bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of apoptosis in mineralizing murine versus avian micromass culture systems
Authors: Roy Rani , Kudryashov Valery , Binderman Itzhak , Boskey Adele L. 
Published: June 2010

AbstractChondrocyte apoptosis is thought to be an important step in the calcification of cartilage in vivo; however, there are conflicting reports as to whether or not this apoptosis is a necessary precursor to mineralization. The goal of this study was to determine whether or not apoptosis is necessary for mineralization in an in vitro murine micromass model of endochondral ossification. C3H10T1/2 murine mesenchymal stem cells were plated in micromass culture in the presence of 4 mM inorganic phosphate with the addition of the apoptogens, camptothecin, or staurosporine, to induce apoptosis. The rate and total accumulation of mineralization was measured with 45Ca uptake. In these studies, both apoptogens increased the rate of mineralization, with staurosporine increasing 45Ca accumulation by about 2.5 times that of controls and camptothecin increasing total amounts of mineralization about 1.5 times that of controls. Inhibiting cell apoptosis with the caspase inhibitor, ZVAD‐fmk, to prevent apoptosis, caused slower rates of 45Ca uptake; however, total amounts of 45Ca accumulation reached the same values by day 30 of culture. FTIR data showed mineralization in all samples treated with 4 mM inorganic phosphate, with the highest mineral to matrix ratios in the camptothecin treated samples. J. Cell. Biochem. 111: 653–658, 2010. © 2010 Wiley‐Liss, Inc.
Early response to ErbB2 over‐expression in polarized Caco‐2 cells involves partial segregation from ErbB3 by relocalization to the apical surface and initiation of survival signaling
Authors: Pfister Amber B. , Wood Robert C. , Salas Pedro J.I. , Zea Delma L. , Ramsauer Victoria P. 
Published: June 2010

AbstractIn several human cancers, ErbB2 over‐expression facilitates the formation of constitutively active homodimers resistant to internalization which results in progressive signal amplification from the receptor, conducive to cell survival, proliferation, or metastasis. Here we report on studies of the influence of ErbB2 over‐expression on localization and signaling in polarized Caco‐2 and MDCK cells, two established models to study molecular trafficking. In these cells, ErbB2 is not over‐expressed and shares basolateral localization with ErbB3. Over‐expression of ErbB2 by transient transfection resulted in partial separation of the receptors by relocalization of ErbB2, but not ErbB3, to the apical surface, as shown by biotinylation of the apical or basolateral surfaces. These results were confirmed by immunofluorescence and confocal microscopy. Polarity controls indicated that the relocalization of ErbB2 is not the result of depolarization of the cells. Biotinylation and confocal microscopy also showed that apical, but not basolateral ErbB2 is activated at tyrosine 1139. This phosphotyrosine binds adaptor protein Grb2, as confirmed by immunoprecipitation. However, we found that it does not initiate the canonical Grb2‐Ras‐Raf‐Erk pathway. Instead, our data supports the activation of a survival pathway via Bcl‐2. The effects of ErbB2 over‐expression were abrogated by the humanized anti‐ErbB2 monoclonal antibody Herceptin added only from the apical side. The ability of apical ErbB2 to initiate an altered downstream cascade suggests that subcellular localization of the receptor plays an important role in regulating ErbB2 signaling in polarized epithelia. J. Cell. Biochem. 111: 643–652, 2010. © 2010 Wiley‐Liss, Inc.
Effect of leukotriene D4 on mouse embryonic stem cell migration and proliferation: Involvement of PI3K/Akt as well as GSK‐3β/β‐catenin signaling pathways
Authors: Kim Min Hee , Lee Yu Jin , Kim Mi Ok , Kim Jin Sang , Han Ho Jae 
Published: June 2010

