bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aquatic predicted no-effect-concentration derivation for perfluorooctane sulfonic acid
Authors: Qi Ping , Wang Ying , Mu Jingli , Wang Juying 
Published: January 2011

AbstractPerfluorooctane sulfonic acid (PFOS), a representative perfluorinated surfactant, is an anthropogenic pollutant detected in various environmental and biological matrices. Some laboratory and field work has been conducted to assess the aquatic toxicity of PFOS, but little is known regarding its toxicity threshold to the aquatic ecosystem. In the present study, predicted no-effect concentrations (PNECs) were derived by four different approaches. The interspecies correlation estimation (ICE) program and final acute-to-chronic ratio (FACR) were applied to the development of PNEC based on the toxic mode of action (MOA) of PFOS. By comparison of the different PNECs, the recommended aquatic toxicity thresholds for PFOS are in the range of 0.61 to 6.66 µg/L. Based on comparison of PNEC values, microcosm results, and reported environmental concentrations, PFOS appears not to pose a serious threat to aquatic organisms. The present results demonstrate that MOA is an important consideration for the derivation of reliable PNECs; moreover, the ICE-based species sensitivity distribution (SSD) method can be used to derive PNECs when toxicological data are limited. The application of MOA and ICE for deriving PNEC values in the present study may facilitate studies on using a combination of quantitative structure–activity relationship (QSAR) models and ICE to estimate PNECs. Environ. Toxicol. Chem. 2011; 30:836–842. © 2010 SETAC
Lapatinib inhibits receptor phosphorylation and cell growth and enhances antibody‐dependent cellular cytotoxicity of EGFR‐ and HER2‐overexpressing esophageal cancer cell lines
Authors: Mimura Kousaku , Kono Koji , Maruyama Takanori , Watanabe Mitsuaki , Izawa Shinichiro , Shiba Shugo , Mizukami Yoshiki , Kawaguchi Yoshihiko , Inoue Masayuki , Kono Tetsuo , Choudhury Aniruddha , Kiessling Rolf , Fujii Hideki 
Published: January 2011

AbstractLapatinib is a dual tyrosine kinase inhibitor of the EGFR and HER2 tyrosine kinase domains. EGFR is expressed in 33.3% and HER2 in 30.3% of esophageal squamous cell carcinomas (ESCCs). To explore the potential utility of Lapatinib for therapy of ESCC patients, we evaluated the effect of Lapatinib on a panel of ESCC cell lines. EGFR and HER2 expression by the cell lines was established, and the effects of Lapatinib on inhibition of the phosphorylation of HER2, antiproliferative effect, apoptosis‐inducing activity and accumulation of HER2 and EGFR on cell surface were evaluated. Additionally, the combined effect of Lapatinib together with Herceptin or Cetuximab on cell‐mediated cytotoxicity was evaluated. Lapatinib inhibited HER2 phosphorylation in HER2‐overexpressing, HER2 gene amplification positive ESCC cell line. Lapatinib also inhibited cell proliferation, induced apoptosis and caused the surface accumulation of HER2 and EGFR in ESCC cell lines. Addition of Lapatinib increased Herceptin‐mediated antibody‐dependent cell‐mediated cytotoxicity by 15–25% with three ESCC target cell lines. Similarly, Cetuximab‐mediated antibody‐dependent cell‐mediated cytotoxicity also increased by 15–30% in two ESCC cell lines on addition of Lapatinib. Cumulatively, the data indicate that Lapatinib has activity in EGFR‐ and/or HER2‐expressing ESCC cells, and the combination therapy of Lapatinib and Cetuximab/Herceptin is a promising strategy in ESCC.
Glutamate increases pancreatic cancer cell invasion and migration via AMPA receptor activation and Kras‐MAPK signaling
Authors: Herner Alexander , Sauliunaite Danguole , Michalski Christoph W. , Erkan Mert , Oliveira Tiago De , Abiatari Ivane , Kong Bo , Esposito Irene , Friess Helmut , Kleeff Jörg 
Published: January 2011

