bluuebunny/crossref_metadata_2025_split huggingface dataset:
Missing Data in Long‐term Follow‐up of Patients with Eating Disorders Using the Body Attitude Test
Authors: Geerdens Candida , Vanderlinden Johan , Pieters Guido , De Herdt Amber , Probst Michel 
Published: October 2012

AbstractObjectiveIncompleteness of data is a major problem within clinical follow‐up studies. The aim of present study was to compare different statistical models in the management of follow‐up data in patients with eating disorders using the Body Attitude Test.MethodA prospective longitudinal investigation with repeated evaluations over fixed time intervals was conducted among 807 female patients with eating disorders using the Body Attitude Test as a dependent‐variable. Three types of missing data mechanisms were explored: missing completely at random, missing at random and missing not at random.ResultsMultivariate analysis showed that the missing completely at random type of missing data mechanism is less reliable than the missing at random or missing not at random mechanisms. Five years after admission, the body experience of eating disorder patients is less deviant. Compared with normative data, 37% of the eating disorder patients still had a more negative body experience 5 years after admission.DiscussionThere is no single correct method for dealing with missing data. Therefore, it is recommended that multiple methods be used under different assumptions of absenteeism. Copyright © 2012 John Wiley & Sons, Ltd and Eating Disorders Association.
Unhealthy Weight‐control Behaviours, Dieting and Weight Status: A Cross‐cultural Comparison between North American and Spanish Adolescents
Authors: López‐Guimerà Gemma , Neumark‐Sztainer Dianne , Hannan Peter , Fauquet Jordi , Loth Katie , Sánchez‐Carracedo David 
Published: October 2012

AbstractThe aim of the current study was to examine and compare dieting and unhealthy weight‐control behaviours (UWCB) in population‐based samples in two large urban areas in Spain (Barcelona) and in the USA (Twin Cities of Minneapolis and St. Paul, Minnesota). Additionally, use of UWCB across weight categories was explored in both samples. Participants included 1501 adolescents from Barcelona (48% girls, 52% boys) and 2793 adolescents from the Twin Cities (53% girls, 47% boys). The main outcome measures were dieting, UWCB (less extreme and extreme) and weight status. Although dieting and UWCB were prevalent in both samples, particularly among girls, the prevalence was higher in the US sample. In both countries, the report of dieting and use of UWCB was highest among overweight and obese youth. Prevention interventions that address the broad spectrum of eating and weight‐related problems should be warranted in light of the high prevalence and co‐occurrence of overweight and unhealthy weight‐related behaviours. Copyright © 2012 John Wiley & Sons, Ltd and Eating Disorders Association.
Natural and historical variability in fluvial processes, beaver activity, and climate in the Greater Yellowstone Ecosystem
Authors: Persico Lyman , Meyer Grant 
Published: October 2012

ABSTRACTTwo centuries of human activities in the Greater Yellowstone Ecosystem (GYE) have strongly influenced beaver activity on small streams, raising questions about the suitability of the historical (Euro‐American) period for establishing stream reference conditions. We used beaver‐pond deposits as proxy records of beaver occupation to compare historical beaver activity to that throughout the Holocene. Forty‐nine carbon‐14 (14C) ages on beaver‐pond deposits from Grand Teton National Park indicate that beaver activity was episodic, where multi‐century periods lacking dated beaver‐pond deposits have similar timing to those previously documented in Yellowstone National Park. These gaps in the sequence of dated deposits coincide with episodes of severe, prolonged drought, e.g. within the Medieval Climatic Anomaly 1000–600 cal yr bp, when small streams likely became ephemeral. In contrast, many beaver‐pond deposits date to 500–100 cal yr bp, corresponding to the colder, effectively wetter Little Ice Age. Abundant historical beaver activity in the early 1900s is coincident with a climate cooler and wetter than present and more abundant willow and aspen, but also regulation of beaver trapping and the removal of wolves (the beaver's main predator), all favorable for expanded beaver populations. Reduced beaver populations after the 1920s, particularly in the northern Yellowstone winter range, are in part a response to elk overbrowsing of willow and aspen that later stemmed from wolf extirpation. Beaver populations on small streams were also impacted by low streamflows during severe droughts in the 1930s and late 1980s to present. Thus, both abundant beaver in the 1920s and reduced beaver activity at present reflect the combined influence of management practices and climate, and underscore the limitations of the early historical period for defining reference conditions. The Holocene record of beaver activity prior to Euro‐American activities provides a better indication of the natural range of variability in beaver‐influenced small stream systems of the GYE. Copyright © 2012 John Wiley & Sons, Ltd.
Hierarchical underwater acoustic sensor networks with (virtual) transmit/receive arrays
Authors: Stefanov Andrej , Stojanovic Milica 
Published: October 2012

