bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel E2F/Sphingosine Kinase 1 Axis Regulates Anthracycline Response in Squamous Cell Carcinoma
Authors: Hazar-Rethinam Mehlika , de Long Lilia Merida , Gannon Orla M. , Topkas Eleni , Boros Samuel , Vargas Ana Cristina , Dzienis Marcin , Mukhopadhyay Pamela , Simpson Fiona , Endo-Munoz Liliana , Saunders Nicholas A. 
Published: November 2014

Abstract
Purpose: Head and neck squamous cell carcinomas (HNSCC) are frequently drug resistant and have a mortality rate of 45%. We have previously shown that E2F7 may contribute to drug resistance in SCC cells. However, the mechanism and pathways involved remain unknown.
Experimental Design: We used transcriptomic profiling to identify candidate pathways that may contribute to E2F7-dependent resistance to anthracyclines. We then manipulated the activity/expression of the candidate pathway using overexpression, knockdown, and pharmacological inhibitors in in vitro and in vivo models of SCC to demonstrate causality. In addition, we examined the expression of E2F7 and a downstream effector in a tissue microarray (TMA) generated from HNSCC patient samples.
Results: E2F7-deficient keratinocytes were selectively sensitive to doxorubicin and this was reversed by overexpressing E2F7. Transcriptomic profiling identified Sphingosine kinase 1 (Sphk1) as a potential mediator of E2F7-dependent drug resistance. Knockdown and overexpression studies revealed that Sphk1 was a downstream target of E2F7. TMA studies showed that E2F7 overexpression correlated with Sphk1 overexpression in human HNSCC. Moreover, inhibition of Sphk1 by shRNA or the Sphk1-specific inhibitor, SK1-I (BML-EI411), enhanced the sensitivity of SCC cells to doxorubicin in vitro and in vivo. Furthermore, E2F7-induced doxorubicin resistance was mediated via Sphk1-dependent activation of AKT in vitro and in vivo.
Conclusion: We identify a novel drugable pathway in which E2F7 directly increases the transcription and activity of the Sphk1/S1P axis resulting in activation of AKT and subsequent drug resistance. Collectively, this novel combinatorial therapy can potentially be trialed in humans using existing agents. Clin Cancer Res; 21(2); 417–27. ©2014 AACR.
Dietary Diindolylmethane Suppresses Inflammation-Driven Lung Squamous Cell Carcinoma in Mice
Authors: Song Jung Min , Qian Xuemin , Teferi Fitsum , Pan Jing , Wang Yian , Kassie Fekadu 
Published: November 2014

Abstract
Inflammatory conditions of the lung such as chronic obstructive pulmonary disease (COPD) are known to increase lung cancer risk, particularly lung squamous cell carcinoma (LSCC). In the present study, we developed a mouse model of inflammation-driven LSCC that was induced by N-nitroso-trischloroethylurea (NTCU) and enhanced by lipopolysaccharide (LPS), a potent proinflammatory agent contained in tobacco and tobacco smoke, and determined the chemopreventive effects of BioResponse diindolylmethane (DIM) in the same model. Compared with mice treated with NTCU alone, mice treated with the combination of NTCU and LPS had a 9-fold increase in the number of bronchioles with LSCC. Also, compared with mice treated with LPS alone, mice treated with NTCU plus LPS showed significantly increased expression of the inflammatory cytokines IL1α, IL6, and TNFα (all three increased about 7-fold). Parallel to the increased cytokine gene expression, the NTCU plus LPS-treated group exhibited significantly enhanced activation of NF-κB, STAT3, ERK, p-38, and Akt, expression of p53, COX-2, and Mcl-1, and NF-κB- and STAT3-DNA binding in the lung. Dietary administration of DIM (10 μmol/g diet or 2,460 ppm) to mice treated with NTCU plus LPS reduced the incidence of LSCC by 2-fold, suppressed activation/expression of proinflammatory and procarcinogenic proteins and NF-κB- and STAT3-DNA binding, but not the expression of cytokines and p53. This study highlights the potential significance of our mouse model to identify promising drugs or dietary agents for the chemoprevention of human LSCC and that DIM is a very good candidate for clinical lung cancer chemoprevention trials. Cancer Prev Res; 8(1); 77–85. ©2014 AACR.
Communal Liberties and Fragmented Sovereignty
Authors: Krom Mikhail 
Published: November 2014

