bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality and Agronomic Effects of Three High‐Molecular‐Weight Glutenin Subunit Transgenic Events in Winter Wheat
Authors: Graybosch Robert A. , Seabourn Bradford , Chen Yuanhong R. , Blechl Ann E. 
Published: November 2010

ABSTRACTQuality and agronomic effects of three transgenic high molecular weight glutenin subunit (HMW‐GS) events were characterized in advanced‐generation breeding lines of hard winter wheat (Triticum aestivum L.) in three Nebraska crop years. Two of the transgenic events studied, Dy10‐E and B52a‐6, overexpress HMW‐GS 1Dy10, while the third event, Dx5 +Dy10‐H, overexpresses HMW‐GS 1Dx5 and, to a much lesser extent, 1Dy10. In addition, novel proteins possessing solubility characteristics defined as HMW‐GS were present in Dx5+Dy10‐H and B52a‐6. Average grain yield of lines derived from the three transgenic events was statistically lower than that of a group of control cultivars and advanced breeding lines, but not lower than the mean values of respective nontransgenic siblings. Grain hardness was influenced by one of the events. Dx5+Dy10‐H produced harder kernels than controls, its nontransgenic siblings, and the two additional transgenic events. All three events produced doughs with unusual mixing properties, although not likely to be directly useful in commercial applications. As a consequence, loaf volumes were depressed to variable degrees by the three events. The results indicated that over‐expression of HMW‐GS could eventually lead to improved breadmaking quality by optimizing the level of overexpression or by development and characterization of additional events.
First Report of Botrytis hyacinthi on Pineapple Lily in California
Authors: Blomquist C. L. , Greene I. D. 
Published: November 2010

Pineapple lily (Eucomis vandermerwei) is grown for its exotic-looking flower spike that looks like a small pineapple. It is used as an accent plant in the garden or for its long-lasting cut flowers. In late November 2007, plant foliage was submitted to the California State Diagnostic Laboratory by a commercial grower for phytosanitary inspection before shipment of bulbs to Europe. Leaves had wet, brown, elliptical spots of 1 to 2 cm long, some with yellow halos. The interior of the older spots was pale brown and papery. Conidiophores typical of Botrytis spp. were observed on the abaxial side of the leaf in the interior of the older spots. Isolations on half-strength acidified potato dextrose agar (APDA) yielded a grayish mycelium with conidiophores developing on the plant tissue only. The agar was grayish yellow, especially when viewed from the underside. Conidiophores measured 275 to 650 μm × 15 to 20 μm (411.2 × 15.5 μm average). Conidia were light brown, subglobose to broadly ellipsoidal, and measuring 10 to 15 × 13 to 20 μm (11.8 × 16.2 μm average). Scattered, black sclerotia measuring 335 to 1,007 × 518 to 1,079 μm (668.9 × 743.9 μm average) formed on the medium after approximately 7 days. Pathogenicity was confirmed by inoculating four Eucomis spp. plants with one inoculation per leaf, four leaves per plant. Each leaf was wounded with a sterile pushpin and three agar plugs from 4-day-old cultures were placed in a plastic screw-cap lid filled with sterile water and clipped onto each wound. Plants were misted with water, covered with a plastic bag, and placed in a growth chamber at 16°C (12-h photoperiod) for 48 h after which the agar plugs and caps were removed. An equal number of plants were wounded and mock inoculated with APDA. Pathogenicity experiments were repeated. After 14 days, all inoculated leaves had lesions and the fungus was reisolated. No Botrytis spp. were isolated from the mock-inoculated plants. Our sequence of the intergenic spacer regions of one isolate, GenBank FJ10809, matched Botrytis hyacinthi sequence AJ716297 with 99.8% identity. Using molecular (3) and morphological characters (1,4), the pathogen was identified as B. hyacinthi, the cause of fire disease in hyacinths. This pathogen was described previously on hyacinths in Washington State (2), the Netherlands (4), and the United Kingdom (2). The importance and economic impact of this disease appears to be limited because it has only been observed on mature or senescing foliage and not bulbs. To our knowledge, this is the first report of B. hyacinthi causing a leaf spot on pineapple lily. References: (1) C. J. Gould et al. Plant Dis. Rep. 42:534, 1958. (2) W. C. Moore. Page 14 in: Diseases of Bulbs. Bull. No. 117. Minist. Agric. Fish. London, 1939. (3) M. Staats et al. Mol. Biol. Evol. 22:333, 2005. (4) J. Westerdijk and F. H. Van Beyma Thoe Kingma. Meded. Phytopathol. Lab. “Willie Commelin Scholten” 12:15, 1928.
Reproducibility of non‐standardised autonomic function testing in the pre‐operative assessment screening clinic*
Authors: Keet S. W. M. , Bulte C. S. E. , Boer C. , Bouwman R. A. 
Published: November 2010

