bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decision support—Do you have it?
Authors: Hatch Toby , Stratton William , Lawson Raef A. , Desroches Denis 
Published: July 2009

AbstractMany organizations want to be able to make better decisions using their cost and profitability system, yet are thwarted from doing so for a variety of reasons. This article shows how organizations can improve their current systems. It discusses some unachieved goals, and also demonstrates how you can successfully support business decisions using a cost and profitability system. © 2009 Wiley Periodicals, Inc.
Protein cytoskeleton and overexpression of Na+,K+‐ATPase in opossum kidney cells
Authors: Silva Elisabete , Soares‐da‐Silva Patrício 
Published: July 2009

AbstractPrevious studies have shown that over time in culture opossum kidney (OK) cells are endowed with increased Na+,K+‐ATPase activity and expression (Silva et al., , J Membr Biol 212:163–175; Silva and Soares‐da‐Silva, , Am J Physiol Regul Integr Comp Physiol 293:R1764–R1770). The present work evaluated the cytoskeleton reorganization in OK cells at passages 40 and 80 in culture and its possible relationship with membrane transport proteins and cell morphology. It is shown that OK cells with 80 passages in culture have increased size, internal complexity, and total protein expression. In OK cells with 80 passages in culture the use of in‐cell western showed that ezrin/radixin/moesin complex was increased by 20%. The most abundant ankyrin‐G isoform in OK cells with 40 passages was the ∼200/220 kDa isoform, whereas in OK cells with 80 passages the most abundant isoform was the ∼170 kDa isoform. The spectrin‐βII ∼240 kDa isoform, the predominant isoform in OK cells with 40 passages, was marginally detected in OK cells with 80 passages. Besides Na+,K+‐ATPase, GLUT2, and NHE3 expression was also significantly increased in OK cells with 80 passages. It is concluded that the prolonged cell passaging of OK cells results in an interesting and valuable experimental model to analyze the reorganization of the renal cell cytoskeleton proteins and its relationship with transporter and signaling membrane proteins. J. Cell. Physiol. 221: 318–324, 2009. © 2009 Wiley‐Liss, Inc.
New methods for chicken embryo manipulations
Authors: El‐Ghali Nissrine , Rabadi Maes , Ezin Akouavi M. , De Bellard Maria Elena 
Published: July 2009

AbstractThe capacity to image a growing embryo while simultaneously studying the developmental function of specific molecules provides invaluable information on embryogenesis. However, until recently, this approach was accomplished with difficulty both because of the advanced technology needed and because an easy method of minimizing damage to the embryo was unavailable. Here, we present a novel way of adapting the well‐known EC culture of whole chick embryos to time‐lapse imaging and to functional molecular studies using blocking agents. The novelty of our method stems from the ability to apply blocking agents ex ovo as well as in ovo. We were able to study the function of a set of molecules by culturing developing embryos ex ovo in tissue culture media containing these molecules or by injecting them underneath the live embryo in ovo. The in ovo preparation is particularly valuable, because it extends the period of time during which the developmental function of the molecule can be studied and it provides an easy, reproducible method for screening a batch of molecules. These new techniques will prove very helpful in visualizing and understanding the role of specific molecules during embryonic morphogenesis, including blood vessel formation. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.
Whole body regeneration in a colonial ascidian, Botrylloides violaceus
Authors: Brown Federico D. , Keeling Elena L. , Le Anna D. , Swalla Billie J. 
Published: July 2009

