bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mice with different susceptibility to tick-borne encephalitis virus infection show selective neutralizing antibody response and inflammatory reaction in the central nervous system
Authors: Palus Martin , Vojtíšková Jarmila , Salát Jiří , Kopecký Jan , Grubhoffer Libor , Lipoldová Marie , Demant Peter , Růžek Daniel 
Published: June 2013

Abstract

Background
The clinical course of tick-borne encephalitis (TBE), a disease caused by TBE virus, ranges from asymptomatic or mild influenza-like infection to severe debilitating encephalitis or encephalomyelitis. Despite the medical importance of this disease, some crucial steps in the development of encephalitis remain poorly understood. In particular, the basis of the disease severity is largely unknown.

Methods
TBE virus growth, neutralizing antibody response, key cytokine and chemokine mRNA production and changes in mRNA levels of cell surface markers of immunocompetent cells in brain were measured in mice with different susceptibilities to TBE virus infection.

Results
An animal model of TBE based on BALB/c-c-STS/A (CcS/Dem) recombinant congenic mouse strains showing different severities of the infection in relation to the host genetic background was developed. After subcutaneous inoculation of TBE virus, BALB/c mice showed medium susceptibility to the infection, STS mice were resistant, and CcS-11 mice were highly susceptible. The resistant STS mice showed lower and delayed viremia, lower virus production in the brain and low cytokine/chemokine mRNA production, but had a strong neutralizing antibody response. The most sensitive strain (CcS-11) failed in production of neutralizing antibodies, but exhibited strong cytokine/chemokine mRNA production in the brain. After intracerebral inoculation, all mouse strains were sensitive to the infection and had similar virus production in the brain, but STS mice survived significantly longer than CcS-11 mice. These two strains also differed in the expression of key cytokines/chemokines, particularly interferon gamma-induced protein 10 (IP-10/CXCL10) and monocyte chemotactic protein-1 (MCP-1/CCL2) in the brain.

Conclusions
Our data indicate that the genetic control is an important factor influencing the clinical course of TBE. High neutralizing antibody response might be crucial for preventing host fatality, but high expression of various cytokines/chemokines during TBE can mediate immunopathology and be associated with more severe course of the infection and increased fatality.
Fractionated stereotactic radiotherapy for 136 patients with locally residual nasopharyngeal carcinoma
Authors: Liu Feng , Xiao Jian-ping , Xu Guo-zhen , Gao Li , Xu Ying-jie , Zhang Ye , Jiang Xue-song , Yi Jun-lin , Luo Jing-wei , Huang Xiao-dong , Huan Fu-kui , Fang Hao , Wan Bao , Li Ye-xiong 
Published: June 2013

Abstract

Background
To evaluate the efficacy and toxicity of fractionated stereotactic radiotherapy (FSRT) in patients with residual nasopharyngeal carcinoma (NPC).

Methods
From January 2000 to December 2009, 136 NPC patients with residual lesions after primary radiotherapy (RT) were treated by FSRT. The total dose of primary RT was 68.0-78.0 Gy (median, 70.0 Gy). The median time from the primary RT to FSRT was 24.5 days. Tumor volumes for FSRT ranged from 0.60 to 77.13 cm3 (median, 13.45 cm3). The total FSRT doses were 8.0-32.0Gy (median, 19.5 Gy) with 2.0-10.0 Gy per fraction.

Results
Five-year local failure-free survival (LFFS), freedom from distant metastasis (FFDM), overall survival (OS), and disease free survival (DFS) rates for all patients were 92.5%, 77.0%, 76.2%, and 73.6%, respectively. No statistical significant differences were found in LFFS, DFS and OS in patients with stage I/II versus stage III/ IV diseases. Nineteen patients exhibited late toxicity. T stage at diagnosis was a significant prognostic factor for OS and DFS. Age was a prognostic factor for OS.

