bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic MIL‐101(Cr)‐NH2 nanocomposite for extraction of nine parabens from chili powder and facial toner using LC–MS/MS
Authors: Zac R Antonia Trisha , Jung Wei‐Ting , Kuo Yen‐Jung , Lee Hui‐Ling 
Published: May 2024

AbstractParabens are frequently used preservatives in food and personal care products. These are also termed endocrine disruptors which have the potential to damage the endocrine system Therefore, an easy and facile extraction and detection of parabens are critically important. This work evaluates different magnetic sorbents such as Fe3O4@MIL‐101(Cr), Fe3O4@MIL‐101(Cr)@NH2, and Fe3O4@MIL‐101(Cr)@SO3H for magnetic solid phase extraction (MSPE) and further selected Fe3O4@MIL‐101(Cr)@NH2 to determine paraben content in chili powder and facial toner samples. Fe3O4@MIL‐101(Cr)@NH2 is an efficient sorbent material specially designed for MSPE, which profoundly extracted nine parabens within a short span of just 6 min, driven by π–π interaction and hydrogen bonding. And a detailed optimization and LC–MS/MS analysis was carried out. Fe3O4@MIL‐101(Cr)@NH2 exhibited superior extraction efficiency, enabling subsequent quantification via LC–MS/MS. The limit of detection ranged from 0.063 to 0.622 ng mL−1 and limit of quantification of 0.211 to 2.073 ng mL−1 were a good recovery in the range of 76.2%–108%. An effective detection of eight parabens in chili powder and four parabens in facial toner samples was determined indicating that Fe3O4@MIL‐101(Cr)‐NH2 sorbents are suitable for MSPE.
Maternal psychological distress and temperament traits in children from infancy to late childhood
Authors: Lahti‐Pulkkinen Marius , Lähdepuro Anna , Lahti Jari , Girchenko Polina , Pyhälä Riikka , Reynolds Rebecca M. , Villa Pia M. , Laivuori Hannele , Kajantie Eero , Heinonen Kati , Räikkönen Katri 
Published: May 2024

AbstractBackgroundMaternal psychological distress during pregnancy is associated with infant temperament. Whether associations persist into late childhood, whether maternal distress is associated with temperament change from infancy to late childhood, whether associations are independent of maternal concurrent distress, and whether maternal distress has sensitive exposure periods on child temperament remain unclear.MethodsOur study includes mother‐child dyads from Finnish, prospective Prediction and Prevention of Preeclampsia and Intrauterine Growth Restriction study. The mothers completed the Center for Epidemiologic Studies Depression Scale, State Anxiety Inventory and Perceived Stress Scale: biweekly up to 14 times during pregnancy; once in infancy (at child age 4–12 months); and once in late childhood (at child age 7–11 years). They also completed the Infant Behavior Questionnaire Revised at the infancy (n = 2538) and Temperament in Middle Childhood Questionnaire at the late childhood (n = 2004; 1693 children had data at both follow‐ups) follow‐up on child negative affectivity, extraversion and effortful control. We examined the associations of maternal distress with child temperament with linear regression, linear mixed and Bayesian relevant lifecourse exposure models.ResultsMaternal distress during pregnancy was associated with higher negative affectivity and lower effortful control in children in infancy and late childhood. Maternal distress during pregnancy was also associated with increases in negative affectivity, decreases in effortful Control, and smaller decreases in extraversion from infancy to late childhood. The associations with late childhood temperament and temperament change were independent of maternal concurrent distress. Late childhood was a sensitive period for lifetime‐to‐date effects of maternal distress on late childhood negative affectivity and effortful control. Distress during pregnancy and infancy had smaller contributions.ConclusionsMaternal psychological distress during pregnancy is associated with individual differences and change in child temperament from infancy to late childhood. However, distress during pregnancy has a smaller effect on late childhood temperament than maternal concurrent distress.
Deregulation of immune response contributing to fulminant hepatitis in HEV infected pregnant women
Authors: Liang Yining , Zhang Jiayue , Luo Dehong , Cheng Lixin , Wang Yijin ,   Chinese Consortium for the Study of Hepatitis E (CCSHE)
Published: May 2024

