bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experiences of co-producing person-centred and cohesive clinical pathways in the national system for knowledge-based management in Swedish healthcare: a qualitative study
Authors: Määttä Sylvia , Petersson Christina , Gäre Boel Andersson , Henriks Göran , Ånfors Henrik , Lundberg Christin , Nilsagård Ylva 
Published: June 2024

Abstract
Background
When the 21 Swedish county councils decided to collaborate in the creation of a national system for knowledge-based management, patient participation was mandatory. Patient and next-of-kin representatives (PR) co-produced person-centred and cohesive clinical pathways together with healthcare professionals (HPR). Research on co-production in healthcare at the national level is scarce. The aim of this study is to explore experiences of patient participation from the perspectives of both PRs and HPRs when co-producing clinical pathways within the Swedish nationwide healthcare system for knowledge-based management.

Methods
A qualitative study was conducted. A strategic sample of nine PRs and eight HPRs were interviewed individually between August 2022 and January 2023 using a semi-structured interview guide. We analysed data using an inductive content analysis.

Results
Three main categories were identified: (1) Finding appropriate patient representativeness; (2) Working methods that facilitate a patient perspective; and (3) Influence of the patient perspective in the clinical pathways.

Conclusions
The study demonstrates the importance of patient and next-of-kin participation in the construction of clinical pathways at the national level. The results provide a platform for further research on patient participation on the national level and add to studies on if and how patient participation on this level has an impact on how the clinical pathways are put into practice at the micro level, and the support provided at the meso level. The study contributes to the growing body of literature studying patient participation and co-production.

Trial registration
Region Örebro County ID 276,940. An advisory opinion was obtained from the Swedish Ethical Review Authority (2021-05899-01).
Time-of-flight MRA of intracranial vessels at 7 T
Authors: Cosottini Mirco , Calzoni Tommaso , Lazzarotti Guido Andrea , Grigolini Alessandro , Bosco Paolo , Cecchi Paolo , Tosetti Michela , Biagi Laura , Donatelli Graziella 
Published: June 2024

Abstract
Background
Three-dimensional time-of-flight magnetic resonance angiography (TOF-MRA) is a largely adopted non-invasive technique for assessing cerebrovascular diseases. We aimed to optimize the 7-T TOF-MRA acquisition protocol, confirm that it outperforms conventional 3-T TOF-MRA, and compare 7-T TOF-MRA with digital subtraction angiography (DSA) in patients with different vascular pathologies.

Methods
Seven-tesla TOF-MRA sequences with different spatial resolutions acquired in four healthy subjects were compared with 3-T TOF-MRA for signal-to-noise and contrast-to-noise ratios as well as using a qualitative scale for vessel visibility and the quantitative Canny algorithm. Four patients with cerebrovascular disease (primary arteritis of the central nervous system, saccular aneurism, arteriovenous malformation, and dural arteriovenous fistula) underwent optimized 7-T TOF-MRA and DSA as reference. Images were compared visually and using the complex-wavelet structural similarity index.

Results
Contrast-to-noise ratio was higher at 7 T (4.5 ± 0.8 (mean ± standard deviation)) than at 3 T (2.7 ± 0.9). The mean quality score for all intracranial vessels was higher at 7 T (2.89) than at 3 T (2.28). Angiogram quality demonstrated a better vessel border detection at 7 T than at 3 T (44,166 versus 28,720 pixels). Of 32 parameters used for diagnosing cerebrovascular diseases on DSA, 27 (84%) were detected on 7-T TOF-MRA; the similarity index ranged from 0.52 (dural arteriovenous fistula) to 0.90 (saccular aneurysm).

Conclusions
Seven-tesla TOF-MRA outperformed conventional 3-T TOF-MRA in evaluating intracranial vessels and exhibited an excellent image quality when compared to DSA. Seven-tesla TOF-MRA might improve the non-invasive diagnostic approach to several cerebrovascular diseases.

Relevance statement
An optimized TOF-MRA sequence at 7 T outperforms 3-T TOF-MRA, opening perspectives to its clinical use for noninvasive diagnosis of paradigmatic pathologies of intracranial vessels.

