bluuebunny/crossref_metadata_2025_split huggingface dataset:
HAX1 deficiency: Impact on lymphopoiesis and B‐cell development
Authors: Peckl‐Schmid Doris , Wolkerstorfer Susanne , Königsberger Sebastian , Achatz‐Straussberger Gertrude , Feichtner Stefan , Schwaiger Elisabeth , Zaborsky Nadja , Huemer Michael , Gratz Iris K. , Schibli Roger , Lamers Marinus , Crameri Reto , Moser Katrin , Luger Elke O , Achatz Gernot 
Published: August 2010

AbstractHAX1 was originally described as HS1‐associated protein with a suggested function in receptor‐mediated apoptotic and proliferative responses of lymphoid cells. Recent publications refer to a complex and multifunctional role of this protein. To investigate the in vivo function of HAX1 (HS1‐associated protein X1) in B cells, we generated a Hax1‐deficient mouse strain. Targeted deletion of Hax1 resulted in premature death around the age of 12 wk accompanied by a severe reduction of lymphocytes in spleen, thymus and bone marrow. In the bone marrow, all B‐cell populations were lost comparably. In the spleen, B220+ cells were reduced by almost 70%. However, as investigated by adoptive transfer experiments, this impairment is not exclusively B‐cell intrinsic and we hypothesize that a HAX1‐deficient environment cannot sufficiently provide the essential factors for proper lymphocyte development, trafficking and survival. Hax1−/− B cells show a significantly reduced expression of CXCR4, which might have an influence on the observed defects in B‐cell development.
5‐(2‐Thienyl)tetrazolates as Ligands for RuII–Polypyridyl Complexes: Synthesis, Electrochemistry and Photophysical Properties
Authors: Stagni Stefano , Palazzi Antonio , Brulatti Pierpaolo , Salmi Mauro , Muzzioli Sara , Zacchini Stefano , Marcaccio Massimo , Paolucci Francesco 
Published: August 2010

AbstractIn this contribution, we report the synthesis, the characterization and the study of the electrochemical and photophysical properties of some novel mononuclear RuII–polypyridyl complexes of the general formula [Ru(tpy)(bpy)L]+ (tpy = 2,2′:6′,2"‐terpyridine, bpy = 2,2′‐bipyridyl) in which L is represented by 5‐(2‐thienyl)tetrazolate‐type ligands. In addition, the reactivity of these mononuclear species toward different electrophiles such as H+ and CH3+ has been investigated, and the effects of the resulting regioselective electrophilic attacks on the electronic and structural properties of the tetrazolate ligand have been studied by NMR (1H, 13C) spectroscopy and X‐ray crystal structural determination. Also, the electronic properties of the complexes have been explored by means of absorption and emission spectroscopy, together with a detailed electrochemical investigation combined with density functional theory (DFT) calculations. The data obtained from the study of such “model” mononuclear species constituted the basis for the characterization of the corresponding bithienyl‐bridged dinuclear RuII complex, which displayed similar physical–chemical properties and an analogous behaviour toward the addition of electrophilic agents.
Proteomic profiling of human colon cancer cells treated with the histone deacetylase inhibitor belinostat
Authors: Beck Hans Christian , Petersen Jørgen , Nielsen Søren Jensby , Morszeck Christian , Jensen Peter B. , Sehested Maxwell , Grauslund Morten 
Published: August 2010

AbstractThe anticancer drug belinostat is a hydroxamate histone deacetylase inhibitor that has shown significant antitumour activity in various tumour models and also in clinical trials. In this study, we utilized a proteomic approach in order to evaluate the effect of this drug on protein expression in the human colon cancer cell line HCT116. Protein extracts from untreated HCT116 cells, and cells grown for 24 h in the presence of 1 and 10 μM belinostat were analysed by 2‐D gel electrophoresis. Proteins were visualized by colloidal Coomassie blue staining and quantitative analysis of gel images revealed 45 unique differentially expressed proteins that were identified by LC‐MSMS analysis. Among these proteins, of particular interest are the downregulated proteins nucleophosmin and stratifin, and the upregulated proteins nucleolin, gelsolin, heterogeneous nuclear ribonucleoprotein K, annexin 1, and HSP90B that all were related to the proto‐oncogene proteins p53, Myc, activator protein 1, and c‐fos protein. The modulation of these proteins is consistent with the observations that belinostat is able to inhibit clonogenic cell growth of HCT116 cells and the biological role of these proteins will be discussed.
Effect of Ethane and Propane in Simulated Natural Gas on the Operation of Ni–YSZ Anode Supported Solid Oxide Fuel Cells
Authors: Bierschenk D. M. , Pillai M. R. , Lin Y. , Barnett S. A. 
Published: August 2010

