bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring actors' collaborations and involvement in the Namibian learner pregnancy policy
Authors: Shiningayamwe Dorthea Nanghali Etuwete , Takeuchi Shinichi 
Published: April 2024

IntroductionThe Namibia Education Sector Policy on the Prevention and Management of Learner Pregnancy (ESPPMLP) aims to reduce the cases of learners falling pregnant and increase learner-parent completion of education. The ESPPMLP addresses the fact that learner pregnancy and school drop-out are collaborative efforts between parents, the community, schools, and non-governmental organizations. However, Namibia continues to have had a problem with the high rate of learner pregnancy and school drop-out for many years. Previous studies reported the absences and limitations of both internal and external actors' collaboration in its implementation. This paper aims to clarify the lack of stakeholder involvement and cooperation in the ESPPMLP activities within selected rural schools in Namibia.MethodsUsing the qualitative approach mixed with phenomenological and content data analysis, the study conducted 19 individual interviews with schoolteachers, one school board member, one NGO representative, and six focus group discussions with 27 parents.Results and findingsAmong others, results show the limited life skills of teachers in schools, the attitude of the community toward schoolteachers, making them feel devoid of influence in collaborations, and the lack of sexual reproductive activities programs in school settings. The findings reveal the reliance of the activities of prevention and management of learner pregnancy on donor-driven initiatives, the lack of task forces at the community level, the shortage of knowledge and understanding about the ESPPMLP and the lack of policy implementation guidelines. Establish learner pregnancy leadership structures at the community level for coordination, introduce community parenting, and execute partnership of monitoring activities to boost stakeholder collaborations.
A glimpse of social presence during an organic chemistry group activity
Authors: Ballard Joy , Winfield Leyte 
Published: April 2024

IntroductionThe US must strengthen the STEM workforce to provide solutions that support a resilient society and robust economy. Such solutions require a range of perspectives from individuals with diverse identities. However, women and individuals who identify as a racial or ethnic minority are woefully underrepresented in the STEM workforce. The reality doubly impacts those with intersecting identities, such as Black women. Efforts to increase the representation of Black women in STEM disciplines include interventions that improve their success in undergraduate courses. Paramount to this is expanding understanding of the experiences of Black women in the academic setting. The current work is situated within the social presence of the Community of Inquiry (CoI) framework, examining social talk among Black women in the learning environment. The CoI model holds that the interconnection between teaching, cognitive, and social presences influences how students engage to master course content. More specifically, each presence is critical for the student’s ability to connect with their peers and instructor and engage in discourse related to the application of information.MethodsThe characterization of social presence was captured through audio and visual recordings. The videos were examined for academic and non-academic social talk. The group examined consisted of four Black women at a HBCU.ResultsInstances of social talk were captured that aligned with affective and emotional expression, open communication, and group cohesion. The dialog was both academic in nature, corresponding to the task, and non-academic, unrelated to the task.DiscussionFew studies have been done to characterize CoI in academic spaces consisting only of Black women. Disaggregating the stories of Black women from the broader context provides the visibility needed to understand their experiences and imagine a STEM paradigm responsive to their existence. Such knowledge is critical to understanding better how women, particularly women of African descent, navigate the learning environment. The article provides a preliminary look at Black women in an active learning setting to shed light on the experiences of these individuals in the STEM context.
Non-targeted metabolomics revealed novel links between serum metabolites and primary ovarian insufficiency: a Mendelian randomization study
Authors: Chen Shuang , Zhou Zhaokai , Zhou Zihan , Liu Yu , Sun Shihao , Huang Kai , Yang Qingling , Guo Yihong 
Published: April 2024

