bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hollow fiber bioreactor as a stroma‐supported, serum‐free ex vivo expansion platform for human umbilical cord blood cells
Authors: Xue Cao , Kwek Kenneth Y. C. , Chan Jerry K. Y. , Chen Qingfeng , Lim Mayasari 
Published: January 2014

AbstractThe bone marrow microenvironment plays an integral role in the regulation of hematopoiesis. Residing stromal cells and the extracellular matrix in the bone marrow microenvironment provide biological signals that control hematopoietic stem cell (HSC) function. In this study, we developed a bio‐mimetic co‐culture platform using the hollow fiber bioreactor (HFBR) for ex vivo expansion of HSCs. We evaluated the efficacy of such a platform in comparison to standard cultures performed on tissue culture polystyrene (TCP), using a human stromal cell line (HS‐5) as stromal support, co‐cultured with lineage‐depleted human cord blood cells in serum‐free medium supplemented with a cytokine cocktail. Our results showed that the performance of the HFBR in supporting total cell and CD34+ progenitor cell expansion was comparable to that of cultures on TCP. Cells harvested from the HFBR had a higher clonogenic ability. The performance of ex vivo‐expanded cells from the HFBR in hematopoietic reconstitution in humanized mice was comparable to that of the TCP control. Scanning electron microscopy revealed that stroma cell growth inside the HFBR created a three‐dimensional cell matrix architecture. These findings demonstrate the feasibility of utilizing the HFBR for creating a complex cell matrix architecture, which may provide good in vitro mimicry of the bone marrow, supporting large‐scale expansion of HSCs.
Reduced graphene oxide hydrogels and xerogels provide efficient platforms for immobilization and laccase production by Trametes pubescens
Authors: Rodriguez‐Couto Susana , Arzac Alejandro , Leal Gracia Patricia , Tomovska Radmila 
Published: January 2014

AbstractFungal immobilization is an interesting topic in enzyme production and bioprocess development. The properties of graphene (i.e. large surface area, hydrophobicity), together with the possibility of producing it at low cost and with tailor‐made properties, make this popular material worthy of investigation as a support for fungal immobilization. In the present paper, 3D‐organized structures of reduced graphene oxide (rGO) in hydrogels and their dried derivatives (xerogels) were synthesized, characterized and investigated as potential supports for the immobilization of the white‐rot fungus Trametes pubescens. It was found that the morphology of the hydrogels and xerogels was not influenced by the synthesis conditions; however the 3D structure was preserved after drying and formation of xerogels. Both, hydrogels and xerogels have been shown to be suitable supports for the immobilization of T. pubescens. Additionally, xerogels promoted increased laccase activities and maximum activity values of about 20 ± 1 U/mL were attained. These activities were much higher than those obtained with other well‐known inert supports. Nevertheless, no relationship between support morphology and productivity was found. The encouraging results obtained have paved the way for the development of novel graphene‐based supports for microorganism immobilization.
Purification of chimeric heavy chain monoclonal antibody EG2‐hFc using hydrophobic interaction membrane chromatography: An alternative to protein‐A affinity chromatography
Authors: Sadavarte Rahul , Spearman Maureen , Okun Natalie , Butler Michael , Ghosh Raja 
Published: January 2014

