bluuebunny/crossref_metadata_2025_split huggingface dataset:
COAGULATION AND ANTICOAGULATION PARAMETERS IN MULTIPLE SCLEROSIS PATIENTS WITH AND WITHOUT COVID-19
Authors: Halenova Tetiana І. , Raksha Nataliia G. , Vovk Tetiana B. , Karbovskyy Vitalii L. , Sholomon Svitlana M. , Melnyk Volodymyr S. , Savchuk Olexii M. 
Published: May 2024

The aim. To investigate plasma levels of main coagulation and fibrinolytic factors in MS patients with and without COVID-19 history.
Materials and methods. A total of 127 participants were enrolled in this study, including 97 MS patients and 30 healthy controls (HC). Patients with MS were divided into two groups: MS+Covid group (n=41) – patients with MS, who had a laboratory-verified diagnosis of COVID-19 in the past 3-6-month period and MS group (n=56) – patients with MS, who did not suffer from COVID-19 previously. Determination of plasma levels of prothrombin, plasminogen, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), protein C (PC), soluble thrombomodulin (TM) was performed by means of enzyme-linked immunosorbent assay. Spectrophotometric techniques were used to determine concentrations of fibrinogen, soluble fibrin monomeric complexes (SFMC) as well as plasminogen activity and inhibitory potential of α-2-antiplasmin.
Results. The MS group was characterized by elevated levels of plasma prothrombin, fibrinogen, D-dimer, SFMC, soluble TM compared to HC, while PC concentration did not differ between MS and HC groups. Plasma plasminogen level as well as plasma level of the potential plasmin activity were significantly decreased in MS patients compared to HC group. The plasma tPA level was significantly reduced while plasma PAI-I level was significantly increased in MS patients compared to HC. Patients of MS group had an increased level of plasma α-2-antiplasmin activity compared with HC group. To note, most of studied parameters did not differ between two MS groups, except protein C, soluble thrombomodulin levels and plasma α-2-antiplasmin activity.
Conclusions. The results of our study showed that MS patients have got altered hemostasis parameters; however, further study is necessary to find out the relationship between particular components of coagulation and fibrinolytic systems and pathophysiology of MS. Additionally, our findings demonstrated that a SARS-CoV-2 infection had a limited effect on hemostasis parameters in MS patients, causing changes in only a few parameters, including thrombomodulin and protein C levels as well as α-2-antiplasmin activity.
The Impact of Implementing a Carbon Tax on Welfare: Case Study of Indonesia and The Other ASEAN Member Countries
Authors: Andrianus Fery , Handra Hefrizal , Ayu Putri , Safitri Pipin Dwi , Cahyadi Ria Vinola K. 
Published: May 2024

Green House Gases emitted into the atmosphere over decades cause global warming now. The aim of this research is the impact of implementing the carbon tax on welfare in Indonesia. The research method used is the CGE method using GTAP-E to evaluate energy policy in the economy. GTAP-E consists of 140 countries and 57 sectors combined into forty-two regions and eight sectors. Using carbon tax scenario (simulation 1 is 1.93 USD/ton CO 2, simulation 2 is 3.72 USD/ton CO 2, and simulation 3 is 4.83 USD/ton CO 2). The results of the Indonesian equivalent variation show a negative value. The higher the carbon tax is applied, the greater the decline in welfare. This is also felt by all research countries except the Philippines, Singapore, Thailand, Oceania, OtherSEAsia, East Asia, Argentina, Japan, Poland, Portugal, and Ukraine. The variable of the regional demand, it can be seen that the carbon tax causes Indonesia's regional income to increase, and Singapore's income to decrease, while other countries experience no change in income. The primary factor return ratio also shows that the increase in the carbon tax caused a decline in the Land, Unsklab, Sklab, Capital, and Natural Resources sectors. Indonesia's GDP also shows a decrease if a higher carbon tax is implemented, but other countries have no impact on GDP. The Carbon emissions show that it decreases to Indonesia. So, the implementation of the carbon tax causes a decrease in welfare as seen from the equivalent variation, Primary Return Ratio, and GDP in Indonesia. The government must have an alternative policy if a carbon tax is implemented in Indonesia.
Synthesis, Characterization, and Biological Activitiy of Copper (II) Complexes Containing Bidentate Schiff Bases
Authors: Mihsen Hayder Haimed 
Published: May 2024