The paper’s basic premise is that the fate of a medieval city commune was determined to a large extent by its status in a bigger political system. Moreover, most of European towns and cities had only, to use Charles Tilly’s term, “fragmented sovereignty” when the control over the city was divided between several authorities including the city magistrates and a prince or an emperor. It means that city republics should be studied in their political settings, i.e., through the lens of their relations with the other powers in the region.
In the paper, such an “environmental” approach is applied to fifteenth-century Pskov, a typical “fragmented sovereignty”, which was dependent on the Novgorod archbishop and on Russian metropolitan in ecclesiastic affairs and belonged to the Grand Principality of Vladimir and Moscow in secular politics. Medieval Pskov had its own judicial system and legislation resembling that of some German cities and until the 1460s even enjoyed the right to invite and expel princes who had been turned into the city magistrates. But as the emerging Muscovite state tightened its control over North Western Russian lands in the second half of the fifteenth century, Pskov’s liberties were gradually reduced. The fate of Pskov finally absorbed by the Muscovite monarchy in 1510 was not unique: the Russian city followed the path of many other communes in different parts of Europe where emerging early modern sovereign states put an end to local autonomies and various “fragmented sovereignties”.
Transphyseal Anterior Cruciate Ligament Reconstruction in Patients With Open Physes
Authors: Calvo Rafael , Figueroa David , Gili Federico , Vaisman Alex , Mocoçain Pablo , Espinosa Maximiliano , León Agustín , Arellano Sergio 
Published: November 2014

Background: The treatment of anterior cruciate ligament (ACL) injuries in skeletally immature patients is controversial. Current evidence supports the view that surgical techniques restore knee stability and prevent progressive articular damage. However, most of the studies on this topic are small case series or they have short- or medium-term follow-up times. Purpose: To determine the long-term functional outcomes and secondary complications of transphyseal intra-articular ACL reconstruction with hamstring graft in skeletally immature patients. Study Design: Case series; Level of evidence, 4. Methods: Transphyseal ACL reconstruction with autograft hamstrings was performed in 27 skeletally immature patients. The average age at surgery was 13 years (range, 12-16 years), and the average follow-up time was 10.6 years (range, 10-13 years). Clinical outcomes were assessed with preoperative and final follow-up Tegner, International Knee Documentation Committee (IKDC) subjective, and Lysholm scores. Surgery details, return to sports, and ACL reconstruction failures were collected. The anteroposterior knee laxity was assessed by arthrometry, and the presence of deformities and lower limb length discrepancies were evaluated by radiographs. The presence of degenerative signs on anteroposterior and lateral knee radiographs at final follow-up was also evaluated. Results: Transphyseal ACL reconstruction was performed with vertically oriented tunnels, 7 to 10 mm in diameter, using semitendinosus-gracilis autograft. The average preoperative Tegner, IKDC, and Lysholm scores were 7, 55, and 40, respectively. Significant differences in these scores were observed at the time of the final assessment (Tegner, 6 [ P = .026]; IKDC, 94 [ P < .001]; Lysholm, 92 [ P < .001]). Two patients reported instability during sports activity. Three patients had a rupture of the ACL graft. No leg length discrepancy, axis malalignment, or degenerative changes were observed. Conclusion: The transphyseal ACL reconstruction in skeletally immature patients is a safe option, with high functional and satisfaction results, without significant growth plate damage in this series of patients.
Talectomy and Tibiocalcaneal Arthrodesis With Intramedullary Nail Fixation for Treatment of Equinus Deformity in Adults
Authors: Gursu Sarper , Bahar Hakan , Camurcu Yalkin , Yildirim Timur , Buyuk Fettah , Ozcan Cagri , Sahin Vedat 
Published: November 2014

