bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemocompatibility of titania nanotube arrays
Authors: Smith Barbara S. , Yoriya Sorachon , Grissom Laura , Grimes Craig A. , Popat Ketul C. 
Published: July 2010

AbstractHemocompatibility is a key consideration for the long‐term success of blood contacting biomaterials; hence, there is a critical need to understand the physiological response elicited from blood/nano–biomaterial interactions. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets, and clotting kinetics of whole blood on titania nanotube arrays. Previous studies have demonstrated improved mesenchymal stem cell functionality, osteoblast phenotypic behavior, localized drug delivery, and the production of endothelial cell ECM on titania nanotube arrays. Furthermore, these titania nanotube arrays have elicited minimal levels of monocyte activation and cytokine secretion, thus exhibiting a very low degree of immunogenicity. Titania nanotube arrays were fabricated using anodization technique and the surface morphology was examined through scanning electron microscopy (SEM). The crystalline phases were identified using glancing angled X‐ray diffraction (GAXRD). Nanoindentation and scratch tests were used to characterize the mechanical properties of titania nanotube arrays. The adsorption of key blood proteins (albumin, fibrinogen, and immunoglobulin‐g) was evaluated using a micro‐BCA assay and X‐ray photoelectron spectroscopy (XPS). The adhesion and activation of platelets was investigated using live‐cell staining, MTT assay, and SEM. Whole blood clotting kinetics was evaluated by measuring the free hemoglobin concentration, and SEM was used to visualize the clot formation. Our results indicate increased blood serum protein adsorption, platelet adhesion and activation, and whole blood clotting kinetics on titania nanotube arrays. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Biological performance of biodegradable amino acid‐based poly(ester amide)s: Endothelial cell adhesion and inflammation in vitro
Authors: Horwitz Joshua A. , Shum Katrina M. , Bodle Josephine C. , Deng MingXiao , Chu Chih‐Chang , Reinhart‐King Cynthia A. 
Published: July 2010

AbstractFunctionalized amino‐acid‐based poly(ester‐amide)s (PEA) are a new family of synthetic biodegradable polymers consisting of three naturally occurring building blocks (amino acids, diols, and dicarboxylic acids) that have been suggested to be promising biomaterials for therapeutic use. However, little is known about their cytotoxicity, ability to support cell growth, inflammatory properties, or mechanical properties, key aspects to most biomaterials designed for in vivo implantation and tissue engineering applications. In this study, we investigated the ability of two functionalized PEA materials (amino‐functionalized and carboxylic acid functionalized) and a neutral PEA control to support endothelial cell viability, proliferation, and adhesion. Additionally, we investigated the inflammatory response elicited by these functionalized PEA materials using a macrophage cell model. Our results indicate that all forms of PEA were noncytotoxic and noninflammatory in vitro. The amino‐functionalized PEA bests supports endothelial cell adhesion, growth, and monolayer formation. Mechanical testing indicates that the elastic moduli of these materials are strongly dependent on the charge formulation, but do exhibit linearly elastic behavior at small strains (<10%). Our data suggest that PEA may be a viable biomaterial for use in tissue engineering applications, particularly for use as a vascular graft. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Serum prolidase activity in idiopathic and ischemic cardiomyopathy patients
Authors: Sezen Yusuf , Bas Memduh , Altıparmak Halil , Yildiz Ali , Buyukhatipoglu Hakan , Faruk Dag Omer , Kaya Zekeriya , Aksoy Nurten 
Published: July 2010

AbstractIdiopathic and ischemic dilated cardiomyopathies (DCM) are the most common types of DCM, and both exhibit the same histopathological feature of fibrosis. Prolidase is an enzyme that serves a rate‐limiting function in collagen turnover. Several studies have shown increased prolidase activity in fibrosis, though controversy persists. In this study, we measured prolidase enzyme activity in patients with idiopathic or ischemic DCM and in healthy controls, making this, to our knowledge, the first study to do so. What we found is that serumprolidase activity was significantly lower in both DCM groups relative to healthy volunteers and lower in ischemic DCM than idiopathic. These intriguing results could be attributed either to decreased collagen turnover in the heart tissues in which DCM develops, a result of diminished functional heart tissue, or to decreased physical activity levels among DCM patients stemming from their heart failure. Either way, further studies are needed to verify and clarify our results. J. Clin. Lab. Anal. 24:213–218, 2010. © 2010 Wiley‐Liss, Inc.
Rapid mycoplasma culture for the early diagnosis of Mycoplasma pneumoniae infection
Authors: Ma Ling‐di , Chen Baojin , Dong Yanfen , Fan Jing , Xia Lei , Wang Shi‐zhong , Liu Qian , Jiang Lijia 
Published: July 2010

