bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐targeted metabolite profiling of a fast neutron soybean mutant reveals an increase in sulfur‐containing metabolites
Authors: Islam Nazrul , Krishnan Hari B. , Slovin Janet , Natarajan Savithiry 
Published: May 2024

AbstractSoybean [Glycine max (L.) Merr] seed is a valuable source of raw materials of protein, oil, and metabolites for food, feed, and pharmaceutical industries. Using global metabolic profiling, we identified 718 non‐redundant metabolites of a fast neutron‐mediated soybean known to have 24 genes deleted and 15% more seed protein compared to wild‐type seed. Among the 554 identified metabolites in leaves and 629 identified in seed, 465 were found in both leaves and seed. Mapping of the deleted genes and identified metabolites indicated a complex perturbation of global metabolism involving glutamine‐arginine, aspartate, aromatic amino acid, glycolytic, and tricarboxylic acid cycle (TCA) pathways. The mutant seed showed a higher content of sulfur‐containing metabolites such as S‐methylmethionine, methionine, and cysteine compared to wild‐type seed. The gene deletions may alter carbon flow from glycolytic and TCA pathways, resulting in higher protein content and increased abundance of sulfur‐containing metabolites in the mutant seeds. The results provide valuable information for altering metabolic pathways to increase value‐added seed traits in soybeans.
The impact of China's new energy vehicle policies on corporate social responsibility: A textual big data analytics approach
Authors: Cao Chen , Lyu Yongming , Huang Jun , Luo Renjie , Huang Hancheng , Yu Guilan 
Published: May 2024

AbstractThis study combined the Latent Dirichlet Allocation topic model to explore the potential topics of new energy vehicle (NEV) policies and their impacts on the corporate social responsibility (CSR) of new energy vehicle enterprises. The results reveal that financial subsidies, infrastructure development, and environmental protection are the most popular topics. And these three popular topics have significant positive impacts on the CSR of new energy vehicle enterprises, with infrastructure construction having the most significant impact. Additionally, the intensity of the positive impact on large‐scale enterprises is more significant compared with small‐scale enterprises, and the impact on state‐owned enterprises is more remarkable than on private enterprises. Moreover, these three popular topics have more significant impacts on CSR in China's eastern region. Finally, we conclude this study by discussing the theoretical and practical implications of these findings.
Mechanism of Alkaline Earth Metal Amide Catalyzed Hydrogenation of Challenging Alkenes and Arenes
Authors: Qu Zheng‐Wang , Zhu Hui , Grimme Stefan 
Published: May 2024

AbstractRecently, bulky alkaline earth (Ae=Mg, Ca, Sr, Ba) metal amide complexes AeN“2 (N”=N[Si(iPr)3]2) are shown to be active for catalyzing the hydrogenation of unactivated alkenes and arenes, presumably via the monomer N“AeH as catalyst. In sharp contrast, our extensive DFT calculations disclose that the double Ae−H−Ae bridged dimer (N”AeH)2 is kinetically more favorable in catalytic hydrogenation with H2, although rate‐limited by the initial hydrogenolysis of AeN“2 to form the monomer N”AeH.
Role of ultrashort trapezoidal temporal pulse profile in laser wakefield acceleration in bubble regime
Authors: Kumar Sonu , Singh Dhananjay K. , Malik Hitendra K. 
Published: May 2024

AbstractA computational study is presented on laser wakefield acceleration (LWFA) in bubble regime with the use of ultrashort laser pulse propagating in an under‐dense plasma. The Particle‐In‐Cell simulations are performed to investigate the bubble wakefield acceleration of electrons realized by the incidence of an intense laser beam on cold, under‐dense plasma in two‐dimensional geometry. Different simulations are carried out and the results are compared for the beams with trapezoidal and Gaussian temporal pulse profiles having almost equal but slightly different energy contents. Focus is given to plasma density modulation, wakefield strength, electrons self‐injection, energy spectrum of accelerated electrons, the effect of an external longitudinal magnetic field and the study of pump depletion length and dephasing length in bubble regime with respect to these laser pulse profiles. Two limiting cases of the trapezoidal pulse, that is, triangular and rectangular pulses, are also discussed for better understanding of the role of steepness and plateau region in the laser pulse profile to the bubble wakefield acceleration. Since down ramp density gradient plays a crucial role for the generation of high‐quality electron beam in plasma wakefield acceleration as well as in LWFA, three different adjustments on the down ramp length determining three different density gradients are discussed for uncovering the role of trapezoidal laser pulse in LWFA.
Synthesis of new N‐(5,6‐methylenedioxybenzothiazole‐2‐yl)‐2‐[(substituted)thio/piperazine]acetamide/propanamide derivatives and evaluation of their AChE, BChE, and BACE‐1 inhibitory activities
Authors: Tutuş Beyzanur , Kaya Aybüke Züleyha , Baz Yonca , Evren Asaf Evrim , Sağlik Özkan Begüm Nurpelin , Yurttaş Leyla 
Published: May 2024

