bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic resistance profile in sediments and bacterial isolates from a human-impacted mangrove from Bahía Magdalena, Mexico
Authors: García-Martínez Stephany , Zapién-Chavarría Karen A , Rivera-Chavira Blanca E , Adame-Gallegos Jaime R , Palacios Oskar A , Nevárez-Moorillón Guadalupe V 
Published: April 2024

Abstract
Antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) in the environment are considered emerging contaminants. One of the ecosystems that can harbor resistant bacteria and genes is mangroves. This report aims to assess ARB and ARG's presence at two mangrove locations in Bahia Magdalena, Mexico. Bacterial isolates were selected based on their phenotypic profiles of antibiotic resistance. DNA from mangrove sediments and bacterial isolates was tested for int1 and antibiotic resistance genes by final point PCR. From 90 ARB isolates, the phenotypic profile showed resistance to ciprofloxacin (90%), cefotaxime (85.7%), and ampicillin (78.6%), while the beta-lactamase gene (blaCTX-M) was present in 72.9% of the isolates. Antibiotic resistance genes identified in sediments were found even at 30–50 cm depth, but the integron Class gen (int1) was found mainly in shallow samples. Principal component analysis showed a close relationship between the integron gene, vancomycin, and sulfonamides resistance genes. No correlation was found between the phenotypic and genotypic profiles of the bacterial isolates. Also, no differences were observed between the two mangrove ecosystems studied. The impact of anthropogenic activities was equally significant on both sites. The role of mangrove ecosystems in the dispersion of antibiotic resistance needs to be further explored.
A phylogenomic approach to a taxonomic revision: a combination, new synonymies, and a description of two new species within the camel spider genus Chanbria Muma, 1951 (Solifugae: Eremobatidae)
Authors: Garcia Erika L , Hansen Quincy G , Castillo Jaír R 
Published: April 2024

Abstract
This study summarizes the taxonomic treatment of the camel spider genus Chanbria Muma, 1951. Taking an integrative taxonomic approach incorporating phylogenomic, morphological, and geographical information, the genus is herein revised. Of the four species currently placed in the genus, two are retained: Chanbria regalis Muma, 1951 and Chanbria serpentinus Muma, 1951. Eremochelis plicatus (Muma, 1962) is transferred to this genus because it is consistently recovered in a clade with Chanbria based on several phylogenetic analyses using hundreds of loci recovered from ultraconserved element data. In this study, we re-analyse previously acquired genomic data to assess former species hypotheses and identify new morphological synapomorphies that support the monophyly of Chanbria. The genetic data support the synonymization of Chanbria rectus Muma, 1962 syn. nov. with C. regalis. Furthermore, we synonymize Chanbria tehachapianus Muma, 1962 syn. nov. with C. regalis because C. tehachapianus was erected based on limited morphological information and lack of geographical separation between other populations of C. regalis. Two new species, Chanbria brookharti sp. nov. and Chanbria mapemes sp. nov., are described. This brings the total number of species of Chanbria described to five recognized species: C. regalis, C. serpentinus, C. plicatus com. nov., C. brookharti sp. nov., and C. mapemes sp. nov.
Single‐cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization
Authors: Jia Shuai , Zhao Fei 
Published: April 2024

AbstractThe upper Müllerian duct (MD) is patterned and specified into two morphologically and functionally distinct organs, the oviduct and uterus. It is known that this regionalization process is instructed by inductive signals from the adjacent mesenchyme. However, the interaction landscape between epithelium and mesenchyme during upper MD development remains largely unknown. Here, we performed single‐cell transcriptomic profiling of mouse neonatal oviducts and uteri at the initiation of MD epithelial differentiation (postnatal day 3). We identified major cell types including epithelium, mesenchyme, pericytes, mesothelium, endothelium, and immune cells in both organs with established markers. Moreover, we uncovered region‐specific epithelial and mesenchymal subpopulations and then deduced region‐specific ligand‐receptor pairs mediating mesenchymal‐epithelial interactions along the craniocaudal axis. Unexpectedly, we discovered a mesenchymal subpopulation marked by neurofilaments with specific localizations at the mesometrial pole of both the neonatal oviduct and uterus. Lastly, we analyzed and revealed organ‐specific signature genes of pericytes and mesothelial cells. Taken together, our study enriches our knowledge of upper MD development, and provides a manageable list of potential genes, pathways, and region‐specific cell subtypes for future functional studies.
Characteristics of Hospitals by Graduate Medical Education Expense Category: Implications for Rural Residency Program Expansion
Authors: Adhikari Mukesh , Hawes Emily M. , Sanner Louis , Holmes George M. 
Published: April 2024

