bluuebunny/crossref_metadata_2025_split huggingface dataset:
From Fundamental Interfacial Reaction Kinetics to Macroscopic Current–Voltage Characteristics: Case Study of Solid Acid Fuel Cell Limitations and Possibilities
Authors: Wang Louis S. , Haile Sossina M. 
Published: May 2024

AbstractThe unique properties of solid acid electrolytes, in particular CsH2PO4, are in many ways ideal for fuel cell operation. However, the technology is constrained by high cathode overpotentials. Here a simplified cathode geometry is employed to obtain the fundamental electrochemical parameters (exchange current density and charge transfer coefficient) describing the oxygen reduction reaction (ORR) at the CsH2PO4‐Pt‐gas interface. The parameters are incorporated into a 1D model of the voltage–current characteristics of realistic SAFC cathodes, which reproduced the measured polarization behavior of such cathodes without recourse to fitting adjustable parameters. Following this validation, the model is utilized to evaluate the impact of changes to cathode properties, microstructure, and operating conditions. Of these, the charge transfer coefficient, measured to have a value of ≈0.6 for ORR on Pt in the SAFC cathode environment, is found to have the greatest impact on power output. Nevertheless, even without material modifications, a combination of microstructural and operational modifications are identified with projected performance metrics meeting Department of Energy targets (0.8 V at 300 mA cm−2, and peak power density of 1 W cm−2), albeit at high Pt loadings. However, the analysis indicates that truly meaningful advances will likely necessitate the discovery of alternative ORR catalysts.
Divergent Synthesis of Naphthalenone Spiroindanediones or 4‐Acyl‐1‐naphthols from Aryl Enaminones Featuring Reuse of Dimethylamine
Authors: Yang Chun , Li Bin , Zhang Xinying , Fan Xuesen 
Published: May 2024

AbstractPresented herein is a switchable synthesis of naphthalenone spiroindanediones or 4‐acyl‐1‐naphthols via condition‐controlled divergent cascade reactions of aryl enaminones with diazoindanediones. When the reaction was carried out in the presence of an oxidative additive, naphthalenone spiroindanedione was formed through C(sp2)−H alkylation followed by simultaneous spiroannulation and elimination of dimethylamine. When the reaction was carried out in the presence of an acid additive, on the other hand, 4‐acyl‐1‐naphthol was formed through ring opening of the spiroindanedione moiety attacked by the in situ released dimethylamine. In addition, the reactions are suitable for gram‐scale applications and compatible with a variety of substrates. Moreover, the naphthol products allow structural elaborations and hybridization with a drug, thus further enhancing the value of this developed method.
Discovering farmers’ views of on‐farm precision experimentation
Authors: Tibbs Reagen G. , Boerngen Maria A. 
Published: May 2024

AbstractPrecision agriculture technologies (PATs) have revolutionized agriculture production and provide many benefits to farmers. Among these benefits is the ability to conduct experiments using PATs and collaborate with researchers in a process known as on‐farm precision experimentation (OFPE). OFPE is a citizen‐science approach that fosters relationships and knowledge‐sharing to address challenges of mutual interest. While the literature on precision agriculture is extensive, little research has addressed farmers’ willingness to conduct OFPE with researchers. Interviews with 11 Illinois farmers revealed high adoption rates of PATs. Interest in collaborating with researchers to conduct OFPE was mixed, with farmers identifying clear channels of communication as the most important factor in their decision to collaborate. While additional study is needed among the larger farming community, these initial insights may contribute to the larger effort to encourage greater collaboration between researchers and farmers to find real‐world solutions to agronomic, economic, and environmental challenges.Core Ideas
Precision agriculture technologies (PATs) allow farmers to engage in on‐farm precision experimentation (OFPE).
OFPE fosters farmer and researcher collaboration to help address agronomic challenges.
This study provides initial insight into farmers’ perceptions of and interest in OFPE.
A milder form of NSRP1‐associated neurodevelopmental disorder, caused by a missense variant in the nuclear localization signal
Authors: Neuens Sebastian , Kausar Maiza , Kang Sun‐Kyoung , Soblet Julie , Van Dooren Sonia , Olsen Catharina , Janssen Toon , Caljon Ben , Jun Chang‐Duk , Smits Guillaume , Coppens Sandra , Vilain Catheline 
Published: May 2024

