bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analysis and single-cell analysis reveals FAM110B as a potential target for survival and immunotherapy
Authors: Li Yuwei , Li Xiaoxi , Wu Bihua , Su Shuangyan , Su Yunpeng , Guo Le 
Published: August 2024

Background: FAM110B belongs to the family that has a 110 sequence similarity (FAM110) and is located in the centrosome and mitotic spindle. FAM110B has been linked to tumor cell growth in earlier research. Uncertainty exists regarding FAM110B’s function within the tumor microenvironment is unclear as well as pan-cancer.Methods: In order to assess the variation in FAM110B expression within normal and pan-cancer tissues, we combined the TCGA and GTEx databases. The cBioPortal database and the GSCALite platform were used to examine the variation in genome and methylation alteration of FAM110B. Cox regression, Kaplan-Meier, and SangerBox were employed to examine the clinical features and prognosis of FAM110B and pan-cancer. The purpose of the correlational research was to investigate the associations within immunerelated genes, tumor mutation burden, microsatellite instability, immune-related genes, and immunological checkpoints and FAM110B expression. ESTIMATE, EPIC, QUANTISEQ, and MCPCOUNTER methods were used to calculate the interaction among FAM110B expression as well as the tumor immune microenvironment. The immunoinfiltration and function of FAM110B were analyzed by single-cell databases (TISCH and CancerSEA). Finally, we evaluated the sensitivity of FAM110B to small-molecule medications through GDSC and CTRP databases.Results: The transcription and protein expression of FAM110B varies significantly throughout cancer types, and this has predictive value for the prognosis of some tumors; including brain lower grade glioma (LGG), stomach adenocarcinoma (STAD), pancreatic adenocarcinoma (PAAD), etc. In the tumor microenvironment, the expression level of FAM110B was associated with immune cell infiltration, immune checkpoint immune regulatory genes, tumor mutational burden, and microsatellite fragility to a certain extent.Conclusion: This work investigates the possibility of utility of FAM110B as a marker to forecast pan-cancer immunotherapy response, providing a theoretical basis for cancer therapy.
Discovery of an 8-oxoguanine regulator PCBP1 inhibitor by virtual screening and its synergistic effects with ROS-modulating agents in pancreatic cancer
Authors: Qiao Kexiong , Xu Chengjie , Zhang Chaolei , Wang Qianqian , Jiang Jun , Chen Zongrong , Zhou Liangjing , Jia Shengnan , Cao Liping 
Published: August 2024

Introduction: Drugs that target reactive oxygen species (ROS) metabolism have progressed the treatment of pancreatic cancer treatment, yet their efficacy remains poor because of the adaptation of cancer cells to high concentration of ROS. Cells cope with ROS by recognizing 8-oxoguanine residues and processing severely oxidized RNA, which make it feasible to improve the efficacy of ROS-modulating drugs in pancreatic cancer by targeting 8-oxoguanine regulators.Methods: Poly(rC)-binding protein 1 (PCBP1) was identified as a potential oncogene in pancreatic cancer through datasets of The Cancer Genome Atlas (TCGA) project and Gene Expression Omnibus (GEO). High-throughput virtual screening was used to screen out potential inhibitors for PCBP1. Computational molecular dynamics simulations was used to verify the stable interaction between the two compounds and PCBP1 and their structure–activity relationships. In vitro experiments were performed for functional validation of silychristin.Results: In this study, we identified PCBP1 as a potential oncogene in pancreatic cancer. By applying high-throughput virtual screening, we identified Compound 102 and Compound 934 (silychristin) as potential PCBP1 inhibitors. Computational molecular dynamics simulations and virtual alanine mutagenesis verified the structure–activity correlation between PCBP1 and the two identified compounds. These two compounds interfere with the PCBP1–RNA interaction and impair the ability of PCBP1 to process RNA, leading to intracellular R loop accumulation. Compound 934 synergized with ROS agent hydrogen peroxide to strongly improve induced cell death in pancreatic cancer cells.Discussion: Our results provide valuable insights into the development of drugs that target PCBP1 and identified promising synergistic agents for ROS-modulating drugs in pancreatic cancer.
Longitudinal assessment of glymphatic changes following mild traumatic brain injury: Insights from perivascular space burden and DTI-ALPS imaging
Authors: Zhuo Jiachen , Raghavan Prashant , Li Jiang , Roys Steven , Njonkou Tchoquessi Rosy Linda , Chen Hegang , Wickwire Emerson M. , Parikh Gunjan Y. , Schwartzbauer Gary T. , Grattan Lynn M. , Wang Ze , Gullapalli Rao P. , Badjatia Neeraj 
Published: August 2024

