bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reassignment of the Structure of a Tryptophan‐Containing Cyclic Tripeptide Produced by the Biarylitide Crosslinking Cytochrome P450blt
Authors: Coe Laura J. , Zhao Yongwei , Padva Leo , Keto Angus , Schittenhelm Ralf , Tailhades Julien , Pierens Greg , Krenske Elizabeth H. , Crüsemann Max , De Voss James J. , Cryle Max J. 
Published: May 2024

AbstractThe structure of the sidechain crosslinked Tyr‐Leu‐Trp peptide produced by the biarylitide crosslinking cytochrome P450Blt from Micromonospora sp. MW‐13 has been reanalysed by a series of NMR, computational and isotope labelling experiments and shown to contain a C−N rather than a C−O bond. Additional in vivo experiments using such a modified peptide show there is a general tolerance of biarylitide crosslinking P450 enzymes for histidine to tryptophan mutations within their minimal peptide substrate sequences despite the lack of such residues noted in natural biarylitide gene clusters. This work further highlights the impressive ability of P450s from biarylitide biosynthesis pathways to act as biocatalysts for the formation of a range of sidechain crosslinked tripeptides.
Stable Axially Chiral Cyclohexylidenes from Catalytic Asymmetric Knoevenagel Condensation
Authors: Ying Meijia , Wang Kaixuan , Yan Wenjun , Pu Maoping , Lin Lili 
Published: May 2024

AbstractAxially chiral cycloalkylidenes are interesting but less developed axially chiral molecules. Here, a bispidine‐based chiral amine catalytic system was developed to promote efficiently the asymmetric Knoevenagel condensation of N‐protected oxindoles and benzofuranones with 4‐substituted cyclohexanones. A variety of alkylidenecycloalkanes with stable axial chirality were obtained in good yields and fairly good er (enantiomeric ratio). Based on the absolute configuration determination of product and DFT calculations, a possible mechanism of stereoselective induction was proposed.
Out of the Dark, into the Light: Metabolic Fluorescent Labeling of Nucleic Acids
Authors: Knaack J. Iven H. , Meier Chris 
Published: May 2024

AbstractThis review outlines recent advances in live‐cell imaging techniques for nucleic acids. We describe the evolution of these methods, particularly highlighting the development of metabolic labeling approaches compatible with living systems using fluorescence‐based labeling.
An Exploration of Cysteamine as a Subphase Additive for the Fabrication of Uniform Gold Nanorod Arrays using Langmuir‐Blodgett Deposition
Authors: Albarghouthi N. , Chotoye S. A. B. , Brosseau C. L. 
Published: May 2024

AbstractGold nanorods (AuNRs) have attracted significant attention over the past several decades for a variety of applications and there has been steady progress with regards to their synthesis and modification. Despite these advances, the assembly of AuNRs into well‐organized hierarchical assemblies remains a formidable challenge. Specifically, there is a need for tools that can fabricate assemblies of nanorods over large length scales at low cost with the potential for high‐throughput manufacturing. Langmuir‐Blodgettry is a monolayer deposition technique which has been primarily applied to amphiphilic molecules, but which has recently shown promise for the ordering of functionalized nanoparticles residing at the air‐water interface. In this work, Langmuir‐Blodgett deposition is explored for the formation of AuNR arrays for enhanced surface‐enhanced Raman spectroscopy (SERS) sensing. In particular, both surface modification of the AuNRs as well as subphase modification with cysteamine were evaluated for AuNR array fabrication.
An NMR Toolkit to Probe Amyloid Oligomer Inhibition in Neurodegenerative Diseases: From Ligand Screening to Dissecting Binding Topology and Mechanisms of Action
Authors: Palmioli Alessandro , Airoldi Cristina 
Published: May 2024

