bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural Flood Management Through Peatland Restoration: Catchment‐Scale Modeling of Past and Future Scenarios in Glossop, UK
Authors: Goudarzi Salim , Milledge David , Holden Joseph , Evans Martin , Allott Tim , Johnston Adam , Shuttleworth Emma , Kay Martin , Brown David , Rees Joe , Edokpa Donald , Spencer Tom 
Published: August 2024

AbstractField‐scale experiments have shown the Natural Flood Management (NFM) potential of peatland restoration. The likelihoods of effectiveness are yet unknown at scales and storms large enough to impact human lives. Using GMD‐TOPMODEL, we upscale a rare Before‐After‐Control‐Intervention empirical data set to a 25 km2 catchment with >600 properties at flood‐risk, and test storms of up to a 1,000‐year return period (RP). Under these scales/storms, we find that it is not necessary (nor feasible) to delay the outlet flow‐peak to meaningfully attenuate it. Enhancing catchment “kinematic” storage, for example, through restoration, can be sufficient to reduce flow magnitudes without detectable changes to peak‐flow timing. NFM benefit increases exponentially with restoration area size under smaller storms, but linearly under larger storms. At RP ≤ 100 years, longer‐lasting frontal‐type storms are more challenging to defend against via NFM, but at RP > 100 years shorter‐duration convectional‐type events become more challenging. In the order of 1,000–10 years storms: (a) revegetating the bare‐peat areas in 15% of the catchment is 31%–61% likely to reduce peak‐flows by >5%; (b) revegetating & damming the erosion gullies in ∼20% of the catchment is 42%–71% likely to reduce peak‐flows by >5%; (c) Growth of Sphagnum in the dammed gullies of ∼20% and ∼40% of the catchment increase the likelihoods of >5% peak reductions to 65%–86% and 90%–98%, respectively. The numerical evidence of significant NFM benefit due to Sphagnum re‐establishment is an important finding, because it shows that meaningful flood‐risk mitigation in headwater catchments under scales/storms relevant to communities at risk can be delivered alongside other ecosystem benefits of Sphagnum re‐establishment.
Micro/nanofabrication of heat management materials for energy-efficient building facades
Authors: Wang Guanya , Ryu Keunhyuk , Dong Zhaogang , Hu Yuwei , Ke Yujie , Dong ZhiLi , Long Yi 
Published: August 2024

AbstractAdvanced building facades, which include windows, walls, and roofs, hold great promise for reducing building energy consumption. In recent decades, the management of heat transfer via electromagnetic radiation between buildings and outdoor environments has emerged as a critical research field aimed at regulating solar irradiation and thermal emission properties. Rapid advancements have led to the widespread utilization of advanced micro/nanofabrication techniques. This review provides the first comprehensive summary of fabrication methods for heat management materials with potential applications in energy-efficient building facades, with a particular emphasis on recent developments in fabrication processing and material property design. These methods include coating, vapor deposition, nanolithography, printing, etching, and electrospinning. Furthermore, we present our perspectives regarding their advantages and disadvantages and our opinions on the opportunities and challenges in this field. This review is expected to expedite future research by providing information on the selection, design, improvement, and development of relevant fabrication techniques for advanced materials with energy-efficient heat management capabilities.
On-chip dielectrophoretic single-cell manipulation
Authors: Tian Zuyuan , Wang Xihua , Chen Jie 
Published: August 2024

