bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natropharmacoalumite, NaAl4[(OH)4(AsO4)3].4H2O, a new mineral of the pharmacosiderite supergroup and the renaming of aluminopharmacosiderite to pharmacoalumite
Authors: Rumsey M. S. , Mills S. J. , Spratt J. 
Published: December 2010

AbstractNatropharmacoalumite, ideally NaAl4[(OH)4(AsO4)3]·4H2O, is a new mineral from the Maria Josefa Gold mine, Rodalquilar, Andalusia region, Spain. It occurs as colourless, intergrown cubic crystals with chenevixite, kaolinite, jarosite and indeterminable mixtures of Fe and Sb oxyhydroxides. Individual crystals are up to 0.5 mm on edge, although crystals are more commonly ˜0.25 mm across and occur in patchy aggregates several millimetres across. The mineral is transparent with a vitreous to adamantine lustre. It is brittle with an imperfect cleavage, irregular fracture and a white streak. The Mohs hardness is ˜2.5 with a calculated densityof 2.56 g cm–3 for the empirical formula. Electron microprobe analyses yielded Na2O 2.52%, K2O 1.49%, Al2O3 29.50%, As2O5 48.84% and H2O was calculated in line with the structural analysis as 16.28% totalling 98.63%. The empirical formula, based upon 20.21 oxygen atoms, is [Na0.57K0.22(H3O)0.21]Σ1.00Al4.05(As2.97O12)(OH)4·4H2O. The five strongest lines in the X-ray powder diffraction pattern are [dobs(Å), Iobs,(hkl)]: 7.759,100,(100); 4.473,40,(111); 3.870,50,(200); 2.446,9,(301); 2.331,12,(311). Natropharmacoalumite is cubic, space group with a = 7.7280(3) Å, V = 461.53(3) Å3 and Z = 1. The crystal structure was solved by direct methods and refined to R1 = 0.063 for 295 reflections with F>4σ(F). The structure conforms broadly to that of the general pharmacosiderite structure type, with Na as the dominant cation in cavities of strongly distorted Al octahedra and As tetrahedra. A new group nomenclature system for minerals with the pharmacosiderite structure has been established, including the renaming of aluminopharmacosiderite to pharmacoalumite.
RUNX1 mutations are frequent in de novo AML with noncomplex karyotype and confer an unfavorable prognosis
Authors: Schnittger Susanne , Dicker Frank , Kern Wolfgang , Wendland Nicole , Sundermann Jana , Alpermann Tamara , Haferlach Claudia , Haferlach Torsten 
Published: December 2010

Abstract
Analyses of 164 RUNX1 mutations (RUNX1mut) in 147 of 449 patients (32.7%) with normal karyotype or noncomplex chromosomal imbalances were performed. RUNX1mut were most frequent in acute myeloid leukemia French-American-British classification M0 (65.2%) followed by M2 (32.4%) and M1 (30.2%). Considering cytogenetics, RUNX1mut were most frequent in cases with +13 (27 of 30, 90%), whereas frequencies were similar in other cytogenetic groups (26%-36%). The molecular genetic markers most frequently associated with RUNX1mut were partial tandem duplication in the MLL gene (19.7%), internal tandem duplication in the FLT3 gene (FLT3-ITD; 16.3%), and NRAS mutations (9.5%). Patients with RUNX1mut had shorter overall and event-free survival compared with RUNX1 wild-type cases (median, 378 days vs not reached, P = .003; and median, 285 vs 450 days, P = .003, respectively). In addition, it was shown that the adverse effect of RUNX1 was independent of the adverse effect of FLT3-ITD as well as of the high frequency of prognostically favorable NPM1mut and CEBPAmut in the RUNX1wt group. No effect of the type or localization of the individual RUNX1 mutations was observed. Multivariate analysis showed independent prognostic relevance for overall survival for RUNX1mut (P = .029), FLT3-ITD (P = .003), age (P < .001), and white blood cell count (P < .002).
Comparison of treatment strategies for patients with intestinal diffuse large B-cell lymphoma: surgical resection followed by chemotherapy versus chemotherapy alone
Authors: Kim Seok Jin , Kang Hye Jin , Kim Jin Seok , Oh Sung Yong , Choi Chul Won , Lee Soon Il , Won Jong Ho , Kim Min Kyoung , Kwon Jung Hye , Mun Yeung-Chul , Kwak Jae-Yong , Kwon Jung Mi , Hwang In Gyu , Kim Hyo Jung , Park Jinny , Oh Sukjoong , Huh Jooryung , Ko Young Hyeh , Suh Cheolwon , Kim Won Seog 
Published: December 2010

