bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of claustrum in incubation of opioid seeking after electric barrier-induced voluntary abstinence in male and female rats
Authors: Negishi Kenichiro , Duan Ying , Batista Ashley , Pishgar Mona S. , Tsai Pei-Jung , Caldwell Kiera E. , Claypool Sarah M. , Reiner David J. , Madangopal Rajtarun , Bossert Jennifer M. , Yang Yihong , Shaham Yavin , Fredriksson Ida 
Published: February 2025

We previously reported that ventral subiculum (vSub) activity is critical to incubation of oxycodone seeking after abstinence induced by adverse consequences of drug seeking. Here, we studied the role of claustrum, a key vSub input, in this incubation.We trained male and female rats to self-administer oxycodone for 2 weeks and then induced abstinence by exposing them to an electric barrier for 2 weeks. We used retrograde tracing (cholera toxin B subunit; CTb) plus the activity marker Fos to identify projections to vSub activated during ‘incubated’ relapse (abstinence day 15). We then used muscimol+baclofen (GABAa+GABAb receptor agonists) reversible inactivation to determine causal role of claustrum in incubation and the behavioral and anatomical specificity of this role. We also used muscimol+baclofen in an anatomical disconnection procedure to determine the causal role of claustrum-vSub connections in incubation. Finally, we analyzed an existing functional MRI dataset to determine if functional connectivity changes in claustrum-related circuits predict incubation of oxycodone seeking.Claustrum neurons projecting to vSub were activated during relapse tests after electric barrier-induced abstinence. Inactivation of claustrum but not areas dorsolateral to claustrum decreased incubation of oxycodone seeking after electric barrier-induced abstinence; claustrum inactivation had no effect on incubation after food choice-induced abstinence. Both ipsilateral and contralateral inactivation of claustrum-vSub projections decreased incubation after electric barrier-induced abstinence. Functional connectivity changes in claustrum-cortical circuits during electric barrier-induced abstinence predicted incubated oxycodone relapse.Our study identified a novel role of claustrum in relapse to opioid drugs after abstinence induced by adverse consequences of drug seeking.Significance statementWe recently reported that vSub is critical to incubation of oxycodone craving after abstinence induced by adverse consequences of drug seeking. Here, we first showed that claustrum projections to vSub are active during tests for incubation of oxycodone seeking after electric barrier-induced abstinence. Next, we used pharmacological inactivation to test the causal roles of claustrum and its anatomical connections with vSub in incubation. Using an existing functional MRI dataset, we also tested if functional connectivity changes in claustrum-related circuits predicted ‘incubated’ oxycodone relapse after electric barrier-induced abstinence. Our data suggest that claustrum- and claustrum-related circuits contribute to relapse after voluntary abstinence induced by adverse consequences to drug seeking but not abstinence induced by availability of alternative non-drug rewards.
Parathyroid carcinoma and pheochromocytoma in a patient with neurofibromatosis type 1: a rare association
Authors: Leite Jardelina Brena Rocha , De Freitas Nicole Ramalho , Cardoso Rafaella Nelice de Holanda , Trovão Diniz Erik , Ferreira Gabriel Rodrigues de Assis , Costa e Alvim Fernão Henrique , Madruga Camila Ribeiro Coutinho , Garrido Ana Carolina Thé , Albuquerque Luciano , Gadelha Patricia Sampaio , Lyra Ruy , Vilar Lucio 
Published: February 2025

