bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring Socio-demographic and Geographical Variation of Adults Hypertension in Bangladesh: Spatial Hotspot Analysis
Authors: Tabashsum Anika , Mahdee Chowdhury Mashrur , Asha Most. Jannatul Ferdous , Khan Md. Ashraful Islam , Rahman Md. Aminur 
Published: July 2024

AbstractHypertension is a chronic medical condition where blood pressure is too high. In Bangladesh, the overall incidence of hypertension is rising, like in other developing countries. Recent studies show hypertension can cause other non-communicable diseases such as cardiovascular diseases, strokes, etc. Utilizing information from the Bangladesh Demographic and Health Survey (BDHS) 2017–18, a cross-sectional and spatial analysis was done to determine the prevalence of hypertension. The existence of spatial autocorrelation is evaluated by using Moran’s I statistic, hotspot analysis, cold spot analysis, and influential observation analysis. The study was conducted on the basis of different factors such as gender, place of residence, age, BMI, etc. Among 12,926 people (43.19% men, 56.81% women), the women had a higher total prevalence of hypertension (41.18% (95% CI, 41.35–45.67) compared to the men’s 58.82% (95% CI, 57.16–60.45). Out of the total number of observations, 27.47% had hypertension. Out of all hypertensive people, 57.56% are unaware of their condition, which is very concerning. The study also finds that 28.4% are from urban areas and 71.6% are from rural areas. People living in the Dhaka division had a higher prevalence of hypertension (20.5%), and those in Sylhet had the lowest prevalence of hypertension (6.2%). There also exists a statistically relevant spatial autocorrelation (hypertension: Moran’s I index = 0.27, p<0.001). These are the spatial clustering maps, and according to the LISA cluster map, the Northeast districts are the cold spots (8), while the hotspots (4) are Bagerhat, Rajbari, Pirojpur, and Munshiganj. Approximately more than one-fourth of the adult population of Bangladesh had hypertension. The analysis discovered the hotspots and cold spots of the prevalence of hypertension. These findings could facilitate awareness and treatment of hypertension and therefore serve as support for researchers and policymakers.
Ixodes ricinusbites promote allergic skin inflammation and intestinal tuft and mast cell expansion in mice
Authors: Leyva-Castillo Juan Manuel , Strakosha Maria , Smith Sophia E.M. , Vega-Mendoza Daniela , Elkins Megan , Chou Janet , Vogel Peter , Boulanger Nathalie 
Published: July 2024

ABSTRACTBackgroundTick bites often promote local allergic reactions in the skin and predispose to red meat allergy. The mechanisms involved in these processes are not fully understood. Here we investigated the local changes to the skin and intestine induced by tick bites.MethodsC3H/HEN or Balb/c mice were subjected to either tick bites byIxodes ricinus(I. ricinus) or mechanical skin injury. Skin or intestine was analyzed a different time point by transcriptomic and histological techniques.ResultsOur results indicate thatI. ricinusbites promote epidermal hyperplasia, spongiosis and an accumulation of eosinophils and mast cells in the bitten skin. In addition,I. ricinusbites promote the expression of genes and activate pathways also induced by mechanical skin injury elicited by tape stripping. Remarkably, similar to tape stripping,I. ricinusbites promote an increase in total serum IgE, and intestinal tuft cell and mast cell expansion.ConclusionI. ricinusbites in mice promote cutaneous inflammation that resembles allergic skin inflammation, as well as intestinal changes that could play a role in the predisposition to red meat allergy.
Visualizing the translation landscape in human cells at high resolution
Authors: Zheng Wei , Zhang Yuekang , Wang Jimin , Wang Shuhui , Chai Pengxin , Bailey Elizabeth J , Guo Wangbiao , Devarkar Swapnil C , Wu Shenping , Lin Jianfeng , Zhang Kai , Liu Jun , Lomakin Ivan B , Xiong Yong 
Published: July 2024