AbstractSolid oxide fuel cells with Ni–yittria‐stabilised zirconia (YSZ) anode supports were tested on surrogate natural gas fuels (methane containing 2.5–10% ethane and 1.25–5% propane) and compared with results for pure methane. Inert anode‐side diffusion barriers were found to help suppress coking on the Ni–YSZ anodes. However, carbonaceous deposits were observed on anode compartment surfaces and the barrier layers for all of the natural gas compositions tested. The addition of air to the natural gas was shown to suppress this coking. For natural gas with 5% ethane and 2.5% propane, the addition of 33% air yielded stable, coke‐free operation at 750 °C and 800 mA cm–2. Cell performance on this fuel was only slightly worse than for the same cell operated with dry hydrogen.
Development of Proton Conducting SOFCs Based on LaNbO4 Electrolyte – Status in Norway
Authors: Magrasó A. , Fontaine M.‐L. , Larring Y. , Bredesen R. , Syvertsen G. E. , Lein H. L. , Grande T. , Huse M. , Strandbakke R. , Haugsrud R. , Norby T. 
Published: August 2010

AbstractHigh temperature proton conducting solid oxide fuel cells (PC‐SOFCs) are in a developing state. Electrolytes in these cells should exhibit proton conductivity with essentially no electronic and little other ionic conductivity, as well as long‐term stability in acidic atmospheres. Acceptor substituted rare‐earth ortho‐niobates and ortho‐tantalates were recently demonstrated to exhibit proton conductivity in wet atmospheres, with a maximum of ∼10–3 S cm–1 for 1% Ca‐doped LaNbO4. This modest proton conductivity requires that the electrolyte thickness is in the micron range to reach acceptable PC‐SOFC performances. The long‐term chemical stability and a proton transference number close to unity make these materials highly interesting for high temperature fuel cell applications, in contrast to the more investigated acceptor‐doped BaCeO3 that shows instability towards acidic atmospheres. Here, we describe collaborative efforts between Norwegian partners: SINTEF, Norwegian University of Science and Technology (NTNU) and the University of Oslo for developments towards a fuel cell based on LaNbO4. This comprises identification of materials for the electrodes, interconnect and sealing, optimisation of the microstructures of all cell components, development of shaping processes and design of the fuel cell stack. We address the crucial technological issues of building and testing a PC‐SOFC stack, as well as the comprehensive fundamental understanding of all the processes involved – from fabrication and behaviour of individual components to fabrication of PC‐SOFC fuel cell stacks.
African and non‐African admixture components in African Americans and an African Caribbean population
Authors: Murray Tanda , Beaty Terri H. , Mathias Rasika A. , Rafaels Nicholas , Grant Audrey Virginia , Faruque Mezbah U. , Watson Harold R. , Ruczinski Ingo , Dunston Georgia M. , Barnes Kathleen C. 
Published: August 2010

AbstractAdmixture is a potential source of confounding in genetic association studies, so it becomes important to detect and estimate admixture in a sample of unrelated individuals. Populations of African descent in the US and the Caribbean share similar historical backgrounds but the distributions of African admixture may differ. We selected 416 ancestry informative markers (AIMs) to estimate and compare admixture proportions using STRUCTURE in 906 unrelated African Americans (AAs) and 294 Barbadians (ACs) from a study of asthma. This analysis showed AAs on average were 72.5% African, 19.6% European and 8% Asian, while ACs were 77.4% African, 15.9% European, and 6.7% Asian which were significantly different. A principal components analysis based on these AIMs yielded one primary eigenvector that explained 54.04% of the variation and captured a gradient from West African to European admixture. This principal component was highly correlated with African vs. European ancestry as estimated by STRUCTURE (r2=0.992, r2=0.912, respectively). To investigate other African contributions to African American and Barbadian admixture, we performed PCA on ∼14,000 (14k) genome‐wide SNPs in AAs, ACs, Yorubans, Luhya and Maasai African groups, and estimated genetic distances (FST). We found AAs and ACs were closest genetically (FST=0.008), and both were closer to the Yorubans than the other East African populations. In our sample of individuals of African descent, ∼400 well‐defined AIMs were just as good for detecting substructure as ∼14,000 random SNPs drawn from a genome‐wide panel of markers. Genet. Epidemiol. 34:561–568, 2010.© 2010 Wiley‐Liss, Inc.
Synthesis and characterization of chroman‐containing compounds and their preliminary assessment of cytotoxicity toward two human cancer cell lines
Authors: Tang Ying , Wei Jie , Zhong Wei , Liu Xiaoming 
Published: August 2010

