bluuebunny/crossref_metadata_2025_split huggingface dataset:
Indirect in vitro Regeneration in Four Varieties of Potato (Solanum tuberosum L.) from Internodal Segments and Leaf Explants
Authors: Mollika Sanjida Rahman , Islam Tahmina , Hoque M I , Sarker R H 
Published: July 2024

Internodal segments and leaf explants from four varieties of potato (Solanum tuberosum L.) cultivated in Bangladesh, namely, Asterix, Diamant, Granola and Lady Rosseta were utilized for indirect in vitro regeneration of shoots through callus culture. The best responses regarding the reaeration of shoots were obtained when the explants were cultured on MS medium supplemented with 4.0 mg/l BAP and 1.0 mg/l IAA and 0.5 mg/l GA3. The highest frequency of multiple shoot regeneration was recorded from internodal segments rather than from the leaf explants. Hormone free MS medium was found to be the most effective for healthy root induction from the in vitro raised excised shoots. Following adequate root induction, the in vitro regenerated plantlets were successfully transplanted and established to soil for further growth and development of tubers.
Plant Tissue Cult. & Biotech. 34(1): 83-92, 2024 (June)
Investigation of the rock mass state in the near-wall part of the quarry and its stability management
Authors: Saik Pavlo , Rysbekov Kanay , Kassymkanova Khaini-Kamal , Lozynskyi Vasyl , Kyrgizbayeva Guldana , Moldabayev Serik , Babets Dmytro , Salkynov Arnat 
Published: July 2024

Introduction: The research is aimed to identify the vulnerable areas of quarry slopes through the creation of a stress-strain state model for the rock mass at the Vostochny quarry, East Saryoba field of the Zhi-landy Group of cupriferous sandstones, as well as their subsequent strengthening.Methods: The research is based on the development of a database containing information on mining-geological, geophysical, geodetic and aerospace surveys. The authors of the research use modern geodetic, aerospace and geophysical technologies for scientifically based predicting of technogenic disasters and the rock mass stress-strain state modeling to ensure safe and optimal mining of fields in difficult mining-geological conditions.Results: The results obtained show that the strengthening of weakened quarry slopes based on the rock mass stress-strain state modeling contributes to the scientific-practical profitability of field mining and provides safe mining in difficult mining-geological conditions.Discussion: Novelty is in the scientific substantiation of the method for strengthening quarry slopes, which is based on the rock mass stress-strain state modeling. The research is of great practical importance, as the rock mass stress-strain state modeling increases the reliability of predicting the rock mass state during its mining. Strengthening of quarry slopes using the proposed method reduces risks and increases safety and economic efficiency of mining the solid mineral deposits in difficult mining-geological conditions.
The growzen™ buddy smartphone app to improve adherence in patients receiving recombinant human growth hormone therapy: a retrospective observational study in Argentina
Authors: van Dommelen Paula , Arnaud Lilian , Assefi Aria Reza , Crespo Maria Lourdes , Debicki Matias , Michelis Fulvio , Koledova Ekaterina 
Published: July 2024

IntroductionThis study in Argentina evaluated the impact of the growzen™ buddy smartphone app on adherence to recombinant human growth hormone (r-hGH) treatment.MethodsThe adherence data, invitation dates with a link to the app, app activation dates, and height measurements entered were extracted from the growzen™ digital health ecosystem. Patients with 12 months of adherence data, aged ≥2 years at treatment start, and aged &lt;19 years were selected both before and after app implementation. Mean adherence was classified as optimal (≥85%) versus suboptimal (&lt;85%). Adherence before and after implementation and the pre–post effect on adherence were assessed.ResultsData for 830 patients were available. Prior to app implementation, the proportion of patients with optimal adherence was 68% (n = 348/515). Following the app implementation, out of 315 patients, 302 (96%) received an invitation with a link to the app, 225 (71%) activated their account, and 127 (40%) entered height data in the first year. There was a significant early increase in the proportion of patients with optimal adherence following implementation: 82% (n = 258/315), p &lt; 0.001. After implementation, the proportion of patients with optimal adherence included 80% (n = 78/98) of those with an active account who did not enter height measurements and 89% (n = 113/127) of those who did. There was a significant and positive pre–post app effect on adherence (p &lt; 0.01) in patients with an active account.DiscussionOur results show that using the growzen™ buddy app has a rapid and positive impact on adherence to r-hGH treatment, and patients who were more engaged with the app demonstrated better adherence.
Treatment of lower cervical spine fracture with ankylosing spondylitis by simple long anterior cervical plate: a retrospective study of 17 cases
Authors: Chen Weifu , Yang Yu , Pan Wenjun , Lei Xinhuan , Hong Zhenghua , Luo Hua 
Published: July 2024

