bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential encoding of mammalian proprioception by voltage-gated sodium channels
Authors: Espino Cyrrus M. , Nagaraja Chetan , Ortiz Serena , Dayton Jacquelyn R. , Murali Akash R. , Ma Yanki , Mann Emari L. , Garlapalli Snigdha , Wohlgemuth Ross P. , Brashear Sarah E. , Smith Lucas R. , Wilkinson Katherine A. , Griffith Theanne N. 
Published: August 2024

AbstractAnimals that require purposeful movement for survival are endowed with mechanosensory neurons called proprioceptors that provide essential sensory feedback from muscles and joints to spinal cord circuits, which modulates motor output. Despite the essential nature of proprioceptive signaling in daily life, the mechanisms governing proprioceptor activity are poorly understood. Here, we have identified distinct and nonredundant roles for two voltage-gated sodium channels (NaVs), NaV1.1 and NaV1.6, in mammalian proprioception. Deletion of NaV1.6 in somatosensory neurons (NaV1.6cKOmice) causes severe motor deficits accompanied by complete loss of proprioceptive transmission, which contrasts with our previous findings using similar mouse models to target NaV1.1 (NaV1.1cKO). In NaV1.6cKOanimals, loss of proprioceptive feedback caused non-cell- autonomous impairments in proprioceptor end-organs and skeletal muscle that were absent in NaV1.1cKOmice. We attribute the differential contribution of NaV1.1 and NaV1.6 in proprioceptor function to distinct cellular localization patterns. Collectively, these data provide the first evidence that NaV subtypes uniquely shape neurotransmission within a somatosensory modality.TeaserVoltage gated sodium channels differentially encode mammalian proprioception via distinct cellular localization patterns.
OrthoBrowser: Gene Family Analysis and Visualization
Authors: Hartwick Nolan T. , Michael Todd P. 
Published: August 2024

SummaryThe analysis of gene families across diverse species is pivotal in elucidating evolutionary dynamics and functional genomic landscapes. Typical analysis approaches often require significant computational expertise and user time. We introduce OrthoBrowser, a static site generator that will index and serve phylogeny, gene trees, multiple sequence alignments, and novel multiple synteny alignments. This greatly enhances the usability of tools like OrthoFinder by making the detailed results much more visually accessible. This interface can scale reasonably up to hundreds of genomes, allows a user to filter this large dataset to a subset of samples they are interested in at that particular moment in time, or “zoom in” to only a subtree of the orthogroup. The multiple synteny alignment method uses a progressive hierarchical alignment approach in the protein space using orthogroup membership to establish orthology. Orthobrowser makes it easy for users to identify, interact with, explore, and share key information about their gene families of interest.AvailabilityOrthoBrowser is available under the MIT license at:https://gitlab.com/salk-tm/orthobrowserContactnhartwick@salk.eduSupplementary InformationExample OrthoBrowser Results:https://orthobrowserexamples.netlify.app/
The Human Cerebellum: A Digital Anatomical Atlas at the Level of Individual Folia
Authors: Samuelsson John G. , Schmahmann Jeremy D. , Sereno Martin , Rosen Bruce , Hämäläinen Matti S. 
Published: August 2024

AbstractScientific interest in the cerebellum has surged in the last few decades with an emerging consensus on a multifaceted functionality and intricate, but not yet fully understood, functional topography over the cerebellar cortex. To further refine this structure-function relationship and quantify its inter-subject variability, a high-resolution digital anatomical atlas is fundamental. Using a combination of manual labeling and image processing, we turned a recently published reconstruction of the human cerebellum, the first such reconstruction fine enough to resolve the individual folia, into a digital atlas with both surface and volumetric representations. Its unprecedented granularity (0.16 mm) and detailed expert labeling make the atlas usable as an anatomical ground truth, enabling new ways of analyzing and visualizing cerebellar data through its digital format.
MYC shapes ER-mitochondria calcium transfer by directly targetingITPR1: implications for MYC-induced safeguard mechanisms and cancer
Authors: Ma Xingjie , Tsalikis Athanasios , Vernier Mathieu , Zhu Kexin , El Hassani Jéhanne , Ziegler Dorian , Huna Anda , Coquet Claire , Revechon Gwladys , Margand Céline , Benard Flavie , Czarnecka-Herok Joanna , Di Cacito Isabelle Iacono , Koering Catherine , Attignon Valéry , Carrere Marjorie , Jollivet Florence , Mollereau Bertrand , Vanbelle Christophe , Gandrillon Olivier , Dumont Benoît , Delloye-Bourgeois Céline , Hernandez-Vargas Hector , Broutier Laura , Martin Nadine , Bernard David 
Published: August 2024

