bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occipital‐synarcual joint mobility in ratfishes (Chimaeridae) and its possible adaptive role
Authors: Romanov Alexey V. , Shakhparonov Vladimir V. , Gerasimov Kyrill B. , Korzun Leonid P. 
Published: June 2024

AbstractThe neurocranial elevation generated by axial muscles is widespread among aquatic gnathostomes. The mechanism has two functions: first, it contributes to the orientation of the mouth gape, and second, it is involved in suction feeding. To provide such mobility, anatomical specialization of the anterior part of the vertebral column has evolved in many fish species. In modern chimaeras, the anterior part of the vertebral column develops into the synarcual. Possible biological roles of the occipital‐synarcual joint have not been discussed before. Dissections of the head of two species of ratfishes (Chimaera monstrosa and Chimaera phantasma) confirmed the heterocoely of the articulation surface between the synarcual and the neurocranium, indicating the possibility of movements in the sagittal and frontal planes. Muscles capable of controlling the movements of the neurocranium were described. The m. epaxialis is capable of elevating the head, the m. coracomandibularis is capable of lowering it if the mandible is anchored by the adductor. Lateral flexion is performed by the m. lateroventralis, for which this function was proposed for the first time. The first description of the m. epaxialis profundus is given, its function is to be elucidated in the future. Manipulations with joint preparations revealed a pronounced amplitude of movement in the sagittal and frontal planes. Since chimaeras generate weak decrease in pressure in the oropharyngeal cavity when sucking in prey, we hypothesised the primary effect of neurocranial elevation, in addition to the evident lateral head mobility, is accurate prey targeting.
The dynamic molecular characteristics of neutrophils are associated with disease progression in dengue patients
Authors: Ou Guanyong , Liu Jiexiang , Zou Rongrong , Gu Yuchen , Niu Shiyu , Yin Juzhen , Yuan Jing , Qu Zhijun , Yang Yang , Liu Yingxia 
Published: June 2024

AbstractDengue, the most prevalent mosquito‐borne disease worldwide, poses a significant health burden. This study integrates clinical data and transcriptomic datasets from different phases of dengue to investigate distinctive and shared cellular and molecular features. Clinical data from 29 dengue patients were collected and analyzed alongside a public transcriptomic data set (GSE28405) to perform differential gene expression analysis, functional enrichment, immune landscape assessment, and development of machine learning model. Neutropenia was observed in 54.79% of dengue patients, particularly during the defervescence phase (65.79%) in clinical cohorts. Bioinformatics analyses corroborated a significant reduction in neutrophil immune infiltration in dengue patients. Receiver operating characteristic curve analysis demonstrated that dynamic changes in neutrophil infiltration levels could predict disease progression, especially during the defervescence phase, with the area under the curve of 0.96. Three neutrophil‐associated biomarkers—DHRS12, Transforming growth factor alpha, and ZDHHC19—were identified as promising for diagnosing and predicting dengue progression. In addition, the activation of neutrophil extracellular traps was significantly enhanced and linked to FcγR‐mediated signaling pathways and Toll‐like receptor signaling pathways. Neutrophil activation and depletion play a critical role in dengue's immune response. The identified biomarkers and their associated pathways offer potential for improved diagnosis and understanding of dengue pathogenesis and progression.
Guanylate‐binding protein 1 inhibits Hantaan virus infection by restricting virus entry
Authors: Gu Tianle , Qu Sirui , Zhang Junmei , Ying Qikang , Zhang Xiaoxiao , Lv Yunhua , Liu Rongrong , Feng Yunan , Wang Fang , Wu Xingan 
Published: June 2024

AbstractHantaan virus (HTNV) infection can cause hemorrhagic fever with renal syndrome (HFRS) in humans, and currently, there are no long‐standing protective vaccines or specific antivirals available. Guanylate‐binding protein 1 (GBP1) is an interferon‐stimulated gene that defends against various pathogen infections. However, the function of GBP1 in HTNV infection remains unknown. Here, we describe how GBP1 prevents HTNV infection by obstructing virus entry. We found that HTNV infection induced GBP1 expression and that overexpression of GBP1 inhibited HTNV infection, while knockout of GBP1 had the opposite effect. Interestingly, GBP1 did not affect interferon (IFN) signaling during HTNV infection. Instead, GBP1 prevented HTNV from entering cells through clathrin‐mediated endocytosis (CME). We also discovered that GBP1 specifically interacted with actin but not dynamin 2 (DNM2) and made it difficult for DNM2 to be recruited by actin, which may account for the suppression of CME during HTNV infection. These findings establish an antiviral role for GBP1 in inhibiting HTNV infection and help us better understand how GBP1 regulates HTNV entry and could potentially aid in developing treatments for this virus.
Performance evaluation of multi‐precipitation products fusion based on a novel dynamic K‐nearest neighbour Bayesian ensemble calibration framework
Authors: Yang Qiyong , Li Xungui , Sun Jian , Zhang Zhiyong 
Published: June 2024

