bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implant surgery using bone‐ and mucosa‐supported stereolithographic guides in totally edentulous jaws: surgical and post‐operative outcomes of computer‐aided vs. standard techniques
Authors: Arısan Volkan , Karabuda Cüneyt Z. , Özdemir Tayfun 
Published: May 2010

AbstractObjectives: The aim of this study was to compare the surgical and post‐operative outcomes of a computer‐aided implant surgery performed by bone‐ and mucosa‐supported stereolithographic (SLA) guides against the standard technique.Material and methods: Multiple‐ and single‐type SLA guides from two commercial manufacturers were produced and a total of 341 implants were placed to 52 patients using the standard technique (Control group), bone‐ (bone‐supported guide [BSG] group) and mucosa‐supported SLA guides (Flapless group) in 21, 16 and 15 patients, respectively. Surgical duration (min), number of analgesics (tablets) as well as hemorrhage, difficulty in mouth opening (or trismus) and other incidences were recorded. Pain and swelling was assessed using the visual analog scale (VAS). Parametric and non‐parametric tests were used for statistical analysis (P<.05).Results: The mean surgery duration (23.53±5.48 min) and the number of analgesics consumed (four tablets) in the Flapless group were lower than those in the control (68.71±11.4 min and 10 tablets) and BSG groups (60.94±13.07 min and 11 tablets, P<0.01). The change in pain scores (VAS) and the number of analgesics consumed in time were statistically significant (P<0.01 and 0.05, respectively) and the Flapless group reported a lower pain score than the BSG (P<0.01) and Control groups (P<0.001). The Flapless group experienced less hemorrhage (χ2=4.12, P=0.041 on the day of surgery) and fewer instances of trismus (χ2=6.91, P=0.031 the day after surgery). The differences in early‐term failures were not statistically significant between the groups (log‐rank test: P=0.782).Conclusion: The use of mucosa‐supported single SLA guides for flapless implant placement may help reduce the surgery duration, pain intensity, related analgesic consumption and most other complications typical in the post‐implant surgery period. However, there are particular drawbacks in both guide types and further studies are required to confirm the prosthodontic conformity and long‐term success of implants placed using computer‐assisted techniques. To cite this article:
Arısan V, Karabuda CZ, Özdemir T. Implant surgery using bone‐ and mucosa‐supported stereolithographic guides in totally edentulous jaws: surgical and post‐operative outcomes of computer‐aided vs. standard techniques. Clin. Oral Impl. Res. 21, 2010; 980–988. doi: 10.1111/j.1600‐0501.2010.01957.x
Effects of non‐surgical periodontal therapy on the glomerular filtration rate of the kidney: an exploratory trial
Authors: Graziani Filippo , Cei Silvia , La Ferla Fabio , Vano Michele , Gabriele Mario , Tonetti Maurizio 
Published: May 2010

Graziani F, Cei S, La Ferla F, Vano M, Gabriele M, Tonetti M. Effects of non‐surgical periodontal therapy on glomerular filtration rate of the kidney: an exploratory trial. J Clin Periodontol 2010; 37: 638–643. doi: 10.1111/j.1600‐051X.2010.01578.x.AbstractObjective: To determine whether non‐surgical periodontal treatment (PT) would exert, in subjects with generalized chronic periodontitis (GCP), some beneficial effect on renal function as indicated by surrogate measures of the glomerular filtration rate (GFR).Material and Methods: Twenty GCP systemically healthy subjects were treated with PT. Serum samples were collected at baseline and 1 day, 7, 30, 90 and 180 days after treatment. GFR was evaluated using cystatin C, a serum marker and modification of diet in renal disease (MDRD), an equation involving creatinine, urea and albumin. Serum markers of systemic inflammation such as C‐reactive protein (CRP), D‐dimer, serum amyloid A (SAA) and fibrinogen were also assessed.Results: The cystatin C level decreased significantly from baseline to the end of the trial (p<0.01). Conversely, MDRD did not vary. A significant inflammatory reaction was produced by PT in the short term. Greater increases were noted for CRP and SAA within 24 h (p<0.001 versus baseline), while d‐dimer (p<0.05) and fibrinogen (p<0.01) showed mild variations. The values of inflammatory markers were normalized after 30 days.Conclusions: GFR, as assessed by cystatin C levels, may be positively affected by PT. Because of the exploratory nature of this trial, further research is needed to investigate this preliminary finding.
Predictors of tooth loss during long‐term periodontal maintenance: a systematic review of observational studies
Authors: Chambrone Leandro , Chambrone Daniela , Lima Luiz A. , Chambrone Luiz A. 
Published: May 2010

