bluuebunny/crossref_metadata_2025_split huggingface dataset:
TOURISM VILLAGE DEVELOPMENT DURING THE COVID-19 PANDEMIC CASE STUDY IN TEGALWANGI VILLAGE TOURISM VILLAGE DEVELOPMENT DURING THE COVID-19 PANDEMIC CASE STUDY IN TEGALWANGI VILLAGE
Authors: Pratama Muhamammad Hiqbal , Perwitasari Fitri Dian , Yuliananda Devi , Wicaksono Fredy , Bastoni  , Arisandi Bayu 
Published: April 2024

Students of Universitas Muhammadiyah Cirebon must take part in Student Work Lectures (KKM) as a form of their service to the community. One of the tasks of KKM group 23 in Tegalwangi village is to create a podcast that incorporates the Ngobras (Informal conversation) program about the tourist village. Chatting, talking, or talking casually is the definition of "ngobras". The theme of Ngobras was "A thriving tourist village in the era of industrial revolution 4.0", and the resource person was Mr. H. Nana Mulyana, head of the Cirebon Regency Disbudparpora. The purpose of the KKM "Ngobras" activity is to gather information about what needs to be prepared, considered, and developed by Tegalwangi village as a tourism village. The research location is Tegalwangi Village, Weru Subdistrict, Cirebon Regency, and descriptive methods are used. The results of this Ngobras KKM activity provide some input for Tegal Wangi Village to develop a tourist village, hoping that there will be follow-up by the village government.
SUMOylation of zebrafish transcription factor Zbtb21 affects its transcription activity
Authors: Fang Zhou , Deng Yun , Wang Haihong , Zhou Jun 
Published: April 2024

Background
Post-translational modification by Small Ubiquitin-like MOdifier (SUMO) is an important mechanism to regulate protein activity, protein stability, and localization of substrates. Zbtb21 is a zinc finger and BTB (Broad-complex, Tram-track and Bric à brac) domain-containing transcription factor. Bioinformatic prediction suggests several putative SUMOylated sites in Zbtb21 protein.

Methods
Two evolutionarily conserved lysine residues in Zbtb21 protein were mutated alone or in combination to disrupt the binding with SUMO molecules. Western blot and co-immunoprecipitation analyses were performed to detect the SUMOylation state of wild type and mutant Zbtb21 proteins, respectively. Luciferase reporter assays were conducted to evaluate their transcription activities. Meanwhile, immunofluorescence staining was carried out to show their sub-nuclear localizations. Finally, co-immunoprecipitation was performed to detect the interaction between Zbtb21 and its partners.

Results
Phylogenetically conserved lysines 419 and 845 of zebrafish Zbtb21 protein can be conjugated with SUMO molecules. SUMOylation does not affect the subcellular localization and protein stability of Zbtb21, as well as the interaction with Zbtb14 or Zbtb21. Nevertheless, luciferase reporter assays revealed that Zbtb21 is a dual-function transcription factor which exerts activation or repression effect on different promoters, and SUMOylation can modulate the transcriptional activity of Zbtb21 in regulating downstream target genes. Hence, Zbtb21 is identified as a novel substrate of SUMOylation, which would be important for its function.

Conclusions
Zebrafish Zbtb21 protein can be SUMOylated on lysines 419 and 845, which is evolutionary conserved. SUMOylation affects the dual role of Zbtb21 on transcription.
Molecular and Morphological Profiling of Rice Cultivars Using Hypervariable Microsatellite Markers and DUS Descriptors
Authors: Veeraghattapu Roja , Modugu Tushara , Badugu Krishnaveni , Jallu Pranaya , KalCluru Sudhamani , Chapara Rani 
Published: April 2024

