bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primate-Specific miR-663 Functions as a Tumor Suppressor by Targeting PIK3CD and Predicts the Prognosis of Human Glioblastoma
Authors: Shi Yu , Chen Cong , Zhang Xia , Liu Qing , Xu Jin-Ling , Zhang Hua-Rong , Yao Xiao-Hong , Jiang Tao , He Zhi-Cheng , Ren Yong , Cui Wei , Xu Chuan , Liu Ling , Cui You-Hong , Yu Shi-Zhu , Ping Yi-Fang , Bian Xiu-Wu 
Published: February 2014

Abstract
Purpose: To determine the prognostic significance of miR-663 in glioblastoma, its effect in tumor progression, and the underlying mechanism.
Experimental Design: Specimens from 256 cases of patients with glioma, including 239 patients with follow-up information, were used to analyze the association between miR-663 and patients' prognosis by Kaplan–Meier and multivariate Cox regression analyses. The effects of miR-663 on glioblastoma cell proliferation and invasion were examined both in vitro and in vivo. Bioinformatics prediction and signal network analysis were applied to identify the putative targets of miR-663, which were further verified by luciferase reporter assay, rescue experiments as well as the immunohistochemistry (IHC) and Western blotting examination of downstream effectors. Quantitative reverse transcriptase PCR (qRT-PCR) and IHC were applied to investigate the clinical association between miR-663 and its target in human glioblastoma specimens.
Results: miR-663 was inversely correlated with glioma grades but positively correlated with patients' survival. Furthermore, two distinct subgroups of patients with glioblastoma with different prognoses were identified on the basis of miR-663 expression in our specimens and that from The Cancer Genome Atlas (TCGA) database. Overexpression of miR-663 significantly suppressed the proliferation and invasion of glioblastoma cells in vitro and in vivo. Mechanistically, we discovered PIK3CD as a direct target of miR-663 and found that phosphorylated AKT and three key downstream effectors of PIK3CD, i.e., CCND1, MMP2, and MMP7, were downregulated by miR-663 overexpression. Moreover, PIK3CD was inversely correlated with miR-663 in glioblastoma specimens and predicted poor prognosis of patients with glioblastoma.
Conclusion: miR-663 is a novel prognostic biomarker and a potential therapeutic candidate for glioblastoma. Clin Cancer Res; 20(7); 1803–13. ©2014 AACR.
Interferon lambda 3 rs12979860 polymorphism in patients with haemophilia and HCV infection: a predictor of spontaneous viral clearance and sustained virological response
Authors: Linari Silvia , Aghemo Alessio , Bartolozzi Dario , Santagostino Elena , Rumi Maria Grazia , Fognani Elisa , Fasulo Maria Rosaria , Gragnani Laura , Bruno Raffaele , Morfini Massimo , Zignego Anna Linda , Colombo Massimo , Mancuso Maria Elisa 
Published: February 2014

SummaryChronic hepatitis C is the main cause of morbidity and mortality in adult haemophilic patients who received non-virally inactivated plasma-derived clotting factor concentrates. Overall, spontaneous viral clearance rate is 10–25% and the only approach that can halt disease progression is hepatitis C virus (HCV) eradication by means of antiviral therapy. In non-haemophilic patients a single nucleotide polymorphism located upstream the gene of interferon lambda 3 (IFNλ3) has been associated with both spontaneous viral clearance and sustained virological response after antiviral treatment. The aim of this study was to assess whether the rs12979860 polymorphism was a predictor of spontaneous viral clearance and of sustained virological response after antiviral therapy in a large cohort of haemophilic patients with HCV infection. The rs12979860 polymorphism, defined as CC genotype or T allele, was tested in a cohort of 342 haemophilic patients and evaluated as predictor of spontaneous clearance or response to antiviral therapy. By multivariate regression analysis the IFNλ3 CC genotype was an independent predictor of spontaneous viral clearance (odds ratio: 3.7, 95% confidence interval: 2.0–6.8). Sustained virological response rates were doubled in patients with the CC genotype than in those with the T allele (78% vs 44%; p<0.001), especially in patients with HCV type 1 (67% vs 32%; p<0.001) and higher sustained response rates were observed in patients with the CC genotype who did not achieve rapid virological response (61% vs 30% in T allele patients; p=0.006).
Radiofrequency Ablation Versus Antiarrhythmic Medication for Treatment of Ventricular Premature Beats From the Right Ventricular Outflow Tract
Authors: Ling Zhiyu , Liu Zengzhang , Su Li , Zipunnikov Vadim , Wu Jinjin , Du Huaan , Woo Kamsang , Chen Shaojie , Zhong Bin , Lan Xianbin , Fan Jinqi , Xu Yanping , Chen Weijie , Yin Yuehui , Nazarian Saman , Zrenner Bernhard 
Published: February 2014

Background—
The purpose of this study was to compare the efficacy of radiofrequency catheter ablation (RFCA) versus antiarrhythmic drugs (AADs) for treatment of patients with frequent ventricular premature beats (VPBs) originating from the right ventricular outflow tract (RVOT).

