bluuebunny/crossref_metadata_2025_split huggingface dataset:
ABNORMAL AND UNSTABLE PATTERNS OF THE DNA OF STROMA CELLS IN A PATIENT WITH TUBEROUS SCLEROSIS
Authors: Ishibashi Yasumasa , Inoue Yukiko , Takehara Kazuhiko , Kukita Atsushi , Murakami Yasufumi , Hanaoka Fumio , Yamada Masa‐Atsu 
Published: April 2015

ABSTRACTExplant cultures were prepared using normal‐looking skin and the so‐called adenoma sebaceum (AS) from the face of a 42‐year‐old male with tuberous sclerosis, Pringle's disease (PD), and the DNA content of the subcultured non‐epithelial cells (NECs), stroma cells, were investigated per nucleus and per cell by a flow cytofluorometer.

The DNA histogram per nucleus of the NECs from the normal‐looking skin of the patient did not reveal any remarkable differences from that of the normal control group.
In contrast, the DNA histograms per nucleus of cells from AS in the patient showed distinct and different patterns in comparison with those of the normal control group, including an increase in width and decrease in height of 2C peak, vanishing of 4C peak, and formation of a new small peak at the left end of the histogram.
On the other hand, the DNA histogram per cell of the NECs from the normal‐looking skin of the patient displayed a slightly different pattern from that per nucleus, with a distinct increase in width and a faint indentation.
Furthermore, the DNA histograms per cell of the NECs from AS exhibited drastic changes such as remarkable flattening and increase in the width of the 2C peak with a newly formed small peak at the left end of this histogram. However, these changes also varied according to the population density of large dendritic cells in the culture dish, even though these NECs were derived from the same AS tissue.
The above‐mentioned findings suggest that the amount of DNA per nucleus and probably per cell might not be equal among atypical NECs from AS in the patient even in the same G0•G1 phase.
PROMOTION OF COAGULASE RELEASE BY SERUM ALBUMIN IN SAPHYLOCOCCUS AUREUS DERIVED FROM FURUNCLE
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

ABSTRACTThree strains of Staphylococcus aureus derived from furuncles were cultured in media with and without bovine serum albumin, and coagulase titers in the media were examined. Although the titers were increased during the early to mid log phases of growth and decreased after the mid log phase in the medium without serum albumin, they were increased both during the early to mid log phase, and after the mid log phase as well in the medium with serum albumin. As coagulase probably protects the bacteria from the natural defense mechanisms of the host, these results suggest that Staphylococcus aureus introduced to damaged skin surfaces with accumulated plasma‐like fluid proliferates well because of the prolonged high coagulase level around them, allowing the number of bacteria to become great enough to produce a furuncle.
HETEROGENEITY OF EPIDERMAL PREKERATIN AND KERATIN BETWEEN SOLE AND BODY SKIN IN HUMANS
Authors: Kitajima Yasuo , Furuta Hiroko , Tsuneda Yoriko , Yoneda Kazufumi , Mori Shunji 
Published: April 2015

