bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human reproduction comes at the expense of faster aging and a shorter life
Authors: Hukkanen Mikaela , Kankaanpää Anna , Heikkinen Aino , Kaprio Jaakko , Cristofari Robin , Ollikainen Miina 
Published: July 2024

ABSTRACTEvolutionary theories suggest a trade-off between resources allocated to reproduction and those allocated to self-maintenance, and predict that higher reproductive output entails a shorter lifespan. This study investigates the impact of childbearing on aging and lifespan using data from contemporary Finnish twin women. We model the association between reproductive trajectories and survival in 17,080 women, and assess biological aging in a subset of participants (N=1,117) using the PCGrimAge clock, an algorithm trained to predict biological aging and mortality risk from DNA methylation. Our findings suggest that early childbearing, numerous pregnancies or nulliparity all contribute to accelerated aging and increased mortality risk. These results provide strong evidence for the existence of a trade-off between reproduction, aging and lifespan in modern humans, and provide novel insights into the genetic and lifestyle determinants of healthspan.
Structural basis for the interaction between the bacterial cell division proteins FtsZ and ZapA
Authors: Fujita Junso , Hibino Kota , Kagoshima Gota , Kamimura Natsuki , Kato Yuki , Uehara Ryo , Namba Keiichi , Uchihashi Takayuki , Matsumura Hiroyoshi 
Published: July 2024

AbstractCell division in most bacteria is regulated by the tubulin homolog FtsZ as well as ZapA, a FtsZ-associated protein. However, how FtsZ and ZapA function coordinately has remained elusive. Here we report the cryo-electron microscopy structure of the ZapA-FtsZ complex. The complex forms an asymmetric ladder-like structure, in which the double antiparallel FtsZ protofilament on one side and a single protofilament on the other side are tethered by ZapA tetramers. In the complex, the extensive interactions of FtsZ with ZapA cause a structural change of the FtsZ protofilament, and the formation of the double FtsZ protofilament increases electrostatic repulsion. High-speed atomic force microscopy analysis revealed cooperative interactions of ZapA with FtsZ at a molecular level. Our findings not only provide a structural basis for the interaction between FtsZ and ZapA but also shed light on how ZapA binds to FtsZ protofilaments without disturbing FtsZ dynamics to promote cell division.
FLOW CYTOMETRIC PROCEDURES FOR DEEP CHARACTERIZATION OF NANOPARTICLES
Authors: Tirelli Valentina , Grasso Felicia , Barreca Valeria , Polignano Deborah , Gallinaro Alessandra , Cara Andrea , Sargiacomo Massimo , Fiani Maria Luisa , Sanchez Massimo 
Published: July 2024

AbstractIn recent years, there has been a notable increasing interest surrounding the identification and quantification of nanosized particles, including extracellular vesicles (EVs) and viruses. The challenge posed by the nano-sized dimension of these particles makes precise examination a significant undertaking.Among the different techniques for the accurate study of EVs, Flow cytometry (FCM) stands out as the ideal method. It is characterized by high sensitivity, low time consumption, non-destructive sampling, and high throughput.In this article, we propose the optimization of FCM procedures to identify, quantify, and purify EVs and virus like particles (VLPs).The protocol aims to reduce artifacts and errors in nano-sized particles counting, overall caused by the swarming effect. Different threshold strategies were compared to ensure result specificity. Additionally, the critical parameters to consider when using conventional FCM outside of the common experimental context of use have also been identified. Finally, fluorescent-EVs sorting protocol was also developed with highly reliable results using a conventional cell sorter.
An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization
Authors: Ma Hongqiang , Chen Maomao , Xu Jianquan , Zhao Yongxin , Liu Yang 
Published: July 2024

