bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex Differences in the Global Prevalence of Nonsuicidal Self-Injury in Adolescents
Authors: Moloney Fiona , Amini Jasmine , Sinyor Mark , Schaffer Ayal , Lanctôt Krista L. , Mitchell Rachel H.B. 
Published: June 2024

ImportanceNonsuicidal self-injury (NSSI) is a strong predictor of suicide attempts. The prevalence of NSSI has been increasing among female adolescents in North America and Europe, but less is known about trends in other geographical regions.ObjectiveTo examine sex differences in the prevalence of NSSI among adolescents within and between geographical regions.Data SourcesMEDLINE and PsycINFO were searched using the keywords adolescents, self-injury, sex factors, and synonyms for articles published in English between January 1, 2000, and May 10, 2022.Study SelectionStudies were included if they presented original data (any study design), included adolescents aged 10 to 19 years, reported results stratified by sex, and explicitly defined self-injury as behaviors occurring without suicidal intent.Data Extraction and SynthesisThis meta-analysis was registered with PROSPERO and conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses. Articles were assessed for quality by 2 independent coders (F.M. and J.A.). A random-effects model was used to calculate prevalence. Data were analyzed from July 2022 to April 2023.Main Outcomes and MeasuresThe prevalence of NSSI in male and female adolescents within and between regions was the main outcome. Odds ratios (OR) with 95% CIs were calculated for community samples.ResultsEight hundred and two studies were screened, and 38 were included (266 491 participants). Across 17 countries, the pooled prevalence of NSSI was 17.7% (female:male OR, 1.60; 95% CI, 1.29-1.98). NSSI was twice as prevalent among female adolescents compared with male adolescents in North America (OR, 2.49; 95% CI, 2.16-2.86) and Europe (OR, 2.08; 95% CI, 1.69-2.58), but not in Asia (OR, 1.00; 95% CI, 0.71-1.41).Conclusions and RelevanceIn this meta-analysis of sex differences in global prevalence of NSSI, the female predominance of NSSI observed among adolescents in North America and Europe aligned with rising rates of suicide in these populations. The comparable prevalence of NSSI among male and female adolescents in Asia also aligned with the lower male-to-female suicide ratio compared with other countries. More research is needed to characterize regional (and potentially cultural) sex differences among adolescents with NSSI to prevent and treat the behavior and to understand the possible interplay with corresponding regional trends in suicide.
Clinical Practice Guideline for Red Blood Cell Transfusion Thresholds in Very Preterm Neonates
Authors: Deschmann Emöke , Dame Christof , Sola-Visner Martha C. , Fustolo-Gunnink Susanna F. , Guyatt Gordon H. , Patel Ravi Mangal , Stanworth Simon J. ,   Neonatal Transfusion Network, New Helen , Lopriore Enrico , Roehr Charles Christoph , Coutinho Estela , Croitoru Corina , Nagy-Bonnard Livia , Matthäus Valerie , Nestaas Eirik , Buonocore Giuseppe , de Luca Daniele , Sanchez-Luna Manuel , Di Nardo Matteo , Huq Saifee Nabiha , Josephson Cassandra , Huijssen-Huisman Elise J. , Lieberman Lani , Orenstein Evan 
Published: June 2024

ImportanceRed blood cell (RBC) transfusion is a common medical intervention to treat anemia in very preterm neonates; however, best transfusion practices, such as thresholds, remain uncertain.ObjectiveTo develop recommendations for clinicians on the use of RBC transfusions in very preterm neonates.Evidence ReviewAn international steering committee reviewed evidence from a systematic review of 6 randomized clinical trials (RCTs) that compared high vs low hemoglobin-based or hematocrit-based transfusion thresholds. The steering committee reached consensus on certainty-of-evidence ratings and worked with a panel from stakeholder organizations on reviewing the evidence. With input from parent representatives and the stakeholder panel, the steering committee used the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach to develop recommendations.FindingsA systematic review of 6 RCTs encompassing 3483 participants (1759 females [51.3%]; mean [SD] age range, 25.9-29.8 [1.5-3.0] weeks) was used as the basis of the recommendations. The ranges for higher hemoglobin concentration (liberal) vs lower hemoglobin concentration (restrictive) threshold study arms were similar across the trials. However, specific thresholds differed based on the severity of illness, which was defined using variable criteria in the trials. There was moderate certainty of evidence that low transfusion thresholds likely had little to no difference in important short-term and long-term outcomes. The recommended hemoglobin thresholds varied on the basis of postnatal week and respiratory support needs. At postnatal weeks 1, 2, and 3 or more, for neonates on respiratory support, the recommended thresholds were 11, 10, and 9 g/dL, respectively; for neonates on no or minimal respiratory support, the recommended thresholds were 10, 8.5, and 7 g/dL, respectively (to convert hemoglobin to grams per liter, multiply by 10.0).Conclusions and RelevanceThis consensus statement recommends a restrictive RBC transfusion strategy, with moderate certainty of evidence, for preterm neonates with less than 30 weeks’ gestation.
The neural substrates of social cognition deficits in newly diagnosed multiple sclerosis patients
Authors: Ziccardi Stefano , Genova Helen , Colato Elisa , Guandalini Maddalena , Tamanti Agnese , Calabrese Massimiliano 
Published: June 2024

