bluuebunny/crossref_metadata_2025_split huggingface dataset:
APPLICATION OF ARTIFICIAL NEURAL NETWORK TO PREDICTION OF CANTONESE SOY SAUCE BREWING AND CHANGING PATTERN CONCERNING TOTAL NITROGEN AND α‐AMINO ACID NITROGEN
Authors: YANG LAN , ZHAO MOU‐MING , ZHAO HAI‐FENG , SU GUO‐WAN , GAO XIAN‐LI 
Published: May 2010

ABSTRACTIn the present study, the artificial neural network was used to predict the brewing of traditional Cantonese soy sauce and its changing pattern with regard to total nitrogen (TN) andα‐amino acidic nitrogen (AN). The effects of parameters during brewing on the contents of TN and AN were modeled by various networks. After the 7‐40‐2 network was determined statistically, it was used to extract the possible correlations between TN and AN, and the relative significance of some factors. It was found that the change of TN and AN contents with the change of time and acidic protease activity was strongly correlated with an R value of 0.9848 and 0.9916, respectively. As for the relative significances of inputs, aging time is the key factor for both outputs during the moromi aging. And for other inputs, it was temperature > pH > neutral protease activity > acid protease activity for TN, and temperature > acidic protease activity > neutral protease activity > pH for AN, respectively.PRACTICAL APPLICATIONSBecause of its open‐air brewing and lengthy brewing time of Cantonese soy sauce, it was difficult to predict its quality indices, the contents of total nitrogen and α‐amino acidic nitrogen, which would change with outside factors (time and temperature) and inside factors (protease activity and pH values). So the study on brewing‐state predication and changing pattern of quality indices were performed by using the artificial neural network on an experimental scale. And results from this preliminary study would help manufacturers to foresee the change and contents of quality indices accurately in the brewing process on an industrial scale. Furthermore, it was anticipated that more effective control and improvement on the quality of traditional Cantonese would be achieved in the future study. Finally, the trained network also can be used in the research of online prediction for other time‐consuming natural fermentation.
Changes in nursing home residents during an innovation based on the theory of gerotranscendence
Authors: Wadensten Barbro 
Published: May 2010

wadensten b. (2010) Changes in nursing home residents during an innovation based on the theory of gerotranscendence. International Journal of Older People Nursing 5, 108–115Aims and objectives.  The aim of the present study was to investigate how residents were affected when an intervention based on the theory of gerotranscendence and guidelines derived from the theory was introduced in a nursing home.Background.  The notion that communication is essential in staff‐resident interaction is widely accepted both in theory and practise, as is the notion that communicative behaviour influences patient outcomes. No studies as yet have focused on whether residents are influenced by staff use of guidelines derived from the theory of gerotranscendence.Design.  The study was explorative in nature and used a qualitative approach.Methods.  Data were collected through interviews with residents and participant observations; qualitative content analysis was performed. Sample size: six residents.Results.  The study shows that many residents reviewed and thought about their lives, and that this aspect changed during the study period. Some residents’ descriptions of their situation revealed that certain changes in social life on the ward had occurred. These changes could be an effect of the intervention.Relevance to clinical practice.  For nurses working in gerontological care, it is a challenge to get to know and provide the best nursing care and individual treatment for each resident; this involves a holistic view helping residents in their ageing process. The use of guidelines derived from the theory of gerotranscendence could help nurses promote positive ageing and constitute one way of treating residents in a more individualized manner.
Developing person‐centred practice: nursing outcomes arising from changes to the care environment in residential settings for older people
Authors: McCormack Brendan , Dewing Jan , Breslin Liz , Coyne‐Nevin Ann , Kennedy Kate , Manning Mary , Peelo‐Kilroe Lorna , Tobin Catherine , Slater Paul 
Published: May 2010

