bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autophagy induced by rapamycin and carbon‐starvation have distinct proteome profiles in Aspergillus nidulans
Authors: Kim Yonghyun , Islam Nazrul , Moss Bill J. , Nandakumar M. P. , Marten Mark R. 
Published: May 2011

AbstractIt is hypothesized that autophagy, a global catabolic pathway which is highly conserved from yeast to man, plays an important role in many bioprocesses. Though autophagy is known to be induced by either nutrient starvation or treatment with the drug rapamycin, it is not clear whether the two modes of induction have the same long‐term impact in the cell, particularly in the biotechnologically important filamentous fungi. Here, we compare the overall proteomes from the carbon‐starved (G−) and rapamycin treated (R+) model fungus Aspergillus nidulans. From about 1,100 visualized protein spots, we conservatively selected a total of 26 proteins with significant different expression. To highlight, increased levels of glucosidases and decreased levels of N‐acetylglucosamine pyrophosphorylase were observed, suggesting degradation of the fungal cell wall as an alternate carbon source for both modes of induction. Cdc37 was reduced in expression while 14‐3‐3 ArtA was increased, implying regulation of polar growth, while also potentially regulating autophagy negatively via PKA or Tor. Other proteins included aspartate transaminase, tryptophan synthase B (TrpB), glycylpeptide N‐tetradecanoyltransferase (Nmt1), and aldehyde dehydrogenase (aldA). More interestingly, the majority of the identified proteins (16 of 26) were uniquely expressed in elevated levels in G−. A novel predicted protein from AN8223 which has no sequence homology to other organisms is also implicated to be involved in carbon‐starvation. Thus, proteomic data here show that in A. nidulans, rapamycin‐induced autophagy and carbon‐starvation induced autophagy share some effectors for cell survival, but predominantly involve different long‐term effectors. Biotechnol. Bioeng. 2011;108: 2705–2715. © 2011 Wiley Periodicals, Inc.
Impact of anticoagulation on ionic and nonionic contrast media effect on thrombogenesis and fibrinolysis: The PEPCIT study
Authors: Bellemain‐Appaix Anne , Beygui Farzin , Lesty Claude , Gupta Satya , Silvain Johanne , Le Feuvre Claude , Cayla Guillaume , Allali Yann , Montalescot Gilles , Collet Jean‐Philippe 
Published: May 2011

AbstractObjectives: The effect of ionic low osmolar contrast media (ICM) and nonionic iso‐osmolar CM (NICM) on acute thrombotic complications of percutaneous coronary intervention (PCI) is subject to controversies possibly related to a potential interaction with anticoagulation regimens. We sought to compare physical and morphological properties of fibrin clots made in the presence of ioxaglate (ICM), iodixanol (NICM) versus control and to evaluate the effect of four anticoagulants used in PCI. Methods and Results: Maximum platelet aggregation (MPA%), maximum elastic modulus (EM, dyne/cm2) fiber density (n/10−5/μm2), and lysis front velocity (nm/sec) of fibrin rich clot (FRC) were measured simultaneously using peripheral blood from 12 patients undergoing elective PCI. We compared the effects of adding iodixanol or ioxaglate or saline (control) to blood with enoxaparin, unfractionated heparin, fondaparinux, and bivalirudin. Iodixanol and ioxaglate led to nonsignificant reduction in MPA compared to control (33.6% ± 16.9%, 28.2% ± 18.9%, and 40.7% ± 13.9%, respectively, P = ns). Fibrin formed with iodixanol was stiffer (42.7 ± 41.9, 18.7 ± 3.7, and 15.9 ± 9 dyne/cm2, P < 0.01) and displayed more fibrin fibers (1089 ± 175, 260 ± 108, and 456 ± 131 n/10−5/μm2, respectively, P < 0.01) than with ioxaglate or control. This resulted in a profound reduction in the lysis front velocity (191 ± 95, 261 ± 112, and 360 ± 153 nm/sec). None of the four anticoagulants displayed any significant interaction on the effect of contrast media. Conclusions: The prothrombogenic effect of iodixanol is related primarily to an increase in fibrin stiffness with subsequent delayed fibrinolysis, something not seen with ioxaglate. Anticoagulation does not appear to have any impact on this fibrin clot abnormalities. © 2011 Wiley‐Liss, Inc.
Mechanistic Insights into Ring‐Closing Enyne Metathesis with the Second‐Generation Grubbs–Hoveyda Catalyst: A DFT Study
Authors: Nuñez‐Zarur Francisco , Solans‐Monfort Xavier , Rodríguez‐Santiago Luis , Pleixats Roser , Sodupe Mariona 
Published: May 2011