ObjectiveAnkylosing spondylitis (AS), an autoimmune disease, often leads to lower cervical spine fractures, with the potential for severe spinal nerve damage even from low-energy injuries. The optimal treatment approach remains debated.MethodsA retrospective study involved 17 AS patients with lower cervical spine fractures who received anterior cervical fixation. Most presented cervicothoracic or thoracolumbar kyphosis, with 11 exhibiting neurological deficits. Patient characteristics, clinical data, visual analog scale (VAS), complications, and nerve recovery were analyzed.ResultsNo postoperative neurological deterioration occurred. All cases experienced complete fusion of fractures during the follow-up period. Preoperative VAS significantly decreased at 3 days and 3 months post-surgery. Of the 11 patients with preoperative neurological deficits, approximately 54.5% showed improvement post-surgery. No complications were reported, such as esophageal fistula, wound infection, or fixation failure.ConclusionAnterior internal fixation is a possible treatment for AS-related lower cervical fractures. This approach ensures satisfactory spinal stability and neurological recovery with proper cranial traction and external fixation post-surgery. Our findings demonstrate that this surgical method is safe and effective.
Video-based diagnosis support system for pianists with Musician’s dystonia
Authors: Oku Takanori , Furuya Shinichi , Lee André , Altenmüller Eckart 
Published: July 2024

BackgroundMusician’s dystonia is a task-specific movement disorder that deteriorates fine motor control of skilled movements in musical performance. Although this disorder threatens professional careers, its diagnosis is challenging for clinicians who have no specialized knowledge of musical performance.ObjectivesTo support diagnostic evaluation, the present study proposes a novel approach using a machine learning-based algorithm to identify the symptomatic movements of Musician’s dystonia.MethodsWe propose an algorithm that identifies the dystonic movements using the anomaly detection method with an autoencoder trained with the hand kinematics of healthy pianists. A unique feature of the algorithm is that it requires only the video image of the hand, which can be derived by a commercially available camera. We also measured the hand biomechanical functions to assess the contribution of peripheral factors and improve the identification of dystonic symptoms.ResultsThe proposed algorithm successfully identified Musician’s dystonia with an accuracy and specificity of 90% based only on video footages of the hands. In addition, we identified the degradation of biomechanical functions involved in controlling multiple fingers, which is not specific to musical performance. By contrast, there were no dystonia-specific malfunctions of hand biomechanics, including the strength and agility of individual digits.ConclusionThese findings demonstrate the effectiveness of the present technique in aiding in the accurate diagnosis of Musician’s dystonia.
Longitudinal changes in Mediterranean diet adherence and perceived benefits and barriers to its consumption in US university students
Authors: Yildiz Serhat , Downing Patrick , Knight Caroline J. , Frugé Andrew D. , Greene Michael W. 
Published: July 2024

ObjectiveThe Dietary Guidelines for Americans has recommended consumption of a Mediterranean diet (MD) for overall health and wellbeing, and the US News &amp; World Report has ranked the MD as the top diet overall for the past six consecutive years. However, it is uncertain if university students in the United States (US) have increased their adoption of this dietary approach over these past six years.DesignLongitudinal cross-sectional survey conducted in three cohorts (2018, 2020, 2022) utilizing regression models to assess MD Adherence and other relevant outcomes variables.SettingUniversity in the southern US.ParticipantsStudents (n = 761) enrolled in undergraduate introductory nutrition course.ResultsSurvey respondents were 83% female, 91% white, and 97% ages 18–24. Predictors of MD adherence were older age, female gender, and health-related qualifications. MD adherence was lowest in 2022. The 2022 group perceived less MD health benefits, weight loss, ethical concerns, natural content, and sensory appeal compared to the 2018 group. During the COVID-19 pandemic, changes in eating behavior were examined in the 2020 and 2022 groups. We observed that participants in the 2022 group had a greater frequency of snacking and a lower frequency of eating out compared to 2020 group.ConclusionMD adherence did not increase over time in US university students. These findings underscore the need for targeted interventions and education to promote healthier eating habits in university students.
Does a high peritoneal cancer index lead to a worse prognosis of patients with advanced ovarian cancer?: a systematic review and meta-analysis based on the latest evidence
Authors: Wang Siyu , Liu Shaoxuan , Liu Fangyuan , Guo Ying , Han Fengjuan 
Published: July 2024

