bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the effect of millennial tourists’ accommodation service experiences using structural equation modeling techniques: an emerging economy context
Authors: Hossain Md. Shakhawat , Hossain Md. Alamgir , Masud Abdullah Al , Hossain Mohammad Sabbir 
Published: June 2024

Purpose The purpose of this study is to investigate the role of millennial tourists’ accommodation service experiences (ASEs) on their satisfaction, word-of-mouth (WOM) and revisit intentions (RIs) in an emerging economy.Design/methodology/approach A survey instrument was used to collect cross-sectional data from millennial tourists, and the 282 valid datasets were analyzed using structural equation modeling.Findings The results demonstrated that ASE had a beneficial impact on satisfaction and WOM but not on RI. Significant positive associations between tourist satisfaction, WOM and RI were also discovered. Additionally, WOM research sheds new light on how the ASE of millennial tourists affects their satisfaction, WOM and propensity to return. Furthermore, results show that WOM intentions and satisfaction mediate the relationship between ASE and RIs.Originality/value The study presents a unique research context, the application of advanced statistical techniques and the comprehensive investigation of key outcome variables in the context of millennial tourists’ ASEs in an emerging economy. This study contributes significantly to the body of knowledge in the field of tourism research, aiming to meet long-term goals in a sustainable way for the hospitality industry operators by integrating ASE, satisfaction, WOM and RI. Additionally, the study presents the mediating role of WOM and satisfaction in the millennial tourists’ emerging country context.
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila
Authors: D'Ermo Giulia , Audebert Stéphane , Camoin Luc , Planer‐Friedrich Britta , Casiot‐Marouani Corinne , Delpoux Sophie , Lebrun Régine , Guiral Marianne , Schoepp‐Cothenet Barbara 
Published: June 2024

AbstractThe metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sulphur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur‐oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography‐inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome c551/c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551/c5 and HiPIP III directing electrons towards the Reaction Centre.
From ubiquity to specificity: The diverse functions of bacterial thioredoxin systems
Authors: Anjou Cyril , Lotoux Aurélie , Morvan Claire , Martin‐Verstraete Isabelle 
Published: June 2024

AbstractThe thioredoxin (Trx) system, found universally, is responsible for the regeneration of reversibly oxidized protein thiols in living cells. This system is made up of a Trx and a Trx reductase, and it plays a central role in maintaining thiol‐based redox homeostasis by reducing oxidized protein thiols, such as disulfide bonds in proteins. Some Trxs also possess a chaperone function that is independent of thiol‐disulfide exchange, in addition to their thiol‐disulfide reductase activity. These two activities of the Trx system are involved in numerous physiological processes in bacteria. This review describes the diverse physiological roles of the Trx system that have emerged throughout bacterial evolution. The Trx system is essential for responding to oxidative and nitrosative stress. Beyond this primary function, the Trx system also participates in redox regulation and signal transduction, and in controlling metabolism, motility, biofilm formation, and virulence. This range of functions has evolved alongside the diversity of bacterial lifestyles and their specific constraints. This evolution can be characterized by the multiplication of the systems and by the specialization of cofactors or targets to adapt to the constraints of atypical lifestyles, such as photosynthesis, insect endosymbiosis, or spore‐forming bacteria.
Glucose evaluation and management in the ICU (GEM‐ICU): Protocol for a bi‐centre cohort study
Authors: Grigonyte‐Daraskeviciene Milda , Møller Morten Hylander , Kaas‐Hansen Benjamin Skov , Bestle Morten Heiberg , Nielsen Christian Gantzel , Perner Anders 
Published: June 2024

