bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruption of the CRF1receptor eliminates morphine-induced sociability deficits and firing of oxytocinergic neurons in male mice
Authors: Piccin Alessandro , Allain Anne-Emilie , Baufreton Jérôme , Bertrand Sandrine S. , Contarino Angelo 
Published: June 2024

AbstractSubstance-induced social behavior deficits dramatically worsen the clinical outcome of substance use disorders; yet, the underlying mechanisms remain poorly understood. Herein, we investigated the role for the corticotropin-releasing factor receptor 1 (CRF1) in the acute sociability deficits induced by morphine and the related activity of oxytocin (OXY)-and arginine-vasopressin (AVP)-expressing neurons of the paraventricular nucleus of the hypothalamus (PVN). For this purpose, we used both the CRF1receptor-preferring antagonist compound antalarmin and the genetic mouse model of CRF1receptor-deficiency. Antalarmin completely abolished sociability deficits induced by morphine in male, but not in female, C57BL/6J mice. Accordingly, genetic CRF1receptor-deficiency eliminated morphine-induced sociability deficits in male mice.Ex vivoelectrophysiology studies showed that antalarmin also eliminated morphine-induced firing of PVN neurons in male, but not in female, C57BL/6J mice. Likewise, genetic CRF1receptor-deficiency reduced morphine-induced firing of PVN neurons in a CRF1gene expression-dependent manner. The electrophysiology results consistently mirrored the behavioral results, indicating a link between morphine-induced PVN activity and sociability deficits. Interestingly, in male mice antalarmin abolished morphine-induced firing in neurons co-expressing OXY and AVP, but not in neurons expressing only AVP. In contrast, in female mice antalarmin did not affect morphine-induced firing of neurons co-expressing OXY and AVP or only OXY, indicating a selective sex-specific role for the CRF1receptor in opiate-induced PVN OXY activity. The present findings demonstrate a major, sex-linked, role for the CRF1receptor in sociability deficits and related brain alterations induced by morphine, suggesting new therapeutic strategy for opiate use disorders.
Consistent neural representation of valence in watching and recall conditions
Authors: Kim Hyeonjung , Kim Jongwan 
Published: June 2024

AbstractRecall is an act of elicitation of emotions similar to those emotions previously experienced. Unlike the past experiences where external sensory stimuli triggered emotions, recall does not require external sensory stimuli. This difference is pertinent to the key debate in affective representation, addressing whether the representation of valence is consistent across modalities (modality-general) or dependent on modalities (modality-specific). This study aimed to verify neural representations of valence irrespective of the presence of external sensory stimuli. Using neuroimaging data from video watching and recall (Chen et al., 2017) and behavioral data for valence ratings (Kim et al., 2020), a searchlight analysis was conducted with cross-participant regression-based decoding across the presence and absence of external stimuli. Multidimensional scaling was employed as a validation analysis of the results. The searchlight analysis revealed the right middle temporal and inferior temporal gyrus as well as the left fusiform gyrus. The validation analysis further exhibited significant consistent neural representations of valence in the inferior temporal gyrus and the left fusiform gyrus. This study identified the brain regions where valence is consistently represented, regardless of the presence of external sensory stimuli. These findings contribute to debate in affective representations, by comparing conditions utilized little in prior, suggesting the inferior temporal gyrus is related to representations of valence irrespective of the presence and absence of external visual stimuli.
A Cell-Permeable Fluorescent Probe Reveals Temporally Diverse PI(4,5)P2 Dynamics Evoked by Distinct GPCR Agonists in Neurons
Authors: Kundu Rajasree , Mondal Samsuzzoha , Kapadia Akshay , Banerjee Antara A. , Kucherak Oleksandr A. , Klymchenko Andrey S. , Koushika Sandhya P. , Venkatramani Ravindra , Vaidya Vidita A. , Datta Ankona 
Published: June 2024

