bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salivary interleukin-17A and interleukin-18 levels in patients with celiac disease and periodontitis
Authors: Madi Marwa , Abdelsalam Maha , Elakel Ahmed , Zakaria Osama , AlGhamdi Maher , Alqahtani Mohammed , AlMuhaish Luba , Farooqi Faraz , Alamri Turki A. , Alhafid Ibrahim A. , Alzahrani Ibrahim M. , Alam Adel H. , Alhashmi Majed T. , Alasseri Ibrahim A. , AlQuorain Ahmad A. , AlQuorain Abdulaziz A. 
Published: May 2024

Background
An increased level of interleukin-17A and interleukin-18 in the serum and intestinal mucosa of celiac disease patients reflecting the severity of villous atrophy and inflammation was documented. Thus, the objective of this study was to evaluate the concentrations of salivary-17A, interleukin-1 beta, and interleukin-18 in patients with celiac disease who are on a gluten-free diet, both with and without periodontitis, and to compare these levels with those in healthy individuals.

Methods
The study involved 23 participants with serologically confirmed celiac disease (CD) and 23 control subjects. The CD patients had been following a gluten-free diet (GFD) for a minimum of 1 year and had no other autoimmune disorders. The research involved collecting demographic data, conducting periodontal examinations, gathering unstimulated whole saliva, and performing enzyme-linked immunosorbent assays to measure salivary interleukin-17A, interleukin-1 beta, and interleukin-18 levels. Spearman’s correlation analysis was utilized to explore the relationships between CD markers in patients on a GFD and their periodontal clinical findings.

Results
The periodontal findings indicated significantly lower values in celiac disease patients adhering to a gluten-free diet compared to control subjects (p = 0.001). No significant differences were found in salivary IL-17A, IL-18, and IL-1B levels between celiac disease patients and control subjects. Nevertheless, the levels of all interleukins were elevated in periodontitis patients in both the celiac and control groups. The IL-1 Beta level was significantly higher in periodontitis patients compared to non-periodontitis patients in the control group (p = 0.035). Significant negative correlations were observed between serum IgA levels and plaque index (r = −0.460, p = 0.010), as well as gingival index (r = −0.396, p = 0.030) in CD patients on a gluten-free diet.

Conclusion
Celiac disease patients on gluten-free diet exhibited better periodontal health compared to control subjects. However, increased levels of salivary IL-17A, IL-18 and IL-1B levels were associated with periodontitis. Additionally, serum IgA level was significantly inversely associated with periodontitis clinical manifestations and with salivary inflammatory mediators in CD patients on GFD.
Targeted Exon Sequencing by In‐Solution Hybrid Selection
Authors: Blumenstiel Brendan , Cibulskis Kristian , Fisher Sheila , DeFelice Matthew , Barry Andrew , Fennell Tim , Abreu Justin , Minie Brian , Costello Maura , Young Geneva , Maquire Jared , Kernytsky Andrew , Melnikov Alexandre , Rogov Peter , Gnirke Andreas , Gabriel Stacey 
Published: June 2010

AbstractThis unit describes a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in‐solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool are reviewed, as are critical steps for performing the preparation and hybrid selection of an Illumina paired‐end library. Critical parameters, performance metrics, and analysis workflow are discussed. Curr. Protoc. Hum. Genet. 66:18.4.1‐18.4.24 © 2010 by John Wiley & Sons, Inc.
Calcium Carbide
Authors: Cameron William 
Published: June 2010

AbstractChemically pure calcium carbide is a colorless solid. The pure material can be prepared only by special techniques. China is the world's largest producer of calcium carbide. Calcium carbide is produced commercially by the reaction of quicklime and a reducing agents such as coke in an electric furnace. The exothermic reaction of calcium carbide and water produces acetylene, which is the most important use of calcium carbide. Among the many uses of acetylene are as a cylinder gas for metal fabrication and construction, as an intermediate in the production of cosmetics, tabletted drugs, and antiseptics, and as a feedstock in the preparation of vinyl chloride, and polyurethane fibers. Calcium carbide is also used as a desulfurizing agent in steel making and for the manufacture of calcium cyanamide. Calcium carbide is considered a hazardous chemical under the U.S. Department of Transportation regulations.
Flavoring Materials
Authors: Fischetti Frank 
Published: June 2010