AbstractHepatitis E virus (HEV) infection in pregnant women is associated with a wide spectrum of adverse consequences for both mother and fetus. The high mortality in this population appears to be associated with hormonal changes and consequent immunological changes. This study conducted an analysis of immune responses in pregnant women infected with HEV manifesting varying severity. Data mining analysis of the GSE79197 was utilized to examine differentially biological functions in pregnant women with HEV infection (P‐HEV) versus without HEV infection (P‐nHEV), P‐HEV progressing to ALF (P‐ALF) versus P‐HEV, and P‐HEV versus non‐pregnant women with HEV infection (nP‐HEV). We found cellular response to interleukin and immune response‐regulating signalings were activated in P‐HEV compared with P‐nHEV. However, there was a significant decrease of immune responses, such as T cell activation, leukocyte cell‐cell adhesion, regulation of lymphocyte activation, and immune response‐regulating signaling pathway in P‐ALF patient than P‐HEV patient. Compared with nP‐HEV, MHC protein complex binding function was inhibited in P‐HEV. Further microRNA enrichment analysis showed that MAPK and T cell receptor signaling pathways were inhibited in P‐HEV compared with nP‐HEV. In summary, immune responses were activated during HEV infection while being suppressed when developing ALF during pregnancy, heightening the importance of immune mediation in the pathogenesis of severe outcome in HEV infected pregnant women.
Risk factors for invasive pulmonary aspergillosis in patients with severe fever with thrombocytopenia syndrome: A multicenter retrospective study
Authors: Yao Lu , Shi Yuan , Fu Jiaji , Fang Xiaowei , Zhang Hongling , Luo Dengli , Zhou Yi , Pan Aijun , Yu Yuan , Yang Xiaobo , Shu Huaqing , Zou Xiaojing , Xu Jiqian , Shang You 
Published: May 2024

AbstractInvasive pulmonary aspergillosis (IPA) is a life‐threatening complication in patients with severe fever with thrombocytopenia syndrome (SFTS), yet SFTS‐associated IPA (SAPA)'s risk factors remain undefined. A multicenter retrospective cohort study across Hubei and Anhui provinces (May 2013–September 2022) utilized least absoluteshrinkage and selection operator (LASSO) regression for variable selection. Multivariable logistic regression identified independent predictors of SAPA, Cox regression highlighted mortality‐related risk factors. Of the 1775 screened SFTS patients, 1650 were included, with 169 developing IPA, leading to a 42‐day mortality rate of 26.6% among SAPA patients. Multivariable logistic regression revealed SAPA risk factors including advanced age, petechia, hemoptysis, tremor, low albumin levels, elongated activated partial thromboplastin time (APTT), intensive care unit (ICU) admission, glucocorticoid usage, intravenous immunoglobulin (IVIG) and prolonged hospital stays. Cox regression identified predictors of 42‐day mortality, including ecchymosis at venipuncture sites, absence of ICU admission, elongated prothrombin time (PT), vasopressor and glucocorticoid use, non‐antifungals. Nomograms constructed on these predictors registered concordance indexes of 0.855 (95% CI: 0.826–0.884) and 0.778 (95% CI: 0.702–0.854) for SAPA onset and 42‐day mortality, respectively. Lower survival rates for SAPA patients treated with glucocorticoids (p < 0.001) and improved 14‐day survival with antifungal therapy (p = 0.036). Improving IPA management in SFTS‐endemic areas is crucial, with effective predictive tool.
IDO‐1 inhibition improves outcome after fluid percussion injury in adult male rats
Authors: Sadek Marawan , Stover Kurt R. , Liu Xiaojing , Reed Mark A. , Weaver Donald F. , Reid Aylin Y. 
Published: May 2024

AbstractThe enzyme indoleamine 2,3 dioxygenase 1 (IDO1) catalyzes the rate‐limiting step in the kynurenine pathway (KP) which produces both neuroprotective and neurotoxic metabolites. Neuroinflammatory signals produced as a result of pathological conditions can increase production of IDO1 and boost its enzymatic capacity. IDO1 and the KP have been implicated in behavioral recovery after human traumatic brain injury (TBI), but their roles in experimental models of TBI are for the most part unknown. We hypothesized there is an increase in KP activity in the fluid percussion injury (FPI) model of TBI, and that administration of an IDO1 inhibitor will improve neurological recovery. In this study, adult male Sprague Dawley rats were subjected to FPI or sham injury and received twice‐daily oral administration of the IDO1 inhibitor PF‐06840003 (100 mg/kg) or vehicle control. FPI resulted in a significant increase in KP activity, as demonstrated by an increased ratio of kynurenine: tryptophan, in the perilesional neocortex and ipsilateral hippocampus 3 days postinjury (DPI), which normalized by 7 DPI. The increase in KP activity was prevented by PF‐06840003. IDO1 inhibition also improved memory performance as assessed in the Barnes maze and anxiety behaviors as assessed in open field testing in the first 28 DPI. These results suggest increased KP activity after FPI may mediate neurological dysfunction, and IDO1 inhibition should be further investigated as a potential therapeutic target to improve recovery.
Creating pragmatic, rapid‐cycle, evidence‐based innovation: The Kaiser Permanente Northern California Delivery Science and Applied Research (DARE) program
Authors: Corley Douglas A. , Schmittdiel Julie A. , Rouillard Smita , Chen Yi‐Fen , Lieu Tracy A. 
Published: May 2024