Identification and protection of varieties is one of the most important tasks in the plant breeding programme. As the number of new varieties increasing year by year, there is a need to protect and maintain the purity of the varieties. DNA fingerprinting studies allows us to identify the plant varieties with high precision as it is not influenced by environment and other factors. In this context, the present study has been taken up for morphological and molecular profiling of 20 rice genotypes using 39 DUS descriptors and SSR markers. The field experiment was conducted during the year Kharif 2021 and Kharif 2022 at Agricultural Research Station, Bapatla and molecular profiling studies were conducted at RARS, Lam, Guntur. Significant variations were observed for 16 morphological traits at different growth stages, which are relatively more informative in the identification and characterization of rice genotypes. A total of 45 polymorphic SSR markers were used to screen 20 rice genotypes, which produced 336 alleles. The number of alleles produced by these markers ranged from 3 (RM1048) to 15 (RM124) with an average number of alleles of 7.46 per marker. The PIC values of these markers ranged from 0.54 (RM1048) to 0.92 (RM124) with an average PIC value of 0.75. SSR based molecular profiles were developed using 12 highly polymorphic SSR markers viz., RM495, RM6933, RM489, RM6006, RM3351, RM510, RM418, RM3215, RM105, RM6364, RM144 and RM2972 for discrimination of each genotype as well as easy identification.
Effect of Nitrogen, Zinc and Iron Fertilization on the Economic Yield of Wheat (Triticum aestivum. L)
Authors: Kumar Ajay , Kumar Mukesh , Kumar Pardeep , Qidwai Sidra , Yadav Vikas , Kaushik Shivam , Kumar Praveen 
Published: April 2024

The experiment was carried out entitled “Effect of nitrogen fertilizer and Ferti-fortification through zinc and iron on yield and economics of wheat (Triticum aestivum)” at the Crop Research Centre of Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, (U.P.) during rabi season of 2021-22 and 2022-23. The soil of the experimental area was sandy clay loam with low organic matter, available nitrogen, medium in available phosphorus, potassium, zinc, iron and slightly alkaline in reaction. The result of the experiment revealed that the maximum grain yield (52.83 q ha-1 and 51.44 q ha-1), straw yield (66.06 q ha-1 and 65.40 q ha-1), net returns (₹ 106,008 ha-1 and ₹ 112,155 ha-1) and B:C ratio (3.27 & 3.26) was recorded with recommended nitrogen (150 kg N ha-1) + foliar zinc (0.5%) + foliar iron (0.5 %) treatments during 2021-22 and 2022-23, respectively.
Taxonomic Distribution of Major Insect Pest of Rabi Season at Jhansi (U.P.) Using Solar Powered Light Traps
Authors: Chaturvedi Kushal , Vaishampayan Sanjay , Kumar Naveen , Rai Ankit , Akanksha . , Seervi Sitaram 
Published: April 2024

The experiment was conducted at Bundelkhand University, Jhansi (UP) during the period March and April, 2021 to study the taxonomic distribution of insect pest species collected in Solar powered Light Traps model SMV- 8 and SMV- 9 using low energy light source i.e. 7w UV LED. According to taxonomic analysis, 8 insect species were recorded and they belong to four orders and eight families. Based on the number of species collected, the order Lepidoptera had the biggest collection (42 per cent), followed by the order Coleoptera with 3 species (40 per cent). The pyraliidae family has the most insect pests (25 per cent), followed by the Scarabaeidae family (19 per cent).
Size Effect on Properties of Varistors Made From Zinc Oxide Nanoparticles Through Low Temperature Spark Plasma Sintering
Authors: Macary Léna Saint , Kahn Myrtil L. , Estournès Claude , Fau Pierre , Trémouilles David , Bafleur Marise , Renaud Philippe , Chaudret Bruno 
Published: April 2009

AbstractConditions for the elaboration of nanostructured varistors by spark plasma sintering (SPS) are investigated, using 8‐nm zinc oxide nanoparticles synthesized following an organometallic approach. A binary system constituted of zinc oxide and bismuth oxide nanoparticles is used for this purpose. It is synthesized at room temperature in an organic solution through the hydrolysis of dicyclohexylzinc and bismuth acetate precursors. Sintering of this material is performed by SPS at various temperatures and dwell times. The determination of the microstructure and the chemical composition of the as‐prepared ceramics are based on scanning electron microscopy and X‐ray diffraction analysis. The nonlinear electrical characteristics are evidenced by current–voltage measurements. The breakdown voltage of these nanostructured varistors strongly depends on grain sizes. The results show that nanostructured varistors are obtained by SPS at sintering temperatures ranging from 550 to 600 °C.
Synthesis, Mechanism, and Gas‐Sensing Application of Surfactant Tailored Tungsten Oxide Nanostructures
Authors: Pokhrel Suman , Simion Cristian E. , Teodorescu Valentin S. , Barsan Nicolae , Weimar Udo 
Published: April 2009

