bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progressive alopecia associated with hypoparathyroidism in a Jersey heifer
Authors: Villarroel Aurora , Hustace Jaime L. , Piripi Susan A. 
Published: May 2013

BackgroundChronic progressive generalized alopecia in dairy cattle has been anecdotally discussed in veterinary forums.ObjectiveTo describe subclinical changes in an otherwise apparently healthy animal with chronic progressive generalized alopecia.AnimalsOne 10‐month‐old Jersey heifer.MethodsCase report.ResultsA heifer was presented for chronic progressive alopecia. The skin was hyperpigmented and very thin (2.4 ± 0.0 mm, compared with 8.1 ± 0.3 mm measured in four contemporaneous Jersey heifers). Histopathological examination of the skin revealed severe epidermal hyperplasia, orthokeratotic hyperkeratosis and diffuse follicular atrophy. Clinicopathological changes included hypocalcaemia that did not respond to calcium therapy. Serum parathyroid hormone (PTH) was not detected, compared with 1.38 ± 0.78 pmol/L in four contemporaneous Jersey heifers. A diagnosis of hypoparathyroidism was made based on hypocalcaemia and low PTH in the presence of normal magnesium levels. The heifer was otherwise healthy and was allowed to continue normal life. A spontaneous abortion occurred at 5.5 months of pregnancy and this was likely due to the presence of twin fetuses. Despite targeted therapy of the ensuing retained placenta, the heifer declined in health and had to be euthanized. From the time of diagnosis with hypoparathyroidism until euthanasia (29 months of age), blood levels of calcium, phosphorus and PTH were regularly monitored. Calcium and phosphorus levels varied widely. Serum PTH was consistently undetectable. At postmortem examination, the parathyroid glands could not be located.Conclusions and clinical importanceTo our knowledge, this is the first report of naturally occurring hypoparathyroidism in cattle and with a clinical presentation of alopecia.
Václav Šimerka: quadratic forms and factorization
Authors: Lemmermeyer F. 
Published: May 2013

AbstractIn this article we show that the Czech mathematician Václav Šimerka discovered the factorization of$\frac{1}{9} (1{0}^{17} - 1)$using a method based on the class group of binary quadratic forms more than 120 years before Shanks and Schnorr developed similar algorithms. Šimerka also gave the first examples of what later became known as Carmichael numbers.
Complete Genome Sequence of a Natural Recombinant H9N2 Influenza Virus Isolated from a White-Fronted Goose (
Anser albifrons
) in South Korea
Authors: Lee Dong-Hun , Park Jae-Keun , Yuk Seong-Su , Erdene-Ochir Tseren-Ochir , Kwon Jung-Hoon , Lee Joong-Bok , Park Seung-Yong , Choi In-Soo , Song Chang-Seon 
Published: May 2013

ABSTRACT

In 2007, we isolated a natural recombinant H9N2 avian influenza virus (AIV) from the fecal droppings of a white-fronted goose (
Anser albifrons
) in South Korea. Phylogenetic analyses of the complete genome sequence showed that polymerase acidic (PA) and neuraminidase (NA) genes belonged to the Eurasian lineage AIV, but polymerase basic 2 (PB2), PB1, hemagglutinin (HA), nucleoprotein (NP), matrix (M), and nonstructural (NS) genes belonged to the North-American lineage AIV. These data are beneficial for understanding the ecology and epidemiology of AIVs.
Complete Genomic Sequence of Southern Rice Blacked-Dwarf Virus, a Novel Fijivirus, from Vietnam
Authors: Xue Jin , Li Jing , Ta Hoang-Anh , Zhang Heng-Mu , Yang Jian , Lv Ming-Fang , Meng Yuan , Li Pei-Pei , Chen Jian-Ping 
Published: May 2013

ABSTRACT
The nucleotide sequences of the ten genomic segments of a Vietnam isolate of southern rice blacked-dwarf virus were determined. This complete genomic sequence will help to further understand the viral etiology (origin of viral pathogen) and phylogenetic relationships among fijiviruses.
Genome Sequence of Xanthomonas arboricola pv. Corylina, Isolated from Turkish Filbert in Colorado
Authors: Ibarra Caballero Jorge , Zerillo Marcelo M. , Snelling Jacob , Boucher Christina , Tisserat Ned 
Published: May 2013