AbstractGlutamate has been implicated in tumorigenesis through activation of alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptors (AMPAR). However, the function of a glutamate‐to‐AMPAR signal in pancreatic ductal adenocarcinoma (PDAC) has remained elusive. We now show that glutamate‐mediated AMPA receptor activation increases invasion and migration of pancreatic cancer cells via activation of the classical MAPK pathway. Glutamate levels were increased in pancreatic cancer accompanied by downregulation of GluR subunits 1, 2, and 4. In pancreatic cancer precursor lesions, pancreatic intraepithelial neoplasia (PanIN), GluR1 subunit levels were strikingly and step‐wise increased but its expression was rare in PDAC. Pharmacological inhibition or RNAi‐mediated suppression of GluR1 or GluR2 did not affect cancer cell growth but significantly decreased invasion. In a K‐ras wildtype cell line, AMPA receptor activation enhanced K‐ras activity and—further downstream—phosphorylation of p38 and of p44/42. Preemptive blockade of AMPA receptors in a mouse model of pancreatic cancer inhibited tumor cell settling. AMPA receptor activation thus not only activates MAPK signalling but also directly increases activity of K‐ras. Glutamate might serve as a molecular switch that decreases the threshold of K‐ras‐induced oncogenic signalling and increases the chance of malignant transformation of pancreatic cancer precursor lesions.
The spectrum of malignancies in HIV‐infected patients in 2006 in France: The ONCOVIH study
Authors: Lanoy Emilie , Spano Jean‐Philippe , Bonnet Fabrice , Guiguet Marguerite , Boué François , Cadranel Jacques , Carcelain Guislaine , Couderc Louis‐Jean , Frange Pierre , Girard Pierre‐Marie , Oksenhendler Eric , Poizot‐Martin Isabelle , Semaille Caroline , Agut Henri , Katlama Christine , Costagliola Dominique ,   the ONCOVIH study group
Published: January 2011

AbstractSince no large descriptive studies of incident cancers in HIV‐infected patients are available in France, the nationwide cross‐sectional ONCOVIH study aimed to prospectively report new malignancies diagnosed in HIV‐infected patients in cancer centers and HIV/AIDS centers. We estimated the number of cancers in France for the year 2006 using the capture–recapture methods with two sources: ONCOVIH and the FHDH ANRS‐CO4 cohort, as well as the completeness of the sources. Incidence and relative risks (RR) to the general population were estimated. In 2006, 672 new malignancies in 668 patients were reported in ONCOVIH; the most common were non Hodgkin's lymphoma (NHL, 21.5%), Kaposi's sarcoma (KS, 16.0%), lung cancer (9.4%), anal cancer (8.2%), Hodgkin's lymphoma (7.6%), skin cancers excluding melanoma (6.8%), and liver cancer (5.6%). Based on the capture‐recapture approach, the estimated number of malignancies was 1320 and non‐AIDS‐defining malignancies (NADM) represented 68% of cases. The overall ascertainment of malignancies were 53%, and 59%, in the ONCOVIH study, and the FHDH ANRS‐CO4 cohort, respectively. The estimated incidence of cancer among HIV‐infected patients was 14 per 1000 person‐years. Compared with the general population, the estimated RR in HIV–infected patients was 3.5 (95%CI 3.3–3.8) in men and 3.6 (95%CI 3.2–4.0) in women, and was particularly elevated in younger patients. Even in the era of combined antiretroviral therapy, the incidence of cancer is higher in HIV‐infected persons than in the general population. A large variety of malignancies were diagnosed, and the majority were NADM.
Regulatory BCL2 promoter polymorphism (−938C>A) is associated with adverse outcome in patients with prostate carcinoma
Authors: Bachmann Hagen S. , Heukamp Lukas C. , Schmitz Klaus J. , Hilburn Caroline F. , Kahl Philip , Buettner Reinhard , Nückel Holger , Eisenhardt Andreas , Rübben Herbert , Schmid Kurt Werner , Siffert Winfried , Eggert Angelika , Schramm Alexander , Schulte Johannes H. 
Published: January 2011

