bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydrology of Blue Lake in the Dinaric karst
Authors: Bonacci Ognjen , Andrić Ivo , Yamashiki Yosuke 
Published: January 2013

AbstractThe aim of this paper is to analyse hydrological measurements obtained from Blue Lake located near the town of Imotski (Croatia), during the period from 6 November 2009 at 10:26 h to 4 September 2010 at 03:26 h (7230 h or 302 days). The water depth, water temperature and electrical conductivity of the water were continuously measured during this period. The measurements were obtained with a CTD DIVER (Schlumberger Water Services). The instrument was fixed at the bottom of Blue Lake, which means that all the measured data refer to a single point of measurement. The data represent the first systematic and continuous monitoring of the hydrological parameters of this fascinating karst phenomenon. The hydrological analysis also involved daily rainfall data and daily mean air temperatures recorded at the nearby Imotski meteorological station. The rate of water level rise and fall and the hourly and daily average inflow and outflow into and from the lake were calculated using the available data. The analysis led to the conclusion that Blue Lake is mainly recharged by water coming from the karst aquifer, the dimensions and the characteristics of which have not yet been adequately studied. Copyright © 2013 John Wiley & Sons, Ltd.
Chicana Bloggers: Creating Diversity Online Via Participation
Authors: Santoy Janie Jaramillo 
Published: January 2013

AbstractThis column addresses the importance of acknowledging the knowledge‐making practice of Chicana bloggers. The author argues the online practices of Chicanas can be used to determine ways to develop 21st‐century literacies of students, especially students of color. The author presents findings from a case study of three Chicana bloggers that reveal several critical practices involving naming and labeling conventions, visual site design, content choices, and social connections. These strategies demonstrate how these bloggers use digital technology to establish their ethnic and political identity online.
Kinetic studies of ethanol fermentation using Kluyveromyces sp. IIPE453
Authors: Kumar Sachin , Dheeran Pratibha , Singh Surendra P. , Mishra Indra M. , Adhikari Dilip K. 
Published: January 2013

AbstractBackgroundTo operate the fermentation process effectively and efficiently, the kinetic modeling of cell growth and ethanol fermentation is necessary to predict the results of industrial fermentations under the optimized conditions.ResultsA kinetic study was conducted for glucose utilization for growth of the yeast strain Kluyveromyces sp. IIPE453 and ethanol formation. Effect of temperature, pH and initial glucose concentration on cell growth and ethanol formation was studied. The data obtained experimentally were validated with existing kinetic models for product and/or substrate inhibition.ConclusionOf all the models, the Aiba model for substrate inhibition was found to be the best fit to the experimental data for the growth of Kluyveromyces sp. IIPE453, and the Luong model for product inhibition was found to be the best fit for ethanol formation using Kluyveromyces sp. IIPE453. © 2013 Society of Chemical Industry
Role of HSP70 in the regulation of the testicular apoptosis in a seasonal breeding teleost Prochilodus argenteus from the São Francisco river, Brazil
Authors: Domingos Fabricio F.T. , Thomé Ralph G. , Martinelli Patrícia M. , Sato Yoshimi , Bazzoli Nilo , Rizzo Elizete 
Published: January 2013

ABSTRACTThis study investigated the relationship among heat shock protein 70 (HSP70), proliferating cell nuclear antigen (PCNA), and testicular apoptosis during a breeding cycle of Prochilodus argenteus, a neotropical migratory characiform fish of importance in commercial fishery from the São Francisco River basin. A total of 48 (12 fish/sampling) adult males were caught using casting and drifting nets in four samplings from June 2008 to March 2009. Immunohistochemistry, Western blotting, terminal transferase‐mediated dUTP nick‐end labeling (TUNEL), enzyme‐linked immunosorbent assay (ELISA), and caspase‐3 colorimetric assay were assessed in different phases of spermatogenesis. Labeling for HSP70 occurred in spermatogonia (SPGA 18.0±1.5 and SPGB 27.9±1.0 in 100 mm2, respectively) and Sertoli cells in all sampling periods, with higher values in June (resting period) while spermatocytes were labeled in September (maturation period) and December (ripe period). For PCNA, immunoreaction was predominant in spermatogonia in June and September, while primary spermatocytes were labeled mainly in December (18.7±2.0). TUNEL‐positive reaction occurred throughout the sampling periods, and labeling was detected in the nucleus of germ cells in all developmental phases, except spermatozoa. By ELISA, total HSP70 in testis increased significantly from June to December, and decreased in March (regression period), P<0.05. Caspase‐3 activity decreased from June to December and increased in March. Taken together, our results suggest that HSP70 may protect the germ cells from caspase‐3‐dependent apoptosis during testicular activity and, reduction of HSP70 and increase of apoptosis contribute for testicular remodeling after the breeding season in wild populations of P. argenteus in the São Francisco River. Microsc. Res. Tech. 76:350–356, 2013. © 2013 Wiley Periodicals, Inc.
Marsupial morphology of reproduction: South America opossum male model
Authors: De Barros Michelle Andrade , Panattoni Martins João Flávio , Samoto Vivian Yochiko , Oliveira Vanessa Cristina , Gonçalves Natalia , Furlaneto. Mançanares Celina Almeida , Vidane Atanasio , Carvalho Ana Flávia , Ambrósio Carlos Eduardo , Miglino Maria Angélica 
Published: January 2013