Abstract

Purpose
To describe how the characteristics of the hospitals and communities they serve vary across the 4 hospital graduate medical education (GME) expense categories (according to Section 131 of the Consolidated Appropriations Act of 2021) and identify the rurally located never claimer hospitals that are most similar to teaching hospitals, signaling that they might be good candidates for new rural GME programs.

Method
Hospital categories and characteristics were gathered from the March 2022 Medicare Cost Reports; 2022 County Health Rankings & Roadmaps data were used for community characteristics. Each acute hospital was classified into 1 of the following 4 mutually exclusive hospital categories: category A, category B, established teaching hospital (ETH), and never claimer. Multinomial logistic regressions were conducted to estimate the adjusted associations of hospital characteristics with hospital categories and to identify the never claimer hospitals in rural locations that have characteristics similar to teaching hospitals (category A, category B, and ETHs).

Results
Out of 3,590 hospitals, 2,075 (57.8%) were never claimer hospitals. After adjusting for multiple characteristics, rural hospitals had a similar probability of being in each hospital category to that of urban hospitals. Never claimer hospitals served an older population and were located in communities with more uninsured adults and children and less availability of primary care physicians, dentists, and mental health professionals.

Conclusions
This study demonstrated that most hospitals in every category, but especially teaching hospitals (i.e., category A hospitals, category B hospitals, and ETHs), were concentrated in urban areas. Larger hospitals (measured by net patient revenue) were more likely to report GME expenses (i.e., be a category A hospital, a category B hospital, or an ETH). The study suggests that there are roughly 145 rural never claimer hospitals that might be strong candidates for initiating new residency programs.
Graduating Medical Students’ Perceptions of the COVID-19 Pandemic’s Impact on Their Medical School Experiences and Preparation for Careers as Physicians: A National Study
Authors: Jayas Amy , Shaull Lynn , Grbic Douglas , Andriole Dorothy A. , McOwen Katherine S. 
Published: April 2024

Abstract

Purpose
The COVID-19 pandemic resulted in unprecedented changes to the medical education learning environment. The graduating class of 2021 was particularly affected. To better understand how students were affected, the authors explored positive and negative experiences described by graduating U.S. medical students.

Method
Using the conceptual framework of affordances, the authors examined narrative responses to 2 new open-ended questions on the Association of American Medical Colleges 2021 Graduation Questionnaire in which graduating students described the pandemic’s positive and negative effects on their medical school experiences and career preparation. Conventional content analysis was used to identify affordances and themes in responses.

Results
Of 16,611 Graduation Questionnaire respondents, 8,926 (54%) provided narrative responses. Of these 8,926 respondents, responses from 2,408 students (27%) were analyzed. Students described positive and negative perceptions of affordances, centering around virtual learning, social connection, and transition to residency. Fewer in-person and increased virtual clinical rotations offered students flexible scheduling, allowing them greater opportunity to explore academic and professional interests. Additionally, students’ sense of isolation from their medical school was alleviated when schools exercised intentional open communication and student involvement in decision making. Although many described a diminished sense of preparedness for residency due to limited access to in-person clinical experiences and to assessing residencies largely through virtual away rotations and interviews, the sense of community, adaptability, and resiliency gained through varied activities reinforced students’ professional identity and commitment to medicine during a time of much uncertainty.

