bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital twin-driven intelligent design and fabrication of the energy-efficient wiper prototype for electrical vehicle
Authors: Dong Guan , Wang Jie , Song Qiangwei , Wu Xiangjun , Li Kai 
Published: February 2025

Abstract
Electric vehicles (EVs) have gained popularity due to their efficiency, low emissions, and reduced noise. However, limited driving range, or range anxiety, remains a challenge, as all EV accessories rely on battery power. This study proposes a digital twin-driven methodology to design energy-efficient wipers, addressing this issue. Five driving modes are proposed, the authors analyzed wiper motor energy consumption through both experimental test and digital modeling, and compared with the traditional wiper drive mode, Results show potential efficiency improvements of up to 33%, demonstrating significant energy-saving opportunities. We also examine wiper vibration properties and develop contact models to create quieter prototypes. This research guides the design of energy-efficient EV accessories, potentially easing range anxiety and improving overall performance. The approach could be applied to other EV components to further enhance efficiency and driving range.&#xD;
Poetry's Nature
Authors: Stewart Susan 
Published: February 2025

Abstract
Though it is common to speak of “nature poetry,” Poetry’s Nature contends that the essential nature of poetry is bound up with the natural world: by looking to nature, we can better understand poetry, and—perhaps—vice versa. The study draws on contemporary physics and philosophy to argue that all beings, and all matter, are enmeshed in relations to one another, and that such processual relations can help us to think about poetry as an ever-arriving, ever-unfinished art. The poem may then be a microcosm of the world: parts are grounded by their relation to one another, always in the process of creating a whole. Poetry’s Nature’s four chapters explore four paradigms that illuminate poetry’s relation to other natural phenomena: the ways poems draw on birdsong to veer between language and sound, and hence between semantic density and meaninglessness; the experience of seasonality as a paradigm for the lyric’s recursive use of time; the flows and forms of water as an inspiration for the enactment and depiction of motion and rest in poems; and, finally, the vast domain of the imperceptible as a resource for the imagination. Poems are events that are felt in time rather than being merely cognized; rewarding of our attention, like the natural world; experienced, like the weather, in our bodies. By reframing poetry in its relation to nature, Poetry’s Nature hopes to reframe our relation to the world in which we live, a task that is of ever-greater urgency.
What Was the Ark and What Did It Do?
Authors: McGeough Kevin M 
Published: February 2025

Abstract
This chapter describes what the Bible tells us about the Ark. It discusses the narrative traditions surrounding the Ark, emphasizing the different political and social roles that the Ark played in different episodes recounted in the Old Testament. The chapter surveys how God commanded Moses to build it and how the Ark was carried by the Israelites as they wandered through the wilderness. According to the Bible, it played a role in the conquest of the Land. The Ark was briefly lost to the Philistines, but it was eventually installed in Jerusalem as that city became the capital of David’s kingdom. The various elements of the Ark’s physical description are explained, as are areas of scholarly disagreement about what it looked like. The chapter discusses the different Hebrew names used for the Ark and its treatment in different biblical sources.
Puddling in butterflies: current knowledge and new directions
Authors: Lamie Emily , Morton Elise R , Parzer Harald F 
Published: February 2025

Abstract
Puddling, a behavior in which butterflies and other insects aggregate on mineral-rich substrates such as mud, animal excrement, carrion, and even human perspiration, is a well-documented phenomenon in both temperate and tropical regions. This behavior is thought to compensate for the limited access to micronutrients in the adult diet that are important for fitness, primarily sodium and nitrogen, essential for nervous system function, growth, and reproductive processes. In this review, we consolidate existing knowledge on butterfly puddling and highlight key gaps in our understanding. The review is organized into 4 main themes: the benefits of puddling, social dynamics in puddling, sensory cues for site selection, and behavioral variation across species and regions. We explore how nutrient preferences are influenced by diet, environmental factors, and evolutionary history, with a particular focus on the role of sodium and nitrogen. We also discuss the potential reproductive benefits of puddling, including its impact on mating success and fecundity. In addition, we consider the sensory and social mechanisms butterflies use to locate suitable puddling sites. Lastly, we give a short overview on puddling in moths. This review identifies several areas for future research, particularly in understanding the adaptive significance of puddling across different taxa and environments.
Time in Target Range, All-cause Mortality and Cardiovascular Diseases
Authors: Wu Zhijun , Huo Zhenyu , Huang Zhe , Zhao Xiujuan , Wang Guodong , Ruan Chunyu , Chen Shuohua , Wu Shouling 
Published: February 2025

Abstract

BACKGROUND
Time in target range (TTR) of systolic blood pressure (SBP) is a concept characterizing the extent of blood pressure (BP) control. We aimed to compare the risk of all-cause mortality and cardiovascular diseases (CVDs) in participants with &gt;50% time of their SBP within different target ranges.

