bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can Non-specific Blood Inflammatory Biomarkers Predict Recovery in Acute Transient Psychotic Disorder? A Prospective Observational Study
Authors: Prakash Jaya , Kar Sujita Kumar , Ali Wahid , Dalal Pronob Kumar 
Published: February 2025

Background: Inflammation may play an important role in the pathogenesis of schizophrenia and related psychotic disorders. This study aimed to measure the inflammatory marker (serum prolactin, serum cortisol, C-reactive protein [CRP], erythrocyte sedimentation rate (ESR), total leucocyte count, and differential leucocyte count) in drug naïve patients of the first episode of acute and transient psychotic disorder (ATPD) at baseline and their alterations after three months of antipsychotic treatment. Methods: This prospective study estimated the change of inflammatory biomarkers in patients with ATPD over three months, along with the change in psychopathology and global functioning. Attempts were taken to compare the trend of change in inflammatory biomarkers with clinical improvement. Result: A total of 157 patients with ATPD were screened to recruit 78 patients, of which only 33 patients were followed up till three months from the point of recruitment. More than three-fourths of the patients improved significantly during the brief follow-up period. Patients who improved substantially with antipsychotic drug treatment had a significantly lower age and age at the onset of psychiatric illness. The patients who improved also had a significant reduction in total leucocyte count, absolute neutrophil count, and neutrophil-to-lymphocyte ratio from baseline to three-month follow-up. Duration of untreated illness had a significant positive correlation ( P = .042; r = 0.401) with the ESR. Conclusion: Inflammatory biomarkers are raised in drug naïve patients with ATPD, which improves with antipsychotic drug treatment, indicating a possible role of inflammation in the pathogenesis of ATPD.
Feasibility Study of a Culturally Tailored Support Group Program for the Psychosocial Wellbeing of Caregivers of Individuals Living With Dementia
Authors: Yiu Hing Cheung , Zang Yuli , Choi Kai Chow , Chau Janita Pak Chun 
Published: February 2025

Introduction: Culturally tailored support group interventions can help family caregivers. Little is known about the impacts of integrating philosophical thoughts in the interventions on the psychosocial wellbeing of family caregivers of persons with dementia. Methodology: A program integrating Chinese philosophies was provided to caregivers in the intervention group. A total of 33 and 30 caregivers in Hong Kong joined the intervention and control groups. They were asked to complete pre- and post-tests to assess their psychosocial health. Results: There were significant positive changes for the level of satisfaction with social support ( p < .05) and general quality of life ( p < .05). There were also significant improvements in the four aspects of coping (positive reframing: p < .01; planning: p < .05; humor: p < .01; religion: p < .01). Discussion: The intervention could provide insight into how culturally tailored interventions can improve the psychosocial wellbeing of family caregivers in Chinese communities.
Atrial Fibrillation Surgery in the Era of Minimally Invasive Surgery: Biatrial Versus Left Atrial Maze
Authors: Sharaf Omar M. , Murillo-Solera Alexandra , Beaver Thomas M. 
Published: February 2025

Objective: Atrial fibrillation management is rapidly evolving, particularly for patients who are intolerant to medical therapy. Several catheter-based, surgical, and hybrid approaches currently exist, each with unique benefits that may be harnessed to provide optimal outcomes for these patients. This review focuses on the use of a biatrial lesion set versus an isolated left atrial lesion set for ablation. Methods: Major representative articles for each ablation strategy were identified and included. Terms searched on PubMed, Google Scholar, and Scopus included “atrial fibrillation ablation,” “atrial fibrillation surgery,” and “maze procedure,” among others. Additional articles were included based on expert opinion. Results: The complete Cox maze biatrial lesion set has the highest efficacy but requires cardiopulmonary bypass. An isolated left atrial lesion set can also be performed, and these approaches are often less invasive but not as efficacious as the traditional complete maze operation. Conclusions: Although biatrial ablation may carry a higher risk of conduction abnormalities than isolated left atrial ablation in the setting of atrial fibrillation, biatrial ablation is more efficacious in maintaining sinus rhythm.
Fuchsite and other Cr-rich phyllosilicates in ultramafic enclaves from the Almadén mercury mining district, Spain
Authors: Morata D. , Higueras P. , Domínguez-Bella S. , Parras J. , Velasco F. , Aparicio P. 
Published: July 2002

