bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isothermal aging treatment and its effect on the mechanical properties of Fe-Cr-Mn high-nitrogen austenitic steel
Authors: Ararat Özge , Erişir Ersoy 
Published: May 2024

Abstract
The high-temperature solution nitriding process is a suitable treatment for producing high-nitrogen steels by the diffusion of nitrogen from the surface to the center of steels under high nitrogen pressure at high temperatures. On the other hand, long-term solution nitriding at high temperatures can cause the formation of coarse-grained austenite. This study focused on the grain size, strength, and elongation properties of Fe-Cr-Mn high-nitrogen austenitic steels by isothermal aging. For this purpose, we produced high-nitrogen steels by three-step phase transformations: (i) high-temperature solution nitriding, (ii) isothermal aging, and (iii) reaustenitization. After solution nitriding, high-nitrogen austenitic steel was treated with isothermal aging to induce austenite decomposition. Supersaturated austenite (ϒ) transformed to less nitrogen-saturated austenite (ϒ′) and discontinuous cellular precipitation (DCP) during the isothermal aging treatment. Subsequently, the decomposed structure was reversed into austenite through reaustenitization. The results suggested that austenite grain refinement and increasing hardness were achieved by nucleating reversed austenite grains after reaustenitization treatment. On the other hand, a noticeable increase in elongation appeared after reaustenitization treatment for 30 min.
All inkjet-printed organic solar cells on 3D objects
Authors: Steinberger Marc , Distler Andreas , Hörber Johannes , Tam Kai Cheong , Brabec Christoph J , Egelhaaf Hans-Joachim 
Published: May 2024

Abstract
Drop-on-demand inkjet printing is a promising and commercially relevant technology for producing organic electronic devices of arbitrary shape on a wide variety of different substrates. In this work we transfer the inkjet printing process of organic photovoltaic devices from 2D to 3D substrates, using a 5-axis robot system equipped with a multi-nozzle inkjet printing unit. We present a ready-to-use 3D printing system for industrial application, using a 5-axis motion system controlled by commercial 3D motion software, combined with a commonly used multi-nozzle inkjet print head controlled by the corresponding printing software. The very first time inkjet-printed solar cells on glass/ITO with power conversion efficiencies (PCEs) of up to 7% are realized on a 3D object with surfaces tilted by angles of up to 60° against the horizontal direction. Undesired ink flow during deposition of the inkjet-printed layers was avoided by proper ink formulation. In order to be able to print organic (opto-)electronic devices also on substrates without sputtered indium tin oxide bottom electrode, the bottom electrode was inkjet-printed from silver nanoparticle (AgNP) ink, resulting in the first all inkjet-printed (i.e. including bottom electrode) solar cell on a 3D object ever with a record PCE of 2.5%. This work paves the way for functionalizing even complex objects, such as cars, mobile phones, or ‘Internet of Things’ applications with inkjet-printed (opto-)electronic devices.
Overlapping top gate electrodes based on low temperature atomic layer deposition for nanoscale ambipolar lateral junctions
Authors: Fuchs Christopher , Fürst Lena , Buhmann Hartmut , Kleinlein Johannes , Molenkamp Laurens W 
Published: May 2024

Abstract
We present overlapping top gate electrodes for the formation of gate defined lateral junctions in semiconducting layers as an alternative to the back gate/top gate combination and to the split gate configuration. The optical lithography microfabrication of the overlapping top gates is based on multiple layers of low-temperature atomic layer deposited hafnium oxide, which acts as a gate dielectric and as a robust insulating layer between two overlapping gate electrodes exhibiting a large dielectric breakdown field of

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. The advantage of overlapping gates over the split gate approach is confirmed in model calculations of the electrostatics of the gate stack. The overlapping gate process is applied to Hall bar devices of mercury telluride in order to study the interaction of different quantum Hall states in the nn′, np, pn and pp′ regime.
Floral structure, histochemistry, and volatile compounds in Bulbophyllum species of the ‘Cirrhopetalum alliance’ (Orchidaceae)
Authors: Davies Kevin L , Stpiczyńska Małgorzata , Ludlow Richard A , Wheaton Louise , Aros Danilo , Hailer Frank , Müller Carsten T , Rogers Hilary J 
Published: May 2024

