bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGEMBANGAN E-MODUL TERINTEGRASI HOTS MATERI GARIS DAN SUDUT UNTUK MENINGKATKAN LITERASI NUMERASI SISWA KELAS VII SMP
Authors: Winanda Mutia Balkis , Reflina  
Published: August 2024

Penelitian pengembangan ini bertujuan untuk menghasilkan e-modul terintegrasi HOTS yang valid, praktis dan efektif untuk meningkatkan literasi numerasi siswa kelas VII SMP Pahlawan Nasional. Produk yang dikembangkan berupa e-modul. Penelitian ini menggunakan metode Research and Development dengan model ADDIE yaitu Analysis, Design, Development, Implementation, dan Evaluation. Hasil penelitian pada uji validitas e-modul masuk dalam kategori sangat valid dengan rata-rata 85,69%, hasil kepraktisan e-modul melalui angket respon guru dengan persentase 95% kategori sangat praktis, dan angket respon siswa dengan persentase 88,17% dengan kategori sangat praktis, hasil tes menunjukkan adanya peningkatan kemampuan literasi numerasi siswa, dengan hasil 0,9 adalah kategori efektif. Sehingga e-modul terintegrasi higher order thinking skills pada materi garis dan sudut untuk meningkatkan literasi numerasi siswa valid, praktis dan efektif untuk digunakan.
Analysis of Corporate University Programs and Their Relevance to E-Government Implementation in Indonesia
Authors: Wijaya I Made Aldy , Alle Elkis , Sudarmono Sudarmono 
Published: August 2024

Today's digitalization era demands system transformation in all sectors of life, including the government and bureaucratic sectors. State officials’ competence needs to be improved in order to adjust to the digital government environment (e-government). Education and training are essential for coping with the dynamic environment and enhancing the competency management of Civil Servants (ASN), as mandated by Law Number 5 of 2014 concerning ASN. This necessity has led to the emergence of the Corporate University concept in education and training units. This research aims to assess the relevance of the Corporate University program in developing ASN competencies to meet the challenges of e-government in Indonesia. The research employs descriptive methods and qualitative approaches, with data sourced from literature reviews. Data collection is conducted through documentation methods, and problem analysis utilizes Miles & Huberman's data analysis techniques, which include data reduction, data presentation, and conclusion drawing. The findings indicate that the Corporate University program is pertinent to the implementation of e-government, particularly in capacity building for civil servants through integrative learning and e-learning-based training in government agencies. Corporate University can be an effective solution to the need for digitally literate civil servants capable of operating an integrated digital service system, especially within local government, which is the closest government unit to the community.
THE VALIDITY OF STUDENT WORKSHEET BASED PROJECT IN TRAINING STUDENTS’ CRITICAL THINKING FOR 8th MTs ON EXCRETION SYSTEM MATERIALS
Authors: Zuhrotun Nisa'  , Nurhayati  
Published: August 2024

The purpose of this study is to develop project based learning worksheets on excretion system material for class VIII MTs based on validity test. In project based learning, excretory system material is material connected to life. This is a form of development research with reference to the ADDIE development model, which has 5 stages: (1) Analyze, (2) Design, (3) Development, (4) Implementation, and (5) Evaluation. However, the Implementation did not take place in this research. The acquired data was descriptively examined quantitatively and qualitatively. The outcomes of the LKPD development are consistent with the revisions of the supervising lecturers and validators consisting of media expert lecturers, subject matter expert lecturers, and science teachers. Based on the outcomes of the development assessment of project based learning worksheet obtained an average validation result from the validator of 3,68 with a very valid category. So, it can be conclude that the development of project based learning worksheets on excretion system material for class VIII MTs. which developed with the ADDIE development model is very valid for use in learning.
Comprehensive assessment of microalgal-based treatment processes for dairy wastewater
Authors: Singh Pooja , Mohanty Satya Sundar , Mohanty Kaustubha 
Published: August 2024

