bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Term Effects of Sildenafil in a Rat Model of Chronic Mitral Regurgitation
Authors: Kim Kyung-Hee , Kim Yong-Jin , Ohn Jung-Hun , Yang Jimin , Lee Sang-Eun , Lee Sae-Won , Kim Hyung-Kwan , Seo Jeong-Wook , Sohn Dae-Won 
Published: February 2012

Background—
We tested the hypothesis that chronic treatment with sildenafil attenuates left ventricular (LV) remodeling and prevents exercise intolerance in chronic mitral regurgitation (MR).

Methods and Results—

MR was created in Sprague-Dawley rats by making a hole on the mitral leaflet. Two weeks after MR creation, MR and LV dilatation were confirmed by echocardiography, and rats were randomly assigned to sildenafil treatment (MR+sildenafil group; 50 mg/kg PO twice a day; n=16) or normal saline only (MR group; n=16) and continued for 4 months. Sixteen sham rats were compared with MR rats. After 4 months, LV size was smaller in the MR+sildenafil compared with the MR group (LV end-systolic dimension, 4.7±0.3 for sham versus 5.9±0.3 for MR+sildenafil versus 7.4±0.5 mm for MR;
P
<0.05; LV end-diastolic dimension, 8.3±0.4 versus 10.5±0.2 versus 11.7±0.61 mm, respectively;
P
<0.05). LV ejection fraction was greater in the MR+sildenafil group than in the MR group (70.2±2.2 for sham versus 67.0±4.2 for MR+sildenafil versus 58.9±2.5 for MR;
P
=0.01). Serial treadmill test revealed that exercise capacity was reduced in the MR but not in the MR+sildenafil group. Transcriptional profiling of cardiac apical tissues revealed that gene sets related to inflammatory response, DNA damage response, cell cycle checkpoint, and cellular signaling pathways were significantly enriched by genes with reciprocal changes. Pathological analysis showed that perivascular fibrosis was more prominent in the MR than in the MR+sildenafil group and that the percentage of terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling–positive cells was 2-fold greater in the MR compared with the MR+sildenafil group.

Conclusions—
Sildenafil significantly attenuates LV remodeling and prevents exercise intolerance in a rat model of chronic MR. This benefit may be associated with the antiapoptotic, anti-inflammatory effects of sildenafil.
Seasonal Variability in the Diurnal Evolution of the Boundary Layer in a Near-Coastal Urban Environment
Authors: Haman Christine L. , Lefer Barry , Morris Gary A. 
Published: February 2012

Abstract
Boundary layer height is estimated during a 21-month period in Houston, Texas, using continuous ceilometer observations and the minimum-gradient method. A comparison with over 60 radiosondes indicates overall agreement between ceilometer- and radiosonde-estimated PBL and residual layer heights. Additionally, the ceilometer-estimated PBL heights agree well with 31 vertical profiles of ozone. Difficulty detecting the PBL height occurs immediately following a frontal system with precipitation, during periods with high wind speeds, and in the early evening when convection is weakening, a new stable surface layer is forming, and the lofted aerosols detected by the lidar do not represent the PBL. Long-term diurnal observations of the PBL height indicate nocturnal PBL heights range from approximately 100 to 300 m throughout the year, while the convective PBL displays more seasonal and daily variability typically ranging from 1100 m in the winter to 2000 m in the summer.
New Methods for Estimating Ocean Eddy Heat Transport Using Satellite Altimetry
Authors: Keating Shane R. , Majda Andrew J. , Smith K. Shafer 
Published: February 2012

