bluuebunny/crossref_metadata_2025_split huggingface dataset:
From waste to soil: Can we create functioning manufactured soils by recycling rock processing waste?
Authors: Bucka Franziska B. , Guigue Julien , Reifschneider Lena , Pihlap Evelin , Garcia‐Franco Noelia , Kühnel Anna , Kögel‐Knabner Ingrid , Vidal Alix 
Published: July 2024

AbstractRock mining industries do not only exploit and transform extensive areas of land, but also produce vast amounts of rock waste material that lacks an adequate utilization. Some of these rock wastes have the potential to provide nutrients to plants and can therefore have positive impacts on soil properties. Consequently, we tested their potential for valorization as components of manufactured soils for use in urban areas. We conducted a 10‐week incubation experiment of soil mesocosms with sunflowers (Helianthus annuus L.) to evaluate the performance of manufactured soils with respect to plant growth and soil properties. We used three common rock materials (augite‐porphyry, greywacke‐hornfels, basalt), ground to powder and mixed into natural soils of either clayey or sandy texture. In order to test the performance under challenging environmental conditions, we applied a drought treatment in addition to a regular watering treatment. All manufactured soils were able to maintain plant growth, although the yield of aboveground biomass was significantly lower compared to the original soils. However, the effects of the water regime and the original soils on the overall plant growth were stronger than the effect of the rock powders, indicating that the manufactured soils were not hampering plant development more than challenging environmental conditions. The preparation of the manufactured soils altered the grain size distribution of the originally sandy and clayey soils. Since the rock powders contributed mainly to the silt‐sized particles, their addition to soils may improve the physical properties of the soil, especially the plant's available water content. We used wet‐sieving to isolate aggregate size fractions and thus analyse the formation of soil aggregates. The manufactured soils had a higher mass contribution of microaggregate‐sized particles, although this was mostly attributed to the presence of silt‐sized rock powder particles instead of aggregate formation. The total organic carbon (OC) content of the original soils was diluted in the manufactured soils, as the rock powders did not contain OC. However, the manufactured soils may have the potential for future OC storage due to the abundance of OC‐free mineral surfaces, which can retain organic matter as well as capture CO2 through enhanced weathering of primary minerals. Based on this work, the tested rock materials have the potential to be utilized as components in manufactured soils in the urban context that provide soil‐like functions, particularly in terms of sustaining plant growth. For improved results, additional measures to initiate a rapid development of soil structure are highly recommended, for example, by adding an easily decomposable source of organic matter.
Combining field measurements and process‐based modelling to analyse soil tillage and crop residues management impacts on crop production and carbon balance in temperate areas
Authors: Delandmeter Mathieu , Colinet Gilles , Pierreux Jérôme , Bindelle Jérôme , Dumont Benjamin 
Published: July 2024

AbstractCrop residues management is an important issue in the context of climate change. They might be kept on the field and restituted to the soil to enhance its fertility or exported for other uses such as the production of energy through biomethanization. Furthermore, the choices regarding tillage operations impact the potential to incorporate residues, which in turn affects soil physical (e.g. structure, water retention), biological (e.g. organic matter, microorganisms) and chemical (e.g. nutrient release through mineralization) fertility. We combined measurements from a 14‐year field experiment in the Hesbaye loamy region of Belgium and its simulation with the STICS soil‐crop model to investigate the impacts of soil tillage and crop residues management on crop production, soil characteristics and carbon balance. Four treatments were compared, where all combinations of the incorporation versus exportation of crop residues and conventional versus reduced tillage were tested. The comparison of field observations with model simulations proved that the STICS model is adequate to explore the impacts of such contrasted management. The combined analysis of field data and soil‐crop model outputs showed that crop production was positively influenced by conventional tillage but unresponsive to crop residues fate. Reduced tillage led to a clear stratification in observed SOC content in the topsoil (0–30 cm), but also to an increase in simulated SOC stocks (0–26 cm). This SOC gain led to greater water retention under reduced tillage. Moreover, in both tillage treatments, incorporating residues increased soil organic carbon despite the associated augmentation in soil heterotrophic respiration. Finally, the importance of environmental conditions in carbon balance suggests that crop modelling might be very useful to explore the impacts of soil tillage and crop residues management in specific agro‐pedoclimatic contexts, especially when facing climate change.
Perspectives on biovigilance and transmission of infection of donor origin in deceased organ donation—The evolving scenario in the United Kingdom
Authors: Ushiro‐Lumb Ines , Geoghegan Christie , McGowan Olive , Baker Richard 
Published: July 2024