ABSTRACTRecently, heterogeneity of stratum corneum keratin (α‐fibrous protein) in various anatomic sites has been described in humans as well as cows and rats. In order to study this heterogeneity more clearly in humans, we compared acid soluble (citrate buffer at pH 2.65) prekeratin, acid insoluble (8 M urea‐2‐mercaptoethanol soluble) living cell keratin, and stratum corneum keratin from sole and body (nonsole‐nonpalm) skin using SDS‐polyacrylamide gel electrophoresis (PAGE), amino acid analysis, and immunological analyses.In prekeratin, no differences were seen between sole and body by SDS‐PAGE. Prekeratins from the two sources showed a 4‐band pattern with molecular weights of 55,000, 59,000, 62,000 and 69,000. In living cell keratin, the 69,000 band disappeared and a 49,000 band appeared in sole, but not in body epidermis. In stratum corneum keratin, a distinct difference was seen between sole and body skin. The sole stratum corneum keratin showed a 3‐band pattern (55,000, 59,000 and 62,000 mw) while that of body showed a two‐band pattern (59,000 and 66,000 mw).Despite the heterogeneity of the SDS‐PAGE profiles, no significant differences were seen in amino acid composition amongst these specimens.Immunologically, using double diffusion, antiserum against stratum corneum keratin from the sole principally produced two precipitin lines against the same keratin of sole origin and one precipitin line against prekeratin, living cell keratin, and stratum corneum keratin of body origin. This precipitin line fused with one of the two precipitin lines produced between antiserum and the sole stratum corneum keratin.These results indicate that anatomical‐site heterogeneity in α‐fibrous protein of human epidermis does not exist at the acid‐soluble prekeratin level, but does at the acid‐insoluble keratin level of living cell layer and stratum corneum when examined by SDS‐PAGE, and that some, but not all, keratin antigens are immunologically common between sole and body.
DISTURBED MITOTIC PROCESSES OF STROMA CELLS IN A PATIENT WITH TUBEROUS SCLEROSIS
Authors: Ishibashi Yasumasa , Inoue Yukiko , Takehara Kazuhiko , Furue Masutaka , Kukita Atsushi 
Published: April 2015

ABSTRACTSkin fragments of a giant molluscum pendulum and so‐called adenomata sebacea (AS) in the face of a 21‐year‐old female with Pringle's disease were explanted and the cell divisions of outgrown and subcultured non‐epithelial cells (NECs) were observed at suitable intervals with a phase contrast microscope. In both tissues, a number of NECs, especially large and medium sized ones, showed distinct chromosome dysarrangements, including segmental arrangement, eccentric arrangement, and arrangement failure, when chromosomes were arranged in the central area of individual cells in metaphase, causing extreme lagging in the subsequent cell division.These cells showed varying disturbances such as 1) the formation of elevated humps of varying sizes, followed by division after some of them were eliminated, 2) division into two cells of different sizes, 3) division into three after microcells had formed, 4) division into several, which was followed by restitution into two by fusion, 5) division failure, and 6) multinucleation after division, all of which suggested C‐mitosis.These findings indicate that the distributing mechanism of chromosomes, i.e. the centromere‐microtubules‐centriole system, particularly the function of microtubules, might be disturbed in the NECs.
LABORATORY STUDIES OF COLLAGEN WOUND DRESSING (CAS)
Authors: Murata Shinjiro , Fujigaki Masao , Umemura Koshiro , Izawa Masanori , Shibata Uichi , Fujii Tadahiko , Nishizawa Masaru , Iwamoto Kuniharu , Matsumoto Katsumi 
Published: April 2015

ABSTRACTIn order to elucidate the behavior of collagen wound dressing (CAS) after application, the transport of radioactivity from 3H‐CAS applied to rabbit skin excision was determined.Neutral soluble collagen fraction was extracted from the skin of 30 rats given 30 mCi of 3H‐glycine, followed by crosslinking with glutaraldehyde. The resulting mixture was then used as a binder of unlabelled collagen web to form 3H‐CAS.Skin, 2.5 cm square and 0.02 inch deep, was excised with a dermatome on bilateral sites of the back of 6 rabbits.Then, 3H‐CAS sheet, 3.5 cm square, was applied to each excision with 20 pieces of gauze and an elastic bandage. Each animal was kept separately in a metabolic cage to collect urine and feces every 24 hours. After the animals were sacrificed on the 8th day, 3H‐CAS, gauze, elastic bandage, and the skin near the treatment area were collected, and the radioactivity was determined by a liquid scintillation counter after combustion in a sample oxidizer.

