bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pertumbuhan dan Hasil Kedelai Edamame (Glycine max (L.) Merr.) pada Tanah Gambut yang diberi Dolomit dan Pupuk Hayati
Authors: Asie Erina Riak , Rumbang Nyahu , Asie Kambang Vetrani , Syahrudin Syahrudin , Darmawan Darmawan 
Published: August 2024

Edamame soybeans are soybeans that are harvested young so they are known as green soybeans. The high nutritional content and delicious taste make edamame soybeans popular with the public, so they have the potential to be developed. One effort to increase edamame production is through expanding the planting area (Extensification). One type of soil that has the potential for expanding edamame soybean plants is peat soil. This research aims to examine the effect of dolomite and biofertilizer on the growth and yield of edamame on peat soil. The experiment was carried out using a two-factor factorial Completely Randomized Design (CRD) with three replications. The first factor, namely dolomite (0, 5, 10, 15 t ha-1) and the second factor, namely biofertilizer (0, 5, 10, 15 g kg-1 seed). The research results showed that the interaction between dolomite and biofertilizer and the single factor of biofertilizer had no significant effect on the growth and yield of edamame soybeans. Theresults showed that the interaction between dolomite and biofertilizer and the single factor of biofertilizer had no significant effect on the growth and yield of edamame soybeans. The application of dolomite can increase leaf area, plant dry weight, number of pods, and fresh pod weight plant-1 of edamame soybean plants on peat soil.

Keywords: extensification, edamame, dolomite, biofertilizer, peat soil
Keragaan dan Karakter Fase Pertumbuhan In Vitro Putatif Mutan Anggrek Hitam Kalbar Hasil Induksi Mutasi Secara Kimia
Authors: Asnawati Asnawati , Listiawati Agustina 
Published: August 2024

Putative mutants of West Kalimantan black orchids obtained through mutation activities need to be evaluated for their growth character and performance early, starting from the vegetative phase at the in vitro stage. This study aims to determine the performance and character of the in vitro putative growth phase of West Kalimantan black orchid mutants. The research was carried out at the Biotechnology Laboratory of the Faculty of Agriculture, Tanjungpura University from April-October 2023. The experiment was conducted using Complete Randomized Design. As a treatment were 6 in vitro bud clones of black anggtek (5 putative mutant clones derived from 5 EMS concentration levels and 1 wild type plant clone as control). The clones are K (as control plant) and M, N, O, P and Q clones each resulting from EMS concentration 0; 0,25; 0,50; 0,75; 1.00 and 1.25%. The treatment was repeated 5 times and each repetition consisted of 5 samples, resulting in a total of 150 experimental units. Variables observed include: Changes in plant weight, time of emergence of shoots, time of emergence of roots, increase in the number of leaves, number of shoots and number of roots. The results showed that the increase in the number of leaves and weight of putative mutant plants endemic to West Kalimantan black orchids from clone M was better than control plants (K) and other clones (clones N, O, P and Q), For the character of the number of shoots and roots, all mutant putative clones produced a lower number of shoots and roots than control plants (K). The results of plant characterization, found differences in growth characters in all putative clones of black orchid mutants except the O clone, when compared to control plants.
Keywords : Black orchid, In vitro, Performance, Putatif Mutant, Vegetative growth
Faktor Yang Mempengaruhi Kualitas Hidup Pasien Penderita Diabetes Melitus Tipe II Di Puskesmas Sawah Lebar Kota Bengkulu
Authors: Zuzetta Tata , Pudiarifanti Nadia , Sayuti Noviandi 
Published: August 2024

