bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a dual affinity tagged allele of the Rb1 tumor suppressor gene in the mouse
Authors: Xiao Hai , Zhang Xiaojing , Goodrich David W. 
Published: December 2009

AbstractLoss of Rb1 tumor suppressor gene function is involved in the genesis of most human cancers. Novel therapies targeting Rb1 have been slow to develop because of our incomplete understanding of its molecular mechanisms of action. Rb1 protein (pRb) binds a host of cellular genes and proteins, and these molecular interactions mediate its various functions. Given the potential complexity of these molecular interactions and the lack of established methods for pRb purification, it has been difficult to systematically identify gene and protein interactions relevant to tumor suppression in different tissues in vivo. To address this limitation, we have generated a dual affinity tagged Rb1 allele in the mouse. The tagged allele functions as wild type and the encoded protein can be purified by tandem affinity chromatography. This allele will facilitate identification and characterization of native pRb molecular interactions in any tissue accessible in the mouse. genesis 48:121–126, 2010. © 2009 Wiley‐Liss, Inc.
Pyrene‐Functionalised Copper Complexes as Potential Dual‐Modality Imaging Agents (Eur. J. Inorg. Chem. 1/2010)
Authors: Holland Jason P. , Fisher Victoria , Hickin Jennie A. , Peach Josephine M. 
Published: December 2009

AbstractThe cover picture shows the structure of a pyrene‐functionalised bis(thiosemicarbazonato)copper complex that has been designed as a first‐generation dual‐modality agent for combination with in vivo positron emission tomography and in vitro fluorescence microscopy. Details of the synthesis as well as characterisation of the electronic properties by using spectroelectrochemistry, fluorescence emission spectroscopy and density functional calculations are reported in the article by J. P. Holland, J. M. Peach et al. on p. 48 ff.
Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor
Authors: Santiago‐Rodríguez Lenibel , Sánchez‐Pomales Germarie , Cabrera Carlos R. 
Published: December 2009

AbstractA gold surface modified with a self‐assembled monolayer of 11‐amino‐1‐undecanethiol (AUT) was used for the covalent immobilization of oxidized single‐walled carbon nanotubes (SWNTs). The as‐described SWNTs‐modified substrate was subsequently used to attach single‐stranded deoxyribonucleic acid (ssDNA) used as a substrate for DNA hybridization. Electrochemical impedance spectroscopy measurements were performed to follow the DNA hybridization process by using the redox couple [Fe(CN)6]3−/4− as a marker ion. Specifically, changes in charge transfer resistance obtained from the Nyquist plots were used as the sensing parameter of DNA hybridization. The substrate sensitivity towards changes in target DNA concentration, its selectivity toward different DNA sequences and its reusability are successfully demonstrated in this report.
Investigation of DOM and THMFP in two reservoir waters of Changchun (China)
Authors: Lin Ying‐Zi , Yin Jun , Zhao Ke , Wang Jian‐Hui 
Published: December 2009

AbstractThe two reservoir water samples from Xinlicheng (SW1) and Shitoukoumen (SW2) were collected to investigate the characterization of dissolved organic matter (DOM) and trihalomethanes formation potential (THMFP). The six organic fractions were, hydrophilic acid fraction (HPIA), hydrophilic base fraction (HPIB), hydrophilic neutral fraction (HPIN), hydrophobic acid fraction (HPOA), hydrophobic base fraction (HPOB), and hydrophobic neutral fraction (HPON), separated with the XAD‐16, AG‐MP‐50, and WA‐10 resins. The DOM chemical composition sequences and specific THMFP of the six organic fractions in two raw waters are different. The experimental results show that HPOB in SW1 and HPOA in SW2 are the main precursor of THMFP among the six organic fractions. However, HPIB exhibits the highest chlorination activity in forming THMs. It is also found that the value of FI/DOC and the THMFP have better positive correlation. Compared with former results, a part of these findings are different from some reports. It is implied that certain sourcewater has a unique nature of DOM and DBPs. © 2009 American Institute of Chemical Engineers Environ Prog, 2010
Simplified inelastic seismic analysis of base‐isolated structures using the N2 method
Authors: Kilar Vojko , Koren David 
Published: December 2009

