bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pariah Dog at the Chittagong University Campus: Home Range, Activity Pattern and Breeding
Authors: Ahsan Md Farid , Rajia Sultana 
Published: May 2024

Home range, activity pattern and breeding aspects of the pariah dog (Canis lupus familiaris Linnaeus, 1786) were studied at the Chittagong University Campus in Chittagong of Bangladesh during September 2012 to January 2013. The average home range encircled during daytime by a pack was 10,120 m2. The activity pattern of two dog packs was monitored through scan sampling methods at 15 minutes intervals. The dogs spent their diurnal time in resting (24.5%), sitting and basking (17.1%), standing (2.4%), moving and walking (16.2%), running (17.5%), sleeping (5.3%), feeding, foraging and drinking (6.3%), social behavior (2.9%) and territorial behavior (7.8%). Parturition occurred from September to January and the peak period was in November. The average litter size was 4.7 (range 1-12, n=10). and the overall survival rate of puppies was 2.6 per litter (i.e., 55.32%) up to January.
The Chittagong Univ. J. B. Sci. 8(1&2): 21-32, 2013
Latex Yield and Tree Growth in Kanchannagar Rubber Estate of Bangladesh - a Preliminary Study
Authors: Rahman M M , Hossain A T M Emdad , Alam M Z , Mahmud S 
Published: May 2024

Rubber (Hevea brasiliensis Muell. Arg.) is a perennial, hardy and moderately quick-growing tree of the tropical regions (Khisa 1991). An initiative of its cultivation was taken in Bangladesh during 1960-61 considering the multiple uses of the latex and its products including the utility of timber (Chowdhury 1974). In Bangladesh, experts recommended its cultivation in the hilly land, terrace mostly evaluating the land suitability class and within the limitation of climatic factors (Hasan 1983). Bangladesh Forest Industry Development Cooperation (BFIOC) sent a proposal to Bangladesh Forest Research Institute (BFRl), Chittagong to find out the causes of latex yield variation in the rubber station of Rangamatia and Doluchari of Kanchannagar Rubber Estate of Fatickchhari upazila of Chittagong District. Thus, a preliminary study was conducted on these rubber gardens during 2007-08. The clones in those estates are RRlM-600; PBIG/GG, GT-1 and PB-235, which were planted during different planting years (BFIDC 1993). Management of the Doluchari garden was comparatively better than Rangamatia. Total number of trees in Doluchari and Rangamatia is 29850 and .36149 respectively. Two (2004-06) years latex yield records showed that average productions were 3.16 kg/tre/yr for Doluchari and 1.86 kg/tree/yr in Rangamatia. The latex yield variation may be due to different factors viz: site quality, management efficiency, age group of clones, etc. (Anon,1989).
The Chittagong Univ. J. B. Sci. 8(1&2): 33-36, 2013
Penanaman Nilai-Nilai Kristen Berdasarkan Ulangan 6:7 bagi Pertumbuhan Manusia Rohani Anak
Authors: Samly Deny , Saptono Yohanes Joko 
Published: May 2024

