bluuebunny/crossref_metadata_2025_split huggingface dataset:
Solvent‐Free Chemoselective Cyanation of α,α‐Dibromoacetophenones Using Potassium Hexacyanoferrate(II) as an Eco‐Friendly Cyanide Source
Authors: Zhao Zhouxing , Li Zheng 
Published: August 2010

AbstractA chemoselective route for the synthesis of 2‐aryl‐3,3‐dibromoacrylonitriles by solvent‐free cyanation of α,α‐dibromoacetophenones by using potassium hexacyanoferrate(II) as an eco‐friendly cyanide source is developed. The merits of this procedure include avoiding the use of a strong toxic cyanide source, a catalyst, and volatile organic solvents and the high yields of the products.
Convenient Synthesis of Tetrahydro‐γ‐carbolines and Tetrahydroquinolines through a Chemo‐ and Regioselectivity Switch by a Brønsted Acid Catalyzed, One‐Pot, Multicomponent Reaction
Authors: Cheng Hong‐Gang , Chen Cai‐Bao , Tan Fen , Chang Ning‐Jie , Chen Jia‐Rong , Xiao Wen‐Jing 
Published: August 2010

AbstractAn efficient, one‐pot, multicomponent reaction of aldehydes 1, p‐methoxyaniline (2a), and 2‐vinylindoles 3 was developed. This approach provides a practical approach to synthetically and biologically significant tetrahydro‐γ‐carboline and tetrahydroquinoline derivatives in good yields through a chemo‐ and regioselectivity switch, which can be tuned by simply changing the substituent on the indole component under identical reaction conditions.
A Cyclic Porphyrin Tetramer Linked by Azo and Butadiyne Bridges (Eur. J. Org. Chem. 23/2010)
Authors: Bašić Bruno , McMurtrie John C. , Arnold Dennis P. 
Published: August 2010

AbstractThe cover picture shows the tetranickel complex of a rectangular porphyrin array with novel connectivity. Conjugating azo and butadiyne bridges link the opposite sides of the rectangle, and the absorption spectrum demonstrates strong electronic communication between the chromophores. The synthesis and characterisation of this tetramer are described in the article by D. P. Arnold et al. on p. 4381 f. The molecule is set against a background of a sunset on the Great Barrier Reef, emphasising the role of porphyrinoid pigments in solar energy capture.
Protein phosphorylation in mitochondria – A study on fermentative and respiratory growth ofSaccharomyces cerevisiae
Authors: Ohlmeier Steffen , Hiltunen J. Kalervo , Bergmann Ulrich 
Published: August 2010

AbstractPhosphorylation as a posttranslational protein modification is a common subject of proteomic studies, but phosphorylation in mitochondria is still poorly investigated. The study presented here applied 2‐DE to characterize phosphorylation in the yeast mitochondrial proteome and identified 59 spots corresponding to 34 phosphorylated mitochondrial or mitochondria‐associated proteins. Most of these proteins presented putative substrates of mitogen‐activated protein and target of rapamycin kinases, cAMP‐dependent protein kinase, cyclin‐dependent kinases and Snf1p suggesting them as key players in the phosphorylation of mitochondrial or mitochondria‐associated proteins. The dynamic behaviour of the phosphoproteome under a major metabolic change, the shift from fermentation to respiration (diauxic shift), was further studied. Eight proteins (Ald4p, Eft1p/2p, Eno1p, Eno2p, Om14p, Pda1p, Qcr2p, Sdh1p) had growth dependent changes in their phosphorylation, indicating a role of phosphorylation‐dependent regulation of translation, metabolic pathways (e.g.glucose fermentation, tricarboxylic acid cycle, pyruvate dehydrogenase and its bypass) and respiratory chain.
Construction of two fluorescence‐labeled non‐combined DNA index system miniSTR multiplex systems to analyze degraded DNA samples in the Chinese Han Population
Authors: Bai Xue , Li Shujin , Cong Bin , Li Xia , Guo Xia , He Lujun , Ye Jian , Pei Li 
Published: August 2010

