bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of the growth factors PDGF‐BB, TGF‐β1 and bFGF on the replicative aging of human articular chondrocytes during in vitro expansion
Authors: Brandl Anita , Angele Peter , Roll Christina , Prantl Lucas , Kujat Richard , Kinner Bernd 
Published: October 2009

AbstractDecreasing replicative potential and dedifferentiation of articular chondrocytes during expansion in cell culture are essential limitations for tissue engineering and cell therapy approaches. Telomeres and telomerase play a key role in cell development, aging, and tumorigenesis. There is evidence that growth factors are involved in regulating telomerase activity. Therefore, the objective was to evaluate the effect of selected growth factors on telomere biology of serially passaged chondrocytes. Human articular chondrocytes were isolated from cartilage of three patients undergoing total knee arthroplasty. The chondrocytes were cultured in monolayer with the growth factors PDGF‐BB, TGF‐β1, and bFGF. Telomere length was measured by telomere restriction fragment length assay, and telomerase activity was determined by quantifying the gene expression of its catalytic subunit hTERT by rtPCR. Chondrocytes cultured with PDGF‐BB and TGF‐β1 showed a significantly higher proliferation rate than control cells. None of the growth factor cultures revealed an accelerated rate of telomere shortening. Telomerase was not expressed in significant amounts in any of the chondrocyte cultures. Growth factor treatment of chondrocyte cell cultures for cell therapy purposes can be regarded as safe in terms of telomere biology. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:354–360, 2010
Comparative study on antioxidant properties of carrot juice stabilised by high‐intensity pulsed electric fields or heat treatments
Authors: Quitão‐Teixeira Luciano José , Odriozola‐Serrano Isabel , Soliva‐Fortuny Robert , Mota‐Ramos Afonso , Martín‐Belloso Olga 
Published: October 2009

AbstractBACKGROUND:The effect of high‐intensity pulsed electric field (HIPEF) processing (35 kV cm−1for 1500 µs using 6‐µs bipolar pulses at 200 Hz) on the antioxidant features (vitamin C, β‐carotene, total phenolic compounds and antioxidant capacity) of carrot juice as well as on peroxidase activity was investigated and compared to the observed in heat pasteurised juices (90 °C for 60 s or 30 s) having the fresh juice as a reference.RESULTS:HIPEF and heat‐treated carrot juices had higher β‐carotene and lower vitamin C contents than the untreated juices immediately after processing. The antioxidant capacity of the juices was significantly modified neither by HIPEF nor by thermal treatments. POD activity decreased drastically (≥93.3%) after processing irrespective of the treatment applied. Vitamin C and β‐carotene content decreased throughout the storage following an exponential trend (R2= 0.801–0.984) with degradation rates between 1.7 × 10−2and 3.5 × 10−2day−1. Vitamin C and β‐carotene contents were better maintained in HIPEF‐treated than in heat‐pasteurised juices throughout the storage. Total phenolic content and the antioxidant capacity of the HIPEF‐treated juice did not substantially differ from that of the thermally treated juice for 56 days.CONCLUSION:HIPEF processing may help to achieve fresh‐like carrot juices with increased amounts of health‐related phytochemicals. Copyright © 2009 Society of Chemical Industry
Impact of postoperative irradiation after non‐curative resection of hilar biliary cancer
Authors: Kobayashi Shogo , Nagano Hiroaki , Marubashi Shigeru , Takeda Yutaka , Tanemura Masahiro , Konishi Koji , Yoshioka Yasuo , Inoue Takehiro , Doki Yuichiro , Mori Masaki 
Published: October 2009

AbstractBackgroundThe aim of this retrospective study was to determine the effect of surgical margin on the outcome of postoperative radiotherapy (RT) in patients with resected hilar biliary cancer.MethodsThe study subjects were 87 patients with hilar biliary cancer resected surgically before 2008. Based on the Japanese Society of Biliary Surgery (JSBS) criteria for diagnosis of biliary cancer, the surgical margin status was categorized as margin 1 (histopathologically margin negative, but cancer cells identified within 5 mm from the margin), margin 2 (histopathologically margin positive), and margin 0 (other margin status).ResultsThe surgical margin was 1 or 2 in 44 patients and 21 of these patients underwent RT. The 3‐year overall survival and progression‐free survival rates of the RT and non‐RT groups were 47% and 23% (P = 0.0392), and 49% and 19% (P = 0.0197), respectively. Univariate and multivariate analyses identified RT as the only factor that influenced survival. Subgroup analysis showed that the effect of RT was dependent on pathologically negative lymph node metastasis and positive margin (margin 2).ConclusionPostoperative RT is beneficial for patients with margins 1 and 2, especially those who are lymph node metastasis negative and have histopathologically positive margin. J. Surg. Oncol. 2009;100:657–662. © 2009 Wiley‐Liss, Inc.
Relationship of polymorphism of the tandem repeat sequence in the thymidylate synthase gene and the survival of stage III colorectal cancer patients receiving adjuvant 5‐flurouracil‐based chemotherapy
Authors: Park Chi‐Min , Lee Woo Yong , Chun Ho‐Kyung , Cho Yong Beom , Yun Hae Ran , Heo Jin Seok , Yun Seong Hyeon , Kim Hee Cheol 
Published: October 2009

