bluuebunny/crossref_metadata_2025_split huggingface dataset:
T‐Wave Variability Detects Abnormalities in Ventricular Repolarization: A Prospective Study Comparing Healthy Persons and Olympic Athletes
Authors: Heinz Lara , Sax Anik , Robert Francois , Urhausen Axel , Balta Osman , Kreuz Jens , Nickenig Georg , Ocklenburg Rolf , Schwab Joerg O. 
Published: July 2009

Background: Sudden cardiac death in athletes is more common than in the general population. Routine screening procedures are performed to identify competitors at risk. A new Holter‐based parameter analyzes variation of the ventricular repolarization (TVar). The aim of this study was to evaluate differences in electrocardiogram (ECG), Echo, and Holter (H) in competitive athletes compared to a healthy control group consisting of medical students (MS). Methods: A total of 40 athletes (19 females, Olympic team, Luxembourg) and 40 MS (22 females) were examined by means of a resting ECG, treadmill exercise (TE), echocardiogram (Echo), as well as H recordings during a routine screening visit. To analyze TVar, a 20‐minute H recording at rest (sampling rate 1000 per second) was performed. Moreover, heart rate variability (HRV) as well as HR turbulence (HRT) was computed. Results: No differences in demographic variables were detected. Quantification of HRV detected a significant increase in the vagal component of autonomic cardiac modulation. In contrast, no differences for HRT were found. Echo parameter demonstrated a thicker septal wall without differences of the posterior wall. TVar values were normal in range, but did differ significantly between the two groups. No correlation between TVar and echo as well as Holter parameters was detected. Conclusions: TVar was able to demonstrate significant differences in terms of alterations of ventricular activation. This might indicate an early change of myocardial repolarization representing a substrate for life‐threatening arrhythmia. Larger studies on the predictive value of TVar including follow‐up are necessary to confirm this preliminary finding.
Between love and property: Voice, sentiment, and subjectivity in the reform of daughter's inheritance in Nepal
Authors: KUNREUTHER LAURA 
Published: July 2009

ABSTRACTIn this article, I trace the relation between the figure of voice and subjectivity by examining a Nepali reform movement that sought to give daughters a birth right to ancestral property. At its heart was a contest over emerging class and gender subjectivities that were repeatedly defined through the figure of the voice and related practices of address, hailing, and recognition. The competing formations of voice I discuss here entail shifting notions of intimacy. To challenge property relations thus meant to change existing practices of speech, sentiment, and the meaning of voice itself. [voice, sentiment, property, subjectivity, Nepal, gender, class distinction]
Chthonic science: Georges Niangoran‐Bouah and the anthropology of belonging in Côte d'Ivoire
Authors: ARNAUT KAREL , BLOMMAERT JAN 
Published: July 2009

ABSTRACTGeorges Niangoran‐Bouah worked assiduously toward Africanizing national education, academia, and public culture in Côte d'Ivoire. As part of this venture, his research projects, including his study of “drummology,” can be regarded as a quest for “chthonic” science, that is, an anthropology that uncovers and implements the deep tenets of African–Ivorian culture. Properly situated in its academic, ideological, and political umwelt, we demonstrate, Niangoran‐Bouah's anthropology of belonging is not merely an instance of “closure” but must be seen as a multiform attempt to recover a “local” position as a way to participate in universal–global science. [Niangoran‐Bouah, chthonic science, postcolonial theory, autochthony, Côte d'Ivoire]
One‐year Outcomes Following Coronary Computerized Tomographic Angiography for Evaluation of Emergency Department Patients with Potential Acute Coronary Syndrome
Authors: Hollander Judd E. , Chang Anna Marie , Shofer Frances S. , Collin Mark J. , Walsh Kristy M. , McCusker Christine M. , Baxt William G. , Litt Harold I. 
Published: July 2009

