bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does speculum lubricant affect liquid‐based Papanicolaou test adequacy?
Authors: Lin Stephanie N. , Taylor Jolyn , Alperstein Susan , Hoda Rana , Holcomb Kevin 
Published: November 2013

BACKGROUNDThere is a paucity of data on the effect of vaginal lubricants on specimen adequacy in the era of liquid‐based Papanicolaou (Pap) tests. Current manufacturer recommendations advise against the use of lubricants, especially those that contain carbomers or carbopol polymers. There is, however, no conclusive evidence to support this recommendation. Moreover, the data that does exist is conflicting.METHODSRetrospective review was performed of all liquid‐based Pap tests collected between January 2010 and March 2012 by the Gynecologic Oncology division at a single hospital. Specimen adequacy was compared for vaginal and cervical specimens that were collected with and without a lubricant. Results were also compared for specimens collected with lubricants that did or did not contain carbomers.RESULTSThere was no significant difference in specimen adequacy for cervical or vaginal Pap test specimens collected with a water‐soluble lubricant not containing carbomers, compared to those collected without a lubricant. There was, however, a significantly higher rate of insufficient specimens when a water‐soluble lubricant containing carbomers was used. This difference was most pronounced for vaginal Pap tests (26.9% versus 1.2%, OR = 30.3, 95% CI = 16.6‐55.1, P < .0001).CONCLUSIONSLubricants containing carbomers should be avoided when collecting liquid‐based Pap tests because they can significantly decrease specimen adequacy. Lubricants that do not contain these substances do not significantly affect specimen adequacy. If there is an unusual increase in unsatisfactory Pap tests, the cytology laboratory should notify clinicians and work with them to determine the cause. Cancer (Cancer Cytopathol) 2014;122:221–226. © 2013 American Cancer Society.
Highly Selective Fluorescence Detection of Hydrogen Sulfide by Using an Anthracene‐Functionalized Cyclam–CuII Complex
Authors: Santos‐Figueroa Luis E. , de la Torre Cristina , El Sayed Sameh , Sancenón Félix , Martínez‐Máñez Ramón , Costero Ana M. , Gil Salvador , Parra Margarita 
Published: November 2013

AbstractAn anthracene‐functionalised cyclam–copper(II) complex for the detection of HS– in aqueous environments has been prepared. This probe displays poor fluorescence but can selectively and sensitively detect HS– anions in water over other anions, biothiols and common oxidants such as H2O2 through remarkably enhanced emission. This turn‐on response in the presence of the HS– anion is ascribed to a demetallation reaction that inhibits emission quenching observed in the initial complex as a result of the presence of the paramagnetic Cu2+ centre. Moreover, real‐time fluorescence imaging measurements confirm that probe [Cu(1)]2+ can be easily used to detect intracellular HS– at micromolar concentrations with a remarkable enhancement in the FI/FI0 ratio.
Pediatric hospitalist preoperative evaluation of children with neuromuscular scoliosis
Authors: Rappaport David I. , Cerra Samantha , Hossain Jobayer , Sharif Iman , Pressel David M. 
Published: November 2013

WHAT'S NEWThis is the first study of a pediatric hospitalist preoperative clinic. Pediatric hospitalists frequently make significant recommendations for patients with neuromuscular scoliosis prior to spinal surgery, especially those with medical complexity. Certain clinical criteria are statistically significantly associated with a hospitalist making a preoperative recommendation.OBJECTIVETo assess (1) how frequently pediatric hospitalists make recommendations when evaluating preoperative neuromuscular scoliosis patients in anticipation of spinal fusion surgery and (2) evaluate if any clinical characteristics are associated with a higher likelihood of hospitalists doing so.METHODSWe performed a case series study using retrospective chart review of 214 patients scheduled for spinal fusion surgery for neuromuscular scoliosis from November 2009 through September 2012.RESULTSWe analyzed data for 214 patients aged 1 to 20 years (median, 13 years), of whom 155 (72%) received at least 1 specific preoperative recommendation, whereas 59 patients (28%) were cleared for surgery without specific recommendations. Underlying diagnosis (P = 0.024), nonambulatory status (odds ratio [OR]: 2.02, 95% confidence interval [CI]: 1.09‐3.74), and increased number of preoperative medications (OR: 1.19, 95% CI: 1.06‐1.34) were statistically significantly associated with an increased rate of receiving preoperative recommendations from the hospitalist. Comorbidities such as seizure disorder (OR: 2.68, 95% CI: 1.29‐5.57) and gastrointestinal conditions (OR: 3.35, 95% CI: 1.74‐6.45) were also statistically significantly associated with specific presurgical recommendations being made by the pediatric hospitalist.CONCLUSIONA pediatric hospitalist preoperative program for children with neuromuscular scoliosis in anticipation of spinal fusion surgery is associated with a high rate of recommendations being made, especially in children with certain clinical characteristics. Journal of Hospital Medicine 2013;8:684–688. © 2013 Society of Hospital Medicine
Influence of landscape composition on northern bobwhite population response to field border establishment
Authors: Bowling Shannon A. , Moorman Christopher E. , Deperno Christopher S. , Gardner Beth 
Published: November 2013

