bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensory input from the osphradium modulates the response to memory-enhancing stressors in Lymnaea stagnalis
Authors: Karnik Vikram , Braun Marvin , Dalesman Sarah , Lukowiak Ken 
Published: January 2012

SUMMARY
In the freshwater environment species often rely on chemosensory information to modulate behavior. The pond snail, Lymnaea stagnalis, is a model species used to characterize the causal mechanisms of long-term memory (LTM) formation. Chemical stressors including crayfish kairomones and KCl enhance LTM formation (≥24 h) in Lymnaea; however, how these stressors are sensed and the mechanism by which they affect the electrophysiological properties of neurons necessary for memory formation are poorly understood. Here, we assessed whether the osphradium, a primary chemosensory organ in Lymnaea, modulates LTM enhancement. To test this we severed the osphradial nerve proximal to the osphradium, using sham-operated animals as controls, and assessed the behavioral and electrophysiological response to crayfish kairomones and KCl. We operantly conditioned aerial respiratory behavior in intact, sham and osphradially cut animals, and tested for enhanced memory formation after exposure to the chemical stressors. Sham-operated animals displayed the same memory enhancement as intact animals but snails with a severed osphradial nerve did not show LTM enhancement. Extracellular recordings made from the osphradial nerve demonstrate that these stressors evoked afferent sensory activity. Intracellular recordings from right pedal dorsal 1 (RPeD1), a neuron necessary for LTM formation, demonstrate that its electrophysiological activity is altered by input from the osphradium following exposure to crayfish kairomones or KCl in sham and intact animals but no response is seen in RPeD1 in osphradially cut animals. Therefore, sensory input from the osphradium is necessary for LTM enhancement following exposure to these chemical stressors.
Temperature gradients drive mechanical energy gradients in the flight muscle ofManduca sexta
Authors: George N. T. , Sponberg S. , Daniel T. L. 
Published: January 2012

SUMMARYA temperature gradient throughout the dominant flight muscle (dorsolongitudinal muscle, DLM1) of the hawkmoth Manduca sexta, together with temperature-dependent muscle contractile rates, demonstrates that significant spatial variation in power production is possible within a single muscle. Using in situ work-loop analyses under varying muscle temperatures and phases of activation, we show that regional differences in muscle temperature will induce a spatial gradient in the mechanical power output throughout the DLM1. Indeed, we note that this power gradient spans from positive to negative values across the predicted temperature range. Warm ventral subunits produce positive power at their in vivo operating temperatures, and therefore act as motors. Concurrently, as muscle temperature decreases dorsally, the subunits produce approximately zero mechanical power output, acting as an elastic energy storage source, and negative power output, behaving as a damper. Adjusting the phase of activation further influences the temperature sensitivity of power output, significantly affecting the mechanical power output gradient that is expressed. Additionally, the separate subregions of the DLM1 did not appear to employ significant physiological compensation for the temperature-induced differences in power output. Thus, although the components of a muscle are commonly thought to operate uniformly, a significant within-muscle temperature gradient has the potential to induce a mechanical power gradient, whereby subunits within a muscle operate with separate and distinct functional roles.
Warmer is better: thermal sensitivity of both maximal and sustained power output in the iliotibialis muscle isolated from adultXenopus tropicalis
Authors: James Rob S. , Tallis Jason , Herrel Anthony , Bonneaud Camille 
Published: January 2012

SUMMARYEnvironmental temperature varies temporally and spatially and may consequently affect organismal function in complex ways. Effects of temperature are often most pertinent on locomotor performance traits of ectothermic animals. Given the importance of locomotion to mobility and dispersion, variability in temperature may therefore affect the current and future distribution of species. Many previous studies have demonstrated that burst muscle performance changes with temperature. However, less is known about the effects of temperature on sustained skeletal muscle performance. The iliotibialis muscle was isolated from eight male Xenopus tropicalis individuals and subjected to in vitro isometric and work-loop studies at test temperatures of 15, 24, 30 and 32°C. Work-loop power output (average power per cycle) was maximised at each temperature by altering stimulation and strain parameters. A series of 10 work loops was also delivered at each test temperature to quantify endurance performance. Warmer test temperatures tended to increase twitch stress (force normalised to muscle cross-sectional area) and significantly increased tetanic stress. Increased temperature significantly reduced twitch and tetanus activation and relaxation times. Increased temperature also significantly increased both burst muscle power output (cycle average) and sustained (endurance) performance during work loop studies. The increase in burst power output between 15 and 24°C yielded a high Q10 value of 6.86. Recent studies have demonstrated that the negative effects of inorganic phosphate accumulation during prolonged skeletal muscle performance are reduced with increased temperature, possibly explaining the increases in endurance found with increased test temperature in the present study.
Phenotypic plasticity in response to dietary salt stress: Na+ and K+ transport by the gut ofDrosophila melanogasterlarvae
Authors: Naikkhwah Wida , O'Donnell Michael J. 
Published: January 2012

