bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a micro-combined heat and power powered by an opposed-piston engine in building applications
Authors: Gao Zhiming , Zoldak Philip , Beaudry-Losique Jacques , Mannarino Tony , Mansinger Jonathan , Molana Maysam , Zhang Mingkan , Cheekatamarla Praveen , Abuheiba Ahmed , Li Hailin , Fricke Brian , Nawaz Kashif 
Published: May 2024

AbstractResidential homes and light commercial buildings usually require substantial heat and electricity simultaneously. A combined heat and power system enables more efficient and environmentally friendly energy usage than that achieved when heat and electricity are produced in separate processes. However, due to financial and space constraints, residential and light commercial buildings often limit the use of traditional large-scale industrial equipment. Here we develop a micro–combined heat and power system powered by an opposed-piston engine to simultaneously generate electricity and provide heat to residential homes or light commercial buildings. The developed prototype attains the maximum AC electrical efficiency of 35.2%. The electrical efficiency breaks the typical upper boundary of 30% for micro–combined heat and power systems using small internal combustion engines (i.e., <10 kW). Moreover, the developed prototype enables maximum combined electrical and thermal efficiencies greater than 93%. The prototype is optimally designed for natural gas but can also run renewable biogas and hydrogen, supporting the transition from current conventional fossil fuels to zero carbon emissions in the future. The analysis of the unit’s decarbonization and cost-saving potential indicate that, except for specific locations, the developed prototype might excel in achieving decarbonization and cost savings primarily in US northern and middle climate zones.
Stable pure-green organic light-emitting diodes toward Rec.2020 standard
Authors: Tang Xun , Tsagaantsooj Tuul , Rajakaruna Tharindu P. B. , Wang Kai , Chen Xian-Kai , Zhang Xiao-Hong , Hatakeyama Takuji , Adachi Chihaya 
Published: May 2024

AbstractManipulating dynamic behaviours of charge carriers and excitons in organic light-emitting diodes (OLEDs) is essential to simultaneously achieve high colour purity and superior operational lifetime. In this work, a comprehensive transient electroluminescence investigation reveals that incorporating a thermally activated delayed fluorescence assistant molecule with a deep lowest unoccupied molecular orbital into a bipolar host matrix effectively traps the injected electrons. Meanwhile, the behaviours of hole injection and transport are still dominantly governed by host molecules. Thus, the recombination zone notably shifts toward the interface between the emissive layer (EML) and the electron-transporting layer (ETL). To mitigate the interfacial carrier accumulation and exciton quenching, this bipolar host matrix could serve as a non-barrier functional spacer between EML/ETL, enabling the distribution of recombination zone away from this interface. Consequently, the optimized OLED exhibits a low driving voltage, promising device stability (95% of the initial luminance of 1000 cd m−2, LT95 > 430 h), and a high Commission Internationale de L’Éclairage y coordinate of 0.69. This indicates that managing the excitons through rational energy level alignment holds the potential for simultaneously satisfying Rec.2020 standard and achieving commercial-level stability.
A rationally designed miniature of soluble methane monooxygenase enables rapid and high-yield methanol production in Escherichia coli
Authors: Yu Yeonhwa , Shi Yongfan , Kwon Young Wan , Choi Yoobin , Kim Yusik , Na Jeong-Geol , Huh June , Lee Jeewon 
Published: May 2024

AbstractSoluble methane monooxygenase (sMMO) oxidizes a wide range of carbon feedstocks (C1 to C8) directly using intracellular NADH and is a useful means in developing green routes for industrial manufacturing of chemicals. However, the high-throughput biosynthesis of active recombinant sMMO and the ensuing catalytic oxidation have so far been unsuccessful due to the structural and functional complexity of sMMO, comprised of three functionally complementary components, which remains a major challenge for its industrial applications. Here we develop a catalytically active miniature of sMMO (mini-sMMO), with a turnover frequency of 0.32 s−1, through an optimal reassembly of minimal and modified components of sMMO on catalytically inert and stable apoferritin scaffold. We characterise the molecular characteristics in detail through in silico and experimental analyses and verifications. Notably, in-situ methanol production in a high-cell-density culture of mini-sMMO-expressing recombinant Escherichia coli resulted in higher yield and productivity (~ 3.0 g/L and 0.11 g/L/h, respectively) compared to traditional methanotrophic production.
Translation initiation factor eIF1.2 promotes Toxoplasma stage conversion by regulating levels of key differentiation factors
Authors: Wang Fengrong , Holmes Michael J. , Hong Hea Jin , Thaprawat Pariyamon , Kannan Geetha , Huynh My-Hang , Schultz Tracey L. , Licon M. Haley , Lourido Sebastian , Dong Wenzhao , Brito Querido Jailson , Sullivan William J. , O’Leary Seán E. , Carruthers Vern B. 
Published: May 2024