Abstract
Bulbophyllum is the most species-rich orchid genus. Recent molecular data groups certain of its species into a monophyletic ‘Cirrhopetalum alliance’. Within this alliance are a monophyletic section Cirrhopetaloides and a non-monophyletic section Cirrhopetalum. Floral morphology and scent are partially conserved phylogenetically compared to the molecular data. Here, floral morphology, histochemistry, anatomy, and floral volatile organic compounds (VOCs) of selected Bulbophyllum species are analysed to assess whether there are structural or chemical differences that support separation into the two sections, to update available VOC data from this genus, and to consider evolutionary adaptations in the ‘Cirrhopetalum alliance’. We found that ‘Cirrhopetalum alliance’ flowers differ from some sections of Bulbophyllum in the structure of cells lining the longitudinal groove on the labellum or lip and report, for the first time in Bulbophyllum, a type of atypical oleiferous trichome probably involved in VOC production. We show a clear difference in floral VOC profiles between B. bicolor (sect. Cirrhopetaloides) and four other Bulbophyllum species, three previously assigned to sect. Cirrhopetalum, but VOC profiles do not support separation into the two sections. The lack of congruence between already established molecular phylogeny, floral anatomy, and VOC data suggests that VOC differences are due to recently and independently evolved pollinator specificity. We therefore show that there is no clear separation of these sections based on floral anatomy, histochemistry, or VOC profiles.
Power optimization of photovoltaic modules under varying environmental conditions based on current equalization collaborating with constant voltage control
Authors: Tang DaiBin , Siaw Fei Lu , Thio Tzer Hwai Gilbert 
Published: May 2024

Abstract
The performance of photovoltaic (PV) modules is affected by environmental factors such as irradiance and temperature, which can lead to a decrease in output performance or even damage. This study proposes an improved formula for calculating the real maximum power of PV modules by analysing the influence of irradiance and temperature. A simulation model is developed using PLECS software to simulate the global maximum power of PV modules under different environmental conditions and the results are compared with the calculated real maximum power. A power optimization scheme for PV modules is then proposed based on current equalization and constant voltage control. This scheme employs a single-switch multi-winding forward–flyback converter to equalize the mismatched currents between cell strings, thereby enhancing the output performance. Traditional proportional–integral controllers are utilized to achieve constant voltage control and obtain the real maximum power of PV modules. Simulation models are built in the PLECS simulation platform to evaluate the performance of a global maximum power point tracking scheme based on the traditional perturb-and-observe (TPO) algorithm with current equalization, a segment perturb-and-observe algorithm without current equalization, and the proposed power optimization scheme. The simulation results demonstrate that the proposed constant voltage control has greater efficiency than the TPO algorithm. The proposed scheme achieves a significant improvement in efficiency, with a 27.87% increase compared with the segment perturb-and-observe algorithm without current equalization.
Pachyonychia congenita: pathogenesis of pain and approaches to treatment
Authors: McCarthy Rebecca L , de Brito Marianne , O’Toole Edel A 
Published: May 2024

Abstract
Pachyonychia congenita (PC) is an autosomal dominant genodermatosis characterized by a triad of chronic severe plantar pain, focal palmoplantar keratoderma and hypertrophic nail dystrophy. Plantar pain can be debilitating and have a profound impact on quality of life. Current therapeutic options for pain in PC are limited to lifestyle adjustment and mechanical techniques, with a small subgroup of patients benefiting from oral retinoids. This review investigates the pathogenesis of pain in PC and provides a summary of the current and future therapeutic options.
Non-ST-elevation acute coronary syndromes with previous coronary artery bypass grafting: a meta-analysis of invasive vs. conservative management
Authors: Kelham Matthew , Vyas Rohan , Ramaseshan Rohini , Rathod Krishnaraj , de Winter Robbert J , de Winter Ruben W , Bendz Bjorn , Thiele Holger , Hirlekar Geir , Morici Nuccia , Myat Aung , Michalis Lampros K , Sanchis Juan , Kunadian Vijay , Berry Colin , Mathur Anthony , Jones Daniel A 
Published: May 2024

Abstract

Background and Aims
A routine invasive strategy is recommended in the management of higher risk patients with non-ST-elevation acute coronary syndromes (NSTE-ACSs). However, patients with previous coronary artery bypass graft (CABG) surgery were excluded from key trials that informed these guidelines. Thus, the benefit of a routine invasive strategy is less certain in this specific subgroup.

