bluuebunny/crossref_metadata_2025_split huggingface dataset:
Principles and methodology for translation and cross‐cultural adaptation of the Nordic Occupational Skin Questionnaire (NOSQ‐2002) to Spanish and Catalan
Authors: Sala‐Sastre Nohemi , Herdman Mike , Navarro Lidia , De LaPRADA Miriam , Pujol RAMÓN M. , Serra Consol , Alonso Jordi , Flyvholm Mari‐Ann , GIMÉNEZ‐Arnau Ana M. 
Published: August 2009

Background: Occupational skin diseases are among the most frequent work‐related diseases in industrialized countries. The Nordic Occupational Skin Questionnaire (NOSQ‐2002), developed in English, is a useful tool for screening of occupational skin diseases.Objectives: To culturally adapt the NOSQ‐2002 to Spanish and Catalan and to assess the clarity, comprehension, cultural relevance and appropriateness of the translated versions.Methods: The International Society for Pharmacoeconomics and Outcomes Research (ISPOR) principles of good practice for the translation and cultural adaptation of patient‐reported outcomes were followed.Results: After translation into the target language, a first consensus version of the questionnaire was evaluated in multiple cognitive debriefing interviews. The expert panel introduced some modifications in 39 (68%) and 27 (47%) items in the Spanish and Catalan version, respectively (e.g. addition of examples and definitions, reformulation of instructions and use of direct question format). This version was back translated and submitted to the original authors, who suggested a further seven and two modifications in the Spanish and Catalan versions, respectively. A second set of cognitive interviews were performed. A consensus version of both questionnaires was obtained after final modifications based on comments by the patients.Conclusions: The final versions of the Spanish and Catalan NOSQ‐2002 questionnaires are now available at www.NRCWE.dk/NOSQ.
Allergens associated with severe symptoms of hand eczema and a poor prognosis
Authors: Hald Marianne , Agner Tove , Blands Jette , Ravn Henrik , Johansen Jean Duus 
Published: August 2009

Background: Contact allergy is frequent among persons with hand eczema and may be associated with a poor prognosis.Objectives: To identify allergens associated with the most severe initial clinical symptoms and the worst prognosis in a cohort of hand eczema patients followed for 6 months.Methods: The study population comprised 799 consecutive hand eczema patients enrolled during January 2006–February 2007. All patients were patch tested with the European baseline series. Severity assessment of the hand eczema was performed initially and at the 6‐month follow‐up using a validated scoring system (HECSI). With logistic regression analyses, associations of severe hand eczema or a poor prognosis with 15 individual allergens were analysed and adjusted for by sex, age, atopic dermatitis and other allergens.Results: At baseline, greater severity of hand eczema was associated with a positive patch test to formaldehyde, methyldibromo glutaronitrile, sesquiterpene lactone mix, nickel sulfate and potassium dichromate. A poor prognosis was associated with chromate allergy, odds ratio: 4.18 (95% CI: 1.42–12.28).Conclusions: Nickel, chromate, formaldehyde, methyldibromo glutaronitrile and sesquiterpene lactone mix were allergens associated with the greatest severity of hand eczema. Patients with chromate allergy had the worst prognosis.
Accumulation of FITC near stratum corneum–visualizing epidermal distribution of a strong sensitizer using two‐photon microscopy
Authors: Samuelsson Kristin , Simonsson Carl , Jonsson Charlotte A. , Westman Gunnar , Ericson Marica B. , Karlberg Ann‐Therese 
Published: August 2009

Background: The allergenic potency of a hapten is related to its skin penetration properties, but little is known about the distribution of haptens in the skin following topical application.Objectives: The aim of this study was to investigate the diffusion and epidermal distribution using two‐photon microscopy (TPM) of two fluorescent compounds.Methods: Sensitizing capacities of fluorescein isothiocyanate (FITC) and fluorescein were investigated using the local lymph node assay. Chemical reactivity of the compounds was analysed, and their distribution in human epidermis was visualized using TPM and confocal microscopy. Also the in vitro diffusion through epidermis of FITC and fluorescein has been examined.Results: FITC was classified as an extreme sensitizer, whereas fluorescein was non‐sensitizing. TPM and confocal microscopy showed an accumulation of FITC in stratum corneum (SC), whereas fluorescein was more evenly distributed in epidermis. The diffusion of fluorescein through epidermis was three times higher than that of FITC.Conclusions: TPM, which has never been used in this context before, is a promising tool for visualizing the distribution of fluorescent compounds of varying reactivity in intact skin. The strong allergen FITC is mainly retained in or adjacent to SC, whereas most fluorescein diffused through the epidermis.
Development of lichen sclerosus et atrophicus while receiving a therapeutic dose of imatinib mesylate for chronic myelogenous leukemia
Authors: Skupsky Hadas , Abuav Rachel , High Whitney , Pass Carolyn , Goldenberg Gary 
Published: August 2009

