bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does the amplatzer septal occluder device alter ventricular contraction pattern? A ventricular motion analysis by MR tagging
Authors: Stern Heiko , Baurecht Hansjörg , Luechinger Roger , Schwaiger Markus , Hess John , Fratz Sohrab 
Published: January 2012

AbstractPurpose:To assess by cardiovascular magnetic resonance (CMR) and CMR tagging if the Amplatzer Septal Occluder affects right ventricular (RV) and left ventricular (LV) motion pattern.Materials and Methods:Sixteen consecutive patients with significant atrial septal defect (ASD) and nine consecutive patients with persistent foramen ovale (PFO) as controls were studied before and a median of 14 days after defect closure by an Amplatzer occluder. By CMR end‐diastolic (EDV) and end‐systolic (ESV) RV and LV volumes were determined. Aortic and pulmonary artery flow was measured for assessment of left‐to‐right shunt (Qp/Qs). By CMR tagging circumferential strain and radial shortening, maximal rotation and torsion were measured,Results:In ASD patients RV‐EDV and RV‐ESV decreased (P < 0.05). LV‐EDV and LV‐ESV increased after ASD closure (P < 0.005). Qp/Qs dropped from 1.8 to 1.0 (P < 0.001). PFO patients showed no ventricular volume change after PFO closure. In ASD patients circumferential strain and radial shortening and maximal rotation of the RV decreased by ASD closure (P < 0.01). In LV only maximal rotation at the base and apex decreased significantly (P < 0.05). Torsion remained constant. In PFO patients no tagging parameter changed after defect closure.Conclusion:The Amplatzer occluder itself does not change the ventricular contraction pattern. All volume and myocardial deformation changes were caused by ventricular loading shifts. J. Magn. Reson. Imaging 2012;35:949–956. © 2012 Wiley Periodicals, Inc.
Comparison of visceral adipose tissue quantification on water suppressed and nonwater‐suppressed MRI at 3.0 tesla
Authors: Zhou Anqi , Murillo Horacio , Cusi Kenneth , Peng Qi 
Published: January 2012

AbstractPurpose:To systematically evaluate and compare the performance of water‐saturated and nonwater‐saturated T1‐weighted 3.0 T magnetic resonance imaging (MRI) in the application of visceral adipose tissue (VAT) quantification.Materials and Methods:Forty‐five patients underwent abdomen MRI using two different sequences at 3.0 T: 1) a traditional T1‐weighted gradient echo sequence, and 2) the same sequence with water presaturation to enhance fat and nonfat contrast. VAT amounts from both water‐saturated and nonwater‐saturated images were quantified with a manual thresholding technique and an automated segmentation method to study quantification variability and consistency of the two imaging techniques.Results:Nonwater‐saturated MRI had significantly larger coefficient of variation than water‐saturated MRI in the imaging reproducibility study based on 112 slices from seven subjects (11.4% vs. 2.5%, P < 0.0001). VAT volumes measured from the nonwater‐saturation MRI sequence had significantly higher variability than those from water‐saturation images even when using a manual quantification method based on images from 38 subjects (1.76% vs. 1.08%, P < 0.001). In addition, the VAT volume amounts from nonwater‐saturation images and water‐saturated images quantified with the automatic and manual quantification methods were statistically consistent.Conclusion:Water‐saturated MRI sequences at 3.0 T for VAT quantification improve reproducibility and decrease variability compared with nonwater saturated sequences, especially with the use of automatic quantification methods. J. Magn. Reson. Imaging 2012;35:1445–1452. © 2012 Wiley Periodicals, Inc.
Detection and localization of prostate cancer with the targeted biopsy strategy based on ADC Map: A prospective large‐scale cohort study
Authors: Watanabe Yuji , Terai Akito , Araki Tohru , Nagayama Masako , Okumura Akira , Amoh Yoshiki , Ishimori Takayoshi , Ishibashi Mana , Nakashita Satoru , Dodo Yoshihiro 
Published: January 2012

