bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymer coating of porcine decellularized and cross‐linked aortic grafts
Authors: Heidenhain Christoph , Weichert Wilko , Schmidmaier Gerhard , Wildemann Britt , Hein Moritz , Neuhaus Peter , Heise Michael 
Published: May 2010

AbstractThis article investigates a method of modifying and optimizing the biocompatibility of decellularized vascular bioimplants when treated with a specialized, drug eluting coating. For this purpose, we carried out aortic transplantations using a porcine model. Decellularized, cross‐linked aortic grafts were coated with poly(D,L‐lactide) (PDLLA). To this coating, we added the anticoagulant drug lepirudin which, following transplantation, would be linearly eluted. These aortic grafts are easily manipulated in surgery. It was shown that, as a result of the lepirudin‐eluting coating, the rate of thrombogenesis was reduced and the patency rate was significantly improved. However, lumen‐stenosing pseudointima developed in all of the transplants and was not effected by PDLLA coating. Furthermore, no evidence of recellularisation was documented. This trial demonstrates that polymer coating of decellularized tissue is possible. Neointimal hyperplasia and the absence of cellular repopulation mark the negative consequences of this concept. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Plerixafor mobilization leads to a lower ratio of CD34+ cells to total nucleated cells which results in greater storage costs
Authors: Tanhehco Yvette C. , Adamski Jill , Sell Mary , Cunningham Kathleen , Eisenmann Christa , Magee Deborah , Stadtmauer Edward A. , O'Doherty Una 
Published: May 2010

AbstractBackground:Plerixafor (Mozobil, AMD3100) with granulocyte‐colony stimulating factor (G‐CSF) mobilizes more CD34+ cells/kg compared to G‐CSF alone. Given that plerixafor enhances mobilization of multiple white blood cell lineages, we determined if more storage space is required for products collected from patients mobilized with plerixafor.Methods:A review of the medical records of 15 patients mobilized with chemotherapy and G‐CSF (control) and 14 patients mobilized with plerixafor plus G‐CSF (plerixafor) was performed. Data on demographics, baseline characteristics, CD34+ cells/kg, total nucleated cells, total mononuclear cells, total apheresis sessions, and total bags for storage were collected. Mean values were determined and compared using Student's t‐test.Results:We found that the proportion of CD34+ cells among total nucleated cells was less in the plerixafor group compared to the control group (P = 0.0427). More nucleated cells (10.7 × 1010 vs. 7.1 × 1010, P =0.0452) and mononuclear cells (9.7 × 1010 vs. 5.9 × 1010, P = 0.0059) were mobilized with plerixafor plus G‐CSF. However, there was no significant difference in CD34+ cells/kg, total CD34+ cells or the proportion of mononuclear cells among total nucleated cells between the two groups. More storage bags were required for the plerixafor group compared to the control group (15 vs. 9, P = 0.0299).Conclusion:Mobilization with plerixafor plus G‐CSF resulted in a smaller proportion of CD34+ cells collected and a greater number of storage bags. An increase in the number of bags required for stem cell storage may be logistically problematic and will also lead to increased costs for storage of stem cells. J. Clin. Apheresis 25:202–208, 2010. © 2010 Wiley‐Liss, Inc.
Computational studies on the mechanism and kinetics of Cl reaction with C2H5I
Authors: Jia Xiu‐Juan , Liu You‐Jun , Sun Jing‐Yu , Sun Hao , Wang Fang , Su Zhong‐Min , Pan Xiu‐Mei , Wang Rong‐Shun 
Published: May 2010

AbstractThe dual‐level direct kinetics method has been used to investigate the multichannel reactions of C2H5I + Cl. Three hydrogen abstraction channels and one displacement process are found for the title reaction. The calculation indicates that the hydrogen abstraction from CH2 group is the dominant reaction channel, and the displacement process may be negligible because of the high barrier. The rate constants for individual reaction channels are calculated by the improved canonical variational transition‐state theory with small‐curvature tunneling correction over the temperature range of 220–1500 K. Our results show that the tunneling correction plays an important role in the rate constant calculation in the low‐temperature range. Agreement between the calculated and experimental data available is good. The Arrhenius expression k(T) = 2.33 × 10−16 T1.83 exp(−185.01/T) over a wide temperature range is obtained. Furthermore, the kinetic isotope effects for the reaction C2H5I + Cl are estimated so as to provide theoretical estimation for future laboratory investigation. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Implementation of an algorithm based on the Runge‐Kutta‐Fehlberg technique and the potential energy as a reaction coordinate to locate intrinsic reaction paths
Authors: Aguilar‐Mogas Antoni , Giménez Xavier , Bofill Josep Maria 
Published: May 2010

