bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can a Regional Climate Model Improve the Ability to Forecast the North American Monsoon?
Authors: Castro Christopher L. , Chang Hsin-I , Dominguez Francina , Carrillo Carlos , Schemm Jae-Kyung , Henry Juang Hann-Ming 
Published: June 2012

Abstract
Global climate models are challenged to represent the North American monsoon, in terms of its climatology and interannual variability. To investigate whether a regional atmospheric model can improve warm season forecasts in North America, a retrospective Climate Forecast System (CFS) model reforecast (1982–2000) and the corresponding NCEP–NCAR reanalysis are dynamically downscaled with the Weather Research and Forecasting model (WRF), with similar parameterization options as used for high-resolution numerical weather prediction and a new spectral nudging capability. The regional model improves the climatological representation of monsoon precipitation because of its more realistic representation of the diurnal cycle of convection. However, it is challenged to capture organized, propagating convection at a distance from terrain, regardless of the boundary forcing data used. Dynamical downscaling of CFS generally yields modest improvement in surface temperature and precipitation anomaly correlations in those regions where it is already positive in the global model. For the North American monsoon region, WRF adds value to the seasonally forecast temperature only in early summer and does not add value to the seasonally forecast precipitation. CFS has a greater ability to represent the large-scale atmospheric circulation in early summer because of the influence of Pacific SST forcing. The temperature and precipitation anomaly correlations in both the global and regional model are thus relatively higher in early summer than late summer. As the dominant modes of early warm season precipitation are better represented in the regional model, given reasonable large-scale atmospheric forcing, dynamical downscaling will add value to warm season seasonal forecasts. CFS performance appears to be inconsistent in this regard.
Diagnosing Seasonal Vegetation Impacts on Evapotranspiration and Its Partitioning at the Catchment Scale during SMEX04–NAME
Authors: Vivoni Enrique R. 
Published: June 2012

Abstract
A fundamental problem in ecohydrology is diagnosing impacts of vegetation dynamics on the catchment response. This study uses a distributed hydrologic model and remote sensing data to evaluate the effects of seasonal vegetation greening on the basin water balance and the partitioning of evapotranspiration ET into soil evaporation, transpiration, and evaporation of intercepted water. Using remotely sensed data, updates are made to model vegetation parameters related to radiation, interception, and transpiration as ecosystems respond to precipitation during the North American monsoon (NAM). Comparisons of simulations with static and seasonally varying vegetation parameters reveal lower ET but higher vegetation-mediated ET losses because of the greening. Sensitivity analyses indicate that vegetation fraction is the primary control on ET and its partitioning, while interception parameters play a secondary role. As a result, spatial patterns in ET partitioning in the catchment exhibit a strong signature of vegetation fraction, though fine (coarse)-scale influences of soil moisture (radiation) are also observed. Vegetation-mediated ET losses were significant in large fractions of the catchment and exhibited ecosystem-dependent seasonal evolutions. The numerical simulations presented here provide the first spatially explicit estimates of ET partitioning accounting for vegetation dynamics obtained from remotely sensed data at the catchment scale.
Efficiency of four sampling methods used to detect two common nosocomial pathogens on textiles
Authors: Rabuza Urška , Šostar-Turk Sonja , Fijan Sabina 
Published: June 2012

Detecting microorganisms on textiles is useful for many purposes, for example to determine the bioburden before laundering, assess the reduction in bacterial counts in connection with various laundry processes, or trace transfer routes in infection control investigations. Therefore a validated, reproducible and rational method is needed. For sampling microorganisms on textile surfaces the most commonly used method is the contact plate method using RODAC plates, first described by Hall and Hartnett followed by the swab sampling technique. Both methods can only capture microorganisms on the surface of the textiles while microorganisms that have penetrated into the deeper structure of the material will not be detected. In our research the contact plate method and the swabbing technique were compared with two wash-off methods. For the first wash-off method the destructive elution method was used, where microorganisms were eluted from the fabrics by shaking the fabrics for a certain time in an elution medium. For the fourth sampling method a nondestructive method that included a compact test device called Morapex® was used, which is based on forced desorption by pressing the microorganisms through the fabric without destroying the fabric. In our research, two types of microorganisms were included ( Klebsiella pneumoniae and Staphylococcus aureus) that cause common nosocomial infections. The aim of this study was to compare the efficiency of the four sampling methods for detecting microorganisms on textiles and to determine the lowest concentration, which can still be detected. The percentage of microorganisms that were detected by both elution methods was substantially higher than by sampling of fabrics with the contact plate method or swabbing. It can be concluded that a nondestructive method using a modified Morapex® device can be applied for quick determination of the hygienic condition of textiles.
Dermatophytosis and papular eosinophilic/mastocytic dermatitis (urticaria pigmentosa-like dermatitis) in three Devon Rex cats
Authors: Colombo Silvia , Scarampella Fabia , Ordeix Laura , Roccabianca Paola 
Published: June 2012

