bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mindray BC‐6800 body fluid mode, performance of nucleated cells, and differential count in ascitic and pleural fluids
Authors: Buoro S. , Mecca T. , Azzarà G. , Seghezzi M. , Candiago E. , Gianatti A. , Crippa A. , La Gioia A. 
Published: November 2015

SummaryIntroductionAn accurate and rapid analysis of cells in body fluids (BFs) is important for diagnosis and follow‐up in many pathological conditions. We evaluated the analytical performance of the module BF Mindray BC‐6800 (BC‐6800‐BF) for cytometric analysis of ascitic and pleural fluids.MethodsA total of 99 ascitic and 45 pleural samples were collected and assessed with BC‐6800‐BF and optical microscopy. This study also includes the evaluation of limit blank (LoB), limit detection (LoD), limit quantitation, (LoQ), carryover, linearity, and diagnostic concordance between the two methods.ResultsFor TC‐BF, LoB was 1 × 106 cells/L, LoD was 3 × 106 cells/L, and LoQ was 4 × 106 cells/L. Linearity was excellent (r2 = 0.99) and carryover was negligible. TC‐BF performed with the two methods showed Pearson's correlation of 0.99 (P < 0.0001), Passing–Bablok regression y = 1.04x − 1.17, and bias 33.7 cells. In ascitic fluids, polymorphonuclear cells (PMN) showed an area under curve (AUC) of 0.98 (P < 0.0001). In pleural fluids, mononuclear cells (MN) and PMN % displayed an AUC of 0.79 (P < 0.0001) and 0.93 (P < 0.0001), respectively.ConclusionsBC‐6800‐BF in ascitic and pleural fluids offers rapid and accurate cell and differential counts in clinically relevant concentration ranges. The use of BC‐6800‐BF may allow to replace routine optical counting, except for samples displaying abnormal cell counts or abnormal DIFF scattergram.
Management of Indigenous patients presenting with non ST‐segment elevation acute coronary syndrome in South Australia: a retrospective cohort study
Authors: Roe Y. L. , Esterman A. , McDermott R. , Zeitz C. 
Published: November 2015

AimUsing Australian guidelines for management of acute coronary syndromes, we assessed the probability of an Indigenous patient receiving interventional and therapeutic care after presenting in two metropolitan hospitals.MethodsA retrospective case note review of patients admitted through two Adelaide public tertiary hospital emergency departments from December 2007 to December 2009. The study cohort was 488 patients with high‐risk clinical features without ST‐segment elevation.ResultsIndigenous patients were significantly younger, present later in the disease process and have a higher burden of cardiovascular risk factors when compared with non‐Indigenous patients. Indigenous patients were 54% more likely to receive angiography (Risk ratios (RR) = 1.54; 95% CI 1.31; 1.81) than non‐Indigenous patients, however, this difference disappeared after adjustment for age, sex and propensity score. Indigenous patients were 20% more likely to receive the recommended medications (RR = 1.19, 95% CI 1.01; 1.40) compared with non‐Indigenous patients. Patients over 65 years were 53% less likely to receive an angiogram (RR = 0.47, 95% CI 0.38; 0.56) and were 35% less likely to receive the recommended medications (RR = 0.65, 95% CI 0.54; 0.78) than a patient at the ages of 18–49. Women were almost 20% less likely to receive an angiogram (RR = 0.81, 95% CI 0.66; 0.99) and 20% less likely to receive the recommended medications (RR = 0.80, 95% CI 0.71; 0.91) when compared with men. The likelihood of receiving medications on discharge was significantly influenced by age, gender, ethnicity, comorbid burden and revascularisation.ConclusionsThe younger age and significantly higher‐risk profile of Indigenous adults presenting to SA hospitals with acute coronary syndromes appears to lead to different management decisions, which may well be led by patient factors. Many of these risk conditions can be better managed in the primary care setting.
Adhesive systems for restoring primary teeth: a systematic review and meta‐analysis of in vitro studies
Authors: Lenzi Tathiane Larissa , Gimenez Thais , Tedesco Tamara Kerber , Mendes Fausto Medeiros , Rocha Rachel de Oliveira , Raggio Daniela Prócida 
Published: November 2015