AbstractThe intrinsic reaction coordinate (IRC) curve is used widely as a representation of the Reaction Path and can be parameterized taking the potential energy as a reaction coordinate (Aguilar‐Mogas et al., J Chem Phys 2008, 128, 104102). Taking this parameterization and its variational nature, an algorithm is proposed that permits to locate this type of curve joining two points from an arbitrary curve that joints the same initial and final points. The initial and final points are minima of the potential energy surface associated with the geometry of reactants and products of the reaction whose mechanism is under study. The arbitrary curves are moved toward the IRC curve by a Runge‐Kutta‐Fehlberg technique. This technique integrates a set of differential equations resulting from the minimization until value zero of the line integral over the Weierstrass E‐function. The Weierstrass E‐function is related with the second variation in the theory of calculus of variations. The algorithm has been proved in real chemical systems. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
SKATE: A docking program that decouples systematic sampling from scoring
Authors: Feng Jianwen A. , Marshall Garland R. 
Published: May 2010

AbstractSKATE is a docking prototype that decouples systematic sampling from scoring. This novel approach removes any interdependence between sampling and scoring functions to achieve better sampling and, thus, improves docking accuracy. SKATE systematically samples a ligand's conformational, rotational and translational degrees of freedom, as constrained by a receptor pocket, to find sterically allowed poses. Efficient systematic sampling is achieved by pruning the combinatorial tree using aggregate assembly, discriminant analysis, adaptive sampling, radial sampling, and clustering. Because systematic sampling is decoupled from scoring, the poses generated by SKATE can be ranked by any published, or in‐house, scoring function. To test the performance of SKATE, ligands from the Asetex/CDCC set, the Surflex set, and the Vertex set, a total of 266 complexes, were redocked to their respective receptors. The results show that SKATE was able to sample poses within 2 Å RMSD of the native structure for 98, 95, and 98% of the cases in the Astex/CDCC, Surflex, and Vertex sets, respectively. Cross‐docking accuracy of SKATE was also assessed by docking 10 ligands to thymidine kinase and 73 ligands to cyclin‐dependent kinase. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Modeling environmental effects on charge density distributions in polar organometallics: Validation of embedded cluster models for the methyl lithium crystal
Authors: Götz Kathrin , Meier Florian , Gatti Carlo , Burow Asbjörn M. , Sierka Marek , Sauer Joachim , Kaupp Martin 
Published: May 2010

AbstractThe charge density and its Laplacian at the LiC and CH bond critical points and other features of the electron density distribution of the methyl lithium crystal have been compared by density functional methods for (i) the isolated (LiCH3)4tetramer or larger clusters, (ii) for quantum mechanically treated clusters in polarizable continuum model (PCM) surroundings, (iii) for clusters augmented by the periodic electrostatic embedded cluster model (PEECM), and for (iv) the periodic crystal. Comparisons with identical functional and basis sets indicate that both PCM and PEECM embedding of only a tetramer did not fully account for the environmental effect. In contrast, embedding of a full unit cell gave results that were essentially converged to the periodic crystal data. Effects of basis set and exchange correlation functional on the QTAIM bond descriptors are of a comparable order of magnitude as the crystal environmental effects. In this context, embedded cluster computations provide distinct advantages over explicit solid‐state calculations with respect to their freedom of the choice of computational and theoretical level. This is demonstrated by embedded MP2 calculations. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Serum prolidase enzyme activity and its relation to histopathological findings in patients with non‐alcoholic steatohepatitis
Authors: Horoz Mehmet , Aslan Mehmet , Bolukbas Fusun F. , Bolukbas Cengiz , Nazligul Yasar , Celik Hakim , Aksoy Nurten 
Published: May 2010

