bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coexistence of Hashimoto's thyroiditis with papillary thyroid carcinoma: The influence of lymph node metastasis
Authors: Kim Sang Soo , Lee Byung Joo , Lee Jin Choon , Kim Seong‐Jang , Jeon Yun Kyung , Kim Mi Ra , Huh Jeong Eun , Mok Ji Young , Kim Bo Hyun , Kim Yong Ki , Kim In Joo 
Published: November 2010

AbstractBackgroundThe aim of this study was to evaluate the influence of coexistent Hashimoto's thyroiditis with papillary thyroid carcinoma on lymph node metastasis.MethodsThe coexistent Hashimoto's thyroiditis group was defined by the presence of thyroid autoantibodies or pathologic confirmation. The control group did not have autoantibodies, disease, or ultrasound findings of Hashimoto's thyroiditis.ResultsFor the Hashimoto's thyroiditis group, only central lymph node metastasis had a lower frequency and the mean number of central metastatic lymph nodes was lower than in the control group after adjustment. With regard to lateral lymph node involvement, there was no significant difference between the 2 groups. The multivariate analysis showed a negative association between the coexistence of Hashimoto's thyroiditis and central lymph node metastasis.ConclusionsCoexisting Hashimoto's thyroiditis in patients with papillary thyroid carcinoma was identified as a negative independent predictive factor for central lymph node metastasis. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Significant correlation of peptidyl‐prolyl isomerase overexpression in merkel cell carcinoma with overall survival of patients
Authors: Lill Claudia , Schneider Sven , Pammer Johannes , Loewe Robert , Gedlicka Wilhelm , Houben Roland , Heiduschka Gregor , Brunner Markus , Thurnher Dietmar 
Published: November 2010

AbstractBackgroundAn overexpression of PIN1, the peptidyl‐prolyl cis‐trans isomerase, might cause cell cycle arrest and growth inhibition by binding to the p53 protein, a process leading to p53 stabilization. The rationale of this retrospective analysis was to evaluate the expression pattern of PIN1 in Merkel cell carcinomas (MCCs) and its suitability as a prognostic factor.MethodsSamples of 27 MCCs were immunhistochemically stained for PIN1 expression and correlated with overall and disease‐free survival of patients.ResultsAll samples expressed PIN1. We showed a significantly better overall survival in patients with an overexpression of PIN1 than in patients with a weak PIN1 expression (p= .031), but expression was not significant for disease‐free survival (p= .821). The 5‐year overall survival rate was 14.4% in patients with weak and 50.9% in patients with overexpression of PIN1.ConclusionsPIN1 seems to be a prognostic factor for a better overall survival rate of patients with MCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Stair‐step flap for secondary lower lip revision after lip and cheek composite defects reconstruction
Authors: Fujiwara Takashi , Chen Chien‐Chang , Shih Hsiang‐Shun , Nebres Rico P. , Jeng Seng‐Feng , Kuo Yur‐Ren 
Published: November 2010

AbstractBackgroundFree flap reconstruction is a common procedure for through‐and‐through lip and cheek complex defects after cancer ablation. However, oral incompetence with unacceptable bulky flap appearance invariably occurs and needs a secondary revisionary procedure. The purpose of this article was to present our method to improve the oral competence and cosmetic appearance by the stair‐step technique of flap revision.MethodsFourteen patients who had undergone flap folding reconstruction of the lip and cheek complex defect resulting in oral incontinence were involved in this study. Thirteen patients had been treated with an anterolateral thigh flap and 1 patient had been treated with a fibular osteocutaneous flap. This revisionary stair‐step technique was combined with simultaneous liposuction of the bulky flap in all cases.ResultsAdequate oral continence and good contour was achieved in 12 patients. The remaining 2 patients still had mild drooling, which was managed successfully with a second stair‐step flap.ConclusionThis technique is a valuable option for revision of lower lip deformity. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Comparison of morbidity between sentinel node biopsy and elective neck dissection for treatment of the n0 neck in patients with oral squamous cell carcinoma
Authors: Murer Karin , Huber Gerhard F. , Haile Sarah R. , Stoeckli Sandro J. 
Published: November 2010

