bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromatographic fingerprinting of ipratropium and fenoterol in their novel co-formulated inhaler treating major respiratory disorders; application to delivered dose uniformity testing along with greenness and whiteness assessment
Authors: Ali Salma N. , Saad Samah S. , Fayed Ahmed S. , Marzouk Hoda M. 
Published: August 2024

AbstractIpratropium bromide (IPR) and fenoterol hydrobromide (FEN) have recently been combined in a promising inhaler to treat two prevalent inflammatory illnesses of the airways: bronchial asthma and chronic obstructive pulmonary disease (COPD). The necessity for a single, sensitive, and trustworthy analytical approach to cover the diverse and necessary tests of in-vitro and in-vivo studies is greatly grown with the rising production of new fixed combinations. Two novel, selective and environmentally friendly LC techniques were developed in order to guarantee precise measurement of IPR and FEN in their challenging formulation. The initial technique involved high-performance thin-layer chromatography (HPTLC) in conjunction with densitometric quantification. Chromatographic separation was attained on HPTLC plates utilizing ethyl acetate - ethanol - acetic acid (5.0:5.0:0.1, by volume) as a developing system. Densitometric quantification of the separated bands was carried out at 220.0 nm over concentration ranges of 0.50–15.0 µg/band for IPR and 0.50–12.0 µg/band for FEN. High-performance liquid chromatography (HPLC) paired with diode array detection (DAD) was the core of the second technique. The optimized separation was achieved on a Zorbax SB C18 (150 × 4.6 mm, 5 μm) column with a combination of 10.0 mM potassium dihydrogen orthophosphate, pH 5.0 ± 0.1, adjusted with o-phosphoric acid and methanol (70:30, v/v) as the mobile phase and pumped at flow rate of 1.0 mL/min. The peaks were monitored at 220.0 nm using diode array detection, achieving linearity range of 5.0–200.0 µg/mL for both drugs. The ICH criteria have been verified and both methods have been confirmed to be valid, and successfully applied for assay the cited drugs in the Atrovent® comp HFA metered dose inhaler as well as delivered dose uniformity testing of the final product. Finally, whiteness appraisal and several state-of-the-art green evaluation metrics were applied to evaluate the sustainability of the proposed methods. The suggested approaches produced promising results and are the first simple and sustainable methodologies for the simultaneous quantification of both drugs in different real samples, all of which strongly suggest their application in quality control laboratories.
First detection of kdr L1014F allele in Anopheles ziemanni and Anopheles pharoensis in Cameroon and distribution of the allele in members of the Anopheles gambiae complex
Authors: Mayi Marie Paul Audrey , Antonio-Nkondjio Christophe , Bamou Roland , Damiani Claudia , Cappelli Alessia , Djiappi-Tchamen Borel , Djamouko-Djonkam Landre , Ilbeigi Khamseh Nejad Mahdokht , Pichler Verena , Ricci Irene , Favia Guido 
Published: August 2024

Abstract
Background
Knockdown resistance (kdr) is one of the primary resistance mechanisms present in anopheline species. Although this mutation is largely spread across the Anopheles gambiae s.l. members, its prevalence in other species is still not well documented.

Methods
The present study investigated the distribution and allelic frequencies of kdr in An. gambiae s.l., An. pharoensis, and An. ziemanni samples collected in 2022 and 2023 in nine sites spread across five ecogeographical settings in Cameroon. Members of the An. gambiae complex were identified molecularly by polymerase chain reaction (PCR). kdr L1014F and L1014S alleles were screened by PCR and confirmed by sequencing.

Results
An. gambiae (49.9%), An. coluzzii (36.5%), and An. arabiensis (13%) were identified, and the frequency of the kdr L1014F was high in both An. gambiae and An. coluzzii in all sites. The kdr L1014F allele was detected for the first time in 8 out of 14 An. ziemanni samples examined and in 5 out of 22 An. pharoensis samples examined. The kdr L1014S allele was scarce and found only in the heterozygote “RS” state in An. arabiensis and An. gambiae in Yangah and Santchou.

