bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translatomics reveals the role of dietary calcium addition in regulating muscle fat deposition in pigs
Authors: Yu Jingsu , Li Xiangling , Qi Xinyu , Ding Zhaoxuan , Su Songtao , Yu Lin , Zhou Lei , Li Yixing 
Published: May 2024

AbstractIntramuscular fat (IMF) in pork holds significant importance for economic performance within the pig industry and dietary calcium supplementation enhances the accumulation of intramuscular fat. Additionally, calcium ions inhibit translation and reduce protein synthesis. However, the mechanism by which calcium regulates IMF deposition in muscle through translation remains largely unknown. In this study, we compared the ribosome profiles of the longissimus dorsi muscles of Duroc $$\times$$
×
Landrace $$\times$$
×
Large white pigs from the normal calcium (NC) group or calcium supplement (HC) group by Ribo-seq, and RNA-seq. By integrating multiple-omics analysis, we further discovered 437 genes that were transcriptionally unchanged but translationally altered and these genes were significantly enriched in the oxidative phosphorylation signaling pathway. Furthermore, experimental data showed that inhibiting the expression of COX10 and mtND4L increased triglyceride accumulation in C2C12 cells, providing new targets for intramuscular fat deposition. Finally, this work links dietary calcium, translation regulation and IMF deposition, providing a new strategy for both meat quality and economic performance within the pig industry.
Nanomaterials functionalized acidic ionic organosilica as highly active catalyst in the selective synthesis of benzimidazole via dehydrogenative coupling of diamines and alcohols
Authors: Rajabi Fatemeh , Feiz Afsaneh 
Published: May 2024

AbstractAn acidic tungstate-based zwitterionic organosilica drived simple self-condensation of tungstic acid and zwitterionic organosilane (PMO-IL-WO42−), was remarkably demonstrated to be highly efficient and environmentally friendly catalyst for directly selective synthesis of benzimidazoles from benzyl alcohols under atmpshpheric air pressure and without any additional oxidant. The one-pot synthesis of benzimidazoles from benzyl alcohols and 1,2-phenylenediamine was efficiently achieved via direct dehydrogenative reaction using a low amount of recoverable PMO-IL-WO42− nanocatalyst in water under ambient conditions with a conversion efficiency of more than 90%. Enhancements in yield and selectivity of benzimidazole formation were observed when water was utilized as the solvent. Furthermore, the PMO-IL-WO42− nanocatalyst exhibited exceptional stability, demonstrating the ability to be effortlessly separated and reused for at least eight reaction cycles without any noticeable loss in activity or product selectivity. This method supports an eco-friendly atom economy and provides a sustainable approach to accessing benzimidazoles directly from benzyl alcohols under mild conditions, demonstrating its potential for practical applications in organic synthesis.
Survival after partial heart transplantation in a piglet model
Authors: Medina Cathlyn K. , Moya-Mendez Mary E. , Aykut Berk , Jeffs Sydney , Kang Lillian , Evans Amy , Parker Lauren E. , Miller Stephen G. , Helke Kristi L. , Overbey Douglas M. , Turek Joseph W. , Rajab Taufiek Konrad 
Published: May 2024

AbstractPartial heart transplantation (PHT) is a novel surgical approach that involves transplantation of only the part of the heart containing a valve. The rationale for this approach is to deliver growing heart valve implants that reduce the need for future re-operations in children. However, prior to clinical application of this approach, it was important to assess it in a preclinical model. To investigate PHT short-term outcomes and safety, we performed PHT in a piglet model. Yorkshire piglets (n = 14) were used for PHT of the pulmonary valve. Donor and recipient pairs were matched based on blood types. The piglets underwent PHT at an average age of 44 days (range 34–53). Post-operatively, the piglets were monitored for a period of two months. Of the 7 recipient piglets, one mortality occurred secondary to anesthesia complications while undergoing a routine echocardiogram on post-operative day 19. All piglets had appropriate weight gain and laboratory findings throughout the post-operative period indicating a general state of good health and rehabilitation after undergoing PHT. We conclude that PHT has good short-term survival in the swine model. PHT appears to be safe for clinical application.
A deep learning approach for electric motor fault diagnosis based on modified InceptionV3
Authors: Xu Lifu , Teoh Soo Siang , Ibrahim Haidi 
Published: May 2024

