bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary evaluation of Hitchens–Pike–Todd–Hewitt medium (HPTH) for detection of group B streptococci in pregnant women
Authors: Chaves Maurício , Pádua Rubia A.F. , Campanerut Paula A.Z. , Pelloso Sandra M. , Carvalho Maria Dalva B. , Siqueira Vera L.D. , Scodro Regiane B.L. , Cardoso Rosilene F. 
Published: November 2010

AbstractWe aimed to compare Hitchens–Pike–Todd–Hewitt (HPTH) enrichment broth, Todd–Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericórdia Hospital, Maringá, Paraná, Brazil, were screened by GBS using HPTH, Todd–Hewitt broth, and ABA. Twenty‐five(24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd–Hewitt broth. HPTH seems to be a high satisfactory medium for screening GBS in vaginal and anorectal samples in pregnant women and additional studies would be interesting in a larger number of pregnant women and in different laboratories. J. Clin. Lab. Anal. 24:403–406, 2010. © 2010 Wiley‐Liss, Inc.
Evaluation of BD vacutainer SST™ II plus tubes for common tumor marker tests by Roche Diagnostics Modular E 170 analyzer
Authors: Li Zhiyan , Feng Zhenru , Yan Cunling , Yan Rong 
Published: November 2010

AbstractSerum separator tubes were introduced 35 years ago and were widely used in the clinical laboratory in China for routine collection of blood because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens. This type of tubes facilitated rapid separation of serum from cellular constituents of blood and also prevented hemolysis upon prolonged storage. However, there were some limitations associated with gel tubes (i.e., gel and analyte stability). In order to circumvent these problems, BD released a new serum separator tube containing a new gel (BD SST™ II Plus). We investigated theperformance of BD SST™ II Plus tubes for tumor marker tests using BD Serum Glass tubes as controls. Equivalence between the BD SST™ II Plus and BD Serum Glass tubes was demonstrated for all analytes at initial time. Also, all analytes remained stable when stored in BD SST™ II Plus tubes up to 72 hr. Concentration of neuron‐specific enolase tended to increase with preservation time up to 72 hr in BD Serum Glass tubes. We conclude that BD SST™ II Plus was suitable for collection of blood and storage of serum for tumor marker tests. J. Clin. Lab. Anal. 24:418–421, 2010. © 2010 Wiley‐Liss, Inc.
Facile routes for the preparation of 3,4‐disubstituted 1,3‐oxazolidines and 1,2,5‐trisubstituted imidazolidin‐4‐ones
Authors: Catalano Alessia , Carocci Alessia , Lentini Giovanni , Di Mola Antonia , Bruno Claudio , Franchini Carlo 
Published: November 2010

AbstractFacile, alternative synthetic routes to 6, (R)‐6, and (S)‐6‐3‐benzyl‐N‐(2,6‐dimethylphenyl)‐1,3‐oxazolidine‐4‐carboxamides (6), a chiral oxazolidine derivative of tocainide, are reported. The synthetic routes described herein also afforded 11‐, (R)‐11‐, and 12, which present the imidazolidin‐4‐one core and belong to a class of compounds interesting for their biological activities. All the final compounds and intermediates were fully characterized. Enantiomeric excesses of homochiral 6 and 11 were determined by capillary electrophoresis analysis using 2‐hydroxypropyl‐β‐cyclodextrin or highly sulfated γ‐cyclodextrin as chiral selectors. J. Heterocyclic Chem., (2010)
Optimization of genetic algorithm for reconstruction of cross‐phase modulation frequency‐resolved optical gating data
Authors: Vrany Boleslav , Honzatko Pavel , Kanka Jiri 
Published: November 2010

AbstractWe implemented a frequency‐resolved optical gating (FROG) measurement method using cross‐phase modulation in a highly birefringent microstructure optical fiber. The standard FROG data reconstruction algorithm of generalized projection failed for this setup and we successfully used the genetic algorithm (GA) instead which is less prone to get stuck to local minima. GA has several parameters that must be set up to suitable values. We describe the experimental setup and the algorithm and present our findings on what GA parameters are crucial and what are their optimal values. A single run of the algorithm takes 7 min on contemporary CPUs, making the algorithm suitable for routine laboratory work. Copyright © 2010 John Wiley & Sons, Ltd.
The method of lines for the analysis of composite‐metal lossy microstrip lines
Authors: Gao Bo , Tong Ling , Gong Xun 
Published: November 2010

