bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel easy-to-desorb eluant contributes to address environmental contamination of African swine fever virus
Authors: Zhang Li , Zhao Pengfei , Xia Yingjun , Hu Yanli , Wang Chaofei , Fang Rui , Zhao Junlong 
Published: May 2024

AbstractAfrican swine fever virus (ASFV) is a highly pathogenic and rapidly disseminated virus with strong viability in the environment, suggesting the importance of environmental detection for prevention and control in all the pig industry. However, the detection results of environmental swabs cannot always reflect the accurate status of viral pollution, leading to persistent ASFV environmental contamination. In this study, we developed an ASFV eluant with higher environmental ASFV detection efficiency relative to 0.85% saline solution, which obtains the patent certificate issued by the China Intellectual Property Office (patent number:202010976050.9). qPCR analysis showed that in the environmental swab samples, the number of viral copies was 100 times higher for the ASFV eluant treatment than the traditional eluant treatment (0.85% saline solution). And besides, the high sensitivity of the ASFV eluant had be verified in a slaughterhouse environmental sampling detection. In soil samples, the ASFV eluent showed the same extraction effect as the TIANamp Soil DNA Kit, in contrast to no extraction effect for 0.85% saline solution. Simultaneously, this eluent could protect ASFV from degradation and allow the transportation of samples at ambient temperature without refrigeration. In clinical practice, we monitored the environmental contamination condition of the ASFV in a large-scale pig farm. The results shown that the ASFV load decreased after every disinfection in environment. This study provides an effective solution for surveilling the potential threat of ASFV in environment.
Critical care beyond organ support: the importance of geriatric rehabilitation
Authors: Jacobs Jeremy M. , Rahamim Ana , Beil Michael , Guidet Bertrand , Vallet Helene , Flaatten Hans , Leaver Susannah K. , de Lange Dylan , Szczeklik Wojciech , Jung Christian , Sviri Sigal 
Published: May 2024

AbstractVery old critically ill patients pose a growing challenge for intensive care. Critical illness and the burden of treatment in the intensive care unit (ICU) can lead to a long-lasting decline of functional and cognitive abilities, especially in very old patients. Multi-complexity and increased vulnerability to stress in these patients may lead to new and worsening disabilities, requiring careful assessment, prevention and rehabilitation. The potential for rehabilitation, which is crucial for optimal functional outcomes, requires a systematic, multi-disciplinary approach and careful long-term planning during and following ICU care. We describe this process and provide recommendations and checklists for comprehensive and timely assessments in the context of transitioning patients from ICU to post-ICU and acute hospital care, and review the barriers to the provision of good functional outcomes.
Simple subspace based adaptive beamforming under Toeplitz covariances
Authors: Choi Yang-Ho 
Published: May 2024

AbstractWhen uncorrelated signals are incident on a uniform linear array, the array covariance matrix is of the Toeplitz form. An adaptive beamforming method is proposed based on the signal-plus-interference (SI) subspace via the Toeplitz rectification of the sample matrix. The rectified matrix is shown to be more accurate in a norm sense than the modified matrix according to the centro-Hermitian property. Since the former also is centro-Hermitian we can efficiently obtain its eigen-decomposition from a real matrix and then the weight vector in the estimated SI subspace. The proposed method, showing robustness to pointing errors, is not only computationally efficient but also very quickly converges to the optimum performance as demonstrated in the simulation.
Rinse, gel, and foam – is there any evidence for a difference in their effectiveness in preventing infections?
Authors: Boyce John M. , Pittet Didier 
Published: May 2024

Abstract
Background
Following publication of the 2009 World Health Organizations Guidelines for Hand Hygiene in Health Care, a debate has emerged regarding the relative antimicrobial efficacy of the different formats (rinse, gel, foam) of ABHRs and their ability to contribute to reduction of healthcare-associated infections (HAIs).