AbstractA series of chroman derivatives were synthesized by employing o‐methyl phenol as a precursor. These compounds were fully characterized using IR, NMR spectroscopic techniques, and elemental analysis. Mechanisms for the formation of the chroman moiety of these compounds were also proposed. MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay was used to estimate their cytotoxicity toward two cancer cell lines: human ovarian cancer (A2780) and human cervical cancer (Hela). © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:423–429, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20621
Elucidation of the stereochemistry of thiirane formation from a 1λ4,2‐dithietane bearing two chiral carbon centers
Authors: Kusaka Shinpei , Kano Naokazu , Kawashima Takayuki 
Published: August 2010

AbstractA tetracoordinated sulfurane bearing a 1λ4,2‐dithietane moiety, whose 3‐ and 4‐positions were chiral carbon centers, was synthesized. Its thermolysis gave the corresponding thiirane as a single diastereomer, whose configuration and stereochemistry were determined. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:412–417, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20630
Synthesis of new 5‐acetyl(arylmethyliden)‐4‐thiazolidones
Authors: Polovkovych Svyatoslav V. , Karkhut Andrii I. , Marintsova Natalia G. , Novikov Volodymyr P. 
Published: August 2010

AbstractA new approach to the synthesis of new heterocyclic compounds with triazine and 4‐thiazolidone fragments in one molecule is developed. The synthesis methods comprise [2+3]‐cyclocondensation reactions essential in the preparative synthesis of 4‐thiazolidone derivatives. The reactions of S,N‐nucleophiles with C2‐cyclization agents for the synthesis of a number of biologically active 2‐triazin‐4‐thiazolidones were investigated. The interaction of thiosemicarbazone of sym‐triazine with derivatives of α‐halogencarboxylic acids and maleic anhydride resulted in correspondent (2‐[2‐(4,6‐dichloro‐1,3,5‐triazin‐2‐yl)hydrazino]‐5‐(3,4,5‐ R‐p‐phenyl‐methyliden)‐1,3‐thiazol‐4‐ones obtained in the one‐step synthesis. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:392–396, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20631
O‐2′,3′‐Ketal‐Nucleolipids of the Cytostatic 5‐Fluorouridine: Synthesis, Lipophilicity, and Acidic Stability
Authors: Malecki Edith , Rosemeyer Helmut 
Published: August 2010

AbstractThe synthesis of a series of cyclic and acyclic O‐2′,3′‐ketal derivatives of the cancerostatic 5‐fluorouridine (2a) is described. The novel compounds were characterized by 1H‐ and 13C‐NMR, and UV spectroscopy, as well as by elemental analyses. The lipophilicity values (log P, retention times in RP‐18 HPLC) of the cyclic ketals were determined and related to the ring tensions as well as the acid stability of the spiro‐linked ketal rings.
Data hiding in halftone images with secret‐shared dot diffusion
Authors: Guo Jing‐Ming , Huang Jyun‐Hao 
Published: August 2010

AbstractThis study presents a low‐complexity watermarking for embedding a watermark into two or more halftone images with dot diffusion. The first halftone image is obtained by traditional dot diffusion, while the others are achieved by applying the proposed secret‐shared dot diffusion (SSDDF) technique. The visual decoding pattern can be observed when these similar dot‐diffused images are printed on transparencies and then overlaid each other. An improved decoded result can be yielded by computer‐aid XNOR operation. The proposed scheme is proved to be superior to the former dot diffusion with nonlinear thresholding (DDNT) proposed by Taheri et al. from the conducted experiments. Moreover, two extensions, namely dual SSDDF (DSSDDF) and adaptive SSDDF (ASSDDF), are also proposed to provide better decoding rates and compromised image quality. © 2010 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 20, 294‐300, 2010
Multiview foreground extraction and composition to multiview background using trimap sharing for natural 3D scene generation
Authors: Hyun Myung‐Han , Kim Sung‐Yeol , Kang Yun‐Suk , Ho Yo‐Sung 
Published: August 2010