AbstractBackgroundThe aim of this study was to determine whether the different polymorphisms in the thymidylate synthase (TS) gene, novel G>C single nucleotide polymorphism (SNP) and variable number of tandem repeat (VNTR), may be related with disease‐free survival (DFS) in patients with stage III colorectal cancer receiving adjuvant chemotherapy.MethodsThe study included 201 patients with pathologic TNM stage III colon cancer who received adjuvant 5‐fluorouracil (5‐FU)‐based chemotherapy after surgery. DNA was extracted from fresh tumor tissue and sequenced. Patients with TS genotypes of 2R3G, 3C3G, or 3G3G were assigned to a high expression group, and those with 2R2R, 2R3C, or 3C3C, to a low expression group.ResultsFrequencies of the TS tandem repeat polymorphisms among the tumor genotypes were 6.0% in 2R2R, 25.4% in 2R3R, and 68.7% in 3R3R. The low expression group included 52 patients (25.9%), and the high expression group included 149 patients (74.1%). Groups classified according to possession of VNTR, SNP, and low‐ or high‐expression genotypes did not differ significantly in DFS. In multivariate analysis, only tumor stage showed significant prognostic value (hazard ratio (HR) 2.05, 95% CI = 1.24–3.37, P = 0.005).ConclusionsTS polymorphisms do not predict clinical outcome of colorectal cancer patients treated with adjuvant 5‐FU‐based chemotherapy. J. Surg. Oncol. 2010;101:22–27. © 2009 Wiley‐Liss, Inc.
Diagnosis of second head and neck tumors in primary laryngeal SCC is an indicator of overall survival and not associated with poorer overall survival: A single centre study in 987 patients
Authors: Farhadieh Ross Darius , Salardini Arash , Yang Jia Lin , Russell Pamela , Smee Robert 
Published: October 2009

AbstractObjectivesSecond primary tumors (SPTs) have been implicated in poor overall survival (OS) of head and neck squamous cell carcinomas (HNSCCs). Confusion remains regarding their actual incidence and prognostic impact. This study assessed the incidence of SPTs; the SPT diagnostic time lag; the impact on OS; and the mean annual risk.MethodsNine hundred eighty seven consecutive patients treated for primary larynx SCC (1967–2004) were analyzed in this study. 96.3% and 91.4% of patients reached a minimum follow‐up period of 3 and 5 years.ResultsTwo hundred eight (21.1%) patients were diagnosed with SPTs. One hundred forty three (14.5%) patients developed upper aero‐digestive tract (UAD)‐SPTs, 83 (8.4%) were HNSCCs, 56 (5.7%) were lung, and 4 (0.41%) were esophageal‐SPTs. Survival analysis demonstrated clear superior OS rates for the UAD‐SPT (P < 0.008) and HNSCC–SPT (P < 0.001) groups. A comparison of survival of subgroups showed lung/esophagus to have a poorer survival when compared to all other subgroups. OS after diagnosis of an SPT was poorer when compared with no‐SPT group (P < 0.001). The mean annual risk of developing UAD‐SPTs was 2.4%.ConclusionThese results suggest that HNSCC–SPT should not be viewed as an adverse prognostic factor. Reclassifications of UAD‐SPTs into HNSCC–SPT and non‐HNSCC–SPT better reflects their clinical behavior and prognosis. J. Surg. Oncol. 2010;101:72–77. © 2009 Wiley‐Liss, Inc.
The impact of anatomical resection for hepatocellular carcinoma that meets the milan criteria
Authors: Kamiyama Toshiya , Nakanishi Kazuaki , Yokoo Hideki , Kamachi Hirofumi , Matsushita Michiaki , Todo Satoru 
Published: October 2009

