bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluación imagenológica de los cambios morfológicos en la articulación temporomandibular de pacientes en crecimiento tratados con aparatos funcionales. Revisión sistemática
Authors: Tomàs Aliberas Jordi , Pizarro Lavin Catalina , Sotorra Figuerola Dídac , Felipe Spada Natalia 
Published: June 2024

Antecedentes: Una amplia gama de aparatos funcionales permiten estimular el crecimiento mandibular de pacientes clase II, mediante su avance mandibular; logrando corregir este tipo de desarmonía esquelética y oclusal. Los aparatos de reposicionamiento fijos aplican fuerzas constantes sobre la ATM y pueden causar la remodelación del cóndilo articular y de la fosa glenoidea. La tomografía computarizada (TC) ha sido el estándar de oro para obtener imágenes del hueso y tejidos duros de la ATM, pero hoy en día se emplean la tomografía computarizada de haz cónico (TCHC) y la Resonancia Magnética Nuclear (RMN), a causa de sus niveles bajos y nulos respectivamente, de radiación. Objetivo: medir los cambios morfológicos mediante evaluación imagenológica de la ATM después de la corrección de la maloclusión de clase II con tratamiento funcional con aparatos en pacientes en crecimiento Métodos: se realizó una búsqueda bibliográfica sistemática integral desde enero de 2015 hasta mayo de 2023 en las siguientes bases de datos bibliográficas PubMed, EBSCO Embase y la Biblioteca Cochrane. Resultados: 10 estudios fueron seleccionados. El tamaño muestral total fue de 210 pacientes, y el rango de edad fue de 10,3 a 14 años. El principal método de imagen utilizado fue la TCHC con n = 9. Conclusiones: El principal cambio descrito es el remodelado condilar, seguido de la adaptación morfológica de la fosa. Aunque no se han reportado diferencias significativas en los cambios morfológicos en la ATM entre aparatos funcionales fijos versus removibles, la aparatología fija causa mayor incidencia de cambios morfológicos.
Reliability Analysis for Rotate Vector Reducer by Combining Polynomial Chaos Expansion and Saddlepoint Approximation Considering Multi-Failure Modes
Authors: Yang Shunqi , Xiao Huipeng , Lu Pan , Xu Guohua , Li Hao , Zhang Xiaoling 
Published: June 2024

Abstract
Rotate vector (RV) reducer is an essential mechanical transmission device in industrial machinery, robotics, aerospace, and other fields. The dynamic transmission characteristics and strength of the cycloidal pin gear and turning-arm bearing significantly affect the motion accuracy and reliability of RV reducer. Uncertainties from manufacturing and assembly errors and working loads add complexity to these effects. Developing effective methods for uncertainty propagation and reliability analysis for the RV reducer is crucial. In this work, the mail failure modes of RV reducer are studied, and an effective reliability analysis method for RV reducer considering the correlation between multi-failure modes is proposed by combining polynomial chaos expansion (PCE) and saddlepoint approximation method (SPA). This paper develops an uncertainty propagation strategy for RV reducer based on dynamic simulation and PCE method with high accuracy. On this basis, a surrogated cumulant generating function (CGF) and SPA are combined to analyze the stochastic characteristics of the failure behaviors. Then, based on the probability density function (PDF) and cumulative distribution function (CDF) calculated by SPA, the copula function is employed to quantify the correlations between the multi-failure modes. Further, the system reliability with multi-failure modes is estimated by SPA and optimal copula function. The validity of the proposed approach is illustrated by RV-320E reducer reliability estimation, and the results show that the proposed method can provide an effective reliability assessment technology for complex system under unknown physical model and distribution characteristics.
Overcoming analytical and preanalytical challenges associated with extragenital home collected STI specimens
Authors: Hockman B. E. , Qi M. , Rotblatt H. , Borenstein L. , Flynn R. A. , Muldrow R. A. , Rajagopalan S. , Greene D. N. 
Published: June 2024

