bluuebunny/crossref_metadata_2025_split huggingface dataset:
Region 8q24 is a susceptibility locus for nonsyndromic oral clefting in Brazil
Authors: Brito Luciano Abreu , Paranaiba Lívia Máris Ribeiro , Bassi Camila Fernandes Silva , Masotti Cibele , Malcher Carolina , Schlesinger David , Rocha Katia Maria , Cruz Lucas Alvizi , Bárbara Lígia Kobayashi , Alonso Nivaldo , Franco Diogo , Bagordakis Elizabete , Martelli Hercílio , Meyer Diogo , Coletta Ricardo D. , Passos‐Bueno Maria Rita 
Published: April 2012

AbstractBACKGROUNDNonsyndromic cleft lip with or without cleft palate is a relatively common craniofacial defect with multifactorial inheritance. The association of the rs987525 single nucleotide variant, located in a gene desert at 8q24.21 region, has been consistently replicated in European populations. We performed a structured association approach combined with transcriptional analysis of the MYC gene to dissect the role of rs987525 in oral clefting susceptibility in the ethnically admixed Brazilian population.METHODSWe performed the association study conditioned on the individual ancestry proportions in a sample of 563 patients and 336 controls, and in an independent sample of 221 patients and 261 controls. The correlation between rs987525 genotypes and MYC transcriptional levels in orbicularis oris muscle mesenchymal stem cells was also investigated in 42 patients and 4 controls.RESULTSWe found a significant association in the larger sample (p = 0.0016; OR = 1.80 [95% confidence interval {CI}, 1.21–2.69], for heterozygous genotype, and 2.71 [95% CI, 1.47–4.96] for homozygous genotype). We did not find a significant correlation between rs987525 genotypes and MYC transcriptional levels (p = 0.14; r = −0.22, Spearman Correlation).CONCLUSIONSWe present a positive association of rs987525 in the Brazilian population for the first time, and it is likely that the European contribution to our population is driving this association. We also cannot discard a role of rs987515 in MYC regulation, because this locus behaves as an expression quantitative locus of MYC in another tissue. Birth Defects Research (Part A), 2012. © 2012 Wiley Periodicals, Inc.
A new fluorescent chemosensor for Hg2+ in aqueous solution
Authors: Quang Duong Tuan , Hop Nguyen Van , Luyen Nguyen Dinh , Thu Ha Phuong , Oanh Doan Yen , Hien Nguyen Khoa , Hieu Nguyen Van , Lee Min Hee , Kim Jong Seung 
Published: April 2012

ABSTRACTWe prepared an aminothiourea‐derived Schiff base (DA) as a fluorescent chemosensor for Hg2+ ions. Addition of 1 equiv of Hg2+ ions to the aqueous solution of DA gave rise to an obvious fluorescence enhancement and the subsequent addition of more Hg2+ induced gradual fluorescence quenching. Other competing ions, including Pb2+, Cd2+, Cr3+, Zn2+, Fe2+, Co3+, Ni2+, Ca2+, Mg2+, K+ and Na+, did not induce any distinct fluorescence changes, indicating that DA can selectively detect Hg2+ ions in aqueous solution. Copyright © 2012 John Wiley & Sons, Ltd.
Interactions between m‐phenylenediamine and bovine serum albumin measured by spectroscopy
Authors: Chen Jian‐qiu , Hu Zhi‐jun , Wang Nan‐xi , Ji Rong 
Published: April 2012

ABSTRACTThis study explored interactions between m‐phenylenediamine (MPD) and bovine serum albumin (BSA) by spectrophotometry. The Stern‐Volmer equation and UV‐vis spectra examination at different temperatures and pH were used to explore different quenching mechanisms. Under simulated physiological conditions, the binding distance between MPD and BSA was 5.18 nm with a ratio of 1:1. The quenching effect of MPD on BSA intrinsic fluorescence depended strongly on pH, and maximum quenching was observed at alkaline pH. Moreover, the thermodynamic parameters of the MPD‐BSA system showed that the predominant acting force between MPD and BSA was a hydrophobic force. The impact of MPD on the conformation of BSA and the effects of co‐ions on binding interactions were also examined. Copyright © 2012 John Wiley & Sons, Ltd.
Mapping Corporate Responsibility and Sustainable Supply Chains: an Exploratory Perspective
Authors: Carbone Valentina , Moatti Valerie , Vinzi Vincenzo Esposito 
Published: April 2012

