bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathologic study and outcome analysis of thyroid lymphomas: Experience from a tertiary cancer center
Authors: Katna Rakesh , Shet Tanuja , Sengar Manju , Menon Hari , Laskar Siddharth , Prabhash Kumar , D'Cruz Anil , Nair Reena 
Published: February 2012

AbstractBackgroundThe aim of this study was to review clinicopathologic presentations of patients diagnosed with thyroid lymphomas at a tertiary cancer center. Thyroid lymphomas represent less than 2% of all lymphomas.MethodsThe lymphoma clinic database was retrospectively reviewed to collect information on patients diagnosed with thyroid lymphomas. Tissue microarrays were constructed in 37 patients for evaluation of germinal center (CD10/bcl‐6) and activated B‐cell immunophenotype markers (FoxP1, Mum1).ResultsDuring 2000 to 2010, 64 of 5668 patients registered at our lymphoma clinic were diagnosed with thyroid lymphoma (1.7%). Complete response (CR) to treatment was seen in 80.7%. The germinal center immunophenotype and activated B‐cell immune phenotype did not influence the prognosis. FoxP1, however, was associated with poor treatment response and decreased survival.ConclusionsAdvanced International Prognostic Index (IPI) score and combined‐modality treatment emerged as significant prognostic factors for treatment response and overall survival. Immunophenotype expression of FoxP1 carries a poor prognosis in diffuse large B‐cell lymphoma as elsewhere. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Human papillomavirus genotype detection in recurrent respiratory papillomatosis (RRP) in Colombia
Authors: Sanchez Gloria I. , Jaramillo Roberto , Cuello Gustavo , Quintero Katherine , Baena Armando , O'Byrne Adriana , Reyes Antonio Jose , Santamaria Consuelo , Cuello Harold , Arrunategui Anamaria , Cortez Armando , Osorio German , Reina Julio Cesar , Quint Wim G. V. , Muñoz Nubia 
Published: February 2012

AbstractBackgroundKnowledge on human papillomavirus (HPV) genotype distribution in recurrent respiratory papillomatosis (RRP) is essential to assess the impact of HPV vaccine. It is provided information for Colombia.MethodsIn all, 189 RRP primary cases diagnosed between 1985 and 2009 were identified from 5 pathology laboratories of Cali and Medellin, Colombia. HPV DNA testing in 129 cases that fulfilled inclusion criteria (available paraffin blocks, amplifiable DNA, and confirmed histologic diagnosis of RRP) was performed by the SPF‐10/LiPA25 assay (version 1).ResultsOf all cases 36.1% were juvenile (<12 years old) and a majority of adults were males (p = .09); 95% of cases were HPV positive. HPV 6, 11, and 16 contributed to 69%, 27.1%, and 7.8% of all HPV positive cases. Twelve cases (9.3%) showed multiple infections; 8 of these were HPV 6 or 11 positive.ConclusionsHPV prophylactic vaccine including HPV 6 and 11 may have a major impact against RRP. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Sebaceous gland carcinoma of the head and neck: The Princess Margaret Hospital experience
Authors: Erovic Boban M. , Goldstein David P. , Kim Dae , Al Habeeb Ayman , Waldron John , Ghazarian Danny , Irish Jonathan C. 
Published: February 2012

AbstractBackgroundThe purpose of this study was to identify prognostic factors predicting outcomes in sebaceous gland carcinomas.MethodsWe conducted a retrospective medical chart review of patients with sebaceous carcinomas of periorbital (n = 33) and extraorbital sites (n = 13).ResultsPatients with periorbital tumors had higher recurrence rates than did patients with extraorbital tumors (64% vs 23%; p = .032). Patients who were older than 60 years (p = .035) and had lower eyelid tumors (p < .0001) had a lower disease‐free survival rate than did patients with upper eyelid tumors. Patients with sebaceous carcinomas had a high rate (60%) of occult lymph node metastases.ConclusionPeriorbital tumors are associated with poorer outcomes than are extraorbital tumors. Lower eyelid carcinomas have the worst prognosis and should be treated more aggressively. Our findings of a high incidence of occult neck disease and a high rate of regional recurrence in patients with sebaceous carcinomas support the consideration of prophylactic elective neck dissections for treating such patients. © 2012 Wiley Periodicals, Inc. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Targeting ErbB‐1 and ErbB‐4 in irradiated head and neck cancer: Results of in vitro and in vivo studies
Authors: Barnea Itay , Haif Sasha , Keshet Rom , Karaush Vicky , Lev‐Ari Shahar , Khafif Avi , Shtabsky Alexander , Yarden Yosef , Vexler Akiva , Yosef Rami Ben 
Published: February 2012

