bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of nutrition‐based prehabilitation on the postoperative outcomes of patients with esophagogastric cancer undergoing surgery: A systematic review and meta‐analysis
Authors: Shen Yi , Cong Zhuangzhuang , Ge Qiyue , Huang Hairong , Wei Wei , Wang Changyong , Jiang Zhisheng , Wu Yuheng 
Published: July 2024

AbstractBackgroundMeta‐analyses have primarily focused on the effects of exercise‐based prehabilitation on postoperative outcomes and ignored the role of nutritional intervention. In this study, we filled this gap by investigating the effect of nutrition‐based prehabilitation on the postoperative outcomes of patients who underwent esophagectomy and gastrectomy.MethodsFive electronic databases, namely, PubMed, the Web of Science, Embase, Cochrane Library, and CINAHL, were searched. Adults diagnosed with esophagogastric cancer who were scheduled to undergo surgery and had undergone uni‐ or multimodal prehabilitation, with at least a week of mandatory nutritional intervention, were included. Forest plots were used to extract and visualize the data from the included studies. The occurrence of any postoperative complication was considered the primary endpoint.ResultsEight studies met the eligibility criteria, with five randomized controlled trials (RCTs) and three cohort studies. In total, 661 patients were included. Any prehabilitation, that is, unimodal (only nutrition) and multimodal prehabilitation, collectively decreased the risk of any postoperative complication by 23% (95% confidence interval [CI] = 0.66–0.90). A similar effect was exclusively observed for multimodal prehabilitation (risk ratio [RR] = 0.78, 95% CI = 0.66–0.93); however, it was not significant for unimodal prehabilitation. Any prehabilitation significantly decreased the length of hospital stay (LOS) (weighted mean difference = −0.77, 95% CI = −1.46 to −0.09).ConclusionsNutrition‐based prehabilitation, particularly multimodal prehabilitation, confers protective effects against postoperative complications after esophagectomy and gastrectomy. Our findings suggest that prehabilitation slightly decreases LOS; however, the finding is not clinically significant. Therefore, additional rigorous RCTs are warranted for further substantiation.
Not to blame, but still responsible: Negotiating social representations of neoliberal feminism amongst urban middle‐class Indians
Authors: D'silva Keshia 
Published: July 2024

AbstractIndian gender advocacy has been criticised for promoting individualistic neoliberal solutions to issues requiring structural change. Such solutions are most accessible to middle‐class women and their dominance in gender advocacy is attributed to the middle‐class viewership of campaigns. Few studies have researched how this intended audience responds to such messages. As patriarchy benefits men, it is also important to understand how gender intersects with social class to shape responses. Accordingly, this research explores the reception of neoliberal feminist ideals in Indian gender advocacy amongst an urban middle‐class audience of 25 participants, comprising of 13 women and 12 men in Bengaluru, India. The theoretical and methodological framework is the social representations theory. Participants viewed three campaign videos and undertook semi‐structured interviews. Thematic analysis was employed to explore their perceptions of the causes and solutions to the issues portrayed in the campaigns, leading to three themes being identified. In the theme of ‘individuals’, participants held women responsible for resisting oppression. In the theme of ‘culture’, participants viewed culture as a cause of patriarchy, with cultural change seen as a solution. In the theme of ‘institutions’, participants discussed institutional sexism but were pessimistic about institutional reform. These findings suggest that whilst patriarchy is blamed on cultural norms and institutional failure, solutions are often conceived in terms of self‐transformation of individual women. These results are situated in social representations' theoretical premise of themata and positioning to analyse how social locations like gender, class and regional identity were mobilised by participants to contest or maintain a neoliberal feminist ideology. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement.
tRNA‐derived fragment tRF‐30 propels diabetes‐induced retinal microvascular complications by regulating STAT3 signaling
Authors: Yuan Dongqing , Xu Yingnan , Xue Lian , Zhang Weiwei , Gu Liuwei , Liu Qinghuai 
Published: July 2024

