bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional activity analysis of promoter region of human PAX9 gene under dexamethasone, retinoic acid, and ergocalciferol treatment in MCF‐7 and MDPC23
Authors: Ramenzoni Liza L. , Saito Cristiane P.B. , McCormick J. Justin , Line Sérgio R.P. 
Published: October 2010

AbstractPAX9 gene is a member of the family homeobox of transcription factors and performs important function in development and organogenesis. Mutations in PAX9 coding sequences have been implicated in autosomal dominant oligodontia affecting predominantly permanent molars and second premolars. Previous studies have shown that PAX9 is required for secondary palate development and teratogens have been identified as inducers of a tooth and craniofacial malformations. This work focused on the analysis on the 5′‐flanking region of the PAX9 gene studying the influence of retinoic acid, dexamethasone, and vitamin D on the expression of PAX9 by expression constructs that carry the reporter gene luciferase. As results, retinoic acid and dexamethasone showed progressive decrease of PAX9 expression. PAX9‐pGL3B1 and PAX9‐pGL3B2 promoter was inhibited under the treatment of dexamethasone and ergocalciferol. Retinoic acid and dexamethasone did not alter PAX9‐pGL3B3 behavior indicating that sequences present between −1106 and +92 were important for the transcriptional activity of PAX9 promoter. In this study, we characterized the transcriptional activity of specific regions of the PAX9 promoter gene and we demonstrated that retinoic acid and ergocalciferol can modulate the transcriptional activity of PAX9 gene. Copyright © 2010 John Wiley & Sons, Ltd.
sFas levels increase in response to cisplatin‐based chemotherapy in lung cancer patients
Authors: Ulukaya Engin , Acilan Ceyda , Yilmaz Meryem , Yilmaztepe‐Oral Arzu , Ari Ferda , Zik Berrin , Ursavas Ahmet , Tokullugil Asuman H. 
Published: October 2010

AbstractThe Fas/Fas Ligand (FasL) system and survivin have counteracting roles in cell survival. Therefore, we explored the role of circulating soluble Fas (sFas) and the tissue levels of Fas and survivin with regard to response to chemotherapy in lung cancer patients. Serum samples from 52 lung cancer patients and 54 control subjects (19 benign lung disease and 35 healthy control subjects) were collected prior to and 24 and 48 h after chemotherapy. sFas was statistically significantly higher in the cancer group than that in the control groups (p < 0.001). Baseline (before chemotherapy) sFas values showed a statistically significant inverse correlation with overall survival (r = −0.599, p < 0.001). There was a significant increase in serum sFas levels 24 h after treatment (p < 0.05). Contrarily, tissue levels of Fas and survivin were not changed following the chemotherapy (p > 0,05). In conclusion, increased sFas may be an indicator of poor outcome in lung cancer patients. However, cisplatin‐based chemotherapy may not be effective via neither the Fas/FasL system nor survivin pathway. Indeed, larger sample size is required for further evaluation. Copyright © 2010 John Wiley & Sons, Ltd.
Association study between methylenetetrahydrofolate reductase gene polymorphisms and Graves' disease
Authors: Mao Renfang , Fan Yihui , Zuo Lulu , Geng Dongfeng , Meng Fantao , Zhu Jing , Li Qiang , Qiao Hong , Jin Yan , Bai Jing , Fu Songbin 
Published: October 2010

Abstract5,10‐Methylenetetrahydrofolate reductase (MTHFR) catalyzes the metabolism of folate and nucleotides, which are essential for DNA synthesis and methylation. It is highly polymorphic, and its variant genotypes result in lower enzymatic activity and higher plasma homocysteine. Previous studies have provided evidence that a high prevalence of MTHFR gene polymorphisms is frequently detected in patients with autoimmune disease, suggesting a novel genetic association with autoimmune disorders. However, the genetic association between MTHFR and Graves' disease (GD), one of the most common autoimmune diseases, has not been studied. Here, we designed a clinic‐based case–control study including 199 GD cases and 235 healthy controls to examine the associations between three common MTHFR polymorphisms (i.e., C677T, A1298C, and G1793A) and GD. Surprisingly, logistic regression analysis shows MTHFR 677CT + TT genotypes are associated with an approximately 42% reduction in the risk of GD in women (adjusted OR = 0.58, 95% CI = 0.3–0.9), compared to the CC genotype, indicating a significant protective effect of 677CT + TT genotypes. Our result provides epidemiological evidence that MTHFR mutation (C677T) protects women from GD. The protective effect, possibly obtained by influencing DNA methylation, should be confirmed in a large number of cohorts. Copyright © 2010 John Wiley & Sons, Ltd.
Association analysis of the E‐selectin 98G > T polymorphism and the risk of childhood ischemic stroke
Authors: Sarecka‐Hujar Beata , Zak Iwona , Emich‐Widera Ewa , Kopyta Ilona , Pilarska Ewa , Pienczk‐Reclawowicz Karolina 
Published: October 2010