AbstractIntroductionOngoing crises in the quality, affordability, sustainability, value, and equity of U.S. healthcare call for rapid, massive‐scale innovations across multiple specialties. Physician groups and healthcare organizations commit significant monetary and personnel investments for innovation and improvement efforts, but most lack over‐arching systems theory‐supported conceptual frameworks for efficiently coordinating the timely, large‐volume idea generation, refinement, prioritization, evidence development, implementation, and re‐evaluation strategies needed for rapid‐cycle improvements in health care delivery, outcomes, and value.MethodsThis article describes one large‐scale, generalizable model: The Permanente Medical Group's (TPMG) Delivery Science and Applied Research (DARE) program within Kaiser Permanente Northern California. This organization‐level initiative was designed to: (1) elicit clinical innovation and quality concerns across clinical specialties; (2) prioritize these questions for next‐step evaluations; (3) create dedicated analytic, research, and clinical expertise to rapidly study the questions generated; (4) develop communities of clinician‐researchers, embedded within their specialties' clinical operations, who gather ideas for evaluation, generate evidence, and facilitate implementation of research results (a.k.a. evidence‐based innovators); and (5) broadly disseminate findings, to connect results with potential next‐step implementation.ResultsThe DARE program and its components rapidly led to more than 200 recently completed or ongoing projects, informed care changes, influenced national guidelines, developed communities of evidence‐based clinician innovators in 15 specialties, and empowered new paths for career diversity and physician wellness. Key factors in the DARE program's success include explicitly defining high‐impact ideas; engaging researchers with analysts experienced with large clinical data systems; developing several research funding mechanisms scaled to project size; prioritizing rapid and efficient project completion; supporting clinician‐investigators embedded within their specialties; and structured organization‐wide dissemination of findings for informing potential implementation.ConclusionsHigh‐volume, evidence‐based innovation programs can be important, scale‐able, reproducible models for different settings for increasing quality, affordability, sustainability, value, and equity in healthcare and furthering the difficult‐to‐achieve concept of developing sustainable learning healthcare systems.
Ultra high‐frequency ultrasound of fascicles in the ulnar and radial nerves
Authors: Meiling James B. , Gandhi Mehta Rachana K. , Baute Penry Vanessa , Cartwright Michael S. , McGhee Marisa Barclay 
Published: May 2024

AbstractIntroduction/AimsUltra high‐frequency ultrasound (UHFUS) has been demonstrated to allow easy visualization and quantification of median and digital nerve fascicles; however, there is a lack of normative data for other upper limb nerves. The purpose of this study was to use UHFUS to establish normative reference values and ranges for fascicle count and density within selected upper extremity nerves.MethodsTwenty‐one healthy volunteers underwent sonographic examination of the ulnar, superficial branch of the radial, and radial nerves on one upper limb using UHFUS with a 48 MHz linear transducer. The number of fascicles in each peripheral nerve and fascicle density were assessed.ResultsThe mean fascicle number and fascicle density for each of the measured nerves was ulnar nerve at the wrist 11.7 and 2.0, ulnar nerve at the elbow 9.2 and 1.1, superficial branch of the radial nerve 7.3 and 2.5, and radial nerve at the spiral groove 4.2 and 0.8. A single significant association was observed between CSA and fascicle number in the ulnar nerve at the wrist (p = .023, r = 0.66). Neither fascicle number nor density could be predicted by age, sex, height, weight, or body mass index.DiscussionUHFUS may help to establish a baseline of normative data on upper limb nerves that are not frequently biopsied due to their mixed motor and sensory functions and has the potential for increased understanding of nerve fascicular anatomy to improve diagnostic accuracy of focal nerve lesions, particularly those with selective fascicular involvement.
Discontinuous Galerkin finite element method for dynamic viscoelasticity models of power‐law type
Authors: Jang Yongseok , Shaw Simon 
Published: May 2024

