bluuebunny/crossref_metadata_2025_split huggingface dataset:
Milk-derived haem scavenging microsponges protect heart against ferroptosis-induced reperfusion injury
Authors: Zhu Yang , Zhang Liwen , Wen Jun , Zhang Jiawei , Sun Ziyang , Shen Liyin , Jiang Xiaokai , Dai Binyao , Wang Wenyao , Deng Xiangning , Gao Yun , Jin Qiao , Wang Wenzhao , Wang Min , Xu Feng , Feng Feng , Hua Yuyan , Zhang Jinyue , Gao Changyou , Ren Tanchen , Tang Yida , Guo Chengchen , Fang Xuexian 
Published: June 2024

Abstract

Myocardial ischemia/reperfusion injury with a high incidence of intramyocardial haemorrhage (IMH) contributes to enlarged infarct size by inducing additional cell death and predisposes to risk of heart failure. However, the risk factor in blood remains unverified and unaddressed. Here, we report that haem burstly released from IMH, is the key iron source for ferroptosis, and correspondingly propose the treatment strategy of blocking the cellular uptake of exogenous haem. Unfortunately, there is no existing haem-scavenging materials. We discover that methacryloyl modification of lysine residues on apo-lactoferrin (Apo-Lf), a milk-derived protein screen from natural haem-binding candidates, surprisingly increased the number of haem-binding sites by 86% and binding affinity by one order of magnitude. In animal models, intramyocardially implanted ferroptosis-inhibiting lactoferrin microsponges (FILMS) fabricated from the modified Apo-Lf achieved desirable anti-ferroptosis effects by rapid haem scavenging. Transcatheter FILMS implantation in pigs further demonstrated its safety and translational potential. These results provide deeper mechanistic understanding of ferroptosis-induced I/R injury, and may aid the development of other biomaterial-based therapies.
Genome sequencing of Bacillus sp. isolate EB-40: a plant growth-promoting bacteria
Authors: Pereira Francine Gomes Silva , Vidigal Pedro Marcus Pereira , Santos Samuel A. , Nietsche Silvia , Xavier Adelica Aparecida , Pereira Marlon Cristian Toledo 
Published: June 2024

Abstract

The Bacillussp. isolate EB-40 was characterized in 'Prata Anã' banana (Musasp.) plants as an endophyte capable of colonizing both inter- and intracellular spaces of roots, nitrogen fixation, phosphate solubilization, in vitro synthesis of indole-3-acetic acid, and promotion of enhancements in the development of micropropagated banana seedlings. Here, we report the whole-genome sequence of Bacillus sp. isolate EB-40 and its taxonomic assignment. Its genome is composed of one chromosome and three plasmids. The chromosome is a circular double-stranded DNA (5,613,235 base pairs (bp) ) with a GC content of 35.3% and 5,462 genes. The three plasmids have a total length of 237,685 bp with 201 genes. Comparative genomics highlighted significant conservation of the isolate EB-40 genome with other B. cereus isolates, leading to its assignment it as a novel isolate within this species.
Quality of Life in High-Risk Myeloma Patients Treated with Allogeneic Hematopoietic Cell Transplantation Followed by Bortezomib Maintenance
Authors: Fournier Valentyn , Ogez David , Roy Jean , Leblanc Richard , Ahmad Imran , Lamore Kristopher 
Published: June 2024

