bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistological Diagnosis of Rabbit Haemorrhagic Disease in Experimentally Infected Rabbits in Spain
Authors: Carrasco L. , Rodriguez F. , de las Mulas J. Martin , Wohlsein P. , Fernandez A. 
Published: May 2010

SummaryThe immunoreactivity of routinely processed liver and lung tissue samples obtained from rabbits inoculated with tissue explants from naturally infected animals when antisera directed against parvovirus from different species (canine, feline and porcine) as well as a RHD virus antiserum were employed has been tested by different immunoperoxidase methods. Cross‐reactivity between RHD‐virus antigens and parvovirus antigens was present. Best results were obtained with RHD and canine parvovirus antisera with the ABC method.The immunoreactivity in the liver was found in hepatocytes, Kupffer and bile duct cells. In the lung, it was exclusively observed in intravascular macrophages.
Clinical Selection Criteria for Secondary Examinations of the Health‐Status of Broiler Flocks*
Authors: Behr K.‐P. , Siegmann O. , Hinz K.‐H. , Erhorn Ingrid , Salisch H. , Friederichs Maren , Weiss H. , Arndi Gisela 
Published: May 2010

SummaryResults of post‐mortem and laboratory examinations of broilers sampled randomly or selected by clinical symptoms were compared. The male: female‐ratio was in all cases nearly equal for randomly sampled and selected birds. Regarding post‐mortem findings, differences dependent on selection criteria were observed: in randomly‐sampled birds the frequency of breast‐blisters and plantar inflammations was higher than in selected broilers, whereas in the latter osteodystrophy and bursal atrophy were seen more frequently. Alterations of joints and tendon‐sheaths caused by a reovirus‐arthritis in one flock were seen in same frequency in randomly sampled and selected birds, respectively. Parasitological examinations revealed the highest coccidial lesion scores in selected birds with lameness or slightly retarded growth but when dwarfish birds were included into calculations, there was no significant difference among both groups. Results of bacteriological and serological examinations showed no differences between the groups.
Kinetik der Inaktivierung von Salmonellen bei der thermischen Desinfektion von Flüssigmist
Authors: Soldierer W. , Strauch D. 
Published: May 2010

SummaryKinetics of Death of Salmonellae During Thermal Liquid Manure DisinfectionTechnical processes for thermal liquid manure disinfection usually reach temperatures between 50°C and 70°C. The destruction of important infectious micro‐organisms can be expected in this temperature range. The purpose of the investigations reported here was to study the thermal resistance of Salmonellae during heat treatment of liquid manure. Salmonella senftenberg 775W survived much longer than each of 12 other strains from 8 different Salmonella serovars. Resulting from a regression analysis kinetics of thermal death were determined for this strain and decimal reduction times were calculated in cases of an exponential die‐off. D60‐values ranged from 47 to 138 sec depending on the type of slurry used. Further investigations on the inactivation of Salmonella senftenberg 775W at 50°C, 55°C, 60°C, and 65°C were carried out and following D‐values were obtained: D50 = 56.7 min, D55 = 11.5 min, D60 = 2.3 min, D65 = 0.47 min. The resulting ***ZD‐value was 7.2°C.Minimum requirements concerning temperature and heating time can be derived from the results of this study. The given recommendations may only be applied, if technical processes work without any functional deficiencies and thermal energy is evenly distributed in the heated slurry. Combinations of temperature and heating time should not fall below the following values: 50°C / 15 h, 55°C / 3 h, 60°C / 30 min, 65°C / 5 min.ZusammenfassungTechnische Verfahren zur thermischen Entseuchung von Flüssigmist arbeiten meist bei Temperaturen zwischen 50°C und 70°C. Im Rahmen der vorliegenden Untersuchung wurde das Absterbeverhalten von Salmonellen bei der Hitzebehandlung von Flüssigmist in diesem Temperaturbereich untersucht.Für Salmonella senftenberg 775W, dem hitzeresistentesten von 13 untersuchten Stämmen aus 9 verschiedenen Serovaren dieser Spezies, wurden in Flüssigmist verschiedener Herkunft D60‐Werte von 47–138 Sekunden ermittelt. Weitere Inaktivierungsversuche bei 50°C, 55°C, 60°C und 65°C ergaben für Salmonella senftenberg 775W einen z‐Wert von 7,2°C und folgende D‐Werte: D50 = 56,7, min, D55 = 11,5 min, D60 = 2,3 min, D65 = 0,47 min.Ausgehend von den Ergebnissen dieser Untersuchungen werden Mindestanforderungen für die thermische Desinfektion von Flüssigmist abgeleitet. Voraussetzung für die Anwendung dieser Maß‐stäbe ist allerdings der einwandfreie Funktionsablauf aller technischen Einrichtungen der betreffenden Anlagen, der eine gleichmäßige Wärmeeinwirkung garantieren muß. Folgende Kombinationen von Temperatur und Heißhaltezeit sollten nicht unterschritten werden: 50°C/15h, 55°C/3h, 60°C/ 30 min, 65°C / 5 min.
Characterization of 2 Groups of Actinomyces‐like Bacteria Isolated from Purulent Lesions in Pigs
Authors: Hommez J. , Devriese L. A. , Miry C. , Castryck F. 
Published: May 2010