AbstractLinear viscoelasticity can be characterized by a stress relaxation function. We consider a power‐law type stress relaxation to yield a fractional order viscoelasticity model. The governing equation is a Volterra integral problem of the second kind with a weakly singular kernel. We employ spatially discontinuous Galerkin methods, symmetric interior penalty Galerkin method (SIPG) for spatial discretization, and the implicit finite difference schemes in time, Crank–Nicolson method. Further, in order to manage the weak singularity in the Volterra kernel, we use a linear interpolation technique. We present a priori stability and error analyses without relying on Grönwall's inequality, and so provide high quality bounds that do not increase exponentially in time. This indicates that our numerical scheme is well‐suited for long‐time simulations. Despite the limited regularity in time, we establish suboptimal fractional order accuracy in time as well as optimal convergence of SIPG. We carry out numerical experiments with varying regularity of exact solutions to validate our error estimates. Finally, we present numerical simulations based on real material data.
Untargeted metabolomics using UHPLC‐Q‐Orbitrap HRMS for identifying cytotoxic compounds on MCF‐7 breast cancer cells from Annona muricata Linn leaf extracts as potential anticancer agents
Authors: Septaningsih Dewi Anggraini , Suparto Irma Herawati , Achmadi Suminar Setiati , Heryanto Rudi , Rafi Mohamad 
Published: May 2024

AbstractIntroductionThe leaves of Annona muricata L., known as “soursop” or “sirsak” in Indonesia, are used traditionally for cancer treatment. However, the bioactive components remain largely unidentified.ObjectiveThis study used untargeted liquid chromatography–tandem mass spectrometry (LC‐MS/MS)‐based metabolomics to identify potential cytotoxic compounds in A. muricata leaf extracts on MCF‐7 breast cancer cells in vitro.MethodsA. muricata leaves were macerated with water, 99% ethanol, and aqueous mixtures containing 30%, 50%, and 80% ethanol. Cytotoxic activity of the extracts against MCF‐7 breast cancer cells was determined using the MTT assay. Ultra‐high‐performance liquid chromatography–Q‐Orbitrap high‐resolution mass spectroscopy (UHPLC‐Q‐Orbitrap‐HRMS) was used to characterize the metabolite composition of each extract. The correlations between metabolite profile and cytotoxic activities were evaluated using orthogonal partial least square discriminant analysis (OPLS‐DA). The binding of these bioactive compounds to the tumorigenic alpha‐estrogen receptor (3ERT) was then evaluated by in silico docking simulations.ResultsNinety‐nine percent ethanol extracts demonstrated the greatest potency for reducing MCF‐7 cell viability (IC50 = 22 μg/ml). We detected 35 metabolites in ethanol extracts, including alkaloids, flavonoids, and acetogenins. OPLS‐DA predicted that annoreticuin, squadiolin C, and xylopine, and six unknown acetogenin metabolites, might reduce MCF‐7 cell viability. In silico analysis predicted that annoreticuin, squadiolin C, and xylopine bind to 3ERT with an affinity comparable to doxorubicin.ConclusionUntargeted metabolomics and in silico modeling identified cytotoxic compounds on MCF‐7 cells and binding affinity to 3ERT in A. muricata leaf extracts. The findings need to be further verified to prove the screening results.
The incremental yield of prenatal exome sequencing over chromosome microarray for congenital heart abnormalities: A systematic review and meta‐analysis
Authors: Reilly K. , Sonner S. , McCay N. , Rolnik D. L. , Casey F. , Seale A. N. , Watson C. J. , Kan A. , Lai T. H. T. , Chung B. H. Y. , Diderich K. E. M. , Srebniak M. I. , Dempsey E. , Drury S. , Giordano J. , Wapner R. , Kilby M. D. , Chitty L. S. , Mone F. 
Published: May 2024