METHODS
A total of 40,914 participants who had complete BP data and were free of CVDs or cancer were included. Four SBP records over 6 years were used to calculate TTR and mean levels of covariates. The death and CVD events were recorded via reviewing medical records and death certificates.

RESULTS
During a median follow-up period of 8.78 (interquartile range: 8.50-9.05) and 7.76 (interquartile range: 7.46-8.04) years, we identified 2,398 death cases and 1,995 incident CVDs, respectively. Participants who had &gt;50%TTR &gt;150mmHg had a higher risk of all-cause mortality (hazard ratio (HR):1.67, 95% confidence interval (CI): 1.16, 2.41) than those with &gt;50% TTR &lt;120mmHg. The corresponding HR was 2.06 (95%CI: 1.37,3.10) for incident CVDs. The increased risk of all-cause mortality and CVD events persisted when we adjusted the covariates measured at the 2012 visit or excluded coalminers, the hypotensive participants, the incident cases occurring the first 2 years or who used BP lowering drugs.

CONCLUSIONS
Our study suggested that &gt;50% TTR in a high target range of SBP significantly predicted the occurrence of all-cause mortality and CVDs.
Human-induced pheromone pollution leads to changes in alternative mating tactics of moths
Authors: Waner Rips Shevy , Motro Michal , Motro Uzi , Kolodny Oren , Harari Ally 
Published: February 2025

Abstract
Environmental changes driven by anthropogenic activities often disrupt animal communication and mating behavior. Consequently, these changes may force animals to adopt alternative mating tactics and strategies to find a mate. The mating disruption technique is an environmentally friendly tactic often used to control the pink-bollworm moth population in cotton fields. Though mating disruption is eco-friendly, it represents a Human-Induced Rapid Environmental Change for the targeted moths. Mating disruption involves spreading a synthetic version of the species-specific sex pheromone in the field, creating a pheromone-polluted environment, making it difficult for male moths to locate females and thereby reducing mating rates. We hypothesized that the intense sexual selection and environmental changes affecting communication would lead moths to increase their use of alternative mating strategies. An observed alternative mating behavior in male pink bollworm moths is disturbing mating pairs to displace the male and mate with the female. We compared this behavior between two populations and found that males long exposed to mating disruption disturbed mating pairs more frequently than those never exposed to it. In addition, males with a prolonged history of exposure to mating disruption showed reduced choosiness of females and increased their mating rate with small females of lower reproductive potential. The success rate of the observed couple disturbance was low. Nonetheless, this strategy, alongside other strategies, may contribute to the males’ reproductive success when facing the additional challenge of locating females due to the pheromone-polluted environment.
ELLIPSIS: Robust quantification of splicing in scRNA-seq
Authors: Van Hecke Marie , Beerenwinkel Niko , Lootens Thibault , Fostier Jan , Raedt Robrecht , Marchal Kathleen 
Published: February 2025

Abstract

Motivation
Alternative splicing is a tightly regulated biological process, that due to its cell type specific behaviour, calls for analysis at the single cell level. However, quantifying differential splicing in scRNA-seq is challenging due to low and uneven coverage. Hereto, we developed ELLIPSIS, a tool for robust quantification of splicing in scRNA-seq that leverages locally observed read coverage with conservation of flow and intra-cell type similarity properties. Additionally, it is also able to quantify splicing in novel splicing events, which is extremely important in cancer cells where lots of novel splicing events occur.

Results
Application of ELLIPSIS to simulated data, proves that our method is able to robustly estimate Percent Spliced In values in simulated data, and allows to reliably detect differential splicing between cell types. Using ELLIPSIS on glioblastoma scRNA-seq data, we identified genes that are differentially spliced between cancer cells in the tumor core and infiltrating cancer cells found in peripheral tissue. These genes showed to play a role in a.o. cell migration and motility, cell projection organization and neuron projection guidance.

