bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Selenaion koniopes n. gen., n. sp., an Amoeba that Represents a New Major Lineage within Heterolobosea, Isolated from the Wieliczka Salt Mine
Authors: Park Jong Soo , De Jonckheere Johan F. , Simpson Alastair G. B. 
Published: August 2012

AbstractA new heterolobosean amoeba, Selenaion koniopes n. gen., n. sp., was isolated from 73‰ saline water in the Wieliczka salt mine, Poland. The amoeba had eruptive pseudopodia, a prominent uroid, and a nucleus without central nucleolus. Cysts had multiple crater‐like pore plugs. No flagellates were observed. Transmission electron microscopy revealed several typical heterolobosean features: flattened mitochondrial cristae, mitochondria associated with endoplasmic reticulum, and an absence of obvious Golgi dictyosomes. Two types of larger and smaller granules were sometimes abundant in the cytoplasm—these may be involved in cyst formation. Mature cysts had a fibrous endocyst that could be thick, plus an ectocyst that was covered with small granules. Pore plugs had a flattened dome shape, were bipartite, and penetrated only the endocyst. Phylogenies based on the 18S rRNA gene and the presence of 18S rRNA helix 17_1 strongly confirmed assignment to Heterolobosea. The organism was not closely related to any described genus, and instead formed the deepest branch within the Heterolobosea clade after Pharyngomonas, with support for this deep‐branching position being moderate (i.e. maximum likelihood bootstrap support—67%; posterior probability—0.98). Cells grew at 15–150‰ salinity. Thus, S. koniopes is a halotolerant, probably moderately halophilic heterolobosean, with a potentially pivotal evolutionary position within this large eukaryote group.
Coordinate Synthesis but Discrete Localization of Homologous N‐Glycosylated Proteins, CLP and CLB, in Naegleria pringsheimi Flagellates
Authors: Baek In keol , Chung Sunglan , Suh Mi Ra , Hwang Deog Su , Kang Dongmin , Lee Joohun 
Published: August 2012

AbstractThe synchronous amoebae‐to‐flagellates differentiation of Naegleria pringsheimi has been used as a model system to study the formation of eukaryotic flagella. We cloned two novel genes, Clp, Class I on plasma membrane and Clb, Class I at basal bodies, which are transiently expressed during differentiation and characterized their respective protein products. CLP (2,087 amino acids) and CLB (1,952 amino acids) have 82.9% identity in their amino acid sequences and are heavily N‐glycosylated, leading to an ~ 100 × 103 increase in the relative molecular mass of the native proteins. In spite of these similarities, CLP and CLB were localized to distinct regions: CLP was present on the outer surface of the plasma membrane, whereas CLB was concentrated at a site where the basal bodies are assembled and remained associated with the basal bodies. Oryzalin, a microtubule toxin, inhibited the appearance of CLP on the plasma membrane, but had no effect on the concentration of CLB at its target site. These data suggest that N. pringsheimi uses separate mechanisms to transport CLP and CLB to the plasma membrane and to the site of basal body assembly, respectively.
Resorbable Tricalcium Phosphates for Bone Tissue Engineering: Influence of SrO Doping
Authors: DeVoe Ken , Banerjee Shashwat , Roy Mangal , Bandyopadhyay Amit , Bose Susmita 
Published: August 2012

Tricalcium Phosphate (TCP) was doped with 0.5, 2.0, and 5.0 mol% Strontium Oxide (SrO) through the wet precipitation process to investigate the influence of SrO on the biological and mechanical properties of TCP. XRD results showed that SrO doping did not change the phase stability of the β‐phase of TCP during sintering at 1120°C. The apparent density of TCP increased from 95.1 ± 2.5% to 99.4 ± 0.5% with the addition of 5.0 mol% SrO doping. SrO doping also increased the compressive strength of pure β‐TCP from 121.1 ± 21.4 MPa to 185.9 ± 16.7 MPa with 5 mol% SrO addition. Initially, the addition of 0.5 and 2.0 mol% SrO had a statistically insignificant impact on the as‐processed samples' compressive strength. When the samples were immersed in a simulated body fluid for 12 weeks, the addition of SrO improved the compressive strength from 109.4 ± 22.9 MPa for pure TCP to 208.8 ± 34.6 MPa with 5.0 mol% SrO addition. An improvement in hFOB cell attachment and proliferation was observed for all dopant concentrations using field emission scanning electron microscope (FE‐SEM) imaging and MTT assay studies, indicating excellent biocompatibility of all the samples. 2.0 mol% SrO doping showed the best cell differentiation and proliferation results. It was shown that SrO simultaneously improved mechanical properties of TCP such as compressive strength as well as biological properties via enhanced cell attachment and proliferation.
Shape Anisotropy, Exchange‐Coupling Interaction and Microwave Absorption of Hard/Soft Nanocomposite Ferrite Microfibers
Authors: Shen Xiangqian , Song Fuzhan , Xiang Jun , Liu Mingquan , Zhu Yongwei , Wang Yingde 
Published: August 2012

