bluuebunny/crossref_metadata_2025_split huggingface dataset:
Navigating in the Fog
Authors: Barton Frederick D. , Hayden Shannon , von Hippel Karin 
Published: May 2012

AbstractReligion features prominently in many of the major foreign policy challenges facing the new Obama administration, notably in Afghanistan, Iran, Iraq, Nigeria, Pakistan, Somalia, and of course, the Middle East as a whole. Yet beyond the experts, most government officials and implementing partners still do not have the requisite tools nor necessary understanding of the issues to factor religion into policy and practice in an appropriate manner. This chapter discusses U.S. engagement with religion in two parts. The first outlines the difficulties and challenges in conflict zones, and discusses the legal issues that arise. The second proposes concrete recommendations, which are intended to help clarify guidance and harmonize practice. Because conflict zones are complex and fluid environments, and religion can be interpreted very differently in each situation, it is not possible to develop a generic framework that can be applied in all contexts. Aid workers, soldiers, diplomats, and other practitioners need new tools, but they must also be prepared to work with ambiguity and to rely on their own judgment.
A Constitutive Expressed Phosphate Transporter, OsPht1;1, Modulates Phosphate Uptake and Translocation in Phosphate-Replete Rice
Authors: Sun Shubin , Gu Mian , Cao Yue , Huang Xinpeng , Zhang Xiao , Ai Penghui , Zhao Jianning , Fan Xiaorong , Xu Guohua 
Published: May 2012

AbstractA number of phosphate (Pi) starvation- or mycorrhiza-regulated Pi transporters belonging to the Pht1 family have been functionally characterized in several plant species, whereas functions of the Pi transporters that are not regulated by changes in Pi supply are lacking. In this study, we show that rice (Oryza sativa) Pht1;1 (OsPT1), one of the 13 Pht1 Pi transporters in rice, was expressed abundantly and constitutively in various cell types of both roots and shoots. OsPT1 was able to complement the proton-coupled Pi transporter activities in a yeast mutant defective in Pi uptake. Transgenic plants of OsPT1 overexpression lines and RNA interference knockdown lines contained significantly higher and lower phosphorus concentrations, respectively, compared with the wild-type control in Pi-sufficient shoots. These responses of the transgenic plants to Pi supply were further confirmed by the changes in depolarization of root cell membrane potential, root hair occurrence, 33P uptake rate and transportation, as well as phosphorus accumulation in young leaves at Pi-sufficient levels. Furthermore, OsPT1 expression was strongly enhanced by the mutation of Phosphate Overaccumulator2 (OsPHO2) but not by Phosphate Starvation Response2, indicating that OsPT1 is involved in the OsPHO2-regulated Pi pathway. These results indicate that OsPT1 is a key member of the Pht1 family involved in Pi uptake and translocation in rice under Pi-replete conditions.
Environmental Stresses of Field Growth Allow Cinnamyl Alcohol Dehydrogenase-Deficient Nicotiana attenuata Plants to Compensate for their Structural Deficiencies
Authors: Kaur Harleen , Shaker Kamel , Heinzel Nicolas , Ralph John , Gális Ivan , Baldwin Ian T. 
Published: May 2012