AbstractThe full catalytic process (precatalyst activation, propagating cycle and active‐species interconversion) of the ring‐closing enyne metathesis (RCEYM) reaction of 1‐allyloxy‐2‐propyne with the Grubbs–Hoveyda complex as catalyst was studied by B3LYP density functional theory. Both the ene‐then‐yne and yne‐then‐ene pathways are considered and, for the productive catalytic cycle, the feasibility of the endo‐yne‐then‐ene route is also explored. Calculations predict that the ene‐then‐yne and yne‐then‐ene pathways proceed through equivalent steps, the only major difference being the order in which they take place. In this way, all alkene metathesis processes studied here involve four steps: olefin coordination, cycloaddition, cycloreversion and olefin decoordination. Among them, the two more energetically demanding ones are the olefin coordination and decoordination steps. The reaction of the alkyne fragment consists of two steps: alkyne coordination and alkyne skeletal reorganization, the latter of which has the highest Gibbs energy barrier. Comparison between the ene‐then‐yne and yne‐then‐ene pathways shows that there is no clear energetic preference for either of the two processes, and thus both should be operative when unsubstituted enynes are involved. In addition, although the endo orientation is computed to be slightly disfavored, it is not ruled out for 1‐allyloxy‐2‐propyne, and thus calculations seem to indicate that the exo versus endo selectivity is strongly influenced by the presence of substituents in the reagent.
The Crystal Structure of the CeNA:RNA Hybrid ce(GCGTAGCG):r(CGCUACGC)
Authors: Ovaere Margriet , Herdewijn Piet , Van Meervelt Luc 
Published: May 2011

AbstractCyclohexenyl nucleic acids (CeNA) are characterised by the carbon–carbon double bond replacing the O4′‐oxygen atom of the natural D‐2′‐deoxyribose sugar ring in DNA. CeNAs exhibit a high conformational flexibility, are stable against nuclease activity and their hybridisation is RNA selective. Additionally, CeNA has been shown to induce an enhanced biological activity when incorporated in siRNA. This makes CeNA a good candidate for siRNA and synthetic aptamer applications. The crystal structure of the synthetic CeNA:RNA hybrid ce(GCGTAGCG):r(CGCUACGC) has been solved with a resolution of 2.50 Å. The CeNA:RNA duplex adopts an anti‐parallel, right‐handed double helix with standard Watson–Crick base pairing. Analyses of the helical parameters revealed the octamer to form an A‐like double helix. The cyclohexenyl rings mainly adopt the 3H2 conformation, which resembles the C3′‐endo conformation of RNA ribose ring. This C3′‐endo ring puckering was found in most of the RNA residues and is typical for A‐family helices. The crystal structure is stabilised by the presence of hexahydrated magnesium ions. The fact that the CeNA:RNA hybrid adopts an A‐type double helical conformation confirms the high potential of CeNAs for the construction of efficient siRNAs which can be used for therapeutical applications.
Light‐Induced Water Oxidation by a Ru complex Containing a Bio‐Inspired Ligand
Authors: Kärkäs Markus D. , Johnston Eric V. , Karlsson Erik A. , Lee Bao‐Lin , Åkermark Torbjörn , Shariatgorji Mohammadreza , Ilag Leopold , Hansson Örjan , Bäckvall Jan‐E. , Åkermark Björn 
Published: May 2011

AbstractThe new Ru complex 8 containing the bio‐inspired ligand 7 was successfully synthesized and characterized. Complex 8 efficiently catalyzes water oxidation using CeIV and RuIII as chemical oxidants. More importantly, this complex has a sufficiently low overpotential to utilize ruthenium polypyridyl‐type complexes as photosensitizers.
Effect of fenoterol stereochemistry on the β2 adrenergic receptor system: Ligand‐directed chiral recognition
Authors: Jozwiak Krzysztof , Plazinska Anita , Toll Lawrence , Jimenez Lucita , Woo Anthony Yiu‐Ho , Xiao Rui‐Ping , Wainer Irving W. 
Published: May 2011

