bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stage-specific antigens ofTrichinella spiralis
Authors: Parkhouse R. M. E. , Ortega-Pierres Guadalupe 
Published: August 2011

SUMMARYInfective larvae, adults and newborn larvae ofTrichinella spiraliswere surface labelled with radioactive iodine, and the surface material was solubilized in the mild detergent sodium deoxycholate. The radio-isotope labelled products were stage-specific glycoproteins that were few in number (2–4 components) and antigenic in infected mice and rats. Antibodies synthesized in infected animals against these biochemically defined surface antigens may or may not interact with the surface of the living worm. The latter type of antibody is unlikely to be involved in the initial phase of parasite rejection and is therefore another example of a non-protective host antibody response. The stimulus for its synthesis must be the observed release of surface antigen. A monoclonal antibody to a surface glycoprotein of newborn larvae protected against infection, and also promoted eosinophil killingin vitro. This observation emphasizes the importance of surface antigens in protection against infection, suggests a role for granulocytesin vivo, and provides encouragement for the possible use of nematode surface antigens in protection. An example of regional specialization of the nematode cuticle was given by a monoclonal antibody reactive with only the surface of the male intromittent organ and not the female or remainder of the male. The same stages were labelledin vitrowith radioactive methionine, and the secreted proteins were also found to be stage-specific. Some, but not all, were antigenic in infected mice. The total concanavalin A-binding somatic glycoproteins of each stage exhibited considerable individuality, and hence stage specificity, when resolved by two-dimensional gel electrophoresis. These results extend our knowledge of stage-specific components ofT. spiralis, and allow a rational approach towards protection and the construction of diagnostic procedures.
Variation in ‘target’ antigens between appropriate and inappropriate hosts of Taenia saginata metacestodes
Authors: Harrison L. J. S. , Parkhouse R. M. E. , Sewell M. M. H. 
Published: August 2011

SUMMARYThe antibody response of the appropriate hosts (cattle) to Taenia saginata larvae was compared with that of an inappropriate host (Balb/c mice) using gel electrophoresis followed by immunoelectrotransfer blot techniques (Western blotting). Three groups of cattle were included, those known to be resistant to challenge infection because of repeated oral challenge with T. saginata eggs over a 1-year period, a previously infected but known susceptible group and a group of uninfected controls. Serum from the mice and the two groups of infected cattle contained antibodies recognizing different ‘target’ antigens, some of which may be related to host resistance. The potential value of the technique of Western blotting in dissecting the humoral response of a particular host species to parasitic infection and in the identification of those antigens suitable for the production of effective vaccines is discussed.
Antigens ofPlasmodiwn falciparumblood stages with clinical interest cloned and expressed inE. coli
Authors: Scaife J. , Bone N. , Goman M. , Hall R. , Hope I. A. , Hyde J. E. , Langsley G. , Mackay M. , Oquendo P. , Simmons D. 
Published: August 2011

The human malaria parasite,Plasmodium falciparum, is currently being actively studied by molecular biologists. It is hoped that the use of recombinant DNA techniques in this area will give new insights into the biology of the organism and, at the same time, provide new approaches to diagnosis and vaccine development.Our own studies employ the blood stages of the parasite and cover three main areas: enzymes of importance in parasite metabolism; antigens of potential use in a subunit vaccine; and repetitive DNA as a probe able to distinguish genetically different isolates ofP. falciparumand as a species-specific diagnostic tool in human and mosquito infections.
Global warming: Trends and effects
Authors: Tickell Crispin 
Published: August 2011

SUMMARYAs animals we have been a remarkably successful species; but also as animals we are vulnerable to environmental, in particular climate change. Such change is accelerating as a result of human activity, and global warming may already be taking place. Although we can foresee the trends, we cannot yet be specific about the results. Change usually proceeds by steps rather than gradients. But warming would probably include new risks to human health and contribute to an increase in human displacement. Of course climate change is only one among other complex problems facing human society, but it is closely related to them all, including population increase, environmental degradation and loss of biodiversity. We cannot prevent global warming but we can anticipate and mitigate some of its worst effects. Peoples and governments still need persuading of the need for action and of the magnitude of the issue at stake.
Transmission and control in an institutional pandemic influenza A(H1N1) 2009 outbreak
Authors: ARINAMINPATHY N. , RAPHAELY N. , SALDANA L. , HODGEKISS C. , DANDRIDGE J. , KNOX K. , McCARTHY N. D. 
Published: August 2011