ABSTRACTThe MYC and NMYC transcription factors (TFs) play a key role in cell proliferation and are overexpressed in most cancer cells. However, in normal cells their overexpression triggers safeguard mechanisms promoting cell death and cellular senescence, which are bypassed in cancer cells. The mechanisms of action of this TF family are only partially understood. Here, we reveal that in normal cells MYC binds to the Inositol 1,4,5-Trisphosphate Receptor type 1 (ITPR1)gene and upregulates its expression, triggering an ER-mitochondria calcium (Ca2+) transfer, which is involved in MYC-induced cell death and senescence. Supporting a tumor suppressive role of MYC/ITPR1 axis,ITPR1expression is generally decreased in cancer and reactivation of this pathway induces cancer cell death. Nevertheless, some cancer cells, generally expressing high levels ofMYCNand/orMYC, also express high level ofITPR1, which correlates with high expression ofBCL2, encoding an inhibitor of ITPR1. Strikingly, in high-riskMYCN-amplified neuroblastoma,ITPR1expression is controlled by NMYC and its level correlates with worse patient survival. In these cells, blocking the interaction between BCL2 and ITPR1 induces mitochondrial Ca2+accumulation and cell death, and decreases tumor size. Collectively these data highlight a new function of MYC factors by controlling Ca2+signaling, which could constitute an unsuspected vulnerability for some cancer cells, including high-riskMYCN-amplified neuroblastoma cells.
Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution
Authors: Fuhrmann Falko , Nebeling Felix C. , Musacchio Fabrizio , Mittag Manuel , Poll Stefanie , Müller Monika , Giovannetti Eleonora Ambrad , Maibach Michael , Schaffran Barbara , Burnside Emily , Chan Ivy Chi Wai , Lagurin Alex Simon , Reichenbach Nicole , Kaushalya Sanjeev , Fried Hans , Linden Stefan , Petzold Gabor C. , Tavosanis Gaia , Bradke Frank , Fuhrmann Martin 
Published: August 2024

AbstractThe medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control.In vivorecordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photonin vivoimaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ±0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes’ motility (58.9 ±2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.
Innovations in Alginate Catabolism Leading to Heterotrophy and Adaptive Evolution of Diatoms
Authors: Lim Zeng Hao , Zheng Peng , Quek Christopher , Nowrousian Minou , Aachmann Finn L. , Jedd Gregory 
Published: August 2024

AbstractA major goal of evolutionary biology is to identify the genetic basis for the emergence of adaptive traits. Diatoms are ancestrally photosynthetic microalgae. However, in the genusNitzschia, loss of photosynthesis led to a group of free-living secondary heterotrophs whose manner of energy acquisition is unclear. Here, we sequence the genome of the non-photosynthetic diatomNitzschiasing1 and identify the genetic basis for its catabolism of the brown seaweed cell wall polysaccharide alginate.N. sing1 obtained an endolytic alginate lyase enzyme by horizontal gene transfer (HGT) from a marine bacterium. Subsequent gene duplication and transposition led to 91 genes in three distinct gene families. One family retains the ancestral endolytic enzyme function. By contrast, the two others underwent domain duplication, gain, loss, rearrangement, and mutation to encode novel functions that can account for oligosaccharide import through the endomembrane system and the exolytic production of alginate monosaccharides. Together, our results show how a single HGT event followed by substantial gene duplication and neofunctionalization led to alginate catabolism and access to a new ecological niche.HighlightsN. sing1 acquired an alginate lyase (ALY) gene by horizontal gene transfer from a marine bacteriumThis founding gene expanded and diversified to comprise 3 major families across 30 lociDerived functions account for alginate import and processing into monomersDomain duplication, gain, loss, mutation, andde novosequence evolution underlie ALY gene neofunctionalizationGraphical Abstract
A remarkable degree of conformance between the visual streak of the Mongolian gerbil and the human central retina
Authors: Günter Alexander , Jarboui Mohamed Ali , Mühlfriedel Regine , Seeliger Mathias W. 
Published: August 2024

