bluuebunny/crossref_metadata_2025_split huggingface dataset:
Male infertility due to idiopathic disorders of spermatogenesis: no association with HLA
Authors: Mueller‐eckhardt G. , Krause W. , Schierke G. , Mueller‐eckhardt C. 
Published: July 2010

HLA‐ABC and DR antigens were determined in 65 male patients suffering from infertility due to idiopathic disorders of spermatogenesis, and in one family including 3 brothers with azoospermia.No statistically significant deviations of antigen frequencies were observed in the patient group when compared with 929 (ABC) or 206 (DR) healthy control individuals. From the family data, a segregation of an hypothetical disease susceptibility gene with HLA could not be deduced.
Soluble HLA antigens not detected in human immunoglobulin preparations
Authors: MCLEAN I. L , FAED J. M , MILLIGAN L. M , ADAMS D. D 
Published: July 2010

Immunoglobulin concentrates prepared from subjects of known HLA specificity were assayed for the presence of soluble HLA antigens by the inhibition of cytotoxic HLA alloantisera using a microabsorption procedure.The immunoglobulin preparations inhibited alloantisera of all specificities tested, regardless of the HLA type of the immunoglobulin donor, indicating that the observed inhibition was non‐specific. Addition of extra complement reduced the inhibitory effects of the immunoglobulin concentrates.It is concluded that the inhibition of HLA alloantisera demonstrated by this technique is due to the anticomplementary activity of the immunoglobulin preparations, rather than their content of specific soluble HLA antigens.
Structural and genetic analyses of HLA class I molecules using monoclonal xenoantibodies
Authors: REBAÏ NAJET , MALISSEN BERNARD 
Published: July 2010

The monoclonal antibodies B1.23.2 and B9.12 were developed to characterize human class I major histocompatibility gene products. They detect two different epitopes and define on a given lymphoblastoid B cell line two subsets of β2micro‐globulin‐complexed HLA class I molecules. One subset reacts with B9.12 and B1.23.2 and the other one expresses only the B9.12 epitope. Binding assays were performed on C3H mouse L cells which had been transformed with various single HLA‐class I genes and the two detected molecular subsets were shown to be encoded by different genetic loci.Unlike B1.23.2, B9.12 detects all the β2m‐complexed molecules expressed in human B cell lines and recognizes an epitope different from the one defined by W6/32. In contrast to the vast majority of the other reported anti‐class I monoclonal antibodies (including B9.12), B1.23.2 recognizes an epitope expressed on both β2m‐associated and ‐free HLA class I heavy chains.
HLA‐DB3: population distribution and family studies of a new HLA‐D antigen associated with HLA‐DR4 in Caucasoids
Authors: Jaraquemada D. , Ollier W. , Okoye R. C. , Sachs J. A. , Festenstein H. , Grosse‐Wilde H. 
Published: July 2010

On the basis of studies with 6 HTCs in four different families the new cluster DB3 identified in the 8th Workshop can be considered an established specificity of the HLA‐D series. In 112 healthy Caucasoids resident in South East England the frequency of this antigen was 2.6% similar to the frequency found in 142 Caucasoid patients with rheumatoid arthritis. The frequency in 54 unrelated Chinese individuals from Shanghai and of 120 Nigerians was 7.5% and 3%, respectively.All Caucasoid individuals who were DB3 were also DR4 whereas no such association was found in the two other population groups.
Study of alleles of the second complement component (C2) on Canadian HLA haplotypes
Authors: MARSHALL W. H. , BARNARD J. M. , CHURCHILL D. , FARID N. R. , GRANDY R. , LARSEN B. , PAYNE R. H. , SKANES V. M. , EBERS G. C. , PATY D. W. , MERVART H. , SCHROEDER M. L. 
Published: July 2010

