bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preference-Based Quality of Life of End-Stage Ankle Arthritis Treated with Arthroplasty or Arthrodesis
Authors: Slobogean Gerard P. , Younger Alastair , Apostle Kelly L. , Marra Carlo A. , Wing Kevin , Penner Murray , Daniels Tim , Glazebrook Mark 
Published: July 2010

Background: Health state values, or “utilities,” are an important preference-based measure of quality of life used by health economists. This study describes the utilities reported by a multicenter cohort of subjects with end-stage ankle arthritis treated with ankle arthrodesis or total ankle arthroplasty. Materials and Methods: Subjects with end-stage ankle arthritis were enrolled in a multicenter prospective cohort study. All subjects received either ankle arthrodesis or total ankle arthroplasty. Participants completed baseline SF-36 outcome evaluations preoperatively and at 1-year followup. Preference-based quality of life was assessed using health state values (HSVs) derived from the SF-36 (SF-6D transformation). Results: 107 subjects were included. The mean baseline SF-6D health state value for the TAA group was 0.67 (95% CI 0.64 to 0.69) and 0.66 (95% CI 0.63 to 0.68) for the arthrodesis group. At 1-year followup, the mean reported health state value was 0.73 (95% CI 0.71 to 0.76) for the total ankle arthroplasty group and 0.73 (95% CI 0.70 to 0.76) for the ankle arthrodesis group. The 1-year followup results approach age- and gender-matched US population norms. Health state values poorly correlated with age, however, significant differences between genders were detected. Conclusion: These data demonstrate an improvement in preference-based quality of life following ankle arthroplasty or arthrodesis. The results also provide necessary data that can be used in future cost-effectiveness analyses. Level of Evidence: II, Prospective Comparative Study
Modified Plantar Plate Tenodesis for Correction of Claw Toe Deformity
Authors: Lui T. H. , Chan L. K. , Chan K. B. 
Published: July 2010

Background: The purpose of the study was to evaluate the efficacy and safety of the modified plantar plate tenodesis for correction of claw toe deformity. Materials and Methods: Modified plantar plate tenodesis was performed in ten fresh frozen cadaveric feet with claw toe deformity of the second toe. The plantar plate of the second metatarsophalangeal joint was anchored to the extensor digitorum longus tendon by a figure-of-eight suture. The figure-of-eight construct and the relationship of the digital nerve and the suture were studied. A clinical study was conducted to study the operative times, degree of correction and the improvement of AOFAS score between groups with the original plantar plate tenodesis (Group 1) and modified technique (Group 2). Any neural injury if present was also noted. Result: In the cadaveric study, the claw toe deformity was corrected and no nerve injury occurred in any specimen. There was flexor tendon tethering by the suture in 2 specimens. In the clinical study, the average operative time was 51 minutes for Group 1 and 31 minutes for Group 2. The improvement in AOFAS score averaged 44 in Group 1 and 43 in Group 2. The corrective power of the sagittal plane deformity at the metatarsophalangeal joint averaged 25 degrees in Group 1 and 23 degrees in Group 2. Conclusion: Plantar plate tenodesis was effective in correction of flexible claw second toe deformity by stabilization of the attenuated plantar plate. The modified technique allowed easier retrieval of the suture and shorter operative time. However, it may result in tethering of the flexor tendon. Level of Evidence: III, Retrospective, Case Control Therapeutic Study
Ankle Block Implemented Through Two Skin Punctures
Authors: Hromádka Rastislav , Barták Vladislav , Popelka Stanislav , Pokorný David , Jahoda David , Sosna Antonín 
Published: July 2010

