bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro cancer cell line luminescence‐based validation of anticancer phytocompounds obtained from Leucas biflora against HELA cervical and A549 lung cancer cells
Authors: Chitra Kandasamy , Sureshkumar Muthusamy , Muraleedharan Aiswarya , Selvamaleeswaran Ponnusamy , Selvankumar Thangaswamy , Thirumalaisamy Rathinavel , Alyami Nouf M. , Alharbi Sulaiman Ali 
Published: August 2024

AbstractCurrent research aims to screen the anticancer prospective of Leucas biflora phytocompounds against apoptotic regulator target protein essential for cancer progression. In gas chromatography–mass spectrometry analysis major phytocompounds such as tetracosahexaene, squalene, phytol, 22‐stigmasten‐3‐one, stigmasterol, fluorene, and 1,4‐dihydro were identified in ethanolic leaf extract of Leucas biflora. In vitro, the free radical scavenging potential of ethanolic leaf extract of Leucas biflora was examined through its DPPH and ABTS radical scavenging potential IC50 value 15.35 and 13.20 μg/ml, respectively. Dose‐dependent cytotoxicity was monitored against both A549 lung cancer and HELA cervical cancer cells. Leucas biflora ethanolic leaf extract highly reduces the cell viability of both HELA and A549 cells in in vitro cytotoxicity assays. Leucas biflora ethanolic extract produces 23.76% and 29.76% viability rates against A549 lung and HELA cervical cancer cell lines, and their IC50 values differ slightly at 95.80 and 90.40 μg/ml, respectively. In molecular docking analysis lung cancer target protein–ligand complex 5Y9T‐16132746 showed a maximum score of −14 kcal/mol by exhibiting stable binding affinity and interactions among all screened complexes. Based on docking score nine phytocompounds from Leucas biflora and two reference standard drugs were chosen for further analysis. Further validation reveals that the fluorene, 1,4‐dihydro possess good ADMET, Bioactivity and density functional theory indices.
Do auditory brainstem implants favor the development of sensory integration and cognitive functions?
Authors: BAŞ Banu , Gökay Nuriye Yıldırım , Aydoğan Zehra , Yücel Esra 
Published: August 2024

AbstractBackgroundInformation about the development of cognitive skills and the effect of sensory integration in children using auditory brainstem implants (ABIs) is still limited.ObjectiveThis study primarily aims to investigate the relationship between sensory processing skills and attention and memory abilities in children with ABI, and secondarily aims to examine the effects of implant duration on sensory processing and cognitive skills in these children.MethodsThe study included 25 children between the ages of 6 and 10 years (mean age: 14 girls and 11 boys) with inner ear and/or auditory nerve anomalies using auditory brainstem implants. Visual‐Aural Digit Span Test B, Marking Test, Dunn Sensory Profile Questionnaire were applied to all children.ResultsThe sensory processing skills of children are statistically significant and positive, and moderately related to their cognitive skills. As the duration of implant use increases, better attention and memory performances have been observed (p < .05).ConclusionThe study demonstrated the positive impact of sensory processing on the development of memory and attention skills in children with ABI. It will contribute to evaluating the effectiveness of attention, memory, and sensory integration skills, and aiding in the development of more effective educational strategies for these children.
Mesenchymal stem cells for osteoarthritis: Recent advances in related cell therapy
Authors: Lin Jianjing , Huang Jingtao , Jiao Zilu , Nian Mengyuan , Li Canfeng , Dai Yali , Jia Shicheng , Zhang Xintao 
Published: August 2024

AbstractOsteoarthritis (OA) is a degenerative joint disease that affects the entire joint and has been a huge burden on the health care system worldwide. Although traditional therapy and targeted cartilage cell therapy have made significant progress in the treatment of OA and cartilage regeneration, there are still many problems. Mesenchymal stem cells from various tissues are the most studied cell type and have been used in preclinical and clinical studies of OA, because they are more widely available, have a greater capacity for in vitro expansion, and have anti‐inflammatory and immunomodulatory properties compared to autologous chondrocytes. This article will systematically review the latest developments in these areas. It may provide new insights for improving OA and cartilage regeneration.
A comparison of 1‐ versus 3‐month regional anatomy exposure on learning outcomes of undergraduate medical students
Authors: Antipova Veronica , Siwetz Martin , Engelhardt Maren , Fellner Franz A. , Manhal Simone , Niedermair Julian F. , Ondruschka Benjamin , Pietras Sandra M. , Poilliot Amélie J. , Pretterklieber Michael L. , Wimmer‐Röll Monika , Wree Andreas , Hammer Niels 
Published: August 2024

