bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple Site‐Specific Installations of Nε‐Monomethyl‐L‐Lysine into Histone Proteins by Cell‐Based and Cell‐Free Protein Synthesis
Authors: Yanagisawa Tatsuo , Takahashi Mihoko , Mukai Takahito , Sato Shin , Wakamori Masatoshi , Shirouzu Mikako , Sakamoto Kensaku , Umehara Takashi , Yokoyama Shigeyuki 
Published: July 2014

AbstractLysine methylation is one of the important post‐translational modifications of histones, and produces an Nε‐mono‐, di‐, or trimethyllysine residues. Multiple and site‐specific lysine methylations of histones are essential to define epigenetic statuses and control heterochromatin formation, DNA repair, and transcription regulation. A method was previously developed to build an analogue of Nε‐monomethyllysine, with cysteine substituting for lysine. Here, we have developed a new method of preparing histones bearing multiple Nε‐monomethyllysine residues at specified positions. Release factor 1‐knockout (RFzero) Escherichia coli cells or a cell‐free system based on the RFzero cell lysate was used for protein synthesis, as in RFzero cells UAG is redefined as a sense codon for non‐canonical amino acids. During protein synthesis, a tert‐butyloxycarbonyl‐protected Nε‐monomethyllysine analogue is ligated to Methanosarcina mazei pyrrolysine tRNA (tRNAPyl) by M. mazei pyrrolysyl‐tRNA synthetase mutants, and is translationally incorporated into one or more positions specified by the UAG codon. Protecting groups on the protein are then removed with trifluoroacetic acid to generate Nε‐monomethyllysine residues. We installed Nε‐monomethyllysine residues at positions 4, 9, 27, 36, and/or 79 of human histone H3. Each of the Nε‐monomethyllysine residues within the produced histone H3 was recognized by its specific antibody. Furthermore, the antibody recognized the authentic Nε‐monomethyllysine residue at position 27 better than the Nε‐monomethyllysine analogue built with cysteine. Mass spectrometry analyses also confirmed the lysine modifications on the produced histone H3. Thus, our method enables the installation of authentic Nε‐monomethyllysines at multiple positions within a protein for large‐scale production.
The Effect of Water on Furan Conversion over ZSM‐5
Authors: Gilbert Christopher J. , Espindola Juliana S. , Conner William C. , Trierweiler Jorge O. , Huber George W. 
Published: July 2014

AbstractCatalytic fast pyrolysis is a method for converting lignocellulosic biomass into renewable aromatics and olefins. Water is a byproduct of this reaction and is also present in the biomass feed. As the water partial pressure is increased from 0 to 212 Torr (0 to 28 kPa), there is an increase in furan conversion from 43.8 to 84.8 % over ZSM‐5. The CO2 and propylene yields also increase from 0.7 to 16.4 % and 2.9 to 44.9 %, respectively, as the water partial pressure increases. Water partial pressures in an industrial catalytic fast pyrolysis reactor should be within the range of water partial pressures used in this study. These results demonstrate that the presence of water promotes hydrolysis reactions in the gas‐phase conversion of furanic pyrolysis vapors over zeolite catalysts.
Comparison of Hydrogen Elimination from Molecular Zinc and Magnesium Hydride Clusters
Authors: Intemann Julia , Sirsch Peter , Harder Sjoerd 
Published: July 2014

