bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of the time of diagnosis on outcome in patients with acute venous thromboembolism
Authors: Lecumberri Ramón , Soler Silvia , Toro Jorge Del , Barba Raquel , Rosa Vladimir , Ciammaichella Maurizio , Monreal Manuel ,   the RIETE Investigators
Published: October 2010

SummaryThe influence of the day of diagnosis (weekends vs. weekdays) on outcome in patients with acute venous thromboembolism (VTE) has not been thoroughly studied. We used the RIETE database to compare the clinical characteristics, treatment details, and mortality rate at 7 and 30 days, of all patients diagnosed with acute VTE on weekends versus those diagnosed on weekdays. Up to January 2010, 30,394 patients were included in RIETE, of whom 5,479 (18%) were diagnosed on weekends. Most clinical characteristics were similar in both groups, but patients diagnosed on weekends had less often cancer (20% vs. 22%; p=0.004), and presented more likely with pulmonary embolism (PE) than those diagnosed on weekdays (52% vs. 47%; p <0.001). Most patients in both groups received initial therapy with low-molecular-weight heparin (90% and 91%, respectively; p=0.01), then switched to vitamin K antagonists (72% and 71%, respectively; p=0.007). The 7-day mortality rate in patients presenting with PE was 2.75% in those diagnosed on weekends versus 3.00% in those diagnosed on weekdays (p=0.49). At 30 days, the mortality rate was 6.51% versus 6.06%, respectively (p=0.38). In patients presenting with deep vein thrombosis alone, the 7-day mortality rate in those diagnosed on weekends was 1.04% versuss 0.66% in those diagnosed on weekdays (p=0.053). The mortality rate at 30 days was of 3.41% versus 2.88% (p=0.14), respectively. In RIETE, the clinical characteristics, treatment strategies, and 7– and 30-day mortality rates of patients diagnosed on weekends were similar to those in patients diagnosed on weekdays.
A Study on the Feasibility of Dual-Wavelength Radar for Identification of Hydrometeor Phases
Authors: Liao Liang , Meneghini Robert 
Published: October 2010

Abstract
An important objective for the dual-wavelength Ku-/Ka-band precipitation radar (DPR) that will be on board the Global Precipitation Measurement (GPM) core satellite is to identify the phase state of hydrometeors along the range direction. To assess this, radar signatures are simulated in snow and rain to explore the relation between the differential frequency ratio (DFR), defined as the difference of radar reflectivity factors between Ku and Ka bands, and the radar reflectivity factor at Ku band ZKu for different hydrometeor types. Model simulations indicate that there is clear separation between snow and rain in the ZKu–DFR plane assuming that the snow follows the Gunn–Marshall size distribution and rain follows the Marshall–Palmer size distribution. In an effort to verify the simulated results, the data collected by the Airborne Second-Generation Precipitation Radar (APR-2) in the Wakasa Bay Advanced Microwave Scanning Radiometer for Earth Observing System (AMSR-E) campaign are employed. Using the signatures of linear depolarization ratio at Ku band, the APR-2 data can be easily divided into the regions of snow, mixed phase, and rain for stratiform storms. These results are then superimposed onto the theoretical curves computed from the model in the ZKu–DFR plane. For over 90% of the observations from a cold-season stratiform precipitation event, snow and rain can be distinguished if the Ku-band radar reflectivity exceeds 18 dBZ (the minimum detectable level of the GPM DPR at Ku band). This is also the case for snow and mixed-phase hydrometeors. Although snow can be easily distinguished from rain and melting hydrometeors by using Ku- and Ka-band radar, the rain and mixed-phase particles are not always separable. It is concluded that Ku- and Ka-band dual-wavelength radar might provide a potential means to identify the phase state of hydrometeors.
Systemic cytokine response in patients with community-acquired pneumonia
Authors: Endeman H. , Meijvis S.C.A. , Rijkers G.T. , van Velzen–Blad H. , van Moorsel C.H.M. , Grutters J.C. , Biesma D.H. 
Published: October 2010