ABSTRACTCorporate responsibility (CR) in general, and sustainable supply chain management in particular, have been a growing concern for companies and researchers over the past decade. However, in scholarly work, sustainability has often been dealt with in a generic fashion or from an anecdotal point of view. Further, research works examining CR on the one hand and sustainable supply chains on the other have been conducted separately. We undertake the multiple factor analysis of a CR rating database (Innovest) which reports longitudinal scores for both the social and environmental performance of 1198 companies in different countries and distinct industries, to demonstrate a strong relationship between CR and a sustainable supply chain. Our findings from exploratory analysis also illustrate the role of country of origin and industry in shaping CR behaviour, highlighting isomorphic as well as allomorphic trends for CR trough time. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment.
Comparisons of clinical outcomes for thrombectomy devices with different mechanisms in hemodialysis arteriovenous fistulas
Authors: Yang Chih‐Chung , Yang Chung‐Wei , Wen Szu‐Chi , Wu Chih‐Cheng 
Published: April 2012

AbstractObjectivesTo compare clinical outcomes between mechanical thrombectomy devices with hydrodynamic mechanism and rotational mechanism.BackgroundA number of advantages and concerns have been raised for different mechanical devices but the comparisons of clinical outcomes are absent, especially for the treatment of autogenous hemodialysis arteriovenous (AV) fistulas.MethodsThe authors retrospectively reviewed 275 percutaneous thrombectomy procedures in AV fistulas. Procedures included were thrombectomy using devices with hydrodynamic mechanism, the AngioJet rheolytic catheter (AngioJet) (n = 134) or devices with rotational mechanism, the Arrow‐Trerotola percutaneous thrombectomy device (PTD) (n = 141). Measured outcomes included clinical success, complications and patency rates.ResultsClinical success was achieved in 76% (102 of 134) of the AngioJet procedures and in 91% (128 of 141) of the PTD procedures (P = 0.002). The procedure time for the PTD method was significantly shorter than that of the AngioJet method (52 vs. 88 min, P < 0.001). Complications occurred in 14% of the AngioJet procedures and 11% of the PTD procedures. The post‐intervention primary patency rates at 6 months were 45% for the AngioJet group and 43% for the PTD group (P = 0.70). The postintervention secondary patency rates at 1 year were 74% for the AngioJet group and 87% for the PTD group (P = 0.01).ConclusionsFor the salvage of thrombosed AV fistulas, a device using rotational mechanism is more time‐saving with a higher immediate success rate and secondary patency rate. However, the long‐term patency results were not different. © 2012 Wiley Periodicals, Inc.
Significant reduction in systolic blood pressure following renal artery stenting in patients with uncontrolled hypertension: Results from the HERCULES trial
Authors: Jaff Michael R. , Bates Mark , Sullivan Timothy , Popma Jeffrey , Gao Xingyu , Zaugg Margo , Verta Patrick ,   on behalf of the HERCULES Investigators
Published: April 2012

AbstractBackground: Atherosclerotic renal artery stenosis (ARAS) causes hypertension (HTN) and threatens renal function (RF). The HERCULES Trial is a prospective, multicenter trial of renal stenting in patients with uncontrolled HTN and ARAS evaluating the safety and effectiveness of the RX Herculink Elite Renal Stent System (Abbott Vascular, Santa Clara, CA). Methods: A total of 202 patients (241 total lesions; 78 bilateral lesions) were enrolled between August 2007 and October 2009. The primary endpoint was 9‐month binary restenosis determined by duplex ultrasound and/or angiography. Secondary endpoints included changes in blood pressure, antihypertensive medications, and RF between baseline and 9 months. Brain natriuretic peptide (BNP) was measured at baseline, 24 hr and 30 days postprocedure. Results: Mean systolic blood pressure (SBP) at baseline was 162 mm Hg. Nearly 70% of patients were receiving three or more antihypertensive medications (mean 3.4 medications per patient). Baseline serum creatinine was 1.2 ± 0.4 and 61.5% of subjects had estimated glomerular filtration <60. The restenosis rate was 10.5% at 9 months. The study device, procedure, and clinical success rates were 96.0, 99.2, and 98.0%, respectively. Freedom from major adverse events was 94.8%. At 9 months, the mean SBP significantly decreased (mean 145, paired t test P < 0.0001) after stenting with no change in medications. There was no correlation between SBP reduction and baseline BNP or BNP reduction. Conclusions: HERCULES demonstrates clinically and statistically significant SBP reduction in patients with uncontrolled HTN, low in‐stent restenosis, and complication rates. This study highlights that when appropriate patients are selected for renal artery stenting, impressive reductions in blood pressure may be anticipated. Although the magnitude of absolute reduction in SBP was related to baseline SBP, elevated baseline BNP levels were not predictive of reduction in SBP. Further studies for predictors of clinical response following percutaneous renal revascularization are needed. © 2012 Wiley Periodicals, Inc.
Long‐term impact of balloon postdilatation on neointimal formation: An experimental comparative study between second‐generation self‐expanding versus balloon‐expandable stent technologies
Authors: Aboodi Michael S. , Milewski Krzysztof , Tellez Armando , Cheng Yanping , Yi Geng‐Hua , Kaluza Greg L. , Granada Juan F. 
Published: April 2012