AbstractIn analogy to the previously reported tetranuclear magnesium hydride cluster with a bridged dianionic bis‐β‐diketiminate ligand, a related zinc hydride cluster has been prepared. The crystal structures of these magnesium and zinc hydride complexes are similar: the metal atoms are situated at the corners of a tetrahedron in which the vertices are bridged either by dianionic bis‐β‐diketiminate ligands or hydride ions. Both structures are retained in solution and show examples of H−⋅⋅⋅H−NMR coupling (Mg: 8.5 Hz; Zn: 16.0 Hz). The zinc hydride cluster [NN‐(ZnH)2]2thermally decomposes at 90 °C and releases 1.8 equivalents of H2. In contrast to magnesium hydride clusters, there is no apparent relationship between cluster size and thermal decomposition temperature for the zinc hydrides. DFT calculations reproduced the structure of the zinc hydride cluster reasonably well and charge density analysis showed no bond paths between the hydride ions. This contrasts with calculations on the analogous magnesium hydride cluster in which a counter‐intuitive H−⋅⋅⋅H−bond path was observed. Forcing a reduced H−⋅⋅⋅H−distance in the zinc hydride cluster, however, gave rise to a H−⋅⋅⋅H−bond path. Such weak interactions could play a role in H2desorption. The presumed molecular product after H2release, a Zn(I) cluster, could not be characterized experimentally but DFT calculations predicted a cluster with two localized ZnZn bonds.
Catalytic Functionalization of Methane and Light Alkanes in Supercritical Carbon Dioxide
Authors: Fuentes M. Ángeles , Olmos Andrea , Muñoz Bianca K. , Jacob Kane , González‐Núñez M. Elena , Mello Rossella , Asensio Gregorio , Caballero Ana , Etienne Michel , Pérez Pedro J. 
Published: July 2014

AbstractThe development of catalytic methods for the effective functionalization of methane yet remains a challenge. The best system known to date is the so‐called Catalytica Process based on the use of platinum catalysts to convert methane into methyl bisulfate with a TOF rate of 10−3 s. In this contribution, we report a series of silver complexes containing perfluorinated tris(indazolyl)borate ligands that catalyze the functionalization of methane into ethyl propionate upon reaction with ethyl diazoacetate (EDA) by using supercritical carbon dioxide (scCO2) as the reaction medium. The employment of this reaction medium has also allowed the functionalization of ethane, propane, butane, and isobutane.
Solution‐Processible Brilliantly Luminescent EuIII Complexes with Host‐Featured Phosphine Oxide Ligands for Monochromic Red‐Light‐Emitting Diodes
Authors: Wang Jianzhe , Han Chunmiao , Xie Guohua , Wei Ying , Xue Qin , Yan Pengfei , Xu Hui 
Published: July 2014

AbstractA series of solution‐processible electroluminescent (EL) Eu3+ complexes were constructed with a self‐host strategy, in which neutral ligands were employed as functionalized bidentate phosphine oxide (PO) ligands named DPEPOArn (DPEPO=bis(2‐(diphenylphosphino)phenyl) ether oxide). The solubility of these complexes was dramatically improved owing to the increased ratios of organic components. This further enhanced the antenna effect of these ligands in both singlet and triplet energy‐transfer processes to support high photoluminescent quantum yields (PLQYs) up to 86 % for their Eu3+ complexes, which is outstanding among conjugated Eu3+ complexes. Density function theory (DFT) simulations and electrochemical analysis further verified the contributions of DPEPOArn to the carrier injecting/transporting ability of the complexes. In this sense, these functionalized PO ligands served as hosts in optoelectronic processes, which rendered the self‐host feature of their Eu3+ complexes. With the enhanced electrical properties, the spin‐coated single‐layer organic light‐emitting diodes (OLEDs) of these complexes achieved improved low driving voltages, such as onset voltages about 6 V, compared to their Eu3+‐contained red‐emitting polymeric analogues. [Eu(DBM)3DPEPODPNA2] (DBM=1,3‐diphenylpropane‐1,3‐dione, DPNA=diphenylnaphthylamine) with the most enhanced electrical properties and suitable frontier molecular orbital (FMO) and triplet state locations endowed its devices with the biggest maximum luminance of >90 cd m−2 and the highest EL efficiencies. This work verified the potential of small molecular EL Eu3+ complexes for solution‐processed OLEDs through rational function integrations.
One‐Pot Decarboxylative Acylation of N‐, O‐, S‐Nucleophiles and Peptides with 2,2‐Disubstituted Malonic Acids
Authors: Lebedyeva Iryna O. , Biswas Suvendu , Goncalves Kevin , Sileno Sean M. , Jackson Ashton R. , Patel Kunal , Steel Peter J. , Katritzky Alan R. 
Published: July 2014

