bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular epidemiology and genetic evolution of avian influenza H5N1 subtype in Nigeria, 2006 to 2021
Authors: Adesola Ridwan O. , Onoja Bernard A. , Adamu Andrew M. , Agbaje Sheriff T. , Abdulazeez Modinat D. , Akinsulie Olalekan C. , Bakre Adetolase , Adegboye Oyelola A. 
Published: June 2024

AbstractNigeria recorded one of the earliest outbreaks of the Highly Pathogenic Avian Influenza (HPAI) virus H5N1 in 2006, which spread to other African countries. In 2023, 18 countries reported outbreaks of H5N1 in poultry, with human cases documented in Egypt, Nigeria, and Djibouti. There is limited information on the molecular epidemiology of HPAI H5N1 in Nigeria. We determined the molecular epidemiology and genetic evolution of the virus from 2006 to 2021. We investigated the trend and geographical distribution across Nigeria. The evolutionary history of 61 full-length genomes was performed from 13 countries worldwide, and compared with sequences obtained from the early outbreaks in Nigeria up to 2021. MEGA 11 was used to determine the phylogenetic relationships of H5N1 strains, which revealed close ancestry between sequences in Nigeria and those from other African countries. Clade classification was performed using the subspecies classification tool for Bacterial and Viral Bioinformatics Research Center (BV-BRC) version 3.35.5. H5N1 Clade 2.2 was observed in 2006, with 2.3.2, 2.3.2.1f clades observed afterwards and 2.3.4.4b in 2021. Our findings underscore the need for genomics surveillance to track antigenic variation and clades switching to monitor the epidemiological of the virus and safeguard human and animal health.Impacts

Specific variations in the hemagglutinin (HA) and neuraminidase (NA) genes of Avian influenza virus are consistent in different geographical regions.

H5N1 Clade 2.2 was reported in 2006, with 2.3.2, 2.3.2.1f afterwards and 2.3.4.4b in 2021.

Nigeria is an epicentre for avian influenza with three major migratory routes for wild birds transversing the country.

It is plausible that the Avian influenza in Northern Nigeria may be linked to wild bird sanctuaries in the region.
Sleep Disorders in Adults with Intellectual Disability: Current Status and Future Directions
Authors: Perera Bhathika , O’Regan David 
Published: June 2024

Abstract
Purpose of Review
We performed a narrative review of the current knowledge in epidemiology, mechanisms, clinical presentation, and treatment of sleep disorders in adults with intellectual disability.

Recent Findings
Adults with Intellectual disability face significant mental and physical health disparities, including in sleep medicine. Despite sleep disorders having been identified as adversely affecting the health and quality of life of both adults with intellectual disabilities and their caregivers, the evidence base for assessment, diagnosis and treatment in this population remains under developed when compared to that of the general population. This review summarises the existing literature on insomnia, sleep disordered breathing, and other common sleep disorders in adults with intellectual disability. Emphasising the existing gaps in our understanding of sleep disorders in adults with intellectual disabilities, we propose strategies to address and mitigate these gaps.

Summary
Sleep disorders are common, yet frequently ignored in adults with intellectual disabilities. To address sleep health inequality gaps, future research should adopt tailored approaches, considering the heterogeneity within the intellectual disability population, including stratification by aetiology and health comorbidities. This is essential to improve the diagnostic accuracy and treatment of sleep disorders in adults with intellectual disability.
Study of the Pectinase Production from Soursop and Cherimoya Pulp for Agro-Industrial Waste Reduction in Colombia
Authors: García Nora M. , Cely Nelcy M. , Méndez Paula A. 
Published: June 2024