ABSTRACT

Home sample collection for sexually transmitted infection (STI) screening options can improve access to sexual healthcare across communities. For
Chlamydia trachomatis
(CT) and
Neisseria gonorrhoeae
(NG), genital infections have classically been the focus for remote collection options. However, infections may go undiagnosed if sampling is limited to urogenital sites because some individuals only participate in oral and/or anal intercourse. Here we evaluated samples for CT/NG detection after several pre-analytical collection challenges. A paired provider to self-collection validation was performed on rectal [
n
= 162; 22 + for CT and 9 + for NG by provider-collected (PC)] and throat (
N
= 158; 2 + for CT and 11 + for NG by provider-collected) swabs. The positive percent agreement for CT and NG ranged from 90.9% to 100%. The discrepancies were more often positive on self-collected (SC) (
n
= 9 SC+/PC−;
n
= 1 PC+/SC−;
n
= 1 PC+/SC Equiv.;
n
= 2 PC−/SC Equiv.). An empirical limit of detection (LoD) lower than the manufacturer’s claim (0.031 vs 2.5 IFU/mL for CT and 0.063 vs 124.8 CFU/ml for NG, respectively) was used to challenge additional variables. Common hand contaminants, including soap, hand sanitizer, lotion, and sunscreen were added to known positive (3× empirical LoD) or negative samples and did not influence detection. Samples at 2× and 10× the empirical LoD were challenged with extreme temperature cycling and extended room temperature storage. Detection was not affected by these conditions. These results indicate that remote self-collection is an appropriate method of sample acquisition for detecting extragenital CT/NG infections. Additionally, they provide a foundation towards meeting the regulatory standards for commercial testing of home collected extragenital samples.

IMPORTANCE
There is a clinical need for expanded extragenital bacterial sexually transmitted infection (STI) testing options, but the current regulatory landscape limits the wide-spread promotion and adoption of such services. Improved access, particularly for the LGBTQ+ community, can be achieved by validating testing for specimens that are self-collected at a remote location and arrive at the laboratory via a postal carrier or other intermediary route. Here we provide valuable data showing that self-collected samples for anal and oropharyngeal STI testing are equally or increasingly sensitive compared with those collected by a provider. We systematically consider the effects of storage time, exposure to temperature extremes, and the addition of common toiletries on results.
On geodesics in spherical Rindler space
Authors: Culetu Hristu 
Published: June 2024

The geodesics in various spherical Rindler frames are investigated. A display of some kinematical quantities of the spacetime is given. The constant acceleration from the metric acts as the surface gravity of the horizon [Formula: see text]. The radial geodesics are computed both for the Balasubramanian et al. form of the spherical Rindler space and for the non-diagonal metric of Huang and Sun.
Unraveling the Nexus Between Financial Openness and Environmental Quality: Green Finance as the Catalyst in CEE Countries
Authors: Dilanchiev Azer , Somthawinpongsai Chanyanan , Urinov Bobur , Eyvazov Elchin 
Published: June 2024

The use of fossil fuels, which is still seen as the cheapest energy source today, has increased carbon emissions and contributed to the formation of greenhouse gases, and this gas has reached levels that threaten world health. While studies on this problem often question the relationship between production volume, energy use, and carbon emissions, few studies question the effects of the financial assets, which are the providers of investments made for production purposes, on environmental degradation. This study used the fixed effect model and system GMM technique to analyse the moderating effect of green finance in connection to financial openness and environmental quality from 2015 to 2020 in the CEE countries. Findings show, among other things that (1) CO2 in CEE countries is still growing; (2) financial openness is a positive and significant indicator of CO2 emission; and (3) that green finance usage reduces the positive impact of financial openness on CO2 emissions. The study suggests that financial openness, renewable energy (RE) investment, and green technology innovation (GTI)– consistently drive CO2 emissions in CEE countries. The study suggests that policies promoting green finance, particularly RE investment and GTI, can help mitigate environmental degradation and reduce CO2 emissions.
An Analysis of PIK3CA Hotspot Mutations and Response to Neoadjuvant Therapy in Patients with Breast Cancer from Four Prospective Clinical Trials
Authors: Jank Paul , Karn Thomas , van Mackelenbergh Marion , Lindner Judith , Treue Denise , Huober Jens , Engels Knut , Solbach Christine , Diebold Kurt , Marmé Frederik , Müller Volkmar , Schneeweiss Andreas , Sinn Hans-Peter , Fehm Tanja , Schem Christian , Stickeler Elmar , Fasching Peter , Budczies Jan , Felder Bärbel , Nekljudova Valentina , Holtschmidt Johannes , Untch Michael , Denkert Carsten , Loibl Sibylle 
Published: June 2024

Abstract

Purpose:
The PI3K signaling pathway is frequently dysregulated in breast cancer, and mutations in PIK3CA are relevant for therapy resistance in HER2-positive (HER2pos) breast cancer. Mutations in exons 9 or 20 may have different impacts on response to neoadjuvant chemotherapy-based treatment regimens.

