bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zika virus-induced fetal demise is driven by strain- and dose-specific RLR-driven activation of the interferon response in the decidua, placenta, and fetus inIfnar1-/-mice
Authors: Bohm Ellie K. , Castañeda David , Lu Qun , Cameron Michael D. , Aliota Matthew T. 
Published: February 2025

ABSTRACTCongenital Zika syndrome (CZS), the set of fetal and neonatal complications associated with Zika virus (ZIKV) infection in pregnancy, was first noted during the outbreak in the Americas in 2015-16. However, there was an unequal distribution of ZIKV cases and severe outcomes in all areas where ZIKV emerged in the Americas, demonstrating that the risk of CZS varied over space and time. Recently, we demonstrated that phenotypic heterogeneity existed between closely-related ZIKV strains. All ZIKV strains tested infected the placenta but varied in their capacity to cause overt fetal harm. Here, we further characterized the relative contributions of virus genotype and infecting dose of two phenotypically distinct ZIKV strains across multiple timepoints in gestation in pregnant mice that lack type-I interferon receptor function (Ifnar1-/-). To better understand the underlying causes of adverse fetal outcomes, we used RNA sequencing to compare ZIKV-infected and uninfected tissues. We found that ZIKV infection triggers retinoic acid-inducible gene I (RIG-I)-like receptor-mediated activation of the interferon response at the maternal-fetal interface. However, modest chemical inhibition of RIG-I activation in the decidua and placenta did not protect against fetal demise. Instead, the fetal interferon response was significantly associated with fetal demise. Together, these findings suggest that the response to ZIKV at the maternal-fetal interface can vary depending on the infecting ZIKV genotype and dose, and that the fetal immune response is an important mediator of fetal harm.IMPORTANCEPreviously, we used a mouse model of ZIKV infection during pregnancy to assess the pathogenic potential to the fetus of a panel of five, low-passage ZIKV strains representing the viral genetic diversity in the Americas. We found that phenotypic heterogeneity existed between these closely-related ZIKV strains. Here, we show that this heterogeneity is driven by retinoic acid-inducible gene I (RIG-I)-like receptor-mediated activation of the interferon response at the maternal-fetal interface. We used chemical inhibition of the RIG-I pathway and measured the transcriptional activity of interferon stimulated genes in fetuses to demonstrate that the fetal immune response may contribute to fetal demise.
Modeling dynamic oxygen permeability as a mechanism to mitigate oxygen-induced stresses on photosynthesis and N2fixation in marineTrichodesmium
Authors: Luo Weicheng , Inomura Keisuke , Prášil Ondřej , Eichner Meri , Luo Ya-Wei 
Published: February 2025

ABSTRACTTrichodesmium, the predominant marine diazotrophic cyanobacterium, concurrently performs nitrogen (N2) fixation and photosynthesis, the latter of which produces oxygen (O2) that inhibits N2fixation. Hopanoid lipids inTrichodesmiummay play a role in dynamically regulating membrane permeability to O2, potentially alleviating O2stress on N2fixation. However, the physiological impacts of this dynamic permeability are not well understood. We developed a model showing that dynamically modulating membrane O2permeability can enhance N2fixation and growth ofTrichodesmiumby over 50%. High O2permeability (1.5×10-4of O2diffusivity in seawater) during strong photosynthesis accelerates O2exhaust, reducing energy-consuming photorespiration by ∼40%, while low O2permeability (1.0×10-5diffusivity) during active N2fixation minimizes O2stress on N2fixation. Together, these mechanisms increase the carbon and iron use efficiencies by ∼70%. Our study provides a mechanistic and quantitative framework for how dynamic O2permeability benefitsTrichodesmium, offering insights potentially applicable to other diazotrophs.IMPORTANCETrichodesmiumis a key player in marine N2fixation, essential for oceanic productivity and global biogeochemical cycles. However, a significant challenge arises from the concurrent photosynthetic production of O2during N2fixation, which can inhibit N2fixation and cause energy-wasting photorespiration. We develop a physiological model showing thatTrichodesmiummay dynamically regulate membrane O2permeability to enhance N2fixation and growth. The model suggests two mechanisms: elevated O2permeability during the early daytime of strong photosynthesis accelerates O2exhaust to environment, reducing photorespiration, while reduced O2permeability later limits O2influx from environment, lowering wasteful respiration and maintaining a low intracellular O2level for active N2fixation. These adaptations improve the efficiency of carbon and iron utilization, thereby facilitating N2fixation and growth inTrichodesmium. This study sheds light on howTrichodesmiumand other N2-fixing microorganisms can optimize their physiological processes in response to environmental challenges.HIGHLIGHTSWe developed a metabolic flux model ofTrichodesmium, which resolves Dynamic cellular Permeability to O2(DPO2).DPO2increases N2fixation and growth rates.DPO2increases growth efficiency by reducing carbon wasting processes such as photorespiration and respiratory protection.DPO2, as a result, also increases iron utilization efficiency.
Uncorking the psychological factors behind habits and NoLo wine preferences
Authors: Rai Krupa , Galati Antonino , Chatterjee Sheshadri , Chaudhuri Ranjan , Vrontis Demetris , Migliore Giuseppina 
Published: February 2025