AimTo systematically review the literature for in vitro studies that evaluated the immediate or after ageing bond strength of etch‐and‐rinse and self‐etch adhesive systems to enamel and dentin of primary teeth.DesignThe search was conducted in PubMed/MEDLINE, Cochrane, SciELO, Lilacs, and Scopus databases with no publication year or language limits, following the preferred reporting items for systematic reviews and meta‐analyses (PRISMA) statement. From 459 potentially eligible studies, 39 were selected for full‐text analysis, and 5 were identified in reference lists, with 36 considered in the meta‐analysis. Two reviewers independently selected the studies, extracted the data, and assessed the risk of bias. Pooling bond strength data was calculated using random effects analysis method, comparing two categories of adhesives (etch‐and‐rinse versus self‐etch systems) when applied in different types and conditions of substrate (α = 0.05).ResultsNo statistical significant difference in bond strength between both categories was observed in caries‐affected dentin at immediate evaluation and in sound dentin after ageing. Etch‐and‐rinse adhesives, however, performed better in sound enamel and dentin substrates considering immediate bond strength. None study assessed the long‐term adhesive effectiveness to sound or demineralized enamel.ConclusionAlthough the articles included in this meta‐analysis showed high heterogeneity and high risk of bias, the in vitro literature suggests superior performance of etch‐and‐rinse adhesives in primary teeth in comparison with self‐etch systems.
Expression of inflammatory cytokines and MMPs on replanted teeth at different extra‐alveolar time: an ex vivo and in vivo study
Authors: Ahn Hyo Jung , Nam Ok Hyung , Lee Hyo‐Seol , Kim Eun‐Cheol , Cohenca Nestor , Choi Sung Chul 
Published: November 2015

BackgroundImmediately after the avulsed tooth is replanted, a complex inflammatory response ensues. As part of the periodontium healing process, the extracellular matrix macromolecules are essential to create the cellular environment required during healing and morphogenesis.AimThis study was designed to evaluate the correlation between different extra‐alveolar dry times and inflammatory cytokines and matrix metalloproteinases (MMPs) as part of the periodontal ligament (PDL) gene expression.DesignThe first phase of the study aimed testing human PDL cells ex vivo. Extracted teeth were dried for 15 and 30 min. The PDL cells were extracted and analyzed by qRT‐PCR. The second phase was performed in vivo, and 36 Sprague Dawley rat first maxillary molars were extracted and replanted after 15, 30, and 60 min extra‐alveolar time. We tested the levels of inflammatory cytokines and MMPS in periodontal tissue at 3, 7, and 28 days after tooth replantation. The replanted area was dissected, grounded, and analyzed by RT‐PCR.ResultsExpressions of IL‐1β, IL‐6, TNF‐α, and MMP‐3 and MMP‐9 were significantly higher in the replanted teeth. Extended dry time had a direct correlation with induction of pro‐inflammatory cytokine and MMPs in PDL cells.ConclusionOur study showed that pro‐inflammatory cytokines were more significantly expressed in the tissues surrounding the replanted teeth. Future research must be undertaken to additionally confirm the release of these cytokines and be focused on the inhibition of these cytokines to reduce inflammation of replanted teeth.
A population‐based estimate of the economic burden of influenza in Peru, 2009–2010
Authors: Tinoco Yeny O. , Azziz‐Baumgartner Eduardo , Rázuri Hugo , Kasper Matthew R. , Romero Candice , Ortiz Ernesto , Gomez Jorge , Widdowson Marc‐Alain , Uyeki Timothy M. , Gilman Robert H. , Bausch Daniel G. , Montgomery Joel M. ,   the Peru Influenza Cohorts Working Group
Published: November 2015

IntroductionInfluenza disease burden and economic impact data are needed to assess the potential value of interventions. Such information is limited from resource‐limited settings. We therefore studied the cost of influenza in Peru.MethodsWe used data collected during June 2009–December 2010 from laboratory‐confirmed influenza cases identified through a household cohort in Peru. We determined the self‐reported direct and indirect costs of self‐treatment, outpatient care, emergency ward care, and hospitalizations through standardized questionnaires. We recorded costs accrued 15‐day from illness onset. Direct costs represented medication, consultation, diagnostic fees, and health‐related expenses such as transportation and phone calls. Indirect costs represented lost productivity during days of illness by both cases and caregivers. We estimated the annual economic cost and the impact of a case of influenza on a household.ResultsThere were 1321 confirmed influenza cases, of which 47% sought health care. Participants with confirmed influenza illness paid a median of $13 [interquartile range (IQR) 5–26] for self‐treatment, $19 (IQR 9–34) for ambulatory non‐medical attended illness, $29 (IQR 14–51) for ambulatory medical attended illness, and $171 (IQR 113–258) for hospitalizations. Overall, the projected national cost of an influenza illness was $83–$85 millions. Costs per influenza illness represented 14% of the monthly household income of the lowest income quartile (compared to 3% of the highest quartile).ConclusionInfluenza virus infection causes an important economic burden, particularly among the poorest families and those hospitalized. Prevention strategies such as annual influenza vaccination program targeting SAGE population at risk could reduce the overall economic impact of seasonal influenza.
Warmth and competence in animals
Authors: Sevillano Verónica , Fiske Susan T. 
Published: November 2015

