bluuebunny/crossref_metadata_2025_split huggingface dataset:
Initial CD34+ cell‐enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon Ex vivo expansion
Authors: Andrade Pedro Z. , da Silva Cláudia Lobato , dos Santos Francisco , Almeida‐Porada Graça , Cabral Joaquim M.S. 
Published: March 2011

AbstractSince umbilical cord blood (UCB), contains a limited hematopoietic stem/progenitor cells (HSC) number, successful expansion protocols are needed to overcome the hurdles associated with inadequate numbers of HSC collected for transplantation. UCB cultures were performed using a human stromal‐based serum‐free culture system to evaluate the effect of different initial CD34+ cell enrichments (Low: 24 ± 1.8%, Medium: 46 ± 2.6%, and High: 91 ± 1.5%) on the culture dynamics and outcome of HSC expansion. By combining PKH tracking dye with CD34+ and CD34+CD90+ expression, we have identified early activation of CD34 expression on CD34− cells in Low and Medium conditions, prior to cell division (35 ± 4.7% and 55 ± 4.1% CD34+ cells at day 1, respectively), affecting proliferation/cell cycle status and ultimately determining CD34+/CD34+CD90+ cell yield (High: 14 ± 1.0/3.5 ± 1.4‐fold; Medium:22 ± 2.0/3.4 ± 1,0‐fold; Low:31 ± 3.0/4.4 ± 1.5‐fold) after a 7‐day expansion. Considering the potential benefits of using expanded UCB HSC in transplantation, here we quantified in single UCB units, the impact of using one/two immunomagnetic sorting cycles (corresponding to Medium and High initial progenitor content), and the average CD34+ cell recovery for each strategy, on overall CD34+ cell expansion. The higher cell recovery upon one sorting cycle lead to higher CD34+ cell numbers after 7 days of expansion (30 ± 2.0 vs. 13 ± 1.0 × 106 cells). In particular, a high (>90%) initial progenitor content was not mandatory to successfully expand HSC, since cell populations with moderate levels of enrichment readily increased CD34 expression ex‐vivo, generating higher stem/progenitor cell yields. Overall, our findings stress the importance of establishing a balance between the cell proliferative potential and cell recovery upon purification, towards the efficient and cost‐effective expansion of HSC for cellular therapy. J. Cell. Biochem. 112: 1822–1831, 2011. © 2011 Wiley‐Liss, Inc.
CCN6 enhances ICAM‐1 expression and cell motility in human chondrosarcoma cells
Authors: Fong Yi‐Chin , Lin Chih‐Yang , Su Yi‐Chang , Chen Wen‐Chi , Tsai Fuu‐Jen , Tsai Chang‐Hai , Huang Chih‐Yang , Tang Chih‐Hsin 
Published: March 2011

AbstractChondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. CCN6 is a cysteine‐rich protein that belongs to the CCN (Cyr61, CTGF, and Nov) family of matricellular proteins. However, the effects of CCN6 on human chondrosarcoma cells are largely unknown. In this study, we found that CCN6 increased the migration and the expression of intercellular adhesion molecule‐1 (ICAM‐1) in human chondrosarcoma cells. αvβ3 and αvβ5 integrin monoclonal antibody and mitogen‐activated protein kinase (MEK) inhibitors (PD98059 and U0126) inhibited the CCN6‐induced increase of the migration and ICAM‐1 up‐regulation of chondrosarcoma cells. CCN6 stimulation increased the phosphorylation of focal adhesion kinase (FAK), MEK, and extracellular signal‐regulated kinase (ERK). In addition, activator protein‐1 (AP‐1) inhibitors suppressed the cell migration and ICAM‐1 expression enhanced by CCN6. Moreover, CCN6 increased AP‐1 luciferase activity and binding of c‐Jun to the AP‐1 element on the ICAM‐1 promoter. Taken together, our results indicate that CCN6 enhances the migration of chondrosarcoma cells by increasing ICAM‐1 expression through the αvβ3 and αvβ5 integrin receptor, FAK, MEK, ERK, c‐Jun, and AP‐1 signal transduction pathway. J. Cell. Physiol. 227: 223–232, 2012. © 2011 Wiley Periodicals, Inc.
High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid
Authors: Wang Dan , Li Qiang , Song Ziyu , Zhou Wei , Su Zhiguo , Xing Jianmin 
Published: March 2011

