bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical features, health‐related quality of life, and adult voice in juvenile‐onset recurrent respiratory papillomatosis
Authors: Ilmarinen Taru , Nissilä Heljä , Rihkanen Heikki , Roine Risto P , Pietarinen‐Runtti Petra , Pitkäranta Anne , Aaltonen Leena‐Maija 
Published: March 2011

AbstractObjectives/Hypothesis:To determine clinical features, health‐related quality of life, and adult voice in patients with a history of juvenile‐onset recurrent respiratory papillomatosis (JORRP).Study Design:Case‐control study.Methods:All 32 patients with JORRP treated at Helsinki University Hospital between 1975 and 1994 were invited to an outpatient visit in spring 2008, and 18 of them (56%) entered the study. Each patient had an age‐ and gender‐matched control subject with similar smoking habits. Videolaryngostroboscopy was performed and voice quality determined by acoustic and perceptual analysis. Voice‐quality characteristics of the whole patient group and the recurrence‐free patients were examined separately. Subjective voice‐related disability was studied with voice handicap index (VHI) and health‐related quality of life with a 15D questionnaire.Results:Acoustic analysis showed that patients had statistically significantly higher values in percent jitter, percent shimmer, and noise‐to‐harmonics ratio. Perceptual analysis indicated higher scores for patients in overall grade, roughness, breathiness, and strain. Acoustic and perceptual values for recurrence‐free patients (n = 14) were also significantly higher than those for their matched paired controls. No statistically significant differences emerged for handicap related to voice or to health‐related quality of life. Four study patients (22%) had undergone tracheotomy, indicating severity of juvenile‐onset disease.Conclusions:JORRP is a risk factor for permanent laryngeal pathology and voice‐disturbances in adulthood. Laryngoscope, 2011
The Use of Poly(L‐lactide‐co‐caprolactone) as a Scaffold for Adipose Stem Cells in Bone Tissue Engineering: Application in a Spinal Fusion Model
Authors: Vergroesen Pieter‐Paul A. , Kroeze Robert‐Jan , Helder Marco N. , Smit Theodoor H. 
Published: March 2011

AbstractSince the early 1990s, tissue engineering has been heralded as a strategy that may solve problems associated with bone grafting procedures. The original concept of growing bone in the laboratory, however, has proven illusive due to biological, logistic, and regulatory problems. Fat‐derived stem cells and synthetic polymers open new, more practicable routes for bone tissue engineering. In this paper, we highlight the potential of poly(L‐lactide‐co‐caprolactone) (PLCL) to serve as a radiolucent scaffold in bone tissue engineering. It appears that PLCL quickly and preferentially binds adipose stem cells (ASCs), which proliferate rapidly and eventually differentiate into the osteogenic phenotype. An in vivo spinal fusion study in a goat model provides a preclinical proof‐of‐concept for a one‐step surgical procedure with ASCs in bone tissue engineering. magnified image
Construction of a Hyperbranched Supramolecular Polymer as a Bifunctional Antioxidative Enzyme Model
Authors: Yu Shuangjiang , Zhang Wei , Zhu Junyan , Yin Yanzhen , Jin Haiyan , Zhou Lipeng , Luo Quan , Xu Jiayun , Liu Junqiu 
Published: March 2011

AbstractA HBSP has been designed as a novel bifunctional enzyme model with SOD and GPx activity by host/guest‐directed self‐assembly of MnTPyP‐M‐Ad and 6‐Te‐diCD. The structure of the host/guest complex was elucidated by 1H NMR spectra, and the HBSP was characterized by SEM, DLS and measurement of catalytic properties. In the bifunctional enzyme model, the Mn(III) porphyrins act as efficient SOD active sites and the tellurol moieties endow GPx activity. The SOD‐like activity (IC50) of this new supramolecular catalyst was found to be 1.05 × 10−7 M, which corresponds to 2.82% of the activity of the native SOD enzyme. Besides this, the hyperbranched supramolecular polymer also shows a higher GPx activity (ν0 = 21.7 × 10−6 M · min−1) than other supramolecular enzyme models. magnified image
Azide‐based Cross‐Linking of Polymers of Intrinsic Microporosity (PIMs) for Condensable Gas Separation
Authors: Du Naiying , Cin Mauro M. Dal‐ , Pinnau Ingo , Nicalek Andrzej , Robertson Gilles P. , Guiver Michael D. 
Published: March 2011