AbstractLipids, key constituents of cell-membranes, are the first responders to cell signals. At the crux of spatiotemporal dynamics of lipid-signaling response are phosphoinositides. Indeed, phosphoinositides like phosphatidylinositol-(4,5)-bisphosphate (PI(4,5)P2), present in the inner-leaflet of eukaryotic cell-membranes, form the link between signal reception and downstream signal-transmission. In this backdrop, reversible fluorescent probes that can track live PI(4,5)P2 dynamics on a seconds time-scale will afford key insights into lipid-mediated signaling. However, realizing cell-permeable PI(4,5)P2-selective sensors for imaging dynamics remains a challenge due to the presence of structurally similar lipids and low levels of PI(4,5)P2. We report a computationally-designed, rapid-response, reversible, photo-stable, fluorescent sensor that permeates living cells, neurons, and a multicellular organism within few min of direct incubation and distinctly visualizes PI(4,5)P2 pools. We used the sensor to interrogate the role of PI(4,5)P2 in driving heterogeneity of signaling responses and contrasting behavioral effects that ensue upon binding of distinct ligands to the same G protein-coupled receptor. Specifically, we asked whether probing PI(4,5)P2 dynamics using our novel sensor could uncover the earliest of signaling differences evoked by hallucinogenic versus non-hallucinogenic ligands at the serotonin2A (5-HT2A) receptor. Our results reveal that a hallucinogenic ligand at the 5-HT2Areceptor leads to a slower rate of PI(4,5)P2-depletion when compared to a non-hallucinogenic ligand, within the initial seconds of ligand addition, but has a sustained effect. The ability of our designer chemical probe in timing early seconds-minute timescale lipid-dynamics in living cells opens avenues for tracking early time-point molecular events in neuronal response to chemical and physical stimuli.
Comparative Nephro-Hepatoprotective Effects of Herbal Teas fromHibiscus sabdariffa, Moringa oleifera, Zingiber officinale, and Azadirachta indicain Alloxan-Induced Diabetic Male Wistar Rats: A Preclinical Study
Authors: Numonde Emieseimokumo , Momoh Isaac Sokoato , Sheneni Victor Duniya , Okosesi Ebunoluwa Ajibike , Ibrahim Micheal Omeyiza 
Published: June 2024

AbstractDiabetes, a silent killer, ravages the kidney and liver, leaving a trail of destruction in its wake. Studies have suggested a linkconnectingdysfunctional liver and renalactivity along side glucotoxicretinopathy in patients of type 2 diabetes mellitus. Against this background, our preclinical study unveils the remarkable nephro-hepatoprotective effects of herbal teas fromHibiscus sabdariffa, Moringa oleifera, Zingiber officinale, and Azadirachta indicain diabetic male Wistar rats induced with alloxan.Diabetes of artificial source was administered through a one-time interperitonealadministeration of about seven categorieshaving 6 rats in the individual group.control (glycemic), diabetic groupthat is made up of alloxan treatment + Zobo (100+400 mg/kg)., alloxan + moringa (100 +200 mg/kg), alloxan + ginger (100+500 mg/kg), alloxan + Dogoyaro (100+250 mg/kg), and lastly, the alloxan +glibenclamide(100+5mg/kg) were orally given for 28 days.Reduction of tissue weight was observed upon administration of alloxan which was ameliorated upon treatment with the selected herbal teas. Also, elevated levels of liver and kidney biomakersinduced by alloxan were reversed upon administration of the Herbal teas. Furthermore, the Herbal teas decreased fasting blood glucose, which was initially significantly increased byalloxan (p<0.05). Consequently, given to their antihyperglycemic and nephro-hepatoprotective prowess, these selected herbal teas offer a beacon of hope for the millions afflicted not just with diabetes but also diabetes-related kidney and liver diseases.
Diversifying selection and adaptive introgression of carotenoid-processing genes underlie the evolution of bill color in the long-tailed finch
Authors: Hooper Daniel M. , McDiarmid Callum S. , Powers Matthew J. , Justyn Nicholas M. , Kučka Marek , Hart Nathan S. , Hill Geoffrey E. , Andolfatto Peter , Chan Yingguang Frank , Griffith Simon C. 
Published: June 2024