AbstractThe parasite Toxoplasma gondii persists in its hosts by converting from replicating tachyzoites to latent bradyzoites housed in tissue cysts. The molecular mechanisms that mediate T. gondii differentiation remain poorly understood. Through a mutagenesis screen, we identified translation initiation factor eIF1.2 as a critical factor for T. gondii differentiation. A F97L mutation in eIF1.2 or the genetic ablation of eIF1.2 (∆eif1.2) markedly impeded bradyzoite cyst formation in vitro and in vivo. We demonstrated, at single-molecule level, that the eIF1.2 F97L mutation impacts the scanning process of the ribosome preinitiation complex on a model mRNA. RNA sequencing and ribosome profiling experiments unveiled that ∆eif1.2 parasites are defective in upregulating bradyzoite induction factors BFD1 and BFD2 during stress-induced differentiation. Forced expression of BFD1 or BFD2 significantly restored differentiation in ∆eif1.2 parasites. Together, our findings suggest that eIF1.2 functions by regulating the translation of key differentiation factors necessary to establish chronic toxoplasmosis.
Link between supercurrent diode and anomalous Josephson effect revealed by gate-controlled interferometry
Authors: Reinhardt S. , Ascherl T. , Costa A. , Berger J. , Gronin S. , Gardner G. C. , Lindemann T. , Manfra M. J. , Fabian J. , Kochan D. , Strunk C. , Paradiso N. 
Published: May 2024

AbstractIn Josephson diodes the asymmetry between positive and negative current branch of the current-phase relation leads to a polarity-dependent critical current and Josephson inductance. The supercurrent nonreciprocity can be described as a consequence of the anomalous Josephson effect —a φ0-shift of the current-phase relation— in multichannel ballistic junctions with strong spin-orbit interaction. In this work, we simultaneously investigate φ0-shift and supercurrent diode efficiency on the same Josephson junction by means of a superconducting quantum interferometer. By electrostatic gating, we reveal a direct link between φ0-shift and diode effect. Our findings show that spin-orbit interaction in combination with a Zeeman field plays an important role in determining the magnetochiral anisotropy and the supercurrent diode effect.
ZO-1 interacts with YB-1 in endothelial cells to regulate stress granule formation during angiogenesis
Authors: El Bakkouri Yassine , Chidiac Rony , Delisle Chantal , Corriveau Jeanne , Cagnone Gael , Gaonac’h-Lovejoy Vanda , Chin Ashley , Lécuyer Éric , Angers Stephane , Joyal Jean-Sébastien , Topisirovic Ivan , Hulea Laura , Dubrac Alexandre , Gratton Jean-Philippe 
Published: May 2024

AbstractZonula occludens-1 (ZO-1) is involved in the regulation of cell-cell junctions between endothelial cells (ECs). Here we identify the ZO-1 protein interactome and uncover ZO-1 interactions with RNA-binding proteins that are part of stress granules (SGs). Downregulation of ZO-1 increased SG formation in response to stress and protected ECs from cellular insults. The ZO-1 interactome uncovered an association between ZO-1 and Y-box binding protein 1 (YB-1), a constituent of SGs. Arsenite treatment of ECs decreased the interaction between ZO-1 and YB-1, and drove SG assembly. YB-1 expression is essential for SG formation and for the cytoprotective effects induced by ZO-1 downregulation. In the developing retinal vascular plexus of newborn mice, ECs at the front of growing vessels express less ZO-1 but display more YB-1-positive granules than ECs located in the vascular plexus. Endothelial-specific deletion of ZO-1 in mice at post-natal day 7 markedly increased the presence of YB-1-positive granules in ECs of retinal blood vessels, altered tip EC morphology and vascular patterning, resulting in aberrant endothelial proliferation, and arrest in the expansion of the retinal vasculature. Our findings suggest that, through its interaction with YB-1, ZO-1 controls SG formation and the response of ECs to stress during angiogenesis.
Impact of secretin receptor homo-dimerization on natural ligand binding
Authors: Harikumar Kaleeckal G. , Piper Sarah J. , Christopoulos Arthur , Wootten Denise , Sexton Patrick M. , Miller Laurence J. 
Published: May 2024