AbstractCross‐linked polymers of intrinsic microporosity (PIM)s for gas separation membranes, were prepared by a nitrene reaction from a representative PIM in the presence of two different diazide cross‐linkers. The reaction temperature was optimized using TGA. The homogenous membranes were cast from THF solutions of different ratios of PIM to azides. The resulting cross‐linked structures of the PIMs membranes were formed at 175 °C after 7.5 h and confirmed by TGA, XPS, FT‐IR spectroscopy and gel content analysis. These resulting cross‐linked polymeric membranes showed excellent gas separation performance and can be used for O2/N2 and CO2/N2 gas pairs, as well as for condensable gases, such as CO2/CH4, propylene/propane separation. Most importantly, and differently from typical gas separation membranes derived from glassy polymers, the crosslinked PIMs showed no obvious CO2 plasticization up to 20 atm pressure of pure CO2 and CO2/CH4 mixtures. magnified image
Electrochemical Fluorescence Switching from a Patternable Poly(1,3,4‐oxadiazole) Thin Film
Authors: Seo Seogjae , Kim Yuna , You Jungmok , Sarwade Bhimrao D. , Wadgaonkar Prakash P. , Menon Shamal K. , More Arvind S. , Kim Eunkyoung 
Published: March 2011

AbstractA highly soluble poly(1,3,4‐oxadiazole) (POD) substituted with long alkyl chains was examined for electrochemical fluorescence switching. The high solubility of the polymers enabled a simple fabrication of an electrochemical cell, which showed reversible fluorescence switching between dark (n‐doping) and bright (neutral) states with a maximum on/off ratio of 2.5 and a cyclability longer than 1 000 cycles. Photochemical cleavage of the oxadiazole in POD allowed photo‐patterning of the POD film upon exposure to UV source. The patterned POD films displayed patterned image reversibly under a step potential of +1.8/−1.8 V. magnified image
Application of Alkyne‐TCNQ Addition Reaction to Polymerization
Authors: Washino Yusuke , Michinobu Tsuyoshi 
Published: March 2011

AbstractThe polymerization using a high‐yielding addition reaction between electron‐rich alkynes and 7,7,8,8‐tetracyanoquinodimethane (TCNQ) derivatives is described. The bifunctional monomer containing two TCNQ moieties and the counter comonomer bearing two dialkylaniline (DAA)‐substituted alkynes are reacted in 1,2‐dichloroethane under mild heating conditions. At the high monomer concentrations, high molecular weight linear polymers are obtained, while the reaction at the low monomer concentrations produces a significant amount of the cyclic compounds. A clear relationship between the monomer concentration and the cyclic compound amount is demonstrated. The obtained polymers feature a sufficient thermal stability with the decomposition temperature exceeding 300 °C as well as strong charge‐transfer (CT) bands and redox activities ascribed to the produced donor–acceptor moieties. These features are also used to optimize the polymerization conditions and to estimate the chemical structures. magnified image
Studies of cyclization reactions of linear cumulenes and heterocumulenes using the neutralization–reionization procedure and/or ab initio calculations
Authors: Wang Tianfang , Bowie John H. 
Published: March 2011

