bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of the Wnt Signaling Pathways during Cell Culture of Human Mesenchymal Stem Cells for Efficient Bone Regeneration
Authors: Katagiri Wataru , Yamada Yoichi , Nakamura Sayaka , Ito Kenji , Hara Kenji , Hibi Hideharu , Ueda Minoru 
Published: August 2012

AbstractTissue engineering and bone regeneration techniques using mesenchymal stem cells (MSCs) have started to be applied to the field of oral and maxillofacial surgery. Clinically, a shortened treatment time and improved efficiency are necessary because of the patients' needs and the running cost of cell culture. In the present study, the cultivation process for human MSCs (hMSCs) was examined by regulating the Wnt signaling pathway. We activated Wnt signaling with LiCl and inhibited Wnt signaling with sFRP‐3 (secreted Frizzled‐Related Protein‐3). The proliferation of LiCl‐treated hMSCs was examined by studying the cell growth rate and performing BrdU assays. Osteogenic differentiation of sFRP‐3‐treated hMSCs was examined by alizarin red staining, and osteogenic gene expression on days 7 and 14 after induction was examined by reverse‐transcription polymerase chain reaction (RT‐PCR) analysis and quantitative real‐time RT‐PCR analysis. LiCl‐treated hMSCs showed increased cell numbers and BrdU‐positive cells as compared to the untreated cells. Alizarin red staining showed early mineralization of hMSCs on day 7 of the sFRP‐3 treatment. A high expression level of the alkaline phosphatase gene on days 7 and 14 of sFRP‐3 treatment was also demonstrated. These results suggest that the regulation of the Wnt signaling pathway contributes to the increased cell numbers and the early osteogenic differentiation of hMSCs. This study supports the possibility that the regulation of the Wnt signaling pathway contributes to the development of effective and efficient bone regeneration techniques.
Objective Assessment of Erythema caused by Metal Patch Testing using an Erythema Index Meter
Authors: Takada Atsushi , Fujimoto Masaru , Ukichi Ken‐ichiro , Morimoto Mitsuaki , Tonogi Morio , Yamane Gen‐yuki , Takahashi Shin‐ichi 
Published: August 2012

AbstractThe patch test is the most reliable method for diagnosing a metal allergy. However, interpretation of patch test results depends on the experience of the investigator and on the conditions of the individual patient. In the case of patch tests for metal salts, irritant reactions such as pustular or follicular reactions are quite common. Therefore, one must be careful not to interpret irritant reactions as allergic responses, and objective standards to evaluate erythema in patch tests need to be established.In the present study, we attempted to objectively evaluate patch test results for metal salts using an erythema index meter. We used the Erythema Index Difference (EID) (patch test site erythema index (EI) minus the mean EI of healthy skin outside the patch test area and at the unit tape site) rather than the EI itself in order to eliminate the effects of the patch test unit and individual differences. We measured the EID over time in patients with suspected metal allergies to various metals. Significant differences in EID were observed among ++ assessments, + assessments and irritant reactions based on International Contact Dermatitis Research Group (ICDRG) standards. In considering changes in EID over time, allergic and irritant reactions can be discriminated with high sensitivity (73.3%) and specificity (91.2%).These results suggest that the measurement of erythema over time with an erythema index meter is useful for the objective assessment of metal patch test reactions.
A Case of Cat Cry Syndrome Associated with Cleft Lip
Authors: Kashima Koji , Igawa Kaori , Yokota Rie , Yoshioka Izumi , Sakoda Sumio 
Published: August 2012

