bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional architecture of the forebrain cholinergic system in rodents
Authors: Zaborszky Laszlo , Varsanyi Peter , Alloway Kevin , Chavez Candice , Gielow Matthew , Gombkoto Peter , Kondo Hideki , Nadasdy Zoltan 
Published: June 2024

Abstract

The basal forebrain cholinergic system (BFCS) participates in functions that are global across the brain, such as sleep-wake cycles, but also participates in capacities that are more behaviorally and anatomically specific, including sensory perception. To better understand the underlying organization principles of the BFCS, more and higher quality anatomical data and analysis is needed. Here, we created a “virtual Basal Forebrain”, combining data from numerous rats with cortical retrograde tracer injections into a common 3D reference coordinate space and developed a “spatial density correlation” methodology to analyze patterns in BFCS cortical projection targets, revealing that the BFCS is organized into three principal networks: somatosensory-motor, auditory, and visual. Within each network, clusters of cholinergic cells with increasing complexity innervate cortical targets. These networks represent hierarchically organized building blocks that may enable the BFCS to coordinate spatially selective signaling, including parallel modulation of multiple functionally interconnected yet diverse groups of cortical areas.
Bioequivalence evaluation of dexamethasone palmitate injections by intravenous injection in healthy Chinese volunteers: A 2-period crossover trial
Authors: Wang Bingna , Huang Chen , Xu Lang , Fu Xihua , Su Jianfen 
Published: June 2024

Abstract

Dexamethasone palmitate injection is mainly used in the treatment of severe rheumatoid arthritis.This study aimed to compare the bioequivalence and safety profiles of two (1 mL,:4 mg) dexamethasone palmitate injections in the fasting arm for healthy Chinese volunteers. A random, open, single-dose, two-preparations, two-sequences, two-period crossover design was conducted. Twenty-four healthy volunteers participated in the study. Each participant received an intravenous injection of either the test (imitation) or reference (original) formulation of dexamethasone palmitate in the fasting arm during each study period, with a 7-day washout period between administrations. The results showed that, under fasting condition, the pharmacokinetic parameters of the test and reference formulations were within the acceptable limits for bioequivalence. The 90% confidence intervals for the ratios of Cmax and AUC0 − t and AUC0−∞ fell within the bioequivalence range of 80–125%, achieving 100% power. No serious adverse events were observed, and no participants withdrew from the study. All safety parameters remained within normal limits throughout the study. The imitation preparation of dexamethasone palmitate injections is bioequivalent to the original formulation when administered as a single intravenous injection under fasting condition to healthy Chinese volunteers. This finding supports the interchangeability of the imitation and original formulations, potentially offering a cost-effective alternative in clinical practice. This study was registered in the Chinese Clinical Trial Registry (registration number: CTR20211598; date of registration: July 13, 2021).
The correlation between Hepatic Controlled Attenuation Parameter (CAP) value and Insulin Resistance (IR) was stronger than that between Body Mass Index, Visceral Fat Area and IR
Authors: Li Zhouhuiling , Liu Renjiao , Gao Xinying , Hou Dangmin , Leng Mingxin , Zhang Yanju , Du Meiyang , Zhang Shi , Li Chunjun 
Published: June 2024

Abstract

Background
Hepatic controlled attenuation parameter (CAP) value is a novel marker for quantifying hepatic fat accumulation. Insulin resistance (IR) plays a major role in the pathogenesis and natural history of hepatic steatosis. This study aimed to investigate the possible relationship between CAP value and IR.
Methods
This study included a total of 420 patients with overweight or obesity who came to the obesity clinic at Tianjin Union Medical Center. Vibration-controlled transient elastography examination was conducted to detect CAP and liver stiffness measurement (LSM) values. Body composition, including visceral fat area (VFA), and body fat mass (BFM), was evaluated by the direct segmental multi-frequency bioelectrical impedance analysis (BIA). The associations between CAP value, body mass index (BMI), VFA, BFM and homeostasis model assessment of insulin resistance (HOMA-IR) were analyzed.
Results
CAP value was positively associated with HOMA-IR (r = 0.568, P < 0.001), the strength of which was much stronger than BMI, VFA, and BFM. In multivariate linear regression, CAP value and HOMA-IR showed a significant positive association (adjusted β = 0.015, 95% CI: 0.007–0.022, P < 0.001). Subgroup analysis suggested no significant interaction between CAP value and HOMA-IR across age, BMI, LSM, hypertension, and sex groups (all P for interaction > 0.05).
Conclusions
Hepatic CAP value is more remarkably than other obesity markers associated with HOMA-IR in individuals with overweight or obesity, regardless of age, BMI, LSM, hypertension, and sex.
The associated between ALDH2 rs671 polymorphism and clinical prognosis in alcohol- related hepatocellular carcinoma after curative resection
Authors: Ko Chih-Jan , Wang Wen-Lung , Lin Hung-Yu , Hsieh Pei-Min , Chen Szu-Ying , Lin Steven Yu , Chou Li-Wei , Chen Yaw-Sen , Huang Yu-Wei , Ho Wen-Chao , Lin Chih-Wen 
Published: June 2024