ABSTRACTThis study aims to describe the morphology of Didelphis sp. male genital organs (penis, testes, epididymis, ductus deferens, prostate, and bulbourethral gland). Ten male animals were used, eight for macroscopic and light microscopy analysis, and two for scanning electron microscopy. The testes and epididymis showed similarity to other eutherian mammals. The bifid penis showed the urethra ending in the medial region where the bifurcation begins, occurring in each segment extension of the urethral groove until the beginning of the glans. Histologically, the penis consists of a cavernous and spongy body, covered by stratified squamous epithelium with loose connective tissue. The urethra was lined by transitional stratified epithelium. In the prostate, prostatic segments were found consisting of tubular glands in a radial arrangement around the urethra, coated externally by a dense connective tissue associated with a relatively thick layer of smooth muscle arranged in two layers that surround the glandular tissue. The animals had three pairs of bulbourethral glands placed at the membranous and cavernous urethra junction with descending and parallel excretory ducts ending caudally in the urethral lumen. Microsc. Res. Tech. 76:388–397, 2013. © 2013 Wiley Periodicals, Inc.
Effectiveness of HPV 16 viral load and the E2/E6 ratio for the prediction of cervical cancer risk among Chinese women
Authors: Chang Lihong , He Xiaoxiao , Yu Guifang , Wu Yuping 
Published: January 2013

AbstractThe effectiveness of the E2/E6 ratio, the state of viral genome integration and the viral load of human papillomavirus 16 (HPV 16) in predicting the risk of cervical cancer among Chinese women was investigated. Quantitative PCRs for the E2/E6 ratio and the viral load were performed on 85 cervical cancer samples and 55 HPV 16 positive healthy controls. The integrated form of the viral genome was found in 10.9% control samples and in 26.4% cervical cancer samples (P = 0.02). The majority of the cervical cancer (63.2%) and control samples (60%) were mixed forms. The E2/E6 ratio was associated with a high risk of cervical cancer (OR = 7.29, P = 9.55E−6). The integrated form (OR = 6.54, P = 0.005) and mixed form (OR = 2.93, P = 0.042) increased the risk of cervical cancer. The mean viral load in cervical cancer samples (37,371 ± 227,135) was higher than that in the controls (4,619 ± 27,079; P = 0.011). Additionally, the viral load increased along with the cervical cancer progression from the International Federation of Gynecology and Obstetrics (FIGO) stage I (12,337 ± 25,604) to stage II (67,453 ± 319,821). Compared with the state of viral genome integration (area under the receiver operating characteristic curve (AUC) = 0.743) or the viral load (AUC = 0.694), the E2/E6 ratio improved the effectiveness of the risk prediction of cervical cancer (AUC = 0.777), with the sensitivity (specificity) 81.2% (71.7%). The state of viral genome integration and the viral load of HPV 16 were important factors for the risk prediction of cervical cancer among Chinese women, and the E2/E6 ratio had a better cervical cancer risk prediction with age adjustment. J. Med. Virol. 85:646–654, 2013. © 2013 Wiley Periodicals, Inc.
Diagnostic and prognostic value of Ischemia‐modified albumin in patients with Crimean‐Congo hemorrhagic fever
Authors: Mentese Ahmet , Koksal Iftihar , Sumer Aysegül Uzun , Arslan Mustafa , Karahan S. Caner , Yılmaz Gürdal 
Published: January 2013