SummaryBy convention, autonomic function tests are undertaken under standard test conditions that limit their implementation during routine pre‐operative assessment. We therefore evaluated the comparability of autonomic function tests under both non‐standardised and standardised test conditions in 20 healthy male subjects. Autonomic function was assessed using an ECG monitor and a continuous non‐invasive blood pressure measurement device. Under non‐standardised conditions, intraclass correlation for heart rate variability analysis was good for the low and high frequency bands (0.87; 95% CI 0.58–0.96 and 0.83; 95% CI 0.56–0.94, respectively), but moderate (0.65; 95% CI 0.14–0.86) for the very low frequency band; reproducibility was high for the expiration/inspiration ratio (0.89; 95% CI 0.71–0.96), Valsalva ratio (0.76; 95% CI 0.37–0.91) and handgrip test (0.76; 95% CI 0.35–0.91) (all p < 0.05) but was low for the response to quick standing. Reproducibility under standardised conditions was comparable to the above values. We demonstrated that reproducibility for most autonomic tests under non‐standardised conditions is acceptable and suggest that implementation of these tests during pre‐operative assessment may be feasible.
Intraplate subsidence and basin filling adjacent to an oceanic arc–continent collision: a case from the southern Caribbean‐South America plate margin
Authors: Bayona Germán , Montes Camilo , Cardona Agustín , Jaramillo Carlos , Ojeda Germán , Valencia Victor , Ayala‐Calvo Carolina 
Published: November 2010

ABSTRACTThe upper Campanian–Lower Eocene synorogenic sedimentary wedge of the Ranchería Basin was deposited in an intraplate basin resting on a tilted continental crustal block that was deformed by collision and subsequent subduction of the Caribbean Plate. Upper Cretaceous–Lower Eocene strata rest unconformably upon Jurassic igneous rocks of the Santa Marta Massif, with no major thrust faults separating the Santa Marta Massif from the Ranchería Basin. The upper Campanian–Lower Eocene succession includes, from base to top: foraminifera‐rich calcareous mudstone, mixed carbonate–siliciclastic strata and mudstone, coal and immature fluvial sandstone beds. Diachronous collision and eastward tilting of the plate margin (Santa Marta Massif and Central Cordillera) favoured the generation of accommodation space in a continuous intraplate basin (Ranchería, Cesar and western Maracaibo) during the Maastrichtian to Late Palaeocene. Terrigenous detritus from the distal colliding margin filled the western segments of the continuous intraplate basin (Ranchería and Cesar Basins); in the Late Paleocene, continental depositional systems migrated eastwards as far as the western Maracaibo Basin. In Early Eocene time, reactivation of former extensional structures fragmented the intraplate basin into the Ranchería‐Cesar Basins to the west, and the western Maracaibo Basin and Palmar High to the East. This scenario of continent–oceanic arc collision, crustal‐scale tilting, intraplate basin generation and fault reactivation may apply for Upper Cretaceous–Palaeogene syntectonic basins in western Colombia and Ecuador, and should be considered in other settings where arc–continent collision is followed by subduction.
Therapeutic failure in American cutaneous leishmaniasis is associated with gelatinase activity and cytokine expression
Authors: Maretti-Mira A C , de Oliveira-Neto M P , Da-Cruz A M , de Oliveira M P , Craft N , Pirmez C 
Published: November 2010

SummaryCutaneous lesions caused by Leishmania braziliensis infection occasionally heal spontaneously, but with antimonials therapy heal rapidly in approximately 3 weeks. However, about 15% of the cases require several courses of therapy. Matrix metalloproteinase-2 (MMP-2) and MMP-9 are gelatinases that have been implicated in other chronic cutaneous diseases and skin re-epithelialization. These enzymes are controlled by their natural inhibitors [tissue inhibitors of metalloproteinase (TIMPs)] and by some cytokines. Uncontrolled gelatinase activity may result in intense tissue degradation and, consequently, poorly healing wounds. The present study correlates gelatinase activity to therapeutic failure of cutaneous leishmaniasis (CL) lesions. Our results demonstrate an association between gelatinase activity and increased numbers of cells making interferon (IFN)-γ, interleukin (IL)-10 and transforming growth factor (TGF)-β in lesions from poor responders. Conversely, high levels of MMP-2 mRNA and enhanced MMP-2 : TIMP-2 ratios were associated with a satisfactory response to antimonials treatment. Additionally, high gelatinolytic activity was found in the wound beds, necrotic areas in the dermis and within some granulomatous infiltrates. These results indicate the importance of gelatinase activity in the skin lesions caused by CL. Thus, we hypothesize that the immune response profile may be responsible for the gelatinase activity pattern and may ultimately influence the persistence or cure of CL lesions.
Autoantibodies to GW bodies and other autoantigens in primary biliary cirrhosis
Authors: Stinton L M , Swain M , Myers R P , Shaheen A A , Fritzler M J 
Published: November 2010