AbstractA full‐wave analysis of the composite‐metal microstrip lines is presented. The effects of the composite metal to the loss of the microstrip lines are accurately investigated, which are often avoided by other methods. The metal layers of the microstrip lines are considered as lossy inhomogeneous layers. The effects of the thickness of the Au layer and Ti layer are given, respectively. And the reasons that lead to the differences between them are discussed for the first time by analyzing the longitudinal electric field on the strip. The results are compared with other published data. Copyright © 2010 John Wiley & Sons, Ltd.
Trilinear multiplicative modelling of thin layer chromatography retention as a function of solute, organic modifier and its concentration
Authors: Komsta Łukasz , Radoń Małgorzata , Radkiewicz Beata , Skibiński Robert 
Published: November 2010

AbstractWe have recently shown in a preliminary study, that the retention in TLC, expressed as RF values, can be modelled in multiplicative trilinear way (solute×mobile phase×stationary phase), explaining almost all variance. Such possibility did not occur in the case of k or RM values. This paper is a continuation of investigation about this phenomenon. We perform here a trilinear decomposition of two datasets (normal‐phase and RP) of 35 model compounds organized differently as in the previous study: solute × modifier × concentration. The resulted models explain 96.8% of whole variance on silica and 90.33% of RP18 variance. No strict correlation with any particular molecular descriptor was found, although the mean electrotopological state index (Ms) was fairly correlated with RP contributions. The results confirm definitively a general possibility to model TLC retention in a multiplicative manner and inspire for further research in this field.
Sinonasal abnormalities in patients with graves' orbitopathy
Authors: Soler Zachary M. , Platt Michael P. , Leung Man‐kit , Mong Sandy , Metson Ralph 
Published: November 2010

AbstractObjectives:To compare radiographic sinus mucosal thickening in patients with Graves' orbitopathy versus controls and determine whether target autoantigens thought to underlie systemic manifestations of Graves' disease are present within paranasal sinus mucosa.Study Design:Case‐control study.Methods:Sinus computed tomography (CT) scans from patients with Graves' orbitopathy scheduled for orbital decompression surgery (n = 50) were compared with maxillofacial CT scans from control patients (n = 50). Scans were rated for sinus mucosal disease by two independent reviewers using the Lund‐MacKay and Harvard staging systems. Gene expression for thyrotropin receptor and type 1 insulin‐like growth factor receptor (IGF‐1R) was assessed in ethmoid mucosal samples using real‐time quantitative polymerase chain reaction (RT‐qPCR) and immunohistochemistry (IHC).Results:Increased sinus mucosal thickening was found in patients with Graves' orbitopathy compared with controls for both the Lund‐MacKay (6.2 vs. 2.7; P < .01) and Harvard (2.1 vs. 1.3; P < .01) systems. Compared with control scans, the Graves' orbitopathy group exhibited greater mucosal thickening in the ethmoid, frontal, and sphenoid sinuses (P < .05 for all). RT‐qPCR and IHC demonstrated gene expression for both the thyrotropin receptor and the IGF‐1R in ethmoid mucosa.Conclusions:Patients with Graves' orbitopathy who present for orbital decompression exhibit a higher prevalence of sinus mucosal thickening on CT scan. These findings may be explained by circulating autoantibodies to target antigens, thyrotropin receptor and IGF‐1R, associated with Graves' disease and found to be expressed in sinus mucosa. Laryngoscope, 2011
Rotigotine effects on early morning motor function and sleep in Parkinson's disease: A double‐blind, randomized, placebo‐controlled study (RECOVER)
Authors: Trenkwalder Claudia , Kies Bryan , Rudzinska Monika , Fine Jennifer , Nikl Janos , Honczarenko Krystyna , Dioszeghy Peter , Hill Dennis , Anderson Tim , Myllyla Vilho , Kassubek Jan , Steiger Malcolm , Zucconi Marco , Tolosa Eduardo , Poewe Werner , Surmann Erwin , Whitesides John , Boroojerdi Babak , Chaudhuri Kallol Ray , the RECOVER Study Group  
Published: November 2010

