bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplex target capture with double-stranded DNA probes
Authors: Shen Peidong , Wang Wenyi , Chi Aung-Kyaw , Fan Yu , Davis Ronald W , Scharfe Curt 
Published: May 2013

AbstractTarget enrichment technologies utilize single-stranded oligonucleotide probes to capture candidate genomic regions from a DNA sample before sequencing. We describe target capture using double-stranded probes, which consist of single-stranded, complementary long padlock probes (cLPPs), each selectively capturing one strand of a genomic target through circularization. Using two probes per target increases sensitivity for variant detection and cLPPs are easily produced by PCR at low cost. Additionally, we introduce an approach for generating capture libraries with uniformly randomized template orientations. This facilitates bidirectional sequencing of both the sense and antisense template strands during one paired-end read, which maximizes target coverage.
DAP12 impacts trafficking and surface stability of killer immunoglobulin-like receptors on natural killer cells
Authors: Mulrooney Tiernan J , Posch Phillip E , Hurley Carolyn Katovich 
Published: May 2013

ABSTRACTKIR aid in the regulation of NK cell activity. In this study, the effect of the interaction between the KIR2DS and their adapter, DAP12, was investigated beyond the previously defined signaling function. Flow cytometry analysis showed enhanced KIR2DS surface expression on NKL cells when cotransfected with DAP12. Conversely, KIR2DS4 surface expression on primary cells was decreased when the cells were treated with DAP12-specific siRNA. Treatment of the KIR2DS and DAP12-transfected cells with CHX or BFA repressed KIR2DS surface expression, revealing a role for DAP12 in trafficking newly synthesized KIR to the cell surface. Immunoprecipitation of DAP12 revealed an interaction of DAP12 with an immature isoform of KIR2DS, indicating that the interaction likely initiates within the ER. An internalization assay demonstrated a significant impact of DAP12 on KIR2DS surface stability. Confocal microscopy showed that internalized KIR2DS molecules are recruited to lysosomal compartments independent of DAP12 expression. Our results suggest that in vivo conditions that adversely affect DAP12 expression will indirectly reduce surface expression and stability of KIR2DS. These effects could significantly impact ligand recognition and strength of signaling through KIR2DS molecules.
Predictive Gene Signature in MAGE-A3 Antigen-Specific Cancer Immunotherapy
Authors: Ulloa-Montoya Fernando , Louahed Jamila , Dizier Benjamin , Gruselle Olivier , Spiessens Bart , Lehmann Frédéric F. , Suciu Stefan , Kruit Wim H.J. , Eggermont Alexander M.M. , Vansteenkiste Johan , Brichard Vincent G. 
Published: May 2013

Purpose To detect a pretreatment gene expression signature (GS) predictive of response to MAGE-A3 immunotherapeutic in patients with metastatic melanoma and to investigate its applicability in a different cancer setting (adjuvant therapy of resected early-stage non–small-cell lung cancer [NSCLC]). Patients and Methods Patients were participants in two phase II studies of the recombinant MAGE-A3 antigen combined with an immunostimulant (AS15 or AS02B). mRNA from melanoma biopsies was analyzed by microarray analysis and quantitative polymerase chain reaction. These results were used to identify and cross-validate the GS, which was then applied to the NSCLC data. Results In the patients with melanoma, 84 genes were identified whose expression was potentially associated with clinical benefit. This effect was strongest when the immunostimulant AS15 was included in the immunotherapy (hazard ratio [HR] for overall survival, 0.37; 95% CI, 0.13 to 1.05; P = .06) and was less strong with the other immunostimulant AS02B (HR, 0.84; 95% CI, 0.36 to 1.97; P = .70). The same GS was then used to predict the outcome for patients with resected NSCLC treated with MAGE-A3 plus AS02B; actively treated GS-positive patients showed a favorable disease-free interval compared with placebo-treated GS-positive patients (HR, 0.42; 95% CI, 0.17 to 1.03; P = .06), whereas among GS-negative patients, no such difference was found (HR, 1.17; 95% CI, 0.59 to 2.31; P = .65). The genes identified were mainly immune related, involving interferon gamma pathways and specific chemokines, suggesting that their pretreatment expression influences the tumor's immune microenvironment and the patient's clinical response. Conclusion An 84-gene GS associated with clinical response for MAGE-A3 immunotherapeutic was identified in metastatic melanoma and confirmed in resected NSCLC.
Resolution ofClostridium difficile–Associated Diarrhea in Patients With Cancer Treated With Fidaxomicin or Vancomycin
Authors: Cornely Oliver A. , Miller Mark A. , Fantin Bruno , Mullane Kathleen , Kean Yin , Gorbach Sherwood 
Published: May 2013

