bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo development
Authors: Zhang Chun-Hui , Qian Wei-Ping , Qi Shu-Tao , Ge Zhao-Jia , Min Ling-Jiang , Zhu Xiu-Lang , Huang Xin , Liu Jing-Ping , Ouyang Ying-Chun , Hou Yi , Schatten Heide , Sun Qing-Yuan 
Published: April 2013

Abstract

Background
The adverse effects of maternal diabetes on oocyte maturation and embryo development have been reported.

Methods
In this study, we used time-lapse live cell imaging confocal microscopy to investigate the dynamic changes of ER and the effects of diabetes on the ER’s structural dynamics during oocyte maturation, fertilization and early embryo development.

Results
We report that the ER first became remodeled into a dense ring around the developing MI spindle, and then surrounded the spindle during migration to the cortex. ER reorganization during mouse early embryo development was characterized by striking localization around the pronuclei in the equatorial section, in addition to larger areas of fluorescence deeper within the cytoplasm. In contrast, in diabetic mice, the ER displayed a significantly higher percentage of homogeneous distribution patterns throughout the entire ooplasm during oocyte maturation and early embryo development. In addition, a higher frequency of large ER aggregations was detected in GV oocytes and two cell embryos from diabetic mice.

Conclusions
These results suggest that the diabetic condition adversely affects the ER distribution pattern during mouse oocyte maturation and early embryo development.
Joint source and relay design for two-hop amplify-and-forward relay networks with QoS constraints
Authors: Mohammadi Jafar , Gao Feifei , Rong Yue , Chen Wen 
Published: April 2013

Abstract
In this paper, we consider the joint design of source precoding matrix and the relay precoding matrix in a two-hop multiple-input multiple-output relay network. The goal is to find a pair of matrices in order to minimize the power consumption and at the same time meet pre-selected quality of service constraints that are defined as the mean square error of each data stream. Using majorization theory, we simplify the matrix-valued optimization problem into a scalar-valued one. We then propose a lower bound and an upper bound of the original problem, both in convex forms. Specifically, the latter is solved by a multi-level water-filling algorithm that is much efficient than directly applying the interior point method. Numerical examples corroborate the proposed studies and also demonstrate the tightness of both bounds to the original problem.
Existence results for classes of infinite semipositone problems
Authors: Goddard Jerome , Lee Eun Kyoung , Sankar Lakshmi , Shivaji R 
Published: April 2013

Abstract
We consider the problem

{

−

Δ
p

u
=

a

u

p
−
1

−
b

u

γ
−
1

−
c

u
α

,

x
∈
Ω
,

u
=
0
,

x
∈
∂
Ω
,

where

Δ
p

u
=
div
(

|
∇
u
|

p
−
2

∇
u
)

,

p
>
1

, Ω is a smooth bounded domain in

R
n

,

a
>
0

,

b
>
0

,

c
≥
0

,

γ
>
p

and

α
∈
(
0
,
1
)

. Given a, b, γ and α, we establish the existence of a positive solution for small values of c. These results are also extended to corresponding exterior domain problems. Also, a bifurcation result for the case

c
=
0

is presented.
FPGA based wireless sensor node with customizable event-driven architecture
Authors: Liao Junsong , Singh Brajendra K , Khalid Mohammed AS , Tepe Kemal E 
Published: April 2013

Abstract

Abstract
This article presents the design and implementation of modular customizable event-driven architecture with parallel execution capability for the first time with wireless sensor nodes using stand alone FPGA. This customizable event-driven architecture is based on modular generic event dispatchers and autonomous event handlers, which will help WSN application developers to quickly develop their applications by adding the required number of event dispatchers and event handlers as per the need of a WSN application. This architecture can handle multiple events in parallel, including high priority ones. Additionally, it provides non-preemptive operation which removes the timing uncertainty and overhead involved with interrupt-driven processor-based sensor node implementation, which is required in real-time wireless sensor networks (WSNs). Thus, higher computation power of FPGAs combined with the non-preemptive modular event-driven architecture with parallel execution capability enables a variety of new WSN applications and facilitates rapid prototyping of WSN applications. In this article, the performance of FPGA-based sensor device is compared with general purpose processor-based implementations of sensor devices. Results show that our FPGA-based implementation provides significant improvement in system efficiency measured in terms of clock cycle counts required for typical sensor network tasks such as packet transmission, relay and reception.
Characteristics of Japanese Duchenne and Becker muscular dystrophy patients in a novel Japanese national registry of muscular dystrophy (Remudy)
Authors: Nakamura Harumasa , Kimura En , Mori-Yoshimura Madoka , Komaki Hirofumi , Matsuda Yu , Goto Kanako , Hayashi Yukiko K , Nishino Ichizo , Takeda Shin‘ichi , Kawai Mitsuru 
Published: April 2013