Key points
• An optimized 7-T TOF-MRA protocol was selected for comparison with clinical 3-T TOF-MRA for assessing intracranial vessels.
• Seven-tesla TOF-MRA outperformed 3-T TOF-MRA in both quantitative and qualitative evaluation.
• Seven-tesla TOF-MRA is comparable to DSA for the diagnosis and characterization of intracranial vascular pathologies.

Graphical Abstract
Experimental characterization of Grewia Ferruginea fiber reinforced polyester matrix composite materials
Authors: Seyoum Dejen , Asratie Aychew , Gnanadurai Robinson , Gzate Yewondwosen 
Published: June 2024

AbstractResearchers have recently altered their focus and have become more interested in natural fiber-reinforced polymer composites because they are more ecologically friendly and environmentally conscious than synthetic fiber-reinforced polymer composites. Among the best sources of natural fiber, Grewia ferruginea, sometimes known locally as Lenquata, is a source of natural fiber from other plant fibers. The goal of this study was to create polyester matrix composites reinforced with short Grewia ferruginea plant fibers measuring 10, 20, and 30 mm. The fibers were extracted using the traditional water-retting method and chemically treated with 5% NaOH. The findings indicated that the average tensile strength of a single fiber from Grewia ferruginea plants is 214 MPa, with a density of 1.11 g/cm3. Furthermore, the composite, which was created with a fiber length of 10 mm, fiber weight ratio of 25 %, and polyester matrix composite of 75 %, exhibited superior performance since it is stronger than any combination that was used to create the composite in this investigation, with 18.3 MPa tensile and 35.2 MPa flexural strength.
TAK-242 (Resatorvid) inhibits proinflammatory cytokine production through the inhibition of NF-κB signaling pathway in fibroblast-like synoviocytes in osteoarthritis patients
Authors: Khomeijani-Farahani Mohammadreza , karami Jafar , Farhadi Elham , Soltani Samaneh , Delbandi Ali-Akbar , Shekarabi Mehdi , Tahmasebi Mohammad Naghi , Vaziri Arash Sharafat , Jamshidi Ahmadreza , Mahmoudi Mahdi , Akhlaghi Masoomeh 
Published: June 2024

Abstract
Background
Fibroblast-like synoviocytes (FLSs) are involved in osteoarthritis (OA) pathogenesis through pro-inflammatory cytokine production. TAK-242, a TLR4 blocker, has been found to have a significant impact on the gene expression profile of pro-inflammatory cytokines such as IL1-β, IL-6, TNF-α, and TLR4, as well as the phosphorylation of Ikβα, a regulator of the NF-κB signaling pathway, in OA-FLSs. This study aims to investigate this effect because TLR4 plays a crucial role in inflammatory responses.

Materials and methods
Ten OA patients’ synovial tissues were acquired, and isolated FLSs were cultured in DMEM in order to assess the effectiveness of TAK-242. The treated FLSs with TAK-242 and Lipopolysaccharides (LPS) were analyzed for the mRNA expression level of IL1-β, IL-6, TNF-α, and TLR4 levels by Real-Time PCR. Besides, we used western blot to assess the protein levels of Ikβα and pIkβα.

Results
The results represented that TAK-242 effectively suppressed the gene expression of inflammatory cytokines IL1-β, IL-6, TNF-α, and TLR4 which were overexpressed upon LPS treatment. Additionally, TAK-242 inhibited the phosphorylation of Ikβα which was increased by LPS treatment.

Conclusion
According to our results, TAK-242 shows promising inhibitory effects on TLR4-mediated inflammatory responses in OA-FLSs by targeting the NF-κB pathway. TLR4 inhibitors, such as TAK-242, may be useful therapeutic agents to reduce inflammation and its associated complications in OA patients, since traditional and biological treatments may not be adequate for all of them.
Associations of community knowledge, perceptions, and practices related to zoonotic disease with sociodemographic factors in and around Chiro Town, Eastern Ethiopia: a cross-sectional study
Authors: Abrahim Abdulaziz , Bekele Bantayehu , Tahir Muhidin , Ahmed Sali , Ahmedin Lencho 
Published: June 2024

Abstract
Background
Zoonoses are infectious diseases that are transmitted from animals to humans. Studying the knowledge, perceptions and practices of communities related to zoonoses and the associated risk factors is crucial for effective control and prevention. This study aimed to assess the knowledge, perceptions, and practices of respondents on zoonoses and the associated risk factors in and around Chiro town, Ethiopia. Zoonotic diseases, such as rabies, anthrax, bovine tuberculosis, and brucellosis, pose a direct threat to health and livelihoods in the communities where they occur. These diseases emerge due to a combination of human-animal interactions, migration, and contact with wildlife and their respective parasites and vectors. Hence, recognizing residents’ perceptions, knowledge, and practices is crucial for effectively minimizing risks.

