bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Efficacy of Various Interventions to Reduce Perceived Stress Among Older Adults: A Systematic Review and Network Meta‐Analysis
Authors: Zhu Mingyue , Chen Huimin , Wang Qing , Ding Xiaotong , Li Zheng 
Published: February 2025

ABSTRACTBackgroundExisting interventions targeting perceived stress in older adults exhibit a diverse range of types and inconsistent effectiveness. It remains unclear which interventions are most effective for older adults in terms of perceived stress, anxiety, depression, and cortisol levels.AimsThis study aimed to assess the effectiveness of interventions targeting perceived stress in older adults for perceived stress, depression, anxiety, and cortisol levels.MethodsA systematic search was conducted using PubMed, EMBASE, Web of Science, Cochrane, CINAHL, PsycINFO, CNKI, SinoMed, VIP, and WanFang databases on January 9, 2024 for randomized controlled trials (RCTs). Pairwise meta‐analysis and network meta‐analysis (NMA) were conducted using Review Manager v.5.4 and Stata v.16.0.ResultsA total of 23 RCTs were included. The reminiscence therapy had the highest probability (98.6%) of being the most promising intervention to reduce perceived stress, followed by exercise (68.1%) and yoga (56.1%). The included interventions had benefits on cortisol levels (SMD = −0.30; 95% CI [−0.54, −0.06]; p = 0.01). Moreover, exercise showed positive effects on reducing depression (SMD = −1.84; 95% CI [−3.69, 0.01]; p = 0.05), and it was ranked as the most promising method for depression or anxiety. Health education also reduced anxiety symptoms compared to control group (SMD = −0.77; 95% CI [−1.27, −0.26]; p = 0.03).Linking Evidence to ActionInterventions targeting perceived stress had overall benefits in reducing perceived stress in older adults, especially reminiscence therapy and exercise. Exercise and health education each had potential benefits for alleviating depression and anxiety, respectively. More high‐quality RCTs are needed to obtain more robust conclusions.
Multi-objective structural optimization of hydraulically controllable reciprocating seals with comprehensive static and dynamic performance
Authors: Li Xiaoxuan , Wang Bingqing , Li Yuntang , Peng Xudong , Chen Yuan , Li Xiaolu 
Published: February 2025

Abstract
The hydraulically controllable reciprocating seal (HCRS) is a novel intelligent sealing technology capable of online performance regulation, making it one of the preferred solutions for highly reliable seals in the industrial field. However, the impact of structural parameters on the performance of it remains unclear, complicating efforts to provide precise guidance for structural design. To address these issues, this paper first employs a mixed thermoelastohydrodynamic lubrication (TEHL) model to perform a parametric analysis of how structural parameters affect the static and dynamic performance of seals, identifying key influencing factors. Secondly, a multi-objective optimization model was established to derive the optimal structural design using a comprehensive balance method. Finally, the performance results before and after optimization were compared. The results indicate that the width of the slide ring, the air side angle of the slide ring, the inner diameter of the slide ring, and the length of the internal pressure cavity are critical factors influencing sealing performance. Following optimization, the maximum von Mises stress in the seal was reduced by 51.5%, net leakage decreased by 12.9%, and friction power loss reduced by 2.25%. This demonstrates that the optimization method is effective for designing seals with low leakage, low friction, and high reliability.
Gelling and reducing agents are potential carbon and energy sources in culturing of anaerobic microorganisms
Authors: Liu Yi-Fan , Yang Liu , He Qing-Ping , Xu Yi-Lin , Zhu Yu-Tong , Mi Yan-Le , Zhou Lei , Yang Shi-Zhong , Gu Ji-Dong , Mu Bo-Zhong 
Published: February 2025