Conclusion
FSRT after external beam radiotherapy provides excellent local control for patients with residual NPC. The incidence of severe late toxicity is low and acceptable. Further investigation of optimal fractionation regimens will facilitate reduction of long-term complications.
Control of hypertension in the critically ill: a pathophysiological approach
Authors: Salgado Diamantino Ribeiro , Silva Eliezer , Vincent Jean-Louis 
Published: June 2013

AbstractSevere acute arterial hypertension can be associated with significant morbidity and mortality. After excluding a reversible etiology, choice of therapeutic intervention should be based on evaluation of a number of factors, such as age, comorbidities, and other ongoing therapies. A rational pathophysiological approach should then be applied that integrates the effects of the drug on blood volume, vascular tone, and other determinants of cardiac output. Vasodilators, calcium channel blockers, and beta-blocking agents can all decrease arterial pressure but by totally different modes of action, which may be appropriate or contraindicated in individual patients. There is no preferred agent for all situations, although some drugs may have a more attractive profile than others, with rapid onset action, short half-life, and fewer adverse reactions. In this review, we focus on the main mechanisms underlying severe hypertension in the critically ill and how using a pathophysiological approach can help the intensivist decide on treatment options.
Inhibition of pre-B cell colony-enhancing factor (PBEF/NAMPT/visfatin) decreases the ability of human neutrophils to generate reactive oxidants but does not impair bacterial killing
Authors: Roberts Kate J , Cross Andrew , Vasieva Olga , Moots Robert J , Edwards Steven W 
Published: June 2013

ABSTRACTNAMPT, also known as PBEF and visfatin, can act extracellularly as a cytokine-like molecule or intracellularly as a NAMPT, regulating NAD biosynthesis in the NAD salvage pathway. Inhibitors of NAMPT have anti-inflammatory and anticancer activity and are finding use as therapeutic agents. In view of the importance of NAD metabolism in neutrophil function, we determined the effects of NAMPT inhibition on a variety of neutrophil functions associated with their role in host protection against infections. Incubation of human neutrophils with the NAMPT inhibitor APO866 decreased neutrophil NAD(P)/H levels in a dose- and time-dependent manner but without a concomitant change in cell viability. NAMPT inhibition did not affect the expression of a number of cell-surface receptors involved in adhesion and opsono-phagocytosis, but the respiratory burst was decreased significantly. Whereas opsono-phagocytosis of Staphylococcus aureus was unaffected by NAMPT inhibition, intraphagosomal oxidant production was decreased. However, the killing efficiency of neutrophils was unaffected. These data indicate that therapeutic NAMPT inhibition is unlikely to have deleterious effects on host protection against infections, in spite of this ability to down-regulate neutrophil respiratory burst activity significantly.
Reduction of intellectual property items’ economic losses
Authors: Слезко  , Slezko Vyacheslav 
Published: June 2013

In article it is spoken about the necessity related to creation of mechanism of optimal state funding for R&amp;D. In this mechanism structure shall be included the systems of technical and economic audit of intellectual property objects. In accordance with part IV of the Russian Federation Civil code (art. 1225), it is possible to obtain the following two groups of intellectual property items as a result of R&amp;D on the area of information technologies: the items of patent law and copyright law. It is possible to carry out the information technologies’ technical audit with application of COBIT [Control Objectives for Information and related Technology] and PCI DSS [Payment Card Industry Data Security Standard] standards.
Economic audit during R&amp;D in the area of information resources shall include the carrying out expert examinations on the costs of research and assessment of intellectual property items’ market value. All this shall lead to significant reduction of economic losses in the process of state financing related to research and development.
Changes in tibial bone density measured from standard radiographs in cemented and uncemented total knee replacements after ten years’ follow-up
Authors: Small S. R. , Ritter M. A. , Merchun J. G. , Davis K. E. , Rogge R. D. 
Published: June 2013

Stress shielding resulting in diminished bone density following total knee replacement (TKR) may increase the risk of migration and loosening of the prosthesis. This retrospective study was designed to quantify the effects of the method of fixation on peri-prosthetic tibial bone density beneath cemented and uncemented tibial components of similar design and with similar long-term survival rates. Standard radiographs taken between two months and 15 years post-operatively were digitised from a matched group of TKRs using cemented (n = 67) and uncemented (n = 67) AGC tibial prostheses. Digital radiograph densitometry was used to quantify changes in bone density over time. Age, length of follow-up, gender, body mass index and alignment each significantly influenced the long-term pattern of peri-prosthetic bone density. Similar long-term changes in density irrespective of the method of fixation correlated well with the high rate of survival of this TKR at 20 years, and suggest that cemented and uncemented fixation are both equally viable.Cite this article: Bone Joint J 2013;95-B:911–16.
Prolonged incubation time does not increase sensitivity for the diagnosis of implant-related infection using samples prepared by sonication of the implants
Authors: Esteban J. , Alvarez-Alvarez B. , Blanco A. , Fernández-Roblas R. , Gadea I. , Garcia-Cañete J. , Sandoval E. , Valdazo M. 
Published: June 2013