ABSTRACTSince the 1960s, habitat loss resulting from cleaner farming, increased urbanization, and maturation of early successional cover has caused range‐wide decline of northern bobwhite (Colinus virginianus). Although field borders increase bobwhite habitat and increase local populations, understanding how the surrounding landscape influences bobwhite response to this management practice is critical to efficient implementation. We determined the relative influence of landscape composition and field border implementation on bobwhite densities and occupancy dynamics around crop fields in North Carolina and South Carolina, USA. We used 10‐minute distance point counts to estimate density, occupancy, colonization, and extinction rates of male bobwhite around 154 agriculture fields, half of which had a fallow field border. We estimated percent of cropland, forest, pasture, early successional, and urban cover within 1‐km radius buffers (314 ha) surrounding all point count locations. We examined the influence of 6 predictor variables (landscape composition metrics and field border presence) on bobwhite density and occupancy dynamics. Bobwhite density increased with the presence of field borders. Conversely, bobwhite density decreased as the percentage of urban, pasture, and forest lands increased. The presence of a field border did not influence occupancy, colonization, or extinction rates. However, as the percentage of cropland increased within the landscape, bobwhite occupancy increased and as the percentage of pasture increased, bobwhite colonization decreased. As the percentage of forest and urban increased and cropland decreased, bobwhite extinction rate increased. Our results indicated that local establishment of field borders does not increase bobwhite occupancy rates, but field borders can increase densities in suitable landscapes where bobwhite are present. Habitat restoration for bobwhite will most effectively increase population densities if focused in landscapes dominated by suitable cover types, where bobwhite occurrence is high. © 2013 The Wildlife Society.
Genome‐wide analysis of DNA copy number alterations and loss of heterozygosity in intracranial germ cell tumors
Authors: Terashima Keita , Yu Alexander , Chow Wing‐Yuk T. , Hsu Wei‐chun J. , Chen Peikai , Wong Stephen , Hung Yeung Sam , Suzuki Tomonari , Nishikawa Ryo , Matsutani Masao , Nakamura Hideo , Ng Ho‐Keung , Allen Jeffrey C. , Aldape Kenneth D. , Su Jack M. , Adesina Adekunle M. , Leung Hon‐chiu E. , Man Tsz‐Kwong , Lau Ching C. 
Published: November 2013

AbstractBackgroundsIntracranial germ cell tumors (GCTs) are rare and heterogeneous with very little is known about their pathogenesis and underlying genetic abnormalities.ProceduresIn order to identify candidate genes and pathways which are involved in the pathogenesis of these tumors, we have profiled 62 intracranial GCTs for DNA copy number alterations (CNAs) and loss of heterozygosity (LOH) by using single nucleotide polymorphism (SNP) array and quantitative real time PCR (qPCR).ResultsInitially 27 cases of tumor tissues with matched blood samples were fully analyzed by SNP microarray and qPCR. Statistical analysis using the genomic identification of significant targets in cancer (GISTIC) tool identified 10 regions of significant copy number gain and 11 regions of significant copy number loss. While overall pattern of genomic aberration was similar between germinoma and nongerminomatous germ cell tumors (NGGCTs), a few subtype‐specific peak regions were identified. Analysis by SNP array and qPCR was replicated using an independent cohort of 35 cases.ConclusionsFrequent aberrations of CCND2 (12p13) and RB1 (13q14) suggest that Cyclin/CDK‐RB‐E2F pathway might play a critical role in the pathogenesis of intracranial GCTs. Frequent gain of PRDM14 (8q13) implies that transcriptional regulation of primordial germ cell specification might be an important factor in the development of this tumor. Pediatr Blood Cancer 2014;61:593–600. © 2013 Wiley Periodicals, Inc.
Identification of novel NOTCH1 mutations: Increasing our knowledge of the NOTCH signaling pathway
Authors: Gallo Llorente L. , Luther H. , Schneppenheim R. , Zimmermann M. , Felice M. , Horstmann M.A. 
Published: November 2013

