bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel technique of chronic total occlusion retrograde wire crossing by wiring into the antegrade microcatheter
Authors: Liu Wei , Wagatsuma Kenji 
Published: March 2010

AbstractWe described a novel transradial retrograde wiring technique to treat chronic total occlusion (CTO) of the left anterior descending artery. When both retrograde wire and kissing wire technique failed to cross the CTO lesion, an antegrade microcatheter was placed in the CTO lesion. Then, a retrograde wire was manipulated into the antegrade microcatheter and subsequently exchanged and wire externalized. This technique appears to be feasible and safe alternative for retrograde wire crossing of the CTO lesion. It is a less traumatic modified version of controlled antegrade and retrograde subintimal tracking (CART) technique that can be attempted before embarking on CART or reverse CART strategy. © 2010 Wiley‐Liss, Inc.
Efficacy and safety of bivalirudin in patients receiving clopidogrel therapy after diagnostic angiography for percutaneous coronary intervention in acute coronary syndromes
Authors: Feldman Dmitriy N. , Minutello Robert M. , Bergman Geoffrey , Moussa Issam , Wong S. Chiu 
Published: March 2010

AbstractObjectives: This study sought to investigate if the efficacy of bivalirudin monotherapy is similar to heparin plus GP IIb/IIIa inhibition in patients with acute coronary syndromes (ACS) treated with clopidogrel following diagnostic angiography. Background: Prior trials have demonstrated that peri‐procedural bivalirudin therapy confers similar efficacy as heparin plus GP IIb/IIIa inhibitors, while lowering the risk of bleeding complications in ACS patients undergoing percutaneous coronary intervnetions (PCI). However, the incidence of adverse ischemic events post‐PCI appeared to be higher in patients receiving bivalirudin without adequate pretreatment with clopidogrel. Methods: Using the 2004/2005 Cornell Angioplasty Registry, we evaluated 980 consecutive patients undergoing urgent PCI for UA/NSTEMI who were treated with either bivalirudin or UFH plus GP IIb/IIIa inhibitor. We excluded patients who were on chronic clopidogrel therapy or received clopidogrel pretreatment prior to angiography. All patients received a clopidogrel load (≥300‐mg dose) immediately before or after the PCI. Long‐term all‐cause mortality was obtained for 100% of patients, with a mean follow‐up of 24.6 ± 7.7 months. Results: Of the 980 study patients, 461 (47.0%) were treated with bivalirudin and 519 (53.0%) patients received UFH plus GP IIb/IIIa inhibitor. DES were used in 88% of PCI; 45% of patients presented with NSTEMI. The incidence of in‐hospital death (0.4% vs. 0.2%, P = 0.604), post‐procedural MI (6.9% vs. 5.4%, P = 0.351), and MACE including death, stroke, emergent CABG/PCI, and MI (7.6% vs. 5.8%, P = 0.304) were similar in patients treated with bivalirudin versus UFH plus GP IIb/IIIa inhibitors, respectively. The incidence of in‐hospital stent thrombosis was similar (0.7% vs. 0%, P = 0.104), while major (0.9% vs. 2.9%, P = 0.034) and minor bleeding (10.4% vs. 18.9%, P < 0.001) was reduced in the bivalirudin‐treated group. By two‐years of follow‐up, after propensity‐score adjusted multivariate Cox regression analysis, there was no significant difference in long‐term mortality between the two groups (HR 1.18; 95%CI 0.64–2.19, P = 0.598). Conclusions: In patients presenting with ACS and receiving clopidogrel treatment after angiography (before or within 30 min of PCI), peri‐procedural bivalirudin monotherapy suppresses acute and long‐term adverse events to a similar extent as does UFH plus GP IIb/IIIa inhibitors, while significantly lowering the risk of bleeding complications. © 2010 Wiley‐Liss, Inc.
Percutaneous treatment of iatrogenic left‐anterior descending artery to right ventricle fistula
Authors: Oreglia Jacopo A. , Bruschi Giuseppe , Klugmann Silvio 
Published: March 2010

