bluuebunny/crossref_metadata_2025_split huggingface dataset:
Somatic Deletions of the PolyA Tract in the 3′ Untranslated Region of Epidermal Growth Factor Receptor Are Common in Microsatellite Instability–High Endometrial and Colorectal Carcinomas
Authors: Deqin Ma , Chen Zhao , Nero Christopher , Patel Keyur P. , Daoud Emad M. , Cheng Hanyin , Djordjevic Bojana , Broaddus Russell R. , Medeiros L Jeffrey , Rashid Asif , Luthra Rajyalakshmi 
Published: April 2012

Context.—Epidermal growth factor receptor (EGFR) is overexpressed in up to 80% of colorectal and endometrial carcinomas. Deletions of the polyA tract in the 3′ untranslated region (3′ UTR) have been reported in microsatellite instability–high (MSI-H) colonic carcinomas, but their impacts on EGFR expression and downstream pathways are unclear. This phenomenon has not been reported in other MSI-H tumors.
Objective.—To assess the 3′ UTR polyA tract of EGFR in both endometrial and colorectal carcinomas and the mutational status of EGFR downstream pathways.
Design.—Ninety-eight colorectal carcinomas and 47 endometrial carcinomas were included. EGFR 3′ UTR polyA status was detected by capillary electrophoresis and Sanger sequencing. EGFR gene expression, EGFR copy numbers, and KRAS and BRAF mutation status were analyzed accordingly.
Results.—The 3′ UTR polyA tract was deleted in 18 of 23 (78%) MSI-H versus 0 of 24 microsatellite-stable endometrial carcinomas (P &lt; .001). Similar observations were seen in colorectal carcinomas, in which 29 of 36 (81%) MSI-H, 1 of 62 (1.6%) microsatellite instability–low, and none of the microsatellite-stable tumors harbored the deletion (P &lt; .001). A moderate increase in EGFR mRNA level was observed in endometrial carcinomas with 3′ UTR polyA deletions versus those with wild-type polyA tract. Amplification of the EGFR gene was not observed. Deletions in polyA tract do not seem to affect the frequency of KRAS and BRAF mutations.
Conclusions.—Deletions of EGFR 3′ UTR polyA are frequent in endometrial and colorectal carcinomas, are confined almost exclusively to MSI-H tumors, and do not affect KRAS and BRAF mutations.
Multiphoton Microscopy in the Evaluation of Human Bladder Biopsies
Authors: Jain Manu , Robinson Brian D. , Scherr Douglas S. , Sterling Joshua , Lee Ming-Ming , Wysock James , Rubin Mark A. , Maxfield Frederick R. , Zipfel Warren R. , Webb Watt W. , Mukherjee Sushmita 
Published: April 2012

Context.—Multiphoton microscopy (MPM) is a nonlinear imaging approach, providing cellular and subcellular details from fresh (unprocessed) tissue by exciting intrinsic tissue emissions. With miniaturization and substantially decreased cost on the horizon, MPM is an emerging imaging technique with many potential clinical applications.Objectives.—To assess the imaging ability and diagnostic accuracy of MPM for human bladder biopsies.Design.—Seventy-seven fresh bladder biopsies were imaged by MPM and subsequently submitted for routine surgical pathology diagnosis. Twelve cases were excluded because of extensive cautery artifact that prohibited definitive diagnosis. Comparison was made between MPM imaging and gold standard sections for each specimen stained with hematoxylin-eosin.Results.—In 57 of 65 cases (88%), accurate MPM diagnoses (benign or neoplastic) were given based on the architecture and/or the cytologic grade. The sensitivity and specificity of MPM in our study were 90.4% and 76.9%, respectively. A positive (neoplastic) diagnosis on MPM had a high predictive value (94%), and negative (benign) diagnoses were sustained on histopathology in two-thirds of cases. Architecture (papillary versus flat) was correctly determined in 56 of 65 cases (86%), and cytologic grade (benign/low grade versus high grade) was assigned correctly in 38 of 56 cases (68%).Conclusions.—The MPM images alone provided sufficient detail to classify most lesions as either benign or neoplastic using the same basic diagnostic criteria as histopathology (architecture and cytologic grade). Future developments in MPM technology may provide urologists and pathologists with additional screening and diagnostic tools for early detection of bladder cancer. Additional applications of such emerging technologies warrant exploration.
Renal Myopericytoma: Case Report and Review of Literature
Authors: Dhingra Sadhna , Ayala Alberto , Chai Hong , Moreno Vanessa , Zhao Bihong 
Published: April 2012

