bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monoamine oxidase inhibitory activity in tobacco smoke varies with tobacco type
Authors: Lewis Amy J , Truman Penelope , Hosking Matthew R , Miller John H 
Published: June 2011

BackgroundIt has been suggested that inhibition of monoamine oxidase (MAO) activity by components of cigarette smoke may impact on smoking addiction, but it is unclear to what extent the known MAO inhibitors in tobacco smoke cause this inhibition.MethodsMAO inhibitory activity was measured in a series of tobacco particulate matter preparations from different brands of cigarette and loose-leaf tobacco commonly available in New Zealand.ResultsWhen tobacco extracts were diluted to contain a physiologically relevant nicotine concentration of 0.2 μM, all samples tested inhibited MAO-A and MAO-B by between 4% and 12% in a standard assay. Per mg of nicotine, samples from factory-made cigarettes contained significantly less MAO inhibitory activity than did samples from loose-leaf tobacco. When inhibitory activity was calculated on a per mg of tar basis, there was no significant difference between loose-leaf tobaccos and factory-made cigarettes.ConclusionsThe present study shows that the ratio of nicotine to MAO inhibitory activity varies depending on the type of tobacco product used. The roll-your-own tobaccos tested delivered more tar and more MAO inhibitory activity per mg of nicotine than the factory-made cigarettes. These findings suggest that smokers of roll-your-own tobacco may experience greater difficulty in stopping smoking.
Through tobacco industry eyes: civil society and the FCTC process from Philip Morris and British American Tobacco's perspectives
Authors: Gonzalez Mariaelena , Green Lawrence W , Glantz Stanton A 
Published: June 2011

ObjectiveTo analyse the models Philip Morris (PM) and British American Tobacco (BAT) used internally to understand tobacco control non-governmental organizations (NGOs) and their relationship to the global tobacco control policy-making process that resulted in the Framework Convention for Tobacco Control (FCTC).MethodsAnalysis of internal tobacco industry documents in the Legacy Tobacco Document Library.ResultsPM contracted with Mongoven, Biscoe, and Duchin, Inc. (MBD, a consulting firm specialising in NGO surveillance) as advisors. MBD argued that because NGOs are increasingly linked to epistemic communities, NGOs could insert themselves into the global policy-making process and influence the discourse surrounding the treaty-making process. MBD advised PM to insert itself into the policy-making process, mimicking NGO behaviour. BAT's Consumer and Regulatory Affairs (CORA) department argued that global regulation emerged from the perception (by NGOs and governments) that the industry could not regulate itself, leading to BAT advocating social alignment and self-regulation to minimise the impact of the FCTC. Most efforts to block or redirect the FCTC failed.ConclusionsPM and BAT articulated a global policy-making environment in which NGOs are key, non-state stakeholders, and as a result, internationalised some of their previous national-level strategies. After both companies failed to prevent the FCTC, their strategies began to align. Multinational corporations have continued to successfully employ some of the strategies outlined in this paper at the local and national level while being formally excluded from ongoing FCTC negotiations at the global level.
Epidemiological and molecular characterization ofStaphylococcus haemolyticusstrains, from a hematology ward, with decreased susceptibility to glycopeptides
Authors: Ma Xiao Xue , Sun Dan Dan , Hu Jian , Wang En Hua , Luo En Jie 
Published: June 2011

In the present study, we report on the reduced susceptibility to teicoplanin among clinical isolates of Staphylococcus haemolyticus in a hematology ward of a teaching hospital. The molecular characterization of 17 S. haemolyticus strains was performed using mec gene complex classification, pulsed-field gel electrophoresis analysis, and minimum inhibitory concentration examination. Pulsotype A strains carrying a class C2 mec gene complex were the most prevalent strains, at 64.7%. In vivo selection of stepwise increase in resistance to vancomycin and teicoplanin was observed in three S. haemolyticus strains serially isolated from a case patient. The results of the present study suggest the regional spread of certain S. haemolyticus clones with diminished susceptibility to glycopeptides, emphasizing the need for continuous monitoring of minimum inhibitory concentration levels of vancomycin and teicoplanin in S. haemolyticus strains, and the importance of infection control practices to prevent its transmission.
Echo response to chirping in the weakly electric brown ghost knifefish (Apteronotus leptorhynchus): role of frequency and amplitude modulations
Authors: Gama Salgado José Antonio , Zupanc Günther K.H. 
Published: June 2011