BackgroundPrimary ovarian insufficiency (POI) is a common clinical endocrine disorder with a high heterogeneity in both endocrine hormones and etiological phenotypes. However, the etiology of POI remains unclear. Herein, we unraveled the causality of genetically determined metabolites (GDMs) on POI through Mendelian randomization (MR) study with the overarching goal of disclosing underlying mechanisms.MethodsGenetic links with 486 metabolites were retrieved from GWAS data of 7824 European participants as exposures, while GWAS data concerning POI were utilized as the outcome. Via MR analysis, we selected inverse-variance weighted (IVW) method for primary analysis and several additional MR methods (MR-Egger, weighted median, and MR-PRESSO) for sensitivity analyses. MR-Egger intercept and Cochran’s Q statistical analysis were conducted to assess potential heterogeneity and pleiotropy. In addition, genetic variations in the key target metabolite were scrutinized further. We conducted replication, meta-analysis, and linkage disequilibrium score regression (LDSC) to reinforce our findings. The MR Steiger test and reverse MR analysis were utilized to assess the robustness of genetic directionality. Furthermore, to deeply explore causality, we performed colocalization analysis and metabolic pathway analysis.ResultsVia IVW methods, our study identified 33 metabolites that might exert a causal effect on POI development. X-11437 showed a robustly significant relationship with POI in four MR analysis methods (PIVW=0.0119; Pweighted-median =0.0145; PMR-Egger =0.0499; PMR-PRESSO =0.0248). Among the identified metabolites, N-acetylalanine emerged as the most significant in the primary MR analysis using IVW method, reinforcing its pivotal status as a serum biomarker indicative of an elevated POI risk with the most notable P-value (PIVW=0.0007; PMR-PRESSO =0.0022). Multiple analyses were implemented to further demonstrate the reliability and stability of our deduction of causality. Reverse MR analysis did not provide evidence for the causal effects of POI on 33 metabolites. Colocalization analysis revealed that some causal associations between metabolites and POI might be driven by shared genetic variants.ConclusionBy incorporating genomics with metabolomics, this study sought to offer a comprehensive analysis in causal impact of serum metabolome phenotypes on risks of POI with implications for underlying mechanisms, disease screening and prevention.
Which factors affect the live birth outcome of the first single euploid frozen-thawed blastocyst transfer in couples with balanced chromosomal translocations?
Authors: Zhang Ruixiao , Hu Yahui , Cui Chenchen , Zhang Cuilian 
Published: April 2024

ObjectiveThe objective of this study is to investigate the factors that influence the live birth rate (LBR) of the first single euploid frozen-thawed blastocyst transfer (FBT) cycles after preimplantation genetic testing for structural rearrangements (PGT-SR) in couples with balanced chromosomal translocations (BCT).DesignSingle center, retrospective and observational study.MethodsA total of 336 PGT-SR and the first single euploid FBT cycles between July 2016 and December 2022 were included in this study. The patients were divided into two groups according to the live birth outcomes. The parameters of the study population, controlled ovarian stimulation cycles, and FBT cycles were analyzed. Multivariable binary logistic regression was performed to find the factors that affected the LBR.ResultsThe percentage of blastocysts at developmental stage Day 5 compared to Day 6 (51.8% vs. 30.8%; P&lt;0.001) and with morphology ≥BB compared to &lt;BB (49.7% vs. 32.2%; P=0.001) was significantly different between the group that resulted in live births (n=193) and the group that did not (n=143). The results of the multivariable binary logistic regression analysis indicated that the developmental stage (adjusted OR: 2.068, 95%CI 1.291-3.313; P=0.003) and morphology (adjusted OR: 1.697, 95%CI 1.039-2.773; P=0.035) of the blastocyst were significantly correlated with live birth. Patients with blastocysts that reached the developmental stage at Day 5 and had a morphology of ≥BB had a higher likelihood of having a live birth.ConclusionThe developmental stage and morphology of blastocyst affect the live birth outcome of the first single euploid FBT in BCT carriers undergoing PGT-SR.
Endometrium development patterns and BMI groups among in vitro fertilization patients; prognostic aspects
Authors: Vedelek Viktor , Bicskei Petra , Tábi Mariann , Lajkó Noémi , Ékes Csaba , Bereczki Kristóf , Meixner-Csáti Zsófia , Sinka Rita , Vágvölgyi Anna , Zádori János 
Published: April 2024

