bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photoprotective Potential of Passiflora Cincinnata Mast. (Passifloraceae) Hydro‐Alcoholic Extracts
Authors: de Oliveira Júnior Raimundo Gonçalves , Reis Silvio Alan Gonçalves Bonfim , de Oliveira Ana Paula , Ferraz Christiane Adrielly Alves , Rolim Larissa Araújo , Lopes Norberto Peporine , Rocha Joao Miguel , El Aouad Noureddine , Kritsanida Marina , Almeida Jackson Roberto Guedes da Silva 
Published: August 2024

AbstractPassiflora cincinnata is a Passifloraceae typical of the Caatinga, a biome unique to Brazil. It has various pharmacological properties associated with its high flavonoid content. Vitexin, isovitexin, orientin, isoorientin and derivatives are the main chemical and pharmacological markers for this plant. Although flavonoids enriched‐extracts have been widely applied in phytocosmetics, especially in sunscreen formulations, the use of P. cincinnata as a photoprotective ingredient remains unexplored. Different hydro‐alcoholic extracts were prepared and their antioxidant and photoprotective activities were evaluated by in vitro assays. The most promising extract (Pc‐1) was analyzed by HPLC‐DAD‐ESI‐MS/MS. Nine flavonoids were identified as major compounds: isovitexin‐7‐O‐glucoside, isoorientin‐2”O‐hexoside, orientin, isoorientin, isovitexin‐2”‐O‐glucoside, isovitexin‐6”‐O‐glucoside, isoscoparin and isoquercitrin. Finally, Pc‐1 (5 and 10 %, v/v) was incorporated into gel formulations, alone or combined to commercial chemical filters (benzophenone‐3 and octyl methoxycinnamate). Formulations containing Pc‐1 showed high SPFspectrophotometric values. When combined to commercial filters, Pc‐1 (5 %) potentiated their photoprotective efficacy (p<0.05). A physicochemical characterization indicated no incompatibility or signs of instability after extract incorporation. Altogether, these findings encourage the use of Pc‐1 as a photoprotective ingredient or co‐adjuvant in sunscreens formulations.
Specialized Metabolites from the Husks of Rice Oryza Sativa L. and their Biological Activities
Authors: Zhang Xue‐ying , Ge Cheng , Zhang Ai‐ju , Wu Si‐xuan , Yang Min , Huang Lei , Li Jian Lin 
Published: August 2024

AbstractRice (Oryza sativa L.) husk harbors a substantial proportion of biological metabolites, as one of the most plentiful agriculture by‐products in rice milling process, rice husk remains poorly utilized. As a continuing search for potential bioactive molecules from the husk of rice, a totally of twelve components (1–12), including six sterol ferulates (1–6), one flavonoid (7), one dipeptide (8), and four phenylpropanoid derivatives (9–12) were obtained. All the chemical structures were elucidated based on comprehensive spectroscopic data. Wherein, compounds 1 and 2 were yield as previous undescribed metabolites, and the comprehensive NMR data for compounds 3 and 4 were first presented in its entirety. Motivated by the similarity of the structural motifs of components 1–6 to that of reported sterol ferulates, the antioxidant and anti‐inflammatory effects for compounds 1–6 were evaluated in vitro. Among them, compounds 5/6 had a significant antioxidant activity compare to that of vitamin E in both DPPH and reducing power assay up to the concentration 40 μg/ml; while compounds 1 and 2 exhibited weak suppressive effect on the production of nitric oxide, with the IC50 values of 53.27±1.37 μM.
Maxillary impacted canine replacing a central incisor with root resorption: Multidisciplinary treatment in a preadolescent patient
Authors: Wen Li‐Ming , Song Yang‐Yang , Liu Ji‐Nan , Zhu Ye , Huang Xiao‐Feng 
Published: August 2024

Key Clinical MessageMaxillary canines are often impacted, which can result in tooth disorders and adversely affect occlusal and facial development. The case report describes complete bilateral impaction of maxillary canines and significant root resorption of a central incisor. The multidisciplinary approach is the optimal strategy for addressing impacted maxillary canines.
Role of primary care in opioid prescribing for older head and neck cancer survivors
Authors: Salz Talya , Meza Akriti Mishra , Bradshaw Patrick T. , Jinna Sankeerth , Moryl Natalie , Kriplani Anuja , R. Tringale Kathryn , Flory James , Korenstein Deborah , Lipitz‐Snyderman Allison 
Published: July 2024