Abstract

Purpose. Allogeneic hematopoietic cell transplantation (HCT) is the only curative treatment in multiple myeloma (MM) but is associated with toxicities that impact quality of life (QoL). This study aimed (1) to describe the levels and evolution of QoL scores of newly diagnosed MM patients who received upfront tandem autologous + nonmyeloablative allogeneic HCT, and (2) to evaluate the impact of chronic graft-versus-host disease (cGVHD) on QoL.
Methods. After induction and autologous HCT, patients were invited to participate in a prospective phase II study of tandem nonmyeloablative allogeneic HCT followed by bortezomib maintenance for one year. Participants completed questionnaires assessing QoL and cGVHD before allogeneic HCT (T1), then every three months during treatment (T2 to T6) and after treatment cessation (T7 to T10).
Results. Thirty-three patients were included. Participants had high levels of QoL at all measurement times. Cognitive functioning and global health status decreased significantly during treatment (T1 vs. T2-T5), while fatigue symptoms were reported more frequently. After treatment cessation (T7-T10), only cognitive functioning remained significantly impacted. In contrast, participants reported a better emotional well-being after transplant (T1 vs. T2, T4-T10). Furthermore, as QoL scores were more frequently associated to lung, energy and psychological cGVHD domains.
Conclusion. Our study demonstrates preservation of QoL during this upfront tandem treatment including autologous transplant followed by allogeneic HCT. Some identified domains impacting QoL may support therapeutic actions such as supportive care including psychological and neuropsychological interventions, as well as adapted physical activity in this population.
This trial was registered on 01/12/2014 to ClinicalTrial.gov: NCT02308280.
Investigating the mechanism of rotator cuff tendinopathy induced by type 2 diabetes mellitus in rats using untargeted metabolomics analysis
Authors: Xu kuishuai , Zhang Liang , Wang Tianrui , Yu Tengbo , Zhao Xia , Yu Ning , Zhang Yingze 
Published: June 2024

Abstract

Objective
To assess the mechanism of rotator cuff tendinopathy induced by type 2 diabetes mellitus (T2DM) in rats using untargeted metabolomics analysis.
Methods
The liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics approach was used to screen tendon biomarkers of rotator cuff tendinopathy in rats with T2DM. Seventy-eight Sprague-Dawley rats were divided into normal group (NG) and T2DM groups. Rats in T2DM groups were divided into 12-week (T2DM-12w), and 24-week (T2DM-24w) subgroups according to the time point of the establishment of the T2DM rat model. Histological evaluation (modified Bonar score) and biomechanical testing were used to analyze the adverse effects of type 2 diabetes on the tendon of the supraspinatus muscle in rats.Three comparable groups were set up, including T2DM-12w group vs. NG, T2DM-24w group vs. NG, and T2DM-24w group vs. T2DM-12w group. Differentially expressed metabolites (DEMs) in the supraspinatus tendons in the three groups of rats were analyzed using LC-MS, and data were analyzed using multivariate statistical methods to screen potential biomarkers. The DEMs included in the intersection of the three groups were identified as those associated with the development of diabetic rotator cuff tendinopathy, and trend analysis and pathway topology analysis were performed.
Results
With the progression of diabetes, the tendinopathy of the supracinatus muscle of diabetic rats gradually intensified, mainly manifested as inflammatory reactions, disordered collagen fibers, fat infiltration, and increased modified Bonar score. The intersection of DEMs among the three comparable groups was resulted in the identification of 10 key DEMs, in which melezitose and raffinose showed a continuous increasing trend with the prolongation of disease course. By pathway topology analysis, 10 DEMs (P < 0.01) were mainly associated with the pathways of galactose metabolism, which could be involved in the development of diabetes-induced rotator cuff tendinopathy.
Conclusion
T2DM causes tendinopathy of the supraspinatus muscle in rats. 10 key DEMs obtained by untargeted metabolomics assay suggested that the development of diabetes-induced rotator cuff tendinopathy was associated with changes in metabolic pathways, such as galactose metabolism. melezitose and raffinose hold promise as a biomarker for disease discrimination and/or disease indication in diabetic rotator cuff tendinopathy.
Prediction of Adolescents' Fluid Intelligence Scores based on Deep Learning with Reconstruction Regularization
Authors: Cao TingQian , Liu Xiang , Luo Jiawei , Wang Yuqiang , Huang Shixin 
Published: June 2024