Availability and implementation
ELLIPSIS quantification tool: https://github.com/MarchalLab/ELLIPSIS.git

Supplementary Information
Supplementary data are available at Bioinformatics online.
Detecting residual disease after neoadjuvant chemoradiotherapy for oesophageal squamous cell carcinoma: prospective multicentre preSINO trial
Authors: Yang Yang , Liu Zhichao , Wong Ian , Gao Xing , Zhang Hong , Liu Jun , Eyck Ben M , Shao Jinchen , Han Yuchen , van der Wilk Berend J , Chao Yinkai , Law Simon , Wijnhoven Bas P L , van Lanschot J Jan B , Li Zhigang 
Published: February 2025

Abstract

Background
Neoadjuvant chemoradiotherapy (nCRT) in patients with oesophageal squamous cell carcinoma (OSCC) may lead to clinical complete response (cCR). It is important to know the accuracy of clinical response evaluations (CREs) before advocating active surveillance instead of oesophagectomy.

Methods
This was a prospective, multicentre study of patients with locally advanced OSCC. They received the first CRE (bite-on-bite biopsies) 4–6 weeks after nCRT. Patients with residual tumour underwent surgery. Patients with a cCR at CRE-1 underwent a second CRE 10–12 weeks after nCRT using PET-CT, bite-on-bite biopsies and endoscopic ultrasound fine-needle aspiration (EUS-FNA). All patients without distant metastases underwent surgery. Primary endpoint was the accuracy of CREs for detecting Tumour Regression Grade (TRG)3–4 or TRG1–2 with ypN+ residual tumour with a prespecified false-negative rate (FNR) of 19.5%. Circulating-tumour DNA (ctDNA) at CREs was performed for exploratory analysis.

Results
In total 309 patients were included. Eighteen of 133 patients with TRG3–4 or TRG1–2 with ypN+ residual tumours were not detected by bite-on-bite biopsies and EUS-FNA (FNR: 13.5%). Sensitivity, specificity, negative predictive value and positive predictive value of detecting any residual tumour were 81.7%, 93.2%, 68.7% and 96.5% respectively. PET-CT detected interval distant metastases in 13 (4.9%) of 268 patients presurgically. After a minimum 12-month follow-up, systemic recurrence rates were 28.0% in patients with positive ctDNA at CREs and 5.3% in those with negative ctDNA.

Conclusions
Bite-on-bite biopsies and EUS-FNA were accurate in detecting residual disease after nCRT in OSCC. Positive ctDNA at CREs may indicate an increased risk of systemic metastases.
Association of plasma soluble urokinase plasminogen activator receptor concentrations and migraine with aura: a REFORM study
Authors: Tesfay Betel , Ashina Håkan , Christensen Rune Häckert , Al-Khazali Haidar M , Karlsson William Kristian , Amin Faisal Mohammad , Jawad Baker Nawfal , Andersen Ove , Ashina Messoud 
Published: February 2025

Abstract
Soluble urokinase plasminogen activator receptor (suPAR) has garnered attention as a potential blood-based biomarker for low-grade chronic inflammation. However, its specific association with migraine, including its subtypes, remains to be elucidated. We sought to examine the association of plasma suPAR levels with migraine and its subtypes. In this single-centre, cross-sectional study, plasma was collected at a single time point in adults with migraine and sex-matched healthy controls from October 2020 to June 2022. The quantification of plasma suPAR levels was performed in a blinded fashion using a validated enzyme-linked immunosorbent assay. Plasma suPAR levels were compared between participants with migraine (including subgroups) and healthy controls. Plasma samples were analysed from 634 eligible participants with migraine [mean (SD) age, 44.0 (12.2) years; 568 (89.6%) females] and 154 healthy controls [mean (SD), 41.3 (11.8%) years; 132 (86%) females]. Plasma suPAR levels were 6.7% higher (95% CI: 0.1–13.6%; P = 0.045, adjusted for age, sex, body mass index and smoking) in participants with migraine with aura, when compared with healthy controls. Further analysis revealed no difference in plasma suPAR levels between the overall migraine group and healthy controls (3.7%; 95% CI: −0.7–8.2%; P = 0.097), as well as between participants with migraine without aura and healthy controls (2.5%; 95% CI: −2.9–8.3%; P = 0.81). Similarly, plasma suPAR levels did not differ across participants with episodic migraine, chronic migraine and healthy controls. Finally, we found no difference when comparing participants with migraine at time of blood sampling with participants with non-migraine headache (1.0%; 95% CI: −5.7–8.2; P &gt; 0.99), participants without headache (1.2%; 95% CI: −4.2–7.0%; P &gt; 0.99) or healthy controls (4.5%; 95% CI: −1.9–11.3%; P = 0.39). Elevated plasma suPAR levels in migraine with aura indicate the presence of low-grade chronic inflammation. Future research should explore the role of suPAR in the neurobiologic underpinnings of migraine with aura.
Cortico-hippocampal networks underpin verbal memory encoding in temporal lobe epilepsy
Authors: Fiore Giorgio , Giampiccolo Davide , Xiao Fenglai , Koepp Matthias J , Iglesias Juan E , Vos Sjoerd B , de Tisi Jane , McEvoy Andrew W , Bertani Giulio A , Locatelli Marco , Finn Roisin , Caciagli Lorenzo , Sidhu Meneka , Galovic Marian , Baxendale Sallie , Duncan John S , Miserocchi Anna 
Published: February 2025

