bluuebunny/crossref_metadata_2025_split huggingface dataset:
The problematic art of conversation: Communication and health practice evolution
Authors: Walsh Kenneth , Jordan Zoe , Apolloni Lucia 
Published: July 2009

AbstractConversation is an important part of everyday life and is increasingly recognized as being critical to the success of practice change initiatives in the health care environment. However, conversation is becoming something of a lost art and it is important that we consciously ‘create the space’ to have constructive, meaningful and inclusive conversations in and around health practices.This article explores the importance of conversation to organizational functioning, and the potential for practice development to facilitate meaningful conversations around clinical practice and practice change. It also discusses a form of conversation called future‐focused conversation as one approach to assist cultural evolution in health environments. Copyright © 2009 John Wiley & Sons, Ltd.
Non‐aspirin NSAIDs, cyclooxygenase‐2 inhibitors and risk for cardiovascular events–stroke, acute myocardial infarction, and death from coronary heart disease
Authors: Roumie Christianne L. , Choma Neesha N. , Kaltenbach Lisa , Mitchel, Jr Edward F. , Arbogast Patrick G. , Griffin Marie R. 
Published: July 2009

AbstractPurposeTo determine if certain non‐steroidal anti‐inflammatory drugs (NSAIDs) are associated with increased risk of cardiovascular events: acute myocardial infarction (AMI), stroke, and death from coronary heart disease (CHD).MethodsWe conducted a retrospective cohort study of Tennessee Medicaid enrollees aged 35–94 years between 1 January 1999 and 31 December 2005. Eligible persons were non‐institutionalized, had continuous enrollment, and had no serious illness prior to cohort entry. Exposure to celecoxib, rofecoxib, valdecoxib, ibuprofen, naproxen, diclofenac, and indomethacin was studied. The outcome was hospitalization for AMI, stroke, or death from CHD among those with and without a history of cardiovascular disease (CVD). Adjusted hazard ratios (aHR) and 95% confidence intervals (95% CI) are reported.ResultsThere were 610 001 persons in the final cohort and 14% had a baseline history of CVD. In those without CVD (N = 525 249) there were 1 566 678 person‐years of follow‐up and 12 184 events. In this group, non‐users had 7.90 events/1000 person‐years. Events/1000 person‐years were 10.41 for current use of celecoxib (aHR 1.00, 95% CI 0.89–1.13), 10.91 for rofecoxib (aHR 1.21, 95% CI 1.07–1.37), 12.46 for valdecoxib (aHR 1.30 95% CI 1.04–1.61), and 13.25 for indomethacin (aHR 1.36, 95% CI 1.11–1.66) compared to non‐users. Among patients with a past history of CVD (N = 84 752) there were 397 977 person‐years of follow‐up and 10 248 events. Non‐users had 28.30 events/1000 person‐years. Among those with CVD, rofecoxib use was associated with increased event rate (30.28 events/1000 person‐years [aHR 1.21, 95% CI 1.08–1.37]) and naproxen was associated with a decreased event rate (22.66 events/1000 person‐years [aHR 0.88, 95% CI 0.79–0.99]). Among new users, the results were similar except risk among naproxen users was no longer different than non‐users.ConclusionsWe found an increased risk of cardiovascular events among all and new current users of rofecoxib, valdecoxib, and indomethacin in patients with no history of CVD. Among patients with CVD, all and new current rofecoxib use was associated with an increased risk of a cardiovascular event. Copyright © 2009 John Wiley & Sons, Ltd.
Proteomic profiling of the prechylomicron transport vesicle involved in the assembly and secretion of apoB‐48‐containing chylomicrons in the intestinal enterocytes
Authors: Wong Diana M. , Webb Jennifer P. , Malinowski Paul M. , Macri Joseph , Adeli Khosrow 
Published: July 2009

