bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing the effectiveness of targeted agents in adjuvant therapy for patients with metastatic colorectal cancer undergoing surgical resection: a retrospective cohort study
Authors: Su Yi-Chia , Wu Chih-Chien , Chen Yu-Hsun , Su Chien-Chou , Chang Yu-Ching , Hsieh Meng-Che , Kao Yang Yea-Huei 
Published: April 2024

Background: Primary tumor resection and metastasectomy may be beneficial for many patients with metastatic colorectal cancer (mCRC). Objective: To assess the differences in postoperative survival outcomes between adjuvant therapy with chemotherapy alone and chemotherapy plus targeted agents (TAs). Design: Retrospective cohort study. Methods: Patients with mCRC who underwent surgical resection for primary colorectal tumor and distant metastases and received adjuvant therapy from 1 January 2010 to 31 December 2017 were enrolled in the Taiwan Cancer Registry. We analyzed the overall survival of patients with resectable or initially unresectable mCRC who received adjuvant chemotherapy alone and chemotherapy plus TAs. Results: We enrolled 1124 and 542 patients with resectable and initially unresectable mCRC, respectively. Adjuvant chemotherapy plus TAs and chemotherapy alone resulted in similar mortality rates among patients with resectable mCRC [adjusted hazard ratio (aHR) = 1.13; 95% confidence interval (CI), 0.93–1.36]; however, it marginally reduced the mortality rate among patients with initially unresectable mCRC who underwent conversion surgery after neoadjuvant therapy (aHR = 0.81; 95% CI, 0.62–1.06). The subgroup analysis of patients who received more than nine cycles of TAs preoperatively and anti-epidermal growth factor receptor agents revealed aHRs of 0.48 (95% CI, 0.27–0.87) and 0.33 (95% CI, 0.18–0.60), respectively. Conclusion: Adjuvant chemotherapy plus TAs may improve survival in patients with initially unresectable tumors who underwent conversion surgery following neoadjuvant therapy with TAs, especially in those who respond well to the targeted therapy. Our study underscores the importance of stratifying patients with mCRC based on tumor resectability when selecting the adjuvant therapy regimen.
Ocimum basilicum and Ocimum americanum: A Systematic Literature Review on Chemical Compositions and Antimicrobial Properties
Authors: Wirtu Shuma Fayera , Mishra Ashutosh Kumar , Jule Leta Tesfaye , Ramaswamy Krishnaraj 
Published: April 2024

Objectives This systematic review aimed to evaluate the chemical compositions and Pharmacological applications of Ocimum basilicum, known as sweet basil, and Ocimum americanum, known as hoary basil, to assess their antimicrobial properties. Both plants are renowned for their culinary uses and medicinal properties, with studies suggesting potential therapeutic benefits due to their rich essential oil. Methods A systematic approach was adopted to gather and analyze the relevant literature on the chemical profiles and pharmacological activities of Ocimum basilicum and Ocimum americanum. Electronic databases including Web of Science, ScienceDirect, Google Scholar, and PubMed were searched using keywords such as chemical composition of Ocimum basilicum, chemical composition of Ocimum americanum, antimicrobial activities of Ocimum basilicum, and antimicrobial activities of Ocimum americanum. Results Ninety-nine articles meeting the determined inclusion criteria were incorporated in this systematic review. Several studies have consistently demonstrated the antimicrobial activities of these 2 plants, although the specific compounds responsible for this activity vary between the 2 species. Conclusion The diverse array of bioactive molecules present in these plants contributes to their pharmacological effects, suggesting avenues for further research into their therapeutic applications. This review supports the continued exploration of the medicinal properties and potential development of sweet basil and hoary basil as antimicrobial agents.
Knee Cartilage Injuries in Football Players: Clinical Outcomes and Return to Sport After Surgical Treatment: A Systematic Review of the Literature
Authors: Andriolo Luca , Marín Fermín Theodorakys , Chiari Gaggia Giulia Marcella Maryse , Serner Andreas , Kon Elizaveta , Papakostas Emmanuel , Massey Andrew , Verdonk Peter , Filardo Giuseppe 
Published: April 2024

