bluuebunny/crossref_metadata_2025_split huggingface dataset:
The maternal drug exposure birth cohort (DEBC) in China
Authors: Li Lu , Wang Ke , Wang Meixian , Tao Jing , Li Xiaohong , Liu Zhen , Li Nana , Qiu Xiaoxia , Wei Hongwei , Lin Yuan , He Yuan , Deng Ying , Kang Hong , Li Yuting , Yu Ping , Wang Yanping , Zhu Jun , Liu Hanmin 
Published: June 2024

AbstractDrug exposure during pregnancy lacks global fetal safety data. The maternal drug exposure birth cohort (DEBC) study, a prospective longitudinal investigation, aims to explore the correlation of maternal drug exposure during pregnancy with pregnancy outcomes, and establish a human biospecimen biobank. Here we describe the process of establishing DEBC and show that the drug exposure rate in the first trimester of pregnant women in DEBC (n = 112,986) is 30.70%. Among the drugs used, dydrogesterone and progesterone have the highest exposure rates, which are 11.97% and 10.82%, respectively. The overall incidence of adverse pregnancy outcomes is 13.49%. Dydrogesterone exposure during the first trimester is correlated with higher incidences of stillbirth, preterm birth, low birth weight, and birth defects, along with a lower incidence of miscarriage/abortion. Due to the limitations of this cohort study, causative conclusions cannot be drawn. Further follow-up and in-depth data analysis are planned for future studies.
Scalable multifunctional MOFs-textiles via diazonium chemistry
Authors: Li Wulong , Yu Zhen , Zhang Yaoxin , Lv Cun , He Xiaoxiang , Wang Shuai , Wang Zhixun , He Bing , Yuan Shixing , Xin Jiwu , Liu Yanting , Zhou Tianzhu , Li Zhanxiong , Tan Swee Ching , Wei Lei 
Published: June 2024

AbstractCellulose fiber-based textiles are ubiquitous in daily life for their processability, biodegradability, and outstanding flexibility. Integrating cellulose textiles with functional coating materials can unlock their potential functionalities to engage diverse applications. Metal-organic frameworks (MOFs) are ideal candidate materials for such integration, thanks to their unique merits, such as large specific surface area, tunable pore size, and species diversity. However, achieving scalable fabrication of MOFs-textiles with high mechanical durability remains challenging. Here, we report a facile and scalable strategy for direct MOF growth on cotton fibers grafted via the diazonium chemistry. The as-prepared ZIF-67-Cotton textile (ZIF-67-CT) exhibits excellent ultraviolet (UV) resistance and organic contamination degradation via the peroxymonosulfate activation. The ZIF-67-CT is also used to encapsulate essential oils such as carvacrol to enable antibacterial activity against E. coli and S. aureus. Additionally, by directly tethering a hydrophobic molecular layer onto the MOF-coated surface, superhydrophobic ZIF-67-CT is achieved with excellent self-cleaning, antifouling, and oil-water separation performances. More importantly, the reported strategy is generic and applicable to other MOFs and cellulose fiber-based materials, and various large-scale multi-functional MOFs-textiles can be successfully manufactured, resulting in vast applications in wastewater purification, fragrance industry, and outdoor gears.
In-situ direct seawater electrolysis using floating platform in ocean with uncontrollable wave motion
Authors: Liu Tao , Zhao Zhiyu , Tang Wenbin , Chen Yi , Lan Cheng , Zhu Liangyu , Jiang Wenchuan , Wu Yifan , Wang Yunpeng , Yang Zezhou , Yang Dongsheng , Wang Qijun , Luo Lunbo , Liu Taisheng , Xie Heping 
Published: June 2024