AbstractBACKGROUND: Succinic acid is a valuable four‐carbon organic chemical with applications in many fields. It was found that cell mass was an important factor in succinic acid production by metabolically engineered Escherichia coli strains. In this work, high cell density fermentation was investigated for succinic acid production by a metabolically engineered strain SD121 with ldhA, pflB, ptsG mutation and heterogenous cyanobacterial ppc overexpression.RESULTS: Under two‐stage cultivation, the controlled DO feeding strategy during the aerobic growth phase facilitated biomass up to a dry cell weight of 19.6 g L−1, and enhanced succinic acid production in the following anaerobic fermentation phase to a concentration of 116.2 g L−1. A near theoretical maximum succinic acid yield of 1.73 mol mol−1 glucose was achieved with an average productivity of 1.55 g L−1 h−1.CONCLUSION: The results indicated the potential advantage of high cell density fermentation for improvement of succinic acid production by E. coli. Copyright © 2010 Society of Chemical Industry
Hydrogel composites with temperature induced phase transition for biocatalysis
Authors: Wack Holger , Nellesen Anke , Schwarze‐Benning Kerstin , Deerberg Görge 
Published: March 2011

AbstractBACKGROUND: This paper focuses on the development of temperature induced phase transition hydrogels based on poly(N‐isopropylocrylamide (PNIPA) copolymers and their application as an immobilization matrix for biocatalysts.RESULTS: PNIPA‐co‐AAc and PNIPA‐co‐MAAc hydrogels were synthesized with different comonomer concentrations and analysed. In order to evaluate the capacity of hydrogels to take up or to release liquids the mass exchange capacity is introduced. In the presented work mass exchange capacities up to 22.4 were realized. To enhance the mechanical stability, composites of hydrogels and cellulosic fleeces were prepared. The enzyme Lipozyme® TL 100 L (Thermomyces languniosa Lipase) was successfully inserted into the hydrogels and into the hydrogel composites. Lipase‐catalysed transesterification of rapeseed oil with ethanol to the corresponding ethyl ester was investigated to prove the activity of immobilized enzyme and thus, the concept. The activity was found to be similar to that of free enzyme.CONCLUSION: Smart hydrogel composites were used for the transesterification of oil. Based on the results obtained, reversible loadable and mechanical stable hydrogel composites could be developed for continuous working reactor concepts. Copyright © 2010 Society of Chemical Industry
Solid–liquid extraction of terbium from phosphoric acid solutions using solvent‐impregnated resin containing TOPS 99
Authors: Kumar Batchu Nagaphani , Radhika Surampally , Kantam Mannepalli Lakshmi , Reddy Bontha Ramachandra 
Published: March 2011

AbstractBACKGROUND: In this work, the solid‐liquid extraction of terbium from phosphoric acid solutions using solvent‐impregnated resin containing TOPS 99, an equivalent of di‐2‐ethylhexyl phosphoric acid, has been investigated. The parameters studied include equilibration time, acid concentration, amount of resin, metal concentration, kinetics, temperature, loading, elution, regeneration and recycling.RESULTS: FT‐IR results confirm the physical interaction of the extractant with the resin. The extraction of terbium with TOPS 99 impregnated Amberlite XAD 4 resin was acid dependent and transfer of metal follows a cation exchange mechanism. The loading capacity of TOPS 99‐impregnated resin for terbium was calculated to be 23.8 mg g−1 resin. Controlling mechanism of the adsorption was found to be a chemical reaction following pseudo‐second‐order kinetics. The endothermic nature of extraction was confirmed by temperature studies. Among the various eluants studied, H2SO4 was the best. Regeneration and recycling of the resin indicated the resin can be used for continuous cycles.CONCLUSIONS: Terbium was successfully extracted from phosphoric acid using TOPS 99 extractant impregnated into Amberlite XAD4 with a maximum loading of 23.8 mg g−1 resin and fully recovered with 1 mol L−1sulfuric acid. The resin was subjected to seven cycles of extraction and elution without any loss of performance. Further studies showed that terbium could be separated from lutetium. Copyright © 2010 Society of Chemical Industry
Decomposition of hydrogen peroxide in the presence of activated carbons with different characteristics
Authors: Aguinaco Almudena , Pocostales Juan Pablo , García‐Araya Juan F. , Beltrán Fernando J. 
Published: March 2011