IntroductionTraumatic brain injury (TBI) even in the mild form may result in long-lasting post-concussion symptoms. TBI is also a known risk to late-life neurodegeneration. Recent studies suggest that dysfunction in the glymphatic system, responsible for clearing protein waste from the brain, may play a pivotal role in the development of dementia following TBI. Given the diverse nature of TBI, longitudinal investigations are essential to comprehending the dynamic changes in the glymphatic system and its implications for recovery.MethodsIn this prospective study, we evaluated two promising glymphatic imaging markers, namely the enlarged perivascular space (ePVS) burden and Diffusion Tensor Imaging-based ALPS index, in 44 patients with mTBI at two early post-injury time points: approximately 14 days (14Day) and 6–12 months (6–12Mon) post-injury, while also examining their associations with post-concussion symptoms. Additionally, 37 controls, comprising both orthopedic patients and healthy individuals, were included for comparative analysis.ResultsOur key findings include: (1) White matter ePVS burden (WM-ePVS) and ALPS index exhibit significant correlations with age. (2) Elevated WM-ePVS burden in acute mTBI (14Day) is significantly linked to a higher number of post-concussion symptoms, particularly memory problems. (3) The increase in the ALPS index from acute (14Day) to the chronic (6–12Mon) phases in mTBI patients correlates with improvement in sleep measures. Furthermore, incorporating WM-ePVS burden and the ALPS index from acute phase enhances the prediction of chronic memory problems beyond socio-demographic and basic clinical information.ConclusionePVS burden and ALPS index offers distinct values in assessing glymphatic structure and activity. Early evaluation of glymphatic function could be crucial for understanding TBI recovery and developing targeted interventions to improve patient outcomes.
Examining child schooling/care location and child temperament as predictors of restaurant-related behaviors during the COVID-19 pandemic: findings from a nationally representative survey
Authors: Goldsmith Juliana , Ferrante Mackenzie J. , Tauriello Sara , Epstein Leonard H. , Leone Lucia A. , Anzman-Frasca Stephanie 
Published: August 2024

PurposeEmerging research highlights impacts of the COVID-19 pandemic on U.S. families, including changes in eating behavior and increased child body mass index. Aims of the present study were to examine whether child temperament and at-home vs. out-of-home childcare/school predicted families’ restaurant-related behaviors during the pandemic. Examining energy balance-related behaviors, like restaurant patronage, during the pandemic can help better understand lasting impacts on child health behaviors and health outcomes.MethodsAn online survey was administered to U.S. parents with a 4-to-8-year-old child in October 2020 (n = 1,000). Linear and logistic regression examined whether child temperament and at home vs. out-of-home childcare/school predicted: (1) the frequency the child consumed restaurant meals (take-out, delivery, dine-in), (2) who chose the child’s restaurant meal, and (3) parent-reported reasons for the child’s meal choice. Income, education, employment, race/ethnicity, and regional COVID-19 restrictions were tested as covariates.ResultsParents with children higher on negative affectivity reported more frequent restaurant use in-person (p &lt; 0.05) and via delivery (p &lt; 0.05) compared to parents of children lower on negativity. Child negativity was also linked with parent-reported reasons for children’s restaurant meal choices. Parents of children receiving at-home childcare/schooling used delivery services less frequently than those receiving out-of-home care or schooling (p &lt; 0.01).ConclusionThese findings suggest that individual and family factors may impact restaurant use and the meal selection process for children using restaurants during and beyond the COVID-19 era. Continued examination of individual differences in the impacts of the COVID-19 pandemic can facilitate intervention and policy approaches that fit with different families’ needs.
Diagnostic and prognostic significance of ALU-based cell-free DNA in colorectal cancer: a systematic review and meta-analysis
Authors: Taghizadeh-Teymorloei Mohammad , Alizadeh Leila , Matin Somaieh , Jafari-Koshki Tohid , Karimi Abbas 
Published: August 2024