ABSTRACTHeavy chain monoclonal antibodies are being considered as alternative to whole‐IgG monoclonal antibodies for certain niche applications. Protein‐A chromatography which is widely used for purifying IgG monoclonal antibodies is also used for purifying heavy chain monoclonal antibodies as these molecules possess fully functional Fc regions. However, the acidic conditions used to elute bound antibody may sometimes also leach protein‐A, which is immunotoxic. Low pH conditions also tend to make the mAb molecules unstable and prone to aggregation. Moreover, protein‐A affinity chromatography does not remove aggregates already present in the feed. Hydrophobic interaction membrane chromatography (or HIMC) has already been studied as an alternative to protein‐A chromatography for purifying whole‐IgG monoclonal antibodies. This paper describes the use of HIMC for capturing a humanized chimeric heavy chain monoclonal antibody (EG2‐hFC). Binding and eluting conditions were suitably optimized using pure EG2‐hFC. Based on this, an HIMC method was developed for capture of EG2‐hFC directly from cell culture supernatant. The EG2‐hFc purity obtained in this single‐step process was high. The glycan profiles of protein‐A and HIMC purified monoclonal antibody samples were similar, clearly demonstrating that both techniques captured similarly glycosylated population of EG2‐hFc. Moreover, this technique was able to resolve aggregates from monomeric form of the EG2‐hFc. Biotechnol. Bioeng. 2014;111: 1139–1149. © 2014 Wiley Periodicals, Inc.
Involvement of ERK and p38 MAPK pathways on Interleukin‐33‐induced RANKL expression in osteoblastic cells
Authors: Mine Yuichi , Makihira Seicho , Yamaguchi Yu , Tanaka Hideki , Nikawa Hiroki 
Published: January 2014

AbstractThe receptor activator of NF‐κB ligand (RANKL)/osteoprotegerin (OPG) system is a well‐known key factor in osteoclast differentiation, and osteoblastic lineage cells are the major sources of RANKL and OPG in local bone tissue. Recently, a new molecule from the interleukin (IL)‐1 family, IL‐33, was identified. Here, we report the possible involvement of IL‐33 in RANKL and OPG expression, and the signaling pathways that are required for maximal IL‐33‐induced RANKL expression in MC3T3‐E1 osteoblastic cells. Stimulation with IL‐33 increased the mRNA expression and secretion of RANKL in MC3T3‐E1 cells. The IL‐33‐induced RANKL mRNA expression was inhibited by an anti‐IL‐33 monoclonal antibody. Furthermore, ERK and p38 MAPK inhibitors, but not a JNK inhibitor, suppressed IL‐33‐induced RANKL mRNA expression. On the other hand, IL‐33 had no effect on OPG mRNA expression and protein secretion. These results taken together suggest that IL‐33 stimulates RANKL expression through mechanisms dependent on the ERK and p38 MAPK pathways in MC3T3‐E1 cells.
Highly Efficient Asymmetric Simmons–Smith Cyclopropanation Promoted by Chiral Heteroorganic Aziridinyl Ligands
Authors: Rachwalski Michał , Kaczmarczyk Sylwia , Leśniak Stanisław , Kiełbasiński Piotr 
Published: January 2014

AbstractEnantiomerically pure heteroorganic catalysts, bearing a hydroxy moiety, a stereogenic sulfinyl group, and a chiral aziridine moiety, have proved highly efficient in the asymmetric Simmons–Smith cyclopropanation of allylic alcohols, which generates the desired products in high yields (up to 95 %) and with ee values up to 94 %. The effect of the stereogenic centers at the sulfinyl sulfur atom and in the aziridine moiety on the stereochemical course of the title reaction is discussed.
New Insights into the Nature of Observable Reaction Intermediates in Cytochrome P450 NO Reductase by Using a Combination of Spectroscopy and Quantum Mechanics/Molecular Mechanics Calculations
Authors: Riplinger Christoph , Bill Eckhard , Daiber Andreas , Ullrich Volker , Shoun Hirofumi , Neese Frank 
Published: January 2014

AbstractCytochrome P450 NO reductase is an unusual member of the cytochrome P450 superfamily. It catalyzes the reduction of nitric oxide to nitrous oxide. The reaction intermediates were studied in detail by a combination of experimental and computational methods. They have been characterized experimentally by UV/Vis, EPR, Mössbauer, and MCD spectroscopy. In conjunction with quantum mechanics/molecular mechanics (QM/MM) calculations, we sought to characterize the resting state and the two detectable intermediates in detail and to elucidate the nature of the key intermediate I of the reaction. Six possible candidates were taken into account for the unknown key intermediate in the computational study, differing in protonation state and electronic structure. Two out of the six candidates could be identified as putative intermediates I with the help of the spectroscopic data: singlet diradicals FeIII‐NHO.− and FeIII‐NHOH.. In a companion publication (C. Riplinger, F. Neese, ChemPhysChem­ 2011, 12, 3192) we have used QM/MM models based on these structures and performed a kinetic simulation. The combination of these two studies shows the nature of the key intermediate to be the singlet diradical, FeIII‐NHOH..
Synthesis of Water‐Soluble Red‐Emitting Thienyl‐BODIPYs and Bovine Serum Albumin Labeling
Authors: Poirel Arnaud , Retailleau Pascal , De Nicola Antoinette , Ziessel Raymond 
Published: January 2014

