bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marinomonas rhodophyticola sp. nov. and Marinomonas phaeophyticola sp. nov., isolated from marine algae
Authors: Butt Mahrukh , Choi Dae Gyu , Kim Jeong Min , Lee Jae Kyeong , Baek Ju Hye , Jeon Che Ok 
Published: May 2024

Two Gram-stain-negative, facultatively aerobic, and motile rod bacteria, designated as strains KJ51-3T and 15G1-11T, were isolated from marine algae collected in the Republic of Korea. Both strains exhibited catalase- and oxidase-positive activities. Optimum growth conditions for strain KJ51-3T were observed at 30 °C and pH 6.0–8.0, with 1.0–7.0 % (w/v) NaCl, whereas strain 15G1-11T exhibited optimal growth at 30 °C, pH 7.0, and 1.0–5.0 % NaCl. Major fatty acids detected in both strains included C16 : 0, C10 : 0 3-OH and summed features 3 (C16 : 1
ω7c and/or C16 : 1
ω6c) and 8 (C18 : 1
ω7c and/or C18 : 1
ω6c). As for polar lipids, strain KJ51-3T contained phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol, and two unidentified phospholipids, whereas strain 15G1-11T had PE, PG, and an unidentified aminolipid. Ubiquinone-8 was the predominant respiratory quinone in both strains, with minor detection of ubiquinone-9 in strain KJ51-3T. The genomic DNA G+C contents were 44.0 mol% for strain KJ51-3T and 40.5 mol% for strain 15G1-11T. Phylogenetic analyses based on both 16S rRNA gene and genome sequences placed strains KJ51-3T and 15G1-11T into distinct lineages within the genus Marinomonas, most closely related to Marinomonas arctica 328T (98.6 %) and Marinomonas algicola SM1966T (98.3 %), respectively. Strains KJ51-3T and 15G1-11T exhibited a 94.6 % 16S rRNA gene sequence similarity and a 70.7 % average nucleotide identity (ANI), with ANI values of 91.9 and 79.3 % between them and M. arctica 328T and M. algicola SM1966T, respectively, indicating that they represent novel species. In summary, based on their phenotypic, chemotaxonomic, and phylogenetic properties, strains KJ51-3T and 15G1-11T are proposed to represent novel species within the genus Marinomonas, for which the names Marinomonas rhodophyticola sp. nov. (KJ51-3T=KACC 22756T=JCM 35591T) and Marinomonas phaeophyticola sp. nov. (15G1-11T=KACC 22593T=JCM 35412T) are respectively proposed.
Attaching artificial Achilles and tibialis cranialis tendons to bone using suture anchors in a rabbit model: assessment of outcomes
Authors: Fidelis Obinna P. , Stubbs Caleb , Easton Katrina L. , Billings Caroline , Pedersen Alisha P. , Anderson David E. , Crouch Dustin L. 
Published: May 2024

ABSTRACTObjectiveThe purpose of this study was to investigate the factors associated with outcomes of attaching artificial tendons to bone using suture anchors for replacement of biological tendons in rabbits.Study DesignMetal suture anchors with braided composite sutures of varying sizes (USP #1, #2, or #5) were used to secure artificial tendons replacing both the Achilles and tibialis cranialis tendons in 12 New Zealand White rabbits. Artificial tendons were implanted either at the time of (immediate replacement, n=8), or four weeks after (delayed replacement, n=4) resection of the biological tendon. Hindlimb radiographs of the rabbits were obtained immediately after surgery and approximately every other week until the study endpoint (16 weeks post-surgery).ResultsAll suture anchors used for the tibialis cranialis artificial tendons remained secure and did not fail during the study. The suture linkage between the Achilles artificial tendon and anchor failed in 9 of 12 rabbits. In all cases, the mode of failure was suture breakage distant from the knot. Based on radiographic analysis, the mean estimated failure timepoint was 5.3±2.3 weeks post-surgery, with a range of 2-10 weeks. Analysis of variance (ANOVA) tests revealed no significant effect of tendon implantation timing or suture size on either the timing or frequency of suture anchor failure.ConclusionBased on the mode of failure, suture mechanical properties, and suture anchor design, we suspect that the cause of failure was wear of the suture against the edges of the eyelet in the suture anchor post, which reduced the suture strength below in vivo loads. Suture anchor designs differed for the tibialis cranialis and did not fail during the period of study. Future studies are needed to optimize suture anchor mechanical performance under different loading conditions and suture anchor design features.
A novel VCP modulator, KUS121, attenuates atherosclerosis progression by maintaining intracellular ATP and mitigating ER stress in endothelial cells
Authors: Zou Fuquan , Baba Osamu , Horie Takahiro , Nakashima Yasuhiro , Tsuji Shuhei , Yamasaki Tomohiro , Otani Chiharu , Xu Sijia , Imanaka Miyako , Matsushita Kazuki , Suzuki Keita , Kume Eitaro , Kojima Hidenori , Qian Qiuxian , Kimura Kayo , Sowa Naoya , Kakizuka Akira , Ono Koh 
Published: May 2024