Typing for genetic variation in the second complement component C2 was performed on sera from HLA haplotyped Canadian families. Part of the data has been studied and analysed as a population; in addition there is a further random collection of haplotypes bearing the C2*2 allele. In the population data there were 444 separate haplotypes from unrelated parents or other founders: 4.7% of the haplotypes carried the uncommon allele C2*2; one haplotype carried the rare C2*3. Study of C2 2–1 heterozygotes in the population data revealed 59 haplotypes which carried the common C2*1 allele and one which carried a deficiency allele C2*0. The remaining haplotypes carried either C2* 1 or else an undetectable C2*0 allele. In the entire data there were 281 meioses informative for C2. The only recombinant between HLA‐B and C2 showed the C2 locus to be on the DR side of the B locus. Strong allelic association between C2 *2 and Bw22 and less strong association between C2 *2 and B15 suggested the possibility of two ancestral C2 mutants. Examination of other markers on these and subsequently collected haplotypes do not conflict with this idea since the B15 haplotypes mostly carry C4A *4, C4B*2 whilst the Bw22 haplotypes mostly carry C4A*4, C4B*4. The alternative idea, that there was one original mutant which crossed over from a B15 to a Bw22 haplotype or vice versa is not excluded, however. Since approximate equilibrium has been reached between Bw22 and the HLA‐A locus alleles on these C2*2 bearing haplotypes, we conclude that this mutation is at least 5000 years old.Other haplotypes carrying C2*2 are assumed to be ancestral recombinants; if this is true, the C2 locus map position between HLA‐B and HLA‐DR is confirmed. Study of C2 mutation may provide a model for understanding the genetics of some disease susceptibility genes in the HLA region.
Paradoxical inheritance of HLA‐Iinked susceptibility to rheumatoid arthritis
Authors: De Vries R. R. P. , Nijenhuis L. E. , Khan M. A. , Mehra N. K. 
Published: July 2010

In order to clarify the discrepancy between population studies showing association of rheumatoid arthritis (RA) with HLA‐DR4/‐Dw4 and family studies failing to show linkage with HLA, we analysed 16 multicase families in which RA and DR4 status of both parents was known. 120 HLA‐haplotypes of affected and unaffected children could be analysed for co‐segregation with RA. In a combined analysis of both affected and unaffected children co‐segregation of RA with the DR4 carrying haplotype was observed when both parents were unaffected (p=0.001). Co‐segregation of RA with one of the two haplotypes of affected parents was observed (p=0.01), but in this case there was no preference for the DR4 carrying haplotype. In both cases preferential inheritance of the other (not associated with RA) haplotype was observed in unaffected siblings.These data indicate that susceptibility to RA is controlled by an HLA‐linked gene. This gene is often but not always identical to the gene coding for a product carrying the DR4 epitope or in strong linkage disequilibrium with it. Combined with previous population data, the present data provide evidence for genetic heterogeneity of RA. Finally, they contain a paradox, based on which a new hypothesis for HLA‐linked susceptibility to RA is formulated.
An HLA‐DR/DP recombinant family involving DPw6. Evidence for cross‐reactivity between DPw6 and GNN2B
Authors: ØDum Niels , Hartzman Robert , Jakobsen Bodil K. , Morling Niels , Platz Per , Robbins Fu‐Meei , Ryder Lars P. , Shaw Steven , Svejgaard Arne 
Published: July 2010

