bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proliferation and differentiation of human adipocyte precursor cells: Differences between the preperitoneal and subcutaneous compartments
Authors: Fernández M. , Acuña M.J. , Reyes M. , Olivares D. , Hirsch S. , Bunout D. , de la Maza M.P. 
Published: June 2010

AbstractHuman adipocyte precursor cells (APC) have been characterized in their proliferation and differentiation potential from subcutaneous, omental, and mesenteric depots, mostly from morbidly obese patients. Cells from the preperitoneal adipose compartment have not been characterized yet, least of all when obtained from normal weight subjects. The aim was to compare proliferation and differentiation of subcutaneous (SC) and preperitoneal (PP) APC derived from adipose tissue in healthy subjects with different body mass. SC and PP adipose tissue was obtained during surgery of inguinal hernias in five healthy non‐obese subjects and three obese otherwise healthy men. APC, obtained by collagenase digestion, were cultured. Proliferation was assayed by cell counting and differentiation by oil red O staining and flow cytometry using Nile Red staining. Proliferation of SC was higher than PP APC. Such differences between both compartments were even higher in APC obtained from obese patients. Conversely PP APC differentiated earlier in vitro compared with SC cells. These results agree with published data on fat cell proliferation. However regarding differentiation, our data show that APC from deeper depots (in this case PP) differentiate earlier than subcutaneous APC. This is different to previous studies performed in mesenteric or omental adipose tissue. J. Cell. Biochem. 111: 659–664, 2010. © 2010 Wiley‐Liss, Inc.
Elevated expression of TMEM205, a hypothetical membrane protein, is associated with cisplatin resistance
Authors: Shen Ding‐Wu , Ma Jichun , Okabe Mitsunori , Zhang Guofeng , Xia Di , Gottesman Michael M. 
Published: June 2010

AbstractDevelopment of cisplatin resistance in cancer cells appears to be a consequence of multiple epigenetic alterations in genes involved in DNA damage repair, proto‐oncogenes, apoptosis, transporters, transcription factors, etc. In this study, we found that expression of the hypothetical transmembrane protein TMEM205 (previously known as MBC3205) is associated with cisplatin resistance. TMEM205 was first detected by functional cloning from a retroviral cDNA library made from human cisplatin‐resistant (CP‐r) cells. TMEM205 is predicted to be a transmembrane protein, but its expression, localization, and function have not previously been investigated. A polyclonal antibody directed to the TMEM205 protein was raised in our laboratory. Using this antibody, it was demonstrated that this protein is located at the cell surface. Its expression is increased in our cisplatin‐selected CP‐r cell lines, as demonstrated by immunoblotting, confocal examination, and immuno‐electron microscopy. Stable transfection of the TMEM205 gene confers resistance to cisplatin by approximately 2.5‐fold. Uptake assays with Alexa Fluor‐cisplatin showed reduced accumulation in CP‐r KB‐CP.3 and KB‐CP.5 cells, and in TMEM205‐transfected cells. Analysis of TMEM205 expression profiles in normal human tissues indicates a differential expression pattern with higher expression levels in the liver, pancreas, and adrenal glands. These results indicate that a novel mechanism for cisplatin resistance is mediated by TMEM205, and also suggest that overexpression of TMEM205 in CP‐r cells may be valuable as a biomarker or target in cancer chemotherapy. J. Cell. Physiol. 225: 822–828, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis and studies on cellulose acetate—phenol formaldehyde cationic resin
Authors: Zidan F. , Metwally M. S. , Abd El‐Zahir M. 
Published: June 2010

AbstractSamples of a cationic sulphonated resin have been prepared by sulphonation of a mixture of phenol with cellulose acetate in the presence of paraformaldehyde as a crosslinking agent. The samples having average swelling percentage of 123, and a cation exchange capacity of 2.6 meq g−1 resin, are being introduced as a new catalyst in the hydrolysis of ethyl acetate ester. The synthesized cation exchanger shows a good thermal and chemical stability. Hydrolysis rate constants (kr values) for the catalysed reaction have been determined. The calculated values of the Arrhenius activation parameters show the overall slowness of the hydrolysis reaction accompanying the formation of the rigid activated complex.
Intrauterine diagnosis and management of fetal goiter: A case report
Authors: Koyuncu Faik Mumtaz , Tamay Aslı Goker , Bugday Sultan 
Published: June 2010