We have designed a prospective study to evaluate the usefulness of prolonged incubation of cultures from sonicated orthopaedic implants. During the study period 124 implants from 113 patients were processed (22 osteosynthetic implants, 46 hip prostheses, 54 knee prostheses, and two shoulder prostheses). Of these, 70 patients had clinical infection; 32 had received antibiotics at least seven days before removal of the implant. A total of 54 patients had sonicated samples that produced positive cultures (including four patients without infection). All of them were positive in the first seven days of incubation. No differences were found regarding previous antibiotic treatment when analysing colony counts or days of incubation in the case of a positive result. In our experience, extending incubation of the samples to 14 days does not add more positive results for sonicated orthopaedic implants (hip and knee prosthesis and osteosynthesis implants) compared with a conventional seven-day incubation period. Cite this article: Bone Joint J 2013;95-B:1001–6.
Improvement in quality of life after arthroscopic capsular release for contracture of the shoulder
Authors: Dattani R. , Ramasamy V. , Parker R. , Patel V. R. 
Published: June 2013

There is little published information on the health impact of frozen shoulder. The purpose of this study was to assess the functional and health-related quality of life outcomes following arthroscopic capsular release (ACR) for contracture of the shoulder. Between January 2010 and January 2012 all patients who had failed non-operative treatment including anti-inflammatory medication, physiotherapy and glenohumeral joint injections for contracture of the shoulder and who subsequently underwent an ACR were enrolled in the study. A total of 100 patients were eligible; 68 underwent ACR alone and 32 had ACR with a subacromial decompression (ASD). ACR resulted in a highly significant improvement in the range of movement and functional outcome, as measured by the Oxford shoulder score and EuroQol EQ-5D index. The mean cost of a quality-adjusted life year (QALY) for an ACR and ACR with an ASD was £2563 and £3189, respectively. ACR is thus a cost-effective procedure that can restore relatively normal function and health-related quality of life in most patients with a contracture of the shoulder within six months after surgery; and the beneficial effects are not related to the duration of the presenting symptoms. Cite this article: Bone Joint J 2013;95-B:942–6.
Yeast Proteins Originated from the Production of Brazilian Bioethanol Quantification and Content
Authors: Steckelberg Claudia , Andrietta Maria da GraÇa Stupiello , Andrietta Silvio Roberto , Stupielloé Erika Nogueira Andrade 
Published: June 2013

Abstract
The purpose of this work was to determine the levels of protein and the amino acid distribution in the cell mass of yeast strains (Saccharomyces sensu stricto) originated from Brazilian bioethanol industries. The protein was analyzed with the Kjeldahl method and the amino acids, by using high-performance liquid chromatography (HPLC). The percentages of the protein found ranged from 39 to 49%. The results show that in spite of some variation in numbers between the different yeast strains, all of them presented an amino acid profile similar to the one in the literature for S. cerevisae. The amino acids that have occurred in the largest amounts were: aspartic, glutamic acids and lysine, and those in the lowest amounts were: cysteine and methionine. Although the characteristics of the feedstock used and the process conditions are determinant of the protein values obtained in dry mass, this work elucidates that the intrinsic properties of the yeast strain influence these values.
Laser-based non-invasive spectrophotometry – An overview of possible medical applications
Authors: Rogatkin Dmitry , Shumskiy Vyacheslav , Tereshenko Sergey , Polyakov Pavel 
Published: June 2013

AbstractThe problem of the medical application of non-invasive (The specially devised multifunctional laser diagnostic system “LAKK-M” was used as a diagnostic instrument in the majority of studies in MONIKI, allowing a combination of methods, such as non-invasive medical spectrophotometry in the form of laser fluorescence spectroscopy, laser Doppler flowmetry, tissue reflectance oximetry, etc. The system was used in both the experiments on laboratory animals, and for treatment of patients in different clinics of the institute.Within the last decade, extensive scientific data has been obtained which has opened up the possibility of using non-invasive medical spectrophotometryMethods of complex non-invasive medical spectrophotometry appear to be an efficient tool in practical medicine for differential diagnostics of a number of diseases and pathologies, as well as for monitoring and prediction of the treatment outcome. At the same time, they also create an important perspective for gaining novel and fundamental knowledge about the blood microcirculation system which was not available earlier due to the absence of the diagnostic technology allowing information to be obtained
Evaluation of a Variable‐Thread Tapered Implant in Extraction Sites With Immediate Temporization: A 3‐Year Multicenter Clinical Study
Authors: Kolinski Martin L. , Cherry James E. , McAllister Bradley S. , Parrish Kenneth D. , Pumphrey David W. , Schroering Robert L. 
Published: June 2013