AbstractPurpose:To investigate the usefulness of targeted biopsy strategy based on apparent diffusion coefficient (ADC) maps in the detection and localization of prostate cancer.Materials and Methods:Institutional review board approval and informed consent from all participants were obtained. This study included 1448 consecutive patients suspected of having prostate cancer based on PSA level, who were divided into two groups: Group A included 890 patients with low‐ADC lesions who underwent targeted and systematic biopsies; Group B included 558 patients with no low‐ADC lesions who underwent only systematic biopsies. The cancer detection rates (CDR) of each group, positive predictive value (PPV), and negative predictive value (NPV) of ADC maps were calculated.Results:The CDR was 70.1% for Group A, higher than those for overall patients (48.1%) and for Group B (13.1%) with significant difference (P < 0.001). In the serum, PSA range from 4 to 20 ng/mL, the CDR was higher for the Group A than for the Group B and overall patients with significant differences. PPV and NPV of MR findings were 70.1% and 86.9%, respectively. Especially, the PPV of the MR findings for the anterior portion was as high as 90.1%. Among the false negatives of MR findings, Gleason score proved 6 or smaller in 79.5%, and positive core number was merely one or two in 80.8%.Conclusion:The targeted biopsy strategy based on ADC maps can be useful in the detection and localization of prostate cancer with high PPV. J. Magn. Reson. Imaging 2012;35:1414–1421. © 2012 Wiley Periodicals, Inc.
Osteogenic and chondrogenic potential of biomembrane cells from the PMMA‐segmental defect rat model
Authors: Gruber Helen E. , Riley Frank E. , Hoelscher Gretchen L. , Bayoumi Essraa M. , Ingram Jane A. , Ramp Warren K. , Bosse Michael J. , Kellam James F. 
Published: January 2012

AbstractA layer of cells (the “biomembrane”) has been identified in large segmental defects between bone and surgically placed methacrylate spacers or antibiotic‐impregnated cement beads. We hypothesize that this contains a pluripotent stem cell population with potential valuable applications in orthopedic tissue engineering. Objectives using biomembranes harvested from rat segmental defects were to: (1) Culture biomembrane cells in specialized media to direct progenitor cells along bone or cartilage cell differentiation lineages; (2) evaluate harvested biomembranes for mesenchymal stem cell markers, and (3) define relevant gene expression patterns in harvested biomembranes using microarray analysis. Culture in osteogenic media produced mineralized nodules; culture in chondrogenic media produced masses containing chondroitin sulfate/sulfated proteoglycans. Molecular analysis of biomembrane cells versus control periosteum showed significant upregulation of key genes functioning in mesenchymal stem cell differentiation, development, maintenance, and proliferation. Results identified significant upregulation of WNT receptor signaling pathway genes and significant upregulation of BMP signaling pathway genes. Findings confirm that the biomembrane has a pluripotent stem cell population. The ability to heal large bone defects is clinically challenging, and novel tissue engineering uses of the biomembrane hold great promise in treating non‐unions, open fractures with large bone loss and/or infections, and defects associated with tumor resection. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1198–1212, 2012
Effect of soy flour addition and heat‐processing method on nutritional quality and consumer acceptability of cassava complementary porridges
Authors: Muoki Penina N , de Kock Henriëtte L , Emmambux Mohammad Naushad 
Published: January 2012

AbstractBACKGROUND: The nutritional quality of cassava complementary porridge was improved through extrusion cooking and compositing with either defatted or full fat soy flour (65:35 w/w), and product acceptability by mothers with children of the target population was evaluated.RESULTS: The protein digestibility‐corrected amino acid score (PDCAAS) of extrusion‐ and conventionally cooked composite porridges was within the recommendations for complementary foods. The kinetics of starch digestibility showed that all porridges had a rapid rate of starch digestibility, but the rate was lower when defatted soy flour was added and lowest when full fat soy flour was added. The formation of amylase‐lipid complexes as shown by X‐ray diffraction and differential scanning calorimetry can be attributed to the lower digestibility of extrusion‐cooked porridge with full fat soy flour. If fed thrice per day, extrusion‐cooked porridge with defatted or full fat soy flour would meet the energy, protein and available lysine requirements of a child aged 6–8 months receiving low or average nutrients from breast milk. All porridges were well received by Mozambican mothers who use cassava as a staple food. The mean scores for sensory liking of all porridges were 3 and above on a five‐point hedonic scale.CONCLUSION: Extrusion‐cooked cassava/soy flour porridges have good potential for use as high‐energy/high‐protein complementary foods and have acceptable sensory properties. Copyright © 2012 Society of Chemical Industry
Antimicrobial activity of food‐compatible plant extracts and chitosan against naturally occurring micro‐organisms in tomato juice
Authors: Giner Manuel J , Vegara Salud , Funes Lorena , Martí Nuria , Saura Domingo , Micol Vicente , Valero Manuel 
Published: January 2012