AbstractThe mesoscope has emerged as a powerful imaging tool in biomedical research, yet its high cost and low resolution have limited its broader application. Here, we introduce the Omni-Mesoscope, a cost-effective high-spatial-temporal, multimodal, and multiplex mesoscopic imaging platform built from cost-efficient off-the-shelf components. This system uniquely merges the capabilities of quantitative phase microscopy to capture live-cell dynamics over a large cell population with highly multiplexed fluorescence imaging for comprehensive molecular characterization. This integration facilitates simultaneous tracking of live-cell morphodynamics across thousands of cells, alongside high-content molecular analysis at the single-cell level. Furthermore, the Omni-Mesoscope offers a mesoscale field of view of approximately 5 mm2with a high spatial resolution down to 700 nm, enabling the capture of information-rich images with detailed sub-cellular features. We demonstrate such capability in delineating molecular characteristics underlying rare dynamic cellular phenomena, such as cancer cell responses to chemotherapy and the emergence of polyploidy in drug-resistant cells. Moreover, the cost-effectiveness and the simplicity of our Omni-Mesoscope democratizes mesoscopic imaging, making it accessible across diverse biomedical research fields. To further demonstrate its versatility, we integrate expansion microscopy to enhance 3D volumetric super-resolution imaging of thicker tissues, opening new avenues for biological exploration at unprecedented scales and resolutions.
Systematic Analysis of Human Colorectal Cancer scRNA-seq Revealed Limited Pro-tumoral IL-17 Production Potential in Gamma Delta T Cells
Authors: Ran Ran , Trapecar Martin , Brubaker Douglas K. 
Published: July 2024

AbstractGamma delta (γδ) T cells play a crucial role in anti-tumor immunity due to their cytotoxic properties. However, the role and extent of γδ T cells in production of pro-tumorigenic interleukin-17 (IL-17) within the tumor microenvironment (TME) of colorectal cancer (CRC) remains controversial. In this study, we re-analyzed nine published human CRC whole-tissue single-cell RNA sequencing (scRNA-seq) datasets, identifying 18,483 γδ T cells out of 951,785 total cells, in the neoplastic or adjacent normal tissue of 165 human CRC patients. Our results confirm that tumor-infiltrating γδ T cells exhibit high cytotoxicity-related transcription in both tumor and adjacent normal tissues, but critically, none of the γδ T cell clusters showed IL-17 production potential. We also identified various γδ T cell subsets, including Teff, TRM, Tpex, and Tex, and noted an increased expression of cytotoxic molecules in tumor-infiltrating γδ T cells compared to their normal area counterparts. Our work demonstrates that γδ T cells in CRC primarily function as cytotoxic effector cells rather than IL-17 producers, mitigating the concerns about their potential pro-tumorigenic roles in CRC, highlighting the importance of accurately characterizing these cells for cancer immunotherapy research and the unneglectable cross-species discrepancy between the mouse and human immune system in the study of cancer immunology.
Auditory-motor entrainment and listening experience shape the perceptual learning of concurrent speech
Authors: MacLean Jessica , Stirn Jack , Bidelman Gavin M. 
Published: July 2024

ABSTRACTBackgroundPlasticity from auditory experience shapes the brain’s encoding and perception of sound. Though prior research demonstrates that neural entrainment (i.e., brain-to-acoustic synchronization) aids speech perception, how long- and short-term plasticity influence entrainment to concurrent speech has not been investigated. Here, we explored neural entrainment mechanisms and the interplay between short- and long-term neuroplasticity for rapid auditory perceptual learning of concurrent speech sounds in young, normal-hearing musicians and nonmusicians.MethodParticipants learned to identify double-vowel mixtures during ∼45 min training sessions with concurrent high-density EEG recordings. We examined the degree to which brain responses entrained to the speech-stimulus train (∼9 Hz) to investigate whether entrainment to speech prior to behavioral decision predicted task performance. Source and directed functional connectivity analyses of the EEG probed whether behavior was driven by group differences auditory-motor coupling.ResultsBoth musicians and nonmusicians showed rapid perceptual learning in accuracy with training. Interestingly, listeners’ neural entrainment strength prior to target speech mixtures predicted behavioral identification performance; stronger neural synchronization was observed preceding incorrect compared to correct trial responses. We also found stark hemispheric biases in auditory-motor coupling during speech entrainment, with greater auditory-motor connectivity in the right compared to left hemisphere for musicians (R>L) but not in nonmusicians (R=L).ConclusionsOur findings confirm stronger neuroacoustic synchronization and auditory-motor coupling during speech processing in musicians. Stronger neural entrainment to rapid stimulus trains preceding incorrect behavioral responses supports the notion that alpha-band (∼10 Hz) arousal/suppression in brain activity is an important modulator of trial-by-trial success in perceptual processing.
Cerebrospinal Fluid and Plasma Metabolites with Parkinson’s Disease: A Mendelian Randomization Study
Authors: Wang Jia-Li , Zheng Ran , Fang Yi , Cao Jin , Zhang Bao-Rong 
Published: July 2024

