bluuebunny/crossref_metadata_2025_split huggingface dataset:
A combination of chlorzoxazone and folic acid improves recognition memory, anxiety and depression in SCA3-84Q mice
Authors: Marinina Ksenia S , Bezprozvanny Ilya B , Egorova Polina A 
Published: May 2024

Abstract
Spinocerebellar ataxia type 3 (SCA3), also known as Machado-Joseph disease, is reported to be the most common type of autosomal dominant cerebellar ataxia (ADCA). SCA3 patients suffer from a progressive decline in motor coordination and other disease-associated symptoms. Moreover, recent studies have reported that SCA3 patients also exhibit symptoms of cerebellar cognitive affective syndrome (CCAS). We previously observed signs of CCAS in mouse model of SCA3. Particularly, SCA3-84Q mice suffer from anxiety, recognition memory decline, and also exhibit signs of low mood and aversion to activity. Here we studied the effect of long-term injections of SK channels activator chlorzoxazone (CHZ) together and separately with the folic acid (FA) on the cerebellar Purkinje cell (PC) firing and histology, and also on the motor and cognitive functions as well as mood alterations in SCA3-84Q hemizygous transgenic mice. We realized that both CHZ and CHZ-FA combination had similar positive effect on pure cerebellum impairments including PC firing precision, PC histology, and motor performance in SCA3-84Q mice. However, only the CHZ-FA combination, but not CHZ, had significantly ameliorated the signs of anxiety and depression, and also noticeably improved recognition memory in SCA3-84Q mice. Our results suggest that the combination therapy for both ataxia and non-motor symptoms is required for the complex treatment of ADCA.
Contributing to ecosystem-based management: a personal scientific journey
Authors: Trenkel Verena M 
Published: May 2024

Abstract
After three decades of working as a research scientist, I am stepping back to consider the events, questions, and principles that have guided my scientific journey. Important questions and research objectives have been how to implement the ecosystem approach to fisheries management in practice, the development of new data uses, the application of new observation methods and models, and estimating and accounting for uncertainty. Stakeholder engagement—why and how—is a topic that has increased in importance over time. While our observation methods did not change much over many decades, they are now changing rapidly due to new technological developments, but also societal and environmental changes.
Ambient fine particulate matter and daily mortality: a comparative analysis of observed and estimated exposure in 347 cities
Authors: Yu Wenhua , Huang Wenzhong , Gasparrini Antonio , Sera Francesco , Schneider Alexandra , Breitner Susanne , Kyselý Jan , Schwartz Joel , Madureira Joana , Gaio Vânia , Guo Yue Leon , Xu Rongbin , Chen Gongbo , Yang Zhengyu , Wen Bo , Wu Yao , Zanobetti Antonella , Kan Haidong , Song Jiangning , Li Shanshan , Guo Yuming ,   Multi-City Multi-Country Collaborative Research Network, Tong Shilu , Pascal Mathilde , das Neves Pereira da Silva Susana , Tobias Aurelio , Íñiguez Carmen , Pan Shih-Chun , Urban Aleš , Jaakkola Jouni J K , Ryti Niilo , Ameling Caroline , Rao Shilpa , Forsberg Bertil , Scortichini Matteo , Stafoggia Massimo , Masselot Pierre 
Published: May 2024

Abstract

Background
Model-estimated air pollution exposure products have been widely used in epidemiological studies to assess the health risks of particulate matter with diameters of ≤2.5 µm (PM2.5). However, few studies have assessed the disparities in health effects between model-estimated and station-observed PM2.5 exposures.

Methods
We collected daily all-cause, respiratory and cardiovascular mortality data in 347 cities across 15 countries and regions worldwide based on the Multi-City Multi-Country collaborative research network. The station-observed PM2.5 data were obtained from official monitoring stations. The model-estimated global PM2.5 product was developed using a machine-learning approach. The associations between daily exposure to PM2.5 and mortality were evaluated using a two-stage analytical approach.

