bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anterior spinal artery syndrome: a rare complication of head and neck surgery
Authors: Gadepalli C , Cardozo A , Loughran S 
Published: March 2010

AbstractObjective:We report a case of anterior spinal artery syndrome, an extremely rare complication, following head and neck surgery.Method:A case report and literature review concerning anterior spinal artery syndrome is presented.Results:A 64-year-old man developed an anterior spinal artery infarction following total laryngectomy and bilateral neck dissections for post-radiotherapy glottic carcinoma. Anterior spinal artery infarction is a rare syndrome. It typically presents with weakness, loss of pain and temperature sensation below the level of the injury, with relative sparing of position and vibratory sensation. Recovery is variable.Conclusion:To the best of our knowledge, this is the first case report in the English language literature of anterior spinal artery syndrome following a head and neck procedure. This case report highlights a rare complication, and also the susceptibility of head and neck surgery patients to different complications. In head and neck cancer patients suffering anterior spinal artery syndrome following primary surgical treatment, we recommend that the management of this complication should be as aggressive as that of the primary cancer.
Adjusting Chinese income inequality for environmental equity
Authors: BRAJER VICTOR , MEAD ROBERT W. , XIAO FENG 
Published: February 2010

ABSTRACTGiven the extensive discussion of China's air pollution and urban income inequality problems, relatively little attention has been paid to any relationship which may exist between the two. This paper seeks to do so, by exploring the existence of environmental equity in China. Specifically, we first calculate traditional measures of urban income inequality (the Gini coefficient and Theil's T and L indices) for the years 1995 and 2004. Then, we estimate ‘pollution-adjusted’ incomes using China-based health functions and economic valuation studies, and recalculate the income (or now welfare) inequality measures. The results indicate that as pollution cleanup efforts gain traction over time, the regressive consequences of pollution may diminish. We thus conclude with an encouraging note for developing countries: improving welfare distribution can coexist with an improving environment.
Variability of the solar spectral irradiance and energetic particles
Authors: Silva-Válio Adriana 
Published: February 2010

AbstractThe total spectral irradiance of the Sun is seen to vary on many time scales. Three timescales are more prominent: (1) the longest one of about 11 years; (2) an intermediate timescale of the order of a few weeks; and (3) the shortest variation from hours to seconds. Every 11 years, the total solar irradiance periodically shows intervals of great activity and periods of almost no activity. The peak to peak variability, however, is less than 0.1%. This periodic variation of 11 years is called the solar cycle, which main tracer are sunspots. During times of maximum activity, there are many sunspots on the surface of the Sun, whereas during minimum there may be none. Sunspots are dark, and therefore cool, regions of enhanced magnetic fields of about a few hundred Gauss, that usually appear in groups on the solar photosphere. Basically, the solar cycle is regulated by the magnetic dynamo acting below the solar surface. Right now, the Sun is going through a time of minimum activity. The sunspot lifetime is of the order of one to two weeks, and are thus responsible for the intermediate variability timescale. The magnetic loops seen in ultraviolet and X-ray images have their footpoints anchored on sunspots. The most energetic phenomena of solar activity are flares and coronal mass ejections. Flares are large explosions that occur on the solar atmosphere and may last from a few seconds to hours. A solar flare is caused by a sudden, and yet unpredicted, energy release high above the magnetic loops. This magnetic energy is then used into particle acceleration and heating of the surrounding atmosphere. Both the energetic particles and the hot gas produce emission throughout the whole electromagnetic spectrum, from the very energetic gamma-rays all the way to long radio waves. From the observation of the emission produced during flares it is possible to infer the energetic particles spectra and thus have a clue on the acceleration mechanism that produced these particles. The recent findings of flare observations at gamma-rays by the RHESSI satellite and at high radio frequencies by the Solar Submillimeter Telescope are presented and discussed.
The asymmetric light curves of the GSC 2764 1417 (And), GSC 3355 0394 (Per) and GSC 2537 0520 (Psc)
Authors: Samec Ronald G. , Figg Evan R. , Melton Reid , Labadorf Christa M. , Miller Jess , McKenzie Robert , Faulkner Danny R. , Van Hamme Walter 
Published: February 2010