SUMMARYA pandemic influenza A(H1N1) 2009 outbreak in a summer school affected 117/276 (42%) students. Residential social contact was associated with risk of infection, and there was no evidence for transmission associated with the classroom setting. Although the summer school had new admissions each week, which provided susceptible students the outbreak was controlled using routine infection control measures (isolation of cases, basic hygiene measures and avoidance of particularly high-risk social events) and prompt treatment of cases. This was in the absence of chemoprophylaxis or vaccination and without altering the basic educational activities of the school. Modelling of the outbreak allowed estimation of the impact of interventions on transmission. These models and follow-up surveillance supported the effectiveness of routine infection control measures to stop the spread of influenza even in this high-risk setting for transmission.
The prevalence, detection and intervention for depression and anxiety in oncology
Authors: Boothroyd David Andrew , Hodgson Denyse 
Published: August 2011

AbstractClinical depression is the most prevalent psychiatric disorder amongst cancer patients and is associated with significant functional impairment, although often under-diagnosed and untreated. In one study, only 6% of patients with clinical depression were identified by their oncologists. The detection of and intervention for anxiety and depression in oncology is widely debated in the literature. Diagnosing clinically significant distress amongst cancer patients requires sensitivity as many symptoms of depression are very similar to those of some cancers themselves. The two detection methods discussed in the literature are either self report questionnaires (i.e., HADS) and diagnostic clinical interviews. There are several techniques described in the literature that have shown to be effective in reducing anxiety and depression in oncology. These can be broken down into four main categories, namely, cognitive behavioural therapy (CBT), counselling, drug therapy and complementary therapies, and it is acknowledged that patients receiving any type of intervention generally cope better than those who receive none at all. The effective management of anxiety and depression is dependent on the ability of health professionals to establish a rapport with patients and pick up on cues, regardless of whether intervention is necessary. However, an obvious lack of time and resources within the NHS can be a limiting factor, thus all health care professionals must take more responsibility for the detection of anxiety and depression, followed by the appropriate referral.
Prediction of specific virus outbreaks made from the increased concentration of a new class of virus genomic peptides, replikins.
Authors: Bogoch Samuel , Bogoch Elenore 
Published: August 2011

AbstractAdvance warning of pathogen outbreaks has not been possible heretofore. A new class of genomic peptides associated with rapid replication was discovered and named replikins. Software was designed to analyze replikins quantitatively. Replikin concentration changes were measured annually prior to, and “real time” every few days during, the 2009 H1N1 influenza pandemic. Replikins were seen by both linear sequence representation and three-dimensional X-ray diffraction, and found to expand on the virus hemagglutinin surface prior to and during the H1N1 pandemic.A highly significant increased concentration of virus replikins was found a) retrospectively in three pandemics from 1918 to 1999 (14,227 sequences)(p<0.001), and b) prospectively before the H1N1 2009 pandemic (12,806 sequences) (in the hemagglutinin gene (N=8,046), p values by t-test = 1/10130, by linear regression = 1/1024 and 1/1029, by Spearman correlation < 2/1016, by Wilcoxon rank sum<1/1016, by multiple regression adjusting for correlation between consecutive years = 2/1022. Rising replikin concentration in H1N1 from 2006 to 2008, predicted one year in advance the H1N1 outbreak of 2009; and in H5N1, predicted the lethal outbreaks of H5N1 1997-2010.The possible combination of influenza strains H1N1 (high infectivity) and H5N1 (high lethality) is a matter of global concern (1,2). The risk of a combined H1N1 (high infectivity) - H5N1 (high lethality) outbreak may have increased because first, the Replikin Counts of the two virus strains have risen simultaneously, not seen previously; second, the rise is to the highest levels recorded since 1918 for H1N1, in Mexico (16.7), and since 1957 for H5N1, in Egypt (23.3); and third, clinical outbreaks of each strain are occurring in 2011. These simultaneous conditions may increase the risk that the two virus strains might come into contact with each other more frequently, facilitating transfer of genomic material to form a hybrid.
Endothelin-Induced Sarcoplasmic Reticulum Calcium Depletion Waves in Vascular Smooth Muscle Cells
Authors: Esfandiarei Mitra , Choi Yohan , Tehrani Arash , Hoskins Jeremy , Fameli Nicola , van Breemen Cornelis 
Published: August 2011