AbstractDirect hydrogen production from inexhaustible seawater using abundant offshore wind power offers a promising pathway for achieving a sustainable energy industry and fuel economy. Various direct seawater electrolysis methods have been demonstrated to be effective at the laboratory scale. However, larger-scale in situ demonstrations that are completely free of corrosion and side reactions in fluctuating oceans are lacking. Here, fluctuating conditions of the ocean were considered for the first time, and seawater electrolysis in wave motion environment was achieved. We present the successful scaling of a floating seawater electrolysis system that employed wind power in Xinghua Bay and the integration of a 1.2 Nm3 h−1-scale pilot system. Stable electrolysis operation was achieved for over 240 h with an electrolytic energy consumption of 5 kWh Nm−3 H2 and a high purity (>99.9%) of hydrogen under fluctuating ocean conditions (0~0.9 m wave height, 0~15 m s−1 wind speed), which is comparable to that during onshore water electrolysis. The concentration of impurity ions in the electrolyte was low and stable over a long period of time under complex and changing scenarios. We identified the technological challenges and performances of the key system components and examined the future outlook for this emerging technology.
Global patterns of organic carbon transfer and accumulation across the land–ocean continuum constrained by radiocarbon data
Authors: Wang Chenglong , Qiu Yifei , Hao Zhe , Wang Junjie , Zhang Chuchu , Middelburg Jack J. , Wang Yaping , Zou Xinqing 
Published: June 2024

AbstractRadiocarbon (Δ14C) serves as an effective tracer for identifying the origin and cycling of carbon in aquatic ecosystems. Global patterns of organic carbon (OC) Δ14C values in riverine particles and coastal sediments are essential for understanding the contemporary carbon cycle, but are poorly constrained due to under-sampling. This hinders our understanding of OC transfer and accumulation across the land–ocean continuum worldwide. Here, using machine learning approaches and >3,800 observations, we construct a high-spatial resolution global atlas of Δ14C values in river–ocean continuums and show that Δ14C values of river particles and corresponding coastal sediments can be similar or different. Specifically, four characteristic OC transfer and accumulation modes are recognized: the old–young mode for systems with low river and high coastal sediment Δ14C values; the young–old and old–old modes for coastal systems with old OC accumulation receiving riverine particles with high and low Δ14C values, respectively; and the young–young mode with young OC for both riverine and coastal deposited particles. Distinguishing these modes and their spatial patterns is critical to furthering our understanding of the global carbon system. Specifically, among coastal areas with high OC contents worldwide, old–old systems are largely neutral to slightly negative to contemporary atmospheric carbon dioxide (CO2) removal, whereas young–old and old–young systems represent CO2 sources and sinks, respectively. These spatial patterns of OC content and isotope composition constrain the local potential for blue carbon solutions.
Deciphering spatial domains from spatial multi-omics with SpatialGlue
Authors: Long Yahui , Ang Kok Siong , Sethi Raman , Liao Sha , Heng Yang , van Olst Lynn , Ye Shuchen , Zhong Chengwei , Xu Hang , Zhang Di , Kwok Immanuel , Husna Nazihah , Jian Min , Ng Lai Guan , Chen Ao , Gascoigne Nicholas R. J. , Gate David , Fan Rong , Xu Xun , Chen Jinmiao 
Published: June 2024

AbstractAdvances in spatial omics technologies now allow multiple types of data to be acquired from the same tissue slice. To realize the full potential of such data, we need spatially informed methods for data integration. Here, we introduce SpatialGlue, a graph neural network model with a dual-attention mechanism that deciphers spatial domains by intra-omics integration of spatial location and omics measurement followed by cross-omics integration. We demonstrated SpatialGlue on data acquired from different tissue types using different technologies, including spatial epigenome–transcriptome and transcriptome–proteome modalities. Compared to other methods, SpatialGlue captured more anatomical details and more accurately resolved spatial domains such as the cortex layers of the brain. Our method also identified cell types like spleen macrophage subsets located at three different zones that were not available in the original data annotations. SpatialGlue scales well with data size and can be used to integrate three modalities. Our spatial multi-omics analysis tool combines the information from complementary omics modalities to obtain a holistic view of cellular and tissue properties.
Prospects and limitations of cumate-inducible lentivirus as a tool for investigating VEGF-A-mediated pathology in diabetic retinopathy
Authors: Lelyte Inesa , Rao Vidhya R. , Kalesnykas Giedrius , Ragauskas Symantas , Kaja Simon , Ahmed Zubair 
Published: June 2024