AbstractElectric motors are essential equipment widely employed in various sectors. However, factors such as prolonged operation, environmental conditions, and inadequate maintenance make electric motors prone to various failures. In this study, we propose a thermography-based motor fault detection method based on InceptionV3 model. To enhance the detection accuracy, we apply Contrast Limited Adaptive Histogram Equalization (CLAHE) to the input images. Furthermore, we improved the performance of the InceptionV3 by integrating a Squeeze-and-Excitation (SE) channel attention mechanism. The proposed model was tested using a dataset containing 369 thermal images of an electric motor with 11 types of faults. Image augmentation was employed to increase the data size and the evaluation was conducted using fivefold cross validation. Experimental results indicate that the proposed model can achieve accuracy, precision, recall, and F1 score of 98.82%, 98.93%, 98.82%, and 98.87%, respectively. Additionally, by freezing the fully connected layers of the InceptionV3 model for feature extraction and training a Support Vector Machines (SVM) to perform classification, it is able to achieve 100% detection rate across all four evaluation metrics. This research contributes to the field of industrial motor fault diagnosis. By incorporating deep learning techniques based on InceptionV3 and SE channel attention mechanism with a traditional classifier, the proposed method can accurately classify different motor faults.
Advanced magnetic resonance imaging detects altered placental development in pregnancies affected by congenital heart disease
Authors: Cromb Daniel , Slator Paddy J. , Hall Megan , Price Anthony , Alexander Daniel C. , Counsell Serena J. , Hutter Jana 
Published: May 2024

AbstractCongenital heart disease (CHD) is the most common congenital malformation and is associated with adverse neurodevelopmental outcomes. The placenta is crucial for healthy fetal development and placental development is altered in pregnancy when the fetus has CHD. This study utilized advanced combined diffusion-relaxation MRI and a data-driven analysis technique to test the hypothesis that placental microstructure and perfusion are altered in CHD-affected pregnancies. 48 participants (36 controls, 12 CHD) underwent 67 MRI scans (50 control, 17 CHD). Significant differences in the weighting of two independent placental and uterine-wall tissue components were identified between the CHD and control groups (both pFDR < 0.001), with changes most evident after 30 weeks gestation. A significant trend over gestation in weighting for a third independent tissue component was also observed in the CHD cohort (R = 0.50, pFDR = 0.04), but not in controls. These findings add to existing evidence that placental development is altered in CHD. The results may reflect alterations in placental perfusion or the changes in fetal-placental flow, villous structure and maturation that occur in CHD. Further research is needed to validate and better understand these findings and to understand the relationship between placental development, CHD, and its neurodevelopmental implications.
The mediating role of ICT learning confidence and technostress between executive functions and digital skills
Authors: Bali Cintia , Feher Zsuzsanna , Arato Nikolett , Kiss Botond Laszlo , Labadi Beatrix , Zsido Andras Norbert 
Published: May 2024

AbstractHaving good digital skills is essential today, yet little is known about the cognitive factors that influence the development of these skills. Given the importance of executive functions (EFs) in adapting to environmental changes and acquiring skills, EFs might contribute to acquiring digital skills too. EFs might also influence people’s approach toward ICTs and affect digital skills through emotional variables. Therefore, here, we tested whether cognitive control and flexibility are connected to computer and smartphone skills through emotional factors (learning confidence, stress, and attitude). A total of 269 participants (56 males, M = 30 years) filled out our survey which included questionnaires on demographic variables (age, education, and socioeconomic status), ICT motivation, digital skills, EFs, and technology-related emotional factors. EFs were also investigated through two performance-based measures. We used generalized linear models and structural equation modeling to test the associations between these variables. The results showed that smartphone skills were positively associated with self-reported cognitive flexibility through ICT learning confidence and technology-induced stress. Self-reported cognitive control and age were connected to smartphone skills directly. Self-reported cognitive flexibility was also associated with computer skills through ICT learning confidence. In addition, performance-based cognitive control and the level of education were directly linked to computer skills. These results may provide guidance to support digital skills and create digital skill training.
Clinical outcomes of colorectal neoplasm with positive resection margin after endoscopic submucosal dissection
Authors: Oh Hyung-Hoon , Kim Je-Seong , Lim Jae-Woong , Lim Chae-June , Seo Young-Eun , You Ga-Ram , Im Chan-Muk , Kim Ki-Hyun , Kim Dong-Hyun , Kim Hyun-Soo , Joo Young-Eun 
Published: May 2024