Abstract

Objective
The aim of this study was to develop a predictive model for uncorrected/actual fluid intelligence scores in 9–10 year old children using magnetic resonance T1-weighted imaging. Explore the predictive performance of an autoencoder model based on reconstruction regularization for fluid intelligence in adolescents.
Methods
We collected actual fluid intelligence scores and T1-weighted MRIs of 11,534 adolescents who completed baseline tasks from ABCD Data Release 3.0. A total of 148 ROIs were selected and 604 features were proposed by FreeSurfer segmentation. The training and testing sets were divided in a ratio of 7:3. To predict fluid intelligence scores, we used AE, MLP and classic machine learning models, and compared their performance on the test set. In addition, we explored their performance across gender subpopulations. Moreover, we evaluated the importance of features using the SHapley Additive Explain method. Results: The proposed model achieves optimal performance on the test set for predicting actual fluid intelligence scores (PCC = 0.209 ± 0.02, MSE = 105.212 ± 2.53). Results show that autoencoders with refactoring regularization are significantly more effective than MLPs and classical machine learning models. In addition, all models performed better on female adolescents than on male adolescents. Further analysis of relevant characteristics in different populations revealed that this may be related to gender differences in underlying fluid intelligence mechanisms.
Conclusions
We construct a weak but stable correlation between brain structural features and raw fluid intelligence using autoencoders. Future research may need to explore ensemble regression strategies utilizing multiple machine learning algorithms on multimodal data in order to improve the predictive performance of fluid intelligence based on neuroimaging features.
Preparatory Process in Advance of Runaway Fault Rupture through Fluid Injection Observed in Laboratory Experiments Using a Large Specimen of Sub-meter scale
Authors: Ito Takatoshi , Aoki Koji , Mukuhira Yusuke , Yabe Yasuo 
Published: June 2024

Abstract

Fault slip is initiated by locally applied fluid pressure, and it can expand unstably over a wide area causing elastic waves having magnitudes that induce felt or destructive earthquakes. Thus, it is important to examine the unstable expansion of initial slips. However, it is hard to reproduce the process by general setup of laboratory experiment such as triaxial loading tests on cylindrical specimens with inclined faults. In this study, we prepared a cubic specimen of sub-meter scale, which was separated into two triangular prisms by a model fault. The specimen was subjected to bi-axial compressions with different magnitudes. A 2D array of strain gauges was embedded beneath the fault plane to measure the changes in shear strain with the fault slip driven by fluid injection. Based on the experimental results, we discussed the features of fault slips that lead to injection-induced earthquake. The strain accumulated around the edge of the fault slipping area. The accumulation increased locally the strain by ~ 10 µε, which was equivalent to ~ 0.1 MPa in shear stress. The fault slipping area expanded gradually first, and it expanded unstably beyond the fluid invasion area ~ 3 s later after the slip initiated. The unstable expansion of initial slips was suppressed due to reducing the initial shear stress on the fault by 0.3 MPa. In this case, the initial shear stress was too small for the additional stress accumulated at the edge of the fault slipping area to overcome the static friction on the fault.
The correlation between multi-mode ultrasonographic features of breast cancer and axillary lymph node metastasis
Authors: Hong Zhe , Xu Shijian , Wang Qi 
Published: June 2024

Abstract

Objective
This study aimed to explore the correlation between multi-mode ultrasonographic features of breast cancer and axillary lymph node metastasis.
Method
A total of 196 patients with surgically confirmed breast cancer between September 2019 and December 2023 were included. Preoperative B-mode ultrasound (US), color Doppler, and shear wave elastography (SWE) features of breast cancer masses were collected and analyzed for their correlation with axillary lymph node metastasis. The area under the receiver operating characteristic curve (AUC) of B-mode US, color Doppler, SWE, and the multi-mode predictive model for evaluating axillary lymph node metastasis were compared.
Results
Among the 196 patients, 70 had positive axillary lymph nodes while 126 had negative axillary lymph nodes. There was no significant difference in color features between the negative and positive axillary lymph node groups. Multifocality/multicentricity, architectural distortion, microcalcifications, and the “stiff rim” sign in SWE were identified as independent risk factors for predicting axillary lymph node metastasis according to binary logistic regression analysis. The AUC of the predictive model based on these independent risk factors was 0.803 (95% CI: 0.739–0.867), significantly higher than that of B-mode US or SWE alone.
Conclusion
Multifocality/multicentricity, architectural distortion, microcalcifications, and the “stiff rim” sign in SWE were found to be valuable for predicting axillary lymph node metastasis in patients with breast cancer. The predictive model developed in this study, combining multi-mode ultrasonographic features of breast cancer masses, could serve as a noninvasive and convenient method for predicting axillary lymph node status. This approach could aid in clinical decision-making and individualized treatment to improve the prognosis of breast cancer patients.
Abdominal Epilepsy: Report of two cases and literature review
Authors: Zhang Ying , An Ying , Qi Jialong , Song Zhengji 
Published: June 2024