PurposePatients with cancer are at increased risk for Clostridium difficile–associated diarrhea (CDAD). Little is known about treatment response.Patients and MethodsTwo double-blind trials randomly allocated 1,105 patients with CDAD to fidaxomicin or vancomycin treatment (modified intent-to-treat [mITT]), and 183 of these had cancer. Univariate and multivariate post hoc analyses compared effects of treatment and patient characteristics on cure, recurrence, and sustained response after 4 weeks. Time to resolution of diarrhea (TTROD) was also evaluated.ResultsPatients with cancer had a lower cure rate and longer TTROD than patients without cancer. Recurrence rates were similar. Cure was more likely with fidaxomicin than vancomycin (odds ratio [OR] 2.0; P = .065), recurrence was less likely (OR = 0.37; P = .018), and sustained response more frequent (OR = 2.56; P = .003). Under vancomycin, median TTROD was longer in patients with cancer than in those without (123 v 58 hours; log-rank P < .001). With fidaxomicin, median TTROD was not significantly affected by presence of cancer (74 v 54 hours; log-rank P = .145). In the full mITT population, age, hypoalbuminemia, and cancer were inversely associated with clinical cure by multivariate analysis. Study treatment with vancomycin was a significant predictor of recurrence (P < .001). Within the cancer population, low albumin was negatively and fidaxomicin was positively associated with improved cure.ConclusionFor patients with cancer, fidaxomicin treatment was superior to vancomycin, resulting in higher cure and sustained response rates, shorter TTROD, and fewer recurrences.
Prospective Investigation of Body Mass Index, Colorectal Adenoma, and Colorectal Cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
Authors: Kitahara Cari M. , Berndt Sonja I. , de González Amy Berrington , Coleman Helen G. , Schoen Robert E. , Hayes Richard B. , Huang Wen-Yi 
Published: May 2013

Purpose Obesity has consistently been linked to an increased risk of colorectal cancer, particularly among men. Whether body mass index (BMI) differentially influences the risk across the stages of colorectal cancer development remains unclear. We evaluated the associations of BMI with colorectal adenoma incidence, adenoma recurrence, and cancer in the context of a large screening trial, in which cases and controls had an equal chance for disease detection. Methods We prospectively evaluated the association between baseline BMI and the risk of incident distal adenoma (1,213 cases), recurrent adenoma (752 cases), and incident colorectal cancer (966 cases) among men and women, ages 55 to 74 years, randomly assigned to receive flexible sigmoidoscopy screening as part of the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. We calculated odds ratios (ORs) and 95% CIs for adenoma incidence and recurrence, and hazard ratios (HRs) and 95% CIs for colorectal cancer incidence, using multivariable-adjusted models. Results Compared with normal-weight men (18.5 to 24.9 kg/m2), obese men (≥ 30 kg/m2) had significantly higher risk of incident adenoma (OR, 1.32; 95% CI, 1.06 to 1.65) and colorectal cancer (HR, 1.48; 95% CI, 1.16 to 1.89) and a borderline increased risk of recurrent adenoma (OR, 1.50; 95% CI, 0.98 to 2.30). No associations were observed for either adenoma or cancer in women. Conclusion Data from this large prospective study suggest that obesity is important throughout the natural history of colorectal cancer, at least in men, and colorectal cancer prevention efforts should encourage the achievement and maintenance of a healthy body weight in addition to regular screenings.
Forty years of olfactory navigation in birds
Authors: Gagliardo Anna 
Published: May 2013

Summary
Forty years ago, Papi and colleagues discovered that anosmic pigeons cannot find their way home when released at unfamiliar locations. They explained this phenomenon by developing the olfactory navigation hypothesis: pigeons at the home loft learn the odours carried by the winds in association with wind direction; once at the release site, they determine the direction of displacement on the basis of the odours perceived locally and orient homeward. In addition to the old classical experiments, new GPS tracking data and observations on the activation of the olfactory system in displaced pigeons have provided further evidence for the specific role of olfactory cues in pigeon navigation. Although it is not known which odours the birds might rely on for navigation, it has been shown that volatile organic compounds in the atmosphere are distributed as fairly stable gradients to allow environmental odour-based navigation. The investigation of the potential role of olfactory cues for navigation in wild birds is still at an early stage; however, the evidence collected so far suggests that olfactory navigation might be a widespread mechanism in avian species.
Limits to sustained energy intake. XVII. Lactation performance in MF1 mice is not programmed by fetal number during pregnancy
Authors: Duah Osei A. , Monney Kweku A. , Hambly Catherine , Król Elzbieta , Speakman John R. 
Published: May 2013