AbstractNuclear Speckle Splicing Regulator Protein 1 (NSRP1) is a splice factor found in nuclear speckles, which are small membrane‐free organelles implicated in epigenetic regulation, chromatin organization, DNA repair, and RNA modification. Bi‐allelic loss‐of‐function variants in NSRP1 have recently been identified in patients suffering from a severe neurodevelopmental disorder, presenting with neurodevelopmental delay, epilepsy, microcephaly, hypotonia, and spastic cerebral palsy. Described patients acquired neither independent walking nor speech and often showed anomalies on cerebral MRI. Here we describe the case of a 14‐year‐old girl with motor and language delay as well as intellectual disability, who presents an ataxic gait but walks without assistance and speaks in short sentences. Whole‐genome sequencing revealed the compound heterozygous NSRP1 variants c.114 + 2T > G and c.1595T > A (p.Val532Glu). Functional validation using HEK293T cells transfected with either wild‐type or mutated GFP‐tagged Nsrp1 suggests that the Val532Glu variant interferes with the function of the nuclear localization signal, and leads to mislocalization of NSRP1 in the cytosol, thus confirming the pathogenicity of the observed variant. This case helps to expand the phenotypic and genetic spectrum associated with pathogenic NSRP1 variants and indicates that this diagnosis should also be suspected in patients with milder phenotypes.
“Brain‐IT”: Exergame training with biofeedback breathing in neurocognitive disorders
Authors: Manser Patrick , de Bruin Eling D. 
Published: May 2024

AbstractINTRODUCTIONThe combination of exergame‐based motor‐cognitive training with resonance breathing guided by heart‐rate variability biofeedback (HRV‐BF) targets various relevant mechanisms of action to alleviate the pathological state in mild neurocognitive disorders (mNCD).METHODSThis randomized controlled trial (RCT) investigated the effectiveness of adding this novel intervention approach to usual care in mNCD. The individualized intervention was delivered via the “Brain‐IT” training concept, which was iteratively co‐designed, tested, and refined with patient and public involvement.RESULTSWe observed statistically significant effects with large effect sizes for global cognitive performance, immediate verbal recall, and delayed verbal recall in favor of the intervention group. Fifty‐five percent of participants showed a clinically relevant improvement in response to training.DISCUSSIONConfirmatory RCTs are warranted to investigate whether the observed improvements in cognitive performance translate to affecting the rates of progression to or onset of dementia and test the implementation of the training in clinical practice.HighlightsWe proposed a novel intervention approach for mild neurocognitive disorders.It combines exergame‐based training with biofeedback‐guided resonance breathing.Our results confirm the effectiveness of this approach.Fifty‐five percent of participants showed a clinically relevant improvement in response to training.
Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation
Authors: Stoltzfus Andrew T. , Ballot Jasper G. , Vignane Thibaut , Li Haoju , Worth Madison M. , Muller Ludovic , Siegler Maxime A. , Kane Maureen A. , Filipovic Milos R. , Goldberg David P. , Michel Sarah L. J. 
Published: May 2024

AbstractThe gasotransmitter hydrogen sulfide (H2S) is thought to be involved in the post‐translational modification of cysteine residues to produce reactive persulfides. A persulfide‐specific chemoselective proteomics approach with mammalian cells has identified a broad range of zinc finger (ZF) proteins as targets of persulfidation. Parallel studies with isolated ZFs show that persulfidation is mediated by ZnII, O2, and H2S, with intermediates involving oxygen‐ and sulfur‐based radicals detected by mass spectrometry and optical spectroscopies. A small molecule ZnII complex exhibits analogous reactivity with H2S and O2, giving a persulfidated product. These data show that ZnII is not just a biological structural element, but also plays a critical role in mediating H2S‐dependent persulfidation. ZF persulfidation appears to be a general post‐translational modification and a possible conduit for H2S signaling. This work has implications for our understanding of H2S‐mediated signaling and the regulation of ZFs in cellular physiology and development.
Development of Ti3C2Tx‐based novel immunosensor for cancer biomarker detection
Authors: Sweety  , Kumar Devendra 
Published: May 2024