The dairy industry is becoming one of the biggest sectors within the global food industry, and these industries use almost 34% of the water. The amount of water used is governed by the production process and the technologies employed in the plants. Consequently, the dairy industries generate almost 0.2–10 L of wastewater per liter of processed milk, which must be treated before being discharged into water bodies. The cultivation of microalgae in a mixotrophic regime using dairy wastewater enhances biomass growth, productivity, and the accumulation of value-added product. The generated biomass can be converted into biofuels, thus limiting the dependence on petroleum-based crude oil. To fulfill the algal biorefinery model, it is important to utilize every waste stream in a cascade loop. Additionally, the harvested water generated from algal biomass production can be recycled for further microalgal growth. Economic and sustainable wastewater management, along with proper reclamation of nutrients from dairy wastewater, is a promising approach to mitigate the problem of water scarcity. A bibliometric study revealing limited work on dairy wastewater treatment using microalgae for biofuel production. And, limited work is reported on the pretreatment of dairy wastewater via physicochemical methods before microalgal-based treatment. There are still significant gaps remains in large-scale cultivation processes. It is also crucial to discover robust strains that are highly compatible with the specific concentration of contaminants, as this will lead to increased yields and productivity for the targeted bio-product. Finally, research on reutilization of culture media in photobioreactor is necessary to augument the productivity of the entire process. Therefore, the incorporation of the microalgal biorefinery with the wastewater treatment concept has great potential for promoting ecological sustainability.
Cascading renal injury after brain death: Unveiling glycocalyx alteration and the potential protective role of tacrolimus
Authors: Idouz Kaoutar , Belhaj Asmae , Rondelet Benoit , Dewachter Laurence , Flamion Bruno , Kirschvink Nathalie , Dogné Sophie 
Published: August 2024

Brain death (BD) is a complex medical state that triggers systemic disturbances and a cascade of pathophysiological processes. This condition significantly impairs both kidney function and structural integrity, thereby presenting considerable challenges to graft viability and the long-term success of transplantation endeavors. Tacrolimus (FK506), an immunosuppressive drug, was used in this study to assess its impact as a pretreatment on brain death-induced renal injury. This study aimed to investigate changes associated with brain death-induced renal injury in a 4-month-old female porcine model. The experimental groups included brain death placebo-pretreated (BD; n = 9), brain death tacrolimus-pretreated using the clinical dose of 0.25 mg/kg the day before surgery, followed by 0.05 mg/kg/day 1 hour before the procedure (BD + FK506; n = 8), and control (ctrl, n = 7) piglets, which did not undergo brain death induction. Furthermore, we aimed to assess the effect of FK506 on these renal alterations through graft preconditioning. We hypothesized that immunosuppressive properties of FK506 reduce tissue inflammation and preserve the glycocalyx. Our findings revealed a series of interconnected events triggered by BD, leading to a deterioration of renal function and increased proteinuria, increased apoptosis in the vessels, glomeruli and tubules, significant leukocyte infiltration into renal tissue, and degradation of the glycocalyx in comparison with ctrl group. Importantly, treatment with FK506 demonstrated significant efficacy in attenuating these adverse effects. FK506 helped reduce apoptosis, maintain glycocalyx integrity, regulate neutrophil infiltration, and mitigate renal injury following BD. This study offers new insights into the pathophysiology of BD-induced renal injury, emphasizing the potential of FK506 pretreatment as a promising therapeutic intervention for organ preservation, through maintaining endothelial function with the additional benefit of limiting the risk of rejection.
Structural insights of an LCP protein–LytR–from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods
Authors: Paquete-Ferreira João , Freire Filipe , Fernandes Henrique S. , Muthukumaran Jayaraman , Ramos João , Bryton Joana , Panjkovich Alejandro , Svergun Dmitri , Santos Marino F. A. , Correia Márcia A. S. , Fernandes Alexandra R. , Romão Maria João , Sousa Sérgio F. , Santos-Silva Teresa 
Published: August 2024

