bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Characteristics and Management of Periesophageal Vagal Nerve Injury Complicating Left Atrial Ablation of Atrial Fibrillation: Lessons from Eleven Cases
Authors: KUWAHARA TAISHI , TAKAHASHI ATSUSHI , TAKAHASHI YOSHIHIDE , KOBORI ATUSHI , MIYAZAKI SHINSUKE , TAKEI ASUMI , FUJINO TADASHI , OKUBO KENJI , TAKAGI KATSUMASA , FUJII AKIRA , TAKIGAWA MASATERU , WATARI YUJI , HIKITA HIROYUKI , SATO AKIRA , AONUMA KAZUTAKA 
Published: March 2013

Postablation Gastroparesis Features and TreatmentIntroductionThis study aimed to elucidate the clinical characteristics and management of periesophageal vagal nerve injury complicating the ablation of atrial fibrillation (AF).Methods and ResultsA total of 3,695 patients with drug‐resistant AF underwent extensive pulmonary vein isolation at our institution. Either a nonirrigated or an irrigated ablation catheter was employed, with radiofrequency power of 25–40 W. Esophageal temperature was monitored in 3,538 patients: when the esophageal temperature reached 42°C radiofrequency delivery was stopped. A total of 11 patients (60 ± 11 years, 10 males) were diagnosed as having a periesophageal vagal nerve injury after the AF ablation. Symptoms included nausea, vomiting, bloating, constipation, and gastric pain, which occurred within 72 hours after the procedure. Gastrointestinal fluoroscopy and/or endoscopy revealed gastric hypomotility (10 patients) and pyloric spasm (1 patient). Intravenous erythromycin (3 mg/kg every 8 hours) was effective in relieving symptoms in 5 patients, and the patient with pyloric spasm underwent esophagojejunal anstomosis. Eight patients almost fully recovered within 40 days; however, 3 patients suffered from severe symptoms for 3–12 months. This complication occurred in 4 of the 157 patients (2.5%) who did not have esophageal temperature monitoring, and 7 of the 3,538 (0.2%) who did (P = 0.0007). The 3 patients with persistent severe symptoms received no esophageal temperature monitoring.ConclusionThe clinical course and severity of the periesophageal vagal nerve injury varied, but most patients finally recovered with conservative treatment. Radiofrequency delivery under esophageal temperature monitoring might reduce both the incidence and the severity of this complication.
Beneficial effect of calcium channel blockers on home blood pressure variability in the morning in patients with type 2 diabetes
Authors: Ushigome Emi , Fukui Michiaki , Hamaguchi Masahide , Tanaka Toru , Atsuta Haruhiko , Ohnishi Masayoshi , Oda Yohei , Yamazaki Masahiro , Hasegawa Goji , Nakamura Naoto 
Published: March 2013

AbstractAims/IntroductionRecent studies have shown the association between blood pressure variability and cardiovascular events. The present study was designed to investigate the relationship between antihypertensive drug class and home blood pressure variability in patients with type 2 diabetes.Materials and MethodsWe compared home blood pressure variability among patients treated with calcium channel blockers (n = 44), with angiotensin II receptor blockers and/or angiotensin‐converting enzyme inhibitors (n = 159), and with calcium channel blockers combined with angiotensin II receptor blockers and/or angiotensin‐converting enzyme inhibitors (n = 183). Next, we analyzed the effect of calcium channel blockers on morning blood pressure variability using multiple linear regression analysis.ResultsCoefficient variation of morning systolic blood pressure in patients treated with calcium channel blockers was significantly lower than that in patients treated with angiotensin II receptor blockers and/or angiotensin‐converting enzyme inhibitors (P = 0.036). Multivariate linear regression analyses showed that treatment with calcium channel blockers was significantly correlated with coefficient variation of morning systolic blood pressure (β = −0.264, P = 0.001).ConclusionsThe present study implies a possibility for validity on selecting calcium channel blockers in hypertensive patients with type 2 diabetes to reduce home blood pressure variability.
MC1R major variants are a risk factor of sleep lines in Caucasian women
Authors: Jdid R. , Ezzedine K. , Latreille J. , Galan P. , Hercberg S. , Malvy D. , Tschachler E. , Guinot C. 
Published: March 2013