Experimental Design:
We investigated PIK3CA mutations in 1,691 patients with early breast cancer who were randomized into four neoadjuvant multicenter trials: GeparQuattro (NCT00288002), GeparQuinto (NCT00567554), GeparSixto (NCT01426880), and GeparSepto (NCT01583426). The role of different PIK3CA exons and hotspots for pathologic complete response (pCR) following neoadjuvant chemotherapy (NACT) and patient survival were evaluated for distinct molecular subgroups and anti-HER2 treatment procedures.

Results:
A total of 302 patients (17.9%) of the full cohort of 1,691 patients had a tumor with a PIK3CA mutation, with a different prevalence in molecular subgroups: luminal/HER2-negative (HER2neg) 95 of 404 (23.5%), HER2pos 170 of 819 (20.8%), and triple-negative breast cancer 37 of 468 patients (7.9%). We identified the mutations in PIK3CA exon 20 to be linked with worse response to anti-HER2 treatment (OR = 0.507; 95% confidence interval, 0.320–0.802; P = 0.004), especially in hormone receptor–positive HER2-positive breast cancer (OR = 0.445; 95% confidence interval, 0.237–0.837; P = 0.012). In contrast, exon 9 hotspot mutations p.E452K and p.E545K revealed no noteworthy differences in response therapy. Luminal/HER2neg patients show a trend to have worse treatment response when PIK3CA was mutated. Interestingly, patients with residual disease following neoadjuvant treatment had better survival rates when PIK3CA was mutated.

Conclusions:
The PIK3CA hotspot mutation p.H1047R is associated with worse pCR rates following NACT in HER2pos breast cancer, whereas hotspot mutations in exon 9 seem to have less impact.
MRI free water mediates the association between water exchange rate across the blood brain barrier and executive function among older adults
Authors: Pappas Colleen , Bauer Christopher E. , Zachariou Valentinos , Maillard Pauline , Caprihan Arvind , Shao Xingfeng , Wang Danny J.J. , Gold Brian T. 
Published: June 2024

Abstract
Vascular risk factors contribute to cognitive aging, with one such risk factor being dysfunction of the blood brain barrier (BBB). Studies using non-invasive magnetic resonance imaging (MRI) techniques, such as diffusion prepared arterial spin labeling (DP-ASL), can estimate BBB function by measuring water exchange rate (kw). DP-ASL kw has been associated with cognition, but the directionality and strength of the relationship is still under investigation. An additional variable that measures water in extracellular space and impacts cognition, MRI free water (FW), may help explain prior findings. A total of 94 older adults without dementia (Mean age = 74.17 years, 59.6% female) underwent MRI (DP-ASL, diffusion weighted imaging (DWI)) and cognitive assessment. Mean kw was computed across the whole brain (WB), and mean white matter FW was computed across all white matter. The relationship between kw and three cognitive domains (executive function, processing speed, memory) was tested using multiple linear regression. FW was tested as a mediator of the kw-cognitive relationship using the PROCESS macro. A positive association was found between WB kw and executive function [F(4,85) = 7.81, p &lt; .001, R2= 0.269; β = .245, p = .014]. Further, this effect was qualified by subsequent results showing that FW was a mediator of the WB kw-executive function relationship (indirect effect results: standardized effect = .060, bootstrap confidence interval = .0006 to .1411). Results suggest that lower water exchange rate (kw) may contribute to greater total white matter (WM) FW which, in turn, may disrupt executive function. Taken together, proper fluid clearance at the BBB contributes to higher-order cognitive abilities.
Comparative study on ionic dissolution and structural changes of montmorillonite, kaolinite and muscovite during interfacial reactions with oxalic acid solution
Authors: Zeng Li , Peng Tongjiang , Sun Hongjuan , Zhang Xiyue , Zhao Dingran 
Published: June 2024

