bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential catalytic action of Brevibacillus laterosporus on two dissimilar azo dyes Remazol red and Rubine GFL
Authors: Kurade Mayur B. , Waghmode Tatoba R. , Tamboli Dhawal P. , Govindwar Sanjay P. 
Published: May 2012

AbstractThis comparative study disclosed the diverse catalytic activities of Brevibacillus laterosporus on two different azo dyes. It decolorized 100% of Remazol red and 95% of Rubine GFL within 30 and 48 h respectively, under static condition at 50 mg l−1 dye concentration. Significant increase was observed in azo reductase, NADH‐DCIP reductase, veratryl alcohol oxidase and tyrosinase in cells obtained after decolorization of Remazol red; whereas these values were much different with complete inhibition of azo reductase during decolorization of Rubine GFL. The plausible pathway of dye degradation obtained from Gas chromatography‐Mass spectroscopy (GC‐MS) data confirmed the different metabolic fate of these structurally unidentical dyes. FTIR and HPTLC analysis of extracted metabolites confirmed the biodegradation, while phytotoxicity study assured the detoxification of both the dyes studied. The results obtained in this study suggests, i) sulpho and hydroxyl group present at ortho position to azo group stimulated reduction of azo bond by azo reductase in Remazol red, ii) the same reduction was totally hampered due to presence of ethyl‐amino propanenitrile group at para position to azo group in Rubine GFL.
Molecular epidemiology of carbapenem‐resistant Acinetobacter spp. from XiangYa Hospital, in Hunan Province, China
Authors: Chen Zhenhua , Liu Wenen , Zhang Yunli , Li Yanbing , Jian Zijuan , Deng Hongli , Zou Mingxiang , Liu Yuanyuan 
Published: May 2012

AbstractCarbapenem‐resistant Acinetobacter baumannii has been known as an opportunistic pathogen that causes a variety of illness worldwide. In this study, we characterize the molecular epidemiology of 174 non‐repetitive clinical isolates of A. baumannii collected from January to June 2009 from Xiangya Hospital, in Hunan Province, China, including an outbreak period of A. baumannii. These 174 isolates harbored A. baumannii intrinsic gene OXA‐51. They were resistant to multiple antibiotics with resistance rates as 49.4% to imipenem, 48.3% to meropenem, 46.6% to ampicillin‐sulbactam, 6.9% to cefoperazone‐sulbactam, and 6.3% to minocycline. 74 out of 174 isolates were identified as carbapenemase‐producing strains, among which blaOXA‐23 gene was found in 71 isolates. These 74 carbapenemase expression strains could be divided into four genotypes by enterobacterial repetitive intergenic consensus (ERIC)‐PCR, with 19, 17, 33 and 5 clones in each group. We also found four imipenem resistant isolates carrying OXA‐23 gene without showing carbapenemase phenotype. Our findings show that the blaOXA‐23 gene is the common carbapenemase gene among carbapenem‐resistant Acinetobacter spp. isolates, suggesting that clonal spread of carbapenemase‐producing isolates may be one important factor which results in the high carbapenem resistance rate in the local hospital in Hunan Province, China.
The expression of LytM is down‐regulated by RNAIII in Staphylococcus aureus
Authors: Chunhua Mu , Yu Liu , Yaping Gao , Jie Dong , Qiang Lu , Xiaorong Tan , Guang Yang 
Published: May 2012

AbstractLytM is one kind of autolysin expressed in Staphylococcus aureus. It can hydrolyze peptide bond of pentaglycine, which is the component of cell wall. Until now, the regulation on the expression of LytM is still unclear. Here, we found that the level of LytM in the RNAIII mutant was increased. RNAIII is an important small regulatory RNA in S. aureus. Our further investigation revealed that RNAIII can interact with the 5′UTR of lytM mRNA and block the ribosome binding sites [RBS]. So our results identified that LytM was the new target of RNAIII. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Glucose released by hydrolytic activity of amylase influences the pigment synthesis in Penicillium sp NIOM‐02
Authors: Puttananjaiah Mohan‐Kumari H. , Dhale Mohan A. 
Published: May 2012

AbstractCarbon catabolite repression is generally considered as a regulatory mechanism to ensure sequential synthesis of secondary metabolites. In this study we made an attempt to understand the influence of amylase activity on pigment synthesis in Penicillium sp NIOM‐02. The amylase activity is inversely proportional to pigment production. The high performance liquid chromatography analysis of amylase reaction revealed glucose as the major product of starch hydrolysis. The fungus grown in acarbose (inhibitor of amylase) incorporated media produced higher quantities of pigments. Apparently, glucose released due to amylase activity influenced the pigment synthesis by cAMP signaling pathway.
Genotypic intraspecies heterogeneity of Enterococcus italicus: data from dairy environments
Authors: Borgo Francesca , Ferrario Chiara , Ricci Giovanni , Fortina Maria Grazia 
Published: May 2012