AbstractBACKGROUND: Chitosan (AC) and five hydroalcoholic extracts from Lithospermum erythrorhizon (SE), Rheum palmatum (RE), Thymus vulgaris (AT), Lippia citriodora (PLX) and a mixture of Rosmarinus officinalis, Salvia lavandulifolia and Thymus mastichina (LA) were tested for antimicrobial activity against bacteria, yeasts and filamentous fungi using two broth dilution methods. The effects of adding single extracts on naturally occurring micro‐organisms and sensory qualities of raw tomato juice were also evaluated.RESULTS: SE extract exhibited the strongest activity, with minimum inhibitory concentrations (MICs) of 100–400 µg mL−1 for Gram‐positive and 1600–3200 µg mL−1 for Gram‐negative bacteria. Enterobacter aerogenes showed the greatest susceptibility to AC (MIC 1600 µg mL−1). Lethal effects of extracts and AC were achieved at a minimum bactericidal concentration (MBC)/MIC ratio of 2 in 88% of assays. SE and RE extracts and AC also exhibited antifungal effect against yeasts, but they had no activity on filamentous fungi. Control and 100 mg L−1 SE‐added tomato juices did not differ in acceptance, but this SE concentration was not effective in the control of microbial load throughout cold storage.CONCLUSION: Results confirm the antimicrobial potential of the plant extracts, but additional research is needed until the agents responsible for the activities have been determined in order to use them as natural constituents of multiple‐barrier food preservation systems. Copyright © 2012 Society of Chemical Industry
Mechanistic aspects of ligand substitution on [(H2O)(tap)2RuORu(tap)2(H2O)]2+ ion {tap = 2‐(m‐tolylazo)pyridine} by some amino acids in aqueous medium at physiological pH
Authors: Mandal Arup , Mondal Subala , Karmakar Parnajyoti , Mallick Subhasis , Bera Biplab K. , Ghosh Alak K. 
Published: January 2012

AbstractThe interaction of the title complex with selected amino acids such as glycine (L1H), l‐valine (L2H), and l‐leucine (L3H) has been studied spectrophotometrically in aqueous medium as a function of [substrate complex], [ligand], and temperature. The reaction has been monitored at 600 nm, where the spectral difference between the reactant and product is maximum. At pH 7.4, the reaction has been found to proceed via two distinct consecutive steps, i.e., it shows a nonlinear dependence on the concentration of ligands: The first process is [ligand] dependent, but the second step is [ligand] independent. The rate constants for the processes are k1∼10−3 s−1 and k2∼10−4 s −1. The activation parameters were calculated from Eyring plots. Based on the kinetic and activation parameters, an associative interchange mechanism is proposed for the interaction processes. From the temperature dependence of the outer sphere association equilibrium constant, the thermodynamic parameters were also calculated, which gives a negative Δ Go value for both the steps at all temperatures studied, supporting the spontaneous formation of an outer sphere association complex. The product of the reaction has been characterized with the help of conductance measurement, IR, NMR, and ESI‐mass spectroscopic analysis. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 612–623, 2012
Sensitivity enhancement of remotely coupled NMR detectors using wirelessly powered parametric amplification
Authors: Qian Chunqi , Murphy‐Boesch Joseph , Dodd Stephen , Koretsky Alan 
Published: January 2012

AbstractA completely wireless detection coil with an integrated parametric amplifier has been constructed to provide local amplification and transmission of MR signals. The sample coil is one element of a parametric amplifier using a zero‐bias diode that mixes the weak MR signal with a strong pump signal that is obtained from an inductively coupled external loop. The NMR sample coil develops current gain via reduction in the effective coil resistance. Higher gain can be obtained by adjusting the level of the pumping power closer to the oscillation threshold, but the gain is ultimately constrained by the bandwidth requirement of MRI experiments. A feasibility study here shows that on a NaCl/D2O phantom, 23Na signals with 20 dB of gain can be readily obtained with a concomitant bandwidth of 144 kHz. This gain is high enough that the integrated coil with parametric amplifier, which is coupled inductively to external loops, can provide sensitivity approaching that of direct wire connection. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.
Flexible transceiver array for ultrahigh field human MR imaging
Authors: Wu Bing , Zhang Xiaoliang , Wang Chunsheng , Li Ye , Pang Yong , Lu Jonathan , Xu Duan , Majumdar Sharmila , Nelson Sarah J. , Vigneron Daniel B. 
Published: January 2012

