bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel antibacterial dental glass‐ionomer cement
Authors: Weng Yiming , Guo Xia , Gregory Richard , Xie Dong 
Published: August 2010

Weng Y, Guo X, Gregory R, Xie D. A novel antibacterial dental glass‐ionomer cement.  Eur J Oral Sci 2010; 118: 531–534. © 2010 Eur J Oral Sci This study reports the synthesis and evaluation of a novel non‐leachable poly(quaternary ammonium salt) (PQAS)‐containing antibacterial glass‐ionomer cement. Fuji II LC cement was used for comparison. Compressive strength (CS) and Streptococcus mutans viability were used to evaluate strength and antibacterial activity, respectively. All specimens were conditioned in distilled water at 37°C before testing. After the addition of 1–30% PQAS, both cements showed a reduction in CS, of 25–95% for Fuji II LC and 13–78% for the experimental cement, and a reduction in S. mutans viability, of 40–79% for Fuji II LC and 40–91% for the experimental cement. The experimental cement showed less CS reduction and higher antibacterial activity compared with Fuji II LC. The result also indicates that the cements are permanently antibacterial, with no leaching of PQAS. It appears that the experimental cement is a clinically attractive dental restorative that can be potentially used for long‐lasting restorations as a result of its high mechanical strength and permanent antibacterial function.
Effects of Early Conceptus Signals on Circulating Immune Cells: Lessons from Domestic Ruminants
Authors: Ott Troy L. , Gifford Craig A. 
Published: August 2010

Citation Ott TL, Gifford CA. Effects of early conceptus signals on circulating immune cells: lessons from domestic ruminants. Am J Reprod Immunol 2010While there are few similarities between mechanisms for extending corpus luteum (CL) function during early pregnancy in ruminants and primates, there is increasing evidence that conceptus‐immune crosstalk in ruminants and primates affects the function of circulating immune cells at the very earliest stages of pregnancy. Most notable are changes in immune cell phenotypes with increased numbers of cells exhibiting the T regulatory phenotype and suppression of Th1 cytokines that promote tolerance to paternal alloantigens. Until recently, interferon τ produced by the ruminant trophectoderm was thought to act exclusively on the uterine endometrium; however, it is now clear that this unique embryonic interferon escapes the uterus and alters gene expression in the CL and in peripheral blood leukocytes (PBL). In fact, a large number of interferon‐stimulated genes are now known to be increased during early pregnancy in PBL. What is not known is how this conceptus‐immune system cross‐talk affects maternal immune status outside the reproductive tract. It is attractive to hypothesize that some of these effects are designed to counter‐balance progesterone‐induced immunosuppression so as not to place the dam at a greater risk of infection on top of the tremendous stresses already induced by pregnancy. Furthermore, recent evidence suggests that pregnancy induced changes in peripheral immune cells may aid in orchestrating establishment of pregnancy. Existing evidence points toward a greater convergence of systemic immune responses to early pregnancy signaling between ruminants and primates.
Today's treatments options for onychomycosis
Authors: Iorizzo Matilde , Piraccini Bianca Maria , Tosti Antonella 
Published: August 2010

SummaryOnychomycosis can be cured even if fungi are sometimes difficult to eradicate; the treatment is often lengthy and requires patience. The choice of agents should be based on numerous factors including patient's age and health, causative organism, clinical type of onychomycosis, number of affected nails and severity of nail involvement. We review current and future treatments for onychomycosis. We will also consider treatment options in patients with poor prognostic factors suggesting possible treatment failure.
Juvenile psoriasis and its clinical management: a European expert group consensus
Authors: Ståhle Mona , Atakan Nilgun , Boehncke Wolf‐Henning , Chimenti Sergio , Daudén Esteban , Giannetti Alberto , Hoeger Peter , Joly Pascal , Katsambas Andreas , Kragballe Knud , Lambert Jo , Ortonne Jean‐Paul , Prinz Joerg C. , Puig Lluís , Seyger Marieke , Strohal Robert , Van De Kerkhoff Peter , Sterry Wolfram 
Published: August 2010