AbstractEarly diagnosis of Mycoplasma pneumoniae (Mp) plays a pivotal role in its management. We evaluated the role of rapid culture method in early diagnosis of Mp infection and discussed the potential impact factors. A total of 2,600 patients with acute respiratory infection were included, and their pharyngeal swab samples were prepared for Mp rapid culture based on selective Mp fluid culture medium. The clinical contributing factors related to Mp infection were also explored. The positive rate of Mp culture in females was 41.75%, which was higher than that for males (37.63%). Mp infections were incidental to the children and elderly. The positive rates of Mp culture were higher in children aged 3–5 years and adults older than 70 years (54.05 and 31.48%, respectively), compared with other ages. In addition, Mp infection frequently occurred in winter (December–February) and spring (March–May), with significantly higher positive rates of Mp culture by 40.02 and 42.89 vs. 32.15 and 33.50% in summer (June–August) and autumn (September–November), respectively. The positive rate of rapid culture for Mp was slightly higher than that by Mp antibody assay, but the diagnostic accordance was well between these two methods (P>0.05). Furthermore, clinical common symptoms of respiratory tract infection, such as fever, cough, and expectoration, were not found specific in Mp infection, suggesting that they were not independent prognostic predictors for Mp infection. Therefore, rapid culture based on the Mp pathogen detection would have important clinical application for the early diagnosis of Mp infection. J. Clin. Lab. Anal. 24:224–229, 2010. © 2010 Wiley‐Liss, Inc.
Quantification of urinary 8‐iso‐prostaglandin F2αusing liquid chromatography–tandem mass spectrometry during cardiac valve surgery
Authors: Teng Yun‐Hui , Wang Chih‐Wei , Liao Yun‐Tsan , Yang Min‐Wen , Liu Tsung‐Yun 
Published: July 2010

AbstractOxidative stress is an unavoidable event during many complex surgical procedures. 8‐iso‐prostaglandin F2α(8‐iso‐PGF2α) is a reliable biomarker for the evaluation of oxidative stress in vivo. The aim of this study is to develop simple and accurate liquid chromatography–tandem mass spectrometry (LC‐MS/MS) methods for the detection of urinary 8‐iso‐PGF2αin samples collected from patients who received a cardiopulmonary bypass (CPB) during cardiac valve surgery. Urine samples of 14 patients with cardiac valve diseases were collected before, during, and after CPB. The level of 8‐iso‐PGF2αwas detected via selected‐reaction monitoring triple quadrupole MS/MS and the result was compared with 12 healthy volunteers. The method's detection limit (3S/N) was 0.25 pg for 8‐iso‐PGF2α, with a linear working range of 0.25–20 ng/ml. For patients with cardiac valve disease, the 8‐iso‐PGF2αlevels before the bypasses were the same as those of healthy individuals (P>0.05) and the 8‐iso‐PGF2αlevels during and after CPB were significantly higher than those before the bypasses (P<0.05). In conclusion, we present a simple and specific protocol for LC‐MS/MS quantification of urinary 8‐iso‐PGF2αcollected during CPB. Using this technique, it would be feasible to assess the levels of oxidative stress during cardiac surgery and thereby helpful for the management of oxidative injury. J. Clin. Lab. Anal. 24:237–245, 2010. © 2010 Wiley‐Liss, Inc.
Celiac disease‐associated antibodies in patients with psoriasis and correlation with HLA Cw6
Authors: Singh Sangeeta , Sonkar Gyanendra Kumar , Usha  , Singh Sanjay 
Published: July 2010