AbstractDiabetic retinopathy (DR) is a multifactorial disease displaying vascular-associated pathologies, including vascular leakage and neovascularization, ultimately leading to visual impairment. However, animal models accurately reflecting these pathologies are lacking. Vascular endothelial growth factor A (VEGF-A) is an important factor in the development of micro- and macro-vascular pathology in DR. In this study, we evaluated the feasibility of using a cumate-inducible lentivirus (LV) mediated expression of vegf-a to understand DR pathology in vitro and in vivo. Retinal pigment epithelial cells (ARPE-19) were transduced with cumate-inducible LV expressing vegf-a, with subsequent analysis of vegf-a expression and its impact on cell proliferation, viability, motility, and permeability. Cumate tolerability in adult Wistar rat eyes was assessed as an initial step towards a potential DR animal model development, by administering cumate via intravitreal injections (IVT) and evaluating consequent effects by spectral domain optical coherence tomography (SD-OCT), flash electroretinography (fERG), ophthalmic examination (OE), and immunohistochemistry. Transduction of ARPE-19 cells with cumate-inducible LV resulted in ~ 2.5-fold increase in vegf-a mRNA and ~ threefold increase in VEGF-A protein secretion. Transduced cells displayed enhanced cell proliferation, viability, permeability, and migration in tube-like structures. However, IVT cumate injections led to apparent retinal toxicity, manifesting as retinal layer abnormalities, haemorrhage, vitreous opacities, and significant reductions in a- and b-wave amplitudes, along with increased microglial activation and reactive gliosis. In summary, while cumate-inducible LV-mediated vegf-a expression is valuable for in vitro mechanistic studies in cellular drug discovery, its use is not a feasible approach to model DR in in vivo studies due to cumate-induced retinal toxicity.
Identifying key morphometrics to post-storm beach recovery through explainable AI
Authors: Thilakarathne Salika , Suzuki Takayuki , Mäll Martin , Banno Masayuki 
Published: June 2024

AbstractIn the context of ongoing discussions about climate change, the focus on beach resilience has gained significant attention in contemporary studies. However, a comprehensive understanding of beach resilience, particularly in the short term, remains limited. This study utilizes a dataset of 104 storm events in Hasaki beach, located on the East coast of Japan, to investigate the 10-day beach recovery. The study considers four types of distinct beach profile patterns based on sandbar formations. Employing XGBoost and the SHAP explanation method, the influence of morphometric indicators on beach resilience were explored. Resilient beach profiles are anticipated to exhibit rapid recovery following erosional storm events. The results reveal that morphometrics play a crucial role in determining the short-term, 10-day, recovery of beaches, with specific morphometric features demonstrating pronounced effects based on profile patterns. The study contributes to the current knowledge of post-storm beach recovery and provides insights that could inform discussions on beach resilience.
From eDNA to decisions using a multi-method approach to restoration planning in streams
Authors: Adams A. J. , Kamoroff C. , Daniele N. R. , Grasso R. L. , Halstead B. J. , Kleeman P. M. , Mengelt C. , Powelson K. , Seaborn T. , Goldberg C. S. 
Published: June 2024

AbstractReintroduction efforts are increasingly used to mitigate biodiversity losses, but are frequently challenged by inadequate planning and uncertainty. High quality information about population status and threats can be used to prioritize reintroduction and restoration efforts and can transform ad hoc approaches into opportunities for improving conservation outcomes at a landscape scale. We conducted comprehensive environmental DNA (eDNA) and visual encounter surveys to determine the distribution of native and non-native aquatic species in two high-priority watersheds to address key uncertainties—such as the distribution of threats and the status of existing populations—inherent in restoration planning. We then used these occurrence data to develop a menu of potential conservation actions and a decision framework to benefit an endangered vertebrate (foothill yellow-legged frog, Rana boylii) in dynamic stream systems. Our framework combines the strengths of multiple methods, allowing managers and conservation scientists to incorporate conservation science and site-specific knowledge into the planning process to increase the likelihood of achieving conservation goals.
Serum vitamin D, blood pressure and hypertension risk in the HUNT study using observational and Mendelian randomization approaches
Authors: Jiang Lin , Sun Yi-Qian , Denos Marion , Brumpton Ben Michael , Chen Yue , Malmo Vegard , Sanderson Eleanor , Mai Xiao-Mei 
Published: June 2024