AbstractRegional anatomy teaching forms a cornerstone of undergraduate medical education. Owing to an increase in teaching and learning content throughout the medical curriculum in recent years, contact hours and overall course durations in anatomy are under review worldwide. This study aimed to assess whether shortening the course content duration impacts learning gain and the ability to identify anatomical structures correctly. Undergraduate medical students of the Johannes Kepler University Linz (JKU;n = 310) and at the Medical University of Graz (MUG;n = 156) participating in regional anatomy courses were included. Whole body regional anatomy courses, including hands‐on dissection and accompanying lectures, were delivered over one or three months. Course content and examination mode were kept consistent, while the duration of knowledge delivery was one or three months, respectively. Objective structured practical examinations (OSPE) were then carried out on prosections for the neck, thorax, and abdomen. 3‐month course exposure resulted in significantly higher OSPE scores for the neck (49 vs. 37%), thorax (65 vs. 54%), and abdomen (65 vs. 45%), respectively. Further evaluation of the utility of different embalming types yielded higher 3‐month scores in the neck and thorax regions with Thiel‐embalmed tissues and thorax and abdomen regions in ethanol‐glycerin‐embalmed tissues. Course exposure over a more extended period, like three months, appears to be highly beneficial.
Prognostic impact of bridge or neoadjuvant induction chemotherapy in patients with resected oral cavity cancer: A nationwide cohort study
Authors: Hsu Cheng‐Lung , Wen Yu‐Wen , Wang Hung‐Ming , Hsieh Chia‐Hsun , Liao Chi‐Ting , Lee Li‐Yu , Ng Shu‐Hang , Lin Chien‐Yu , Chen Wen‐Cheng , Lin Jin‐Ching , Tsai Yao‐Te , Lee Shu‐Ru , Chien Chih‐Yen , Hua Chun‐Hung , Wang Cheng Ping , Chen Tsung‐Ming , Terng Shyuang‐Der , Tsai Chi‐Ying , Fan Kang‐Hsing , Yeh Chih‐Hua , Lin Chih‐Hung , Tsao Chung‐Kan , Cheng Nai‐Ming , Fang Tuan‐Jen , Huang Shiang‐Fu , Kang Chung‐Jan , Lee Li‐Ang , Fang Ku‐Hao , Wang Yu‐Chien , Lin Wan‐Ni , Hsin Li‐Jen , Yen Tzu‐Chen , Liao Chun‐Ta 
Published: August 2024

AbstractBackgroundWhile surgery remains the primary treatment for oral squamous cell carcinoma (OCSCC), induction chemotherapy (IC) can be used as a bridging or neoadjuvant therapy. This nationwide study in Taiwan examines the survival outcomes of OCSCC patients who received IC before surgery.MethodsWe analyzed data from 29,891 patients with OCSCC. Of these, 29,058 initially underwent surgery (OP group), whereas 833 received IC before surgery (IC + OP group). A propensity score (PS)‐matched analysis (4, 1 ratio, 3260 vs. 815 patients) was performed considering tumor subsite, sex, age, Charlson comorbidity index, clinical T1–T4b tumors, clinical N0–3 disease, and clinical stage I–IV.ResultsIn the PS‐matched cohort, the 5‐year disease‐specific survival (DSS) and overall survival (OS) rates were 65% and 57%, respectively. When comparing the OP and IC + OP groups, the 5‐year DSS rates were 66% and 62%, respectively (p = 0.1162). Additionally, the 5‐year OS rates were 57% and 56%, respectively (p = 0.9917). No significant intergroup differences in survival were observed for specific subgroups with cT4a tumors, cT4b tumors, cN3 disease, pT4b tumors, and pN3 disease. However, for patients with pT4a tumors, the OP group demonstrated superior 5‐year outcomes compared to the IC + OP group, with a DSS of 62% versus 52% (p = 0.0006) and an OS of 53% versus 44% (p = 0.0060). Notably, patients with cT2–3, cN1, and c‐Stage II disease in the IC + OP group were significantly more likely to achieve pT0–1 status (p < 0.05).ConclusionsFollowing PS matching, the IC + OP group generally exhibited similar prognosis to the OP group. However, for pT4a tumors, the OP group showed superior 5‐year outcomes. While IC may not universally improve survival, it could be advantageous for patients who respond positively to the treatment.
Antioxidant and Antibacterial Properties of Ethanolic Pot‐Pollen Extracts of Melipona beecheii and Determination of the Major Components by GC‐MS
Authors: Calderón‐Martínez Patricia , Yam‐Puc Alejandro , Ramón‐Sierra Jesús , Hernández‐Bolio Gloria , Hernández‐Núñez Emanuel , Zamora‐Bustillos Roberto , Ortiz‐Vázquez Elizabeth 
Published: August 2024