Background: Recently, peripheral nerve blocks have increasingly been used in orthopedic surgery. The foot block is an alternative for anesthesia in cases of forefoot and midfoot operations. We propose a modification of the block technique due to potential difficulties concerning the tibial nerve. Materials and Methods: The spatial position of the tibial nerve in the neurovascular bundle, proximal to entering the tarsal tunnel and sural nerve behind lateral malleolus was measured on 60 dissected preparations. Modification of the block technique was proposed. A tibial nerve block was administered by inserting a needle, at an area above the upper edge of the heel bone, tangential to the Achilles tendon. The needle was then withdrawn and redirected to the frontal plane and inserted through the tissue, anterior to the Achilles tendon and laterally behind the lateral malleolus to block the sural nerve. A block of the saphenous nerve superficial and deep peroneal nerves was implemented by needle insertion subcutaneously two centimeters proximal to the crest of the ankle joint. The technique was then evaluated in the clinical part of the study in 84 operative procedures. Results: The tibial nerve is located 21.1 mm ± 2.1 mm from the medial aspect of the Achilles tendon and 11.6 mm ± 1.3 mm deep in the neurovascular bundle. The distance from the posterior margin of the lateral malleolus to the sural nerve is 18.3 mm ± 1.9 mm. We achieved a 93% success rate in implementation of the complete foot block in 84 operations. Conclusion: The technique, proposed in the anatomical portion of the study and evaluated in the clinical part, had a similar success rate when compared to techniques published in the literature. Though comparable to currently used techniques, this technique provides easier positioning of a patient and a complete block of the foot can be done with two skin injection sites. Level of Evidence: IV, Case Series
An Atopic Dermatitis-Like Skin Disease with Hyper-IgE-emia Develops in Mice Carrying a Spontaneous Recessive Point Mutation in theTraf3ip2(Act1/CIKS) Gene
Authors: Matsushima Yoshibumi , Kikkawa Yoshiaki , Takada Toyoyuki , Matsuoka Kunie , Seki Yuta , Yoshida Hisahiro , Minegishi Yoshiyuki , Karasuyama Hajime , Yonekawa Hiromichi 
Published: July 2010

AbstractSpontaneous mutant mice that showed high levels of serum IgE and an atopic dermatitis (AD)-like skin disease were found in a colony of the KOR inbred strain that was derived from Japanese wild mice. No segregation was observed between hyper-IgE-emia and dermatitis in (BALB/c × KOR mutant) N2 mice, suggesting that the mutation can be attributed to a single recessive locus, which we designated adjm (atopic dermatitis from Japanese mice). All four adjm congenic strains in different genetic backgrounds showed both hyper-IgE-emia and dermatitis, although the disease severity varied among strains. Linkage analysis using (BALB/c × KOR-adjm/adjm) N2 mice restricted the potential adjm locus to the 940 kb between D10Stm216 and D10Stm238 on chromosome 10. Sequence analysis of genes located in this region revealed that the gene AI429613, which encodes the mouse homologue of the human TNFR-associated factor 3-interacting protein 2 (TRAF3IP2) protein (formerly known as NF-κB activator 1/connection to IκB kinase and stress-activated protein kinase/Jun kinase), carried a single point mutation leading to the substitution of a stop codon for glutamine at amino acid position 214. TRAF3IP2 has been shown to function as an adaptor protein in signaling pathways mediated by the TNFR superfamily members CD40 and B cell-activating factor in epithelial cells and B cells as well as in the IL-17–mediated signaling pathway. Our results suggest that malfunction of the TRAF3IP2 protein causes hyper-IgE-emia through the CD40- and B cell-activating factor-mediated pathway in B cells and causes skin inflammation through the IL-17–mediated pathway. This study demonstrates that the TRAF3IP2 protein plays an important role in AD and suggests the protein as a therapeutic target to treat AD.
IgE Transcripts in the Circulation of Allergic Children Reflect a Classical Antigen-Driven B Cell Response and Not a Superantigen-Like Activation
Authors: Kerzel Sebastian , Rogosch Tobias , Struecker Benjamin , Maier Rolf F. , Zemlin Michael 
Published: July 2010

Abstract
Allergic asthma is the most frequent chronic disorder in childhood. Although IgE is a central effector molecule in allergic diseases, the nature of the IgE response is still under debate. The objective of our study was to clarify whether the IgE repertoire in the circulation of allergic children represents a classical Ag-driven and oligoclonal B cell response, a superantigen-like activation of a subset of B cells, or a polyclonal B-1 cell expansion. Using a highly sensitive RT-PCR method, we amplified, cloned, and sequenced IgE H chain transcripts from 13 children with allergic asthma. We gained 1366 functional IgE sequences, which currently represent the most extensive collection of human IgE transcripts. Compared to IgM transcripts from the same children, the somatic mutation rate was significantly enhanced in IgE transcripts (21‰ versus 72‰; p &lt; 0.001), which renders a polyclonal B-1 response unlikely. Moreover, IgE sequences displayed significantly enhanced Ag selection and hence were indicative of a classical Ag-driven immune response with affinity maturation (p &lt; 0.001). In contrast to several recent studies, the usage pattern of variable gene segment of the H Ig chain in IgE transcripts followed the germline complexity, arguing against a superantigen-like interaction. We conclude that IgE transcripts in the circulation of children with allergic asthma reflect a classical adaptive B-2 cell response. This study provides reference data for a better characterization of the IgE response under immunomodulating therapies, such as anti-IgE therapy or allergen-specific immunotherapy.
XBP-1 Couples Endoplasmic Reticulum Stress to Augmented IFN-β Induction via a cis-Acting Enhancer in Macrophages
Authors: Zeng Ling , Liu Yi-Ping , Sha Haibo , Chen Hui , Qi Ling , Smith Judith A. 
Published: July 2010