AbstractIn this study, the synthesis of N‐(5,6‐methylenedioxybenzothiazole‐2‐yl)‐2‐[(substituted)thio/piperazine]acetamide/propanamide derivatives (3a‐3k) and to investigate their acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and β‐secretase 1 (BACE‐1) inhibition activity were aimed. Mass, 1H NMR, and 13C NMR spectra were utilized to determine the structure of the synthesized compounds. Compounds 3b, 3c, 3f, and 3j showed AChE inhibitory activity which compound 3c (IC50 = 0.030 ± 0.001 µM) showed AChE inhibitory activity as high as the reference drug donepezil (IC50 = 0.0201 ± 0.0010 µM). Conversely, none of the compounds showed BChE activity. Compounds 3c and 3j showed the highest BACE‐1 inhibitory activity and IC50 value was found as 0.119 ± 0.004 µM for compound 3j whereas IC50 value was 0.110 ± 0.005 µM for donepezil, which is one of the reference substance. Molecular docking studies have been carried out using the data retrieved from the server of the Protein Data Bank (PDBID: 4EY7 and 2ZJM). Using in silico approach behavior active compounds (3c and 3j) and their binding modes clarified.
Coal dilemma: Is in situ clean power plant a way out?
Authors: Nie Baisheng , Jiang Liangliang , Liu Xianfeng , Deng Bozhi , Liu Peng , Zhang Hao , Song Suihong , Yan Ke , Zhang Jiansheng , Liu Ke , Chen Zhangxin , Farouq Ali S. M. 
Published: May 2024

AbstractThe global shift toward cleaner energy sources, driven by carbon neutrality goals and climate change urgency, faces a paradoxical rise in coal consumption despite efforts to expand alternative energies. Economic disparities drive reliance on coal, particularly in underdeveloped regions where affordability and accessibility dictate energy choices. To address this, innovative approaches such as harnessing controlled pulverized coal detonation for in situ clean power plants are proposed. These plants offer compact, efficient, and sustainable energy generation with reduced emissions and minimal environmental impact. In addition, a comparison of costs, efficiency, and environmental impact with conventional methods showcases the potential of this novel technology to transform coal‐based energy into a green and viable solution.
Activitybudget and foraging patterns ofNubian giraffe (Giraffa camelopardalis camelopardalis) in Lake Nakuru National Park, Kenya
Authors: Gitau Consolata G. , Mbau Judith S. , Ngugi Robinson K. , ngumbi Emmanuel , Muneza Arthur B. 
Published: May 2024

AbstractThe activity budget of giraffe in various African populations has been studied extensively, revealing that it is affected by body size, foraging patterns, and sex. Foraging patterns show an animal's feeding choices in its environment and are influenced by resource availability, competition, and predation risk. The ability of giraffe to survive and reproduce is significantly impacted by the variation in activity budget and foraging across different ecosystems. Our study focused on evaluating the seasonal activity budgets and foraging patterns of Nubian giraffe in Lake Nakuru National Park, Kenya. We used the scan sampling method to record the activity budget of giraffe which included foraging, movement, resting, and drinking water. We then evaluated if activities varied with the seasons. A total of 11,280 activities were documented, with 4560 (40.4%) occurring during the dry season and 6720 (59.6%) during the wet season. Foraging accounted for 53% of the time budget during the dry season, but increased to 57% during the wet season. There was a slight drop in records of movement (22%; n = 995 of 4560) and resting (25%; n = 1145 of 4560) from the dry season to the wet season (20%; n = 1375 out of 6720 and 22%; n = 1515 of 6720). During the dry season, females (53%) foraged longer than males (47%), whereas males (44%) had longer resting periods than females (56%). Giraffe frequently fed on Vachellia xanthophloea (67%; n = 4136 of 6215 foraging records), Maytenus senegalensis (19%), and Solanum incanum (9%) over both seasons. Overall, seasons had little impact on giraffe activity time budgets and foraging patterns in Lake Nakuru National Park. A better insight into the behavioural patterns of this subspecies will allow managers to enhance the protection and conservation of the species and its habitat. Heavy foraging on Vachellia by giraffe at LNNP has been associated with a population decline in number, so perhaps planting more of this species in LNNP could promote a rebound in numbers.
Cryptic diversity and speciation in an endemic copepod crustacean Harpacticella inopinata within Lake Baikal
Authors: Kochanova Elena , Mayor Tatyana , Väinölä Risto 
Published: May 2024