Summary
The case report outlines a 33-year-old woman with neurofibromatosis type 1 (NF1) presenting complex symptomatology including a cervical mass, bone pain and significantly elevated calcium and parathyroid hormone levels, indicative of parathyroid carcinoma accompanied by cystic fibrous osteitis. Intriguingly, an incidental finding of an adrenal nodule prompted investigation, leading to the diagnosis of pheochromocytoma. Surgical intervention confirmed the presence of pheochromocytoma and parathyroid carcinoma. Genetic analysis corroborated NF1 with a pathogenic variant in the NF1 gene. The patient’s clinical manifestations, coupled with the presence of café-au-lait spots and axillary freckles, supported the diagnosis of NF1. This case not only highlights the challenging diagnostic landscape of NF1 but also underscores the rarity of the co-occurrence of parathyroid carcinoma and pheochromocytoma within the context of NF1. It emphasizes the necessity for heightened clinical suspicion and comprehensive evaluation in patients with NF1, particularly in those presenting with endocrine abnormalities. Further investigation into the underlying mechanisms linking these conditions is warranted to elucidate their pathophysiological interplay and inform optimal therapeutic strategies for affected individuals. This case underscores the importance of multidisciplinary collaboration in the management of complex NF1-associated manifestations, with an emphasis on early detection and tailored intervention to optimize patient outcomes.

Learning points

Rare but real: multiple endocrine tumors can coexist in patients with neurofibromatosis type 1 (NF1), not only pheochromocytoma.

Early detection matters: prompt diagnosis and a multidisciplinary approach are crucial for managing NF1 patients presenting with symptoms suggestive of these rare endocrine tumors.

Genetic insights guide care: genetic testing aids in confirming NF1 and guiding treatment decisions, emphasizing the role of genetics in personalized medicine for NF1 patients.
A novel approach to intraoperative diagnosis of primary pigmented nodular adrenocortical disease
Authors: Wijesekara Pathirana Nethrani Sameera , Udayakumara Hasitha , Banagala A S K , Sumanatilleke M R , Damayanthi A V T , Constantine S R , Katulanda G W , Thowfeek Z T M , Sasikanth T , Pathmanathan S 
Published: February 2025

Summary
Cushing syndrome (CS) is an endocrine disorder with far-reaching complications that extend beyond the disease remission. Diagnosis of the aetiology of CS can be challenging, whether it is dependent or independent of adrenocorticotrophic hormone (ACTH). Here, we describe a case of ACTH-independent CS due to primary pigmented nodular adrenocortical disease (PPNAD) in a 33-year-old female patient with several complications of CS, including diabetes, hypertension, osteoporosis and severe depression with suicidal ideation. In this case, following the demonstration of ACTH independence of CS, it was challenging to localise the lesion as there were bilateral adrenal lesions. Furthermore, preoperative efforts in localisation in the form of adrenal venous sampling (AVS) failed. However, the diagnosis of PPNAD was confirmed using an intraoperative frozen section and macroscopy, and the patient underwent bilateral adrenalectomy during the same surgery. This case highlights a novel approach to diagnosing and managing PPNAD intraoperatively in a resource-limited setting where preoperative localisation studies have failed.

Learning points

AVS can assist in localising the functional lesion in ACTH-independent CS due to bilateral adrenal lesions.

PPNAD diagnosis can be made through a frozen section intraoperatively, thus giving further confirmation needed to justify bilateral adrenalectomy in such cases.
Actividad física y su relación con el consumo de alcohol, tabaco, y con variables asociadas a la salud mental en estudiantes universitarios: Un estudio transversal
Authors: Arboleda-Serna Víctor Hugo , Vargas-Romero Astrid Viviana , Gutiérrez Macías Liliana 
Published: February 2025

