bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrafast spontaneous emission source using plasmonic nanoantennas
Authors: Hoang Thang B. , Akselrod Gleb M. , Argyropoulos Christos , Huang Jiani , Smith David R. , Mikkelsen Maiken H. 
Published: July 2015

AbstractTypical emitters such as molecules, quantum dots and semiconductor quantum wells have slow spontaneous emission with lifetimes of 1–10 ns, creating a mismatch with high-speed nanoscale optoelectronic devices such as light-emitting diodes, single-photon sources and lasers. Here we experimentally demonstrate an ultrafast (<11 ps) yet efficient source of spontaneous emission, corresponding to an emission rate exceeding 90 GHz, using a hybrid structure of single plasmonic nanopatch antennas coupled to colloidal quantum dots. The antennas consist of silver nanocubes coupled to a gold film separated by a thin polymer spacer layer and colloidal core–shell quantum dots, a stable and technologically relevant emitter. We show an increase in the spontaneous emission rate of a factor of 880 and simultaneously a 2,300-fold enhancement in the total fluorescence intensity, which indicates a high radiative quantum efficiency of ∼50%. The nanopatch antenna geometry can be tuned from the visible to the near infrared, providing a promising approach for nanophotonics based on ultrafast spontaneous emission.
Parallel damage in mitochondrial and lysosomal compartments promotes efficient cell death with autophagy: The case of the pentacyclic triterpenoids
Authors: Martins Waleska K. , Costa Érico T. , Cruz Mário C. , Stolf Beatriz S. , Miotto Ronei , Cordeiro Rodrigo M. , Baptista Maurício S. 
Published: July 2015

AbstractThe role of autophagy in cell death is still controversial and a lot of debate has concerned the transition from its pro-survival to its pro-death roles. The similar structure of the triterpenoids Betulinic (BA) and Oleanolic (OA) acids allowed us to prove that this transition involves parallel damage in mitochondria and lysosome. After treating immortalized human skin keratinocytes (HaCaT) with either BA or OA, we evaluated cell viability, proliferation and mechanism of cell death, function and morphology of mitochondria and lysosomes and the status of the autophagy flux. We also quantified the interactions of BA and OA with membrane mimics, bothin-vitroand in-silico. Essentially, OA caused mitochondrial damage that relied on autophagy to rescue cellular homeostasis, which failed upon lysosomal inhibition by Chloroquine or Bafilomycin-A1. BA caused parallel damage on mitochondria and lysosome, turning autophagy into a destructive process. The higher cytotoxicity of BA correlated with its stronger efficiency in damaging membrane mimics. Based on these findings, we underlined the concept that autophagy will turn into a destructive outcome when there is parallel damage in mitochondrial and lysosomal membranes. We trust that this concept will help the development of new drugs against aggressive cancers.
Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum
Authors: Chen Yun , Gao Qixun , Huang Mengmeng , Liu Ye , Liu Zunyong , Liu Xin , Ma Zhonghua 
Published: July 2015

AbstractThe RNA interference (RNAi) plays a critical role in gene regulation in a variety of eukaryotic organisms. However, the role of RNAi remains largely unclear in plant pathogenic fungi. In this study, we explored the roles of core components of the RNAi pathway in Fusarium graminearum, the major causal agent of wheat head blight. Our results demonstrated that the hairpin RNA (hpRNA) can efficiently silence the expression level of target gene and the argonaute protein FgAgo1 and dicer protein FgDicer2 are important in this silencing process. RNAi machinery was not involved in growth, abiotic stress and pathogenesis in F. graminearum under tested conditions. We firstly applied high-throughput sequencing technology to elucidate small RNA (17–40 nucleotides) (sRNA) transcriptome in F. graminearum and found that a total of forty-nine micro-like-RNA (milRNA) candidates were identified in the wild-type and ∆FgDICER2 and twenty-four of them were FgDicer2-dependent. Fg-milRNA-4 negatively regulated expression of its target gene. Taken together, our results indicated that the hpRNA-induced gene silencing was a valuable genetic tool for exploring gene function in F. graminearum. FgAgo1 and FgDicer2 proteins played a critical role in the hpRNA mediated gene silencing process. In addition, FgDicer2 was involved in sRNA transcription and milRNA generation in this fungus.
Basolateral amygdala rapid glutamate release encodes an outcome-specific representation vital for reward-predictive cues to selectively invigorate reward-seeking actions
Authors: Malvaez Melissa , Greenfield Venuz Y. , Wang Alice S. , Yorita Allison M. , Feng Lili , Linker Kay E. , Monbouquette Harold G. , Wassum Kate M. 
Published: July 2015