More than 98.2% of radioactivity was recovered from 3H‐CAS after 8 days.
Release of radioactivity from 3H‐CAS by exudate was negligible and percutaneous transport of collagen was not observed.
No radioactivity was detected in rabbit serum, urine, or feces.
INITIAL STAGE OF PROCESSING FROM PREKERATIN INTO KERATIN MOLECULES IN HUMAN EPIDERMIS IN VITRO
Authors: Katagata Yohtaro , Aso Kazuo 
Published: April 2015

ABSTRACTIn order to clarify the initial formation of keratin from prekeratin molecules, we undertook a pulse‐chase experiment using [U‐14C]‐glycine labelled prekeratin subunit mixture in vitro.The experiment was carried out at 37°C for 3 hours using a specimen (3 mm2×0.1 mm) in Dulbecco minimum essential medium (MEM) containing 14C‐glycine. Incorporation of 14C‐glycine into the prekeratin molecules was exhibited in six prekeratins (M.W. about 71, 69, 62, 55, 52 and 49 K, respectively), and the highest incorporated molecule among them was the 55K‐prekeratin molecule. After 30 minutes at 37°C in the above medium, the intact specimens were transferred into a comparable medium free of 14C‐glycine, and further incubated for 4 hours. The 52K‐ and 62K‐prekeratin molecules had increased at 60 minutes after being chased, as compared with the starting radioactive value. From this result, it was suggested that the two prekeratin molecules might be intermediates in the initial stage of keratinization. Moreover, the human epidermal homogenate was more effective in converting the prekeratin into keratin molecules. Some factor(s) may be present in the fraction which convert the prekeratin into keratin molecules.
ECCRINE POROCARCINOMA
Authors: Claudy A.L. , Garcier F. , Kanitakis J. 
Published: April 2015

ABSTRACTThe histological, electron microscopic, and immunological findings in a case of eccrine porocarcinoma are described. The tumors were verrucous, wart‐like lesions and nodules which ulcerated secondarily. They were located on the leg. The patient died in a few months from dissemination of the disease. The tumor examined was epidermotropic and mainly composed of a clear cell proliferation. Ultrastructurally, the cells showed large, nucleolated nuclei, numerous mitochondria, dilated endoplasmic reticulum, single desmosomal plaques, sparse tonofibrils in the perinuclear region, multivesicular dense bodies, and interdigitating cytomembranes. A few dark cells were present. No Langerhans cells, indeterminate dendritic cells or melanocytes were observed. The tumor cells were labeled with KL 1 monoclonal antikeratin antibody. The secretory cells of uninvolved eccrine sweat glands in the close vicinity of the tumor and in the perilesional skin were strongly labeled with D 47 monoclonal antibody reacting with the secretory cells of human eccrine sweat gland, whereas the tumor cells remained unlabeled. Our study results showed that the origin of this tumor was not the secretory portion of the gland but rather its excretory portion.
ECTOPIC NAIL ASSOCIATED WITH BONE DEFORMITY
Authors: Yamasaki Reiko , Yamasaki Masahiro , Kokoroishi Takatoshi , Jidoi Joji 
Published: April 2015

ABSTRACTA 5‐year‐old girl with a congenital ectopic nail is reported. The nail existed on the tip of left little finger. The distal phalanx showed an M‐shaped deformity on the roentgenogram. After an operation for resectioning of the ectopic nail, the depression between the two peaks of the distal phalanx became shallow. The necessity of a roentgenologic observation of the ectopic nail, especially in this child's case, was considered.
CLEAR CELL ACANTHOMA DEVELOPING IN EPIDERMAL NEVUS
Authors: Sawada Mitsuo , Ishizaki Hiroshi 
Published: April 2015

ABSTRACTA clear cell acanthoma developed in an epidermal nevus of a 73‐year‐old man who had the Leser‐Trélat sign in association with lung cancer. The occurrence of clear cell acanthoma is presumably related to the pathogenesis of the Leser‐Trélat sign.
STUDIES ON BLACKFOOT DISEASE AND CHRONIC ARSENISM IN SOUTHERN TAIWAN
Authors: Yu Hsin‐Su , Sheu Hamn‐Ming , Ko Spring‐Shiang , Chiang Lien‐Chai , Chien Chung‐Ho , Lin Shan‐Meei , Tserng Biing‐Rong , Chen Chin‐Shu 
Published: April 2015

