bluuebunny/crossref_metadata_2025_split huggingface dataset:
The factor structure of a Chinese Geriatric Depression Scale‐SF: use with alone elderly Chinese in Shanghai, China
Authors: Lai Daniel , Tong Hongmei , Zeng Qun , Xu Wenyan 
Published: August 2009

AbstractObjectiveThis study aimed to examine the factor structure of a Chinese version of the 15‐item Geriatric Depression Scale (GDS) with a sample of community dwelling elderly Chinese living alone in Shanghai, China.MethodData were obtained between August and October 2008 through face‐to‐face interviews, using a structured survey questionnaire, from a random sample of 228 Chinese who were 60 years and older and living alone in one of the aging communities in Shanghai, China. Depressive symptoms were measured by a 15‐item Chinese version Geriatric Depression Scale. Both exploratory factor analysis and confirmatory factor analysis were conducted to examine the factor structure of the GDS.ResultsOver 30% of the elderly Chinese living alone reported having symptoms that indicated that they had mild or an above mild level of depression. Furthermore, the findings also indicated that the depression symptoms were loaded into a four‐factor model: 1) positive and negative mood; 2) energy level; 3) inferiority; and 4) disinterested, explaining over 58% of the total variance of depressive symptoms.ConclusionsThe findings presented evidence of the applicability of the GDS to elderly Chinese living alone in China. This instrument would be useful for identifying potential depression concerns among elderly Chinese living alone. Copyright © 2009 John Wiley & Sons, Ltd.
Transgenic expression of human wild‐type amyloid precursor protein decreases neurogenesis in the adult hippocampus
Authors: Naumann Nicole , Alpár Alán , Ueberham Uwe , Arendt Thomas , Gärtner Ulrich 
Published: August 2009

AbstractBesides its role in Alzheimer's disease, the amyloid precursor protein (APP) is implicated in several physiological functions in neuronal tissue such as cell survival, neurite outgrowth, synaptic formation, and neuronal plasticity. The present study analyzed effects of human wild‐type APP (hAPP) overexpression on adult hippocampal neurogenesis in transgenic mice. Mice were housed under either standard or enriched conditions, the latter to boost neurogenetic activity. Different aspects of neurogenesis including proliferation, survival, and differentiation were assessed by employing the BrdU‐incorporation method and, in parallel, immunohistochemistry for the neuronal and glial markers NeuN and S100b, respectively. Overexpression of hAPP caused a significant decrease in cell proliferation under standard housing conditions. The relative increase in the proliferation rate following housing in enriched environment was not different to that observed in wild‐type mice. Overexpression of hAPP, on the other hand, promoted the survival of newly generated cells, but just under conditions of standard housing. Findings further suggest that overexpression of hAPP suppresses the phenotypic shift toward neuronal differentiation under conditions of enriched environment. In summary, the results reveal a dual effect of APP on adult hippocampal neurogenesis, comprising antiproliferative and prosurvival activities. © 2009 Wiley‐Liss, Inc.
Spectral analysis of electrocorticographic activity during pharmacological preconditioning and seizure induction by intrahippocampal domoic acid
Authors: Sawant P.M. , Mountfort D.O. , Kerr D.S. 
Published: August 2009

AbstractPreviously we have shown that low‐dose domoic acid (DA) preconditioning produces tolerance to the behavioral effects of high‐dose DA. In this study, we used electrocorticography (ECoG) to monitor subtle CNS changes during and after preconditioning. Young adult male Sprague‐Dawley rats were implanted with a left cortical electrode, and acute recordings were obtained during preconditioning by contralateral intrahippocampal administration of either low‐dose DA (15 pmoles) or saline, followed by a high‐dose DA (100 pmoles) challenge. ECoG data were analyzed by fast Fourier transformation to obtain the percentage of baseline power spectral density (PSD) for delta to gamma frequencies (range: 1.25–100 Hz). Consistent with previous results, behavioral analysis confirmed that low‐dose DA preconditioning 60 min before a high‐dose DA challenge produced significant reductions in cumulative seizure scores and high level seizure behaviors. ECoG analysis revealed significant reductions in power spectral density across all frequency bands, and high‐frequency/high‐amplitude spiking in DA preconditioned animals, relative to saline controls. Significant correlations between seizure scores and ECoG power confirmed that behavioral analysis is a reliable marker for seizure analysis. The reduction ofpower in delta to gamma frequency bands in contralateral cortex does not allow a clear distinction between seizure initiation and seizure propagation, but does provide objective confirmation that pharmacological preconditioning by DA reduces network seizure activity. © 2009 Wiley‐Liss, Inc.
Preventive effects of etodolac, a selective cyclooxygenase‐2 inhibitor, on cancer development in extensive metaplastic gastritis, a Helicobacter pylori‐negative precancerous lesion
Authors: Yanaoka Kimihiko , Oka Masashi , Yoshimura Noriko , Deguchi Hisanobu , Mukoubayashi Chizu , Enomoto Shotaro , Maekita Takao , Inoue Izumi , Ueda Kazuki , Utsunomiya Hirotoshi , Iguchi Mikitaka , Tamai Hideyuki , Fujishiro Mitsuhiro , Nakamura Yasushi , Tsukamoto Tetsuya , Inada Kenichi , Takeshita Tatsuya , Ichinose Masao 
Published: August 2009

