bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upaya Dinas Koperasi, Kredit Usaha Kecil Dan Menengah (KUKM) dalam Meningkatkan Kinerja Pengurus Koperasi Pegawai Negeri (KPN) Tri Daya di Kabupaten Timor Tengah Utara
Authors: SENGKOEN DELFIANA 
Published: April 2024

Penelitian ini bertujuan untuk mengetahui upaya dinas koperasi, dan upaya peningkatan kinerja pengurus Koperasi Pengawai Negeri (KPN) Tri Daya di Kabupaten TTU. Jenis penelitian yang digunakan dalam penelitian ini adalah metode kualitatif dengan menggunakan data primer dan data sekunder berupa hasil wawancara dengan informan penelitian. Hasil dari penelitian ini adalah upaya dinas koperasi dalam meningkatkan kinerja pengurus Koperasi Pegawai Negeri (KPN) Tri Daya Kabupaten TTU. Dalam meningkatkan kinerja pengurus melalui pendidikan, pelatihan SDM terhadap koperasi telah dilakukan namun kurangnya keterlibatan pengurus koperasi dan semua pengurus koperasi masih banyak kekurangan ilmu sehingga belum mampu mengelola administrasi keuangan dengan baik. Pemerintah melakukan pengawasan dan pembinaan terhadap koperasi agar segala kegiatan dilakukan dapat berjalan lancar, bersih, aman sesuai dengan peraturan yang sudah ditetapkan oleh pihak pemerintah. Penyertaan modal dari Dinas Koperasi belum tersedia hanya ada investasi modal yang tidak permanen karna pemerintah memberikan sumber dana hanya melalui APBD dan APBN.
DEMOKRASI LOKAL ( TOKOM TABUA HET TAFEKAN LASI ) DALAM PROSES PEMILIHAN KEPALA DESA DI DESA LOEL KECAMATAN INSANA FAFINESU KABUPATEN TIMOR TENGAH UTARA
Authors: Silva Antonius Da , Festianto Dian , Naif Yohanes I 
Published: April 2024

“Demokrasi Lokal (Tabua Het Tafekan Lasi) Dalam Proses Pemilihan Kepala Desa Di Desa Loel Kecamatan Insana Fafinesu Kabupaten Timor Tengah Utara “(Studi Kasus Pada Desa Loel Kecamatan Insana Fafinesu Kabupaten Timor Tengah Utara). Tujuan dari penelitian ini untuk a). mengetahui proses dan dinamika terjadinya demokrasi lokal didesa Loel Kecamatan Insana Fafinesu. b). Untuk memberikan suatu gambaran (Deskriptif) tentang Demokrasi Lokal dalam proses pemilihan kepala desa di Desa Loel Kecamata Insana Fafinesu. Model yang digunakan oleh penelitian ini yakni metode kualitatif jenis penelitian deskriptif kualitatif kerap yang digunakan untuk menganalisis kejadian, fenomena, atau keadaan secara sosial dengan teknik pengumpulan data melalui observasi, wawancara serta dokumentasi. Peneliti mengumpulkan sebanyak 14 informen agar mendapatkan berbagai informasi terkait permasalahan yang ada di Desa Loel.
(Food) Hoarding
Authors: Huỳnh Lenora 
Published: April 2024

Food hoarding in human behaviour, on its own, has not been frequently documented or extensively researched within archival studies. Various reasons may contribute to a lack of available information on food hoarding. A primary example is that hoarders often experience feelings of guilt, shame, and dispossession of their hoardings. Accessible materials in food hoarding research, however, do include historically archived propaganda posters from world wars and news articles that described the scarcity mindset of citizens during times of crisis, including wars, genocides, and pandemics. Clinical and sociological research has often provided a general analysis of hoarding that informs researchers of the issues that hoarders deal with in private. However, I propose that research on general hoarding should extend to observing behaviours around food and its interconnection to memory and trauma. Furthermore, I believe that the literature about theories of hoarding as an act of archiving—although there are very few—adds to the perceptions of displaced communities’ experiences with food insecurity and attachment. These new methods of examining the archive explain how the displaced have preserved their pasts, challenged their present through mobility (or lack thereof), and planned for their futures through knowledge beyond a traditional archive.
Planting The Seed
Authors: Grigoli Rebecca 
Published: April 2024