Abstract

Background
Currently, clinical trials for new therapeutic strategies are being planned for Duchenne and Becker muscular dystrophies (DMD/BMD). However, it is difficult to obtain adequate numbers of patients in clinical trials. As solutions to these problems, patient registries are an important resource worldwide, especially in rare diseases such as DMD/BMD.

Methods
We developed a national registry of Japanese DMD/BMD patients in collaboration with TREAT-NMD. The registry includes male Japanese DMD/BMD patients whose genetic status has been confirmed by genetic analysis. The registry includes patients throughout Japan.

Results
As of February 2012, 583 DMD and 105 BMD patients were registered. Most individuals aged less than 20 years. In terms of genetic mutations of registrants of DMD and BMD, deletion of exons was the most frequent (61.4% and 79.0%) followed by point mutations (24.5% and 14.3%) and duplications (13.6% and 4.8%), respectively. 43.6% of DMD are capable of walking, and 76.2% of BMD registrants are able to walk. 41.1% of DMD registrants in the database were treated using steroids. 29.5% of DMD and 23.8% of BMD registrants were prescribed one cardiac medicine at least. 22% of DMD used ventilator support, and non-invasive support was common. Small numbers of DMD and BMD registrants, only 3.9% and 1.0% of them, have received scoliosis surgery. 57 (9.8%) patients were eligible to clinical trial focused on ‘skipping’ exon 51.

Conclusions
The Remudy has already demonstrated utility in clinical researches and standardization of patients care for DMD/BMD. This new DMD/BMD patient registry facilitates the synchronization of clinical drug development in Japan with that in other countries.
In vivo measurement of apolipoprotein E from the brain interstitial fluid using microdialysis
Authors: Ulrich Jason D , Burchett Jack M , Restivo Jessica L , Schuler Dorothy R , Verghese Philip B , Mahan Thomas E , Landreth Gary E , Castellano Joseph M , Jiang Hong , Cirrito John R , Holtzman David M 
Published: April 2013

Abstract

Background
The APOE4 allele variant is the strongest known genetic risk factor for developing late-onset Alzheimer’s disease. The link between apolipoprotein E (apoE) and Alzheimer’s disease is likely due in large part to the impact of apoE on the metabolism of amyloid β (Aβ) within the brain. Manipulation of apoE levels and lipidation within the brain has been proposed as a therapeutic target for the treatment of Alzheimer’s disease. However, we know little about the dynamic regulation of apoE levels and lipidation within the central nervous system. We have developed an assay to measure apoE levels in the brain interstitial fluid of awake and freely moving mice using large molecular weight cut-off microdialysis probes.

Results
We were able to recover apoE using microdialysis from human cerebrospinal fluid (CSF) in vitro and mouse brain parenchyma in vivo. Microdialysis probes were inserted into the hippocampus of wild-type mice and interstitial fluid was collected for 36 hours. Levels of apoE within the microdialysis samples were determined by ELISA. The levels of apoE were found to be relatively stable over 36 hours. No apoE was detected in microdialysis samples from apoE KO mice. Administration of the RXR agonist bexarotene increased ISF apoE levels while ISF Aβ levels were decreased. Extrapolation to zero-flow analysis allowed us to determine the absolute recoverable concentration of apoE3 in the brain ISF of apoE3 KI mice. Furthermore, analysis of microdialysis samples by non-denaturing gel electrophoresis determined lipidated apoE particles in microdialysis samples were consistent in size with apoE particles from CSF. Finally, we found that the concentration of apoE in the brain ISF was dependent upon apoE isoform in human apoE KI mice, following the pattern apoE2>apoE3>apoE4.

