bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Third French Alzheimer Plan: analysis of the influence of a national public health initiative on scientific research productivity and impact
Authors: Haeffner-Cavaillon Nicole , Devos Patrick , Ledoux Sylvie , Ménard Joël 
Published: September 2015

Abstract
Introduction
The Third National Alzheimer Plan (2008–2012) was a major public health initiative in France that included €200 million of funding for research in Alzheimer disease and related disorders (AD). The aim of this study was to document trends in French academic output in AD following the implementation of the plan.

Methods
Academic output (i.e., number of original articles) and scientific impact (i.e., article citations) of French research in AD were obtained from the Web of Knowledge core collection database. Analyses compared the 5-year period immediately before (2004–2008) and after (2009–2013) initiation of the plan. Comparisons were made with stroke, Parkinson disease, AIDS, and diabetes in the 14 leading countries worldwide and regionally within France.

Results
Worldwide production of original scientific articles between the periods 2004–2008 and 2009–2013 increased by 39 %, and that for AD increased by 46 %. China showed the largest increase and Japan the smallest. The absolute increase in French output on AD (54.6 %) was larger than that for stroke, Parkinson disease, AIDS, or diabetes. Globally, France had the third largest relative increase in output in AD (1.7-fold), behind only India (2.5-fold) and China (1.9-fold). There was a relative 2.3-fold increase in the proportion of French AD articles in the top 1 % globally most cited, larger than that for French articles on stroke, Parkinson disease, AIDS, or diabetes. At the national level, university hospitals participated in nearly 50 % of French AD publications. Analyses by geographical area demonstrated marked heterogeneity. We observed a strong correlation between level of funding and volume of output (R2 = 0.70), but not between funding and article impact (proportion of top 10 % globally cited articles; correlation R2 = 0.03).

Conclusions
Our study provides evidence of a specific positive impact of the funding provided by the Third National Alzheimer Plan in nearly doubling the global academic scientific output and increasing by 2.3-fold the top 1 % globally cited articles of France in AD research. Our bibliometric analyses provide objective and transparent information for policy makers on the relationship between research funding and academic output.
Verbal and non-verbal memory and hippocampal volumes in a memory clinic population
Authors: Bonner-Jackson Aaron , Mahmoud Shamseldeen , Miller Justin , Banks Sarah J 
Published: September 2015

Abstract
Introduction
Better characterization of the relationship between episodic memory and hippocampal volumes is crucial in early detection of neurodegenerative disease. We examined these relationships in a memory clinic population.

Methods
Participants (n = 226) underwent structural magnetic resonance imaging and tests of verbal (Hopkins Verbal Learning Test-Revised, HVLT-R) and non-verbal (Brief Visuospatial Memory Test-Revised, BVMT-R) memory. Correlational analyses were performed, and analyses on clinical subgroups (i.e., amnestic Mild Cognitive Impairment, non-amnestic Mild Cognitive Impairment, probable Alzheimer’s disease, intact memory) were conducted.

Results
Positive associations were identified between bilateral hippocampal volumes and both memory measures, and BVMT-R learning slope was more strongly positively associated with hippocampal volumes than HVLT-R learning slope. Amnestic Mild Cognitive Impairment (aMCI) participants showed specific positive associations between BVMT-R performance and hippocampal volumes bilaterally. Additionally, analyses of the aMCI group showed trend-level evidence of material-specific lateralization, such that retention of verbal information was positively associated with left hippocampal volume, whereas learning curve and retention of non-verbal information was positively associated with right hippocampal volume.

Conclusions
Findings support the link between episodic memory and hippocampal volumes in a memory clinic population. Non-verbal memory measures also may have higher diagnostic value, particularly in individuals at elevated risk for Alzheimer’s disease.
Sex differences in gait utilization and energy metabolism during terrestrial locomotion in two varieties of chicken (Gallus gallus domesticus) selected for different body size
Authors: Rose Kayleigh A. , Nudds Robert L. , Butler Patrick J. , Codd Jonathan R. 
Published: September 2015