Teleost fish of the order Gymnotiformes are distinguished by their ability to produce electric discharges by means of specialized organs. These electric organ discharges serve various behavioral functions, including communication. During such electric interactions, male brown ghost knifefish ( Apteronotus leptorhynchus (Ellis in Eigenmann, 1912)) generate several types of transient frequency and amplitude modulations (“chirps”) of the otherwise nearly constant discharges. Previous studies have shown that the chirps generated by one individual follow those of the other with a preferred latency of approximately 500–1000 ms. As demonstrated in the present study, signals consisting of either frequency modulations or amplitude modulations are able to trigger this echo response. Signals composed of just amplitude modulations are effective in triggering an echo response only if the reduction in amplitude is large (approximately 40%, relative to baseline of the electric organ discharge of the emitting fish). By contrast, in frequency-modulated signals, a maximum frequency increase as small as 1.2% relative to baseline frequency is sufficient to trigger an echo response. This remarkable sensitivity might be an adaptation for the detection of so-called type-2 chirps, as chirps of this type are composed of rather small frequency increases and negligible amplitude modulations. In line with this hypothesis is the observation that during electric interactions of two fish, the generation of these chirps dominates the production of any of the other five chirp types known.
Mapping potential core areas for lynx (Lynx canadensis) using pellet counts from snowshoe hares (Lepus americanus) and satellite imagery
Authors: McCann N.P. , Moen R.A. 
Published: June 2011

We used location data from radio-collared Canada lynx ( Lynx canadensis Kerr, 1792), pellet-count data from snowshoe hares ( Lepus americanus Erxleben, 1777), and cover-type data from satellite imagery to evaluate the relationship between the scale of habitat measurement and the potential for persistence of lynx in northeastern Minnesota, USA, at the southern extent of their range. We counted hare pellets at transects throughout northeastern Minnesota to index hare abundance in cover types. Pellet counts were highest in coniferous forest, regenerating–young forest, and shrubby grassland, and these cover types were greater inside lynx use areas than outside of them. Proportions of regenerating–young forest were greater at scales ≥5 km2. We used these results and satellite imagery to map potential lynx core areas. We predicted that 7%–20% of the study area was suitable for lynx. Areas that we predicted to be suitable for lynx corresponded with known core areas, including those withheld from analyses. To maintain habitat for lynx persistence, forest management should retain current levels of 10- to 30-year-old coniferous forest and include ≥5 km2 areas containing 40% of 10- to 30-year-old coniferous forest. Mapping of potential core areas would be improved if cover-type data from satellite imagery identified conifer regeneration.
Avian nest success, mammalian nest predator abundance, and invertebrate prey availability in a fragmented landscape
Authors: Richmond S. , Nol E. , Burke D. 
Published: June 2011

Avian nest success is largely determined by predation, but factors affecting the abundance of potential nest predators are rarely studied. We used an information–theoretic approach to assess relative support for models including invertebrate biomass, mammalian nest predator abundance, and percent cover at nests as explanatory variables for nest success of Ovenbirds ( Seiurus aurocapilla (L., 1766)) and Wood Thrush ( Hylocichla mustelina (J.F. Gmelin, 1789)). We ranked models including local vegetation characteristics and landscape composition at two spatial scales (100 and 2000 m) as explanatory variables for the abundance of mammalian nest predator groups and for prey biomass. The nest success of Ovenbirds was best explained by a positive association with percent cover by forbs and seedlings, whereas a positive relationship with prey biomass best explained the nest success of Wood Thrush. Most mammal genera were associated with landscape composition within 100 m of the study sites, and most were positively associated with housing density. Prey biomass was best explained by a positive association with less intensive agriculture within 2000 m. Implementing silvicultural techniques that preserve important habitat features within fragmented forests, limiting housing density within 100 m, and increasing the amount of less intensive agriculture within 2000 m of forest fragments may improve nest success for forest songbirds.
Implications of a high-energy and low-protein diet on the body composition, fitness, and competitive abilities of black (Ursus americanus) and grizzly (Ursus arctos) bears
Authors: McLellan B.N. 
Published: June 2011

