bluuebunny/crossref_metadata_2025_split huggingface dataset:
Congestion in chronic systolic heart failure is related to renal dysfunction and increased mortality
Authors: Damman Kevin , Voors Adriaan A. , Hillege Hans L. , Navis Gerjan , Lechat Philippe , van Veldhuisen Dirk J. , Dargie Henry J. ,   for the CIBIS‐2 Investigators and Committees
Published: August 2010

AimsTo investigate the relationship between signs and symptoms of congestion, renal impairment and outcome in chronic heart failure (CHF) patients.Methods and resultsThe Cardiac Insufficiency Bisoprolol Study II included 2647 NYHA class III/IV CHF patients. Congestion was estimated by the presence of elevated jugular venous pressure, orthopnoea, ascites, or oedema. Glomerular filtration rate (eGFR) was estimated using the simplified modification of diet in renal disease (sMDRD) formula. The main outcome consisted of all‐cause mortality. Mean age was 61 ± 11 years, 80% of patients were male, and mean eGFR was 77 ± 31 mL/min/1.73 m2. A total of 1234 (47%) patients had at least one sign, and 10% of patients had more than three signs of congestion. Mean eGFR was lower in patients with signs of congestion (75 ± 30 vs. 79 ± 31 mL/min/1.73 m2, P < 0.001). In multivariable analysis, signs of congestion remained independently related to eGFR (P = 0.012). Mortality rates almost doubled from no signs to three or more signs of congestion (11 vs. 20%, respectively, P < 0.0001). Patients with any signs of congestion also had a worse outcome. Adjusted hazard ratio was 1.42 (1.14–1.77), P < 0.01 for mortality and 1.21 (1.05–1.39), P < 0.01 for the composite endpoint.ConclusionSigns and symptoms of congestion are associated with renal impairment and are independent determinants of prognosis in patients with CHF.
Sciatic nerve block for children
Authors: McNICOL L. R. 
Published: August 2010

SummaryThis study aimed to establish whether it was practical to perform sciatic nerve block by the anterior approach in a group of children of different ages and weights. A total of 82 blocks were performed of which 78 (95.2%) were judged to have been successful. The technique of the block differed slightly from that used in adult practice, in that great reliance was placed on the loss of resistance felt as the needle point passed through the thigh muscles into the sciatic neurovascular compartment. There were no immediate or late complications associated with this block in any of the patients. It is concluded that the block is easy to perform and can produce reliable postoperative analgesia for most common operations on the foot and ankle in paediatric practice.
Serum alkaline phosphatase inheritance in the pigeon1
Authors: Brown Russell V. , Manley James H. 
Published: August 2010

SummarySerum alkaline phosphatase inheritance was studied in 126 racing homer pigeons {Columba livid). Discontinuous polyacrylamide electrophoresis was used. Gels were stained with Blue RR salts with alphanaphthyl phosphate as a substrate. Seven pheno‐types were found. Inheritance was postulated to be by four codominant alleles. Gene frequencies were: q.ApA, 0.284; qApB, 0.602; qApC, 0.45; and qApD, 0.68.
Haemolytic factor in bovine seminal vesicle fluid
Authors: Matoušek J. , Staněk R. 
Published: August 2010

SummaryThe haemolytic factor of bovine seminal fluid has a protein nature; it is present in the precipitate obtained by treating the fluid with alcohol‐ether, ammonium sulphate and rivanol; it occurs in three out of five protein fractions obtained by chromatographic separation on Sephadex G 100.of Enzymatic treatment of seminal vesicle fluid by pronase caused total inactivation of the haemolytic factor. Chymotrypsin caused considerable damage, while a number other enzymes did not affect the activity of the haemolytic factor.
Glutathione and transferrin in rhesus monkeys1
Authors: Brown Russell V. , Goodman Morris , Gavan James A. 
Published: August 2010

SummaryTransferrin types and glutathione (GSH) levels were determined for 38 rhesus monkeys (Macaca mulatto). Eight transferrin alleles were found: their gene frequencies were: TfA 0.04, Tfc 0.50, TfD 0.03, TfD 0.09, TF 0.11, TF 0.05, TfG 0.11, and Tf H' 0.08. The 16 phenotypes found and the number of each was: Tf AC 3, Tf CC 9, Tf CD‘ 2, Tf CD 2, Tf CF 5, Tf CF 3, Tf CH’ 3, Tf CG 2, Tf D'D‘ 1, Tf D'F 1, Tf D'G 1, TfD'H’ l, TfFG2, TfF'G ‘l.TfCG l.and Tf H'H’ 1.Mean GSH level was 310.6 mg/100 ml red cells. The Tf CC group had significantly lower GSH levels than Tfc heterozygotes or non‐Tfc homozygotes. The Tfc heterozygotes had significantly lower GSH levels than non‐Tfc homozygotes. Tfc, TfD' and TF alleles were associated with lower GSH levels. Glutathione levels were higher in older animals and were higher in September than in December. Sex differences in glutathione levels were not found.
Haemoglobin variants in cattle
Authors: Braend M. 
Published: August 2010

