bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymerization of insulin‐like growth factor‐binding protein‐1 (IGFBP‐1) potentiates IGF‐I actions in placenta
Authors: Shibuya Hiromi , Sakai Keiji , Kabir‐Salmani Maryam , Wachi Yuichi , Iwashita Mitsutoshi 
Published: July 2010

AbstractInsulin‐like growth factor (IGF)‐binding protein‐1 (IGFBP‐1), the main secretory protein of decidua that binds to IGFs and has been shown to inhibit or stimulate IGFs' bioactivities. Polymerization, one of the posttranslational modifications of IGFBP‐1, has been shown to lead to loss of inhibiting effect of IGFBP‐1 on IGF‐I actions. The current studies were undertaken to elucidate the effects of steroid hormones on IGFBP‐1 polymerization in trophoblast cell cultures. Placental tissues were obtained during legal, elective procedures of termination of pregnancy performed between 7 and 10 weeks of gestation, and primary trophoblast cells were separated. IGFBP‐1 polymerization was analyzed by SDS–PAGE and immunoblotting. IGFBP‐1 was polymerized when IGFBP‐1 was added to trophoblast cell cultures. Polymerization of IGFBP‐1 was inhibited by the addition of anti‐tissue transglutaminase antibody into the culture media. There was an increase in the intensity of polymerized IGFBP‐1 bands with the addition of medroxyprogesterone acetate (MPA), while no such difference was observed upon treatment with estradiol. MPA also increased the expression of tissue transglutaminase on trophoblast cell membranes. IGF‐I stimulated trophoblast cell migration, while IGFBP‐1 inhibited this IGF‐I‐induced trophoblast response. Addition of MPA attenuated the inhibitory effects of IGFBP‐1 on IGF‐I‐induced trophoblast cell migration. IGFBP‐1 was polymerized by tissue transglutaminase on the cell surface of trophoblasts, and MPA increased tissue transglutaminase expression on the cell surface and facilitated IGFBP‐1 polymerization. These results suggest that progesterone might facilitate polymerization of decidua‐secreted IGFBP‐1 and increase IGF‐I actions at feto‐maternal interface, thereby stimulating trophoblast invasion of maternal uterus. J. Cell. Physiol. 226: 434–439, 2011. © 2010 Wiley‐Liss, Inc.
Surface ultrastructure of larval mouthpart sensilla of the muga silkmoth, Antheraea assamensis, an endemic species of North‐East India
Authors: Dey Sudip , Singh Surendra , Chakraborty Rahul 
Published: July 2010

AbstractScanning electron microscopy of different larval stages of the muga silk moth Antheraea assamensis revealed the presence of sensilla chaetica, sensilla trichoidea, sensilla styloconica, gustatory sensilla, sensory pegs, placoid sensilla, etc., on their mouth parts. Some variations were observed in the number and sub‐types of sensilla in certain larval stages indicating some differences in sensitivity of the worms in different instars to the food plant and microhabitat. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.
Surface treatments and coatings to maintain fresh‐cut mango quality in storage
Authors: Plotto Anne , Narciso Jan A. , Rattanapanone Nithiya , Baldwin Elizabeth A 
Published: July 2010

AbstractBACKGROUND: Edible coatings may extend fresh‐cut fruit storage by preventing moisture loss and decreasing gas exchange. This study evaluated the effect of an antibrowning dip (calcium ascorbate, citric acid and N‐acetyl‐L‐cysteine), followed or not with carboxymethylcellulose (CMC) or carrageenan coatings on quality of fresh‐cut mangoes stored at 5 °C for up to 20 days. A fourth treatment, only used in one of four experiments, consisted of chitosan. Treatments were applied on ‘Tommy Atkins’, ‘Kent’ and ‘Keitt’ mangoes harvested from Homestead (FL), and on imported store‐bought mangoes.RESULTS: The antibrowning dips maintained the best visual quality during storage for all cultivars, as indicated by higher b*, hue and L*. The CMC coating maintained similar visual quality, but carrageenan or chitosan decreased L* and b*. The antibrowning dip containing calcium ascorbate reduced firmness loss on cut pieces of ‘Keitt’, ‘Kent’ and store‐bought mangoes. The antibrowning treatment maintained higher titratable acidity for ‘Kent’ and ‘Keitt’, resulting in lower sensory sweetness.CONCLUSION: This study with repeated experiments showed that calcium ascorbate with citric acid and N‐acetyl‐L‐cysteine maintained cut mango slices attractiveness in storage by keeping light color in both varieties. The addition of a polysaccharide coating did not consistently improve quality. Published in 2010 by John Wiley & Sons, Ltd.
L1, β‐catenin, and E‐cadherin expression in patients with colorectal cancer: Correlation with clinicopathologic features and its prognostic significance
Authors: Fang Qing‐Xia , Lü Liang‐Zhong , Yang Bo , Zhao Zhong‐Sheng , Wu Yue , Zheng Xiao‐Chun 
Published: July 2010