Abstract
The organized lignocellulosic assemblies of cell walls provide the structural integrity required for the large statures of terrestrial plants. Silencing two CINNAMYL ALCOHOL DEHYDROGENASE (CAD) genes in Nicotiana attenuata produced plants (ir-CAD) with thin, red-pigmented stems, low CAD and sinapyl alcohol dehydrogenase activity, low lignin contents, and rubbery, structurally unstable stems when grown in the glasshouse (GH). However, when planted into their native desert habitat, ir-CAD plants produced robust stems that survived wind storms as well as the wild-type plants. Despite efficient silencing of NaCAD transcripts and enzymatic activity, field-grown ir-CAD plants had delayed and restricted spread of red stem pigmentation, a color change reflecting blocked lignification by CAD silencing, and attained wild-type-comparable total lignin contents. The rubbery GH phenotype was largely restored when field-grown ir-CAD plants were protected from wind, herbivore attack, and ultraviolet B exposure and grown in restricted rooting volumes; conversely, it was lost when ir-CAD plants were experimentally exposed to wind, ultraviolet B, and grown in large pots in growth chambers. Transcript and liquid chromatography-electrospray ionization-time-of-flight analysis revealed that these environmental stresses enhanced the accumulation of various phenylpropanoids in stems of field-grown plants; gas chromatography-mass spectrometry and nuclear magnetic resonance analysis revealed that the lignin of field-grown ir-CAD plants had GH-grown comparable levels of sinapaldehyde and syringaldehyde cross-linked into their lignins. Additionally, field-grown ir-CAD plants had short, thick stems with normal xylem element traits, which collectively enabled field-grown ir-CAD plants to compensate for the structural deficiencies associated with CAD silencing. Environmental stresses play an essential role in regulating lignin biosynthesis in lignin-deficient plants.
Endoplasmic Reticulum-Quality Control Chaperones Facilitate the Biogenesis of Cf Receptor-Like Proteins Involved in Pathogen Resistance of Tomato
Authors: Liebrand Thomas W.H. , Smit Patrick , Abd-El-Haliem Ahmed , de Jonge Ronnie , Cordewener Jan H.G. , America Antoine H.P. , Sklenar Jan , Jones Alexandra M.E. , Robatzek Silke , Thomma Bart P.H.J. , Tameling Wladimir I.L. , Joosten Matthieu H.A.J. 
Published: May 2012

Abstract
Cf proteins are receptor-like proteins (RLPs) that mediate resistance of tomato (Solanum lycopersicum) to the foliar pathogen Cladosporium fulvum. These transmembrane immune receptors, which carry extracellular leucine-rich repeats that are subjected to posttranslational glycosylation, perceive effectors of the pathogen and trigger a defense response that results in plant resistance. To identify proteins required for the functionality of these RLPs, we performed immunopurification of a functional Cf-4-enhanced green fluorescent protein fusion protein transiently expressed in Nicotiana benthamiana, followed by mass spectrometry. The endoplasmic reticulum (ER) heat shock protein70 binding proteins (BiPs) and lectin-type calreticulins (CRTs), which are chaperones involved in ER-quality control, were copurifying with Cf-4-enhanced green fluorescent protein. The tomato and N. benthamiana genomes encode four BiP homologs and silencing experiments revealed that these BiPs are important for overall plant viability. For the three tomato CRTs, virus-induced gene silencing targeting the plant-specific CRT3a gene resulted in a significantly compromised Cf-4-mediated defense response and loss of full resistance to C. fulvum. We show that upon knockdown of CRT3a the Cf-4 protein accumulated, but the pool of Cf-4 protein carrying complex-type N-linked glycans was largely reduced. Together, our study on proteins required for Cf function reveals an important role for the CRT ER chaperone CRT3a in the biogenesis and functionality of this type of RLP involved in plant defense.
Examination of the Distribution of Arsenic in Hydrated and Fresh Cowpea Roots Using Two- and Three-Dimensional Techniques
Authors: Kopittke Peter M. , de Jonge Martin D. , Menzies Neal W. , Wang Peng , Donner Erica , McKenna Brigid A. , Paterson David , Howard Daryl L. , Lombi Enzo 
Published: May 2012

Abstract
Arsenic (As) is considered to be the environmental contaminant of greatest concern due to its potential accumulation in the food chain and in humans. Using novel synchrotron-based x-ray fluorescence techniques (including sequential computed tomography), short-term solution culture studies were used to examine the spatial distribution of As in hydrated and fresh roots of cowpea (Vigna unguiculata ‘Red Caloona’) seedlings exposed to 4 or 20 µm arsenate [As(V)] or 4 or 20 µm arsenite. For plants exposed to As(V), the highest concentrations were observed internally at the root apex (meristem), with As also accumulating in the root border cells and at the endodermis. When exposed to arsenite, the endodermis was again a site of accumulation, although no As was observed in border cells. For As(V), subsequent transfer of seedlings to an As-free solution resulted in a decrease in tissue As concentrations, but growth did not improve. These data suggest that, under our experimental conditions, the accumulation of As causes permanent damage to the meristem. In addition, we suggest that root border cells possibly contribute to the plant’s ability to tolerate excess As(V) by accumulating high levels of As and limiting its movement into the root.
Arabidopsis ECERIFERUM9 Involvement in Cuticle Formation and Maintenance of Plant Water Status
Authors: Lü Shiyou , Zhao Huayan , Des Marais David L. , Parsons Eugene P. , Wen Xiaoxue , Xu Xiaojing , Bangarusamy Dhinoth K. , Wang Guangchao , Rowland Owen , Juenger Thomas , Bressan Ray A. , Jenks Matthew A. 
Published: May 2012

