bluuebunny/crossref_metadata_2025_split huggingface dataset:
Common food additive carrageenan inhibits proglucagon expression and GLP-1 secretion by human enteroendocrine L-cells
Authors: Bhattacharyya Sumit , Borthakur Alip , Tobacman Joanne K. 
Published: May 2024

AbstractProglucagon mRNA expression and GLP-1 secretion by cultured human L-cells (NCI-H716) were inhibited following exposure to λ-carrageenan, a commonly used additive in processed foods. Carrageenan is composed of sulfated or unsulfated galactose residues linked in alternating alpha-1,3 and beta-1,4 bonds and resembles the endogenous sulfated glycosaminoglycans. However, carrageenan has unusual alpha-1,3-galactosidic bonds, which are not innate to human cells and are implicated in immune responses. Exposure to carrageenan predictably causes inflammation, and carrageenan impairs glucose tolerance and contributes to insulin resistance. When cultured human L-cells were deprived overnight of glucose and serum and then exposed to high glucose, 10% FBS, and λ-carrageenan (1 µg/ml) for 10 minutes, 1 h, and 24 h, mRNA expression of proglucagon and secretion of GLP-1 were significantly reduced, compared to control cells not exposed to carrageenan. mRNA expression of proglucagon by mouse L-cells (STC-1) was also significantly reduced and supports the findings in the human cells. Exposure of co-cultured human intestinal epithelial cells (LS174T) to the spent media of the carrageenan-treated L-cells led to a decline in mRNA expression of GLUT-2 at 24 h. These findings suggest that ingestion of carrageenan-containing processed foods may impair the production of GLP-1, counteract the effect of GLP-1 receptor agonists and induce secondary effects on intestinal epithelial cells.
MiR-1976/NCAPH/P65 axis inhibits the malignant phenotypes of lung adenocarcinoma
Authors: Huang Peiluo , Zhao Hongtao , Sun Ruonan , Liu Chunyan , Wu Lei , Wang Yao , Gan Zhengwei , Yang Xiuzhen , Du Juan 
Published: May 2024

AbstractLung adenocarcinoma (LUAD) is a malignancy with an abysmal survival rate. High metastasis is the leading cause of the low survival rate of LUAD. NCAPH, an oncogene, is involved in the carcinogenesis of LUAD. However, the regulation of NCAPH in LUAD remains controversial. In this work, we identified an up-regulation of NCAPH in LUAD tissues. Patients who expressed more NCAPH had shorter overall survival (OS). Furthermore, NCAPH overexpression promoted LUAD cell migration while inhibiting apoptosis. MiR-1976 and miR-133b were predicted to target NCAPH expression by searching TargetScan and linkedomics databases. Following that, we confirmed that miR-1976 suppressed NCAPH by directly targeting a 7-bp region of NCAPH 3′ untranslated regions (UTR). In addition, increased expression of miR-1976 decreased the proliferation & migration and promoted apoptosis of LUAD cells, and the re-introduction of NCAPH reversed these influences. Furthermore, the xenograft and metastasis mouse models also confirmed that miR-1976 inhibited tumor growth and metastasis in vivo by targeting NCAPH. Finally, we found that MiR-1976 targeting NCAPH blocked the activation of NF-κB. In conclusion, miR-1976 inhibits NCAPH activity in LUAD and acts as a tumor suppressor. The miR-1976/NCAPH/NF-κB axis may, in the future, represent crucial diagnostic and prognostic biomarkers and promising therapeutic options.
The miR-641-STIM1 and SATB1 axes play important roles in the regulation of the Th17/Treg balance in ITP
Authors: Zhu Hongkai , Ruan Xueqin , Zhao Kexin , Kuang Wenyong , Liu Sufang , Yan Wenzhe , Fu Xianming , Cheng Zhao , Li Ruijuan , Peng Hongling 
Published: May 2024

AbstractImmune thrombocytopenia (ITP) is an autoimmune disease caused by T-cell dysfunction. Recently, several studies have shown that a disturbed Th17/Treg balance contributes to the development of ITP. MicroRNAs (miRNAs) are small noncoding RNA moleculesthat posttranscriptionally regulate gene expression. Emerging evidences have demonstrated that miRNAs play an important role in regulating the Th17/Treg balance. In the present study, we found that miR-641 was upregulated in ITP patients. In primary T cells, overexpression of miR-641 could cause downregulation of its target genes STIM1 and SATB1, thus inducing a Th17 (upregulated)/Treg (downregulated) imbalance. Inhibition of miR-641 by a miR-641 sponge in primary T cells of ITP patients or by antagomiR-641 in an ITP murine model could cause upregulation of STIM1 and SATB1, thus restoring Th17/Treg homeostasis. These results suggested that the miR-641-STIM/SATB1 axis plays an important role in regulating the Th17/Treg balance in ITP.
CRTAC1 identified as a promising diagnosis and prognostic biomarker in lung adenocarcinoma
Authors: Tan Lin , Zhang Han , Ding Yun , Huang Yangyun , Sun Daqiang 
Published: May 2024

