bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutral interactions between native Alternanthera sessilis and exotic Alternanthera philoxeroides along a light gradient under tropical climatic conditions
Authors: Kankanamge Champika Ellawala , Sujaw M. N. Nusky 
Published: May 2024

AbstractRiparian ecosystems are under a serious threat of invasion due to the clearance of riparian vegetation, which allows for increased light levels at ground level. This study presents the interactions between native Alternanthera sessilis (L.) R. Br. ex DC and invasive Alternanthera philoxeroides (Mart.) Griseb. during the colonization stage along a light gradient under tropical climate conditions. We conducted a 6 week growth experiment with monocultures of A. philoxeroides and A.sessilis and mixed communities of two species under four light levels (100%, 46%, 26% and 14% of full sunlight). Exposure to full sunlight (nearly 1800 μmol m−2 s−1) resulted in a significantly high biomass accrual in both species. However, biomass accrual did not correlate with the light intensity. At higher shade levels, reduction of lateral spread and shoot/root ratio was observed. Community composition had no influence on biomass accrual or morphological variations. Plasticity index values were approximately same in both species. Total relative yield was approximately two in all shade conditions, indicating that the two species interact neutrally along the light gradient. Therefore, the results of the study indicate that increased light levels will accelerate the proliferation of both species in riparian zones. Nevertheless, increased or decreased shade is unlikely to alter neutral interactions between A. philoxeroides and A. sessilis.
Stabilization distance bounds from link Floer homology
Authors: Juhász András , Zemke Ian 
Published: May 2024

AbstractWe consider the set of connected surfaces in the 4‐ball with boundary a fixed knot in the 3‐sphere. We define the stabilization distance between two surfaces as the minimal such that we can get from one to the other using stabilizations and destabilizations through surfaces of genus at most . Similarly, we consider a double‐point distance between two surfaces of the same genus that is the minimum over all regular homotopies connecting the two surfaces of the maximal number of double points appearing in the homotopy. To many of the concordance invariants defined using Heegaard Floer homology, we construct an analogous invariant for a pair of surfaces. We show that these give lower bounds on the stabilization distance and the double‐point distance. We compute our invariants for some pairs of deform‐spun slice disks by proving a trace formula on the full infinity knot Floer complex, and by determining the action on knot Floer homology of an automorphism of the connected sum of a knot with itself that swaps the two summands. We use our invariants to find pairs of slice disks with arbitrarily large distance with respect to many of the metrics we consider in this paper. We also answer a slice‐disk analog of Problem 1.105 (B) from Kirby's problem list by showing the existence of non‐0‐cobordant slice disks.
Symposium review: high altitude travel with pulmonary vascular disease
Authors: Titz Anna , Schneider Simon , Mueller Julian , Mayer Laura , Lichtblau Mona , Ulrich Silvia 
Published: May 2024

AbstractPulmonary arterial hypertension and chronic thromboembolic pulmonary hypertension are the main precapillary forms of pulmonary hypertension (PH) summarized as pulmonary vascular diseases (PVD). PVDs are characterized by exertional dyspnoea and oxygen desaturation, and reduced quality of life and survival. Medical therapies improve life expectancy and physical performance of PVD patients, of whom many wish to participate in professional work and recreational activities including traveling to high altitude. The exposure to the hypobaric hypoxic environment of mountain regions incurs the risk of high altitude adverse events (AEHA) due to severe hypoxaemia exacerbating symptoms and further increase in pulmonary artery pressure, which may lead to right heart decompensation. Recent prospective and randomized trials show that altitude‐induced hypoxaemia, pulmonary haemodynamic changes and impairment of exercise performance in PVD patients are in the range found in healthy people. The vast majority of optimally treated stable PVD patients who do not require long‐term oxygen therapy at low altitude can tolerate short‐term exposure to moderate altitudes up to 2500 m. PVD patients that reveal persistent severe resting hypoxaemia ( <80% for >30 min) at 2500 m respond well to supplemental oxygen therapy. Although there are no accurate predictors for AEHA, PVD patients with unfavourable risk profiles at low altitude, such as higher WHO functional class, lower exercise capacity with more pronounced exercise‐induced desaturation and more severely impaired haemodynamics, are at increased risk of AEHA. Therefore, doctors with experience in PVD and high‐altitude medicine should counsel PVD patients before any high‐altitude sojourn. This review aims to summarize recent literature and clinical recommendations about PVD patients travelling to high altitude.
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Relegated to the Sidelines: A Qualitative Inquiry of Gatekeepers’ Perspectives and Values of Physical Education for Disabled Children
Authors: McNamara Scott W.T. , Craig Patrica , Henly Megan , Gravink Jill 
Published: May 2024