Conclusions
We are able to collect lipidated apoE from the brain of awake and freely moving mice and monitor apoE levels over the course of several hours from a single mouse. Our technique enables assessment of brain apoE dynamics under physiological and pathophysiological conditions and in response to therapeutic interventions designed to affect apoE levels and lipidation within the brain.
Survival outcomes of patients with advanced oral cavity squamous cell carcinoma treated with multimodal therapy: A multi-institutional analysis
Authors: Zhang Han , Dziegielewski Peter T , Biron Vince L , Szudek Jacek , Al-Qahatani Khaled H , O'Connell Daniel A , Harris Jeffrey R , Seikaly Hadi 
Published: April 2013

Background The oral cavity is the most common site for head and neck squamous cell carcinoma. Treatment of advanced stage oral cavity squamous cell carcinoma (OCSCC) has classically involved surgical resection with postoperative adjuvant radiotherapy (S-RT).Despite this aggressive dual modality therapy, the disease outcomes have remained poor. The treatment options expanded in 2004 when two international trials showed the addition of postoperative chemotherapy to radiation improved outcomes. These trials were, however not oral cavity site specific. Objective To assess survival outcomes of advanced OCSCC treated by differing modalities. The primary goal was to determine if the addition of postoperative chemotherapy (S-CRT) improves survival compared to other treatment regimens. Methods Demographic, pathologic, treatment, and survival data was obtained from patients diagnosed with OCSCC from 1998–2010 in Alberta, Canada. 222 patients were included in the final analysis from 895 OCSCC patients. Actuarial overall, disease-specific, disease-free, and metastasis-free survivals were estimated with Kaplan-Meier and Cox regression analyses. Patients were grouped by treatment. Results Patients receiving S-CRT had improved overall, disease-specific, disease-free, and metastasis-free survival compared to S-RT, CRT or RT(p < 0.05). Two and five year estimated overall survival was significantly higher in the S-CRT group at 77 and 58% (p < 0.05), versus S-RT with 55 and 40% rates(p < 0.05). Results were similar for disease-specific, disease-free, and metastasis free survival with S-CRT being favoured. Patients with extracapsular spread (ECS) treated with S-CRT versus S-RT had 55% survival advantage at 5 years (p < 0.05). Conclusion This study shows that adding adjuvant chemotherapy to S-RT improves survival outcomes in advanced OCSCC, especially in patients with ECS.
In –silico molecular docking analysis of prodigiosin and cycloprodigiosin as COX-2 inhibitors
Authors: Krishna Pabba Shiva , Vani Kompally , Prasad Metuku Ram , Samatha Burra , Bindu Nidadavolu Shesha Venkata Sathya Si , Charya Maringanti Alaha Singara , Reddy Shetty Prakasham 
Published: April 2013

Abstract
Prodigiosin and cycloprodigiosin are tripyrrole red pigmented compounds with medical importance for their anticancer property. In the present investigation, molecular docking studies were performed for both prodigiosin and cycloprodigiosins to evaluate the in- silico anti-inflammatory activity against Cycloxigenase-2 (COX-2) protein as model compound and the data compared with rofecoxib and celcoxid. Cycloprodigiosin showed higher initial potential, initial RMS gradient and potential energy values compared to prodigiosin. Analysis of COX-2 protein and ligand binding revealed that cyclprodigiosin interacted with COX-2 protein amino acid residues of Tyr324, Phe487 and Arg89 while prodigiosin interaction was observed with two amino acids i.e. Leu321 and Tyr324. The computational ligand binding interaction suggested > 45% higher fitness score value for prodigiosin to that of cycloprodigiosin with COX-2 protein while the standard compounds rofecoxib and celecoxid revealed fitness score of 44 and 62, respectively. The prodigiosin ligand revealed the best fitness score compared with the standard drug rofecoxib suggesting the prodigiosin could be effective as the potential inhibitor compound against COX-2 protein and can be evaluated as anti-inflammatory drug molecule using clinical trials.
Rugby-ball-shaped Na0.5La0.5MoO4:Eu3+ 3D architectures: synthesis, characterization, and their luminescence behavior
Authors: Krishnan Rajagopalan , Thirumalai Jagannathan , Banu Ithrees Basha Shameem , Peter Anthuvan John 
Published: April 2013