AbstractSolar-type variability is enhanced in short period close binaries with increased dynamo driven activity. This activity is studied in our analysis of recent light curves taken of the newly discovered eclipsing binaries GSC 2764 1417 (And), GSC 3355 0394 (Per) and GSC 2537 0775 (CVn).
The formation of an equatorial coronal hole
Authors: Yang Liheng , Jiang Yunchun , Zhang Jun 
Published: February 2010

AbstractThe formation of an equatorial coronal hole (CH) from 2006 January 9 to 12 was simultaneously observed byGOES-12/SXI,SOHO/EIT andSOHO/MDI instruments. The varieties of soft X-ray and EUV brightness, coronal temperature, and total magnetic flux in the CH were examined and compared with that of a quiet-sun (QS) region nearby. The following results are obtained. (1) A preexisting dark lane appeared on the location of the followed CH and was reinforced by three enhanced networks. (2) The CH gradually formed in about 81 hours and was predominated by positive magnetic flux. (3) During the formation, the soft X-ray and EUV brightness, coronal temperature, and total magnetic flux obviously decreased in the CH, but were almost no change in the QS region. The decrease of the total magnetic flux may be the result of magnetic reconnection between the open and closed magnetic lines, probably indicating the physical mechanism for the birth of the CH.
Manifestations of dark energy in the dynamics of the Solar system
Authors: Křížek Michal , Brandts Jan 
Published: February 2010

AbstractThe expansion speed of the Universe is increasing (Glanz 1998). This acceleration is attributed to dark energy which acts almost uniformly everywhere (including the Solar system) and thus essentially influences the Hubble constant. Its current value on a distance of 1 AU is H0 = 10 m/(yr AU). This is quite a large number and thus, the impact of dark energy should be detectable in the Solar system. We will illustrate it by several examples. Dark energy may partially be caused by gravitational aberration of the Sun, planets and other bodies.
UV habitability and dM stars: an approach for evaluation of biological survival
Authors: Abrevaya Ximena C. , Cortón Eduardo , Mauas Pablo J. D. 
Published: February 2010

AbstractDwarf M stars comprise about 75 percent of all stars in the galaxy. For several years planets orbiting M stars have been discarded as suitable places for development of life. This paradigm now has changed and terrestrial-type planets within liquid-water habitable zones (LW-HZ) around M stars are reconsidered as possible hosts for life as we know it. Nevertheless, large amount of UV radiation is emitted during flares by this stars, and it is uncertain how these events could affect biological systems. In particular UV-C λ < 290nm) exhibits the most damaging effects for living organisms. To analyze the hypothesis that UV could set a limit for the development of extraterrestrial life, we studied the effect of UV-C treatment on halophile archaea cultures. Halophile archaea are extremophile organisms, they are exposed to intense solar UV radiation in their natural environment so they are generally regarded as relatively UV tolerant. Halophiles inhabits in hipersaline environments as salt lakes but also have been found in ancient salt deposits as halites and evaporites on Earth. Since evaporites have been detected in Martian meteorites, these organisms are proposed as plausible inhabitants of Mars-like planets. Our preliminary results show that even after UV damage, the surviving cells were able to resume growth with nearly normal kinetics.
Exoplanet environments to harbour extremophile life
Authors: Janot-Pacheco Eduardo , Lage Claudia A. S. , Lima Ivan G. P. 
Published: February 2010

AbstractIn this contribution, we estimate the temperature at the surface of known exoplanets and of their putative satellites for two albedo extreme cases (Venus and Mars) and present a selection of extremophiles living on Earth that can live under those conditions. We examine also the possibility of survival of microorganisms in planetary systems of variable stars.
Does the lunar regolith contain secrets of the Solar System? Using the Moon as a cosmic witness plate
Authors: McKay David S. , Riofrio Louise , Cooper Bonnie L. 
Published: February 2010