AbstractObjectives:  Coronary computerized tomographic angiography (CTA) has high correlation with cardiac catheterization and has been shown to be safe and cost‐effective when used for rapid evaluation of low‐risk chest pain patients from the emergency department (ED). The long‐term outcome of patients discharged from the ED with negative coronary CTA has not been well studied.Methods:  The authors prospectively evaluated consecutive low‐ to intermediate‐risk patients who received coronary CTA in the ED for evaluation of a potential acute coronary syndrome (ACS). Patients with cocaine use, known cancer, and significant comorbidity reducing life expectancy and those found to have significant disease (stenosis ≥ 50% or ejection fraction < 30%) were excluded. Demographics, medical and cardiac history, labs, and electrocardiogram (ECG) results were collected. Patients were followed by telephone contact and record review for 1 year. The main outcome was 1‐year cardiovascular death or nonfatal acute myocardial infarction (AMI).Results:  Of 588 patients who received coronary CTA in the ED, 481 met study criteria. They had a mean (±SD) age of 46.1 (±8.8) years, 63% were black or African American, and 60% were female. There were 53 patients (11%) rehospitalized and 51 patients (11%) who received further diagnostic testing (stress or catheterization) over the subsequent year. There was one death (0.2%; 95% confidence interval [CI] = 0.01% to 1.15%) with unclear etiology, no AMI (0%; 95% CI = 0 to 0.76%), and no revascularization procedures (0%; 95% CI = 0 to 0.76%) during this time period.Conclusions:  Low‐ to intermediate‐risk patients with a Thrombosis In Myocardial Infarction (TIMI) score of 0 to 2 who present to the ED with potential ACS and have a negative coronary CTA have a very low likelihood of cardiovascular events over the ensuing year.
EORTC trial 11001: distribution of two 10B‐compounds in patients with squamous cell carcinoma of head and neck, a translational research/phase 1 trial
Authors: Wittig Andrea , Collette Laurence , Appelman Klaas , Bührmann Sandra , Jäckel Martin C. , Jöckel Karl‐Heinz , Schmid Kurt Werner , Ortmann Uta , Moss Raymond , Sauerwein Wolfgang A.G. 
Published: July 2009

AbstractBoron neutron capture therapy (BNCT) provides highly targeted delivery of radiation through the limited spatial distribution of its effects. This translational research/phase I clinical trial investigates whether BNCT might be developed as a treatment option for squamous cell carcinoma of head and neck (SCCHN) relying upon preferential uptake of the two compounds, sodium mercaptoundecahydro‐closo‐dodecaborate (BSH) or L‐para‐boronophenylalanine (BPA) in the tumour. Before planned tumour resection, three patients received BSH and three patients received BPA. The 10B‐concentration in tissues and blood was measured with prompt gamma ray spectroscopy. Adverse effects from compounds did not occur. After BPA infusion the 10B‐concentration ratio of tumour/blood was 4.0 ± 1.7. 10B‐concentration ratios of tumour/normal tissue were 1.3 ± 0.5 for skin, 2.1 ± 1.2 for muscle and 1.4 ± 0.01 for mucosa. After BSH infusion the 10B‐concentration ratio of tumour/blood was 1.2 ± 0.4. 10B‐concentration ratios of tumour/normal tissue were 3.6 ± 0.6 for muscle, 2.5 ± 1.0 for lymph nodes, 1.4 ± 0.5 for skin and 1.0 ± 0.3 for mucosa. BPA and BSH deliver 10B to SCCHN to an extent that might allow effective BNCT treatment. Mucosa and skin are the most relevant organs at risk.
CD4/CD8 double negative pagetoid reticulosis: a case report and literature review
Authors: Mourtzinos Nikki , Puri Puja K. , Wang Guanghua , Liu Min‐Ling 
Published: July 2009