AbstractLimited studies have triangulated the relationship between serum 25-hydroxyvitamin D [25(OH)D] levels and systolic blood pressure (SBP), diastolic blood pressure (DBP) or hypertension risk utilizing both observational and Mendelian randomization (MR) approaches. We employed data from the Norwegian Trøndelag Health Study (HUNT) to conduct cross-sectional (n = 5854) and prospective (n = 3592) analyses, as well as one-sample MR (n = 86,324). We also used largest publicly available data for two-sample MR. Our cross-sectional analyses showed a 25 nmol/L increase in 25(OH)D was associated with a 1.73 mmHg decrease in SBP (95% CI − 2.46 to − 1.01), a 0.91 mmHg decrease in DBP (95% CI − 1.35 to − 0.47) and 19% lower prevalence of hypertension (OR 0.81, 95% CI 0.74 to 0.90) after adjusting for important confounders. However, these associations disappeared in prospective analyses. One-sample and two-sample MR results further suggested no causal relationship between serum vitamin D levels and blood pressure or hypertension risk in the general population.
The potential of a novel enzyme-based surface plasmon resonance biosensor for direct detection of dopamine
Authors: Jabbari Safoura , Dabirmanesh Bahareh , Daneshjou Sara , Khajeh Khosro 
Published: June 2024

AbstractDopamine is one of the significant neurotransmitters and its monitoring in biological fluids is a critical issue in healthcare and modern biomedical technology. Here, we have developed a dopamine biosensor based on surface plasmon resonance (SPR). For this purpose, the carboxymethyl dextran SPR chip was used as a surface to immobilize laccase as a bioaffinity recognition element. Data analysis exhibited that the acidic pH value is the optimal condition for dopamine interaction. Calculated kinetic affinity (KD) (48,545 nM), obtained from a molecular docking study, showed strong association of dopamine with the active site of laccase. The biosensor exhibited a linearity from 0.01 to 189 μg/ml and a lower detection limit of 0.1 ng/ml (signal-to-noise ratio (S/N) = 3) that is significantly higher than the most direct dopamine detecting sensors reported so far. Experiments for specificity in the presence of compounds that can co-exist with dopamine detection such as ascorbic acid, urea and l-dopa showed no significant interference. The current dopamine biosensor with high sensitivity and specificity, represent a novel detection tool that offers a label-free, simple procedure and cost effective monitoring system.
Hippocampal-dependent navigation in head-fixed mice using a floating real-world environment
Authors: Stuart Sarah A. , Palacios-Filardo Jon , Domanski Aleks , Udakis Matt , Duguid Ian , Jones Matt W. , Mellor Jack R. 
Published: June 2024

AbstractHead-fixation of mice enables high-resolution monitoring of neuronal activity coupled with precise control of environmental stimuli. Virtual reality can be used to emulate the visual experience of movement during head fixation, but a low inertia floating real-world environment (mobile homecage, MHC) has the potential to engage more sensory modalities and provide a richer experimental environment for complex behavioral tasks. However, it is not known whether mice react to this adapted environment in a similar manner to real environments, or whether the MHC can be used to implement validated, maze-based behavioral tasks. Here, we show that hippocampal place cell representations are intact in the MHC and that the system allows relatively long (20 min) whole-cell patch clamp recordings from dorsal CA1 pyramidal neurons, revealing sub-threshold membrane potential dynamics. Furthermore, mice learn the location of a liquid reward within an adapted T-maze guided by 2-dimensional spatial navigation cues and relearn the location when spatial contingencies are reversed. Bilateral infusions of scopolamine show that this learning is hippocampus-dependent and requires intact cholinergic signalling. Therefore, we characterize the MHC system as an experimental tool to study sub-threshold membrane potential dynamics that underpin complex navigation behaviors.
Assessment of TP53 and CDKN2A status as predictive markers of malignant transformation of sinonasal inverted papilloma
Authors: Kwon Soohyeon , Kim Jeong-Whun , Kim Eun Sun , Paik Jin Ho , Chung Jin-Haeng , Cho Sung-Woo , Won Tae-Bin , Rhee Chae-Seo , Wee Jee Hye , Kim Hyojin 
Published: June 2024