AbstractColonial ascidians are the only chordates to undergo whole body regeneration (WBR), the ability to form an entirely new individual from the peripheral vasculature. Here we describe WBR in Botrylloides violaceus, a colonial ascidian that reliably regenerates after ablation of all zooids and buds of young colonies. During early regeneration several buds develop within the tunic vasculature, but only one continues development into a complete zooid. We describe some of the first events of vascular budding leading to the vesicle stage with phase contrast microscopy, time‐lapse video recording and detailed histological studies of regenerating colonies. The first conspicuous stage of vascular budding is when a single‐layered sphere of cells becomes enclosed by vascular epithelium. We report the appearance of Piwi‐positive cells in hemocytes surrounding the regenerates. We observed an increase of proliferating cell nuclear antigen (PCNA)‐positive cells in circulatory hemocytes in late regenerates, and found double‐labeled nuclear expression with Piwi in a subset of large circulatory cells. We rarely found Piwi or PCNA in differentiating tissues during vascular budding, suggesting that cells that form the epithelial tissues during budding and WBR originate mostly from circulatory hemocyte precursors. We propose that multiple stem cell types are circulating within B. violaceus and that they undergo proliferation in the peripheral vasculature before differentiating into epithelial tissues for all three germ layers during WBR. J. Exp. Zool. (Mol. Dev. Evol.) 312B:885–900, 2009. © 2009 Wiley‐Liss, Inc.
Synthesis and characteristic of 5,6‐dinitro and 5,6‐diaminobenzimidazolone‐2
Authors: Yang Zehui , Wang Jiarong , Li Linglan , Ye Chuping , Liu Hui 
Published: July 2009

Abstract
magnified image5,6‐Dinitro and 5,6‐diaminobenzimidazolone‐2 can be synthesized at a sufficiently high purity and yield to permit its large scale production in an economically feasible manner. The results of our studies derived optimum conditions for the nitration process necessary to obtain pure 5,6‐dinitriobenzimidazolone‐2. J. Heterocyclic Chem. (2009).
Formation of an unusual product in the reaction of a 1,2,5‐thiadiazolidine 1,1‐dioxide‐derived thioether with sulfuryl chloride
Authors: Dou Dengfeng , Talaty Erach R. , Moore Curtis E. , Bullinger John C. , Eichhorn David M. , Groutas William C. 
Published: July 2009

Abstract
magnified imageThe treatment of a 1,2,5‐thiadiazolidine 1,1‐dioxide‐derived phenylthiomethyl ether with sulfuryl chloride yielded an unexpected dimeric product whose structure was determined using X‐ray crystallography. A plausible mechanism for the formation of this product is proposed. J. Heterocyclic Chem. (2009).
Substituent‐dependence of photophysical properties of trans‐2‐styrylpyridazin‐3(2H)‐ones
Authors: Kim Bo Ram , Cho Su‐Dong , Lee Hyung‐Geun , Yim Heung‐Seop , Kim Min‐Jung , Hwang Jaeyoung , Park Song‐Eun , Kim Jeum‐Jong , Jung Kwang‐Ju , Yoon Yong‐Jin 
Published: July 2009

Abstract
magnified imageThe photophysical behavior of trans‐2‐styrylpyridazin‐3(2H)‐ones 3 strongly depend on the number and the position of substituents in the phenyl ring in THF, methylene chloride, acetonitrile and methanol. The absorption spectra of 3 containing the electron‐donating substituents at the para‐position show the red‐shift, whereas the spectra of 3 containing the electron‐withdrawing substituents show the blue‐shift. For the trans‐2‐(p‐substituted‐styryl)pyridazin‐3(2H)‐ones 3b, 3c, 3d, 3e, 3f, 3g, 3h and 3k, 3l, 3m, 3n, 3o, the magnitude of the solvatochromic shifts and the shape of the fluorescence spectra depend on the number and/or the position of substituents in benzene ring. The emission maximum of trans‐2‐styrylpyridazin‐3(2H)‐ones involving the electron‐donating group is larger than one of trans‐2‐styrylpyridazin‐3(2H)‐ones involving the electron‐withdrawing group in the phenyl ring. The magnitude of the emission maximum is roughly parallel to the relative electron‐withdrawing ability of the substituents of the phenyl ring. J. Heterocyclic Chem., (2009).
Evaluation of local MCP‐1 and IL‐12 nanocoatings for infection prevention in open fractures
Authors: Li Bingyun , Jiang Bingbing , Dietz Matthew J. , Smith E. Suzanne , Clovis Nina B. , Rao K. Murali Krishna 
Published: July 2009