AbstractPectic enzymes are an eco-friendly alternative to improve extraction processes for the food industry. The objective of the research was to obtain pectinase from the pulp of soursop (Annona muricata L.) and cherimoya (Annona cherimola L.), as an alternative to reduce agro-industrial waste in Colombia; involving the study of the factors influencing the protein content of the enzymes extracted from different stages and types of fruits, the conditions for enzymatic activity, and the potential application in the food industry, particularly in extraction processes and juice clarification. A factorial design 23, spectrometric and potentiometric techniques evidenced that the protein content depends on factors such as fruit stage (suitable “s” and unsuitable “u” fruits for consumption) and fruit type (soursop and cherimoya). The higher protein content was obtained by the precipitation method in comparison to ion exchange chromatography. Cherimoya fruit presented a higher protein content of 326 ± 26 mg by precipitation, however, the soursop samples evidenced higher enzymatic activity, 2.2 ± 0.1 U/mL. The pectinase obtained was an acidic enzyme of type pectin methylesterase with an enzymatic activity of 27.0 ± 0.9 U/mL, under the optimal conditions of pH 4 and temperature of 40 °C. The application in blackberry juice evidenced a decrease in the absorbance and pH changes. From the current research, the pulp of soursop and cherimoya was proven as a cheap source to obtain pectinase with enzymatic activity for juice clarification, and this process is an alternative to reduce the negative impact on the environment and an economical source for the local producers.
Asperuloside inhibits the activation of pancreatic cancer-associated fibroblasts via activating transcription factor 6
Authors: Cao Ling-zhi , Yang Fan-hui , Zhang Hao , Jia Ai-min , Li Su-ping , Wen Hu-ling 
Published: June 2024

Abstract
Background
Pancreatic cancer-associated fibroblasts (CAFs) play a crucial role in tumor progression and immune evasion. Asperuloside (ASP) is an iridoid glycoside with potential anti-tumor properties. This study aimed to explore the molecular mechanisms of ASP on CAFs, particularly focusing on its effects on activating transcription factor 6 (ATF6), a key regulator of endoplasmic reticulum stress.

Method
CAFs were treated with different concentrations of ASP (0, 1, 3, and 5 mM), and the role of ATF6 was investigated by over-expressing it in CAFs. Subsequently, western blot was used to detect ATF6, α-smooth muscle actin (α-SMA), fibroblast activating protein (FAP), and vimentin protein levels in CAFs. The collagen gel contraction assay and Transwell assay were applied to evaluate the contraction and migration ability of CAFs. In addition, the interleukin (IL)-6, C–C motif chemokine ligand (CCL)-2, and C-X-C motif chemokine ligand (CXCL)-10 levels were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

Results
CAFs had significantly higher expression levels of α-SMA, FAP, and vimentin compared to normal fibroblasts (NFs). ASP significantly inhibited the activation, contraction, and migration of CAFs in a concentration-dependent manner. ASP treatment also reduced the expression of cytokines (IL-6, CCL2, and CXCL10) and down-regulated ATF6 levels. Over-expression of ATF6 mitigated the inhibitory effects of ASP.

Conclusion
ASP exerts its anti-tumor effects by down-regulating ATF6, thereby inhibiting the activation and function of pancreatic CAFs. These findings suggest that ASP could be a promising therapeutic agent for pancreatic cancer by modulating the tumor microenvironment.
Challenges in non-destructive X-ray CT testing of riveted joints in the automotive industry
Authors: Schromm Thomas , Beckmann Felix , Moosmann Julian , Berthe Daniel , Pfeiffer Franz , Grosse Christian 
Published: June 2024

AbstractDespite recent advancements, contemporary laboratory-based industrial computed tomography (CT) technology has not yet achieved the status of an efficient and economically viable in situ non-destructive method for examining riveted joints that are commonly used in the automotive industry. 2D X-ray or 3D CT systems can only reliably assess a rivet joint’s condition in rare, and favorable circumstances. Moreover, a comprehensive analysis to identify the obstacles preventing CT from efficiently capturing high-resolution, and contrast-rich scans of riveted joints in general is lacking. This work delves into the challenges and limitations of the technology that prevent operators from reaching the desired precision required for rigorous quality control. The study specifically addresses questions concerning in situ reachability and positioning of the sample structure, radiodensity and beam hardening, the relationship between spot size and power, and economic feasibility—all within the context of analyzing riveted joints in the automotive industry. Additionally, we provide an abstracted overview of the current industrial X-ray tube market, which informs our discussion on these specific aspects. Through a combination of experimental findings, analytical insights, and deductions, we shed light on the persistent difficulties in achieving resolutions comparable to macro-sectioning and microscopy, which are currently considered the gold standard in examining riveted joints. Our investigation further explores the potential of a photon-counting detector paired with a conventional, laboratory-based X-ray source. Furthermore, we investigate the use of a synchrotron beamline as an X-ray source in conjunction with a custom-designed detector optimized for such beam geometries. This detector employs scintillators coupled with optical magnification, functioning similarly to an X-ray microscope. Our findings and data collection conclusively reveal that commercially available CT systems still fail to meet the requirements for efficient testing, underscoring the urgent need for further research and development to enable widespread implementation.
Assessing the determinants of saving behaviour: evidence from rural farming households in Central Uganda
Authors: Midamba Dick Chune , Jjengo Accram , Ouko Kevin Okoth 
Published: June 2024

