bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial Transcriptomics-Aided Localization for Single-Cell Transcriptomics with STALocator
Authors: Li Shang , Shen Qunlun , Zhang Shihua 
Published: June 2024

AbstractSingle-cell RNA-sequencing (scRNA-seq) techniques can measure gene expression at the single-cell resolution but lack spatial information. The spatial transcriptomics (ST) techniques simultaneously provide gene expression data and spatial information. However, the data quality on the spatial resolution or gene coverage is still much lower than the single-cell transcriptomics data. To this end, we develop a Spatial Transcriptomics-Aided Locator for single-cell transcriptomics (STALocator) to localize single cells to corresponding ST data. Applications on simulated data showed that STALocator performed better than other localization methods from different angles. When applied to human brain scRNA-seq data and dorsolateral prefrontal cortex 10x Visium data, STALocator could robustly reconstruct the laminar organization of layer-associated cell types. Applications on scRNA-seq data and Spatial Transcriptomics data of human squamous cell carcinoma illustrated that STALocator could robustly reconstruct the relative spatial relationship between tumor-specific keratinocytes, microenvironment-associated cell populations, and immune cells. Moreover, STALocator could enhance gene expression patterns for Slide-seqV2 data and predict genome-wide gene expression data for FISH data, leading to the identification of more spatially variable genes and more biologically relevant GO terms compared to raw data.
Acetyl-CoA Synthetase Mutations S868G and V949I Do Not Confer Resistance to Antimalarial Drugsin vitroinPlasmodium falciparum
Authors: Zhao Wei , Xiang Zheng , Zeng Weilin , Qin Yucheng , Pan Maohua , Wu Yanrui , Duan Mengxi , Mou Ye , Liang Tao , Zhang Yanmei , Liu Cheng , Tang Xiuya , Huang Yaming , Yang Gongchao , Cui Liwang , Yang Zhaoqing 
Published: June 2024

ABSTRACTPlasmodium falciparumacetyl-CoA synthetase (PfACAS) protein is an important source of acetyl-CoA. We detected the mutations S868G and V949I in PfACAS by whole-genome sequencing analysis in some recrudescent parasites after antimalarial treatment with artesunate and dihydroartemisinin-piperaquine, suggesting that they may confer drug resistance. Using CRISPR/Cas9 technology, we engineered parasite lines carrying the PfACAS S868G and V949I mutations in two genetic backgrounds and evaluated their susceptibility to antimalarial drugs in vitro. The results demonstrated that PfACAS S868G and V949I mutations alone or in combination were not enough to provide resistance to antimalarial drugs.
FusOn-pLM: A Fusion Oncoprotein-Specific Language Model via Focused Probabilistic Masking
Authors: Vincoff Sophia , Goel Shrey , Kholina Kseniia , Pulugurta Rishab , Vure Pranay , Chatterjee Pranam 
Published: June 2024

AbstractFusion oncoproteins, a class of chimeric proteins arising from chromosomal translocations, drive and sustain various cancers, particularly those impacting children. Unfortunately, due to their intrinsically disordered nature, large size, and lack of well-defined, druggable pockets, they have been historically challenging to target therapeutically: neither small molecule-based methods nor structure-based approaches for binder design are strong options for this class of molecules. Recently, protein language models (pLMs) have demonstrated success at representing protein sequences with information-rich embeddings, enabling downstream design applications from sequence alone. However, no current pLM has been trained on fusion oncoprotein sequences and thus may not produce optimal representations for these proteins. In this work, we introduceFusOn-pLM, a novel pLM that fine-tunes the state-of-the-art ESM-2 model on fusion oncoprotein sequences. We specifically introduce a novel masked language modeling (MLM) strategy, employing a binding-site probability predictor to focus masking on key amino acid residues, thereby generating more optimal fusion oncoprotein-aware embeddings. Our model improves performance on both fusion oncoprotein-specific benchmarks and disorder prediction tasks in comparison to baseline ESM-2 representations, as well as manually-constructed biophysical embeddings, motivating downstream usage of FusOn-pLM embeddings for therapeutic design tasks targeting these fusions. We have made our model publicly available to the community athttps://huggingface.co/ChatterjeeLab/FusOn-pLM.
ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
Authors: Nagai Taylor Hanta , Mizoguchi Taiji , Wang Yanyan , Deik Amy , Bullock Kevin , Clish Clary B. , Xu Yu-Xin 
Published: June 2024