SUMMARYSeveral studies have suggested that lactation performance may be programmed by the number of fetuses during pregnancy, whereas other studies indicate that processes during lactation are more important. As gestation litter size and litter size in lactation are usually strongly correlated, separating the roles of pregnancy and lactation in lactation performance is difficult. To break this link, we experimentally manipulated litter size of MF1 mice to five or 16 pups per litter by cross-fostering. Litter size and mass at birth were recorded on day 1 of lactation prior to litter size manipulation. Maternal body mass and food intake, litter size and litter mass were measured daily throughout. After weaning, the potential differential utilisation of body tissues of the mothers was investigated. Relationships between maternal mass and food intake, including asymptotic daily food intake at peak lactation, offspring traits and other maternal parameters suggested that the number of fetuses the females had carried during pregnancy had no effect on lactation performance. Litter mass increases depended only on maternal food intake, which was highly variable between individuals, but was independent of fetal litter size. The sizes of key organs and tissues like the liver and alimentary tract were not related to maximal food intake at peak lactation or to fetal litter size, but the masses of the pelage, mammary glands and retroperitoneal fat pad were. These data suggest that while growth of the mammary glands and associated structures may be initiated in gestation, and vary in relation to the number of placentas, the ultimate sizes and activities of the tissues depends primarily on factors during lactation.
Iron(III)-catalyzed Highly Efficient, One-pot Synthesis of Triazolo[1,2-a]indazoletriones and Spirotriazolo[1,2-a]indazoletetraones
Authors: Reddy B V Subba , Umadevi N , Narasimhulu G , Yadav J S 
Published: May 2013

Abstract
Three-component coupling (3CC) of aldehyde, 1,3-dione, and 4-phenylurazole has been achieved in the presence of 10 mol % anhydrous FeCl3 in acetonitrile under reflux conditions to afford the corresponding triazolo[1,2-a]indazoletriones in excellent yields while the coupling of isatins with 4-phenylurazole and 1,3-diones gave the spirotriazolo[1,2-a]indazoletetraones under similar conditions.
Mussel-inspired Engineering of an Anodized Aluminum Oxide Membrane
Authors: Kim So Yeon , Lee Mi Yeon , Lee Jung Yup , Park Young Ho , Kim Hyun Gu , Jeong Chan Jin , Mosaiab Tamim , Park Byoungnam , Park Sung Young , In Insik 
Published: May 2013

Abstract
A thermosensitive membrane was prepared through mussel-inspired engineering of anodized aluminum oxide (AAO) membrane. Incorporation of catechol-functionalized copolymer having both 2-(dimethylamino)ethyl methacrylate (DMAEMA) and N-isopropylacrylamide (NIPAM) on AAO membrane provided stimuli-response to both temperature and pH. From the decrease of both water droplet contact angle and buffer solution transport rate above lower critical solution temperature (LCST) of copolymer, a “wettability”-controlled regulation mechanism of mass-transfer rate through engineered AAO membrane was proposed.
Preparation of Mesoporous Silica Monoliths Doped with Titanium Clusters
Authors: Nogawa Takashi , Matsushita Sachiko , Isobe Toshihiro , Nakajima Akira 
Published: May 2013

Abstract
Hierarchical nanoporous structures were fabricated from mesoporous silica and organic monolith with the aim of fabricating a high-efficiency catalyst carrier. The hierarchical structures were modified with photocatalytic titanium clusters. The hierarchical structures showed higher photodecomposition rate of isopropyl alcohol than mesoporous silica structures or monolith structure did, even though the surface area of the hierarchical structure was the smallest. In addition, the CH4 generation from CO2 photoreduction on the hierarchical mesoporous structure was also confirmed. These results suggest the possibilities of highly efficient mass-transport property and the ease of chemical modification of our hierarchical structure.
Conjugate Addition of α-Sulfur-substituted Alkylstannanes to α,β-Unsaturated Ketones Mediated by a Copper(II) Triflate and Chlorotrimethylsilane Combination
Authors: Kagoshima Hirotaka , Yahagi Takeshi 
Published: May 2013