Children's spiritual growth is significant for parents to pay attention to, and it is a task given by God to parents to become the main teacher for children's spiritual growth. This article explains the cultivation of Christian values based on Deuteronomy 6:7 for children's spiritual growth. Deuteronomy 6:7 states that God commands that parents carry this responsibility wholeheartedly to teach the diligent cultivation of Christian values, namely to give them in upbringing to children repeatedly. The thing instilled in the child is the most important and foremost to love God with all his heart, as stated in the previous verse (Deuteronomy 6:4-9). The cultivation of values to live produces the fruit of the spirit in their lives, and it is certainly the role of the work of the Holy Spirit that guides their lives. Every instillation of value given by parents to children that is carried out repeatedly will the child remember and apply it in their lives. Through this, they will also experience spiritual growth that loves God and makes God's words the foundation and principle of their lives. AbstrakPetumbuhan rohani anak sangat penting untuk diperhatikan orang tua dan adalah sebuag tugas yang diberikan oleh Tuhan kepada orang tua untuk menjadi guru utama bagi pertumbuhan kerohanian anak. Penulisan artikel ini bertujuan untuk menjelaskan tentang penanaman nilai-nilai Kristen yang berdasarkan pada Ulangan 6:7 bagi pertumbuhan rohani anak. Ulangan 6:7 menyatakan bahwa Tuhan memerintahkan agar orang tua membawa tanggung jawab ini dengan sepenuh hati untuk mengajarkan penanaman nilai Kristen dengan tekun yaitu memberikannya dalam didikan kepada anak secara berulang-ulang. Hal yang ditanamkan kepada anak yang terpenting dan terutama untuk mengasihi Allah dengan segenap hati seperti yang dinyatakan dalam ayat sebelum-sebelumnya (Ul 6:4-9). Penanaman nilai-nilai melainkan untuk hidup menghasilkan buah roh dalam hidup mereka dan sudah pasti adalah adanya peran karya dari Roh Kudus yang membimbing hidup mereka. Setiap penanaman nilai yang diberikan oleh orang tua kepada anak yang dilakukan secara berulang-ulang akan anak ingat dan mengaplikasikannya dalam kehidupan mereka. Melalui hal ini juga mereka akan mengalami pertumbuhan rohani yang mengasihi Tuhan dan menjadikan firman Tuhan sebagai pondasi serta prinsip dalam kehidulpan mereka.
Pola Pendidikan Agama Kristen yang Humanis dalam Pembentukan Perilaku Anak Usia 6-12 Tahun Melalui Persekutuan Doa Efata Jemaat GMIT Betlehem Oesapa Barat, Kota Kupang
Authors: Selan Yeson A. , Natonis Harun Y. , Tari Ezra 
Published: May 2024

Persekutuan Doa Efata Jemaat GMIT Oesapa Barat Kota Kupang memiliki permasalahan yang dihadapi yakni: pertama, belum adanya Pendidikan Agama Kristen Yang Humanis. Kedua, pembentukan perilaku anak dalam ibadah. Kondisi perilaku anak-anak di kalangan persekutuan doa tidak tertib dalam ibadah Persekutuan doa. Kondisi seperti ini mendatangkan sorotan dari oknum-oknum tertentu menyangkut perilaku anak-anak dalam persekutuan doa tersebut. Metode yang dipakai adalah kualitatif deskriptif. Penting untuk diingat bahwa setiap anak adalah individu yang unik, dan pendekatan pendidikan agama Kristen yang humanis harus memerhatikan kebutuhan, minat, dan perkembangan anak secara holistik. Pengurus membuat grup WhatsApp agar pengajar membagikan video-video yang berkaitan dengan Pendidikan Humanis lewat grup tersebut. Pengajar mengarahkan anak-anak dengan lemah lembut, bukan dengan kekerasan.
Dreams
Authors: Wadiwel Dinesh Joseph 
Published: May 2024

This concluding Chapter explores the implications of this book for thinking about how we move forward, focusing on framing, tactics and goals. Exploring Marx’s notes on communism in Critique of the Gotha Program, the Chapter asks how animals are placed with respect to the means of production, and what liberation might mean. Human worker control of the means of production does not necessarily entail freedom for animals in their roles as raw materials, labour or their eventual transformation into the means of subsistence. This is because, quite simply, food animals under capitalism are the means of production. However, a post-anthropocentric focus on a reduction in necessary labour time offers a useful trajectory for imagining a world where animals are freed of the necessity of having to labour in order to produce surplus or become a means of subsistence for humans. Perhaps this route provides one way to overcome the structural divisions that mark the difference between the human worker and the food animal under capitalism.
Understanding mitochondrial potassium channels: 33 years after discovery
Authors: Szewczyk Adam 
Published: May 2024