SUMMARYDrosophila provides a useful model system for studies of the mechanisms involved in regulation of internal ion levels in response to variations in dietary salt load. This study assessed whether alterations in Na+ and K+ transport by the gut of larval D. melanogaster reared on salt-rich diets contribute to haemolymph ionoregulation. Na+ and K+ fluxes across the isolated guts of third instar larvae reared on control or salt-rich diets were measured using the scanning ion-selective electrode technique (SIET). K+ absorption across the anterior portion of the posterior midgut of larvae reared on diet in which the concentration of KCl was increased 0.4 mol l-1 above that in the control diet was reduced eightfold relative to the same gut segment of larvae reared on the control diet. There was also an increase in the magnitude and extent of K+ secretion across the posterior half of the posterior midgut. Na+ was absorbed across the ileum of larvae reared on the control diet, but was secreted across the ileum of larvae reared on diet in which the concentration of NaCl was increased 0.4 mol l-1 above that in the control diet. There was also a small reduction in the extent of Na+ absorption across the middle midgut of larvae reared on the NaCl-rich diet. The results indicate considerable phenotypic plasticity with respect to K+ and Na+ transport by the gut epithelia of larval D. melanogaster. SIET measurements of K+ and Na+ fluxes along the length of the gut show that ion transport mechanisms of the gut are reconfigured during salt stress so that there are reductions in K+ and Na+ absorption and increases in K+ and Na+ secretion. Together with previously described changes in salt secretion by the Malpighian tubules, these changes contribute to haemolymph ionoregulation.
Implementation of BioMEMS for Determining Mechanical Properties of Biological Cells
Authors: Tatic-Lucic Svetlana , Gnerlich Markus 
Published: January 2012

ABSTRACTThis paper describes the implementation of a custom-made bio-microelectromechanical system for determining mechanical properties of biological cells, which is used for the measurement of mechanical properties of fibroblasts. Our system consists of several subcomponents: (a) actuator which deforms the cell in pre-determined, step-wise fashion, (b) force sensor that measures force applied onto the cell, (c) set of dielectrophoretic (DEP) electrodes for positioning cells in the desired position, (d) temperature sensors and (e) heater. Preliminary results of the mechanical properties of NIH3T3 cells have been determined using this tool and our cell compression techniques.
Low Profile Packaging for MEMS Aero-acoustic Sensors
Authors: Burns John R. , White Robert D. , Krause Joshua S. 
Published: January 2012

AbstractThis paper describes a semi-automated conductive ink process used for packaging MEMS devices. The method is applied to packaging of MEMS sensors for wind tunnel testing. The primary advantage of the method is a reduction in surface topology between the package and the integrated MEMS sensors. In this paper we explore the relationship between trace dimensions, resistivity, and deposition parameters such as feed rate, tip-substrate separation and tip diameter. Using this procedure it is possible to generate interconnects between a PC board and MEMS sensor chip with a topology of less than 25 micrometers.
Heat-Initiated Oxidation of an Aluminum Nanoparticle
Authors: Clark Richard , Wang Weiqiang , Nomura Ken-ichi , Kalia Rajiv K. , Nakano Aiichiro , Vashishta Priya 
Published: January 2012