AbstractIatrogenic coronary fistula is a rare condition whose treatment strategy is not well established. We report the case of a 70‐year‐old patient who came to our attention with echocardiographic and scintigraphic signs of myocardial ischemia and who had undergone heart transplantation 10 years earlier. The coronary angiogram showed a coronary fistula connecting the left‐anterior descending artery to the right ventricle via an enlarged septal branch. Because of the presence of myocardial ischemia, the patient was scheduled for percutaneous coronary intervention. The fistula was sealed with implant of two polytetrafluoroethylene (PTFE)‐covered stents that excluded the septal branch. One‐year follow‐up showed persistence of the good result in terms of angiographic and clinical outcome. © 2010 Wiley‐Liss, Inc.
Increasing the Sensitivity and Single‐Base Mismatch Selectivity of the Molecular Beacon Using Graphene Oxide as the “Nanoquencher”
Authors: Lu Chun‐Hua , Li Juan , Liu Jing‐Jing , Yang Huang‐Hao , Chen Xi , Chen Guo‐Nan 
Published: March 2010

AbstractHere, we report a novel, highly sensitive, selective and economical molecular beacon using graphene oxide as the “nanoquencher”. This novel molecular beacon system contains a hairpin‐structured fluorophore‐labeled oligonucleotide and a graphene oxide sheet. The strong interaction between hairpin‐structured oligonucleotide and graphene oxide keep them in close proximity, facilitating the fluorescence quenching of the fluorophore by graphene oxide. In the presence of a complementary target DNA, the binding between hairpin‐structured oligonucleotide and target DNA will disturb the interaction between hairpin‐structured oligonucleotide and graphene oxide, and release the oligonucleotide from graphene oxide, resulting in restoration of fluorophore fluorescence. In the present study, we show that this novel graphene oxide quenched molecular beacon can be used to detect target DNA with higher sensitivity and single‐base mismatch selectivity compared to the conventional molecular beacon.
From a Network of Computed Reaction Enthalpies to Atom‐Based Thermochemistry (NEAT)
Authors: Császár Attila G. , Furtenbacher Tibor 
Published: March 2010

AbstractA simple and fast, weighted, linear least‐squares refinement protocol and code is presented for inverting the information contained in a network of quantum chemically computed 0 K reaction enthalpies. This inversion yields internally consistent 0 K enthalpies of formation for the species of the network. The refinement takes advantage of the fact that the accuracy of computed enthalpies depends strongly on the quantum‐chemical protocol employed for their determination. Different protocols suffer from different sources of error; thus, the reaction enthalpies computed by them have “random” residual errors. Since it is much more natural for quantum‐chemical energy and enthalpy results, including reaction enthalpies, to be based on the electronic ground states of the atoms and not on the historically preferred elemental states, and since these two possible protocols can be converted into each other straightforwardly, it is proposed that first‐principles thermochemistry should employ the ground electronic states of atoms. In this scheme, called atom‐based thermochemistry (AT), the enthalpy of formation of a gaseous compound corresponds simply to the total atomization energy of the species; it is always positive, and it reflects the bonding strength within the molecule. The inversion protocol developed and based on AT is termed NEAT, which represents the fact that the protocol proceeds from a network of computed reaction enthalpies toward atom‐based thermochemistry, most directly to atom‐based enthalpies of formation. After assembling a database that consisted of 361 ab initio reactions and reaction enthalpies involving 188 species, collected from 31 literature sources, the following dependable 0 K atom‐based enthalpies of formation, Δf${H{{{\rm AT}\hfill \atop 0\hfill}}}$, all in kJ mol−1, have been obtained by means of NEAT: H2=432.07(0), CH=334.61(15), NH=327.69(25), OH=425.93(21), HF=566.13(31), CO=1072.08(28), O2=493.51(34), CH2=752.40(21), H2O=918.05(20), HO2=694.53(32), CO2=1597.77(40), CH3=1209.64(29), NH3=1157.44(33), C2H2=1625.78(40), and CH4=1641.68(40), in which the uncertainty values given in parentheses represent 95 % confidence intervals. The average deviation of these values from the well‐established active thermochemical tables (ATcT) values is a mere 0.25 kJ mol−1, with a maximum deviation of 0.7 kJ mol−1. This shows that the use of a large number of ab initio reaction enthalpies within a NEAT‐type protocol has considerable advantages over the sequential utilization of the ab initio information.
New Fluorinated Rhodamines for Optical Microscopy and Nanoscopy
Authors: Mitronova Gyuzel Yu. , Belov Vladimir N. , Bossi Mariano L. , Wurm Christian A. , Meyer Lars , Medda Rebecca , Moneron Gael , Bretschneider Stefan , Eggeling Christian , Jakobs Stefan , Hell Stefan W. 
Published: March 2010