In this study, an ultrasensitive label‐free electrochemical immunosensor based on Ti3C2Tx (Mxene) with a 2D‐layered morphology has been proposed for the detection of EpCAM antigen. A hydrothermal method is employed for the synthesis of Ti3C2Tx by using a less toxic exfoliating reagent, NaBF4. The bioelectrode, BSA/anti‐EpCAM/Ti3C2Tx@ITO, has been fabricated by electrophoretic deposition of Ti3C2Tx onto the ITO electrode, followed by an immobilization of EpCAM antibody. Electrochemical response studies reveal that the immunosensor shows a high sensitivity of 29.22 μA fg−1 ml cm−2 and a wide linear range from 0.1 fg/ml to 100 ng/ml, for EpCAM antigen detection. Further, the good stability of this fabricated immunosensor is an additional advantage for EpCAM antigen detection in serum samples.
One‐pot immobilization of formate dehydrogenase on silica nanoparticles based on catechol chemistry with or without polyethyleneimine
Authors: Chong Ruqing , Meng Lingding , Liao Qiyong , Meng Zihui , Liu Wenfang 
Published: May 2024

AbstractConverting carbon dioxide into formic acid with formate dehydrogenase (FDH) as a catalyst is a gentle and eco‐friendly process that not only conserves energy and reduces emissions but transforms waste into valuable resource. Enzyme immobilization is a common strategy for improving the stability and recyclability of enzyme, however, the procedures are usually complicated that include surface modification and binding reactions in order to form reliable linkages. Co‐deposition of polydopamine (PDA) with polyethyleneimine (PEI) is a popular surface modification approach in versatile fields, but their co‐deposition with enzymes is rarely reported. Herein, one‐pot immobilization of FDH on SiO2 nanoparticles based on catechol chemistry was attempted for the first time with or without PEI, and the resultant immobilized enzymes, FDH/PDA/PEI‐SiO2 and FDH/PDA‐SiO2, were characterized, and the effects of the composition and deposition conditions on the catalytic performance were investigated. FDH/PDA‐SiO2 exhibited a higher apparent specific activity than FDH/PDA/PEI‐SiO2, which was separately 0.167 and 0.136 mM/h/mg. The activity recovery rate was 464% and 378% of that of free enzyme. After 5 cycles, their relative activity was 82.3% and 87.4%. This research is significant in presenting a simple and high‐efficient one‐pot immobilization method that can be extended to other kinds of enzymes.
Beyond recognition: Memory, desire and the hellish zone of nonbeing in encounters with otherness
Authors: Swartz Sally 
Published: May 2024

AbstractThis paper is an exploration of possible journeys towards freedom from colonial states of mind. These inner worlds are the distorting forms of self‐observation brought about by living under oppressive conditions, and includes those associated with colonialism itself, and also coloniality, the enduring legacy left behind by colonial regimes. Fanon describes the effect of colonialism on subjectivity as creating “a zone of nonbeing” and suggests freedom from it requires internally “an authentic upheaval.” This paper draws the parallels between Fanon's zone of nonbeing and states of mind untethered from the shackles of colonial definition. These states, akin to reverie are potentially the place from which the quest for a new authenticity of being might be found.
Discovery of potent CSK inhibitors through integrated virtual screening and molecular dynamic simulation
Authors: Chen Roufen , Wang Yuchen , Shen Zheyuan , Ye Chenyi , Guo Yu , Lu Yan , Ding Jianjun , Dong Xiaowu , Xu Donghang , Zheng Xiaoli 
Published: May 2024

AbstractOncogenic overexpression or activation of C‐terminal Src kinase (CSK) has been shown to play an important role in triple‐negative breast cancer (TNBC) progression, including tumor initiation, growth, metastasis, drug resistance. This revelation has pivoted the focus toward CSK as a potential target for novel treatments. However, until now, there are few inhibitors designed to target the CSK protein. Responding to this, our research has implemented a comprehensive virtual screening protocol. By integrating energy‐based screening methods with AI‐driven scoring functions, such as Attentive FP, and employing rigorous rescoring methods like Glide docking and molecular mechanics generalized Born surface area (MM/GBSA), we have systematically sought out inhibitors of CSK. This approach led to the discovery of a compound with a potent CSK inhibitory activity, reflected by an IC50 value of 1.6 nM under a homogeneous time‐resolved fluorescence (HTRF) bioassay. Subsequently, molecule 2 exhibits strong growth inhibition of MD anderson ‐ metastatic breast (MDA‐MB) ‐231, Hs578T, and SUM159 cells, showing a level of growth inhibition comparable to that observed with dasatinib. Treatment with molecule 2 also induced significant G1 phase accumulation and cell apoptosis. Furthermore, we have explored the explicit binding interactions of the compound with CSK using molecular dynamics simulations, providing valuable insights into its mechanism of action.
Data‐efficient and risk‐based pressure sensor placement optimization for water systems
Authors: Chen Thomas Ying‐Jeh , Dryer Joseph , Rathor Ankur , Hoover Greg , Ganjidoost Amin 
Published: May 2024

