bluuebunny/crossref_metadata_2025_split huggingface dataset:
An analysis of five clear cell papillary cystadenomas of mesosalpinx and broad ligament: four associated with von Hippel‐Lindau disease and one aggressive sporadic type
Authors: Nogales Francisco F , Goyenaga Pablo , Preda Ovidiu , Nicolae Alina , Vieites Begoña , Ruiz‐Marcellan Maria Carmen , Pedrosa Alberto , Merino Maria J 
Published: February 2012

Nogales F F, Goyenaga P, Preda O, Nicolae A, Vieites B, Ruiz‐Marcellan M C, Pedrosa A & Merino M J  (2012) Histopathology 60, 748–757 An analysis of five clear cell papillary cystadenomas of mesosalpinx and broad ligament: four associated with von Hippel‐Lindau disease and one aggressive sporadic typeAims:  Clear cell papillary cystadenoma (CCPC) is associated with von Hippel‐Lindau disease (VHLD), but rarely involves mesosalpinx and broad ligament (M/BL). This study provides new data about its behaviour and immunophenotype.Methods and results:  We performed an analysis of four benign cases of CCPC of M/BL with either characteristic clinical features or genetic markers [loss of heterozygosity (LOH)] of VHLD in patients ranging from 24 to 36 years and a sporadic case in a 52‐year‐old presenting with peritoneal metastases. All CCPCs were papillary but had solid and tubular areas. Haemorrhage, thrombosis and scarring were constant features and related to an unusual pattern of sub‐epithelial vascularity. All clear or oxyphilic cells co‐expressed cytokeratin 7 (CK7), CAM5.2 and vimentin, with strong apical CD10 and nuclear paired box gene 2 (PAX2) immunoreactivity. Three cases also showed positivity for VHL40, epithelial membrane antigen (EMA), Wilms’ tumour suppressor gene (WT‐1) and cancer antigen 125 (CA125) but only one expressed renal cell carcinoma (RCC) antigen. Vascular plexus overexpressed nuclear and cytoplasmic WT‐1.Conclusion:  The VHLD‐associated cases appeared to be benign, but the sporadic case exhibited a low malignant potential. CCPCs show histological and immunophenotypical similarities with the recently reported clear cell papillary RCC, although the previously unreported apical CD10 and nuclear PAX2 expression may be related to their mesonephric origin. CCPC has a distinctive sub‐epithelial vascular pattern that is consistent with its pathogenesis.
Children’s experiences of participation in the cleft lip and palate care pathway
Authors: HALL MELANIE , GIBSON BARRY , JAMES ALLISON , RODD HELEN D. 
Published: February 2012

International Journal of Paediatric Dentistry 2012; 22: 442–450Aim.  This qualitative study sought to explore children’s perspectives on their participation in the cleft lip and palate care pathway.Design.  Eight boys and nine girls (aged 8–17 years), with a range of cleft types and who were patients at a British dental hospital each took part in two child‐centred interviews which incorporated participatory activities. An initial interview focused on children’s general life stories, and these often encompassed a discussion about cleft lip and/or palate. A follow‐up interview explored specific aspects of the condition and its related treatment.Results.  Data revealed the varying roles that young people can play in decision‐making, which can be described as active or passive. In addition, the dynamic degree of participation was highlighted with patients occupying different roles throughout the care pathway.Conclusion.  The research provides an insight into treatment decisions, and how young people, their families, and clinicians interact to arrive at these. Findings provide further evidence to support the important contribution young patients can make in their own treatment choices.
Association between parent reports of attention deficit hyperactivity disorder behaviours and child impulsivity in children with severe intellectual disability
Authors: Bigham K. , Daley D. M. , Hastings R. P. , Jones R. S. P. 
Published: February 2012