Several institutional aspects within the U.S. public school system impede the delivery of adapted physical education (APE) services to disabled children, including a lack of understanding and prioritization of these services by the special education team and a lack of qualified APE professionals to deliver these services. Thus, we conducted a qualitative inquiry grounded in a critical-ableism perspective to explore special education gatekeepers’ experiences and perspectives of APE. Gatekeepers included parents, physical educators, and school administrators. Using a reflexive thematic analysis, we developed four interrelated themes: (a) disregard, negative, and charity mindsets toward disability; (b) systemic challenges in valuing and prioritizing APE; (c) presence as inclusion: (un)intentional marginalization in physical education; and (d) physical education for my child was a nightmare. These findings illustrate the complexities around the provision of physical education and APE to disabled children.
Core genome multilocus sequence typing (cgMLST) applicable to the monophyletic
Klebsiella oxytoca
species complex
Authors: Dabernig-Heinz Johanna , Wagner Gabriel E. , Prior Karola , Lipp Michaela , Kienesberger Sabine , Ruppitsch Werner , Rønning Torunn G. , Harmsen Dag , Steinmetz Ivo , Leitner Eva 
Published: May 2024

ABSTRACT

The environmental bacterium
Klebsiella oxytoca
displays an alarming increase of antibiotic-resistant strains that frequently cause outbreaks in intensive care units. Due to its prevalence in the environment and opportunistic presence in humans, molecular surveillance (including resistance marker screening) and high-resolution cluster analysis are of high relevance. Furthermore,
K. oxytoca
previously described in studies is rather a species complex (KoSC) than a single species comprising at least six closely related species that are not easily differentiated by standard typing methods. To reach a discriminatory power high enough to identify and resolve clusters within these species, whole genome sequencing is necessary. The resolution is achievable with core genome multilocus sequence typing (cgMLST) extending typing of a few housekeeping genes to thousands of core genome genes. CgMLST is highly standardized and provides a nomenclature enabling cross laboratory reproducibility and data exchange for routine diagnostics. Here, we established a cgMLST scheme not only capable of resolving the KoSC species but also producing reliable and consistent results for published outbreaks. Our cgMLST scheme consists of 2,536 core genome and 2,693 accessory genome targets, with a percentage of good cgMLST targets of 98.31% in 880 KoSC genomes downloaded from the National Center for Biotechnology Information (NCBI). We also validated resistance markers against known resistance gene patterns and successfully linked genetic results to phenotypically confirmed toxic strains carrying the
til
gene cluster. In conclusion, our novel cgMLST enables highly reproducible typing of four different clinically relevant species of the KoSC and thus facilitates molecular surveillance and cluster investigations.
Indiscriminate activities of different henipavirus polymerase complex proteins allow for efficient minigenome replication in hybrid systems
Authors: Li Xiao , Yang Yanling , López Carolina B. 
Published: May 2024