Pagetoid reticulosis is an indolent primary cutaneous T‐cell lymphoma. It typically presents as a solitary and slowly growing patch or plaque on the extremity, histologically characterized by an acanthotic epidermis infiltrated with atypical lymphocytes. Here, we present histological, immunophenotypical and molecular findings of a 29‐year‐old Jamaican man with bilateral wrist plaques. Histology showed marked acanthosis, hyperkeratosis and an intraepidermal infiltration consisting of large atypical lymphocytes. Immunohistochemical stains showed CD3 and CD5 positive T cells with significant loss of CD7, double negative CD4 and CD8 and strong positive CD30. Molecular analysis showed a monoclonal T‐cell receptor (TCR) gamma gene rearrangement. Review of the literature confirms that the immunophenotype of pagetoid reticulosis is variable with decreasing frequency of CD8+ cytotoxic/suppressor T cell, CD4+ helper T cell and least commonly CD4/CD8 double negative phenotypes. Although CD4/CD8 double negative phenotype appears to be associated with higher proliferation index, it does not appear to confer prognostic significance.Mourtzinos N, Puri PK, Guanghua W, Liu M‐L. CD4/CD8 double negative pagetoid reticulosis: a case report and literature review.
Intralymphatic atypical T‐cell proliferation in a cutaneous hemangioma
Authors: Ardighieri Laura , Lonardi Silvia , Vermi William , Medicina Daniela , Cerroni Lorenzo , Facchetti Fabio 
Published: July 2009

We report an unusual case of atypical T‐cell proliferation involving the lymphatic vessels within a cutaneous hemangioma from an elderly woman. Despite the blastic morphology, the CD4 restricted phenotype and the very high proliferation index, the clinical presentation (single skin lesion in a healthy woman), the benign clinical course and the absence of T‐cell receptor (TCR) clonal rearrangement favored a reactive nature of the process. Since the atypical cells showed an effector/memory‐like regulatory T‐phenotype (CD45RO+, CD25+ and FOXp3+), expressed the migration‐associated molecule CCR7 and were exclusively located within podoplanin+ lymphatic vessels, we speculate that the process might reflect an unusual local immune response, with migration of T‐cells to draining lymph nodes.Ardighieri L, Lonardi S, Vermi W, Medicina D, Cerroni L, Facchetti F. Intralymphatic atypical T‐cell proliferation arising in a cutaneous hemangioma.
Benign mixed tumor of the skin, hypercellular variant: a case report
Authors: Hafezi‐Bakhtiari Sara , Al‐Habeeb Ayman , Ghazarian Danny 
Published: July 2009

A 71‐year‐old man presented with a slowly growing 2.0×2.0×1.0 cm scalp lesion that was surgically removed. Microscopic examination showed a well‐circumscribed dermally located tumor composed of ductal elements lined by double to multiple cell layers of bland cuboidal inner cells and elongated spindled outer cells with areas showing cribriform and solid growth patterns. Some cells showed prominent cytoplasmic clearing. A few mitotic figures are noted ranging from 1‐2 mitotic figure/10 hpf. There are also foci of squamous differentiation as well as occasional mature adipocytes. The background stroma was predominantly sclerotic with only small area of myxoid background (confirmed by Hale's colloidal iron).Immunohistochemical studies revealed positive immunoreactivity for EMA, CEA, CD117, HWMK, LWMK, CK7, Androgen receptor and S100 in the ductal (epithelial) cells and positive immunereactivity for calponin, SMA, CK 5/6 and p63 in the myoepithelial component. No immunoreactivity for Brst‐2, ER, PR and CK20 was noted. MIB‐1 showed mildly increased proliferrative index highlighting 5% of the nuclei. The overall morphology and immunohistochemical profile are that of a benign cutanoues mixed tumor (chondroid syringoma). Given the unusual striking celluarlity, we suggest to subclassify this as a hyper‐cellular variant.Hafezi‐Bakhtiari S, Al Habeeb A, Ghazarian D. Benign mixed tumor of the skin, hypercellular variant: a case report.
Cytokeratin 75 expression in central, centrifugal, cicatricial alopecia – new observations in normal and diseased hair follicles
Authors: Sperling Leonard C. , Hussey Sean , Sorrells Timothy , Wang Ji‐an , Darling Thomas 
Published: July 2009