Cultural preservation is an evolving phenomenon that has historically been undertaken through the written or oral passage of knowledge from one generation to the next. Some archives emerge so individuals can understand their social, cultural, and personal histories, while others emerge to preserve non-human-related specimens to prepare for worst-case scenarios. This paper discusses the notion of the “doomsday” archive and connects it to concepts of archival practice by exploring the history and efficacy of the Svalbard Global Seed Vault and the various ways humans have used archival principles to mitigate harm in the face of global catastrophes.
Validation of an enzyme-linked immunosorbent assay test-system for bevacizumab concentration determing in biological fluids
Authors: Pisarev V. V. , Ivanov A. V. 
Published: April 2024

Introduction. One of the reasons for the development of many diseases primarily malignant is the increased expression of Vascular Endothelial Growth Factor (VEGF). Bevacizumab is a drug that neutralizes the biological activity of VEGF. The molecular structure of bevacizumab is a recombinant humanized antibody. Its use reduces vascularization in the foci of increased VEGF expression which slows down tumor growth and also helps restore vision in a number of ophthalmic diseases. To determine the concentration of bevacizumab in human biological fluids a test system based on Enzyme-linked Immunosorbent Assay (ELISA) is presented.Aim. Aim of this study is the validation of this test system.Materials and methods. Blank sera of volunteers, bevacizumab solution, enzyme-linked immunosorbent assay, solid-phase sandwich ELISA kit, microplate photometer.Results and discussion. The following characteristics of the test system were determined: the lower limit of quantification is 2.0 mcg/ml, the upper calibration range is up to 200 mcg/ml, the accuracy and precision within one series and between series does not exceed 20 %, and the total error of the method – 30 %, short-term stability for samples at room temperature – 6 hours, long-term stability – 14 days at –20 °C, the ability to freeze/thaw of samples is up to three times, the ability to determine samples with a concentration above the upper calibrator after diluting in 2 times.Conclusion. The results obtained fully comply with international acceptance criteria and allow the use of the ELISA test system manufactured by LLC "Probiotek" for use in the field of clinical laboratory diagnostics.
Hepatoprotective and nephroprotective property of Zingiber officinale leaves in acetaminophen induced liver and kidney damage in Wistar rats
Authors: Ibukun O , Ibukun O 
Published: April 2024

It is necessary to search for safe compounds for management of kidney and liver diseases, due to sideeffects of currently available drugs. This research studied the hepatoprotective and nephroprotective effects of Zingiber officiale leaves in Wistar rats using acetaminophen as toxicant. Thirty rats were classified to five categories comprising of six animals each. The first group was the normal control and was given only vehicle (distilled water). Groups 2 served as acetaminophen control and was givenacetaminophen (750mg/kg). Groups 3, 4, 5 received acetaminophen (750mg/kg). Groups 3, 4, 5 also received silymarin (reference drug; 50mg/kg), Zingiber officiale (200mg/kg), Zingiber officiale (400mg/kg) respectively. All administration was done by oral means for 21 days. Experimental rats were thereafter euthanized by cervical dislocation. Blood and other tissues were harvested. Results showed that rats in normal control, silymarin and Zingiber officinale groups had significantly (p < 0.05)lower serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, urea and creatinine levels in comparison with rats in acetaminophen control. Rats in acetaminophen control had lower concentration of protein in serum, in comparison with normal control, silymarin or Zingiber officiale treated groups. Furthermore, reduced glutathioneconcentration was lowered and malondialdehyde concentration was raised in tissues (liver and kidney) in acetaminophen control rats in comparison with other groups. There was no significant difference in the ALP, AST, total protein, albumin, bilirubin, urea, creatinine, GSH and MDA levels of treatment groups (3, 4 and 5). These findings imply that methanol leaf extract of Zingiber officiale possess hepatoprotective, nephroprotective and antioxidant effects in rats. The study is therefore relevantin the discovery of new compounds for ameliorating the burden of liver and kidney diseases.
Evaluation of fungal permeated lignocellulosic filter for remediation of water pollutants
Authors: Titilawo M. A. , Adeyemi F. M. , Ajani T. F. , Ogunlana E. O. , Titilawo Y. 
Published: April 2024