IntroductionColorectal cancer (CRC) is a major global health concern. This study aimed to investigate the role of ALU-based cell-free DNA (cfDNA) in the diagnosis and prognosis of CRC.MethodsWe selected relevant literature from PubMed, Scopus, Web of Science, EMBASE, and Science Direct databases based on strict inclusion and exclusion criteria. 17 eligible studies were included in the final analysis (13 studies for diagnostic and 4 studies for prognostic meta-analysis). The search covered relevant publications up to July 1, 2024.ResultsThe pooled sensitivity, specificity, and diagnostic odds ratios (DOR) of ALU-based cfDNA in CRC diagnosis were 0.81 (95% CI= [0.70, 0.89]), 0.90 (95% CI= [0.70, 0.96]), and 40.58 (95% CI= [17.87, 92.19]), respectively. The area under the ROC curve was 0.92 (95% CI= [0.89, 0.94]). Patients with higher concentrations of plasma/serum ALU-based cfDNA had poorer overall survival (OS) (pooled hazard ratio = 2.33 ([95% CI= [1.80, 3.03]).ConclusionThe current evidence supports the utility of circulating ALU as a promising non-invasive diagnostic and prognostic tool for CRC. Furthermore, as a potential biomarker, ALU-based cfDNA could play a significant role in clinical application.Clinical implicationsThe evidence suggests that circulating ALU-based cell-free DNA (cfDNA) holds promise as a non-invasive diagnostic and prognostic tool for colorectal cancer, potentially enhancing clinical decision-making.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier PROSPERO (CRD42023486369).
External breast prostheses after mastectomy: production and selection of a low-cost functional model to be performed in developing countries
Authors: Vieira René Aloisio da Costa , Ibrahim Matheus Sampaio , de Souza Araujo Lucas Guimarães , Souza Junior José de Assis e , de Freitas Carla Simone Moreira , de Oliveira Luiz Carlos Navarro 
Published: August 2024

IntroductionBreast cancer is one of the most common types of cancer affecting women. Despite advancements in early diagnosis, neoadjuvant therapy, and various treatment modalities, mastectomy remains a common procedure for many women. Although some women opt for reconstructive surgery (BR), many do not have the indication, desire, or opportunity to undergo this procedure.MethodsAn easily manufactured, washable, lightweight, and inexpensive external breast prosthesis (EBP) model was developed specifically for the study. Participants were presented with five EBP models–one commercially available, three manufactured options, and one created for the study–and were asked to choose a prosthesis. We also evaluated the factors associated with non-adherence to EBP among women who had undergone mastectomy without BR. The chi-square test was used to assess adherence or non-adherence to EBP, while logistic regression was used to identify factors associated with non-adherence.ResultsWe introduced a low-cost, lightweight, washable EBP model. When participants were asked to choose between two prostheses, the silicone prosthesis was the first choice for 33.9% of the participants, while the prosthesis created for the study emerged as the second choice for 70.5%. Out of the 72 women assessed, 45.8% (33) opted not to use any of the EBP models. Our analysis revealed that age and BMI were significantly associated with non-use of EBP.ConclusionMultiple barriers contribute to non-adherence to EBP, underscoring the need for interventions aimed at improving patient knowledge and adherence. This study introduces a lightweight, easily reproducible, and low-cost EBP model.
Alchemilla vulgaris modulates isoproterenol-induced cardiotoxicity: interplay of oxidative stress, inflammation, autophagy, and apoptosis
Authors: Anajirih Nuha , Abdeen Ahmed , Taher Ehab S. , Abdelkader Afaf , Abd-Ellatieff Hoda A. , Gewaily Mahmoud S. , Ahmed Nashwa E. , Al-Serwi Rasha H. , Sorour Safwa M. , Abdelkareem Heba M. , Ebrahim Elturabi , El-Sherbiny Mohamed , Imbrea Florin , Imbrea Ilinca , Ramadan Mahmoud M. , Habotta Ola A. 
Published: August 2024

