bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of plant nutrient stoichiometry to fertilization varied with plant tissues in a tropical forest
Authors: Mo Qifeng , Zou Bi , Li Yingwen , Chen Yao , Zhang Weixin , Mao Rong , Ding Yongzhen , Wang Jun , Lu Xiankai , Li Xiaobo , Tang Jianwu , Li Zhian , Wang Faming 
Published: September 2015

AbstractPlant N:P ratios are widely used as indices of nutrient limitation in terrestrial ecosystems, but the response of these metrics in different plant tissues to altered N and P availability and their interactions remains largely unclear. We evaluated changes in N and P concentrations, N:P ratios of new leaves (<1 yr), older leaves (>1 yr), stems and mixed fine roots of seven species after 3-years of an N and P addition experiment in a tropical forest. Nitrogen addition only increased fine root N concentrations. P addition increased P concentrations among all tissues. The N × P interaction reduced leaf and stem P concentrations, suggesting a negative effect of N addition on P concentrations under P addition. The reliability of using nutrient ratios as indices of soil nutrient availability varied with tissues: the stoichiometric metrics of stems and older leaves were more responsive indicators of changed soil nutrient availability than those of new leaves and fine roots. However, leaf N:P ratios can be a useful indicator of inter-specific variation in plant response to nutrients availability. This study suggests that older leaf is a better choice than other tissues in the assessment of soil nutrient status and predicting plant response to altered nutrients using nutrients ratios.
The role of shear in crystallization kinetics: From suppression to enhancement
Authors: Richard David , Speck Thomas 
Published: September 2015

AbstractIn many technical applications crystallization proceeds in the presence of stresses and flows, hence the importance to understand the crystallization mechanism in simple situations. We employ molecular dynamics simulations to study the crystallization kinetics of a nearly hard sphere liquid that is weakly sheared. We demonstrate that shear flow both enhances and suppresses the crystallization kinetics of hard spheres. The effect of shear depends on the quiescent mechanism: suppression in the activated regime and enhancement in the diffusion-limited regime for small strain rates. At higher strain rates crystallization again becomes an activated process even at densities close to the glass transition.
Development of ‘Redox Arrays’ for identifying novel glutathionylated proteins in the secretome
Authors: Mullen Lisa , Seavill Miles , Hammouz Raneem , Bottazzi Barbara , Chan Philippe , Vaudry David , Ghezzi Pietro 
Published: September 2015

AbstractProteomics techniques for analysing the redox status of individual proteins in complex mixtures tend to identify the same proteins due to their high abundance. We describe here an array-based technique to identify proteins undergoing glutathionylation and apply it to the secretome and the proteome of human monocytic cells. The method is based on incorporation of biotinylated glutathione (GSH) into proteins, which can then be identified following binding to a 1000-protein antibody array. We thus identify 38 secreted and 55 intracellular glutathionylated proteins, most of which are novel candidates for glutathionylation. Two of the proteins identified in these experiments, IL-1 sRII and Lyn, were then confirmed to be susceptible to glutathionylation. Comparison of the redox array with conventional proteomic methods confirmed that the redox array is much more sensitive and can be performed using more than 100-fold less protein than is required for methods based on mass spectrometry. The identification of novel targets of glutathionylation, particularly in the secretome where the protein concentration is much lower, shows that redox arrays can overcome some of the limitations of established redox proteomics techniques.
Implementation of an antenatal magnesium sulfate protocol for fetal neuroprotection in preterm infants
Authors: Bouet Pierre-Emmanuel , Brun Stéphanie , Madar Hugo , Baisson Anne-Laure , Courtay Véronique , Gascoin-Lachambre Géraldine , Lasocki Sigismond , Sentilhes Loïc 
Published: September 2015