AbstractBackgroundEndoplasmic reticulum (ER) stress signaling pathways have pivotal roles in atherosclerosis progression. Recently, we have developed Kyoto University Substance (KUS) 121, which selectively inhibits ATPase activities of valosin-containing protein (VCP) and consequently saves intracellular ATP consumption and mitigates ER stress.Methods and ResultsWe assessed the efficacy of KUS121 against atherosclerosis by its daily injection intoApoe−/−mice fed with Western Diet (WD) for 8 weeks. Consequently, KUS121 treatment reduced atherosclerosis progression by approximately 40% in atherosclerotic plaques. Interestingly, we found that C/EBP homologous protein (CHOP), an established ER stress marker, was mainly expressed in plaque endothelium. Therefore, we assessed the action of KUS121 in endothelial cells using the human endothelial cell line (EA.hy926 cells). As a result, KUS121 prevented ER stress-induced apoptosis and downregulated the IRE1 (Inositol-requiring enzyme) α-associated inflammatory pathways. Consistent with these in vitro findings, KUS121 treatment also significantly reduced endothelial apoptosis assessed by TUNEL staining and inflammation examined by immunostaining of Nuclear factor kappa B (NF-κB) and Intercellular adhesion molecule (ICAM) 1 at plaque endothelium. We also demonstrated that KUS121 maintained ATP levels in EA.hy926 cells and atherosclerotic plaque lesions using the single-wavelength or the FRET-based fluorescent ATP sensor. Supplementation of intracellular ATP by Methyl pyruvate (MePyr) attenuated ER stress-induced apoptotic and inflammatory pathways in endothelial cells, which could be the main mechanism how KUS121 reduces ER stress.ConclusionsKUS121 can be a new therapeutic option for atherosclerotic diseases by maintaining intracellular ATP levels and attenuating ER stress-induced apoptosis and inflammation in plaque endothelium.
Impact of PIK3CA gain and PTEN loss on mantle cell lymphoma biology and sensitivity to targeted therapies
Authors: Bettazova Nardjas , Senavova Jana , Kupcova Kristyna , Sovilj Dana , Rajmonova Anezka , Andera Ladislav , Svobodova Karla , Berkova Adéla , Herman Vaclav , Zemanova Zuzana , Daumova Lenka , Dolníkova Alexandra , Davis R. Eric , Trneny Marek , Klener Pavel , Havranek Ondrej 
Published: May 2024

ABSTRACTBesides many other mutations in known cancer driver genes, mantle cell lymphoma (MCL) is characterized by recurrent genetic alterations of important regulators of the phosphoinositol-3-kinase (PI3K) cascade includingPIK3CAgains andPTENlosses. To evaluate the biological and functional consequences of these aberrations in MCL, we have introduced transgenic expression ofPIK3CA(PIK3CA UP) and performed knockout ofPTENgene (PTEN KO) in 5 MCL cell lines. The modified cell lines were tested for associated phenotypes including dependence on upstream B-cell receptor (BCR) signaling (by an additionalBCRknockout). PIK3CA overexpression decreased the dependence of the tested MCL on prosurvival signaling from BCR, decreased levels of oxidative phosphorylation, and increased resistance to 2-deoxy-glucose, a glycolysis inhibitor. Unchanged AKT phosphorylation status and unchanged sensitivity to a battery of PI3K inhibitors suggested thatPIK3CAgain might impact MCL cells in AKT independent manner.PTENKO was associated with a more distinct phenotype: AKT hyperphosphorylation and overactivation, increased resistance to multiple inhibitors (most of the tested PI3K inhibitors, BTK inhibitor ibrutinib, and BCL2 inhibitor venetoclax), increased glycolytic rates with resistance to 2-deoxy-glucose, and significantly decreased dependence on prosurvival BCR signaling. Our results suggest that the frequent aberrations of the PI3K pathway may rewire associated signaling with lower dependence on BCR signaling, better metabolic and hypoxic adaptation, and targeted therapy resistance in MCL.Key point 1PIK3CA gain and PTEN loss decrease the dependence of MCL cells on B-Cell Receptor Signaling and anti-apoptotic BCL2.Key point 2PIK3CA gain and PTEN loss lead to complex metabolic rewiring and increased survival of MCL cells under hypoxia.
Degradation determinants are abundant in human noncanonical proteins
Authors: Casola Claudio , Owoyemi Adekola , Vakirlis Nikolaos 
Published: May 2024