Summary Background: Psoriasis, an inflammatory disorder of the skin, can significantly impact on a patient's quality of life, affecting their daily activities and families. The onset of psoriasis in childhood is quite common; however, the treatment of moderate‐to‐severe disease in this population is challenging, with a paucity of data reported and few licensed agents available. Methods: A Delphi survey was conducted among a panel of European expert dermatologists and physicians with a particular interest in pediatric inflammatory disorders. The survey covered the aspects of psoriasis types, psoriatic arthritis, diagnosis and treatment options in childhood. Results: A series of consensus opinions were reached, detailing the current practice in Europe for the diagnosis and treatment of psoriasis in childhood. These opinions are presented in the context of evidence from the literature and the current licensure status and indications of therapies for psoriasis in childhood. Conclusions: These data provide detailed information on the current practices in Europe for treating psoriasis in childhood.
Physical activity positively predicts bone architecture and bone strength in adolescent males and females
Authors: McKay Heather , Liu Danmei , Egeli Deetria , Boyd Steven , Burrows Melonie 
Published: August 2010

AbstractAims:  Physical activity (PA) has positive effects on bone accrual and geometry in children during growth. However, we do not know how PA influences adaptations in bone architecture during growth. We evaluated the contribution of PA to bone density, architecture and strength in adolescents.Methods:  We used HR‐pQCT (XtremeCT, Scanco Medical) to assess cross‐sectional moments of inertia [Imin, Imax (mm4)], total bone density (Tt.Dn, mg HA/cm3), total bone area (Tt.Ar, mm2), cortical bone density (Ct.Dn, mg HA/cm3), cortical thickness (Ct.Th, μm), trabecular bone density (Tb.Dn, mg HA/cm3), trabecular number (Tb.N, mm−1) and trabecular thickness (Tb.Th, μm) at the distal tibia in 146 male and 132 female participants (15–20 years). We evaluated the contribution of impact loading PA (ImpactPA) and non‐impact loading PA (NoimpactPA) on bone (p < 0.05).Results:  ImpactPA explained 10% of variance in Imin (p = 0.000), and 12% of variance in Imax (p = 0.000) in male participants. In male participants, ImpactPA explained 6% of variance in Tt.Ar (p = 0.003). In female participants, ImpactPA explained 4% of variance in Tt.Dn (p = 0.011), 5% of variance in Tb.Dn (p = 0.004) and 8% of variance in Tb.N (p = 0.001).Conclusion:  Our findings suggest that ImpactPA is significantly associated with bone architecture and bone strength in adolescent males and females.
Impact of mothers' negative affectivity, parental locus of control and child‐feeding practices on dietary patterns of 3‐year‐old children: The MoBa Cohort Study
Authors: Ystrom Eivind , Barker Mary , Vollrath Margarete E. 
Published: August 2010

AbstractThe aims of the current study were to (1) identify dietary patterns in 3‐year‐old children; (2) investigate the extent to which negative affectivity, external parental locus of control and control‐oriented child‐feeding practices (pressure to eat and restriction) relate to these dietary patterns; and (3) to examine to what extent external parental locus of control and control‐oriented child‐feeding practices serve as mediators for these effects. This study was part of the Norwegian Mother and Child Cohort Study, comprising 14 122 mothers completing assessments at 6 months, 18 months and 3 years post‐partum. Factor analysis of the children's diet identified two weakly correlated dietary patterns, labeled ‘unhealthy’ and ‘wholesome’. Mothers high in negative affectivity perceived they had little control over their child's behaviour, which in turn was associated with both pressuring their child to eat and restricting the child's food intake and a less wholesome and a more unhealthy diet in the child. Pressuring the child to eat was independently associated with a less wholesome and a more unhealthy diet. Restricting the child's diet was associated with a more wholesome and a less unhealthy diet. These findings held after controlling for maternal smoking, education, age, body mass index, marital status, homemaker status and child gender.
Evaluation of the flexural strength and elastic modulus of resins used for temporary restorations reinforced with particulate glass fibre
Authors: Orsi Iara Augusta , Soares Rodrigo Gonçalves , Villabona Camilo Andrés , Panzeri Heitor 
Published: August 2010

