bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative analysis of organosulphur compounds in crude oil samples using magnetic solid phase extraction based on Au-Fe3O4 adsorbent and gas chromatography with time-of-flight mass spectrometry
Authors: Mgiba Samuel S. , Mhuka V. , Hintsho-Mbita N. C. , Mketo Nomvano 
Published: May 2024

AbstractThis study focused on the development of a magnetic solid phase extraction (m-SPE) method using Au-Fe3O4 as an adsorbent followed by GC-ToFMS analysis for the determination of organosulphur compounds (OSCs) in fuel samples. The m-SPE using Au-Fe3O4 NPs was preferred because of the low toxicity of the adsorbent1, high separation efficiency using external magnet2 and greater extraction selectivity between sulphur and Au atom3. The Au-Fe3O4 NPs were characterized using XRD, UV–Vis, TEM, SEM and FTIR. This method was optimized using multivariate analysis based on a two-level full factorial and central composite designs. The conditions which produced optimum efficiency were found to be 150 mg mass of sorbent, 100 µL eluent volume, 50 min extraction time and 6,5 pH of the sample. These optimum conditions showed a relatively low limit of detection in the range of 0.02–0.199nµg/g and limit of quantification of 0.08–0.602 µg/g. Furthermore, a relative standard deviation of triplicates analysis was between 0.8 and 2.3% with good linearity of 0.9816–0.9961. The percentage recovery for thiophene, 3-methylthiophene, benzothiophene and dibenzothiophene ranged from 76 to 95% for the spiked samples. The optimized m-SPE method was then applied in real fuel oil samples. The concentration of thiophene, 3-methylthiophene, benzothiophene and dibenzothiophene in crude oil, gasoline, diesel and kerosene ranged from 0.43–1.94 µg/g, 0.78–1.63 µg/g, 0.95–4.31 µg/g to 1.55–2.09 µg/g, respectively. The m-SPE, followed by GC-ToFMS method, proved to be efficient, inexpensive and an alternative method for OSCs analysis in fuel oils.
On the Relaxation of Gauss’s Capillarity Theory Under Spanning Conditions
Authors: Novack Michael 
Published: May 2024

AbstractWe study a variational model for soap films in which the films are represented by sets with fixed small volume rather than surfaces. In this problem, a minimizing sequence of completely “wet" films, or sets of finite perimeter spanning a wire frame, may converge to a film containing both wet regions of positive volume and collapsed (dry) surfaces. When collapsing occurs, these limiting objects lie outside the original minimization class and instead are admissible for a relaxed problem. Here we show that the relaxation and the original formulation are equivalent by approximating the collapsed films in the relaxed class by wet films in the original class.
New method for assigning cardinal weights in multi-criteria decision-making: the constant weight ratio method
Authors: Casanovas-Rubio Maria del Mar , Vinolas Bernat 
Published: May 2024

AbstractA new method is proposed to convert ordinal ranking of a number of criteria and an additional piece of information into numerical weights. A literature review of methods for assigning cardinal weights based on ordinal ranking is performed, as well as an analysis of their behaviour. The new method, called ‘constant weight ratio’ (CWR), enables better adjustment to the decision-maker’s preferences than purely ordinal ranking methods. It also solves the problem of the excessive decrease in the weight of the most important criterion (or criteria) when the total number of criteria is large and the weight of the most important criterion (or criteria) must be high. It is achieved via three simple steps and flexible input data. The additional piece of information may be: (i) the relative importance of the criteria, i.e., the weight ratio, (ii) the total weight of the most important set of criteria, or (iii) the weight of the most important criterion. The proposed method is applied to two case studies in the cultural sector to illustrate that the resulting weights are equivalent to other methods requiring more input data from the decision maker.
Neurophenomenology in Action: Integrating the First-Person Perspective into the Libet Experiment
Authors: Schmidt Stefan , Bauer Prisca R. , Trautwein Fynn-Mathis 
Published: May 2024