Methods
A cross-sectional study was conducted from January 2020 to July 2021 in and around Chiro town using a pretested close-ended questionnaire. A total of 350 respondents were selected using simple random sampling methods. The questionnaire included information on the sociodemographic status of the respondents and questions concerning the knowledge, perceptions, and practices of the participants regarding the selected zoonotic diseases. The associations of knowledge, perceptions, and practices related to zoonoses with zoonotic risk factors were analysed using chi-square tests.

Results
The study revealed that 82.9% of the respondents had knowledge of bovine tuberculosis, followed by knowledge of rabies (80%), knowledge of anthrax (45.1%), and knowledge of brucellosis (24.3%). Males had greater knowledge of bovine tuberculosis (84.8%), followed by rabies (79.8%) and anthrax (48.6%), while females had greater knowledge of brucellosis (23.6%). The most cited source of information was radio (68%). Most respondents mentioned the outbreaks of rabies (62.5%), bovine tuberculosis (53.2%), anthrax (35.6%), and brucellosis (15.7%). Respondents with higher educational levels and urban residents had more knowledge of zoonoses. More than 75% of respondents had a good perception of the transmission of zoonotic disease from animals, and the practice of consuming raw milk or raw/undercooked meat and sharing the same house with animals was high.

Conclusion
The majority of respondents reported that they had knowledge of bovine tuberculosis and rabies, but lower knowledge and perceptions were reported for anthrax and brucellosis. These findings illustrate the need for collaboration among animal, human and environmental health offices in one health approach to prevent and control zoonotic disease.
Identification and molecular mapping of resistance genes for adult-plant resistance to stripe rust in spring wheat germplasm line PI660076
Authors: Ren Runsheng , Zhou Jianian , Wang Yuqi , Wang Lirong , Zheng Xiaochen , Li Xin , Yang Suizhuang , Zhou Xinli , Zhang Pingping 
Published: June 2024

AbstractWheat is one of the major food crops worldwide. Stripe rust can cause a great loss of wheat yield, especially when the disease is prevalent. Chemical control not only causes the loss of resistance to stripe rust but also has a serious impact on the human body and environment. Therefore, the most economical measure to control wheat stripe rust is to cultivate resistant varieties. Rapid variation of stripe rust races often leads to rapid "loss" of resistance to stripe rust disease; therefore, breeders and researchers have to continuously explore new stripe rust resistance genes to provide new resistance sources for the rapid variation of stripe rust races. Previous studies have confirmed that PI660076, a spring wheat line, showed stripe rust resistance under natural conditions at the adult stage, which has great value in breeding programs. In this study, a recombinant inbred lines (RIL) population was constructed by crossing the wheat line PI660076 with the stripe rust-susceptible line AvS. Genotyping of the population was performed using a wheat 15 K SNP array. Three QTLs were identified using phenotypic data over four years across three environments. The resistance type of each QTL was determined by inoculating the RIL lines with single and homozygous QTL during the seedling and adult stages under controlled conditions. The all-stage resistance (ASR) QTL, QYr076.jaas-2A (flanked by SNP marker AX-11048464 and Kompetitive Allele-Specific PCR (KASP) marker KASP_4940) explained 7.13–16.58% and 6.95–7.25% of infection type (IT) and disease severity (DS), respectively. The adult-plant resistance (APR) resistance QTL, QYr076.jaas-4D.1 (flanked by KASP marker KASP_0795 and SNP marker AX-111567243,) explained 6.85–12.70% and 7.94–17.26% of IT and DS, respectively. The APR resistance QTL, QYr076.jaas-4D.2 flanked by KASP markers KASP_9130 and KASP_6535, explained 7.97–39.19% and 8.77–20.55% of the phenotypic variation in IT and DS, respectively. All the three QTLs are likely to be new. The obtained results lay a foundation for further utilization of the stripe rust-resistant line PI660076, as well as for fine mapping and molecular marker-assisted selection breeding.
Graphical Abstract
Tricyclic microwave-assisted synthesis of gold nanoparticles for biomedical applications: combatting multidrug-resistant bacteria and fungus
Authors: Al Azzam Sarah , Ullah Zabih , Azmi Sarfuddin , Islam Mozaffarul , Ahmad Ishtiaque , Hussain Mohd Kamil 
Published: June 2024