Myopericytoma arising in the visceral organs is rare and only 1 case of renal myopericytoma has been reported in the literature to date. We report the second case of myopericytoma arising in the kidney in a 40-year-old Hispanic woman who presented with pain on the left side of the abdomen and frequent urination. Abdominal computed tomography scan showed an exophytic left-sided renal mass. Partial nephrectomy was performed. The patient remains free of disease at 24 months after diagnosis. Our case is histologically distinct from the previously described case as it lacks the “hemangiopericytic/glomangiopericytoma” pattern. The tumor in our case showed the characteristic pattern of myopericytoma and an additional glomus tumorlike pattern. The tumor cells showed diffuse reactivity for vimentin, smooth muscle actin, smooth muscle myosin heavy chain, and muscle-specific actin in both morphologic patterns and strong diffuse CD34 expression in glomus tumorlike focus. This case report adds to the morphologic heterogeneity of myopericytomas.
Recent Trends in Aluminum Foam Sandwich Technology
Authors: Banhart John , Seeliger Hans‐Wolfgang 
Published: April 2012

AbstractWe review the status of aluminum foam sandwich (AFS) technology and discuss both recent improvements of foaming technology and current application strategies. It is concluded that the quality of foams has improved in the past years but the costs are still very much the same. This is why applications in which metal foams have more than one function are more likely to be economically viable. The examples presented include electromagnetic shielding, carrier plates for mirrors, cooking equipment, architectural panels, and blast protection.
Extension of Ashby's Performance Indexes in Mixed Tension‐Bending Solicitations
Authors: Bouaziz Olivier , Masse Jean‐Philippe 
Published: April 2012

AbstractAshby's performance indexes are a fundamental tool for material selection especially for structures lightening. Unfortunately the indexes are available only for simple mechanical solicitation as pure bending or pure tension. For real applications, it is required to have a performance index for combined solicitations. This publication proposes an approach to develop this kind of extended performance index and shows the exploitation in order to compare materials performance in more realistic situations.
Magnetic Nanoparticles‐Supported Palladium: A Highly Efficient and Reusable Catalyst for the Suzuki, Sonogashira, and Heck Reactions
Authors: Li Pinhua , Wang Lei , Zhang Lei , Wang Guan‐Wu 
Published: April 2012

AbstractA highly efficient, air‐ and moisture‐stable and easily recoverable magnetic nanoparticle‐supported palladium catalyst has been developed for the Suzuki, Sonogashira and Heck reactions. A wide range of substrates was coupled successfully under aerobic conditions. In particular, the performance of the magnetic separation of the catalyst was very efficient, and it is possible to recover and reuse it at least eight times without significant loss of its catalytic activity.
Cyclodextrin/Amphiphilic Phosphane Mixed Systems and their Applications in Aqueous Organometallic Catalysis
Authors: Ferreira Michel , Bricout Hervé , Azaroual Nathalie , Landy David , Tilloy Sébastien , Hapiot Frédéric , Monflier Eric 
Published: April 2012

AbstractWhen mixed with a water‐soluble phosphane capable of self‐assembling into micelles, native or modified β‐cyclodextrins (β‐CDs) show very contrasting behavior depending on their neutral or ionic nature. In the post‐micellar region, neutral β‐CDs led to a micelle destructuring. Conversely, micelles remained stable over a well‐defined range of ionic β‐CD concentrations. In that case, the micelle destruction was only observed when using a large excess of ionic β‐CDs. The catalytic performances of these micellar systems have been evaluated in a rhodium‐catalyzed hydroformylation reaction of 1‐decene. We showed that, using ionic β‐CDs, the catalytic activity could be improved without a detrimental impact upon the regioselectivity. A linear/branched aldehyde ratio as high as 8.6 could be achieved. The best results were obtained with stoichiometric quantities of ionic randomly methylated β‐CDs with respect to the phosphane with a beneficial effect on the decantation at the end of the reaction.
An Unexpected Synthesis of Dihydrophenazines en Route to Benzimidazolium Salts
Authors: Borguet Yannick , Zaragoza Guillermo , Demonceau Albert , Delaude Lionel 
Published: April 2012

AbstractThe oxidation of various N,N′‐diarylbenzene‐1,2‐diamines bearing bulky aromatic substituents with sodium periodate on wet silica gel afforded a series of five new dihydrophenazines instead of the expected cyclohexadiene‐1,2‐diimines. The reaction most likely proceeds via a 1,6‐electrocyclic path and provides a convenient access to an important class of nitrogen heterocycles. Subsequent treatment of the mesityl derivative with chloromethyl pivalate and silver triflate led to the corresponding benzimidazolium salt.
Development of a Palladium on Boron Nitride Catalyst and its Application to the Semihydrogenation of Alkynes
Authors: Yabe Yuki , Yamada Tsuyoshi , Nagata Saori , Sawama Yoshinari , Monguchi Yasunari , Sajiki Hironao 
Published: April 2012