Results
We included 15.8 million all-cause, 1.5 million respiratory and 4.5 million cardiovascular deaths from 2000 to 2018. Short-term exposure to PM2.5 was associated with a relative risk increase (RRI) of mortality from both station-observed and model-estimated exposures. Every 10-μg/m3 increase in the 2-day moving average PM2.5 was associated with overall RRIs of 0.67% (95% CI: 0.49 to 0.85), 0.68% (95% CI: –0.03 to 1.39) and 0.45% (95% CI: 0.08 to 0.82) for all-cause, respiratory, and cardiovascular mortality based on station-observed PM2.5 and RRIs of 0.87% (95% CI: 0.68 to 1.06), 0.81% (95% CI: 0.08 to 1.55) and 0.71% (95% CI: 0.32 to 1.09) based on model-estimated exposure, respectively.

Conclusions
Mortality risks associated with daily PM2.5 exposure were consistent for both station-observed and model-estimated exposures, suggesting the reliability and potential applicability of the global PM2.5 product in epidemiological studies.
The silent threat: investigating sleep disturbances in hospitalized patients
Authors: Adams Corey , Harrison Reema , Schembri Anthony , Junge Moira , Walpola Ramesh 
Published: May 2024

Abstract
Sleep disruptions in the hospital setting can have adverse effects on patient safety and well-being, leading to complications like delirium and prolonged recovery. This study aimed to comprehensively assess the factors influencing sleep disturbances in hospital wards, with a comparison of the sleep quality of patients staying in single rooms to those in shared rooms. A mixed-methods approach was used to examine patient-reported sleep quality and sleep disruption factors, in conjunction with objective noise measurements, across seven inpatient wards at an acute tertiary public hospital in Sydney, Australia. The most disruptive factor to sleep in the hospital was noise, ranked as ‘very disruptive’ by 20% of patients, followed by acute health conditions (11%) and nursing interventions (10%). Patients in shared rooms experienced the most disturbed sleep, with 51% reporting ‘poor’ or ‘very poor’ sleep quality. In contrast, only 17% of the patients in single rooms reported the same. Notably, sound levels in shared rooms surpassed 100 dB, highlighting the potential for significant sleep disturbances in shared patient accommodation settings. The results of this study provide a comprehensive overview of the sleep-related challenges faced by patients in hospital, particularly those staying in shared rooms. The insights from this study offer guidance for targeted healthcare improvements to minimize disruptions and enhance the quality of sleep for hospitalized patients.
AI IR: Charting International Relations in the Age of Artificial Intelligence
Authors: Baele Stephane J , Bukhari Iqraa , Whyte Christopher , Cuomo Scott , Jensen Benjamin , Payne Kenneth , Garcia Eugenio V 
Published: May 2024

Abstract
Over the past decade, rapid progress in artificial intelligence (AI) has transformed a range of areas, from medicine to strategic games and communication technologies, from art and culture to everyday office work. It would be naïve to assume that this evolution does not permeate and alter international affairs. Building on, and solidifying, a thriving yet still fragmented emerging literature on “AI IR,” this forum gathers several critical diagnoses of the way AI technologies impact on various areas of international relations. Introducing new concepts and charting emerging empirical realities, contributors explore how AI advances, such as autonomous lethal systems, synthetic imagery and text, or intelligent systems, are already creating new landscapes of violent and nonviolent international interactions. Yet, behind their distinct takes, contributions together stress the need to correctly locate and evaluate specific sites of AI impact, thus offering a nuanced appraisal scrutinizing grand declarations of an “AI revolution” in global politics.
Skin pharmacokinetics of miltefosine in the treatment of post-kala-azar dermal leishmaniasis in South Asia
Authors: Palić Semra , Chu Wan-Yu , Sundar Shyam , Mondal Dinesh , Das Pradeep , Pandey Krishna , Raja Sheeraz , Rijal Suman , Roseboom Ignace C , Hamadeh Abdullah , Malik Paul R V , Beijnen Jos H , Huitema Alwin D R , Sjögren Erik , Alves Fabiana , Dorlo Thomas P C 
Published: May 2024

Abstract

Introduction
Post-kala-azar dermal leishmaniasis (PKDL) arises as a dermal complication following a visceral leishmaniasis (VL) infection. Current treatment options for PKDL are unsatisfactory, and there is a knowledge gap regarding the distribution of antileishmanial compounds within human skin. The present study investigated the skin distribution of miltefosine in PKDL patients, with the aim to improve the understanding of the pharmacokinetics at the skin target site in PKDL.