AbstractThe aim of our study was to assess the feasibility of implementing a protocol for the use of magnesium sulfate to prevent cerebral palsy. This retrospective single-center study included all women with fetuses of gestational age <33 weeks of gestation whose birth was planned or expected within 24 hours from September 2011 to December 2012. They were to receive magnesium sulfate, administered intravenously as a 4-g bolus followed by a constant infusion of 1 g per hour. If delivery had not occurred after 12 hours and was no longer considered imminent, the infusion was to be discontinued. The study included 119 women, 81 (68.1%) of whom received magnesium sulfate. Among the latter, 71 (87.5%) gave birth within 24 hours. The reasons treatment was not given were: omission by medical team (19/38, 50%), urgent delivery (18/38, 47.4%) and contraindication to treatment (1/38, 2.6%). The mean gestational age at protocol implementation was 29.6 +/− 2.1 weeks. Maternal monitoring, especially at the onset of infusion, appeared suboptimal. No major maternal side effects were observed. Our study shows that implementing a protocol for prevention of cerebral palsy by magnesium sulfate is feasible in a tertiary obstetric center.
Pathophysiology of major depressive disorder: mechanisms involved in etiology are not associated with clinical progression
Authors: Verduijn J , Milaneschi Y , Schoevers R A , van Hemert A M , Beekman A T F , Penninx B W J H 
Published: September 2015

AbstractMeta-analyses support the involvement of different pathophysiological mechanisms (inflammation, hypothalamic–pituitary (HPA)-axis, neurotrophic growth and vitamin D) in major depressive disorder (MDD). However, it remains unknown whether dysregulations in these mechanisms are more pronounced when MDD progresses toward multiple episodes and/or chronicity. We hypothesized that four central pathophysiological mechanisms of MDD are not only involved in etiology, but also associated with clinical disease progression. Therefore, we expected to find increasingly more dysregulation across consecutive stages of MDD progression. The sample from the Netherlands Study of Depression and Anxiety (18–65 years) consisted of 230 controls and 2333 participants assigned to a clinical staging model categorizing MDD in eight stages (0, 1A, 1B, 2, 3A, 3B, 3C and 4), from familial risk at MDD (stage 0) to chronic MDD (stage 4). Analyses of covariance examined whether pathophysiological mechanism markers (interleukin (IL)-6, C-reactive protein (CRP), cortisol, brain-derived neurotrophic factor and vitamin D) showed a linear trend across controls, those at risk for MDD (stages 0, 1A and 1B), and those with full-threshold MDD (stages 2, 3A, 3B, 3C and 4). Subsequently, pathophysiological differences across separate stages within those at risk and with full-threshold MDD were examined. A linear increase of inflammatory markers (CRP P=0.026; IL-6 P=0.090), cortisol (P=0.025) and decrease of vitamin D (P<0.001) was found across the entire sample (for example, from controls to those at risk and those with full-threshold MDD). Significant trends of dysregulations across stages were present in analyses focusing on at-risk individuals (IL-6 P=0.050; cortisol P=0.008; vitamin D P<0.001); however, no linear trends were found in dysregulations for any of the mechanisms across more progressive stages of full-threshold MDD. Our results support that the examined pathophysiological mechanisms are involved in MDD’s etiology. These same mechanisms, however, are less important in clinical progression from first to later MDD episodes and toward chronicity.
Effects of depressive symptoms and peripheral DAT methylation on neural reactivity to alcohol cues in alcoholism
Authors: Wiers C E , Shumay E , Volkow N D , Frieling H , Kotsiari A , Lindenmeyer J , Walter H , Bermpohl F 
Published: September 2015

AbstractIn alcohol-dependent (AD) patients, alcohol cues induce strong activations in brain areas associated with alcohol craving and relapse, such as the nucleus accumbens (NAc) and amygdala. However, little is known about the influence of depressive symptoms, which are common in AD patients, on the brain’s reactivity to alcohol cues. The methylation state of the dopamine transporter gene (DAT) has been associated with alcohol dependence, craving and depression, but its influence on neural alcohol cue reactivity has not been tested. Here, we compared brain reactivity to alcohol cues in 38 AD patients and 17 healthy controls (HCs) using functional magnetic resonance imaging and assessed the influence of depressive symptoms and peripheral DAT methylation in these responses. We show that alcoholics with low Beck’s Depression Inventory scores (n=29) had higher cue-induced reactivity in NAc and amygdala than those with mild/moderate depression scores (n=9), though subjective perception of craving was higher in those with mild/moderate depression scores. We corroborated a higher DAT methylation in AD patients than HCs, and showed higher DAT methylation in AD patients with mild/moderate than low depression scores. Within the AD cohort, higher methylation predicted craving and, at trend level (P=0.095), relapse 1 year after abstinence. Finally, we show that amygdala cue reactivity correlated with craving and DAT methylation only in AD patients with low depression scores. These findings suggest that depressive symptoms and DAT methylation are associated with alcohol craving and associated brain processes in alcohol dependence, which may have important consequences for treatment. Moreover, peripheral DAT methylation may be a clinically relevant biomarker in AD patients.
Cytotoxic effects of aspartame on human cervical carcinoma cells
Authors: Pandurangan Muthuraman , Enkhtaivan Gansukh , Kim Doo Hwan 
Published: September 2015