AbstractDigital matting for extracting foreground objects from an image is an important process to generate special effects in the movie industry and the broadcasting center. Recently, a digital matting algorithm has been developed to create an alpha matte using a well‐focused image generated from multiview images. However, this method could generate only a single‐view alpha matte, even though it used multiple cameras. In this article, we propose a new estimation scheme for multiview alpha mattes by sharing the trimap of the reference view. Furthermore, we use the motion vector to update the trimap for video matting. After we extract foreground objects from all view images, we composite the foreground objects with the corresponding background images captured in the same multiview camera arrangement. Experimental results demonstrate that multiview composite images can generate reasonably natural 3D views through the stereoscopic monitor. © 2010 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 20, 285‐293, 2010
VEGF stimulation enhances livin protein synthesis through mTOR signaling
Authors: Yan Biao , Kong Men , Chen Shi , Chen Yi‐han 
Published: August 2010

AbstractLivin is a member of inhibitors of apoptosis proteins (IAPs) and overexpressed in transformed cells and several cancers. Although strategies to decrease Livin levels have been conducted for rational cancer therapy, the molecular mechanism controlling Livin expression in tumors has not been completely elucidated. Here, we show that vascular endothelial growth factor (VEGF) stimulation can increase Livin expression in HeLa cells or SK‐MEL‐28 cells. This response is independent of de novo gene transcription or changes in mRNA expression but occurs at protein expression levels. VEGF stimulation results in mTOR signaling activation which changes the phosphorylation status of 4E‐BP1, the downstream of mTOR signaling, and ultimately contributes to the translation initiation of Livin protein. Livin silencing, Rapamycin alone or in combination with cytotoxic agent can reduce Livin protein levels, and decrease cells viability. Thus, ablation of Livin translation contributes to remove an anti‐apoptotic mechanism potentially contributing to aggressive tumor behavior. Pharmacologic inhibition of VEGF/mTOR/Livin signaling may provide a novel strategy for cancer treatment. J. Cell. Biochem. 111: 1114–1124, 2010. © 2010 Wiley‐Liss, Inc.
Lysyl oxidase propeptide sensitizes pancreatic and breast cancer cells to doxorubicin‐induced apoptosis
Authors: Min Chengyin , Zhao Yingshe , Romagnoli Mathilde , Trackman Philip C. , Sonenshein Gail E. , Kirsch Kathrin H. 
Published: August 2010

AbstractRAS mutations or its activation by upstream receptor tyrosine kinases are frequently associated with poor response of carcinomas to chemotherapy. The 18 kDa propeptide domain of lysyl oxidase (LOX‐PP) released from the secreted precursor protein (Pro‐LOX) has been shown to inhibit RAS signaling and the transformed phenotype of breast, pancreatic, lung, and prostate cancer cells in culture, and formation of tumors by Her‐2/neu‐driven breast cancer cells in a mouse xenograft model. Here, we tested the effects of LOX‐PP on MIA PaCa‐2 pancreatic cancer cells, driven by mutant RAS. In MIA PaCa‐2 cells in culture, LOX‐PP attenuated the ERK and AKT activities and decreased the levels of the NF‐κB p65 and RelB subunits and cyclin D1, which are activated by RAS signaling. In mouse xenograft growth, LOX‐PP reduced growth of tumors by these pancreatic cancer cells, and the nuclear levels of the p65 NF‐κB subunit and cyclin D1 proteins. While biological agents attenuate tumor growth when used alone, often they have additive or synergistic effects when used in combination with chemotherapeutic agents. Thus, we next tested the hypotheses that LOX‐PP sensitizes pancreatic and breast cancer cells to the chemotherapeutic agent doxorubicin. Purified LOX‐PP enhanced the cytotoxic effects of doxorubicin in pancreatic and breast cancer cells, as judged by ATP production, Cell Death ELISA assays, caspase 3 activation, PARP cleavage, and Annexin V staining. Thus, LOX‐PP potentiates the cytotoxicity of doxorubicin on breast and pancreatic cancer cells, warranting additional studies with a broader spectrum of current cancer treatment modalities. J. Cell. Biochem. 111: 1160–1168, 2010. © 2010 Wiley‐Liss, Inc.
Protein kinases and ovarian functions
Authors: Sirotkin Alexander V. , Makarevich Alexander V. , Grosmann Roland 
Published: August 2010

