bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective fertilization in interspecific crosses of allotetraploid species of Gossypium
Authors: Pereira G.S. , Sousa R.L. , Araújo R.L. , Hoffmann L.V. , Silva E.F. , Barroso P.A.V. 
Published: March 2012

The genus Gossypium is composed of both diploid and allotetraploid species. The five allotetraploid species of Gossypium are sexually compatible, and only partial sexual barriers have been described. Natural hybrids among them do not occur or occur in situ with very low frequency in Brazil in the rare places where cultivated upland cotton ( Gossypium hirsutum L.) occurs in a sympatric range with Gossypium barbadense L. or Gossypium mustelinum Miers. We evaluated the presence of pollen competition as a prezygotic barrier in crosses between upland cotton and G. barbadense and G. mustelinum. We found that G. barbadense pollinated with a mixture of 50% upland cotton and 50% G. barbadense pollen resulted in 17.4%–31.1% interspecific hybrids, depending upon the upland cotton genotype used as pollen donor. Mixtures containing pollen from G. mustelinum and upland cotton, used to pollinate G. mustelinum in proportions of 25%:75%, 50%:50%, and 75%:25%, produced 61.3%, 22.5%, and 3.6% interspecific hybrids, respectively. These low rates of hybrid production demonstrate that pollen competition is present with G. mustelinum and G. barbadense and confirms this mechanism as a sexual barrier.
Calcul de composition de plasmas thermiques d’arc électrique de mélanges d’air et de vapeur d’eau
Authors: Kagoné Abdoul Karim , Koalaga Zacharie , Zougmoré François 
Published: March 2012

La composition de plasmas thermiques d’arcs électriques de mélanges d’air et de vapeur d’eau dans une gamme de température électronique allant de 5000 à 30 000 K est calculée pour des pressions de 1, 5 et 10 atm (1 atm = 101,325 kPa). Les plasmas étudiés étant considérés hors d’équilibre thermique, le calcul de la composition est effectué pour des déséquilibres thermiques θ = 1, 1,2 et 1,5 par deux méthodes de calcul à savoir celles de Chen et de Potapov. Le déséquilibre thermique θ est défini comme suit : θ = Te/Th où Te représente la température cinétique des électrons et Th celle des particules lourdes (molécules, atomes et ions). Les résultats obtenus sont analysés et discutés en fonction des méthodes de calcul utilisées, du déséquilibre thermique, de la pression et de la proportion initiale de la vapeur d’eau dans le mélange ayant donné naissance au plasma. L’influence de la méthode de calcul à deux températures, du déséquilibre thermique et de la pression sur la composition chimique des plasmas des mélanges étudiés est mise en évidence. Les résultats du calcul de la composition montrent en particulier que la densité numérique d’atomes d’hydrogène croît bien avec la proportion en vapeur d’eau dans le mélange.
Characterization and structural studies of vanadium doped lithium–barium–phosphate glasses
Authors: Lakshmikantha R. , Rajaramakrishna R. , Anavekar R.V. , Ayachit N.H. 
Published: March 2012

Characterization and structural studies of (P2O5)49.5(V2O5)0.5(BaO)x(Li2O)50–x (where x = 0, 5, 10, 15, 20, 25 mol%) glasses are reported. These glasses were prepared using the conventional melt quenching technique. X-ray diffraction and differential scanning calorimetry measurements were performed to confirm their amorphous nature. Infrared spectra of these glasses show absorption peaks in the ranges 1273–1267; 1098–1088; 1008–1018; 899–889; and 795–760 and 506–474 cm–1 corresponding to P=O, P–O–, (P–O–P)S, (P–O–P)AS, and O–P–O group vibrations, respectively. Thermal stability factor (s) has been evaluated using the values of Tg and Tc. These glasses exhibit high transition temperature (Tg) and high thermal stability factor (s) indicating that they are thermally stable. Refractive index of the glasses lies in the range 1.59–1.49. Electron paramagnetic resonance spectroscopy of V4+ show characteristic hyperfine split signal arising from the vanadyl group in this glass matrix. The results are discussed by considering the structural modification of the phosphate network.
Effect of deposition conditions on physical properties of SnO2 thin films prepared using the spray pyrolysis technique
Authors: Memarian N. , Rozati S.M. 
Published: March 2012