Methods
Fifty-two PKDL patients underwent treatment with liposomal amphotericin B (20 mg/kg) plus miltefosine (allometric dosing) for 21 days. Plasma concentrations of miltefosine were measured on study days 8, 15, 22 and 30, while a punch skin biopsy was taken on day 22. A physiologically based pharmacokinetic (PBPK) model was developed to evaluate the distribution of miltefosine into the skin.

Results
Following the allometric weight-based dosing regimen, median miltefosine concentrations on day 22 were 43.73 µg/g (IQR: 21.94–60.65 µg/g) in skin and 33.29 µg/mL (IQR: 25.9–42.58 µg/mL) in plasma. The median individual concentration ratio of skin to plasma was 1.19 (IQR: 0.79–1.9). In 87% (45/52) of patients, skin exposure was above the suggested EC90 PK target of 10.6 mg/L associated with in vitro susceptibility. Simulations indicated that the residence time of miltefosine in the skin would be more than 2-fold longer than in plasma, estimated by a mean residence time of 604 versus 266 hours, respectively.

Conclusion
This study provides the first accurate measurements of miltefosine penetration into the skin, demonstrating substantial exposure and prolonged retention of miltefosine within the skin. These findings support the use of miltefosine in cutaneous manifestations of leishmaniasis. In combination with parasitological and clinical data, these results are critical for the future optimization of combination therapies with miltefosine in the treatment of PKDL.
Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
Authors: Klomchit Anthikan , Calabon Mark S , Worabandit Sompradtana , Weaver Jack A , Karima Elfina M , Alberti Fabrizio , Greco Claudio , Mahanil Siraprapa 
Published: May 2024

Abstract

Aims
Neocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis.

Methods and results
Detailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17–0253, MFLUCC 17–0257, and MFLUCC 17–0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17–0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17–0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17–0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters.

Conclusion
Two fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17–0253 were obtained.
A novel N-acetylglucosamine-6-phosphate deacetylase that is essential for chitin utilization in the chitinolytic bacterium, Chitiniphilus shinanonensis
Authors: Ichioka Ryotaro , Kitazawa Yuri , Taguchi Goro , Shimosaka Makoto 
Published: May 2024

Abstract

Aims
We aimed to investigate the function of an unidentified gene annotated as a PIG-L domain deacetylase (cspld) in Chitiniphilus shinanonensis SAY3. cspld was identified using transposon mutagenesis, followed by negatively selecting a mutant incapable of growing on chitin, a polysaccharide consisting of N-acetyl-d-glucosamine (GlcNAc). We focused on the physiological role of CsPLD protein in chitin utilization.

Methods and results
Recombinant CsPLD expressed in Escherichia coli exhibited GlcNAc-6-phosphate deacetylase (GPD) activity, which is involved in the metabolism of amino sugars. However, SAY3 possesses two genes (csnagA1 and csnagA2) in its genome that code for proteins whose primary sequences are homologous to those of typical GPDs. Recombinant CsNagA1 and CsNagA2 also exhibited GPD activity with 23 and 1.6% of catalytic efficiency (kcat/Km), respectively, compared to CsPLD. The gene-disrupted mutant, Δcspld was unable to grow on chitin or GlcNAc, whereas the three mutants, ΔcsnagA1, ΔcsnagA2, and ΔcsnagA1ΔcsnagA2 grew similarly to SAY3. The determination of GPD activity in the crude extracts of each mutant revealed that CsPLD is a major enzyme that accounts for almost all cellular activities.

Conclusions
Deacetylation of GlcNAc-6P catalyzed by CsPLD (but not by typical GPDs) is essential for the assimilation of chitin and its constituent monosaccharide, GlcNAc, as a carbon and energy source in C. shinanonensis.
IGF2BP2 regulates the proliferation and migration of endometrial stromal cells through the PI3K/AKT/mTOR signaling pathway in Hu sheep
Authors: Deng Kaiping , Li Xiaodan , Liu Zhipeng , Su Yalong , Sun Xuan , Wei Wurilege , Fan Yixuan , Zhang Yanli , Wang Feng 
Published: May 2024