AbstractThe aim of this study was to determine the diagnostic and prognostic significance of ischemia modified albumin (IMA) levels in patients with Crimean‐Congo hemorrhagic fever (CCHF). This retrospective study was conducted with patients with CCHF. IMA levels in patients with CCHF were determined using the rapid colorimetric method. IMA levels of CCHF patients were significantly higher compared with the control group (P = 0.0001). At an IMA cut‐off point of 0.555 ABSU (absorbance units), sensitivity was 65.1%, specificity 82.5%, positive predictive values (PPV) 82.5%, and negative predictive values (NPV) 65.1%. IMA levels of patients with hemorrhage were significantly higher compared with patients without hemorrhage (P = 0.005). IMA has been validated as both a new and sensitive ischemia and oxidative stress biomarker. In addition to its diagnostic significance, IMA investigated in CCHF patients at time of arrival may be an important marker with its prognostic role in determining in the early stage whether the disease will follow a hemorrhagic course. J. Med. Virol. 85:684–688, 2013. © 2013 Wiley Periodicals, Inc.
An overlapping Yee finite‐difference time‐domain method for material interfaces between anisotropic dielectrics and general dispersive or perfect electric conductor media
Authors: Liu Jinjie , Brio Moysey , Moloney Jerome V. 
Published: January 2013

SUMMARYA novel stable anisotropic finite‐difference time‐domain (FDTD) algorithm based on the overlapping cells is developed for solving Maxwell's equations of electrodynamics in anisotropic media with interfaces between different types of materials, such as the interface between anisotropic dielectrics and dispersive medium or perfect electric conductor (PEC). The previous proposed conventional anisotropic FDTD methods suffer from the late‐time instability due to the extrapolation of the field components near the material interface. The proposed anisotropic overlapping Yee FDTD method is stable, as it relies on the overlapping cells to provide the collocated field values without any interpolation or extrapolation. Our method has been applied to simulate electromagnetic invisibility cloaking devices with both anisotropic dielectrics and PEC included in the computational domain. Numerical results and eigenvalue analysis confirm that the conventional anisotropic FDTD method is weakly unstable, whereas our method is stable. Copyright © 2013 John Wiley & Sons, Ltd.
CX3CL1/CX3CR1 regulates nerve injury‐induced pain hypersensitivity through the ERK5 signaling pathway
Authors: Sun Jian‐Liang , Xiao Chun , Lu Bo , Zhang Juan , Yuan Xiao‐zong , Chen Wei , Yu Li‐Na , Zhang Feng‐Jiang , Chen Gang , Yan Min 
Published: January 2013

AbstractPeripheral nerve injury induces the cleavage of CX3CL1 from the membrane of neurons, where the soluble CX3CL1 subsequently plays an important role in the transmission of nociceptive signals between neurons and microglia. Here we investigated whether CX3CL1 regulates microglia activation through the phosphorylation of extracellular signal‐regulated protein kinase 5 (ERK5) in the spinal cord of rats with spinal nerve ligation (SNL). ERK5 and microglia were activated in the spinal cord after SNL. The knockdown of ERK5 by intrathecal injection of antisense oligonucleotides suppressed the hyperalgesia and nuclear impact of nuclear factor‐κB induced by SNL. The blockage of CX3CR1, the receptor of CX3CL1, significantly reduced the level of ERK5 activation following SNL. In addition, the antisense knockdown of ERK5 reversed the CX3CL1‐induced hyperalgesia and spinal microglia activation. Our study suggests that CX3CL1/CX3CR1 regulates nerve injury‐induced pain hypersensitivity through the ERK5 signaling pathway. © 2013 Wiley Periodicals, Inc.
A novel neurofibromin (NF1) interaction with the leucine‐rich pentatricopeptide repeat motif‐containing protein links neurofibromatosis type 1 and the french canadian variant of leigh's syndrome in a common molecular complex
Authors: Arun Vedant , Wiley Joseph C. , Kaur Harpreet , Kaplan David R. , Guha Abhijit 
Published: January 2013