AbstractA number of linear cumulenes and heterocumulenes have been made by charge stripping of anions of known bond connectivity in the source of a mass spectrometer. Some of these reactive molecules have been identified in interstellar molecular clouds. The structures of these neutrals may be investigated by reionization to a decomposing positive ion [the neutralization–reionization technique (−NR+)], and/or by ab initio calculations. Energized linear cumulenes and heterocumulenes may undergo cyclization to form stable cyclic isomers. To cite a selection of the examples described in this review: (i) four‐atom systems CCCC and some heterocumulenes CCCX (X = B, N, Al, Si, P) involve the formation of stable four‐membered ring rhombic (also called kite and fan) structures. One of the cyclic molecules, cyclo‐C3Si, has been detected in interstellar molecular clouds, (ii) five‐atom cumulene and heterocumulene systems are more complex. Linear CCCCC rearranges the carbon skeleton by forming a C substituted rhomboid system, CCCCO forms a three‐membered cyclic isomer, while nitrogen containing five‐atom cumulenes effect nitrile to isonitrile interconversion via three‐centered cyclized intermediates, and (iii) CCCCCC and CCCCBO cyclize to give unique six‐membered ring systems. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:1225–1241, 2011
From reticulate doped to nanostructural polymeric systems
Authors: Kryszewski Marian 
Published: March 2011

AbstractPreparation of very small or nanoscopic crystallites applying wet techniques and their properties are discussed. It is shown that controlled evaporation of the solvent from the solution containing low molecular weight CT complexes and polymers leads to formation of continuous networks of microcrystallites, which are responsible for the magnetic, optical and particularly electrical properties of the obtained systems (isotropic, surface or anisotropic conductivity). Using a variation of this method one can obtain very small crystals of CT complex salts or other substances showing nonlinear optical properties. Application of the so‐called “droplet technique” results in formation of the emulsion of the crystallizable compounds. The crystallization in small droplets leads to very small crystals which show, because of their nanoscopic size, different properties than bigger single crystals of these compounds.
Functional characterization of peroxisome proliferator‐activated receptor‐β/δ expression in colon cancer
Authors: Foreman Jennifer E. , Chang Wen‐Chi L. , Palkar Prajakta S. , Zhu Bokai , Borland Michael G. , Williams Jennie L. , Kramer Lance R. , Clapper Margie L. , Gonzalez Frank J. , Peters Jeffrey M. 
Published: March 2011

AbstractThis study critically examined the role of PPARβ/δ in colon cancer models. Expression of PPARβ/δ mRNA and protein was lower and expression of CYCLIN D1 protein higher in human colon adenocarcinomas compared to matched non‐transformed tissue. Similar results were observed in colon tumors from Apc+/Min‐FCCC mice compared to control tissue. Dietary administration of sulindac to Apc+/Min‐FCCC mice had no influence on expression of PPARβ/δ in normal colon tissue or colon tumors. Cleaved poly (ADP‐ribose) polymerase (PARP) was either increased or unchanged, while expression of 14‐3‐3ε was not influenced in human colon cancer cell lines cultured with the PPARβ/δ ligand GW0742 under conditions known to increase apoptosis. While DLD1 cells exhibited fewer early apoptotic cells after ligand activation of PPARβ/δ following treatment with hydrogen peroxide, this change was associated with an increase in late apoptotic/necrotic cells, but not an increase in viable cells. Stable over‐expression of PPARβ/δ in human colon cancer cell lines enhanced ligand activation of PPARβ/δ and inhibition of clonogenicity in HT29 cells. These studies are the most quantitative to date to demonstrate that expression of PPARβ/δ is lower in human and Apc+/Min‐FCCC mouse colon tumors than in corresponding normal tissue, consistent with the finding that increasing expression and activation of PPARβ/δ in human colon cancer cell lines inhibits clonogenicity. Because ligand‐induced attenuation of early apoptosis can be associated with more late, apoptotic/necrotic cells, but not more viable cells, these studies illustrate why more comprehensive analysis of PPARβ/δ‐dependent modulation of apoptosis is required in the future. © 2011 Wiley Periodicals, Inc.
Role of androgen receptor and associated lysine‐demethylase coregulators, LSD1 and JMJD2A, in localized and advanced human bladder cancer
Authors: Kauffman Eric C. , Robinson Brian D. , Downes Martin J. , Powell Leagh G. , Lee Ming Ming , Scherr Douglas S. , Gudas Lorraine J. , Mongan Nigel P. 
Published: March 2011

