bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel cisAB allele with a missense variant (c.971T>C) in the ABO gene of a Brazilian family
Authors: Miola Marcos Paulo , Prochaska Caroline Luise , Cardoso Guilherme , Ricci Junior Octávio , de Mattos Luiz Carlos 
Published: August 2024

AbstractBackground and ObjectivesMissense variants in exon 7 of the ABO gene can lead to the formation of cisAB alleles. These alleles encode glycosyltransferases (GTs) capable of synthesizing both A and B antigens. In this study, we report the discovery of a novel cisAB allele and characterize it at molecular, protein and serological levels.Materials and MethodsBlood and DNA samples from the proband and seven relatives were examined using standard and modified ABO phenotyping, polymerase chain reaction‐restriction fragment length polymorphism and ABO gene sequencing. We assessed the impact of the p.Leu324Ser variant on the protein structure of the mutant GT using bioinformatics tools.ResultsMolecular tests revealed a c.971T>C (p.Leu324Ser) variant in the ABO gene in five of the eight individuals. This variant results in a GT that produces more A antigens and fewer B antigens. Bioinformatics analysis suggests that the amino acid substitution (p.Leu324Ser) could potentially affect enzymatic activity and specificity of the GT.ConclusionWe identified a novel cisAB allele resulting from a c.971T>C variant in the ABO gene. This variant led to the expression of an ABweak phenotype.
Powering Toward Los Angeles: Comparing Power Output and Pacing Approach Between Maximal 2000- and 1500-m On-Water Racing in Elite Rowers
Authors: Astridge Daniel J. , Peeling Peter , Goods Paul S.R. , Girard Olivier , Binnie Martyn J. 
Published: August 2024

Purpose: To compare power output and pacing between maximal 1500- and 2000-m on-water rowing performances. Methods: Twenty-six (female n = 4, male n = 22) international rowers, across 6 boat classes, completed maximal 1500- and 2000-m on-water races, separated by 24 to 48 hours. Crew combinations and seat orders remained consistent between races. Peach PowerLine instrumentation measured power output and stroke rate. Differences in completion time, mean power output (MPO), percentage prognostic velocity (PPV; percentage of world record velocity in each boat class), stroke rate, and pacing variance were assessed using linear mixed modeling. Results: Compared with 2000-m, completion times were 90.4 (6.1) seconds shorter over 1500 m (−24.7% [0.7%]). Both MPO (P = .255, ) and PPV (P = .340, ) were not different between distances. Broadly, crews adopted a reverse-J-shaped pacing across both distances, demonstrating a reduced variance over 1500 m (P = .035, ). Percentage change in MPO from 2000 to 1500 m demonstrated a strong negative association with pacing variance over 1500 m (R2 = .74, P = .027). Conclusions: International rowing crews did not increase MPO or PPV when racing maximally over 1500 m compared to 2000 m. Comparable strategies were adopted over both distances, with less variance in pacing observed over 1500 m. The crews that demonstrated greater increases in MPO over the shorter race employed a flatter pacing strategy. To improve 1500-m on-water performance, rowers may need to adopt a more even pacing approach.
Interferon-stimulated genes and their antiviral activity against SARS-CoV-2
Authors: Ortega-Prieto Ana Maria , Jimenez-Guardeño Jose M. 
Published: August 2024

ABSTRACT

The coronavirus disease 2019 (COVID-19) pandemic remains an international health problem caused by the recent emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As of May 2024, SARS-CoV-2 has caused more than 775 million cases and over 7 million deaths globally. Despite current vaccination programs, infections are still rapidly increasing, mainly due to the appearance and spread of new variants, variations in immunization rates, and limitations of current vaccines in preventing transmission. This underscores the need for pan-variant antivirals and treatments. The interferon (IFN) system is a critical element of the innate immune response and serves as a frontline defense against viruses. It induces a generalized antiviral state by transiently upregulating hundreds of IFN-stimulated genes (ISGs). To gain a deeper comprehension of the innate immune response to SARS-CoV-2, its connection to COVID-19 pathogenesis, and the potential therapeutic implications, this review provides a detailed overview of fundamental aspects of the diverse ISGs identified for their antiviral properties against SARS-CoV-2. It emphasizes the importance of these proteins in controlling viral replication and spread. Furthermore, we explore methodological approaches for the identification of ISGs and conduct a comparative analysis with other viruses. Deciphering the roles of ISGs and their interactions with viral pathogens can help identify novel targets for antiviral therapies and enhance our preparedness to confront current and future viral threats.
Revisiting statefinder via Gaussian process
Authors: Feng Zhihua , Xu Lixin 
Published: August 2024

