bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of conservation agriculture on soil C and N stocks and organic matter fractions: comparing commercial producer fields with a long-term small-plot experiment in Brown Chernozems of Saskatchewan
Authors: St. Luce Mervin , McConkey Brian , Schoenau Jeff , Brandt Kelsey , Hangs Ryan , Zhang Hongjie 
Published: May 2024

Conservation agriculture (CA) is increasingly promoted to build soil organic matter (SOM) based on findings from predominantly small-plot long-term agroecosystem experiments (LTAEs), with minimal on-farm data. Using commercial producer fields ( n = 20) in the Brown Chernozemic soil zones of Saskatchewan, Canada, which were sampled before (1996) and after (2018) adopting direct-seeding and continuous cropping (1997), we examined changes in soil organic carbon (SOC) and soil total nitrogen (STN) stocks, along with C and N stocks in particulate (POM) and mineral-associated organic matter (MAOM), and compared them to an LTAE in the same soil zone. After 21 years, SOC and STN stocks (0–30 cm depth) increased by 13% and 21%, respectively, in commercial producer fields, and were more pronounced in finer- than coarser-textured soils. Conversely, there were no significant changes (0–30 cm depth) after 18 years (1998–2016) with CA (continuous wheat and pulse-wheat under no-tillage (PW-NT)) in the LTAE, except that STN stock for PW-NT decreased by 7.7%. The estimated rate of change to 30 cm depth was similar between the commercial fields and LTAE for SOC (0.28 and 0.16 Mg C ha−1 year−1, respectively), but not STN (0.04 and −0.03 Mg N ha−1 year−1, respectively). Changes were more evident in the MAOM than POM fraction in both cases. Although the impact of CA may be similar, as observed for SOC, actual on-farm changes will depend on site-specific factors, and specific CA practice. Therefore, on-farm monitoring studies are needed for more accurate assessments of SOM changes and C sequestration potentials.
Droplet motion driven by humidity gradients during evaporation and condensation
Authors: Barrio-Zhang Hernán , Ruiz-Gutiérrez Élfego , Orejon Daniel , Wells Gary G. , Ledesma-Aguilar Rodrigo 
Published: May 2024

Abstract
The motion of droplets on solid surfaces in response to an external gradient is a fundamental problem with a broad range of applications, including water harvesting, heat exchange, mixing and printing. Here we study the motion of droplets driven by a humidity gradient, i.e. a variation in concentration of their own vapour in the surrounding gas phase. Using lattice-Boltzmann simulations of a diffuse-interface hydrodynamic model to account for the liquid and gas phases, we demonstrate that the droplet migrates towards the region of higher vapour concentration. This effect holds in situations where the ambient gradient drives either the evaporation or the condensation of the droplet, or both simultaneously. We identify two main mechanisms responsible for the observed motion: a difference in surface wettability, which we measure in terms of the Young stress, and a variation in surface tension, which drives a Marangoni flow. Our results are relevant in advancing our knowledge of the interplay between gas and liquid phases out of thermodynamic equilibrium, as well as for applications involving the control of droplet motion.

Graphic abstract
Adverse Childhood Experiences, Resilience, and Cardiovascular Disease in Adult Survivors of Childhood Cancer: A Report from the Childhood Cancer Survivor Study
Authors: Schwartz Lindsay F. , Stratton Kayla L. , Leisenring Wendy M. , Rodriguez Stefania M. , Alston Shani , McDonald Aaron , Vukadinovich Chris , Rinehardt Dayton , Oeffinger Kevin C. , Chow Eric J. , Krull Kevin R. , Brinkman Tara M. , Nathan Paul C. , Tan Marcia M. , McCrae Julie S. , Burkhardt Tiffany , Ness Kirsten K. , Armstrong Gregory T. , Henderson Tara O. 
Published: May 2024

Abstract

Background:
The impact of adverse childhood experiences (ACE, e.g., abuse, neglect, and/or household dysfunction experienced before the age of 18) and resilience on risk for cardiovascular disease (CVD) has not previously been investigated in adult survivors of childhood cancer.