ABSTRACT

The majority of microorganisms in the environment have yet to be isolated in pure cultures, and the reasons behind this phenomenon remain elusive. In this study, we investigated the possibility of the commonly used gelling agent including agar and gellan gum as a source of carbon and energy in anaerobic roll-tube cultivation from one mangrove sediment sample and two high-temperature oilfield samples. Based on growth tests and genomic evidence, anaerobic gellan degraders were retrieved from genera of
Clostridium
,
Lacrimispora
, and lineages from the rarely cultivated phylum Atribacterota. Anaerobic agarolytic microorganisms were found to be members of
Bacillus
and
Clostridium
. We also proved the role of carbon and energy sources of L-cysteine, a routine agent used to make culture media anoxic/anaerobic in both enrichment cultures and isolated strains representing
Acetomicrobium
,
Thermodesulfovibrio
,
Lacrimispora
,
Clostridium
,
Bacillus
,
Coprothermobacter
,
Citrobacter
, and
Enterobacter
. Furthermore, the isolates and enriched microbial communities utilizing L-cysteine under anaerobic conditions were mainly through L-cysteine desulfuration to pyruvate, ammonia, and sulfide. This study demonstrates that the widely used gelling and reducing agents in the basal medium can serve as carbon and energy sources for anaerobic microorganisms and thus may bias the enrichment and isolation.

IMPORTANCE
Most microbial species inhabiting natural environments have not been isolated in pure cultures using conventional media and laboratory conditions, but the reason behind this is unclear. Here, we provided a new explanation for the phenomenon, in that both the gelling agents, like agar and gellan gum, and reducing agent L-cysteine-HCl in the media provide extra carbon and energy sources to microorganisms and therefore decrease the chance in isolation specifically for the supplemented substrate which is supposed to be the sole source of carbon and energy. This result demonstrated that further improvement in the effectiveness of isolation of targeted microorganisms will be facilitated by subtracting the overlooked organic ingredients in the medium and more innovations.
Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
Authors: Furusawa Yuri , Kiso Maki , Uraki Ryuta , Sakai-Tagawa Yuko , Nagai Hiroyuki , Koga Michiko , Kashima Yukie , Hojo Masayuki , Iwamoto Noriko , Iwatsuki-Horimoto Kiyoko , Ohmagari Norio , Suzuki Yutaka , Yotsuyanagi Hiroshi , Halfmann Peter J. , Kamitani Wataru , Yamayoshi Seiya , Kawaoka Yoshihiro 
Published: February 2025

ABSTRACT

In general, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates well at 37°C, which is the temperature of the human lower respiratory tract, but it poorly at 30°C‒32°C, which is the temperature of the human upper respiratory tract. The replication efficiency of SARS-CoV-2 in the upper respiratory tract may directly affect its transmissibility. In this study, an XBB.1.5 isolate showed superior replicative ability at 32°C and 30°C, whereas most other Omicron sub-variant isolates showed limited growth. Deep sequencing analysis demonstrated that the frequencies of viruses possessing the NSP6-S163P and NSP13-P238S substitutions increased to more than 97% during propagation of the XBB.1.5 isolate at 32°C but did not reach 55% at 37°C. Reverse genetics revealed that these substitutions contributed to superior virus growth
in vitro
at these low temperatures by improving virus genome replication. Mutant virus possessing both substitutions showed slightly higher virus titers in the upper respiratory tract of hamsters compared to the parental virus; however, transmissibility between hamsters was similar for the mutant and parental viruses. Taken together, our findings indicate that NSP6-S163P and NSP13-P238S contribute to superior virus growth at low temperatures
in vitro
and in the upper respiratory tract of hamsters.

