bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a machine learning-based risk prediction model for depression in middle-aged and elderly hypertensive people in China: a longitudinal study
Authors: Ai Fangzhu , Li Enguang , Ji Qiqi , Zhang Huijun 
Published: May 2024

BackgroundHypertension is a common chronic disease that can trigger symptoms such as anxiety and depression. Therefore, it is essential to predict their risk of depression. The aim of this study is to find the best prediction model and provide effective intervention strategies for health professionals.MethodsThe study subjects were 2733 middle-aged and older adults who participated in the China Health and Retirement Longitudinal Study (CHARLS) between 2018 and 2020. R software was used for Lasso regression analysis to screen the best predictor variables, and logistic regression, random forest and XGBoost models were constructed. Finally, the prediction efficiency of the three models was compared.ResultsIn this study, 18 variables were included, and LASSO regression screened out 10 variables that were important for the establishment of the model. Among the three models, Logistic Regression model showed the best performance in various evaluation indicators.ConclusionThe prediction model based on machine learning can accurately assess the likelihood of depression in middle-aged and elderly patients with hypertension in the next three years. And by combining Logistic regression and nomograms, we were able to provide a clear interpretation of personalized risk predictions.
Frailty and poor physical functioning as risk factors for driving cessation
Authors: Mielenz Thelma J. , Jia Haomiao , DiGuiseppi Carolyn G. , Strogatz David , Andrews Howard F. , Molnar Lisa J. , Eby David W. , Hill Linda L. , Li Guohua 
Published: May 2024

IntroductionFrailty and low physical performance are modifiable factors and, therefore, targets for interventions aimed at delaying driving cessation (DC). The objective was to determine the impact of frailty and physical performance on DC.MethodsMultisite prospective cohort of older drivers. The key inclusion criteria are as follows: active driver age 65–79 years, possessing a valid driver’s license, without significant cognitive impairment, and driving a 1996 car or a newer model car. Of the 2,990 enrolled participants, 2,986 (99.9%) had at least one frailty or Short Physical Performance Battery (SPPB) measure and were included in this study. In total, 42% of participants were aged 65–69 years, 86% were non-Hispanic white, 53% were female, 63% were married, and 41% had a high degree of education. The Fried Frailty Phenotype and the Expanded Short Physical Performance Battery (SPPB) from the National Health and Aging Trends Study were utilized. At each annual visit, DC was assessed by the participant notifying the study team or self-reporting after no driving activity for at least 30 days, verified via GPS. Cox proportional hazard models, including time-varying covariates, were used to examine the impact of the SPPB and frailty scores on time to DC. This assessment included examining interactions by sex.ResultsSeventy-three participants (2.4%) stopped driving by the end of year 5. Among women with a fair SPPB score, the adjusted hazard ratio (HR) of DC was 0.26 (95% confidence interval (CI) 0.10–0.65) compared to those with a poor SPPB score. For those with a good SPPB score, the adjusted HR of DC had a p-value of &lt;0.001. Among men with a fair SPPB score, the adjusted hazard ratio (HR) of DC was 0.45 (95% CI 0.25–0.81) compared to those with a poor SPPB score. For men with a good SPPB score, the adjusted HR of DC was 0.19 (95% CI 0.10–0.36). Sex was not an effect modifier between frailty and DC. For those who were categorized into pre-frail or frail, the adjusted ratio of HR to DC was 6.1 (95% CI 2.7–13.8) compared to those who were not frail.Conclusion and relevanceFrailty and poor physical functioning are major risk factors for driving cessation. Staying physically active may help older adults to extend their driving life expectancy and mobility.
Current and future development of thermal grids in Switzerland: an organizational perspective
Authors: Speich Matthias , Chambers Jonathan , Ulli-Beer Silvia 
Published: May 2024