ABSTRACTMultimillion-atom reactive molecular dynamics simulations were used to investigate the mechanisms which control heat-initiated oxidation in aluminum nanoparticles. The simulation results reveal three stages: (1) confined burning, (2) onset of deformation, and (3) onset of small cluster ejections. The first stage of the reaction is localized primarily at the core-shell boundary, where oxidation reactions result in strong local heating and the increased migration of oxygen from the shell into the core. When the local temperature rises above the melting point of alumina (T=2330K), the melting of the shell allows deformation of the overall particle and an increase in heat production. Finally, once the particle temperature exceeds 2800-3000 K, small aluminum-rich clusters are ejected from the outside of the shell. The underlying mechanisms were explored using global and radial statistical analysis, as well as developed visualization techniques and localized fragment analysis.The three-stage reaction mechanism found here provides insight into the controlling factors of aluminum nanoparticle oxidation, a topic of considerable importance in the energetic materials community.
Resistance Change Caused by Electrochemically Induced Carrier Injection in NiO Films.
Authors: Yoda T. , Kinoshita K. , Fukuhara T. , Kishida S. , Sawai N. , Honda K. 
Published: January 2012

ABSTRACTIn our previous work, low resistance state (LRS) and high resistance state (HRS) areas on a nickel-oxide (NiO) film formed by applying a voltage using conductive atomic-force microscopy (C-AFM) was observed by scanning electron microscope (SEM). Comparing the observed secondary electron image (SEI) contrast to the report about the dopant-type dependence of SEI contrast reported on silicon, it was suggested that the LRS and HRS areas are, respectively, electrochemically induced p-type Ni1-xO (x > 0) and intrinsic (stoichiometric) or ntype Ni1-xO (x ≤ 0). In this paper, we verified that resistance change caused by C-AFM is due to electrochemically induced carrier injection. Reduction effect of H2annealing on the writing area, voltage dependence of depletion layer capacitance formed between the writing area and AFM-tip using scanning nonlinear dielectric microscopy (SNDM), and the effect of Schottky barrier formation between the writing area and thin metal layer on SEI contrast were investigated. Based on these results, it was clarified that the LRS and HRS areas are, respectively, p-type Ni1-xO (x > 0) and intrinsic (stoichiometric) or n-type Ni1-xO (x ≤ 0)
Mortality Experience of a Low-Income Population With Young-Onset Diabetes
Authors: Conway Baqiyyah N. , May Michael E. , Signorello Lisa B. , Blot William J. 
Published: January 2012

OBJECTIVE
In young-onset diabetes, insulin therapy status is a rough marker of diabetes type. We describe the mortality experience of a low-income, predominantly minority population with diabetes diagnosed before age 30 years, stratified by insulin therapy.

RESEARCH DESIGN AND METHODS
A total of 1,098 adults aged 40–79 years (median 49) diagnosed with diabetes before age 30 years and 49,914 without diabetes were recruited from community health centers. Individuals with diabetes were categorized by insulin therapy at baseline: group A, insulin therapy only; group B, insulin therapy and an oral hypoglycemic agent; and group C, no insulin therapy. Cox models were used to compute hazard ratios (HRs) and 95% CI for cause-specific mortality based on both underlying and contributing causes of death from death certificates.

RESULTS
During follow-up (mean 3.9 years), 15.0, 12.5, and 7.3% of groups A, B, and C, respectively, and 4.6% without diabetes died. Compared with individuals without diabetes, HRs (CI) for all-cause mortality were 4.3 (3.4–5.6), 4.2 (2.8–6.3), and 2.0 (1.4–2.8) in groups A, B, and C, respectively. The leading cause of death was renal failure (end-stage renal disease [ESRD]) in group A, ESRD and coronary artery disease (CAD) in group B, and CAD in group C and individuals without diabetes. HRs for these conditions were at least twice as high as the HRs for all-cause mortality, reaching 17.3 (10.2–29.3), 17.9 (8.3–38.7), and 5.1 (2.3–11.7) in groups A, B, and C, respectively, for ESRD.

CONCLUSIONS
Excess mortality persists among people with young-onset diabetes of long duration, with ESRD and CAD as the leading contributors to mortality.
Novel Urinary Protein Biomarkers Predicting the Development of Microalbuminuria and Renal Function Decline in Type 1 Diabetes
Authors: Schlatzer Daniela , Maahs David M. , Chance Mark R. , Dazard Jean-Eudes , Li Xiaolin , Hazlett Fred , Rewers Marian , Snell-Bergeon Janet K. 
Published: January 2012

OBJECTIVE
To define a panel of novel protein biomarkers of renal disease.