The role of individual cytokines and polymorphisms in pneumonia has been described, but the relationship between different cytokines and polymorphisms in relation to causative microorganisms, antibiotics, corticosteroids and clinical course has not. This study questions the relationship between cytokines, polymorphisms and clinical characteristics of pneumonia.Patients diagnosed with pneumonia were included in the study. Serum cytokine levels were measured during hospital stay, genotyping was performed, causative microorganisms were identified and patients were monitored throughout the hospital stay.In 201 patients with pneumonia interleukin (IL)-1 receptor antagonist (IL-1RA), IL-6, IL-8 and IL-10 acted as acute phase proteins. After admission, the levels of these cytokines decreased rapidly. Single nucleotide polymorphisms did not influence cytokine production and were not associated with clinical outcome. Cytokine serum levels were significantly higher in patients with pneumococcal pneumonia. The decrease in levels of cytokines was independently influenced by the start of corticosteroid therapy.IL-1RA, IL-6, IL-8 and IL-10 are acute phase proteins, independent of genotype. Their levels are influenced by the nature of the causative microorganism and the start of corticosteroids therapy.
Scoring of senescence signalling in multiple human tumour gene expression datasets, identification of a correlation between senescence score and drug toxicity in the NCI60 panel and a pro-inflammatory signature correlating with survival advantage in peritoneal mesothelioma
Authors: Lafferty-Whyte Kyle , Bilsland Alan , Cairney Claire J , Hanley Lorna , Jamieson Nigel B , Zaffaroni Nadia , Oien Karin A , Burns Sharon , Roffey Jon , Boyd Susan M , Keith W Nicol 
Published: October 2010

Abstract

Background
Cellular senescence is a major barrier to tumour progression, though its role in pathogenesis of cancer and other diseases is poorly understood in vivo. Improved understanding of the degree to which latent senescence signalling persists in tumours might identify intervention strategies to provoke "accelerated senescence" responses as a therapeutic outcome. Senescence involves convergence of multiple pathways and requires ongoing dynamic signalling throughout its establishment and maintenance. Recent discovery of several new markers allows for an expression profiling approach to study specific senescence phenotypes in relevant tissue samples. We adopted a "senescence scoring" methodology based on expression profiles of multiple senescence markers to examine the degree to which signals of damage-associated or secretory senescence persist in various human tumours.

Results
We first show that scoring captures differential induction of damage or inflammatory pathways in a series of public datasets involving radiotherapy of colon adenocarcinoma, chemotherapy of breast cancer cells, replicative senescence of mesenchymal stem cells, and progression of melanoma. We extended these results to investigate correlations between senescence score and growth inhibition in response to ~1500 compounds in the NCI60 panel. Scoring of our own mesenchymal tumour dataset highlighted differential expression of secretory signalling pathways between distinct subgroups of MPNST, liposarcomas and peritoneal mesothelioma. Furthermore, a pro-inflammatory signature yielded by hierarchical clustering of secretory markers showed prognostic significance in mesothelioma.

Conclusions
We find that "senescence scoring" accurately reports senescence signalling in a variety of situations where senescence would be expected to occur and highlights differential expression of damage associated and secretory senescence pathways in a context-dependent manner.
TNFRSF11B computational development network construction and analysis between frontal cortex of HIV encephalitis (HIVE) and HIVE-control patients
Authors: Huang Ju X , Wang L , Jiang Ming H 
Published: September 2010

Abstract

Background

TNFRSF11B computational development network construction and analysis of frontal cortex of HIV encephalitis (HIVE) is very useful to identify novel markers and potential targets for prognosis and therapy.

Methods
By integration of gene regulatory network infer (GRNInfer) and the database for annotation, visualization and integrated discovery (DAVID) we identified and constructed significant molecule TNFRSF11B development network from 12 frontal cortex of HIVE-control patients and 16 HIVE in the same GEO Dataset GDS1726.

Results
Our result verified TNFRSF11B developmental process only in the downstream of frontal cortex of HIVE-control patients (BST2, DGKG, GAS1, PDCD4, TGFBR3, VEZF1 inhibition), whereas in the upstream of frontal cortex of HIVE (DGKG, PDCD4 activation) and downstream (CFDP1, DGKG, GAS1, PAX6 activation; BST2, PDCD4, TGFBR3, VEZF1 inhibition). Importantly, we datamined that TNFRSF11B development cluster of HIVE is involved in T-cell mediated immunity, cell projection organization and cell motion (only in HIVE terms) without apoptosis, plasma membrane and kinase activity (only in HIVE-control patients terms), the condition is vital to inflammation, brain morphology and cognition impairment of HIVE. Our result demonstrated that common terms in both HIVE-control patients and HIVE include developmental process, signal transduction, negative regulation of cell proliferation, RNA-binding, zinc-finger, cell development, positive regulation of biological process and cell differentiation.