AbstractIn this conceptual contribution, we argue that experimental investigations of phenomena in the cognitive sciences and consciousness research may benefit from a thorough integration of data acquired from the first-person perspective. We present a line of research from our lab applying this approach to the Libet task on voluntary action. In this well-known experimental paradigm, participants perform a movement at a moment of their own choice. Previous research has shown that this voluntary decision to perform the action is preceded by a specific pattern in the electroencephalogram, the so-called readiness potential. This finding that the decision is preceded (and presumably determined) by an action-related brain processes puts the neuroscientific account at odds with our subjective intuition and challenges the notion of free will. This discrepancy exemplifies the gap between neuro-cognitive models of the mind and the accounts of our conscious experience. The aim of our theoretical proposal is to enrich the study of volitional action by integrating reports from the first-person perspective with the Libet paradigm to develop a more coherent account. This provides an example of implementing the research program of neurophenomenology developed by Francisco Varela to overcome the gap between scientific accounts of the mind and subjective experience. Specifically, we show how this can be achieved by interweaving three methodological approaches: (i) adapting common neuro-cognitive paradigms (i.e., the Libet task); (ii) employing refined first-person methods such as the micro-phenomenological interview; and (iii) collaborating with experienced meditators as research participants. Our contribution demonstrates how the neurophenomenological framework can be used to shed new light on long-standing and fundamental debates in consciousness research. We show that this approach not only addresses questions of intellectual curiosity but also has concrete ethical implications for the practice of science itself, self-determination, and the accountability of the conscious subject. On the basis of our approach, meditation can be seen as a method for enhancing self-regulation and self-determination, which allows for more deliberate decisions and thus more ethical behavior.
ZNF692 promotes the migration and response to immunotherapy of clear cell renal cell carcinoma cells by targeting metabolic pathway
Authors: Liu Yuming , Zeng Dehua , Gao Yunzhen 
Published: May 2024

AbstractClear cell renal cell carcinoma (ccRCC), with high mortality and poor prognosis, is the most common type of renal malignancy. It is necessary to identify new biomarkers that can serve as indicators for the detection of ccRCC at its early stages. In this study, we analyzed the role of classical zinc finger protein 692 (ZNF692) in ccRCC using datasets from The Cancer Genome Atlas (TCGA) and Single Cell Portal and immunohistochemical (IHC) staining of a tissue-microarray, and analyzed the function of ZNF692 in ccRCC cells. The analyses indicated that ZNF692 was upregulated in ccRCC samples compared with normal or paracancerous control samples (P < 0.001) and that the expression of this gene was linked to poor overall survival (HR = 2.1, P < 0.0001). The knockdown of ZNF692 inhibited the proliferation and migration of ccRCC cells by target GTPase-activating protein (SH3 domain)-binding protein 2 (G3BP2), and transmembrane 9 superfamily member 2 (TM9SF2)). T, B, proximal, and collecting tubule cells are the dominant cell types in normal kidney tissue where ZNF692 is expressed. In addition, immune checkpoint blockade (ICB) therapy dramatically changed the expression patterns of ZNF692. Collectively, these data indicate that ZNF692 may serve as prognosis, and as a potential indicator of the response to ICB therapy, a possibility needs to be verified by a case‒control study.
Prognostic significance and immune landscape of a cell cycle progression-related risk model in bladder cancer
Authors: Tuo Zhouting , Lin Yuan , Zhang Ying , Gao Liang , Yu Dexin , Wang Jiani , Sun Chenyu , Sun Xianchao , Wang Jinyou , Prasad Apurwa , Bheesham Nimarta , Meng Muzi , Lv Zhengmei , Chen Xin 
Published: May 2024

Abstract
Background
A greater emphasis has been placed on the part of cell cycle progression (CCP) in cancer in recent years. Nevertheless, the precise connection between CCP-related genes and bladder cancer (BCa) has remained elusive. This study endeavors to establish and validate a reliable risk model incorporating CCP-related factors, aiming to predict both the prognosis and immune landscape of BCa.

Methods
Clinical information and RNA sequencing data were collected from the GEO and TCGA databases. Univariate and multivariate Cox regression analyses were conducted to construct a risk model associated with CCP. The performance of the model was assessed using ROC and Kaplan–Meier survival analyses. Functional enrichment analysis was employed to investigate potential cellular functions and signaling pathways. The immune landscape was characterized using CIBERSORT algorithms. Integration of the risk model with various clinical variables led to the development of a nomogram.

