bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspectives – Minimizing Observer Bias in Behavioral Studies: A Review and Recommendations
Authors: Burghardt Gordon M. , Bartmess‐LeVasseur Julia N. , Browning Sheri A. , Morrison Kathleen E. , Stec Courtney L. , Zachau Christopher E. , Freeberg Todd M. 
Published: May 2012

AbstractSince the 1970s, texts on research methods in animal behavior advocate that researchers minimize potential observer bias in their studies. One way to minimize possible bias is to record or score behavioral data blind to treatment, group, or individual. Another way to reduce bias is for researchers to analyze subsets or entire sets of data independently of one another and to obtain high inter‐observer reliability of behavioral coding. We reviewed several hundred published articles from 1970, 1980, 1990, 2000, and 2010 in five leading animal behavior journals and found that these two methods for minimizing or eliminating bias were rarely reported (<10% of articles reviewed). In contrast, a journal focusing on human infant behavior research was far more rigorous in incorporating methods to avoid bias (>80% of articles reviewed). The lack of reporting attempts to minimize bias in animal behavior studies suggests that, at best, many researchers view blind analyses of data or inter‐rater reliability as unimportant components of research or, if carried out, unnecessary to report in a manuscript. At worst, the lack of reporting attempts to minimize bias suggests that some published behavioral research may be unreliable. We are aware of constraints imposed by fieldwork and data collecting issues that make blind data comparisons or inter‐rater reliability assessments sometimes difficult or unfeasible. However, given that research ethicists often emphasize the fundamental importance of trust and transparency in science, we urge authors, reviewers, and editors of manuscripts to ensure that at least one of these two methods of reducing and reporting observer bias occurs.
Handstand Scent Marking in the Dwarf Mongoose (Helogale parvula)
Authors: Sharpe Lynda L. , Jooste Matthys M. , Cherry Michael I. 
Published: May 2012

AbstractMany mammal species adopt marking postures that elevate their scent deposits. The most extreme of these is handstand marking, in which an individual reverses against an upright object, flings its hind legs into the air above its back and balances bipedally on its fore feet. The resulting anogenital deposit is thus raised one full body length above ground level. It has been suggested that this energetically costly form of marking serves to provide conspecifics with information about the marker's body size and hence competitive ability. However, this explanation assumes that the height of an individuals’ deposit does reflect accurately its body size, an assumption that has never been tested in any hand‐standing species. This study investigated the relationship between body size and handstand mark height in a wild population of dwarf mongooses (Helogale parvula) in South Africa. We found that although body size and marking height were correlated positively for female dwarf mongooses, they were not related for males. Male dwarf mongooses (who are subject to intrasexual competition from outside their group) invested more heavily in anogenital range marking, marking at three times the female frequency and placing their deposits significantly higher than females (although they were not dimorphic). Males that were particularly vulnerable to rivals (i.e. those that were small for their age) tended to mark higher than more robust age‐mates, in keeping with the predictions of Adams & Mesterton‐Gibbons’ (1995, J. Theor. Biol.175, 405–421). model of deceptive threat communication. These findings suggest strongly that the height of anogenital scent deposits is of social significance to dwarf mongooses.
Regional variations in prevalence of postpartum depressive symptoms: Population‐based survey
Authors: Mishina Hiroki , Yamamoto Yosuke , Ito Masahiro 
Published: May 2012

AbstractThe purpose of the present study was to verify that there are regional variations in the detection rate of postpartum depressive symptoms in mothers. A population‐based survey of all women who gave birth in Fushimi Ward, Kyoto City during the period 1 December 2008–31 October 2010 was carried out. The Edinburgh Postnatal Depression Scale (EPDS) was completed by 2567 mothers of infants to measure postpartum depressive symptoms. The detection rate of postpartum depressive symptoms was compared between five localities. A significant difference was found between the lowest detection rate of postpartum depressive symptoms (8.4%) and the highest (18.1%; P < 0.001). A significant positive correlation was found between the proportion of houses in the locality receiving public assistance and the detection rate of postpartum depressive symptoms. The detection rate of postpartum depressive symptoms shows regional variations, suggesting a relationship with the poverty rate.
Insights into the complex influence of 5‐HT signaling on thalamocortical axonal system development
Authors: van Kleef Esmee S. B. , Gaspar Patricia , Bonnin Alexandre 
Published: May 2012

