bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves
Authors: Salazar Marcos , Chérigo Lilia , Acosta Hildaura , Otero Rafael , Martínez-Luis Sergio 
Published: December 2014

Abstract
Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 μg of venom kg-1 b.m.) lowered the hemorrhagic activity of the venom alone in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L-1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to the isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HRMS and NMR (1H, 13C, HSQC, HMBC and COSY). A quercetrin concentration of 0.11 μmol L-1 prolonged the plasma coagulation time 2.6 times demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.
Finite nucleus effects, relativistic effects and picture change error in the IOTC/DKH2 contact spin densities of Cu, Ag, Au atoms
Authors: Malček Michal , Bučinský Lukáš , Biskupič Stanislav 
Published: December 2014

Abstract
Sensitivity of contact spin density as well as electron density to the size of nucleus is investigated using the Gaussian model of nucleus and the point charge nucleus model. Scalar Infinite Order Two Component and scalar second order Douglas-Kroll-Hess quasirelativistic contact spin densities (spin densities at the nucleus) of Cu, Ag and Au atoms are considered. The non-relativistic contact spin densities and the valence s-orbital contact densities of Kramers restricted orbitals (Cu, Ag, Au atoms) are presented as well. The picture change error in the quasirelativistic calculations of spin densities is corrected by analytic means. Uncontracted triple-zeta UTZ+Ns basis sets are employed, where N is the number of additional tight s Gaussians. In addition, the impact of tight p and d Gaussians is briefly discussed.
New inhibitors of glucosylceramide synthase and their effect on cell fate
Authors: Turáková Katarína , Lakatoš Boris , Ďuriš Andrej , Moravčíková Daniela , Berkeš Dušan 
Published: December 2014

Abstract
Glucosylceramide (GlcCer) is an essential glycosylated lipid found in organisms ranging from fungi to mammals. It is composed of a hydrophilic β-linked glucose and a hydrophobic ceramide, with a predominant content of sphingosine in mammals (d18:1). GlcCer is the precursor of a large scale of different glycosphingolipids. This cerebrozide is synthesized from uridine diphosphate-glucose and ceramide by a GlcCer synthase (UDP-glucose:ceramide glucosyltransferase; UGCG, EC 2.4.1.80). GlcCer-based sphingolipids have been identified as important mediators of a variety of cellular functions and their disequilibrium leads to pathological process development and may induce several diseases progression. Therefore, design of UGCG inhibitor represents an important topic for pharmaceutical research. In this paper, we aimed to study effects of newly synthesized derivatives of (±)-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP, known UGCG inhibitor) on: i) activity of UGCG in vitro; ii) thymocytes viability; iii) calcium transport through plasma membrane of thymocytes; iv) induction of apoptosis and autophagy in thymocytes. Thymocytes were isolated from thymus of three to seven weeks old mice (ICR strain). The key factors influencing the effect of PPMP analogues were their concentration, chemical structure and incubation time. Derivatives were able to change Ca2+ transport already after 15 min of cultivation, but their effects on cell viability were manifested at least after 12 h of cultivation. Four from fifteen studied compounds affected UGCG activity after four hour lasting cultivation, - but without correlation with data relating to effects on calcium transport and/or cell viability. Most potent UGCG inhibitor was chosen and applied for induction of apoptosis and autophagy in thymocytes. This inhibitor induced typical DNA fragmentation and upregulation of LC3B protein as autophagy marker, after 2 h and 4 h cultivation, respectively.
Analytical methods in herpesvirus genomics
Authors: Blaškovičová Jana , Labuda Ján 
Published: December 2014

Abstract
Genomics is a branch of bioanalytical chemistry characterized as the study of the genome structure and function. Genome represents the complete set of chromosomal and extrachromosomal genes of an organism, a cell, an organelle or a virus. There are at least five from eight species of herpesviruses commonly widespread among humans, Herpes simplex virus type 1 and 2, Varicella zoster virus, Epstein-Barr virus and Cytomegalovirus. Human gammaherpesviruses can cause serious diseases including B-cell lymphoma and Kaposi’s sarcoma. Diagnostics and study of the herpesviruses is directly dependent on the development of modern analytical methods able to detect and determine the presence and evolution of herpesviral particles/ genomes. Diagnostics and genomic characterization of human herpesvirus species is based on bioanalytical methods such as polymerase chain reaction (PCR), DNA sequencing, gel electrophoresis, blotting and others. The progress in analytical approaches in the herpesvirus genomics is reviewed in this article.
Evaluation of the Fertilizer Granules Strength Obtained from Plate Granulation with Different Angle of Granulation Blade
Authors: Leszczuk Tomasz 
Published: December 2014