mccormack b., dewing j., breslin l., coyne‐nevin a., kennedy k., manning m., peelo‐kilroe l., tobin c. & slater p. (2010) Developing person‐centred practice: nursing outcomes arising from changes to the care environment in residential settings for older people. International Journal of Older People Nursing 5, 93–107Aim.  To present the nursing outcomes from the evaluation of developments in the care environment in residential settings for older people.Design.  The evaluation data reported here is derived from a larger national programme of work that focused on the development of person‐centred practice in residential services for older people using an emancipatory practice development framework. A multi‐method evaluation framework was utilised. Outcome data were collected at three time points between December 2007 and September 2009. The data reported here were collected using an instrument called the ‘Person‐Centred Nursing Index’.Findings.  Heavy workload was the main cause of stress among nurses. Personal and professional satisfaction with the job was scored highest by the total sample of nurses. Nineteen factors were examined using the Person‐Centred Nursing Index. Statistically significant changes were observed in 12 of these. In addition, there were statistically significant changes in nurses’ perceptions of caring, indicating a shift from a dominant focus on ‘technical’ aspects of care, to one where ‘intimate’ aspects of care were more highly valued.Relevance to clinical practice.  The findings highlight the importance of the development of effective teamwork, workload management, time management and staff relationships in order to create a culture where there is a more democratic and inclusive approach to practice and space for the formation of person‐centred relationships.
Anglo‐Italian Cultural Relations before and during the Long Eighteenth Century
Authors: GASH JOHN 
Published: May 2010

AbstractThis introduction sketches some of the key factors and moments in Anglo‐Italian contact from the Roman occupation of Britain to the emergence of the Grand Tour in the seventeenth century as a channel for cultural interchange. It then indicates some of the changes that occurred in the nature of that interchange during the eighteenth century. These are explored in the ensuing essays, whose subjects range from the impact in Italy of the writings of Hobbes, through the motivations and prejudices of British travellers to the peninsula, to the reciprocal journeys to England of Italian painters and art dealers.
The Changing View of Rome in the Long Eighteenth Century
Authors: SWEET ROSEMARY 
Published: May 2010

AbstractThis article surveys the responses of British travellers to Rome in the long eighteenth century, as expressed in topographical literature, correspondence and diaries, and considers how these were shaped by changing domestic preoccupations. British depictions of the city in its ancient and modern state are compared with the accounts that they would have encountered in the topographical literature and prints available in Rome and in the information offered to them by the local ciceroni. This comparison highlights revealing differences between the Rome that the Romans sought to project and the one the British wished to see.
The Advantages of ‘Demi‐Naturalization’: Mutual Perceptions of Britain and Italy in Hester Lynch Piozzi's Observations and Reflections Made in the Course of a Journey through France, Italy and Germany
Authors: D'EZIO MARIANNA 
Published: May 2010

AbstractIn 1789 Hester Lynch Piozzi's Observations and Reflections Made in ... France, Italy, and Germany appeared in London. The subjectivity featuring in Piozzi's work offers an innovative and romantic version of the Grand Tour, narrated by a woman with an eye to publication. Sensations work as an emotional filter, through a spontaneous flow of personal reflections on Italy, and Piozzi's privileged position as the wife of an Italian – her ‘demi‐naturalization’– gave her an advantage over other British travellers and the freedom to go beyond the classical narrative of Italy and enjoy the pleasure of the unknown.
The Leviathan in Naples: Vico's Response to Hobbes's Life and Works
Authors: SERGIO EMILIO 
Published: May 2010

AbstractDuring Vico's lifetime Hobbes's philosophy was interpreted in various ways: as a product of the new natural philosophy, as an example of political thought inspired by an Epicurean conception of human nature and as a counter to the anti‐royalist party in Naples. Vico's study of Hobbes, probably through his reading of two works in Valletta's library, did not involve an uncritical acceptance of his thought. In Vico's view, Hobbes mistrusted the idea of defining human nature through human history. However, Vico's attitude to Hobbes was not purely negative, and he recognised the importance of Hobbes's thought for modern political debate.
Giambattista Biffi and His Role in the Dissemination of English Culture in Eighteenth‐Century Lombardy
Authors: GUERRA LIA 
Published: May 2010

AbstractThis article explores the relationship between eighteenth‐century English periodicals and the Italian journal Il Caffè, with a particular focus on the role of cultural mediator played by the nobleman Giambattista Biffi, from Cremona. The investigation is based on research into the collection of Biffi's manuscripts (copies of, or translations from, English journals such as The Spectator, The Idler and The Guardian) held at the Biblioteca Statale di Cremona. The article highlights some of Biffi's particular interests and the means by which he was able to infiltrate them into the pages of Il Caffè, even in contributions not written by himself.
Genome‐wide analysis of POU3F2/BRN2 promoter occupancy in human melanoma cells reveals Kitl as a novel regulated target gene
Authors: Kobi Dominique , Steunou Anne‐Lise , Dembélé Doulaye , Legras Stéphanie , Larue Lionel , Nieto Laurence , Davidson Irwin 
Published: May 2010