AbstractAncient lakes are hotspots of species diversity, posing challenges and opportunities for exploration of the dynamics of endemic diversification. Lake Baikal in Siberia, the oldest lake in the world, hosts a particularly rich crustacean fauna, including the largest known species flock of harpacticoid copepods with some 70 species. Here, we focused on exploring the diversity and evolution within a single nominal species, Harpacticella inopinata Sars, 1908, using molecular markers (mitochondrial COI, nuclear ITS1 and 28S rRNA) and a set of qualitative and quantitative morphological traits. Five major mitochondrial lineages were recognized, with model‐corrected COI distances of 0.20–0.37. A concordant pattern was seen in the nuclear data set, and qualitative morphological traits also distinguish a part of the lineages. All this suggests the presence of several hitherto unrecognized cryptic taxa within the baikalian H. inopinata, with long independent histories. The abundances, distributions and inferred demographic histories were different among taxa. Two taxa, H. inopinata CE and H. inopinata CW, were widespread on the eastern and western coasts, respectively, and were largely allopatric. Patterns in mitochondrial variation, that is, shallow star‐like haplotype networks, suggest these taxa have spread through the lake relatively recently. Three other taxa, H. inopinata RE, RW and RW2, instead were rare and had more localized distributions on either coast, but showed deeper intraspecies genealogies, suggesting older regional presence. The rare taxa were often found in sympatry with the others and occasionally introgressed by mtDNA from the common ones. The mitochondrial divergence between and within the H. inopinata lineages is still unexpectedly deep, suggesting an unusually high molecular rate. The recognition of true systematic diversity in the evaluation and management of ecosystems is important in hotspots, as it is everywhere else, while the translation of the diversity into a formal taxonomy remains a challenge.
Denitrative Formal [5+1] Cycloaddition of 1‐(2‐Nitrophenyl)‐2‐Yn‐1‐Ones with Thioacetamide under Transition‐Metal‐Free Conditions: Synthesis of Thiochromen‐4‐Ones
Authors: Xu Yao , Wang Wenjun , Fan Hui , Liao Xiaoming , Yang Zhuoran , Yu Lei , Hong Chan Philip Wai , Zhang Xiaoxiang 
Published: May 2024

AbstractA new strategy for thiochromen‐4‐one synthesis that relies on the denitrative formal [5+1] cycloaddition of 1‐(2‐nitrophenyl)‐2‐yn‐1‐ones with thioacetamide is described. Achieved under reaction conditions that did not require transition metal catalysis, nor the exclusion of air or moisture, the reaction is suggested to involve exploiting the propensity of thioacetamide to undergo hydrolysis under basic conditions. This is follows by addition of the ensuing sulfide anion to the 1,3‐alkynone and a nucleophilic aromatic substitution and denitrative rearomatization cascade to give the S‐heterocyclic product in yields of 41–99 %.
Prospective study of epilepsy with generalized tonic–clonic seizures alone: Clinical features, response to treatment, and likelihood of medication withdrawal
Authors: Jaafar Fatima , Wazne Jaafar , Hmaimess Ghassan , Nasreddine Wassim , Beydoun Ayman , Shatila AbdelRahman , Beydoun Ahmad 
Published: May 2024