AbstractBackgroundA biovigilance program that has oversight of the entire organ and tissue donation and transplantation pathways underpins the quality and safety system in the United Kingdom.MethodA synopsis of the microbiological characterization of potential deceased organ donors and the processes for notification and investigation of possible donor‐derived infections are described. A summary of the outcome of investigations performed over a 10‐year period and a subset data frame of 5 years showing the proportion of infection incidents in relation to other incident types are also presented.ConclusionA single, centralized system overseeing the entire donation and transplantation pathway has become an essential part of transplantation practice across the United Kingdom. Revision of processes and management options, awareness of clinical conditions, and review of guidance are some examples of benefits gained over time. Transmission figures provided reflect the UK setting; these should be interpreted in context, as donor and recipient epidemiology differs across regions and nations. Despite a well‐established system in place, under reporting of cases continues to occur, with ongoing efforts to reassure professionals and patients of the true benefits of biovigilance in driving improvements in practice and patient outcomes.
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Elevated serum gamma‐glutamyltransferase activity and immunohistochemistry in two dogs with renal carcinoma
Authors: Stowe Devorah M. , Held Elizabeth P. , Cross Emily A. , Meritet Danielle , Hess Paul R. , Ferris Kelli , Mochizuki Hiroyuki 
Published: July 2024

AbstractDuring a 3‐year time period, a 15‐year‐old male castrated Terrier mix (dog 1) and a 6‐year‐old female spayed Labrador Retriever (dog 2) presented to the North Carolina State Veterinary Hospital with similar blood work abnormalities and no significant physical examination findings. A CBC, chemistry panel, and urinalysis performed on both dogs were relatively unremarkable, other than a marked increase in serum gamma‐glutamyltransferase (GGT) activity. Through imaging, both patients were diagnosed with a renal mass, and histopathology of both masses revealed a carcinoma. Immunohistochemical staining of the renal mass in both dog 1 and dog 2 were intensely positive for GGT. Dog 1 had the affected kidney removed, which normalized the GGT value. Dog 2 was euthanized, and metastasis to the lung was noted upon postmortem examination. There have been limited case studies documenting an elevation in serum GGT in dogs diagnosed with renal carcinoma. While renal carcinoma is uncommon in dogs, it is an important differential to keep in mind when there is a marked increase in serum GGT without accompanying increases in other measured liver enzymes. In addition, serum GGT can serve as a helpful biomarker for disease resolution and recurrence, as surgical removal of the renal mass (dog 1) led to the resolution of the elevated serum GGT. To our knowledge, this is the first report demonstrating IHC staining for GGT in a canine renal carcinoma.
Residual Strain and Joint Pressurization Maintain Collagen Tension for On-Joint Lumbar Facet Capsular Ligaments
Authors: Gacek Elizabeth , Ellingson Arin M. , Barocas Victor H. 
Published: July 2024

