bluuebunny/crossref_metadata_2025_split huggingface dataset:
Premenstrual syndrome as reported by Brazilian women
Authors: Petta Carlos A. , Osis Maria J. Duarte , de Pádua Karla S. , Bahamondes Luis , Makuch María Y. 
Published: November 2009

AbstractObjectiveTo describe the perspectives and attitudes of Brazilian women toward premenstrual syndrome (PMS).MethodsAn exploratory study was conducted in 6 major cities: 1 in each geographic region of Brazil and 1 in the Federal District. Participants were women aged 18–40 years who consulted at public healthcare services or who were members of staff/faculty at university.ResultsOf 1053 women, 96.1% had heard of PMS; 65.4% considered that all or almost all women experienced the condition; 87.5% stated that symptoms occurred prior to menstruation; and 60.3% thought that they had PMS at the time of the interview. The emotional and physical symptoms most frequently mentioned were nervousness/anxiety (76.4%); mood swings/crying (55.7%); pain, swelling, and tenderness of the breasts (37.3%); and cramps (45.4%).ConclusionPremenstrual syndrome was reported by a large number of Brazilian women; actions need to be taken to provide more effectively the specific information required by both lay people and healthcare professionals.
Variations in the mitochondrial cytochrome c oxidase subunit I gene indicate northward expanding populations ofCulicoides imicolain Spain
Authors: Calvo J.H. , Calvete C. , Martinez-Royo A. , Estrada R. , Miranda M.A. , Borras D. , Sarto I Monteys V. , Pages N. , Delgado J.A. , Collantes F. , Lucientes J. 
Published: November 2009

AbstractCulicoides imicolais the main vector for bluetongue (BT) and African horse sickness (AHS) viruses in the Mediterranean basin and in southern Europe. In this study, we analysed partial mitochondrial cytochrome c oxidase subunit I (COI) gene to characterize and confirm population expansion ofCulicoides imicolaacross Spain. The data were analysed at two hierarchical levels to test the relationship betweenC. imicolahaplotypes in Spain (n=215 from 58 different locations) and worldwide (n=277). We found nineteen different haplotypes within the Spanish population, including 11 new haplotypes. No matrilineal subdivision was found within the Spanish population, while western and eastern MediterraneanC. imicolapopulations were very structured. These findings were further supported by median networks and mismatch haplotype distributions. Median networks demonstrated that the haplotypes we observed in the western Mediterranean region were closely related with one another, creating a clear star-like phylogeny separated only by a single mutation from eastern haplotypes. The two, genetically distinct, sources ofC. imicolain the Mediterranean basin, thus, were confirmed. This type of star-like population structure centred around the most frequent haplotype is best explained by rapid expansion. Furthermore, the proposed northern expansion was also supported by the statistically negative Tajima's D and Fu's Fs values, as well as predicted mismatch distributions of sudden and spatially expanding populations. Our results thus indicated thatC. imicolapopulation expansion was a rapid and recent phenomenon.
Parents' use of conventional and unconventional labels in conversations with their preschoolers
Authors: HENDERSON ANNETTE M. E. , SABBAGH MARK A. 
Published: November 2009

ABSTRACTParents' use of conventional versus unconventional labels with their two- (n=12), three- (n=12) and four-year-old children (n=12) was assessed as they talked about objects that were either known or unknown to them. For known objects, parents provided typical conventional labels casually during the conversation. For unknown objects, parents were less likely to use typical nouns as labels and marked their labels with additional information suggesting that the labels might be unconventional. Parents marked potentially unconventional labels by providing explicit statements of ignorance and paralinguistic cues of uncertainty. These patterns were strongest when the unknown objects were manufactured as opposed to homemade, possibly because manufactured objects are supposed to have conventional names that parents were unable to provide. Parents' marking of unconventional labels may help children recognize when new word forms should be treated with caution and guide their learning accordingly.
Assembly of an Interactive Correlation Network for the Arabidopsis Genome Using a Novel Heuristic Clustering Algorithm
Authors: Mutwil Marek , Usadel Bjoݶrn , Schuݶtte Moritz , Loraine Ann , Ebenhoݶh Oliver , Persson Staffan 
Published: November 2009