AbstractObjectivesTo determine the incremental yield of prenatal exome sequencing (PES) over standard testing in fetuses with an isolated congenital heart abnormality (CHA), CHA associated with extra‐cardiac malformations (ECMs) and CHA dependent upon anatomical subclassification.MethodsA systematic review of the literature was performed using MEDLINE, EMBASE, Web of Science and grey literature January 2010‐February 2023. Studies were selected if they included greater than 20 cases of prenatally diagnosed CHA when standard testing (QF‐PCR/chromosome microarray/karyotype) was negative. Pooled incremental yield was determined. PROSPERO CRD 42022364747.ResultsOverall, 21 studies, incorporating 1957 cases were included. The incremental yield of PES (causative pathogenic and likely pathogenic variants) over standard testing was 17.4% (95% CI, 13.5%–21.6%), 9.3% (95% CI, 6.6%–12.3%) and 35.9% (95% CI, 21.0%–52.3%) for all CHAs, isolated CHAs and CHAs associated with ECMs. The subgroup with the greatest yield was complex lesions/heterotaxy; 35.2% (95% CI 9.7%–65.3%). The most common syndrome was Kabuki syndrome (31/256, 12.1%) and most pathogenic variants occurred de novo and in autosomal dominant (monoallelic) disease causing genes (114/224, 50.9%).ConclusionThe likelihood of a monogenic aetiology in fetuses with multi‐system CHAs is high. Clinicians must consider the clinical utility of offering PES in selected isolated cardiac lesions.
Water‐tree aging characteristics of chloroacetic acid allyl ester grafted crosslinked polyethylene
Authors: Ma Fenglian , Zhang Chengcheng , Li Changming , Xu Zhenyu , Zhao Hong 
Published: May 2024

AbstractTo investigate the inhibition effect of chloroacetic acid allyl ester (CAAE) grafting modification on water‐tree aging in crosslinked polyethylene (XLPE), CAAE grafted XLPE (XLPE‐g‐CAAE) with different CAAE contents are prepared by melt blending and grafting modification method. The accelerated water‐tree aging experiments are carried out by the water‐blade electrode method. It is found that XLPE‐g‐CAAE has a great inhibitory effect on the water‐tree aging compared to XLPE, and XLPE‐g‐CAAE with 1.0 phr CAAE has the most significant effect on the water‐tree growth in the scope of the study. The microstructure of XLPE and XLPE‐g‐CAAE is investigated by thermal elongation, gel content, and static water contact angle, respectively. The results show that the grafting of CAAE containing polar groups can enhance the polarity of XLPE and improve the hydrophilicity, which has a dispersing effect on the polar water molecules entering the polymer. Thus, the water molecules are not easy to gather, inhibiting the water‐tree growth. Meanwhile, although the mechanical properties of XLPE‐g‐CAAE decrease to a small extent, and the relative dielectric constant and dielectric loss factor increase slightly, but the performance still meet the requirements of water‐tree resistance XLPE cable insulation.HighlightsCAAE with polar groups was successfully grafted onto XLPE.The polar groups can enhance the polarity of XLPE to improve the hydrophilicity.CAAE grafting can inhibit the water‐tree growth in XLPE.The dielectric properties meet the requirements of water‐tree resistance XLPE.
Influence of structural variations in polysarcosine functionalized lipids on lipid nanoparticle‐mediated mRNA delivery
Authors: Tan Runcheng , Huang Guangqi , Wei Cong , He Zepeng , Zhao Tianyu , Shi Yi , Liu Zhijia , Chen Yongming 
Published: May 2024

AbstractLipid nanoparticles (LNPs) have been demonstrated to be potent and well‐tolerated vehicles for delivering mRNA in vaccination and therapeutics. However, the presence of anti‐poly(ethylene glycol) (PEG) antibodies in the body resulted in the problems of hypersensitivity reaction, accelerated blood clearance and high systemic reactogenicity after repeated dosing of PEG lipid‐contained LNPs, thus limiting the utility for in vivo messenger RNA (mRNA) delivery. Here, we synthesized well‐defined polysarcosine functionalized lipids (pSar‐lipids) with various hydrophobic tail lengths and molecular weights by the accelerated ring‐opening polymerization of sarcosine N‐carboxyanhydride (NCA). The obtained pSar‐lipids were utilized as PEG lipid alternatives to explore structure–activity relationships of pSar‐lipid‐based LNPs. The results demonstrated that pSar‐lipid‐based LNPs by intravenous administration represented higher mRNA delivery efficiency in the liver and spleen with the increased hydrophobic tail length of pSar‐lipids. Importantly, more significant preference for mRNA delivery into the liver was identified by increasing the molecular weight of pSar segments. As a result, this work elucidated the effect of structural variations in pSar‐lipids on LNP‐mediated in vivo mRNA delivery, providing clues to optimize pSar‐lipids as potential alternatives to PEG lipids for developing next‐generation of LNP delivery systems.