SummaryFrom a comparative survey of reports on cattle Hb variants it is concluded that prior to the present communication the occurrence of at least seven different adult Hb molecules have been reported.In addition comes the finding of a new Hb variant called HbG which is reported in this study. This was found in three animals among 101 East African Zebu cattle. It migrated more slowly than any cattle Hb variant previously reported and made up 20% of total Hb.The gene frequencies of the East African Zebu population were: HbA= 0.52, HbB= 0.32, Hbc= 0.14 and HbG= 0.01.
Glucose‐6‐?hosphate dehydrogenase deficiency, haptoglobin and hemoglobin variants in dogs
Authors: Naik S. N. , Anderson D. E. , Jardine J. H. , Clifford D. H. 
Published: August 2010

SummaryBlood samples from 31 male and 34 female, adult, healthy dogs of different breeds were studied for erythrocytic G‐6‐PD activity. The hemolysates were also studied electrophoretically for G‐6‐PD, 6‐PGD, and hemoglobin variants. Most of the plasma samples revealed a human Hp 1–1 type of band while three samples had an additional fast‐moving band that disappeared on addition of an excess of hemoglobin and two samples were ahaptoglobinemic. G‐6‐PD deficiency was detected in eight samples, and it was more frequent in males than in females. The implications of G‐6‐PD deficiency with no difference in the electrophoretic pattern and of ahaptoglobinemia are discussed with respect to different genetic and clinical possibilities.
A third allele of serum esterases in domestic fowl and the strain distribution of six phenotypes*
Authors: Grunder A. A. 
Published: August 2010

SummaryA starch gel electrophoretic technique has been described which utilized a pH 8.9 Tris‐citrate gel buffer and facilitated the resolution of a new serum esterase zone of fowl. This zone was shown to be controlled by allele EsC, the third in the series EsA, EsB, and EsC.Allelic frequencies were determined in five egg‐type strains and seven meat‐type strains. EsA was predominant in egg‐type birds while EsB was the most frequent allele in meat‐type birds.
Polymorphism of canine serum albumin
Authors: Day Mae E. , Kraay G. J. , Stevens R. W. C. 
Published: August 2010

SummaryThree serum albumin phenotypes were observed in a population of 257 dogs consisting of 18 breeds and breed crosses. Canine serum albumins are controlled by an autosomal locus with two codominant alleles AlbF and AlbS. Separation of the albumin types was possible by using buffer solutions with a pH of about 4.
The agglutination of cattle erythrocytes by plant extracts
Authors: Hines H. C. 
Published: August 2010

SummarySeven of ten species of seed extracts which were tested exhibited differential agglutination of cattle cells. Repeatability studies indicated no differences in cells collected at different times or washed the previous day. Pronounced animal effects and storage time effects were detected, however. In an investigation of 1727 animals strong relationships were detected between six lectins and the V specificity. Other significant relationships may exist between certain lectin specificities in the Y1 and I antigens. The lectin reaction patterns were also shown to be highly related to each other. Breed frequencies for the lectin detected specificities were determined.
Inheritance of esterases in the white crappie (Pomoxis annularis), black crappie (P. nigromaculatus), and their F1 and F2 interspecific hybrids*
Authors: Metcalf R. A. , Whitt G. S. , Childers W. F. 
Published: August 2010

SummaryWhite crappie, Pomoxis annularis, and black crappie, P. nigromaculatus, were hybridized to produce Fi and F2 generations. There was considerable tissue specific esterase gene function within each parental species. Many of the esterases were homologous but possessed different electrophoretic mobilities between the two species which permitted their use as gene markers. The pattern of inheritance and expression of a number of esterase genes was determined in the Fi and F2 interspecific hybrids.Although certain key morphological phenotypes characteristic of the black crappie predominate in the Fi hybrid, the enzyme phenotypes reflect the contributions of both co‐dominant parental genomes. There is a higher percentage of heterozygotes than expected in the F2 generation which may be indicative of heterosis. The genetic analysis also provided a better understanding of the polypeptide composition of these esterases.
Molecular genetics of avian proteins. XL Egg proteins of Gallus gallus, G. sonnerati and hybrids
Authors: Baker C. M. Ann , Manweil Clyde 
Published: August 2010