AbstractLoss‐of‐function mutations and deletions in the neurofibromin tumor suppressor gene (NF1) cause neurofibromatosis type 1 (NF‐1), the most common inherited syndrome of the nervous system in humans, with a birth incidence of 1:3,000. The most visible features of NF‐1 are the neoplastic manifestations caused by the loss of Ras‐GTPase‐activating protein (Ras‐GAP) activity mediated through the GAP‐related domain (GRD) of neurofibromin (NF1), the protein encoded by NF1. However, the syndrome is also characterized by cognitive dysfunction and a number of developmental abnormalities. The molecular etiology of many of these nonneoplastic phenotypes remains unknown. Here we show that the tubulin‐binding domain (TBD) of NF1 is a binding partner of the leucine‐rich pentatricopeptide repeat motif‐containing (LRPPRC) protein. These two proteins complex with Kinesin 5B, hnRNP A2, Staufen1, and Myelin Basic Protein (MBP) mRNA, likely in RNA granules. This interaction is of interest in that it links NF‐1 with Leigh's syndrome, French Canadian variant (LSFC), an autosomal recessive neurodegenerative disorder that arises from mutations in the LRPPRC gene. Our findings provide clues to how loss or mutation of NF1 and LRPPRC may contribute to the manifestations of NF‐1 and LSFC. © 2013 Wiley Periodicals, Inc.
Use of hydrodistillation and headspace solid‐phase microextraction to characterize the volatile composition of different hop cultivars
Authors: Vázquez‐Araújo Laura , Rodríguez‐Solana Raquel , Cortés‐Diéguez Sandra M , Domínguez José M. 
Published: January 2013

AbstractBackgroundHop cones, the immature inflorescences of the female plant of Humulus lupulus L., have been used for centuries to improve the flavor of beer and can be also used for a great variety of other products. Four samples of hop, belonging to three different cultivars (Nugget, Saaz and Perle), were studied in the present work. Headspace solid‐phase microextraction and hydrodistillation techniques were used to obtain the volatile profiles of the samples.ResultsIndependent of the technique employed, over 40 volatile compounds were detected in the hop pellet samples (esters, monoterpenes, monoterpenoids, sesquiterpenes and sesquiterpenoids). Sesquiterpenes and sesquiterpenoids represented the majority of the total aromatic compounds. The main compounds for all cultivars were myrcene, β‐caryophyllene and humulene, but the presence of high amounts of β‐farnesene in Saaz cultivar was highlighted.ConclusionBoth techniques were suitable for studying qualitatively the volatile composition of hop pellets, but some differences were shown when studying the proportion of the main constituents of the volatile profiles. Understanding these differences may help researchers design future studies to advise the industry how to exploit the potential of each hop cultivar. © 2013 Society of Chemical Industry
Nasal nitric oxide levels do not allow for discrimination between olfactory loss due to various etiologies
Authors: Gupta Neelima , Drusch Jenny , Landis Basile N. , Hummel Thomas 
Published: January 2013

AbstractObjectives/Hypothesis:Nasal nitric oxide (NO) and olfactory function are decreased in patients with chronic inflammatory sinonasal disease, suggesting a link between these two parameters. The aim of the study was to investigate nasal NO levels in patients with olfactory dysfunction due to different causes.Study Design:Prospective study in a university clinic setting (tertiary referral center).Methods:Posttraumatic (n = 11), idiopathic (n = 13), and sinonasal‐related olfactory‐impaired patients (n = 55) were compared with healthy subjects (n = 11). Nasal NO levels, olfactory testing (Sniffin' Sticks), and rhinosinusitis questionnaires (Short‐Form 36, Sinonasal Outcome Test 22, Rhinosinusitis Disability Index) were obtained.Results:No significant difference in nasal NO levels were found between the different olfactory dysfunction causes. Nasal NO correlated negatively with age and positively with overall olfactory function, olfactory discrimination, and identification but not with olfactory thresholds. The more nasal symptoms prevailed in the Rhinosinusitis Disability Index, the lower the nasal NO.Conclusions:Nasal NO levels do not allow for discrimination between olfactory loss due to various etiologies based on the present data. Nasal NO production seems to decrease with age and also seems to be associated to overall olfactory function and in particular to central nervous system tasks such as olfactory discrimination and identification but not to olfactory thresholds. These findings raise questions about the link and interaction between olfactory function and nasal NO.
Decreased SPLUNC1 expression is associated with pseudomonas infection in surgically treated chronic rhinosinusitis patients who may require repeated sinus surgery
Authors: Tsou Yung‐An , Chen Chuan‐Mu , Lin Tzu‐Chieh , Hu Fang‐Wei , Tai Chin‐Jann , Chen Hui‐Chen , Yeh Te‐Huei , Harn Horng‐Jyh , Tsai Ming‐Hsui , Jan Chia‐Ing 
Published: January 2013