AbstractThe diversity of a collection of 19 Enterococcus italicus strains isolated from different dairy sources was explored using a molecular polyphasic approach, comprising random amplification of polymorphic DNA (RAPD‐PCR), repetitive element PCR (REP‐PCR), plasmid profiling and ribotyping. The data obtained showed a high‐level of biodiversity, not always correlated to the niche of isolation. Particularly, REP‐PCR with primer BOXA1R and plasmid profiling allowed the best discrimination at strain level. Exploiting the genome shotgun sequence of the type strain of the species, available in public database, genes related to insertion sequences present on enterococcal Pathogenic Islands (ISEf1, IS905), determinants related to virulence factors (codifying for hemolysin and cell wall surface proteins), exogenously DNA (conjugal transfer protein, replication plasmid protein, pheromone shutdown protein, phage integrase/recombinase) and penicillin binding proteins system were detected. The presence of most of these genes seemed a common genetic trait in the Enterococcus genus, sur gene (cell wall surface protein) was only detected in strains of E. italicus. To our knowledge, this is the first time that specific primers, with the expection of the species‐specific probe targeted to 16S rRNA gene, have been designed for this species.
Characterization of the microbial diversity in production waters of mesothermic and geothermic Tunisian oilfields
Authors: Mnif Sami , Bru‐Adan Valérie , Godon Jean‐Jacques , Sayadi Sami , Chamkha Mohamed 
Published: May 2012

AbstractThe microbial diversity of production waters of five Tunisian oilfields was investigated using Single Strand Conformation Polymorphism (SSCP) technique followed by cloning‐sequencing. Dynamics of bacterial populations in production waters collected from four wellheads were also evaluated. For all production water samples collected, DNA from Archaea and Eucarya was not sufficiently abundant to permit detection rRNA genes from these groups by PCR‐SSCP. In contrast, the bacterial rRNA genes were detected in all samples, except for samples from DOULEB12 and RAMOURA wells. SSCP profiles attested that two of the studied geothermic wells (ASHTART47 and ASHTART48) had shown a clear change over time, whereas a stable diversity was found with the mesophilic DOULEB well (DL3). PCR amplification of rRNA genes was unsuccessful with samples from DOULEB (DL12) at all three sampling time. The bacterial diversity present in production waters collected from pipelines of SERCINA and LITAYEM oilfields was high, while production waters collected from wellheads (ASHTART and DOULEB) exhibited lower diversity. The partial study of the biodiversity showed a dominance of uncultured bacteria and Pseudomonas genus (class of the Gammaproteobacteria) in three of the studied oilfields (ASHTART47, ASHTART48 and DOULEB3). However, for LITAYEM oilfield, a significant dominance of 5 phyla (Proteobacteria, Thermotogae, Firmicutes, Synergistetes, Bacteroidetes) was shown. Our study gives a real picture of the microbiology of some Tunisian oilfield production waters and shows that some of the sequenced bacterial clones have a great similarity to previous sequenced clones described from other oilfields all over the world, indicating that these ecosystems harbour specific microbial communities. These findings can be considered as an indirect indication of the indigenous origin of these clones.
An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant‐ants
Authors: Ruiz‐González Mario X. , Lauth Jérémie , Leroy Céline , Jauneau Alain , Gryta Hervé , Jargeat Patricia , Dejean Alain , Orivel Jérôme 
Published: May 2012

AbstractBecause of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant‐ants can be difficult to isolate with standard procedures. Here, we describe an easy‐to‐use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.
Impact of esophageal invasion on clinicopathological characteristics and long‐term outcome of adenocarcinoma of the subcardia
Authors: Tokunaga Masanori , Tanizawa Yutaka , Bando Etsuro , Kawamura Taiichi , Tsubosa Yasuhiro , Terashima Masanori 
Published: May 2012

