bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pfadabhängigkeit in der Mehrebenensteuerung: Das Beispiel Palliative Care
Authors: Moser Ladina , Sager Fritz 
Published: June 2015

AbstractWir argumentieren, dass das Pfadabhängigkeitskonzept des historischen Institutionalismus ein geeigneter Ansatz zur Integration des Top‐down‐ und des Bottom‐up‐Ansatzes der Mehrebenenvollzugsforschung darstellt. Unser Testfall ist die Umsetzung der Nationalen Strategie Palliative Care in den Kantonen. Mittels deskriptiver quantitativer Nachzeichnung und dreier Fallstudien wird die Pfadabhängigkeitshypothese untersucht, dass sich die Palliativversorgung in den Kantonen entlang von Pfaden entwickelt, die durch spezialisierte stationäre Einrichtungen vorgegeben werden, und dass es dem Bund kaum gelingt, diese anhand der Nationalen Strategie Palliative Care zu durchbrechen. Die Empirie bestätigt die Annahme des historischen Institutionalismus, dass positive Rückkopplungen zu Pfadabhängigkeiten führen und dadurch eine Anpassung der Institutionen an die sich wandelnde Umwelt erschwert wird, wobei sich in den Fallstudien unterschiedliche Formen positiver Rückkoppelung mit unterschiedlichen Wirkmechanismen äussern. Spezialisierte stationäre Einrichtungen können als jene Institutionen gewertet werden, die die Entwicklungspfade der Palliative Care und somit das Versorgungsarrangement in den Kantonen prägen. In Bezug auf den Einfluss des Bundes hingegen lässt sich kein allgemeingültiges Fazit ziehen. Vielmehr zeigen die Fallstudien die unterschiedliche Bedeutung kantonaler Pfade, die durch die drei Formen positiver Rückkoppelung theoretisiert werden können.
Coacervate delivery of HB‐EGF accelerates healing of type 2 diabetic wounds
Authors: Johnson Noah R. , Wang Yadong 
Published: June 2015

ABSTRACTChronic wounds such as diabetic ulcers pose a significant challenge as a number of underlying deficiencies prevent natural healing. In pursuit of a regenerative wound therapy, we developed a heparin‐based coacervate delivery system that provides controlled release of heparin‐binding epidermal growth factor (EGF)‐like growth factor (HB‐EGF) within the wound bed. In this study, we used a polygenic type 2 diabetic mouse model to evaluate the capacity of HB‐EGF coacervate to overcome the deficiencies of diabetic wound healing. In full‐thickness excisional wounds on NONcNZO10 diabetic mice, HB‐EGF coacervate enhanced the proliferation and migration of epidermal keratinocytes, leading to accelerated epithelialization. Furthermore, increased collagen deposition within the wound bed led to faster wound contraction and greater wound vascularization. Additionally, in vitro assays demonstrated that HB‐EGF released from the coacervate successfully increased migration of diabetic human keratinocytes. The multifunctional role of HB‐EGF in the healing process and its enhanced efficacy when delivered by the coacervate make it a promising therapy for diabetic wounds.
IL‐4 and IL‐13 induce protection from complement and melittin in endothelial cells despite initial loss of cytoplasmic proteins: membrane resealing impairs quantifying cytotoxicity with the lactate dehydrogenase permeability assay
Authors: Benson Barbara A. , Vercellotti Gregory M. , Dalmasso Agustin P. 
Published: June 2015

AbstractEndothelial cell activation and injury by the terminal pathway of complement is important in various pathobiological processes, including xenograft rejection. Protection against injury by human complement can be induced in porcine endothelial cells (ECs) with IL‐4 and IL‐13 through metabolic activation. However, despite this resistance, the complement‐treated ECs were found to lose membrane permeability control assessed with the small molecule calcein. Therefore, to define the apparent discrepancy of permeability changes vis‐à‐vis the protection from killing, we now investigated whether IL‐4 and IL‐13 influence the release of the large cytoplasmic protein lactate dehydrogenase (LDH) in ECs incubated with complement or the pore‐forming protein melittin. Primary cultures of ECs were pre‐treated with IL‐4 or IL‐13 and then incubated with human serum as source of antibody and complement or melittin. Cell death was assessed using neutral red. Membrane permeability was quantitated measuring LDH release. We found that IL‐4‐/IL‐13‐induced protection of ECs from killing by complement or melittin despite loss of LDH in amounts similar to control ECs. However, the cytokine‐treated ECs that were protected from killing rapidly regained effective control of membrane permeability. Moreover, the viability of the protected ECs was maintained for at least 2 days. We conclude that the protection induced by IL‐4/IL‐13 in ECs against lethal attack by complement or melittin is effective and durable despite severe initial impairment of membrane permeability. The metabolic changes responsible for protection allow the cells to repair the membrane injury caused by complement or melittin.
The Daily Resistive Index measurement useful tool in the estimation of the optimal time interval between two Shock Wave Lithotripsy sessions.
Authors: Yencilek Esin , Sarsılmaz Aysegul , Kilickesmez Ozgur , Koyuncu Hakan , Eryildirim Bilal , Gurses Bengi , Bastug Yavuz , Erihan Bilger 
Published: June 2015