AbstractObjectives/Hypothesis:Chronic rhinosinusitis colonized with Pseudomonas aruginosa is difficult to treat and is related to biofilm formation. Repeated sinus surgery is often required for these patients. Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) is an epithelium‐secreted protein that is involved in innate immunity and has anti‐Pseudomonas and antibiofilm functions. This study examined if SPLUNC1 expression was related to sinusitis with bacterial culture positive for Pseudomonas and the possibility of using SPLUNC1 to predict treatment outcomes for sinusitis.Study Design:Nonrandomized retrospective study.Methods:This was a retrospective study of patients at a tertiary referral center. Pseudomonas aruginosa infection was compared to clinical variables such as SPLUNC1 mRNA expression levels, immunohistochemical (IHC) stain intensity, Lund‐Mackay sinus computed tomography scores, rapid recurrent sinusitis, requirement for repeat sinus surgery, Phadiatop test results, age, gender, nasal polyp(s), and patients' presence/absence of diabetes mellitus. Comparisons between groups were performed using the χ2 test or Fisher exact test when one confronter was <5. The statistical analyses were carried out with SPSS version 13.Results:P. aeruginosa sinus infections were associated with lower sinus mucosa SPLUNC1 expression (P = .0018), weaker SPLUNC1 IHC staining intensity (P = .011), and poor postoperative outcome (i.e., need repeated sinus surgery) (P < .001). Other factors were not associated with Pseudomonas sinus infection.Conclusions:Sinusitis with positive P. aeruginosa bacterial culture is associated with decreased SPLUNC1 sinus mucosa expression. Repeated sinus surgeries are more frequently needed for these patients.
Encapsulation of In Situ Nanoprecipitated Inorganic Materials in Confined Geometries Into a Polymer Shell Using Inverse Miniemulsion
Authors: Hamberger Anika , Ziener Ulrich , Landfester Katharina 
Published: January 2013

AbstractHighly insoluble inorganic nanoparticles can be prepared in situ by precipitation inside of aqueous nanodroplets of cosonicated inverse miniemulsions containing salts, which are readily soluble in water. If the different salt droplets are fused, a hardly soluble salt is formed in the dispersed phase with a size considerably smaller than the size of the droplets. Subsequent encapsulation of the nanoparticles into a polymer shell is achieved by an interfacial polyaddition reaction between a polyol present in the aqueous phase and a (second) monomer (2,4‐toluene diisocyanate (TDI)) in the continuous phase. The materials are studied by transmission electron microscopy, X‐ray diffraction, dynamic light scattering (DLS), and TGA.
Molecular Dynamics Investigation on the Inhibition of MDM2‐p53 Interaction by Polyphenols
Authors: Verma Sharad , Singh Amit , Mishra Abha 
Published: January 2013

AbstractInhibition of the MDM2‐p53 interaction has become a new therapeutic strategy to activate wild type p53 in tumors. Quercetin and taxifolin bind to p53 binding hydrophobic groove of MDM2, and alter the conformation of groove as evidenced by 65 ns molecular dynamics simulation. Quercetin showed hydrogen bonding with Gly 16, Ser 17, Phe 55 and Val 93 along with π–π interaction with His96 and π–σ with Phe 55. Taxifolin also showed similar interactions except π–σ interaction with Phe 55. Further, we found that binding of ligands lead to the dissociation of MDM2–p53 complex. These ligands form stable hydrophobic interactions with MDM2 which led to complete disruption of MDM2‐p53 hydrophobic interactions and dissociation of p53 from the complex. It was found that the π–π stacking between Tyr 51 of MDM2 and ligands is the critical event in MDM2‐p53 dissociation.
Accelerated MRI by SPEED with generalized sampling schemes
Authors: Jin Zhaoyang , Xiang Qing‐San 
Published: January 2013

PurposeTo enhance the fast imaging technique of skipped phase encoding (PE) and edge deghosting (SPEED) for more general sampling options, and thus more flexibility in implementations and applications.MethodsSPEED uses skipped PE steps to accelerate MRI scan. Previously, the PE skip size was chosen from prime numbers only. This restriction has been relaxed in this study to allow choice of any integers rather than merely prime numbers. Various sampling patterns were studied under all possible combinations of PE skip size and PE shifts. A criterion based on the rank values of ghost phasor matrices was introduced to evaluate SPEED reconstruction.ResultsThe reconstruction quality was found to correlate with the rank value of the ghost phasor matrix and the skipped PE size N. A low‐rank value indicates a singular matrix that causes failure of the SPEED reconstruction. Composite numbers combined with appropriately chosen PE shifts yielded satisfactory reconstruction results.ConclusionWith properly chosen PE shifts, it was found that any integers, including both prime numbers and composite numbers, could be used as PE skip size for SPEED. This finding allows much more flexible data acquisition options that may lead to more freedom in practical implementations and applications. Magn Reson Med 70:1674–1681, 2013. © 2013 Wiley Periodicals, Inc.