SummaryTwo groups of Actinomyces‐like bacteria isolated from purulent lesions in pigs, were characterized and compared with Actinomyces pyogenes of porcine origin. One group showed characteristics which differed in many respects from A. pyogenes but which corresponded more closely to those of the non‐official species ‘Actinomyces suis’. Certain carbohydrate reactions differed from those given in earlier descriptions. This group was isolated only from purulent vaginal discharge and aborted foeti. The other group resembled the ‘A. suis’‐like bacteria but differed in its colony morphology, hippurate and to some extent also in its cellobiose and gluconate reactions and in its habitat.It was concluded that ‘A. suis’ may occur in lesions other than mammary actinomycosis in areas where this disease is extremely rare, and that its natural habitat is the genital tract of pigs. Furthermore a probably related group exists, which is found in purulent lesions located mainly outside the porcine genital tract. These organisms appear to be as frequent in these types of lesions as A. pyogenes, with whom they can easily be confused in routine diagnostic bacteriology.
Hämorrhagische Septikämie der Kaninchen (Rabbit Haemorrhagic Disease, RHD) ‐ Untersuchungen zum quantitativen Antigennachweis
Authors: Liebermann Ht. , Bergmann H. , Lange Elke , Schirrmeier H. 
Published: May 2010

SummaryRabbit Haemorrhagic Disease (RHD) ‐ investigation for quantification of antigenThe efficiency of a vaccine of inactivated virus is influenced to a great deal by the mass of antigen per dose of application. Therefore it is essential to know the concentration of the antigen. We tested a physical method for quantification of the RHD‐Virus. It implies a centrifugation of the prepurified, if necessary, preconcentrated infectious or inactivated virus in a gentle sucrose gradient. It is followed by analysis in a sensitive UV flow‐through photometer with a computer calculated virus mass. The extinction coefficient (optical density) of the virus (175S‐component) at 254 nm is 3.9cm2/mg. The haemagglutination test and the ELISA were used to prove the virus specificity of the optical peak and partial for comparing them with the method mentioned above.ZusammenfassungDie Wirksamkeit einer Vakzine aus inaktiviertem Virus wird in entscheidendem Maße von der Antigenmenge pro Impfdosis bestimmt. Daher ist es wichtig, die Konzentration des Antigens zu kennen. Es wurde eine physikalische Viruskonzentrationsbestimmungsmethode für das HSK‐Virus erprobt. Sie beinhaltet eine Zentrifugation im flachen Saccharosedichtegradienten von (vorgereinigtem) nötigenfalls vorkonzentriertem infektiösem oder inaktiviertem Virus mit anschließender Analyse im nachweisempfindlichen UV‐Durchflußphotometer bei computergestützter Auswertung.Der Extinktionskoeffizient des Virus (175 S‐Komponente) bei 254 nm liegt bei 3,9 cm2/mg.Zum Nachweis der Virusspezifitat der optischen Peaks und z.T. vergleichend wurden der Hämagglutinationstest und der ELISA eingesetzt.
Translation of Double‐Stranded RNA Segments 1 and 2 of Chuzan Virus in Rabbit Reticulocyte Lysates
Authors: Yoshida Tatsumi , Harasawa Ryô , Goto Yoshiyuki 
Published: May 2010