BackgroundThe newest clinical evidence that the relationship between the peritoneal cancer index (PCI) and the postoperative prognosis of advanced ovarian cancer patients remains controversial, and there are no large-sample and multicenter studies to clarify this matter. Therefore, in this paper, we used meta-analysis to systematically assess the postoperative prognostic value of PCI in subjects with advanced ovarian cancer to provide individualized treatment plans and thus improve the prognosis of patients.MethodsLiterature on the correlation between PCI and the postoperative prognosis in subjects with advanced OC undergoing cytoreductive surgery (CRS) was searched in the Cochrane Library, Pubmed, Embase, and Web of Science from the database inception to April 20, 2023. The search was updated on February 28, 2024. We only included late-stage (FIGO stage: III-IV) patients who did not undergo neoadjuvant chemotherapy (NACT) or hyperthermic intraperitoneal chemotherapy (HIPEC). Afterwards, literature screening and data extraction were conducted using Endnote20 software. The literature quality was assessed using the Newcastle-Ottawa Scale (NOS). Lastly, statistical analysis was performed with STATA 15.0 software.ResultsFive studies with 774 patients were included. The result indicated that patients with high PCI had a worse prognosis than those with low PCI. The combined hazard ratio was 2.79 [95%CI: (2.04, 3.82), p&lt;0.001] for overall survival (OS) in patients with high PCI. Further subgroup analysis by the FIGO staging revealed that in stage III [HR: 2.61, 95%CI: (2.00, 3.40), p&lt;0.001] and stage III-IV patients [HR: 2.69, 95%CI: (1.66, 4.36), p&lt;0.001], a high PCI score was significantly associated with a worse prognosis. The PCI score had a greater impact on the OS of patients with higher stages. The combined hazard ratio was 1.89 [95%CI: (1.51, 2.36), p&lt;0.001] for progression-free survival (PFS) in patients with high PCI.ConclusionPCI may be used as a postoperative prognosis indicator in patients with advanced OC on primary debulking surgery. High PCI indicates a worse prognosis. However, further research is warranted to confirm these findings.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023424010.
Obstructive or non-obstructive megacystis: a prenatal dilemma
Authors: Mandaletti Martina , Cerchia Elisa , Ruggiero Elena , Teruzzi Elisabetta , Bastonero Simona , Pertusio Annasilvia , Della Corte Marcello , Sciarrone Andrea , Gerocarni Nappo Simona 
Published: July 2024