AbstractIntracellular assembly of chylomicrons (CM) occurs in intestinal enterocytes through a series of complex vesicular interactions. CM are transported from the ER to the Golgi using a specialized vesicular compartment called the prechylomicron transport vesicle (PCTV). In this study, PCTVs were isolated from the enteric ER of the Syrian Golden hamster, and characterized using 2‐DE and MS. Proteomic profiles of PCTV‐associated proteins were developed with the intention of identifying proteins involved in the formation, transport, lipidation, and assembly of CM particles. Positively identified proteins included those involved in lipoprotein assembly, namely microsomal triglyceride transfer protein and apolipoprotein B‐48, as well as proteins involved in vesicular transport, such as Sar1 and vesicle‐associated membrane protein 7. Other groups of proteins found were chaperones, intracellular vesicular trafficking proteins, fatty acid‐binding proteins, and lipid‐related proteins. These findings have increased our understanding of the transport vesicle involved in the intracellular assembly and transport of CM and can provide insight into potential cellular factors responsible for dysregulation of intestinal CM production.
Proteomics‐based identification of two novel direct targets of hypoxia‐inducible factor‐1 and their potential roles in migration/invasion of cancer cells
Authors: Liao Shi‐Hua , Zhao Xu‐Yun , Han Yu‐Hui , Zhang Jing , Wang Li‐Shun , Xia Li , Zhao Ke‐Wen , Zheng Ying , Guo Meng , Chen Guo‐Qiang 
Published: July 2009

AbstractHypoxia‐inducible factor‐1 (HIF‐1), consisting of oxygen‐sensitive HIF‐1α and constitutively expressed HIF‐1β subunits, is a master transcriptional activator for cellular response to hypoxia. To explore direct HIF‐1 targets, here we used differential gel electrophoresis (DIGE) to compare the HIF‐1‐regulated proteins between leukemic U937T‐cell line with and without conditional induction of HIF‐1α protein by tetracycline‐off system. Among the upregulated proteins identified, mRNA levels of annexin A1, macrophage‐capping protein (CapG), S100 calcium‐binding protein A4 (S100A4), S100A11, acyl‐CoA‐binding protein and calcyclin‐binding protein also increased. The expressions of the annexin A1, CapG and S100A4 genes were significantly induced by hypoxia in five adherent cell lines tested besides U937 cells, while their expressions were blocked by the short hairpin RNA specifically against HIF‐1α. Further luciferase reporter assay and chromatin immunoprecipitation showed that HIF‐1α directly bound to three hypoxia‐responsive elements located at intron 1 of S100A4 gene and hypoxia‐responsive element at −350 to −346 of CapG gene, which are essential for HIF‐1‐induced expression. Additionally, the role of S100A4 expression in migration and invasion of cancer cells were also confirmed. These findings would provide new sights for understanding the molecular mechanisms underlying HIF‐1 action.
Photonic immobilization of high‐density protein arrays using Fourier optics
Authors: Skovsen Esben , Kold Ane B. , Neves‐Petersen Maria Teresa , Petersen Steffen B. 
Published: July 2009

AbstractThe technique of UV‐light‐assisted immobilization of disulfide containing proteins has been combined with the Fourier‐transforming properties of lenses as well as with a simple millimeter scale feature size spatial mask. The result is a new simple and inexpensive way of creating high‐density protein arrays with feature sizes down to a few hundred nanometers, which represents an improvement of tenfold over existing commercially available high‐density protein arraying methods.
Calpain‐truncated CRMP‐3 and ‐4 contribute to potassium deprivation‐induced apoptosis of cerebellar granule neurons
Authors: Liu Wei , Zhou Xing‐wang , Liu Shaojun , Hu Kunhua , Wang Chong , He Qingyu , Li Mingtao 
Published: July 2009

