bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a quantitative trait locus for spontaneous osteoarthritis in STR/ort mice
Authors: Watanabe Ken , Oue Yasuhiro , Miyamoto Yoshinari , Matsuura Makiko , Mizuno Yusuke , Ikegawa Shiro 
Published: June 2011

AbstractOsteoarthritis (OA) is the most common joint disorder in humans. Most of the animal models of OA were developed by surgical destabilization of joints or through transgenic approaches, and information from naturally occurring models of OA is very limited. The mouse strain STR/ort is recognized as a spontaneous model of OA. This mouse is unique in that it develops late onset cartilage degeneration of the tibio‐femoral joint, similar to human OA. The purpose of this study was to identify quantitative trait loci (QTL) for the OA phenotype in STR/ort. Whereas the trait had been reported to be recessive, a significant population of the F1 generation exhibited OA phenotype. Thus, backcrossed (BC) mice generated by crossing F1 male to C57BL/6N female mice were used for genetic analysis. Degeneration of articular cartilage in BC mice was evaluated by scanning electron microscopy. Linkage analysis was carried out using microsatellite markers covering the entire genome. Cartilage degeneration in STR/ort mice was a polygenic trait. A QTL for the OA phenotype was mapped to a region 20 centimorgans proximal to the centromere of chromosome 4 (LOD = 3.37, p = 0.0065). A QTL associated with the onset of cartilage degeneration in C57BL/6N mice was also identified on chromosome 5 (LOD = 3.04, p = 0.0147). These results suggest that multiple loci are involved in the OA phenotype in mice. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:15–20, 2012
Ultrafast Raman loss spectroscopy (URLS): instrumentation and principle
Authors: Mallick Babita , Lakhsmanna Adithya , Umapathy Siva 
Published: June 2011

AbstractIn this paper, we report on the concept and the design principle of ultrafast Raman loss spectroscopy (URLS) as a structure‐elucidating tool. URLS is an analogue of stimulated Raman scattering (SRS) but more sensitive than SRS with better signal‐to‐noise ratio. It involves the interaction of two laser sources, namely, a picosecond (ps) Raman pump pulse and a white‐light (WL) continuum, with a sample, leading to the generation of loss signals on the higher energy (blue) side with respect to the wavelength of the Raman pump unlike the gain signal observed on the lower energy (red) side in SRS. These loss signals are at least 1.5 times more intense than the SRS signals. An experimental study providing an insight into the origin of this extra intensity in URLS as compared to SRS is reported. Furthermore, the very requirement of the experimental protocol for the signal detection to be on the higher energy side by design eliminates the interference from fluorescence, which appears on the red side. Unlike CARS, URLS signals are not precluded by the non‐resonant background and, being a self‐phase‐matched process, URLS is experimentally easier. Copyright © 2011 John Wiley & Sons, Ltd.
PSCA mRNA expression in preoperatively negative prostate biopsies predicts incidental prostate cancer in patients undergoing transurethral resection of the prostate for benign prostatic hyperplasia
Authors: Zhao Zhigang , Ma Wenjing , Zeng Guohua , Qi Defeng 
Published: June 2011

AbstractBackgroundRecent data showed that prostate stem cell antigen (PSCA) mRNA expression is predictive of the subsequent cancer in BPH patients treated with TURP. This study was to determine whether PSCA mRNA expression in preoperatively negative biopsies identified the presence of incidental prostate cancer (IPCa) on TURP tissues analysis.MethodsPSCA in situ hybridization was performed in preoperatively negative prostatic biopsies taken from 592 enrolled BPH patients. Predictive performance of PSCA mRNA for IPCa was evaluated by the Cox proportional hazards models.ResultsPSCA mRNA positivity in negative biopsy specimens was detected in 84 (14.2%) of 592 men. Of those 84 patients, 57 were histopathologically identified as having PCa on their TURP tissues. None of the rest 508 men with negative PSCA mRNA expression were diagnosed with IPCa. Spearman's rank correlation coefficient showed that PSCA mRNA expression levels were correlated significantly and positively with Gleason score (r = 0.80, P < 0.001) and clinical stage (r = 0.86, P < 0.001). A multivariate Cox model demonstrated that only PSCA mRNA positivity was predictive of the IPCa presence on TURP (HR = 4.04; 95% CI: 2.12–5.42; P < 0.001), with the concordance index of 0.878.ConclusionPSCA mRNA positivity in negative biopsies is a promising marker for the presence of IPCa in BPH patients. J. Surg. Oncol. 2011; 104:672–678. © 2011 Wiley Periodicals, Inc.
How Far does Overall Service Quality of a Destination Affect Customers' Post‐Purchase Behaviours?
Authors: Moutinho Luiz , Albayrak Tahir , Caber Meltem 
Published: June 2011