Abstract
The goal of the paper was to assess the strength of the fertilizer granules obtained by non-pressure granulation method. The granulation process was carried out in plate granulator, according to the three-level experiment plan. A mixture of raw materials prepared in a Polish factory of agrochemicals for agriculture and horticulture was used as a study material and water was used as a wetting liquid. Granulator design parameters and process parameters were treated as entrance sizes of the experiment. Three different angles of granulation blade were used in experiments. This paper presents: the results of study of equivalent diameter size and the impact of changes in the angle of granulating blade on the strength of obtained granulate. Pfost apparatus and a set of sieves used in granulometric sieve were utilized in this study. A relation was suggested P∞ = f(α, ϰ, n, ww, t). The results were presented in the form of graphs and tables. Conclusions were presented.
Doubly Periodic Cracks in the Anisotropic Medium with the Account of Contact of Their Faces
Authors: Maksymovych Olesya , Pasternak Iaroslav , Sulym Heorhiy , Kutsyk Serhiy 
Published: December 2014

Abstract
The paper presents complex variable integral formulae and singular boundary integral equations for doubly periodic cracks in anisotropic elastic medium. It utilizes the numerical solution procedure, which accounts for the contact of crack faces and produce accurate results for SIF evaluation. It is shown that the account of contact effects significantly influence the SIF of doubly periodic curvilinear cracks both for isotropic and anisotropic materials.
Minimum Energy Control of 2D Positive Continuous-Discrete Linear Systems
Authors: Kaczorek Tadeusz 
Published: December 2014

Abstract
The minimum energy control problem for the 2D positive continuous-discrete linear systems is formulated and solved. Necessary and sufficient conditions for the reachability at the point of the systems are given. Sufficient conditions for the existence of solution to the problem are established. It is shown that if the system is reachable then there exists an optimal input that steers the state from zero boundary conditions to given final state and minimizing the performance index for only one step (q = 1). A procedure for solving of the problem is proposed and illustrated by a numerical example.
Temperature Relations of Selected Engine Oils Dynamic Viscosity
Authors: Hlaváč Peter , Božiková Monika , Presová Radmila 
Published: December 2014

Abstract
This article focuses on temperature relations of dynamic viscosity for selected engine oils. The effect of temperature on new and used oil dynamic viscosity was investigated. Measurements were performed on three different motor oil samples. All the three motor oil samples were synthetic. The first oil sample was new, the second sample was used for 15,000 km, and the third sample was used for 30,000 km. There were made two measurements of samples in one week. Dynamic viscosity was measured using a digital rotational viscometer Anton Paar DV-3P. The principle of measurement is based on the dependence of sample resistance to probe rotation. The results of measurements are shown as graphical relationships between dynamic viscosity and temperature. Decreasing exponential functions in temperature relationships were used for all the samples. The highest difference between the first and second measurement was observed in the new oil, and very small differences were found in other oils. Due to different types of oils and different stage of usage, the results could not be compared.
Study on toxic metal levels in commercial marine organisms from Romanian market
Authors: Amariei Sonia , Gutt Gheorghe , Oroian Mircea , Bodnar Alexandra 
Published: December 2014

Abstract
The goal of this study was to analyze the content of heavy metals in fish, shellfish, molluscs cephalopods on the Romanian market. We have analyzed heavy metals traces in sixteen marine organisms species. Analyses of heavy metal traces were done with mass spectrometer with inductively coupled plasma- ICP-MS, Agilent Technologies 7500 Series. Among the elements identified it was analyzed the content of Cd, Hg, Pb, metals with high toxicity to the human body, and the obtained values were interpreted in accordance with Commission Regulation (EC) No 1881/2006 concerning the maximum allowable quantity per kg wet product and Provisional Tolerated Weekly Intake (PTWI) established by the Joint Committee experts WHO / FAO. The risk assessment performed indicated that marine organisms were safe for the consumer except the four species that cadmium content may be exceeded for a regular or excessive consumption.
Prediction of products yield at the thermal cracking of vegetable oil
Authors: Cioroiu Doinita Roxana , Koncsag Claudia Irina 
Published: December 2014

Abstract
According to previous studies on the pyrolysis of vegetable oils, it resulted that the thermal cracking process is prone to produce large yields of ethylene, propylene, hydrogen and methane, comparable with the gas proceeding from the steam cracking of naphtha, but at much lower process temperature, this ensuring important energy savings. The studies are performed on very different raw materials and different reaction conditions, that being why at this moment it is very difficult to predict the products yield. This paper uses an analytical semiempirical model (ASEM) developed at the University of Florida, by applying it to a different raw material. The ASEM model fits very well to our experimental data, obtained at higher temperature but some parameters have to be adjusted. In the end we confirm a set of systemic parameters to be used for the prediction of main products yield proceeding from vegetable oil in an extended range of temperatures.
Functional Textiles – From Research and Development to Innovations and Industrial Uptake
Authors: Kiekens Paul , Van der Burght Els , Kny Erich , Uyar Tamer , Milašius Rimvydas 
Published: December 2014