AbstractFlavor is viewed as a division between physical sense, ie, appearance, texture, and consistency and chemical sense, ie, taste, smell, and feeling. Flavors do several things in food systems. Foremost among these functions is to render food more acceptable and enjoyable. Flavor, nutritive value, mouthfeel, and appearance are the four features of food acceptance. The roles that taste and odor of flavorings are discussed. Flavors are divided into two main categories: natural and artificial. Natural materials include, eg, essential oils, spices, fruit, fruit juices, animal and vegetable extracts. Artificial flavors include aliphatic, aromatic, and terpene compounds that are made as opposed to being isolated from natural resources. These two categories are detailed as well as compounding and flavor formulations. Storage conditions could negatively affect flavoring materials. Flavor materials must be taste‐tested in the food itself. Safety is important in the manufacture of flavors and regulations regarding flavoring materials are discussed The incidence of allergy attributed to food flavor is low.
Ketenes, Ketene Dimers, and Related Substances
Authors: Taeschler Christoph 
Published: June 2010

AbstractThe parent ketene [463‐51‐4] and its dimer diketene [674‐82‐8] are of significant industrial importance, serving as starting material for many intermediates used mainly for the production of pigments, drugs and agrochemicals and intermediates for the paper industry. The most important intermediates consist of acetic anhydride, acetoacetic esters, amides and anilides. The use of ketene for the production of acetic anhydride has often been used for “recycling” acetic acid. Acetoacetic esters or diketene are utilized for the production of various heterocyclic intermediates. Long chain (C12 ‐ C20) Alkyl derivatives have been used as paper sizing agents.Ketenes typically possess a high reactivity, reacting readily with nucleophiles or undergoing [2+2] cycloadditions with double bonds as well as single bond insertions with polarized single bonds. The high reactivity is responsible for the limited stability under normal conditions; hence many ketenes exist merely as transient species. Diketene and its derivatives react with electrophilic and nucleophilic reagents, providing a broad spectrum of possibilities for possible products.
Molecular Imprinting
Authors: Spivak David 
Published: June 2010

AbstractMolecularly imprinted polymers (MIPs) are analytical materials that have widespread use for applications in the biomedical, analytical, chemical and biological sciences. Molecular imprinting is a process where a polymer, or other material, is formed around a template molecule. In this way, MIPs are tailored for specific recognition of the template molecule or related analogs and provide molecular recognition functions similar to antibodies and enzymes. Advantages of molecularly imprinted materials lie in the durability, relatively inexpensive cost, and ease of formation of network peptide polymers. This is in contrast to the problems of biomolecules that are unstable, generally expensive and often difficult and time consuming to obtain (usually from animal sources). Furthermore, the imprinted polymers are formed as network polymer solids that are predisposed for application as solid supports for HPLC, GC, sensors, and membranes. The robustness of the solid also affords stability in organic solvents, under harsh conditions such as heat or pressure, and gives MIPs a shelf life of many years. While the basic concepts of molecular imprinting are easy to understand, there are many important details that give further insight to obtaining the best performance in MIPs. This review covers many of these details and provides examples for greater insight into the method of molecular imprinting.
Antitumor effect of plant lectins
Authors: Abdullaev Fikrat I. , Gonzalez de Mejia Elvira 
Published: June 2010

AbstractThis review examines the literature data concerning the biological activity of plant lectins. Numerous studies have reported that these substances possess toxic, cytotoxic, antitumor, and anticarcinogenic properties. A brief description of the biological properties of plant lectins, as well as the effect of plant lectins on normal and malignant cells and the antitumor properties of these lectins in vivo and in vitro, are included. These findings are interpreted and possible mechanisms of the antitumor effect of plant lectins are discussed. Nat. Toxins 5:157–163, 1997. © 1997 Wiley‐Liss, Inc.
Aflatoxin exposure is higher in vegetarians than nonvegetarians in Thailand
Authors: Vinitketkumnuen Usanee , Chewonarin Teera , Kongtawelert Prachya , Lertjanyarak Apichai , Peerakhom Supatra , Wild Christopher P. 
Published: June 2010

AbstractAflatoxin‐albumin (AFB‐albumin) adducts and hepatitis B markers (anti‐HBs, and anti‐HBc) were measured in vegetarians and nonvegetarians from Chiang Mai, Thailand. The AFB‐albumin adduct levels were detected in 62% (37 of 60) of the vegetarian samples and 22% (22 of 100) of nonvegetarians. Somewhat higher levels were detected in vegetarians sera collected in the summer than in the winter, although this difference was not statistically significant. Subjects who were hepatitis B surface antigen (HBsAg)‐positive had slightly higher AFB‐albumin adduct levels than subjects who had evidence of past exposure (anti‐HBc‐positive) or no HB virus infection. This study indicated that vegetarians may have a higher frequency of aflatoxin exposure than nonvegetarians. Thai vegetarians consume various vegetables, grains, peanut, soybean, and fermented products, which have been reported to be sources of aflatoxin. Nat. Toxins 5:168–171, 1997. © 1997 Wiley‐Liss, Inc.
High‐performance liquid chromatographic separation of microcystins derivatized with a highly fluorescent dienophile
Authors: Harada Ken‐ichi , Oshikata Motoji , Shimada Takayuki , Nagata Akiko , Ishikawa Nami , Suzuki Makoto , Kondo Fumio , Shimizu Masato , Yamada Sachiko 
Published: June 2010