AbstractBackgrounds and ObjectivesA different classification system was used in the 7th edition of the TNM classification for adenocarcinoma of the subcardia either with or without esophageal invasion. The aim of this study was to clarify the clinicopathological and survival impact of esophageal invasion.MethodsThe present study included 351 patients who underwent gastrectomy for adenocarcinoma located within 5 cm of the esophagogastric junction. The clinicopathological characteristics and survival curves were compared between patients with esophageal invasion [E (+) group, n = 125] and without esophageal invasion [E (−) group, n = 226].ResultsPatients in the E (+) group had more advanced disease. The 5‐year survival rate following macroscopic curative resection was significantly better in the E (−) group (80.8%) than in the E (+) (48.7%, P < 0.001), even after stratification by the pathological stage and nodal status. Multivariate analysis identified esophageal invasion (hazard ratio; 3.323, 95% confidential interval; 1.815–6.082) as one of the independent prognostic factors.ConclusionsEsophageal invasion affected the clinicopathological characteristics and long‐term outcome of patients. Further study is necessary to clarify whether patients with esophageal invasion should be classified using the system for esophageal cancer or by another method. J. Surg. Oncol. 2012; 106:856–861. © 2012 Wiley Periodicals, Inc.
The correlations between the expression of FGFR4 protein and clinicopathological parameters as well as prognosis of gastric cancer patients
Authors: Ye Yan‐Wei , Zhang Xiefu , Zhou Ye , Wu Jianghong , Zhao Chunlin , Yuan Lin , Wang Guojun , Du Chunyan , Wang Chunmeng , Shi Yingqiang 
Published: May 2012

AbstractBackgroundFibroblast growth factor receptor 4 (FGFR4) was seldom investigated in gastric cancer (GC). The purpose of the study was to elucidate the expression of FGFR4 protein in GC and related clinical significance.MethodsNinety‐four paraffin‐embedded tumor specimens were obtained from Cancer Hospital, Fudan University. The expression of FGFR4 as well as p53, p21, EGFR, neu, c‐myc, and PCNA were detected by immunohistochemical method. Then, correlation analysis and survival analysis were performed.ResultsThe expression rate of FGFR4 protein in GC tissues and normal stomach tissues was 93.6% and 30.8%, respectively (P = 0.000). The expression of FGFR4 was positively correlated with the expression of p21, neu and PCNA (P‐value was 0.009, 0.012, and 0.018, respectively). Subgroup analysis showed that compared to low expression group, the prognosis of patients with III/IV stage and negative expression of p21 in high expression group of FGFR4 were worse (P = 0.048, 0.041, respectively). Multivariate analysis showed that TNM stage was the independent prognostic factor in high expression group (HR, 11.593; 95% CI, 3.532–18.058;P = 0.000).ConclusionsHigh expression of FGFR4 protein, accelerating the progression of advanced GC, might be associated with a poor prognosis in patients with advanced FC. J. Surg. Oncol. 2012; 106:872–879. © 2012 Wiley Periodicals, Inc.
In vivo O‐Space imaging with a dedicated 12 cm Z2 insert coil on a human 3T scanner using phase map calibration
Authors: Stockmann Jason P. , Galiana Gigi , Tam Leo , Juchem Christoph , Nixon Terence W. , Constable R. Todd 
Published: May 2012

AbstractRecently, spatial encoding with nonlinear magnetic fields has drawn attention for its potential to achieve faster gradient switching within safety limits, tailored resolution in regions of interest, and improved parallel imaging using encoding fields that complement the sensitivity profiles of radio frequency receive arrays. Proposed methods can broadly be divided into those that use phase encoding (Cartesian‐trajectory PatLoc and COGNAC) and those that acquire nonlinear projections (O‐Space, Null space imaging, radial PatLoc, and 4D‐RIO). Nonlinear projection data are most often reconstructed with iterative algorithms that backproject data using the full encoding matrix. Just like conventional radial sequences that use linear spatial encoding magnetic fields, nonlinear projection methods are more sensitive than phase encoding methods to imperfect calibration of the encoding fields. In this work, voxel‐wise phase evolution is mapped at each acquired point in an O‐Space trajectory using a variant of chemical shift imaging, capturing all spin dynamics caused by encoding fields, eddy currents, and pulse timing. Phase map calibration is then applied to data acquired from a high‐power, 12 cm, Z2 insert coil with an eight‐channel radio frequency transmit‐receive array on a 3T human scanner. We show the first experimental proof‐of‐concept O‐Space images on in vivo and phantom samples, paving the way for more in‐depth exploration of O‐Space and similar imaging methods. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.
Three New Natural Compounds from the Root Bark Essential Oil from Xylopia aethiopica
Authors: Yapi Thierry Acafou , Boti Jean Brice , Attioua Barthelemy Koffi , Ahibo Antoine Coffy , Bighelli Ange , Casanova Joseph , Tomi Félix 
Published: May 2012