Abstract
Attempts to monitor ocean eddy heat transport are strongly limited by the sparseness of available observations and the fact that heat transport is a quadratic, sign-indefinite quantity that is particularly sensitive to unresolved scales. In this article, a suite of stochastic filtering strategies for estimating eddy heat transport are tested in idealized two-layer simulations of mesoscale oceanic turbulence at high and low latitudes under a range of observation scenarios. A novel feature of these filtering strategies is the use of computationally inexpensive stochastic models to forecast the underlying nonlinear dynamics. The stochastic model parameters can be estimated by regression fitting to climatological energy spectra and correlation times or by adaptively learning these parameters “on-the-fly” from the observations themselves.
The authors show that, by extracting high-wavenumber information that has been aliased into the low wavenumber band, “stochastically super-resolved” velocity fields with a nominal resolution increase of a factor of 2 or more can be derived. Observations of the upper-layer streamfunction are projected onto an empirical orthogonal function basis for the vertical structure to produce filtered estimates for both upper- and lower-layer streamfunctions and hence net heat transport. The resulting time-mean poleward eddy heat transport is significantly closer to the true value when compared with standard estimates based upon optimal interpolation. By contrast, the temporal variability of the heat transport is underestimated because of poor temporal resolution. Implications for estimating poleward eddy heat transport using current and next-generation altimeters are discussed.
The antitumor effects of tetrodotoxin and/or doxorubicin on Ehrlich ascites carcinoma-bearing female mice
Authors: El-Dayem Samiha M Abd , Fouda Fatma M , Ali Elham H A , Motelp Bosy A Abd El 
Published: February 2012

The study aimed to investigate the antitumor effect of tetrodotoxin (TTX) and/or doxorubicin (DOX) on Ehrlich ascites carcinoma (EAC)-bearing mice through the investigated biochemical parameters. TTX and/or DOX with or without N-acetylcystiene were administrated after 10 days into EAC-female mice for a period of 2 weeks in six equal doses. Treatment with TTX or DOX caused a significant decrease in the mean tumor weight and an increase in the cumulative mean survival time when compared with EAC group. All the treatments reduced the elevated liver tumor markers and increased liver antioxidant enzymes under investigation in comparison with EAC. Hepatic cells, suffered severely from degeneration and karriolysis in EAC group, revealed some improvement as appearance of healthy hepatocytes by TTX treatment. The present results suggested that TTX had a more powerful inhibitor effect on EAC growth than DOX and TTX plus DOX treatments reflected by antitumor biochemical and histological studies.
OpenMP task scheduling strategies for multicore NUMA systems
Authors: Olivier Stephen L , Porterfield Allan K , Wheeler Kyle B , Spiegel Michael , Prins Jan F 
Published: February 2012

The recent addition of task parallelism to the OpenMP shared memory API allows programmers to express concurrency at a high level of abstraction and places the burden of scheduling parallel execution on the OpenMP run-time system. Efficient scheduling of tasks on modern multi-socket multicore shared memory systems requires careful consideration of an increasingly complex memory hierarchy, including shared caches and non-uniform memory access (NUMA) characteristics. In order to evaluate scheduling strategies, we extended the open source Qthreads threading library to implement different scheduler designs, accepting OpenMP programs through the ROSE compiler. Our comprehensive performance study of diverse OpenMP task-parallel benchmarks compares seven different task-parallel run-time scheduler implementations on an Intel Nehalem multi-socket multicore system: our proposed hierarchical work-stealing scheduler, a per-core work-stealing scheduler, a centralized scheduler, and LIFO and FIFO versions of the Qthreads round-robin scheduler. In addition, we compare our results against the Intel and GNU OpenMP implementations. Our hierarchical scheduling strategy leverages different scheduling methods at different levels of the hierarchy. By allowing one thread to steal work on behalf of all of the threads within a single chip that share a cache, the scheduler limits the number of costly remote steals. For cores on the same chip, a shared LIFO queue allows exploitation of cache locality between sibling tasks as well as between a parent task and its newly created child tasks. In the performance evaluation, our Qthreads hierarchical scheduler is competitive on all benchmarks tested. On five of the seven benchmarks, it demonstrates speedup and absolute performance superior to both the Intel and GNU OpenMP run-time systems. Our run-time also demonstrates similar performance benefits on AMD Magny Cours and SGI Altix systems, enabling several benchmarks to successfully scale to 192 CPUs of an SGI Altix.
Quality of life of caregivers of children with autism in Qatar
Authors: Kheir Nadir , Ghoneim Ola , Sandridge Amy L. , Al-Ismail Muna , Hayder Sara , Al-Rawi Fadhila 
Published: February 2012