AbstractA positive resection margin after colorectal endoscopic submucosal dissection (ESD) is associated with an increased risk of recurrence. We aimed to identify the clinical significance of positive resection margins in colorectal neoplasms after ESD. We reviewed 632 patients who had en bloc colorectal ESD at two hospitals between 2015 and 2020. The recurrence rates and presence of residual tumor after surgery were evaluated. The rate of additional surgery after ESD and recurrence rate were significantly higher in patients with incomplete resection (n = 75) compared to patients with complete resection (n = 557). When focusing solely on non-invasive lesions, no significant differences in recurrence rates were observed between the groups with complete and incomplete resection (0.2% vs. 1.9%, p = 0.057). Among 84 patients with submucosal invasive carcinoma, 39 patients underwent additional surgery due to non-curative resection. Positive vertical margin and lymphovascular invasion were associated with residual tumor. Lymphovascular invasion was associated with lymph node metastasis. However, no residual tumor nor lymph node metastases were found in patients with only one unfavorable histological factor. In conclusion, a positive resection margin in non-invasive colorectal lesions, did not significantly impact the recurrence rate. Also, in T1 colorectal cancer with a positive vertical resection margin, salvage surgery can be considered in selected patients with additional risk factors.
Characteristics and mechanism of local scour reduction around spur dike using the collar in clear water
Authors: Qi Hongliang , Wang Jie , Zou Wen , Luo Wenjun , Tian Weiping , Li Jiachun 
Published: May 2024

AbstractTo reduce the local scour around the spur dike, the U-shaped collar is proposed in this study. The influence of the collar's length, width, and porosity on the local scour reduction in clear water is studied by model tests and numerical simulations. Experimental studies show that the collar has a significant effect on reducing the local scour. The location of the maximum scour depth of the spur dike moves downstream. The width of the collar has the greatest impact on the reduction effect among the three selected factors, followed by the porosity and the length. Local scour reduction efficiency of the collar can reach 56.9%. Based on the regression analysis of the range and variety, a formula for predicting the reduction effect around the spur dike is put forward, and the deviation between the values by formula and that in experiments are within ± 4%. The characteristics of the flow field around the spur dike under constant conditions with a collar are studied via numerical simulation. The numerical simulation results show that compared to the case without collar, the flow velocities around the spur dike in cases with permeable collar and solid collar reduced by 45% and 25%, respectively, and the shear stresses reduced by 20% and 28.6%, respectively. The results of this study can provide a reference for local scour reduction using the solid collar or collar made of permeable materials such as gabions.
Detection of atherosclerotic plaques with HDL-like porphyrin nanoparticles using an intravascular dual-modality optical coherence tomography and fluorescence system
Authors: Chen Rouyan , Sandeman Lauren , Nankivell Victoria , Tan Joanne T. M. , Rashidi Mohammad , Psaltis Peter J. , Zheng Gang , Bursill Christina , McLaughlin Robert A. , Li Jiawen 
Published: May 2024

AbstractAtherosclerosis is the build-up of fatty plaques within blood vessel walls, which can occlude the vessels and cause strokes or heart attacks. It gives rise to both structural and biomolecular changes in the vessel walls. Current single-modality imaging techniques each measure one of these two aspects but fail to provide insight into the combined changes. To address this, our team has developed a dual-modality imaging system which combines optical coherence tomography (OCT) and fluorescence imaging that is optimized for a porphyrin lipid nanoparticle that emits fluorescence and targets atherosclerotic plaques. Atherosclerosis-prone apolipoprotein (Apo)e-/- mice were fed a high cholesterol diet to promote plaque development in descending thoracic aortas. Following infusion of porphyrin lipid nanoparticles in atherosclerotic mice, the fiber-optic probe was inserted into the aorta for imaging, and we were able to robustly detect a porphyrin lipid-specific fluorescence signal that was not present in saline-infused control mice. We observed that the nanoparticle fluorescence colocalized in areas of CD68+ macrophages. These results demonstrate that our system can detect the fluorescence from nanoparticles, providing complementary biological information to the structural information obtained from simultaneously acquired OCT.
An absorptive coding metasurface for ultra-wideband radar cross-section reduction
Authors: Lin Baoqin , Huang Wenzhun , Guo Jianxin , Wang Zuliang , Si Kaibo , Ye Hongjun 
Published: May 2024