ABSTRACTWe consider a hierarchical underwater acoustic sensor network architecture in which the sensors and the collector stations operate in distinct layers. The hierarchical architecture is motivated by the property of the acoustic underwater transmission medium that for each transmission distance, there exists an operating frequency for which the narrow‐band signal‐to‐noise ratio is maximised. The sensors and the collector stations are consequently allocated different operating frequencies. We assume a uniform distribution of both sensors and collector stations over the finite area of the sensing field. The sensors are organised into clusters forming virtual transmit/receive arrays. The collector stations, on the other hand, are equipped with co‐located transmit/receive arrays. We adopt a communication‐theoretic approach and study the interdependence of the sustainable number of hops through the network, end‐to‐end frame error probability, power and bandwidth allocation. The analysis is performed under the assumption of Ricean fading and interference from other nodes within the same layer of the hierarchy. We present numerical examples that illustrate the network performance and demonstrate that there are preferred operating frequencies, which ensure network operation without any cross‐interference between the collector network and the sensor network. Copyright © 2012 John Wiley & Sons, Ltd.
Chronic lymphocytic leukemia with del13q14 as the sole abnormality: dynamic prognostic estimate by interphase‐FISH
Authors: Orlandi Ester M. , Bernasconi Paolo , Pascutto Cristiana , Giardini Ilaria , Cavigliano Paola M. , Boni Marina , Zibellini Silvia , Cazzola Mario 
Published: October 2012

AbstractThis study analyzed 140 patients with isolated del13q14 on interphase FISH (I‐FISH), to identify subsets with a different progression risk and to assess the acquisition of additional chromosomal abnormalities (clonal evolution) in treatment‐naïve del13q14 patients. A monoallelic deletion (del13qx1) was detected in 123 cases (88%), a biallelic deletion (del13qx2) in eight and a mosaic of monoallelic and biallelic deletions (del13qx1/del13qx2) in nine. In 33% of cases, deletion encompassed the Rb1 locus The median percentage of abnormal nuclei was 50% (15%–96%), and it was higher in patients with a biallelic/mosaic pattern in comparison with patients with monoallelic deletion. Sixty two patients (44%) have been treated; 5‐year treatment free survival rate was 56% and the median treatment free survival was 65 months. The baseline percentage of deleted nuclei, as a continuous variable, was related to progression (HR: 1.02; p = 0.001). According to deletion burden, three groups were identified: 64 cases (46%) had <50% deleted nuclei, 47 (33%) had 50–69% deleted nuclei, and 29 (21%) had ≥70% deleted nuclei. The 5‐year untreated rate was 70.5% , 52.6% and 28.7% (p < 0.0001), respectively. In multivariate analysis using IGHV mutational status, presence of a nullisomic clone, CD38 expression and percentage of deleted nuclei as covariates, only IGHV mutational status and the percentage of deleted nuclei were independent risk factors for treatment. In 103 patients serially monitored by I‐FISH before starting any treatment, we observed a significant increase in the proportion of del13q14 cells, and this increase affected the risk of subsequent treatment requirement (HR 2.54, p = 0.001). The appearance of a new clone was detected in 16 patients (15.5%) and chromosome 13 was involved in 14 of them. I‐FISH monitoring proves worthwhile for a dynamic risk stratification and for planning clinical surveillance. Copyright © 2012 John Wiley & Sons, Ltd.
Dexamethasone prevents monocyte‐induced tubular epithelial–mesenchymal transition in HK‐2 cells
Authors: Li Qing , Lv Lin‐Li , Wu Min , Zhang Xiao‐Liang , Liu Hong , Liu Bi‐Cheng 
Published: October 2012

