bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purification and partial characterisation of hatching factors for the potato cyst nematode Globodera rostochiensis from potato root leachate
Authors: Devine Ken , Jones Peter 
Published: July 2002

AbstractHatching factors with activity towards Globodera rostochiensis were isolated from the root leachates produced by 1500 potato plants grown in gravel. In total, nine hatching factors, each with molecular weight 530.5 Da, were isolated. The main differences in the daughter ions produced from different hatching factors after fragmentation by mass spectrometry occurred between 245 and 500 Da. The main hatching factor, HF E, was found in extremely low concentrations in the leachate (less than 2.9 × 10­5% of recovered organic material) and was active in vitro at less than 2.1 × 10­8 M. Separation of the different hatching factors during purification was found to decrease the percentage hatch achieved in vitro at optimum concentration. Aus Wurzelablaufwasser von 1500 in grobem Sand gehaltenen Kartoffelpflanzen wurden gegenüber Globodera rostochiensis wirksame Schlüpffaktoren isoliert. Insgesamt wurden neun Schlüpffaktoren isoliert, jeder mit einem Molekulargewicht von 530.5 Da. Die grössten Unterschiede bei den Tochterionen, die bei einer Zerlegung durch Massenspektrometrie erzielt wurden, traten zwischen 245 und 500 Da auf. Der Hauptschlüpffaktor, HF E, wurde in extrem niedrigen Konzentrationen (weniger als 2.9 × 10­5% des gewonnenen organischen Materials) im Ablaufwasser gefunden. Er war in vitro noch bei weniger als 2.1 × 10­8 M wirksam. Eine Trennung der verschiedenen Schlüpffaktoren im Laufe der Reinigung verminderte den prozentualen Schlupf in vitro bei optimaler Konzentration.
Description of Bursaphelenchus conicaudatus n. sp. (Nematoda: Aphelenchoididae), isolated from the yellow-spotted longicorn beetle, Psacothea hilaris (Coleoptera: Cerambycidae) and fig trees, Ficus carica
Authors: Kanzaki Natsumi , Tsuda Kaku , Futai Kazuyoshi 
Published: July 2002

Abstract
Bursaphelenchus conicaudatus n. sp. is described and figured. Specimens were
collected from a 3-week-old culture on Botrytis cinerea. The new species is
characterised by the roundish trapezoid bursa of males and tapered and
mucronate tail of females. The new species is closely related to B.
xylophilus, B. mucronatus and B. fraudulentus in the shapes of spicule and
vulval flap, but was easily distinguished from these three species by the
shapes of the bursa and female tail. Bursaphelenchus conicaudatus n. sp. est
décrit et illustré. Des spécimens ont été collectés à partir d’une culture
de 3 semaines sur Botrytis cinerea. La nouvelle espèce est caractérisée par
une bourse trapézoïdate et arrondie chez le mâle et par l’extrémité mucronée
de la queue chez la femelle. La nouvelle espèce est très proche de B.
xylophilus, B. mucronatus et B. fraudulentus par la forme des spicules et du
volet vulvaire mais elle a été facilement différenciée de ces trois espèces
par la forme de la bourse du mâle et de la queue de la femelle.
Five new species of Rhigonema Cobb, 1898 (Rhigonematida: Rhigonematidae) from the Philippines and Vietnam with observations on the morphology of the Indo-Malayan forms and nematophagy by R. voratum sp. n.
Authors: Hunt David 
Published: July 2002

