bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Infection of greater white-toothed Shrews (Crocidura russula) with Borna disease virus 1: Insights into Viral Spread and Shedding
Authors: Nobach Daniel , Raeder Leif , Müller Jana , Herzog Sybille , Eickmann Markus , Herden Christiane 
Published: July 2024

Numbers of human encephalitis cases caused by infection with Borna disease virus 1 (BoDV1) increase continuously in endemic areas. The reservoir host of BoDV1 is the bicoloured white-toothed shrew, albeit few naturally infected individuals of other shrew species have been detected. To establish a reliable experimental reservoir model, 15 greater white-toothed shrews were infected with a shrew-derived BoDV1 isolate by different inoculation routes (intracerebral, intranasal, oral, subcutaneous, intraperitoneal) and monitored up to 41 days. Except for the oral route all other animals (12/15) were successfully infected, and the majority of them displayed temporary reduced feed intake and loss of body weight but no inflammatory lesions. Infectious virus was isolated from 11/12 infected animals. Viral RNA was demonstrated by RT-qPCR in the central nervous system (CNS) and the majority of organs.Immunohistochemistry demonstrated BoDV1 antigen in neurons and astrocytes in the CNS and peripheral nerves. High viral loads in the CNS and the spinal cord points towards spread from periphery to the CNS to enhance viral replication, and subsequent centrifugal spread to organs capable of secretion and excretions. In general, successful experimental BoDV1 infection of shrews proves their usefulness as animal model, enabling further studies on maintenance, transmission, pathogenesis, and risk assessment for human spill-over infections.
The Extracellular Matrix Limits Nanoparticle Diffusion and Cellular Uptake in a Tissue-Specific Manner
Authors: Cahn Devorah , Stern Alexa , Buckenmeyer Michael , Wolf Matthew , Duncan Gregg A. 
Published: July 2024

AbstractOverexpression and remodeling of the extracellular matrix (ECM) in cancer and other diseases may significantly reduce the ability of nanoparticles to reach target sites, preventing effective delivery of therapeutic cargo. Here, we evaluate how tissue-specific properties of the ECM affect nanoparticle diffusion using fluorescence video microscopy and cellular uptake via flow cytometry. In addition, we determined how PEGylation chain size and branching influence the ability of nanoparticles to overcome the ECM barrier from different tissues. We found that purified collagen, in the absence of other ECM proteins and polysaccharides, presented a greater barrier to nanoparticle diffusion as compared to decellularized ECM from the liver, lung, and small intestine submucosa. Nanoparticles with dense PEG coatings achieved up to ∼2000-fold enhancements in diffusion rate and cellular uptake up to ∼5-fold greater than non-PEGylated nanoparticles in the presence of ECM. We also found nanoparticle mobility in the ECM varied significantly between tissue types and the optimal nanoparticle PEGylation strategy to enhance ECM penetration was strongly dependent on ECM concentration. Overall, our data supports the use of low molecular weight PEG coatings which provides an optimal balance of nanoparticle penetration through ECM and uptake in target cells. However, tissue-specific enhancements in ECM penetration and cellular uptake were observed for nanoparticles bearing a branched PEG coating. These studies provide new insight into tissue specific ECM barrier functions which can facilitate the design of nanoparticles that effectively transport through target tissues, improving their therapeutic efficacy.
Dual-color μ-LEDs integrated neural interface for multi-control optogenetic electrophysiology
Authors: Ko Eunah , Vöröslakos Mihály , Buzsáki György , Yoon Euisik 
Published: July 2024