ABSTRACT

The henipaviruses, including Nipah virus (NiV) and Hendra virus (HeV), are biosafety level 4 (BSL-4) zoonotic pathogens that cause severe neurological and respiratory disease in humans. To study the replication machinery of these viruses, we developed robust minigenome systems that can be safely used in BSL-2 conditions. The nucleocapsid (N), phosphoprotein (P), and large protein (L) of henipaviruses are critical elements of their replication machinery and thus essential support components of the minigenome systems. Here, we tested the effects of diverse combinations of the replication support proteins on the replication capacity of the NiV and HeV minigenomes by exchanging the helper plasmids coding for these proteins among the two viruses. We demonstrate that all combinations including one or more heterologous proteins were capable of replicating both the NiV and HeV minigenomes. Sequence alignment showed identities of 92% for the N protein, 67% for P, and 87% for L. Notably, variations in amino acid residues were not concentrated in the N-P and P-L interacting regions implying that dissimilarities in amino acid composition among NiV and HeV polymerase complex proteins may not impact their interactions. The observed indiscriminate activity of NiV and HeV polymerase complex proteins is different from related viruses, which can support the replication of heterologous genomes only when the whole polymerase complex belongs to the same virus. This newly observed promiscuous property of the henipavirus polymerase complex proteins likely attributed to their conserved interaction regions could potentially be harnessed to develop universal anti-henipavirus antivirals.

IMPORTANCE
Given the severity of disease induced by Hendra and Nipah viruses in humans and the continuous emergence of new henipaviruses as well as henipa-like viruses, it is necessary to conduct a more comprehensive investigation of the biology of henipaviruses and their interaction with the host. The replication of henipaviruses and the development of antiviral agents can be studied in systems that allow experiments to be performed under biosafety level 2 conditions. Here, we developed robust minigenome systems for the Nipah virus (NiV) and Hendra virus (HeV) that provide a convenient alternative for studying NiV and HeV replication. Using these systems, we demonstrate that any combination of the three polymerase complex proteins of NiV and HeV could effectively initiate the replication of both viral minigenomes, which suggests that the interaction regions of the polymerase complex proteins could be effective targets for universal and effective anti-henipavirus interventions.
Coding-complete genome sequence and phylogenetic analysis of Coxsackievirus B3 isolated from South Korea
Authors: Ga Yun Ji , Go Yun Young , Yeh Jung-Yong 
Published: May 2024

ABSTRACT

Whole-genome sequencing of a Coxsackievirus B3 strain isolated from the stool of a febrile patient with aseptic meningoencephalitis, South Korea, in 2002 was performed. This strain exhibits a high nucleotide sequence identity with various strains circulating in China from 2001 to 2019.
Early Mississippian global δ13C excursion is not a diagenetic artifact
Authors: Braun Matthew G. , Anderson Noah T. , Bergmann Kristin D. , Griffith Elizabeth M. , Saltzman Matthew R. 
Published: May 2024

Abstract
Shallow-water platform carbonate δ13C may provide a record of changes in ocean chemistry through time, but early marine diagenesis and local processes can decouple these records from the global carbon cycle. Recent studies of calcium isotopes (δ44/40Ca) in shallow-water carbonates indicate that δ44/40Ca can be altered during early marine diagenesis, implying that δ13C may also potentially be altered. Here, we tested the hypothesis that the platform carbonate δ13C record of the Kinderhookian–Osagean boundary excursion (KOBE), ~353 m.y. ago, reflects a period of global diagenesis using paired isotopic (δ44/40Ca and clumped isotopes) and trace-element geochemistry from three sections in the United States. There is little evidence for covariation between δ44/40Ca and δ13C during the KOBE. Clumped isotopes from our shallowest section support primarily sediment-buffered diagenesis at relatively low temperatures. We conclude that the δ13C record of the KOBE as recorded in shallow-water carbonate is consistent with a shift in the dissolved inorganic carbon reservoir and that, more generally, ancient shallow-water carbonates can retain records of primary seawater chemistry.
Association between exposure to antibiotics during pregnancy or early infancy and risk of autism spectrum disorder, intellectual disorder, language disorder, and epilepsy in children: population based cohort study
Authors: Choi Ahhyung , Lee Hyesung , Jeong Han Eol , Lee Seo-Young , Kwon Jun Soo , Han Jung Yeol , Choe Young June , Shin Ju-Young 
Published: May 2024

Abstract

Objective
To evaluate the association between antibiotic use during pregnancy or early infancy and the risk of neurodevelopmental disorders in children.

Design
Nationwide population based cohort study and sibling analysis.