AbstractFive new species of Rhigonema are described and illustrated from the gut of diplopods collected from the Philippines and Vietnam. R. flabellifer sp. n., R. golovatchi sp. n., R. rigonanae sp. n., R. spiridonovi sp. n. and R. voratum sp. n. are differentiated from one another and the remaining species in the genus by a combination of characters including form of the cephalic extremity, development and extent of the pilose region, female genital tract type, male and female tail shape, spicule form and number and disposition of the copulatory papillae. Two species, both from polydesmid diplopods, bear an unusual pedunculate, flap-like projection on each sector of the triangular oral aperture and have only thirteen copulatory papillae. Such projections have only previously been described from R. lanceacauda, a species from the West Indies that also shares a reduced and similarly arranged complement of thirteen male papillae. The morphology, bionomics and distribution of Rhigonema are discussed and evidence presented for the first time that nematophagy is practised by at least one species in the genus. Rhigonema leonilae (Sanchez-Velazquez, 1979) comb. n. is proposed for Ruizia leonilae Sanchez-Velazquez, 1979. Cinq nouvelles especes de Rhigonema Cobb, 1898 (Rhigonematida: Rhigonematidae) des Philippines et du Vietnam et observations sur la morphologie des formes indo-malaises et la nematophagie chez R. voratum sp. n. - Cinq nouvelles especes de Rhigonema provenant des intestins de diplopodes collectes aux Philippines et au Vietnam sont decrites et illustrees. R. flabellifer sp. n., R. golovatchi sp. n., R. rigonanae sp. n., R. spiridonovi sp. n. et R. voratum sp. n. sont differencies les uns des autres et des autres especes du genre par une combinaison de caracteres incluant la forme de l'extremite cephalique, le developpement et l'etendue de la pilosite, le type de tractus genital femelle, la forme de la queue du male et de la femelle, la forme des spicules et le nombre et la repartition des papilles copulatrices. Deux especes, provenant toutes deux de Diplopodes Polydesmides, portent une projection inhabituelle, pedonculee, en forme de languette, sur chacun des secteurs de l'ouverture buccale triangulaire et comptent seulement treize papilles copulatrices. De telles projections n'ont ete precedemment decrites que chez R.lanceacauda, une espece originaire des Antilles, qui exhibe egalement un cortege reduit de treize papilles chez le male avec la meme repartition. La morphologie, le comportement et la repartition des Rhigonema sont discutes et la preuve est apportee pour la premiere fois que la nematophagie est pratiquee par au moins une espece de ce genre. Rhigonema leonilae (Sanchez-Velazquez, 1979) comb. n. est propose pour Ruizia leonilae Sanchez-Velazquez, 1979.
Discussion of “Extracting K-Ar ages from shales: a theoretical test”
Authors: Ylagan R. F. , Pevear D. R. , Vrolijk P. J . 
Published: July 2002

AbstractIllite age analysis (Pevear, 1992) and similar mixing methods demonstrate nearlinear behaviour when corrected for smectite content in illite-smectite and substitutions for K+ in illite layers. Srodon (1999) found that highly curved mixing lines result from theoretical calculations and challenged the validity of IAA. However, our theoretical calculations indicate near-linear mixing lines result when corrected for the amount of smectite and known K+ substitutions (Na+ or NH4+). We conclude that perfect linearity is unattainable because IAA attempts to unmix a system linearly, but the system is based on an exponential function (K-Ar equation). In practice, we believe IAA can successfully determine the age range of illite diagenesis, even though perfect linearity is not attainable because: (1) significant deviations from linearity occur only if >500 Ma separate the diagenetic and detrital ages; (2) IAA analytical uncertainties result in diagenetic and detrital ages that span a range of ages.
Structural collapse of Al13- intercalated montmorillonite by Na- salicylate solutions
Authors: Molis E. , Thomas F. , Faisandier K. , Bihannic I. 
Published: July 2002