Chambrone L, Chambrone D, Lima LA, Chambrone LA. Predictors of tooth loss during long‐term periodontal maintenance: a systematic review of observational studies. J Clin Periodontol 2010; 37: 675–684. doi: 10.1111/j.1600‐051X.2010.01587.xAbstractObjective: To systematically assess the factors influencing tooth loss during long‐term periodontal maintenance (PM).Methods: CENTRAL, MEDLINE and EMBASE were searched up to and including September 2009. Studies limited to patients with periodontitis who underwent periodontal therapy and followed a maintenance care programme for the at least 5 years were eligible for inclusion in this review. Studies were considered for inclusion if they reported data on tooth loss during PM.Results: The search strategy identified 527 potentially eligible articles, of which 13 retrospective case series were included in this review. The risk of bias assessment evaluated by the Newcastle–Ottawa scale showed that eight studies were considered of medium methodological quality and five of low methodological quality. Of 41,404 teeth present after active periodontal treatment, 3919 were lost during PM. The percentages of tooth loss due to periodontal reasons and of patients who did not experience tooth loss varied from 1.5% to 9.8% and 36.0% to 88.5%. Studies' individual outcomes showed that different patient‐related factors (i.e. age and smoking) and tooth‐related factors (tooth type and location, and the initial tooth prognosis) were associated with tooth loss during PM.Conclusions: The considerable heterogeneity found among studies did not allow definitive conclusions. Age, smoking and initial tooth prognosis were found to be associated with tooth loss during PM. Overall, patients must be instructed to follow periodic PM and quit smoking (smokers). Prospective cohort studies are required to confirm the possible predictors of tooth loss due to periodontal reasons. The allocation of patients into subgroups according to the type of periodontitis and smoking frequency will allow more accurate evaluations.
CK7 expression in primary cutaneous squamous cell carcinoma
Authors: Pulitzer Melissa , Desman Garrett , Busam Klaus J. 
Published: May 2010

Aim: To evaluate cytokeratin 7 (CK7) immunoreactivity in invasive primary cutaneous squamous cell carcinomas (SCCs). Methods: Twenty‐seven primary cutaneous SCCs from 25 patients were evaluated for tumor grade using hematoxylin and eosin‐stained slides and for percentage and intensity of immunoreactivity for CK7. All cases exhibited features of SCC with an in situ component. No glandular or tubular differentiation was present. Staining intensity was graded on a scale of 0–3, with 0 indicating no reaction. Of immunoreactive cases, percentage of tumor staining and distribution of immunoreactivity was documented. Results: Six of 27 SCCs (22%) exhibited immunostaining for CK7. Of those cases, three were poorly differentiated, exhibiting 2 to 3+ intensity in 5–15% of cells. Two were poorly differentiated, with 2 to 3+ intensity in 30–60% of cells. The remaining immunoreactive tumor was moderately differentiated, with 1+ intensity and 5% staining in an area of microinvasion. Conclusion: A subset of cutaneous SCCs, in particular, poorly differentiated tumors, may show focal‐to‐partial immunoreactivity for CK7. This is important to bear in mind when immunohistochemistry is used to distinguish SCC from simulants, such as porocarcinoma, or other adnexal carcinomas with squamous metaplasia.Pulitzer M, Desman G, Busam KJ. CK7 expression in primary cutaneous squamous cell carcinoma.
HA modulation of epidermal morphogenesis in an organotypic keratinocyte‐fibroblast co‐culture model
Authors: Gu Hua , Huang Lin , Wong Yim‐Ping , Burd Andrew 
Published: May 2010

Please cite this paper as: HA modulation of epidermal morphogenesis in an organotypic keratinocyte‐fibroblast co‐culture model. Experimental Dermatology 2010; 19: e336–e339.Abstract:  Hyaluronan (HA) is a major extracellular matrix component of the skin. Amongst its biological functions is the maintenance of epidermal homeostasis. The mechanisms of action, however, remain unclear. To explore the interaction of HA with the epidermis, we have looked at the effects of exogenous application of HA in an organotypic culture model containing a dermal substrate with and without fibroblast incorporation. The results demonstrate that exogenous HA enhances epidermal proliferation resulting in a thicker viable epidermis with an increase in the number and intensity of Ki67‐positive basal cells; HA also improves the basement membrane assembly as evidenced by an increased expression of laminin‐332 and collagen type IV but not the expression of Nidogen‐1 at the epidermal–dermal junctional zone; furthermore, the development of epidermal lipid barrier structure was enhanced. These findings provide evidence to support the therapeutic use of exogenous HA for treating skin disorders with aberrant epidermal homeostasis.
Deposition of chromatin‐IgG complexes in skin of nephritic MRL‐lpr/lpr mice is associated with increased local matrix metalloprotease activities
Authors: Hedberg Annica , Fismen Silje , Fenton Kristin A. , Mortensen Elin S. , Rekvig Ole P. 
Published: May 2010

