bluuebunny/crossref_metadata_2025_split huggingface dataset:
The genus Clathria from the Gulf of Mexico and Mexican Caribbean, with redescription and resurrection of Clathria carteri (Poecilosclerida: Microcionidae)
Authors: GÓMEZ PATRICIA 
Published: April 2014

The present study deals with the morphologic variability of eight Clathria species from the southern Gulf of Mexico and Mexican Caribbean. Clathria (Clathria) foliacea, C. (C.) carteri, C. (Microciona) calla, C. (M.) echinata, C. (M.) spinosa, C. (Thalysias.) venosa, and C. (T.) virgultosa were collected by scuba diving and dredging from the southern Gulf of Mexico (Veracruz, Campeche Bank, Yucatan) to the Caribbean coast (Quintana Roo) from shoreline to 120 m depth. The population of Clathria seems to be neither abundant nor diverse in the studied area. However, the seven species considered here are presumed to have a slight morphologic variability, and the interspecific relationships had not been fully resolved. The present study focuses on their differentiation through comparison of external morphology, skeletal architecture, spiculation and measurements of characters as well as scanning electronic microscopy. Comparisons were made among intra- and interspecific material and with the available type material. In particular, C. carteri Topsent (1889), previously synonymized with C. foliacea, is resurrected by establishing a neotype from material from the type locality, Campeche Bank; this has not been studied since its original description and is here redescribed for the first time. Another neotype is assigned for C. foliacea from Campeche also, since the original material is no longer available, and this species is redescribed also. Significant differences in spiculation between C. foliacea and C. carteri were found by one-way ANOVA. Although C. (T.) venosa resembles C. (T.) raraechelae in spiculation, these two species differ in measurements and skeletal organization. C. (T.) virgultosa is typified by a peculiar acanthostyle that is markedly spined on distal parts of the shaft, and by two types of tiny microscleres. This study has extended the known geographic distribution of each of these Clathria spp. within the lower Gulf of Mexico.
Changes in Tropical Pacific Thermocline Depth and Their Relationship to ENSO after 1999
Authors: Wen Caihong , Kumar Arun , Xue Yan , McPhaden M. J. 
Published: April 2014

Abstract
The characteristics of El Niño–Southern Oscillation (ENSO) variability have experienced notable changes since the late 1990s, including a breakdown of the zonal mean upper-ocean heat content as a precursor for ENSO. These changes also initiated a debate on the role of thermocline variations on the development of ENSO events since the beginning of the twenty-first century. In this study, the connection between thermocline variations and El Niño and La Niña events is examined separately for the 1980–98 and 1999–2012 periods. The analysis highlights the important role of thermocline variations in modulating ENSO evolutions in both periods. It is found that thermocline variation averaged in the central tropical Pacific, including both equatorial and off-equatorial regions, is a good precursor for ENSO evolutions before and after 1999, while the traditional basinwide mean of equatorial thermocline variation is a good precursor only before 1999. The new precursor, including both high-frequency variability in equatorial regions and low-frequency variability in off-equatorial regions, is found to be indicative of multiyear persistent warm and cold conditions in the tropical Pacific. Further, it is found that the strength of the subtropical cells (STCs) interior mass transport in both hemispheres increased rapidly around the late 1990s. It is proposed that the strengthened STC interior transports provide a pathway for the enhanced influence of off-equatorial thermocline variations on the development of ENSO events after 1999.
Lessons Learned from 20 Years of Diabetes Self-Management Research With Mexican Americans in Starr County, Texas
Authors: Brown Sharon A. , Hanis Craig L. 
Published: April 2014

