bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phase 3 randomized COMMODORE 2 trial: Crovalimab versus eculizumab in patients with paroxysmal nocturnal hemoglobinuria naive to complement inhibition
Authors: Röth Alexander , He Guangsheng , Tong Hongyan , Lin Zenghua , Wang Xiaoqin , Chai‐Adisaksopha Chatree , Lee Je‐Hwan , Brodsky Andres , Hantaweepant Chattree , Dumagay Teresita E. , Demichelis‐Gómez Roberta , Rojnuckarin Ponlapat , Sun Jing , Höglund Martin , Jang Jun Ho , Gaya Anna , Silva Fernando , Obara Naoshi , Kelly Richard J. , Beveridge Leigh , Buatois Simon , Chebon Sammy , Gentile Brittany , Lundberg Pontus , Sreckovic Sasha , Nishimura Jun‐ichi , Risitano Antonio , Han Bing 
Published: June 2024

AbstractCrovalimab is a novel C5 complement inhibitor that enables rapid and sustained C5 inhibition with subcutaneous, low‐volume self‐administration every 4 weeks. COMMODORE 2 (NCT04434092) is a global, randomized, open‐label, multicenter, phase 3 trial evaluating the non‐inferiority of crovalimab versus eculizumab in patients with paroxysmal nocturnal hemoglobinuria not previously treated with C5 inhibition. C5 inhibitor‐naive patients with lactate dehydrogenase (LDH) ≥2 × upper limit of normal (ULN) were randomized 2:1 to crovalimab or eculizumab. Co‐primary efficacy endpoints were proportion of patients with hemolysis control (centrally assessed LDH ≤1.5 × ULN) and proportion with transfusion avoidance. Secondary efficacy endpoints were proportions of patients with breakthrough hemolysis, stabilized hemoglobin, and change in FACIT‐Fatigue score. The primary treatment period was 24 weeks. Two hundred and four patients were randomized (135 crovalimab; 69 eculizumab). Crovalimab was non‐inferior to eculizumab in the co‐primary endpoints of hemolysis control (79.3% vs. 79.0%; odds ratio, 1.0 [95% CI, 0.6, 1.8]) and transfusion avoidance (65.7% vs. 68.1%; weighted difference, −2.8 [−15.7, 11.1]), and in the secondary efficacy endpoints of breakthrough hemolysis (10.4% vs. 14.5%; weighted difference, −3.9 [−14.8, 5.3]) and hemoglobin stabilization (63.4% vs. 60.9%; weighted difference, 2.2 [−11.4, 16.3]). A clinically meaningful improvement in FACIT‐Fatigue score occurred in both arms. Complete terminal complement activity inhibition was generally maintained with crovalimab. The safety profiles of crovalimab and eculizumab were similar with no meningococcal infections. Most patients who switched from eculizumab to crovalimab after the primary treatment period preferred crovalimab. These data demonstrate the positive benefit–risk profile of crovalimab.
Blepharophimosis with intellectual disability and Helsmoortel‐Van Der Aa Syndrome share episignature and phenotype
Authors: Sarli Camilla , van der Laan Liselot , Reilly Jack , Trajkova Slavica , Carli Diana , Brusco Alfredo , Levy Michael A. , Relator Raissa , Kerkhof Jennifer , McConkey Haley , Tedder Matthew L. , Skinner Cindy , Alders Mariëlle , Henneman Peter , Hennekam Raoul C. M. , Ciaccio Claudia , D'Arrigo Stefano , Vitobello Antonio , Faivre Laurence , Weber Sacha , Vincent‐Devulder Aline , Perrin Laurence , Bourgois Alexia , Yamamoto Toshiyuki , Metcalfe Kay , Zollino Marcella , Kini Usha , Oliveira Daniela , Sousa Sergio B. , Williams Denise , Cappuccio Gerarda , Sadikovic Bekim , Brunetti‐Pierri Nicola 
Published: June 2024