Conclusions
This national exploration of students’ pandemic experiences highlights what students value as fundamental elements of medical education and student support and describes how innovation can improve long-standing practices. These findings, guided by students’ insights, can help inform the work of educational leaders as they consider which innovations should remain to continue enhancing student learning, engagement, and well-being.
Education Scholarship Assessment Reconsidered: Expansion of Glassick’s Criteria to Incorporate Health Equity
Authors: Bockrath Robyn , Osman Cynthia , Trainor Jennifer , Wang Helen C. , Phatak Uma Padhye , Richards Daniel G. , Keeley Meg , Chung Esther K. 
Published: April 2024

Abstract
Recent events have ignited widespread attention to structural racism and implicit bias throughout the U.S. health care system and medical institutions, resulting in a call for antiracism approaches to advance health equity. Medical education leaders are well positioned to advance health equity, not only through their training of fellows, residents, and medical students, but also in their approach to scholarship. Education scholarship drives innovation and critical evaluation of current practices; it impacts and intersects with multiple factors that have the potential to reduce health inequities. Thus, it is critical to prioritize the assessment of education scholarship through a health equity lens. Medical education scholarly dissemination has markedly expanded over the past 2 to 3 decades, yet medical educators have continued to embrace Boyer’s and Glassick and colleagues’ definitions of scholarship. The authors propose an approach to medical education scholarship assessment that expands each of Glassick’s 6 existing criteria to address health inequities and adds health equity as a seventh criterion. With this, medical educators, researchers, reviewers, and others can consider how education scholarship affects diverse populations and settings, direct educational products and scholarship to address health inequities, and raise the importance of advancing health equity in medical education scholarship. By expanding and standardizing the assessment of scholarship to incorporate health equity, the medical education community can foster a cultural shift that brings health equity to the forefront of education scholarship.
Comments and illustrations of the European Federation of Societies for Ultrasound in Medicine contrast-enhanced ultrasound guidelines. Rare pancreatic tumors, imaging features on transabdominal ultrasound and EUS with contrast enhancement: Rare epithelial pancreatic tumors: solid pseudopapillary neoplasm, acinar cell carcinoma, mixed neuroendocrine-non-neuroendocrine neoplasms, some rare subtypes of pancreatic adenocarcinoma and pancreatoblastoma
Authors: Möller Kathleen , Löwe Axel , Jenssen Christian , Bhutani Manoop S. , On Wei , Everett Simon M. , Braden Barbara , Hocke Michael , Healey Andrew , Dong Yi , Gerber Michael , Faiss Siegbert , Rimbas Mihai , Ge Nan , Sun Siyu , Taut Heike , Srivastava David , Burmester Eike , Dietrich Christoph F. 
Published: April 2024

ABSTRACT
Rare malignant pancreatic lesions are systematically reported in this review. The focus is on the imaging appearance of the rare epithelial pancreatic tumors such as the solid pseudopapillary neoplasm, acinar cell carcinoma, rare subtypes of adenocarcinoma, and pancreatoblastoma as seen on ultrasound, EUS, and contrast-enhanced ultrasound or EUS. The present overview summarizes the data and shows that not every pancreatic tumor is likely to be the most common entities of ductal adenocarcinoma or neuroendocrine tumor.
Benchmarking clinical risk prediction algorithms with ensemble machine learning for the noninvasive diagnosis of liver fibrosis in NAFLD
Authors: Charu Vivek , Liang Jane W. , Mannalithara Ajitha , Kwong Allison , Tian Lu , Kim W. Ray 
Published: April 2024

Background and Aims:
Ensemble machine-learning methods, like the superlearner, combine multiple models into a single one to enhance predictive accuracy. Here we explore the potential of the superlearner as a benchmarking tool for clinical risk prediction, illustrating the approach to identifying significant liver fibrosis among patients with NAFLD.

Approach and Results:
We used 23 demographic/clinical variables to train superlearner(s) on data from the NASH-clinical research network observational study (n = 648) and validated models with data from the FLINT trial (n = 270) and National Health and Nutrition Examination Survey (NHANES) participants with NAFLD (n = 1244). Comparing the superlearner’s performance to existing models (Fibrosis-4 [FIB-4], NAFLD fibrosis score, Forns, AST to Platelet Ratio Index [APRI], BARD, and Steatosis-Associated Fibrosis Estimator [SAFE]), it exhibited strong discriminative ability in the FLINT and NHANES validation sets, with AUCs of 0.79 (95% CI: 0.73–0.84) and 0.74 (95% CI: 0.68–0.79) respectively.