Methods and Results—

A total of 330 eligible patients were included in the study and were randomly assigned to RFCA or AADs group. The absolute number and the burden of VPBs on 12-lead Holter monitors were measured at baseline and at 1st, 3rd, 6th, and 12th months after randomization. Left ventricular eject fraction was evaluated by transthoracic echocardiogram at baseline and at 3 and 6 months after randomization. During the 1-year follow-up period, VPB recurrence was significantly lower in patients randomized to RFCA group (32 patients, 19.4%) versus AADs group (146 patients, 88.6%;
P
<0.001, log-rank test). In a Poisson generalized estimating equations (GEE) regression model, RFCA was associated with a greater decrease in the burden of VPBs (incidence rate ratio 0.105; 95% confidence intervals [0.104–0.105];
P
<0.001) compared with AADs. In a liner GEE model, the left ventricular eject fraction had a tendency to increase after the treatment in both groups (coefficient, 0.584; 95% confidence intervals [0.467–0.702];
P
<0.001). In a Cox proportional model, the QS morphology in lead I was the only predictor of VPB recurrence free for catheter ablation (hazards ratio, 0.154; 95% confidence intervals [0.044–0.543];
P
=0.004).

Conclusions—
Catheter ablation is more efficacious than AADs for preventing VPB recurrence in patients with frequent VPBs originating from the RVOT. QS morphology in lead I was associated with better outcome after ablation.
Risk for Myocardial Infarction and Stroke After Community-Acquired Bacteremia
Authors: Dalager-Pedersen Michael , Søgaard Mette , Schønheyder Henrik Carl , Nielsen Henrik , Thomsen Reimar Wernich 
Published: February 2014

Background—
Infections may trigger acute cardiovascular events, but the risk after community-acquired bacteremia is unknown. We assessed the risk for acute myocardial infarction and ischemic stroke within 1 year of community-acquired bacteremia.

Methods and Results—
This population-based cohort study was conducted in Northern Denmark. We included 4389 hospitalized medical patients with positive blood cultures obtained on the day of admission. Patients hospitalized with bacteremia were matched with up to 10 general population controls and up to 5 acutely admitted nonbacteremic controls, matched on age, sex, and calendar time. All incident events of myocardial infarction and stroke during the following 365 days were ascertained from population-based healthcare databases. Multivariable regression analyses were used to assess relative risks with 95% confidence intervals (CIs) for myocardial infarction and stroke among bacteremia patients and their controls. The risk for myocardial infarction or stroke was greatly increased within 30 days of community-acquired bacteremia: 3.6% versus 0.2% among population controls (adjusted relative risk, 20.86; 95% CI, 15.38–28.29) and 1.7% among hospitalized controls (adjusted relative risk, 2.18; 95% CI, 1.80–2.65). The risks for myocardial infarction or stroke remained modestly increased from 31 to 180 days after bacteremia in comparison with population controls (adjusted hazard ratio, 1.64; 95% CI, 1.18–2.27), but not versus hospitalized controls (adjusted hazard ratio, 0.95; 95% CI, 0.69–1.32). No differences in cardiovascular risk were seen after >6 months. Increased 30-day risks were consistently found for a variety of etiologic agents and infectious foci.

Conclusions—
Community-acquired bacteremia is associated with increased short-term risk of myocardial infarction and stroke.
Transient Ischemic Attack Requiring Hospitalization of Children in the United States
Authors: Adil Malik M. , Qureshi Adnan I. , Beslow Lauren A. , Jordan Lori C. 
Published: February 2014

Background and Purpose—
Transient ischemic attacks (TIA) are not well described in children. We assessed the prevalence of risk factors for TIA requiring hospitalization in children in a large national database.

Methods—

Using the Healthcare Cost and Utilization Project Kids’ Inpatient Database, children aged 1 to 18 years admitted for TIA in 2003, 2006, and 2009 were identified by
International Classification of Diseases, Ninth Revision, Clinical Modification
code 435. Descriptive analyses identified patient characteristics. Trend analysis determined the change in annual average hospitalization days from 2003 to 2009.