AbstractBlepharophimosis with intellectual disability (BIS) is a recently recognized disorder distinct from Nicolaides‐Baraister syndrome that presents with distinct facial features of blepharophimosis, developmental delay, and intellectual disability. BIS is caused by pathogenic variants in SMARCA2, that encodes the catalytic subunit of the superfamily II helicase group of the BRG1 and BRM‐associated factors (BAF) forming the BAF complex, a chromatin remodeling complex involved in transcriptional regulation. Individuals bearing variants within the bipartite nuclear localization (BNL) signal domain of ADNP present with the neurodevelopmental disorder known as Helsmoortel‐Van Der Aa Syndrome (HVDAS). Distinct DNA methylation profiles referred to as episignatures have been reported in HVDAS and BAF complex disorders. Due to molecular interactions between ADNP and BAF complex, and an overlapping craniofacial phenotype with narrowing of the palpebral fissures in a subset of patients with HVDAS and BIS, we hypothesized the possibility of a common phenotype‐specific episignature. A distinct episignature was shared by 15 individuals with BIS‐causing SMARCA2 pathogenic variants and 12 individuals with class II HVDAS caused by truncating pathogenic ADNP variants. This represents first evidence of a sensitive phenotype‐specific episignature biomarker shared across distinct genetic conditions that also exhibit unique gene‐specific episignatures.
Aerodynamically Levitated Droplets as Small‐Scale Chemical Reactors and Liquid Microprinters
Authors: Jia Yankai , Sobolev Yaroslav I. , Cybulski Olgierd , Klucznik Tomasz , Quintana Cristóbal , Ahumada Juan Carlos , Grzybowski Bartosz A. 
Published: June 2024

AbstractA thin liquid film spread over the inner surface of a rapidly rotating vial creates an aerodynamic cushion on which one or multiple droplets of various liquids can levitate stably for days or even weeks. These levitating droplets can serve as wall‐less (“airware”) chemical reactors that can be merged without touching—by remote impulses—to initiate reactions or sequences of reactions at scales down to hundreds of nanomoles. Moreover, under external electric fields, the droplets can act as the world's smallest chemical printers, shedding regular trains of pL or even fL microdrops. In one modality, the levitating droplets operate as completely wireless aliquoting/titrating systems delivering pg quantities of reagents into the liquid in the rotating vial; in another modality, they print microdroplet arrays onto target surfaces. The “airware”, levitated reactors are inexpensive to set up, remarkably stable to external disturbances and, for printing applications, require operating voltages much lower than in electrospray, electrowetting, or ink jet systems.
Revolutionizing electrospinning: Sustainable solutions through deep eutectic solvents in biopolymer processing
Authors: Frąckowiak Patrycja , Jędrzejczak Eryk , Kaspryszyn Filip , Jesionowski Teofil , Wysokowski Marcin 
Published: June 2024

AbstractElectrospinning is currently one of the most used techniques for obtaining various types of nanofibers. In the era of sustainable development, it is essential to utilize environmentally friendly raw materials and solvents. This review summarizes the current state of the art in biopolymer electrospinning using deep eutectic solvents. To date, there have not been too many studies on the simultaneous application of these mixtures and biopolymers in the electrospinning process. Nevertheless, the use of natural origin raw materials and the replacement of conventional organic solvents with deep eutectic solvents appear promising in this technique due to many attractive properties of both the mixtures and biopolymers. This article aims to inspire scientists to further research in this direction, related to the development of electrospinning techniques aimed at reducing the negative environmental impact.
Determination of valsartan and pitavastatin using synchronous spectrofluorimetry and augmented least squares chemometric models: A comparative study with greenness and blueness assessment
Authors: Almalki Atiah H. , Abduljabbar Maram H. , Alzhrani Rami M. , Alosaimi Manal E. , Serag Ahmed 
Published: June 2024

AbstractHypertension and hyperlipidemia are two common conditions that require effective management to reduce the risk of cardiovascular diseases. Among the medications commonly used for the treatment of these conditions, valsartan and pitavastatin have shown significant efficacy in lowering blood pressure and cholesterol levels, respectively. In this study, synchronous spectrofluorimetry coupled to chemometric analysis tools, specifically concentration residual augmented classical least squares (CRACLS) and spectral residual augmented classical least squares (SRACLS), was employed for the determination of valsartan and pitavastatin simultaneously. The developed models exhibited excellent predictive performance with relative root mean square error of prediction (RRMSEP) of 2.253 and 2.1381 for valsartan and pitavastatin, respectively. Hence, these models were successfully applied to the analysis of synthetic samples and commercial formulations as well as plasma samples with high accuracy and precision. Besides, the greenness and blueness profiles of the determined samples were also evaluated to assess their environmental impact and analytical practicability. The results demonstrated excellent greenness and blueness scores with AGREE score of 0.7 and BAGI score of 75 posing the proposed method as reliable and sensitive approach for the determination of valsartan and pitavastatin with potential applications in pharmaceutical quality control, bioanalytical studies, and therapeutic drug monitoring.
Metagenomics analysis yields assembled genomes from prokaryotic anaerobes with polymer‐degrading potential
Authors: Blair Elaina M. , Brown Jennifer L. , Li Dong , Holden Patricia A. , O'Malley Michelle A. 
Published: June 2024