AbstractMiniSTR loci have been demonstrated to be an effective approach in recovering genetic information from degraded specimens, because of the reduced PCR amplicon sizes which improved the PCR efficiency. Eight non‐combined DNA index system miniSTR loci suitable for the Chinese Han Population were analyzed in 300 unrelated Chinese Han individuals using two novel five fluorescence‐labeled miniSTR multiplex systems(multiplex I: D10S1248, D2S441, D1S1677 and D9S2157; multiplex II: D9S1122, D10S1435, D12ATA63, D2S1776 and Amelogenin). The allele frequency distribution and forensic parameters in the Chinese Han Population were reported in this article. The Exact Test demonstrated that all loci surveyed here were found to be no deviation from Hardy–Weinberg equilibrium. The accumulated power of discrimination and power of exclusion for the eight loci were 0.999999992 and 0.98, respectively. The highly degraded DNA from artificially degraded samples and the degraded forensic case work samples was assessed with the two miniSTR multiplex systems, and the results showed that the systems were quite effective.
Lamp‐based wavelength‐resolved fluorescence detection for protein capillary electrophoresis: Setup and detector performance
Authors: de Kort Bregje J. , de Jong Gerhardus J. , Somsen Govert W. 
Published: August 2010

AbstractA lamp‐based fluorescence detection (Flu) system for CE was extended with a wavelength‐resolved (WR) detector to allow recording of full protein emission spectra. WRFlu was achieved using a fluorescence cell that employs optical fibres to lead excitation light from a Xe‐Hg lamp to the capillary window and protein fluorescence emission to a spectrograph equipped with a CCD. A 280 nm band pass filter etc. together with a 300 nm short pass cut‐off filter was used for excitation. A capillary cartridge was modified to hold the detection cell in a commercial CE instrument enabling WRFlu in routine CE. The performance of the WRFlu detection was evaluated and optimised using lysozyme as model protein. Based on reference spectral data, a signal‐intensity adjustment was introduced to correct for transmission losses in the detector optics that occurred for lower protein emission wavelengths. CE‐WRFlu of lysozyme was performed using BGEs of 50 mM sodium phosphate (pH 6.5 or 3.0) and a charged‐polymer coated capillary. Using the 3‐D data set, signal averaging over time and emission‐wavelength intervals was carried out to improve the S/N of emission spectra and electropherograms. The detection limit for lysozyme was 21 nM, providing sufficient sensitivity to obtain spectral information on protein impurities.
Sample stacking capillary electrophoretic microdevice for highly sensitive mini Y short tandem repeat genotyping
Authors: Chen Yuchao , Choi Jong Young , Choi Seok Jin , Seo Tae Seok 
Published: August 2010

AbstractLab‐on‐a‐chip provides an ideal platform for short tandem repeat (STR) genotyping due to its intrinsic low sample consumption, rapid analysis, and high‐throughput capability. One of the challenges, however, in the forensic human identification on the microdevice is the detection sensitivity derived from the nanoliter volume sample handling. To overcome such a sensitivity issue, here we developed a sample stacking CE microdevice for mini Y STR genotyping. The mini Y STR includes redesigned primer sequences to generate smaller‐sized PCR amplicons to enhance the PCR efficiency and the success rate for a low copy number and degraded DNA. The mini Y STR amplicons occupied in the 5‐ and 10‐mm stacking microchannels are preconcentrated efficiently in a defined narrow region through the optimized sample stacking CE scheme, resulting in more than tenfold improved fluorescence peak intensities compared with that of a conventional cross‐injection microcapillary electrophoresis method. Such signal enhancement allows us to successfully analyze the Y STR typing with only 25 pg of male genomic DNA, with high background of female genomic DNA, and with highly degraded male genomic DNA. The combination of the mini Y STR system with the novel sample stacking CE microdevice provides the highly sensitive Y STR typing on a chip, making it promising to perform high‐performance on‐site forensic human identification.
Covariance precoding schemes for MIMO OFDM over transmit‐antenna and path‐correlated channels
Authors: Fu Yu , Krzymień Witold A. , Tellambura Chintha 
Published: August 2010

