bluuebunny/crossref_metadata_2025_split huggingface dataset:
Musicoterapia durante o parto: Auxiliando no alívio da dor, uma revisão sistemática
Authors: Silva Loranny Santana , Vasconcelos Tawany Tavares Santos , Oliveira Halley Ferraro 
Published: May 2024

Objetivo: Apresentar os fundamentos e evidências que respaldam a aplicação da musicoterapia como uma ferramenta terapêutica eficaz no auxílio à dor do parto. Metodologia: O artigo trata-se de uma revisão bibliográfica, do tipo revisão sistemática, a partir de publicações científicas indexadas nas bases de dados Medline, PUBMED, Scopus, Lilacs, BDENF e Web of Science. Seguindo os critérios de inclusão e exclusão, selecionaram-se 12 artigos. Resultados: Através da análise dos dados encontrados, foi possível perceber que a terapia musical tem influência positiva, podendo diminuir a algia durante o parto. Ademais, diminui o nível de ansiedade e o tempo de TP, pois promove bem estar emocional, conforto e relaxamento na parturiente. Conclusões: Com base nas evidências disponíveis, a musicoterapia representa uma opção valiosa e acessível para mulheres que buscam um parto mais tranquilo e menos doloroso, destacando seu potencial para complementar e até mesmo substituir métodos tradicionais de alívio da dor durante o parto.
Konsumsi Buah Pisang Ambon (Musa Paradisiaca L Var Saplentum) dan Tablet Fe Terhadap Peningkatan Kadar Hemoglobin Pada Remaja Putri
Authors: BD Faridah , Fitriah Iin Prima , Saputri Lita Angelina , Bebasari Mardiani , Eravianti Eravianti , Merry Yussie Ater , Hidayah Fauziah Hasanatul 
Published: May 2024

Provinsi Sumatera Barat termasuk provinsi yang tidak mencapai target dalam pemberian tablet tambah darah (Fe) pada remaja puteri. Tablet Fe berguna untuk meningkatkan kadar Hb. Salah satu cara untuk meningkatkan kerja Fe dengan mengkonsumsi buah pisang ambon yang mengandung vitamin C. Berdasarkan data anemia Provinsi Sumatera Barat, angka kejadian anemia pada remaja putri sebesar 16,6%, sedangkan angka kejadian anemia di Kabupaten Pesisir Selatan pada tahun 2019 adalah sebesar 35,5%. Tujuan penelitian ini untuk mengetahui pengaruh pemberian tablet Fe dengan konsumsi Fe dan buah pisang ambon dalam meningkatkan kadar Hb.Jenis penelitian quasi eksperimen dengan rancangan two group pretest dan posttest design. Penelitian dilakukan tanggal 1 Juli s/d 8 Juli 2021 di SMA N 3 Painan. Populasi 68 orang siswa SMA N 3 Painan, penilitian ini menggunakan rumus Federer dengan jumlah sampel 32 orang. Teknik pengambilan sampel purposive sampling. Instrumen yang digunakan Hb digital dan lembar observasi. Pengolahan data uji pariet sampels T test.Rata-rata kadar Hb sebelum diberikan tablet Fe 11,00 gr/dl, rata-rata kadar Hb sebelum diberikan tablet Fe dan buah pisang ambon 11,00 gr/dl, dan rata-rata kadar hb setelah diberikan tablet Fe 12,29 gr/dl, dan rata-rata kadar Hb setelah diberikan tablet Fe dan buah pisang ambon 13,88 gr/dl. Pengaruh pemberian tablet Fe terhadap kenaikan kadar Hb dengan p value = 0,000. Pengaruh pemberian tablet Fe dan buah pisang ambon dengan p value = 0.000. Perbedaan efektifitas pemberian tablet Fe dengan pemberian tablet Fe dan buah pisang dengan p value = 0.000.Disimpulkan bahwa pemberian tablet Fe dan buah pisang ambon lebih efektif dibandingkan pemberian tablet Fe saja. Diharapkan dalam memberikan tablet Fe agar beriringan dengan pemberian buah pisang ambon bagi remaja putri yang mengalami anemia.
Efek Variasi Konsentrasi H3PO4 Terhadap Kualitas Karbon Aktif Cangkang Kemiri Berdasarkan Analisis Proksimat
Authors: Fiqriawan M. Ridho , Anas Muhammad , Erniwati  
Published: May 2024