IMPORTANCE
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates efficiently at 37°C. However, the temperature of the human upper airway is 30°C–32°C. Therefore, the replicative ability of SARS-CoV-2 at low temperatures could influence virus replication in the upper airway and transmissibility. In this study, we assessed the growth of Omicron sub-variants at low temperatures and found that an XBB.1.5 isolate showed increased replicative ability. By deep sequencing analysis and reverse genetics, we found that amino acid changes in NSP6 and NSP13 contribute to the low-temperature growth; these changes improved RNA polymerase activity at low temperatures and enhanced virus replication in the upper airway of hamsters. Although these substitutions alone did not drastically affect virus transmissibility, in combination with other substitutions, they could affect virus replication in humans. Furthermore, since these substitutions enhance virus replication in cultured cells, they could be used to improve the production of inactivated or live attenuated vaccine virus.
Characterization of intestinal fungal communities through ITS amplicon from metagenomic libraries of sea cucumber (
Holothuria glaberrima
)
Authors: Paez-Diaz Jessica Alejandra , Nieves-Morales Rene , Melendez-Martinez Gabriela , Rivera-Lopez Edwin Omar , Rodríguez-Ramos Josué , Rios-Velazquez Carlos 
Published: February 2025

ABSTRACT

The brown rock sea cucumber (
Holothuria glaberrima
) microbiome is vital for understanding its ecological dynamics. This study focuses on the microbial composition and abundance of fungi across different intestinal regions of
H. glaberrima,
giving insights into host-microbe interactions and supporting future ecological and comparative studies on their regeneration capabilities.
Vitamin C protects against doxorubicin induced skeletal muscle atrophy: Role of oxidative stress
Authors: Filho Antonio Viana do Nascimento , Stoyell-Conti Filipe Fernandes , Gauri Akolkar , Lima Lara Susan Silva , Bernardes Nathalia , Irigoyen Maria Claudia , Singal Pawan , De Angelis Kátia , Dias Danielle da Silva 
Published: February 2025

Doxorubicin is known for its significant cardiotoxicity, driven in part by elevated oxidative stress (OS). Furthermore, preclinical models have demonstrated that doxorubicin induces skeletal muscle atrophy. While vitamin C has been recognized as a valuable pharmacological intervention for mitigating cardiac toxicity, its impact on doxorubicin-induced skeletal muscle atrophy is still to be determined. Therefore, this study aimed to investigate the effects of vitamin C on the skeletal muscle of rats subjected to doxorubicin exposure. Indeed, doxorubicin led to a reduction in body weight and gastrocnemius muscle weight. It also increased H2O2, protein oxidation and lipid peroxidation in the gastrocnemius. On the other hand, vitamin C was able to prevent the loss in skeletal muscle mass, as well as, the increase in markers of oxidative stress. In addition, negative correlations between gastrocnemius muscle mass and markers of cell damage were found. In conclusion, vitamin C emerges as a protective agent against doxorubicin-induced skeletal muscle atrophy and oxidative stress. This suggests its potential application as a prophylactic measure for patients undergoing doxorubicin treatment. Keywords: Doxorubicin, Vitamin C, Oxidative Stress, Skeletal Muscle.
The Protective Effects of Sivelestat Sodium on the Basis of Corticosteroid Therapy in Patients With Moderate‐to‐Severe Acute Respiratory Distress Syndrome
Authors: Ma Yujie , Tang Guofu , Liu Xiaotong , Gao Qiang 
Published: February 2025