ABSTRACT

Previously, we reported the isolation of a bacterium producing leaf spots in Turkish filbert. Here, we present the draft genome assembly of the bacterium identified as
Xanthomonas arboricola
pv. corylina. To our knowledge, this is the first published genome of this pathovar of
X. arboricola
.
Genome Sequence of Salmonella enterica Serotype Tennessee Strain CDC07-0191, Implicated in the 2006-2007 Multistate Food-Borne Outbreak Linked to Peanut Butter in the United States
Authors: Deng Xiangyu , Salazar Joelle K. , Frezet Stephanie , MacCannell Duncan , Ribot Efrain M. , Fields Patricia I. , Fricke W. Florian , Zhang Wei 
Published: May 2013

ABSTRACT

Salmonella enterica
serotype Tennessee strain CDC07-0191 was isolated from the 2006-2007 multistate food-borne outbreak linked to peanut butter in the United States. Here we report a high-quality draft assembly of the genome sequence of this strain, derived from a patient. This is the first reported high-quality draft genome sequence for
S. enterica
serotype Tennessee, which will enable in-depth studies of its transmission and virulence.
Complete Genome Sequence of Corynebacterium urealyticum Strain DSM 7111, Isolated from a 9-Year-Old Patient with Alkaline-Encrusted Cystitis
Authors: Guimarães Luis C. , Soares Siomar C. , Albersmeier Andreas , Blom Jochen , Jaenicke Sebastian , Azevedo Vasco , Soriano Francisco , Tauch Andreas , Trost Eva 
Published: May 2013

ABSTRACT

Corynebacterium urealyticum
is a common skin colonizer with potent urease activity. It is clinically recognized as an opportunistic pathogen causing urinary tract infections. The annotated genome sequence of strain DSM 7111, isolated from the urine of a young boy with an ectopic kidney, provides new insights into the pathomechanisms of this bacterium.
Draft Genome Sequence of the Cellulolytic, Mesophilic, Anaerobic Bacterium Clostridium termitidis Strain CT1112 (DSM 5398)
Authors: Lal Sadhana , Ramachandran Umesh , Zhang Xiangli , Munir Riffat , Sparling Richard , Levin David B. 
Published: May 2013

ABSTRACT

Here, we report the draft genome sequence of
Clostridium termitidis
strain CT1112 (DSM 5398), a mesophilic, cellulolytic bacterium that can utilize a variety of sugars, as well as pure cellulose, as a sole carbon source; it also synthesizes fermentation end products with potential industrial applications.
Genome Sequence of Pseudomonas aeruginosa PA45, a Highly Virulent Strain Isolated from a Patient with Bloodstream Infection
Authors: Segata Nicola , Ballarini Annalisa , Jousson Olivier 
Published: May 2013

ABSTRACT

Pseudomonas aeruginosa
is a ubiquitous opportunistic pathogen causing a broad range of infections in humans. We provide the draft genome sequence of the recently identified and highly virulent
P. aeruginosa
PA45 strain. Its 6.6-Mb genome contains 6,822 genes, including an unparalleled number of virulence genes, which might explain its aggressive phenotype.
Co-infusion of adipose tissue derived mesenchymal stem cell-differentiated insulin-making cells and haematopoietic cells with renal transplantation: a novel therapy for type 1 diabetes mellitus with end-stage renal disease
Authors: Dave Shruti D , Vanikar Aruna V , Trivedi Hargovind L 
Published: May 2013

Type 1 diabetes mellitus (T1DM) is a common cause of end-stage renal disease (ESRD). Various factors contribute to wide fluctuations in blood glucose levels and exogenous insulin requirement in such patients even after renal transplantation (RT). Simultaneous pancreas–kidney transplantation is one of the therapies for these patients. Stem cell (SC) therapy for T1DM and for minimisation of immunosuppression after RT has shown encouraging results. We report a 30-year-old-man with T1DM since 15 years and ESRD since 2 years, who underwent living donor RT and co-infusion of in vitro generated insulin-making cells differentiated from donor adipose tissue derived mesenchymal stem cells and bone marrow -derived haematopoietic SC into subcutaneous tissue, portal and thymic circulation under non-myeloablative conditioning. Over follow-up of 13 months he has stable graft function with serum creatinine, 1.2 mg/dl, zero rejection and glycosylated haemoglobin level of 6.1% on calcineurin-inhibitor based therapy.
The Housestaff Incentive Program: improving the timeliness and quality of discharge summaries by engaging residents in quality improvement
Authors: Bischoff Kara , Goel Aparna , Hollander Harry , Ranji Sumant R , Mourad Michelle 
Published: May 2013