AbstractAngiopoietin-like 3 (ANGPTL3) is a key regulator of lipoprotein metabolism, known for its potent inhibition on intravascular lipoprotein and endothelial lipase activities. Recent studies have shed light on the cellular functions of ANGPTL3. However, the precise mechanism underlying its regulation of cellular lipid metabolism remains elusive. We recently reported that ANGPTL3 interacts with the chromatin regulator SMARCAL1, which plays a pivotal role in maintaining cellular lipid homeostasis. Here, through a combination of in vitro and in vivo functional analyses, we provide evidence that ANGPTL3 indeed influences cellular lipid metabolism. Increased expression of Angptl3 prompted the formation of lipid droplets (LDs) in response to slow growth conditions. Notably, under the conditions, Angptl3 accumulated within cytoplasmic peroxisomes, where it interacts with SmarcAL1, which translocated from nucleus as observed previously. This translocation induced changes in gene expression favoring triglyceride (TG) accumulation. Indeed,ANGPTL3gene knockout (KO) in human cells increased the expression of key lipid genes, which could be linked to elevated nuclear localization of SMARCAL1, whereas the expression of these genes decreased inSMARCAL1KO cells. Consistent with these findings, the injection of Angptl3 protein to mice led to hepatic fat accumulation derived from circulating blood, a phenotype likely indicative of its long-term effect on blood TG, linked to SmarcAL1 activities. Thus, our results suggest that the Angptl3-SmarcAL1 pathway may confer the capacity for TG storage in cells in response to varying growth states, which may have broad implications for this pathway in regulating energy storage and trafficking.
Elliptic anisotropy of open-charm hadrons from parton scatterings in
p
-Pb collisions at energies available at the CERN Large Hadron Collider
Authors: Tang Siyu , Lu Yuan , Zhang Chao , Wan RenZhuo 
Published: June 2024

The elliptic azimuthal anisotropy coefficient (v2) of open-charm hadrons at midrapidity (|η<1|) was studied in p-Pb collisions at sNN=8.16 TeV using a multiphase transport () model including an additional charm quark–antiquark pair production trigger. The model provides a simultaneous description of the measured pT spectrum and v2 of the D0 meson, as well as the v2 of light flavor meson Ks0. We found that the D0 and Ks0 v2 are both significantly affected by different parton scatterings among charm and light quarks. In addition, the predictions for the v2 of other charm hadrons including D+, Ds+, and Λc+ in p-Pb collisions are given for the first time. The v2 of open-charm hadrons reasonably follows the number-of-constituent-quark (NCQ) scaling up to 2.5 GeV, strongly indicating the importance of partonic degrees of freedom for the collectivity of heavy flavors in high-multiplicity p-Pb collisions. These findings may hint at the formation of deconfined matter in small collision systems, and provide referential value for future measurements of azimuthal anisotropy at energies available at the CERN Large Hadron Collider.

Published by the American Physical Society
2024
Cosmic string solution to the radio synchrotron background
Authors: Cyr Bryce , Chluba Jens , Acharya Sandeep Kumar 
Published: June 2024

We investigate the low-frequency spectral emission from a network of superconducting cosmic string loops in hopes of explaining the observed radio synchrotron background. After considering constraints from a variety of astrophysical and cosmological measurements, we identify a best-fit solution with string tension Gμ≃6.5×10−12 and current I≃2.5×106  GeV. This model yields a convincing fit to the data and may be testable in the near future by spectral distortion (TMS, BISOU) and 21 cm experiments (HERA, SKA, REACH). We also find that soft photon heating protects us against current constraints from global 21 cm experiments.

Published by the American Physical Society
2024
The relationship between happiness and consumption of brands and product categories in consumers' perception
Authors: Registro Gisele , Queiroz Mauricio Jucá de , Borini Felipe Mendes , dos Santos-Costa Lucas 
Published: June 2024

PurposeThe purpose of this article is to identify whether there is happiness in the consumption of brands and product categories and to clarify which provides more happiness: consuming the product itself or consuming the branded product.Design/methodology/approachThe research was a survey with 528 Brazilian consumers. Data were analyzed and interpreted through content analysis and regressions: linear, quantile and logistic.FindingsThe results show that there is happiness in the consumption of brands and product categories, with culture being the category that most provides happiness; also confirming that individuals who consume branded products are happier than those who consume the product itself.Research limitations/implicationsStudies confirm that there is happiness in consumption, but when we show that there is more happiness in the consumption of branded products than in not consuming the product itself, and when we identify which are the categories of products that bring the most happiness in an emerging country of Latin America, our article deepens and expands the previous literature.Practical implicationsWe suggest that companies associate their brands with culture to balance profit with sustainable purpose. For this, we provide a framework as a tool for this association.Originality/valueThe topic of our article is relevant, timely and current, its originality lies in confirming that those who consume the branded product are happier and those who consume the product itself are less happy, and also by identifying which categories provide the most happiness.
Performance barriers and competence development of graduate architects in construction contract administration (CCA): the mediating role of CCA skills
Authors: Si Yee Tiew 
Published: June 2024