Please cite this paper as: Deposition of chromatin‐IgG complexes in skin of nephritic MRL‐lpr/lpr mice is associated with increased local matrix metalloprotease activities. Experimental Dermatology 2010; 19: e265–e274.Abstract:  Chromatin‐IgG complexes appear as electron dense structures (EDS) in glomerular basement membranes in lupus nephritis. Here, we present results of comparative analyses of the composition of EDS in murine lupus dermatitis and nephritis. One focus was to perform an analytical approach to understand why such complex structures bind skin basement membrane components. Transcription of skin membrane‐encoding genes was analysed to see if expression of such genes was increased, eventually indicating that binding capacity of immune complexes increased when dermatitis developed. Variations in matrix metalloprotease 2 (MMP2), MMP9 and Dnase1 mRNA levels and enzymatic activities were correlated with circulatory chromatin‐IgG complexes and deposition in skin. We also examined if glomerular deposits of EDS predicted similar deposits in skin of (NZB × NZW)F1 or MRL‐lpr/lpr mice, as we observed chromatin‐IgG complexes in capillary lumina in skin and glomeruli in both strains. EDS consisting of chromatin fragments and IgG were found sub‐epidermally in skin with LE‐like lesions of end‐stage nephritic MRL‐lpr/lpr mice. Dermal MMP‐encoding genes were up‐regulated during disease progression, and gelatinolytic activity was increased in affected skin. Dnase1 mRNA level and total nuclease activity remained stable in skin during the disease, in contrast to progressive loss of renal Dnase1 mRNA and total renal nuclease activity during development of nephritis. Loss of renal Dnase1 may explain release of chromatin fragments, while increased MMP activity may disrupt membranes making them accessible for chromatin fragment‐IgG complexes. Circulatory chromatin‐IgG complexes, and up‐regulated intradermal MMP activity may be crucial for deposition of immune complexes in skin of lupus‐prone mice.
Functional characterization of highly adherent CD34+ keratinocytes isolated from human skin
Authors: Gutiérrez‐Rivera Araika , Pavón‐Rodríguez Andrés , Jiménez‐Acosta Francisco , Poblet Enrique , Braun Kristin M. , Cormenzana Pedro , Ciria Juan Pablo , Larretxea Rafael , Cárdenas José Manuel , Izeta Ander 
Published: May 2010

Please cite this paper as: Functional characterization of highly adherent CD34+ keratinocytes isolated from human skin. Experimental Dermatology 2010; 19: 685–688.Abstract:  Compared to murine models, data on cells responsible for the homeostasis of human epidermis are scarce and often contradictory. Given the conflicting results and the availability of clinical grade protocols to purify CD34 cells from a given tissue, we pursued to phenotypically characterize human epidermal CD34+ population. After magnetic separation of whole skin CD34+ and CD34− cell fractions and selection for cells highly adherent to extracellular matrix, both CD34± fractions retained the ability to form a stratified epidermis in organotypic cultures and presented similar in vitro migratory phenotypes. However CD34− cells showed higher clonogenic potential and in vitro proliferative capacity. These results indicated that CD34− cell fraction contains stem/early progenitor cells, while CD34+ cells might be a transit‐amplifying precursor for hair follicle (HF) sheath cells. The ability to isolate living cells using differential cell adhesion and surface markers provides an opportunity to study cells from different morphological regions of the HF.
Splenic angioleiomyoma in an owl monkey (Aotus nancymae)
Authors: Gozalo Alfonso S. , Zerfas Patricia M. , Starost Matthew F. , Elkins William R. , Clarke Carol L. 
Published: May 2010