The use of biological techniques to remove pollutants from the environment has been long advocated. In this study, we assessed the efficacy of a fungal permeated lignocellulosic filter (mycofilter) in the removal of water pollutants using surface water as the test sample. A wild macrofungus was collected from a field within Osun State University, Oke-Baale, Osogbo, and cultured on potato dextrose agar. Identification was achieved using morphological and PCR techniques. Mycofilters (FCCs) were developed from pure macrofungus mycelium and diced pasteurized corncob (CC). Collected surface water (RW) was passedthrough three mycofilters, each with flow rates of 0.2, 0.5, and 1.0 ml/min. Diced pasteurized corncob (CC) was used as the negative control. Physicochemical parameters including pH, temperature, turbidity, cadmium, copper, iron, zinc, and lead, and microbial parameters i.e. total bacterial count (TBC) and Escherichia coli counts (ECC) of RW, CC, and FCC treated waters were determined. Phenotypically, the fungus was identified as Macrolepiota procera and yielded 650 bp by PCR product assessment on gel electrophoresis. Remarkably, FCC reduced the levels of turbidity, chemical, and microbial contaminants significantly at p = 0.001, < 0.05, and ≤ 0.002 respectively compared to CC. The flow rate of 0.2 ml/min was the most efficient in reducing contaminants in the RW. This study reveals the potential of the Macrolepiota procera permeated filter in reducing water contaminants.
Characterization of amorphous silicon nitride prepared from sand and coffee husk wastes by carbothermal reduction-nitridation
Authors: Ndung'u S. N. , Nthiga E. W. , Wanjau R. N. 
Published: April 2024

The continued coffee demand in Kenya has amplified the generation of its husk wastes causing disposal problems. This has led to serious pollution to the environment. Therefore, developing a greener and cost-effective ways to handle these wastes is necessary. The current study entailed the use of extracted silica and coffee husk biochar as novel precursor materials for the synthesis of silicon nitride .....
Preperitoneal pelvic balloon tamponade—an effective intervention to control pelvic injury hemorrhage in a swine model
Authors: Jin Xiaogao , Chu Qinjun , Bing Hailong , Li Fang , Bai Jingyue , Lou Junge , Sun Liwei , Zhang Chenxi , Lin Lin , Li Liumei , Wang Haibo , Zhou Zhanfeng , Zhang Junfeng , Lian Hongkai 
Published: April 2024

Objective: This study aimed to estimate the effects of the volume of preperitoneal balloon (PPB) on arterial and venous hemorrhage in a swine pelvic fracture model.Methods: Twenty-four swine were randomized into 0-mL, 500-mL, 800-mL, and 1000-mL intra-hematoma PPB groups. They were subjected to open-book pelvic fracture and reproducible injuries in the external iliac artery and vein. The pelvic binder and IH-PPBs with different volumes of fluid were applied to control the active hemorrhage after arterial and venous injuries. The survival time and rate during 60-min observation and digital subtraction angiography (DSA) images were the primary endpoints in this study. Secondary endpoints included survival rate within 70 min, peritoneal pressure, hemodynamics, blood loss, infusion fluid, blood pH, and lactate concentration.Results: Our results indicated that the 800-mL and 1000-mL groups had a higher survival rate (0%, 50%, 100% and 100% for 0, 500, 800, and 1000-mL groups respectively; p &lt; 0.0001) and longer survival time (13.83 ± 2.64, 24.50 ± 6.29, 55.00 ± 6.33, and 60.00 ± 0.00 min for 0, 500, 800, and 1,000 groups respectively; p &lt; 0.0005) than the 0-mL or 500-mL groups during the 60 min observation. Contrastingly, survival rate and time were comparable between 800-mL and 1000-mL groups during the 60-min observation. The IH-PPB volume was associated with an increase in the pressure of the balloon and the preperitoneal pressure but had no effect on the bladder pressure. Lastly, the 1000-mL group had a higher mean arterial pressure and systemic vascular resistance than the 800-mL group.Conclusion: IH-PPB volume-dependently controls vascular bleeding after pelvic fracture in the swine model. IH-PPB with a volume of 800 mL and 1000 mL efficiently managed pelvic fracture-associated arterial and venous hemorrhage and enhanced survival time and rate in the swine model without evidences of visceral injury.
Case Report: Transcatheter aortic valve implantation using balloon-expandable bioprosthesis in patients with severe pure aortic regurgitation on noncalcified native valves: a series of cases
Authors: Santos Luciano de Moura , Luz Larissa Santos , Bastos Vinicius Lelis , Barcelos Tulio Assunção , Abreu Frederico André Alves , Beck Leonardo Cogo , Darnasser Mohammed Jamal Aldin Hilal , Cruz Francisco de Assis , Matos Luis Carlos Vieira , Carvalho Wenderval Borges 
Published: April 2024