AbstractBackgroundSentinel node biopsy (SNB) has been proposed for staging of the cN0 neck in early oral/oropharyngeal squamous cell carcinomas (SCC). Because SNB is a minimally invasive procedure, it is thought to be associated with less morbidity than elective neck dissection.MethodsSixty‐two consecutive patients were included from 2000 to 2009. Two groups were analyzed consisting of 33 patients after SNB and 29 after elective neck dissection. Subjective impairment and functional shoulder status were assessed with the Neck Dissection Impairment Index (NDII) questionnaire and the modified individual relative Constant Score. Postoperative complications were retrieved from the clinical charts.ResultsThe investigated scores were significantly better in the SNB group. All postoperative complications occurred in the elective neck dissection group.ConclusionSNB is associated with significantly less postoperative morbidity and better shoulder function than elective neck dissection. This supports our opinion that patients with nodal negative early SCC of the oral cavity should be offered SNB. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Oral squamous cell carcinoma and a clinically negative neck: The value of follow‐up
Authors: Wensing Bart M. , Merkx Matthias A. W. , Krabbe Paul F. M. , Marres Henri A. M. , Van den Hoogen Frank J. A. 
Published: November 2010

AbstractBackgroundIn squamous cell carcinoma of the oral cavity (SCCOC), regular follow‐up comprises 5 years of prescheduled visits, irrespective of tumor stage/classification and/or treatment. We analyzed our standard treatment and follow‐up protocol in patients with a preoperative clinically negative neck (cN0) in SCCOC.MethodsThis is a retrospective chart analysis. Inventarization of treatment, occult metastatic spread, and follow‐up were performed.ResultsIn all, 197 patients were included. The occult metastatic rate was 24%. Eighty‐three percent of recurrent disease presented within 2 years. Fifty‐three percent of the patients with recurrent disease visited their physician outside prescheduled control visits.ConclusionsUltrasound‐guided fine‐needle aspiration cytology currently is 1 of the most reliable staging techniques in cN0 SCCOC. Regular follow‐up could perhaps be limited from 5 to 2 years of prescheduled follow‐up visits. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Regulation of mammalian muscle type 6‐phosphofructo‐1‐kinase and its implication for the control of the metabolism
Authors: Sola‐Penna Mauro , Da Silva Daniel , Coelho Wagner S. , Marinho‐Carvalho Monica M. , Zancan Patricia 
Published: November 2010

AbstractPhosphofructokinase (PFK) is a major regulatory glycolytic enzyme and is considered to be the pacemaker of glycolysis. This enzyme presents a puzzling regulatory mechanism that is modulated by a large variety of metabolites, drugs, and intracellular proteins. To date, the mammalian enzyme structure has not yet been resolved. However, it is known that PFK undergoes an intricate oligomerization process, shifting among monomers, dimers, tetramers, and more complex oligomeric structures. The equilibrium between PFK dimers and tetramers is directly correlated with the enzyme regulation, because the dimer exhibits very low catalytic activity, whereas the tetramer is fully active. Several PFK ligands modulate the enzyme, favoring the formation of its dimers or tetramers. The present review integrates recent findings regarding the regulatory aspects of muscle type PFK and discusses their relation to the control of metabolism. © IUBMB IUBMB Life, 62(11): 791–796, 2010.
Functional nonequivalence of transketolase active centers
Authors: Kochetov German A. , Sevostyanova Irina A. 
Published: November 2010

AbstractTransketolase (TK, EC 2.2.1.1), the key enzyme of the non‐oxidative branch of pentose phosphate pathway of hydrocarbon transformation, plays an important role in a system of substrate rearrangement between pentose shunt and glycolysis, acting as a reversible link between the two metabolic pathways. In addition, it supplies precursors for biosyntheses of nucleotides, aromatic amino acids, and vitamins. In plants, the enzyme plays a central role in the Calvin cycle. TK catalyzes interconversion of sugar phosphates. Thiamine diphosphate (TDP) and bivalent cations serve as its cofactors. Being a typical TDP‐dependent enzyme, TK is the least complex representative of this group of enzymes, and this accounts for its use as a model in studies of their structure and mechanism of action. TK is readily crystallized, this being the reason why the first crystal X‐ray structure analysis of TDP‐dependent enzymes was performed with a TK sample. Both the general structure of TK and the structures of its active centers have been studied in detail. In this article, we review experimental evidence of functional nonequivalence of the two active centers of TK, which are known to be identical by crystal X‐ray structure analysis. © IUBMB IUBMB Life, 62(11): 797–802, 2010.
Increased female fertility in aquaporin 8‐deficient mice
Authors: Su Weiheng , Qiao Ying , Yi Fei , Guan Xingang , Zhang Di , Zhang Shuzhi , Hao Feng , Xiao Yinghong , Zhang Hongguo , Guo Lei , Yang Longfei , Feng Xuechao , Ma Tonghui 
Published: November 2010