Abstract
Perturbation of the endoplasmic reticulum (ER) results in a conserved stress response called the unfolded protein response (UPR). Macrophages undergoing a UPR respond to LPS with log-fold increased production of IFN-β, a cytokine with diverse roles in innate and adaptive immunity. In this study, we found that thapsigargin-induced ER stress augmented recruitment of IFN regulatory factor-3, CREB binding protein/p300, and transcriptional machinery to the murine ifnb1 promoter during LPS stimulation. Although full synergistic IFN-β production requires X-box binding protein 1 (XBP-1), this UPR-regulated transcription factor did not appreciably bind the ifnb1 promoter. However, XBP-1 bound a conserved site 6.1 kb downstream of ifnb1, along with IFN regulatory factor-3 and CREB binding protein only during concomitant UPR and LPS stimulation. XBP-1 physically associates with p300, suggesting a mechanism of multimolecular assembly at the +6.1 kb site. Luciferase reporter assays provide evidence this +6 kb region functions as an XBP-1–dependent enhancer of ifnb1 promoter activity. Thus, this study identifies a novel role for a UPR-dependent transcription factor in the regulation of an inflammatory cytokine. Our findings have broader mechanistic implications for the pathogenesis of diseases involving ER stress and type I IFN, including viral infection, ischemia-reperfusion injury, protein misfolding, and inflammatory diseases.
An Allelic Variant of Crry in the Murine Sle1c Lupus Susceptibility Interval Is Not Impaired in Its Ability To Regulate Complement Activation
Authors: Tchepeleva Svetlana N. , Thurman Joshua M. , Ruff Katherine , Perkins Stephen J. , Morel Laurence , Boackle Susan A. 
Published: July 2010

Abstract
The Sle1c subinterval on distal murine chromosome 1 confers loss of tolerance to chromatin. Cr2, which encodes complement receptors 1 and 2 (CR1/CR2; CD35/CD21), is a strong candidate gene for lupus susceptibility within this interval based on structural and functional alterations in its protein products. CR1-related protein/gene Y (Crry) lies 10 kb from Cr2 and encodes a ubiquitously expressed complement regulatory protein that could also play a role in the pathogenesis of systemic lupus erythematosus. Crry derived from B6.Sle1c congenic mice migrated at a higher m.w. by SDS-PAGE compared with B6 Crry, as a result of differential glycosylation. A single-nucleotide polymorphism in the first short consensus repeat of Sle1c Crry introduced a novel N-linked glycosylation site likely responsible for this structural alteration. Five additional single-nucleotide polymorphisms in the signal peptide and short consensus repeat 1 of Sle1c Crry were identified. However, the cellular expression of B6 and B6.Sle1c Crry and their ability to regulate the classical pathway of complement were not significantly different. Although soluble Sle1c Crry regulated the alternative pathway of complement more efficiently than B6 Crry, as a membrane protein, it regulated the alternative pathway equivalently to B6 Crry. These data fail to provide evidence for a functional effect of the structural alterations in Sle1c Crry and suggest that the role of Cr2 in the Sle1c autoimmune phenotypes can be isolated in recombinant congenic mice containing both genes.
Qualidade de vida de cuidadores primários de crianças com paralisia cerebral: revisão da literatura
Authors: Prudente Cejane Oliveira Martins , Barbosa Maria Alves , Porto Celmo Celeno 
Published: July 2010