AbstractCat cry syndrome (cri‐du‐chat syndrome) is an extremely rare condition characterized by a high shrill cry during infancy, resulting from either the deletion of the short arm of chromosome 5 or unbalanced translocation inherited from a parent. We report the case of a 1‐year‐old girl with cat cry syndrome associated with cleft lip. The patient showed a ventricular septal defect, cleft lip, growth and mental retardation, micrognathia, ptosis of the eyelids, hypertelorism, epicanthal folds, and a preauricular tag on the right side. A chromosomal study revealed the terminal deletion of chromosome 5 (46, XX, del(p14.2)ish del(5)). Repair of the cleft lip was carried out concurrently with resection of the preauricular tag, and the patient's postoperative course was uneventful.
Intraosseous Schwannoma of the Mandibular Symphysis: Report of a Case
Authors: Kawasaki Goro , Yanamoto Souichi , Yoshida Hajime , Mizuno Akio , Fujita Shuichi , Ikeda Tohru 
Published: August 2012

AbstractSchwannoma is a benign neoplasm originating from the neural sheath and occurs most commonly in the soft tissues of the head and neck. Intraosseous Schwannoma in the maxillofacial region is extremely rare. The present study reports the case of an intraosseous Schwannoma located in the mandibular symphysis of a 27‐year‐old male patient. The tumor was completely excised, and there has been no evidence of recurrence in the nearly two years since the operation. A review of the related literature is also included.
Analysis of Biological Apatite Orientation in Rat Mandibles
Authors: Nagisa Noriko , Nakano Takayoshi , Hashiguchi Norihiro , Fujitani Wataru , Umakoshi Yukichi , Shimahara Masashi 
Published: August 2012

AbstractRecently, significant progress has been made in medical techniques for regenerating bone. However, bone evaluation techniques generally assess bone quantity as opposed to bone quality. The use of c‐axis crystallite orientation of biological apatite (BAp) as a bone quality index has recently generated great interest. BAp demonstrates strong crystallographic anisotropy, and preferential alignment of BAp in each bone varies depending on the shape and stress conditions in vivo. In the mandible, complicated bone shape and stress conditions in vivo might be associated with both bone quantity and quality. In this study, we aimed to elucidate changes in the bone microstructure in the mandible using crystallographic orientation of BAp as a bone quality index. Using Crj : CD (SD) IGS female rats, we observed changes in the dentulous mandible during bone growth. Measuring points on the mandible were determined based on its positional relationship with the teeth. For analysis of bone quantity, the area and bone mineral density of cortical bone were evaluated using peripheral quantitative computed tomography (pQCT), while the orientation of the BAp c‐axis, as analyzed by a micro‐beam X‐ray diffraction system, was used to assess bone quality. The results of both bone quantity and quality assessments indicated that changes during bone growth varied depending on the presence of teeth. We concluded that the microstructure (especially the texture) of BAp crystallite changes in correlation with variations in stress distribution in vivo resulting from changes in chewing conditions designed to optimize the dynamic chewing function.
Incidence and Characteristics of Ventricular Fibrillation in Bystander‐witnessed Out‐of‐hospital Cardiac Arrest with Cardiac Etiology in the City of Sendai, Japan
Authors: Watanabe Jun , Kanazawa Masaharu , Yagi Tetsuo , Odakura Hironori , Kameyama Motonobu , Sakurai Katsuhiko , Hiramoto Tetsuya , Uenohara Hiroshi , Endo Tomoyuki , Koseki Yoshito , Shinozaki Tsuyoshi , Shiba Nobuyuki , Karibe Akihiko , Sakuma Masahito , Fukuda Koji , Kagaya Yutaka , Numakura Katsunori , Yamaki Masayuki , Shinozawa Yotaro , Shirato Kunio 
Published: August 2012

