bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression profile of genes identified in human spermatogonial stem cell‐like cells using suppression subtractive hybridization
Authors: Yoo Jung Ki , Lim Jung Jin , Ko Jung Jae , Lee Dong Ryul , Kim Jin Kyeoung 
Published: April 2010

AbstractSpermatogenesis is the process by which testicular spermatogonial stem cells (SSCs) self‐renew and differentiate into mature sperm in the testis. Maintaining healthy spermatogenesis requires proper proliferation of SSCs. In this study, we sought to identify factors that regulate the proliferation of SSCs. Human SSC (hSSC)‐like cells were isolated from azoospermic patients by a modified culture method and propagated in vitro. After four to five passages, the SSC‐like cells spontaneously ceased proliferating in vitro, so we collected proliferating (P)‐hSSC‐like cells at passage two and senescent (S)‐hSSC‐like cells at passage five. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed between the P‐hSSC‐like and S‐hSSC‐like cells. We selected positive clones up‐regulated in P‐hSSC‐like cells using SSH and functionally characterized them by reference to public databases using NCBI BLAST tools. Expression levels of genes corresponding to subtracted clones were analyzed using RT‐PCR. Finally, we confirmed the differential expression of 128 genes in positive clones of P‐hSSC‐like cells compared with S‐hSSC‐like cells and selected 23 known and 39 unknown clones for further study. Known genes were associated with diverse functions; 22% were related to metabolism. Fifteen of the known genes and two of the unknown genes were down‐regulated after senescence of hSSC‐like cells. A comparison with previous reports further suggests that known genes selected, SPP1, may be related to germ cell biogenesis and cellular proliferation. Our findings identify several potential novel candidate biomarkers of proliferating‐ and senescencet‐hSSCs, and they provide potentially important insights into the function and characteristics of human SSCs. J. Cell. Biochem. 110: 752–762, 2010. © 2010 Wiley‐Liss, Inc.
Lyn kinase and ZAP70 are substrates of PTPROt in B‐cells: Lyn inactivation by PTPROt sensitizes leukemia cells to VEGF‐R inhibitor pazopanib
Authors: Motiwala Tasneem , Datta Jharna , Kutay Huban , Roy Satavisha , Jacob Samson T. 
Published: April 2010

AbstractWe have recently shown that the gene encoding the truncated form of protein tyrosine phosphatase receptor‐type O (PTPROt) expressed predominantly in hematopoietic cells is epigenetically silenced in human primary chronic lymphocytic leukemia (B‐CLL). To determine whether increased phosphorylation of the PTPROt substrates following PTPROt suppression alters signal transduction pathway(s) that impart a growth advantage to the leukemic lymphocytes, it is critical to discern the key substrates of PTPROt. Here, we used substrate‐trapping assay to identify two novel substrates of PTPROt, the tyrosine kinases Lyn and ZAP70. Both Lyn and ZAP70 were dephosphorylated by wild‐type PTPROt, but not by its catalytic site (CS) mutant. A critical phosphorylation site in Lyn, Y397, essential for its activity was dephosphorylated by PTPROt. Consequently, the activity of Lyn kinase was compromised when co‐expressed with PTPROt‐WT compared to vector control or upon co‐expression with PTPROt‐CS. Ectopic expression of PTPROt in Raji cells reduced phosphorylation of Lyn in the absence of any change in its protein levels. These results have revealed the physiological importance of PTPROt in regulating B‐cell receptor signaling at Lyn kinase. Further, ectopic expression of PTPROt also sensitized the cells to the VEGF‐R inhibitor Pazopanib. J. Cell. Biochem. 110: 846–856, 2010. © 2010 Wiley‐Liss, Inc.
The relationship between bone mechanical properties and ground reaction forces in normal and hypermuscular mice
Authors: Schmitt Daniel , Zumwalt Ann C. , Hamrick Mark W. 
Published: April 2010

