bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulated microRNAs in the differential diagnosis of malignant pleural mesothelioma
Authors: Gee Gretchen V. , Koestler Devin C. , Christensen Brock C. , Sugarbaker David J. , Ugolini Donatella , Ivaldi Giovanni P. , Resnick Murray B. , Houseman E. Andres , Kelsey Karl T. , Marsit Carmen J. 
Published: March 2010

AbstractMalignant pleural mesothelioma (MPM) is a rapidly fatal disease whose diagnosis, particularly through less invasive techniques such as analysis of pleural effusion, can be challenging. Currently, a commercially available diagnostic test based on microRNA (miRNA) expression patterns is purported to distinguish between mesothelioma and lung adenocarcinoma. Yet, the biological basis of this technology has not been reported in the literature, and little research has been aimed at determining how differential miRNA expression contributes to the differences in pathogenesis between these diseases, both of which can be caused by asbestos exposure. We sought to illuminate the molecular differences between mesothelioma and lung adenocarcinoma by using miRNA microarrays to identify patterns in the most differentially expressed miRNAs. From this, we identified a panel of miRNAs, including members of the miR‐200 gene family, that were all downregulated in MPM compared to lung adenocarcinoma. Using the more sensitive detection method of quantitative RT‐PCR on an independent series of tumors, we validated the specificity of these alterations in 100 MPMs and 32 lung adenocarcinomas. Statistical analysis reveals that these miRNAs exceed the current recommendations for biomarkers and could greatly aid in the differential diagnosis. Further examination led us to predict that they act as redundant regulators of wnt signaling and suggests a role for this pathway in tumor progression. This research points to novel approaches using miRNAs whose decreased expression is unique to mesothelioma as potentially suitable for rapid diagnosis and reveals prospective new targets for the treatment of this deadly disease.
Early detection of lung adenocarcinoma in sputum by a panel of microRNA markers
Authors: Yu Lei , Todd Nevins W. , Xing Lingxiao , Xie Ying , Zhang Howard , Liu Zhenqiu , Fang HongBin , Zhang Jian , Katz Ruth L. , Jiang Feng 
Published: March 2010

AbstractAdenocarcinoma is the most common type of lung cancer, the leading cause of cancer deaths in the world. Early detection is the key to improve the survival of lung adenocarcinoma patients. We have previously shown that microRNAs (miRNAs) were stably present in sputum and could be applied to diagnosis of lung cancer. The aim of our study was to develop a panel of miRNAs that can be used as highly sensitive and specific sputum markers for early detection of lung adenocarcinoma. Our study contained 3 phases: (i) marker discovery using miRNA profiling on paired normal and tumor lung tissues from 20 patients with lung adenocarcinoma; (ii) marker optimization by real‐time reverse transcription‐quantitative polymerase chain reaction on sputum of a case–control cohort consisting of 36 cancer patients and 36 health individuals and (iii) validation on an independent set of 64 lung cancer patients and 58 cancer‐free subjects. From the surgical tissues, 7 miRNAs with significantly altered expression were identified, of which “4” were overexpressed and “3” were underexpressed in all 20 tumors. On the sputum samples of the case–control cohort, 4 (miR‐21, miR‐486, miR‐375 and miR‐200b) of the 7 miRNAs were selected, which in combination produced the best prediction in distinguishing lung adenocarcinoma patients from normal subjects with 80.6% sensitivity and 91.7% specificity. Validation of the marker panel in the independent populations confirmed the sensitivity and specificity that provided a significant improvement over any single one alone. The sputum markers demonstrated the potential of translation to laboratory settings for improving the early detection of lung adenocarcinoma.
Bioactivity of immobilized EGF on self‐assembled monolayers: Optimization of the immobilization process
Authors: Gonçalves Raquel , Martins Maria Cristina Lopes , Oliveira Maria José , Almeida‐Porada Graça , Barbosa Mário Adolfo 
Published: March 2010