AbstractNew photostable rhodamine dyes represented by the compounds 1 a–r and 3–5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N′,N‐bis(2,2,2‐trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3‐H and 5 were found to undergo sulfonation of the xanthene fragment at the positions 4′ and 5′. Two fluorine atoms were introduced into the positions 2′ and 7′ of the 3′,6′‐diaminoxanthene fragment in compounds 1 a–d, 1 i–l and 1 m–r. The new rhodamine dyes may be excited with λ=488 or 514 nm light; most of them emit light at λ=512–554 nm (compounds 1 q and 1r at λ=576 and 589 nm in methanol, respectively) and have high fluorescence quantum yields in solution (up to 98 %), relatively long excited‐state lifetimes (>3 ns) and are resistant against photobleaching, especially at high laser intensities, as is usually applied in confocal microscopy. Sulfonation of the xanthene fragment with 30 % SO3 in H2SO4 is compatible with the secondary amide bond (rhodamine‐CON(Me)CH2CH2COOH) formed with MeNHCH2CH2COOCH3 to providing the sterically unhindered carboxylic group required for further (bio)conjugation reactions. After creating the amino reactive sites, the modified derivatives may be used as fluorescent markers and labels for (bio)molecules in optical microscopy and nanoscopy with very‐high light intensities. Further, the new rhodamine dyes are able to pass the plasma membrane of living cells, introducing them as potential labels for recent live‐cell‐tag approaches. We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments.
Phthalocyanine–Polyamine Conjugates as pH‐Controlled Photosensitizers for Photodynamic Therapy
Authors: Jiang Xiong‐Jie , Lo Pui‐Chi , Tsang Yee‐Man , Yeung Sin‐Lui , Fong Wing‐Ping , Ng Dennis K. P. 
Published: March 2010

AbstractA series of aryl hydroxyamines prepared by reductive amination were treated with silicon(IV) phthalocyanine dichloride in the presence of pyridine to give the diaxially substituted phthalocyanine–polyamine conjugates 1–5. The electronic absorption, fluorescence emission, and efficiency at generating reactive oxygen species of these compounds were all sensitive to the pH environment. Under acidic conditions, the fluorescence quantum yields and the singlet oxygen quantum yields of these compounds were greatly enhanced in DMF as a result of protonation of the amino moieties, which inhibited the photoinduced electron‐transfer deactivation pathway. The Q band was diminished and broadened, and the fluorescence intensity decreased as the pH increased in citrate buffer solutions. The rate of superoxide radical formation was also reduced in a higher pH environment. Compound 3, containing a terminal 4‐chlorophenyl group at the axial substituent, showed the most desirable pH‐responsive properties, which makes it a promising tumor‐selective fluorescence probe and photosensitizer for photodynamic therapy. All of the phthalocyanines 1–5 were highly photocytotoxic against HT29 and HepG2 cells with IC50 values as low as 0.03 μM. Compound 3 was highly selective toward lysosomes, but not mitochondria of HT29 cells.
Applying Neural Network Classification to Obtain Mangrove Landscape Characteristics for Monitoring the Travel Environment Quality on the Beihai Coast of Guangxi, P. R. China
Authors: Yu Xiang , Shao Hong‐bo , Liu Xiang‐hua , Zhao Dong‐zhi 
Published: March 2010

AbstractThe spectral characteristics of mangroves on the Beihai Coast of Guangxi, P. R. China are acquired on the basis of spectral data from field measurements. Following this, the 3‐layer reverse‐conversing neural networks (NN) classification technology is used to analyze the Landsat TM5 image obtained on January 8, 2003. It is detailed enough to facilitate the introduction of the algorithm principle and trains project of the neural network. Neural network algorithms have characteristics including large‐scale data handling and distributing information storage. This research firstly analyzes the necessity and complexity of this translation system, and then introduces the strong points of the neural network. Processing mangrove landscape characteristics by using neural network is an important innovation, with great theoretical and practical significance. This kind of neural network can greatly improve the classification accuracy. The spatial resolution of Landsat TM5 is high enough to facilitate the research, and the false color composite from 3‐, 4‐, and 5‐bands has a clear boundary and provides a significant quantity of information and effective images. On the basis of a field survey, the exported layers are defined as mangrove, vegetation, bare land, wetlands and shrimp pool. TM satellite images are applied to false color composites by using 3‐, 4‐, and 5‐bands, and then a supervised classification model is used to classify the image. The processing method of hyper‐spectrum remote sensing allows the spectral characteristics of the mangrove to be determined, and integrates the result with the NN classification for the false color composite by using 3‐, 4‐, and 5‐bands. The network model consists of three layers, i. e., the input layer, the hidden layer, and the output layer. The input layer number of classification is defined as 3, and the hidden layers are defined as 5 according to the function operation. The control threshold is 0.9. The training ratio is 0.2. The maximum permit error is 0.08. The classification precision reaches 86.86%. This is higher than the precision of maximal parallel classification (50.79%) and the spectrum angle classification (75.39%). The results include the uniformity ratio (1.7789), the assembly ratio (0.6854), the dominance ratio (–1.5850), and the fragmentation ratio (0.0325).
Cloud Point Extraction for the Preconcentration of Silver and Palladium in Real Samples and Determination by Atomic Absorption Spectrometry
Authors: Tavallali Hossein , Yazdandoust Saeed , Yazdandoust Mozhdeh 
Published: March 2010