Abstract
The copper(II) triflate and chlorotrimethylsilane combination was found to be effective for the conjugate addition of α-sulfur-substituted alkylstannanes to α,β-unsaturated ketones. Thus, the corresponding γ-sulfur-substituted ketones were obtained in good yields.
Prostitution use has non sexual functions - case report of a depressed psychiatric out-patient
Authors: Gysin Fátima , Gysin François 
Published: May 2013

Case: A shy, depressed 30 year old male discussed his frequent ego-syntonic indoor prostitution consumption in small peer groups. Several distinctive non-sexual functions of this paid sex habit were identified.Design and method: The patient had 40 hourly psychiatric sessions in the private practice setting over 14 months. The Arizona Sexual Experience Scale was applied to compare the subjective appraisal of both paid sex and sex in a relationship. The informal Social Atom elucidates social preferences and the Operationalized Psychodynamic Diagnostic-procedure was applied to describe a dominant relationship pattern.Results: The paid sex consumption functioned as a proud male life style choice to reinforce the patients fragile identity. The effect on self esteem was a release similar to his favorite past-time of kick-boxing. With paid sex asserted as a group ritual, it was practiced even with frequent erectile dysfunction and when sex with a stable romantic partner was more enjoyable and satisfying. The therapeutic attitude of the female psychiatrist, with her own ethical values, is put in to context with two opposing theories about prostitution: the ‘Sex-Work-model’ and the ‘Oppression-model’. The therapist’s reaction to the patients’ information was seen as a starting point to understanding the intrapsychic function of paid sex as a coping mechanism against depressive feelings.Conclusions: Exploring and understanding prostitution consumption patterns in young men can benefit the treatment of psychiatric disorders in the private practice setting. It is the psychiatrists task to investigate the patients hidden motives behind paid sex use to help patients achieve a greater inner and relational freedom.
Tremors and Agitation Following Low-Dose Intravenous Hydromorphone Administration in a Patient with Kidney Dysfunction
Authors: Gagnon David J , Jwo Kevin 
Published: May 2013

OBJECTIVE To report a case of tremors and agitation associated with the administration of low doses of intravenous hydromorphone in a patient with acute kidney injury in the setting of chronic kidney disease. CASE SUMMARY A 91-year-old man was admitted for a left intertrochanteric hip fracture. On hospital days 1 and 2, the patient received hydromorphone 1 mg intravenously for pain. By hospital day 3, the patient had received a total of 3.5 mg of hydromorphone. He then became tremulous and agitated, which worsened as the dose was increased. During hospital day 4, the hydromorphone dosage was increased to 0.5 mg intravenously every 4 hours for what was perceived to be inadequate pain control, manifesting as tremors and agitation. On hospital day 5, hydromorphone, now considered a potential cause of the tremors and agitation, was discontinued. The total amount of hydromorphone administered was 8 mg over 5 days. By hospital day 6, the tremors and agitation had decreased in severity and had resolved by hospital day 7. DISCUSSION Previous reports suggest that hydromorphone-induced neurotoxicity is more likely to occur following high doses over extended periods, especially in patients with kidney dysfunction. There is a paucity of evidence suggesting that neurotoxicity can occur following low doses for short periods of time. Morphine-3-glucuronide has neuroexcitatory properties including myoclonus, allodynia, agitation, or tremors. Structural similarities between morphine-3-glucuronide and hydromorphone-3-glucuronide suggest that neuroexcitatory effects with hydromorphone are feasible. The Naranjo probability scale indicated that hydromorphone-induced tremors were possible. Our case is distinct because the patient received low doses of hydromorphone over a short period. CONCLUSIONS Neurotoxicity can occur in patients with kidney dysfunction while they receive low doses of intravenous hydromorphone over short periods. Diligent monitoring and reporting of such effects are necessary to better understand risk factors and a mechanism.
A Study of Total Focusing Method for Ultrasonic Nondestructive Testing
Authors: Tseng Po-Yen , Chang Young-Fo , Lin Chao-Ming , Nien Wei-Jen , Chang Chih-Hsiung , Huang Chih-Chung 
Published: May 2013