The nanocomposite BaFe12O19 (BFO)/Ni0.5Zn0.5Fe2O4 (NZFO)‐aligned microfibers with diameters 0.8–2 μm and high aspect ratios have been prepared by the gel precursor transformation process, where BFO is the hard magnetic phase and NZFO the soft magnetic phase. The nanocomposite binary ferrites are formed after the gel precursor calcined at 1000°C for 3 h. These nanocomposite aligned microfibers exhibit a clear shape anisotropy. Their magnetic difference observed at 297 and 77 K largely arises from the competition of exchange‐coupling interaction and thermal fluctuation on dipolar interaction. Consequently, their microwave absorption performance is largely influenced by the mass ratio of BFO/NZFO and specimen thickness. When the mass ratio (BFO:NZFO) is 7:3 and the specimen thickness is 3 mm, the minimum reflection loss (RL) reaches the optimized RL value of −35.5 dB at 12.4 GHz, with a wide absorption bandwidth (the RL value over −20 dB) from 9.1 to 15.7 GHz, which covers 80% of X‐band (8.2–12.4 GHz) and 59% of Ku‐band (12.4–18.0 GHz). This improvement in microwave absorption can be attributed to the exchange‐coupling interaction, shape anisotropy, interfacial polarization, and small size effect in nanocomposite microfibers.
Effect of Cation Doping on Mechanical Properties of Yttria Prepared by an Optimized Two‐Step Sintering Process
Authors: Khosroshahi Hadi Razavi , Ikeda Hiroyuki , Yamada Kazuhiro , Saito Noritaka , Kaneko Kenji , Hayashi Koichi , Nakashima Kunihiko 
Published: August 2012

Undoped Y2O3 and Y2O3 doped with either Si4+, Er3+, Yb3+, Al3+ or Ti4+ were fabricated by a two‐step sintering method. The concentration of each dopant cation (Si4+, Er3+, Yb3+, Al3+, and Ti4+) was adjusted so that the dopant:yttria molar ratio was 0.01 and the mechanical properties and the microstructures of each were characterized. The Vickers hardness and the bending strength of cation‐doped Y2O3 increased in the order Si4+, Er3+, Yb3+, Al3+, and Ti4+ paralleling increases in the relative density. Ti‐doped‐Y2O3 had the highest density and the highest strength. Transmission electron microscopy analysis revealed that Ti segregated to the grain boundaries of Y2O3. The two‐step sintering method was optimized to enhance the mechanical properties of Y2O3. As a consequence, Ti‐doped Y2O3 with the Ti4+:yttria molar ratio of 0.01 sintered at the optimized heating schedule was found to have a Vickers hardness and three‐point bending strength as high as 874 Hv and 307.0 MPa, respectively.
Piracetam‐induced changes on the brainstem auditory response in anesthetized juvenile rhesus monkeys (Macaca mulatta). Report of two clinical cases
Authors: Durand‐Rivera A. , Gonzalez‐Pina R. , Hernandez‐Godinez B. , Ibanez‐Contreras A. , Bueno‐Nava A. , Alfaro‐Rodriguez A. 
Published: August 2012

AbstractBackgroundWe describe two clinical cases and examine the effects of piracetam on the brainstem auditory response in infantile female rhesus monkeys (Macaca mulatta).ResultsWe found that the interwave intervals show a greater reduction in a 3‐year‐old rhesus monkey compared to a 1‐year‐old rhesus monkey.DiscussionIn this report, we discuss the significance of these observations.
Phacoemulsification considerations in nonhuman primates
Authors: Leiva Marta , Peña Teresa , Bayón Alejandro , de León Mónica , Morales Inmaculada 
Published: August 2012