SummaryAutoantibodies to intracellular targets in mitochondria and nuclei are serological hallmarks of primary biliary cirrhosis (PBC). One of the most recently identified cellular targets of PBC autoantibodies is a novel cytoplasmic structure referred to as GW bodies [GWB, G (glycine) W (tryptophan)-containing bodies (GWB)]. GWB are indentified as discrete cytoplasmic domains that are involved in mRNA processing via the RNA interference (RNAi) pathway. Key components of GWB include the proteins GW182, Ago2, RNA-associated protein 55 (RAP55) and Ge-1/Hedls. The primary objective was to study the frequency and clinical association of antibodies directed to GWB components, in 109 PBC patients. Autoantibodies to mitochondrial antigen–pyruvate dehydrogenase complex (M2), branched-chain 2-oxo-acid dehydrogenase complex and 2-oxo glutarate dehydrogenase complex (3E-BPO), gp210, sp100, promyelocytic leukaemia cell antigen (PML) and liver kidney microsomal-1 antigen (LKM-1) were detected by a line immunoassay and antibodies to GWB (GW182, RAP55, Ge-1, GW2, GW3) and glutamate receptor interacting protein (GRIP)-associated protein-1 (GRASP-1), by an addressable laser bead immunoassay (ALBIA). The most common GWB autoantigen targets were: RAP55-28%, GW182-12%, GW2-2% and antibodies to GRASP-1-17%. By comparison, the frequency of reactivity to established PBC autoantigens was: gp210, 27%; sp100, 27% and PML, 17%. None of the autoantibodies were associated with differences in Mayo risk score or liver decompensation. This study is the first study to show that antibodies to RAP55, GW182 and GRASP-1 are the most common GWB targets in PBC.
Toll-like receptor (TLR)-4 mediates anti-β2GPI/β2GPI-induced tissue factor expression in THP-1 cells
Authors: Zhou H , Yan Y , Xu G , Zhou B , Wen H , Guo D , Zhou F , Wang H 
Published: November 2010

Summary
Our previous study demonstrated that annexin A2 (ANX2) on cell surface could function as a mediator and stimulate tissue factor (TF) expression of monocytes by anti-β2-glycoprotein I/β2-glycoprotein I complex (anti-β2GPI/β2GPI). However, ANX2 is not a transmembrane protein and lacks the intracellular signal transduction pathway. Growing evidence suggests that Toll-like receptor 4 (TLR-4) might act as an ‘adaptor’ for intracellular signal transduction in anti-β2GPI/β2GPI-induced TF expressing cells. In the current study, we investigated the roles of TLR-4 and its related molecules, myeloid differentiation protein 2 (MD-2) and myeloid differentiation factor 88 (MyD88), in anti-β2GPI/β2GPI-induced TF expressing human monocytic-derived THP-1 (human acute monocytic leukaemia) cells. The relationship of TLR-4 and ANX2 in this process was also explored. Along with TF, expression of TLR-4, MD-2 and MyD88 in THP-1 cells increased significantly when treated by anti-β2GPI (10 µg/ml)/β2GPI (100 µg/ml) complex. The addition of paclitaxel, which competes with the MD-2 ligand, could inhibit the effects of anti-β2GPI/β2GPI on TLR-4, MD-2, MyD88 and TF expression. Both ANX2 and TLR-4 in THP-1 cell lysates could bind to β2GPI that had been conjugated to a column (β2GPI-Affi-Gel). Furthermore, TLR-4, MD-2, MyD88 and TF expression was remarkably diminished in THP-1 cells infected with ANX2-specific RNA interference (RNAi) lentivirus (LV-RNAi-ANX2), in spite of treatment with a similar concentration of anti-β2GPI/β2GPI complex. These results indicate that TLR-4 and its signal transduction pathway contribute to anti-β2GPI/β2GPI-induced TF expression in THP-1 cells, and the effects of TLR-4 with ANX2 are tightly co-operative.
Prevention of excessive collagen accumulation by human intravenous immunoglobulin treatment in a murine model of bleomycin-induced scleroderma
Authors: Kajii M , Suzuki C , Kashihara J , Kobayashi F , Kubo Y , Miyamoto H , Yuuki T , Yamamoto T , Nakae T 
Published: November 2010