AbstractFuchsite and other Cr-rich phyllosilicates, paragenetic with dolomite, are present in some ultramafic enclaves from the ‘frailesca’ rock (a lapilli- to block-size pyroclastic lithic-tuff), in the Almadén mercury mining district, Spain. Analyses (EMPA and TEM) of fuchsite and Cr-chlorite showed a relatively large range in levels of Cr2O3. Petrographic relationships between these phyllosilicates and primary relics of Cr-spinel crystals, as well as their high Cr content, indicate that these Cr-rich minerals originated from primary chromian spinels through an early hydrothermal alteration stage. The hydrothermal fluids accounting for this early alteration would be of relatively high temperature, high aCO2 and aK, and variable aNa/K. In a later alteration stage, fuchsite was partially or totally replaced by illite and Cr-illite, giving rise to an argillitic alteration.
Reactions of smectites with binuclear oxobridged iron complexes of N-alkyl-N,N-bis (2-pyridylmethyl)amines
Authors: Dick S. , Weiss A. 
Published: July 2002

AbstractCationic binuclear oxo- and carboxylate bridged Fe(III) complexes of N-alkyl-N,Nbis(2-pyridylmethyl)amines are introduced into the interlayer region of smectites by cation exchange reactions. The reaction can be divided into three steps. After a predominant loading of external surfaces, cation exchange sites at internal surfaces react, accompanied by considerable stmctaral expansion. Both reaction stages belong to the steep section of the exchange isotherms. At a certain loading, which depends on the complex substituents and the layer charge of the smectite, the isotherms become flat. With bulky shaped complex cations and highly charged smectites, exchange is not quantitative; vermiculite-type minerals show a very small complex uptake. For this behaviour, both thermodynamic and kinetic reasons must be considered. From these results the number of cation exchange positions at external surfaces of smectite crystals may be estimated.
Apparent pyrrhotite-chalcopyrite solid solutions in charnockites: the Shevaroy Hills Massif, Tamil Nadu, S India and the Bamble Sector, SE Norway
Authors: Harlov D. E. 
Published: July 2002

AbstractExamples of apparent exsolution lamellae and lenticular blebs of chalcopyrite in pyrrhotite are described in orthopyroxene-bearing granulite facies rocks from two, oxidized (log10fO2= −14 to −11), widely separated, well characterized high grade terranes: the Bamble Sector, SE Norway (795°C, 7.5 kbar) and the Shevaroy Hills Massif, Tamil Nadu, S India (750°C, 7.5 kbar). These exsolution features only occur in isolated pyrrhotite grains and not in integral pyrrhotite-pyrite-chalcopyrite-magnetite grain clusters which essentially represent an oxidation equilibrium. Reintegration of these chalcopyrite exsolution features back into the pyrrhotite host indicate Cu contents ranging from 1 to 5 wt.% in good agreement with experimental observations which indicate that pyrrhotite can take up to 7 wt.% Cu at temperatures above 800°C at pressures of ∼1 bar. This suggests that under high grade conditions these chalcopyrite exsolution features were in solid solution with pyrrhotite. Whether Cu stabilizes pyrrhotite at higher oxygen fugacities or these chalcopyrite-pyrrhotite grains represent a metastable phase is uncertain. One possibility is that the isolated pyrrhotite grains with chalcopyrite lamellae could represent grains that were preferentially not exposed to infiltrating fluids, which oxidized the pyrrhotites in other areas of the sample. A second possibility is that either these grains had enough Cu to stabilize them during pervasive infiltration of oxidizing fluids or that they represent a metastable phase with respect to the overall oxygen fugacity of the sample. The two conclusions that can be drawn from these observations are, firstly, that it is possible for pyrrhotite and chalcopyrite to form a limited solid solution at granulite facies temperatures and pressures under relatively high oxidizing conditions, i.e. 1.5 log units above fayalite-magnetite-quartz, at 800°C and 8 kbar. Secondly, this limited solid solution should have some bearing on the stability of pyrrhotite with respect to co-existing magnetite and pyrite as a function of the oxidation state of the rock, be it inherited or fluid induced.
CrkL is an adapter for Wiskott-Aldrich syndrome protein and Syk
Authors: Oda Atsushi , Ochs Hans D. , Lasky Laurence A. , Spencer Susan , Ozaki Katsutoshi , Fujihara Mitsuhiro , Handa Makoto , Ikebuchi Kenji , Ikeda Hisami 
Published: July 2002