ABSTRACTIn leghorn chickens (Gallus gallus domesticus) of standard breed (large) and bantam (small) varieties, artificial selection has led to females being permanently gravid and sexual selection has led to male-biased size dimorphism. Using respirometry, videography and morphological measurements, sex and variety differences in metabolic cost of locomotion, gait utilisation and maximum sustainable speed (Umax) were investigated during treadmill locomotion. Males were capable of greater Umax than females and used a grounded running gait at high speeds, which was only observed in a few bantam females and no standard breed females. Body mass accounted for variation in the incremental increase in metabolic power with speed between the varieties, but not the sexes. For the first time in an avian species, a greater mass-specific incremental cost of locomotion, and minimum measured cost of transport (CoTmin) were found in males than in females. Furthermore, in both varieties, the female CoTmin was lower than predicted from interspecific allometry. Even when compared at equivalent speeds (using Froude number), CoT decreased more rapidly in females than in males. These trends were common to both varieties despite a more upright limb in females than in males in the standard breed, and a lack of dimorphism in posture in the bantam variety. Females may possess compensatory adaptations for metabolic efficiency during gravidity (e.g. in muscle specialization/posture/kinematics). Furthermore, the elevated power at faster speeds in males may be linked to their muscle properties being suited to inter-male aggressive combat.
The lysosomal enzyme receptor protein (LERP) is not essential, but is implicated in lysosomal function inDrosophila melanogaster
Authors: Hasanagic Medina , van Meel Eline , Luan Shan , Aurora Rajeev , Kornfeld Stuart , Eissenberg Joel C. 
Published: September 2015

ABSTRACTThe lysosomal enzyme receptor protein (LERP) of Drosophila melanogaster is the ortholog of the mammalian cation-independent mannose 6-phosphate (Man 6-P) receptor, which mediates trafficking of newly synthesized lysosomal acid hydrolases to lysosomes. However, flies lack the enzymes necessary to make the Man 6-P mark, and the amino acids implicated in Man 6-P binding by the mammalian receptor are not conserved in LERP. Thus, the function of LERP in sorting of lysosomal enzymes to lysosomes in Drosophila is unclear. Here, we analyze the consequence of LERP depletion in S2 cells and intact flies. RNAi-mediated knockdown of LERP in S2 cells had little or no effect on the cellular content or secretion of several lysosomal hydrolases. We generated a novel Lerp null mutation, LerpF6, which abolishes LERP protein expression. Lerp mutants have normal viability and fertility and display no overt phenotypes other than reduced body weight. Lerp mutant flies exhibit a 30–40% decrease in the level of several lysosomal hydrolases, and are hypersensitive to dietary chloroquine and starvation, consistent with impaired lysosome function. Loss of LERP also enhances an eye phenotype associated with defective autophagy. Our findings implicate Lerp in lysosome function and autophagy.
The khmer software package: enabling efficient nucleotide sequence analysis
Authors: Crusoe Michael R. , Alameldin Hussien F. , Awad Sherine , Boucher Elmar , Caldwell Adam , Cartwright Reed , Charbonneau Amanda , Constantinides Bede , Edvenson Greg , Fay Scott , Fenton Jacob , Fenzl Thomas , Fish Jordan , Garcia-Gutierrez Leonor , Garland Phillip , Gluck Jonathan , González Iván , Guermond Sarah , Guo Jiarong , Gupta Aditi , Herr Joshua R. , Howe Adina , Hyer Alex , Härpfer Andreas , Irber Luiz , Kidd Rhys , Lin David , Lippi Justin , Mansour Tamer , McA'Nulty Pamela , McDonald Eric , Mizzi Jessica , Murray Kevin D. , Nahum Joshua R. , Nanlohy Kaben , Nederbragt Alexander Johan , Ortiz-Zuazaga Humberto , Ory Jeramia , Pell Jason , Pepe-Ranney Charles , Russ Zachary N. , Schwarz Erich , Scott Camille , Seaman Josiah , Sievert Scott , Simpson Jared , Skennerton Connor T. , Spencer James , Srinivasan Ramakrishnan , Standage Daniel , Stapleton James A. , Steinman Susan R. , Stein Joe , Taylor Benjamin , Trimble Will , Wiencko Heather L. , Wright Michael , Wyss Brian , Zhang Qingpeng , zyme en , Brown C. Titus 
Published: September 2015

The khmer package is a freely available software library for working efficiently with fixed length DNA words, or k-mers. khmer provides implementations of a probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization. khmer is implemented in C++ and Python, and is freely available under the BSD license at https://github.com/dib-lab/khmer/.
Primerjava poznavanja otrokovih pravic med starši hospitaliziranih otrok in medicinskimi sestrami
Authors: Kovač Sonja , Ferjan Marko 
Published: September 2015