AbstractIncreasing evidence shows that calpain‐mediated proteolytic processing of a selective number of proteins plays an important role in neuronal apoptosis. Study of calpain‐mediated cleavage events and related functions may contribute to a better understanding of neuronal apoptosis and neurodegenerative diseases. We, therefore, investigated the role of calpain substrates in potassium deprivation‐induced apoptosis of cerebellar granule neurons (CGNs). Twelve previously known and seven novel candidates of calpain substrates were identified by 2‐D DIGE and MALDI‐TOF/TOF MS analysis. Further, the identified novel calpain substrates were validated by Western blot analysis. Moreover, we focused on the collapsin response mediator proteins (CRMP‐1, ‐2, ‐3 and ‐4 isoforms) and found that CRMPs were proteolytically processed by calpain but not by caspase, both in vivo and in vitro. To clarify the properties of the calpain‐mediated proteolysis of CRMPs, we constructed the deletion mutants of CRMPs for additional biochemical studies. In vitro cleavage assays revealed that CRMP‐1, ‐2 and ‐4 were truncated by calpain at the C‐terminus, whereas CRMP‐3 was cleaved at the N‐terminus. Finally, we assessed the role of CRMPs in the process of potassium deprivation‐triggered neuronal apoptosis by overexpressing the truncated CRMPs in CGNs. Our data clearly showed that the truncated CRMP‐3 and ‐4, but not CRMP‐1 and ‐2, significantly induced neuronal apoptosis. These findings demonstrated that calpain‐truncated CRMP‐3 and ‐4 act as pro‐apoptotic players when CGNs undergo apoptosis.
Accurate quantitation of standard peptides used for quantitative proteomics
Authors: Bordeerat Narisa K. , Georgieva Nadia I. , Klapper David G. , Collins Leonard B. , Cross Tyra J. , Borchers Christoph H. , Swenberg James A. , Boysen Gunnar 
Published: July 2009

AbstractMS‐based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC‐MS/MS (UPLC‐MS/MS) to accurately quantify peptide standards at concentrations of 0.1–10 μM. The ability for accurate quantitation of micro‐molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
Proteomic profiling of PrP27‐30‐enriched preparations extracted from the brain of hamsters with experimental scrapie
Authors: Giorgi Alessandra , Di Francesco Laura , Principe Serena , Mignogna Giuseppina , Sennels Lau , Mancone Carmine , Alonzi Tonino , Sbriccoli Marco , De Pascalis Angela , Rappsilber Juri , Cardone Franco , Pocchiari Maurizio , Maras Bruno , Schininà M. Eugenia 
Published: July 2009

AbstractTransmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrPTSE) of the host‐encoded cellular prion protein (PrPC). PrPTSE has a central role in the pathogenesis of the disease but other factors are likely involved in the pathological process. In this work we employed a multi‐step proteomic approach for the identification of proteins that co‐purify with the protease‐resistant core of PrPTSE (PrP27‐30) extracted from brains of hamsters with experimental scrapie. We identified ferritin, calcium/calmodulin‐dependent protein kinase α type II, apolipoprotein E, and tubulin as the major components associated with PrP27‐30 but also trace amounts of actin, cofilin, Hsp90α, the γ subunit of the T‐complex protein 1, glyceraldehyde 3‐phosphate dehydrogenase, histones, and keratins. Whereas some of these proteins (tubulin and ferritin) are known to bind PrP, other proteins (calcium/calmodulin‐dependent protein kinase α type II, Hsp90α) may associate with PrPTSE fibrils during disease. Apolipoprotein E and actin have been previously observed in association with PrPTSE, whereas cofilin and actin were shown to form abnormal rods in the brain of patients with Alzheimer disease. The roles of these proteins in the development of brain lesions are still unclear and further work is needed to explain their involvement in the pathogenesis of TSEs.
Getting a grip on proteomics data – Proteomics Data Collection (ProDaC)
Authors: Eisenacher Martin , Martens Lennart , Hardt Tanja , Kohl Michael , Barsnes Harald , Helsens Kenny , Häkkinen Jari , Levander Fredrik , Aebersold Ruedi , Vandekerckhove Joël , Dunn Michael J. , Lisacek Frederique , Siepen Jennifer A. , Hubbard Simon J. , Binz Pierre‐Alain , Blüggel Martin , Thiele Herbert , Cottrell John , Meyer Helmut E. , Apweiler Rolf , Stephan Christian 
Published: July 2009