AbstractThe comprehensive characterization of human proteins, a key objective in contemporary biology, has been revolutionized by the identification of thousands of potential novel proteins through ribosome profiling and proteomics. Determining the physiological activity of these noncanonical proteins has proven difficult, because they are encoded by different types of coding regions and tend to share no sequence similarity with canonical polypeptides. Evidence from immunopeptidomic assays combined with a better understanding of the quality control of protein synthesis suggest that many noncanonical proteins may possess low stability in the cellular environment. Here, we tested this hypothesis by analyzing the frequency of multiple sequence features eliciting either proteasomal degradation or autophagy across 91,003 canonical (annotated) protein isoforms and 232,460 noncanonical proteins. Overall, noncanonical proteins were enriched for degradation-related features compared to all canonical proteins. Notably, degradation determinants were also enriched in canonical protein isoforms starting with a non-methionine amino acid. Analyses of original and shuffled sequences showed evidence of selective pressure either against or towards the accumulation of specific degradation signatures only in major isoforms of canonical proteins. However, stability was significantly higher in noncanonical proteins with evidence of phenotypic effects when knocked-out in cell lines. Notably, we found that the C-terminal tail hydrophobicity represents a reliable proxy for degradation propensity with potential applications in identifying functional noncanonical proteins. These findings underscore the critical role of degradation processes in regulating the half-life of noncanonical proteins and demonstrate the power of degradation-associated signatures in discriminating noncanonical genes likely to encode for biologically functional molecules.
Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding
Authors: Cortez Thainá , Torres André LQ , Guimarães Murilo , Pinheiro Henrique B , Cabral Marcelo , Zielinsky Gabriel , Pereira Camila M , de Castro Giovanni M , Guerreiro Luana TA , Americo Juliana A , do Amaral Danielle LAS , Rebelo Mauro F 
Published: May 2024

AbstractMonitoring biodiversity on a large scale, such as in hydropower reservoirs, poses scientific challenges. Conventional methods such as passive fishing gear are prone to various biases, while the utilization of environmental DNA (eDNA) metabarcoding has been restricted. Most eDNA studies have primarily focused on replicating results from traditional methods, which themselves have limitations regarding representativeness and bias. In our study, we employed eDNA metabarcoding with three markers (12SrRNA, COI, and 16SrRNA) to evaluate the biodiversity of an 800 km² reservoir. We utilized hydrodynamic modeling to determine water flow velocity and the water renewal ratio throughout the study area. Additionally, we conducted statistical comparisons – rarefaction curves and multivariate methods – among samples as an alternative approach to assess biodiversity representation. The eDNA identified taxa previously documented in the reservoir by traditional monitoring methods, as well as revealed 29 – nine fishes and 20 non-fish – previously unreported species. These results highlight the robustness of eDNA as a biodiversity monitoring technique. Our findings also indicated that by randomly sampling 30% of the original number of samples, we could effectively capture the same biodiversity. This approach enabled us to comprehend the reservoir’s biodiversity profile and propose a straightforward, cost-effective monitoring protocol for the future based on eDNA.
Quantum recurrences in the kicked top
Authors: Anand Amit , Davis Jack , Ghose Shohini 
Published: May 2024

The emergence of classical chaos from an underlying quantum mechanics remains a challenging question due to the differences between dynamics driven by Schrödinger's equation versus Newton's equations. We present an infinite family of purely quantum recurrences that are not present in the classical limit of a chaotic system. They take the form of stroboscopic unitary evolutions in the quantum kicked top that act as the identity after a finite number of kicks. These state-independent recurrences are present in all finite dimensions and depend on the strength of the chaoticity parameter of the top. We further discuss the relationship of these periodicities to the quantum kicked rotor dynamics and the phenomenon of quantum antiresonance.