Aims: To monitor the impact of Shock Wave Lithotripsy (SWL) on the renal resisive index (RI) and to investigate the potential of the RI measurement for the estimation of the optimal duration between 2 SWL sessions. Material and methods: Thirty patients with single pelvis renalis stone were included. Participitants were grouped according to their age as group 1 (<40 years, mean age 36.2±3.9 years) and group 2 (≥40 years, mean age 55.4±6.5 years). RI measurement was performed in of all patients prior to SWL. After SWL, RI was monitored daily until RI returned to their pre-SWL values. Results: The mean stone size was 2 8.97±3.62 in group 1 and 10.08±4.67 mm in group 2 (p=0.077). Following SWL, the RI value of both goups increased and the higher RI value was measured at the 24th hour as compared with their pre-SWL values (p<0.001). In day 2 RI of the groups declined, but the differences were still statistically different from their pre-SWL RI values (p<0.001). However, on the third day, RI of group 1 was close to their pre-SWL level (p=0.143). But, in group 2, RI value returned to their pre-SWL limits on day 4 (p=0.229). Conclusions: RI measurement gives important data regarding SWL related acute renal trauma and should be used as an US marker for recovery after SWL.
Where is Childhood? In Conversation With Messner and Musto
Authors: Laurendeau Jason , Konecny Dan 
Published: June 2015

In this essay, we build upon Messner and Musto’s recent call for sociologists of sport to take “kids” more seriously; we highlight that in addition to taking kids and kids’ sport more seriously, sport scholars might go further toward considering childhood not simply as a stage of life, but as a set of ideas that shape and are shaped by sporting and recreational practices and discourses. To illustrate the value of this approach, we explore a number of complexities and contradictions of contemporary risk discourses, and the ways in which these are connected to the (re)production of young people as vulnerable subjects.Dans cet essai, nous nous appuyons sur Messner et Musto qui ont récemment encouragé les sociologues du sport à prendre les enfants plus au sérieux; nous soulignons qu’en plus de prendre les enfants et les activités sportives des enfants au sérieux, les chercheurs en sport peuvent aller plus loin et considérer l’enfance non seulement comme une étape de la vie, mais aussi comme un ensemble d’idées qui forment les pratiques et discours sportifs et récréatifs et sont formées par ceux-ci. Pour illustrer le bien-fondé de cette approche, nous explorons un certain nombre de complexités et contradictions qui existent dans les discours actuels sur le risque, et les façons dont ils sont connectés à la (re)production des jeunes comme sujets vulnérables.
Gallium Compounds Exhibit Potential as New Therapeutic Agents against Mycobacterium abscessus
Authors: Abdalla Maher Y. , Switzer Barbara L. , Goss Christopher H. , Aitken Moira L. , Singh Pradeep K. , Britigan Bradley E. 
Published: June 2015

ABSTRACTThe rapidly growing nontuberculous mycobacterial speciesMycobacterium abscessushas recently emerged as an important pathogen in patients with cystic fibrosis (CF). Treatment options are limited because of the organism's innate resistance to standard antituberculous antibiotics, as well as other currently available antibiotics. New antibiotic approaches to the treatment ofM. abscessusare urgently needed. The goal of the present study was to assess the growth-inhibitory activity of different Ga compounds against an American Type Culture Collection (ATCC) strain and clinical isolates ofM. abscessusobtained from CF and other patients. In our results, using Ga(NO3)3and all of the other Ga compounds tested inhibited the growth of ATCC 19977 and clinical isolates ofM. abscessus. Inhibition was mediated by disrupting iron uptake, as the addition of exogenous iron (Fe) restored basal growth. There were modest differences in inhibition among the isolates for the same Ga chelates, and for most Ga chelates there was only a slight difference in potency from Ga(NO3)3. In contrast, Ga-protoporphyrin completely and significantly inhibited the ATCC strain and clinical isolates ofM. abscessusat much lower concentrations than Ga(NO3)3. Inin vitrobroth culture, Ga-protoporphyrin was more potent than Ga(NO3)3. WhenM. abscessusgrowth inside the human macrophage THP-1 cell line was assessed, Ga-protoporphyrin was >20 times more active than Ga(NO3)3. The present work suggests that Ga exhibits potent growth-inhibitory capacity against the ATCC strain, as well as against antibiotic-resistant clinical isolates ofM. abscessus, including the highly antibiotic-resistant strain MC2638. Ga-based therapy offers the potential for further development as a novel therapy againstM. abscessus.
Disulfiram and Copper Ions Kill Mycobacterium tuberculosis in a Synergistic Manner
Authors: Dalecki Alex G. , Haeili Mehri , Shah Santosh , Speer Alexander , Niederweis Michael , Kutsch Olaf , Wolschendorf Frank 
Published: June 2015