AbstractGenes related to platelet and arterial endothelial function have been recently considered as independent risk factors for stroke. We aimed to analyze a relationship between the E‐selectin 98G > T polymorphism and stroke in children and to observe the transmission of E‐selectin alleles from heterozygous parents to their affected children. We studied 59 children after stroke, 112 parents, and 87 healthy children. The E‐selectin 98G > T polymorphism was analyzed with the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method. The frequency of the 98T allele in patients was almost twofold lower than in controls (5.1% vs. 9.8%, p = 0.145, odds ratios (OR) = 0.49) as well as carriers of the 98T allele (19.5% in controls vs. 8.5% in cases, p = 0.067, OR = 0.38). The G allele of the E‐selectin 98G > T polymorphism was more frequently transmitted to the children after stroke compared to the T allele (68% vs. 32%). In conclusion, we did not confirm the relationship between the 98G > T polymorphism of the E‐selectin gene and childhood ischemic stroke. There is still a need for further studies. Copyright © 2010 John Wiley & Sons, Ltd.
An EF‐hands protein, centrin‐1, is an EGTA‐sensitive SUMO‐interacting protein in mouse testis
Authors: Tanaka Niina , Goto Miki , Kawasaki Azusa , Sasano Takashi , Eto Ko , Nishi Ryotaro , Sugasawa Kaoru , Abe Shinichi , Saitoh Hisato 
Published: October 2010

AbstractA multifunctional calcium‐binding protein, centrin‐1, is specifically expressed in male germ cells, certain neurons and ciliated cells. We identified centrin‐1 as a protein interacting with SUMO‐2/3 using yeast two‐hybrid screening of a mouse testicular cDNA library. In bead halo assays, the interaction between centrin‐1 and SUMO‐2/3 was reduced in the presence of EGTA and facilitated by the addition of CaCl2. immunostaining of seminiferous tubules in 35‐day‐old mouse testes revealed that cells in the layer containing spermatogonia showed colocalization of SUMO‐2/3 with centrin‐1 in cytoplasmic spots. Identification of centrin‐1 as the EGTA‐sensitive SUMO‐2/3‐interacting protein indicates the possible role of calcium in modulating the centrin‐1–SUMO‐2/3 interaction and suggests the importance of this interaction in mouse testis. Copyright © 2010 John Wiley & Sons, Ltd.
Development of an Activity‐Based Probe for Autotaxin
Authors: Cavalli Silvia , Houben Anna J. S. , Albers Harald M. H. G. , van Tilburg Erica W. , de Ru Arnoud , Aoki Junken , van Veelen Peter , Moolenaar Wouter H. , Ovaa Huib 
Published: October 2010

AbstractAutotaxin (ATX), or ecto‐nucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), is a secreted lysophospholipase D that hydrolyses lysophosphatidylcholine into the lipid mediator lysophosphatidic acid (LPA), a mitogen and chemoattractant for many cell types. ATX has been implicated in tumour progression and inflammation, and might serve as a biomarker. Here we describe the development of a fluorescent activity‐based probe that covalently binds to the active site of ATX. The probe consists of a lysophospholipid‐based backbone linked to a trapping moiety that becomes reactive after phosphate ester hydrolysis, and a Cy5 fluorescent dye to allow visualisation of active ATX. The probe reacts specifically with the three known isoforms of ATX, it competes with small‐molecule inhibitors for binding to ATX and allows ATX activity in plasma to be determined. Our activity‐based reporter will be useful for monitoring ATX activity in biological fluids and for inhibitor screening.
Selective Adsorbents Enhancing Exhaust Air Treatment for the Food‐Processing Industry
Authors: Täffner T. , Niemeyer B. , Liu M.Sc. Y. , Bischoff M. , Birkenseer M. 
Published: October 2010