Prevalensi penyakit diabetes di ndonesia terus meningkat. Berdasarkan hasil riskesdas tahun 2013 dan 2018, terjadi peningkatan sebesar 2,5% dari tahun ketahun. Selain itu, penyakit diabetes juga menjadi penyebab kematian tertinggi dan menempati urutan ke 3 di dunia. Diabetes melitus merupakan penyakit menahun dan bersifat seumur hidup sehingga mempengaruhi kualitas hidup penderitanya. Ada banyak faktor yang mempengaruhi kualitas hidup. Penelitan ini merupakan penelitian analitik observasional dengan desain penelitian cross sectional. Sampel dalam penelitian ini yaitu sebanyak 53 orang pasien DM di Puskesmas Sawah Lebar Kota Bengkulu. Teknik pengambilan sampel menggunakan teknik purposive sampling. Alat ukur yang digunakan pada penelitian ini yaitu kuisioner Sf- 36. Penelitian ini menggunakan analisis data kruskal wallis untuk mengetahui faktor yang mempengaruhi Kualitas Hidup Pasien Diabetes Melitus Tlipe II di Puskesmas Sawah Lebar Kota Bengkulu. Hasil penelitian yang didapatkan yaitu faktor yang mempengaruhi Kualitas Hidup Pasien Diabetes Melitus Tlipe II Di Puskesmas Sawah Lebar Kota Bengkulu meluputi usia (p value = 0,008), status pernikahan (p value = 0,024), lama menderita diabetes p value=0,015) dan lama penggunaan obat/ kepatuhan pengobatan (p value =0, 024). Sedangkan variabel yang tidak mempengaruhi terdiri dari jenis kelamin (p value=1,000), pendidikan (p value= 0,178), pekerjaan (p value = 0,688), terapi yang digunakan (p value= 0,082) dan penyakit penyerta (p value 0,185). Faktor yang mempengaruhi kualitas hidup pasien diabete melitus tipe II di Puskesmas Sawah Lebar Kota Bengkulu yaitu usia, status pernikahan, lama menderita dan lama penggunaan obat.
PENGARUH METODE PEMICUAN STBM TERHADAP PERUBAHAN PENGETAHUAN, SIKAP DAN TINDAKAN MASYARAKAT DALAM BABS
Authors: Zainal  , Indarwati Suami 
Published: August 2024

Abstrak : Pada Tahun 2015 dari 3.723 penduduk yang ada di Tanjung Agung, angka Buang Air Besar Sembarangan (BABS) sebesar 960 penduduk (25,78%). Pada tahun 2016 dari 3.757 penduduk yang ada, angka Buang Air Besar Sembarangan (BABS) sebesar 995 penduduk (26.48%) dan pada tahun 2017 dari 3.786 penduduk yang ada, angka Buang Air Besar Sembarangan (BABS) sebesar 1.017 penduduk (26.86%) yang terdiri dari 68 Kepala Keluarga. Penelitian ini bertujuan untuk mengetahui pengaruh metode pemicuan Sanitasi Total Berbasis Masyarakat (STBM) terhadap perubahan pengetahuan, sikap dan tindakan masyarakat dalam buang air besar sembarangan (BABS). Hasil penelitian Ada pengaruh pemicuan STBM terhadap pengetahuan,sikap dan tindakan masyarakat di Desa Tanjung Agung Kecamatan Teluk Pandan Kabupaten Pesawaran Pada Tahun 2019

Abstract: In 2015, out of 3,723 residents in Tanjung Agung, the number of open defecation was 960 residents (25.78%). In 2016, out of 3,757 people, the open defecation rate was 995 people (26.48%) and in 2017, out of 3,786 people, the open defecation rate was 1,017 people (26.86%) that was consisted of 68 heads of families. The aim of this research was to determine the effect of the community-based total sanitation triggering method on changes in knowledge, attitudes and community actions in open defecation. The results of this research founded that there was an effect of the triggering of open defecation rate on the knowledge, attitudes and actions of the community in Tanjung Agung Village, Teluk Pandan District, Pesawaran Regency in 2019
EXPLORATION OF BEHAVIOURAL MOTIVES IN CONSUMPTION OF 0% ALCOHOL DRINKS BY MUSLIM YOUTHS IN INDONESIA
Authors: Alam Azhar , Ratnasari Ririn Tri , Mawardi Imron , Amanda Sabilla Friska , Mellinia Rashifahunnisa' 
Published: August 2024