AbstractEtiopathology of psoriasis is not completely understood. Patients with psoriasis show elevated sensitivity to gluten. The aim of this study was to see the expression of celiac disease (CD)‐associated antibodies gliadin IgA, gliadin IgG, and tissue transglutaminase IgA, and their correlation with HLA Cw6 in patients with psoriasis. The study comprised 56 patients with psoriasis and 60 healthy controls (HC). The levels of antibodies were detected by using ELISA technique and HLA Cw6 typing was carried out by microcytotoxicity method. HLA Cw6 was significantly expressed in psoriasis cases when compared with HC (P<0.05). CD‐associated antibodies gliadin IgA/IgG and tissue transglutaminase IgA were significantly higher in the serum of patient with psoriasis when compared with HC (P<0.05, <0.05, and 0.01, respectively). Serum anti tissue transglutaminase IgA (anti tTG IgA) was significantly higher in females when compared with males and expressed more in elderly patients. There was a significant positive correlation among the antibodies (anti gliadin IgA with anti gliadin IgG: r=0.67, P<0.05; anti gliadin IgA with anti tTG IgA: r=0.45, P<0.05, anti gliadin IgG with anti tTG IgA: r=0.26, P<0.05, respectively), whereas insignificant with HLA Cw6. Our study concludes that latent CD or CD‐associated antibodies were present in patients with psoriasis and also concludes that HLA Cw6 has no association with expression of these antibodies in patients with psoriasis. J. Clin. Lab. Anal. 24:269–272, 2010. © 2010 Wiley‐Liss, Inc.
Ischemia‐Modified albumin levels in patients with end‐stage renal disease patients on hemodialysis: does albumin analysis method affect albumin‐adjusted Ischemia‐Modified albumin levels?
Authors: Kıyıcı Aysel , Mehmetoğlu İdris , Karaoğlan Hatice , Atalay Hüseyin , Solak Yalçın , Türk Süleyman 
Published: July 2010

AbstractIschemia‐Modified albumin (IMA) has been used as an early marker in the evaluation of the patients with acute coronary syndrome. We aimed to evaluate IMA in end‐stage renal disease (ESRD) patients on hemodialysis and the effect of albumin methods on albumin‐adjusted IMA levels. A total of 30 ESRD patients were included in this study. Serum IMA and albumin levels were measured before and after a hemodialysis session. Albumin concentrations were determined with bromocresol green and bromocresol purple methods. Postdialysis IMA and albumin‐adjusted IMA levels with two different albumin methods were significantly increased compared with the predialysis levels (P<0.05). However, we did not find any difference in albumin‐adjusted IMA levels in either at the beginning or at the end of the dialysis session. IMA levels increase after hemodialysis, whereas albumin method has no effect on albumin‐adjusted IMA levels. J. Clin. Lab. Anal. 24:273–277, 2010. © 2010 Wiley‐Liss, Inc.
Analytical performance evaluation of ADVIA Chemistry Carbamazepine_2 assay: minimal cross‐reactivity with carbamazepine 10, 11‐epoxide and none with hydroxyzine or cetirizine
Authors: Dasgupta Amitava , Reyes Meredith A. , Davis Barbara G. , Marlow Anne M. , Johnson Myrtle 
Published: July 2010

AbstractCarbamazepine is an anticonvulsant requiring routine therapeutic drug monitoring. Recently, Siemens Healthcare Diagnostic Division released a new carbamazepine assay: ADVIA Chemistry Carbamazepine_2 (Carbamazepine_2) for application on ADVIA analyzers. We evaluated the analytical performance of this assay as well as its potential cross‐reactivities with carbamazepine 10, 11‐epoxide, hydroxyzine, and cetirizine. The within‐run and between‐run precisions of the Carbamzepine‐2 assay were <6% and limit of detection was 0.5 µg/ml using ADVIA 1800 analyzer. The assay was linear up to a carbamazepine concentration of 20.0 µg/ml. The new method compared well with a widely used carbamazepine EMIT 2000 assay on the Hitachi 917 analyzer. Using 75 patients' specimens (where carbamazepine concentrations varied from 0.5 to 21.7 µg/ml) and carbamazepine EMIT 2000 as the reference method (x‐axis), we observed the following regression equation: y=1.04 x+0.32 (r=0.99). The new carbazepine_2 method was not affected by a hemoglobin concentration of 1,000 mg/dl, conjugated or unconjugated bilirubin concentration of 60 mg/dl, and triglyceride concentration of 1,000 mg/dl. In addition, this assay showed no cross‐reactivity with hydroxyzine or cetirizine and demonstrated minimal cross‐reactivity with carbamazepine 10, 11‐epoxide. We conclude that the ADVIA Chemistry carbamazepine_2 assay has adequate precision and accuracy for routine therapeutic drug monitoring of carbamazepine in clinical laboratories. J. Clin. Lab. Anal. 24:278–282, 2010. © 2010 Wiley‐Liss, Inc.
Adolescents' sense of community on myspace and facebook: a mixed‐methods approach
Authors: Reich Stephanie M. 
Published: July 2010

