bluuebunny/crossref_metadata_2025_split huggingface dataset:
Avidity confers FcγR binding and immune effector function to aglycosylated immunoglobulin G1
Authors: Nesspor Thomas C. , Raju T. Shantha , Chin Chen ‐Ni , Vafa Omid , Brezski Randall J. 
Published: March 2012

ABSTRACTImmunoglobulin G (IgG) antibodies are an integral part of the adaptive immune response that provide a direct link between humoral and cellular components of the immune system. Insights into relationships between the structure and function of human IgGs have prompted molecular engineering efforts to enhance or eliminate specific properties, such as Fc‐mediated immune effector functions. Human IgGs have an N‐glycosylation site at Asn297, located in the second heavy chain constant region (CH2). The composition of the Fc glycan can have substantial impacts on Fc gamma receptor(FcγR) binding. The removal of the glycan through enzymatic deglycosylation or mutagenesis of the N‐linked glycosylation site has been reported to “silence” FcγR‐binding and effector functions, particularly with assays that measure monomeric binding. However, interactions between IgGs and FcγRs are not limited to monomeric interactions but can be influenced by avidity, which takes into account the sum of multimeric interactions between antigen‐engaged IgGs and FcγRs. We show here that under in vitro conditions, which allowed avidity binding, aglycosylated IgGs can bind to one of the FcγRs, FcγRI, and mediate effector functions. These studies highlight how the valency of a molecular interaction (monomeric binding versus avidity binding) can influence antibody/FcγR interactions such that avidity effects can translate very low intrinsic affinities into significant functional outcomes. Copyright © 2012 John Wiley & Sons, Ltd.
Drivers of freelance career success
Authors: van den Born Arjan , van Witteloostuijn Arjen 
Published: March 2012

SummaryRecent evidence shows that the frequently proclaimed collapse of the traditional career model is actually not supported by job tenure data. This paper argues that the observed stability of job tenure might be explained by an increasing number of shamrock organizations. This organizational form has three types of workers: core employees, professional freelancers, and routine workers. In such an organization, two very different career models coexist. The organization largely determines the career of the core employee, whereas the individual essentially shapes that of the professional freelancer. This paper studies extensively the career of this second group: the professional freelancer, a growing phenomenon in many developed countries but not yet the focus of many career studies. We develop a freelance career success model on basis of the intelligent career framework augmented by insights from literature on entrepreneurship. Data are from a web survey with responses from about 1600 independent professionals in the Netherlands, in combination with 51 in‐depth interviews. We provide two main contributions. First, we report findings from the first large‐scale quantitative study into freelance career success. Second, this study enhances our understanding of the success of the modern career by building bridges between career and entrepreneurship literatures. We conclude that the external environment in which an individual freelancer operates is the most important factor determining career success. The study therefore suggests that more work needs to be performed on the relationship between the environment and individual career success. Copyright © 2012 John Wiley & Sons, Ltd.
Creep behaviour of a casting titanium carbide reinforced AlSi12CuNiMg piston alloy at elevated temperatures
Authors: Zak H. , Michel S. , Tonn B. , Scholz A. 
Published: March 2012

AbstractIm Rahmen dieser Arbeit wird das Kriechverhalten der dispersionsverstärkten Legierung AlSi12CuNiMg bei 350°C untersucht und mit der konventionellen Kolbenlegierung AlSi12Cu4Ni2MgTiZr verglichen. Die dispersionsverstärkte Legierung zeichnet sich gegenüber der Legierung AlSi12Cu4Ni2MgTiZr durch einen geringeren Kupfer‐ und Nickelgehalt aus und wird in einem kostengünstigen schmelzmetallurgischen Verfahren durch Zugabe der TiC‐haltigen Vorlegierung zur Aluminiumschmelze hergestellt. Es zeigt sich, dass die neu entwickelte Kolbenlegierung über die gleiche Kriech‐ und Zeitstandfestigkeit wie die konventionelle Legierung AlSi12Cu4Ni2MgTiZr verfügt.
Atypical venous glomangioma causing chronic compression of the radial sensory nerve in the forearm. A case report and review of the literature
Authors: Jiga Lucian P. , Rata Andreea , Ignatiadis Ioannis , Geishauser Max , Ionac Mihai 
Published: March 2012