This study aims to discover the motives behind several Muslim youths in Indonesia who consume 0% alcohol drinks from popular alcohol brands even though the drink is not halal certified. This phenomenological research describes a factual reality based on the informant's experience as a consumer of 0% alcohol drink. Data sources were obtained through direct and indirect interviews with 15 informants, namely Muslim youth who consume 0% alcohol with an age range of 15-25 years. The results of this study indicate that there are internal and external motives behind Muslim youths consuming 0% alcohol. Internal motives from within Muslim adolescents include curiosity and the desire to try as an alternative to drinking, as well as to relieve stress and to refresh themselves. Meanwhile, external motives or influences from outside the Muslim youth include invitations from peers and environmental influences. This research also identified that some perpetrators who drink 0% of alcoholic drinks made by popular alcohol brands have low awareness of the halal label. This study contributes to global research by revealing how Muslim youth in Indonesia, driven by curiosity and peer influence, consume 0% alcohol drinks despite the lack of halal certification. This study suggests increasing halal awareness among Muslim adolescents through education and encouraging the Indonesian Ulema Council to emphasize the importance of halal certification, particularly for 0% alcohol drinks, to guide informed consumer choices.
Scientific and Systematic Approaches to Modeling of EFL Peer Learning in Higher Education
Authors: Makarova Elena 
Published: August 2024

The paper deals with EFL peer learning at a non-linguistic university. Lack of understanding of the mechanism of interactive learning as a system of relationships and interactions hinders creating the conditions for effective education. The aim of the study is to investigate theoretical and practical data on interactive learning, to design and describe the methodology for EFL peer learning in the form of educational models. The material includes basic postulates of constructivism, ecolinguistics, practice-oriented learning, peer learning and tutoring. Methods of modeling, experiment, observation and analysis are used. As a result, the structural components of interactive learning, their interconnections have been established and analyzed, the principles of interactive peer learning were formulated, including student activity; educational material selected in compliance with student’s knowledge; cyclical stages of a learning process; teacher’s preparatory work; classroom peer tutoring. Based on these principles, five models of peer learning have been designed each of which is centered around active interactions of students swapping the roles “tutor” - “tutee”. They are “Pair work”, “Group work”, “Peer assessment”, “Blended interactions”, “Extracurricular activities”. These models ensure maximum student involvement in EFL learning process, help increase motivation and level of language proficiency, develop students’ communication skills.
Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3
Authors: Wang Haojie , Chen Jianxing , An Tongqing , Chen Hongyan , Wang Yue , Zhu Liangquan , Yu Changqing , Xia Changyou , Zhang He 
Published: August 2024

IntroductionMuscovy duck parvovirus (MDPV), Goose parvovirus (GPV), Duck circovirus, (DuCV) and Duck adenovirus 3 (DAdV-3) are important pathogens that cause high morbidity and mortality in ducks, causing huge economic loss for the duck industry.MethodsThe present study, a quadruplex one-step real time quantitative PCR method for the detection of MDPV, GPV, DuCV, and DAdV-3 was developed. ResultsThe results showed that assay had no cross-reactivity with other poultry pathogens [Duck plague virus (DPV), Duck tembusu virus (DTMUV), H6 avian influenza virus (H6 AIV), New duck reovirus (NDRV), Newcastle disease virus (NDV), H4 avian influenza virus (H4 AIV), Escherichia coli (E. coli), Muscovy duck reovirus (MDRV), Egg drop syndrome virus (EDSV), Pasteurella multocida (P. multocida)]. The sensitivity result showed that the limits of detection for MDPV, GPV, DuCV, and DAdV-3 were 10, 10, 1 and 10 copies/µl, respectively; The coefficients of variation intra- and inter-method was 1-2%; The range of linear (109 to 103 copies/µL) demonstrated the R2 values for MDPV, GPV, DuCV, and DAdV-3 as 0.9975, 0.998, 0.9964, and 0.996, respectively. The quadruplex real time quantitative PCR method efficiency was 90.30%, 101.10%, 90.72%, and 90.57% for MDPV, GPV, DuCV, and DAdV-3, respectively. 396 clinical specimens collected in some duck sausages from June 2022 to July 2023 were simultaneously detected using the established quadruplex real time quantitative PCR method and the reported assays. The detection rates for MDPV, GPV, DuCV, and DAdV-3 were 8.33% (33/396), 17.93% (71/396), 33.58% (133/396), and 29.04% (115/396), respectively. The agreement between these assays was greater than 99.56%.DiscussionThe developed quadruplex real-time quantitative PCR assay can accurately detect these four viruses infecting ducks, providing a rapid, sensitive, specific and accurate technique for clinical testing.
The effects of exercise on microRNA expression profiling in adipose tissue macrophages of mice
Authors: Qin Fei , Huang Wenbai , Qu Chaoyi , Zhao Lina , Du Yunyu , Zhao Tianyu , Feng Yiwei , Zhao Jiexiu 
Published: August 2024