AbstractThe simple preparative method for a novel palladium supported on boron nitride catalyst (Pd/BN) was accomplished. Pd/BN is widely applicable for the semihydrogenation of mono‐ as well as disubstituted alkynes to furnish the corresponding alkenes in the presence of diethylenetriamine (DETA), which exhibits both an unprecedented acceleration effect toward the semihydrogenation and a suppression effect with regard to the overhydrogenation to alkanes.
Effect of the Helical Tether of a Resin‐Supported Peptide Catalyst for Friedel–Crafts‐Type Alkylation in Water
Authors: Akagawa Kengo , Suzuki Rieko , Kudo Kazuaki 
Published: April 2012

AbstractA detailed investigation of the helical part of the resin‐supported peptide catalyst possessing a turn motif and helical unit was conducted to clarify the structure‐activity relationship. The peptide catalyst with an α‐ or 310‐helical tether was effective for an enantioselective Friedel–Crafts‐type alkylation in water. From the spectral analysis of the peptide with an optimum sequence, it was demonstrated that the helical moiety of the peptide catalyst played a role for stabilizing a terminal turn structure.
Early landmark analysis of imatinib treatment in CML chronic phase: Less than 10% BCR‐ABL by FISH at 3 months associated with improved long‐term clinical outcome
Authors: Ohm Lotta , Arvidsson Ingrid , Barbany Gisela , Hast Robert , Stenke Leif 
Published: April 2012

AbstractImatinib has dramatically improved the clinical outcome in chronic myeloid leukemia, chronic phase (CMLcp), but a risk of resistance and serious disease progression still prevails. We have studied 45 newly diagnosed CMLcp patients initiated on imatinib, assessing treatment responses by interphase extral signal (ES)‐fluorescence in situ hybridization (FISH), quantitative real‐time (q‐RT) polymerase chain reaction (PCR), and chromosome banding analysis. In a landmark analysis, an early favorable response, defined as less than 10% BCR‐ABL‐positive cells by FISH after 3 months of treatment, was identified as a predictive marker of an improved long‐term clinical outcome. Of evaluable patients, 51% achieved this response. A large majority, 95% of such responders reached complete cytogenetic responses (CCyR) within 12 months and 100% event‐free survival (EFS) at 48 months, when compared with 67 and 65%, respectively, of patients with higher breakpoint cluster region ‐ Abelson (BCR‐ABL) positivity at 3 months (P = 0.04; P = 0.006). No similar, significant correlations were noted between early disease assessments with PCR of BCR‐ABL mRNA transcripts or of cytogenetics versus a 12‐month CCyR or long‐term EFS. Our data, based on a limited patient cohort, indicate that (i) FISH can effectively be used in the early assessment of remaining Ph‐positive cells to identify patients at risk for a long‐term nonoptimal response to imatinib and that (ii) FISH may be more useful than PCR for this purpose. Am. J. Hematol., 2012. © 2012 Wiley Periodicals, Inc.
Genetic polymorphisms in IL10RA and TNF modify the association between blood transfusion and risk of non‐Hodgkin lymphoma
Authors: Bi Xiaofeng , Zheng Tongzhang , Lan Qing , Xu Zhijian , Chen Yingtai , Zhu Gongjian , Foss Francine , Kim Christopher , Dai Min , Zhao Ping , Holford Theodore , Leaderer Brian , Boyle Peter , Deng Qian , Chanock Stephen J. , Rothman Nathaniel , Zhang Yawei 
Published: April 2012