AbstractA cloud point extraction procedure is presented for the preconcentration and simultaneous determination of Ag+ and Pd2+ in various samples. After complexation with 2‐((2‐((1H‐benzo[d]imidazole‐2‐yl)methoxy)phenoxy)methyl)‐1H‐benzo[d]imidazol (BIMPI), which was used as a new chelating agent, analyte ions were quantitatively extracted to a phase rich in Triton X‐114 following centrifugation, and determination was carried out by flame atomic absorption spectrometry (FAAS). Under the optimum experimental conditions (i. e., pH = 7.0, 15.0·10–5 mol/L BIMPI and 0.036% (w/v) Triton X‐114), calibration graphs were linear in the range of 28.0–430.0 μg/L and 57.0–720.0 μg/L with detection limits of 10.0 and 25.0 μg/L for Ag+ and Pd2+, respectively. The enrichment factors were 35.0 and 28.0 for Ag+ and Pd2+, respectively. The method has been successfully applied to evaluate these metals in some real samples, including waste water, soil and hydrogenation catalyst samples.
Removal of NO3– from Drinking Water by Electrocoagulation – An Alternate Approach
Authors: Vasudevan Subramanyan , Epron Florence , Lakshmi Jothinathan , Ravichandran Subbiah , Mohan Swaminathan , Sozhan Ganapathy 
Published: March 2010

AbstractThe present study provides an electrocoagulation method, for the removal of NO3– from drinking water using magnesium as the anode and cathode. The experiments are carried out as a function of pH, temperature, and current density. The results show that the maximum removal efficiency of 95.8% was achieved at a current density of 0.25 A/dm2, at a pH of 7.0. The adsorption of NO3– preferably fitting the Langmuir adsorption isotherm suggests monolayer coverage of the adsorbed molecules. The adsorption process follows a second‐order kinetics model. Thermodynamic studies show that the adsorption was exothermic and spontaneous in nature.
Detection of Pharmaceuticals and Personal Care Products in Agricultural Soils Receiving Biosolids Application
Authors: Wu Chenxi , Spongberg Alison L. , Witter Jason D. , Fang Min , Ames April , Czajkowski Kevin P. 
Published: March 2010

AbstractIncreasing research has suggested that biosolids generated from municipal wastewater treatment can be a major sink for many pharmaceuticals and personal care products (PPCPs) and their land application potentially introduces these contaminants into the terrestrial and aquatic environments. In this study, methods were developed for the analysis of 14 PPCPs in biosolids and soils using pressurized liquid extraction, solid phase extraction and liquid chromatography‐tandem mass spectrometry. Recoveries were over 50% for all analytes except diphenhydramine (˜30%) in soils. Soil properties or type of biosolids showed minor effects on method recoveries. Estimated method limits of quantification (LOQ) range from 0.1–15 ng g–1 for soil and 0.3–27 ng g–1 for biosolids. A field study utilizing the methods revealed that other than carbamazepine‐10,11‐epoxide, all targeted compounds were detected in biosolids. Diphenhydramine, fluoxetine, triclosan and triclocarban were detected up to the μg g–1 range with the highest concentration of 23 μg g–1 for triclocarban. Seven of the PCCPs found in biosolids were also detected in agricultural soils amended with these biosolids and several (carbamazepine, diphenhydramine, and triclocarban) appeared to be persistent in soils. Triclocarban was also found most abundant in soils with the highest average concentration of 0.2 μg g–1 while the rest of compounds were in the lower ng g–1 range. Generally, the concentrations found on the fields were 2–3 degrees of magnitude lower than in the biosolids, which is likely to be due to dilution, degradation and leaching processes.
Novel interactors and a role for supervillin in early cytokinesis
Authors: Smith Tara C. , Fang Zhiyou , Luna Elizabeth J. 
Published: March 2010

