bluuebunny/crossref_metadata_2025_split huggingface dataset:
Procambarus virginalis Lyko, 2017: A new threat to Iberian inland waters
Authors: Sánchez Omar , Oficialdegui Francisco J. , Torralba‐Burrial Antonio , Arbesú Ramón , Valle‐Artaza José María , Fernández‐González Ángel , Ardura Alba , Arias Andrés 
Published: May 2024

AbstractAn eco‐monitoring programme to assess faunal biodiversity in the main rivers of the northern Iberian Peninsula (Spain) reveals the first occurrence of the marbled crayfish Procambarus virginalis (Decapoda: Cambaridae) in Iberian inland waters. Iberian specimens have been identified by combining morphological and genetic traits. We discuss the most plausible pathways and introduction vectors, its potential invasiveness and subsequent impacts on host localities. Our preliminary results raise concern about the potential threat of P. virginalis to native fauna and ecosystem dynamics, as P. virginalis was found in an area of great cultural and ecological importance with relevant populations of endangered species. Due to the invasive history of the marbled crayfish, eradication of these individuals is urgent. This study confirms the importance of early warning systems for exotic species, keeping the population, forest guards and field technicians informed about potential invasive species to execute a rapid and effective response.
As human societies urbanize, so does ecology; taxonomic, geographic, and other research trends in urban vertebrate ecology
Authors: Rainey Travis A. , Schneider Alaini C. , Pakula Carson J. , Swanson Bradley J. 
Published: May 2024

AbstractThe threat to biodiversity posed by urban expansion is well researched and supported. Since the late 1990s, the field of urban ecology has been expanding along with the developed landscapes it studies. Past reviews have shown unequal publication rates in urban ecology literature for taxonomic groups and research locations. Herein, we explore differences in the publication rate of urban studies by vertebrate groups, but also expand on previous investigations by broadening the scope of the literature searched, exploring trends in subtopics within the urban wildlife literature, identifying geographic patterns of such publications, and comparing the rate at which non‐native and threatened and endangered species are studied in urban settings. We used linear and segmented regression to assess publication rates and Fisher's exact tests for comparisons between groups. All vertebrate groups show an increasing proportion of urban studies through time, with urban avian studies accelerating most sharply and herpetofauna appearing to be understudied. Non‐native mammals are more studied than non‐native birds, and threatened and endangered herpetofauna and mammals are more likely to be studied than threatened and endangered birds in urban areas. The plurality of urban wildlife studies are found in North America, while there is a dearth of studies from Africa, Asia, and South America. Our results can help inform decisions of urban ecologists on how to better fill in knowledge gaps and bring a greater degree of equity into the field.
Arbuscular mycorrhizal fungi improve the competitive advantage of a native plant relative to a congeneric invasive plant in growth and nutrition
Authors: Shen Kaiping , He Yuejun , Xia Tingting , Guo Yun , Wu Bangli , Han Xu , Chen Hongchun , Zhao Yan , Wu Pan , Liu Yuan 
Published: May 2024

AbstractPlant invasions severely threaten natural ecosystems, and invasive plants often outcompete native plants across various ecosystems. Arbuscular mycorrhizal (AM) fungi, serving as beneficial microorganisms for host plants, can greatly influence the competitive outcomes of invasive plants against native plants. However, it remains unclear how AM fungi alter the competitive balance between native and invasive species. A competitive experiment was conducted using an invasive Eupatorium adenophorum paired with a native congener Eupatorium lindleyanum. Specifically, both species were inoculated with (M+) or without (M−) the fungus Glomus etunicatum under intraspecific (Intra‐) and interspecific (Inter‐) competition. Plant traits were measured and analyzed regarding the growth and nutrition of both species. The results exhibited that the AM fungus significantly increased the height, diameter, biomass, C, N, and P acquisition of both the invasive E. adenophorum and the native E. lindleyanum. The root mycorrhizal colonization and the mycorrhizal dependency of native E. lindleyanum were greater than those of invasive E. adenophorum. Under M+, the Inter‐competition inhibited the growth and nutrition of invasive E. adenophorum compared to the Intra‐ competition. Further, native E. lindleyanum exhibited higher competitiveness than invasive E. adenophorum in growth and nutrition. Meanwhile, the AM fungus significantly improved the competitiveness of native E. lindleyanum over invasive E. adenophorum. In conclusion, AM fungus improved the competitive advantage of native E. lindleyanum over invasive E. adenophorum in growth and nutrition, potentially contributing to native species competitively resisting the invasion of exotic species. These findings emphasize the importance of AM fungi in helping native plants resist the invasion of exotic plants and further contribute to understanding plant invasion prevention mechanisms.
2‐ and 3‐Fluorocyclobutane Building Blocks for Organic and Medicinal Chemistry
Authors: Malashchuk Andrii , Demchuk Oleksandr P. , Razhyk Bohdan V. , Holumbievskyi Vitalii O. , Kudryk Oleksandr V. , Chernykh Anton V. , Hryshchuk Oleksandr V. , Sosunovych Bohdan S. , Volochnyuk Dmytro M. , Vashchenko Bohdan V. , Grygorenko Oleksandr O. 
Published: May 2024

