bluuebunny/crossref_metadata_2025_split huggingface dataset:
F pocket flexibility influences the tapasin dependence of two differentially disease‐associated MHC Class I proteins
Authors: Abualrous Esam T. , Fritzsche Susanne , Hein Zeynep , Al‐Balushi Mohammed S. , Reinink Peter , Boyle Louise H. , Wellbrock Ursula , Antoniou Antony N. , Springer Sebastian 
Published: January 2015

The human MHC class I protein HLA‐B*27:05 is statistically associated with ankylosing spondylitis, unlike HLA‐B*27:09, which differs in a single amino acid in the F pocket of the peptide‐binding groove. To understand how this unique amino acid difference leads to a different behavior of the proteins in the cell, we have investigated the conformational stability of both proteins using a combination of in silico and experimental approaches. Here, we show that the binding site of B*27:05 is conformationally disordered in the absence of peptide due to a charge repulsion at the bottom of the F pocket. In agreement with this, B*27:05 requires the chaperone protein tapasin to a greater extent than the conformationally stable B*27:09 in order to remain structured and to bind peptide. Taken together, our data demonstrate a method to predict tapasin dependence and physiological behavior from the sequence and crystal structure of a particular class I allotype.Also watch the Video Abstract
Systematic purification of salt‐intolerant proteins by ion‐exchange chromatography: The example of human α‐galactosidase A
Authors: Baumann Pascal , Osberghaus Anna , Hubbuch Jürgen 
Published: January 2015

Chromatography is an essential tool for purifying biopharmaceutical products. Many processes are still developed based on traditional routines and empirical procedures. Product losses are mostly due to insufficient optimization of purification setups and product sensitivity to process conditions. In order to eliminate these shortcomings, a systematic strategy for the setup of ion‐exchange chromatography is presented, which considers both product stability as well as operational conditions. The stages—a hybrid approach combining high‐throughput screening and analytical small‐scale chromatography—are as follows: (1) pH stability (short‐term); (2) pH stability (long‐term), followed by a screening of additives to enhance protein stability, if required; (3) analytical pH gradient chromatography for evaluation of the operational pH window; and (4) salt stability (long‐term) in the operational pH window determined. The efficiency and straightforwardness of the strategy were shown in a case study on capturing the human α‐galactosidase A enzyme. Following the above procedure, the enzyme was found to be salt‐unstable; a purification factor of 13.2, a concentration factor of 4, and an overall yield of 84.3% were achieved. The applied strategy allowed for a quick establishment of a dedicated capture step at low salt concentrations under stable conditions by well‐chosen prior screening experiments.
Cyclic Dehydrogenation–(Re)Hydrogenation with Hydrogen‐Storage Materials: An Overview
Authors: Chamoun Rita , Demirci Umit B. , Miele Philippe 
Published: January 2015

AbstractHydrogen‐storage materials (HSMs) have especially revolutionized the field of “hydrogen storage” by offering a relatively safe storage alternative and high gravimetric hydrogen density. However, a critical challenge is storage reversibility. This is the central tenet of the present article, which discusses ways to close the hydrogen cycle with solid‐ and liquid‐phase HSMs. A distinction between the different HSMs is made on the basis of the approach under which they can be rehydrogenated; this led to the proposition of distribution in three categories: one, materials allowing reversible storage of H2; two, hydrogen carriers for which the byproducts must be recycled; three, sustainable hydrogen carriers with which the hydrogen cycle is considered as being byproduct free. From our analysis, supported by many examples, it seems that the hydrogen cycle can be closed with all of the HSMs discussed herein and that the dehydrogenation/rehydrogenation paths depend on their nature.
Issues and Challenges for Bulk‐Type All‐Solid‐State Rechargeable Lithium Batteries using Sulfide Solid Electrolytes
Authors: Jung Yoon Seok , Oh Dae Yang , Nam Young Jin , Park Kern Ho 
Published: January 2015

AbstractAs lithium rechargeable batteries are considered a potential candidate for large‐scale energy storage applications in devices such as electric vehicles (EVs) and smart grids, their safety has become of prime concern. This calls for the need to replace the flammable organic liquid electrolyte (LE) with an inorganic solid electrolyte (SE), and thus, develop bulk‐type all‐solid‐state lithium batteries (ASLBs), fabricated using a scalable process. Sulfide SEs are considered the most competitive candidate owing to their high conductivity at room temperature (10−3–10−2 S cm−1), which is comparable to that of LEs, and their ductility, which enables the fabrication of ASLBs simply using cold pressing. In the present review, issues and challenges to be faced for the fabrication of bulk‐type ASLBs using sulfide SEs are presented and discussed, with a special focus on the development of SEs, compatibility of the electrode materials with SEs, and structure of the composite electrodes. Recent progress made with the aim of addressing the aforementioned issues and challenges is also presented, to provide an outlook on the future of SEs and ASLBs.
Recent Development on Anodes for Na‐Ion Batteries
Authors: Bommier Clement , Ji Xiulei 
Published: January 2015