AbstractThe lunar regolith (soil) has recorded a history of the early Moon, the Earth, and the entire solar system. A major goal of the developing lunar exploration program should be to find and play back existing fragments of that tape. By playing back the lunar tape, we can uncover a record of planetary bombardment, as well as solar and stellar variability. The Moon can tell us much about our place in the solar system and in the Universe. The lunar regolith has likely recorded the original meteoritic bombardment of Earth and Moon, a violent cataclysm that may have peaked around 4 GY, and the less intense bombardment occurring since that time. Decrease in bombardment allowed life to develop on Earth. This impact history is preserved as megaregolith layers, ejecta layers, impact melt rocks, and ancient impact breccias. The impact history for the Earth and Moon possibly had profound effects on the origin and development of life. Life may have arrived via meteorite transport from a more quiet body, such as Mars. The solar system may have experienced bursts of severe radiation from the Sun, other stars or from unknown sources. The lunar regolith has also recorded a radiation history in the form of implanted and trapped solar wind and solar flare materials and radiation damage. The Moon can be considered as a giant tape recorder containing the history of the solar system. Lunar soil generated by small impacts will be found sandwiched between layers of basalt or pyroclastic deposits. This filling constitutes a buried time capsule that is likely to contain well-preserved ancient regolith. Study of such samples will show us how the solar system has evolved and changed over time. The lunar recording can provide detailed snapshots of specific portions of solar and stellar variability.
Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124
Authors: Auld Douglas S. , Lovell Scott , Thorne Natasha , Lea Wendy A. , Maloney David J. , Shen Min , Rai Ganesha , Battaile Kevin P. , Thomas Craig J. , Simeonov Anton , Hanzlik Robert P. , Inglese James 
Published: March 2010

Firefly luciferase (FLuc), an ATP-dependent bioluminescent reporter enzyme, is broadly used in chemical biology and drug discovery assays. PTC124 (Ataluren; (3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid) discovered in an FLuc-based assay targeting nonsense codon suppression, is an unusually potent FLuc-inhibitor. Paradoxically, PTC124 and related analogs increase cellular FLuc activity levels by posttranslational stabilization. In this study, we show that FLuc inhibition and stabilization is the result of an inhibitory product formed during the FLuc-catalyzed reaction between its natural substrate, ATP, and PTC124. A 2.0 Å cocrystal structure revealed the inhibitor to be the acyl-AMP mixed-anhydride adduct PTC124-AMP, which was subsequently synthesized and shown to be a high-affinity multisubstrate adduct inhibitor (MAI;
K

D

= 120 pM) of FLuc. Biochemical assays, liquid chromatography/mass spectrometry, and near-attack conformer modeling demonstrate that formation of this novel MAI is absolutely dependent upon the precise positioning and reactivity of a key
meta
-carboxylate of PTC124 within the FLuc active site. We also demonstrate that the inhibitory activity of PTC124-AMP is relieved by free coenzyme A, a component present at high concentrations in luciferase detection reagents used for cell-based assays. This explains why PTC124 can appear to increase, instead of inhibit, FLuc activity in cell-based reporter gene assays. To our knowledge, this is an unusual example in which the “off-target” effect of a small molecule is mediated by an MAI mechanism.
Engineering cis-telomerase RNAs that add telomeric repeats to themselves
Authors: Qiao Feng , Goodrich Karen J. , Cech Thomas R. 
Published: March 2010

Telomerase is a ribonucleoprotein complex consisting of a protein reverse transcriptase (TERT) and an RNA subunit (TR). Telomerase normally adds telomeric DNA repeats to chromosome ends. Here, we engineer human and
Tetrahymena
cis-telomerase RNAs, each having a DNA primer covalently linked to its 3′ end. We find that cis-telomerase synthesizes DNA with increased repeat addition processivity (RAP) but does not completely rescue the RAP defect of the L14A mutant of
Tetrahymena
TERT. This supports the conclusion that L14 has a function beyond binding the DNA primer and preventing dissociation during multiple rounds of repeat addition. By comparing cis-telomerases with various linker lengths, we find that a 5 nt linker gives near-optimal activity, indicating that the distance between the 3′ end of the telomerase RNA pseudoknot region and the 5′ end of the DNA primer is ∼33
Å
. Even a 2 nt linker (∼14
Å
) gives some activity, indicating a high degree of conformational flexibility in this ribonucleoprotein complex. More generally, the cis system will allow structure-function relationships of each RNA molecule to be read directly through the reaction that it performs on itself.
An unlocking/relocking barrier in conformational fluctuations of villin headpiece subdomain
Authors: Reiner Andreas , Henklein Peter , Kiefhaber Thomas 
Published: March 2010