Abstract
A vital quest in biology is comprehensible visualization and interpretation of correlation relationships on a genome scale. Such relationships may be represented in the form of networks, which usually require disassembly into smaller manageable units, or clusters, to facilitate interpretation. Several graph-clustering algorithms that may be used to visualize biological networks are available. However, only some of these support weighted edges, and none provides good control of cluster sizes, which is crucial for comprehensible visualization of large networks. We constructed an interactive coexpression network for the Arabidopsis (Arabidopsis thaliana) genome using a novel Heuristic Cluster Chiseling Algorithm (HCCA) that supports weighted edges and that may control average cluster sizes. Comparative clustering analyses demonstrated that the HCCA performed as well as, or better than, the commonly used Markov, MCODE, and k-means clustering algorithms. We mapped MapMan ontology terms onto coexpressed node vicinities of the network, which revealed transcriptional organization of previously unrelated cellular processes. We further explored the predictive power of this network through mutant analyses and identified six new genes that are essential to plant growth. We show that the HCCA-partitioned network constitutes an ideal “cartographic” platform for visualization of correlation networks. This approach rapidly provides network partitions with relative uniform cluster sizes on a genome-scale level and may thus be used for correlation network layouts also for other species.
The Nuclear Interactor PYL8/RCAR3 ofFagus sylvaticaFsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress
Authors: Saavedra Xandra , Modrego Abelardo , Rodriݩguez Dolores , Gonzaݩlez-Garciݩa Mary Paz , Sanz Luis , Nicolaݩs Gregorio , Lorenzo Oscar 
Published: November 2009

AbstractThe functional protein phosphatase type 2C from beechnut (Fagus sylvatica; FsPP2C1) was a negative regulator of abscisic acid (ABA) signaling in seeds. In this report, to get deeper insight on FsPP2C1 function, we aim to identify PP2C-interacting partners. Two closely related members (PYL8/RCAR3 and PYL7/RCAR2) of the Arabidopsis (Arabidopsis thaliana) BetV I family were shown to bind FsPP2C1 in a yeast two-hybrid screening and in an ABA-independent manner. By transient expression of FsPP2C1 and PYL8/RCAR3 in epidermal onion (Allium cepa) cells and agroinfiltration in tobacco (Nicotiana benthamiana) as green fluorescent protein fusion proteins, we obtained evidence supporting the subcellular localization of both proteins mainly in the nucleus and in both the cytosol and the nucleus, respectively. The in planta interaction of both proteins in tobacco cells by bimolecular fluorescence complementation assays resulted in a specific nuclear colocalization of this interaction. Constitutive overexpression of PYL8/RCAR3 confers ABA hypersensitivity in Arabidopsis seeds and, consequently, an enhanced degree of seed dormancy. Additionally, transgenic 35S:PYL8/RCAR3 plants are unable to germinate under low concentrations of mannitol, NaCl, or paclobutrazol, which are not inhibiting conditions to the wild type. In vegetative tissues, Arabidopsis PYL8/RCAR3 transgenic plants show ABA-resistant drought response and a strong inhibition of early root growth. These phenotypes are strengthened at the molecular level with the enhanced induction of several ABA response genes. Both seed and vegetative phenotypes of Arabidopsis 35S:PYL8/RCAR3 plants are opposite those of 35S:FsPP2C1 plants. Finally, double transgenic plants confirm the role of PYL8/RCAR3 by antagonizing FsPP2C1 function and demonstrating that PYL8/RCAR3 positively regulates ABA signaling during germination and abiotic stress responses.
A randomized controlled trial comparing two different dosages of infusional pantoprazole in peptic ulcer bleeding
Authors: Hsu Yao‐Chun , Perng Chin‐Lin , Yang Tzeng‐Huey , Wang Chaur‐Shine , Hsu Wei‐Lun , Wu Huei‐Tang , Cheng Yang‐Chih , Chiang Ming‐Feng , Lin Hwai‐Jeng 
Published: November 2009

WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT • An adjunct to endoscopy, proton pump inhibitor (PPI) is effective pharmacotherapy in high‐risk patients with peptic ulcer bleeding (PUB).• An intravenous 80‐mg bolus and 192 mg day−1 successive infusion for 3 days is the currently recommended dosing modality in administering PPI. WHAT THIS STUDY ADDS • Clinical outcomes are not different in PUB patients receiving infusional pantoprazole at either 192 mg or 160 mg day−1 for 3 days.• The effective dosage of PPI may not be as high as currently recommended.• In view of cost‐effectiveness, a lower dosage (160 mg day−1) of infusional PPI may be adopted in the management of PUB.AIM The optimal dosage of proton pump inhibitor in bleeding peptic ulcers remains controversial.The aim was to compare the clinical effectiveness of two doses of infusional pantoprazole in peptic ulcer bleeding.METHODS Peptic ulcer patients (n= 120) with bleeding stigmata were enrolled after successful endoscopic therapy. After an initial bolus injection of 80 mg pantoprazole, patients were randomized to receive continuously infused pantoprazole at either 192 mg day−1 or 40 mg every 6 h (i.e. 160 mg day−1) for 3 days. Clinical outcomes between the two groups within 14 days were compared, with 14‐day recurrent bleeding regarded as the primary end‐point.RESULTS Both groups (n= 60 each) were well matched in demographic and clinical factors upon entry. Bleeding totally recurred in 11 (9.2%) patients, with six (10%) in the 192 mg day−1 group and five (8.3%) in the 160 mg day−1 group (relative risk of bleeding recurrence between two treatments 1.2; 95% CI 0.39, 3.72). All secondary outcomes between the two groups were similar, including the amount of blood transfusion (mean 1179 ml vs. 1203 ml, P > 0.1), hospital stay (mean 9.5 days vs. 9.9 days, P > 0.1), need for surgery (n= 1 vs. n= 0, P > 0.1), and mortality (n= 1 vs. n= 0, P > 0.1).CONCLUSIONS Following endoscopic haemostasis, infusional pantoprazole at either 192 mg day−1 or 40 mg every 6 h appear similar.
Pseudo‐placentational Endometrial Cysts in a Bitch
Authors: Bartel C. , Schönkypl S. , Walter I. 
Published: November 2009

SummaryCystic alterations of the canine endometrium compromise reproduction and fertility of the bitch and may lead to life‐threatening diseases, such as pyometra. Even without clinical evidence, reduction of the uterine lumen by cysts implicates disturbances during migration, nidation and development of the embryo. Several studies point to the high variability of morphology of uterine endometrial cysts but they lack detailed analyses of alterations. In the present study, immunohistochemistry was used to investigate the expression of steroid hormone receptors (oestrogen, progesterone), proliferation activity, inflammation and infection in the cystic affected tissue regions in contrast to the normal endometrium. Oestrogen receptor expression showed a high density of receptors throughout the surface epithelial cells, crypt epithelial cells, glandular epithelial cells and stromal cells of the normal endometrium as well as the cystic affected regions. Proliferation in the cysts was verified in the middle and basal cells of the crypts. Neither in the endometrium nor in the cysts inflammatory processes or evidence of infection could be detected. Furthermore, lectin histochemistry and electron microscopic methods showed that lectin binding patterns and cell morphology of internal epithelial lining and surface epithelium of the cysts can be used to characterize and distinguish different types of cystic alterations. Analogies between epithelial cells of the glandular chambers of the canine placenta and the cystic cellular morphology, steroid hormone receptor distribution as well as lectin binding patterns of the endometrial cysts, as observed in this study, suggest to introduce the term ‘pseudo‐placentational endometrial cysts’.
Aerosolized vancomycin for the treatment of MRSA after lung transplantation
Authors: HAYES JR Don , MURPHY Brian S. , MULLETT Timothy W. , FEOLA David J. 
Published: November 2009

ABSTRACTLung transplantation is the only life‐prolonging therapy available for cystic fibrosis (CF) patients with end‐stage lung disease. The presence of pathogens in the airways of CF patients prior to transplantation is the major risk factor for infection in the post‐transplantation period, with methicillin‐resistant Staphylococcus aureus (MRSA) having a growing impact. Aerosolized vancomycin has been used successfully in the treatment of MRSA in the CF population but its use after lung transplantation has not been previously reported. We report the case of a lung transplant recipient who was successfully treated for MRSA infection with aerosolized vancomycin.
The reverse operation of Na+/Cl−‐coupled neurotransmitter transporters – why amphetamines take two to tango
Authors: Sitte Harald H. , Freissmuth Michael 
Published: November 2009

AbstractSodium‐chloride coupled neurotransmitter transporters achieve reuptake of their physiological substrate by exploiting the pre‐existing sodium‐gradient across the cellular membrane. This terminates the action of previously released substrate in the synaptic cleft. However, a change of the transmembrane ionic gradients or specific binding of some psychostimulant drugs to these proteins, like amphetamine and its derivatives, induce reverse operation of neurotransmitter:sodium symporters. This effect eventually leads to an increase in the synaptic concentration of non‐exocytotically released neurotransmitters [and – in the case of the norepinephrine transporters, underlies the well‐known indirect sympathomimetic activity]. While this action has long been appreciated, the underlying mechanistic details have been surprisingly difficult to understand. Some aspects can be resolved by incorporating insights into the oligomeric nature of transporters, into the nature of the accompanying ion fluxes, and changes in protein kinase activities.
Endothelium modulates vasoconstrictor response to prostaglandin I2 in rat mesenteric resistance arteries: interaction between EP1 and TP receptors
Authors: Xavier FE , Blanco‐Rivero J , Ferrer M , Balfagón G 
Published: November 2009