AbstractBackground  Although children with intellectual disability (ID) seemed to be at increased risk for Attention deficit hyperactivity disorder (ADHD)/hyperactivity problems when assessed with parent report questionnaires and clinical interviews, there has been little attention to the associations between parent reports and observed child behaviours. The purpose of the present study was to compare clinical symptoms and observed impulsivity in children with ID whose parents reported them as being relatively high and low in ADHD symptoms, and to examine whether any differences were associated with developmental level.Methods  Parents of 28 children with ID completed a behaviour rating scale of hyperactivity symptoms. Parents were also interviewed using a robust clinical interview tool focused on hyperactivity symptoms. The children were all tested by an experimenter to measure their impulsive behaviour.Results  Those children with clinical range scores on parent questionnaire ratings were also reported by parents to have more ADHD symptoms using a parent report clinical interview. Although these children were also more impulsive on an experimental task, when children's developmental ages were statistically controlled impulsivity differences disappeared.Conclusions  Parent reports of ADHD symptoms in children with ID may be positively associated with data derived using clinical interview methods, but they may be less sensitive to developmental expectations when compared with observed child behaviour. Practical implications include the need for multiple sources of information and normative data for children with ID on simple experimental tasks that can be used to aid diagnosis of ADHD in clinical settings.
Investigating low adaptive behaviour and presence of the triad of impairments characteristic of autistic spectrum disorder as indicators of risk for challenging behaviour among adults with intellectual disabilities
Authors: Felce D. , Kerr M. 
Published: February 2012

AbstractBackground  Identification of possible personal indicators of risk for challenging behaviour has generally been through association in cross‐sectional prevalence studies, but few analyses have controlled for intercorrelation between potential risk factors. The aim was to investigate the extent to which gender, age, presence of the triad of impairments characteristic of autism and level of adaptive behaviour were independently associated with level of challenging behaviour among adults with intellectual disabilities.Methods  Five datasets were merged to produce information on challenging behaviour, adaptive behaviour, presence of the triad of impairments, gender and age of 818 adults. Variables were entered into a multivariate linear regression, which also tested the interaction between the presence of the triad of impairments and level of adaptive behaviour.Results  Presence of the triad of impairments, level of adaptive behaviour, their interaction, and age, but not gender, significantly and independently contributed to the prediction of challenging behaviour. Presence/absence of the triad of impairments moderated the effect of adaptive behaviour on challenging behaviour. The inverse relationship found in the absence of the triad of impairments was virtually removed when present.Conclusions  This study has shown that it is necessary to control for intercorrelation between potential risk factors for challenging behaviour and to explore how interaction between them might moderate associations.
Relational and sexual fluidity in females partnered with male‐to‐female transsexual persons
Authors: ARAMBURU ALEGRÍA C. 
Published: February 2012

Accessible summary
Healthcare providers are in a privileged position to counsel and offer resources to patients who identify as sexually or gender‐diverse, or who are in relationships with sexually or gender‐diverse persons. To optimize health outcomes in these persons, a call has been made to increase providers' awareness of sexually diverse populations.
This research builds upon existing knowledge on female sexuality through its examination of female sexuality in relationships in which one partner discloses as male‐to‐female transsexual.
Sexual and relational orientation can be fluid, and can evolve within a relationship. Females partnered with male‐to‐female transsexual persons reported reforming their sexual relations with their partners. They reported maintaining a heterosexual identity, yet reconceptualizing their relationships and sexuality to be situation‐dependent. For example, language such as ‘situational lesbian’ was reported. The study findings also demonstrate that female sexuality within these relationships can range from cessation of sexual relations to a continuation of relations without disruption.
Healthcare providers need to be aware of fluidity and diversity among sexuality and relationships, and allow for expression of an expansive range of relationships and/or sexual activity.
AbstractThis paper reports on a study examining sexuality in females who remain partnered with male‐to‐female transsexual persons. Participants' self‐view and sexual fluidity following their partners' transition from man to woman is examined. Sixteen females participated in in‐depth, semi‐structured interviews. An inductive process of data analysis was conducted, using the constant comparative method, an iterative process by which data are compared within and across subjects. Data were collected until thematic saturation was achieved. Four themes related to sexuality emerged: (1) questioning of sexual orientation; (2) sexual orientation categorization; (3) relational fluidity without sexual relations; and (4) relational fluidity with sexual relations. Participants maintained a heterosexual identity, yet modified their self‐view to include an identity that reflected their reformed relationship. The majority of the respondents reported sexual lives that were active or evolving. Others remained in relationships that no longer included sexual activity. The study findings highlight the potential fluidity within the sexual and relational lives of females, and can enhance healthcare providers' preparedness and efficacy with diverse populations. Providers are in a unique position to offer resources to patients who identify as sexually or gender‐diverse, or who are in relationships with sexually or gender‐diverse persons.
Ca2+ sensitivity of the maxi chloride channel in interstitial cells of Cajal
Authors: Wright G. W. J. , Parsons S. P. , Huizinga J. D. 
Published: February 2012