BackgroundSelf‐expanding stents (SES) are reemerging as therapeutic alternatives to treat coronary artery disease. It has been proposed that SES can improve clinical outcomes by inducing less injury at implantation and achieving better vessel wall apposition. To date, little data exists comparing the vascular response to both methods of deployment in a controlled experimental setting.ObjectiveTo quantify differences in vascular injury and healing between second‐generation SES and balloon‐expandable stents (BES) and the effects of balloon postdilatation in a porcine coronary model.MethodsSeventy‐five bare SES (AXXESS or vProtect) and 42 BES (Vision) were implanted in porcine coronaries. A subset of these received balloon postdilatation (SES + D = 22, BES + D = 20). Follow‐up was scheduled at 30 (BES = 10, BES + D = 6, SES = 19, SES + D = 8), 90 (BES = 6, BES + D = 8, SES = 19, SES + D = 8), and 180 days (BES = 6, BES + D = 6, SES = 15, SES + D = 6).ResultsIn vivo imaging and histological analysis showed that neointimal formation peaks early (30 days) in BES. Conversely, for SES, the peak occurred later (90 days). However, the neointimal formation achieved in either group equalized at 180 days. For SES, postdilatation shortened the peak of neointimal formation to 30 days. Conversely, for BES, postdilatation delayed the peak of neointimal formation to 90 days. At 30 days, histology showed that SES had significantly less injury. However, at 90 days, injury scores tended to be higher for SES. By 180 days, injury scores were comparable between both groups.ConclusionsThe mechanism of stent expansion influences the degree of vascular injury and healing. The synergistic use of balloon postdilatation changes the dynamics of healing and may impact the potential beneficial effects inherent to SES technologies. © 2012 Wiley Periodicals, Inc.
Effect of Ultrasonic Irradiation on the Crystallization of Sodium Acetate Trihydrate Utilized as Heat Storage Material
Authors: Seo K. , Suzuki S. , Kinoshita T. , Hirasawa I. 
Published: April 2012

AbstractThe effect of ultrasonic irradiation on nucleation phenomena in the heat storage material CH3COONa · 3H2O was investigated by measuring the nucleation probability and induction time. The experimental results show that the concentration of CH3COONa should be operated in the optimum range for CH3COONa · 3H2O‐selective crystallization. Furthermore, the nucleation probability increased and the induction time decreased with increasing energy input. Consequently, the optimum conditions of ultrasonic irradiation of CH3COONa · 3H2O are 53 wt‐% CH3COONa, 3.0 W of energy input, and ΔT = 10–68 K.
Enantioselective Multicomponent Synthesis of Fused 6–5 Bicyclic 2‐Butenolides by a Cascade Heterobicyclisation Process
Authors: Suero Marcos G. , De la Campa Raquel , Torre‐Fernández Laura , García‐Granda Santiago , Flórez Josefa 
Published: April 2012

AbstractThe successive coupling of an alkoxy(aryl/heteroaryl)carbene complex of chromium with either a ketone or an imide lithium enolate and then a 3‐substituted (H, TMS, PhCH2, PhCH2CH2, Me) propargylic organomagnesium reagent has afforded novel hydroxy‐substituted bicyclic [4.3.0]‐γ‐alkylidene‐2‐butenolides with three modular points that has allowed the efficient introduction of molecular complexity, including a homopropargylic alcohol core. The selective formation of these five‐ or six‐component heterobicyclisation products is the result of the regioselective integration of the Grignard reagent as a propargyl fragment followed by a cascade CO/alkyne/CO insertion, ketene trapping and elimination sequence. By using lithium enolates of chiral N‐acetyl‐2‐oxazolidinones and the corresponding propargylic organocerium reagents, both enantiomers of these bicyclic heterocycles were efficiently prepared with very high enantiomeric purity. Architecturally, these fused bicyclic butenolides are characterised by a highly unsaturated and oxygenated core and they exhibit strong blue fluorescence in solution.
From Low to Very High Birefringence in Bis(2‐pyridylimino)isoindolines: Synthesis and Structure–Property Analysis
Authors: Wong Edwin W. Y. , Ovens Jeffrey S. , Leznoff Daniel B. 
Published: April 2012