Three ligands have been prepared by condensation of dithiooxamide with 2,4,5- trimethoxy benzaldehyde, 4- dimethylamino benzaldehyde and Vanailin. These ligands are named N,N- bis( 2,4,5 – trimethoxybenzalidene) dithiooxamide(LH1), N,N-bis(4-dimethylaminobenzalidene) dithiooxamide(LH2) and N,N-bis (vanaillidene) dithiooxamide(LH3), respectively. Complexes of LH1, LH2 and LH3 were prepared with 1:2 M:L molar ratio, with copper ion in the formulas [Cu(LH1)2(H2O)2] Cl2.H2O.0.5CH2Cl2, [Cu (LH2)2(H2O)Cl] Cl.H2O and [Cu (LH3)2(H2O)2] CL2.H2O. The ligands and their complexes have been characterized by IR, UV-Vis spectroscopy Magnetic, conductivity measurements, thermal analysis and atomic absorption technique. Biological activities of the Schiff bases and their metal complexes have been evaluated against two types of pathogenic bacteria (Escherichia coli and staphylococcus aureus ).
The Effects of Dry Needling and Physical Therapy on Improvement of Pain in Patients with Piriformis Syndrome
Authors: Dr. Ali Hussein Youssif Astokorki  
Published: May 2024

Background: Piriformis syndrome may be source of low back, buttock pain or sciatica. The purpose of this Study is to determine the effectiveness of dry needling (ND) and classical physiotherapy (PT) in the management of pain in patients with Piriformis Syndrome (PS).
Methods: A cross-sectional study was conducted 58 patients with PS were divided to two groups classical PT (n=29) and DN (n=29). 6 sessions performed for both groups, the pain measured before the first session and after each session at the end of both program, both groups were compared to determine which of them is more effective.
Results: The result showed that there was significant reduction of pain in both classical PT and dry needling groups. Paired-Sample T.Test indicated significantly difference mean ratings of pain affect from session 1 compare to session 6 for classical physical therapy T(28) = 14.065 , p = 0.000. in other group Paired-Sample T.Test also showed significantly difference mean ratings of pain affect from session 1 compare to session 6 for dry needling therapy T(28) = 11.119, p = 0.000.
Conclusion: Based on the results of statistical data analysis, it is concluded that both classical physical therapy and dry needling therapy have significant effect in treating and managing the pain in patients with piriformis syndrome and no significant difference was observed between the results of both groups.
تقنين مقياس القيم الجمالية على مدرسي ومدرسات التربية الرياضية في مدارس محافظة نينوى
Authors: Teacher.Dr. Faleh Taha Abdel Asi  , Teacher.Dr. Anmar Abdel Moneim Younis  
Published: May 2024