Abstract
Functional textiles are one of the most important fields in textile industry and textile materials science. They include breathable, heat and cold-resistant materials, ultra-strong fabrics (e.g. as reinforcement for composites), new flameretardant fabrics (e.g. intumescent materials), optimisation of textile fabrics for acoustic properties, etc. Functional textiles became more and more important materials for various applications and interest in them grew year by year; and more and more conferences are focused on functional textiles, as well as the events which are not only textile conferences but encompass various fields of Material Science. This paper presents a short overview about the European Materials Research Society 2014 Fall meeting conference Symposium M “Functional textiles - from research and development to innovations and industrial uptake” and the projects which participated as symposium co-organisers: the European Coordination Action 2BFUNTEX funded by the EC 7th Framework Programme NMP, the COST Action MP1105 on “Sustainable flame retardancy for textiles and related materials based on nanoparticles substituting conventional chemicals (FLARETEX)” and the COST Action MP1206 on “Electrospun Nano-fibres for bio inspired composite materials and innovative industrial applications”.
An alternative methodology for imputing missing data in trials with genotype-by-environment interaction: some new aspects
Authors: Arciniegas-Alarcón Sergio , García-Peña Marisol , Krzanowski Wojtek Janusz , Santos Dias Carlos Tadeu dos 
Published: December 2014

Abstract
A common problem in multi-environment trials arises when some genotypeby- environment combinations are missing. In Arciniegas-Alarcón et al. (2010) we outlined a method of data imputation to estimate the missing values, the computational algorithm for which was a mixture of regression and lower-rank approximation of a matrix based on its singular value decomposition (SVD). In the present paper we provide two extensions to this methodology, by including weights chosen by cross-validation and allowing multiple as well as simple imputation. The three methods are assessed and compared in a simulation study, using a complete set of real data in which values are deleted randomly at different rates. The quality of the imputations is evaluated using three measures: the Procrustes statistic, the squared correlation between matrices and the normalised root mean squared error between these estimates and the true observed values. None of the methods makes any distributional or structural assumptions, and all of them can be used for any pattern or mechanism of the missing values.
Presence of specific antibodies and proviral DNA of small ruminant lentiviruses in lambs in their first weeks of life
Authors: Olech Monika , Kubiś Piotr , Lipecka Czesława , Junkuszew Andrzej , Gruszecki Tomasz M. , Kuźmak Jacek 
Published: December 2014

Abstract
The aim of this study was to investigate the presence of proviral DNA and colostral antibodies in lambs born to and fed by ewes infected with small ruminant lentiviruses (SRLV). It was demonstrated that all 20 lambs tested 24 h after colostrum ingestion were serologically positive with high antibody titres. These gradually decreased with time, and at week 12 all lambs were seronegative. Twenty percent of lambs tested at the 2nd week postpartum were provirus positive by qPCR as a result of consumption of infected colostrum or in utero infection. When tested at three months of life, 95% of the lambs were provirus positive, probably as a result of horizontal transmission of the virus. Since these animals could play an important role in the early propagation of SRLV to susceptible herdmates, early removal of provirus-positive animals could help to prevent new infections.
Outbreak of duck viral hepatitis in duckling flocks in Poland - case report
Authors: Kozdru Wojciech , Czekaj Hanna , Lorek Mariusz 
Published: December 2014

Abstract
The aim of the study was to determine the aetiologic agent causing deaths in two flocks of Pekin ducklings at the age of 12 d in Poland. on the basis of clinical symptoms and pathological changes, viral hepatitis infection was suspected in the birds. During the necropsy, liver sections were collected, from which total cellular RNA was isolated. Then, real-time polymerase chain reaction (RT-PCR) was performed using primers complementary to the pre-S region of the duck hepatitis virus genome. In all liver samples, the presence of a 530 bp PCR product was detected. The RT-PCR demonstrated the presence of genetic material of duck hepatitis virus type 1 (DH type 1) in the examined ducklings.
Infections of swine caused by Leptospira serovars of serogroup Sejroe - possibilities of recognition with the use of PCR
Authors: Wasiński Bernard 
Published: December 2014