Conclusions
The present study sheds light on the rapid expansion of the kdr L1014F allele in malaria vectors in Cameroon and stresses the need for surveillance activities also targeting secondary malaria vectors to improve the control of malaria transmission.

Graphical Abstract
The role of hexokinases in epigenetic regulation: altered hexokinase expression and chromatin stability in yeast
Authors: Karri Srinivasu , Dickinson Quinn , Jia Jing , Yang Yi , Gan Haiyun , Wang Zhiquan , Deng Yibin , Yu Chuanhe 
Published: August 2024

Abstract
Background
Human hexokinase 2 (HK2) plays an important role in regulating Warburg effect, which metabolizes glucose to lactate acid even in the presence of ample oxygen and provides intermediate metabolites to support cancer cell proliferation and tumor growth. HK2 overexpression has been observed in various types of cancers and targeting HK2-driven Warburg effect has been suggested as a potential cancer therapeutic strategy. Given that epigenetic enzymes utilize metabolic intermediates as substrates or co-factors to carry out post-translational modification of histones and nucleic acids modifications in cells, we hypothesized that altering HK2 expression could impact the epigenome and, consequently, chromatin stability in yeast. To test this hypothesis, we established genetic models with different yeast hexokinase 2 (HXK2) expression in Saccharomyces cerevisiae yeast cells and investigated the effect of HXK2-dependent metabolism on parental nucleosome transfer, a key DNA replication–coupled epigenetic inheritance process, and chromatin stability.

Results
By comparing the growth of mutant yeast cells carrying single deletion of hxk1Δ, hxk2Δ, or double-loss of hxk1Δ hxk2Δ to wild-type cells, we firstly confirmed that HXK2 is the dominant HXK in yeast cell growth. Surprisingly, manipulating HXK2 expression in yeast, whether through overexpression or deletion, had only a marginal impact on parental nucleosome assembly, but a noticeable trend with decrease chromatin instability. However, targeting yeast cells with 2-deoxy-D-glucose (2-DG), a clinical glycolysis inhibitor that has been proposed as an anti-cancer treatment, significantly increased chromatin instability.

Conclusion
Our findings suggest that in yeast cells lacking HXK2, alternative HXKs such as HXK1 or glucokinase 1 (GLK1) play a role in supporting glycolysis at a level that adequately maintains epigenomic stability. While our study demonstrated an increase in epigenetic instability with 2-DG treatment, the observed effect seemed to occur dependent on non-glycolytic function of Hxk2. Thus, additional research is needed to identify the molecular mechanism through which 2-DG influences chromatin stability.
Sonographic findings using the SAFE-A protocol in pre- and post-hemodialysis patients
Authors: Rabahi Matheus , Polito Maria Goretti , Takaoka Larissa Louise Cândida Pereira , Conte Marcus Barreto , Colares Philippe Figueiredo Braga 
Published: August 2024

Abstract
Background
Accurate assessment of relative intravascular volume is one of the cornerstones for the proper management of hospitalized patients requiring hemodialysis. Currently, the use of dynamic parameters such as bedside ultrasonography is recommended to support the assessment of the intravascular volume profile. This study aimed to prospectively evaluate findings of sonographic assessment of intravascular volume estimate (SAFE-A) protocol among hemodialysis inpatients with end-stage renal disease, before and after the hemodialysis sessions, and correlate these findings with the net ultrafiltrate (UFNET).

Results
A positive correlation was found between the negative variation of 1 point in the score of the SAFE-A protocol with the withdrawal of 426.73 mL of net ultrafiltrate.