AbstractCRTAC1, one of the pyroptosis-related genes, has been identified as a protective factor in certain kinds of cancer, such as gastric adenocarcinoma and bladder cancer. The study aimed to investigate the role of CRTAC1 in lung adenocarcinoma (LUAD). LUAD datasets were obtained from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA), pyroptosis-related genes from GeneCard. Limma package used to find differentially expressed genes (DEGs), least absolute shrinkage and selection operator (LASSO) regression and weighted genes co-expression network analysis (WGCNA) to identify CRTAC1 as hub gene. CRTAC1 expression was confirmed in a real-world cohort using quantitative polymerase chain reaction (qPCR) and Western Blot (WB) analyses. Cellular experiments were conducted to investigate CRTAC1’s potential oncogenic mechanisms. CRTAC1 mRNA expression was significantly lower in LUAD tissues (p < 0.05) and showed high accuracy in diagnosing LUAD. Reduced CRTAC1 expression was associated with a poor prognosis. Higher CRTAC1 expression correlated with increased immune cell infiltration. Individuals with high CRTAC1 expression showed increased drug sensitivity. Additionally, qPCR and WB analyses showed that CRTAC1 expression was lower in tumor tissue compared to adjacent normal tissue at both the RNA and protein levels. Upregulation of CRTAC1 significantly inhibited LUAD cell proliferation, invasion, and migration in cellular experiments. CRTAC1 has the potential to serve as a diagnostic and prognostic biomarker in LUAD.
A Picture May Be Worth 1,000 Words, but Is It Worth a Letter? Examining Whether the Choice of Label Affects the Perception of Speech Sounds
Authors: Levi Susannah V. , Cheng Hung-Shao , O'Brien Gabrielle , Harel Daphna 
Published: May 2024

Purpose:
Researchers often use identification or goodness rating tasks to assess speech perception for different populations. These tasks provide useful information about a listener's willingness to accept a range of acoustically variable stimuli as belonging to the same category and also about assessing how stimuli that are labeled the same may not be perceived as equally good versions of a particular speech sound. Many methodological aspects of these simple tasks have been tested, but one aspect that has not is the choice of label. In this study, we examine response patterns to images versus letters, as studies with different populations (children vs. adults) or different methods (typical behavioral study vs. visual world paradigm) may vary in the type of label used.

Method:
Eighty-one adult listeners completed phoneme identification and goodness ratings tasks with either images of response options (a picture of a bear and a picture of a pear) or with letter labels (a capital B and P).

Results:
The results suggest that choice of label does not alter performance within the tasks studied here. In addition, the results did show the expected finding that the slope of the response curve is steeper in an identification task than in a goodness rating task.

Conclusion:
These results suggest that it is possible to compare across studies that use different response options, a benefit to research and practice because letter labels can be used for nonimageable words and nonwords, whereas images may be best used for participants who are younger or have poorer reading skills.
Case study using APDMS and RPEMS for SAWL pipes and benefits for offshore pipelay
Authors: Kathayat T. S. , Srivastava Atul , de Boer Jan , Mapara Bhavin , Gajjar Mahesh 
Published: May 2024

This paper reviews an Automatic Pipe Dimension Measurement System (APDMS) and a Robotic Pipe End Measurement System (RPEMS) which can measure 19 and 9 parameters with the help of 72 and 6 measurement sensors respectively. APDMS and RPEMS are integrated into one optical measurement system, using laser triangulation, coupled with Systems, Applications and Products in Data Processing (SAP) integration and its own calibration modulus which provides an accurate solution to all pipe measurements. Two longitudinal submerged arc welded offshore projects were executed for an offshore pipe lay with close dimensional tolerances measured using the APDMS for one project and RPEMS for the other project. The radii data, assessed from the newly calculated common centre of all cross-sectional data, was used to line up the two adjacent pipes in a faster and more accurate way. By knowing the shape of the pipe ends based on this radii data, the best relative rotation of these two pipes can be determined and any misalignment will be verified and will stay within the requirements. The extensive database available for each pipe helped to match the line pipes with similar or close out-of-roundness at pipe ends resulting in good fitment of the pipes and ultimately quality welds, together with more pipe joints per day, resulting in a faster completion of the project. This paper shows the significance of using both systems.
Laparoscopic Magnetic Sphincter Augmentation for Symptomatic Gastroesophageal Reflux After Roux-en-Y Gastric Bypass: Outcome in 13 Patients
Authors: Moser Schaub E , Fringeli Y , Kessler U , Zehetner J 
Published: May 2024