AbstractIn proteomics, rapid developments in instrumentation led to the acquisition of increasingly large data sets. Correspondingly, ProDaC was founded in 2006 as a Coordination Action project within the 6th European Union Framework Programme to support data sharing and community‐wide data collection. The objectives of ProDaC were the development of documentation and storage standards, setup of a standardized data submission pipeline and collection of data. Ending in March 2009, ProDaC has delivered a comprehensive toolbox of standards and computer programs to achieve these goals.
Nature of the transient species formed in the pulse radiolysis of 4‐hydroxybenzyl alcohol in aqueous solutions: observation of equilibrium in the reaction of OH‐adducts with HPO ions
Authors: Dhiman Surajdevprakash B. , Naik Devidas B. 
Published: July 2009

AbstractReactions of .OH/O.− radicals, H‐atoms as well as specific oxidants such as N and Cl radicals with 4‐hydroxybenzyl alcohol (4‐HBA) in aqueous solutions have been investigated at various pH values using the pulse radiolysis technique. At pH 6.8, .OH radicals were found to react with 4‐HBA (k = 6 × 109 dm3 mol−1 s−1) mainly by contributing to the phenyl moiety and to a minor extent by H‐abstraction from the CH2OH group. .OH radical adduct species of 4‐HBA, i.e., .OH‐(4‐HBA) formed in the addition reaction were found to undergo dehydration to give phenoxyl radicals of 4‐HBA. Decay rate of the adduct species was found to vary with pH. At pH 6.8, decay was very much dependent on phosphate buffer ion concentrations. Formation rate of phenoxyl radicals was found to increase with phosphate buffer ion concentration and reached a plateau value of 1.6 × 105 s−1 at a concentration of 0.04 mol dm−3 of each buffering ion. It was also seen that .OH‐(4‐HBA) adduct species react with HPO ions with a rate constant of 3.7 × 107 dm3 mol−1 s−1 and there was no such reaction with H2PO ions. However, the rate of reaction of .OH‐(4‐HBA) adduct species with HPO ions decreased on adding KH2PO4 to the solution containing a fixed concentration of Na2HPO4 which indicated an equilibrium in the H+ removal from .OH‐(4‐HBA) adduct species in the presence of phosphate ions. In the acidic region, the .OH‐(4‐HBA) adduct species were found to react with H+ ions with a rate constant of 2.5 × 107 dm3 mol−1 s−1. At pH 1, in the reaction of .OH radicals with 4‐HBA (k = 8.8 × 109 dm3 mol−1 s−1), the spectrum of the transient species formed was similar to that of phenoxyl radicals formed in the reaction of Cl radicals with 4‐HBA at pH 1 (k = 2.3 × 108 dm3 mol−1 s−1) showing that .OH radicals quantitatively bring about one electron oxidation of 4‐HBA. Reaction of .OH/O.− radicals with 4‐HBA by H‐abstraction mechanism at neutral and alkaline pH values gave reducing radicals and the proportion of the same was determined by following the extent of electron transfer to methyl viologen. H‐atom abstraction is the major pathway in the reaction of O.− radicals with 4‐HBA compared to the reaction of .OH radicals with 4‐HBA. At pH 1, transient species formed in the reactions of H‐atoms with 4‐HBA (k = 2.1 × 109 dm3 mol−1 s−1) were found to transfer electrons to methyl viologen quantitatively. Copyright © 2009 John Wiley & Sons, Ltd.
Intramolecular Diels–Alder reaction in enyne–allenes: a computational investigation and comparison with the Myers–Saito and Schmittel reactions
Authors: Chen Hsin‐Tsung , Chen Hui‐Lung , Ho Jia‐Jen 
Published: July 2009