HLA typing of an HLA‐B/GLO recombinant family using bulk‐expanded DP(SB) alloreactive T‐cells (GNN1 to 6) as well as conventional HLA‐ABC, D, DR. and DQ typing showed that a paternal recombination had taken place between HLA‐DR and DQ on the one hand and HLA‐DP(SB) on the other. The recombinant child was DPw4, 6‐heterozygous and differed in terms of class II determinants only for DPw6 from two otherwise HLA identical siblings, who were probably DPw4/4‐homozygous. These siblings did not stimulate the recombinant in MLC whereas they responded to his cells indicating that DPw6 can stimulate in primary MLC. Moreover, it was possible to generate and bulk‐expand DPw6‐reactive lymphocytes (PLs) between MT and MA as responders and BN as stimulator. The correlation coefficient (r) between the reaction of these PLs and the GNN6 (anti‐DPw6) reagents was 1.0 when tested against a panel of 71 individuals.This is the first report demonstrating that the DPw6 gene, like the DPw1‐w5 genes, is located between DR/DQ and GLO.The frequency of the DPw6 antigen in 41 unrelated, randomly selected Danes was 7%. HLA‐DP typing of the BN family revealed that the DPw6 positive but not DPw6 negative family members gave intermediate responses with one (GNN2B) but not with another (GNN2A) anti‐DPw2‐reagent. Studies of 63 healthy individuals (unrelated to the BN family) revealed seven similar discrepancies between GNN2A and GNN2B. Strikingly, all four DPw6 positive stimulators gave rise to such discrepancies indicating that the GNN2B reagent is cross‐reactive and recognize a common part of the DPw2 and DPw6 molecules. No cases of GNN2A+/GNN2B‐ stimulators were found.
Gamma ray‐induced mutants as a tool for the production and characterisation of monoclonal antibodies against HLA‐alloantigens
Authors: Spring Bärbel , Pawelec Graham , Ziegler Andreas 
Published: July 2010

To simplify the screening procedure for murine monoclonal antibodies specific for polymorphic HLA determinants, spleen cells from a mouse immunized with the human cell line BJAB‐B95.8.6 were fused with NSI mouse myeloma cells, and hybridoma supernatants were screened for their reactivity on BJAB‐B95.8.6 and two gamma ray‐induced HLA‐loss mutants of this line.The use of these HLA‐loss mutants allowed the rapid identification of two new al‐lospecific MOABs designated TÜ160 and TÜ161. Serological as well as biochemical studies revealed TÜ160 to be specific for HLA‐A2, and TÜ161 for HLA‐B13 molecules, respectively. Both MOABs were determined to be antibodies of the IgG class and were able to precipitate their antigens from lysates of radioactively labeled cells.
HLA‐DPw antigens in Danes and Eskimos as determined by local bulk PLT‐reagents
Authors: Petersen C. Munck , Mösller B. K. , Lamm L. U. 
Published: July 2010

Primed Lymphocyte Typing cells (PLT‐cells) with specificities for HLA‐DP w1‐w6 were generated. Responder and stimulator lymphocytes were from highly selected donors. Responder cells were primed twice (for 10 and 6 days respectively) prior to a third priming and bulk expansion in IL‐2 containing medium.The coefficient of correlation between typing results obtained with local PLT‐reagents and results based on 9th International Histocompatibility Workshop reagents was 0.91. As determined by batch to batch and day to day variations (different batch on different day), the reproducibility of +/‐ scorings and assignments obtained with local reagents was 0.87 (range 0.64–1.0) and 0.9 (range 0.69–1.0) respectively.The HLA‐DPw gene frequencies in a population of 88 unrelated Danes and a population of 48 unrelated Greenland Eskimos were determined. The DPw gene frequencies observed in Danes were very similar to those reported in other Caucasian populations. Compared to Caucasians, a significantly lower frequency of DPw3 and a somewhat higher frequency of DPw2 were found in Eskimos. The findings did not differ from the Hardy‐Weinberg expectations. No linkage disequilibrium between DR and DP was observed in the Eskimo population, whereas the DR3 ‐ DPw1 association in Danes (Caucasians) was confirmed.With regard to HLA‐DR, DR1, DR4 and DR7 were found to be more frequent in Eskimos than in Danes (not significant using corrected p values).The genetic distance between the Danish and the Eskimo population was 0.21 as determined by DPw and 0.24 as determined by DR gene frequencies.
IL‐12Bpro and GSTP1 polymorphisms in association with silicosis
Authors: Stanilova S. , Miteva L. , Prakova G. 
Published: July 2010