Studi penggunaan H3PO4 sebagai aktivator kimiawi pada arang aktif cangkang kemiri dilakukan dengan tujuan untuk meningkatkan kualitas karbon aktif dari cangkang kemiri. Cangkang kemiri yang digunakan diperoleh dari Desa Ambaipua Kecamatan Ranomeeto Kabupaten Konawe Selatan Provinsi Sulawesi Tenggara. Penelitian dimulai dari proses pembuatan karbon aktif cangkang kemiri menjadi karbon aktif dengan suhu karbonasi 4000 C selama 8 jam. Kemudian untuk meningkatkan kualitas dari arang yang terbentuk, dilakukan aktivasi kimiawi dengan menggunakan larutan H3PO4 dengan konsentrasi 3M, 5M, 7M dan 9M dan dilanjutkan dengan aktivasi thermal pada suhu 7000 C selama 1 jam. Berdasarkan hasil pengujian melalui analisis proximat diperoleh bahwa karbon aktif yang diaktivasi dengan menggunakan aktivator H3PO4 dengan variasi konsentrasi 7M mempunyai hasil yang paling baik dengan kandungan karbon fix sebesar 88,5878%. Dimana hasil tersebut telah memenuhi standar SNI 06-3730-1995 yaitu minimal 65%.
Sintesis Silika Metode Sol-Gel Pada Material Tio2-Sio2 Terhadap Penurunan Kadar Kromium Dan Besi Dan Absorpsi Kelembaban Udara
Authors: Bimasakti  , Eddy Diana Rakhmawaty , Supardi Imam , Usman Ida , Aba La 
Published: May 2024

Silika merupakan material yang menarik perhatian para peneliti karena aplikasinya yang luas dan pembuatannya yang mudah dan mensintesis silika gel sebagai bahan untuk menyerap kelembaban udara. Bahan yang digunakan adalah TEOS (Tetraethylorthosilicate) sebagai precursor. Salah satu metode pembuatan silika adalah metode sol-gel. Silika yang dihasilkan bersifat amorf dengan densitas yang rendah sehingga dapat digunakan sebagai penyangga fotokatalis. Penelitian ini bertujuan untuk membuat fotokatalis TiO2-SiO2 untuk menurunkan kadar logam kromium dan besi dalam air. Tahapan penelitian adalah sintesis silica dengan variasi konsentrasi amonia dan suhu kalsinasi, imobilisasi TiO2 Pada SiO2 dan uji fotokatalisis TiO2-SiO2 terhadap kadar logam kromium dan besi dari air Sungai Cikijing. Untuk mengetahui gugus fungsi, morfologi, bentuk kristal, komposisi TiO2 dalam fotokatalis TiO2-SiO, dan kadar logam dilakukan analisis dengan menggunakan Fourier Transform-Infra Red (FT-IR), Scanning Electron Microscope (SEM-EDX), X-Ray Diffractometry (XRD) dan Atomic Absorbtion Spectroscopy (AAS). Dalam penelitian ini, jumlah konsentrasi amonia dan suhu kalsinasi divariasikan yaitu; 12,5 % dan 25% dengan suhu kalsinasi 4000 C, 5000 C, dan 6000 C. HCl sebagai katalis dan aquades sebagai pelarut, dengan metode Sol-Gel. Dalam penelitian ini waktu pemanasan sebagai variabel manipulasi, sehingga didapatkan sampel awal silika dalam bentuk serbuk yang berwarna putih. Untuk menjadikan silika serbuk ke dalam bentuk butiran, maka ditambahkan Natrium Silikat dengan perbandingan 1:2. Sehingga didapatkan silika gel dalam bentuk butiran dan memiliki warna putih. Silika gel yang sudah dihasilkan, kemudian dilakukan uji penyerap kelembaban dengan cara ditetesi air pada sekumpulan silika gel, sehingga dapat diketahui perubahan fisis yag terjadi pada silika gel. Dari hasil uji penyerap kelembaban dapat diindikasikan bahwa silika gel yang dihasilkan memiliki sifat hidrofilik (menyerap air), keras namun rapuh dan mengalami perubahan warna yang semula putih bening kemudian menjadi putih keruh, hal tersebut menujukkan bahwa silika gel dapat menyerap air. Uji karakterisasi SEM-EDS didapatkan bentuk morfologi yang tidak homogen dengan ukuran partikel pada sampel silika gel yang tidak seragam, karena dipengaruhi oleh lama waktu ageing, dan dari hasil analisis EDS didapatkan kandungan silika yang berbeda, karena dipengaruhi oleh lama waktu pemanasan yang berbeda pula. Dan hasil analisis XRD menunjukkan bahwa struktur kristal dari partikel silika gel SiO2 adalah amorf, dengan didapatkan pola difraksi puncak pada sudut 2θ = 22,53°.
Systematic metabolic engineering of Zymomonas mobilis for β-farnesene production
Authors: Xiao Yubei , Tan Xuemei , He Qiaoning , Yang Shihui 
Published: May 2024