doi: 10.1111/j.1741‐2358.2010.00410.x

Evaluation of the flexural strength and elastic modulus of resins used for temporary restorations reinforced with particulate glass fibreObjective:  The flexural strength and the elastic modulus of acrylic resins, Dencor, Duralay and Trim Plus II, were evaluated with and without the addition of silanised glass fibre.Materials and methods:  To evaluate the flexural strength and elastic modulus, 60 test specimens were fabricated with the addition of 10% ground silanised glass fibres for the experimental group, and 60 without the incorporation of fibres, for the control group, with 20 test specimens being made of each commercial brand of resin (Dencor, Duralay and Trim Plus II) for the control group and experimental group. After the test specimens had been completed, the flexural strength and elastic modulus tests were performed in a universal testing device, using the three‐point bending test. For the specimens without fibres the One‐Way Analysis of Variance and the complementary Tukey test were used, and for those with fibres it was not normal, so that the non‐parametric Mann‐Whitney test was applied.Results:  For the flexural strength test, there was no statistical difference (p > 0.05) between each commercial brand of resin without fibres [Duralay 84.32(±8.54), Trim plus 85.39(±6.74), Dencor 96.70(±6.52)] and with fibres (Duralay 87.18, Trim plus 88.33, Dencor 98.10). However, for the elastic modulus, there was statistical difference (p > 0.01) between each commercial brand of resin without fibres [Duralay 2380.64 (±168.60), Trim plus 2740.37(±311.74), Dencor 2595.42(±261.22)] and with fibres (Duralay 3750.42, Trim plus 3188.80, Dencor 3400.75).Conclusion:  The result showed that the incorporation of fibre did not interfere in the flexural strength values, but it increased the values for the elastic modulus.
Validation of the Brazilian versions of two inventories for measuring oral health‐related quality of life of edentulous subjects
Authors: de Souza Raphael Freitas , Terada Andrea Sayuri Silveira Dias , Vecchia Maria Paula Della , Regis Romulo Rocha , Zanini Ana Paula , Compagnoni Marco Antonio 
Published: August 2010

doi: 10.1111/j.1741‐2358.2010.00417.x

Validation of the Brazilian versions of two inventories for measuring oral health‐related quality of life of edentulous subjectsObjectives:  To analyse the validity of the Brazilian versions of OHIP‐EDENT and GOHAI as assessment tools of edentulous subjects’ OHRQoL.Background:  Inventories for measuring oral health‐related quality of life (OHRQoL) are important in clinical studies regarding oral rehabilitation. However, there is a need for comprehensive validation after translation into different cultural settings.Materials and methods:  The sample comprised of 100 complete denture wearers (29 men, 71 women, mean age of 65.2 ± 9.9 years). The associations between each OHRQoL inventory and other variables served as measurements of construct validity. Data analysis comprised the Spearman correlation test as well as multiple regression using the OHRQoL inventories as dependent variables and the other scales as determinants.Results:  Both OHRQoL inventories showed good correlation with denture satisfaction, whereas lower correlation coefficients were found among the inventories and the HAD subscales. Denture satisfaction alone explained 48% and 39% of the variance found for the OHIP‐EDENT and GOHAI, respectively, as assessed by multiple regression. A smaller effect was found for OHIP‐EDENT.Conclusion:  Both OHIP‐EDENT and GOHAI showed good construct validity for measurement of OHRQoL of edentulous subjects.
The influence of implant number and attachment type on maximum bite force of mandibular overdentures: a retrospective study
Authors: Bilhan Hakan , Geckili Onur , Mumcu Emre , Cilingir Altug , Bozdag Ergun 
Published: August 2010