AbstractEpithelial–mesenchymal transition (EMT) is a key cellular event in the early stage of tubulointerstitial fibrosis (TIF). Monocyte infiltration plays an important role in the progression of TIF. We have previously demonstrated that monocytes can directly induce HK‐2 cell transition by direct contact. Dexamethasone, an important anti‐inflammatory and immunosuppressant agent, has been widely used in renal disease for decades. Whether it could influence the monocyte and HK‐2 cell interaction and prevent EMT is still uncertain. In this study, we found that the typical epithelial cell morphology of HK‐2 cells disappeared 24 h after co‐culture with monocytes, and dexamethasone significantly prevented this change in a dose‐dependent manner. In addition, we found that dexamethasone prevented monocytes from binding to HK‐2 cells by inhibiting ICAM‐1 expression on HK‐2 cells. Further analysis demonstrated that there was increased E‐cadherin expression and decreased α‐SMA and fibronectin expression after co‐culture with dexamethasone, suggesting that dexamethasone prevents monocyte‐induced HK‐2 cell transition. The nuclear transcription factor κB (NF‐κB) pathway played an important role in this process. These findings suggest a novel mechanism by which corticosteroids may delay the progression of TIF via preventing EMT. J. Cell. Biochem. 114: 632–638, 2013. © 2012 Wiley Periodicals, Inc.
3,4‐dimethoxystilbene, a resveratrol derivative with anti‐angiogenic effect, induces both macroautophagy and apoptosis in endothelial cells
Authors: Zhang Lu , Jing HongJuan , Cui LiuQing , Li HuanQing , Zhou Bo , Zhou GuangZhou , Dai Fang 
Published: October 2012

AbstractAngiogenesis plays an important role in many pathological processes. Identification of novel anti‐angiogenic agents will provide new insights into the mechanisms for angiogenesis as well as potential lead compounds for developing new drugs. In the present study, a series of resveratrol methylated derivatives have been synthesized and screened. We found trans‐3,4‐dimethoxystilbene (3,4‐DMS) with the fullest potential to develop as an anti‐angiogenic agent. In vitro and in vivo analyses suggested that 3,4‐DMS could effectively inhibit endothelial cell proliferation, migration, tube formation, and endogenous neovascularization. Our results showed that 3,4‐DMS exerted its anti‐angiogenic effect likely through induction of endothelial cell apoptosis via a pathway involving p53, Bax, cytochrome c, and caspase proteases. Moreover, 3,4‐DMS also induced macroautophagy in endothelial cells through activation of AMPK and the downstream inhibition of mTOR signaling pathway. Further studies indicated that intracellular calcium ([Ca2+]i) might bridge the 3,4‐DMS‐induced apoptosis and macroautophagy through modulating reactive oxygen species (ROS) levels in endothelial cells. Combination of 3,4‐DMS with inhibitor of autophagy, such as 3‐methyladenine (3‐MA) and autophagy‐related gene (ATG) 5 small interfering RNA (siRNA), potentiated the pro‐apoptotic and anti‐angiogenic effects of 3,4‐DMS. Our study provides a novel angiogenic inhibitor and a useful tool in exploring the molecular mechanisms for the crosstalk between apoptosis and macroautophagy in endothelial cells. 3,4‐DMS could be served as a potential lead compound for developing a class of new drugs targeting angiogenesis‐related diseases. J. Cell. Biochem. 114: 697–707, 2013. © 2012 Wiley Periodicals, Inc.
Activation and function of murine Cyclin T2A and Cyclin T2B during skeletal muscle differentiation
Authors: Marchesi Irene , Nieddu Valentina , Caracciolo Valentina , Maioli Margherita , Gaspa Leonardo , Giordano Antonio , Bagella Luigi 
Published: October 2012

AbstractCyclin‐dependent kinase 9 (Cdk9) is a serine‐threonine kinase, involved in many cellular processes. The regulatory units of Cdk9 are the T family Cyclins (T1, T2) and Cyclin K. Cyclin T2 has two forms termed Cyclin T2a and Cyclin T2b that arise by an alternative splicing of the primary transcript. Upon induction of muscle differentiation, MyoD recruits Cdk9/Cyclin T2 on muscle‐specific gene promoter sequences. This complex is able to phosphorylate the C‐terminal domain of RNA polymerase II, enhancing MyoD function and promoting myogenic differentiation. This work focuses on the characterization of two murine Cyclin T2 isoforms and the evaluation of the role of Cdk9/Cyclin T2 complexes during the skeletal muscle differentiation. This study demonstrated a predominant expression of isoform b in all stages of differentiation. Moreover, both isoforms of Cyclin T2 are able to activate the myogenic program but Cyclin T2b has a predominant role, in particular during the latest stages. J. Cell. Biochem. 114: 728–734, 2013. © 2012 Wiley Periodicals, Inc.
Fibronectin‐mediated cell spreading requires ABBA‐Rac1 signaling
Authors: Zeng Xian‐Chun , Luo Xuesong , Wang San‐Xia , Zhan Xi 
Published: October 2012