AbstractExtrinsic chronic nerve compression induced by nonendothelium derived vascular tumors is a rare occurrence at the forearm level. We present a case of severe chronic compression of the radial sensory nerve (RSN) caused by an undiagnosed venous glomangioma. The tumor was excised with complete symptoms relief. In the presence of severe nerve compression syndromes in young age, without predisposing comorbidities, atypical extrinsic compression due to vascular tumors should be considered. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
A shape‐free 8‐node plane element unsymmetric analytical trial function method
Authors: Cen Song , Zhou Guo‐Hua , Fu Xiang‐Rong 
Published: March 2012

SUMMARYThe unsymmetric FEM is one of the effective techniques for developing finite element models immune to various mesh distortions. However, because of the inherent limitation of the metric shape functions, the resulting element models exhibit rotational frame dependence and interpolation failure under certain conditions. In this paper, by introducing the analytical trial function method used in the hybrid stress‐function element method, an effort was made to naturally eliminate these defects and improve accuracy. The key point of the new strategy is that the monomial terms (the trial functions) in the assumed metric displacement fields are replaced by the fundamental analytical solutions of plane problems. Furthermore, some rational conditions are imposed on the trial functions so that the assumed displacement fields possess fourth‐order completeness in Cartesian coordinates. The resulting element model, denoted by US‐ATFQ8, can still work well when interpolation failure modes for original unsymmetric element occur, and provide the invariance for the coordinate rotation. Numerical results show that the exact solutions for constant strain/stress, pure bending and linear bending problems can be obtained by the new element US‐ATFQ8 using arbitrary severely distorted meshes, and produce more accurate results for other more complicated problems. Copyright © 2012 John Wiley & Sons, Ltd.
CD133 marks a myogenically primitive subpopulation in rhabdomyosarcoma cell lines that are relatively chemoresistant but sensitive to mutant HSV
Authors: Pressey Joseph G. , Haas Marilyn C. , Pressey Christine S. , Kelly Virginia M. , Parker Jacqueline N. , Gillespie G. Yancey , Friedman Gregory K. 
Published: March 2012

AbstractBackgroundRhabdomyosarcoma (RMS) is characterized by features of skeletal muscle and is comprised of two major histological subtypes, embryonal (E‐RMS), and alveolar (A‐RMS). Subsets of each RMS subtype demonstrate resistance to multimodal therapy leading to treatment failure. Cancer stem cells or cancer‐initiating cells (CIC) represent a theorized population of cells that give rise to tumors and are responsible for treatment resistance.ProcedureWe investigated the ability of CD133, a putative CIC marker, to distinguish a chemoresistant, myogenically primitive population in alveolar (RH30), and embryonal (RD) RMS cell lines. We tested CD133+/− cells for sensitivity to engineered herpes simplex virus (oHSV).ResultsRelative to CD133− cells, CD133+ A‐RMS, and E‐RMS cells demonstrate an enhanced colony‐forming ability, are less differentiated myogenically, and are more resistant to cytotoxic chemotherapy but equally sensitive to oHSV oncolysis. Compared to CD133− RD cells, CD133+ cells express relatively high levels of genes typically expressed in skeletal muscle progenitor satellite cells including PAX7, c‐MET, and the GLI effectors of the hedgehog signaling pathway. In contrast, CD133+ RH30 cells were not associated with enhanced expression of satellite cell markers or Hh targets.ConclusionsOur findings demonstrate that CD133+ cells from A‐RMS and E‐RMS cell lines are characterized by a myogenically primitive phenotype. These cells have the capacity to form colonies in vitro and are more resistant to chemotherapy than CD133− cells. CD133 expression may denote a subset of RMS cells with an important role in tumorigenesis and treatment failure. These resistant cells may be effectively targeted by oHSV therapy. Pediatr Blood Cancer 2013; 60: 45–52. © 2012 Wiley Periodicals, Inc.
Imaging in gynecological disease (7): clinical and ultrasound features of Brenner tumors of the ovary
Authors: Dierickx I. , Valentin L. , Van Holsbeke C. , Jacomen G. , Lissoni A. A. , Licameli A. , Testa A. , Bourne T. , Timmerman D. 
Published: March 2012