AbstractIn the paper a simplified nonlinear method has been applied to the analysis of base‐isolated structures. In the first part, a three‐linear idealization of the capacity curve is proposed. The initial stiffness is defined based on the first yielding point in the superstructure, whereas the secondary slope depends on the failure mechanism of the superstructure. A consequence is a much more pronounced secondary slope, which does not correspond to the presumptions used in the originally proposed N2 method. A parametric nonlinear dynamic study of single degree of freedom systems with different hardening slopes and damping has been performed for an ensemble of seven EC8 spectrum‐compatible artificial accelerograms. It was concluded that, in the long‐period range, the equal displacement rule could be assumed also for the proposed systems with non‐zero post‐yield stiffness. In the second part, the proposed idealization was used for the analysis of isolated RC frame buildings that were isolated with different (lead) rubber‐bearing isolation systems. The stiffness of the isolators was selected for three different
protection levels and for three different ground motion intensities, which have resulted in elastic as well as moderately and fully damaged superstructure performance levels. Three different lateral load distributions were investigated. It was observed that a triangular distribution, with an additional force at the base, works best in the majority of practical cases. It was concluded that the N2 method can, in general, provide a reasonably accurate prediction of the actual top displacement, as well as of the expected damage to the superstructure. Copyright © 2009 John Wiley & Sons, Ltd.
A physical model for dynamic analysis of wine barrel stacks
Authors: Candia Gabriel , de la Llera Juan C. , Almazán José Luis 
Published: December 2009

AbstractThis paper deals with the earthquake response of wine barrel stacks using a physical model of rigid‐body components with discrete flexible and damped contact elements. An analytical 3D formulation of the complex dynamic behavior of different barrel stack configurations is presented. Such behavior includes the real geometry of the bodies, large displacements and rotations, the use of non‐linear contact elements to account for impact and sliding between bodies, and the resulting local energy dissipation at contact. The parameters defining the physical and mathematical model were calibrated experimentally, and the dynamic behavior of a benchmark barrel stack configuration was compared with the experimental results obtained from the literature. It was found that the model is able to predict the exact mode of collapse and the overall behavior of the system. Copyright © 2010 John Wiley & Sons, Ltd.
Effect of Various Hydrophobic Concentrations and Base Weights of Gas Diffusion Layer for Proton Exchange Membrane Fuel Cells
Authors: Lin J.‐H. , Ko T.‐H. , Kuo W.‐S. , Lin Y.‐H. , Huang C.‐C. , Chen W.‐C. 
Published: December 2009

AbstractThis study investigates the gas permeability, conductivity and performance of two types of gas diffusion layer (90 g m–2 and 190 g m–2) with various hydrophobic treatments. The performance is measured using a single proton exchange membrane fuel cell (PEMFC) with an active area of 25 cm2. The results prove that 90 g m–2 carbon paper has the best current density in 5% hydrophobic concentration. The polarisation curves of fuel cell were plotted by similar operating conditions with different micro‐porous layers (MPLs) on carbon papers surface. These results provide a wide choice of hydrophobic agents. These results concerning the balance between base weights and performance provide important information for the fabrication of stacks and support for industrial applications.
Outcome after surveillance of low-grade and indefinite dysplasia in patients with ulcerative colitis
Authors: Pekow Joel R. , Hetzel Jeremy T. , Rothe Jami A. , Hanauer Stephen B. , Turner Jerrold R. , Hart John , Noffsinger Amy , Huo Dezheng , Rubin David T. 
Published: December 2009

Abstract

Background
The management of low-grade (LGD) and indefinite dysplasia (IND) in patients with ulcerative colitis (UC) remains controversial, as outcomes after a diagnosis of LGD or IND in previous studies vary widely.

Methods
All patients evaluated were from a single institution referral center who had a history of UC and a diagnosis of either LGD or IND between 1994 and 2008 as confirmed by 2 expert gastrointestinal (GI) pathologists. Data were collected by chart review of electronic and paper medical records. All patients who did not undergo a colectomy within 90 days of their dysplasia diagnosis were included in the final analysis. Hazard ratios for risk factors as well as incidence rates and Kaplan–Meier estimates were used to calculate the progression to high-grade dysplasia (HGD) or colorectal cancer (CRC).