Background: Severe equinovarus foot deformity in adults is a challenging problem. Conservative treatment rarely is effective, and operative options are limited. The aim of this study was to evaluate the results of talectomy and tibiocalcaneal arthrodesis with intramedullary nail fixation for the treatment of severe equinovarus deformity in adults. Methods: Twelve patients (average age 39 years, range 15-70 years) with severe equinovarus deformities of the foot were treated with talectomy and tibiocalcaneal arthrodesis with intramedullary nail fixation between March 2010 and February 2013. Average follow-up was 20 months (range 10-37 months). Results: Tibiocalcaneal fusion was achieved in all patients at an average of 12 weeks (range 8-17 weeks). Preoperatively, all patients had severe, irreducible equinovarus deformities; at last follow-up, almost all feet had mild residual deformity, but were plantigrade and did not require a brace or orthosis. The average AOFAS ankle score improved from 41.1 (range 8-66) preoperatively to 78.4 (range 67-86) postoperatively ( P = .02). There was a similar improvement in the average VAS score from 6.3 (range 2-10) preoperatively to 0.8 (range 0-4) postoperatively ( P = .02). Conclusion: The combination of talectomy and tibiocalcaneal arthrodesis was effective in correcting severe rigid equinovarus deformity in adults. Removal of the talus resulted in laxity of the soft tissues, making correction of the deformity easier. Tibiocalcaneal arthrodesis achieved a stable foot without the problems associated with talectomy alone. Level of Evidence: Level IV, case series.
Serological Survey of Dengue Virus Immunoglobulin M Among Febrile Patients in Kaduna Metropolis, Nigeria
Authors: Idoko Matthew O. , Ado Saleh A. , Umoh Veronica J. 
Published: November 2014

The purposes of this research were to determine the prevalence of dengue virus in the study population, to determine the relationship between the occurrence of dengue virus IgM and potential risk factors and to determine the association between the occurrence of dengue virus and some symptoms. In this study, blood specimen were collected from 340 subjects with febrile complaints attending hospitals in Kaduna metropolis, Nigeria and who gave their consent to participate. Serological determination of dengue virus IgM was carried out using enzyme linked immunosorbent assay technique. Structured questionnaires were also administered to obtain data on demographic, risk factors, and symptoms of illness. A total of 6 subjects were positive for dengue virus IgM giving a prevalence of 1.8%. There was no statistically significant association between the occurrence of dengue virus IgM and potential risk factors, demographic factors and symptoms of the illness. The occurrence of dengue virus in the study population was confirmed.
Emotion regulation and adolescent suicide: a proposal for physician education
Authors: Rice Timothy R. 
Published: November 2014

Abstract
Suicide is the second leading cause of death among adolescents aged 14–19 years. Pediatricians report strong interest in receiving additional training to reduce suicide mortality, and physician education is one of the most robust means of suicide prevention. However, many studies suggest that existing educational methods and means leave much room for improvement. In light of the emerging evidence that emotion regulation (ER) deficits are significantly associated with adolescent suicide, this paper proposes the untested hypothesis that a module on the brain-based ER system may strengthen existing methods of provider education. The ER system and the evidence supporting its association with adolescent suicide are reviewed. The ability to ground an approach to suicide prevention within this brain-based medical model may be appealing to pediatricians; its transdiagnostic breadth and dimensional makeup may also be appealing to pediatricians. Most importantly, its emphasis on the negative effects of impoverished self-regulation broaden non-specialist concern from a restriction upon withdrawn, depressed adolescents to those with a wide range of psychopathology. Implications and further considerations are discussed.
The symbology of the sea in Lygia Bojunga Nunes's work: a reflection on utopian and dystopian framing
Authors: Ferreira Eliane Aparecida Galvão Ribeiro , Matsuda Alice Atsuko 
Published: November 2014

The ambivalent image of the sea is recurrent in Bojunga Nunes’s work. It receives a lot of symbolic meanings in her production, being sometimes a positive and sometimes a negative element. Thus, the sea is used in a utopic way, as a place for pleasure and leisure, and in a dystopic way, as scenery of a rite of passage, which includes the pain and suffering, part of the rite of passage that characterizes the bildungsromans. So, if in some texts the sea assumes a utopic character, being a place of snuggle, security, the ideal to reach, in others it is represented as dystopic, a place of distress, asphyxia and death. In this text we will study the hypothesis that, even in its dark symbolic meaning, in the author’s work the sea is an element of reflection and revelation. Thereby, it assumes metaphorically a sense of magnification of the reader’s expectative, being an element of emancipation. The objective of this text is to present a reflection on the sea’s symbolism on the mentioned texts and its importance for the reader’s formation.DOI http://dx.doi.org/10.15304/elos.1.1720
Reflexos de Alice: Corpo, Gênero e escrita em Alice no Espelho, de Laura Bergallo
Authors: Barbosa Carrijo Silvana Augusta 
Published: November 2014