AbstractObjectiveCognitive and affective symptoms in multiple sclerosis (MS) can be independently impaired and have different pathways of progression. Cognitive alterations have been described since the earliest MS stages; by contrast, the social cognition (SC) domain has never been investigated in the first year from MS diagnosis. We aimed to evaluate SC and unravel its neural bases in newly diagnosed MS patients.MethodsSeventy MS patients underwent at diagnosis a 3 T‐MRI and a neuropsychological/SC assessment (median time between diagnosis and MRI/cognitive evaluation = 0 months). We tested two matched reference samples: 31 relapsing–remitting MS patients with longer course (mean ± SD disease duration = 7.0 ± 4.5 years) and 38 healthy controls (HCs). Cortical thicknesses (CTh) and volumes of brain regions were calculated.ResultsNewly diagnosed MS patients performed significantly lower than HCs in facial emotion recognition (global: p < 0.001; happiness: p = 0.041, anger: p = 0.007; fear: p < 0.001; disgust: p = 0.004) and theory of mind (p = 0.005), while no difference was found between newly diagnosed and longer MS patients. Compared to lower performers, higher performers in facial emotion recognition showed greater volume of amygdala (p = 0.032) and caudate (p = 0.036); higher performers in theory of mind showed greater CTh in lingual gyrus (p = 0.006), cuneus (p = 0.024), isthmus cingulate (p = 0.038), greater volumes of putamen (p = 0.016), pallidum (p = 0.029), and amygdala (p = 0.032); patients with higher empathy showed lower cuneus CTh (p = 0.042) and putamen volume (p = 0.007).InterpretationsSC deficits are present in MS patients since the time of diagnosis and remain persistent along the disease course. Specific basal, limbic, and occipital areas play a significant role in the pathogenesis of these alterations.
Have a Cake and Eat it Too: A Nanofluidic Hybrid Membrane with Both High Stability and Ionic Conductivity
Authors: Si Lianmeng , Xiao Hong , Xing Wensi , Song Rui , Li Zhaoqi , Li Yiju , Liang Xu , Song Jianwei , Shen Shengping 
Published: June 2024

AbstractTwo‐dimensional nanofluidic membranes are promising candidates for various applications, such as energy conversion and ionic sensing. However, simultaneously achieving high stability and high ion transport in a nanofluidic membrane remains a great challenge. Herein, a robust and durable aramid nanofiber/carboxylated aramid nanofiber (ANF/cANF) nanofluidic hybrid membrane is designed with high ion conductivity and selectivity via surface grafting engineering and hybridization strategies. Due to the inherent ordered and asymmetric molecular structure, the strong interchain interactions of the ANF and the strong interfacial interactions between the ANF and cANF enable the membrane to exhibit robust structural stability in a wet state. Meanwhile, the enhanced surface charge enabled by the surface functionalization of carboxyl groups on the ANF results in excellent ion transport. As a result, the conductivity of the membrane is 5 and 36 times higher than the ANF membrane and bulk solution, respectively. Importantly, the ionic conductivity and mechanical strength of the membrane remain unchanged even after immersing in water for 90 days, demonstrating favorable underwater application potential. Moreover, the membrane is recyclable and has superior processability, allowing for large‐scale processing. This work provides a new strategy for designing durable and high‐ion‐transporting nanofluidic membranes for ion sensing and energy conversion.
Rapid Self‐Healing of Robust Surface‐Tethered Covalent Adaptable Coatings
Authors: Capets Jacob A. , Yost Sierra F. , Vogt Bryan D. , Pester Christian W. 
Published: June 2024