AbstractBackgroundOlder head and neck cancer (HNC) survivors have concerning rates of potentially unsafe opioid prescribing. Identifying the specialties of opioid prescribers for HNC survivors is critical for targeting the settings for opioid safety interventions. This study hypothesized that oncology and surgery providers are primarily responsible for opioid prescriptions in the year after treatment but that primary care providers (PCPs) are increasingly involved in prescribing over time.MethodsUsing linked Surveillance, Epidemiology, and End Results–Medicare data, a retrospective analysis was conducted of adults aged >65 years diagnosed between 2014 and 2017 with stage I–III HNC and who had ≥6 months of treatment‐free follow‐up through 2019. Starting at treatment completion, opioid fills were assigned to a prescriber specialty: oncology, surgery, primary care, pain management, or other. Prescriber patterns were summarized for each year of follow‐up. Multinomial logistic regression models captured the likelihood of opioids being prescribed by each specialty.ResultsAmong 5135 HNC survivors, 2547 (50%) had ≥1 opioid fill (median, 2.1‐year follow‐up). PCPs prescribed 47% of all fills (42%–55% each year). PCPs prescribed opioids to 45% of survivors with ≥1 opioid fill, which was a greater share than other specialties. PCPs prescribed longer supplies of opioids (median, 20 days/fill; median, 30 days/year) than oncologists or surgeons. The likelihood of an opioid being prescribed by an oncology provider was four times lower than that of it being prescribed by a PCP.ConclusionsPCP involvement in opioid prescribing remains high throughout HNC survivorship. Interventions to improve the safety of opioid prescribing should target primary care, as is typical for opioid reduction efforts in the noncancer population.
Bacterial surface derived extracellular vesicles: A ground‐breaking approach in cancer therapy
Authors: Das Asmit , Sonar Swarup , Kalele Ketki , Subramaniyan Vetriselvan 
Published: July 2024

AbstractConventional cancer therapies are often limited by inherent and acquired drug resistance, non‐specific toxicity, and suboptimal drug delivery. Bacterial extracellular vesicles (BEVs), including outer membrane vesicles and membrane vesicles, present a novel therapeutic strategy for cancer treatment. BEVs were found in both Gram‐negative and Gram‐positive bacteria. This article provides a classified investigation of the therapeutic potential of BEVs in cancer treatment. We discuss the molecular mechanisms underlying their observed anti‐tumor effects, including the induction of apoptosis in cancer cells, suppression of angiogenesis within the tumor microenvironment, and stimulation of both innate and adaptive anti‐tumor immune responses. Moreover, BEVs being biocompatible opens avenue for targeted drug delivery systems, potentially improving the therapeutic index of conventional chemotherapeutics. Challenges to clinical translation, such as BEV heterogeneity and potential immunogenicity, are addressed. We also explore future directions for research and development, comprehensively highlighting how BEVs transform the landscape of precision oncology and cancer treatment, eventually improving patient outcomes.
High‐fidelity CRISPR/Cas12a dual‐crRNA screening reveals novel synergistic interactions in hepatocellular carcinoma
Authors: Chen Qian , Pang Minhui , Chen Peng , Zhou Zihao , Lei Jun , He Boxiao , Sun Zaiqiao , Paek Chonil , Jing Baowei , Wu Yankang , Liu Shiqi , Chen Yongshun , Yin Lei 
Published: July 2024

Abstract CRISPR/Cas12a‐based combinational screening has shown remarkable potential for identifying genetic interactions. Here, we describe an innovative method for combinational genetic screening with rapid construction of a dual‐CRISPR RNA (crRNA) library using gene splicing through overlap extension PCR (SOE PCR) and the adoption of CeCas12a, which we previously identified with strict PAM recognition and low off‐targeting to guarantee fidelity and efficiency. The custom‐pooled SOE crRNA array (SOCA) library for double‐knockout screening could be conveniently constructed in the laboratory for widespread use, and the CeCas12a‐mediated high‐fidelity screen displayed good performance even under a negative selection screen. By designing a SOCA dual‐crRNA library that covered most of the kinase and metabolism‐associated gene targets of FDA‐approved drugs implicated in hepatocellular carcinoma (HCC) tumourigenesis, novel cross‐talk between the two gene sets was negatively selected to inhibit HCC cell growth in vitro and in vivo and was validated using virtual double‐knockdown screening based on TCGA databases. Thus, this rapid, efficient and high‐fidelity double‐knockout screening system is promising for systemically identifying potential genetic interactions between multiple gene sets or combinations of FDA‐ approved drugs for clinical translational medicine in the future.
A comprehensive overview of the molecular features and therapeutic targets in BRAFV600E‐mutant colorectal cancer
Authors: Gu Ruiqi , Fang Hongsheng , Wang Renjie , Dai Weixing , Cai Guoxiang 
Published: July 2024