AbstractAnaerobic microbial communities are often highly degradative, such as those found in the herbivore rumen and large‐scale anaerobic digesters. Since the microbial communities in these systems degrade recalcitrant organic polymers, we hypothesize that some microbes in anaerobic environments may be involved in man‐made plastic association, deformation, or even breakdown. While efforts have been put toward characterizing microbial communities, many microbes remain unidentified until they can be sufficiently cultivated to generate enough genetic material to assemble high‐quality metagenome assemblies and reference genomes. In this study, microbial consortia from goat fecal pellets and anaerobic digester sludge were cultivated for over 6 weeks to assemble metagenomes from novel anaerobic taxa with potential degradative activity. To select for microbes with potential plastic‐degrading abilities, plastic strips were included in culture, though the presence of plastic did not appear to enrich for particularly degradative consortia, yet it did select for novel species that otherwise may not have been characterized. Whole‐genome shotgun sequencing enabled assembly of 72 prokaryotic metagenome‐assembled genomes (MAGs) with >90% completion, <5% contamination, and an N50 >10,000 bp; 17 of these MAGs are classified as novel species given their lack of similarity to publicly available genomes and MAGs. These 72 MAGs vary in predicted carbohydrate‐degrading abilities, with genes predicted to encode fewer than 10 or up to nearly 400 carbohydrate‐active enzymes. Overall, this enrichment strategy enables characterization of less abundant MAGs in a community, and the MAGs identified here can be further mined to advance understanding of degradative anaerobic microbial consortia.
Navigating consumption streams: Toward a utilitarian–hedonic perspective on subscription box customer journeys
Authors: Banerji Ishani , Gupta Aditya , Ketron Seth 
Published: June 2024

AbstractGiven persistent challenges faced by subscription box retailers in acquiring and retaining customers, there is an urgent need to understand decision‐making and sources of value at each stage of the subscription box customer journey. As extant research has frequently focused on the customers' decision to subscribe, relatively little work has explored why they choose to continue or cancel their subscriptions. We contend that a way forward in understanding subscription box customer journeys is to adopt a utilitarian–hedonic perspective when looking at each stage of such journeys. We conduct phenomenological in‐depth interviews with 26 subscription box customers and use the thematic insights to introduce a processual framework of subscription boxes as consumption steams. We posit that the subscription box customer journey comprises three key decision stages (initiation, immersion, and egression) with each stage characterized by distinct themes that add to or subtract from the utilitarian and hedonic value at that stage. Collectively, these themes provide a more nuanced understanding of the factors that shape customer decisions to start, renew, or cancel a subscription box. We conclude by noting how our findings contribute to marketing theory and practice and suggest some avenues for future research.
Chemical Composition, Antifungal, Antioxidant, and Hemolytic Activities of Moroccan Thymus capitatus Essential Oil
Authors: Amakran Amina , Hamoudane Meriem , Pagniez Fabrice , Lamarti Ahmed , Picot Carine , Figueredo Gilles , Nhiri Mohamed , Le Pape Patrice 
Published: June 2024

AbstractThis study aimed to define the chemical composition of Moroccan Thymus capitatus essential oil, and to investigate its in vitro antioxidant and antifungal activities against human pathogenic fungi. Chemical analysis using GC‐FID and GC‐MS system revealed 28 constituents, representing 99 % of total compounds. Oxygenated monoterpenes represented the highest proportion (79.79 %), among which carvacrol (75.73 %) was the predominant compound, followed by linalol (2.26 %). Monoterpene hydrocarbons represented the second major fraction (16.29 %): within them, the predominant constituents were γ‐terpinene (5,55 %), ρ‐cymene (5,50 %), and β‐caryophyllene (2.73 %). Antioxidant activity was performed by DPPH scavenging, β‐carotene bleaching inhibition, and ferric reducing power. T. capitatus revealed pronounced DPPH radical scavenging activity (IC50=110.53 μg mL−1), strong ferric reducing ability (EC50=644.4 μg mL−1), and a remarkable degree of protection against lipid peroxidation during β‐carotene bleaching inhibition (IC50=251.76 μg mL−1). Antifungal activity was carried out against Candida, Aspergillus, and Rhizopus species by microdilution method. T. capitatus exhibited potent anticandidal activity (MIC=125–500 μg mL−1) and strong inhibition against filamentous fungi (MIC=250‐500 μg mL−1). Its hemolytic activity against human erythrocytes had a low toxic effect at concentrations lower than 1250 μg mL−1. The useful antioxidant properties and broad antifungal effect of T. capitatus EO confirm its considerable potential for the food industry and for phytopharmaceutical production.
Abietane‐Type Diterpenoids: Insights into Structural Diversity and Therapeutic Potential
Authors: Sargazifar Zahra , Ghorbanian Charami Danial , Esmaeilzadeh Kashi Marziyeh , Asili Javad , Shakeri Abolfazl 
Published: June 2024