Results—

TIA was the primary diagnosis for 531 children. Important secondary diagnoses included sickle cell disease (20%), congenital heart disease (11%), migraine (12%), moyamoya disease (10%), and stroke (4%). Mean length of stay decreased from 3.0 days (95% confidence interval, 2.4–3.6) in 2003 to 2.3 days (95% confidence interval, 2.0–2.7) in 2009 (
P
=0.04). During the same period, 2590 children were admitted with ischemic stroke; 4.8 children with stroke were admitted for every child with TIA.

Conclusions—
Recognized risk factors for TIA, including sickle cell disease, congenital heart disease, moyamoya, recent stroke, and migraine, were present in <60% of children. Pediatric admissions for ischemic stroke were ≈5-fold more common than for TIA. Further study is required to understand the risk of stroke after TIA in children to guide appropriate evaluation and treatment.
Variation in Do-Not-Resuscitate Orders for Patients With Ischemic Stroke
Authors: Kelly Adam G. , Zahuranec Darin B. , Holloway Robert G. , Morgenstern Lewis B. , Burke James F. 
Published: February 2014

Background and Purpose—
Decisions on life-sustaining treatments and the use of do-not-resuscitate (DNR) orders can affect early mortality after stroke. We investigated the variation in early DNR use after stroke among hospitals in California and the effect of this variation on mortality-based hospital classifications.

Methods—
Using the California State Inpatient Database from 2005 to 2011, ischemic stroke admissions for patients aged ≥50 years were identified. Cases were categorized by the presence or the absence of DNR orders within the first 24 hours of admission. Multilevel logistic regression models with a random hospital intercept were used to predict inpatient mortality after adjusting for comorbidities, vascular risk factors, and demographics. Hospital mortality rank order was assigned based on this model and compared with the results of a second model that included DNR status.

Results—
From 355 hospitals, 252 368 cases were identified, including 33 672 (13.3%) with early DNR. Hospital-level–adjusted use of DNR varied widely (quintile 1, 2.2% versus quintile 5, 23.2%). Hospitals with higher early DNR use had higher inpatient mortality because inpatient mortality more than doubled from quintile 1 (4.2%) to quintile 5 (8.7%). Failure to adjust for DNR orders resulted in substantial hospital reclassification across the rank spectrum, including among high mortality hospitals.

Conclusions—
There is wide variation in the hospital-level proportion of ischemic stroke patients with early DNR orders; this variation affects hospital mortality estimates. Unless the circumstances of early DNR orders are better understood, mortality-based hospital comparisons may not reliably identify hospitals providing a lower quality of care.
Late Winter Sea Ice in the Bering Sea: Predictor for Maize and Rice Production in Northeast China
Authors: Zhou Mengzi , Wang Huijun 
Published: February 2014

AbstractIn this research, the linkage between late winter Bering Sea ice and maize/rice yields in northeastern China (NEC) is investigated. Results show that such ice influences NEC crop production and thus can be employed to predict harvest amounts. Further investigation reveals that positive anomalies of late winter sea ice cover can persist until spring and that spring sea ice can strengthen North Pacific Oscillation (NPO) positive-phase patterns, and vice versa. NPO significantly affects sea surface temperature (SST) over the North Pacific Ocean through sea–air interaction—in particular, in the Kuroshio region—that may persist until summer. In association with the positive SST anomalies, the polar vortex weakens and the western Pacific subtropical high strengthens, resulting in the convergence of southern and northern air masses over NEC. Moreover, both the southerly flow along the western flank of the western Pacific subtropical high and the easterly flow from the Japan Sea and the central Pacific region supply more water vapor transport; thus, an anomalous water vapor convergence center appears in NEC. With the anomalous updrafts, NEC exhibits positive precipitation anomalies. The greenhouse effect of water vapor results in an increase in minimum temperature, thereby leading to a decrease in diurnal temperature range (DTR). This increase in minimum temperature and decrease in DTR are primary factors favoring increases in rice and maize yields, respectively.
Monthly averaged-hourly solar diffuse radiation model for the UK
Authors: Muneer T , Etxebarria S , Gago EJ 
Published: February 2014

Monthly-averaged daily global irradiation data are now easily available from NASA website. Using established models it is then possible to decompose the daily to averaged-hourly global irradiation. The missing link so far has been hourly averaged diffuse irradiation. In this article data was pooled from 10 UK locations to obtain a regression model to complete the above missing link. It was presently shown that the averaged-data regressions are distinctly different from previously available hour-by-hour regressions. Practical application: The present work has explored a correlation between averaged-hourly diffuse and global irradiation using the diffuse ratio–clearness index envelope. Results show that a strong regression relationship is thus obtainable. The present work has therefore the potential for other similar research that may be followed up in a likewise manner.
Integrating contrast-enhanced sonography in the follow-up algorithm of hepatocellular carcinoma treated with radiofrequency ablation: single cancer center experience
Authors: Catalano Orlando , Izzo Francesco , Vallone Paolo , Sandomenico Fabio , Albino Vittorio , Nunziata Antonio , Fusco Roberta , Petrillo Antonella 
Published: February 2014