AbstractInvited for the cover of this issue is the group of Raymond Ziessel at the University of Strasbourg. The image depicts the bright red fluorescence of a probe, both free in aqueous solution and when covalently linked to a model protein, such as BSA. Read the full text of the article at 10.1002/chem.201303988.
ChemInform Abstract: The Crystal Structure of GdZn3.
Authors: Bigun Inna , Kalychak Yaroslav M. 
Published: January 2014

AbstractGdZn3 is obtained in the attempt to prepare Gd30Zn65In5 and Gd25Zn70In5 by melting of the elements (corundum crucible, evacuated quartz tube, 1.
ChemInform Abstract: Encapsulated Monoclinic Sulfur for Stable Cycling of Li—S Rechargeable Batteries.
Authors: Moon San , Jung Young Hwa , Jung Wook Ki , Jung Dae Soo , Choi Jang Wook , Kim Do Kyung 
Published: January 2014

AbstractAn encapsulated sulfur electrode for Li—S rechargeable batteries consisting of well aligned monoclinic sulfur nanowires completely covered by a minimal amount of carbon shows excellent electrical performance.
ChemInform Abstract: Amino Oxazolines as a New Class of Organocatalyst for the Direct Intermolecular Asymmetric Aldol Reaction Between Acetone and Aromatic Aldehydes.
Authors: Saiyed Akeel S. , Bedekar Ashutosh V. 
Published: January 2014

AbstractThe synthesis of chiral amino oxazolines is described and the utility of the products is demonstrated in the asymmetric aldol reaction of acetone with reactive carbonyl compounds.
ChemInform Abstract: Copper‐Catalyzed Electrophilic Amination of Organolithiums Mediated by Recoverable Siloxane Transfer Agents.
Authors: Nguyen Minh H. , Smith Amos B. III 
Published: January 2014

AbstractThe use of the siloxane transfer agent enables the direct electrophilic amination of organolithium substrates under mild and simple conditions without the use of stoichiometric amounts of toxic heavy metals.
ChemInform Abstract: Synthesis of Aryl Ethers from Aromatic Carboxylic Acids.
Authors: Bhadra Sukalyan , Dzik Wojciech I. , Goossen Lukas J. 
Published: January 2014

AbstractTwo complementary protocols allow the decarboxylative etherification of aromatic carboxylate salts, and the alkoxy groups can be selectively installed in the ipso or in the ortho position.
ChemInform Abstract: Quinones Synthesis via Hydrogen Peroxide Oxidation of Dihydroxy Arenes Catalyzed by Homogeneous and Macroporous‐Polymer‐Supported Ruthenium Catalysts.
Authors: Abu‐Elfotoh Abdel‐Moneim , Tsuzuki Kazuyuki , Nguyen Tram Bao , Chanthamath Soda , Shibatomi Kazutaka , Iwasa Seiji 
Published: January 2014

AbstractThe polymer‐supported catalyst PSR furnishes similar yields but longer reaction times are required (not shown).
ChemInform Abstract: Imidazolium Perrhenate Ionic Liquids as Efficient Catalysts for the Selective Oxidation of Sulfides to Sulfones.
Authors: Zhang Bo , Li Su , Yue Shuang , Cokoja Mirza , Zhou Ming‐Dong , Zang Shu‐Liang , Kuehn Fritz E. 
Published: January 2014