AbstractOver recent decades, optogenetics has become a pivotal technique for elucidating the functionality of neuronal circuits in living organisms. By genetically modifying specific cells within targeted tissues to respond to particular optical stimuli, researchers can achieve precise activation or inhibition of these cells. This capability enables detailed investigations of neural circuitry with unprecedented accuracy. However, there is a rising need for hardware that supports bidirectional control in conjunction with electrophysiological recording. A significant challenge in this domain is the compact integration of dual light sources and a recording system. This study addresses this challenge through the development of a novel microfabrication and assembly technique for embedding dual-color micro-LEDs and recording electrodes into a Michigan-type neural probe structure, designated as DuoLite (Dual-color micro-LEDs Integrated Neural-Interface Optoelectrode for Multi-Control Optogenetic Electrophysiology). We present two device variants: (a) a small-group and (b) a large-group cell-targeted design, each incorporating micro-LEDs with a minimal area of <100 μm2for both red and blue light. The design and assembly techniques for integrating all three components within a shank width of <100 μm are thoroughly detailed, and the functionality of the devices is validated through in vivo experiments.
Nonlinear sound-sheet microscopy: imaging opaque organs at the capillary and cellular scale
Authors: Heiles Baptiste , Nelissen Flora , Terwiel Dion , Park Byung Min , Munoz Ibarra Eleonora , Matalliotakis Agisilaos , Waasdorp Rick , Ara Tarannum , Barturen-Larrea Pierina , Duan Mengtong , Shapiro Mikhail G. , Gazzola Valeria , Maresca David 
Published: July 2024

AbstractLight-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescent reporters limit this method to studying thin or translucent specimens. Here we show that non-diffractive ultrasonic beams used in conjunction with a cross-amplitude modulation sequence and nonlinear acoustic reporters enable fast and volumetric imaging of targeted biological functions. We report volumetric imaging of tumor gene expression at the cm3scale using genetically encoded gas vesicles, and localization microscopy of currently uncharted cerebral capillary networks using intravascular microbubble contrast agents. Nonlinear sound-sheet microscopy provides a ∼64x acceleration in imaging speed, ∼35x increase in imaged volume and ∼4x increase in classical imaging resolution compared to the state-of-the-art in biomolecular ultrasound.
Organizational legitimacy and cross-border acquisition: emerging market multinationals
Authors: Yang Buyun , Zhang Shuman , Wu Bo 
Published: July 2024

PurposeEmerging market multinationals often face a variety of legitimacy challenges as they engage in cross-border acquisitions in developed countries, which requires an assortment of legitimacy strategies best aligned with the legitimacy challenges they face. This study advocates for a configurational perspective that examines how different configurations of legitimacy challenges, organizational characteristics, and legitimacy strategies influence the likelihood of deal completion in cross-border acquisitions by emerging market multinational enterprises (EMNEs).Design/methodology/approachBased on 328 cross-border acquisition cases by Chinese firms, this study adopts the fuzzy-set qualitative comparative analysis to examine the combined effects of institutional distance, political affinity, equity sought, architecture design, sensitive·industry and state-owned and enterprise (SOE) on cross-border acquisition completion.FindingsThis study identifies six pathways with different configurations for deal completion, suggesting that a deal's overall legitimacy falls at the intersection of the country-level institution and the firm-level characters and strategy evaluations.Originality/valueThis study investigates how nested legitimacy influences cross-border acquisition completion by offering a holistic and configurational understanding of the deal completion of cross-border acquisitions by EMNEs and yields useful insights for future research on cross-border acquisition completion and legitimacy.
A spatial scientometric analysis of urban planning publications from 2010–2020: geographic disparities in urban planning knowledge production
Authors: Okour Yasmein , Alrayyan Kawthar , Zidan Roa’a 
Published: July 2024

PurposeThis paper analyzes and illustrates the spatial distribution of publications in international urban planning journals from 2010 to 2020.Design/methodology/approachThe study employs the Biblioshiny bibliometrix package in R to analyze 44,123 articles from 95 international planning journals. To conduct the spatial scientometric analysis, we adopted the United Nations’ geoscheme focusing on three geographical scales: country, subregion, and region. Collaboration patterns at the country and subregional levels were examined using the VOSviewer tool.FindingsThe study found evidence of a spatial polarization of urban planning scholarly knowledge production. Scholars based in the United States and the United Kingdom consistently published at higher rates than any other country in our data set. The region producing the largest number of publications was Europe, consisting of 39.92% of the total publication count. However, urban planning scholars from the Global South authored only 20.96% of planning publications from 2010–2020. Centralization of planning research is also evident within each region and subregion. As such, both the Global North and the Global South should not be framed as homogenous entities and spatial patterns of knowledge production should not be generalized. The analysis also established the emerging role of Northern America as a major collaborator in inter-country and inter-subregional research collaborations. Co-authorship patterns indicate low intra-regional collaboration in planning research, except for Europe.Originality/valueThis article argues that a culture of exclusivity may be occurring in urban planning publication production. By highlighting the spatial disparities in knowledge production, we emphasize the need to examine the structural and institutional barriers that exclude urban planning knowledge emerging from the peripheries in international planning journals.
Towards green entrepreneurship: The role of financial openness in driving energy innovation in top carbon emitter economies
Authors: Bo Li , Zhongping Shen , Chao Tan , Bo Tan Hao , Zhen Liu , Naqvi Syed Muhammad Muddassir Abbas 
Published: July 2024