ABSTRACTAn endemic peripheral vascular disorder called “blackfoot disease” has been noticed in a limited area on the southern coast of Taiwan. Clinically, the symptoms and signs of blackfoot disease (BFD) are similar to those of Buerger's disease. The main ultrastructural change in the dermal blood vessel is degeneration of the endothelial cells. Among the inhabitants of this endemic area, a remarkable percentage of chronic arsenism is also found. It is currently believed that drinking of artesian well water containing both a fluorescent substance (FC) and arsenic is the cause of these two diseases. The purpose of this study is to examine the vascular and mutagenic effects of FC and also to discuss the roles of FC and arsenic in BFD and chronic arsenism. It was found that FC has the following effects: (1) inhibition of histamine‐induced capillary permeability (2) degeneration in the capillary endothelial cells in vivo (3) mutagenesis, and (4) enhancement of the incorporation of 3H‐thymidine in low fetal calf serum containing cultured fibroblasts. This is strong evidence that FC is the primary substance affecting the endothelial cells and has an important effect in BFD. Other effects of FC include mutagenicity and increased DNA synthesis. When these effects are combined with arsenic, chronic arsenism becomes prevalent.
PORPHYRIA CUTANEA TARDA ASSOCIATED WITH LYMPHOMA
Authors: Adrados G. , Orejas B. , Salamanca R. Enriquez De , Aniceto J. , Valls V. , Aguiar A. Ruiz De , Fernandez‐Cruz A. 
Published: April 2015

ABSTRACTIn a patient with histiocytic diffuse lymphoma, the diagnosis of subclinical porphyria cutanea tarda was simultaneously established. Chemotherapy improved both conditions until a septic complication occurred.Although the association of the two diseases may have been casual, their possible relationship should be taken into account.
STRUCTURAL ABNORMALITIES IN THE MITIS FORM (TYPE‐II) OF EHLERS‐DANLOS SYNDROME
Authors: Masutani Mamoru , Unno Toshio 
Published: April 2015

ABSTRACTThe ultrastructure of collagen fibers in the dermis of Ehlers‐Danlos syndrome (EDS) has been described in several previous reports. However, discussions of the ultrastructural changes in EDS‐Type II have been infrequent and brief. The behavior of alterative degeneration remains unclear, although there has been one previous report each on EDS‐Type I, Type VI, and Type VII (1, 3, 6). Our ultrastructural studies of EDS‐Type II revealed that bundles of collagen fibers were slightly thinner than in normal human skin, as were the individual collagen fibers also. Two or three serrated collagen fibers (up to 188 nm) were seen within the collagen bundle. The width of these serrated fibers was clearly less than in EDS‐Type I, and these fibers were less frequent than in the severe clinical form of EDS. It could be useful to be able to classify the subtypes of the EDS by structural pattern as well as by clinical appearance.
AGE‐RELATED CHANGES IN DRUG CONCENTRATION IN SKIN AND LUNGS OF MICE
Authors: Ohkoshi Motohiro , Oka Tohru 
Published: April 2015

ABSTRACTHow drug concentration in skin and lung due to vascular action changed with aging was examined quantitatively by a bioassay using bacteria. The concentration of the drug (anticancerous agent, Bleomycin) did not decrease in the skin of older mice as compared with that in younger mice, but showed a decrease in the lungs.An experiment using Kallikrein, a peripheral vasodilator, revealed that the kinin‐kallikrein system exists in mice.The peripheral circulation increasing action of Kallikrein decreased in the skin and increased in the lungs of older mice as compared with young mice.
CLINICAL AND EXPERIMENTAL STUDIES ON AGGLUTININS TO SPOROTHRIX SCHENCKII IN NONSPOROTRICHOTIC HUMAN AND ANIMAL SERA
Authors: Kobayashi Hiroto , Ishizaki Hiroshi 
Published: April 2015