In this study, physical properties of tin oxide thin films, which are prepared using the spray pyrolysis technique, have been analyzed. The effects of substrate temperature and precursor molarity on optoelectrical and structural properties of the films have been investigated. X-ray diffraction analysis revealed a polycrystalline nature with tetragonal structure. It also showed that the preferential growth directions of the films were dependent on the deposition conditions. By using scanning electron microscopy analysis, the surface morphology of the films was observed as an effect of the variation of deposition conditions. The optical and electrical properties of the films were studied by transmission spectra and Hall effect measurement, respectively. Films of about 6 × 10−3 Ω cm resistivity and high visible transparency of about 86% have been obtained at the optimum substrate temperature of 550 °C and precursor molarity of 0.3 mol/L.
Charge transfer and ionization in collisions of O8+ ions with excited He atoms
Authors: Wang Fei , Wang Miao-miao 
Published: March 2012

Two-centre atomic orbital close-coupling calculations are carried out for the cross sections of single electron capture and single ionization in O8+ + He(1s1s) and O8+ + He(1s3d) collisions in the velocity range from 0.6 to 2.0 a.u. The relative importance of single ionization to single capture are investigated for both systems. Our calculations are in good agreement with experimental data of O8+ + He(1s1s) collision. The ratio curves also show us distinct behavior between the two collision systems. The partial electron capture cross sections for O8+ + He(1s3d) collision indicate that, corresponding with the astrophysical conclusion, most of the l-levels are populated statistically for energies greater than 10 keV, and some distributions of lower n at low collision energy show a trend close to the binomial distribution.
Defining NOTCH3 Target Genes in Ovarian Cancer
Authors: Chen Xu , Thiaville Michelle M. , Chen Li , Stoeck Alexander , Xuan Jianhua , Gao Min , Shih Ie-Ming , Wang Tian-Li 
Published: March 2012

AbstractNOTCH3 gene amplification plays an important role in the progression of many ovarian and breast cancers, but the targets of NOTCH3 signaling are unclear. Here, we report the use of an integrated systems biology approach to identify direct target genes for NOTCH3. Transcriptome analysis showed that suppression of NOTCH signaling in ovarian and breast cancer cells led to downregulation of genes in pathways involved in cell-cycle regulation and nucleotide metabolism. Chromatin immunoprecipitation (ChIP)-on-chip analysis defined promoter target sequences, including a new CSL binding motif (N1) in addition to the canonical CSL binding motif, that were occupied by the NOTCH3/CSL transcription complex. Integration of transcriptome and ChIP-on-chip data showed that the ChIP target genes overlapped significantly with the NOTCH-regulated transcriptome in ovarian cancer cells. From the set of genes identified, we showed that the mitotic apparatus organizing protein DLGAP5 (HURP/DLG7) was a critical target. Both the N1 motif and the canonical CSL binding motif were essential to activate DLGAP5 transcription. DLGAP5 silencing in cancer cells suppressed tumorigenicity and inhibited cellular proliferation by arresting the cell cycle at the G2–M phase. In contrast, enforced expression of DLGAP5 partially counteracted the growth inhibitory effects of a pharmacologic or RNA interference–mediated NOTCH inhibition in cancer cells. Our findings define direct target genes of NOTCH3 and highlight the role of DLGAP5 in mediating the function of NOTCH3. Cancer Res; 72(9); 2294–303. ©2012 AACR.
Impact of Intertumoral Heterogeneity on Predicting Chemotherapy Response of BRCA1-Deficient Mammary Tumors
Authors: Rottenberg Sven , Vollebergh Marieke A. , de Hoon Bas , de Ronde Jorma , Schouten Philip C. , Kersbergen Ariena , Zander Serge A.L. , Pajic Marina , Jaspers Janneke E. , Jonkers Martijn , Lodén Martin , Sol Wendy , van der Burg Eline , Wesseling Jelle , Gillet Jean-Pierre , Gottesman Michael M. , Gribnau Joost , Wessels Lodewyk , Linn Sabine C. , Jonkers Jos , Borst Piet 
Published: March 2012

