bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transposable elements as drivers of dedifferentiation: Connections between enhancers in embryonic stem cells, placenta, and cancer
Authors: Karttunen Konsta , Patel Divyesh , Sahu Biswajyoti 
Published: July 2024

AbstractTransposable elements (TEs) have emerged as important factors in establishing the cell type‐specific gene regulatory networks and evolutionary novelty of embryonic and placental development. Recently, studies on the role of TEs and their dysregulation in cancers have shed light on the transcriptional, transpositional, and regulatory activity of TEs, revealing that the activation of developmental transcriptional programs by TEs may have a role in the dedifferentiation of cancer cells to the progenitor‐like cell states. This essay reviews the recent evidence of the cis‐regulatory TEs (henceforth crTE) in normal development and malignancy as well as the key transcription factors and regulatory pathways that are implicated in both cell states, and presents existing gaps remaining to be studied, limitations of current technologies, and therapeutic possibilities.
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass
Authors: Chitnis Atith V. , Dhoble Abhishek S. 
Published: July 2024

AbstractA reduction in the cost of production and energy requirement is necessary for developing sustainable commercial bioprocesses. Bypassing sterilization, which is an energy and cost‐intensive part of bioprocesses could be a way to achieve this. In this study, nonsterile cultivation of Yarrowia lipolytica was done on a synthetic medium containing acetic acid as the sole carbon source using two different strategies in the fed‐batch mode. The contamination percentages throughout the process were measured using flow cytometry and complemented using brightfield microscopy. Maximum biomass and lipid yields of 0.57 (g biomass/g substrate) and 0.17 (g lipids/g substrate), respectively, and maximum biomass and lipid productivities of 0.085 and 0.023 g/L/h, respectively, were obtained in different fed‐batch strategies. Feeding at the point of stationary phase resulted in better biomass yield and productivity with less than 2% contamination till 48 h. Feeding to maintain a minimum acetic level resulted in better lipid yield and productivity with less than 2% contamination during the complete process. The results of this study demonstrate the potential for cultivating Y. lipolytica in nonsterile conditions and monitoring the contamination throughout the process using flow cytometry.
Prenylated Acetophenone Derivatives from the Leaves of Acronychia pedunculata and Their Anti‐Proliferative Activities
Authors: Yang Zhen‐Zhen , Li Ni‐Ping , Lv Li‐Xia , Li Yi‐Yi , Ge Ge , Zhuo Yue , Wang Qi , Gu Ji‐Hong , Wu Yan 
Published: July 2024

AbstractFour new prenylated acetophenone derivatives, including one acetophenone dimer [acronyrone D (1)] and three acetophenone monomers [acronyrones E−G (2–4)], along with seven known analogues (5–11) were obtained from the leaves of Acronychia pedunculata. Their structures and absolute configurations were established by analysis of HRMS and NMR data, single crystal X‐ray diffraction studies and quantum chemical calculations. In addition, the isolates were tested for their anti‐proliferative acivity against HCT‐116, RKO and SW480 cancer cell lines. Compound 5 exhibited significant anti‐proliferative effects on the three cell lines, with IC50 values ranging from 0.24–5.3 μM.
Insights on the Tunisian Prickly Pear Molasses as a Potential Antifibrotic and Antioxidant Candidate against Lung Fibrosis
Authors: Bahri Sana , Bouazizi Souhir , Nahdi Afef , Mlika Mona , Hamdi Mokhtar , Jameleddine Saloua 
Published: July 2024

AbstractIdiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial disease leading to pulmonary damage and respiratory failure. We aimed to investigate the effect of prickly pear molasses (PPM) on an experimental model of pulmonary fibrosis induced by bleomycin (BLM) in Wistar rat. Animals were divided into 5 groups: the control group (G1), the BLM group (G2) and three groups (G3, G4, G5) receiving a single intra‐tracheal injection of BLM (4 mg/kg) and PPM (at 2, 4.5 and10 %) that was introduced into the diet one week before BLM injection and continued for 3 weeks. Our phytochemical results revealed significant polyphenol and flavonoid content. LCMS analysis revealed the presence of Sinapinic acid, t‐ferulic acid, t‐cinnamic acid, Caffeic acid, gallic acid and vallinic acid among others. Our histological study revealed significant decrease in collagen deposition in the groups of rats treated with 4.5 % and 10 % molasses compared to BLM group. Oxidative stress in pulmonary tissues was investigated using catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) assays. Treatment with PPM normalized the disturbance in the level of these oxidative markers in G3,G4, G5 compared to G2. In conclusion, PPM exhibit antifibrotic and antioxidant activities in BLM model of lung fibrosis.
Development of Uniform Ribosome Display Technology Enabling Easy and Efficient Identification of Full‐Length Proteins that Interact with Bioactive Small and Large Molecules
Authors: Taguchi Kenshiro , Sakai Yuichi , Furuhashi Takuto , Hara Shuta , Wada Akira 
Published: July 2024

AbstractIdentifying target proteins that interact with bioactive molecules is indispensable for understanding their mechanisms of action. In this study, we developed a uniform ribosome display technology using equal‐length DNAs and mRNAs to improve molecular display principle for target identification. The equal‐length DNAs were designed to contain various coding sequences for full‐length proteins with molecular weights of up to 130 kDa and were used to synthesize equal‐length mRNAs, which allowed the formation of full‐length protein‐ribosome‐equal‐length mRNA complexes. Uniform ribosome display selections of dihydrofolate reductase and haloalkane dehalogenase mutant were performed against methotrexate and chlorohexane, respectively. Quantitative changes of proteins after each selection indicated that the target protein‐displaying ribosomal complexes were specifically selected through non‐covalent or covalent interactions with the corresponding bioactive molecules. Furthermore, selection of full‐length proteins interacting with methotrexate or anti‐DDX46 antibody from protein pools showed that only the target proteins could be precisely identified even though the molar amounts of equal‐length mRNAs encoding them were adjusted to 1/20,000 of the total equal‐length mRNAs. Thus, the uniform ribosome display technology enabled efficient identification of target proteins that interact with bioactive small and large molecules through simplified operations without deep sequencing.
HAT‐Mediated Electrochemical C(sp2)−H Alkoxylation of Pyrido[1,2‐a]pyrimidin‐4‐ones with Aliphatic Alcohols
Authors: Ghosh Subhadeep , Biswas Sumit , Das Indrajit 
Published: July 2024

AbstractA direct alkoxylation of the electron‐deficient olefinic C(sp2)−H bond in pyrido[1,2‐a]pyrimidin‐4‐ones using aliphatic alcohols has been developed under transition metal and external chemical oxidant‐free electrochemical conditions. Azidotrimethylsilane (TMSN3) is utilized as a hydrogen atom transfer (HAT) reagent to enable the homolytic cleavage of the unfunctionalized O−H bond in alcohols, thereby generating the electrophilic alkoxy radicals. Moreover, the effectiveness of this method is demonstrated with d4‐methanol (CD3OD), leading to the synthesis of d3‐methoxylated N‐heterocycles in good yields under sustainable conditions. The proposed mechanism, based on alkoxy or trideuteromethoxy radicals, is substantiated by control experiments and cyclic voltammetry (CV) studies.
Advancements in Photocatalytic H2O2 Production via Carbon Vacancy‐Engineered g‐C3N4
Authors: Wang Lin , Hou Huilin 
Published: July 2024