AbstractA series of new substituted 1,3‐bis(2‐pyridylimino)isoindolines—1,3‐bis(2‐pyridylimino)‐5,6‐bis(2,6‐diisopropylphenoxy)isoindoline (2 b), 1,3‐bis(2‐pyridylimino)‐5,6‐bis(4‐tert‐butylphenyl)isoindoline (2 c), and 1,3‐bis(2‐pyridylimino)‐5‐tert‐butylisoindoline (2 d)—were synthesized and structurally characterized by single‐crystal X‐ray diffraction. The birefringence (Δn) of the crystals of unsubstituted 1,3‐bis(2‐pyridylimino)isoindoline (2 a), 2 b, 2 c, and 2 d were measured and found to vary greatly, with Δn values of 0.0654(3), 0.0629(17), 0.588(10), 0.701(12), respectively. A structure–property relationship for the birefringence values of 2 a–2 d was outlined and indicated that the anisotropy of the polarizability of the molecules plays a crucial role in the birefringence of the crystals. The greatest birefringence values are achieved when the molecules are oriented in a face‐to‐face configuration intermolecularly, and along the crystallographic face being measured.
Catalytic Epoxidations with Peroxides: Molybdenum Trioxide Species as the Origin of Allylic Byproducts
Authors: Neuenschwander Ulrich , Meier Emanuel , Hermans Ive 
Published: April 2012

AbstractMolybdenum(VI)peroxide species, formed in the reaction of MoVI complexes with peroxides, are able to epoxidize >CC< double bonds heterolytically. In this study, theoretical and experimental evidence is provided for a kinetically competing reaction reaction of such molybdenum(VI)peroxide species with additional peroxide reagent, leading to molybdenum(VI)trioxide species, which easily decompose into radicals. Under epoxidation conditions, those radicals will reduce the selectivity, due to the formation of allylic byproducts. The involved reaction pathways are characterized by DFT calculations, providing kinetic parameters that are in good agreement with the experimental observations.
Hedgehog regulates angiogenesis of intersegmental vessels through the VEGF signaling pathway
Authors: Moran Carlos M. , Myers Candace T. , Lewis Cristy M. , Krieg Paul A. 
Published: April 2012

AbstractBackground: The cellular mechanisms regulating branching and growth of the intersegmental vessels (ISVs) are not well understood. We have carried out studies demonstrating that Hedgehog (Hh) signaling is a major regulator of intersomitic vessel growth. Results: Inhibition of Hh activity by cyclopamine completely blocks formation of intersomitic vessels in the avian embryo. Examination of gene expression patterns in Hh‐deficient embryos shows that components of the VEGF and Notch signaling pathways are down‐regulated. However, we find no evidence that Notch signaling plays a significant role in regulation of intersomitic vessel growth. Indeed, it appears that Hh modulation of Vascular Endothelial Growth Factor, VEGF, is the primary regulator of growth of intersomitic vessels in the avian embryo. Conclusions: Inhibition of the VEGF pathway results in absence of ISVs, whereas stimulation of VEGF expression leads to precocious branching of ISVs. These results demonstrate that Hh is an essential modulator of VEGF expression during developmental angiogenesis. Developmental Dynamics 241:1034–1042, 2012. © 2012 Wiley Periodicals, Inc.
Epigenetic regulation of cardiac development and function by polycomb group and trithorax group proteins
Authors: Wang Q. Tian 
Published: April 2012