Results
To build the risk model, three CCP-related genes (RAD54B, KPNA2, and TPM1) were carefully chosen. ROC and Kaplan–Meier survival analysis confirm that our model has good performance. About immunological infiltration, the high-risk group showed decreased levels of regulatory T cells and dendritic cells coupled with increased levels of activated CD4 + memory T cells, M2 macrophages, and neutrophils. Furthermore, the nomogram showed impressive predictive power for OS at 1, 3, and 5 years.

Conclusion
This study provides new insights into the association between the CCP-related risk model and the prognosis of BCa, as well as its impact on the immune landscape.
Molecular mechanism of MLCK1 inducing 5-Fu resistance in colorectal cancer cells through activation of TNFR2/NF-κB pathway
Authors: Tang Huifen , Zhou Hui , Zhang Liang , Tang Tingting , Li Ning 
Published: May 2024

Abstract
Background and aims
Chemotherapy resistance in colorectal cancer have been faced with significant challenges in recent years. Particular interest is directed to tumor microenvironment function. Recent work has, identified a small molecule named Divertin that prevents myosin light chain kinase 1(MLCK1) recruitment to the perijunctional actomyosin ring(PAMR), restores barrier function after tumor necrosis factor(TNF)-induced barrier loss and prevents disease progression in experimental inflammatory bowel disease. Studies have shown that MLCK is a potential target for affecting intestinal barrier function, as well as for tumor therapy. However, the relative contributions of MLCK expression and chemotherapy resistance in colorectal cancers have not been defined.

Methods
Statistical analysis of MYLK gene expression differences in colorectal cancer patients and normal population and prognosis results from The Cancer Genome Atlas(TCGA) data. Cell activity was detected by Cell counting Kit-8. Cell proliferation was detected by monoclonal plate. The apoptosis was detected by flow cytometry and western blot. Determine the role of MLCK1 in inducing 5-Fluorouracil(5-Fu) resistance in colorectal cancer cells was detected by overexpression of MLCK1 and knock-down expression of MLCK1.

Results
MLCK1 is expressed at different levels in different colorectal cancer cells, high MLCK1 expressing cell lines are less sensitive to 5-Fu, and low MLCK1 expressing cell lines are more sensitive to 5-Fu. MLCK1 high expression enhances resistance to 5-Fu in colorectal cancer cells and the sensitivity to 5-Fu was increased after knocking down the expression of MLCK1, that might be closely correlated to TNFR2/NF-κB pathway.

Conclusions
MLCK1 high expression can enhance resistance to 5-Fu in colorectal cancer cells and the sensitivity to 5-Fu was increased after knocking down the expression of MLCK1, that might be closely correlated to TNFR2/NF-κB pathway, which will provide a new method for the treatment of colorectal cancer patients who are resistant to 5-Fu chemotherapy.
Bridging and bonding social capital in place-based rural careers advising
Authors: Fuqua Melyssa 
Published: May 2024

Abstract
Research studies continue to document the inequitable access to quality careers education and guidance for rural Australian youth, raising questions about these programs in rural schools. A significant proportion of the existing rural careers education and guidance literature focuses on factors of students’ decision-making, with little attention on the role of the careers advisor. This article explores how rural careers advisors develop and leverage their social capital to create place-based careers education and guidance programs. Drawing on narrative interviews with career advisors in western Victoria, the article uses theories of bridging and bonding social capital as an analytic framework to highlight the risks and opportunities in creating place-based programs. It argues that local knowledge and a strong network of relationships across the local communities assists advisors in tailoring relevant and effective careers education and guidance. This is vital to ensuring rural students have access to the quality careers education and guidance they need to have positive post-school outcomes.
Experimental study on the effect of filament-extrusion rate on the structural, mechanical and thermal properties of material extrusion 3D-printed polylactic acid (PLA) products
Authors: Lendvai László , Fekete Imre , Rigotti Daniele , Pegoretti Alessandro 
Published: May 2024