AbstractBackgroundSleep lines are caused by individual's sleeping positions and should be differentiated from expression wrinkles.ObjectiveThe aim of the study was to investigate possible risk factors for sleep lines on a sizeable sample of middle‐aged Caucasian women.MethodsThis study involved a sample of 542 French middle‐aged women (44 to 70 years old) from Paris area. Three standardized facial photographs (face and profiles) were examined independently by two dermatologists allowing the identification of sleep lines and the evaluation of the severity of several facial skin features. Possible impacts of MC1R gene polymorphisms were tested using logistic regression models.ResultsSixty women (11%) had facial sleep lines and showed generally more than one sleep line. The sleep lines were often located on the forehead, along the nose, on the cheeks and under the eyes, and more rarely on the chin. As expected, the sleep lines were associated with age, and the women with sleep lines showed also more severe signs of skin ageing. After adjustment on possible confounders, the presence of two major diminished function variants of the MC1R gene was identified as a strong risk factor for sleep lines [adjusted odds ratios (AOR) (95% CI): 8.25 (2.62–25.97)].Discussion/ConclusionThe data in the literature are scarce and this study is the first to be conducted on a sizeable sample of women. Our results suggest that genetic variations of MC1R are important determinants of the development of sleep lines.
Rosacea under the microscope: characteristic histological findings
Authors: Cribier B. 
Published: March 2013

AbstractRosacea is a common facial dermatosis that is seldom biopsied; thus, histological aspects have not been well described. Biopsies are generally performed in the presence of atypical symptoms (e.g. granulomas). Differential diagnosis with sarcoidosis, lupus miliaris or lupus erythematosus is another indication for biopsy. There are few published studies addressing the microscopic aspects of rosacea and describing the histological and immunohistochemical features of this disease. While some textbooks consider the microscopic signs of rosacea to be non‐diagnostic, experienced dermatopathologists are generally able to make the diagnosis via histology. This article discusses the specific combinations of histological features that are highly suggestive of rosacea.
Pimecrolimus vs. tacrolimus for the topical treatment of unresponsive oral erosive lichen planus: a 8 week randomized double‐blind controlled study
Authors: Arduino P.G. , Carbone M. , Della Ferrera F. , Elia, A. , Conrotto D. , Gambino A. , Comba A. , Calogiuri P.L. , Broccoletti R. 
Published: March 2013

AbstractBackgroundOral lichen planus (OLP) is a chronic inflammatory disease, affecting nearly 1–2% of the population; Proposed therapies are usually symptomatic and numerous drugs have been used, but recently, it has been published that there is insufficient evidence to support the effectiveness of any specific treatment as being superior. To the best of our knowledge, direct evaluation of the efficacy of topically applied pimecrolimus and tacrolimus in the treatment of atrophic‐erosive OLP, refractory to topical steroids, is still lacking.ObjectivesTo assess the efficacy and safety of topical calcineurin inhibitors for unresponsive OLP. An 8 week randomized, double‐blind controlled trial, followed by a 6 month follow‐up period. Patients were treated with either pimecrolimus 1% cream or tacrolimus 0.1% ointment, both mixed with an equivalent amount of 4% hydroxyethyl cellulose gel. The medications were to be applied twice daily for 2 months. Each patient was examined at the beginning of therapy, and then every 2 weeks during the treatment and every 3 months of follow‐up. Main outcome measures were: (i) to compare the effectiveness of topically applied pimecrolimus and tacrolimus; (ii) to evaluate which is more cost‐effective; (iii) to determine which drug is faster in reducing signs and symptoms and (iv) which gives the longest remission.ResultsThirty patients were involved in the study. Both drugs were effective at inducing clinical improvement, with no statistical difference. Pimecrolimus creams revealed a significantly better stability of the therapeutic effectiveness (P = 0.031).ConclusionBoth medications would currently appear to be a treatment of choice for patients with unresponsive atrophic‐erosive OLP. Pimecrolimus seemed to be more effective in providing long‐term resolution of signs and symptoms. Future efforts are however needed to obtain more objective evidence of the benefit of these medications in the treatment of immunologically mediated oral mucosal lesion.
Susceptibility of Morganella morganii to Various Environmental Stresses after Cold and Heat Shock Treatments
Authors: Zarei Mehdi , Maktabi Siavash , Khezrzadeh Marjan , Jamnejad Amirhesam 
Published: March 2013

