bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting lymph node metastasis in papillary thyroid carcinoma: radiomics using two types of ultrasound elastography
Authors: Zhang Xian-Ya , Zhang Di , Zhou Wang , Wang Zhi-Yuan , Zhang Chao-Xue , Li Jin , Wang Liang , Cui Xin-Wu 
Published: February 2025

Abstract

Background
To develop a model based on intra- and peritumoral radiomics features derived from B-mode ultrasound (BMUS), strain elastography (SE), and shear wave elastography (SWE) for cervical lymph node metastasis (LNM) prediction in papillary thyroid cancer (PTC) and to determine the optimal peritumoral size.

Methods
PTC Patients were enrolled from two medical centers. Radiomics features were extracted from intratumoral and four peritumoral regions with widths of 0.5–2.0 mm on tri-modality ultrasound (US) images. Boruta algorithm and XGBoost classifier were used for features selection and radiomics signature (RS) construction, respectively. A hybrid model combining the optimal RS with the highest AUC and clinical characteristics as well as a clinical model were built via multivariate logistic regression analysis. The performance of the established models was evaluated by discrimination, calibration, and clinical utility. DeLong’s test was used for performance comparison. The diagnostic augmentation of two radiologists with hybrid model’s assistance was also evaluated.

Results
A total of 660 patients (mean age, 41 years ± 12 [SD]; 506 women) were divided into training, internal test and external test cohorts. The multi-modality RS1.0 mm yielded the optimal AUCs of 0.862, 0.798 and 0.789 across the three cohorts, outperforming other single-modality RSs and intratumoral RS. The AUCs of the hybrid model integrating multi-modality RS1.0 mm, age, gender, tumor size and microcalcification were 0.883, 0.873 and 0.841, respectively, which were significantly superior to other RSs and clinical model (all p < 0.05). The hybrid model assisted to significantly improve the sensitivities of junior and senior radiologists by 19.7% and 18.3%, respectively (all p < 0.05).

Conclusions
The intra-peritumoral radiomics model based on tri-modality US imaging holds promise for improving risk stratification and guiding treatment strategies in PTC.

Trial registration
Retrospectively registered.
Comparative oncology in action: vignettes on immunotherapy development
Authors: LeBlanc Amy , Mazcko Christina N. , Mason Nicola J. , Chambers M. Renee , Brockington David M. , Pluhar G. Elizabeth , Naik Shruthi 
Published: February 2025

Abstract
Immunotherapeutic approaches to cancer treatment have gained significant traction in recent years, due in large part to the success of immune checkpoint inhibitors and T cell-based therapies. Comparative oncology is the study of naturally-occurring cancer in companion (pet) animals, mainly dogs, and is a powerful tool in cancer research and drug development. Given their intact, educated immune systems and natural co-evolution of tumor, microenvironment and stromal components, tumor-bearing pet dogs are an attractive species in which to explore these cellular interactions and test novel therapeutic approaches. Moreover, similarities between the canine and human immune systems support assessment of a wide variety of approaches, including antagonistic or agonistic antibodies directed at specific cellular targets, tumor vaccines, cell-based therapies, and combinations of these with conventional cancer treatments such as chemotherapy and radiotherapy. This manuscript provides specific examples of how canine immunotherapeutic studies informed an approach destined for human use, with an emphasis on study design, correlative immune assay development and application, and definition of biologic effect.
Scale Control in Geothermal Wells—What Are the Options for Effective and Economic Scale Management?
Authors: Jarrahian Khosro , Mackay Eric , Singleton Michael , Mohammadi Shabnam , Heath Stephen Mark , Pessu Frederick 
Published: February 2025