AbstractObtaining comprehensive structural descriptions of macromolecules within their natural cellular context holds immense potential for understanding fundamental biology and improving health. Here, we present the landscape of protein synthesis inside human cells in unprecedented detail obtained using an approach which combines automated cryo-focused ion beam (FIB) milling andin situsingle-particle cryo-electron microscopy (cryo-EM). With thisin situcryo-EM approach we resolved a 2.19 Å consensus structure of the human 80S ribosome and unveiled its 21 distinct functional states, nearly all higher than 3 Å resolution. In contrast toin vitrostudies, we identified protein factors, including SERBP1, EDF1 and NAC/3, not enriched on purified ribosomes. Most strikingly, we observed that SERBP1 binds to the ribosome in almost all translating and non-translating states to bridge the 60S and 40S ribosomal subunits. These newly observed binding sites suggest that SERBP1 may serve an important regulatory role in translation. We also uncovered a detailed interface between adjacent translating ribosomes which can form the helical polysome structure. Finally, we resolved high-resolution structures from cells treated with homoharringtonine and cycloheximide, and identified numerous polyamines bound to the ribosome, including a spermidine that interacts with cycloheximide bound at the E site of the ribosome, underscoring the importance of high-resolutionin situstudiesinthe complex native environment. Collectively, our work represents a significant advancement in detailed structural studies within cellular contexts.
VIDAS®TB-IGRA accuracy in tuberculosis patients and persons at varying risk of exposure
Authors: Goletti Delia , Banaei Niaz , Batra Rahul , Berger Anne Emmanuelle , Blazevic Azra , Botelho-Nevers Elisabeth , Breen Ronan , Bruiners Natalie , Cambau Emmanuelle , Carbonnelle Etienne , Daley Charles L. , Descotes-Genon Cécile , Gennaro Francesco Di , Doucet-Populaire Florence , Esmail Aliasgar , Estrada Guzman Julia Dolores , Fontana Luc , Gennaro Maria Laura , Handler Deborah , Herrera Torres Rosa María , Hoft Daniel , Ismail Nahed , Isnard Margaux , Lardizabal Alfred , Lesage François Xavier , Lopes Amanda , López Vidal Williams Luciano , Contreras Rene Machado , Manivet Philippe , Marotte Hubert , Méchaï Frédéric , Medjahed-Artebasse Amel , Meldau Richard , Mérieux Yves , Morel Jacques , Mougari Faiza , Oelofse Suzette , Palmieri Fabrizio , Perrot Jean Luc , Petruccioli Elisa , Pride David T. , Tuaillon Edouard , Upton Caryn , Vanker Naadira , Dheda Keertan 
Published: July 2024

ABSTRACTBackgroundDetection and treatment of individuals with presumed latent tuberculosis (TB) infection (i.e., excluding active disease; LTBI) is imperative to achieve global TB control, as they represent a potential transmission reservoir. However, more sensitive and user-friendly diagnostic tools are needed.MethodsWe evaluated the accuracy for TB infection detection of the new VIDAS®TB-IGRA (bioMérieux), a fully automated, single tube (thus eliminating the need for batch testing) overnight incubation assay, compared to the QuantiFERON®-TB Gold Plus (QFT-Plus, QIAGEN), in a global multi-centre cross-sectional study (NCT04048018) that included patients with TB disease (n=200) or participants at varying levels of TB exposure (n=1460; mixed exposure-risk-population).ResultsVIDAS®TB-IGRA identified TB disease with greater sensitivity than QFT-Plus (97.5%vs. 80.7%,P<0.01%), and yielding significantly fewer false-negatives (2.5%vs. 17.5%;P<0.01%) and indeterminate results (1.0%vs. 9.5%;P=0.02%). In the mixed exposure-risk-population, negative (NPA) and positive percent agreement (PPA) were 90.1% (1097/1217) and 92.1% (223/242), respectively. PPA increased with TB-exposure risk (up to 95.7% for high-risk participants), whereas NPA decreased (starting from 96.9% for low-risk participants). Regression analyses revealed that VIDAS®TB-IGRA had a better fit with the risk-exposure gradient than the QFT-Plus. Specificity in extremely low TB-exposure risk participants (n= 125) was high for both VIDAS®TB-IGRA and QFT-Plus (97.6%vs. 95.2%;P=8.33%).ConclusionsVIDAS®TB-IGRA displayed greater sensitivity than QFT-Plus, had a lower indeterminate rate, correlated better with an exposure gradient, and was highly specific, suggesting that it is a potentially valuable tool for the diagnosis of LTBI.Take-home messageThe first fully automated interferon-γ-release assay—the bioMérieux VIDAS®TB-IGRA—is highly specific and displays greater sensitivity than QuantiFERON®-TB Gold Plus, and thus represents a valuable new and streamlined diagnostic tool for TB infection.
Tracing the transmission of carbapenem-resistantEnterobacteralesat the patient:ward environmental nexus
Authors: Elton Linzy , Williams Alan , Ali Shanom , Heaphy Jelena , Pang Vicky , Commins Liam , O’Brien Conor , Yetiş Özge , Caine Estelle , Ward Imogen , Muzslay Monika , Yui Samuel , Karia Kush , Shore Ellinor , Rofael Sylvia , Mack Damien , McHugh Timothy D , Wey Emmanuel Q 
Published: July 2024