Presenting signs: Three Devon Rex cats were presented with multiple erythematous papules, occasionally associated with crusting and hyperpigmentation, with a linear distribution on the head, neck, chest and abdomen. One cat also had multifocal alopecia with hyperpigmentation on the dorsum. Diagnosis and treatment: Clinical and histopathological features were suggestive of papular eosinophilic/mastocytic dermatitis (urticaria pigmentosa-like dermatitis). In all cases, dermatophytosis was diagnosed: in cases 1 and 2 there was histopathological evidence of dermatophytosis, while fungal culture was positive for Microsporum canis in cases 2 and 3. In all cats, lesions disappeared following antifungal treatment. Clinical significance: Papular eosinophilic/mastocytic dermatitis in Devon Rex cats may represent either an atypical presentation of dermatophytosis or a clinical and histological reaction pattern to various diseases, including dermatophytosis and allergic diseases. Clinical differentiation is crucial as there are important implications regarding treatment and, in particular, the use of glucocorticoids, which are contraindicated in cases of dermatophytosis.
Arterial Thromboembolism
Authors: Fuentes Virginia Luis 
Published: June 2012

Practical relevance: Feline arterial thromboembolism (ATE) is a common but devastating complication of myocardial disease, often necessitating euthanasia. A combination of endothelial dysfunction and blood stasis in the left atrium leads to local platelet activation and thrombus formation. Embolisation of the thrombus results in severe ischaemia of the affected vascular bed. With the classic ‘saddle thrombus’ presentation of thrombus in the terminal aorta, the diagnosis can usually be made by physical examination. The prognosis is poor for cats with multiple limbs affected by severe ischaemia, but much better where only one limb is affected or motor function is present. Patient group: Cats with left atrial enlargement secondary to cardiomyopathy are typically predisposed, although cats with hyperthyroidism, pulmonary neoplasia and supravalvular mitral stenosis may also be at risk. Management: Analgesia is the main priority, and severe pain should be managed with methadone or a fentanyl constant rate infusion. Congestive heart failure (CHF) requires treatment with furosemide, but tachypnoea due to pain can mimic signs of CHF. Thrombolytic therapy is not recommended, but antithrombotic treatment should be started as soon as possible. Aspirin and clopidogrel are well tolerated. Evidence base: Several observational studies of ATE have been reported. No randomised, blinded, controlled studies have been reported in cats at risk, for either treatment or prevention of ATE, although such a study comparing aspirin and clopidogrel in cats is currently under way.
Feline Eosinophilic Granuloma Complex(ITIES)
Authors: Buckley Laura , Nuttall Tim 
Published: June 2012

Practical relevance: The feline eosinophilic granuloma complex (EGC) comprises a group of clinically well recognised but poorly understood dermatoses that are common in cats. In many cases, lesions are severe and can be accompanied by varying degrees of (and sometimes considerable) pruritus and/or pain. In addition, lesions can be chronic and recurrent. It is, therefore, important to achieve a prompt and accurate diagnosis in order to provide optimal, often life-long, treatment for affected cats. Patient group: There is no age predisposition or well documented breed predilection for the development of EGC lesions in cats. Some studies have reported a possible female predisposition, but this has not been consistently documented. Clinical challenges: The clinical diagnosis of EGC lesions is usually straightforward, but investigation of the potential underlying aetiology can pose a challenge for the clinician. Information on the indication for various diagnostic tests and their interpretation is lacking, and the tendency for these cases to be managed with chronic medical intervention prior to achieving a definitive diagnosis can further complicate the interpretation of any diagnostic investigation. In addition, successful therapeutic management of these cases can be challenging. Some cats suffer only a single episode of disease that resolves with treatment, while others have recurrent lesions and some of these can be refractory to treatment. The individual variation in both the clinical nature of the disease and the response to therapy could be related to disease severity, but could also be explained by differences in the underlying aetiopathogenesis. Evidence base: This article reviews the published literature to discuss the complex aetiology of the EGC and present an overview of the different clinical presentations and diagnosis. A further and particular aim has been to provide some evidence-based recommendations for the management of this unusual group of dermatoses.
Advanced Imaging
Authors: Lamb Christopher R , David Frédéric H 
Published: June 2012