ABSTRACTIn tourism management literature, there are numerous research papers focusing on the determinants of the customers' destination evaluations and how they affect each other. The chief variables to be examined in the literature have been customer satisfaction, perceived value, behavioural intention and service quality. In this study, the authors were inspired by the ‘indirect model’ by Cronin et al. (2000) and generated a conceptual model to determine the overall service quality of a destination and its effect on perceived value, customer satisfaction and behavioural intention by structural equation modelling technique. Copyright © 2011 John Wiley & Sons, Ltd.
Sub‐surface, micrometer‐scale incisions produced in rodent cortex using tightly‐focused femtosecond laser pulses
Authors: Nguyen John , Ferdman Jillian , Zhao Mingrui , Huland David , Saqqa Shatha , Ma Jan , Nishimura Nozomi , Schwartz Theodore H. , Schaffer Chris B. 
Published: June 2011

AbstractBackground and ObjectiveTechniques that allow targeted, micrometer‐scale disruption in the depths of biological tissue, without affecting overlying structures or causing significant collateral damage, could potentially lead to new surgical procedures. We describe an optical technique to make sub‐surface incisions in in vivo rodent brain and characterize the relationship between the cut width and maximum depth of these optical transections as a function of laser energy.Materials and MethodsTo produce cuts, high intensity, femtosecond laser pulses were tightly focused into and translated within the cortex, through a craniotomy, in anesthetized rodents. Imaging of stained brain slices was used to characterize cut width and maximum cutting depth.ResultsCut width decreased exponentially as a function of depth and increased as the cube root of laser energy, but showed about 50% variation at fixed depth and laser energy. For example, at a laser energy of 13 µJ, cut width decreased from 158 ± 43.1 µm (mean ± standard deviation) to 56 ± 33 µm over depths of approximately 200–800 µm, respectively. Maximal cut depth increased logarithmically with laser energy, with cut depths of up to 1 mm achieved with 13 µJ pulses. We further showcased this technique by selectively cutting sub‐surface cortical dendrites in a live, anesthetized transgenic mouse.ConclusionsFemtosecond laser pulses provide the novel capacity for precise, sub‐surface, cellular‐scale cuts for surgical applications in optically scattering tissues. Lasers Surg. Med. 43:382–391, 2011. © 2011 Wiley‐Liss, Inc.
Noninvasive laser coagulation of the human vas deferens: Optical and thermal simulations
Authors: Schweinsberger Gino R. , Cilip Christopher M. , Trammell Susan R. , Cherukuri Harish , Fried Nathaniel M. 
Published: June 2011

AbstractBackground and ObjectivesSuccessful noninvasive laser coagulation of the canine vas deferens, in vivo, has been previously reported. However, there is a significant difference between the optical properties of canine and human skin. In this study, Monte Carlo (MC) simulations of light transport through tissue and heat transfer simulations are performed to determine the feasibility of noninvasive laser vasectomy in humans.Materials and MethodsA laser wavelength of 1,064 nm was chosen for deep optical penetration in tissue. MC simulations determined the spatial distribution of absorbed photons inside the tissue layers (epidermis, dermis, and vas). The results were convolved with a 3‐mm‐diameter laser beam, and then used as the spatial heat source for the heat transfer model. A laser pulse duration of 500 milliseconds, pulse rate of 1 Hz, and cryogen spray cooling were incident on the tissue for 60 seconds. Average laser power (5–9 W), cryogen pulse duration (60–100 milliseconds), cryogen cooling rate (0.5–1.0 Hz), and increase in optical transmission due to optical clearing (0–50%) were studied.ResultsAfter application of an optical clearing agent (OCA) to increase skin transmission by 50%, an average laser power of 6 W, cryogen pulse duration of 60 milliseconds, and cryogen cooling rate of 1 Hz resulted in vas temperatures of approximately 58°C, sufficient for thermal coagulation, while 1 mm of the skin surface (epidermis and dermis) remained at a safe temperature of approximately 45°C.ConclusionsMC and heat transfer simulations indicate that it is possible to noninvasively thermally coagulate the human vas deferens without adverse effects (e.g., scrotal skin burns), if an OCA is applied to the skin prior to the procedure. Lasers Surg. Med. 43:443–449, 2011. © 2011 Wiley‐Liss, Inc.
Multi‐excitation fluorescence spectroscopy for analysis of non‐alcoholic fatty liver disease
Authors: Sauvage Vincent R. , Levene Adam P. , Nguyen Hoa T. , Wood Tobias C. , Kudo Hiromi , Concas Danilo , Thomas Howard C. , Thursz Mark R. , Goldin Robert D. , Anstee Quentin M. , Elson Daniel S. 
Published: June 2011

