bluuebunny/crossref_metadata_2025_split huggingface dataset:
135 Effects of altering diet acid-binding capacity-4 (ABC-4) with specialty soy protein sources or acidifiers on nursery pig performance and fecal dry matter
Authors: Stas Ethan , Tokach Mike D , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Gebhardt Jordan T 
Published: May 2024

Abstract
A total of 300 pigs (initial body weight = 6.0 kg) were used to evaluate the effects of altering diet acid-binding capacity-4 (ABC-4) with specialty soy protein sources or acidifiers on nursery pig performance and fecal dry matter (DM). At weaning, pigs were allotted to 1 of 5 dietary treatments. There were 5 pigs/pen and 12 replications/treatment. Pigs were fed experimental diets in two phases with phase 1 fed from d 0 to 10 post-weaning followed by phase 2 from d 10 to 24. A low ABC-4 diet was formulated to 200 and 250 meq/kg in phase 1 and 2, respectively. The low ABC-4 diet utilized 0.38% fumaric acid, 0.36% formic acid, and specialty soy protein concentrate (AX3 Digest; Protekta; Newport Beach, CA) at 9.38 and 7.50% of the diet in phase 1 and 2, respectively. For the first medium ABC-4 diet, specialty soy protein concentrate was replaced with enzymatically treated soybean meal (HP 300; Hamlet Protein; Findlay, OH) on an SID Lys-basis and resulted in an ABC-4 of 290 and 322 meq/kg for phase 1 and 2, respectively. For the second medium ABC-4 diet, acidifiers were removed resulting in an ABC-4 of 271 and 321 meq/kg for phase 1 and 2, respectively. In the high ABC-4 diet, both soy protein replacement and acidifier removal strategies were utilized resulting in ABC-4 values of 362 and 394 meq/kg for phase 1 and 2, respectively. In addition, a control diet was utilized with the same formulation as the high ABC-4 diet but with 3,000 and 2,000 mg/kg of Zn from ZnO in phase 1 and 2, respectively. Following phase 2, all pigs were fed a common diet until d 38 of the study. Increasing ABC-4 decreased (linear, P ≤ 0.043) gain to feed ratio (G:F) during the experimental period and overall (Table). Increasing ABC-4 decreased (linear, P ≤ 0.005) fecal DM on d 10 and 24, with no differences on d 17. Pigs fed the medium ABC-4 diet based on specialty soy protein replacement had increased (P = 0.003) fecal DM on d 10 compared with the medium ABC-4 diet where acidifiers were removed. Pigs fed diets containing pharmacological levels of Zn from ZnO had increased (P ≤ 0.047) body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and G:F during the experimental period but decreased (P = 0.005) ADG and G:F during the common period, with no differences overall compared with pigs fed diets not containing ZnO. In conclusion, as dietary ABC-4 increased from 200 to 362 meq/kg in phase 1 and 250 to 294 meq/kg in phase 2, growth performance and fecal DM decreased. This study suggests a dietary ABC-4 of 200 and 250 meq/kg in phase 1 and 2, respectively, can be utilized to improve growth performance and fecal dry matter of weanling pigs.