AbstractEnergy serves as a crucial input in almost every sector of the economy and thus contributes significantly to the development of an economy. However, energy is obtained chiefly from fossil fuels, the world's biggest source of carbon emissions and climate change. Therefore, transforming the energy sector by promoting energy innovation is crucial and requires massive financial support from financial institutions beyond borders. In this regard, an international collaboration between the financial sectors of various economies can increase the availability of funds for investment in energy innovation. Therefore, this study intends to investigate the role of financial openness in promoting energy innovation in different regions of the world, such as Asia, America, and Europe. For regression analysis, the study employs the linear and non‐linear CS‐ARDL methods of estimations. The key outcomes of the long‐run linear analysis suggest that financial openness promotes energy innovation in all regions. In the non‐linear analysis, the positive change in financial openness increases long‐run energy innovation in all regions, while a negative change in financial openness reduces energy innovation in Global and Asia models. These findings imply that policymakers across the globe should focus on collaboration between the financial sectors to promote investment in energy innovation.
The water content, apoptosis, and proliferation of the brain in marine medaka affected by seawater acidification
Authors: XIE Jinling , LI Baolin , ZHOU Tangjian , WANG Xiaojie 
Published: July 2024

AbstractA possible explanation for ocean acidification‐induced changes in fish behavior is a systemic effect on the nervous system. Three biological barriers at the blood–brain interface effectively separate the brain from the body fluids. It is not known whether fish brain regions in contact with these barriers are affected by acidification. Here, we studied structural changes in medaka (Oryzias melastigma) brain regions contacting cerebrospinal fluid (CSF) after short‐term (7 days) CO2 exposure. The brain water content decreased significantly and the superficial structure of the pia mater was changed, but there was no obvious damage to the internal structures of the brain after seawater acidification. Seawater acidification also led to an increase in apoptosis and a decrease in the number of proliferative cells in brain areas contacting CSF. These results indicate that the structure of CSF‐contacting brain regions in medaka was affected by seawater acidification, and the brain responded to seawater acidification stress by increasing apoptosis and reducing proliferation.
Evaluation of Taiwan's IC industry production and market efficiencies under the consideration of corporate social responsibility
Authors: Lin Tai‐Yu , Chiang Hsiao‐Wen , Chiu Yung‐Ho , Chang Tzu‐Han , Liu Chung‐Tzer 
Published: July 2024

AbstractTaiwan has a complete semiconductor industry chain and is important in global semiconductor manufacturing. In addition to considering operational conditions, companies have also attached importance to corporate social responsibility (CSR) in recent years. This research thus takes 60 integrated circuit (IC) companies in Taiwan as a research sample and adopts the Meta Two‐stage dynamic RDM DDF (range directional model directional distance function) under an exogenous CSR model to explore their market stage efficiency and production stage efficiency. This study aimed to analyze whether incorporating a company's CSR performance into performance evaluations affects the production and profitability efficiency of Taiwanese IC companies. The findings are as follows: Most IC companies practice CSR and demonstrate good CSR performance. The market stage efficiency and production stage efficiency of the IC packaging and testing group are better than those of other groups.
Supporting Carers to Coregulate With Children in Care: Learnings From Action Research With Caseworkers
Authors: Ciftci Sarah , Collings Susan , Buratti Sue , Wright Amy Conley 
Published: July 2024