AbstractThe β2 adrenergic receptor (β2‐AR) is a model system for studying the ligand recognition process in G protein‐coupled receptors. Fenoterol (FEN) is a β2‐AR selective agonist that has two centers of chirality and exists as four stereoisomers. Radioligand binding studies determined that stereochemistry greatly influences the binding affinity. Subsequent Van't Hoff analysis shows very different thermodynamics of binding depending on the stereoconfiguration of the molecule. The binding of (S,x′)‐isomers is almost entirely enthalpy controlled whereas binding of (R,x′)‐isomers is purely entropy driven. Stereochemistry of FEN molecule also affects the coupling of the receptor to different G proteins. In a rat cardiomyocyte contractility model, (R,R′)‐FEN was shown to selectively activate Gs protein signaling while the (S,R′)‐isomer activated both Gi and Gs protein. The overall data demonstrate that the chirality at the two chiral centers of the FEN molecule influences the magnitude of binding affinity, thermodynamics of local interactions within the binding site, and the global mechanism of β2‐AR activation. Differences in thermodynamic parameters and nonuniform G‐protein coupling suggest a mechanism of chiral recognition in which observed enantioselectivities arise from the interaction of the (R,x′)‐FEN stereoisomers with a different receptor conformation than the one with which the (S,x′)‐isomer interacts. Chirality 2011. © 2011 Wiley‐Liss, Inc.
MOCVD of ZnO Films from Bis(Ketoiminato)Zn(II) Precursors: Structure, Morphology and Optical Properties
Authors: Bekermann Daniela , Ludwig Arne , Toader Teodor , Maccato Chiara , Barreca Davide , Gasparotto Alberto , Bock Claudia , Wieck Andreas D. , Kunze Ulrich , Tondello Eugenio , Fischer Roland A. , Devi Anjana 
Published: May 2011

AbstractTwo closely related bis(ketoiminato) zinc precursors, which are air stable and possess favorable properties for metal‐organic (MO)CVD, are successfully employed for the growth of ZnO films on silicon and borosilicate glass substrates at temperatures between 400 and 700 °C. The as‐deposited films are investigated by X‐ray diffraction (XRD), field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X‐ray photoelectron spectroscopy (XPS), nuclear reaction analysis (NRA), as well as by UV‐vis absorption spectroscopy and photoluminescence (PL) measurements. The structure, morphology, and composition of the as‐grown films show a strong dependence on the substrate temperature. The formation of pure and (001)‐oriented wurtzite‐type stoichiometric ZnO is observed. PL measurements are performed both at room temperature and 77 K, revealing a defect‐free emission of ZnO films.
Early transcriptional regulation by C‐peptide in freshly isolated rat proximal tubular cells
Authors: Lindahl Emma , Nordquist Lina , Müller Patrick , El Agha Eli , Friederich Malou , Dahlman‐Wright Karin , Palm Fredrik , Jörnvall Hans 
Published: May 2011

AbstractBackgroundClinical studies have shown that proinsulin C‐peptide exerts renoprotective effects in type 1 diabetes, although the underlying mechanisms are poorly understood. As C‐peptide has been shown to induce several intracellular events and to localize to nuclei, we aimed to determine whether gene transcription is affected in proximal tubular kidney cells, and if so, whether the genes with altered transcription include those related to protective mechanisms.MethodsThe effect of C‐peptide incubation (2 h) on gene expression was investigated in freshly isolated proximal tubular cells from streptozotocin‐diabetic Sprague‐Dawley rats using global gene expression profiling and real‐time quantitative polymerase chain reaction. Protein expression was assayed using western blotting. Different bioinformatic strategies were employed.ResultsGene transcription profiling demonstrated differential transcription of 492 genes (p < 0.01) after 2 h of C‐peptide exposure, with the majority of these genes repressed (83%). Real‐time quantitative polymerase chain reaction validation supported a trend of several G protein‐coupled receptors being activated, and certain transcription factors being repressed. Also, C‐peptide repressed the transcription of genes associated with the pathways of circulatory and inflammatory diseases.ConclusionThis study shows that C‐peptide exerts early effects on gene transcription in proximal tubular cells. The findings also bring further knowledge to the renoprotective mechanisms of C‐peptide in type 1 diabetes, and support a transcriptional activity for C‐peptide. It is suggested that C‐peptide may play a regulatory role in the gene expression of proximal tubular cells. Copyright © 2011 John Wiley & Sons, Ltd.
Phosphorus‐Centered and Phosphinidene‐Capped Tungsten Chloride Clusters
Authors: Ströbele Markus , Eichele Klaus , Meyer H.‐Jürgen 
Published: May 2011

AbstractBlack crystalline powders of W6PCl17 and W4(PCl)Cl10 were obtained after reducing WCl6 with red phosphorus at 370 and 500 °C. The crystal structures were determined by single‐crystal and powder X‐ray diffraction analyses. The structure of W6PCl17 is represented by a phosphorus‐centered hexanuclear tungsten cluster, whose (W6PCl11)Cl4aCl4/2a–a chains form a hexagonal stick packing structure. The structure of W4(PCl)Cl10 is represented by a Jahn–Teller distorted tetranuclear tungsten cluster that is interconnected into a layered [W4(μ4‐PCl)Cl6i]Cl8/2a–a structure containing a chloro‐phosphinidene ligand. Solid‐state 31P magic‐angle spinning (MAS) NMR spectra, electronic structures, and magnetic properties are reported.
Racial identification modulates default network activity for same and other races
Authors: Mathur Vani A. , Harada Tokiko , Chiao Joan Y. 
Published: May 2011