Uvod: Pravice bolnih otrok imajo v modernih družbah status samostojnih pravic, ki jih je potrebno upoštevati in ustrezno varovati. Odvisne so od razvitosti družbe in zmogljivosti zdravstvenega sistema in bi jih morali poznati zdravstveni delavci, starši, skrbniki in otroci. Namen raziskave je bila primerjava poznavanja pravic otrok v bolnišnici med starši hospitaliziranih otrok in medicinskimi sestrami.
Metode: Uporabljena je bila neeksperimentalna metoda raziskovanja, podatki so bili zbrani z strukturiranim vprašalnikom na vzorcu staršev hospitaliziranih otrok (n = 49) in medicinskih sester (n = 60). Vprašalnik je bil predhodno testiran. Za analizo zbranih podatkov smo uporabili osnovno deskriptivno statistiko ter bivariatno analizo (test hi-kvadrat, t-test).
Rezultati: Raziskava pokaže statistično značilne razlike med starši in medicinskimi sestrami v stopnji seznanjenosti s pravicami bolnih otrok, ki temeljijo na Listini Evropskega združenja za pravice otrok v bolnišnicah (t = 5,828, p < 0,001).
Diskusija in zaključek: Poznavanje pravic otrok v bolnišnici je višje pri medicinskih sestrah kot pri starših hospitaliziranih otrok. Razlike so največje glede poznavanja načina komunikacije medicinskih sester s starši, glede razporeditve otrok na oddelke in ustvarjanja pogojev za igro ter izobraževanje v času bivanja v bolnišnici in glede pogojev sprejema v bolnišnico. Med obema skupinama ni razlik v oceni tega, v kolikšni meri se pravice otrok, ki temeljijo na Listini Evropskega združenja za pravice otrok v bolnišnicah, spoštujejo, obe skupini ocenjujeta, da je stopnja spoštovanja pravic dokaj visoka.
Mites and internal parasites associated with the common dung beetle Geotrupes (Anoplotrupes) stercorosus (Hartmann in Scriba, 1791) in Poland
Authors: Sulgostowska Teresa , Solarz Krzysztof , Madej Grażyna , Klimaszewski Krzysztof 
Published: September 2015

AbstractCommon dung beetles collected in the "Sobieski Forest" (eastern border of Warsaw suburbs) were examined for the occurrence and prevalence of infections or infestations with intestinal parasites and phoretic mites in relation to soil characteristics and quality of the forest habitat. Endoparasitic fauna was represented by gregarines Didymophyes paradoxa, microsporidians Plistophora geotrupina and cysticerkoids of 2 tapeworms - Ditestolepis diaphana and Staphylocystis furcate. Prevalence of these infections was higher for beetles collected from rich habitats. Acarofauna was represented by hypopodes of Sancassania geotruporum (Astigmatina, Acaridae) and the following taxa of mesostigmatic mites: Alliphis halleri, Macrocheles glaber, Parasitus coleoptratorum and unidentified juvenile Laelapidae representative. Mites were most abundant in June, July and September. They were only slightly more numerously found on dung beetles from the rich habitats. Nonmetric multidimensional scaling, MDS (2D stress = 0.13) revealed significant similarities in the distribution of mite taxa between poor and rich sites and among the investigated months (June, July and September).
Coexistence of Borrelia burgdorferi s.l. genospecies within Ixodes ricinus ticks from central and eastern Poland
Authors: Sytykiewicz Hubert , Karbowiak Grzegorz , Chorostowska-Wynimko Joanna , Szpechciński Adam , Supergan-Marwicz Marta , Horbowicz Marcin , Szwed Magdalena , Czerniewicz Paweł , Sprawka Iwona 
Published: September 2015