Objective: We aimed to evaluate the protective effects of sivelestat sodium on the basis of corticosteroid therapy in patients with moderate‐to‐severe acute respiratory distress syndrome (ARDS).Methods: We retrospectively investigated 127 patients with confirmed moderate‐to‐severe ARDS treated in the intensive care unit (ICU) at Dazhou Central Hospital. Patients were divided into the control group (corticosteroids alone) and the combination therapy of steroids and sivelestat sodium (CTSSS) group according to the therapeutic interventions. The primary outcome was in‐hospital mortality. And the baseline characteristics and laboratory findings of patients were collected for analysis.Results: The overall mortality rate in 127 patients was 48.8%. There was no statistically significant difference in in‐hospital mortality between the CTSSS group and the control group (45.3% vs. 56.1%). In the subgroup of patients aged < 80 years or with an Acute Physiology and Chronic Health Evaluation (APACHE) II score < 30, CTSSS could reduce the risk of mortality (odds ratio [OR] = 0.41, 95% confidence interval [CI], 0.17–0.96, p = 0.041; OR = 0.31, 95% CI, 0.13–0.77, p = 0.012; respectively). Among patients aged 80 years or older, those with CTSSS exhibited a significantly elevated risk of mortality (OR = 13; 95% CI, 1.20–140.73; p = 0.035).Conclusion: Compared with corticosteroids alone, CTSSS could improve oxygenation index, increase lymphocyte count, protect extrapulmonary organs and reduce in‐hospital mortality rate in patients with moderate‐to‐severe ARDS in specific subgroups (age < 80 years or APACHE II score < 30). It might be advisable to avoid CTSSS in moderate‐to‐severe ARDS patients aged 80 years or older. Prospective studies involving larger sample sizes are needed to verify these findings.
Intensive Care Unit Nurses’ Organizational Climate and Clinical Competency in Saudi Government Hospitals: Cross‐Sectional and Multicenter Study
Authors: Villagracia Hazel Novela , Alanezi Asma Jamel , Alshammri Ruqaih Zawher , Alblwei Khlood Hamdan , Harbi Fahad Falah Al , Alanezi Faisal Mohammed , Alanazi Saif Mohammed , Berdida Daniel Joseph E. , Grande Rizal Angelo N. , Villagracia Rico William A. 
Published: February 2025

Aims and Objectives: To investigate the connection between organizational climate and clinical competency among intensive care unit (ICU) nurses in government hospitals in Saudi Arabia.Background: Across the Middle Eastern healthcare settings, studies that explored the link between organizational climate and clinical competency among ICU nurses remain underreported.Methods: This cross‐sectional study involved 206 ICU nurses from seven government hospitals in the Ha’il Region, Saudi Arabia. Two validated self‐report scales were utilized to gather data from August to September 2023. Descriptive and inferential statistics were used to analyze the data.Results: Most ICU nurses were female, aged 31–35 years, married, with a Bachelor of Science in Nursing degree, and had 4–6 years of ICU experience. The organizational climate was perceived positively by 98.10% of nurses, with the highest mean score for independent practice and support. The clinical competency mean score was at a middle level, with the highest mean score for clinical care. Significant differences were noted between organizational climate and age, marital status, educational attainment, years of experience, and hospital assigned. Also, significant differences were demonstrated between clinical competency and gender, marital status, and daily patient workload. Finally, there was a positive correlation between organizational climate and clinical competency.Conclusion: The study revealed a positive perception of organizational climate among ICU nurses, with a moderate level of clinical competency. The hospital administrators and policymakers can focus on the enhancement of the organizational climate to further support and develop ICU nurses’ competencies.Implications for Nursing Management: Nurse managers and healthcare administrators should cultivate a positive organizational climate and enhance clinical competency to ensure nursing care quality and improved client outcomes among ICU nurses, thereby promoting commitment, retention, and professional relationships of the workforce.
Stabilization of RUNX1 Induced by O-GlcNAcylation Promotes PDGF-BB-Mediated Resistance to CDK4/6 Inhibitors in Breast Cancer
Authors: Zhou Shuyan , Zhang Yi , Belmar Julie , Hou Chunyan , Zhang Yaqin , Peng Changmin , Meng Yunxiao , Li Zhuqing , Mughal Muhammad Jameel. , Gao Yanjun , Seto Edward , Shen Min , Hall Matthew D. , Ma Junfeng , Ma Cynthia X. , Li Shunqiang , Zhu Wenge 
Published: February 2025