AbstractBackground and ObjectivePrevious studies have identified associations between metabolites and Parkinson’s disease (PD), but the causal relationships remain unclear. This study aims to identify causal relationships between specific cerebrospinal fluid (CSF) and plasma metabolites and the PD risk using Mendelian Randomization (MR).MethodsWe utilized data on 338 CSF metabolites from the Wisconsin Alzheimer’s Disease Research Center and the Wisconsin Registry for Alzheimer’s Prevention, and 1,400 plasma metabolites from the Canadian Longitudinal Study on Aging. PD outcome data were obtained from a GWAS meta-analysis by the International Parkinson’s Disease Genomics Consortium. MR analysis was conducted using the TwoSampleMR package in R.ResultsMR analysis identified 49 plasma metabolites with suggestive causal relationships with PD risk, including 21 positively associated metabolites, 23 negatively associated metabolites, and 5 unknown compounds. In the CSF, six metabolites showed suggestive causal relationships with PD, including positively associated dimethylglycine, gluconate, oxalate (ethanedioate), and the unknown metabolite X-12015, while (1-enyl-palmitoyl)-2-arachidonoyl-GPC (P-16:0/20:4) and the unknown metabolite X-23587 were negatively associated. Among the plasma metabolites, those with a positive association with PD risk include hydroxy-3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (hydroxy-CMPF), carnitine C14, 1-linoleoyl-GPG (18:2), glucose to maltose ratio, and cis-3,4-methyleneheptanoate. Conversely, metabolites with a negative association with PD risk include tryptophan, succinate to acetoacetate ratio, N,N,N-trimethyl-alanylproline betaine (TMAP), glucuronide of piperine metabolite C17H21NO3, and linoleoylcholine.ConclusionOur study underscores the correlation between CSF and plasma metabolites and PD risk, highlighting specific metabolites as potential biomarkers for diagnosis and therapeutic targets.
Eyes on VR: Unpacking the Causal Chain Between Exposure, Reception, and Retention for Emotional Billboard Messages
Authors: Cho Hee Jung , Lim Sue , Turner Monique Mitchell , Bente Gary , Schmälzle Ralf 
Published: July 2024

AbstractThe causal chain from message exposure to reception to effects is widely accepted as the basic explanatory model for communication outcomes. Problematically, the chain’s links are often studied in isolation, leaving measurement gaps that compromise the ecological validity and practical utility of experimental research. Here we introduce a VR-based paradigm that encompasses a realistic message reception context, i.e., a simulated car ride on a highway flanked by billboards. We varied attentional message factors (emotional content) as well as contextual task distractions (trash-counting). VR-integrated eye trackers were used to capture participants’ incidental message exposure dependent on their actual gaze behavior. Consistent with our predictions, results show that 1. exposure gates all subsequent effects; 2. distraction impacts likelihood of exposure; 3. both the manipulation of emotional content and distraction affect retention. This comprehensive analysis of the exposure-reception-retention chain can be broadly applied to a variety of message reception contexts that will be discussed.
Does perceived overqualification lead to cyberloafing? A moderated-mediation model based on social cognitive theory
Authors: Wang Min , Liu Yuqi , Lang Yi 
Published: July 2024

Purpose
Based on social cognitive theory, this study aims to investigate the influence of perceived overqualification (POQ) on employees’ cyberloafing behavior. The mediating role of moral disengagement and the moderating roles of organizational identification (OID) and organizational decline are further examined.