Objective To systematically review the literature and analyze clinical outcomes and return-to-sport after surgical management of cartilage injuries in football players. Design A systematic literature review was performed in August 2023 on PubMed, WebOfScience, and Cochrane Library to collect studies on surgical strategies for cartilage lesions in football players. Methodological quality and risk of bias were assessed with the modified Coleman Methodology score and RoB2 and RoBANS2 tools. Results Fifteen studies on 409 football players (86% men, 14% women) were included: nine prospective and two retrospective case series, one randomized controlled trial, one prospective comparative study, one case report, and one survey. Bone marrow stimulation (BMS) techniques were the most documented. The lesion size influenced the treatment choice: debridement was used for small lesions (1.1 cm2), BMS, osteochondral autograft transplantation (OAT), matrix-assisted autologous chondrocytes transplantation (MACT), and scaffold-augmented BMS for small/mid-size lesions (2.2-3.0 cm2), and autologous chondrocytes implantation (ACI) for larger lesions (5.8 cm2). The surgical options yielded different results in terms of clinical outcome and return-to-sport, with fastest recovery for debridement and scaffold-augmented BMS. The current evidence is limited with large methodological quality variation (modified Coleman Methodology score 43.5/100) and a high risk of bias. Conclusions Decision-making in cartilage injuries seems to privilege early return-to-sport, making debridement and microfractures the most used techniques. The lesion size influences the treatment choice. However, the current evidence is limited. Further studies are needed to confirm these findings and establish a case-based approach to treat cartilage injuries in football players based on the specific patient and lesion characteristics and the treatments’ potential in terms of both return-to-sport and long-term results. Level of evidence Systematic review, level IV.
Cetuximab for the treatment of cutaneous squamous-cell carcinoma in kidney transplant recipients – A retrospective cohort study
Authors: Nobel Hila , Icht Oded , Ben Dor Naomi , Popovtzer Aron , Gitter Limor , Davidovici Batya , Rahamimov Ruth , Kurman Noga 
Published: April 2024

Background: Kidney transplant recipients are at high risk of developing cutaneous squamous cell carcinoma (cSCC) due to prolonged immunosuppression. While most lesions can be managed with local therapy, a subset of patients develop advanced cSCC that is unresectable, recurrent or metastatic. Immune check point inhibitors (ICI) and chemotherapy may jeopardize the transplanted kidney. Cetuximab, an anti-epidermal growth factor receptor antibody, has demonstrated efficacy in advanced cSCC, but data regarding efficacy and safety in solid organ transplant recipients is lacking. Methods: This retrospective cohort study analyzed kidney transplant recipients diagnosed with locally advanced or metastatic cSCC who were treated with cetuximab. Results: Seventeen patients were included in the analysis. The median age was 57 years at the time of kidney transplantation and 62 years at diagnosis of index lesion. Cetuximab was administered with radiation therapy as a radiosensitizer in 58.8% of patients, as monotherapy in 17.6% of patients, and with chemotherapy in 23.5% of patients. Importantly, no detrimental impacts on kidney graft function were observed. The main toxicites were grade 1–2 skin reactions and electrolyte imbalance. Of the 10 patients receiving cetuximab with chemoradiotherapy as a radiosesnitizer, 2 were treated with definitive intent, 7 of them in the adjuvantsetting, and 1 for palliative pourpose. Over half of these patients achieved a complete response (37.29%) or no recurrence after resection (23.5%). Median time to disease progression was 10 months. Conclusions: This novel study suggests cetuximab has a reasonable safety profile and potential efficacy in kidney transplant recipients with advanced cSCC, without impairing allograft function. Further prospective research with larger sample sizes is warranted to confirm these findings. Cetuximab should be investigated as a systemic treatment option for this high-risk population of solid organ transplant recipientss with recurrent, metastatic, or unresectable cSCC.
Combined ACL and Anterolateral Ligament Reconstruction Using an Adjustable-Loop Fixation Device: Surgical Technique
Authors: Jaramillo Quiceno German Alejandro , Sarmiento Riveros Paula Andrea , Helito Camilo Partezani , Arias Perez Ruben Dario , Giardino Moreira da Silva Andre 
Published: April 2024