AbstractThe fusion of multiple precipitation products can effectively improve precipitation accuracy. In order to reduce the uncertainty of traditional precipitation members and improve the applicability of Bayesian model averaging (BMA), this study proposes a dynamic ensemble calibration framework with seasonality and real‐time capability, namely dynamic K‐nearest neighbour BMA (DKBMA), for the integration calibration and comparison of ERA5 reanalysis products and three satellite precipitation products (CMORPH, 3B42RT and 3B42V7). The application results of the DKBMA in the Yujiang River basin, Southern China, during the period of 2011–2016 show that (1) the DKBMA can overcome the problem of ERA5 error interference on the seasonal scale, reduce the systematic bias of ensemble precipitation members at coastal sites and demonstrate strong robustness at different altitudes. (2) Compared with the traditional BMA, the DKBMA has significantly improved accuracy, especially in capturing extreme precipitation events. The correlation coefficient has increased from 0.793 (traditional BMA) to 0.841 (DKBMA), the root‐mean‐square error has decreased from 35.61 (traditional BMA) to 30.95 (DKBMA), and the absolute value of relative bias has decreased from 62.80% (traditional BMA) to 49.94% (DKBMA). The proposed DKBMA in this study can provide a new solution for the fusion of multi‐source precipitation products in data‐scarce regions.
Triamcinolone acetonide has minimal effect on short‐ and long‐term metabolic activities of cartilage
Authors: Porter Annie , Newcomb Emily , DiStefano Steven , Poplawski Jacob , Kim Jonathan , Axe Michael , Lucas Lu Xin 
Published: June 2024

AbstractIntra‐articular corticosteroid injections, such as triamcinolone acetonide (TA), are commonly used by clinicians to manage joint synovial inflammation. However, due to conflicting evidence in literature, there is a fear among clinicians that the injections may be harmful to otherwise healthy cartilage in young patients. The purpose of this study was to evaluate the effects of TA on young, healthy chondrocytes. Articular cartilage samples were harvested from bovine knee joints (1–2 months old). In both healthy and inflammatory (interleukin‐1β) challenged cartilage, samples were treated with TA at doses ranging from 1 nM to 200 μM. Following a short‐ (2 days) or long‐term (10–14 days) treatment, chondrocyte viability, proliferation, and extracellular matrix (ECM) synthesis and degradation were evaluated with a click chemistry‐based technique. Chondrocyte viability, proliferation, and anabolic activity were all minimally affected by short‐term and long‐term TA treatment. After both acute and sustained inflammatory challenges, TA reduced the catabolic activities in cartilage, reducing nascent glycosaminoglycan loss and maintaining cartilage mechanical properties. Overall, at physiologically relevant doses, TA had minimal negative impact on chondrocytes when maintained within their native ECM. Clinical significance: The findings provide new insight for current clinical practices concerning the use of TA in intra‐articular injections, especially in young patients, and established a foundation for future investigations into the impact of corticosteroids on joint homeostasis.
The upcycling of waste frying oil and the effect on sophorolipid productivity
Authors: Ishizaki Ryu , Araki Michiaki , Quan Glen Lelyn , Hirata Yoshihiko 
Published: June 2024

AbstractWaste frying oil (WFO) is a degraded form of oil that is produced from repeated exposure to high temperatures during cooking. Many research studies have focused on the use of recycled WFO. These studies are highly promoted because of the need to find new ways of reducing the negative impact of WFO on the environment. One of these studies focused on the production of sophorolipid (SL), a naturally derived eco‐friendly glycolipid biosurfactant. Unfortunately, the environmental and societal advantages brought about by the wide‐spread use of SL are offset by its high production cost. WFO is a high‐volume, inexpensive material that can be used as a substrate for SL production. As such, by utilizing WFO as a feedstock material cost reduction can be realized to improve large‐scale application potential. One drawback to the use of WFO is that its physical characteristics are different from fresh oil and its effect on SL productivity (g/day) has not been investigated. This research focused on the effect of acid value (AV), peroxide value (PV), and carbonyl value (CV) of WFO on SL productivity. It was confirmed that SL titers (111.1 vs. 106.7 g/L) did not differ significantly after using either fresh oil (CV <0.01) or WFO, respectively. In addition, this research also confirmed that higher WFO degradation required longer culture periods (from 6 days to 9 days) to reach comparable SL concentrations as that produced with fresh oil.
A dream vacation typology
Authors: McKercher Bob 
Published: June 2024