Introduction: Academic responsibilities modifies severals behaviors in students that affect their health.
Objective: Identify the levels of physical activity (PA), alcohol, smoking, anxiety, depression, and health-related quality of life (HRQoL) in university students. Additionally, explore associations between PA with the other variables.
Methods: Cross-sectional and correlational study in 717 students that used a simple random sample. The Global Physical Activity Questionnaire, CAGE Questionnaire, Global Adult Tobacco Survey, Generalized Anxiety Disorder-7, Patient Health Questionnaire-9, and SF-12 were applied.
Results: 42.4% presented a high level of PA, 23.8% a moderate level, and 33.8% a low level, there were statistically significant differences by sex (p<0.05), with a moderate association (Cramér's v = 0.20). 30.6% showed AC problems and 13.1% alcohol dependence. 92.2% do not smoke, 7.0% smoke, and 0.8% did not respond. There was no correlation between PA and CA, and PA and smoking (p>0.05). 43.5% had mild anxiety, 31.5% had no anxiety, 13.8% had moderate anxiety, and 11.2% had severe anxiety. 15.7% presented moderately severe and severe depression (grouped), 34.7% mild and 35.7% without depression. There was a relationship between PA and depression (p<0.05; Cramér's v = 0.13). PA and HRQoL showed statistically significant associations (p<0.05) of weak magnitude (<0.30).
Conclusions: The results suggest thinking about strategies to increase PA with emphasis on women. It is important to take action to treat alcohol consumption and alcohol dependence problems, as well as anxiety and depression given the proportions found. The mental component of HRQoL should be treated with attention since it showed a decrease of 7.5 points below the reference population average.
Comparison of Laparoscopic Intraperitoneal Onlay Mesh Repair (IPOM plus) vs Open Rives–Stoppa (RS) Repair for Ventral and Incisional Hernia
Authors: Havrylov Hryhorii O. , Shulyarenko Oleg V. , Yosypenko Mykhaylo O. 
Published: February 2025

Aim. To compare the effects of laparoscopic intraperitoneal onlay mesh plus repair versus open Rives–Stoppa repair for abdominal wall hernias.Materials and methods. A total of 99 patients with midline primary, umbilical or incisional hernias who underwent surgery in “Medikom” clinic and Kyiv city hospital #5 in the period from 2016 to 2022 were involved in the study. The group I included 50 patients who underwent intraperitoneal onlay mesh (IPOM) plus, and the group II 49 patients who underwent open Rives–Stoppa (RS) repair.Result. Both groups were comparable in mean age, gender, body mass index, patient distribution based on hernia type, defect size, ASA score distribution (p > 0.05).The operating time in minutes was 75.36 ± 4.99 in group I and 97.85 ± 6.5 in group II (p < 0.05). The blood loss in IPOM plus approach group is on average in 2 times less than in open RS technique (p < 0.05). The pain score 12 hours after surgery was 5.5 ± 0.64 in group I comparing to 7.26 ± 0.78 in group II (p < 0.05). The pain score 24 hours after surgery was 4.46 ± 0.7 in group I comparing to 4.95 ± 0.61 in group II (р < 0.05). The pain score 8 days after surgery in group I was on average in 1.46 times less than in group II (p < 0.05).No significant difference was found in incidence of early complications between two groups (p > 0.05).47 (94%) patients of group I and 46 (93.87%) patients of group II were evaluated over 22 months follow-up period. No one complication was noted.Conclusions. IPOM plus approach for the ventral and incisional hernias repair is a viable and relatively more safe operation by comparing with open RS repair. IPOM plus approach takes significantly in 1.3 less time as compared open RS technique.
Ankle and Distal Tibia Megaprostheses in Orthopedic Oncology: A Report of Two Cases and Review of the Literature
Authors: Ipponi Edoardo , Cordoni Martina , Bechini Elena , Gentili Fabrizia , Cosseddu Fabio , D'Arienzo Antonio , Andreani Lorenzo 
Published: February 2025

Background: The distal tibia is one of the rarest sites for the onset of malignant and locally aggressive bone tumors. When diagnosed, these lesions should undergo surgical resection with wide margins to eradicate the disease. In the era of limb-sparing surgery, several reconstructive approaches have been proposed and described in modern literature for reconstructing the distal tibia and the ankle. The 3D-printed custom-made prostheses represent an innovative and promising reconstructive option. Several authors highlighted that despite being expensive and prone to some complications such as talar collapse or loosening and soft tissue necrosis, the megaprostheses of the distal tibia could lead to good functional outcomes, also allowing a better range of motion compared to the most common arthrodesis.Materials and methods: We report two cases who suffered from malignant bone tumors localized in the distal tibia and treated with wide resections and the implant of 3D printed custom-made megaprostheses to replace the distal tibia and the ankle.Results: Both patients had excellent functional results (MSTS 30/30) one year after surgery. No local recurrence occurred during the patients’ latest follow-up.Conclusions: Our results support the effectiveness of custom-made implants in replacing the distal tibia and the ankle in orthopedic oncology.
Werner Syndrome Caused by Homozygous Frameshift Variant c.1578del in WRN
Authors: Druta Jovita Patricija , Petraitytė Gunda , Sasnauskienė Aušra , Preikšaitienė Eglė 
Published: February 2025