AbstractThe reaction mechanisms as well as substituted effect and solvent effect of the enyne–allenes are investigated by Density Functional Theory (DFT) method and compared with the Myers–Saito and Schmittel reactions. The Myers–Saito reaction of non‐substituted enyne–allenes is kinetically and thermodynamically favored as compared to the Schmittel reaction; while the concerted [4 + 2] cycloaddition is only 1.32 kcal/mol higher than the C2C7 cyclization and more exothermic (ΔRE = −69.38 kcal/mol). For R1 = CH3 and t‐Bu, the increasing barrier of the C2C7 cyclization is higher than that for the C2C6 cyclization because of the steric effect, so the increased barrier of the [4 + 2] cycloaddition is affected by such substituted electron‐releasing group. Moreover, the strong steric effect of R1 = t‐Bu would shift the C2C7 cyclization to the [4 + 2] cycloaddition. On the other hand, for R1 = Ph, NH2, O−, NO2, and CN substituents, the barrier of the C2C6 cyclization would be more diminished than the C2C7 cyclization due to strong mesomeric effect; the reaction path of C2C7 cyclization would also shift to the [4 + 2] cycloaddition. The solvation does not lead to significant changes in the potential‐energy surface of the reaction except for the more polar surrounding solvent such as dimethyl sulfoxide (DMSO), or water. Copyright © 2009 John Wiley & Sons, Ltd.
Impact of alopecia and scalp cooling on the well‐being of breast cancer patients
Authors: van den Hurk Corina J. G. , Mols Floortje , Vingerhoets Ad J. J. M. , Breed Wim P. M. 
Published: July 2009

AbstractObjective: Chemotherapy‐induced alopecia, a common and distressing side effect of chemotherapy, may be prevented by scalp cooling, which reduces toxicity of cytostatics in hair root cells. This is the first study designed to assess the effect of scalp cooling on well‐being.Methods: A prospective multi‐centre study was performed in 13 hospitals. Breast cancer patients treated with (N=98) and without (N=168) scalp cooling completed questionnaires (EORTC QLQ‐C30 and EORTC‐QLQ‐BR23, BIS, MBA, HADS) before chemotherapy, and three weeks and six months after the last chemotherapy cycle.Results: Scalp cooling was effective in 52% of the cases. Alopecia was considered among the most distressing problems at all three moments of measurement. A trend towards higher well‐being was found in successfully scalp‐cooled patients, as indicated by a general better health‐related quality of life and better body image, whereas unsuccessfully scalp‐cooled patients reported lowest well‐being.Conclusions: Scalp cooling contributes not only to the well‐being of successfully scalp‐cooled patients but also seems to cause additional distress when patients lose their hair despite scalp cooling. This might be related to disappointment due to alopecia despite scalp cooling or possibly to a general higher biological availability of cytostatics. We recommend additional support for patients when scalp cooling is not successful and to spend more effort to maximise the effectiveness of scalp cooling. Copyright © 2009 John Wiley & Sons, Ltd.
Ice‐wedge pseudomorphs and frost‐cracking structures in Weichselian sediments, central‐west Poland
Authors: Ewertowski Marek 
Published: July 2009

AbstractWedge‐shaped structures are described from three sites in the central Wielkopolska region (central‐west Poland) in two main positions: (1) within fluvioglacial deposits below the Weichselian subglacial till and (2) within recessional deposits above this subglacial till. The wedges are interpreted as ice‐wedge pseudomorphs (Rensko site), relict frost cracks (Tomice site) and thermokarst‐affected ice‐wedge pseudomorphs (Annowo‐Kiszkowo area). The pseudomorphs suggest that thermal‐contraction cracking occurred in the region in front of the advancing Weichselian ice sheet, as well as following its recession. Frost structures, which are interpreted as having degraded slowly, were much more affected by thermokarst processes (Annowo‐Kiszkowo area) than those in the Rensko and Tomice sites which probably thawed rapidly, due to an insulating and warming effect of the overlying ice masses. The development of ice wedges was influenced by wet conditions in former depressions between ice‐cored ridges or by large amounts of meltwater in the proglacial environment. These periglacial features support previous hypotheses that climatic conditions in the Wielkopolska region after the last ice‐sheet recession changed gradually from glacial to periglacial. Copyright © 2009 John Wiley & Sons, Ltd.
Combined structural and optical studies of stacking faults in 4H‐SiC layers grown by chemical vapour deposition
Authors: Marinova Maya , Robert Teddy , Juillaguet Sandrine , Tsiaoussis Ioannis , Frangis Nikolaos , Polychroniadis Efstathios , Camassel Jean , Chassagne Thierry 
Published: July 2009