Abstract
The lack of markers to predict chemotherapy responses in patients poses a major handicap in cancer treatment. We searched for gene expression patterns that correlate with docetaxel or cisplatin response in a mouse model for breast cancer associated with BRCA1 deficiency. Array-based expression profiling did not identify a single marker gene predicting docetaxel response, despite an increase in Abcb1 (P-glycoprotein) expression that was sufficient to explain resistance in several poor responders. Intertumoral heterogeneity explained the inability to identify a predictive gene expression signature for docetaxel. To address this problem, we used a novel algorithm designed to detect differential gene expression in a subgroup of the poor responders that could identify tumors with increased Abcb1 transcript levels. In contrast, standard analytical tools, such as significance analysis of microarrays, detected a marker only if it correlated with response in a substantial fraction of tumors. For example, low expression of the Xist gene correlated with cisplatin hypersensitivity in most tumors, and it also predicted long recurrence-free survival of HER2-negative, stage III breast cancer patients treated with intensive platinum-based chemotherapy. Our findings may prove useful for selecting patients with high-risk breast cancer who could benefit from platinum-based therapy. Cancer Res; 72(9); 2350–61. ©2012 AACR.
CD73-Deficient Mice Are Resistant to Carcinogenesis
Authors: Stagg John , Beavis Paul A. , Divisekera Upulie , Liu Mira C.P. , Möller Andreas , Darcy Phillip K. , Smyth Mark J. 
Published: March 2012

Abstract
CD73 is a cell surface 5′-nucleotidase that converts AMP to adenosine, an immune suppressive molecule. CD73 may promote immune escape in cancer by contributing to the degradation of extracellular ATP released by dying cancer cells in hypoxic tumors or following chemotherapy. However, whether CD73 exerts a critical oncogenic function during tumorigenesis is unknown. In this study, we used genetically deficient mice to investigate its contribution to autochthonous tumor formation. CD73 deficiency suppressed the development of 3-methylcholanthrene (MCA)-induced fibrosarcomas through a mechanism relying upon IFN-γ, natural killer (NK) cells, and CD8+ T cells. Similarly, CD73 deficiency also suppressed prostate tumorigenesis in TRAMP transgenic mice. Importantly, treatment with an anti-CD73 monoclonal antibody effectively suppressed growth of established MCA-induced tumors or TRAMP-C1 prostate tumors and inhibited the development of TRAMP-C1 lung metastases. The therapeutic activity of anti-CD73 monoclonal antibody against primary tumors was dependent on CD8+ T cells, whereas its antimetastatic activity was dependent on host CD73 expression independent of T cells or NK cells. Taken together, our findings indicate that CD73 is a critical factor in tumorigenesis and that anti-CD73 antibodies may offer a novel generalized strategy to blunt immune escape and treat cancer. Cancer Res; 72(9); 2190–6. ©2012 AACR.
Urinary Bladder Cancer Risk Factors in Egypt: A Multicenter Case–Control Study
Authors: Zheng Yun-Ling , Amr Sania , Saleh Doa'a A. , Dash Chiranjeev , Ezzat Sameera , Mikhail Nabiel N. , Gouda Iman , Loay Iman , Hifnawy Tamer , Abdel-Hamid Mohamed , Khaled Hussein , Wolpert Beverly , Abdel-Aziz Mohamed A. , Loffredo Christopher A. 
Published: March 2012