AbstractOrganic acids are commonly found in soils and sediments, playing an important role in the alteration and weathering of minerals and influencing a series of geochemical processes such as soil fertility, metal cycling and pollutant migration. In order to better comprehend the reaction mechanisms of different layered silicate minerals with organic acids, three minerals with various structure types, namely montmorillonite, kaolinite and muscovite, were investigated in this work. In particular, the effects of interfacial reactions with oxalic acid on the crystal structure, chemical composition, morphology and specific surface area of minerals were compared. The composition and structure of montmorillonite, kaolinite and muscovite during the interfacial reaction with oxalic acid were characterised using powder X-ray diffraction (XRD), scanning electron microscopy (SEM) and inductively coupled plasma-optical emission spectroscopy (ICP-OES) methods. It was shown that Si4+ and Al3+ were dissolved gradually during the interfacial reactions and that the changes in the properties of minerals depended on structural characteristics. After 300 days of the interfacial reactions with oxalic acid, the dissolution percentages of Si4+ and Al3+ in montmorillonite, kaolinite and muscovite were 12.7%, 8.4%, 3.8% and 62.1%, 30.7%, 6.1%, respectively. Moreover, the lamellar morphology of montmorillonite was destroyed upon the interfacial reaction with oxalic acid, and irregular particles with sizes of ~100–500 nm were formed on the surface. The diameter of kaolinite flake particles decreased from 400–1500 nm to 50–400 nm, and the surface of rod-shaped particles was ruptured. The small particles disappeared from the muscovite surface, and the initially sharp edges became blunted. The specific surface area and the total pore volume of montmorillonite and kaolinite increased after the interfacial reaction with oxalic acid, whereas the opposite results were obtained for muscovite. The differential dissolution of the minerals during their interfacial reaction with oxalic acid was mainly related to the differences between cation occupancies, structural types, chemical bond strengths and specific surface areas.
bsgenova: an accurate, robust, and fast genotype caller for bisulfite-sequencing data
Authors: Feng Yance , Gao Fei 
Published: June 2024

Abstract
Background
Bisulfite sequencing (BS-Seq) is a fundamental technique for characterizing DNA methylation profiles. Genotype calling from bisulfite-converted BS-Seq data allows allele-specific methylation analysis and the concurrent exploration of genetic and epigenetic profiles. Despite various methods have been proposed, single nucleotide polymorphisms (SNPs) calling from BS-Seq data, particularly for SNPs on chromosome X and in the presence of contaminative data, poses ongoing challenges.

Results
We introduce bsgenova, a novel SNP caller tailored for bisulfite sequencing data, employing a Bayesian multinomial model. The performance of bsgenova is assessed by comparing SNPs called from real-world BS-Seq data with those from corresponding whole-genome sequencing (WGS) data across three human cell lines. bsgenova is both sensitive and precise, especially for chromosome X, compared with three existing methods. Moreover, in the presence of low-quality reads, bsgenova outperforms other methods notably. In addition, bsgenova is meticulously implemented, leveraging matrix imputation and multi-process parallelization. Compared to existing methods, bsgenova stands out for its speed and efficiency in memory and disk usage. Furthermore, bsgenova integrates bsextractor, a methylation extractor, enhancing its flexibility and expanding its utility.

Conclusions
We introduce bsgenova for SNP calling from bisulfite-sequencing data. The source code is available at https://github.com/hippo-yf/bsgenova under license GPL-3.0.
Identification and analysis of short-term and long-term salt-associated lncRNAs in the leaf of Avicennia marina
Authors: Wang Lingling , Fu Yixuan , Yuan Zixin , Wang Jingyi , Guan Yali 
Published: June 2024

AbstractAs a highly salt-resistant mangrove, Avicennia marina can thrive in the hypersaline water. The leaves of Avicennia marina play a crucial role in salinity stress adaptability by secreting salt. Although the functions of long non-coding RNAs (lncRNAs) in leaves remain unknown, they have emerged as regulators in leaf development, aging and salt response. In this study, we employed transcriptomic data of both short-term and long-term salt treated leaves to identify salt-associated lncRNAs of leaf tissue. As a result, 687 short-term and 797 long-term salt-associated lncRNAs were identified. Notably, both short-term and long-term salt-associated lncRNAs exhibited slightly longer lengths and larger exons, but smaller introns compared with salt-non-associated lncRNAs. Furthermore, salt-associated lncRNAs also displayed higher tissue-specificity than salt-non-associated lncRNAs. Most of the salt-associated lncRNAs were common to short- and long-term salt treatments. And about one fifth of the downregulated salt-associated lncRNAs identified both in two terms were leaf tissue-specific lncRNAs. Besides, these leaf-specific lncRNAs were found to be involved in the oxidation–reduction and photosynthesis processes, as well as several metabolic processes, suggesting the noticeable functions of salt-associated lncRNAs in regulating salt responses of Avicennia marina leaves.
Germination responses of Lens Culiunaris L. seeds to osmotic potentials at cardinal temperatures using hydrothermal time model
Authors: Ullah Ibrar , Ullah Sami , Amin Fazal , Al-Hawadi Jehad S. , Okla Mohammad K. , Alaraidh Ibrahim A. , AbdElgawad Hamada , Liu Ke , Harrison Matthew Tom , Saud Shah , Hassan Shah , Nawaz Taufiq , Zhu Mo , Liu Haitao , Fahad Shah 
Published: June 2024