ABSTRACTIntroductionIn the course of on‐going work on the characterisation of aromatic plants from the Ivory Coast we investigated the composition of the root oil from Xylopia aethiopica.ObjectivesThe aim of this work was to investigate the chemical composition of X. aethiopica root oil and elucidate the structure of two new compounds.MethodologyAnalysis of the essential oil was carried out using a combination of chromatographic (CC, GC with retention indices) and spectroscopic techniques (MS, 13C‐NMR, 2D‐NMR).ResultsTwenty seven components, accounting for 95.6% of the whole composition, were identified including various compounds for which spectroscopic data were absent on commercial computerised MS libraries. Three compounds are reported for the first time as natural compounds and the structure of two new compounds, 4,4‐dimethyl‐2‐vinylcyclohexene and endo‐5‐methoxy‐3‐patchoulene, has been elucidated using extensive two‐dimensional NMR spectroscopy.ConclusionThe composition of X. aethiopica root oil is dominated by two dimethylvinylcyclohexene isomers. It differs drastically from the composition of leaf and fruit oils of the same plant. The combination of analytical techniques appeared crucial for a fruitful analysis. Copyright © 2012 John Wiley & Sons, Ltd.
One‐step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High‐speed Counter‐current Chromatography
Authors: Wang Xiaoyan , Wang Honglun , Ding Chenxu , Suo Yourui 
Published: May 2012

ABSTRACTIntroductionThe official rhubarb species have frequently been investigated for their hydroxyanthraquinone components and associated pharmacological properties. However, other unofficial rhubarb species were rarely studied until polyhydroxystilbenes (PHS), which commonly occur in the unofficial rhubarb, revealed a range of potential bioactivities. Hence, there has been increasing interest in the efficient preparation of high‐purity PHS for pharmacological and clinical trials.ObjectiveTo develop a suitable method for large‐scale preparative separation of PHS from the rhizome of Rheum likiangense Sam. by high‐speed counter‐current chromatography (HSCCC).ResultsTwo PHS compounds were isolated successfully within 440 min using a solvent system consisting of methanol:n‐butanol:chloroform:water (2:0.5:3:3, v/v/v/v). Eighty‐four milligrams of desoxyrhaponticin with 98.2% purity and 148 mg of rhaponticin with 95.3% purity were respectively yielded from 1.5 g of crude extract in the single, one‐step operation.ConclusionAn optimised HSCCC method has been established for large‐scale preparative separation of PHS compounds from Rheum likiangense Sam. Copyright © 2012 John Wiley & Sons, Ltd.
DNA sequencing of maternal plasma to identify Down syndrome and other trisomies in multiple gestations
Authors: Canick Jacob A. , Kloza Edward M. , Lambert‐Messerlian Geralyn M. , Haddow James E. , Ehrich Mathias , van den Boom Dirk , Bombard Allan T. , Deciu Cosmin , Palomaki Glenn E. 
Published: May 2012

ABSTRACTObjectiveStudies on prenatal testing for Down syndrome (trisomy 21), trisomy 18, and trisomy 13 by massively parallel shotgun sequencing (MPSS) of circulating cell free DNA have been, for the most part, limited to singleton pregnancies. If MPSS testing is offered clinically, it is important to know if these trisomies will also be identified in multiple pregnancies.MethodAmong a cohort of 4664 high‐risk pregnancies, maternal plasma samples were tested from 25 twin pregnancies (17 euploid, five discordant and two concordant for Down syndrome; one discordant for trisomy 13) and two euploid triplet pregnancies [Correction made here after initial online publication.]. Results were corrected for GC content bias. For each target chromosome (21, 18, and 13), z‐scores of 3 or higher were considered consistent with trisomy.ResultsSeven twin pregnancies with Down syndrome, one with trisomy 13, and all 17 twin euploid pregnancies were correctly classified [detection rate 100%, 95% confidence interval (CI) 59%–100%, false positive rate 0%, 95% CI 0%–19.5%], as were the two triplet euploid pregnancies.ConclusionAlthough study size is limited, the underlying biology combined with the present data provide evidence that MPSS testing can be reliably used as a secondary screening test for Down syndrome in women with high‐risk twin gestations. © 2012 John Wiley & Sons, Ltd.
Is fetal magnetic resonance imaging indicated when ultrasound isolated mild ventriculomegaly is present in pregnancies with no risk factors?
Authors: Parazzini Cecilia , Righini Andrea , Doneda Chiara , Arrigoni Filippo , Rustico Mariangela , Lanna Mariano , Triulzi Fabio 
Published: May 2012