SummaryBy a variety of electrophoretic procedures it has been found that Gallus gallus, whether as jungle fowl or as chickens, differs consistently from Sonnerat's jungle fowl, G. sonnerati, in three egg white proteins: G3 ovoglobulin, G2 ovoglobulin and glutamyl peptidase. The electrophoretic patterns of egg white hybrids between G. gallus and G. sonnerati are identical to those obtained after simpiy mixing the egg whites; there is no evidence for hybrid protein zones. Although Japanese data on serum amylase can be interpreted as indicating introgressive hybridization in the origin of the domestic fowl, so far the egg protein data are negative in this respect.
Soluble glutamic oxalacetic transaminase (GOT) polymorphism in cattle
Authors: Ansay M. , Hanset R. 
Published: August 2010

SummaryZymograms performed on extracts of bovine white cells revealed two phenotypes in the soluble (anodal) isozyme of glutamic oxalacetic transaminase (GOT). Genetic data are consistent with the hypothesis that two co‐dominant, autosomal alleles, GOT‐1‐A and GOT‐1‐B, give rise to the phenotypes GOT‐1‐AA, GOT‐1‐AB and GOT‐1‐BB. The phenotype BB has not been encountered. The allele GOT‐1‐B has been observed in one of the five breeds surveyed, the Central and Upper Belgium breed where it has a frequency of about 0,008.
Distribution of R‐r‐i‐blood group system in Australian sheep
Authors: Healy Peter J. 
Published: August 2010

SummaryThe distribution of the R‐r‐i blood group system was studied in eight breeds of Australian sheep. The recessive i gene was found to be absent from Merinos and, consequently, the i phenotype was absent in Merino crosses. Distribution of the groups in Dorset Horns and Ryelands was found to be strikingly different from that of other breeds. Two animals were found to have anti‐r antibodies in serum.
Studies on the transferrins of goats. 3. Evidence for a third transferrin allele
Authors: Watanabe Seiki , Suzuki Shozo 
Published: August 2010

SummaryA new allele Tfc in serum transferrin of goats is postulated. It was considered that serum transferrins in goats classified into six phenotypes are genetically controlled by three codominant alleles, TfA, TfB and Tfc. Frequencies of Tfc were low in native goats in Korea, Philipines and Thailand, and this allele is yet to be observed in other breeds of goats.
En, Eo ‐ further new factors in the complex E blood group system of the pig
Authors: Hojný J. , Hradecký J. 
Published: August 2010

SummaryTwo new erythrocyte antigens have been detected by isoimmune antibodies obtained from Gottingen Miniature pigs. By family studies they proved to belong to the E system. The factor designated En is genetically controlled by alleles E2=Eedghkmn, E3=Eaegln, E4=Eedfhkmn, E6=Eaefln, E9=Eedghjmn, E12=Eaegmno and Eedgkln, and the factor designated Eo by the allele E12=Eaegmno.The En blood factor occurs in all the breeds studied and in frequency it comes close to a related Ee factor. It stands in opposition not only to Eb but also to Ei factor. The Eo factor can be characterized as a rarely occurring Ea subgroup. Besides being antithetic to Ed it is also antithetic to El and Ei factors. For the present, it was found only in Miniature pigs.
An additional locus controlling liver esterases of chickens
Authors: Okada Ikuo 
Published: August 2010

SummaryStarch gel electrophoresis of liver extracts of chickens revealed six regions, designated I to VI, of esterase activity. Three phenotypes, A, B and AB, were found in esterases in region I. These phenotypes were shown to be controlled by a pair of codominant autosomal alleles, Es‐5A and Es‐5B.
Further contribution to the H blood group system in pigs
Authors: Hojný J. 
Published: August 2010

SummaryThe haemolytic reagent allowing direct serological detection of He homozygous pigs was obtained by the immunization of a Landrace sow. Another monospecific reagent prepared from immune serum of a Miniature pig made possible the evidence for a new factor of the H system ‐ He. This factor is genetically controlled by the allele Hbe. Its frequency in Miniature pigs was 0.099.The H system with alleles H1=Ha, H2= Hb, H3= Hab, H4=Hcd, H5= Hbd, H6=Hbe and H7= H‐ continues to be a genetically open system.
The genetics of an electrophoretic variant of an erythrocytic protein in the house mouse (Mus musculus)
Authors: Kirby G. C. 
Published: August 2010