PurposeEducation focused on the creation of a knowledge base but not on how to bring such knowledge into practice had caused graduate architects to encounter reality shock and fail to perform during building contract administration. Although the effect of performance barriers on competence development is empirically supported, less is known about the mechanisms that explain this effect. Overcoming barriers for graduate architects is crucial while supporting building contract administrators and acquiring professional qualifications. To address this issue, we propose the mediating role of CCA skills as a possible explanation of the relationship between performance barriers and competence development of graduate architects.Design/methodology/approachA survey was conducted among graduate architects in Klang Valley. In total, one hundred and twenty-seven usable questionnaires were returned and analyzed using descriptive analysis and regression analysis.FindingsMediation results indicate that performance barriers have a significant positive influence on competence development through CCA skills in claims and legal matters management, project management, communication and relationship management, quality assessment and management, and design management.Originality/valueThe present study contributes to the construction contract administration literature by integrating performance barriers and the CCA skills model. This advances our understanding of performance barriers and CCA skills engendering competence development of graduate architects.
Consumer segmentation in esports: clustering via consumption motivation
Authors: Rietz Julia , Hallmann Kirstin 
Published: June 2024

PurposeThe study aims to provide a reference for market segmentation in a relatively new market. Esports consumer profiles are developed based on consumption motives, structural factors, game genres, interests, demographics and behavioral intentions. It delivers managerial advice for a growing esports market.Design/methodology/approachA quantitative approach using an online survey was implemented to identify homogenous groups. The study employed the Motivation Scale for Sports Consumption (MSSC) to investigate the consumption motives of esports consumers. A two-step market segmentation was conducted based on the motives, applying hierarchical clustering. Moreover, descriptor variables were used to create distinct esports consumer profiles.FindingsThis research divides the esports market into four clusters based on MSSC, which is new and relevant in a constantly changing environment. The clusters are named Low Intention Novices, Leisure Warriors, Socializing Learners and Dedicated Enthusiasts.Originality/valueThis adds to the limited literature on esports market segmentation and highlights the theoretical and practical implications of the findings.
“We are openly, proudly Subjective … This history is important to our contemporary survival”: queer embodied knowledge and the curatorial work of ICT-based LGBTQIA+ history content creators
Authors: Wagner Travis L. 
Published: June 2024

PurposeThis article reports on findings from interviews with ICT-based content creators whose work focuses on documenting and curating queer history and culture. The research specifically examines how as amateur historians, the participant’s embodied knowledge plays a central role in how they engage with discourse about queer historical figures, methods of queer historiography and community accountability.Design/methodology/approachThe research deploys a queer constructivist framework to qualitatively gather and analyzes the semi-structured interviews of 31 North American content creators who curate digital project related to queer history and culture. The interviews were gathered between August 2022 and August 2023.FindingsThe research highlights how the subjectivity of queer embodiment aids, rather than hinders, participants' ability to collaborate with LGBTQIA+ communities while also addressing more significant ethical questions around intersectionality and inclusive historiographic work.Research limitations/implicationsThe content creators’ own positionality and commitments to community accountability and queer inclusivity fostered richer stories and historical documentation, while also helping make visible queer identity as affirming and valuable within queer culture. Additionally, practical implications include highlighting the value of ICT-based content within the distribution of educational and informational resources related to queer history.Originality/valueThis research offers an underexamined intersection of historiography and queer embodiment. While extensive scholarship on institutional and community-based historiography work exist the content creators interviewed within this study exist within the space of both, often using a combination of embodied knowledge and traditional curatorial work to translate between such spaces, inviting, in turn, new ways of thinking about queer archival knowledge.
Green bonds forecasting: evidence from pre-crisis, Covid-19 and Russian–Ukrainian crisis frameworks
Authors: Amri Amamou Souhir , Ben Daoud Mouna , Aguir Bargaoui Saoussen 
Published: June 2024

