bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of crosslink‐type tag‐probe pairs for fluorescent imaging of proteins
Authors: Nomura Wataru , Mino Tomoaki , Narumi Tetsuo , Ohashi Nami , Masuda Akemi , Hashimoto Chie , Tsutsumi Hiroshi , Tamamura Hirokazu 
Published: August 2010

AbstractUseful methods of protein labeling via functional peptide tags have been developed in the field of proteome and chemical biology. New tag‐probe pairs based on leucine zipper peptides for labeling target proteins are described. These consist of an α‐helical probe peptide with an environmental‐sensitive fluorescent dye and two antiparallel α‐helical tag peptides, and may be crosslinked, from the Cys residue of the tag peptide to the Nα‐chloroacetyl group of the probe peptide. Binding of the probe peptide to the tag peptides results in movement of the fluorophore from a hydrophilic to a hydrophobic environment inside the leucine zipper structure, causing a dramatic fluorescent change, mediated by the binding of the two peptides. As a spacer between the Nα‐chloroacetyl group and the original probe sequence, a single Gly residue was the most suitable among 0‐2 Gly residues. Crosslinking leads to superior fluorescence response, binding affinity, and chemical stability. These ZIP tag‐probe pairs are useful for labeling and fluorescent imaging of proteins. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 843–852, 2010.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Towards biomolecular assembly employing extended native chemical ligation in combination with thioester synthesis using an N→S acyl shift
Authors: Ackrill Tom , Anderson David W. , Macmillan Derek 
Published: August 2010

AbstractThe advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N‐terminal cysteine residue and a C‐terminal thioester. The reactivity of the thioester component can dictate how fragments behave through careful choice of leaving group (alkyl or aryl thiol) and the C‐terminal amino acid residue. Although thioester reactivity is exquisitely controlled, reactivity of the N‐terminal cysteine residue has been curbed using protecting groups, usually the thiazolidine‐4‐carboxo (Thz) group as it can be removed in the presence of the thioester at acidic pH. Only recently has the concept of orthogonal ligation been extended to thiol auxiliary mediated Native Chemical Ligation (a.k.a. Extended Ligation) which, owing to their inherent difference in reactivity, have allowed peptides to be selectively extended at the C‐terminus without recourse to protecting groups on the N‐terminus. Herein we explored the compatibility of acyl transfer auxiliaries with peptide thioester production via an N→S acyl shift for this purpose. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 495–503, 2010.
Measurement of kon without a rapid‐mixing device
Authors: Kahn James , Dutnall Robert N. , Matulef Kimberly , Plesniak Leigh A. 
Published: August 2010

AbstractWe have recently designed a biochemistry laboratory experiment for the purpose of providing students an advanced experience with enzyme kinetics and the kinetics of binding. Bestatin, a well‐known and commercially available general protease inhibitor, is a slow‐binding inhibitor of aminopeptidase isolated from Aeromonas proteolytica. The binding is on a timescale slow enough for measurement without the use of a rapid‐mixing device. Aminopeptidase inhibition is detected via a standard colorimetric assay with an inexpensive commercially available substrate. The binding of bestatin follows first order binding kinetics with a rate constant kon of 59 ± 5 M−1 s−1. This aminopeptidase is well characterized with several crystal structures and a published Ki, which students can then use to calculate the value for koff.
A Theoretical Study of the Mechanism of the Desymmetrization of Cyclic meso‐Anhydrides by Chiral Amino Alcohols
Authors: Dedeoglu Burcu , Catak Saron , Houk K. N. , Aviyente Viktorya 
Published: August 2010

AbstractThe alcoholysis of cyclic meso‐anhydrides catalyzed by β‐amino alcohols has been investigated with DFT quantum mechanics to determine the mechanism of this reaction. Both nucleophilic catalysis and general base catalysis pathways are explored for methanol‐induced ring opening of an anhydride catalyzed by a chiral amino alcohol. The nucleophilic pathway involves a late transition state with a high energy barrier. In this mechanism, methanolysis is expected to take place following the amine‐induced ring opening of the anhydride. In the base‐catalyzed mechanism, methanol attack on one carbonyl group of the meso‐anhydride is assisted by the β‐amino alcohol; the amine functionality abstracts the methanol proton. The chiral amino alcohol also catalyzes the reaction by stabilizing the oxyanion that forms upon ring opening of the anhydride by hydrogen bonding with its alcoholic moiety. Both stepwise and concerted pathways have been studied for the general base catalysis route. Transition structures for both are found to be lower in energy than in the nucleophilic mechanism. Overall this study has shed light on the mechanism of the β‐amino alcohol‐catalyzed alcoholysis of cyclic meso‐anhydrides, showing that the nucleophilic pathway is approximately 100 kJ mol−1 higher in energy than the general base pathway.
Handling of Polymer Particles in Microchannels
Authors: Marcati A. , Serra C. , Bouquey M. , Prat L. 
Published: August 2010