AbstractSocial‐perception dimensions may explain human‐animal relationships because animals show intent toward humans (social perception's warmth dimension) and, consequently, their potential effect on humans is relevant (competence dimension). After reviewing current literature about perceptions of animals’ ascribed intentions and abilities, three studies tested the Stereotype Content Model (SCM) (Fiske, Cuddy, Glick, & Xu, 2002) and the Behaviors from Intergroup Affect and Stereotypes Map (Cuddy, Fiske, & Glick, 2007) regarding animal targets. Study 1 found a four‐cluster SCM structure. Warmth and competence judgments predicted specific emotions and behavioral tendencies toward animals (Study 2). Study 3 supported associations between animals and social groups based on their respective perceived warmth and competence. Taken together, results showed the relevance of SCM dimensions for social perception of animals.
Shaping species with ephemeral boundaries: the distribution and genetic structure of desert tortoise (Gopherus morafkai) in the Sonoran Desert region
Authors: Edwards Taylor , Vaughn Mercy , Rosen Philip C. , Meléndez Torres Cristina , Karl Alice E. , Culver Melanie , Murphy Robert W. 
Published: November 2015

AbstractAimWe examine the role biogeographical features played in the evolution of Morafka's desert tortoise (Gopherus morafkai) and test the hypothesis thatG. morafkaimaintains genetically distinct lineages associated with different Sonoran Desert biomes. Increased knowledge of the past and present distribution of the Sonoran Desert region's biota provides insight into the forces that drive and maintain its biodiversity.LocationSonoran Desert biogeographical region; Sonora and Sinaloa, Mexico and Arizona, USA.MethodsWe examined wild tortoises from Mexico (n = 155) and Arizona (n = 78), spanning their known distribution. We used mtDNAsequences to reconstruct matrilineal relationships and 25 microsatellite (STR) loci for Bayesian analyses of gene flow. We performed clinal analyses on both mtDNAandSTRloci to determine the position and amount of introgression where lineages co‐occur. We usedGISto assess the association of genetic structuring with ecological features. We used these data in a hypothesis‐driven approach to assess different models of how genetic diversity is maintained and distributed inG. morafkai.ResultsGopherus morafkaiwas found to comprise genetically and geographically distinct ‘Sonoran’ and ‘Sinaloan’ lineages. Both lineages occurred in a relatively narrow zone of overlap in Sinaloan thornscrub, where it transitions into Sonoran desertscrub. Limited introgression occurred at the contact zone. The best‐fit model suggests that these lineages diverged in parapatry where the distribution of genotypes is environment‐dependent and introgression is inhibited by exogenous selection.Main conclusionsThe historically shifting ecotone between tropical deciduous forest and Sonoran desertscrub appears to be a boundary that fostered divergence between parapatric lineages of tortoises. The sharp genetic cline between the two lineages suggests that periods of isolation in temporary refugia due to Pleistocene climatic cycling influenced divergence. Despite incomplete reproductive isolation, the Sonoran and Sinaloan lineages ofG. morafkaiare on separate evolutionary trajectories.
A meta‐analysis of XPC Lys939Gln polymorphism and melanoma susceptibility
Authors: Jiang W. , Zhang H. , Chen Q.W. , Xie S. 
Published: November 2015