139 Effect of dietary crude protein concentration and sensory feed additive on nursery pig growth performance
Authors: Pound Kara , Richert Brian T , Scaff Taw , Fisk Alexandra , Cecil Mekenzie R , Otto-Tice Emily , Schinckel Allan P 
Published: May 2024

Abstract
Maximizing growth performance and feed intake for nursery pig diets is an ongoing challenge in the US swine industry. Using sensory feed additives is one strategy to optimize feed intake and growth rates of pigs. Weanling pigs (n = 416; average age = 19.8 d) were used to test the effects of sensory feed additive (SA; Luctamax Piglet, Lucta, Mahwah, NJ) addition to control (CON) and low crude protein (LCP; decreased 4% CP) nursery diets in a 2 x 2 factorial design (CON, CON+SA, LCP, LCP+SA). Decreased dietary CP was achieved by removing or decreasing fish meal and soy concentrate in phases 1 and 2 and reducing soybean meal in phase 3 and adding synthetic amino acids to maintain amino acid ratios (NRC 2012). Nursery treatments were fed in three phases; phase 1 (PH1, d 0-7, 0.10% SA), phase 2 (PH2, d 7-21, 0.10% SA), and phase 3 (PH3, d 21-36, 0.05% SA). Pigs were blocked by initial body weight (BW), sex, ancestry, and housed at 6 or 7 pigs/pen with 15 pens/treatment. Weekly pig BW and feed intake were used to calculate pen average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F). Data were analyzed using GLM procedure of SAS 9.4. During PH1 pigs receiving the CON diets tended (P = 0.065) to have a greater G:F compared with pigs receiving LCP diets. For the remainder of this study there were consistent interactions between dietary CP and SA. When SA was added to the LCP diet ADFI increased but when SA was added to CON diet ADFI decreased and these changes in feed intake often led to similar dietary interactions in ADG. During PH2, pigs fed CON or LCP+SA diets had greater ADFI (P = 0.032) and tended to have a greater ADG (P = 0.094) but reduced G:F (P = 0.100) compared with pigs fed CON+SA or LCP diets. In PH3, pigs fed CON or LCP+SA diets had a greater ADG (P = 0.027) and ADFI (P = 0.011) compared with CON+SA or LCP diets. Pigs fed LCP diets had greater ADFI (P = 0.001) but reduced G:F (P = 0.0001) than pigs fed CON diets. Overall, pigs fed CON or LCP+SA diets had greater ADFI (P = 0.016) and tended to have a greater ADG (P = 0.059) but reduced G:F (P = 0.067) compared with pigs fed CON+SA or LCP diets. Pigs fed CON diets had a greater G:F (P &lt; 0.001) compared with pigs fed LCP diets, while LCP pigs tended to have a greater ADFI (P = 0.054) compared with CON pigs. Inclusion of SA to LCP diet mitigated the adverse impact of replacing whole dietary proteins with high inclusion of synthetic amino acids. Careful consideration is needed when using sensory feed additives so as not to impede growth performance in diets containing traditionally greater levels of crude protein.
142 Evaluation of low SID lysine diets fed to enteric health challenged pigs on wean-to-finish performance
Authors: Miller Kayla A , Mendoza Omarh F , Shull Caleb M , Burrough Eric R , Gabler Nicholas K 
Published: May 2024

Abstract
Producers have been adopting low crude protein or low SID Lys nursery diets to address enteric health concerns. Therefore, we evaluated the longitudinal impact of these strategies on lifetime pig performance. Weaned mixed sex pigs n = 588; initial body weight (BW) = 6.81 ± 1.34 kg] sourced from a poor enteric health flow, were randomly assigned to pens and to one of four nursery dietary treatments, fed for 7 wk. Following the nursery period, pens were then assigned to one of two grow-finish treatments, fed until marketing, to complete a 2 × 2 × 2 factorial design (n = 6 pens/treatment, 12 to 13 pigs/pen). Phase 1 nursery diets were formulated to a 1.10% or 1.40% SID Lys (nLLys or nHLys) with amino acids (AA) provided by both soybean meal (SBM) and crystalline AA or SBM only (nLSBM or nHSBM, respectively). Nursery phase 2 and 3 diets were formulated with a 0.05% decrease in SID Lys while maintaining a 0.30% differential between high and low lysine treatments. Following the nursery, grow-finish pigs were fed diets formulated to equal SID Lys:ME with either a conventional crystalline AA (gfLSBM) or high SBM diet lacking crystalline AA (gfHSBM) to meet AA requirements. During the nursery, enteric health was assessed by fecal pathogen PCRs and bacterial culture, confirming a high natural