AbstractThe present study investigated the preventive effects of etodolac, a selective cyclo‐oxygenase (COX)‐2 inhibitor, on metachronous cancer development after endoscopic resection of early gastric cancer. Among 267 early gastric cancer patients who underwent endoscopic resection, 47 patients with extensive metaplastic gastritis were selected based on endoscopic findings and our previously described criteria of serum pepsinogen (PG) test‐positive and Helicobacter pylori antibody‐negative conditions. Nonrandomized etodolac treatment (300 mg/day) was administered to 26 patients (Group A), while the remaining 21 patients were untreated (Group B). No significant differences in age, sex distribution, lifestyle factors or extent of metaplastic gastritis at baseline were identified between groups. Patients were followed for metachronous cancer development with endoscopy every 6–12 months for up to 5 years. Mean (standard deviation) follow‐up period was 4.2 (0.9) years. In Group B, 5 cancers developed (incidence rate = 6,266/100,000 person‐years), significantly more than the 1 cancer in Group A (incidence rate = 898/100,000 person‐years; p < 0.05). Long‐term etodolac treatment did not influence the extent of metaplastic gastritis as revealed by endoscopic findings or by serum PG levels, but effectively reduced metachronous cancer development in patients with extensive metaplastic gastritis. These results strongly suggest that chemoprevention of cancer in the metaplastic stomach is possible by controlling COX‐2 expression.
Dickkopf‐1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth
Authors: Takahashi Nobuyasu , Fukushima Tsuyoshi , Yorita Kenji , Tanaka Hiroyuki , Chijiiwa Kazuo , Kataoka Hiroaki 
Published: August 2009

AbstractThe protein products of the Dickkopf (DKK) genes are antagonists of Wnt glycoproteins, which participate in tumor development and progression by binding to frizzled receptors. In this study, the expression of DKK‐1 was analyzed in a panel of 43 human cultured carcinoma cell lines. DKK‐1 expression was consistently and significantly upregulated in pancreatic carcinoma cell lines. Low level of DKK‐3 expression was also seen. In contrast, the expression of DKK‐2 and ‐4 was not detectable in most pancreatic carcinoma cell lines. The overexpression of DKK‐1 was confirmed in surgically resected human pancreatic cancer tissues, in which the mRNA level was evaluated in paired samples from cancerous and noncancerous pancreatic tissues. In ductal adenocarcinomas (23 cases), DKK‐1 mRNA levels were significantly upregulated compared to corresponding noncancerous tissues in a statistically significant level. To test the biological role of DKK‐1 in pancreatic carcinoma cells, we performed a knockdown of DKK‐1 in SUIT‐2 human pancreatic adenocarcinoma cell line and S2‐CP8, its metastatic subline, using a retroviral short hairpin RNA expression vector. DKK‐1 knockdown resulted in reduced migratory activity of SUIT‐2 in vitro. The in vitro growth rate and Matrigel invasion were also suppressed by DKK‐1 knockdown in S2‐CP8 cells. Collectively, the evidence suggests that, despite of its presumed antagonistic role in Wnt signaling, DKK‐1 may have a role in the aggressiveness of pancreatic carcinoma cells and could, therefore, serve as a novel biomarker of pancreatic cancer.
Finger vein recognition using minutia‐based alignment and local binary pattern‐based feature extraction
Authors: Lee Eui Chul , Lee Hyeon Chang , Park Kang Ryoung 
Published: August 2009