ABSTRACTObjectivesTo describe clinical and ultrasound features of Brenner tumors of the ovary.MethodsIn this retrospective study, the databases of the International Ovarian Tumor Analysis (IOTA) studies and one tertiary center were searched to identify patients who had undergone an ultrasound scan before surgery for an adnexal mass that proved to be a Brenner tumor. Twenty‐eight patients with 29 Brenner tumors were included, most of which had been collected within the framework of the IOTA studies. An experienced ultrasound examiner reviewed available ultrasound images (available for 14 tumors), searching for a pattern specific to Brenner tumors.ResultsMost patients were postmenopausal and asymptomatic. Twenty‐four (83%) tumors were benign, two (7%) were borderline and three (10%) were malignant. Most benign tumors (17/24, 71%) contained solid components and manifested no or minimal blood flow on Doppler examination (19/24, 79%). Information about calcifications was available for 15 benign tumors, and in 13 (87%) calcifications were present. The five borderline and invasively malignant tumors contained solid components less often than did the benign ones (3/5, 60%) and were more richly vascularized on Doppler examination. Information about calcifications was available for four borderline or invasively malignant tumors, and in three (75%) calcifications were present.ConclusionWe failed to demonstrate ultrasound features specific to Brenner tumors. A prospective study is needed to determine if ultrasound features of calcifications can discriminate between Brenner tumors and other types of ovarian tumor. Copyright © 2012 ISUOG. Published by John Wiley & Sons, Ltd.
Integration of proteomics into systems biology of cancer
Authors: Hanash S. , Schliekelman M. , Zhang Q. , Taguchi A. 
Published: March 2012

AbstractDeciphering the complexity and heterogeneity of cancer, benefits from integration of proteomic level data into systems biology efforts. The opportunities available as a result of advances in proteomic technologies, the successes to date, and the challenges involved in integrating diverse datasets are addressed in this review. WIREs Syst Biol Med 2012 doi: 10.1002/wsbm.1169This article is categorized under:

Translational, Genomic, and Systems Medicine > Diagnostic Methods
Laboratory Methods and Technologies > Proteomics Methods
Translational, Genomic, and Systems Medicine > Translational Medicine
Cassette series designed for live‐cell imaging of proteins and high‐resolution techniques in yeast
Authors: Young Carissa L. , Raden David L. , Caplan Jeffrey L. , Czymmek Kirk J. , Robinson Anne S. 
Published: March 2012

AbstractDuring the past decade, it has become clear that protein function and regulation are highly dependent upon intracellular localization. Although fluorescent protein variants are ubiquitously used to monitor protein dynamics, localization and abundance; fluorescent light microscopy techniques often lack the resolution to explore protein heterogeneity and cellular ultrastructure. Several approaches have been developed to identify, characterize and monitor the spatial localization of proteins and complexes at the suborganelle level, yet many of these techniques have not been applied to yeast. Thus, we have constructed a series of cassettes containing codon‐optimized epitope tags, fluorescent protein variants that cover the full spectrum of visible light, a TetCys motif used for fluorescein arsenical hairpin (FlAsH)‐based localization, and the first evaluation in yeast of a photoswitchable variant, mEos2, to monitor discrete subpopulations of proteins via confocal microscopy. This series of modules, complete with six different selection markers, provides the optimal flexibility during live‐cell imaging and multicolour labelling in vivo. Furthermore, high‐resolution imaging techniques include the yeast‐enhanced TetCys motif, which is compatible with diaminobenzidine photo‐oxidation used for protein localization by electron microscopy, and mEos2, which is ideal for super‐resolution microscopy. We have examined the utility of our cassettes by analysing all probes fused to the C‐terminus of Sec61, a polytopic membrane protein of the endoplasmic reticulum of moderate protein concentration, in order to directly compare fluorescent probes, their utility and technical applications. Our series of cassettes expand the repertoire of molecular tools available to advance targeted spatiotemporal investigations using multiple live‐cell, super‐resolution or electron microscopy imaging techniques. Copyright © 2012 John Wiley & Sons, Ltd.
Incorporating Rare Earth Metal Complexes and Conjugated Organic Cations into Polymeric Iodoargentate: Structures and Properties of Two Hybrid Iodoargentates
Authors: Li Hao‐Hong , Huang Shu‐Wei , Lian Zhao‐Xun , Liu Ji‐Bo , Wang Min , Chen Zhi‐Rong 
Published: March 2012