AbstractBackgroundErbB oncogenes have a major role in cancer. The role of ErbB‐4 in cancer cell biology and the effect of anti‐ErbB‐1 and anti‐ErbB‐4 monoclonal antibodies were evaluated in this study.MethodsErbB‐4 expression and binding was evaluated by Western blot, enzyme‐linked immunosorbent assay (ELISA), fluorescent microscopy, and flow cytometry. Cell survival was measured by XTT assay. Tumor progression was followed up in nude mice model.ResultsHigh ErbB‐1 levels in head and neck cancer cell lines were determined, whereas ErbB‐4 expression varied. Specific antibody binding to the cells was demonstrated. High ErbB‐4 expressing squamous cell carcinoma 1 (SCC‐1) cells proliferated faster and generated faster growing tumors in mice. Cetuximab and mAb‐3 reduced cell survival proportional to ErbB‐1 and ErbB‐4 expression. Combination of antibodies with irradiation was most effective in reducing cell survival and tumor growth.ConclusionErbB‐4 plays a role in head and neck cancer cell biology. Anti‐ErbB‐4 targeted therapy can serve as a new strategy against head and neck cancer when combined with established treatments. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Does osteoporosis therapy invalidate FRAX for fracture prediction?
Authors: Leslie William D , Lix Lisa M , Johansson Helena , Oden Anders , McCloskey Eugene , Kanis John A ,   for the Manitoba Bone Density Program
Published: February 2012

Abstract
Ten-year fracture risk assessment with the fracture risk assessment system (FRAX) is increasingly used to guide treatment decisions. Osteoporosis pharmacotherapy reduces fracture risk, but the effect is greater than can be explained from the increase in bone mineral density (BMD). Whether this invalidates fracture predictions with FRAX is uncertain. A total of 35,764 women (age ≥50 years) and baseline BMD testing (1996–2007) had FRAX probabilities retroactively calculated. A provincial pharmacy database was used to identify osteoporosis medication use. Women were categorized as untreated, current high adherence users [medication possession ratio (MPR) ≥0.80 in the year after BMD testing], current low adherence users (MPR &lt;0.80), and past users. Fractures outcomes to 10 years were established form a population-based health data repository. FRAX and femoral neck BMD alone stratified major osteoporotic and hip fracture risk within untreated and each treated subgroup (all p-values &lt;0.001) with similar area under the receiver operating characteristic curve. In untreated and each treated subgroup, a stepwise gradient in observed 10-year major osteoporotic and hip fracture incidence was found as a function of the predicted probability tertile (all p-values &lt;0.001 for linear trend). Concordance (calibration) plots for major osteoporotic fractures and hip fractures showed good agreement between the predicted and observed 10-year fracture incidence in untreated women and each treated subgroup. Only in the highest risk tertile of women highly adherent to at least 5 years of bisphosphonate use was observed hip fracture risk significantly less than predicted, though major osteoporotic fracture risk was similar to predicted. In summary, this work suggests that the FRAX tool can be used to predict fracture probability in women currently or previously treated for osteoporosis. Although FRAX should not be used to assess the reduction in fracture risk in individuals on treatment, it may still have value for guiding the need for continued treatment or treatment withdrawal. © 2012 American Society for Bone and Mineral Research.
Isolation, characterization and enzymatic modification of water soluble xylans from Eucalyptus grandis wood and sugarcane bagasse
Authors: Chimphango Annie F.A. , van Zyl Willem H. , Görgens Johann F. 
Published: February 2012