AbstractMonocarbonyl activation of 2,2‐disubstituted malonic acids with benzotriazole leads to decarboxylation of one of the carboxy groups and formation of a CH bond. Intermediate carbonyl benzotriazoles then readily acylate nucleophilic reagents and peptides resulting in libraries of conjugates and peptidomimetics.
Estimation of Enantiomeric Impurity in Piperidin‐3‐Amine by Chiral HPLC With Precolumn Derivatization
Authors: Raghunadha Babu Chittabathina Venkata , Vuyyuru Nagi Reddy , Padmaja Reddy K. , Suryanarayana M.V. , Mukkanti K. 
Published: July 2014

ABSTRACTA simple, precise, accurate, robust chiral high‐performance liquid chromatographic (chiral HPLC) method was developed for estimation of (S)‐piperidin‐3‐amine (S‐isomer) in (R)‐piperidin‐3‐amine dihydrochloride (R‐AMP). As AMP is a high‐melting solid and nonchromophoric compound, development of a suitable chiral method is a challenging task. The proposed chiral HPLC‐UV method involves a precolumn derivatization technique with para toluene sulphonyl chloride (PTSC) in the presence of a base to introduce chromophore into analytes. It utilizes chiralpak AD‐H column with a simple mobile phase of 0.1% diethyl amine in ethanol with a 0.5 mL/min flow rate. Analytes were monitored by using a UV detector at 228 nm. The resolution between the two enantiomers was more than 4.0. The developed method was validated as per current International Conference on Harmonization (ICH) guidelines.Chirality 26:775–779, 2014. © 2014 Wiley Periodicals, Inc.
Impact of lung cancer screening results on participant health‐related quality of life and state anxiety in the National Lung Screening Trial
Authors: Gareen Ilana F. , Duan Fenghai , Greco Erin M. , Snyder Bradley S. , Boiselle Phillip M. , Park Elyse R. , Fryback Dennis , Gatsonis Constantine 
Published: July 2014

BACKGROUNDLow‐dose computed tomography (LDCT) lung screening has been associated with a 20% reduction in lung cancer mortality. A major barrier to the adoption of lung screening is the potential negative psychological impact of a false‐positive (FP) screen, occurring in 20% to 50% of those screened. The objective of this study was to assess the impact of abnormal findings on health‐related quality of life (HRQoL) and anxiety in the American College of Radiology (ACRIN)/National Lung Screening Trial (NLST).METHODSThe NLST was a randomized screening trial comparing LDCT with chest X‐ray screening (CXR). This study was part of the original protocol. A total of 2812 participants at 16 of 23 ACRIN sites who had baseline HRQoL assessments were asked to complete the Short Form‐36 and the State Trait Anxiety Inventory (form Y‐1) questionnaires to assess short‐term (1 month) and long‐term (6 months) effects of screening. FP were lung cancer–free at 1 year, and true‐positives (TP) were not.RESULTSOf the total participants, 1024 (36.4%) participants were FP, 63 (2.2%) were TP, 344 (12.2%) had significant incidental findings (SIFs), and 1381 (49.1%) had negative screens. Participants had been randomized to LDCT (n = 1947) and CXR (n = 865). Short‐term and long‐term HRQoL and state anxiety did not differ across participants with FP, SIF, or negative screens. Short‐term and long‐term HRQoL were lower and anxiety was higher for TP participants compared to participants with FP, SIF, and negative screens.CONCLUSIONSIn a large multicenter lung screening trial, participants receiving a false‐positive or SIF screen result experienced no significant difference in HRQoL or state anxiety at 1 or at 6 months after screening relative to those receiving a negative result. Cancer 2014;120:3401–3409. © 2014 The Authors. Cancer published by Wiley Periodicals, Inc. on behalf of American Cancer Society.
An automated approach for estimating the memory footprint of nonlinear data objects
Authors: Dreßler Sebastian , Steinke Thomas 
Published: July 2014