AbstractTwo new polymeric iodoargentate hybrids induced by rare earth metal complexes and conjugated organic cations, {[Er(DMF)8][Ag6I9]}n (1) and [(MMP)(AgI2)]n (2) (MMP = 1‐methyl‐4‐methylpyridinium) were obtained by the routine synthetic reaction and structurally determined. In compound 1, a (Ag6I9)n3n– chain is defined by edge‐sharing Ag6I12 units, and in 2, the (Ag2I4)n2– chains are constructed by edge‐sharing AgI4 tetrahedra. C–H···I hydrogen bonds between cations and iodoargentate polyanions contribute to their structural extension from 1D to a 2D layer (for 1) and a 3D network (for 2). The UV/Vis spectra indicate that compared with 2, compound 1 exhibits a narrower adsorption scope with an onset at about 360 nm, and it has a much wider bandgap of about 3.50 eV.
Pr5F[SiO4]2[SeO3]3: Another Complex Fluoride Oxosilicate Oxoselenate(IV)
Authors: Lipp Christian , Burns Peter C. , Schleid Thomas 
Published: March 2012

AbstractDuring attempts to synthesize lanthanoid(III) fluoride oxoselenates(IV) with the simple composition MF[SeO3], not only Pr3F[SeO3]4, but also Pr5F[SiO4]2[SeO3]3 appeared as pale green crystalline by‐products in the case of praseodymium. Pr5F[SiO4]2[SeO3]3 crystallizes triclinically in space group P$\bar{1}$ (no. 2) with a = 701.14(5), b = 982.68(7), c = 1286.79(9) pm, α = 70.552(3), β = 76.904(3), γ = 69.417(3)° and Z = 2. The five crystallographically different Pr3+ cations on the general positions 2i show coordination numbers of eight and nine. [(Pr1)O8]13– and [(Pr2)O8]13– polyhedra are connected to$\bar{1}${[(Pr1, 2)2O12]18–} chains along the [100] direction. [(Pr3)O7F]12–, [(Pr4)O8F]14– and [(Pr4)O8F]14– polyhedra generate [F(Pr3, 4, 5)3O19]30– units about their central F– anion in triangular Pr3+ coordination. These units form $\bar{1}${[F(Pr3, 4, 5)3O16]24–} strands, again running parallel to [100]. Their alternating connection with the $\bar{1}${[(Pr1, 2)2O12]18–} chains results in $\bar{1}${[Pr5O20F]26–} sheets parallel to the (001) plane. Like in the already known related compound Er3F[SiO4][SeO3]2, a three‐dimensional network $\bar{1}${[Pr5O17F]20–} is achieved without the contribution of both the tetravalent silicon and selenium components. However, two Si4+ and three Se4+ cations forming tetrahedral [SiO4]4– and ψ1‐tetrahedral [SeO3]2– units with all O2– anions guarantee the charge balance. The formation of Pr5F[SiO4]2[SeO3]3 was observed when praseodymium sesquioxide (Pr2O3: in‐situ produced from Pr and Pr6O11 in a molar ratio of 3/11:4/11),praseodymium trifluoride (PrF3) and selenium dioxide (SeO2) in 1:1:3 molar ratios were reacted with CsBr as fluxing agent for five days at 750 °C in evacuated fused silica (SiO2) ampoules.
Synthesis, Structure, and Solution Study of a Mercury(II) Complex with the Ligand [1‐(2‐Methoxyphenyl)‐3‐(4‐chlorophenyl)]triazene
Authors: Rofouei Mohammad Kazem , Ghalami Zahra , Attar Gharamaleki Jafar , Ghoulipour Vanik , Bruno Giuseppe , Rudbari Hadi Amiri 
Published: March 2012

AbstractThe title ligand, [1‐(2‐methoxyphenyl)‐3‐(4‐chlorophenyl)]triazene, HL (1), was prepared. In a reaction with Hg(NO3)2 it forms the complex [Hg(C26H22Cl2N6O2)], [HgL2] (2). Both compounds were characterized by means of X‐ray crystallography, CHN analysis, FT‐IR, 1H NMR, and 13C NMR spectroscopy. In the structure of compound 1, two independent fragments are present in the unit cell. They exhibit trans arrangement about the –N=N– double bond. The dihedral angles between two benzene rings in both fragments are 4.36 and 18.79 Å, respectively. Non‐classic C–H···N hydrogen bonding and C–H···π interactions form a layer structure along the crystallographic ab plane [110]. In compound 2, the HgII atom is hexacoordinated by two tridentate [1‐(2‐methoxyphenyl)‐3‐(4‐chlorophenyl)]triazenide ligands through a N2O2 set. In addition, in the structure of 2, monomeric complexes are connected to each other by C–H···π stacking interactions, resulting in a 2D architecture. These C–H···π edge‐to‐face interactions are present with H···π distances of 3.156 and 3.027 Å. The results of studies of the stoichiometry and formation of complex 2 in methanol solution were found to support its solid state stoichiometry.
Synthesis and Reaction Chemistry of Sb(ECH2CH2NMe2)3 (E = O, S)
Authors: Horley Graeme A. , Kociok‐Köhn Gabriele , Molloy Kieran C. , Rodriguez Castro Jorge , Morreale Sergio 
Published: March 2012

