bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial transfer from Adipose stem cells to breast cancer cells drives multi-drug resistance
Authors: Del Vecchio Vitale , Rehman Ayesha , Panda Sameer Kumar , Torsiello Martina , Marigliano Martina , Nicoletti Maria Maddalena , Ferraro Giuseppe Andrea , De Falco Vincenzo , Lappano Rosamaria , Lieto Eva , Pagliuca Francesca , Caputo Carlo , La Noce Marcella , Papaccio Gianpaolo , Tirino Virginia , Robinson Nirmal , Desiderio Vincenzo , Papaccio Federica 
Published: June 2024

Abstract
Background
Breast cancer (BC) is a complex disease, showing heterogeneity in the genetic background, molecular subtype, and treatment algorithm. Historically, treatment strategies have been directed towards cancer cells, but these are not the unique components of the tumor bulk, where a key role is played by the tumor microenvironment (TME), whose better understanding could be crucial to obtain better outcomes.

Methods
We evaluated mitochondrial transfer (MT) by co-culturing Adipose stem cells with different Breast cancer cells (BCCs), through MitoTracker assay, Mitoception, confocal and immunofluorescence analyses. MT inhibitors were used to confirm the MT by Tunneling Nano Tubes (TNTs). MT effect on multi-drug resistance (MDR) was assessed using Doxorubicin assay and ABC transporter evaluation. In addition, ATP production was measured by Oxygen Consumption rates (OCR) and Immunoblot analysis.

Results
We found that MT occurs via Tunneling Nano Tubes (TNTs) and can be blocked by actin polymerization inhibitors. Furthermore, in hybrid co-cultures between ASCs and patient-derived organoids we found a massive MT. Breast Cancer cells (BCCs) with ASCs derived mitochondria (ADM) showed a reduced HIF-1α expression in hypoxic conditions, with an increased ATP production driving ABC transporters-mediated multi-drug resistance (MDR), linked to oxidative phosphorylation metabolism rewiring.

Conclusions
We provide a proof-of-concept of the occurrence of Mitochondrial Transfer (MT) from Adipose Stem Cells (ASCs) to BC models. Blocking MT from ASCs to BCCs could be a new effective therapeutic strategy for BC treatment.
Statistical analysis plan for the TRANSLATE (TRANSrectal biopsy versus Local Anaesthetic Transperineal biopsy Evaluation of potentially clinically significant prostate cancer) multicentre randomised controlled trial
Authors: Marian Ioana R. , Ooms Alexander , Holmes Jane , Parkes Matthew J. , Lamb Alastair D. , Bryant Richard J. 
Published: June 2024

Abstract
Background
The TRANSLATE (TRANSrectal biopsy versus Local Anaesthetic Transperineal biopsy Evaluation) trial assesses the clinical and cost-effectiveness of two biopsy procedures in terms of detection of clinically significant prostate cancer (PCa). This article describes the statistical analysis plan (SAP) for the TRANSLATE randomised controlled trial (RCT).

Methods/design
TRANSLATE is a parallel, superiority, multicentre RCT. Biopsy-naïve men aged ≥ 18 years requiring a prostate biopsy for suspicion of possible PCa are randomised (computer-generated 1:1 allocation ratio) to one of two biopsy procedures: transrectal (TRUS) or local anaesthetic transperineal (LATP) biopsy. The primary outcome is the difference in detection rates of clinically significant PCa (defined as Gleason Grade Group ≥ 2, i.e. any Gleason pattern ≥ 4 disease) between the two biopsy procedures. Secondary outcome measures are th eProBE questionnaire (Perception Part and General Symptoms) and International Index of Erectile Function (IIEF, Domain A) scores, International Prostate Symptom Score (IPSS) values, EQ-5D-5L scores, resource use, infection rates, complications, and serious adverse events. We describe in detail the sample size calculation, statistical models used for the analysis, handling of missing data, and planned sensitivity and subgroup analyses. This SAP was pre-specified, written and submitted without prior knowledge of the trial results.