Ventricular fibrillation (VF) in out‐of‐hospital cardiac arrest (OHCA) is a main target for resuscitation.Methods and results: We analyzed Utstein‐style data in Sendai City (population 1,020,000), Japan from January 2002 to March 2004. The incidence of OHCA overall was 62.3/100,000/year. The incidence of the bystander‐witnessed VF was 2.5/100,000/year. In younger patients (20–65 years of age), the percentage of VF was 52% when cardiac origin was presumed by bystander witness, and ECG was recorded within 10 minutes from the collapse. In older patients (over 65 years of age), however, the percentage of VF was 21% when they were bystander‐witnessed, and ECG was recorded within 10 minutes from the collapse. No VF was reported when the ECG was recorded more than 15 minutes after the collapse. The thirty‐day survival rate was 21% in the bystander‐witnessed VF cases with cardiac etiology, but 0% in the non‐VF cases. The bystander CPR was significantly associated with improved 30‐day survival rate. Conclusion: Younger age, male gender, and shorter collapse‐to‐ECG time are significantly associated with the appearance of VF in bystander‐witnessed OHCA with cardiac etiology. Bystander CPR was significantly associated with the improvement in prognosis of those VF patients.
Modification of Electrophysiological Properties of Pulmonary Veins and Adjacent Left Atrial Tissue by Radiofrequency Thermal Balloon Circumferential Ablation around the Pulmonary Vein Ostia: Correlation with Non‐recurrence of Atrial Fibrillation
Authors: Sohara Hiroshi , Satake Shutaro , Tanaka Kazushi , Saito Shigeru , Domae Hiroshi , Takahashi Saeko , Taketani Yoshio , Shiono Takaaki , Miyashita Yusuke , Tanaka Shinji , Watanabe Yoshio , Ueno Hideki , Suma Hisayoshi , Murakami Masato , Ishigaki Sugako , Takasu Nobuyuki , Yamaguchi Yoshio , Takekoshi Noboru 
Published: August 2012

Objective: The purpose of this study was to provide detailed information as to the modification of electrophysiological properties of pulmonary veins (PVs) and adjacent left atrial (LA) tissue caused by circumferential ablation (ABL) of superior and inferior pulmonary vein ostia using a novel radio‐frequency balloon catheter (RBC), and to correlate such changes with recurrence or non‐recurrence of atrial fibrillation (AF).Background: Although isolation of the triggering foci in PVs using conventional ABL techniques is useful in the treatment of AF, ABL of PVs per se is time consuming and tends to be associated with high AF recurrence rate.Methods and Results: Forty‐nine patients with AF refractory to antiarrhythmic medication underwent circumferential ablation of PV‐LA junction by RBC. Post ablation changes in electrophysiological properties around the PV ostia were studied with a basket catheter and were correlated with AF recurrence in 34 (1st study) early cases (ablating 68 superior PVs) and 15 (2nd study) later cases (53 superior and inferior PVs). Total elimination of PV potentials or PV‐LA dissociation was achieved in 93% (63/68 PVs) and 93% (49/53 PVs), respectively. During mean follow‐up periods of 24.1 ± 4.1 and 13.3 ± 1.7 months, AF recurred in 38% (13/34 cases) and 13% (2/15) in early and later groups, respectively, but no case developed severe PV stenosis. Although the amplitude of PV and periostial LA potentials were decreased (p < 0.0001) in all patients, the remaining PV potentials in 34 non‐recurrence cases were definitely smaller than those in 15 recurrence cases (p <0.0001). When a cut‐off level of less than 0.4 mV in receiver operating characteristic curves was used, its negative predictive value for non‐recurrence of AF was 93% and specificity was 95%.Conclusions: Circumferential ablation around the PV ostia using the RBC is effective in the treatment of AF even in a single session, and AF recurrence may well be predicted by precisely measuring the amplitude of remaining PV‐LA potentials.
Acute Effects on Signal‐Averaged Electrogram Parameters and Suppressing Premature Ventricular Contractions in Single or Combined Use of Class I Antiarrhythmic Drugs
Authors: Kamei Shin‐ichiro , Katoh Takao , Ohara Toshihiko , Kanemura Masafumi , Kuroki Shin‐ichi , Takano Teruo 
Published: August 2012