Abstract
Cyclin-dependent kinases 4 and 6 (CDK4/6) are crucial in regulating cell cycle progression and cancer development. Targeting CDK4/6 has shown considerable promise in treating various cancers, including breast cancer. Despite significant therapeutic efficacy, resistance to CDK4/6 inhibitors (CDK4/6i), such as palbociclib, remains a substantial hurdle in clinical practice. Using a co-culture system, cytokine array, and quantitative high-throughput combinatorial screening (qHTCS), we discovered a mechanism by which the RUNX1-PDGF-BB axis regulates palbociclib resistance in breast cancer cells. Specifically, RUNX1 functioned as a transcription factor to drive expression of PDGF-BB, leading to resistance to palbociclib by enhancing the Akt pathway and suppressing senescence. Furthermore, in resistant cells, RUNX1 was O-GlcNAcylated at serine 252 (S252) by OGT, resulting in the stabilization of RUNX1 by preventing ubiquitin-mediated degradation. Inhibition of the RUNX1-PDGF-BB axis by specific inhibitors overcame palbociclib resistance both in vitro and in vivo. Notably, the RUNX1-PDGF-BB axis was upregulated in resistant patient-derived xenograft (PDX) lines and in breast cancer patients following treatment with CDK4/6i. These findings not only unveil O-GlcNAcylation-mediated activation of a RUNX1-PDGF-BB pathway as a driver of palbociclib resistance but also provide clinical evidence supporting the repurposing of FDA-approved PDGFR inhibitors as a therapeutic strategy to treat CDK4/6i-resistant breast cancer patients.
Cancer and Overgrowth Manifestations of PTEN Hamartoma Tumour Syndrome: Management Recommendations from the International PHTS Consensus Guidelines Working Group
Authors: Dhawan Andrew , Baitamouni Sarah , Liu Darren , Yehia Lamis , Anthony Kristin , McCarther Alicia , Tischkowitz Marc , MacFarland Suzanne P. , Ngeow Joanne , Hoogerbrugge Nicoline , Eng Charis 
Published: February 2025

Abstract
Purpose: PTEN hamartoma tumour syndrome (PHTS) is an autosomal dominant cancer-predisposition and overgrowth syndrome occurring due to pathogenic germline variants in the PTEN gene, with an increased risk of both benign and malignant tumours involving the breast, colon, endometrium, thyroid, skin, and kidney. The objective of these clinical guidelines was to use the latest knowledge to generate an international consensus resource for providers, researchers, and individuals with PHTS on the best practices in the surveillance and management of cancer and overgrowth in PHTS. Experimental Design: The International PHTS Cancer and Overgrowth Guidelines Working Group was established, comprising a core group of six international experts in the diagnosis and management of PHTS. The Working Group held joint meetings with PHTS individuals and their advocates. Informed by the literature, the Working Group met regularly between 2022 and 2024 to produce guideline statements, refined through iterative feedback. A modified Delphi approach was used with an independent external panel of PHTS, genetics and cancer experts to establish final consensus guidelines. Results: Clinical consensus recommendations for the surveillance and management of cancer and overgrowth in individuals with PHTS were formed. Guidelines encompass the recommended practices in cases of breast, colon, endometrial, thyroid, and kidney cancers, as well as overgrowths. Conclusions: The clinical management of individuals with PHTS is complex and necessitates a multidisciplinary approach. We generated international consensus guidelines for the surveillance and management of cancer and overgrowth in PHTS aiming at improving care for affected individuals and families.
Figure 7 from A Melanoma Brain Metastasis CTC Signature and CTC:B-cell Clusters Associate with Secondary Liver Metastasis: A Melanoma Brain–Liver Metastasis Axis
Authors: Bowley Tetiana Y. , Ortiz Mireya C. , Lagutina Irina V. , Steinkamp Mara P. , Fahy Bridget N. , Tawfik Bernard , Harari-Turquie Moises , Marchetti Dario 
Published: February 2025