AbstractBladder cancer is approximately three times more common in men as compared to women. We and others have previously investigated the contribution of androgens and the androgen receptor (AR) to bladder cancer. JMJD2A and LSD1 are recently discovered AR coregulator proteins that mediate AR‐dependent transcription via recently described histone lysine‐demethylation (KDM) mechanisms. We used immunohistochemistry to examine JMJD2A, LSD1, and AR expression in 72 radical cystectomy specimens, resulting in evaluation of 129 tissue samples (59 urothelial carcinoma, 70 benign). We tested levels of these proteins for statistical association with clinicopathologic variables and patient survival. Expression of these markers was also assessed in human bladder cancer cell lines. The effects of pharmacological inhibition of LSD1 on the proliferation of these bladder cancer cells was determined. JMJD2A and AR levels were significantly lower in malignant versus benign urothelium, while increased LSD1 levels were observed in malignant urothelium relative to benign. A significant reduction in all three proteins occurred with cancer stage progression, including muscle invasion (JMJD2A/LSD1/AR), extravesical extension (JMJD2A/LSD1), and lymph node metastasis (JMJD2A/AR). Lower JMJD2A intensity correlated with additional poor prognostic features, including lymphovascular invasion, concomitant carcinoma in situ and tobacco usage, and predicted significantly worse overall survival. Pharmacological inhibition of LSD1 suppressed bladder cancer cell proliferation and androgen‐induced transcription. Our results support a novel role for the AR–KDM complex in bladder cancer initiation and progression, identify JMJD2A as a promising prognostic biomarker, and demonstrate targeting of the KDM activity as an effective potential approach for bladder cancer growth inhibition. © 2011 Wiley Periodicals, Inc.
Effect of polymorphisms of IL‐1β and TNF‐α genes on CpG island hyper methylation (CIHM) in the nonneoplastic gastric mucosa
Authors: Tahara Tomomitsu , Shibata Tomoyuki , Nakamura Masakatsu , Yamashita Hiromi , Yoshioka Daisuke , Okubo Masaaki , Yonemura Joh , Kamiya Yoshio , Ishizuka Takamitsu , Hirata Ichiro , Arisawa Tomiyasu 
Published: March 2011

AbstractCpG island hyper methylation (CIHM) is one of the major events in gastric carcinogenesis. To evaluate the influence of host genetic factors in CIHM related carcinogenesis, we investigated the association between common polymorphisms in IL‐1β and TNF‐α genes, with CIHM status in the nonneoplastic gastric mucosa. Polymorphisms in the IL‐1β gene (‐31T>C and ‐511C>T) and the TNF‐α gene (‐857C>T) were genotyped in 385 cancer‐free subjects. CIHM of four candidate genes: p16 (INK4a), p14 (ARF), E‐cadherin (CDH1), and death‐associated protein kinase (DAP‐kinase), were determined by methylation‐specific‐polymerase chain reaction (MSP). CIHM high was defined as two or more CpG islands methylated. CIHM of all four genes and CIHM high were significantly associated with Helicobacter pylori infection status. In over all, significant marginal association was found between IL‐1β‐511 TT genotype and reduced susceptibility to CIHM of DAP‐kinase (adjusted OR = 0.48, 95% CI = 0.29–0.78) and CIHM high (adjusted OR = 0.53, 95% CI = 0.32–0.86). This association was more enhanced in subjects 65 yr or younger age. We also found positive association between TNF‐α‐857T carrier and increased susceptibility to CIHM of CDH (adjusted OR = 1.78, 95% CI = 1.01–3.16), and CIHM high (adjusted OR = 1.86, 95% CI = 1.04–3.33) in the same generation. The mean number of CIHM was lower in subjects with IL‐1β‐511TT genotype, while the mean number was higher in subjects with TNF‐α‐857 T carrier especially in subjects 65 yr and younger patients. IL‐1β‐511 TT genotype is associated with reduced susceptibility to CIHM especially in younger generation. Furthermore, the TNF‐α‐857T carrier is associated with increased susceptibility of CIHM in the same generation. © 2011 Wiley Periodicals, Inc.
5′‐Nitro‐indirubinoxime inhibits epidermal growth factor‐ and phorbol ester‐induced AP‐1 activity and cell transformation through inhibition of phosphorylation of Pin1
Authors: Khanal Prem , Choi Hoo‐Kyun , Namgoong Gwang Mo , Ahn Sang‐Gun , Yoon Jung‐Hoon , Sohn Honglae , Choi Hong Seok 
Published: March 2011