AbstractIntroductionHyperglycaemia is common in intensive care unit (ICU) patients. Glycaemic monitoring and effective glycaemic control with insulin are crucial in the ICU to improve patient outcomes. However, glycaemic control and insulin use vary between ICU patients and hypo‐ and hyperglycaemia occurs. Therefore, we aim to provide contemporary data on glycaemic control and management, and associated outcomes, in adult ICU patients. We hypothesise that the occurrence of hypoglycaemia in acutely admitted ICU patients is lower than that of hyperglycaemia.MethodsWe will conduct a bi‐centre cohort study of 300 acutely admitted adult ICU patients. Routine data will be collected retrospectively at baseline (ICU admission) and daily during ICU stay up to a maximum of 30 days. The primary outcome will be the number of patients with hypoglycaemia during their ICU stay. Secondary outcomes will be occurrence of severe hypoglycaemia, occurrence of hyperglycaemia, time below blood glucose target range, time above target range, all‐cause mortality at Day 30, number of days alive without life support at Day 30 and number of days alive and out of hospital at Day 30. Process outcomes include the number of in‐ICU days, glucose measurements (number of measurements and method) and use of insulin (including route of administration and dosage). All statistical analyses will be descriptive.ConclusionsThis cohort study will provide a contemporary overview of glucose evaluation and management practices in adult ICU patients and, thus, highlight potential areas for improvement through future clinical trials in this area.
Aging‐induced short‐chain acyl‐CoA dehydrogenase promotes age‐related hepatic steatosis by suppressing lipophagy
Authors: Deng Dan , Yang Shanshan , Yu Xiaoqian , Zhou Ruixue , Liu Yin , Zhang Hongmei , Cui Daxin , Feng Xingrong , Wu Yanting , Qi Xiaocun , Su Zhiguang 
Published: June 2024

AbstractHepatic steatosis, the first step in the development of nonalcoholic fatty liver disease (NAFLD), is frequently observed in the aging population. However, the underlying molecular mechanism remains largely unknown. In this study, we first employed GSEA enrichment analysis to identify short‐chain acyl‐CoA dehydrogenase (SCAD), which participates in the mitochondrial β‐oxidation of fatty acids and may be associated with hepatic steatosis in elderly individuals. Subsequently, we examined SCAD expression and hepatic triglyceride content in various aged humans and mice and found that triglycerides were markedly increased and that SCAD was upregulated in aged livers. Our further evidence in SCAD‐ablated mice suggested that SCAD deletion was able to slow liver aging and ameliorate aging‐associated fatty liver. Examination of the molecular pathways by which the deletion of SCAD attenuates steatosis revealed that the autophagic degradation of lipid droplets, which was not detected in elderly wild‐type mice, was maintained in SCAD‐deficient old mice. This was due to the decrease in the production of acetyl‐coenzyme A (acetyl‐CoA), which is abundant in the livers of old wild‐type mice. In conclusion, our findings demonstrate that the suppression of SCAD may prevent age‐associated hepatic steatosis by promoting lipophagy and that SCAD could be a promising therapeutic target for liver aging and associated steatosis.
Morphology of sweat glands in the skin of Saimaa (Pusa hispida saimensis) and Baltic ringed (Pusa hispida botnica) seals
Authors: Itkonen Laura , Nihtilä Heini , Laakkonen Juha 
Published: June 2024

AbstractThe endangered Saimaa ringed seal (Pusa hispida saimensis) is an endemic freshwater subspecies inhabiting Lake Saimaa in Finland. The Baltic ringed seal (Pusa hispida botnica) inhabits the brackish Baltic Sea, which is almost entirely landlocked. Recent research shows that Saimaa and Baltic ringed seals may be genetically even further apart from each other than from other ringed seal subspecies. We documented histologically the integument microstructure of Saimaa and Baltic ringed seals to determine whether the geographic and genetic isolation was manifested as variation in the integument microstructure of these subspecies adapted to icy aquatic environments. The skin structures of these subspecies were similar to those of other phocids. The association of the sweat glands with hair follicles in both subspecies suggested that they were small apocrine sweat glands described previously in terrestrial or aquatic mammals. None of the apocrine glands had large lumina, and some of the ducts were relatively straight and short. Further studies analysing the mode of secretion, for example, apocrine versus eccrine, in the sweat glands are necessary to confirm the types of sweat glands in seals.
In vitro modulation the Notch pathway by piperine: A therapeutic strategy for docetaxel‐resistant and non‐resistant prostate cancer
Authors: Wang Rui‐tao , Liu Hao‐en , Sun Hui‐yuan 
Published: June 2024