Abstract
Mutation of the ECERIFERUM9 (CER9) gene in Arabidopsis (Arabidopsis thaliana) causes elevated amounts of 18-carbon-length cutin monomers and a dramatic shift in the cuticular wax profile (especially on leaves) toward the very-long-chain free fatty acids tetracosanoic acid (C24) and hexacosanoic acid (C26). Relative to the wild type, cer9 mutants exhibit elevated cuticle membrane thickness over epidermal cells and cuticular ledges with increased occlusion of the stomatal pore. The cuticular phenotypes of cer9 are associated with delayed onset of wilting in plants experiencing water deficit, lower transpiration rates, and improved water use efficiency measured as carbon isotope discrimination. The CER9 protein thus encodes a novel determinant of plant drought tolerance-associated traits, one whose deficiency elevates cutin synthesis, redistributes wax composition, and suppresses transpiration. Map-based cloning identified CER9, and sequence analysis predicted that it encodes an E3 ubiquitin ligase homologous to yeast Doa10 (previously shown to target endoplasmic reticulum proteins for proteasomal degradation). To further elucidate CER9 function, the impact of CER9 deficiency on interactions with other genes was examined using double mutant and transcriptome analyses. For both wax and cutin, cer9 showed mostly additive effects with cer6, long-chain acyl-CoA synthetase1 (lacs1), and lacs2 and revealed its role in early steps of both wax and cutin synthetic pathways. Transcriptome analysis revealed that the cer9 mutation affected diverse cellular processes, with primary impact on genes associated with diverse stress responses. The discovery of CER9 lays new groundwork for developing novel cuticle-based strategies for improving the drought tolerance and water use efficiency of crop plants.
Selenium Distribution and Speciation in the Hyperaccumulator Astragalus bisulcatus and Associated Ecological Partners
Authors: Valdez Barillas José R. , Quinn Colin F. , Freeman John L. , Lindblom Stormy D. , Fakra Sirine C. , Marcus Matthew A. , Gilligan Todd M. , Alford Élan R. , Wangeline Ami L. , Pilon-Smits Elizabeth A.H. 
Published: May 2012

Abstract
The goal of this study was to investigate how plant selenium (Se) hyperaccumulation may affect ecological interactions and whether associated partners may affect Se hyperaccumulation. The Se hyperaccumulator Astragalus bisulcatus was collected in its natural seleniferous habitat, and x-ray fluorescence mapping and x-ray absorption near-edge structure spectroscopy were used to characterize Se distribution and speciation in all organs as well as in encountered microbial symbionts and herbivores. Se was present at high levels (704–4,661 mg kg−1 dry weight) in all organs, mainly as organic C-Se-C compounds (i.e. Se bonded to two carbon atoms, e.g. methylselenocysteine). In nodule, root, and stem, up to 34% of Se was found as elemental Se, which was potentially due to microbial activity. In addition to a nitrogen-fixing symbiont, the plants harbored an endophytic fungus that produced elemental Se. Furthermore, two Se-resistant herbivorous moths were discovered on A. bisulcatus, one of which was parasitized by a wasp. Adult moths, larvae, and wasps all accumulated predominantly C-Se-C compounds. In conclusion, hyperaccumulators live in association with a variety of Se-resistant ecological partners. Among these partners, microbial endosymbionts may affect Se speciation in hyperaccumulators. Hyperaccumulators have been shown earlier to negatively affect Se-sensitive ecological partners while apparently offering a niche for Se-resistant partners. Through their positive and negative effects on different ecological partners, hyperaccumulators may influence species composition and Se cycling in seleniferous ecosystems.
Tioguanine in the treatment of refractory coeliac disease – a single centre experience
Authors: Tack G. J. , van Asseldonk D. P. , van Wanrooij R. L. J. , van Bodegraven A. A. , Mulder C. J. 
Published: May 2012