SummaryPOU3F2 is a POU‐Homeodomain transcription factor expressed in neurons and melanoma cells. In melanoma lesions, cells expressing high levels of POU3F2 show enhanced invasive and metastatic capacity that can in part be explained by repression of Micropthalmia‐associated Transcription Factor (MITF) expression via POU3F2 binding to its promoter. To identify other POU3F2 target genes that may be involved in modulating the properties of melanoma cells, we performed ChIP‐chip experiments in 501Mel melanoma cells. 2108 binding loci located in the regulatory regions of 1700 potential target genes were identified. Bioinformatic and experimental assays showed the presence of known POU3F2‐binding motifs, but also many AT‐rich sequences with only partial similarity to the known motifs at the occupied loci. Functional analysis indicates that POU3F2 regulates the stem cell factor (Kit ligand, Kitl) promoter via a cluster of four closely spaced binding sites located in the proximal promoter. Our results suggest that POU3F2 may regulate the properties of melanoma cells via autocrine KIT ligand signalling.
Outcome of medical treatment for horses with foot pain: 56 cases
Authors: GUTIERREZ‐NIBEYRO S. D. , WHITE II N. A. , WERPY N. M. 
Published: May 2010

SummaryReasons for performing study:Currently, there are limited data regarding the long‐term outcome of horses with foot pain treated with corrective shoeing, rest and rehabilitation, and intrasynovial anti‐inflammatory medication to target lesions detected with MRI.Objective:To report the long‐term (≥12 months) outcome of horses with foot lesions following medical therapy.Hypotheses:1) There is no association between clinical parameters considered and a poor response to therapy. 2) Horses with a deep digital flexor tendinopathy are less likely to respond to medical therapy than horses without a deep digital flexor tendinopathy.Methods:The medical records of horses with foot pain subjected to MRI examination and medical therapy (2005–2007) were evaluated retrospectively. Data collected included history, signalment, occupation, duration and severity of lameness at the time of MRI, radiological and MRI abnormalities. Number of treatments, complications and long‐term response to treatment were obtained by detailed telephone questionnaires. Association between clinical and MRI findings and long‐term lameness were investigated.Results:Frequent abnormal structures included the navicular bone, the deep digital flexor tendon, the navicular bursa and the distal interphalangeal joint. Thirty‐four of 56 horses (60.7%) failed to return to previous level of exercise due to persistent or recurrent lameness or owners' decision to decrease the horse's athletic level; however, 11 horses (32.3%) were being used for light riding. Prognosis for horses with concurrent deep digital flexor tendon, navicular bone and navicular bursa lesions was worse than horses with individual lesions. Deep digital flexor tendinopathy was strongly associated with persistent or recurrent lameness.Conclusions:Horses with multiple foot lesions managed with conservative therapy have a guarded prognosis for long‐term soundness. Deep digital flexor tendinopathies negatively influence prognosis.
Colic in geriatric compared to mature nongeriatric horses. Part 1: Retrospective review of clinical and laboratory data
Authors: SOUTHWOOD L. L. , GASSERT T. , LINDBORG S. 
Published: May 2010