PurposeThe consumption of no-and low-alcohol (NoLo) wines has emerged as a growing trend among Italian millennials, reflecting a shift towards healthier and more mindful drinking habits. This paper delves into the social factors driving this trend, employing the rational of habit theory to provide a comprehensive understanding of NoLo wines consumption as a social behaviour among Italian millennials.Design/methodology/approachThe research design comprises symmetrical and asymmetrical approach. The study employs Cluster and fsQCA to understand the granularities of NoLo wine consumption.FindingsThe present study reveals that young adults are willingness to pay (WTP) more for NoLo wine in exchange of improved health benefits as a reward. The contrarian observation of relatively consumers with low average income exhibiting the high willingness to pay more for NoLo wine.Research limitations/implicationsThe sample is limited to Italy and skewed in terms of NoLo wine consumption. The participants were aware of NoLo wine but did not consume it in the past year. The study identified the paradoxical stance of low average family income group exhibiting more willingness-to-pay for de-alcoholised wine.Practical implicationsFirstly, young adults are increasingly health-conscious, seeking alternatives to alcohol that promote well-being and reduce health risks associated with excessive alcohol consumption. Secondly, mindfulness practices have gained popularity among young adults for altering self-perception, leading to a preference for beverages that align with mindful consumption and stress reduction. NoLo wine offers a suitable alternative, providing a similar sensory experience without the intoxicating effects of alcohol.Social implicationsHabit theory, pioneered by James (1890), posits that habits are formed through a three-stage process: cue, routine and reward. In the context of NoLo wine consumption, cues can include social gatherings, dining occasions or stress relief triggers. The routine involves the act of consuming NoLo wine, while the reward stems from the perceived benefits of this wine, such as reduced alcohol intake, improved health and social acceptance. The prevalence of NoLo wine consumption among Italian young adults can be attributed to a confluence of social factors.Originality/valueThe paper attempts to evaluate the contrarian case using fsQCA and the conceptual configuration of necessary conditions, viz. self-perception, social norms and health-consciousness.
Global patterns in workplace incivility research: a bibliometric analysis
Authors: Verma Madhu , Yadav Priyanka 
Published: February 2025

Purpose
The purpose of this study is to conduct a comprehensive bibliometric analysis of workplace incivility research spanning from 1999 to 2023, examining publication trends, intellectual structure, and global collaboration patterns to synthesize fragmented knowledge and identify future research directions.

Design/methodology/approach
This study used the Bibliometrix R-package to analyze 1,322 publications from Scopus and Web of Science databases. It used descriptive analysis and network analysis to examine the field’s evolution.

Findings
The analysis revealed significant growth in workplace incivility research, particularly after 2008. Key themes included customer incivility, emotional exhaustion and job satisfaction. The USA led in research output, with strong international collaborations. The intellectual structure comprised four main clusters, reflecting the field’s multidisciplinary nature.

Research limitations/implications
Limitations included potential bias toward English-language publications and reliance on citation metrics. This study highlighted opportunities for more diverse international collaborations and suggested focusing on long-term effects, evidence-based interventions and technology-mediated incivility in future research.