Abstract
The aim of this retrospective cohort study was to analyze the impact of surgery on the outcomes and qualities of life (QOL) in patients with intestinal diffuse large B-cell lymphoma (DLBCL). We assessed 345 patients with either localized or disseminated intestinal DLBCL and compared them according to treatment: surgical resection followed by chemotherapy versus chemotherapy alone. In patients with localized disease (Lugano stage I/II), surgery plus chemotherapy yielded a lower relapse rate (15.3%) than did chemotherapy alone (36.8%, P < .001). The 3-year overall survival rate was 91% in the surgery plus chemotherapy group and 62% in the chemotherapy-alone group (P < .001). The predominant pattern in the chemotherapy group was local relapse (27.6%). When rituximab was used with cyclophosphamide, doxorubicin, vincristine, and prednisolone (CHOP), there was no improvement of the outcomes in patients treated with primary surgical resection. The QOL of patients who underwent surgery and chemotherapy was lower than chemotherapy alone, but its difference was acceptable. Multivariate analysis showed that surgical resection plus chemotherapy was an independent prognostic factor for overall survival. Surgical resection followed by chemotherapy might be an effective treatment strategy with acceptable QOL deterioration for localized intestinal DLBCL. This study was registered at www.clinicaltrials.gov as #NCT01043302.
Antiviral and lung protective activity of a novel respiratory syncytial virus fusion inhibitor in a mouse model
Authors: Olszewska W. , Ispas G. , Schnoeller C. , Sawant D. , Van de Casteele T. , Nauwelaers D. , Van Kerckhove B. , Roymans D. , De Meulder M. , Rouan M.C. , Van Remoortere P. , Bonfanti J.F. , Van Velsen F. , Koul A. , Vanstockem M. , Andries K. , Sowinski P. , Wang B. , Openshaw P. , Verloes R. 
Published: December 2010

Respiratory syncytial virus (RSV) causes bronchiolitis in young children and common colds in adults. There is no licensed vaccine, and prophylactic treatment with palivizumab is very expensive and limited to high-risk infants. Ribavirin is used as an antiviral treatment in infants and immunosuppressed patients, and its use is limited due to side-effects, toxicity to the recipient and staff, and evidence of marginal clinical efficacy. Therefore, we studied thein vivokinetics, and the antiviral and protective properties of a novel candidate for RSV disease treatment.The drug is a small molecule (TMC353121) discovered by screening for fusion inhibitory properties against RSV in a cellular infection model. The pharmacokinetics of TMC353121 was studied in BALB/c mice and antiviral effects determined by testing viral loads in lung tissue by quantitative RT-PCR and plaque assay after intranasal RSV infection.At doses of 0.25–10 mg·kg−1, TMC353121 significantly reduced viral load, bronchoalveolar lavage cell accumulation and the severity of lung histopathological change after infection. Treatment remained effective if started within 48 h of infection, but was ineffective thereafter.Therefore, TMC353121 is a novel potent antiviral drug,in vivoreducing RSV replication and inhibiting consequential lung inflammation, with a great potential for further clinical development.
Mast cells promote lung vascular remodelling in pulmonary hypertension
Authors: Hoffmann J. , Yin J. , Kukucka M. , Yin N. , Saarikko I. , Sterner-Kock A. , Fujii H. , Leong-Poi H. , Kuppe H. , Schermuly R.T. , Kuebler W.M. 
Published: December 2010