doi: 10.1111/j.1741‐2358.2010.00421.x

The influence of implant number and attachment type on maximum bite force of mandibular overdentures: a retrospective studyAim:  Assessment of the influence of such factors as attachment type, number of implants, gender and age of patients on maximum bite force (MBF).Material and methods:  Sixty‐two edentulous patients (32 females, 30 males; aged 64.03 ± 10.07 years, range 42–90 years) with mandibular implant overdentures with various attachment types were included in the study and their MBFs were recorded. The results were evaluated statistically at a significance level of p < 0.05.Results:  The MBF in male patients was found to be statistically significantly (p < 0.05) higher than in female patients. No statistically significant differences (p > 0.05) were found with respect to age groups, attachment types and number of implants supporting the mandibular overdentures.Conclusion:  Within the limitations of this study, it is concluded that independent of the number applied, dental implants increase MBF in edentulous patients. While males show higher bite force, patient age and attachment type seem not to play an important role.
Spirituality, religion, social support and health among older Australian adults
Authors: Moxey Annette , McEvoy Mark , Bowe Steven , Attia John 
Published: August 2010

Aim:  To examine the impact of perceived importance of spirituality or religion (ISR) and religious service attendance (RSA) on health and well‐being in older Australians.Methods:  A cross‐sectional survey of 752 community‐dwelling men and women aged 55–85 years from the Hunter Region, New South Wales.Results:  Overall, 51% of participants felt spirituality or religion was important in their lives and 24% attended religious services at least 2–3 times a month. In univariate regression analyses, ISR and RSA were associated with increased levels of social support (P < 0.001). However, ISR was also associated with more comorbidities (incidence–rate ratio= 1.2, 95% confidence interval 1.08–1.33). There were no statistically significant associations between ISR or RSA and other measures such as mental and physical health.Conclusion:  Spirituality and religious involvement have a beneficial impact on older Australians' perceptions of social support, and may enable individuals to better cope with the presence of multiple comorbidities later in life.
A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor‐κB pathway in skeletal muscle
Authors: Laure Lydie , Danièle Nathalie , Suel Laurence , Marchand Sylvie , Aubert Sophie , Bourg Nathalie , Roudaut Carinne , Duguez Stéphanie , Bartoli Marc , Richard Isabelle 
Published: August 2010

A multiprotein complex encompassing a transcription regulator, cardiac ankyrin repeat protein (CARP), and the calpain 3 protease was identified in the N2A elastic region of the giant sarcomeric protein titin. The present study aimed to investigate the function(s) of this complex in the skeletal muscle. We demonstrate that CARP subcellular localization is controlled by the activity of calpain 3: the higher the calpain 3, the more important the sarcomeric retention of CARP. This regulation would occur through cleavage of the N‐terminal end of CARP by the protease. We show that, upon CARP over‐expression, the transcription factor nuclear factor NF‐κB p65 DNA‐binding activity decreases. Taken as a whole, CARP and its regulator calpain 3 appear to occupy a central position in the important cell fate‐governing NF‐κB pathway. Interestingly, the expression of the atrophying protein MURF1, one of NF‐κB main targets, remains unchanged in presence of CARP, suggesting that the pathway encompassing calpain3/CARP/NF‐κB does not play a role in muscle atrophy. With NF‐κB also having anti‐apoptotic effects, the inability of calpain 3 to lower CARP‐driven inhibition of NF‐κB could reduce muscle cell survival, hence partly accounting for the dystrophic pattern observed in limb girdle muscular dystrophy 2A, a pathology resulting from the protease deficiency.Structured digital abstract
MINT‐7990388: Titin (uniprotkb:Q8WZ42) physically interacts (MI:0915) with CARP (uniprotkb:Q9CR42) by two hybrid (MI:0018)
MINT‐7990374: calpain 3 (uniprotkb:P20807) physically interacts (MI:0915) with Titin (uniprotkb:Q8WZ42) by two hybrid (MI:0018)
MINT‐7990342: calpain 3 (uniprotkb:P20807) physically interacts (MI:0915) with CARP (uniprotkb:Q9CR42) by two hybrid (MI:0018)
NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon Methanosarcina mazei Gö1
Authors: Weidenbach Katrin , Ehlers Claudia , Kock Jutta , Schmitz Ruth A. 
Published: August 2010