Background: Imatinib mesylate (Gleevec®) is a selective Bcr‐Abl protein tyrosine‐kinase inhibitor, and it also inhibits the receptor tyrosine kinases for stem cell factor (c‐kit) and platelet‐derived growth factor (PDGFR). It is being investigated for use in the treatment of sclerosing dermatoses. Observation: A 44‐year‐old woman with a history of chronic myelogenous leukemia (CML) was referred for the evaluation of a pruritic eruption that developed over 6 months. Examination revealed atrophic plaques confined to the groin, vulva, axillae, inframammary region, trunk, antecubital and popliteal fossae, and posterior thighs bilaterally. A biopsy showed lichen sclerosus et atrophicus (LSetA). At the time of presentation, the patient was receiving imatinib mesylate 400 mg daily for CML. Conclusion: This is the first report of development of LSetA, a sclerosing dermatosis, while receiving a therapeutic dose of imatinib mesylate, a drug thought to have anti‐sclerotic properties.Skupsky H, Abuav R, High W, Pass C, Goldenberg G. Development of lichen sclerosus et atrophicus while receiving a therapeutic dose of imatinib mesylate for chronic myelogenous leukemia.
Epithelioid hemangioma (angiolymphoid hyperplasia with eosinophilia) arising on the extremities
Authors: Cham Elaine , Smoller Bruce R. , Lorber David A. , Victor Thomas A. , Cibull Thomas L. 
Published: August 2009

Background: Epithelioid hemangioma (EH) is a benign vascular proliferation usually accompanied by a mixed inflammatory infiltrate. Methods: Skin biopsy specimens from four patients with EH on the extremities were studied. Architecture, extent of vascular proliferation and the presence of epithelioid endothelial cells were evaluated. The features of the inflammatory infiltrate were also assessed, including the distribution, depth, predominant cell type, presence of germinal centers, and distribution and number of CD30+ cells. Results: All cases showed the typical lobular pattern of small vessels centered about a ‘feeder’ vessel. Larger vessels were lined by epithelioid endothelial cells. The mixed inflammatory infiltrate was nodular, perivascular and periadnexal. Germinal centers were seen in two cases. Large activated CD30+ lymphocytes were seen in all cases. Conclusions: EH can lead to diagnostic confusion with cutaneous lymphoma and other entities, especially when its mixed inflammatory infiltrate predominates over its vascular component and contains large activated CD30+ lymphocytes. Awareness that the presence of CD30+ activated lymphocytes is not specific for lymphoma and recognition of the vascular component is critical for proper diagnosis of EH.Cham E, Smoller BR, Lorber DA, Victor TA, Cibull TL. Epithelioid hemangioma (angiolymphoid hyperplasia with eosinophilia) arising on the extremities.
Characterization of cytotoxic immune response in skin and mucosal lesions of paracoccidioidomycosis
Authors: Pagliari Carla , Pereira Naiura Vieira , Kanashiro Luciane , Stegun Felipe Weisshaupt , Croda Julio , Duarte Maria Irma Seixas , Sotto Mirian Nacagami 
Published: August 2009

Background: CD8+ T cells and natural killer (NK) cells are involved in the immune response against some pathogens. For this purpose, we investigated the in situ paracoccidioidomycosis (PCM) immune response addressing the participation of NK cells, CD8+ T cells, perforin and granzyme B expression. Methods: Sixty biopsies of PCM skin and mucosa were classified according to the presence of compact granulomas (G1), poorly organized granulomas (G2) and both kinds in the same lesion (G3). CD8+ T cells, NK cells, perforin and granzyme B were showed by immunohistochemistry. Results: CD8+ T cells were increased over NK cells in cutaneous G1 and G2 lesions. There was no difference regarding such cells in G3 lesions, although they were abundant in such lesions. In mucosa, CD8+ T cells were increased in number over NK cells in all groups. Granzyme B in skin increased in G2 and G3. The number of granzyme did not differ in mucosal lesions in the three groups. Conclusions: CD8+ T cells and NK cells play a role in PCM cutaneous and mucosal lesions. The predominance of CD8+ T cells over NK cells may represent an effective response against the fungi. Moreover, the high number of granzyme B expressing cells corroborates this possibility.Pagliari C, Pereira NV, Kanashiro L, Stegun FW, Croda J, Seixas Duarte MI, Sotto MN. Characterization of cytotoxic immune response in skin and mucosal lesions of paracoccidioidomycosis.
Improved quality of optical coherence tomography imaging of basal cell carcinomas using speckle reduction
Authors: Mogensen Mette , Jørgensen Thomas Martini , Thrane Lars , Nürnberg Birgit Meinecke , Jemec Gregor Borut Ernst 
Published: August 2009