Abstract
Three-dimensional self-assembled rugby-ball-shaped microarchitectures of tetragonal Na0.5La0.5MoO4:Eu3+ crystal structure were synthesized by facile hydrothermal method at 200°C for 8 h using ethylenediaminetetraacetic acid (EDTA) as surfactant. Structure and morphology of Na0.5La0.5MoO4:Eu3+ were investigated by X-ray diffraction and scanning electron microscopy. In the hydrothermal process, EDTA not only acts as a chelating reagent to facilitate the formation of Na0.5La0.5MoO4:Eu3+ but also acts as a surface capping agent to adhere to the newly created surface and to promote crystal splitting. The molar concentration of EDTA plays an important role and can effectively tune the size of the particles. Photoluminescence study reveals that hierarchical structures of Na0.5La0.5MoO4:Eu3+ show a strong emission in the red region under 395-nm UV excitation.
Management of bleeding and coagulopathy following major trauma: an updated European guideline
Authors: Spahn Donat R , Bouillon Bertil , Cerny Vladimir , Coats Timothy J , Duranteau Jacques , Fernández-Mondéjar Enrique , Filipescu Daniela , Hunt Beverley J , Komadina Radko , Nardi Giuseppe , Neugebauer Edmund , Ozier Yves , Riddez Louis , Schultz Arthur , Vincent Jean-Louis , Rossaint Rolf 
Published: April 2013

Abstract
Introduction
Evidence-based recommendations are needed to guide the acute management of the bleeding trauma patient. When these recommendations are implemented patient outcomes may be improved.

Methods
The multidisciplinary Task Force for Advanced Bleeding Care in Trauma was formed in 2005 with the aim of developing a guideline for the management of bleeding following severe injury. This document represents an updated version of the guideline published by the group in 2007 and updated in 2010. Recommendations were formulated using a nominal group process, the Grading of Recommendations Assessment, Development and Evaluation (GRADE) hierarchy of evidence and based on a systematic review of published literature.

Results
Key changes encompassed in this version of the guideline include new recommendations on the appropriate use of vasopressors and inotropic agents, and reflect an awareness of the growing number of patients in the population at large treated with antiplatelet agents and/or oral anticoagulants. The current guideline also includes recommendations and a discussion of thromboprophylactic strategies for all patients following traumatic injury. The most significant addition is a new section that discusses the need for every institution to develop, implement and adhere to an evidence-based clinical protocol to manage traumatically injured patients. The remaining recommendations have been re-evaluated and graded based on literature published since the last edition of the guideline. Consideration was also given to changes in clinical practice that have taken place during this time period as a result of both new evidence and changes in the general availability of relevant agents and technologies.

Conclusions
A comprehensive, multidisciplinary approach to trauma care and mechanisms with which to ensure that established protocols are consistently implemented will ensure a uniform and high standard of care across Europe and beyond.http://ccforum.com/content/17/4/442
Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation
Authors: Nelson Deirdre A. , Manhardt Charles , Kamath Vidya , Sui Yunxia , Santamaria-Pang Alberto , Can Ali , Bello Musodiq , Corwin Alex , Dinn Sean R. , Lazare Michael , Gervais Elise M. , Sequeira Sharon J. , Peters Sarah B. , Ginty Fiona , Gerdes Michael J. , Larsen Melinda 
Published: April 2013

Summary
Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor cell retention and expansion with cell differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching morphogenesis is the regulated proliferation and differentiation of perhaps several progenitor cell populations, which have not been characterized throughout development, and yet are critical for understanding organ development, regeneration, and disease. Here we applied a serial multiplexed fluorescent immunohistochemistry technology to map the progressive refinement of the epithelial and mesenchymal cell populations throughout development from embryonic day 14 through postnatal day 20. Using computational single cell analysis methods, we simultaneously mapped the evolving temporal and spatial location of epithelial cells expressing subsets of differentiation and progenitor markers throughout salivary gland development. We mapped epithelial cell differentiation markers, including aquaporin 5, PSP, SABPA, and mucin 10 (acinar cells); cytokeratin 7 (ductal cells); and smooth muscle α-actin (myoepithelial cells) and epithelial progenitor cell markers, cytokeratin 5 and c-kit. We used pairwise correlation and visual mapping of the cells in multiplexed images to quantify the number of single- and double-positive cells expressing these differentiation and progenitor markers at each developmental stage. We identified smooth muscle α-actin as a putative early myoepithelial progenitor marker that is expressed in cytokeratin 5-negative cells. Additionally, our results reveal dynamic expansion and redistributions of c-kit- and K5-positive progenitor cell populations throughout development and in postnatal glands. The data suggest that there are temporally and spatially discreet progenitor populations that contribute to salivary gland development and homeostasis.
Primary ciliogenesis requires the distal appendage component Cep123
Authors: Sillibourne James E. , Hurbain Ilse , Grand-Perret Thierry , Goud Bruno , Tran Phong , Bornens Michel 
Published: April 2013