A reversible structural unlocking reaction, in which the close-packed van der Waals interactions break cooperatively, has been found for the villin headpiece subdomain (HP35) using triplet-triplet-energy transfer to monitor conformational fluctuations from equilibrium. Unlocking is associated with an unfavorable enthalpy change (Δ
H
0
= 35 ± 4 kJ/mol) which is nearly compensated in free energy by the entropy change (Δ
S
0
= 112 ± 20 J·mol
-1
·K
-1
). The unlocking reaction has a time constant of about 1 μs at 5 °C and is enthalpy-limited with an activation energy of 32 ± 1 kJ/mol and a large Arrhenius preexponential factor of
A
= 7.5 × 10
11
s
-1
. In the unlocked state a fast local conformational fluctuation with a time constant of 170 ns and a low activation barrier of 17 ± 1 kJ/mol leads to unfolding of the C-terminal helix and to its undocking from the core. On a much slower time scale, global unfolding occurs from the unlocked state. These results suggest that native protein structures are locked into conformations with low amplitude motions. Large scale motions and global unfolding require an initial structural unlocking step leading to a state with properties of a dry molten globule. The experiments additionally yielded information on the dynamics of loop formation between different positions in unfolded HP35. Comparison of the results with dynamics in unstructured model peptides indicates slightly decelerated kinetics of local loop formation in the C-terminal region which points at residual, nonrandom structure. Dynamics of long-range loop formation, in contrast, are not influenced by residual structure, which argues against unfolded state properties as molecular origin for ultrafast folding of HP35.
A zinc-binding site by negative selection induces metallodrug susceptibility in an essential chaperonin
Authors: Cun Shujian , Sun Hongzhe 
Published: March 2010

GroES is an indispensable chaperonin virtually found throughout all life forms. Consequently, mutations of this protein must be critically scrutinized by natural selection. Nevertheless, the homolog from a potentially virulent gastric pathogen,
Helicobacter pylori
, strikingly features a histidine/cysteine-rich C terminus that shares no significant homology with other family members. Additionally, three more (H45, C51, and C53) are uniquely present in its apical domain. The statistical analyses show that these residues may have originated from negative selection, presumably driven by either dependent or independent amino acid mutations. In the absence of the C-terminal metal-binding domain, the mutant protein still exhibits a substantial capacity for zinc binding in vivo. The biochemical properties of site-directed mutants indicate that H45, C51, and C53 make up an oxidation-sensitive zinc-binding site that may donate the bound metal to a zinc acceptor. Of interest, bismuth antiulcer drugs strongly bind at this site (
K
d
of approximately 7 × 10
-26
M), replacing the bound zinc and consequently inducing the disruption of the quaternary structure. Because biological features by negative selection are usually inert to change during evolution, this study sheds light on a promising field whereby medicines can be designed or improved to specifically target the residues that uniquely evolved in pathogenic proteins so as to retard the emergence of drug resistance.
Large-scale conformational sampling of proteins using temperature-accelerated molecular dynamics
Authors: Abrams Cameron F. , Vanden-Eijnden Eric 
Published: March 2010

We show how to apply the method of temperature-accelerated molecular dynamics (TAMD) in collective variables [Maragliano L, Vanden-Eijnden E (2006)
Chem Phys Lett
426:168–175] to sample the conformational space of multidomain proteins in all-atom, explicitly solvated molecular dynamics simulations. The method allows the system to hyperthermally explore the free-energy surface in a set of collective variables computed at the physical temperature. As collective variables, we pick Cartesian coordinates of centers of contiguous subdomains. The method is applied to the GroEL subunit, a 55-kDa, three-domain protein, and HIV-1 gp120. For GroEL, the method induces in about 40 ns conformational changes that recapitulate the
t
→
r
′′
transition and are not observed in unaccelerated molecular dynamics: The apical domain is displaced by 30 Å, with a twist of 90° relative to the equatorial domain, and the root-mean-squared deviation relative to the
r
′′
conformer is reduced from 13 to 5 Å, representing fairly high predictive capability. For gp120, the method predicts both counterrotation of inner and outer domains and disruption of the so-called bridging sheet. In particular, TAMD on gp120 initially in the CD4-bound conformation visits conformations that deviate by 3.6 Å from the gp120 conformer in complex with antibody F105, again reflecting good predictive capability. TAMD generates plausible all-atom models of the so-far structurally uncharacterized unliganded conformation of HIV-1 gp120, which may prove useful in the development of inhibitors and immunogens. The fictitious temperature employed also gives a rough estimate of 10 kcal/mol for the free-energy barrier between conformers in both cases.
ScripTree: scripting phylogenetic graphics
Authors: Chevenet François , Croce Olivier , Hebrard Maxime , Christen Richard , Berry Vincent 
Published: March 2010