AbstractClass B G protein-coupled receptors can form dimeric complexes important for high potency biological effects. Here, we apply pharmacological, biochemical, and biophysical techniques to cells and membranes expressing the prototypic secretin receptor (SecR) to gain insights into secretin binding to homo-dimeric and monomeric SecR. Spatial proximity between peptide and receptor residues, probed by disulfide bond formation, demonstrates that the secretin N-terminus moves from adjacent to extracellular loop 3 (ECL3) at wild type SecR toward ECL2 in non-dimerizing mutants. Analysis of fluorescent secretin analogs demonstrates stable engagement of the secretin C-terminal region within the receptor extracellular domain (ECD) for both dimeric and monomeric receptors, while the mid-region exhibits lower mobility while docked at the monomer. Moreover, decoupling of G protein interaction reduces mobility of the peptide mid-region at wild type receptor to levels similar to the mutant, whereas it has no further impact on the monomer. These data support a model of peptide engagement whereby the ability of SecR to dimerize promotes higher conformational dynamics of the peptide-bound receptor ECD and ECLs that likely facilitates more efficient G protein recruitment and activation, consistent with the higher observed functional potency of secretin at wild type SecR relative to the monomeric mutant receptor.
A comparative study of different antiviral treatment protocols in HCV related cryoglobulinemic vasculitis
Authors: Allam Walaa Ramadan , Hegazy Mohamed Tharwat , Hussein Mohamed A. , Zoheir Naguib , Quartuccio Luca , El-Khamisy Sherif F. , Ragab Gaafar 
Published: May 2024

AbstractThe treatment of HCV and its sequelae are used to be predominantly based on Interferon (IFN). However, this was associated with significant adverse events as a result of its immunostimulant capabilities. Since their introduction, the directly acting antiviral drugs (DAAs), have become the standard of care to treat of HCV and its complications including mixed cryoglobulinemic vasculitis (MCV). In spite of achieving sustained viral response (SVR), there appeared many reports describing unwelcome complications such as hepatocellular and hematological malignancies as well as relapses. Prolonged inflammation induced by a multitude of factors, can lead to DNA damage and affects BAFF and APRIL, which serve as markers of B-cell proliferation. We compared, head-to-head, three antiviral protocols for HCV–MCV treatment As regards the treatment response and relapse, levels of BAFF and APRIL among pegylated interferon α-based and free regimens (Sofosbuvir + Ribavirin; SOF–RIBA, Sofosbuvir + Daclatasvir; SOF–DACLA). Regarding clinical response HCV–MCV and SVR; no significant differences could be identified among the 3 different treatment protocols, and this was also independent form using IFN. We found no significant differences between IFN-based and free regimens DNA damage, markers of DNA repair, or levels of BAFF and APRIL. However, individualized drug-to-drug comparisons showed many differences. Those who were treated with IFN-based protocol showed decreased levels of DNA damage, while the other two IFN-free groups showed increased DNA damage, being the worst in SOF–DACLA group. There were increased levels of BAFF through follow-up periods in the 3 protocols being the best in SOF–DACLA group (decreased at 24 weeks). In SOF–RIBA, CGs relapsed significantly during the follow-up period. None of our patients who were treated with IFN-based protocol had significant clinico-laboratory relapse. Those who received IFN-free DAAs showed a statistically significant relapse of constitutional manifestations. Our findings suggest that IFN-based protocols are effective in treating HCV–MCV similar to IFN-free protocols. They showed lower levels of DNA damage and repair. We believe that our findings may offer an explanation for the process of lymphoproliferation, occurrence of malignancies, and relapses by shedding light on such possible mechanisms.
Characterizing the human intestinal chondroitin sulfate glycosaminoglycan sulfation signature in inflammatory bowel disease
Authors: Francis Kendra L. , Zheng Hengqi B. , Suskind David L. , Murphree Taylor A. , Phan Bao Anh , Quah Emily , Hendrickson Aarun S. , Zhou Xisheng , Nuding Mason , Hudson Alexandra S. , Guttman Miklos , Morton Gregory J. , Schwartz Michael W. , Alonge Kimberly M. , Scarlett Jarrad M. 
Published: May 2024