SummaryBackgroundRefractory coeliac disease type I is a complicated form of coeliac disease characterised by primary or secondary resistance to a gluten‐free diet with persisting or reoccurring intestinal villous atrophy and symptoms of malabsorption. Besides corticosteroids, azathioprine has been advocated for the treatment of refractory coeliac disease type I. However, tioguanine (TG) might be better tolerated and more efficacious owing to a simpler metabolism towards bioactivation.AimTo evaluate tolerability and effectiveness of the nonconventional thiopurine derivative TG in refractory coeliac disease type I.MethodsRefractory coeliac disease type I patients treated with TG between June 2001 and November 2010 with a follow‐up period of at least 1 year were included. Adverse events, laboratory values, 6‐thioguanine nucleotide concentrations and rates of both clinical and histological response were evaluated at baseline and during follow‐up.ResultsTwelve adult refractory coeliac disease type I patients were included. The median TG treatment duration was 14 months. Ten patients tolerated TG treatment on the long term, whereas two patients withdrew treatment due to adverse events. No nodular regenerative hyperplasia of the liver was observed. During follow‐up clinical and histological response was observed in 83% and 78%, respectively. Corticosteroid dependency decreased by 50%.ConclusionTioguanine appears to be a convenient drug for the treatment of refractory coeliac disease type I based on higher histological and similar clinical response rates as compared with historical conventional therapies.
Factors associated with family reunification for children in foster care
Authors: López Mónica , del Valle Jorge F. , Montserrat Carme , Bravo Amaia 
Published: May 2012

ABSTRACTIn this paper, we analyse reunification processes from family foster care, both kinship and non‐kinship, and the variables associated with them in a Spanish sample. Data collection was carried out after a review of child protection and foster care files, and those responsible for the cases were also consulted. The cases studied were closed, which made it possible to assess the final outcome of the fostering. We found that only one‐fifth of the children return to their birth family after the fostering, and that those in non‐kinship are less likely to do so. Seven factors were associated with family reunification processes: age of the child, kinship care with family co‐operation, drug dependence in parents, neglect due to alcoholism, foster care under voluntary arrangement with visits, short‐term fostering with family co‐operation and parental impossibility due to imprisonment. The contribution of these data takes on particular importance given that the Spanish child protection system keeps no official statistics on the development or outcome of its interventions and records even less information on the factors associated with such outcomes.
Practitioner accounts of responding to parent abuse – a case study in ad hoc delivery, perverse outcomes and a policy silence
Authors: Holt Amanda , Retford Simon 
Published: May 2012

ABSTRACTParent abuse is becoming recognized as a serious problem in some families. It can have a damaging impact on physical and mental health, family relationships and employment and has been found to be implicated in other past, current and future forms of family abuse and violence. For this reason, many frontline practitioners who work with troubled families frequently find incidents of parent abuse in their caseloads, but we know little of how they respond to it. This study used in‐depth interviews with nine practitioners who work in a range of agencies in one large county in England and explored how they each identify, conceptualize, explain and respond to parent abuse. In a context where there is no national guidance regarding how agencies should respond to this problem, this study finds that practitioners must ‘make do’ without appropriate resources or policy guidance to help them. The study concludes with suggestions for change for the benefit of families who seek support but who currently find little effective response.
Supporting families living with parental substance misuse: the M‐PACT (Moving Parents and Children Together) programme
Authors: Templeton Lorna 
Published: May 2012

ABSTRACTThe Moving Parents and Children Together (M‐PACT) programme is one of the growing number of interventions tailored to meet the multiple and complex needs of children and families affected by parental substance misuse. This paper pulls together the qualitative findings from 13 evaluated M‐PACT programmes in England. Sixty‐four families attended an M‐PACT programme, including 82 children and 75 adults. Qualitative data were collected from 37 children, 36 adults and over 30 group facilitators. Six themes are discussed: engaging with M‐PACT, shared experiences, understanding addiction, changes in communication, healthier and united families, and ending M‐PACT. The majority of families benefitted in a range of ways from the programme: meeting others who were experiencing similar problems, greater understanding about addiction and its impact on children and families improving communication within the family. In many families there was more openness and honesty, stronger relationships and more time as families, and a reduction in arguments and conflict. The key findings are discussed in terms of the potential for interventions of this kind to reduce family‐related harm from parental substance misuse.
Attachment and adaptive skills in children of international adoption
Authors: Barcons Natàlia , Abrines Neus , Brun Carme , Sartini Claudio , Fumadó Victoria , Marre Diana 
Published: May 2012