AbstractUnderstanding the relationship between external load and bone morphology is critical for understanding adaptations to load in extant animals and inferring behavior in extinct forms. Yet, the relationship between bony anatomy and load is poorly understood, with empirical studies often producing conflicting results. It is widely assumed in many ecological and paleontological studies that bone size and strength reflect the forces experienced by the bone in vivo. This study examines that assumption by providing preliminary data on gait mechanics in a hypermuscular myostatin‐deficient mouse model with highly mineralized and hypertrophied long bones. A small sample of hypermuscular and wild‐type mice was video recorded while walking freely across a force platform. Temporal gait parameters, peak vertical and transverse (mediolateral) ground reaction forces (GRFs), vertical impulse, and loading rates were measured. The only gait parameters that differed between the two groups were the speeds at which the animals traveled and the transverse forces on the hind limb. The myostatin‐deficient mice move relatively slowly and experienced the same magnitude of vertical forces on all limbs and transverse forces on the forelimb as the wild‐type mice; though the myostatin‐deficient mice did experience lower mediolateral forces on their hindlimbs compared with the wild‐type mice. These preliminary results call into question the hypothesis that skeletal hypertrophy observed in hypermuscular mice is a result of larger GRFs experienced by the animals' limbs during locomotion. This calls for further analysis and a cautious approach to inferences about locomotor behavior derived from bony morphology in extant and fossil species.J. Exp. Zool. 313A:339–351, 2010. © 2010 Wiley‐Liss, Inc.
Asymmetric [3H]‐labeling using ruthenium catalyzed transfer hydrogenation
Authors: Arns Steve , Moreau Anne , Young Robert N. 
Published: April 2010

AbstractThe first general method for asymmetric tritiation using ruthenium catalyzed transfer hydrogenation and its application to the radiosynthesis of an exemplary prostaglandin EP4 receptor‐selective Prostaglandin E2 analog is described. The methodology should be of general utility for the enantioselective preparation of α‐tritiated chiral secondary alcohols. Copyright © 2010 John Wiley & Sons, Ltd.
Synapse loss in dementias
Authors: Clare Ryan , King Victoria G. , Wirenfeldt Martin , Vinters Harry V. 
Published: April 2010

AbstractSynaptic transmission is essential for nervous system function, and its dysfunction is a known major contributing factor to Alzheimer's‐type dementia. Antigen‐specific immunochemical methods are able to characterize synapse loss in dementia through the quantification of various synaptic proteins involved in the synaptic cycle. These immunochemical methods applied to the study of Alzheimer's disease (AD) brain specimens have correlated synaptic loss with particularly toxic forms of amyloid‐β protein and have also established synapse loss as the best correlate of dementia severity. A significant but comparatively circumscribed amount of literature describes synaptic decline in other forms of dementia. Ischemic vascular dementia (IVD) is quite heterogeneous, and synapse loss in IVD seems to be variable among IVD subtypes, probably reflecting its variable neuropathologic correlates. Loss of synaptic protein has been identified in vascular dementia of the Binswanger type and Spatz‐Lindenberg's disease. Here we demonstrate a significant loss of synaptophysin density within the temporal lobe of frontotemporal dementia (FTD) patients. © 2010 Wiley‐Liss, Inc.
Enhanced adenoviral gene delivery to motor and dorsal root ganglion neurons following injection into demyelinated peripheral nerves
Authors: Zhang Yongjie , Zheng Yiyan , Zhang Yi Ping , Shields Lisa B.E. , Hu Xiaoling , Yu Panpan , Burke Darlene A. , Wang Heming , Jun Cai , Byers Jonathan , Whittemore Scott R. , Shields Christopher B. 
Published: April 2010

AbstractInjection of viral vectors into peripheral nerves may transfer specific genes into their dorsal root ganglion (DRG) neurons and motoneurons. However, myelin sheaths of peripheral axons block the entry of viral particles into nerves. We studied whether mild, transient peripheral nerve demyelination prior to intraneural viral vector injection would enhance gene transfer to target DRG neurons and motoneurons. The right sciatic nerve of C57BL/6 mice was focally demyelinated with 1% lysolecithin, and the left sciatic nerve was similarly injected with saline (control). Five days after demyelination, 0.5 μl of Ad5‐GFP was injected into both sciatic nerves at the site of previous injection. The effectiveness of gene transfer was evaluated by counting GFP+ neurons in the DRGs and ventral horns. After peripheral nerve demyelination, there was a fivefold increase in the number of infected DRG neurons and almost a 15‐fold increase in the number of infected motoneurons compared with the control, nondemyelinated side. Focal demyelination reduced the myelin sheath barrier, allowing greater virus–axon contact. Increased CXADR expression on the demyelinated axons facilitated axoplasmic viral entry. No animals sustained any prolonged neurological deficits. Increased gene delivery into DRG neurons and motoneurons may provide effective treatment for amyotrophic lateral sclerosis, pain, and spinal cord injury. © 2010 Wiley‐Liss, Inc.
Impairment of nuclear factor‐κB activation increased glutamate excitotoxicity in a motoneuron–neuroblastoma hybrid cell line expressing mutant (G93A) Cu/Zn‐superoxide dismutase
Authors: Pyo Jung‐Soo , Ko Young San , Kim Woo Ho , Kim Manho , Lee Kwang‐Woo , Nam Seon Young , Chung Hee‐Yong , Cho Sung Jin , Baik Tai‐Kyoung , Lee Byung Lan 
Published: April 2010

