bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflammation-Mediated Notch Signaling Skews Fanconi Anemia Hematopoietic Stem Cell Differentiation
Authors: Du Wei , Amarachintha Surya , Sipple Jared , Schick Jonathan , Steinbrecher Kris , Pang Qishen 
Published: August 2013

AbstractHematopoietic stem cells (HSCs) can either self-renew or differentiate into various types of cells of the blood lineage. Signaling pathways that regulate this choice of self-renewal versus differentiation are currently under extensive investigation. In this study, we report that deregulation of Notch signaling skews HSC differentiation in mouse models of Fanconi anemia (FA), a genetic disorder associated with bone marrow failure and progression to leukemia and other cancers. In mice expressing a transgenic Notch reporter, deletion of the Fanca or Fancc gene enhances Notch signaling in multipotential progenitors (MPPs), which is correlated with decreased phenotypic long-term HSCs and increased formation of MPP1 progenitors. Furthermore, we found an inverse correlation between Notch signaling and self-renewal capacity in FA hematopoietic stem and progenitor cells. Significantly, FA deficiency in MPPs deregulates a complex network of genes in the Notch and canonical NF-κB pathways. Genetic ablation or pharmacologic inhibition of NF-κB reduces Notch signaling in FA MPPs to near wild type level, and blocking either NF-κB or Notch signaling partially restores FA HSC quiescence and self-renewal capacity. These results suggest a functional crosstalk between Notch signaling and NF-κB pathway in regulation of HSC differentiation.
Melanoma Differentiation–Associated Gene 5 Senses Hepatitis B Virus and Activates Innate Immune Signaling To Suppress Virus Replication
Authors: Lu Hsin-Lin , Liao Fang 
Published: August 2013

Abstract
Retinoic acid–inducible gene-I (RIG-I) and melanoma differentiation–associated gene 5 (MDA5) belong to the RIG-I–like receptors family of pattern recognition receptors. Both RIG-I and MDA5 have been shown to recognize various viral RNAs, but whether they mediate hepatitis B virus (HBV) infection remains unclear. In this study, we demonstrated that the expression of MDA5, but not RIG-I, was increased in Huh7 cells transfected with the HBV replicative plasmid and in the livers of mice hydrodynamically injected with the HBV replicative plasmid. To further determine the effect of RIG-I–like receptors on HBV replication, we cotransfected the HBV replicative plasmid with RIG-I or MDA5 expression plasmid into Huh7 cells and found that MDA5, but not RIG-I at a similar protein level, significantly inhibited HBV replication. Knockdown of endogenous MDA5, but not RIG-I, in Huh7 cells transfected with the HBV replicative plasmid significantly increased HBV replication. Of particular interest, we found that MDA5, but not RIG-I, was able to associate with HBV-specific nucleic acids, suggesting that MDA5 may sense HBV. Finally, we performed in vivo experiments by hydrodynamic injection of the HBV replicative plasmid into wild-type, MDA5−/−, MDA5+/−, or RIG-I+/− mice, and found that MDA5−/− and MDA5+/− mice, but not RIG-I+/− mice, exhibited an increase of HBV replication as compared with wild-type mice. Collectively, our in vitro and in vivo studies both support a critical role for MDA5 in the innate immune response against HBV infection.
Peripheral Tissue Homing Receptors Enable T Cell Entry into Lymph Nodes and Affect the Anatomical Distribution of Memory Cells
Authors: Brinkman C. Colin , Rouhani Sherin J. , Srinivasan Nithya , Engelhard Victor H. 
Published: August 2013

AbstractPeripheral tissue homing receptors enable T cells to access inflamed nonlymphoid tissues. In this study, we show that two such molecules, E-selectin ligand and α4β1 integrin, enable activated and memory T cells to enter lymph nodes (LN) as well. This affects the quantitative and qualitative distribution of these cells among regional LN beds. CD8 memory T cells in LN that express these molecules were mostly CD62Llo and would normally be classified as effector memory cells. However, similar to central memory cells, they expanded upon Ag re-encounter. This led to differences in the magnitude of the recall response that depended on the route of immunization. These novel cells share properties of both central and effector memory cells and reside in LN based on previously undescribed mechanisms of entry.
Noncovalent Association of Protein and Capsular Polysaccharide on Bacteria-Sized Latex Beads as a Model for Polysaccharide-Specific Humoral Immunity to Intact Gram-Positive Extracellular Bacteria
Authors: Colino Jesus , Duke Leah , Snapper Clifford M. 
Published: August 2013