Introduction: Caring for a child diagnosed with autism could affect the quality of life of the caregiver in various different ways. No previous research has assessed the quality of lives of caregivers of children with autism in Qatar. Methods: Caregivers of a child with autism between 3 and 17 years old were recruited from child rehabilitation clinics in Qatar. The non-autism group was represented by caregivers of a typically growing child visiting a primary health care facility for a routine medical examination. Data collected from both groups included demographic and quality of life information for caregivers. Results: A total of 98 participants consented to take part in the study. Fifty-six of these were caregivers of a child with autism and 42 were caregivers of a typically growing child. There was no significant difference between quality of life domains between the two groups of caregivers, but caregivers of autistic children rated their health as poor and likely to get worse (p < 0.05). Conclusions: This study provided some evidence for the impact of caring for a child with autism on the life of the caregiver. The findings should help health policy-makers in Qatar to provide better and more focused support to children with autism and their caregivers.
Knock-in of a FLT3/ITD mutation cooperates with a NUP98-HOXD13 fusion to generate acute myeloid leukemia in a mouse model
Authors: Greenblatt Sarah , Li Li , Slape Christopher , Nguyen Bao , Novak Rachel , Duffield Amy , Huso David , Desiderio Stephen , Borowitz Michael J. , Aplan Peter , Small Donald 
Published: February 2012

Abstract
Constitutive activation of FLT3 by internal tandem duplication (ITD) is one of the most common molecular alterations in acute myeloid leukemia (AML). FLT3/ITD mutations have also been observed in myelodysplastic syndrome patients both before and during progression to AML. Previous work has shown that insertion of an FLT3/ITD mutation into the murine Flt3 gene induces a myeloproliferative neoplasm, but not progression to acute leukemia, suggesting that additional cooperating events are required. We therefore combined the FLT3/ITD mutation with a model of myelodysplastic syndrome involving transgenic expression of the Nup98-HoxD13 (NHD13) fusion gene. Mice expressing both the FLT3/ITD and NHD13 transgene developed AML with 100% penetrance and short latency. These leukemias were driven by mutant FLT3 expression and were susceptible to treatment with FLT3 tyrosine kinase inhibitors. We also observed a spontaneous loss of the wild-type Flt3 allele in these AMLs, further modeling the loss of the heterozygosity phenomenon that is seen in human AML with FLT3-activating mutations. Because resistance to FLT3 inhibitors remains an important clinical issue, this model may help identify new molecular targets in collaborative signaling pathways.
Elevated serum IL-10 levels in diffuse large B-cell lymphoma: a mechanism of aberrant JAK2 activation
Authors: Gupta Mamta , Han Jing Jing , Stenson Mary , Maurer Matthew , Wellik Linda , Hu Guangzhen , Ziesmer Steve , Dogan Ahmet , Witzig Thomas E. 
Published: February 2012

AbstractCytokines are deregulated in cancers and can contribute to tumor growth. In patients with diffuse large-cell lymphoma (DLBCL), we observed higher levels of JAK/STAT pathway-related serum cytokines (ie, IL-6, IL-10, epidermal growth factor, and IL-2) compared with controls. Of these, only IL-10 activated the JAK2 pathway in lymphoma cells in vitro. Patients with high serum IL-10 had shorter event-free survival (EFS) than patients with low levels (P > .01) and high IL-10 was correlated with high lactase dehydrogenase (P = .0085) and higher International Prognostic Index scores (P = .01). To explore the mechanism by which IL-10 may contribute to an inferior EFS, we investigated the effect of IL-10 on the JAK2 pathway and found that the IL-10/IL-10 receptor complex up-regulated JAK2 signaling. Neutralizing Ab to IL-10 inhibited constitutive and IL-10–induced JAK2/STAT3 phosphorylation. JAK2 inhibition dephosphorylated JAK2 and STAT3 and caused an inhibitory effect on phospho-JAK2–positive DLBCL cells; there was a minimal effect on phospho-JAK2–negative cells. Apoptosis induced by JAK2 inhibition was dependent on inhibition of autocrine IL-10 and c-myc expression and independent of Bcl-2 family expression. These results provide the rationale for testing JAK2 inhibitors in DLBCL patients, and indicate that serum IL-10 may be a biomarker to identify patients more likely to respond to JAK2-targeted therapy.
Targeting Gene-Viro-Therapy with AFP driving Apoptin gene shows potent antitumor effect in hepatocarcinoma
Authors: Zhang Kang-Jian , Qian Jing , Wang Shi-Bing , Yang Yi 
Published: February 2012