Abstract
Aspartame is used as an artificial sweetener in more than 6000 food varieties. The present study aims to determine the effects of aspartame at various concentrations on the cell viability, morphology, ROS level and DNA of human cervical carcinoma cells over two time periods of exposure. The effects of aspartame on HeLa cell viability were investigated using the sulphorhodamine-B assay (SRB assay) and flow cytometry. Alkaline comet assay was carried out to determine the possible DNA damage induced by aspartame. Mitochondria-derived reactive oxygen species (ROS) were determined using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). Fluorescence microscopy was used to determine the presence of apoptotic and necrotic cells following aspartame treatment. Cell viability was significantly altered following a higher concentration of aspartame exposure. Mitochondria-derived ROS increased at higher concentrations of aspartame exposure. Exposure to 10 mM and 20 mM of aspartame induced DNA fragmentation. Apoptotic and necrotic bodies were found in the range of 1–20 mM aspartame exposure. Exposure to high concentrations of aspartame may alter cell viability and morphology, and it may induce ROS generation and DNA damage in cervical carcinoma cells.
Plic-1, a new target in repressing epileptic seizure by regulation of GABAAR function in patients and a rat model of epilepsy
Authors: Zhang Yujiao , Li Zengyou , Gu Juan , Zhang Yanke , Wang Wei , Shen Hui , Chen Guojun , Wang Xuefeng 
Published: September 2015

Dysfunction of γ-aminobutyric acid A (GABAA) receptors (GABAARs) is a prominent factor affecting intractable epilepsy. Plic-1, an ubiquitin-like protein enriched in the inhibitory synapses connecting GABAARs and the ubiquitin protease system (UPS), plays a key role in the modification of GABAAR functions. However, the relationship between Plic-1 and epileptogenesis is not known. In the present study, we aimed to investigate Plic-1 levels in patients with temporal lobe epilepsy, as well as the role of Plic-1 in regulating onset and progression of epilepsy in animal models. We found that Plic-1 expression was significantly decreased in patients with epilepsy as well as pilocarpine- and pentylenetetrazol (PTZ)-induced rat epileptic models. Intrahippocampal injection of the PePα peptide, which disrupts Plic-1 binding to GABAARs, significantly shortened the latency of seizure onset, and increased the seizure severity and duration in these two epileptic models. Overexpressed Plic-1 through lentivirus transfection into a PTZ model resulted in a reduction in both seizure severity and generalized tonic–clonic seizure duration. Whole-cell clamp recordings revealed that the PePα peptide decreased miniature inhibitory postsynaptic currents (mIPSCs) whereas overexpressed Plic-1 increased mIPSCs in the pyramidal neurons of the hippocampus. These effects can be blocked by picrotoxin, a GABAAR inhibitor. Our results indicate that Plic-1 plays an important role in managing epileptic seizures by enhancing seizure inhibition through regulation of GABAARs at synaptic sites.
The Thoracolumbar AOSpine Injury Score
Authors: Kepler Christopher K. , Vaccaro Alexander R. , Schroeder Gregory D. , Koerner John D. , Vialle Luiz R. , Aarabi Bizhan , Rajasekaran Shanmuganathan , Bellabarba Carlo , Chapman Jens R. , Kandziora Frank , Schnake Klaus J. , Dvorak Marcel F. , Reinhold Max , Oner F. Cumhur 
Published: September 2015