AbstractTransfer RNA‐derived fragments (tRFs) represent a novel class of non‐coding RNA transcripts that possess specific biological functions. However, the involvement of tRFs in retinal microvascular diseases remains poorly understood. In this study, we aimed to reveal whether modulation of tRF‐30 expression could attenuate pathological retinal neovascular diseases. Our findings demonstrate a significant upregulation of tRF‐30 expression levels in both in vivo models of diabetic retinopathy (DR) and in vitro endothelial sprouting models. Conversely, inhibition of tRF‐30 expression suppressed the formation of abnormal neovascularization in the retina in vivo, while reducing the proliferation and migration activity of retinal vascular endothelial cells in vitro. We also found that tRF‐30 modulates retinal neovascularization through the tRF‐30/TRIB3/signal transducer and activated transcription 3 signaling pathway. Furthermore, we validated a significant upregulation of tRF‐30 expression levels in the vitreous humor of DR patients, with high levels of both validity and specificity in diagnostic testing. Collectively, our findings highlight a pro‐angiogenic role for tRF‐30 in DR. Intervening in the tRF‐30 signaling pathway may represent a promising prevention and treatment strategy for retinal angiogenesis.
A hidden proteome encoded by circRNAs in human placentas: Implications for uncovering preeclampsia pathogenesis
Authors: Zhao Huanqiang , Xiong Yu , Zhou Zixiang , Xu Qixin , Zi Yang , Zheng Xiujie , Chen Shiguo , Xiao Xirong , Gong Lili , Xu Huangfang , Liu Lidong , Lu Huiqing , Cui Yutong , Shao Shuyi , Zhang Jin , Ma Jing , Zhou Qiongjie , Ma Duan , Li Xiaotian 
Published: July 2024

AbstractBackgroundCircRNA‐encoded proteins (CEPs) are emerging as new players in health and disease, and function as baits for the common partners of their cognate linear‐spliced RNA encoded proteins (LEPs). However, their prevalence across human tissues and biological roles remain largely unexplored. The placenta is an ideal model for identifying CEPs due to its considerable protein diversity that is required to sustain fetal development during pregnancy. The aim of this study was to evaluate circRNA translation in the human placenta, and the potential roles of the CEPs in placental development and dysfunction.MethodsMultiomics approaches, including RNA sequencing, ribosome profiling, and LC‐MS/MS analysis, were utilised to identify novel translational events of circRNAs in human placentas. Bioinformatics methods and the protein bait hypothesis were employed to evaluate the roles of these newly discovered CEPs in placentation and associated disorders. The pathogenic role of a recently identified CEP circPRKCB119aa in preeclampsia was investigated through qRT‐PCR, Western blotting, immunofluorescence imaging and phenotypic analyses.ResultsWe found that 528 placental circRNAs bound to ribosomes with active translational elongation, and 139 were translated to proteins. The CEPs showed considerable structural homology with their cognate LEPs, but are more stable, hydrophobic and have a lower molecular‐weight than the latter, all of which are conducive to their function as baits. On this basis, CEPs are deduced to be closely involved in placental function. Furthermore, we focused on a novel CEP circPRKCB119aa, and illuminated its pathogenic role in preeclampsia; it enhanced trophoblast autophagy by acting as a bait to inhibit phosphorylation of the cognate linear isoform PKCβ.ConclusionsWe discovered a hidden circRNA‐encoded proteome in the human placenta, which offers new insights into the mechanisms underlying placental development, as well as placental disorders such as preeclampsia.Key points
A hidden circRNA‐encoded proteome in the human placenta was extensively identified and systematically characterised.
The circRNA‐encoded proteins (CEPs) are potentially related to placental development and associated disorders.
A novel conserved CEP circPRKCB119aa enhanced trophoblast autophagy by inhibiting phosphorylation of its cognate linear‐spliced isoform protein kinase C (PKC) β in preeclampsia.
Detection of nonsteroidal and steroidal selective androgen receptor modulators in equine hair after oral administrations
Authors: So Yat‐Ming , Kong Fred Ka‐Wai , Kwok Wai Him , Kwok Karen Ying , Wan Terence See Ming , Ho Emmie Ngai‐Man 
Published: July 2024

AbstractThis paper describes the detections of nonsteroidal and steroidal selective androgen receptor modulators (SARMs), namely, RAD140 and YK‐11, in mane hair collected from horses having been orally administered with the respective drugs. SARMs are potent anabolic agents with a high potential of misuse in horseracing and equestrian sports, and the misuses of RAD140 and YK‐11 in human sports have been reported. To better control the misuse of RAD140 and YK‐11 in horses, two separate oral administration studies of RAD140 (0.3 mg/kg daily for 3 days) and YK‐11 (0.2 mg/kg daily for 3 days) were previously conducted to investigate their metabolism and to identify target analyte(s) with the longest detection time in urine and plasma for doping control. In this work, segmental analyses of post‐administration hair samples have revealed that (i) RAD140 and YK‐11 could be detected in horse mane after oral administration and (ii) internal incorporation of RAD140 into hair via bloodstream and external incorporation through sweat or sebum were both observed, whereas YK‐11 was primarily incorporated into hair via sweat or sebum.
Disruption of the creb3l1 gene causes defects in caudal fin regeneration and patterning in zebrafish Danio rerio
Authors: VanWinkle Peyton E. , Lee Eunjoo , Wynn Bridge , Nawara Tomasz J. , Thomas Holly , Parant John , Alvarez Cecilia , Serra Rosa , Sztul Elizabeth 
Published: July 2024