AbstractIn this paper, an absorptive coding metasurface (ACM) is proposed for ultra-wideband radar cross section (RCS) reduction, the design process is presented in detail, in which a lossy polarization conversion metasurface (PCM) is proposed at first. The lossy PCM is an anisotropic resistive structure with both polarization conversion and absorption performances, so that its co-polarization reflection coefficients under u- and v-polarized incidences can be kept at less than − 10 dB in magnitude in the frequency range from 7.5 to 45.2 GHz. Though the magnitude of the cross-polarization reflection coefficient cannot be very small only due to the absorption, its phase will be changed by nearly 180° when the unit-cell structure of the lossy PCM is rotated by 90°. Thus, the lossy PCM can be used as one of the two types of lossy coding elements for an ACM when its unit-cell structure is rotated by 90° or not. Based on the lossy PCM, an ACM is proposed. The simulation and experimental results show that the ACM has an excellent RCS reduction performance under arbitrary polarized incidence, it can achieve effective RCS reduction under normal incidence in the ultra-wide frequency band from 7.4 to 45.5 GHz with a ratio bandwidth (fH/fL) of 6.15:1; moreover, an ultra-wideband RCS reduction can still be achieved when the incident angle is increased to 45°, which indicates that the ACM has good stealth performance under the detection of various radars working in X, Ku, K and Ka bands, it is very practical.
Analytical performance evaluation of the Mindray enzymatic assay for hemoglobin A1c measurement
Authors: Li Mingyang , Wu Xiongjun , Xie Weijie , Zeng Yu , Wang Hui , Chen Han , Xu Anping , Liu Helu , Ji Ling 
Published: May 2024

AbstractHemoglobin A1c (HbA1c) plays a crucial role in diabetes management. We aimed to evaluate the analytical performance of a new enzymatic method kit for HbA1c measurement. The performance of the enzymatic method, including precision, accuracy, and linearity, was evaluated. Moreover, the interference effect from conventional interferents, Hb derivatives, Hb variants, and common drugs were assessed. In addition, the agreement of HbA1c results was compared between enzymatic methods, cation-exchange high-performance liquid chromatography (HPLC), and immunoassays. The intra-assay, between-assay, and total precision of HbA1c were all lower than 2%. HbA1c showed good linearity within the range of 3.96–20.23%. The enzymatic assay yielded results consistent with the external quality control samples, with a bias of less than ± 6% from the target values. The enzymatic method showed no interference from bilirubin, intralipid, vitamin C, Hb derivatives, common Hb variants, as well as antipyretic analgesics and hypoglycemic drugs. The HbA1c results of the enzymatic assay showed good agreement and accuracy compared to those obtained from the HPLC method and the immunoassay. The enzymatic method kit performed on the BS-600M chemistry analyzer is a reliable and robust method for measuring HbA1c. It is suitable for routine practice in clinical chemistry laboratories.
PDO influenced interdecadal summer precipitation change over East China in mid-18th century
Authors: Chen Gebanruo , Li Xiangyu , Xu Zhiqing , Liu Yong , Zhang Zhongshi , Shao Shiyu , Gao Jing 
Published: May 2024

AbstractThere have been few case studies of the relationship between the Pacific Decadal Oscillation (PDO) and the East Asian climate before the pre-industrial era with limited anthropogenic impacts. Using the CESM Last Millennium Ensemble (CESM-LME) simulation with reconstruction evidence, we showed that there was an interdecadal transition of the summer precipitation in East China, with the pattern of “southern flooding and northern drought” in the mid-18th century. The interdecadal transition was influenced by PDO, as suggested by both the reconstruction evidence and simulation. Corresponding to the positive PDO phase change, the East Asia-Pacific pattern teleconnection wave train propagated northward and modulated the circulation and precipitation in East China, together with the southward movement of the East Asian westerly jet. The volcanic double or clustered eruptions are thought to have played a crucial role on the shift of the PDO phase and the decadal summer climate change over East China during the mid-18th century. Incorporating volcanic activity in a reasonable manner would likely improve decadal simulations of East Asian climate in the past and predictions in the future.
Uncovering kinesin dynamics in neurites with MINFLUX
Authors: Wirth Jan Otto , Schentarra Eva-Maria , Scheiderer Lukas , Macarrón-Palacios Victor , Tarnawski Miroslaw , Hell Stefan W. 
Published: May 2024