Methods
Data regarding the in-vivo antimicrobial efficacy of ABHRs and other factors that likely affect their effectiveness in reducing HAIs were reviewed, and a comprehensive review of studies that reported the effectiveness of each of the three ABHR formats to improve hand hygiene compliance and reduce HAIs was conducted.

Results
The amount of rubbing time it takes for hands to feel dry (dry time) is the major driver of ABHR antimicrobial efficacy. ABHR format is not a major factor, and several studies found that rinse, gel, and foam ABHRs have comparable in-vivo antimicrobial efficacy. Other factors that likely impact the ability of ABHRs to reduce transmission of healthcare-associated pathogens and HAIs include ABHR formulation, the volume applied to hands, aesthetic characteristics, skin tolerance, acceptance by healthcare personnel, and hand hygiene compliance rates. When accompanied by complementary strategies, promoting the use of each of the three ABHR formats has been associated with improvements in hand hygiene compliance rates. A review of 67 studies failed to identify an ABHR format that was significantly more effective in yielding statistically significant reductions in transmission of healthcare-associated pathogens or HAIs.

Conclusions
Current evidence is insufficient to definitively determine if one ABHR format is more effective in reducing transmission of healthcare-associated pathogens and HAIs. More rigorous studies such as multicenter randomized controlled trials comparing the different formats are needed to establish if one format is significantly more effective in reducing HAIs.
Comparative analysis of key factors influencing urban green space in Mashhad, Iran (1988–2018)
Authors: Rahmati Leila , Hanaei Toktam 
Published: May 2024

AbstractThis paper analyzed the role of national economic factors, in addition to some key city-level variables, in the variation of the urban green space (UGS) in Mashhad City (Iran) during three decades (1998–2018). The correlation result revealed the effects of the increasing trend of land price, population rate, and construction of built-up areas in the decreasing trend of UGS in the study area (R from − 0.95 to -0.99 at p-value > 95%). Also, the country-level economic factors, i.e., GDP per capita, oil price, export total value, and FDI, represented the overall increasing trend from 1988 to 2018, correlating with the decrease of green space areas (R from − 0.76 to -0.92 at p-value > 75%). Some statistical analyses, such as the run-test, the skewness and kurtosis tests, the Kolmogorov-Smirnov test, and the ANOVA test were done to confirm the normality of the data distribution and reliability of the results. Ultimately, clustering the research variables based on the significance and confidence levels of the estimated correlation results revealed that the change in the oil price and national export values in the petroleum-dependent economy of Iran can be assumed as the lead key economic factors to fluctuate all city-level variables, particularly the UGS variations.
Development, validation and application of single molecule molecular inversion probe based novel integrated genetic screening method for 29 common lysosomal storage disorders in India
Authors: Sheth Harsh , Nair Aadhira , Bhavsar Riddhi , Kamate Mahesh , Gowda Vykuntaraju K. , Bavdekar Ashish , Kadam Sandeep , Nampoothiri Sheela , Panigrahi Inusha , Kaur Anupriya , Shah Siddharth , Mehta Sanjeev , Jagadeesan Sujatha , Suresh Indrani , Kapoor Seema , Bajaj Shruti , Devi Radha Rama , Prajapati Ashka , Godbole Koumudi , Patel Harsh , Luhar Zulfiqar , Shah Raju C. , Iyer Anand , Bijarnia Sunita , Puri Ratna , Muranjan Mamta , Shah Ami , Magar Suvarna , Gupta Neerja , Tayade Naresh , Gandhi Ajit , Sowani Ajit , Kale Shrutikaa , Jalan Anil , Solanki Dhaval , Dalal Ashwin , Mane Shrikant , Prabha C. Ratna , Sheth Frenny , Joshi Chaitanya G. , Joshi Madhvi , Sheth Jayesh 
Published: May 2024

Abstract
Background
Current clinical diagnosis pathway for lysosomal storage disorders (LSDs) involves sequential biochemical enzymatic tests followed by DNA sequencing, which is iterative, has low diagnostic yield and is costly due to overlapping clinical presentations. Here, we describe a novel low-cost and high-throughput sequencing assay using single-molecule molecular inversion probes (smMIPs) to screen for causative single nucleotide variants (SNVs) and copy number variants (CNVs) in genes associated with 29 common LSDs in India.