Left heart disease (LHD) frequently causes lung vascular remodelling and pulmonary hypertension (PH). Yet pharmacological treatment for PH in LHD is lacking and its pathophysiological basis remains obscure. We aimed to identify candidate mechanisms of PH in LHD and to test their relevance and therapeutic potential.In rats, LHD was induced by supracoronary aortic banding. Whole genome microarray analyses were performed, candidate genes were confirmed by RT-PCR and Western blots and functional relevance was testedin vivoby genetic and pharmacological strategies.In lungs of LHD rats, mast cell activation was the most prominently upregulated gene ontology cluster. Mast cell gene upregulation was confirmed at RNA and protein levels and remodelled vessels showed perivascular mast cell accumulations. In LHD rats treated with the mast cell stabiliser ketotifen, or in mast cell deficientWs/Wsrats, PH and vascular remodelling were largely attenuated. Both strategies also reduced PH and vascular remodelling in monocrotaline-induced pulmonary arterial hypertension, suggesting that the role of mast cells extends to non-cardiogenic PH.In PH of different aetiologies, mast cells accumulate around pulmonary blood vessels and contribute to vascular remodelling and PH. Mast cells and mast cell-derived mediators may present promising targets for the treatment of PH.
Time interval to conversion of interferon-γ release assay after exposure to tuberculosis
Authors: Lee S.W. , Oh D.K. , Lee S.H. , Kang H.Y. , Lee C-T. , Yim J-J. 
Published: December 2010

The proper interval for repeating an interferon-γ release assay (IGRA) among tuberculosis contacts with initially negative results is unknown. The interval for IGRA conversion after exposure to patients with active pulmonary tuberculosis in an outbreak setting was evaluated.In a platoon of 32 soldiers, four active pulmonary tuberculosis patients, in addition to one index patient, were diagnosed during a contact investigation. For the other 27 contacts, a tuberculin skin test (TST) and QuantiFERON®TB-Gold In-Tube (QFT-GIT) assay were performed. For soldiers with a negative result on the initial QFT-GIT assay, the test was repeated at 2, 4, 8, 14, 18 and 30 weeks until positive conversion occurred. When conversion was identified, the subject was treated for latent tuberculosis infection.Initially, 17 (63.0%) soldiers gave positive QFT-GIT results, whereas 21 (77.8%) showed positive TST results. Among 10 participants with initially negative QFT-GIT results, three showed conversion at 2 weeks, three at 4 weeks and three at 14 weeks. Conversion did not occur during the 30-week observation period in one contact.Based on the tuberculosis exposure time-points among the contacts, IGRA conversion generally occurred 4–7 weeks after exposure, although it could occur as late as 14–22 weeks after exposure.
Long-term effects of lung cancer computed tomography screening on health-related quality of life: the NELSON trial
Authors: van den Bergh K.A.M. , Essink-Bot M.L. , Borsboom G.J.J.M. , Scholten E.T. , van Klaveren R.J. , de Koning H.J. 
Published: December 2010

The long-term effects of lung cancer computed tomography (CT) screening on health-related quality of life (HRQoL) have not yet been investigated.In the Dutch–Belgian Randomised Lung Cancer Screening Trial (NELSON trial), 1,466 participants received questionnaires before randomisation (T0), 2 months after baseline screening (screen group only; T1) and at 2-yr follow-up (T2). HRQoL was measured as generic HRQoL (12-item short-form questionnaire and EuroQoL questionnaire), anxiety (Spielberger State-Trait Anxiety Inventory) and lung cancer-specific distress (impact of event scale (IES)). Repeated measures of ANOVA were used to analyse differences between the screen and control groups, and between indeterminate (requiring a follow-up CT) and negative screening result groups.At T0 and T2 there were no significant differences in HRQoL scores over time between the screen and control groups, or between the indeterminate or negative second-round screening result group. There was a temporary increase in IES scores after an indeterminate baseline result (T0: mean 4.0 (95% CI 2.8–5.3); T1: mean 7.8 (95% CI 6.5–9.0); T2: mean 4.5 (95% CI 3.3–5.8)).At 2-yr follow-up, the HRQoL of screened subjects was similar to that of control subjects, the unfavourable short-term effects of an indeterminate baseline screening result had resolved and an indeterminate result at the second screening round had no impact on HRQoL.
IFN-γ response on T-cell based assays in HIV-infected patients for detection of tuberculosis infection
Authors: Latorre Irene , Martínez-Lacasa Xavier , Font Roser , Lacoma Alicia , Puig Jordi , Tural Cristina , Lite Josep , Prat Cristina , Cuchi Eva , Ausina Vicente , Domínguez Jose 
Published: December 2010