AbstractMicrocystins are potent hepatotoxins produced by cyanobacteria, and are also tumor promoters as well as potent inhibitors of the catalytic subunits of protein phosphatases 1 and 2A. In order to establish a physicochemical method for individual detection and determination of trace amounts of microcystins, we developed a derivatization method for fluorescence (FL) and chemiluminescence (CL) detection, in which a highly fluorescent dienophile, DMEQ‐TAD (4‐[2‐(6,7‐dimethoxy‐4‐methyl‐3‐oxo‐3,4‐dihydroquinoxalinyl) ethyl]‐1,2,4‐triazoline‐3,5‐dione), was used as the labeling reagent. DMEQ‐TAD reacted smoothly with the conjugated diene of the Adda moiety to give 2 stereoisomers of the adducts. As a result of the extensive experiments, the following reaction conditions were optimized for the labeling: sample amount, 10 μg; reaction solvent, DMF:acetonitrile (1:1); reaction time, 15 minutes; reaction temperature, 70°C; amount of DMEQ‐TAD used relative to that of microcystin, 80 equivalent. The resulting 6 adducts from microcystins‐LR, ‐YR, and ‐RR can be separated from one another using the following reversed phase HPLC conditions in combination with a clean‐up using ODS silica gel: column, Cosmosil 5C18‐AR (150 × 4.6 I.D. mm); mobile phase, methanol:0.05M phosphate buffer (pH 3) (1:1); flow rate, 1.0 ml/min; detection, FL λex 370 nm, λem 440 nm. The detection limits of the DMEQ‐TAD derivatives were estimated to be 100 and 500 pg for LR, and 65 and 2,500 pg for RR using FL and CL detections, respectively; and the detection behavior was different from that of the Dns‐Cys derivatives, which were more sensitive to CL than FL. Nat. Toxins 5:201–207, 1997. © 1998 Wiley‐Liss, Inc.
The Paediatric Rheumatology International Trials Organisation provisional criteria for the evaluation of response to therapy in juvenile dermatomyositis
Authors: Ruperto Nicolino , Pistorio Angela , Ravelli Angelo , Rider Lisa G. , Pilkington Clarissa , Oliveira Sheila , Wulffraat Nico , Espada Graciela , Garay Stella , Cuttica Ruben , Hofer Michael , Quartier Pierre , Melo‐Gomes Jose , Reed Ann M. , Wierzbowska Malgorzata , Feldman Brian M. , Harjacek Miroslav , Huppertz Hans‐Iko , Nielsen Susan , Flato Berit , Lahdenne Pekka , Michels Harmut , Murray Kevin J. , Punaro Lynn , Rennebohm Robert , Russo Ricardo , Balogh Zsolt , Rooney Madeleine , Pachman Lauren M. , Wallace Carol , Hashkes Philip , Lovell Daniel J. , Giannini Edward H. , Gare Boel Andersson , Martini Alberto 
Published: June 2010

AbstractObjectiveTo develop a provisional definition for the evaluation of response to therapy in juvenile dermatomyositis (DM) based on the Paediatric Rheumatology International Trials Organisation juvenile DM core set of variables.MethodsThirty‐seven experienced pediatric rheumatologists from 27 countries achieved consensus on 128 difficult patient profiles as clinically improved or not improved using a stepwise approach (patient's rating, statistical analysis, definition selection). Using the physicians' consensus ratings as the “gold standard measure,” chi‐square, sensitivity, specificity, false‐positive and‐negative rates, area under the receiver operating characteristic curve, and kappa agreement for candidate definitions of improvement were calculated. Definitions with kappa values >0.8 were multiplied by the face validity score to select the top definitions.ResultsThe top definition of improvement was at least 20% improvement from baseline in 3 of 6 core set variables with no more than 1 of the remaining worsening by more than 30%, which cannot be muscle strength. The second‐highest scoring definition was at least 20% improvement from baseline in 3 of 6 core set variables with no more than 2 of the remaining worsening by more than 25%, which cannot be muscle strength (definition P1 selected by the International Myositis Assessment and Clinical Studies group). The third is similar to the second with the maximum amount of worsening set to 30%. This indicates convergent validity of the process.ConclusionWe propose a provisional data‐driven definition of improvement that reflects well the consensus rating of experienced clinicians, which incorporates clinically meaningful change in core set variables in a composite end point for the evaluation of global response to therapy in juvenile DM.
Observational study to determine predictors of rheumatology clinic visit provider contact time
Authors: Davis Lisa A. , Larson Molly M. , Caplan Liron 
Published: June 2010