AbstractMelipona beecheii pot‐pollen is a natural product that has barely been studied, unlike other hive products such as honey and propolis. Its application has been reported since ancient times in traditional Mayan medicine, and it is also a functional food with high nutritional value. In the present study, samples of ethanolic pot‐pollen extracts from five locations in the Yucatán Peninsula were analyzed to determine their antibacterial and antioxidant properties. All the extracts showed activity against five medically important bacteria; Pseudomonas aeruginosa and Listeria monocytogenes were the most susceptible bacteria in all samples. The evaluated antioxidant activity was higher than that reported by other studies. Palmitic, linoleic, and linolenic fatty acids and their respective ethyl ethers were detected by Gas Chromatography‐Mass Spectrometry (GC‐MS) in all samples in different concentrations. Based on these results, pot‐pollen extract from Mama, Yucatán exhibited the best biological activities (Minimum Inhibitory Concentrations (MICs) between 6 and 40 mg/mL, EC50 DPPH 28 μg/mL, EC50 RP 30 μg/mL), which could be related to a higher content of unsaturated fatty acids and their ethyl esters. The present study demonstrates that M. beecheii pot‐pollen has therapeutic potential in addition to its benefits as a nutritional supplement.
Prenylated Flavanone Enantiomers with α‐Glucosidase Inhibitory Activity from the Root Bark of Morus alba
Authors: Bi Yan‐Xue , Tian Lin‐Lin , Bao Jie , Zhang Hua 
Published: August 2024

AbstractA focused chemical investigation into the polar fractions of a well‐known traditional Chinese medicine called Sang‐Bai‐Pi (the root bark of Morus alba) yielded a panel of prenylated flavanones. The new compounds were identified as four pairs of enantiomers (1a/1b–4a/4b) featuring the same constitution structure, on the basis of HRMS, NMR and ECD analyses. Several previously reported known racemic co‐metabolites were also analyzed and separated by HPLC on chiral columns, and the absolute configurations of pure enantiomers were established via ECD technique for the first time. The inhibition of these isolates against the antidiabetic target α‐glycosidase was further tested, with most of them showing decent inhibitory activity compared with the positive control acarbose. The interaction mechanism of two selected compounds (3a & 4b) was explored by kinetics experiment, which revealed a mixed type of inhibition pattern toward the enzyme.
In Vivo Delivery Processes and Development Strategies of Lipid Nanoparticles
Authors: Cheng Jiashun , Jian Lina , Chen Zhaolin , Li Zhuoyuan , Yu Yaobang , Wu Yihang 
Published: August 2024

AbstractLipid nanoparticles (LNPs) represent an advanced and highly efficient delivery system for RNA molecules, demonstrating exceptional biocompatibility and remarkable delivery efficiency. This is evidenced by the clinical authorization of three LNP formulations: Patisiran, BNT162b2, and mRNA‐1273. To further maximize the efficacy of RNA‐based therapy, it is imperative to develop more potent LNP delivery systems that can effectively protect inherently unstable and negatively charged RNA molecules from degradation by nucleases, while facilitating their cellular uptake into target cells. Therefore, this review presents feasible strategies commonly employed for the development of efficient LNP delivery systems. The strategies encompass combinatorial chemistry for large‐scale synthesis of ionizable lipids, rational design strategy of ionizable lipids, functional molecules‐derived lipid molecules, the optimization of LNP formulations, and the adjustment of particle size and charge property of LNPs. Prior to introducing these developing strategies, in vivo delivery processes of LNPs, a crucial determinant influencing the clinical translation of LNP formulations, is described to better understand how to develop LNP delivery systems.
Percutaneous transcatheter treatment for partial anomalous pulmonary right venous connection to the superior caval vein without atrial septal defect
Authors: Amici Andrea , Ewert Peter , Elek Miroslav , Georgiev Stanimir 
Published: August 2024

AbstractWe developed technique similar to transcatheter treatment for superior sinus venosus defects to treat a patient with a Partial Anomalous Pulmonary Venous Connection of the right upper pulmonary veins (RUPV) without an atrial septal defect. A double transseptal puncture was performed, and the left atrium (LA) was connected with the RUPV using a covered stent. The blood flow from the superior vena cava was directed to the right atrium (RA) using a second covered stent.
Successful transcatheter aortic valve replacement in a failing HAART 300 aortic valve annuloplasty ring: A case report
Authors: Abdel‐Razek Omar , El Khoury Alessandro , McGrail Daniel , Laham Roger J. 
Published: August 2024