Setting
Korea’s National Health Insurance Service mother-child linked database, 2008-21.

Participants
All children live born between 2009 and 2020, followed up until 2021 to compare those with and without antibiotic exposure during pregnancy or early infancy (first six months of life).

Main outcomes measures
Autism spectrum disorder, intellectual disorder, language disorder, and epilepsy in children. After 1:1 propensity score matching based on many potential confounders, hazard ratios with 95% confidence interval were estimated using Cox proportional hazard models. A sibling analysis additionally accounted for unmeasured familial factors.

Results
After propensity score matching, 1 961 744 children were identified for the pregnancy analysis and 1 609 774 children were identified for the early infancy analysis. Although antibiotic exposure during pregnancy was associated with increased risks of all four neurodevelopmental disorders in the overall cohort, these estimates were attenuated towards the null in the sibling analyses (hazard ratio for autism spectrum disorder 1.06, 95% confidence interval 1.01 to 1.12; intellectual disorder 1.00, 0.93 to 1.07; language disorder 1.05, 1.02 to 1.09; and epilepsy 1.03, 0.98 to 1.08). Likewise, no association was observed between antibiotic exposure during early infancy and autism spectrum disorder (hazard ratio 1.00, 0.96 to 1.03), intellectual disorder (1.07, 0.98 to 1.15), and language disorder (1.04, 1.00 to 1.08) in the sibling analyses; however, a small increased risk of epilepsy was observed (1.13, 1.09 to 1.18). The results generally remained consistent across several subgroup and sensitivity analyses, except for slightly elevated risks observed among children who used antibiotics during very early life and those who used antibiotics for more than 15 days.

Conclusions
In this large cohort study, antibiotic exposure during pregnancy or early infancy was not associated with an increased risk of autism spectrum disorder, intellectual disorder, or language disorder in children. However, elevated risks were observed in several subgroups such as children using antibiotics during very early life and those with long term antibiotic use, which warrants attention and further investigation. Moreover, antibiotic use during infancy was modestly associated with epilepsy, even after control for indications and familial factors. When prescribing antibiotics to pregnant women and infants, clinicians should carefully balance the benefits of use against potential risks.
PET imaging of unruptured intracranial aneurysm inflammation (PET-IA) study: a feasibility study protocol
Authors: Laukka Dan , Paturi Jooa , Rahi Melissa , Saraste Antti , Parkkola Riitta , Kivelev Juri , Gardberg Maria , Kuhmonen Johanna , Rinne Jaakko 
Published: May 2024

IntroductionPositron emission tomography (PET) imaging can be used to evaluate arterial wall inflammation in extracranial vascular diseases. However, the application of PET imaging in unruptured intracranial aneurysms (UIA) remains unexplored. Our objective is to investigate feasibility of PET imaging using 18F-FDG and 68Ga-DOTANOC tracers to evaluate arterial wall inflammation in UIA.Methods and analysisThis PET imaging feasibility study will enrol patients scheduled for surgical treatment of UIA. The study subjects will undergo PET imaging of the intracranial arteries within 1 month before planned surgery. The imaging protocol includes 18F-FDG PET MRI, MRA with gadolinium enhancement, and 68Ga-DOTANOC PET CT. The study will also involve preoperative blood samples, intraoperative cerebrospinal fluid (CSF) samples, and aneurysm sac biopsy. Planned sample size is at least 18 patients. Primary outcome is uptake of 18F-FDG or 68Ga-DOTANOC in intracranial arterial aneurysms compared with contralateral normal vessel as maximum standardised uptake value or target-to-blood pool ratio and correlation of uptake of 18F-FDG or 68Ga-DOTANOC to aneurysm histological findings. Secondary outcomes include estimating the correlations between uptake of 18F-FDG or 68Ga-DOTANOC and histological findings with blood and CSF miRNA-levels, arterial wall enhancement in gadolinium enhanced MRA, aneurysm size and shape, smoking, hypertension, and location of the aneurysm.Ethics and disseminationThis study is approved by the Human Research Ethics Committee of the Hospital District of Southwest Finland, Finnish Medicines Agency Fimea, and Turku University Hospital. Findings will be disseminated through peer-reviewed journal articles and presentations at national and international conferences.Trial registration numberNCT04715503
Cross-sectional analysis of use of real-world data in single technology appraisals of oncological medicine by the National Institute for Health and Care Excellence in 2011–2021
Authors: Kang Jiyeon , Cairns John 
Published: May 2024