AbstractEnvironmental stimuli have the ability to generate specific representations of the rewards they predict and in so doing alter the selection and performance of reward-seeking actions. The basolateral amygdala participates in this process, but precisely how is unknown. To rectify this, we monitored, in near-real time, basolateral amygdala glutamate concentration changes during a test of the ability of reward-predictive cues to influence reward-seeking actions (Pavlovian-instrumental transfer). Glutamate concentration was found to be transiently elevated around instrumental reward seeking. During the Pavlovian-instrumental transfer test these glutamate transients were time-locked to and correlated with only those actions invigorated by outcome-specific motivational information provided by the reward-predictive stimulus (i.e., actions earning the same specific outcome as predicted by the presented CS). In addition, basolateral amygdala AMPA, but not NMDA glutamate receptor inactivation abolished the selective excitatory influence of reward-predictive cues over reward seeking. These data support the hypothesis that transient glutamate release in the BLA can encode the outcome-specific motivational information provided by reward-predictive stimuli
An in silico platform for predicting, screening and designing of antihypertensive peptides
Authors: Kumar Ravi , Chaudhary Kumardeep , Singh Chauhan Jagat , Nagpal Gandharva , Kumar Rahul , Sharma Minakshi , Raghava Gajendra P.S. 
Published: July 2015

AbstractHigh blood pressure or hypertension is an affliction that threatens millions of lives worldwide. Peptides from natural origin have been shown recently to be highly effective in lowering blood pressure. In the present study, we have framed a platform for predicting and designing novel antihypertensive peptides. Due to a large variation found in the length of antihypertensive peptides, we divided these peptides into four categories (i) Tiny peptides, (ii) small peptides, (iii) medium peptides and (iv) large peptides. First, we developed SVM based regression models for tiny peptides using chemical descriptors and achieved maximum correlation of 0.701 and 0.543 for dipeptides and tripeptides, respectively. Second, classification models were developed for small peptides and achieved maximum accuracy of 76.67%, 72.04% and 77.39% for tetrapeptide, pentapeptide and hexapeptides, respectively. Third, we have developed a model for medium peptides using amino acid composition and achieved maximum accuracy of 82.61%. Finally, we have developed a model for large peptides using amino acid composition and achieved maximum accuracy of 84.21%. Based on the above study, a web-based platform has been developed for locating antihypertensive peptides in a protein, screening of peptides and designing of antihypertensive peptides.
Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis
Authors: Mkrtchyan Garik , Aleshin Vasily , Parkhomenko Yulia , Kaehne Thilo , Luigi Di Salvo Martino , Parroni Alessia , Contestabile Roberto , Vovk Andrey , Bettendorff Lucien , Bunik Victoria 
Published: July 2015