AbstractThe formation of in‐grown stacking faults (SFs) in chemical vapour deposition (CVD) grown 4H‐SiC epilayer has been studied by high‐resolution transmission electron microscopy (HRTEM) and low‐temperature photoluminescence (LTPL). Local inhomogeneities in the SF density have been found, where different SF arrangements appear. They range from pure 8H‐SiC unit cells to a few distinguished sequences, forming in some cases long‐range semi‐periodic incommensurate structures. Despite such large dispersion, the same optical (LTPL) signature is always found. This is discussed in the light of coupled quantum well models.magnified imageHRTEM image showing the formation of an extended defect (16 bilayers thick) in a CVD grown 4H‐SiC epitaxial layer.
Structural changes and peculiarities of NiPdSi formation investigated by Raman and Auger spectroscopy
Authors: Karabko Aliaksandra , Dostanko Anatoly , Kong JinFang , Shen Wenzhong 
Published: July 2009

AbstractIn this contribution we report on peculiarities of formation, structural and vibrational properties of compositional (alloying) nickel silicides such as NiPdSi. MicroRaman analysis has shown that NiPdSi films are thermally stable up to 900 °C, which is by 100 °C higher than that for pure NiSi films. Using Miedema's model we have calculated the heat of formation for NiPdSi to be by 4 kJ mol−1 smaller than that for pure NiSi. This demonstrates an essential role of Pd in the enhancement of thermal stability of NiSi and in retardation of NiSi2 phase formation. Raman spectra registered for NiPdSi show the peaks at wavenumbers typical for NiSi, although red shifted, as well as new Raman peaks at 322 and 434 cm−1 reported recently in [Karabko et al. J. Appl. Phys. 105, 033518 (2009)]. We have assigned these two peaks to the compositional disorder, introduced by the presence of Pd alloy. By means of Auger electron spectroscopy (AES) we have determined the peculiarities of the element distribution and Pd diffusion in NiPdSi and NiSi films, which results in a slight lack of silicon in the stoichiometry of phases and exponential decay law of Pd distribution with temperature.
Multi‐layer thickness determination using differential‐based enhanced Fourier transforms of X‐ray reflectivity data
Authors: Poust Benjamin , Sandhu Rajinder , Goorsky Mark 
Published: July 2009

AbstractLayer thickness determination of single and multi‐layer structures is achieved using a new method for generating Fourier transforms (FTs) of X‐ray reflectivity data. This enhanced Fourier analysis is compared to other techniques in the determination of AlN layer thickness deposited on sapphire. In addition to demonstrably improved results, the results also agree with thicknesses determined using simulations and TEM measurements. The effectiveness of the technique is further demonstrated using the more complicated metamorphic epitaxial multi‐layer AlSb/InAs structures deposited on GaAs. The approach reported here is based upon differentiating the specular intensity with respect to the vertical reciprocal space coordinate QZ. In general, differentiation is far more effective at removing the sloping background present in reflectivity scans than logarithmic compression alone, average subtraction alone, or other methods. When combined with any of the other enhancement techniques, however, differentiation yields distinguishable discrete Fourier transform (DFT) power spectrum peaks for even the weakest and most truncated of sloping oscillations that are present in many reflectivity scans from multi‐layer structures.