ABSTRACT

Tuberculosis is a severe disease affecting millions worldwide. Unfortunately, treatment strategies are hampered both by the prohibitively long treatment regimen and the rise of drug-resistant strains. Significant effort has been expended in the search for new treatments, but few options have successfully emerged, and new treatment modalities are desperately needed. Recently, there has been growing interest in the synergistic antibacterial effects of copper ions (Cu
II/I
) in combination with certain small molecular compounds, and we have previously reported development of a drug screening strategy to harness the intrinsic bactericidal properties of Cu
II/I
. Here, we describe the copper-dependent antimycobacterial properties of disulfiram, an FDA-approved and well-tolerated sobriety aid. Disulfiram was inhibitory to mycobacteria only in the presence of Cu
II/I
and exerted its bactericidal activity well below the active concentration of Cu
II/I
or disulfiram alone. No other physiologically relevant bivalent transition metals (e.g., Fe
II
, Ni
II
, Mn
II
, and Co
II
) exhibited this effect. We demonstrate that the movement of the disulfiram-copper complex across the cell envelope is porin independent and can inhibit intracellular protein functions. Additionally, the complex is able to synergistically induce intracellular copper stress responses significantly more than Cu
II/I
alone. Our data suggest that by complexing with disulfiram, Cu
II/I
is likely allowed unfettered access to vulnerable intracellular components, bypassing the normally sufficient copper homeostatic machinery. Overall, the synergistic antibacterial activity of Cu
II/I
and disulfiram reveals the susceptibility of the copper homeostasis system of
Mycobacterium tuberculosis
to chemical attacks and establishes compounds that act in concert with copper as a new class of bacterial inhibitors.
TXA709, an FtsZ-Targeting Benzamide Prodrug with Improved Pharmacokinetics and Enhanced
In Vivo
Efficacy against Methicillin-Resistant Staphylococcus aureus
Authors: Kaul Malvika , Mark Lilly , Zhang Yongzheng , Parhi Ajit K. , Lyu Yi Lisa , Pawlak Joan , Saravolatz Stephanie , Saravolatz Louis D. , Weinstein Melvin P. , LaVoie Edmond J. , Pilch Daniel S. 
Published: June 2015

ABSTRACT

The clinical development of FtsZ-targeting benzamide compounds like PC190723 has been limited by poor drug-like and pharmacokinetic properties. Development of prodrugs of PC190723 (e.g., TXY541) resulted in enhanced pharmaceutical properties, which, in turn, led to improved intravenous efficacy as well as the first demonstration of oral efficacy
in vivo
against both methicillin-sensitive
Staphylococcus aureus
(MSSA) and methicillin-resistant
S. aureus
(MRSA). Despite being efficacious
in vivo
, TXY541 still suffered from suboptimal pharmacokinetics and the requirement of high efficacious doses. We describe here the design of a new prodrug (TXA709) in which the Cl group on the pyridyl ring has been replaced with a CF
3
functionality that is resistant to metabolic attack. As a result of this enhanced metabolic stability, the product of the TXA709 prodrug (TXA707) is associated with improved pharmacokinetic properties (a 6.5-fold-longer half-life and a 3-fold-greater oral bioavailability) and superior
in vivo
antistaphylococcal efficacy relative to PC190723. We validate FtsZ as the antibacterial target of TXA707 and demonstrate that the compound retains potent bactericidal activity against
S. aureus
strains resistant to the current standard-of-care drugs vancomycin, daptomycin, and linezolid. These collective properties, coupled with minimal observed toxicity to mammalian cells, establish the prodrug TXA709 as an antistaphylococcal agent worthy of clinical development.
Activity of Meropenem Combined with RPX7009, a Novel β-Lactamase Inhibitor, against Gram-Negative Clinical Isolates in New York City
Authors: Lapuebla Amabel , Abdallah Marie , Olafisoye Olawole , Cortes Christopher , Urban Carl , Quale John , Landman David 
Published: June 2015