AbstractMultigram synthesis of 3‐fluorinated cyclobutane building blocks has been disclosed. The proposed synthetic schemes were based on nucleophilic fluorination and eventually led to 3‐fluorocyclocutanecarboxylic acid as a key target compound and intermediate. Further functional group transformations provided monofluorinated cyclobutane‐derived alcohols, amines, bromides, thiols, sulfonyl chlorides, etc. Furthermore, the synthesis of diastereopure cis‐ and trans‐isomeric 2‐ and 3‐fluorocyclobutanecarboxylic acids and amines was also performed. In both cases, diastereomer separation was used to obtain pure stereoisomers. The effect of the fluorine position and relative spatial orientation on the physicochemical properties of the corresponding monoflurinated derivatives was studied by measurements of pKa and logP values.
Characterization of the transcriptional effects of the plastic additive dibutyl phthalate alone and in combination with microplastic on the green-lipped mussel Perna canaliculus
Authors: Baettig Camille G. , Laroche Olivier , Ockenden Amy , Smith Kirsty F. , Lear Gavin , Tremblay Louis A. 
Published: May 2024

Abstract
The presence and persistence of microplastics (MPs) in diverse aquatic environments are of global concern. Microplastics can impact marine organisms via direct physical interaction and the release of potentially harmful chemical additives incorporated into the plastic. These chemicals are physically bound to the plastic matrix and can leach out. The hazards associated with chemical additives to exposed organisms is not well characterized. We investigated the hazards of plastic additives leaching from plastic. We used the common plasticizer dibutyl phthalate (DBP) as a chemical additive proxy and the New Zealand green-lipped mussel (Perna canaliculus) as a model. We used early-adult P. canaliculus exposed to combinations of virgin and DBP-spiked polyvinyl chloride (PVC), MPs, and DBP alone for 7 days. Whole transcriptome sequencing (RNA-seq) was conducted to assess whether leaching of DBP from MPs poses a hazard. The differences between groups were evaluated using pairwise permutational multivariate analysis of variance (PERMANOVA), and all treatments were significantly different from controls. In addition, a significant difference was seen between DBP and PVC MP treatment. Transcriptome analysis revealed that mussels exposed to DBP alone had the most differentially expressed genes (914), followed by PVC MP + DBP (448), and PVC MP (250). Gene ontology functional analysis revealed that the most enriched pathway types were in cellular metabolism, immune response, and endocrine disruption. Microplastic treatments enriched numerous pathways related to cellular metabolism and immune response. The combined exposure of PVC MP + DBP appears to cause combined effects, suggesting that DBP is bioavailable to the exposed mussels in the PVC MP + DBP treatment. Our results support the hypothesis that chemical additives are potentially an important driver of MP toxicity. Environ Toxicol Chem 2024;43:1604–1614. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Synergies of storing hydrogen at the crest of CO2${\rm CO}_{2}$ or other gas storage
Authors: Rhouma Sabrine Ben , Chabab Salaheddine , Broseta Daniel 
Published: May 2024