AbstractHydrogen peroxide (H2O2) is gaining prominence as a versatile oxidizing agent and sustainable energy carrier, prompting the need for efficient and eco‐friendly production methods. Traditional approaches face challenges such as high energy consumption and environmental impact, driving interest towards alternative methods like photocatalysis and electrocatalysis. Graphitic carbon nitride (g‐C3N4) has emerged as a promising photocatalyst due to its inherent band structure that facilitates solar energy utilization. However, bare g‐C3N4 encounters limitations like rapid charge recombination and limited light absorption. Recent advancements have focused on enhancing g‐C3N4 photocatalysts through carbon vacancy (Cv) engineering, which effectively modifies electronic and surface properties to improve photocatalytic performance. This mini‐review explores the fundamental principles and recent advancements in Cv‐engineered g‐C3N4 photocatalysts for H2O2 production. It discusses the critical roles of carbon vacancies in enhancing the photocatalytic performance of g‐C3N4, along with methods for creating carbon vacancies in g‐C3N4 and their impact on photocatalytic H2O2 production. The review also addresses challenges related to scalable synthesis and practical deployment of Cv‐g‐C3N4 materials, providing insights into future research directions for sustainable photocatalytic technologies.
Electrolytes for Aluminum‐Ion Batteries: Progress and Outlook
Authors: Liu Wanxin , Li Le , Yue Shi , Jia Shaofeng , Wang Conghui , Zhang Dan 
Published: July 2024

AbstractAluminum‐ion batteries (AIBs) are promising electrochemical energy storage sources because of their high theoretical specific capacity, light weight, zero pollution, safety, inexpensiveness, and abundant resources. These theoretical advantages have recently made AIBs a research hotspot. However, electrolyte‐related issues significantly limit their commercialization. The electrolyte choices for AIBs are significantly limited, and most of the available options do not facilitate the Al3+/Al three‐electron transfer reaction. Thus, this review presents an overview of recent advances in electrolytes and modification strategies for AIBs to clarify the limitations of existing AIB electrolytes and offer guidance for improving their performance. Furthermore, herein, the advantages, limitations and possible solutions for each electrolyte are discussed, after which the future of AIB electrolytes is envisioned.
Symmetric Aromatic Amidoalkylations of Triphenylenes
Authors: Lorenzetto Tommaso , Berton Giacomo , Castellini Francesco , Casson Gabriele , Scarso Alessandro , Fabris Fabrizio 
Published: July 2024

AbstractTriphenylene derivatives are highly investigated for their electronic, supramolecular and photophysical properties, but the direct modification of the central aromatic core is particularly challenging especially in the internal positions 1, 4, 5, 8, 9, and 12. Herein we present an efficient alkylation method of 2,3,6,7,10,11‐hexasubstituted triphenylene derivatives leading to tris‐alkylated C3‐symmetric derivatives in good yields using N‐(hydroxymethyl)carboxamide or N‐(alkoxylmethyl)carboxamide alkylating agents.
Modulation of the multiphase phosphorus/sulfide heterogeneous interface via rare earth for solar‐enhanced water splitting at industrial‐level current densities
Authors: Cheng Yikun , Fu Pengjie , Yu Zhipeng , Yang Xiaodong , Zhang Yangrui , Yuan Aojie , Liu Huan , Du Jianhao , Chen Long 
Published: July 2024

AbstractPhotoelectrically coupling water splitting at high current density is a promising approach for the acquisition of green hydrogen energy. However, it places significant demands on the photo/electrocatalysts. Herein, rare earth elements doping NiMoO4‐based phosphorus/sulfide heterostructure nanorod arrays (RE‐NiMo‐PS@NF [RE = Y, Er, La, and Sc]) are obtained for solar‐enhanced electrocatalytic water splitting at high current densities. The results of the experiment and density‐functional theory studies illustrate that the Y element as a dopant not only makes the NiMoP2/NiMo3S4/NiMoO4 heterostructure exhibit excellent solar‐enhanced electrocatalytic activity (hydrogen evolution reaction [HER]: η1000 = 211 mV, oxygen evolution reaction [OER]: η1000 = 367 mV) but also optimizes the heterostructure interfacial electron density distributions and HER free energy. In addition, Y‐NiMo‐PS@NF achieves 18.64% solar‐to‐hydrogen efficiency. This study not only provides a new way to synthesize heterostructure electrocatalysts but also inspires the application of solar enhancement strategies for high current density water splitting.
Integrated Cyano Groups into the Skeleton of Conjugated Polymers to Activate Molecular Oxygen for Boosting Photocatalytic H2O2 Efficiency
Authors: Wang Qing , Wang Wenjiao , Guo Liping , Liao Lijun , Li Zhenzi , Xiang Yonggang , Wang Xuepeng , Liu Haixia , Zhou Wei 
Published: July 2024