AbstractMorganella morganii was subjected to cold shock treatment at 10C for 2 h or heat shock treatment at 42C for 45 min. The effect of these shock treatments on the viability of M. morganii under adverse conditions was investigated. Viability of M. morganii cells during storage at 4C was slightly affected by cold shock treatment under the conditions tested (P > 0.05). However, a significant increase in the viable population of the cold‐shocked cells was found in the subsequent challenge of −18C, as compared with the nonshocked cells (P < 0.05). In addition, cold‐shocked cells became more susceptible to high incubation temperature (P < 0.05). Nevertheless, cold shock treatment did not alter the susceptibility of this bacterium to 1.0 mM H2O2, 20% NaCl and low pH (pH, 3.5). On the contrary, heat shock treatment increased the susceptibility of M. morganii to low incubation temperatures and H2O2, while made the cells more resistant to high temperature. As in the cold shock treatment, heat shock treatment under the conditions tested did not affect the susceptibility of M. morganii to NaCl and low pH.Practical ApplicationsThese findings may be of significant importance with regard to food safety because cooling chains and heating regimes do exist in the food industry, which could potentially induce a cold or heat shock phenomenon.
Protein kinase Cβ is a modulator of the dopamine D2 autoreceptor‐activated trafficking of the dopamine transporter
Authors: Chen Rong , Daining Conor P. , Sun Haiguo , Fraser Rheaclare , Stokes Stephanie L. , Leitges Michael , Gnegy Margaret E. 
Published: March 2013

AbstractThe strength and duration of extracellular dopamine concentrations are regulated by the presynaptic dopamine transporter (DAT) and dopamine D2 autoreceptors (D2autoRs). There is a functional interaction between these two proteins. Activation of D2autoRs increases DAT trafficking to the surface whereas disruption of this interaction compromises activities of both proteins and alters dopaminergic transmission. Previously we reported that DAT expression and activity are subject to modulation by protein kinase Cβ (PKCβ). Here, we further demonstrate that PKCβ is integral for the interaction between DAT and D2autoR. Inhibition or absence of PKCβ abolished the communication between DAT and D2autoR. In mouse striatal synaptosomes and transfected N2A cells, the D2autoR‐stimulated membrane insertion of DAT was abolished by PKCβ inhibition. Moreover, D2autoR‐stimulated DAT trafficking is mediated by a PKCβ‐extracellular signal‐regulated kinase signaling cascade where PKCβ is upstream of extracellular signal‐regulated kinase. The increased surface DAT expression upon D2autoR activation resulted from enhanced DAT recycling as opposed to reduced internalization. Further, PKCβ promoted accelerated DAT recycling. Our study demonstrates that PKCβ critically regulates D2autoR‐activated DAT trafficking and dopaminergic signaling. PKCβ is a potential drug target for correcting abnormal extracellular dopamine levels in diseases such as drug addiction and schizophrenia.
Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats
Authors: Ozdemir H. , Toker H. , Balcı H. , Ozer H. 
Published: March 2013