AbstractObjectiveTo address perceived inefficiencies in an academic rheumatology practice, a timing/work‐flow evaluation was initiated to determine the factors that predict the provider contact time (PCT), i.e., the amount of time that attending physicians spend with patients during an outpatient encounter.MethodsThis prospective observational study was conducted at the University of Colorado Hospital Rheumatology Clinic for return patient visits in early 2008. Each patient encounter was subdivided into components, and the time for each component was recorded. Up to 20 return‐visit encounters per provider were randomly selected for inclusion. Multivariate linear regression was used to predict the time, in minutes, that providers spent with patients, and logistic regression was used to determine the time intervals associated with the patient's perception that the visit ran on time.ResultsVariables associated with increased PCT were whether a procedure was performed in the clinic (P = 0.037) and whether the visit occurred in the afternoon (P < 0.025). For every minute a provider was late in beginning to see a patient, the PCT decreased by 0.32 minutes (95% confidence interval [95% CI] 0.15, 0.49). Variables associated with the patient's perception that the visit ran on time included the check‐in to vitals delay (odds ratio [OR] 0.95; 95% CI 0.92, 0.99) and the provider delay (OR 0.92; 95% CI 0.86, 0.99).ConclusionThe patient's punctuality and the presence of a resident are not significantly associated with the time that a provider spends with a patient. However, the degree to which the provider runs late was associated with decreased PCT and diminishes the patient's perception that the visit is running on time.
Maternal smoking during pregnancy is associated with epigenetic modifications of the brain‐derived neurotrophic factor‐6 exon in adolescent offspring
Authors: Toledo‐Rodriguez Maria , Lotfipour Shahrdad , Leonard Gabriel , Perron Michel , Richer Louis , Veillette Suzanne , Pausova Zdenka , Paus Tomáš 
Published: June 2010

AbstractPrenatal exposure to maternal cigarette smoking (PEMCS) is associated with variations in brain and behavior in adolescence. Epigenetic mechanisms may mediate some of the consequences of PEMCS through methylation of deoxyribonucleic acid (DNA) in genes important for brain development, such as the brain‐derived neurotrophic factor (BDNF). In the current study, we used bisulfite sequencing to assess DNA methylation of the BDNF promoter in the blood of adolescents whose mothers smoked during pregnancy. We demonstrate that PEMCS is associated with higher rates of DNA methylation in the BDNF‐6 exon. These results suggest that PEMCS may lead to long‐term down‐regulation of BDNF expression via the increase of DNA methylation in its promoter region. Such mechanisms could, in turn, lead to modifications in both development and plasticity of the brain exposed in utero to maternal cigarette smoking. © 2010 Wiley‐Liss, Inc.
Chronic cerebrospinal venous insufficiency and multiple sclerosis
Authors: Khan Omar , Filippi Massimo , Freedman Mark S. , Barkhof Frederik , Dore‐Duffy Paula , Lassmann Hans , Trapp Bruce , Bar‐Or Amit , Zak Imad , Siegel Marilyn J. , Lisak Robert 
Published: June 2010

AbstractA chronic state of impaired venous drainage from the central nervous system, termed chronic cerebrospinal venous insufficiency (CCSVI), is claimed to be a pathologic phenomenon exclusively seen in multiple sclerosis (MS). This has invigorated the causal debate of MS and generated immense interest in the patient and scientific communities. A potential shift in the treatment paradigm of MS involving endovascular balloon angioplasty or venous stent placement has been proposed as well as conducted in small patient series. In some cases, it may have resulted in serious injury. In this Point of View, we discuss the recent investigations that led to the description of CCSVI as well as the conceptual and technical shortcomings that challenge the potential relationship of this phenomenon to MS. The need for conducting carefully designed and rigorously controlled studies to investigate CCVSI has been recognized by the scientific bodies engaged in MS research. Several scientific endeavors examining the presence of CCSVI in MS are being undertaken. At present, invasive and potentially dangerous endovascular procedures as therapy for patients with MS should be discouraged until such studies have been completed, analyzed, and debated in the scientific arena. ANN NEUROL 2010;67:286–290
Zur Chemie der Acylcyanide
Authors: Hünig Siegfried , Schaller Rainer 
Published: June 2010