AbstractBackground  An adult male owl monkey (Aotus nancymae) underwent a splenectomy. When the spleen was removed, a small, nodular mass slightly bulging over the splenic surface was noted.Methods  The mass was examined by light and transmission electron microscopy and by immunohistochemistry.Results  On light microscopy, the mass was well‐circumscribed, non‐encapsulated, and composed of haphazardly arranged smooth muscle bundles admixed with numerous small capillary‐like structures containing blood. Immunohistochemical (IHC) staining revealed the tumor was strongly positive for smooth muscle actin yielding vascular smooth muscle bundles, and for Factor VIII, staining endothelial cells within the smooth muscle bundles. Transmission electron microscopy (TEM) showed a large portion of the cells to be atypical appearing smooth muscle and a few cells had structures resembling Weibel–Palade bodies indicating endothelial cells.Conclusions  Based on cell morphology, by light and TEM, and IHC a final diagnosis of splenic angioleiomyoma was made. This is, to our knowledge, the first report of an angioleiomyoma in a non‐human primate.
Antigen‐presenting Cells in Pregnant and Non‐pregnant Human Myometrium
Authors: Ivanisevic Marina , Segerer Sabine , Rieger Lorenz , Kapp Michaela , Dietl Johannes , Kämmerer Ulrike , Frambach Torsten 
Published: May 2010

Citation Ivanisevic M, Segerer S, Rieger L, Kapp M, Dietl J, Kämmerer U, Frambach T. Antigen‐presenting cells in pregnant and non‐pregnant human myometrium. Am J Reprod Immunol 2010; 64: 188–196Problem  Inflammatory cells play a crucial role in human parturition. Different populations of leucocytes invade the reproductive tract. Numerous studies have described the decidual immune cell population in pregnant and non‐pregnant endometrium. However, little is known about the presence of immune cells in human myometrium.Method of study  We herein analysed a spectrum of immune cells in human myometrium comparing tissue samples from non‐pregnant (n = 8) and pregnant (n = 10) uteri. Applying immunohistochemistry with a panel of antibodies specific for T cells, monocytes, natural killer cells, B cells and antigen‐presenting cells (CD4, CD8, CD14, CD15, CD16, CD19, CD56, CD68, CD83, HLA‐DR, DC‐Sign, mast cell tryptase), we characterized the immune cell population of human myometrium.Results  A significantly higher number of CD14, CD15, CD16, DC‐SIGN as well as CD4‐positive cells were found in myometrium of pregnant compared to non‐pregnant uteri, while mast cells were significantly reduced in pregnant myometrium.Conclusion  All markers found increased in pregnant myometrium indicate monocyte/macrophage lineage cells and thus suggest a possible involvement of these cells in healthy pregnancy maintenance. Monocytes/macrophages might produce a microenvironment that permits a controlled invasion of trophoblast cells into the myometrium while preventing a rejection of the semiallogenic conceptus and providing an important barrier against invading pathogenes.
Neonatal blood glucose concentrations in caesarean and vaginally delivered term infants
Authors: Marom Ronella , Dollberg Shaul , Mimouni Francis B. , Berger Irit , Mordechayev Nina , Ochshorn Yifat , Mandel Dror 
Published: May 2010

AbstractBackground:  Little is known about the glucose concentrations at and after birth of infants delivered by caesarean section (CS), when compared with infants born vaginally (VD).Aim:  To compare venous cord blood glucose concentrations of term infants born after elective CS to infants born by VD. We studied the null hypothesis that mode of delivery does not affect neonatal blood glucose values.Methods:  We compared cord blood glucose concentrations in healthy term infants born after VD (n = 16) or by elective CS (n = 21). Glucose concentrations were obtained immediately at birth from the umbilical cord. Kruskal–Wallis was used to compare glucose concentrations and demographic variables between the groups.Results:  Gestational age was 39.6 ± 0.8 weeks in VD group vs. 38.7 ± 0.9 weeks in CS group, and birthweight was 3359 ± 494 vs. 3500 ± 528 g. Cord blood glucose concentration was higher in VD (81.3 ± 16.9 mg/dL) than CS infants (70.3 ± 9.7 mg/dL, p = 0.039). The change in blood glucose concentration over the first 2‐h of life differed significantly between the two groups, being an increase in CS versus a decrease in VD infants (−3.5 ± 15.2 vs. −15.4 ± 24.6 mg/dL, p = 0.013).Conclusions:  Glucose concentrations in VD infants are higher than in infants born by elective CS without labour.
Impact of flooding on feeding practices of infants and young children in Dhaka, Bangladesh Slums: what are the coping strategies?
Authors: Goudet Sophie M. , Griffiths Paula L. , Bogin Barry A. , Selim Nasima 
Published: May 2010