AbstractDeployment of remote metering infrastructure can help utilities improve pressure management and limit pipe break impacts. Since it is cost‐infeasible to install sensors to fully cover an entire system, a risk‐based approach where devices are deployed in areas of systemic vulnerabilities will maximize the benefit of these limited resources. Previous work on sensor allocations requires well‐calibrated hydraulic models that contain a full asset inventory. This high barrier of data requirement makes it difficult to apply existing methods. In this research, we present a more efficient methodology with lower data requirements. Proxies for system vulnerabilities are generated using historic break records, and GIS inventories are used to derive candidate installation points. The problem is presented as a generalized version of the well‐studied maximal coverage location problem, where locations are selected for optimal coverage of risky areas. The workflow is demonstrated on a small suburban utility in Pennsylvania, USA.
Pharmacokinetics of IMM‐H012 in rats using ultra‐performance liquid chromatography‐tandem mass spectrometry
Authors: Wu Shujuan , Wu Jialei , Lin Longquan , Jiang Rongbin , Wang Xianqin , Wen Congcong , Zhu Xia‐yin 
Published: May 2024

AbstractThe present study examined the pharmacokinetics of IMM‐H012 in rat plasma, utilizing ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS). Internal standard cilostazol was employed, and plasma samples were processed using acetonitrile precipitation. A mobile phase (acetonitrile–0.1% formic acid in water) with gradient elution was used to achieve chromatographic separation using a UPLC BEH C18 column. In multiple reaction monitoring mode, electrospray ionization MS/MS was utilized in positive ionization mode. Based on findings, the lower limit of quantification was 2 ng/mL, and the linearity of IMM‐H012 in rat plasma was found to be acceptable within the range of 2–2000 ng/mL (R2 > 0.995). The intra‐day and inter‐day precision relative standard deviation was less than 14% of IMM‐H012 in rat plasma. The matrix effect was within the range of 102%–107%, and the accuracy ranged from 92% to 113%. Pharmacokinetics of IMM‐H012 in rats after oral administration were successfully studied using UPLC‐MS/MS.
Evaluation of quality consistency between dispensing granules and traditional decoction of Bombyx batryticatus based on peptidomics and in silico simulations
Authors: Huang Yayang , Liu Yaxiong , Xiao Xiaotong , Guo Dong , Zeng Wenjie , Luo Zhuoya 
Published: May 2024

AbstractThe application of traditional Chinese medicine dispensing granules is becoming increasingly prevalent. However, the consistency of dispensing granules with traditional decoction remains controversial. In this study, the consistency of peptide composition and pharmacodynamics between dispensing granules and traditional decoction of Bombyx batryticatus (BB) were assessed. A peptidomics method based on LC–tandem mass spectrometry technology was used to evaluate peptide composition similarity between BB traditional decoction and dispensing granules. The results revealed notable differences in peptide sequences between the two dosage forms, with only 8.55% of peptides shared between them. To evaluate the potential pharmacodynamic effects of the two dosage forms on epilepsy, virtual screening was used to identify potential active peptides, including blood–brain barrier permeability, toxicity prediction, and molecular docking. BB traditional decoction demonstrated a higher number and greater abundance of potential active peptides than BB dispensing granules, suggesting that BB traditional decoction may have a more favorable effect in treating epilepsy compared with BB dispensing granules. Moreover, molecular docking and molecular dynamics simulation studies confirmed the mechanism of action of active peptides to γ‐aminobutyric acid transporter 1 (GAT‐1). This study provides a scientific basis for the evaluation of quality consistency between BB traditional decoction and dispensing granules.
Exosome‐related lncRNA score: A value‐based individual treatment strategy for predicting the response to immunotherapy in clear cell renal cell carcinoma
Authors: Yang Zhan , Zhang Xiaoting , Zhan Ning , Lin Lining , Zhang Jingyu , Peng Lianjie , Qiu Tao , Luo Yaxian , Liu Chundi , Pan Chaoran , Hu Junhao , Ye Yifan , Jiang Zilong , Liu Xinyu , Sun Mouyuan , Zhang Yan 
Published: May 2024

