bluuebunny/crossref_metadata_2025_split huggingface dataset:
Secondary biliary cirrhosis in the rat is prevented by decreasing NF-κ B nuclear translocation and TGF-β expression using allopurinol, an inhibitor of xanthine oxidase
Authors: Aldaba-Muruato Liseth R. , Moreno Mario G. , Hernández-Mercado Elisa , Shibayama Mineko , Muriel Pablo 
Published: November 2012

Allopurinol is an inhibitor of xanthine oxidase (XO), and XO is an enzyme that generates great amounts of reactive oxygen species. The aim of this work was to evaluate the efficacy of allopurinol to prevent experimental cirrhosis. Fibrosis and cirrhosis were induced by common bile duct ligation (BDL) for 4 weeks in rats. Animals were divided into 4 groups: sham-operated rats (SHAM); BDL group; BDL plus allopurinol (100 mg·kg–1, p.o.), and SHAM plus allopurinol treatment. Alanine aminotransferase, γ-glutamyl transpeptidase, and alkaline phosphatase were increased in BDL rats but were preserved normal by allopurinol. XO activity was prevented by allopurinol; however, lipophilic and hydrophilic oxidative stress was not prevented by the drug. Allopurinol partially suppresses nuclear factor-κB (NF-κB) nuclear translocation and transforming growth factor-β (TGF-β) expression, and increased the active form of matrix metalloproteinase-13 (MMP-13). Moreover, collagen production induced by BDL was partially but significantly reduced by allopurinol. These findings suggest that allopurinol possesses a hepatoprotective effect probably by modulating proteins such as NF-κB, TGF-β, and MMP-13, helping to protect against liver damage induced by chronic cholestasis and a mechanism independent of oxidative stress.
Anticancer activity and mechanism of juglone on human cervical carcinoma HeLa cells
Authors: Zhang Wei , Liu Anheng , Li Yan , Zhao Xingyu , Lv Shijie , Zhu Wenhe , Jin Ying 
Published: November 2012

Induction of apoptosis in tumor cells has become the major focus of anti-tumor therapeutics development. Juglone, a major chemical constituent of Juglans mandshurica Maxim, possesses several bioactivities, including anti-tumor. In the present study, HeLa cells were incubated with juglone at various concentrations. The proliferation inhibition of juglone on HeLa cells was tested by the MTT assay. Occurrence of apoptosis was detected by Hoechst 33258 staining, flow cytometry, and transmission electron microscopy. The expression of apoptotic-related proteins was examined by Western blot. The results showed that juglone inhibits the growth of HeLa cells in dose–dependent manner. Topical morphological changes of apoptotic body formation after juglone treatment were observed. The percentages of early apoptosis of Annexin V-FITC were 5.23%, 7.95%, 10.69%, and 20.92% with the concentrations of juglone (12.5, 25, 50, and 100 µmol/L), respectively. After cells were treated with juglone at the different dose for 24 h, the expression of Bcl-2 was significantly down-regulated and the expression of Bax was significantly up-regulated compared with the control. These events paralleled with activation of caspase-9, -8, -3, and PARP cleavage. The results suggest that juglone may be effective for the treatment of HeLa cells.
In vitro and in vivo evaluation of the effect of elevated factor VIII on the thrombogenic process
Authors: Golder Mia , Mewburn Jeffrey , Lillicrap David 
Published: November 2012