Summary: The primary purpose of diagnostic imaging is to enable clinicians to make correct decisions about a patient’s diagnosis and severity of its condition and, thereby, to contribute to effective and efficient treatment. Advanced imaging modalities are inherently better detectors of morphological lesions than radiography; hence, they offer the possibility of more accurate diagnosis and staging, with correspondingly better patient outcomes. However, there is a lack of evidence that better outcomes are being realised for veterinary patients. Furthermore, increased use of advanced imaging carries the possibility of increased misuse, primarily through unnecessary studies that contribute little to patient management other than increased costs. Scope: This article is intended to be relevant to all veterinary species. Advanced imaging of cats is not sufficiently developed as a specific discipline that a balanced review could be based purely on feline examples or references.
Effects of Cold Modality Application With Static and Intermittent Pneumatic Compression on Tissue Temperature and Systemic Cardiovascular Responses
Authors: Holwerda Seth W. , Trowbridge Cynthia A. , Womochel Kathryn S. , Keller David M. 
Published: June 2012

Background: In the therapeutic setting, cryotherapy with varying levels of intermittent cyclical compression often replaces an ice bag and elastic wrap. However, little is known about the cardiovascular strain and tissue temperature decreases associated with cooling and intermittent compression. Hypothesis: The authors hypothesized that higher levels of intermittent compression will result in greater reductions of tissue temperature and that all cold modalities will cause acute increases in cardiovascular strain. Design: Experimental crossover repeated measure design. Methods: Ten healthy subjects (23 ± 3 years) volunteered for 4 cryotherapy sessions (30-minute treatments with 30-minute passive recovery). Treatments included ice with elastic wrap and Game Ready (GR) with no, medium (5-50 mmHg), and high compression (5-75 mmHg). Throughout the experiment, oral, skin surface, and intramuscular quadriceps temperatures were measured along with mean arterial pressure, heart rate, rate pressure product, forearm blood flow, and forearm vascular conductance. Results: Mean arterial pressure increased up to 5 minutes ( P < 0.05). Forearm blood flow and forearm vascular conductance decreased after baseline ( P < 0.05), but there were no differences between treatments. Peak intramuscular changes from baseline were −14 ± 2°C (ice), −11 ± 6°C (GRHIGH), −10 ± 5°C (GRMED), and −7 ± 3°C (GRNO). Ice cooled the muscle the most, while GR with medium and high compression cooled more than GR without compression ( P < 0.05). Conclusions: The application of cold and intermittent pneumatic compression using GR did not produce acute cardiovascular strain that exceeded the strain produced by standard ice bags/elastic wrap treatment. Greater temperature decreases are achieved with medium- and high-pressure settings when using the GR system. Clinical Relevance: Type of cold and amount of compression affect tissue cooling in healthy lean subjects. All tested cold modalities caused acute increases in cardiovascular strain; however, these increases are no more than what healthy subjects experience with the onset of exercise.
L-leucine improves the anemia and developmental defects associated with Diamond-Blackfan anemia and del(5q) MDS by activating the mTOR pathway
Authors: Payne Elspeth M. , Virgilio Maria , Narla Anupama , Sun Hong , Levine Michelle , Paw Barry H. , Berliner Nancy , Look A. Thomas , Ebert Benjamin L. , Khanna-Gupta Arati 
Published: June 2012