AbstractIn a multinational, double‐blind, placebo‐controlled trial (NCT00474058), 287 subjects with Parkinson's disease (PD) and unsatisfactory early‐morning motor symptom control were randomized 2:1 to receive rotigotine (2–16 mg/24 hr [n = 190]) or placebo (n = 97). Treatment was titrated to optimal dose over 1–8 weeks with subsequent dose maintenance for 4 weeks. Early‐morning motor function and nocturnal sleep disturbance were assessed as coprimary efficacy endpoints using the Unified Parkinson's Disease Rating Scale (UPDRS) Part III (Motor Examination) measured in the early morning prior to any medication intake and the modified Parkinson's Disease Sleep Scale (PDSS‐2) (mean change from baseline to end of maintenance [EOM], last observation carried forward). At EOM, mean UPDRS Part III score had decreased by −7.0 points with rotigotine (from a baseline of 29.6 [standard deviation (SD) 12.3] and by −3.9 points with placebo (baseline 32.0 [13.3]). Mean PDSS‐2 total score had decreased by −5.9 points with rotigotine (from a baseline of 19.3 [SD 9.3]) and by −1.9 points with placebo (baseline 20.5 [10.4]). This represented a significantly greater improvement with rotigotine compared with placebo on both the UPDRS Part III (treatment difference: −3.55 [95% confidence interval (CI) −5.37, −1.73]; P = 0.0002) and PDSS‐2 (treatment difference: −4.26 [95% CI −6.08, −2.45]; P < 0.0001). The most frequently reported adverse events were nausea (placebo, 9%; rotigotine, 21%), application site reactions (placebo, 4%; rotigotine, 15%), and dizziness (placebo, 6%; rotigotine 10%). Twenty‐four‐hour transdermal delivery of rotigotine to PD patients with early‐morning motor dysfunction resulted in significant benefits in control of both motor function and nocturnal sleep disturbances. © 2010 Movement Disorder Society
Bioactivity of AAV2‐neurturin gene therapy (CERE‐120): Differences between Parkinson's disease and nonhuman primate brains
Authors: Bartus Raymond T. , Herzog Christopher D. , Chu Yaping , Wilson Alistair , Brown Lamar , Siffert Joao , Johnson Eugene M. , Olanow C. Warren , Mufson Elliott J. , Kordower Jeffrey H. 
Published: November 2010

AbstractBackground: AAV2‐neurturin (CERE‐120) is designed to deliver the neurotrophic‐factor, neurturin, to the striatum to restore and protect degenerating nigrostriatal neurons in Parkinson's disease (PD). A common hypothesis is that following expression in the striatum, neurotrophic‐factors like neurturin (NRTN) will be transported from degenerating terminals to their cell bodies in the substantia nigra pars compacta (SNc). Methods: We tested this concept using immunohistochemistry, comparing the bioactivity of AAV2‐neurturin in brains of PD patients versus those of nonhuman primates similarly treated. Results: NRTN‐immunostaining in the targeted striatum was seen in all PD cases (mean putaminal coverage: ∼15% by volume); comparable expression was observed in young, aged, and parkinsonian monkeys. In the SNc cell bodies, however, only rare evidence of neurturin was seen in PD, while ample evidence of intense nigral‐NRTN was observed in all monkeys. NRTN‐expression was associated with occasional, sparse TH‐induction in the striatum of PD, but nothing apparent in the SNc. In primates, NRTN produced robust TH‐induction throughout the nigrostriatal neurons. Discussion: These data provide the first evidence that gene therapy can increase expression of a neurotrophic‐factor deep in the PD brain and that clear but modest enhancement of degenerating neurons can be induced. They also provide important insight regarding deficiencies in the status of nigrostriatal neurons in advanced PD, suggesting that serious axon‐transport deficits reduced the bioactivity of AAV2‐NRTN by limiting the protein exposed to the cell body. Thus, future efforts using neurotrophic‐factors to treat neurodegenerative diseases will need to target both the terminal fields and the cell bodies of degenerating neurons to assure maximal benefit is achieved. © 2010 Movement Disorder Society
Covalent VEGF protein immobilization on resorbable polymeric surfaces
Authors: Edlund U. , Sauter T. , Albertsson A.‐C. 
Published: November 2010

AbstractVascular endothelial growth factor (VEGF) type protein, a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L‐lactic acid) (PLLA) and poly(ε‐caprolactone) through a three‐step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non‐destructive and solvent‐free vapor‐phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride/N‐hydroxysuccinimide chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with X‐ray photoelectron spectroscopy, attenuated total reflectance–Fourier transform infrared spectrometry, UV–VIS, scanning electron microscopy, and enzyme‐linked immunosorbent assay. Copyright © 2010 John Wiley & Sons, Ltd.
Pro‐apoptotic peptide‐polymer conjugates to induce mitochondrial‐dependent cell death
Authors: Adar Lily , Shamay Yosi , Journo Gal , David Ayelet 
Published: November 2010