AbstractThe incorporation of self‐healing properties to repair scratches (or other minor damage) has revolutionized the coating industry by increasing service life, sustainability, and optical appearance. This work addresses challenges with the robustness of self‐healing coatings through the inclusion of surface‐tethered covalently adaptable networks (CANs). Surface‐initiated polymerization is combined with spray‐coating to deposit polymers to produce coatings with reversible crosslinks to the tethered chains. These robust coatings are based on reversible vinylogous urethane bonds using 2‐(acetoacetoxy)ethyl methacrylate‐based polymers and tris(2‐aminoethyl) amine (TREN). Here, TREN enables reversible covalent bonding between the spray‐coated and surface‐tethered polymers. Without this polymer brush layer, the physisorbed CAN coatings fail to self‐heal completely, are labile to solvent, and exhibit shear delamination upon scratching. The utility of this tethered coating approach is highlighted through its ability to autonomously self‐heal incisions within seconds at elevated temperatures, or more steadily under ambient conditions. The key to these advancements is the use of polymer brushes as a primer layer to attach the CAN to enhance healing and improve the environmental robustness of the coating.
Mn(II)‐Aloe‐Emodin Nanoscale Coordination Polymer Enhances Ferroptosis by Synergistically Enhancing Reactive Oxygen Species Generation via the Nrf2–GPX4 Axis
Authors: Yang Xiaoxin , Li Chang , Ge Mengjun , Li Xiaoying , Zhao Wei , Guo Hu , Nie Hemin , Liu Jun 
Published: June 2024

AbstractFerroptosis induction is particularly promising for cancer therapy when the apoptosis pathway is compromised. Current strategies in nanomedicine for inducing ferroptosis primarily focus on promoting the accumulation of reactive oxygen species (ROS). However, the presence of intracellular antioxidants, such as nuclear factor erythroid 2‐related factor 2 (Nrf2), can limit the effectiveness of such therapy by activating detoxification systems and eliminating ROS. To overcome this challenge, we developed a synergistic ferroptosis‐inducing agent by modifying manganese (Mn2+)–1,8‐dihydroxy‐3‐hydroxymethyl‐anthraquinone (aloe‐emodin, AE) with polyvinyl pyrrolidone (PVP) to create nanoparticles (MAP NPs). In the tumor microenvironment, these NPs degraded and released AE and Mn(II), facilitating the generation of ROS and Mn(IV) through a Fenton‐like reaction between hydrogen peroxide (H2O2) and Mn(II). Mn(IV) subsequently interacts with glutathione (GSH) to induce a cyclic catalytic effect, and the depletion of GSH diminished the activation of glutathione‐dependent peroxidase 4 (GPX4). Furthermore, AE inhibits the activity of Nrf2 and depleted GSH, thereby synergistically enhancing antitumor efficacy. Here it is demonstrated that MAP NPs effectively generate a robust ROS storm within tumor cells, suggesting that high‐performance ferroptosis therapy is effective. Additionally, the inclusion of Mn(II) in the MAP NPs enables real‐time monitoring of therapeutic efficacy via magnetic resonance T1‐weighted contrast imaging.
A Cell Pre‐Wrapping Seeding Technique for Hydrogel‐Based Tubular Organ‐On‐A‐Chip
Authors: Nie Jing , Lou Sha , Pollet Andreas M. A. O. , van Vegchel Manon , Bouten Carlijn V. C. , den Toonder Jaap M. J. 
Published: June 2024

AbstractOrgan‐on‐a‐chip (OOC) models based on microfluidic technology are increasingly used to obtain mechanistic insight into (patho)physiological processes in humans, and they hold great promise for application in drug development and regenerative medicine. Despite significant progress in OOC development, several limitations of conventional microfluidic devices pose challenges. First, most microfluidic systems have rectangular cross sections and flat walls, and therefore tubular/ curved structures, like blood vessels and nephrons, are not well represented. Second, polymers used as base materials for microfluidic devices are much stiffer than in vivo extracellular matrix (ECM). Finally, in current cell seeding methods, challenges exist regarding precise control over cell seeding location, unreachable spaces due to flow resistances, and restricted dimensions/geometries. To address these limitations, an alternative cell seeding technique and a corresponding workflow is introduced to create circular cross‐sectioned tubular OOC models by pre‐wrapping cells around sacrificial fiber templates. As a proof of concept, a perfusable renal proximal tubule‐on‐a‐chip is demonstrated with a diameter as small as 50 µm, cellular tubular structures with branches and curvature, and a preliminary vascular‐renal tubule interaction model. The cell pre‐wrapping seeding technique promises to enable the construction of diverse physiological/pathological models, providing tubular OOC systems for mechanistic investigations and drug development.
Lefamulin Overcomes Acquired Drug Resistance via Regulating Mitochondrial Homeostasis by Targeting ILF3 in Hepatocellular Carcinoma
Authors: Zheng Ying , Ye Shengtao , Huang Shiyu , Cheng Yang , Zhang Yanqiu , Leng Yingrong , He Mengmeng , Wu Enyi , Chen Junxin , Kong Lingyi , Zhang Hao 
Published: June 2024