AbstractMutations in the superoxide dismutase 1 (SOD1) gene are linked to glutamate excitotoxicity in familial amyotrophic lateral sclerosis (fALS), but the underlying mechanism remains unclear. We investigated whether nuclear factor‐κB (NF‐κB) activation is involved in glutamate excitotoxicity by using motor neuron–neuroblastoma hybrid cells that expressed a mutant (G93A) SOD1 (mtSOD1) or wild‐type SOD1 (wtSOD1). MtSOD1 cells were more vulnerable to glutamate excitotoxicity than wtSOD1 cells and showed higher NF‐κB activity, higher nuclear cRel expression, and lower nuclear RelA expression under basal conditions. Glutamate treatment increased NF‐κB activation along with nuclear expressions of RelA and cRel in wtSOD1 cells but induced only weak nuclear RelA expression in mtSOD1 cells. Suppression of NF‐κB activation using transfection of the superrepressive mutant form of IκBα (mIκBα) inhibited nuclear RelA expression in both types of SOD1 cells, which increased glutamate excitotoxicity in wtSOD1 cells but not in mtSOD1 cells. Furthermore, immunohistochemistry confirmed stronger RelA immunoreactivity in the nuclei of motor neurons of spinal cord in wild‐type SOD1 transgenic mice than in those in SOD1 G93A transgenic mice. In addition, we found that glutamate treatment decreased XIAP expression and increased caspase‐3 activity in mtSOD1 cells and mIκBα‐overexpressing wtSOD1 cells. Our results suggest that glutamate excitotoxicity in motor neurons of SOD1‐linked fALS is attributable, at least in part, to the impairment of IκBα‐dependent RelA activation and subsequent apoptosis mediated by XIAP inhibition and caspase‐3 activation. © 2010 Wiley‐Liss, Inc.
Prognostic indicators in node‐negative advanced gastric cancer patients
Authors: Saito Hiroaki , Kuroda Hirohiko , Matsunaga Tomoyuki , Fukuda Kenji , Tatebe Shigeru , Tsujitani Shunichi , Ikeguchi Masahide 
Published: April 2010

AbstractBackground and ObjectivesDespite carrying better overall prognoses, some node‐negative gastric cancer patients die from recurrent malignancies. Identifying factors associated with disease‐specific survival in adequately staged node‐negative gastric cancer is important, as these patients are presumably free of microscopic regional metastases and may derive significant benefit from existing or future adjuvant strategies.MethodsTo investigate significant prognostic indicators in node‐negative advanced gastric cancer patients, we reviewed 777 advanced gastric cancer patients who had undergone curative gastrectomies.ResultsThe 5‐year survival rate of node‐negative advanced gastric cancer patients is 84.9%, which is significantly better than that of patients with lymph node metastasis. Multivariate analysis indicated that tumor size, histology, and depth of invasion are independent prognostic factors. The 5‐year survival rate of patients with larger tumors (≥7 cm), poorly differentiated adenocarcinoma, and serosal invasion was 49.1%, which was significantly worse that of patients with fewer or none of these factors.ConclusionsTumor size, histology, and the presence of serosal invasion are strong indicators of poor prognosis in node‐negative advanced gastric cancer patients. J. Surg. Oncol. 2010; 101:622–625. © 2010 Wiley‐Liss, Inc.
Abductor paralysis after botox injection for adductor spasmodic dysphonia
Authors: Venkatesan Naren N. , Johns Michael M. , Hapner Edie R. , DelGaudio John M. 
Published: April 2010