Summary
The drive towards net zero by 2050 has led to the development of alternative energy sources, with a focus on geothermal energy projects. Geothermal brines often contain dissolved minerals and gases that cause scale deposition in wells and plant equipment, leading to decreased efficiency in thermal energy production and reduced flow capacity in injection wells. Geothermal plant conditions and brine chemistry of each geothermal plant vary greatly, making the selection of a suitable scale management process crucial for sustainable energy production. Prevention through chemical inhibition or removal is preferred over costly mechanical methods, though nonchemical alternatives are attractive for reducing the CO2 footprint.
Chemical treatment options include continuous topside injection, scale squeeze, or batch treatment in injection/production wells. High temperatures and water throughput in geothermal wells challenge chemical scale control and removal. Selected inhibitors/dissolvers must be thermally stable up to 250°C, preferably biodegradable, and effective against calcium carbonate and silica/silicate scales. Geothermal brines contain dissolved CO2 and H2S, making fluids corrosive. Therefore, the interaction between scale and corrosion inhibitors must be considered in any management strategy, possibly requiring modified laboratory test methods, especially for plants with water reinjection.
This paper will review geothermal scale and its mechanisms of formation and management options, including chemical and nonchemical treatments. It also discusses the impact of well completion, high water production, corrosion, CO2 footprint, and the challenges of chemical treatments regarding thermal stability and laboratory testing.
WITHDRAWN: Zinc deficiency triggers ribosome remodeling to reverse biofilm azole resistance in Candida albicans
Authors: Wang Yingzhe , Ye Shigan , Deng Yuan , Huang Yingting , Zhu Xiaoliang 
Published: February 2025

Abstract

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
One-step drug transport across two membranes of Gram-negative bacteria by an MFS tripartite assembly
Authors: Luisi Ben , Du Dijun , Chao Yanjie , Zhong Zhaojun , Maimaiti Tuerxunjiang , Jackson Matthew , Dong Rui , Gao Xueyan , Guo Jinliang , Li Shangrong , Shang Wenyu , Liu Huajun , Jiang Hongnian , Zhang Shuo , Zhao Huimin , Ouyang Qing 
Published: February 2025

Abstract

Transport of proteins and small molecules across cellular membrane is crucial for bacterial interaction with the environment and survival against antibiotics. In Gram-negative bacteria that possess two layers of membranes, specialized macromolecular machines are required to transport substrates across the envelope, often via an indirect stepwise procedure. The major facilitator superfamily (MFS)-type tripartite efflux pumps and type I secretion systems likely employ a direct one-step transport approach, but the structural mechanisms remain elusive. A representative MFS-type tripartite efflux pump, EmrAB-TolC, mediates resistance to multiple antimicrobial drugs through proton-coupled EmrB, a member of the DHA2 transporter family. Here, we report the high-resolution (3.13 Å) structure of the EmrAB-TolC pump, revealing a unique, asymmetric architecture emerging from the assembly of TolC:EmrA:EmrB with a ratio of 3:6:1. This structure reveals two critical subdomains AssA and AssB that are essential for the pump assembly. Key residues involved in drug recognition, proton translocation and coupling are identified and corroborated by mutagenesis and antibiotic sensitivity assays. The delineation of the complete translocation pathway unravels the one-step drug transport mechanism of the MFS pump across the entire envelope of Gram-negative bacteria.
Detrimental Impacts of High-Fat Diet on Colorectal Cancer Development: An Update on Molecular Mechanisms
Authors: Ponnusamy Bhuvaneswari , Rajagopal Ponnulakhmi , Sirasanagandla Srinivasa Rao , Ker Woon Choy , Veeraraghavan Vishnu Priya , Jayaraman Selvaraj 
Published: February 2025