Background Hepatocellular carcinoma (HCC) patients treated with percutaneous ablation require close follow-up for early detecting and treating tumor recurrence. Purpose To illustrate our single-center prospective experience on using contrast-enhanced ultrasound (CEUS) as a follow-up tool alternated with computed tomography (CT). Material and Methods In a 7-year period 588 patients with one to three HCCs were treated with radiofrequency ablation (alone or combined with ethanol injection). Patients with completely ablated tumors at 1-month CT scan were followed up serially, using alternated CEUS (one microbubbles injection per lobe) and CT every 3 months for 2 years. In few cases magnetic resonance imaging (MRI) was employed instead of or in addition to CT. The following patterns of recurrence were considered: A, enhancing tissue within the lesion; B, enhancing tissue adherent to the lesion; C, enhancing tissue within the same liver segment of the treated nodule; and D, enhancing tissue within a different segment. Patients with positive CEUS underwent confirmatory CT/MRI (standard reference). Results Median follow-up was 19 months. There were 221 recurrences. Three pattern A recurrences (2 detected by CEUS and 1 by CT), 86 pattern B recurrences (44 detected by CEUS and 42 by CT), 70 pattern C recurrences (32 detected by CEUS and 38 by CT), and 62 pattern D recurrences (23 detected by CEUS and 39 by CT). CT detected additional nodules in 16/101 patients with positive CEUS. Conclusion CEUS follow-up of HCC patients after ablation is feasible. Since 72% recurrences develop in the same segment of the necrotic nodule, CEUS proves to be effective despite the minor visualization of the entire liver during the arterial phase when compared to CT and MRI. Including CEUS in patient follow-up may reduce the number of CT and MRI examinations.
Pilot study of a high-frequency school-based hearing screen to detect adolescent hearing loss
Authors: Sekhar Deepa L , Zalewski Thomas R , Ghossaini Soha N , King Tonya S , Rhoades Julie A , Czarnecki Beth , Grounds Shannon , Deese Barry , Barr Ashley L , Paul Ian M 
Published: February 2014

Objective Like most of the United States, school-based hearing screening in Pennsylvania focuses on low-frequency, conductive hearing losses typical for young children, rather than the high-frequency, noise-induced hearing loss more prevalent among adolescents. The objective of this study was to compare the sensitivity and specificity of current school hearing screening in Pennsylvania with hearing screening including high frequencies, designed to detect adolescent hearing loss. Setting A single public high school. Methods In the Autumn of 2011 the high-frequency screen was delivered alongside the Pennsylvania school screen for students in the 11th grade. Screening referrals and a subset of passes returned for “gold standard” testing with audiology in a sound treated booth, in order to determine the sensitivity and specificity of the screening tests. Results Of 282 participants, five (2%) were referred on the Pennsylvania school screen, and 85 (30%) were referred on the high-frequency screen. Of the 48 who returned for gold standard testing with audiology, hearing loss was diagnosed in 9/48 (19%). Sensitivity of the Pennsylvania and high-frequency screens were 13% (95% confidence interval [CI] 0–53%) and 100% (95% CI 66–100%) respectively. Specificity of the Pennsylvania and high-frequency screens were 97% (95% CI 87–100%) and 49% (95% CI 32–65%) respectively. Conclusions Current school hearing screens have low sensitivity for detection of adolescent hearing loss. Modifying school-based protocols may be warranted to best screen adolescents, and make optimal use of school nurse time and effort.
Atypical Stress-Avulsion Fracture of the Lisfranc Joint Complex
Authors: O’Neill Barry J. , Sweeney Laura A. , Moroney Paul J. , Mulhall Kevin J. 
Published: February 2014

Antiphospholipid syndrome and systemic erythematosus have been associated with metatarsal stress fractures. Stress fractures of the Lisfranc joint complex are uncommon injuries but have been reported to occur most frequently in ballet dancers. We present a case of an avulsion fracture of the Lisfranc joint complex that occurred spontaneously. We have reviewed the association between systemic conditions and metatarsal fractures and proposed a series of hypothetical pathological events that may have contributed to this unusual injury. Level of Evidence: Therapeutic, Level IV: Case report
Howling at the moon? The effect of lunar phases on post-surgical pain outcome
Authors: Komann Marcus , Weinmann Claudia , Meissner Winfried 
Published: February 2014