AbstractA six‐fold typology of ideal trips was developed in this study by listening to the voices of residents of Southeast Queensland, Australia, who identified what they felt a dream vacation encompassed. The study represents one of the first of its kind to explore this phenomenon from the tourists' perspective. The typology encompassed 16 styles of holiday. The six types included destination‐exclusive holidays, personal growth, exploration, escape, hedonism and attraction or activity specific holidays. The study concludes that more empirical research into this phenomenon is desired before comprehensive theory development can occur, for existing theories and models used to study ideal holidays tend to be fragmented and often contradictory.
Use of the 445‐nm Blue Laser for Management of Early Glottic Carcinoma: Preliminary 1‐Year Results
Authors: Rosow David E. , Keidar Eytan , Pasick Luke J. , Casellas Nicolas J. , Anis Mursalin M. 
Published: June 2024

ObjectiveTo analyze oncological efficacy and voice outcomes of the 445‐nm blue laser (BL) in the treatment of early glottic carcinoma and compare results with the 532‐nm potassium‐titanyl‐phosphate (KTP) laser.Study DesignSingle institution, retrospective chart review.MethodsAll patients who underwent microlaryngoscopic KTP or BL laser excision of early glottic carcinoma from 2018 to the present day with at least 1‐year follow‐up were included. Primary and recurrent disease, including radiation and surgical failures, were included. Demographic data, voice outcomes and oncologic outcomes were compared between the two laser groups.ResultsForty‐nine patients met the inclusion criteria for the BL group and 88 for the KTP group, with average follow‐up of 635 and 1236 days, respectively. Oncologic outcomes were not significantly different, with disease‐specific survival rates of 95.9% for BL and 100% for KTP (p = 0.13), organ preservation rates of 98.0% for BL and 95.6% for KTP (p = 0.39), and local control rates of 93.9% for BL and 92.1% for KTP (p = 0.81). Both BL and KTP groups showed significant improvement in CAPE‐V (p = 0.04, 0.006 respectively) and VHI‐10 scores (p = 0.003, <0.00001) following surgery.ConclusionsPhotoangiolytic removal of early glottic carcinoma with BL appears to be equally safe and effective as with KTP laser at minimum one‐year follow‐up, and with excellent voice outcomes. Additional study will be warranted over time to assess long‐term outcomes in BL patients.Level of Evidence3 Laryngoscope, 134:4656–4660, 2024
Transient Receptor Potential Ankyrin 1 Channel Alters Transforming Growth Factor Beta 1/Smad Signaling in Rat Vocal Fold Fibroblasts
Authors: Matsushita Hiroki , Mukudai Shigeyuki , Hashimoto Keiko , Kaneko Mami , Sugiyama Yoichiro , Branski Ryan C. , Hirano Shigeru 
Published: June 2024

ObjectivesVocal fold scar remains a therapeutic challenge. Vocal fold fibroblasts (VFFs) secrete extracellular matrix (ECM), and transforming growth factor‐beta 1 (TGF‐β1)‐mediated fibroblast to myofibroblast differentiation is central to the development of fibrosis. The transient receptor potential (TRP) channel superfamily is a group of nonselective cation channels, and activation of TRP ankyrin 1 (TRPA1) channel has been shown to have antifibrotic effects through TGF‐β1/Smad signaling in various organs. This study aimed to elucidate expression of TRPA1 and the impact of TRPA1 activation on TGF‐β1/Smad signaling in VFFs.MethodsVocal folds were dissected from 10‐week‐old, male Sprague‐Dawley rats and primary VFFs were established. TRPA1 was examined in VFFs and lamina propria via immunostaining. VFFs were treated with allyl isothiocyanate (AITC, TRP channel agonist, 10−5 M) ± TGF‐β1 (10 ng/ml) ± A‐967079 (selective TRPA1 channel antagonist, 5.0 × 10−7 M) for 4 or 24 h. Trpa1, Smad3, Smad7, Col1a1, Acta2, and Has1 mRNA expression were quantified via qPCR.ResultsTRPA1 was expressed in cultured VFFs and the lamina propria. TGF‐β1 administration significantly increased Trpa1 compared to control. AITC alone did not alter Smad3, Smad7, Acta2, or ECM related genes. However, the combination of AITC and TGF‐β1 significantly increased Smad3 and decreased Smad7 and Acta2 compared to TGF‐β1 alone; A‐967079 significantly reduced this response.ConclusionsVFFs expressed TRPA1, and the activation of TRPA1 regulated TGF‐β1/Smad signaling in VFFs. These findings provide preliminary insights into potential anti‐fibrotic mechanisms of TRPA1 activation through TGF‐β1/Smad signaling in VFFs.Level of EvidenceNA Laryngoscope, 134:4593–4598, 2024
Can immersive olfactory training serve as an alternative treatment for patients with smell dysfunction?
Authors: Chao Yun‐Ting , Aden Freya , Göktas Önder , Schmidt Friedemann , Göktas Gökhan , Jurkov Miroslav , Georgsdorf Wolfgang , Hummel Thomas 
Published: June 2024