AbstractThe aggregation of amyloid peptides and proteins into toxic oligomers is a hallmark of neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, Machado‐Joseph's disease, and transmissible spongiform encephalopathies. Inhibition of amyloid oligomers formation and interactions with biological counterparts, as well as the triggering of non‐toxic amorphous aggregates, are strategies towards preventive interventions against these pathologies. NMR spectroscopy addresses the need for structural characterization of amyloid proteins and their aggregates, their binding to inhibitors, and rapid screening of compound libraries for ligand identification. Here we briefly discuss the solution experiments constituting the NMR spectroscopist‘s toolkit and provide examples of their application.
Investigation of Crystallographic, Morphological, Magnetic and Electrochemical Properties of La‐Doped Cu‐CoFe2O4 Spinel Ferrites
Authors: Khan Mohd Rouf , Mahajan Hamnesh , Srivastava A. K. 
Published: May 2024

AbstractThis research presents the successful synthesis of Cu0.79Co0.21LaxFe2‐xO4 (0.0 ≤ x ≤ 0.8) (spinel ferrite) nanoparticles via the sol‐gel auto combustion technique, with varying La3+ dopant concentrations. In this study, the estimated crystallite size (D) is found to be in the range of (27.92–40.90) nm. The microstructural parameter determination in XRD data is improved using Rietveld refinement. Fourier Transform Infrared Spectroscopy (FTIR) spectra exhibit two distinct metal stretching vibrational bands within (400–600) cm−1 range, a characteristic fingerprint region for all ferrites. Field Emission Scanning Electron Microscopy (FESEM) analysis reveals the agglomeration of particles due to magnetic interactions and non‐uniform distribution of average particle sizes ranging from (1.06–1.87) µm. Energy Dispersive X‐Ray Analysis (EDX) validates the chemical composition's accuracy. Owing to the dilution effect resulting from the introduction of non‐magnetic La3+ ions into the ferrite structure, there is a reduction in the saturation magnetization value, decreasing from 37.28 to 6.24 emu g−1 in the Vibrating Sample Magnetometery (VSM) study. The electrochemical analysis reveals the impressive electrochemical characteristics of the newly developed ferrites, highlighting a remarkable specific capacitance of 270.0 F g−1. This finding positions them as highly promising contenders for a wide range of energy storage supercapacitor applications.
Maximizing CSR impact: Leveraging artificial intelligence and process optimization for sustainability performance management
Authors: Khan Ali Nawaz , Mehmood Khalid , Ali Ahsan 
Published: May 2024

AbstractThis study, which is anchored to Resource‐Based View (RBV) theory, explores the relationship between artificial intelligence (AI), process optimization, organizational flexibility, and sustainability performance in organizational settings. Leveraging the RBV framework's focus on internal resources as sources of competitive advantage, this research seeks to clarify how AI adoption, process optimization, and organizational flexibility foster sustainable growth. SPSS PROCESS Macro was used to analyze the data from 288 organizations. The findings derived from the empirical data, this study verify the positive relationships between AI and process optimization as well as AI and sustainability performance, revealing the strategic nature of AI as a resource to improve operational effectiveness and environmental responsibility. Further, our results indicate the mediating role of process optimization and the moderating effect of organizational flexibility in determining the link between AI and sustainability outcomes. Moreover, results confirmed the indirect effects of AI on sustainability performance via process optimization under the boundary conditions of the organizational flexibility. These findings add to the developing literature of sustainable operational practices and provide practical suggestions that might be useful for the practitioners aiming at using AI driven by organizational capabilities to deliver sustainability performance.
In Situ Spectroelectrochemical Study of Acetate Formation by CO2 Reduction Using Bi Catalyst in Amine‐Based Capture Solution
Authors: Bohlen Barbara , Daems Nick , Su Zhangfei , Chen Aicheng , Lipkowski Jacek , Breugelmans Tom 
Published: May 2024