AbstractMolecular markers predictive of prostate cancer prognosis are urgently needed. Overexpression of the antiapoptotic protein, Bcl‐2, has repeatedly been shown to be associated with adverse outcome in this malignancy. We hypothesized that a regulatory BCL2 −938C>A promoter polymorphism, which significantly affects promoter activity and Bcl‐2 expression in different malignancies, may influence survival. Reporter assays and electrophoretic mobility shift assays reveled that the −938C>A BCL2 promoter polymorphism significantly affects promoter activity and transcription factor binding in prostate cancer cells. Significantly higher BCL2 mRNA expression was observed in primary prostate carcinomas derived from patients with the AA, compared to CC, genotype. Survival analysis showed that the −938AA genotype was an independent, unfavorable prognostic factor for relapse‐free survival in a primary cohort of 142 patients and in an independent replication cohort of 148 patients, with hazard ratios (HR) of 4.4 (95% CI, 1.3–15.1; p = 0.018) and 4.6 (95% CI, 1.5–14.2; p = 0.009). Furthermore, the −938AA genotype was independently associated with worse overall survival in the replication series, with a HR of 10.9 (95% CI, 1.2–99.3; p = 0.034). We conclude that the BCL2 −938C>A polymorphism is an independent predictor of relapse‐free and overall survival in patients with prostate cancer. The BCL2 −938C>A polymorphism should be evaluated prospectively and may also have promise in assisting optimal patient choice for treatment with BCL2‐targeted drugs already in evaluation for prostate cancer treatment.
The association of body mass index with mortality in the California Teachers Study
Authors: Bessonova Leona , Marshall Sarah F. , Ziogas Argyrios , Largent Joan , Bernstein Leslie , Henderson Katherine D. , Ma Huiyan , West Dee W. , Anton‐Culver Hoda 
Published: January 2011

AbstractAlthough underweight and obesity have been associated with increased risk of mortality, it remains unclear whether the associations differ by hormone therapy (HT) use and smoking. The authors examined the relationship between body mass index (BMI) and mortality within the California Teachers Study (CTS), specifically considering the impact of HT and smoking. The authors examined the associations of underweight and obesity with risks of all‐cause and cause‐specific mortality, among 115,433 women participating in the CTS, and specifically examined whether HT use or smoking modifies the effects of obesity. Multivariable Cox proportional hazards regression provided estimates of relative risks (RRs) and 95% confidence intervals (CIs). During follow up, 10,574 deaths occurred. All‐cause mortality was increased for underweight (BMI <18.5; adjusted RR = 1.33, 95% CI = 1.20–1.47) and obese participants (BMI ≥ 30: RR = 1.27, 95% CI = 1.19–1.37) relative to BMI of 18.5–24.9). Respiratory disease mortality was increased for underweight and obese participants. Death from any cancer, and breast cancer specifically, and cardiovascular disease was observed only for obese participants. The obesity and mortality association remained after stratification on HT and smoking. Obese participants remained at greater risk for mortality after stratification on menopausal HT and smoking. Obesity was associated with increased all‐cause mortality, as well as death from any cancer (including breast), and cardiovascular and respiratory diseases. These findings help to identify groups at risk for BMI‐related poor health outcomes.
High expression of stratifin is a universal abnormality during the course of malignant progression of early‐stage lung adenocarcinoma
Authors: Shiba‐Ishii Aya , Kano Junko , Morishita Yukio , Sato Yukio , Minami Yuko , Noguchi Masayuki 
Published: January 2011