AbstractBackgroundGene therapy and viral therapy are used for cancer therapy for many years, but the results are less than satisfactory. Our aim was to construct a new recombinant adenovirus which is more efficient to kill hepatocarcinoma cells but more safe to normal cells.MethodsBy using the Cancer Targeting Gene-Viro-Therapy strategy, Apoptin, a promising cancer therapeutic gene was inserted into the double-regulated oncolytic adenovirus AD55 in which E1A gene was driven by alpha fetoprotein promoter along with a 55 kDa deletion in E1B gene to form AD55-Apoptin. The anti-tumor effects and safety were examined by western blotting, virus yield assay, real time polymerase chain reaction, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Hoechst33342 staining, Fluorescence-activated cell sorting, xenograft tumor model, Immunohistochemical assay, liver function analysis and Terminal deoxynucleotidyl transferase mediated dUTP Nick End Labeling assay.ResultsThe recombinant virus AD55-Apoptin has more significant antitumor effect for hepatocelluar carcinoma cell lines (in vitro) than that of AD55 and even ONYX-015 but no or little impair on normal cell lines. Furthermore, it also shows an obvious in vivo antitumor effect on the Huh-7 liver carcinoma xenograft in nude mice with bigger beginning tumor volume till about 425 mm3 but has no any damage on the function of liver. The induction of apoptosis is involved in AD55-Apoptin induced antitumor effects.ConclusionThe AD55-Apoptin can be a potential anti-hepatoma agent with remarkable antitumor efficacy as well as higher safety in cancer targeting gene-viro-therapy system.
Web-browser encryption of personal health information
Authors: Morse Richard E , Nadkarni Prakash , Schoenfeld David A , Finkelstein Dianne M 
Published: February 2012

Abstract

Background
Electronic health records provide access to an unprecedented amount of clinical data for research that can accelerate the development of effective medical practices. However it is important to protect patient confidentiality, as many medical conditions are stigmatized and disclosure could result in personal and/or financial loss.

Results
We describe a system for remote data entry that allows the data that would identify the patient to be encrypted in the web browser of the person entering the data. These data cannot be decrypted on the server by the staff at the data center but can be decrypted by the person entering the data or their delegate. We developed this system to solve a problem that arose in the context of clinical research, but it is applicable in a range of situations where sensitive information is stored and updated in a database and it is necessary to ensure that it cannot be viewed by any except those intentionally given access.

Conclusion
By developing this system, we are able to centralize the collection of some patient data while minimizing the risk that protected health information be made available to study personnel who are not authorized to use it.
Malaria transmission in Libreville: results of a one year survey
Authors: Mourou Jean-Romain , Coffinet Thierry , Jarjaval Fanny , Cotteaux Christelle , Pradines Eve , Godefroy Lydie , Kombila Maryvonne , Pagès Frédéric 
Published: February 2012

Abstract

Background
In Gabon, vector transmission has been poorly studied. Since the implementation of the Roll Back malaria recommendations, clinical studies have shown a decline in the burden of malaria in Libreville, the capital city of Gabon. To better understand the transmission dynamic in Libreville, an entomological survey was conducted in five districts of the city.

Methods
Mosquitoes were sampled by human landing collection during 1 year in five districts of Libreville: Alibandeng, Beauséjour, Camp des Boys and Sotega. Mosquitoes were identified morphologically and by molecular methods. The Plasmodium falciparum circumsporozoïte indices were measured by ELISA, and the entomological inoculation rates (EIR) were calculated for all areas. Molecular assessments of pyrethroid knock down resistance (kdr) and of insensitive acetylcholinesterase resistance were conducted.