AbstractPurposeTo describe the pre‐surgical, surgical, and post‐surgical considerations for phacoemulsification in non‐human primates.MethodsRecords of non‐human primates that underwent phacoemulsification by the Ophthalmology Services ofFHCV‐UABandHCV‐UM, between 2003 and 2009 were reviewed.ResultsFive primates were represented: one gorilla, one chimpanzee, one pygmy marmoset, one orangutan, and one chacma baboon. Three were males and two females, of a median age of 16.8 years. Four primates presented bilateral cataracts and one unilateral (n = 9 eyes). Cataracts were immature in two eyes, mature in four and hypermature in 3. One‐handed phacoemulsification was used in seven eyes and a two‐handed technique in 2. Intraocular lenses (IOLs), with optic powers between +19.0 and +21.5D, were implanted in the four large‐sized primates. All the primates were more active and social after the surgery.ConclusionPhacoemulsification in non‐human primates is highly successful and associated with an overall good visual outcome. Nevertheless, specific considerations should be applied in these species.
“Assessment of chronic sclerodermoid Graft‐versus‐Host Disease patients, using 20MHz high‐frequency ultrasonography and cutometer methods”
Authors: Osmola‐Mańkowska Agnieszka , Silny Wojciech , Dańczak‐Pazdrowska Aleksandra , Polańska Adriana , Olek‐Hrab Karolina , Sadowska‐Przytocka Anna , Żaba Ryszard , Jenerowicz Dorota 
Published: August 2012

AbstractThe development of an adverse graft‐versus‐host disease (GvHD) is a major complication of stem cell transplantations, which are widely used to cure increasing number of hematologic malignancies. Patients with chronic GvHD are at risk of joint contractures secondary to sclerodermatous skin changes. Several clinical scores or serologic markers have been used to assess skin sclerosis in scleroderma patients. Evaluation of sclerotic skin changes using biometric tools remains to be challenging. The purpose of this study was to illustrate and exemplify ultrasound measurement and measurement of skin elasticity of five chronic sclerodermoid GvHD patients. There is still a substantial lack of studies using objective and non‐invasive methods helpful in assessment of patients with skin involvement of GvHD. Although ultrasound is not the ideal method, it is worth emphasizing that it is still useful, non‐invasive, and repeatable device in monitoring patients suffering from GvHD. It should also be added, that it seems to be advisable to repeat USG examination at an interval of 3 months after the treatment. In addition, skin echogenicity may be a more sensitive parameter than skin thickness in assessment of cGvHD patients.
In vitro prediction of in vivo skin damage associated with the wiping of dry tissue against skin
Authors: Koenig David W. , Dvoracek Barb , Vongsa Rebecca 
Published: August 2012

BackgroundThe ideal gentle cleansing product is one that effectively removes soils while minimizing damage to the skin. Thus, measuring physical abrasion caused by cleansing tissues is critical to the continued development of gentle cleansing products. Current analysis of cleansing materials for skin gentleness is time consuming and requires expensive human subject testing. This report describes the development of a rapid and inexpensive bench assay for the assessment of skin abrasion caused by wiping.MethodsCoefficient of friction (COF) evaluations using bench methods were compared with results from clinical studies of repeated wiping and with confocal visualizations of excised skin. A Monitor/Slip and Friction™ instrument (model 32‐06; TMI, Amityville, NY, USA) was used to measure tissue friction on simulated skin (Vitro‐Skin®, N19‐5X; IMS, Milford, CT, USA). Clinical data from a 4‐day repetitive forearm wiping study measuring transepidermal water loss (TEWL) in 30 subjects was compared with results from the bench top assay. In addition, excised skin samples were also treated using the COF bench assay and examined using confocal microscopy to visualize stratum corneum damage caused by wiping.ResultsUsing the bench COF assay, we were able to distinguish between bath tissue codes by comparing average static friction value (ASFV) for the test codes, where lower ASFV indicated less abrasive tissue. The ASFV followed the same gentleness trend observed in the clinical study. Confocal microscopy of excised skin wiped with the same materials indicated stratum corneum damage consistent with the bench COF and clinical TEWL observations.ConclusionWe observed significant correlation between bench and clinical methods for measuring skin damage caused by wiping of skin with tissue. The bench method will facilitate rapid and inexpensive skin gentleness assessment of cleansing materials.
Visual pathway‐related horizontal reference plane for three‐dimensional craniofacial analysis
Authors: Kang Y. H. , Kim B. C. , Park K. R. , Yon J. Y. , Kim H. J. , Tak H. J. , Piao Z. , Kim M. K. , Lee S. H. 
Published: August 2012