AbstractAdenocarcinoma in situ (AIS) of the lung has an extremely favorable prognosis, with a 5‐year survival rate of 100%. However, early invasive adenocarcinoma (EIA) often has a fatal outcome. In this study, we compared the expression profiles of AIS with those of EIA showing lymph node metastasis or a fatal outcome, and screened the differentially expressed genes by cDNA microarray. From the genes selected, we focused on Stratifin (SFN, 14‐3‐3 σ), which showed significantly higher expression in EIA than in AIS. Immunohistochemistry for SFN revealed that more than 95% of EIAs were immunopositive for SFN, in comparison to only 13% of AISs (p <0.05). Interestingly, positivity was detected not only in the invasive region but also in the in situ spreading component of EIA. Functionally, SFN facilitates the cell proliferation capacity of lung adenocarcinoma. These results indicate that SFN overexpression is a universal abnormality during the stepwise progression from in situ to invasive adenocarcinoma of the lung.
Association of tissue promoter methylation levels of APC, TGFβ2, HOXD3 and RASSF1A with prostate cancer progression
Authors: Liu Liyang , Kron Ken J. , Pethe Vaijayanti V. , Demetrashvili Nino , Nesbitt Michael E. , Trachtenberg John , Ozcelik Hilmi , Fleshner Neil E. , Briollais Laurent , van der Kwast Theodorus H. , Bapat Bharati 
Published: January 2011

AbstractAberrant promoter methylation is known to silence tumor‐suppressor genes in prostate cancer (PCa). We correlated quantitative promoter methylation levels of APC, TGFβ2 and RASSF1A in 219 radical prostatectomies diagnosed between 1998 and 2001 with clinicopathological follow‐up data available including Gleason Pattern (GP), Gleason Score (GS) and pathological stage and explored their potential in predicting biochemical recurrence using univariate and multivariate analyses. We observed that the average methylation levels of APC increased significantly from GS ≤ 6 to GS7, and pT2 to pT3a, and that of TGFβ2 increased from GS ≤ 6 to GS7, but not for RASSF1A. PCa samples were also stratified into high methylation (HM) and low methylation (LM) groups based on the PMR scores of all cases analyzed for each marker. The HM frequency of APC was greater in pT3a than pT2, and in GS ≥ 8 than GS ≤ 6. The HM frequency also increased significantly from GP3 to GP4 for APC, TGFβ2 and RASSF1A. APC methylation level was a significant predictor of biochemical recurrence in univariate analysis (p‐value = 0.028). Finally, we combined methylation data of these three genes with the previously reported novel methylation biomarker HOXD3. Quantitative methylation assessment of a multiplex panel of markers, consisting of APC, HOXD3 and TGFβ2, outperforms any single marker for the prediction of biochemical recurrence (p‐value = 0.017). Our study demonstrated that quantitative increase in promoter methylation levels of APC, HOXD3 and TGFβ2 are associated with PCa progression.
Eurogin 2010 roadmap on cervical cancer prevention
Authors: Franceschi Silvia , Denny Lynette , Irwin Kathleen L. , Jeronimo José , Lopalco Pier Luigi , Monsonego Joseph , Peto Julian , Ronco Guglielmo , Sasieni Peter , Wheeler Cosette M. 
Published: January 2011