Abstract

Background
Individuals infected with human immunodeficiency virus (HIV) have an increased risk of progression to active tuberculosis following Mycobacterium tuberculosis infection. The objective of the study was to determine IFN-γ responses for the detection of latent tuberculosis infection (LTBI) with QuantiFERON-TB GOLD In Tube (QFT-G-IT) and T-SPOT.TB in HIV patients, and evaluate the influence of CD4 cell count on tests performance.

Methods
We studied 75 HIV patients enrolled for ongoing studies of LTBI with T-SPOT.TB, QFN-G-IT and TST. Mean CD4 cell counts ± standard deviation was 461.29 ± 307.49 cells/μl. Eight patients had a BCG scar.

Results
T-SPOT.TB, QFN-G-IT and TST were positive in 7 (9.3%), 5 (6.7%) and 9 (12%) cases, respectively. Global agreement between QFN-G-IT and T-SPOT.TB was 89% (κ = 0.275). The overall agreement of T-SPOT.TB and QFN-G-IT with TST was 80.8% (κ = 0.019) and 89% (κ = 0.373), respectively. We have found negative IFN-γ assays results among 2 BCG-vaccinated HIV-infected individuals with a positive TST. In non BCG-vaccinated patients, QFN-G-IT and TST were positive in 5 cases (7.5%) and T-SPOT.TB in 7 (10.4%). In contrast, in BCG-vaccinated patients, only TST was positive in 4/8 (50%) of the cases. The differences obtained in the number of positive results between TST and both IFN-γ assays in BCG vaccinated patients were significant (95% CI 3-97%, p = 0.046), however, the confidence interval is very wide given the small number of patients. In patients with CD4< 200, we obtained only one (5%) positive result with T-SPOT.TB; however, QFN-G-IT and TST were negative in all cases. On the contrary, percentages of positive results in patients with CD4> 200 were 10.9% (6/55), 9.1% (5/55) and 16.4% (9/55) with T-SPOT.TB, QFN-G-IT and TST, respectively.

Conclusions
IFN-γ tests have the benefit over TST that are less influenced by BCG vaccination, consequently they are more specific than TST. Although our number of patients with advance immunosuppression is limited, our study suggests that IFN-γ assays are influenced with level of immunosuppression. The use of IFN-γ assays could be a helpful method for diagnosing LTBI in HIV population.
Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl
Authors: Chang Ing-Feng , Chen Peng-Jen , Shen Chin-Hui , Hsieh Tsung-Ju , Hsu Ya-Wen , Huang Bau-Lian , Kuo Ching-I , Chen Yu-Ting , Chu Hsiu-An , Yeh Kai-Wun , Huang Li-Chun 
Published: December 2010