BackgroundExercise is recognized for its broad health benefits, influencing various physiological processes, including the behavior of adipose tissue macrophages (ATMs). While existing studies mainly associate ATM activity with obesity and metabolic syndrome, our study explores the impact of aerobic exercise on ATM microRNA expression profiling in a non-obese context, highlighting its general health-promoting mechanisms.MethodsSixty male C57BL/6 mice were randomly assigned to either a sedentary (S) or an exercise (E) group. The S group remained inactive, while the E group underwent a one-week treadmill adaptation, followed by an 8-week aerobic treadmill exercise protocol (60 min/day, 5 days/week, at 65%-75% VO2max). Post-training, glucose tolerance and the serum lipid levels were measured in mice subjected to both exercise and non-exercise conditions. ATMs harvested from visceral adipose tissues were analyzed and sorted using flow cytometer. To further investigate the effects of exercise in ATMs at the molecular level, miRNA microarray analysis was performed, followed by bioinformatic analysis.ResultsThe 8-week regimen of moderate-intensity aerobic exercise ameliorated glucolipid metabolism and fostered a dynamic shift toward an M2 macrophage phenotype in the adipose tissue, independent of obesity. A total of 62 differentially expressed miRNAs were identified in ATMs of mice post-exercise. Notably, six miRNAs (miR-212-5p, miR-511-5p, miR-7b-5p, miR-142-3p, miR-1894-3p, and miR-31-5p) as well as their target gene were consistently altered and associated with macrophage polarization and metabolic regulation.ConclusionOur findings broaden the understanding of how exercise regulates ATM functions through significant changes in microRNA profiles, emphasizing its potential to enhance health and prevent chronic conditions. This study supports the application of aerobic exercise for its preventive effects on chronic diseases and underscores the importance of microRNA profiling in understanding the immune-modulatory impacts of exercise.
Intensive oral prophylaxis does not alter the tongue microbiome in young patients with chronic kidney disease: longitudinal, randomized, controlled study
Authors: Hoefer Karolin C. , Weber Lutz T. , Barbe Anna Greta , Graf Isabelle , Thom Stefanie , Ehren Rasmus , Nowag Angela , Wisplinghoff Hilmar , Noack Michael J. , Scholz Claus J. , Jazmati Nathalie 
Published: August 2024