Abstract
Background
Rising global mortality due to antibiotic-resistant pathogens necessitates novel antibacterial and antifungal agents. This study focuses on synthesizing gold nanoparticles (GNPs) via tricyclic microwave irradiation (TMI) to combat Multi-Drug-Resistant Bacteria and Fungus. The demand for sustainable synthesis methods has led to the exploration of TMI for GNP production.

Results
Characterization demonstrates consistent, uniform, and dispersed GNPs with trigonal and hexagonal shapes. GNPs sized 20–55 nm exhibit superior antibacterial and antifungal activity, particularly against drug-resistant Gram-positive bacteria. Notably, GNPs display consistent efficacy against drug-resistant fungus and demonstrate potential for broad-spectrum antimicrobial applications.

Conclusion
TMI-synthesized GNPs, characterized by their favorable physical properties and size-dependent efficacy, show promise as effective agents against drug-resistant pathogens. Their ability to combat Gram-positive bacteria, Gram-negative bacteria, and drug-resistant fungus positions them as valuable tools in biomedical sciences. By addressing the urgent need for novel antimicrobial agents, TMI-synthesized GNPs offer a sustainable solution to the escalating global health challenge of antibiotic resistance.
Effect of Switching the Histamine-1 Receptor Antagonist Clemastine to Cetirizine in Paclitaxel Premedication Regimens: The H1-Switch Study
Authors: Malmberg Ruben , van Doorn Leni , Cox Juul M. , Daloul Alaa , Ettafahi Halima , Oomen-de Hoop Esther , Zietse Michiel , Bos Monique E.M.M. , Koch Birgit C.P. , van Leeuwen Roelof W.F. 
Published: June 2024

PURPOSE Premedication, including a histamine-1 receptor (H1) antagonist, is recommended to all patients treated with paclitaxel chemotherapy to reduce the incidence of hypersensitivity reactions (HSRs). However, the scientific basis for this premedication is not robust, which provides opportunities for optimization. Substitution of intravenously administered first-generation H1 antagonist for orally administered second-generation H1 antagonist could reduce side effects, and improve efficiency and sustainability. This study investigates the efficacy and safety of substituting intravenous clemastine for oral cetirizine as prophylaxis for paclitaxel-induced HSRs. METHODS This single-center, prospective, noninferiority study compares a historic cohort receiving a premedication regimen with intravenous clemastine to a prospective cohort receiving oral cetirizine. Primary end point of the study is HSR grade ≥3. The difference in incidence was calculated together with the 90% CI. We determined that the two-sided 90% CI of HSR grade ≥3 incidence in the oral cetirizine cohort should not be more than 4% higher (ie, the noninferiority margin) compared with the intravenous clemastine cohort. RESULTS Two hundred and twelve patients were included in the oral cetirizine cohort (June 2022 and May 2023) and 183 in the intravenous clemastine cohort. HSR grade ≥3 incidence was 1.6% (n = 3) in the intravenous clemastine cohort and 0.5% (n = 1) in the oral cetirizine cohort, resulting in a difference of –1.2% (90% CI, –3.4 to 1.1). CONCLUSION Premedication containing oral cetirizine is as safe as premedication containing intravenous clemastine in preventing paclitaxel-induced HSR grade ≥3. These findings could contribute to optimization of care for patients and improve efficiency and sustainability.
Implementation of and Systems-Level Barriers to Guideline-Driven Germline Genetic Evaluation in the Care of Patients With Myelodysplastic Syndrome and Acute Myeloid Leukemia
Authors: Banaszak Lauren G. , Cabral Paloma L. , Smith-Simmer Kelcy , Hassan Ayesha , Brunner Matthew , Fallon Michael , Shoger Kyle , Lovrien Lauren , Golner Danielle , Zurbriggen Luke , Mattison Ryan , Gahvari Zhubin , Hall Aric , Nadiminti Kalyan , Reinig Erica , Churpek Jane E. 
Published: June 2024