Zymomonas mobilis is an ethanologenic bacterium that can produce hopanoids using farnesyl pyrophosphate (FPP), which can be used as the precursor by β-farnesene synthase for β-farnesene production. To explore the possibility and bottlenecks of developing Z. mobilis for β-farnesene production, five heterologous β-farnesene synthases were selected and screened, and AaBFS from Artemisia annua had the highest β-farnesene titer. Recombinant strains with AaBFS driven by the strong constitutive promoter Pgap (Pgap–AaBFS) doubled its β-farnesene production to 25.73 ± 0.31 mg/L compared to the recombinant strain with AaBFS driven by Ptet (Ptet–AaBFS), which can be further improved by overexpressing the Pgap–AaBFS construct using the strategies of multiple plasmids (41.00 ± 0.40 mg/L) or genomic multi-locus integration (48.33 ± 3.40 mg/L). The effect of cofactor NADPH balancing on β-farnesene production was also investigated, which can be improved only in zwf-overexpressing strains but not in ppnK-overexpressing strains, indicating that cofactor balancing is important and sophisticated. Furthermore, the β-farnesene titer was improved to 73.30 ± 0.71 mg/L by overexpressing dxs, ispG, and ispH. Finally, the β-farnesene production was increased to 159.70 ± 7.21 mg/L by fermentation optimization, including the C/N ratio, flask working volume, and medium/dodecane ratio, which was nearly 13-fold improved from the parental strain. This work thus not only generated a recombinant β-farnesene production Z. mobilis strain but also unraveled the bottlenecks to engineer Z. mobilis for farnesene production, which will help guide the future rational design and construction of cell factories for terpenoid production in non-model industrial microorganisms.
Characterizing carbapenemase-producing Escherichia coli isolates from Spain: high genetic heterogeneity and wide geographical spread
Authors: Dahdouh Elias , Gómez-Marcos Laro , Cañada-García Javier E. , de Arellano Eva Ramírez , Sánchez-García Aida , Sánchez-Romero Isabel , López-Urrutia Luis , de la Iglesia Pedro , Gonzalez-Praetorius Alejandro , Sotelo Jared , Valle-Millares Daniel , Alonso-González Isabela , Bautista Verónica , Lara Noelia , García-Cobos Silvia , Cercenado Emilia , Aracil Belén , Oteo-Iglesias Jesús , Pérez-Vázquez María ,   Spanish Eco-Carba Study Group
Published: May 2024

IntroductionCarbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically and genotypically characterizing CP-Eco isolates collected from Spain to better understand their resistance mechanisms and population structure.MethodsNinety representative isolates received from 2015 to 2020 from 25 provinces and 59 hospitals Spanish hospitals were included. Antibiotic susceptibility was determined according to EUCAST guidelines and whole-genome sequencing was performed. Antibiotic resistance and virulence-associated genes, phylogeny and population structure, and carbapenemase genes-carrying plasmids were analyzed.Results and discussionThe 90 CP-Eco isolates were highly polyclonal, where the most prevalent was ST131, detected in 14 (15.6%) of the isolates. The carbapenemase genes detected were blaOXA-48 (45.6%), blaVIM-1 (23.3%), blaNDM-1 (7.8%), blaKPC-3 (6.7%), and blaNDM-5 (6.7%). Forty (44.4%) were resistant to 6 or more antibiotic groups and the most active antibiotics were colistin (98.9%), plazomicin (92.2%) and cefiderocol (92.2%). Four of the seven cefiderocol-resistant isolates belonged to ST167 and six harbored blaNDM. Five of the plazomicin-resistant isolates harbored rmt. IncL plasmids were the most frequent (45.7%) and eight of these harbored blaVIM-1. blaOXA-48 was found in IncF plasmids in eight isolates. Metallo-β-lactamases were more frequent in isolates with resistance to six or more antibiotic groups, with their genes often present on the same plasmid/integron. ST131 isolates were associated with sat and pap virulence genes. This study highlights the genetic versatility of CP-Eco and its potential to disseminate ARGs and cause community and nosocomial infections.
Knowledge and attitude toward postoperative antithrombotic management and prevention in patients with coronary revascularization: a cross-sectional study
Authors: Liu Chunlu , Zhang Haijun , Yang Liming , Chen Lihua , Zu Changhao , Wang Fangfang , Dai Yunjia , Zhao Haiyan 
Published: May 2024

BackgroundThis study aimed to explore the knowledge and attitude (KA) toward postoperative antithrombotic management and prevention among coronary artery disease (CAD) patients who underwent coronary revascularization.MethodsThis cross-sectional study enrolled CAD outpatients and inpatients between May and December 2023 at Kailuan Medical Group at Tangshan. Basic demographic characteristics and KA scores were collected through a self-made questionnaire.ResultsThis study included 523 valid questionnaires. The mean knowledge and attitude scores were 13.20 ± 6.20 (range: 0–26) and 43.68 ± 6.01 (range: 21–50), respectively, indicating poor knowledge and favorable attitude. Multivariable logistic regression analysis showed that junior high school education (OR = 2.160, P = 0.035), high school or technical school education (OR = 2.356, P = 0.039), and monthly average income &gt;5,000 RMB (OR = 3.407, P = 0.002) were independently associated with knowledge. Knowledge (OR = 1.095, P = 0.002), BMI ≥ 24.0 kg/m2 (OR = 0.372, P = 0.011), junior high school (OR = 3.699, P = 0.002), high school or technical school (OR = 2.903, P = 0.028), high associate degree or above education (OR = 6.068, P = 0.014), monthly average income 3,000–5,000 RMB (OR = 0.296, P = 0.005), monthly average income &gt; 5,000 RMB (OR = 0.225, P = 0.021), with hypertension (OR = 0.333, P = 0.003), blood tests every 2–3 weeks (OR = 10.811, P = 0.011), blood tests every month (OR = 4.221, P = 0.024), and blood tests every 2–3 months (OR = 3.342, P = 0.033) were independently associated with attitude.ConclusionCAD patients who underwent coronary revascularization had poor knowledge but favorable attitudes toward postoperative antithrombotic management and prevention. The study underscores the need for targeted education, especially for individuals with lower education and income levels, ultimately improving patient compliance and cardiovascular outcomes.
Quantifiable impact: monitoring landscape restoration from space. A regreening case study in Tanzania
Authors: van der Vliet Mendy , Malbeteau Yoann , Ghent Darren , Haas Sander de , Veal Karen L. , van der Zaan Thijs , Sinha Rajiv , Dash Saroj K. , Houborg Rasmus , de Jeu Richard A. M. 
Published: May 2024

The impact of ecosystem conservation and restoration activities are rarely monitored from a global, multidimensional and multivariable perspective. Here we present an approach to quantify the environmental impact of landscape restoration using long-term and high-resolution satellite observations. For two restoration areas in Tanzania, we can likely attribute an increase in the amount of water retained by the soil (∼0.01 m³ m⁻³, ∼13% average increase), a soil temperature drop (∼-0.5°C) and an increase in surface greenness (∼50% average increase) in 3.5 years. These datasets illuminate the impact of restoration initiatives on the landscape and support the reporting of comprehensive metrics to donors and partners. Satellite observations from commercial providers and space agencies are now achieving the frequency, resolution, and accuracy that can allow for the effective evaluation of restoration activities.
IFNγ at the early stage induced after cryo-thermal therapy maintains CD4+ Th1-prone differentiation, leading to long-term antitumor immunity
Authors: Wang Junjun , Lou Yue , Wang Shicheng , Zhang Zelu , You Jiaqi , Zhu Yongxin , Yao Yichen , Hao Yuankai , Liu Ping , Xu Lisa X. 
Published: May 2024