AbstractAs one of the most prevalent digestive system tumours, colorectal cancer (CRC) poses a significant threat to global human health. With the emergence of immunotherapy and target therapy, the prognosis for the majority of CRC patients has notably improved. However, the subset of patients with BRAF exon 15 p.V600E mutation (BRAFV600E) has not experienced remarkable benefits from these therapeutic advancements. Hence, researchers have undertaken foundational investigations into the molecular pathology of this specific subtype and clinical effectiveness of diverse therapeutic drug combinations. This review comprehensively summarised the distinctive molecular features and recent clinical research advancements in BRAF‐mutant CRC. To explore potential therapeutic targets, this article conducted a systematic review of ongoing clinical trials involving patients with BRAFV600E‐mutant CRC.
Endotypes of severe neutrophilic and eosinophilic asthma from multi‐omics integration of U‐BIOPRED sputum samples
Authors: Kermani Nazanin Zounemat , Li Chuan‐Xing , Versi Ali , Badi Yusef , Sun Kai , Abdel‐Aziz Mahmoud I , Bonatti Martina , Maitland‐van der Zee Anke‐Hilse , Djukanovic Ratko , Wheelock Åsa , Dahlen Sven‐Erik , Howarth Peter , Guo Yike , Chung Kian Fan , Adcock Ian M. ,   U‐BIOPRED Project Team
Published: July 2024

AbstractBackgroundClustering approaches using single omics platforms are increasingly used to characterise molecular phenotypes of eosinophilic and neutrophilic asthma. Effective integration of multi‐omics platforms should lead towards greater refinement of asthma endotypes across molecular dimensions and indicate key targets for intervention or biomarker development.ObjectivesTo determine whether multi‐omics integration of sputum leads to improved granularity of the molecular classification of severe asthma.MethodsWe analyzed six ‐omics data blocks–microarray transcriptomics, gene set variation analysis of microarray transcriptomics, SomaSCAN proteomics assay, shotgun proteomics, 16S microbiome sequencing, and shotgun metagenomic sequencing–from induced sputum samples of 57 severe asthma patients, 15 mild‐moderate asthma patients, and 13 healthy volunteers in the U‐BIOPRED European cohort. We used Monti consensus clustering algorithm for aggregation of clustering results and Similarity Network Fusion to integrate the 6 multi‐omics datasets of the 72 asthmatics.ResultsFive stable omics‐associated clusters were identified (OACs). OAC1 had the best lung function with the least number of severe asthmatics with sputum paucigranulocytic inflammation. OAC5 also had fewer severe asthma patients but the highest incidence of atopy and allergic rhinitis, with paucigranulocytic inflammation. OAC3 comprised only severe asthmatics with the highest sputum eosinophilia. OAC2 had the highest sputum neutrophilia followed by OAC4 with both clusters consisting of mostly severe asthma but with more ex/current smokers in OAC4. Compared to OAC4, there was higher incidence of nasal polyps, allergic rhinitis, and eczema in OAC2. OAC2 had microbial dysbiosis with abundant Moraxella catarrhalis and Haemophilus influenzae. OAC4 was associated with pathways linked to IL‐22 cytokine activation, with the prediction of therapeutic response to anti‐IL22 antibody therapy.ConclusionMulti‐omics analysis of sputum in asthma has defined with greater granularity the asthma endotypes linked to neutrophilic and eosinophilic inflammation. Modelling diverse types of high‐dimensional interactions will contribute to a more comprehensive understanding of complex endotypes.Key Points
Unsupervised clustering on sputum multi‐omics of asthma subjects identified 3 out of 5 clusters with predominantly severe asthma.
One severe asthma cluster was linked to type 2 inflammation and sputum eosinophilia while the other 2 clusters to sputum neutrophilia.
One severe neutrophilic asthma cluster was linked to Moraxella catarrhalis and to a lesser extent Haemophilus influenzae while the second cluster to activation of IL‐22.
A novel approach for missing data recovery and fault nodes detection in wireless sensor networks
Authors: Thiyagarajan R. , Nagabhooshanam N. , Prasad K.D.V. , Poojitha P. 
Published: August 2024