AbstractAgonist-stimulated waves of elevated cytoplasmic Ca2+ concentration ([Ca2+]i ) regulate blood vessel tone and vasomotion in vascular smooth muscle. Previous studies employing cytoplasmic Ca2+ indicators revealed that these Ca2+ waves were generated by a combination of inositol 1,4,5-trisphosphate (IP3) and Ca2+ induced Ca2+ release (CICR) from the sarcoplasmic reticulum (SR); although, some of the mechanistic details remain uncertain. However, these findings were derived indirectly from observing agonist-induced [Ca2+]i fluctuations in the cytoplasm.Here, for the first time, we have recorded Endothelin-1 (ET-1) induced waves of Ca2+ depletion from the SR lumen in vascular smooth muscle cells (VSMCs) using a calsequestrin-targeted Ca2+ indicator. Our findings show that these waves: (1) are due to regenerative CICR by the receptors for IP3 (IP3R), (2) have a marked latency period, (3) are characterized by a transient increase in SR Ca2+ ([Ca2+]SR ) both at the point of origin and at the wave front, (4) proceed with diminishing velocity, and (5) are arrested by the nuclear envelope. Our quantitative model indicates that the gradual decrease in the velocity of the SR depletion wave, in the absence of external Ca2+, results from continuity of the SR luminal network.
Racemization as a stereochemical measure of dynamics and robustness in shape-shifting organic molecules
Authors: He Maggie , Bode Jeffrey W. 
Published: August 2011

Bullvalene is a structurally unique dynamic molecule thought to interconvert among 1.2 million degenerate isomers. The incorporation of different chemical substituents onto the bullvalene core should lead to a “shape-shifting” molecule that can interconvert among thousands of discrete structural isomers. Previous NMR spectroscopy and HPLC studies on substituted bullvalenes ascertained the fact that these compounds are dynamic, but they could not attest to whether the molecules are only interconverting among only a few isomers or if a multitude of structures are being accessed. Here we confirm the remarkable shape-shifting property of a tetrasubstituted bullvalene by means of a racemization experiment. We show that a single, though fleeting, chiral, enantioenriched tetrasubstituted bullvalene isomer can spontaneously equilibrate to a racemic population of dynamic compounds. Despite the fact that conversion from one enantiomer of a bullvalene isomer to the other may require dozens or even hundreds of rearrangements and involve many potential pathways, CD spectroscopy and HPLC analysis of different bullvalene populations showed that multiple pathways exist and result in the complete racemization of an initial enantioenriched chiral bullvalene. These oligosubstituted bullvalenes represent a very rare example of an entity that can spontaneously transform itself into different discrete structures using ambient thermal energy. The confirmation that these shape-shifting organic molecules are chemically robust yet structurally dynamic is an important step toward their further use as materials, sensors, and biologically active compounds.
Shelf life and time horizons in the historiography of linguistics
Authors: Cram David 
Published: August 2011

SummaryThe aim of this paper is to outline an approach to linguistic historiography which is informed by 20th-century phenomenology and hermeneutics, but which is formulated in linguistic terms. The key concept is that of the ‘time horizons’ which delimit the context which is relevant to the interpretation of an event or text. The idea of the time horizon is here illustrated by the everyday notion of the shelf-life of consumable items. The main thrust of the argument is that the interpretation of historical events and texts necessarily involves not one but two cognitive contexts, that of the historical protagonist and that of the modern observer. The approach sets up a three-level contrast between chronography (getting the facts right), reconstruction (recounting the historical story), and interpretation (making sense of the past story in terms of present-day understandings).
Letters, sounds and things
Authors: Isermann Michael M. 
Published: August 2011