AbstractThere are mutual benefits in storing in sedimentary reservoirs jointly with another gas serving as a cushion gas, such as the of a carbon capture and storage (CCS) operation or the natural gas of seasonal storage or left in a depleted hydrocarbon reservoir. When occupies the crest of the reservoir, the presence of either gas is beneficial to the other. reinforces the sealing efficiency of the caprock due to its very favorable interfacial properties with respect to brine and rock‐forming minerals. storage safety and capacity are also increased with cushion gases such as , which alleviate the buoyancy pressure at the top of the gas column. The potential drawback of this storage scheme is gas/gas mixing, which can, however, be strongly reduced if, by an appropriate choice of well completion and placement, is positioned in the upper zones of the reservoir, and its injection rate is kept below a critical value corresponding to the incipient fingering instability of the gas mixing zone. This value, which depends on reservoir permeability, the dip angle of the mixing front, and how density varies with viscosity in the mixing front, turns out to be well above practical injection rates. Therefore, dispersive mixing is the only cause of front spreading, which is acceptable for not‐too‐heterogeneous reservoirs. The mutual benefits identified in this study are the strongest when the cushion is made up of dense , which suggests that the crest of offshore storage reservoirs is a good candidate for storage. © 2024 The Authors.Greenhouse Gases: Science and Technologypublished by Society of Chemical Industry and John Wiley & Sons Ltd.
Outcomes of internal mammary artery perforator flap in head and neck reconstruction: A systematic review
Authors: Vasudevan Srivatsa Surya , Rogers Brianna , Adilbay Dauren , Olinde Lindsay , Pang John , Nathan Cherie‐Ann O. , Asarkar Ameya A. 
Published: May 2024

AbstractThis study aims to evaluate the functional and prognostic outcomes associated with the internal mammary artery perforator (IMAP) flap in various head and neck defect repairs, given the current lack of clarity on its effectiveness. We performed a systematic review of various databases: PubMed, Embase, Scopus, Web of Science, and ScienceDirect using keywords such as “Internal mammary artery perforator flap” and “IMAP.” Screening and data extractions were performed by two individual reviewers. Articles were considered eligible if they included sufficient information on IMAP flap features, their applications in the head and neck, and outcomes. From 264 articles analyzed, 24 studies were included for qualitative analysis. Out of which, 125 patients who received internal mammary artery perforator flaps were included. Most of the patients, 103 (88%), received pedicled IMAP flaps, and 22 (12%) received IMAP free flaps. The second internal mammary artery (IMA) was favored as the single perforator (81.5%), with the combination of the first and second IMA being the primary choice for dual perforators (92.5%). IMAP flaps were predominantly single perforator flaps (65%), with 35% being dual perforator flaps. Among various applications, IMAP flaps are commonly employed in the reconstruction of neck defects (25.5%), pharyngocutaneous fistula repair (20.8%), and burn scar contracture restoration (8%). Only seven (5.6%) patients had flap complications, including venous congestion (1.6%), partial necrosis (1.6%), complete necrosis (1.6%), and incision dehiscence (0.8%). Donor sites were predominantly closed by the primary closure (92%). 3.2% of donor sites had minor complications. The average follow‐up was 12.6 (IQR: 6–18) months. This systematic review highlights the effectiveness and safety of IMAP flaps in head and neck reconstruction, with positive outcomes and minimal complications.
An integrated classification of tumor suppressor IKZF1 inactivation and oncogenic activation in Philadelphia chromosome‐like acute lymphoblastic leukemia
Authors: Huang Zicong , Zhang Ling , Gong Xiaoyuan , Li Jia , Deng Shiyu , Cai Zihong , Tang Bingqing , Huang Kangyu , Li Xin , Zhao Weihua , Xu Yang , Xuan Li , Liu Qifa , Wang Ying , Chen Suning , Zhou Hongsheng 
Published: May 2024

AbstractPhiladelphia chromosome (Ph)‐like acute lymphoblastic leukemia (ALL) is recognized for its genetic and clinical diversity. In this study, we identified a novel high‐risk subset of Ph‐like ALL, characterized by the activation of oncogenic signaling and the inactivation of the tumor suppressor gene IKZF1, resulting in a dismal outcome. The association between cytogenetic aberrations and clinical features was assessed on a cohort of 191 patients with Ph‐like ALL. Our findings revealed that patients with inactivation of IKZF1 combined with activation of oncogenic signaling (CRLF2/EPOR/JAK2 rearrangements or p‐CRKL/p‐STAT5 high expression) had the worst outcome (3‐year overall survival [OS] of 28.8% vs. 80.1% for others, p < 0.001; 2‐year event‐free survival [EFS] of 6.5% vs. 57.0% for others, p < 0.001). Multivariable analysis demonstrated that this high‐risk feature was an independent inferior prognostic factor (adjusted hazard ratio for OS = 4.55, 95% confidence interval [CI]: 2.35–8.81, p < 0.001; adjusted hazard ratio for EFS = 3.27, 95% CI: 1.99–5.39, p < 0.001). Allogeneic hematopoietic stem cell transplantation was associated with improved prognoses in patients within the high‐risk subgroup. In conclusion, this study identified a clinically distinct entity that possesses effective prognostic features and provides potential guidance for refining risk stratification in Ph‐like ALL.
Free convective heat transfer induced inside a vented duct having two aligned hot and cold cylinders: An experimental study
Authors: Alomar Omar Rafae , Ali Omar Mohammed , Al‐Omar Shaswar Omar Osman 
Published: May 2024