AbstractSavings play a significant role in any country’s economic development. Notably, because farmers tend to have seasonal income from their farming activities, they also tend to be highly vulnerable to poor saving habbit than other occupations, such as those in formal jobs. However, farmers who save part of their income for subsequent production can purchase farm inputs in time as they wait for the onset of rain. Reportedly, there has been poor saving behavior among farmers in sub-Saharan Africa. Therefore, this study aims to determine the factors responsible for farmers’ saving behavior. Descriptive and econometric (binary logistic model) analyses were employed to achieve the objectives of the study. The results indicate that the majority of farmers saved on a monthly and weekly basis. The results of the binary logistic regression model analysis showed that age, marital status, gender, experience, group membership, distance to the markets and markets, farm income, and farmers’ sub-counties of residence had a significant influence on farmers’ saving behavior. From the results, policy measures to increase the rate of savings include the employment of more extension personnel to reach as many farmers as possible. Government and extension agents should target female and less experienced farmers through adult-based education programs because they are vulnerable to poor saving behavior. Farmers should join farmer—based groups and cooperative societies, in which saving information is disseminated. The government, non-governmental organizations and financial institutions should offer financial literacy training on savings to smallholder farmers.
Video Game Addiction and its Relationship with Sleep Quality among Medical Students
Authors: Alghamdi Faisal Ahmed Dakheel-allah , Alghamdi Faisal Ahmed Ghanim , Abusulaiman Abdullah , Alsulami Abdulaziz Jabr , Bamotref Mohammed , Alosaimi Albraa , Bamousa Omar , Wali Siraj Omar 
Published: June 2024

Abstract
Background
Although many health problems, including sleep disorders, have been associated with video gaming, further studies are required to establish the validity of these connections. This study aimed to determine the prevalence of gaming addiction among medical students and its association with poor sleep quality, which may be reflected in academic performance.

Method
A cross-sectional survey was conducted between January and June 2023 among medical students at the institution under study. An online survey was conducted and was divided into three sections. The first section included the demographic data, the second section included the 7-item Gaming Addiction Scale (GAS), and the third section included the Pittsburgh Sleep Quality Index. Using the GAS, and based on the total score, gamers were classified as addicted, problematic, engaged, or normal. Hence, abnormal gamers include engaged, problematic, and addicted gamers.

Result
There were 356 participants with a mean age of 22.5 -/+ 1.8 years, and 75.3% were males. The data showed that 38.8% of the study population were abnormal gamers: 40 (11.2%) engaged gamers, 81 (22.8%) problematic gamers, and 17 (4.8%) addicted gamers. Furthermore, abnormal gaming was linked to poor sleep quality when comparing abnormal gamers with normal gamers (92% vs. 80.3%, p = 0.002). Further comparison between the types of abnormal gamers revealed that addicted gamers were found to rely on sleep medication to help them sleep at night and took longer time to fall asleep (p = 0.050 and p = 0.045, respectively).

Conclusion
Abnormal gamers are common among medical students and strongly associated with poor sleep quality compared to normal gamers.
Bilateral tensor fascia suralis muscles and unilateral accessory biceps femoris muscle: a cadaveric case study
Authors: Swancutt Makayla M. , Hailey Jared M. , Dennis Jennifer F. 
Published: June 2024

Abstract
Purpose
This study reports the findings of bilateral tensor fascia suralis muscles (TFS) and a unilateral accessory muscle belly of the biceps femoris (AMBF) in a 72-year-old female.