Abstract

Abdominal epilepsy (AE) is a rare form of epilepsy characterized by paroxysmal abdominal pain accompanied by syncope. It often presents diagnostic challenges due to its diverse clinical manifestations. Herein, we present two cases, a 28-year-old male and a 55-year-old female, with a decade-long history of intermittent abdominal pain and syncope. Despite multiple consultations, a definitive diagnosis remained elusive until electroencephalography (EEG) revealed characteristic abnormalities. Two cases underscore the importance of recognizing AE as a potential cause of unexplained abdominal pain and syncope. Therefore, we recommend that for patients with unexplained abdominal pain accompanied by altered consciousness, after ruling out common causes, consideration should be given to AE. In the meantime, we summarize the possible molecular mechanisms for AE development.
Infant use of acid-suppressant medication, C. difficile colonization, and childhood body mass index and asthma in a Canadian cohort
Authors: Kozyrskyj Anita , Bridgman Sarah , Drall Kelsea , Lau Amanda , Tun Hein , Morales-Lizcano Nadia , Huynh Hein , Mandhane Piush , Moraes Theo , Subbarao Padmaja , Scott James , Simons Elinor , Turvey Stuart 
Published: June 2024

Abstract

Aims Acid-suppressive medication (ASM) therapy during infancy raises risk for C. difficile infection and potentially chronic disease development. We aimed to determine relationships between ASMs given to infants, the proton pump inhibitors (PPI) and histamine-2 receptor antagonists (H2RA), and preschool body mass index (BMI) and asthma and whether they were mediated by C. difficile colonization.Methods ASMs were parent-reported at age 3, 6 and 12 months in 1,025 infants from the CHILD Cohort Study. 16S microbiota sequencing and targeted qPCR for C. difficile was conducted in stool collected at 3-months. At age 5 years, asthma was physician diagnosed and age/sex adjusted BMI-z scores calculated. ASM use, asthma status and BMI-z score associations were assessed by regression, and mediation analyses conducted.Results Approximately 4% of infants received H2RAs, most before age 3 months. C. difficile colonization was twice as likely in 3-month-old infants receiving H2RAs (OR: 2.27, 95% CI: 1.13–4.51) and was associated with higher BMI-z scores at age 5 years (β: 0.31, 95% CI: 0.16, 0.46). Mediation models showed an indirect positive effect of H2RA use on 5-year BMI-z scores, mediated through C. difficile colonization (β: 0.065, 95% CI: 0.01, 0.13). These results were not obtained with PPIs, but sample size was small (1% of infants).Conclusion We found evidence of increased preschool BMI following H2RA use in infants that was mediated through C. difficile colonization. Further study on ASM use in infancy, the gut microbiome and pathways to body weight health outcomes is warranted.
A malignant epithelioid tumor with EWSR1 fusion mimics primary pulmonary myxoid sarcoma (PPMS): a variant of PPMS or under-recognized entity?
Authors: Zhang Bingxia , Li Yuan , Huang Ziling , Jin Yan , Wang Yue , Wang Keke , Li Huan 
Published: June 2024

Abstract

Background
Primary pulmonary myxoid sarcoma (PPMS) is an extremely rare soft tissue tumor that is characterized in most cases by the oncogenic fusion gene EWSR1-CREB1. Tumor cells with epithelioid differentiation have not been described in PPMS. We report a primary lung tumor with morphological, immunohistochemical and molecular features closely mimics PPMS, but with concomitant epithelioid differentiation. Is this a variant of PPMS or under-recognized entity?
Case presentation:
A 34-year-old female patient showed a CT scan showing nodular opacities around the upper lobe of the left lung with a diameter of 8 mm, which was mildly unevenly enhanced after enhancement. Histologically there were two tumor areas, one of which presented a typical PPMS morphology, and the tumor cells were stellate, polygonal, epithelial and arranged as reticular and cords in abundant myxoid stroma. The other tumor area presented an epithelioid cell morphology. The tumor cell immunophenotype was positive for vimentin, INI1 and negative for TTF-1, P40, S-100, SOX10, CK5/6, SMA, Desmin, PAX8, Brachyury and WT1. Interestingly, immunohistochemistry confirmed positive AE1/AE3 in epithelioid cell regions, while AE1/AE3 was negative in typical PPMS regions. Molecular analysis demonstrated the presence of EWSR1-CREB1 gene fusions in the tumor.
Conclusion
PPMS is a very rare lung malignant soft tissue tumor with distinct histological and genetic features. Our challenge case had morphological, immunohistochemical and molecular features similar to PPMS, but with epithelioid differentiation.We prefer to describe this tumour as a variant of PPMS instead of a new entity.
Short- and long-term outcomes after laparoscopic versus open total gastrectomy for stage 0-I gastric cancer: A multicenter, retrospective analysis
Authors: Feng Xingyu , Ye Zaisheng , Zhou Fangyao , Zheng Chengbin , Zhang Zhaojun , Xiong Wenjun , Zeng Yi , Wang Junjiang , Hu Weixian , Zheng Jiabin , Luo Bin , Wang Wei , Chen Luchuan , Li Yong 
Published: June 2024