AimThe purpose of this study was to analyze histologically the effect of ozone therapy in combination with autogenous bone graft on bone healing in rat calvaria.MethodsCritical size defects were created in calvaria of 27 male Wistar rats. The animals were divided into three groups of nine animals each: autogenous bone graft group (n = 9); autogenous bone graft with ozone therapy group (80%, 30 s 3 d for 2 wk, n = 9); non‐treatment (control) group (n = 9). Animals were killed after 8 wk. Histomorphometric assessments, using image analysis software, and histological analyses were performed. Primary outcome was total bone area. Secondary outcomes (osteoblast number, new bone formation) were also measured.ResultsHistomorphometrically, the total bone area in the autogenous bone graft with ozone therapy group (9.3 ± 2.2) were significantly higher than that of the autogenous bone graft group (5.1 ± 1.8) (p < 0.05). Also, the ozone therapy group significantly increased the percentage of total bone area compared to the autogenous bone graft group (p < 0.05). The osteoblast number significantly increased in the autogenous bone graft with the ozone therapy group (58 ± 12.3) compared to the autogenous bone graft group (9.3 ± 3.5) (p < 0.05). Also, it was observed that autogenous bone graft with ozone therapy group showed significant new bone formation when compared to the autogenous bone graft group (p < 0.05).ConclusionOzone therapy enhances new bone formation by autogenous bone graft in the rat calvarial defect model.
STIR muscle hyperintensity in the cervical muscles associated with inflammatory spinal cord disease of unknown origin
Authors: Eminaga S. , Cherubini G. B. , Villiers E. , Targett M. , Caine A. 
Published: March 2013

ObjectivesTo assess the relation of a distinctive pattern of short tau inversion recovery muscle hyperintensity with inflammatory cerebrospinal fluid result in dogs.MethodsAll dogs that had a short tau inversion recovery sequence performed in addition to other magnetic resonance sequences of the cervical spine and concurrent cerebrospinal fluid evaluation during the study period were included. All magnetic resonance studies were anonymised and reviewed by a board certified radiologist and board certified neurologist. A board certified pathologist examined the cerebrospinal fluid and the results were reviewed.ResultsForty‐nine cases fulfilled the inclusion criteria. Repeatable patterns of short tau inversion recovery hyperintensity were identified in 20 dogs. The clinical diagnosis in all these 20 cases was of meningoencephalomyelitis of unknown origin. This diagnosis was confirmed by inflammatory cerebrospinal fluid changes in 18 and suspected from clinical presentation and response to therapy in the remaining 2.Clinical SignificanceIn this study, the short tau inversion recovery changes identified were restricted to cases with inflammatory spinal cord disease. The short tau inversion recovery change had a sensitivity of 78%, and a specificity of 92% in predicting inflammatory cerebrospinal fluid, suggesting that short tau inversion recovery sequences are a useful addition to the investigation of suspected inflammatory spinal cord disease.
Characterisation of changes in the haemostasis system in dogs with thrombosis
Authors: Bauer N. , Moritz A. 
Published: March 2013

ObjectiveTo prospectively assess (2008 to 2009) all phases of coagulation including thromboelastography in dogs with thrombosis to determine if thrombosis is associated with hypercoagulable states.MethodsCoagulation reaction in dogs with thrombosis (n = 7) diagnosed by diagnostic imaging or histopathology was compared with 56 control dogs. Dogs pretreated with antiplatelet and anticoagulation drugs were excluded. Thromboelastographic G‐values >10 · 3 Kdyn/cm2 were used to define a hypercoagulable state.ResultsCompared with the controls, there was a significantly higher mean platelet component indicating lower platelet activation status (17 · 99 ±1 · 36 versus 20 · 48 ±2 · 04 g/dL, P = 0 · 0004), increased thromboelastographic G‐value (6 · 4 ±1 · 6 versus 13 · 1 ±6 · 2 Kdyn/cm2, P = 0 · 0029), activated partial thromboplastin time, fibrinogen, D‐dimers (all: P < 0 · 0001) as well as decreased antithrombin (P = 0 · 0049), factor VIII (P < 0 · 0001), protein C (P < 0 · 0001), protein S (P = 0 · 0373) and activated protein C‐ratio (P = 0 · 0013). On the basis of thromboelastographic G‐value, three of six thromboelastographic tracings were classified as normocoagulable and three as hypercoagulable.Clinical RelevanceIn dogs with thrombosis, both normo‐ and hypercoagulable states are present and activated protein C resistance is common.
Foetal echoencephalography and Doppler ultrasonography of the middle cerebral artery in canine foetuses
Authors: Feliciano M. A. R. , Nepomuceno A. C. , Crivalero R. M. , Oliveira M. E. F. , Coutinho L. N. , Vicente W. R. R. 
Published: March 2013