Results
Thirty-five patients were included in the analysis, of whom 2 patients with IND and 2 patients with LGD developed HGD or CRC over a mean duration of 49.8 months. In total, the incident rate for advanced neoplasia for all patients was 2.7 cases of HGD or CRC per 100 person-years at risk. For flat and polypoid LGD the incident rate of advanced neoplasia was 4.3 and 1.5 cases per 100 person-years at risk, respectively. Patients with primary sclerosing cholangitis (PSC) had an incident rate of 10.5 cases per 100 years of patient follow-up.

Conclusions
We report a low rate of progression to HGD or CRC in patients who underwent surveillance for LGD or IND; polypoid dysplasia showed less risk of progression than flat dysplasia. (Inflamm Bowel Dis 2010)
Retraction: Human monoclonal antibody GNX‐8 directed to extended type 1 chain: specific binding to human colorectal cancer
Authors: Yang Mei‐Chun , Hsu Feng‐Lin , Handa Kazuko , Hakomori Senitiroh , Lee Mei‐Hsien , Liu Liahng‐Yirn , Chang Shu‐Yen , Ting Jerry , Wen Jaw‐Yuan , Ishida Isao , Chang Tong‐Hsuan 
Published: December 2009

The above article, published online on 21 December 2009 in Wiley Online Library (wileyonlinelibrary.com), has been withdrawn by agreement between the journal Editor in Chief, Prof. Peter Lichter, and John Wiley & Sons Ltd. The withdrawal has been agreed due to the authors having requested that the paper not be officially published until a patent issue had been resolved. Although many attempts were made to contact the authors, no further information was received. We are therefore withdrawing the article.Reference

Yang M‐C,
Hsu F‐L,
Handa K,
Hakomori S,
Lee M‐H,
Liu L‐Y,
Chang S‐Y,
Ting J,
Wen J‐Y,
Ishida I,
Chang T‐H. . Int. J. Cancer. 2009. Accepted Author Manuscript. doi: 10.1002/ijc.25131.
Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient
Authors: Swoboda Rolf K. , Somasundaram Rajasekharan , Caputo Laura , Berencsi Klara , von Franzke Paul , Taylor Douglas D. , Marincola Francesco M. , Meropol Neal J. , Sigurdson Elin , Miller Eric , Herlyn Dorothee 
Published: December 2009

AbstractImmunotherapy of colorectal carcinoma (CRC) has great promise as the presence of T lymphocytes in CRC tissues in situ is correlated with reduced recurrence and increased survival. Thus, identification of the antigens recognized by T cells of CRC patients may permit development of vaccines with potential benefit for these patients. Using expression cloning, we identified the antigen, nucleophosmin (Npm), recognized by an HLA‐A1 restricted cytotoxic T lymphocyte (CTL) line derived from the peripheral blood mononuclear cells (PBMC) of a rectal cancer patient. A decamer peptide derived from the Npm sequence sensitized peptide‐pulsed HLA‐A1 positive cells to lysis by the CTL line. The peptide also induced proliferative and cytotoxic T lymphocytes in the PBMC of 4 of 6 CRC patients, which lysed HLA‐A1 positive peptide‐pulsed target cells and CRC cells endogenously expressing Npm. Overexpression of Npm by tumors of various histological types, recognition of the antigen by T cells derived from different CRC patients and association of the antigen with poor prognostic outcome make it a promising target for immunotherapeutic intervention in cancer patients.
Rape and sexual assault in investigative psychology: the contribution of sex offenders' research to offender profiling
Authors: Beauregard Eric 
Published: December 2009

AbstractResearch on sex offenders has mainly guided clinical practice for risk assessment and therapeutic intervention. However, the current scientific knowledge on these offenders and their crimes is, in many aspects, of great importance to criminal investigations. Consequently, there is a need to build bridges between investigative psychology and the research being conducted on sex offenders. Four areas of research on sex offenders that have clear implications to investigative psychology can be identified: (1) the consistency or ‘crime‐switching’ patterns of sex offenders; (2) the recidivism patterns of different types of sex offenders; (3) the police response to specific victim characteristics; and (4) the A → C equation of sexual assaults. This paper argues for a need to establish a dialogue between these two fields of research so that knowledge about sex offenders keeps growing whilst being able to inform policing practices in investigative psychology. Copyright © 2009 John Wiley & Sons, Ltd.
Flow‐independent T2‐prepared inversion recovery black‐blood MR imaging
Authors: Liu Chia‐Ying , Bley Thorsten A. , Wieben Oliver , Brittain Jean H. , Reeder Scott B. 
Published: December 2009