AbstractThis paper considers covariance‐feedback based linear precoding (LP) and nonlinear Tomlinson–Harashima precoding (THP) for multiple‐input multiple‐output (MIMO) orthogonal frequency‐division multiplexing (OFDM) systems. Orthogonal space–time block coded (OSTBC)‡OFDM and spatially‐multiplexed (SM)§OFDM are analysed. The main objective is to design precoders to mitigate the impact of transmit‐antenna and path correlations. The impact of path correlations on the pairwise error probability (PEP) of MIMO OFDM is also analysed. Closed‐form, waterfilling‐based covariance precoders are derived to minimize the worst case PEP in OSTBC OFDM. An adaptive transmission strategy is also developed for switching between precoded SM OFDM and precoded OSTBC OFDM. The switching criterion is the minimum Euclidean distance of the received codebook. The switching decision sent to the transmitter requires one feedback bit per subcarrier. The proposed precoders considerably reduce the error rate in antenna and path‐correlated channels; nonlinear precoders perform better than linear precoders. We show that the adaptive strategy can achieve full diversity gain, and it outperforms either SM or OSTBC applied individually in terms of the bit error rate (BER). Copyright © 2010 John Wiley & Sons, Ltd.
Inhibitory stimulation of the ventral premotor cortex temporarily interferes with musical beat rate preference
Authors: Kornysheva Katja , von Anshelm‐Schiffer Anne‐Marike , Schubotz Ricarda I. 
Published: August 2010

AbstractBehavioral studies suggest that preference for a beat rate (tempo) in auditory sequences is tightly linked to the motor system. However, from a neuroscientific perspective the contribution of motor‐related brain regions to tempo preference in the auditory domain remains unclear. A recent fMRI study (Kornysheva et al. [2010]: Hum Brain Mapp 31:48‐64) revealed that the activity increase in the left ventral premotor cortex (PMv) is associated with the preference for a tempo of a musical rhythm. The activity increase correlated with how strongly the subjects preferred a tempo. Despite this evidence, it remains uncertain whether an interference with activity in the left PMv affects tempo preference strength. Consequently, we conducted an offline repetitive transcranial magnetic stimulation (rTMS) study, in which the cortical excitability in the left PMv was temporarily reduced. As hypothesized, 0.9 Hz rTMS over the left PMv temporarily affected individual tempo preference strength depending on the individual strength of tempo preference in the control session. Moreover, PMv stimulation temporarily interfered with the stability of individual tempo preference strength within and across sessions. These effects were specific to the preference for tempo in contrast to the preference for timbre, bound to the first half of the experiment following PMv stimulation and could not be explained by an impairment of tempo recognition. Our results corroborate preceding fMRI findings and suggest that activity in the left PMv is part of a network that affects the strength of beat rate preference. Hum Brain Mapp, 2011. © 2010 Wiley‐Liss, Inc.
Effective connectivity of the multiplication network: A functional MRI and multivariate granger causality mapping study
Authors: Krueger Frank , Landgraf Steffen , van der Meer Elke , Deshpande Gopikrishna , Hu Xiaoping 
Published: August 2010

AbstractDevelopmental neuropsychology and functional neuroimaging evidence indicates that simple and complex mental calculation is subserved by a fronto‐parietal network. However, the effective connectivity (connection direction and strength) among regions within the fronto‐parietal network is still unexplored. Combining event‐related fMRI and multivariate Granger Causality Mapping (GCM), we administered a multiplication verification task to healthy participants asking them to solve single and double‐digit multiplications. The goals of our study were first, to identify the effective connectivity of the multiplication network, and second, to compare the effective connectivity patterns between a low and a high arithmetical competence (AC) group. The manipulation of multiplication difficulty revealed a fronto‐parietal network encompassing bilateral intraparietal sulcus (IPS), left pre‐supplementary motor area (PreSMA), left precentral gyrus (PreCG), and right dorsolateral prefrontal cortex (DLPFC). The network was driven by an intraparietal IPS‐IPS circuit hosting a representation of numerical quantity intertwined with a fronto‐parietal DLPFC‐IPS circuit engaged in temporary storage and updating of arithmetic operations. Both circuits received additional inputs from the PreCG and PreSMA playing more of a supportive role in mental calculation. The high AC group compared to the low AC group displayed a greater activation in the right IPS and based its calculation more on a feedback driven intraparietal IPS‐IPS circuit, whereas the low competence group more on a feedback driven fronto‐parietal DLPFC‐IPS circuit. This study provides first evidence that multivariate GCM is a sensitive approach to investigate effective connectivity of mental processes involved in mental calculation and to compare group level performances for different populations. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Framework and methodology for evaluating mental health policy and plans
Authors: Funk Michelle , Freeman Melvyn 
Published: August 2010