AbstractIntact Streptococcus pneumoniae expressing type 14 capsular polysaccharide (PPS14) and type III S. agalactiae containing a PPS14 core capsule identical to PPS14 exhibit noncovalent associations of PPS14 and bacterial protein, in contrast to soluble covalent conjugates of these respective Ags. Both bacteria and conjugates induce murine PPS14-specific IgG responses dependent on CD4+ T cells. Further, secondary immunization with conjugate and S. agalactiae, although not S. pneumoniae, results in a boosted response. However, in contrast to conjugate, PPS14-specific IgG responses to bacteria lack affinity maturation use the 44.1-idiotype and are dependent on marginal zone B cells. To better understand the mechanism underlying this dichotomy, we developed a minimal model of intact bacteria in which PPS14 and pneumococcal surface protein A (PspA) were stably attached to 1 μm (bacteria-sized) latex beads, but not directly linked to each other, in contrast to PPS14–PspA conjugate. Beads coated simultaneously with PPS14+[PspA], similar to conjugate, induced in mice boosted PPS14-specific IgG secondary responses, dependent on T cells and ICOS-dependent costimulation, and in which priming could be achieved with PspA alone. In contrast to conjugate, but similar to intact bacteria, the primary PPS14-specific IgG response to beads coated simultaneously with PPS14+[PspA] peaked rapidly, with the secondary response highly enriched for the 44.1-idiotype and lacking affinity maturation. These results demonstrate that noncovalent association in a particle, of polysaccharide and protein, recapitulates essential immunologic characteristics of intact bacteria that are distinct from soluble covalent conjugates of these respective Ags.
Identification of a Phage-Encoded Ig-Binding Protein from Invasive Neisseria meningitidis
Authors: Müller Maike G. , Ing Jessica Y. , Cheng Mike Kai-Wick , Flitter Becca A. , Moe Gregory R. 
Published: August 2013

Abstract
Ig-binding proteins are employed by a variety of organisms to evade the immune system. To our knowledge, we now report for the first time that meningococcal strains from several capsular groups exhibit Ig-binding activity that is dependent on human serum factors. A protein mediating Ig binding was identified as T and B cell–stimulating protein B (TspB) by immunoprecipitation and by mass spectroscopic analysis of tryptic peptides. Recombinant TspB and derivatives verified Ig binding, with a preference for human IgG2 Fc, and localized the IgG-binding region to a highly conserved subdomain of TspB. Antiserum produced in mice against the conserved subdomain detected the presence of TspB on the cell surface by flow cytometry when bacteria were grown in the presence of human serum. By fluorescence microscopy, we observed formation of an extracellular matrix having characteristics of a biofilm containing TspB, human IgG, DNA, and large aggregates of bacteria. TspB is encoded by gene ORF6 in prophage DNA, which others have shown is associated with invasive meningococcal strains. Knocking out ORF6 genes eliminated IgG binding and formation of large bacterial aggregates in biofilm. Reintroduction of a wild-type ORF6 gene by phage transduction restored the phenotype. The results show that TspB mediated IgG binding and aggregate/biofilm formation triggered by factors in human serum. As has been observed for other Ig-binding proteins, the activities mediated by TspB may provide protection against immune responses, which is in accordance with the association of prophage DNA carrying ORF6 with invasive meningococcal strains.
Crushed Muscle Tissue Graft: A Efficient Repair Technique for Intraoperative Chylous Fistulas
Authors: Gupta Sumit , Naik Sudhir , Halkud Rajshekar , Nanjundappa A , Shenoy Ashok M , Chavan Purshottam 
Published: August 2013

ABSTRACTBackground/objectivesChylous fistula is an uncommon but serious complication of radical neck dissections and is a complication which can be better avoided than treated. Presence of amber colored lymph in the postoperative closed wound suction drainage is defined as chylous fistula.Materials and methodsThe study was done over 18 months analyzing neck dissections done for 245 cases for complications of chyle leak. Six cases of chyle leak were identified during the course of the study.ResultsAll the six cases were repaired with crushed muscle tissue graft harvested from the remaining portion of the sternocleidomastoid muscle was spread out and sealed. No chyle leak complications were seen in the drains after the repair.ConclusionThe use of crushed local muscle tissue grafts offers better chances of success for closure of chylous fistulae in the neck, especially when the fistulous opening was difficult to ligate and the site of leak was only packed with sponge gel or Surgicel®. This complication should be better avoided than repaired.How to cite this articleNaik SM, Chavan P, Halkud R, Shenoy AM, Nanjundappa A, Gupta S. Crushed Muscle Tissue Graft: A Efficient Repair Technique for Intraoperative Chylous Fistulas. Int J Phonosurg Laryngol 2013;3(1):1-5.
Deep Neck Infections: Continuing Burden in Developing World
Authors: Kumar Surinder , Rathore Praveen Kumar , Rana Kanika 
Published: August 2013