يهدف البحث الى تقنين مقياس القيم الجمالية المعد من قبل (البجاري ,2005) على مدرسي ومدرسات التربية الرياضية في مدارس محافظة نينوى، بعد الاجابة على التساؤلات التالية: ما دلالة معاملات الارتباط بين درجات فقرات المقياس ودرجات المجالات التي تنتمي اليها عند تطبيق المقياس على عينة البحث الحالي؟. ما دلالة معاملات الارتباط بين درجات مجالات المقياس وبين الدرجة الكلية للمقياس؟. ما مؤشرات الصدق لفقرات مقياس القيم الجمالية بطريقة المقارنة الطرفية لدى مدرسي ومدرسات التربية الرياضية؟. ما مؤشرات ثبات المقياس بعد تقنينه على عينة البحث؟ استخدم الباحثان المنهج الوصفي لملائمته وطبيعة البحث, شمل البحث عينة مكونة من (429) مدرسا ومدرسة من المتخصصين بالتربية البدنية وعلوم الرياضة والمنسوبين الى المدارس الثانوية والمتوسطة والاعدادية للمديرية العامة لتربية محافظة نينوى، استخدم في البحث مقياس القيم الجمالية المكون من (46) فقرة ، وتوصل الباحثان الى ان المقياس المقنن يمكن ان يستخدم مستقبلا لقياس مستوى القيم الجمالية لمدرسي التربية الرياضية خصوصا بعد أن أجريت عليه التعديلات ليصبح متوافقا مع الخصائص المعرفية والمهنية لأفراد العينة .
Calidad De Vida e Intervención del Trabajador Social en Atención a Personas con Tratamiento de Hemodiálisis
Authors: Mendoza-Intriago Dreysi , Moreira-Chica Tatiana 
Published: May 2024

La calidad de vida es la interacción constante entre factores económicos, sociales, las necesidades que se presentan en el transcurso de la vida y sobre todo en la incidencia individual que cada individuo tiene en el desarrollo personal, y es en ello donde el Trabajador Social cumple un rol muy imperativo, dispuesto a concentrar procesos, acciones formativas y la participación activa hacia los usuarios que lo necesita. El objetivo de la investigación fue analizar la calidad de vida e intervención del Trabajador Social en atención a personas con tratamiento de hemodiálisis en el prestador de salud RenalPRO C.A Santa Elena. Para el diseño de la investigación se estableció el uso del paradigma mixto, el cual focalizo la aplicabilidad de instrumentos cuantitativos y cualitativos, bajo el procesamiento de datos estadísticos y el desarrollo de evaluación mediante el Alfa de Cronbach que midió la fiabilidad de la calidad de vida. Los resultados indican que las personas tienen una calidad de vida normal, es decir que ejecutan sus actividades normales, y que están dispuestas a seguir avanzando a pesar de las situaciones que se generan en el entorno de diálisis. De acuerdo con la efectividad de resultados se trabajó con una confiabilidad del instrumento del 0,982, de lo cual su margen de error fue mínimo, en esto recaen que los resultados expresados fueron compilados y comprendidos para dar énfasis sobre la calidad de vida, en el caso del personal de Trabajo Social se pudo reconocer que existe el dominio y la comprensión sobre los temas que están relacionados en pacientes con este tratamiento, y que su principal rol está en el direccionamiento y atención al bienestar el paciente.
Clinical significance of procollagen type III N-terminal peptide in patients with alcoholic liver disease
Authors: Geyvandova N. I. , Bolbat G. K. , Yagoda A. V. 
Published: May 2024

Background. Alcoholic liver disease (ALD) – is a disease that leads to the development of liver cirrhosis (LC) with a high mortality rate. N-terminal type lll procollagen peptide (PIIINP) is one of the optimal biomarkers for assessing fibrogenesis.Objective: to determine the clinical significance of PIIINP blood level in patients with ALD.Materials and methods. 97 patients with ALD were examined. The age of the patients was 48,5±9,9 years, there were 30 women, 67 men. Steatosis was diagnosed in 12 patients, 11 – alcoholic hepatitis (AH), 74 – LC. In group with LC, 16 patients was diagnosed AH against confirmed cirrhosis. PIIINP blood level determined by ELISA. Control group consisted of 22 healthy volunteers who have not consumed alcohol in hepatotoxic doses.Results. In all patients, PIIINP blood level was increased. In steatosis PIIINP slightly increased the norm, indicating the beginning of fibrogenesis. In LC, PIIINP blood level was higher than in patients with steatosis, which reflected increasing of fibrosis and progression of the disease. The highest levels of PIIINP were observed in cases with AH. Levels PIIINP in patients with AH but without LC and in patients with AH against the background of the formed LC did not differ. In Maddray index of more than 32 (9 patients), the PIIINP level was higher than in 18 patients with index values <32, which confirmed the role of AH in development of fibrosis and decompensation of liver function.Conclusion. Determination of PIIINP blood level in patients with ALD will allow predict the activity of fibrogenesis and the severity of subsequent changes in liver tissue. In cases of severe AH, PIIINP may be an additional criterion determining the severity and prognosis of hepatitis outcomes.
Pharmacogenomic analysis in adrenocortical carcinoma reveals genetic features associated with mitotane sensitivity and potential therapeutics
Authors: Zhang Jie , Wu Luming , Su Tingwei , Liu Haoyu , Jiang Lei , Jiang Yiran , Wu Zhiyuan , Chen Lu , Li Haorong , Zheng Jie , Sun Yingkai , Peng Hangya , Han Rulai , Ning Guang , Ye Lei , Wang Weiqing 
Published: May 2024