AbstractObjectivesOlfactory training (OT) has emerged as a first‐line therapeutic approach to the management of olfactory dysfunction. Conventional OT (COT) involves the systematic home‐based exposure to four distinct odors. Previous research has demonstrated that immersive OT (IOT) involving full‐body exposure to dozens of distinct odors could also improve overall olfactory function. This study compared IOT and COT in terms of efficacy.MethodsA total of 60 patients were enrolled and assigned to three groups. The IOT group (n = 25) underwent immersive exposure to 64 odors once daily in a specialized theater. COT participants (n = 17) sniffed four typical odors in a set of four jars twice daily at home. A control group (n = 18) underwent passive observation. Olfactory function was assessed before and after training.ResultsSignificant improvements in composite threshold‐discrimination‐identification (TDI) scores were observed after training in both the IOT (mean difference = 2.5 ± 1.1. p = .030) and COT (mean difference = 4.2 ± 1.3, p = .002) groups. No changes were observed in the control group. A significantly higher proportion of patients in the COT group (41%) presented improvements of clinical importance (TDI ≥5.5) compared to the controls (p = .018). The improvements attained in the IOT group (20%) were less pronounced (p = .38).ConclusionWhile IOT did not exhibit the same efficacy as COT in restoring olfactory function, it still demonstrated promising outcomes. Future efforts to advance olfactory recovery should focus on cross‐modal integration.Level of EvidenceLevel 3.
Transparent Multilayer Acrylic Composites Reinforced with Poly(Acrylated Urethane) Filled Low Grammage Bacterial Cellulose Nanopaper
Authors: Wloch Daniela , Herrera Natalia , Lee Koon‐Yang 
Published: June 2024

AbstractCellulose nanopaper is a material structure that possesses high mechanical performance and is widely regarded as a promising 2D reinforcement for polymer matrix composites. This work explores the use of low grammage bacterial cellulose (BC) nanopaper as reinforcement for poly(acrylated urethane) interlayer adhesive to increase the impact performance of multilayer acrylic composites. The BC nanopaper is impregnated with an acrylated urethane resin and laminated between acrylic sheets to create BC/acrylic composites consisting of one, three, and five layers of BC nanopaper‐reinforced poly(acrylated urethane) interlayer adhesive(s). Both the poly(acrylated urethane)‐filled BC nanopaper interlayer adhesive and the resulting laminated acrylic composites are optically transparent. The incorporation of BC nanopaper into the poly(acrylated urethane) interlayer adhesive improves the tensile modulus by eightfold and the single‐edge notched fracture toughness by 60% compared to neat poly(acrylated urethane). It is also found that using poly(acrylated urethane)‐filled BC nanopaper interlayer adhesive proves beneficial to the impact properties of the resulting laminated acrylic composites. In Charpy impact testing, the impact strength of the multilayer acrylic composites increases by up to 130% compared to the “gold‐standard” impact‐modified monolithic acrylic, with a BC loading of only 1.6 wt%.
Potentially functional variants of CHMP4A and PANX1 in the pyroptosis‐related pathway predict survival of patients with non‐oropharyngeal head and neck squamous cell carcinoma
Authors: Tang Xiaozhun , Wang Huiling , Liu Hongliang , Li Guojun , Sturgis Erich M. , Shete Sanjay , Wei Qingyi 
Published: June 2024