AbstractObjectiveThis prospective study aimed to delineate the demographics, natural progression, and treatment response of patients newly diagnosed with epilepsy with generalized tonic–clonic seizures alone (EGTCA). Furthermore, our objective includes assessing the seizure recurrence rate post antiseizure medication (ASM) discontinuation within this cohort, alongside exploring predictive factors for seizure relapse.MethodsThe study cohort, derived from an ongoing, prospective, multicenter investigation on children and adults with new‐onset unprovoked seizures, included consecutive patients enrolled between March 2010 and March 2020, and meeting mandatory ILAE criteria for EGTCA diagnosis. Participants underwent a 3‐h sleep‐deprived video‐EEG recording along with an epilepsy protocol brain magnetic resonance imaging (MRI) with repeat EEG at each follow‐up. Cumulative time‐dependent probabilities of seizure recurrence were calculated using Kaplan–Meier survival analysis. Logistic regression identified variables associated with seizure recurrence following ASM taper.ResultsEighty‐nine patients with a median age of 16 years were included, constituting 31% of those diagnosed with an idiopathic generalized epilepsy. Regarding the circadian distribution of seizures, 59.6% of patients exclusively experienced diurnal seizures, 12.4% exclusively nocturnal, and 28.1% experienced both diurnal and nocturnal seizures. Generalized spike–wave discharges (GSWD) were present in the initial EEG of 88% of patients. A GTC recurred in 14% of patients treated with ASM compared with 73% of untreated patients (p < 0.00001). ASM discontinuation was attempted in 50 patients after a median treatment duration of 3 years, with 44% experiencing a recurrence. Patient‐initiated taper and a mixed circadian seizure pattern independently predicted a higher likelihood of recurrence post‐ASM discontinuation.SignificanceOur findings underscore the importance of prompt treatment upon the diagnosis of EGTCA. Notably, lifelong treatment may not be imperative; patients seizure‐free for at least 2 years, with the absence of GSWD on EEG, often maintained seizure freedom after ASM withdrawal, especially with physician‐initiated tapering.Plain Language SummarySeizures in individuals diagnosed with “epilepsy with generalized tonic‐clonic seizures alone” (EGTCA) typically start during adolescence and often respond well to antiseizure medications. An electroencephalogram, which measure brain waves, will show abnormal discharges in most patients with EGTCA. Lifelong treatment with antiseizure medication is not necessary for everyone with EGTCA; approximately, 40% can successfully stop treatment without facing seizure recurrence. Patients who stop medication on their own have a higher risk of seizures returning compared with those who undergo cessation under a doctor's supervision.
Research on coal and gangue segmentation based on MFCCM‐Mask R‐CNN
Authors: Cao Zhenguan , Li Zhuoqin , Fang Liao , Li Jinbiao , Yang Haixia , Hui Donggao 
Published: May 2024

AbstractIntelligent sorting of coal and gangue is of great significance to the intelligent construction of coal mines as well as green development. In this study, we propose a coal and gangue segmentation method with an improved classical segmentation network Mask R‐CNN, denoted as Multichannel Forward‐Linked Confusion Convolution Module (MFCCM)‐Mask R‐CNN. First, we design a MFCCM to construct the feature extraction network by stacking, second, we design a multiscale high‐resolution feature pyramid network structure to realize multipath fusion of feature information to enhance the position and contour information of the target, and finally, we propose a multiscale Mask head to enhance the diversity of information, and capture the more representative and unique features. Training and testing models using self‐built RGB coal and gangue data sets, the accuracy of the improved algorithm reaches 97.38%, which is an improvement of 1.66% compared to the original model. Compared with other segmentation models Unet, Deeplab V3+, Yoloact, Yolov7, and the model after replacing the backbone network, the MFCCM‐Mask R‐CNN has higher precision and recall, and can more accurately realize the efficient segmentation of coal and gangue.
Teasing out the Effects of Natural Stressors at Chemically Contaminated Sites
Authors: Nichols Elizabeth R. , Burton G. Allen , Lavoie Dan , Tortomasi Jon , Cervi Eduardo , Hudson Michelle , Brown Steve 
Published: May 2024