SummaryCurrent programming models for heterogeneous devices with disjoint physical memory spaces require explicit allocation of device memory and explicit data transfers. While it is quite easy to manually implement these operations for linear data objects like arrays, this task becomes more difficult for nonlinear objects, for example, linked lists or multiple inherited classes. The difficulties arise because of memory requirements known only at run‐time and the composition of complex data structures from basic types. In this paper, we present a novel method to build a graph‐based static data‐type description that is used to create code for injectable functions that automatically determine the memory footprint of data objects at run‐time. Our approach is extensible to implement automatically generated optimized data transfers across physical memory spaces. Copyright © 2014 John Wiley & Sons, Ltd.
Real‐Time Observation of Liposome Bursting Induced by Acetonitrile
Authors: Yoshida Kazunari , Horii Keitaro , Fujii Yasuhiro , Nishio Izumi 
Published: July 2014

AbstractWe show the bursting process of dioleoylphosphatidylcholine (DOPC) liposomes in response to the addition of acetonitrile, a small toxic molecule widely used in the fields of chemistry and industry. The percentage of destroyed liposomes is reduced upon decreasing the acetonitrile fraction in the aqueous solution and vesicle bursting is not observed at volume ratios of 4:6 and below. This indicates that a high fraction of acetonitrile causes the bursting of liposomes, and it is proposed that this occurs through insertion of the molecules into outer leaflet of the lipid bilayer. The elapsed time between initial addition of acetonitrile and liposome bursting at each vesicle is also measured and demonstrated to be dependent on the volume fraction of acetonitrile and the vesicle size.
Next‐Generation, High‐Energy‐Density Redox Flow Batteries
Authors: Huang Qizhao , Wang Qing 
Published: July 2014

AbstractRedox flow batteries are experiencing rapid growth for stationary energy‐storage applications. To satisfy the demand for wider applications, however, improved energy density of redox flow batteries is desperately required. Past and present efforts to increase the energy density are briefly surveyed herein and several strategies are explored.
Efficient Organic Sensitizers with Pyridine‐N‐oxide as an Anchor Group for Dye‐Sensitized Solar Cells
Authors: Wang Lei , Yang Xichuan , Zhao Jianghua , Zhang Fuguo , Wang Xiuna , Sun Licheng 
Published: July 2014

AbstractFive organic dyes with pyridine‐N‐oxide as the anchor group and electron acceptor have been synthesized and applied in dye‐sensitized solar cells (DSSCs). Benzothiadiazole was introduced in the conjugation system to increase the electron withdrawing properties, FTIR spectra showed that the coordination was between the pyridine‐N‐oxide and the Brønsted acid site on the TiO2 surface. The relationship between different dye structures and the performance of the DSSCs was investigated systematically. The location of the thiophene unit was studied, and the direct linkage of benzothiadiazole with pyridine‐N‐oxide was beneficial to broaden the absorption. The donor–acceptor–acceptor‐configured dye WL307, which has 2‐ethylhexyloxy chains in the donor part, showed the best efficiency of 6.08 % under 100 mW cm−2 light illumination. The dye series showed a fairly good stability during the one month test period.
Unconventional Pore and Defect Generation in Molybdenum Disulfide: Application in High‐Rate Lithium‐Ion Batteries and the Hydrogen Evolution Reaction
Authors: Zhang Kan , Kim Hwan‐Jin , Lee Jeong‐Taik , Chang Gee‐Woo , Shi Xinjian , Kim Wanjung , Ma Ming , Kong Ki‐jeong , Choi Jae‐Man , Song Min‐Sang , Park Jong Hyeok 
Published: July 2014