AbstractThe intestinal extracellular matrix (ECM) helps maintain appropriate tissue barrier function and regulate host-microbial interactions. Chondroitin sulfate- and dermatan sulfate-glycosaminoglycans (CS/DS-GAGs) are integral components of the intestinal ECM, and alterations in CS/DS-GAGs have been shown to significantly influence biological functions. Although pathologic ECM remodeling is implicated in inflammatory bowel disease (IBD), it is unknown whether changes in the intestinal CS/DS-GAG composition are also linked to IBD in humans. Our aim was to characterize changes in the intestinal ECM CS/DS-GAG composition in intestinal biopsy samples from patients with IBD using mass spectrometry. We characterized intestinal CS/DS-GAGs in 69 pediatric and young adult patients (n = 13 control, n = 32 active IBD, n = 24 IBD in remission) and 6 adult patients. Here, we report that patients with active IBD exhibit a significant decrease in the relative abundance of CS/DS isomers associated with matrix stability (CS-A and DS) compared to controls, while isomers implicated in matrix instability and inflammation (CS-C and CS-E) were significantly increased. This imbalance of intestinal CS/DS isomers was restored among patients in clinical remission. Moreover, the abundance of pro-stabilizing CS/DS isomers negatively correlated with clinical disease activity scores, whereas both pro-inflammatory CS-C and CS-E content positively correlated with disease activity scores. Thus, pediatric patients with active IBD exhibited increased pro-inflammatory and decreased pro-stabilizing CS/DS isomer composition, and future studies are needed to determine whether changes in the CS/DS-GAG composition play a pathogenic role in IBD.
Softly empowering a prosocial expert in the family: lasting effects of a counter-misinformation intervention in an informational autocracy
Authors: Orosz Gábor , Faragó Laura , Paskuj Benedek , Rakovics Zsófia , Sam-Mine Diane , Audemard Gilles , Modeliar Mouny Samy , Krekó Péter 
Published: May 2024

AbstractThe present work is the first to comprehensively analyze the gravity of the misinformation problem in Hungary, where misinformation appears regularly in the pro-governmental, populist, and socially conservative mainstream media. In line with international data, using a Hungarian representative sample (Study 1, N = 991), we found that voters of the reigning populist, conservative party could hardly distinguish fake from real news. In Study 2, we demonstrated that a prosocial intervention of ~ 10 min (N = 801) helped young adult participants discern misinformation four weeks later compared to the control group without implementing any boosters. This effect was the most salient regarding pro-governmental conservative fake news content, leaving real news evaluations intact. Although the hypotheses of the present work were not preregistered, it appears that prosocial misinformation interventions might be promising attempts to counter misinformation in an informational autocracy in which the media is highly centralized. Despite using social motivations, it does not mean that long-term cognitive changes cannot occur. Future studies might explore exactly how these interventions can have an impact on the long-term cognitive processing of news content as well as their underlying neural structures.
Differences in childhood stress between Neanderthals and early modern humans as reflected by dental enamel growth disruptions
Authors: Limmer Laura Sophia , Santon Matteo , McGrath Kate , Harvati Katerina , El Zaatari Sireen 
Published: May 2024

AbstractNeanderthals’ lives were historically portrayed as highly stressful, shaped by constant pressures to survive in harsh ecological conditions, thus potentially contributing to their extinction. Recent work has challenged this interpretation, leaving the issue of stress among Paleolithic populations highly contested and warranting in-depth examination. Here, we analyze the frequency of dental enamel hypoplasia, a growth disruption indicator of early life stress, in the largest sample of Neanderthal and Upper Paleolithic dentitions investigated to date for these features. To track potential species-specific patterns in the ontogenetic distribution of childhood stress, we present the first comprehensive Bayesian modelling of the likelihood of occurrence of individual and matched enamel growth disruptions throughout ontogeny. Our findings support similar overall stress levels in both groups but reveal species-specific patterns in its ontogenetic distribution. While Neanderthal children faced increasing likelihoods of growth disruptions starting with the weaning process and culminating in intensity post-weaning, growth disruptions in Upper Paleolithic children were found to be limited around the period of weaning and substantially dropping after its expected completion. These results might, at least in part, reflect differences in childcare or other behavioral strategies between the two taxa, including those that were advantageous for modern humans’ long-term survival.
Long-COVID autonomic syndrome in working age and work ability impairment
Authors: Rinaldi Luca , Rigo Stefano , Pani Margherita , Bisoglio Andrea , Khalaf Kareem , Minonzio Maura , Shiffer Dana , Romeo Maria Angela , Verzeletti Paolo , Ciccarelli Michele , Bordoni Maria Grazia , Stranges Saverio , Riboli Elio , Furlan Raffaello , Barbic Franca 
Published: May 2024

