bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leukemic mutations in the methylation‐associated genes DNMT3A and IDH2 are rare events in pediatric AML: A report from the Children's Oncology Group
Authors: Ho Phoenix A. , Kutny Matthew A. , Alonzo Todd A. , Gerbing Robert B. , Joaquin Jason , Raimondi Susana C. , Gamis Alan S. , Meshinchi Soheil 
Published: April 2011

AbstractBackgroundMutations in the DNMT3A, TET2, IDH1, and IDH2 genes carry prognostic significance and occur frequently in adult acute myeloid leukemia (AML). Leukemic mutations in all four genes have recently been implicated in aberrant DNA methylation, a hallmark of neoplasia. We previously reported that IDH1 mutations were absent, whereas TET2 mutations were present in 6%, of pediatric AML patients; in the present study, we determined the prevalence of DNMT3A and IDH2 mutations in pediatric AML.MethodsWe screened for DNMT3A and IDH2 mutations by direct sequencing of diagnostic specimens from 180 children treated on the Children's Oncology Group clinical trial AAML03P1. Clinical characteristics, the presence of other leukemic mutations, and survival outcome was determined for mutation‐positive patients.ResultsNo disease‐associated DNMT3A mutations were detected. IDH2 mutations were detected in 4/180 patients (2.2%), affecting codons R140 (n = 3) and R172 (n = 1). Two patients with IDH2 mutations harbored t(8;21), one patient harbored an MLL translocation, and one patient had a concomitant NPM1 mutation. FLT3, CEBPA, and WT1 mutations did not occur together with IDH2 mutations in our study.ConclusionDNMT3A and IDH2 mutations are uncommon in pediatric AML. The low prevalence of methylation‐associated mutations in our study highlights the differences in the pathogenesis of pediatric versus adult AML, at the genetic as well as potentially at the epigenetic level. The age‐specific characteristics of AML underscore the importance of studying the molecular biology of both childhood and adult forms of this leukemia in parallel, as the development of novel therapeutics should account for these biologic differences. Pediatr Blood Cancer 2011; 57: 204–209. © 2011 Wiley‐Liss, Inc.
Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach
Authors: He Dongli , Han Chao , Yao Jialing , Shen Shihua , Yang Pingfang 
Published: April 2011

AbstractConstruction of metabolic and regulatory pathways from proteomic data can contextualize the large‐scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1‐DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.
Using 2‐DE for the differentiation of local chicken breeds
Authors: Zanetti Enrico , Molette Caroline , Chambon Christophe , Pinguet Jeremy , Rémignon Hervé , Cassandro Martino 
Published: April 2011

AbstractThe aim of this study was to apply a proteomic approach for the characterisation of local chicken breeds. The experiment involved a total of 29 males of three local Italian chicken breeds: Pépoi, Padovana and Ermellinata di Rovigo. Sarcoplasmic protein fractions of breast muscle were analysed by 2‐DE. Image analysis followed by statistical analysis enabled to differentiate groups of individuals based on the similarities of protein expression. Individuals were distinguished into clusters and groups, corresponding to the breed of origin. Distances among individuals, calculated using data on spot volumes, were used to draw a neighbour‐joining tree, showing clear individual and breed grouping. The most relevant spots regarding breed differentiation were detected; 11 were identified by MS revealing preliminary evidences on the mechanisms of the breed differentiation process. The results evidenced the ability of proteomic analyses for the characterisation of chicken breeds.
ProteoConnections: A bioinformatics platform to facilitate proteome and phosphoproteome analyses
Authors: Courcelles Mathieu , Lemieux Sébastien , Voisin Laure , Meloche Sylvain , Thibault Pierre 
Published: April 2011