ABSTRACT

Multidrug-resistant
Klebsiella pneumoniae
carbapenemase (KPC)-producing
Enterobacteriaceae
are endemic to hospitals in New York City and other regions. RPX7009 is a novel β-lactamase inhibitor with activity against serine carbapenemases. We tested the activity of meropenem plus RPX7009 against 4,500 recent Gram-negative clinical isolates from 11 New York City hospitals. The meropenem-RPX7009 combination was found to have excellent
in vitro
activity against
Escherichia coli
,
K. pneumoniae
, and
Enterobacter
spp., including multidrug-resistant (MDR) KPC-producing strains. Overall, 131/133 (98.5%) KPC-producing
Enterobacteriaceae
strains were inhibited by meropenem (≤1 μg/ml) plus RPX7009 (8 μg/ml). In a limited number of strains, the combination appeared to have reduced activity against KPC-producing
K. pneumoniae
isolates with diminished
ompK35
and
ompK36
expression. The addition of RPX7009 did not affect the activity of meropenem against
Acinetobacter baumannii
and
Pseudomonas aeruginosa
. The meropenem-RPX7009 combination shows promise as a novel agent against KPC-producing
Enterobacteriaceae
and deserves further study. Other approaches will be needed to address multidrug-resistant
A. baumannii
and
P. aeruginosa
, which typically possess different mechanisms of carbapenem resistance.
Characterization of pKP-M1144, a Novel ColE1-Like Plasmid Encoding IMP-8, GES-5, and BEL-1 β-Lactamases, from a Klebsiella pneumoniae Sequence Type 252 Isolate
Authors: Papagiannitsis Costas C. , Dolejska Monika , Izdebski Radoslaw , Dobiasova Hana , Studentova Vendula , Esteves Francisco J. , Derde Lennie P. G. , Bonten Marc J. M. , Hrabák Jaroslav , Gniadkowski Marek 
Published: June 2015

ABSTRACT

IMP-8 metallo-β-lactamase was identified in
Klebsiella pneumoniae
sequence type 252 (ST252), isolated in a Portuguese hospital in 2009.
bla
IMP-8
was the first gene cassette of a novel class 3 integron, In1144, also carrying the
bla
GES-5
,
bla
BEL-1
, and
aacA4
cassettes. In1144 was located on a ColE1-like plasmid, pKP-M1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other RNA-priming plasmids, such as pJHCMW1. In1144 and pKP-M1144 represent an interesting case of evolution of resistance determinants in Gram-negative bacteria.
Draft Genome Sequence of a Strain of Cosmopolitan Fungus
Trichoderma atroviride
Authors: Shi-Kunne Xiaoqian , Seidl Michael F. , Faino Luigi , Thomma Bart P. H. J. 
Published: June 2015

ABSTRACT

An unknown fungus has been isolated as a contaminant of
in vitro
-grown fungal cultures. In an attempt to identify the contamination, we isolated the causal agent and performed whole-genome sequencing. BLAST analysis of the internal transcribed spacer (ITS) sequence against the NCBI database showed 100% identity to
Trichoderma atroviride
, and further alignment of the genome assembly confirmed the unknown fungus to be
T. atroviride
. Here, we report the draft genome sequence of a
T. atroviride
strain.
Draft Genome Sequence of
Pseudomonas
sp. Strain 10-1B, a Polycyclic Aromatic Hydrocarbon Degrader in Contaminated Soil
Authors: Bello-Akinosho Maryam , Adeleke Rasheed , Swanevelder Dirk , Thantsha Mapitsi 
Published: June 2015

ABSTRACT

Pseudomonas
sp. strain 10-1B was isolated from artificially polluted soil after selective enrichment. Its draft genome consists of several predicted genes that are involved in the hydroxylation of the aromatic ring, which is the rate-limiting step in the biodegradation of polycyclic aromatic hydrocarbons.
Draft Genome Sequence of Arthrobacter chlorophenolicus Strain Mor30.16, Isolated from the Bean Rhizosphere
Authors: Miranda-Ríos José Antonio , Ramírez-Trujillo José Augusto , Nova-Franco Bárbara , Lozano-Aguirre Beltrán Luis Fernando , Iturriaga Gabriel , Suárez-Rodríguez Ramón 
Published: June 2015