Results
903 smMIPs were designed to target exon and exon–intron boundaries of targeted genes (n = 23; 53.7 kb of the human genome) and were equimolarly pooled to create a sequencing library. After extensive validation in a cohort of 50 patients, we screened 300 patients with either biochemical diagnosis (n = 187) or clinical suspicion (n = 113) of LSDs. A diagnostic yield of 83.4% was observed in patients with prior biochemical diagnosis of LSD. Furthermore, diagnostic yield of 73.9% (n = 54/73) was observed in patients with high clinical suspicion of LSD in contrast with 2.4% (n = 1/40) in patients with low clinical suspicion of LSD. In addition to detecting SNVs, the assay could detect single and multi-exon copy number variants with high confidence. Critically, Niemann-Pick disease type C and neuronal ceroid lipofuscinosis-6 diseases for which biochemical testing is unavailable, could be diagnosed using our assay. Lastly, we observed a non-inferior performance of the assay in DNA extracted from dried blood spots in comparison with whole blood.

Conclusion
We developed a flexible and scalable assay to reliably detect genetic causes of 29 common LSDs in India. The assay consolidates the detection of multiple variant types in multiple sample types while having improved diagnostic yield at same or lower cost compared to current clinical paradigm.
Validation of the Chinese version of the coping strategies for victims of cyberbullying scale
Authors: CHEN Qiqi , LU Zujian , LIU Bofan , XIAO Qiao , ZHU Yuhong , CHAN Ko Ling 
Published: May 2024

Abstract
Background
Although abundant evidence has confirmed cyberbullying as a global online risk, little is known about the coping strategies employed by victims and those who experiencing bullying. A validated scale for coping with cyberbullying could inform evidence-based social services and enable comparative studies of this phenomenon among victims from different backgrounds. This study aims to validate the Coping Strategies for Victims of Cyberbullying (CSVC) scale among Chinese adolescents and to compare its effectiveness between victims and bully-victims (individuals with dual roles).

Methods
A 25-item CSVC scale was translated and adapted for cultural relevance in the Chinese context. A sample of 1,716 adolescents, aged 13–18 years, from two middle schools and one high school in China, was recruited. Both exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were conducted.

Results
The EFA revealed that the Chinese version of the CSVC scale had satisfactory validity. The CFA demonstrated a good fit for the eight-factor model in assessing different coping strategies for cyberbullying. Differences in the selection of coping strategies were observed between the general adolescent population and sexual and gender minorities.

Conclusions
Future intervention studies may use this validated scale to educate adolescents, both those affected by cyberbullying and those who are not, to learn a broader range of coping strategies and to choose more effective ones.
Tsunami waveform inversion using Green’s functions with advection effects: application to the 2003 Tokachi–Oki earthquake
Authors: Yamanaka Yusuke , Tanioka Yuichiro 
Published: May 2024