AbstractAquaporin‐8 (AQP8) is a water channel expressed extensively in male and female reproductive systems. But its physiological functions are largely unknown. In the present study, we first found significantly increased number of offspring delivered by AQP8−/− mothers compared with wild‐type mothers in cross‐mating experiments. Comparison of ovulation in the two genotypes demonstrated that AQP8−/− ovaries released more oocytes (9.5 ± 1.9 vs. 7.1 ± 2.1 in normal ovulation and 37.8 ± 6.7 vs. 27.9 ± 5.7 in superovulation). Histological analysis showed increased number of corpus luteums in mature AQP8−/− ovaries, suggesting increased maturation and ovulation of follicles. By RT‐PCR, western blot and immunohistochemistry analyses, we determined the expression of AQP8 in mouse ovarian granulosa cells. Granulosa cells isolated from AQP8−/− mice showed 45% of decreased membrane water permeability than wild‐type mice. As the atresia of ovarian follicles is primarily due to apoptosis of granulosa cells, we analyzed the apoptosis of isolated granulosa cells from wild‐type and AQP8−/− mice. The results indicated significantly lower apoptosis rate in AQP8−/− granulosa cells (21.3 ± 3.6% vs. 32.6 ± 4.3% in AQP8+/+ granulosa cells). Taken together, we conclude that AQP8 deficiency increases the number of mature follicles by reducing the apoptosis of granulosa cells, thus increasing the fertility of female mice. This discovery may offer new insight of improving female fertility by reducing granulosa cell apoptosis through AQP8 inhibition. © IUBMB IUBMB Life, 62(11): 852–857, 2010.
Mechanical properties of cross‐linked collagen meshes after human adipose derived stromal cells seeding
Authors: Ochoa Ignacio , Peña Estefania , Andreu Enrique J. , Pérez‐Ilzarbe Maitane , Robles Jose E. , Alcaine Clara , López Tania , Prósper Felipe , Doblaré Manuel 
Published: November 2010

AbstractThe main goal of this study was to evaluate the potential of collagen meshes derived from porcine dermis as scaffolds for repairing pelvic organ prolapses. Mechanical properties of collagen meshes with different cross‐linking percentages before and after Adipose Derived Stromal Cells (ADSC) seeding were studied as well as the cell‐scaffold interaction. Uniaxial tensile tests of the collagen meshes with three different cross‐linking percentages (full‐, partial‐, and noncross‐linked) were carried out along orthogonal directions. Their mechanical properties were studied with the same tests before and after seeding with human derived adipose stem cells (ADSC) after 1 and 7 days. Histological analyses were performed to determine adhesion and proliferation of ADSC. Significant differences in mechanical properties of the unseeded meshes were observed between each orthogonal direction independently of the cross‐linking percentage. A better cell adhesion rate was observed in the cross‐linked meshes. An increase in the mechanical properties after cell seeding was observed with a direct relation with the degree of cross‐linking. All meshes analyzed showed a marked anisotropy that should be taken into account during the surgical procedure. The cross‐linking treatment increased cell adhesion and the mechanical properties of the collagen meshes after seeding. These results suggest that the mechanical properties of this type of collagen mesh could be useful as scaffolds for repair of pelvic organ prolapse. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Suppression of HIV‐1 transcriptional elongation by a DING phosphatase
Authors: Darbinian Nune , Gomberg Rebeccah , Mullen Loriann , Garcia Samantha , White Martyn K. , Khalili Kamel , Amini Shohreh 
Published: November 2010