Published by the American Physical Society
2024
Institutions and financial development: Comparative analysis of developed and developing economies
Authors: Lisbinski Fernanda Cigainski , Burnquist Heloisa Lee 
Published: May 2024

PurposeThis article aims to investigate how institutional characteristics affect the level of financial development of economies collectively and compare between developed and undeveloped economies.Design/methodology/approachA dynamic panel with 131 countries, including developed and developing ones, was utilized; the estimators of the generalized method of moments system (GMM system) model were selected because they have econometric characteristics more suitable for analysis, providing superior statistical precision compared to traditional linear estimation methods.FindingsThe results from the full panel suggest that concrete and well-defined institutions are important for financial development, confirming previous research, with a more limited scope than the present work.Research limitations/implicationsLimitations of this research include the availability of data for all countries worldwide, which would make the research broader and more complete.Originality/valueA panel of countries was used, divided into developed and developing countries, to analyze the impact of institutional variables on the financial development of these countries, which is one of the differentiators of this work. Another differentiator of this research is the presentation of estimates in six different configurations, with emphasis on the GMM system model in one and two steps, allowing for comparison between results.
Richard Nagel and the challenge of employee retention
Authors: Rosenberg Stuart 
Published: May 2024

Research methodology
Information was obtained in interviews with Richard Nagel in Winter/Spring 2022. This information was supplemented by material from secondary sources. The only information that was disguised were the real names for Bob Crater, Tim Landy, Jane Tolley and Mary Nagel.
The case was classroom tested in Summer 2022. The responses from students helped to shape the writing of the case.

Case overview/synopsis
Richard Nagel, the owner of the RE/MAX Elite real estate agency in Monmouth Beach, New Jersey, has just learned that one of his agents, Tim Landy, quit and left the industry. Tim was a young real estate agent and Richard had spent considerable time training him. Tim was motivated and he worked hard to prospect for business, but he showed that he was experiencing difficulty closing on his sales. Richard decided to recommend that Tim work with another agent, Bob Crater, as Bob was an experienced salesman but was not doing the up-front prospecting that Tim was doing. Richard suggested two different strategies to the two agents – a pairing up arrangement and peer-to-peer learning. The outcome that Richard envisioned was that both of the struggling salesmen would benefit from either of these strategies, but Bob refused to collaborate.
Tim’s quitting was characteristic of an ongoing problem with employee retention that Richard had been experiencing as a manager in recent years. This problem caused Richard to think about how he recruited his real estate agents, how he developed them through coaching and how he motivated them so that they would stay happy in their job and not leave. He recognized the importance of thoroughly examining his retention strategy within the next 12 months so that he could better manage the problem and strengthen the productivity of his real estate agency.

Complexity academic level
The case is intended for an undergraduate course in human resources management, as it deals directly with recruiting, coaching and retaining employees.
Impact of the Affordable Care Act on access to accredited facilities for cancer treatment
Authors: Sabik Lindsay M. , Kwon Youngmin , Drake Coleman , Yabes Jonathan , Bhattacharya Manisha , Sun Zhaojun , Bradley Cathy J. , Jacobs Bruce L. 
Published: May 2024