SummaryCoding assignments of genome segments 1 and 2 of Chuzan virus strain K‐47 were studiedin vitroby using rabbit reticulocyte lysates. The double‐stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)‐polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [35S]methionine were resolved by SDS‐polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.
The Helminth Fauna from the Common Seal (Phoca vitulina vitulina, Linné, 1758) of the Wadden Sea in Lower Saxony*
Authors: Claussen D. , Strauss V. , Ising Susanne , Jäger Martina , Schnieder T. , Stoye M. 
Published: May 2010

SummaryDuring the seal epidemic in 1988 and the beginning of 1989, 115 common seals found dead on the shores of the Wadden Sea of Lower Saxony were investigated for the presence of nematodes. The lungworm Otostrongylus circumlitus Railliet 1899 was found in 26.1 % of the seals, the lungworm Parafilaroides gymnurus Railliet 1899 in 26.9 % and the heartworm Dipetalonema spirocauda Leidy 1858 in 32.2 % of the seals. In the digestive tract, two anisakid species were found, Pseudoterranova decipiens Mozgovoi 1951 in 87.8% and Contracaecum osculatum Rudolphi 1802 in 10.4% of the seals. Statistical analysis revealed a negative correlation between age of the seals and prevalence and intensity of infestation with the two lungworm species and the heartworms. Adult seals were found not to be infected with Dipetalonema spirocauda and Parafilaroides gymnurus, whereas the prevalence of the two anisakid species increased with increasing age of the seals. Pseudoterranova decipiens was found more often in autumn and winter than in summer. This parasite was more prevalent and had higher worm counts in the eastern part of the Wadden Sea than in the western part. Parafilaroides gymnurus and Dipetalonema spirocauda were found more often in seals with smaller blubber thickness than in well fed seals.
Pathological Studies on Central Nervous Tissues of Rats Infected with Rattus Serotype Hantavirus (SR‐11 Strain)
Authors: Mori F. , Kobayashi K. , Itakura C. , Arikawa J. , Hashimoto N. 
Published: May 2010

SummaryNewborn rats inoculated intraperitoneally with a hantavirus strain (SR‐11) developed neurological signs such as ataxia and limb paralysis. The main histological lesions were scattered and multiple neuronal degeneration and necrosis with eosinophilic cytoplasmic inclusion bodies (CIB) in the brain, spinal cord and ganglia. Immunohistochemically, the viral antigen was detected in neurons, capillary endothelial and glial cells throughout the nervous tissue and CIB were identified as consisting of hantaviral antigen (nucleocapsid protein). Ultrastructurally, CIB in the neurons consisted of an accumulation of granular or filamentous materials, or both. They were seen near a well‐developed Golgi apparatus and associated with well‐developed rough endoplasmic reticulum and increased numbers of ribosomes. The present results suggested that this virus strain was highly infective in neurons of the newborn rats and that excess production of viral antigen which accumulated as CIB resulted in the neuronal changes.
Assessment of Lymphocyte and Phagocytic Functions in Goats treated with Glucan
Authors: Benda V. , Mádr P. 
Published: May 2010