AbstractEs werden Methoden und Kriterien zur Auswahl und Charakterisierung spezifisch wirkender Adsorbentien vorgestellt. Die Untersuchungen sollen ein neues multivariates Clusterverfahren für Adsorptive verifizieren, das gegenüber den sonst üblichen Screeningverfahren den zeitlichen und materiellen Aufwand zur Auswahl optimierter Adsorbentien minimiert. Die Bedeutung der Adsorption von Wasserdampf und die Einflussmöglichkeiten durch Modifikationen der Adsorbentien werden vorgestellt. Abschließend werden die wirtschaftlichen und technischen Vorteile adsorptiver Trennverfahren zur Abluftbehandlung in lebensmittelverarbeitenden Betrieben diskutiert.
Human papillomavirus genotype detection from archival papanicolaou‐stained cervical tests
Authors: Tabrizi Sepehr N. , Taylor Nicole , McCullough Michael J. , Phillips Gillian , Wark John , Gertig Dorota , Petersen Rodney W. , Saville Marion , Garland Suzanne M. 
Published: October 2010

AbstractBACKGROUND:Archival Papanicolaou (Pap)‐stained cervical cytology tests may be the only source of a clinical sample for the evaluation of previous human papillomavirus (HPV) infection. Pap tests are ideal because the majority of women in countries with comprehensive screening programs would have had several collected and stored.METHODS:In the current study, HPV detection and genotyping were compared in samples collected from a conventionally fixed Pap test with those collected using an endocervical brush and collected in PreservCyt (liquid‐based) in 87 women undergoing management for a high‐grade Pap test abnormality. Cytology slides were scanned to create high‐resolution digital images before the removal of cells because the DNA extraction process resulted in the destruction of the cells from the original sample.RESULTS:All previously identified high‐grade abnormalities on the Pap tests were detectable on the digital images. β‐globin was detected in all extracted Pap tests, indicating the presence of recoverable, amplifiable DNA. A total of 62 (71.3%) and 59 (67.8%) tests were found to have high‐risk (HR) HPV detected on PreservCyt and fixed Pap test slides, respectively, with >87% concordance for the detection of HR HPV genotypes. Complete HPV genotyping concordance was observed in 62% and was partial in 26% of sample pairs, with very good agreement for HPV types 16 and 18 (κ = 0.850 and 0.903, respectively). Only 1 Pap test slide was found to be positive whereas the PreservCyt had no detectable HPV DNA, demonstrating a low false‐positive rate (1%).CONCLUSIONS:The results of the current study confirm that imaging and subsequent HPV detection and genotyping in archival cervical smears can offer accuracy in HPV detection that is comparable to endocervical brush‐collected PreservCyt samples. Cancer (Cancer Cytopathol) 2010;. © 2010 American Cancer Society.
Origin of adult‐type pigment cells forming the asymmetric pigment pattern, in Japanese flounder (Paralichthys olivaceus)
Authors: Yamada Toshiyuki , Okauchi Masanori , Araki Kazuo 
Published: October 2010

AbstractThe flatfish‐specific asymmetric pigment pattern depends on the asymmetric appearance of adult‐type pigment cells after the late metamorphic stages. To understand the mechanism enabling the formation of this asymmetric pattern, we investigated the behavior of pigment cell latent precursors in postembryonic Japanese flounder, Paralichthys olivaceus, by analysis of the expression patterns of pigment lineage markers (colony stimulating factor 1 receptor, dopachrome tautomerase, kit) and the DiI (DiO) labeling test for latent precursors. We found that, throughout the larval stages, pigment cell latent precursors were predominantly localized along the dorsal and ventral margins of the flank symmetrically and migrated continuously from these regions to the lateral sides symmetrically, and after late metamorphic stages these precursors differentiated into adult‐type pigment cells on the lateral side asymmetrically. We conclude that adult‐type pigment cells that form the asymmetric pigment pattern are continuously derived from the dorsal and ventral margins of the flank during larval development. Developmental Dynamics 239:3147–3162, 2010. © 2010 Wiley‐Liss, Inc.
A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression
Authors: Montazer‐Torbati Fatemeh , Kocer Ayhan , Auguste Aurélie , Renault Lauriane , Charpigny Gilles , Pailhoux Eric , Pannetier Maëlle 
Published: October 2010

AbstractThe testis‐determining gene SRY is not well‐conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital‐ridges and not solely in pre‐Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324–3335, 2010. © 2010 Wiley‐Liss, Inc.
NCN Chelated Organoantimony(III) and Organobismuth(III) Phosphinates and Phosphites: Synthesis, Structure and Reactivity
Authors: Svoboda Tomáš , Jambor Roman , Růžička Aleš , Padělková Zdeňka , Erben Milan , Dostál Libor 
Published: October 2010