AbstractThe abietane‐type diterpenoids are among the most significant diterpene subsets found in hundreds of plant species belonging to various families. Among which, the members of the genus Salvia and Euphorbia are rich in abietane diterpenoids. Because of the chemical diversity and notable bioactivities, such as anticancer, antiinflammatory, antimicrobial, and antioxidant activities, they are attractive. Herein, recent advances in the isolation and characterization of abietanes from natural sources, as well as their biological activities, from 2015 up to 2024 are reviewed. During this time, over 300 abietanes with diverse structures have been discovered.
Streptomyces Endophytes in Edible Plants: New Insights into their Chemistry and Health Benefits
Authors: Moussa Ashaimaa Y. 
Published: June 2024

AbstractStreptomyces is the largest source of microbial antibiotics with about 50 % of marketed antimicrobial drugs originating from this genus. Endophytic streptomyces are the link between medicinal plants and the microbial world. Endophytic Streptomyces in edible plants were not targeted before despite their uniqueness and importance. In this review, we analyzed the chemical diversity of more than 150 compounds belonging to endophytic Streptomyces chemical classes such as alkaloids, polyketides, peptides, macrolides and terpenes and their biological activities. This analysis showed a dominant antimicrobial effect for most of the isolated compounds and highlighted an underestimated diversity to be studied or repurposed for other biological activities. Return to edible plants use and conducting toxicity studies to rationalize their nutraceutical potential based on their beneficial endophytes is urged. Although there are many studies for non‐vertebrates, the nutraceutical potential of these plants is expected to improve the gut microbiota since they are enriched with bioactive compounds from streptomyces species. This is the first review to discuss edible plants associated streptomyces, and we prospect that many studies will follow to unravel the mysterious health benefits of streptomyces in the human microbiome and encourage the revival of a correct lifestyle for the sake of a healthier microbiome.
Recent Advancements in the Development of Graphene‐Based Materials for Catalytic Applications
Authors: Gao Kai , Sun Sai , Zhang Bin 
Published: June 2024

AbstractGraphene, a two‐dimensional material, presents unprecedented opportunities for catalytic applications, attributable to its unique planar structure, outstanding optical transmittance, superior electronic conductivity and mobility, extensive specific surface area, and elevated chemical stability, coupled with its tunability. In recent years, graphene‐based catalysts have garnered significant research attention and interest. This review aims to elucidate recent advancements in the synthesis of graphene‐based catalysts. It encompasses discussions on heteroatom‐doped metal‐free graphene catalysts, graphene composite catalysts (including graphene/metal and graphene/metal oxide composites), and their applications in various catalytic domains such as electrocatalysis, photocatalysis, and catalysis of organic reactions.
Spiritual competent orientation as ground for effective clinical supervision: Strategies for implementation
Authors: Gutierrez Daniel , Dorais Stephanie , Cashwell Craig S. 
Published: June 2024

AbstractOne of the aims of clinical supervision is to ensure counselors meet the ethical obligations of the counseling profession, which include religious and spiritual (R/S) competence. To date, however, there has been no framework for the supervision of R/S competence in the research literature. We use an extension of the spiritually competent orientation model to illustrate how to conduct supervision that builds R/S competence in practice.
Is the E/Z Iminium Ratio a Good Enantioselectivity Predictor in Iminium Catalysis?
Authors: Hellinghuizen Matthijs A. , Franceschi Pietro , Roithová Jana 
Published: June 2024