AbstractHIV‐1 gene transcription is controlled by the cooperation of viral and host factors which bind to specific DNA sequences within the viral promoter spanning the long terminal repeat (LTR). Previously we showed that the St. John's Wort DING phosphatase, p27SJ, suppresses HIV‐1 gene transcription by binding to the viral protein Tat and preventing its nuclear import. Here, we describe the inhibitory effect of p27SJ on the phosphorylation of the C‐terminal domain (CTD) of RNA polymerase II (RNAPII). This inhibition leads to the suppression of the association of RNAPII with the LTR. Inhibition of binding of RNAPII to LTR by p27SJ resulted in the suppression of LTR transcription elongation and a decrease in LTR transcriptional activity. Another form of the St. John's Wort DING phosphatase, p38SJ, also suppressed binding of RNAPII to the LTR, reduced transcription elongation and was even more powerful than p27SJ in inhibiting the transcriptional activity of the LTR. Our data suggest a possible mechanism by which the p27SJ/p38SJ DING phosphatase can regulate HIV‐1 LTR expression by inhibiting phosphorylation of the CTD of RNAPII and suppressing LTR transcription elongation. J. Cell. Biochem. 112: 225–232, 2011. © 2010 Wiley‐Liss, Inc.
Spin‐driven activation of dioxygen in various metalloenzymes and their inspired models
Authors: De La Lande Aurelien , Salahub Dennis R. , Maddaluno Jacques , Scemama Anthony , Pilme Julien , Parisel Olivier , Gerard Helene , Caffarel Michel , Piquemal Jean‐Philip 
Published: November 2010

AbstractAlthough potentially powerful, molecular oxygen is an inert oxidant due to the triplet nature of its ground state. Therefore, many enzymesse various metal cations (M) to produce singlet active species MnO2. In this communication we investigate the topology of the Electron Localization Function (ELF) within five biomimetic complexes which are representative of the strategies followed by metalloenzymes to activate O2. Thanks to its coupling to the constrained DFT methods the ELF analysis reveals the tight connection between the spin state of the adduct and the spatial organization of the oxygen lone pairs. We suggest that enzymes could resort to spin state control to tune the regioselectivity of substrate oxidations. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
On easy implementation of a variant of the replica exchange with solute tempering in GROMACS
Authors: Terakawa Tsuyoshi , Kameda Tomoshi , Takada Shoji 
Published: November 2010

AbstractTo reduce the number of replicas required in the conventional replica exchange method for huge systems, recently the replica exchange with solute tempering (REST) method was proposed. Here we showed that a variant of REST realized by rescaling the force‐field parameters can be performed with GROMACS 4 without changing the code. We tested the variant REST for alanine dipeptide and an N‐terminal peptide from p53 confirming its performance nearly equal to the original REST. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Density functional study of structural, electronic, and optical properties of small bimetallic ruthenium‐copper clusters
Authors: Karagiannis Efstathios E. , Kefalidis Christos E. , Petrakopoulou Ioanna , Tsipis Constantinos A. 
Published: November 2010

AbstractThe structural, electronic, bonding, magnetic, and optical properties of bimetallic [CunRum]+/0/− (n + m ≤ 3; n, m = 0–3) clusters were computed in the framework of the density functional theory (DFT) and time‐dependent DFT (TD‐DFT) using the full‐range PBE0 nonlocal hybrid GGA functional combined with the Def2‐QZVPP basis sets. Several low‐lying states have been investigated and the stability of the ground state spinomers was estimated with respect to all possible fragmentation schemes. Molecular orbital and population analysis schemes along with computed electronic parameters illustrated the details of the bonding mechanisms in the [CunRum]+/0/− clusters. The TD‐DFT computed UV–visible absorption spectra of the bimetallic clusters have been fully analyzed and assignments of all principal electronic transitions were made and interpreted in terms of contribution from specific molecular orbital excitations. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Fast calculation of DNMR spectra on CUDA‐enabled graphics card
Authors: Szalay Zsófia , Rohonczy János 
Published: November 2010

AbstractDuring the past few years, general‐purpose graphics processing units (GPGPUs) have become rather popular in the high performance computing community. In this study, we present an implementation of the simulation of dynamic nuclear magnetic resonance (DNMR) spectra. The algorithm is based on the kinetic Monte Carlo method and therefore can benefit from the multithreaded architecture of the GPGPU. By careful optimization of the algorithm a 30–100‐fold speed increase could be achieved. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Implementation of CBT for youth affected by the World Trade Center disaster: Matching need to treatment intensity and reducing trauma symptoms
Authors:   CATS Consortium
Published: November 2010