AbstractThe purpose of the study was to assess the prevalence and coinfection rates of Borrelia burgdorferi sensu lato genotypes in Ixodes ricinus (L.) ticks sampled from diverse localities in central and eastern regions of Poland. In years 2009-2011, questing nymphs and adults of I. ricinus were collected using a flagging method at 18 localities representing distinct ecosystem types: urban green areas, suburban forests and rural woodlands. Molecular detection of B. burgdorferi s.l. genospecies was based on amplification of a fla gene using nested PCR technique, subsequent PCR-RFLP analysis and bidirectional sequencing. It was revealed that 45 samples (2.1%) harboured two different B. burgdorferi s.l. genospecies, whereas triple infections with various spirochetes was found in 11 (0.5%) individuals. Generally, the highest average coinfection rates were evidenced in arachnids gathered at rural woodlands, intermediate at suburban forests, while the lowest were recorded at urban green areas. Overall, single spirochete infections were noted in 16.3% (n = 352/2,153) ticks. Importantly, it is the first report evidencing the occurrence of Borrelia miyamotoi (0.3%, n = 7/2153) in I. ricinus populations within central Poland. Circumstantial variability of B. burgdorferi s.l. genospecies in the common tick individuals sampled at various habitat types in central and eastern Poland was displayed. The coexistence of two or three different spirochete genospecies in single adult ticks, as well as the presence of B. miyamotoi were demonstrated. Therefore, further studies uncovering the co-circulation of the tested bacteria and other human pathogens in I. ricinus ticks are required.
Auto-infection by Echinostoma spp. cercariae in Helisoma anceps
Authors: Zimmermann Michael R. , Luth Kyle E. , Esch Gerald W. 
Published: September 2015

AbstractAuto-infection is a life history strategy used by many parasitic organisms, including digenetic trematodes. The process of autoinfection most frequently involves the transfer of a life cycle stage of the parasite from one site to another inside the same host, usually accompanied by morphological transformation. Moreover, among trematodes, the stage being transferred may also move from one host to another in completing the life cycle, i.e., an indirect cycle. Echinostoma spp. parasites offer the opportunity to study auto-infection because they utilize gastropods as both first and second intermediate hosts. Rejection of a null model predicting independent infection of first and second intermediate larval stages coupled with the presence of rediae being the best predictor of metacercariae prevalence and intensity suggests that auto-infection by Echinostoma spp. cercariae is occurring in their molluscan hosts. Shell length was also found to be a significant predictor of metacercariae intensity in the snails hosts, but this is most likely attributed to larger snails being more commonly infected with Echinostoma spp. rediae as opposed to an increased likelihood of cercariae infection. Auto-infection as a life history strategy increases transmission success of the parasite, but may also have negative consequences for the parasite that necessitate auto-infection coupled with the release of cercariae to maximize transmission success and host survival.
Cardiopulmonary helminths in foxes from the Pyrenees
Authors: Garrido-Castañé Ignasi , Ortuño Anna , Marco Ignasi , Castellà Joaquim 
Published: September 2015

AbstractThe present survey was carried out to investigate the prevalence of cardiopulmonary helminths in red foxes in Pyrenees area and to evaluate the role of foxes in the eco-epidemiology of these nematodes. Hearts and entire respiratory tracts were obtained from 87 foxes from Vall d’Aran region, Pyrenees, Catalonia, north-eastern Spain. The cardiopulmonary tracts were dissected, flushed and examined for nematodes using sedimented flushing water. Of the 87 examined foxes, 53 (61%) were positive for cardiopulmonary helminths. The identified nematodes were Crenosoma vulpis (44.8%), Eucoleus aerophilus (29.9%) and Angiostrongylus vasorum (3.4%). Statistical differences were observed only on comparing age and C.vulpis prevalence, with young foxes being more infected than adults. The high prevalence of cardiopulmonary nematodes suggested that red foxes may play an important role in their transmission and maintenance in the studied area.
Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids
Authors: Kumar Jaideep , Chaudhury Ashok , Bera Bidhan C. , Kumar Ritesh , Kumar Rajender , Tatu Utpal , Yadav Suresh Chandra 
Published: September 2015

AbstractThe present immuno-diagnostic method using soluble antigens from whole cell lysate antigen for trypanosomosis have certain inherent problems like lack of standardized and reproducible antigens, as well as ethical issues due to in vivo production, that could be alleviated by in vitro production. In the present study we have identified heat shock protein 70 (HSP70) from T. evansi proteome. The nucleotide sequence of T. evansi HSP70 was 2116 bp, which encodes 690 amino acid residues. The phylogenetic analysis of T. evansi HSP70 showed that T. evansi occurred within Trypanosoma clade and is most closely related to T. brucei brucei and T. brucei gambiense, whereas T. congolense HSP70 laid in separate clade. The two partial HSP70 sequences (HSP-1 from N-terminal region and HSP-2 from C-terminal region) were expressed and evaluated as diagnostic antigens using experimentally infected equine serum samples. Both recombinant proteins detected antibody in immunoblot using serum samples from experimental infected donkeys with T. evansi. Recombinant HSP-2 showed comparable antibody response to Whole cell lysate (WCL) antigen in immunoblot and ELISA. The initial results indicated that HSP70 has potential to detect the T. evansi infection and needs further validation on large set of equine serum samples.
Malondialdehyde; Lipid peroxidation plasma biomarker correlated with hepatic fibrosis in human Schistosoma mansoni infection
Authors: Aziz Inas Abdel , Yacoub Mariam , Rashid Laila , Solieman Ahmad 
Published: September 2015