AbstractLast trends in Biomaterials focus the mimic of cellular environments capable to control cellular responses. Epidermal growth factor (EGF) is a pleiotropic cytokine known to regulate cell proliferation, differentiation, and death. This study aims to optimize the immobilization of EGF on 11‐mercapto‐1‐undecyl‐tetra(ethylene)glycol (EG4)—self‐assembled monolayers (SAMs) and to establish a new model surface to study EGF‐mediated signaling. Gold substrates were modified with a monolayer of EG4 and N,N′‐carbonyldiimidazole (CDI) was used to activate hydroxyl terminated groups of EG4‐SAMs. EGF was then immobilized on activated EG4‐SAMs at pH 7.4, 4°C, and 100 rpm. Different immobilization reaction times were tested as well as different CDI concentrations to optimize the reaction conditions and obtain a range of immobilized EGF concentrations on the surfaces. Surface characterization of EGF‐SAMs was performed using radiolabeling, water contact angle measurements, X‐ray photoelectron spectroscopy, and ELISA. Phosphorylation of EGFR on BT‐20 breast cancer cell line by EGF‐SAMs was tested by immunostaining. EGF was successfully immobilized on EG4‐SAMs, at 4°C and pH 7.4 in a range of concentrations from 3.6 ± 0.8 to 17.6 ± 1.5 ng/cm2. The concentration of EGF increases with immobilization time and with the CDI concentration reaching the maximum for surfaces activated with 30 mg/mL of CDI after 48 h. The bioactivity of EGF‐SAMs was confirmed by immunostaining of phospho‐EGFR of BT‐20 cells. This study described EGF immobilization on EG4‐SAMs at different concentrations, which could be important surface models to study specific protein interactions at the molecular level evolving EGF‐family of proteins. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2010
Differences in the bone differentiation properties of MC3T3‐E1 cells on polished bulk and sputter‐deposited titanium specimens
Authors: Oya Kei , Tanaka Yuta , Moriyama Yoshihisa , Yoshioka Yuki , Kimura Tsuyoshi , Tsutsumi Yusuke , Doi Hisashi , Nomura Naoyuki , Noda Kazuhiko , Kishida Akio , Hanawa Takao 
Published: March 2010

AbstractThe roughness and cleanness of a titanium surface must be controlled in order to investigate the expression mechanism of hard tissue compatibility on titanium. In this study, osteogenic MC3T3‐E1 cells were cultured and differentiation‐induced on bulk and sputter‐deposited titanium specimens, and the osteogenesis were investigated. For the preparation of bulk specimens, titanium discs were mirror‐polished. On the other hand, titanium was sputter‐deposited on smooth and clean cover glasses as sputter‐deposited specimens. As a result, no significant difference was observed in the cell morphology and attached number. On the other hand, the time showing maximum activity in the alkaline phosphatase and gene expressions, which are related to bone differentiation on the bulk titanium, were superior to those on the sputter‐deposited titanium. From the surface observation of the specimens with a scanning electron microscope and a scanning probe microscope, the surface on the sputter‐deposited titanium was more uniform and cleaner than that on the bulk titanium. According to X‐ray photoelectron spectroscopy, the thickness of surface oxide film on the sputter‐deposited titanium was smaller than that on the bulk titanium. In addition, the proportions of TiO and Ti2O3 in the surface oxide film on the sputter‐deposited titanium were larger than those on the bulk titanium. These differences might influence the differentiation of osteoblastic cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Identification of osteoconductive and biodegradable polymers from a combinatorial polymer library
Authors: Brey Darren M. , Chung Cindy , Hankenson Kurt D. , Garino Jonathon P. , Burdick Jason A. 
Published: March 2010

AbstractCombinatorial polymer syntheses are now being utilized to create libraries of materials with potential utility for a wide variety of biomedical applications. We recently developed a library of photopolymerizable and biodegradable poly(β‐amino ester)s (PBAEs) that possess a range of tunable properties. In this study, the PBAE library was assessed for candidate materials that met design criteria (e.g., physical properties such as degradation and mechanical strength and in vitro cell viability and osteoconductive behavior) for scaffolding in mineralized tissue repair. The most promising candidate, A6, was then processed into three‐dimensional porous scaffolds and implanted subcutaneously and only presented a mild inflammatory response. The scaffolds were then implanted intramuscularly and into a critical‐sized cranial defect either alone or loaded with bone morphogenetic protein‐2 (BMP‐2). The samples in both locations displayed mineralized tissue formation in the presence of BMP‐2, as evident through radiographs, micro‐computed tomography, and histology, whereas samples without BMP‐2 showed minimal or no mineralized tissue. These results illustrate a process to identify a candidate scaffolding material from a combinatorial polymer library, and specifically for the identification of an osteoconductive scaffold with osteoinductive properties via the inclusion of a growth factor. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Furan ring recyclization in 2‐furfurylthieno[2,3‐b]pyridines: An intramolecular N‐alkylation of pyrrole ring under acid conditions
Authors: Kosulina Darya Yu. , Vasilin Vladimir K. , Stroganova Tatyana A. , Kaklyugina Tatyana Ya. , Krapivin Gennady D. 
Published: March 2010