AbstractPrevious research has shown that urban slums are hostile environments for the growth of infants and young children (IYC). Flooding is a hazard commonly found in Dhaka slums (Bangladesh) which negatively impacts IYC's nutritional and health status. This paper aims 1) to identify the impact of flooding on IYC's feeding practices, and 2) to explore the coping strategies developed by caregivers. Qualitative data (participant observation and semi‐structured interviews) and quantitative data (household questionnaire and anthropometric measurements) collected in slums in Dhaka (n = 18 mothers,n = 5 community health workers, andn = 55 children) were analysed. The subjects of the interviews were mothers and Bangladesh Rural Advancement Committee (BRAC) community health workers living and working in the slums. Research findings showed that breastfeeding and complementary feeding practices for IYC were poor and inappropriate due to lack of knowledge, time, and resources in normal times and worse during flooding. One coping strategy developed by mothers purposely to protect their IYC's nutritional status was to decrease their personal food intake. Our research findings suggest that mothers perceived the negative impact of flooding on their IYC's nutritional health but did not have the means to prevent it. They could only maintain their health through coping strategies which had other negative consequences. The results suggests a holistic approach combining 1) provision of relief for nutritionally vulnerable groups during flooding, 2) support to mothers in their working role, 3) breastfeeding counseling and support to lactating mothers with difficulties, and 4) preventing malnutrition in under 2 year old children.
Human δ opioid receptor biogenesis is regulated via interactions with SERCA2b and calnexin
Authors: Tuusa Jussi T. , Leskelä Tarja T. , Petäjä‐Repo Ulla E. 
Published: May 2010

Sarco(endo)plasmic reticulum calcium ATPase (SERCA)2b maintains the cellular Ca2+ homeostasis by transferring Ca2+ from the cytosol to the lumen of the endoplasmic reticulum (ER). Recently, SERCA2b has also been shown to be involved in the biosynthesis of secreted and membrane proteins via direct protein–protein interactions, involving components of the ER folding and quality‐control machinery, as well as newly synthesized G protein‐coupled receptors. Here we demonstrate that the human delta opioid receptor (hδOR) exists in a ternary complex with SERCA2b and the ER molecular chaperone calnexin. The interaction between SERCA2b and hδOR in vivo did not require calnexin as it was independent of the C‐terminal calnexin‐interacting domain of SERCA2b. However, the receptor was able to mediate co‐immunoprecipitation of calnexin with the C‐terminally truncated SERCA2b. The association of SERCA2b with hδOR was regulated in vitro by Ca2+ and ATP in a manner that was opposite to the calnexin–hδOR interaction. Importantly, co‐expression of the catalytically inactive SERCA2b(D351A) or calnexin binding‐compromised SERCA2bΔC mutants with the receptor decreased the expression of mature receptors in a manner that did not directly relate to changes in the ER Ca2+ concentration. We conclude that dynamic interactions among SERCA2b, calnexin and the hδOR precursor orchestrate receptor biogenesis and are regulated by Ca2+ and ATP. We further hypothesize that the primary role of SERCA2b in this process is to act as a Ca2+ sensor in the vicinity of active translocons, integrating protein folding with local fluctuations of ER Ca2+ levels.Structured digital abstract
MINT‐7889371: hδORΔC (uniprotkb:P41143) physically interacts (MI:0915) with SERCA2b (uniprotkb:P16615) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7889483: hδOR (uniprotkb:P41143) physically interacts (MI:0914) with Calnexin (uniprotkb:P27824) and SERCA2b (uniprotkb:P16615) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7889460: SERCA2b (uniprotkb:P11607) physically interacts (MI:0914) with hδOR (uniprotkb:P41143) and Calnexin (uniprotkb:P27824) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7889389: SERCA2b (uniprotkb:P11607) physically interacts (MI:0915) with hδOR (uniprotkb:P41143) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7889430: SERCA2b (uniprotkb:P11607) physically interacts (MI:0915) with Calnexin (uniprotkb:P27824) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7889342: SERCA2b (uniprotkb:P16615) physically interacts (MI:0914) with Calnexin (uniprotkb:P27824) and hΔOR (uniprotkb:P41143) by anti bait coimmunoprecipitation (MI:0006)
MINT‐7889449: hδOR (uniprotkb:P41143) physically interacts (MI:0915) with Calnexin (uniprotkb:P27824) by anti tag coimmunoprecipitation (MI:0007)