Background: Premature desquamation of the inner root sheath (IRS) is an important histological marker for central, centrifugal, cicatricial alopecia (CCCA), and an inherently defective IRS may be responsible. Cytokeratin 75 (K75; formerly K6hf) is an appealing candidate for study because K75 is specifically expressed in the companion layer of the hair follicle, the interface for IRS desquamation. Also, K75 abnormalities have been found in other hair diseases bearing similarities to CCCA. Methods: We used a commercially available antibody to K75 on formalin‐fixed, paraffin‐embedded tissue from clinically and histologically “normal” scalp (n=9); clinically diseased scalp from patients with CCCA (n=15); and clinically “normal” scalp from patients with CCCA (n=6). Results: K75 expression disappears during the process of IRS desquamation, and loss of expression begins even when IRS desquamation is in its incipient phase. Also, K75 has a characteristic pattern of expression in telogen follicles. Conclusions: K75 expression is closely associated with the process of desquamation of the IRS. This process occurs prematurely (below the isthmus) in many follicles from patients with, but not without, CCCA. K75 expression highlights premature desquamation of the IRS in CCCA, but may not be directly involved in disease pathogenesis.Sperling LC, Hussey S, Sorrells T, Wang J‐A, Darling T. Cytokeratin 75 expression in central, centrifugal, cicatricial alopecia – new observations in normal and diseased hair follicles.
Effects of small interfering RNAs targeting Fascin on gene expression in oral cancer cells
Authors: Chen Su‐Feng , Lin Chi‐Yu , Chang Yun‐Ching , Li Jhy‐Wei , Fu Earl , Chang Fei‐Ni , Lin Yu‐Lu , Nieh Shin 
Published: July 2009

Background:  Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. The prognosis of OSCC is usually poor because of extensive local invasion at initial diagnosis. In the literature, Fascin has been reported responsible for cell motility and over‐expression of Fascin contributes to an unfavorable clinical course. Nevertheless, the roles of Fascin protein playing in aggressiveness of OSCC and their potential mechanisms need to be elucidated.Methods:  Two cell lines of OSCC (OECM‐1 and SCC‐25) via the vector‐based small interfering RNA (siRNA) to suppress the expression of the Fascin gene were used. Subsequent analyses and observation regarding the expression of Fascin protein and cyto‐morphological alterations were detected by Western blot and immunofluorescent microscopy. Boyden chamber invasion assay, cell migration assay and adhesion assay were also applied to investigate the functional changes of OSCC.Results:  There were statistically significant differences (P < 0.05) of Fascin expression before and after silencing. Down‐regulation of Fascin protein directly led to changes of cell surface protrusions under immunofluorescent microscopy and resulted in suppression of migration, invasion and increase of adhesion in both cell lines (P < 0.05). Furthermore, down‐regulation of Fascin expression also resulted in alterations of E‐cadherin, β‐catenin and TWIST at certain level, implicative of an association with epithelial‐mesenchymal transition (EMT).Conclusions:  Our results suggest that expression of Fascin protein may play an essential role in regulation of progression of OSCC and contributes to the event of EMT in the early aggressiveness of OSCC.
Inhibition of ameloblastoma invasion in vitro and in vivo by inhibitor of metalloproteinase‐2 activity
Authors: Zhang Bin , Zhang Jin , Huang Hong‐Zhang , Chen Wei‐Liang , Tao Qian , Zeng Dong‐Lin , Zhang Lei‐Tao , Xu Jian‐Hui 
Published: July 2009