AbstractConjugated polymers (CPs) have shown promising potential in the field of hydrogen peroxide (H2O2) photosynthesis. However, a deeper understanding of the interactions between building units and specific functional groups within the molecular skeleton is necessary to elucidate the mechanisms driving H2O2 generation. Herein, a series of typical donor‐acceptor (D‐A) conjugated polymers (B‐B, B‐CN, B‐DCN) were synthesized by introducing different amounts of cyano groups (‐CN) into the molecular skeleton. The strong electron withdrawing properties of cyano can greatly promote the effective separation and transfer of photogenerated charges between building units, resulting in an impressive efficiency of H2O2 generation (2128.5 μmol g−1 h−1) for B‐DCN, representing a 96‐fold enhancement compared to B‐B. More importantly, experimental results and theoretical calculations further revealed that the introduction of ‐CN can markedly reduce the adsorption energy (Ead) of O2, while serving as an active site to induce the conversion of crucial intermediate superoxide anions (⋅O2−) into singlet oxygen (1O2), achieving dual‐channel H2O2 generation (O2→⋅O2−→H2O2, O2→⋅O2−→1O2→H2O2). This work provides valuable insights into the design of efficient H2O2 photosynthesis materials.
Analysis of lens cloudiness during endoscopic submucosal dissection procedures: Effects of a novel lens cleaner
Authors: Fujii Takashi , Watanabe Sho , Uga Misugi , Matsui Yuuki , Sakaki Kazuomi , Matsukawa Naoki , Machida Tomoyo , Kurihara Masamichi , Tashiro Yoshihiro , Okamoto Eiko , Yauchi Tsunehito , Suzuki Shinji , Koyama Shigeru 
Published: July 2024

AbstractObjectivesWe aimed to identify independent factors for intraoperative endoscopic lens cloudiness during gastric and colorectal endoscopic submucosal dissections, investigate the effectiveness of Cleastay, an endoscope anti‐fog solution, and examine factors associated with severe submucosal fat deposition.MethodsA total of 220 patients who underwent gastric or colorectal endoscopic submucosal dissections in two institutions between January 2022 and October 2023 were included. Significant factors related to cloudiness were determined using univariate and multivariate analyses. Patient background and tumor characteristics related to severe submucosal fat deposition were investigated, and the degree of intraoperative endoscopic lens cloudiness and outcomes were compared between the Cleash and Cleastay groups.ResultsIn the multivariate analysis, factors increasing lens cloudiness included long procedure time (odds ratio [OR], 17.51; 95% confidence interval [CI], 1.52–202.08), stomach (vs. colon; OR, 5.08; 95% CI, 1.99–12.96), and severe submucosal fat deposition (OR, 12.19; 95% CI, 5.02–29.60). Conversely, the use of Cleastay (vs. Cleash; OR, 0.066; 95% CI, 0.021–0.21) was identified as a factor reducing cloudiness. Location analysis revealed that severe submucosal fat deposition was more common in the upper stomach and right colon.ConclusionsIt was suggested that Cleastay is more useful for endoscopic submucosal dissection of the upper stomach and right colon, where severe submucosal fat deposition is expected.
Coronary microvascular dysfunction and left heart filling pressures in patients with type 2 diabetes
Authors: Wolsk Emil , Jürgens Mikkel , Schou Morten , Ersbøll Mads , Hasbak Philip , Kjaer Andreas , Zerahn Bo , Høgh Brandt Niels , Haulund Gæde Peter , Rossing Peter , Faber Jens , Kistorp Caroline Michaela , Gustafsson Finn 
Published: July 2024