SummaryReason for performing study: It is the impression of some surgeons that geriatric horses have a lower survival rate compared to mature nongeriatric horses following colic surgery. One possible reason for this is that geriatric horses may be more critically ill at admission and have more severe disease than mature nongeriatric horses.Objective: To compare admission historical, physical examination and laboratory data for geriatric and mature nongeriatric horses referred for signs of colic.Methods: Medical records of horses admitted with a presenting complaint of colic between 2000 and 2006 were reviewed. Geriatric horses ≥16 years (n = 300) and mature nongeriatric horses 4–15 years (n = 300). Information obtained included duration of colic prior to admission, admission level of pain, heart rate, intestinal borborygmi, packed cell volume (PCV), plasma creatinine and blood lactate concentrations and peritoneal fluid total protein. Data were analysed using a Chi‐squared test or an analysis of variance. Level of significance was P<0.05.Results: There was no difference between geriatric and mature horses in the duration of colic prior to admission or in admission heart rate, PCV, or plasma creatinine or blood lactate concentrations. However, geriatric horses were more likely to be moderately painful and less likely to be bright and alert than mature horses; and less likely to have normal intestinal borborygmi than mature horses. Peritoneal fluid total protein concentration was higher in geriatric than mature horses.Conclusions and potential relevance: Geriatric horses presenting with signs of colic had a similar admission cardiovascular status based on heart rate, PCV, and plasma creatinine and blood lactate concentration to mature horses. Geriatric horses, however, may have different causes of colic, which may be more serious than mature horses based on pain, lack of intestinal borborygmi and peritoneal fluid total protein concentration.
A DECISION-MAKER'S TOOL FOR SUSTAINABILITY-CENTRED STRATEGIC ENVIRONMENTAL ASSESSMENT
Authors: CROAL PETER , GIBSON ROBERT B. , ALTON CHARLES , BROWNLIE SUSIE , WINDIBANK ERIN 
Published: May 2010

This paper outlines a Decision-Maker's Tool (DM Tool), designed to guide practitioners and their inter-disciplinary teams through a typical strategic environmental assessment (SEA) process. While SEA properly includes post-decision follow-up, the DM Tool covers the SEA process up to the creation of a Briefing Note for the decision maker. Together, use of the DM Tool and the Briefing Note should facilitate positive contributions to sustainability through well considered and aligned policies, plans and programmes (PPPs), by enhancing the comprehensiveness, consistency, clarity, accessibility and credibility of decision making information. The discussion presumes that the SEA is central to the PPP development process, rather than being a separate exercise. The DM Tool and Briefing Note are designed to recommend PPP action based on clearly stated needs and purposes, addressing the key issues, and application of explicit sustainability criteria in the comparative evaluation of feasible alternatives. Particular attention is paid to recognising trade-offs and residual risks, and presenting all this information concisely for the decision maker.
Pathways of Proliferation and Antiapoptosis Driven in Breast Cancer Stem Cells by Stem Cell Protein Piwil2
Authors: Lee Jae Ho , Jung Cornelia , Javadian-Elyaderani Parisa , Schweyer Stefan , Schütte Dorothea , Shoukier Moneef , Karimi-Busheri Feridoun , Weinfeld Michael , Rasouli-Nia Aghdass , Hengstler Jan G. , Mantilla Alejandra , Soleimanpour-Lichaei Hamid Reza , Engel Wolfgang , Robson Craig N. , Nayernia Karim 
Published: May 2010

Abstract
Cancer stem cell studies may improve understanding of tumor pathophysiology and identify more effective strategies for cancer treatment. In a variety of organisms, Piwil2 has been implicated in multiple roles including stem cell self-renewal, RNA silencing, and translational control. In this study, we documented specific expression of the stem cell protein Piwil2 in breast cancer with predominant expression in breast cancer stem cells. In patients who were evaluated, we determined that 90% of invasive carcinomas and 81% of carcinomas in situ exhibited highest expression of Piwil2. In breast cancer cells, Piwil2 silencing suppressed the expression of signal transducer and activator of transcription 3, a pivotal regulator of Bcl-XL and cyclin D1, whose downregulation paralleled a reduction in cell proliferation and survival. Our findings define Piwil2 and its effector signaling pathways as key factors in the proliferation and survival of breast cancer stem cells. Cancer Res; 70(11); 4569–79. ©2010 AACR.
Oncogene-Induced Sensitization to Chemotherapy-Induced Death Requires Induction as well as Deregulation of E2F1
Authors: Helgason G. Vignir , O'Prey Jim , Ryan Kevin M. 
Published: May 2010