SummaryThe effect of glucan, a biological response modifier of yeast origin, on different immune functions was studied after the intramuscular application in goats. The simultaneous administration of glucan with human serum albumin or tetanus toxoid significantly stimulated the antibody production to both antigens differing in their thymus dependency. Similarly, the phagocytizing activity of the blood polymorphonuclear leukocytes measured by the reductase colorimetric assay significantly increased one week after the glucan treatment. However, suppression of T‐lymphocyte function in experimental animals was determined by the lymphocyte transformation test particularly in response to phytohemagglutinin and concanavalin A. The results of the study indicate that glucan can modulate some elements of the ruminant immune response.
Inhibitory Effect of Sulfamonomethoxine on Capsule Formation of Bordetella bronchiseptica
Authors: Kuwano A. 
Published: May 2010

SummaryBordetella bronchiseptica phase I organism possesses a capsule and has the ability to agglutinate with K antiserum, although phase III organism lacks both. The present study examines the effect of sulfamonomethoxine (SMMX) on capsule formation of B. bronchiseptica. I also investigated whether or not the organisms possessed a capsule by bacterial agglutination with K antiserum. Three SMMX‐resistant strains of B. bronchiseptica phase I organisms showed loss of agglutinability with K antiserum by culturing them at a higher concentration of 1.56μg/ml of SMMX.These results indicated that capsule formation of SMMX‐resistant B. bronchiseptica is inhibited by SMMX.
Immunohistological Detection of Orthopox Virus Antigen
Authors: Minkus G. , Czerny C.‐P. , Hermanns W. 
Published: May 2010

SummaryThis paper describes the immunohistochemical staining of orthopox virus (OPV) antigen in liver tissue of experimentally infected mice and in cell cultures. Using two different monoclonal antibodies, virus detection was achieved in cryostat‐, but not in paraffin‐sections.
InvitroBinding ofPasteurella multocidaCell Wall Preparations to Tracheal Mucus of Cattle and Swine and to a Tracheal Epithel Cell Wall Preparation of Cattle
Authors: Bötcher L. , Lübke A. , Hellmann E. 
Published: May 2010

SummaryOuter membrane preparations of variousPasteurellaisolates (Pasteurella multocidaand some otherPasteurellaspecies) from cattle and swine were extracted by N‐lauryl‐sarcosine sodium salt. Capsular extracts were prepared by heat treatment. Both preparations bound to epithel cell wall preparations (ECW) of trachea from cattle and to tracheal mucus of cattle and swine. Binding was demonstrated by an enzyme‐linked immunosorbent assay (ELISA). Distinct high adherence values were shown by the greater part of membrane preparations of mucoidPasteurellastrains, especially when originating from cattle.
Antibiotic Resistance Patterns and Pigment Production of Streptococci of Serological Group B Isolated from Bovines and Humans
Authors: Wibawan I. W. T. , Lautrou Y. , Lammler Ch. 
Published: May 2010

SummaryAll 127 streptococci of serological group B isolated from bovines and humans were resistant to bacitracin (0.04 U) and gentamicin and susceptible to penicillin. Of the bovine isolates examined 10 %, 8 %, 22 %, 2 % and 30 % were resistant to bacitracin (10 U), cefoxitin, clindamycin, erythromycin and tetracycline respectively. Of the human isolates 52%, 21 %, 38%, 3% and 65% were resistant to bacitracin (10 U), cefoxitin, clindamycin, erythromycin and tetracycline respectively. Most of the bovine strains were susceptible, most of the human strains were resistant to bacitracin and tetracycline. A relation of tetracycline resistance to group B streptococcal microcapsule was proposed. Pigmentation studies revealed generally a weak pigmentation of bovine and a strong pigmentation of human group B streptococci. Antibiotic resistance patterns together with the ability to form a yellow to orange‐red pigment might be used for characterization of individual cultures of group B streptococci.
Biochemical and Serological Properties of Streptococcus uberis
Authors: Lämmler C. 
Published: May 2010