AbstractThe utilization of a new perrhenate containing ionic liquid for the efficient, environmentally friendly oxidation of a broad range of aryl and alkyl sulfides to the corresponding sulfones is described.
ChemInform Abstract: CuI/1,10‐phen/PEG Promoted Decarboxylation of 2,3‐Diarylacrylic Acids: Synthesis of Stilbenes under Neutral and Microwave Conditions with an in situ Generated Recyclable Catalyst.
Authors: Zou Yong , Huang Qi , Huang Tong‐kun , Ni Qing‐chun , Zhang En‐sheng , Xu Tian‐long , Yuan Mu , Li Jun 
Published: January 2014

AbstractThe in situ generated [CuI(1,10‐phen)]2 complex, which can easily be recovered from the reaction mixture, reveals high catalytic activity and reusability.
ChemInform Abstract: Cobalt‐Catalyzed Annulation of Aryl Iodides with Alkynes.
Authors: Komeyama Kimihiro , Kashihara Tetsuya , Takaki Ken 
Published: January 2014

AbstractA cobalt‐catalyzed approach for the concise synthesis of naphthalenes by the annulation of aryl iodides with symmetrical alkynes is disclosed.
ChemInform Abstract: Metal‐Free Aziridination of Styrene Derivatives with Iminoiodinane Catalyzed by a Combination of Iodine and Ammonium Iodide.
Authors: Kiyokawa Kensuke , Kosaka Tomoki , Minakata Satoshi 
Published: January 2014

AbstractThis novel method can also be used for the aziridination of fullerenes whereas, only poor conversion is observed for aliphatic alkenes.
ChemInform Abstract: A One‐Pot Stereoselective Synthesis of Novel Polyfunctionalized Imidazolidines.
Authors: Olyaei Abolfazl , Karimi Mohsen Karbalaei , Razeghi Reza 
Published: January 2014

AbstractA facile one‐pot stereoselective synthesis of novel trans‐4,5‐dihydroxy‐imidazolidines is presented.
ChemInform Abstract: A One‐Pot Synthesis of 3‐Amino‐1H‐isoindol‐1‐one Thiazol‐2‐ylhydrazones.
Authors: Biitseva Angelina , Groth Ulrich , Hordiyenko Olga 
Published: January 2014

AbstractThe title compounds are obtained by reaction of phthalonitrile with thiosemicarbazide and α‐haloketones.
ChemInform Abstract: Substrate‐Promoted Ligand‐Free CuI Catalyzed S‐Arylation of 2‐Mercaptobenzothiazoles with Aryl Iodides in Water.
Authors: He Guozhen , Huang Yuan , Tong Yao , Zhang Jie , Zhao Dan , Zhou Shuangli , Han Shiqing 
Published: January 2014

AbstractAn efficient method for the cross‐coupling of 2‐mercaptobenzothiazoles with aryl iodides is developed.
ChemInform Abstract: Synthesis of 5‐Iodo‐1,2,3‐triazoles from Organic Azides and Terminal Alkynes: Ligand Acceleration Effect, Substrate Scope, and Mechanistic Insights.
Authors: Barsoum David N. , Brassard Christopher J. , Deeb Jason H. A. , Okashah Najeah , Sreenath Kesavapillai , Simmons J. Tyler , Zhu Lei 
Published: January 2014

AbstractThe improved method for the selective formation of 5‐iodo‐1,2,3‐triazoles proceeds via an iodoalkyne intermediate under mild conditions.
ChemInform Abstract: 1,3‐Dipolar Cycloadditions of the Versatile Intermediate Tetraethyl Vinylidenebisphosphonate.
Authors: Ferrer‐Casal Mariana , Barboza Alejandro P. , Szajnman Sergio H. , Rodriguez Juan B. 
Published: January 2014

AbstractThe use of the title phosphonate as dipolarophile in 1,3‐dipolar cycloadditions with azides, nitrile oxides, and azomethine ylides is described.
ChemInform Abstract: SnCl2/Nano SiO2: A Green and Reusable Heterogeneous Catalyst for the Synthesis of Polyfunctionalized 4H‐Pyrans
Authors: Safaei‐Ghomi Javad , Teymuri Raheleh , Shahbazi‐Alavi Hossein , Ziarati Abolfazl 
Published: January 2014