AbstractThe topographic organization of the thalamocortical axons (TCAs) in the barrel field (BF) in the rodent primary somatosensory cortex results from a succession of temporally and spatially precise developmental events. Prenatally, growth and guidance mechanisms enable TCAs to navigate through the forebrain and reach the cortex. Postnatally, TCAs grow into the cortex, and the refinement of their terminal arborization pattern in layer IV creates barrel‐like structures. The combined results of studies performed over the past 20 years clearly show that serotonin (5‐hydroxytryptamine; 5‐HT) signaling modulates these pre‐ and early postnatal developmental processes. In this context, 5‐HT signaling can purposely be described as ‘modulating’ rather than ‘controlling’ because developmental alterations of 5‐HT synthesis, uptake or degradation either have a dramatic, moderate or no effect at all on TCA pathway and BF formation. In this review we summarize and compare the outcomes of diverse pharmacological and genetic manipulations of 5‐HT signaling on TCA pathway and BF formation, in an attempt to understand these discrepancies.
Cryptic diversity in disjunct populations of Middle American Montane Pitvipers: a systematic reassessment of Cerrophidion godmani
Authors: Jadin Robert C. , Townsend Josiah H. , Castoe Todd A. , Campbell Jonathan A. 
Published: May 2012

Jadin, R.C., Townsend, J.H., Castoe, T.A. & Campbell, J.A. (2012). Cryptic diversity in disjunct populations of Middle American Montane Pitvipers: a systematic reassessment of Cerrophidion godmani. —Zoologica Scripta, 41, 455–470.The discovery and taxonomic recognition of cryptic species has become increasingly frequent with the application of molecular phylogenetic analyses, particularly for species with broad geographic distributions. In this study we focus on the venomous pitviper species Cerrophidion godmani that is widely distributed throughout the highlands of Central America. We provide evidence based on both molecular phylogenetic analyses and morphological data that C. godmani represents three deeply divergent lineages and is possibly non‐monophyletic. These three lineages are relatively conserved in their morphology and tend to be highly variable among individuals, but we do find sufficient morphological characters to diagnose them as evolutionarily distinct. We apply these data, together with known geographic distributions of populations, to infer boundaries of these three divergent evolutionary lineages. Based on the body of evidence, we formally name and describe two new species of Cerrophidion and redescribe C. godmani sensu stricto.
Substance use initiation: The role of simultaneous polysubstance use
Authors: OLTHUIS JANINE V. , DARREDEAU CHRISTINE , BARRETT SEAN P. 
Published: May 2012

AbstractIntroduction and Aims. Simultaneous polysubstance use (SPU) is a common phenomenon, yet little is known about its role in substance use initiation.Design and Methods. In the present study, 226 cannabis users completed structured interviews about their substance use history. For each substance ever used, participants provided details of their age of first use, their use in the preceding 30 days and whether they co‐administered any other licit or illicit substances the first time they used the substance.Results. For most illicit substances [powder cocaine, crack, amphetamine, methamphetamine, 3,4‐methylenedioxymethamphetamine (MDMA; ecstasy), heroin, opium, gamma‐hydroxybutyric acid (GHB), ketamine, psilocybin (magic mushrooms), mescaline, phencyclidine (PCP), peyote and inhalants], results showed that a clear majority of participants (≥75%) reported SPU during their first‐ever use of the substance. While SPU was less common on occasions of first use of alcohol, tobacco and cannabis, a high proportion of SPU on occasions of first use of ‘harder’ drugs could be accounted for by the co‐use of alcohol, tobacco and/or cannabis.Discussion and Conclusions. Such findings raise the possibility that specific alcohol, tobacco and/or cannabis use episodes might directly contribute to the initiation of new substance use. Understanding the role of SPU on occasions of first use might help better identify risk factors for substance use progression and improve intervention efforts.[Olthuis JV, Darredeau C, Barrett SP. Substance use initiation: The role of simultaneous polysubstance use. Drug Alcohol Rev 2013;32:67–71]
Earnings Variability and Disclosure of R&D: Evidence from Press Releases
Authors: Weiss Dan , Falk Haim , Zion Uri Ben 
Published: May 2012