AbstractBackgroundThe gene cAMP‐Responsive Element Binding protein 3‐like‐1 (CREB3L1) has been implicated in bone development in mice, with CREB3L1 knock‐out mice exhibiting fragile bones, and in humans, with CREB3L1 mutations linked to osteogenesis imperfecta. However, the mechanism through which Creb3l1 regulates bone development is not fully understood.ResultsTo probe the role of Creb3l1 in organismal physiology, we used CRISPR‐Cas9 genome editing to generate a Danio rerio (zebrafish) model of Creb3l1 deficiency. In contrast to mammalian phenotypes, the Creb3l1 deficient fish do not display abnormalities in osteogenesis, except for a decrease in the bifurcation pattern of caudal fin. Both, skeletal morphology and overall bone density appear normal in the mutant fish. However, the regeneration of caudal fin postamputation is significantly affected, with decreased overall regenerate and mineralized bone area. Moreover, the mutant fish exhibit a severe patterning defect during regeneration, with a significant decrease in bifurcation complexity of the fin rays and distalization of the bifurcation sites. Analysis of genes implicated in bone development showed aberrant patterning of shha and ptch2 in Creb3l1 deficient fish, linking Creb3l1 with Sonic Hedgehog signaling during fin regeneration.ConclusionsOur results uncover a novel role for Creb3l1 in regulating tissue growth and patterning during regeneration.
Vitamin D in Type 2 Diabetes and Its Correlation With Heat Shock Protein 70, Ferric Reducing Ability of Plasma, Advanced Oxidation Protein Products and Advanced Glycation End Products
Authors: Hashemi Nazanin , Karimpour Reyhan Sahar , Qahremani Reihane , Seifouri Kiana , Tavakoli Meraj , Seyedi Seyed Arsalan , Ghaemi Farahnaz , Abbaszadeh Mahsa , Esteghamati Alireza , Nakhjavani Manouchehr , Mirmiranpour Hossein , Rabizadeh Soghra 
Published: July 2024

ABSTRACTAimTo investigate the association between vitamin D3 level and oxidative stress biomarkers such as Heat Shock Protein 70 (HSP70), ferric reducing ability of plasma (FRAP), advanced oxidation protein products (AOPP) and advanced glycation end products (AGEs) in patients with Type 2 diabetes.MethodIn this cross‐sectional study, 54 patients including 32 females and 22 males with a mean age of 54.92 ± 11.37 years with T2D attending the diabetes clinic from 2021 to 2022 were included. According to the average level of vitamin D in this population (14.91), they were divided into two groups with vitamin D ≤15 ng/mL and vitamin D >15 ng/mL. Multivariate regression analysis was conducted to evaluate the relationship between vitamin D and AOPP, HSP and FRAP parameters. The correlation between vitamin D and other variables was evaluated via the Pearson correlation test.ResultVitamin D level had a positive relation with FRAP (β = 0.32, p = 0.017) and HSP (β = 0.39, p = 0.003), but had a negative relation with AOPP (β = −0.30, p = 0.02). The level of 2hPP also had a negative relation with the level of vitamin D (β = −0.33, p = 0.03). There was not any relationship between the level of vitamin D and AGEs or other variables. After adjusting for multiple confounders for the multivariate regression model, HSP remained significant.ConclusionThis research indicates the relationship between vitamin D levels and oxidative stress biomarkers in patients with Type 2 diabetes.
Network dynamics of self‐compassion, anxiety, and depression during eating disorder therapy
Authors: Kopland Maren C. G. , Vrabel KariAnne , Landt Margarita Slof‐Op 't , Hoffart Asle , Johnson Sverre Urnes , Giltay Erik J. 
Published: July 2024