Kang Y. H., Kim B. C., Park K. R., Yon J. Y., Kim H. J., Tak H. J., Piao Z., Kim M. K., Lee S. H. Visual pathway‐related horizontal reference plane for three‐dimensional craniofacial analysis  Orthod Craniofac Res 2012;15:245–254. © 2012 John Wiley & Sons A/SStructured AbstractAuthors –  Kang YH, Kim BC, Park KR, Yon JY, Kim HJ, Tak HJ, Piao Z, Kim MK, Lee SHObjectives –  To construct three‐dimensional (3D) horizontal reference planes based on visual pathway and to determine their stability and reliability by analyzing the structural patterns of normal and dysmorphology for 3D craniofacial analysis.Setting and Sample Population –  Thirty‐six subjects with maxillofacial dysmorphology and malocclusion, and eight normal controls.Materials and Methods Population –  On the 3D computed tomographic images of the subjects, the visual pathway‐based planes, including the orbital axis plane (OAP), visual axis plane (VAP), and the optical axis plane (OpAP), were constructed and evaluated.Results –  The OAP, but not the VAP and OpAP, showed the ideal relationship between the midsagittal and posterior maxillary plane, and properly described the different patterns of maxillofacial dysmorphology with craniofacial plane 1 of Delaire’s analysis and the occlusal plane.Conclusions –  The proposed visual pathway‐related horizontal reference planes, and in particular the OAP, seem to correctly express the visual axis and the position of the head in natural head position and can be used as a horizontal reference plane for the 3D analysis of craniofacial dysmorphology and anthropology.
Disentangling the role of edaphic variability, flooding regime and topography of Amazonian white‐sand vegetation
Authors: Damasco Gabriel , Vicentini Alberto , Castilho Carolina V. , Pimentel Tânia P. , Nascimento Henrique E.M. 
Published: August 2012

AbstractQuestionHow much variation in plant community structure and composition can be predicted using soil, flooding and topography in white‐sand vegetation of northern Amazonia?LocationBrazil, northern Amazon, Roraima State, Viruá National Park (01°46′34″ N, 61°02′06″ W).MethodsData from 17, 1‐ha permanent plots distributed across a 25 km2 landscape within the Viruá National Park, northern Amazonia, was used to assess the soil, flooding and topography effects on tree species composition, diversity and structure. Our analyses were based on 16 599 trees (DBH ≥ 1 cm) belonging to 303 species. Principal components analysis (PCA) was used to reduce the dimensionality of soil variables, whereas dimensionality of floristic composition assemblage was reduced using non‐metric multidimensional scaling (NMDS). Multiple regression analysis was used to test combined effects of soil, flooding and topography on floristic composition, diversity, basal area and tree density.ResultsDepending on the diameter class or the type of data (qualitative or quantitative), NMDS axis 1 explained 45–84% of total variation in species composition among plots. The first and second axes from PCA explained, respectively, 54.8% and 15.1% of the variation related to soil gradients. Soil texture and fertility (PCA axis 1) were the most important predictors of the variation in tree composition, diversity and structure for the three different size classes analysed (DBH ≥ 1 cm, 1 cm ≤ DBH ≤ 9.9 cm and DBH ≥ 10 cm). Overall, plots on more fertile clayey soils showed higher tree diversity and basal area than those on well‐drained sandy soils. Although flooding had little effect on the tree community, the water level was higher in high‐stature forests established on more clayey and fertile soils.ConclusionsEven though soils in the study area are predominantly sandy and oligotrophic, our findings highlight the importance of soil in structuring plant communities of seasonally flooded white‐sand vegetation at mesoscales in the Amazon Basin.
Changes in fire intensity have carry‐over effects on plant responses after the next fire in southern California chaparral
Authors: Moreno José M. , Torres Iván , Luna Belén , Oechel Walter C. , Keeley Jon E. 
Published: August 2012