AbstractWiskott-Aldrich syndrome (WAS) and X-linked thrombocytopenia are caused by mutations of the WAS protein (WASP) gene. WASP may be involved in the regulation of podosome, an actin-rich dynamic cell adhesion structure formed by various types of cells. The molecular links between WASP and podosomes or other cell adhesion structures are unknown. Platelets express an SH2-SH3 adapter molecule, CrkL, that can directly associate with paxillin, which is localized in podosomes. The hypothesis that CrkL binds to WASP was, therefore, tested. Results from coprecipitation experiments using anti-CrkL and GST-fusion proteins suggest that CrkL binds to WASP through its SH3 domain and that the binding was not affected by WASP tyrosine phosphorylation. The binding of GST-fusion SH3 domain of PSTPIP1 in vitro was also not affected by WASP tyrosine phosphorylation, suggesting that the binding of the SH3 domains to WASP is not inhibited by tyrosine phosphorylation of WASP. Anti-CrkL also coprecipitates a 72-kd protein, which was identified as syk tyrosine kinase, critical for collagen induced-platelet activation. CrkL immunoprecipitates contain kinase-active syk, as evidenced by an in vitro kinase assay. Coprecipitation experiments using GST-fusion CrkL proteins suggest that both SH2 and SH3 domains of CrkL are involved in the binding of CrkL to syk. WASP, CrkL, syk, and paxillin-like Hic-5 incorporated to platelet cytoskeleton after platelet aggregation. Thus, CrkL is a novel molecular adapter for WASP and syk and may potentially transfer these molecules to the cytoskeleton through association with cytoskeletal proteins such as Hic-5.
Reconstitution of bactericidal activity in chronic granulomatous disease cells by glucose-oxidase–containing liposomes
Authors: Gerber Claudia E. , Bruchelt Gernot , Falk Ulrike B. , Kimpfler Andrea , Hauschild Oliver , Kuçi Selim , Bächi Thomas , Niethammer Dietrich , Schubert Rolf 
Published: July 2002

AbstractChronic granulomatous disease (CGD) is an inherited primary immunodeficiency characterized by phagocytes devoid of a functioning nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The failure of CGD phagocytes to produce reactive oxygen species (ROS) results in a marked increase in the susceptibility of affected patients to life-threatening bacterial and fungal infections. This study investigated whether loading of CGD phagocytes with glucose oxidase (GO)–containing liposomes (GOLs) could restore cellular production of bactericidal ROS (eg, H2O2 and HOCl) in vitro. Results indicate that GO encapsulated in liposomes enabled NADPH oxidase-deficient phagocytes to use H2O2 for the production of highly bactericidal HOCl. The intracellular colocalization of bacteria and liposomes (or liposome-derived ferritin) was demonstrated by confocal laser microscopy and electron microscopy. After uptake of GOLs (approximately 0.2 U/mL at 1 mM total lipid concentration, size approximately 180 nm), CGD granulocytes produced HOCl levels comparable to those of normal phagocytes. Remarkably, after treatment with GOLs, CGD phagocytes killed Staphylococcus aureus as efficiently as normal granulocytes. Moreover, treated cells retained sufficient motility toward chemotactic stimuli as measured by chemotaxis assay. Side effects were evaluated by measuring the H2O2 concentrations and the production of methemoglobin in whole blood. These studies revealed that H2O2 produced by GOLs was degraded immediately by the antioxidative capacity of whole blood. Elevated methemoglobin levels were observed only after application of extremely high amounts of GOLs (2 U/mL). In summary, the application of negatively charged GOLs might provide a novel effective approach in the treatment of patients with CGD at high risk for life-threatening infections.
Decreased immune functions of blood cells following mobilization with granulocyte colony-stimulating factor: association with donor characteristics
Authors: Joshi Shantaram S. , Lynch James C. , Pavletic Steve Z. , Tarantolo Stefano R. , Pirruccello Samuel J. , Kessinger Anne , Bishop Michael R. 
Published: July 2002