Conclusions
We deduced the stronger TNFRSF11B development network in HIVE consistent with our number computation. It would be necessary of the stronger TNFRSF11B development function to inflammation, brain morphology and cognition of HIVE.
Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain (D190A) that occurred upon virus transmission from turkeys to pigs
Authors: Yassine Hadi M , Khatri Mahesh , Lee Chang W , Saif Yehia M 
Published: September 2010

Abstract
The hemagglutinin (HA) protein of influenza virus mediates essential viral functions including the binding to host receptor and virus entry. It also has the antigenic sites required for virus neutralization by host antibodies. Here, we characterized an H3N2 triple reassortant (TR) influenza virus (A/turkey/Ohio/313053/04) with a mutation at the receptor binding domain (Asp190Ala) that occurred upon virus transmission from turkeys to pigs in an experimental infection study. The mutant virus replicated less efficiently than the parental virus in human, pig and turkey primary tracheal/bronchial epithelial cells, with more than 3-log10 difference in virus titer at 72 hours post infection. In addition, the mutant virus demonstrated lower binding efficiency to plasma membrane preparations from all three cell types compared to the parental virus. Antisera raised against the parental virus reacted equally to both homologous and heterlogous viruses, however, antisera raised against the mutant virus showed 4-8 folds lower reactivity to the parental virus.
Beyond allostery: Catalytic regulation of a deoxyribozyme through an entropy-driven DNA amplifier
Authors: Eckhoff Grace , Codrea Vlad , Ellington Andrew D , Chen Xi 
Published: October 2010

Abstract
The programmability and replicability of RNA and DNA have respectively enabled the design and selection of a number of allosteric ribozymes and deoxyribozymes. These catalysts have been adapted to function as signal transducers in biosensors and biochemical reaction networks both in vitro and in vivo. However, allosteric control of nucleic acid catalysts is currently limited by the fact that one molecule of effector (input) generally regulates at most one molecule of ribozyme or deoxyribozyme (output). In consequence, allosteric control is usually inefficient when the concentration of input molecules is low. In contrast, catalytic regulation of protein enzymes, as in protein phosphorylation cascades, generally allows one input molecule (e.g., one kinase molecule) to regulate multiple output molecules (e.g., kinase substrates). Achieving such catalytic signal amplification would also be of great utility for nucleic acid circuits. Here we show that allosteric regulation of nucleic acid enzymes can be coupled to signal amplification in an entropy-driven DNA circuit. In this circuit, kinetically trapped DNA logic gates are triggered by a specific sequence, and upon execution generate a peroxidase deoxyribozyme that converts a colorless substrate (ABTS) into a green product (ABTS•+). This scheme provides a new paradigm for the design of enzyme-free biosensors for point-of-care diagnostics.
Robust erythrophagocytosis leads to macrophage apoptosis via a hemin-mediated redox imbalance: role in hemolytic disorders
Authors: Cambos Mathieu , Scorza Tatiana 
Published: October 2010

ABSTRACTMP from the RES are responsible for the clearance of senescent RBC. Although the frequency of senescent RBC is low under steady-state conditions, it increases dramatically during hemolytic disorders, resulting in enhanced erythrophagocytosis. As erythrophagocytosis has been involved in MP dysfunction and as certain hemolytic disorders associate to MP apoptosis, a possible link between erythrophagocytosis and the viability of phagocytes was investigated herein. To mimic hemolytic disorders, two distinct in vitro models, artificially oxidized RBC and DSRBC, were chosen to study the erythrophagocytosis impact on the viability of J774A.1 MP. Although CRBC were weakly phagocytosed and did not affect MP viability significantly, erythrophagocytosis of oxidized RBC and DSRBC was robust and resulted in a sharp decrease of MP viability via apoptosis. Under these conditions, Hb-derived HE was shown to be involved in the induction of apoptosis. Moreover, oxidized RBC, DSRBC, and HE generated ROS species, which were responsible for the apoptosis of MP. Furthermore, HO-1, strongly induced in response to treatment with oxidized RBC, DSRBC, or HE, was shown to protect MP partially against apoptosis, suggesting that robust erythro-phagocytosis may exceed the detoxification capabilities of MP. Taken together, these results suggest that enhanced erythrophagocytosis associated to hemolytic disorders leads to MP apoptosis in vitro and may have critical implications for the control of malaria infection and for the exacerbated susceptibility to bacterial infections during hemolytic disorders.
Cost-effectiveness of improved primary care treatment of depression in women in Chile
Authors: Siskind Dan , Araya Ricardo , Kim Jane 
Published: September 2010