AbstractA 2H‐MoS2 (H=hexagonal) ultrathin nanomesh with high defect generation and large porosity is demonstrated to improving electrochemical performance, including in lithium‐ion batteries (LIBs) and the hydrogen evolution reaction (HER), with the aid of a 3D reduced graphene oxide (RGO) scaffold as fast electron and ion channels. The 3D defect‐rich MoS2 nanomesh/RGO foam (Dr‐MoS2 Nm/RGO) can be easily obtained through a one‐pot cobalt acetate/graphene oxide (GO) co‐assisted hydrothermal reaction, in which GO, cobalt and acetate ions are co‐morphology‐controlling agents and defect inducers. As an anode material for LIBs, Dr‐MoS2 Nm/RGO has only a 9 % capacity decay at a 10 C discharge rate versus 0.2 C with stable cyclability at the optimized composition (5 wt % RGO to MoS2 and 2 mol % Co to Mo), and significantly achieves 810 mA h g−1 at a high current density of 9.46 A g−1 over at least 150 cycles. Moreover, Dr‐MoS2 Nm/RGO exhibits superior activity for the HER with an overpotential as low as 80 mV and a Tafel slope of about 36 mV per decade. In contrast to the MoS2 nanosheet/RGO (MoS2 Ns/RGO), which is synthesized in the absence of cobalt ions, Dr‐MoS2 Nm/RGO provides high interconnectivity for efficient lithium‐ion transport, and rich defects as electrochemically active sites. DFT is used to prove the existence of rich defects due to anion replacement to become a CoMoS atomic structure, releasing inert basal planes to active sites.
Second messenger signaling for neuronal polarization: Cell mechanics‐dependent pattern formation
Authors: Guo Chin‐Lin , Cheng Pei‐Lin 
Published: July 2014

ABSTRACTNeuronal polarization is a critical step in the neuronal morphogenesis. Despite the identification of several evolutionarily conserved factors for neural polarization, the exact mechanisms by which cells initiate and maintain polarity remain to be characterized. Here, we review the recent progress on the roles of second messengers, specifically the cyclic nucleotides and membrane‐associated phospholipids, in the initiation, propagation, and integration of polarization signals, and propose an inhibitor‐free model for neural polarization. The characteristic features of neuron polarization include the formation of single axon and multiple dendrites. These features involve chemical and mechanical mechanisms such as reaction‐diffusion and tug‐of‐war, by which second messengers can act in concert to initiate and stabilize the cellular asymmetry. Nevertheless, biochemical factors eliciting the long‐range inhibition remain ambiguous. Thus, we provide a simple, inhibitor‐free model that can incorporate known cytochemical and cytomechanical factors, and produce features of neuronal polarization in environments provided with minimized extracellular regulators. © 2014 The Authors Developmental Neurobiology Published by Wiley Periodicals, Inc. Develop Neurobiol 75: 388–401, 2015
Ask and you shall receive: Desire and receipt of feedback via Facebook predicts disordered eating concerns
Authors: Hummel Alexandra C. , Smith April R. 
Published: July 2014

ABSTRACTObjectiveThe current study examined whether certain types of Facebook content (i.e., status updates, comments) relate to eating concerns and attitudes.MethodWe examined the effects of seeking and receiving negative feedback via Facebook on disordered eating concerns in a sample of 185 undergraduate students followed for approximately 4 weeks.ResultsResults indicated that individuals with a negative feedback seeking style who received a high number of comments on Facebook were more likely to report disordered eating attitudes four weeks later. Additionally, individuals who received extremely negative comments in response to their personally revealing status updates were more likely to report disordered eating concerns four weeks later.DiscussionResults of the current study provide preliminary evidence that seeking and receiving negative feedback via social networking sites can increase risk for disordered eating attitudes, and suggest that reducing maladaptive social networking usage may be an important target for prevention and intervention efforts aimed at reducing disordered eating attitudes. © 2014 Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:436–442)
Pain referents used to respond to the Pain Catastrophizing Scale
Authors: Kapoor S. , Thorn B.E. , Bandy O. , Clements K.L. 
Published: July 2014