BackgroundQuality improvement has become increasingly important in the practice of medicine; however, engaging residents in meaningful projects within the demanding training environment remains challenging.MethodsWe conducted a year-long quality improvement project involving internal medicine residents at an academic medical centre. Resident champions designed and implemented a discharge summary improvement bundle, which employed an educational curriculum, an electronic discharge summary template, regular data feedback and a financial incentive. The timeliness and quality of discharge summaries were measured before and after the intervention. Residents and faculty were surveyed about their perceptions of the project; primary care providers were surveyed about their satisfaction with hospital provider communication.ResultsWith implementation of the bundle, the average time from patient discharge to completion of the discharge summary fell from 3.5 to 0.61 days (p<0.001). The percentage of summaries completed on the day of discharge rose from 38% to 83% (p<0.001) and this improvement was sustained for 6 months following the end of the project. The percentage of summaries that included all recommended elements increased from 5% to 88% (p<0.001). Primary care providers reported a lower likelihood of discharge summaries being unavailable at the time of outpatient follow-up (38% to 4%, p<0.001). Residents reported that the systems changes, more than the financial incentive, accounted for their behaviour change.ConclusionsOur discharge summary improvement project provides an instructive example of how residents can lead clinically meaningful quality improvement projects.
Cervical spine clearance in the elderly: do elderly patients get a bad deal?
Authors: Edwards M A , Verwey J , Herbert S , Horne S , Smith J E 
Published: May 2013

ObjectiveTo investigate whether elderly patients, presenting following trauma and requiring immobilisation and imaging of the cervical spine, require increased use of CT and spend longer immobilised when compared to the younger population.MethodsA retrospective chart review was undertaken of 35 adults aged 18–65 years, and 32 adults over 65 years, requiring cervical spine imaging following trauma.Results1 of the 35 younger patients, and 16 of the 32 elderly patients, underwent CT. Elderly patients spent 1:05 h longer immobilised (p<0.005).ConclusionsHalf of the elderly patients underwent CT, and they were immobilised for significantly longer than younger patients. Measures should be adopted to facilitate early diagnosis and mitigate complications of prolonged immobilisation in elderly patients with neck trauma.
The relationship between hyperlactatemia and microcirculation in the thenar eminence as measured using near-infrared spectroscopy in patients with sepsis
Authors: Soga Taketomo , Sakatani Kaoru , Yagi Tsukasa , Kawamorita Tsuyoshi , Yoshino Atsuo 
Published: May 2013

ObjectiveNear-infrared spectroscopy (NIRS)-derived variables during a vascular occlusion test (VOT) have recently been used to evaluate peripheral circulatory insufficiency in patients with sepsis. We investigated the relationship between NIRS-derived variables and the lactate concentration during sepsis.MethodsThis was a prospective observational study. Forty-one patients with sepsis were divided into two groups according to their blood lactate concentration: a high lactate group (≥2.0 mmol/l; n=15) and a normal lactate group (<2.0 mmol/l; n=26). NIRS was used to evaluate the tissue oxygen index (TOI) at the thenar eminence before and after a VOT. VOT was performed by sphygmomanometer cuff inflation around upper arm for 3 min. The TOI value before VOT was defined as the TOI baseline, the TOI value immediately after VOT was defined as the minimum TOI and the TOI value after releasing the muscle from the VOT was defined as the TOI after VOT. The ΔTOI was defined as the difference between the TOI after VOT and minimum TOI.ResultsNIRS-derived variables had significant differences between the normal lactate group and the high lactate group with respect to the TOI baseline (median: 65% vs 55%, p=0.01), TOI after VOT (median: 65% vs 46%, p<0.001) and ΔTOI (median: 19% vs 6%, p<0.001). The lactate concentration was significantly correlated with the TOI after VOT (R=0.594, p<0.001), TOI baseline (R=0.513, p<0.001), ΔTOI (R=0.488, p=0.001) and minimum TOI (R=0.377, p=0.02).ConclusionsWe revealed a significant correlation between NIRS-derived variables and the lactate concentration during sepsis.
Accuracy of visual assessments of proliferation indices in gastroenteropancreatic neuroendocrine tumours
Authors: Young Helen T M , Carr Norman J , Green Bryan , Tilley Charles , Bhargava Vidhi , Pearce Neil 
Published: May 2013