Abstract
The aim of this study was to confirm the presence of leptospires from the Sejroe serogroup in aborting seropositive sows and to identify which serovars of this serogroup are responsible for the infection. Serum and urine samples from 20 aborting sows, reared in five farms infected with the Sejroe serogroup, were submitted for examination. Serum samples were examined by the microscopic agglutination test (MAT) and urine samples by polymerase chain reaction (PCR) with primer sets specific for genus Leptospira, species L. borgpetersenii, the Sejroe serogroup, and the Hardjo-bovis genotype. All the examined serum samples showed titres specific to the Sejroe serogroup, ranging from 100 to 6400. PCR indicated the presence of Leptospira genus-specific DNA sequences in all urine samples examined. DNA sequences specific for both L. borgpetersenii and the Sejroe serogroup were found in 12 of these samples. Additionally, the sequences specific only for L. borgpetersenii were found in five urine samples and sequences for the Sejroe serogroup in two samples. The DNA sequence specific for the Hardjo-bovis genotype was not found in the urine samples. PCR confirmed the results of the MAT, displayed the presence of Leptospira sp. DNA in the urine of all the sows examined, proved the affinity of the detected leptospires to the Sejroe serogroup or at least to L. borgpetersenii, and excluded the Hardjo serovar as the reason for the infections described in the sows.
Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction
Authors: Khamesipour Faham , Doosti Abbas , Emadi Mohsen Fard , Awosile Babafela 
Published: December 2014

Abstract
The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P < 0.0001; χ2 = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.
Application of quantitative PCR for detection of Mycoplasma suis in blood samples
Authors: Jabłoński Artur , Borowska Dominika , Zębek Sylwia , Kowalczyk Andrzej , Dors Arkadiusz , Żmudzki Jacek , Nowak Agnieszka , Pejsak Zygmunt 
Published: December 2014

Abstract
The aim of the study was to develop and validate a real-time PCR method, using a TaqMan probe, for quantification of Mycoplasma suis in porcine blood. No PCR signals with closely related non-haemotrophic mycoplasmas were obtained. The detection limit of PCR for plasmid combined with blood DNA was determined to be 103/reaction (5 μL of DNA) (1.2x105 target copies in 1 mL of blood). The linearity of real-time PCR (near 1) indicates its use as a quantitative method. Real-time and quantitative PCR were sensitive and specific for the detection and quantification of M. suis in the blood of animals with acute and chronic form of eperythrozoonosis. Developed quantitative PCR cannot be used to detect carrier animals with a small amount of M. suis in their blood. The validity of real-time PCR used in the studies was confirmed by the low inter- and intra-assay coefficients of variation. This fact confirms the applicability of the assay in other laboratories.
Occurrence of Campylobacter on carcasses of slaughtered animals between 2009 and 2013
Authors: Wieczorek Kinga , Osek Jacek 
Published: December 2014

Abstract
A total of 2668 swabs from poultry (n = 2166), pig (n = 311), and cattle (n = 191) carcasses were collected in slaughterhouses all over Poland and tested for the presence of Campylobacter. It was found that 1319 (49.4%) of them were contaminated with these bacteria. The percentages of the positive samples were different in each year of the study and the highest proportion of Campylobacter contaminated samples occurred in 2009, when 64.1% of investigated carcasses were positive. On the other hand, the lowest prevalence of Campylobacter was observed in 2013, in the last year of the survey. In all kind of carcass samples both C. jejuni and C. coli were identified, although the pork meat was more contaminated with C. coli (75.3% of positive samples) than with C. jejuni (24.7%), whereas poultry was nearly equally positive for C. jejuni and C. coli (50.6% and 49.4% respectively). The analysis of seasonal contamination of the carcasses revealed that more positive results were found during the second half of year than between January and June. The prevalence of Campylobacter showed that in all provinces, except one (Pomorskie), the mean percentage of the positive samples was above 40%. The most contaminated samples were identified in Lubelskie (69.3%) and Zachodniopomorskie (66.3%) regions. The obtained results showed that slaughtered animals in Poland, especially broilers, were often contaminated with Campylobacter, either C. jejuni or C. coli.
Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland
Authors: Korpysa-Dzirba Weronika , Osek Jacek 
Published: December 2014

Abstract
The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.
Antipruritic application of ovocystatin in atopic dermatitis in dogs - preliminary study
Authors: Popiel Jarosław , Cekiera Agnieszka , Kurosad Agnieszka , Pasławska Urszula , Noszczyk-Nowak Agnieszka , Nicpo Józef 
Published: December 2014

Abstract
The study was an attempt to determine the possibilities of using ovocystatin, a component of a new generation product of natural origin, in local therapy of atopic dermatitis in dogs by suppressing pruritus during illness. Chicken egg cystatin was used locally in the interdigital spaces of forelimbs of dogs used in the experiment. The degree of pruritus and clinical changes in the animals were defined using CADESI-03 scale before and after the beginning of the experiment. The results obtained proved that ovocystatin may be used as a substance suppressing pruritus in atopic dermatitis.