AbstractRacial identification shapes self‐concept and how people share in and respond to the emotional states of others around them. Prior neuroimaging studies have demonstrated the role of the neural default network in self‐referential and empathic processing. However, how racial identification affects neural processing of social information remains unknown. Here, we examined the effect of racial identification on neural response related to social perception among African American and Caucasian American individuals using functional magnetic resonance imaging. Our results demonstrate that degree of racial identification predicts activity within cortical midline structures of the default network in response to viewing racial ingroup, relative to outgroup members, and activity within the medial temporal lobe subsystem of the default network in response to viewing racial outgroup, relative to ingroup members. Broadly, our findings suggest that the strength of racial identification is associated with differential recruitment of neural and cognitive processes to understand and respond to other people within and outside of one's racial group. Hum Brain Mapp, 2011. © 2011 Wiley‐Liss, Inc.
Measurement of spectra of normal incidence absorptance of surfaces in life space
Authors: Wakabayashi Hidenobu , Makino Toshiro 
Published: May 2011

AbstractThe thermal and radiative environment of human life space is characterized strongly by solar radiation and by the radiation from surrounding walls as well as by air temperature and humidity. To consider the environmental radiation quantitatively, absorptance spectra and total emittance of the surfaces should be measured properly. In this work we measure spectra of normal incidence absorptance of such surfaces in a near‐ultraviolet through infrared region of wavelength of 0.30–11 μm. On the basis of measured spectra we evaluate solar absorptance of the surfaces to respond to the needs of radiation characteristics of the surfaces in the fields of architecture, life science, and solar engineering. Objective surfaces of the measurement are surfaces of indoor and outdoor environments: cloths, indoor wall materials, painted surfaces, ceramics, bricks, outdoor wall materials, road materials, leaves, etc. We also observe the transition of the absorptance spectra of a water–wet cement surface in a drying process. © 2011 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.20356
High‐risk human papillomavirus in oral squamous cell carcinoma of young patients
Authors: Kaminagakura Estela , Villa Luísa Lina , Andreoli Maria Antonieta , Sobrinho João Simão , Vartanian José Guilherme , Soares Fernando Augusto , Nishimoto Inês Nobuko , Rocha Rafael , Kowalski Luiz Paulo 
Published: May 2011

AbstractThe aim of this study was to investigate a possible relation between oral squamous cell carcinoma (SCC), the presence of high‐risk human papillomavirus (HR‐HPV) DNA and p16 expression in young patients. Paraffin‐embedded tumor blocks from 47 oral SCC of young (≤40‐year old) patients were evaluated. The presence of HPV DNA in tumor specimens was analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ generic primers (L1 region) followed by dot blot hybridization for HPV typing. When necessary, the HPV16 positivity was confirmed by PCR HPV16 E7‐specific primers. Cases involving young patients were compared with 67 oral SCC from patients ≥50‐year old (controls). Demographic and clinical data were collected to analyze patient outcomes. p16ink4 expression was evaluated by immunostaining of tissue microarrays. HPV16 was detected in 22 (19.2%) cases; 15 (68.2%) young and 7 (31.8%) control patients, a statistically significant difference (p = 0.01). In 1 (1.7%) young group specimen, HPV DNA 16 and 18 was detected. p16 expression was observed in 11 (25.6%) cases from the young group and in 11 (19.6%) controls (p = 0.48). Association between HPV and p16 was verified, and it was statistically significant (p = 0.002). The higher prevalence of high‐risk HPV types, especially HPV16, may be a contributing factor to oral carcinogenesis in younger individuals.
Carcinogen metabolism genes, red meat and poultry intake, and colorectal cancer risk
Authors: Wang Jun , Joshi Amit D. , Corral Román , Siegmund Kimberly D. , Marchand Loïc Le , Martinez Maria Elena , Haile Robert W. , Ahnen Dennis J. , Sandler Robert S. , Lance Peter , Stern Mariana C. 
Published: May 2011