AbstractThe increasing incidence of bacterial infection and the appearance of Staphylococcus aureus (S. aureus) strains that are resistant to commonly used antibiotics has made it important to develop non‐antibiotic approaches for infection prevention. The aim of this study was to develop local monocyte chemoattractant protein‐1 (MCP‐1) and interleukin‐12 p70 (IL‐12 p70) therapies to prevent S. aureus infection by enhancing the recruitment and activation of macrophages, which are believed to play an important role in infection prevention as the first line of defense against invading pathogens. Nanocoating systems for MCP‐1 and IL‐12 p70 deliveries were prepared, and their release characteristics desirable for infection prevention in open fractures were explored. Local MCP‐1 therapy reduced S. aureus infection and influenced white blood cell populations, and local IL‐12 p70 treatment had a more profound effect on preventing S. aureus infection. No synergistic relationship in decreasing S. aureus infection was observed when MCP‐1 and IL‐12 p70 treatments were combined. This reported new approach may reduce antibiotic use and antibiotic resistance. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:48–54, 2010
Raman spectroscopy as a potential method for the detection of extremely halophilic archaea embedded in halite in terrestrial and possibly extraterrestrial samples
Authors: Fendrihan Sergiu , Musso Maurizio , Stan‐Lotter Helga 
Published: July 2009

AbstractEvidence for the widespread occurrence of extraterrestrial halite, particularly on Mars, has led to speculations on the possibility of halophilic microbial forms of life; these ideas have been strengthened by reports of viable haloarchaea from sediments of geological age (millions of years). Raman spectroscopy, being a sensitive detection method for future astrobiological investigations onsite, has been used in the current study for the detection of nine different extremely halophilic archaeal strains which had been embedded in laboratory‐made halite crystals in order to simulate evaporitic conditions. The cells accumulated preferentially in tiny fluid inclusions, in simulation of the precipitation of salt in natural brines. FT‐Raman spectroscopy using laser excitation at 1064 nm and dispersive micro Raman spectroscopy at 514.5 nm were applied. The spectra showed prominent peaks at 1507, 1152 and 1002 cm−1 which are attributed to haloarchaeal C50 carotenoid compounds (mainly bacterioruberins). Their intensity varied from strain to strain at 1064‐nm laser excitation. Other distinguishable features were peaks due to peptide bonds (amide I, amide III) and to nucleic acids. No evidence for fatty acids was detected, consistent with their general absence in all archaea.These results contribute to a growing database on Raman spectra of terrestrial microorganisms from hypersaline environments and highlight the influence of the different macromolecular composition of diverse strains on these spectra. Copyright © 2009 John Wiley & Sons, Ltd.
Effects of latitude and weather conditions on sugars, fruit acids and ascorbic acid in currant (Ribes sp.) cultivars
Authors: Zheng Jie , Kallio Heikki , Yang Baoru 
Published: July 2009

AbstractBACKGROUND: Sugars, acids and vitamin C are essential components influencing the sensory and nutritional properties of currants (Ribes sp.). In this study the effects of growth latitude and weather conditions on these components in red, white and green currant cultivars were systematically investigated for the first time.RESULTS: Red Dutch (a red Ribes rubrum cultivar) contained more malic acid but fewer sugars and had a lower sugar/acid ratio than Vertti (a green Ribes nigrum cultivar) and White Dutch (a white R. rubrum cultivar). Fructose (27–56 g L−1 juice) and glucose (21–54 g L−1) were the major sugars in all currant samples, but sucrose (7–20 g L−1) was present only in Vertti. Vertti contained the most ascorbic acid. Sugars, malic acid and the sugar/acid ratio in Red Dutch were 11–28% higher in southern Finland than in northern Finland. The corresponding values in Vertti were 6–16% lower in the south than in the north, but no differences were found in White Dutch. As the relative humidity decreased, the amounts of citric acid and total acid increased in all cultivars, while the amount of sugars increased in Vertti but decreased in White Dutch and Red Dutch. In Red Dutch, high temperature and radiation levels increased the sugar content.CONCLUSION: The influence of latitude and weather conditions on qualitative parameters of currants varies with the cultivar. This study provides important guidance for currant cultivation and for further physiological and enzymological studies. Copyright © 2009 Society of Chemical Industry
Laparoscopic versus open gastrectomy for gastric cancer patients with COPD
Authors: Chang Hao‐Ming , Lee Sang‐Woong , Nomura Eiji , Tanigawa Nobuhiko 
Published: July 2009