AbstractThe actual leukotriene D4 (LTD4) signaling pathways that regulate cell proliferation have not been elucidated thoroughly although fatty acid and its metabolites play a key role in regulations of embryonic functions. Thus, this study investigated the response of mouse embryonic stem (ES) cells exposed to LTD4 and elucidated the signaling pathways as well. LTD4 increased DNA synthesis in concentration‐dependent (≥10−7 M) and time‐dependent (≥12 h) manners, as determined by [3H] thymidine incorporation and increased cell number. LTD4 induced the phosphorylation of signal transducer and activator of transcription‐3 (STAT3) and the increase of intracellular Ca2+ levels via cysteinyl leukotriene (CysLT) 1 and 2 receptors. LTD4 increased Akt activation and calcineurin expression, which were blocked by STAT3 inhibitor and calcium chelators. LTD4‐induced glycogen synthase kinase (GSK)‐3β phosphorylation was decreased by LY294002, Akt inhibitor, and cyclosporine A. LTD4 inhibited the phosphorylation of β‐catenin. In addition, LTD4‐stimulated migration through increased activation of focal adhesion kinase (FAK) and paxillin which were blocked by Akt inhibitor and cyclosporine A. LTD4‐induced increases in protooncogene and cell cycle regulatory proteins were blocked by cyclosporine A, FAK siRNA, and β‐catenin siRNA. In conclusion, LTD4‐stimulated mouse ES cell proliferation and migration via STAT3, phosphoinositide 3‐kinases (PI3K)/Akt, Ca2+‐calcineurin, and GSK‐3β/β‐catenin pathway. J. Cell. Biochem. 111: 686–698, 2010. © 2010 Wiley‐Liss, Inc.
Protegrin‐1 orientation and physicochemical properties in membrane bilayers studied by potential of mean force calculations
Authors: Rui Huan , Im Wonpil 
Published: June 2010

AbstractProtegrin‐1 (PG‐1) belongs to the family of antimicrobial peptides. It interacts specifically with the membrane of a pathogen and kills the pathogen by releasing its cellular contents. To fully understand the energetics governing the orientation of PG‐1 in different membrane environments and its effects on the physicochemical properties of the peptide and membrane bilayers, we have performed the potential of mean force (PMF) calculations as a function of its tilt angle at four distinct rotation angles in explicit membranes composed of either DLPC (1,2‐dilauroylphosphatidylcholine) or POPC (1‐palmitoyl‐2‐oleoylphosphatidylcholine) lipid molecules. The resulting PMFs in explicit lipid bilayers were then used to search for the optimal hydrophobic thickness of the EEF1/IMM1 implicit membrane model in which a two‐dimensional PMF in the tilt and rotation space was calculated. The PMFs in explicit membrane systems clearly reveal that the energetically favorable tilt angle is affected by both the membrane hydrophobic thickness and the PG‐1 rotation angle. Local thinning of the membrane around PG‐1 is observed upon PG‐1 tilting. The thinning is caused by both hydrophobic mismatch and arginine‐lipid head group interactions. The two‐dimensional PMF in the implicit membrane is in good accordance with those from the explicit membrane simulations. The ensemble‐averaged Val16 15N and 13CO chemical shifts weighted by the two‐dimensional PMF agree fairly well with the experimental values, suggesting the importance of peptide dynamics in calculating such ensemble properties for direct comparison with experimental observables. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Leptin potentiates antiproliferative action of cAMP elevation via protein kinase A down‐regulation in breast cancer cells
Authors: Naviglio Silvio , Di Gesto Davide , Illiano Fausto , Chiosi Emilio , Giordano Antonio , Illiano Gennaro , Spina Annamaria 
Published: June 2010

AbstractPreviously, we have shown that leptin potentiates the antiproliferative action of cAMP elevating agents in breast cancer cells and that the protein kinase A (PKA) inhibitor KT‐5720 prevented the antiproliferative effects induced by the leptin plus cAMP elevation. The present experiments were designed to gain a better understanding about the PKA role in the antitumor interaction between leptin and cAMP elevating agents and on the underlying signaling pathways. Here we show that exposure of MDA‐MB‐231 breast cancer cells to leptin resulted in a strong phosphorylation of both ERK1/2 and STAT3. Interestingly, intracellular cAMP elevation upon forskolin pretreatment completely abrogated both ERK1/2 and STAT3 phosphorylation in response to leptin and was accompanied by a consistent CREB phosphorylation. Notably, leptin plus forskolin cotreatments resulted in a strong decrease of both PKA regulatory RIα and catalytic subunits protein levels. Importantly, pretreatment with the PKA inhibitor KT‐5720 blocked the forskolin‐induced CREB phosphorylation and prevented both the inhibition by forskolin of leptin‐induced ERK1/2 and STAT3 phosphorylation and the PKA subunits down‐regulation induced by the combination of leptin and forskolin. Altogether, our results indicate that leptin‐dependent signaling pathways are influenced by cAMP elevation and identify PKA as relevantly involved in the pharmacological antitumor interaction between leptin and cAMP elevating drugs in MDA‐MB‐231 cells. We propose a molecular model by which PKA confers its effects. Potential therapeutic applications by our data will be discussed. J. Cell. Physiol. 225: 801–809, 2010. © 2010 Wiley‐Liss, Inc.
Regulation of lipopolysaccharide‐induced inflammatory response and endotoxemia by β‐arrestins
Authors: Porter Katie J. , Gonipeta Babu , Parvataneni Sitaram , Appledorn Daniel M. , Patial Sonika , Sharma Deepika , Gangur Venugopal , Amalfitano Andrea , Parameswaran Narayanan 
Published: June 2010