AimsTo compare the accuracy of eyeball estimates of the Ki-67 proliferation index (PI) with formal counting of 2000 cells as recommend by the Royal College of Pathologists.MethodsSections from gastroenteropancreatic neuroendocrine tumours were immunostained for Ki-67. PI was calculated using three methods: (1) a manual tally count of 2000 cells from the area of highest nuclear labelling using a microscope eyepiece graticule; (2) eyeball estimates made by four pathologists within the same area of highest nuclear labelling; and (3) image analysis of microscope photographs taken from this area using the ImageJ ‘cell counter’ tool. ImageJ analysis was considered the gold standard for comparison.ResultsLevels of agreement between methods were evaluated using Bland–Altman plots. Agreement between the manual tally and ImageJ assessments was very high at low PIs. Agreement between eyeball assessments and ImageJ analysis varied between pathologists. Where data for low PIs alone were analysed, there was a moderate level of agreement between pathologists’ estimates and the gold standard, but when all data were included, agreement was poor.ConclusionsManual tally counts of 2000 cells exhibited similar levels of accuracy to the gold standard, especially at low PIs. Eyeball estimates were significantly less accurate than the gold standard. This suggests that tumour grades may be misclassified by eyeballing and that formal tally counting of positive cells produces more reliable results. Further studies are needed to identify accurate clinically appropriate ways of calculating.
T-cell clonality is detected in a high frequency among patients with incidental lymphocytosis by PCR assays forTCRgene rearrangements
Authors: Cao Fenglin , Wang Shuye , Zhao Hui , Zhou Jin , Yang Guisheng , Wang Chen 
Published: May 2013

AimsClonal expansion of lymphocytes may account for asymptomatic lymphocytosis. Unlike monoclonal B lymphocytosis, the detection of T-cell clones is difficult using immunophenotype-based assays. This study aimed to evaluate PCR-based clonality assays for the identification of T-cell clones among patients with unexplained lymphocytosis.MethodsWe incorporated the BIOMED-2 multiplex PCR into the investigation of peripheral lymphocytosis. Clonality analysis was performed with three reactions forTCRβand two reactions forTCRγgene rearrangements. The analysis was performed with blood specimens from a total of 150 adult patients who presented with incidental lymphocytosis.ResultsPCR assays were validated using confirmed T-cell malignancies and the detection sensitivity was determined at a level of 2%. Using theTCRβandTCRγcombination, 25 (16.6%) of 150 patients were found to have clonalTCRarrangement. Patients who harboured clonal T cells presented with a mild to moderate absolute lymphocytosis, with a median lymphocyte count of 4.5 × 109/l (range 3.7–9.8 × 109) and the absence of other haematological abnormalities. Immunophenotyping confirmed T-cell lymphocytosis with an increase in CD8 T cells in the majority of patients.ConclusionsThis study demonstrated the use of PCR assays for the effective detection of clonal T lymphocytosis. Our data indicate a high prevalence of silent T-cell clones among patients with peripheral lymphocytosis.
Understanding perceptions of genital herpes disclosure through analysis of an online video contest
Authors: Catallozzi Marina , Ebel Sophia C , Chávez Noé R , Shearer Lee S , Mindel Adrian , Rosenthal Susan L 
Published: May 2013