Background:  Ameloblastoma is an odontogenic benign tumor characterized by local invasiveness and most of its local recurrences clinically result from local invasion. This study used matrix metalloproteinase‐2 (MMP‐2) inhibitor I (MMP‐2I) to investigate the role played by MMP‐2 activity in the local invasiveness of ameloblastoma.Methods:  The cells and xenografts of ameloblastoma were treated with MMP‐2I and treatment group were compared with the control group. In vitro, the invasive activity of tumor cells was assayed in transwell cell culture chamber. Gelatinolytic activity of gelatinases and MMP‐2/tissue inhibitor of matrix metalloproteinase (TIMP‐2) protein expression was detected using gelatin zymography and flow cytometry. The cell viability and adhesion were evaluated using methyl thiazol tetrazolium. In vivo, bilateral subrenal capsule xenograft transplantation of ameloblastoma was performed in 10 nude mice and the invasion of ameloblastoma into the renal parenchyma was observed.Results:  Active‐MMP‐2 of conditioned media was significantly lower in treatment group than in the control group. Accordingly, potential of in vitro cell invasion, adhesion and in vivo tumor invasion were also significantly lower in the treatment group than in the control group.Conclusions:  Inhibitor of MMP‐2 activity suppressed the local invasive capability of ameloblastoma by decreasing MMP‐2 activity. MMP‐2 activity is in relation with invasive capacity of ameloblastoma.
Degenerative and protective reactions of the rat trigeminal motor nucleus after removal of the masseter and temporal muscles
Authors: Seki Yoshihiro , Suzuki Satoshi O. , Nakamura Seiji , Iwaki Toru 
Published: July 2009

Background:  Microsurgical reconstruction techniques have allowed treatment of advanced head and neck carcinomas; however, it remains difficult to achieve long‐term, functional reconstruction of the faciocervical muscles. To address this issue, in this we developed a rat trigeminal nerve denervation model that closely simulates the effects of oral surgery.Methods:  The rat trigeminal nerve denervation model was developed by removing the masseter and temporal muscles, and degeneration process of the trigeminal motor nucleus was investigated by immunohistochemistry with particular focus on microglial/astrocytic reactions and motoneuron degeneration.Results:  Atrophy of the trigeminal motor nucleus was observed at 8 weeks after denervation. A microglial reaction peaked at 3 days post‐operation, while an astrocytic reaction was evident within 2 weeks, and peaked around 4 weeks post‐operation. Expression of the stress protein HSP27 and an autophagy marker Rab24 was also upregulated in the injured trigeminal motor nucleus.Conclusions:  The results from this study suggest that this model is a practical and useful tool help to develop a further understanding of the pathology of the trigeminal motor nucleus after surgical denervation.
The immunoprofile of odontogenic keratocyst (keratocystic odontogenic tumor) that includes expression of PTCH, SMO, GLI‐1 and bcl‐2 is similar to ameloblastoma but different from odontogenic cysts
Authors: Vered M. , Peleg O. , Taicher S. , Buchner A. 
Published: July 2009

Background:  The aggressive biological behavior of odontogenic keratocysts (OKCs), unlike that of other odontogenic cysts, has argued for its recent re‐classification as a neoplasm, ‘keratocystic odontogenic tumor’. Identification of mutations in the PTCH gene in some of the OKCs that were expected to produce truncated proteins, resulting in loss of control of the cell cycle, provided additional support for OKCs having a neoplastic nature.Methods:  We investigated the immunohistochemical expression of the sonic hedgehog (SHH) signaling pathway‐related proteins, PTCH, smoothened (SMO) and GLI‐1, and of the SHH–induced bcl‐2 oncoprotein in a series of primary OKC (pOKC), recurrent OKC (rOKC) and nevoid basal cell carcinoma syndrome‐associated OKCs (NBCCS‐OKCs), and compared them to solid ameloblastomas (SAMs), unicystic ameloblastomas (UAMs), ‘orthokeratinized’ OKCs (oOKCs), dentigerous cysts (DCs) and radicular cysts (RCs).Results:  All studied lesions expressed the SHH pathway‐related proteins in a similar pattern. The expression of bcl‐2 in OKCs (pOKCs and NBCCS‐OKCs) and SAMs was significantly higher than in oOKCs, DCs and RCs (P < 0.001).Conclusions:  The present results of the immunoprofile of OKCs (that includes the expression of the SHH‐related proteins and the SHH‐induced bcl‐2 oncoprotein) further support the notion of OKC having a neoplastic nature. As OKCs vary considerably in their biologic behavior, it is suggested that the quality and quantity of interactions between the SHH and other cell cycle regulatory pathways are likely to work synergistically to define the individual phenotype and corresponding biological behavior of this lesion.
MYC gene amplification reveals clinical association with head and neck squamous cell carcinoma in Indian patients
Authors: Bhattacharya N. , Roy A. , Roy B. , Roychoudhury S. , Panda C. K. 
Published: July 2009