Introduction: Isoproterenol (ISO) is regarded as an adrenergic non-selective β agonist. It regulates myocardial contractility and may cause damage to cardiac tissues. Alchemilla vulgaris (AV) is an herbal plant that has garnered considerable attention due to its anti-inflammatory and antioxidant bioactive components. The present investigation assessed the cardioprotective potential of AV towards ISO-induced myocardial damage.Methods: Four groups of mice were utilized: control that received saline, an ISO group (85 mg/kg, S.C.), ISO + AV100, and ISO + AV200 groups (mice received 100 or 200 mg/kg AV orally along with ISO).Results and discussion: ISO induced notable cardiac damage demonstrated by clear histopathological disruption and alterations in biochemical parameters. Intriguingly, AV treatment mitigates ISO provoked oxidative stress elucidated by a substantial enhancement in superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) content, as well as a considerable reduction in malondialdehyde (MDA) concentrations. In addition, notable downregulation of inflammatory biomarkers (IL-1β, TNF-α, and RAGE) and the NF-κB/p65 pathway was observed in ISO-exposed animals following AV treatment. Furthermore, the pro-apoptotic marker Bax was downregulated together with autophagy markers Beclin1 and LC3 with in ISO-exposed animals when treated with AV. Pre-treatment with AV significantly alleviated ISO-induced cardiac damage in a dose related manner, possibly due to their antioxidant and anti-inflammatory properties. Interestingly, when AV was given at higher doses, a remarkable restoration of ISO-induced cardiac injury was revealed.
The application of rhubarb concoctions in traditional Chinese medicine and its compounds, processing methods, pharmacology, toxicology and clinical research
Authors: Wen Yi , Yan Pei-Jia , Fan Pei-Xuan , Lu Shan-Shan , Li Mao-Ya , Fu Xian-Yun , Wei Shao-Bin 
Published: August 2024

ObjectiveThis study reviews the development of rhubarb processing and the current status of pharmacological research. We summarized the effects of different processing methods on the active compounds, pharmacological effects, and toxicity of rhubarb, as well as the clinical application of different concoctions, providing reference for further pharmacological research and clinical application of rhubarb.MethodsA comprehensive literature review was conducted using databases such as Pubmed, Embase, National Science and Technology Library, Web of science, CNKI, China Science and Technology Journal Database, SinoMed, and the Pharmacopoeia of the People’s Republic of China. Search terms included “rhubarb”, “raw rhubarb”, “wine rhubarb”, “cooked rhubarb”, “rhubarb charcoal”, “herbal processing”, “compounds”, “pharmacological effects”, “inflammation”, “gastrointestinal bleeding”, and “tumor”.ResultsHistorical records of rhubarb processing date back to the Han Dynasty, with continual innovations. Currently, the types of rhubarb used in traditional Chinese medicine have stabilized to three species: Rheum palmatum L., Rheum tanguticum Maxim.ex Balf. and Rheum officinale Baill. Common concoctions include raw rhubarb, wine rhubarb, cooked rhubarb and rhubarb charcoal. The active compounds of rhubarb are known to defecation, exhibit antibacterial and anti-inflammatory properties, regulate coagulation, protect the digestive system, and possess anti-tumor activities. Guided by Chinese medicine theory, the use of different rhubarb concoctions can enhance specific effects such as purgation to eliminate accumulation, clearing heat and toxins, cooling blood to stop hemorrhages, activating blood circulation to remove blood stasis, and inducing dampness to descend jaundice, thereby effectively treating various diseases. The therapeutic impact of these concoctions on diseases reflects not only in the changes to the active compounds of rhubarb but also in the formulations of traditional Chinese medicine. Processing has also shown advantages in reducing toxicity.ConclusionDifferent processing methods alter the active compounds of rhubarb, thereby enhancing its various pharmacological effects and meeting the therapeutic needs of diverse diseases. Selecting an appropriate processing method based on the patient’s specific conditions can maximize its pharmacological properties and improve clinical outcomes.
Shallow tillage mitigates plant competition by increasing diversity and altering plant community assembly process
Authors: Li Zihao , Qiao Jirong , Li Zhuofan , Gao Xiaowei , Hong Guangyu , Yang Haifeng , Hu Ercha , Liu Chenming , Wang Xiaojiang , Liu Guanzhi , Gao Runhong 
Published: August 2024