Abstract
Summary: There is a large amount of tools for interactive display of phylogenetic trees. However, there is a shortage of tools for the automation of tree rendering. Scripting phylogenetic graphics would enable the saving of graphical analyses involving numerous and complex tree handling operations and would allow the automation of repetitive tasks. ScripTree is a tool intended to fill this gap. It is an interpreter to be used in batch mode. Phylogenetic graphics instructions, related to tree rendering as well as tree annotation, are stored in a text file and processed in a sequential way.
Availability: ScripTree can be used online or downloaded at www.scriptree.org, under the GPL license.
Implementation: ScripTree, written in Tcl/Tk, is a cross-platform application available for Windows and Unix-like systems including OS X. It can be used either as a stand-alone package or included in a bioinformatic pipeline and linked to a HTTP server.
Contact: chevenet@ird.fr
SDOP-DB: a comparative standardized-protocol database for mouse phenotypic analyses
Authors: Tanaka Nobuhiko , Waki Kazunori , Kaneda Hideki , Suzuki Tomohiro , Yamada Ikuko , Furuse Tamio , Kobayashi Kimio , Motegi Hiromi , Toki Hideaki , Inoue Maki , Minowa Osamu , Noda Tetsuo , Takao Keizo , Miyakawa Tsuyoshi , Takahashi Aki , Koide Tsuyoshi , Wakana Shigeharu , Masuya Hiroshi 
Published: March 2010

Abstract
Summary: This article reports the development of SDOP-DB, which can provide definite, detailed and easy comparison of experimental protocols used in mouse phenotypic analyses among institutes or laboratories. Because SDOP-DB is fully compliant with international standards, it can act as a practical foundation for international sharing and integration of mouse phenotypic information.
Availability: SDOP-DB (http://www.brc.riken.jp/lab/bpmp/SDOP/)
Contact: hmasuya@brc.riken.jp
Supplementary information: Supplementary data are available at Bioinformatics online.
Effects of a community intervention to reduce the serving of alcohol to intoxicated patrons
Authors: Warpenius Katariina , Holmila Marja , Mustonen Heli 
Published: March 2010

ABSTRACTAimsTo assess the effects of an alcohol prevention programme to reduce the serving of alcoholic beverages to intoxicated clients on licensed premises.Research designA controlled pre‐ (2004) and post‐intervention study (2006) design.InterventionA community‐based programme combining law enforcement, responsible beverage service training, information campaigns and policy initiatives in one Finnish town (Jyväskylä).Participants and measurementsA male actor pretended to be clearly under the influence of alcohol and tried to buy a pint of beer at licensed premises. For the baseline measurement, every bar and nightclub was visited in the intervention and the control areas (94 licensed premises in total). Post‐intervention data were gathered with the same principles (100 licensed premises in total). A researcher observed every visit and documented the results.ResultsIn the post‐intervention study there was a statistically significant increase in refusals to serve denials alcohol to the actor in the intervention area (from 23% to 42% of the licensed premises) compared to refusals in the control area (from 36% to 27% of the licensed premises).ConclusionPrevious research has documented that multi‐component community‐based interventions can have a significant impact on over‐serving of alcohol when training and house policies are combined with effective law enforcement. The present findings also demonstrate that comprehensive Responsible Beverage Service (RBS) interventions applied at a local community level can be effective in decreasing service to intoxicated clients in a Nordic context.