Background: There are several techniques for the combined reconstruction of the anterior cruciate ligament (ACL) and the anterolateral ligament (ALL), but none have shown superiority. This study aims to present a surgical technique that theoretically reduces some of the risks reported in these procedures. Indications: The main indications for the ALL reconstruction include chronic ACL injuries, revision ACL reconstruction, acute ACL injuries in patients with high-grade pivot-shift (grades 2 or 3), patients involved in pivoting sports, and generalized ligament hyperlaxity or knee hyperextension. Technique Description: Hamstring tendon autografts are used for the ACL and ALL reconstruction; a common femoral ACL and ALL tunnel is made outside-in, making a short socket. A continuous looped hamstring tendon autograft attached to one button is used. The ACL is fixed with an adjustable-loop button. Through small incisions, the tibial fixation of both grafts is performed with bioabsorbable screws. Results: We present a 19-year-old professional soccer player with an acute ACL tear, with no associated meniscal or chondral lesions. Owing to his young age and high-risk sports practice, a combined ACL and ALL reconstruction was performed. The combined ACL and ALL reconstruction with a single femoral tunnel using the hamstrings tendons graft is a well-established procedure for ACL augmentation, reducing failure rates for certain risk groups, with excellent outcomes reported. The use of an adjustable-loop button on the femur is a viable option for this kind of reconstruction with postoperative stability comparable to the interference screw, classically used for this kind of reconstruction. Discussion/Conclusion: This technique has some advantages, such as the use of a short outside-in femoral socket, thus theoretically improving healing. In addition, this lowers the risk of lateral collateral ligament injury, and since the ileotibial band graft is not used, the risk of injuring the lateral inferior genicular vessels and the peroneal nerve is theoretically reduced. Besides, using an adjustable-loop cortical button in the femur can theoretically reduce potential complications such as screw migration, soft-tissue impingement, femoral-site pain, and implant removal. Finally, the reconstruction is done with small incisions, improving the aesthetic result and postoperative pain. Considering all the aforementioned factors, this technique theoretically offers some benefits. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
The Relationship Between Employment Status and Prior Education to Academic and Clinical Success for Diagnostic Medical Sonography or Radiography Students
Authors: Miller Elena 
Published: April 2024

Objective: It has been reported that employment during student enrollment, in rigorous health care programs, impedes college success and may be a barrier to successful graduation. Furthermore, student’s prior education is typically viewed as a positive predictor of success in college. The aim of this study was to examine whether a relationship exists between employment status and prior education, compared with academic and clinical success for Diagnostic Medical Sonography (DMS) or Radiography (RT) students, in a large community college. Materials and Methods: There were 26 imaging student participants consented to this study. This convenient sample included 10 DMS and 16 RT students, enrolled in an associate degree in applied sciences program. A survey was administered that collected demographic data on the students in both programs. Data collection began at the start of each respective program and again one year later. Student academic and clinical grades were examined over a period of three college semesters. Results: The analysis of the findings did not show a significant relationship between employment status, prior education, student academics, and clinical success among the participating DMS or RT students. Conclusion: The results of this study provide limited evidence that there was no significant relationship between employment status, prior education, and academic and clinical success among this cohort of DMS or RT students. These findings also suggest that students’ employment did not impede success for these DMS and RT students. In addition, this research shows that students’ prior education had no effect on their success in their DMS and RT programs. Further research should be conducted to investigate whether these findings are similar in other imaging programs.
Evolution of bird sex chromosomes: a cytogenomic approach in Palaeognathae species
Authors: Setti Príncia Grejo , Deon Geize Aparecida , Zeni dos Santos Rodrigo , Goes Caio Augusto Gomes , Garnero Analía Del Valle , Gunski Ricardo José , de Oliveira Edivaldo Herculano Corrêa , Porto-Foresti Fábio , de Freitas Thales Renato Ochotorena , Silva Fábio Augusto Oliveira , Liehr Thomas , Utsunomia Ricardo , Kretschmer Rafael , de Bello Cioffi Marcelo 
Published: April 2024