AbstractNovel and improved computational tools are required to transform large‐scale proteomics data into valuable information of biological relevance. To this end, we developed ProteoConnections, a bioinformatics platform tailored to address the pressing needs of proteomics analyses. The primary focus of this platform is to organize peptide and protein identifications, evaluate the quality of the acquired data set, profile abundance changes, and accelerate data interpretation. Peptide and protein identifications are stored into a relational database to facilitate data mining and to evaluate the quality of data sets using graphical reports. We integrated databases of known PTMs and other bioinformatics tools to facilitate the analysis of phosphoproteomics data sets and to provide insights for subsequent biological validation experiments. Phosphorylation sites are also annotated according to kinase consensus motifs, contextual environment, protein domains, binding motifs, and evolutionary conservation across different species. The practical application of ProteoConnections is further demonstrated for the analysis of the phosphoproteomics data sets from rat intestinal IEC‐6 cells where we identified 9615 phosphorylation sites on 2108 phosphoproteins. Combined proteomics and bioinformatics analyses revealed valuable biological insights on the regulation of phosphoprotein functions via the introduction of new binding sites on scaffold proteins or the modulation of protein–protein, protein–DNA, or protein–RNA interactions. Quantitative proteomics data can be integrated into ProteoConnections to determine the changes in protein phosphorylation under different cell stimulation conditions or kinase inhibitors, as demonstrated here for the MEK inhibitor PD184352.
Mechanistic study on Mo(CO)6‐catalyzed intramolecular [2 + 2] or [2 + 2 + 1] cycloaddition reaction of 5‐allenyl‐1‐ynes
Authors: Meng Qingxi , Li Ming 
Published: April 2011

AbstractBy means of density functional theory, the Mo(CO)6‐catalyzed intramolecular [2 + 2] or [2 + 2 + 1] cycloaddition reaction of 5‐allenyl‐1‐ynes was investigated. All the intermediates and transition states were optimized completely at B3LYP/6‐311++G(d,p) level (LANL2DZ(f) for Mo). Calculations indicate that the complexation of 5‐allenyl‐1‐ynes with Mo(CO)6 occurred preferentially at the triple bond to give the complex M1 and then the complexation with the distal double bond of the allenes generates the complex M5. In this reaction, Mo(CO)6‐catalyzed intramolecular [2 + 2] cycloaddition is more favorable than [2 + 2 + 1] cycloaddition. The reaction pathway Mo(CO)6 + R → M5 → T7 → M12 → M13 → T11 → M18 → P4 is the most favorable one, and the most dominant product predicted theoretically is P4. The solvation effect is remarkable, and it decreases the reaction energy barriers. Copyright © 2011 John Wiley & Sons, Ltd.
High‐pressure phase transitions in NaBH4 from first‐principles calculations
Authors: Zhu Chunye , Liu Yanhui , Tian Fubo , Cui Tian 
Published: April 2011

AbstractWe present a study on NaBH4 under high pressure by using the ab initio pseudopotential plane‐wave method. The calculations show that a structural phase transition takes place at 10.91 GPa from the β‐NaBH4 structure (tetragonal‐$P{\bar {4}}{\rm 2}_{{\rm 1}} c$) to the γ‐NaBH4 structure (orthorhombic‐Pnma). Band‐structure calculation reveals that the two phases show an insulator behaviour with a finite energy gap. There is no pressure‐induced softening behaviour from our calculated phonon dispersion curves near the phase transition pressure. We also characterize charge transfer and Mulliken population analyses of these structures. The Mulliken population analyses indicate that the β‐NaBH4 phase is expected to be the most promising candidate for hydrogen storage.
High‐pressure Raman study on CeO2 nanospheres self‐assembled by 5 nm CeO2 nanoparticles
Authors: Liu Bo , Liu Bingbing , Li Quanjun , Li Zepeng , Yao Mingguang , Liu Ran , Zou Xu , Lv Hang , Wu Wei , Cui Wen , Liu Zhaodong , Li Dongmei , Zou Bo , Cui Tian , Zou Guangtian 
Published: April 2011

AbstractCeO2 undergoes a first‐order phase transition from fluorite to α‐PbCl2‐type structure under high pressure. To evaluate the changes in physical properties of CeO2 nanomaterials as the particle size decreasing, high‐pressure Raman study under quasi‐hydrostatic condition has been performed on CeO2 nanospheres self‐assembled by 5 nm CeO2 nanoparticles at room temperature. Surprisingly, as the pressure elevate to 34 GPa, the CeO2 nanospheres still retain the cubic fluorite‐type structure, indicating the sample is more stable than the bulk counterpart. Whereas, previous high‐pressure studies show the phase transition at 22.3/26.5 GPa for 12 nm CeO2 nanoparticles, which is less stable than the bulk materials. The enhancement of phase stability might be attributed to the increase of surface energy of CeO2 nanospheres as the size of the building units decrease.
Rayleigh–ritz method for excited quantum states via nonlinear variations without constraints: Role of supersymmetry
Authors: Mukherjee Neetik , Bhattacharyya Kamal 
Published: April 2011