AbstractThe chemical, textural and structural transformations of Al13-intercalated montmorillonite, resulting from the depolymerization of the interlamellar Al13 polycations by Na salicylate solutions, were studied. Nitrogen gas adsorption shows a dramatic decrease in specific surface area from 493 to 39 m2g–1, due to the loss of microporosity. Modelling of small-angle X-ray scattering (SAXS) curves shows that the final product contains two phases: a Na-exchanged swelling phase accounting for 40% of the clay, and a fixed interlayer distance (20.8 Å ) phase accounting for 60% of the clay. The Al remaining in the clay galleries (45% of the initial Al) is composed of 13% Al13– and 87% hexacoordinated Al, probably Al(OH)03 and oligomeric Al-salicylate complexes, as shown by 27Al NMR. The instability of Al13-intercalated montmorillonite towards organic ligands is related to the hydration shell of the tridecamer, revealed by SAXS. These results focus on the necessity to take into account the presence of organic ligands in natural media when using materials such as Al13-intercalated clays.
Cationic ordering in oxide glasses: the example of transition elements
Authors: Galoisy L. , Cormier L. , Rossano S. , Ramos A. , Calas G. , Gaskell P. , Le Grand M. 
Published: July 2002

AbstractStructural data have been obtained on the cation surroundings in multi-component silicate and borosilicate glasses using chemically selective spectroscopic and scattering methods, such as extended X-ray absorption and neutron scattering with isotope substitution (NSIS). Transition elements such as Ni or Ti may occur in unusual 5-coordinated sites which coexist with other coordination numbers, depending on glass composition. Distribution of cationic sites in the glassy structure is responsible for unusual spectroscopic properties, as shown by Fe2+ Mössbauer spectroscopy. The environment of cations such as Zn, Zr or Mo, has been determined by EXAFS and discussed using the bond valence theory, which predicts the way to charge compensate the oxygen neighbours and which indicates the linkage of cationic sites with the silicate framework. Cation-cation correlations are given by NSIS up to ∼8 Á, indicating an extensive Medium Range Ordering (MRO) with corner- and edge-linked cationic polyhedra, for Ti and Ni-bearing glasses, respectively. This heterogeneous cationic distribution in glasses is consistent with the presence of two-dimensional domains in which cation mixing may occur, as shown in a Ca-Ni metasilicate glass. Three-dimensional domains have also been found by Ni-K edge EXAFS in the case of low alkali borate glasses, with a local structure which mimics some aspects of crystalline NiO. The presence of ordered cationic domains, clearly illustrated by Reverse Monte Carlo simulations helps to rationalize the physical properties of multi-component silicate glasses.
New data on welshite, e.g. Ca2Mg3.8Mn0.62+Fe0.12+Sb1.55+O2[Si2.8Be1.7Fe0.653+Al0.7As0.17O18], an aenigmatite-group mineral
Authors: Grew E. S. , Hålenius U. , Kritikos M. , Shearer C. K. 
Published: July 2002

AbstractElectron and ion microprobe data on two samples of welshite from the type locality of Långban, Sweden, gave analytical totals of 99.38–99.57 wt.% and BeO contents of 4.82–5.11 wt.%, corresponding to 1.692–1.773 Be/20 O. Mössbauer and optical spectra of one of these samples gave [iv]Fe3+/ΣFe = 0.91, [vi]Fe2+/ΣFe = 0.09, and no evidence of Mn3+. The resulting formula for this sample is Ca2Mg3.8Mn0.62+Fe0.12+Sb1.55+O2[Si2.8Be1.7Fe0.653+Al0.7As0.17O18, and that for the second sample, Ca2Mg3.8Mn0.12+Fe0.12+Fe0.83+Sb1.25+O2[Si2.8Be1.8Fe0.653+Al0.5As0.25O18], is related by the substitution involving tetrahedral and octahedral sites: 0.59[vi,iv](Fe,Al)3+ ≈ 0.42[vi](Mg,Mn,Fe)2+ + 0.21([vi]Sb,[iv]As)5+, i.e. 3[vi,iv]M3+ = 2[vi]M2+ + [vi,iv]M5+. Welshite is distinctive among aenigmatite-group minerals in the high proportion of Fe3+ in tetrahedral coordination and is unique in its Be content, substantially exceeding 1Be per formula unit. Given the cation distributions in other minerals related to aenigmatite, we think it is reasonable to assume that at least one tetrahedral site is >50% occupied by Be and that one octahedral site is >50% occupied by Sb, so that welshite should be retained as a distinct species with its own name in the aenigmatite group.
Evidence for hibbingite–kempite solid solution
Authors: Saini-Eidukat Bernhardt , Rudashevsky Nikolai S. , Polozov Alexander G. 
Published: July 2002