Abstract
Background
Different patterns of sex chromosome differentiation are seen in Palaeognathae birds, a lineage that includes the ratites (Struthioniformes, Rheiformes, Apterygiformes, Casuariiformes, and the sister group Tinamiformes). While some Tinamiform species have well-differentiated W chromosomes, both Z and W of all the flightless ratites are still morphologically undifferentiated. Here, we conducted a comprehensive analysis of the ZW differentiation in birds using a combination of cytogenetic, genomic, and bioinformatic approaches. The whole set of satDNAs from the emu (Dromaius novaehollandiae) was described and characterized. Furthermore, we examined the in situ locations of these satDNAs alongside several microsatellite repeats and carried out Comparative Genomic Hybridizations in two related species: the greater rhea (Rhea americana) and the tataupa tinamou (Crypturellus tataupa).

Results
From the 24 satDNA families identified (which represent the greatest diversity of satDNAs ever uncovered in any bird species), only three of them were found to accumulate on the emu’s sex chromosomes, with no discernible accumulation observed on the W chromosome. The W chromosomes of both the greater rhea and the emu did not exhibit a significant buildup of either C-positive heterochromatin or repetitive DNAs, indicating their large undifferentiation both at morphological and molecular levels. In contrast, the tataupa tinamou has a highly differentiated W chromosome that accumulates several DNA repeats.

Conclusion
The findings provide new information on the architecture of the avian genome and an inside look at the starting points of sex chromosome differentiation in birds.
Integrated analysis of transcriptome and miRNAome reveals the heat stress response of Pinellia ternata seedlings
Authors: Bo Chen , Liu Mengmeng , You Qian , Liu Xiao , Zhu Yanfang , Duan Yongbo , Wang Dexin , Xue Tao , Xue Jianping 
Published: April 2024

AbstractPinellia ternata (Thunb.) Briet., a valuable herb native to China, is susceptible to the “sprout tumble” phenomenon because of high temperatures, resulting in a significant yield reduction. However, the molecular regulatory mechanisms underlying the response of P. ternata to heat stress are not well understood. In this study, we integrated transcriptome and miRNAome sequencing to identify heat-response genes, microRNAs (miRNAs), and key miRNA-target pairs in P. ternata that differed between heat-stress and room-temperature conditions. Transcriptome analysis revealed extensive reprogramming of 4,960 genes across various categories, predominantly associated with cellular and metabolic processes, responses to stimuli, biological regulation, cell parts, organelles, membranes, and catalytic and binding activities. miRNAome sequencing identified 1,597 known/conserved miRNAs that were differentially expressed between the two test conditions. According to the analysis, genes and miRNAs associated with the regulation of transcription, DNA template, transcription factor activity, and sequence-specific DNA binding pathways may play a major role in the resistance to heat stress in P. ternata. Integrated analysis of the transcriptome and miRNAome expression data revealed 41 high-confidence miRNA-mRNA pairs, forming 25 modules. MYB-like proteins and calcium-responsive transcription coactivators may play an integral role in heat-stress resistance in P. ternata. Additionally, the candidate genes and miRNAs were subjected to quantitative real-time polymerase chain reaction to validate their expression patterns. These results offer a foundation for future studies exploring the mechanisms and critical genes involved in heat-stress resistance in P. ternata.
Genome-wide identification and evolutionary analysis of the NRAMP gene family in the AC genomes of Brassica species
Authors: Zhao Yuquan , Xie Qijun , Yang Qian , Cui Jiamin , Tan Wenqing , Zhang Dawei , Xiang Jianhua , Deng Lichao , Guo Yiming , Li Mei , Liu Lili , Yan Mingli 
Published: April 2024