We report here on the formation of a complex between the two NrpR homologs present in Methanosarcina mazei Gö1 and their binding properties to the nifH and glnK1 promoters. Reciprocal co‐chromatography demonstrated that NrpRI forms stable complexes with NrpRII (at an NrpRI : NrpRII molar ratio of ∼ 1 : 3), which are not affected by 2‐oxoglutarate. Promoter‐binding, analyses using DNA‐affinity chromatography and electrophoretic gel mobility shift assays, verified that NrpRII is not able to bind to either the nifH promoter or the glnK1 promoter except when in complex with NrpRI. Specific binding of NrpRI to the nifH and glnK1 promoters was shown to be highly sensitive to 2‐oxoglutarate, regardless of whether only NrpRI, or NrpRI in complex with NrpRII, bound to the promoter. Finally, strong interactions between NrpRII and the general transcription factors TATA‐binding proteins (TBP) 1–3 and the general transcription factor TFIIB (TFB) were demonstrated, interactions which are also sensitive to 2‐oxoglutarate. On the basis of these findings we propose the following: under nitrogen sufficiency NrpRII binds from solution to either the nifH promoter or the glnK1 promoter by simultaneously contacting NrpRI and TBP plus TFB, resulting in full repression of transcription; whereas, under nitrogen limitation, increasing 2‐oxoglutarate concentrations significantly decrease the binding of NrpRI to the operator as well as the binding of NrpRII to TBP and TFB, ultimately allowing recruitment of RNA polymerase to the promoter.Structured digital abstract
MINT‐7990058: NrpRII (uniprotkb:Q8PVJ4) physically interacts (MI:0915) with TBP3 (uniprotkb:Q8PUZ4) by pull down (MI:0096)
MINT‐7989998: NrpRII (uniprotkb:Q8PVJ4) physically interacts (MI:0915) with TBP1 (uniprotkb:Q8PY37) by pull down (MI:0096)
MINT‐7989971, MINT‐7989984: NrpRII (uniprotkb:Q8PVJ4) physically interacts (MI:0915) with NrpRI (uniprotkb:Q8PXY1) by pull down (MI:0096)
MINT‐7990028: NrpRII (uniprotkb:Q8PVJ4) physically interacts (MI:0915) with TBP2 (uniprotkb:Q8PY36) by pull down (MI:0096)
MINT‐7990087: NrpRII (uniprotkb:Q8PVJ4) physically interacts (MI:0915) with TFP (uniprotkb:Q977U3) by pull down (MI:0096)
MINT‐7990149: NrpRII (uniprotkb:Q8PVJ4) and NrpRI (uniprotkb:Q8PXY1) bind (MI:0407) by molecular sieving (MI:0071)
Autolytic activity of human calpain 7 is enhanced by ESCRT‐III‐related protein IST1 through MIT–MIM interaction
Authors: Osako Yohei , Maemoto Yuki , Tanaka Ryohei , Suzuki Hironori , Shibata Hideki , Maki Masatoshi 
Published: August 2010