Study Design Survey of 100 worldwide spine surgeons. Objective To develop a spine injury score for the AOSpine Thoracolumbar Spine Injury Classification System. Methods Each respondent was asked to numerically grade the severity of each variable of the AOSpine Thoracolumbar Spine Injury Classification System. Using the results, as well as limited input from the AOSpine Trauma Knowledge Forum, the Thoracolumbar AOSpine Injury Score was developed. Results Beginning with 1 point for A1, groups A, B, and C were consecutively awarded an additional point (A1, 1 point; A2, 2 points; A3, 3 points); however, because of a significant increase in the severity between A3 and A4 and because the severity of A4 and B1 was similar, both A4 and B1 were awarded 5 points. An uneven stepwise increase in severity moving from N0 to N4, with a substantial increase in severity between N2 (nerve root injury with radicular symptoms) and N3 (incomplete spinal cord injury) injuries, was identified. Hence, each grade of neurologic injury was progressively given an additional point starting with 0 points for N0, and the substantial difference in severity between N2 and N3 injuries was recognized by elevating N3 to 4 points. Finally, 1 point was awarded to the M1 modifier (indeterminate posterolateral ligamentous complex injury). Conclusion The Thoracolumbar AOSpine Injury Score is an easy-to-use, data-driven metric that will allow for the development of a surgical algorithm to accompany the AOSpine Thoracolumbar Spine Injury Classification System.
Solitary C1 Posterior Fixation for Unstable Isolated Atlas Fractures: Case Report and Systematic Review of the Literature
Authors: Bednar Drew A. , Almansoori Khaled A. 
Published: September 2015

Study Design A systematic review of the literature. Objectives To review the published results to date of motion-preserving direct reconstruction of C1 ring fractures with combined coronal plane displacement of at least 7 mm (rule of Spence) and so at risk for Dickman type I or II disruption of the transverse atlantal ligament (TAL). Methods A structured literature review prompted by successful management of a typical case. Results To date only 65 such cases are reported and follow-up is almost uniformly short. Although reported clinical success is uniform, the case mix is heterogenous and confirmation/classification of ligamentous injury at baseline is often lacking. Conclusions Direct C1 stabilization shows promise as a “more selective” option in managing displaced atlas fractures with probable TAL disruption but cannot yet be recommended as a practice standard. Prospective clinical studies are indicated and should be structured so as to differentiate between Dickman type I and type II injuries of the TAL.
Naturalization fosters the long-term political integration of immigrants
Authors: Hainmueller Jens , Hangartner Dominik , Pietrantuono Giuseppe 
Published: September 2015

SignificanceThe political integration of immigrant minorities is one of the most pressing policy issues many countries face today. Despite heated debates, there exists little rigorous evidence about whether naturalization fosters or dampens the integration of immigrants into the political fabric of the host society. Our study provides new causal evidence on the long-term effects of naturalization on political integration. Our research design takes advantage of a natural experiment in Switzerland that allows us to separate the independent effect of naturalization from the nonrandom selection into naturalization. We find that in our sample, naturalization caused long-lasting improvements in political integration, with immigrants becoming likely to vote and attaining considerably higher levels of political efficacy and political knowledge.
A parasitic nematode releases cytokinin that controls cell division and orchestrates feeding site formation in host plants
Authors: Siddique Shahid , Radakovic Zoran S. , De La Torre Carola M. , Chronis Demosthenis , Novák Ondřej , Ramireddy Eswarayya , Holbein Julia , Matera Christiane , Hütten Marion , Gutbrod Philipp , Anjam Muhammad Shahzad , Rozanska Elzbieta , Habash Samer , Elashry Abdelnaser , Sobczak Miroslaw , Kakimoto Tatsuo , Strnad Miroslav , Schmülling Thomas , Mitchum Melissa G. , Grundler Florian M. W. 
Published: September 2015

Significance
Sedentary plant-parasitic cyst nematodes are microscopic roundworms that cause significant yield losses in agriculture. Successful parasitism is based on the formation of a hypermetabolic feeding site in host roots from which the nematodes withdraw their nutrients. The host cell cycle is activated at the site of infection and contributes to the formation of the syncytium. Here, we provide genetic evidence that nematode-derived cytokinin is involved in activating the host cell cycle during infection. Our findings show the ability of an animal to synthesize and secrete a functional plant hormone to establish long-term parasitism.
Intermittent collective dynamics emerge from conflicting imperatives in sheep herds
Authors: Ginelli Francesco , Peruani Fernando , Pillot Marie-Helène , Chaté Hugues , Theraulaz Guy , Bon Richard 
Published: September 2015