AbstractThe EUROGIN 2010 roadmap represents a continuing effort to provide and interpret updated information on cervical cancer screening and vaccination against the cause of the disease, high‐risk human papillomavirus (HPV). Contrary to the two previous reports in 2008 and 2009, the present roadmap gives equal room to HPV‐based screening and HPV vaccination, as a result of the recent strengthening of the evidence on the efficacy and feasibility of both approaches. The superiority of HPV testing in primary screening compared to cytology (in more developed countries) and to cytology or visual inspection methods (in less developed countries) has been demonstrated in several randomised trials. High vaccine efficacy has been confirmed up to 7 years after vaccination; school‐based programmes in some countries have been able to reach over 70% coverage among adolescent girls. Demonstration projects have indicated that the delivery of HPV vaccines in less developed countries is feasible and favourably received by populations where cervical cancer is very common. HPV‐based screening can diminish cervical cancer incidence more quickly than HPV vaccination, but vaccination can eventually facilitate screening efforts, especially if new vaccines against a greater number of HPV types are introduced. The availability of two highly complementary prevention tools such as HPV testing and HPV vaccination makes it possible to conceive integrated strategies for the elimination of cervical cancer that have no precedent in the cancer field. HPV tests and HPV vaccines remain, however, too expensive, and large‐scale financing of screening and vaccination in less developed countries is sorely lacking.
Surface chemical patterning for long‐term single‐cell culture
Authors: Cheng Qian , Komvopoulos Kyriakos , Li Song 
Published: January 2011

AbstractSurface chemical patterning of polystyrene (PS) dishes for long‐term single‐cell culture was accomplished by oxygen plasma treatment through the windows of a polydimethylsiloxane membrane mask that produced hydrophilic areas of different shapes and sizes, followed by overnight incubation with either Pluronic F108 solution or a mixture of Pluronic F108 solution and fibronectin. Selective cell attachment on pattern areas of PS dishes was investigated in light of cell seeding experiments and X‐ray photoelectron spectroscopy measurements. Activation of the hydrophilic areas of patterned PS surfaces by serum proteins in the culture medium was conducive to cell attachment on the pattern areas of dishes incubated with only Pluronic solution. Preferential adsorption of fibronectin on hydrophilic pattern areas enhanced selective cell attachment on patterned dishes incubated with a mixture of Pluronic solution and fibronectin. Cell‐culture experiments demonstrated an effect of surface patterning on both cell and nucleus shape and confirmed the long‐term (>2 weeks) stability of the produced single‐cell patterns in serum medium. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
PLGA‐chitosan/PLGA‐alginate nanoparticle blends as biodegradable colloidal gels for seeding human umbilical cord mesenchymal stem cells
Authors: Wang Qun , Jamal Syed , Detamore Michael S. , Berkland Cory 
Published: January 2011

AbstractThe natural polymers chitosan and alginate represent an attractive material choice for biodegradable inplants. These were used as coating materials to make positively and negatively charged PLGA nanoparticles, respectively. After blending at total solids concentration >10% wt/vol, these oppositely charged nanoparticles yielded a cohesive colloidal gel. Electrostatic forces between oppositely charged nanoparticles produced a stable 3D porous network that may be extruded or molded to the desired shape. This high concentration colloidal system demonstrated shear‐thinning behavior due to the disruption of interparticle interactions. Once the external force was removed, the cohesive property of the colloidal gel was recovered. Scanning electron micrographs of dried colloidal networks revealed an organized, 3D microporous structure. Rheological studies were employed to probe the differences in plasticity and shear sensitivity of colloidal gels. Viability tests of hUCMSCs seeded on the colloidal gels also demonstrated the negligible cytotoxicity of the materials. All the results indicated the potential application of the biodegradable colloidal gels as an injectable scaffold in tissue engineering and drug release. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Adhesion and growth of dental pulp stem cells on enamel‐like fluorapatite surfaces
Authors: Liu J. , Jin T. C. , Chang S. , Czajka‐Jakubowska A. , Clarkson B. H. 
Published: January 2011