AbstractPurpose:To develop a magnetization preparation method to achieve robust, flow‐independent blood suppression for cardiac and vascular magnetic resonance imaging (MRI).Materials and Methods:T2Prep‐IR sequence consists of a T2 preparation followed by a nonselective adiabatic inversion pulse. T2Prep separates the initial longitudinal magnetization of arterial wall from lumen blood. After the inversion recovery pulse the imaging acquisition is then delayed for a period that allows the blood signal to approach the zero‐crossing point. Compared to the conventional double inversion recovery (DIR) preparation, T2Prep‐IR prepares all the spins regardless of their velocity and direction. T2Prep‐IR was incorporated into the fast spin echo and fast gradient echo acquisition sequences and images in various planes were acquired in the carotid arteries, thoracic aorta, and heart of normal volunteers. Blood suppression and image quality were compared qualitatively between two different preparations.Results:For in‐plane flow carotid images, persistent flow‐related artifacts on the DIR images were removed with T2Prep‐IR. For cardiac applications, T2Prep‐IR provided robust blood suppression regardless of the flow direction and velocity, including the cardiac long‐axis views and the aorta that are often problematic with DIR.Conclusion:T2Prep‐IR may overcome the flow dependence of DIR by providing robust flow‐independent black‐blood images. J. Magn. Reson. Imaging 2010;31:248–254. © 2009 Wiley‐Liss, Inc
Comprehensive MR evaluation of renal disease: Added clinical value of quantified renal perfusion values over single MR angiography
Authors: Attenberger Ulrike I. , Sourbron Steven P. , Schoenberg Stefan O. , Morelli John , Leiner Tim , Schoeppler Gita M. , Samtleben Walter , Birkemeier Krista L. , Glaser Christian , Reiser Maximilian F. , Michaely Henrik J. 
Published: December 2009

AbstractPurpose:To evaluate the diagnostic accuracy of quantified renal perfusion parameters in identifying and differentiating renovascular from renal parenchymal disease.Materials and Methods:In all, 27 patients underwent renal perfusion measurements on a 3.0 T magnetic resonance imaging (MRI) system. Imaging was performed with a saturation recovery TurboFLASH sequence (TR/TE 177/0.93 msec, flip angle 12°, 5 slices/sec). All patients also underwent high‐resolution MR angiography (MRA) (TR/TE 3.1/1.09, flip angle 23°, spatial resolution 0.9 × 0.8 × 0.9 mm3). MR perfusion measurements were analyzed with a two‐compartment model, quantifying the plasma flow (FP)—a characteristic renal first‐pass perfusion parameter. A receiver‐operator characteristic analysis was used to determine the optimal threshold value for distinguishing normal and abnormal plasma flow values. Utilizing this cutoff, sensitivity and specificity of solitary MR perfusion measurements, MRA, and a diagnostic strategy combining the two were evaluated.Results:Quantified MR perfusion values yielded a sensitivity of 100% and a specificity of 85% utilizing the optimal plasma flow threshold value of 150 mL/100 mL/min, whereas single MRA achieved a sensitivity of 51.9% and a specificity of 90%. Combining both methods enabled improved detection of renovascular and renoparenchymal disease with a sensitivity of 96.3% and specificity of 90%.Conclusion:In distinction to MRA, quantified MR perfusion measurements allow for the detection of pure renal parenchymal disorders. The combination of MRA with these perfusion measurements suggests an algorithm by which parenchymal and renovascular diseases may be reliably distinguished and the hemodynamic significance of the latter reliably determined. J. Magn. Reson. Imaging 2010;31:125–133. © 2009 Wiley‐Liss, Inc.
Short echo time 1H MRSI of the human brain at 3T with adiabatic slice‐selective refocusing pulses; reproducibility and variance in a dual center setting
Authors: Wijnen Jannie P. , van Asten Jack J.A. , Klomp Dennis W.J. , Sjobakk Torill E. , Gribbestad Ingrid S. , Scheenen Tom W.J. , Heerschap Arend 
Published: December 2009