RESEARCH DESIGN AND METHODS
Adults with type 1 diabetes in the Coronary Artery Calcification in Type 1 Diabetes study who were initially free of renal complications (n = 465) were followed for development of micro- or macroalbuminuria (MA) and early renal function decline (ERFD, annual decline in estimated glomerular filtration rate of ≥3.3%). The label-free proteomic discovery phase was conducted in 13 patients who progressed to MA by the 6-year visit and 11 control subjects, and four proteins (Tamm-Horsfall glycoprotein, α-1 acid glycoprotein, clusterin, and progranulin) identified in the discovery phase were measured by enzyme-linked immunosorbent assay in 74 subjects: group A, normal renal function (n = 35); group B, ERFD without MA (n = 15); group C, MA without ERFD (n = 16); and group D, both ERFD and MA (n = 8).

RESULTS
In the label-free analysis, a model of progression to MA was built using 252 peptides, yielding an area under the curve (AUC) of 84.7 ± 5.3%. In the validation study, ordinal logistic regression was used to predict development of ERFD, MA, or both. A panel including Tamm-Horsfall glycoprotein (odds ratio 2.9, 95% CI 1.3–6.2, P = 0.008), progranulin (1.9, 0.8–4.5, P = 0.16), clusterin (0.6, 0.3–1.1, P = 0.09), and α-1 acid glycoprotein (1.6, 0.7–3.7, P = 0.27) improved the AUC from 0.841 to 0.889.

CONCLUSIONS
A panel of four novel protein biomarkers predicted early renal damage in type 1 diabetes. These findings require further validation in other populations for prediction of renal complications and treatment monitoring.
Diabetes, Its Treatment, and Catastrophic Medical Spending in 35 Developing Countries
Authors: Smith-Spangler Crystal M. , Bhattacharya Jay , Goldhaber-Fiebert Jeremy D. 
Published: January 2012

OBJECTIVE
To assess the individual financial impact of having diabetes in developing countries, whether diabetic individuals possess appropriate medications, and the extent to which health insurance may protect diabetic individuals by increasing medication possession or decreasing the risk of catastrophic spending.

RESEARCH DESIGN AND METHODS
Using 2002–2003 World Health Survey data (n = 121,051 individuals; 35 low- and middle-income countries), we examined possession of medications to treat diabetes and estimated the relationship between out-of-pocket medical spending (2005 international dollars), catastrophic medical spending, and diabetes. We assessed whether health insurance modified these relationships.

RESULTS
Diabetic individuals experience differentially higher out-of-pocket medical spending, particularly among individuals with high levels of spending (excess spending of $157 per year [95% CI 130–184] at the 95th percentile), and a greater chance of incurring catastrophic medical spending (17.8 vs. 13.9%; difference 3.9% [95% CI 0.2–7.7]) compared with otherwise similar individuals without diabetes. Diabetic individuals with insurance do not have significantly lower risks of catastrophic medical spending (18.6 vs. 17.7%; difference not significant), nor were they significantly more likely to possess diabetes medications (22.8 vs. 20.6%; difference not significant) than those who were otherwise similar but without insurance. These effects were more pronounced and significant in lower-income countries.

CONCLUSIONS
In low-income countries, despite insurance, diabetic individuals are more likely to experience catastrophic medical spending and often do not possess appropriate medications to treat diabetes. Research into why policies in these countries may not adequately protect people from catastrophic spending or enhance possession of critical medications is urgently needed.
25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth
Authors: Rajakumar Kumaravel , de las Heras Javier , Lee SoJung , Holick Michael F. , Arslanian Silva A. 
Published: January 2012

OBJECTIVE
To examine the relationships between plasma 25-hydroxyvitamin D [25(OH)D] and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity, disposition index (DI), in black and white youth.

RESEARCH DESIGN AND METHODS
Plasma 25(OH)D concentrations were analyzed in banked specimens in healthy youth aged 8 to 18 years who had existing data on hyperinsulinemic-euglycemic and hyperglycemic clamp to assess insulin sensitivity and secretion, and measurements of body composition, and abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT).