Please cite this paper as: Improved quality of optical coherence tomography imaging of basal cell carcinomas using speckle reduction. Experimental Dermatology 2010; 19: e293–e295.AbstractBackground:  Optical coherence tomography (OCT) is a possible imaging method for delineation of non‐melanoma skin cancer. Speckle noise is the dominant noise contribution in OCT images; it limits the ability to identify cellular structures especially skin cancer.Questions addressed:  This report suggests a method for improving OCT image quality for skin cancer imaging.Experimental design:  OCT is an optical imaging method analogous to ultrasound. Two basal cell carcinomas (BCC) were imaged using an OCT speckle reduction technique (SR‐OCT) based on repeated scanning by altering the distance between the probe and the surface of the skin.Results:  SR‐OCT resulted in improved visualisation and more accurate thickness measurements in BCC lesions.Conclusion:  This OCT speckle reduction method led to improved visualisation and better defined delineations in two BCC lesions. Thus, OCT was improved to a clinically relevant level when imaging BCC lesions.
Stretching stimulates fibulin‐5 expression and controls microfibril bundles in human periodontal ligament cells
Authors: Nakashima K. , Tsuruga E. , Hisanaga Y. , Ishikawa H. , Sawa Y. 
Published: August 2009

Background and Objective:  The elastic fiber system comprises oxytalan, elaunin and elastic fibers, differing in their relative microfibril and elastin contents. Human periodontal ligaments contain oxytalan fibers (pure microfibrils). Periodontal ligaments are continuously exposed to various functional forces, such as tooth movement and occlusal loading. We have reported that bundles of microfibrils coalesce in response to mechanical strain in cultured periodontal ligament fibroblasts, as assessed in terms of their positivity for fibrillin‐1 (the major component of microfibrils). However, the mechanism of microfibril coalescence is unclear. We hypothesized that the fibrillin‐1‐binding molecule, fibulin‐5, contributes to oxytalan fiber formation under mechanical strain.Material and Methods:  We subjected periodontal ligament fibroblasts to stretching in order to examine the effects of fibulin‐5 on the formation of oxytalan fibers in cell/matrix layers. We transfected periodontal ligament cells with small interference RNA for fibulin‐5, then examined oxytalan fibers using immunofluorescence and electron microscopy.Results:  Immunofluorescence showed that fibrillin‐1‐positive microfibrils coalesced as a result of stretching, compared with cells that were not subjected to stretching. Fibulin‐5 colocalized on fibrillin‐1‐positive microfibrils. Stretching increased fibulin‐5 gene expression and protein deposition. Immunofluorescence and immunogold electron microscopy analysis revealed that fibulin‐5 suppression inhibited the coalescence of microfibrils under stretching conditions.Conclusion:  These results suggest that fibulin‐5 up‐regulated in response to tension strain may control the formation of microfibril bundles in periodontal ligament.
ORIGINAL ARTICLE: Endogenous Adenosine Down‐Modulates Mid‐Trimester IntraAmniotic Tumor Necrosis Factor‐α Production
Authors: Perni Uma , Sezen Devrim , Bongiovanni Ann Marie , Linhares Iara M. , Skupski Daniel , Witkin Steven S. 
Published: August 2009