Abstract
magnified image The preparation of new 3‐amino‐2‐furfurylthieno[2,3‐b]pyridines (1a, 1b, 69–80%) is described. Subsequent acidic rearrangement of 1a, 1b afforded two new annulated heterocyclic products, 5a, 5b, pyrrolothieno[2,3‐b]pyridines (45–74%), and 6, pyridothieno[2,3‐b]pyrrolizine (22%), depending on reaction conditions. J. Heterocyclic Chem., (2010).
An efficient one‐pot three component synthesis of 1,2‐dihydro‐1‐arylnaphtho[1,2‐e][1,3]oxazine‐3‐ones using montmorillonite K10 under solvent free conditions
Authors: Kantevari Srinivas , Vuppalapati Srinivasu V. N. , Bantu Rajashaker , Nagarapu Lingaiah 
Published: March 2010

Abstract
magnified image An efficient green protocol for the preparation of 1,2‐dihydro‐1‐arylnaphtho[1,2‐e][1,3]oxazine‐3‐ones employing a three‐component one‐pot condensation reaction of β‐naphthol, aromatic aldehyde, urea or thiourea in the presence of montmorillonite K10 clay under solvent free conditions has been described. The present procedure offers advantages such as shorter reaction time (<90 min), simple workup, excellent yields, recovery and reusability of catalyst. J. Heterocyclic Chem., (2010).
Rearrangement of 2‐bromo‐N‐quinoline‐8‐yl‐acetamide leading to new heterocycle
Authors: Kalita Dipjyoti , Baruah Jubaraj B. 
Published: March 2010

Abstract
magnified image A four fused rings containing heterocyclic compound is formed in the reaction between 2‐bromo‐N‐quinoline‐8‐yl‐acetamide, 2‐(4‐methoxyphenyl)ethylamine, and acetone in the presence of potassium carbonate; the heterocycle undergoes further reaction with perchloric acid to form a perchlorate salt of a quinoxaline derivative. J. Heterocyclic Chem., (2010).
A novel synthetic route to synthesize 2,4,8,10‐tetraoxaspiro[5.5]‐undecane from formaldehyde under hydrothermal conditions
Authors: Maheen Gull , Tian Ge , Song Zhiguang , He Chao , Shi Zhan , Liu Ziwei , Yuan Hongming , Feng Shouhua 
Published: March 2010

Abstract
magnified image We report here the hydrothermal synthesis of 2,4,8,10‐tetraoxaspiro[5.5]‐undecane by using formaldehyde, the only precursor, in the presence of kaolin clay and boric acid. The reported method is the simplest one to synthesize the aforementioned product. J. Heterocyclic Chem., (2010).
Simple single‐step single‐enzyme synthesis of [14C]‐GSH
Authors: de Keczer Steve A. , Voronin Tatyana , Yao Jennifer , Zhang Fang‐Jie , Masjedizadeh Mohammad R. 
Published: March 2010

AbstractThe tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
An investigation into the impact of vacation travel on the tourist
Authors: Alexander Zoë , Bakir Ali , Wickens Eugenia 
Published: March 2010

AbstractThis paper explored the impact of vacation travel on the ‘self’ using a questionnaire survey and interviews. Vacation travel emerged as a form of learning about the self, a journey of self‐discovery rather than self‐recovery. Travel impacts are predominantly self‐centered, producing changes that provide opportunities for personal growth. Experiences leading to these changes are mainly influenced by interactions with the ‘other’. Importantly, no significant association was found between personality type and being impacted, and no difference was found between a pleasurable and a miserable experience and being impacted. Both findings offer a contribution to knowledge and a platform for further research. Copyright © 2010 John Wiley & Sons, Ltd.
Two singular point linear Hamiltonian systems with an interface condition
Authors: Behncke Horst , Hinton Don 
Published: February 2010

AbstractWe consider the problem of a linear Hamiltionian system on ℝ with an interface condition which we take to be at x = 0. Assuming limit point conditions at ±∞, we prove the problem is uniquely solvable, and a resolvent is constructed. Our method of solution is to map the problem onto a half line problem of double size and apply the theory of half line problems. A Titchmarsh‐Weyl function is associated with the problem, and a unitary transform is constructed which maps the differential operator onto the multiplication operator in the Hilbert space determined by the spectral function ρ (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Restless legs syndrome in a population of northern Tanzania: A community‐based study
Authors: Winkler Andrea Sylvia , Trendafilova Anna , Meindl Michael , Kaaya John , Schmutzhard Erich , Kassubek Jan 
Published: March 2010