AbstractAcquired resistance represents a critical clinical challenge to molecular targeted therapies such as tyrosine kinase inhibitors (TKIs) treatment in hepatocellular carcinoma (HCC). Therefore, it is urgent to explore new mechanisms and therapeutics that can overcome or delay resistance. Here, a US Food and Drug Administration (FDA)‐approved pleuromutilin antibiotic is identified that overcomes sorafenib resistance in HCC cell lines, cell line‐derived xenograft (CDX) and hydrodynamic injection mouse models. It is demonstrated that lefamulin targets interleukin enhancer‐binding factor 3 (ILF3) to increase the sorafenib susceptibility of HCC via impairing mitochondrial function. Mechanistically, lefamulin directly binds to the Alanine‐99 site of ILF3 protein and interferes with acetyltransferase general control non‐depressible 5 (GCN5) and CREB binding protein (CBP) mediated acetylation of Lysine‐100 site, which disrupts the ILF3‐mediated transcription of mitochondrial ribosomal protein L12 (MRPL12) and subsequent mitochondrial biogenesis. Clinical data further confirm that high ILF3 or MRPL12 expression is associated with poor survival and targeted therapy efficacy in HCC. Conclusively, this findings suggest that ILF3 is a potential therapeutic target for overcoming resistance to TKIs, and lefamulin may be a novel combination therapy strategy for HCC treatment with sorafenib and regorafenib.
Zwitterion‐Lubricated Hydrogel Microspheres Encapsulated with Metformin Ameliorate Age‐Associated Osteoarthritis
Authors: Hou Jiahui , Lin Yanpeng , Zhu Chencheng , Chen Yupeng , Lin Rongmin , Lin Hancheng , Liu Dahai , Guan Daogang , Yu Bin , Wang Jun , Wu Hangtian , Cui Zhuang 
Published: June 2024

AbstractChondrocyte senescence and reduced lubrication play pivotal roles in the pathogenesis of age‐related osteoarthritis (OA). In the present study, highly lubricated and drug‐loaded hydrogel microspheres are designed and fabricated through the radical polymerization of sulfobetaine (SB)‐modified hyaluronic acid methacrylate using microfluidic technology. The copolymer contains a large number of SB and carboxyl groups that can provide a high degree of lubrication through hydration and form electrostatic loading interactions with metformin (Met@SBHA), producing a high drug load for anti‐chondrocyte senescence. Mechanical, tribological, and drug release analyses demonstrated enhanced lubricative properties and prolonged drug dissemination of the Met@SBHA microspheres. RNA sequencing (RNA‐seq) analysis, network pharmacology, and in vitro assays revealed the extraordinary capacity of Met@SBHA to combat chondrocyte senescence. Additionally, inducible nitric oxide synthase (iNOS) has been identified as a promising protein modulated by Met in senescent chondrocytes, thereby exerting a significant influence on the iNOS/ONOO‐/P53 pathway. Notably, the intra‐articular administration of Met@SBHA in aged mice ameliorated cartilage senescence and OA pathogenesis. Based on the findings of this study, Met@SBHA emerges as an innovative and promising strategy in tackling age‐related OA serving the dual function of enhancing joint lubrication and mitigating cartilage senescence.
Emerging 2D Nanomaterials‐Integrated Hydrogels: Advancements in Designing Theragenerative Materials for Bone Regeneration and Disease Therapy
Authors: Zorrón Melanie , Cabrera Agustín López , Sharma Riya , Radhakrishnan Janani , Abbaszadeh Samin , Shahbazi Mohammad‐Ali , Tafreshi Omid Aghababaei , Karamikamkar Solmaz , Maleki Hajar 
Published: June 2024