AbstractBACKGROUND: There is growing interest in utilizing xylans as biodegradable polymers to replace synthetic additives, coatings and encapsulation matrices. However, techniques are required to reduce the water solubility of the xylan for such applications. The study objective was to isolate and characterize water soluble xylans from Eucalyptus grandis wood and sugarcane (Saccharum officinarum L) bagasse for subsequent precipitation in water by enzymatic modification.RESULTS: The water soluble xylans were extracted from E. grandis and bagasse using two mild alkali‐low‐temperature extraction methods in which the xylan was recovered either by ultra‐purification (Hoije) or ethanol precipitation (Lopez). Yields of 66 and 35% were obtained for the xylan extracted from bagasse and E. grandis, respectively, using the Hoije method and 28 and 12%, respectively, using the Lopez method. The xylans were hydrolysed by selective removal of arabinose and 4‐O‐methylglucuronic acid (4‐O‐MeGlcA) side chains by treatment with α‐L‐arabinofuranosidase and α‐D‐glucuronidase, respectively. The α‐L‐arabinofuranosidase removed about 14% of the available arabinose in the xylans extracted from bagasse using the Hoije method, which led to precipitation in water. However, the α‐D‐glucuronidase removed only 2% 4‐O‐MeGlcA of the available 4‐O‐MeGlcA from the xylans extracted from bagasse and E. grandis using both the Hoije and Lopez methods, and failed to show visible precipitation of the xylan in water.CONCLUSION: The xylans extracted from bagasse suited the substrate specificities of the α‐L‐arabinofuranosidase for side chain removal and precipitation in water. However, the dosage of α‐D‐glucuronidase for precipitating the xylan would need to be increased. Copyright © 2012 Society of Chemical Industry
Bi‐RADS category 3 nonpalpable breast masses on sonography: long‐term results of a prospective cohort study
Authors: Alimoglu Emel , Alimoglu M. Kemal , Ceken Kagan , Kabaalioglu Adnan , Apaydin Ali , Cassano Enrico , Sindel Timur 
Published: February 2012

AbstractPurpose:To determine the frequency of BI‐RADS category 3 nonpalpable masses detected using only sonography (US) and the malignancy rate among these lesions. Second, to validate a proposed short‐term follow‐up regimen based on long‐term observational results.Methods:This prospective cohort study was conducted between September 2003 and April 2010. We conducted a 2‐year short‐term follow‐up protocol composed of five US examinations at 3‐month intervals for the first 6 months, and at 6‐month intervals for the next 18 months, followed with age‐appropriate screenings. Biopsy was recommended for the masses increasing in size and with changing imaging features.Results:The frequency of category 3 nonpalpable masses detected only on US in 11,373 consecutive women was 5.3%. Of 562 lesions found in 451 women, 394 (70.1%) remained stable during the short‐term and subsequent follow‐up. Seventy‐four (13.1%) masses showed interval regression and 79 (14.0%) showed interval progression. The malignancy rate was 0.3% (2 of 562), with about 85% of interval changes occurring within the first 2 years. The negative predictive value of US in the detection of cancer was 99.6% (95% CI, 98.7–99.9). The mean follow‐up time was 65.5 ± 8.7 months.Conclusions:The frequency of BI‐RADS 3 nonpalpable masses detected using sonography alone was 5.3%. During follow‐up, the majority of interval changes occurred within the first 2 years. Because these masses have a very low malignancy rate, a 2‐year follow‐up instead of immediate biopsy is an appropriate option. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2012;
Prenatal diagnosis and management of congenital chloride diarrhea: A case report of 2 siblings
Authors: Imada Shinya , Kikuchi Akihiko , Horikoshi Tsuguhiro , Ishikawa Kaori , Tamaru Shunsuke , Komatsu Atsushi , Takagi Kimiyo , Ogiso Yoshifumi 
Published: February 2012

AbstractCongenital chloride diarrhea (CLD) is a rare hereditary disease. The basic defect of CLD is massive loss of Cl− and fluid into the ileum and colon. Prenatal diagnosis of this disease is quite important because the infant requires electrolyte supplementation from the early postnatal period. Two cases in which prenatal diagnoses of CLD were made in siblings are reported. Extreme electrolyte imbalance may cause fetal cardiac dysfunction or a poor general condition leading to a non‐reassuring fetal status in cases with CLD. Therefore, frequent fetal monitoring using cardiotocograms and ultrasound may be beneficial to some fetuses with CLD to detect fetal deterioration. In addition, repeated amnioreduction may be required to treat severe polyhydramnios and threatened preterm delivery. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2012;
Bone marrow cells of swine: Collection and separation
Authors: Branco Érika , Cabral Rosa , Gomes Bruno Duarte , Kfoury José Roberto , Miglino Maria Angelica 
Published: February 2012

AbstractBone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.
Nutritive value of diets containing fish silage for juvenile Litopenaeus vannamei (Bonne, 1931)
Authors: Gallardo Pedro , Gaxiola Gabriela , Soberano Sofía , Taboada J Gabriel , Pérez Marquelia , Rosas Carlos , Cuzon Gerard , Espinosa L Gabriela , Sotelo Angela 
Published: February 2012