AbstractAn implementation study of cognitive–behavioral therapies (CBT) was conducted for traumatized youth in a postdisaster context. Headed by the New York State Office of Mental Health, the study targeted youth (N = 306) ages 5–21 affected by the World Trade Center disaster. They received either trauma‐specific CBT or brief CBT skills depending upon the severity of trauma symptoms. Clinicians were trained to deliver these interventions and received monthly consultation. A regression discontinuity design was used to assess optimal strategies for matching need to service intensity. At 6‐months postbaseline, both groups had improved. Rate of change was similar despite differences in severity of need. The implications for the implementation of evidence‐based treatments postdisaster are discussed.
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B
Authors: Dai Tianhe , Miletić Nemanja , Loos Katja , Elbahri Mady , Abetz Volker 
Published: November 2010

AbstractPANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal‐B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal‐B‐immobilized PANGMA nanomats were assayed and compared with the free Cal‐B. The observed Cal‐B loading on these nanomats is up to ≈50 mg · g−1, and their hydrolytic activity is up to ≈2 500 nmol · min−1 · mg−1, much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal‐B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal‐B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. magnified image
Radiation Induced Double Grafting of Polybutadiene Olygomers and Styrene onto Silica: Characterization of the Materials and Mechanistic Studies
Authors: Dondi Daniele , Palamini Claudia , Buttafava Armando , Faucitano Antonio , Galinetto Pietro , Nahmias Marco , Giannini Luca , Lostritto Angela 
Published: November 2010

AbstractIonizing radiations from a 60‐Co source have been employed for inducing radical reactions finally leading to the grafting of unsaturated olygomers and vinyl monomers onto the surface and micropores of precipitated silica Zeosil 1165 (150–170 m2/g). The target was to obtain modified silica with enhanced polar compatibility with respect to polybutadiene and styrene‐butadiene copolymers rubber matrices and suited to favour the formation of chemically bound rubber in a vulcanization process. The latter property was expected to follow from the characteristics of free radical reactivity arising from the unsaturations present in the organic coating. Three classes of modified silica have been prepared: A)silica coated with polybutadiene olygomers with Mn in the range 1000–5000 and having a different relative content of 1,4‐cis, 1,4‐trans and vinyl double bonds 1,2; B)Silica coated with polybutadiene olygomers further modified by subsequent grafting of styrene monomer. The type A samples were obtained by γ irradiation under vacuum of silica samples pre‐impregnated with the oligomers in the dose range up to 30–200 kGy. Type B samples were obtained via three different steps as outlined in the scheme below: magnified imageThe characterization of the modified silica was carried on by elemental and thermogravimetric analysis, FTIR (diffuse reflectance), Raman spectroscopy, inverse gas chromatography (IGC), CP/MAS 29‐Si NMR, granulometry and TEM microscopy.
Poly(lactic acid) Synthesis in Solution Polymerization
Authors: Marques Dina A.S. , Jarmelo Susana , G. Baptista Cristina M.S. , Gil M.H. 
Published: November 2010

AbstractThe step‐growth polymerization of L‐lactic acid in solution was studied in this work. In order to attain a polymer with high molecular weight, the water formed during the polymerization must be continuously removed. The use of organic solvents with high boiling point, drying agents and reduced pressure led to poly(lactic acid) (PLA) with high molecular weight, directly from the monomer. Tin (II) chloride dihydrate, SnCl2.2H2O, was the best of the catalysts tested as it allowed achieving PLA with a molecular weight close to 80 000 g.mol−1. However, the stereoregurarity control is a severe problem in PLA synthesis by step‐growth due to transesterification reactions, which lead to an inversion of the conformation and a decrease of the optical purity of the polymer. Specific rotation measurements were used in this work and showed to be a powerful technique to evaluate the racemization extent. The thermal stability of the PLA samples was evaluated by DSC which exhibits a thermal behaviour similar to the commercial Polylactide.
Living Polymerization of 1‐Trimethylsilyl‐1‐propyne and 4‐Methyl‐2‐pentyne by NbCl5‐Based Catalysts
Authors: Sultanov E. Yu. , Khotimskiy V. S. 
Published: November 2010

AbstractSequential homopolymerization of disubstituted acetylenes 1‐trimethylsilyl‐1‐propyne and 4‐methyl‐2‐pentyne by NbCl5–Ph3SiH was investigated and the main evidence of living polymerization, namely, continuation of chain propagation after addition of a new portion of monomer was observed. AB and BA type block copolymers of 1‐trimethylsilyl‐1‐propyne and 4‐methyl‐2‐pentyne were synthesized by sequential polymerization of these monomers in presence of NbCl5–based catalytic systems.