Background. Progerias are rare hereditary genetic disorders that cause the onset of aging to occur earlier than generally expected, which initiates the progression of many age-related diseases. Syndromes assigned to this group are usually a compound disturbance of multiple systems. Werner syndrome is among a few well described premature aging disorders associated with a higher likelihood of malignancies.Clinical case. We present a 45-year-old man with a history of painful muscle spasms, general muscle pain and weakness. There was a progression of contractures of the plantar tendons, as well as the atrophy of the subcutaneous adipose tissue of the extremities. The patient was initially diagnosed with secondary small fiber sensory polyneuropathy and myotonia, but further genetic testing revealed the homozygous pathogenic variant c.1578del in the WRN gene associated with Werner syndrome.Conclusions. The c.1578del variant, previously not described in literature in a homozygous state, causes Werner syndrome and is associated with the pronounced hallmarks of early senescence in the proband’s fibroblasts. Molecular diagnosis brings better treatment of manifestations and monitoring options for the patients, helping to establish more sufficient and secure patient care.
Diego López. Las Sátiras de Persio. Texto latino y traducción extraídos de su Declaración magistral
Authors: Del Amo Lozano Milagros 
Published: February 2025

Diego López, humanista español que tradujo y comentó a Virgilio (1601), Alciato (1615), Valerio Máximo (1631), y Juvenal (1642), realizó unos comentarios a las Sátiras de Persio que se publicaron en Burgos en 1609: Aulo Persio Flacco, traduzido en Lengua Castellana, por Diego Lopez, natural de la Villa de Valencia, Orden de Alcantara, y Preceptor en la ciudad de Toro. Con declaracion Magistral, en que se declaran todas las Historias, Fabulas, Antiguedades, versos dificultosos y moralidad que tiene el poeta. En esta publicación, Diego López ofrecía la primera traducción al español de la obra del satírico, mezclada con las abundantes explicaciones que, partiendo de Comentarios anteriores escritos en latín, aporta el autor al entendimiento de las Sátiras. Milagros del Amo ofrece exenta la traducción de Persio junto al texto latino que López traduce, lo cual ha logrado tras un análisis en profundidad del conjunto de la obra del humanista. La edición bilingüe (latín-español) que se ofrece va precedida de un pertinente y exhaustivo estudio.
Analisis Lokasi Upacara Ritual Budaya Dan Potensinya Untuk Pariwisata Di Kecamatan Jatinom Kabupaten Klaten
Authors: Panalensih Sekar Nafisah , Sarwono Sarwono , Ajar Seno Budhi 
Published: February 2025