AbstractNew occurrences of hibbingite, γ-Fe2(OH)3Cl, have been found associated with platinum-group minerals in the Noril'sk Complex, and with the Korshunovskoye iron ores of the southern Siberian platform. The Norils'k grains, which are up to 0. 6 mm in diameter, are associated with the platinumgroup minerals froodite, cabriite, urvantsevite and with native silver in massive pentlandite–cubanite– chalcopyrite ore. The Korshunovskoye iron ore sample in which hibbingite was found is composed of fine-grained magnetite ore associated with halite. Hibbingite, hematite and silver grains are found in cavities in halite; the reddish-brown hibbingite grains usually occur as encrustations in the cavities. The size of hibbingite and hematite grains is up to 100 µm.Hibbingite from the Noril'sk Complex contains a significant kempite (Mn2(OH)3Cl) component; in some cases it contains over 50 mol. % Mn. These data suggest that at least a partial solid solution series exists between hibbingite and kempite. All known occurrences of hibbingite represent paragenetically late mineral assemblages. In the case of the Korshunovskoye deposits, the occurrences are associated with highly concentrated hydrothermal brines derived from the Lower Paleozoic saline sediments of the Siberian Platform cover.
Igneous layering in a dacite: on the origin and significance of Layer Cake Mountain, Kelowna, B.C., Canada
Authors: Greenough J. D. , Owen J. V. 
Published: July 2002

AbstractA Tertiary, dacitic volcanic land-form in Kelowna, British Columbia, shows layering that has not been recognized elsewhere. Layering is expressed as thin (0.5 m) layers separated by thick (4.5 m) layers exposed along a weathered fault scarp. The major elements show that both thick and thin layers are dacitic and geochemically very similar. Trace element modelling indicates that thin layers formed from thick layers via crystal fractionation involving removal of plagioclase, biotite and magnetite in the proportions 75:20:5, and with only 12% fractionation. The thin layers represent segregation veins generated during crystallization of the dacite. They formed when the crystal mush at the bottom of the upper crust successively, thermally contracted, fractured and foundered, siphoning evolved interstitial liquid from the mush into the horizontal crack. Cooling of the segregation veins led to further fracturing. Later, fluids following these fractures altered the thin layers and precipitated secondary carbonate minerals. The altered thin layers weather preferentially, thus visually accentuating the small primary chemical differences between thick and thin layers. The scale of layering, mode of formation and differentiation mechanisms appear different from those in felsic magma chambers and it is unclear how common this phenomenon is. However, similar layering is more easily identified and commonly developed in mafic lava flows.
Combined effect of all-trans retinoic acid and arsenic trioxide in acute promyelocytic leukemia cells in vitro and in vivo
Authors: Jing Yongkui , Wang Long , Xia Lijuan , Chen Guo-qiang , Chen Zhu , Miller Wilson H. , Waxman Samuel 
Published: July 2002