AbstractBackgroundExosomes play a crucial role in intercellular communication in clear cell renal cell carcinoma (ccRCC), while the long non‐coding RNAs (lncRNAs) are implicated in tumorigenesis and progression.AimsThe purpose of this study is to construction a exosomes‐related lncRNA score and a ceRNA network to predict the response to immunotherapy and potential targeted drug in ccRCC.MethodsData of ccRCC patients were obtained from the TCGA database. Pearson correlation analysis was used to identify eExosomes‐related lncRNAs (ERLRs) from Top10 exosomes‐related genes that have been screened. The entire cohort was randomly divided into a training cohort and a validation cohort in equal scale. LASSO regression and multivariate cox regression was used to construct the ERLRs‐based score. Differences in clinicopathological characteristics, immune microenvironment, immune checkpoints, and drug susceptibility between the high‐ and low‐risk groups were also investigated. Finally, the relevant ceRNA network was constructed by machine learning to analyze their potential targets in immunotherapy and drug use of ccRCC patients.ResultsA score consisting of 4ERLRs was identified, and patients with higher ERLRs‐based score tended to have a worse prognosis than those with lower ERLRs‐based score. ROC curves and multivariate Cox regression analysis demonstrated that the score could be considered as a risk factor for prognosis in both training and validation cohorts. Moreover, patients with high scores are predisposed to experience poor overall survival, a larger prevalence of advanced stage (III‐IV), a greater tumor mutational burden, a higher infiltration of immunosuppressive cells, and a greater likelihood of responding favorably to immunotherapy. The importance of EMX2OS was determined by mechanical learning, and the ceRNA network was constructed, and EMX2OS may be a potential therapeutic target, possibly exerting its function through the EMX2OS/hsa‐miR‐31‐5p/TLN2 axis.ConclusionsBased on machine learning, a novel ERLRs‐based score was constructed for predicting the survival of ccRCC patients. The ERLRs‐based score is a promising potential independent prognostic factor that is closely correlated with the immune microenvironment and clinicopathological characteristics. Meanwhile, we screened out key lncRNAEMX2OS and identified the EMX2OS/hsa‐miR‐31‐5p/TLN2 axis, which may provide new clues for the targeted therapy of ccRCC.
Long‐COVID symptoms improved after MDMA and psilocybin therapy: A case report
Authors: Chopra Harman , Furnish Tim , Verduzco‐Gutierrez Monica , Jevotovsky David S. , Castellanos Joel 
Published: May 2024

Key Clinical MessageLong‐COVID syndrome lacks effective holistic treatment options. We present a case of a 41‐year‐old fully vaccinated female with Long‐COVID syndrome who obtained significant symptomatic relief after self‐medicating with psilocybin and MDMA.AbstractLong‐COVID, a syndrome persisting after the acute phase of coronavirus disease 2019 (COVID‐19), lacks effective holistic treatment options. We present a case of a 41‐year‐old fully vaccinated female with Long‐COVID syndrome who obtained significant symptomatic relief by self‐prescribing psilocybin and MDMA. Future research is needed to assess safety and efficacy.
Selective Synthesis of Formyl‐2‐Furancarboxylic Acid via Enhanced Adsorption of 5‐Hydroxymethylfurfural on Composite Catalysts
Authors: Wang Di , Lu Xingyu , Xu Haiyan , Dou Jing , Zhang Xuefei , Xie Zailai , Qi Wei 
Published: May 2024

Abstract5‐Hydroxymethylfurfural (HMF) is a widely used biomass platform chemical that plays a crucial role in bridging biomass and fossil resources. Electrocatalytic oxidation of HMF provides an efficient way to obtain high‐value‐added biomass‐derived chemicals, among which the intermediate product 5‐formyl‐2‐furan carboxylic acid (FFCA) has attracted considerable attention. However, the weak adsorption ability of monometallic nickel oxide (NiOx) to HMF in neutral electrolyte restricts its further development, resulting in low HMF conversion and FFCA yield. In this study, we successfully constructed a novel nickel oxide‐platinum oxide hybrid catalyst supported on carbon felt (NiOx‐PtOx/CF), which exhibits an optimized adsorption ability of HMF, leading to the outstanding FFCA yield up to 77 % in the neutral media. The high activity of the NiOx‐PtOx/CF catalyst can be attributed to the redistribution of the electrons and the optimization of the electronic structure on the Ni active site due to the introduction of PtOx on NiOx nanosheets. This study offers valuable insights for the design of efficient multicomponent electrocatalysts for electrocatalytic biomass refinery systems.