Plants are not ideal foods for bears yet many populations are largely vegetarian. Implications of this diet on the body composition, fitness, and competiveness of black ( Ursus americanus Pallas, 1780) and grizzly ( Ursus arctos L., 1758) bears have had limited field investigation. The analysis of scats of grizzly and black bears from the Flathead valley, British Columbia, suggest seasonal dietary differences between species, but >85% of the summer diet of both species were fruits that are low in protein. Body composition measurements showed bears loose fat during spring, gained fat during summer, and grizzly bears were leaner than black bears. Individual black bears gained mass up to 2.7 times faster than theory predicted. Bears rapidly gained fat but lost lean tissues while feeding on fruit, suggesting that lean tissues were used to buffer seasonal protein shortages. Comparisons among populations of grizzly bears without access to salmon revealed the amount of meat in the diet was positively related with adult female mass but negatively related with bear density. Bears have the behavioural and phenotypic plasticity which enables populations that focus their foraging on plants to have small but fat females and live at higher densities than populations that focus more on obtaining terrestrial meat.
Habitat-specific distribution and abundance of arctic ground squirrels (Urocitellus parryii plesius) in southwest Yukon
Authors: Donker S.A. , Krebs C.J. 
Published: June 2011

Arctic ground squirrels ( Urocitellus parryii plesius (Osgood, 1900); formerly Spermophilus parryii plesius Osgood, 1900) were studied in three distinct habitat types (boreal forest, low-elevation meadows, and alpine meadows) in the Kluane region of the southwest Yukon Territory, Canada, from 2008 to 2010 to determine if populations in these different habitats provide evidence for habitat-specific distribution and abundance. Abundance in the boreal forest has been shown to be synchronous with the cycle of snowshoe hares ( Lepus americanus Erxleben, 1777) in the region owing to shared predators. We predicted that populations in the boreal forest would be low because of the current low phase in the cycle of snowshoe hares, and that in low-altitude meadows and alpine meadows, ground squirrels would be relatively abundant. Late-summer densities differed significantly between habitat types with 0.38 ± 0.13 squirrel/ha (mean ± 1 SE) in boreal-forest habitat, 1.25 ± 0.22 squirrel/ha in low-altitude-meadow habitat, and 5.7 ± 0.22 squirrels/ha in alpine-meadow habitat. In 2009, populations were extirpated from boreal-forest habitat, while densities in low-elevation meadows and alpine meadows were 1.6 ± 0.34 squirrel/ha and 6.1 ± 0.7 squirrels/ha, respectively. The current absence of squirrels from the boreal forest and the persistence of populations in low-elevation-meadow and alpine-meadow habitat suggest that source–sink dynamics may exist between boreal-forest and meadow habitat types.
Positive Feedback Loop Between PI3K-Akt-mTORC1 Signaling and the Lipogenic Pathway Boosts Akt Signaling: Induction of the Lipogenic Pathway by a Melanoma Antigen
Authors: Yamauchi Yoshio , Furukawa Keiko , Hamamura Kazunori , Furukawa Koichi 
Published: June 2011

Abstract
The lipogenic phenotype is a metabolic hallmark of cancer cells. Sterol regulatory element–binding proteins (SREBP) are key transcriptional factors to regulate biosynthesis of cholesterol and fatty acids. It has been poorly understood how the lipogenic phenotype in cancer cells is regulated and how it augments their malignant properties. Here we describe roles of the melanoma antigen ganglioside GD3 and phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR complex 1 (mTORC1) signaling in the regulation of SREBP activity, cholesterol biosynthesis, and the integrity of lipid rafts in human melanoma cells. GD3 expression induced the activation of both SREBP-1 and SREBP-2. Consequently, HMG-CoA reductase expression and cholesterol biosynthesis increased. The activation of the SREBP pathway was independent of the oncogenic BRAF mutation. On the other hand, it was regulated by PI3K-Akt-mTORC1 signaling in human melanoma cells. Disruption of the signaling pathway resulted in the reduction of cholesterol in lipid rafts. Inhibition of the SREBP pathway attenuated Akt activation in lipid rafts and suppressed the growth of human melanoma cells in vitro and in vivo. These results suggest that PI3K-Akt-mTORC1 signaling is important for the integrity of lipid rafts by regulating SREBP activation and subsequent cholesterogenesis. We thus propose a positive feedback circuit in which PI3K-Akt-mTORC1-SREBP signaling boosts Akt signaling in human melanoma cells expressing GD3. Cancer Res; 71(14); 4989–97. ©2011 AACR.
Notch Signaling Activated by Replication Stress–Induced Expression of Midkine Drives Epithelial–Mesenchymal Transition and Chemoresistance in Pancreatic Cancer
Authors: Güngör Cenap , Zander Hilke , Effenberger Katharina E. , Vashist Yogesh K. , Kalinina Tatyana , Izbicki Jakob R. , Yekebas Emre , Bockhorn Maximilian 
Published: June 2011