AbstractDeveloping new enantioselective reactions is an important part of chemical discovery but requires time and resources to test large arrays of potential reaction conditions. New techniques are required to analyse many different reactions quickly and efficiently. Mass spectrometry is a high‐throughput method; when combined with ion‐mobility spectrometry, this technique can monitor diastereomeric reaction intermediates and thus be a handle to study enantioselective reactions. Through this technique and others, it was noted before that in the organocatalytic 1,4‐addition to α,β‐unsaturated aldehydes, the abundance of initial diastereomeric intermediates correlates strongly to that of the final enantiomeric products. This work determines isomeric abundance for various catalysts and aldehydes and uses it to predict the enantiomeric excess of two control reactions. The prediction matches well for one reaction but does not predict the obtained results for the second. This finding confirms that the E/Z ratio of the iminium intermediates can be used as a predictor for some reactions, but the kinetics of the following steps can dramatically change the true enantioselectivity.
Insulin resistance during androgen deprivation therapy in men with prostate cancer
Authors: Basaria Shehzad , Taplin Mary‐Ellen , McDonnell Marie , Simonson Donald C. , Lin Alexander P. , Dufour Alyssa B. , Habtemariam Daniel , Nguyen Paul L. , Ravi Praful , Kibel Adam S. , Sweeney Christopher J. , D’Amico Anthony V. , Roberts Daniel A. , Xu Wenxin , Wei Xiao X. , Sunkara Rajitha , Choudhury Atish D. , Mantia Charlene , Beltran Himisha , Pomerantz Mark , Berchuck Jacob E. , Martin Neil E. , Leeman Jonathan E. , Mouw Kent W. , Kilbridge Kerry E. , Bearup Richelle , Kackley Hannah , Kafel Hussein , Huang Grace , Reid Kieran F. , Storer Thomas , Braga‐Basaria Milena , Travison Thomas G. 
Published: June 2024

AbstractBackgroundAndrogen deprivation therapy (ADT) in prostate cancer (PCa) has been associated with development of insulin resistance. However, the predominant site of insulin resistance remains unclear.MethodsThe ADT & Metabolism Study was a single‐center, 24‐week, prospective observational study that enrolled ADT‐naive men without diabetes who were starting ADT for at least 24 weeks (ADT group, n = 42). The control group comprised men without diabetes with prior history of PCa who were in remission after prostatectomy (non‐ADT group, n = 23). Prevalent diabetes mellitus was excluded in both groups using all three laboratory criteria defined in the American Diabetes Association guidelines. All participants were eugonadal at enrollment. The primary outcome was to elucidate the predominant site of insulin resistance (liver or skeletal muscle). Secondary outcomes included assessments of body composition, and hepatic and intramyocellular fat. Outcomes were assessed at baseline, 12, and 24 weeks.ResultsAt 24 weeks, there was no change in hepatic (1.2; 95% confidence interval [CI], −2.10 to 4.43; p = .47) or skeletal muscle (−3.2; 95% CI, −7.07 to 0.66; p = .10) insulin resistance in the ADT group. No increase in hepatic or intramyocellular fat deposition or worsening of glucose was seen. These changes were mirrored by those observed in the non‐ADT group. Men undergoing ADT gained 3.7 kg of fat mass.ConclusionsIn men with PCa and no diabetes, 24 weeks of ADT did not change insulin resistance despite adverse body composition changes. These findings should be reassuring for treating physicians and for patients who are being considered for short‐term ADT.
Bi2O2CO3/g‐C3N4 Catalyst for Photocatalytic Coupling of Benzylamine under Mild Conditions
Authors: Teng Yunan , Tan Zhonghao , Zhao Yingzhe , Su Zhuizhui , Li Meiling , Zhang Zixuan , Zhang Jianling 
Published: June 2024

AbstractThe photocatalytic conversion of benzylamine into imine is promising for industrial production and environmental protection. To develop photocatalysts with desirable compositions and microstructures is key to achieve high activity and selectivity. Here we propose the immobilization of Bi2O2CO3 on g‐C3N4 for the photocatalytic conversion of benzylamine. The Bi2O2CO3/g‐C3N4 catalyst possesses improved light absorption capacity, electron transmission rate and reduced electron‐hole recombination than pure Bi2O2CO3. It can efficiently catalyze benzylamine coupling reaction under mild conditions, i. e., at room temperature, with air as oxidant and no additional oxidant involved. The maximum turnover frequency value of N‐benzylbenzaldimine reaches 1555.3 μmol g−1 h−1 under this condition. The Bi2O2CO3/g‐C3N4 catalyst has potential in other photocatalytic reactions.