AbstractWe explored nonlinear effects within the context of tsunami waveform inversion, wherein Green's functions were linearly superimposed to estimate earthquake slips. We focused on these effects while developing a source model for the 2003 Tokachi–Oki earthquake off Hokkaido, Japan. A source model for this earthquake was developed based on linear tsunami waveform inversion using Green’s functions and tsunami waveforms observed at tide gauge stations. Subsequently, tsunami waveforms from the source were simulated at the stations using nonlinear long-wave theory and compared with those estimated by inversion. The comparisons demonstrated that the waveforms had a non-negligible discrepancy that was attributed to advection effects, even for the primary wave used in the inversion at the two stations. This result strongly suggests that advection effects should be considered in the source modeling of the 2003 earthquake based on tsunami waveforms observed by tide gauges. Based on these results, a new tsunami waveform inversion technique that incorporates linearly approximated advection effects and maintain the framework of linear tsunami waveform inversion using Green’s functions is proposed and applied. The proposed method successfully mimicked the advection effects during the 2003 tsunami, reproduced better tsunami waveforms, and developed a source model for the 2003 earthquake using these effects. The peak slip amount and seismic moment were greater in the source model with advection effects than those without the effects. This finding suggests that the values in the source models developed for other earthquake events without considering these effects may have been underestimated.
Graphical abstract
Perineoplasty for anal incontinence after obstetric anal sphincter injury repair: a case report
Authors: Kojima Masatsugu , Miyake Toru , Tani Soichiro , Sakai Sachiko , Nishina Yusuke , Kaida Sachiko , Takebayashi Katsushi , Maehira Hiromitsu , Mori Haruki , Otake Reiko , Matsunaga Takashi , Ishikawa Hajime , Shimizu Tomoharu , Tani Masaji 
Published: May 2024

Abstract
Background
The rectal and vaginal walls are typically sutured if severe perineal lacerations with rectal mucosal damage occur during vaginal delivery. In case of anal incontinence after the repair, re-suturing of the anal sphincter muscle is standard procedure. However, this procedure may not result in sufficient improvement of function.

Case presentation
A 41-year-old woman underwent suture repair of the vaginal and rectal walls for fourth-degree perineal laceration at delivery. She was referred to our department after complaining of flatus and fecal incontinence. Her Wexner score was 15 points. Examination revealed decreased anal tonus and weak contractions on the ventral side. We diagnosed anal incontinence due to sphincter dysfunction after repair of a perineal laceration at delivery. We subsequently performed sphincter re-suturing with perineoplasty to restructure the perineal body by suturing the fascia located lateral to the perineal body and running in a ventral–dorsal direction, which filled the space between the anus and vagina and increased anal tonus. One month after surgery, the symptoms of anal incontinence disappeared (the Wexner score lowered to 0 points), and the anorectal manometry values increased compared to the preoperative values. According to recent reports on the anatomy of the female perineal region, bulbospongiosus muscle in women does not move toward the midline to attach to the perineal body, as has been previously believed. Instead, it attaches to the ipsilateral surface of the external anal sphincter. We consider the fascia lateral to the perineal body to be the fascia of the bulbospongiosus muscle.

Conclusions
In a case of postpartum anal incontinence due to sphincter dysfunction after repair of severe perineal laceration, perineoplasty with re-suturing an anal sphincter muscle resulted in improvement in anal sphincter function. Compared to conventional simple suture repair of the rectal wall only, this surgical technique may improve sphincter function to a greater degree.
The implications of the ecological footprint and renewable energy usage on the financial stability of South Asian countries
Authors: Imran Muhammad , Khan Muhammad Kamran , Alam Shabbir , Wahab Salman , Tufail Muhammad , Jijian Zhang 
Published: May 2024

AbstractThis study explores the complex relationships involving ecological footprints, energy use, carbon emissions, governance efficiency, economic prosperity, and financial stability in South Asian nations spanning the period from 2000 to 2022. Employing various methodologies such as cross-sectional dependence tests, co-integration analysis, and first- and second-generation unit-root tests, we use a panel Autoregressive Distributed Lag model, feasible generalized least squares, and Panel Corrected Standard Errors to ensure the robustness of our findings. We find noteworthy positive correlations between several variables, including heightened ecological consciousness, effective governance structures, increased GDP per capita, and amplified CO2 emissions. These relationships suggest potential pathways to strengthen the financial stability of the entire region; they also highlight the latent potential of embracing ecologically sustainable practices to fortify economic resilience. Our results also underscore the pivotal role of appropriate governance structures and higher income levels in bolstering financial stability in South Asian countries. Interestingly, we also find negative coefficients associated with the use of renewable energy, suggesting that escalating the adoption of renewable energy could create financial instability. This finding stresses the importance of diversification in energy strategies, cautioning policymakers to carefully consider the financial ramifications of potentially costly imports of renewable energy sources while seeking to reduce carbon emissions, emphasizing the need to strike a balance between ambitious sustainability goals and the pursuit of sustained economic robustness in the region. In considering the implications of these findings, it is crucial to consider each country’s broader socioeconomic context. Our results offer valuable insights for policymakers in developing renewable energy strategies.
“The patient is awake and we need to stay calm”: reconsidering indirect communication in the face of medical error and professionalism lapses
Authors: Taylor Taryn , Columbus Lauren , Banner Harrison , Seemann Natashia , Duncliffe Trevor Hines , Pack Rachael 
Published: May 2024