Abstract
The incidence of pancreatic ductal adenocarcinoma (PDAC) nearly equals its mortality rate, partly because most PDACs are intrinsically chemoresistant and thus largely untreatable. It was found recently that chemoresistant PDAC cells overexpress the Notch-2 receptor and have undergone epithelial–mesenchymal transition (EMT). In this study, we show that these two phenotypes are interrelated by expression of Midkine (MK), a heparin-binding growth factor that is widely overexpressed in chemoresistant PDAC. Gemcitabine, the front-line chemotherapy used in PDAC treatment, induced MK expression in a dose-dependent manner, and its RNAi-mediated depletion was associated with sensitization to gemcitabine treatment. We identified an interaction between the Notch-2 receptor and MK in PDAC cells. MK–Notch-2 interaction activated Notch signaling, induced EMT, upregulated NF-κB, and increased chemoresistance. Taken together, our findings define an important pathway of chemoresistance in PDAC and suggest novel strategies for its clinical attack. Cancer Res; 71(14); 5009–19. ©2011 AACR.
Prediagnostic Serum Levels of Cytokines and Other Immune Markers and Risk of Non-Hodgkin Lymphoma
Authors: Purdue Mark P. , Lan Qing , Bagni Rachel , Hocking William G. , Baris Dalsu , Reding Douglas J. , Rothman Nathaniel 
Published: June 2011

AbstractAlthough severe immune dysregulation is an established risk factor for non-Hodgkin lymphoma (NHL), it is unclear whether subclinical immune system function influences lymphomagenesis. To address this question, we conducted a nested case–control study within the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial to investigate whether circulating levels of cytokines and other immune markers are associated with future risk of NHL. Selected cytokines [interleukin (IL)-4, IL-6, IL-10, and TNF-α] and other immune markers [soluble TNF receptor 1 (sTNF-R1), sTNF-R2, C-reactive protein, and sCD27] were measured in prediagnostic serum specimens from 297 incident NHL cases and 297 individually matched controls. ORs and 95% confidence intervals (CI) relating quartiles of analyte concentration to NHL risk were calculated by using conditional logistic regression. Statistically significant associations with increased NHL risk were observed for elevated serum levels of sTNF-R1 (quartile 4 vs. quartile 1: OR = 1.7, 95% CI: 1.1–2.8; Ptrend = 0.02) and sCD27 (OR = 5.3, 95% CI: 2.9–9.4; Ptrend &lt; 0.0001). These associations remained in analyses of cases diagnosed longer than 6 years following blood collection (sTNF-R1: OR = 2.1, 95% CI: 1.0–4.0, Ptrend = 0.01; sCD27: OR = 4.1, 95% CI: 1.9–8.5, Ptrend = 0.0001). Elevated levels of IL-10, TNF-α and sTNF-R2 were also significantly associated with increased risk of NHL overall; however, these associations weakened with increasing time from blood collection to case diagnosis and were null for cases diagnosed longer than 6 years postcollection. Our findings for sTNF-R1 and sCD27, possible markers for inflammatory and B-cell stimulatory states, respectively, support a role for subclinical inflammation and chronic B-cell stimulation in lymphomagenesis. Cancer Res; 71(14); 4898–907. ©2011 AACR.
Genetic Variations in Multiple Drug Action Pathways and Survival in Advanced Stage Non–Small Cell Lung Cancer Treated with Chemotherapy
Authors: Li Yafei , Sun Zhifu , Cunningham Julie M. , Aubry Marie C. , Wampfler Jason A. , Croghan Gary A. , Johnson Cassandra , Wu Danli , Aakre Jeremiah A. , Molina Julian , Wang Liewei , Pankratz V. Shane , Yang Ping 
Published: June 2011