Purpose The purpose is to provide an overview of a 20-year research program aimed at testing diabetes self-management education interventions culturally tailored for Mexican Americans residing in an impoverished rural community on the Texas-Mexico border. Methods The research program involved focus group interviews to obtain community input, pilot testing to refine instruments and interventions, and community-based randomized controlled trials to examine intervention effects. Here the authors summarize lessons learned related to the (1) overall effects of culturally tailored diabetes self-management education; (2) impact of culture on study design, intervention development, health outcomes, and community acceptance; (3) benefits of and findings from multiple focus groups held over time in the community; and (4) personal and cultural motivators for behavioral change that were evident among study participants. Results Postintervention reductions in A1C ranged from 1.4 to 1.7 percentage points. Individuals who attended ≥ 50% of intervention sessions achieved a 6–percentage point reduction in A1C. Intervention teams included bilingual Mexican American nurses, dietitians, and promotoras, all recruited from the local community. Focus group interviews indicated that a traditional promotora model was not acceptable to the participants who wanted knowledgeable health professionals, or perceived authority figures, to lead intervention sessions while promotoras provided logistical support. Free glucometers and strips, family participation, and interpersonal dynamics within intervention groups motivated individuals to make healthier lifestyle choices. Conclusions Culturally tailored diabetes interventions are effective in improving the health of socially disadvantaged minorities who bear a disproportional burden of type 2 diabetes, and these interventions are cost-effective.
Pitfalls of femoral Titanium Elastic Nailing
Authors: Salonen A. , Lahdes-Vasama T. , Mattila V. M. , Välipakka J. , Pajulo O. 
Published: April 2014

Background and Aims: Despite several potential complications of elastic intramedullary nailing, it is currently the treatment of choice for femoral diaphyseal fractures in school-aged children. This study aimed to critically evaluate the complications of titanium elastic nailing in pediatric femoral shaft fractures. Material and Methods: This study evaluated patients with a diaphyseal femoral fracture treated with titanium elastic nailing (TEN) in Tampere University Hospital in Finland. The study group included 32 children with a mean age of 9 years during a 5-year period, from 1 January 2003 to 31 December 2007. Data were collected from medical records and x-rays. Mean follow-up time was 42 months. Results: Of 32 patients, 9 (28%) reported a postoperative complication. Complications were associated with nail prominence in five (16%) patients and instability in four (12%) patients. In patients with nail prominence, the titanium elastic nailing–nail ends were unbent and 10–35 mm outside the cortex of the distal femur. The nail prominence caused pain and delayed knee mobilization until the nail was removed after a mean time of 4 months. In patients with fracture instability, the mean titanium elastic nailing–nail/medullary canal diameter ratio was 46% and periosteal callus formation was 5.4 mm at the first control. In those with stable fractures, the values were 66% and 9.2 mm, respectively. Conclusions: Based on this study, two types of pitfalls in a small volume center were found. Titanium elastic nail ends were left unbent and too long. We recommend palpating the nail ends to exclude nail prominence and to verify free movement of the knee after nail cutting and bending. Fracture instability was caused by inserting titanium elastic nailing–nails that were too narrow. To avoid this complication, careful preoperative planning to select the proper-size titanium elastic nailing–nails and intraoperative testing of fracture stability under continuous fluoroscopy after the operation is advised.
The renin–angiotensin–aldosterone system in adolescent offspring born prematurely to mothers with preeclampsia
Authors: Washburn Lisa K , Brosnihan K Bridget , Chappell Mark C , Diz Debra I , Gwathmey TanYa M , Nixon Patricia A , Russell Gregory B , Snively Beverly M , O’Shea T Michael 
Published: April 2014

Hypothesis/introduction: Preeclampsia is associated with alterations in the maternal renin–angiotensin–aldosterone system (RAAS), increased blood pressure (BP), and cardiovascular risk in the offspring. We hypothesized that preeclampsia is associated with alterations in the RAAS in the offspring that persist into adolescence. Materials and methods: We compared components of the circulating ( n = 111) and renal ( n = 160) RAAS in adolescents born prematurely with very low birth weight (VLBW) of preeclamptic (PreE) and normotensive (NoHTN) pregnancies. Multivariable linear regression was used to evaluate potential confounding and intermediate variables. Analyses were stratified by sex. Results: Adjusting for race and antenatal steroid exposure, male offspring of PreE mothers had higher circulating aldosterone than those of NoHTN mothers (adjusted mean difference = 109; 95% confidence limits: −9, 227 pmol/L). Further adjustment for current BMI attenuated this difference (adjusted mean difference: 93; 95% confidence limits: −30, 215 pmol/L). Conclusion: Among male preterm VLBW infants, maternal preeclampsia is associated with increased circulating aldosterone level in adolescence, which appears to be mediated in part by higher BMI.
Protective effect of truncated Na+/K+‐ATPase β on ischemia/reperfusion-induced renal injury in rats
Authors: Gong Huilin , Sun Jingjing , Xue Wujun , Tian Puxun , Ding Xiaoming , Yan Hang , Li Yang , Zheng Jin 
Published: April 2014