Problem  To determine whether adenosine in amniotic fluid down‐regulates pro‐inflammatory cytokine production.Method of study  Mid‐trimester amniotic fluid from 21 women was incubated ex vivo in the presence or absence of human adenosine deaminase, the enzyme that irreversibly degrades adenosine. After 24 hr, supernatants were assayed by ELISA for tumor necrosis factor‐α (TNF‐α), interleukin (IL)‐6, and IL‐10. Clinical parameters were obtained after completion of laboratory testing.Results  Inclusion of adenosine deaminase resulted in a median increase in TNF‐α production from 0.9 to 7.3 pg/mL (P = 0.0014). IL‐6 production exhibited a non‐significant median increase from <2.0 to 53.0 pg/mL (P = 0.0780). Median IL‐10 production increased slightly from a median of <0.2 to 1.3 pg/mL. Adenosine deaminase‐stimulated TNF‐α production was proportional to parity and unrelated to gestational age, time of delivery, maternal age or indication for amniocentesis.Conclusion  Adenosine deaminase treatment increases TNF‐α production by ex vivo‐cultured amniotic fluid. Adenosine contributes to immune modulation in the amniotic cavity.
Intravascular papillary endothelial hyperplasia of the lower lip: surgical approach and review of the literature
Authors: Cohen A. , Maly A. , Azaz B. 
Published: August 2009

Objective:  A review of the literature for intravascular papillary endothelial hyperplasia is presented along with a case report of a geriatric patient.Review of the literature:  Intravascular papillary endothelial hyperplasia (IPEH) is a reactive benign lesion of vascular origin, which is caused by an excessive proliferation of endothelial cells. Only a few cases with IPEH in the oral cavity have been recorded in the literature, reporting the lower lip as the main site. The treatment of choice mentioned in the literature is simple excision.Case report:  In this case, an IPEH of the lower lip of a 79‐year‐old male was treated by a sclerosing agent, which was injected into the lesion, causing compression and fibrosis of the blood vessels, followed by a careful dissection and excision. Intra‐operatively no bleeding occurred. Post‐operatively an excellent aesthetic result was achieved, without recurrence.Conclusion:  The use of sclerotherapy followed by surgery in mixed type intravascular papillary endothelial hyperplasia can provide an acceptable aesthetic result with minimal intra‐operative bleeding.
Prevalence of oral and dental disorders in institutionalised elderly people in Rasht, Iran
Authors: Rabiei Maryam , Kasemnezhad Ehsan , Masoudi rad Hossein , Shakiba Maryam , Pourkay Hossein 
Published: August 2009

doi:10.1111/j.1741‐2358.2009.00313.x

Prevalence of oral and dental disorders in institutionalised elderly people in Rasht, Iran Objective:  The aim of this study was to determine the prevalence of dental and oral mucosal lesions in institutionalised elderly people in Rasht.Background:  Oral health in the elderly people is important to tailor the health programmes for this increasing age group within the population.Methods:  A total of 216 elderly people residing in a geriatric nursing home were included in the study. Subjects were interviewed and examined clinically and registered in a data collection form and analysed using spss version 11 program (SPSS Inc, Chicago, Illinois, USA).Results:  The prevalence of dental and oral disorders was 44.9% and 86.1% respectively. The most frequent oral disorders were dry mouth (42.1%), fissure tongue (25.9%), atrophic of tongue (25%), sublingual varicosity (22.7%), burning sensation (16.7%) and varix (15.3%). The prevalence of edentulousness was 56% (95%CI: 49–63%). The mean level of retained teeth was 3.22. The odds of an oral mucosal disorder in females were significantly more than in males (OR = 2.79, 95%CI: 1.25–6.23).Discussion:  The findings revealed a high prevalence of dental and oral disorders in institutionalised elderly people in Rasht in comparison to similar studies. The mean of retained teeth was also much lower than in other reports. Therefore, the necessity for regular oral examination of these rapidly expanding age groups of people is essential.
Number of teeth and selected cardiovascular risk factors among elderly people
Authors: Syrjälä Anna‐Maija Hannele , Ylöstalo Pekka , Hartikainen Sirpa , Sulkava Raimo , Knuuttila Matti 
Published: August 2009

doi:10.1111/j.1741‐2358.2009.00328.x

Number of teeth and selected cardiovascular risk factors among elderly people Objective:  To produce evidence on an association between the number of teeth and selected cardiovascular risk factors among an elderly population.Materials and methods:  The study population comprised of 523 community‐living elderly people who participated in the population‐based Kuopio 75+ study. The data for each subject were collected using a structured clinical health examination, an interview and laboratory tests. Linear regression models were used to estimate adjusted mean values and confidence limits.Results:  Edentulous persons and persons with a small number of teeth had lower serum HDL cholesterol and higher triglyceride, leucocyte and blood glucose levels and a higher body mass index (BMI) compared with subjects to a large number of teeth.Conclusion:  The study showed that, in the Finnish home‐dwelling population aged 75 years or older, those with a large number of teeth were less likely to have cardiovascular risk factors such as a low serum HDL cholesterol level, a high triglyceride level and a high BMI than did subjects with a small number of teeth or who were edentulous.
EFFECT OF COOKING ON THE PROFILE OF PHENOLICS, TANNINS, PHYTATE, AMINO ACID, FATTY ACID AND MINERAL NUTRIENTS OF WHOLE‐GRAIN AND DECORTICATED VEGETABLE COWPEA (VIGNA UNGUICULATA L. WALP)
Authors: ADEBOOYE ODUNAYO CLEMENT , SINGH VASUDEVA 
Published: August 2009