ObjectivesThis study aims to identify how real-world data (RWD) have been used in single technology appraisals (STAs) of cancer drugs by the National Institute for Health and Care Excellence (NICE).DesignCross-sectional study of NICE technology appraisals of cancer drugs for which guidance was issued between January 2011 and December 2021 (n=229). The appraisals were reviewed following a published protocol to extract the data about the use of RWD. The use of RWD was analysed by reviewing the specific ways in which RWD were used and by identifying different patterns of use.Primary outcome measureThe number of appraisals where RWD are used in the economic modelling.ResultsMost appraisals used RWD in their economic models. The parametric use of RWD was commonly made in the economic models (76% of the included appraisals), whereas non-parametric use was less common (41%). Despite widespread use of RWD, there was no dominant pattern of use. Three sources of RWD (registries, administrative data, chart reviews) were found across the three important parts of the economic model (choice of comparators, overall survival and volume of treatment).ConclusionsNICE has had a long-standing interest in the use of RWD in STAs. A systematic review of oncology appraisals suggests that RWD have been widely used in diverse parts of the economic models. Between 2011 and 2021, parametric use was more commonly found in economic models than non-parametric use. Nonetheless, there was no clear pattern in the way these data were used. As each appraisal involves a different decision problem and the ability of RWD to provide the information required for the economic modelling varies, appraisals will continue to differ with respect to their use of RWD.
Work environment and hypertension in industrial settings in Benin in 2019: a cross-sectional study
Authors: Adjobimey Menonli , Houehanou Corine Yessito , Cisse Ibrahim Mama , Mikponhoue Rose , Hountohotegbe Esdras , Tchibozo Concheta , Adjogou Amelée , Dossougbété Véronique , Gounongbe Fabien , Ayelo Paul Ahoumenou , Hinson Vikkey , Houinato Dismand Stephan 
Published: May 2024

ObjectiveTo determine the association between occupational factors, particularly psychosocial factors, and hypertension.DesignDescriptive and analytical cross-sectional study using logistic multivariate regression.SettingFifteen cotton ginning plants in Benin.ParticipantsPermanent and occasional workers in the cotton ginning industry.Data collectionData on sociodemographic, occupational, behavioural and clinical history characteristics were collected using a number of standardised, interviewer-administered questionnaires. These questionnaires were based on the WHO’s non-communicable disease questionnaire, Karasek questionnaire and Siegrist questionnaire. Weight, height and blood pressure were measured. Any worker with systolic blood pressure ≥140 mm Hg and/or diastolic blood pressure ≥90 mm Hg according to the WHO criteria was considered hypertensive, as was any subject on antihypertensive treatment even if blood pressure was normal.ResultsA total of 1883 workers were included, with a male to female ratio of 9.08. Of these, 510 suffered from hypertension (27.1%, 95% CI 25.1 to 29.2). In the multivariate analysis, the risk factors identified were occupational stress (adjusted OR (aOR)=3.96, 95% CI 1.28 to 12.2), age ≥25 years (aOR=2.77, 95% CI 1.55 to 4.96), body mass index of 25–30 kg/m2(aOR=1.71, 95% CI 1.32 to 2.2), body mass index >30 kg/m2(aOR=2.74, 95% CI 1.84 to 4.09), permanent worker status (aOR=1.66, 95% CI 1.44 to 2.41) and seniority in the textile sector >5 years (aOR=2.18, 95% CI 1.7 to 2.8). Recognition at work emerged as an effect-modifying factor subject to stratification.ConclusionsOccupational factors, particularly job strain and recognition at work, are modifiable factors associated with hypertension in the ginning plants sector and deserve to be corrected through occupational health promotion and prevention.