Abstract

Background
Whether ALDH2 rs671 polymorphism predicts clinical prognosis in alcoholic liver disease (ALD)-related hepatocellular carcinoma (HCC) after hepatectomy remains unclear.
Aims
This study aims to investigate the association between ALDH2 rs671 polymorphism and HCC recurrence and mortality in patients with ALD-related HCC who underwent hepatectomy. We also explored the risk factors for HCC recurrence and mortality in this population of patients.
Methods
This prospective cohort study enrolled 238 ALD-related HCC patients underwent hepatectomy from 2011 to 2022 at the E-Da Hospital, I-Shou University. Data analyses were finalized on October, 2023. Alcoholism was defined as consuming over 20 g of ethanol each day for at least 5 years. Patients with HBsAg-positive or/and HCV-positive status were excluded. ALDH2 rs671 polymorphism was analyzed. The endpoint was HCC recurrence and overall mortality.
Results
Of the 238 patients enrolled, 196 (82.4%) were men, and the mean (SD) age was 62.3 (10.2) years. HCC recurrence occurred in 70 patients, and 64 patients died. ALDH2 rs671 polymorphism was significantly associated with HCC recurrence and mortality. The 10-year cumulative HCC recurrence and mortality rates were significantly higher in patients with the ALDH2 rs671 genotype GA/AA relative to those with the ALDH2 rs671 genotype GG. In the Cox proportional analyses, the ALDH2 rs671 genotypes GA/AA (hazard ratio [HR]: 2.66, 95% confidence interval [CI]: 1.59–4.43, P < 0.001) and AST ≥ 40 IU/L (HR: 1.93, 95% CI: 1.18–3.17, P = 0.009) were significantly associated with increased HCC recurrence. Furthermore, the ALDH2 rs671 genotype GA/AA (HR: 2.02, 95% CI: 1.17–3.49, P = 0.012) and age ≥ 65 years (HR: 1.67, 95% CI: 1.01–2.78, P = 0.048) were significantly associated with increased mortality.
Conclusions
The ALDH2 rs671 genotype GA/AA is significantly associated with unfavorable clinical prognosis in ALD-related HCC after hepatectomy.
Interaction of PHGDH with IGF2BP1 facilitates m6A-dependent stabilization of TCF7L2 mRNA to confer multi-drug resistance in gastric cancer
Authors: Chen Shuyi , Liu Dan , Liu Yi , Long Zhaobo , Wu Ying , Zhong Yue , Zhao Jun , Wu Tong , He Wenfang , Cao Tianyu , Fan Daiming , Wu Kaichun , Li Tingyu , Lu Yuanyuan , Zhao Xiaodi 
Published: June 2024

Abstract

Background
Multi-drug resistance (MDR) remains a significant barrier to effective chemotherapy and results in poor prognosis of gastric cancer (GC) patients. Exploring the mechanism of MDR is of great significance for identifying biomarkers of resistance and developing new treatment strategies.
Methods
The combination of multi-omics and bio-informatics analysis with experimental validation was adopted to explore the mechanism of MDR in gastric cancer (GC). Multi-omics analysis includes transcriptome (RNA-Seq) and proteome (iTRAQ-MS and HLC-MS) analysis. Bio-informatics analysis includes a series of differential analysis, enrichment analysis, correlation analysis, survival analysis and molecular docking. Experimental validation includes quantitative real-time PCR (RT-qPCR), Western blot, immunofluorescence (IF), immunohistochemistry (IHC), multiplex immunohistochemistry (mIHC), CCK−8 assay, Clone formation experiment, Flow cytometry, Luciferase reporter assay, RNA stability assay, co-immunoprecipitation (Co-IP), RNA-binding protein immunoprecipitation (RIP), Chromatin immunoprecipitation (ChIP), RNA pull down assay and animal studies.
Results
In our study, we found that phosphoglycerate dehydrogenase (PHGDH), the key rate-limiting enzyme in the serine synthesis pathway, was significantly up-regulated in MDR GC cells. PHGDH, acting its non-canonical function, was found out to promote MDR by promoting the Wnt/β-catenin signaling pathway mediated by transcription factor 7 like 2 (TCF7L2), a pivotal transcription factor in the Wnt pathway. Specifically, PHGDH stabilized TCF7L2 mRNA by interacting with insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1), a key m6A reader. PHGDH/IGF2BP1 interaction was m6A-dependent and led to the enhanced TCF7L2 mRNA stability and thus its up-regulation. Moreover, TCF7L2 bound to the PHGDH promoter and regulated PHGDH expression, acting in a feedback way.
Conclusions
The PHGDH/IGF2BP1-TCF7L2 axis plays a vital role in the MDR of GC and correlates with poor prognosis.
MEN1-mediated CD44 alternative splicing depression is required for ferroptosis during lung cancer suppression
Authors: Bangming Jin , Pan Ting , Zhu Jiamei , Wang Ziming , Tang Dekun , Wang Xuyan , Yang Yunqiao , Teng Yin , Tian Qianting , Dan Guixue , Chen Chaochun , Wang Houmei , Zhang Tuo , Guo Bing , Chen Tengxiang 
Published: June 2024