IntroductionGingivitis is a common intraoral disease in patients with chronic kidney disease (CKD), which poses a particular interdisciplinary challenge. We aimed to determine the influence of an intensive oral prophylaxis program (OPP) compared to standard prevention measures on the tongue microbiome of young patients with CKD.MethodsThirty patients with CKD (mean age 14.2 ± 5.2 years) and generalized gingivitis were included. The effects of the intensive OPP were compared with standard prophylaxis according to statutory health insurance (treatment as usual, TAU) as a control. Tongue swabs were taken from the patients at baseline (t1) and after 3 (t2) and 6 (t3) months. Next-generation sequencing of 16S rDNA genes was used to quantitatively characterize microbial communities.ResultsThere were no differences in the abundance, richness, or diversity of the observed genera and species between the two study groups at baseline or after 3 or 6 months. Furthermore, no change in predefined gingivitis and oral health bacterial clusters were found. At the phylum level, Firmicutes were decreased after intervention in the TAU group (t2TAU 42.9 ± 7.1 to t3TAU 34.8 ± 4.7 (npairs=14), p=0.003; false discovery rate 0.02). The decrease of Firmicutes was not significant in the OPP group.ConclusionsDespite the intensity of dental prophylaxis and decreasing clinical signs of inflammation and decreasing plaque amount, no clinically relevant changes in the tongue microbiome were observed. Our results confirm the conserved and stable nature of the tongue microbiome, even in children with CKD.
Heterophoria in multiple sclerosis patients: a proof of principle cross-sectional study
Authors: Graf Jonas , Weise Margit , Guthoff Tanja , Balloff Carolin , Gasis Marcia , Link Heike , Küchlin Sebastian , Lagrèze Wolf , Meuth Sven G. , Aktas Orhan , Albrecht Philipp 
Published: August 2024

ObjectivesThe pathophysiology of multiple sclerosis (MS) involves inflammatory neurodegeneration in the brainstem, cerebellum, and retina. The clinical relevance of oculomotor involvement in MS, however, remains uncertain.MethodsIn this cross-sectional study, we evaluated heterophoria as a (sub)clinical tool in 54 MS patients and 55 age-matched healthy controls (HCs). We quantified heterophoria in prism diopters for distance and near range with orthoptic examination. Our primary outcome was high degrees of horizontal heterophoria (HDHH) defined as measurements beyond ±2 standard deviations from the mean prism diopter of heterophoria of our HCs.ResultsMore than one-third (37%, n=20/54) of MS patients but only 11% (n=6/55) of HCs were classified as HDHH [distance, MS=9% (n=5/54) versus HC=6% (n=3/55); near, MS=19% (n=10/54) versus HC=5% (n=3/55)]. Our MS patients presented more combined vertical and horizontal deviations at near range [MS 19% (n=10/54) versus for HC 7% (n=4/55)]. We observed the combination of HDHH both at distance and at near testing in 9% (n=5/54) of MS patients but not at all in HCs (n=0/55).DiscussionDespite the high prevalence of heterophoria, HDHH may be an additional (sub)clinical tool of subclinical involvement in MS. Thus, orthoptic examination may be an additional tool to improve MS diagnostic procedures.
Do immunosuppressive treatments influence immune responses against adenovirus-based COVID-19 vaccines in patients with multiple sclerosis? An Argentine multicenter study
Authors: Silva Berenice Anabel , Miglietta Esteban , Casabona Juan Cruz , Wenker Shirley , Eizaguirre María Bárbara , Alonso Ricardo , Casas Magdalena , Lázaro Luciana Grimanesa , Man Federico , Portuondo Gustavo , Lopez Bisso Abril , Zavala Noelia , Casales Federico , Imhoff Gastón , Steinberg Dra Judith , López Pablo Adrián , Carnero Contentti Edgar , Deri Norma , Sinay Vladimiro , Hryb Javier , Chiganer Edson , Leguizamon Felisa , Tkachuk Verónica , Bauer Johana , Ferrandina Flavia , Giachello Susana , Henestroza Paula , Garcea Orlando , Pascuale Carla Antonela , Heitrich Mauro , Podhajcer Osvaldo L. , Vinzón Sabrina , D’Alotto-Moreno Tomas , Benatar Alejandro , Rabinovich Gabriel Adrián , Pitossi Fernando J. , Ferrari Carina C. 
Published: August 2024