ABSTRACTObjectiveVentriculomegaly (VM) is the most common brain anomaly in prenatal ultrasound (US) diagnosis. There is a general trend to perform fetal magnetic resonance imaging (MRI) when VM is severe (greater than 15 mm) and/or it is not isolated. The role of MRI is debated when VM is borderline (between 10 and 15 mm) and isolated. Some authors have subdivided borderline VM into mild (10 to 12 mm) and moderate (>12 to15 mm). The aim of the study was to evaluate the role of MR in the imaging protocol of fetal cases characterized by mild isolated VM and no risk factors.MethodAs a retrospective study, 179 fetal MRI exams (mean gestational age: 26 weeks), performed for mild, isolated VM on US, were analyzed to search additional or different findings with respect to ultrasound. The potential impact of MRI results on prenatal counselling is described.ResultsIn 49/179 cases, MRI and US results differed, but only in two of these cases did MRI studies provide clinically consistent additional information. In 130/179 cases, MRI confirmed US findings.ConclusionIn this extremely selected group of fetuses with isolated, mild VM and no risk factors, MRI may not be indicated in the prenatal imaging protocol. © 2012 John Wiley & Sons, Ltd.
Trends in antihypertensives use among Moroccan patients
Authors: Berrada El Azizi G. , Ahid S. , Ghanname I. , Belaiche A. , Hassar M. , Cherrah Y. 
Published: May 2012

ABSTRACTPurposeIn this study, we analyzed the consumption trends of antihypertensives in Morocco during the 1991–2010 period and the impacts after the institution of Mandatory Health Insurance and the marketing of generic drugs.MethodsWe used sales data from the Moroccan subsidiary of IMS Health “Intercontinental Marketing Service”. The consumption volumes were converted into defined daily doses (DDDs).ResultsBetween 1991 and 2010, outpatient consumption of antihypertensives went from 4.37 to 23.14 DDD/1000 inhabitants/day, a 5.30‐fold increase. In 2010, calcium channel blockers (CCBs) and angiotensin converting enzyme inhibitors (ACEI) were the most consumed (4.97 DDD/1000 inhabitants/day) for each one, followed by diuretics (4.20 DDD/1000 inhabitants/day). The most consumed products were amlodipine (4.27 DDD/1000 inhabitants/day) followed by ramipril (3.18 DDD/1000 inhabitants /day) and indapamide (1.72 DDD/1000inhabitants/day). Between 1991 and 2010, the consumption of generic antihypertensives went from 2% to 46%.ConclusionAntihypertensive consumption increased between 1991 and 2010. However, despite the increase of generic drugs consumption, the levels of antihypertensive consumption remain lower than the needs of hypertensive patients. Copyright © 2012 John Wiley & Sons, Ltd.
New, organo‐soluble, thermally stable aromatic polyimides and poly(amide‐imide) based on 2‐[5‐(3,5‐dinitrophenyl)‐1,3, 4‐oxadiazole‐2‐yl]pyridine
Authors: Mansoori Yagoub , Atghia Seyed Vahid , Sanaei Somyeh Shah , Zamanloo Mohammad Reza , Imanzadeh Gholamhassan , Eskandari Habibollah 
Published: May 2012

AbstractNew, thermally stable polyimides and a poly(amide‐imide) containing a 1,3,4‐oxadiazole‐2‐pyridyl pendant group based on 2‐[5‐(3,5‐diaminophenyl)‐1,3,4‐oxadiazole‐2‐yl]pyridine were synthesized. The synthesis and characterization of the model compound 2‐{5‐[(3,5‐bistrimellitimido)phenyl]‐1,3,4‐oxadiazole‐2‐yl}pyridine (DIDA) were also investigated, and DIDA was used in the preparation of the poly(amide‐imide) in an ionic liquid, 1‐butyl‐3‐methylimidazolium bromide, as a polymerization solvent. The polymers were characterized by separating and characterizing the poly(amic acid) intermediates using infrared and elemental analyses. The prepared polymers were soluble in polar and aprotic solvents, such as dimethylformamide, dimethylsulfoxide, N‐methyl‐2‐pyrrolidone and dimethylacetamide. Thermal behaviour of the polymers was studied using thermogravimetric analysis and differential scanning calorimetry. The inherent viscosities of the polyimide and poly(amide‐imide) solutions were in the range 0.34–0.85 dL g−1 (in concentrated sulfuric acid with a concentration of 0.125 g dL−1 at 25 ± 0.5 °C). The removal of Co(II) from aqueous solutions was performed using one of the polyimides. It was found that this polymer had a maximum adsorption capacity and efficiency at pH = 10.0. Copyright © 2012 Society of Chemical Industry