Summary
Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrotic changes in skin and other organs involving excessive collagen deposition. Here we investigated the effect of intravenous immunoglobulin (IVIG) on fibrosis in a murine model of bleomycin (BLM)-induced scleroderma. Scleroderma was induced in C3H/He J mice by subcutaneous BLM injections daily for 35 days. The collagen content in skin samples from the BLM-injected group (6·30 ± 0·11 mg/g tissue) was significantly higher than the PBS group (5·80 ± 0·10 mg/g tissue), and corresponded with dermal thickening at the injection site. In contrast, mice treated with IVIG for 5 consecutive days after initiating BLM injection showed lesser collagen content significantly (IVIG group, 5·61 ± 0·09 mg/g tissue; BLM vs. IVIG). In order to investigate the cellular and protein characteristics in the early stage of the model, the skin samples were obtained 7 days after the onset of experiment. Macrophage infiltration to the dermis, monocyte chemoattractant protein (MCP-1)-positive cells, and increased TGF-β1 mRNA expression were also observed in the BLM group. IVIG inhibited these early fibrogenic changes; MCP-1 expression was significantly lesser for the IVIG group (1·52 ± 0·19 pg/mg tissue) than for the BLM group (2·49 ± 0·26 pg/mg tissue). In contrast, TGF-β1 mRNA expression was significantly inhibited by IVIG. These results suggest that IVIG treatment may inhibit macrophage recruitment to fibrotic sites by down regulating MCP-1 and TGF-β production, and thus could be a potential drug for managing fibrotic disorders such as SSc.
Using plant functional traits to understand the landscape distribution of multiple ecosystem services
Authors: Lavorel Sandra , Grigulis Karl , Lamarque Pénélope , Colace Marie‐Pascale , Garden Denys , Girel Jacky , Pellet Gilles , Douzet Rolland 
Published: November 2010

Summary 1. Spatially explicit understanding of the delivery of multiple ecosystem services (ES) from global to local scales is currently limited. New studies analysing the simultaneous provision of multiple services at landscape scale should aid the understanding of multiple ES delivery and trade‐offs to support policy, management and land planning. 2. Here, we propose a new approach for the analysis, mapping and understanding of multiple ES delivery in landscapes. Spatially explicit single ES models based on plant traits and abiotic characteristics are combined to identify ‘hot’ and ‘cold’ spots of multiple ES delivery, and the land use and biotic determinants of such distributions. We demonstrate the value of this trait‐based approach as compared to a pure land‐use approach for a pastoral landscape from the central French Alps, and highlight how it improves understanding of ecological constraints to, and opportunities for, the delivery of multiple services. 3. Vegetative height and leaf traits such as leaf dry matter content were response traits strongly influenced by land use and abiotic environment, with follow‐on effects on several ecosystem properties, and could therefore be used as functional markers of ES. 4. Patterns of association among ES were related to the dominant traits underlying different ecosystem properties. The functional decoupling between height and leaf traits provided alternative pathways for high agronomic value, as well as determining hot and cold spots of ES. Traditional land uses such as organic fertilization and mowing or altitude summer grazing were also linked with ES hot spots, because functional characteristics supporting fodder production and quality are compatible with species and functional diversity. 5. Synthesis. Analyses of ES using plant functional variation across landscapes are a powerful approach to understanding the fundamental ecological mechanisms underlying ES provision, and trade‐offs or synergies among services. Sustainable management of species and functionally diverse grassland could simultaneously aim at conserving biodiversity and locally important ES by taking advantage of correlations and trade‐offs among different plant functional traits.
Purple loosestrife suppresses plant species colonization far more than broad‐leaved cattail: experimental evidence with plant community implications
Authors: Hovick Stephen M. , Bunker Daniel E. , Peterson Chris J. , Carson Walter P. 
Published: November 2010