AbstractPurpose:To assess the reproducibility of 1H‐MR spectroscopic imaging (MRSI) of the human brain at 3T with volume selection by a double spin echo sequence for localization with adiabatic refocusing pulses (semi‐LASER).Materials and Methods:Twenty volunteers in two different institutions were measured twice with the same pulse sequence at an echo time of 30 msec. Magnetic resonance (MR) spectra were analyzed with LCModel with a simulated basis set including an experimentally acquired macromolecular signal profile. For specific regions in the brain mean metabolite levels, within and between subject variance, and the coefficient of variation (CoV) were calculated (for taurine, glutamate, total N‐acetylaspartate, total creatine, total choline, myo‐inositol + glycine, and glutamate + glutamine).Results:Repeated measurements showed no significant differences with a paired t‐test and a high reproducibility (CoV ranging from 3%–30% throughout the selected volume). Mean metabolite levels and CoV obtained in similar regions in the brain did not differ significantly between two contributing institutions. The major source of differences between different measurements was identified to be the between‐subject variations in the volunteers.Conclusion:We conclude that semi‐LASER 1H‐MRSI at 3T is an adequate method to obtain quantitative and reproducible measures of metabolite levels over large parts of the brain, applicable across multiple centers. J. Magn. Reson. Imaging 2010;31:61–70. © 2009 Wiley‐Liss, Inc.
Imaging cerebral microbleeds using susceptibility weighted imaging: One step toward detecting vascular dementia
Authors: Ayaz Muhammad , Boikov Alexander S. , Haacke E. Mark , Kido Daniel K. , Kirsch Wolff M. 
Published: December 2009

AbstractPurpose:To monitor changes in the number of cerebral microbleeds (CMBs) in a longitudinal study of healthy controls (HC) and mild‐cognitively impaired (MCI) patients using susceptibility weighted imaging (SWI).Materials and Methods:SWI was used to image 28 HC and 75 MCI patients annually at 1.5 Tesla over a 4‐year period. Magnitude and phase data were used to visualize CMBs for the first and last scans of 103 subjects.Results:Preliminary analysis revealed that none of the 28 HC had more than three CMBs. In the 75 MCI patients, five subjects had more than three CMBs in both first and last scans, while one subject had more than three bleeds only in the last scan. In five of these six MCI patients, the number of CMBs increased over time and all six went on to develop progressive cognitive impairment (PCI). Of the 130 total CMBs seen in the last scans of the six MCI cases, most were less than 4 mm in diameter.Conclusion:SWI can reveal small CMBs on the order of 1 mm in diameter and this technique can be used to follow their development longitudinally. Monitoring CMBs may be a means by which to evaluate patients for the presence of microvascular disease that leads to PCI. J. Magn. Reson. Imaging 2010;31:142–148. © 2009 Wiley‐Liss, Inc.
Direct comparison of sensitivity encoding (SENSE) accelerated and conventional 3D contrast enhanced magnetic resonance angiography (CE‐MRA) of renal arteries: Effect of increasing spatial resolution
Authors: Muthupillai R. , Douglas E. , Huber S. , Lambert B. , Pereyra M. , Wilson G.J. , Flamm S.D. 
Published: December 2009

AbstractPurpose:To assess the effect of attaining higher spatial resolution in contrast‐enhanced magnetic resonance angiography (MRA) of renal arteries using parallel imaging, sensitivity encoding (SENSE), by comparing the SENSE contrast‐enhanced (CE) MRA against a conventional CE‐MRA protocol with identical scan times, injection protocol, and other acquisition parameters.Materials and Methods:Numerical simulations and a direct comparison of SENSE‐accelerated versus conventional acquisitions were performed. A total of 41 patients (18 male) were imaged using both protocols for a direct comparison. Both protocols used fluoroscopic triggering, centric encoding, breath‐holding, equivalent injection protocol, and lasted ≈30 seconds.Results:Simulated point‐spread functions were narrower for the SENSE protocol compared to the conventional protocol. In the patient study, although the SENSE protocol produced images with lower signal‐to‐noise ratio (SNR), image quality was better for all segments of the renal arteries. In addition, ringing of kidney parenchyma and renal artery blurring were significantly reduced in the SENSE protocol. Finally, reader confidence improved with the SENSE protocol.Conclusion:Despite a reduction in SNR, the higher‐resolution SENSE CE‐MRA provided improved image quality, reduced artifacts, and increased reader confidence compared to the conventional protocol. J. Magn. Reson. Imaging 2010;31:149–159. © 2009 Wiley‐Liss, Inc.