Abstract
Background
Brassica napus, a hybrid resulting from the crossing of Brassica rapa and Brassica oleracea, is one of the most important oil crops. Despite its significance, B. napus productivity faces substantial challenges due to heavy metal stress, especially in response to cadmium (Cd), which poses a significant threat among heavy metals. Natural resistance-associated macrophage proteins (NRAMPs) play pivotal roles in Cd uptake and transport within plants. However, our understanding of the role of BnNRAMPs in B. napus is limited. Thus, this study aimed to conduct genome-wide identification and bioinformatics analysis of three Brassica species: B. napus, B. rapa, and B. oleracea.

Results
A total of 37 NRAMPs were identified across the three Brassica species and classified into two distinct subfamilies based on evolutionary relationships. Conservative motif analysis revealed that motif 6 and motif 8 might significantly contribute to the differentiation between subfamily I and subfamily II within Brassica species. Evolutionary analyses and chromosome mapping revealed a reduction in the NRAMP gene family during B. napus evolutionary history, resulting in the loss of an orthologous gene derived from BoNRAMP3.2. Cis-acting element analysis suggested potential regulation of the NRAMP gene family by specific plant hormones, such as abscisic acid (ABA) and methyl jasmonate (MeJA). However, gene expression pattern analyses under hormonal or stress treatments indicated limited responsiveness of the NRAMP gene family to these treatments, warranting further experimental validation. Under Cd stress in B. napus, expression pattern analysis of the NRAMP gene family revealed a decrease in the expression levels of most BnNRAMP genes with increasing Cd concentrations. Notably, BnNRAMP5.1/5.2 exhibited a unique response pattern, being stimulated at low Cd concentrations and inhibited at high Cd concentrations, suggesting potential response mechanisms distinct from those of other NRAMP genes.

Conclusions
In summary, this study indicates complex molecular dynamics within the NRAMP gene family under Cd stress, suggesting potential applications in enhancing plant resilience, particularly against Cd. The findings also offer valuable insights for further understanding the functionality and regulatory mechanisms of the NRAMP gene family.
Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton
Authors: Li Xiaotong , Gu Yunqi , Kayoumu Mirezhatijiang , Muhammad Noor , Wang Xiangru , Gui Huiping , Luo Tong , Wang Qianqian , Wumaierjiang Xieraili , Ruan Sijia , Iqbal Asif , Zhang Xiling , Song Meizhen , Dong Qiang 
Published: April 2024

AbstractThe enzyme glutamine synthetase (GLN) is mainly responsible for the assimilation and reassimilation of nitrogen (N) in higher plants. Although the GLN gene has been identified in various plants, there is little information about the GLN family in cotton (Gossypium spp.). To elucidate the roles of GLN genes in cotton, we systematically investigated and characterized the GLN gene family across four cotton species (G. raimondii, G. arboreum, G. hirsutum, and G. barbadense). Our analysis encompassed analysis of members, gene structure, cis-element, intragenomic duplication, and exploration of collinear relationships. Gene duplication analysis indicated that segmental duplication was the primary driving force for the expansion of the GhGLN gene family. Transcriptomic and quantitative real-time reverse-transcription PCR (qRT-PCR) analyses indicated that the GhGLN1.1a gene is responsive to N induction treatment and several abiotic stresses. The results of virus-induced gene silencing revealed that the accumulation and N use efficiency (NUE) of cotton were affected by the inactivation of GhGLN1.1a. This study comprehensively analyzed the GhGLN genes in Gossypium spp., and provides a new perspective on the functional roles of GhGLN1.1a in regulating NUE in cotton.