AbstractA flexible transceiver array, capable of multiple‐purpose imaging applications in vivo at ultrahigh magnetic fields was designed, implemented and tested on a 7 T MR scanner. By alternately placing coil elements with primary and secondary harmonics, improved decoupling among coil elements was accomplished without requiring decoupling circuitry between resonant elements, which is commonly required in high‐frequency transceiver arrays to achieve sufficient element‐isolation during radiofrequency excitation. This flexible array design is capable of maintaining the required decoupling among resonant elements in different array size and geometry and is scalable in coil size and number of resonant elements (i.e., number of channels), yielding improved filling factors for various body parts with different geometry and size. To investigate design feasibility, flexibility, and array performance, a multichannel, 16‐element transceiver array was designed and constructed, and in vivo images of the human head, knee, and hand were acquired using a whole‐body 7 T MR system. Seven Tesla parallel imaging with generalized autocalibrating partially parallel acquisitions (GRAPPA) performed using this flexible transceiver array was also presented. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.
Comparison of two ultrashort echo time sequences for the quantification of T1 within phantom and human Achilles tendon at 3 T
Authors: Wright Peter , Jellus Vladimir , McGonagle Dennis , Robson Matthew , Ridgeway John , Hodgson Richard 
Published: January 2012

AbstractUltrashort echo time (UTE) techniques enable direct imaging of musculoskeletal tissues with short T2 allowing measurement of T1 relaxation times. This article presents comparison of optimized 3D variable flip angle UTE (VFA‐UTE) and 2D saturation recovery UTE (SR‐UTE) sequences to quantify T1 in agar phantoms and human Achilles tendon. Achilles tendon T1 values for asymptomatic volunteers were compared to Achilles tendon T1 values calculated from patients with clinical diagnoses of spondyloarthritis (SpA) and Achilles tendinopathy using an optimized VFA‐UTE sequence. T1 values from phantom data for VFA‐ and SR‐UTE compare well against calculated T1 values from an assumed gold standard inversion recovery spin echo sequence. Mean T1 values in asymptomatic Achilles tendon were found to be 725 ± 42 ms and 698 ± 54 ms for SR‐ and VFA‐UTE, respectively. The patient group mean T1 value for Achilles tendon was found to be 957 ± 173 ms (P < 0.05) using an optimized VFA‐UTE sequence with pulse repetition time of 6 ms and flip angles 4, 19, and 24°, taking a total 9 min acquisition time. The VFA‐UTE technique appears clinically feasible for quantifying T1 in Achilles tendon. T1 measurements offer potential for detecting changes in Achilles tendon due to SpA without need for intravenous contrast agents. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.
On the fluid‐tissue contrast behavior of high‐resolution steady‐state sequences
Authors: Bieri Oliver , Ganter Carl , Scheffler Klaus 
Published: January 2012

AbstractIn general, MR image contrast is expected to be resolution independent, but a pronounced loss of contrast is observed between fluids and tissues with contemporary musculoskeletal protocols (typical inplane resolution << 1 mm) using nonbalanced steady‐state free precession, such as double echo steady state. For nonbalanced steady‐state free precession, diffusion sensitivity increases with increasing spoiler moments which increase with decreasing voxel size, suggesting diffusion damping as the major cause for the observed contrast variation. This is confirmed by simulations and measurements indicating that for fluids, diffusion effects become apparent already for resolutions Δx < 1 mm, whereas tissues typically require Δx < 200 μm. Gradient spoiling, however, is generically not minimized but frequently applied along the readout direction. For anisotropic steady‐state free precession scans, the loss of contrast between fluids and tissues from diffusion can thus be minimized by simply moving the spoiler gradients to the lowest resolution direction. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.
Mitochondria of trained skeletal muscle are protected from deleterious effects of statins
Authors: Bouitbir Jamal , Daussin Frédéric , Charles Anne‐Laure , Rasseneur Laurence , Dufour Stéphane , Richard Ruddy , Piquard François , Geny Bernard , Zoll Joffrey 
Published: January 2012