Abstract
Modeling the lumbar facet capsular ligament's (FCL) mechanical behavior under various physiological motions has often been a challenge due to limited knowledge about the on-joint in situ ligament state arising from attachment to the bone or other internal loads. Building on prior work, this study presents an enhanced computational model of the lumbar facet capsular ligament by incorporating residual strain and joint pressurization strain, factors neglected in prior models. Further, the model can predict strain and stress distribution across the ligament under various spinal motions, highlighting the influence of the ligament's attachment to the bone, internal synovial fluid pressurization, and distribution of collagen fiber alignment on the overall mechanical response of the ligament. Joint space inflation was found to influence the total observed stress and strain fields, both at rest and during motion. A significant portion of the ligament was found to be in tension, even in the absence of external load. Additionally, the model's ability to account for residual strain offers a more realistic portrayal of the collagen fibers and elastin matrix's role in ligament mechanics. We conclude that (1) computational models of the lumbar facet capsular ligament should not assume that the ligament is unloaded when the joint is in its neutral position, and (2) the ligament is nearly always in tension, which may be important in terms of its long-term growth and remodeling.
Host species shapes genotype, antimicrobial resistance, and virulence profiles of enterotoxigenic
Escherichia coli
(ETEC) from livestock in the United States
Authors: Fu Yezhi , Nawrocki Erin M. , M’ikanatha Nkuchia M. , Dudley Edward G. 
Published: July 2024

ABSTRACT

Enterotoxigenic
Escherichia coli
(ETEC) are significant pathogen in both cattle and pigs, causing diarrhea in these animals and leading to economic losses in the livestock industry. Understanding the dissimilarity in genotype, antimicrobial resistance (AMR), and virulence between bovine and swine ETEC is crucial for development of targeted preventive and therapeutic approaches for livestock. However, a comprehensive study on this area remains lacking. Here, we performed whole-genome sequencing-based analyses of bovine (
n
= 554) and swine (
n
= 623) ETEC collected in the United States over a 53-year period. We identified distinct ETEC genotypes (
fimH
type, O antigen, H antigen, sequence type) in cattle and pigs. Furthermore, specific AMR and virulence profiles were associated with bovine and swine ETEC. Compared to swine ETEC, bovine ETEC were less diverse in genotypes and had a significantly (
P
< 0.001) lower number of AMR genes per isolate but higher co-occurrence of Shiga toxin and enterotoxin genes. Our results provide an overview of the key genomic differences between bovine and swine ETEC in the United States, which might be attributed to host adaptation and antibiotic usage practice. Ongoing surveillance and research are essential to monitor the genetic diversity and AMR patterns of ETEC in different host species.

IMPORTANCE

Enterotoxigenic
Escherichia coli
(ETEC)-associated diarrhea represent one of the most economically important diseases in the livestock industry. By analyzing over a thousand livestock-derived ETEC samples in the United States, our study unveiled a clear distinction in ETEC’s genetic traits (i.e., genotypes, antimicrobial resistance [AMR], and virulence profiles) that might be tied to the different use of antibiotics in cattle and pigs, and the bacteria’s adaptation to their specific animal hosts. This understanding is crucial for tailoring preventive and therapeutic strategies. It also highlights the significance of ongoing surveillance and research into the evolution of bacterial pathogens like ETEC in livestock by using advanced techniques such as whole-genome sequencing.
Association of oral fungal profiles with health status and bacterial composition in elderly adults receiving community support and home care service
Authors: Asakawa Mikari , Kageyama Shinya , Said Heba Shehta , Ma Jiale , Suma Shino , Furuta Michiko , Takeshita Toru 
Published: July 2024

ABSTRACT

Fungi compose a minority but a common component of normal oral microbiota and contribute to oral and systemic health by interacting with bacterial inhabitants. This study investigated the relationship of oral fungal profiles to health status and bacterial profiles of 159 elderly adults receiving community support and home care services. Fungal and bacterial densities and compositions were determined based on the fungal ribosomal internal transcribed spacer region and bacterial 16S rRNA gene amplicon analyses, respectively. The total fungal density of 87 individuals exceeded 5,000 copies, and their microbiota was characterized by significantly less dense bacterial populations and lower relative abundances of oral health-associated taxa, such as
Neisseria perflava
and
Porphyromonas pasteri
, compared with those with less than 5,000 copies of fungi. These individuals were significantly older, had fewer teeth, had lower physical function, and comprised more denture users and individuals with cognitive decline. Fungal compositions were classified into three profiles (
Candida albicans
-dominant, non-
albicans Candida
-dominant, and non-
Candida
-dominant), and individuals with a non-
albicans Candida
-dominant profile exhibited significantly lower physical and cognitive function than those with the
Candida albicans
-dominant profile. These results demonstrate that a high-density fungal population co-occurs with poor oral and systemic health status of the host and dysbiosis of the bacterial community, and particularly, the overgrowth of non-
albicans Candida
species may be implicated in worsening systemic conditions.