BackgroundFor individuals with pure aortic regurgitation (AR), transcatheter aortic valve implantation (TAVI) is cautiously recommended only for those with a high or prohibitive surgical risk. We aimed to describe the results of a case series of transcatheter implantation of a balloon-expandable aortic valve bioprosthesis (BEV) for the treatment of noncalcified native valve AR.MethodsFrom February 2022–November 2022, we performed TAVI in patients with severe pure AR. Cases were indicated on the basis of symptoms, high/prohibitive surgical risk, or patient refusal of conventional treatment.ResultsFive patients underwent successful TAVI. The mean age was 81.9 ± 6.6 years, 3 (60%) female and 5 (100%) in NYHA class III or IV. The baseline echocardiogram showed an ejection fraction of 49.0 ± 10.6% and left ventricular end-systolic diameter 28.5 ± 4.7 mm/m². The average area of the aortic annulus was 529.1 ± 47.0mm² and the area oversizing index was 17.6 ± 1.2%. In the 30-day follow-up, there were no cases of prosthesis embolization, annulus rupture, stroke, acute myocardial infarction, acute renal failure, hemorrhagic complication or death. One patient required a permanent pacemaker and another had a minor vascular complication. The clinical follow-up were 19.8 months (16.7–21.8). During this period, all patients remained alive and in NYHA class I or II. One of the patients developed a moderate paravalvular leak.ConclusionTAVI with a BEV proved to be safe and effective in this small case series of patients with noncalcified native valve AR in a follow-up longer than 1 year.
The landscape of genetic variations in non-syndromic primary ovarian insufficiency in the MENA region: a systematic review
Authors: Allouch Asma , Al-Barazenji Tara , Al-Shafai Mashael , Abdallah Atiyeh M. 
Published: April 2024

IntroductionPremature ovarian insufficiency (POI) is a primary cause of infertility with variable clinical manifestations. POI is a multifactorial disease with both environmental and known genetic etiologies, but data on the genetic variations associated with POI in the Middle East and North Africa (MENA) region are scarce. The aim of this study was to systematically review all known genetic causes of POI in the MENA region.MethodsThe PubMed, Science Direct, ProQuest, and Embase databases were searched from inception to December 2022 for all reports of genetic variants associated with POI in the MENA region. Clinical and genetic data were collected from eligible articles, and ClinVar and PubMed (dbSNP) were searched for variants.ResultsOf 1,803 studies, 25 met the inclusion criteria. Fifteen studies were case-control studies and ten were case reports representing 1,080 non-syndromic POI patients in total. Seventy-nine variants in 25 genes associated with POI were reported in ten MENA countries. Of the 79 variants, 46 were rare and 33 were common variants. Of the 46 rare variants, 19 were pathogenic or likely pathogenic according to ACMG classification guidelines and ClinVar. No clear phenotype-genotype association was observed. Male family members carrying pathogenic variants also had infertility problems.DiscussionTo our best knowledge, this is the first systematic review of the genetic variants associated with POI in the MENA region. Further functional studies are needed to assess the disease-causing molecular mechanisms of these variants. Knowledge of the genetic basis of POI in the Middle East could facilitate early detection of the condition and thus early implementation of therapeutic interventions, paving the way for precision medicine options in specific populations.
Haemoglobin, albumin, lymphocyte, and platelet score as an independent predictor for renal prognosis in IgA nephropathy
Authors: Yuan Yuan , Liang Xiaoli , He Minhui , Wu Yufan , Jiang Xue 
Published: April 2024