AbstractDocetaxel (DTX) resistance poses a significant challenge in the treatment of prostate cancer (PCa), often leading to chemotherapy failure. This study investigates the ability of piperine, a compound derived from black pepper, to enhance the sensitivity of PCa cells to DTX and elucidates its underlying mechanism. We established a DTX‐resistant PCa cell line, DU145/DTX, to conduct our experiments. Through a series of assays, including MTT for cell viability, flow cytometry for apoptosis, Transwell for cell migration and invasion, and western blot for protein expression analysis, we assessed the effects of piperine on these cellular functions and on the Notch signaling pathway components. Our results demonstrated that we successfully established the DTX‐resistant PCa cell line DU145/DTX. Piperine effectively decreased the viability of both DU145 and its DTX‐resistant counterpart, DU145/DTX, in a concentration and time‐dependent manner when used alone and in combination with DTX. Notably, piperine also induced apoptosis and reduced the migration and invasion capabilities of these cells. At the molecular level, piperine down‐regulated the Notch pathway by inhibiting Notch1 and Jagged1 signaling, as well as reducing the expression of downstream effectors Hey1 and hes family bHLH transcription factor 1. The study concludes that piperine's ability to modulate the Notch signaling pathway and induce apoptosis highlights its potential as a complementary treatment for DTX‐resistant PCa, paving the way for the use of traditional Chinese medicinal compounds in modern oncology treatment strategies.
A New Community‐to‐Hospital Specialist Otology Pathway Using Telemedicine: A Pilot Study of 50 Patients
Authors: Patel Ankit , Forde Cillian , Doal Suneal , Patel Jay , Clare Dawn , Burslem Michael , Manjaly Joseph G. , Mehta Nishchay 
Published: June 2024

ABSTRACTIntroductionPathway innovation using smartphone otoscopy and tablet‐based audiometry technologies to deliver ear and hearing services via trained audiologists may improve efficiency of the service. An ENT‐integrated‐community‐ear service (ENTICES—combining community audiology management, remote ENT review and novel technologies) was piloted. We aimed to assess the efficiency and safety of ENTICES.MethodENTICES was a community‐based and audiologist‐led pathway. Patients with otological symptoms were self‐referred to this service. Smartphone otoscopy and tablet‐based audiograms were performed. Two otologists reviewed all decisions made in the community by audiologists based on video‐otoscopy, hearing tests and chart reviews. Data on the first 50 consecutive new patients attending either consultant‐led hospital otology clinics (HOC), audiologist‐led hospital advanced audiology diagnostics (AAD) or ENTICES clinics were collected between 1 August 2021 and 31 December 2021. Data were collected through chart reviews and questionnaires to compare the three pathways with respect to efficiency, patient satisfaction, technology utility and safety.ResultsNo audiology‐led ENTICES decisions were amended by hospital otologists following remote review. Remote review of video‐otoscopy with history was sufficient for a diagnosis in 80% of cases. Adding hearing tests and standardised history increased the diagnostic yield to 98%. Patient satisfaction scores showed 100% service recommendation. The cost per patient, per visit, was £83.36, £99.07 and £69.72 for AAD, HOC or ENTICES, respectively.ConclusionENTICES provides a safe ear and hearing service that patients rated highly. Thirty‐two per cent of hospital otology patients were eligible for this service. For those patients, ENTICES is 20% more cost‐effective and can reduce the number of clinic visits by up to 60% compared with HOC.
Retrospective study on pomalidomide‐PACE as a salvage regimen in aggressive relapsed and refractory multiple myeloma
Authors: Gezer Deniz , Nogueira Melanie Schmitt , Kirschner Martin , Brümmendorf Tim H. , Müller‐Tidow Carsten , Goldschmidt Hartmut , Raab Marc S. , Giesen Nicola 
Published: June 2024