AbstractThe statefinder diagnostic is useful to discriminate dark energy models. In this paper, under the minimum assumption of a spatially flat Friedmann–Lemaître–Robertson–Walker Universe, we reconstruct the statefinder pair $$\{r(z),s(z)\}$$

{
r
(
z
)
,
s
(
z
)
}

in addition to the deceleration parameter q(z) via the Gaussian process from the cosmic observational data points which include the recently released Pantheon+ SN Ia samples and the observational Hubble data H(z). The reconstructed evolution trajectories are shown on the $$r-q$$

r
-
q

, $$r-s$$

r
-
s

planes. The reconstructed q(z) is consistent with previous studies. Meanwhile, a novel quantity named suitability is introduced on the $$r-q$$

r
-
q

, $$r-s$$

r
-
s

planes to assess the validation of a dark energy model.
Electrochemical Nanomolar Determination of the Anticancer Drug 5-Fluorouracil Using Chitosan Curcumin Schiff Base-Decorated NiO Nanorods Synthesized with Green Method
Authors: Abd-Elsabour Mohamed , Abou-Krisha Mortaga M. , Abdel-Haleem Fatehy M. , Goda Mohamed N. , Mohy-Eldin Mohamed S. 
Published: August 2024

5-Fluorouracil (5-FU) is widely used in the treatment of various cancers, necessitating accurate and sensitive detection techniques. Hybrid materials, combining organic and inorganic components, offer superior electrochemical characteristics, including enhanced conductivity and stability. Herein, NiO nanorods (NiONRs) were synthesized using Mangifera indica leaves extract and decorated with chitosan curcumin analog Schiff base (Cs-Cur-A). The characterization of the NiONRs and Cs-Cur-A were investigated through different techniques including FT-IR, 1HNMR, XRD, SEM, and EDX. A sensitive and selective electrochemical sensor to determine 5-FU was elucidated using a Cs-Cur-A/NiONRs modified glassy carbon electrode (GCE). The anodic peak current of 5-FU was greatly enhanced at the Cs-Cur-A/NiONRs/GCE with pH 7.0 and a scan rate of 50 mV s−1. According to chronoamperometric measurements, the value of diffusion coefficient (D) was estimated to be 2.96 × 10−6 cm2 s−1. Using differential pulse voltammetry (DPV), the fabricated sensor exhibits a wide linear range (0.1–150.0 μM) with a low limit of detection (21.75 nM). Moreover, the proposed sensor was successfully applied to the detection of 5-FU in blood serum and urine samples with high reproducibility, repeatability, and selectivity.
The Future of Government Pressure on Social Media Platforms
Authors: Volokh Eugene 
Published: August 2024

Abstract
As vast social media platforms undertake more content policing, the U.S. government has unsurprisingly tried to urge them to police things the way it prefers. This is likely to continue and, indeed, expand. What First Amendment constraints are there on such government pressure? This essay offers some tentative thoughts: 1) Some court of appeals cases have drawn lines distinguishing permissible attempts by government to persuade intermediaries to remove their users’ or business partners’ materials from impermissible government coercion. 2) The Supreme Court's employer free speech cases may also inform our understanding of what counts as subtle coercion. 3) Courts considering other constitutional rights, especially the Fourth Amendment, have concluded that even noncoercive government persuasion may sometimes constitute impermissible evasion of the constitutional mandate. 4) A recent appellate decision (which the Supreme Court vacated on procedural grounds) suggests a potential distinction between ad hoc and systematic attempts to persuade platforms to remove content, though whether that line is ultimately either sensible or administrable is an open question.
Risk and protective factors for revictimization from intimate partner violence: A systematic review
Authors: Ortega Pacheco Yesid José , Gallo-Barrera Yeison David , Pozo Muñoz Carmen 
Published: August 2024