AbstractBACKGROUND: Fish wastes has been used for many years as an alternative in feeds for aquaculture. In the present study weight gain of juvenile white shrimp Litopenaeus vannamei fed diets including fish waste silage (WS), fish waste silage with soybean meal SBM (WS + S) or fish waste meal (WM) was compared. A conventional acidic silage process was applied to obtain from wastes (skin, heads, bones and viscera) of snapper (Lutjanus spp.), grunt (Haemulon plumieri), and grouper (Epinephelus spp.) an ingredient rich in protein.RESULTS: After 3 days ensilage more than 90% protein was hydrolysed. Waste material processed at pH 3.8 lost about 24% tryptophan. Butylated hydroxytoluene (BHT) prevented lipid oxidation, as shown after 45 days with malonaldehyde production. Shrimp fed WS + S diet gained 0.7 g per week higher than those fed WS and WM diets with 0.3 g per week (P < 0.05).CONCLUSION: WS processed with formic acid under conditions of low pH is beneficial for the white shrimp L. vannamei. It sustained reasonable weight gain combined with soybean meal in practical diets. On the other hand, BHT addition was beneficial in preventing oxidative action during silage preparation. Copyright © 2012 Society of Chemical Industry
In vivo 31P MR spectroscopic imaging of the human prostate at 7 T: Safety and feasibility
Authors: Kobus Thiele , Bitz Andreas K. , van Uden Mark J. , Lagemaat Miriam W. , Rothgang Eva , Orzada Stephan , Heerschap Arend , Scheenen Tom W. J. 
Published: February 2012

Abstract31P MR spectroscopic imaging of the human prostate provides information about phosphorylated metabolites that could be used for prostate cancer characterization. The sensitivity of a magnetic field strength of 7 T might enable 3D 31P MR spectroscopic imaging with relevant spatial resolution in a clinically acceptable measurement time. To this end, a 31P endorectal coil was developed and combined with an eight‐channel 1H body‐array coil to relate metabolic information to anatomical location. An extensive safety validation was performed to evaluate the specific absorption rate, the radiofrequency field distribution, and the temperature distribution of both coils. This validation consisted of detailed Finite Integration Technique simulations, confirmed by MR thermometry and B measurements in a phantom and in vivo temperature measurements. The safety studies demonstrated that the presence of the 31P endorectal coil had no influence on the specific absorption rate levels and temperature distribution of the external eight‐channel 1H array coil. To stay within a 10 g averaged local specific absorption rate of 10 W/kg, a maximum time‐averaged input power of 33 W for the 1H array coil was allowed. For transmitting with the 31P endorectal coil, our safety limit of less than 1°C temperature increase in vivo during a 15‐min MR spectroscopic imaging experiment was reached at a time‐averaged input power of 1.9 W. With this power setting, a second in vivo measurement was performed on a healthy volunteer. Using adiabatic excitation, 3D 31P MR spectroscopic imaging produced spectra from the entire prostate in 18 min with a spatial resolution of 4 cm3. The spectral resolution enabled the separate detection of phosphocholine, phosphoethanolamine, inorganic phosphate, and other metabolites that could play an important role in the characterization of prostate cancer. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.
The Role of Preoperative Self‐Efficacy in Predicting Outcome after Total Knee Replacement
Authors: Wylde V. , Dixon S. , Blom A.W. 
Published: February 2012

AbstractObjectivesThe aim of the present study was to determine if self‐efficacy is a significant and independent preoperative predictor of patient‐reported pain and function at one year after total knee replacement (TKR).MethodsPatients listed for a primary TKR because of osteoarthritis were recruited from preoperative assessment clinics at one regional orthopaedic centre. Before surgery, patients completed the Western Ontario and McMasters Osteoarthritis Index (WOMAC) Pain and Function Scale, Pain Self‐Efficacy Scale, the Hospital Anxiety and Depression Scale, the Self‐Administered Co‐morbidity Questionnaire and questions about other painful joints. Patients then completed the WOMAC Pain and Function Scales at one year postoperatively. Regression analysis was performed to determine if self‐efficacy was a significant predictor of outcome after TKR.ResultsOverall, 251 patients were recruited into this study, and one‐year questionnaire data were available for 220 patients. At one year postoperatively, 7% of patients reported severe pain in their replaced knee and 9% reported severe functional limitations. Self‐efficacy was found to be a significant preoperative predictor of functional ability, but not pain, at one year postoperatively, after controlling for age, gender, depression, anxiety, number of medical co‐morbidities, preoperative knee status and painful joints elsewhere. Significant predictors of postoperative pain were greater anxiety and higher pain severity. Other significant predictors of postoperative disability were greater anxiety, worse functional disability and a greater number of painful joints elsewhere.ConclusionsThe present study demonstrated that self‐efficacy is a significant preoperative predictor of patient‐reported functional ability at one year after TKR. Future research is needed to assess the impact of interventions for enhancing self‐efficacy on patient‐reported outcomes after TKR. Copyright © 2012 John Wiley & Sons, Ltd.
Decay‐accelerating factor 1 deficiency exacerbates Trypanosoma cruzi‐induced murine chronic myositis
Authors: Solana María E. , Ferrer María F. , Novoa María Mercedes , Song Wen‐Chao , GÓmez Ricardo M. 
Published: February 2012