Summary
Primary cilium formation is initiated at the distal end of the mother centriole in a highly co-ordinated manner. This requires the capping of the distal end of the mother centriole with a ciliary vesicle and the anchoring of the basal body (mother centriole) to the cell cortex, both of which are mediated by the distal appendages. Here, we show that the distal appendage protein Cep123 (Cep89/CCDC123) is required for the assembly, but not the maintenance, of a primary cilium. In the absence of Cep123 ciliary vesicle formation fails, suggesting that it functions in the early stages of primary ciliogenesis. Consistent with such a role, Cep123 interacts with the centriolar satellite proteins PCM-1, Cep290 and OFD1, all of which play a role in primary ciliogenesis. These interactions are mediated by a domain in the C-terminus of Cep123 (400–783) that overlaps the distal appendage-targeting domain (500–600). Together, the data implicate Cep123 as a new player in the primary ciliogenesis pathway and expand upon the role of the distal appendages in this process.
PSiP-Pincer Type Palladium-Catalyzed Dehydrogenative Borylation of Alkenes and 1,3-Dienes
Authors: Kirai Naohiro , Iguchi Shoichiro , Ito Tatsuyoshi , Takaya Jun , Iwasawa Nobuharu 
Published: April 2013

Abstract
Dehydrogenative borylation of alkenes and 1,3-dienes was realized by carrying out the reaction in the presence of bis(pinacolato)diboron (B2pin2) and a catalytic amount of PSiP-pincer palladium complex. This protocol has the following notable features. 1) Monoanionic nature of the PSiP-pincer ligand prevents the formation of boryl(hydrido)- or dihydridopalladium species, enabling synthesis of various vinyl- or dienylboronic esters in good yield from a 1:1 mixture of B2pin2 and alkenes or 1,3-dienes without forming hydroboration or hydrogenation products. 2) Due to the strong trans influence of the silicon atom, PSiP-pincer palladium complex showed high activity toward migratory insertion. 3) Suppression of these side-reactions and the high reactivity of the PSiP-pincer palladium complex enabled an efficient, successive dehydrogenative borylation to give 1,1- or 1,2-diborylated products depending on the kind of substituent on alkenes by using more than 2 equivalents of B2pin2. Mechanistic study revealed that PSiP-pincer borylpalladium complex was generated from hydridopalladium complex and B2pin2, and this complex underwent alkene insertion followed by β-hydride elimination to give alkenylboronic ester with regeneration of the hydridopalladium complex.
Comparison of arterial and venous blood biomarker levels in chronic obstructive pulmonary disease
Authors: Kelly Emer , Owen Caroline A , Abraham Amadeus , Knowlton David L , Celli Bartolome R , Pinto-Plata Victor 
Published: April 2013

Purpose: The development of novel biomarkers is an unmet need in chronic obstructive pulmonary disease (COPD). Arterial blood comes directly from the lung and venous blood drains capillary beds of the organ or tissue supplied. We hypothesized that there would be a difference in levels of the biomarkers metalloproteinase 9 (MMP-9), vascular endothelial growth factor A (VEGF-A) and interleukin 6 (IL-6) in arterial compared with venous blood. Methods: Radial artery and brachial vein blood samples were taken simultaneously in each of 12 patients with COPD and seven controls with normal lung function. Circulating immunoreactive MMP-9, VEGF-A and IL-6 levels in serum were measured using quantitative enzyme-linked immunosorbent assays. Results were compared using a Student’s paired t test. The study was powered to determine whether significant differences in cytokine levels were present between paired arterial and venous blood samples. Results: In the 12 patients with COPD, four were female, and age ranged 53-85 years, mean age 69 years. Three patients in the control group were female, with age range 46-84 years, mean age 64.7 years. In the COPD group, three patients had mild, five moderate and four severe COPD. No significant difference was found between arterial and venous levels of MMP-9, VEGF-A or IL-6. Conclusions: In this pilot study, levels of the measured biomarkers in arterial compared with venous blood in both COPD patients and healthy controls did not differ. This suggests that as we continue to chase the elusive biomarker in COPD as a potential tool to measure disease activity, we should focus on venous blood for this purpose.