IntroductionThe impact of the obesity pandemic on female reproductive capability is a factor that needs to be investigated. In addition, the link between endometrial thickness and in vitro fertilization (IVF) outcomes is contentious.GoalOur goal was to analyze the association among endometrium development, hormone levels, embryo quality, clinical pregnancy, anamnestic parameters, and body mass index (BMI) in women receiving IVF treatment.Patients and methods537 participants undergoing IVF/ICSI cycles with successful oocyte retrieval were enrolled. Subjects were divided into four BMI based groups: underweight (UW; n=32), normal weight (NW; n=324), overweight (OW; n= 115), obesity (OB; n=66). Anthropometric and anamnestic parameters, characteristics of stimulation, endometrial thickness on the day of hCG injection, at puncture, at embryo transfer, FSH, LH, AMH, partner’s age and the semen analysis indicators, embryo quality, clinical pregnancy, were recorded and analyzed. Support Vector Machine (SVM) was built to predict potential pregnancies based on medical data using 22 dimensions.ResultsIn accordance with BMI categories, when examining pregnant/non-pregnant division, the average age of pregnant women was significantly lower in the UW (30.9 ± 4.48 vs. 35.3 ± 5.49 years, p=0.022), NW (34.2 ± 4.25 vs. 36.3 ± 4.84 years, p&lt;0.001), and OW (33.8 ± 4.89 vs. 36.3 ± 5.31 years, p=0.009) groups. Considering FSH, LH, and AMH levels in each BMI category, a statistically significant difference was observed only in the NW category FSH was significantly lower (7.8 ± 2.99 vs. 8.6 ± 3.50 IU/L, p=0.032) and AMH (2.87 ± 2.40 vs. 2.28 ± 2.01 pmol/L, p=0.021) was higher in pregnant women. There were no further statistically significant differences observed between the pregnant and non-pregnant groups across any BMI categories, especially concerning endometrial development. Surprisingly, BMI and weight correlated negatively with FSH (r=-0.252, p&lt;0.001; r=-0.206, p&lt;0.001, respectively) and LH (r= -0.213, p&lt;0.001; r= -0.195, p&lt;0.001) in the whole population. SVM model average accuracy on predictions was 61.71%.DiscussionA convincing correlation between endometrial thickness development and patients’ BMI could not be substantiated. However, FSH and LH levels exhibited a surprising decreasing trend with increasing BMI, supporting the evolutionary selective role of nutritional status. Our SVM model outperforms previous models; however, to confidently predict the outcome of embryo transfer, further optimization is necessary.
Effectiveness of leuprolide acetate administered monthly compared to three-monthly in the treatment of central precocious puberty: evaluation at the end of treatment
Authors: Thaneetrakool Thanaporn , Aroonparkmongkol Suphab , Numsriskulrat Nattakarn , Supornsilchai Vichit , Wacharasindhu Suttipong , Srilanchakon Khomsak 
Published: April 2024

IntroductionGonadotropin-releasing hormone (GnRH) analogs are the standard treatment for central precocious puberty (CPP). Although there are numerous varieties of GnRH agonists, the effectiveness of 1-monthly compared with 3-monthly Leuprolide acetate is still restricted. The objective of this study was to evaluate the outcomes of CPP treatment with Leuprolide acetate at a 1-monthly dosage of 3.75 mg, in comparison to a dosage of 11.25 mg administered every 3 months.MethodThis retrospective cohort study involved 143 girls diagnosed with CPP with 72 of them receiving the monthly treatment regimen and 71 receiving the 3-monthly treatment regimen. Anthropometric measurements were compared at the start and end of the therapy. The rates and level of LH suppression were assessed six months after therapy.ResultsThe regimen administered every 3 months showed more significant suppression of LH. The 3-monthly group showed lower actual height and degree of bone age advancement at the end of therapy. However, the predicted adult height (PAH) remained comparable in both groups.ConclusionThe 3-monthly treatment showed greater hormonal and growth suppression effects, but there was no significant difference in PAH between the two groups.
Evaluating the impact of urban landscape elements on the sense of security and local belonging-case study: Tongdejie, China
Authors: Zhu Yue , Du Ruichao 
Published: April 2024