BackgroundAdrenocortical carcinoma (ACC) is an aggressive endocrine malignancy with limited therapeutic options. Treating advanced ACC with mitotane, the cornerstone therapy, remains challenging, thus underscoring the significance to predict mitotane response prior to treatment and seek other effective therapeutic strategies.ObjectiveWe aimed to determine the efficacy of mitotane via an in vitro assay using patient-derived ACC cells (PDCs), identify molecular biomarkers associated with mitotane response and preliminarily explore potential agents for ACC.MethodsIn vitro mitotane sensitivity testing was performed in 17 PDCs and high-throughput screening against 40 compounds was conducted in 8 PDCs. Genetic features were evaluated in 9 samples using exomic and transcriptomic sequencing.ResultsPDCs exhibited variable sensitivity to mitotane treatment. The median cell viability inhibition rate was 48.4% (IQR: 39.3-59.3%) and -1.2% (IQR: -26.4-22.1%) in responders (n=8) and non-responders (n=9), respectively. Median IC50 and AUC were remarkably lower in responders (IC50: 53.4 µM vs 74.7 µM, P&lt;0.0001; AUC: 158.0 vs 213.5, P&lt;0.0001). Genomic analysis revealed CTNNB1 somatic alterations were only found in responders (3/5) while ZNRF3 alterations only in non-responders (3/4). Transcriptomic profiling found pathways associated with lipid metabolism were upregulated in responder tumors whilst CYP27A1 and ABCA1 expression were positively correlated to in vitro mitotane sensitivity. Furthermore, pharmacologic analysis identified that compounds including disulfiram, niclosamide and bortezomib exhibited efficacy against PDCs.ConclusionACC PDCs could be useful for testing drug response, drug repurposing and guiding personalized therapies. Our results suggested response to mitotane might be associated with the dependency on lipid metabolism. CYP27A1 and ABCA1 expression could be predictive markers for mitotane response, and disulfiram, niclosamide and bortezomib could be potential therapeutics, both warranting further investigation.
HIV immunological non-responders are characterized by extensive immunosenescence and impaired lymphocyte cytokine production capacity
Authors: Vos Wilhelm A. J. W. , Navas Adriana , Meeder Elise M. G. , Blaauw Marc J. T. , Groenendijk Albert L. , van Eekeren Louise E. , Otten Twan , Vadaq Nadira , Matzaraki Vasiliki , van Cranenbroek Bram , Brinkman Kees , van Lunzen Jan , Joosten Leo A. B. , Netea Mihai G. , Blok Willem L. , van der Ven Andre J. A. M. , Koenen Hans J. P. M. , Stalenhoef Janneke E. 
Published: May 2024