Abstractvia a one‐pot multicomponent cyclocondensation of aromatic aldehydes with CH acids, malononitrile and ethyl acetoacetate
ChemInform Abstract: Microwave Assisted Synthesis of New Coumarin Based 3‐Cyanopyridine Scaffolds Bearing Sulfonamide Group Having Antimicrobial Activity.
Authors: Desai N. C. , Satodiya H. M. , Rajpara K. M. , Joshi V. V. , Vaghani H. V. 
Published: January 2014

AbstractA variety of new chromenylpyridinyl sulfonamides (VI) (13 examples) are synthesized and screened for their antibacterial and antifungal activities.
ChemInform Abstract: Iron‐Catalyzed One‐Pot 2,3‐Diarylquinazolinone Formation from 2‐Nitrobenzamides and Alcohols.
Authors: Wang Huamin , Cao Xiangxiang , Xiao Fuhong , Liu Saiwen , Deng Guo‐Jun 
Published: January 2014

AbstractThis method is suitable for benzylic alcohol derivatives (II), but not for simple aliphatic alcohols (e.
ChemInform Abstract: Metallic Anodes for Next Generation Secondary Batteries
Authors: Kim Hansu , Jeong Goojin , Kim Young‐Ugk , Kim Jae‐Hun , Park Cheol‐Min , Sohn Hun‐Joon 
Published: January 2014

AbstractReview: focus on metallic Li anodes, but Mg, Na, Al, and Zn electrodes are included; 286 refs.
Recurrence of Left Ventricular Dysfunction in Patients With Restored Idiopathic Dilated Cardiomyopathy
Authors: Park Jin‐Sun , Kim Jin‐Woo , Seo Kyoung‐Woo , Choi Byoung‐Joo , Choi So‐Yeon , Yoon Myeong‐Ho , Hwang Gyo‐Seung , Tahk Seung‐Jea , Shin Joon‐Han 
Published: January 2014

AbstractBackgroundIn some patients with nonischemic idiopathic dilated cardiomyopathy (DCM), left ventricular (LV) dysfunction improves spontaneously but can recur. The factors predicting recurrence of LV dysfunction in recovered idiopathic DCM are poorly defined. We investigated the clinical, echocardiographic, and laboratory variables affecting recurrence of LV dysfunction in patients who recovered from DCM.HypothesisThe recurrence of LV dysfunction in recovered idiopathic DCM is impacted by clinical, echocardiographic, and laboratory variables.MethodsThe study comprised 85 consecutively enrolled patients (62 males, age 57 ± 16 years) with DCM who achieved a restoration of LV systolic function. Patients were followed up for 50 ± 33 months after recovery from LV dysfunction without discontinuation of standard medication for heart failure with depressed ejection fraction. Clinical, echocardiographic, and laboratory variables were analyzed to identify factors independently associated with recurrence of LV dysfunction.ResultsLV dysfunction recurred in 33 patients (23 males, age 64 ± 12 years). Univariate analysis revealed that age, duration from initial presentation to recovery time, diabetes, and LV end‐diastolic dimension (LVEDD) at initial presentation were associated with recurrence of LV dysfunction. Multivariate analysis revealed that only age, diabetes, and LVEDD at initial presentation were independent predictors in patients who recovered from LV dysfunction.ConclusionsThe recurrence of LV dysfunction was significantly correlated with age, presence of diabetes, and LVEDD at initial presentation. Clinicians should consider maintenance of intensive care to patients who recovered from DCM with these factors.
A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus
Authors: Gilland E. , Straka H. , Wong T.W. , Baker R. , Zottoli S.J. 
Published: January 2014