Abstract
Background
Lentil is a significant legume that are consumed as a staple food and have a significant economic impact around the world. The purpose of the present research on lentil was to assess the hydrothermal time model’s capacity to explain the dynamics of Lens culinaris L. var. Markaz-09 seed germination, as well as to ascertain the germination responses at various sub-optimal temperatures (T) and water potentials (Ψ). In order to study lentil seed germination (SG) behavior at variable water potentials (Ψs) and temperatures (Ts). A lab experiment employing the hydrothermal time model was created. Seeds were germinated at six distinct temperatures: 15 0С, 20 0С, 25 0С, 30 0С, 35 0С, and 40 0С, with five Ψs of 0, -0.3, -0.6, -0.9, and − 1.2 MPa in a PEG-6000 (Polyethylene glycol 6000) solution.

Results
The results indicated that the agronomic parameters like Germination index (GI), Germination energy (GE), Timson germination index (TGI), were maximum in 25 0C at (-0.9 MPa) and lowest at 40 0C in 0 MPa. On other hand, mean germination time (MGT) value was highest at 15 0C in -1.2 MPa and minimum at 40 0C in (-0.6 MPa) while Mean germination rate (MGR) was maximum at 40 0C in (0 MPa) and minimum at 15 0C in (-0.6 MPa).

Conclusions
The HTT model eventually defined the germination response of Lens culinaris L. var. Markaz-09 (Lentil) for all Ts and Ψs, allowing it to be employed as a predictive tool in Lens culinaris L. var. Markaz-09 (Lentil) seed germination simulation models.
High SARS-CoV-2 infection rate in children unvaccinated with COVID-19 vaccine in Changzhou, China, shortly after lifting zero-COVID-19 policy in December 2022
Authors: Tang Jie , Wang Yong , Lu Weiqin , Gao Zhihong , Xu Mingfeng , Wu Lin , Jin Jianhua 
Published: June 2024

Abstract
Background
China experienced an overwhelming COVID-19 pandemic from middle December 2022 to middle January 2023 after lifting the zero-COVID-19 policy on December 7, 2022. However, the infection rate was less studied. We aimed to investigate the SARS-CoV-2 infection rate in children shortly after discontinuation of the zero-COVID-19 policy.

Methods
From February 20 to April 10, 2023, we included 393 children aged 8 months to less than 3 years who did not receive COVID-19 vaccination and 114 children aged 3 to 6 years who received inactivated COVID-19 vaccines based on the convenience sampling in this cross-sectional study. IgG and IgM antibodies against nucleocapsid (N) and subunit 1 of spike (S1) of SARS-CoV-2 (anti-N/S1) were measured with commercial kits (Shenzhen YHLO Biotech, China).

Results
Of the 393 unvaccinated children (1.5 ± 0.6 years; 52.2% boys), 369 (93.9%) were anti-N/S1 IgG positive. Of the 114 vaccinated children (5.3 ± 0.9 years; 48.2% boys), 112 (98.2%) were anti-N/S1 IgG positive. None of the unvaccinated or vaccinated children was anti-N/S1 IgM positive. The median IgG antibody titers in vaccinated children (344.91 AU/mL) were significantly higher than that in unvaccinated children (42.80 AU/mL) (P < 0.0001). The positive rates and titers of anti-N/S1 IgG had no significant difference between boys and girls respectively.

Conclusion
Vast majority of children were infected with SARS-CoV-2 shortly after ending zero-COVID-19 policy in China. Whether these unvaccinated infected children should receive COVID-19 vaccine merits further investigation.