AbstractThis study explores press releases in the pharmaceutical industry to expand our understanding of how investments in R&D outlays influence uncertainty of future earnings. The findings make two contributions to the literature. First, they provide evidence that equal investments in different R&D ventures are associated with differential variability of future earnings. This result suggests that non‐financial information contained in press releases captures attributes of firm‐specific R&D investments that are not revealed through R&D expenditures reported in financial statements. Second, prior studies have indicated that investments in pharmaceutical R&D are associated with the highest variability of future earnings among all industries. The results, however, suggest that for a large class of low‐risk pharmaceutical R&D investments, the relative variability of future earnings is low and similar to that generated by capital expenditures. The findings hold when we control for endogeneity in voluntary disclosure of press releases.
A multiparent advanced generation inter‐cross population for genetic analysis in wheat
Authors: Huang Bevan E. , George Andrew W. , Forrest Kerrie L. , Kilian Andrzej , Hayden Matthew J. , Morell Matthew K. , Cavanagh Colin R. 
Published: May 2012

SummaryWe present the first results from a novel multiparent advanced generation inter‐cross (MAGIC) population derived from four elite wheat cultivars. The large size of this MAGIC population (1579 progeny), its diverse genetic composition and high levels of recombination all contribute to its value as a genetic resource. Applications of this resource include interrogation of the wheat genome and the analysis of gene–trait association in agronomically important wheat phenotypes. Here, we report the utilization of a MAGIC population for the first time for linkage map construction. We have constructed a linkage map with 1162 DArT, single nucleotide polymorphism and simple sequence repeat markers distributed across all 21 chromosomes. We benchmark this map against a high‐density DArT consensus map created by integrating more than 100 biparental populations. The linkage map forms the basis for further exploration of the genetic architecture within the population, including characterization of linkage disequilibrium, founder contribution and inclusion of an alien introgression into the genetic map. Finally, we demonstrate the application of the resource for quantitative trait loci mapping using the complex traits plant height and hectolitre weight as a proof of principle.
If You Don't Count, You Don't Count: Monitoring and Evaluation in South African NGOs
Authors: Mueller‐Hirth Natascha 
Published: May 2012

ABSTRACT Non‐Governmental Organizations (NGOs) are increasingly challenged to demonstrate accountability and relevance, with reporting, monitoring and evaluation arguably having become development activities in their own right. Drawing on interviews and observation research, this article examines the impact of intensified monitoring and evaluation (M&E) requirements on a number of South African NGOs. M&E — and the types of expertise, vocabularies and practices it gives rise to — is an important area that is usually neglected in the study of NGOs but that significantly impacts on NGOs’ logic of operation. By focusing on three areas — data that are considered appropriate to conduct M&E, staffing and organizational cultures, and NGOs’ reformist relationships with other civil society organizations (CSOs) — M&E is revealed as a central discursive element in the constitution of NGOs appropriate to neoliberal development. By engaging a neo‐Foucauldian framework of governmentality, M&E practices are thus understood as technologies through which governing is accomplished in the trans‐scalar post‐apartheid development domain.
Democratic Assertions: The Making of India's Recognition of Forest Rights Act
Authors: Kumar Kundan , Kerr John M. 
Published: May 2012