Abstract
Knowledge of the structural underpinnings of human verbal memory is scarce. Understanding the human verbal memory network at a finer anatomical scale will have important clinical implications for the management of patients with verbal memory impairment.
In this cross-sectional study, we aimed to assess the contributions of cerebral cortex and hippocampal subfields to verbal memory encoding in temporal lobe epilepsy. We included consecutive patients (n = 84) with radiologically and pathologically defined hippocampal sclerosis (44 left-sided) and unilateral temporal lobe epilepsy, and healthy volunteers (n = 43) who were comparable regarding age and sex. The morphometric and volumetric measures of cerebral cortex and hippocampal subfields were extracted from high-resolution MRI scans. People included in this study underwent standardized neuropsychological evaluation, including measures of verbal memory assessed through the Adult Memory and Information Processing Battery. Verbal memory performances were z-scores corrected by using means and standard deviations published for sample standardization. Associations between verbal learning z-scores and the gray matter volume of the cerebral cortex and hippocampal subfields were investigated.
Reduction of gray matter volumes in the left and right medial and dorsolateral pre-frontal cortex (pcorr &lt; 0.0001), superior and middle temporal gyri (pcorr &lt; 0.0001), anterior and posterior cingulate cortex (pcorr &lt; 0.0001), and of the left ventrolateral PFC (pcorr &lt; 0.0001) and parietal-temporal-occipital junction (pcorr &lt; 0.0001) were associated with worse verbal learning. These findings were consistent across both the entire cohort and in a subgroup analysis focused exclusively on hippocampal sclerosis patients. Within hippocampi, smaller volumes of the left dentate gyrus (p = 0.003), cornu ammonis 4 (p = 0.005), and cornu ammonis 3 (p = 0.03) were associated with worse verbal learning z-scores.
This study demonstrates that verbal learning in patients with temporal lobe epilepsy is strongly related to the volume of distinct regions of the prefrontal, temporal, and cingulate cortices and left dentate gyrus, cornu ammonis 4, and cornu ammonis 3 hippocampal subfields. It provides the basis to suggest a corticohippocampal network for verbal learning in these patients, improving our understanding of human verbal memory. These biomarkers may inform attractive targets for forthcoming modulating therapies. Future work may also analyze the impact of sparing part of the left dentate gyrus, cornu ammonis 4, and cornu ammonis 3 as a protective measure against verbal memory impairment after surgery for temporal lobe epilepsy.
Vitamin B6 catabolism and psoriasis risk: A cross-sectional study
Authors: Bai Ruimin , Cheng Xiaoqing , Yang Yajie , Zhang Jian , Tian Qiong 
Published: February 2025

Abstract

Background
Psoriasis is a common autoimmune inflammatory disease. Vitamin B6 is crucial for the body’s inflammatory response, yet the relationship between pyridoxal 5'-phosphate (PLP), 4-pyridoxic acid (4-PA), and vitamin B6 turnover (4-PA/PLP) in psoriasis remains unexplored.

Objective
To investigate the relationship of PLP, 4-PA and vitamin B6 catabolism with the risk of psoriasis.

Methods
This cross-sectional study analyzed 7,540 participants from the National Health and Nutrition Examination Survey. Vitamin B6 catabolism was assessed via the serum 4-PA to PLP ratio (4-PA/PLP). The primary outcome was psoriasis, evaluated using weighted univariate and multivariate logistic regression to determine odds ratios (OR) and 95% confidence intervals (CI). Subgroup analyses were performed by age, gender, body mass index (BMI), hypertension, dyslipidemia, and cardiovascular disease (CVD).