Conclusions
There was a strong correlation between the score of the SAFE-A protocol and the net ultrafiltrate. Therefore, this study concludes that the application of the SAFE-A protocol in dialysis patients demonstrates a correlation between the suggested score and volume status, consistent with findings from the original study conducted in a distinct population.
Organoid as a promising tool for primary liver cancer research: a comprehensive review
Authors: Hu Xuekai , Wei Jiayun , Liu Pinyan , Zheng Qiuxia , Zhang Yue , Zhang Qichen , Yao Jia , Ni Jingman 
Published: August 2024

AbstractPrimary liver cancer (PLC) is one of the most common malignant gastrointestinal tumors worldwide. Limited by the shortage of liver transplantation donors and the heterogeneity of tumors, patients with liver cancer lack effective treatment options, which leads to rapid progression and metastasis. Currently, preclinical models of PLC fall short of clinical reality and are limited in their response to disease progression and the effectiveness of drug therapy. Organoids are in vitro three-dimensional cultured preclinical models with a high degree of heterogeneity that preserve the histomorphological and genomic features of primary tumors. Liver cancer organoids have been widely used for drug screening, new target discovery, and precision medicine; thus representing a promising tool to study PLC. Here, we summarize the progress of research on liver cancer organoids and their potential application as disease models. This review provides a comprehensive introduction to this emerging technology and offers new ideas for researchers to explore in the field of precision medicine.
Situational Judgement Tests among Palestinian community members and Red Crescent volunteers to inform humanitarian action: a cross-sectional study
Authors: Moussaoui L. S. , Quimby M. , Avancini H. , Salawdi A. , Skaik F. , Bani Odeh R. , Desrichard O. , Claxton N. 
Published: August 2024

Abstract
Background
Informing humanitarian action directly from community members is recognized as critical. However, collecting community insights is also a challenge in practice. This paper reports data collected among community members and Red Crescent volunteers in the occupied Palestinian territory. The aim was to test a data collection tool, situational judgment tests (SJTs), to collect insights in the community around three themes.

Methods
The SJTs covered violence prevention, road safety, and environmental pollution (waste), and were constituted of hypothetical scenarios to which respondents indicated how they would react. For each theme, the answers’ pattern provides insights for humanitarian action regarding which beliefs to address. A cross-sectional survey was conducted in January and February 2023 with 656 community members, and 239 Red Crescent volunteers.

Results
Data showed that violence is the theme for which the need is the highest among community members. Some responses varied according to the public (age, governorate, or disability level), suggesting actions could be tailored accordingly.

Conclusions
Despite many difficulties during data collection, this study show that the tool allowed to collect community insights, a crucial task to ensure adequate response to the challenges faced by community members and Red Crescent volunteers in occupied Palestine.
Causal relationships between baseball-team participation and academic performance among students
Authors: Lin Huei-Min , Huang Kai-Cheng , Tsai Ching-Chih 
Published: August 2024

AbstractThe understanding that students on varsity baseball teams exhibit lower academic performance amid the growth of baseball in Taiwan is well established. However, some studies have indicated a positive correlation between sports participation and academic achievement. Therefore, this study delved into the causal relationships between baseball-team participation and academic performance among ninth-grade students in Taiwan. It further explored the influence of various background factors based on the three facets of family capital on their academic performance. Data collected from an education longitudinal survey of secondary school students. Through bivariate analysis, factor analysis, and multiple regression analysis, the following findings emerged: (a) Baseball-team students exhibited 47.5 points lower academic performance than non-baseball-team ones. (b)The low academic performance was not exclusively a result of the poorer academic performance of indigenous students. (c)The hypothesis that three dimensions of family capital impact on academic performance was supported. (d) Baseball-team participation contributed to the low academic performance, which was not caused by the low eighth-grade results of the students. They exhibited low BCT scores after their 8th-grade results was controlled.
The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer
Authors: Jin Zhongkui , Xu Wang , Yu Kunlin , Luo Cailu , Luo Xiaodan , Lian Tao , Liu Changshan 
Published: August 2024

Abstract
Background
Circular RNAs (circRNAs) are capable of affecting breast cancer (BC) development. However, the role and underneath mechanism of circFKBP8 (also known as hsa_circ_0000915) in BC remain largely unknown.