rotavirus and F18 enterotoxigenic Escherichia coli burden. Pig BW and pen feed disappearance were recorded at the beginning and end of each phase to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (gain to feed ratio; G:F). Data were analyzed with pen as the experimental unit and the fixed effects of nursery SID Lys (nLys), nursery SBM (nSBM), grow-finish SBM (gfSBM), and the two- and three-way interactions using ANOVA. There were no three-way interactions for ADG or ADFI on final performance and no two-way interactions of nLys by nSBM in the nursery. Additionally, there was no effect of nSBM on overall nursery ADG, ADFI, and G:F (P &gt; 0.05). Pigs fed nHLys-gfHSBM (0.84 kg/d) had improved overall ADG versus pigs fed nLLys-gfHSBM (0.80 kg/d; interaction; P = 0.010). Final nursery ADG (0.47 vs. 0.42 kg/d), G:F (0.70 vs. 0.63), and ending BW (30.9 vs. 28.0 kg) were greater in pens fed nHLys compared with nLLys (P &lt; 0.001). Grow-finish pigs fed gfHSBM were more efficient compared with gfLSBM overall (0.457 vs. 0.449, respectively; P = 0.016). Although pigs fed nHLys had improved ending ADG compared with nLLys (0.83 vs. 0.81 kg/d; P = 0.018, respectively), there were no differences in final BW (128.0 vs. 127.0 kg) or ADFI (1.83 vs. 1.80 kg/d; P &gt; 0.05). In conclusion, low SID Lys enteric diets did not improve wean-to-market pig performance in enterically challenged pigs and there was no difference between amino acid sources (crystalline vs SBM).
144 Bioavailability of calcium salt of hydroxy analogue of methionine relative to DL-methionine to support growth performance of 11 to 20 kg pigs
Authors: Jayaraman Balachandar , Morales Joaquin , Rodriguez Maria , Htoo John K 
Published: May 2024

Abstract
Methionine (Met) is typically the third limiting AA in commercial swine diets and is commonly supplied as dry DL-Met (99% pure) or DL-Met hydroxy analogue. Data on the relative bioavailability (RBV) of calcium salt of hydroxy analogue of DL-Met (MHA-Ca; 84%) compared with DL-Met in weaned pigs is scarce. The objective of the experiment was to determine the RBV of MHA-Ca compared with DL-Met to support growth performance of 11 to 20 kg pigs. A total of 504 piglets [DNA x Pietrain pigs; approximately 42 d of age and 11 kg body weight (BW)] were used in two blocks of 252 pigs each with 6 pigs (3 entire males and 3 gilts) per pen and 12 replicate pens per treatment. Within each block, pigs were randomly allotted to 7 diets for 21 d. A basal diet (BD) was formulated to be adequate in all essential nutrients except for Met (0.22% standardized ileal digestible Met), which was 61% of the SID Met requirement of 0.36% recommended by NRC (2012) for 10 to 25 kg pigs. The Met-deficient BD was then supplemented with 3 graded levels of DL-Met (0.05, 0.10, and 0.15%) or MHA-Ca (0.077, 0.154, and 0.231%) at the expense of corn. Pigs had ad libitum access to feed (mash) and water. Individual pigs and pen feed disappearance were weighed on d 0 and d 21 to calculate average daily gain (ADG), average daily feed intake (ADFI) and feed conversion rate (FCR). On d 21, blood samples were collected from 1 pig per pen after 8 h fasting to determine total protein, albumin and glucose. Data were analyzed as a randomized complete block design using GLM procedure of SAS 9.4 (SAS Inst. Inc.). Orthogonal-polynomial contrasts were used to determine linear and quadratic effects of increasing levels of DL-Met and MHA-Ca, and the effect of Met sources. The final BW at d 21 and overall average daily gain (ADG; 375, 401, 429, 430, 402, 431, and 428 g/d for diets 1 to 7, respectively) increased linearly (P &lt; 0.01) by graded additions with both Met sources. The ADFI was not different but the overall FCR (1.64, 1.55, 1.51, 1.45, 1.59, 1.46, and 1.50 for diets 1 to 7, respectively) improved linearly (P &lt; 0.01) by supplementation with DL-Met or MHA-Ca. Pig performance responses (ADG, ADFI and FCR) was not different (P ≥ 0.15) when Met was supplemented in the Met-deficient diet at a DL-Met to MHA-Ca ratio of 65:100 on a product basis. Plasma concentrations of total protein, albumin and glucose on d 21 were not affected (P &gt;0.05) by dietary treatments or Met sources. Based on ADG as a response of Met supplementation level, the linear and exponential regressions estimated the RBV of 64 and 65%, respectively for MHA-Ca relative to DL-Met (product-to-product basis) in 11 to 20 kg pigs.