AbstractBackgroundPatients with chronic obstructive pulmonary disease (COPD) have a higher risk of developing postoperative pulmonary complications. Laparoscopic gastrectomy (LG) is accepted as a standard treatment for early gastric cancer. We sought to compare the clinical presentation and complications associated with LG and open gastrectomy (OG) to define the role of LG in the treatment of patients with gastric cancer and COPD.MethodsThis was a retrospective cohort study of patients with gastric cancer and COPD who underwent gastrectomy from January 2005 to December 2007. Patients were divided into LG group and OG group according to the treatment guidelines. The stage of COPD was determined by the FEV1 ratio.ResultsThere were 104 patients enrolled in this study. No significant differences were observed in preoperative pulmonary function between the two groups, and most patients (99/104, 95%) were in COPD stages I and II. The changes in end‐tidal CO2 (P = 0.012) and PaCO2 (P < 0.001) during surgery were statistically significant in the LG group. There were no significant differences in incidence of postoperative pulmonary complications between the LG (5/61, 8%) and OG (4/43, 9%) groups.ConclusionsLG can be performed safely for patients with gastric cancer and COPD in stage I and II. J. Surg. Oncol. 2009;100:456–458. © 2009 Wiley‐Liss, Inc.
Modification of approach for totally implantable venous access device decreases rate of complications
Authors: Karanlik Hasan , Kurul Sidika 
Published: July 2009

AbstractBackgroundThe aim of this study was to analyze the potential utilization of external jugular vein (EJV) cutdown approach for totally implantable venous access device (TIVAD) placement.MethodsThis is a prospective, non‐randomized, observational, uni‐institutional study. All patients (n = 451) were first subjected to attempted subclavian vein (SV) percutaneous approach. If SV percutaneous approach was unsuccessful after multiple puncture in group A and maximum two puncture in group B, an ipsilateral EJV cutdown approach was attempted.ResultsFour hundred four patients (89.6%) underwent TIVAD placement via SV percutaneous approach. Of those 47 patients in which SV percutaneous approach was not successful, 8 patients in group A and 39 patients in group B, TIVAD was placed via ipsilateral EJV cutdown approach. The avarage of operative time was 17.9 ± 8.5 min in group A and 18 ± 7.3 min in group B (P > 0.05). The early complications of group A were significantly higher than that of group B (P = 0.02).ConclusionsTIVAD placement by the SV percutaneous approach is safe and fast, and its success rate is very high. By avoiding the immediate complications associated with the percutaneous approach, the EJV cutdown has to be considered a valid, safe, and suitable alternative when the SV percutaneous approach unsuccessful after two needle puncture. J. Surg. Oncol. 2009;100:279–283. © 2009 Wiley‐Liss, Inc.
Participant reactions to a pretreatment research assessment during a treatment outcome study for PTSD
Authors: Resick Patricia A. , Iverson Katherine M. , Artz Caroline E. 
Published: July 2009

AbstractParticipants' reactions to pretreatment assessments have not been studied as part of a randomized clinical trial (RCT) for posttraumatic stress disorder (PTSD). The current study examined participants' reactions in women with PTSD who completed pretreatment assessments during an RCT. We assessed participant reactions (N = 100) to a pretreatment assessment that included self‐report questionnaires, interviews, and psychophysiological assessment. Results indicated that participation in pretreatment assessment was well tolerated as measured by participants' reports of distress, interest level, perceptions of the appropriateness of assessment length, and willingness to participate in a similar assessment in the future. Participating in lengthy pretreatment assessments did not adversely impact treatment participation or outcome.
Miscibility of Polynorbornene/Poly(styrene‐co‐hydroxystyrene) Binary Blend Based on Hydrogen‐Bonding Interaction
Authors: Tanaka Shimon , Nishida Haruo , Endo Takeshi 
Published: July 2009