AbstractObjectives/Hypothesis:Botulinum toxin (Botox) injections into the thyroarytenoid muscles are the current standard of care for adductor spasmodic dysphonia (ADSD). Reported adverse effects include a period of breathiness, throat pain, and difficulty with swallowing liquids. Here we report multiple cases of bilateral abductor paralysis following Botox injections for ADSD, a complication previously unreported.Study Design:Retrospective case series.Methods:Patients that received Botox injections for spasmodic dysphonia between January 2000 and October 2009 were evaluated. Patients with ADSD were identified. The number of treatments received and adverse effects were noted. For patients with bilateral abductor paralysis, age, sex, paralytic Botox dose, prior Botox dose, and course following paralysis were noted.Results:From a database of 452 patients receiving Botox, 352 patients had been diagnosed with ADSD. Of these 352 patients, eight patients suffered bilateral abductor paralysis, and two suffered this complication twice. All affected patients were females over the age of 50 years. Most patients had received treatments prior to abductor paralysis and continued receiving after paralysis. Seven patients recovered after a brief period of activity restrictions, and one underwent a tracheotomy. The incidence of abductor paralysis after Botox injection for ADSD was 0.34%.Conclusions:Bilateral abductor paralysis is a rare complication of Botox injections for ADSD, causing difficulty with breathing upon exertion. The likely mechanism of paralysis is diffusion of Botox around the muscular process of the arytenoid to the posterior cricoarytenoid muscles. The paralysis is temporary, and watchful waiting with restriction of activity is the recommended management. Laryngoscope, 2010
A corrosion study of nickel–copper and nickel–copper–palladium welding filler metals
Authors: Liang D. , Sowards J. W. , Frankel G. S. , Alexandrov B. T. , Lippold J. C. 
Published: April 2010

AbstractIn an effort to reduce the release of fumes containing carcinogenic Cr6+ during arc welding of stainless steel, Cr‐free filler metals for welding of SS304 have been developed. Corrosion studies were carried out on 304L stainless steel samples welded with these Cr‐free consumables. The corrosion properties of gas tungsten arc (GTA) and shielded metal arc (SMA) welds fabricated with NiCu and NiCuPd consumables were found to be comparable to those of welds fabricated with SS308L, the standard filler metal used with SS304. Although the breakdown potentials of the welds made using both welding processes were lower than that of the SS308L GTA weld, the repassivation potentials of these welds were much higher. Generally, the repassivation potential is a more conservative measure of susceptibility to localized corrosion. Accordingly, the NiCu and NiCuPd welds were more resistant to crevice corrosion than SS308L welds. The addition of a small amount of Pd improved the corrosion resistance relative to NiCu welds, which is consistent with previous studies from specially‐prepared button samples and bead‐on‐plate samples. Other corrosion studies such as creviced and uncreviced long time immersion, atmospheric exposure, and slow strain rate testing suggest that NiCuPd filler metal can be a potential replacement for the conventional SS308L filler metal for joining SS304.
Choline kinase overexpression increases invasiveness and drug resistance of human breast cancer cells
Authors: Shah Tariq , Wildes Flonne , Penet Marie‐France , Winnard Paul T. , Glunde Kristine , Artemov Dmitri , Ackerstaff Ellen , Gimi Barjor , Kakkad Samata , Raman Venu , Bhujwalla Zaver M. 
Published: April 2010

AbstractA direct correlation exists between increased choline kinase (Chk) expression, and the resulting increase of phosphocholine levels, and histological tumor grade. To better understand the function of Chk and choline phospholipid metabolism in breast cancer we have stably overexpressed one of the two isoforms of Chk‐α known to be upregulated in malignant cells, in non‐invasive MCF‐7 human breast cancer cells. Dynamic tracking of cell invasion and cell metabolism were studied with a magnetic resonance (MR) compatible cell perfusion assay. The MR based invasion assay demonstrated that MCF‐7 cells overexpressing Chk‐α (MCF‐7‐Chk) exhibited an increase of invasion relative to control MCF‐7 cells (0.84 vs 0.3). Proton MR spectroscopy studies showed significantly higher phosphocholine and elevated triglyceride signals in Chk overexpressing clones compared to control cells. A test of drug resistance in MCF‐7‐Chk cells revealed that these cells had an increased resistance to 5‐fluorouracil and higher expression of thymidylate synthase compared to control MCF‐7 cells. To further characterize increased drug resistance in these cells, we performed rhodamine‐123 efflux studies to evaluate drug efflux pumps. MCF‐7‐Chk cells effluxed twice as much rhodamine‐123 compared to MCF‐7 cells. Chk‐α overexpression resulted in MCF‐7 human breast cancer cells acquiring an increasingly aggressive phenotype, supporting the role of Chk‐α in mediating invasion and drug resistance, and the use of phosphocholine as a biomarker of aggressive breast cancers. Copyright © 2010 John Wiley & Sons, Ltd.
Four‐dimensional echocardiography with B‐flow imaging and spatiotemporal image correlation in the assessment of congenital heart defects
Authors: Zhang Ming , Pu Da‐Rong , Zhou Qi‐Chang , Peng Qing‐Hai , Tian Lei‐Qi 
Published: April 2010