143 Effect of increasing dietary net energy content at a constant lysine on feed intake and performance of 7 to 11 kg and 11 to 20 kg pigs
Authors: Htoo John K , Mendoza S M S , Morales Joaquin 
Published: May 2024

Abstract
Information about NE requirements for nursery pigs is still limited. Therefore, 2 experiments were conducted to evaluate the effect of dietary NE contents on growth performance of 7 to 11 kg pigs (Exp. 1) and 11 to 20 kg pigs (Exp. 2). In Exp. 1, mixed-sex pigs (n = 192; PIC; initial body weight (BW) of 7.8 ± 1.07 kg] were assigned to 4 diets for 14 d. Diets 1 to 4 were formulated to contain 4 levels of NE (9.8, 10.1, 10.4 and 10.7 MJ/kg) and adequate in all AA with standardized ileal digestible (SID) Lys being constant at 1.42%. In Exp. 2, mixed-sex pigs (n = 192; PIC; initial BW of 11.2 ± 1.54 kg) were assigned to 4 diets for 21 d. Diets 1 to 4 contained 4 levels of NE (9.6, 9.9, 10.2 and 10.5 MJ/kg) and adequate in all AA (1.32% SID Lys). For both studies, there were 6 pen replicates (4 entire males and 4 gilts/pen) per treatment, and diets were based on corn, soybean meal, whey powder, wheat bran and soy oil. Pigs had ad libitum access to feed (mash) and water. Individual pigs and pen feed disappearance were weighed weekly to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency gain to feed ratio (G:F). Data were analyzed by ANOVA using the GLM procedure of SAS with pen as the experimental unit. In Exp. 1, ADFI tended to decrease (quadratic; P ≤ 0.08) with increasing NE levels during d 0 to 7 and d 8 to 14. The ADG during d 0 to 7 tended to be greater (158, 192, 187 and 152 g for diets 1 to 4; quadratic; P = 0.08) at 10.1 MJ/kg NE; however, the overall ADG (d 0 to 14) was not affected by the NE levels. Although not significant, G:F, Lys utilization efficiency were greatest at 10.1 MJ/kg NE for the overall period. In Exp. 2, ADFI decreased (827, 783, 768 and 749 g for diets 1 to 4; linear; P = 0.05) with increasing NE levels during d 15 to 21 but was not affected (P &gt; 0.05) for other periods. The ADG (d 8 to 14) tended to be greater at 9.6 MJ/kg NE (quadratic; P = 0.06) and G:F (d 15 to 21) tended to be greater at a dietary NE of 10.2 MJ/kg (linear; P = 0.10). However, the overall (d 0 to 21) ADG and G:F were not affected (P &gt; 0.05) by NE levels. Although not significant, G:F and Lys utilization efficiency were greatest at a dietary NE of 10.2 MJ/kg for the overall period. These results indicate that G:F of 7 to 11 kg pigs and 11 to 20 kg pigs maximized at a dietary NE content of 10.1 MJ/kg and 10.2 MJ/kg, which correspond to 1.41 and 1.29 g SID Lys/MJ NE, respectively.