Por via de aportes teóricos vários, porque interdisciplinar se nos apresenta a produção epistemológica voltada ao exame do gênero literário infantil e juvenil, importa investigar a obra Alice no espelho (2005), de Laura Bergallo, com ilustrações de Edith Derdyk. Na narrativa juvenil em questão, a autora contempla a história de Alice, uma adolescente que desenvolve um processo doloroso e angustiante de anorexia nervosa. A partir de um intenso e constante processo de intertextualidade com o clássico de Lewis Carroll, a escritora trata de forma bastante literária o tema, em muito distanciando sua obra de um mero compêndio médico dos sintomas e do tratamento da doença. Por via do presente trabalho, objetivamos investigar a representação literária do corpo e o investimento intertextual realizado pela autora como um dos procedimentos estilísticos que conferem literariedade à obra.DOI http://dx.doi.org/10.15304/elos.1.1727
O ciclo do lobo e a raposa nos contos de animais
Authors: Ferreira Boo Mª del Carmen 
Published: November 2014

Despois de describir os contos de animais, unha das clases na que os especialistas dividen os contos da transmisión oral, e de presentar as súas diferentes funcións, o estudo céntrase no denominado "ciclo do lobo e a raposa". Expóñense as súas características que se exemplifican co análise dos contos deste tipo que se reproducen na obra Contos de animais I, de Xosé Miranda, Antonio Reigosa e Xoán R. Cuba, comentando os tipos e motivos e as diferenzas entre as versións orais e as reescrituras que se ofrecen na colección infantil.DOI http://dx.doi.org/10.15304/elos.1.1756
“Sozinho contra o mundo inteiro": representações da infância e da adolescência em José Marmelo e Silva
Authors: Ramos Ana Margarida 
Published: November 2014

Pretende-se, neste estudo, proceder à análise das representações da infância e da adolescência em duas obras narrativas do escritor português José Marmelo e Silva. O tema da infância e da adolescência reveste-se de especial importância no contexto da literatura portuguesa do século XX, permitindo a reflexão sobre o contexto social e político, mas tambo e Silva; Portuguese literatureteratura; social critique dele com as crianças. Tambereender o livrortado para estas questém económico e educativo, que enquadrava o crescimento. No conto “Narrativa bárbara” e na novela Adolescente Agrilhoado é visível a recriação literária do processo de crescimento e de afirmação identitária do adolescente, em contextos adversos e hostis.DOI http://dx.doi.org/10.15304/elos.1.1784
Field study on swine influenza virus (SIV) infection in weaner pigs and sows
Authors: Meiners C. , Loesken S. , Doehring S. , Starick E. , Pesch S. , Maas A. , Noe T. , Beer M. , Harder T. , grosse Beilage E. 
Published: November 2014

SummaryObjective: The aim of this field study was to explore the occurrence of and factors associated with the detection of swine influenza virus (SIV) by RTqPCR in weaner pigs and sows from herds with a history of respiratory or reproductive disorders. Material and methods: The sample set was based on nasal swabs from 823 sows (123 submissions) and 562 weaner pigs (80 submissions). Nasal swab samples were taken and submitted by 51 veterinary practices from all over Germany. Corresponding to the pig density most of the submissions ori ginated from the north-western part of Germany. The nasal swabs were used to detect SIV RNA by real-time RT-PCR (RTqPCR). Subtyping of SIV RNA by conventional RT-PCR and sequencing was attempted directly from clinical samples or from isolates when available. The herd characteristics, management and housing conditions of the pig herd as well as the course of the disease were collected by a telephone questionnaire with the herd attending veterinarian. Results: SIV was detected by RTqPCR in 53.8% of the submissions from weaner pigs with a history of respiratory disease. Moreover SIV was detected in 10.6% of the submissions from sows. The predominant endemic subtype found in nasal swabs from sows and weaner pigs was H1N1 (60.5%) whereas subtypes H1N2 (14.0%) and H3N2 (14.0%) were detected less frequently. In addition, human pandemic H1N1 virus or reassortants thereof were found in 11.5%. Conclusion and clinical relevance: The results underline the significance of a SIV infection in young pigs. A significant lower detection of SIV in weaner pigs was associated with the vaccination of piglets against porcine circovirus type 2 (PCV2), possibly indicating an interaction of SIV and PCV2. Most of the positive samples from sows originated from gilts, whereas only two originated from sows. An association between reproductive disorders and the detection of SIV could not be confirmed.
Distinct Reactivity of Transient Receptor Potential Vanilloid Subtype 1 in a Murine Model of Atopic Dermatitis with Serious Scratching
Authors: Xia Yan , Tanaka Akane , Oida Kumiko , Matsuda Akira , Jang Hyosun , Amagai Yosuke , Ishizaka Saori , Matsuda Hiroshi 
Published: November 2014

Background:
Abnormality in skin sensitivity may be responsible for unbearable itch in patients with atopic
dermatitis (AD).