AbstractThe reaction of the organoantimony and organobismuthoxides [LSbO]2 (1) and [LBiO]2 (2), where L = [2,6‐(Me2NCH2)2C6H3]–, with phenylphosphinic acid in a 1:4 molar ratio gave the molecular organoantimony and organobismuth phosphinates LM[OP(H)(O)(Ph)]2 [M = Sb (3), Bi (4)]. Similarly, the reaction of 1 and 2 with H3PO3 gave the secondary phosphites LM[OP(H)(O)(OH)]2 [M = Sb (5), Bi (6)] or the fully deprotonated phosphite {LM[O2P(H)(O)]}2 [M = Sb (7), Bi (8)] depending on whether the molar ratio of the starting materials was 1:4 or 1:2. The syntheses of novel mixed phosphinate‐phosphite LSb[OP(H)(O)(OH)][OP(H)(O)(Ph)] (9), phosphonate‐phoshinate LSb[OP(tBu)(O)(OH)][OP(H)(O)(Ph)] (10) and phosphonate‐phosphite LSb[OP(tBu)(O)(OH)][OP(H)(O)(OH)] (11) compounds are also described. While the organoantimony compounds 3, 5 and 7 are stable in solution, the organobismuth congeners showed only limited stability in solution and underwent a redox process involving to the reduction of the bismuth ions, and oxidation of the phosphorus ions from the +III to +V oxidation state. The reaction pathway associated with the redox process was studied with the system bismuth oxide 2 + Ph2P(O)(H), which gave compound LBi[OP(O)(Ph)2]2 (14) as a result of the oxidation of the phosphorus ion. All compounds were characterized by elemental analysis, 1H, 13C and 31P NMR spectroscopy and IR spectroscopy. The solid‐state molecular structures of compounds 3, 5a {LM[O2P(H)(O)][(HO)2P(H)(O)]} and 7 were determined by X‐ray diffraction.
A Novel Water‐Soluble Hexanuclear Bismuth Oxido Cluster – Synthesis, Structure and Complexation with Polyacrylate (Eur. J. Inorg. Chem. 30/2010)
Authors: Miersch Linda , Rüffer Tobias , Lang Heinrich , Schulze Steffen , Hietschold Michael , Zahn Dirk , Mehring Michael 
Published: October 2010

AbstractThe cover picture shows a novel hexanuclear bismuth oxido cluster [Bi6O4(OH)4(OTf)6], which forms a 1D coordination polymer in the solid state but is highly soluble in water and polar organic solvents. The reaction of the bismuth oxido cluster with sodium polyacrylate gave a nano‐sized hybrid material still soluble in aqueous solution. A snapshot from a molecular dynamics simulation is shown as an example of the apparent complexation of [Bi6O4(OH)4]6+ by polyacrylate in water. The synthesis and structure of [Bi6O4(OH)4(OTf)6] as well as its complexation with polyacrylate are reported in the article by D. Zahn, M. Mehring et al. on p. 4763 ff.
(R)‐(+)‐[VCD(–)984]‐4‐Ethyl‐4‐methyloctane: A Cryptochiral Hydrocarbon with a Quaternary Chiral Center. (1) Synthesis of the Enantiopure Compound and Unambiguous Determination of Absolute Configuration
Authors: Fujita Takuma , Obata Kazuhiro , Kuwahara Shunsuke , Nakahashi Atsufumi , Monde Kenji , Decatur John , Harada Nobuyuki 
Published: October 2010

AbstractEnantiopure (R)‐(+)‐[VCD(–)984]‐4‐ethyl‐4‐methyloctane (1), a cryptochiral hydrocarbon with a quaternary chiral center, was synthesized by the use of 2‐methoxy‐2‐(1‐naphthyl)propionate (MαNP) and (–)‐camphorsultam dichlorophthalic (CSDP) acid methods. The diastereomeric MαNP and CSDP acid esters prepared from racemic 2‐butyl‐2‐methyl‐1‐tetralols, were effectively separated by HPLC on silica gel, and their absolute configurations were unambiguously determined by X‐ray crystallographic analysis and 1H NMR anisotropy methods. The recovered enantiopure 2‐butyl‐2‐methyl‐1‐tetralol [(1S,2S)‐(+)‐cis‐9] was then converted into the hydrocarbon (+)‐1, the R absolute configuration of which was unambiguously determined for the first time. The structure of hydrocarbon 1 was also confirmed by NMR HSQC‐TOCSY analysis.
(R)‐(+)‐[VCD(–)984]‐4‐Ethyl‐4‐methyloctane: A Cryptochiral Hydrocarbon with a Quaternary Chiral Center. (2) Vibrational CD Spectra of Both Enantiomers and Absolute Configurational Assignment
Authors: Kuwahara Shunsuke , Obata Kazuhiro , Fujita Takuma , Miura Nobuaki , Nakahashi Atsufumi , Monde Kenji , Harada Nobuyuki 
Published: October 2010