ABSTRACT Inclusion of marginalized sections and minorities remains one of the most vexing problems for democratic politics. This article discusses the enactment of a recent Indian law, ‘The Scheduled Tribes and Other Traditional Forest Dwellers (Recognition of Rights) Act, 2006’, as exemplifying the possibilities of inclusion of marginalized groups in democratic processes. The law was enacted in response to a nationwide mobilization of marginalized forest dwellers and their advocates demanding rights over forests. Grassroots‐level formations representing forest dwellers came together across scales and spaces to form a network that successfully negotiated India's democratic politics to achieve the passage of the law. The case illustrates the role of grassroots mobilizations in creating alternate discourses of legitimacy, networking across scales and locations, and using spaces provided by representative democracy to include the voices and demands of the marginalized in democracies.
Audit Bodies: Embodied Participation, Disability Universalism, and Accessibility in India
Authors: Friedner Michele , Osborne Jamie 
Published: May 2012

Abstract:  In this article we think critically about the role of the “access audit” in creating new forms of embodied participation, experiential and technical expertise, and imaginaries of what the modern Indian city should be. We analyze how disability activists make claims about the relationship between subjective bodily experiences and bodies of objective knowledge. We also explore the emergence of a professional access audit apparatus focused on technical standards. As neither volunteer nor professional access audits result in significant architectural or structural changes, we are interested in what other effects and affects these audits produce and what discursive authority claims of inaccessibility have.This article analyzes the practice of conducting “access audits” by lay and professional disability rights activists and organizations in urban India. We argue that access audits are overly focused and that they have limited impact. In overly focusing on physical and technical access, auditors miss the importance of programmatic and policy interventions as well as the need for a more collective contentious politics. Our research illustrates that the contested field of access auditing appears to prevent a unified disability coalition from forming. In addition, it is important for auditors to think critically about the concept of “access” and ask what social, economic, and political processes are embedded within the concept.
Factors other than genotype account largely for the phenotypic variation of the pulmonary valve in Syrian hamsters
Authors: Carmen Fernández M. , Durán Ana C. , Fernández Borja , Arqué Josep M. , Anderson Robert H. , Sans‐Coma Valentín 
Published: May 2012

AbstractUnderstanding of the aetiology of congenitally anomalous pulmonary valves remains incomplete. The aim of our study, therefore, was to elucidate the degree to which the phenotypic variation known to exist for the pulmonary valve relies on genotypic variation. Initially, we tested the hypothesis that genetically alike individuals would display similar valvar phenotypes if the phenotypic arrangement depended entirely, or almost entirely, on the genotype. Thus, we examined pulmonary valves from 982 Syrian hamsters belonging to two families subject to systematic inbreeding by crossing siblings. Their coefficient of inbreeding was 0.999 or higher, so they could be considered genetically alike. External environmental factors were standardized as much as possible. A further 97 Syrian hamsters from an outbred colony were used for comparative purposes. In both the inbred and outbred hamsters, we found valves with a purely trifoliate, or tricuspid, design, trifoliate valves with a more or less extensive fusion of the right and left leaflets, bifoliate, or bicuspid, valves with fused right and left leaflets, with or without a raphe located in the conjoined arterial sinus, and quadrifoliate, or quadricuspid, valves. The incidence of the different valvar morphological variants was similar in the outbred and inbred colonies, except for the bifoliate pulmonary valves, which were significantly more frequent in the hamsters from one of the two inbred families. Results of crosses between genetically alike hamsters revealed no significant association between the pulmonary valvar phenotypes as seen in the parents and their offspring. The incidence of bifoliate pulmonary valves, nonetheless, was higher than statistically expected in the offspring of crosses where at least one of the parents possessed a pulmonary valve with two leaflets. Our observations are consistent with the notion that the basic design of the pulmonary valve, in terms of whether it possesses three or two leaflets, relies on genotypic determinants. They also denote that the bifoliate condition of the valve is the consequence of complex inheritance, with reduced penetrance and variable expressivity. Moreover, in showing that the incidence of the bifoliate pulmonary valve significantly differs in two different isogenetic backgrounds, our data suggest that genetic modifiers might be implicated in directing the manifestation of such specific pulmonary valvar malformations. Finally, our findings indicate that factors other than the genotype, operating during embryonic life and creating developmental noise, or random variation, play a crucial role in the overall phenotypic variation involving the pulmonary valve.
Chromatographic separation of reaction products from the choline acetyltransferase and carnitine acetyltransferase assay: differential ChAT and CrAT activity in brain extracts from Alzheimer’s disease versus controls
Authors: Bailey Jason A. , Lahiri Debomoy K. 
Published: May 2012