Methods:
We conducted a nested case–control study among long-term, adult-aged survivors of childhood cancer from the Childhood Cancer Survivor Study. Self-report questionnaires ascertained ACEs and resilience, and scores were compared between cases with serious/life-threatening CVD and controls without CVD matched on demographic and cardiotoxic treatment factors.

Results:
Among 95 cases and 261 controls, the mean ACE score was 1.4 for both groups; 53.4% of survivors endorsed ≥1 ACE. No association was observed between ACEs or resilience and CVD in adjusted models.

Conclusions:
ACEs and resilience do not appear to contribute to CVD risk for adult survivors of childhood cancer with cardiotoxic treatment exposures.

Impact:
Although not associated with CVD in this population, ACEs are associated with serious health issues in other populations. Therefore, future studies could investigate the effects of ACEs on other health outcomes affecting childhood cancer survivors.
SLC22A17 as a Cell Death–Linked Regulator of Tight Junctions in Cerebral Ischemia
Authors: Li Wenlu , Shi Jingfei , Yu Zhanyang , Garcia-Gabilondo Miguel , Held Aaron , Huang Lena , Deng Wenjun , Ning Mingming , Ji Xunming , Rosell Anna , Wainger Brian J. , Lo Eng H. 
Published: May 2024

BACKGROUND:
Beyond neuronal injury, cell death pathways may also contribute to vascular injury after stroke. We examined protein networks linked to major cell death pathways and identified SLC22A17 (solute carrier family 22 member 17) as a novel mediator that regulates endothelial tight junctions after ischemia and inflammatory stress.

METHODS:
Protein-protein interactions and brain enrichment analyses were performed using STRING, Cytoscape, and a human tissue-specific expression RNA-seq database. In vivo experiments were performed using mouse models of transient focal cerebral ischemia. Human stroke brain tissues were used to detect SLC22A17 by immunostaining. In vitro experiments were performed using human brain endothelial cultures subjected to inflammatory stress. Immunostaining and Western blot were used to assess responses in SLC22A17 and endothelial tight junctional proteins. Water content, dextran permeability, and electrical resistance assays were used to assess edema and blood-brain barrier (BBB) integrity. Gain and loss-of-function studies were performed using lentiviral overexpression of SLC22A17 or short interfering RNA against SLC22A17, respectively.

RESULTS:
Protein-protein interaction analysis showed that core proteins from apoptosis, necroptosis, ferroptosis, and autophagy cell death pathways were closely linked. Among the 20 proteins identified in the network, the iron-handling solute carrier SLC22A17 emerged as the mediator enriched in the brain. After cerebral ischemia in vivo, endothelial expression of SLC22A17 increases in both human and mouse brains along with BBB leakage. In human brain endothelial cultures, short interfering RNA against SLC22A17 prevents TNF-α (tumor necrosis factor alpha)–induced ferroptosis and downregulation in tight junction proteins and disruption in transcellular permeability. Notably, SLC22A17 could repress the transcription of tight junctional genes. Finally, short interfering RNA against SLC22A17 ameliorates BBB leakage in a mouse model of focal cerebral ischemia.

CONCLUSIONS:
Using a combination of cell culture, human stroke samples, and mouse models, our data suggest that SLC22A17 may play a role in the control of BBB function after cerebral ischemia. These findings may offer a novel mechanism and target for ameliorating BBB injury and edema after stroke.
The Brain's Sensitivity to Real-world Statistical Regularity Does Not Require Full Attention
Authors: Center Evan G. , Federmeier Kara D. , Beck Diane M. 
Published: May 2024