Abstract:
Lifestyle modifications lead to a number of pathological conditions of which colorectal
cancer (CRC) has a greater consideration as the number of cases is constantly increasing. As the
lifestyle changes, there is a tremendous change in the food habits of people worldwide. Consumption
of fatty foods in excess amounts is a growing trend among people. This shift towards a high--
fat diet (HFD) tends to increase the pathological burden on society. Recent investigations have reported
the association of HFD consumption with CRC; it is very surprising that HFD can promote
carcinogenesis, of which CRC is one of its kind. It has become evident that one modifiable risk
factor of CRC is the diet we consume. Although fats are an important macromolecule in the normal
functioning of the body, it is necessary to be aware of which type of fats we consume in a
greater proportion. Consumption of saturated fats and ω-6 polyunsaturated fatty acids in a higher
proportion with a lower proportion of polyunsaturated fatty acids and monounsaturated fatty acids
can result in pathological conditions. In this review, we highlight the possible mechanistic pathways
and biomarkers underlying HFD-induced CRC. HFD increases the fatty acid levels in the circulation
and deposition of fat in various parts, which can result in a chronic inflammatory condition
that gradually leads to cancer formation. Thus, it is essential to create awareness among society
about the growing incidence of CRC and its association with HFD.
Bioinformatics Analysis of Key Genes Associated with Resistance to the Combination of Bevacizumab and Pemetrexed Chemotherapy in Non-small Cell Lung Cancer
Authors: Hu Chenling , Xu Shenjie , Chen Siwen , Sun Qian , Pan Xudong 
Published: February 2025

Objective:
This study aimed to identify key genes linked to resistance to a combination
treatment regimen of bevacizumab and pemetrexed in non-small cell lung cancer (NSCLC)
through bioinformatics analysis and analysis of their associated pathways.

Methods:
Expression data from the Gene Expression Omnibus (GEO) database (GSE154286)
were analyzed. The differentially expressed genes (DEGs) between tissues sensitive and resistant
to combined bevacizumab and pemetrexed treatment in NSCLC were identified. Gene
Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment was investigated,
and protein-protein interaction (PPI) networks, as well as transcription factor (TFs)-
DEGs-miRNA networks, were created using the STRING tool. Key genes were identified with
the help of the MCODE plugin. Additionally, gene set enrichment analysis (GSEA) was utilized
to identify pathways linked to the key genes. A retrospective analysis was conducted on clinical
data from 80 NSCLC patients. Patients were categorized into drug-resistant and non-resistant
groups based on RECIST1.1 criteria. The expression of the key gene TNFSF4 was analyzed
using quantitative real-time PCR (qRT-PCR).

Results:
In the GSE154286 dataset, 35 downregulated DEGs were discovered. KEGG pathway
enrichment analysis revealed that these DEGs were primarily associated with immunity and
inflammation-related pathways. The PPI network construction highlighted a significant module
and led to the identification of 8 candidate genes: TNFRSF18, TNFSF4, LGALS9, FAS, LAG3,
CD86, CD80, and FOXP3. The TFs-DEGs-miRNA network analysis pinpointed TNFSF4 as a
key gene, potentially regulated by 7 transcription factors and interacting with 9 miRNAs. GSEA
analysis suggested that TNFSF4 may influence NSCLC’s pathological processes through involvement
in pathways involved in chemokine, JAK/STAT, NOD-like receptor, T cell receptor,
toll-like receptor, and PPAR signaling. qRT-PCR detection displayed significantly lower expression
of TNFSF4 in the peripheral blood of the patients in the resistant group relative to the
non-resistant group (p < 0.0001). Logistic regression analysis showed that low TNFSF4 levels
were independently linked to a raised risk of resistance to bevacizumab combined with
pemetrexed therapy in lung adenocarcinoma patients.

Conclusion:
The identification of key genes, such as TNFSF4, and resistance-related signaling
pathways through bioinformatics analysis offers valuable insights into potential mechanisms of
chemotherapy resistance in NSCLC when treated with the combination of bevacizumab and
pemetrexed. These findings provide a theoretical foundation for advancing clinical research on
diagnosis and treatment.
Prediction of the Prognosis and Treatment Responses Based on the Characteristics of Disulfidptosis-Related Genes in Patients with
Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
Authors: Kang Min , Jiang Sha , Chen Huihui , Xu Youhua , Mo Hui 
Published: February 2025

Background:
Disulfidptosis is a new type of regulatory cell death (RCD), but the pathophysiological
functions and mechanisms of DRGs in CESC remain to be examined.