Renal ischemia/reperfusion(I/R) is an important injury part of ischemic acute renal failure, and it is also the main factor that affects the early functional recovery and the long-term survival of transplanted kidney in renal transplantation. In this study, we cloned and expressed truncated Na+/K+‐ATPase β(tNKAβ) and demonstrated that tNKAβ could activate NKA α subunit and induce protective effect on human kidney-2(HK-2) cells via PKCɛ signal pathway. The half maximum effective concentrations (EC50) of tNKAβ were 0.24 µM. Furthermore, the application of EAVSLKPT (PKCɛ inhibitor) could abolish the protective effect of tNKAβ in HK-2 cells subjected to ischemia/reperfusion. To identify the protective effect of tNKAβ against the I/R injury in the kidney, Sprague-Dawley rats were treated with tNKAβ (75 mg/kg) for 2 h before ischemia. The tNKAβ-treated group demonstrated a significant improvement in renal function with a lower serum creatinine and blood urea nitrogen (BUN) levels on postoperative days 1–6. Renal sections obtained from rats of the I/R group showed serious renal injury which included degeneration of tubular structure, tubular dilation, swelling and necrosis, luminal congestion, and muddy brown casts formed by sloughing of severely damaged tubular epithelial cells. However, sections of rats that were administered with tNKAβ 2 h before reperfusion showed marked reduction of the histological features of renal injury compared with kidneys that were subjected to I/R only. In conclusion, the protective effects of tNKAβ against renal I/R injury have been evaluated for the first time, and these protective effects may occur via stimulation of PKCɛ pathways.
Cyclosporine A protects podocytes via stabilization of cofilin-1 expression in the unphosphorylated state
Authors: Li Xiaoyan , Zhang Xiaoyan , Li Xuejuan , Wang Xuejing , Wang Suxia , Ding Jie 
Published: April 2014

Podocyte foot process (FP) is dysregulated in nephrotic syndrome. The effacement of podocyte FPs typically arises following perturbations in the actin cytoskeleton. Recent data suggest that the effects of calcineurin (CaN) inhibitor cyclosporine A (CsA) are independent of its effects on T-cells, and CsA has been identified as stabilizing the actin cytoskeleton through stabilizing synaptopodin in podocytes, and thereby directly reducing proteinuria. Other studies showed that CsA could regulate cofilin-1 directly within tubular epithelial cells. However, whether synaptopodin is the only target of CsA or whether the antiproteinuric role of CsA is played by regulating cofilin-1 in podocytes has not been studied. In the present study, changes in the expression and distribution of nephrin, synaptopodin, cofilin-1 and phosphorylated cofilin-1 (pho-cofilin-1) were detected in both puromycin aminonucleoside (PAN) induced nephrotic rats treated with CsA and cultured podocytes exposed to PAN with/without CsA. Cofilin-1, synaptopodin mRNA was knocked down or combined by siRNA to investigate whether cofilin-1 was critical for the protective effect of CsA and whether the effect of CsA on cofilin-1 was independent of its effect on synaptopodin. We found that CsA reduced proteinuria and repaired FP effacement of PAN-induced nephropathy, restored expression of nephrin, synaptopodin, cofilin-1, pho-cofilin-1 both in vivo and in vitro. CsA also repaired actin cytoskeleton impaired by PAN in vitro. The protective effect of CsA was diminished when cofilin-1 was knocked down compared to negative control. Synaptopodin knocked down had no effect on cofilin-1. The protective effect of CsA decreased significantly when cofilin-1 and synaptopodin were simultaneously knocked down compared to only cofilin-1 knock down. In conclusion, the antiproteinuric effect of CsA is derived from the stabilization of the podocyte actin cytoskeleton by upregulating expression of cofilin-1, which was independent of its effect on synaptopodin.
Unmet needs of patients with chronic obstructive pulmonary disease (COPD): a qualitative study on patients and doctors
Authors: Wong Stalia SL , Abdullah Nurdiana , Abdullah Adina , Liew Su-May , Ching Siew-Mooi , Khoo Ee-Ming , Jiwa Moyez , Chia Yook-Chin 
Published: April 2014