AbstractTo assess the role of IL‐12Bpro and GSTP1 polymorphisms on induced interleukin (IL)‐12p40 production in respect to silicosis development, we examined their distribution in 63 silicosis patients and 165 healthy donors. Decreased frequencies of IL‐12Bpro‐2 allele (42% vs 62.5%, P = 0.0042) and genotype 22 (19% vs 40%, P = 0.02) in patients compared with exposed healthy donors suggested their protective role in silicosis susceptibility. The GSTP1 A/G polymorphism was also associated with silicosis susceptibility. The allele G and genotype GG were overrepresented among patients than among healthy men (36% vs 23%, P = 0.013; 14% vs 2%, Pc = 0.012). Also, patients’ peripheral blood mononuclear cell (PBMC) produced higher IL‐12p40 than healthy donors’ depending on both IL‐12Bpro and GSTP1 polymorphisms. In conclusion, homozygosity of high producer IL‐12p40 genotype IL‐12Bpro‐11 and GSTP1‐GG had the highest genetic risk of silicosis development in Bulgarian miners.
Influenza A (H1N1) virus‐induced hepatocellular injury in a kidney transplant patient
Authors: Daudé M. , Mansuy J.M. , Guitard J. , Basse G. , Esposito L. , Izopet J. , Rostaing L. , Kamar N. 
Published: July 2010

M. Daudé, J.M. Mansuy, J. Guitard, G. Basse, L. Esposito, J. Izopet, L. Rostaing, N. Kamar. Influenza A (H1N1) virus‐induced hepatocellular injury in a kidney transplant patient. Transpl Infect Dis 2011: 13: 70–72. All rights reserved.Abstract: The swine‐origin influenza A (H1N1) virus is mainly responsible for flu. No hepatitis attributable to H1N1 virus has been previously documented. Herein, we report on a kidney transplant patient who developed influenza H1N1 virus‐induced hepatocellular injury. The patient's body temperature was only somewhat elevated, and pulmonary and flu symptoms were mild. H1N1 virus was detected by polymerase chain reaction assay in nasopharyngeal and bronchoalveolar swabs, as well as in the serum. The hepatocellular injury episode resolved after the patient had been placed on oseltamivir therapy. This observation suggests that acute hepatocellular injury could be linked to the influenza H1N1 virus.
Variations in oil palm (Elaeis guineensis Jacq.) progeny response to high aluminium concentrations in solution culture
Authors: Cristancho R. J. A. , Hanafi M. M. , Syed Omar S. R. , Rafii M. Y. 
Published: July 2010

AbstractAluminium (Al) phytotoxicity is an important soil constraint that limits crop yield. The objectives of this study were to investigate how growth, physiology, nutrient content and organic acid concentration is affected by Al, and to assess the degree of Al tolerance in different oil palm progeny (OPP). Four OPPs [‘A’ (Angola dura × Angola dura), ‘B’ (Nigerian dura × Nigerian dura), ‘C’ (Deli dura × AVROS pisifera) and ‘D’ (Deli dura × Dumpy AVROS pisifera)] were grown in different Al concentrations (0, 100 and 200 μm) in aerated Hoagland solution, pH 4.4, for 80 days. We observed a severe reduction (57.5%) in shoot dry weight, and root tips were reduced by 46.5% in 200 μm Al. In ‘B’ and ‘C’, the majority of macro‐ and micronutrients in plants were reduced significantly by 200 μm Al, with Mg being lowered by more than 50% in roots and shoots. The 200 μm Al treatment resulted in a 56.50% reduction in total leaf area, a 20% reduction in net photosynthesis and a 17% reduction in SPAD chlorophyll value in the third leaf. Root tips (0–5 mm) showed a significant increase in oxalic acid content with increasing Al concentration (∼5.86‐fold); progeny ‘A’ had the highest concentration of oxalic acid. There was a significant interaction between Al concentration × OPP on total leaf number, root volume, lateral root length, Mg and K in root and shoot tissues, and Ca and N in shoots. The OPPs could be ranked in their tolerance to Al as: ‘A’ > ‘D’ > ‘B’ > ‘C’.
Genetic and physiological analysis of biennialism in Hyoscyamus niger
Authors: Schläppi M. 
Published: July 2010