AbstractIn this study, mononuclear cells (MNCs) from granulocyte colony-stimulating factor (G-CSF)–mobilized blood stem cell (BSC) harvests from 104 healthy donors were analyzed for their immunological functions and compared with MNCs from 28 steady-state nonmobilized donors. The relationships between donor characteristics (age, gender, weight, and HLA type) and immune functions of the harvests were also analyzed. There was a significant (P &lt; .01) decrease in natural killer and lymphokine-activated killer (LAK) cell–mediated cytotoxicity for G-CSF–mobilized effector cells compared with nonmobilized cells. Similarly, there was a significant (P &lt; .005) decrease in both T-cell and B-cell mitogen response in G-CSF–mobilized cells compared with nonmobilized cells. There was dose-dependent inhibition of LAK cell–mediated cytotoxicity, but this effect was not seen with other immune function assays. Changes in immune function did not appear to be determined by frequency of cellular phenotypes or expression of effector function genes seen in a reverse-transcription polymerase chain reaction. There was a significant relationship between expression of certain HLA alleles (A1, A3, A24, B44, B62, DR15, DR17; allP &lt; .01) and increased immune function, such as cytotoxicity and/or mitogen response. A decrease in immune function with the HLA-DR13 expression was also observed (P &lt; .01). Since the G-CSF increases the number of MNCs, the increase in effector cells might compensate for decreased immune functions of these cells in vivo when transplanted into patients. These results suggest a decreased immune function in G-CSF–mobilized BSC harvests and warrant further studies to correlate these data with clinical outcome.
Complete genome assemblies and antibiograms of 22 Staphylococcus capitis isolates
Authors: Wan Yu , Pike Rachel , Harley Alessandra , Mumin Zaynab , Potterill Isabelle , Meunier Danièle , Ganner Mark , Getino Maria , Coelho Juliana , Jauneikaite Elita , Moganeradj Kartyk , Brown Colin S. , Holmes Alison H. , Demirjian Alicia , Hopkins Katie L. , Pichon Bruno 
Published: February 2025

Abstract

Objective

Staphylococcus capitis is part of the human microbiome and an opportunistic pathogen known to cause catheter-associated bacteraemia, prosthetic joint infections, skin and wound infections, among others. Detection of S. capitis in normally sterile body sites saw an increase over the last decade in England, where a multidrug-resistant clone, NRCS-A, was widely identified in blood samples from infants in neonatal intensive care units. To address a lack of complete genomes and antibiograms of S. capitis in public databases, we performed long- and short-read whole-genome sequencing, hybrid genome assembly, and antimicrobial susceptibility testing of 22 diverse isolates.

Data description
We present complete genome assemblies of two S. capitis type strains (subspecies capitis: DSM 20326; subspecies urealyticus: DSM 6717) and 20 clinical isolates (NRCS-A: 10) from England. Each genome is accompanied by minimum inhibitory concentrations of 13 antimicrobials including vancomycin, teicoplanin, daptomycin, linezolid, and clindamycin. These 22 genomes were 2.4–2.7 Mbp in length and had a GC content of 33%. Plasmids were identified in 20 isolates. Resistance to teicoplanin, daptomycin, gentamicin, fusidic acid, rifampicin, ciprofloxacin, clindamycin, and erythromycin was seen in 1–10 isolates. Our data are a resource for future studies on genomics, evolution, and antimicrobial resistance of S. capitis.
Prevalence and characterization of class I integrons in multidrug-resistant Escherichia coli isolates from humans and food-producing animals in Zhejiang Province, China
Authors: Jiang Han , Ran Meijuan , Wang Xinyuan , Chen Qi , Wang Jing , Ruan Zhi , Wang Jingwen , Tang Biao , Fang Jiehong 
Published: February 2025