Results
Out of the participants, 208 had psoriasis. After adjusting for confounders, 4-PA levels in cutoff above group were positively associated with psoriasis (OR=1.51, 95% CI: 1.03-2.20). Additionally, 4-PA/PLP correlated with increased psoriasis risk (OR=1.82, 95% CI: 1.02-3.26). However, PLP levels did not show a significant association. The positive link between 4-PA/PLP and psoriasis was consistent in individuals with BMI ≥25 kg/m², hypertension, and those without dyslipidemia.

Conclusion
The results revealed significant association of 4-PA and 4-PA/PLP level with the presence of psoriasis. However, further extensive prospective studies are necessary to establish causality.
Troponin I is an independent predictor of cardiovascular events and mortality in haemodialysis patients
Authors: Tydén Maria , Westerlund Magnus E , Duarte Kevin , Eriksson Niclas , Girerd Nicolas , Krämer Bernhard K , März Winfried , Rossignol Patrick , Scharnagl Hubert , Soveri Inga , Svensson Maria K , Zannad Faiez , Fellström Bengt 
Published: February 2025

Abstract

Background
Patients with end-stage kidney disease (ESKD) undergoing haemodialysis (HD) have a high risk of cardiovascular (CV) events. This study evaluated Troponin I (hs-cTnI) as a predictor of major adverse cardiac events (MACE), CV- and all-cause death.

Methods
The AURORA trial, a multicentre, randomized, double-blind trial involved 2776 HD patients comparing Rosuvastatin to placebo. No significant effect was found on the composite primary endpoint of CV death, nonfatal myocardial infarction or nonfatal stroke. In this post-hoc analysis, we analysed the association between baseline hs-cTnI and outcomes using Cox-regression analyses. We adjusted for multiple background factors and available biomarkers. Hs-cTnI was log2 transformed and modelled using a four-knot restricted cubic spline. Variables were ordered by their importance in the models using χ2 value minus degrees of freedom.

Results
Baseline median hs-cTnI was 17.3 pg/mL. During follow-up, 734 MACE, 598 CV deaths, and 1094 total deaths occurred. Patients in the upper quartile of hs-cTnI (&gt;32.6 pg/mL) had significantly higher risk of MACE (HR 1.92; 95% Confidence interval [CI] 1.57-2.35), CV death (HR 2.12; 95% CI 1.69-2.66), all-cause death (HR 1.84; 95% CI 1.55-2.17) and non-CV death (HR 1.59; 95% CI 1.23-2.05) after full adjustment compared to those in the lowest quartile (&lt;10.1pg/mL). Hs-cTnI was identified as the strongest predictor for MACE, CV death, and all-cause death, but not for non-CV death.

Conclusion
Baseline hs-cTnI is a strong and independent predictor for MACE and death in patients end-stage kidney disease (ESKD) undergoing haemodialysis (HD).
Hormonal regulation of human adipose tissue lipolysis: impact of adipose GIP system in overweight and obesity
Authors: Brachs Sebastian , Soll Dominik , Beer Finja , Huckauf Nadine , Konkar Anish , Spranger Joachim , Rütten Hartmut , Mai Knut 
Published: February 2025

Abstract

Objective
Given the promising effects of GLP-1/GIP/glucagon receptor triagonists on weight loss in animals and humans, improved understanding of underlying mechanism is required. We investigated a direct lipolytic effect of a specific GLP-1/GIP/glucagon receptor triagonist on human adipose tissue to disentangle central and peripheral effects as potential drivers of weight loss.

Design and methods
Isolated primary adipocytes from subcutaneous adipose tissue biopsies of 22 non-diabetic subjects [63.0 (57.0-69.5) years] were incubated with increasing concentrations of isoprenaline, GLP-1, GIP, glucagon, or a GLP-1/GIP/glucagon receptor triagonist. Glycerol concentration was measured following stimulation to assess lipolysis. mRNA expression of adipose tissue receptors was analyzed in parallel.

Results
Glycerol concentration only increased by isoprenaline, GIP (+13%), and GLP-1/GIP/glucagon receptor triagonist (+28%) but not by GLP-1 or glucagon. This effect was not related to age or body mass index (BMI). Higher adipose tissue GIP receptor mRNA expression was related to elevated glycerol release after GIP and GLP-1/GIP/glucagon receptor triagonist stimulation.

Conclusions
Direct lipolytic effects of GIP seem to exist in human subcutaneous adipose tissue. This might be targetable by multiple receptor agonists, especially with a high GIP receptor affinity. Such a mechanism can potentiate the beneficial effect on weight loss and will therefore represent a promising target of future research.

Clinical trial registration number
The trial was registered at German Clinical Trials Register (drks.de) as DRKS00010049.