AbstractFetal goiter can be the result of maternal hyperthyroidism treated with antithyroid drugs. Polyhydramnios may be the presenting symptom and can be diagnosed prenatally by sonography. We report a case of fetal goiter diagnosed at 30 weeks of gestation and fetal hypothyroidism confirmed by cordocentesis. Intra‐amniotic levothyroxine was administered. Onset of preterm labor could not be prevented. The risks and benefits of intrauterine treatment of fetal goitrous hypothyroidism are discussed. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 38:503–505, 2010
Modeling of congenital erythropoietic porphyria by RNA interference: a new tool for preclinical gene therapy evaluation
Authors: Robert‐Richard Elodie , Lalanne Magalie , Lamrissi‐Garcia Isabelle , Guyonnet‐Duperat Véronique , Richard Emmanuel , Pitard Vincent , Mazurier Fréderic , Moreau‐Gaudry François , Ged Cécile , de Verneuil Hubert 
Published: June 2010

AbstractBackgroundCongenital erythropoietic porphyria (CEP) is a severe autosomal recessive disorder characterized by a deficiency in uroporphyrinogen III synthase (UROS), the fourth enzyme of the heme biosynthetic pathway. We recently demonstrated the definitive cure of a murine model of CEP by lentiviral vector‐mediated hematopoietic stem cell (HSC) gene therapy. In the perspective of a gene therapy clinical trial, human cellular models are required to evaluate the therapeutic potential of lentiviral vectors in UROS‐deficient cells. However, the rare incidence of the disease makes difficult the availability of HSCs derived from patients.MethodsRNA interference (RNAi) has been used to develop a new human model of the disease from normal cord blood HSCs. Lentivectors were developed for this purpose.ResultsWe were able to down‐regulate the level of human UROS in human cell lines and primary hematopoietic cells. A 97% reduction of UROS activity led to spontaneous uroporphyrin accumulation in human erythroid bone marrow cells of transplanted immune‐deficient mice, recapitulating the phenotype of cells derived from patients. A strong RNAi‐induced UROS inhibition allowed us to test the efficiency of different lentiviral vectors with the aim of selecting a safer vector. Restoration of UROS activity in these small hairpin RNA‐transduced CD34+ cord blood cells by therapeutic lentivectors led to a partial correction of the phenotype in vivo.ConclusionsThe RNAi strategy is an interesting new tool for preclinical gene therapy evaluation. Copyright © 2010 John Wiley & Sons, Ltd.
Empowerment through ICT education, access and use: A gender analysis of Muslim youth in India
Authors: Khan Farida , Ghadially Rehana 
Published: June 2010

AbstractInformation and communication technologies (ICTs) developing countries can bridge socio‐economic divides and empower the marginalised, including women and minority groups. This paper considers four dimensions of empowerment—psychological, social, educational and economic—and assesses benefits to each following computer education and usage of computer and Internet technology. Data were collected from 155 young Muslim women and men studying in three computer training centres in Mumbai, and a gender‐based comparison was conducted. Figures for computer ownership and home Internet connection were low for the entire sample, and the training centres and cybercafes were important points of access for females and males, respectively. In terms of perceived empowerment, young women reported higher gains than men from computer learning when combined with ICT use. Thus, despite the existence of a gender‐based digital divide, when bridged, ICTs showed potential as an equalising force between the genders. In light of the above, policy measures to widen access and provide subsidised training are suggested. Copyright © 2010 John Wiley & Sons, Ltd.
Dynamic contrast‐enhanced magnetic resonance imaging of abdominal solid organ and major vessel: Comparison of enhancement effect between Gd‐EOB‐DTPA and Gd‐DTPA
Authors: Tamada Tsutomu , Ito Katsuyoshi , Sone Teruki , Yamamoto Akira , Yoshida Koji , Kakuba Koki , Tanimoto Daigo , Higashi Hiroki , Yamashita Takenori 
Published: June 2010