Methods
Expression analyses were performed using quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemistry (IHC) assays. Effects on cell functional phenotypes were determined by assessing cell proliferation, migratory capacity, invasion, and stemness in vitro. The relationship between microRNA (miR)-432-5p and circFKBP8 or E2F transcription factor 7 (E2F7) was examined by RNA pull-down, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays. Xenograft assays were used to identify the function of circFKBP8 in vivo.

Results
CircFKBP8 was presented at high levels in BC tissues and cells. High circFKBP8 expression was associated with worse overall survival in BC patients. CircFKBP8 suppression inhibited BC cell proliferation, migratory capacity, invasion and stemness in vitro. CircFKBP8 suppression blocked xenograft tumor growth in vivo. Mechanistically, circFKBP8 functioned as a miR-432-5p sponge to modulate E2F7 expression. CircFKBP8 modulated BC cell malignant behaviors by miR-432-5p, and miR-432-5p affected these cell phenotypes through E2F7.

Conclusion
Our observations prove that circFKBP8 promotes BC malignant phenotypes through the miR-432-5p/E2F7 cascade, offering a promising therapeutic and prognostic target for BC.
Peritoneal-dialysis-associated peritonitis due to Staphylococcus sciuri: a case report and literature review
Authors: Ibarra-Sifuentes Héctor Raúl , Canales-Azcona Gustavo Abraham , Gómez-Arredondo Jaqueline Isabel , Rodríguez-Sifuentes Jesús Alfredo , Ríos-López Vanessa Vianey , Castellanos-Maldonado Abraham 
Published: August 2024

Abstract
Background
Peritoneal-dialysis-associated peritonitis is a serious complication of peritoneal dialysis; prevention and treatment are crucial to reduce patient morbidity and mortality. Staphylococcus sciuri is an organism that, although present in several animal species, rarely causes disease in humans, particularly in patients undergoing peritoneal dialysis.

Case presentation
A 54-year-old man with end-stage kidney disease on continuous ambulatory peritoneal dialysis was admitted to the emergency department with nausea, vomiting, slight abdominal distention, and cloudy peritoneal dialysis effluent. Relevant medical history included occupational exposure to different animal species. The white blood cell count in the peritoneal dialysis fluid sample was 11,200 cells/mm3 with 90% polymorphonuclear cells. Peritoneal-dialysis-associated peritonitis was diagnosed. Empirical antibiotic treatment was started according to the International Society for Peritoneal Dialysis guidelines. Staphylococcus sciuri was identified in the peritoneal dialysis effluent culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The treatment was modified on the basis of the sensitivity results. Medical cure was established on the basis of the negative result of the dialysate culture and absence of alterations in the cell count with differential after the end of treatment. The patient was instructed to be careful when administering peritoneal dialysis, especially in the care involved in being in contact with animals.

Conclusions
This article highlights the importance of Staphylococcus sciuri as a significant pathogen due to its virulence and antibiotic resistance, especially in patients undergoing peritoneal dialysis. Prevention strategies as well as timely diagnosis and treatment compliance according to the International Society for Peritoneal Dialysis are essential for successful outcomes.
Machine learning approach for the prediction of macrosomia
Authors: Gu Xiaochen , Huang Ping , Xu Xiaohua , Zheng Zhicheng , Luo Kaiju , Xu Yujie , Jia Yizhen , Zhou Yongjin 
Published: August 2024