IntroductionRecently, more and more research illustrated the importance of inducing CD4+ T helper type (Th)-1 dominant immunity for the success of tumor immunotherapy. Our prior studies revealed the crucial role of CD4+ Th1 cells in orchestrating systemic and durable antitumor immunity, which contributes to the satisfactory outcomes of the novel cryo-thermal therapy in the B16F10 tumor model. However, the mechanism for maintaining the cryo-thermal therapy-mediated durable CD4+ Th1-dominant response remains uncovered. Additionally, cryo-thermal-induced early-stage CD4+ Th1-dominant T cell response showed a correlation with the favorable prognosis in patients with colorectal cancer liver metastasis (CRCLM). We hypothesized that CD4+ Th1-dominant differentiation induced during the early stage post cryo-thermal therapy would affect the balance of CD4+ subsets at the late phase.MethodsTo understand the role of interferon (IFN)-γ, the major effector of Th1 subsets, in maintaining long-term CD4+ Th1-prone polarization, B16F10 melanoma model was established in this study and a monoclonal antibody was used at the early stage post cryo-thermal therapy for interferon (IFN)-γ signaling blockade, and the influence on the phenotypic and functional change of immune cells was evaluated.ResultsIFNγ at the early stage after cryo-thermal therapy maintained long-lasting CD4+ Th1-prone immunity by directly controlling Th17, Tfh, and Tregs polarization, leading to the hyperactivation of Myeloid-derived suppressor cells (MDSCs) represented by abundant interleukin (IL)-1β generation, and thereby further amplifying Th1 response.DiscussionOur finding emphasized the key role of early-phase IFNγ abundance post cryo-thermal therapy, which could be a biomarker for better prognosis after cryo-thermal therapy.
PD-1 inhibitor combined with paclitaxel and cisplatin in the treatment of recurrent and metastatic hypopharyngeal/laryngeal squamous cell carcinoma: efficacy and survival outcomes
Authors: Fang Qi , Li Xiaodi , Xu Pengfei , Cao Fei , Wu Di , Zhang Xinrui , Chen Chunyan , Gao Jianming , Su Yong , Liu Xuekui 
Published: May 2024

ObjectiveThis retrospective study analyzed the efficacy of PD-1 inhibitors combined with albumin-bound paclitaxel and cisplatin (TP regimen) in the treatment of recurrent and metastatic hypopharyngeal/laryngeal squamous cell carcinoma (RMHSCC/RMLSCC).MethodsPatients diagnosed and treated at the Sun Yat-sen University Cancer Center from August 1, 2020, to August 15, 2023, with histologically confirmed RMHSCC/RMLSCC were included. All patients received PD-1 inhibitors combined with albumin-bound paclitaxel (260mg/m2) and cisplatin (60mg/m2) for 3–4 cycles. The primary endpoints were overall survival (OS) and progression-free survival (PFS).ResultsA total of 50 patients with RMHSCC/RMLSCC who received TP+PD-1 inhibitor therapy were included, with an objective response rate (ORR) of 56.0% (28/50). The 1-year and 2-year OS rates were 80.2% (95% CI: 69.3%-92.9%) and 68.6% (95% CI: 52.6%-89.5%), respectively, while the 1-year and 2-year PFS rates were 44.7% (95% CI: 31.9%-62.5%) and 26.0% (95% CI: 12.6%-53.4%), respectively. Treatment-related adverse events mainly included rash, myelosuppression, gastrointestinal reactions, and hypothyroidism.ConclusionIn the treatment of RMHSCC/RMLSCC with TP + PD-1 inhibitors, survival rates of patients can be improved while ensuring the safety of the treatment regimen.
Mincle receptor in macrophage and neutrophil contributes to the unresolved inflammation during the transition from acute kidney injury to chronic kidney disease
Authors: Wang Cui , Zhang Yilin , Shen Anran , Tang Taotao , Li Ning , Xu Chuanhui , Liu Bicheng , Lv Linli 
Published: May 2024