AbstractA genetic and physiological study of biennialism in the diploid selfer Hyoscyamus niger (black henbane), an obligate long‐day plant, is described. Three annual and two biennial accessions that were homozygous for their respective growth habits were selected. The early‐flowering trait of two annual accessions was dominant over the late‐flowering trait of the third annual accession. The late‐flowering annual accession, but not the early‐flowering ones, responded to vernalization. Two biennial accessions remained vegetative after more than 1 year in soil and thus had an obligate vernalization requirement. Crosses between annual and biennial accessions showed that biennialism was conferred through a single dominant gene. However, plants containing only one copy of this dominant gene were transformed from biennials into very late‐flowering winter‐annual plants that responded more rapidly to vernalization than biennials. Taken together, these results indicated that there were allelic differences in photoperiod‐specific flowering time genes and that biennialism was a dose‐dependent trait with incomplete dominance. Models for flowering time regulation in henbane involving photoperiod‐, vernalization‐, and most likely gibberellin‐specific pathways are discussed.
Cerebral phaeohyphomycosis caused by an Exophiala. species
Authors: Tintelnot Kathrin , de Hoog G. S. , Thomas E. , Steudel W. I. , Huebner K. , Seeliger H. P. R. 
Published: July 2010

Summary. A 45‐year‐old woman was found to have cerebral phaeohyphomycosis caused by an Exophiala species. The portal of entry of the fungus remained unknown. Despite surgery and antimy‐cotic therapy the patient died from circulation failure. The fungus is compared to several related Exophiala species.Zusammenfassung. Eine 45jährige Frau er‐krankte an einer zerebalen Phaeohyphomykose, hervorgerufen durch eine Exophiala‐Art. Die Ein‐trittspforte dieses Schwärzepilzes wurde nicht ge‐funden. Trotz chirurgischer und antimykotischer Therapie starb die Patientin an Komplikationen des großen Kreislaufs. Der Stamm wird mit einigen ver‐wandten Exophiala‐Arten verglichen.
Specific digestive deficiency of phagocytes in paracoccidioidomycosis. Its absence in peripheral blood neutrophils of members of the nuclear family of patients. An initial report
Authors: Urquiola G. , Goihman‐Yahr M. , de Albornoz M. C. Bastardo , Istúriz G. , Viloria N. , Saavedra N. , Carrasquero M. , Pereira J. , de Gómez M. H. , Román A. , Martín B. San 
Published: July 2010

Summary. Paracoccidioidomycosis (PARA) affects only a minority of individuals, who have presumably been exposed to the causative fungus (Paracoccidioides brasiliensis). Neutrophils (PMNs) from patients with PARA show a significant and specific digestive deficiency phagocytosed P. brasiliensis in vitro. It is not known whether the defect is acquired after contact with the fungus, or precedes it. We studied the spouses and offspring of three patients with PARA. Individuals studied stayed in the same house as their husband or father. None of the relatives had evidence of PARA, and their PMNs showed no defect in their ability to digest or kill the fungus. Relatives showed no indication of sensitization against P. brasiliensis. These results are compatible with the view that effective contact with P. brasiliensis may occur only under restricted conditions and that the defect in digestive ability is an infrequent and specific occurrence.Zusammenfassung. An Paracoccidioidomykose erkrankt vermutlich nur eine Minderheit aller Individuen, die dem Erreger Paracoccidioides brasiliensis exponiert sind. Die neutrophilen Granulozyten (PMN) der Patienten mit Paracoccidioidomykose zeigen eine signifikante Abbauhemmung fir phagozytierte P. brasiliensis in vitro. Es ist nicht bekannt, ob dieser Defekt erst nach dem Erregerkontakt erworben wird, oder ob er schon vorher bestand. Wir haben Ehegatten und Nachkommen von drei Paracoccidioidomykose‐Patienten untersucht. Die Probanden lebten gemeinsam in einer Wohnung mit den Patienten. Bei keinem der verwandten Probanden bestand irgendein Hinweis auf eine Paracoccidioidomykose, und ihre PMN zeigten keine Hemmung im intrazellu‐laren Abbau und der Abtötung des Pilzes. Bei den Probanden wurde auch keine Sensibilisierung gegen P. brasiliensis aufgedeckt. Diese Ergebnisse sind vereinbar mit der Ansicht, daß ein sensibili‐sierender Kontakt mit P. brasiliensis nur unter eingeschränkten Bedingungen statthat und daß der Defekt in der intrazellulären Abbaufahigkeit ein seltenes und spezifisches Ereignis ist.
Anxiety, neuroticism and oxidative stress: Cross‐sectional study in non‐smoking college students
Authors: Matsushita Masateru , Kumano‐Go Takayuki , Suganuma Nakamori , Adachi Hiroyoshi , Yamamura Schuhei , Morishima Hiroko , Shigedo Yoshihisa , Mikami Akira , Takeda Masatoshi , Sugita Yoshiro 
Published: July 2010