AbstractWidely applicable nonaqueous solution routes have been employed for the syntheses of crystalline nanostructured tungsten oxide particles from a tungsten hexachloride precursor. Here, a systematic study on the crystallization and assembly behavior of tungsten oxide products made by using the bioligand deferoxamine mesylate (DFOM) (product I), the two chelating ligands hexadecyltrimethylammoniumbromide (CTAB) (II) and poly(alkylene oxide) block copolymer (Pluronic P123) (III) is presented. The mechanistic pathways for the material synthesis are also discussed in detail. The tungsten oxide nanomaterials and reaction solutions are characterized by Fourier transform IR, 1H, and 13C NMR spectroscopies, powder X‐ray diffraction, scanning electron microscopy, transmission electron microscopy (TEM), high‐resolution TEM, and selected‐area electron diffraction. The indexing of the line pattern suggests WO3 is in its monoclinic structure with a = 0.7297 nm, b = 0.7539 nm, c = 0.7688 nm, and β‐i; = 90.91 °. The nanoparticles formed have various architectures, such as chromosomal shapes (product I) and slates (II), which are quite different from the mesoporous one (III) that has internal pores or mesopores ranging from 5 to 15 nm. The nanoparticles obtained from all the synthetic procedures are in the range of 40–60 nm. The investigation of the gas‐sensing properties of these materials indicate that all the sensors have good baseline stability and the sensors fabricated from material III present very different response kinetics and different CO detection properties. The possibility of adjusting the morphology and by that tuning the gas‐sensing properties makes the preparation strategies used interesting candidates for fabricating gas‐sensing materials.
Hierarchic Nanostructure for Auto‐Modulation of Material Release: Mesoporous Nanocompartment Films
Authors: Ji Qingmin , Acharya Somobrata , Hill Jonathan P. , Vinu Ajayan , Yoon Suk Bon , Yu Jong‐Sung , Sakamoto Kazutami , Ariga Katsuhiko 
Published: April 2009

AbstractThe preparation of mesoporous nanocompartment films composed of both hollow silica capsules and silica particles by using layer‐by‐layer (LbL) adsorption is described. The resultant nanocompartment films exhibit stepwise release of encapsulated water molecules without application of external stimuli. The hollow hierarchic pore structure of the silica capsules, including their internal void and mesoporous walls, is a key factor for the regulation and stepwise release of water, and is probably caused by the non‐equilibrated concurrent evaporation of material from the mesopore and capillary penetration into the mesopores. The number of release steps and rate of release can be tuned by variation of several parameters including water content, ambient temperature, layer multiplicity, and co‐adduct particle size. Application of the mesoporous nanocompartment films for the release of substances, including therapeutic agents and fragrances, indicates that the stepwise material release can be applied for a wide range of liquid substances. The films should lead to a novel material release system useful even for biomedical applications capable of controlled and sustained delivery of drug molecules.
The DRD3 rs6280 polymorphism and prevalence of tardive dyskinesia: A meta‐analysis
Authors: Tsai Huei‐Ting , North Kari E. , West Suzanne L. , Poole Charles 
Published: April 2009

AbstractTo elucidate a widely suspected but inconclusive association between rs6280 in the dopamine receptor 3 gene (DRD3) and prevalence of tardive dyskinesia (TD), we conducted a meta‐analysis of studies obtained in a systematic search of several bibliographic systems. We conducted several analyses of funnel plot asymmetry, overall heterogeneity, and study characteristics in analyses analogous to general, dominant and recessive inheritance models with the prevalence odds ratio (POR) as the measure of association. Thirteen eligible studies were identified with publication dates between 1997 and 2008. Evidence of funnel plot asymmetry was discerned in the dominant and general model analyses, but not in the recessive model analysis. Stratified analyses indicated that publication year, TD assessment method (Schooler–Kane criteria or other) and TD assessment frequency (single or repeated) were important study characteristics associated with heterogeneous PORs across studies. Studies conducted among patients with older age, fewer women or European (compared with Asian) ancestry reported stronger average PORs. Summary POR estimates under the dominant and general inheritance models were not warranted due to funnel plot asymmetry and heterogeneity. Under the recessive model, the summary estimate was POR = 0.93 (95% confidence interval: 0.70–1.23). We conclude that there is no or little association between DRD3 rs6280 polymorphisms and prevalence of TD. © 2009 Wiley‐Liss, Inc.
Brief communication: “Pathological” deformation in the skull of LB1, the type specimen of Homo floresiensis
Authors: Kaifu Yousuke , Baba Hisao , Kurniawan Iwan , Sutikna Thomas , Saptomo E. Wahyu , Jatmiko  , Awe Rokhus Due , Kaneko Tsuyoshi , Aziz Fachroel , Djubiantono Tony 
Published: April 2009