AbstractTranscutaneous aortic valve replacement (TAVR) has evolved from a complex procedure meant only for patients at prohibitive risk for surgery to a commonly performed procedure across a wide variety of clinical scenarios including the treatment of failed aortic valve bioprosthesis. Annuloplasty rings in the aortic position such as HAART 300 (Biostable Science and Engineering) have been introduced in the management of native aortic regurgitation. Percutaneous management of failed bioprosthesis rings in the aortic position has not been widely described. We present a case of a 69‐year‐old man with recurrent aortic regurgitation successfully treated with TAVR using a SAPIEN 3 valve within a HAART 300 ring.
X‐linked ichthyosis presenting with cryptorchidism for orchidopexy: A rare anesthetic encounter and case report
Authors: Bhatta Sunil , Pandit Sukriti , Chaudhary Pratik , Chhetri Naresh Thapa 
Published: August 2024

Key Clinical MessageCutaneous scaling and associated clinical syndrome displayed in X‐linked ichthyosis mandates multidisciplinary care. Patient with ichthyosis confronts a numerous challenge to an anesthesiologist and demands a rigorous management. As these patients are very vulnerable perioperatively, meticulous care and support are utmost.AbstractIchthyosis is a group of genetic conditions distinguished by the appearance of hyperkeratotic scales on the skin's surface. X‐linked ichthyosis results from a mutation in the steroid sulfatase (STS) gene, which encodes the steroid sulfatase enzyme. Here we report a case of a 6‐year‐old child with X‐linked ichthyosis. He presented to our operation theater for correction of left‐sided undescended testis and underwent surgery uneventfully. To handle X‐linked ichthyosis perioperatively, meticulous planning and efficient anesthetic administration are critical.
Core myopathy in two siblings with a biallelic variant in the CACNA1S gene—A case series study
Authors: Khoeini Tara , Kariminejad Ariana , Nilipour Yalda , Ariaei Armin , Najmabadi Hossein , Arabshahi Mojtaba , Faraji Zonooz Mehrshid , Haghi Ashtiani Bahram 
Published: August 2024

Key Clinical MessageHomozygous variants of Calcium Voltage‐Gated Channel Subunit Alpha1 S (CACNA1S) gene mutation were previously identified as causes of periodic paralysis and congenital early‐onset myopathy, while it could be manifested as a late‐onset congenital core myopathy.AbstractCalcium Voltage‐Gated Channel Subunit Alpha1 S (CACNA1S) gene mutation has been linked to various neuromuscular conditions in recent years. Congenital myopathy with core‐like features is one of the cardinal associations reported previously, causing severe respiratory insufficiency and death in neonates. Informed consent was received from the patients. Subsequently, peripheral blood leukocytes were utilized to extract genomic DNA. Moreover, exome enrichment was implemented through the Twist Human Core Exome Kit (Twist Bioscience) and exome sequenced using Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA). Sanger sequencing using BIG Dye Terminators confirmed the presence of the final variant. Finally, the candidate variants were classified based on the American College of Medical Genetics and Genomics (ACMG) guidelines. In this report, we describe two siblings, who presented with childhood and late‐onset progressive muscle weakness, and had a homozygous variant in exon 2 of the CACNA1S gene defined as c.188C > A (p.Ala63Asp) (NM_000069.3). The SIFT, Polyphen2, CADD PHRED, and Mutation Taster analysis tools classified the variant as pathogenic/damaging. The muscle biopsy of the younger brother revealed intermyofibrillar network pattern disruption as cytoplasmic core‐like lesions. The muscle magnetic resonance imaging (MRI) reported grade IIa and IIb fatty changes. Finally, the electromyography (EMG) findings suggested a myopathic change pattern. This report illustrates the clinical variability in CACNA1S‐related myopathy by reviewing prior reports and adding newly found aspects, additionally expanding the gene defects associated with core myopathy.
Antiphospholipid syndrome with major arterial thrombosis, presenting as pulmonary thromboembolism, cerebrovascular accident, and coronary artery disease: A case report and literature review
Authors: Shirani Aria , Daraei Morteza , Shirani Aryan 
Published: August 2024

Key Clinical MessageAntiphospholipid syndrome is an immunopathologic disorder that should be considered in all patients with recurrent and/or unexplained thromboembolic events. Antiphospholipid antibodies are diagnostic markers, and anticoagulation therapy is the therapeutic and preventive strategy. Long‐term anticoagulation therapy is necessary, with careful attention to potential bleeding complications.
Pauci‐immune necrotizing glomerulonephritis in a 24‐year‐old female with negative ANCA antibodies: A rare case report
Authors: Hamsho Suaad , Dumirieh Sumaya , Sleiay Mouhammed , AlBaroudi Douha , Alshekh Muhamad Ali , Alahmad Marwa 
Published: August 2024