AbstractThis paper deals with solid‐liquid operations in microchannels. Continuous operations on solids (modification of frequency, change of solvent, encapsulation) in order to handle polymer particles in microchannels are described in terms of the limits of operating conditions and their possible applications. A methodology to design and implement operations on polymer particles is presented here. It is applied for the generation of onion‐like structures. A microdevice completely built with Plexiglas plates and fused silica capillaries is used. The device includes droplet generation, polymerization and microparticle flow manipulation. The particles manipulated are in the range of 70 to 200 microns with a very low index of polydispersity.
Simulation of a Fluidized‐Bed Reactor for Dimethyl Ether Synthesis
Authors: Kumar M. , Srivastava V. C. 
Published: August 2010

AbstractDimethyl ether (DME) has received increasing interest as a potential substitute for diesel and liquefied petroleum gas. The production of DME from syngas is exothermic in nature overall, and has a narrow operational window in fixed‐bed reactors. Consequently, fluidized‐bed reactors, which have high heat and mass transfer efficiencies, are major current areas of investigation for DME production from syngas. In the present paper, the comprehensive reactor model proposed by Mahecha‐Botero et al. [1] has been modified and employed to simulate DME synthesis in a fluidized‐bed reactor. The modified model has been employed under various operating conditions to maximize CO conversion (XCO), DME productivity and DME selectivity with respect to methanol.
ADLOC: An Aptamer‐Displacement Assay Based on Luminescent Oxygen Channeling
Authors: Niebel Björn , Lentz Christian , Pofahl Monika , Mayer Günter , Hoerauf Achim , Pfarr Kenneth M. , Famulok Michael 
Published: August 2010