Results
A total of 57,531 mosquitoes were caught during 341 person-nights (161 person-nights indoor and 180 person-nights outdoor) among which, 4,223 were Anopheles gambiae s.l. The average Human Biting Rate fell from 15.5 bites per person during the rainy season to 4.7 during the dry season. The An. gambiae complex population was composed of An. gambiae s.s molecular form S (99.5%), Anopheles melas (0.3%) and An. gambiae s.s. form M (0.2%). Thirty-three out of 4,223 An. gambiae s.l. were found to be infected by P. falciparum (CSP index = 0.78%). The annual EIR was estimated at 33.9 infected bites per person per year ranging from 13 in Alibandeng to 88 in Sotega. No insensitive AChE mutation was identified but both kdr-w and kdr-e mutations were present in An. gambiae molecular form S with a higher frequency of the kdr-w allele (76%) than the kdr-e allele (23.5%).

Conclusion
Malaria transmission in Libreville occurred mainly during the rainy season but also during the dry season in the five districts. Transmission level is high and seems to be very heterogeneous in the town. Interestingly, the highest EIR was recorded in the most central and urbanized quarter and the lowest in a peripheral area. The decrease of transmission usually seen from peri-urban areas to urban centers is probably more dependent of the socio-economic level of a quarter than of its location in the city. Urban malaria control programmes need to consider the socio economic level of an area rather than the location in the city in order to determine the areas most favourable to malaria transmission.
First Initial community-acquired meningitis due to extended-spectrum beta-lactamase producing Escherichia coli complicated with multiple aortic mycotic aneurysms
Authors: Weyrich Pierre , Ettahar Nicolas , Legout Laurence , Meybeck Agnes , Leroy Olivier , Senneville Eric 
Published: February 2012

Abstract
We report the first case of extended-spectrum beta-lactamase producing E. coli community-acquired meningitis complicated with multiple aortic mycotic aneurysms. Because of the acute aneurysm expansion with possible impending rupture on 2 abdominal CT scan, the patient underwent prompt vascular surgery and broad spectrum antibiotic therapy but he died of a hemorrhagic shock. Extended-spectrum beta-lactamase producing E. coli was identified from both blood and cerebrospinal fluid culture before vascular treatment. The present case report does not however change the guidelines of Gram negative bacteria meningitis in adults.
Serial measurements of cardiac biomarkers in patients after allogeneic hematopoietic stem cell transplantation
Authors: Roziakova Lubica , Bojtarova Eva , Mistrik Martin , Dubrava Juraj , Gergel Jozef , Lenkova Nadezda , Mladosievicova Beata 
Published: February 2012

Abstract

Background
Previous therapy with anthracyclines (ANT) and conditioning regimen followed by hematopoietic stem cell transplantation (HSCT) represents a high risk for development of cardiotoxicity. The aim of this study was to assess subclinical myocardial damage after HSCT using echocardiography and cardiac biomarkers - high sensitive cardiac troponin T (hs-cTnT) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) and to identify patients at risk of developing clinical cardiotoxicity.

Patients and methods
Thirty-seven patients who were treated with allogeneic HSCT for hematologic diseases at median age of 28 years at time of HSCT were studied. Conditioning regimen included either chemotherapy without total body irradiation (TBI) or combination of chemotherapy with TBI. Twenty-nine (78,3%) patients were pretreated with ANT therapy. Cardiac biomarkers were serially measured before conditioning regimen and at days 1, 14 and 30 after HSCT. Cardiac systolic and diastolic functions were assessed before conditioning regimen and 1 month after HSCT by echocardiography.

Results
The changes in plasma NT-proBNP and hs-cTnT levels during the 30 days following the HSCT were statistically significant (P < 0,01 v.s. P < 0,01). Persistent elevations of NT-proBNP and hs-cTnT simultaneously for a period exceeding 14 days after HSCT were found in 29,7% patients. Serum concentrations of cardiomarkers were significantly elevated in ANT group compared to non-ANT group. These observations were underscored by the echocardiographic studies which did reveal significant changes in systolic and diastolic parameters. Five of 37 (13,5%) patients developed clinical manifestation of cardiotoxicity.

Conclusions
Elevations in both cardiac biomarkers were found before clinical signs of cardiotoxicity developed. Persistent elevations in NT-pro-BNP and hs-cTnT concentrations simultaneously for a period exceeding 14 days might be used for identification of patients at risk of developing cardiotoxicity and requiring further cardiological follow up.