AbstractBackgroundThe aim of this study was to analyze the impact of anatomical resection for hepatocellular carcinoma (HCC) that meets the Milan criteria.MethodsBetween 1990 and 2006, 322 consecutive patients with HCC who met the Milan criteria underwent curative resection (R0) and were classified into two groups: Group A (patients with a single HCC, with a tumor diameter of 5 cm or less) and Group B (patients with multiple tumors, no more than three tumor nodules, each with a diameter of 3 cm or less). Patient survival (PS), recurrence‐free survival rates (RFS), and risk factors were analyzed.ResultsUnivariate analysis revealed that in Group A, anatomical resection was the significant factor related to PS, while anatomical resection was that related to RFS. Multivariate analysis showed that in Group A, anatomical resection was a significant favorite factor associated with PS and RFS. Univariate analysis for Group B revealed that anatomical resection was the significant factor related to PS, while that related to RFS was the anatomical resection. Multivariate analysis showed that anatomical resection was a significant favorite factor for only RFS in Group B.ConclusionAnatomical resection improved the surgical outcome of the patients with HCC which met the Milan criteria. J. Surg. Oncol. 2010;101:54–60. © 2009 Wiley‐Liss, Inc.
Liver transplantation versus liver resection for the treatment of hepatocellular carcinoma
Authors: Lee Kyung Keun , Kim Dong Goo , Moon In Sung , Lee Myung Duk , Park Jung Hyun 
Published: October 2009

AbstractPurposeLiver resection (LR) and liver transplantation (LT) are considered the only two potentially curative treatments for hepatocellular carcinoma (HCC). Recently, there has been an intense debate as to whether LR or LT is the optimal initial treatment for patients with Child A or B cirrhosis. The aim of this study was to compare the results of LR and LT in patients with HCC and with Child A or B cirrhosis in a single center over a 10‐year period.MethodSeventy‐eight patients were treated with LT and 130 were treated with LR. We evaluated patient characteristics, short‐term results such as hospital stay, postoperative complication, mortality, and long‐term results such as overall and recurrence‐free survival and recurrence.ResultsThe hospital stay of the LT group was significantly longer than that of the LR group (P < 0.001). The postoperative complication rate and the early operative mortality rate were similar between the two groups. The overall survival rate was higher after LT than it was after LR, but not to a statistically significant degree (P = 0.267). The recurrence‐free survival rate was significantly higher after LT than it was after LR (P = 0.002). Within and beyond the Milan criteria, the overall survival rate was higher after LT than it was after LR, but not to a statistically significant degree. The recurrence‐free survival rate was significantly higher after LT than it was after LR in the patients within Milan criteria (P < 0.001). HCC recurred more frequently after resection (51.5%) than it did after transplantation (29.5%) (P < 0.001), and HCC recurrence developed in the liver more frequently after LR than it did after LT (P = 0.002). However, after recurrence, LR had better survival than LT did, but not to a statistically significant degree (P = 0.177).ConclusionLT should be considered as the primary treatment in patients with HCC within the Milan criteria. LR is recommended for patients with HCC beyond the Milan criteria. The LT group showed a significantly lower recurrence rate than the LR group. However, in the case of recurrence, the LT group showed a poorer long‐term outcome than the LR group. J. Surg. Oncol. 2010;101:47–53. © 2009 Wiley‐Liss, Inc.
Purely vertical upbeat nystagmus in bilateral posterior canal benign paroxysmal positional vertigo: A case report
Authors: Beyea Jason A. , Parnes Lorne S. 
Published: October 2009

AbstractVertical nystagmus occurs in patients with central vestibular system pathology. Lesions of the pons, medulla, and cerebellum lead to vertical nystagmus. Given this association, vertical nystagmus is considered pathognomonic in nature. We present a case of benign paroxysmal positional vertigo (BPPV) with positive Dix‐Hallpike bilaterally, but also with upbeat purely vertical nystagmus in the straight back head hanging position. Computed tomography imaging of the typically pathologic structures in vertical nystagmus (brainstem/posterior fossa) revealed normal anatomy. We propose this case as an instance of peripheral‐associated purely vertical nystagmus without central pathology, while building on our previous understanding of BPPV physiology. Laryngoscope, 2010
Genome‐wide transcriptome analyses reveal p53 inactivation mediated loss of miR‐34a expression in malignant peripheral nerve sheath tumours
Authors: Subramanian Subbaya , Thayanithy Venugopal , West Robert B , Lee Cheng‐Han , Beck Andrew H , Zhu Shirley , Downs‐Kelly Erinn , Montgomery Kelli , Goldblum John R , Hogendoorn Pancras CW , Corless Christopher L , Oliveira Andre M , Dry Sarah M , Nielsen Torsten O , Rubin Brian P , Fletcher Jonathan A , Fletcher Christopher DM , van de Rijn Matt 
Published: October 2009