AbstractNeurons grow neurites of several tens of micrometers in length, necessitating active transport from the cell body by motor proteins. By tracking fluorophores as minimally invasive labels, MINFLUX is able to quantify the motion of those proteins with nanometer/millisecond resolution. Here we study the substeps of a truncated kinesin-1 mutant in primary rat hippocampal neurons, which have so far been mainly observed on polymerized microtubules deposited onto glass coverslips. A gentle fixation protocol largely maintains the structure and surface modifications of the microtubules in the cell. By analyzing the time between the substeps, we identify the ATP-binding state of kinesin-1 and observe the associated rotation of the kinesin-1 head in neurites. We also observed kinesin-1 switching microtubules mid-walk, highlighting the potential of MINFLUX to study the details of active cellular transport.
TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation
Authors: Martí-Andrés Pablo , Finamor Isabela , Torres-Cuevas Isabel , Pérez Salvador , Rius-Pérez Sergio , Colino-Lage Hildegard , Guerrero-Gómez David , Morato Esperanza , Marina Anabel , Michalska Patrycja , León Rafael , Cheng Qing , Jurányi Eszter Petra , Borbényi-Galambos Klaudia , Millán Iván , Nagy Péter , Miranda-Vizuete Antonio , Schmidt Edward E , Martínez-Ruiz Antonio , Arnér Elias SJ , Sastre Juan 
Published: May 2024

AbstractIt has remained unknown how cells reduce cystine taken up from the extracellular space, which is a required step for further utilization of cysteine in key processes such as protein or glutathione synthesis. Here, we show that the thioredoxin-related protein of 14 kDa (TRP14, encoded by TXNDC17) is the rate-limiting enzyme for intracellular cystine reduction. When TRP14 is genetically knocked out, cysteine synthesis through the transsulfuration pathway becomes the major source of cysteine in human cells, and knockout of both pathways becomes lethal in C. elegans subjected to proteotoxic stress. TRP14 can also reduce cysteinyl moieties on proteins, rescuing their activities as here shown with cysteinylated peroxiredoxin 2. Txndc17 knockout mice were, surprisingly, protected in an acute pancreatitis model, concomitant with activation of Nrf2-driven antioxidant pathways and upregulation of transsulfuration. We conclude that TRP14 is the evolutionarily conserved enzyme principally responsible for intracellular cystine reduction in C. elegans, mice, and humans.
The Effects of Interactive Context on Acoustic Characteristics of Speech in People With Dysarthria: A Preliminary Study
Authors: Krajewski Elizabeth , Lee Jimin , Viswanathan Navin , Olmstead Anne , Simmons Zachary 
Published: May 2024

Purpose:
The current study compared temporal and spectral acoustic contrast between vowel segments produced by speakers with dysarthria across three speech tasks—interactive, solo habitual, and solo clear.

Method:
Nine speakers with dysarthria secondary to amyotrophic lateral sclerosis participated in the study. Each speaker was paired with a typical interlocutor over videoconferencing software. The speakers produced the vowels /i, ɪ, ɛ, æ/ in /h/−vowel−/d/ words. For the solo tasks, speakers read the stimuli aloud in both their habitual and clear speaking styles. For the interactive task, speakers produced a target stimulus for their interlocutor to select among the four possibilities. We measured the duration difference between long and short vowels, as well as the F1/F2 Euclidean distance between adjacent vowels, and also determined how well the vowels could be classified based on their acoustic characteristics.

Results:
Temporal contrast between long and short vowels was higher in the interactive task than in both solo tasks. Spectral distance between adjacent vowel pairs was also higher for some pairs in the interactive task than the habitual speech task. Finally, vowel classification accuracy was highest in the interactive task.

Conclusions:
Overall, we found evidence that individuals with dysarthria produced vowels with greater acoustic contrast in structured interactions than they did in solo tasks. Furthermore, the speech adjustments they made to the vowel segments differed from those observed in solo speech.