AbstractThe aim of this study was to investigate serum prolidase enzyme activity and to find out its association with liver biopsy specimens' histopathological findings in patients with nonalcoholic steatohepatitis (NASH), which may progress to liver fibrosis and cirrhosis. Thirty‐six patients with biopsy‐proven NASH and 29 healthy controls were enrolled. Serum prolidase enzyme activity was measured spectrophotometrically. Serum prolidase enzyme activity was significantly higher in patients with NASH thancontrols (P=0.016). A significant correlation was observed between serum prolidase enzyme activity and fibrosis score in patients with NASH (r=0.661,P<0.001). Serum prolidase activity seems to be correlated with the level of fibrosis. Monitoring serum prolidase activity may be a useful adjunctive tool in predicting liver fibrosis, especially in the absence of advanced fibrosis and other conditions, which may affect the interpretation of prolidase activity. J. Clin. Lab. Anal. 24:207–211, 2010. © 2009 Wiley‐Liss, Inc.
Exogenous interferences with Jaffe creatinine assays: addition of sodium dodecyl sulfate to reagent eliminates bilirubin and total protein interference with Jaffe methods
Authors: Srisawasdi P. , Chaichanajarernkul U. , Teerakanjana N. , Vanavanan S. , Kroll M.H. 
Published: May 2010

AbstractBackground: The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic).Methods: Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albumin, glucose, hemoglobin and lipid, and patient sera with maximum concentrations of bilirubin, 1,090 µmol/l and protein, 117.8 g/l.Results: Hemoglobin, 7.5 g/l and lipaemic with triglyceride concentration of 6.27 mmol/l, did not interfere with all assays. Glucose >33.3 mmol/l increased creatinine recovery for Dimension method. Samples spiked with bilirubin imparted a negative bias for Dimension and Architect methods but imparted a positive bias for Vitros assay. However, using patient sera, negative bias with bilirubin was found for all methods, from which Architect method gave the highest effect (R2=0.861), followed by Vitros (R2=0.239) and Dimension (R2=0.163). Protein provided the positive bias for all creatinine measurements that increased with increasing concentration (R2 ranging from 0.104 to 0.182, P<0.0001). Addition of sodium dodecyl sulfate (SDS) in alkaline‐picrate reagent reduced the effect of bilirubin and protein for kinetic Jaffe method. Although adding potassium ferricyanide was well effective for eliminating negative interference of bilirubin, it was prone to interference from protein.Conclusions: Endogenous interferences continue to plague creatinine accuracy measurement in both Jaffe and enzymatic methods, and consequentially the estimated glomerular filtration rate. The addition of SDS to the alkaline‐pirate reagent was shown to be effective in reducing bilirubin and protein interferences. J. Clin. Lab. Anal. 24:123–133, 2010. © 2009 Wiley‐Liss, Inc.
Evaluation of the poly(ADP‐ribose) polymerase‐1 gene variants in Alzheimer's disease
Authors: Liu Hsin‐Ping , Lin Wei‐Yong , Wu Bor‐Tsang , Liu Shu‐Hsiang , Wang Wen‐Fu , Tsai Chon‐Haw , Lee Chun‐Cheng , Tsai Fuu‐Jen 
Published: May 2010

AbstractAmyloid peptide is thought to play a critical role in neuronal death in Alzheimer's disease (AD), most likely through oxidative stress. Free radical‐related injury leads to DNA breaks, which subsequently activates the repair enzyme poly(ADP‐ribose) polymerase‐1 (PARP‐1). In this study, the relationship between genetic variants situated at the PARP‐1 gene and AD development was investigated. We performed a case and control study from a Taiwanese population enrolled 120 AD patients and 111 healthy controls by using a polymerase chain reaction restriction fragment length polymorphism approach for two PARP‐1 exonic polymorphisms, 414C/T (rs1805404) and 2456T/C (rs1136410), corresponding to protein residues at positions 81Asp/Asp and762Val/Ala. There were no significant differences in allele or genotype frequencies for either PARP‐1 gene variant between the case and control groups; however, upon analysis of the haplotype distribution, four haplotypes (Hts) were identified. We found that the distributions of Ht3‐TT and Ht4‐CC were significantly associated with an increased risk of AD (P<0.0001), whereas the Ht1‐TC haplotype showed a protective effect for cases compared with the control group (P<0.05). These results reveal that the PARP‐1 gene is highly associated with AD susceptibility and might contribute to a critical mechanism that mediates cell survival or death as a response to cytotoxic stress. J. Clin. Lab. Anal. 24:182–186, 2010. © 2010 Wiley‐Liss, Inc.
Application of monoclonal antibodies to measure metabolism of an anti‐trypanosomal compound in vitro and in vivo
Authors: Goldsmith Rachel Beaulieu , Gray Danny R. , Yan Zhixia , Generaux Claudia N. , Tidwell Richard R. , Reisner Howard M. 
Published: May 2010