ABSTRACTDeep neck infections (DNIs) continue to burden the health care system in developing countries like India. Aim of our study was to determine clinical features, diagnostic and therapeutic aspects of DNIs, various microorganisms involved in DNIs and their antibiotic sensitivity and to study the outcome of described treatment response. Fifty patients with DNIs were managed at the department of ENT in our hospital from September 2009 to September 2010. There were 29 male (58%) and 21 female (42%) patients with age ranging from 6 months to 75 years. Maximum patients presented in emergency department with complain of neck swelling and pain. Most common abscess encountered was of submandibular region. Odontogenic cause was found to be the most common cause of DNIs. Surgical intervention was required in 39 patients. No major complications were seen. It was observed that the low socioeconomic status along with illiteracy and lack of adequate management of early infection at primary health care level lead to prevalence of these infections in developing countries like ours.How to cite this articleRana K, Rathore PK, Wadhwa V, Kumar S. Deep Neck Infections: Continuing Burden in Developing World. Int J Phonosurg Laryngol 2013;3(1):6-9.
Stapler Laryngectomy
Authors: Varghese Bipin T 
Published: August 2013

ABSTRACTClosed stapling of the pharynx after careful separation of the laryngeal frame work from the adjacent pharynx is a viable option in total laryngectomy for endolaryngeal cancers. Author describes his experience and improvization that ensures success in stapler laryngectomy.How to cite this articleVarghese BT. Stapler Laryngectomy. Int J Phonosurg Laryngol 2013;3(1):10-11.
Angioleiomyoma of the Hypopharynx: A Rare Entity
Authors: Rangappa Vidya Bhushan , Suresh Raghavendra 
Published: August 2013

ABSTRACT
Leiomyomas are benign soft tissue neoplasms that arise from smooth muscle. Cases have been reported in the gastrointestinal tract, brain, oral cavity and other sites. Laryngeal angioleiomyomas are infrequently reported. Literature search revealed no case reported in the hypopharynx. We report a case of 40-year-old female with angioleiomyoma of the hypopharynx. External approaches have been described in the literature for laryngeal leiomyomas. We discuss the endolaryngeal approach for removal of these tumors.

How to cite this article
Suresh R, Balasubramanya AM, Rangappa VB. Angioleiomyoma of the Hypopharynx: A Rare Entity. Int J Phonosurg Laryngol 2013;3(1):28-30.
Fucoidan Cross‐Linking Polyacrylamide as Multifunctional Aqueous Binder Stabilizes LiCoO2 to 4.6 V
Authors: Cao Yulin , Cai Hongsheng , Xu Xin , Huang Yongcong , Zhang Fangchang , Liu Peiwen , Li Yingzhi , Nie Chenxi , Luo Wen , Mao Zhongzheng , Liao Chengzhu , Yang Mingyang , Luo Guangfu , Gu Shuai , Lu Zhouguang 
Published: July 2024

AbstractRaising the cutoff voltage can efficiently increase the energy density of lithium cobalt oxide (LCO). However, upon charging over 4.55 V the LCO undergoes irreversible phase transition from the pristine O3 phase to the metastable H1‐3 phases, causing serious side reactions, which results in poor cycling stability. Herein, a multifunctional aqueous composite binder derived from the cross‐linking of fucoidan (FUC) and polyacrylamide (PAM) is developed to enhance the stability of LCO cathode at 4.6 V. The cross‐linking interaction of FUC and PAM provides a uniform coating on the surface of LCO and ensures a high peel strength for the electrode, effectively mitigating irreversible phase transition and detrimental interface side reactions. More importantly, the sulfur ester and amide groups of FUC‐PAM favorably function as surface charge compensators to the high valent Co upon charging under high voltages, thus stabilizes the surface lattice of LCO and suppresses the detrimental oxygen release. As expected, the LCO with a cutoff voltage of 4.6 V exhibits a high capacity retention of 90% after 100 cycles at a current density of 110 mA g−1. The interfacial coordination effect of composite binders offers a novel strategy to enhance the stability of high‐voltage LCO for high‐energy lithium‐ion batteries.
In situ Ion Pumping Anodization to Confine Single‐Atom Fe onto TiO2 Nanotubes for Enhanced Noble‐Metal‐Free Photocatalytic Hydrogen Generation
Authors: Bi Jingtao , Li Chengqian , Ren Jie , Zhao Yingying , Ji Zhiyong , Wang Ting , Huang Xin , Hao Hongxun 
Published: July 2024