Abstract

Ferroptosis is a characteristic form of cell death triggered by excessive iron-dependent reactive oxygen species (ROS) and plays an important role in suppressing tumor development. Although MEN1-regulated cell-cycle arrest, apoptosis, and cell senescence have been considered foremost barriers to lung tumorigenesis, its other functions, such as RNA splicing regulation, are also important for its tumor suppression activity. Here, MEN1 was found to facilitate lipid ROS generation and sensitizes lung cancer cells to ferroptosis by depressing alternative CD44 pre-mRNA splicing. CD44 variant isoforms are highly expressed and correlated with poor prognosis in human lung cancers. Loss of Men1 profoundly accelerates the progression of Kras-mutant driven lung adenocarcinoma (LUAD), which is associated with the accumulation of CD44 variant isoforms found in a KrasG12D;Men1-specific deficient mouse model. Mechanistically, MEN1 maintains a relatively slow RNA polymerase II (Pol II) elongation by controlling the release of Pol II-associated factor 1 (PAF1) from the CD44 gene body, which in turn prevents the inclusion of CD44 variable exon (CD44v). Furthermore, CD44v6-interfering peptides effectively abrogate the growth and metastasis of established Kras-mutant LUAD and MEN1-deficient tumors by activating ferroptosis. The efficacy of CD44v6 peptides was stronger than that of erastin, a ferroptosis inducer, in MEN1-deficient tumors. Collectively, our study unveils a novel way of tumor suppression based on MEN1 regulation of CD44 alternative splicing, ROS production and ferroptosis.
Qualitative Parameters and Deterioration Kinetics of Palm Oil, Shea Butter and Their Blend Use for Frying Cheese
Authors: Kolawole Muftaudeen Taoheed , Ibrahim Adio Tayo , Ogundolie Frank , Adams Moses Dele , Olajugbagbe Temidayo Emmanuel , Titilayo Gbadanmosi Idayat 
Published: June 2024

Abstract

We investigated changes in the quality of palm oil, shea butter and their blend as affected by repeated usage in deep frying of cheese were investigated. Palm oil, shea butter and their blend (1:1 w/w) were used in repeated deep frying of cheese at 150oC for 15 minutes, repeated eight times, repeated for six (6) consecutive days Changes in oil quality indices such as free fatty acid, peroxide value, p-anisidine value, color index, iodine value, acid value, saponification value, specific gravity and refractive index were determined. Kinetic rate and Arrhenius equation were used to determine deterioration rate and activation energy Data were analyzed using ANOVA and regression at α==0.05. The free fatty acid value of palm oil (6.09% − 8.03%), shea butter (2.12% − 2.91%) and blend (3.30% − 4.50%) increased significantly. Palm oil recorded a high value of peroxide (6.20 meqO2/kg), while the lowest value was recorded by shea butter (3.20 meqO2/kg), the p-anisidine value of palm oil was higher (91.77) after the sixth frying cycle than shea butter (11.00) and their blend (46.17). Slight decrease in iodine value was observed in the blend (4.89–3.82), while palm oil decreased sharply (4.73–0.90). A high smoke point was recorded for shea butter (222.7oC and 226.4 oC), palm oil (204.30C and 218.2oC) and the blend oil (212.2oC and 216.6°C) within the six frying cycles.
The Prognostic Correlation of Heart rate variability and body weight status in liver cancer patients
Authors: Huang Qingqing , Liang Xiping , Feng Changyan , Adhikari Vishnu Prasad , Quan Xi , Yu Huiqing 
Published: June 2024