IntroductionImmunological non-responders (INR) are people living with HIV (PLHIV) who fail to fully restore CD4+ T-cell counts despite complete viral suppression with antiretroviral therapy (ART). INR are at higher risk for non-HIV related morbidity and mortality. Previous research suggest persistent qualitative defects.MethodsThe 2000HIV study (clinical trials NTC03994835) enrolled 1895 PLHIV, divided in a discovery and validation cohort. PLHIV with CD4 T-cell count &lt;350 cells/mm3 after ≥2 years of suppressive ART were defined as INR and were compared to immunological responders (IR) with CD4 T-cell count &gt;500 cells/mm3. Logistic and rank based regression were used to analyze clinical data, extensive innate and adaptive immunophenotyping, and ex vivo monocyte and lymphocyte cytokine production after stimulation with various stimuli.ResultsThe discovery cohort consisted of 62 INR and 1224 IR, the validation cohort of 26 INR and 243 IR. INR were older, had more advanced HIV disease before starting ART and had more frequently a history of non-AIDS related malignancy. INR had lower absolute CD4+ T-cell numbers in all subsets. Activated (HLA-DR+, CD38+) and exhausted (PD1+) subpopulations were proportionally increased in CD4 T-cells. Monocyte and granulocyte immunophenotypes were comparable. INR lymphocytes produced less IL-22, IFN-γ, IL-10 and IL-17 to stimuli. In contrast, monocyte cytokine production did not differ. The proportions of CD4+CD38+HLA-DR+ and CD4+PD1+ subpopulations showed an inversed correlation to lymphocyte cytokine production.ConclusionsINR compared to IR have hyperactivated and exhausted CD4+ T-cells in combination with lymphocyte functional impairment, while innate immune responses were comparable. Our data provide a rationale to consider the use of anti-PD1 therapy in INR.
Plasminogen activator inhibitor-1 promotes immune evasion in tumors by facilitating the expression of programmed cell death-ligand 1
Authors: Ibrahim Abd Aziz , Fujimura Taku , Uno Tomoko , Terada Tomoya , Hirano Ken-ichi , Hosokawa Hiroyuki , Ohta Akio , Miyata Toshio , Ando Kiyoshi , Yahata Takashi 
Published: May 2024

BackgroundIncreased levels of plasminogen activator inhibitor-1 (PAI-1) in tumors have been found to correlate with poor clinical outcomes in patients with cancer. Although abundant data support the involvement of PAI-1 in cancer progression, whether PAI-1 contributes to tumor immune surveillance remains unclear. The purposes of this study are to determine whether PAI-1 regulates the expression of immune checkpoint molecules to suppresses the immune response to cancer and demonstrate the potential of PAI-1 inhibition for cancer therapy.MethodsThe effects of PAI-1 on the expression of the immune checkpoint molecule programmed cell death ligand 1 (PD-L1) were investigated in several human and murine tumor cell lines. In addition, we generated tumor-bearing mice and evaluated the effects of a PAI-1 inhibitor on tumor progression or on the tumor infiltration of cells involved in tumor immunity either alone or in combination with immune checkpoint inhibitors.ResultsPAI-1 induces PD-L1 expression through the JAK/STAT signaling pathway in several types of tumor cells and surrounding cells. Blockade of PAI-1 impedes PD-L1 induction in tumor cells, significantly reducing the abundance of immunosuppressive cells at the tumor site and increasing cytotoxic T-cell infiltration, ultimately leading to tumor regression. The anti-tumor effect elicited by the PAI-1 inhibitor is abolished in immunodeficient mice, suggesting that PAI-1 blockade induces tumor regression by stimulating the immune system. Moreover, combining a PAI-1 inhibitor with an immune checkpoint inhibitor significantly increases tumor regression.ConclusionsPAI-1 protects tumors from immune surveillance by increasing PD-L1 expression; hence, therapeutic PAI-1 blockade may prove valuable in treating malignant tumors.
The single-dose Janssen Ad26.COV2.S COVID-19 vaccine elicited robust and persistent anti-spike IgG antibody responses in a 12-month Ugandan cohort
Authors: Serwanga Jennifer , Kato Laban , Oluka Gerald Kevin , Ankunda Violet , Sembera Jackson , Baine Claire , Kitabye Isaac , Namuyanja Angela , Opio Solomon , Katende Joseph Ssebwana , Ejou Peter ,   The COVID-19 Immunoprofiling Team, Kaleebu Pontiano 
Published: May 2024