AbstractWith recent increases in security requirements, biometrics such as fingerprints, faces, and irises have been widely used in many recognition applications including door access control, personal authentication for computers, Internet banking, automatic teller machines, and border‐crossing controls. Finger vein recognition uses the unique patterns of finger veins to identify individuals at a high level of accuracy. This article proposes a new finger vein recognition method using minutia‐based alignment and local binary pattern (LBP)‐based feature extraction. Our study makes three novelties compared to previous works. First, we use minutia points such as bifurcation and ending points of the finger vein region for image alignment. Second, instead of using the whole finger vein region, we use several extracted minutia points and a simple affine transform for alignment, which can be performed at fast computational speed. Third, after aligning the finger vein image based on minutia points, we extract a unique finger vein code using a LBP, which reduces false rejection error and thus the equal error rate (EER) significantly. Our resulting EER was 0.081% with a total processing time of 118.6 ms. © 2009 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 19, 179–186, 2009
Detection and segmentation of cervical cell cytoplast and nucleus
Authors: Lin Chuen‐Horng , Chan Yung‐Kuan , Chen Chun‐Chieh 
Published: August 2009

AbstractThis article aims to develop a method for the detection and segmentation of a cytoplast and nucleus from a cervix smear image. First, the technique of equalization method with Gaussian filter is adopted to eliminate noise in the image. Second, a new edge enhancement technique is proposed to work out the coarseness of each pixel, which is later used as a determining characteristic of reinforced object images. A two‐group object enhancement technique is then used to reinforce this object according to rough pixels. Third, the proposed detector enhances the gradients of the edges of the cytoplast and nucleus while suppressing the noise gradients, and then specifies the pixels with higher gradients as possible edge pixels. Finally, it picks out the two longest closed curves constructed by part of the edge pixels. Detection and segmentation performance of the proposed method is later compared with seed region growing feature extraction and level set method using 10 cervix smear images as example. Besides comparing the contour segment of the cytoplast and nucleus obtained by using different methods, we also compare the quality of the segmentation results. Experimental results show that the proposed detector demonstrates an impressive performance. © 2009 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 19, 260–270, 2009
Secure video distribution scheme based on partial encryption
Authors: Lian Shiguo 
Published: August 2009

AbstractIn TV program distribution, the receiver's identification information is embedded into video data in order to trace illegal distributors, and video data are encrypted in order to protect the confidentiality against unauthorized users. In traditional method, the TV program is firstly fingerprinted, then encrypted, and finally transmitted. ForNreceivers,Ntimes of encryption, andNtimes of fingerprinting operations are required, which makes the sender of high loading. In another method, the media content is decrypted and fingerprinted simultaneously by the joint fingerprint embedding and decryption, which is difficult to obtain reasonable performances. In this article, a commutative encryption and fingerprinting scheme is proposed to reduce the sender's loading. In this scheme, the TV program is firstly encrypted, then fingerprinted, and finally transferred. When compared with the traditional method, in the proposed method, only once encryption andNtimes of fingerprinting are required, which reduces the sender's loading greatly. Based on MPEG2 videos, a commutative encryption and fingerprinting scheme is presented, whose performances including security, efficiency, robustness, and imperceptibility are evaluated. Experimental results show that the scheme obtains good performances and is suitable for TV program distribution. © 2009 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 19, 227–235, 2009
Plant leaf identification using Gabor wavelets
Authors: Casanova Dalcimar , de Mesquita Sá Junior Jarbas Joaci , Bruno Odemir Martinez 
Published: August 2009

AbstractThis article presents a novel method of plant classification using Gabor wavelet filters to extract texture filters in a foliar surface. The aim of this promising method is to add to the results obtained by other leaf attributes (such as shape, contour, color, among others), increasing, therefore, the percentage of classification of plant species. To corroborate the efficiency of the technique, an experiment using 20 species from Brazilian flora was done and discussed. The results are also compared with texture Fourier descriptors and cooccurrence matrices. © 2009 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 19, 236–243, 2009
MUC1 is a substrate for γ‐secretase
Authors: Julian JoAnne , Dharmaraj Neeraja , Carson Daniel D. 
Published: August 2009