AbstractThiamin (vitamin B1) is a pharmacological agent boosting central metabolism through the action of the coenzyme thiamin diphosphate (ThDP). However, positive effects, including improved cognition, of high thiamin doses in neurodegeneration may be observed without increased ThDP or ThDP-dependent enzymes in brain. Here, we determine protein partners and metabolic pathways where thiamin acts beyond its coenzyme role. Malate dehydrogenase, glutamate dehydrogenase and pyridoxal kinase were identified as abundant proteins binding to thiamin- or thiazolium-modified sorbents. Kinetic studies, supported by structural analysis, revealed allosteric regulation of these proteins by thiamin and/or its derivatives. Thiamin triphosphate and adenylated thiamin triphosphate activate glutamate dehydrogenase. Thiamin and ThDP regulate malate dehydrogenase isoforms and pyridoxal kinase. Thiamin regulation of enzymes related to malate-aspartate shuttle may impact on malate/citrate exchange, responsible for exporting acetyl residues from mitochondria. Indeed, bioinformatic analyses found an association between thiamin- and thiazolium-binding proteins and the term acetylation. Our interdisciplinary study shows that thiamin is not only a coenzyme for acetyl-CoA production, but also an allosteric regulator of acetyl-CoA metabolism including regulatory acetylation of proteins and acetylcholine biosynthesis. Moreover, thiamin action in neurodegeneration may also involve neurodegeneration-related 14-3-3, DJ-1 and β-amyloid precursor proteins identified among the thiamin- and/or thiazolium-binding proteins.
Doubling Throughput of a Real-Time PCR
Authors: Ahrberg Christian D. , Neužil Pavel 
Published: July 2015

AbstractThe invention of polymerase chain reaction (PCR) in 1983 revolutionized many areas of science, due to its ability to multiply a number of copies of DNA sequences (known as amplicons). Here we report on a method to double the throughput of quantitative PCR which could be especially useful for PCR-based mass screening. We concurrently amplified two target genes using only single fluorescent dye. A FAM probe labelled olionucleotide was attached to a quencher for one amplicon while the second one was without a probe. The PCR was performed in the presence of the intercalating dye SYBR Green I. We collected the fluorescence amplitude at two points per PCR cycle, at the denaturation and extension steps. The signal at denaturation is related only to the amplicon with the FAM probe while the amplitude at the extension contained information from both amplicons. We thus detected two genes within the same well using a single fluorescent channel. Any commercial real-time PCR systems can use this method doubling the number of detected genes. The method can be used for absolute quantification of DNA using a known concentration of housekeeping gene at one fluorescent channel.
The discursive (de)legitimisation of global governance: political contestation and the emergence of new actors in the WTO’s Dispute Settlement Body
Authors: Michael Strange 
Published: July 2015

The World Trade Organization's Dispute Settlement Body provides the teeth of the global trade regime – empowering it with substantial means to adjudicate in disagreements between Memberstates over the implementation of WTO law. The WTO's teeth have, however, also helped make the organisation controversial. The Dispute Settlement Body has frequently found itself at the centre of a much wider societal critique of the broader WTO – as well as contemporary global trade governance – in which its legitimacy to operate has been fiercely questioned.
The political sensitivity of its work has been made most apparent in those cases where the principles of WTO law appear to run counter to environmental or consumer safety concerns, taking the system into the mass media and making it the subject of street protests. Yet, where rulings have given new access to non-state actors campaigning for these concerns (e.g. amicus curiae provisions), there has been further controversy amongst Member-states over whether the Dispute Settlement Body has acted outside its delegated authority by effectively rewriting ‘who’ or ‘what’ is an actor in the system.
The changing character of this specific institutional arrangement is approached in the article as part of a wider struggle over the terms of what is ‘legitimate’ in global governance. Where WTO Dispute Settlement has been re-politicised, both inside and out-side the formal institution, a contradiction becomes visible – between its legal-technocratic identity and a world that is fundamentally political. The legal normalisation of new actor identities needs to be understood in this context, as an attempt to manage that tension and reinforce the claim that WTO Dispute Settlement is legitimate. How the institution has changed and new identities emerged since its birth in 1995 is enhanced if understood in the context of a struggle in which the terms of what is legitimate in global governance are ultimately unfixed.
Attitudes of undergraduate business students toward sustainability issues
Authors: Eagle Lynne , Low David , Case Peter , Vandommele Lisa 
Published: July 2015