Methods
A systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted. A comprehensive search was performed of PubMed, EMBASE, Cochrane, and ClinicalTrials.gov. Eligible studies were RCTs of routine invasive vs. a conservative or selective invasive strategy in patients presenting with NSTE-ACS that included patients with previous CABG. Summary data were collected from the authors of each trial if not previously published. Outcomes assessed were all-cause mortality, cardiac mortality, myocardial infarction, and cardiac-related hospitalization. Using a random-effects model, risk ratios (RRs) with 95% confidence intervals (CIs) were calculated.

Results
Summary data were obtained from 11 RCTs, including previously unpublished subgroup outcomes of nine trials, comprising 897 patients with previous CABG (477 routine invasive, 420 conservative/selective invasive) followed up for a weighted mean of 2.0 (range 0.5–10) years. A routine invasive strategy did not reduce all-cause mortality (RR 1.12, 95% CI 0.97–1.29), cardiac mortality (RR 1.05, 95% CI 0.70–1.58), myocardial infarction (RR 0.90, 95% CI 0.65–1.23), or cardiac-related hospitalization (RR 1.05, 95% CI 0.78–1.40).

Conclusions
This is the first meta-analysis assessing the effect of a routine invasive strategy in patients with prior CABG who present with NSTE-ACS. The results confirm the under-representation of this patient group in RCTs of invasive management in NSTE-ACS and suggest that there is no benefit to a routine invasive strategy compared to a conservative approach with regard to major adverse cardiac events. These findings should be validated in an adequately powered RCT.
A cyanobacterial chemotaxis-like system controls phototactic orientation via phosphorylation of two antagonistic response regulators
Authors: Han Yu , Hammerl Jonas , Flemming Felicitas E , Schuergers Nils , Wilde Annegret 
Published: May 2024

Abstract
Photosynthetic cyanobacteria exhibit phototaxis, utilizing type IV pili (T4P) to navigate either toward or away from a light source. The Tax1 system is a chemotaxis-like signal transduction pathway that controls the switch in cell polarity, which is crucial for positive phototaxis in Synechocystis sp. PCC 6803. The system consists of the blue/green light sensor PixJ, which controls the histidine kinase PixL and two CheY-like response regulators, PixG and PixH. However, the molecular mechanism by which Tax1 regulates T4P activity and polarity is poorly understood. Here, we investigated the phosphotransfer between PixL and its cognate response regulators in vitro and analyzed the localization and function of wild-type and phosphorylation-deficient PixG and PixH during phototaxis. We found that both PixG and PixH are phosphorylated by PixL but have different roles in phototaxis regulation. Only phosphorylated PixG interacts with the T4P motor protein PilB1 and localizes to the leading cell pole under directional light, thereby promoting positive phototaxis. In contrast, PixH is a negative regulator of PixG phosphorylation and inhibits positive phototaxis. We also demonstrated that the C-terminal receiver domain of PixL is essential for positive phototaxis, and modulates the kinase activity of PixL. Our findings reveal the molecular basis of positive phototaxis regulation by the Tax1 system and provide insights into the division of labor between PatA-type and CheY-like response regulators in cyanobacterial chemotaxis-like systems. Furthermore, these findings highlight similarities in the regulation of movement direction during twitching motility in phototactic and chemotactic bacteria.
Chloroplast protein StFC-II was manipulated by a Phytophthora effector to enhance host susceptibility
Authors: Xu Meng , Sun Xinyuan , Wu Xinya , Qi Yetong , Li Hongjun , Nie Jiahui , Yang Zhu , Tian Zhendong 
Published: May 2024