SignificanceWe report and analyze quantitative field observations of large groups of Merino sheep. While grazing, these sheep must balance two competing needs: (i) the maximization of individual foraging space and (ii) the protection from predators offered by a large dense group. We show that they resolve this conflict by alternating slow foraging phases—during which the group spreads out—with fast packing events triggered by an individual-level behavioral shift. This leads to an intermittent collective dynamics with large density oscillations triggered by packing events on all accessible scales: a quasi-critical state. All our findings are well accounted for by an explicit model with individual behavioral shifts and strong allelomimetic properties.
Endothelial miR-17∼92 cluster negatively regulates arteriogenesis via miRNA-19 repression of WNT signaling
Authors: Landskroner-Eiger Shira , Qiu Cong , Perrotta Paola , Siragusa Mauro , Lee Monica Y. , Ulrich Victoria , Luciano Amelia K. , Zhuang Zhen W. , Corti Federico , Simons Michael , Montgomery Rusty L. , Wu Dianqing , Yu Jun , Sessa William C. 
Published: September 2015

Significance
MicroRNAs can repress target genes to regulate cellular function in vivo. The miR-17∼92 cluster is critical for cell cycle control, survival signaling, and angiogenesis, but the individual components of the cluster and their endogenous roles in vivo are less well understood. Here we show that the loss of endothelial-derived miR-17∼92 increases basal cardiac and limb arteriogenesis and improves ischemia-induced changes in blood flow. One component of the cluster, miR-19, directly targets FZD4 and LRP6, thereby regulating WNT signaling in vitro and in vivo, and antagonism of miR-19 up-regulates WNT signaling and improves defective blood flow recovery.
Global biogeography of human infectious diseases
Authors: Murray Kris A. , Preston Nicholas , Allen Toph , Zambrana-Torrelio Carlos , Hosseini Parviez R. , Daszak Peter 
Published: September 2015

SignificanceUnderstanding the distributions of infectious diseases is a central public and global health objective. We show that human infectious diseases exhibit striking biogeographic grouping patterns at a global scale, reminiscent of “Wallacean” zoogeographic patterns. This result is surprising, given the global distribution and unprecedented connectivity of humans as hosts and the homogenizing forces of globalization; despite these factors, infectious disease assemblages remain fundamentally constrained in their distributions by ecological barriers to dispersal or establishment. Biogeographic processes thus appear to provide an overarching context in which other factors promoting infectious disease emergence and spread are set. We use outbreaks of Ebola virus to illustrate how such patterns could be leveraged to provide a “head start” or added focus for risk management activities.
Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness
Authors: Ribeiro Alexandre J. S. , Ang Yen-Sin , Fu Ji-Dong , Rivas Renee N. , Mohamed Tamer M. A. , Higgs Gadryn C. , Srivastava Deepak , Pruitt Beth L. 
Published: September 2015

Significance
Human cardiomyocytes differentiated from pluripotent stem cells (hPSC-CMs) have potential as in vitro models of cardiac health and disease but differ from mature cardiomyocytes. In single live engineered hPSC-CMs with physiological shapes, we assayed the mechanical output and activity of sarcomeres and myofibrils in a nondestructive, noninvasive manner. Substrates with physiological stiffness improved contractile activity of patterned hPSC-CMs, as well as calcium flow, mitochondrial organization, electrophysiology, and transverse-tubule formation. The mechanical output and activity of sarcomeres and myofibrils varied as a function of mechanical cues and disrupted cell tension. This study establishes a high-throughput platform for modeling single-cell cardiac contractile activity and yields insight into environmental factors that drive maturation and sarcomere function in hPSC-CMs.
Glacial reduction and millennial-scale variations in Drake Passage throughflow
Authors: Lamy Frank , Arz Helge W. , Kilian Rolf , Lange Carina B. , Lembke-Jene Lester , Wengler Marc , Kaiser Jérôme , Baeza-Urrea Oscar , Hall Ian R. , Harada Naomi , Tiedemann Ralf 
Published: September 2015

Significance
The Drake Passage (DP) represents the most important oceanic gateway along the pathway of the world’s largest current: the Antarctic Circumpolar Current (ACC). Resolving changes in the flow of circumpolar water masses through the DP is crucial for advancing our understanding of the Southern Ocean’s role in affecting ocean and climate change on a global scale. We reconstruct current intensity from marine sediment records around the southern tip of South America with unprecedented millennial-scale resolution covering the past ∼65,000 y. For the last glacial period, we infer intervals of strong weakening of the ACC entering the DP, implying an enhanced export of northern ACC surface and intermediate waters into the South Pacific Gyre and reduced Pacific–Atlantic exchange through the cold water route.