AbstractNew discoveries in anode materials for sodium ion batteries (NIBs) are highly necessary to achieve the goals of widespread applications, such as electric vehicles (EVs) and grid‐level energy storage. Carbon‐based materials are critical for this task as they are inexpensive, abundant, and versatile. They contain a plethora of structures and morphologies, ranging from highly ordered graphite or nanotubes to highly disordered amorphous carbon, thus making them very attractive for electrochemical energy storage. This review attempts to cover past and recent progress in the development of carbon‐based anode materials for NIBs. To give a larger context, the article will briefly cover other anode materials for NIBs as well. The aim of this paper is to provide a timely update for researchers currently involved in the respective fields or to serve as a starting point for individuals who would like to gain a greater knowledge of new NIB anode materials.
Bisphenol A exposure induces metabolic disorders and enhances atherosclerosis in hyperlipidemic rabbits
Authors: Fang Chao , Ning Bo , Waqar Ahmed Bilal , Niimi Manabu , Li Shen , Satoh Kaneo , Shiomi Masashi , Ye Ting , Dong Sijun , Fan Jianglin 
Published: January 2015

ABSTRACTBisphenol A (BPA) is an artificial environmental endocrine disrupter. Excess exposure to BPA may induce many disorders in the metabolism and cardiovascular system. However, the underlying toxicological mechanisms remain largely unknown. In this study, we administered genetically hyperlipidemic Watanabe heritable hyperlipidemic (WHHL‐MI) rabbits (male, 14 week old), which have more common features with humans than the mouse and rat especially in the metabolism and cardiovascular system, with BPA at 40 mg kg–1 day–1 for 8 weeks by gavage and compared their plasma lipids, glucose and insulin response with those of the vehicle group. All of the rabbits were sacrificed, and their pancreas, liver, adipose tissue, heart and aorta were analyzed using histological and morphometric methods. Furthermore, we treated human hepatoma HepG2 cells and human umbilical cord vein endothelial cells (HUVECs), with different doses of BPA based on the serum BPA levels in the WHHL rabbits for 6 h to investigate the possible molecular mechanisms. Our results showed that BPA‐treated rabbits showed insulin resistance, prominent adipose accumulation and hepatic steatosis. Additionally, BPA exposure also caused myocardial injury and enhanced the development of atherosclerosis in the aortic arch with increased macrophage number (86%) and advanced lesion areas (69%). Increased expression of inflammatory genes found in the liver of BPA‐treated rabbits along with the up‐regulation of ER stress, lipid and glucose homeostasis and inflammatory genes in the cultured HepG2 cells and HUVECs suggest that BPA may induce metabolic disorders and enhance atherosclerosis through regulating above molecular pathways in the liver and endothelium. Copyright © 2015 John Wiley & Sons, Ltd.
An empirical I‐V model of tunneling real‐space transfer transistors for monostable–bistable transition logic element application
Authors: Bai Fan , Li Liping 
Published: January 2015

SummaryWe proposed an empirical I‐V model to represent the negative differential resistance (NDR) regime of fabricated tunneling real‐space transfer transistors (TRSTTs). For TRSTTs to have great potential in monostable–bistable transition logic element (MOBILE) design, our model is able to accurately reproduce the NDR regime including gate‐source‐bias‐controlled NDR values and modulated peak to valley drain current ratios. The modeled I‐V curves, tranconductances, and NDRs with multiple gate biases are in good agreement with measured data. The key parameters in the model have clear physical meanings, and the value of these parameters is easy to be extracted directly from the test I‐V curves. The model is used to simulate a practical MOBILE, and excellent agreement between the simulated and measured data was found. Copyright © 2015 John Wiley & Sons, Ltd.
Evaluation of Muscodor suthepensis strain CMU‐Cib462 as a postharvest biofumigant for tangerine fruit rot caused by Penicillium digitatum
Authors: Suwannarach Nakarin , Bussaban Boonsom , Nuangmek Wipornpan , Pithakpol Wasna , Jirawattanakul Bantoon , Matsui Kenji , Lumyong Saisamorn 
Published: January 2015