AbstractDisopyramide (DP), mexiletine (MX), and flecainide (FL) are class I antiarrhythmic drugs. However, these drugs exert different effects on the electrocardiogram (ECG) based on their unique actions on cardiac myocytes. The electrocardiographic changes during combination therapy with these drugs are not well understood. The purpose of the present study was to evaluate acute morphologic changes in the ECG based on signal‐averaged high resolution ECG (SAECG) after administration of the drugs in relation to their antiarrhythmic eficacy and safety. Twenty‐one patients with frequent and stable premature ventricular contractions (PVC) were studied. Changes in the filtered QRS duration (f‐QRS) and the root mean square voltage of the last 40 msec of the QRS complex (RMS40) were evaluated. Suppression of PVCs was achieved soon after intravenous administration of the drugs (63% for DP, 43% for MX, 86% for FL and 100% for DP+MX). Although DP and FL significantly prolonged f‐QRS, MX had little effect on f‐QRS. DP+MX also prolonged f‐QRS, but the degree of prolongation was moderate. RMS40 was significantly decreased by DP and FL, but not by MX. DP+MX also decreased RMS40, but the decrease was less than for DP alone. Late potentials were observed after administration in 56% of patients with DP, 0% with MX, 67% with FL and 0% with DP+MX. No adverse events were reported during the study.In summary, the class I antiarrhythmic drugs exerted different acute effects on SAECG parameters. The combination of DP and MX increased the eficacy on suppressing PVC without excess additive changes on SAECG parameters. We conclude that combination therapy with DP and MX is eficacious and safe in patients with PVC and the analysis of SAECG during antiarrhythmic therapy is clinically important.
Bone Marrow Cell Transplantation into the Heart is Not a Crucial Factor of Ventricular Fibrillation in a Rat Doxorubicin‐Induced Cardiomyopathy Model
Authors: Hamamoto Masaki , Tomita Shinji , Inagaki Masashi , Yutani Chikao , Yamashiro Seiji , Sunagawa Kenji , Nakatani Takeshi , Kitamura Soichiro 
Published: August 2012

AbstractTo verify the susceptibility of ventricle to arrhythmia after bone marrow mononuclear cell transplantation (BMMNCT), we measured ventricular fibrillation threshold (VFT) and monophasic action potential duration (MAYD90). Lewis rats with doxorubicin‐induced cardiomyopathy were divided into 2 groups: transplantation group (D‐TX: BMMNC (1 × 106) given transplants into the apex at 4 weeks after doxorubicin administration) and nontransplantation group (D‐N). Age‐matched normal group (N‐N) was prepared. At 4 weeks after BMMNCT, the following electrophysiologic tests were performed. Experiment 1 (VFT): Two stainless wires were placed at the apex and a single train of 10 square wave stimuli was delivered across T wave. The lowest current developing ventricular fibrillation was determined as VFT. Experiment 2 (MAPD90): The catheter was inserted into the left ventricle and pressed against the endocardium near the apex under regular cycle lengths. Experiment 1: VFT of D‐TX and D‐N were significantly lower than that of N‐N (p < 0.01). There was no difference between D‐TX and D‐N. Experiment 2: MAYD90 of each group was lined up in length D‐N>D‐TX>N‐N at every cycle lengths. There was a significant difference between D‐N and N‐N (p < 0.01), and D‐TX and N‐N (p < 0.05). In this model, BMMNCT did not increase susceptibility to arrthythmia.
When Should We Decide to Perform a Repeat Pulmonary Vein Isolation Procedure in Patients with Atrial Fibrillation?
Authors: Inada Keiichi , Yamane Teiichi , Kanzaki Yasuko , Matsuo Seiichiro , Shibayama Kenri , Miyanaga Satoru , Date Taro , Miyazaki Hidekazu , Sugimoto Kenichi , Mochizuki Seibu 
Published: August 2012