AbstractHuman African trypanosomiasis (HAT), also called African sleeping sickness, is a neglected tropical parasitic disease indigenous to sub‐Saharan Africa. Diamidine compounds, including pentamidine and CPD‐0801, are potent anti‐trypanosomal molecules. The latter is a potential drug in the development at the UNC based Consortium for Parasitic Drug Development. An orally bioavailable prodrug of CPD‐0801, DB868, is metabolized primarily in the liver to the active form. A monoclonal antibody developed against a pentamidine derivative has shown significant reactivity with CPD‐0801 (EC50 65.1 nM), but not with the prodrug (EC50>18,000 nM). An inhibitory enzyme‐linked immunosorbent assay (IELISA) has been used to quantitatively monitor prodrug metabolism by detecting the production of the active compound over time in a sandwich culture rat hepatocyte system and in rats. These results were compared with the results of the standard LC/MS/MS assay. Spearman coefficients of 0.96 and 0.933 (in vitro and in vivo, respectively) indicate a high correlation between these two measurement methods. This novel IELISA provides a facile, inexpensive, and accurate method for drug detection that may aide in elucidating the mechanisms of action and toxicity of existing and future diamidine compounds. J. Clin. Lab. Anal. 24:187–194, 2010. © 2010 Wiley‐Liss, Inc.
Microvasculature of the buffalo (Bubalus bubalis) choroid plexuses: Structural, histochemical, and immunocytochemical study
Authors: Scala Gaetano , Corona Mario , Langella Emilia , Maruccio Lucianna 
Published: May 2010

AbstractThe choroid plexuses (CPs) in mammals produce the cerebrospinal fluid (CSF). In the literature, the morphology of CPs and the process that regulates the production of CSF are virtually nonexistent for domestic ruminants. Thus this study has two aims: 1. to investigate the morpho‐structure of the buffalo CP microvasculature utilizing light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques, and 2. to investigate the relationship between the blood vessels and both the elongated cells and the cells with multiple protrusions located in the CPs. SEM and TEM analyses of the CPs from buffalo brain showed morphological and structural features similar those reported in other mammalian species. Moreover the blood microvasculature is the major component responsible for the formation of the CSF, secreted by the encephalic CPs. In addition the chemical composition of this fluid depends on several morpho‐functional characteristics of the vascularization of the CPs. These characteristics are as follows: two shapes of the vascular organization: lamina‐like and ovoid‐like elongated cells of the CPs, which connect the ventricular cavities to the blood capillaries; and the CP capillaries have diverse forms. In the present study the employment of NADPHd and NOS I was taken as indirect evidence for the presence of NO for investigation their specific role in CPs. Then NOS I immunoreactivity is found in the walls of CP blood vessels demonstrating indirectly the presence of NO with a vaso‐dilatatory and autoregulation function of vascular tone by cholinergic nerve stimulation of blood vessel smooth muscle. Microsc. Res. Tech. 74:67‐75, 2011. © 2010 Wiley‐Liss, Inc.
Secretory profile of metapleural gland cells of the leaf‐cutting ant Acromyrmex coronatus (Formicidae: Attini)
Authors: Vieira Alexsandro Santana , Bueno Odair Correa , Camargo‐Mathias Maria Izabel 
Published: May 2010

AbstractAnts present a pair of metapleural glands located at the posterolateral end of the thorax. Because of its importance in the social organization of ants, the present study was aimed at describing the morphophysiology of this gland in three worker castes of Acromyrmex coronatus, focused on secretory activity using histological and histochemical techniques. Our findings revealed that the secretory and the storage portions of this gland are connected by extracytoplasmic portion of canaliculi that drain the secretion from each secretory cell to the collecting chamber. This secretion contains glycoproteins. In minor workers, the secretion contains higher levels of polysaccharides when compared to that of major workers, supporting the role of the metapleural gland in the maintenance of the fungus garden. The nucleus as well as cytoplasm of secretory cells were strongly positive for RNA indicating that these cells are active in the synthesis of proteins and lipids, compounds found in the final secretion. The variant of the CEC revealed that the secretory activity of the entire gland is synchronous, as all cells exhibit the result. Microsc. Res. Tech. 74:76‐83, 2011. © 2010 Wiley‐Liss, Inc.
Gene therapy for psoriasis in the K14‐VEGF transgenic mouse model by topical transdermal delivery of interleukin‐4 using ultradeformable cationic liposome
Authors: Li Jiong , Li Xia , Zhang Yan , Zhou Xi K. , Yang Han S. , Chen Xian C. , Wang Yong S. , Wei Yu Q. , Chen Li J. , Hu Huo Z. , Liu Chang Y. 
Published: May 2010