SummaryEnsuring data integrity in wireless sensor networks (WSNs) is crucial for accurate monitoring, yet missing data due to sensor faults present a significant challenge. This research introduces an innovative approach that integrates advanced data recovery techniques with leading‐edge methods to address this issue. The system begins by identifying and isolating fault nodes using a specialized algorithm that analyzes network behavior. By applying fuzzy density‐based spatial clustering of applications with noise (FDBSCAN), potential fault nodes are precisely located based on deviations from expected patterns. Subsequently, an intelligent missing data recovery mechanism powered by bidirectional long short‐term memory (Bi‐LSTM) networks takes action. The Bi‐LSTM model is trained on existing sensor data to capture intricate patterns and dependencies, enabling accurate prediction and reconstruction of missing values caused by identified faults. The synergy between Bi‐LSTM for missing data recovery and FDBSCAN for fault node detection comprehensively addresses the missing data problem in WSNs. In missing data recovery, it demonstrates low mean absolute deviation (MAD) ranging from 0.021 to 0.13 and mean squared deviation (MSD) ranging from 0.0025 to 0.05 across various missing data ratios. Data reliability remains consistently high at 96% to 98%, even with up to 80% missing data. For fault node detection, the approach achieves precision of 95.7%, recall of 96.3%, F1‐score of 96.1%, and accuracy of 97.4%, outperforming existing techniques. The computational cost during training is noted at 5.79 h, presenting a limitation compared to other methods. This research highlights the importance of integrating fault node detection into missing data recovery mechanisms, presenting an innovative solution for the advancement of WSNs.
Med1 controls thymic T‐cell migration into lymph node through enhancer‐based Foxo1‐Klf2 transcription program
Authors: Yuan Ning , Su Yanhong , Gao Yang , Yang Biao , Zhang Tianzhe , Wang Qianhao , Zhang Dan , Shi Lin , Jiao Anjun , Lei Lei , Sun Lina , Zhang Baojun 
Published: July 2024

AbstractThe migration is the key step for thymic T cells to enter circulation and then lymph nodes (LNs), essential for future immune surveillance. Although promoter‐based transcriptional regulation through Foxo1, Klf2, Ccr7, and Sell regulates T‐cell migration, it remains largely unexplored whether and how enhancers are involved in this process. Here we found that the conditional deletion of Med1, a component of the mediator complex and a mediator between enhancers and RNA polymerase II, caused a reduction of both CD4+ and CD8+ T cells in LNs, as well as a decrease of CD8+ T cells in the spleen. Importantly, Med1 deletion hindered the migration of thymic αβT cells into the circulation and then into LNs, accompanied by the downregulation of KLF2, CCR7, and CD62L. Mechanistically, Med1 promotes Klf2 transcription by facilitating Foxo1 binding to the Klf2 enhancer. Furthermore, forced expression of Klf2 rescued Ccr7 and Sell expression, as well as αβT‐cell migration into LNs. Collectively, our study unveils a crucial role for Med1 in regulating the enhancer‐based Foxo1‐Klf2 transcriptional program and the migration of αβT cells into LNs, providing valuable insights into the molecular mechanisms underlying T‐cell migration.
Understanding the conversion of nitrogen compounds during ammonia electrooxidation: effect of current density, chloride concentration and pH on nitrate formation
Authors: Galvão Neanderson , Dutra Achilles Junqueira Bourdot , Bassin João Paulo 
Published: August 2024

AbstractBACKGROUNDAmmonia removal from wastewater by electrooxidation (EO) is an interesting approach because of its efficiency and easy maintenance and operation. In this process, ammonia is oxidized to nitrate or nitrogen gas. Ammonia conversion to the latter is desirable to remove nitrogen from the liquid; however, the influence of several operating parameters on nitrate generation has not been systematically evaluated. Therefore, this work aimed to investigate the effect of current density (200–800 A m−2), chloride concentration (0–10 000 mg L) and initial pH (5–9) on the electrooxidation of an ammonia‐containing solution and the associated generation of nitrate. For this purpose, a laboratory‐scale electrochemical reactor containing two Ti/RuO2 electrodes was used.RESULTSThe results indicated high ammonia removal efficiency – 98% within 150 min at 800 A m−2 and 97% within 240 min at 500 A m−2 – generating around 70 and 102 mg ‐N L−1 under these conditions, respectively. Increasing the chloride concentration from 5000 to 7500 mg L−1 reduces the electrolysis time needed to remove all ammonia from 180 to 150 min. However, with increasing initial chloride concentration, the amount of nitrate generated rose from 69.5 to 135.9 mg N L−1. On the other hand, in the test without chloride, nitrate generation was considerably lower (0–0.61 mg  L−1).CONCLUSIONThe higher the current density applied, the greater the ammonia removal by EO. Although current density influenced the ammonia oxidation rates, it did not directly affect nitrate formation. The lower the concentration of ammonia in the solution, the more significant was the fraction of nitrate generated. Most of the inlet ammonia was oxidized to nitrogen gas, nitrite was rapidly oxidized to nitrate and higher chloride concentrations enhanced ammonia oxidation. © 2024 Society of Chemical Industry (SCI).
Prevalence and impact of signs and symptoms of temporomandibular disorders in dental students and faculty
Authors: Larkin Nathan , Fricton Vincent , Sangalli Linda , Prodoehl Janey , Fricton James 
Published: August 2024