BackgroundLow- and middle-income countries lack information on contextualised mental health interventions to aid resource allocation decisions regarding healthcare.AimsTo undertake a cost-effectiveness analysis of treatments for depression contextualised to Chile.MethodsUsing data from studies in Chile, we developed a computer-based Markov cohort model of depression among Chilean women to evaluate the cost-effectiveness of usual care or improved stepped care.ResultsThe incremental cost-effectiveness ratio (ICER) of usual care was I$113 per quality-adjusted life-year (QALY) gained, versus no treatment, whereas stepped care had an ICER of I$468 per QALY versus usual care. This compared favourably with Chile's per-capita GDP. Results were most sensitive to variation in recurrent episode coverage, marginally sensitive to cost of treatment, and insensitive to changes in health-state utility of depression and rate of recurrence.ConclusionsOur results suggest that treatments for depression in lowand middle-income countries may be more cost-effective than previously estimated.
Evaluation of antipsychotic information on UK schizophrenia websites
Authors: Akram Gazala , Boyter Anne , Thomson Alison 
Published: September 2010

Aims and methodTo evaluate the quality of information about medication on 33 UK schizophrenia websites using a bespoke tool known as the Strathclyde Website Evaluation Form (SWEF).ResultsThe mean total SWEF score was 26 out of a maximum of 52. For website content, the mean score was 15.3 out of a maximum of 32. Most websites mentioned antipsychotics (n = 23), but only 10 described the differences between typical and atypical classes. Three websites mentioned the different injectable formulations. Information about side-effects, particularly from atypicals was limited. Comprehensive details about clozapine including the blood monitoring requirements were found on two websites. Currency was reasonable with 20 websites being less than 3 years old.Clinical implicationsInformation about antipsychotic medication on UK schizophrenia websites is basic and incomplete.
Commentary: The Year in Nuclear Receptor Control of Metabolism
Authors: Mangelsdorf David J. 
Published: September 2010

Abstract
This commentary highlights some of the most important discoveries in the field of nuclear receptor control of metabolism that occurred over the past year (2009 to 2010). As might be expected in a field that encompasses several hundred active laboratories, the task of providing a balanced look at these discoveries was daunting. Thus, to help make the selection of these discoveries, a small panel of colleagues was recruited to help. After selecting the top candidate discoveries from a Google search and a PubMed search, the panel was asked to rank them. These final selections were presented at “The Year In Basic Science Session” of the Annual Meeting of the Endocrine Society, ENDO 2010, the highlights of which are reproduced in this article.
Wake structure and wing kinematics: the flight of the lesser dog-faced fruit bat,Cynopterus brachyotis
Authors: Hubel Tatjana Y. , Riskin Daniel K. , Swartz Sharon M. , Breuer Kenneth S. 
Published: October 2010

SUMMARYWe investigated the detailed kinematics and wake structure of lesser dog-faced fruit bats (Cynopterus brachyotis) flying in a wind tunnel. High speed recordings of the kinematics were conducted to obtain three-dimensional reconstructions of wing movements. Simultaneously, the flow structure in the spanwise plane perpendicular to the flow stream was visualized using time-resolved particle image velocimetry. The flight of four individuals was investigated to reveal patterns in kinematics and wake structure typical for lower and higher speeds. The wake structure identified as typical for both speed categories was a closed-loop ring vortex consisting of the tip vortex and the limited appearance of a counter-rotating vortex near the body, as well as a small distally located vortex system at the end of the upstroke that generated negative lift. We also investigated the degree of consistency within trials and looked at individual variation in flight parameters, and found distinct differences between individuals as well as within individuals.
The shadow-induced withdrawal response, dermal photoreceptors, and their input to the higher-order interneuron RPeD11 in the pond snailLymnaea stagnalis
Authors: Sunada Hiroshi , Sakaguchi Taichi , Horikoshi Tetsuro , Lukowiak Ken , Sakakibara Manabu 
Published: October 2010