AbstractThe mechanism and predictive biomarkers of sinonasal inverted papilloma (IP) transformation into squamous cell carcinoma (SCC) are still unclear. We investigated the genetic mutations involved and the predictive biomarkers. Fourteen patients with SCC arising from IP and six patients with IPs without malignant transformation (sIP) were included. DNA was extracted separately from areas of normal tissue, IP, dysplasia, and SCC. Whole exome sequencing and immunohistochemistry was performed. Major oncogenic mutations were observed in the progression from IP to SCC. The most frequently mutated genes were TP53 (39%) and CDKN2A (27%). Mutations in TP53 and/or CDKN2A were observed in three of six IPs with malignant transformation (cIP); none were observed in sIPs. Tumor mutational burden (TMB) increased from IP to SCC (0.64/Mb, 1.11/Mb, and 1.25 for IP, dysplasia, and SCC, respectively). TMB was higher in the cIPs than in the sIPs (0.64/Mb vs 0.3/Mb). Three cIPs showed a diffuse strong or null pattern in p53, and one showed a total loss of p16, a distinct pattern from sIPs. Our result suggests that TP53 and CDKN2A status can be predictive markers of malignant transformation of IP. Furthermore, immunohistochemistry of p53 and p16 expression can be surrogate markers for TP53 and CDKN2A status.
Identification and analyses of exonic and copy number variants in spastic paraplegia
Authors: Shafique Anum , Nadeem Ayesha , Aslam Faiza , Manzoor Humera , Noman Muhammad , Wohler Elizabeth , Witmer P. Dane , Sobreira Nara , Naz Sadaf 
Published: June 2024

AbstractHereditary spastic paraplegias are a diverse group of degenerative disorders that are clinically categorized as isolated; with involvement of lower limb spasticity, or symptomatic, where spastic paraplegia is complicated by further neurological features. We sought to identify the underlying genetic causes of these disorders in the participating patients. Three consanguineous families with multiple affected members were identified by visiting special schools in the Punjab Province. DNA was extracted from blood samples of the participants. Exome sequencing was performed for selected patients from the three families, and the data were filtered to identify rare homozygous variants. ExomeDepth was used for the delineation of the copy number variants. All patients had varying degrees of intellectual disabilities, poor speech development, spasticity, a wide-based gait or an inability to walk and hypertonia. In family RDHR07, a homozygous deletion involving multiple exons and introns of SPG11 (NC000015.9:g.44894055_449028del) was found and correlated with the phenotype of the patients who had spasticity and other complex movement disorders, but not those who exhibited ataxic or indeterminate symptoms as well. In families ANMD03 and RDFA06, a nonsense variant, c.985C > T;(p.Arg329Ter) in DDHD2 and a frameshift insertion‒deletion variant of AP4B1, c.965-967delACTinsC;p.(Tyr322SerfsTer14), were identified which were homozygous in the patients while the obligate carriers in the respective pedigrees were heterozygous. All variants were ultra-rare with none, or very few carriers identified in the public databases. The three loss of function variants are likely to cause nonsense-mediated decay of the respective transcripts. Our research adds to the genetic variability associated with the SPG11 and AP4B1 variants and emphasizes the genetic heterogeneity of hereditary spastic paraplegia.
Partner gaze shapes the relationship between symptoms of psychopathology and interpersonal coordination
Authors: Macpherson M. C. , Brown A. J. , Kallen R. W. , Richardson M. J. , Miles L. K. 
Published: June 2024

AbstractInterpersonal coordination is a key determinant of successful social interaction but can be disrupted when people experience symptoms related to social anxiety or autism. Effective coordination rests on individuals directing their attention towards interaction partners. Yet little is known about the impact of the attentional behaviours of the partner themselves. As the gaze of others has heightened salience for those experiencing social anxiety or autism, addressing this gap can provide insight into how symptoms of these disorders impact coordination. Using a novel virtual reality task, we investigated whether partner gaze (i.e., direct vs. averted) influenced the emergence of interpersonal coordination. Results revealed: (i) spontaneous coordination was diminished in the averted (cf. direct) gaze condition; (ii) spontaneous coordination was positively related to symptoms of social anxiety, but only when partner gaze was averted. This latter finding contrasts the extant literature and points to the importance of social context in shaping the relationship between symptoms of psychopathology and interpersonal coordination.