Abstract
Oomycete secretes a range of RxLR effectors into host cells to manipulate plant immunity by targeting proteins from several organelles. In this study, we report that chloroplast protein StFC-II is hijacked by a pathogen effector to enhance susceptibility. Phytophthora infestans RxLR effector Pi22922 is activated during the early stages of P. infestans colonization. Stable overexpression of Pi22922 in plants suppresses flg22-triggered reactive oxygen species (ROS) burst and enhances leaf colonization by P. infestans. A potato ferrochelatase 2 (FC-II, a nuclear-encoded chloroplast-targeted protein), a key enzyme for heme biosynthesis in chloroplast, was identified as a target of Pi22922 in the cytoplasm. The pathogenicity of Pi22922 in plants is partially dependent on FC-II. Overexpression of StFC-II decreases resistance of potato and Nicotiana benthamiana against P. infestans, and silencing of NbFC-II in N. benthamiana reduces P. infestans colonization. Overexpression of StFC-II increases heme content and reduces chlorophyll content and photosynthetic efficiency in potato leaves. Moreover, ROS accumulation both in chloroplast and cytoplasm is attenuated and defense-related genes are down-regulated in StFC-II overexpression transgenic potato and N. benthamiana leaves. Pi22922 inhibits E3 ubiquitin ligase StCHIP-mediated StFC-II degradation in the cytoplasm and promotes its accumulation in chloroplasts. In summary, this study characterizes a new mechanism that an oomycete RxLR effector suppresses host defenses by promoting StFC-II accumulation in chloroplasts, thereby compromising the host immunity and promoting susceptibility.
Simulation testing performance of ensemble models when catch data are underreported
Authors: Brooks Elizabeth N , Brodziak Jon K T 
Published: May 2024

Abstract
Ensemble model use in stock assessment is increasing, yet guidance on construction and an evaluation of performance relative to single models is lacking. Ensemble models can characterize structural uncertainty and avoid the conundrum of selecting a “best” assessment model when alternative models explain observed data equally well. Through simulation, we explore the importance of identifying candidate models for both assessment and short-term forecasts and the consequences of different ensemble weighting methods on estimated quantities. Ensemble performance exceeded a single best model only when the set of candidate models spanned the true model configuration. Accuracy and precision depended on the model weighting scheme, and varied between two case studies investigating the impact of catch accuracy. Information theoretic weighting methods performed well in the case study with accurate catch, while equal weighting performed best when catch was underreported. In both cases, equal weighting produced multimodality. Ensuring that an ensemble spans the true state of nature will be challenging, but we observed that a change in sign of Mohn’s rho across candidate models coincided with the true OM being bounded. Further development of protocols to select an objective and balanced set of candidate models, and diagnostics to assess adequacy of candidates are recommended.
Spliced-Leader RNA as a Dynamic Marker for Monitoring Viable Leishmania Parasites During and After Treatment
Authors: Hendrickx Rik , Melkamu Roma , Tadesse Dagimawie , Teferi Tedla , Feijens Pim-Bart , Vleminckx Margot , van Henten Saskia , Alves Fabiana , Shibru Tamiru , van Griensven Johan , Caljon Guy , Pareyn Myrthe 
Published: May 2024

Abstract
Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.
RIL-seq reveals extensive involvement of small RNAs in virulence and capsule regulation in hypervirulent Klebsiella pneumoniae
Authors: Goh Kwok Jian , Altuvia Yael , Argaman Liron , Raz Yair , Bar Amir , Lithgow Trevor , Margalit Hanah , Gan Yunn-Hwen 
Published: May 2024