Abstract
Predictive coding accounts of perception state that the brain generates perceptual predictions in the service of processing incoming sensory data. These predictions are hypothesized to be afforded by the brain's ability to internalize useful patterns, that is, statistical regularities, from the environment. We have previously argued that the N300 ERP component serves as an index of the brain's use of representations of (real-world) statistical regularities. However, we do not yet know whether overt attention is necessary in order for this process to engage. We addressed this question by presenting stimuli of either high or low real-world statistical regularity in terms of their representativeness (good/bad exemplars of natural scene categories) to participants who either fully attended the stimuli or were distracted by another task (attended/distracted conditions). Replicating past work, N300 responses were larger to bad than to good scene exemplars, and furthermore, we demonstrate minimal impacts of distraction on N300 effects. Thus, it seems that overtly focused attention is not required to maintain the brain's sensitivity to real-world statistical regularity. Furthermore, in an exploratory analysis, we showed that providing additional, artificial regularities, formed by altering the proportions of good and bad exemplars within blocks, further enhanced the N300 effect in both attended and distracted conditions, shedding light on the relationship between statistical regularities learned in the real world and those learned within the context of an experiment.
Paradoxical Long-term Memory Augmentation following Temporal Pairing between “Limited” and “Extensive” Motor Sequence Training Experiences
Authors: Oren Yaniv , Korman Maria , Karni Avi 
Published: May 2024

Abstract
Consecutive training on two movement sequences often leads to retroactive interference—obstructing memory for the initially trained sequence but not for the second. However, in the context of hippocampal-system dependent memories, a poor learning experience, memory for which would soon decay, can be enhanced if temporally paired with a “strong” memory triggering experience. The synaptic tagging and capture hypothesis explains this paradoxical enhancement by suggesting that only strong experiences generate cellular resources necessary for synaptic remodeling. However, synapses engaged in a “weak” learning experience can capture and utilize plasticity-related resources generated for a subsequent strong learning experience. Here, we tested whether such a “paradoxical” outcome would result in the context of motor (procedural) memory, if two movement sequences are unequally trained, consecutively. We show, in young adults (n = 100), that limited practice on a novel sequence of finger-to-thumb opposition movements led to different long-term outcomes, depending on whether and when (5 min, 5 hr) it was followed by extensive training on a different sequence. Five-minute pairing only resulted in overnight gains for the limited-trained sequence that were well-retained a week later; the overnight gains for the extensively trained sequence were compromised. Thus, consecutive training on different motor tasks can result in mnemonic interactions other than interference. We propose that the newly discovered mnemonic interaction provides the first-tier behavioral evidence in support of the possible applicability of notions stemming from the synaptic tagging and capture hypothesis in relation to human motor memory generation, specifically in relation to the practice-dependent consolidation of novel explicitly instructed movement sequences.
Cross-sectional and Longitudinal Age-related Disintegration in Functional Connectivity: Reference Ability Neural Network Cohort
Authors: Argiris Georgette , Stern Yaakov , Habeck Christian 
Published: May 2024

Abstract
Some theories of aging have linked age-related cognitive decline to a reduction in distinctiveness of neural processing. Observed age-related correlation increases among disparate cognitive tasks have supported the dedifferentiation hypothesis. We previously showed cross-sectional evidence for age-related correlation decreases instead, supporting an alternative disintegration hypothesis. In the current study, we extended our previous research to a longitudinal sample. We tested 135 participants (20–80 years) at two time points—baseline and 5-year follow-up—on a battery of 12 in-scanner tests, each tapping one of four reference abilities. We performed between-tasks correlations within domain (convergent) and between domain (discriminant) at both the behavioral and neural level, calculating a single measure of construct validity (convergent − discriminant). Cross-sectionally, behavioral construct validity was significantly different from chance at each time point, but longitudinal change was not significant. Analysis by median age split revealed that older adults showed higher behavioral validity, driven by higher discriminant validity (lower between-tasks correlations). Participant-level neural validity decreased over time, with convergent validity consistently greater than discriminant validity; this finding was also observed at the cross-sectional level. In addition, a disproportionate decrease in neural validity with age remained significant after controlling for demographic factors. Factors predicting longitudinal changes in global cognition (mean performance across all 12 tasks) included age, change in neural validity, education, and National Adult Reading Test (premorbid intelligence). Change in neural validity partially mediated the effect of age on change in global cognition. Our findings support the theory of age-related disintegration, linking cognitive decline to changes in neural representations over time.
Real-Time Use of SGLT2i Verified in Pre-dialysis: The RSVP Cross-sectional Study
Authors: Wisbaum Aylon , Gaudreau Sandrine , Cloutier Isabelle , Robert Pascale , Kolment Regina , Beauchesne Marie-France , Couture Jodianne 
Published: May 2024