AbstractBackgroundIt has been reported that polymorphisms of XPC Lys939Gln may affect the risk of melanom. However, the results have been inconsistent.We performed a comprehensive meta‐analysis to determine the association between XPC Lys939Gln polymorphism and melanoma susceptibility.MethodsBased on comprehensive searches of the MEDLINE, EMBASE and ISI Web of knowledge, China National Knowledge Infrastructure (CNKI) and Wanfang Database, we identified eligible studies about the association between XPC Lys939Gln polymorphism and melanoma risk.ResultsA total of 4631 cases and 5111 controls in studies were included in this meta‐analysis. All studies were conducted in Caucasian populations. Allele model (Gln vs. Lys: P = 0.22; OR = 1.07, 95% CI = 0.96–1.18), and homozygous model (Gln/Gln vs. Lys/Lys: P = 0.66; OR = 1.03, 95% CI = 0.91–1.17) did not show increased risk of developing melanoma. Similarly, dominant model Gln/Gln and Gln/Lys vs. Lys/Lys: P = 0.07; OR = 1.17, 95% CI = 0.99–1.40) and recessive model (Gln/Gln vs. Gln/Lys and Lys/Lys: P = 0.67; OR = 1.03, 95% CI = 0.90–1.19) failed to show increased risk of developing melanoma.ConclusionOur pooled data suggest that there was no evidence for a major role of XPC Lys939Gln polymorphism in the pathogenesis of melanoma.
Collecting and Pooling Assessment and Outcome Data in Couple and Family Therapy Training Clinics: Reasons to Do It, Common Problems, and Some Suggestions for Avoiding Them
Authors: Wampler Karen S. , Bartle‐Haring Suzanne 
Published: November 2015

The article by Johnson et al. (2015) is an excellent example of many of the advantages and challenges of collecting data in clinics associated with Couple and Family Therapy training programs. In the hopes of encouraging more use of clinic datasets for research, we suggest some ways to improve the feasibility, quality, and value of routinely collecting such data.
Effects of periodontal treatment on inflammation and oxidative stress markers in patients with metabolic syndrome
Authors: Torumtay G. , Kırzıoğlu F. Y. , Öztürk Tonguç M. , Kale B. , Calapoğlu M. , Orhan H. 
Published: November 2015

Background and ObjectiveMetabolic syndrome (MetS) is a combination of risk factors (e.g. impaired glucose tolerance, hypertension, and dyslipidaemia) that significantly contribute to the development of cardiovascular diseases. The aim of the study was to compare the effects of nonsurgical periodontal treatment (NSPT) on inflammatory and oxidative stress markers in individuals with MetS and systemically healthy (SH) who were chronic periodontitis (CP).Material and MethodsA total of 50 patients with chronic periodontitis (25 with MetS and 25 SH) were included. Clinical periodontal measurements were recorded, and serum and whole‐saliva samples were collected from all patients at baseline, and 3 and 6 mo following NSPT. The levels of fasting plasma glucose, glycated haemoglobin (HbA1c), triglyceride (TRG), total cholesterol, high‐density lipoprotein cholesterol and low‐density lipoprotein cholesterol were analysed. The levels of high‐sensitivity C‐reactive protein (hs‐CRP), interleukin (IL)‐6 and IL‐10 were determined using ELISA kits, and total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI) levels were measured.ResultsAfter NSPT, significant and similar improvements of all periodontal parameters were observed in both groups compared with baseline measurements. There were decreases in the levels of serum hs‐CRP and IL‐6, whereas increases in serum IL‐10 were found in both groups, at all time points. Serum TOS and OSI showed no significant change in either group at any time point. Compared with the SH group, serum TAC levels were higher in the MetS group at baseline but lower at the 3‐mo time‐point. There was no difference in TAC levels between the groups at 6 mo. Saliva IL‐6 was higher in the MetS group than the SH group at all time points. The levels of IL‐6 and OSI in saliva decreased following NSPT in both groups, whereas salivary TAC concentrations increased. In the MetS group, TRG and HbA1c levels decreased significantly at 3 mo.ConclusionNSPT decreased oxidative stress and the inflammatory status of patients with MetS and chronic periodontitis. Although similar periodontal improvements were achieved in both groups, the decreases in levels of hs‐CRP and IL‐6 in the MetS group did not reach the levels in the SH group. Based on these results, NSPT could be more effective in the control of systemic inflammation in patients with MetS in the short‐term.
The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature‐induced periodontitis in rats
Authors: Çalışır M. , Akpınar A. , Poyraz Ö. , Göze F. , Çınar Z. 
Published: November 2015