AbstractLong-COVID19 has been recently associated with long-sick leave and unemployment. The autonomic nervous system functioning may be also affected by SARS-CoV-2, leading to a chronic autonomic syndrome. This latter remains widely unrecognized in clinical practice. In the present study, we assessed the occurrence of Long-COVID19 Autonomic Syndrome in a group of active workers as well as the relationships between their autonomic dysfunction and work ability. This prospective observational study was conducted during the 2nd wave of the pandemic in Italy. Forty-five patients (53.6 ± 8.4 years; 32 M) hospitalized for COVID19, were consecutively enrolled at the time of their hospital discharge (T0) and followed-up for 6 months. Autonomic symptoms and work ability were assessed by COMPASS31 and Work Ability Index questionnaires at T0, one (T1), three and six (T6) months after hospital discharge and compared to those retrospectively collected for a period preceding SARS-CoV-2 infection. Clinical examination and standing test were also performed at T1 and T6. One in three working-age people developed a new autonomic syndrome that was still evident 6 months after the acute infection resolution. This was associated with a significant reduction in the work ability. Recognition of Long-COVID19 Autonomic Syndrome may promote early intervention to facilitate return to work and prevent unemployment.
Enhancing trash classification in smart cities using federated deep learning
Authors: Ahmed Khan Haroon , Naqvi Syed Saud , Alharbi Abeer A. K. , Alotaibi Salihah , Alkhathami Mohammed 
Published: May 2024

AbstractEfficient Waste management plays a crucial role to ensure clean and green environment in the smart cities. This study investigates the critical role of efficient trash classification in achieving sustainable solid waste management within smart city environments. We conduct a comparative analysis of various trash classification methods utilizing deep learning models built on convolutional neural networks (CNNs). Leveraging the PyTorch open-source framework and the TrashBox dataset, we perform experiments involving ten unique deep neural network models. Our approach aims to maximize training accuracy. Through extensive experimentation, we observe the consistent superiority of the ResNext-101 model compared to others, achieving exceptional training, validation, and test accuracies. These findings illuminate the potential of CNN-based techniques in significantly advancing trash classification for optimized solid waste management within smart city initiatives. Lastly, this study presents a distributed framework based on federated learning that can be used to optimize the performance of a combination of CNN models for trash detection.
Repetitive transcranial magnetic stimulation focusing on patients with neuropathic pain in the upper limb: a randomized sham-controlled parallel trial
Authors: Mori Nobuhiko , Hosomi Koichi , Nishi Asaya , Miyake Akimitsu , Yamada Tomomi , Matsugi Akiyoshi , Jono Yasutomo , Lim Chanseok , Khoo Hui Ming , Tani Naoki , Oshino Satoru , Saitoh Youichi , Kishima Haruhiko 
Published: May 2024

AbstractThis study aimed to evaluate the efficacy and safety of navigation-guided repetitive transcranial magnetic stimulation (rTMS) over the primary motor cortex in patients with neuropathic pain in the upper limb. This randomized, blinded, sham-controlled, parallel trial included a rTMS protocol (10-Hz, 2000 pulses/session) consisting of five daily sessions, followed by one session per week for the next seven weeks. Pain intensity, as well as pain-related disability, quality of life, and psychological status, were assessed. For the primary outcome, pain intensity was measured daily using a numerical rating scale as a pain diary. Thirty patients were randomly assigned to the active rTMS or sham-stimulation groups. In the primary outcome, the decrease (least square [LS] mean ± standard error) in the weekly average of a pain diary at week 9 compared to the baseline was 0.84 ± 0.31 in the active rTMS group and 0.58 ± 0.29 in the sham group (LS mean difference, 0.26; 95% confidence interval, − 0.60 to 1.13). There was no significant effect on the interaction between the treatment group and time point. Pain-related disability score improved, but other assessments showed no differences. No serious adverse events were observed. This study did not show significant pain relief; however, active rTMS tended to provide better results than sham. rTMS has the potential to improve pain-related disability in addition to pain relief.Clinical Trial Registration number: jRCTs052190110 (20/02/2020).