Mitochondrial investigations have extended beyond their traditional functions, covering areas such as ATP synthesis and metabolism. Mitochondria are now implicated in new functional areas such as cytoprotection, cellular senescence, tumor function and inflammation. The basis of these new areas still relies on fundamental biochemical/biophysical mitochondrial functions such as synthesis of reactive oxygen species, mitochondrial membrane potential, and the integrity of the inner mitochondrial membrane i.e., the passage of various molecules through the mitochondrial membranes. In this view transport of potassium cations, known as the potassium cycle, plays an important role. It is believed that K+ influx is mediated by various potassium channels present in the inner mitochondrial membrane. In this article, we present an overview of the key findings and characteristics of mitochondrial potassium channels derived from research of many groups conducted over the past 33 years. We propose a list of six fundamental observations and most important ideas dealing with mitochondrial potassium channels. We also discuss the contemporary challenges and future prospects associated with research on mitochondrial potassium channels.
The association between telomere length and blood lipids: a bidirectional two-sample Mendelian randomization study
Authors: Yang Shengjie , Wang Xinyue , Li Yujuan , Zhou Lijun , Guo Gang , Wu Min 
Published: May 2024

BackgroundObservational studies suggest an association between telomere length (TL) and blood lipid (BL) levels. Nevertheless, the causal connections between these two traits remain unclear. We aimed to elucidate whether genetically predicted TL is associated with BL levels via Mendelian randomization (MR) and vice versa.MethodsWe obtained genetic instruments associated with TL, triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), apolipoprotein A-1 (ApoA-1) and apolipoprotein B (ApoB) from large-scale genome-wide association studies (GWASs). The causal relationships between TL and BL were investigated via bidirectional MR, multivariable MR and mediation analysis methods. The inverse variance weighted (IVW) method was employed as the principal methodology, complemented by several other estimators to enhance the robustness of the analysis.ResultsIn the forward MR analyses, we identified significant positive correlation between genetically predicted TL and the levels of TG (β=0.04, 95% confidence interval [CI]: 0.01 to 0.06, p = 0.003). In the reverse MR analysis, TG (β=0.02, 95% CI: 0.01 to 0.03, p = 0.004), LDL-C (β=0.03, 95% CI: 0.01 to 0.04, p = 0.001) and ApoB (β=0.03, 95% CI: 0.01 to 0.04, p = 9.71×10–5) were significantly positively associated with TL, although this relationship was not observed in the multivariate MR analysis. The mediation analysis via two-step MR showed no significant mediation effects acting through obesity-related phenotypes in analysis of TL with TG, while the effect of LDL-C on TL was partially mediated by body mass index (BMI) in the reverse direction, with mediated proportion of 12.83% (95% CI: 0.62% to 25.04%).ConclusionsOur study indicated that longer TL were associated with higher TG levels, while conversely, higher TG, LDL-C, and ApoB levels predicted longer TL, with BMI partially mediating these effects. Our findings present valuable insights into the development of preventive strategies and interventions that specifically target TL-related aging and age-related diseases.
Identification of potential biomarkers and pathways for asthenozoospermia by bioinformatics analysis and experiments
Authors: Lu Hui , Zhao Liqiang , Wang Anguo , Ruan Hailing , Chen Xiaoyan , Li Yejuan , Hu Jiajia , Lu Weiying , Xiao Meifang 
Published: May 2024