IntroductionDiagnosis of prenatal megacystis has a significant impact on the pregnancy, as it can have severe adverse effects on fetal and neonatal survival and renal and pulmonary function. The study aims to investigate the natural history of fetal megacystis, to try to differentiate in utero congenital lower urinary tract obstruction (LUTO) from non-obstructive megacystis, and, possibly, to predict postnatal outcome.Materials and methodsA retrospective single-center observational study was conducted from July 2015 to November 2023. The inclusion criteria were a longitudinal bladder diameter (LBD) &gt;7 mm in the first trimester or an overdistended/thickened-walled bladder failing to empty in the second and third trimesters. Close ultrasound follow-up, multidisciplinary prenatal counseling, and invasive and non-invasive genetic tests were offered. Informed consent for fetal autopsy was obtained in cases of termination of pregnancy or intrauterine fetal demise (IUFD). Following birth, neonates were followed up at the same center. Patients were stratified based on diagnosis: LUTO (G1), urogenital anomalies other than LUTO (“non-LUTO”) (G2), and normal urinary tract (G3).ResultsThis study included 27 fetuses, of whom 26 were males. Megacystis was diagnosed during the second and third trimesters in 92% of the fetuses. Of the 27 fetuses, 3 (11.1%) underwent an abortion, and 1 had IUFD. Twenty-three newborns were live births (85%) at a mean gestational age (GA) of 34 ± 2 weeks. Two patients (neonates) died postnatally due to severe associated malformations. Several prenatal parameters were evaluated to differentiate patients with LUTO from those with non-LUTO, including the severity of upper tract dilatation, keyhole sign, oligohydramnios, LBD, and GA at diagnosis. However, none proved predictive of the postnatal diagnosis. Similarly, none of the prenatal parameters evaluated were predictive of postnatal renal function.DiscussionThe diagnosis of megacystis in the second and third trimesters was associated with live births in up to 85% of cases, with LUTO identified as the main cause of fetal megacystis. This potentially more favorable outcome, compared to the majority reported in literature, should be taken into account in prenatal counseling. Megacystis is an often misinterpreted antennal sign that may hide a wide range of diagnoses with different prognoses, beyond an increased risk of adverse renal and respiratory outcomes.
Evaluation of phrenic nerve stimulation trigger lag and synchronization in different modes of ventilation
Authors: Bentley Ishmael , Jocewicz Frank T. , Johnson Bruce D. , Mehta Hitesh P. 
Published: July 2024

Phrenic nerve stimulation is currently being investigated for the prevention of diaphragm atrophy in patients with mechanically supported breathing. Patients receiving breathing support from mechanical ventilation are at risk of mismatches between respiratory demand and ventilator support. Our objectives were to determine if a novel phrenic nerve stimulation device provided stimulation during inspiration as intended and did not exacerbate any potential discordances. A benchtop electromechanical simulation model was developed to validate phrenic nerve stimulation with simulated breathing. The phrenic nerve stimulation device was evaluated with a mechanical ventilator attached to a breathing simulator. The trigger ratio and time lag between phrenic nerve stimulation and mechanical ventilation was measured for multiple disease and ventilator parameters. For the 1:1 breath trigger ratio test, 99.79% of intended stimulation breaths received stimulation at the correct time. For the 1:4 breath trigger ratio test, 99.72% of intended stimulation breaths received stimulation at the correct time. For trigger lag times for the inspiratory and expiratory phases, the mean inspiratory lag was 36.10 ± 10.50 ms and 16.61 ± 3.61 ms, respectively. The following discordance scenarios were evaluated in conjunction with simulated phrenic nerve stimulation: asynchrony-false trigger, dyssynchrony-early trigger, dyssynchrony-late trigger, dyssynchrony-early cycling, dyssynchrony-late cycling. Testing demonstrated none of these discordances were exacerbated by the simulated phrenic nerve stimulation. The novel phrenic nerve stimulation device delivered electrical stimulation therapy as intended and did not exacerbate any simulated discordances.
Association between polyunsaturated fatty acids and depression in women with infertility: a cross-sectional study based on the National Health and Nutrition Examination Survey
Authors: Hong Yan , Jin Xia , Shi Lihong 
Published: July 2024