Structured summaryIntroductionColonisation and infection with Carbapenem-resistant Enterobacterales (CRE) in healthcare settings poses significant risks, especially for vulnerable patients. Genomic analysis can be used to trace transmission routes, supporting antimicrobial stewardship and informing infection control strategies. Here we used genomic analysis to track the movement and transmission of CREs within clinical and environmental samples.Methods25 isolates were cultured from clinical patient samples or swabs, that tested positive for OXA-48-like variants using the NG-Test® CARBA-5 test and whole genome sequenced (WGS) using Oxford Nanopore Technologies (ONT). 158 swabs and 52 wastewater samples were collected from the ward environment. 60 isolates (matching clinical isolate genera;Klebsiella, Enterobacter, Citrobacter and Escherichia) were isolated from the environmental samples. Metagenomic sequencing was undertaken on 36 environmental wastewater and swab samples.Results21/25 (84%) clinical isolates had >1blaOXAgene and 19/25 (76%) harboured >1blaNDMgene. Enterobacterales were most commonly isolated from environmental wastewater samples 27/52 (51.9%), then stick swabs 5/43 (11.6%) and sponge swabs 5/115 (4.3%). 11/60 (18%) environmental isolates harboured at >1blaOXAgene and 1.9% (1/60) harbouredblaNDM-1.blaOXAgenes were found in 2/36 (5.5%) metagenomic environmental samples.DiscussionPotential for putative patient-patient and patient-ward transmission was shown. ONT sequencing can expedite clinical decisions whilst awaiting reference laboratory results, providing economic and patient care benefits. Metagenomic sampling needs optimization to improve sensitivity.
The Optimal Prediction Model for Successful External Cephalic Version
Authors: Yerrabelli Rahul Sai , Palsgaard Peggy K. , Shankarappa Priya , Jennings Valerie 
Published: July 2024

AbstractObjectiveThe majority of breech fetuses are delivered by Cesarean birth as few physicians are trained in vaginal breech birth. An external cephalic version (ECV) can prevent Cesarean delivery and the associated morbidity in these patients. Current guidelines recommend all patients with breech presentation be offered an ECV attempt. Not all attempts are successful, and an attempt does carry some risks so shared decision-making is necessary. To aid in patient counseling, over a dozen prediction models to predict ECV success have been proposed in the last few years. However, very few models have been externally validated, and thus none have been adopted into clinical practice. This study aims to use data from a United States hospital to provide further data on ECV prediction models.Study DesignThis study retrospectively gathered data from Carle Foundation Hospital and used it to test six models previously proposed to predict ECV success. These models were Dahl 2021, Bilgory 2023, López Pérez 2020, Kok 2011, Burgos 2010, and Tasnim 2012 (GNK-PIMS score).Results125 patients undergoing 132 ECV attempts were included. 69 attempts were successful (52.2%). Dahl 2021 had the greatest predictive value (AUC 0.779), while Tasnim 2012 performed the worst (AUC 0.626). The remaining models had similar predictive values as each other (AUC 0.68-0.71). Bootstrapping confirmed that all models except Tasnim 2012 had confidence intervals not including 0.5. The bootstrapped 95% AUC confidence interval for Dahl 2021 was 0.71-0.84. In terms of calibration, Dahl 2021 was well calibrated with predicted probabilities matching observed probabilities. Bilgory 2023 and López Pérez were poorly calibrated.ConclusionMultiple prediction tools have now been externally validated for ECV success. Dahl 2021 is the most promising prediction tool.Key PointsPrediction models can be powerful tools for patient counselingThe odds of ECV success can estimated based on patient factors and clinical findingsOf the 6 tested models, only Dahl 2021 appears to have good predictive value and calibration
Analysis of variants in untranslated and promoter regions and breast cancer risk using whole genome sequencing data
Authors: Wilcox Naomi , Tyrer Jonathan P. , Dorling Leila , Dennis Joe , Naven Marc , Abubakar Mustapha , Ahearn Thomas U. , Andrulis Irene L. , Antoniou Antonis C. , Bogdanova Natalia V. , Bojesen Stig E. , Bolla Manjeet K. , Brauch Hiltrud , Camp Nicola J. , Chang-Claude Jenny , Czene Kamila , Dörk Thilo , Gareth Evans D. , Fasching Peter A. , Figueroa Jonine D. , Flyger Henrik , Gardner Eugene J. , González-Neira Anna , Guénel Pascal , Hahnen Eric , Hall Per , Hartman Mikael , Hooning Maartje J. , Jakubowska Anna , Khusnutdinova Elza K. , Kristensen Vessela N. , Li Jingmei , Lindblom Annika , Lophatananon Artitaya , Mannermaa Arto , Manoukian Siranoush , Milne Roger L. , Nuñez-Torres Rocio , Obi Nadia , Panayiotidis Mihalis I. , Park Sue K. , Perry John R.B. , Rashid Muhammad U. , Saloustros Emmanouil , Sawyer Elinor J. , Schmidt Marjanka K. , Southey Melissa C. , Spurdle Amanda B. , Torres Diana , Wang Qin , Simard Jacques , Teo Soo Hwang , Dunning Alison M. , Devilee Peter , Easton Douglas F. 
Published: July 2024