BackgroundAlterations in the NOTCH1 signaling pathway are found in about 60% of pediatric T‐ALL, but its impact on prognosis remains unclear.ProcedureWe extended the previously published CoALL cohort (n = 74) to a larger cohort (n = 127) and additionally included 38 Argentine patients from ALL IC‐BFM to potentially identify novel mutations and decipher a stronger discriminatory effect on the genotype/phenotype relationship with regard to early treatment response and long‐term outcome.ResultsOverall, 101 out of 165 (61.2%) T‐ALL samples revealed at least one NOTCH1 mutation, 28 of whom had combined NOTCH1 and FBXW7 mutations. Eight T‐ALL samples (4.8%) exclusively revealed FBXW7 mutations. Fifty‐six T‐ALL (33.9%) exhibited a wild‐type configuration of either gene. Four novel NOTCH1 mutations were identified localized in the C‐terminal PEST domain, in the rarely affected LNR repeat domain and in the ankyrin domain. Novel LNR mutations may contribute to a better understanding of the structure of the NOTCH1 negative regulatory region (NRR) and the R1946 mutation in the ankyrin domain may represent an unusual loss‐of‐function mutation.ConclusionsOverall, NOTCH1 pathway mutations did not affect the relapse rate and outcome of the extended T‐ALL cohort uniformly treated according to CoALL protocols, although NOTCH1 mutations were associated with good response to induction therapy (P = 0.009). Individually, HD and PEST domain mutations might exert distinct functional effects on cellular homeostasis under treatment NOTCH1 pathway activity with prognostic implications. Pediatr Blood Cancer 2014;61:788–796. © 2013 Wiley Periodicals, Inc.
Unbounded anonymous hierarchical IBE with continual‐key‐leakage tolerance
Authors: Zhang Mingwu , Yang Bo , Wang Chunzhi , Takagi Tsuyoshi 
Published: November 2013

ABSTRACTModern cryptographic schemes are constructed under the fundamental assumption that secret keys are perfectly hidden from all possible attackers. In practice, however, keys and internal states may partially be leaked. Recently, cryptographic construction with key‐leakage resilience has been a crucial research topic. In this work, we proposed an anonymous hierarchical identity‐based encryption that can tolerate partial leakage of secret keys. Our results were as follows. First, we provided a tolerance for continual key leakage that can capture both memory leakage and continual leakage. We extended a dual‐system encryption mechanism in orthogonal subgroups to achieve key‐leakage resilience and implicitly employed an update algorithm to guard against continual leakage. Second, the delegation depth is unbounded, which means that no predetermined depth was imposed in the setup algorithm, thus making the scheme very flexible in practice. We employed a secret‐sharing approach to split the master key into multiple shares in key components corresponding to the elements. Third, we analyzed and discussed the performance of allowable leakage‐tolerance bounds and the leakage rate of the proposed scheme and gave an evaluation that attains about 40–70% leakage rate under the Advanced Encryption Standard 112 security level. Copyright © 2013 John Wiley & Sons, Ltd.
Thick-shelled, grazer-protected diatoms decouple ocean carbon and silicon cycles in the iron-limited Antarctic Circumpolar Current
Authors: Assmy Philipp , Smetacek Victor , Montresor Marina , Klaas Christine , Henjes Joachim , Strass Volker H. , Arrieta Jesús M. , Bathmann Ulrich , Berg Gry M. , Breitbarth Eike , Cisewski Boris , Friedrichs Lars , Fuchs Nike , Herndl Gerhard J. , Jansen Sandra , Krägefsky Sören , Latasa Mikel , Peeken Ilka , Röttgers Rüdiger , Scharek Renate , Schüller Susanne E. , Steigenberger Sebastian , Webb Adrian , Wolf-Gladrow Dieter 
Published: November 2013