Background: The purpose of this clinical study is to evaluate the radiographic bone remodeling, survival rate, and soft tissue health surrounding a variable‐thread tapered implant immediately placed in extraction sites.Methods: Sixty implants were placed in 55 patients at six centers according to a predetermined protocol. All implants were placed in extraction sockets and were subjected to immediate temporization and function. Definitive prostheses (58 single crowns and one two‐unit fixed bridge) were placed within the first year. Clinical and radiographic examinations were performed at implant placement and after 3, 6, 12, 24, and 36 months. Measurements of implant stability, papilla index, plaque, peri‐implant mucosa, and marginal bone levels were recorded at each visit.Results: Thirty‐five implants were evaluated at both implant insertion and 3‐year follow‐up. Bone levels were observed at 6 months after surgery and yearly intervals thereafter and remained stable throughout the study. There was a slight decrease in mean bone level from −0.68 mm at implant insertion to −0.93 mm at the 6‐month recall and then an increase of bone to −0.53 mm from the reference point at the 2‐year follow‐up (an average increase of 0.15 mm from implant insertion). Bone levels remained steady between the 2‐year recall and the 3‐year recall. Papilla scores increased significantly (P <0.001; Wilcoxon signed‐rank test) from insertion to the 3‐year follow‐up, with most of the increase occurring during the first year. Patient assessments of function, esthetics, feel of implant, speech, and self‐esteem also showed significant improvement over the course of the study.Conclusions: The results, over 36 months, indicate that the variable‐thread tapered implant can be used safely and effectively under demanding conditions as an immediate postextraction tooth replacement. Bone remodeling remained stable with a slight increase, and patients expressed high levels of satisfaction with the restorative results over the course of the study.
The Effect of α‐Tocopherol and Selenium on Human Gingival Fibroblasts and Periodontal Ligament Fibroblasts In Vitro
Authors: Nizam Nejat , Discioglu Feridun , Saygun Isil , Bal Vehbi , Avcu Ferit , Ozkan Cansel Kose , Serdar Muhittin Abdulkadir 
Published: June 2013

Background: The aim of the present study is to evaluate the effect of α‐tocopherol and selenium on gingival fibroblasts (GFs) and periodontal ligament fibroblasts (PDLFs) in terms of proliferation, basic fibroblast growth factor (bFGF) release, collagen type I synthesis, and wound healing.Methods: Primary cultures of human GFs and PDLFs were isolated. Four test groups and a control group free of medication was formed. In group E, 60 μM α‐tocopherol was used, and in groups ES1, ES2, and ES3, the combination of 60 μM α‐tocopherol with 5 × 10−9 M, 10 × 10−9 M, and 50 × 10−9 M selenium was used, respectively. Viability, proliferation, bFGF, and collagen type I synthesis from both cell types were evaluated at 24, 48, and 72 hours, and healing was compared on a new wound‐healing model at 12, 24, 36, 48, and 72 hours.Results: α‐Tocopherol alone significantly increased the healing rate of PDLFs at 12 hours and increased bFGF and collagen type I release from GFs and PDLFs at 24, 48, and 72 hours. The α‐tocopherol/selenium combination significantly enhanced the proliferation rate of both cells at 48 hours, decreased the proliferation of PDLFs at 72 hours, and increased the healing rate of GFs at 12 hours and PDLFs at 12 and 48 hours. bFGF and collagen type I synthesis was also increased in both cell types at 24, 48, and 72 hours by α‐tocopherol/selenium combination.Conclusion: α‐Tocopherol and α‐tocopherol/selenium combination is able to accelerate the proliferation rate and wound‐healing process and increase the synthesis of bFGF and collagen type I from both GFs and PDLFs.
Sell Spot or Sell Forward? Analysis of Oil-Trading Decisions With the Two-Factor Price Model and Simulation
Authors: Jafarizadeh Babak , Bratvold Reidar B. 
Published: June 2013