AbstractCommunities are foundational to the field of Community Psychology yet they are difficult to define and measure. Once viewed as social groups with ties to geographical locations, online communities interact free of physical or face‐to‐face contact. This cyberexistence makes the study of communities more challenging. Social networking sites (SNS), such as Facebook and MySpace, are referred to as online communities; however, research has yet to explore whether these sites engender a psychological sense of community (PSC) for users. This study reanalyzes focus group and survey data from high school and college students to investigate whether uses of SNS demonstrate key components of PSC (i.e., membership, influence, immersion, shared emotional connection, and an integration and fulfillment of needs). This mixed‐method analysis synthesizes data through a top‐down (confirming PSC categories) and bottom‐up (identifying emergent patterns/themes) analytic procedure. Results suggest that typical adolescent uses of SNS represent networked individualism, rather than online communities. © 2010 Wiley Periodicals, Inc.
Community life as a motive for migration from the urban center to the rural periphery in Israel
Authors: Arnon Sara , Shamai Shmuel 
Published: July 2010

AbstractA white house topped by a red roof, set in a garden, surrounded by a lawn dotted with trees and shrubs—this is not just a child's naive drawing. It is the aspiration of many in the modern world, Israelis among them. This case study deals with the inner migration of families, mainly from the urban center of Israel, to rural communities in its northern periphery. It is also an opportunity to examine counter‐urbanization characteristics and motivations, which contribute to our understanding of the role of community in this process. © 2010 Wiley Periodicals, Inc.
Latino family mental health: exploring the role of discrimination and familismo
Authors: Ayón Cecilia , Marsiglia Flavio F. , Bermudez‐Parsai Monica 
Published: July 2010

AbstractThe purpose of this study was to examine the role of discrimination andfamilismoon internalizing mental health symptoms among two generations of Latinos, youth and their parents, residing in the Southwest region of the United States. Data from the Latino Acculturation and Health Project was used to determine the direct and moderation effects of discrimination andfamilismoon internalizing mental health symptoms. The sample included 150 Latino youth–parent dyads who were immigrants or U.S. born. Descriptive results indicate that youth had significantly higher scores on the familismo scale whereas parents reported higher levels of perceived discrimination. Regression analyses results revealed direct effects of familismo and perceived discrimination on internalizing mental health symptoms. Implications for practice are discussed. © 2010 Wiley Periodicals, Inc.
Influence of perceived neighborhood safety on proactive and reactive aggression
Authors: Fite Paula J. , Vitulano Michael , Wynn Porche' , Wimsatt Amber , Gaertner Alden , Rathert Jamie 
Published: July 2010

AbstractThe current study examined associations between perceived neighborhood safety and proactive and reactive subtypes of aggression while also considering peer delinquency and poor parental monitoring in a community recruited sample of 89 children (56% male) ranging from 9 to 12 years of age (M=10.44,SD=1.14). In addition, neighborhood safety was examined as a moderator of the associations between peer delinquency and poor parental monitoring and proactive and reactive aggression. Findings suggested that while peer delinquency was positively associated with both proactive and reactive aggression, perceived neighborhood safety was only associated with proactive aggression. Moreover, neighborhood safety moderated the association between peer delinquency and proactive aggression, such that the association between peer delinquency and proactive aggression was stronger in neighborhoods perceived as less safe when compared neighborhoods perceived as more safe. Implications for findings are discussed. © 2010 Wiley Periodicals, Inc.
Adolescent mental health consumers' self‐stigma: associations with parents' and adolescents' illness perceptions and parental stigma
Authors: Moses Tally 
Published: July 2010

AbstractCurrently, little is known about adolescents' self‐stigma experiences as mental health (MH) treatment recipients. Hence, this study addresses the following two questions: (a) what are adolescents' and parents' perceptions of stigma and perceptions of the cause, controllability, and anticipated outcome (illness perceptions) of adolescents' MH problems? (b) to what extent do illness perceptions (adolescents and parents) and parents' own stigma experiences relate to adolescents' self‐stigmatization? The results, based on data from separate interviews with a voluntary sample of 60 adolescent–parent dyads, clients of MH wraparound services, suggest that approximately 20% of adolescents and parents reported significant concerns related to self‐stigmatization. Using multivariate analyses, we found that the three most prominent factors associated with adolescents' self‐stigma ratings included adolescents' perceptions of social skill deficits and trauma as causal factors pertaining to their mental health challenges, as well as parents' inclination to conceal their child's MH problems from others. © 2010 Wiley Periodicals, Inc.
Joven Noble: evaluation of a culturally focused youth development program
Authors: Tello Jerry , Cervantes Richard C. , Cordova David , Santos Susana M. 
Published: July 2010