AbstractWe conducted a population‐based case‐control study in Connecticut women to test the hypothesis that genetic variations in Th1 and Th2 cytokine genes may modify the association between blood transfusion and risk of non‐Hodgkin lymphoma (NHL). Compared with women without blood transfusion, women with a history of transfusion had an increased risk of NHL if they carried IL10RA (rs9610) GG genotype [odds ratio (OR) = 1.9, 95% confidence interval (CI): 1.1–3.2] or TNF (rs1800629) AG/AA genotypes (OR = 1.6, 95% CI: 0.9–2.7). We also found women with a history of transfusion had a decreased risk of NHL if they carried IL10RA (rs9610) AG/AA genotypes (OR = 0.6, 95% CI: 0.4–0.9) or TNF (rs1800629) GG genotype (OR = 0.7, 95% CI: 0.5–1.0). A similar pattern was also observed for B‐cell lymphoma but not for T‐cell lymphoma. Statistically significant interactions with blood transfusion were observed for IL10RA (rs9610) (Pforinteraction = 0.003) and TNF (rs1800629) (Pforinteraction = 0.012) for NHL overall and IL10RA (rs9610) (Pforinteraction = 0.001) and TNF (rs1800629) (Pforinteraction = 0.019) for B‐cell lymphoma. The results suggest that genetic polymorphisms in TNF and IL10RA genes may modify the association between blood transfusion and NHL risk. Am. J. Hematol. 2012. © 2012 Wiley Periodicals, Inc.
Improving the specificity of the PF4 ELISA in diagnosing heparin‐induced thrombocytopenia
Authors: McFarland Janice , Lochowicz Andrew , Aster Richard , Chappell Bryan , Curtis Brian 
Published: April 2012

AbstractHeparin induced thrombocytopenia (HIT) is a serious complication of heparin therapy. The PF4 ELISA is a serologic assay that provides laboratory support for the clinical diagnosis of HIT, but it is often positive in patients who do not have the syndrome. We examined whether the specificity of the PF4 ELISA can be improved by 1) taking antibody potency into consideration, 2) by measuring only IgG antibodies, and 3) by utilizing a high concentration heparin inhibition step. We reviewed clinical information on 116 patients whose samples were referred for HIT antibody testing and assigned each a clinical score related to the likelihood of the patient having HIT. The scores were then correlated with serologic findings. Patients with strongly positive PF4ELISA results (OD ≥ 1.0) using both versions of the assay (IgG/A/M and IgG only) had clinical scores and SRA activity that were significantly higher than those having reactive or negative results. When the IgG‐only PF4 ELISA was used, only the strongly positive result group had significantly higher clinical scores and SRA release, and fewer samples were classified as weakly positive or reactive, suggesting that detection of IgG only in the PF4 ELISA improves the assay's specificity. The heparin inhibition step identified “reactive” samples that were associated with clinical scores and SRA release indistinguishable from the “negative” result groups, confirming that this step further improves specificity of the test. This study supports utilizing these 3 modifications of the PF4 ELISA to improve specificity in supporting the clinical diagnosis of HIT. Am. J. Hematol., 2012. © 2012 Wiley Periodicals, Inc.
Dietary intakes of berries and flavonoids in relation to cognitive decline
Authors: Devore Elizabeth E. , Kang Jae Hee , Breteler Monique M. B. , Grodstein Francine 
Published: April 2012

AbstractObjective:Berries are high in flavonoids, especially anthocyanidins, and improve cognition in experimental studies. We prospectively evaluated whether greater long‐term intakes of berries and flavonoids are associated with slower rates of cognitive decline in older women.Methods:Beginning in 1980, a semiquantitative food frequency questionnaire was administered every 4 years to Nurses' Health Study participants. In 1995–2001, we began measuring cognitive function in 16,010 participants, aged ≥70 years; follow‐up assessments were conducted twice, at 2‐year intervals. To ascertain long‐term diet, we averaged dietary variables from 1980 through the initial cognitive interview. Using multivariate‐adjusted, mixed linear regression, we estimated mean differences in slopes of cognitive decline by long‐term berry and flavonoid intakes.Results:Greater intakes of blueberries and strawberries were associated with slower rates of cognitive decline (eg, for a global score averaging all 6 cognitive tests, for blueberries: p‐trend = 0.014 and mean difference = 0.04, 95% confidence interval [CI] = 0.01–0.07, comparing extreme categories of intake; for strawberries: p‐trend = 0.022 and mean difference = 0.03, 95% CI = 0.00–0.06, comparing extreme categories of intake), after adjusting for multiple potential confounders. These effect estimates were equivalent to those we found for approximately 1.5 to 2.5 years of age in our cohort, indicating that berry intake appears to delay cognitive aging by up to 2.5 years. Additionally, in further supporting evidence, greater intakes of anthocyanidins and total flavonoids were associated with slower rates of cognitive decline (p‐trends = 0.015 and 0.053, respectively, for the global score).Interpretation:Higher intake of flavonoids, particularly from berries, appears to reduce rates of cognitive decline in older adults. ANN NEUROL 2012
A common polymorphism near PER1 and the timing of human behavioral rhythms
Authors: Lim Andrew S. P. , Chang Anne‐Marie , Shulman Joshua M. , Raj Towfique , Chibnik Lori B. , Cain Sean W. , Rothamel Katherine , Benoist Christophe , Myers Amanda J. , Czeisler Charles A. , Buchman Aron S. , Bennett David A. , Duffy Jeanne F. , Saper Clifford B. , De Jager Philip L. 
Published: April 2012