Abstractβ‐Arrestins are scaffolding proteins implicated as negative regulators of TLR4 signaling in macrophages and fibroblasts. Unexpectedly, we found that β‐arrestin‐1 (β‐arr‐1) and ‐2 knockout (KO) mice are protected from TLR4‐mediated endotoxic shock and lethality. To identify the potential mechanisms involved, we examined the plasma levels of inflammatory cytokines/chemokines in the wild‐type (WT) and β‐arr‐1 and ‐2 KO mice after lipopolysaccharide (LPS, a TLR4 ligand) injection. Consistent with lethality, LPS‐induced inflammatory cytokine levels in the plasma were markedly decreased in both β‐arr‐1 and ‐2 KO, compared to WT mice. To further explore the cellular mechanisms, we obtained splenocytes (separated into CD11b+ and CD11b− populations) from WT, β‐arr‐1, and ‐2 KO mice and examined the effect of LPS on cytokine production. Similar to the in vivo observations, LPS‐induced inflammatory cytokines were significantly blocked in both splenocyte populations from the β‐arr‐2 KO compared to the WT mice. This effect in the β‐arr‐1 KO mice, however, was restricted to the CD11b− splenocytes. Our studies further indicate that regulation of cytokine production by β‐arrestins is likely independent of MAPK and IκBα‐NFκB pathways. Our results, however, suggest that LPS‐induced chromatin modification is dependent on β‐arrestin levels and may be the underlying mechanistic basis for regulation of cytokine levels by β‐arrestins in vivo. Taken together, these results indicate that β‐arr‐1 and ‐2 mediate LPS‐induced cytokine secretion in a cell‐type specific manner and that both β‐arrestins have overlapping but non‐redundant roles in regulating inflammatory cytokine production and endotoxic shock in mice. J. Cell. Physiol. 225: 406–416, 2010. © 2010 Wiley‐Liss, Inc.
A review of evidence on mobile use by micro and small enterprises in developing countries
Authors: Donner Jonathan , Escobari Marcela X. 
Published: June 2010

AbstractThe paper offers a systematic review of 14 studies of the use of mobile telephony by micro and small enterprises (MSEs) in the developing world, detailing findings about changes to enterprises' internal processes and external relationships, and findings about mobile use vs. traditional landline use. Results suggest that there is currently more evidence for the benefits of mobile use accruing mostly (but not exclusively) to existing MSEs rather than new MSEs, in ways that amplify existing material and informational flows rather than transform them. The review presents a more complete picture of mobile use by MSEs than was previously available, and identifies priorities for future research, including comparisons of the impact of mobile use across subsectors of MSEs and extensions beyond studies of existing enterprises. Copyright © 2010 John Wiley & Sons, Ltd.
The interaction of Jagged‐1 cytoplasmic tail with afadin PDZ domain is local, folding‐independent, and tuned by phosphorylation
Authors: Popovic Matija , Bella Juraj , Zlatev Ventsislav , Hodnik Vesna , Anderluh Gregor , Barlow Paul N. , Pintar Alessandro , Pongor Sándor 
Published: June 2010

AbstractJagged‐1, one of the five Notch ligands in man, is a membrane‐spanning protein made of a large extracellular region and a 125‐residue cytoplasmic tail bearing a C‐terminal PDZ recognition motif (1213RMEYIV1218). Binding of Jagged‐1 intracellular region to the PDZ domain of afadin, a protein located at cell–cell adherens junctions, couples Notch signaling with the adhesion system and the cytoskeleton. Using NMR chemical shift perturbation and surface plasmon resonance, we studied the interaction between the PDZ domain of afadin (AF6_PDZ) and a series of polypeptides comprising the PDZ‐binding motif. Chemical shift mapping of AF6_PDZ upon binding of ligands of different length (6, 24, and 133 residues) showed that the interaction is strictly local and involves only the binding groove in the PDZ. The recombinant protein corresponding to the entire intracellular region of Jagged‐1, J1_ic, is mainly disordered in solution, and chemical shift mapping of J1_ic in the presence of AF6_PDZ showed that binding is not coupled to folding. Binding studies on a series of 24‐residue peptides phosphorylated at different positions showed that phosphorylation of the tyrosine at position ‐2 of the PDZ‐binding motif decreases its affinity for AF6_PDZ, and may play a role in the modulation of this interaction. Finally, we show that the R1213Q mutation located in the PDZ‐binding motif and associated with extrahepatic biliary atresia increases the affinity for AF6_PDZ, suggesting that this syndrome may arise from an imbalance in the coupling of Notch signaling to the cytoskeleton. Copyright © 2010 John Wiley & Sons, Ltd.
Antecedents of work–family conflict: A meta‐analytic review
Authors: Michel Jesse S. , Kotrba Lindsey M. , Mitchelson Jacqueline K. , Clark Malissa A. , Baltes Boris B. 
Published: June 2010