AbstractTo characterize precisely the chiroptical properties of 4‐ethyl‐4‐methyloctane (1), one of the so‐called cryptochiral compounds, the enantiomer (S)‐(–)‐[VCD(+)984]‐1 was synthesized in an enantiopure form. The observed specific rotation values at 365 nm of both enantiomers agreed well, but were opposite in sign, confirming their enantiomeric relationship, although their absolute values were very small, reflecting their cryptochirality. The vibrational circular dichroism (VCD) spectra of both enantiomers were measured neat and showed characteristic Cotton effects around 1150–900 cm–1; the VCD spectral curves were mirror images of each other. Based on the observed positive VCD band at 984 cm–1 for (S)‐(–)‐1, its absolute configuration was fully designated as (S)‐(–)‐[VCD(+)984]‐1. The VCD spectrum was quantum chemically calculated by the DFT MO method at the B3PW91/6‐31G(d,p) level, and the simulated VCD curve for (S)‐1 agreed very well with the observed VCD spectrum of (S)‐(–)‐1, confirming the absolute configuration.
Heteroaromatic Cation‐Based Chromophores: Synthesis and Nonlinear Optical Properties of Alkynylazinium Salts
Authors: Cañeque Tatiana , Cuadro Ana M. , Alvarez‐Builla Julio , Pérez‐Moreno Javier , Clays Koen , Marcelo Gema , Mendicuti Francisco , Castaño Obis , Andrés José L. , Vaquero Juan J. 
Published: October 2010

AbstractA variety of alkynylazinium cationic (D‐π‐A+) chromophores were prepared in good yields by the reaction of bromoazinium (pyridinium, quinolinium, and isoquinolinium iodides) with alkynes under Sonogashira conditions. The analysis of the experimentally recorded spectra is supported by quantum chemical calculations using restricted configuration interaction and density functional methods. First‐order hyperpolarizabilities of (D‐π‐A+) azinium‐based cations as a new class of second‐order nonlinear optical (NLO) chromophores were also studied by hyper‐Rayleigh scattering experiments and computational procedures.
New Macrocyclic Peptidomimetics Containing 5‐Aminothiophene Subunits
Authors: Özbek Hülya , Lentz Dieter , Reissig Hans‐Ulrich 
Published: October 2010

AbstractThe synthesis of a new class of cyclic peptidomimetics containing 5‐aminothiophene subunits in their backbone is presented. A modified Gewald reaction was applied as a key step in the synthesis of the thiophene amino acid that was used as a building block in the synthesis of linear oligomers by using standard peptide coupling protocols. Macrocyclization was achieved under high dilution by using EDCI as a coupling reagent. The conformations of an acyclic dimer and a cyclic tetramer were determined by X‐ray crystallographic analyses.
Plumbagin: A New Route to the Electroanalytical Determination of Cystine
Authors: Damle Madhura S. , Newton Laura A. A. , Villalba Maria Marti , Leslie Ray , Davis James 
Published: October 2010

AbstractThe interaction between 5‐hydroxy‐1,4‐naphthoquinone and cysteine was found to give a clearly resolved voltammetric signature that could be used as an electrochemical assay for the thiol. A structure‐function study assessed the nature of the resulting voltammetric profile and the highly selective response to cysteine. The translation of the system as the basis of an assay for the determination of cystine was investigated using mercaptopropanol as the in situ reducing agent. The lack of response to the latter and high recovery performance of the label opens up a new direction for the one pot, mercury free determination of cystine.
Electrocatalytic Determination of Histamine on a Nickel‐Film Glassy Carbon Electrode
Authors: Švarc‐Gajić Jaroslava , Stojanović Zorica 
Published: October 2010

AbstractThe electrocatalysed oxidation of histamine on a thin film nickel electrode was investigated for the purpose of its chronopotentiometric determination. The oxidation mechanism implies complex and combined processes of histamine physical adsorption and consequent oxidation via joined oxidation potency of a constant current and electrochemically generated nickel‐oxihydroxide, which played a role of an electron transfer catalyst. Experimental parameters affecting the oxidation process, including type and concentration of supporting electrolyte, initial potential, oxidation current, temperature and concentration time, were optimised. Using a 240 s accumulation time, limit of detection and quantitation were 0.11 mg L−1 and 0.29 mg L−1 of histamine, respectively.