Calpain 7, a mammalian ortholog of yeast Cpl1/Rim13 and fungal PalB, is an atypical calpain that lacks a penta‐EF‐hand domain. Previously, we reported that a region containing a tandem repeat of microtubule‐interacting and transport (MIT) domains in calpain 7 interacts with a subset of endosomal sorting complex required for transport (ESCRT)‐III‐related proteins, suggesting involvement of calpain 7 in the ESCRT system. Although yeast and fungal calpains are thought to be involved in alkaline adaptation via limited proteolysis of specific transcription factors, proteolytic activity of calpain 7 has not been demonstrated yet. In this study, we investigated the interaction between calpain 7 and a newly reported ESCRT‐III family member, increased sodium tolerance‐1 (IST1), which possesses two different types of MIT‐interacting motifs (MIM1 and MIM2). We found that glutathione‐S‐transferase (GST)‐fused tandem MIT domains of calpain 7 (calpain 7MIT) pulled down FLAG‐tagged IST1 expressed in HEK293T cells. Coimmunoprecipitation assays with various deletion or point mutants of epitope‐tagged calpain 7 and IST1 revealed that both repetitive MIT domains and MIMs are required for efficient interaction. Direct MIT–MIM binding was confirmed by a pulldown experiment with GST‐fused IST1 MIM and purified recombinant calpain 7MIT. Furthermore, we found that the GST–MIM protein enhances the autolysis of purified Strep‐tagged monomeric green fluorescent protein (mGFP)‐fused calpain 7 (mGFP–calpain 7–Strep). The autolysis was almost completely abolished by 10 mmN‐ethylmaleimide but only partially inhibited by 1 mm leupeptin or E‐64. The putative catalytic Cys290‐substituted mutant (mGFP–calpain 7C290S–Strep) showed no autolytic activity. These results demonstrate for the first time that human calpain 7 is proteolytically active, and imply that calpain 7 is activated in the ESCRT system.Structured digital abstract
MINT‐7990193, MINT‐7990213, MINT‐7990233: calpain 7 (uniprotkb:Q9Y6W3) physically interacts (MI:0915) with IST1 (uniprotkb:P53990) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7990176: calpain 7 (uniprotkb:Q9Y6W3) physically interacts (MI:0915) with IST1 (uniprotkb:P53990) by pull down (MI:0096)
MINT‐7990252: IST1 (uniprotkb:P53990) binds (MI:0407) to calpain 7 (uniprotkb:Q9Y6W3) by pull down (MI:0096)
Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft
Authors: Renko Miha , Požgan Urška , Majera Dušana , Turk Dušan 
Published: August 2010

Cathepsin B (EC 3.4.22.1) is one of the most versatile human cysteine cathepsins. It is important for intracellular protein degradation under normal conditions and is involved in a number of pathological processes. The occluding loop makes cathepsin B unique among cysteine cathepsins. This ∼ 20 residue long insertion imbedded into the papain‐like protease scaffold restricts access to the active site cleft and endows cathepsin B with its carboxydipeptidase activity. Nevertheless, the enzyme also exhibits endopeptidase activity and is inhibited by stefins and cystatins. To clarify the structural properties of the occluding loop upon the binding of stefins, we determined the crystal structure of the complex between wild‐type human stefin A and wild‐type human cathepsin B at 2.6 Å resolution. The papain‐like part of cathepsin B structure remains unmodified, whereas the occluding loop residues are displaced. The part enclosed by the disulfide bridge containing histidines 110 and 111 (i.e. the ‘lasso’ part) is rotated by ∼ 45° away from its original position. A comparison of the structure of the unliganded cathepsin B with the structure of the proenzyme, its complexes with chagasin and stefin A shows that the magnitude of the shift of the occluding loop is related to the size of the binding region. It is smallest in the procathepsin structures and increases in the series of complexes with stefin A and chagasin, although it has no impact on the binding constant. Hence, cathepsin B can dock inhibitors and certain substrates regardless of the size of the binding region.Structured digital abstract
MINT‐7990451: Stefin‐A (uniprotkb:P01040) and Cathepsin B (uniprotkb:P07858) bind (MI:0407) by x‐ray crystallography (MI:0114)