SummaryIn oil and gas markets, the relationships between the spot and futures prices reveal important opportunities for value creation. When oil prices are in contango (i.e., when futures prices are higher than the expected future spot prices), it may be profitable for a trader to hold oil in storage and enter into a futures contract instead of selling oil in the spot market. The decision to either sell oil in the spot market or use the storage to sell oil in the future is usually challenging because the future spot prices and futures prices are uncertain. In this paper, we discuss the storage trading decisions by use of a realistic example, and we propose an analysis methodology on the basis of a two-factor price process for modeling spot and futures oil prices. The dynamic decision problem, sell spot or sell forward, is analyzed with a forward dynamic optimization algorithm and the least-squares Monte Carlo simulation.
Non-hyperbolicity in random regular graphs and their traffic characteristics
Authors: Tucci Gabriel 
Published: June 2013

Abstract
In this paper we prove that random d-regular graphs with d ≥ 3 have traffic congestion of the order O(n logd−13
n) where n is the number of nodes and geodesic routing is used. We also show that these graphs are not asymptotically δ-hyperbolic for any non-negative δ almost surely as n → ∞.
The generalized Laguerre inequalities and functions in the Laguerre-Pólya class
Authors: Csordas George , Vishnyakova Anna 
Published: June 2013

Abstract
The principal goal of this paper is to show that the various sufficient conditions for a real entire function, φ(x), to belong to the Laguerre-Pólya class (Definition 1.1), expressed in terms of Laguerre-type inequalities, do not require the a priori assumptions about the order and type of φ(x). The proof of the main theorem (Theorem 2.3) involving the generalized real Laguerre inequalities, is based on a beautiful geometric result, the Borel-Carathédodory Inequality (Theorem 2.1), and on a deep theorem of Lindelöf (Theorem 2.2). In case of the complex Laguerre inequalities (Theorem 3.2), the proof is sketched for it requires a slightly more delicate analysis. Section 3 concludes with some other cognate results, an open problem and a conjecture which is based on Cardon’s recent, ingenious extension of the Laguerre-type inequalities.
Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania
Authors: Patamsytė Jolanta , Rančelis Vytautas , Čėsnienė Tatjana , Kleizaitė Violeta , Tunaitienė Virginija , Naugžemys Donatas , Vaitkūnienė Virginija , Žvingila Donatas 
Published: June 2013

Abstract
The alien species Erigeron annuus (L.) Pers. is in an intensive spreading phase in Lithuania. Random amplified polymorphic DNA (RAPDs) and inter-simple sequence repeats (ISSRs) assays were used to study the genetic structure of old and new invasive populations and to determine the most spread genotypes of this species in Lithuania. Pairwise genetic distances between populations established using RAPD and ISSR markers significantly correlated (r=0.91, P<0.05). Our study indicates that there are two genetically different types of E. annuus populations. The first type is represented by a widely spread main clone and related monomorphic populations. The second type is represented by polymorphic populations, some of them present at sites where E. annuus has not been previously observed. Main clone predominates in nine populations and is from the region where this species was first described in natural ecosystems of Lithuania. UPGMA cluster analysis revealed genetic relationships between the main clone and accessions from old cemeteries where E. annuus has been grown as an ornamental plant. We found high genetic differentiation among populations (G
ST=0.58 for RAPDs, G
ST=0.64 for ISSRs). Taken together, our results will contribute to the monitoring of E. annuus spread in Lithuania.
Invariance of the Bajraktarević means with respect to the Beckenbach-Gini means
Authors: Matkowski Janusz 
Published: June 2013

Abstract
Assuming that the involved functions are three times differentiable, we determine all Bajraktarević means which are invariant respect to the mean-type mapping of the Beckenbach-Gini mean type. Some applications in iteration theory and functional equation are presented.
On the quadratic convergence of Newton’s method under center-Lipschitz but not necessarily Lipschitz hypotheses
Authors: Argyros Ioannis , Hilout Saïd 
Published: June 2013

Abstract
We provide new local and semilocal convergence results for Newton’s method in a Banach space. The sufficient convergence conditions do not include the Lipschitz constant usually associated with Newton’s method. Numerical examples demonstrating the expansion of Newton’s method are also provided in this study.