AbstractObjectivesDespite major advances in treatment options for multiple myeloma (MM), patients refractory to the main drug classes and those with aggressive, especially extramedullary disease, still face a dismal outcome. For these patients, effective therapeutic options are urgently warranted.MethodsIn this retrospective study, we report on the safety and efficacy of the intensive combination regimen of pomalidomide plus cisplatin, doxorubicin, cyclophosphamide, and etoposide (Pom‐PACE) in patients with relapsed refractory MM (RRMM) or plasma cell leukemia (PCL). A study population of 20 consecutive patients treated with Pom‐PACE at two academic centers was included for analysis. All patients had to have a confirmed relapse according to International Myeloma Working Group criteria and adequate organ function prior to the start of therapy. Data were collected by reviewing medical charts. Exploratory analyses were performed with regard to efficacy and safety.ResultsPatients were heavily pretreated with a median number of four prior therapies (range: 1–10). All patients were exposed to immunomodulators, proteasome inhibitors, and alkylating agents, 80% were double‐class refractory, 40% were triple‐class refractory. Extramedullary MM or PCL were present in 15 patients (75%). Overall response rate (ORR) was 68%, with 31% achieving at least a very good partial response. Responses were achieved rapidly with an ORR of 64% after one cycle. Median progression‐free survival was 8.9 months (0.92–not reached [NR]) and median overall survival was 11.8 months (3–40.6). Pom‐PACE was associated with significant toxicity. All evaluable patients experienced Grade 4 hematological toxicity. However, no treatment related mortality was observed.ConclusionPomalidomide‐PACE was able to induce rapid responses in heavily pretreated, aggressive RRMM with a manageable toxicity profile and therefore offers an effective salvage regimen and a potential bridging strategy to further treatment options such as chimeric antigen receptor T‐cell therapy.
The challenge of estimating global termite methane emissions
Authors: Law Stephanie J. , Allison Steven D. , Davies Andrew B. , Flores‐Moreno Habacuc , Wijas Baptiste J. , Yatsko Abbey R. , Zhou Yong , Zanne Amy E. , Eggleton Paul 
Published: June 2024

AbstractMethane is a powerful greenhouse gas, more potent than carbon dioxide, and emitted from a variety of natural sources including wetlands, permafrost, mammalian guts and termites. As increases in global temperatures continue to break records, quantifying the magnitudes of key methane sources has never been more pertinent. Over the last 40 years, the contribution of termites to the global methane budget has been subject to much debate. The most recent estimates of termite emissions range between 9 and 15 Tg CH4 year−1, approximately 4% of emissions from natural sources (excluding wetlands). However, we argue that the current approach for estimating termite contributions to the global methane budget is flawed. Key parameters, namely termite methane emissions from soil, deadwood, living tree stems, epigeal mounds and arboreal nests, are largely ignored in global estimates. This omission occurs because data are lacking and research objectives, crucially, neglect variation in termite ecology. Furthermore, inconsistencies in data collection methods prohibit the pooling of data required to compute global estimates. Here, we summarise the advances made over the last 40 years and illustrate how different aspects of termite ecology can influence the termite contribution to global methane emissions. Additionally, we highlight technological advances that may help researchers investigate termite methane emissions on a larger scale. Finally, we consider dynamic feedback mechanisms of climate warming and land‐use change on termite methane emissions. We conclude that ultimately the global contribution of termites to atmospheric methane remains unknown and thus present an alternative framework for estimating their emissions. To significantly improve estimates, we outline outstanding questions to guide future research efforts.
Global Primary Antibiotic Resistance Rate of Helicobacter pylori in Recent 10 years: A Systematic Review and Meta‐Analysis
Authors: Yu Yanhui , Xue Jing , Lin Fangbing , Liu Daming , Zhang Wen , Ru Shuying , Jiang Feng 
Published: June 2024