AbstractThe Mongolian gerbil (MG) is a day-active rodent that lives in desert-like environments and thus very much relies on vision. To allow for a particularly exact view of the horizon, the region of highest visual acuity, the visual streak (VS), forms a horizontal band across the retina between the projection areas of the sky and the ground. Here, we assessed the retinal basis of this specialized region and compared the findings to the human central high-acuity region culminating in the macula. We found an increased density of cones, elongated photoreceptor outer segments (OSs) and a minimization of intersecting surface vessels to improve quality of vision, all of which is analogous to the human macula. Stunningly, also the area of retinal pigment epithelium (RPE) cells was significantly smaller in the VS region than in the periphery, again similar to what is found in the human macula. Our data therefore suggest that the remarkable degree of conformance between the VS of the MG and the human macula renders the MG a promising rodent, non-primate model of the central human retina.
OsWHY1/OsTRXz/OsMORFs complex is essential for RNA modification and early chloroplast development in rice
Authors: Zheng Xiangzi , Lu Qingzheng , Luo Yuling , Xu Jiaxuan , Wang Weiqi , Tan Min , Liao Dongmei , Hong Wuqiang , Chen Sirong , Lin Chuheng , Wang Xiaoli , Fan Chunlan , Habiba  , Wang Xiaowei , Li Yanyun , Zhang Yu , Lin Wenfang , Miao Ying 
Published: August 2024

AbstractWHIRLY (WHY) proteins are single-stranded DNA/RNA-binding proteins that play multifaceted roles in various plant species. The regulatory mechanisms of WHY proteins in rice remains blank. Here we demonstrate thatOsWHY1in rice is required for early chloroplast development. CRISPR/Cas9-generatedoswhy1knockout lines displayed albino seedling phenotypes, abnormal chloroplast structure and comprised redox balance in leaves. OsWHY1 interacts with multiple plastid proteins, including the thioredoxin OsTRXz and two multiple organellar RNA editing factors (OsMORF8 and OsMORF9) in chloroplasts. Accordingly, several plastid genes dependent on plastid-encoded RNA polymerase (PEP) in theoswhy1mutants were significantly depressed at both transcript and protein levels. The editing ofrps14transcripts and splicing ofrpl2,along with their protein expression, were defective in theoswhy1mutants. OsWHY1 exhibited RNA-binding activity, specifically binding torps14andrpl2precursor RNAs, which underscores its role as a post-transcriptional regulator essential for normal protein synthesis in chloroplasts. Loss-of- function mutants of eitherOsWHY1orOsMORF9andOsTRXzdisplayed albino phenotypes, disrupted H2O2homeostasis, and defective RNA processing inrps14andrpl2, suggesting the OsWHY1-OsTRXz-OsMORFs regulatory module is vital for maintaining chloroplast stability and integrity through its RNA-binding activity and its role in recruiting OsTRXz and OsMORFs to ensure proper RNA modification.One sentence summaryOsWHY1 is integral to chloroplast development in rice through its RNA-binding activity and its role in recruiting OsTRXz and OsMORFs to ensure proper RNA modification.
Anisotropic excitonic magnetism from discrete
C4
symmetry in
CeRhIn5
Authors: Brener D. J. , Mallo I. Rodriguez , Lane H. , Rodriguez-Rivera J. A. , Schmalzl K. , Songvilay M. , Guratinder K. , Petrovic C. , Stock C. 
Published: August 2024