AbstractAll-trans retinoic acid (tRA) and arsenic trioxide (As2O3) induce non–cross-resistant complete clinical remission in patients with acute promyelocytic leukemia with t(15;17) translocation and target PML-RARα, the leukemogenic protein, by different pathways suggesting a possible therapeutic synergism. To evaluate this possibility, this study examined the effect of As2O3 on tRA-induced differentiation and, conversely, the effect of tRA on As2O3-induced apoptosis. As2O3 at subapoptotic concentrations (0.5 μM) decreased tRA-induced differentiation in NB4 cells but synergized with atRA to induce differentiation in tRA-resistant NB4 subclones MR-2 and R4 cells as measured by nitroblue tetrazolium reduction and tRA-inducible genes (TTGII, RARβ, RIG-E). tRA cleaved PML-RARα into distinct fragments in NB4 but not in tRA-resistant MR-2 or R4 cells, whereas As2O3 completely degraded PML-RARα in all 3 cell lines. As2O3-induced apoptosis was decreased by tRA pretreatment of NB4 cells but not of R4 cells and was associated with a strong induction of Bfl-1/A1 expression, a Bcl-2 protein family member. Severe combined immunodeficient mice bearing NB4 cells showed an additive survival effect after sequential treatment, but a toxic effect was observed after simultaneous treatment with tRA and As2O3. These data suggest that combined As2O3 and tRA treatment may be more effective than single agents in tRA-resistant patients. Although in vitro data do not always translate to in vivo response, toxicity and potential drug antagonism may be diminished by decreasing the concentration of As2O3 when given at the same time with therapeutic levels of tRA.
Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind α5 chain-containing human laminin with high affinity
Authors: Parsons Stephen F. , Lee Gloria , Spring Frances A. , Willig Thiebaut-Noel , Peters Luanne L. , Gimm J. Aura , Tanner Michael J. A. , Mohandas Narla , Anstee David J. , Chasis Joel Anne 
Published: July 2002

AbstractLutheran blood group glycoproteins (Lu gps) are receptors for the extracellular matrix protein, laminin. Studies suggest that Lu gps may contribute to vaso-occlusion in sickle cell disease and it has recently been shown that sickle cells adhere to laminin isoforms containing the α5 chain (laminin 10/11). Laminin α5 is present in the subendothelium and is also a constituent of bone marrow sinusoids, suggesting a role for the Lu/laminin interaction in erythropoiesis. The objectives of the current study were to define more precisely the molecular interactions of the extracellular and intracellular regions of human Lu and to clone and characterize a mouse homologue. To this end, complementary DNA and genomic clones for the mouse homologue were sequenced and the mouse Lu gene mapped to a region on chromosome 7 with conserved synteny with human 19q13.2. Mouse and human Lu gps are highly conserved (72% identity) at the amino acid sequence level and both mouse and human Lu gps specifically bind laminin 10/11 with high affinity. Furthermore, the first 3, N-terminal, immunoglobulin superfamily domains of human Lu are critical for this interaction. The results indicated that the cytoplasmic domain of BRIC 221-labeled human Lu gp is linked with the spectrin-based skeleton, affording the speculation that this interaction may be critical for signal transduction. These results further support a role for Lu gps in sickle cell disease and indicate the utility of mouse models to explore the function of Lu gp-laminin 10/11 interaction in normal erythropoiesis and in sickle cell disease.
Differential requirement for the transcription factor PU.1 in the generation of natural killer cells versus B and T cells
Authors: Colucci Francesco , Samson Sandrine I. , DeKoter Rodney P. , Lantz Olivier , Singh Harinder , Di Santo James P. 
Published: July 2002

AbstractPU.1 is a member of the Ets family of transcription factors required for the development of various lymphoid and myeloid cell lineages, but its role in natural killer (NK) cell development is not known. The study shows that PU.1 is expressed in NK cells and that, on cell transfer into alymphoid Rag2/γc−/−mice, hematopoietic progenitors of PU.1−/−fetal liver cells could generate functional NK cells but not B or T cells. Nevertheless, the numbers of bone marrow NK cell precursors and splenic mature NK cells were reduced compared to controls. Moreover,PU.1−/− NK cells displayed reduced expression of the receptors for stem cell factor and interleukin (IL)-7, suggesting a nonredundant role for PU.1 in regulating the expression of these cytokine receptor genes during NK cell development.PU.1−/− NK cells also showed defective expression of inhibitory and activating members of the Ly49 family and failed to proliferate in response to IL-2 and IL-12. Thus, despite the less stringent requirement for PU.1 in NK cell development compared to B and T cells, PU.1 regulates NK cell differentiation and homeostasis.
Quantitation of minimal disease levels in chronic lymphocytic leukemia using a sensitive flow cytometric assay improves the prediction of outcome and can be used to optimize therapy
Authors: Rawstron Andy C. , Kennedy Ben , Evans Paul A. S. , Davies Faith E. , Richards Stephen J. , Haynes Andy P. , Russell Nigel H. , Hale Geoff , Morgan Gareth J. , Jack Andrew S. , Hillmen Peter 
Published: July 2002