AbstractObjectiveTo examine differential changes in receipt of surgery at National Cancer Institute (NCI)‐designated comprehensive cancer centers (NCI‐CCC) and Commission on Cancer (CoC) accredited hospitals for patients with cancer more likely to be newly eligible for coverage under Affordable Care Act (ACA) insurance expansions, relative to those less likely to have been impacted by the ACA.Data Sources and Study SettingPennsylvania Cancer Registry (PCR) for 2010–2019 linked with discharge records from the Pennsylvania Health Care Cost Containment Council (PHC4).Study DesignOutcomes include whether cancer surgery was performed at an NCI‐CCC or a CoC‐accredited hospital. We conducted a difference‐in‐differences analysis, estimating linear probability models for each outcome that control for residence in a county with above median county‐level pre‐ACA uninsurance and the interaction between county‐level baseline uninsurance and cancer treatment post‐ACA to capture differential changes in access between those more and less likely to become newly eligible for insurance coverage (based on area‐level proxy). All models control for age, sex, race and ethnicity, cancer site and stage, census‐tract level urban/rural residence, Area Deprivation Index, and year‐ and county‐fixed effects.Data Collection/Extraction MethodsWe identified adults aged 26–64 in PCR with prostate, lung, or colorectal cancer who received cancer‐directed surgery and had a corresponding surgery discharge record in PHC4.Principal FindingsWe observe a differential increase in receiving care at an NCI‐CCC of 6.2 percentage points (95% CI: 2.6–9.8; baseline mean = 9.8%) among patients in high baseline uninsurance areas (p = 0.001). Our estimate of the differential change in care at the larger set of CoC hospitals is positive (3.9 percentage points [95% CI: −0.5‐8.2; baseline mean = 73.7%]) but not statistically significant (p = 0.079).ConclusionsOur findings suggest that insurance expansions under the ACA were associated with increased access to NCI‐CCCs.
An expanded investigation of alliance security free riding
Authors: Kim Wukki , Sandler Todd , Shimizu Hirofumi 
Published: May 2024

AbstractThis paper provides an expanded analysis of NATO security burden sharing by including a variety of conglomerate security terms that involve subsets of military expenditure (ME), UN and non‐UN peacekeeping contributions, global health spending, UN environmental support, and official development assistance. In so doing, we identify components of security spending that promote or inhibit free riding on allies' security spillovers. Additionally, we examine security burden sharing when the NATO alliance is conceptually augmented to include three key Asia‐Pacific allies – Australia, Japan, and the Republic of Korea. The paper's statistical tests for security burden sharing rely on spatial‐lag panel models that account for ally connectiveness based on alliance membership, contiguity and US power projection, and allies’ relative locations. Security subsets containing ME display robust free riding or reliance on other allies’ security spillovers, while security subsets not containing ME indicate allies responding positively to security spillovers.
Comparison of SP263 and 22C3 pharmDx assays to test programmed death ligand‐1 (PD‐L1) expression in surgically resected non‐small cell lung cancer
Authors: Shigeta Naoko , Murakami Shuji , Yokose Tomoyuki , Isaka Tetsuya , Shinada Kanako , Nagashima Takuya , Adachi Hiroyuki , Shigefuku Shunsuke , Murakami Kotaro , Miura Jun , Kikunishi Noritake , Watabe Kozue , Saito Haruhiro , Ito Hiroyuki 
Published: May 2024

AbstractBackgroundAtezolizumab, one of the immune checkpoint inhibitors, has been approved as an adjuvant treatment following resection and platinum‐based chemotherapy in patients with stage II–IIIA non‐small cell lung cancer with 1% or more programmed death ligand‐1 (PD‐L1) expression. The Food and Drug Administration (FDA) has approved SP263 as a companion diagnostic assay for adjuvant treatment with atezolizumab; however, in clinical practice, the 22C3 assay is most commonly used for advanced non‐small cell lung cancer. Therefore, our study aimed to compare two PD‐L1 assays, SP263 and 22C3, to evaluate whether 22C3 could replace SP263 when deciding whether to administer adjuvant atezolizumab.MethodsWe retrospectively and prospectively analyzed 98 patients who underwent surgical resection at Kanagawa Cancer Center (Japan). An immunohistochemistry assay was performed for all the cases with both SP263 and 22C3. We statistically analyzed the concordance of PD‐L1 expression between SP263 and 22C3 assays.ResultsThe concordance between the two assays using Cohen's kappa was κ = 0.670 (95% CI: 0.522–0.818) at the 1% cutoff and κ = 0.796 (95% CI: 0.639–0.954) at the 50% cutoff. The Spearman correlation coefficient of 0.874 (p < 0.01) indicated high concordance. PD‐L1 expression with 22C3 resulted slightly higher than that with SP263.ConclusionsThis study showed a high concordance of PD‐L1 expression with the SP263 and 22C3 assays. Further studies examining the therapeutic effects of adjuvant atezolizumab are required.