ABSTRACTThe attachment pattern of a sample of 168 internationally adopted children was explored in this study using the semi‐structured Friends and Family Interview. The pattern was analysed in relation to the development of adaptive skills as an expression of the children's resilience.The secure attachment pattern rates were slightly lower and the insecure attachment patterns were considerably higher than those of normative samples from the previous studies. The children from Eastern Europe demonstrated a more insecure attachment pattern (odds ratio [OR]= 2.46; confidence interval [CI]= 1.23–3.94), and their scores on the adaptive skills scales were lower than the scores of children from other countries (OR = 2.62; 95% CI = 1.02–6.72). These results help to identify the groups at risk of failing to develop secure attachment patterns and appropriate adaptive skills, and should provide valuable information for designing effective interventions.
Is interferon-gamma the right marker for bacille Calmette–Guérin-induced immune protection? The missing link in our understanding of tuberculosis immunology
Authors: Abebe F 
Published: May 2012

SummaryBacille Calmette–Guérin (BCG), developed a century ago, is the only licensed tuberculosis (TB) vaccine in use to date. The protective efficacy of BCG against TB varies with no apparent protection in some population, and mechanisms of its immune protection is poorly known, and yet BCG is the most widely used vaccine, with more than 4 billion BCG-vaccinated children globally. BCG is probably the only licensed vaccine currently in use believed to mediate immune protection through the production of interferon (IFN)-γ by CD4 T cells, which in turn activates macrophages to kill Mycobacterium tuberculosis (Mtb). Currently, a number of new TB candidate vaccines are in different phases of clinical trial. The majority of these new vaccines are either recombinant forms of BCG or prime boosters of BCG (rBCG) and their immunogenicity is tested using BCG as a benchmark by measuring specific IFN-γ produced by CD4+ T cells as a protective immune marker. However, some recent studies that examined mechanisms of immune protection of BCG in animals and humans have reported a lack of correlation between IFN-γ production by CD4 cells and BCG-induced immune protection. These studies point to the fact that there is a missing link in our understanding of TB immunology. Conversely, there is emerging evidence that other T cell subsets (gammadelta, γδ), CD8+ T cells and natural killer (NK) cells may play a vital role in immune protection against Mtb infection and BCG-induced immune protection. γδ T cells and NK cells, which were considered to be part of the innate immunity in the past, have been shown to develop immunological memory upon re-encounter with the same pathogen. In this paper, the controversy over the role of IFN-γ as a marker for protective immunity against TB, and emerging data on the role of γδ T cells, CD8+ and NK cells in TB immunology, will be presented.
Rapid cardiovascular effects of growth hormone treatment in short prepubertal children: impact of treatment duration
Authors: Nygren Anders , Sunnegårdh Jan , Teien Dag , Jonzon Anders , Björkhem Gudrun , Lindell Sven , Albertsson‐Wikland Kerstin , Kriström Berit 
Published: May 2012

SummaryObjectivePrevious studies show that growth hormone (GH) treatment increases cardiac dimensions in short children with GH deficiency (GHD) and has diverse cardiac effects in children with idiopathic short stature (ISS). This study was performed to assess the effect of GH on the cardiovascular system in short children with a broad range of GH secretion and GH sensitivity/responsiveness.Design and patientsIn this prospective, multicentre study, short prepubertal children diagnosed with isolated GHD (89) or ISS (38) were followed during 2 years of GH treatment. They were randomized to receive either a standard (43 μg/kg/day) or an individualized GH dose (range 17–100 μg/kg/day) based on GH responsiveness estimated by a prediction model and distance to target height. Echocardiography, blood pressure and electrocardiography were performed at baseline, 3, 12 and 24 months.ResultsLeft ventricular mass (LVM) indexed to body surface area increased significantly during 2 years of GH treatment in both GHD and ISS irrespective of randomized dose. This change was already apparent at 3 months, when standard deviation scores (SDS) of wall thickness and diameter were increased. At 24 months, left ventricular diameter SDS remained increased, whereas myocardial thickness SDS returned to baseline values. There was no impairment of systolic or diastolic function. There was no correlation with treatment dose and LVM SDS at 24 months.ConclusionsIrrespective of GH status, there was a rapid increase in LVM during GH treatment in short children. At 3 months, wall thickness and diameter were increased, whereas only diameter remained increased at 24 months.