Abstract
Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), a significant member of the conserved RNA-binding protein family, plays various roles in numerous physiological and pathological processes. However, the specific function of IGF2BP2 in regulating endometrial function in sheep remains largely unknown. In this study, we observed a significant upregulation in IGF2BP2 mRNA abundance in the endometrium during the luteal phase compared to the follicular phase in Hu sheep. The knockdown of IGF2BP2 resulted in accelerated cell proliferation and migration of Hu sheep endometrial stromal cells (ESCs). Moreover, RNA sequencing analysis revealed that genes with significantly altered expression in IGF2BP2 knockdown cells were predominantly enriched in endometrial receptivity-related signaling pathways, such as cytokine-cytokine receptor interaction, NOD-like receptor, PI3K-AKT, and JAK-STAT signaling pathway. Additionally, the knockdown of IGF2BP2 significantly increased the expression of matrix metalloprotein 9 (MMP9), vascular endothelial growth factor, and prolactin (PRL) in ESCs. The knockdown of IGF2BP2 was also observed to stimulate the PI3K/AKT/mTOR pathway by upregulating integrin β4 (ITGB4) expression. Notably, the downregulation of ITGB4 attenuates IGF2BP2 knockdown-induced facilitation of proliferation and migration of Hu sheep ESCs by inhibiting the PI3K/AKT/mTOR pathway. Collectively, these findings highlight the important role of IGF2BP2 in regulating endometrial function, particularly through the modulation of ESC proliferation and migration via the PI3K/AKT/mTOR pathway.
Production and characterization of novel monoclonal antibodies against pathological human TDP-43 proteins
Authors: Zheng Xiaojing , Wang Mengtian , He Qiongyan , Chen Shuyu , Simayi Dilihumaer , Ma Xia , Zhao Junli , Sun Xiaohong , Yang Peiyan , Mao Qinwen , Xia Haibin 
Published: May 2024

Abstract
The RNA/DNA-binding protein TDP-43 plays a pivotal role in the ubiquitinated inclusions characteristic of TDP-43 proteinopathies, including most cases of frontotemporal lobar degeneration (FTLD-TDP) and Alzheimer disease (AD). To understand the mechanisms of pathological TDP-43 processing and identify potential biomarkers, we generated novel phosphorylation-independent monoclonal antibodies (MAbs) using bacteria-expressed human full-length recombinant TDP-43. Remarkably, we identified a distinctive MAb, No. 9, targeting an epitope in amino acid (aa) region 311–360 of the C-terminus. This antibody showed preferential reactivity for pathological TDP-43 inclusions, with only mild reactivity for normal nuclear TDP-43. MAb No. 9 revealed more pathology in FTLD-TDP type A and type B brains and in AD brains compared to the commercial p409/410 MAb. Using synthetic phosphorylated peptides, we also obtained MAbs targeting the p409/410 epitope. Interestingly, MAb No. 14 was found to reveal additional pathology in AD compared to the commercial p409/410 MAb, specifically, TDP-43-immunopositive deposits with amyloid plaques in AD brains. These unique immunopositivities observed with MAbs No. 9 and No. 14 are likely attributed to their conformation-dependent binding to TDP-43 inclusions. We expect that this novel set of MAbs will prove valuable as tools for future patient-oriented investigations into TDP-43 proteinopathies.
Nurse Attrition: Content Analysis of Free-Text Responses From Two Military Nursing Practice Environment Surveys
Authors: Miller Melissa J , Korbut Nickalous , Amador Garcia Lidilia , Agazio Janice B , Cartwright Joel 
Published: May 2024

ABSTRACT

Introduction
Staff nurse attrition negatively impacts readiness of the warfighter and the health and wellbeing of all beneficiaries of the Military Health System (MHS). To promote the retention of a robust nursing workforce, a complete understanding of nurses’ perceptions regarding their intent to leave is necessary. The purpose of this study was to explore the free-text responses of 1,438 nurses working among military medical treatment facilities for factors influencing their intent to leave, as an indicator of potential turnover, or attrition.

Materials and Methods
This study employed thematic analysis to investigate the qualitative responses from the 2016 and 2018 Military Nursing Practice Environment Surveys. The study was determined to be exempt from Institutional Review Board review through the Womack Army Medical Center’s Human Research Protection Program.

Results
Findings indicate that leadership and management (n = 647), staffing (n = 353), career opportunities (n = 345), staff outcomes (n = 247), culture (n = 153), quality of care (n = 99), patient care barriers (n = 86), non-patient care activities (n = 79), lack of formal professional development (n = 75), and area or care environment (n = 67) were among the top factors influencing staff nurse intent to leave, respectively.