Abstract
Background: We investigated associations between tobacco exposure, history of schistosomiasis, and bladder cancer risk in Egypt.
Methods: We analyzed data from a case–control study (1,886 newly diagnosed and histologically confirmed cases and 2,716 age-, gender-, and residence-matched, population-based controls). Using logistic regression, we estimated the covariate-adjusted ORs and 95% confidence interval (CI) of the associations.
Results: Among men, cigarette smoking was associated with an increased risk of urothelial carcinoma (OR = 1.8; 95% CI, 1.4–2.2) but not squamous cell carcinoma (SCC); smoking both water pipes and cigarettes was associated with an even greater risk for urothelial carcinoma (OR = 2.9; 95% CI, 2.1–3.9) and a statistically significant risk for SCC (OR = 1.8; 95% CI, 1.2–2.6). Among nonsmoking men and women, environmental tobacco smoke exposure was associated with an increased risk of urothelial carcinoma. History of schistosomiasis was associated with increased risk of both urothelial carcinoma (OR = 1.9; 95% CI, 1.2–2.9) and SCC (OR = 1.9; 95% CI, 1.2–3.0) in women and to a lesser extent (OR = 1.4; 95% CI, 1.2–1.7 and OR = 1.4; 95% CI, 1.1–1.7, for urothelial carcinoma and SCC, respectively) in men.
Conclusions: The results suggest that schistosomiasis and tobacco smoking increase the risk of both SCC and urothelial carcinoma.
Impact: This study provides new evidence for associations between bladder cancer subtypes and schistosomiasis and suggests that smoking both cigarettes and water pipes increases the risk for SCC and urothelial carcinoma in Egyptian men. Cancer Epidemiol Biomarkers Prev; 21(3); 537–46. ©2011 AACR.
Gene Set Analysis of Survival Following Ovarian Cancer Implicates Macrolide Binding and Intracellular Signaling Genes
Authors: Fridley Brooke L. , Jenkins Gregory D. , Tsai Ya-Yu , Song Honglin , Bolton Kelly L. , Fenstermacher David , Tyrer Jonathan , Ramus Susan J. , Cunningham Julie M. , Vierkant Robert A. , Chen Zhihua , Chen Y. Ann , Iversen Ed , Menon Usha , Gentry-Maharaj Aleksandra , Schildkraut Joellen , Sutphen Rebecca , Gayther Simon A. , Hartmann Lynn C. , Pharoah Paul D.P. , Sellers Thomas A. , Goode Ellen L. 
Published: March 2012

AbstractBackground: Genome-wide association studies (GWAS) for epithelial ovarian cancer (EOC), the most lethal gynecologic malignancy, have identified novel susceptibility loci. GWAS for survival after EOC have had more limited success. The association of each single-nucleotide polymorphism (SNP) individually may not be well suited to detect small effects of multiple SNPs, such as those operating within the same biologic pathway. Gene set analysis (GSA) overcomes this limitation by assessing overall evidence for association of a phenotype with all measured variation in a set of genes.Methods: To determine gene sets associated with EOC overall survival, we conducted GSA using data from two large GWAS (N cases = 2,813, N deaths = 1,116), with a novel Principal Component-Gamma GSA method. Analysis was completed for all cases and then separately for high-grade serous histologic subtype.Results: Analysis of the high-grade serous subjects resulted in 43 gene sets with P &lt; 0.005 (1.7%); of these, 21 gene sets had P &lt; 0.10 in both GWAS, including intracellular signaling pathway (P = 7.3 × 10−5) and macrolide binding (P = 6.2 × 10−4) gene sets. The top gene sets in analysis of all cases were meiotic mismatch repair (P = 6.3 × 10−4) and macrolide binding (P = 1.0 × 10−3). Of 18 gene sets with P &lt; 0.005 (0.7%), eight had P &lt; 0.10 in both GWAS.Conclusion: This research detected novel gene sets associated with EOC survival.Impact: Novel gene sets associated with EOC survival might lead to new insights and avenues for development of novel therapies for EOC and pharmacogenomic studies. Cancer Epidemiol Biomarkers Prev; 21(3); 529–36. ©2012 AACR.
Associations between Genes in the One-Carbon Metabolism Pathway and Advanced Colorectal Adenoma Risk in Individuals with Low Folate Intake
Authors: Han Summer S. , Sue Laura Y. , Berndt Sonja I. , Selhub Jacob , Burdette Laurie A. , Rosenberg Philip S. , Ziegler Regina G. 
Published: March 2012