AbstractBACKGROUND: Catalytic ozonation promoted by activated carbon is a promising advanced oxidation process used in water treatment. Hydrogen peroxide generated as a by‐product from the reaction of ozone with some surface groups on the activated carbon or from the oxidation of some organic compounds present in the water being treated seems to play a key role in the catalytic ozonation process. Hydrogen peroxide decomposition promoted by two granular activated carbons (GAC) of different characteristics (Hydraffin P110 and Chemviron SSP‐4) has been studied in a batch reactor. The operating variables investigated were the stirring speed, temperature, pH and particle size. Also, the influence of metals on the GAC surface, that can catalyze hydrogen peroxide decomposition, was observed.RESULTS: Chemviron SSP‐4 showed a higher activity to decompose hydrogen peroxide than HydraffinP110 (70 and 50% of hydrogen peroxide removed after 2 h process, respectively). Regardless of the activated carbon used, hydrogen peroxide decomposition was clearly controlled by the mass transfer, although temperature and pH conditions exerted a remarkable influence on the process. Catalytic ozonation in the presence of activated carbon and hydrogen peroxide greatly improved the mineralization of oxalic acid (a very recalcitrant target compound). About 70% TOC (total organic carbon) depletion was observed after 1 h reaction in this combined system, much higher than the mineralization achieved by the single processes used.CONCLUSIONS: Of the two activated carbons studied, Chemviron SSP‐4 with an acidic nature presented a higher activity to decompose hydrogen peroxide. However the influence of the operating variables was quite similar in both cases. Experiments carried out in the presence of tert‐butanol confirmed the appearance of radical species. A kinetic study indicated that the process was controlled by the internal mass transfer and the chemical reaction on the surface of the activated carbon. The catalytic activity of hydrogen peroxide in oxalic acid ozonation promoted by activated carbon (O3/AC/H2O2) was also studied. The results revealed the synergetic activity of the system O3/AC/H2O2 to remove oxalic acid. Copyright © 2010 Society of Chemical Industry
Sox‐9 facilitates differentiation of adipose tissue‐derived stem cells into a chondrocyte‐like phenotype in vitro
Authors: Yang Zhe , Huang Chun‐Yuh. Charles , Candiotti Keith A. , Zeng Xiaoling , Yuan Taiyi , Li Jinliang , Yu Hong , Abdi Salahadin 
Published: March 2011

AbstractThe purpose of this study is to test whether ectopic expression of Sox‐9 can induce adipose tissue‐derived stem cells (ASCs) to function as real nucleus pulposus (NP) cells in vitro. Adenoviral vectors expressing Sox‐9 were reported to infect the chondroblastic and human disc cells, which resulted in increased Sox‐9 and type II collagen production. ASCs were isolated from rat inguinal adipose pad, characterized, and transduced in vitro with a retroviral vector encoding the Sox‐9 gene. Sox‐9‐engineered ASCs (ASCs/Sox‐9) were induced for the chondrocyte‐like cell differentiation by 3D cultured in alginate beads and TGF‐β3 for 2 weeks. Expression of exogenous Sox‐9 protein was detected. Type II collagen and Aggrecan gene expressions of induced ASCs/Sox‐9 were measured using real‐time PCR; proteoglycans expressions were measured by checking the glycosaminoglycan content and type II collagen production by enzyme‐linked immunosorbent assay. Isolated ASCs were CD 29+/CD44+/C‐Kit−/Lin−/CD34−/CD45−. ASCs/Sox‐9 expressed marked increase in exogenous Sox‐9 protein. After induction, type II collagen gene expression was sevenfold higher in mRNA levels, with an approximately twofold increase in protein levels of ASCs/Sox‐9 compared to ASCs. Type II collagen and proteoglycan productions were significantly increased in the ASCs/Sox‐9 compared to the ASCs. In addition, co‐culture of induced ASCs/Sox‐9 with matured NP cells resulted in enhanced increase in proteoglycan and type II collagen production. Constitutive retroviral expression of Sox‐9 could efficiently induce ASCs differentiation into chondrocyte‐like cells. This novel approach may provide a practicable system for a simple and rapid differentiation of ASCs into chondrocyte‐like cells which may be potentially used as a stem cell‐based therapeutic tool for the treatment of degenerative disc diseases. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1291–1297, 2011
Histopathological, clinical, and electrophysiological features influencing postoperative outcomes in carpal tunnel syndrome
Authors: Galasso Olimpio , Mariconda Massimo , Donato Giuseppe , Di Mizio Giulio , Padua Luca , Brando Antonio , Conforti Francesco , Valentino Paola , Gasparini Giorgio 
Published: March 2011