301 Maxwell Lecture: Bacillus probiotics in nursery pigs: The effects of dose on gut health and performance
Authors: Jang Young Dal 
Published: May 2024

Abstract
Weaning is one of the most stressful events in the life of a pig and critical for their health and development. To reduce the use of antibiotics and zinc oxide in nursery diets, many feed additives have been tested to demonstrate their efficacy in weaning pigs. Bacillus subtilis, a heat-resistant spore-forming bacteria, consumes oxygen in the digestive tract and can provide a favorable anaerobic environment for lactic acid-producing bacteria, resulting in their proliferation. It also produces several digestive enzymes, such as amylase, protease, and antioxidant enzymes. In addition, Bacillus species are known to alter the gut microbiome and increase the production of short-chain fatty acids in the gut. Therefore, Bacillus probiotics have been widely used in nursery diets to effectively improve growth performance and protect piglets from weaning stress and postweaning diarrhea. However, when the probiotics are supplemented in diets for weaning pigs, their efficacy is dependent on multiple factors, such as strains, the health condition of the animals, product composition, and the dose of the probiotics. There has been tremendous effort in establishing the optimal supplementation level of probiotics for nursery pigs to maximize the efficacy of their supplementation, but a high dose of probiotics could be detrimental to the gut health and microflora of nursery pigs, as it could increase the incidence of diarrhea and may have potential side effects in the immune system, antibiotic resistance, and the production of unfavorable metabolites in the gut. A study was conducted to demonstrate the effect of Bacillus probiotic supplementation for nursery pigs at the recommended and high doses (10 times greater bacterial counts than the recommended dose) compared with a control treatment with no probiotic supplementation. In the study, the Bacillus supplementation at the recommended dose did improve the early postweaning growth rate, increase the production of fecal short-chain fatty acids (acetate, propionate, and butyrate), and blood glucose concentrations, and alter the fecal microbiome. However, high-dosing probiotics in nursery diets did not show any beneficial effects that were shown in the pigs fed diets with Bacillus probiotics at the recommended dose. Bacillus-probiotic supplementation in nursery diets may enhance growth performance and benefit gut health, but a high dose of probiotics does not always lead to comparable positive effects for pigs. Therefore, it is critically important to test probiotics to optimize their efficacy and avoid unnecessary high-dosing in nursery diets, as it could be cost-ineffective and may not show benefits that can be observed with the recommended dose.
152 Reducing the bioavailability of a mix of mycotoxins in broiler chickens with a clay- and algae-based decontaminant
Authors: Gallissot Marie , Rutjens Sofie , Rodriguez Maria , Devreese Mathias 
Published: May 2024

Abstract
Poultry feed is often contaminated with several mycotoxins that can impair animal health and performance, even at levels below the official guidance. The incorporation of mycotoxin decontaminants in the feed can limit these adverse effects. It is necessary to test the efficacy of these supplementations in vivo, but this requires specific experimental setups. The objective of this study was to test, in a validated toxicokinetic model, the ability of an algae-clay-based decontaminant to reduce the exposure of broilers to mycotoxin polycontamination (aflatoxin B1 – AFB1, ochratoxin A – OTA and deoxynivalenol, DON). The study included 16 male broiler chickens (Ross 308) of approximately 1.6 kg body weight (BW). After an acclimatization period of 1 wk, the broiler chickens were fasted for 12 h and then administered a single oral bolus with either the mycotoxins (n = 8) or the mycotoxins in combination with the decontaminant (n = 8; 2.0 mg AFB1/kg BW, 0.5 mg DON/kg BW, 0.25 mg OTA/kg BW, 2.5 g decontaminant/kg feed), administered in a parallel study design. Blood samples of the 16 broiler chickens were taken at different time points between 0 h (before administration) and 24 h (post administration; p.a.). The analysis of deoxynivalenol-3-sulphate (DON-3S, DON main