AbstractFunctional nucleic acids, such as aptamers and allosteric ribozymes, can sense their ligands specifically, thereby undergoing structural alterations that can be converted into a detectable signal. The direct coupling of molecular recognition to signal generation enables the production of versatile reporters that can be applied as molecular probes for various purposes, including high‐throughput screening. Here we describe an unprecedented type of a nucleic acid‐based sensor system and show that it is amenable to high‐throughput screening (HTS) applications. The approach detects the displacement of an aptamer from its bound protein partner by means of luminescent oxygen channeling. In a proof‐of‐principle study we demonstrate that the format is feasible for efficient identification of small drug‐like molecules that bind to a protein target, in this case to the Sec7 domain of cytohesin. We extended the approach to a new cytohesin‐specific single chain DNA aptamer, C10.41, which exhibits a similar binding behavior to cytohesins but has the advantage of being more stable and easier to synthesize and to modify than the RNA‐aptamer M69. The results obtained with both aptamers indicate the general suitability of the aptamer‐displacement assay based on luminescent oxygen channelling (ADLOC) for HTS. We also analyzed the potential for false positive hits and identified from a library of 18 000 drug‐like small molecules two compounds as strong singlet‐oxygen quenchers. With full automation and the use of commercially available plate readers, we estimate that the ADLOC‐based assay described here could be used to screen at least 100 000 compounds per day.
ChemInform Abstract: Magnesium in Methanol Selective Reduction of a Conjugate Double Bond in an α,β‐Unsaturated Ester Related to Pregnadiene.
Authors: ZARECKI A. , WICHA J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Rhodium Complexes as Catalysts in Diels‐Alder Reactions.
Authors: CARMONA D. , CATIVIELA C. , GARCIA‐CORREAS R. , LAHOZ F. J. , LAMATA M. P. , LOPEZ J. A. , LOPEZ‐RAM DE VIU M. P. , ORO L. A. , SAN JOSE E. , VIGURI F. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel Chiral Super‐Lewis Acidic Catalyst for Enantioselective Synthesis.
Authors: HAYASHI Y. , ROHDE J. J. , COREY E. J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: CrCl2 Mediated Addition of Allylic Halides or Phosphates to N‐ Protected α‐Amino Aldehydes. Stereocontrolled Synthesis of a New Core for C2 Symmetric HIV‐Protease Inhibitors.
Authors: CIAPETTI P. , FALORNI M. , TADDEI M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Titanium‐Catalyzed Cascade Carboalumination of 2‐Alkyl‐Substituted Dienes and Related Trienes.
Authors: KONDAKOV D. Y. , WANG S. , NEGISHI E. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: (2 + 2) Photocycloaddition of Two Captodative Alkenes to 3‐ Acetylthianaphthene.
Authors: DOEPP D. , HASSAN A. A. , HENKEL G. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cobalt‐Catalyzed Radical Oxygenation with Molecular Oxygen.
Authors: BAMHAOUD T. , PRANDI J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hydroalkylation of Imines by Cyclohepta‐1,3,5‐triene.
Authors: YUNNIKOVA L. P. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Diastereoseelective Synthesis of Heterosubstituted Organogembismetallic Reagents. Application to a New Propargylmetalation Reaction of Vinyl Metals.
Authors: BRASSEUR D. , MAREK I. , NORMANT J.‐F. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Facile Synthesis of Dinitro(2.2)metacyclophane (IIa) and Its Partial Reduction.
Authors: TSUGE A. , MORIGUCHI T. , MATAKA S. , TASHIRO M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Achieving Excellent Tradeoffs Among Optical, Chemical and Thermal Properties in Second‐Order Nonlinear Optical Chromophores.
Authors: RAO V. P. , JEN A. K.‐Y. , CAI Y. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 1,4‐Oxathiocines and Thiopyrans by the Reaction of 2‐ Amino‐4,5‐dihydro‐3‐thiophenecarbonitriles with Ethyl Diazoacetoacetate.
Authors: YAMAGATA K. , AKIZUKI K. , YAMAZAKI M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structure‐Odor Correlation. Part 24. Synthesis and Olfactory Properties of Damascone and Damascenone Analogues.
Authors: WEYERSTAHL P. , LICHA K. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Tetranorfriedolabdane Diterpene from Vellozia stipitata.
Authors: PINTO A. C. , ANTUNES O. A. C. , PIZZOLATTI M. G. , RUMJANEK V. M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Total Syntheses of (.+‐.)‐β‐Pinguisene (XIII) and (.+‐.)‐ Pinguisenol (XV).
Authors: SCHINZER D. , RINGE K. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 1,5‐Anhydro‐2,3‐dideoxy‐2‐(guanin‐9‐yl)‐D‐arabino‐hexitol.
Authors: DECLERCQ R. , HERDEWIJN P. , VAN MEERVELT L. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nucleosides and Nucleotides. Part 148. New Neplanocin Analogues. Part 6. Synthesis and Potent Antiviral Activity of 6′‐Homoneplanocin A.
Authors: SHUTO S. , OBARA T. , SAITO Y. , ANDREI G. , SNOECK R. , DE CLERCQ E. , MATSUDA A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystallographically Defined Pore Systems. Reaction Vessels with Molecular Dimensions
Authors: SCHUETH F. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Transition‐Metal‐Directed Assembly of Well‐Defined Organic Architectures Possessing Large Voids: From Macrocycles to (2)Catenanes
Authors: FUJITA M. , OGURA K. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Towards the Synthesis of Monocyclic Carbon Clusters: (2 + 2) Cycloreversion of Propellane‐Annulated Dehydroannulenes
Authors: TOBE Y. , FUJII T. , MATSUMOTO H. , NAEMURA K. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Baylis‐Hillman Reaction: A Novel Carbon‐Carbon Bond Forming Reaction
Authors: BASAVAIAH D. , RAO P. D. , HYMA R. S. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Do You Really Know What Your Drug Looks Like or How It Reacts?
Authors: LOCK C. J. L. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reductive Amination of Aldehydes and Ketones with Sodium Triacetoxyborohydride. Studies on Direct and Indirect Reductive Amination Procedures.
Authors: ABDEL‐MAGID A. F. , CARSON K. G. , HARRIS B. D. , MARYANOFF C. A. , SHAH R. D. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel Method of C‐C Bond Formation via Phenylation of Terminal Acetylenes by Triphenylbismuth Difluoride.
Authors: LERMONTOV S. A. , RAKOV I. M. , ZEFIROV N. S. , STANG P. J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Analysis of Polar Organic Compounds Using Charge Exchange Ionization and Membrane Introduction Mass Spectrometry.
Authors: CISPER M. E. , GARRETT A. W. , CAMERON D. , HEMBERGER P. H. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Improved Procedure for the Enantioselective Synthesis of (+)‐ and (‐ )‐cis‐4‐Hydroxyprolines and Bulgecinines.
Authors: GRAZIANI L. , PORZI G. , SANDRI S. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Shikimate Pathway. Part 8. Synthesis of (‐)‐3(R)‐Amino‐4(R),5(R)‐ dihydroxy‐1‐cyclohexene‐1‐carboxylic Acid: The 3(R)‐Amino Analogue of ( ‐)‐Shikimic Acid.
Authors: ADAMS H. , BAILEY N. A. , BRETTLE R. , CROSS R. , FREDERICKSON M. , HASLAM E. , MACBEATH F. S. , DAVIES G. M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Comparison of the Sensitivities of Various Derivatives of Oligosaccharides in LC/MS with Fast Atom Bombardment and Electrospray Ionization Interfaces.
Authors: SUZUKI S. , KAKEHI K. , HONDA S. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.