AbstractQuestionDo variations in fire intensity within a stand determine changes in fire intensity and plant demographics in a subsequent fire?LocationSan Diego (CA, USA); chaparral dominated by Adenostoma fasciculatum (resprouter) and Ceanothus greggii (seeder).MethodsIn 2003, a wildfire burned a young (16‐yr‐old) stand containing a set of experimental plots burned in 1987 with various levels of fire intensity. In 2004, all the 1987 plots were sampled for Adenostoma survival and the recruitment of both species. Similar measures were carried out in the adjacent old (75‐yr) stand. Fire intensity in 2003 was estimated by a surrogate fire severity measure [minimum diameter of burned branches (branch diameter)].ResultsIn the young stand, branch diameter in 2003 was similar to the control plots in 1987, but lower than in the old stand. Fire intensity in 1987 did not significantly affect branch diameter in 2003. Survival of Adenostoma in the young stand was very low, much lower than after the 1987 burn and that in the old stand. Fire intensity in 1987 did not affect Adenostoma survival. Recruitment in Adenostoma increased, and in Ceanothus decreased, with increased fire intensity in 1987.ConclusionsWe demonstrate that there is a carry‐over effect of fire intensity across a whole fire cycle on plant recruitment of the two dominant species. The 2003 fire partially reversed the relative effects on recruitment caused by elevated fire intensity in 1987. Arguably, this effect was driven by the contrasted relationships of the two species to fire intensity. Adenostoma survival in the young stand was much lower in 2003 than in 1987, despite similar branch diameter, and was also lower than in the old stand, despite higher branch diameter in this case. The causes of such mortality are unknown.
Functional and structural studies of the disulfide isomerase DsbC from the plant pathogen Xylella fastidiosa reveals a redox‐dependent oligomeric modulation in vitro
Authors: Santos Clelton A. , Toledo Marcelo A. S. , Trivella Daniela B. B. , Beloti Lilian L. , Schneider Dilaine R. S. , Saraiva Antonio M. , Crucello Aline , Azzoni Adriano R. , Souza Alessandra A. , Aparicio Ricardo , Souza Anete P. 
Published: August 2012

Xylella fastidiosa is a Gram‐negative bacterium that grows as a biofilm inside the xylem vessels of susceptible plants and causes several economically relevant crop diseases. In the present study, we report the functional and low‐resolution structural characterization of the X. fastidiosa disulfide isomerase DsbC (XfDsbC). DsbC is part of the disulfide bond reduction/isomerization pathway in the bacterial periplasm and plays an important role in oxidative protein folding. In the present study, we demonstrate the presence of XfDsbC during different stages of X. fastidiosa biofilm development. XfDsbC was not detected during X. fastidiosa planktonic growth; however, after administering a sublethal copper shock, we observed an overexpression of XfDsbC that also occurred during planktonic growth. These results suggest that X. fastidiosa can use XfDsbC in vivo under oxidative stress conditions similar to those induced by copper. In addition, using dynamic light scattering and small‐angle X‐ray scattering, we observed that the oligomeric state of XfDsbC in vitro may be dependent on the redox environment. Under reducing conditions, XfDsbC is present as a dimer, whereas a putative tetrameric form was observed under nonreducing conditions. Taken together, our findings demonstrate the overexpression of XfDsbC during biofilm formation and provide the first structural model of a bacterial disulfide isomerase in solution.Structured digital abstract

XfDsbC and XfDsbC bind by x ray scattering (View Interaction: 1, 2)

XfDsbC and XfDsbC bind by molecular sieving (View interaction)

XfDsbC and XfDsbC bind by comigration in non denaturing gel electrophoresis (View interaction)

XfDsbC and XfDsbC bind by cross‐linking study (View Interaction: 1, 2)

XfDsbC and XfDsbC bind by dynamic light scattering (View Interaction: 1, 2)
The proline‐rich tetramerization peptides in equine serum butyrylcholinesterase
Authors: Biberoglu Kevser , Schopfer Lawrence M. , Tacal Ozden , Lockridge Oksana 
Published: August 2012

Soluble, tetrameric, plasma butyrylcholinesterase from horse has previously been shown to include a non‐covalently attached polyproline peptide in its structure. The polyproline peptide matched the polyproline‐rich region of human lamellipodin. Our goal was to examine the tetramer‐organizing peptides of horse butyrylcholinesterase in more detail. Horse butyrylcholinesterase was denatured by boiling, thus releasing a set of polyproline peptides ranging in mass from 1173 to 2098 Da. The peptide sequences were determined by fragmentation in MALDI‐TOF‐TOF and linear ion trap quadrupole Orbitrap mass spectrometers. Twenty‐seven polyproline peptides grouped into 13 families were identified. Peptides contained a minimum of 11 consecutive proline residues and as many as 21. Many of the peptides had a non‐proline amino acid at the N‐terminus. A search of the protein databanks matched peptides to nine proteins, although not all peptides matched a known protein. It is concluded that polyproline peptides of various lengths and sequences are included in the tetramer structure of horse butyrylcholinesterase. The function of these polyproline peptides is to serve as tetramer‐organizing peptides.Structured digital abstract

http://mint.bio.uniroma2.it/mint/search/interaction.do?interactionAc=MINT-7260296

BChE and BChE bind by comigration in non denaturing gel electrophoresis (View interaction)

BChE and BChE bind by comigration in sds page (View interaction)