Abstract

Background
Weight status play an important role in the evolution and prognosis of cancer patients; however alterations of autonomic nervous system (ANS) caused by cancer may be the associated symptoms in cancer-related weight change.
Objective
The aim of this study is to identify the influence of weight status on ANS and determine whether ANS can be used as an index for early detection and prediction of weight changes in liver cancer patients with HRV.
Methods
We conducted a retrospective analysis of clinical data for newly diagnosed liver cancer from 2017 to 2022 in China. The authors conducted clinical and ECG data of liver cancer with special emphasis on advances ECG and the BMI aspect.
Results
Both BMI and KPS were lower in the liver cancer group compared to benign tumor patients and healthy controls (p ≤ 0.001). Liver cancer patients were more likely to have a history of diabetes (p = 0.005) and hepatitis B virus infection (p ≤ 0.001) compared to non-liver cancer patients. Moreover, the HRV indices and variations remained low in liver cancer patients Additionally, the mean average levels of SDNN and VLF bands in liver cancer remained significantly low (p ≤ 0.005). The results reveal that a lower BMI, lower VLF index, together with history of HBV infection positively correlated in liver cancer patients. The study reveals that HRV indices is associated with BMI in liver cancer patients with lesser body weight. As predicted, the LF and LF/VLF scores had correlation with lower BMI in the liver cancer patients (p < 0.05).
Conclusion
In liver cancer patients, decreased HRV was associated with a lower BMI independent of other con-founders. The role of HRV as a potential index for predicting cancer-related weight loss and improving the survival time of liver cancer patients are promising but require further validation in future studies.
Study on the inflammatory microenvironment of Epstein‒Barr virus-infected Hodgkin's lymphoma
Authors: Li Hongyu , Li Bingyu , Li Xun 
Published: June 2024

Abstract

Background
Epstein-Barr virus (EBV) is common in patients with Hodgkin's lymphoma (HL). It can lead to a loss of immune function, greatly increasing the HL risk. Clinical characteristics of RLH (reactive lymphoid hyperplasia) group and HL group were analyzed, and differences in cell morphology and CD30 expression were detected.
Results
There were no differences in clinical features between the RLH and HL groups. In terms of immune cell activity, compared with the RLH group, the HL group exhibited decreased proportions of CD4 + T cells and NK cells in lymphocytes, while the proportions of CD8 + T cells and CD19 cells were increased. The average expression rate of PD-1 was 51.14%, which indicated high expression. The expression of immune response products were significantly increased, and the expression of RIP2 was significantly decreased. The expression rates of IL-1β, IFN-α, INF-γ, TNF-α, IL-10, IL-6, IL-18, IL-17A, IL-8, IL-12P70, MCP-1, IL-23 and IL-33 were all increased in the patient group. WP5095, hsa04061, R-HSA-6785807, M196, hsa05321, and GO: 0032675 were significantly enriched pathways.
Conclusions
The changes of immune factors and inflammatory factors may also provide ideas for continuing to explore the biological treatment of EB positive Hodgkin lymphoma.
The value of fluorescence imaging in the intraoperative localization of small neuroendocrine tumors of the pancreas
Authors: Xi Shihang , Jiang Bin , Zheng Xingyuan , han Meng , Wang Xu , Wang Guannan , Jiang Yaqi , Fang Xiaosan , Qian Daohai , Muhammad Danish Irshad , Wang Xiaoming 
Published: June 2024

Abstract

Objective
To explore the effectiveness and safety of fluorescence imaging technology in the intraoperative localization of small neuroendocrine tumors of the pancreas.
Methods
Indocyanine green fluorescence imaging technology was used in operation, and the specific process of indocyanine green administration was as follows: 25 mg of indocyanine green was dissolved in 10 ml of sterilized water for injection, and 1 ml of indocyanine green was injected rapidly through the peripheral vein during the laparoscopic exploration of the tumor, and the tumor was observed to show green fluorescence about 1 min later, and the tumor showed green fluorescence, which was regarded as the success of development, and laparoscopic pancreatic tumor local excision surgery was carried out under the guidance of the real-time fluorescence imaging, and postoperative monitoring of the amylase index of drainage fluid to observe whether there was a pancreatic fistula. Under the real-time guidance of fluorescence imaging, laparoscopic pancreatic tumor local resection was performed, and the amylase index of the drainage fluid was monitored after surgery to observe whether pancreatic fistula occurred or not and to record the tumor grade, surgical margins, and other pathological conditions.
RESULTS
The fluorescence imaging effect of the two patients was good, the lesion boundary was clear, and both of them completed the local resection of the tumor laparoscopically without pancreatic fistula, and the pathology suggested that the pancreatic neuroendocrine tumors were grade G1, and the margins of the surgery were negative.
CONCLUSION
Fluorescence imaging technology helps to localize small pancreatic neuroendocrine tumors intraoperatively with good safety.
The association between retinol metabolic activity and the risk of hepatocellular carcinoma: an analysis based on bulk RNA sequencing and mendelian randomization
Authors: Chen Fuqing , Cai Yifan , Chen Xiangmei , Zhou Jianyin 
Published: June 2024