IMPORTANCE

The interaction between fungal and bacterial components involved in the virulence of oral microbiota has received attention. This study demonstrates that an increase in fungal components is associated with a dysbiotic bacterial community and poor health status in elderly adults. Among individuals with a high-density fungal population, particularly, those with a non-
albicans Candida
-dominant profile had lower physical and cognitive functions than those with a
C. albicans
-dominant profile. These findings indicate that the evaluation of fungal components, in addition to the bacterial components, is important to understand the involvement of oral microbiota in oral and systemic diseases in elderly adults.
Complete genome sequence of an Indian outbreak strain of chikungunya virus
Authors: Laha Eshna , Jena Deepak , Biswas Viplov K. , Singh Sharad , Raghav Sunil K. , Pattnaik Asit K. , Chattopadhyay Soma 
Published: July 2024

ABSTRACT

Here, we report the complete genome sequence of an Indian strain of chikungunya virus isolated from an infected patient from Hyderabad, Andhra Pradesh, India, during a massive outbreak in 2005–2006. The genome length spans 11,811 nucleotides and has a poly(A) tail of 29 residues at the 3′ end.
Complete genome sequence of
Bacillus paramobilis
sp. strain IMGN7 from soil
Authors: Jung Hoseong , Choi Sejin , Kim Yeongjun , Han Jeong A. , Lee Ho-Seok , Kim Eun Yu 
Published: July 2024

ABSTRACT

Here, we report the complete genome sequence of
Bacillus paramobilis
sp. strain IMGN7, which was isolated from soil in South Korea. Its complete genome size is 5.28 Mbp. This genome will provide various insights for further studies about their function as biocontrol agents, such as bioremediation and antibiosis.
High Precision Ultrasonic Testing Method for Density of Engineering Plastics
Authors: Li Chenggang , Wang Lun , Sun Lihong , Chu Zhaojie , Liu Wei , Tao Jiagui 
Published: July 2024

Abstract
The density of engineering plastics is a key parameter for ensuring their safety and reliability. In order to achieve rapid and high-precision on-site detection, a method based on the acoustic pressure reflection coefficient is proposed. First, finite element simulation analysis was conducted to obtain the acoustic field distribution during ultrasound propagation under water immersion conditions. The correlation between interface echo intensity and material density was determined. Optimal detection parameters were designed to reduce measurement errors caused by beam overlap and diffusion attenuation. A water immersion ultrasonic experimental system was constructed, and the measurement accuracy of the method was tested using chlorinated polyvinyl chloride pipes. The results show that, compared to the measurement results of the Archimedean drainage method, the maximum error of ultrasonic measurements does not exceed 1.7%, and the overall variance is less than 1.2%. The measurement accuracy of this method is compared with the regression results of different machine learning models. It is demonstrated that, compared to regression methods based on variable correlation, this method retains the advantages of high efficiency and low cost in ultrasonic density measurement, while achieving higher measurement accuracy. Additionally, it does not require a dataset for training support, making it promising and valuable for practical applications.
Integrated care for children and young people with special health and care needs: a systematic review
Authors: Ghotane Swapnil , Hirve Raeena , Forman Julia , Tan Daniel , Achercouk Zak , Wolfe Ingrid 
Published: July 2024