Case report
The findings were initially identified during educational dissection of the lower extremities by medical students. The AMBF and bilateral TFS were further dissected to identify their origins and insertions. The left TFS (length, 22.83 ± 0.05 cm; width, 6.24 ± 0.14 mm) originated from the biceps femoris long head muscle (BFLH) and inserted onto the crural fascia overlying the lateral head of gastrocnemius muscle. The right TFS (length, 114.89 ± 0.62 mm; width, 9.82 ± 0.11 mm) originated from the BFLH and inserted onto the crural fascia overlying the medial head of gastrocnemius muscle. The length of the crural fascia insertion was 76.26 ± 3.00 mm (left) and 127.69 ± 1.41 mm (right). An AMBF was present in the left lower limb, originating from the greater trochanter and inserting on the distal end of the BFLH. The common peroneal nerve passed deep to the AMBF, 20.3 cm inferior to the inferior border of the piriformis.

Conclusion
This data characterizes a novel finding of bilateral TFS muscles with a unilateral AMBF.
Indicators for the Assessment of the Impact of Hydropeaking on Aquifers
Authors: Basilio Hazas M. , Marcolini G. , Wohlmuth B. , Chiogna G. 
Published: June 2024

AbstractThe operation of hydropower plants leads to sudden changes in river stage and to a flow regime known as hydropeaking. Hydropeaking alters the morphology of the riverbed and water quality, and ultimately poses a risk to riverine ecosystems. While many indicators are available to quantitatively assess this problem in rivers, the impact of hydropeaking on aquifers is largely unknown and lacks of quantitative indicators. We analyze with wavelet techniques the spatial and temporal dynamics of surface water‐groundwater interaction in an aquifer impacted by two differently regulated rivers. We propose four indicators to study the aquifer stress produced by hydropeaking and classify the observed groundwater head time series into weakly, moderately and highly impacted. This study opens the possibility for a quantitative assessment of the impact of hydropeaking on the groundwater ecosystem.
Shallow Slow Slip Events in the Imperial Valley With Along‐Strike Propagation
Authors: Materna Kathryn , Bürgmann Roland , Lindsay Danielle , Bilham Roger , Herring Thomas , Crowell Brendan , Szeliga Walter 
Published: June 2024

AbstractShallow creep events provide opportunities to understand the mechanical properties and behavior of faults. However, due to physical limitations observing creep events, the precise spatio‐temporal evolution of slip during creep events is not well understood. In 2023, the Superstition Hills and Imperial faults in California each experienced centimeter‐scale slip events that were captured in unprecedented detail by satellite radar, sub‐daily Global Navigation Satellite Systems, and creepmeters. In both cases, the slip propagated along the fault over 2–3 weeks. The Superstition Hills event propagated bilaterally away from its initiation point at average velocities of ∼9 km/day, but propagation velocities were locally much higher. The ruptures were consistent with slip from tens of meters to ∼2 km depths. These slowly propagating events reveal that the shallow crust of the Imperial Valley does not obey purely velocity‐strengthening or velocity‐weakening rate‐and‐state friction, but instead requires the consideration of fault heterogeneity or fault‐frictional behaviors such as dilatant strengthening.
The Age of Buried Carbon Changes the Greenhouse Gas Budget of a Dam
Authors: Tittel J. , Rosenlöcher Y. , Dadi T. , Lechtenfeld O. J. , Simon C. 
Published: June 2024

AbstractDams are a globally relevant source of greenhouse gases (GHG), which impairs their function as a source of green energy. High burial rates of organic carbon (OC) in dam sediments may partly or fully offset the emissions. We argue that only the burial of carbon fixed in the timespan of dam operation changes the GHG balance. Here, we took sediment cores from a temperate dam. We analyzed radiocarbon age and OC molecular composition by laser desorption ionization mass spectrometry in the bulk OC and in four chemical extract fractions. The bulk samples contained modern OC, fixed after 1950. However, the extracted OC was of different ages (modern to 1900 years BP). Fractions with OC measured as old (>960 years BP) accounted for 57% of total sediment OC. Correlations of molecular composition with extract age suggest that these older fractions contained insignificant amounts of modern OC. We conclude that a substantial proportion of buried carbon did not originate from the contemporary atmosphere and cannot be offset against recent GHG emissions.
Long‐Term Monitoring of the Annual Irrigated Cropland Extent in Fragmented and Heterogeneous Arid Landscapes Using Machine Learning and Landsat Imagery
Authors: Tan Meibao , Ran Youhua , Feng Min , Dong Guotao , Du Deyan , Zhu Gaofeng , Nian Yanyun , Li Xin 
Published: June 2024