IntroductionUnderstanding how human activities affect biodiversity is needed to inform systemic policies and targets for achieving sustainable development goals. Shallow tillage to remove Artemisia ordosica is commonly conducted in the Mu Us Desert. However, the impacts of shallow tillage on plant community species diversity, phylogenetic structure, and community assembly processes remain poorly understood.MethodsThis study explores the effects of shallow tillage on species diversity including three a-diversity and two b-diversity indicators, as well as phylogenetic structure [phylogenetic diversity (PD), net relatedness index (NRI), and nearest taxon index (NTI)]. Additionally, this research analyzes the effects of shallow tillage on the community assembly process.Results and discussionThe results showed that the a-diversity index, b-diversity index, and PD of the shallow tillage (ST) communities were significantly higher than those of the non-shallow tillage (NT) communities, and the phylogenetic structures of both the ST and NT communities tended to be differentiated, with competitive exclusion being the main mechanism of plant assembly. However, shallow tillage increased the relative importance of the stochastic processes dominated by dispersal limitation, mitigating plant competition in the communities. This conclusion was supported by the Raup–Crick difference index-based analysis.ConclusionTherefore, for the ecological restoration of the Mu Us Desert, species with adaptability and low niche overlap should be selected to increase the utilization efficiency of the environmental resources. The results of this study provide a foundation for policy development for ecosystem management and restoration in the Mu Us Desert.
The work stress, occupational burnout, coping strategies and organizational support of elite sports coaches in Sichuan Province: the mediating role of organizational support
Authors: Yu Liang , Cheng Liang 
Published: August 2024

ObjectiveThis study investigated the relationships between job pressure, occupational burnout, organizational support and coping strategies among competitive sports coaches in Sichuan Province, China. It also assesses the impact of job pressure on occupational burnout and the mediating roles of organizational support and coping strategies.MethodsA survey was conducted with 207 competitive sports coaches from Sichuan Province, China. Basic information and data on job pressure, occupational burnout, organizational support and coping strategies were collected. Correlations between variables were analyzed, and a theoretical model for hypothesizing the mediating effects was established. A multiple regression model was used to predict the relationships between occupational burnout and job pressure, organizational support and coping strategies.Results(1) Job pressure was significantly positively correlated with occupational burnout (R = 0.436, p &lt; 0.001) and negative coping (R = 0.389, p &lt; 0.001) but significantly negatively correlated with organizational support (R = −0.503, p &lt; 0.001). Occupational burnout was significantly negatively correlated with academic title (R = −0.158, p = 0.023) and organizational support (R = −0.349, p &lt; 0.001) but significantly positively correlated with negative coping (R = 0.440, p &lt; 0.001). Organisational support was significantly positively correlated with positive coping (R = 0.222, p = 0.001) but significantly negatively correlated with negative coping (R = −0.207, p = 0.003). (2) Mediating effects: Job pressure indirectly affects occupational burnout via organizational support and negative coping strategies. (3) Multiple regression: Y1 (Job Pressure) = 69.262 + 1.172 × Emotional Exhaustion −2.231 × Emotional Support +1.041 × Negative Coping −6.554 × Academic Title (from high to low). Y2 (Occupational Burnout) = 25.609 + 0.141 × Job Pressure − 0.306 × Organisational Support +0.620 × Negative Coping −2.097 × Academic Title.ConclusionJob pressure is a significant factor leading to occupational burnout among competitive sports coaches in Sichuan Province, China, and factors such as role, interpersonal relationships and career development are closely related to occupational burnout. The sense of organizational support and negative coping strategies play a mediating role between job pressure and occupational burnout. Reducing emotional exhaustion and negative coping, enhancing emotional support and improving the academic titles of coaches can help to reduce job pressure and occupational burnout among competitive sports coaches in Sichuan Province.