Conclusions:
Notably, the SAFE score performed similarly to the superlearner, both of which outperformed FIB-4, APRI, Forns, and BARD scores in the validation data sets. Surprisingly, the superlearner derived from 12 base models matched the performance of one with 90 base models. Overall, the superlearner, being the “best-in-class” machine-learning predictor, excelled in detecting fibrotic NASH, and this approach can be used to benchmark the performance of conventional clinical risk prediction models.
Kupffer cell diversity maintains liver function in alcohol-associated liver disease
Authors: Sasaki Kyo , Rooge Sheetalnath , Gunewardena Sumedha , Hintz Janice Averilla , Ghosh Priyanka , Pulido Ruiz Isabel Aranzazu , Yuquimpo Kyle , Schonfeld Michael , Mehta Heer , Stevenson Heather L. , Saldarriaga Omar A. , Arroyave Esteban , Tikhanovich Irina , Wozniak Ann L. , Weinman Steven A. 
Published: April 2024

Background and Aims:
Liver macrophages are heterogeneous and play an important role in alcohol-associated liver disease (ALD) but there is limited understanding of the functions of specific macrophage subsets in the disease. We used a Western diet alcohol (WDA) mouse model of ALD to examine the hepatic myeloid cell compartment by single cell RNAseq and targeted KC ablation to understand the diversity and function of liver macrophages in ALD.

Approach and Results:
In the WDA liver, KCs and infiltrating monocytes/macrophages each represented about 50% of the myeloid pool. Five major KC clusters all expressed genes associated with receptor-mediated endocytosis and lipid metabolism, but most were predicted to be noninflammatory and antifibrotic with 1 minor KC cluster having a proinflammatory and extracellular matrix degradation gene signature. Infiltrating monocyte/macrophage clusters, in contrast, were predicted to be proinflammatory and profibrotic. In vivo, diphtheria toxin–based selective KC ablation during alcohol exposure resulted in a liver failure phenotype with increases in PT/INR and bilirubin, loss of differentiated hepatocyte gene expression, and an increase in expression of hepatocyte progenitor markers such as EpCAM, CK7, and Igf2bp3. Gene set enrichment analysis of whole-liver RNAseq from the KC-ablated WDA mice showed a similar pattern as seen in human alcoholic hepatitis.

Conclusions:
In this ALD model, KCs are anti-inflammatory and are critical for the maintenance of hepatocyte differentiation. Infiltrating monocytes/macrophages are largely proinflammatory and contribute more to liver fibrosis. Future targeting of specific macrophage subsets may provide new approaches to the treatment of liver failure and fibrosis in ALD.
Discovering the Potential Value of Coenzyme Q10 as an Adjuvant Treatment in Patients With Depression
Authors: Majmasanaye Mahnam , Mehrpooya Maryam , Amiri Hasan , Eshraghi Azadeh 
Published: April 2024

Abstract

Purpose/Background
Depressive disorder or mental cold is the most common mental disorder, and depression exists all over the world and in all countries and cultures. The results of several studies have shown that using compounds with antioxidant properties has been fruitful in patients with depression. Coenzyme Q10 (CoQ10) is a fat-soluble antioxidant and exerts its antioxidant effect by directly neutralizing free radicals or reducing tocopherol and preventing the inhibition of mitochondrial activity because of oxidative stress. This study aimed to investigate the effects of oral CoQ10 in patients with depression as an adjunctive treatment.