AbstractIntroduction: Statins are associated with adverse skeletal muscle effects. Our objective was to determine if muscular adaptations following exercise training prevented deleterious effects of atorvastatin in glycolytic skeletal muscle. Methods: Twenty rats were divided into 2 groups: a control group (n = 10; Cont) and a 10 days of training group (n = 10; Training). Using the permeabilized fibers technique, we explored mitochondrial function. Results: Exercise training increased Vmax and H2O2 production without altering the free radical leak, and mRNA expression of SOD2 and Cox1 were higher in trained muscle. In the Cont group, atorvastatin exposure increased H2O2 production and decreased skeletal muscle Vmax. The decreased Vmax effect of atorvastatin was dose dependent. Interestingly, the half‐maximal inhibitory concentration (IC50) was higher in the Training group. H2O2 production increased in trained muscle after atorvastatin exposure. Conclusions: These results suggest that improvements in mitochondrial respiratory and antioxidant capacities following endurance training protected mitochondria against statin exposure. Muscle Nerve 46: 367–373, 2012
Hepatoma‐derived growth factor regulates breast cancer cell invasion by modulating epithelial–mesenchymal transition
Authors: Chen San‐Cher , Kung Mei‐Lang , Hu Tsung‐Hui , Chen Hsuan‐Yu , Wu Jian‐Ching , Kuo Hsiao‐Mei , Tsai Han‐En , Lin Yu‐Wei , Wen Zhi‐Hong , Liu Jong‐Kang , Yeh Ming‐Hsin , Tai Ming‐Hong 
Published: January 2012

AbstractHepatoma‐derived growth factor (HDGF) participates in tumourigenesis but its role in breast cancer is unclear. We set out to elucidate the expression profile and function of HDGF during breast carcinogenesis. Immunoblot and immunohistochemical studies revealed elevated HDGF expression in human breast cancer cell lines and tissues. Nuclear HDGF labelling index was positively correlated with tumour grade, stage and proliferation index, but negatively correlated with survival rate in breast cancer patients. HDGF over‐expression was associated with lymph node metastasis and represented an independent prognostic factor for tumour recurrence. Gene transfer studies were performed to elucidate the influence of cellular HDGF level on the malignant behaviour and epithelial‐mesenchymal transition (EMT) of breast cancer cells. Adenovirus‐mediated HDGF over‐expression stimulated the invasiveness and colony formation of MCF‐7 cells. Moreover, HDGF over‐expression promoted breast cancer cell EMT by E‐cadherin down‐regulation and vimentin up‐regulation. Conversely, HDGF knockdown by RNA interference in MDA‐MB‐231 cells attenuated the malignant behaviour and elicited EMT reversal by enhancing E‐cadherin expression while depleting vimentin expression. Because HDGF is a secreted protein, we evaluated the cellular function of recombinant HDGF and found that exogenously supplied HDGF enhanced the invasiveness of breast cancer cells by down‐regulating E‐cadherin and up‐regulating vimentin at transcriptional and translational levels. In contrast, blockade of HDGF secretion with an HDGF antibody inhibited the malignant behaviours and EMT. Finally, exogenous HDGF partially reversed benzyl isothiocyanate (BITC)‐induced EMT suppression. HDGF over‐expression may exert a prognostic role for tumour metastasis and recurrence in breast cancer by modulating EMT. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Effect of carbon‐based nanoparticles on the cure characteristics and network structure of styrene–butadiene rubber vulcanizate
Authors: Saatchi Mohammad Mahdi , Shojaei Akbar 
Published: January 2012

AbstractThe network structure of styrene–butadiene rubber (SBR) in the presence of carbon black (CB) with two different structures and multi‐walled carbon nanotubes (MWCNTs) was investigated. Swelling behaviour, tensile properties at various strain rates and cure kinetics were characterized. Experimental data were analysed using the Flory–Rehner model as well as the tube model theory. It is found that the network structure of CB‐filled SBR follows a three‐phase composite model including rigid particles, semi‐rigid bound rubber and matrix rubber. This bound rubber is postulated to be critical for the mechanical and deformational properties, development of crosslinking density in matrix rubber and polymer–filler interaction. For MWCNT‐filled SBR, the bound rubber does not show a substantial contribution to the network structure and mechanical performance, and these properties are greatly dominated by the higher aspect ratio and polymer–filler interaction. Additionally it is deduced that the crosslinking density of matrix rubber increases on incorporation of the fillers compared to unfilled matrix rubber. Copyright © 2012 Society of Chemical Industry