AbstractObjectiveCurrently, there are no clinically used/routine biomarkers that accurately predict whether colorectal cancer (CRC) patients will or will not respond to adjuvant chemotherapy. The aim of this study was to investigate L1, β‐catenin, and E‐cadherin expression in patients with CRC and their relationship to tumor progression, and to identify patients who will respond to chemotherapy.MethodsA total of 142 patients who underwent surgical treatment for CRC were chosen retrospectively. The samples from these patients were analyzed by immunohistochemistry. SPSS‐14 program package was used for statistical calculation.ResultsExpression of L1, β‐catenin, and E‐cadherin were found to be strongly associated with invasion and metastasis of CRC. Cox multivariate analysis results indicated that L1 expression and stage of Dukes could be considered as the independent prognostic factors for survival. Furthermore, our study found that the 5‐year survival rate was the significantly associated with the expression of L1, β‐catenin (normal and ectopic expression), and E‐cadherin for Dukes' stage B (P < 0.01) patients. However, no such result was found for Dukes' stage A (P > 0.05) and C (P > 0.05) patients.ConclusionOur study provided reference for identifying patients who need adjuvant chemotherapy. L1, β‐catenin, and E‐cadherin could be considered as biomarkers to predict whether CRC patients will or will not respond to adjuvant chemotherapy. J. Surg. Oncol. 2010;102:433–442. © 2010 Wiley‐Liss, Inc.
Human papillomavirus and oropharynx cancer: Biology, detection and clinical implications
Authors: Allen Clint T. , Lewis James S. , El‐Mofty Samir K. , Haughey Bruce H. , Nussenbaum Brian 
Published: July 2010

AbstractObjectives:To review evidence for the role of human papillomavirus (HPV) in the etiology of oropharyngeal cancers, methods of viral detection, and the resulting clinical implications.Study Design:Contemporary review.Methods:Published journal articles identified through PubMed and conference proceedings were reviewed.Results:HPV‐associated squamous cell carcinomas represent a distinct disease entity from carcinogen‐associated squamous cell carcinomas. HPV oncoproteins lead to mucosal cell transformation through well‐defined mechanisms. Different methods of detecting HPV exist with variable levels of sensitivity and specificity for biologically active virus. Although virus is detected in a number of head and neck subsites, studies demonstrate improved outcomes in HPV‐associated carcinoma of the oropharynx only. The cell cycle regulatory protein p16 is upregulated by biologically active HPV and serves as a biomarker of improved response to therapy.Conclusions:HPV‐associated squamous cell carcinoma of the oropharynx is a biologically distinct entity from carcinogen‐associated carcinoma. Understanding the molecular mechanisms behind the improved outcomes in patients with HPV‐associated oropharyngeal carcinoma may lead to novel therapeutics for patients with carcinogen‐associated carcinomas. Laryngoscope, 2010
Low‐protein and protein‐redistribution diets for Parkinson's disease patients with motor fluctuations: A systematic review
Authors: Cereda Emanuele , Barichella Michela , Pedrolli Carlo , Pezzoli Gianni 
Published: July 2010

AbstractThe American Academy of Neurology suggests advising the redistribution of daily protein meal content to every Parkinson's disease (PD) patient with motor fluctuations during levodopa treatment. However, no comprehensive evaluation of this complementary therapy has been performed. A systematic review of intervention studies investigating the neurologic outcome of low‐protein (<0.8 g/kg of ideal weight/day) and protein‐redistribution diets in patients with PD experiencing motor fluctuations during levodopa treatment. All studies (uncontrolled or randomized) investigating a low‐protein and/or a protein‐redistribution diet (LPD and PRD) and involving patients with PD with motor fluctuations were included, provided that sufficient information on dietary protein content and neurologic outcome measures was available. We identified 16 eligible studies, but they were markedly heterogeneous. There was not enough evidence to support the use of LPD. Response to PRD seemed very good. Acceptability appeared high upon introduction, but it seemed to progressively decrease over time. On average, PRD resulted in improved motor function, but also complications occurred. At the beginning, drop‐outs were due to levodopa side effects rather than unsatisfactory benefits. Long‐term adherence was more affected by changes in dietary habits than by diet‐related side effects. Efficacy and benefits appeared to be higher when the intervention was proposed to subjects in the early stages of PD. PRD can be safely advised to fluctuating patients with PD, but those in whom benefits override the possible inconveniences still need to be identified. The long‐term effects of PRD on nutritional status should be evaluated and true effectiveness in clinical practice should be reassessed, given the changes in levodopa formulations and the introduction of several adjuvants (levodopa degradation inhibitors and/or dopamine agonists). © 2010 Movement Disorder Society.
Anxious personality predicts an increased risk of Parkinson's disease
Authors: Bower James H. , Grossardt Brandon R. , Maraganore Demetrius M. , Ahlskog J. Eric , Colligan Robert C. , Geda Yonas E. , Therneau Terry M. , Rocca Walter A. 
Published: July 2010