ABSTRACTSera from 348 nonsporotrichotic patients were tested for agglutinin titers to Sporothrix schenckii. Agglutinins in low titers (less than 1:64) were present in 96% of sera. Agglutinins were partially removed by absorption with group B and group G streptococci, but not with group C streptococci. Agglutinins were also partially inhibited by the monosaccharides, galactose, rhamnose or mannose. The decrease in agglutinin titers did not show any correlation with group B or G streptococcus absorptions, or with inhibitions by the three monosaccharides. A significant elevation of agglutinin titers in rabbit sera was seen after immunization with group B and G streptococci or Klebsiella pneumoniae K47 cells. From these data, it was suggested that agglutinins to S. schenckii in nonsporotrichotic humans and animals occur not from immunological response to S. schenckii but from a heterologous background.
ISOLATION OF A LOW MOLECULAR WEIGHT GLYCOPROTEIN IN THE HUMAN URINE: COMPARISON WITH URINARY PEMPHIGOID ANTIGEN
Authors: Miyagawa Sachiko , Yonemasu Kunio , Okuchi Toshie , Sakamoto Kuniki 
Published: April 2015

ABSTRACTA glycoprotein was isolated from the urine of normal individuals and patients with bullous pemphigoid by means of ultrafiltration, ion exchange chromatography, and gel filtration. The yield of the purified glycoprotein from each liter of pooled urine was 0.35–0.40 mg. Analysis of the glycoprotein on SDS‐polyacrylamide gel electrophoresis showed a single band of approximate molecular weight 23 kd. The glycoprotein contained approximately 2.5% (w/w) carbohydrate. The glycoprotein differed from the urinary pemphigoid antigen described by Diaz et al (1), though both were nondialyzable, cathodally migrating, low molecular weight glycoproteins in the human urine. The significance of the glycoprotein is unclear at present, but it might be useful for studies of the metabolic degradation process of serum proteins.
PUNCH BIOPSY OF THE SKIN: EFFECT OF TISSUE FIXATION WITH CO2‐FREEZING
Authors: Serup Jørgen 
Published: April 2015

ABSTRACTThe effect of tissue fixation with CO2‐freezing on the size and quality of punch biopsies was studied post mortem. Four millimeter punch biopsies were taken from the abdominal skin with a motor drill operating at 250 RPM, before and after freezing with CO2 in acetone. The dry defatted weight was determined from 32 biopsies in each series, and light microscopy was performed on 16 biopsies in each series after setting up a double blind system.The dry weight of the whole biopsy was higher (p<0.05) after CO2‐freezing, but the weight of the epidermis/dermis alone was the same. The conical shape of the punch biopsies was due to retraction occurring during and after biopsy and not to loss of tissue.In the CO2‐frozen series, there were more (p<0.001) biopsies cylindrical in shape, more (p<0.001) biopsies including fascia and muscle, fewer (p<0.001) biopsies with trauma, more (p<0.01) biopsies with subcutaneous tissue represented, in a larger amount (p<0.001), and more (p<0.001) biopsies optimal for microscopy.After tissue fixation, the quality of the biopsies for microscopy was much better with regard to tissues represented and tissue archilecture. Further efforts to develop an easy in vivo technique for tissue fixation are indicated.
SKIN PENETRATION OF NICOTINIC ACID, METHYL NICOTINATE, AND BUTYL NICOTINATE IN THE GUINEA PIG
Authors: Osamura Hiromi , Jimbo Yuko , Ishihara Masaru 
Published: April 2015

ABSTRACTCarbon‐14 labeled nicotinic acid (NA), methyl nicotinate (MN), and butyl nicotinate (BN) were dissolved in distilled water or olive oil vehicles and applied to in vivo and excised guinea pig skin. Four skin conditions were represented by allergic, irritated, tape‐stripped, and normal skin. The amount of chemicals released to, retained by, and penetrating the skin was measured one‐half to twenty‐four hours after application.The penetration of NA was less than that of BN or MN. The release of chemicals to the skin was inversely related to their solubility in vehicles. More chemical was released to in vivo than excised skin. A barrier effect occurring in the lowest part of the horny layer affected NA most, followed by BN, and MN. Release of NA and MN from vehicles was greatest in tape‐stripped skin, followed by irritated, allergic, and normal skin. BN release was not affected by skin conditions.
SARCOIDOSIS WITH XANTHOMATHOUS AND NECROBIOTIC CHANGES
Authors: Fujii Yoshihisa , Shinkai Hiroshi , Takayasu Susumu 
Published: April 2015