ABSTRACTTrauma negatively impacts on the ability of children in out‐of‐home care to experience safety in their relationships. Trauma rearousal can continue to occur even when children are living in a safe and stable setting due to environmental triggers. Carers who are emotionally regulated themselves can model and support emotional regulation (known as coregulation) when their child becomes dysregulated. To do this, carers need agencies to provide trauma‐informed and therapeutic models of care, so they in turn can offer an emotionally secure experience for their child. This article reports on participatory action research with caseworkers from two nongovernment and one government organisation who supported foster and kinship carers to coregulate with children in long‐term care. Reflective practice meetings were held over an eight‐month period to capture the perspectives and experiences of 16 caseworkers who trialled practice changes for coregulation. An inductive analysis approach was used to elicit themes. Findings revealed a three‐phase process took place for caseworkers and carers to (1) acknowledge the presence of trauma and stress, (2) become aware of their own emotional capacity and (3) apply coregulation strategies. This process was possible when organisations promoted trauma awareness and relational safety, thereby creating a ‘holding environment’ for their caseworkers and allowing coregulation to be experienced by carers and their children.
Radiographical diagnostic competences of dental students using various feedback methods and integrating an artificial intelligence application—A randomized clinical trial
Authors: Rampf Sarah , Gehrig Holger , Möltner Andreas , Fischer Martin R. , Schwendicke Falk , Huth Karin C. 
Published: July 2024

AbstractIntroductionRadiographic diagnostic competences are a primary focus of dental education. This study assessed two feedback methods to enhance learning outcomes and explored the feasibility of artificial intelligence (AI) to support education.Materials and MethodsFourth‐year dental students had access to 16 virtual radiological example cases for 8 weeks. They were randomly assigned to either elaborated feedback (eF) or knowledge of results feedback (KOR) based on expert consensus. Students´ diagnostic competences were tested on bitewing/periapical radiographs for detection of caries, apical periodontitis, accuracy for all radiological findings and image quality. We additionally assessed the accuracy of an AI system (dentalXrai Pro 3.0), where applicable. Data were analysed descriptively and using ROC analysis (accuracy, sensitivity, specificity, AUC). Groups were compared with Welch's t‐test.ResultsAmong 55 students, the eF group by large performed significantly better than the KOR group in detecting enamel caries (accuracy 0.840 ± 0.041, p = .196; sensitivity 0.638 ± 0.204, p = .037; specificity 0.859 ± 0.050, p = .410; ROC AUC 0.748 ± 0.094, p = .020), apical periodontitis (accuracy 0.813 ± 0.095, p = .011; sensitivity 0.476 ± 0.230, p = .003; specificity 0.914 ± 0.108, p = .292; ROC AUC 0.695 ± 0.123, p = .001) and in assessing the image quality of periapical images (p = .031). No significant differences were observed for the other outcomes. The AI showed almost perfect diagnostic performance (enamel caries: accuracy 0.964, sensitivity 0.857, specificity 0.074; dentin caries: accuracy 0.988, sensitivity 0.941, specificity 1.0; overall: accuracy 0.976, sensitivity 0.958, specificity 0.983).ConclusionElaborated feedback can improve student's radiographic diagnostic competences, particularly in detecting enamel caries and apical periodontitis. Using an AI may constitute an alternative to expert labelling of radiographs.
GRDATFusion: A gradient residual dense and attention transformer infrared and visible image fusion network for smart city security systems in cloud and fog computing
Authors: Zheng Jian , Jeon Seunggil , Yang Xiaomin 
Published: July 2024