AbstractBackgroundRestoration of rooting competence is important for rejuvenation inSequoia sempervirens(D. Don) Endl and is achieved by repeatedly graftingSequoiashoots after 16 and 30 years of cultivationin vitro.ResultsMass spectrometry-based proteomic analysis revealed three proteins that differentially accumulated in different rejuvenation stages, including oxygen-evolving enhancer protein 2 (OEE2), glycine-rich RNA-binding protein (RNP), and a thaumatin-like protein. OEE2 was found to be phosphorylated and a phosphopeptide (YEDNFDGNSNVSVMVpTPpTDK) was identified. Specifically, the protein levels of OEE2 increased as a result of grafting and displayed a higher abundance in plants during the juvenile and rejuvenated stages. Additionally,SsOEE2displayed the highest expression levels inSequoiashoots during the juvenile stage and less expression during the adult stage. The expression levels also steadily increased during grafting.ConclusionOur results indicate a positive correlation between the gene and protein expression patterns ofSsOEE2and the rejuvenation process, suggesting that this gene is involved in the rejuvenation ofSequoia sempervirens.
Characterization of influenza virus sialic acid receptors in minor poultry species
Authors: Kimble Brian , Nieto Gloria Ramirez , Perez Daniel R 
Published: December 2010

Abstract
It is commonly accepted that avian influenza viruses (AIVs) bind to terminal α2,3 sialic acid (SA) residues whereas human influenza viruses bind to α2,6 SA residues. By a series of amino acid changes on the HA surface protein, AIVs can switch receptor specificity and recognize α2,6 SA positive cells, including human respiratory epithelial cells. Animal species, like pigs and Japanese quail, that contain both α2,3 and α2,6 SA become ideal environments for receptor switching. Here, we describe the SA patterns and distributions in 6 common minor domestic poultry species: Peking duck, Toulouse geese, Chinese ring-neck pheasant, white midget turkey, bobwhite quail, and pearl guinea fowl. Lectins specific to α2,3 and α2,6 SA (Maakia amurensis agglutinin and Sambuca nigra agglutinin, respectively) were used to detect SA by an alkaline phosphotase-based method and a fluorescent-based method. Differences in SA moieties and their ability to bind influenza viruses were visualized by fluorescent labeling of 4 different H3N2 influenza viruses known to be specific for one receptor or the other. The geese and ducks showed α2,3 SA throughout the respiratory tract and marginal α2,6 SA only in the colon. The four other avian species showed both α2,3 and α2,6 SA in the respiratory tract and the intestines. Furthermore, the turkey respiratory tract showed a positive correlation between age and α2,6 SA levels. The fact that these birds have both avian and human flu receptors, combined with their common presence in backyard farms and live bird markets worldwide, mark them as potential mixing bowl species and necessitates improved surveillance and additional research about the role of these birds in influenza host switching.
Temporomandibular Joint Inflammation Activates Glial and Immune Cells in Both the Trigeminal Ganglia and in the Spinal Trigeminal Nucleus
Authors: Villa Giovanni , Ceruti Stefania , Zanardelli Matteo , Magni Giulia , Jasmin Luc , Ohara Peter T , Abbracchio Maria P 
Published: December 2010

Background:Glial cells have been shown to directly participate to the genesis and maintenance of chronic pain in both the sensory ganglia and the central nervous system (CNS). Indeed, glial cell activation has been reported in both the dorsal root ganglia and the spinal cord following injury or inflammation of the sciatic nerve, but no data are currently available in animal models of trigeminal sensitization. Therefore, in the present study, we evaluated glial cell activation in the trigeminal-spinal system following injection of the Complete Freund's Adjuvant (CFA) into the temporomandibular joint, which generates inflammatory pain and trigeminal hypersensitivity.Results:CFA-injected animals showed ipsilateral mechanical allodynia and temporomandibular joint edema, accompanied in the trigeminal ganglion by a strong increase in the number of GFAP-positive satellite glial cells encircling neurons and by the activation of resident macrophages. Seventy-two hours after CFA injection, activated microglial cells were observed in the ipsilateral trigeminal subnucleus caudalis and in the cervical dorsal horn, with a significant up-regulation of Iba1 immunoreactivity, but no signs of reactive astrogliosis were detected in the same areas. Since the purinergic system has been implicated in the activation of microglial cells during neuropathic pain, we have also evaluated the expression of the microglial-specific P2Y12receptor subtype. No upregulation of this receptor was detected following induction of TMJ inflammation, suggesting that any possible role of P2Y12in this paradigm of inflammatory pain does not involve changes in receptor expression.Conclusions:Our data indicate that specific glial cell populations become activated in both the trigeminal ganglia and the CNS following induction of temporomandibular joint inflammation, and suggest that they might represent innovative targets for controlling pain during trigeminal nerve sensitization.
Unusual histological findings after partial pancreaticoduodenectomy including benign multicystic mesothelioma, adenomyoma of the ampulla of Vater, and undifferentiated carcinoma, sarcomatoid variant: a case series
Authors: Lehwald Nadja , Cupisti Kenko , Baldus Stephan E , Kröpil Patric , Schulte am Esch Jan , Eisenberger Claus F , Knoefel Wolfram T 
Published: December 2010