AbstractIf the holotype of Homo floresiensis, LB1, suffered from a severe developmental pathology, this could undermine its status as the holotype of a new species. One of the proposed pathological indicators that still remains untested is asymmetric distortion in the skull of LB1 (Jacob et al.: Proc Natl Acad Sci USA 103 (2006) 13421–13426). Here, we present evidence that LB1 exhibits antemortem craniofacial deformities that are consistent with posterior deformational (positional) plagiocephaly. This is a relatively common condition in modern people with no serious associated health problems and does not represent a severe developmental abnormality in LB1. Am J Phys Anthropol 2009. © 2009 Wiley‐Liss, Inc.
Extreme value analysis in biometrics
Authors: Hüsler Jürg 
Published: April 2009

AbstractWe review some approaches of extreme value analysis in the context of biometrical applications. The classical extreme value analysis is based on iid random variables. Two different general methods are applied, which will be discussed together with biometrical examples. Different estimation, testing, goodness‐of‐fit procedures for applications are discussed. Furthermore, some non‐classical situations are considered where the data are possibly dependent, where a non‐stationary behavior is observed in the data or where the observations are not univariate. A few open problems are also stated.
Faecal occult blood test-based screening programme with high compliance for colonoscopy has a strong clinical impact on colorectal cancer
Authors: Parente F , Marino B , DeVecchi N , Moretti R 
Published: April 2009

Abstract

Background
The results of a pilot colorectal cancer screening programme by biennial immunochemical faecal occult blood test (FOBT) are reported.

Methods
All residents aged between 50 and 69 years in the Italian province of Lecco were invited to have a FOBT. Those with a positive result were offered colonoscopy. FOBT uptake and compliance with colonoscopy were assessed. Detection rate and positive predictive value (PPV) for cancer and adenoma were calculated. Tumour stages were compared between screen-detected cancers and other colorectal cancers diagnosed within the target age group.

Results
Some 38 693 (49·6 per cent) of 78 083 individuals had a FOBT and 2392 (6·2 per cent) had a positive result. Colorectal cancer was diagnosed in 4·6 per cent and advanced adenoma in 32·7 per cent. PPVs were 4·0 per cent for cancer, 28·1 per cent for advanced adenoma and 36·6 per cent for any adenoma. There was a significant difference in incidence of stage III/IV disease between screened and non-screened cohorts. Compliance for colonoscopy was 92·0 per cent. Major determinants of compliance were age less than 59 years, female sex, high education level and non-manual work.

Conclusion
These results justify extension of colorectal cancer screening to other regions of Italy.
Enantioselective separation and determination of adrafinil and modafinil on Chiralcel OJ‐H column in rat serum and urine using solid‐phase extraction followed by HPLC
Authors: Rao R. Nageswara , Shinde Dhananjay D. 
Published: April 2009

AbstractA simple and rapid normal‐phase HPLC method for enantiospecific separation of a psychostimulant, adrafinil (ADL), and its metabolite modafinil (MDL) in rat serum and urine was developed. The separation was accomplished on a normal‐phase polysaccharide stationary phase Chiralcel OJ‐H using n‐hexane–ethanol (62:38 v/v) as a mobile phase at a flow rate of 1.0 mL/min. Detection was carried out at 225 nm using a photo diode array (PDA) detector. The elution order of the enantiomers was determined by a polarimeter connected in series with the PDA. ADL and its metabolite were recovered from rat serum and urine by solid phase extraction using Oasis HLB cartridges and the mean recoveries were ≥80%. The enantiomers were eluted within 15 min without any interference from endogenous substances. The calibration curves were linear (r2 > 0.998) in the concentration range of 1.20–500 µg/mL for ADL and MDL. The assay was specific, accurate, precise and reproducible (intra‐ and inter‐day precisions RSDs <7.2%). ADL in rat serum was stable over three freeze–thaw cycles at ambient temperature for 4 h. The method was successfully applied to pharmacokinetic studies of adrafinil after an oral administration to rats. Copyright © 2009 John Wiley & Sons, Ltd.
Rapid separation and identification of 54 major constituents in Buyang Huanwu decoction by ultra‐fast HPLC system coupled with DAD‐TOF/MS
Authors: Liu E‐Hu , Qi Lian‐Wen , Peng Yong‐Bo , Cheng Xiao‐Lan , Wu Qian , Li Ping , Li Chang‐Yin 
Published: April 2009