Abstract
Hypervirulent Klebsiella pneumoniae (hvKp) can infect healthy individuals, in contrast to classical strains that commonly cause nosocomial infections. The recent convergence of hypervirulence with carbapenem-resistance in K. pneumoniae can potentially create ‘superbugs’ that are challenging to treat. Understanding virulence regulation of hvKp is thus critical. Accumulating evidence suggest that posttranscriptional regulation by small RNAs (sRNAs) plays a role in bacterial virulence, but it has hardly been studied in K. pneumoniae. We applied RIL-seq to a prototypical clinical isolate of hvKp to unravel the Hfq-dependent RNA-RNA interaction (RRI) network. The RRI network is dominated by sRNAs, including predicted novel sRNAs, three of which we validated experimentally. We constructed a stringent subnetwork composed of RRIs that involve at least one hvKp virulence-associated gene and identified the capsule gene loci as a hub target where multiple sRNAs interact. We found that the sRNA OmrB suppressed both capsule production and hypermucoviscosity when overexpressed. Furthermore, OmrB base-pairs within kvrA coding region and partially suppresses translation of the capsule regulator KvrA. This agrees with current understanding of capsule as a major virulence and fitness factor. It emphasizes the intricate regulatory control of bacterial phenotypes by sRNAs, particularly of genes critical to bacterial physiology and virulence.
Interpretable prediction of mRNA abundance from promoter sequence using contextual regression models
Authors: Wang Song , Wang Wei 
Published: May 2024

Abstract
While machine learning models have been successfully applied to predicting gene expression from promoter sequences, it remains a great challenge to derive intuitive interpretation of the model and reveal DNA motif grammar such as motif cooperation and distance constraint between motif sites. Previous interpretation approaches are often time-consuming or have difficulty to learn the combinatory rules. In this work, we designed interpretable neural network models to predict the mRNA expression levels from DNA sequences. By applying the Contextual Regression framework we developed, we extracted weighted features to cluster samples into different groups, which have different gene expression levels. We performed motif analysis in each cluster and found motifs with active or repressive regulation on gene expression. By comparing the co-occurrence locations of discovered motifs, we also uncovered multiple grammars of motif combination including communities of cooperative motifs and distance constraints between motif pairs. These results revealed new insights of the regulatory architecture of promoter sequences.
Impact of Multidisciplinary Prehabilitation Interventions on Postoperative Hospital Length of Stay and Functional Capacity in Patients Undergoing Resection of Colorectal Cancer: A Systematic Review and Meta-analysis
Authors: Marmol-Perez Andres , Corres Pablo , Fernández-Escabias Manuel , Carrilho-Candeias Sofia , Ruiz Jonatan R. , Amaro-Gahete Francisco J. , Carneiro-Barrera Almudena 
Published: May 2024

BACKGROUND:
Although surgery is commonly regarded as the primary curative treatment for colorectal cancer, it could potentially be associated with postoperative morbidity and mortality.

OBJECTIVE:
To determine the pooled effect of exercise and multidisciplinary prehabilitation interventions on postoperative hospital length of stay and functional capacity in patients undergoing resection of colorectal cancer.

DATA SOURCES:
A systematic search was conducted in MEDLINE (via PubMed) and Web of Science databases from inception to November 2022.

STUDY SELECTION:
The original systematic search retrieved 2005 studies. After the removal of duplicates and screening by title and abstract, 77 eligible full-text documents were evaluated for final inclusion in the meta-analysis. A total of 12 randomized controlled trials, 5 nonrandomized controlled trials, and 3 uncontrolled before-and-after studies were selected.

MAIN OUTCOME MEASURES:
Postoperative hospital length of stay (in days) and functional capacity (assessed with the peak of oxygen consumption [VO2 peak] and 6-minute walking test) were the outcome measures.

RESULTS:
The meta-analysis was conducted on 20 studies (3805 participants). Randomized controlled trials and nonrandomized controlled trials showed significant reductions in postoperative hospital length of stay (d = –0.10, nearly 2 days) and significant incremental improvements in VO2 peak (d = 0.27) and 6-minute walking test (d = 0.31). Regarding the before-and-after studies, the pooled effect of multidisciplinary prehabilitation interventions was positively significant for VO2 peak (d = 0.29) and 6-minute walking test (d = 0.29). There was no risk of publication bias (Egger test: p > 0.05), with a score of 0.71 (0–1) on average.

LIMITATIONS:
There was a high between-studies heterogeneity, and several outcomes did not have the required number of studies for a desirable statistical power.

CONCLUSIONS:
These findings suggest that multidisciplinary prehabilitation interventions might be effective at decreasing postoperative hospital length of stay (nearly 2 days) and improving functional capacity.

REGISTRATION:
PROSPERO registration number CRD42022373982.