Purpose– This paper aims to report on findings from the first phase of a longitudinal study of undergraduate business students’ attitudes, beliefs and perceptions concerning sustainability issues.Design/methodology/approach– To improve understanding of the potential effects of changes in the curriculum, business students enrolled during the academic year prior to a redesigned, sustainability-informed, curriculum were surveyed. Familiarity with key sustainability terms was tested using a semi-structured questionnaire applied across two campuses of James Cook University, Australia. Quantitative data were complemented by use of open-ended questions that yielded qualitative insight into a range of student knowledge, attitudes, behaviours and normative influences relating to sustainability and climate change.Findings– Findings reflect naïve awareness of the potential impact of individual contributions to sustainability and environmental challenges. They reveal a tendency to regard major issues as beyond personal control and to view solutions as being the responsibility of others. This is coupled with reluctance to consider major lifestyle changes.Social implications– Universities are increasing their focus on sustainability-related issues and the ways in which these can be effectively communicated via curricula. This paper carries implications for this societal agenda, particularly in relation to the need to address disconnections between awareness of issues, personal relevance and effective strategies for addressing sustainability issues.Originality/value– The findings shed fresh light on the attitudes and behavioural dispositions of undergraduate business students and could help guide the development and delivery of curriculum content.
Participatory processes in sustainable universities – what to assess?
Authors: Disterheft Antje , Azeiteiro Ulisses M , Leal Filho Walter , Caeiro Sandra 
Published: July 2015

Purpose
– This paper aims to connect participatory sustainability implementation with sustainability assessment, exploring learning theories, the principles of Higher Education for Sustainable Development (HESD) and respective indicators applied in the university context. Even though participation is partly considered in existing assessment practices, it is still unclear what and how to measure participatory processes that envision implementing sustainability principles in higher education institutions. Holistic approaches are often proclaimed, but reductionist assessment methods are frequently followed.

Design/methodology/approach
– The study followed a qualitative approach, inspired by the Delphi method, and includes semi-structured expert interviews (N = 15) and two focus group discussions (N = 23), with participants coming from a total of 17 different countries. Data were analysed and compared according to qualitative content analysis and systemized according to the underlying theoretical strands.

Findings
– The findings suggest that participatory processes can be better assessed from a social learning and organisational learning perspective, emphasizing non-linear criteria for the quality of the process in terms of depth and meaningfulness as well as criteria for the quality of the outcome in terms of knowledge generation and innovation. The findings also point implicitly to the need of considering double- and triple-loop learning, if a culture of participation towards sustainability is to be pursued, and underline the high impact of institutional governance.

Originality/value
– Although a great volume of literature about sustainability implementation in higher education exists, studies focusing on participatory processes in this context are rather scarce. This research pays attention to sustainability experts working in universities rarely heard in a more systemic manner and also applies a reflective participatory approach itself by using qualitative methods.
Experiential learning for sustainability leadership in higher education
Authors: Savage Emma , Tapics Tara , Evarts John , Wilson Jeffrey , Tirone Susan 
Published: July 2015

Purpose
– The purpose of this paper is to compare the program design of a sustainability leadership certificate to participants’ perceptions of their in-program learnings and competencies development. The authors present the results from the analysis of one program evaluation component, a survey, which was delivered before the program start and at the program end.

Design/methodology/approach
– The authors describe key design elements of a sustainability leadership certificate, which was framed around five key sustainability competencies. Using a pre/post self-assessment, participants (n = 32) selected their level of confidence and competence in each of the key sustainability competencies and completed open-ended questionnaires. Quantitative data were analyzed using a Mann–Whitney U test, and qualitative data were transcribed and coded using a grounded theory approach in NVivo 10.

Findings
– Based on the survey feedback, the program participants were generally excited by the program’s experiential format and supportive community. They felt that they had improved their confidence and competence in the key sustainability competencies. Three themed clusters, community, future and personal development, emerged from the participants’ open-ended responses. This supports the program design and can inform further program development.