AbstractFetal macrosomia is associated with maternal and newborn complications due to incorrect fetal weight estimation or inappropriate choice of delivery models. The early screening and evaluation of macrosomia in the third trimester can improve delivery outcomes and reduce complications. However, traditional clinical and ultrasound examinations face difficulties in obtaining accurate fetal measurements during the third trimester of pregnancy. This study aims to develop a comprehensive predictive model for detecting macrosomia using machine learning (ML) algorithms. The accuracy of macrosomia prediction using logistic regression, k-nearest neighbors, support vector machine, random forest (RF), XGBoost, and LightGBM algorithms was explored. Each approach was trained and validated using data from 3244 pregnant women at a hospital in southern China. The information gain method was employed to identify deterministic features associated with the occurrence of macrosomia. The performance of six ML algorithms based on the recall and area under the curve evaluation metrics were compared. To develop an efficient prediction model, two sets of experiments based on ultrasound examination records within 1-7 days and 8-14 days prior to delivery were conducted. The ensemble model, comprising the RF, XGBoost, and LightGBM algorithms, showed encouraging results. For each experimental group, the proposed ensemble model outperformed other ML approaches and the traditional Hadlock formula. The experimental results indicate that, with the most risk-relevant features, the ML algorithms presented in this study can predict macrosomia and assist obstetricians in selecting more appropriate delivery models.
Intestinal ultrasound for follow-up after 24 weeks of biological therapy in inflammatory bowel disease patients: an Egyptian center experience during the COVID-19 pandemic
Authors: El-Nakeep Sarah , Nashaat Ehab , Alsherif Fatma , Salama Mohamed Magdy 
Published: August 2024

Abstract
Background
Inflammatory bowel disease is a chronic inflammatory condition of the gut. It has two major subtypes Crohn’s disease and ulcerative colitis. The follow-up consists of radiologic, molecular, endoscopic, and histological assessments. Intestinal ultrasound (IUS) is a noninvasive measure that provides future hope in guiding the management of IBD patients. This study is to assess the effectiveness of IUS in IBD patients’ follow-up in our tertiary center during the pandemic. This is a prospective observational study during the COVID-19 pandemic. We used IUS to assess activity of IBD at baseline and at 6-month follow-up of patients on maintenance biological therapy using the following parameters: bowel haustrations, stratification, bowel wall thickness (BWT), Doppler sign (Limberg classification), presence of lymph nodes, or fibrofatty infiltration, echogenicity of the bowel, and presence of fistulae or abscesses. We compared the IUS with other radiologic parameters, histologic, and endoscopic scores at baseline before therapy, while we compared IUS with clinical scores and laboratory parameters before and after 24 weeks of biological treatment.

Results
The cohort included 50 known IBD patients from June 2021 to January 2022. The laboratory studies showed a significant improvement in the hemoglobin indices, CRP, and fecal calprotectin from baseline and after 24 weeks. BWT, lumen diameter, lymph node presence, inflammatory signs, and Doppler activity signs were the most significant parameters in detecting improvement. However, there was no significant correlation between fecal calprotectin levels and ultrasound parameters. There was a positive correlation between MR and CT enterography, endoscopic parameters, and IUS parameters at baseline.

Conclusions
IUS is an effective tool for follow-up of IBD patients especially during the pandemic periods.
The blue economy effects on EUROMED tourism: forecasting approach
Authors: Yasser Mai Mohamed , Halim Yasser Tawfik , Elmegaly Alaa A. Abd 
Published: August 2024

AbstractThis study examines the factors that influence the blue economy in EUROMED, aiming at promoting economic growth in line with the UN Sustainable Development Goals. EUROMED was chosen because all of its nations have fisheries and marine tourism, which are the two key indicators of the blue economy. Blue economy contributes to sustainable development in the fisheries and marine tourism sectors. A commitment to sustainability has sped up national and regional blue economy policy development. The study uses secondary qualitative data and literature review to analyse the synergies and conflicts between EUROMED blue economy strategies and the UN Sustainable Development Goals. The findings suggest that GDP growth, aqua production, open trade, CO2 emissions, and inflation rate influence the blue economy, and that ecotourism that considers education, society, and the environment may generate sustainable fisheries and marine tourism. Only 21 countries gave the ARDL test between 2000 and 2019. This study analyses synergies and conflicts between EUROMED blue economy strategies and UN Sustainable Development Goals. Location-based contextual development of blue economies that suit all players' requirements is essential to maintain sustainability objectives. Ensure resilience against future environmental and political shocks, preserve the ecological underpinning for vibrant blue economies, and create capacity at all levels to promote effective and fair governance. This study tries to make good use of ocean conservation and aquaculture within the context of the blue economy. This is the first EUROMED blue economy study and contributes to the theoretical and methodological development of blue economy research.