AbstractDurch verbesserte und neue Synthesen sind Acylcyanide, vor allem auch aliphatische Acylcyanide, so gut zugänglich geworden, daß sie sich für zahlreiche Anwendungen anbieten. Diese Zusammenfassung berichtet über die Entwicklung der Chemie der Acylcyanide während der letzten 25 Jahre. In vier großen Formelschemata sind neben Synthesen für RCOCN ihre wichtigsten Reaktionsmöglichkeiten zusammengestellt: Umsetzungen an der CO‐ und der CN‐Gruppe — jeweils unter Erhaltung des Kohlenstoffgerüstes — sowie Acylierungen unter Abspaltung von Cyanid‐Ionen.
Das bakterielle Ribosom: ein programmierbares Enzym
Authors: Gassen Hans Günter 
Published: June 2010

AbstractDie Ribosomen‐abhängige Proteinbiosynthese in Bakterien ist ein komplexer Vorgang, an dem über 150 Komponenten beteiligt sind. Das Ribosom, das aus 53 Proteinen und drei Nucleinsäuren aufgebaut ist, katalysiert als Enzym die Bildung der Peptidbindung. Anders jedoch als konventionelle Enzyme ist das Ribosom durch die mRNA — das Transkript der DNA — „programmierbar”︁. Es entziffert die Nucleotidsequenz der mRNA und setzt sie in die Aminosäuresequenz eines Proteins um. Ähnlich wie bei der Muskelkontraktion wird hierbei chemische Bindungsenergie benutzt, um eine mechanische Bewegung zu ermöglichen. Das hier vorgestellte Modell der Proteinbiosynthese an programmierbaren Ribosomen gibt Antworten auf folgende Fragen: Wie wird die mRNA in Informationseinheiten gerastert, wie wird die Substratspezifität des Ribosoms durch das Codon moduliert und wie bewegt sich die mRNA am Ribosom um ein Trinucleotid pro gebildete Peptidbindung vorwärts?
Wie ähnlich sind sich Tropochinone und Benzochinone?
Authors: Gleiter Rolf , Dobler Walter , Eckert‐Maksić Mirjana 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 13. DOI: 10.1002/ange.198200130
Palladacycloalkane durch oxidative Kupplung von 3,3‐Dimethylcyclopropen mit Palladium(0)‐Verbindungen
Authors: Binger Paul , Michaelbüch Holger , Benn Reinhard , Mynott Richard 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 153. DOI: 10.1002/ange.198201530
Thermische Umwandlung von 1,3‐Cyclooctadien‐5‐in in Benzocyclobuten — eine neue Isomerisierung auf der C8H8‐Hyperfläche
Authors: Meier Herbert , Hanold Norbert , Kolshorn Heinz 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 85. DOI: 10.1002/ange.198200850
1,3‐Cyclooctadien‐6‐in — ein isolierbares Bishomodehydrobenzol
Authors: Meier Herbert , Echter Toni 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 91. DOI: 10.1002/ange.198200910
Ein Ethylen(hydrido)wolfram‐Komplex durch photoinduzierte β‐Eliminierung
Authors: Alt Helmut Guido , Eichner Manfred Erwin 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 121. DOI: 10.1002/ange.198201210
Stereokontrollierte Synthese von (+)‐Sarracenin durch photochemische Cycloaddition
Authors: Tietze Lutz‐F. , Glüsenkamp Karl‐Heinz , Nakane Masami , Hutchinson C. Richard 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 126. DOI: 10.1002/ange.198201260
Verbrückte C5Me5CoII‐Komplexe ‐ reaktive Zwischenstufen der Cyclopentadienylierung von Cobalt(II)‐halogeniden
Authors: Kölle Ulrich , Khouzami Fouad , Fuss Bernhard 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 230. DOI: 10.1002/ange.198202300
Wasserstoffentwicklung aus photochemisch reduzierten, wasserlöslichen Zinn(IV)‐porphyrinen
Authors: Krüger Wolfgang , Fuhrhop Jürgen‐Hinrich 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 193. DOI: 10.1002/ange.198201930
Umpolung der Carbonylreaktivität über Acetoxyalkyl‐Radikale
Authors: Giese Bernd , Erfort Ulrich 
Published: June 2010

AbstractDas vollständige Manuskript dieser Zuschrift erscheint in: Angew. Chem. Suppl. 1982, 250. DOI: 10.1002/ange.198202500