SummaryFactor VIII (FVIII), a procoagulant cofactor, plays a crucial role in the intrinsic coagulation cascade. A causal association between elevated FVIII levels and venous thrombosis incidence has been established; no such association has been confirmed with arterial thrombosis. The independent role of elevated FVIII levels in arteriolar thrombosis was evaluated in a mouse model to determine the thrombogenic potential of elevated levels of FVIII. The in vitro thrombogenic effect of elevated FVIII levels was examined using thrombin-antithrombin (TAT) complex generation and thromboelastography (TEG) assays. The thrombogenic potential of acute and extended elevation of circulating FVIII levels was assessed using ferric chloride induced injury of the cremaster arterioles. The rate of TAT complex formation, and the final concentration of TAT complexes, significantly increased as FVIII levels were elevated from 100% to 400% FVIII activity. TEG analysis of fibrin and clot formation showed that as FVIII levels were elevated, the time to initial fibrin formation decreased and the rate of fibrin formation increased. The acute elevation of circulating FVIII to 400% FVIII activity resulted in significantly decreased times to vessel occlusion. Prolonged elevation of FVIII activity did not significantly affect time to vessel occlusion. In conclusion, acute elevations in FVIII levels result in a nonlinear thrombogenic effect, with non-significant increases in thrombogenic risk within the physiological range (FVIII levels up to 200%). Prolonged elevation of plasma FVIII did not further increase the thrombogenic potential of elevated FVIII levels.
Simulations of the Eastern North Pacific Intraseasonal Variability in CMIP5 GCMs
Authors: Jiang Xianan , Maloney Eric D. , Li Jui-Lin F. , Waliser Duane E. 
Published: November 2012

Abstract
As a key component of tropical atmospheric variability, intraseasonal variability (ISV) over the eastern North Pacific Ocean (ENP) exerts pronounced influences on regional weather and climate. Since general circulation models (GCMs) are essential tools for prediction and projection of future climate, current model deficiencies in representing this important variability leave us greatly disadvantaged in studies and prediction of climate change. In this study, the authors have assessed model fidelity in representing ENP ISV by analyzing 16 GCMs participating in phase 5 of the Coupled Model Intercomparison Project (CMIP5). Among the 16 CMIP5 GCMs examined in this study, only seven GCMs capture the spatial pattern of the leading ENP ISV mode relatively well, although even these GCMs exhibit biases in simulating ISV amplitude. Analyses indicate that model fidelity in representing ENP ISV is closely associated with the ability to simulate a realistic summer mean state. The presence of westerly or weak mean easterly winds over the ENP warm pool region could be conducive to more realistic simulations of the ISV. One hypothesis to explain this relationship is that a realistic mean state could produce the correct sign of surface flux anomalies relative to the ISV convection, which helps to destabilize local intraseasonal disturbances. The projected changes in characteristics of ENP ISV under the representative concentration pathway 8.5 (RCP8.5) projection scenario are also explored based on simulations from three CMIP5 GCMs. Results suggest that, in a future climate, the amplitude of ISV could be enhanced over the southern part of the ENP while reduced over the northern ENP off the coast of Mexico/Central America and the Caribbean.
A Second-Moment Closure Model of Langmuir Turbulence
Authors: Harcourt Ramsey R. 
Published: November 2012

Abstract
The Reynolds stress equation is modified to include the Craik–Leibovich vortex force, arising from the interaction of the phase-averaged surface wave Stokes drift with upper-ocean turbulence. An algebraic second-moment closure of the Reynolds stress equation yields an algebraic Reynolds stress model (ARSM) that requires a component of the vertical momentum flux to be directed down the gradient of the Stokes drift, in addition to the conventional component down the gradient of the ensemble-averaged Eulerian velocity. For vertical and horizontal component fluctuations, the momentum flux must be closed using the form , where the coefficient is generally distinct from the eddy viscosity or eddy diffusivity . Rational expressions for the stability functions , , and are derived for use in second-moment closure models where the turbulent velocity and length scales are dynamically modeled by prognostic equations for and . The resulting second-moment closure (SMC) includes the significant effects of the vortex force in the stability functions, in addition to source terms contributing to the and equations. Additional changes are made to the way in which is limited by proximity to boundaries or by stratification. The new SMC model is tuned to, and compared with, a suite of steady-state large-eddy simulation (LES) solutions representing a wide range of oceanic wind and wave forcing conditions. Comparisons with LES show the modified SMC captures important processes of Langmuir turbulence, but not without notable defects that may limit model generality.
Hydrographic Preconditioning for Seasonal Sea Ice Anomalies in the Labrador Sea
Authors: Fenty Ian , Heimbach Patrick 
Published: November 2012