Originality/value
This study provides a comprehensive bibliometric analysis of workplace incivility research from two major databases (Scopus and Web of Science), offering a holistic view of the field’s development and identifying critical knowledge gaps to inform future research directions.
Moderating influence of cultural values on the relationship between governance and risk perception in large-scale infrastructure development projects
Authors: Oyuga Salome , Ochieng Edward Godfrey , Ngene Geoffrey 
Published: February 2025

PurposeThis paper investigates the moderating influence of cultural values on the relationship between governance and risk in large-scale infrastructure development projects. It integrates cultural psychology theory into interactive governance theory as a moderator of managerial perceptions of external debt as an effective rule-based risk management framework for these projects.Design/methodology/approachMixed method integrating quantitative analysis with qualitative insights based on a survey of managerial perceptions in large-scale renewable energy, road and rail projects in Kenya and linear regression was used to test the hypothesis.FindingsManagerial perceptions of country risk, project-specific external debt structure, carbon risk and cultural values significantly influenced their infrastructure risk perceptions. Demographic factors such as gender, years of experience, project tenure, board membership and socio-economic settings moderately influenced these risk perceptions. With 597 responses, the study expands on interactive governance theory by showing that cultural values and certain demographic attributes among managers moderate their view of external debt as an effective rule-based risk management framework for large infrastructure projects.Practical implicationsCultural values must be appraised when tailoring governance incentives to bolster managerial productivity and performance in mitigating risks in collaborative infrastructure projects.Originality/valueThis paper supports the hypothesis that cultural values moderate the interaction between governance and risk when the historical context incentivises managers to select defensive social learning techniques such as herding to avoid reputational performance risks in collaborative infrastructure projects.
Toward employee green creativity in the hotel industry: implications of green knowledge sharing, green employee empowerment and green values
Authors: Ansong Abraham , Andoh Raphael Papa Kweku , Ansong Linda Obeng , Hayford Cecilia , Owusu Nester Kumiwaa 
Published: February 2025

PurposeThe purpose of this study was to investigate the implications of green knowledge sharing, green empowerment and participation and green values toward hotel employees’ green creativity.Design/methodology/approachData was gathered from operational level employees of hospitality facilities Ghana. Through the partial least square structural equation modeling, bias-corrected and accelerated (BCa) confidence intervals obtained from 10,000 bootstrap subsamples were used to determine the significance of the hypothesized paths. This was preceded by examining the quality of the measurement and structural models.FindingsIt was established that green knowledge sharing is a positive predictor of green empowerment and participation. Green empowerment and participation also positively predicted employee green behavior and also partially mediated the link between green knowledge sharing and employee green behavior. Green values also positively moderated the connection green knowledge sharing has with employee green behavior.Practical implicationsTo enhance the green creativity of their employees so that the sustainability objectives are attained, general managers in hotels should institute strategies that promote green knowledge sharing among their employees, ensure the green empowerment and participation of their employees and inculcate green values in their employees.Originality/valueThis study expands the hotel greening literature by establishing the mediating and moderating processes for employee green creativity which is underrepresented in the literature. Besides, it extends the social learning theory in a green context.
Entrepreneurial universities: a decision-making approach
Authors: Maral Muammer 
Published: February 2025