The rise of antibiotic-resistant bacterial strains has become a critical health concern. According to the World Health Organization, the market introduction of new antibiotics is alarmingly sparse, underscoring the need for novel therapeutic targets. The LytR-CpsA-Psr (LCP) family of proteins, which facilitate the insertion of cell wall glycopolymers (CWGPs) like teichoic acids into peptidoglycan, has emerged as a promising target for antibiotic development. LCP proteins are crucial in bacterial adhesion and biofilm formation, making them attractive for disrupting these processes. This study investigated the structural and functional characteristics of the LCP domain of LytR from Streptococcus dysgalactiae subsp. dysgalactiae. The protein structure was solved by X-ray Crystallography at 2.80 Å resolution. Small-angle X-ray scattering (SAXS) data were collected to examine potential conformational differences between the free and ligand-bound forms of the LytR LCP domain. Additionally, docking and molecular dynamics (MD) simulations were used to predict the interactions and conversion of ATP to ADP and AMP. Experimental validation of these predictions was performed using malachite green activity assays. The determined structure of the LCP domain revealed a fold highly similar to those of homologous proteins while SAXS data indicated potential conformational differences between the ligand-free and ligand-bound forms, suggesting a more compact conformation during catalysis, upon ligand binding. Docking and MD simulations predicted that the LytR LCP domain could interact with ADP and ATP and catalyze their conversion to AMP. These predictions were experimentally validated by malachite green activity assays, confirming the protein’s functional versatility. The study provides significant insights into the structural features and functional capabilities of the LCP domain of LytR from S. dysgalactiae subsp. dysgalactiae. These findings pave the way for designing targeted therapies against antibiotic-resistant bacteria and offer strategies to disrupt bacterial biofilm formation.
Exploring the causality of appendectomy and ischaemic heart disease: a Mendelian randomization study and meta-analysis
Authors: Wang Shuai , Zhang Tao , Sun Yuanlin , Yao Yiwei , Yang Dongliang , Cao Xueyuan 
Published: August 2024

BackgroundThe risk of ischaemic heart disease (IHD) is increased in appendectomy patients, but it is not clear whether there is a causal relationship. We aimed to systematically estimate the causal relationship between appendectomy and IHD and its subtypes, acute myocardial infarction (AMI) and angina pectoris (AP), using Mendelian randomization (MR) study methods and meta-analysis.MethodsAs the discovery cohort analysis, we extracted independent genetic variants strongly associated with appendectomy from the FinnGen study (28,601 cases) as instrumental variables (IVs). Genome-wide association study (GWAS) from UK Biobank were selected for outcome data. A first two-sample MR analysis was then conducted. As the replication cohort, IVs associated with appendectomy were extracted in the UK Biobank (50,105 cases). GWAS from the FinnGen study were selected for outcome data. A second MR analysis was then performed. Finally, meta-analyses were applied to assess the combined causal effects of the MR results.ResultsIn the discovery cohort, there was a significant positive causal relationship between appendectomy and IHD and its subtypes AMI and AP. The replication cohort only found a positive causal relationship between appendectomy and AMI. Meta-analysis showed a positive causal relationship between appendectomy and IHD (OR: 1.128, 95% CI: 1.067–1.193, P = 2.459e-05), AMI (OR: 1.195, 95% CI: 1.095–1.305, P = 6.898e-05), and AP (OR: 1.087, 95% CI: 1.016–1.164, P = 1.598e-02).ConclusionsThis comprehensive MR analysis suggests that genetically predicted appendectomy may be a risk factor for the development of IHD and its subtypes AMI and AP. We need to continue to pay attention to these links.
Development and validation of a predictive nomogram for the risk of MAFLD in postmenopausal women
Authors: Yang Ming , Chen Xingyu , Shen Qiaohui , Xiong Zhuang , Liu Tiejun , Leng Yan , Jiao Yue 
Published: August 2024