Abstract
This study investigates the hydrographic processes involved in setting the maximum wintertime sea ice (SI) extent in the Labrador Sea and Baffin Bay. The analysis is based on an ocean and sea ice state estimate covering the summer-to-summer 1996/97 annual cycle. The estimate is a synthesis of in situ and satellite hydrographic and ice data with a regional coupled ⅓° ocean–sea ice model. SI advective processes are first demonstrated to be required to reproduce the observed ice extent. With advection, the marginal ice zone (MIZ) location stabilizes where ice melt balances ice mass convergence, a quasi-equilibrium condition achieved via the convergence of warm subtropical-origin subsurface waters into the mixed layer seaward of the MIZ.
An analysis of ocean surface buoyancy fluxes reveals a critical role of low-salinity upper ocean (100 m) anomalies for the advancement of SI seaward of the Arctic Water–Irminger Water Thermohaline Front. Anomalous low-salinity waters slow the rate of buoyancy loss–driven mixed layer deepening, shielding an advancing SI pack from the warm subsurface waters, and are conducive to a positive surface meltwater stabilization enhancement (MESEM) feedback driven by SI meltwater release. The low-salinity upper-ocean hydrographic conditions in which the MESEM efficiently operates are termed sea ice–preconditioned waters (SIPW).
The SI extent seaward of the Thermohaline Front is shown to closely correspond to the distribution of SIPW. The analysis of two additional state estimates (1992/93, 2003/04) suggests that interannual hydrographic variability provides a first-order explanation for SI maximum extent anomalies in the region.
Student Pharmacists’ Perceptions of Barriers to Medication Adherence Counseling
Authors: Mangan Michelle N. , Powers Mary F. , Lengel Aaron J. 
Published: November 2012

Purpose: To identify the barriers perceived by student pharmacists to counseling on medication adherence in a supermarket community pharmacy chain. Methods: Online surveys were made available to 65 student pharmacists. Subjects completed the survey anonymously and indicated their opinions and perceptions on topics related to medication adherence through the use of a 5-point Likert-type scale. Results: Of the surveyed student pharmacists, 26 completed the survey (40%). All participants (100%) agreed that they have knowledge on the importance of medication adherence. Student pharmacists stated that they did not have the time to cover adherence in a counseling session (72.7%) nor did they have time to perform follow-up phone calls with the patient (54.5%). The majority of student pharmacists (81.8%) were comfortable bringing up the topic of adherence with the patient but thought that they did not have access to resources on adherence to provide to the patient (59%). Conclusions: Surveyed students indicated they were constrained for time to counsel patients on adherence and to perform continued follow-up with patients afterward. Factors cited as barriers to counseling patients on medication adherence include time, lack of specific training in adherence management, lack of resources to provide to the patient, and patient disinterest in the subject.
Modified Brain Stem Auditory Evoked Potentials in Patients With Intracranial Mass Lesions
Authors: Stone James L. , Fino John , Patel Ketan , Calderon-Arnulphi Mateo , Suss Nichole , Hughes John R. 
Published: November 2012

The authors report their experience utilizing a recently described rapid rate, binaural click and 1000-Hz tone burst modification of the brain stem auditory evoked potentials (BAEP), modified (MBP), in 27 symptomatic patients with non–brain stem compressive space-taking cerebral lesions (22), hydrocephalus (4), and pseudotumor cerebri (1). Many presented with clinical signs suggestive of increased intracranial pressure (ICP) and focal neurological deficits. The cerebral lesions, mostly large tumors with edema, had very substantial radiological signs of mass effect. Fourteen patients were also studied following surgical decompression. A number of significant changes in the wave V and Vn latency/intensity and less so amplitude/intensity function was found in the 27 patients, compared to normal volunteers, as well as those studied pre- and postoperatively. Similar MBP changes had been noted in normal volunteers placed in a dependent head position. Possible mechanisms to explain these findings are discussed. The MBP methodology shows promise and further development could make neuro-intensive care unit monitoring practical.
B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequences
Authors: Gomes de Oliveira Sarah , Cassia de Moura Rita , Martins Cesar 
Published: November 2012

Abstract

Background
To contribute to the knowledge of coleopteran cytogenetics, especially with respect to the genomic content of B chromosomes, we analyzed the composition and organization of repetitive DNA sequences in the Coprophanaeus cyanescens karyotype. We used conventional staining and the application of fluorescence in situ hybridization (FISH) mapping using as probes C

0

t-1 DNA fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable element (TE).