AbstractWe studied the association of three personality traits related to neuroticism with the subsequent risk of Parkinson's disease (PD) using a historical cohort study. We included 7,216 subjects who resided within the 120‐mile radius centered in Rochester, MN, at the time they completed the Minnesota Multiphasic Personality Inventory (MMPI) for research at the Mayo Clinic from 1962 to 1965. We considered three MMPI personality scales (pessimistic, anxious, and depressive traits). A total of 6,822 subjects (94.5%) were followed over four decades either actively or passively. During follow‐up, 227 subjects developed parkinsonism (156 developed PD). An anxious personality was associated with an increased risk of PD [hazard ratio (HR), 1.63; 95% confidence interval (CI), 1.16–2.27]. A pessimistic personality trait was also associated with an increased risk of PD but only in men (HR = 1.92; 95% CI = 1.20–3.07). By contrast, a depressive trait was not associated with increased risk. Analyses combining scores from the three personality scales into a composite neuroticism score showed an association of neuroticism with PD (HR = 1.54; 95% CI = 1.10–2.16). The association with neuroticism remained significant even when the MMPI was administered early in life (ages 20–39 years). By contrast, none of the three personality traits was associated with the risk of non‐PD types of parkinsonism grouped together. Our long‐term historical cohort study suggests that an anxious personality trait may predict an increased risk of PD developing many years later. © 2010 Movement Disorder Society.
Novel pathogenic LRRK2 p.Asn1437His substitution in familial Parkinson's disease
Authors: Aasly Jan O. , Vilariño‐Güell Carles , Dachsel Justus C. , Webber Philip J. , West Andrew B. , Haugarvoll Kristoffer , Johansen Krisztina K. , Toft Mathias , Nutt John G. , Payami Haydeh , Kachergus Jennifer M. , Lincoln Sarah J. , Felic Amela , Wider Christian , Soto‐Ortolaza Alexandra I. , Cobb Stephanie A. , White Linda R. , Ross Owen A. , Farrer Matthew J. 
Published: July 2010

AbstractGenealogical investigation of a large Norwegian family (F04) with autosomal dominant parkinsonism has identified 18 affected family members over four generations. Genetic studies have revealed a novel pathogenic LRRK2 mutation c.4309 A>C (p.Asn1437His) that co‐segregates with disease manifestation (LOD = 3.15, θ = 0). Affected carriers have an early age at onset (48 ± 7.7 SD years) and are clinically asymmetric and levodopa responsive. The variant was absent in 623 Norwegian control subjects. Further screening of patients from the same population identified one additional affected carrier (1 of 692) with familial parkinsonism who shares the same haplotype. The mutation is located within the Roc domain of the protein and enhances GTP‐binding and kinase activity, further implicating these activities as the mechanisms that underlie LRRK2‐linked parkinsonism. © 2010 Movement Disorder Society
Findings on how adolescents cope with cancer—a matter of methodology?
Authors: Engvall G. , Mattsson E. , von Essen L. , Hedström M. 
Published: July 2010

AbstractObjectives: The various conclusions drawn from previous studies on how adolescents cope with cancer might partly be explained by methodological issues. The aim was to explore how adolescents recently diagnosed with cancer report that they cope with disease‐ and treatment‐related distress in response to closed‐ and open‐ended questions, respectively.Methods: Adolescents diagnosed with cancer 4–8 weeks ago (N=56) answered closed‐ and open‐ended questions over the telephone about which coping strategies they use to cope with physical concerns, personal changes, feelings of alienation, and worries.Results: In response to closed‐ended questions, most adolescents reported using emotion‐focused coping (Accepting and Minimising) while, in response to open‐ended questions, meaning‐based (i.e. Positive thinking) and problem‐focused (i.e. Problem solving) coping were most often mentioned. A majority reported using Minimising and Seeking support in response to closed‐ended questions, but very few adolescents mentioned using these strategies in response to open‐ended questions.Conclusions: Adolescents' reports of how they cope with disease‐ and treatment‐related distress vary depending on antecedent closed‐ and open‐ended questions. Responses to closed‐ended questions appear to be more indifferent to aspects of distress than responses to open‐ended questions. Strategies representing meaning‐based coping should be included in future studies investigating how adolescents recently diagnosed with cancer cope with disease‐ and treatment‐related distress. Copyright © 2010 John Wiley & Sons, Ltd.
Patterns, correlates, and prognostic significance of quality of life following breast cancer
Authors: DiSipio Tracey , Hayes Sandi , Battistutta Diana , Newman Beth , Janda Monika 
Published: July 2010