AbstractHeart disease is a leading cause of death and disability in developed countries. Heart disease includes a broad range of diseases that affect the development and/or function of the cardiovascular system. Some of these diseases, such as congenital heart defects, are present at birth. Others develop over time and may be influenced by both genetic and environmental factors. Many of the known heart diseases are associated with abnormal expression of genes. Understanding the factors and mechanisms that regulate gene expression in the heart is essential for the detection, treatment, and prevention of heart diseases. Polycomb Group (PcG) and Trithorax Group (TrxG) proteins are special families of chromatin factors that regulate developmental gene expression in many tissues and organs. Accumulating evidence suggests that these proteins are important regulators of development and function of the heart as well. A better understanding of their roles and functional mechanisms will translate into new opportunities for combating heart disease. Developmental Dynamics 241:1021–1033, 2012. © 2012 Wiley Periodicals, Inc.
Climate Change? No, Wise Resource Use is the Issue: Social Representations of Energy, Climate Change and the Future
Authors: Fischer Anke , Peters Vera , Neebe Mirjam , Vávra Jan , Kriel Antoinette , Lapka Miloslav , Megyesi Boldizsár 
Published: April 2012

ABSTRACTRecent social scientific research has often dealt with public perceptions of climate change as an isolated topic. In contrast, we explored through qualitative interviews (n = 202) across five European countries how members of the public conceptualized climate change within a wider context of energy and the future, drawing on social representation theory.Our analysis suggests that, rather than separating between environmental issues, participants interpreted both climate change and energy consumption in a context of unsustainable resource use. Views on climate change were often very sceptical, but the unsustainability of contemporary resource use was almost unanimously of great concern. However, despite their strong plea for political change towards sustainability, many interviewees found it challenging to translate their views into behaviour. We argue that obstacles for behavioural change might lie in the tensions between cognitive, normative and affective aspects of people's representations, and conclude with recommendations for European climate change mitigation policies. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment
Label‐Free Amperometric Detection of Albumin with an Oil/Water‐type Flow Cell for Urine Protein Analysis
Authors: Matsui Risa , Sakaki Toru , Osakai Toshiyuki 
Published: April 2012

AbstractThe 1,2‐dichloroethane (DCE)/water interface, with an anionic surfactant, dinonylnaphthalenesulfonate (DNNS−), being present in DCE, was utilized for label‐free detection of albumin. An oil/water‐type flow cell was prepared using a porous PTFE tube and dipping the tube in the DCE solution containing DNNS−. This flow cell provided a well‐defined current response linear to the albumin concentration up to 10 µM with a detection limit of 1.2 µM. The current response is due to the interfacial adsorption of albumin molecules depending on the Galvani potential difference. Possible interference from creatinine in the urine could be avoided by a conventional dialysis treatment.
Genotype analysis of tumor‐initiating cells expressing CD133 in neuroblastoma
Authors: Cournoyer Sonia , Nyalendo Carine , Addioui Anissa , Belounis Assila , Beaunoyer Mona , Aumont Anne , Teira Pierre , Duval Michel , Fernandes Karl , Fetni Raouf , Haddad Elie , Sartelet Hervé 
Published: April 2012

AbstractNeuroblastoma (NB) is the most common and lethal extracranial solid tumor of childhood. Despite aggressive therapy, more than half of the children with advanced NB will die of uncontrolled metastatic disease. After chemotherapy, tumor‐initiating cells (TICs) could persist, cause relapses and metastasis. The aim of this study is to demonstrate the tumor‐initiating properties of CD133high NB cells and to identify new specific genetic abnormalities. Isolation of the CD133high cell population from NB cell lines was followed by neurosphere formation, soft agar assays, and orthotopic injections in NOD/SCID/IL2Rγc‐null mice. A differential genotyping analysis was performed with Affymetrix SNP 6.0 arrays on CD133low and CD133high populations and the frequency of the abnormalities of 36 NB tumors was determined. Our results show that CD133high NB cells possess tumor‐initiating properties, as CD133high cells formed significantly more neurospheres and produced significantly more colonies in soft agar than CD133low. Injection of 500 CD133high cells was sufficient to generate primary tumors and frequent metastases in mice. Differential genotyping analysis demonstrated two common regions with gains (16p13.3 and 19p13.3) including the gene EFNA2 in the CD133high population, and two with loss of heterozygosity (16q12.1 and 21q21.3) in the CD133low population. The gain of EFNA2 correlated with increased expression of the corresponding protein. These abnormalities were found in NB samples and some were significantly correlated with CD133 expression. Our results show that CD133high NB cells have TICs properties and present different genotyping characteristics compared to CD133low cells. Our findings reveal insights into new therapeutic targets in NB TICs. © 2012 Wiley Periodicals, Inc.