AbstractUnderstanding the underlying mechanisms by which a normal cell avoids the oncogenic potential of MUC1 signaling requires further definition of the pathways by which the MUC1 cytoplasmic tail is processed in both normal and tumor‐derived cells. In the present study we describe the processing pathway initiated by TACE/ADAM17 cleavage of MUC1. Utilizing the human uterine epithelial cell line, HES, derived from normal endometrium, we show that endogenous full length MUC1 undergoes regulated intramembranous proteolysis mediated by presenillin‐dependent γ‐secretase. Cytokine‐stimulated HES cells exposed to γ‐secretase inhibitors accumulated a membrane‐associated 15 kDa fragment of the MUC1 C‐terminal subunit (CTF15). Inhibitors of TACE/ADAM17‐mediated shedding inhibited accumulation of MUC1‐CTF15 and MUC1 ectodomain release to a similar extent consistent with MUC1‐CTF15 being a product of TACE/ADAM17 action. Reduction of catalytically active γ‐secretase complex by nicastrin siRNA treatment also resulted in CTF15 accumulation. Furthermore, mature nicastrin, the substrate receptor for γ‐secretase, co‐immunoprecipitated with CTF15 in the presence of γ‐secretase inhibitors indicating the formation of CTF15: nicastrin complexes. MUC1‐CTF15 accumulation in response to γ‐secretase inhibition was demonstrated in both normal and tumor‐derived cells from humans and mice indicating that this processing pathway exists in many cell contexts. We did not detect products of MUC1 cleavage by γ‐secretase in the presence of various proteasomal inhibitors indicating that subsequent degradation is either non‐proteasomal or extremely efficient. We suggest that this efficient pathway attenuates potential signaling mediated by cytoplasmic tail fragments. J. Cell. Biochem. 108: 802–815, 2009. © 2009 Wiley‐Liss, Inc.
The influence of innate and pre‐existing immunity on adenovirus therapy
Authors: Zaiss Anne K. , Machado Hidevaldo B. , Herschman Harvey R. 
Published: August 2009

AbstractRecombinant adenovirus serotype 5 (Ad5) vectors have been studied extensively in preclinical gene therapy models and in a range of clinical trials. However, innate immune responses to adenovirus vectors limit effectiveness of Ad5 based therapies. Moreover, extensive pre‐existing Ad5 immunity in human populations will likely limit the clinical utility of adenovirus vectors, unless methods to circumvent neutralizing antibodies that bind virus and block target cell transduction can be developed. Furthermore, memory T cell and humoral responses to Ad5 are associated with increased toxicity, raising safety concerns for therapeutic adenovirus vectors in immunized hosts. Most preclinical studies have been performed in naïve animals; although pre‐existing immunity is among the greatest hurdles for adenovirus therapies, it is also one of the most neglected experimentally. Here we summarize findings using adenovirus vectors in naïve animals, in Ad‐immunized animals and in clinical trials, and review strategies proposed to overcome innate immune responses and pre‐existing immunity. J. Cell. Biochem. 108: 778–790, 2009. © 2009 Wiley‐Liss, Inc.
Critical role of poly(ADP‐ribose) polymerase‐1 in modulating the mode of cell death caused by continuous oxidative stress
Authors: Son Young‐Ok , Kook Sung‐Ho , Jang Yong‐Suk , Shi Xianglin , Lee Jeong‐Chae 
Published: August 2009

AbstractContinuously generated hydrogen peroxide (H2O2) inhibits typical apoptosis and instead initiates a caspase‐independent, apoptosis‐inducing factor (AIF)‐mediated pyknotic cell death. This may be related to H2O2‐mediated DNA damage and subsequent ATP depletion, although the exact mechanisms by which the mode of cell death is decided after H2O2exposure are still unclear. Accumulated evidence and our previous data led us to hypothesize that continuously generated H2O2, not an H2O2bolus, induces severe DNA damage, signaling poly(ADP‐ribose) polymerase‐1 (PARP‐1) activation, ATP depletion, and eventually caspase‐independent cell death. Results from the present study support that H2O2generated continuously by glucose oxidase causes excessive DNA damage and PARP‐1 activation. Blockage of PARP‐1 by a siRNA transfection or by pharmacological inhibitor resulted in the significant inhibition of ATP depletion, loss of mitochondrial membrane potential, nuclear translocation of AIF and endonuclease G, and eventually conversion to caspase‐dependent apoptosis. Overall, the current study demonstrates the different roles of PARP‐1 inhibition in modulation of cell death according to the method of H2O2exposure, that is, continuous generation versus a direct addition. J. Cell. Biochem. 108: 989–997, 2009. © 2009 Wiley‐Liss, Inc.
Characterization of patients discharged from inpatient treatment for burnout: use of psychological characteristics to identify aftercare needs
Authors: Haberthür Annina K. , Elkuch Florina M. , Grosse Holtforth Martin , Hochstrasser Barbara , Soyka Michael 
Published: August 2009