Abstract5′‐Nitro‐indirubinoxime (5′‐NIO), a derivative of indirubin, exhibits anti‐cancer activity in a variety of human cancer cells. However, the underlying molecular mechanisms and molecular target(s) of the chemopreventive activities of 5′‐NIO remain unknown. Here, we report that 5′‐NIO inhibited the epidermal growth factor (EGF) or 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)‐induced neoplastic cell transformation of JB6 Cl41 mouse skin epidermal cells without any cytotoxic effects. Western blot analysis revealed that 5'‐NIO inhibited activities of Raf‐1 (S338), MEK1/2, ERK1/2, JNK, and c‐Jun induced by EGF or TPA, respectively, whereas it did not affect autophosphorylation of epidermal growth factor receptor (EGFR) induced by EGF or TPA. In addition, 5′‐NIO exerted strong inhibitory effects on the EGF‐ or TPA‐induced c‐fos or c‐jun transcriptional activity, and thereby inhibited the associated activator protein‐1 (AP‐1) transactivation activity induced by EGF or TPA. Importantly, 5'‐NIO inhibited Pin1 phosphorylation at serine 16 induced by EGF or TPA, respectively, resulted in the inhibition of interaction between Pin1 and Raf‐1. Immunoprecipitation/immunoblot analysis revealed that 5'‐NIO bound with Pin1. Together, these findings suggest that 5'‐NIO might act as an anticarcinogene in EGF‐ or TPA‐induced carcinogenesis through the inhibition of interaction between Pin1 and Raf‐1. © 2011 Wiley Periodicals, Inc.
β‐Escin sodium inhibits inducible nitric oxide synthase expression via downregulation of the JAK/STAT pathway in A549 cells
Authors: Ji Deng Bo , Xu Bo , Liu Jing Tao , Ran Fu Xiang , Cui Jing Rong 
Published: March 2011

Abstractβ‐escin, a triterpene saponin, is one of the major active compounds extracted from horse chestnut (Aesculus hippocastanum) seed. Previous work has found that β‐escin sodium has antiinflammatory and antitumor effects. In the present study, we investigated its effect on cell proliferation and inducible nitric‐oxide synthase (iNOS) expression in human lung carcinoma A549 cells. β‐escin sodium (5–40 µg/mL) inhibited cytokine mixture (CM)‐induced nitric oxide (NO) production in A549 cells by reducing the expression of iNOS. β‐escin sodium suppressed phosphorylation and nuclear translocation of STAT1 (Tyr701) and STAT3 (Tyr705) induced by CM but did not affect the activation of c‐Jun and NF‐κB. β‐escin sodium inhibited the activation of protein tyrosine kinase JAK2. Pervanadate treatment reversed the β‐escin sodium‐induced downregulation of STAT3 and STAT1. β‐escin sodium treatment enhanced an activating phosphorylation of the phosphatase SHP2. Small interfering RNA‐mediated knockdown of SHP2 inhibited β‐escin sodium‐induced phospho‐STAT dephosphorylation. Moreover β‐escin sodium reduced the activation of p38 MAPK. Finally, β‐escin sodium inhibited the proliferation of A549 cells, did not change the cell membrane's permeability, nuclear morphology and size and the mitochondria's transmembrane potential of A549 cells. Taken together, these results demonstrate that β‐escin sodium could downregulate iNOS expression through inhibiting JAK/STAT signaling and p38 MAPK activation in A549 cells. β‐escin sodium has a marked antiproliferative effect on A549 cells at least in part by inhibiting the JAK/STAT signaling pathway, but not by a cytotoxic effect. β‐escin sodium would be useful as a chemopreventive agent or a therapeutic against inflammatory‐associated tumor. © 2011 Wiley Periodicals, Inc.
Resection and free tissue reconstruction of locally advanced oral cancer: Avoidance of lip split
Authors: Myers Larry L , Sumer Baran D , Truelson John M , Ahn Chul , Leach Joseph L 
Published: March 2011