Abstract
Purpose: Variations in genes related to biological activity of anticancer drugs could influence treatment responses and lung cancer prognosis. Genetic variants in four biological pathways, that is, glutathione metabolism, DNA repair, cell cycle, and epidermal growth factor receptor (EGFR), were systematically investigated to examine their association with survival in advanced stage non–small cell lung cancer (NSCLC) treated with chemotherapy.
Experimental Design: A total of 894 tagging single-nucleotide polymorphisms (SNP) in 70 genes from the four pathways were genotyped and analyzed in a 1,076-patient cohort. Association with overall survival was analyzed at SNP and whole-gene levels within all patients and major chemotherapy agent combination groups.
Results: A poorer overall survival was observed in patients with genetic variations in GSS (glutathione pathway) and MAP3K1 (EGFR pathway; HR = 1.45; 95% CI = 1.20–1.77 and HR = 1.25; 95% CI = 1.05–1.50, respectively). In the stratified analysis on patients receiving platinum plus taxane treatment, we observed a hazardous effect on overall survival by the MAP3K1 variant (HR = 1.38; 95% CI = 1.11–1.72) and a protective effect by RAF1 (HR = 0.64; 95% CI = 0.50–0.82) in the EGFR pathway. In patients receiving platinum plus gemcitabine treatment, RAF1 and GPX5 (glutathione pathway) genetic variations showed protective effects on survival (HR = 0.54; 95% CI = 0.38–0.77; HR = 0.67; 95% CI = 0.52–0.85, respectively); in contrast, NRAS (EGFR pathway) and GPX7 (glutathione pathway) variations showed hazardous effects on overall survival (HR = 1.91; 95% CI = 1.30–2.80; HR = 1.83; 95% CI = 1.27–2.63, respectively). All genes that harbored these significant SNPs remained significant by whole-gene analysis.
Conclusion: Common genetic variations in genes of EGFR and glutathione pathways may be associated with overall survival among patients with advanced stage NSCLC treated with platinum, taxane, and/or gemicitabine combinations. Clin Cancer Res; 17(11); 3830–40. ©2011 AACR.
Prognostic and Predictive Role of Lactate Dehydrogenase 5 Expression in Colorectal Cancer Patients Treated with PTK787/ZK 222584 (Vatalanib) Antiangiogenic Therapy
Authors: Koukourakis Michael I. , Giatromanolaki Alexandra , Sivridis Efthimios , Gatter Kevin C. , Trarbach Tanja , Folprecht Gunnar , Shi Michael M. , Lebwohl David , Jalava Tarja , Laurent Dirk , Meinhardt Gerold , Harris Adrian L. 
Published: June 2011

Abstract
Purpose: The Colorectal Oral Novel therapy For the Inhibition of angiogenesis and Retarding of Metastases (CONFIRM)-randomized trials, investigating the role of the VEGF-receptor inhibitor PTK787/ZK 222584 (vatalanib) in colorectal cancer (FOLFOX 4 ± vatalanib), showed some benefit in patients with high serum lactate dehydrogenase (LDH) levels. Here, we investigated the expression of LDH5 (encoded entirely by the LDHA gene, regulated by the hypoxia inducible factors) in cancer tissues from patients recruited in the CONFIRM trials and relationship to response.
Experimental Design: Paraffin-embedded materials from 179 patients recruited in the CONFIRM trials were analyzed by immunohistochemistry for the expression of the LDH5 protein. Correlations with serum LDH, response, and survival were assessed.
Results: A significant association of tumor burden and of poor performance status (PS) with serum LDH was noted. Poor PS and high tumor LDH5 expression predicted for poor response rates. High tissue LDH5 was related to poor progression-free survival (PFS) only in the placebo group of patients, whereas the addition of vatalanib seemed to improved response and PFS in this subgroup. High serum LDH levels were linked with significantly poorer overall survival, which however was not sustained in multivariate analysis.
Conclusions: Serum LDH and tissue LDH5 levels are complementary features that help to characterize the activity of LDH in colorectal cancer and have a potent value in predicting response to chemotherapy. The addition of vatalanib diminished the impact of LDH expression on the prognosis of patients. Clin Cancer Res; 17(14); 4892–900. ©2011 AACR.