AbstractBackgroundRecovery processes during residential treatment for eating disorders, especially in patients with a history of maltreatment, are insufficiently understood. This study aimed to explore the temporal relationships among comorbid factors, including depression, anxiety, and self‐compassion, with the influence of childhood maltreatment.MethodUsing Dynamic Time Warp (DTW), weekly scores from the Symptom Checklist‐5, Eating Disorder Examination, and Self‐Compassion Scale were analysed over 12 weeks. The study generated undirected and directed networks to identify influential symptoms in a transdiagnostic sample, comparing patients with and without childhood maltreatment.ResultsThe study included 124 patients with eating disorders (ED) (97% women), mean age of 30.9 years (SD = 9.7, range 18–61 years). Diagnoses included anorexia nervosa (26%), bulimia nervosa (38%), and other specified feeding and eating disorders (36%). The directed DTW network showed that hopelessness, worrying, and restlessness had the highest out‐strength, predicting changes in self‐compassion and ED behaviour. In maltreatment cases, hopelessness and low acceptance predicted changes, while worry, restlessness, and nervousness were predictive in non‐maltreatment cases.ConclusionTemporal network analyses suggest that a change in hopelessness, worrying, and restlessness drives symptom improvement in ED behaviour and the development of self‐compassion during residential treatment. These processes vary between patients with and without a history of childhood maltreatment separately, indicating the need for further analyses.
The astrocyte α1A‐adrenoreceptor is a key component of the neuromodulatory system in mouse visual cortex
Authors: Wahis Jérôme , Akkaya Cansu , Kirunda Andre M. , Mak Aline , Zeise Karen , Verhaert Jens , Gasparyan Hayk , Hovhannisyan Sargis , Holt Matthew G. 
Published: July 2024

AbstractNoradrenaline (norepinephrine) is known to modulate many physiological functions and behaviors. In this study, we tested to what extent astrocytes, a type of glial cell, participate in noradrenergic signaling in mouse primary visual cortex (V1). Astrocytes are essential partners of neurons in the central nervous system. They are central to brain homeostasis, but also dynamically regulate neuronal activity, notably by relaying and regulating neuromodulator signaling. Indeed, astrocytes express receptors for multiple neuromodulators, including noradrenaline, but the extent to which astrocytes are involved in noradrenergic signaling remains unclear. To test whether astrocytes are involved in noradrenergic neuromodulation in mice, we employed both short hairpin RNA mediated knockdown as well as pharmacological manipulation of the major noradrenaline receptor in astrocytes, the α1A‐adrenoreceptor. Using acute brain slices, we found that the astrocytic α1A‐adrenoreceptor subtype contributes to the generation of large intracellular Ca2+ signals in visual cortex astrocytes, which are generally thought to underlie astrocyte function. To test if reduced α1A‐adrenoreceptor signaling in astrocytes affected the function of neuronal circuits in V1, we used both patch‐clamp and field potential recordings. These revealed that noradrenergic signaling through the astrocyte α1A‐adrenoreceptor is important to not only modulate synaptic activity but also to regulate plasticity in V1, through the potentiation of synaptic responses in circuits involved in visual information processing.
Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity
Authors: Zhao Zijin , You Yanbo , Hua Shaowei , Shen Xinxin , Li Lingjun , Ma Xuejun 
Published: July 2024

AbstractBackgroundFluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time PCR (qPCR) assays, but they require a complex probe design. To eliminate the addition of fluorescent probes for RAA, an EvaGreen dye‐based recombinase‐aided amplification (EvaGreen‐RAA) assay using self‐avoiding molecular recognition system (SAMRS) primers was developed.MethodsThe SAMRS primers effectively avoided the production of primer dimers, thus improving the detection sensitivity, while EvaGreen dye was used to quantitatively measure the amplified products in real time. Using Staphylococcus aureus (SA) and Listeria monocytogenes (LM) as examples, EvaGreen‐RAA with SAMRS primers was developed. As a reference and comparison, a traditional fluorescence probe RAA method and a RAA with SAMRS primers (SAMRS‐RAA) for detecting SA and LM were also investigated. Serial dilutions of recombinant plasmids were used to evaluate the sensitivity of the assays. Unenriched and enriched simulated milk samples were used to evaluate the limits of detection (LOD) of these methods. Using high‐resolution melting (HRM) was used to explore the sensitivity of the dual EvaGreen‐RAA assay.ResultsThe sensitivity of the fluorescent RAA method for detecting SA and LM was 10 copies/μL using plasmids and the sensitivity of the SAMRS‐RAA and EvaGreen‐RAA for detecting SA and LM plasmids was 1 copies/μL. The LOD values of the EvaGreen‐RAA for SA and LM in unenriched simulated milk samples were 100 and 50 CFU/mL, respectively, and the LOD value for both SA and LM using enriched simulated milk samples was 10 CFU/mL. EvaGreen‐RAA had linear amplification in real time in the range of 1–105 copies/μL of the plasmids of SA and LM. The sensitivity of the dual EvaGreen‐RAA assay for SA and LM was estimated to be 102 CFU/mL.ConclusionA real‐time quantitative EvaGreen‐RAA method for detecting SA and LM was developed, which eliminates the need to design complex RAA probes. This dye‐based RAA with SARMS primers provides a new strategy for simplifying fluorescence probe RAA and allowing the detection of multiple pathogens, which has many potential applications.
Some Rivulariaceae Rabenh. from paddy fields of Kaira District, Gujarat, India
Authors: Mahajan A. D. , Patel R. I. 
Published: July 2024