O nascimento de uma criança com Paralisia Cerebral provoca mudanças na instituição familiar. Desta forma, os objetivos deste estudo foram analisar a qualidade de vida dos cuidadores primários de crianças com Paralisia Cerebral e correlacionar a qualidade de vida dos cuidadores primários de crianças com Paralisia Cerebral com o nível de comprometimento motor das crianças. Tratou-se de uma revisão da literatura, junto às bases de dados LILACS, MEDLINE, SciELO e PubMed, com os descritores qualidade de vida, cuidadores, mães, pais e paralisia cerebral, onde foram utilizados artigos científicos publicados entre 1995 a 2009. Dos 36 artigos encontrados, 7 fizeram parte da amostra. Os artigos analisados mostraram que pelo menos alguns aspectos da qualidade de vida dos cuidadores primários de crianças com Paralisia Cerebral são de modo geral pior que o dos cuidadores primários de crianças saudáveis. Não há, contudo, consenso entre os autores que correlacione qualidade de vida dos cuidadores com o nível de comprometimento motor das crianças com Paralisia Cerebral, mas os estudos analisados tiveram limitações na amostra, não sendo constituídos por grupos homogêneos, segundo o Sistema de Classificação da Função Motora. Conclui-se que os profissionais da saúde devem direcionar seu olhar aos cuidadores de crianças com Paralisia Cerebral. Descritores: Qualidade de vida; Mães; Cuidadores; Paralisia Cerebral.
Cuidados ao recém nascido em fototerapia: o conhecimento da equipe de enfermagem
Authors: Gomes Nathália Silva , Teixeira Jesislei Bonolo do Amaral , Barichello Elizabeth 
Published: July 2010

A icterícia é uma das alterações mais frequentes tanto em recém-nascidos a termo quanto em prematuros. A fototerapia é o tratamento mais utilizado nestes casos, porém este tratamento pode ocasionar algumas alterações no bebê, as quais devem ser prevenidas e detectadas precocemente pela equipe de enfermagem. O objetivo do estudo foi identificar o conhecimento da equipe de enfermagem acerca dos cuidados ao recém-nascido e complicações relacionadas a fototerapia. Trata-se de um estudo prospectivo e descritivo realizado com a equipe de enfermagem dos setores de Serviço de enfermagem da Unidade Neonatológica, Alojamento Conjunto e CTI Neonatal de um Hospital Universitário. As temáticas da entrevista abordaram sobre: quando é iniciada a fototerapia no RN, quem realiza a avaliação da evolução da icterícia, quais os possíveis efeitos colaterais no RN e quem os avalia, e sobre os cuidados realizados ao RN em fototerapia e aos equipamentos utilizados. Percebemos que o melhor conhecimento da equipe de enfermagem sobre os cuidados relacionados ao recém-nascido em fototerapia e aos aparelhos utilizados propicia maior qualidade na assistência, resultados mais rápidos e eficazes e reconhecimento do trabalho da enfermagem no cuidado. Descritores: Icterícia; Fototerapia; Enfermagem.
Creatine transporter deficiency in two half‐brothers
Authors: Ardon Orly , Filippo Cristina Amat di San , Salomons Gajja S. , Longo Nicola 
Published: July 2010

AbstractX‐linked cerebral creatine deficiency is caused by the deficiency of the creatine transporter encoded by the SLC6A8 gene. Here, we report two half‐brothers with this condition and characterize creatine transport in human fibroblasts. The propositus presented at 6 months of age with delays in development and slow progress since then with no regression. Seizures started at 3.5 years of age and responded well to treatment with anticonvulsants. He had failure to thrive with all growth parameters (including head size) at or below the fifth centile. Brain MRI indicated hemispheric white matter abnormalities, while MR spectroscopy indicated markedly reduced creatine peak. Biochemical testing indicated increased urine creatine/creatinine ratio, with normal plasma creatine and guanidinoacetate. To confirm the diagnosis, we measured [14]C‐creatine transport in fibroblasts. [14]C‐Creatine transport in normal human fibroblasts was linear for up to 2 hr at 37°C. Kinetic studies indicated the presence of a single saturable creatine transporter with a Km of 34.7 ± 2.5 µM. Fibroblasts from the propositus lacked creatine transport. DNA testing indicated hemizygosity for a novel deletion producing a frameshift (c.974_975delCA, p.Thr325SerfsX139) in the creatine transporter gene. His 12‐year‐old half‐brother had similar biochemical and clinical abnormalities except for the presence of macrocephaly and the absence of seizures. The mother had history of seizures in childhood, but had normal development. These results show that human fibroblasts have a single major creatine transporter and that measurement of its specific activity can confirm creatine transporter deficiency. © 2010 Wiley‐Liss, Inc.
Synthesis of acetophenone formaldehyde resin containing ABA type block copolymers by ATRP
Authors: Canak Tuba Cakır , Kızılcan Nilgün , Serhatlı İ. Ersin 
Published: July 2010