IntroductionThermal grids are key to decarbonizing heating and cooling. However, their development is a complex socio-technical process. This study aims to (1) understand the thermal grid ecosystem’s development under changing political, economic and cultural frame conditions, (2) elicit actors’ perception of options for future development, and (3) identify concrete problem situations for the future development of thermal grids and describe how to address them.MethodsWe draw on 18 semi-structured interviews with decision-makers in utilities, municipal administrations, planning and engineering companies and intermediary organizations.ResultsWith increased strategic importance of thermal grids, the ecosystem has been in a phase of rapid growth and professionalization in the last 10–15 years. This is reflected in a rapid increase in the number of grids, but also in capability and capacity building and greater quality standards. This transformation has been supported by a resource pool (e.g., knowledge base, capacities and capabilities, networks or decision-support tools), built partly through the value network’s activities and partly through purposeful orchestration by associations, authorities, utilities and other actors. While policy developments, energy price fluctuations and changes in customer perceptions spurred demand, thermal grid development is limited by supply-side constraints, especially a skill shortage. Four interrelated problem situations were identified: (1) Municipalities lack the resources to manage the implementation of their energy strategies. (2) The modernization of thermal grids and integration of low-carbon heat sources entails high transaction costs. (3) The implementation of thermal grids is subject to delays and quality risks, with consequences for the timely achievement of decarbonization goals. (4) Customers face uncertainty on available options, as well as on the systemic impacts of their decisions.DiscussionBased on current practices, suggestions by the interviewees, as well as a comparison with the international literature, we identify four development areas to address these problem situations: (1) Empower and support municipalities. (2) Manage the scarcity of qualified workforce. (3) Leverage digital technology for the planning, realization and operation of thermal grids. (4) Pilot technological and organizational innovations together. This complements the set of previously suggested measures to support the development of thermal grids, which focused strongly on technical and regulatory aspects.
Chlorogenic acid attenuates tet (X)-mediated doxycycline resistance of Riemerella anatipestifer
Authors: Han Yuwen , Li Min , Su Dehai , Xiong Shiyu , Feng Youshu , Deng Qin , Ding Huanzhong 
Published: May 2024

IntroductionThe increasing resistance of R. anatipestifer has posed a significant threat to the poultry industry in recent years. The tet gene is the primary determinant of tetracycline resistance in numerous bacteria, and the enzyme modification gene tet(X) is predominantly detected in tetracycline-resistant R. anatipestifer strains.MethodsIn this study, we evaluated the susceptibility of both the standard strain and clinical isolates of R. anatipestifer to doxycycline. And the expression levels of tet(X), tet(A), and tet(O) genes were detected. To assess drug susceptibility, shuttle plasmids were constructed to transfer the tet(X) gene into the standard strain of R. anatipestifer followed by treatment with chlorogenic acid.Results and discussionThe results revealed that the minimum inhibitory concentration of doxycycline for the standard strain was 0.25μg/mL, whereas it exceeded 8μg/mL for the clinical isolates. Furthermore, there was a significant upregulation observed in expression levels of tet(X), tet(A), and tet(O) genes among induced strains. Interestingly, when transferring the tet(X) gene into the standard strain, its sensitivity to doxycycline decreased; however, MIC values for chlorogenic acid remained consistent between both standard and drug-resistant strains of R. anatipestifer. Moreover, we made a surprising discovery that screening passage with chlorogenic acid resulted in increased sensitivity of R. anatipestifer to doxycycline. Further analysis demonstrated a reversal in expression trends among three differentially expressed genes within induced drug resistance group after intervention with chlorogenic acid. The main objective behind this study is to investigate both killing effect exerted by chlorogenic acid on drug-resistant R. anatipestifer as well as its regulatory impact on drug resistance genes. This will provide novel insights and theoretical basis towards development of chlorogenic acid as a promising drug for treatment and control of drug resistance in R. anatipestifer.
Perkembangan Teknologi Pakan Ikan Otomatis dalam Perikanan Modern : Tinjauan Literatur
Authors: Putri Diana Eka 
Published: May 2024