SummaryAn electrophoretic mobility variant of a protein band anodal to haemoglobin in alkaline starch gels was found in wild house mouse populations in South Australia. Most wild mice and all inbred lines examined are homozygous for the Erp‐1a allele at the locus controlling this variation. The rare allele Erp‐1b, which has a frequency of about 1% in South Australia, produces a protein band of slower mobility in alkaline gels. Homozygotes show one protein band whilst the heterozygote has three bands. The Erp‐1 protein does not appear to be haemoglobin or carbonic anhydrase. The Erp‐1 locus is closely linked to Es‐1 on chromosome 8 with a recombination fraction of 6.04 ± 1.32%.
The effect of sheep anti‐L and certain cattle S‐system reagents on active potassium transport in sheep and cattle red blood cells
Authors: Ellory J. C. , Tucker Elizabeth M. , Rasmusen B. A. 
Published: August 2010

SummaryCertain anti‐sheep L antisera stimulated active potassium transport in cattle red cells. All cattle red cells tested (red cells from 21 Jersey cows) which had an internal K level of less than 70 mmol/1 were stimulated but those with more than 75 mmol/1 of K (red cells from 7 Jersey cows) were not stimulated. Cattle S‐system reagents and isoimmune cattle sera produced by injecting red cells of low‐potassium type into cows with cells of high‐potassium type failed to stimulate active potassium transport in either cattle or sheep red cells.
Study on the variation of precipitin reactions in normal sera of chickens
Authors: Petrovský E. , Reyes M. , Carbonell G. , García B. 
Published: August 2010

SummaryBreed distribution of precipitin reactants (of types A and B) was encountered in: Brahma and Columbian Plymouth Rock ‐ high strength and frequencies; White Cornish, Dark Cornish, Minorca, Barred Plymouth Rock, New Hampshire, Rhode Island Red, Catalana and Cubalaya ‐ low to medium strength and frequencies; Australorp, White Leghorn, Polish, Sexline ‐ no reactions. Cocks exhibited only type A reactants. Both A and B substances were detected in egg yolks. Type A substances were encountered in sera of newly hatched chicks. Their decay was slow ‐ the mean persistence time being about 15 days. Only two young chicks among 160 tested exhibited the reactants of type B. Hormonal treatments of White Leghorn and Rhode Island Red cocks with testosterone propionate, progesterone, oestradiol benzoate or oestradiol benzoate along with progesterone failed to induce the synthesis of precipitin reactants.
Biochemical polymorphism of starry flounder, Platichthys stellatus, from the northwestern and northeastern Pacific Ocean
Authors: Johnson Allyn G. , Beardsley Alan J. 
Published: August 2010

SummaryThree enzyme systems, MDH, PGM and AGPDH, were investigated in starry flounder collected from off northwestern Hokkaido Island, Japan; western Kamchatka; USSR; Puget Sound, Washington, USA; and Yaquina Bay, Oregon, USA. Significant differences in MDH allele distribution were found between the Asian‐Puget Sound and Yaquina Bay‐Puget Sound samples suggesting that this system may be useful to separate different population units.The allele frequencies of MDH and PGM did not show significant variation with body length and handedness in starry flounder collected from Puget Sound, Washington, and did not support the hypothesis of differential viability between dextral and sinistrial forms of starry flounder.
Alterations in reactivity of the blood factors of cattle red cells after pronase treatment
Authors: Sellei J. 
Published: August 2010

SummaryPronase treatment of cattle red cells produced various effects: (a) an increase in reactivity of the J factor and evolution of a specific cryptoantigen; (b) decrease in the A‐B, G2, K, I2 O2, O3, P, Q, T1; Y2, A, B', E'2, E'3, I', K', O'‐L'‐V‐L and M, factors, but (c) no change in the specifity or in the titre of the remaining 16 different blood factors. Most of the pronase‐affectable blood factors were destroyed in a rather narrow but characteristic range of pronase treatment intensities. However, at like intensities, variations were demonstrable due to the fact that the blood factor occurred (a) in red cells from different individuals, and (b) in different phenogroups or subgroups of the B locus.
Population differences of aspartate aminotransferase and peptidase in the bay mussel Mytilus edulis
Authors: Johnson Allyn G. , Utter Fred M. 
Published: August 2010

SummaryThis investigation has demonstrated considerable heterogeneity among populations and some heterogeneity within populations in the distribution of alleles at two variant loci of Mytilus edulis. Although the causes of this variation remain obscure, some speculations have been made on the basis of available data. A cline for aspartate aminotransferase (AAT) alleles has been observed on the Pacific Coast. An immigration model has been proposed to explain the atypical ecological and genetic characteristics of large mussels found on Amchitka Island, Alaska. Marked differences were found in the distribution of peptidase alleles among collections from Southern California, the North Pacific Ocean, and New Jersey. Deviations from random distribution of phenotypes observed in comparisons made between large and small mussels from the New Jersey collection may reflect selection operating on these loci in this population.
Isoimmune anti‐A blood group reagents in pigs
Authors: Hojný J. , Hradecký J. , Jílek F. 
Published: August 2010