Anisotropy in strongly correlated materials is a central parameter in determining the electronic ground state and is tuned through the local crystalline electric field. This is notably the case in the CeCoxRh1−xIn5 system where the ground-state wave function can provide the basis for antiferromagnetism and/or unconventional superconductivity. We develop a methodology to understand the local magnetic anisotropy and experimentally investigate with neutron spectroscopy applied to antiferromagnetic (TN=3.8K) CeRhIn5, which is isostructural to d-wave superconducting (Tc=2.3K) CeCoIn5. Through diagonalizing the local crystal field Hamiltonian with discrete tetragonal C4 point group symmetry and coupling these states with the random phase approximation, we find two distinct modes polarized along the crystallographic c and a−b planes, agreeing with experiment. The anisotropy and bandwidth, underlying the energy scale of these modes, are tuneable with a magnetic field which we use experimentally to separate in energy single and multiparticle excitations thereby demonstrating the instability of excitations polarized within the crystallographic a−b plane in CeRhIn5. We compare this approach to a Seff=12 parametrizations and argue for the need to extend conventional SU(2) theories of magnetic excitations to utilize the multilevel nature of the underlying crystal-field basis states constrained by the local point-group C4 symmetry.

Published by the American Physical Society
2024
Who gains favor with green investors amidst climate risk?
Authors: Feng Lingbing , Huang Dasen 
Published: August 2024

PurposeThis study aims to investigate the impact of climate risk disclosure by listed companies on the entry of green investors. It seeks to understand how proactive climate risk disclosure can attract green investment and the underlying mechanisms that facilitate this process.Design/methodology/approachTextual analysis is employed to assess the extent of climate risk disclosure in annual reports. The research constructs indicators for green investor entry and applies regression analysis to examine the relationship between climate risk disclosure and green investment, considering various mediating variables such as positive online news coverage, ESG scores, and corporate reputation.FindingsGreen investors are more likely to invest in companies with higher levels of climate risk disclosure. This relationship is robust across different types of firms, with non-state-owned, non-high-tech, large-scale firms, and those in the Eastern region showing a stronger attraction to green investors. Climate risk disclosure promotes green investment through the “signal transmission” mechanism, enhancing corporate reputation and ESG performance.Originality/valueThis paper extends the traditional theory of external incentives for corporate green development to include autonomous incentives through active climate risk disclosure. It provides new insights into the theory of corporate sustainable development and offers practical recommendations for enhancing corporate green development pathways. The study’s comprehensive approach and use of extensive data contribute valuable knowledge to the field of green investment and corporate sustainability.
Covid-19 pandemic trouble? An exploratory study on German acquisitions in Japan
Authors: Massmann Kevin , Bebenroth Ralf 
Published: August 2024

PurposeThis study investigated how the Covid-19 pandemic impacted cross-border acquisitions. Though literature suggests that cross-border investments decreased during the pandemic, there is little conclusive evidence on specific characteristics in the execution of particular acquisitions during such times. We applied the case study format to conduct our investigation by (1) providing a classification of influences on cross-border procedures and (2) highlighting critical characteristics during three phases of acquisitions, namely, search, negotiation and integration periods.Design/methodology/approachThe grounded theory approach was applied to three in-depth case studies of German companies that acquired Japanese targets during the Covid-19 pandemic. Data were supplemented by information received through additional case studies of German subsidiaries in Japan and interviews with consultants.FindingsFirms had already intended to acquire their respective targets, with their decisions having been made prior to the Covid-19 pandemic. Thus, the pandemic had no impact on target selection in the case firms. Owing to travel restrictions, information exchange was limited which inevitably led to higher usage of digitalization. While several barriers led to delays in negotiations, prevailing mutual trust and assistance from consultants helped to reduce difficulties. During the integration period, we found delays in synergy creation and increases in remote communication. Nevertheless, the digital workflow improved the efficiency.Originality/valueOur study provides novel insights into the execution of cross-border acquisitions impacted by the Covid-19 pandemic. We discuss new implications for mergers and acquisitions (M&A) research and practice for the post-pandemic era, focusing on German firms acquiring Japanese targets.
A grey target performance evaluation model for aeroengine pressure test bench
Authors: Zhang Yanhua , Ying Kaixin , Zhou Jialin , Cheng Yuehua , Xu Chenghui , Fang Zhigeng 
Published: August 2024