Abstract

Background
Gastroesophageal reflux disease (GERD) represents a common burden after bariatric procedures, particularly after laparoscopic sleeve gastrectomy (LSG). While GERD after LSG is usually treated with conversion to Roux-en-Y gastric bypass (RYGB), there are limited surgical options to manage medically refractory GERD after RYGB due to the altered anatomy. The use of magnetic sphincter augmentation (MSA) is one option, but the literature on this topic remains scarce.

Aims
To present mid- to long-term outcome of patients treated with MSA for GERD after RYGB.

Methods
We performed a retrospective single-centre analysis of data from patients who underwent laparoscopic MSA for GERD between August 2015 and December 2023, and who had previously RYGB. Outcomes were persistence/recurrence of GERD symptoms, use of proton pump inhibitors (PPIs) and need for redo surgery related to MSA complications or recurrent GERD.

Results
Of the 312 MSA procedures performed for GERD, we identified 13 patients who underwent laparoscopic MSA after RYGB. There were 10 female patients (77%), with mean age of 42.6 ± 13.7 years and mean BMI of 27.2 ± 2.7 kg/m2. Seven patients (54%) had de novo GERD. All MSA procedures included hiatoplasty. Median follow-up was 40 months (range 7-72) and median time between RYGB and MSA placement was 42 months (range 8-242). One patient (8%) required endoscopic dilatation due to persistent postoperative dysphagia. Two patients (15%) needed redo surgery: 1 redo hiatoplasty with adhaesiolysis and 1 conversion to esophagojejunostomy with implant removal. There was no erosion or migration of the MSA implant. Overall satisfactory control of reflux with discontinuation or reduction of PPIs was achieved in 9 patients (69%).

Conclusion
The use of MSA to manage refractory GERD after RYGB is a safe option and was effective in 69% of patients but is limited to patients with intact oesophageal motility.
Robotic-Assisted Inguinal Hernia Repair with The Dexter System™: A Prospective Multicentre Clinical Investigation
Authors: Gantner L , Mignot H , Pochhammer J , Grieder F , Breitenstein S 
Published: May 2024

Abstract

Background
Robot-assisted transabdominal preperitoneal inguinal hernia repair (rTAPP) is established as safe and feasible with various robotic platforms. The Dexter robotic system is an open platform with a sterile surgeon’s console, two robotic instrument arms, and one robotic endoscope arm.

Aims
We conducted a prospective multicentre study (ClinicalTrials.gov NCT05873582) to assess the safety and performance of this new robotic platform.

Methods
Three centres in France, Germany, and Switzerland participated. The primary objectives of the study were to document the successful completion of the rTAPP procedures and to collect data on the occurrence of adverse events and device-related events perioperatively and up to 30 days post-surgery. The procedures were performed by three surgeons with varying levels of experience in robotic systems, including novice, intermediate and expert.

Results
Fifty patients were operated for inguinal hernia repair (33 unilateral, 17 bilateral). The mean age and BMI of the patients were 60 years (±15) and 25.7 kg/cm2 (±3.3), respectively. All surgeries were successfully completed as planned using three standard laparoscopy trocars sized 10/12mm. Each procedure was performed with robotic assistance, and there were no conversions to open surgery. No intra-operative complications, device deficiency, or adverse events of Clavien-Dindo Grade III to V occurred. The mean skin-to-skin operative time was 55 min (±14) for unilateral hernia repair and 95 min (±22) for bilateral hernia repair. The mean robotic console time was 34 min (±12) for unilateral hernia and 68 min (±21) for bilateral hernia repairs. The patients were discharged within 1day (± 1 day) after surgery.