ABSTRACT An experiment was conducted to determine the profile of phenolics, tannins, phytate, amino acid, fatty acid and mineral nutrients in whole‐grain and decorticated vegetable cowpea before and after cooking. Two cowpea varieties: C‐152‐White, having big grain and S‐1552‐White, having small grain with black eye were used for the study. Decortication of cowpea grain resulted in significant reduction (P ≤ 0.05) of total phenolics, total tannins and phytate contents of the two cowpea varieties. Comparison showed that cooking resulted in 19, 28, 28 and 37% losses in phenolics in whole‐grain C‐152, decorticated C‐152, whole‐grain S‐1552 and decorticated S‐1552, respectively. After cooking, whole‐grain and decorticated C‐152 retained 52.1 and 57.7% tannins while S‐1552 retained 59.4 and 49.5%, respectively. In the whole grain, C‐152 lost 72.2% phytate after cooking while S‐1552 lost 70.3%. For the decorticated samples, C‐152 lost 72.4% phytate after cooking while S‐1552 lost 71.2%. For the two cowpea varieties, decortication resulted in significant (P ≤ 0.05) losses of Ca, Fe, Mn, Mg, Zn, K and Cu while there was no significant effect on protein and amino acid profile. Cooking did not cause any significant change in the mineral nutrient composition of either the whole‐grain or decorticated cowpea. For the two cowpea varieties, bulk of the fat consisted of palmitic acid (23.2–27.3%) and stearic acid (53.5–59.8%) in the ratio of 1:2, approximately. Upon cooking, losses of crude protein were recorded in the whole‐grain and decorticated samples of the two cowpea varieties.PRACTICAL APPLICATIONSCowpea is an important cheap source of protein particularly for the low‐income countries, especially in Africa and Asia. However, there is the need for some pretreatments before its cooking because of the presence of some undesirable antinutrients which often cause flatulence after consumption or they can render some minerals unavailable for body assimilation. This study investigated the role that African traditional method of decortication and cooking could play in reducing the concentration of antinutritional factors. We also showed how the traditional treatments could affect the other nutritionally important component of cowpea.
EFFECTS OF GLUCOSE OXIDASE, HEMICELLULASE AND ASCORBIC ACID ON DOUGH AND BREAD QUALITY
Authors: DAGDELEN ADNAN FATIH , GOCMEN DUYGU 
Published: August 2009

ABSTRACT In this study, glucose oxidase alone or its combinations with hemicellulase or ascorbic acid were used in bread making. Glucose oxidase alone mainly decreased dough extensibility. It produced stiffer and less extensible dough. Combinations of glucose oxidase–hemicellulase presented lower extensibility and were more resistant to extension than glucose oxidase alone. When glucose oxidase–ascorbic acid combinations were used, the softening degree significantly decreased, regardless when added the lowest glucose oxidase in combination with ascorbic acid. Glucose oxidase–ascorbic acid combinations significantly modified dough resistance. The glucose oxidase alone significantly increased specific loaf volume. The Dallman value of loaves made with glucose oxidase alone was found higher than for control. The most dramatic effect of additives on specific loaf volume was observed when glucose oxidase–hemicellulase combinations were added. This effect has been ascribed to redistribution of water from the hemicellulose to gluten, which would render the gluten more extensible. Specific loaf volume showed a significant enhancement when glucose oxidase–ascorbic acid combinations were added, but this effect was not as good as glucose oxidase–hemicellulase. The effects of glucose oxidase and its combinations with ascorbic acid and hemicellulase on dough rheology and bread quality are highly dependent on the amount of enzyme and the original wheat flour quality. PRACTICAL APPLICATIONIn practice, appropriate combinations of glucose oxidase with hemicellulase can be used as improvers in bread making, depending on their combination levels. This study will show the way to new research about glucose oxidase, ascorbic acid and hemicellulose.