SignificanceSilica-shelled diatoms dominate marine phytoplankton blooms and play a key role in ocean ecology and the global carbon cycle. We show how differences in ecological traits of dominant Southern Ocean diatom species, observed during the in situ European Iron Fertilization Experiment (EIFEX), can influence ocean carbon and silicon cycles. We argue that the ecology of thick-shelled diatom species, selected for by heavy copepod grazing, sequesters silicon relative to other nutrients in the deep Southern Ocean and underlying sediments to the detriment of diatom growth elsewhere. This evolutionary arms race provides a framework to link ecology with biogeochemistry of the ocean.
Analysis of DNA methylation reveals a partial reprogramming of the Müller glia genome during retina regeneration
Authors: Powell Curtis , Grant Ana R. , Cornblath Eli , Goldman Daniel 
Published: November 2013

Significance
Retinal injury in zebrafish stimulates Müller glia (MG) to undergo a reprogramming event that transitions their identity from quiescent supportive cells to multipotent progenitors capable of repairing the damaged retina. Understanding the mechanisms underlying this reprogramming event may provide insights for stimulating retina regeneration in mammals. Here we report an epigenetic analysis of zebrafish MG, monitoring changes in their genomic methylation levels as they reprogram and generate progenitors. Although we find a changing DNA methylation landscape, our data also suggest that a basal methylation program may facilitate this transition. Interestingly, certain aspects of this basal methylation program are shared with mammals, suggesting they too may acquire progenitor properties under appropriate circumstances.
Genetic interplay betweenHLA-CandMIR148Ain HIV control and Crohn disease
Authors: Kulkarni Smita , Qi Ying , O’hUigin Colm , Pereyra Florencia , Ramsuran Veron , McLaren Paul , Fellay Jacques , Nelson George , Chen Haoyan , Liao Wilson , Bass Sara , Apps Richard , Gao Xiaojiang , Yuki Yuko , Lied Alexandra , Ganesan Anuradha , Hunt Peter W. , Deeks Steven G. , Wolinsky Steven , Walker Bruce D. , Carrington Mary 
Published: November 2013

SignificanceIn the human population differentHLA-Callotypes are present that have different expression levels at the cell surface. Individuals with higher expressedHLA-Callotypes demonstrate better HIV control but increased risk of Crohn disease. A microRNA, miR-148a, regulates expression of some HLA-C allotypes. We report here that this microRNA also varies in expression level between people.MIR148Avariation showed significant and opposing effects on HIV viral control vs. risk of Crohn disease, specifically in subjects withHLA-Calleles that are regulated by miR-148a. These results are independent of confounding effects by otherHLAloci because only HLA-C is regulated by miR-148a. Our data represent an example of gene interactions that affect immune response and thereby the risk of human disease.
Free-energy landscape of protein oligomerization from atomistic simulations
Authors: Barducci Alessandro , Bonomi Massimiliano , Prakash Meher K. , Parrinello Michele 
Published: November 2013

Significance
Oligomeric proteins, comprising two or more associating polypeptide chains, represent a large fraction of cellular proteins. In particular, many proteins self-associate into homooligomers to gain functional advantages. Our understanding of the oligomerization at the molecular level is currently limited because intermediates that occur in the process are short-lived in most occasions, precluding a direct experimental characterization. Using molecular dynamics simulations, enhanced by a sampling method developed in our group, we obtained an atomistic description of the assembly of an evolutionary-optimized trimeric protein. Our results are in excellent agreement with available experimental data and extend the current view of oligomerization by showing the importance of the apparently contradictory requirements of monomer preorganization and conformational flexibility.
X‐Ray Micro‐Computer Tomographic Method to Visualize the Microstructure of Different Apple Cultivars
Authors: Ting Valentina J.L. , Silcock Patrick , Bremer Phil J. , Biasioli Franco 
Published: November 2013