AbstractObjective: To describe quality of life (QOL) over a 12‐month period among women with breast cancer, consider the association between QOL and overall survival (OS), and explore characteristics associated with QOL declines.Methods: A population‐based sample of Australian women (n=287) with invasive, unilateral breast cancer (Stage I+) was observed prospectively for a median of 6.6 years. QOL was assessed at 6, 12, and 18 months post‐diagnosis, using the Functional Assessment of Cancer Therapy, Breast (FACT‐B+4) questionnaire. Raw scores for the FACT‐B+4 and subscales were computed and individuals were categorized according to whether QOL declined, remained stable or improved between 6 and 18 months. Kaplan–Meier and Cox proportional hazards survival methods were used to estimate OS and its associations with QOL. Logistic regression models identified factors associated with QOL decline.Results: Within FACT‐B+4 subscales, between 10% and 23% of women showed declines in QOL. Following adjustment for established prognostic factors, emotional well‐being and FACT‐B+4 scores at 6 months post‐diagnosis were associated with OS (p<0.05). Declines in physical (p<0.01) or functional (p=0.02) well‐being between 6 and 18 months post‐diagnosis were also associated significantly with OS. Receiving multiple forms of adjuvant treatment, a perception of not handling stress well and reporting one or more other major life events at 6 months post‐diagnosis were factors associated with declines in QOL in multivariable analyses.Conclusions: Interventions targeted at preventing QOL declines may ultimately improve quantity as well as quality of life following breast cancer. Copyright © 2010 John Wiley & Sons, Ltd.
Gender differences in the Scandinavian cystic fibrosis population
Authors: Olesen Hanne Vebert , Pressler Tacjana , Hjelte Lena , Mared Lena , Lindblad Anders , Knudsen Per Kristian , Lærum Birger N. , Johannesson Marie ,   on behalf of the Scandinavian Cystic Fibrosis Study Consortium
Published: July 2010

AbstractAimsTo explore whether gender differences in the Scandinavian Cystic Fibrosis (CF) patients exist in the areas of key clinical parameters, complications, and medication.MethodsCross‐sectional data on 890 (416 female) pancreatic insufficient CF patients were evaluated regarding chronic infection, body mass index, lung function, medication, and diabetes, as well as data of Pseudomonas infection status, antibiotic treatment and hospitalization from 1‐year follow‐up.ResultsWe found no differences in lung function, body mass index, or frequency of diabetes. The adult group consisted of more males than females (208:168). We found no significant difference in prevalence of chronic Pseudomonas aeruginosa infection, but during the follow‐up the incidence of new chronic infection was higher in adult females (10/33 vs. 4/56). Females had higher prevalence of Burkholderia infection (21/416 vs. 11/474). Adult females had more days on intravenous antibiotics (median 39 vs. 26 days/year), and days in hospital (median 2 vs. 0 days/year). More adult females received inhaled and oral steroids. In the pediatric cohort, females were treated more often with macrolides as an anti‐inflammatory agent.ConclusionWe found no gender difference in key clinical parameters in our CF population. However, our study showed a higher risk of Pseudomonas and Burkholderia infection among the female patients. Additionally, we found that female patients require more intensified treatment regarding antibiotics, macrolides, steroids and days of hospitalization, indicating a true female disadvantage even with modern aggressive treatment. The finding of more males than females in the adult population suggesting a male advantage, warrants a mortality study. Pediatr Pulmonol. 2010; 45:959–965. © 2010 Wiley‐Liss, Inc.
Comparison of the activity of non‐steroidal ecdysone agonists between dipteran and lepidopteran insects, using cell‐based EcR reporter assays
Authors: Soin Thomas , Swevers Luc , Kotzia Georgia , Iatrou Kostas , Janssen Colin R , Rougé Pierre , Harada Toshiyuki , Nakagawa Yoshiaki , Smagghe Guy 
Published: July 2010