metabolite), OTA and AFB1 in plasma was performed using UHPLC-MS/MS. The following parameters were calculated: area under the curve from time zero to the last point above the LOQ (AUC0→t), maximal plasma concentration (Cmax) and time at maximal plasma concentration (Tmax). Results showed that, using the oral bolus model, the mycotoxin decontaminant significantly altered the AUC0- &gt;12h of DON-3S in broiler chicken (P = 0.012). The relative oral bioavailability [(average AUC0- &gt;t mycotoxin + decontaminant/ average AUC0- &gt;t mycotoxin) * 100] of DON-3S when administered with the decontaminant was 60.1%. For OTA, the decontaminant decreased the absorption of OTA as indicated by a significantly decreased AUC0- &gt;24h (P &lt; 0.001). Consequently, the relative oral bioavailability of OTA when administered with the decontaminant was lowered as well, namely 55.7%. For AFB1, the decontaminant affected the absorption of AFB1 with a significantly decreased AUC0- &gt;8h (P &lt; 0.001), Cmax (P &lt; 0.001) and a significantly delayed Tmax (P = 0.025). Consequently, the relative oral bioavailability of AFB1 when administered with the decontaminant was 35.9%. Therefore, it can be concluded that the tested algae-clay based mycotoxin decontaminant reduces the oral absorption of multiple mycotoxins orally administered to broiler chickens in a single oral bolus model. These results are consistent with results obtained with DON in different experimental models (in vitro dynamics and in vivo performance) and confirm the efficacy of the decontaminant tested to control this mycotoxin in poultry production.
146 Comparing feeding strategies during heat stress in finishing pigs using a stochastic swine model
Authors: Aldenhoven Noud , Jaworski Neil W , Gutierrez Nestor A , Ferguson Neil S 
Published: May 2024

Abstract
Nutritionists may feed standard diets or increase dietary net energy (NE) and/or standardized ileal digestible lysine (SIDLys) to counter reduced average daily feed intake (ADFI) due to heat stress in the late finishing phase. The objective of this work was to develop a set of statistical tools to analyze different grow-finish feeding strategies using the simulations from a proprietary stochastic swine model (Watson) when heat stress occurs during the last 2 wk of the finishing phase. Financial considerations were excluded from the study. Nutrient recommendations for maximum feed efficiency were derived from Watson and used to formulate a grower diet for 25 to 60 kg body weight (BW), and four finisher diets for 60 to 135 kg BW: a) thermoneutral (10 MJ NE/kg, 7.9 g/kg SIDLys), b) high AA (10 NE/kg, 9.2 g/kg SIDLys), c) high NE (10.5 MJ/kg NE, 7.9 g/kg SIDLys), and d) high NE and high AA (10.5 MJ NE/kg, 8.3 g/kg SIDLys). The grow-finish period was simulated by feeding the grower diet followed by the finisher diets in 5 different scenarios: 1) thermoneutral, diet a; 2) heat stress, diet a; 3) heat stress, diet a, and b during last 2 wk; 4) heat stress, diet a, and c during last 2 wk; 5) heat stress, diet a, and d during last 2 wk. Performance results were obtained by running each scenario 100 times, generating a new population per simulation. Results from scenarios 1) and 2 showed that heat stress in the last 2 wk reduced (P &lt; 0.05) ADFI and average daily gain of pigs fed diet a by 790 g/d and 310 g/d, respectively. This resulted in a decrease of 4.79 kg in final BW, an increase of 0.64% lean yield, and a decrease of 0.80 mm back-fat at slaughter (P &lt; 0.05). An increased dietary SIDLys (3), NE (4), or both (5) during heat stress did not affect BW compared with scenario 2 (P &gt; 0.05). An increased NE (4) or NE and SIDLys (5) did not affect lean yield and back fat compared with scenario 2 (P &gt; 0.1). But increased SIDLys during heat stress (3), increased lean yield (P = 0.04) and a trend in less back fat (P = 0.07) compared with scenario 2. In conclusion, pig performance was reduced with heat stress in the late finishing period. With the developed statistical tools, we determined from the simulations that none of the proposed strategies significantly increased final BW compared with the baseline (2). But feeding greater SIDLys will likely result in significantly greater lean yield and less back fat. When formulating diets with greater nutrient density attention to ingredient cost and pig prices should be considered.