Summary 1. Dominant plant species, whether native or invasive, often change community composition and cause decreases in diversity. Still invasive species are considered more deleterious to communities than dominant natives, although evidence for this is surprisingly rare. We tested two hypotheses: (i) an exotic invasive species will have greater impacts at the community level than a dominant native and (ii) this deleterious impact will be exacerbated with eutrophication. 2. Both hypotheses were tested by evaluating colonizer success in large, well‐replicated experimental monocultures of two dominant and widespread wetland species, invasive purple loosestrife (Lythrum salicaria) and native broad‐leaved cattail (Typha latifolia). To facilitate comparisons, we standardized competitive effects by canopy biomass and by light availability beneath the canopy. The latter is a novel approach that accounts directly for resource reductions caused by community dominants. 3. Loosestrife was particularly detrimental to rare species and dramatically reduced colonizer success compared to cattail by nearly all of our metrics, including colonizer biomass (50.2% lower), species richness (34.2% lower), Shannon diversity (35.8% lower) and the proportion of mesocosms that were colonized (38.5% lower). Moreover, 15 of 16 uncommon species failed to colonize loosestrife communities. Graminoids fared poorly in loosestrife monocultures, but forb biomass (predominantly Sagittaria latifolia) was 3.5 times higher there. These results suggest that over time, plant communities under loosestrife canopies will contrast sharply with those under cattail. Contrary to our second hypothesis, fertilization did not exacerbate loosestrife’s ability to suppress colonizers, relative to that of cattail. 4. Canopy biomass and light attenuation were similar for cattail and loosestrife, yet biomass explained little variation in colonizer success. Increasing light availability in the understorey increased colonizer richness and diversity only under cattail canopies, suggesting loosestrife suppresses colonization via below‐ground competition while cattail does so via light reduction. 5. Synthesis. Ours is the first study to show that an invasive species suppresses colonizers much more than a dominant native and to identify contrasting mechanisms by which this may occur. Biomass‐based comparisons of competitive effects may have limited utility for highly productive dominant species generally, thus our approach also offers a viable new alternative that could be applied broadly.
Gene network architecture as a canvas for the interpretation of ecological genomics investigations
Authors: LANDRY CHRISTIAN R. , AUBIN‐HORTH NADIA 
Published: November 2010

New technologies promise to revolutionize the field of molecular ecology. This technological progress comes with its own set of challenges. Among the most important ones is the analysis and interpretation of the data in a way that tells us about the molecular causes of the phenotype of interest and its consequences. In this issue, Whitehead et al. (2010) reveal part of the mechanistic basis of evolved pollution tolerance by studying the developmental and transcriptional response of tolerant and sensitive fish embryos to polychlorinated biphenyls (PCBs), a pollutant commonly found in coastal waters of the United States. By integrating their gene expression profiling data with phenotypic data on individuals along with what is known about pathways by which this pollutant acts in zebrafish and mammals, they are able to suggest detailed mechanisms that have evolved to allow a fish population to adapt to a very damaging pollutant and develop normally.
Frozen F1’s amidst a masterpiece of nature: new insights into the rare hybrid origin of gynogenesis in the Amazon molly (Poecilia formosa)
Authors: ROGERS S. M. , VAMOSI S. M. 
Published: November 2010

AbstractAll‐female ‘species’ of fish have been shown to be great models in ecological and evolutionary studies because of the insights they can provide into the origin and evolution of asexuality, the ecology of hybrids, associations between genotype and environment, and the maintenance of sex. Gynogenetic organisms that evolved from sexual ancestors, and combine the disadvantageous traits from sexuality and asexuality, have long baffled evolutionary biologists trying to understand their origin and persistence with their sympatric sexual counterparts. In this issue, a new study using an integrated molecular phylogenetic and classical genetic approach has uncovered compelling evidence regarding the obscure asexual origin of the Amazon molly, Poecilia formosa. By performing an extensive phylogeographic analysis, Stöck et al. (2010) provide evidence that the Amazon molly arose only once within its history, with monophyly being strongly supported by mitochondrial DNA and microsatellite analyses. This result, combined with an elaborate failed attempt to resynthesize the lineage, suggests that vertebrate gynogens such as the Amazon molly are not rare because they are at a disadvantage to their sexual counterparts, but because the genomic conditions under which they arise are rare. Organisms that apparently combine the disadvantages of both sexuality and asexuality remain difficult to understand from both an ecological and an evolutionary perspective, and Stöck et al. (2010) highlight several outstanding important questions. Nonetheless, given that we now have a better knowledge of the origin and history of this unique ‘species’, this should allow researchers to better understand how these frozen F1’s can persist amidst the masterpiece of nature.