ABSTRACTIntroductionDue to irregular antibiotic use, the rate of antibiotic resistance to Helicobacter pylori (H. pylori) is increasing and varies from region to region. Therefore, for the purpose of further clarifying the changes in antibiotic resistance rates nowadays, we conducted a systematic review and meta‐analysis to update and assess the 10‐year trend of primary H. pylori antibiotic resistance rate to the commonly prescribed antibiotics worldwide.Materials and MethodsAccording to the PRISMA statement, we systematically searched electronic databases for studies that assessed rates of H. pylori resistance to clarithromycin, metronidazole, levofloxacin, amoxicillin, or tetracycline published from 2013 to 2023. AHRQ was adopted to estimate methodological quality and publication bias in the included studies, and statistical analysis was performed using Stata 17.0.ResultsWe identified 163 studies, comprising 47,002 isolates from 36 countries. The meta‐analysis showed that the primary antibiotic resistance rate of H. pylori varied widely among antibiotics. Subgroup analysis showed higher rates of antibiotic resistance in the adult population than in children, and a general trend of increased resistance was observed from 2013 to 2023. There was considerable heterogeneity (I2 > 75%) among all analyses, which may be due to high variability in resistance rates across the global regions.ConclusionsResistance of H. pylori to antibiotics has reached alarming levels worldwide, which has a great effect on the efficacy of treatment. Local surveillance networks are required to select appropriate eradication regimens for each region.
Exploring the Experiences of Living With the Post‐COVID Syndrome: A Qualitative Study
Authors: Kalfas Michail , Jolley Caroline , Hart Nicholas , Rafferty Gerrard F. , Duncan Emma L. , Nicholson Timothy , Ashworth Mark , Brewin Debbie , Barrett Barbara , Witard Oliver C. , Ridge Damien , Chalder Trudie 
Published: June 2024

ABSTRACTIntroductionMany people experience persistent symptoms for more than 12 weeks following SARS‐CoV‐2 infection, which is known as post‐COVID‐19 condition (PCS) or Long COVID (LC). PCS can impair people's quality of life and daily functioning. However, there is a lack of in‐depth research exploring the PCS patient journey, as well as gendered aspects of patients' experiences.MethodsNineteen semi‐structured qualitative interviews were conducted with people living with PCS in the United Kingdom (13 women, 6 men). Interviews were transcribed verbatim and analysed inductively using reflexive thematic analysis.ResultsFive main themes were identified: ‘Symptom dismissal’, ‘Lack of information and support’, ‘Life before and after Long COVID’, ‘Psychological impact’ and ‘Acceptance’. A shift overtime to self‐management of symptoms was evident. These themes represent different stages of patients' PCS journey. Narratives indicated that women highlighted dismissal by healthcare professionals (HCPs), which was not as prominent in men's narratives. In addition, women went into more detail about the psychological impact of PCS compared to men.ConclusionWomen with PCS reported symptom dismissal by HCPs, which may have delayed their diagnosis and negatively affected their well‐being. We were not able to explore the experiences of people from non‐conforming gender groups. Raising awareness of these issues among HCPs, particularly general practitioners, could improve patient care in PCS.Patient or Public ContributionPatient and public involvement consisted of people who took part in the interviews and commented on the themes' interpretation and study conclusions.
Application of Johnson's approximation in finite element modeling for electric field‐dependent materials
Authors: D'Silva Green Rassell C. , Dale Graham , McLaughlin Garry , Strawhorne Maureen , Sinclair Derek C. , Dean Julian S. 
Published: June 2024

AbstractJohnson's approximation is implemented in a finite element code to simulate the electric field dependence of a core–shell microstructure material. We show how the microstructure, based here on a 50:50 volume fraction, influences the measured effective permittivity as a function of applied voltage. Using a Johnson's parameter of β = 1.0 × 1010 Vm5/C3, verified from commercial BaTiO3‐based multilayer ceramic capacitors (MLCC), we show how the microstructure and the difference in core and shell conductivities alter the local fields generated and how this influences the voltage dependence of the effective permittivity. Systems that comprise a conductive core‐like material surrounded by a resistive shell experience little or modest voltage dependence due to the shell material providing shielding to large electric fields within the cores. Conversely, if the core material is more resistive than the shell material, substantial voltage dependence occurs with simulations showing over a 50% decrease in the effective permittivity. These simulations give improved understanding of voltage dependence and provide a method to help guide the design of future materials for MLCCs with improved performance.