Abstract

Background
Chronic Obstructive Pulmonary Disease (COPD) is a chronic disease with repeated exacerbations resulting in gradual debilitation. The quality of life has been shown to be poor in patients with COPD despite efforts to improve self-management. However, the evidence on the benefit of self-management in COPD is conflicting. Whether this could be due to other unmet needs of patients have not been investigated. Therefore, we aimed to explore unmet needs of patients from both patients and doctors managing COPD.

Methods
We conducted a qualitative study with doctors and patients in Malaysia. We used convenience sampling to recruit patients until data saturation. Eighteen patients and eighteen doctors consented and were interviewed using a semi-structured interview guide. The interviews were audio-recorded, transcribed verbatim and checked by the interviewers. Data were analysed using a thematic approach.

Results
The themes were similar for both the patients and doctors. Three main themes emerged: knowledge and awareness of COPD, psychosocial and physical impact of COPD and the utility of self-management. Knowledge about COPD was generally poor. Patients were not familiar with the term chronic obstructive pulmonary disease or COPD. The word ‘asthma’ was used synonymously with COPD by both patients and doctors. Most patients experienced difficulties in their psychosocial and physical functions such as breathlessness, fear and helplessness. Most patients were not confident in self-managing their illness and prefer a more passive role with doctors directing their care.

Conclusions
In conclusion, our study showed that knowledge of COPD is generally poor. There was mislabelling of COPD as asthma by both patients and physicians. This could have resulted in the lack of understanding of treatment options, outcomes, and prognosis of COPD. The misconception that cough due to COPD was contagious, and breathlessness that resulted from COPD, had important physical and psychosocial impact, and could lead to social isolation. Most patients and physicians did not favour self-management approaches, suggesting innovations based on self-management may be of limited benefit.
Knowledge, attitudes and behaviour of hospital health-care workers regarding influenza A/H1N1: a cross sectional survey
Authors: Albano Luciana , Matuozzo Anna , Marinelli Paolo , Di Giuseppe Gabriella 
Published: April 2014

Abstract

Background
To assess the knowledge, the attitudes, and the behaviour towards influenza A/H1N1 and the vaccination among health-care workers (HCWs).

Methods
A sample of HCWs was selected from a random sample of non-teaching public hospitals, located in the cities of Naples and Avellino (Italy), received a self-administered anonymous questionnaire including questions about socio-demographic characteristics, knowledge on modes of transmission and preventative measures, attitudes and behaviour relating to influenza A/H1N1.

Results
Only 36.1% correctly knew the main modes of transmission, and that HCWs are a risk category and this level of knowledge was significantly higher in HCWs having received information through scientific journals. A higher perceived risk of contracting influenza A/H1N1 has been observed in the HCWs more knowledgeable, in those considering influenza A/H1N1 a serious disease, and in those working in surgical wards. Only 16.7% have received the influenza A/H1N1 vaccination and HCWs with more fear of contracting influenza A/H1N1, those considering vaccine more useful and less dangerous were more likely to receive vaccine.