Objectives:
We evaluated reactivity of NC/Tnd mice, a model for human AD, against various experimental stimulations.

Methods:
Several behavioral tests were performed after external stimuli were applied to NC/Tnd mice. Transient receptor
potential vanilloid subtype 1 (TRPV1) reactivity of neuronal cells collected from the dorsal root ganglions (DRG) was
analyzed with a Ca++ influx test. Finally, we evaluated suppressive effect of capsaicin on atopic itch of NC/Tnd mice.

Results:
Pain responses to heat, acidic stimulation, and capsaicin injection, which are transduced through TRPV1, were
decreased in NC/Tnd mice, when compared to two standard strains BALB/c and C57BL/6 mice. The reactivity of the
primary neurons isolated from DRG to capsaicin was markedly reduced in NC/Tnd mice. Topical application of histamine
evoked scratching in NC/Tnd mice as well as other two strains; however, the scratching intensities induced by nonhistamine
pruritogens were significantly lower in NC/Tnd mice comparing to the two strains. In conventional NC/Tnd
mice with AD, topical application of capsaicin reduced the scratching behavior.

Conclusion:
TRPV1 is associated with both pain and itch sensation; however, abnormalities in TRPV1 reactivity may involve
in severe itch in NC/Tnd mice.
Acute Myocardial Infarction with an Initially Non–Diagnostic Electrocardiogram – Clinical Intuition is Crucial for Decision Making
Authors: Petrović Dejan , Ilić Marina Deljanin , Stojanović Sanja , Stoičkov Viktor 
Published: November 2014

Summary
Acute myocardial infarction (AMI) is a common cause of reduced cardiac capacity and functional status of a patient. Successful primary percutaneous coronary intervention (pPCI) in acute coronary syndrome and appropriate, complex cardiovascular rehabilitation play a major role in preserving left ventricular function and improvement of prognosis and quality of patient’s life. The aim of this paper was to present a clinical course in a patient with acute myocardial infarction who did not have a classic ECG presentation in the form of ST-segment elevation. We showed that in everyday clinical work it is essential to timely recognize the symptoms of acute myocardial infarction, make the proper diagnosis and perform right treatment strategy. The next step is cardiovascular rehabilitation program, which positive effects, in our patient, was achieved through the retrieval of functional capacity and correction of risk factors.
Preliminary Researches Regarding the Conservation of Endangered Local Cattle to be Abandoned and the Achieving of Meat Hybrids Throughout the Hybridization with Romanian Breeds
Authors: Dascălu L. , Creangă Şt. , Borş I. , Ruginosu Elena , Postolache Alina Narcisa 
Published: November 2014

Abstract
Studies were performed on first generation meat hybrids, from the Bălţată cu Negru Românească (BNR) dairy cattle, into four experimental groups of five animals /group, in relation to meat bulls breeds from which the frozen semen material used for cows artificial insemination, respectively: Group E1- Limousine (Li), E2 - Aberdeen Angus (AA), E3 - Blue Blanch Belgian (BBB) and E4 - Charolaise (CH). The hybrids obtained from the experimental groups were observed in terms of dynamic growth and development through biometric measurements and periodic weightings 3 months intervals until 12 months of age. Average Daily Gain (ADG) recorded different variations in report to age and breed: at six months old, between 418.52 ± 60.97 g (Group E3 -BBB) and 486.67 ± 108.66 g (Group E2 - AA), at 12 months old, between 997.22 g (Group E2 - AA) and 1311.11 g (Group E3 -BBB). Slaughter rate at 12 months showed variations in relation to breed and thermal regime, so in hot regime the values ranged between 56.62 % (BNR x AA) and 60.60 % (BNR x BBB) and in cold regime (to 24 hours) between 55.68 % (BNR x AA) and 59.73 % (BNR x BBB). Carcasses development indicators had values in most cases in favor of BNR x AA hybrids than BNR x BBB hybrids, with significant differences (P ≤ 0.01) regarding the width index (201.43 cm to 175.84 cm) and pulp development index (129.27 cm to 110.64 cm). From results analysis it can be concluded that in dangered local cattle, to be abandoned because of productive reform or not relevant point of view of milk production can be used in first generation for hybridization with meat breeds in obtaining meat hybrids with morphological features, slaughter rate and carcass structure from superior quality categories than the maternal breed.