Introduction: The urban landscape is a pivotal element shaping the urban space and significantly influences its desirability or undesirability. It has a profound connection with people and their living environment, playing a crucial role in providing identity and a sense of peace to citizens. As urbanization expands and population density increases, the importance of security as a basic need within the urban structure becomes even more significant. A confusing, unattractive, or monotonous urban space can adversely affect citizens’ feelings and reduce their sense of security.Methods: In this research, we investigated the relationship between urban landscape components and the sense of local belonging, as well as the relationship between the sense of security and the urban landscape. We sought to understand how the design and planning of the urban landscape can have a positive impact on urban life and increase citizens’ sense of belonging to the city and its surroundings.Results: Our findings revealed a positive and significant correlation between the sense of local belonging and the sense of security. Living in a safe, attractive, and desirable environment fosters and strengthens feelings of belonging to the environment. This strengthening of the sense of belonging increases residents’ satisfaction and enhances the quality of life in cities.Discussion: The urban landscape serves as a powerful tool that, when optimally designed, can positively influence urban life and enhance citizens’ sense of belonging to their city and its surrounding environment. By prioritizing security and creating aesthetically pleasing and engaging urban spaces, cities can significantly improve the well-being and quality of life of their residents.
Use of remote sensing and image processing for identification of wild orchids
Authors: Ahmed Shara , Lightbown Jack , Rutter Simon R. , Basu Nabanita , Nicholson Catherine E. , Perry Justin J. , Dean John R. 
Published: April 2024

A novel multi-technique approach has been applied for the identification and mapping of wild orchids using a combination of remote sensing and spectral image analysis. The five orchid species identified were the common spotted-orchid (Dactylorhiza fuchsia), heath spotted-orchid (Dactylorhiza maculata), pyramidal orchid (Anacamptis pyramidalis), heath fragrant-orchid (Gymnadenia borealis), and the dark-red helleborine (Epipactis atrorubens). Field studies have been done using a hand-held spectrometer operating in the 400–700 nm visible spectrum, photogrammetry using a digital camera as well as a multispectral image camera operating at the specific spectral bands of 450 nm (blue), 560 nm (green), 650 nm (red), 730 nm (red edge) and 840 nm (near-infrared) attached to an unmanned aerial vehicle Data analysis, using the hand-held spectrometer, followed by pattern recognition using principal component analysis and partial least squares-discriminant analysis, have identified the key distinguishing wavelengths for identification of the 5 orchid types as 400, 410, 420 and 560 nm. The use of remote sensing, using the UAV-MSI, and application of a dedicated spectral index has enabled field identification of the orchids. Finally, object-based image analysis of field gathered photogrammetry imagery, has enabled use of shape, size, and color to identify and distinguish orchid species. The developed data analytic tool, using random forest classification, can be used to identify and characterize wild orchids across multiple sites within their short lifespan with an accuracy of 86%. Any longer-term study would provide invaluable information on the diversity and complexity of orchid habitat, population variation both intra- and inter-site location, as well as the impact of climate change.
Genomic prediction based on a joint reference population for the Xinjiang Brown cattle
Authors: Zhang Menghua , Xu Lei , Lu Haibo , Luo Hanpeng , Zhou Jinghang , Wang Dan , Zhang Xiaoxue , Huang Xixia , Wang Yachun 
Published: April 2024

Introduction: Xinjiang Brown cattle constitute the largest breed of cattle in Xinjiang. Therefore, it is crucial to establish a genomic evaluation system, especially for those with low levels of breed improvement.Methods: This study aimed to establish a cross breed joint reference population by analyzing the genetic structure of 485 Xinjiang Brown cattle and 2,633 Chinese Holstein cattle (Illumina GeneSeek GGP bovine 150 K chip). The Bayes method single-step genome-wide best linear unbiased prediction was used to conduct a genomic evaluation of the joint reference population for the milk traits of Xinjiang Brown cattle. The reference population of Chinese Holstein cattle was randomly divided into groups to construct the joint reference population. By comparing the prediction accuracy, estimation bias, and inflation coefficient of the validation population, the optimal number of joint reference populations was determined.Results and Discussion: The results indicated a distinct genetic structure difference between the two breeds of adult cows, and both breeds should be considered when constructing multi-breed joint reference and validation populations. The reliability range of genome prediction of milk traits in the joint reference population was 0.142–0.465. Initially, it was determined that the inclusion of 600 and 900 Chinese Holstein cattle in the joint reference population positively impacted the genomic prediction of Xinjiang Brown cattle to certain extent. It was feasible to incorporate the Chinese Holstein into Xinjiang Brown cattle population to form a joint reference population for multi-breed genomic evaluation. However, for different Xinjiang Brown cattle populations, a fixed number of Chinese Holstein cattle cannot be directly added during multi-breed genomic selection. Pre-evaluation analysis based on the genetic structure, kinship, and other factors of the current population is required to ensure the authenticity and reliability of genomic predictions and improve estimation accuracy.
Transcriptome and metabolome for identifying key metabolites impacting the Vibrio parahaemolyticus in Litopenaeus vannamei
Authors: Jin Jiaojiao , Chen Nanxiong , Hu Yicheng , Chen Yibin , Yin Bin , Wang Xu , Shen Yuchun , Li Sedong 
Published: April 2024