Background:  Amplification of the MYC gene is reported to be associated with the development of head and neck squamous cell carcinoma (HNSCC). This study is focused to analyze the correlation between MYC gene amplification and various clinicopathological features and outcome in a cohort of 49 dysplastic and 187 primary head and neck lesions.Methods:  MYC gene amplification was assessed by differential polymerase chain reaction using primer sets from the MYC gene as target locus and DRD2 gene as the control locus.Result:  The MYC gene amplification was detected in a total of 23.7% (56/236) head and neck lesions comprising 14.2% (7/49) dysplastic lesions and 26% (49/187) HNSCC samples. The clinicopathological association study between MYC gene amplification with the different clinical parameters like sex, tumor stage, tumor differentiation, lymph node status, tobacco habit and HPV 16/18 status determined significant association of MYC amplification with tumor progression (P = 0.009). Kaplan Meier analysis revealed MYC gene has no prognostic significance on survival in HNSCC.Conclusion:   In conclusion, our results suggest that MYC gene amplification is associated with tumor progression in HNSCC.
Production of proinflammatory and immunoregulatory cytokines by inflammatory cells from periapical lesions in culture
Authors: Gazivoda Dragan , Dzopalic Tanja , Bozic Biljana , Tatomirovic Zeljka , Brkic Zlata , Colic Miodrag 
Published: July 2009

Background:  The role of cytokines in pathogenesis of periapical lesions is not well understood. The aim of this study was to study the correlation between proinflammatory and immunoregulatory cytokines in periapical lesions and their relationship with cellular composition and clinical presentation.Methods:  Inflammatory cells were isolated from 67 human periapical lesions and cultivated for 24 h. The levels of proinflammatory cytokines: interleukin‐1 beta (IL‐1β), IL‐6, IL‐8 and tumour necrosis factor alpha (TNF‐α) and immunoregulatory cytokines: transforming growth factor‐beta (TGF‐β) and IL‐10 were determined in culture supernatants using a fluorescent bead immunoassay or ELISA. The phenotype of cells was analysed by immunocytochemistry.Results:  Inflammatory cells from symptomatic lesions which contained higher proportion of granulocytes, secreted higher levels of IL‐1, IL‐6 and IL‐8 compared with asymptomatic lesions. Large‐size lesions contained lower percentages of mononuclear phagocytes, higher percentages of CD8+ T cells and produced higher levels of TNF‐α, IL‐6 and IL‐10 compared with small‐size lesions. There were negative correlations between the concentrations of TGF‐β and proinflammatory cytokines. TGF‐β, added to cultures, downregulated the levels of proinflammatory cytokines more strongly than IL‐10, independently of clinical presentation of the lesions. By contrast, exogenous IL‐10 was mainly immunosuppressive in cultures of asymptomatic lesions.Conclusion:  Symptomatic lesions are characterized by higher production of proinflammatory cytokines. Immunoregulatory cytokines are more important for suppression of inflammation in asymptomatic lesions and in this context the effect of TGF‐β is more potent and different from IL‐10.