AbstractBuyang Huanwu Decoction (BYHWD), is a well‐known traditional Chinese preparation consisting of Radix Astragali, Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong, Radix paeoniae Rubra, Flos Carthami, Semen Persicae and Lumbricus. An ultra‐fast high‐performance liquid chromatography (HPLC) method coupled with diode array detection (DAD) and electrospray ionization time‐of‐flight mass spectrometry (ESI‐TOF/MS) has been developed for rapid separation and structural identification of constituents in BYHWD. Using an ultra‐fast HPLC system with short columns (4.6 × 50 mm, 1.8 µm), the total analysis time for this complex prescription is less than 30 min. With various fragmentor voltages in TOF/MS, accurate mass measurements (less than 5 ppm error) for molecular ions and characteristic fragment ions could represent reliable identification criteria for these compounds. Fifty‐four major constituents from BYHWD sample, including four C‐glycosyl quinochalcones, four flavonoid O‐glycosides, sixteen isoflavones, six monoterpene glycosides, eight saponins, four organic acids and five amino acids, were identified or tentatively characterized based on their retention times, DAD and TOF/MS data. All the compounds were further assigned in the seven individual crude drugs. In conclusion, the ultra‐fast HPLC with DAD‐TOF/MS is a highly useful and efficient technique to separate and identify constituents in complex matrices of herbal medicines or preparations. Copyright © 2009 John Wiley & Sons, Ltd.
Determination of risperidone and 9‐Hydroxyrisperidone using HPLC, in plasma of children and adolescents with emotional and behavioural disorders
Authors: Jones Tanya , Van Breda Karin , Charles Bruce , Dean Angela J. , McDermott Brett M. , Norris Ross 
Published: April 2009

AbstractA simple, rapid, selective, accurate and precise method is described for the determination of risperidone and its active metabolite, 9‐hydroxyrisperidone, in plasma using a chemical derivative of risperidone (methyl‐risperidone) as the internal standard. The sample workup involved a single‐step extraction of 1 mL plasma, buffered to pH 10, with heptane–isoamyl alcohol (98:2 v/v), then evaporation of the heptane phase and reconstitution of the residue in mobile phase. HPLC separation was carried out at on C18column using a mobile phase of 0.05 mdipotassium hydrogen orthophosphate (containing 0.3% v/v triethylamine) adjusted to pH 3.7 with orthophosphoric acid (700 mL), and acetonitrile (300 mL). Flow rate was 0.6 mL/min and the detection wavelength was 280 nm. Retention times were 2.6, 3.7 and 5.8 min for 9‐hydroxy risperidone, risperidone and the internal standard, respectively. Linearity in spiked plasma was demonstrated from 2 to 100 ng/mL for both risperidone and 9‐hydroxyrisperidone (r≥ 0.999). Total imprecision was less than 13% (determined as co‐efficient of variation) and the inaccuracy was less than 12% at spiked concentrations of 5 and 80 ng/mL. The limit of detection, determined as three times the baseline noise, was 1.5 ng/mL. Clinical application of the assay was demonstrated for analysis of post‐dose (0.55–4.0 mg/day) samples from 28 paediatric patients (aged 6.9–17.9 years) who were taking risperidone orally for behavioural and emotional disorders. Copyright © 2009 John Wiley & Sons, Ltd.
Metabolite analysis in Curcuma domestica using various GC‐MS and LC‐MS separation and detection techniques
Authors: Herebian Diran , Choi Jeong‐Heui , Abd El‐Aty A. M. , Shim Jae‐Han , Spiteller Michael 
Published: April 2009

AbstractThe metabolic profile of polar (methanol) and non‐polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC‐FID, GC‐MS, HR‐GC‐MS and analytical HPLC‐ESI‐MS/MS by means of LTQ‐Orbitrap technology. The major non‐volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS‐based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives. Copyright © 2009 John Wiley & Sons, Ltd.
Computer‐controlled air‐conditioning experimental apparatus
Authors: Abu‐Mulaweh Hosni I. 
Published: April 2009

AbstractA portable experimental setup for demonstrating air‐conditioning processes was designed, developed and constructed by a multidisciplinary capstone senior design team. This experimental apparatus is currently being used to demonstrate to undergraduate students air‐conditioning processes such as cooling, heating, and humidification. The level of cooling, heating, or humidification is being monitored and controlled using a PC‐based control system which consists of LabVIEW and data acquisition unit. This article provides details about the integration of the electrical/electronic component and the control system. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 18: 458–468, 2010; View this article online at wileyonlinelibrary.com; DOI 10.1002/cae.20185