AbstractMaterial extrusion (MEX), also commonly referred to as fused deposition modeling (FDM) or fused filament fabrication (FFF) is currently one of the most commonly used additive manufacturing techniques. The quality of the 3D-printed objects fabricated by MEX methods highly relies on various printing parameters, one of which is the so-called filament extrusion multiplier (k). In this study, 3D-printed parts were prepared by MEX technique during which the material feeding rate was adjusted by varying the extrusion multiplier in the range of 97–105% (k = 0.97–1.05). The fabricated parts were tested for their geometrical, structural, mechanical, and thermal conductivity properties. Based on computed tomographic analysis and scanning electron microscopic images, increasing the k parameter resulted in smaller voids, along with gradually decreasing porosity (from 5.82 to 0.05%). Parallel to the decreasing defects, the thermal conductivity of the parts improved from 0.157 to 0.188 W/mK as determined by light-flash analysis technique. On the other hand, when k was set to ≥ 1.03 the geometrical accuracy declined, the size of the specimens considerably increased relative to the nominal values, especially in the X–Y directions due to excess material getting “squeezed” on the sides of the specimens. This latter phenomenon also resulted in the formation of a number of stress concentration sites, which manifested in the decrease of mechanical properties. Accordingly, the tensile, flexural, and impact strength of the samples improved up to k = 1.03; however, above that it dropped considerably.
Towards sustainable precipitation management in Madurai Town Planning Area (India)
Authors: Thiruchelve S. R. , Chandran S. , Irannezhad M. , Anandhi A. , Veluswamy Kumar , Surendran U. 
Published: May 2024

AbstractThis study aimed to look for efficient development strategies towards achieving sustainable precipitation management in the Madurai Town Planning Area (MTPA) in India. In the MTPA, precipitation is the predominant source of freshwater for agricultural and domestic uses in addition to water received through conventional systems. The average annual precipitation in the region is ~ 816 mm with standard deviation of 233 mm. The regression analysis showed that annual precipitation is following a decreasing trend and will be about 716 mm by 2030. The Precipitation Concentration Index (PCI) values ranging from 18 to 20 represented strong irregularities and seasonality in annual precipitation over the study area. March, July, September, and November months contributed 2.5, 6.19, 14, and 16% to annual precipitation, respectively, and all showed decreasing trends over time. Only 20% of Northeast monsoon was active over the past 44 years (1976–2019), resulting in a 15% probability of flood and drought occurrences throughout the MTPA. The long-term (1976–2019) average value of annual precipitation days was about 42. The Concentration Index (CI) showed that 60–70% of precipitation was received within 25% of precipitable days. Daily precipitation class of moderate (7.5–34 mm) and rather heavy (34–64 mm) ranges were the dominant types of precipitation, contributing 46% and 26% to total annual precipitation, respectively. Except for October, the monthly precipitation received was deficit to meet the irrigation water demand in the MTPA. However, development of precipitation harvesting systems can annually preserve about 27 Mm3 freshwater in the urban space of the MTPA.
Exploring the complexities of plant UV responses; distinct effects of UV-A and UV-B wavelengths on Arabidopsis rosette morphology
Authors: Cunningham Natalie , Crestani Gaia , Csepregi Kristóf , Coughlan Neil E. , Jansen Marcel A. K. 
Published: May 2024

AbstractUV-B radiation can substantially impact plant growth. To study UV-B effects, broadband UV-B tubes are commonly used. Apart from UV-B, such tubes also emit UV-A wavelengths. This study aimed to distinguish effects of different UV-B intensities on Arabidopsis thaliana wildtype and UVR8 mutant rosette morphology, from those by accompanying UV-A. UV-A promotes leaf-blade expansion along the proximal–distal, but not the medio-lateral, axis. Consequent increases in blade length: width ratio are associated with increased light capture. However, petiole length is not affected by UV-A exposure. This scenario is distinct from the shade avoidance driven by low red to far-red ratios, whereby leaf blade elongation is impeded but petiole elongation is promoted. Thus, the UV-A mediated elongation response is phenotypically distinct from classical shade avoidance. UV-B exerts inhibitory effects on petiole length, blade length and leaf area, and these effects are mediated by UVR8. Thus, UV-B antagonises aspects of both UV-A mediated elongation and classical shade avoidance. Indeed, this study shows that accompanying UV-A wavelengths can mask effects of UV-B. This may lead to potential underestimates of the magnitude of the UV-B induced morphological response using broadband UV-B tubes.
Graphical abstract
Explaining Alport syndrome—lessons from the adult nephrology clinic
Authors: Mabillard Holly , Ryan Rebecca , Tzoumas Nik , Gear Susie , Sayer John A. 
Published: May 2024