AbstractBackground and ObjectivesThe increasing incidence of non‐alcoholic fatty liver diseases (NAFLD) and the consequent progression to cirrhosis is expected to become a major cause of liver transplantation. This will exacerbate the organ donor shortage and mean that ‘marginal’ fatty liver grafts are more frequently used. Autofluorescence spectroscopy is a fast, objective, and non‐destructive method to detect change in the endogenous fluorophores distribution and could prove to be a valuable tool for NAFLD diagnosis and transplant graft assessment.Materials and MethodsA system was constructed consisting of a fibre probe with two laser diodes that provided excitation light at 375 and 405 nm, and an imaging spectrograph system. This was used to distinguish fluorescence spectra acquired from the harvested livers from mice with NAFLD of differing severity (healthy, mild steatotic and steatohepatitic). The fluorescence data were entered into a sparse multiclass probabilistic algorithm for disease classification. Histopathology, thiobarbituric acid reactive substances (TBARS) and alanine transaminase (ALT) assays were conducted in addition to the fluorescence measurementsResultsTBARS and ALT assays enabled differentiation of the steatohepatitic group from the mild steatosis and control groups (P ≤ 0.028) but failed to separate the mild steatotic group from the control group. The three groups were all clearly differentiated from each other using fluorescence spectroscopy, and classification accuracy was found to be 95%.ConclusionFluorescence spectroscopy appears to be a promising approach for the analysis of diseased liver tissue. Lasers Surg. Med. 43:392–400, 2011. © 2011 Wiley‐Liss, Inc.
Photochemical tissue bonding: A potential strategy for treating limbal stem cell deficiency
Authors: Gu Chuan , Ni Tao , Verter Erol E. , Redmond Robert W. , Kochevar Irene E. , Yao Min 
Published: June 2011

AbstractBackground and ObjectiveTo determine the feasibility of attaching human amniotic membrane (HAM), pre‐cultured with limbal stem cells (LSCs), to cornea using a novel, light‐activated tissue bonding method.Materials and MethodsLSCs were isolated from rabbit eyes, and then cultured on de‐epithelialized HAM to create grafts (HAM/LSC). These were then transplanted onto rabbit eyes with surgically created limbal stem cell deficiency (LSCD). The grafts were secured either by sutures or by a light‐activated method called photochemical tissue bonding (PTB). Outcomes included corneal opacity, inflammation, neovascularization, and collagen alignment.ResultsThe isolated and cultured cells were verified to be LSCs based on their K19+/intergrin β1+/P63+/K3 profile. Securing the HAM/LSC graft with PTB provided better outcomes. At 28 days post‐surgery, the corneal opacity scores were significantly lower after securing the graft with PTB compared with suture attachment (0.8 ± 0.5 vs. 1.8 ± 0.5, P < 0.01). Similarly, neovascularization scores were lower after PTB (0.8 ± 0.5 vs. 1.5 ± 0.6, P < 0.01). Quantification of MPO and CD31 levels from immunofluorecent staining indicated that PTB stimulated less neutrophil infiltration (5.3 ± 2.2 vs. 13.3 ± 3.1, P < 0.01) and less new blood vessels formation (2.0 ± 0.8 vs. 6.3 ± 1.3, P < 0.01) at the wound site. The collagen alignment in PTB‐treated corneas, as shown by immunofluorescence and second harmonic generation image, was better organized in the PTB‐treated group than in the suture group.ConclusionBonding LSC grafts with PTB produced improved outcomes compared to suture attachment. This light‐activated method is a promising modality for treating patients with LSCD. Lasers Surg. Med. 43:433–442, 2011. © 2011 Wiley‐Liss, Inc.
Existence and uniqueness for the p(x)‐Laplacian‐Dirichlet problems
Authors: Fan Xianling 
Published: June 2011