SummaryWhile exploring the blue green algae from the paddy fields of Kaira district, Gujarat, the authors collected 11 taxa of' the genus Calothrix Ag. and 4 taxa of the genus Rivularia (Roth) Ag. from Limbasi and Navagam, the maximum rice growing region of the district. All the taxa are addition to algal flora of Gujarat ecxept Rivularia aquatica De Wilde and R. hansgirgi Schmidle. Calothrix clavata West. G. S. is reported for the first time from India.
Vegetation of inland desert wadis in Egypt V. Edaphic characteristics of plant communities in wadi systems east of “Minya” Province
Authors: El‐Sharkawi H. M. , Ramadan A. A. 
Published: July 2024

SummaryThe edaphic characteristics that might be influencing the distribution of four commmunities, previously recognized in the area, were studied. This included soil particle size (texture), organic carbon, total soluble salts and major soluble anions and cations. Besides definite phytosociological identity of the communities identified, the edaphic characteristics of their habitats seem to be likewise recognizable. Certain specific chemical status in the habitats of these communities are identifiable at either the wet or dry seasons. Each community has certain distinguishable edaphic characteristics, which is quite indicative of their ecological identity, despite the rigorous disturbance of such habitats by torrential rains the area has been subject to about two years before this investigation was carried out.
Species novae Bromeliacearum V
Authors: Weber Wilhelm 
Published: July 2024

ZusammenfassungEinige neue Bromliaccae der Gattungen Aechmea, Billbergia, Canistrum, Cryplanthus, Neoregelia, Nidularium, Orthophylum, Pitcairnia, Puya, Tillandsia und Vriesca ans Argentinien, Bolivien, Brasilien, Columbrien und Peru werden beschrieben.
Viburnum colinifolium D. Don and two new infraspecific taxa
Authors: Dudley T.R. 
Published: July 2024

SummaryVihurnum cotinifolium D. Don, demonstrates two very well distinguished variants in Eastern Asia, which are described herein as var. lacei var. nov. from Pakistan and var. wallichii var. nov. from Nepal. A series of collections from Pakistan's Northeast Frontier Province and Punjab Province are recognized as intermediate between var. lacei and var. wallichii.
A nickel hyperaccumulating species of Alyssum (Cruciferae) from Spain: A. malacitanum (Rivas Goday) T. R. Dudley, comb. & stal. nov.
Authors: Dudley T. R. 
Published: July 2024

SummaryThere are two serpentinophilous and nickel‐hyperaccumulating taxa within the Alyssum serpyllifolium complex in Europe – A. pinlodasilvae T. R. Dudley from Portugal, and A. serpyllifolium subspecics malacitanam Rivas Goday from Spain. The latter is raised to full specific status as A. mulucitanum (Rivas Goday) T. R Dudley, comb. & stat. nov. on the basis of demonstrating a different nickel‐accumulation capability at 0.6% tissue concentration on dry wight basis, in comparison with the 1.44% of A. pintodasilvae and the 0.0065% of A. serpyllifolium. In conjunction with the differential and diagnostic nickel content, numerous discontinuous morphological characters clearly distinguish A. malacitilanum from the taxa of closest affinity.
Taxonomie studies in Solanum L. (and related genera) 4. Cyphomandra casana Child sp. nov. and Solanum sect. Glaucophyllum Child sect. nov.
Authors: Child Alan 
Published: July 2024

SummaryA new species of tree tomato, Cyphomandra (Section Cyphomandra) casana Child sp. nov. from near Loja, Ecuador is diagnosed and described on the basis of material grown in the New Zealand Crop Introduction Schemne (D. S. I. R.).A new infragenerie taxon for Solanum, Section Glaucophyllum Child sect. nov. is proposed to accommodate the well known and ornamental S. American species S. glaucophyllum Dunal (= S. malacoxylon Sendt.) which has hitherto been of uncertain position within the Subgenus Solanum.