BackgroundIdentifying possible influencing factors is crucial for the depression symptoms of women experiencing infertility. This study aims to explore the association between polyunsaturated fatty acids (PUFAs) and the odds of depression symptoms in women experiencing infertility.MethodsThis is a cross-sectional study based on the National Health and Nutrition Examination Survey (NHANES). PUFA intake was obtained through a 24-h dietary recall interview. Depression symptoms were defined using the Patient Health Questionnaire-9 (PHQ-9) with a score of ≥10 points or as taking antidepressants. The association between PUFA and depression was assessed using a logistic regression model by calculating the odds ratio (OR) with 95% confidence interval (CI). Subgroup analysis was carried out based on menopausal status and female hormone use.ResultsThere were 725 participants included for analysis. After adjusting the covariables, lower odds of depression symptoms were found in patients with the intake of omega-3 PUFA (OR = 0.48, 95% CI: 0.24–0.96) and omega-6 PUFA (OR = 0.24, 95% CI: 0.14–0.42) in the second tertile (T2) in comparison to the first tertile (T1). The intake of α-linolenic (ALA) (OR = 0.48, 95% CI: 0.23–0.97) and linoleic acid (OR = 0.24, 95% CI: 0.14–0.41) in T2 was also found to be related to the reduced odds of depression symptoms in comparison to T1.ConclusionsOur findings suggest a potential association between moderate omega-3 and omega-6 PUFA intake and a reduced risk of depression symptoms in women experiencing infertility. This implies that clinicians might find it useful to consider dietary advice that includes PUFA-rich foods as part of a broader strategy to address mental health in this patient group. However, further research is needed to confirm these preliminary findings and to establish the optimal levels of PUFA intake for mental health benefits.
Long-term evacuation and obesity: a 12-year follow-up comparative study of residents inside and outside Katsurao Village after the Fukushima nuclear disaster
Authors: Ito Naomi , Takita Morihito , Moriyama Nobuaki , Amir Isamu , Furuyama Ayako , Saito Hiroaki , Abe Toshiki , Yamamoto Chika , Sato Mika , Zhao Tianchen , Tsubokura Masaharu 
Published: July 2024

ObjectiveEvacuation, owing to a disaster, impacts various aspects of an individual's life, including health status. This study aimed to determine the prevalence of obesity among residents of Katsurao Village, Fukushima Prefecture, after the evacuation order due to the Fukushima nuclear disaster in 2011 was lifted in 2016 and to compare the prevalence of obesity by place of residence (inside or outside the village).MethodsThe number of examinees, sex, age, place of residence, body mass index (BMI), exercise habits, smoking habits, drinking habits, and dietary status were extracted from the results of health checkups since 2016. We compared the BMI of the indigenes of Katsurao Village by place of residence (inside or outside the village) over time.ResultsAlthough 7 years have passed since 2016, ~70% of the registered residents of Katsurao Village still live outside the village. The obesity rates have consistently been higher among people living outside the village compared to those inside, and the place of residence was the only factor significantly associated with obesity.ConclusionThe findings of this study suggest early intervention is necessary to prevent health risks associated with disaster evacuation if the evacuation period is prolonged.
Knowledge, practice, and infection status of prisoners toward dermatomycosis: a study in Nepal
Authors: Dhakal Bikrant , Shrestha Bhagat Lal , Shah Sangam , Rijal Shrija , Karki Bibek Bahadur , Pangeni Paras Modi , Bhatta Nikita , Mehta Rachana , Thapa Neeraj , Zeeshan Bushra , Sah Ranjit , Luna Camila , Bonilla-Aldana D. Katterine , Rodriguez-Morales Alfonso J. 
Published: July 2024

BackgroundDermatophytosis, commonly known as tinea, poses a significant public health concern worldwide, especially in environments with poor hygiene and overcrowding, such as prisons. Despite its prevalence and impact on quality of life, there is a lack of research on the knowledge and hygiene practices regarding dermatophytosis among prisoners, particularly in Nepal.ObjectiveThe study aimed to assess prisoners’ knowledge, hygiene practice and infection status regarding dermatophytosis in Central Prison, Nepal.MethodsA descriptive cross-sectional study with a sample size of 184 respondents was designed to collect data using a validated pre-tested questionnaire from September 2023 to January 2024. The collected data was then analyzed using IBM SPSS version 21. Knowledge and hygiene practices were measured on an eight and 11-point scale and rated as poor (≤4) and sound (&gt;4), bad (≤6), and good (&gt;6), respectively. Summary data were presented by descriptive, while Chi-square and logistic regression were used for inferential statistics at p &lt; 0.05.ResultsThe findings revealed moderate knowledge among prisoners regarding dermatophytosis, with significant gaps in understanding its spread and prevention. While most prisoners recognized the importance of treatment, there were misconceptions about the inevitability of contracting dermatophytosis and the role of personal hygiene.ConclusionDespite good knowledge levels, adherence to recommended hygiene practices was suboptimal, highlighting the need for targeted interventions. The study underscores the importance of addressing knowledge gaps, changing attitudes, and promoting hygienic practices to mitigate the burden of dermatophytosis among prisoners.