Perkembangan teknologi di era revolusi industri 4.0 saat ini sudah semakin canggih, dengan kemajuannya yang sangat pesat ini memudahkan manusia dalam mengerjakan pekerjaannya. Salah satu kemajuan dari teknologi yang ada saat ini yaitu kemampuannya untuk menjalankan suatu alat secara otomatis. Otomatisasi juga berperan penting dalam bidang budidaya ikan, dimana para pelaku usaha ditantang untuk berinovasi dalam menghadapi revolusi industri. Konsep dari budidaya ikan modern sendiri melibatkan pemberian pakan otomatis berdasarkan waktu yang sudah ditentukan sebelumnya. Hal ini memungkinkan pemilik budidaya ikan memberi pakan secara teratur dan konsisten. Metode penelitian yang digunakan adalah systematic literature review, dengan mengumpulkan data dari database Google Scholar. Dengan menggunakan kata kunci (“Pakan Ikan Otomatis” DAN “IoT”) didapatkan sebanyak 402 artikel kemudian di seleksi hingga mendapatkan 20 artikel yang relevan dengan topik yang diambil untuk dianalisis. Studi literatur ini memberikan pemahaman mendalam tentang tren dan tantangan dalam pengembangan alat pakan ikan otomatis berbasis IoT. Hail artikel ini memberikan gambaran perkembangan pakan ikan otomatis. Dapat disimpulkan bahwa pakan ikan otomatis menggunakan salah satu mikrokontroller seperti Arduino Uno atau Arduino Nano, NodeMCU ESP8266, dan Wemos D1R1 atau Wemos D1 mini. Secara keseluruhan, para peneliti telah membuat pakan ikan otomatis dengan akurasi waktu yang sesuai dengan yang sudah ditetapkan sebelumnya.
Biossensores: avanços e desafios no diagnóstico precoce do câncer de boca
Authors: Ferreira Humberto Freitas Silveira , Muniz Luciana Vieira 
Published: May 2024

O Carcinoma Espinocelular Oral (CEC) é o câncer mais comum da cabeça e pescoço. No Brasil, em 2020, ocorreram 6.192 óbitos por câncer da cavidade oral (C00 -C10), correspondendo a um risco de morte de 2,92 por 100 mil habitantes. Entre os homens, foram 4.767 óbitos (4,60 por 100 mil) e, em mulheres, 1.425 (1,32 por 100 mil). Os cânceres orais ocupam a oitava colocação dentre os cânceres mais frequentes e apresentam uma elevada taxa de mortalidade. O carcinoma de células escamosas oral representa mais de 90% de todos os tipos histológicos. Apesar dos avanços em nosso conhecimento das causas e fatores de risco associados ao CEC, as taxas de sobrevivência para câncer oral, não melhoraram substancialmente nos últimos quarenta anos. Sua alta mortalidade é atribuída ao fato de a doença ser muitas vezes diagnosticada tardiamente. Uma pesquisa realizada em Feira de Santana – BA no ano de 2006 relatou que o nível de confiança para realizar procedimentos de diagnóstico de câncer bucal foi considerado baixo para 69,5% dos profissionais. Para Neville a capacidade de controlar o câncer oral e orofaríngeo dependerá de dois pilares: prevenção e diagnóstico precoce. Neste contexto, estudos com biomarcadores e biossensores têm mostrado um campo promissor para as pesquisas no campo da Oncologia. O fluído salivar passou a ser utilizado como meio de diagnóstico para doenças sistêmicas e crônicas como o caso de neoplasias, tendo como exemplo o câncer de cavidade oral. Os biossensores podem avaliar com precisão e assertividade o número de biomarcadores, auxiliando no diagnóstico correto do desenvolvimento de CEC. Esta biotecnologia pode ser útil para triagem de pacientes de alto risco. O diagnóstico precoce do CEC é essencial para o sucesso tratamento da doença e melhora dos parâmetros de qualidade de vida e os estudos com novas tecnologias têm permitido a individualização de alguns tratamentos e o desenvolvimento da medicina personalizada e humanizada.
Aspectos clínicos e moleculares do Alzheimer – revisão de literatura
Authors: Mota Williams Queiroz , Lobão Italo da Silva , Cunha João Victor Lopes da , Ferreira Lucas Aires Alencar , Ramos Gabriel Lima , Silva Shelda Marlice Fernandes da , Sousa Ian Silva de , Moreira Higo Nasser Santanna 
Published: May 2024

A doença de Alzheimer (DA) é um distúrbio neurodegenerativo multifatorial progressivo caracterizado pelo acúmulo de agregados anormais de proteínas no cérebro, como placas de amiloide e emaranhados de tau, o que leva ao declínio gradual das habilidades cognitivas e, eventualmente, prejudica o funcionamento diário da função mental. O objetivo desse estudo foi descrever de forma ampla os aspectos clínicos e moleculares da doença de Alzheimer para os profissionais da saúde. As bases de dados que foram utilizadas nessa revisão incluem Scientific Electronic Library Online (SciELO) e National Library of Medicine (MedLine/PubMed). Dentre os artigos científicos que foram consultados, estes compreendem àqueles publicados nos últimos dez anos. Os trabalhos aqui discutidos, de maneira geral, abordam sobre a importância molecular e clínica da DA, tendo em vista que ela é complexa e multifatorial. Em suma, o processo de desenvolvimento científico-teórico sobre a DA não pode estagnar, e é de extrema importância que pesquisas nesse tema sejam publicadas, visando, assim, melhorar o processo de interpretação e compreensão da fisiopatologia da DA.
Tumor desmóide mesentérico em paciente com antecedente de cirurgia bariátrica videolaparoscópica: um relato de caso
Authors: Matos Mateus Santos , Paixão Filho Adriano Silvério da , Sant’Anna Carolina Vasconcellos , Godinho Stella Custódio , Sabbag Anwar Fausto Felix , Carvalho Sérgio , Dib Jorge Adas , Ricci Bruno Vita 
Published: May 2024