Abstract
Aquatic ecosystems are often impacted by a multitude of stressors, many of which are introduced by a combination of anthropogenic activities such as agricultural development, urbanization, damming, and industrial discharge. Determining the primary stressors responsible for ecological impairments at a site can be complex and challenging; however, it is crucial for making informed management decisions. Improper diagnosis of an impaired system can lead to misguided attempts at remediation, which can be both time consuming and costly. We focused on the development, implementation, and evaluation of methodologies that, in combination, allowed us to identify the primary stressors. These included a four-phase, weight-of-evidence (WOE) assessment including in situ Toxicity Identification and Evaluation (iTIE) testing, physicochemical and macrobenthos characterization, reciprocal sediment transplants, and laboratory and in situ toxicity testing. The contaminants of concern (COCs) at the site were elevated levels of ammonia, chloride, pH, and total dissolved solids in groundwater upwellings into a high-quality waterway. Reciprocal transplants of site sediments and nearby reference sediments and traditional benthic sampling showed impaired benthic indices and multiple stations around a contaminated industrial settling basin. Impaired stations had elevated COCs in groundwaters but exhibited a steep vertical concentration gradient, with concentrations decreasing near the sediment–surface water interface. We describe Phase 4 of the study, which focused on teasing out the role of dissolved oxygen sags in benthic macroinvertebrate responses. Extensive submerged and emergent macrophytes, algae, and cyanobacteria co-occurred at the impaired sites and increased throughout the summer. Laboratory testing suggested that ammonia and pH were possibly toxic at the sites, based on groundwater concentrations. The in situ toxicity testing, however, showed toxicity occurring even at stations with low levels of COCs concurrently with large diurnal fluxes in dissolved oxygen (DO). A final phase using a type of iTIE approach utilized limnocorrals with and without aeration and with in situ toxicity measures using Hyalella azteca. The Phase 4 assessment revealed that low DO levels were primarily responsible for impaired benthic communities, and COC upwellings were diluted at the sediment–water interface to nontoxic levels. These findings will allow for improved management decisions for more efficient and effective restoration activities. Environ Toxicol Chem 2024;43:1524–1536. © 2024 SETAC
Following Regulation, Imidacloprid Persists and Flupyradifurone Increases in Nontarget Wildlife
Authors: English Simon G. , Bishop Christine A. , Bieber Matthias , Elliott John E. 
Published: May 2024

Abstract
After regulation of pesticides, determination of their persistence in the environment is an important indicator of effectiveness of these measures. We quantified concentrations of two types of systemic insecticides, neonicotinoids (imidacloprid, acetamiprid, clothianidin, thiacloprid, and thiamethoxam) and butenolides (flupyradifurone), in off-crop nontarget media of hummingbird cloacal fluid, honey bee (Apis mellifera) nectar and honey, and wildflowers before and after regulation of imidacloprid on highbush blueberries in Canada in April 2021. We found that mean total pesticide load increased in hummingbird cloacal fluid, nectar, and flower samples following imidacloprid regulation. On average, we did not find evidence of a decrease in imidacloprid concentrations after regulation. However, there were some decreases, some increases, and other cases with no changes in imidacloprid levels depending on the specific media, time point of sampling, and site type. At the same time, we found an overall increase in flupyradifurone, acetamiprid, thiamethoxam, and thiacloprid but no change in clothianidin concentrations. In particular, flupyradifurone concentrations observed in biota sampled near agricultural areas increased twofold in honey bee nectar, sevenfold in hummingbird cloacal fluid, and eightfold in flowers after the 2021 imidacloprid regulation. The highest residue detected was flupyradifurone at 665 ng/mL (parts per billion [ppb]) in honey bee nectar. Mean total pesticide loads were highest in honey samples (84 ± 10 ppb), followed by nectar (56 ± 7 ppb), then hummingbird cloacal fluid (1.8 ± 0.5 ppb), and least, flowers (0.51 ± 0.06 ppb). Our results highlight that limited regulation of imidacloprid does not immediately reduce residue concentrations, while other systemic insecticides, possibly replacement compounds, concurrently increase in wildlife. Environ Toxicol Chem 2024;43:1497–1508. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Guidelines for separation of natural extracellular vesicles
Authors: Liang Duo , Li Yu , Li Jiaxuan , Yang Shen , Su Wentao 
Published: May 2024

AbstractExtracellular vesicles (EVs) are found everywhere in different biofluids such as blood, saliva, urine, cerebrospinal fluid, and breast milk. They have sizes ranging from 20 to 400 nm and contain a wide range of cargo, including membrane proteins, periplasmic proteins, and nucleic acids. EVs possess the remarkable ability to diffuse, traverse the mucus layer, migrate directly to other tissues, and interact with various cells of the host immune system. Consequently, researchers have harnessed their potential as delivery carriers for novel immuno‐drugs or bioactive components. The separation of EVs from food can be considered a safe and effective method for harnessing their beneficial effects. In this guide, we present isolation protocols for natural EVs derived from plants, dairy products, cells, and microbes. This includes steps for raw material processing or culture as well as conditions for separation and purification. Moreover, these protocols can serve as a foundation for preparing and isolating engineered EVs. In summary, the utilization of natural EVs presents a vast array of opportunities in the fields of biotechnology, biomedicine, and food nutrition.