IntroductionThe study investigation examined the immune response to the Janssen Ad26.COV2.S COVID-19 vaccine within a Ugandan cohort, specifically targeting antibodies directed against spike (S) and nucleocapsid (N) proteins. We aimed to examine the durability and robustness of the induced antibody response while also assessing occurrences of breakthrough infections and previous anti-Spike seropositivity to SARS-CoV-2.MethodsThe study included 319 specimens collected over 12 months from 60 vaccinees aged 18 to 64. Binding antibodies were quantified using a validated ELISA method to measure SARS-CoV-2-specific IgG, IgM, and IgA levels against the S and N proteins.ResultsThe results showed that baseline seropositivity for S-IgG was high at 67%, increasing to 98% by day 14 and consistently stayed above 95% for up to 12 months. However, S-IgM responses remained suboptimal. A raised S-IgA seropositivity rate was seen that doubled from 40% at baseline to 86% just two weeks following the initial vaccine dose, indicating sustained and robust peripheral immunity. An increase in N-IgG levels at nine months post-vaccination suggested breakthrough infections in eight cases. Baseline cross-reactivity influenced spike-directed antibody responses, with individuals harbouring S-IgG antibodies showing notably higher responses.DiscussionRobust and long lasting vaccine and infection-induced immune responses were observed, with significant implications for regions where administering subsequent doses poses logistical challenges.
Bacterial enrichment prior to third-generation metagenomic sequencing improves detection of BRD pathogens and genetic determinants of antimicrobial resistance in feedlot cattle
Authors: Herman Emily K. , Lacoste Stacey R. , Freeman Claire N. , Otto Simon J. G. , McCarthy E. Luke , Links Matthew G. , Stothard Paul , Waldner Cheryl L. 
Published: May 2024

IntroductionBovine respiratory disease (BRD) is one of the most important animal health problems in the beef industry. While bacterial culture and antimicrobial susceptibility testing have been used for diagnostic testing, the common practice of examining one isolate per species does not fully reflect the bacterial population in the sample. In contrast, a recent study with metagenomic sequencing of nasal swabs from feedlot cattle is promising in terms of bacterial pathogen identification and detection of antimicrobial resistance genes (ARGs). However, the sensitivity of metagenomic sequencing was impeded by the high proportion of host biomass in the nasal swab samples.MethodsThis pilot study employed a non-selective bacterial enrichment step before nucleic acid extraction to increase the relative proportion of bacterial DNA for sequencing.ResultsNon-selective bacterial enrichment increased the proportion of bacteria relative to host sequence data, allowing increased detection of BRD pathogens compared with unenriched samples. This process also allowed for enhanced detection of ARGs with species-level resolution, including detection of ARGs for bacterial species of interest that were not targeted for culture and susceptibility testing. The long-read sequencing approach enabled ARG detection on individual bacterial reads without the need for assembly. Metagenomics following non-selective bacterial enrichment resulted in substantial agreement for four of six comparisons with culture for respiratory bacteria and substantial or better correlation with qPCR. Comparison between isolate susceptibility results and detection of ARGs was best for macrolide ARGs in Mannheimia haemolytica reads but was also substantial for sulfonamide ARGs within M. haemolytica and Pasteurella multocida reads and tetracycline ARGs in Histophilus somni reads.DiscussionBy increasing the proportion of bacterial DNA relative to host DNA through non-selective enrichment, we demonstrated a corresponding increase in the proportion of sequencing data identifying BRD-associated pathogens and ARGs in deep nasopharyngeal swabs from feedlot cattle using long-read metagenomic sequencing. This method shows promise as a detection strategy for BRD pathogens and ARGs and strikes a balance between processing time, input costs, and generation of on-target data. This approach could serve as a valuable tool to inform antimicrobial management for BRD and support antimicrobial stewardship.