AbstractBACKGROUNDThis study investigated both the in vitro and in vivo biofumigant ability of the endophytic fungus Muscodor suthepensis CMU‐Cib462 to control Penicillium digitatum, the main cause of tangerine fruit rot.RESULTSVolatile compounds from M. suthepensis inhibited mycelial growth of the pathogen. The most abundant compound was 2‐methylpropanoic acid, followed by 3‐methylbutan‐1‐ol. They showed median effective doses (ED50) on P. digitatum growth of 74.91 ± 0.73 and 250.29 ± 0.29 µL L−1 airspace respectively. Rye grain was found to be a suitable solid medium for M. suthepensis inoculum production. The results indicated that mycofumigation with a 30 g rye grain culture of M. suthepensis for 12 h controlled tangerine fruit rot. The percentage weight loss and soluble solids concentration of fumigated tangerines were similar to those of non‐infected and non‐fumigated fruits.CONCLUSIONMuscodor suthepensis has potential as a biofumigant for controlling postharvest disease of tangerine fruit. © 2015 Society of Chemical Industry
Development of delayed bitterness and effect of harvest date in stored juice from two complex citrus hybrids
Authors: Raithore Smita , Dea Sharon , McCollum Greg , Manthey John A , Bai Jinhe , Leclair Clotilde , Hijaz Faraj , Narciso Jan A , Baldwin Elizabeth A , Plotto Anne 
Published: January 2015

AbstractBACKGROUNDMandarins and mandarin hybrids have excellent flavor and color attributes, making them good candidates for consumption as fresh fruit. When processed into juice, however, they are less palatable, as they develop delayed bitterness when stored for a period of time. In this study the kinetics of delayed bitterness in two citrus mandarin hybrid siblings, ‘Ambersweet’ and USDA 1‐105‐106, was explored by sensory and instrumental analyses. In addition to the bitter limonoids, other quality factors (i.e. sugars, acids, pH, soluble solids content (SSC), titratable acidity (TA) and the ratio SSC/TA) were also measured.RESULTSThe two citrus hybrid siblings had different chemical profiles, which were perceived by taste panels. USDA 1‐105‐106 developed delayed bitterness when the juice was stored for more than 4 h, similar to juice from ‘Navel’ oranges, but ‘Ambersweet’ did not. Bitterness in ‘Ambersweet’ was more affected by harvest maturity, as juice from earlier harvest had lower SSC but higher TA and bitter limonoids.CONCLUSIONSince juice of USDA 1‐105‐106 shows delayed bitterness when stored for more than 4 h, this cultivar is not suitable for juice processing. Our finding that siblings can differ in chemical and sensory properties emphasize the importance of post‐processing storage studies before releasing cultivars for juice. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.
Synthesis, Photophysical, and Morphological Properties of Azomethine‐Persylilated α‐Cyclodextrin Main‐Chain Polyrotaxane
Authors: Farcas Aurica , Resmerita Ana‐Maria , Aubert Pierre‐Henri , Ghosh Indrajit , Cantin Sophie , Nau Werner M. 
Published: January 2015

The synthesis of 2‐rotaxane (3•TMS‐αCD) through a condensation reaction between 3,5–diamino‐1,2,4‐triazole encapsulated into hexakis(2,3,6‐trimethylsilyl) α‐cyclodextrin cavity (TMS‐αCD) and 1‐pyrenecarboxaldehyde is reported. The oxidative coupling of 3•TMS‐αCD afforded then pyrene‐triazole/TMS‐αCD PAMs polyrotaxane (4•TMS‐αCD) azomethine polyrotaxane. The optical, electrochemical, morphological, surface‐free energies, as well as transport properties of 3•TMS‐αCD and its corresponding 4•TMS‐αCD polyrotaxane have been investigated and compared with those of the reference polymer pyrene‐triazole azomethine. The polyrotaxane is soluble in toluene/dimethylformamide (DMF) 1:1, v/v, mixture and displays useful levels of thermal stability and higher fluorescence quantum yield (ΦPL) in DMF solutions. ΦPL improvement is further reflected in the fluorescence lifetime (τF), significantly longer than that of the starting monomer 3•TMS‐αCD (7.8 vs 0.89 ns). In addition, a smoother surface with the smaller grains uniformly distributed on the surface, as well as lower surface‐free energy, combined with energy gap (3.32 vs 3.76 eV) represent noticeable advantages of azomethine backbones encapsulation by TMS‐αCD.
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Super‐resolution reconstruction of diffusion parameters from diffusion‐weighted images with different slice orientations
Authors: Van Steenkiste Gwendolyn , Jeurissen Ben , Veraart Jelle , den Dekker Arnold J. , Parizel Paul M. , Poot Dirk H. J. , Sijbers Jan 
Published: January 2015