Os Tumores desmoides são lesões benignas de progressão local invasiva, isto acarreta em sintomas variados conforme sua localização. O tratamento dessas lesões que é em grande parte dos casos cirúrgicos, ainda com grande importância como forma de tratamento, vem progredindo rapidamente com novas opções terapêuticas com boa evolução clínica e menor morbidade, este artigo discute os tipos de tratamento para essas neoplasias.
Knowledge-based: facilitating access to medicines in Latin America
Authors: Ferreira Talita da Silva , Pauletti Giovanni M , Fernandes Eduardo Agostinho Freitas , Campos Andrés Figueroa , Aceituno Alexis , Cabrera-Pérez Miguel Ángel , Triana Diego Gutierrez , Vázquez-Suárez Luis 
Published: May 2024

The World Health Organization (WHO), with the scientific support of the International Pharmaceutical Federation (FIP), guides the development of multisource pharmaceutical products for market authorization using in vivo bioequivalence studies or, where applicable, in vitro biowaiver strategies based on the Biopharmaceutical Classification System (BCS). A review of the regulatory framework guiding generic medicines approval in Latin American countries revealed that less than 50% of regional health authorities offer a generic medicines development pathway utilizing a BCS-based biowaiver strategy. Aligned with the ONE FIP Strategy to facilitate access to medicines, a regional case study was carried out to implement and harmonize BCS-based biowaiver knowledge in Latin American countries. A steering committee involving regional representatives from health authorities, the pharmaceutical industry, and universities were established to coordinate to develop activities. A series of digital engagement events were held in Spanish and English with representatives from Latin America to share knowledge on BCS-based regulatory strategy, promote collaborations, and explore the alignment of biowaiver approval and regulatory pathways among Latin American countries. Feedback from diverse Latin American stakeholders demonstrated inconsistent implementation of bioequivalence testing within the region. However, there is support for a synergistic approach among countries to reduce duplication and increase efficiency in market authorization for generic medicines. This includes alignment with the WHO Prequalification of Medicines program as well as the development of a computational database for the classification of active pharmaceutical ingredients to demonstrate therapeutic interchangeability of immediate-release oral dosage forms according to the BCS. FIP-facilitated digital learning opportunities raised awareness of the BCS-based biowaiver regulatory strategy among Latin American stakeholders. It resulted in a plan to continually strengthen collaborative efforts in the region to harmonize regulations relevant to drug development generics medicines to introduce cost-effective medicines products that benefit public health.
Análise epidemiológica das internações por queimaduras em Alagoas (2020-2023)
Authors: Andrade Pedro Nogueira de , Ribeiro Maria Victoria de Morais Born , Almeida Lucas Fernandes de Carvalho , Alves Darah Yasmim Moreira , Fortes Cecile Hora Figueiredo , Melo Guilherme Felix Barbosa de , Martins Luis Mateus Camelo França , Pessoa Ana Carolina Feitosa , Badaró Leandro Rodrigo Caetano Neves Valadares , Maranhão Jéssica Mahine Rocha , Araújo Maria Luísa Granja de , Amorim Carolina Melo de , Santos Pedro Rafael Araujo Ramos dos , Lima Alessandro Araújo de , Silva Mayra Alencar da 
Published: May 2024