Abstract
Commercial ultrasonic phased array (PA) systems can perform multiple inspections in real-time using array transducers. This approach involves firing array elements in a particular sequence to produce high-resolution images at a high frame rate with excellent signal-to-noise ratio (SNR). Unfortunately, PA systems are complex and expensive. This study proposes a simpler, cheaper, non-real-time ultrasonic array system incorporating the total focusing method (TFM), and the performance of the proposed system is compared with that of a commercial PA system. Studying results shows that both systems have similar apparent axial resolution, but the apparent lateral resolution of the proposed system is far better than the L-scan but falls short in S-scan of a commercial PA system under a quiet testing environment. Moreover, the array performance indicator (API) values of TFM are lower than those of S-scan and L-scan, and the TFM has the highest SNR than the other methods. These results show that the performance of TFM array system is better than the commercial PA system, except for time consumption when switching the channels. Automatically switching the source and receiver channels could further the development of inexpensive, high-performance TFM arrays for ultrasonic nondestructive testing (NDT). It is anticipated that rapid advancements in electronic components, computer science, and digital signal processing will lead to improvements in near-real-time TFM array systems.
Two-Stage Group Acceptance Sampling Plan for Burr Type X Percentiles
Authors: Aslam Muhammad , Azam Muhammad , Lio Y. L. , Jun Chi-Hyuck 
Published: May 2013

Abstract
A two-stage group acceptance sampling plan for the percentile of lifetimes which have the Burr type X distribution is discussed under the time truncated life test. The sampling plan parameters for the two-stage sampling plan are determined such that the producer's risk and consumer's risk are satisfied simultaneously for the given tester size and experiment time. The advantage of the proposed two-stage sampling plan over the single-stage sampling plan is also discussed. Useful tables are given for practical purpose.
Application of the Stress-Energy Method for Generating Corrugated Board Cushion Curves
Authors: Daum Matthew , Darby Duncan , Batt Gregory , Campbell Lisi 
Published: May 2013

Abstract
Cushion curves are an important tool used for designing foam cushions, but no commercially available curves exist for corrugated board, despite its growing popularity as a cushioning material. This paper summarizes the theory and recent work applying the stress-energy method and different curve fit models to corrugated board for generating cushion curves. This paper also compares stress-energy predicted deceleration values to actual ASTM D1596 deceleration values as a method of determining whether the stress-energy method is a viable alternative for generating cushion curves for corrugated board. The results of this study suggest that the stress-energy method should not yet be recommended for generating cushion curves for corrugated board.
Regelung von doppeltspeisenden Drehstromgeneratoren für den energieeffizienten Betrieb
Authors: Hofmann Wilfried , Rabelo Baldino 
Published: May 2013

Zusammenfassung
Der Beitrag behandelt die Regelung von Windenergieanlagen, die mit doppeltspeisenden Asynchrongenerator ausgestattet sind. Die eingesetzte Regelstruktur ist so gestaltet, dass sie in der Lage ist moderne Anforderungen des Netzbetriebs zu erfüllen und diese mit einem energieeffizienten Betrieb des Energiewandlers zu verbinden. Diskutiert werden ausgehend von einer vereinfachten Regelstrecke die kaskadierten Regelkreise, die Reglerauslegung und die erreichten experimentellen Ergebnisse.
A Genetic Screen for High Copy Number Suppressors of the Synthetic Lethality Betweenelg1Δandsrs2Δin Yeast
Authors: Gazy Inbal , Liefshitz Batia , Bronstein Alex , Parnas Oren , Atias Nir , Sharan Roded , Kupiec Martin 
Published: May 2013

AbstractElg1 and Srs2 are two proteins involved in maintaining genome stability in yeast. After DNA damage, the homotrimeric clamp PCNA, which provides stability and processivity to DNA polymerases and serves as a docking platform for DNA repair enzymes, undergoes modification by the ubiquitin-like molecule SUMO. PCNA SUMOylation helps recruit Srs2 and Elg1 to the replication fork. In the absence of Elg1, both SUMOylated PCNA and Srs2 accumulate at the chromatin fraction, indicating that Elg1 is required for removing SUMOylated PCNA and Srs2 from DNA. Despite this interaction, which suggests that the two proteins work together, double mutants elg1Δ srs2Δ have severely impaired growth as haploids and exhibit synergistic sensitivity to DNA damage and a synergistic increase in gene conversion. In addition, diploid elg1Δ srs2Δ double mutants are dead, which implies that an essential function in the cell requires at least one of the two gene products for survival. To gain information about this essential function, we have carried out a high copy number suppressor screen to search for genes that, when overexpressed, suppress the synthetic lethality between elg1Δ and srs2Δ. We report the identification of 36 such genes, which are enriched for functions related to DNA- and chromatin-binding, chromatin packaging and modification, and mRNA export from the nucleus.