AbstractRecent exome-wide association studies have explored the role of coding variants in breast cancer risk, highlighting the role of rare variants in multiple genes includingBRCA1, BRCA2, CHEK2, ATMandPALB2, as well as new susceptibility genes e.g.,MAP3K1. These genes, however, explain a small proportion of the missing heritability of the disease. Much of the missing heritability likely lies in the non-coding genome. We evaluated the role of rare variants in the 5’ and 3’ untranslated regions (UTRs) of 18,676 genes, and 35,201 putative promoter regions, using whole-genome sequencing data from UK Biobank on 8,001 women with breast cancer and 92,534 women without breast cancer. Burden tests and SKAT-O tests were performed in UTR and promoter regions. For UTR regions of 35 putative breast cancer susceptibility genes, we additionally performed a meta-analysis with a large breast cancer case-control dataset. Associations for 8 regions at P<0.0001 were identified, including several with known roles in tumorigenesis. The strongest evidence of association was for variants in the 5’UTR ofCDK5R1(P=8.5×10−7). These results highlight the potential role of non-coding regulatory regions in breast cancer susceptibility.
Neonatal microbiota colonization drives maturation of primary and secondary goblet cell mediated protection in the pre-weaning colon
Authors: Johansson Åsa , Subramani Mahadevan Venkita , Yilmaz Bahtiyar , Nyström Elisabeth , Layunta Elena , Arike Liisa , Sommer Felix , Rosenstiel Philip , Vereecke Lars , Holm Louise Mannerås , Wullaert Andy , Pelaseyed Thaher , Johansson Malin E.V. , Birchenough George M.H. 
Published: July 2024

AbstractIn the distal colon, mucus secreting goblet cells primarily confer protection from luminal microorganisms via generation of a sterile inner mucus layer barrier structure. Bacteria-sensing sentinel goblet cells provide a secondary defensive mechanism that orchestrates mucus secretion in response to microbes that breach the mucus barrier. Previous reports have identified mucus barrier deficiencies in adult germ-free mice, thus implicating a fundamental role for the microbiota in programming mucus barrier generation. In this study, we have investigated the natural neonatal development of the mucus barrier and sentinel goblet cell-dependent secretory responses upon postnatal colonization. Combinedin vivoandex vivoanalyses of pre- and post-weaning colonic mucus barrier and sentinel goblet cell maturation demonstrated a sequential microbiota-dependent development of these primary and secondary goblet cell-intrinsic protective functions, with dynamic changes in mucus processing dependent on innate immune signallingviaMyD88, and development of functional sentinel goblet cells dependent on the NADPH/Dual oxidase family member Duox2. Our findings therefore identify new mechanisms of microbiota-goblet cell regulatory interaction and highlight the critical importance of the pre-weaning period for the normal development of colonic barrier function.
Deubiquitinase JOSD1 tempers hepatic proteotoxicity
Authors: Chowdhury Saheli , Sen Abhishek , Das Debajyoti , Chakrabarti Partha 
Published: July 2024