Background and aimMetabolic-associated fatty liver disease (MAFLD) has gradually become one of the main health concerns regarding liver diseases. Postmenopausal women represent a high-risk group for MAFLD; therefore, it is of great importance to identify and intervene with patients at risk at an early stage. This study established a predictive nomogram model of MAFLD in postmenopausal women and to enhance the clinical utility of the new model, the researchers limited variables to simple clinical and laboratory indicators that are readily obtainable.MethodsData of 942 postmenopausal women from January 2023 to October 2023 were retrospectively collected and divided into two groups according to the collection time: the training group (676 cases) and the validation group (226 cases). Significant indicators independently related to MAFLD were identified through univariate logistic regression and stepwise regression, and the MAFLD prediction nomogram was established. The C-index and calibration curve were used to quantify the nomogram performance, and the model was evaluated by measuring the area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA).ResultsOf 37 variables, 11 predictors were identified, including occupation (worker), body mass index, waist-to-hip ratio, number of abortions, anxiety, hypertension, hyperlipidemia, diabetes, hyperuricemia, and diet (meat and processed meat). The C-index of the training group predicting the related risk factors was 0.827 (95% confidence interval [CI] 0.794–0.860). The C-index of the validation group was 0.787 (95% CI 0.728–0.846). Calibration curves 1 and 2 (BS1000 times) were close to the diagonal, showing a good agreement between the predicted probability and the actual incidence in the two groups. The AUC of the training group was 0.827, the sensitivity was 0.784, and the specificity was 0.735. The AUC of the validation group was 0.787, the sensitivity was 0.674, and the specificity was 0.772. The DCA curve showed that the nomogram had a good net benefit in predicting MAFLD in postmenopausal women.ConclusionsA predictive nomogram for MAFLD in postmenopausal women was established and verified, which can assist clinicians in evaluating the risk of MAFLD at an early stage.
Enhanced recovery after surgery in elective cesarean section patients with gestational diabetes mellitus does not lead to glucose-related maternal and neonatal complications
Authors: Zhou Jin , Zhang Peizhen , Tan Zhangmin , Li Chuo , Yao Lin , He Tiantian , Hou Hongyin , Yin Yuzhu 
Published: August 2024

ObjectiveFor elective cesarean section patients with gestational diabetes mellitus (GDM), there is a lack of evidence-based research on the use of enhanced recovery after surgery (ERAS). This study aims to compare the ERAS after-surgery protocol and traditional perioperative management.Research design and methodsIn this retrospective cohort study, singleton pregnancies with good glucose control GDM, delivered by elective cesarean sections under intravertebral anesthesia at least 37 weeks from January 1 to December 31, 2022, were collected at the Third Affiliated Hospital of Sun Yat-sen University. We divided all enrolled pregnant women and newborns into an ERAS group and a control group (the traditional perioperative management group) based on their adherence to the ERAS protocol. The primary outcome was the preoperative blood glucose level, with an increase of more than 1 mmol/L indicating clinical significance when compared to the control group. The secondary outcome was centered around an adverse composite outcome that affected both mothers and newborns.ResultsWe collected a total of 161 cases, with 82 in the ERAS group and 79 in the control group. Although the mean preoperative blood glucose level in the ERAS group was significantly higher than in the control group (5.01 ± 1.06 mmol/L vs. 4.45 ± 0.90 mmol/L, p&lt;0.001), the primary outcome revealed that the mean glycemic difference between the groups was 0.47 mmol/L (95% CI 0.15-0.80 mmol/L), which was below the clinically significant difference of 1 mmol/L. For the secondary outcomes, the ERAS group had an 86% lower risk of a composite adverse outcome compared to the control group. This included a 73% lower risk of perioperative maternal hypoglycemia and a 92% lower rate of neonatal hypoglycemia, all adjusted by age, hypertensive disorder of pregnancy, BMI, gestational weeks, primigravidae, primary pregnancy, GDM, surgery duration, and fasting glucose.ConclusionImplementing a low-dose carbohydrate ERAS in pregnant women with GDM prior to elective cesarean section, compared to traditional perioperative management, does not lead to clinically significant maternal glucose increases and thus glucose-related maternal or neonatal perioperative complications.
Microwave ablation for lymph node metastasis in thyroid cancer: the impact of lymph node diameter
Authors: Xiao Xiaoyi , Chen Xi , Li Jingwei , Li Pei , Zhu Yun 
Published: August 2024