AbstractCarotenoid pigmentation produces the yellow and red coloration of birds and other vertebrates, but our understanding of the genetic architecture of carotenoid ornamentation is largely limited to studies of novel color variants observed in captively bred populations. The complexity of carotenoid-based color evolution in nature remains poorly characterized. Here, we examine the long-tailed finchPoephila acuticauda, an Australian songbird with two hybridizing subspecies that differ in bill coloration: yellow in western subspeciesacuticaudaand red in eastern subspecieshecki. We characterize the carotenoid composition of each subspecies and find that yellow bills can be explained by the loss of C(4)-oxidation, thus blocking yellow dietary pigments from being metabolized to red. Combining linked-read genomic sequencing and reflectance spectrophotometry measurements of bill color collected from wild-sampled finches and laboratory crosses, we identify four loci that together explain 53% of variance in this trait. The two loci of largest effect contain the genesCYP2J19, an essential enzyme for the ketolation via C(4)-oxidation of dietary carotenoids, andTTC39B, an enhancer of ketocarotenoid production. Evolutionary genealogy reconstruction indicates that the red-billed phenotype is ancestral and yellow alleles at bothCYP2J19andTTC39Barose and fixed inacuticaudaapproximately 100 kya. Yellow alleles then introgressed intoheckiless than 5 kya. Across all four loci,acuticaudaderived variants show evidence of selective sweeps, implying that yellow bill coloration has been favored by natural selection. Our study suggests that the frequent adaptive evolutionary transitions between red and yellow ornamentation in nature can have a simple genetic basis.SignificanceWe studied variation in carotenoid ornamentation of an Australian songbird with two hybridizing subspecies that differ in bill color: one yellow and the other red. We identified a single metabolic process, C(4)-oxidation, underlying the distinct carotenoid composition of these two bill colors. Genetic association mapping revealed four major effect loci that explained most of the observed variation the trait, including the oxidative ketolation enzymeCYP2J19and the carotenoid ketolation enhancer geneTTC39B. Evolutionary reconstruction indicates that yellow alleles are derived, ancient (~100 kya), and under positive selection. This has driven their recent (<5 kya) adaptive introgression across the hybrid zone. These findings have important implications for understanding the role of natural selection in phenotypic evolution in natural systems.
Modulation ofSaprolegnia parasiticagrowth with copper and ionophores
Authors: Ogunwa Tomisin Happy , Thornhill Madison Grace , Ledezma Daniel , Peterson Ryan Loren 
Published: June 2024

AbstractSaprolegnia parasiticais an oomycete pathogen responsible for saprolegniasis diseases that result in large production losses in the catfish and salmon aquaculture industry. The use of copper sulfate as an anti-Saprolegnia treatment has been reported as an alternative to malachite green, formaldehyde and hydrogen peroxide treatment methods. The current study investigates a new strategy to inhibitSaprolegnia parasiticagrowth by combining copper and ionophores at low levels. The chemical agents tetraethylthiuram disulfide (TDD), ciclopirox olamine (CLP), 2-mercaptopyridine N-oxide (MPO), 5-chloro-8-hydroxy-7-iodoquinoline (CHI), 5,7-dichloro-8-hydroxyquinoline (DHQ) and 8-Quinolinol (8QN) were identified to inhibitS. parasiticagrowth in a copper-dependent manner. At concentrations below the lethal dose of individual ionophore, increasing copper concentrations resulted in synergeticS. parasiticagrowth inhibition. The addition of the exogenous copper chelator bathocuproine sulfate (BCS), reversed the inhibition ofS. parasiticagrowth by TDD, CLP, MPO, and 8QN but not CHI and DHQ. Our data demonstrates that ionophores, in combination with low levels of copper, can effectively limitS. parasiticagrowth both in a liquid and solid support growth environment. Investigations into the underlying mechanism of Cu-ionophore toxicity are discussed.
Extensive location bias of the GPCR-dependent translatome via site-selective activation of mTOR
Authors: Klauer Matthew J , Jagla Caitlin AD , Tsvetanova Nikoleta G 
Published: June 2024