ABSTRACTDerangements in protein homeostasis and associated proteotoxicity mark acute, chronic, and drug-induced hepatocellular injury. Metabolic dysfunction-associated proteasomal inhibition and the use of proteasome inhibitors often underlie such pathological hepatic proteotoxicity. In this study, we sought to identify a candidate deubiquitinating enzyme (DUB) responsible for reversing the proteotoxic damage. To this end, we performed a siRNA screening wherein 96 DUBs were individually knocked down in HepG2 cells under proteasomal inhibitor-induced stress for dual readouts, apoptosis, and cell viability. Among the putative hits, we chose JOSD1, a member of the Machado-Josephin family of DUBs that reciprocally increased cell viability and decreased cell death under proteotoxicity. JOSD1-mediated mitigation of proteotoxicity was further validated in primary mouse hepatocytes by gain and loss of function studies. Marked plasma membrane accumulation of monoubiquitinated JOSD1 in proteotoxic conditions is a prerequisite for its protective role while the enzymatically inactive JOSD1 C36A mutant was conversely polyubiquitinated, does not have membrane localization and fails to reverse proteotoxicity. Mechanistically, JOSD1 physically interacts with the suppressor of cytokine signaling 1 (SOCS1), deubiquitinates it and enhanced its stability under proteotoxic stress. Indeed, SOCS1 expression is necessary and sufficient for the hepatoprotective function of JOSD1.In vivo, adenovirus-mediated ectopic expression or depletion of JOSD1 in mice liver respectively protect or aggravate hepatic injury when challenged with proteasome blocker Bortezomib. Our study thus unveils JOSD1 as a potential candidate for ameliorating hepatocellular damage in liver diseases.
Temporal continuity of habitat strengthens covariation between plant and arbuscular mycorrhizal fungal communities
Authors: Shimono Ayako , Inoue Taiki , Shiga Hiroki , Uchiyama Kentaro , Yaida Yuki A. , Ushimaru Atushi , Kenta Tanaka 
Published: July 2024

AbstractOld grassland is considered one of the most biodiverse terrestrial ecosystems. The habitat temporal continuity may promote the accumulation of host species and symbiont arbuscular mycorrhizal fungi (AMF) diversity and strengthen symbiont interactions. We tested the hypothesis that covariation between AMF and plant communities is stronger in old grasslands. We compared the relationship between AMF and plant communities in forests and in new and old semi-natural grasslands in Japan. DNA was extracted from bulk roots collected at each site and from roots ofMiscanthus sinensisat each grassland site. AMF operational taxonomic unit was characterized on the basis of small subunitrRNAgene sequences. Old grasslands harboured the highest diversity of AMF among vegetation types. The AMF compositions were significantly related to those of plant species. The covariation of plant–AMF communities was stronger in old grasslands than in new grasslands. Individuals ofM. sinensiswere found to share AMF with bulk roots comprising different species. This suggests that AMF form a common mycorrhizal network among several coexisting plants of different species. Increasing AMF diversity in the shared rhizosphere has been proposed to lead to more efficient utilization of soil nutrients and to increase overall benefits of plant–AMF facilitation.
The bodily appearance of a virtual partner affects the activity of the action observation and action monitoring systems in a minimally interactive task
Authors: Pesci U.G. , Moreau Q. , Era V. , Candidi M. 
Published: July 2024

SummaryOne pending question in social neuroscience is whether interpersonal interactions are processed differently by the brain depending on the bodily characteristics of the interactor, i.e., their physical appearance. To address this issue, we engaged participants in a minimally interactive task with an avatar either showing bodily features or not while recording their brain activity using Electroencephalography (EEG) in order to investigate indices of action observation and action monitoring processing. Multivariate results showed that bodily compared to non-bodily appearance modulated parieto-occipital neural patterns throughout the entire duration of the observed movement and that, importantly, such patterns differ from the ones related to initial shape processing. Furthermore, among the electrocortical indices of action monitoring, only the early observational Positivity (oPe) was responsive to the bodily appearance of the observed agent under the specific task requirement to predict the partner movement. Taken together, these findings broaden the understanding of how bodily appearance shapes the spatiotemporal processing of an interactor’s movements. This holds particular relevance in our modern society, where human-virtual agent interactions are rapidly becoming ubiquitous.