AbstractFree convective heat transfer created from two aligned cylinders immersed inside a vented air duct is experimentally investigated. The experiments include the measurements of cylinders temperature and the air temperature inside the enclosure under steady, turbulent, and incompressible flow properties by using steady‐state heat equations. The studied parameters include Rayleigh number (), opening sizes at lower and upper enclosure surfaces (), and space size between cylinders () with the constant ratio of enclosure width to cylinder diameter equal to 6. The findings displayed that the average air temperature inside the enclosure for the low values of S and O is low, and it rises as S is raised. The behaviors of Nu differ with changing Ra values. An interaction between hot cylinder and cold cylinder inside the enclosure is observed that depends on O and S values, and hence, they have a large impact on fluid temperature. The data indicated that rises with the for all and values. The value of for each cylinder depends on the values of and . The maximum value has been obtained when and with maximum enhancement between 30% and 50% depending on Ra values as compared with and , whereas the minimum average has been indicated when and . The profiles of reveal that , , and have a considerable influence on heat transfer.
Seminoma in a 46, XY patient with 17α‐hydroxylase deficiency
Authors: Maekawa Ken , Shimizu Yousuke , Hayashi Koken , Hatano Shotaro , Miyauchi Yasuyuki , Sakurai Takaki , Mitsumori Kenji , Onishi Hiroyuki 
Published: May 2024

Introduction17α‐Hydroxylase deficiency is a very rare disease reported to be associated with a risk of gonadal malignancy. We herein report a rare case of seminoma in a 46, XY patient with 17α‐hydroxylase deficiency.Case presentationA 52‐year‐old woman presented with a 9‐cm pelvic tumor. At age 14, she had been identified as having the XY karyotype and 17α‐hydroxylase deficiency. However, she was not informed and did not consult the urology department. Laparoscopic gonadectomy was performed at the latest consultation, and seminoma was diagnosed.ConclusionThis is the third reported case of testicular tumor and the first of germ cell tumor in a 46, XY patient with 17α‐hydroxylase deficiency. Given the rarity and the risk of gonadal malignancy associated with 17α‐hydroxylase deficiency, the involvement of multidisciplinary specialists and prophylactic gonadectomy is considered crucial in its management.
Longitudinal effects of sexual minority stigma on depressive symptoms among young men who have sex with men: Distinguish between and within person effects
Authors: Liu Xin , Zhai Mengxi , Li Jiayu , Liu Dan , Yuan Liang , Wang Huihao , Yu Bin , Yan Hong 
Published: May 2024

AbstractBackgroundYoung men who have sex with men (YMSM) may experience high levels of sexual minority stigma (SMS) and depressive symptoms (DS) over the world and in China. However, there is a lack of studies investigating the longitudinal effects of SMS on DS of YMSM, especially focusing on YMSM and separating the between‐person and within‐person effects. This study aimed to fill the said gaps.MethodsStudy data were derived from a prospective cohort of 349 YMSM from central China (Wuhan, Changsha, Nanchang), the baseline survey was started in 2017 with one follow‐up visit every year. SMS and DS were measured three times using valid and reliable instruments. The cross‐lagged panel model (CLPM) and the random intercept CLPM (RI‐CLPM) were used to examine the between‐person and within‐person concurrent and lagged effects, respectively.ResultsFindings of CLPM revealed bidirectional associations between SMS and DS over time. RI‐CLPM suggested that at the between‐person level, SMS was significantly associated with DS, echoing the results of CLPM. However, this reciprocal relationship has not been found at the within‐person level.ConclusionThe associations between SMS and DS among YMSM at the population level is more significant than that at the individual level. We suggest that interventions should be against the adverse effects of cultural marginalization and systemic change the social concepts to reduce the amount of SMS in society.
SOAT1 in gallbladder cancer: Clinicopathological significance and avasimibe therapeutics
Authors: Hong Yuqun , Abudukeremu Xiahenazi , She Feifei , Chen Yanling 
Published: May 2024