PurposeEntrepreneurship of universities has a critical role due to its important effects. However, there is a significant gap in the literature regarding the methodology for evaluating university entrepreneurship. Evaluating university entrepreneurship provides valuable information to policymakers and governments. This research presents an evaluation methodology for assessing university entrepreneurship using a hybrid multi-criteria decision-making (MCDM) approach.Design/methodology/approachFor this purpose, entrepreneurship data of 50 universities from Türkiye were used. The evaluation framework consists of 4 main criteria and 23 sub-criteria. The importance level was determined by weighting the main criterion and sub-criteria with the subjective Best-Worst Method (BWM) based on expert opinions and the objective CRiteria Importance Through Intercriteria Correlation (CRITIC) method. The importance levels were determined by weighting the main and sub-criteria using the subjective BWM based on expert opinions and the objective CRITIC method. The final rankings were obtained by integrating the different ranking values obtained with the Borda function.FindingsFindings revealed that the most important main criterion in the entrepreneurship of universities is economic and social contribution activities, and the most important sub-criterion is international patents. The proposed method ranked all universities according to entrepreneurship. Sensitivity and validation analyses proved that the method is robust, stable and consistent.Practical implicationsThis study also provides a new perspective to policymakers and decision-makers in evaluating university entrepreneurship.Originality/valueThis study, which uses a hybrid approach of MCDM methods to evaluate the entrepreneurship of universities, can be adapted to different national and regional higher education systems, and this study provides guidance to researchers in this regard.
Insects as Natural Hosts, Vectors and Reservoirs of Botulinum Neurotoxin‐Producing Clostridia and Their Non‐Toxinogenic Counterparts: Preliminary Evidence
Authors: Douillard François P. , Lanzoni Olivia , Duplouy Anne , Lindström Miia 
Published: February 2025

ABSTRACTInsects play a significant role in the transmission and spread of bacterial pathogens that cause various diseases in humans and animals. The relationship among insects, bacterial pathogens and diseases is complex and depends on the specificity of the pathogens. Some clostridial species produce botulinum neurotoxin (BoNT), which is responsible for paralytic botulism. However, the ecology of these bacterial species and their non‐toxinogenic phylogenetic counterparts remains unclear. This study specifically explored in silico evidence of the interconnection between BoNT‐producing Clostridia and their non‐toxinogenic counterparts with insects. Based on literature meta‐analysis, the mining of 16S rRNA amplicon and metagenomic sequencing datasets and a pilot feeding experiment in the Glanville fritillary butterfly, Melitaea cinxia, we propose that BoNT‐producing Clostridia and their non‐toxinogenic phylogenetic counterparts are carried internally and/or externally in different insect orders. While previous case studies have indicated associations between Clostridia and insects, this work provides a more comprehensive view of their occurrence. It also highlights the need for further multidisciplinary investigations to characterise the natural ecology of BoNT‐producing Clostridia and their non‐toxinogenic counterparts in insects.
Muscling in and Making Space: ‘Demonstrable Claims’ and ‘Jurisdictional Clipping’ in the Reconfiguration of Professional Jurisdictions in the Surgical Care of Older People
Authors: Waring Justin J. , Martin Graham P. 
Published: February 2025

ABSTRACTThis paper examines the micro‐processes of jurisdictional change in the eco‐systems of healthcare work. This qualitative study investigated the expansion of geriatrician involvement in the perioperative pathway for older people. This study shows how, in response to opposition from surgeons and anaesthetists, geriatricians developed linked strategies that involved claiming the medical needs of surgical patients, and simultaneously integrating geriatric expertise into the non‐surgical peripheries of the pathway. By progressively demonstrating their ability to mitigate risks and improve surgical outcomes, geriatricians acquired an expanded role in the care pathway. This paper develops the concepts of ‘demonstrable claims’ and ‘jurisdictional clipping’ to explain the strategies of jurisdictional expansion. It also problematises these strategies by suggesting that role expansion was controlled and contained by more powerful incumbent groups, whereby the expansion of work was limited to temporal and spatial peripheries that were less valued by surgeons or anaesthetists.
Feasibility of a Standardised Mid‐Trimester Ultrasound Protocol: A National Multicenter Study
Authors: Bultez Thierry , Salomon Laurent Julien , Mahallati Houman , Fries Nicolas ,   the CNEOFT2 Study Group
Published: February 2025