AbstractThis study provides and meta‐analytically examines an organizing framework and theoretical model of work–family conflict. Results, based on 1080 correlations from 178 samples, indicate that work role stressors (job stressors, role conflict, role ambiguity, role overload, time demands), work role involvement (job involvement, work interest/centrality), work social support (organizational support, supervisor support, coworker support), work characteristics (task variety, job autonomy, family friendly organization), and personality (internal locus of control, negative affect/neuroticism) are antecedents of work‐to‐family conflict (WFC); while family role stressors (family stressors, role conflict, role ambiguity, role overload, time demands, parental demands, number of children/dependents), family social support (family support, spousal support), family characteristics (family climate), and personality (internal locus of control, negative affect/neuroticism) are antecedents of family‐to‐work conflict (FWC). In addition to hypothesized results, a revised model based on study findings indicates that work role stressors (job stressors, role conflict, role ambiguity, role overload) and work social support (organizational support, supervisor support, coworker support) are predictors of FWC; while family role stressors (family stressors, role conflict, role ambiguity, role overload), family involvement (family interest/centrality), family social support (family support, spousal support), and family characteristics (family climate) are predictors of WFC. Copyright © 2010 John Wiley & Sons, Ltd.
Biochemical T2* MR quantification of ankle arthrosis in pes cavovarus
Authors: Krause Fabian G. , Klammer Georg , Benneker Lorin M. , Werlen Stefan , Mamisch Tallal C. , Weber Martin 
Published: June 2010

AbstractPes cavovarus affects the ankle biomechanics and may lead to ankle arthrosis. Quantitative T2 STAR (T2*) magnetic resonance (MR) mapping allows high resolution of thin cartilage layers and quantitative grading of cartilage degeneration. Detection of ankle arthrosis using T2* mapping in cavovarus feet was evaluated. Eleven cavovarus patients with symptomatic ankle arthrosis (13 feet, mean age 55.6 years, group 1), 10 cavovarus patients with no or asymptomatic, mild ankle arthrosis (12 feet, mean age 41.8 years, group 2), and 11 controls without foot deformity (18 feet, mean age 29.8 years, group 3) had quantitative T2* MR mapping. Additional assessment included plain radiographs and the American Orthopaedic Foot and Ankle Society (AOFAS) score (groups 1 and 2 only). Mean global T2* relaxation time was significantly different between groups 1 and 2 (p = 0.001) and groups 1 and 3 (p = 0.017), but there was no significance for decreased global T2* values in group 2 compared to group 3 (p = 0.345). Compared to the medial compartment T2* values of the lateral compartment were significantly (p = 0.025) higher within group 1. T2* values in the medial ankle joint compartment of group 2 were significantly lower than those of group 1 (p = 0.019). Ankle arthrosis on plain radiographs and the AOFAS score correlated significantly with T2* values in the medial compartment of group 1 (p = 0.04 and 0.039, respectively). Biochemical, quantitative T2* MR mapping is likely effective to evaluate ankle arthrosis in cavovarus feet but further studies are required. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1562–1568, 2010
Modeling of chromatographic lipophilicity of food synthetic dyes estimated on different columns
Authors: Sârbu Costel , Casoni Dorina , Kot‐Wasik Agata , Wasik Andrzej , Namieśnik Jacek 
Published: June 2010

AbstractThe retention behavior of some food synthetic dyes has been studied by RP‐HPLC on chemically bonded C18, C8, C16 and CN stationary phases. Using methanol‐ammonium acetate (0.08 mol/L, pH=6.76) as mobile phase, a linear behavior of retention parameters throughout the methanol fraction variance was obtained in all cases (r>0.99). The patterns of chromatographic behavior of the compounds illustrate high similarities between the C18, C8 and C16 columns, respectively. Highly significant correlations were obtained between experimental lipophilicity indices log kw and φ0 estimated on C18 and C8 stationary phases and some computed log P‐values. An extensive investigation made for quantitative structure–property (lipophilicity) relationships of studied dyes, using descriptors from Dragon software, multiple linear regression and genetic algorithm, revealed that the molecular descriptors appearing in the best models combine 2‐D and 3‐D aspects of the molecular structure. The most significant descriptors can be classified as radial distribution function, GETAWAY (autocorrelation), 3D‐MoRSE signal, Burden eigenvalues and edge adjacency descriptors.
Liquid chromatography–tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients
Authors: Brozmanová Hana , Peřinová Ilona , Halvová Petra , Grundmann Milan 
Published: June 2010