Discussion
Publication of the TRANSLATE trial SAP aims to increase the transparency of the data analysis and reduce the risk of outcome reporting bias. Any deviations from the current SAP will be described and justified in the final study report and results publication.

Trial registration
International Standard Randomised Controlled Trial Number ISRCTN98159689, registered on 28 January 2021 and registered on the ClinicalTrials.gov (NCT05179694) trials registry.
Effectiveness of some plant extracts in biocontrol of induced onion basal rot disease in greenhouse conditions
Authors: Hegazy Mohamed G. A. , Ahmed Abdel-Raddy M. , Yousef Ahmed Fathy , Ali Waleed M. , Nasr Alyaa , Elshazly Ezzat H. , Shalaby Mohamed E. , Teiba Islam I. , Al-Bedak Osama A. M. 
Published: June 2024

AbstractOne of Egypt’s most notable and historically significant vegetable crops is the Liliaceae plant, Allium cepa L. In this study, the effectiveness of methanolic extracts of Artemisia absinthium leaves, Calotropis procera latex, Moringa oleifera seeds, and Syzygium aromaticum clove was investigated in vitro and, in a greenhouse, setting against Fusarium oxysporum, the pathogen that causes onion basal rot in Assiut Governorate, Egypt. The S. aromaticum extract exhibited the inhibition peak (63.3%), whereas the A. absinthium extract had the lowest inhibition impact against F. oxysporum growth (41.1%). The gas chromatography-mass spectroscopy (GC–MS) analysis revealed that 82 important compounds, with abundances ranging from low to high, were present in the tested S. aromaticum’s methanolic extract. The primary components were acetaldehyde, hydroxy- and 2-propanone, 1,1,3,3-tetrachloro-(42.71%), 1,2-ethanediol, and methyl alcohol (34.01%). In comparison to the infected control, the disease severity was significantly reduced by 20% with the use of a plant extracts mixture and Dovex 50% and increased by 62.22% with the use of an extract from A. absinthium. When compared to the infected control, onion plant fresh weight and dry weight were considerably higher under the clove extract therapy. The plant extracts used in this study’s testing contain a number of active ingredients, including amino acids, vitamins, minerals, antioxidants, and enzymes, which is probably why they have such positive impacts. The application of a combination of plant extracts was suggested as a feasible strategy for improving the growth and productivity of onion plants by the study’s findings. More research is needed to comprehend the mechanisms by which plant extracts promote plant development and to optimize the concentration and timing of administration.
ALKBH5-mediated m6A demethylation of Runx2 mRNA promotes extracellular matrix degradation and intervertebral disc degeneration
Authors: Lei Yu , Zhan Enyu , Chen Chao , Hu Yaoquan , Lv Zhengpin , He Qicong , Wang Xuenan , Li Xingguo , Zhang Fan 
Published: June 2024

Abstract
Background
N6-methyladenosine (m6A) methylation is a prevalent RNA modification implicated in various diseases. However, its role in intervertebral disc degeneration (IDD), a common cause of low back pain, remains unclear.

Results
In this investigation, we explored the involvement of m6A demethylation in the pathogenesis of IDD. Our findings revealed that ALKBH5 (alkylated DNA repair protein AlkB homolog 5), an m6A demethylase, exhibited upregulation in degenerative discs upon mild inflammatory stimulation. ALKBH5 facilitated m6A demethylation within the three prime untranslated region (3′-UTR) of Runx2 mRNA, consequently enhancing its mRNA stability in a YTHDF1 (YTH N6-methyladenosine RNA binding protein F1)-dependent manner. The subsequent elevation in Runx2 expression instigated the upregulation of ADAMTSs and MMPs, pivotal proteases implicated in extracellular matrix (ECM) degradation and IDD progression. In murine models, subcutaneous administration of recombinant Runx2 protein proximal to the lumbar disc in mice elicited complete degradation of intervertebral discs (IVDs). Injection of recombinant MMP1a and ADAMTS10 proteins individually induced mild to moderate degeneration of the IVDs, while co-administration of MMP1a and ADAMTS10 resulted in moderate to severe degeneration. Notably, concurrent injection of the Runx2 inhibitor CADD522 with recombinant Runx2 protein did not result in IVD degeneration in mice. Furthermore, genetic knockout of ALKBH5 and overexpression of YTHDF1 in mice, along with lipopolysaccharide (LPS) treatment to induce inflammation, did not alter the expression of Runx2, MMPs, and ADAMTSs, and no degeneration of the IVDs was observed.