AbstractPurposeTo evaluate the differences in enhancement of the abdominal solid organ and the major vessel on dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) obtained with gadolinium ethoxybenzyldiethylenetriamine pentaacetic acid (Gd‐EOB‐DTPA: EOB) and gadolinium diethylenetriamine pentaacetic acid (Gd‐DTPA) in the same patients.Materials and MethodsA total of 13 healthy volunteers underwent repeat assessments of abdominal MR examinations with DCE‐MRI using either Gd‐DTPA at a dose of 0.1 mmol/kg body weight or EOB at a dose of 0.025 mmol/kg body weight. DCE images were obtained at precontrast injection and in the arterial phase (AP: 25 seconds), portal phase (PP: 70 seconds), and equilibrium phase (EP: 3 minutes). The signal intensities (SIs) of liver at AP, PP, and EP; the SIs of spleen, renal cortex, renal medulla, pancreas, adrenal gland, aorta at AP; and the SIs of portal vein and inferior vena cava (IVC) at PP were defined using region‐of‐interest measurements, and were used for calculation of signal intensity ratio (SIR).ResultsThe mean SIRs of liver (0.195 ± 0.140), spleen (1.35 ± 0.353), renal cortex (1.58 ± 0.517), renal medulla (0.548 ± 0.259), pancreas (0.540 ± 0.183), adrenal gland (1.04 ± 0.405), and aorta (2.44 ± 0.648) at AP as well as the mean SIRs of portal vein (1.85 ± 0.477) and IVC (1.16 ± 0.187) at PP in the EOB images were significantly lower than those (0.337 ± 0.200, 1.99 ± 0.443, 2.01 ± 0.474, 0.742 ± 0.336, 0.771 ± 0.227, 1.26 ± 0.442, 3.22 ± 1.20, 2.73 ± 0.429, and 1.68 ± 0.366, respectively) in the Gd‐DTPA images (P < 0.05 each). There was no significant difference in mean SIR of liver at PP between EOB (0.529 ± 0.124) and Gd‐DTPA (0.564 ± 0.139). Conversely, the mean SIR of liver at EP was significantly higher with EOB (0.576 ± 0.167) than with Gd‐DTPA (0.396 ± 0.093) (P < 0.001).ConclusionLower arterial vascular and parenchymal enhancement with Gd‐EOB, as compared with Gd‐DTPA, may require reassessment of its dose, despite the higher late venous phase liver parenchymal enhancement. J. Magn. Reson. Imaging 2009;29:636–640. © 2009 Wiley‐Liss, Inc.
“Incivility, social undermining, bullying…oh my!”: A call to reconcile constructs within workplace aggression research
Authors: Hershcovis M. Sandy 
Published: June 2010

AbstractResearch in the field of workplace aggression has rapidly developed in the last two decades, and with this growth has come an abundance of overlapping constructs that fall under the broad rubric of workplace aggression. While researchers have conceptually distinguished these constructs, it is unclear whether this proliferation of constructs is adding appreciably to our knowledge, or whether it is constraining the questions we ask. In this paper, I consider five example constructs (i.e., abusive supervision, bullying, incivility, social undermining, and interpersonal conflict) and argue that the manner in which we have differentiated these (and other) aggression constructs does not add appreciably to our knowledge of workplace aggression. I then provide supplementary meta‐analytic evidence to show that there is not a predictable pattern of outcomes from these constructs, and propose a restructuring of the manner in which we conceptualize workplace aggression. Copyright © 2010 John Wiley & Sons, Ltd.
Regulation of apoptosis in nucleus pulposus cells by optimized exogenous Bcl‐2 overexpression
Authors: Sudo Hideki , Minami Akio 
Published: June 2010

AbstractAlthough the etiology of intervertebral disc degeneration is poorly understood, one possible approach to regulate the process of intervertebral disc degeneration may include the inhibition of apoptosis. We investigated the anti‐apoptotic effects of bcl‐2 in nucleus pulposus cells to enhance disc cell survival. Rat nucleus pulposus cells were transfected in vitro with a codon optimized rat bcl‐2 gene. Forty‐eight hours after transfection, cells were cultured in serum‐deprived medium. After serum withdrawal, the cells were evaluated for bcl‐2 protein levels and cell apoptosis. To investigate the effects of bcl‐2 overexpression on the final apoptotic pathways and on basic genes important for nucleus pulposus homeostasis, mRNA levels of caspase‐3, type II collagen, and aggrecan were also quantified. Nucleus pulposus cells were successfully transfected with codon optimized bcl‐2 gene, which effectively reduced serum starvation‐induced cell apoptosis. Overexpression of bcl‐2 also reduced the mRNA expression level of caspase‐3. mRNA levels of type II collagen and aggrecan were significantly higher in bcl‐2 transfected groups compared to control plasmid vector groups after serum withdrawal. We firstly showed that bcl‐2 overexpression in intervertebral disc cells was effective in preventing in vitro apoptotic cell death, indicating the potential advantages of this therapeutic approach in regulating disc degeneration. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1608–1613, 2010
Role of mechanical loading in healing of massive bone autografts
Authors: Tate Melissa L. Knothe , Dolejs Scott , Miller R. Matthew , Knothe Ulf R. 
Published: June 2010