<p>Penelitian ini bertujuan untuk (1) Mengetahui lokasi pusat upacara ritual budaya di Kecamatan Jatinom sebagai objek tujuan wisata. (2) Mengetahui dasar pemilihan lokasi pusat upacara ritual budaya di Kecamatan Jatinom sebagai objek tujuan wisata. (3) Mengetahui potensi pengembangan objek pariwisata di lokasi upacara ritual budaya sebagai objek tujuan wisata. Penelitian ini merupakan penelitian deskriptif kualitatif dengan pendekatan keruangan. Sumber data penelitian ini meliputi data primer yang didapat langsung dari lapangan dan data sekunder yang diperoleh dari instansi terkait. Teknik pengambilan sampel dilakukan dengan menggunakan <em>purposive sampling</em>. Pengumpulan data dilakukan dengan wawancara mendalam, observasi dan dokumentasi. Teknik uji validitas yang digunakan adalah triangulasi metode dari sumber data. Analisis data dengan menggunkan metode analisis kualitatif untuk mengetahui dasar pemilihan lokasi serta dengan cara Plotting dengan GPS untuk mengetahui sebaran objek wisata, menggunakan analisis potensi 4A yaitu Attraction, Accesibility, Amenity, dan Ancillary untuk mengetahui potensi dimasing-masing objek wisata.</p><p>Berdasarkan hasil penelitian dapat disimpulkan: (1) Lokasi pusat upacara ritual yang berada di Kematan Jatinom bervariasi. (2) Dasar pemilihan lokasi upacara ritual di Kecamatan Jatinom hampir semua sama yaitu Puitis Lokasi yang disucikan oleh manusia. (3) Dari 11 persebaran objek wisata yang ada di Kecamatan Jatinom memiliki 1 objek yang termasuk kedalam kelas sangat potensial, 5 objek yang termasuk kedalam kelas cukup potensial dan 5 objek termasuk kedalam kelas kurang potensial.</p><div class="WordSection1"><p>Kata kunci: Upacara Ritual, Persebaran, Potensi Wisata</p></div>
Pengembangan aksesibilitas antar pusat kegiatan pada kawasan perkotaan bagian utara Kabupaten Semarang
Authors: Farida Ani , Astuti Khristiana Dwi 
Published: February 2025

<div class="WordSection1"><p><span id="docs-internal-guid-9d486c2f-7fff-91f1-2196-f86e65d325c0"><span>Kabupaten Semarang terus berkembang seiring perannya sebagai penyangga Ibukota Provinsi Jawa Tengah serta bagian Kawasan Strategis Nasional Kedungsepur. Bagian utara yang bertampalan batas dengan Kota Semarang menjadi kawasan berkembang dengan tumbuhnya pusat-pusat kegiatan yang perlu didukung dengan arahan pengembangan aksesibilitas untuk menghubungkan antar pusat-pusat kegiatan yang ada. Penelitian ini bertujuan mengidentifikasi arah pengembangan aksesibilitas antar pusat kegiatan melalui pendekatan kuantitatif dengan analisis density dan kinerja jalan. Analisis density memberikan pertimbangan dalam penentuan pusat dan sub-pusat kegiatan serta analisis kinerja jalan yang meliputi perhitungan indeks aksesibilitas, indeks mobilitas, serta tingkat pelayanan jalan. Hasil penelitian menunjukkan bahwa kawasan terpadat yang menjadi pusat kegiatan sesuai dengan sistem pusat permukiman pada rencana struktur ruang di RTRW Kabupaten Semarang Tahun 2023-2043, dengan tiga titik pusat kegiatan yaitu PKL (Pusat Kegiatan Lokal) Perkotaan Ungaran serta PPK (Pusat Pelayanan Kawasan) Perkotaan Bergas dan Pringapus. Antara pusat kegiatan satu dengan pusat kegiatan lainnya sudah terhubung oleh jaringan jalan dengan nilai aksesibilitas dan mobilitas yang telah berada di atas standar pelayanan minimum pada kelas D dan F. Arahan pengembangan aksesibilitas pada tahun 2043 dilakukan melalui pelebaran badan jalan sesuai standar pada ruas Jalan Karangjati-Pringapus.</span></span></p></div>
Identifikasi faktor kegagalan perencanaan megaproyek: Studi kasus Megaproyek Brebes Internasional Industrial Park (Kawasan Industri Brebes)
Authors: Musyary Muhammad Daffa , Soehartono Annisya Alifvia 
Published: February 2025