ABSTRACTObjectiveEvaluate the feasibility and quality of a national standardised mid‐trimester ultrasound protocol using a consensus‐based quality assessment (QA) scoring system.DesignMulticenter prospective observational ‘FLASH’ study.Setting(i) Assessing the feasibility of a standardised protocol of 24 views at the mid‐trimester scan, with 21 recommended and 3 additional views, in routine practice.(ii) Assessing the quality of these images by evaluating the presence of conformity criteria.(iii) Analysing the reliability between self‐assessment and peer‐assessment of the images.PopulationA total of 440 mid‐trimester scans.MethodsA consensus‐based QA scoring system comprising 73 conformity criteria was established with 28 experts using a 3‐round Delphi method. Secondly, we asked operators to record 5 consecutive routine mid‐trimester scans. Images were analysed by the sonographer themselves and by a qualified expert according to the scoring system. The frequency of recorded images was calculated for each of the views. Factors associated with missing images per scan were evaluated. The robustness of conformity criteria was assessed by reliability between self‐evaluation and peer‐evaluation.Main outcome measuresBased on 9849 images, we observed feasibility of the 21 recommended standardised views for mid‐trimester scan ranging from 88.5% to 100%.ResultsMost conformity criteria (64/73, 88%) were met in over 90% of cases. Gwet's AC1 correlation between expert evaluation (peer‐evaluation) and participant evaluation (self‐evaluation) was greater than 0.80 for 70/73 (96%) criteria.ConclusionThis large‐scale 2‐month ‘flash’ observational study demonstrates the feasibility and quality of a national standardised mid‐trimester ultrasound protocol using a consensus‐based QA scoring system.
Influence of Sunlight and Shade on Decomposition and Insect Colonization
Authors: Park Woong‐Bae , Moon Tae‐Young , Park Sang‐Hyun , Do Yuno 
Published: February 2025

ABSTRACTThis study investigates the influence of microenvironmental factors on carcass decomposition and insect colonization, emphasizing the detailed analysis of insect communities. The research involved placing two pig carcasses in contrasting environments: one in a sunlit open field and the other in a shaded forest area. Over 10 days, we observed variations in decomposition rates, with the carcass in the sunlit area decomposing faster than the one in the shade. This research applies Symbolic Aggregate approXimation to transform the daily measurements of carcass weight into symbolic sequences, enabling a comparison of temporal patterns in insect colonization and decomposition dynamics between the environments. Additionally, we utilized alpha and beta diversity assessments to explore the composition and behavior of the insect populations. Our findings revealed dynamic shifts in insect diversity in the sunlit area, marked by a rapid increase followed by a decline, contrasting with steadier, gradual changes in the shaded area. These shifts underscore the sensitivity of insect communities to subtle differences in sunlight and temperature exposure. Our study confirms the significant impact of these environmental factors on both decomposition rates and insect community dynamics, highlighting their potential to refine post‐mortem interval estimations in forensic entomology. This enhances our understanding of how environmental conditions directly influence insect colonization, offering valuable insights for forensic applications.
Functional yacon juice fermented by Lactiplantibacillus plantarum QS7T: Chemical composition, flavor volatiles, and gut microbiota modulation
Authors: Zhu Yuan‐ting , Ma Qian , Zhang Xin‐yu , Gong Gui‐zhen , Wang Yi‐xin , Zeng Lin‐zhi , Li Rui‐tong , Xiao Chen , Zuo Yong 
Published: February 2025

AbstractTo prepare functional yacon juice, this study investigates the optimal fermentation duration, shelf‐life, chemical composition, and flavor volatiles of yacon juice fermented by Lactiplantibacillus plantarum QS7T capable of utilizing fructo‐oligosaccharides (FOS), along with its effects on gut microbiota. The optimal fermentation period was determined as 30 h, resulting in essential parameters: 3.46 g L−1 for total acids and a viable lactic acid bacteria (LAB) count of 2.3 × 108 CFU mL−1. The predicted shelf life when stored at 4°C is 28 days. Throughout storage, the juice maintained significant FOS and viable LAB count (8.0 × 107 CFU mL−1). Total short‐chain fatty acid significantly increased post‐fermentation and storage, reaching 177.86 µg mL−1. The total content and types of volatile components in yacon juice after fermentation by L. plantarum QS7T significantly increased, thus altering the flavor and improving the juice's appeal. Functional yacon juice also induced significant alterations in gut microbiota structure and abundance in mice, including increasing the abundance of beneficial microbes such as Firmicutes, Actinobacteriota, Faecalibaculum, Dubosiella, Bifidobacterium, and Akkermansia. These results indicate fermented yacon juice's potential as a functional food for gut microbiota modulation and anti‐inflammatory benefits.