Background and purpose:  Prostacyclin (PGI2) is usually described as an endothelium‐derived vasodilator, but it can also induce vasoconstriction. We studied the vasomotor responses to PGI2 in resistance arteries and the role of thromboxane (TP) and prostaglandin E2 (EP) receptors in this effect.Experimental approach:  Mesenteric resistance arteries were obtained from Sprague‐Dawley rats. Vasomotion to PGI2 was studied in segments of these arteries with and without endothelium and in presence of the nitric oxide (NO) synthase inhibitor NG‐nitro‐L‐arginine methyl ester (L‐NAME), the potassium channel blockers apamin plus charybdotoxin, the non‐selective EP receptor antagonist AH6809, the selective TP receptor antagonist SQ29548 or the EP1 receptor antagonist SC19220. PGI2‐induced NO release was analysed in the absence or presence of SQ29548, AH6809 or SC19220.Key results:  PGI2 caused contractions in arterial segments that were increased by endothelium removal, L‐NAME or L‐NAME plus apamin plus charybdotoxin and abolished by SQ29548. In segments with endothelium, AH6809 or SC19220 almost abolished the contractions to PGI2; this effect was prevented by L‐NAME, L‐NAME plus apamin plus charybdotoxin or by endothelium removal. PGI2 induced NO release that was inhibited by the prostacyclin receptor (IP receptor) antagonist, RO1138452, and increased by SQ29548, SC19220 and AH6809. The increase in NO release induced by these separate drugs was inhibited by RO1138452.Conclusions and implications:  PGI2 activated the TP receptor in mesenteric resistance arteries and produced vasoconstriction, which the endothelium modulated through TP and EP1 receptors. PGI2 also released endothelium‐derived hyperpolarizing factor and, through IP receptor activation, induced NO release, which in turn, was antagonized by TP and EP1 receptor activation.
Antifungal Susceptibility of 205
Candida
spp. Isolated Primarily during Invasive Candidiasis and Comparison of the Vitek 2 System with the CLSI Broth Microdilution and Etest Methods
Authors: Bourgeois N. , Dehandschoewercker L. , Bertout S. , Bousquet P.-J. , Rispail P. , Lachaud L. 
Published: November 2009

ABSTRACT

Infections due to
Candida
spp. are frequent, particularly in immunocompromised and intensive care unit patients. Antifungal susceptibility tests are now required to optimize antifungal treatment given the emergence of acquired antifungal resistance in some
Candida
species. An antifungal susceptibility automated method, the Vitek 2 system (VK2), was evaluated. VK2 was compared to the CLSI broth microdilution reference method and the Etest procedure. For this purpose, 205 clinical isolates of
Candida
spp., including 11 different species, were tested for fluconazole, voriconazole, and amphotericin B susceptibility. For azoles, essential agreement ranged from 25% to 100%, depending on the method used and the
Candida
species tested. Categorical agreements for all of the species averaged 92.2% and ranged from 14.3 to 100%, depending on the 24-h or 48-h MIC reading by the Etest and CLSI methods and on the
Candida
species. Results obtained for
Candida albicans
showed excellent categorical and essential agreements with the two comparative methods. For
Candida glabrata
, the essential agreement was high with the CLSI method but low with the Etest method, and several very major errors in interpretation were observed between VK2 and the Etest method for both azoles. Low MICs of fluconazole were obtained for all of the
Candida krusei
isolates, but the VK2 expert software corrected all of the results obtained to resistant. Amphotericin B results showed MICs of ≤1 mg/liter for 201 (VK2), 190 (CLSI), and 202 (Etest) isolates. The AST-YS01 Vitek 2 card system (bioMérieux) is a reliable and practical standardized automated antifungal susceptibility test. Nevertheless, more assays are needed to better evaluate
C
.
glabrata
fluconazole sensitivity.
Detection of CTX-M-Type Extended-Spectrum Beta-Lactamase (ESBLs) by Testing with MicroScan Overnight and ESBL Confirmation Panels
Authors: Jorgensen James H. , McElmeel M. L. , Fulcher L. C. , Zimmer B. L. 
Published: November 2009