AbstractQuantum mechanical variation principle in the form of energy minimization is applicable only to ground states of systems, or, at best, states of lowest energies of given symmetries, provided the symmetry information is embedded in chosen trial functions. Thus, for bound quantum states with specified choices of trial functions involving nonlinear parameters, scope of the principle is severely restricted. A pedagogic way out is to enforce exact orthogonality of the chosen function with all exact lower energy states. In actual practice, this limits one to opt for linear variations where upper bound to each state is obtained in a single run. In this work, the motivation is to explore if there exists at all a way to determine optimized wave functions and energies for excited states via nonlinear variations but without any constraints, even for simple systems. Realizing that the major problem in excited‐state nonlinear variations is concerned with the variations of nodal positions, at least for problems reducible to one dimension, we seek a route via which nodes could be fixed beforehand, so that the information gained may be subsequently utilized to construct a suitable nonlinear trial function and carry out a straightforward optimization. To achieve this end, the idea of supersymmetric quantum mechanics has been used quite profitably, yielding the nodal structure of the excited states. Workability of the strategy for several excited‐state wave functions and their properties is demonstrated by choosing the problems of spherical Stark effect on hydrogen atom and anharmonic oscillator. © 2011 Wiley Periodicals, Inc. Int J Quantum Chem, 2012
Theoretical study on the reaction of the phenoxy radical with O2, OH, and NO2
Authors: Batiha Marwan , Al‐Muhtaseb Ala'a H. , Altarawneh Mohammednoor 
Published: April 2011

AbstractUnlike the chemistry underlying the self‐coupling of phenoxy (C6H5O) radicals, there are very limited kinetics data at elevated temperatures for the reaction of the phenoxy radical with other species. In this study, we investigate the addition reactions of O2, OH, and NO2 to the phenoxy radical. The formation of a phenoxy‐peroxy is found to be very slow with a rate constant fitted to k = 1.31 × 10−20T2.49 exp (−9300/T) cm3/mol/s in the temperature range of (298–2,000 K) where the addition occurs predominantly at the ortho site. Our rate constant is in line with the consensus of opinions in the literature pointing to the observation of no discernible reaction between the oxygen molecule and the resonance‐stabilized phenoxy radical. Addition of OH at the ortho and para sites of the phenoxy radical is found to afford adducts with sizable well depths of 59.8 and 56.0 kcal/mol, respectively. The phenoxy‐NO2 bonds are found to be among the weakest known phenoxy‐radical bonds (1.7–8.7 kcal/mol). OH‐ and O2‐initiated mechanisms for the degradation of atmospheric phenoxy appear to be negligible and the fate of atmospheric phenoxy is found to be controlled by its reaction with NO2. © 2011 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
A theoretical study on the reaction mechanisms of O(3P)+1‐butene
Authors: Zhao Hongmei , Pan Lu , Bian Wensheng 
Published: April 2011

AbstractThe reaction of O(3P) with 1‐butene (CH3CH2CHCH2) are examined by applying the UMP2 and G3 methods. The minimum energy crossing points (MECPs) between the singlet and triplet potential energy surfaces are located using the Newton‐Lagrange method, and it is shown that the MECPs play a key role in the reaction mechanisms. The complex reaction mechanisms are revealed for both adiabatic and nonadiabatic reaction channels, and the observations in several recent experiments can be rationalized based upon the present calculations. The calculational results indicate that the site selectivity of the addition of O(3P) to either carbon atom of the double bond of 1‐butene is not remarkable. In addition, the formation mechanisms of butenols are discussed. The butenols can be created not only by the keto‐enol tautomerization, but also by the rearrangement and decomposition reaction involving the epoxy compound. © 2011 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
Mixtures of multiple testing procedures for gatekeeping applications in clinical trials
Authors: Dmitrienko Alex , Tamhane Ajit C. 
Published: April 2011