AbstractAchieving a high dispersity of deposited transition metal is crucial in the anodization preparation of noble‐metal‐free composites for photocatalytic hydrogen generation. Herein, for the first time, an in situ ion pumping anodization method is proposed to confine single‐atom Fe within the spatially ordered structure of anodized TiO2 nanotubes (TNT), assisted by ferric sodium salt of ethylenediaminetetraacetic acid (EDTA) as an “ion pump” to deliver Fe3+ toward anodes. Both experimental and theoretical calculations confirm that highly dispersed single‐atom Fe can interact with single‐electron‐trapped oxygen vacancies (SETOV), effectively reducing the oxygen vacancy concentration in TiO2 nanotubes. This reduction promotes efficient charge carrier separation through the Fe(III)/Fe(II) pathway and enhances hydrogen desorption, ultimately boosting the photocatalytic hydrogen evolution performance.
Poly(aryl piperidinium)‐Based AEMs Utilizing Spirobifluorene as a Branching Agent
Authors: Ryoo Geun Woong , Shin Sang‐Hun , Song In Wook , Lim Jiheon , Shin Hosun , Kwon Ki Chang , Park Sun Hwa , Lee Jang Yong , Kwon Min Sang 
Published: July 2024

AbstractThe high‐performance anion exchange membranes (AEMs) are developed by incorporating 9,9′‐spirobifluorene as a 3D branching agent, addressing the common trade‐off between ion conductivity and dimensional/mechanical stability. By fine‐tuning the ratio of terphenyl to biphenyl and the amount of the branching agent, the AEM is refined, achieving high conductivity (≈190 mS cm−1 at 80 °C in 1 m KOH) with decent dimensional/mechanical properties, comparable to the recently reported state‐of‐the‐art membranes. Investigations using gas pycnometer and atomic force microscopy demonstrated that spirobifluorene enhances the fractional free volume around the membrane's backbone and more precisely modulates the separation between hydrophobic and hydrophilic domains, thus boosting both ion conductivity and mechanical stability. This membrane also displayed excellent chemical stability, with negligible degradation at 80 °C in 1 m KOH over 1,000 h. With such a membrane, an excellent cell performance is achieved, with a current density of 11.2 A cm−2 at 80 °C and 2 V in 1 m KOH, and 1.8 A cm−2 at 80 °C and 2 V in pure water conditions. The in situ membrane stability test, conducted at a constant current density of 1 A cm−2 for 500 h, showed no significant degradation.
Ultrasensitive Wearable Pressure Sensors with Stress‐Concentrated Tip‐Array Design for Long‐Term Bimodal Identification
Authors: Xie Lingjie , Lei Hao , Liu Yina , Lu Bohan , Qin Xuan , Zhu Chengyi , Ji Haifeng , Gao Zhenqiu , Wang Yifan , Lv Yangyang , Zhao Chun , Mitrovic Ivona Z. , Sun Xuhui , Wen Zhen 
Published: July 2024

AbstractThe great challenges for existing wearable pressure sensors are the degradation of sensing performance and weak interfacial adhesion owing to the low mechanical transfer efficiency and interfacial differences at the skin–sensor interface. Here, an ultrasensitive wearable pressure sensor is reported by introducing a stress‐concentrated tip‐array design and self‐adhesive interface for improving the detection limit. A bipyramidal microstructure with various Young's moduli is designed to improve mechanical transfer efficiency from 72.6% to 98.4%. By increasing the difference in modulus, it also mechanically amplifies the sensitivity to 8.5 V kPa−1 with a detection limit of 0.14 Pa. The self‐adhesive hydrogel is developed to strengthen the sensor–skin interface, which allows stable signals for long‐term and real‐time monitoring. It enables generating high signal‐to‐noise ratios and multifeatures when wirelessly monitoring weak pulse signals and eye muscle movements. Finally, combined with a deep learning bimodal fused network, the accuracy of fatigued driving identification is significantly increased to 95.6%.
Optoelectronic Devices for In‐Sensor Computing
Authors: Ren Qinqi , Zhu Chaoyi , Ma Sijie , Wang Zhaoqing , Yan Jianmin , Wan Tianqing , Yan Weicheng , Chai Yang 
Published: July 2024