AbstractUnderstanding the long‐term spatiotemporal evolution of irrigated cropland is essential for water resource management, but this knowledge remains elusive in most water‐stressed arid areas. In this study, we introduced an integrated framework for long‐term and field‐scale mapping of annual irrigated cropland in arid and semiarid regions. This framework combines the k‐means algorithm with a semiautomatically trained random forest classifier for initial classification and employs the Bayesian Updating of Land Cover algorithm for subsequent postprocessing. Taking the Heihe River basin in northwestern China as the experimental area, we generated 30‐m annual irrigated cropland maps spanning from 1990 to 2020 based on Landsat imagery and the Google Earth Engine. Comprehensive validation confirmed the reliability of this approach, with the overall accuracy of the annual maps ranging from 83% to 88.3% (mean: 86.6%). Our data set provides an unprecedentedly long‐term and fine‐scale perspective for understanding the continuous spatial and temporal dynamics of irrigated cropland in the Heihe River basin, surpassing previous studies in Central Asia and northwestern China. Notably, a rapid expansion of irrigated areas is occurring in the basin, especially in the water‐stressed midstream and downstream areas. This finding points to potential ecological risks in the foreseeable future due to water resource constraints.
Electrical Conductivity of Dense MgSiO3 Melt Under Static Compression
Authors: Okuda Yoshiyuki , Hirose Kei , Ohta Kenji , Kawaguchi‐Imada Saori , Oka Kenta 
Published: June 2024

AbstractThe magnetic fields of terrestrial planets are created by core convection. Molten silicate mantles could also generate magnetic fields through their convective motion, known as a silicate dynamo. Recent computational studies have suggested that silicate melts may exhibit high electrical conductivity (EC) at temperatures above 4000 K due to strong electronic conduction, which could activate a silicate dynamo. We determined the EC of dense molten MgSiO3 up to 71 GPa and 4490 K by static compression experiments. It jumped by one order of magnitude upon melting, but 57(27) S/m at 4490 K is much lower than previous predictions, suggesting that molten MgSiO3 carries charge via ions rather than predicted electronic conduction. Nevertheless, the strong temperature dependence of the ionic conductivity found in this study suggests that super‐Earths’ hotter magma ocean with larger‐scale convection could power a dynamo that drives magnetic fields, which plays key roles in sustaining planetary surface environments.
Krause corpuscles are genital vibrotactile sensors for sexual behaviours
Authors: Qi Lijun , Iskols Michael , Greenberg Rachel S. , Xiao Jia Yin , Handler Annie , Liberles Stephen D. , Ginty David D. 
Published: June 2024

AbstractKrause corpuscles, which were discovered in the 1850s, are specialized sensory structures found within the genitalia and other mucocutaneous tissues1–4. The physiological properties and functions of Krause corpuscles have remained unclear since their discovery. Here we report the anatomical and physiological properties of Krause corpuscles of the mouse clitoris and penis and their roles in sexual behaviour. We observed a high density of Krause corpuscles in the clitoris compared with the penis. Using mouse genetic tools, we identified two distinct somatosensory neuron subtypes that innervate Krause corpuscles of both the clitoris and penis and project to a unique sensory terminal region of the spinal cord. In vivo electrophysiology and calcium imaging experiments showed that both Krause corpuscle afferent types are A-fibre rapid-adapting low-threshold mechanoreceptors, optimally tuned to dynamic, light-touch and mechanical vibrations (40–80 Hz) applied to the clitoris or penis. Functionally, selective optogenetic activation of Krause corpuscle afferent terminals evoked penile erection in male mice and vaginal contraction in female mice, while genetic ablation of Krause corpuscles impaired intromission and ejaculation of males and reduced sexual receptivity of females. Thus, Krause corpuscles of the clitoris and penis are highly sensitive mechanical vibration detectors that mediate sexually dimorphic mating behaviours.