AbstractABBA was reported to be an actin dynamics regulator. However, the molecular mechanism of action of ABBA is still totally obscure. Here, we show that ABBA is ubiquitously expressed in all the examined cultured cells. We found that expression of ABBA in NIH3T3 cells promotes cell spreading. ABBA binds to and markedly promotes cell spreading‐induced Rac1 activation. Cell spreading stimulates ABBA activation probably by inducing it tyrosine phosphorylation, which endows ABBA much higher activity to activate Rac1, and attenuates the interaction between ABBA and Rac1. Loss of function suggests that deletion of ABBA in C6‐R cells markedly inhibits Rac1 activation and cell spreading; this suggests that and the interaction between ABBA and activated Rac1 is required for ABBA‐promoted cell spreading. Taken together, our results indicate that ABBA is activated in response to cell spreading, which markedly promotes cell spreading, and ABBA is required for Rac1 activation and cell spreading. J. Cell. Biochem. 114: 773–781, 2013. © 2012 Wiley Periodicals, Inc.
c‐Met function requires n‐linked glycosylation modification of pro‐Met
Authors: Chen Run , Li Juan , Feng Chun‐Hong , Chen Shao‐Kun , Liu You‐Ping , Duan Chun‐Yan , Li Hong , Xia Xian‐Ming , He Tao , Wei Mei , Dai Rong‐Yang 
Published: October 2012

Abstractc‐Met, the receptor for hepatocyte growth factor (HGF), is cell surface tyrosine kinase that controls cancer cell growth, survival, invasion, and metastasis. Post‐translational modification, such as glycosylation, plays an essential role in regulating the function of cell surface molecules. Whether glycosylation modification regulates the enzymatic properties of c‐Met is unknown. In this study, we investigated the effect of glycosylation on the function of c‐Met. We found that c‐Met is an N‐linked glycosylated protein. Both pro‐Met and p145Met (the β subunit of mature c‐Met) have N‐linked glycosylation. Glycosylation inhibitor studies revealed that the N‐glycosylation modification of p145Met is from pro‐Met, but not due to the further modification of pro‐Met. Importantly, blocking the N‐glycosylation targets pro‐Met to cytoplasm and initiates its phosphorylation independent of HGF engagement. Nonglycosylated pro‐Met activates c‐Met downstream pathways to a certain extent to compensate for the degradation of p145Met induced by glycosylation blocking‐mediated endoplasmic reticulum (ER) stress. J. Cell. Biochem. 114: 816–822, 2013. © 2012 Wiley Periodicals, Inc.
The Inheritance of Process: A Dynamical Systems Approach
Authors: Jaeger Johannes , Irons David , Monk Nick 
Published: October 2012

AbstractA central unresolved problem of evolutionary biology concerns the way in which evolution at the genotypic level relates to the evolution of phenotypes. This genotype–phenotype map involves developmental and physiological processes, which are complex and not well understood. These processes co‐determine the rate and direction of adaptive change by shaping the distribution of phenotypic variability on which selection can act. In this study, we argue—expanding on earlier ideas by Goodwin, Oster, and Alberch—that an explicit treatment of this map in terms of dynamical systems theory can provide an integrated understanding of evolution and development. We describe a conceptual framework, which demonstrates how development determines the probability of possible phenotypic transitions—and hence the evolvability of a biological system. We use a simple conceptual model to illustrate how the regulatory dynamics of the genotype–phenotype map can be passed on from generation to generation, and how heredity itself can be treated as a dynamic process. Our model yields explanations for punctuated evolutionary dynamics, the difference between micro‐ and macroevolution, and for the role of the environment in major phenotypic transitions. We propose a quantitative research program in evolutionary developmental systems biology—combining experimental methods with mathematical modeling—which aims at elaborating our conceptual framework by applying it to a wide range of evolving developmental systems. This requires a large and sustained effort, which we believe is justified by the significant potential benefits of an extended evolutionary theory that uses dynamic molecular genetic data to reintegrate development and evolution. J. Exp. Zool. (Mol. Dev. Evol.) 9999B:591–612, 2012. © 2012 Wiley Periodicals, Inc.
Two‐dimensional J‐resolved proton MR spectroscopy and prior knowledge fitting (ProFit) in the frontal and parietal lobes of healthy volunteers: Assessment of metabolite discrimination and general reproducibility
Authors: Prescot Andrew P. , Renshaw Perry F. 
Published: October 2012