AbstractWe assessed healing of a 3.5 cm autograft transport segment, denuded of periosteum, and docked to the healthy distal femur with an intramedullary nail. We hypothesized that healing relates to proximity to the healthy distal femur and to mechanical loading patterns. Total bone area, area of new bone apposition, and quality of new bone formed in the 2 weeks after surgery, and area and degree of perfusion 16 weeks after surgery were measured as a function of proximity and loading patterns (as defined by the major and minor centroidal axes, CA). At 16 weeks, no significant differences in early bone apposition or perfusion were observed as a function of distance from the healthy distal femur. Qualitatively, bone was well perfused, both vascularly and pericellularly, and highly remodeled. When cross‐sections were pooled from distal to proximal through the docking zone and normalized for total bone area, significant differences in the amount of early proliferative woven bone were related to loading patterns. In contrast, no differences in normalized perfusion area were attributable to loading patterns. Furthermore, early bone apposition and perfusion decreased with increasing radial distance from the bone surface toward the intramedullary nail. Finally, no differences were observed in areas of resorption within the docking zone compared to baseline levels measured in the control (in bone removed to create the defect zone at the time of surgery). Interestingly, infilling of resorption spaces within docking zone specimens related significantly to predominant loading patterns, where areas within the major CA exhibited significantly more infilling. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1657–1664, 2010
Preoperative clinically inapparent leucopenia in patients undergoing neoadjuvant chemotherapy for locally advanced gastric cancer is not a risk factor for surgical or general postoperative complications
Authors: Reim Daniel , Hüser Norbert , Humberg Daniela , Novotny Alexander , Assfalg Volker , Matevossian Edouard , Friess Helmut , Schuhmacher Christoph 
Published: June 2010

AbstractBackgroundSince the recent MAGIC trial neoadjuvant chemotherapy has been considered as treatment option for patients with advanced gastric cancer for tumor‐downsizing and increasing R0 resection rates. Morbidity was reported in 45% of the patients treated within this randomized trial. Due to myelotoxicity under chemotherapy a part of the patients might undergo surgery with preoperative leucopenia. As leucopenia causes adverse events such as opportunistic infections and fever, it might be considered as a relevant risk factor in the course of surgical treatment.Patients and MethodsWe analyzed a cohort of neoadjuvantly treated patients (n = 214), which had a clinically inapparent but proven leucopenia (n = 58) before undergoing surgery due to advanced stage gastric cancer.ResultsStatistical analysis by Fisher's exact test showed, that there was no significant effect neither on general (P = 0.191) nor on surgery‐dependant postoperative complications (P = 0.75).ConclusionConclusively patients with clinically inapparent leucopenia after neoadjuvant chemotherapy due to advanced stage gastric cancer can be safely operated on without putting them in danger of relevant surgical complications. J. Surg. Oncol. 2010;102:321–324. © 2010 Wiley‐Liss, Inc.
Effects of preceding interferon therapy on outcome after surgery for hepatitis C virus‐related hepatocellular carcinoma
Authors: Tomimaru Yoshito , Nagano Hiroaki , Eguchi Hidetoshi , Kobayashi Shogo , Marubashi Shigeru , Wada Hiroshi , Tanemura Masahiro , Umeshita Koji , Hiramatsu Naoki , Takehara Tetsuo , Doki Yuichiro , Mori Masaki 
Published: June 2010