Abstract
Previous studies have suggested that the level of residual disease at the end of therapy predicts outcome in chronic lymphocytic leukemia (CLL). However, available methods for detecting CLL cells are either insensitive or not routinely applicable. A flow cytometric assay was developed that can differentiate CLL cells from normal B cells on the basis of their CD19/CD5/CD20/CD79b expression. The assay is rapid and can detect one CLL cell in 104 to 105leukocytes in all patients. We have compared this assay to conventional assessment in 104 patients treated with CAMPATH-1H and/or autologous transplant. During CAMPATH-1H therapy, circulating CLL cells were rapidly depleted in responding patients, but remained detectable in nonresponders. Patients with more than 0.01 × 109/L circulating CLL cells always had significant (&gt; 5%) marrow disease, and blood monitoring could be used to time marrow assessments. In 25 out of 104 patients achieving complete remission by National Cancer Institute (NCI) criteria, the detection of residual bone marrow disease at more than 0.05% of leukocytes in 6 out of 25 patients predicted significantly poorer event-free (P = .0001) and overall survival (P = .007). CLL cells are detectable at a median of 15.8 months (range, 5.5-41.8) posttreatment in 9 out of 18 evaluable patients with less than 0.05% CLL cells at end of treatment. All patients with detectable disease have progressively increasing disease levels on follow-up. The use of sensitive techniques, such as the flow assay described here, allow accurate quantitation of disease levels and provide an accurate method for guiding therapy and predicting outcome. These results suggest that the eradication of detectable disease may lead to improved survival and should be tested in future studies.
Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow
Authors: Turner Nancy A. , Moake Joel L. , McIntire Larry V. 
Published: July 2002

Abstract
Using heparinized whole blood and flow conditions, it was shown that adenosine 5′-diphosphate (ADP) receptors P2Y12 and P2Y1 are both important in direct shear-induced platelet aggregation and platelet aggregation subsequent to initial adhesion onto von Willebrand factor (vWf)–collagen. In the viscometer, whole blood was subjected to shear rates of 750, 1500, and 3000 s−1 for 30 seconds at room temperature. The extent of aggregation was determined by flow cytometry. The P2Y12antagonist AR-C69 931MX (ARMX) reduced shear-induced aggregation at these rates by 56%, 54%, and 16%, respectively, compared to control samples. Adenosine 3′,5′-diphosphate (A3P5P; P2Y1antagonist) inhibited shear-induced aggregation by 40%, 30% and 29%, respectively, compared to control samples. Blockade of both ADP receptors at 3000 s−1 with ARMX plus A3P5P further reduced the platelet aggregation by 41% compared to the addition of ARMX alone (57% compared to control samples). Using a parallel-plate flow chamber, whole blood was perfused over bovine collagen type 1 at a wall shear rate of 3000 s−1 for 60 seconds. Platelet deposition was quantified with epifluorescence video microscopy and digital image processing. Blockade of P2Y12 alone or blockade of P2Y1 alone did not reduce thrombus formation on vWf–collagen. In contrast, blockade of both P2Y12 and P2Y1 reduced platelet deposition by 72%. These results indicate that combinations of antagonists of the ADP receptors P2Y12 and P2Y1 are effective inhibitors of direct shear-induced platelet aggregation and of platelet aggregation subsequent to initial adhesion under flow conditions. Inhibitors of these pathways are potentially useful as antiarterial thrombotic agents.