Abstract
The analysis of DNA tumor viruses has provided landmark insights into the molecular pathogenesis of cancer. A paradigm for this field has been the study of the adenoviral E1a protein, which has led to the identification of proteins such as p300, p400, and members of the retinoblastoma family. Through binding Rb family members, E1a causes deregulation of E2F proteins—an event common to most human cancers and a central pathway in which oncogenes, including E1a, sensitize cells to chemotherapy-induced programmed cell death. We report here, however, that E1a not only causes deregulation of E2F, but importantly that it also causes the posttranscriptional upregulation of E2F1 protein levels. This effect is distinct from the deregulation of E2F1, however, as mutants of E2F1 impaired in pRb binding are induced by E1a and E2F1 induction can also be observed in Rb-null cells. Analysis of E1a mutants selectively deficient in cellular protein binding revealed that induction of E2F1 is instead intrinsically linked to p400. Mutants unable to bind p400, despite being able to deregulate E2F1, do not increase E2F1 protein levels and they do not sensitize cells to apoptotic death. These mutants can, however, be complemented by either the knockdown of p400, resulting in the restoration of the ability to induce E2F1, or by the overexpression of E2F1, with both events reenabling sensitization to chemotherapy-induced death. Due to the frequent deregulation of E2F1 in human cancer, these studies reveal potentially important insights into E2F1-mediated chemotherapeutic responses that may aid the development of novel targeted therapies for malignant disease. Cancer Res; 70(10); 4074–80. ©2010 AACR.
Inhibition of Histone Deacetylase in Cancer Cells Slows Down Replication Forks, Activates Dormant Origins, and Induces DNA Damage
Authors: Conti Chiara , Leo Elisabetta , Eichler Gabriel S. , Sordet Olivier , Martin Melvenia M. , Fan Angela , Aladjem Mirit I. , Pommier Yves 
Published: May 2010

Abstract
Protein acetylation is a reversible process regulated by histone deacetylases (HDAC) that is often altered in human cancers. Suberoylanilide hydroxamic acid (SAHA) is the first HDAC inhibitor to be approved for clinical use as an anticancer agent. Given that histone acetylation is a key determinant of chromatin structure, we investigated how SAHA may affect DNA replication and integrity to gain deeper insights into the basis for its anticancer activity. Nuclear replication factories were visualized with confocal immunofluorescence microscopy and single-replicon analyses were conducted by genome-wide molecular combing after pulse labeling with two thymidine analogues. We found that pharmacologic concentrations of SAHA induce replication-mediated DNA damage with activation of histone γH2AX. Single DNA molecule analyses indicated slowdown in replication speed along with activation of dormant replication origins in response to SAHA. Similar results were obtained using siRNA-mediated depletion of HDAC3 expression, implicating this HDAC member as a likely target in the SAHA response. Activation of dormant origins was confirmed by molecular analyses of the β-globin locus control region. Our findings demonstrate that SAHA produces profound alterations in DNA replication that cause DNA damage, establishing a critical link between robust chromatin acetylation and DNA replication in human cancer cells. Cancer Res; 70(11); 4470–80. ©2010 AACR.
The Limitations of the G1-S Checkpoint
Authors: Deckbar Dorothee , Stiff Thomas , Koch Barbara , Reis Caroline , Löbrich Markus , Jeggo Penny A. 
Published: May 2010

Abstract
It has been proposed that the G1-S checkpoint is the critical regulator of genomic stability, preventing the cell cycle progression of cells with a single DNA double-strand break. Using fluorescence-activated cell sorting analysis of asynchronous cells and microscopic analysis of asynchronous and synchronized cells, we show that full blockage of S-phase entry is only observed &gt;4 hours after irradiation. The process is ataxia-telangiectasia mutated (ATM) dependent and Chk1/2 independent and can be activated throughout G1 phase. By monitoring S-phase entry of irradiated synchronized cells, we show that the duration of arrest is dose dependent, with S-phase entry recommencing after arrest with kinetics similar to that observed in unirradiated cells. Thus, G1-S checkpoint arrest is not always permanent. Following exposure to higher doses (≥2 Gy), G1-S arrest is inefficiently maintained, allowing progression of G1-phase cells into G2 with elevated γH2AX foci and chromosome breaks. At early times after irradiation (≤4 h), G1-S checkpoint arrest is not established but cells enter S phase at a reduced rate. This early slowing in S-phase entry is ATM and Chk2 dependent and detectable after 100 mGy, showing a novel and sensitive damage response. However, the time needed to establish G1-S checkpoint arrest provides a window when cells can progress to G2 and form chromosome breaks. Our findings detail the efficacy of the G1-S checkpoint and define two significant limitations: At early times after IR, the activated checkpoint fails to efficiently prevent S-phase entry, and at later times, the checkpoint is inefficiently maintained. Cancer Res; 70(11); 4412–21. ©2010 AACR.