ABSTRACTG protein-coupled receptors (GPCRs) modulate various physiological functions by re-wiring cellular gene expression in response to extracellular signals. Control of gene expression by GPCRs has been studied almost exclusively at the transcriptional level, neglecting an extensive amount of regulation that takes place translationally. Hence, little is known about the nature and mechanisms of gene-specific post-transcriptional regulation downstream of receptor activation. Here, we apply an unbiased multiomics approach to delineate an extensive translational regulatory program initiated by the prototypical beta2-adrenergic receptor (β2-AR) and provide mechanistic insights into how these processes are orchestrated. Using ribosome profiling (Ribo-seq), we identify nearly 120 novel gene targets of adrenergic receptor activity which expression is exclusively regulated at the level of translation. We next show that all translational changes are induced selectively by endosomal β2-ARs. We further report that this proceeds through activation of the mammalian target of rapamycin (mTOR) pathway. Specifically, within the set of translational GPCR targets we discover significant enrichment of genes with 5’ terminal oligopyrimidine (TOP) motifs, a gene class classically known to be translationally regulated by mTOR. We then demonstrate that endosomal β2-ARs are required for mTOR activation and subsequent mTOR-dependent TOP mRNA translation. Together, this comprehensive analysis of drug-induced translational regulation establishes a critical role for location-biased GPCR signaling in fine-tuning the cellular protein landscape.
Systemic delivery of murine SOD2 mRNA to experimental abdominal aortic aneurysm mitigates expansion and rupture
Authors: Yan Huimin , Hu Ying , Lyu Yang , Akk Antonina , Hirbe Angela C. , Wickline Samuel A. , Pan Hua , Roberson Elisha D.O. , Pham Christine T.N. 
Published: June 2024

AbstractBackgroundOxidative stress is implicated in the pathogenesis and progression of abdominal aortic aneurysm (AAA). Antioxidant delivery as a therapeutic for AAA is of substantial interest although clinical translation of antioxidant therapy has met with significant challenges due to limitations in achieving sufficient antioxidant levels at the site of AAA. We posit that nanoparticle-based approaches hold promise to overcome challenges associated with systemic administration of antioxidants.MethodsWe employed a peptide-based nanoplatform to overexpress a key modulator of oxidative stress, superoxide dismutase 2 (SOD2). The efficacy of systemic delivery of SOD2 mRNA as a nanotherapeutic agent was studied in two different murine AAA models. Unbiased mass spectrometry-enabled proteomics and high-dimensional bioinformatics were used to examine pathways modulated by SOD2 overexpression.ResultsThe murine SOD2 mRNA sequence was mixed with p5RHH, an amphipathic peptide capable of delivering nucleic acidsin vivoto form self-assembled nanoparticles of ∼55 nm in diameter. We further demonstrated that the nanoparticle was stable and functional up to four weeks following self-assembly when coated with hyaluronic acid. Delivery of SOD2 mRNA mitigated the expansion of small AAA and largely prevented rupture. Mitigation of AAA was accompanied by enhanced SOD2 protein expression in aortic wall tissue. Concomitant suppression of nitric oxide, inducible nitric oxide synthase expression, and cell death was observed. Proteomic profiling of AAA tissues suggests that SOD2 overexpression augments levels of microRNAs that regulate vascular inflammation and cell apoptosis, inhibits platelet activation/aggregation, and downregulates mitogen-activated protein kinase signaling. Gene set enrichment analysis shows that SOD2 mRNA delivery is associated with activation of oxidative phosphorylation, lipid metabolism, respiratory electron transportation, and tricarboxylic acid cycle pathways.ConclusionsThese results confirm that SOD2 is key modulator of oxidative stress in AAA. This nanotherapeutic mRNA delivery approach may find translational application in the medical management of small AAA and the prevention of AAA rupture.
Single production of an exotic vectorlike
Y
quark at future high energy
pp
colliders
Authors: Shang Liangliang , Yan Yuxiao , Moretti Stefano , Yang Bingfang 
Published: June 2024

Vectorlike quarks have been predicted in various new physics scenarios beyond the Standard Model. In a simplified modelling of a (B,Y) doublet including a vectorlike quark Y, with charge −43e, there are only two free parameters: the Y coupling κY and mass mY. In the five flavor scheme, we investigate the single production of the Y state decaying into Wb at the Large Hadron Collider (LHC) run-III and High-Luminosity LHC (HL-LHC) operating at s=14  TeV, the possible High-Energy LHC (HE-LHC) with s=27  TeV as well as the Future Circular Collider in hadron-hadron mode (FCC-hh) with s=100  TeV. Through detailed signal-to-background analyses and detector simulations, we assess the exclusion capabilities of the Y state at the different colliders. We find that this can be improved significantly with increasing collision energy, especially at the HE-LHC and FCC-hh, both demonstrating an obvious advantage with respect to the HL-LHC in the case of high mY. Assuming a 10% systematic uncertainty on the background event rate, the exclusion capabilities are summarized as follows: (1) the LHC run-III can exclude the correlated regions of κY∈[0.06,0.5] and mY∈[1500  GeV,3800  GeV] with integrated luminosity L=300  fb−1; (2) the HL-LHC can exclude the correlated regions of κY∈[0.05,0.5] and mY∈[1500  GeV,3970  GeV] with L=3  ab−1; (3) the HE-LHC can exclude the correlated regions of κY∈[0.06,0.5] and mY∈[1500  GeV,6090  GeV] with L=3  ab−1; (4) the FCC-hh can exclude the correlated regions of κY∈[0.08,0.5] and mY∈[1500  GeV,10080  GeV] with L=3  ab−1.