AbstractThis review highlights recent advancements in the synthesis, processing, properties, and applications of 2D‐material integrated hydrogels, with a focus on their performance in bone‐related applications. Various synthesis methods and types of 2D nanomaterials, including graphene, graphene oxide, transition metal dichalcogenides, black phosphorus, and MXene are discussed, along with strategies for their incorporation into hydrogel matrices. These composite hydrogels exhibit tunable mechanical properties, high surface area, strong near‐infrared (NIR) photon absorption and controlled release capabilities, making them suitable for a range of regeneration and therapeutic applications. In cancer therapy, 2D‐material‐based hydrogels show promise for photothermal and photodynamic therapies, and drug delivery (chemotherapy). The photothermal properties of these materials enable selective tumor ablation upon NIR irradiation, while their high drug‐loading capacity facilitates targeted and controlled release of chemotherapeutic agents. Additionally, 2D‐materials ‐infused hydrogels exhibit potent antibacterial activity, making them effective against multidrug‐resistant infections and disruption of biofilm generated on implant surface. Moreover, their synergistic therapy approach combines multiple treatment modalities such as photothermal, chemo, and immunotherapy to enhance therapeutic outcomes. In bio‐imaging, these materials serve as versatile contrast agents and imaging probes, enabling their real‐time monitoring during tumor imaging. Furthermore, in bone regeneration, most 2D‐materials incorporated hydrogels promote osteogenesis and tissue regeneration, offering potential solutions for bone defects repair. Overall, the integration of 2D materials into hydrogels presents a promising platform for developing multifunctional theragenerative biomaterials.
Targeting Hydrogel for Intelligent Recognition and Spatiotemporal Control in Cell‐Based Therapeutics
Authors: Hou Weilin , Mao Wei , Sun Jun , Liu Zhiqiang , Shen Wei , Lee Hian Kee , Tang Sheng 
Published: June 2024

AbstractSmart drug platforms based on spatiotemporally controlled release and integration of tumor imaging are expected to overcome the inefficiency and uncertainty of traditional theranostic modes. In this study, a composite consisting of a thermosensitive hydrogel (polyvinyl alcohol‐carboxylic acid hydrogel (PCF)) and a multifunctional nanoparticle (Fe3O4@Au/Mn(Zn)‐4‐carboxyphenyl porphyrin/polydopamine (FAMxP)) is developed to combine tumor immunogenic cell death (ICD)/immune checkpoint blockade (ICB) therapy under the guidance of magnetic resonance imaging (MRI) and fluorescence imaging (FI). It can not only further recognize the target cells through the folate receptor of tumor cells, but also produce thermal dissolution after exposure to near‐infrared light to slowly release FAMxP in situ, thereby prolonging the treatment time and avoiding tumor recurrence. As FAMxP entered the tumor cells, it released FAMx in a pH‐dependent manner. Chemodynamic, photothermal and photodynamic therapy can cause significant ICD in cancer cells. ICB can thus be further enhanced by injecting anti‐programmed cell death ligand 1, improving the effectiveness of tumor treatment. The developed PCF‐FAMxP composite hydrogel may represent an updated drug design approach with simple compositions for cooperative MRI/FI‐guided targeted therapeutic pathways for tumors.
Blue Luminescent Boron Complexes Based on N,N‐Type Imidazo[1,5‐a]pyridine Ligand for Mitochondrial Imaging
Authors: Yagishita Fumitoshi , Katayama Tetsuro , Kawamura Yuta , Watanabe Guran , Abe Sota , Ogawa Itsuki , Tabata Atsushi , Yoshida Yasushi , Masu Hyuma , Ueta Shoko , Arakawa Yukihiro , Minagawa Keiji , Furube Akihiro , Imada Yasushi 
Published: June 2024

AbstractWe have synthesized two boron complexes based on N,N‐type bidentate imidazo[1,5‐a]pyridine ligands exhibiting blue emission with good quantum yields in solution. The transient absorption and theoretical studies of the boron complexes indicated the intramolecular charge transfer nature. In addition, one specific complex, 1 a was found to work as a mitochondrial imaging agent.
Electrochemical Synthesis of Isoquinoline Sulfone Compounds and Study of their Inhibition of Acetolactate Synthase Enzyme Activity
Authors: Peng Zhixiong , Huang Jiayu , Chen Yao , Liu Ke , Xiong Yunkui 
Published: June 2024

AbstractBoth of sulfone compounds and isoqunoline derivatives are important compounds. In this paper, Isoquinoline C1 and C3 Sulfone compounds have been synthesized by electrochemical means using isoquinoline N‐Oxides and some sodium benzenesulfinate as reactants, inexpensive and easily available sodium iodide as electrolytes, and a mixture of acetonitrile, water and acetic acid as reaction solvents. By using the method, a series of isoquinoline sulfone compounds have been synthesized in high yields. The reaction does not need oxidants and catalysts, and the reaction conditions are mild, environmentally friendly, efficient and suitable. In addition, we also carried out ALS enzyme inhibition experiments on the synthesized isoquinoline sulfone compounds, six of the isoquinoline sulfone compounds synthesized herein were found to inhibit ALS enzyme at a high rate, four of them inhibited ALS enzyme at a rate comparable to that of the commercialized herbicides Halosulfuron and Broadstrike, and the other two compounds also inhibited ALS enzyme at a rate higher than that of Halosulfuron and Broadstrike. Therefore, it is possible to find lead compounds with good herbicidal activity potential from these 6 compounds.