ABSTRACTThe vertebrate hindbrain develops as a series of well‐defined neuroepithelial segments or rhombomeres. While rhombomeres are visible in all vertebrate embryos, generally there is not any visible segmental anatomy in the brains of adults. Teleost fish are exceptional in retaining a rhombomeric pattern of reticulospinal neurons through embryonic, larval, and adult periods. We use this feature to map more precisely the segmental imprint in the reticular and motor basal hindbrain of adult goldfish. Analysis of serial sections cut in three planes and computer reconstructions of retrogradely labeled reticulospinal neurons yielded a segmental framework compatible with previous reports and more amenable to correlation with surrounding neuronal features. Cranial nerve motoneurons and octavolateral efferent neurons were aligned to the reticulospinal scaffold by mapping neurons immunopositive for choline acetyltransferase or retrogradely labeled from cranial nerve roots. The mapping corresponded well with the known ontogeny of these neurons and helps confirm the segmental territories defined by reticulospinal anatomy. Because both the reticulospinal and the motoneuronal segmental patterns persist in the hindbrain of adult goldfish, we hypothesize that a permanent “hindbrain framework” may be a general property that is retained in adult vertebrates. The establishment of a relationship between individual segments and neuronal phenotypes provides a convenient method for future studies that combine form, physiology, and function in adult vertebrates. J. Comp. Neurol. 522:2446–2464, 2014. © 2014 Wiley Periodicals, Inc.
Statistical analysis of Amenamevir (ASP2151) between pharmacokinetics and clinical efficacies with non‐linear effect model for the treatment of genital herpes
Authors: Takada Akitsugu , Katashima Masataka , Kaibara Atsunori , Sawamoto Taiji , Zhang Wenhui , Keirns James 
Published: January 2014

AbstractAmenamevir is the international non‐proprietary name for ASP2151 synthesized by Astellas Pharma, Inc. It is a structurally novel class of helicase‐primase inhibitor and demonstrated more potency in vitro anti‐viral activity with low cytotoxicity against varicella‐zoster virus (VZV), herpes simplex virus type 1 (HSV‐1), and herpes simplex virus type 2 (HSV‐2) than acyclovir (ACV). Phase II randomized trial assessed the safety and efficacy of ASP2151 for episodic therapy of recurrent genital herpes was conducted. Participants self‐initiated with ASP2151 (100, 200, or 400 mg daily for 3 days), ASP2151 (1,200 mg as a single dose), placebo for 3 days, or Valacyclovir (500 mg twice daily for 3 days). We present a first population pharmacokinetic (PPK) modeling analysis of Amenamevir for genital herpes patients. The final model retained the effect of Weight and Albumin on CL. Statistical analysis between pharmacokinetics and clinical efficacies was done by using the time above 200 ng/mL (T200). T200 derived from the final PPK model to consider the correlation with Time to lesion healing and viral shedding. This finding suggested that it could be necessary to maintain the Amenamevir concentration above the threshold level to prevent the virus replication.
Intensive removal of signal crayfish (Pacifastacus leniusculus) from rivers increases numbers and taxon richness of macroinvertebrate species
Authors: Moorhouse Tom P. , Poole Alison E. , Evans Laura C. , Bradley David C. , Macdonald David W. 
Published: January 2014

AbstractInvasive species are a major cause of species extinction in freshwater ecosystems, and crayfish species are particularly pervasive. The invasive American signal crayfish Pacifastacus leniusculus has impacts over a range of trophic levels, but particularly on benthic aquatic macroinvertebrates. Our study examined the effect on the macroinvertebrate community of removal trapping of signal crayfish from UK rivers. Crayfish were intensively trapped and removed from two tributaries of the River Thames to test the hypothesis that lowering signal crayfish densities would result in increases in macroinvertebrate numbers and taxon richness. We removed 6181 crayfish over four sessions, resulting in crayfish densities that decreased toward the center of the removal sections. Conversely in control sections (where crayfish were trapped and returned), crayfish density increased toward the center of the section. Macroinvertebrate numbers and taxon richness were inversely correlated with crayfish densities. Multivariate analysis of the abundance of each taxon yielded similar results and indicated that crayfish removals had positive impacts on macroinvertebrate numbers and taxon richness but did not alter the composition of the wider macroinvertebrate community. Synthesis and applications: Our results demonstrate that non‐eradication‐oriented crayfish removal programmes may lead to increases in the total number of macroinvertebrates living in the benthos. This represents the first evidence that removing signal crayfish from riparian systems, at intensities feasible during control attempts or commercial crayfishing, may be beneficial for a range of sympatric aquatic macroinvertebrates.