BackgroundHumic acid is a soil extract found widely around the world. This product includes some trace elements important for human's health. The purpose of this study was to evaluate the morphometric and histopathological changes associated with an experimental periodontitis model in rats in response to systemic administration of humic acid.Material and MethodsThirty‐eight male Wistar rats were divided into five experimental groups: non‐ligated (NL, n = 6) group; ligature‐only (LO, n = 8) group; ligature + systemic administration of humic acid (20, 80 and 150 mg/kg body weight per day for 15 d respectively) (S‐20, S‐80 and S‐150) groups. 4/0 silk ligatures were placed at the gingival margin of lower first molars of the mandibular quadrant. The animals were killed at the end of 15 d. Changes in alveolar bone levels were clinically measured, using a stereomicroscope (× 25), as the distance from the cementoenamel junction to the alveolar bone crest. Tissues were histopathologically examined to assess the differences of osteoclast numbers, osteoblastic activity and inflammatory cell infiltration among the study groups. Enzyme‐linked immunosorbent assay interleukin (IL)‐1β and IL‐10 levels in serum and gingival homogenates were evaluated.ResultsAt the end of 15 d, the alveolar bone loss was significantly higher in the LO group compared to the NL, S‐80 and S‐150 groups (p < 0.05). In addition, the alveolar bone loss in the S‐80 group was significantly lower than the LO and S‐20 groups (p < 0.05). The osteoblastic activity in the S‐80 and S‐150 groups was significantly higher than the other groups (p < 0.05). The osteoclast number in the LO group was significantly higher than the NL, S‐80 and S‐150 groups (p < 0.05). Inflammatory cell infiltration was significantly higher in LO and S‐20 groups than the other groups (p < 0.05). The highest serum and gingival homogenate IL‐10 levels were determined in the S‐80 group (p < 0.05). The serum and gingival homogenate IL‐1β levels in the LO group were significantly higher than the other groups (p < 0.05). Both 80 and 150 mg/kg dosages of humic acid significantly reduced the periodontitis‐related bone loss and inflammation, but the differences between these two groups were not statistically significant (p > 0.05).ConclusionsWithin the limits of this study, it can be suggested that humic acid, when administered systemically as an 80 mg/kg dose, may prevent alveolar bone loss and reduce inflammation in the rat model.
Ankaferd blood stopper enhances healing after osseous grafting in patients with intrabony periodontal defects
Authors: Pamuk F. , Cetinkaya B. O. , Keles G. C. , Balli U. , Koyuncuoglu C. Z. , Cintan S. , Kantarci A. 
Published: November 2015

Background and ObjectiveThe aim of this clinical study were to compare the clinical efficacy of ankaferd blood stopper (ABS) when used in combination with autogenous cortical bone graft (ACB) in the treatment of intrabony periodontal defects.Material and MethodsThe study was planned as a split‐mouth design. Fifteen patients with chronic periodontitis at 30 sites (six men, nine women; 42 ± 7 years) were included. Treatment sites had probing pocket depths (PPD) of ≥ 6 mm and osseous defect depths of ≥ 4 mm as radiographically assessed. Following the initial periodontal therapy, patients were randomly assigned to two treatments in contralateral areas of the dentition: ACB + ABS or ACB alone. At baseline and 6 mo after surgery, clinical parameters of plaque index, gingival index, PPD, clinical attachment level and gingival recession (GR) were recorded. The primary outcome variable was the change in clinical attachment level between baseline and 24 wk after surgery. Gingival crevicular fluid samples were collected immediately before surgery and at 2, 4, 6, 12 and 24 wk after the surgery. Gingival crevicular fluid volume was calculated and vascular endothelial growth factor levels in gingival crevicular fluid were measured.ResultsPPD decreased, clinical attachment level improved and gingival index decreased significantly in response to both modes of treatment (p < 0.05). Both treatment modalities resulted in a significant gain in radiographic bone levels compared to baseline (p < 0.05). Intergroup comparisons showed that there was a significantly higher gain in clinical attachment level in the ABS/ACB group compared to ACB group (p < 0.05) with significantly less GR (p < 0.05). Similarly, vascular endothelial growth factor concentration in gingival crevicular fluid was significantly higher in the ABS/ACB group at postoperative weeks 2 and 4 compared to the ACB group (p < 0.01).ConclusionsThe findings suggest that ABS enhances the soft tissue healing during the periodontal defect fill by the ACB by stimulating angiogenesis and vascular endothelial cell function, prevents GR and thereby increases the clinical attachment gain.