AbstractPurpose:To investigate human brain metabolite discriminability and general measurement reproducibility of two‐dimensional (2D) J‐resolved 1H MRS and Prior Knowledge Fitting (ProFit).Materials and Methods:2D J‐resolved 1H MRS spectra were acquired from the anterior cingulate cortex (ACC) and the parietal‐occipital cortex (POC) of 10 healthy subjects at a magnetic field strength of 2.9 Tesla. Amplitude correlation matrices were constructed for each subject and brain region to assess metabolite discriminability. ProFit‐estimated metabolite peak areas were normalized to a water reference signal, and intra‐ and inter‐subject reproducibility was evaluated.Results:Favorable between‐metabolite correlation coefficients (<20%) were observed for a range of metabolites. Lower correlation coefficients between a given pair of metabolite estimates were consistently observed for POC metabolites. The group mean correlation coefficient existing between glutamate and glutamine was calculated as −18% and −13% for ACC and POC, respectively. Most ACC and POC metabolites showed intra‐ and inter‐subject CV values of <15% and <20%, respectively.Conclusion:The observed Glu and Gln signal discrimination makes these techniques suitable for investigating a variety of psychiatric disorders. Intra‐ and inter‐subject metabolite level reproducibility was comparable to the existing literature findings. These data serve as a valuable benchmark for assessing future modifications to 2D 1H MRS data acquisition and ProFit analysis. J. Magn. Reson. Imaging 2013;37:642–651. © 2012 Wiley Periodicals, Inc.
Detectability of hepatocellular carcinoma by gadoxetate disodium‐enhanced hepatic MRI: Tumor‐by‐tumor analysis in explant livers
Authors: Nakamura Yuko , Tashiro Hirotaka , Nambu Junko , Ohdan Hideki , Kakizawa Hideaki , Date Shuji , Awai Kazuo 
Published: October 2012

AbstractPurpose:To investigate the detectability of hepatocellular carcinoma (HCC) on Gd‐EOB‐enhanced MR images (Gd‐EOB‐MRI), we performed tumor‐by‐tumor analysis of pathologically confirmed tumors using explants from cirrhotic patients who had undergone liver transplantation.Materials and Methods:We studied 11 explanted livers and classified the tumor intensity during the arterial phase (AP) and the hepatobiliary phase (HBP) as low in HBP with early enhancement (EE) in AP (A), as high in HBP with EE in AP (B), as low in HBP without EE in AP (C), as high in HBP without EE in AP (D), and as iso‐intense in HBP with EE in AP (E). The diagnostic criteria for HCC were (i) pattern A and C, (ii) pattern A and E, (iii) pattern C and E, and (iv) patterns A, C, and E.Results:Of the 71 HCCs, 22 were not detected at MRI; of these, 9 were moderately differentiated and 13 were well‐differentiated HCCs. The sensitivity of Gd‐EOB‐MRI for diagnostic criteria 1, 2, 3, and 4 was 63.4%, 52.1%, 22.5%, and 69.0%.Conclusion:The maximum sensitivity of Gd‐EOB‐MRI for HCC was only 69.0% even when diagnostic criteria that included all previously reported HCC patterns were adopted. J. Magn. Reson. Imaging 2013;37:684—691. © 2012 Wiley Perioidicals, Inc.
Tribological behaviour of protic ionic liquids with ammonium salts modified LABSA as lubricants and additives
Authors: Zhao Qin , Zhao Gaiqing , Zhang Ming , Wang Xiaobo , Liu Weimin 
Published: October 2012

ABSTRACTTwo typical protic ionic liquids with ammonium salts modified linear alkylbenzene sulfonic acid (LABSA) were synthesised, and their tribological behaviours used as both lubricants compared with PAO10 and additives in PAO10 were evaluated with an Optimol SRV‐I oscillating reciprocating friction and wear tester. The results show that the two novel protic ionic liquids with ammonium salts modified LABSA exhibited excellent friction‐reducing and anti‐wear properties as lubricants and can also significantly improve the tribological performance of the base fluids as friction modifier additives. The worn surfaces were characterised by scanning electron microscope and X‐ray photoelectron spectroscopy, indicating that the excellent anti‐wear and friction‐reducing performance could attributed to the boundary lubrication films that could contain both the tribochemical film composed of organic amine decomposed from the protic ionic liquids with ammonium salts and the stable chemical absorbed film through the interactions between the sulfonate anions and surface metallic atoms during the sliding process. Copyright © 2012 John Wiley & Sons, Ltd.