Abstract
Background: Folate is essential for one-carbon metabolism, a pathway required by DNA synthesis, methylation, and repair. Low dietary and circulating folate and polymorphic variation in this pathway are associated with increased risk of colorectal adenoma and cancer.
Methods: We genotyped 882 single nucleotide polymorphisms (SNP) in 82 one-carbon metabolism genes for 1,331 cases of advanced colorectal adenoma, identified by sigmoidoscopy at baseline, and 1,501 controls from the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO). We evaluated associations between one-carbon genes and adenoma risk in all subjects and stratified by folate intake. We applied the Adaptive Rank Truncated Product (ARTP) method to assess statistical significance at the gene and pathway levels.
Results: Folate intake was inversely associated with advanced colorectal adenoma risk [odds ratio (OR) by quartile = 0.85, P = 1.9 × 10−5]. We found no statistically significant associations between one-carbon genes and adenoma risk in all subjects. As hypothesized, we observed a statistically significant pathway-level association (P = 0.038) in the lowest quartile of folate; no significant associations were found in higher quartiles. Several genes including adenosine deaminase (ADA) and cysteine dioxygenase (CDO1) contributed to this signal (gene-level P = 0.001 and 0.0073, respectively). The most statistically significant SNP was rs244072 in ADA (P = 2.37 × 10−5).
Conclusions and Impact: Stratification by dietary folate and application of the ARTP method revealed statistically significant pathway- and gene-level associations between one-carbon metabolism genes and risk of advanced colorectal adenoma, which were not apparent in analysis of the entire population. Folate intake may interact with associations between common variants in one-carbon metabolism genes and colorectal adenoma risk. Cancer Epidemiol Biomarkers Prev; 21(3); 417–27. ©2012 AACR.
Vitamin D and Calcium Supplementation and One-Year Change in Mammographic Density in the Women's Health Initiative Calcium and Vitamin D Trial
Authors: Bertone-Johnson Elizabeth R. , McTiernan Anne , Thomson Cynthia A. , Wactawski-Wende Jean , Aragaki Aaron K. , Rohan Thomas E. , Vitolins Mara Z. , Tamimi Rulla M. , Johnson Karen C. , Lane Dorothy , Rexrode Kathryn M. , Peck Jennifer D. , Chlebowski Rowan T. , Sarto Gloria , Manson JoAnn E. 
Published: March 2012

AbstractBackground: Calcium and vitamin D may be inversely related to breast cancer risk, in part by affecting mammographic density. However, results from previous, mostly cross-sectional studies have been mixed, and there have been few randomized clinical trials of the effect of calcium and vitamin D supplementation on change in mammographic density.Methods: We assessed the effect of one year of supplementation on mammographic density in 330 postmenopausal women enrolled in the Women's Health Initiative hormone therapy (HT) and calcium and vitamin D (CaD) trials. Women were randomized to receive 1,000 mg/d of elemental calcium carbonate plus 400 IU/d of vitamin D3 or placebo.Results: After approximately one year, mammographic density decreased 2% in the CaD supplementation group and increased 1% in the placebo group (ratio of means = 0.97; 95% CI = 0.81–1.17). Results suggested potential interaction by HT use (P = 0.08). Among women randomized to HT placebo, the ratio of mean density comparing CaD supplementation and placebo groups was 0.82 (95% CI = 0.61–1.11) vs. 1.16 (95% CI = 0.92–1.45) in women randomized to active HT. In sensitivity analyses limited to women taking ≥80% of study supplements, ratios were 0.67 (95% CI = 0.41–1.07) in women not assigned to HT and 1.07 (95% CI = 0.79–1.47) women assigned to HT.Conclusions: We observed no overall effect of vitamin D and calcium supplementation on mammographic density after one year.Impact: Potential interaction between these nutrients and estrogen as related to mammographic density warrants further study. Cancer Epidemiol Biomarkers Prev; 21(3); 462–73. ©2012 AACR.
The Role of Polymorphisms in DNA Repair Genes and HPV 18 Integration Status in Cervical Dysplasia
Authors: ES Amirian , D Marquez-Do , K Adler-Storthz , M Follen , ME Scheurer 
Published: March 2012