SummaryThe Strep‐Zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of Streptococcus uberis. The biochemical profiles of the S. uberis cultures clearly differed from those of S. agalactiae and S. dysgalactiae. Serological grouping of S. uberis revealed polysaccharide antigens of groups E, G, P and U. Some cultures of S. uberis demonstrated CAMP‐like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of Helix pomatia and Dolichos biflorus. The occurrence of group polysaccharides, CAMP‐like reactivities, and the lectin agglutination reactions were obviously not related to each other or to any of the biochemical properties. These reactions, possibly of importance as virulence factors, might serve as epidemiological markers.
Etiology of Rabbit Haemorrhagic Disease Spontaneously Occurring in Korea
Authors: Park J.H. , Kida H. , Ueda K. , Ochiai K. , Goryo M. , Itakura C. 
Published: May 2010

SummaryCausative agent of rabbit haemorrhagic disease (RHD) was purified by CsCl density gradient centrifugation from the liver homogenate of rabbits infected with RHD virus which originated from Korea. The viral particles were 35–40 nm in diameter, and had hollow depressions on their surface. Protein A‐gold immunoelectron microscopy clearly showed that the convalescent antisera of diseased rabbits reacted specifically with the virus particles. SDS‐PAGE and Western blot analyses demonstrated that the structural protein of the virus was composed of a single major polypeptide of 63 kD. These findings indicate that the causative agent of RHD, tentatively named as picornavirus in Korea, belongs to calicivirus.
Cerebrospinal Elaphostrongylosis in Sheep in Northern Norway
Authors: Handeland K. 
Published: May 2010

SummaryPathologic examination of four sheep from northern Norway exhibiting neurologic signs of paresis, paralysis and vestibular system disease revealed nematodes in the central nervous system (CNS). The worms were identified as Elaphostrongylus rangiferi Mitskevich, 1960, the elaphostrongylid nematode of reindeer (Rangifer tarandus tarandus).Microscopic lesions found in the CNS were focal traumatic encephalomyelomalacia caused by migrating worms, eosinophilic meningitis and choroiditis, lymphohistiocytic and nematode granulomas, and perineural infiltrations.The disease occurred in November and December 1990, after the sheep had been on pasture in areas frequented by considerable numbers of reindeer.
Antibiotic Susceptibility Testing of Mycoplasma bovis using Tween 80 Hydrolysis as an Indicator of Growth
Authors: Devriese L. A. , Haesebrouck F. 
Published: May 2010

SummaryLysis and precipitation of polyoxyethylene sorbitan monoleate (Tween 80) was used as an indicator of growth in quantitative antibiotic dilution susceptibility tests in microtiter plates. This allowed test results to be read after 2 or 3 days. Antibiotic sensitivity levels obtained with this method were homogenous and tightly clustered. No acquired resistance was found among 16 field strains isolated in 1990.
Pathomorphological and Immunohistological Findings in Progeny of Goats Experimentally Infected with Pestiviruses
Authors: Wohlsein P. , Trautwein G. , Depner K. R. , Hübschle O.J.B. , Liess B. 
Published: May 2010

SummaryA total of 25 pregnant goats without neutralizing antibodies against BVD virus were inoculated with two different pestivirus isolates at eight different stages of gestation. In both infection groups, various malformations were observed in fetuses and neonates. In three twins with neutralizing antibodies against BVD virus leucoencephalomalacia occurred, characterized by gelatinous transformation in the cerebral hemispheres. These lesions were comparable to alterations described in alternative pathology of Border disease in sheep. Although the immunohistological findings are characteristic for immunological tolerance and viral persistence, viable offspring persistently infected with pestivirus was not observed.
Properties of Nine Continuous B‐Cell Lines Established from Enzootic Bovine Leukosis Tumors
Authors: Koyama Hiroyuki , Hohdatsu Tsutomu , Satake Minoru , Kobayashi Masaki , Ashizawa Takayoshi , Sugimoto Kaoru , Yoshikawa Hiroyasu , Okada Kosuke , Yoshikawa Takashi , Saito Hiroshi 
Published: May 2010