AbstractBackground: It is sometimes difficult to differentiate the transient appearance of atrial fibrillation (AF) after pulmonary vein (PV) isolation from a true recurrence of AF. We attempted to differentiate them by analyzing the time course after the procedure.Methods and Results: 79 patients who underwent PV isolation were divided into two groups (successful: N = 60 and unsuccessful: N = 19) according to the final outcome. Antiarrhythmic drugs were used either temporarily or continuously to treat re‐appearance of AF after the procedure. The transient appearance of AF in the successful group gradually faded, while true AF recurrence in the unsuccessful group consistently increased in line with the follow‐up (F/U) period. The appearance of AF after 3 months predicted a subsequent failure of the procedure with a positive/negative predictive value of 87/90%, respectively.Conclusion: Since the transient appearance of AF decreased and the true recurrence of AF increased in line with the F/U period, we should therefore wait at least 3 months before judging the outcome of PV isolation.
Non‐Pharmacological Management of Neurocardiogenic Syncope
Authors: Abe Haruhiko , Kohno Ritsuko , Sumiyoshi Masataka , Oginosawa Yasushi , Takemasa Hiroko , Tsurugi Takuo , Nagatomo Toshihisa , Otsuji Yutaka 
Published: August 2012

AbstractNeurocardiogenic syncope is a common disorder. It is diagnosed by obtaining a detailed history and performing a head‐up tilt test, with or without drug provocation. Several studies have been performed pertaining to its management. However, no treatment, whether pharmacological or non‐pharmacological, except for counterpressure maneuvers and daily orthostatic tilt training, has been proven effective. Randomized studies of therapies for neurocardiogenic syncope are needed.
Coronary Sinus Activation Pattern in Patients with Atrioventricular Nodal Reentrant Tachycardia
Authors: Yagi Tetsuo , Scheinman Melvin M 
Published: August 2012

Patterns of left atrial (far‐field signals) or coronary sinus (CS) muscle (near‐field) have been defined in CS recordings. The purpose of this study was to define the activation patterns from the coronary sinus in patients with anterior and posterior type of atrioventricular nodal reentrant tachycardia (AVNRT) circuits.Methods and Results: This retrospective study involved a total of 149 patients with 155 episodes of AVNRT which were divided into 3 patterns. In the anterior pattern (123 tachycardias), the atrial deflection from the His bundle electrogram preceded that from the proximal CS electrogram. In the posterior pattern (23 tachycardias), the proximal CS electrogram (CSp) was recorded earlier than the His bundle atrial electrogram. In the left atrial pattern (9 tachycardias), activation of distal CS sites preceded both proximal CS and atrial activation from the His bundle electrogram. A decapolar catheter with a 5‐mm interelectrode distance was used for CS recording. The CS electrograms were analyzed to determine the total signal duration as well as the duration of the initial component. An initial slow wave was defined as a duration exceeding 10 ms. 1) The duration of the initial component in patients with the anterior pattern was longer than in those with the posterior pattern in CSp (7.3 ± 3.1 ms vs. 4.5 ± 2.0 ms), CS7‐8 (7.4 ± 2.9 ms vs. 3.8 ± 1.5 ms), CS5‐6 (7.3 ms ± 3.3 ms vs. 4.4 ms ± 2.5 ms) and CS3‐4 (6.7 ms ± 2.4 ms vs. 4.5 ms ± 2.0 ms) (p < 0.01). 2) Similarly the total electrogram duration in the CS was longer in patients with an anterior compared to a posterior pattern in CSp (38.3 ms ± 10.1 ms vs. 26.8 ± 6.1 ms), CS7‐8 (31.8 ms ±6.6 ms vs. 27.2 ms ± 6.0 ms), CS5‐6 (31.3 ms ± 6.8 ms vs. 26.5 ms ± 4.9 ms), and CS3‐4 (30.0 ms ± 6.3 ms vs. 25.0 ms ± 5.1 ms) (p < 0.01). 3) The percentage of tachycardias showing an initial slow wave followed by rapid activation was higher for anterior pattern patients compared with posterior pattern patients in CSp (62% vs. 13%), CS7‐8 (79% vs. 4%), CS5‐6 (72% vs. 4%), CS3‐4 (54% vs. 9%) and distal CS (47% vs. 0%) (p < 0.01).Conclusions: The pattern of an initial slow wave followed by a rapid wave in the CS was characteristic of an anterior AVNRT circuit and is explained by the initial involvement of far field left atrial components. In contrast, the predominant early rapid waves in the posterior AVNRT circuit are compatible with early CS activation from the right atrium.
Primary Prevention of Sudden Cardiac Death in Patients with Ischemic Heart Disease —Possible Role of the Shock Device in the Asia—
Authors: Kurita Takashi 
Published: August 2012