Conclusion
Our rTAPP experience with the Dexter system confirms feasibility, safety, and comparable operative times to other robotic platforms. This study demonstrates the practicality and safety of the robotic techniques, positioning the Dexter system as a valuable tool in hernia repair.
Randomised Controlled Pilot Trial on GHOST Ileostomy versus Conventional Loop Ileostomy in Patients Undergoing low Anterior Resection for Rectal Cancer
Authors: Probst P , Klotz R , Diener M K , Knebel P , Hüttner F J 
Published: May 2024

Abstract

Background
Anastomotic leakage is the most important complication after colorectal surgery. In patients undergoing low anterior resection with total mesorectal excision (LAR/TME) a loop ileostomy is fashioned to protect the low anastomosis. Ghost ileostomy is a pre-stage ileostomy, which can be converted to a diverting ileostomy, if anastomotic leakage is suspected. If there are no signs of anastomotic leakage an actual ileostomy can be avoided.

Aims
The objective was to evaluate whether ghost ileostomy is a safe alternative to a conventional loop ileostomy.

Methods
GHOST was a randomised controlled pilot trial without formal hypothesis. Patients undergoing LAR/TME for rectal cancer were intraoperatively randomised to receive a ghost ileostomy or loop ileostomy. Patients were followed prospectively for 6 months. Comprehensive complication index, transformation of ghost ileostomy into loop ileostomy, presence of an ostomy at 6 months, Wexner score and quality of life were assessed.

Results
Thirty patients were equally randomised. The CCI was 30.7 (± 17.7) in the ghost ileostomy group compared to 29.7 (± 18.6) in the loop ileostomy group at 6 months (p=0.889). The ghost ileostomy was converted into a loop ileostomy in 6 of 15 (40.0%) patients. At 6 months after low anterior resection, 6 (40.0%) patients in the ghost ileostomy group and 7 (46.7%) patients in the ileostomy group still had an ileostomy. There was no mortality and no need for creation of a terminal ostomy within the trial. Neither postoperative function assessed by the Wexner score, nor the overall quality of life showed significant differences.

Conclusion
Ghost ileostomy seems to be a viable and safe option to a more selective approach regarding creation of an ileostomy in patients undergoing LAR/TME. However, the difficult patient selection with exclusion of patients at high risk of anastomotic leakage limits its widespread application and should be optimised in future trials.
Dysregulated MicroRNAs Contribute to Chemotherapy-Resistance of Pleural Mesothelioma
Authors: Kirschner M B , Schläpfer F , Meerang M , Opitz I 
Published: May 2024

Abstract

Background
Dysregulated expression of microRNAs has been shown to contribute to response to chemotherapeutic agents in several cancers, including pleural mesothelioma (PM). In previous work, we found expression of several tissue-microRNAs to correlate with patient’s response to cisplatin-pemetrexed.

Aims
We want to understand how altering the expression of these microRNAs affects cell growth and chemo response.

Methods
PM cell lines MSTO-211H (biphasic), H28, Meso-1, Mero-82 (epithelioid) and non-malignant MeT-5A were reverse transfected with synthetic microRNA mimics for 15 candidates. Following transfection, cell growth, colony forming ability, and migratory potential were assessed using standard assays. Furthermore, transfected cells were exposed to increasing concentrations of cisplatin to evaluate sensitivity to these drugs.

Results
Overexpression of seven candidate microRNAs resulted in growth inhibition in all investigated PM cell lines five days post transfection. The strongest effects were observed for miR-380-5p, miR-221-3p, miR-210, miR-625-3p, and miR-19b, which reduced cell growth to 30-60%. Growth of non-malignant MeT-5A cells remained largely unaffected (Fig. A). Overexpression of those microRNAs also resulted in a strong reduction of colony forming ability (Fig. B), while an effect on wound healing capacity (migration) could not be observed. Finally, we found that especially overexpression of miR-221-3p, miR-625-3p and miR-19b was able to sensitise cells towards cisplatin, with the strongest effect observed in MSTO-211H for miR-221-3p (IC50 from 17.6µM to 2.7µM) and miR-19b (IC50 from 17.6µM to 0.7µM), and in Mero-82 for miR-625-3p (IC50 from 3.75µM to 0.8µM) (Fig. C).

Conclusion
We show that overexpression of several microRNAs has the potential to alter PM cell growth and colony forming ability. Furthermore, microRNA overexpression can sensitise cells towards cisplatin, although the degree of sensitisation varies between different cell lines. Current analyses focus on the response towards the cisplatin/pemetrexed doublet and on the effect on cell cycle and expression of associated genes.
Accuracy in Navigated Percutaneous Sacroiliac Screw Fixation: A Systematic Review and Meta-Analysis
Authors: Haveman R , van de Wall B , Haefeli P , Beeres F , Babst R , Link B C 
Published: May 2024

Abstract

Background
Percutaneous sacroiliac screw placement is a challenging procedure in patients with pelvic fractures. To overcome these challenges, navigated techniques have emerged as an alternative to conventional 2D fluoroscopy for guiding screw placement. However, it remains to be seen whether navigated techniques truly have a beneficial effect on accuracy, radiation exposure and occurrence of complications.