Introdução: As queimaduras representam um desafio considerável para a saúde pública, destacando-se como uma das principais causas de morte entre crianças no Brasil. No entanto, a falta de dados e informações disponíveis dificulta a implementação de medidas preventivas. Objetivo: Investigar a prevalência das internações por queimaduras no Estado de Alagoas, no período de 2020 a 2023. Metodologia: Estudo epidemiológico descritivo retrospectivo com dados extraídos na base de dados do TABNET/DATASUS. As variáveis de interesse foram: ano de notificação, região de notificação, faixa etária, cor/raça. Resultados: Em 2023, registrou-se a maior incidência de queimaduras, atingindo 33,69%. O estudo revelou que a faixa etária mais afetada foi a de 1 a 4 anos, representando 29,30% do total. Quanto aos tipos de atendimento, predominou a modalidade de urgência, com 1.137 casos, correspondendo a 68,28% do total. Conclusão: Reconhecer os aspectos clínicos e epidemiológicos é crucial para desenvolver medidas de cuidado e assistência comunitária. É fundamental alertar a sociedade e as autoridades sobre a necessidade de investir em estratégias de prevenção e segurança.
Multiscale Control of Nanofiber-Composite Hydrogel for Complex 3D Cell Culture by Extracellular Matrix Composition and Nanofiber Alignment
Authors: Choi Cholong , Yun Eunhye , Song Minju , Kim Jiyun , Son Jae Sung , Cha Chaenyung 
Published: May 2024

In order to manipulate the complex behavior of cells in a 3-dimensional (3D) environment, it is important to provide the microenvironment that can accurately portray the complexity of highly anisotropic tissue structures. However, it is technically challenging to generate a complex microenvironment using conventional biomaterials that are mostly isotropic with limited bioactivity. In this study, the gelatin-hyaluronic acid hydrogel incorporated with aqueous-dispersible, short nanofibers capable of in situ alignment is developed to emulate the native heterogeneous extracellular matrix consisting of fibrous and non-fibrous components. The gelatin nanofibers containing magnetic nanoparticles, which could be aligned by external magnetic field, are dispersed and embedded in gelatin-hyaluronic acid hydrogel encapsulated with dermal fibroblasts. The aligned nanofibers via magnetic field could be safely integrated into the hydrogel, and the process could be repeated to generate larger 3D hydrogels with variable nanofiber alignments. The aligned nanofibers in the hydrogel can more effectively guide the anisotropic morphology (e.g., elongation) of dermal fibroblasts than random nanofibers, whereas myofibroblastic differentiation is more prominent in random nanofibers. At a given nanofiber configuration, the hydrogel composition having intermediate hyaluronic acid content induces myofibroblastic differentiation. These results indicate that modulating the degree of nanofiber alignment and the hyaluronic acid content of the hydrogel are crucial factors that critically influence the fibroblast phenotypes. The nanofiber-composite hydrogel capable of directional nanofiber alignment and tunable material composition can effectively induce a wide array of phenotypic plasticity in 3D cell culture.
Avaliação da produtividade do milho por seis anos subsequentes após a aplicação de biocarvão em um latossolo amarelo no Cerrado
Authors: Santana Douglas Martins de , Dalto Paulo Henrique 
Published: May 2024

O biocarvão é um produto estável, formado pela decomposição de materiais vegetais em temperaturas baixas e com pequena concentração de oxigênio. Sua incorporação proporciona melhorias nos atributos do solo e nas produtividades das culturas, porém a avaliação desses efeitos a longo prazo é escassa. Objetivou-se analisar o efeito do biocarvão na produtividade do milho durante seis anos após a aplicação do produto em um Latossolo Amarelo Distrófico. Os ensaios foram conduzidos na área experimental do Instituto Federal do Piauí – Campus Uruçuí, com delineamento em blocos casualizados em esquema de parcelas subdividas 6 x 6, com três repetições. As parcelas foram compostas pelas doses de biocarvão: 0,0 (testemunha); 1,0; 2,0; 4,0; 8,0 e 16 Mg ha-1; e as subparcelas pelas safras: 2015/16; 2016/17; 2017/18; 2018/19; 2019/20 e 2020/21. O biocarvão foi originado de madeira de mata nativa da região e a cultura do milho CV. 30F53 foi utilizada como indicador em todas as safras. Cada subparcela possuiu uma área útil de 12 m2, sendo colhidas apenas as 3 linhas centrais, manualmente. Houve interação significativa entre as doses de biocarvão e as safras para a produtividade do milho, indicando que quanto maiores as doses aplicadas, maior a produtividade. Nos anos com baixos índices pluviométricos, áreas com e sem adição de biocarvão não apresentaram diferenças estatísticas, evidenciando que a falta da água foi o principal limitador da produtividade. A sexta safra estudada ainda mostrou benefícios da aplicação de biocarvão, indicando que este produto proporciona benefícios a longo prazo para a cultura do milho.