AbstractBackground  Interstitial cells of Cajal (ICC) associated with the myenteric plexus of the small intestine express maxi chloride channels. Our aim was to investigate whether or not these channels would be activated by increases in intracellular Ca2+, as that would strengthen evidence for their potential role in ICC pacemaking. A further aim was to examine whether inwardly and outwardly rectifying maxi chloride currents signify different channels.Methods  We used Fluo‐4 AM Ca2+ imaging and patch clamp electrophysiology (cell‐attached and inside‐out) on isolated ICC in short term culture.Key Results  Increasing intracellular Ca2+ by three functionally distinct mechanisms (blocking sarcoplasmic reticulum Ca2+ refilling, creating membrane Ca2+ pores and a solution designed to block plasmalemmal Ca2+ extrusion) was followed by inwardly rectifying maxi chloride channel activation assessed in the cell‐attached configuration. Furthermore, in the inside‐out configuration, increased outwardly rectifying maxi‐chloride channel activity followed an increase in Ca2+ to 2 mmol L−1 at the cytoplasmic face of the channel.Conclusions & Inferences  Increase in intracellular Ca2+ will activate the maxi chloride channels. Maxi chloride currents are inwardly rectifying in the cell‐attached patch clamp configuration under physiological conditions and are outwardly rectifying in the inside‐out configuration. The same channel is responsible for both currents. Ca2+ does not appear to regulate the rectification.
Optimisation of mesenchymal stromal cells karyotyping analysis: implications for clinical use
Authors: Muntión S. , Sánchez‐Guijo F. M. , Carrancio S. , Villarón E. , López O. , Diez‐Campelo M. , San Miguel J. F. , del Cañizo M. C. 
Published: February 2012

Purpose: The aim of this study was to optimise the yield of metaphases in mesenchymal stromal cells (MSC) in vitro cultures and to study the karyotype of MSC expanded in good manufacturing practice (GMP) conditions for clinical use.Background: MSC are being increasingly used in clinical trials for a number of diseases. Biosafety demonstration in all cases is mandatory. Unfortunately, current standard karyotyping methods fail to obtain enough number of evaluable metaphases.Methods and materials: In the present work, to optimise the yield of metaphases in MSC expanded in vitro, we have tested several conditions by modifying colcemid concentration (we have tested 0·01, 0·05 and 0·1 µg mL−1) and exposure time (during 5, 15 and 24 h). We further applied these optimised conditions to 61 MSC expansions in GMP conditions for clinical use.Results: Our results show that the highest number of metaphases was obtained when MSC were incubated with 0·05 µg mL−1 of colcemid overnight (15 h), compared to the remaining experimental conditions. In most cases (59/61 cases) enough number of metaphases was obtained. And what is more relevant, only in one case a karyotypic abnormality was found (trisomy of chromosome 10), and cells were subsequently discarded for clinical use.Conclusion: We describe here an optimal method to obtain enough number of metaphases for karyotype analysis of in vitro expanded MSCs, what is essential for their clinical use in cell therapy programmes.
Correlation of Pfg377 ortholog gene expression of Plasmodium vivax and mosquito infection
Authors: Chansamut N. , Buates S. , Takhampunya R. , Udomsangpetch R. , Bantuchai S. , Sattabongkot J. 
Published: February 2012