AbstractCarbon capture and utilization (CCU) are technologies sought to reduce the level of CO2 in the atmosphere. Industrial carbon capture is associated with energetic penalty, thus there is an opportunity to research alternatives. In this work, spectroelectrochemistry was used to analyze the electrochemical CO2 reduction (eCO2R) in CO2 saturated monoethanolamine (MEA)‐based capture solutions, in a novel CCU process. The in situ Fourier transform infrared (FTIR) spectroscopy experiments show that at the Bi catalyst, the active species involved in the eCO2R is the dissolved CO2 in solution, and not carbamate. In addition, the products of eCO2R were evaluated under flow, using commercial Bi2O3 NP as catalyst. Formate and acetate were detected, with normalized FE for acetate up to 14.5 %, a remarkable result, considering the catalyst used. Acetate is formed either in the presence of cetrimonium bromide (CTAB) as surfactant or at higher current density (>−100 mA cm−2) and the results enabled the proposition of a pathway for its production. This work sheds light on the complex reaction environment of a capture medium electrolyte and is thus relevant for an improved understanding of the conversion of CO2 into value‐added products and to evaluate the feasibility of a combined CCU approach.
Cathodic Conversion of Pressurized CO2 at Silver Cathodes: What are the Optimal Values of Pressure and Current Density?
Authors: Proietto Federica , Cammisa Giuseppe , Contino Mario , Inguanta Rosalinda , Galia Alessandro , Scialdone Onofrio 
Published: May 2024

AbstractThe cathodic reduction of pressurized CO2 (PrCO2CR) at suitable cathodes can allow to produce various chemicals, such as formic acid/formate (FA) and carbon monoxide or synthesis gas, with high faradic efficiencies (FEs) and productivities. Here, we have performed the conversion of CO2 in an undivided pressurized electrochemical reactor using silver cathode in order to determine the optimal values of CO2 pressure and current density. It was found that the plot FE vs. pressure resulted in a curve with a maximum. Similarly, an optimal value of current density can be selected for the PrCO2CR. The competition between the production of carbon monoxide and formic acid/formate is strongly affected by both the pressure and the current density. Eventually the effect of pressure and current density on the economic figures of the process was evaluated.
Cytopathology in the era of social media
Authors: Baskota Swikrity , Shaker Nada , Balassanian Ronald , Vohra Poonam 
Published: May 2024

AbstractIn today's medical landscape, social media (SoMe) platforms have expanded their reach beyond mere communication and entertainment, making a significant impact in the pathology field, including cytopathology. In recent years, SoMe platforms have become increasingly adopted by cytopathologists, facilitating continued education, professional networking, enhancing patient engagement, and entertainment. This adoption has influenced the professional growth of cytopathologists, and at its best, has led to the establishment of a robust professional online presence and ultimately contributed to leadership positions, fellowship opportunities, and academic promotions. Moreover, the integration of SoMe into the academic field has shown a profound impact on the visibility of academic journals and has provided a platform for lower‐impact factor journals to expand their reach, ultimately increasing article citation rates and positively contributing to journal impact factor growth. SoMe platforms created a modern avenue for conference networking that has revolutionized knowledge dissemination and enhanced real‐time engagement. The advantages of SoMe have extended to a global scale, positively enhancing professional expertise sharing, facilitating effective communication and teleconsultation worldwide, and reaching developing countries. Drawing insights from the recent medical literature and the practical insight from the experts' personal experience, this article provides a comprehensive review of how SoMe and cytopathology intersect to create new opportunities, facilitating informed discussions, global collaboration, and advancements in the field of cytopathology. This article also delves into the challenges surrounding SoMe platform navigation and addresses ethical and regulatory concerns, providing guidelines on what to post and what not to post on SoMe platforms.
Immunoglobulin G4 ‐related gastrointestinal disease associated with type 1 autoimmune pancreatitis: A case report
Authors: Sato Shingo , Kasai Toyotaka , Eto Hiroyuki , Okamoto Shikiko , Nagashima Ariki , Ushiyama Rui , Shimazaki Reiri , Nitta Hiroshi , Arai Motonori , Ito Hiroshi 
Published: May 2024