AbstractTwo results on the existence and uniqueness for the p(x)‐Laplacian‐Dirichlet problem −div(|∇u|p(x) − 2∇u) = f(x, u) in Ω, u = 0 on ∂Ω, are obtained. The first one deals with the case that f(x, u) is nonincreasing in u. The second one deals with the radial case in which f(r, u) is nondecreasing in u and satisfies the sub‐p− − 1 growth condition. © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Nimbolide retards tumor cell migration, invasion, and angiogenesis by downregulating MMP‐2/9 expression via inhibiting ERK1/2 and reducing DNA‐binding activity of NF‐κB in colon cancer cells
Authors: Babykutty Suboj , P.S. Priya , R.J. Nandini , Kumar M.A. Suresh , Nair Mangalam S. , Srinivas Priya , Gopala Srinivas 
Published: June 2011

AbstractNimbolide, a plant‐derived limonoid has been shown to exert its antiproliferative effects in various cell lines. We demonstrate that nimbolide effectively inhibited proliferation of WiDr colon cancer cells through inhibition of cyclin A leading to S phase arrest. It also caused activation of caspase‐mediated apoptosis through the inhibition of ERK1/2 and activation of p38 and JNK1/2. Further nimbolide effectively retarded tumor cell migration and invasion through inhibition of metalloproteinase‐2/9 (MMP‐2/9) expression, both at the mRNA and protein level. It was also a strong inhibitor of VEGF expression, promoter activity, and in vitro angiogenesis. Finally, nimbolide suppressed the nuclear translocation of p65/p50 and DNA binding of NF‐κB, which is an important transcription factor for controlling MMP‐2/9 and VEGF gene expression. © 2011 Wiley Periodicals, Inc.
Generalized double‐acquisition imaging for radiofrequency inhomogeneity mitigation in high‐field MRI: Experimental proof and performance analysis
Authors: Ferrand Guillaume , Luong Michel , Amadon Alexis , Cloos Martijn A. , Giacomini Eric , Darrasse Luc 
Published: June 2011

AbstractTransmit arrays have been developed to compensate for radiofrequency inhomogeneities in high‐field MRI using different excitation schemes. They can be classified into static or dynamic shimmings depending on the target: homogenizing the radiofrequency field directly or homogenizing the flip angle distribution using the Bloch equation. We have developed an intermediate solution to compare shimming performances between different transmit arrays. This solution, called generalized double‐acquisition imaging, is easier to implement than most dynamic shimming methods and offers more degrees of freedom than static shimmings. It uses two acquisitions so that the second acquisition complements the excitation of the first one to obtain by superposition an image that minimizes radiofrequency artefacts. For validation, the method is demonstrated experimentally for a gradient echo sequence on a spherical homogeneous phantom and by simulation on a human head model. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Restricted or severely hindered diffusion of intramyocellular lipids in human skeletal muscle shown by in vivo proton MR spectroscopy
Authors: Brandejsky Vaclav , Kreis Roland , Boesch Chris 
Published: June 2011

AbstractAlthough magnetic resonance spectroscopy can be used as a unique tool to study molecular diffusion, it is rarely used to measure the diffusion properties of intramyocellular and extramyocellular lipids. Lipids have very low apparent diffusion coefficients (ADCs), which make these measurements difficult and necessitate strong diffusion gradients and long diffusion times. Consequence is that these measurements have inherently low signal‐to‐noise ratio and are prone to artifacts. The addition of physiological triggering and individual storage and processing of the spectra is seen to be a possible approach to maximize signal intensity and achieve high reproducibility of the experiments. Thus, the optimized measurement protocol was used to investigate the diffusion properties of lipids in human skeletal muscle in vivo. At a diffusion time of about 110 ms, intramyocellular lipids show a significantly lower ADC (2.0 × 10−6 mm2/s, 95% confidence interval 1.10 × 10−6 to 2.94 × 10−6 mm2/s) than extramyocellular lipids (1.58 × 10−5 mm2/s, 95% confidence interval 1.41 × 10−5 to 1.75 × 10−5 mm2/s). Because the chemical properties of both lipid pools can be assumed to be similar, the difference can only be attributed to restricted or severely hindered diffusion in the intramyocellular droplets. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.
The functional microstructure of tendon collagen revealed by high‐field MRI
Authors: Mountain Kelsey M. , Bjarnason Thorarin A. , Dunn Jeff F. , Matyas John R. 
Published: June 2011