Abstract
Class I integrons have garnered significant attention due to pivotal roles in the dissemination of antimicrobial resistance genes (ARGs), which impose risks to public health and food safety. Here, the prevalence and characteristics of class I integrons in Escherichia coli isolates derived from food-producing animals and human patients were assessed. Of 721 E. coli isolates collected from human patients (113), pigs (298), and poultry (310), 93 (12.90%) carried the class I integrase gene (intI1). Multilocus sequence typing identified 39 sequence types from 93 intI1-postive isolates, including three novel types. Sequence analysis revealed that 59 classical class I integrons encompassed six distinct gene cassettes arrangements [dfrA17-aadA5, dfrA12-aadA2, dfrA1-aadA1, dfrA7, aac(6’)-Ib, and aadA1-aac(3)-VIa]. Six insertion sequences (IS1, IS6, IS21, IS91, IS110, and IS256) and one transposon (Tn3) were harbored in proximity to the integrons. A comparison with sequences retrieved from the National Center for Biotechnology Information database demonstrated that E. coli isolates with integron sequences were detected in various food-producing animals and human hosts in environmental niches across Asia, Europe, and North America. These findings indicate the potential risk of ARG transmission between food-producing animals and humans by bacteria populations and provide useful baseline data for monitoring of ARGs.
Assessing the quality and integrating the evidence and strength of recommendations in the guidelines for gastric precancerous lesions
Authors: Ou Jia-yin , Liu Yang , Zhang Lang , Luo Tian-qi , Li Jia-yu , Lu Li-ming , Wang Lin , He Qiu-rong , Liu Xin , Pan Hua-feng 
Published: February 2025

Abstract

Background
Clinical practice guidelines (CPGs) are intended to offer appropriate recommendations for clinical practice based on the available evidence while acknowledging existing gaps and uncertainties. The quality of CPGs for gastric precancerous lesions (GPL), distribution of evidence quality, and strength of recommendations are unknown.

Objective
Systematically evaluate the quality of CPGs for GPL and identify areas for improvement in the development process.

Methods
PubMed, Embase, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, and six online CPG repositories were systematically searched for CPGs related to GPL. Three researchers independently assessed the methodological quality of the included CPGs by using the AGREE II tool. The reporting and recommendation quality of the CPGs were evaluated using the RIGHT and AGREE-REX tools through consensus. Evidence-based CPGs were analyzed using the Grading of Recommendation Assessment, Development, and Evaluation system to determine the distribution of quality of evidence and strength of recommendations.

Results
A total of 4046 records were identified; nine CPGs met the eligibility criteria for this study. The mean overall score for the methodological quality of the CPGs was 46.22%. Among the six domains, the mean score for clarity of presentation was the highest (71.67%), while the mean score for applicability was the lowest (24.56%). Among the nine CPGs, only one was considered high quality. Regarding reporting quality, domains 1, 3, and 4 had mean reporting rates equal to or higher than 60%. The mean overall score for the recommendation quality was 19.11%. In total, 235 recommendations were identified through the screening process, of which 64.4% were classified as strong. However, only 17.5% of the strong recommendations were supported by high-quality evidence.

Conclusion
The overall quality of CPGs for GPL was poor, with uneven quality across domains. In addition, the consistency between the strength of recommendations and the quality of evidence was poor.
Clinical internship experience of African undergraduate nursing students in China: a descriptive qualitative study
Authors: Chen Lingxi , Xu Chao , Zhou Yunxian 
Published: February 2025

Abstract

Background
Clinical internships are crucial in nursing education; however, they often present challenges that can elicit negative emotions among students, especially among international nursing undergraduates. Despite its importance, there is a lack of research on the experiences of African nursing undergraduates during clinical internships in China.

Aim
To explore the clinical internship experiences of African undergraduate nursing students within the Chinese healthcare system.

Method
A descriptive qualitative study design was adopted. Thirteen African undergraduate nursing students with over three months of clinical internship experience in China were recruited through purposive sampling. Semi-structured interviews were conducted to collect data on their experiences. Conventional content analysis was used for data analysis.

Results
Five categories and twelve subcategories were identified through the analysis: multiple challenges (language is an issue, identity embarrassment, peer pressure); accepting challenges (having to accept, being able to accept); making personal efforts (self-image managing, self-reflecting and improving); being not alone (preceptors are strong backings, mutual assistance among fellow students, patients provide trust and warmth); and growing up as a real nurse (increasing nursing knowledge and skills, enhancing nursing professional quality).

Conclusions
The findings offer nursing colleges and hospitals a deeper understanding of the specific challenges encountered by African undergraduate nursing students during their clinical internships in China. This study also highlights the students’ handling of complex issues and the subsequent positive changes they experienced.