ABSTRACTA 74‐year‐old female patient with sarcoidosis who had cutaneous lesions on the scalp and lower legs, cervical lymph node enlargement, and bilateral hilar lymphadenopathy was studied. Histologically, aggregates of foam cells intermingled with lymphoid cells in addition to epithelioid cell granulomas were detected in the atrophic plaque with yellowish macules of the scalp. Additionally, she had brownish nodules on the lower legs with degenerative changes of connective tissue surrounded by epithelioid cell granulomas. In this degenerative area, irregularly clumped elastic fibers were observed. These elastic fibers were stained positively by the von Kossa method.
EFFECTS OF MOUSE AND HUMAN EPIDERMAL GROWTH FACTOR ON THE OUTGROWING EPIDERMIS OF PIG AND HUMAN SKIN EXPLANTS
Authors: Aoyagi Takashi , Kato Naoko , Suya Hideko , Miura Yusho , Yoshida Tetsunori , Ohura Takehiko 
Published: April 2015

ABSTRACTThe effects of mouse and human epidermal growth factors (mEGF and hEGF) on the outgrowing epidermis of pig and human skin explants were studied. Both mEGF and hEGF significantly stimulated the rate of pig and human epidermal outgrowth at 0.01–0.05 µg/ml. mEGF also stimulated mitosis of pig outgrowing epidermis. The stimulatory effect of mEGF on the rate of pig epidermal outgrowth and mitosis of pig outgrowing epidermis was potentiated by fetal calf serum. These results indicate that EGF may have a possibile clinical application in epidermal wound healing.
EFFECT OF PLANT SH‐PROTEASE ON HUMAN POLYMORPHONUCLEAR LEUKOCYTE GRANULE ELASTASE
Authors: Hamanaka Kazuko , Toki Naotika , Takasugi Sumiyoshi , Sumi Hiroyuki 
Published: April 2015

ABSTRACTIn the present study, attempts were made to examine the effects of plant SH‐protease on human polymorphonuclear leukocyte granule elastase. S‐2484 amidolytic activity in extracted solution using 0.1% Triton X‐100 was remarkably higher than in the solution without Triton X‐100. The authors confirmed the possibility that the presence of solvents such as Triton X‐100 might be required for extraction of elastase from the granule membrane. It was also confirmed that ficin and papain inhibited extraction of elastase from the granule membrane by Triton X‐100, and that SH‐protease can inhibit S‐2484 amidolytic and Congo red‐elastinolytic activities of the released elastase. However, it is not clear whether SH‐protease inhibits the release of proelastase from the granule membrane or inhibits activation of released proelastase to elastase. The authors assume that there may be competitive inhibition between SH‐protease and elastase against the substrate.
EXPERIMENTAL CUTANEOUS VASCULITIS IN HAMSTER CHEEK POUCH CHAMBERS
Authors: Sarashi Chizuko , Nishioka Kiyoshi , Katayama Ichiro , Sano Shigeharu 
Published: April 2015

ABSTRACTInduction of cutaneous vasculitis has been attempted in hamster cheek pouch chambers. Only slugging of blood flow and narrowing of blood vessels were noted in the chambers by injecting a mixture of antigen and antibody at an antibody excess ratio. Hemorrhagic reaction was observed at venules connecting newly growing capillaries to epidermal grafts. Massive infiltration of polymorphonuclear leukocytes was noted at hemorrhagic sites where deposition of the antibody on the blood vessel wall was found. As hemorrhagic response was observed in half of the hamsters injected with the mixture and in those preimmunized and then injected with antigens, our system will provide an aid in analyzing human cutaneous vasculitis.