AbstractT2 was used in this study to assess tendon microstructure. Two unloaded digital extensor tendons were bent such that their long axes were imaged throughout 180° with respect to B0. T2‐weighted images reveal periodic banding (∼200 μm) when tendons were oriented at ±55° with respect to B0. Five pairs of tendons were used to study the influence of load on T2W MRI: one tendon of each pair was loaded with a 7.8‐N mass, and both tendons were fixed in formalin then imaged at 55° to B0. MRI banding was present in the unloaded, but not loaded, tendons. In unloaded tendons, polarized‐light microscopy revealed collagen crimp with a periodicity similar to MRI. In loaded tendons, there was a strain‐induced extinction of periodicity on both MRI and polarized‐light microscopy. These studies confirm that crimp is detectable by high‐field MRI and could serve as an in vivo index of physiological strains in collagenous tissues. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
An interactive voice response diary for patients with non‐dystrophic myotonia
Authors: Statland Jeffrey M. , Wang Yunxia , Richesson Rachel , Bundy Brian , Herbelin Laura , Gomes Joe , Trivedi Jaya , Venance Shannon , Amato Anthony , Hanna Michael , Griggs Robert , Barohn Richard J. ,   The Cinch Consortium
Published: June 2011

AbstractIntroduction: Non‐dystrophic myotonia (NDM) is caused by mutations in muscle chloride and sodium channels. Currently, there is no standardized instrument for documenting symptom frequency and severity in NDM. Methods: Subjects used an automated, interactive, telephone‐based voice response diary (IVR) to record frequency and severity of stiffness, weakness, pain, and tiredness once a week for 8 weeks, after their baseline visits. Results: We describe the IVR and report data on 76 subjects for a total of 385 person‐weeks. Overall there were 5.1 calls per subject. Forty‐eight subjects called in 5 or more times, and 14 called in 8 times. Stiffness was both the most frequent and severe symptom. Warm‐up and handgrip myotonia were associated with higher severity scores for stiffness. Conclusions: IVR is a convenient technology to allow patient reporting of repeated and real‐time symptom frequency and severity, and it is presently being used in a trial of mexiletine in NDM. Muscle Nerve 44: 30–35, 2011
Reinnervation by the contralateral facial nerve in patients with peripheral facial palsy
Authors: Casanova‐Molla Jordi , León Luci , Castillo Carlos David , Valls‐Solé Josep 
Published: June 2011

AbstractIntroduction: Reinnervation activity is triggered after complete unilateral peripheral facial palsy (PFP). Methods: In 27 patients with PFP we recorded electromyographic activity with a concentric needle electrode inserted 1 cm lateral to the oral commissure of the affected side. We applied electrical stimuli to the unaffected (contralateral) facial nerve from the tragus to the mid‐lower lip and measured the response latency variability and segmental conduction velocity. Results: Responses to electrical stimulation of the unaffected facial nerve were found in all patients. Mean conduction velocity was 49.6 ± 6.2 m/s between tragus and oral commissure, and 6.0 ± 1.9 m/s between oral commissure and mid‐lower lip. Latency variability was 0.27 ms to facial nerve stimulation and 0.08 ms to oral commissure stimulation. Conclusion: Short distance sprouting of axons that innervate muscle fibers, which originate from the unaffected facial nerve, results in propagation of impulses to muscle fibers in the midline. Muscle Nerve 2011
Repeated botulinum‐a toxin injection in the treatment of neuropathic bladder dysfunction and poor bladder compliance in children with myelomeningocele
Authors: Horst Maya , Weber Daniel M. , Bodmer Christine , Gobet Rita 
Published: June 2011

AbstractAimsTo evaluate the efficacy of intradetrusor injection of Botulinum‐A toxin (BoNT/A) in children with neuropathic bladder dysfunction and poor bladder compliance, due to meningomyelocele.MethodsWe retrospectively reviewed the charts of 11 children (mean age 6.7 ± 5.3 years) with neuropathic bladder and poor bladder compliance (mean 7 ml/cmH2O) treated with intradetrusor injection of BoNT/A. After urodynamic evaluation a standardized injection was performed. The authors describe their results in 11 children after the first (5×) or repeated (6×) BoNT/A injections. Re‐evaluation was performed 3 and 12 months after each injection.ResultsThree months after injection detrusor pressure decreased by 17% and bladder capacity increased by 33%. In all but 1 patient bladder compliance stayed poor. Twelve months after treatment patients still had an effect but the values were already reverting towards preoperative levels. With repeated injections we achieved a similar effect on capacity and detrusor pressure as after the first injection. Overall, bladder compliance normalized (>20 cmH2O) in only 1 patient.ConclusionsThe results confirmed the positive temporary effect of BoNT/A on detrusor pressure and bladder capacity in children with myelomeningocele, even after repeated injections. Nevertheless, bladder compliance stayed severely pathological. Whether an earlier treatment may prevent poor bladder compliance is a yet unanswered question. Neurourol. Urodynam. 30: 1546–1549, 2011. © 2011 Wiley Periodicals, Inc.