AbstractThe importance of monitoring and evaluation for mental health service planning and delivery is indubitable. Notwithstanding, monitoring and evaluation of mental health policy and plans has received only limited attention. This paper presents an approach developed by the World Health Organization for monitoring mental health policy and plans that can be adapted and utilized for evaluation and monitoring of policy and plans in most other health spheres as well. Four critical areas are outlined i.e., evaluation of the policy document and the plan derived from it; monitoring the implementation of the strategic plan; evaluation of the implementation of the plan; and evaluation of whether the objectives of the policy have been achieved. Overcoming difficulties in objective assessment of policy documents and plans is discussed and two WHO checklists for evaluating the process, content and operational aspects of policies and plans are introduced together with a five step guidance process for conducting policy and plan evaluations. Copyright © 2010 John Wiley & Sons, Ltd.
Retracted: Norepinephrine induces VEGF expression and angiogenesis by a hypoxia‐inducible factor‐1α protein‐dependent mechanism
Authors: Park Soon Young , Kang Joo Hee , Jeong Kang Jin , Lee Jangsoon , Han Jeong Whan , Choi Wahn Soo , Kim Yong Kee , Kang Jaeku , Park Chang Gyo , Lee Hoi Young 
Published: August 2010

AbstractA growing number of studies have demonstrated that physiological factors can influence the progression of several cancers via cellular immune function, angiogenesis and metastasis. Recently, stress‐induced catecholamines have been shown to increase the expression of various cancer progressive factors, including vascular endothelial growth factor (VEGF), matrix metalloproteinases and interleukins. However, a detailed mechanism remains to be identified. In this study, we investigated the role of adrenergic receptors and hypoxia‐inducible factor (HIF)‐1α protein in catecholamine‐induced VEGF expression and angiogenesis. Treatment of the cells with norepinephrine (NE) or isoproterenol induced VEGF expression and HIF‐1α protein amount in a dose‐dependent manner. Induction of VEGF expression by NE was abrogated when the cells were transfected with HIF‐1α–specific siRNA. Similarly, adenylate cyclase activator forskolin and cyclic AMP‐dependent protein kinase A inhibitor H‐89 enhanced and decreased HIF‐1α protein amount, respectively. More importantly, conditioned medium of NE‐stimulated cancer cells induced angiogenesis in a HIF‐1α protein–dependent manner. In addition, pretreatment of cells with propranolol, a β‐adrenergic receptor (AR) blocker, completely abolished induction of VEGF expression and HIF‐1α protein amount by NE in all of the tested cancer cells. However, treatment with the α1‐AR blocker prazosin inhibited NE‐induced HIF‐1α protein amount and angiogenesis in SK‐Hep1 and PC‐3 but not MDA‐MB‐231 cells. Collectively, our results suggest that ARs and HIF‐1α protein have critical roles in NE‐induced VEGF expression in cancer cells, leading to stimulation of angiogenesis. These findings will help to understand the mechanism of cancer progression by stress‐induced catecholamines and design therapeutic strategies for cancer angiogenesis.
Decreased expression of updated NESG1 in nasopharyngeal carcinoma: Its potential role and preliminarily functional mechanism
Authors: Liu Zhen , Li Xin , He Xiufang , Jiang Qingping , Xie Siming , Yu Xiaoli , Zhen Yan , Xiao Guanghui , Yao Kaitai , Fang Weiyi 
Published: August 2010