AbstractApples are appreciated for their texture with firmness acting as an indicator of quality. During prolonged storage, apples can soften and their texture can become undesirably mealy. Using an X‐ray microcomputer tomography (μ‐CT) scanner, the porosity (ratio of intercellular space [IS] to total volume) and the structural arrangement of the parenchyma tissue of 4 apple cultivars (Braeburn, Fuji, Golden Delicious, Jazz) stored under similar conditions for 100 d were visualized via the development of 2D and 3D images. The texture of the apples was also measured using a puncture test. The morphometric and textural measurements revealed that firm Jazz apples (flesh firmness: 29.84N) had a lower porosity (17%) compared to soft Golden Delicious apples (flesh firmness: 14.16N; porosity: 29.8%). In general, firm apples had a higher dry matter (%) and a lower porosity (%), while the reverse was true for softer apples. However, this was not an absolute trend as cultivar specific differences in the microstructural organization and consequent mechanical strength of the parenchyma tissue also influenced firmness. For example, although Fuji apples were firm (28.42N), they had a high porosity (29.3%) due to the presence of numerous small and compact IS. In comparison, soft Golden Delicious apples had a high porosity (29.8%) due to the presence of large, interconnected IS. Imaging technologies have the potential to provide a pictorial or graphical database showing the size range distribution of IS corresponding to different parenchyma tissue types and how they relate to apple texture and eating quality.
Soil structural indices' dependence on irrigation water quality and their association with chromophoric components in dissolved organic matter
Authors: Levy G. J. , Lordian A. , Goldstein D. , Borisover M. 
Published: November 2013

SummaryIrrigation with treated wastewater (TWW) may affect soil structure and stability and the characteristics of dissolved organic matter (DOM) of the soil solution. The objectives of our study were (i) to evaluate the impact of TWW irrigation, as compared with fresh water (FW) irrigation, on aggregate stability and saturated hydraulic conductivity (indices of soil structure stability) and (ii) to determine whether these indices can be associated with the chromophoric indicators of water‐extractable DOM in TWW‐ and FW‐irrigated soils. We studied aggregate stability and soil hydraulic conductivity (HC) of four different soil types irrigated with either TWW (for at least 5 years) or FW. The results were linked to earlier published data on the concentration scores of fluorescent chromophoric DOM components (obtained from excitation‐emission matrices of flouorescence coupled with parallel factor analysis), dissolved organic carbon (DOC) concentration and absorbance at 254 nm (Abs254). These were all obtained from water extracts of the same soils as those used in the current study. Irrigation with TWW decreased aggregate stability, in comparison to irrigation with FW, in the sandy clay and clay soils, while in the loamy sand TWW increased aggregate stability. The apparent steady state HCs in the TWW‐irrigated samples in the loamy sand, sandy clay and clay soils were similar to, or significantly less than, those obtained in the FW‐irrigated samples. In the sandy loam the opposite trend was noted. Results of principal component and classification analyses showed that the aggregate stability indices were directly associated with soil organic matter and DOM attributes in the coarse‐textured soils, while in the fine‐textured soils inverse associations were noted. Only in the fine‐textured soils were the HC attributes associated (directly) with some of the DOM characteristics. Our results suggest that structural indices of fine‐textured soils are more sensitive than those of coarse‐textured soils to the composition of water extractable DOM.
Importance of having agreeable friends in adolescence (especially when you are not)
Authors: Knack Jennifer M. , Jacquot Colette , Jensen‐Campbell Lauri A. , Malcolm Kenya T. 
Published: November 2013

AbstractUsing the actor–partner interdependence model, we examined whether adolescent's agreeableness, best friend's agreeableness, and the interaction between adolescent‐friend agreeableness are important for interpersonal functioning. Adolescents (N = 158) in fifth to eighth grades who were part of best friend pairs completed personality and friendship measures. Adolescents' adjustment and victimization experiences were assessed by peer nominations. For boys, best friend's agreeableness moderated the relationship between the target boy's agreeableness and overt victimization, relational victimization, and externalizing problems. For girls, best friend's agreeableness moderated the relationship between the target girl's agreeableness and internalizing problems and prosocial skills. This study provides an initial glimpse into how traits of friends can influence outcomes beyond what would be expected from adolescents' personality alone.