Purpose: Intimate partner violence is a global public health problem that mainly affects women rather than men. It has been associated with negative physical and mental health outcomes, including experiences of revictimization. This systematic review describes the risk and protective factors associated with revictimization in female victims of intimate partner violence. Methods: We searched for articles published between 1995 and 2024 in the Scopus, Web of Science, and PsycINFO databases. In total, 925 studies that met the inclusion criteria were reviewed, of which 38 were evaluated as eligible, and 12 were finally included in the review. Results: Among the risk factors were mental health problems, some demographic characteristics of the victims, previous experiences of violence, and coping and attachment styles. Economic independence, empowerment, and restraining orders were protective factors. Conclusions: Based on the results, we present some practical implications for health and public policy as well as recommendations for directing future research into revictimization.
Comprehensive Management of Thrombosed Lower Extremity Venous Aneurysms: A Single-Center Retrospective Analysis and Insights Into Clinical Strategies
Authors: Alagha Sameh , Ekim Meral 
Published: August 2024

Objective Venous aneurysms are rare vascular abnormalities associated with venous thromboembolism. In this study, we presented our experience in managing thrombosed lower extremity venous aneurysms and evaluate the impact of vitamin D deficiency and genetic thrombophilic risk factors on patient management and outcomes. Methods A single-center retrospective observational analysis was conducted on ten patients with thrombosed lower limb venous aneurysms who underwent surgical procedures at our hospital from July 2014 to February 2023. Collected data included venous duplex ultrasonographic imaging and laboratory tests including genetic thrombophilic risk factors and assessments of vitamin D levels. Results There were 5 males and 5 females. The mean age was 46.6 ± 12.1 years. The aneurysms were located in the popliteal vein in three patients, the great saphenous vein in six, and the small saphenous vein in one. Venous duplex imaging revealed saccular aneurysms in eight patients and fusiform aneurysms in two, with a mean diameter of 37.2 ± 10.6 mm, ranging from 23 to 52 mm. Laboratory tests indicated vitamin D deficiency in all of the patients, and genetic thrombophilic risk factors were identified in two cases. Surgical interventions consisted of tangential excision with lateral venorrhaphy in three patients and total excision and ligation in seven patients. The postoperative period was favorable for all patients. Conclusions Surgical treatment of thrombosed venous aneurysms in the lower extremities is essential to prevent complications such as thromboembolism. A comprehensive approach, including knowledge of genetic risk factors and vitamin D levels, may improve patient outcomes.
The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
Authors: Lee Jangsoon , Kida Kumiko , Koh Jiwon , Liu Huey , Manyam Ganiraju C. , Gi Young Jin , Rampa Dileep R. , Multani Asha S. , Wang Jing , Jayachandran Gitanjali , Lee Dae-Won , Reuben James M. , Sahin Aysegul , Huo Lei , Tripathy Debu , Im Seock-Ah , Ueno Naoto T. 
Published: August 2024

Abstract
Background
Anti-HER2 therapies, including the HER2 antibody–drug conjugates (ADCs) trastuzumab emtansine (T-DM1) and trastuzumab deruxtecan (T-DXd), have led to improved survival outcomes in patients with HER2-overexpressing (HER2+) metastatic breast cancer. However, intrinsic or acquired resistance to anti-HER2–based therapies remains a clinical challenge in these patients, as there is no standard of care following disease progression. The purpose of this study was to elucidate the mechanisms of resistance to T-DM1 and T-DXd in HER2+ BC patients and preclinical models and identify targets whose inhibition enhances the antitumor activity of T-DXd in HER2-directed ADC-resistant HER2+ breast cancer in vitro and in vivo.

Methods
Targeted DNA and whole transcriptome sequencing were performed in breast cancer patient tissue samples to investigate genetic aberrations that arose after anti-HER2 therapy. We generated T-DM1 and T-DXd–resistant HER2+ breast cancer cell lines. To elucidate their resistance mechanisms and to identify potential synergistic kinase targets for enhancing the efficacy of T-DXd, we used fluorescence in situ hybridization, droplet digital PCR, Western blotting, whole-genome sequencing, cDNA microarray, and synthetic lethal kinome RNA interference screening. In addition, cell viability, colony formation, and xenograft assays were used to determine the synergistic antitumor effect of T-DXd combinations.