ObjectiveThe aims of this study were to examine pre-existing videos in order to explore the motivation for, possible approaches to, and timing and context of disclosure of genital herpes infection as described by the lay public.MethodsA thematic content analysis was performed on 63 videos submitted to an Australian online contest sponsored by the Australian Herpes Management Forum and Novartis Pharmaceuticals designed to promote disclosure of genital herpes.ResultsVideos either provided a motivation for disclosure of genital herpes or directed disclosure without an explicit rationale. Motivations included manageability of the disease or consistency with important values. Evaluation of strategies and logistics of disclosure revealed a variety of communication styles including direct and indirect. Disclosure settings included those that were private, semiprivate and public. Disclosure was portrayed in a variety of relationship types, and at different times within those relationships, with many videos demonstrating disclosure in connection with a romantic setting.ConclusionsIndividuals with genital herpes are expected to disclose to susceptible partners. This analysis suggests that understanding lay perspectives on herpes disclosure to a partner may help healthcare providers develop counselling messages that decrease anxiety and foster disclosure to prevent transmission.
Genome-Wide Association Study of Genetic Predictors of Overall Survival for Non–Small Cell Lung Cancer in Never Smokers
Authors: Wu Xifeng , Wang Liang , Ye Yuanqing , Aakre Jeremiah A. , Pu Xia , Chang Gee-Chen , Yang Pan-Chyr , Roth Jack A. , Marks Randolph S. , Lippman Scott M. , Chang Joe Y. , Lu Charles , Deschamps Claude , Su Wu-Chou , Wang Wen-Chang , Huang Ming-Shyan , Chang David W. , Li Yan , Pankratz V. Shane , Minna John D. , Hong Waun Ki , Hildebrandt Michelle A.T. , Hsiung Chao Agnes , Yang Ping 
Published: May 2013

Abstract
To identify the genetic factors that influence overall survival in never smokers who have non–small cell lung carcinoma (NSCLC), we conducted a consistency meta-analysis study using genome-wide association approaches for overall survival in 327 never smoker patients with NSCLC from The University of Texas MD Anderson Cancer Center (Houston, TX) and 293 cases from the Mayo Clinic (Rochester, MN). We then conducted a two-pronged validation of the top 25 variants that included additional validation in 1,256 patients with NSCLC from Taiwan and assessment of expression quantitative trait loci (eQTL) and differential expression of genes surrounding the top loci in 70 tumors and matched normal tissues. A total of 94 loci were significant for overall survival in both MD Anderson and Mayo studies in the consistency meta-analysis phase, with the top 25 variants reaching a P value of 10−6. Two variants of these 25 were also significant in the Taiwanese population: rs6901416 [HR, 1.44; 95% confidence interval (CI), 1.01–2.06] and rs10766739 (HR, 1.23; 95%CI, 1.00–1.51). These loci resulted in a reduction of median survival time of at least eight and five months in three populations, respectively. An additional six variants (rs4237904, rs7976914, rs4970833, rs954785, rs485411, and rs10906104) were validated through eQTL analysis that identified significant correlations with expression levels of six genes (LEMD3, TMBIM, ATXN7L2, SHE, ITIH2, and NUDT5, respectively) in normal lung tissue. These genes were also significantly differentially expressed between the tumor and normal lung tissue. These findings identify several novel, candidate prognostic markers for NSCLC in never smokers, with eQTL analysis suggesting a potential biologic mechanism for a subset of these observed associations. Cancer Res; 73(13); 4028–38. ©2013 AACR.
Membrane versus Soluble Isoforms of TNF-α Exert Opposing Effects on Tumor Growth and Survival of Tumor-Associated Myeloid Cells
Authors: Ardestani Shidrokh , Li Bin , Deskins Desirae L. , Wu Huiyun , Massion Pierre P. , Young Pampee P. 
Published: May 2013

AbstractTNF-α, produced by most malignant cells, orchestrates the interplay between malignant cells and myeloid cells, which have been linked to tumor growth and metastasis. Although TNF-α can exist as one of two isoforms, a 26-kDa membrane tethered form (mTNF-α) or a soluble 17-kDa cytokine (sTNF-α), the vast majority of published studies have only investigated the biologic effects of the soluble form. We show for the first time that membrane and soluble isoforms have diametrically opposing effects on both tumor growth and myeloid content. Mouse lung and melanoma tumor lines expressing mTNF-α generated small tumors devoid of monocytes versus respective control lines or lines expressing sTNF-α. The lack of myeloid cells was due to a direct effect of mTNF-α on myeloid survival via induction of cell necrosis by increasing reactive oxygen species. Human non–small cell lung carcinoma expressed varying levels of both soluble and membrane TNF-α, and gene expression patterns favoring mTNF-α were predictive of improved lung cancer survival. These data suggest that there are significant differences in the role of different TNF-α isoforms in tumor progression and the bioavailability of each isoform may distinctly regulate tumor progression. This insight is critical for effective intervention in cancer therapy with the available TNF-α inhibitors, which can block both TNF-α isoforms. Cancer Res; 73(13); 3938–50. ©2013 AACR.