Conclusion
Our study elucidates the role of ALKBH5-mediated m6A demethylation of Runx2 mRNA in activating MMPs and ADAMTSs, thereby facilitating ECM degradation and promoting the occurrence of IDD. Our findings suggest that targeting the ALKBH5/Runx2/MMPs/ADAMTSs axis may represent a promising therapeutic strategy for preventing IDD.
Impaired angiotensin II signaling in septic shock
Authors: Picod Adrien , Garcia Bruno , Van Lier Dirk , Pickkers Peter , Herpain Antoine , Mebazaa Alexandre , Azibani Feriel 
Published: June 2024

AbstractRecent years have seen a resurgence of interest for the renin–angiotensin–aldosterone system in critically ill patients. Emerging data suggest that this vital homeostatic system, which plays a crucial role in maintaining systemic and renal hemodynamics during stressful conditions, is altered in septic shock, ultimately leading to impaired angiotensin II—angiotensin II type 1 receptor signaling. Indeed, available evidence from both experimental models and human studies indicates that alterations in the renin–angiotensin–aldosterone system during septic shock can occur at three distinct levels: 1. Impaired generation of angiotensin II, possibly attributable to defects in angiotensin-converting enzyme activity; 2. Enhanced degradation of angiotensin II by peptidases; and/or 3. Unavailability of angiotensin II type 1 receptor due to internalization or reduced synthesis. These alterations can occur either independently or in combination, ultimately leading to an uncoupling between the renin–angiotensin–aldosterone system input and downstream angiotensin II type 1 receptor signaling. It remains unclear whether exogenous angiotensin II infusion can adequately address all these mechanisms, and additional interventions may be required. These observations open a new avenue of research and offer the potential for novel therapeutic strategies to improve patient prognosis. In the near future, a deeper understanding of renin–angiotensin–aldosterone system alterations in septic shock should help to decipher patients’ phenotypes and to implement targeted interventions.
Protocol for a systematic review and network meta-analysis of the use of prophylactic antibiotics in hand trauma surgery
Authors: Zhang Chen , Yusuf Suraya Mohamed , Farag Soma , Wade Ryckie George , Wormald Justin Conrad Rosen 
Published: June 2024

Abstract
Background
The use of prophylactic antibiotics in surgery is contentious. With the rise in antimicrobial resistance, evidence-based antibiotic use should be followed. This systematic review and network meta-analysis will assess the effectiveness of different antibiotics on the prevention of surgical site infection (SSI) following hand trauma surgery.

Methods and analysis
The databases Embase, MEDLINE, CINAHL and CENTRAL, ClinicalTrials.gov and the WHO International Clinical Trials Registry Platform will be searched. Abstracts will be screened by two persons independently to identify eligible studies.
This systematic review will include both randomised and non-randomised prospective comparative studies in participants with hand and/or wrist injuries requiring surgery; bite injuries will be excluded. The network meta-analysis will compare the use of different prophylactic antibiotics against each other, placebo and/or no antibiotics on the development of SSI within 30 days of surgery (or 90 days if there is an implanted device).
The Cochrane risk-of-bias tool 2 will be used to assess the risk of methodological bias in randomised controlled trials, and the Newcastle-Ottowa scale (NOS) will be used to assess the risk of bias in non-randomised studies.
A random-effects network meta-analysis will be conducted along with subgroup analyses looking at antibiotic timing, injury type, and operation location. Sensitivity analyses including only low risk-of-bias studies will be conducted, and the confidence in the results will be assessed using Confidence in Network Meta‐Analysis (CINEMA).

Discussion
This systematic review and network meta-analysis aims to provide an up-to-date synthesis of the studies assessing the use of antibiotics following hand and wrist trauma to enable evidence-based peri-operative prescribing.