Published by the American Physical Society
2024
A strategic process to manage the right value proposition with retailers in the B2C sector
Authors: Baidya Mehir , Maity Bipasha 
Published: June 2024

PurposeIn the past, there have been few studies that shed light on the process of how to maintain the right value proposition with retailers. This research aims to examine the factors that play a significant role in the process of keeping the right value proposition with retailers in the B2C sector through a firm-retailer dependency lens.Design/methodology/approachLongitudinal data was gathered from 700 retailers who deal with the products of two of India’s firms in the B2C sector. Three data sets were created, and an econometric model was fitted to each data set separately.FindingsThe findings revealed that the firm-centric and retailer-centric variables had positive impacts, whereas competitor-centric variables negatively impacted the value proposition. Furthermore, the intensity of the impact on the value proposition of all types of drivers varied from “quiet-quitting retailers” to “active retailers.”Practical implicationsThis study’s findings should assist managers in framing a value-sharing strategy to maintain a “win-win” relationship with retailers.Originality/valueUsing real-world data and a panel regression model, this research extends the theory on the relationship between value proposition and its drivers in the B2C sector and, hence, enriches the literature on the interface between business process management, retailing, and marketing.
Shallow strategic thinking: findings from a multinational problem-based learning pilot study
Authors: Hennessey Megan J. , Perez Celestino , Jenner Brandy 
Published: June 2024

PurposeResearchers piloted a problem-based learning (PBL) activity in a master’s degree-granting strategic studies program to explore how students apply knowledge and skills learned from the curriculum to their formulation of a strategy addressing a real-world global security scenario.Design/methodology/approachThis mixed-methods pilot study used ethnographic observation, participant feedback, document analysis and surveys to assess the learning and engagement of multinational postgraduate students in the context of a PBL environment.FindingsFindings revealed gaps in students’ causal logic and literacy, as well as student discomfort with ambiguity and reliance upon heuristic frameworks over willingness to conduct substantive, current and relevant research. Additionally, observed group dynamics represented a lack of inclusive collaboration in mixed gender and multinational teams. These findings suggest foundational issues with the curriculum, teaching methodologies and evaluation practices of the studied institution.Originality/valueThis study highlights the need to include explicit instruction in problem-solving and causal literacy (i.e. logical reasoning) in postgraduate programs for national and global security professionals, as well as authentic opportunities for those students to practice interpersonal communication.
Online VR store as a sustainable fashion retail space
Authors: Won Yujeong , Jung Hye Jung , Lee Yuri 
Published: June 2024

PurposeThe study applied the information system success model (ISSM) to investigate the influence of information system qualities (ISQs) on consumer responses related to sustainable fashion consumption in the context of comparing 3D VR store and 2D website.Design/methodology/approachThis study designed a questionnaire to measure five ISQs (usefulness, diversity, functionality, reliability and tangibility), immersion in messages and purchase intention. 270 data from women consumers of online fashion stores were collected using Macromill Embrain. Structural equation modeling with Amos 21 and process macro model 7 with SPSS 26 were used for analysis.FindingsThis study suggested the relevance of usefulness, diversity, reliability and tangibility among ISQs in evoking immersion in sustainable fashion messages and stimulating the purchase intention. In the moderation of website technology types, the effects of usefulness and tangibility on the immersion in sustainable fashion messages were stronger in 3D VR than 2D condition.Originality/valueThe study is meaningful as an initial study that identified the ISQs of online fashion stores by dividing the type of technology into 3D VR and 2D. We offer insights about the relevance and applicability of immersive VR technology in promoting sustainable fashion consumption and show the potential of online VR store as a new kind of sustainable fashion retail space.