AbstractDiets high in red meat are established risk factors for colorectal cancer (CRC). Carcinogenic compounds generated during meat cooking have been implicated as causal agents. We conducted a family‐based case‐control study to investigate the association between polymorphisms in carcinogen metabolism genes (CYP1A2 ‐154A>C, CYP1B1 Leu432Val, CYP2E1 ‐1054C>T, GSTP1 Ile105Val, PTGS2 5UTR ‐765, EPHX1 Tyr113His, NAT2 Ile114Thr, NAT2 Arg197Gln and NAT2 Gly286Glu) and CRC risk. We tested for gene‐environment interactions using case‐only analyses (N = 577) and compared statistically significant results to those obtained using case‐unaffected sibling comparisons (N = 307 sibships). Our results suggested that CYP1A2 ‐154A>C might modify the association between intake of red meat cooked using high temperature methods and well done on the inside and CRC risk (case‐only interaction OR = 1.53; 95% CI = 1.19–1.97; p = 0.0008) and the association between intake of red meat heavily browned on the outside and rectal cancer risk (case‐only interaction OR = 0.65; 95% CI = 0.48–0.86; p = 0.003). We also found that GSTP1 Ile105Val might modify the association between intake of poultry cooked with high temperature methods and CRC risk (p = 0.0035), a finding that was stronger among rectal cancer cases. Our results support a role for heterocyclic amines that form in red meat as a potential explanation for the observed association between diets high in red meat and CRC. Our findings also suggest a possible role for diets high in poultry cooked at high temperatures in CRC risk.
MAGE‐D4B is a novel marker of poor prognosis and potential therapeutic target involved in breast cancer tumorigenesis
Authors: Germano Serena , Kennedy Susan. , Rani Sweta , Gleeson Grainne , Clynes Martin , Doolan Padraig , McDonnell Susan , Hughes Linda , Crown John , O'Driscoll Lorraine 
Published: May 2011

AbstractMelanoma‐associated antigen (MAGE) family members are generally described as tumor‐specific antigens. An association between MAGE‐D4B and breast cancer has yet to be reported and the functional role of the encoded protein has never been established. We performed microarray analysis of 104 invasive breast tumors and matched non‐cancerous breast biopsies. qPCR was used for validation in an independent biobank. To investigate the biological relevance of MAGE‐D4B in breast tumorigenesis, its phenotypic effects were assessed in vitro. Overall, MAGE‐D4B was detected in 43% of tumors while undetected in normal breast tissue. MAGE‐D4B was found to correlate with tumor progression and to be an independent prognostic marker for poor outcome in term of relapse‐free and overall survival, with potential predictive relevance in relation to response to chemotherapy. RNA interference‐mediated knockdown of MAGE‐D4B significantly hampered the invasive properties of Hs578T cells by affecting anchorage‐independent growth, adhesion, migration and invasion affecting anchorage‐independent growth, adhesion, migration and invasion and by modulating expression of invasion‐suppressor gene E‐cadherin.
Comparison of hematopoietic progenitor cell collections using the COBE Spectra version 7 and Amicus version 3.1 for patients with al amyloidosis
Authors: Burgstaler Edwin A. , Winters Jeffrey L. 
Published: May 2011

AbstractTo address concerns about infused fluid volume during HPC collections in patients with AL amyloidosis, our institution has used a 26:1 anticoagulant (AC) ratio on the COBE Spectra and on the Fenwal Amicus. In this study, in a cohort of AL amyloid patients, we compared the Amicus version 3.1 to the Spectra version 7 MNC collections with regard to infused fluid volume, CD34+ cell yield, lymphocyte yield, cross‐cellular content, and adverse reactions. Both instruments used a 26:1 AC ratio but the Amicus delivered significantly less AC per procedure (Amicus 678 mL vs. Spectra 753 mL). With comparable baseline CD34+ cell counts (Amicus 33 cells/μL vs. Spectra 27 cells/μL); Amicus collected significantly more CD34+ cells (3.1 vs. 1.5 × 106/kg) and equivalent lymphocytes (18.7 vs. 14.5 × 109). Amicus collected significantly fewer WBC (51.8 vs. 72.7 × 109), granulocytes (15.1 vs. 27.5 × 109), and PLT (2.3 vs. 3.9 × 1011) per procedure with equivalent RBC content (26 vs. 30 mL). CD34+ cell (5.0 vs. 4.4 × 106/kg) and lymphocyte doses (32.7 vs. 33.9 × 109) were equivalent in infused products collected on the Amicus and Spectra but the frequency of high volume products was lower for Amicus. Frequency and severity of adverse reactions during collection and infusion were similar for both. In this group of AL amyloid patients, Amicus was superior to Spectra with regard to fluid infused, CD34+ cell yield, and cross‐cellular contamination with equivalent lymphocyte yield and reaction incidence. © 2011 Wiley‐Liss, Inc.