Results
The conventional analysis detected 3 individuals (among 50 analyzed) carrying one small metacentric and mitotically unstable B chromosome. The FISH analysis revealed a pericentromeric block of C

0

t-1 DNA in the B chromosome but no 18S or 5S rDNA clusters in this extra element. Using the LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in eight autosomal bivalents and in the B chromosome, and a pericentromeric block extending to the short arm in one autosomal pair. No positive hybridization signal was observed for the LOA-like element in the sex chromosomes.

Conclusions
The results indicate that the origin of the B chromosome is associated with the autosomal elements, as demonstrated by the hybridization with C

0

t-1 DNA and the LOA-like TE. The present study is the first report on the cytogenetic mapping of a TE in coleopteran chromosomes. These TEs could have been involved in the origin and evolution of the B chromosome in C. cyanescens.
Rapid transcriptome characterization and parsing of sequences in a non-model host-pathogen interaction; pea-Sclerotinia sclerotiorum
Authors: Zhuang Xiaofeng , McPhee Kevin E , Coram Tristan E , Peever Tobin L , Chilvers Martin I 
Published: November 2012

Abstract

Background
White mold, caused by Sclerotinia sclerotiorum, is one of the most important diseases of pea (Pisum sativum L.), however, little is known about the genetics and biochemistry of this interaction. Identification of genes underlying resistance in the host or pathogenicity and virulence factors in the pathogen will increase our knowledge of the pea-S. sclerotiorum interaction and facilitate the introgression of new resistance genes into commercial pea varieties. Although the S. sclerotiorum genome sequence is available, no pea genome is available, due in part to its large genome size (~3500 Mb) and extensive repeated motifs. Here we present an EST data set specific to the interaction between S. sclerotiorum and pea, and a method to distinguish pathogen and host sequences without a species-specific reference genome.

Results
10,158 contigs were obtained by de novo assembly of 128,720 high-quality reads generated by 454 pyrosequencing of the pea-S. sclerotiorum interactome. A method based on the tBLASTx program was modified to distinguish pea and S. sclerotiorum ESTs. To test this strategy, a mixture of known ESTs (18,490 pea and 17,198 S. sclerotiorum ESTs) from public databases were pooled and parsed; the tBLASTx method successfully separated 90.1% of the artificial EST mix with 99.9% accuracy. The tBLASTx method successfully parsed 89.4% of the 454-derived EST contigs, as validated by PCR, into pea (6,299 contigs) and S. sclerotiorum (2,780 contigs) categories. Two thousand eight hundred and forty pea ESTs and 996 S. sclerotiorum ESTs were predicted to be expressed specifically during the pea-S. sclerotiorum interaction as determined by homology search against 81,449 pea ESTs (from flowers, leaves, cotyledons, epi- and hypocotyl, and etiolated and light treated etiolated seedlings) and 57,751 S. sclerotiorum ESTs (from mycelia at neutral pH, developing apothecia and developing sclerotia). Among those ESTs specifically expressed, 277 (9.8%) pea ESTs were predicted to be involved in plant defense and response to biotic or abiotic stress, and 93 (9.3%) S. sclerotiorum ESTs were predicted to be involved in pathogenicity/virulence. Additionally, 142 S. sclerotiorum ESTs were identified as secretory/signal peptides of which only 21 were previously reported.