Recently several randomized trials have demonstrated the excellent role of the implantable cardioverter‐defibrillator (ICD) in improving the mortality rate in patients with a high risk of sudden cardiac death regardless of its purpose or the underlying heart disease. However, because such large clinical trials have only been carried out in Western countries, those results may not be fully applicable to Japanese or other Asian patient populations.According to a Japanese study, the total mortality in the patients was approximately 20% during 5 years of follow up, which was comparable to, or slightly better than that in the MADIT‐II patients with ICDs. In such a patient population with an excellent prognosis, the MADIT‐II criteria can not easily be applied. Therefore, we should strengthen the criteria, i.e. from an EF ≤ 35% to 25% or adopt the usage of additional examinations (e.g. signal averaging ECG, micro‐volt T wave alternance or EPS).
Suppression of Ventricular Tachycardia Associated with Cardiac Sarcoidosis by Steroid Therapy
Authors: Miyaji Kotaro , Suzuki Makoto , Mizukami Akira , Iwatsuka Ryota , Kumasaka Reon , Ichihara Naoaki , Nagahori Wataru , Oono Masakazu , Arakawa Tetsuo , Masaki Nobuyuki , Matsumura Akihiko , Hashimoto Yuji 
Published: August 2012

In patients with cardiac sarcoidosis, ventricular tachycardia (VT) is observed in some cases. However, effective therapies for the VT are still unknown.Case: A 50‐year old female with cardiac sarcoidosis underwent DDD pacemaker implantation for a high degree atrioventricular block with symptoms of faintness and shortness of breath. One month after the surgery, she was admitted for frequent episodes of non‐sustained VT. In the electrophysiologic study (EPS), sustained monomorphic VT and ventricular fibrillation were induced; therefore pacemaker was replaced with implantable cardioverter‐defibrillator (ICD). Amiodarone was started orally but it couldn't suppress frequent VT episodes, and frequent ICD shocks were delivered. When the oral steroid therapy was initiated for the cardiac sarcoidosis, it not only suppressed the frequent VT but also improved the atrioventricular nodal dysfunction.In conclusion, steroid therapy might be an option to consider in cardiac sarcoidosis with refractory VT.
The Prognosis of Patients who Received Automated External Defibrillator Treatment in Hospital
Authors: Kato Isao , Iwa Toru , Suzuki Yasushi , Ito Takayuki 
Published: August 2012

Introduction: Unlike cardiac arrest occurring out‐of‐hospital, the safety and efficacy of automated external defibrillators (AED) in the hospital has not been assessed. This study examined the conditions of AED use in hospital and the prognosis of these patients.Methods and Results: We examined the condition and prognosis of 32 patients who were given AED treatment while they were in an unconscious state in the hospital, between May 2004 and January 2007. During this period, AED was used only for patients, not for visitors or hospital personnel.Ventricular fibrillation (VF) or ventricular tachycardia (VT) was observed in 7 patients, and in the other 25 the initial rhythm of the patients did not require AED. Two patients survived with the help of AED, but it did not deliver shock in two patients with VF and VT. There was no significant difference in vital prognosis due to the presence or absence of shock delivery in the VF or VT patients.Conclusion: The situation of AED use may be different whether it is used in hospital or outof‐hospital. This study suggests that using AED in the hospital may have limited effect when it is used for critically ill patients.