Abstract

Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality worldwide, with a 5-year survival rate still below 20%. Alterations in the levels of metabolites involved in retinol metabolism (RM) have been observed during HCC progression. However, the precise mechanisms underlying the involvement of RM-related genes in HCC development remain elusive. In this study, we conducted comprehensive bulk RNA sequencing analysis using publicly available databases and identified significant enrichment of retinol metabolism pathways in HCC. Furthermore, through Mendelian randomization analysis, we elucidated the causal relationship between retinol metabolism and HCC development. Subsequently, employing clustering analysis based on RM-related genes and utilizing univariate Cox proportional hazards regression, we constructed a prognostic risk model for HCC patients. Finally, our investigation into RDH16—a pivotal gene implicated in RM disorders—unveiled its potential functional role. Collectively, these findings highlight the diagnostic and prognostic value of distinct features associated with retinol metabolism for identifying HCC patients who would benefit from timely treatment interventions and achieve optimal prognosis.
Inflammatory Indices for Overall Survival in Hemodialysis Patients: A multicenter cohort study
Authors: Chen Xinpan , Wang Gang , Yin Xiayan , Liu Wenhu , Li Dishan , Huang Hongdong 
Published: June 2024

Abstract

Objective
This study aimed to determine the best inflammation and nutrition-related indicator for overall survival prediction in patients undergoing hemodialysis from 138 dialysis centers of Beijing.
Methods
This study included six indicators, for which optimal thresholds were calculated using an outcome-driven method. Kaplan-Meier estimator was utilized to evaluate OS, while multivariate Cox proportional hazard models and restricted cubic splines were employed to assess the relationship between these indicators and OS. C-index, receiver operating characteristic curves and decision curve analysis were used to evaluate the predictive ability of the different indicators.
Results
All indicators except for platelet-to-lymphocyte ratio were identified as independent predictors of OS. Among these indicators, the prognostic nutritional index (PNI) consistently demonstrated superior discriminatory ability in predicting outcomes among hemodialysis patients. The optimal cut-off value for PNI was determined to be 42.3. Each one-unit increase or standard deviation increase in PNI is associated with a 0.956-fold or 0.78-fold decrease in mortality risk.
Conclusions
PNI has demonstrated superior reliability as a prognostic indicator for hemodialysis patients compared to LCR, CAR, SII, PLR and NLR. The efficient assessment of PNI effectively identifies high-risk individuals and highlights its significance as a valuable prognostic tool in clinical settings.
Joint Optimization of Data Routing and Energy Cooperation for Multicastive WPT assisted Data Collection in WSNs
Authors: Jiang Yang , Gao Zhenguo , Wang Yakai , Amar Kaswan 
Published: June 2024

Abstract

In Energy Harvesting assisted Wireless Sensor Networks (EH-WSNs), Wireless Power Transfer (WPT) technology plays a vital role in extending network lifespan by enabling energy cooperation among nodes. Nevertheless, existing energy cooperation assisted data collection methods mostly focused solely on Point-to-Point (P2P) style WPT, overlooking the broadcasting nature of wireless radio signals, which offers the potential for multiple nodes to harvest energy simultaneously from a single radio signal. To best exploit the multicastive radio signals, this paper concentrates on harnessing multicastive WPT-based energy cooperation for optimizing data collection performance in WSNs, and inspects the underlying joint optimization of Data Routing and Energy Cooperation for Data Collection (DREC-DC) problem. Here the term \textit{multicastive WPT} is used to distinguish from the P2P-style WPT considered in previous works. To overcome the scalability limitations of centralized solutions, we proposed an efficient Distributed method based on Projected Subgradient Algorithm (DPSA) by formulizing and transforming the single-slot version DREC-DC problem into a strict convex form. For situations where relevant information in some upcoming time slots are available ahead through predictions, DPSA is extended to a sliding window style multi-slot version. Our DPSA is compared with a P2P style energy cooperation approach and some classic routing algorithms not involving energy cooperation for assessing its effectiveness. Simulation results demonstrate that our distributive multicastive-WPT based method leads to considerably higher data collection ability than others.