SummaryFor a long time, and well beyond the publication of John Wilkins’sEssay Towards a Real Character(1668), a latent tension characterised the orthographic tradition. It was caused by two incompatible principles whose problematic relationship had never been addressed. One is the idea of the logical, chronological and semiotic primacy of speech over writing, or of sound over character, a principle which lives on in modern linguistics. The other is the doctrine of an abstract unity of sound/potestasand character/figurain the letter/littera, an axiom that appears to have been abandoned only with the emancipation of modern phonetics from its former orthographic frame of reference. With the advent of the real-character movement in the 17th century, a way was suddenly opened up for the problematic issue to be discussed and resolved. Since a real, i.e., non-sound-related, character implied at least a non-priority of speech over writing — if not a priority of writing —, the first principle could be reformulated so as to be made consonant with the idea of an abstractlittera. This paper tries to bring out Wilkins’s sophisticated discussion of thelittera, his attempt to localise the abstract unity of sound and character in the configuration of the articulatory organs, as well as the utilisation of hislitteraconcept for his design of a real character of sounds. In a parallel line of argument, it is claimed that Wilkins’s orthography, or doctrine of letters, forms the conceptual equivalent to his metaphysics, or science of things. More than that, the two disciplines are so interlocked that things and letters can be seen to converge in letter-things, or thing-letters.
Pronouncing the “P”
Authors: Brewer Charlotte 
Published: August 2011

SummaryDuring the course of the 19th century, many English dictionaries carrying information on pronunciation directed that Greek-derived words beginning ps- should be pronounced with a silent initialp. In the relevant section of theOED, however (published 1909), the editor J. A. H. Murray (1837–1915) advised that, contrary to general practice, pronouncing the p was preferable, since it made clear the etymology of such words thus enhancing their intelligibility. Dictionaries after theOEDhave reportedpas an optional pronunciation for many years subsequently — even as late as the 1970s — though their conflicting evidence supports the hypothesis that pronouncing the p was a dictionary chimaera never adopted by more than a handful of pedantic philologists. The article concludes that claims to descriptivism rather than prescriptivism, even by the most reputable dictionaries, should be taken with a pinch of salt.
The work of Richard John Lloyd (1846–1906) and “the crude system of doctrine which passes at present under the name of Phonetics”
Authors: MacMahon Michael K. C. 
Published: August 2011

SummaryRichard John Lloyd (1846–1906) was a well-known Liverpool businessman who also pursued the study of phonetics, English language, literature, sociology and philosophy. His wide intellectual background embraced mathematics and physics too. His work in phonetics is characterised by a particular emphasis on the acoustics of vowel sounds, as well as by close attention to the fine detail of articulation (what he called “minute phonetics”). Alongside a small group of scientists in Europe, he was actively involved in research into the formant structure of vowels. His relatively early death meant that his work never achieved the prominence it deserved, and hence his influence has remained negligible. His work, however, bears comparison with that of colleagues in articulatory and experimental phonetics later in the 20th century.
Algonquian and Indo-European gender in a Historiographic Perspective
Authors: Kilarski Marcin 
Published: August 2011

SummaryThis article examines shared motifs in the history of the study of grammatical gender in North American Indian and Indo-European languages. Specifically, I investigate the degree of semantic and cultural motivation attributed to gender in Algonquian languages, and present analogies with accounts of gender in Indo-European. The presence of exceptions within animate gender in Algonquian has led to conflicting interpretations: while some focused on the arbitrary nature of the categorization, others regarded them as culturally based. Algonquian languages provide an example of how claims that have traditionally been made about Indo-European gender, particularly its supposed semantic arbitrariness, have been extended to languages apparently less suited for the purpose.
Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering
Authors: Benedetti Manuel , Leggio Claudia , Federici Luca , De Lorenzo Giulia , Pavel Nicolae Viorel , Cervone Felice 
Published: August 2011