BackgroundAsthenozoospermia, a type of male infertility, is primarily caused by dysfunctional sperm mitochondria. Despite previous bioinformatics analysis identifying potential key lncRNAs, miRNAs, hub genes, and pathways associated with asthenospermia, there is still a need to explore additional molecular mechanisms and potential biomarkers for this condition.MethodsWe integrated data from Gene Expression Omnibus (GEO) (GSE22331, GSE34514, and GSE160749) and performed bioinformatics analysis to identify differentially expressed genes (DEGs) between normozoospermia and asthenozoospermia. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to gain insights into biological processes and signaling pathways. Weighted Gene Co-expression Network Analysis (WGCNA) identified gene modules associated with asthenozoospermia. Expression levels of key genes were assessed using datasets and experimental data. Gene Set Enrichment Analysis (GSEA) and correlation analysis identified pathways associated with the hub gene and explore the relationship between the ZNF764 and COQ9 and mitochondrial autophagy-related genes. Competitive endogenous RNA (ceRNA) networks were constructed, and in vitro experiments using exosome samples were conducted to validate this finding.ResultsCOQ9 was identified as a marker gene in asthenozoospermia, involved in autophagy, ATP-dependent chromatin remodeling, endocytosis, and cell cycle, etc. The ceRNA regulatory network (LINC00893/miR-125a-5p/COQ9) was constructed, and PCR demonstrated that LINC00893 and COQ9 were downregulated in asthenozoospermia, while miR-125a-5p and m6A methylation level of LINC00893 were upregulated in asthenozoospermia compared to normozoospermic individuals.ConclusionThe ceRNA regulatory network (LINC00893/miR-125a-5p/COQ9) likely plays a crucial role in the mechanism of asthenozoospermia. However, further functional experiments are needed to fully understand its significance.
Immunization with PfGBP130 generates antibodies that inhibit RBC invasion by P. falciparum parasites
Authors: Johnson Yannick , Shakri Ahmad Rushdi , Pond-Tor Sunthorn , Jnawali Anup , Najrana Tanbir , Wu Haiwei , Badhai Jhasketan , Alameh Mohamad-Gabriel , Weissman Drew , Kabyemela Edward , Duffy Patrick , Fried Michal , Kurtis Jonathan , Raj Dipak Kumar 
Published: May 2024

BackgroundDespite decades of effort, Plasmodium falciparum malaria remains a leading killer of children. The absence of a highly effective vaccine and the emergence of parasites resistant to both diagnosis as well as treatment hamper effective public health interventions.Methods and resultsTo discover new vaccine candidates, we used our whole proteome differential screening method and identified PfGBP130 as a parasite protein uniquely recognized by antibodies from children who had developed resistance to P. falciparum infection but not from those who remained susceptible. We formulated PfGBP130 as lipid encapsulated mRNA, DNA plasmid, and recombinant protein-based immunogens and evaluated the efficacy of murine polyclonal anti-PfGBP130 antisera to inhibit parasite growth in vitro. Immunization of mice with PfGBP130-A (aa 111–374), the region identified in our differential screen, formulated as a DNA plasmid or lipid encapsulated mRNA, but not as a recombinant protein, induced antibodies that inhibited RBC invasion in vitro. mRNA encoding the full ectodomain of PfGBP130 (aa 89–824) also generated parasite growth-inhibitory antibodies.ConclusionWe are currently advancing PfGBP130-A formulated as a lipid-encapsulated mRNA for efficacy evaluation in non-human primates.
The role of donor hypertension and angiotensin II in the occurrence of early pancreas allograft thrombosis
Authors: Masset Christophe , Branchereau Julien , Buron Fanny , Karam Georges , Rabeyrin Maud , Renaudin Karine , Le Borgne Florent , Badet Lionel , Matillon Xavier , Legendre Christophe , Glotz Denis , Antoine Corinne , Giral Magali , Dantal Jacques , Cantarovich Diego ,   DIVAT Consortium
Published: May 2024