AbstractMalignant peripheral nerve sheath tumours (MPNSTs) are aggressive soft tissue tumours that occur either sporadically or in patients with neurofibromatosis type 1. The malignant transformation of the benign neurofibroma to MPNST is incompletely understood at the molecular level. We have determined the gene expression signature for benign and malignant PNSTs and found that the major trend in malignant transformation from neurofibroma to MPNST consists of the loss of expression of a large number of genes, rather than widespread increase in gene expression. Relatively few genes are expressed at higher levels in MPNSTs and these include genes involved in cell proliferation and genes implicated in tumour metastasis. In addition, a gene expression signature indicating p53 inactivation is seen in the majority of MPNSTs. Subsequent microRNA profiling of benign and malignant PNSTs indicated a relative down‐regulation of miR‐34a in most MPNSTs compared to neurofibromas. In vitro studies using the cell lines MPNST‐14 (NF1 mutant) and MPNST‐724 (from a non‐NF1 individual) show that exogenous expression of p53 or miR‐34a promotes apoptotic cell death. In addition, exogenous expression of p53 in MPNST cells induces miR‐34a and other miRNAs. Our data show that p53 inactivation and subsequent loss of expression of miR‐34a may significantly contribute to the MPNST development. Collectively, our findings suggest that deregulation of miRNAs has a potential role in the malignant transformation process in peripheral nerve sheath tumours. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Age, stage, and radiotherapy, but not primary tumor site, affects the outcome of patients with malignant rhabdoid tumors
Authors: Sultan Iyad , Qaddoumi Ibrahim , Rodríguez‐Galindo Carlos , Nassan Anwar Al , Ghandour Khalil , Al‐Hussaini Maysa 
Published: October 2009

AbstractBackgroundMalignant rhabdoid tumors (MRTs) are aggressive and often fatal; the Surveillance, Epidemiology, and End Results (SEER) database offers an opportunity to study this rare malignancy.MethodsFrom the SEER database, we extracted records of patients with a reported diagnosis of MRT and analyzed them for clinical features and survival rates by univariate and multivariate analyses.ResultsFor the 229 patients included in our data, who were diagnosed from 1986 to 2005, primary tumors were located in the central nervous system (CNS) (35%), kidneys (20%), and extra‐renal non‐cranial sites (ERNC‐MRTs) (45%). Most patients with renal and CNS tumors were less than 18 years old (87% and 96%, respectively) while more than half of the patients with ERNC‐MRTs (61%) were adults. Among staged tumors, 23% were localized, 34% regional, and 43% distant. Renal tumors had significantly more metastatic disease (47%; P = 0.006) than ERNC‐MRTs. The estimated 5‐year survival for the entire group was 33 ± 3.4% (SE). Univariate and multivariate analyses showed that age at diagnosis (2–18 years), localized stage of tumors, and use of radiotherapy were significantly associated with improved survival. Adults had a better outcome than young children (<2 years old) but a poorer outcome than older children (2–18 years old); tumor stage, but not radiotherapy use, affected outcome in adults. The survival and prognostic factors of children diagnosed before and after 2000 did not differ significantly.ConclusionOur population‐based study indicates that age at diagnosis, tumor stage, and use of radiotherapy favorably impact survival rates of patients with MRTs. Pediatr Blood Cancer 2010; 54:35–40. © 2009 Wiley‐Liss, Inc.
Processing and properties of PLA/thermoplastic starch/montmorillonite nanocomposites
Authors: Arroyo O.H. , Huneault M.A. , Favis B.D. , Bureau M.N. 
Published: October 2009

AbstractThermoplastic starch (TPS) and polylactic acid (PLA) were compounded with natural montmorillonite (MMT) using a twin‐screw extrusion process to investigate the structure and properties of these nanocomposites and to examine the use of water to enhance clay exfoliation. Tensile and essential work of fracture measurements were performed on standard dumbbell shape samples and on double notched samples to determine the effect of MMT and PLA/TPS interfacial modification on the mechanical and fracture properties of the materials. The nanocomposite structure was investigated using X‐Ray diffraction, transmission electron microscopy, and atomic force microscopy. Differential scanning calorimetric analysis was performed on the materials to determine the effect of TPS and MMT on PLA crystallization and physical aging. It was found that the TPS can intercalate the clay structure and that the clay was preferentially located in the TPS phase or at the blend interface. This led to an improvement in tensile modulus and strength and to a reduction in fracture toughness. POLYM. COMPOS., 2010. © 2009 Society of Plastics Engineers
Vibration joining of fiber‐reinforced thermosets
Authors: Beiss T. , Menacher M. , Feulner R. , Huelder G. , Osswald T.A. 
Published: October 2009