ContextThere is a dearth of high-quality evidence on integrated, coordinated and cost-effective care for children with special health and care needs (CSHCN).ObjectiveTo assess the effectiveness of integrated/coordinated care models for CSHCN.Data sourcesEmbase, Ovid Medline(R), HMIC Health Management Information Consortium, Maternity & Infant Care Database (MIDIRS), PsycARTICLES, PsycINFO, Social Policy and Practice, Cochrane Central Register of Controlled Trials (CENTRAL), Global Health and PubMed.Study selectionInclusion criteria comprised (1) randomised trials, including cluster randomised trials; (2) an integrated/coordinated care intervention; (3) for children and young people under 25 with special healthcare needs including medical complexity; (4) assessing child-centred outcomes, health-related quality of life among parents and carers, and health or social care use, processes of care and satisfaction with care.Data extractionData were extracted and assessed by two researchers, and descriptive data were synthesised according to outcome and intervention.Results14 randomised controlled studies were included. Seven out of the 14 studies had a dedicated key worker/care coordinator as a vital part of the integrated/coordinated care intervention; however, the certainty of evidence for all outcomes was either ‘low’ or ‘very low’.LimitationsIncluded studies were mostly from high-income countries. Variable study outcomes and quality of evidence precluded meta-analysis.ConclusionsLimited evidence favours integrated care for CSHCN using a dedicated key worker/care coordinator; however, heterogeneity in study outcomes and definitions of CSHCN limit the strength and utility of evidence obtained. Recommendations are made for improving integrated care practice, research and evaluation which are important for evidence-based health services for CSHCN.PROSPERO registration numberCRD42020209320.
Preclinical study and parallel phase II trial evaluating antisense STAT3 oligonucleotide and checkpoint blockade for advanced pancreatic, non-small cell lung cancer and mismatch repair-deficient colorectal cancer
Authors: Tang Chad , Hartley Genevieve P , Couillault Coline , Yuan Ying , Lin Heather , Nicholas Courtney , Srinivasamani Anupallavi , Dai James , Dumbrava Ecaterina E Ileana , Fu Siqing , Karp Daniel D , Naing Aung , Piha-Paul Sarina A , Rodon Ahnert Jordi , Pant Shubham , Subbiah Vivek , Yap Timonthy A , Tsimberidou Apostolia M , Guerrero Paola , Dhebat Sarah , Proia Theresa , Curran Michael A , Hong David S 
Published: July 2024

Objective
To evaluate signal transducer and activator of transcription 3 (STAT3) inhibition we conducted a co-clinical trial testing danvatirsen, a STAT3 antisense oligonucleotide (ASO) and checkpoint inhibition in conjunction with preclinical experiments.

Methods and analysis
Orthotopically implanted pancreatic cancer (pancreatic adenocarcinoma (PDAC)) was treated with STAT3 ASO with immune checkpoint inhibition. Tumour infiltrating immune cell populations were characterised via flow cytometry. In vitro experiments evaluated STAT3 inhibition in pancreatic stellate cells (PSCs) and myeloid-derived suppressor cells (MDSCs).
A phase II trial employing a Simon II stage design tested the clinical efficacy of danvatirsen and durvalumab in non-small cell lung cancer (NSCLC), PDAC and mismatch repair-deficient colorectal cancer (MRD CRC). The primary objective was 4-month disease control rate (DCR).

Results
In vivo studies identified improvement in survival of PDAC implanted mice treated with STAT3 ASO and checkpoint inhibition. Within tumour-infiltrating lymphocytes there was expansion of CD4 and PD-1+ CD8 populations with STAT3 ASO.
Thirty-seven patients (29 PDAC, 7 NSCLC and 1 MRD CRC) from a single institution started treatment on trial between April 2017 and March 2020. No objective responses were observed. Four of six (66.7%, 95% CI 22.3% to 95.7%) NSCLC and 4 of 23 (17.4%, 95% CI 5% to 38.8%) PDAC patients exhibited 4-month DCR. Follow-up in vitro studies revealed an anti-inflammatory and pro-tumour effect of STAT3 ASO mediated by PSCs and MDSCs distinct from ablation of STAT3.

Conclusion
Although durvalumab and danvatirsen met the primary endpoint, no objective responses were observed. A rationale for the lack of objective responses is danvatirsen-induced myeloid immune suppression.

Trial registration number

NCT02983578
.