ObjectivesThis study aimed to evaluate the characteristics of the brain and vascular indicesof the middle cerebral artery of canine foetuses.MethodsTwenty‐five bitches were selected. Tissue development, echogenicity, echotexture and brain echobiometric data were studied, and the major structures were identified between the 5th and 8th gestational weeks. The area and volume of the brain mass (BMA and BMV), cranial area and volume (AC and VC), brain mass index (BMI) and brain volume index (BVI) were determined. A single ultrasound examination was performed during each studied week (6th, 7th and 8th). Doppler ultrasonography was performed to assess the maximum and minimum velocity, resistance and pulsatility index of middle cerebral artery of the foetuses.ResultsEchoencephalography was performed to evaluate the morphological characteristics of the central nervous system. Cerebral echobiometry indicated an increase in area and volume of the hemispheres and cranium (P<0·001) but no changes in BMI or BVI over the gestational period studied. Doppler ultrasonography identified increases in peak systolic velocity (P=0·0188) and end diastolic velocity (P=0·0274) and decreases in resistance index (P=0·0002) and pulsatility index (P<0·001).Clinical SignificanceEchoencephalography and spectral Doppler ultrasonography of the middle cerebral artery in canine foetuses might be a useful technique for prenatal care.
Sympatric diversification vs. immigration: deciphering host‐plant specialization in a polyphagous insect, the stolbur phytoplasma vector Hyalesthes obsoletus (Cixiidae)
Authors: Imo Miriam , Maixner Michael , Johannesen Jes 
Published: March 2013

AbstractThe epidemiology of vector transmitted plant diseases is highly influenced by dispersal and the host‐plant range of the vector. Widening the vector's host range may increase transmission potential, whereas specialization may induce specific disease cycles. The process leading to a vector's host shift and its epidemiological outcome is therefore embedded in the frameworks of sympatric evolution vs. immigration of preadapted populations. In this study, we analyse whether a host shift of the stolbur phytoplasma vector, Hyalesthes obsoletus from field bindweed to stinging nettle in its northern distribution range evolved sympatrically or by immigration. The exploitation of stinging nettle has led to outbreaks of the grapevine disease bois noir caused by a stinging nettle‐specific phytoplasma strain. Microsatellite data from populations from northern and ancestral ranges provide strong evidence for sympatric host‐race evolution in the northern range: Host‐plant associated populations were significantly differentiated among syntopic sites (0.054 < FHT < 0.098) and constant over 5 years. While gene flow was asymmetric from the old into the predicted new host race, which had significantly reduced genetic diversity, the genetic identity between syntopic host‐race populations in the northern range was higher than between these populations and syntopic populations in ancestral ranges, where there was no evidence for genetic host races. Although immigration was detected in the northern field bindweed population, it cannot explain host‐race diversification but suggests the introduction of a stinging nettle‐specific phytoplasma strain by plant‐unspecific vectors. The evolution of host races in the northern range has led to specific vector‐based bois noir disease cycles.
Spatial scale affects landscape genetic analysis of a wetland grasshopper
Authors: Keller Daniela , Holderegger Rolf , van Strien Maarten J. 
Published: March 2013

AbstractMost landscape genetic studies assess the impact of landscape elements on species' dispersal and gene flow. Many of these studies perform their analysis on all possible population pairs in a study area and do not explicitly consider the effects of spatial scale and population network topology on their results. Here, we examined the effects of spatial scale and population network topology on the outcome of a landscape genetic analysis. Additionally, we tested whether the relevant spatial scale of landscape genetic analysis could be defined by population network topology or by isolation‐by‐distance (IBD) patterns. A data set of the wetland grasshopperStethophyma grossum, collected in a fragmented agricultural landscape, was used to analyse population network topology,IBDpatterns and dispersal habitats, using least‐cost transect analysis. Landscape genetic analyses neglecting spatial scale and population network topology resulted in models with low fits, with which a most likely dispersal habitat could not be identified. In contrast, analyses considering spatial scale and population network topology resulted in high model fits by restricting landscape genetic analysis to smaller scales (0–3 km) and neighbouring populations, as represented by aGabriel graph. These models also successfully identified a likely dispersal habitat ofS. grossum. The above results suggest that spatial scale and potentially population network topology should be more explicitly considered in future landscape genetic analyses.