SummaryWe established 9 cell lines from 63 tumor cases of enzootic bovine leukosis and studied their properties. Cells of all lines formed small clumps and floated in culture medium, indicating growth. Four of the 9 cell lines were surface immunoglobulin (SIg)‐positive, but the remaining 5 line cells were negative for SIg or, if SIg was detected, the percentage of SIg‐positive cells was very low. Tests for the properties of the cells with monoclonal antibodies to lymphocytes revealed that the established line cells are B‐lymphocytes. Morphological observation also revealed that they had the morphology of B‐lymphoblastic cell. The results of E and EAC rosette assay were negative, but 6 of 8 cell lines were positive for EA rosetting. All the 9 cell lines reacted with MoAb C‐143, which recognizes the tumor‐associated antigen (TAA) of the EBL tumor cell. All 9 cell lines produced bovine leukosis virus (BLV). These results suggest that the 9 cell lines are tumor cells derived from B‐lymphocytes of EBL.
Lytic Activities, Protein Profiles and Morphologic Characteristics of New Bacteriophages Isolated from Canine and Human Staphylococcus aureus Strains
Authors: Adesiyun A. A. , Viebahn A. , Sahl H. G. , Lenz W. , Schaal K. P. 
Published: May 2010

SummaryThe lytic activity, protein profile and morphology of five newly isolated phages from canine Staphylococcus aureus strains and one from a human S. aureus strain were compared with those of selected phages in the international phage sets (IPS). Five canine phages lysed 57 (76.0 %) of 75 canine isolates of Staphylococcus aureus from Nigeria at routine test dilution (RTD) while 34 (IPS) phages typed only 31 (41.3 %) strains at RTD or/and 100‐RTD. The new human phage lysed 11 (14.7%) of 75 strains isolated from human diarrhoea. The new phages were readily propagated, specific in activity and stable during storage at 4°C.Prominent proteins detected by SDS‐PAGE indicated similarities between some of the phages but one canine phage was distinctly different, as was its morphology which was an isometric head with a short tail compared to oval heads and long tails which characterized others. IPS phages in the same serologic group had similar protein profiles but no correlation was observed with lytic groups. The use of protein profile and electron micrographs allowed classification of the phages into serogroups.It is concluded that the newly isolated canine phages could be very useful in typing Nigerian canine strains of S. aureus.
Prevalence of Feline Leukemia Virus and Antibodies to Feline Immunodeficiency Virus in Cats in Norway
Authors: Ueland K. , Lutz H. 
Published: May 2010

SummarySerum samples from 224 Norwegian cats were analyzed for the presence of feline leukemia virus (FeLV) p27 common core antigen, and for antibodies to feline immunodeficiency virus (FIV). Ninety specimens originated from the serum bank at the central referral clinic at the Norwegian College of Veterinary Medicine, which had been collected during the years 1983–1989; 67 sera were submitted from veterinarian practitioners; while 67 sera originated from cats presented for euthanasia. The cats were classified into one “healthy” and one “sick” group.Only 2.2 % of sick cats and 1.2 % of healthy cats showed FeLV antigenemia, a finding which is lower than which has been reported from many other countries.The prevalence of FIV antibodies was 10.1 % in sick cats and 5.9 % in healthy cats. Antibodies to FIV was most prevalent in male cats (14.7%) than in female cats (2.1 %), and more prevalent among domestic cats (12.0 %) compared to pedigree cats (2.4 %). Antibodies to FIV in the cats demonstrated increasing prevalence with increasing age. It may be concluded that FeLV causes minor problems in Norwegian cats, while FIV is present in a similar prevalence to what is reported form other countries.