IntroductionThere are no reports in LATAM related to longitudinal humoral and cellular response to adenovirus based COVID-19 vaccines in people with Multiple Sclerosis (pwMS) under different disease modifying therapies (DMTs) and neutralization of the Omicron and Wuhan variants of SARS-COV-2.MethodsIgG anti- SARS-COV-2 spike titer were measured in a cohort of 101 pwMS under fingolimod, dimethyl fumarate, cladribine and antiCD20, as well as 28 healthy controls (HC) were measured 6 weeks after vaccination with 2nd dose (Sputnik V or AZD1222) and 3nd dose (homologous or heterologous schedule). Neutralizing capacity was against Omicron (BA.1) and Wuhan (D614G) variants and pseudotyped particles and Cellular response were analyzed.ResultsMultivariate regression analysis showed anti-cd20 (β= -,349, 95% CI: -3655.6 - -369.01, p=0.017) and fingolimod (β=-,399, 95% CI: -3363.8 - -250.9, p=0.023) treatments as an independent factor associated with low antibody response (r2 adjusted=0.157). After the 2nd dose we found a correlation between total and neutralizing titers against D614G (rho=0.6; p&lt;0.001; slope 0.8, 95%CI:0.4-1.3), with no differences between DMTs. Neutralization capacity was lower for BA.1 (slope 0.3, 95%CI:0.1-0.4). After the 3rd dose, neutralization of BA.1 improved (slope: 0.9 95%CI:0.6-1.2), without differences between DMTs. A fraction of pwMS generated anti-Spike CD4+ and CD8+ T cell response. In contrast, pwMS under antiCD20 generated CD8+TNF+IL2+ response without differences with HC, even in the absence of humoral response. The 3rd dose significantly increased the neutralization against the Omicron, as observed in the immunocompetent population.DiscussionFindings regarding humoral and cellular response are consistent with previous reports.
Characterisation of reproductive tract microbiome and immune biomarkers for bovine genital campylobacteriosis in vaccinated and unvaccinated heifers
Authors: Juli Mst Sogra Banu , Raza Ali , Forutan Mehrnush , Siddle Hannah V. , Fordyce Geoffry , Muller Jarud , Boe-Hansen Gry B. , Tabor Ala E. 
Published: August 2024

BackgroundBovine genital campylobacteriosis (BGC) is a globally important venereal disease of cattle caused by Campylobacter fetus subspecies venerealis. Diagnosis of BGC is highly challenging due to the lack of accurate diagnostic tests.MethodsTo characterise the biomarkers for C. fetus venerealis infection, a total of twelve cycling heifers were selected and categorised as vaccinated (n = 6) with Vibrovax® (Zoetis™) and unvaccinated (n = 6). All heifers were oestrous synchronised with a double dose of prostaglandin (PGF2α) 11 days apart and when in oestrous intravaginally challenged with 2.7 x 109 CFU live C. fetus venerealis. DNA extracted from vaginal mucus samples was screened using a C. fetus qPCR and 16S rRNA was characterised using Illumina sequencing (V5-V8 region). Relative abundances of serum proteins were calculated using sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) for all heifers at three timepoints: pre-challenge, post-challenge and post-recovery.ResultsIn 16S rRNA sequencing of vaginal mucus, Campylobacter spp. appeared two days following challenge in unvaccinated compared to 14 days in vaccinated animals, consistent with the qPCR results. Increased relative abundances of Firmicutes and Campylobacterota were identified after C. fetus venerealis challenge and were associated with C. fetus venerealis in vaccinated and unvaccinated heifers. Greater relative abundance of Streptococcus spp. was observed during oestrous rather than dioestrous. In both vaccinated and unvaccinated heifers, Acinetobacter spp. increased after challenge with higher abundance of Corynebacterium spp. in the vaccinated group. A total of 130 unique proteins were identified in SWATH analysis of the serum samples, and the number of differentially abundant proteins found was higher in the vaccinated group after recovery from infection compared to pre-and post-challenge (adjusted P &lt; 0.05 and Log2FC &gt; 0.2).ConclusionCoglutinin, clusterin, HP homologs, vitamin D binding protein and fetuin B were identified as potential biomarkers for C. fetus venerealis infection and need further study to validate their efficiency as immune biomarkers for BGC.