Background: The use of sodium-glucose cotransporter 2 inhibitors (SGLT2i) in nephrology practice is increasingly becoming standard of care in patients with diabetes or those with proteinuria. Objectives: The primary outcome was to identify the proportion of pre-dialysis patients with chronic kidney disease (CKD) G3a, G3b, or G4 prescribed an SGLT2i and describe their characteristics. Methods: This was a retrospective, multicentric, cross-sectional study of patients with CKD followed at 4 pre-dialysis clinics in the province of Quebec, Canada. We collected data of multiple covariates associated with prescribing SGLT2i in patients over 18 years of age with CKD G3a, G3b, or G4. We then performed a multivariate logistic regression to assess their associations. Results: Of the 874 patients included, 22.7% were prescribed an SGLT2i. Factors most strongly associated included male sex (odds ratio [OR] = 4.88, 95% CI = 2.38-10.03), being prescribed metformin (OR = 4.30, 95% CI = 2.23-8.31), having type 2 diabetes (OR = 4.00, 95% CI = 1.86-8.62), or having an albumin-to-creatinine ratio greater than 300 mg/g (OR = 1.84, 95% CI = 1.08-3.14). The majority of patients (60.4%) had their SGLT2i initiated by the pre-dialysis clinic and the most frequent adverse event was an initial increase in serum creatinine 1 week after starting treatment (33.9%). Conclusion and relevance: An increasing number of patients with CKD are being prescribed SGLT2i. Nonetheless, significant disparities in sex, severity of disease, and comorbidities remain. We suggest that specific strategies be put in place to promote prescribing of SGLT2i in women and other at-risk populations, in particular among nephrology teams, to improve patient care.
Evaluation of a Medical Fitness Referral Program at a Single Institution From 2015 to 2022
Authors: Lu Zhen Arthur , Ziegler Craig , Tharp Elise , Cole Darby , Price James 
Published: May 2024

Purpose: To assess whether biometric data of participants (i.e., weight, body mass index (BMI), body fat percentage (BFP), heart rate, and blood pressure) would be statistically different from pre- to post-program assessments after completion of a single institution medical fitness referral program. Methods: This is a retrospective analysis of randomly selected participants from August 2015 to December 2022. Analysis of variance was performed for each outcome with groups separated by gender, age, and time period relative to COVID. Results: Analysis included 984 participants, 653 (66%) were female, mean age was 55.5 years (SD = 14.9) with 297 (30%) of participants ≥65, and 747 (76%) completed the program prior to COVID. Significant mean reductions from pre- to post-program were seen for BMI (−.43, SD = 1.16), weight (-2.69 pounds, SD = 7.44), BFP (.44%, SD = 2.35%), systolic blood pressure (3.33 mmHg, SD = 14.58), and diastolic blood pressure (2.18 mmHg, SD = 8.65). Additional significant interactions identified: weight difference for participants less than age 65 (−3.16, SD = 8.31) vs participants ≥65 (−1.60, SD = 4.66), P = .003. Conclusions: In this analysis, participants of the program from August 2015 to December 2022 were seen to have statistically significant reductions in the delineated biometric parameters. However, due to limitations, specific conclusions regarding causation cannot be drawn.
TEVAR with fenestrations of all supra-aortic branches for traumatic aortic pseudoaneurysm: Case report and systematic review
Authors: Wang Yashi , Wang Bing , Qiu Chenyang 
Published: May 2024