AbstractObjective:Circadian rhythms influence the timing of behavior, neurological diseases, and even death. Rare mutations in homologs of evolutionarily conserved clock genes are found in select pedigrees with extreme sleep timing, and there is suggestive evidence that certain common polymorphisms may be associated with self‐reported day/night preference. However, no common polymorphism has been associated with the timing of directly observed human behavioral rhythms or other physiological markers of circadian timing at the population level.Methods:We performed a candidate gene association study with replication, evaluating associations between polymorphisms in homologs of evolutionarily conserved clock genes and the timing of behavioral rhythms measured by actigraphy. For validated polymorphisms, we evaluated associations with transcript expression and time of death in additional cohorts.Results:rs7221412, a common polymorphism near period homolog 1 (PER1), was associated with the timing of activity rhythms in both the discovery and replication cohorts (joint p = 2.1 × 10−7). Mean activity timing was delayed by 67 minutes in rs7221412GG versus rs7221412AA homozygotes. rs7221412 also showed a suggestive time‐dependent relationship with both cerebral cortex (p = 0.05) and CD14+CD16− monocyte (p = 0.02) PER1 expression and an interesting association with time of death (p = 0.015) in which rs7221412GG individuals had a mean time of death nearly 7 hours later than rs7221412AA/AG.Interpretation:A common polymorphism near PER1 is associated with the timing of human behavioral rhythms, and shows evidence of association with time of death. This may be mediated by differential PER1 expression. These results may facilitate individualized scheduling of shift work, medical treatments, or monitoring of vulnerable patient populations. ANN NEUROL 2012;72:324–334.
Parametric studies on the synthesis of amidoximated adsorbent resins
Authors: Rafiee R. , Babalou A. A. , Nilchi A. , Razavi Aghjeh M. K. 
Published: April 2012

AbstractThe main goal of this study was to obtain acrylonitrile (AN)‐divinylbenzene (DVB) copolymer beads that can serve as the matrix for preparation of amidoxime resins. AN‐DVB amidoxime resin was synthesized via suspension polymerization and the effect of different parameters such as the speed of stirrer, the amounts of suspending agent, hydrophilic agent, diluent agent, and initiator on its properties was investigated by fractional factorial (Taguchi) experimental design method. This method gives much‐reduced variance for the experiments with optimum control parameters setting and provides a set of minimum experiments compared to the conventional methods. The results showed that the most effective parameters on the amidoximation of resins were hydrophilic agent content, speed of stirrer, and the amount of suspending agent, respectively. Furthermore, anion‐exchange capacity of the amidoxime resins was utilized as a criterion for the evaluation of amidoxime adsorbent groups and the adsorption potential of the synthesized resins was determined by cation‐exchange capacity. The amount of methylacrylate as a hydrophilic agent had the most significant effect on the ion‐exchange capacity of the final product. Swelling ratio was also measured to evaluate the adsorption capacity of the synthesized resin. The results showed that the amounts of hydrophilic and diluent agents had significant effects on swelling ratio of resin. Finally, cation‐exchange capacity and swelling ratio of amidoxime resin were changed greatly because of alkaline treatment, but it had no significant effect on the anion‐exchange capacity of the synthesized resin. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
The influence of solvent type and polymer concentration on the physical properties of solid state polymerized PA66 nanofiber yarn
Authors: Sanatgar Razieh Hashemi , Borhani Sedigheh , Ravandi Seyed Abdolkarim Hosseini , Gharehaghaji Ali Akbar 
Published: April 2012

AbstractWe investigated the effects of two different solvent types and three solution concentrations on the electrospinning of solid state polymerized polyamide 66 (SSP PA66) nanofiber yarns. Nanofiber yarns were electrospun from SSP PA66 solutions in formic acid and formic acid/chloroform (3/1), using two oppositely metallic spinnerets system. Scanning electron microscopy (SEM) and X‐ray diffraction (XRD) were employed to characterize the morphology and properties of the nanofibrous yarns. Experimental results show that adding chloroform to formic acid as a binary solvent increases viscosity of polymer solution and the nanofibers diameter significantly. XRD patterns reveal that the presence of chloroform affects the crystallinity and the mechanical properties of the produced nanofibrous yarns. PA66 nanofiber yarn from 10 wt % formic acid/chloroform (3/1) solution was successfully electrospun with strength and modulus of 120.16 MPa and 1216.27 MPa respectively. It is also shown that the solution concentration has a significant effect on the modulus of the nanofibers yarns. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012