Abstract
Despite the fact that cervical cancer is one of the leading causes of death in women worldwide, research has yet to elucidate why some HPV-infected women develop cancerous lesions while others are able to clear the infection. Previous studies have shown that HPV integration status may be associated with cervical cancer development, and yet, host genetic factors that may be involved in the viral integration process have not yet been identified. The purpose of this study was to examine the association between both HPV 18 viral integration status and single nucleotide polymorphisms (SNPs) in non-homologous end-joining (NHEJ) DNA repair pathway genes on cervical dysplasia. Specifically, we sought to compare women with no dysplasia to those with low-grade or high-grade squamous intraepithelial lesions.
Methods: A total of 765 women were selected from two large trials designed to evaluate optical technologies for cervical cancer. Genotyping was performed using the Illumina Golden Gate platform. HPV 18 integration status was determined using a previously established protocol. Chi-square tests were conducted to determine which SNPs were associated with normal cytology, low-grade, or high-grade lesions. Among participants with cervical dysplasia, polytomous logistic regression models were used to evaluate the effect of each polymorphism on viral integration status. An additive genetic model was used for all tests. P-values were adjusted using the false discovery rate method.
Results: Women with high-grade lesions were significantly younger than women with low-grade or no lesions. Tag-SNPs in 13 DNA repair genes, including MRE11A, ATM, and XRCC4, were significantly associated with cervical dysplasia. Most participants had a mix of both episomal and integrated HPV 18. Tag-SNPs in the XRCC4, PRKCH, and MRE11A genes were found to be significantly associated with HPV 18 integration status.
Conclusion: Our study indicates that host genetic variation in NHEJ DNA repair pathway genes, including MRE11A and XRCC4, are significantly associated with HPV 18 integration, and that these genes may play a key role in determining cervical cancer development and progression. This is the first study to examine host genetic variation in association with the viral integration event.
Ruminant Fatty Acids and Prostate Cancer Risk in the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study
Authors: M Wright , D Albanes , A Moser , K Snyder , J Virtamo , P Gann 
Published: March 2012

Abstract
Consumption of high fat animal products is a characteristic feature of Western diets, which have been consistently linked with elevated risks of prostate cancer. In order to elucidate which specific fatty acids may contribute to this association, we measured circulating concentrations of myristic (C14:0), pentadecanoic (C15:0), palmitic (C16:0), heptadecanoic (C17:0), vaccenic (C18:1n-7), and alpha-linolenic (C18:3n-3) acids - all of which are present in ruminant meat and/or dairy products - in a nested case-control study within the Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study. Prediagnostic blood samples from 300 prostate cancer cases and 300 controls matched on age and date of serum blood draw were analyzed for the aforementioned fatty acids by gas chromatography-mass spectrometry. There was a positive association between serum alpha-linolenic acid and overall prostate cancer risk, with a notable threshold effect (for increasing quartiles, odds ratios and 95% confidence intervals = 1.0 (referent), 1.65 (1.02–2.67), 1.96 (1.22–3.14), and 1.57 (0.97–2.54); p trend = 0.15). This association was stronger among men with low baseline levels of beta-carotene and vitamin E. The other fatty acids were unrelated to prostate cancer risk. Our findings indicate that higher blood levels of alpha-linolenic acid - the predominant omega-3 fatty acid in Western diets - is associated with elevated risks of prostate cancer, particularly within subgroups of men with low antioxidant levels.