Key Clinical MessagePauci‐immune necrotizing glomerulonephritis (PING) is a small vessel renal vasculitis usually associated with the presence of antineutrophil cytoplasmic antibodies (ANCA) against myeloperoxidase or proteinase. A small proportion of PING patients do not have ANCA antibodies.AbstractA condition known as Pauci‐immune necrotizing glomerulonephritis, or PING for short, is a type of kidney inflammation that affects small blood vessels. This condition is typically linked with the existence of certain antibodies, specifically antineutrophil cytoplasmic antibodies or ANCA, which target myeloperoxidase or proteinase. However, it's worth noting that a minor percentage of individuals diagnosed with PING do not possess these ANCA antibodies. A 24‐year‐old woman with no previous medical history arrived at the ER due to various symptoms including joint pain, fever, difficulty swallowing, and shortness of breath. Despite multiple symptoms suggesting systemic lupus erythematosus (SLE), this diagnosis was ruled out based on the EULAR/ACR 2019 classification criteria and laboratory tests. Other potential diagnoses such as rheumatoid arthritis (RA) and eosinophilic garnulomatosis with polyaniitis (EGPA) were also excluded based on respective criteria. The patient was treated with a 3‐day course of methylprednisolone, followed by prednisolone, which improved her creatinine levels. Subsequent tests for P‐ANCA and C‐ANCA were negative. A kidney biopsy confirmed necrotizing glomerulonephritis, consistent with pauci‐immune vasculitis. A bronchoscopy revealed bleeding and hemorrhage in her lungs, but bacterial culture analysis was negative. The patient was given piperacillin, tazobactam, and vancomycin for septic coverage, as well as intravenous immunoglobulin (IVIg), which led to symptom improvement.
Multidisciplinary management of implant failure: A case report
Authors: Samadi Alaa , Ismaili Zouheir , El Yamani Amal 
Published: August 2024

Key Clinical MessageThis case report presents the interest of multidisciplinary management of extreme peri‐implantitis requiring removal of implant emphasizing the different surgical and showing that the ovate pontic of conventional bridge is an optimal alternative for rehabilitation of the premolar sector, despite the fact that its main diffusion has been in the anterior sector due to the high demand aesthetic.AbstractPeri‐implantitis leads to gradual peri‐implant bone loss. Severe and extreme cases lead to complete implant failure and imply lost implants have to be removed. Residual ridge deformity management after implant removal is one of the factors contributing to improved aesthetic and functional results. Various grafting procedures have been developed to achieve this goal. This report describes a case of implant removal from the upper right first premolar due to advanced peri‐implantitis in a 51‐year‐old female patient. Guided bone regeneration with a alloplastic bone graft and resorbable collagen membrane combined with roll pedicle connective tissue graftt was used for both socket and soft‐tissue augmentation. This was combined with ovate design conventional provisional bridge. After a 6‐month of healing phase, a perfect adaptation of the marginal gingiva around the provisional restoration was obtained. Cone beam computed tomography revealed significant bone fill and buccolingual dimensional stability. A conventional all‐ceramic bridge with an oval pontic design was chosen as a definitive prosthetic solution to compensate for edentulism and maintain the good aesthetic results. According to the encouraging result obtained in this clinical case, the conventional prosthetic restoration associated with surgical reconstruction of failing tissues can be considered as a successful treatment in the case of advanced peri‐implantitis requiring implant removal.
Direct Synthesis of Pd2+‐Rich Palladene Aerogels as Bifunctional Electrocatalysts for Formic Acid Oxidation Reaction and Oxygen Reduction Reaction
Authors: Wang Cui , Wei Wei , Georgi Maximilian , Hübner René , Steinbach Christine , Bräuniger Yannik , Schwarz Simona , Kaskel Stefan , Eychmüller Alexander 
Published: August 2024

AbstractIn this work, we developed a direct strategy to fabricate Palladene (i. e. Palladium metallene) aerogels and propose a temperature‐dependent growth mechanism. Besides the typical three‐dimensional networks and wrinkled surface morphologies, the as‐prepared
aerogel is endowed with abundant Pd2+. The as‐prepared
aerogel exhibits an excellent mass activity in the formic acid oxidation reaction and a good half‐wave potential in the oxygen reduction reaction in comparison with Pd/C and a Pd aerogel. This work expands the range of metal aerogels from the perspective of the building block units and demonstrates a direct approach to fabricate highly promising bifunctional electrocatalysts for fuel cells.