AbstractTo study how apatite crystal alignment of an enamel‐like substrate affects DPSC cellular adhesion and growth as a precursor to produce an in vitro enamel/dentin superstructure for future studies. The cells were subcultured in 10% FBS DMEM up to seven weeks on the two surfaces. Specimens were observed under SEM, counted, and analyzed using the human pathway‐focused matrix and adhesion PCR array. After three days, the cell number on ordered FA surface was significantly higher than on the disordered surface. Of the 84 focused pathway genes, a total of 20 genes were either up or down regulated in the cells on ordered FA surface compared to the disordered surface. More interestingly, of the cell‐matrix adhesion molecules, integrin alpha 7 and 8 (ITGA 7 and 8), integrin beta 3 and 4 (ITGB3 and 4), and the vitronectin receptor‐integrin alpha V (ITGAV) and the key adhesion protein‐fibronectin1 (FN1) were up‐regulated. In SEM, both surfaces showed good biocompatibility and supported long term growth of DPSC cells but with functional cell‐matrix interaction on the ordered FA surfaces. Significance: The enhanced cellular response of DPSC cell to the ordered FA crystal surface involves a set of delicately regulated matrix and adhesion molecules which could be manipulated by treating the cells with a dentin extract, to produce a dentin/enamel superstructure. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Influence of polysaccharide composition on the biocompatibility of pullulan/dextran‐based hydrogels
Authors: Abed Aicha , Assoul Nabila , Ba Maguette , Derkaoui Sidi Mohamed , Portes Patrick , Louedec Liliane , Flaud Patrice , Bataille Isabelle , Letourneur Didier , Meddahi‐Pellé Anne 
Published: January 2011

AbstractThe implantation of a biomaterial for tissue engineering requires the presence of a suitable scaffold on which the tissue repair and regeneration will take place. Polymers have been frequently used for that purpose because they show similar properties to that of the natural extracellular matrix. Scaffold properties and biocompatibility are modulated by the composition of the polymers used. In this work four polysaccharide‐based hydrogels (PSH) made of dextran and pullulan were synthesized. Theirin vitroproperties were determined and then testedin vivoin a rat model. As pullulan concentration increased in dextran hydrogels, the glass transition temperature and the maximum modulus decreased.In vitrodegradation studies for 30 days demonstrated no significant degradation of PSH except for 100% pullulan hydrogel.In vivotissue response evaluated 30 days after PSH subcutaneous implantation in rats indicated that all PSH were surrounded by a fibrous capsule. Adding pullulan to dextran induced an increased inflammatory reaction compared to PSH‐D (100% dextran) or PSH‐D75P25(75% dextran).Thisin vitroandin vivodata can be used in the design of hydrogels appropriate for tissue engineering applications © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Osteoprotegerin deficiency attenuates strontium-mediated inhibition of osteoclastogenesis and bone resorption
Authors: Peng Songlin , Liu X Sherry , Zhou Guangqian , Li Zhaoyang , Luk Keith DK , Guo X Edward , Lu W William 
Published: January 2011

Abstract
Strontium (Sr) exerts an anabolic and antiresorptive effect on bone, but the mechanism remains unknown. Osteoprotegerin (OPG) expressed by osteoblasts plays an important role in regulating bone homeostasis by inhibiting osteoclastogenesis and bone resorption. This study aims at evaluating the role of OPG in Sr-mediated inhibition of osteoclastogenesis and bone resorption. Six-week-old Opg knockout (KO) male mice and their wild-type (WT) littermates were treated orally with vehicle (Veh) or Sr compound (4 mmol/kg) daily for 8 weeks. Bone mass and microstructure in the lumbar spine (L4) and proximal tibia were analyzed with micro–computed tomography (µCT). Bone remodeling was evaluated with serum biochemical analysis and static and dynamic bone histomorphometry. Osteoclast differentiation potential and gene expression were analyzed in bone marrow cells. The findings demonstrate that Sr compound treatment results in greater bone volume and trabecular number than Veh treatment in WT mice. The anabolic response of trabecular bone to Sr treatment is attenuated in KO mice. Although Sr treatment significantly decreases in vitro osteoclastogenesis and bone resorption in WT mice, these effects are attenuated in KO mice. Furthermore, Sr treatment profoundly increases Opg gene expression in the tibias and OPG protein levels in the sera of WT mice. This study concludes that the inhibition of osteoclastogenesis and bone resorption is possibly associated with OPG upregulation by Sr treatment. © 2011 American Society for Bone and Mineral Research.