AbstractWith increasing severity, burnout corresponds to a state of significant suffering with impaired social functioning, decreased quality of life, and psychosomatic complaints. The present study investigates (a) how former patients of an inpatient clinic for burnout therapy can be grouped on the basis of their psychological characteristics and (b) whether these groups correspond to different levels of residual symptoms (depression and burnout) and general mental health. Cluster analysis of psychological characteristics was used to form groups. Four specific groups could be identified (functionals, dysfunctionals, straightforward pragmatists, unhappy altruists). This grouping, with subsequent characterization according to symptom levels, provides important information indicating possible needs for aftercare and suggests areas for improvement of previous care. © 2009 Wiley Periodicals, Inc. J Clin Psychol 65: 1–17, 2009.
Rapid recycling of ClC‐2 chloride channels between plasma membrane and endosomes: Role of a tyrosine endocytosis motif in surface retrieval
Authors: Cornejo Isabel , Niemeyer María Isabel , Zúñiga Leandro , Yusef Yamil R. , Sepúlveda Francisco V. , Cid L. Pablo 
Published: August 2009

AbstractClC‐2 chloride channel is present in the brain and some transporting epithelia where its function is poorly understood. We have now demonstrated that the surface channels are rapidly internalised and approximately the 70% of the surface membrane protein recycles after 4‐ to 8‐min internalisation. Endocytosis of ClC‐2 was dependent upon tyrosine 179 located within an endocytic motif. Rapid recycling accompanied by an even faster internalisation could account for the abundant presence of ClC‐2 in intracellular membranous structures. At least a proportion of ClC‐2 resides in lipid rafts. Use of β‐cyclodextrin led to an increase in cell surface channel, but, surprisingly, a decrease in functionally active channels. We suggest that ClC‐2 requires residing in β‐cyclodextrin sensitive clusters with other molecules in order to remain active. Regulation of ClC‐2 trafficking to and within the membrane could be a means of modulating its activity. J. Cell. Physiol. 221: 650–657, 2009. © 2009 Wiley‐Liss, Inc.
Calcium/calmodulin‐dependent protein kinase type IV is a target gene of the Wnt/β‐catenin signaling pathway
Authors: Arrázola Macarena S. , Varela‐Nallar Lorena , Colombres Marcela , Toledo Enrique M. , Cruzat Fernando , Pavez Leonardo , Assar Rodrigo , Aravena Andrés , González Mauricio , Montecino Martín , Maass Alejandro , Martínez Servet , Inestrosa Nibaldo C. 
Published: August 2009

AbstractCalcium/calmodulin‐dependent protein kinase IV (CaMKIV) plays a key role in the regulation of calcium‐dependent gene expression. The expression of CaMKIV and the activation of CREB regulated genes are involved in memory and neuronal survival. We report here that: (a) a bioinformatic analysis of 15,476 promoters of the human genome predicted several Wnt target genes, being CaMKIV a very interesting candidate; (b) CaMKIV promoter contains TCF/LEF transcription motifs similar to those present in Wnt target genes; (c) biochemical studies indicate that lithium and the canonical ligand Wnt‐3a induce CaMKIV mRNA and protein expression levels in rat hippocampal neurons as well as CaMKIV promoter activity; (d) treatment of hippocampal neurons with Wnt‐3a increases the binding of β‐catenin to the CaMKIV promoter: (e) In vivo activation of the Wnt signaling improve spatial memory impairment and restores the expression of CaMKIV in a mice double transgenic model for Alzheimer's disease which shows decreased levels of the kinase. We conclude that CaMKIV is regulated by the Wnt signaling pathway and that its expression could play a role in the neuroprotective function of the Wnt signaling against the Alzheimer's amyloid peptide. J. Cell. Physiol. 221: 658–667, 2009. © 2009 Wiley‐Liss, Inc.