Results
We found reduced HER2 expression in patients and amplified DNA repair–related genes in patients after anti-HER2 therapy. Reduced ERBB2 gene amplification in HER2-directed ADC–resistant HER2+ breast cancer cell lines was through DNA damage and epigenetic mechanisms. In HER2-directed ADC–resistant HER2+ breast cancer cell lines, our non-biased RNA interference screening identified the DNA repair pathway as a potential target within the canonical pathways to enhance the efficacy of T-DXd. We validated that the combination of T-DXd with ataxia telangiectasia and Rad3-related inhibitor, elimusertib, led to significant breast cancer cell death in vitro (P < 0.01) and in vivo (P < 0.01) compared to single agents.

Conclusions
The DNA repair pathways contribute to HER2-directed ADC resistance. Our data justify exploring the combination treatment of T-DXd with DNA repair–targeting drugs to treat HER2-directed ADC–resistant HER2+ breast cancer in clinical trials.
Harnessing surface plasmon polaritons from nanogroove plasmonic lenses to enhance the EM fields around plasmonic nanoantennas
Authors: Gupta Nitin , Dhawan Anuj 
Published: August 2024

In this paper, we present a device—consisting of a bowtie nanoantenna surrounded by either an asymmetric plasmonic nanogroove or a symmetric plasmonic nanogroove—that allows improved excitation of the bowtie nanoantenna via interaction between the bowtie nanoantenna and the surface plasmon polaritons (SPPs) generated from the nanogrooves. We study the effect of both a linear plasmonic nanogroove as well as a circular plasmonic nanogroove (i.e., a circular nanogroove plasmonic lens) on the EM fields around a bowtie nanoantenna placed at a certain distance from the nanogroove. We show that the electric field enhancement of a bowtie nanoantenna can be significantly improved by the interaction between the bowtie nanoantenna and the SPPs generated from the nanogrooves. We employed FDTD simulations to calculate quantities such as the electric field enhancements and power coupling into SPPs as well as a finite difference eigenmode solution to obtain dispersion relation of the nanogrooves. The geometrical parameters of the symmetric nanogroove and asymmetric nanogroove were optimized to couple maximum light into SPPs. We show that the bowtie nanoantenna surrounded by a single asymmetric nanogroove plasmonic lens produces a SERS electromagnetic enhancement factor (EMEF) of 1010—even when the gap between the arms of the bowtie nanoantenna is as large as 10 nm—which is three orders of magnitude higher than SERS EMEF of a stand-alone bowtie nanoantenna and one order of magnitude higher than the SERS EMEF of a bowtie nanoantenna surrounded by a single symmetric nanogroove plasmonic lens. In addition, the effect of the radius of nanogroove plasmonic lenses is studied. The calculation of collection efficiencies of Raman signal from the proposed nanostructures shows that ∼62% Raman signal can be collected from the bowtie nanoantenna surrounded by a symmetric nanogroove plasmonic lens or an asymmetric nanogroove plasmonic lens (PL) compared to 10% Raman signal collected from only a bowtie nanoantenna on a silica substrate.
Correction of laser sweeping nonlinearities using ultralow-loss on-chip 7  m spiral resonators
Authors: Terra Osama , Jin Warren , Kotb Hussein , Guo Joel , Bowers John E. 
Published: August 2024

Swept laser interferometry is an extremely powerful solution embedded in several recent technologies such as absolute distance measurement, light detection and ranging (LiDAR), optical frequency domain reflectometry, optical coherence tomography, microresonator characterization, and gas spectroscopy. Nonlinearity in the optical frequency sweeping of tunable lasers is a fatal drawback in gaining the expected outcome from these technologies. Here, we introduce an on-chip, millimeter-scale, 7 m spiral resonator that is made of ultralow-loss Si3N4 to act as a frequency ruler for correction of the tunable lasers sweeping nonlinearities. The sharp 2 MHz frequency lines of the 8.5×107 high-quality factor resonator and the narrow-spaced 25.566 MHz frequency ticks of the 7 m spiral allow unprecedented precision for an on-chip solution to correct the laser sweeping nonlinearity. Accurate measurements of the ruler’s frequency spacing, linewidth, and temperature and wavelength sensitivities of the frequency ticks are performed here to demonstrate the quality of the frequency ruler. In addition, the spiral resonator is implemented in an frequency-modulated continuous-wave LiDAR experiment to demonstrate a potential application of the proposed on-chip frequency ruler.
Oxford handbook of evidentiality (A. Aikhenvald)
Authors: Zubeldia Larraitz 
Published: August 2024