BackgroundAbout 10–20% of pancreas allografts are still lost in the early postoperative period despite the identification of numerous detrimental risk factors that correlate with graft thrombosis.MethodsWe conducted a multicenter study including 899 pancreas transplant recipients between 2000 and 2018. Early pancreas failure due to complete thrombosis, long-term pancreas, kidney and patient survivals were analyzed and adjusted to donor, recipient and perioperative variables using a multivariate cause-specific Cox model stratified to transplant centers.ResultsPancreas from donors with history of hypertension (6.7%), as well as with high body mass index (BMI), were independently associated with an increased risk of pancreas failure within the first 30 post-operative days (respectively, HR= 2.57, 95% CI from 1.35 to 4.89 and HR= 1.11, 95% CI from 1.04 to 1.19). Interaction term between hypertension and BMI was negative. Donor hypertension also impacted long-term pancreas survival (HR= 1.88, 95% CI from 1.13 to 3.12). However, when pancreas survival was calculated after the postoperative day 30, donor hypertension was no longer a significant risk factor (HR= 1.22, 95% CI from 0.47 to 3.15). A lower pancreas survival was observed in patients receiving a pancreas from a hypertensive donor without RAAS (Renin Angiotensin Aldosterone System) blockers compared to others (50% vs 14%, p &lt; 0.001). Pancreas survival was similar among non-hypertensive donors and hypertensive ones under RAAS blockers.ConclusionDonor hypertension was a significant and independent risk factor of pancreas failure. The well-known pathogenic role of renin-angiotensin-aldosterone system seems to be involved in the genesis of this immediate graft failure.
Mangrove sediments-associated bacterium (Bacillus sp. SW7) with multiple plant growth-promoting traits promotes the growth of tomato (Solanum Lycopersicum)
Authors: Afridi Mahideen , Sadaiappan Balamurugan , Nassar Amna Saood , Mahmoudi Henda , Khan Munawwar Ali , Mundra Sunil 
Published: May 2024

Global food production intensification presents a major hurdle to ensuring food security amidst a growing world population. Widespread use of chemical fertilizers in recent decades has risked soil fertility, compounded by the challenges posed by climate change, particularly in arid regions. To address these issues, adopting plant growth-promoting (PGP) bacteria stands out as a promising solution, offering multifaceted benefits to arid agroecosystems. We isolated a bacterial strain, SW7, from mangrove sediment, characterised the entire genome followed by phylogenetic analyses, and evaluated its in-vitro PGP activity. Subsequently, we examined its impact on tomato seed germination and plant growth. The strain SW7 exhibited growth on 11% NaCl, survival at 50°C, and possessed multiple PGP traits such as significant increase in seed germination rate (60.60 ± 38.85%), phosphate (83.3 g L−1) and potassium (39.6 g L−1) solubilization and produced indole acetic acid (3.60 ppm). Additionally, strain SW7 tested positive for ammonia, catalase, and oxidase enzyme production. The strain SW7 genome consists of 5.1 MB with 35.18% G+C content. Through genome-based phylogenetic and orthoANI analyses, the strain was identified as a novel Bacillus species, designated herein as Bacillus sp. SW7. In an eight-week shade-house experiment, inoculation of strain SW7 improved, leaf number, leaf density, leaf area index and mass water of tomatoes. Additional parameters, like chlorophyll a, chlorophyll b and carotenoids were not affected in SW7-inoculated tomatoes. In conclusion, Bacillus sp. SW7 exhibits multiple PGP traits and an adaptive capacity to high temperature and salinity, positioning it as a potential candidate for elevating the productivity of arid agroecosystems.
Efficiency of polymyxin B treatment against nosocomial infection: a systematic review and meta-analysis
Authors: Peng Liyuan , Zhang Zhongheng , Qi Xueyan , Zhong Yanjun , Sun Tongwen , Chen Lvlin , Zhu Junchen , Lv Xiangui , Ma Penglin 
Published: May 2024