SummaryImmunization or reimmunization of A‐negative pigs with red blood cells (RBC) from A‐positive donors yielded anti‐A antibodies reacting in high titres with pheno‐type A(Ac) RBC and, in some cases, in low dilutions, with phenotype Aw(Ap) RBC also. An attempt to raise the anti‐A level by immunization with saliva which contained A substance was likewise successful.Repeated immunization of A‐negative recipients with the RBC of A‐positive donors (compatible in all other factors), with the aid of adjuvant, is recommended as the best way of obtaining Aw typing reagents.
Association between αs1‐, β‐ and x‐casein loci in two Italian cattle breeds
Authors: Voglino G. F. , Carignano I. 
Published: August 2010

SummaryThe genetic polymorphism αs1‐, β‐ and x‐caseins was examined by gel electrophoresis in two Italian breeds, Valdostana and Piedmont.The results obtained from acid and basic migration show that the gene frequencies of the two breeds are very similar.Non independent assortment of genotypes among these milk protein loci was also studied.Results of analyses carried out on loci pairs showed that the genetic complex αs1‐CnB‐β‐CnA2 was the most common in both breeds. In addition, the measure of linkage disequilibrium or gametic association (denoted A) showed a close association between αs1‐Cn and β‐Cn, and between β‐Cn. and x‐Cn. No significant association was found between αs1‐Cn and x‐Cn. This is in line with the model proposed by Grosclaude et al. (1973).
Electrophoretic variation in intertidal and subtidal organisms in Puget Sound, Washington
Authors: Johnson Allyn G. , Utter Fred M. 
Published: August 2010

SummaryThis paper presents information on the biochemical properties (via starch gel electrophoresis) of 26 marine species (15 fish and 11 invertebrates). Evidence is presented on 48 polymorphisms found in the 26 species. Estimates of the proportion of the genome heterozygous per individual per species were calculated and ranged from 0.000 to 0.176 for the invertebrates and from 0.000 to 0.074 for the fish species. Estimates of the percentage of loci polymorphic per species for invertebrates were 0 to 54 %; for the fish species, the range was 0 to 20 %.
J blood group active lipoproteins extracted from bovine erythrocytes
Authors: Kirchhof Bruno , Thiele Otto Wolfgang , Petri Maria Elisabeth , Koch Johannes 
Published: August 2010

SummaryGhosts of J‐positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion‐free water, and then extracted with a water‐n‐pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood‐group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high‐density lipoprotein fraction, while the low‐density lipoprotein fraction contained 1/3 J activity. This result is consistent with our finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.
A transferrin variant Tf I in crosses of the wild and domestic pigs
Authors: Glasnak V. , Stratil A. , Schleger W. 
Published: August 2010

SummaryIn crosses of the wild pig (Sus scrofa attila Thomas) with the domestic pig a transferrin variant, Tf I, was detected, electrophoretic mobility of which was slightly faster than the mobility of the variant Tf A. From the results of starch gel electrophoresis, isolation, neuraminidase treatment, autoradiography, and genetic analysis of several families, it can be concluded that the Tf I variant is genetically controlled by the allele Tf1. Thus the number of alleles in the transferrin system of the pig has increased to six (TfI, TfATfB, TfC, TPD and TfE).
New protein polymorphisms in cattle
Authors: Thinnes F. , Geldermann H. , Wens U. 
Published: August 2010

SummaryPreparation techniques are outlined for blood samples from cattle, which permit rapid separation of several constant fractions within a large number of animals. Electrophoretical examination of the blood fractions, the methods for which are described, rendered possible the phenotyping of 5 new polymorphic protein systems. Furthermore the electrophoresis of erythrocyte carboanhydrase was tested.In about 550 cattle, including familial material of the breed ‘Deutsche Schwarz‐bunte’, the observed phenotypes, segregation data and allele frequencies of 7 polymorphisms, are described. We were able to find new polymorphisms of serum post‐transferrins (Ptf), leucocytic proteins 1 and 2 (Leu1 and Leu2), and erythrocytic proteins 1 and 2 (Ery1, and Ery2). Moreover, data of carbonic anhydrase isoenzymes stained with protein dyes, as well as identified as esterases, are given.