AbstractA LC‐MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase 1 metabolites AM1, AM9, and AM4N in whole blood and lymphocytes isolated on the Histopaque gradient. 200 μL of whole blood was precipitated with 10 mol/L zinc sulfate in acetonitrile/methanol (40:60, v/v) and lymphocytes isolated from 1.5 mL blood were extracted with acetonitrile/methanol (40:60, v/v). The analytes and internal standard cyclosporine D were separated on RP column BEH C18, 2.1×50 mm, 1.7 μm using gradient LC‐MS/MS analysis in positive electrospray mode. Time of analysis was 5 min. Linearity in blood was 5–2000 μg/L for CsA, AM1, and AM9; 2–500 μg/L for AM4N; and 2–500 μg/L for all substances in lymphocytes. Coefficient of variations was 1.8–9.8% and recovery was 92.0–110.0%. The method was used in early and chronic renal transplant patients for therapeutic drug monitoring of CsA to compare either its share in lymphocytes as target organ or binding to one lymphocyte. The same parameters were calculated for all metabolites tested.
A simple validated GIS expert system to map relative soil vulnerability and patterns of erosion during the muddy floods of 2000–2001 on the South Downs, Sussex, UK
Authors: Faulkner H. , Boardman J. , Ruiz J.‐L. 
Published: June 2010

AbstractThe soils of the South Downs in East Sussex, England (UK), are dominated by loessic silt (>70 per cent) and are prone to crusting. Continuing erosion of these soils means that they are thin, typically less than 25 cm thick and are becoming stonier, more droughty and less easier to work. Rates of erosion are relatively low but during extreme events, soils are vulnerable and on‐ and off‐site erosion is a current and long‐term risk. Property damage due to muddy flooding is of particular concern. Due to a long history of research interest, a rich database exists on the erosional history of an area of approximately 75 km2of these thin, calcareous South Downs soils. In particular, during the winter of 2000–2001, Hortonian overland flow was common on certain crop types. Consequent sheet, rill and gully erosion was intense.The gullies and rills formed by runoff during these winter events were mapped in detail. In this paper, a method to estimate soil vulnerability to erosion is described and illustrated. Then, to validate the predictive efficacy of the algorithm used, the actual mapped distribution of rills and gullies following the winter events of 2001 on a particularly badly‐affected site are compared with predictions from our soil erosion vulnerability model. Methods for adjusting the land‐cover weightings to optimise the map fit are outlined.In a further survey of the utility of the map, it was discovered that farmers' recollections of events provided additional verification. Thus, one implication of our research is that erosion models can be validated by inviting farmers to comment on their efficacy to predict known histories. Copyright © 2010 John Wiley & Sons, Ltd.
Comparative corrosion studies of conventional nickel‐based brazing alloys and newly developed iron‐based brazing alloys
Authors: Wielage B. , Hoyer I. , Hausner S. 
Published: June 2010

AbstractIm vorliegenden Artikel werden neu entwickelte Lote auf Eisenbasis vorgestellt, die mit Liquidustemperaturen unterhalb von 1100°C als interessante und kostengünstige Alternative zu kommerziellen Nickelbasisloten angesehen werden können. Vergleichende Korrosionsuntersuchungen (Stromdichte‐Potenzial‐Untersuchungen) zeigen, dass die Eisenbasislote den Nickelbasisloten mindestens analoge, meist bessere Korrosionsbeständigkeiten aufweisen. Weiterhin werden Ergebnisse aus dem dynamischen Korrosionsprüfstand nach DIN EN 15664‐1 und damit erstmals Werte für die Nickelionenabgabe von Nickelbasisloten in Trinkwasser vorgestellt. Es zeigt sich, dass diese innerhalb der in der Trinkwasserverordnung vorgeschriebenen Grenzwerte liegen.This paper presents newly developed iron‐based brazing alloys, which are an interesting and a cost efficient alternative for commercial nickel‐based brazing alloys. Comparative corrosion studies (potentiodynamic corrosion test) show, that the iron‐based brazing alloys exhibit at least similar, but mostly better corrosion behaviour than the nickel‐based brazing alloys. Furthermore, the paper presents results from a dynamic corrosion test in conformity with DIN EN 15664‐1. The determined values show for the first time, that the release of nickel‐ions from nickel‐based brazing alloys is within the prescriptive limits of the drinking water regulation.