AbstractSupervillin, the largest member of the villin/gelsolin/flightless family, is a peripheral membrane protein that regulates each step of cell motility, including cell spreading. Most known interactors bind within its amino (N)‐terminus. We show here that the supervillin carboxy (C)‐terminus can be modeled as supervillin‐specific loops extending from gelsolin‐like repeats plus a villin‐like headpiece. We have identified 27 new candidate interactors from yeast two‐hybrid screens. The interacting sequences from 12 of these proteins (BUB1, EPLIN/LIMA1, FLNA, HAX1, KIF14, KIFC3, MIF4GD/SLIP1, ODF2/Cenexin, RHAMM, STARD9/KIF16A, Tks5/SH3PXD2A, TNFAIP1) co‐localize with and mis‐localize EGFP‐supervillin in mammalian cells, suggesting associations in vivo. Supervillin‐interacting sequences within BUB1, FLNA, HAX1, and MIF4GD also mimic supervillin over‐expression by inhibiting cell spreading. Most new interactors have known roles in supervillin‐associated processes, e.g. cell motility, membrane trafficking, ERK signaling, and matrix invasion; three (KIF14, KIFC3, STARD9/KIF16A) have kinesin motor domains; and five (EPLIN, KIF14, BUB1, ODF2/cenexin, RHAMM) are important for cell division. GST fusions of the supervillin G2‐G3 or G4‐G6 repeats co‐sediment KIF14 and EPLIN, respectively, consistent with a direct association. Supervillin depletion leads to increased numbers of bi‐ and multi‐nucleated cells. Cytokinesis failure occurs predominately during early cytokinesis. Supervillin localizes with endogenous myosin II and EPLIN in the cleavage furrow, and overlaps with the oncogenic kinesin, KIF14, at the midbody. We conclude that supervillin, like its interactors, is important for efficient cytokinesis. Our results also suggest that supervillin and its interaction partners coordinate actin and microtubule motor functions throughout the cell cycle. © 2010 Wiley‐Liss, Inc.
Bucket‐based authentication for outsourced databases
Authors: Wang Jieping , Du Xiaoyong , Lu Jiaheng , Lu Wei 
Published: March 2010

AbstractWhen outsourced database owners delegate their data to service providers, which might be untrusted or compromised, two issues of data security emerge, including data confidentiality and data integrity. Most of the previous research focuses on only one issue and the solution to integrate two approaches is expensive. In this paper, we propose bucket‐based authentication that can keep data confidentiality and meanwhile guarantee data integrity. Specifically, we first propose a new approach based on bucket checksum, which can be used for the authentication of multiple tuples at one time. We then apply bucket checksum to the authentication of various types of queries in static scenarios, including range queries and aggregation queries, such as MIN, MAX, SUM and COUNT queries. In the authentication of aggregation queries, several pruning rules have been proposed to improve performance further. We also extend our approach to dynamic scenarios based on incremental hash. Cost analysis shows the advantages of our approach over previous ones in terms of construction and verification cost. Experimental results show that our approach is both efficient and effective. Copyright © 2010 John Wiley & Sons, Ltd.
Site‐Specific DNA‐Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers
Authors: Simonsson Lisa , Jönsson Peter , Stengel Gudrun , Höök Fredrik 
Published: March 2010

AbstractWe investigate the Ca2+‐triggered fusion of lipid vesicles site‐selectively tethered to a DNA‐modified supported lipid bilayer array, with the DNA strands designed such that hybridization occurs in a zipperlike fashion. Prior to the addition of Ca2+, which is observed to induce docking and subsequent fusion (within 200 ms), the vesicles display lateral mobility determined by the number of tethers. Fusion is observed to require around ten DNA strands per vesicle, but does not occur at higher DNA coverage. However, despite the fact that fusion was restricted to occurring for vesicles tethered with around ten DNA strands, there is no correlation between single‐vesicle diffusivity and fusogenicity. A possible scenario for the DNA‐ induced fusion machinery, consistent with these observations, is that prior to Ca2+‐induced docking, the vesicles diffuse with a small number (2–4) of DNA tethers. Upon addition of Ca2+, the vesicles dock, presumably due to bridging of lipid head groups. Fusion then occurs under conditions where 10–16 DNA tethers form and rearrange at the rim of the contact region between a docked vesicle and the SLB. The time required for this rearrangement, which may include both DNA hybridization and dehybridization during zipping, is expected to represent the observed docking and fusion time of less than 200 ms.