AbstractObjectiveThe objective of the present study was to evaluate the role of four‐dimensional ultrasound with B‐flow imaging and spatiotemporal image correlation (STIC) in the detection of congenital heart defects.MethodsAutomated longitudinal and transverse sweeping of the fetal chest was utilized to obtain the volume datasets of the heart. Among the fetuses recruited in the study, 20 were normal and 13 were fetuses with congenital heart anomalies. Reconstructed images of abnormal hearts were compared with those of normal fetuses.ResultsOf the 20 normal fetuses, all extracardiac vessels such as aorta, pulmonary artery, ductus artery, inferior vena cava, and ductus venosus could be detected on reconstructed images of 16 fetuses. In five normal cases, a four‐dimensional image was recorded to allow simultaneous visualization of all four pulmonary veins. In the 13 fetuses with cardiac anomalies, four‐dimensional sonography with B‐flow imaging and STIC detected the ‘digital casts’ of the outflow tracts, great arteries, and veins draining into the heart. These results demonstrate spatial relationship among these structures which provide important anatomical information.ConclusionsThe use of four‐dimensional ultrasound examination with B‐flow imaging and STIC is a very useful technique in identifying anatomical features of different congenital cardiac anomalies. Copyright © 2010 John Wiley & Sons, Ltd.
Assessing self‐efficacy for coping with cancer: development and psychometric analysis of the brief version of the Cancer Behavior Inventory (CBI‐B)
Authors: Heitzmann Carolyn A. , Merluzzi Thomas V. , Jean‐Pierre Pascal , Roscoe Joseph A. , Kirsh Kenneth L. , Passik Steven D. 
Published: April 2010

AbstractObjective: The Cancer Behavior Inventory‐Brief Version (CBI‐B), a 12‐item measure of self‐efficacy for coping with cancer derived from the longer 33‐item version, was subjected to psychometric analysis.Method: Participants consisted of three samples: 735 cancer patients from a multicenter CCOP study, 199 from central Indiana, and 370 from a national sample. Samples were mixed with respect to initial cancer diagnosis. Participants completed the CBI‐B and measures of quality of life, optimism, life satisfaction, depression, and sickness impact.Results: Exploratory Factor Analysis with oblique rotation yielded four factors in the first sample: (1) Maintaining Independence and Positive Attitude; (2) Participating in Medical Care; (3) Coping and Stress Management; and (4) Managing Affect, which were confirmed in subsequent samples. Cronbach α coefficient for the 12‐item CBI‐B ranged from 0.84 to 0.88. Validity of the CBI‐B was demonstrated by positive correlations with measures of quality of life and optimism, and negative correlations with measures of depression and sickness impact.Conclusion: The CBI‐B is a valid brief measure of self‐efficacy for coping that could be easily integrated into clinical oncology research and practice, and also used in screening patients. Copyright © 2010 John Wiley & Sons, Ltd.
The supportive care needs survey for partners and caregivers of cancer survivors: development and psychometric evaluation
Authors: Girgis Afaf , Lambert Sylvie , Lecathelinais Christophe 
Published: April 2010

AbstractObjective: Begin to test the psychometric properties of a measure designed to capture the multi‐dimensional supportive care needs of cancer caregivers: the Supportive Care Needs Survey—Partners and Caregivers (SCNS‐P&C).Methods: Draft SCNS‐P&C items were developed with reference to the literature and existing instruments and reviewed for face and content validity. The final SCNS‐P&C was then completed by 547 cancer caregivers. Psychometric analyses conducted included principal factor analysis, internal consistency, and construct validity through the known‐group approach.Results: Factor analysis revealed four domains of needs: Health Care Service Needs, Psychological and Emotional Needs, Work and Social Needs, and Information Needs, with Cronbach's α coefficients ranging from 0.88 to 0.94. Construct validity of the SCNS‐P&C was partially supported. Across all domains, individuals with anxiety or depression were more likely to report at least one unmet moderate or high need in comparison to non‐anxious or non‐depressed participants. A greater proportion of younger participants experienced at least one unmet moderate or high need within the Psychological and Emotional Needs and Work and Social Needs domains. Proportion of reported unmet needs varied across cancer types for the Health Care Service Needs and Information Needs domains.Conclusions: The SCNS‐P&C has the potential to comprehensively assess the range of caregivers' supportive care needs, across the illness trajectory. Analyses supported the tool's internal consistency and construct validity. The SCNS‐P&C can be used by researchers and clinicians to determine caregivers' unmet needs, prioritise health‐care resources, and tailor supportive cancer care services accordingly. Copyright © 2010 John Wiley & Sons, Ltd.