Aims:
This study explored the mutation status of disulfidptosis-related genes (DRGs) in cervical
squamous cell carcinoma and endocervical adenocarcinoma (CESC).

Objective:
After analyzing the mutation profiles of DRGs in CESC, this study established a prognostic
model for CESC and also explored the differences in immune infiltration (accumulation of
immune system cells in tissues or organs), related enriched pathways, and drug sensitivity between
high-risk and low-risk CESC groups.

Methods:
The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) were accessed
to source related data. The mutation profiles of DRGs in CESC were analyzed using Mutect2
software, and disulfidptosis scores were calculated by ssGSEA. WGCNA was performed to
identify modular genes, which were further filtered and used to formulate a risk model by applying
the survival and glmnet packages. Low- and high-risk groups of CESC patients were classified
using the survminer package. GSEA was performed to conduct pathway analysis, and immune infiltration
was assessed using the MCPcounter package, ESTIMATE, and TIMER algorithms. Finally,
immunotherapy response and drug sensitivity were analyzed using the TIDE method and
the pRRophetic package, respectively.

Results:
Except for NDUFA11, ARL6IP5, EPM2AIP1, GBE1, RBM38, ULK4, and ZBTB47 were
found to be the DRGs significantly mutated in CESC. The six genes were integrated to develop a
RiskScore model with a relatively high Area Under the Curve (AUC) value. Significant differences
between the two risk groups were determined, indicating that the model was highly reliable.
Notably, the low-risk group was enriched in energy metabolism-correlated pathways, while the
high-risk group was primarily enriched in immune-correlated pathways. The high-risk group
showed higher immune cell activity, higher TIDE score, and more B cells than the low-risk group.
Drug sensitivity study revealed that the high-risk group was more sensitive to chemotherapy
drugs.

Conclusion:
This study provides novel insights into CESC prognosis, immunotherapy, and drug
development, contributing to the clinical treatment for CESC.
Cutaneous Atrophy Following Corticosteroid Injections for Tendonitis: Report of Two Cases
Authors: Colwell Rebecca , Gullickson Mitchell , Cutlan Jonathan , Stratman Erik 
Published: February 2025

Abstract
Cutaneous atrophy resulting from corticosteroid injections for musculoskeletal indications is an underrecognized adverse effect among orthopedists and dermatologists. We present two cases of cutaneous atrophy following corticosteroid injections for wrist tendonitis. Patients presenting with cutaneous atrophy following orthopedic corticosteroid injections may be misdiagnosed with linear morphea, atrophoderma, or vascular disorders and receive unnecessary workups and delays in appropriate management. Dermatologists play an essential role in the evaluation of these patients.
Impact of BRCA Genes Mutation on Gynecological Disorders: Beyond Oncological Risk
Authors: Max Lourdes Judith Medeiros , Barros Eveline Rodrigues da Silva , Mascarenhas Luiz Eduardo Moura , Noleto Gabriel Guimarães , Costa Marília Mendes Confort , Biato Mariana de Freitas Teixeira , Costa Washington Lucas Alves da , Pereira Taiana de Andrade 
Published: February 2025

Objectives: This systematic review aims to assess the impact of mutations in the BRCA1 and BRCA2 genes on gynecological disorders beyond oncological risk, focusing on fertility and reproductive health.

Theoretical Framework: While mutations in the BRCA1 and BRCA2 genes are widely recognized for their association with an increased risk of breast and ovarian cancer, recent studies suggest that these mutations also play a crucial role in ovarian reserve, menopausal age, and endocrine function.

Method: The research followed PRISMA guidelines and included publications from 2010 to 2024 retrieved from PubMed. Six studies were selected, demonstrating the direct link between BRCA1 mutations and reduced ovarian reserve and premature ovarian aging.

Results and Discussion: The findings indicate that BRCA1 mutations are associated with reduced ovarian reserve and premature ovarian aging, factors that may compromise fertility. These results highlight the need for proactive strategies such as reproductive monitoring and fertility preservation for women carrying these mutations.