Hizkuntza guztiek dituzte hiztunak bera ematen ari den informazioaren iturria zein den adierazteko hizkuntza-baliabideak. Denek dituzte era bateko edo besteko bide lexikalak (aditzondoak, izenak, partikulak eta abar) informazioa nondik datorren adierazteko. Munduko hizkuntzen laurdenak, ordea, bide horiez gain, markatzaile gramatikalak ere baditu informazio-iturria markatzeko (Aikhenvald 2004b); hau da, aditzarekin batera nahiz aditzaren gunean agertzen diren elementuak. Ebidentzial esaten zaie gisa horretako baliabideei, eta ebidentzialitatea kategoriari. Hala ere, ez dago erabateko adostasunik ebidentzialitatea definitzean edo zedarritzean. Nolanahi ere, definizio orokor bat emate aldera, ebidentzialitatea esaten zaio hizkuntzan informazio-iturria markatzeko bitartekoak biltzen dituen kategoriari. Kategoria finkatzetik beretik hasten dira desadostasunak, ordea. Mota bateko ikuspegiaren arabera, bide lexikalak eta gramatikalak, denak sartzen dira ebidentzialitatean; beste mota bateko ikuspegiaren arabera, berriz, markatzaile gramatikalak bakarrik. Batez ere liburu honen editore Aikhenvalden —eta beste egile batzuen— ustez, informazio-iturria gramatikalki adierazteari bakarrik esan behar litzaioke ebidentzialitatea. Bera da ikuspegi horren defendatzaile sutsua; Squartinik (2018), lan honetako bere artikuluan, adierazten duen bezala, bera da ikuspegi horren ordezkaria. Beste batzuen arabera (ikus, besteak beste, Narrog 2005, Cornillie 2007, McCready eta Ogata 2007, Squartini 2007 eta Boye 2010), aldiz, ikuspegi murritzegia da hori, terminoaren definiziotik kanpora uzten baititu hala hainbat hizkuntza, nola hizkuntza horietan hiztunaren informazio-iturria adierazteko erabiltzen diren hainbat bitarteko. Badirudi, dena dela, azken urteetan nolabaiteko adostasuna lortu dela, eta bi multzo nagusitan banatzen dira informazio-iturria adierazteari lotutako hizkuntza-baliabideak: elementu ebidentzialak (gramatikalak) eta estrategia ebidentzialak (bide lexikalak).
The Effect of Different Ovarian Storage Times and Oocyte Collection Methods on the Quantity, Quality, and Maturation of Buffalo Oocytes In Vitro
Authors: Tahuk Paulus Klau , Nahak Oktovianus R. , Bira Gerson Frans 
Published: August 2024

This study aimed to ascertain the impact of oocyte harvest methods and storage times on the amount, quality, and rate of buffalo oocyte maturation in vitro. Ovaries were collected from slaughterhouse at four different time storages (0, 3, 6, 9 and 12 h) before processing with three collection techniques slicing, aspiration, and slicing plus the aspiration of oocytes. According to the morphology of cumulus cell layers closely attached to the zona pellucida of the oocytes and the cytoplasmic appearance of the oocytes, the oocytes were divided into 4 groups (A, B, C, and D). Cumulus-oocyte-complexes (COCs) were transferred into in vitro maturation (IVM) medium of TCM- 199 (Sigma) for 24 h. The stages of nuclear maturation were examined by staining the enlarged COCs from each group. The result found that the average quantity of oocytes per ovary ranged from 8.08±1.28 to 9.83 ±1.89 and no significant effect (P0.05) on the quantity of oocytes per ovary. The average of oocytes quality per ovary ranges from 0.2 to 5.00 was a highly significant effect of storage time on quality oocytes (P0.01). The maturation rate in the M-II stage ranges from 1.33 to 3.92 per ovary, and storage time is a significant effect (P0.05) on the maturation rate of local buffalo. The collection methods were highly significant (P0.01) on the quantity of oocytes between slicing and aspiration, also between aspiration plus slicing, and no significant (P0.05) between slicing and aspiration plus slicing. The Average oocyte quality ranged from 0.10 to 4.95 per ovary and the highest in slicing plus aspiration methods (P0.05). The average maturation rate in M-II stage was ranged from 1.00 to 3.85 oocytes per ovary, and the methods of oocytes collection were significant (P0.05) on the maturation rate of local buffalo. In conclusion that the storage time of up to 6 h and aspiration plus slicing is suitable on in vitro maturation of local buffalo.