Abstract
Haploinsufficiency of ribosomal proteins (RPs) has been proposed to be the common basis for the anemia observed in Diamond-Blackfan anemia (DBA) and myelodysplastic syndrome with loss of chromosome 5q [del(5q) MDS]. We have modeled DBA and del(5q) MDS in zebrafish using antisense morpholinos to rps19 and rps14, respectively, and have demonstrated that, as in humans, haploinsufficient levels of these proteins lead to a profound anemia. To address the hypothesis that RP loss results in impaired mRNA translation, we treated Rps19 and Rps14-deficient embryos with the amino acid L-leucine, a known activator of mRNA translation. This resulted in a striking improvement of the anemia associated with RP loss. We confirmed our findings in primary human CD34+ cells, after shRNA knockdown of RPS19 and RPS14. Furthermore, we showed that loss of Rps19 or Rps14 activates the mTOR pathway, and this is accentuated by L-leucine in both Rps19 and Rps14 morphants. This effect could be abrogated by rapamycin suggesting that mTOR signaling may be responsible for the improvement in anemia associated with L-leucine. Our studies support the rationale for ongoing clinical trials of L-leucine as a therapeutic agent for DBA, and potentially for patients with del(5q) MDS.
Combined gene expression and DNA occupancy profiling identifies potential therapeutic targets of t(8;21) AML
Authors: Lo Miao-Chia , Peterson Luke F. , Yan Ming , Cong Xiuli , Jin Fulai , Shia Wei-Jong , Matsuura Shinobu , Ahn Eun-Young , Komeno Yukiko , Ly Minh , Ommen Hans B. , Chen I-Ming , Hokland Peter , Willman Cheryl L. , Ren Bing , Zhang Dong-Er 
Published: June 2012

AbstractChromosome translocation 8q22;21q22 [t(8;21)] is commonly associated with acute myeloid leukemia (AML), and the resulting AML1-ETO fusion proteins are involved in the pathogenesis of AML. To identify novel molecular and therapeutic targets, we performed combined gene expression microarray and promoter occupancy (ChIP-chip) profiling using Lin−/Sca1−/cKit+ cells, the major leukemia cell population, from an AML mouse model induced by AML1-ETO9a (AE9a). Approximately 30% of the identified common targets of microarray and ChIP-chip assays overlap with the human t(8;21)–gene expression molecular signature. CD45, a protein tyrosine phosphatase and a negative regulator of cytokine/growth factor receptor and JAK/STAT signaling, is among those targets. Its expression is substantially down-regulated in leukemia cells. Consequently, JAK/STAT signaling is enhanced. Re-expression of CD45 suppresses JAK/STAT activation, delays leukemia development, and promotes apoptosis of t(8;21)–positive cells. This study demonstrates the benefit of combining gene expression and promoter occupancy profiling assays to identify molecular and potential therapeutic targets in human cancers and describes a previously unappreciated signaling pathway involving t(8;21) fusion proteins, CD45, and JAK/STAT, which could be a potential novel target for treating t(8;21) AML.
A phase 2 trial of standard-dose cyclophosphamide, doxorubicin, vincristine, prednisone (CHOP) and rituximab plus bevacizumab for patients with newly diagnosed diffuse large B-cell non-Hodgkin lymphoma: SWOG 0515
Authors: Stopeck Alison T. , Unger Joseph M. , Rimsza Lisa M. , LeBlanc Michael , Farnsworth Brent , Iannone Maria , Glenn Martha J. , Fisher Richard I. , Miller Thomas P. 
Published: June 2012

AbstractS0515 was a phase 2 trial to determine whether the addition of bevacizumab to cyclophosphamide, doxorubicin, vincristine, prednisone (CHOP) plus rituximab (R-CHOP) would improve progression-free survival (PFS) without adding significant toxicity in patients with newly diagnosed advanced diffuse large B-cell lymphoma. A total of 73 patients were enrolled. For the 64 eligible patients, median age was 68 years, and 60% had International Prognostic Index scores more than or equal to 3. The observed 1- and 2-year PFS estimates were 77% and 69%, respectively. These PFS estimates were not statistically different from the expected PFS for this population if treated with R-CHOP alone. Grade 3 or higher toxicities were observed in 81% of patients, including 2 grade 5 events. The majority of serious toxicities were hematologic but also included 5 patients with gastrointestinal perforations, 4 patients with thrombotic events, and 11 patients who developed grade 2 or 3 left ventricular dysfunction. Higher baseline urine VEGF and plasma VCAM levels correlated with worse PFS and overall survival. In conclusion, the addition of bevacizumab to R-CHOP chemotherapy was not promising in terms of PFS and resulted in increased serious toxicities, especially cardiac and gastrointestinal perforations. This study is registered at www.clinicaltrials.gov as #NCT00121199.
A new biologic prognostic model based on immunohistochemistry predicts survival in patients with diffuse large B-cell lymphoma
Authors: Perry Anamarija M. , Cardesa-Salzmann Teresa M. , Meyer Paul N. , Colomo Luis , Smith Lynette M. , Fu Kai , Greiner Timothy C. , Delabie Jan , Gascoyne Randy D. , Rimsza Lisa , Jaffe Elaine S. , Ott German , Rosenwald Andreas , Braziel Rita M. , Tubbs Raymond , Cook James R. , Staudt Louis M. , Connors Joseph M. , Sehn Laurie H. , Vose Julie M. , López-Guillermo Armando , Campo Elias , Chan Wing C. , Weisenburger Dennis D. 
Published: June 2012