Methods/Procedures
Sixty-nine patients with moderate and severe depression were randomly divided into 2 CoQ10 groups (36) and placebo (33). The first group of patients received CoQ10 supplements at a dose of 200 mg daily for 8 weeks along with standard interventions and treatments for depression, and the second group received standard treatments for depression along with a placebo. The change in the score of Montgomery-Åsberg Depression Rating Scale depression scale was evaluated 4 and 8 weeks after the intervention. Also, at baseline and 8 weeks later at the end of the study, serum levels of total antioxidant capacity, total thiol groups, nitric oxide, malondialdehyde, and interleukin 6 were assessed.

Findings/Results
The changes in the depression score at the end of the study showed that, in the group receiving the CoQ10 supplement after 8 weeks, there was a reduction in depression symptoms, which was statistically significant compared with before the start of the study Meanwhile, no significant changes were observed in the patients of the placebo group in terms of symptom reduction. Compared with baseline and the placebo condition, serum levels of nitric oxide and total thiol groups significantly decreased and increased, respectively. Also, no statistically significant changes were observed for interleukin 6, malondialdehyde, and total antioxidant capacity.

Implications/Conclusions
A dose of 200 mg of CoQ10 supplement daily for 8 weeks can reduce depression and fatigue, as well as improve the quality of life of patients with depression. In addition, CoQ10 can significantly improve inflammation and oxidative stress status in patients with depression.
Symptoms, Lifetime Duration of Estrogen Exposure, and Ovarian Reserve Among Women Living With HIV: A Cross-Sectional Observational Study
Authors: Moore Scott Emory , Davey Christine Horvat , Morgan Michael , Webel Allison 
Published: April 2024

Abstract
This cross-sectional observational study examined associations among symptom burden, lifetime duration of estrogen exposure, and serum antimüllerian hormone (AMH) levels among women living with HIV (n = 98) using bivariate bias-corrected Pearson correlations and multiple correspondence analyses. The mostly Black (85.6%) sample of women, with a mean age of 50 years (SD 12.6 years), exhibited no significant reproductive history factors and symptom burden interrelationships or significant associations between lifetime duration of estrogen exposure and symptoms. Predictably, serum AMH levels were lower among older women; however, less predictable were its significant relationships with months living with HIV (r = −0.362), months on ART (r = −0.270), and CD4+ T-cell nadir (r = 0.347). Symptom–symptom relationships support a fatigue, pain, sleep, anxiety, and depression symptom cluster. The hypotheses were not supported by cross-sectional observation. Further studies should explore variation in relationships between HIV, estrogen exposure, ovarian reserve, and AMH levels over time.
Estimated Clinician-Patient Ratios in US Orthotic and Prosthetic Care: State-Level Trends and Discrepancies
Authors: Ronkos Charles , Mullen Ashley 
Published: April 2024

ABSTRACT

Introduction
For the past 20 years, speculative studies have forewarned of a clinician shortage in the field of orthotics and prosthetics (O&P). Such predictions should emphasize the importance of monitoring potential patient and clinician populations to identify trends and intranational disparities. However, there have been no recent population-based examinations of these previously predicted shortages.

Objectives
This study estimated geographical differences and evaluated national and state-level trends in O&P care within the United States. The study used data on certified O&P clinicians, state populations, and rates of lower-limb amputation secondary to diabetes in Medicare populations.

Study Design
The original research article is a retrospective data analysis.

Methods
Clinician data from the American Board for Certification in Prosthetics Orthotics and Pedorthics, population data from the US Census Bureau, and Medicare data from the Centers for Disease Control and Prevention and the Center for Medicare and Medicaid Services were uploaded into ArcGIS mapping software and geographically weighted for examination across spatial and temporal parameters.

Results
The data show national and state-level trends and disparities. The findings reveal large differences in care environments between states.

Conclusions
Speculation on the ability for current supply to meet perceived demand is complicated, but these data update and elaborate upon previous estimations for predicted clinician scarcity in the near future. Further analysis of state-level distance-to-care discrepancies and an examination of which states would benefit most from expansion of O&P services will be necessary.

Clinical Relevance Statement
This research provides a rudimentary foundation for continued investigation into geographical trends and discrepancies in O&P supply and demand. The successful understanding of these metrics could inform policy and education initiatives, optimize clinical intervention for regional needs, and foster professional expansion into undertreated patient populations.