Abstract

Introduction
The standard operation for carcinoma of the pancreatic head is a partial pancreaticoduodenectomy. Unusual histological findings may occasionally occur in the surgical specimen. We present three unusual histologic diagnoses after pancreaticoduodenectomy.

Case presentations
In the first case, an 86-year-old Caucasian woman was admitted with abdominal pain and nausea. Preoperative evaluation showed a 3 cm cystic lesion in the head of the pancreas. Pathology revealed a benign multicystic mesothelioma. In the second case, a 45-year-old Caucasian man complained of nausea, vomiting and general malaise for several months. Endoscopic retrograde cholangiopancreatographic examination and a computed tomography scan showed a stenosis of the distal bile duct secondary to a mass in the head of the pancreas and duodenum. Histology showed an adenomyoma of the ampulla. In the third case, a 59-year-old Caucasian man presented with chronic alcoholic pancreatitis. A computed tomography scan revealed a 3.5 cm lesion in the head of the pancreas with cystic and solid components. Pathology showed an undifferentiated carcinoma, sarcomatoid variant.

Conclusion
Partial pancreaticoduodenectomy is usually performed for ductal adenocarcinomas, neuroendocrine tumors or chronic pancreatitis. Compared to the majority of the above diagnoses, the three cases in our study are very rare. Benign multicystic mesothelioma is a very rare tumor that originates from the peritoneum. Although it demonstrates a benign clinical behaviour, it frequently recurs after resection. Adenomyoma of the bile duct or ampullary region is a very unusual, benign, localized lesion characterized by adenomyomatous hyperplasia. Undifferentiated carcinoma, sarcomatoid variant, is an aggressive tumor and is characterized by spindle cells. As the lesions were suspicious for carcinoma, partial pancreaticoduodenectomy was justified in all three patients. The histologic diagnosis after partial pancreaticoduodenectomy may differ from the preoperative and intraoperative findings. These cases demonstrate that a definitive diagnosis may only be obtained by a pathologic examination of the surgical specimen.
High-dose steroid therapy for idiopathic optic perineuritis: a case series
Authors: Tatsugawa Maria , Noma Hidetaka , Mimura Tatsuya , Funatsu Hideharu 
Published: December 2010

Abstract

Introduction
It has been reported that the prognosis of optic perineuritis may be poor when initiation of treatment is delayed. Here we report the successful treatment of three patients with idiopathic optic perineuritis, including two in whom initiation of therapy was delayed.

Case presentation
Three Japanese patients (two women aged 73 and 66 years, and one man aged 27 years) presented with loss of vision (for five months, several months, and two months respectively) and pain on eye movement in the third case only, and were diagnosed as having idiopathic optic perineuritis. Fat-suppressed T2-weighted magnetic resonance images showed high signal intensity areas around the affected optic nerves, suggesting the presence of optic perineuritis. Two patients received steroid pulse therapy and the third was given high-dose steroid therapy. The visual acuity improved in all three cases.

Conclusion
High-dose steroid therapy may be effective for idiopathic perineuritis in patients without optic nerve atrophy, even if initial treatment (including moderate-dose steroids) has failed.