AbstractThe demand for accurate perception of the physical world leads to a dramatic increase in sensory nodes. However, the transmission of massive and unstructured sensory data from sensors to computing units poses great challenges in terms of power‐efficiency, transmission bandwidth, data storage, time latency, and security. To efficiently process massive sensory data, it is crucial to achieve data compression and structuring at the sensory terminals. In‐sensor computing integrates perception, memory, and processing functions within sensors, enabling sensory terminals to perform data compression and data structuring. Here, vision sensors are adopted as an example and discuss the functions of electronic, optical, and optoelectronic hardware for visual processing. Particularly, hardware implementations of optoelectronic devices for in‐sensor visual processing that can compress and structure multidimensional vision information are examined. The underlying resistive switching mechanisms of volatile/nonvolatile optoelectronic devices and their processing operations are explored. Finally, a perspective on the future development of optoelectronic devices for in‐sensor computing is provided.
Highly Biocompatible Graphite Electrodes by Using Interface‐Stable Coating and the Application to Hemodialysis
Authors: Xu Xinkai , Xu Yi , Guo Haitao , Zhou Zanxin , Hu Wenjie , Wang Leilei , Li Shuang , Wang Shugang , Zheng Xu , Gu Qi , Xia Yuan , Cui Jingqiang , Wang Guosheng , Su Yewang 
Published: July 2024

AbstractIn the treatment of kidney diseases such as chronic kidney disease (CKD) and acute tubular necrosis (ATN), prolonged contact between conductivity sensors and patients' bodily fluids is required, necessitating high biocompatibility for the electrodes. However, the widely used graphite electrodes exhibit limited biocompatibility, showing a cell survival rate of only 88% under indirect contact conditions, and <56% under direct contact conditions. Here, the surface detachment of graphite electrodes in liquid environments leading to cell death upon contact is observed and a solution is proposed to enhance biocompatibility and ensure conductivity, by forming a layer of interface‐stable coating (ISC) as a conductive isolation membrane on their surface. For applications with contact requirements, graphite‐like carbon (GLC) coated graphite electrodes are investigated and developed, resulting in an exceptional cell survival rate exceeding 96% under indirect contact conditions, and a relatively high survival rate exceeding 91% under direct contact conditions, both accompanied by significant proliferation. GLC‐coated graphite electrodes are successfully to monitor the dialysate conductivity in a hemodialysis machine and achieve stable monitoring with temperature compensation. The results demonstrate ISC graphite electrodes' potential in biomedical fluid monitoring, with the developed process applicable to other fields.
Fabrication of Flexible Double‐Gate Organic Thin Film Transistor For Tactile Applications
Authors: Concas Mattia , Mascia Antonello , Lai Stefano , Bonfiglio Annalisa , Cosseddu Piero 
Published: July 2024

AbstractIn this work, the development of a flexible Double‐Gate (DG) organic thin film transistor (DG‐OTFT), and its employment is reported for the realization of multimodal tactile sensors. Due to the self‐encapsulation of the stacked DG architecture, highly stable organic transistors are obtained that show almost negligible degradation after 6 months. Moreover, such configuration is also very useful for the development of sensing devices. In the case, one of the two gates is used to bias and set the working point of the devices, whereas the second one is connected to a polyvinylidene fluoride(PVDF)‐capacitor, a pyro/piezoelectric material. It is demonstrated that the charge displacement induced by the PVDF capacitor due to an applied external pressure or due to a temperature variation led to a reproducible variation of the device's output current. Using this approach high‐performing multimodal tactile sensors are obtained with sensitivity to up to 241 nA N−1 and 442 nA °C−1 respectively.
A New Perspective on the Homogeneous Coordinate System for Calculating Interatomic Distances and Their Derivatives in Terms of Internal Coordinates
Authors: Camargo Jesus , Lavor Carlile 
Published: July 2024

AbstractIn many chemical physics calculations, efficient computation of interatomic distances and their derivatives using internal coordinates is essential for understanding molecular structure and dynamics. This paper explores the homogeneous coordinate system as an alternative framework to simplify these calculations. It is proven that computing interatomic distances using the Euclidean model of 3D space requires almost 60% more operations than the homogeneous model, which may represent a significant difference in molecular dynamics simulations.