AbstractObjectivesTemporomandibular disorders (TMD) affect individuals undergoing increased stress, for example, dental professionals. No study to date has compared dental students and faculty in TMD manifestation. This cross‐sectional study compared prevalence, risk factors, and impact on daily activities of self‐reported TMD signs/symptoms between dental students and faculty at a predoctoral dental school, and between preclinical (first‐ to second‐year) and clinical (third‐ to fourth‐year) students.MethodsA REDCap survey was sent to dental students and faculty, assessing TMD signs/symptoms (TMD Symptom Questionnaire), pain intensity (Chronic Graded Pain Scale), jaw function (Jaw Function Limitation Scale), parafunctions (Oral Behavioral Checklist), previous TMD treatments, anxiety/depression symptoms (Patient Health Questionnaire), perceived stress (Perceived Stress Scale), and sleep quality (RU‐SATED Scale). Outcomes were compared between groups using chi‐square and t‐tests, adjusting for covariates with analyses of covariance (ANCOVA).ResultsData derived from N = 145 participants (N = 108 students, N = 37 faculty). Dental students reported significantly higher prevalence (90.1% vs. 75.7%, p = 0.020) and greater number of TMD signs/symptoms (5.3 ± 3.5 vs. 3.0 ± 2.7, p < 0.001) compared to faculty. Students reported significantly more parafunctional activities (p = 0.000), jaw‐strain episodes (prolonged mouth opening, p = 0.007), higher stress level (p = 0.008), and lower sleep quality (p = 0.002) than faculty. Difference in number of TMD signs/symptoms was maintained after adjusting for stress, sleep quality, and parafunctional/jaw‐strain activities. Clinical students utilized significantly more often evidence‐based TMD treatment compared to preclinical students.ConclusionsHigh prevalence of self‐reported TMD was observed among dental students and faculty, with students reporting higher prevalence and impairment despite working in the same environment. Findings underscore the importance of education on preventive measures early in dental training to address contributing factors and TMD management.
IDH2 mutation accelerates TPO‐induced myelofibrosis with enhanced S100a8/a9 and NFκB signaling in vivo
Authors: Lin Chien‐Chin , Yao Chi‐Yuan , Wang Yu‐Hung , Hsu Yueh‐Chwen , Yuan Chang‐Tsu , Chen Tsung‐Chih , Hsu Chia‐Lang , Lee Sze‐Hwei , Lee Jhih‐Yi , Shih Pin‐Tsen , Kao Chein‐Jun , Chuang Po‐Han , Kuo Yuan‐Yeh , Hou Hsin‐An , Chou Wen‐Chien , Tien Hwei‐Fang 
Published: July 2024

AbstractIntroductionIDH2 mutation is an unfavorable prognostic factor in patients with primary myelofibrosis (PMF) but its effect on myelofibrosis (MF) remains largely unclear.MethodsIn this study, we aimed to elucidate the roles of IDH2 mutation in the development and progression of MF by transcriptomic and molecular techniques using the Idh2R172K transgenic mice.ResultsWe found that thrombopoietin (TPO)‐overexpressed Idh2R172K (Idh2R172K + TPO) mice had accelerated progression to MF, compared with TPO‐overexpressed Idh2‐wild (WT + TPO) mice, showing activation of multiple inflammatory pathways, among which nuclear factor κB (NFκB) was the most significantly enhanced. Single‐cell transcriptomes of the marrow cells in early MF showed that S100a8/a9 expression was mainly confined to neutrophil progenitors in the WT + TPO mice, but highly expressed in several types of myeloid precursor cells, including the megakaryocyte progenitors in the Idh2R172K + TPO group. Furthermore, Idh2R172K mice at age of 18 months had larger spleens, increased S100a8/a9‐Tlr4 expression, and elevated serum S100a8/a9 levels compared with WT mice. PMF patients with IDH2 mutations had higher bone marrow plasma S100A8/A9 levels than those without IDH2 mutations.ConclusionOverall, our findings showed that IDH2 mutation induced proinflammatory effects, which further exacerbated MF, as evidenced by the increase in S100a8/a9 levels and NFκB hyperactivation in Idh2R172K + TPO mice.