Systematic review registration
PROSPERO CRD42023429618.
Social distancing stress, anxiety/depression, COVID-19 diagnosis, gender identity, and immigration status
Authors: Adzrago David , Chiangong Jolyna , Ormiston Cameron K. , Dada Oluwabunmi M. , Jones Antwan , Williams Faustine 
Published: June 2024

Abstract
Background
Strict social distancing public health measures to decrease COVID-19 spread increased social distancing stress. However, differences in social distancing stress by anxiety/depression symptoms are understudied, especially based on COVID-19 diagnosis status, gender identity, and immigration status. We examined whether the association between social distancing stress and anxiety/depression symptoms was moderated by COVID-19 diagnosis status, gender identity, and immigration status. We further examined the associations of social distancing stress with anxiety/depression symptoms, gender identity, and immigration status among individuals with and without COVID-19.

Methods
We utilized data from a national cross-sectional survey among adults aged ≥ 18 years in the United States between May 13, 2021, and January 9, 2022 (n = 5,255). Multivariable logistic regression models were used to examine the associations.

Results
The prevalence of social distancing stress was higher among individuals with COVID-19 (79.23%) than among those without COVID-19 (67.51%). We observed significant associations between social distancing stress and anxiety/depression symptoms, moderated by COVID-19 diagnosis status, immigration status, and gender identity, respectively. Anxiety/depression symptoms were associated with social distancing stress among both individuals with and without COVID-19. Gender identity and immigration status were associated with social distancing stress among only individuals without COVID-19.

Conclusions
Our findings revealed that the association between social distancing stress and anxiety/depression varied by COVID-19 diagnosis status, gender identity, and immigration status. The findings underscore the need for more targeted psychological distress strategies to reduce social distancing stress and anxiety/depression among diverse US populations, while considering the impacts of COVID-19 diagnosis status, gender identity, and immigration status.
Feasibility-sustainability study of power generation using solar energy at an industrial site: a case study from Egypt
Authors: Mostafa Noha A. , Aboelezz Amr 
Published: June 2024

Abstract
Background
Fossil fuel utilization is the biggest contributor to the emissions of greenhouse gases which are the main reason for global warming. Solar energy photovoltaic (PV) technology is one of the most rapidly rising technologies and is a sturdy candidate to replace fossil fuels due to its versatility. Egypt receives high solar intensity which makes it a perfect place for utilizing this technology. However, for the past years, the focus in Egypt was on using solar energy for residential applications, henceforth a research gap was identified in studying the feasibility of using solar energy for industrial applications in Egypt. To ensure the sustainability of this application, this feasibility study addresses technical, economic, environmental, and social aspects.

Results
A case study is investigated for utilizing solar PV panels for energy generation in Egypt at an industrial site. A food factory was studied under three scenarios. Scenario 1 is the baseline case for the other scenarios with fixed tilted PV panels and no storage, Scenario 2 is the same as Scenario 1 with difference in is the model of the PV panels with no tracking or storage system. Scenario 3 has a vertical axis tracking system. Software was used to simulate the performance of the three scenarios for 25 years. Results have shown that Scenario 1 and Scenario 2 had close values of the annual energy production. However, Scenario 3 produces 2047 MWh annually which is considerably higher. Finally, a sensitivity analysis is carried out to test the effect of some economic parameters on the financial feasibility.

Conclusions
All the three scenarios are found to be feasible. Scenario 1 has the shortest discounted payback period with a net present value of 414,110.12 USD, a nominal levelized cost of energy of 0.022 USD/kWh, and avoided CO2 emissions of 14,898.993 tons. Although Scenario 3 has higher costs, it has higher energy production and better impact on the environment with 18,891.435 tons of avoided CO2 emissions. The paper concluded that a generalization could be done about using solar PV systems in Egypt for energy generation to be sustainable and feasible technically, economically, and environmentally.
Environmental trade-offs of (de)centralized renewable electricity systems
Authors: Reutter F. , Lehmann P. 
Published: June 2024

Abstract
Background
Renewable energies are key to reduce CO2 emissions and other environmental impacts of fossil-fueled electricity generation. However, renewable energy systems can also cause negative environmental effects. In this paper, we analyze the potential environmental trade-offs associated with different spatio-technical (de)centralization options for a renewable electricity system. For this purpose, we first review the potential environmental life cycle impacts of key technologies for renewable electricity systems. Subsequently, we develop a framework identifying which factors determine actual environmental effects of renewable electricity systems. We apply the framework to four basic spatio-technical (de)centralization options for the future Germany electricity system.