PurposeWithout precedent, green bonds confront, for the first time since their emergence, a twofold crisis context, namely the Covid-19-Russian–Ukrainian crisis period. In this context, this paper aims to investigate the connectedness between the two pioneering bond market classes that are conventional and treasury, with the green bonds market.Design/methodology/approachIn their forecasting target, authors use a Support Vector Regression model on daily S&P 500 Green, Conventional and Treasury Bond Indexes for a year from 2012 to 2022.FindingsAuthors argue that conventional bonds could better explain and predict green bonds than treasury bonds for the three studied sub-periods (pre-crisis period, Covid-19 crisis and Covid-19-Russian–Ukrainian crisis period). Furthermore, conventional and treasury bonds lose their forecasting power in crisis framework due to enhancements in market connectedness relationships. This effect makes spillovers in bond markets more sensitive to crisis and less predictable. Furthermore, this research paper indicates that even if the indicators of the COVID-19 crisis have stagnated and the markets have adapted to this rather harsh economic framework, the forecast errors persist higher than in the pre-crisis phase due to the Russian–Ukrainian crisis effect not yet addressed by the literature.Originality/valueThis study has several implications for the field of green bond forecasting. It not only illuminates the market participants to the best market forecasters, but it also contributes to the literature by proposing an unadvanced investigation of green bonds forecasting in Crisis periods that could help market participants and market policymakers to anticipate market evolutions and adapt their strategies to period specificities.
How does corporate hypocrisy undermine corporate reputation? The roles of corporate trust, affective commitment and CSR perception
Authors: Lee Luri , Hur Won-Moo 
Published: June 2024

Purpose
Corporate hypocrisy occurs when a discrepancy exists between corporate talk and actions. As companies assume more extensive economic, societal and global roles, they are more likely to encounter situations in which their words and actions are inconsistent. Therefore, a company’s ability to cope with such situations is becoming increasingly important. This study aims to examine the negative effects of corporate hypocrisy on corporate reputation. First, it reveals the underlying mechanisms by presenting corporate trust and affective commitment as parallel mediators. Additionally, it explores how corporate social responsibility (CSR) perceptions mitigate the indirect effects of corporate hypocrisy.

Design/methodology/approach
Data were collected through an online survey conducted at two time points in the context of Korean retail banking companies. A total of 313 respondents participated in a two-wave online survey using a multistage sampling technique to ensure a representative population sample. This study used the Mplus-based Hayes’ PROCESS Macro to test the research hypotheses.

Findings
The results show that corporate hypocrisy negatively affects corporate reputation by impeding customers’ corporate trust and affective commitment. These negative indirect effects are mitigated when customers’ perceptions of CSR are high.

Originality/value
By establishing a parallel moderated mediation model that captures the impact of corporate hypocrisy. To the best of the authors’ knowledge, this study presents important academic and managerial implications that have not been provided in the literature.
The influence of the reward culture on client-led service innovation in academic libraries of Uganda
Authors: Lwanga Elizabeth Namale Kawuma , Ngulube Patrick 
Published: June 2024

PurposeClient-led service innovation has gained currency in academic libraries owing to ever-shifting client preferences. Library clients are interested in determining which services libraries should offer to meet their information needs. Several studies have investigated service innovation or improvement in libraries with an emphasis on client involvement. However, it is not clear whether the reward culture influences client-focused service innovation in the context of Uganda.Design/methodology/approachThis multiple-case study triangulated data from a survey of 80 staff members, three semi-structured interviews conducted with three library heads, and data from institutional documents. Data were analysed quantitatively and qualitatively.FindingsThe results indicated that the financial reward culture did not influence client-led service innovation in any way; instead, non-financial rewards had an impact. Therefore, library managers need to appreciate that clients are no longer interested in financial rewards, but rather in non-financial rewards that have enduring value.Originality/valueThe study underscores the importance of client-led services in an information environment that has been invaded by industry 4.0 and calls upon librarians to use the opportunity to provide innovative services.
Effect of polymerization methods on surface roughness and hardness of commercially available self-curing acrylic resins: a comparative study
Authors: Rojas-Macedo Francisco Javier , Teutle-Coyotecatl Bernardo , Contreras-Bulnes Rosalía , Rodríguez-Vilchis Laura Emma , Reyes-Cervantes Eric , Velazquez-Enriquez Ulises 
Published: June 2024

Purpose
This paper aims to compare the surface roughness and hardness of three commercially available self-curing acrylic resins for dental use, under different polymerization conditions.

Design/methodology/approach
A comparative in vitro study was conducted using a convenience sample of 12 × 5 × 2 mm blocks with n = 40 for each material (Nic Tone, Arias Plus and Orthocryl®), with subgroups according to the polymerization method: conventional (C) and polymerization under ambient conditions (A). The surface roughness of the materials was measured using a profilometer; hardness was measured with a portable hardness tester. Additionally, surface morphology as well as particles size and morphology were evaluated with scanning electron microscopy.

Findings
There were significant differences in roughness and hardness values between the three self-curing acrylic resins (p < 0.05), as well as within each self-curing acrylic resin according to the polymerization method used (p < 0.05). The samples polymerized with the conventional method presented lower surface roughness and hardness values.

Originality/value
This study provides scientific evidence of values not provided by manufacturers in relation to the surface roughness and hardness of these materials, and all of them met the ideal minimum values of surface roughness, regardless of the polymerization technique used.