AbstractThis paper proposes a general framework for constructing gatekeeping procedures for clinical trials with hierarchical objectives. Such problems frequently exhibit complex structures including multiple families of hypotheses and logical restrictions. The proposed framework is based on combining multiple procedures across families. It enables the construction of powerful and flexible gatekeeping procedures that account for general logical restrictions among the hypotheses of interest. A clinical trial in patients with schizophrenia is used to illustrate the approach for parallel gatekeeping, whereas another clinical trial in patients with hypertension is used to illustrate the approach for gatekeeping with general logical restrictions. Copyright © 2011 John Wiley & Sons, Ltd.
Cutoff designs for community‐based intervention studies
Authors: Pennell, Michael L. , Hade, Erinn M. , Murray David M. , Rhoda Dale A. 
Published: April 2011

AbstractPublic health interventions are often designed to target communities defined either geographically (e.g. cities, counties) or socially (e.g. schools or workplaces). The group randomized trial (GRT) is regarded as the gold standard for evaluating these interventions. However, community leaders may object to randomization as some groups may be denied a potentially beneficial intervention. Under a regression discontinuity design (RDD), individuals may be assigned to treatment based on the levels of a pretest measure, thereby allowing those most in need of the treatment to receive it. In this article, we consider analysis, power, and sample size issues in applying the RDD and related cutoff designs in community‐based intervention studies. We examine the power of these designs as a function of intraclass correlation, number of groups, and number of members per group and compare results to the traditional GRT. Copyright © 2011 John Wiley & Sons, Ltd.
Numerical analysis
Authors: Brandimarte Paolo 
Published: April 2011

AbstractIn this article, we touch on a few selected topics illustrating the interplay between computational statistics and numerical analysis. Rather than briefly reviewing a long array of methods, on one hand we place emphasis on problems, such as stochastic optimization, which require the joint application of several techniques; on the other hand, we stress a few recurring themes such as conditioning, numerical stability, convergence, and different discretization approaches that are at the root of many numerical methods. Then, we examine some forms of function approximation, the solution of algebraic and functional equations, and numerical quadrature. In particular, we outline some possibly less familiar topics, such as homotopy continuation and collocation methods.WIREs Comp Stat2011 3 434–449 DOI: 10.1002/wics.172This article is categorized under:Algorithms and Computational Methods > Dynamic ProgrammingStatistical and Graphical Methods of Data Analysis > Markov Chain Monte Carlo (MCMC)Algorithms and Computational Methods > Numerical Methods
Breast and Ovarian Cancer: The Forgotten Paternal Contribution
Authors: McCuaig Jeanna M. , Greenwood Celia M. T. , Shuman Cheryl , Chitayat David , Murphy K. Joan , Rosen Barry , Armel Susan Randall 
Published: April 2011

AbstractFive to 10% of all cases of breast and ovarian cancer are attributed to a heritable genetic predisposition. Transmission of BRCA1 and BRCA2 mutations is equally likely through maternal or paternal lineage; however, fewer referrals to cancer genetics clinics appear to be made for a paternal, than maternal, family history of breast and/or ovarian cancer. To examine this potential bias, a retrospective review of 315 patient and family charts was conducted by one familial cancer clinic in Toronto, Canada. Referral letters, risk estimates, and family histories were analyzed to identify significant differences between patients referred with maternal and paternal family histories. It was determined that patients are approximately five times more likely to be referred with a maternal family history of breast and/or ovarian cancer as compared to those with a paternal family history (p = <.0001). Individuals with a paternal family history were found to have a different, and higher, pattern of risk estimates (p = .00064). No significant difference was seen between the type of referrals sent by general practitioners, oncologists, and gynecologists. Recommendations to increase the awareness of paternal family history in assessing cancer risk are provided.
Effect of Dehulling Treatment on the Oxidative Stability of Cold‐Pressed Low Erucic Acid Rapeseed Oil
Authors: Yang Mei , Liu Changsheng , Huang Fenghong , Zheng Chang , Zhou Qi 
Published: April 2011