IntroductionShrimp is an important aquaculture species worldwide. Vibrio parahaemolyticus (VP) is an opportunistic pathogen of Litopenaeus vannamei that can cause diseases such as acute hepatopancreatic necrotic disease (AHPND), resulting in significant losses to the shrimp farming industry.MethodsIn this study, We analyzed two shrimp populations by transcriptomics and non-targeted metabolomics, which exhibited significant differences in resistance to VP. Through integrated analysis, we identified genes and metabolites linked to the development of shrimp's resistance to VP.Results and discussionThe analysis revealed that the differential metabolism of flavonoid compounds, especially quercetin, significantly influences the expression of shrimp's resistance to VP. Supplementing feed with an appropriate quantity of quercetin has the potential to increase the expression of crucial genes in the NF-κB pathway, including TLR and AP1, along with the expression of the antibacterial peptide crustin, resulting in a decreased mortality rate. Together, these results indicate that an appropriate amount of quercetin can strengthen the immune response of shrimp to VP, thereby reducing the incidence of AHPND.
Clinical factors associated with subsequent surgical intervention in women undergoing early medical termination of viable or non-viable pregnancies
Authors: Au Heng-Kien , Liu Chi-Feng , Chien Li-Wei 
Published: April 2024

IntroductionMifepristone-misoprostol treatment for medical abortion and miscarriage are safe and effective. This study aimed to assess clinical factors associated with subsequent surgical intervention after medical termination of early viable or non-viable pregnancy.MethodsThis retrospective, single-center study included women who underwent medical abortion at Taipei Medical University between January 2010 and December 2019. A total of 1,561 subjects, with 1,080 viable and 481 non-viable pregnancies, who were treated with oral mifepristone 600 mg followed by misoprostol 600 mg 48 h later were included. Data of all pregnancies and medical termination of pregnancy were evaluated using regression analysis. The main outcome was successful termination of pregnancy.ResultsThe success rate of medical abortion was comparable in women with viable and non-viable (92.13% vs. 92.93%) pregnancies. Besides retained tissue, more existing pregnancies with ultrasonographic findings were found in the non-viable pregnancy group than in the viable pregnancy group (29.4% vs. 14.1%, p = 0.011). Multivariate analysis showed that previous delivery was an independent risk factor for failed medical abortion among all included cases. In women with viable pregnancy, longer gestational age [adjusted odds ratio (aOR): 1.483, 95% confidence interval (CI): 1.224–1.797, p &lt; 0.001] and previous Cesarean delivery (aOR: 2.177, 95% CI: 1.167–40.62, p = 0.014) were independent risk factors for failed medical abortion. Number of Cesarean deliveries (aOR: 1.448, 95% CI: 1.029–2.039, p = 0.034) was an independent risk factor for failed medication abortion in women with non-viable pregnancies.ConclusionThis is the first cohort study to identify risk factors for subsequent surgical intervention in women with viable or non-viable pregnancies who had undergone early medically induced abortions. The success rate of medical abortion is comparable in women with viable and non-viable pregnancies. Previous delivery is an independent risk factor for failed medical abortion. Clinical follow-up may be necessary for women who are at risk of subsequent surgical intervention.