Abstract
We report here the low-resolution structure of the complex formed by the endo-polygalacturonase from Fusarium phyllophilum and one of the polygalacturonase-inhibiting protein from Phaseolus vulgaris after chemical cross-linking as determined by small-angle x-ray scattering analysis. The inhibitor engages its concave surface of the leucine-rich repeat domain with the enzyme. Both sides of the enzyme active site cleft interact with the inhibitor, accounting for the competitive mechanism of inhibition observed. The structure is in agreement with previous site-directed mutagenesis data and has been further validated with structure-guided mutations and subsequent assay of the inhibitory activity. The structure of the complex may help the design of inhibitors with improved or new recognition capabilities to be used for crop protection.
Voriconazole versus itraconazole for antifungal prophylaxis following allogeneic haematopoietic stem‐cell transplantation
Authors: Marks David I. , Pagliuca Antonio , Kibbler Christopher C. , Glasmacher Axel , Heussel Claus‐Peter , Kantecki Michal , Miller Paul J.S. , Ribaud Patricia , Schlamm Haran T. , Solano Carlos , Cook Gordon ,   for the IMPROVIT Study Group
Published: August 2011

SummaryAntifungal prophylaxis for allogeneic haematopoietic stem‐cell transplant (alloHCT) recipients should prevent invasive mould and yeast infections (IFIs) and be well tolerated. This prospective, randomized, open‐label, multicentre study compared the efficacy and safety of voriconazole (234 patients) versus itraconazole (255 patients) in alloHCT recipients. The primary composite endpoint, success of prophylaxis, incorporated ability to tolerate study drug for ≥100 d (with ≤14 d interruption) with survival to day 180 without proven/probable IFI. Success of prophylaxis was significantly higher with voriconazole than itraconazole (48·7% vs. 33·2%, P < 0·01); more voriconazole patients tolerated prophylaxis for 100 d (53·6% vs. 39·0%, P < 0·01; median total duration 96 vs. 68 d). The most common (>10%) treatment‐related adverse events were vomiting (16·6%), nausea (15·8%) and diarrhoea (10·4%) for itraconazole, and hepatotoxicity/liver function abnormality (12·9%) for voriconazole. More itraconazole patients received other systemic antifungals (41·9% vs. 29·9%, P < 0·01). There was no difference in incidence of proven/probable IFI (1·3% vs. 2·1%) or survival to day 180 (81·9% vs. 80·9%) for voriconazole and itraconazole respectively. Voriconazole was superior to itraconazole as antifungal prophylaxis after alloHCT, based on differences in the primary composite endpoint. Voriconazole could be given for significantly longer durations, with less need for other systemic antifungals.
Strict regulation of morphological differentiation and secondary metabolism by a positive feedback loop between two global regulators AdpA and BldA in Streptomyces griseus
Authors: Higo Akiyoshi , Horinouchi Sueharu , Ohnishi Yasuo 
Published: August 2011

SummaryAdpA is a global transcriptional regulator that is induced by the microbial hormone A‐factor and activates many genes required for morphological differentiation and secondary metabolism in Streptomyces griseus. We confirmed that the regulatory tRNA gene bldA was required for translation of TTA‐containing adpA. We also demonstrated that AdpA bound two sites upstream of the bldA promoter and activated transcription of bldA. Thus, we revealed a unique positive feedback loop between AdpA and BldA in S. griseus. Forced expression of bldA in an A‐factor‐deficient mutant resulted in the partial restoration of aerial mycelium formation and streptomycin production, suggesting that the positive feedback loop could prevent premature transcriptional activation of the AdpA‐target genes in the wild‐type strain. We revealed that the morphological defect of the bldA mutant could be attributed mainly to the TTA codons of only two genes: adpA and amfR. amfR encodes a transcriptional activator essential for aerial mycelium formation and is a member of the AdpA regulon. Thus, amfR is regulated by a feedforward mechanism involving AdpA and BldA. We concluded that the central regulatory unit composed of AdpA and BldA plays important roles in the initiation of morphological differentiation and secondary metabolism triggered by A‐factor.