AbstractImmunoglobulin G4 (IgG4)‐related diseaseis a systemic inflammatory condition of unknown etiology characterized by increases in serum IgG4 and in the number of IgG4‐positive cells in affected tissues. One of the commonly involved locations is the pancreas; this condition is known as type 1 autoimmune pancreatitis (AIP). Type 1 AIP, which shows a biliary stricture in the intrapancreatic bile duct, can be misdiagnosed as a malignancy due to similar cholangiography findings and clinical presentation. In rare cases complicated by post‐bulbar duodenal ulcers, differentiating between type 1 AIP and malignancies is even more difficult. An 81‐year‐old male was referred to our hospital for the treatment of a pancreatic head mass and obstructive jaundice. Serological and radiological findings were consistent with both type 1 AIP and a malignancy. Gastroduodenoscopy revealed a post‐bulbar duodenal ulcer with endoscopic features that evoked malignant duodenal invasion. Although biopsies were negative for malignant cells, subsequent bleeding from the lesion suggested the progression of malignancy, which led to surgical resection. Pancreatoduodenectomy and pathological examination indicated that type 1 AIP was present. Simultaneously, the involvement of IgG4‐related disease in the ulcerative lesion was suggested. To our knowledge, this is the first reported case of type 1 AIP complicated by post‐bulbar duodenal ulcers, which was misdiagnosed as malignancy and considered an IgG4‐related gastrointestinal disease associated with type 1 AIP.
Trophic position determines the persistence of neotropical understory birds after forest disturbance
Authors: Paces Bernhard , Wanek Wolfgang , Voigt Christian C. , Schirmer Saskia , Leidinger Paulus , Schulze Christian H. 
Published: May 2024

AbstractHabitat loss and degradation are key drivers of the current biodiversity crisis. Most research focuses on the question of which traits allow species to persist in degraded habitats. We asked whether a species' trophic position or niche width influences the resilience of species in degraded habitats and to what extent habitat degradation affects trophic interactions between species. We used nitrogen isotope ratios (15N:14N, expressed as δ15N value) to quantify and compare trophic positions and niche widths of understory birds inhabiting old‐growth and young secondary forests in the Pacific lowlands of Costa Rica. We found that a species' trophic position rather than its trophic niche width determined its persistence in secondary forests. Species feeding at lower trophic levels in old‐growth forests were less likely to persist in secondary forests than those occupying a higher trophic position in old‐growth forests. This pattern is likely induced by the occurrence of relatively large‐bodied habitat specialists with a flexible and high‐trophic level diet in secondary forests. These habitat specialists likely caused generalist bird species to lower their trophic position relative to conspecifics in old‐growth forests. Regarding trophic niche widths, species in secondary forests tend to have larger niche widths than old‐growth forest species. However, as old‐growth forest specialists and generalists did not differ in their niche widths, no systematic effect of trophic niche width on species persistence after forest disturbance was found. This is the first study that shows a systematic effect of trophic position on the persistence of a wide range of bird species in a disturbed forest ecosystem. It therefore provides important insights into species' responses to habitat degradation and the conservation value of secondary forests. To improve habitat quality for old‐growth forest birds and facilitate avian seed dispersal, the creation of large contiguous forest patches should be prioritised when implementing reforestation measures.
Comparison of qPCR and metabarcoding for environmental DNA surveillance of a freshwater parasite
Authors: Johnson Mark , Tetzlaff Sasha , Katz Aron , Sperry Jinelle 
Published: May 2024

AbstractAnalysis of environmental DNA (eDNA) has been successfully used across freshwater ecological parasitology to inform management of ecologically and economically important species. However, most studies have used species‐specific quantitative polymerase chain reaction (qPCR) assays to detect target taxa. While generally effective, this approach limits the amount of community and management‐supporting data that can be obtained from eDNA samples. If eDNA metabarcoding could be conducted with the same accuracy of a single species approach, researchers could simultaneously detect a target species while obtaining vast community data from eDNA samples. We sampled 38 freshwater sites on Fort McCoy, Wisconsin and compared qPCR to metabarcoding for eDNA detection of the ectoparasitic gill louse Salmincola edwardsii, an obligate parasite of Salvelinus fishes (chars). We found no evidence to suggest S. edwardsii occupancy or detection probabilities differed between qPCR and metabarcoding. Further, we found that the number of S. edwardsii reads from metabarcoding were negatively predictive of CT values from qPCR (CT value indicates cycle a significant amount of target eDNA is detected, with lower CTs indicative of more DNA), demonstrating that our metabarcoding reads positively predicted qPCR DNA quantities. However, the number of reads was not predictive of overall qPCR score (number of positive qPCR replicates). In addition to S. edwardsii, metabarcoding led to the detection of a vast community of over 2600 invertebrate taxa. We underscore the necessity for conducting similar analyses across environments and target species, as the ecology of eDNA will vary on a per‐study basis. Our results suggest that eDNA metabarcoding provides a highly sensitive and accurate method for detecting parasitic gill lice while also illuminating the broader biological community and co‐occurrence of species in the environment.
Linking aerial hyperspectral data to canopy tree biodiversity: An examination of the spectral variation hypothesis
Authors: Crofts Anna L. , Wallis Christine I. B. , St‐Jean Sabine , Demers‐Thibeault Sabrina , Inamdar Deep , Arroyo‐Mora J. Pablo , Kalacska Margaret , Laliberté Etienne , Vellend Mark 
Published: May 2024