AbstractIntroduction: Murine infection with Trypanosoma cruzi (Tc) has been used to study the role of T‐cells in the pathogenesis of human inflammatory idiopathic myositis. Absence of decay‐accelerating factor 1 (Daf1) has been shown to enhance murine T‐cell responses and autoimmunity. Methods: To determine whether Daf1 deficiency can exacerbate Tc‐induced myositis, C57BL/6 DAF+/+ and DAF−/− mice were inoculated with 5 × 104 trypomastigotes, and their morbidity, parasitemia, parasite burden, histopathology, and T‐cell expansion were studied in the acute and chronic stages. Results: DAF−/− mice had lower parasitemia and parasite burden but higher morbidity, muscle histopathology, and increased number of CD44+ (activated/memory phenotype) splenic CD4+ and CD8+ T‐cells. Conclusions: An enhanced CD8+ T‐cell immune‐specific response may explain the lower parasitemia and parasite burden levels and the increase in histopathological lesions. We propose that Tc‐inoculated DAF−/− mice are a useful model to study T‐cell mediated immunity in skeletal muscle tissues. Muscle Nerve 46: 582–587, 2012
Grip and Knee extension muscle strength reflect a common construct among adults
Authors: Bohannon Richard W. , Magasi Susan R. , Bubela Deborah J. , Wang Ying‐Chih , Gershon Richard C. 
Published: February 2012

AbstractIntroduction: Both grip and knee extension strength are often used to characterize overall limb muscle strength. We sought to determine if the measures actually reflect a common construct. Methods: The isometric grip and knee extension strength of 164 healthy men and women (range, 18–85 years) were measured bilaterally using standard procedures. Pearson correlations (r), Cronbach alpha, principal components analysis, and multiple regression/correlation were used to investigate the dimensionality of the measures. Results: Left and right grip forces and knee extension torques were highly correlated, internally consistent, and loaded on a single component. Gender and age explained the variance in both measures, but height added to the explanation of grip strength, whereas weight added to the explanation of knee extension strength. Conclusions: Among healthy adults, grip and knee extension strength reflect a common underlying construct. The measures, however, are affected differently by height and weight. Muscle Nerve 46: 555–558, 2012
Assessment of musculo‐articular and muscle stiffness in young and older men
Authors: Ditroilo Massimiliano , Cully Louise , Boreham Colin A.G. , De Vito Giuseppe 
Published: February 2012

AbstractIntroduction: The aim of this cross‐sectional study was to concurrently assess musculo‐articular stiffness (MAS) and muscle stiffness (MS) of the knee extensors in younger and older individuals. Methods: Fourteen young (22.1 ± 3.0 years old) and 12 older (65.4 ± 5.7 years old) men were tested for maximal voluntary contraction (MVC), rate of torque development (RTD), muscle thickness, MAS, and MS of knee extensors. Results: MVC, RTD, and muscle thickness were higher in the younger group (288.6 vs. 194.3 Nm, 1319.5 vs. 787.0 Nm s−1, 23.1 vs. 17.7 mm, respectively, P < 0.05). MAS normalized to the load supported (30% of MVC) was not different between groups (87.9 vs. 88.5 Nm−1kg−1), whereas the older group exhibited a higher level of normalized MS (23.2 vs. 18.6 Nm−1kg−1, P < 0.05). Conclusions: Determinants of MS have been highlighted along with their role in elevated MS. The unaltered level of MAS, which is functionally important in an aging population, might be achieved through a decrease in tendon stiffness. Muscle Nerve 46: 559–565, 2012