AbstractBACKGROUND: Diacylhydrazine (DAH) analogues have been developed successfully as a new group of insect growth regulators, called ecdysone agonists or moulting accelerating compounds. These DAHs have been shown to manifest their toxicity via interaction with the ecdysone receptor (EcR) in susceptible insects, as does the natural insect moulting hormone 20‐hydroxyecdysone (20E). A notable feature is their high activity and specificity, particularly against lepidopteran insects, raising the question as to whether non‐lepidopteran‐specific analogues can be isolated. However, for the discovery of ecdysone agonists that target other important insect groups such as Diptera, efficient screening systems that are based on the activation of the EcR are needed.RESULTS: In this study, a dipteran‐specific reporter‐based screening system with transfected S2 cells of Drosophila melanogaster Meig. was developed in order to discover and evaluate compounds that have ecdysone agonistic or antagonistic activity. A library of non‐steroidal ecdysone agonists containing different mother structures with DAH and other related analogues such as acylaminoketone (AAK) and tetrahydroquinoline (THQ) was tested. None of the compounds tested was as active as 20E. This is in contrast to the very high activity of several DAH and AAK congeners in lepidopteran cells (Bombyx mori L.‐derived Bm5 cells). The latter agrees with a successful docking of a DAH, tebufenozide, in the binding pocket of the lepidopteran EcR (B. mori), while this was not the case with the dipteran EcR (D. melanogaster). Of note was the identification of two THQ compounds with activity in S2 but not in Bm5 cells. Although marked differences in activity exist with respect to the activation of EcR between dipterans and lepidopterans, there exists a positive correlation (R = 0.724) between the pLC50 values in S2 and Bm5 cells. In addition, it was found through protein modelling that a second lobe was present in the ligand‐binding pocket of lepidopteran BmEcR but was lacking in the dipteran DmEcR protein, suggesting that this difference in structure of the binding pocket is a major factor for preferential activation of the lepidopteran over the dipteran receptors by DAH ligands.CONCLUSIONS: The present study confirmed the marked specificity of DAH and AAK analogues towards EcRs from lepidopteran insects. THQ compounds did not show this specificity, indicating that dipteran‐specific ecdysone‐agonist‐based insecticides based on the THQ mother structure can be developed. The differences in activity of ecdysone agonists in dipteran and lepidopteran ecdysone‐reporter‐based screening systems are discussed. Copyright © 2010 Society of Chemical Industry
GaN and ZnO nanostructures
Authors: Fündling Sönke , Sökmen Ünsal , Behrends Arne , Al‐Suleiman Mohamed Aid Mansur , Merzsch Stephan , Li Shunfeng , Bakin Andrey , Wehmann Hergo‐Heinrich , Waag Andreas , Lähnemann Jonas , Jahn Uwe , Trampert Achim , Riechert Henning 
Published: July 2010

AbstractGaN and ZnO are both wide band gap semiconductors with interesting properties concerning optoelectronic and sensor device applications. Due to the lack or the high costs of native substrates, alternatives like sapphire, silicon, or silicon carbide are taken, but the resulting lattice and thermal mismatches lead to increased defect densities which reduce the material quality. In contrast, nanostructures with high aspect ratio have lower defect densities as compared to layers. In this work, we give an overview on our results achieved on both ZnO as well as GaN based nanorods. ZnO nanostructures were grown by a wet chemical approach as well as by VPT on different substrates – even on flexible polymers. To compare the growth results we analyzed the structures by XRD and PL and show possible device applications. The GaN nano‐ and microstructures were grown by metal organic vapor phase epitaxy either in a self‐organized process or by selective area growth for a better control of shape and material composition. Finally we take a look onto possible device applications, presenting our attempts, e.g., to build LEDs based on GaN nanostructures.
Dilute magnetic semiconductors from electrodeposited ZnO nanowires
Authors: Nadarajah A. , Könenkamp R. 
Published: July 2010

AbstractWe present experimental results on the magnetic properties of doped ZnO nanowires grown at 80 °C in electrodeposition. We show that impurities such as Al, Mn, Co, and Cu can be incorporated in the nanowires by adding the corresponding metal salts to the electrolyte solution. At concentration levels of a few atomic percent we find the impurity concentration in the solid to be approximately proportional to the precursor concentration in solution. ZnO nanowrires doped with Cu, Co, and Mn show superparamagnetic response at room temperature, while undoped and Al‐doped wires show no discernible magnetic response. The results indicate that with Cu, Mn, and Co doping dilute magnetic semiconductors can be prepared.