PurposeDiffusion MRI is hampered by long acquisition times, low spatial resolution, and a low signal‐to‐noise ratio. Recently, methods have been proposed to improve the trade‐off between spatial resolution, signal‐to‐noise ratio, and acquisition time of diffusion‐weighted images via super‐resolution reconstruction (SRR) techniques. However, during the reconstruction, these SRR methods neglect the q‐space relation between the different diffusion‐weighted images.MethodAn SRR method that includes a diffusion model and directly reconstructs high resolution diffusion parameters from a set of low resolution diffusion‐weighted images was proposed. Our method allows an arbitrary combination of diffusion gradient directions and slice orientations for the low resolution diffusion‐weighted images, optimally samples the q‐ and k‐space, and performs motion correction with b‐matrix rotation.ResultsExperiments with synthetic data and in vivo human brain data show an increase of spatial resolution of the diffusion parameters, while preserving a high signal‐to‐noise ratio and low scan time. Moreover, the proposed SRR method outperforms the previous methods in terms of the root‐mean‐square error.ConclusionThe proposed SRR method substantially increases the spatial resolution of MRI that can be obtained in a clinically feasible scan time. Magn Reson Med 75:181–195, 2016. © 2015 Wiley Periodicals, Inc.
Sheep, Cattle, and Specialization: New Zooarchaeological Perspectives on the Taosi Longshan
Authors: Brunson K. , He N. , Dai X. 
Published: January 2015

AbstractIn this paper, we present a zooarchaeological analysis of the Longshan period sites of Taosi and Zhoujiazhuang (ca. 2300–1900 cal. BC) in southern Shanxi Province, China. We compare the faunal record at both sites in terms of the proportions of wild and domestic taxa; the slaughter patterns for the main domesticates; the types of bones used to produce utilitarian and decorative bone artifacts; and the types of bones used for ritual oracle bone divination. Differences in the faunal records at Taosi and Zhoujiazhuang provide insights into the connections between specialization and early urbanism. Our research also provides clues about how sheep and cattle pastoralism was initially adopted in the Yellow River Valley during the late 3rd millennium BC. Copyright © 2015 John Wiley & Sons, Ltd.
Isotopic Evidence for Marine Consumption and Mobility in the Atacama Desert (Quillagua, Northern Chile)
Authors: Santana‐Sagredo F. , Hubbe M. , Uribe M. 
Published: January 2015

AbstractArchaeological research in the Atacama Desert has recovered evidence of considerable cultural variability. This variability seems to have increased during the Late Intermediate Period (AD 950–1400). The oasis of Quillagua, located at the margins of the Loa River in northern Chile, between the Andes and the coast (70 km from the Pacific Ocean), has shown important evidence regarding this cultural diversity. The variety in the archaeological evidence found at Quillagua has been interpreted as the result of two different cultural influences at the oasis: the Pica‐Tarapacá who occupied the coastal and inland regions to the north of Quillagua and the Atacama who occupied the oasis and fertile areas southeast of Quillagua. Here, we present the results of stable carbon, nitrogen and oxygen isotope analyses of 23 individuals recovered from the Cementerio Oriente in Quillagua, in order to test whether the observed cultural variability is also reflected in diet and mobility patterns. Results from carbon and nitrogen isotope measurements indicate the importance of marine protein, as well as a contribution of maize in the diet of some individuals. Four individuals show low δ18O values, suggesting a possible highland or non‐local origin, whereas values for the remaining individuals are consistent with lowland populations. Together, the results support the idea that the Quillagua oasis represented an important site of interaction between the Tarapacá and Atacama cultures, with close contacts with the coast but also with the presence of individuals from the highlands. Copyright © 2015 John Wiley & Sons, Ltd.
Health Patterns of the Etruscan Population (6th–3rd Centuries bc) in Northern Italy: The Case of Spina
Authors: Manzon V. S. , Gualdi‐Russo E. 
Published: January 2015