AbstractBackgroundPain catastrophizing has emerged as a highly important construct in pain research. The Pain Catastrophizing Scale (PCS) is a widely used self‐report measure used to determine a person's level of pain catastrophizing, assumed to be associated with an ongoing, recalled or anticipated pain experience. In practice, instructions for self‐reporting catastrophizing typically do not provide a specific pain referent, even when assessing patients with chronic pain. Researchers have noted that it is not known what type of pain participants are referring to when responding to a catastrophizing questionnaire.MethodIn the current study, 182 presumably healthy undergraduate students completed the PCS followed by a query regarding the pain referent used to complete the scale. In addition, they were asked if they have ever experienced chronic pain and to list their worst pain experience.ResultsThe most commonly used primary referents included pain due to acute injury (26.4%), headache (18.0%) and general physical pain (11.5%). The type of primary referent and the number of referents did not influence the catastrophizing scores. However, the catastrophizing scores were influenced by the context of the primary pain referent, i.e., whether the primary pain referent was non‐chronic worst pain, both chronic and worst pain, chronic pain or unrelated to either chronic or worst pain. Notably, a larger than expected proportion of participants reported having experienced chronic pain (44.5%; n = 81).ConclusionThe examination of pain referents while responding to a catastrophizing measure would add to our understanding of a person's pain experience and related catastrophic cognitions.
Efficient and Green Synthesis of N‐aryl Pyrroles Catalyzed by Ionic Liquid [H‐NMP][HSO4] in Water at Room Temperature
Authors: Naeimi Hossein , Dadaei Mahla 
Published: July 2014

AbstractA suitable and efficient method for the synthesis of N‐aryl pyrroles by using 2,5‐dimethoxy tetrahydrofuran and several primary aromatic amines in the presence of the catalytic amount of [H‐NMP][HSO4] under room temperature was described. This method has the advantages such as; easy reaction workup, absolutely separated of catalyst from the reaction mixture and smoothly recyclability of catalyst. In this reaction, N‐substituted pyrroles were obtained as desired products in excellent yields and short reaction times via green and one‐pot procedure.
Solvent extraction of Nd using organo‐phosphorus extractants from chloride media
Authors: Padhan Eliza , Sarangi Kadambini 
Published: July 2014

AbstractINTRODUCTIONA comparative study on solvent extraction of neodymium using both acidic and neutral form of phosphonic, phosphinic and thiophosphinic acid extractants from chloride media has been carried out. The extractants used for the study were PC88A, Cyanex 272 and Cyanex 302.RESULTSIn this study the effect of different parameters such as equilibrium pH, extractant concentration, A:O phase ratio and temperature on Nd extraction was investigated. With increasing pH and extractant concentration the percentage extraction of Nd increased for all these extractants. The percentage extraction of Nd was maximum with NaCyanex 302. So after optimizing the extraction and stripping conditions, the McCabe–Thiele plots for neodymium extraction and stripping were constructed with 0.025 mol L−1 NaCyanex 302 at pH 4.2 and 0.08 (v/v)% sulphuric acid, respectively.CONCLUSIONThe McCabe–Thiele plots indicated two stages for both extraction and stripping at A:O ratio of 1. The extracted species was determined to be NdA3.5HA. The thermodynamic parameters such as ΔH, ΔS and ΔG were calculated. The loading capacity of NaCyanex 302 for Nd and the synergistic coefficient of NaCyanex302 with NaCyanex272 and NaPC88A were determined. The effect of different salts such as NaCl, NaNO3 and Na2SO4 on extraction of Nd was also studied. © 2014 Society of Chemical Industry