ABSTRACT

Bacteria of the genus
Arthrobacter
are commonly found in the soil and plant rhizosphere
.
In this study we report the draft genome of
Arthrobacter chlorophenolicus
strain Mor30.16 that was isolated from rhizosphere of beans grown in Cuernavaca Morelos, Mexico. This strain promotes growth and ameliorates drought stress in bean plants.
Complete Genome Sequence of Canine Papillomavirus Type 16
Authors: Luff Jennifer , Mader Michelle , Britton Monica , Fass Joseph , Rowland Peter , Orr Carolyn , Schlegel Richard , Yuan Hang 
Published: June 2015

ABSTRACT

Papillomaviruses are epitheliotropic, circular, double-stranded DNA viruses within the family
Papillomaviridae
that are associated with benign and malignant tumors in humans and animals. We report the complete genome sequence of canine papillomavirus type 16 identified within multiple pigmented cutaneous plaques and squamous cell carcinoma from an intact female Basenji dog.
Insights on Quorum-Quenching Properties of Lysinibacillus fusiformis Strain RB21, a Malaysian Municipal Solid-Waste Landfill Soil Isolate, via Complete Genome Sequence Analysis
Authors: Yong Delicia , Ee Robson , Lim Yan-Lue , Chang Chien-Yi , Yin Wai-Fong , Chan Kok-Gan 
Published: June 2015

ABSTRACT

Lysinibacillus fusiformis
strain RB21 is a quorum-quenching bacterium that is able to degrade quorum-sensing signaling molecules. Here, we present the first complete genome sequence of
L. fusiformis
strain RB21
.
The finished genome is 4.8 Mbp in size, and the quorum-quenching gene was identified.
First Draft Genome Sequence of a Human Coxiella burnetii Isolate, Originating from the Largest Q Fever Outbreak Ever Reported, the Netherlands, 2007 to 2010
Authors: Hammerl J. A. , Mertens K. , Sprague L. D. , Hackert V. H. , Buijs J. , Hoebe C. J. , Henning K. , Neubauer H. , Al Dahouk S. 
Published: June 2015

ABSTRACT

In 2009,
Coxiella burnetii
caused a large regional outbreak of Q fever in South Limburg, the Netherlands. Here, we announce the genome draft sequence of a human
C. burnetii
isolate, strain NL-Limburg, originating from this outbreak, including a brief summary of the genome's general features.
Complete Genome Sequence of an Enterotoxigenic Bacteroides fragilis Clinical Isolate
Authors: Nikitina Anastasia S. , Kharlampieva Daria D. , Babenko Vladislav V. , Shirokov Dmitriy A. , Vakhitova Maria T. , Manolov Aleksandr I. , Shkoporov Andrei N. , Taraskina Anastasia E. , Manuvera Valentin A. , Lazarev Vassili N. , Kostryukova Elena S. 
Published: June 2015

ABSTRACT

Here we present the complete genome sequence of
Bacteroides fragilis
isolate BOB25. It is an enterotoxigenic isolate that was obtained from a stool sample of a patient with dysbiosis.
Sensing of Interleukin-1 Cytokines during Streptococcus pneumoniae Colonization Contributes to Macrophage Recruitment and Bacterial Clearance
Authors: Lemon Jamie K. , Miller Megan R. , Weiser Jeffrey N. 
Published: June 2015

ABSTRACTStreptococcus pneumoniae(the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that access the cytosolic compartment via the pneumococcal pore-forming toxin pneumolysin.In vitro, cytosolic access results in cell death that includes release of the proinflammatory cytokine interleukin-1β (IL-1β). IL-1 family cytokines, including IL-1β, are secreted upon activation of inflammasomes, although the role of this activation in the host immune response to pneumococcal carriage is unknown. Using a murine model of pneumococcal nasopharyngeal colonization, we show that mice deficient in the interleukin-1 receptor type 1 (Il1r1−/−) have reduced numbers of neutrophils early after infection, fewer macrophages later in carriage, and prolonged bacterial colonization. Moreover, intranasal administration of Il-1β promoted clearance. Macrophages are the effectors of clearance, and characterization of macrophage chemokines in colonized mice revealed thatIl1r1−/−mice have lower expression of the C-C motif chemokine ligand 6 (CCL6), correlating with reduced macrophage recruitment to the nasopharynx. IL-1 family cytokines are known to promote adaptive immunity; however, we observed no difference in the development of humoral or cellular immunity to pneumococcal colonization between wild-type andIl1r1−/−mice. Our findings show that sensing of IL-1 cytokines during colonization promotes inflammation without immunity, which may ultimately benefit the pneumococcus.