Conclusions
We present and characterize an EST resource specific to the pea-S. sclerotiorum interaction. Additionally, the tBLASTx method used to parse S. sclerotiorum and pea ESTs was demonstrated to be a reliable and accurate method to distinguish ESTs without a reference genome.
Elevation of soluble major histocompatibility complex class I related chain A protein in malignant and infectious diseases in Chinese patients
Authors: Jiang Xiaoxin , Huang Ju-Fang , Huo Zhi , Zhang Qiuqui , Jiang Yan , Wu Xiaoping , Li Yanwen , Jiang Guanmin , Zeng Leping , Yan Xiao-Xin , Yu Ping , Cao Renxian 
Published: November 2012

Abstract

Background
Elevation of soluble major histocompatibility complex class I chain-related gene A (sMICA) products in serum has been linked to tissue/organ transplantation, autoimmune diseases and some malignant disorders. Cells infected by microbiological pathogens may release sMICA, whereas less is known whether and to what extent serum sMICA levels may change in infectious diseases.

Methods
The present study determined serum sMICA levels by enzyme-linked immunosorbent assay (ELISA) in a southern China population, including patients (n = 1041) suffering from several types of malignant and infectious diseases and healthy controls (n = 141).

Results
Relative to controls, serum sMICA elevation was significant in patients of hepatic cancer, and was approaching statistical significance in patients with lung, gastric and nasopharyngeal cancers. sMICA elevation was also associated with some bacterial (Enterobacteriaceae, Mycobacterium tuberculosis, non-fermenting Gram-negative bacteria and Gram-positive cocci), viral (hepatitis B and C) and the Microspironema pallidum infections.

Conclusion
Serum sMICA levels may be informative for the diagnosis of some malignant and infectious diseases. The results also indicate that microbiological infections should be considered as a potential confounding clinical condition causing serum sMICA elevation while using this test to evaluate the status of other disorders, such as cancers, host-graft response and autoimmune diseases.
Age- and gender-related prevalence of multimorbidity in primary care: the swiss fire project
Authors: Rizza Alessandro , Kaplan Vladimir , Senn Oliver , Rosemann Thomas , Bhend Heinz , Tandjung Ryan 
Published: November 2012

AbstractBackgroundGeneral practitioners often care for patients with several concurrent chronic medical conditions (multimorbidity). Recent data suggest that multimorbidity might be observed more often than isolated diseases in primary care. We explored the age- and gender-related prevalence of multimorbidity and compared these estimates to the prevalence estimates of other common specific diseases found in Swiss primary care.MethodsWe analyzed data from the Swiss FIRE (Family Medicine ICPC Research using Electronic Medical Record) project database, representing a total of 509,656 primary care encounters in 98,152 adult patients between January 1, 2009 and July 31, 2011. For each encounter, medical problems were encoded using the second version of the International Classification of primary Care (ICPC-2). We defined chronic health conditions using 147 pre-specified ICPC-2 codes and defined multimorbidity as 1) two or more chronic health conditions from different ICPC-2 rubrics, 2) two or more chronic health conditions from different ICPC-2 chapters, and 3) two or more medical specialties involved in patient care. We compared the prevalence estimates of multimorbidity defined by the three methodologies with the prevalence estimates of common diseases encountered in primary care.ResultsOverall, the prevalence estimates of multimorbidity were similar for the three different definitions (15% [95%CI 11-18%], 13% [95%CI 10-16%], and 14% [95%CI 11-17%], respectively), and were higher than the prevalence estimates of any specific chronic health condition (hypertension, uncomplicated 9% [95%CI 7-11%], back syndrome with and without radiating pain 6% [95%CI 5-7%], non-insulin dependent diabetes mellitus 3% [95%CI 3-4%]), and degenerative joint disease 3% [95%CI 2%-4%]). The prevalence estimates of multimorbidity rose more than 20-fold with age, from 2% (95%CI 1-2%) in those aged 20–29 years, to 38% (95%CI 31-44%) in those aged 80 or more years. The prevalence estimates of multimorbidity were similar for men and women (15% vs. 14%, p=0.288).ConclusionsIn primary care, prevalence estimates of multimorbidity are higher than those of isolated diseases. Among the elderly, more than one out of three patients suffer from multimorbidity. Management of multimorbidity is a principal concern in this vulnerable patient population.