Abstract
Background
Although speaking up is lauded as a critical patient safety strategy, it remains exceptionally challenging for team members to enact. Existing efforts to address the problem of silence among interprofessional teams involve training low-authority members to use direct language and unambiguous challenge scripts. The role or value of indirect communication in preventing medical error remains largely unexplored despite its pervasiveness among interprofessional teams. This study explores the role of indirect challenges in the face of medical error and professionalism lapses.

Methods
Obstetricians at one academic center participated in an interprofessional simulation as a partial actor. Thirteen iterations were completed with 39 participants (13 obstetrician consultants, 11 obstetric residents, 2 family medicine consultants, 5 midwives, and 8 obstetrical nurses). Thirty participants completed a subsequent semi-structured interview. Five challenge moments were scripted for the obstetrician involving deliberate clinical judgment errors or professionalism infractions. Other participants were unaware of the obstetrician’s partial actor role. Scenarios were videotaped; debriefs and interviews were audio-recorded and transcribed verbatim and analyzed using a constructivist qualitative approach.

Results
Low-authority team members primarily relied on indirect challenge scripts to promote patient safety during simulation. Faculty participants were highly receptive to indirect challenges from low-authority team members, particularly in front of awake patients. In the context of obstetric care, direct challenges were actually viewed by participants as threatening to patient trust and disruptive to the interprofessional team. Instead of exclusively focusing our efforts on encouraging low-authority team members to speak up through direct challenges, it may be fruitful to expand our attention toward teaching faculty to identify, listen for, and respond to the indirect, subtle challenges that are already prolific among interprofessional teams.
Histological features of the gastrointestinal tract of elongate tigerfish, Hydrocynus forskahlii (Cuvier, 1819), from Lake Albert
Authors: Kasozi Nasser , Iwe Gerald D. , Langi Sandra , Namulawa Victoria T. , Walakira John 
Published: May 2024

Abstract
Background
The tigerfish (Hydrocynus forskahlii) is an important food fish in different regions of Africa. As such, interest in its performance and nutritional requirements as a potential candidate for aquaculture is increasing. Characterization of the morpho-histological features and functions of the gut provides valuable insights into the feeding physiology and digestive system of fish species.

Result
An investigation of the morpho-histological features of the gastrointestinal tract of H. forskahlii captured from Lake Albert was conducted over an eight-month period. The digestive tract of H. forskahlii is characterised by a short oesophagus, well-developed and distensible stomach and moderately long intestine. The oesophagus had a higher abundance of mucous-secreting goblet cells compared to other digestive tract tissues. The stomach had a bag-like shape for swallowing large prey and was distinguishable into the cardiac, fundic and pyloric regions. Both the cardiac and fundic regions of the stomach had numerous gastric glands, whereas the pyloric region had large compound folds consisting of pseudo-stratified columnar epithelium. Many goblet cells were also observed in the mid and posterior regions of the intestine. The relative length of the gut of the H. forskahlii was 1.27 ± 0.03 cm, and it had a total of 20–22 pyloric caeca. The liver was observed to be divided in lobules by a central vein contained within connective sheets. Irregular cords of hepatocytes were also visible throughout the parenchyma.

Conclusions
The histological features of the H. forskahlii gut observed in the present study are consistent with those of carnivorous and omnivorous fish.