PURPOSE Knowledge of an inherited predisposition to myelodysplastic syndrome (MDS) and AML has important clinical implications for treatment decisions, surveillance, and care of at-risk relatives. National Comprehensive Cancer Network (NCCN) guidelines recently incorporated recommendations for germline genetic evaluation of patients with MDS/AML on the basis of personal and family history features, but the practicality of implementing these recommendations has not been studied. METHODS A hereditary hematology quality improvement (QI) committee was formed to implement these guidelines in a prospective cohort of patients diagnosed with MDS/AML. Referral for germline genetic testing was recommended for patients meeting NCCN guideline criteria. Referral patterns and genetic evaluation outcomes were compared with a historical cohort of patients with MDS/AML. Barriers to evaluation were identified. RESULTS Of the 90 patients with MDS/AML evaluated by the QI committee, 59 (66%) met criteria for germline evaluation. Implementation of the QI committee led to more referrals for germline evaluation in accordance with NCCN guidelines (31% v 14%, P = .03). However, the majority of those meeting criteria were never referred due to high medical acuity or being deceased or in hospice at the time of QI committee recommendations. Despite this, two (17%) of the 12 patients undergoing genetic testing were diagnosed with a hereditary myeloid malignancy syndrome. CONCLUSION Current NCCN guidelines resulted in two thirds of patients with MDS/AML meeting criteria for germline evaluation. A hereditary hematology-focused QI committee aided initial implementation and modestly improved NCCN guideline adherence. However, the high morbidity and mortality and prolonged inpatient stays associated with MDS/AML challenged traditional outpatient genetic counseling models. Further improvements in guideline adherence require innovating new models of genetic counseling and testing for this patient population.
RASopathies – what they reveal about RAS/MAPK signaling in skeletal muscle development
Authors: Rauen Katherine A. , Tidyman William E. 
Published: June 2024

ABSTRACT
RASopathies are rare developmental genetic syndromes caused by germline pathogenic variants in genes that encode components of the RAS/mitogen-activated protein kinase (MAPK) signal transduction pathway. Although the incidence of each RASopathy syndrome is rare, collectively, they represent one of the largest groups of multiple congenital anomaly syndromes and have severe developmental consequences. Here, we review our understanding of how RAS/MAPK dysregulation in RASopathies impacts skeletal muscle development and the importance of RAS/MAPK pathway regulation for embryonic myogenesis. We also discuss the complex interactions of this pathway with other intracellular signaling pathways in the regulation of skeletal muscle development and growth, and the opportunities that RASopathy animal models provide for exploring the use of pathway inhibitors, typically used for cancer treatment, to correct the unique skeletal myopathy caused by the dysregulation of this pathway.
Ranking Decomposition for the Discrete Ordered Median Problem
Authors: Cherkesly Marilène , Contardo Claudio , Gruson Matthieu 
Published: June 2024

Given a set [Formula: see text] of size n, a nonnegative, integer-valued distance matrix D of dimensions [Formula: see text], an integer [Formula: see text] and an integer-valued weight vector [Formula: see text], the discrete ordered median problem (DOMP) consists of selecting a subset [Formula: see text] of exactly p points from [Formula: see text] (also referred to as the centers) so as to: 1) assign each point in [Formula: see text] to its closest center in [Formula: see text]; 2) rank the resulting distances (between every point and its center) from smallest to largest in a sorted vector that we denote [Formula: see text]; 3) minimize the scalar product [Formula: see text]. The DOMP generalizes several classical location problems such as the p-center, the p-median and the obnoxious median problem. We introduce an exact branch-and-bound algorithm to solve the DOMP. This branch-and-bound decouples the ranking attribute of the problem to form a series of simpler subproblems which are solved using innovative binary search methods. We consider several acceleration techniques such as warm-starts, primal heuristics, variable fixing, and symmetry breaking. We perform a thorough computational analysis and show that the proposed method is competitive against several MIP models from the scientific literature. We also comment on the limitations of our method and propose avenues of future research. History: Accepted by Andrea Lodi, Area Editor for Design & Analysis of Algorithms—Discrete. Funding: This work was supported by the Natural Sciences and Engineering Research Council of Canada [Grants 2017-06106, 2020-06311, and 2021-03327]. Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2023.0059 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2023.0059 ). The complete IJOC Software and Data Repository is available at https://informsjoc.github.io/ .