ObjectivesTo explore the impact of lymph node diameter on the efficacy and safety of ultrasound-guided microwave ablation (MWA) in the treatment of cervical metastatic lymph nodes (CMLNs) from thyroid cancer.MethodsA total of 32 patients with 58 CMLNs from thyroid cancer underwent ultrasound-guided MWA and were included in the retrospective study. Patients were divided into three groups based on the mean largest diameter of the CMLNs: Group A (diameter ≤10mm), Group B (10mm &lt; diameter ≤20mm), and Group C (diameter &gt;20mm). The research involved comparing changes in cervical metastatic lymph nodes and serum thyroglobulin (sTg) levels, as well as the incidence of complications, before and after microwave ablation across three groups of patients.ResultsThe technical success rate of this study was 100% (32/32), and they showed no major complications. Compared with measurements taken before MWA, the mean largest diameter and volume of CMLNs, as well as the sTg level, showed significant reductions (p &lt;0.05) at the last follow-up in all three patient groups. Group A and B exhibited higher lymph node volume reduction rates and complete disappearance rates compared to Group C. However, the recurrence rate in the three groups were in the following order: Group C &gt; Group B &gt; Group A. The occurrence rate of mild complications was Group A &gt; Group C &gt; Group B.ConclusionMWA is a safe and effective method for treating CMLNs, with advantages for localized nodes but limitations for larger ones. Careful consideration and personalized plans are advised, based on comprehensive evidence assessment.
Paraben residues in wastewater and surface water: a case study of KwaZulu Natal and Gauteng provinces (South Africa) during the COVID-19 pandemic
Authors: Mpayipheli Neliswa , Mpupa Anele , Madala Ntakadzeni Edwin , Nomngongo Philiswa Nosizo 
Published: August 2024

Introduction: The presence of pharmaceuticals and personal care products in environmental matrices is considered one of the major scientific concerns. Most of these substances are disposed of unchanged through wastewater treatment plants and sewage systems. Consequently, they are continuously introduced into the water systems and progressively contaminate surface, ground and drinking water. During the COVID-19 pandemic, a large number of emerging contaminants including parabens were released to the environment through various routes. In this study, the occurrence of parabens (methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB) was investigated in wastewater samples from various wastewater treatment plants (WWTPs) and the receiving surface waters in KwaZulu Natal and Gauteng Provinces (South Africa).Methods: The samples were collected between October 2020 and December 2021, covering the 2nd, 3rd and 4th waves of the COVID-19 pandemic. A solid phase extraction protocol with high-performance liquid chromatography was used to extract and enrich parabens before analysis.Results and Discussion: Methylparaben (2.02–84.7 μg/L), EtPB (&lt;0.24–24.8 μg/L), PrPB (&lt;0.26–55.1 μg/L), and BuPB (&lt;0.27–17.3 μg/L) were quantified in wastewater influent collected WWTPs of KwaZulu Natal Province. While &lt;0.19–5.43 μg/L, &lt;0.16–5.63 μg/L, &lt;0.17–6.89 μg/L, and &lt;0.19–5.32 μg/L for MePB, EtPB, PrPB, and BuPB, respectively, were quantified in effluent wastewater from the same province. The concentrations of MePB, EtPB, PrPB, and BuPB in influent wastewater from Gauteng Province were 2.58–123 μg/L, &lt;0.24–33.6 μg/L, 3.77–73.4 μg/L and &lt;0.27–85.8 μg/L, respectively. In effluent wastewater, concentrations ranging from 0.24–17.76 μg/L (MePB), &lt;0.16–4.88 μg/L (EtPB), 0.69-12.5 μg/L (PrPB), and &lt;0.19–4.726 μg/L (BuPB) were quantified. During the 4th wave, the concentrations of parabens in surface water were lower compared to the second and third waves of the pandemic. In general, the paraben residues in the surface of KwaZulu Natal Province (&lt;0.08–16.4 μg/L) were higher than those in Gauteng Province (0.08-3.14 µg/L). Methylparaben and propylparaben were dominant in all investigated samples (wastewater and surface water), followed by ethylparaben. The ecotoxicological risk assessment was carried out for aquatic biota, which was estimated in terms of risk quotients (RQs). RQs for the target compounds in river water indicated that MePB and EtPB pose low risk, whereas PrPB and BuPB pose low to medium risk to aquatic organisms.