<p>Spatial single-cell gene expression analysis of CTC-derived cell:B-cell interactions employing 10x Genomics Xenium platform (<a href="http://www.10xgenomics.com/" target="_blank">www.10xgenomics.com</a>). HuNBSGW A2.1 mice were engrafted with CTC-derived clonal cells via intracardiac injection (70W-SM3-Luc2; 5.0 × 10<sup>5</sup> cells/animal). <b>A,</b> Dim plot shows 2D scatter plot representing cell clusters in reduced space. Cell annotations are shown on the right. Brain (<b>B</b>, top left) and liver (<b>B</b>, top right) metastases of second-generation CDXs were isolated, paraffin-embedded, sectioned, and analyzed via the 10x Xenium platform. An image of the whole tissue section of huNBSGW brain (<b>B</b>) and huNBSGW liver is presented (left), followed by two close-up images (right). CTC-derived melanoma cells were identified as S100A1<sup>+</sup> cells (red), whereas B cells were defined as BCR<sup>+</sup> cells (green). Only melanoma cells showed extensive RPL23<sup>+</sup> and RPL35A<sup>+</sup> gene expression as significant members of the RPL/RPS CTC signature (<a href="#bib31" target="_blank">31</a>). Bottom, quantitation of percentage and number of S100<sup>+</sup> and BCR<sup>+</sup> cells/mm<sup>2</sup>. See “Materials and Methods” for experimental details. BM, bone marrow; UMAP, Uniform Manifold Approximation and Projection; CMP, common myeloid progenitor; iPS, induced pluripotent stem cell; MEP, Megakaryocyte–erythroid progenitor cell; MSC, mesenchymal stem cell.</p>
ВАГІТНІСТЬ ТА НЕДИФЕРЕНЦІЙОВАНА ДИСПЛАЗІЯ СПОЛУЧНОЇ ТКАНИНИ
Authors: Римарчук М. І. , Островська О. М. , Кишакевич І. Т. , Геник Н. І. , Перхулин О. М. 
Published: February 2025

Метa дослідження – встановити частоту маркерів недиференційованої дисплазії сполучної тканини (нДСТ) при акушерських та перинатальних ускладненнях та оцінити патогенетичну роль магнієвого дефіциту як фактора порушення регуляції метаболізму сполучної тканини.
Матеріали та методи. Проведено аналіз історії пологів у 240 жінок віком (32,6±2,4) року, які були поділені на дві групи: 160 пацієнток з фенотипом нДСТ (основна група) та 80 пацієнток контрольної групи з фізіологічним перебігом вагітності без маркерів нДСТ.
Результати дослідження та їх обговорення. Оцінка «перинатального портрета» дозволила розкрити комплекс численних симптомів, серед яких: дефіцит маси тіла жінки при народженні (24,4 %), народження від передчасних пологів (19,4 %), підвищений тягар кардіоваскулярних, цереброваскулярних патологічних станів та репродуктивних втрат у сімейному анамнезі таких жінок (21,3 %). Маркери нДСТ характеризувалися вираженою варіабельністю і представлені найбільш поширеними фенотиповими особливостями. Спектр патології вагітності і пологів дозволив вказати на високий відсоток передчасного розриву навколоплодових оболонок (25,6 %), стрімких пологів або аномалії пологової діяльності (19,4 %), недоношування (16,9 %), патологічної плацентації (24,4 %), материнського травматизму, резистентної до терапії, гіпотонічної дисфункції матки (13,1 %), що можна віднести до так званих «акушерських» маркерів нДСТ. Встановлено гіпомагнезіємію у випадку нДСТ.
Висновки. Таким чином, можна стверджувати, що запрограмовані предиктори порушення сполучної тканини при нДСТ ініціюють недостатню спроможність до повноцінного забезпечення гестаційних перетворень (інвазія трофобласта, модифікація спіральних артерій, судинна адаптація), що супроводжується невиношуванням вагітності і розвитком гестаційної та перинатальної патології. Результати дослідження демонструють переконливі свідчення, що зі слабкістю сполучної тканини асоційована матково-плацентарна апоплексія (OR – 5,68; р<0,004), істміко-цервікальна недостатність (OR – 17,49, р<0,001), неповноцінність рубця на матці (OR – 8,73; р<0,04) та акушерський травматизм (OR – 6,55; р<0,04). Констатовано знижений рівень мікроколагеноспецифічних біоелементів – дефіцит концентрації магнію, що сприяє вираженню та реалізації спадкових та набутих дефектів сполучної тканини при нДСТ.