AbstractBackgroundPyroptosis has been implicated in the advancement of various cancers. Triggering pyroptosis within tumors amplifies the immune response, thereby fostering an antitumor immune environment. Nonetheless, few published studies have evaluated associations between functional variants in the pyroptosis‐related genes and clinical outcomes of patients with non‐oropharyngeal head and neck squamous cell carcinoma (NON‐ORO HNSCC).MethodsWe conducted an association study of 985 NON‐ORO HNSCC patients who were randomly divided into two groups: the discovery group of 492 patients and the replication group of 493 patients. We used Cox proportional hazards regression analysis to examine associations between genetic variants of the pyroptosis‐related genes and survival of patients with NON‐ORO HNSCC. Bayesian false discovery probability (BFDP) was used for multiple testing correction. Functional annotation was applied to the identified survival‐associated genetic variants.ResultsThere are 8254 single‐nucleotide polymorphisms (SNPs) located in 82 pyroptosis‐related genes, of which 202 SNPs passed multiple testing correction with BFDP < 0.8 in the discovery and six SNPs retained statistically significant in the replication. In subsequent stepwise multivariable Cox regression analysis, two independent SNPs (CHMP4A rs1997996 G > A and PANX1 rs56175344 C > G) remained significant with an adjusted hazard ratios (HR) of 1.31 (95% confidence interval [CI] = 1.09–1.57, p = 0.004) and 0.65 (95% CI = 0.51–0.83, p = 0.0005) for overall survival (OS), respectively. Further analysis of the combined genotypes revealed progressively worse OS associated with the number of unfavorable genotypes (ptrend < 0.0001 and 0.021 for OS and disease‐specific survival, respectively). Moreover, both PANX1 rs56175344G and CHMP4A rs1997996A alleles were correlated with reduced mRNA expression levels.ConclusionsGenetic variants in the pyroptosis pathway genes may predict the survival of NON‐ORO HNSCC patients, likely by reducing the gene expression, but our findings need to be replicated by larger studies.
Application of commercial microwave links (CMLs) attenuation for quantitative estimation of precipitation
Authors: Pasierb Magdalena , Bałdysz Zofia , Szturc Jan , Nykiel Grzegorz , Jurczyk Anna , Ośródka Katarzyna , Figurski Mariusz , Wojtczak Marcin , Wojtkowski Cezary 
Published: June 2024

AbstractPrecipitation estimation models are typically sourced by rain gauges, weather radars and satellite observations. A relatively new technique of precipitation estimation relies on the network of Commercial Microwave Links (CMLs) employed for cellular communication networks: the rain‐inducted attenuation in the links enables the precipitation estimation. In the paper, it is analysed to what extent the precipitation derived from CML attenuation data is useful in estimation of the precipitation field with the high temporal and spatial resolution required in nowcasting models. Two methods of determination of precipitation along CMLs from attenuation of signal with several frequencies were proposed. Then, in order to generate precipitation field, three approaches for assigning appropriate precipitation values to a specific point or set of pixels along the link are developed and tested. The CML‐based estimates are compared with point observations from manual rain gauges and multi‐source precipitation fields using daily and half‐hourly accumulations. It was found that the CML‐based precipitation fields are much worse than radar‐derived estimates. At the same time, they had slightly poorer reliability than spatially interpolated telemetric rain gauge data and significantly higher reliability than satellite estimates. Furthermore, the impact of link characteristics, such as length and frequency, on the reliability of CML‐based precipitation estimates is analysed.
Social media use by patients with hypermobile Ehlers–Danlos syndrome
Authors: Halverson Colin M. E. , Doyle Tom A. , Vershaw Samantha 
Published: June 2024

AbstractBackgroundPatients with uncommon genetic conditions often face limited in‐person resources for social and informational support. Hypermobile Ehlers–Danlos syndrome (hEDS) is a rare or underdiagnosed hereditary disorder of the connective tissue, and like those with similar diseases, patients with hEDS have begun to turn to social media in search of care and community. The aims of our study were to understand the usage habits and perceptions of utility of social media use for patients with hEDS in order to formulate suggestions for how clinicians may best engage these and similar patient populations about this topic.MethodsWe conducted both a quantitative survey and qualitative interviews with patients who had received a robust clinical diagnosis of hEDS.ResultsTwenty‐four individuals completed the initial survey, and a subset of 21 of those participants completed an interview. Through thematic analysis, we identified four primary themes related to their experience with social media: (1) befriending others with their disease, (2) seeking and vetting information, (3) the risks and downsides of social media use, and (4) the desire for clinicians to discuss this topic with them.ConclusionWe conclude by proposing five suggestions that emerge empirically from our data. These proposals will help clinicians engage their patients regarding social media use in order to promote its potential benefits and circumvent its potential harms as they pursue support for their hereditary condition.