AbstractRestless legs syndrome (RLS) is a highly prevalent movement disorder. However, prevalences seem to vary amongst different ethnicities. To date, no community‐based prevalence studies on RLS have been reported from the African continent. We have conducted a community‐based, door‐to‐door study in northern Tanzania. Over a period of 16 months, 7,654 people aged 14 years and older were screened for the RLS Essential Diagnostic Criteria. Sampling was performed according to the method of “multistage cluster sampling.” People who screened positive where reinterviewed and physically examined by a specialist neurologist. During the screening phase, 10 people answered “yes” to at least one of the screening questions. After reinterviewing those people, the result was confirmed in five people only. After careful re‐evaluation of the results, only one person was diagnosed with RLS. Because of methodological limitations a definite prevalence may only be calculated from larger population‐based studies of different African ethnicities with screening questions adapted to the cultural context. © 2010 Movement Disorder Society
Complex paroxysmal nocturnal behaviors in Parkinson's disease
Authors: Manni Raffaele , Terzaghi Michele , Repetto Alessandra , Zangaglia Roberta , Pacchetti Claudio 
Published: March 2010

AbstractComplex paroxysmal nocturnal motor behavioral disorders (CPNBs) are frequently reported in patients with Parkinson's disease (PD). REM sleep behavior disorder (RBD) is reported in at least a third of PD patients, although CPNB episodes can also occur on arousal from NREM sleep. It is important to establish the nature of CPNBs occurring in PD, as the different types have different neurobiological significance and clinical importance, and also different treatments. Ninety‐six PD patients with and without CPNBs were submitted to overnight in‐hospital video‐polysomnography. Of these, 76 (47 men) were included in the study analysis: these were patients in whom it was possible to establish the presence or absence of CPNBs and to obtain a clear‐cut diagnosis of the nature of the CPNBs reported. The CPNBs were found to be RBD episodes in 39 cases (87%) and nonRBD episodes in 6 (13%) (arousal‐related episodes arising from NREM sleep in 3 cases and from REM sleep in 2 cases, parasomnia overlapping syndrome in 1 case). In 4 of the 6 subjects with nonRBD episodes, these occurred upon arousal at the end of an obstructive apnoeic event. Our data confirm that CPNBs in PD are, in most cases, RBD episodes. However, arousal‐related episodes accounted for 13% of the CPNBs observed in our sample and occurred in close temporal association with sleep‐disordered breathing (SDB). The arousal system is defective in extrapyramidal diseases due to neurodegenerative changes involving the brain stem reticular network; against this background, a trigger effect of SDB on CPNBs, through induction of abrupt arousal, may be hypothesized. © 2010 Movement Disorder Society
Long‐term antidyskinetic efficacy of amantadine in Parkinson's disease
Authors: Wolf Elisabeth , Seppi Klaus , Katzenschlager Regina , Hochschorner Guenter , Ransmayr Gerhard , Schwingenschuh Petra , Ott Erwin , Kloiber Iris , Haubenberger Dietrich , Auff Eduard , Poewe Werner 
Published: March 2010

AbstractSeveral randomized placebo‐controlled trials have consistently shown antidyskinetic effects of amantadine in levodopa treated patients with advanced Parkinson's disease (PD). However, all of these were of short duration and there have been claims that the effect of amantadine on levodopa induced dyskinesias (LID's) wear off after about 9 months of treatment. This randomized placebo‐controlled parallel‐group study was performed to assess the long‐term antidyskinetic effect of amantadine in 32 PD patients, who after having been on stable amantadine therapy for LID over at least one year‐ were switched in a double blind manner to amantadine or placebo and followed for 3 weeks. Dyskinesia duration and intensity were assessed by UPDRS IV items 32 and 33 as well as by patient's diaries. The primary outcome was the score change of UPDRS IV items 32 + 33 between baseline and 3 weeks after treatment as well as the between treatment group comparison of the score change of UPDRS IV items 32 + 33. There was a significant increase of UPDRS IV items 32 + 33 in patients treated with placebo from 3.06 (95% CI, 2.1–4.03) at baseline to 4.28 (95% CI, 3.1–5.4) at three‐week follow‐up (P = 0.02) compared with no significant change between baseline 3.2 (95% CI, 2.1–4.4) to follow‐up 3.6 (95% CI, 2.3–4.8) in patients staying on amantadine. These findings argue for long‐term antidyskinetic efficacy of amantadine in PD patients with LID's. © 2010 Movement Disorder Society
Cell‐free expression profiling of E. coli inner membrane proteins
Authors: Schwarz Daniel , Daley Daniel , Beckhaus Tobias , Dötsch Volker , Bernhard Frank 
Published: March 2010