AbstractAimsPatients with type 2 diabetes (T2D) have a high prevalence of diastolic dysfunction and heart failure with preserved ejection fraction (HFpEF), which in turn leads to an increased risk of hospitalization and death. However, the factors of risk and their relative importance in leading to higher left ventricular filling pressures are still disputed. We sought to clarify the associations of a wide range of invasive and non‐invasive risk factors with cardiac filling pressures in high‐risk T2D patients.Methods and resultsPatients with T2D at high risk of cardiovascular events were prospectively enrolled in this study. Participants were thoroughly phenotyped including right heart catheterization at rest and during exercise, echocardiography, urinary excretion of albumin (UACR), and quantification of their myocardial blood flow rate (MFR) using cardiac 82Rb‐PET/CT. Of the 37 patients included in the study, 22 (59%) patients met invasive criteria for HFpEF. Only 2 out of 39 variables emerged as independent factors associated with left ventricular filling pressure as assessed by pulmonary capillary wedge pressure (PCWP) at rest; history of hypertension (coefficient: 2.6 mmHg [0.3; 5.0], P = 0.030) and MFR (P = 0.026). We found a significant inverse association between MFR and PCWP with a coefficient of −2.3 mmHg (−4.3; −0.3) in PCWP per integer change of MFR. The MFR ranged from 1.18 to 3.68 in our study, which corresponds to a difference in PCWP of approximately 6 mmHg between patients with the lowest compared to the highest MFR. During exercise, only 2 variables emerged as borderline independent factors associated with PCWP: myocardial flow reserve (coefficient: −4.4 [−9.6; 0.8], P = 0.091) and beta‐blockers use (coefficient: 6.1 [−0.1; 12.4], P = 0.053).ConclusionsIn patients with type 2 diabetes without known HFpEF but risk factors for cardiovascular disease, myocardial blood flow rate was independently associated with PCWP at rest across the range from normal to abnormal left heart filling pressures. A clinically significant difference of 6 mmHg in PWCP was attributable to differences in MFR in patients with the lowest compared with the highest MFR values. This suggests that strategies than attenuate microvascular dysfunction could slow progression of increased left ventricular left heart filling pressures in patients at increased risk.
Structures and NMR Solution Dynamics of Diorganotin Complexes with Bulky Quadridentate Salen‐ and Salphen‐Type Schiff Bases (N2O2) Incorporating Diazenylpyridyl Side Arms
Authors: Basu Baul Tushar S. , Brahma Swmkwr , Rao Addepalli Maheswara , Duthie Andrew , Mahmoud Abdallah G. , Guedes da Silva M. Fátima C. , Parkin Sean 
Published: July 2024