AbstractAlport syndrome is a genetic kidney disease that causes worsening of kidney function over time, often progressing to kidney failure. Some types of Alport syndrome cause other symptoms and signs, including hearing loss and eye abnormalities. Research now indicates that Alport syndrome (autosomal dominant inheritance) is the most common form. Alport syndrome can have X-linked or a rare form of autosomal recessive inheritance. Traditionally, a kidney biopsy was used to diagnose Alport syndrome, but genetic testing provides a more precise and less invasive means of diagnosis and reveals the underlying pattern of inheritance. At present, there are no specific curative treatments for Alport syndrome however there is a strong international effort in pursuit of future therapies. Currently, angiotensin-converting enzyme inhibitors (ACEi), or an angiotensin receptor blocker (ARB) if a patient cannot tolerate an ACEi, slow down the progression of kidney disease and can delay the onset of kidney failure by years. There are other potential treatments in research that potentially can help delay the onset of kidney issues. Early treatment of patients and identification of their at-risk relatives is a priority. People living with Alport syndrome and their doctors now benefit from an active international research community working on translating further treatments into clinical practice and providing up-to-date clinical guidelines.
Two new Neotetraonchus species (Dactylogyridea, Dactylogyridae) parasitising the Peruvian sea catfish Galeichthys peruvianus (Siluriformes, Ariidae), including molecular data
Authors: Cruces C. , Chero J. , Ñacari L.A. , De Celis V.R. , Simões R. , Luque J.L. 
Published: May 2024

Abstract
As part of a parasitological survey, several specimens of two new monopisthocotylean species, Neotetraonchus celsomanueli sp. nov. and N.peruvianus sp. nov. (Dactylogyridea, Dactylogyridae), were collected from the gill filaments of the Peruvian sea catfish Galeichthys peruvianus (Siluriformes, Ariidae) off Puerto Pizarro, Tumbes region, Peru. Neotetraonchus celsomanueli sp. nov. is characterised by an MCO with a T-shaped distal end and an accessory piece that is ribbed and expanded proximally with a worm-shaped termination. Neotetraonchus peruvianus sp. nov. is typified by its MCO, which has a sledgehammer-shaped distal end and an accessory piece with a claw-shaped distal end. Additionally, N.peruvianus sp. nov. is characterised by its jellyfish-shaped onchium. A partial 28S rDNA sequence was obtained from N.celsomanueli sp. nov., and a phylogenetic analysis was conducted. This analysis revealed the phylogenetic position of Neotetraonchus celsomanueli sp. nov. within a clade comprising monopisthocotylean parasites of diadromous and marine ariid catfishes, including Hamatopeduncularia spp., Chauhanellus spp., Thysanotohaptor Kritsky, Shameem, Kumari & Krishnaveni, 2012, and Neocalceostomoides spinivaginalis Lim, 1995. This finding brings the number of known Neotetraonchus species to seven and represents the first described Neotetraonchus species infecting marine catfishes from Peru.
Morphological and molecular data on Pseudozoogonoides ugui Shimazu, 1974 (Digenea: Microphalloidea: Zoogonidae) ex Pseudaspius hakonensis (Günther, 1877) and taxonomic problems in Zoogoninae genera
Authors: Atopkin D.M. , Ivashko Y.I. , Izrailskaia A.V. , Tatonova Y.V. , Besprozvannykh V.V. 
Published: May 2024

Abstract
New morphological and molecular data were generated for trematodes recovered from the intestines of the fish Pseudaspius hakonensis from two locations in the south of the Russian Far East. Morphologically, these trematodes are identical to Pseudozoogonoides ugui (Microphalloidea: Zoogonidae) from Japan. According to results of phylogenetic analysis based on 28S rDNA sequence data, P. ugui was closely related to Zoogonoides viviparus, and P. subaequiporus appears as a sister taxon to these two species. Genetic distance values, calculated based on both 28S rDNA and ITS2 rDNA, between P. ugui and Z. viviparus represents an interspecific differentiation level. Our results have an ambiguous explanation, indicating that the implication of the presence of one or two compact vitellarial aggregations for the differentiation of Zoogonoides and Pseudozoogonoides should be reconsidered or that our results open up the question of the taxonomical status of trematodes previously denoted as Z. viviparus and P. subaequiporus.