Results
Our analysis shows that all (de)centralization options are associated with potential environmental trade-offs. We find that the (de)centralization of the system is a relevant factor determining these trade-offs. For instance, the two more centralized options considered have lower environmental impacts related to PV, whereas the two more decentralized options have lower environmental impacts related to grid infrastructure. However, we also find that the trade-offs depend on the specific way (de)centralization is pursued. For instance, only in one of the two considered more decentralized development options, there is a potential environmental trade-off between higher impacts related to battery storage and lower impacts related to offshore wind power.

Conclusions
Our analysis reveals that the spatio-technical (de)centralization of a renewable electricity system plays a role for its environmental trade-offs while further factors like the institutional and stakeholder management in place also shape the environmental trade-offs. Policy makers should acknowledge the identified potential environmental trade-offs and their influencing factors when making policies favoring certain spatio-technical (de)centralization options.
Ageism, welfare, and the energy transition: a comparative analysis of the perceptions among the elderly in Poland and Norway
Authors: Szulecki Kacper , Neerland Maria Aspen , Tomter Håkon , Wæringsaasen Cecilie A. Blomberg , Żuk Paweł , Żuk Piotr 
Published: June 2024

Abstract
Background
One of the potential dimensions on which exclusion and injustice may occur in energy transitions is age. Age-based patterns of exclusion—ageism—has recently been conceptualized in the context of decarbonization as energy ageism. This paper offers a comparative empirical analysis of the senior citizens’ outlook towards an imminent energy transition as well as the impact of energy poverty in two European countries: Norway and Poland.

Results
Drawing on interviews and focus groups with Polish and Norwegian seniors, we present the differences and similarities between the two countries, and the determinants of energy ageism, as well as the concept’s overall applicability and empirical usefulness. We find that socioeconomic conditions outweigh ageism, that is, the resilience of senior citizens in dealing with energy poverty during a transition is conditioned by their material standing and welfare state robustness rather than age based. An important factor is household heating technology, combined with economic vulnerability can push some individuals into energy poverty, while others using alternative sources of heat can navigate through energy crises unscathed.

Conclusions
We note the importance of mainstreaming social inclusion considerations in energy policy and of targeted digital competence building which can enhance senior citizen integration in the energy transition. Lower levels of digital competences among senior citizens certainly play a role and need to be addressed with education programs to increase participation. In both countries, household heating is a major issue and heating sources are strong predictors of energy poverty and regulatory measures and subsidies should be designed at national, regional, and municipal level to assist vulnerable groups in this area.
Student entrepreneurship competency and mindset: examining the influence of education, role models, and gender
Authors: Alkaabi Khaula , Senghore Salieu 
Published: June 2024

AbstractThis research aims to explore the interplay among entrepreneurial education, role models, and gender on students' entrepreneurial competence and mindset. Using purposive sampling, 306 participants enrolled in entrepreneurship and innovation-related courses at UAEU completed an online survey. The research used statistical analyses, including Spearman's Rank Correlations, Cramer's V correlation, Mann–Whitney U, and multiple linear regression, to explore variable relationships. Findings indicate that access to entrepreneurial role models is associated with higher competence, emphasizing resource mobilization and fostering a growth mindset. Active engagement in entrepreneurship courses correlates with enhanced innovative thinking and networking. Despite gender-based disparities, statistical analysis indicates minimal impact on students' ability to generate novel business ideas. This study contributes insights to entrepreneurship education literature, offering practical implications for educators and institutions.