AbstractThis study compared the oxidative stability of cold‐pressed rapeseed oil (CPRO) and dehulled cold‐pressed rapeseed oil (DCPRO) in the dark at 60 °C and monitored the evolution of minor constituents (tocopherols, phytosterols, phenolics). The results showed that dehulling significantly influenced the oxidative stability of the oils, the DCPRO was more easily oxidized. During the autoxidation, the peroxide value (PV) and anisidine value (p‐AV) of the DCPRO ranged from 2.38 to 95.97 mequiv O2 kg−1 and from 1.20 to 30.75, whereas those of the CPRO ranged from 3.80 to 46.17 mequiv O2 kg−1and from 2.69 to 14.87, respectively. Dehulling affected the contents and the rates of decrease of tocopherols and phytosterols of the cold‐pressed oils, and the rates of decrease of tocopherols and phytosterols of the CPRO were lower than those of the DCPRO (10% less, on average). The rancimat induction periods (IPs) were positively correlated with the concentrations of the total tocopherols (For DCPRO, R2 = 0.9622, For CPRO, R2 = 0.8334). The total phenolics contents as determined by spectrophotometry first increased and then decreased. Tocopherols and phytosterols had a greater effect on oxidative stability of the rapeseed oils during the first 30 days, and phenolics had a greater effect in the 30–40 day period.
Determinants of perinatal mortality in Nigeria
Authors: Fawole Adeniran O. , Shah Archana , Tongo Olukemi , Dara Kabir , El‐Ladan Aliyu M. , Umezulike Augustine C. , Alu Frank E. , Eniayewun Ademuyiwa B. , Fabanwo Adetokunbo O. , Adewunmi Adeniyi A. , Adegbola Omololu , Adebayo Amos A. , Obaitan Felix O. , Onala Olanrewaju E. , Usman Yalwa , Sullayman Abdulkareem O. , Kailani Sikiratu , Sa'id Mohammed 
Published: April 2011

AbstractObjectiveTo determine risk factors for perinatal mortality among hospital‐based deliveries in Nigeria.MethodsThe WHO Global Maternal and Perinatal Health Survey was implemented in Nigeria as a first step in establishing a global system for monitoring maternal and perinatal health. Twenty‐one health facilities with more than 1000 deliveries annually were selected by a stratified multistage cluster sampling strategy. Information was recorded on all women who delivered and their neonates within a 3‐month period.ResultsOverall, there were 9208 deliveries, comprising 8526 live births, 369 fresh stillbirths, 282 macerated stillbirths, 70 early neonatal deaths, and 721 perinatal deaths. The stillbirth and perinatal mortality rates were, respectively, 71 and 78 per 1000 deliveries; the early neonatal death rate was 8 per 1000 live births. Approximately 10% of all newborns weighed less than 2500 g, and 12.3% were born at less than 37 weeks of gestation. Predictors of perinatal mortality were mother's age, lack of prenatal care, unbooked status, prematurity, and birth asphyxia.ConclusionThe perinatal mortality rate remains unacceptably high in Nigeria. Fresh stillbirth accounted for most perinatal deaths. Interventions to improve the utilization and quality of prenatal care, in addition to the quality of intrapartum care, would considerably reduce perinatal death.
Epidemiologie - was fällt auf? Unterschiede in der Gesundheit von Frauen und Männern
Authors: Hagen Christine , Starker Anne 
Published: April 2011

Einleitung
Das Geschlecht gilt als zentrale Kategorie, die Gesundheit und Krankheit, das gesundheitsrelevante Verhalten sowie die Inanspruchnahme von Versorgungsleistungen beeinflusst. Der Beitrag gibt einen Überblick über die geschlechtsspezifischen Unterschiede hinsichtlich Lebenserwartung, Mortalität und Morbidität und verweist darauf, dass diese in erster Linie auf gesundheitsbezogene Verhaltensunterschiede sowie unterschiedliche Lebensbedingungen zwischen Männern und Frauen zurückzuführen sind.
Asymmetrien in den Gesundheitsberufen: Männer- oder Frauendömane?
Authors: Pundt Johanne , Drees Lena 
Published: April 2011

Einleitung
Wie zeigen sich die geschlechtsspezifischen Berufsbilder in der Gesundheitsbranche - eine der bedeutendsten Wirtschaftszweige mit 4,6 Mio. Mitarbeitern - ? Was hat sich unter dem geschlechtsspezifischen Blickwinkel in den letzten Jahren entwickelt, welche Trends existieren und wo gibt es dringenden Handlungsbedarf? Bei aller Diskussion um Gender Mainstreaming und Geschlechtersensibilisierung steht fest, dass das Gesundheitswesen personell betrachtet nach wie vor eine weibliche Domäne ist. Die typischen Frauenberufe besetzen hier 74%, die Präsenz innerhalb der Gesundheitsdienstberufe liegt sogar bei 80% und hat sich im Vergleich zur Gesamtwirtschaft fast verdoppelt.