<p dir="ltr"><span>Megaproyek Brebes Internasional Industrial Park (Kawasan Industri Brebes, KIB) merupakan salah satu proyek kawasan industri yang diamanahkan dalam Peraturan Presiden Nomor 79 tahun 2019 tentang Percepatan Pembangunan Ekonomi di Provinsi Jawa Tengah. Dengan luas 3.976 ha yang meliputi tiga kecamatan, yakni Kecamatan Bulakamba, Losari dan Tanjung, KIB akan menjadi kawasan industri terbesar se-Asia Tenggara. Namun, terdapat beberapa permasalahan yang menyebabkan pembangunan tersebut dialihkan ke Kabupaten Batang. Penelitian ini mengkaji kondisi pengelolaan megaproyek KIB dan mengidentifikasi faktor kegagalan dari perspektif pemangku kepentingan. Analisis deskriptif analitik dilakukan untuk memahami hubungan kondisi pengelolaan berbasis tinjauan literatur dan analisis </span><span>skoring</span><span> untuk menentukan faktor kegagalan mega proyek rencana KIB. Hasil penelitian menunjukkan bahwa faktor tantangan keuangan pada mega proyek Kawasan Industri Brebes merupakan faktor kegagalan utama. Salah satu permasalahan utama dalam pembiayaan adalah pembebasan lahan yang mengalami permasalahan karena banyak makelar tanah yang terlibat menyebabkan lonjakan harga lahan di Brebes melebihi Nilai Jual Objek Pajak. Hal tersebut menyebabkan biaya pembebasan lahan meningkat hingga melebihi anggaran awal sehingga target pembebasan lahan tidak tercapai hingga akhir tahun 2021.</span></p>
Preferensi pemilihan perumahan formal pada Desa Singopuran, Kabupaten Sukoharjo
Authors: Berlianty Selyna Aisya , Mukaromah Hakimatul , Kusumastuti Kusumastuti 
Published: February 2025

<span id="docs-internal-guid-0b88c01c-7fff-50af-907b-71326258ad5d"><span>Kota Surakarta memiliki mobilitas dan aktivitas yang tinggi sebagai Pusat Kegiatan Nasional (PKN) yang menimbulkan pergerakan kepada wilayah yang berbatasan dengan Kota Surakarta. Kabupaten Sukoharjo merupakan salah satu wilayah </span><span>urban fringe</span><span> dari Kota Surakarta akibat aktivitas dengan intensitas tinggi dan berdampak kepada wilayah Desa Singopuran. Perkembangan aktivitas juga menjadikan berkembangnya para penduduk yang menjadikan pertumbuhan dan kebutuhan tempat tinggal terus meningkat. Oleh karena itu, penelitian ini bertujuan untuk mengetahui preferensi pemilihan perumahan formal pada Desa Singopuran. Metode penelitian yang digunakan kuantitatif dengan penggunaan teknik analisis data yaitu analisis faktor dan analisis deskriptif. Hasil penelitian menunjukkan bahwa wilayah perumahan formal Desa Singopuran merupakan perumahan dengan kategori perumahan menengah. Faktor yang terbentuk terhadap preferensi para penduduk yaitu Faktor yang telah terbentuk antara lain faktor aksesibilitas fisik, legalitas, dan penghasilan; faktor harga bangunan dan sarana pendukung; faktor prasarana pendukung; faktor lingkungan kawasan perumahan; faktor desain bangunan dan lingkungan sosial; dan faktor karakteristik bangunan.</span></span>
Widespread Misstatements of Emissions by Major U.S. Corporations
Authors: Cohen Lauren , Rouen Ethan , Sachdeva Kunal 
Published: February 2025

Abstract

In the United States, corporations were directly responsible for more than 30 percent of the country’s 2022 total emissions, or approximately 2 billion metric tons of CO2 equivalents (1). Worried in part about the potential regulatory threats that companies face, investors and asset managers have called for greater transparency of publicly traded firms’ climate impacts. These calls have sparked a debate about whether government agencies should require such disclosures, or whether market pressures are sufficient to drive disclosure necessary for investors to make informed decisions (2). We show that these disclosures are, in fact, unreliable. Examining 900 emissions disclosures from 276 of the largest U.S. firms with available data, we find that 60% of these disclosures are eventually restated by the firm, a rate of restatement that has been consistent during the sample period. Furthermore, firms are more likely to understate than overstate, and the value of the understated emissions, on average, is more than twice the value of overstated emissions, suggesting that at least some of the misstatements are not errors. During the decade that we examine, the total gross understated public-firm emissions is 265 million tons, equivalent to the total emissions of Taiwan in 2020. Our results demonstrate the failure of market forces to produce reliable corporate environmental data.