AbstractSchistosomiasis is a debilitating parasitic disease, affects large number of host species. Currently affects 250-300 million people in tropic areas. Schistosoma pathogenic impact is hepatic periportal fibrosis; the parasite-induced inflammatory cellular activation promotes oxidative stress, resulting in lipid peroxidation (LPO), with subsequent increase in inflammatory mediators as malondialdehyde (MDA). This study was set up to reveal possible contribution of lipid peroxidation byproducts MDA in hepatic pathophysiology. Results displayed that MDA don’t tend to change in relation with either age, nor hepatic transaminases AST & ALT, while exhibited a significant increase in MDA levels in human schistosomiasis versus control group P<0.0001 (Mn. ± St.dev. 7.77 ± 3.59, 1.21 ± 0.28 nmol/ml) respectively. Moreover; MDA plasma levels in Schistosoma infected group correlated significantly with two hepatic fibrosis parameters; (a) ultrasonography graded periportal fibrosis P< 0.0001. Levels of MDA in hepatic fibrosis grades 0, I, II, III in Schistosoma infected group were (Mn. ± St.dev. 2.8 ± 0.64, 4.3 ± 1.2, 9.3 ± 1.6 and 10.8 ± 1.3 nmol/ml) respectively, (b) serum Hyaluronic acid (HA) P<0.0001 (spearman r = 0.77) as a reliable hepatic fibrosis marker. This implies a considerable role of LPO byproducts in schistosomiasis pathogenicity, and proposing malondialdehyde as a biomarker for schistosomiasis morbidity.
A new species of the feather mite genus Tyrannidectes Mironov, 2008 (Acariformes: Proctophyllodidae) from tapaculos (Passeriformes: Rhinocryptidae) in Chile
Authors: Mironov Sergey V. , González-Acuña Daniel A. 
Published: September 2015

AbstractA new feather mite species, Tyrannidectes pteroptochi sp. n. (Astigmata: Proctophyllodidae: Pterodectinae), is described from the Moustached Turca Pteroptochos megapodius Kittlitz, 1830 (Passeriformes: Rhinocryptidae) in Chile. This is the first record of mites of the genus Tyrannidectes Mironov 2008 from this family of passerines. Tyrannidectes pteroptochi is clearly distinguished from all previously known Tyrannidectes species by having several unique features in females: whip-like hysteronotal macrosetae h2, hysteronotal setae h3 exceeding the length of the terminal appendages, and spiculiform pseudanal setae ps2 and ps3. A brief review of taxonomic works on the Tyrannidectes is provided.
Preparation and Characterization of Low Temperature Heat-Treated 45S5 Bioactive Glass-Ceramic Analogues
Authors: Xie Kailuo , Zhang Lei , Yang Xianyan , Wang Xiaoqing , Yang Guojing , Zhang Licheng , Shao Huifeng , He Yong , Fu Jianzhong , Gou Zhongru 
Published: September 2015

AbstractThe 45S5 Bioglassr and its sintered bioactive glass-ceramic (BGC) have been widely investigated as bone implants, mainly for its ability to bond to hard tissues. However, high temperature treatment is not enough to improve its poor mechanical properties, but compromise its biologically relevant performances. The innovative BGC compositions based on the thermally treated 45S5 Bioglassr were developed by decreasing the P2O5 quantity and adding B2O3 (0-6%) into the Na2O–2CaO–3SiO2- based bioactive glasses (BG). The thermally treated BGCs were fully characterized from the microstructural and mechanical points of view and compared to each other. Their bioactivity and bio-dissolutionwere established by means of in vitro soaking tests. The new B2O3-added 45S5 BG analogues, named NCS-xB, can be transformed to crystalline phase (Na2Ca2Si3O9)-based BGCs of high compactness and bioactivity at a relatively low temperature heat treatment (≤ 900ºC), since their bioactivity is preserved. Our experimental results suggest that the new 45S5 BGC analogues with optimized composition exhibit improved micro- structural and mechanical properties, and are beneficial for making specific products such as porous scaffolds or composites for bone defect repair.