Abstract

Background
The efficacy and safety of laparoscopic total gastrectomy for early gastric cancer need to be further investigated.
Methods
A retrospective analysis was conducted on the clinicopathological data of 122 patients with stage 0-I gastric cancer underwent radical laparoscopic total gastrectomy (LTG) or open total gastrectomy (OTG) from January 2010 to December 2013. Multivariate regression with a generalized estimation equation (GEE) was used to analyze the differences in total complications, LTG-related complications and OTG-related complications between the laparoscopic and open groups. The inverse probability of treatment weighting (IPTW) Kaplan-Meier survival curve was used to compare the long-term survival of the two groups. The primary outcome was the short-term outcomes and long-term survival of LTG with traditional OTG for stage 0-I gastric cancer.
Results
The incidence of postoperative complications was 16.4% in the LTG group and 18.4% in the OTG group, with no statistically significant difference between the two groups (P > 0.05). In the adjusted multivariate GEE regression for OTG-related complications, the risk of OTG-related complications in the laparoscopic group was 0.111 (95% CI: 0.016–0.771, P = 0.026). Before and after IPTW adjustment, there was no statistically significant difference in survival between the LTG group and the OTG group (P = 0.28 and P = 0.34).
Conclusions
LTG is safe and feasible to apply in stage 0-I gastric cancer. Comparing OTG, LTG with a similar overall complication rate and long-term survival, but reduce the incidence of OTG-related complications and does not increase the risk of LTG-related complications in stage 0-I gastric cancer. However, these findings still need to be confirmed in a large clinical trial.
Securing Tomorrow's Generation: An Approach Utilizing Machine Learning Models for Children's Online Safety
Authors: Pir Rumel Mohammed Saifur Rahman , Rabbi Md. Forhad , Islam M. Jahirul 
Published: June 2024

Abstract

The increasing usage of online platforms by children in the fast-changing digital environment has raised worries over potential privacy and security hazards. Despite previous study efforts, there are still significant unresolved concerns, particularly the absence of a methodical and all-encompassing approach to comprehending the social safety concerns associated with digital platforms. This involves integrating security and privacy elements with qualitative and categorical data gathered from the viewpoints of children. In order to fill this void, our study undertakes a comprehensive analysis of children's online encounters, collecting firsthand observations from children themselves. Prior to the primary investigation, a preliminary survey was carried out using a cohort of 30 children to authenticate our methodology, hence guiding the development of survey questionnaires. Afterwards, data from a total of 383 children were gathered for analysis. The study develops two models for the dataset—Privacy (Model-I) and Security (Model-II). Employing Principal Component Analysis (PCA) and machine learning algorithms such as Decision Tree, Logistic Regression, Random Forest, and Gaussian Naive Bayes, these models address specific privacy and security concerns. The results unveil intriguing insights into children's perspectives on online privacy and security. In Model-I, 90.1% of children report preserved privacy, with 9.9% reporting violations, while in Model-II, 71.3% of children perceive their security as preserved, with 28.7% reporting violations. Comparing children's perspectives in both models enhances digital safety. This research provides actionable insights from a systematic approach using statistical and machine learning techniques. Our key new finding underscores the importance of considering children's perspectives in policy development for online safety, informing evidence-based interventions and policies. Our systematic approach, built from different techniques, offers a practical and scalable solution for defining privacy and security violations among a vast population of children, enabling policymakers and related child-concerning bodies to easily identify and address such issues across diverse regions and sectors.