RESULTS
A total of 183 research volunteers (mean ± SD; age, 12.6 ± 2.2 years; 98 white, 98 male, 92 obese) were studied. Analysis of HbA1c, fasting glucose and insulin, insulin sensitivity, and DI across quartiles of plasma 25(OH)D revealed no differences among whites. In blacks, the observed significance of higher insulin sensitivity and DI in the highest quartile of 25(OH)D disappeared after adjusting for any of the adiposity measures (BMI or fat mass or VAT or SAT). The difference in insulin sensitivity (9.4 ± 1.2 vs. 5.6 ± 0.5 mg/kg/min per μU/mL; P = 0.006) between 25(OH)D nondeficient (≥20 ng/mL) versus deficient (&lt;20 ng/mL) black youth also was negated when adjusted for adiposity.

CONCLUSIONS
In healthy youth, plasma 25(OH)D concentrations bear no independent relationship to parameters of glucose homeostasis and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity. It remains to be determined whether in youth with dysglycemia the relationships are different and whether vitamin D optimization enhances insulin sensitivity and β-cell function.
Critical issues in contemporary education: Prolegomena
Authors: Kaščák Ondrej , Pupala Branislav 
Published: January 2012

Abstract
This paper summarizes the problematic aspects of a globalized neoliberal culture in education. Linking to the particular studies of this monothematic volume it discusses the consequences of the globalization of a testing culture in schools, the issues of developing civic literacy in the context of current education practice and the issues of forming a historic consciousness in present schools relating to the existing social discourse. Language teaching, currently dominated by the concept of language literacy or the concept of language education resulting from English language teaching seems to be significant. This paper reveals various ways in which the educational section is being contaminated by neoliberal transformations to point out their culturally devastating consequences in a critical way. The goal of this paper is to articulate the mechanism by which neoliberalism is infiltrating education in the form of discursive and physical “colonizing”.
Educating for profit, educating global citizenship
Authors: Hickman Larry 
Published: January 2012

Abstract
After reviewing current proposals for standardized testing in K-12 education (United States) and for imposition of free-market economic and business models on higher education (Texas, Florida, and the United Kingdom), I argue that both types of proposals rest on flawed pedagogical assumptions and tend to undermine educational practices that promote the development of global citizens. I suggest that John Dewey was aware of the type of challenges now faced by educators and that he provided tools for blunting the force of these proposals and moving educational practice toward more desirable ends.
Influence of Treatment with Intranasal Corticosteroids on the Nasal Mucosa, Weight, and Corticosteroid Concentration in Rats
Authors: Park Danielle Morello , Carvalho Teresa Lúcia Lamano , Franci Janete Aparecida Anselmo , Anselmo-Lima Wilma Terezinha 
Published: January 2012

Background The effect of intranasal corticosteroids on the nasal epithelium mucosa is an important parameter of treatment safety. This study was designed to examine whether treatment with topical corticosteroids in patients with allergic rhinitis causes atrophic nasal mucosal changes, when compared with systemic corticosteroids, in rats. Methods Male Wistar rats were treated daily during 7 weeks with topical administration with 10 microliters of normal saline (control group), 10 microliters of mometasone furoate group, 10 microliters of triamcinolone acetonide (T group), and 8 mg/kg of daily subcutaneous injections of methylprednisolone sodium succinate (MP group). Body weight was evaluated weekly. At the end of the treatment, rats were killed by decapitation to collect blood for determination of corticosterone levels and nasal cavities were prepared for histological descriptive analyses. Results Treatment with T and MP decreased body weight. Plasma corticosterone concentration was significantly reduced by MP treatment and presented a clear tendency to decrease after T treatment. Histological changes observed in group T included ripples, cell vacuolization, increase in the number of nuclei, and decrease in the number of cilia in the epithelial cells. Conclusion Growth and corticosterone concentration were impaired by T and MP at the same proportion, suggesting a role of this hormone in body gain. With the exception of T, intranasal or systemic treatment with the corticosteroids evaluated in this study did not affect nasal mucosa.