SUMMARYThe shadow-induced withdrawal response in Lymnaea stagnalis is mediated by dermal photoreceptors located on the foot, mantle cavity, and skin around the pneumostome area. Here, we determined whether we could obtain a neural correlate of the withdrawal response elicited by a shadow in a higher-order central neuron that mediates withdrawal behavior. We measured the electrophysiological properties of the higher-order interneuron Right Pedal Dorsal 11 (RPeD11), which has a major role in Lymnaea withdrawal behavior. In semi-intact preparations comprising the circumesophageal ganglia, the mantle cavity and the pneumostome, but not the foot and eyes, a light-on stimulus elicited a small short-lasting hyperpolarization and a light-off stimulus elicited a depolarization of RPeD11. We also determined that dermal photoreceptors make a monosynaptic contact with RPeD11. The dermal photoreceptor afferents course to the circumesophageal ganglia via the anal and genital nerves to the visceral ganglion, and/or via the right internal and external parietal nerves to the parietal ganglion. Finally, in addition to responding to photic stimuli, RPeD11 responds to both mechanical and chemical stimuli delivered to the pneumostome.
Sexually dimorphic sensory gating drives behavioral differences in túngara frogs
Authors: Hoke Kim L. , Ryan Michael J. , Wilczynski Walter 
Published: October 2010

SUMMARY
Males and females can differ both in the social behaviors they perform and in the contexts in which they engage in these behaviors. One possible mechanism of sex differences in behavior is a sexual dimorphism in the relay of sensory information to motor areas, but no studies have examined the role of such a relay in vertebrate sexually dimorphic behaviors. We used egr-1 expression as a marker of neural activation in frogs exposed to conspecific and heterospecific acoustic signals to compare activation patterns throughout the brains of males and females. We determined how the sexes differ in the transformation of social signals into motor responses in the context of social communication. We examined the relationships between egr-1 mRNA levels in the auditory midbrain and forebrain areas, as well as how forebrain expression related to the behavioral responses of the animals. Forebrain network activation patterns and forebrain–behavior relationships were similar in males and females. By contrast, we found a sex difference in the relationship between midbrain and forebrain activation; midbrain auditory responses predicted forebrain responses in females but not in males. This sex difference suggests that sensory inputs differentially regulate motor systems underlying social behaviors in males and females. This sensorimotor transformation may be a common locus for generating sex differences in behavior.
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue
Authors: Yamada Akira , Tamori Masaki , Iketani Tomoaki , Oiwa Kazuhiro , Motokawa Tatsuo 
Published: October 2010

SUMMARYThe dermis of sea cucumbers is a catch connective tissue or mutable collagenous tissue that shows large changes in stiffness. Extensive studies on the dermis revealed that it can adopt three different states having different mechanical properties that can be reversibly converted. These are the stiff, standard and soft states. The standard state is readily produced when a dermal piece is immersed in the sea water containing Ca2+, whereas the soft state can be produced by removal of Ca2+. A stiffening protein, tensilin, has been isolated from some sea cucumbers (Cucumaria frondosa and Holothuria leucospilota). Although tensilin converts the state of the dermis from soft to standard, it cannot convert from standard to stiff. In this study, we isolated and partially purified a novel stiffening factor from the dermis of Holothuria leucospilota. The factor stiffened the dermis in normal artificial sea water (ASW) but did not stiffen the soft dermis in Ca2+-free ASW. It also stiffened the dermis that had been converted to the standard state in Ca2+-free ASW by the action of tensilin. These results suggest that the factor produces the stiff dermis from the standard state but cannot work as a stiffener on the soft dermis. Its addition to longitudinal muscles of the sea cucumber produced no effects, suggesting that its effect is specific to the catch connective tissue. Its stiffening activity was susceptible to trypsin, meaning that it is a polypeptide, and its molecular mass estimated from gel filtration chromatography was 2.4 kDa.