AbstractData on the outcome predictors of surgical result of carpal tunnel syndrome (CTS) is sparse and often conflicting. The purpose of this prospective comparative study was to evaluate the neurophysiologic findings and histological characteristics of the flexor tenosynovium in patients with CTS, assessing possible associations between these findings and preoperative patient status or surgical outcome. We evaluated 30 consecutive patients with a diagnosis of idiopathic CTS who were referred for surgery. Demographic data, subjective and objective data, and responses on an expanded assessment set, were collected before and 6 months after surgery. All patients underwent diagnostic neurophysiological testing prior to surgery, and histological analysis of tenosynovium specimens that were removed during surgery. Ten fresh‐frozen cadavers served as controls. There was significant improvement in most of the disease specific (Boston questionnaire and Hi‐Ob scale) and health generic (SF‐36 questionnaire) evaluation tools used in the study at the 6‐month follow‐up. Vascular changes were noted in the tenosynovium of the flexor tendon of patients with CTS when compared with controls. Greater synovial vascularization was negative predictor of the SF‐36's physical summary score before surgery. Bilateral presentation of symptoms and low nerve conduction velocity were negative predictors of the quality of life of patients after surgery for CTS. These findings have to be considered when discussing with patients as for the expected and desired outcomes of CTS surgery. The increased vascularization of flexor tenosynovium is associated with patients' functional status. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1298–1304, 2011
Network meta‐analysis of parametric survival curves
Authors: Ouwens Mario J. N. M. , Philips Zoe , Jansen Jeroen P. 
Published: March 2011

AbstractTo inform health‐care decision‐making, treatments are often compared by synthesizing results from a number of randomized controlled trials. The meta‐analysis may not only be focused on a particular pairwise comparison, but can also include multiple treatment comparisons by means of network meta‐analysis.For time‐to‐event outcomes such as survival, pooling is typically based on the hazard ratio (HR). The proportional hazards assumption that underlies current approaches of evidence synthesis is not only often implausible, but can also have a huge impact on decisions based on differences in expected outcomes, such as cost‐effectiveness analysis. The application of a constant HR implies the assumption that the treatment only has an effect on one characteristic of the survival distribution, while commonly used survival distributions, like the Weibull distribution, have both a shape and a scale parameter.Instead of using constant HRs, this paper proposes meta‐analysis of treatment effects based on the shape and scale parameters of parametric survival curves. The model for meta‐analysis is extended for network meta‐analysis and illustrated with an example. Copyright © 2011 John Wiley & Sons, Ltd.
Tumor vascularization and histopathologic regression of soft tissue sarcomas treated with isolated limb perfusion with TNF‐α and melphalan
Authors: Grabellus Florian , Kraft Corinna , Sheu‐Grabellus Sien‐Yi , Bauer Sebastian , Podleska Lars E. , Lauenstein Thomas C. , Pöttgen Christoph , Konik Margarethe J. , Schmid Kurt W. , Taeger Georg 
Published: March 2011

AbstractBackgroundIsolated limb perfusion (TM‐ILP) achieves high response rates in soft tissue sarcomas (STS). Some tumors show an insufficient association between radiological and pathological response. We investigated STS after TM‐ILP with a primary emphasis on histologic regression patterns.MethodsIn 53 patients with STS, TM‐ILP with subsequent tumor resection was performed. Regression was assessed by the Salzer‐Kuntschik regression scale. Microvessel density (MVD) of primary biopsies of 37 patients was determined by immunohistochemistry. Tumor regression was correlated with MVD of primary biopsies and other clinico‐pathological parameters.ResultsRegression presented mainly as necrosis or fibrosis/sclerosis upon histopathology. MFH, leiomyosarcoma, or clear cell sarcoma (CCS) responded well; whereas liposarcomas, synovial sarcomas, or MPNST were poor responders. MFH often had abundant necrosis; while other STS mainly presented with fibrosis/sclerosis. MVD had no influence on regression grade but modulated histologic regression patterns. Excellent regression demonstrated a trend toward an association with improved survival and local control.ConclusionTM‐ILP yielded high response rates in STS. Regression after TM‐ILP exhibits MVD‐dependent histopathologic patterns and variable efficacy in different sarcoma types. Complete regression seems to be a favorable prognostic factor. A concerted consideration of histopathology and clinical findings may contribute to a better clinical assessment of regression after TM‐ILP. J. Surg. Oncol. 2011; 103:371–379. © 2011 Wiley‐Liss, Inc.
Tracer agents for the detection of sentinel lymph nodes in breast cancer: Current concerns and directions for the future
Authors: Kelley Leah M. , Holmes Dennis R. 
Published: March 2011