AbstractThe present focus survey represents a short review of current knowledge concerning involvement of protein kinases in control of basic ovarian functions. Ovarian cells produce a number of protein kinases, whose expression depends on type of cells, their state and action of hormones and other protein kinases. A number of protein kinases are involved in control of ovarian cell proliferation, apoptosis, oocyte maturation, hormone release, reception and response to hormones, as well as in mediating action of hormones on these ovarian functions. Complexity of interrelationships between different protein kinase‐dependent signaling pathways occurs. Protein kinases and their regulators could be used for characterization, prediction and control of ovarian folliculogenesis and atresia, Corpus luteum functions, oocyte maturation, fertility, release of hormones, response of ovarian structures to hormonal regulators, as well as for treatment of some reproductive disorders. The present data demonstrate importance of protein kinases in control of basic ovarian function and potential usage of protein kinases for characterization, prediction and control of these functions. J. Cell. Physiol. 226: 37–45, 2010. © 2010 Wiley‐Liss, Inc.
Interaction of profilin‐1 and F‐actin via a β‐arrestin‐1/JNK signaling pathway involved in prostaglandin E2‐induced human mesenchymal stem cells migration and proliferation
Authors: Yun Seung Pil , Ryu Jung Min , Jang Min Woo , Han Ho Jae 
Published: August 2010

AbstractAlthough many previous reports have examined the function of prostaglandin E2 (PGE2) in the migration and proliferation of various cell types, the role of the actin cytoskeleton in human mesenchymal stem cells (hMSCs) migration and proliferation has not been reported. The present study examined the involvement of profilin‐1 (Pfn‐1) and filamentous‐actin (F‐actin) in PGE2‐induced hMSC migration and proliferation and its related signal pathways. PGE2 (10−6 M) increased both cell migration and proliferation, and also increased E‐type prostaglandin receptor 2 (EP2) mRNA expression, β‐arrestin‐1 phosphorylation, and c‐Jun N‐terminal kinase (JNK) phosphorylation. Small interfering RNA (siRNA)‐mediated knockdown of β‐arrestin‐1 and JNK (‐1, ‐2, ‐3) inhibited PGE2‐induced growth of hMSCs. PGE2 also activated Pfn‐1, which was blocked by JNK siRNA, and induced F‐actin level and organization. Downregulation of Pfn‐1 by siRNA decreased the level and organization of F‐actin. In addition, specific siRNA for TRIO and F‐actin‐binding protein (TRIOBP) reduced the PGE2‐induced increase in hMSC migration and proliferation. Together, these experimental data demonstrate that PGE2 partially stimulates hMSCs migration and proliferation by interaction of Pfn‐1 and F‐actin via EP2 receptor‐dependent β‐arrestin‐1/JNK signaling pathways. J. Cell. Physiol. 226: 559–571, 2011. © 2010 Wiley‐Liss, Inc.
Asymmetrical cell division and differentiation are not dependent upon stratification in a corneal epithelial cell line
Authors: Gómez‐Flores Eber , Sánchez‐Guzmán Erika , Castro‐Muñozledo Federico 
Published: August 2010

AbstractTo determine whether asymmetrical cell division takes place during growth and differentiation of corneal epithelial cells, we analyzed the expression of some proteins required for the correct execution of the asymmetric division in cultured RCE1‐(5T5) cells, which mimic the differentiation of corneal epithelial cells. RT‐PCR and immunostaining showed that Par‐3, LGN (GPSM2), NuMA, and the mammalian homolog of inscuteable (Insc) are expressed by the cultured cells. Semi‐quantitative RT‐PCR demonstrated that Insc mRNA levels were stable throughout the experiment. Conversely, LGN and NuMA mRNAs increased slightly and steadily in proliferative cells, reaching a peak of about 20% above basal levels when cells were confluent. At later times, LGN and NuMA mRNAs decreased to become barely detectable when cells organized into a four‐layered epithelium and expressed terminal phenotype as indicated by the highest expression of LDH‐H mRNA. Cultivation under low Ca2+ conditions (0.09 mM) reduced about 50% Insc mRNA expression both in proliferating and confluent cultures, but did not affect the levels of LGN and NuMA mRNAs. Hence, asymmetric cell division seems to take place with a lower frequency in cells grown with low Ca2+ concentrations, in spite of the absence of stratification. Immunostaining experiments raise the possibility of an interaction between k3/K12 keratin cytoskeleton and Par‐3. The results show for the first time the coordination between the expression of corneal epithelial cell differentiation and the expression of cell polarity machinery. They also suggest that asymmetric division does not depend on stratification; instead, it seems to be part of the differentiation program. J. Cell. Physiol. 226: 700–709, 2011. © 2010 Wiley‐Liss, Inc.