Aims:  Oxidative stress has gained attention recently in psychiatric medicine and has been reported to be associated with various diseases. However, the psychosocial factors involved in oxidative stress are still not well understood. Thus, the aim of this study was to examine whether anxiety levels and neuroticism were associated with serum oxidative and anti‐oxidative status in healthy college students.Methods:  Participants in this cross‐sectional study were 54 non‐smoking college students. Their serum oxidative status was determined by reactive oxygen metabolites (ROM) and the biological anti‐oxidant potential. Anxiety levels and neuroticism were assessed using the State–Trait Anxiety Inventory and the NEO Five‐Factor Inventory, respectively.Results:  Correlation analysis showed an association of increased ROM concentration with elevated anxiety levels (State, ρ = 0.39, P = 0.046; Trait, ρ = 0.44, P = 0.024) and the personality trait of neuroticism (ρ = 0.47, P = 0.016) in female students. However, the ROM concentration in male students was not associated with the anxiety level or any personality trait.Conclusion:  Although these findings suggest that neurotic and anxious female students tend to be exposed to oxidative stress, these linkages should be confirmed by multivariate analysis in future research.
Treatment outcomes of sorafenib for first line or cytokinerefractory advanced renal cell carcinoma in Japanese patients
Authors: Ueda Takeshi , Imamura Yusuke , Komaru Atsushi , Fukasawa Satoshi , Sazuka Tomokazu , Suyama Takahito , Naya Yukio , Nihei Naoki , Ichikawa Tomohiko , Maruoka Masayuki 
Published: July 2010

AbstractThe objective of the present study was to document the treatment efficacy and safety of sorafenib in Japanese patients with advanced renal cell carcinoma (RCC). A retrospective analysis of 50 consecutive patients with metastatic RCC between January 2005 and December 2009 was carried out. Patients received sorafenib after failed cytokine therapy or first‐line sorafenib treatment. All received 400 mg of sorafenib orally twice daily. Five of 14 patients with bone metastases were also given bisphosphonates. Tumor response was evaluated every 1–2 months according to the Response Evaluation Criteria in Solid Tumors. Adverse events (AE) were evaluated at each visit during and after treatment, and were recorded according to the National Cancer Institute's Common Terminology Criteria for Adverse Events version 3.0. Dose modification of sorafenib was permitted if grade 3 or 4 AE occurred. Treatment continued until disease progression or treatment intolerance occurred. Partial response, and stable disease as best objective responses were observed in 11 (22%) and 23 (46%) patients, respectively. Median progression‐free survival was 7.3 months and median overall survival was 11.9 months. All patients experienced AE and one or more grade 3/4 AE occurred in 43 of 50 (86%) patients. Although it requires close monitoring, sorafenib treatment seemed to be effective in the present study population.