AbstractHuman NESG1 (CCDC19) gene was originally isolated in our laboratory from human nasopharynx tissue. However, the biological and clinical significances of this gene remain largely unknown. In this report, two errors in the originally submitted sequence of human NESG1 gene were found, and the open reading frame sequence of NESG1 (Accession number: NM_012337.1) was revised and updated in the NCBI database (Accession number: NM_012337.2). The antibody raised against the revised sequence of NESG1 detected a single band of 66 kD in human nasopharynx tissues. NESG1 transcripts were specifically expressed in the nasopharynx epithelium. Expression of NESG1 transcripts and protein was downregulated or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines in comparison to that in the normal nasopharynx tissues. The levels of NESG1 protein were significantly greater in the low‐grade NPC tissues than that in the high‐grade NPC tissues. Induced expression of NESG1 in otherwise NESG1‐negative 5‐8F cells not only significantly decreased cell proliferation, G1‐S phase transition, but also markedly inhibited the ability of cell migration and invasion as well as in vivo tumorigenesis. Furthermore, NESG1 also significantly regulated the expression of cell cycle regulator CCNA1 and p21. Our findings first provided evidence that NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.
Predictors and survival of synchronous peritoneal carcinomatosis of colorectal origin: A population‐based study
Authors: Lemmens Valery E. , Klaver Yvonne L. , Verwaal Vic J. , Rutten Harm J. , Coebergh Jan Willem W. , de Hingh Ignace H. 
Published: August 2010

AbstractThe aim of our study was to provide population‐based data on incidence and prognosis of synchronous peritoneal carcinomatosis and to evaluate predictors for its development. Diagnosed in 1995–2008, 18,738 cases of primary colorectal cancer were included. Predictors of peritoneal carcinomatosis were analysed by multivariable logistic regression analysis. Median survival in months was calculated by site of metastasis. In the study period, 904 patients were diagnosed with synchronous peritoneal carcinomatosis (4.8% of total, constituting 24% of patients presenting with M1 disease). The risk of peritoneal carcinomatosis was increased in case of advanced T stage [T4 vs. T1,2: odds ratio (OR) 4.7, confidence limits 4.0–5.6), advanced N stage [N0 vs. N1,2: OR 0.2 (0.1–0.2)], poor differentiation grade [OR 2.1 (1.8–2.5)], younger age [<60 years vs. 70–79 years: OR 1.4 (1.1–1.7)], mucinous adenocarcinoma [OR 2.0 (1.6–2.4)] and right‐sided localisation of primary tumour [left vs. right: OR 0.6 (0.5–0.7)]. Median survival of patients with peritoneum as single site of metastasis remained dismal [1995–2001: 7 (6–9) months; 2002–2008: 8 (6–11) months], contrasting the improvement among patients with liver metastases [1995–2001: 8 (7–9) months; 2002–2008: 12 (11–14) months]. To conclude, synchronous peritoneal metastases from colorectal cancer are more frequent among younger patients and among patients with advanced T stage, mucinous adenocarcinoma, right‐sided tumours and tumours that are poorly differentiated. The prognosis of synchronous peritoneal carcinomatosis remains poor with a median survival of 8 months and even worse if concomitant metastases in other organs are present.
Dietary acrylamide intake and risk of esophageal cancer in a population‐based case‐control study in Sweden
Authors: Lin Yulan , Lagergren Jesper , Lu Yunxia 
Published: August 2010

AbstractAcrylamide is a potential carcinogen, which commonly occurs in some food items. The relation between acrylamide and esophageal cancer deserves attention. In a Swedish nationwide, population‐based case‐control study, data were collected on diet among other variables in 1995–1997 through personal interviews. Included were 189 cases of esophageal adenocarcinoma (participation rate 88%), 262 cases of gastroesophageal junctional adenocarcinoma (84%), 167 cases of esophageal squamous cell carcinoma (73%), and 820 control participants (73%). Dietary intake of acrylamide exposure was assessed from a food‐frequency questionnaire and categorized into quartiles based on the consumption among the control participants. Unconditional logistic regression was used to calculate odds ratios (OR) with 95% confidence intervals (CI), with multivariable adjustment for known risk factors. Among participants in the highest quartile of acrylamide exposure compared to the lowest, the adjusted risk of all esophageal tumors combined was increased (OR 1.23; 95% CI 1.02–1.75), particularly among overweight or obese persons (OR 1.88; 95% CI 1.06–3.34). Increased point risk estimates were found for each type of esophageal cancer, but the association with esophageal squamous cell carcinoma seemed stronger and was statistically significant, particularly among nonsmokers in the highest quartile of acrylamide exposure (adjusted OR 2.82; 95% CI 1.16–6.87). Regarding squamous cell carcinoma only, a dose‐response association was identified (p for trend 0.01). Dietary intake of acrylamide might be a risk exposure for esophageal cancer, a stronger association among overweight or obese persons was indicated.