AbstractThe infrared and visible fusion technology holds a pivotal position in smart city for cloud and fog computing, particularly in security system. By fusing infrared and visible image information, this technology enhances target identification, tracking and monitoring precision, bolstering overall system security. However, existing deep learning‐based methods rely heavily on convolutional operations, which excel at extracting local features but have limited receptive fields, hampering global information capture. To overcome this difficulty, we introduce GRDATFusion, a novel end‐to‐end network comprising three key modules: transformer, gradient residual dense and attention residual. The gradient residual dense module extracts local complementary features, leveraging a dense‐shaped network to retain potentially lost information. The attention residual module focuses on crucial input image details, while the transformer module captures global information and models long‐range dependencies. Experiments on public datasets show that GRDATFusion outperforms state‐of‐the‐art algorithms in qualitative and quantitative assessments. Ablation studies validate our approach's advantages, and efficiency comparisons demonstrate its computational efficiency. Therefore, our method makes the security systems in smart city with shorter delay and satisfies the real‐time requirement.
Role of short chain fatty acids on astrocytes and microglia in Alzheimer's disease brain
Authors: Senarath Rathnayaka Mudiyanselage Uththara Sachinthanie , Burton Matthew , Fernando Welisarage Mary Kendrea Thiasangee , Jayasena Vijay , Brennan Charles , Fernando Wanakulasuriya Mary Ann Dipika Binosha 
Published: July 2024

SummaryShort‐chain fatty acids (SCFAs) hold exert many nutritional benefits on both physiological and pathological processes, including host metabolism, immune modulation, and appetite control. Originating from the microbial fermentation of resistant starches and dietary fibre in the colon, SCFAs have the capability to traverse the blood–brain barrier (BBB). It is evident that SCFAs play a critical role in the development of neurodegenerative disorders such as Alzheimer's disease (AD). AD is the most common form of dementia and is characterised by the deposition of amyloid β peptides (Aβ) and tau hyperphosphorylation. SCFAs have demonstrated the potential to interfere with the protein–protein interactions required for Aβ peptides to form neurotoxic oligomers. Moreover, research indicates that the SCFAs ability to maintain endothelial integrity and interact with neuroglial cells through the BBB may assist in downregulating excessive neuroinflammatory responses to amyloid peptides. This review summarises the research on SCFAs' potential role in AD and understand their mechanism of actions on astrocytes and microglia.
Profiling the effects of starter cultures on biochemical compounds in fermented fish sauces and their relationships with sensory perceptions
Authors: Dinc Selin Ozge , Colakoglu Fatma , Kunili Ibrahim Ender , Ormanci Hasan Basri 
Published: July 2024

SummaryIn this study, the effect of Lactiplantibacillus (L.) plantarum XL23 (Lp) and Saccharomyces (S.) cerevisiae RC212 (Sc) on flavour formation in fermented fish sauce production was investigated. The levels of, and relationship between, non‐volatile and volatile compounds responsible for taste and aroma were determined in fish sauce samples. Regarding non‐volatiles, the results showed that free amino acids and organic acids were significantly higher in Lp and Lp + Sc compared to traditional fish sauce. In fatty acids, there was an irregular distribution between the groups. In terms of volatile compounds, L. plantarum supported the presence of acidic compounds (1413.31 ng mL−1), while S. cerevisiae supported the presence of alcoholic compounds at high levels (3891.56 ng mL−1). Significant correlations between components proved the accuracy of analytical and sensory analyses and demonstrated the reliability of multi‐replicate statistical interactions. The results indicated that inoculation with starter cultures changed the taste and aroma in favour of the strains.
Valorisation of banana peel and mango peel as functional ingredients in baked products: a review
Authors: Ismail Sahal , Dubey Praveen Kumar , Mishra Atul Anand , Ashka Fathima 
Published: July 2024

SummaryThe baking industry is greatly interested in incorporating banana peel and mango peel into a broader spectrum of baked goods. This review delves into the innovative utilisation of banana and mango peels in various baked products, specifically focusing on biscuits, cookies, cakes, and cake doughnuts. Incorporating banana peel in baked goods has gained attention for its potential to enhance flavour, texture, and nutritional value. Similarly, mango peel has been explored for its unique properties and potential contributions to baked items. This review emphasises the health benefits of banana and mango peels, including antioxidants, fibre, and other nutritional advantages that help prevent diseases and promote overall well‐being. Limited scientific studies may explore using banana peel and mango peel specifically as functional ingredients in baked products. This review highlights the utilisation of banana peel and mango peel in baked products, focusing on their influence on product quality, sensory characteristics, and potential health benefits. It presents a sustainable and innovative approach to creating healthier, more flavourful baked products.