AbstractThe purpose of this study was to describe and evaluate a promising program model, the Joven Nobel curriculum, for Latino male adolescents that promotes positive youth development and addresses prevention of a number of risk‐related sexual behaviors within a cultural context. A quasi‐experimental design was used that included a pre‐ and posttest assessment of 683 adolescent males. Results revealed that youth exposed to the 10‐week curriculum had significant increases in knowledge and decrease in behavior related to high risk sexual behaviors. Out of eight scale measures (ATSS, Perceived Risk, HIV Knowledge, CAPS: Talk, CAPS: Comfort Talk, CAPS: Cool Sex, ATA, Cultural Esteem, and Hombres Con Palabra) seven yielded a significant and positive outcome results. The curriculum appears effective in shaping youth's attitudes and beliefs about high‐risk sexual behaviors. © 2010 Wiley Periodicals, Inc.
Investigation of soluble microbial products in anaerobic wastewater treatment effluents
Authors: Wu Bingtao , Zhou Weili 
Published: July 2010

AbstractBACKGROUND: Anaerobic treatment of distillery wastewater, purified terephthalic acid (PTA) wastewater and synthetic glucose wastewater was conducted and the soluble microbial products (SMPs) in the anaerobic effluent were investigated.RESULTS: Gas chromatography‐mass spectrometry (GC‐MS) analysis showed that apart from the degradation residuals, the long chain alkanes, esters and acids totally accounted for the majority of the low molecular weight (MW) SMPs in the effluents. The sum of protein and polysaccharide SMPs in the effluent increased from 50 to 323 mg L−1 when organic loading rate (OLR) was increased from 2.5 to 10.5 kg m−3 d−1; when influent COD changed from 5000 to 10 000 mg L−1, the sum increased from 54 to 98 mg L−1 at about the same OLR of 5 kg m−3 d−1.CONCLUSION: The results showed that SMPs made up an important proportion of organic compounds in the anaerobic effluents; the main low MW SMPs were long chain alkanes, esters and acids. The protein and polysaccharide SMPs increased with the increasing OLR, while higher influent concentrations led to higher concentrations of SMPs at the same OLR. From the variation of protein and polysaccharide SMPs along the height of the anaerobic reactors, it could be inferred that the methanogens might have contributed more in SMP consumption. Copyright © 2010 Society of Chemical Industry
Right thoracic ectopic kidney in a child with breathing difficulties—Sonographic diagnosis
Authors: Maduekwe Echezona , Weinberg Brighita , Samaan Bassem , Sheferaw Ergeba , Suntharos Patcharapong 
Published: July 2010

AbstractCongenital intrathoracic ectopic kidney is a rare congenital abnormality that is usually found as an incidental lesion on chest radiographs. We report the case of a 6‐month‐old male with a 1‐day history of breathing difficulties whose chest radiograph revealed a soft tissue right basilar mass. Further investigation utilizing ultrasound revealed a thoracic kidney. This case demonstrates the importance of sonography as a diagnostic tool in identifying thoracic kidneys in the pediatric age group. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 39:108–110, 2011
Prenatal diagnosis and successful postnatal therapy of an atypical choroid plexus papilloma—Case report and review of literature
Authors: Hartge David Rafael , Axt‐Fliedner Roland , Weichert Jan 
Published: July 2010

AbstractThis report describes the perinatal management of a prenatally detected choroid plexus papilloma in an otherwise unaltered singleton pregnancy of a healthy woman. After elective Caesarean section, a successful embolization of the feeding vessel followed by a craniotomy and complete removal of the remaining tumor were performed. Histological examination confirmed the diagnosis. One‐, 2‐, and 3‐year follow‐up showed a normal development of the girl with a regular neuropediatric status. A review of literature including related papers listed in PubMed between 1985 and 2009 are included. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 38:377–383, 2010
One‐pot synthesis of spiro[diindeno[1,2‐b:2′,1′‐e]pyridine‐11,3′‐indoline]‐triones
Authors: Ghahremanzadeh Ramin , Fereshtehnejad Fatemeh , Bazgir Ayoob 
Published: July 2010

Abstract
magnified image A one‐pot and pseudo four‐component synthesis of spiro[diindeno[1,2‐b:2′,1′‐e]pyridine‐11,3′‐indoline]‐trione derivatives by cyclo‐condensation reaction of isatins, 1,3‐indandione, and ammonium acetate in refluxing acetic acid is reported. J. Heterocyclic Chem., (2010).