AbstractImaging spectroscopy is emerging as a leading remote sensing method for quantifying plant biodiversity. The spectral variation hypothesis predicts that variation in plant hyperspectral reflectance is related to variation in taxonomic and functional identity. While most studies report some correlation between spectral and field‐based (i.e., taxonomic and functional) expressions of biodiversity, the observed strength of association is highly variable, and the utility in applying spectral community properties to examine environmental drivers of communities remains unknown. We linked hyperspectral data acquired by airborne imaging spectrometers with precisely geolocated field plots to examine the spectral variation hypothesis along a temperate‐to‐boreal forest gradient in southern Québec, Canada. First, we examine the degree of association between spectral and field‐based dimensions of canopy tree composition and diversity. Second, we ask whether the relationships between field‐based community properties and the environment are reproduced when using spectral community properties. We found support for the spectral variation hypothesis with the strength of association generally greater for the functional than taxonomic dimension, but the strength of relationships was highly variable and dependent on the choice of method or metric used to quantify spectral and field‐based community properties. Using a multivariate approach (comparisons of separate ordinations), spectral composition was moderately well correlated with field‐based composition; however, the degree of association increased when univariately relating the main axes of compositional variation. Spectral diversity was most tightly associated with functional diversity metrics that quantify functional richness and divergence. For predicting canopy tree composition and diversity using environmental variables, the same qualitative conclusions emerge when hyperspectral or field‐based data are used. Spatial patterns of canopy tree community properties were strongly related to the turnover from temperate‐to‐boreal communities, with most variation explained by elevation. Spectral composition and diversity provide a straightforward way to quantify plant biodiversity across large spatial extents without the need for a priori field observations. While commonly framed as a potential tool for biodiversity monitoring, we show that spectral community properties can be applied more widely to assess the environmental drivers of biodiversity, thereby helping to advance our understanding of the drivers of biogeographical patterns of plant communities.
Primer set evaluation and sampling method assessment for the monitoring of fish communities in the North‐western part of the Mediterranean Sea through eDNA metabarcoding
Authors: Roblet Sylvain , Priouzeau Fabrice , Gambini Gilles , Dérijard Benoit , Sabourault Cécile 
Published: May 2024

AbstractEnvironmental DNA (eDNA) metabarcoding appears to be a promising tool to survey fish communities. However, the effectiveness of this method relies on primer set performance and on a robust sampling strategy. While some studies have evaluated the efficiency of several primers for fish detection, it has not yet been assessed in situ for the Mediterranean Sea. In addition, mainly surface waters were sampled and no filter porosity testing was performed. In this pilot study, our aim was to evaluate the ability of six primer sets, targeting 12S rRNA (AcMDB07; MiFish; Tele04) or 16S rRNA (Fish16S; Fish16SFD; Vert16S) loci, to detect fish species in the Mediterranean Sea using a metabarcoding approach. We also assessed the influence of sampling depth and filter pore size (0.45 μm versus 5 μm filters). To achieve this, we developed a novel sampling strategy allowing simultaneous surface and bottom on‐site filtration of large water volumes along the same transect. We found that 16S rRNA primer sets enabled more fish taxa to be detected across each taxonomic level. The best combination was Fish16S/Vert16S/AcMDB07, which recovered 95% of the 97 fish species detected in our study. There were highly significant differences in species composition between surface and bottom samples. Filters of 0.45 μm led to the detection of significantly more fish species. Therefore, to maximize fish detection in the studied area, we recommend to filter both surface and bottom waters through 0.45 μm filters and to use a combination of these three primer sets.