Conclusions
Our findings support the evaluation of retention strategies using implementation science for nurses and nurse resource personnel (e.g., nursing assistants, technicians, medics, and corpsman) to ensure a robust nursing work force throughout the MHS. Staff nurses and resource personnel working among military treatment facilities and embedded in units supporting combat and humanitarian missions ensure access to care and health promotion of the warfighter and all MHS beneficiaries.
DEVILS/MIGHTEE/GAMA/DINGO: the impact of SFR time-scales on the SFR-radio luminosity correlation
Authors: Cook Robin H W , Davies Luke J M , Rhee Jonghwan , Hale Catherine L , Bellstedt Sabine , Thorne Jessica E , Delvecchio Ivan , Collier Jordan D , Dodson Richard , Driver Simon P , Holwerda Benne W , Jarvis Matt J , Knowles Kenda , Lagos Claudia , Maddox Natasha , Meyer Martin , Robotham Aaron S G , Roychowdhury Sambit , Rozgonyi Kristof , Seymour Nicholas , Siudek Malgorzata , Whiting Matthew , Whittam Imogen 
Published: May 2024

ABSTRACT
The tight relationship between infrared luminosity (LTIR) and 1.4 GHz radio continuum luminosity ($L_\mathrm{1.4\, GHz}$) has proven useful for understanding star formation free from dust obscuration. Infrared emission in star-forming galaxies typically arises from recently formed, dust-enshrouded stars, whereas radio synchrotron emission is expected from subsequent supernovae. By leveraging the wealth of ancillary far-ultraviolet – far-infrared photometry from the Deep Extragalactic VIsible Legacy Survey and Galaxy and Mass Assembly surveys, combined with 1.4 GHz observations from the Meer Karoo Array Telescope International GHz Tiered Extragalactic Exploration survey and Deep Investigation of Neutral Gas Origin projects, we investigate the impact of time-scale differences between far-ultraviolet – far-infrared and radio-derived star formation rate (SFR) tracers. We examine how the spectral energy distribution (SED)-derived star formation histories (SFHs) of galaxies can be used to explain discrepancies in these SFR tracers, which are sensitive to different time-scales. Galaxies exhibiting an increasing SFH have systematically higher LTIR and SED-derived SFRs than predicted from their 1.4 GHz radio luminosity. This indicates that insufficient time has passed for subsequent supernovae-driven radio emission to accumulate. We show that backtracking the SFR(t) of galaxies along their SED-derived SFHs to a time several hundred megayears prior to their observed epoch will both linearize the SFR–$L_\mathrm{1.4\, GHz}$ relation and reduce the overall scatter. The minimum scatter in the SFR(t)–$L_\mathrm{1.4\, GHz}$ is reached at 200 – 300 Myr prior, consistent with theoretical predictions for the time-scales required to disperse the cosmic ray electrons responsible for the synchrotron emission.
Tannic acid and silicate-functionalized polyvinyl alcohol–hyaluronic acid hydrogel for infected diabetic wound healing
Authors: Diao Zhentian , Li Longkang , Zhou Huan , Yang Lei 
Published: May 2024

Abstract
Healing of chronic diabetic wounds is challenging due to complications of severe inflammatory microenvironment, bacterial infection and poor vascular formation. Herein, a novel injectable polyvinyl alcohol–hyaluronic acid-based composite hydrogel was developed, with tannic acid (TA) and silicate functionalization to fabricate an ‘all-in-one’ hydrogel PTKH. On one hand, after being locally injected into the wound site, the hydrogel underwent a gradual sol–gel transition in situ, forming an adhesive and protective dressing for the wound. Manipulations of rheological characteristics, mechanical properties and swelling ability of PTKH could be performed via regulating TA and silicate content in hydrogel. On the other hand, PTKH was capable of eliminating reactive oxygen species overexpression, combating infection and generating a cell-favored microenvironment for wound healing acceleration in vitro. Subsequent animal studies demonstrated that PTKH could greatly stimulate angiogenesis and epithelization, accompanied with inflammation and infection risk reduction. Therefore, in consideration of its impressive in vitro and in vivo outcomes, this ‘all-in-one’ multifunctional hydrogel may hold promise for chronic diabetic wound treatment.