AbstractWell‐defined ABA type block copolymers of acetophenone formaldehyde resin (AFR) and methyl methacrylate (MMA) were synthesized via atom transfer radical polymerization. In the first step, acetophenone formaldehyde resin containing hydroxyl groups was modified with 2‐bromopropionyl bromide. Resulting difunctional macroinitiator was used in the ATRP of MMA using copper bromide (CuBr)/N,N,N′,N″,N″‐pentamethyl‐diethylenetriamine (PMDETA) as the catalyst system at 90°C. The chemical composition and structure of the copolymers were characterized by nuclear magnetic resonance (1H‐NMR) spectroscopy, Fourier transform infrared (FT‐IR) spectroscopy, and molecular weight measurement. Gel permeation chromatography (GPC) was used to study the molecular weight distributions of the AFR block copolymers. Mn up to 24,000 associated with narrow molecular weight distributions (PDI < 1.5) were obtained with conversions up to 79%. Coating properties of obtained block copolymers such as adhesion and reflectance values were investigated. They showed good adhesion properties on Plexiglass substrates. Reflectance values increased as the resin content of polymer increased. The thermal properties of all polymers were studied using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). All block copolymers showed higher thermal stability than their precursor AFR resin. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
PE/OMMT nanocomposites catalyzed by the OMMT‐intercalated Et[Ind]2ZrCl2 in slurry polymerization: Effects of organic solvent on ethylene polymerization behaviors and the nanocomposite structures
Authors: Huang Ying‐Juan , Qin Ya‐Wei , Dong Jin‐Yong 
Published: July 2010

AbstractIn situ intercalative polymerization for ethylene monomers was carried out to produce PE‐based hybrids through a slurry polymerization method. In this approach, organic solvent for olefin polymerization was found to be one of the most significant factors for the dispersion of the OMMT‐intercalated Et[Ind]2ZrCl2 catalysts, which determines that whether olefin monomers polymerize is in a well‐defined confinement environment or not. Understanding the olefin polymerization occurring in between the nanoscale silicate layers of OMMT as well as the corresponding structure of OMMT in an organic polymerization solvent is a critical step toward tailoring and characterizing nanocomposites formed by OMMT in a polyolefin matrix. As we know, the Et[Ind]2ZrCl2 catalyst and MAO are both better dissolved in toluene than that in hexane because of the larger polarity of toluene. Thus, in hexane the active sites of the OMMT/Et[Ind]2ZrCl2 catalyst exist in the silicate layers of OMMT and the PE chains grow in the middle of them, while in toluene the active specimens are exposed in the gel formed by the OMMT‐intercalated catalyst with MAO, which cause that the PE chains propagated in the mixture liquids. Consequently, when hexane is selected as the polymerization solvent, the formed PE‐based nanocomposites have a good dispersion of OMMT and the nanofiller content (TGA measurement residue at 600°C) is thus higher (>7.0 wt %). However, in toluene, most of the silicate layers of OMMT are agglomerated in the PE matrix. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Influence of thermal ageing on surface degradation of ethylene‐propylene‐diene elastomer
Authors: Nevshupa R. , Martínez L. , Álvarez L. , López M. F. , Huttel Y. , Méndez J. , Román E. 
Published: July 2010

AbstractEvolution of the surface characteristics of EPDM elastomer was studied as a function of thermal ageing at 80°C and 120°C in air for up to 100 days. The components of the surface free energy (SFE) were determined from contact angle measurements of five liquids using acid–base regression and Fowke's theories. SFE increased with ageing duration. For ageing at 80°C, SFE increased exponentially with time and stabilized after 60 days. Ageing at 120°C promoted a steep enhancement of SFE within the first 5 days followed by a slow long‐term linear increase. Rate parameters of the SFE evolution were analyzed using Dakin's kinetic model. Analysis of the surface composition using X‐ray photoemission spectroscopy evidenced an enhancement of the oxygen content with increasing the ageing time. The increase of the polar component of the SFE was attributed to the presence of these oxygen functional groups. This tendency was more pronounced for ageing at 80°C. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011