared to hAMSCs and hBMSCs. Integrin profiling revealed marked differences in the expression of α subunits between the three cell sources. Cadherin receptors were consistently expressed on a subset of progenitors (ranging from 1% to 60%), while N‐CAM (CD56) was only expressed in hAMSCs and hCMSCs but not in hBMSCs. When induced to differentiate, hAMSCs and hCMSCs displayed strong chondrogenic and osteogenic differentiation potential but very limited capacity for adipogenic conversion. In contrast, hBMSCs showed strong differentiation potential along the three lineages. These results illustrate how MSCs from different ontological sources display differential expression of cell‐fate mediators and mesodermal differentiation capacity. J. Cell. Physiol. 227: 3234–3242, 2012. © 2011 Wiley Periodicals, Inc.
New insights in endometrial carcinogenesis
Authors: D'Andrilli Giuseppina , Bovicelli Alessandro , Paggi Marco G. , Giordano Antonio 
Published: November 2011

AbstractEndometrial carcinoma is the most common cancer of the female genital tract in Europe and in the United States. Despite advances in defining the biology of endometrial carcinomas, there has been poor progress in determining markers that distinguish preinvasive endometrial proliferations. The aim of this review is to highlight the most recent studies regarding the molecular markers involved in endometrial adenocarcinoma pathogenesis and carcinogenesis. We focus on studies that describe markers with potential to progress from endometrial hyperplasia to invasive disease. J. Cell. Physiol. 227: 2842–2846, 2012. © 2011 Wiley Periodicals, Inc.
Bone production by human maxillary sinus mucosa cells
Authors: Graziano A. , Benedetti L. , Massei G. , Cusella de Angelis M.G. , Ferrarotti F. , Aimetti M. 
Published: November 2011

AbstractThe Schneider membrane is the mucosa that covers the inner part of the maxillary sinus cavities. The free surface is a ciliated pseudostratified epithelium, while the deeper portion is a highly vascularized connective tissue. The stromal fraction, bordering the bony wall of the sinus, after tooth loss can exhibit increased osteoclastic activity resulting in resorption of the bone in the posterior maxilla. Goal of our study was to isolate and characterize mesenchymal progenitors in the Schneider's membrane connective net and to evaluate their self ability to differentiate toward osteoblastic lineage, in absence of osteoinductive factors and osteoconductive biomaterials of support. This should indicate that maxillary sinus membrane represents an useful an approachable source of MSCs for bone tissue engineering and cell therapy and owns the intrinsic capacity to restore maxillary bone after tooth loss without the needing of biomaterials. J. Cell. Physiol. 227: 3278–3281, 2012. © 2011 Wiley Periodicals, Inc.
Sugarcane bagasse and leaves: foreseeable biomass of biofuel and bio‐products
Authors: Chandel Anuj K. , da Silva Silvio S. , Carvalho Walter , Singh Om V. 
Published: November 2011

AbstractSugarcane is among the principal agricultural crops cultivated in tropical countries. The annual world production of sugarcane is ∼1.6 billion tons, and it generates ∼279 million metric tons (MMT) of biomass residues (bagasse and leaves). Sugarcane residues, particularly sugarcane bagasse (SB) and leaves (SL) have been explored for both biotechnological and non‐biotechnological applications. For the last three decades, SB and SL have been explored for use in lignocellulosic bioconversion, which offers opportunities for the economic utilization of residual substrates in the production of bioethanol and value‐added commercial products such as xylitol, specialty enzymes, organic acids, single‐cell protein, etc. However, there are still major technological and economic challenges to be addressed in the development of bio‐based commercial processes utilizing SB and SL as raw substrates. This article aims to explore SB and SL as cheaper sources of carbohydrates in the developing world for their industrial implications, their use in commercial products including commercial evaluation, and their potential to advance sustainable bio‐based fuel systems. Copyright © 2011 Society of Chemical Industry
Early prenatal sonographic diagnosis of gastroschisis
Authors: Cerekja Albana , Piazze Juan , Cozzi Denis 
Published: November 2011

AbstractWe report a case of gastroschisis that was suspected at sonography at 11 weeks + 3 days of amenorrhea. Although early detection does not alter prenatal management of this condition nor does it affect neonatal outcome, it allows early counseling ofthe parents.©2011 Wiley Periodicals, Inc. J Clin Ultrasound 40:526–528, 2012
Mechanical properties of the avian acrocoracohumeral ligament and its role in shoulder stabilization in flight
Authors: Baier David Bradley 
Published: November 2011

AbstractControl of movement in the avian shoulder joint is fundamental to understanding the avian wingstroke. The acrocoracohumeral ligament (AHL) is thought to play a key role in stabilizing the glenoid and balancing the pectoralis in gliding flight. If the AHL has to be taut to balance the pectoralis, then it must constrain glenohumeral motion during flapping flight as well. However, birds vary wing kinematics depending on flight speed and behavior. How can a passive ligament accommodate such varying joint movements? Herein, mechanical testing and 3‐D modeling are used to link the mechanical properties and morphology of the AHL to its functional role during flapping flight. The bone–ligament–bone complex of the pigeon (Columba livia) fails at a tensile loading of 141±18 N (±s.d., n = 10) or 39 times body weight, which corresponds to a failure stress of 51 MPa, well above expected loads during flight. Simulated AHL length changes, comparisons to glenohumeral kinematics from the literature, and manipulations of partially dissected pigeon specimens all support the hypothesis that the AHL remains taut through downstroke and most of upstroke while becoming slack during the downstroke/upstroke transition. The digital AHL model provides a mechanism for explaining how the AHL can stabilize the shoulder joint under a broad array of humeral paths by constraining the coordination of glenohumeral degrees of freedom. J. Exp. Zool. 317:83–95, 2012. © 2011 Wiley Periodicals, Inc.
Tetrakis(acetonitrile)copper(I) hexafluorophosphate catalyzed coumarin synthesis via pechmann condensation under solvent‐free condition
Authors: Soleimani Ebrahim , Khodaei Mohammad Mehdi , Batooie Nasim , Samadi Saadi 
Published: November 2011

AbstractTetrakis(acetonitrile)copper(I) hexafluorophosphate (Cu(CH3CN)4PF6) is used as an efficient catalyst in the Pechmann condensation reaction of phenols with ethyl acetoacetate leading to the formation of coumarin derivatives in excellent yields under solvent free conditions at ambient temperature. The method is simple, solvent‐free and gives excellent yields in a short reaction time. J. Heterocyclic Chem.,(2011).
A multilevel model of emotional skills, communication performance, and task performance in teams
Authors: Troth Ashlea C. , Jordan Peter J. , Lawrence Sandra A. , Tse Herman H. M. 
Published: November 2011

SummaryThere is increasing research regarding the influence of emotions on teamwork. In this study, we use a multilevel approach to examine how team members' use of emotion‐related skills affects team task performance and communication performance within the team. We measured individual self‐reported emotional skills prior to team formation and then collected peer‐rated individual communication performance and independently rated team task performance eight weeks later. Although there was no influence at the individual level between emotional skills and performance, team‐level emotional skills positively predicted team task performance scores. At the cross level, team‐level emotional skills predicted individual‐level communication performance. These findings emphasize the importance of distinct team emotional skills in shaping both team performance and individual team member performance. Copyright © 2011 John Wiley & Sons, Ltd.