PurposeThis paper aims to optimize the air pressure regulation scheme of the aeroengine pressure test bench.Design/methodology/approachBased on the requirements of pressure regulation process and the operating mechanism of aeroengine pressure test bench, a grey performance evaluation index system is constructed. The combination of principal component analysis and grey theory is employed to assign weights to grey indexes. The grey target evaluation model is introduced to evaluate the performance of historical regulation processes, and the evaluation results are analyzed to derive optimization mechanism for pressure regulating schemes.FindingsA case study based on monitoring data from nearly 300 regulation processes verifies the feasibility of the proposed method. On the one hand, the improved principal component analysis method can achieve rational weighting for grey indexes. On the other hand, the method comparison intuitively shows that the proposed method performs better.Originality/valueThe pressure test bench is a fundamental technical equipment in the aviation industry, serving the development and testing of aircraft engines. Due to the complex system composition, the pressure and flow adjustment of the test bench heavily rely on manual experience, leading to issues such as slow adjustment speed and insufficient accuracy. This paper proposes a performance evaluation method for the regulation process of pressure test bench, which can draw knowledge from historical regulation processes, provide guidance for the pressure regulation of test benches, and ultimately achieve the goal of reducing equipment operating costs.
Design and experiment of CLIBOT, a novel UHV insulator climbing robot with discrete optimization
Authors: Li Yunxiang , Ai Yunfei , Zou Jinzhou , Liu Liangyu , Liu Chengjian , Fu Siheng , Zou Dehua , Wei Wang 
Published: August 2024

Purpose
By analyzing the shortcomings of existing insulator robots, a novel ultra high voltage (UHV) insulator climbing robot, which could transfer between adjacent insulator strings, is proposed for operation on 800KV multiple-string insulators. An extended inchworm-like configuration was chosen and a stable gripping claw suitable for the insulator string was designed to enable the robot to multiple-string insulators. Then a set of nonheuristic structural parameters that can influence energy consumption was chosen to formulate a nonlinear optimization problem based on the configuration, which improved the energy efficiency of the robot during progressing along a string of insulator.

Design/methodology/approach
The purpose of this paper is to design an insulator climbing robot for operation on UHV multiple-string insulators, which could transfer between adjacent insulator strings and progressed along a string of insulator with high energy efficiency.

Findings
A physical prototype was constructed that can operate at the speed of six pieces per minute (approximately 1.44 meters per minute) on a single string and complete transference between adjacent strings in 45 s. The energy consumption of joints during progressed along a string of insulator had been reduced by 38.8% with the optimized parameters, demonstrating the consistency between the experimental and simulation results.

Originality/value
An insulator climbing robot for operation on UHV multiple-string insulators has been developed with energy consumption optimization design. The robot can transfer between adjacent insulator strings and progressed along a string of insulator with high energy efficiency. The CLIBOT could be expanded to detect or clean the insulators with similar specification.
Board reforms and managerial risk-taking incentives: evidence from Chinese central state-owned enterprises
Authors: Hu Shiyang , Wei Chunyan , Xue Rui , Yin Liang , Zhu Bo 
Published: August 2024

PurposeThis paper examines the effect of board reforms on managerial risk-taking incentive provision in state-owned enterprises (SOEs) whose managers are undue risk-averse.Design/methodology/approachWe use the staggered implementation of board reforms in Chinese central government-controlled state-owned enterprises (CSOEs) as an exogenous shock to board governance. We collect data on board reforms for a set of pilot CSOEs during the period 2005 to 2020 from the State-owned Assets Supervision and Administration Commission (SASAC) website by hand. We use a generalized difference-in-difference (DID) design to test the effect of staggered board reform adoption on managerial risk-taking incentive provision.FindingsWe find a positive relationship between board reforms and risk-taking inventive provision, i.e. pay-performance sensitivity, promotion-performance sensitivity and performance target difficulty. The documented relationship is stronger when the value of risk-taking is higher. We also find that board reforms lead to greater risky but value-enhancing investments and that managerial risk-taking incentive provision acts as an important channel through which board reforms improve value-enhancing risk-taking.Originality/valueOur findings suggest that board reforms that improve board governance are effective in addressing risk-related agency conflicts in emerging markets. The findings also highlight the importance of managerial risk-taking incentive provision in inducing risky but value-enhancing investments.