AbstractThe high versatility and open nature of cell‐free expression systems offers unique options to modify expression environments. In particular for membrane proteins, the choice of co‐translational versus post‐translational solubilization approaches could significantly modulate expression efficiencies and even sample qualities. The production of a selection of 134 α‐helical integral membrane proteins of the Escherichia coli inner membrane proteome focussing on larger transporters has therefore been evaluated by a set of individual cell‐free expression reactions. The production profiles of the targets in different cell‐free expression modes were analyzed independently by three screening strategies. Translational green fluorescent protein fusions were analyzed as monitor for the formation of proteomicelles after cell‐free expression of membrane proteins in the presence of detergents. In addition, two different reaction configurations were implemented and performed either by robotic semi‐throughput approaches or by individually designed strategies. The expression profiles were specified for the particular cell‐free modes and overall, the production of 87% of the target list could be verified and approximately 50% could already be synthesized in preparative scales. The expression of several selected targets was up‐scaled to milliliter volumes and milligram amounts of production. As an example, the flavocytochrome YedZ was purified and its sample quality was demonstrated.
Conformational analysis of 3,3‐dimethyl‐3‐silathiane, 2,3,3‐trimethyl‐3‐silathiane and 2‐trimethylsilyl‐3,3‐dimethyl‐3‐silathiane—preferred conformers, barriers to ring inversion and substituent effects
Authors: Kirpichenko Svetlana V. , Kleinpeter Erich , Shainyan Bagrat A. 
Published: March 2010

AbstractThe first conformational analysis of 3‐silathiane and its C‐substituted derivatives, namely, 3,3‐dimethyl‐3‐silathiane 1, 2,3,3‐trimethyl‐3‐silathiane 2, and 2‐trimethylsilyl‐3,3‐dimethyl‐3‐silathiane 3 was performed by using dynamic NMR spectroscopy and B3LYP/6‐311G(d,p) quantum chemical calculations. From coalescence temperatures, ring inversion barriers ΔG≠ for 1 and 2 were estimated to be 6.3 and 6.8 kcal/mol, respectively. These values are considerably lower than that of thiacyclohexane (9.4 kcal/mol) but slightly higher than the one of 1,1‐dimethylsilacyclohexane (5.5 kcal/mol). The conformational free energy for the methyl group in 2 (−ΔG° = 0.35 kcal/mol) derived from low‐temperature 13C NMR data is fairly consistent with the calculated value. For compound 2, theoretical calculations give ΔE value close to zero for the equilibrium between the 2‐Meax and 2‐Meeq conformers. The calculated equatorial preference of the trimethylsilyl group in 3 is much more pronounced (−ΔG° = 1.8 kcal/mol) and the predominance of the 3‐SiMe3 eq conformer at room temperature was confirmed by the simulated 1H NMR and 2D NOESY spectra. The effect of the 2‐substituent on the structural parameters of 2 and 3 is discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Superacid mediated hydroxyalkylation reaction of 1,2,3‐indanetrione: a theoretical study
Authors: Nieto Daniel Romero , Zolotukhin Mikhail G. , Fomina Lioudmila , Fomine Serguei 
Published: March 2010

AbstractEnergies of mono‐ and multiprotonation for 1,2,3‐indanetrione and ninhydrin in triflic acid (TFSA) media were estimated at PBE0/aug‐cc‐pvtz//6‐31+G** level of theory. The reactivity of formed intermediates in the reaction of aromatic electrophilic substitution has been studied at the same level of theory. It appears that the basicity of carbonyl groups in 1,2,3‐indanetrione is extremely low due to mutual influences of carbonyl groups. Carbonyl 2 is the least basic but the most reactive in accordance with experiment. Calculations demonstrated that monoprotonated intermediates are the principal reactive species in the reaction of hydroxyalkylation of 1,2,3‐indanetrione in TFSA. A new isomerization mechanism of 2,2‐diaryl‐1,3‐indanediones to 3‐(diarylmethylene)isobenzofuranones in TFSA media has been proposed. Copyright © 2010 John Wiley & Sons, Ltd.