AbstractThe treatment of cancer using pro‐apoptotic peptides requires efficient intracellular delivery to trigger mitochondrial disruption. When the widely used non‐toxic heptapeptide dimer D(KLAKLAK)2 is coupled with tumor targeting peptides that allow receptor‐mediated internalization, it disrupts negatively charged membranes and induces mitochondrial‐dependent apoptosis. Here, we describe the design, synthesis, and characterization of novel water‐soluble HPMA copolymers bearing a multivalent display of NGR motifs that can target the pro‐apoptotic drug D(KLAKLAK)2 to CD13‐overexpressing cells. D(KLAKLAK)2 was attached to the polymeric backbone through an acid‐sensitive hydrazone linker to facilitate intracellular drug release. The ability of an array of NGR motifs, with different structural conformations, to target several CD13 receptors and thus enhance the binding avidity and the pro‐apoptotic activity of D(KLAKLAK)2 in human cell lines was tested. HPMA copolymer conjugates bearing the dimeric (GNGRG)2 and cyclic (CNGRC) NGR motifs exhibited preferential binding to CD13‐overexpressing cells relative to the linear (GNGRG) targeting peptide. The binding of D(KLAKLAK)2 to the polymer facilitated rapid internalization and translocation into endosomal/lysosomal compartments. The polymer‐bound D(KLAKLAK)2 markedly increased the cytotoxicity and the pro‐apoptotic activity of the drug, relative to free D(KLAKLAK)2 and the targeted pro‐apoptotic peptide (GNGRGG‐D(KLAKLAK)2) in CD13(+) cells. This research is the first attempt to apply polymer‐conjugates with NGR motifs as targeting ligand to deliver the pro‐apoptotic D(KLAKLAK)2 sequence into CD13‐overexpressing cells and thereby to promote the death of cancer cells. Copyright © 2010 John Wiley & Sons, Ltd.
Activated oncogenic pathways and therapeutic targets in extranodal nasal‐type NK/T cell lymphoma revealed by gene expression profiling
Authors: Ng Siok‐Bian , Selvarajan Viknesvaran , Huang Gaofeng , Zhou Jianbiao , Feldman Andrew L , Law Mark , Kwong Yok‐Lam , Shimizu Norio , Kagami Yoshitoyo , Aozasa Katsuyuki , Salto‐Tellez Manuel , Chng Wee‐Joo 
Published: November 2010

AbstractWe performed comprehensive genome‐wide gene expression profiling (GEP) of extranodal nasal‐type natural killer/T‐cell lymphoma (NKTL) using formalin‐fixed, paraffin‐embedded tissue (n = 9) and NK cell lines (n = 5) in comparison with normal NK cells, with the objective of understanding the oncogenic pathways involved in the pathogenesis of NKTL and to identify potential therapeutic targets. Pathway and network analysis of genes differentially expressed between NKTL and normal NK cells revealed significant enrichment for cell cycle‐related genes and pathways, such as PLK1, CDK1, and Aurora‐A. Furthermore, our results demonstrated a pro‐proliferative and anti‐apoptotic phenotype in NKTL characterized by activation of Myc and nuclear factor kappa B (NF‐κB), and deregulation of p53. In corroboration with GEP findings, a significant percentage of NKTLs (n = 33) overexpressed c‐Myc (45.4%), p53 (87.9%), and NF‐κB p50 (67.7%) on immunohistochemistry using a tissue microarray containing 33 NKTL samples. Notably, overexpression of survivin was observed in 97% of cases. Based on our findings, we propose a model of NKTL pathogenesis where deregulation of p53 together with activation of Myc and NF‐κB, possibly driven by EBV LMP‐1, results in the cumulative up‐regulation of survivin. Down‐regulation of survivin with Terameprocol (EM‐1421, a survivin inhibitor) results in reduced cell viability and increased apoptosis in tumour cells, suggesting that targeting survivin may be a potential novel therapeutic strategy in NKTL. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Strategies to improve cyanine dye multi layer organic solar cells
Authors: Hany Roland , Fan Bin , de Castro Fernando Araujo , Heier Jakob , Kylberg William , Nüesch Frank 
Published: November 2010