BackgroundSome cohort studies have explored the effects and safety of polymyxin B (PMB) in comparison to other antibiotics for the treatment of nosocomial infections, yielding inconsistent results. This systematic review aims to explore the effectiveness and safety of PMB and compared it with other antibiotics.MethodsA systematic literature search was conducted in PubMed, Embase, the Cochrane Library, and Web of Science, searching specific terms to identify quantitative cohort studies or RCTs that compared the effects of PMB with other antibiotics in terms of their efficacy and safety. The Newcastle–Ottawa Scale (NOS) was conducted to evaluate the risk of bias of observational studies. Odds ratios with 95% confidence intervals were used for outcome assessment. We evaluated heterogeneity using the I2 test.ResultsA total of 22 observational trials were included in the analysis. The PMB group had a higher mortality rate compared to the control group (odds ratio: 1.84, 95% CI: 1.36–2.50, p&lt;0.00001, I2 = 73%). while, the ceftazidime-avibactam group demonstrated a distinct advantage with lower mortality rates, despite still exhibiting high heterogeneity (odds ratio 2.73, 95% confidence interval 1.59–4.69; p = 0.0003; I2 = 53%). Additionally, the PMB group had a lower nephrotoxicity rate compared to the colistin group but exhibited high heterogeneity in the results (odds ratio 0.58, 95% CI 0.36–0.93; p = 0.02; I2 = 73%).ConclusionIn patients with nosocomial infections, PMB is not superior to other antibiotics in terms of mortality, specifically when compared to ceftazidime-avibactam. However, PMB demonstrated an advantage in terms of nephrotoxicity compared to colistin.
The characteristics of brain atrophy prior to the onset of Alzheimer’s disease: a longitudinal study
Authors: Hu Ying , Zhu Ting , Zhang Wei 
Published: May 2024

ObjectiveWe aimed to use the onset time of Alzheimer’s disease (AD) as the reference time to longitudinally investigate the atrophic characteristics of brain structures prior to the onset of AD.Materials and methodsA total of 328 participants from the ADNI database with clear onset of AD and structural imaging data were included in our study. The time before the onset of AD (abbreviated as BAD) was calculated. We investigated the longitudinal brain changes in 97 regions using multivariate linear mixed effects regression models.ResultsThe average BAD was −28.15 months, with a range from −156 to 0 months. The 54 brain regions showed significant atrophy prior to the onset of AD, and these regions were mainly distributed in the frontal and temporal lobes. The parietal and occipital lobe exhibited relatively less atrophy than the other brain lobes. Sex, age, and magnetic field strength had greater direct impacts on structural indicators than APOE genotype and education. The analysis of interaction effects revealed that the APOE ε4 mutation carriers exhibited more severe structural changes in specific brain regions as the BAD increased. However, sex, age, and education had minimal regulatory influence on the structural changes associated with BAD.ConclusionLongitudinal analysis, with the onset time point of AD as the reference, can accurately describe the features of structural changes preceding the onset of AD and provide a comprehensive understanding of AD development.
Care dependency in older stroke patients with comorbidities: a latent profile analysis
Authors: Lin Qinger , Dong Xiaohang , Huang Tianrong , Zhou Hongzhen 
Published: May 2024

ObjectivesTo explore latent profiles of care dependency in older stroke patients with comorbidities and to analyze the factors influencing different latent profiles.MethodsA total of 312 older ischemic stroke patients with comorbidities were included in the analysis. Latent Profile Analysis (LPA) was used to classify the participants into potential subgroups with different types of care dependency. The influencing factors of the classification of care dependency subgroups were determined using multivariate Logistic regression analysis.ResultsThe care dependency score of older ischemic stroke patients with comorbidities was (51.35 ± 13.19), and the patients could be classified into 3 profiles, namely Universal dependency (24.0%), Moderate activity-social-learning dependency (28.0%), and Mild activity-social-learning dependency (48.0%); caregiver, BI at admission, and functional impairments were independent factors influencing care dependency (P &lt; 0.05).ConclusionThere are three latent profiles of care dependency in older ischemic stroke patients with comorbidities. According to the characteristics of various populations, medical staff are able to implement specific interventions to lower the level of dependency and further improve the quality of life of patients.