AbstractTracer agents play a central role in axillary lymphatic mapping for sentinel node identification during breast cancer surgery. This article reviews the data concerning safety, efficacy, and availability of tracers currently in use. Alternate tracer materials are introduced, with review of the current literature. J. Surg. Oncol. 2011;104:91–96. © 2011 Wiley‐Liss, Inc.
Separation of regioisomers and enantiomers of underivatized saturated and unsaturated fatty acid monoacylglycerols using enantioselective HPLC
Authors: García Patricia , Franco Pilar , Álvarez Rosana , de Lera Ángel R. 
Published: March 2011

AbstractThe challenging separation of regioisomers and enantiomers of monoacylglycerols (MAGs) of fatty acids has been achieved using a single chiral HPLC column (Chiralpak® IC or Chiralpak® IA) and hexane/2‐propanol eluent mixtures, without previous derivatization of the samples. The efficacy of the method is independent of the chain length and the degree of unsaturation of the fatty acids of the MAG. In addition, the separation method allows monitoring the time course for the loss of enantiomeric purity of the fatty acid‐derived MAGs in polar hydroxylic solvents.
Cytokine Loaded Biopolymers as a Novel Strategy to Study Stem Cells during Wound‐Healing Processes
Authors: Grimaldi Annalisa , Banfi Serena , Vizioli Jacopo , Tettamanti Gianluca , Noonan Douglas M. , de Eguileor Magda 
Published: March 2011

AbstractThe biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin‐8 (IL‐8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morphofunctional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine‐loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step. magnified image
Enzyme‐Catalyzed Synthesis of a Novel Thermosensitive Polyester with Pendant Ketoprofen
Authors: Wang Hai‐Yang , Zhou Yu‐Jie , Wang Zao , Wang Na , Li Kun , Yu Xiao‐Qi 
Published: March 2011

AbstractA novel thermosensitive polyester with pendant ketoprofen was synthesized by copolymerization of PEG 800 with 2‐ketoprofendimethylmalate using CAL‐B at 70 °C. The products were characterized by NMR spectra and GPC. The in vitro study demonstrated that the polyester could release ketoprofen in physiological conditions, which indicates that the polyester could be a promising prodrug. magnified image
Ricci flow with hyperbolic warped product metrics
Authors: Ma Li , Xu Xingwang 
Published: March 2011

AbstractIn this short note, we show an example that the negative curvature is preserved in the deformation of hyperbolic warped product metrics under Ricci flow. It is showed that the flow converges to a flat metric in the sense of Cheeger‐Gromov as time going to infinity. © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
A Superhydrophobic to Superhydrophilic In Situ Wettability Switch of Microstructured Polypyrrole Surfaces
Authors: Chang Jean H. , Hunter Ian W. 
Published: March 2011

AbstractWe present an electrochemical layered system that allows for the fast, in situ wettability switch of microstructured PPy upon the application of an electric stimulus. We have eliminated the need for PPy to be immersed in an electrolyte to switch between wetting states, laying the groundwork for PPy to be used as a viable material in many applications, including microfluidics or smart textiles. The PPy surface was switched from the superhydrophobic state (contact angle = 159) to the superhydrophilic state (contact angle = 0) in 3 s. A wettability gradient was also created on a PPy surface using the layered system, causing a 3 µL droplet to travel approximately 2 mm in 0.8 s. magnified image
Opto‐Mechanical Effect in Photoactive Nematic Side‐Chain Liquid‐Crystalline Elastomers
Authors: Sánchez‐Ferrer Antoni , Merekalov Alexey , Finkelmann Heino 
Published: March 2011

AbstractThe mechanical behaviour of monodomain nematic side‐chain liquid‐crystalline elastomers containing azoderivatives as pendant groups or crosslinkers has been studied under UV irradiation and in the darkness at different temperatures. From the evaluation of the opto‐mechanical experiments, the mechanical efficiency, kinetic rates, activation energies and the isomerization mechanism of the azocompounds in the liquid‐crystalline matrix could be determined, as well as the effect of the chemical constitution of the azobenzene derivatives and their role in the elastomeric network. magnified image