AbstractBackgroundTopical transdermal gene delivery to the skin shows great potential for painless, non‐invasive administration of vaccines and therapeutic agents. Interleukin (IL)‐4 strategies have shown a good antipsoriatic effect in clinic trials. To date, no information has been acquired on the effectiveness of gene therapy for psoriasis in the K14‐VEGF transgenic mouse model by topical transdermal penetration of murine IL‐4 (mIL‐4) using ultradeformable cationic liposome (UCL).MethodsIn the present study, we synthesized an UCL and determined a suitable formula for transdermally delivering plasmid DNA to mouse skin. We then tested the antipsoriatic efficacy in the K14‐VEGF transgenic mouse model by transdermal delivery of mIL‐4 using UCL.ResultsWe found that plasmid DNA was transdermally delivered to vicinal sites of epidermis and hair follicles using this optimized formula. Plasmid DNA expression was detected in ear skin. Twenty‐four hours after topical application, plasmid DNA was not detected in blood serum and liver, which may decrease the risk of insertion of promoter from plasmid to genomic DNA. Mice treated with UCL/mIL‐4 displayed a mild psoriasis phenotype. Histological analysis of pathological score using the Baker scoring system revealed an antipsoriatic effect. Immunohistochemical analysis revealed that hyperplastic and inflamed vessels were suppressed.ConclusionsThese observations provide evidence of antipsoriatic efficacy by topical transdermal delivery of mIL‐4. Therefore, topical transdermal gene transfer is attractive and offers future potential for application in human patients with other dermatogic diseases. Copyright © 2010 John Wiley & Sons, Ltd.
The vilsmeier‐haack reaction. IV. Reaction of phosphorus oxychloridedimethylformamide with semicarbazones
Authors: Kira M. A. , Aboul‐Enein M. N. , Korkor M. I. 
Published: May 2010

AbstractKetone semicarbazones react with two moles of phosphorus oxychloride‐dimethylformamide with the formation of 3‐substituted pyrazole‐4‐carboxaldehydes. Aldehyde semicarbazones give, with one mole of phosphorus oxychloride‐dimethylformamide, amidazines.
10th international symposium on the synthesis and applications of isotopes and isotopically labelled compounds—the role of isotopes in pharmacokinetics and drug metabolism Session 15, Wednesday, June 17, 2009
Authors: Braun Matt , Fontana Erminia 
Published: May 2010

AbstractConducting ADME studies on new drug candidates remains one of the primary uses of isotopically labelled compounds. In this session, the synthesis of isotopically labelled (2H, 3H, and 14C) compounds for this purpose has been discussed. Coupling the use of AMS and isotopically labelled compounds for studies in neonatal research and care is also highlighted. Copyright © 2010 John Wiley & Sons, Ltd.
10th international symposium on the synthesis and applications of isotopes and isotopically labelled compounds—production of isotopes, Session 14, Thursday, June 18, 2009
Authors: Brown Darren , Greene John 
Published: May 2010

AbstractThe theme of this session was the production of isotopes. Papers detailing the production of both stable and radioactive isotopes were detailed. Copyright © 2010 John Wiley & Sons, Ltd.
Electrical model of organic diodes with field‐dependent carrier mobility in the presence of an electric field at the injection interface
Authors: Alvarez A. L. , Romero B. , Arredondo B. , Quintana X. , Mallavia R. , Otón J. M. 
Published: May 2010

AbstractIn this study, we propose a model to simulate electrical conduction of single carrier organic diodes in the presence of a barrier for carrier injection and considering a field‐dependent carrier mobility. An analytical expression for the internal electric field function, that simplifies operation with the resulting nonlinear equation system, is provided. Simulated results with this model are compared with those obtained from other existing approaches that assume simplifications. Current density versus voltage curves predicted by this model for diodes with different active layer thicknesses show good agreement with the experimental results. Copyright © 2010 John Wiley & Sons, Ltd.