ObjectiveThe haemoglobin, albumin, lymphocyte, and platelet (HALP) score, a convenient and composite laboratory biomarker, can reflect inflammation and systemic nutritional status. This study was performed to investigate the effect of the HALP score on the prognosis of patients with IgA nephropathy (IgAN).MethodsThis is a retrospective single centre study that enrolled 895 biopsy-confirmed IgAN patients from June 2019 to June 2022 who were followed for more than 1 year. Kaplan–Meier curves and Cox regression analyses were performed to determine the relationship between HALP and adverse outcomes. The restricted cubic splines was used to identify the possible associations. The optimal cut-off value of HALP for renal poor outcome was identified by the area under the receiver operating characteristic curve (AUC).ResultsA total of 895 patients finally participated in the study and were divided into three groups (tertial 1-3) according to the baseline HALP score. More severe clinicopathologic features were observed in the lower HALP group, and Kaplan−Meier analysis showed patients in tertial 1 had a higher risk of kidney failure than the other groups (log-rank=11.02, P= 0.004). Multivariate Cox regression revealed that HALP score was an independent risk factor for renal prognosis in IgAN (adjusted HR: 0.967, 95% CI: 0.945-0.990, P = 0.006). The results of subgroup analysis suggested that HALP was more important in patients under the age of 50, BMI ≤ 23.9 and eGFR ≤ 90 mL/min/1.73 m2. The best cut-off HALP for renal survival was 38.83, sensitivity 72.1%, and specificity 55.9% (AUC: 0.662). Patients were further grouped according to HALP cut-off values and propensity matched. Multivariate Cox regression analysis revealed that HALP remained an independent predictor of IgAN in the matched cohort (HR 0.222, CI: 0.084-0.588, P=0.002).ConclusionHALP is a novel and potent composite parameter to predict kidney outcome in patients with IgAN.
Multifactorial determinants of NK cell repertoire organization: insights into age, sex, KIR genotype, HLA typing, and CMV influence
Authors: Ferron Enora , David Gaëlle , Willem Catherine , Legrand Nolwenn , Salameh Perla , Anquetil Laetitia , Walencik Alexandre , Gendzekhadze Ketevan , Gagne Katia , Retière Christelle 
Published: April 2024

IntroductionPolymorphisms in the KIR and HLA genes contribute to the diversity of the NK cell repertoire. Extrinsic factors also play a role in modifying this repertoire. The best example is cytomegalovirus, which promotes the expansion of memory-like NK cells. However, the mechanisms governing this phenotypic structure are poorly understood. Furthermore, the influence of age and sex has been understudied.MethodsIn this study, we examined these parameters in a cohort of 200 healthy volunteer blood donors, focusing on the major inhibitory KIR receptors and CD94/NKG2A, as well as the differentiation marker CD57 and the memory-like population marker NKG2C. Flow cytometry and two joint analyses, unsupervised and semi-supervised, helped define the impact of various intrinsic and extrinsic markers on the phenotypic structure of the NK cell repertoire. ResultsIn the KIR NK cell compartment, the KIR3DL1 gene is crucial, as unexpressed alleles lead to a repertoire dominated by KIR2D interacting only with HLA-C ligands, whereas an expressed KIR3DL1 gene allows for a greater diversity of NK cell subpopulations interacting with all HLA class I ligands. KIR2DL2 subsequently favors the KIR2D NK cell repertoire specific to C1/C2 ligands, whereas its absence promotes the expression of KIR2DL1 specific to the C2 ligand. The C2C2Bw4+ environment, marked by strong -21T motifs, favors the expansion of the NK cell population expressing only CD57, whereas the absence of HLA-A3/A11 ligands favors the population expressing only NKG2A, a population highly represented within the repertoire. The AA KIR genotype favors NK cell populations without KIR and NKG2A receptors, whereas the KIR B+ genotypes favor populations expressing KIR and NKG2A. Interestingly, we showed that women have a repertoire enriched in CD57- NK cell populations, while men have more CD57+ NK cell subpopulations. DiscussionOverall, our data demonstrate that the phenotypic structure of the NK cell repertoire follows well-defined genetic rules and that immunological history, sex, and age contribute to shaping this NK cell diversity. These elements can contribute to the better selection of hematopoietic stem cell donors and the definition of allogeneic NK cells for cell engineering in NK cell-based immunotherapy approaches.cters are displayed correctly.