Practical implications
– The third theme, personal development, is notable, as it is not a typical focus of sustainability in higher education, but held high importance to participants. This strong resonance with participants suggests that sustainability programs should consider the role of the self to foster the development of key sustainability competencies.

Originality/value
– The program’s focus on “personal” was intentional in the program design. Based on participants’ feedback, the inclusion of personal development exercises was a critical element for successful sustainability leadership development.
Teams as technology: strengths, weaknesses, and trade-offs in cognitive task performance
Authors: Hinsz Verlin 
Published: July 2015

Purpose
– This paper aims to assert that teams are a technology used to achieve task goals or social objectives that cannot be accomplished by individuals alone. Much current work in organizations is knowledge based, so it is important to know when to apply teams as a technology and how teams can be effectively utilized for cognitive task performance. This paper describes a number of strengths, weaknesses and trade-offs that accompany teams performing cognitive tasks.

Design/methodology/approach
– Research comparing team performance to that of similarly treated individuals indicates that teams on average exceed the performance of individuals on cognitive tasks; however, teams rarely match the performance of their best member.

Findings
– Based on analysis of this research, a set of strengths of teams are highlighted: information pooling, error correction, meta-knowledge, reliability and information sharing. Two weaknesses of team performance on cognitive tasks are also identified: slow to action and coordination losses. As a function of teams having these strengths and weaknesses, trade-offs in their task performance emerge: speed versus accuracy, convergence versus divergence, participation versus deindividuation, losses versus gains in motivation, social facilitation versus inhibition, accumulation versus coordination, focused versus distributed attention and accentuation versus attenuation of biases.

Originality/value
– These trade-offs demonstrate that teams operate in specific ways that sometimes benefit cognitive processing but will be hindered under other conditions. An understanding of those conditions is important when attempting to effectively use teams. So, “technical” knowledge rather than intuition is required to manage these processes appropriately and effectively.
Caesarean section rates in subgroups of women and perinatal outcomes
Authors: Zhang J , Geerts C , Hukkelhoven C , Offerhaus P , Zwart J , de Jonge A 
Published: July 2015

ObjectivesTo identify factors that are associated with a relatively low caesarean section (CS) rate by examining the CS rate in various subgroups in the Netherlands.DesignCross‐sectional analysis.Settingsthe Netherlands.PopulationA total of 685 452 births in the Netherlands Perinatal Registry from 2007 to 2010.MethodsA modified classification system for CS was used to categorise all women into ten groups. Labour management, mode of delivery, maternal and neonatal morbidity and mortality were assessed according to these ten groups.Main outcome measuresCaesarean section, labour induction, instrumental delivery, postpartum haemorrhage, perineal laceration, duration of second stage of labour, Apgar score, fetal and neonatal mortality.ResultsTotal CS rate was 15.6%. Term, nulliparous and parous women with a singleton pregnancy of a fetus in cephalic position and spontaneous onset of labour had CS rates of 9.6 and 1.9% and instrumental birth rates of 19.4 and 2.4%, respectively; 17.3% of births were induced. Among women with a previous CS and term, singleton pregnancies with a fetus in cephalic presentation, 71% had trial of labour, of which 75% had a successful vaginal birth. Of women with multiple gestation, 43% had CS. Women with CS due to ‘failure to progress’ in the second stage of labour had a median duration of second‐stage pushing of almost 2 hours in nulliparas and >90 minutes in parous women.ConclusionsSeveral obstetric practice patterns may have contributed to the relatively low overall CS rate in the Netherlands: a relatively low CS rate in term, singleton pregnancies of a fetus in cephalic position and spontaneous onset of labour, relatively low rate of labour induction, a high rate of a trial of labour after a previous CS, the use of vacuum and forceps, and a high proportion of women being taken care of by midwives.Tweetable abstractThe Netherlands has several practice patterns that may have contributed to its relatively low CS rate.