Aims
We performed a meta-analysis to investigate whether navigated techniques are superior to conventional 2D fluoroscopy in percutaneous sacroiliac screw fixation.

Methods
The electronic databases were searched for both randomized clinical trials and observational studies comparing percutaneous sacroiliac screw fixation with 2D fluoroscopy to new navigated techniques (2D or 3D fluoroscopic navigation with a c-arm, computer assisted ultrasound navigation, O-Arm navigation, mobile CT navigation, robotic navigation). Effects were pooled and presented as odds ratio, mean difference, and standardized mean difference with corresponding 95% confidence interval.

Results
In total 18 studies were included. New navigated techniques had a higher accuracy (81% versus 91% with MD 10%, 95% CI 5-14%). The greatest benefit in accuracy was found in studies with more advanced navigated techniques (O-Arm, mobile CT or robotic navigation). Also, fluoroscopy time (MD 72.13 seconds, 95% CI 7.73;92.91) and fluoroscopy frequency (MD 17.22 images in total, 95% CI 7.73;26.70) were lower for new navigated techniques. Radiation dose was higher for new navigated techniques (SMD 0.50, 95% CI 0.01;0.99). Surgery duration showed no significant difference. Similar results were found among in vitro studies. Complications were rare in both groups.

Conclusion
This meta-analysis demonstrated a higher accuracy of screw positioning, lower fluoroscopic frequency and time for navigated percutaneous sacroiliac screw fixation compared to conventional 2D fluoroscopy. Complications are acceptably low for both groups. Future studies should focus on which of the navigated techniques is the best and whether the implementation costs of a new technique outweigh its benefits.
LATE ONSET FABRY’S DISEASE MIMICKING HYPERTROPHIC CARDIOMYOPATHY
Authors: Allocca G , Centa M , Bagato F , Sitta N , Gallucci M , Carraro G , Vitturi N , De Michieli L , Rivetti L , Bullian F , Mantovan R 
Published: May 2024

Abstract
Fabry disease (FD) is rare genetic syndrome which determines a lysosomal storage disorder caused by pathogenic variants in the alpha–galactosidase A (GLA) gene that lead to alpha–galactosidase A (AGAL– A) enzyme activity disfunction.The fenotipic effect is a left ventricular hypertrophy (LVH) that is frequently misdiagnosed as hypertrophic cardiomyopathy (HCM).This disease in still unfamiliar for the most part of cardiologists cause of the fenotipic familiarities with other more common and wellknown diseases and according to recent data the prevalence of FD among unexplained LVH is 0.93%.These characteristics drive to a heavy under diagnosis of a disease with many therapy chances. The prompt initiation of these treatments, subsequently an early diagnosis, is actually considered the best strategy to improve the natural course of the disease.The critical evaluation of the clinical history and basic cardiomiopaty assessments could drive the cardiologist to the diagnosis.Precisely, we present an example of three family pedigree discovered in a couple of months subsequently the unmasking of proband male patients with decennary misdiagnosis of HCM. The ambulatory suspect of non sarcomeric disease in LVH drove us to critically reexaminate EKGs and previous CMR scans finding respectively PR shortage associated with right bundle block and basal inferolateral late gadolinium enhancement (figure 1). In a proband it is not possible to perform MRI with gadolinium due to the presence of renal failure and the diagnostic suspicion arose by analyzing the T1 mapping signal (figure 2).The score ID FABRY–HCM were applied resulting suggestive too.Having thus corroborated the suspicion, blood spot tests were performed finally detecting a very low AGAL–A activity. The following GLA genetic tests identified the tipical mutation of late onset Fabry’s disease (p.N215Ser). Fulfilling the consensus criteria for the therapy initiation, the patients were treated with enzyme replacement therapy. We completed families predigree identifying other six famale gene mutation carriers with almost preserved AGAL–A activity. The diagnosis of FD allowed us to perform the specific therapy in affected patients hopefully effecting their quality of life and their prognosis and to bring out healthy carriers.The initial suspect questioning a previous diagnosis starting to simple clinical and routine strumental elements were crucial.