AbstractThe antimony aminoalkoxide and aminothiolates Sb(ECH2CH2NMe2)3 [E = O (1), S (2)] were synthesized and their ability to form adducts with other metal moieties investigated. Compound 1 forms 1:1 adducts with NiI2 (3) and M(acac)2 [M = Cd (4), Ni (5)], while 2 undergoes ligand exchange with AlMe3 to afford Me2AlSCH2CH2NMe2 (6). The structures of 2–4 and 6 were determined. Compound 2 incorporates three S, N‐chelating ligands though the interaction with nitrogen is weaker than in analogous alkoxide complexes. Product 3 reveals one iodine has migrated from nickel to antimony, and all three alkoxide ligands bridge the two metals through μ2‐O atoms. In contrast, in 4, only one alkoxide links the antimony and cadmium. Compound 6 adopts the same structure, a chelating S,N ligand generating a tetrahedral center at aluminum, as known tBu2AlSCH2CH2NR2 species (R = Me, Et).
Lewis Acid Base Reactions between Boron Trifluoride and Complex Oxoanions as a Versatile Access to Fluorooxoanions: Synthesis of Sodium (Trifluoroborato)sulfate
Authors: Pilz Thomas , Jansen Martin 
Published: March 2012

AbstractNa2SO4BF3, synthesized in a closed vessel from BF3 and Na2SO4 at 603 K, crystallizes in the triclinic space group P$\bar{1}$ (a = 6.6033(2), b = 6.6328(2), c = 6.6349 (1) Å, α = 84.542(2), β = 84.458(1) and γ = 65.762(1)°). The novel fluorooxo anion [SO4BF3]2– displays a structure similar to the disulfate anion S2O72–. Heating the title compound in the absence of BF3 in the gas phase leads to decomposition even at 553 K, which is below the temperature of synthesis under BF3 pressure.
Challenges of Surgery in Developing Countries: A Survey of Surgical and Anesthesia Capacity in Uganda’s Public Hospitals
Authors: Linden Allison F. , Sekidde Francis Serufusa , Galukande Moses , Knowlton Lisa Marie , Chackungal Smita , McQueen K. A. Kelly 
Published: March 2012

AbstractBackgroundThere are large disparities in access to surgical services due to a multitude of factors, including insufficient health human resources, infrastructure, medicines, equipment, financing, logistics, and information reporting. This study aimed to assess these important factors in Uganda’s government hospitals as part of a larger study examining surgical and anesthesia capacity in low‐income countries in Africa.MethodsA standardized survey tool was administered via interviews with Ministry of Health officials and key health practitioners at 14 public government hospitals throughout the country. Descriptive statistics were used to analyze the data.ResultsThere were a total of 107 general surgeons, 97 specialty surgeons, 124 obstetricians/gynecologists (OB/GYNs), and 17 anesthesiologists in Uganda, for a rate of one surgeon per 100,000 people. There was 0.2 major operating theater per 100,000 people. Altogether, 53% of all operations were general surgery cases, and 44% were OB/GYN cases. In all, 73% of all operations were performed on an emergency basis. All hospitals reported unreliable supplies of water and electricity. Essential equipment was missing across all hospitals, with no pulse oximeters found at any facilities. A uniform reporting mechanism for outcomes did not exist.ConclusionsThere is a lack of vital human resources and infrastructure to provide adequate, safe surgery at many of the government hospitals in Uganda. A large number of surgical procedures are undertaken despite these austere conditions. Many areas that need policy development and international collaboration are evident. Surgical services need to become a greater priority in health care provision in Uganda as they could promise a significant reduction in morbidity and mortality.