Abstract
Biologic factors that predict the survival of patients with a diffuse large B-cell lymphoma, such as cell of origin and stromal signatures, have been discovered by gene expression profiling. We attempted to simulate these gene expression profiling findings and create a new biologic prognostic model based on immunohistochemistry. We studied 199 patients (125 in the training set, 74 in the validation set) with de novo diffuse large B-cell lymphoma treated with rituximab and CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) or CHOP-like therapies, and immunohistochemical stains were performed on paraffin-embedded tissue microarrays. In the model, 1 point was awarded for each adverse prognostic factor: nongerminal center B cell–like subtype, SPARC (secreted protein, acidic, and rich in cysteine) < 5%, and microvascular density quartile 4. The model using these 3 biologic markers was highly predictive of overall survival and event-free survival in multivariate analysis after adjusting for the International Prognostic Index in both the training and validation sets. This new model delineates 2 groups of patients, 1 with a low biologic score (0-1) and good survival and the other with a high score (2-3) and poor survival. This new biologic prognostic model could be used with the International Prognostic Index to stratify patients for novel or risk-adapted therapies.
Genome-wide SNP scan of pooled DNA reveals nonsense mutation in FGF20 in the scaleless line of featherless chickens
Authors: Wells Kirsty L , Hadad Yair , Ben-Avraham Danny , Hillel Jossi , Cahaner Avigdor , Headon Denis J 
Published: June 2012

Abstract

Background
Scaleless (sc/sc) chickens carry a single recessive mutation that causes a lack of almost all body feathers, as well as foot scales and spurs, due to a failure of skin patterning during embryogenesis. This spontaneous mutant line, first described in the 1950s, has been used extensively to explore the tissue interactions involved in ectodermal appendage formation in embryonic skin. Moreover, the trait is potentially useful in tropical agriculture due to the ability of featherless chickens to tolerate heat, which is at present a major constraint to efficient poultry meat production in hot climates. In the interests of enhancing our understanding of feather placode development, and to provide the poultry industry with a strategy to breed heat-tolerant meat-type chickens (broilers), we mapped and identified the sc mutation.

Results
Through a cost-effective and labour-efficient SNP array mapping approach using DNA from sc/sc and sc/+ blood sample pools, we map the sc trait to chromosome 4 and show that a nonsense mutation in FGF20 is completely associated with the sc/sc phenotype. This mutation, common to all sc/sc individuals and absent from wild type, is predicted to lead to loss of a highly conserved region of the FGF20 protein important for FGF signalling. In situ hybridisation and quantitative RT-PCR studies reveal that FGF20 is epidermally expressed during the early stages of feather placode patterning. In addition, we describe a dCAPS genotyping assay based on the mutation, developed to facilitate discrimination between wild type and sc alleles.

Conclusions
This work represents the first loss of function genetic evidence supporting a role for FGF ligand signalling in feather development, and suggests FGF20 as a novel central player in the development of vertebrate skin appendages, including hair follicles and exocrine glands. In addition, this is to our knowledge the first report describing the use of the chicken SNP array to map genes based on genotyping of DNA samples from pooled whole blood. The identification of the sc mutation has important implications for the future breeding of this potentially useful trait for the poultry industry, and our genotyping assay can facilitate its rapid introgression into production lines.