Implications of the Research: This review expands the understanding of the impacts of BRCA mutations on gynecological disorders, suggesting that women with these mutations should undergo comprehensive follow-up, not only for cancer prevention but also for reproductive care.

Originality/Value: This study provides significant contributions to gynecological practice, emphasizing the importance of an integrated approach that considers both oncological risks and reproductive needs for women with BRCA mutations.
Situation of Employment Creation for Disabled Workers in Vietnam After the COVID-19 Pandemic
Authors: Thu Hang Nguyen Thi , Anh Thinh Cao , Thi Tuyet Do 
Published: February 2025

Objective: The general objective of this study is to assess the current situation of employment for disabled workers in Vietnam. To achieve this objective, the following specific objectives have been set: clarifying the theoretical and legal basis of disabled workers and employment of disabled workers; assessing the activities that Vietnam has done to solve employment for disabled workers.

Theoretical Framework: The Universal Declaration of Human Rights of the United Nations General Assembly (1948): “Everyone has the right to work, to free choice of employment, to just and favourable conditions of work and to protection against unemployment”. People with disabilities are no exception. In 2019, Vietnam joined Convention 159 of the International Labor Organization - ILO. This is an important step in solving employment for people with disabilities, strongly affirming Vietnam's commitment to ensuring and creating favorable conditions for workers with disabilities to avoid discrimination.

Method: The method applied for this study includes analyzing the management and implementation of employment solutions, by synthesizing and processing data on the employment situation for disabled workers in Vietnam to draw conclusions on this issue.

Results and Discussion: The results show that after being severely affected by the Covid 19 pandemic, many workers have lost their jobs or have had difficulty maintaining their jobs. For workers with disabilities, this difficulty is even more profound. Currently, Vietnam has about 6.2 million people with disabilities and the problem of creating jobs for workers with disabilities not only improves the lives of people with disabilities but also helps reduce the burden on the state budget in subsidizing and supporting people with disabilities.

Research Implications: The theoretical and practical significance of this study will be discussed, providing insights into solving the employment problem in general and employment for disabled workers in particular in Vietnam. While implementing policies, 100% of disabled workers are eligible for social security policies, but long-term solutions for disabled workers have not been widely implemented. Training and job creation models for disabled workers have not been effective and replicated. Synchronizing solutions will create an environment for sustainable and long-term employment for disabled workers in Vietnam.

Originality/Value: This study contributes to the literature on employment for disabled workers in Vietnam. The synchronous implementation of policies and incentives for the private sector will help to solve this problem in a long-term and sustainable manner.
First aid: issues of preparedness and training of the population in cardiopulmonary resuscitation
Authors: Stanishevsky A. L. 
Published: February 2025

One of the reasons for the low survival rate of victims with out-of-hospital cardiac arrest (OHCA) is the low readiness of bystanders to provide first aid (cardiopulmonary resuscitation (CPR) and the use of an automatic external defibrillator (AED).The purpose of the study: to study the degree of readiness of the population for CPR, its determining factors and determine ways to improve the provision of first aid to victims with OHCA.Materials and methods. In January — June 2023, an anonymous survey of workers in the fuel and energy complex of the Republic of Belarus was conducted using the developed questionnaire «Readiness to provide first aid». The study involved 894 respondents without medical education. Nonparametric statistics methods were used to analyze the results.Results. 10.7% of respondents defined their readiness to perform CPR as extremely low; 12.2% — as low; 38.6% — as average; 22.3% — above average; 16.2% — as high. Significant predictors of high readiness of respondents to perform CPR (p <0.001) were: male gender, previous training in CPR skills and participation in performing CPR on a victim in real conditions. The main barriers to performing CPR were fear of causing irreparable harm to the injured person and lack of knowledge and skills in CPR. Conclusion. Reducing mortality in OHCA requires maximum involvement of the population in the process of first aid by means of broad information, formation of proper motivation and emotional-volitional readiness, and increasing the coverage of practical training in first aid skills.