AbstractA sample of 303 skeletons from the Etruscan necropolis of Spina (Ferrara, Italy, 6th–3rd centuries bc) was examined for paleopathological lesions in order to assess the general health, diseases and quality of life of this Iron Age population. The observed pathologies included porotic hyperostosis, specific and aspecific infections, metabolic and endocrine disorders, tumours and osteoarthritis. A total of 46.7% of adults and 7.7% of subadults showed at least one pathological lesion. Statistical comparisons were made between sexes and adult‐age classes (20–35 years and >35 years) to analyse the prevalence of the lesions. The analysis of porotic hyperostosis and osteoperiostitis allowed to determine the general health status of this group, and the analysis of osteoarthritis allowed to hypothesise a gender division of labour. The results suggest a relatively high‐life expectancy for the time as well as good health and quality of life. The few specific infections and metabolic disorders indicate fairly good nutrition, while the high frequency of porotic hyperostosis can be partly linked to some type of hereditary anaemia. Copyright © 2015 John Wiley & Sons, Ltd.
Degradation of external quantum efficiency of AlGaN UV LEDs grown by hydride vapor phase epitaxy
Authors: Shmidt Natalia , Usikov Alexander , Shabunina Eugenia , Chernyakov Anton , Sakharov Alexey , Kurin Sergey , Antipov Andrei , Barash Iosif , Roenkov Alexander , Helava Heikki , Makarov Yuri 
Published: January 2015

AbstractA comparative study of the degradation of HVPE‐grown 360 nm AlGaN/GaN UV and commercially available InGaN/GaN blue LED chips was performed. The common feature of the degradation of these two types of LEDs was found to be the increase of the conductivity of shunt paths under current injection. The paths (shunts) are localized in the extended defects system (EDS). It is proposed that the conductivity increase is due to defect formation under multiphonon carriers recombination in a part of the EDS enriched by Ga or In atoms. This process is accompanied by a local overheating and migration of Ga or In atoms. To increase the lifetime of the AlGaN/GaN UV LEDs to more than 2000 h it is necessary to improve their nano‐structural and nanoscale AlGaN composition ordering. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Log‐normal distribution based Ensemble Model Output Statistics models for probabilistic wind‐speed forecasting
Authors: Baran Sándor , Lerch Sebastian 
Published: January 2015

Ensembles of forecasts are obtained from multiple runs of numerical weather forecasting models with different initial conditions and typically employed to account for forecast uncertainties. However, biases and dispersion errors often occur in forecast ensembles: they are usually underdispersive and uncalibrated and require statistical post‐processing. We present an Ensemble Model Output Statistics (EMOS) method for calibration of wind‐speed forecasts based on the log‐normal (LN) distribution and we also show a regime‐switching extension of the model, which combines the previously studied truncated normal (TN) distribution with the LN.Both models are applied to wind‐speed forecasts of the eight‐member University of Washington mesoscale ensemble, the 50 member European Centre for Medium‐Range Weather Forecasts (ECMWF) ensemble and the 11 member Aire Limitée Adaptation dynamique Développement International‐Hungary Ensemble Prediction System (ALADIN‐HUNEPS) ensemble of the Hungarian Meteorological Service; their predictive performance is compared with that of the TN and general extreme value (GEV) distribution based EMOS methods and the TN–GEV mixture model. The results indicate improved calibration of probabilistic forecasts and accuracy of point forecasts in comparison with the raw ensemble and climatological forecasts. Further, the TN–LN mixture model outperforms the traditional TN method and its predictive performance is able to keep up with models utilizing the GEV distribution without assigning mass to negative values.
Bladder rupture – a rare complication of extraperitoneal balloon dissection during robot‐assisted radical prostatectomy
Authors: Agrawal Vineet , Joseph Jean 
Published: January 2015

AbstractIntroductionBalloon dissection to create the extraperitoneal space to facilitate laparoscopic procedures is generally a safe step. However, complications can include vascular and visceral injuries. Bladder injury during balloon dilation of extraperitoneal space has previously been described during herniorraphy. This is the first reported case of bladder injury during balloon dilation of the extraperitoneal space during robot‐assisted radical prostatectomy.MethodsWe report transection of bladder at the prostato‐vesical junction, during balloon dilation of extraperitoneal space, in a 66‐year‐old man undergoing extraperitoneal robot‐assisted radical prostatectomy.ResultsThe patient had an uneventful procedure due to immediate recognition of this intra‐operative complication. At 6‐month follow‐up, he reports complete return of urinary continence and is free from biochemical recurrence from prostate cancer.DiscussionAlbeit uncommon, balloon‐related complications can occur. Surgeons performing extraperitoneal laparoscopic procedures should be aware of the range of potential complications specifically related to balloon dilation of extraperitoneal space. With experience, these complications can be mitigated. Copyright © 2015 John Wiley & Sons, Ltd.