Conclusions
Education and communication strategies for improving the level of knowledge and for the immunization uptake regarding influenza A/H1N1 HCWs are strongly needed.
Antiretroviral drug expenditure, pricing and judicial demand: an analysis of federal procurement data in Brazil from 2004–2011
Authors: Luo Jing , Oliveira Maria A , Ramos Mariana BC , Maia Aurélio , Osorio-de-Castro Claudia GS 
Published: April 2014

Abstract

Background
Previous studies have described expenditures for antiretroviral (ARV) medicines in Brazil through 2005. While prior studies examined overall expenditures, they have not have analyzed drug procurement data in order to describe the role of court litigation on access and pricing.

Methods
ARV drug procurement from private sector sources for the years 2004–2011 was obtained through the general procurement database of the Brazilian Federal Government (SIASG). Procurement was measured in Defined Daily Doses (DDD) per 1000 persons-under-treatment per day. Expenditures and price per DDD were calculated and expressed in U.S. Dollars. Justifications for ARV purchases were examined in order to determine the relationship between health litigation and incorporation into Brazil’s national treatment guidelines.

Results
Drug procurement of ARVs from private sources underwent marked expansion in 2005, peaked in 2009, and stabilized to 2008 levels by 2011. Expenditures followed procurement curves. Medications which were procured for the first time after 2007 cost more than medicines which were introduced before 2007. Judicial actions initially resulted in purchases of newer medications for a select number of patients in Brazil but ultimately expanded availability to a larger population through incorporation into the national treatment guidelines.

Conclusions
Drug procurement and expenditures for ARVs in Brazil varied between 2004–2011. The procurement of some drugs from the private sector ceased after public manufacturers started producing them locally. Judicial demand has resulted in the incorporation of newer drugs into the national treatment guidelines. In order for the AIDS treatment program to remain sustainable, efforts should be pursued to reduce prices through generic drugs, price negotiation and other public health flexibilities such as compulsory licensing.
Outage performance of relay-assisted primary and secondary transmissions in cognitive relay networks
Authors: Zhao Feng , Sun Xiangqi , Chen Hongbin , Bie Rongfang 
Published: April 2014

Abstract
In this paper, a two-phase protocol for relay-assisted spectrum sharing in cognitive relay network is proposed. The primary system comprises a transmitter-receiver pair (PT-PR) and a decode-and-forward relay (Relay), while the secondary system comprises a transmitter-receiver pair (ST-SR). In the proposed protocol, the Relay assists the transmissions of the primary users as well as the secondary users where there is no direct link between the ST and the SR. Outage probabilities of the primary system and the secondary system are derived and verified through simulations. Compared with the protocol without cooperation, better outage performance of the primary system is achieved. Meanwhile, though the ST causes interference to the PR, the interference is compensated by the cooperation of the Relay. Moreover, the proposed protocol realizes the communications of the secondary users on the condition that the secondary transmission link is not ideal.
Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton’s jelly
Authors: Amable Paola Romina , Teixeira Marcus Vinicius Telles , Carias Rosana Bizon Vieira , Granjeiro José Mauro , Borojevic Radovan 
Published: April 2014

Abstract
Introduction
Different mesenchymal stromal cells (MSC) have been successfully isolated and expanded in vitro and nowadays they are tested in clinical trials for a wide variety of diseases. Whether all MSC express the same cell surface markers or have a similar secretion profile is still controversial, making it difficult to decide which stromal cell may be better for a particular application.

Methods
We isolated human mesenchymal stromal cells from bone marrow (BM), adipose tissue (AT) and Wharton’s jelly (WJ) and cultured them in fetal bovine serum supplemented media. We evaluated proliferation, in vitro differentiation (osteogenic, adipogenic and chondrogenic potential), expression of cell surface markers and protein secretion using Luminex and ELISA assays.

Results
Cell proliferation was higher for WJ-MSC, followed by AT-MSC. Differences in surface expression markers were observed only for CD54 and CD146. WJ-MSC secreted higher concentrations of chemokines, pro-inflammatory proteins and growth factors. AT-MSC showed a better pro-angiogenic profile and secreted higher amounts of extracellular matrix components and metalloproteinases.

Conclusions
Mesenchymal stromal cells purified from different tissues have different angiogenic, inflammatory and matrix remodeling potential properties. These abilities should be further characterized in order to choose the best protocols for their therapeutic use.