Design/methodology/approach
The authors collected 740 valid questionnaires from participants across multiple organizations. To minimize common method bias (CMB) and enhance the reliability of the findings, data were gathered at two different time points, with a 30-day interval.

Findings
POQ positively impacts cyberloafing through the mechanism of moral disengagement. Additionally, the indirect relationship between POQ and cyberloafing via moral disengagement is moderated by OID and organizational decline. Specifically, a higher degree of OID weakens the indirect effect of POQ on cyberloafing, while a higher level of perceived organizational decline strengthens this effect.

Originality/value
While most existing studies on cyberloafing focus on insufficient resources, such as role conflict and workload, the authors propose that surplus personal resources, exemplified by POQ, can also lead to cyberloafing. This research contributes to a broader understanding of antecedents of cyberloafing, highlighting the mechanism of ethical considerations and the interplay between personal qualifications, organizational identification and organizational decline.
Analysis of reflection of wave propagation in magneto-thermoelastic nonlocal micropolar orthotropic medium at impedance boundary
Authors: Yadav Anand Kumar , Mahato Hari Shankar , Kumari Sangeeta , Jurczak Pawel 
Published: July 2024

Purpose
This study aims to examine the plane wave reflection problem in micropolar orthotropic magneto-thermoelastic half space, considering the influence of impedance as a boundary in a nonlocal elasticity.

Design/methodology/approach
This study presents the novel formulation of governing partial differential equations for micropolar orthotropic medium with impact of nonlocal thermo-elasticity under magnetic field.

Findings
This study provides the numerical results validation for a particular numerical data and expression for the amplitude ratios of reflected waves and identifies the existence of four different waves, namely, quasi longitudinal displacement

qCLD-wave, quasi thermal wave

qCT-wave, quasi transverse displacement

qCTD-wave and quasi-transverse micro-rotational

qCTM-wave. The study derives the velocity equation giving the speed and phase velocity of these waves. The study also shows that the small-scale size effect gives significant impact on phase velocity.

Research limitations/implications
The graphical analysis examines the variation of speeds and coefficients of attenuation of these waves due to frequency, magnetic field and nonlocal parameters. Also, significant conclusions on the variation of reflection coefficient against nonlocal parameter, frequency, impedance parameter and angle of incidence are provided graphically.

Practical implications
The creation of more effective micropolar orthotropic anisotropic materials which are very useful in the daily life and their applications in earth science are greatly impacted by the findings of this study.

Originality/value
The authors of the submitted document initiated and produced it collectively, with equal contributions from all members.
Intrapreneurship research: What have we learned and where can we go from here?
Authors: Farrukh Muhammad , Ghazzawi Issam 
Published: July 2024

Purpose
The study aims to analyze the evolution of intrapreneurship in terms of its productivity and impact while identifying major themes in the literature. It also seeks to suggest future research directions.

Design/methodology/approach
This study employs bibliometric analysis utilizing the Scopus database. The study used the number of publications and citations to measure the productivity and impact. In addition, bibliographic coupling was used to map intellectual networking. A content analysis was also performed to identify the antecedents and outcomes of intrapreneurship.

Findings
The study identifies the trends and trajectories of intrapreneurship research in terms of performance and impact. Based on the content analysis of intrapreneurship literature, the study finds outcomes and major domains of antecedents.

Originality/value
This research contributes to the evolving intrapreneurship field by comprehensively analyzing its changing landscape. It offers valuable insights and recommendations for future research, highlighting potential areas for further exploration and deepening the understanding of intrapreneurship within the organizational context.
Digitization strategy and innovation performance of microfinance institutions: mediating role of innovation capability
Authors: Abera Mekuanint , Marvadi Chetana , Suthar Dilipkumar 
Published: July 2024

Purpose
This study aims to examine the mediating role of innovation capability in the relationship between digital transformation strategy and innovation performance of microfinance institutions in Ethiopia.

Design/methodology/approach
Survey data were collected from 12 microfinance institutions in Ethiopia through self-administered questionnaires. Statistical analysis was conducted using structural equation modeling with AMOS and SPSS. Covariance-based structural equation modeling was used to test the study hypotheses.