AbstractThe miscibility and hydrogen bonding interaction of polynorbornene (PNB)/poly(styrene‐co‐hydroxystyrene) (PST‐HST) binary blends were investigated by differential scanning calorimetry and Fourier transform infrared (FT‐IR) spectroscopy. PNB was found to be miscible with PST‐HSTs containing HST unit compositions of 5, 10, 20, and 28 mol‐%, showing a single glass transition, whereas it was immiscible with polystyrene, exhibiting two glass transitions. The hydrogen bonding interaction between the hydroxyl group of PST‐HST and the carbonyl group of PNB was revealed by FT‐IR spectroscopy and plays a key role in the miscibility of PNB/PST‐HST blends.magnified image
Glucosamine Hydrochloride Functionalized Water‐Soluble Conjugated Polyfluorene: Synthesis, Characterization, and Interactions with DNA
Authors: Chen Qi , Cheng Qian‐Yi , Zhao Yan‐Chao , Han Bao‐Hang 
Published: July 2009

AbstractNovel glucosamine hydrochloride functionalized water‐soluble conjugated polyfluorene was easily synthesized through Cu(I)‐catalyzed azide/alkyne “click” ligation and Suzuki coupling polymerization. The water‐solubility and biocompatibility of the polymer were improved after grafting glucosamine hydrochloride to the side chains of the conjugated polymer. As a fluorescent model system of chitosan, its interaction with single‐stranded DNA was studied by spectrofluorometric titration.magnified image
Insulin‐like growth factor type I receptor gene expression and obesity in esophageal adenocarcinoma
Authors: Zhao Ronghua , Macdonald Kimberley , Casson Alan G. 
Published: July 2009

AbstractThe objective of this exploratory study was to evaluate the role of the insulin‐like growth factor I receptor (IGF‐IR) in esophageal adenocarcinoma (EADC). Using quantitative PCR, we studied IGF‐IR mRNA expression in 52 well‐characterized surgically resected EADC and matched histologically normal esophageal tissues, and examined IGF‐IR expression levels in relation to clinicopathologic characteristics, body mass index (BMI), and the common IGF‐IR polymorphism (G1013A), recently proposed to modify risk of obesity for EADC. Expression levels of IGF‐IR mRNA were not significantly different between EADC and matched histologically normal esophageal epithelia. Although no significant associations were found between IGF‐IR expression and BMI, tumor differentiation, stage or survival, when stratified by genotype, patients with the polymorphic A variant had significantly higher IGF‐IR expression in EADC tissues compared with matched normal epithelia. These findings suggest that G1013A most likely modulates IGF‐IR function, possibly by influencing gene transcription or mRNA stability, and represents a plausible mechanistic link underlying the association between obesity and esophageal malignancy. © 2009 Wiley‐Liss, Inc.
Numerical integration on the sphere and its effect on the material symmetry of constitutive equations—A comparative study
Authors: Ehret A. E. , Itskov M. , Schmid H. 
Published: July 2009

AbstractIn the present paper, we consider a class of constitutive models based on numerical integration on the unit sphere. The directional behaviour of the quadrature schemes and its effect on the symmetry properties of these constitutive models are studied by subjecting the set of integration points on the sphere to arbitrary rigid rotations. We investigate a number of recently proposed integration schemes in application to a full network model of rubber elasticity and to an exponential model for soft tissues. In order to assess and compare these schemes, statistical methods are presented and applied. The analysis discloses a number of integration schemes that offer a good compromise between the numerical error and the number of integration points. However, as a general result it turns out that numerical integration is prone to introduce strong anisotropy into originally isotropic constitutive equations, in particular, for highly non‐linear integrand functions. The consequences for application of the investigated class of constitutive models in finite element calculations are highlighted in a benchmark‐like numerical example. Copyright © 2009 John Wiley & Sons, Ltd.
Activated macrophages in the tumour microenvironment—dancing to the tune of TLR and NF‐κB
Authors: Hallam Simon , Escorcio‐Correia Monica , Soper Robin , Schultheiss Anne , Hagemann Thorsten 
Published: July 2009

AbstractA large number of variables have been identified which appear to influence macrophage phenotype within the tumour microenvironment. These include reciprocal chemical and physical interactions with tumour cells and with non‐malignant cells of the tumour microenvironment, tissue oxygen tension, and the origin and prior experience of the particular macrophage population. In this review we outline the key evidence for these influences and consider how macrophage phenotype is acquired and the relevance of the TLR–NF‐κB pathway. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.