Objectives Blunt thoracic aortic injuries (BTAIs) involving the aortic arch are a challenging condition. Thoracic endovascular aortic repair (TEVAR) with fenestration, which expands the proximal landing zone, is able to exclude the injury while preserving blood flow in supra-aortic branches. Methods Here we report a case of TEVAR with fenestrations of all supra-aortic branches for traumatic aortic pseudoaneurysm and perform a systematic review. Results A 24-year-old man suffering a blunt thoracic injury and a left femoral fracture was sent to our hospital. A pseudoaneurysm was found in the aortic arch between the brachiocephalic artery and the left common carotid artery. The patient underwent emergent TEVAR with fenestrations of all supra-aortic branches, which excluded the pseudoaneurysm and preserved the patency of all branches. The orthopedic team then treated the femoral fracture. The patient’s recovery was unremarkable. We performed a systematic review on TEVAR with fenestrations for BTAI. Six patients (75%) received TEVAR with single fenestration, 1 patient (12.5%) received TEVAR with two fenestrations, and 1 patient (12.5%) had fenestrations of all supra-aortic branches. Except one patient died in the perioperative, other patients survived without stent-related complications in the short-term follow-up. Conclusions TEVAR with fenestration is feasible for treating BTAI involving the aortic arch in selected patients.
Outcomes of Blood Flow Restriction Training After ACL Reconstruction in NCAA Division I Athletes
Authors: Devana Sai K. , Solorzano Carlos A. , Vail Jeremy , Jackson Nicholas , Pham Derek , Jones Kristofer J. 
Published: May 2024

Background: Blood flow restriction training (BFRT) is a safe and potentially effective adjunctive therapeutic modality for postoperative rehabilitation related to various knee pathologies. However, there is a paucity of literature surrounding BFRT in high-performance athletes after anterior cruciate ligament reconstruction (ACLR). Purpose: To (1) compare the overall time to return to sports (RTS) in a cohort of National Collegiate Athletic Association (NCAA) Division I athletes who underwent a standardized rehabilitation program either with or without BFRT after ACLR and (2) identify a postoperative time interval for which BFRT has the maximum therapeutic benefit. Study Design: Cohort study; Level of evidence, 3. Methods: A total of 55 student-athletes who underwent ACLR between 2000 and 2023 while participating in NCAA Division I sports at a single institution were included in this study. Athletes were allocated to 1 of 2 groups based on whether they participated in a standardized postoperative rehabilitation program augmented with BFRT (BFRT group; n = 22) or completed the standardized protocol alone (non-BFRT group [control]; n = 33). Our primary outcome measure was time to RTS. The secondary outcome measure was handheld dynamometry quadriceps strength testing at various postoperative time points, converted to a limb symmetry index (LSI). Quadriceps strength was not tested between the BFRT and non-BFRT groups because of the limited amount of data on the control group. Results: The mean age at the date of surgery was 18.59 ± 1.10 years for the BFRT group and 19.45 ± 1.30 years for the non-BFRT group ( P = .011), and the mean RTS time was 409 ± 134 days from surgery for the BFRT group and 332 ± 100 days for the non-BFRT cohort ( P = .047). For the BFRT group, the mean quadriceps strength LSI increased by 0.67% (95% CI, 0.53%-0.81%) for every week of rehabilitation, and there was a significantly positive rate of change in quadriceps strength in weeks 13-16 compared with weeks 9-12 (ΔLSI, 8.22%; P < .001). Conclusion: In elite NCAA Division I athletes, a statistically significant delay was observed in RTS with BFRT compared with standardized physical therapy alone after undergoing ACLR. There also appeared to be an early window during the rehabilitation period where BFRT had a beneficial impact on quadriceps strength.