AbstractAdhesive bonding is known to be particularly suitable for thermoset composites with continuous fiber reinforcement as it does not interrupt the fibers because of drilled holes. The frequently used two‐part adhesives often require long curing times for the chemical reaction. At the Institute of Polymer Technology (LKT), a vibration‐assisted hot melt bonding technique (vibration joining) was developed, which offers short cycle times and represents a modification of hot melt bonding, using the machine technology from vibration welding. It is suitable to join thermoplastics with thermoset materials or thermosets using a thermoplastic interlayer, by taking advantage of short cycle time and good lap shear strength, compared to bonding with reactive adhesives. Polym Compos 2009 POLYM. COMPOS., 31:1205–1212, 2010. © 2009 Society of Plastics Engineers
The influence of fiber formation conditions on the structure and properties of nanocomposite alginate fibers containing tricalcium phosphate or montmorillonite
Authors: Boguń Maciej , Rabiej Stanisław 
Published: October 2009

AbstractThe authors devised conditions for the formation of nanocomposite calcium alginate fibers containing tricalcium phosphate (TCP) or montmorillonite (MMT). The rheological, sorptive, and strength properties of these fibers, as well as their porous and supramolecular structures were subjected to analysis. It has been concluded that the presence of nanoadditives in the material of alginate fibers decreases their susceptibility to distortion in the drawing stage. The obtained fibers are characterized by an even distribution of the nanoadditive on the fiber surface. POLYM. COMPOS., 31:1321–1331, 2010. © 2009 Society of Plastics Engineers
A “fluorescence switch” technique increases the sensitivity of proteomic detection and identification of S‐nitrosylated proteins
Authors: Tello Daniel , Tarín Carlos , Ahicart Patricia , Bretón‐Romero Rosa , Lamas Santiago , Martínez‐Ruiz Antonio 
Published: October 2009

AbstractProtein S‐nitrosylation is a reversible post‐translational modification of protein cysteines that is increasingly being considered as a signal transduction mechanism. The “biotin switch” technique marked the beginning of the study of the S‐nitrosoproteome, based on the specific replacement of the labile S‐nitrosylation by a more stable biotinylation that allowed further detection and purification. However, its application for proteomic studies is limited by its relatively low sensitivity. Thus, typical proteomic experiments require high quantities of protein extracts, which precludes the use of this method in a number of biological settings. We have developed a “fluorescence switch” technique that, when coupled to 2‐DE proteomic methodologies, allows the detection and identification of S‐nitrosylated proteins by using limited amounts of starting material, thus significantly improving the sensitivity. We have applied this methodology to detect proteins that become S‐nitrosylated in endothelial cells when exposed to S‐nitroso‐L‐cysteine, a physiological S‐nitrosothiol, identifying already known S‐nitrosylation targets, as well as proteins that are novel targets. This “fluorescence switch” approach also allowed us to identify several proteins that are denitrosylated by thioredoxin in cytokine‐activated RAW264.7 (murine macrophage) cells. We believe that this method represents an improvement in order to approach the identification of S‐nitrosylated proteins in physiological conditions.
Tagging of Escherichia coli proteins with new cassettes allowing in vivo systematic fluorescent and luminescent detection, and purification from physiological expression levels
Authors: Wahl Astrid , Hubert Pierre , Sturgis James N. , Bouveret Emmanuelle 
Published: October 2009

AbstractWe designed cassettes allowing the systematic fusion of fluorescent or luminescent proteins preceded by the calmodulin binding peptide tag to the C–terminus of Escherichia coli proteins. The chromosomal insertion, and thus physiological expression level of these fusions, permits the study of protein localization by fluorescent microscopy and protein quantification, in vivo and dynamically in diverse conditions. Furthermore, the calmodulin binding peptide tag allows standard detection, affinity purification, and co–purification experiments. These cassettes are therefore very valuable for the versatility of experiments they make available for a given strain, from biochemistry to dynamic and in vivo studies.