AbstractA series of six complexes [n‐Bu2Sn(L1)]⋅S (S=Benzene/toluene, mixed/partial) (1), [Ph2Sn(L1)]n⋅S (S=Benzene) (2), [Bz2Sn(L1)] (3), [n‐Bu2Sn(L2)]n (4), [Ph2Sn(L2)] (5) and [Bz2Sn(L2)] (6) were synthesized using diorganotin(IV) oxide precursors and two new bis‐Schiff bases containing N2O2 donor atoms. The Schiff bases, H2L1 and H2L2, were prepared by reacting ethane‐1,2‐diamine or benzene‐1,2‐diamine with 2‐hydroxy‐5‐(pyridin‐3‐yldiazenyl)benzaldehyde in absolute ethanol. Compounds 1–6 were characterized by IR and NMR spectroscopies, as well as by solid‐state single‐crystal X‐ray diffraction (except for 3 and 6). In all the complexes the bis‐Schiff bases occupy the equatorial positions and the R groups are in axial sites. However, while 1 and 5 are discrete molecules where the tin atoms adopt distorted octahedral geometries, 2 and 4 are monoperiodic polymers with the metal cations in pentagonal bipyramidal environments. Although solid state crystallographic results revealed asymmetrical molecular configurations, solution NMR studies proved symmetric arrangements for all the compounds. Tin NMR spectroscopy confirmed that all complexes are six‐coordinate in solution, despite variations in solid‐state coordination. Further analysis using Hirshfeld surface and fingerprint plots provided insights into intermolecular interactions in compounds 1, 2, 4, and 5. This comprehensive study underscores the structural diversity and solution‐state dynamics of organotin(IV) complexes synthesized using novel Schiff bases.
Rapid and reliable determination of sulfide and sulfite in food by a multianalyte electrochemical sensor based on nanoporous gold
Authors: Wang Xiaolei , Qi Xiaoyan , Liu Ruijun , Cai Ting , Huang Zihan , Wang Sen , Wang Xia 
Published: July 2024

AbstractGiven the increasing food safety problems caused by sulfide and sulfite, there is an urgent need to develop a rapid and accurate method for the detection of sulfide and sulfite in food. In this study, a multianalyte electrochemical sensor was successfully fabricated based on glassy carbon electrode (GCE) modified by nanoporous gold (NPG) for the detection of sulfide and sulfite. Owing to the exceptional oxidation activity of NPG towards sulfite and sulfide, the constructed multianalyte NPG/GCE electrochemical sensor achieved highly sensitive detection of sulfide in the concentration range of 50 μM–5 mM with a sensitivity of 21.33 μA mM−1cm−2 and a limit of detection (LOD) as low as 37.53 μM. For the detection of sulfite, the multianalyte NPG/GCE electrochemical sensor exhibited good linearity in the concentration range of 50 μM–5 mM with a sensitivity of 71.76 μA mM−1cm−2 and a LOD of 5.12 μM. In addition, the multianalyte NPG/GCE electrochemical sensor realized the reliable detection of sulfide and sulfite in complex real food samples (such as milk, pickles, red wine, and tap water) with high sensitivity and anti‐interference ability. The multianalyte NPG/GCE electrochemical sensor exhibited many advantageous properties in practical applications, including easy fabrication, sensitivity, rapidity, cost‐efficient, and flexible adaptability, which made it a promising candidate for the rapid and reliable detection of sulfide and sulfite in food.
An adjustable high‐flux LED solar simulator based on dome structure
Authors: Xue Chang‐Wen , Song Jia‐Yong , Qin Ze‐Sheng , Bian Li‐Feng , Luo Zi‐Jiang , Yang Chen 
Published: July 2024

AbstractHigh‐flux solar simulator (HFSS) commonly serves as a vital instrument for conducting material testing and thermochemical experiments, offering valuable applications in the fields of photovoltaic cells and concentrated solar energy. This paper proposes a continuously adjustable HFSS based on light‐emitting diodes (LEDs), which can be employed for experimental testing in the solar cell aging. First, an irradiation unit module has been built using high‐power LEDs and total internal reflection lenses, and the irradiation performance of the single unit has been validated. In theory, a dome layout model is proposed, in which a detailed geometric analysis is provided for the maximum number of units that can be accommodated on the dome, considering unit size and dome dimensions. Subsequently, aluminum disc has been used as thermal flux sensors, and the irradiation distribution of the system is characterized using a charge‐coupled device observation camera and Lambertian board. The results indicate that the system offers an adjustable average flux ranging from 1.6 to 9.04 kW/m2 when the system input current is in the range of 7.2–54 A. Additionally, the system demonstrates a spatial nonuniformity of 2% within a 10‐mm diameter (Φ = 10 mm) region test region and temporal instability of 2% within 30 min.