AbstractThe aim of this investigation was to evaluate the differential expression of the sterol O‐acyltransferase 1 (SOAT1) protein in gallbladder cancer tissues and cells, investigate the impact of Avastin on the proliferation, migration, invasion capabilities of gallbladder cancer cells, and its potential to induce cell apoptosis. Immunohistochemical analysis of samples from 145 gallbladder cancer patients was conducted, along with analysis of SOAT1 protein, mRNA expression levels, and cholesterol content in gallbladder cancer cell lines SGC‐996, NOZ, and gallbladder cancer (GBC)‐SD using Western blot and q‐PCR techniques. Furthermore, the effects of Avastin on the proliferation, migration, and invasion capabilities of these gallbladder cancer cell lines were studied, and its ability to induce cell apoptosis was evaluated using flow cytometry, Western blot, and immunohistochemical methods. Additionally, gene expression and pathway analysis were performed, and the synergistic therapeutic effects of Avastin combined with gemcitabine were tested in a gallbladder cancer xenograft model. The study found that SOAT1 expression was significantly upregulated in GBC tissues and positively correlated with lymph node metastasis and TNM staging. In vitro experiments demonstrated that Avastin significantly inhibited the proliferation, migration, and invasion capabilities of SGC‐996 and GBC‐SD cell lines and induced apoptosis. RNA sequencing analysis revealed multiple differentially expressed genes in cells treated with Avastin, primarily enriched in biological pathways such as signaling transduction, malignant tumors, and the immune system. In vivo, experiments confirmed that Avastin could effectively suppress tumor growth in a gallbladder cancer xenograft model and enhanced the treatment efficacy when used in combination with gemcitabine. Overall, these findings provide new insights and strategies for targeted therapy in gallbladder cancer.
Tumor formation at ileocecal junction associated with interleukin‐1β upregulation in aryl hydrocarbon receptor‐deficient mouse
Authors: Ikuta Togo , Kanda Hiroaki 
Published: May 2024

AbstractAryl hydrocarbon receptor (AhR) is a ligand‐dependent transcription factor. We previously reported spontaneous ileocecal tumorigenesis in AhR‐deficient mice after the age of 10 weeks, which originated in the confined area between ileum and cecum. This study aimed to investigate the underlying mechanism that causes tumor development at this particular location. To observe mucosal architecture in detail, tissues of ileocecal region were stained with methylene blue. Gene expression profile in the ileocecal tissue was compared with cecum. Immunohistochemical analysis was performed with ileocecal tissues using antibodies against ileum‐specific Reg3β or cecum‐specific Pitx2. In AhR+/+ mice and AhR+/− mice, that do not develop lesions, methylene blue staining revealed the gradually changing shape and arrangement of villi from ileum to cecum. It was also observed in AhR‐deficient mice before developing lesions. Microarray‐based analysis revealed abundant antimicrobial genes, such as Reg3, in the ileocecal tissue while FGFR2 and Pitx2 were specific to cecum. Immunohistochemical analysis of AhR‐deficient mice indicated that lesions originated from the ileocecal junction, a boundary area between different epithelial types. Site‐specific gene expression analysis revealed higher expression of IL‐1β at the ileocecal junction compared with the ileum or cecum of 9–11‐week‐old AhR‐deficient mice. These findings indicate that AhR plays a vital function in the ileocecal junction. Regulating AhR activity can potentially manage the stability of ileocecal tissue possessing cancer‐prone characteristics. This investigation contributes to understanding homeostasis in different epithelial transitional tissues, frequently associated with pathological states.
Multifold 1‐perfect codes
Authors: Krotov Denis S. 
Published: May 2024

AbstractA multifold 1‐perfect code (1‐perfect code for list decoding) in any graph is a set of vertices such that every vertex of the graph is at distance not more than 1 from exactly elements of . In ‐ary Hamming graphs, where is a prime power, we characterize all parameters of multifold 1‐perfect codes and all parameters of additive multifold 1‐perfect codes. In particular, we show that additive multifold 1‐perfect codes are related to special multiset generalizations of spreads, multispreads, and that multispreads of parameters corresponding to multifold 1‐perfect codes always exist.