AbstractThe purpose of this study is to report the outcomes of patients with locally advanced (T3–T4) oral cancers undergoing surgical resection and free tissue reconstruction without the lower lip‐split procedure. In this retrospective chart review, we analyzed 86 consecutive patients presenting between July 2000 and December 2009 at our university‐based, tertiary care medical center. The oral site distribution was: 73 (86%) oral cavity, 10 (12%) oropharynx, and 3 (2%) combined. The average specimen volume was 240.3 cm3 (range 17.5–3718 cm3). Sixty‐seven patients (78%) had widely clear histopathologic margins. Performing mandibulectomy had no advantage over maintaining mandible continuity to achieve clear margins (P = 0.97). Nineteen patients (22%) had focally involved microscopic margins; 10 (53%) soft tissue, seven (37%) bone, and two (10%) both. Thirty patients (35%) had postoperative complications, and 16 patients (19%) had a salivary fistula. The flaps used were: 39 fibula (45%), 25 radial forearm (29%), eight anterolateral thigh (9%), eight rectus abdominus (9%), three scapula (4%), and three iliac crest (4%). The average length of bone used was 9 cm (range 5–16 cm). The average soft tissue area was 99.7 cm2 (range 24–300 cm2). Nine patients (10%) had either partial or total flap loss. The lower lip‐split procedure for surgical exposure is unnecessary for both oncologic resection and reconstruction for locally advanced oral cancers. Clear margins, relatively facile flap inset with high success rates, and acceptable complication rates can be safely achieved in this patient population. © 2011 Wiley‐Liss, Inc. Microsurgery 2011.
Rat sciatic nerve repair with a poly‐lactic‐co‐glycolic acid scaffold and nerve growth factor releasing microspheres
Authors: de Boer Ralph , Knight Andrew M. , Borntraeger Andreas , Hébert‐Blouin Marie‐Noëlle , Spinner Robert J. , Malessy Martijn J.A. , Yaszemski Michael J , Windebank Anthony J. 
Published: March 2011

AbstractThe effect of microsphere delivered Nerve Growth Factor (NGF) in a poly‐lactic‐co‐glycolic‐acid (PLGA) 85/15 nerve conduit bridging a 10mm rat sciatic nerve gap was assessed, comparing nine groups (n = 6): PLGA conduits filled with saline, saline and NGF, saline with blank microspheres; four different NGF microspheres (5, 20, 50, and 100 mg/ml); an autologous graft and sciatic nerve gap. Histomorphometry, retrograde tracing, electrophysiology, and functional outcomes were evaluated up to 16 weeks. The autologous graft showed the largest fascicular area (0.65 mm2) and had a significantly greater number of myelinated fibers (P < 0.0001). Electrophysiology showed Compound Muscle Action Potential (CMAP) recordings for the autologous graft returning at 6 weeks after nerve transection, reaching their highest amplitude of 3.6 mV at endpoint. No significant differences were found in functional evaluation between groups or between conduits with microspheres and the saline filled conduit. A PLGA 85/15 nerve conduit is capable of sustaining nerve regeneration. The microsphere delivery system does not interfere with regeneration. © 2011 Wiley‐Liss, Inc. Microsurgery 2011.