AbstractObjective  To determine the expression of Pfg377 ortholog gene in Plasmodium vivax, and examine its correlation with mosquito infection.Methods  Seventy clinical blood samples positive for P. vivax by microscopy, were used for the mosquito infectivity assay. Infectivity to female Anopheles dirus was determined from oocyst counts. The transcripts of Pfg377 ortholog gene of P. vivax from blood samples infective and non‐infective to mosquitoes were examined using quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR).Results  Of 70 P. vivax positive blood samples, 50 (71.4%) samples were mosquito‐infective and 20 (28.6%) were not. In infective samples, the expression level of Pfg377 ortholog gene was significantly higher than in the non‐infective group (P < 0.05). In infective samples, the expression level of Pfg377 ortholog gene at ≥100 copies/ml of blood cut‐off point correlated with ≥10 oocysts/mosquito cut‐off point of average oocyst numbers and with ≥50% cut‐off point of per cent infected mosquitoes (Pearson’s chi‐square correlation, P = 0.014 and P = 0.026, respectively).Conclusion  The cut‐off point of the expression level of Pfg377 ortholog gene could be used to predict the infectiousness of P. vivax gametocytes leading to mosquito infection and parasite transmission in the field.
Public health services, an essential determinant of health during crisis. Lessons from Cuba, 1989–2000
Authors: De Vos Pol , García‐Fariñas Anaí , Álvarez‐Pérez Adolfo , Rodríguez‐Salvá Armando , Bonet‐Gorbea Mariano , Van der Stuyft Patrick 
Published: February 2012

AbstractDuring the 1990s, Cuba was able to overcome a severe crisis, almost without negative health impacts. This national retrospective study covering the years 1989–2000 analyses the country’s strategy through essential social, demographic, health process and health outcome indicators. Gross domestic product (GDP) diminished by 34.76% between 1989 and 1993. In 1994 slow recuperation started. During the crisis, public health expenses increased. The number of family doctors rose from 9.22 to 27.03 per 104 inhabitants between 1989 and 2000. Infant mortality rate and life expectancy exemplify a series of health indicators that continued to improve during the crisis years, whereas low birth weight and tuberculosis incidence are among the few indicators that suffered deterioration. GDP is inversely related to tuberculosis incidence, whereas the average salary is inversely related to low birth weight. Infant mortality rate has a strong negative correlation with the health expenses per inhabitant, the number of maternal homes, the number of family doctors and the proportion of pregnant women receiving care in maternal homes. Life expectancy has a strong positive correlation with health expenses, the number of nursing personnel and the number of medical contacts per inhabitant. The Cuban strategy effectively resolved health risks during the crisis. In times of serious socio‐economic constraints, a well conceptualized public health policy can play an important role in maintaining the overall well‐being of a population.
Evaluation of the diagnostic accuracy of the Haemoglobin Colour Scale to detect anaemia in young children attending primary healthcare clinics in Zanzibar
Authors: Aldridge Catherine , Foster Hamish M. E. , Albonico Marco , Ame Shaali M. , Montresor Antonio 
Published: February 2012

AbstractObjectives  This study evaluates the diagnostic accuracy of Haemoglobin Colour Scale (HCS), compared with clinical diagnosis, to detect anaemia and severe anaemia in preschool‐age children attending primary healthcare clinics in rural Zanzibar.Methods  In all participants, haemoglobin (Hb) concentration was independently estimated by clinical examination for palmar pallor, HCS and HemoCue™. HemoCue was considered the reference method. Data collection was integrated into the usual health services and performed by local healthcare workers (HCWs). Sensitivity, specificity, positive and negative predictive values were calculated for HCS and clinical examination for palmar pallor. The limits of agreement between HCS and HemoCue, and inter‐observer variability for HCS, were also defined.Results  A total of 799 children age 2–59 months were recruited to the study. The prevalence of anaemia (Hb < 11 g/dl) and severe anaemia (<5 g/dl) were 71% and 0.8% respectively. The sensitivity of HCS to detect anaemia was 33% [95% confidence interval (CI) 29–36] and specificity was 87% (83–91). The sensitivity of HCS to detect severe anaemia was 14% (95% CI 0–58) and specificity was 100% (99–100). The sensitivity of palmar pallor to detect anaemia was low, but superior to HCS (58%vs. 33%, P < 0.001); specificity was inferior to HCS (55%vs. 87%, P < 0.001). There was no evidence of a difference in either sensitivity (P > 0.1) or specificity (P > 0.1) between HCS and palmar pallor to detect severe anaemia.Conclusions  Haemoglobin Colour Scale does not improve the capacity of HCWs to diagnose anaemia in this population. Accuracy is limited by considerable variability in the performances of test operators. However, optimizing the training protocol for those using the test may improve performance.
Resistance evaluation for herbicide resistance–endowing acetolactate synthase (ALS) gene mutations using Raphanus raphanistrum populations homozygous for specific ALS mutations
Authors: YU Q , HAN H , LI M , PURBA E , WALSH M J , POWLES S B 
Published: February 2012