BackgroundRecent studies have demonstrated a strong association between acute kidney injury (AKI) and chronic kidney disease (CKD), while the unresolved inflammation is believed to be a driving force for this chronic transition process. As a transmembrane pattern recognition receptor, Mincle (macrophage-inducible C-type lectin, Clec4e) was identified to participate in the early immune response after AKI. However, the impact of Mincle on the chronic transition of AKI remains largely unclear.MethodsWe performed single-cell RNA sequencing (scRNA-seq) with the unilateral ischemia-reperfusion (UIR) murine model of AKI at days 1, 3, 14 and 28 after injury. Potential effects and mechanism of Mincle on renal inflammation and fibrosis were further validated in vivo utilizing Mincle knockout mice.ResultsThe dynamic expression of Mincle in macrophages and neutrophils throughout the transition from AKI to CKD was observed. For both cell types, Mincle expression was significantly up-regulated on day 1 following AKI, with a second rise observed on day 14. Notably, we identified distinct subclusters of Minclehigh neutrophils and Minclehigh macrophages that exhibited time-dependent influx with dual peaks characterized with remarkable pro-inflammatory and pro-fibrotic functions. Moreover, we identified that Minclehigh neutrophils represented an “aged” mature neutrophil subset derived from the “fresh” mature neutrophil cluster in kidney. Additionally, we observed a synergistic mechanism whereby Mincle-expressing macrophages and neutrophils sustained renal inflammation by tumor necrosis factor (TNF) production. Mincle-deficient mice exhibited reduced renal injury and fibrosis following AKI.ConclusionThe present findings have unveiled combined persistence of Minclehigh neutrophils and macrophages during AKI-to-CKD transition, contributing to unresolved inflammation followed by fibrosis via TNF-α as a central pro-inflammatory cytokine. Targeting Mincle may offer a novel therapeutic strategy for preventing the transition from AKI to CKD.
Exploring the gut microbiota-hippocampus-metabolites axis dysregulation in sepsis mice
Authors: Song Fangqiang , Li Qinglun , Cui Jiyao , Wang Jianhua , Xiao Shuai , Yu Bo , Sun Yanqi , Song Wenke , Wu Linlin , Zhou Yongqin 
Published: May 2024

BackgroundSepsis is commonly associated with a sudden impairment of brain function, thus leading to significant rates of illness and mortality. The objective of this research was to integrate microbiome and metabolome to reveal the mechanism of microbiota-hippocampus-metabolites axis dysfunction in a mouse model of sepsis.MethodsA mouse model of sepsis was established via cecal ligation and puncture. The potential associations between the composition of the gut microbiota and metabolites in the hippocampus of mice with sepsis were investigated by combining 16S ribosomal RNA gene sequencing and ultra-high-performance liquid chromatography tandem mass spectrometry.ResultsA total of 140 differential metabolites were identified in the hippocampal tissues of mice with sepsis when compared to those of control mice. These differential metabolites in mice with sepsis were not only associated with autophagy and serotonergic synapse, but also involved in the metabolism and synthesis of numerous amino acids. At the phylum level, the abundance of Bacteroidota was increased, while that of Firmicutes (Bacillota) was decreased in mice with sepsis. At the genus level, the abundance of Alistipes was increased, while that of Lachnospiraceae_NK4A136_group was decreased in mice with sepsis. The Firmicutes (Bacillota)/Bacteroidota (F/B) ratio was decreased in mice with sepsis when compared to that of control mice. Furthermore, the F/B ratio was positively correlated with 5′-methylthioadenosine, PC (18:3(9Z,12Z,15Z)/18:0) and curdione, and negatively correlated with indoxylsulfuric acid, corticosterone, kynurenine and ornithine.ConclusionAnalysis revealed a reduction in the F/B ratio in mice with sepsis, thus contributing to the disturbance of 5′-methylthioadenosine, curdione, PC (18:3(9Z,12Z,15Z)/18:0), corticosterone, ornithine, indoxylsulfuric acid and kynurenine; eventually, these changes led to hippocampus dysfunction. Our findings provide a new direction for the management of sepsis-induced hippocampus dysfunction.