J. Neurochem. (2012) 122, 672–680.AbstractCholine acetyltransferase (ChAT) catalyzes the reaction between choline and acetylcoenzyme A (AcCoA) to form acetylcholine (ACh) in nerve terminals. ACh metabolism has implications in numerous aspects of physiology and varied disease states, such as Alzheimer’s disease. Therefore a specific, sensitive, and reliable method for detecting ChAT enzyme activity is of great utility in a number of situations. Using an existing radionuclide‐based enzyme activity assay, we have observed detectable ChAT signals from non‐cholinergic cells, suggesting a contaminant in the assay producing an artifactual signal. Previous reports have suggested that L‐acetylcarnitine (LAC) contaminates many assays of ChAT activity, because of difficulties in separating LAC from ACh by organic extraction. To determine the source of this hypothesized artifact and to rectify the problem, we have developed a paper chromatography‐based assay for the detection of acetylcholine and other contaminating reaction products of this assay, including LAC. Our first goal was to develop a simple and economical method for resolving and verifying the identities of various reaction products or contaminants that could be performed in most laboratories without specialized equipment. Our second goal was to apply this separation method in postmortem human brain tissue samples. Our assay successfully detected several contaminants, especially in assays using brain tissue, and allowed the separation of the intended ACh product from these contaminants. We further demonstrate that this assay can be used to measure carnitine acetyltransferase (CrAT) activity in the same samples, and assays comparing ChAT and CrAT show that CrAT is highly active in neuronal tissues and in neuronal cell cultures relative to ChAT. Thus, the simple chromatography‐based assay we describe allows the measurement of specific reaction products separated from contaminants using commonly available and inexpensive materials. Further, we show that ChAT activity is significantly reduced in brain extracts from Alzheimer’s disease compared to controls.
Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate
Authors: Khedr Naglaa F. , El‐Ashmawy Nahla E. , El‐Bahrawy Hoda A. , Haggag Ali A. , El‐Abd Eman E. 
Published: May 2012

AbstractOsteoporosis is a reduction in bone mineral density (BMD). It develops less often in men than in women. This study aimed to evaluate the bone protective effects of raloxifene (RAL), risedronate (RIS), and their combination on osteoporotic male rats. Forty male Wister rats (12 weeks) were randomly divided into five groups: sham‐operated group (n = 8), orchidectomized (ORX) group (n = 7), RAL group (n = 9), RIS group (n = 7) and RAL + RIS group (n = 7). RAL was orally administered at 3 mg/kg three times/week, and RIS was given subcutaneously at 5 μg/kg, twice weekly. After 6 weeks of treatment, serum cathepsin‐K, alkaline (ALP) and acid phosphatase activities, serum osteocalcin, serum Ca2+, and Pi were determined. Urinary Ca2+ and deoxypyridinoline levels, BMD, and Ca2+ content of femur ash were estimated. Histochemical localization of ALP activity of tibia and histomorphometry was examined. As compared to sham, ORX rats showed a significant increase in bone turnover markers, and histochemical activity of ALP was increased markedly in proximal tibia of ORX rats, whereas BMD and Ca2+ content of femur ash were reduced after ORX. These changes were modulated after treatment with RAL and RIS or both to ORX rats; BMD of femur was improved by each treatment, and bone turnover markers were reduced as compared to ORX vehicle group. We concluded that orchidectomy induced osteoporosis and increased bone turnover in male rats because of withdrawal of sex hormones. Both RAL and RIS could treat osteoporosis in ORX rats; they reduced bone turnover markers and maintained BMD.