Yu Q, Han H, Li M, Purba E, Walsh MJ & Powles SB (2012). Resistance evaluation for herbicide resistance–endowing acetolactate synthase (ALS) gene mutations using Raphanus raphanistrum populations homozygous for specific ALS mutations. Weed Research 52, 178–186.SummaryAcetolactate synthase (ALS)‐inhibiting herbicide resistance is common in Raphanus raphanistrum (wild radish) populations across the Western Australian (WA) grain belt. This study investigates the molecular and biochemical basis of ALS herbicide resistance in five R. raphanistrum populations. Five known ALS herbicide resistance–endowing mutations (Pro‐197‐Ala, Pro‐197‐Thr, Pro‐197‐Ser, Asp‐376‐Glu and Trp‐574‐Leu) were identified, and their resistance spectrum to ALS‐inhibiting herbicides was determined using purified populations individually homozygous for each mutation (except for Pro‐197‐Ala). Plants homozygous for ALS mutations at Pro‐197 were found to be cross‐resistant to ALS‐inhibiting sulfonylurea (SU) and triazolopyrimidine (TP) herbicides, while plants homozygous for Trp‐574‐Leu were resistant to SU, TP and imidazolinone (IMI) ALS herbicide classes. The Asp‐376‐Glu mutation is reported here for the first time in R. raphanistrum populations and characterised at both the whole‐plant and enzyme level. Plants homozygous for Asp‐376‐Glu were highly resistant to SU and TP herbicides, based on LD50 R/S ratios (>130 and 128 respectively) and I50 R/S ratios (170 and >110 respectively). In contrast, these plants were moderately resistant to the IMI imazamox (LD50 R/S ratio of 8, I50 R/S ratio of 3) and imazethapyr (I50 R/S ratio of 8) and susceptible to imazapyr (I50 R/S ratio of 0.76). A novel observation in this study is that resistance of homozygous Glu‐376 plants is associated with a remarkable growth reduction in the presence of the ALS herbicides tested, making early resistance diagnosis and management difficult.
Risk factors for patient‐reported medical errors in eleven countries
Authors: Schwappach David L. B. 
Published: February 2012

AbstractObjectives  The aim of this study was to identify common risk factors for patient‐reported medical errors across countries. In country‐level analyses, differences in risks associated with error between health care systems were investigated. The joint effects of risks on error‐reporting probability were modelled for hypothetical patients with different health care utilization patterns.Design  Data from the Commonwealth Fund’s 2010 lnternational Survey of the General Public’s Views of their Health Care System’s Performance in 11 Countries.Setting  Representative population samples of 11 countries were surveyed (total sample = 19 738 adults). Utilization of health care, coordination of care problems and reported errors were assessed. Regression analyses were conducted to identify risk factors for patients’ reports of medical, medication and laboratory errors across countries and in country‐specific models.Results  Error was reported by 11.2% of patients but with marked differences between countries (range: 5.4–17.0%). Poor coordination of care was reported by 27.3%. The risk of patient‐reported error was determined mainly by health care utilization: Emergency care (OR = 1.7, P < 0.001), hospitalization (OR = 1.6, P < 0.001) and the number of providers involved (OR three doctors = 2.0, P < 0.001) are important predictors. Poor care coordination is the single most important risk factor for reporting error (OR = 3.9, P < 0.001). Country‐specific models yielded common and country‐specific predictors for self‐reported error. For high utilizers of care, the probability that errors are reported rises up to P = 0.68.Conclusions  Safety remains a global challenge affecting many patients throughout the world. Large variability exists in the frequency of patient‐reported error across countries. To learn from others’ errors is not only essential within countries but may also prove a promising strategy internationally.