ABSTRACT

CTX-M extended-spectrum beta-lactamases (ESBLs) have emerged as the most common type of ESBL globally, their incidence easily surpassing those of SHV and TEM ESBLs in most locales. This study compared the performance of two MicroScan dried panels with CLSI reference broth microdilution and disk diffusion methods on a collection of genetically characterized ESBL-producing isolates. These included 64
Enterobacteriaceae
isolates that produced CTX-M8, -14, -15, or -16 according to PCR and sequencing of the
bla
gene, 17 isolates that produced a SHV or TEM ESBL, and 19 that produced both CTX-M and SHV ESBLs. Each isolate was tested by a frozen reference microdilution panel, the MicroScan ESβL
plus
confirmation panel, and a routine dried panel containing streamlined ESBL confirmation dilutions (MicroScan Neg MIC panel type 32) that included cefotaxime and ceftazidime tested alone or with a fixed concentration of 4 μg/ml of clavulanate. Each isolate was also tested by the standard CLSI double-disk confirmation tests. The disk diffusion method detected all ESBL-producing isolates, the frozen reference panel detected 90% of isolates (10 out of 100 could not be analyzed because of off-scale MICs that exceeded the clavulanate combination concentrations in the panel), the ESβL
plus
panel detected 98% (1 missed and 1 off scale), and the streamlined ESBL panel detected 95% (5 off scale). Very high MICs for a few strains that produced SHV or both CTX-M and SHV ESBLs precluded noting the required three twofold-dilution differences with clavulanate needed to confirm an ESBL primarily in the reference panel and the Neg type 32 panel.
Neosartorya udagawae
(
Aspergillus udagawae
), an Emerging Agent of Aspergillosis: How Different Is It from
Aspergillus fumigatus
?
Authors: Sugui J. A. , Vinh D. C. , Nardone G. , Shea Y. R. , Chang Y. C. , Zelazny A. M. , Marr K. A. , Holland S. M. , Kwon-Chung K. J. 
Published: November 2009

ABSTRACT

A recent report on several cases of invasive aspergillosis caused by
Neosartorya udagawae
suggested distinctive patterns of disease progression between
N. udagawae
and
Aspergillus fumigatus
. This prompted us to characterize
N. udagawae
in comparison to
A. fumigatus
. Our findings showed that both species exist in two mating types at similar ratios and produce gliotoxin. However, the thermotolerance of the two species differs: while
A. fumigatus
is able to grow at 55°C but not at 10°C,
N. udagawae
is able to grow at 10°C but fails to grow at >42°C. Furthermore, compared to
A. fumigatus
, the conidia of
N. udagawae
require longer incubation periods to germinate at 37°C and are more susceptible to neutrophil attack as well as hydrogen peroxide;
N. udagawae
is also less virulent in gp91

phox

−
/
−
mice. These findings suggest that growth and susceptibility to the host response might account for the reduced virulence of
N. udagawae
and the subtle distinction in the progression of the disease caused by the two species.
A Genetically Engineered Waterfowl Influenza Virus with a Deletion in the Stalk of the Neuraminidase Has Increased Virulence for Chickens
Authors: Munier S. , Larcher T. , Cormier-Aline F. , Soubieux D. , Su B. , Guigand L. , Labrosse B. , Cherel Y. , Quéré P. , Marc D. , Naffakh N. 
Published: November 2009

ABSTRACTA deletion of about 20 amino acids in the stalk of the neuraminidase (NA) is frequently detected upon transmission of influenza A viruses from waterfowl to domestic poultry. Using reverse genetics, a recombinant virus derived from a wild duck influenza virus isolate, A/Mallard/Marquenterre/Z237/83 (MZ), and an NA stalk deletion variant (MZ-delNA) were produced. Compared to the wild type, the MZ-delNA virus showed a moderate growth advantage on avian cultured cells. In 4-week-old chickens inoculated intratracheally with the MZ-delNA virus, viral replication in the lungs, liver, and kidneys was enhanced and interstitial pneumonia lesions were more severe than with the wild-type virus. The MZ-delNA-inoculated chickens showed significantly increased levels of mRNAs encoding interleukin-6 (IL-6), transforming growth factor-β4 (TGF-β4), and CCL5 in the lungs and a higher frequency of apoptotic cells in the liver than did their MZ-inoculated counterparts. Molecular mechanisms possibly underlying the growth advantage of the MZ-delNA virus were explored. The measured enzymatic activities toward a small substrate were similar for the wild-type and deleted NA, but the MZ-delNA virus eluted from chicken erythrocytes at reduced rates. Pseudoviral particles expressing the MZ hemagglutinin in combination with the MZ-NA or MZ-delNA protein were produced from avian cultured cells with similar efficiencies, suggesting that the deletion in the NA stalk does not enhance the release of progeny virions and probably affects an earlier step of the viral cycle. Overall, our data indicate that a shortened NA stalk is a strong determinant of adaptation and virulence of waterfowl influenza viruses in chickens.