AbstractBackground and ObjectivesInterferon (IFN) can eradicate hepatitis C virus (HCV)‐RNA from serum and hepatic tissue, and suppress the development of hepatocellular carcinoma (HCC). Despite such effectiveness, HCC develops even in HCV patients successfully treated with IFN therapy.MethodsHCV‐related HCC patients who underwent curative hepatectomy for HCC were divided into three groups according to preceding IFN for HCV infection therapy and the therapeutic effect: responders group (n = 23), non‐responders group (n = 46), and no‐IFN group (n = 215). Postoperative outcome was retrospectively examined in the three groups.ResultsAST and ALT were significantly lower in responders group than non‐responders group (P < 0.001, P = 0.001) and no‐IFN group (P = 0.001, P = 0.002). Platelet count was significantly higher in responders group than other groups (P = 0.008, P = 0.001). The percentage of cirrhotic patients in responders group was significantly lower than other groups (P = 0.017, P = 0.014). Multivariate analysis identified preceding IFN therapy to be associated with disease‐free survival at marginal significance (P = 0.086), and as a significant independent factor for overall survival (P = 0.042).ConclusionsPreceding IFN therapy for HCV infection improves postoperative outcome in HCV‐related HCC patients treated successfully with IFN. J. Surg. Oncol. 2010;102:308–314. © 2010 Wiley‐Liss, Inc.
VEGF and cortactin expression are independent predictors of tumor recurrence following curative resection of gastric cancer
Authors: Wang Xin , Cao Weiguo , Mo Mingcong , Wang Wei , Wu Huita , Wang Jiejun 
Published: June 2010

AbstractIntroductionTo investigate the clinicopathological role of expression of vascular endothelial growth factor (VEGF) and cortactin, as well as whether their expression are independent predictors of tumor recurrence following curative resection of gastric cancer.MethodsOne hundred twenty‐eight patients with gastric cancer were included in this study. Formalin‐fixed paraffin‐embedded specimens were stained for VEGF and cortactin, and the correlation between the staining, clinicopathological parameters and prognostic power were analyzed.ResultsOf the 128 patients studied, 58 (45.3%) and 71 (55.5%) cases were strongly positive for VEGF and cortactin, respectively. VEGF expression correlated with Lauren classification (P < 0.001), pathological tumor stage (P < 0.001), and pathological tumor node metastasis (TNM) stage (P = 0.003). Cortactin expression correlated with pathological lymph node stage (P = 0.018), pathological TNM stage (P < 0.001), and degree of differentiation (P < 0.001). There were statistically significant associations between tumor recurrence and VEGF expression (P = 0.023), and cortactin expression (P < 0.001). In multivariate analysis, pathological TNM stage, VEGF expression, and cortactin expression were independent prognostic influence on disease‐free survival (P < 0.001, 0.022, and 0.034, respectively).ConclusionsVEGF and cortactin may be a good biomarker to be applied in clinic to predict the prognosis of patients with curatively resected gastric cancer. J. Surg. Oncol. 2010;102:325–330. © 2010 Wiley‐Liss, Inc.
Comparison of common mobile‐phase volume markers with polar‐group‐containing reversed‐phase stationary phases
Authors: Perry Paige R. , Coym Jason W. 
Published: June 2010

AbstractA systematic study of the behavior of several common mobile‐phase volume markers using traditional and polar‐group‐containing reversed‐phase stationary phases is presented. Examined mobile‐phase volume markers include two neutral molecules, uracil and thiourea, concentrated (0.10 M) and dilute (0.0001 M) KNO3, and D2O. Mobile‐phase volumes are examined over the entire reversed‐phase mobile‐phase range of 100% water to 100% methanol or acetonitrile. The behavior of these mobile‐phase volume markers is compared with a maximum theoretical value (i.e. the void volume), as determined by pycnometry. The data suggest that: (i) uracil begins to fail as a mobile‐phase volume marker in mobile phases below about 40% strong solvent for polar group containing phases; (ii) in nearly all cases, the mobile‐phase volume measured dynamically is smaller than the pycnometric void volume; (iii) a significant dependence of measured mobile‐phase volume on salt concentration is seen on the polar endcapped phase, which is not observed on the traditional and embedded polar group phase; and (iv) D2O does not work well as a mobile‐phase volume marker with polar‐group‐containing phases, possibly due to interaction with the stationary phase polar group.