Objective: Many people are convinced that lunar phases influence their lives – despite the fact that a lot of studies have shown that this belief is wrong. In this article, we investigate the effect of lunar phases on acute post-surgical pain and on treatment-related side effects. We hypothesize that there is no influence. Methods: The data for the study were collected in 2010 and 2011 in 10 international hospitals participating in the research project PAIN OUT. Hospitalized patients were asked for their pain after surgery and pain treatment side effects using numerical ratings scales from 0 to 10. We applied Kurskal–Wallis H-tests to find out if the four moon phases show significant differences in 14 outcome variables. Afterwards, we adjusted for age, gender and three tracer surgeries. Results: A total of 12,224 patient data sets were assessed. For most variables and sub-groups, there is no lunar effect on the observed outcome variables. The only items that show statistically significant differences are pain interference with sleep ( p = 0.01) and drowsiness ( p = 0.01). The only sub-groups that show statistically significant connections to lunar phases in some variables are men (7 out of 14 variables significant) and elderly people (4 out of 14 variables significant). Discussion: Even in the statistically significant sub-groups, the differences are small and only show up in some variables. We conclude that lunar phases have no effect on post-surgical pain or its side effects. The hypothesis holds. Thus, there is no reason for patients to postpone surgeries or to fear surgeries on any given date.
ADAP interactions with talin and kindlin promote platelet integrin αIIbβ3 activation and stable fibrinogen binding
Authors: Kasirer-Friede Ana , Kang Jian , Kahner Bryan , Ye Feng , Ginsberg Mark H. , Shattil Sanford J. 
Published: February 2014

Key PointsADAP interacts with talin and kindlin-3 in platelets. ADAP is a hematopoietic component of the molecular machinery that promotes activation of and stable fibrinogen binding to αIIbβ3.
A phase 1 clinical trial of long-term, low-dose treatment of WHIM syndrome with the CXCR4 antagonist plerixafor
Authors: McDermott David H. , Liu Qian , Velez Daniel , Lopez Lizbeeth , Anaya-O’Brien Sandra , Ulrick Jean , Kwatemaa Nana , Starling Judy , Fleisher Thomas A. , Priel Debra A. Long , Merideth Melissa A. , Giuntoli Robert L. , Evbuomwan Moses O. , Littel Patricia , Marquesen Martha M. , Hilligoss Dianne , DeCastro Rosamma , Grimes George J. , Hwang Samuel T. , Pittaluga Stefania , Calvo Katherine R. , Stratton Pamela , Cowen Edward W. , Kuhns Douglas B. , Malech Harry L. , Murphy Philip M. 
Published: February 2014

Key Points
Plerixafor can be given safely to WHIM syndrome patients twice daily for a 6-month period and appears promising as a treatment.
Novel FV mutation (W1920R, FVNara) associated with serious deep vein thrombosis and more potent APC resistance relative to FVLeiden
Authors: Nogami Keiji , Shinozawa Keiko , Ogiwara Kenichi , Matsumoto Tomoko , Amano Kagehiro , Fukutake Katsuyuki , Shima Midori 
Published: February 2014

Key PointsFVNara (W1920R), associated with serious deep vein thrombosis, is more resistant to APC relative to FVLeiden (R506Q). This mechanism results from significant decreases in FVa susceptibility to APC and FV cofactor activity for APC.
Cooperativity of RUNX1 and CSF3R mutations in severe congenital neutropenia: a unique pathway in myeloid leukemogenesis
Authors: Skokowa Julia , Steinemann Doris , Katsman-Kuipers Jenny E. , Zeidler Cornelia , Klimenkova Olga , Klimiankou Maksim , Ünalan Murat , Kandabarau Siarhei , Makaryan Vahagn , Beekman Renee , Behrens Kira , Stocking Carol , Obenauer Julia , Schnittger Susanne , Kohlmann Alexander , Valkhof Marijke G. , Hoogenboezem Remco , Göhring Gudrun , Reinhardt Dirk , Schlegelberger Brigitte , Stanulla Martin , Vandenberghe Peter , Donadieu Jean , Zwaan C. Michel , Touw Ivo P. , van den Heuvel-Eibrink Marry M. , Dale David C. , Welte Karl 
Published: February 2014

Key PointsCN/AML patients have a high frequency of CSF3R and RUNX1 mutations. CSF3R and RUNX1 mutations induce elevated proliferation of CD34+ cells.