Findings
Digital transformation strategy indicators such as (digitization vision, information technology integration, information technology agility and flexibility of information technology) directly affect innovation performance. The innovation capability mediates the relationship between digital transformation strategy indicator (information technology agility) and innovation performance. However, innovation capability does not have mediation effect in the relationship between digital transformation strategy remaining indicators (digitization vision, information technology flexibility and information technology integration) and innovation performance.

Originality/value
The study affirmed the importance of dynamic capability theory and presents noteworthy conclusions applicable to managers, stakeholders, and policymakers. It illuminates how innovation capability serves as a crucial link between digital transformation strategies and innovation performance within microfinance institutions in Ethiopia. This research enhances the current understanding of innovation capability, digital transformation strategy and innovation performance in the literature.
Attenuating workplace cynicism among non-family employees in family firms: influence of mindful leadership, belongingness and leader–member exchange quality
Authors: Razzak Mohammad Rezaur , Bachkirov Alexandre Anatolievich 
Published: July 2024

PurposeDrawing on mindfulness theory, this study attempts to gain insights into whether leader-mindfulness (LM) influences workplace cynicism (WPC) among non-family employees (NFEs) working in small and medium-sized private family firms. Furthermore, the study leverages the self-determination theory to examine if the above relationship is mediated by the belongingness of the NFEs to the organization and leader–member exchange quality (LMXQ).Design/methodology/approachA conceptual framework with a set of hypotheses is developed. Using cross-sectional survey data collected from 376 NFEs working in small and medium-sized private family firms in Oman, the hypotheses are tested using structural equation modeling that was analyzed through PLS-SEM.FindingsThe results indicate that LM influences WPC both directly and through the mediating effect of LMXQ. The findings also suggest that, while LM influences belongingness, and that belongingness also mediates the relationship between LM and LMXQ. However, belongingness does not mediate the relationship between LM and WPC, either by itself or serially.Practical implicationsThe prevalence of WPC among NFEs in family firms hinders the ability of such organizations to retain talented employees that are outside the family circle. Hence, this study presents nuanced insights to those who manage such organizations, as it reveals that leading the family business mindfully can markedly reduce WPC among NFEs, particularly when LM enhances LMXQ.Originality/valueThe study makes four novel contributions. First, this appears to be the first study at the crossroads of the family business and organizational behavior literature to investigate the under-researched topic of WPC among NFEs in family firms. Second, the study provides insights into the relationship between LM and WPC by developing a conceptual framework that draws on mindfulness theory and self-determination theory. Third, it identifies the mediating role of LMXQ in the link between LM and WPC. Finally, it reveals that, although the belongingness of NFEs to their organization is influenced by LM, it does not automatically influence WPC.
Quantifying the quantile connectedness among Memecoin, Halal ETF and ESG index
Authors: Tabassum Sabia , Rashid Umra , Rabbani Mustafa Raza , Yadav Miklesh Prasad 
Published: July 2024

Purpose
The purpose of this paper is to examine the connectedness among Memecoin, Halal exchange traded funds (ETF) and environmental, social and governance (ESG) indexes in different quantiles.

Design/methodology/approach
The authors consider Dogecoin to measure Memecoin while Wahed FTSE USA Shariah ETF (HLAL) and SP Funds S&P 500 Sharia Industry Exclusions ETF (SPUS) are used to represent Halaf ETF. Similarly, iShares ESG Aware MSCI USA ETF (ESGU) and Vanguard ESG US Stock (ESGV) proxy the ESG index ETF. The daily price of these examined markets is considered from January 2, 2020, to January 18, 2024. The quantile vector autoregression is deployed for the empirical computation.

Findings
The result reveals that Memecoin (Dogecoin) emerges as the best diversifier irrespective of various quantiles because it is least connected in terms of recipient and transmission of shock. In addition, the authors observe an intriguing observation that the total connectedness in higher quantile is large, followed by lower quantile.

Originality/value
This study is undertaken considering the novelty in the form of the proxies of examined markets along with natural outbreak (COVID-19) and man-made outbreak (Russia–Ukraine invasion) periods.