AbstractWe report the status and strategies to improve the performance of cyanine dye/C60 bilayer heterojunction solar cells. Cyanines are characterized by convenience of synthesis and tunable light absorption. They have high light extinction coefficients, which offer the possibility to go around the constraints of small exciton diffusion length, and ultrathin films can be applied to fabricate multi layer organic solar cells. We optimized the cell performance by oxidative doping (with O2/water/light, or NOBF4) of the cyanine layer. Doping increased the film conductivity and induced a steep rise in the short‐circuit current and the open‐circuit voltage. The external quantum efficiency approached 80% at maximum, meaning that the full cyanine layer of 30 nm thickness was active in charge generation. We argue that cyanine aggregates may be responsible for efficient charge transport over larger distances to the heterointerface. We also describe simple processes to match the energy to the low‐lying hole‐transporting cyanine level by using a thin organic salt layer or doped polyaniline as anode buffer layer. Thereby, hole injection was facilitated and good‐performing devices (power conversion efficiency η = 3%) with a high fill factor (FF ∼60%) were fabricated. Cyanine dyes are accompanied by mobile counter ions. These ions can diffuse into adjacent layers and build up important space charge that alters the energy levels at heterojunctions. The control of the ionic space charge allows to control the flow of electronic charge carriers and to implement new functionalities unique to organic opto‐electronic devices. Copyright © 2010 John Wiley & Sons, Ltd.
Protein expression profiles distinguish between experimental invasive pulmonary aspergillosis and Pseudomonas pneumonia
Authors: Gonzales Denise A. , Torre Carlos De , Wang Honghui , Devor Christopher B. , Munson Peter J. , Ying Sai‐Xia , Kern Steven J. , Petraitiene Ruta , Levens David L. , Walsh Thomas J. , Suffredini Anthony F. 
Published: November 2010

AbstractWe hypothesized that invasive pulmonary aspergillosis (IPA) may generate a distinctive proteomic signature in plasma and bronchoalveolar lavage (BAL). Proteins in plasma and BAL from two neutropenic rabbit models of IPA and Pseudomonas pneumonia were analyzed by SELDI‐TOF MS. Hierarchical clustering analysis of plasma time course spectra demonstrated two clusters of peaks that were differentially regulated between IPA and Pseudomonas pneumonia (57 and 34 peaks, respectively, p<0.001). PCA of plasma proteins demonstrated a time‐dependent separation of the two infections. A random forest analysis that ranked the top 30 spectral points distinguished between late Aspergillus and Pseudomonas pneumonias with 100% sensitivity and specificity. Based on spectral data analysis, three proteins were identified using SDS‐PAGE and LC/MS and quantified using reverse phase arrays. Differences in the temporal sequence of plasma haptoglobin (p<0.001), apolipoprotein A1 (p<0.001) and transthyretin (p<0.038) were observed between IPA and Pseudomonas pneumonia, as was C‐reactive protein (p<0.001). In summary, proteomic analysis of plasma and BAL proteins of experimental Aspergillus and Pseudomonas pneumonias demonstrates unique protein profiles with principal components and spectral regions that are shared in early infection and diverge at later stages of infection. Haptoglobin, apolipoprotein A1, transthyretin, and C‐reactive protein are differentially expressed in these infections suggesting important contributions to host defense against IPA.
Protected versus unprotected dextran macroinitiators for ATRP synthesis of Dex‐g‐PMMA
Authors: Dupayage Ludovic , Nouvel Cécile , Six Jean‐Luc 
Published: November 2010

AbstractThe synthesis of amphiphilic dextran‐g‐poly(methyl methacrylate) glycopolymers (Dex‐g‐PMMA) is studied using “grafting from” concept and atom transfer radical polymerization. Two strategies have been examined to control the macromolecular parameters of such glycopolymers. One is involving four steps including a protection/deprotection approach and the second one only two steps. The introduction of initiators group onto a protected acetylated dextran (and directly onto dextran) was achieved resulting in protected DexAcBr (and in unprotected DexBr). These two types of polysaccharidic macroinitiators differ in term of solubility (hydrophilic DexBr vs. hydrophobic DexAcBr) and of position of the initiators groups on the glucosidic units (which are the sites of the future grafts). When evaluated as macroinitiators for ATRP of MMA, control was achieved in both cases but DexBr gave much faster polymerization and lower average grafting efficiency compared with DexAcBr or model initiator. Advantages and drawbacks of both pathways have finally been discussed. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010