AbstractBioanalysis at a single-cell level has yielded unparalleled insight into the heterogeneity of complex biological samples. Combined with Lab-on-a-Chip concepts, various simultaneous and high-frequency techniques and microfluidic platforms have led to the development of high-throughput platforms for single-cell analysis. Dielectrophoresis (DEP), an electrical approach based on the dielectric property of target cells, makes it possible to efficiently manipulate individual cells without labeling. This review focusses on the engineering designs of recent advanced microfluidic designs that utilize DEP techniques for multiple single-cell analyses. On-chip DEP is primarily effectuated by the induced dipole of dielectric particles, (i.e., cells) in a non-uniform electric field. In addition to simply capturing and releasing particles, DEP can also aid in more complex manipulations, such as rotation and moving along arbitrary predefined routes for numerous applications. Correspondingly, DEP electrodes can be designed with different patterns to achieve different geometric boundaries of the electric fields. Since many single-cell analyses require isolation and compartmentalization of individual cells, specific microstructures can also be incorporated into DEP devices. This article discusses common electrical and physical designs of single-cell DEP microfluidic devices as well as different categories of electrodes and microstructures. In addition, an up-to-date summary of achievements and challenges in current designs, together with prospects for future design direction, is provided.
KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1
Authors: Li Mingxuan , Fang Qian , Xiao Pingping , Yin Zhinang , Mei Guangbo , Wang Cheng , Xiang Ying , Zhao Xuejun , Qu Lihua , Xu Tian , Zhang Jiaxi , Liu Kejun , Li Xiaoqing , Dong Huifen , Xiao Ruijing , Zhou Rui 
Published: August 2024

AbstractAcute liver failure (ALF) is characterized by the rapidly progressive deterioration of hepatic function, which, without effective medical intervention, results in high mortality and morbidity. Here, using proteomic and transcriptomic analyses in murine ALF models, we found that the expression of multiple splicing factors was downregulated in ALF. Notably, we found that KH-type splicing regulatory protein (KHSRP) has a protective effect in ALF. Knockdown of KHSRP resulted in dramatic splicing defects, such as intron retention, and led to the exacerbation of liver injury in ALF. Moreover, we demonstrated that KHSRP directly interacts with splicing factor 3b subunit 1 (SF3B1) and enhances the binding of SF3B1 to the intronic branch sites, thereby promoting pre-mRNA splicing. Using splicing inhibitors, we found that Khsrp protects against ALF by regulating pre-mRNA splicing in vivo. Overall, our findings demonstrate that KHSRP is an important splicing activator and promotes the expression of genes associated with ALF progression by interacting with SF3B1; thus, KHSRP could be a possible target for therapeutic intervention in ALF.
NLRP4 renders pancreatic cancer resistant to olaparib through promotion of the DNA damage response and ROS-induced autophagy
Authors: Xiao Mingming , Yang Jing , Dong Mingwei , Mao Xiaoqi , Pan Haoqi , Lei Yalan , Tong Xuhui , Yu Xiaoning , Yu Xianjun , Shi Si 
Published: August 2024

AbstractOlaparib has been approved as a therapeutic option for metastatic pancreatic ductal adenocarcinoma patients with BRCA1/2 mutations. However, a significant majority of pancreatic cancer patients have inherent resistance or develop tolerance to olaparib. It is crucial to comprehend the molecular mechanism underlying olaparib resistance to facilitate the development of targeted therapies for pancreatic cancer. In this study, we conducted an analysis of the DepMap database to investigate gene expression variations associated with olaparib sensitivity. Our findings revealed that NLRP4 upregulation contributes to increased resistance to olaparib in pancreatic cancer cells, both in vitro and in vivo. RNA sequencing and Co-IP MS analysis revealed that NLRP4 is involved in the DNA damage response and autophagy pathway. Our findings confirmed that NLRP4 enhances the capacity for DNA repair and induces the production of significant levels of reactive oxygen species (ROS) and autophagy in response to treatment with olaparib. Specifically, NLRP4-generated mitochondrial ROS promote autophagy in pancreatic cancer cells upon exposure to olaparib. However, NLRP4-induced ROS do not affect DNA damage. The inhibition of mitochondrial ROS using MitoQ and autophagy using chloroquine (CQ) may render cells more susceptible to the effects of olaparib. Taken together, our findings highlight the significant roles played by NLRP4 in the processes of autophagy and DNA repair when pancreatic cancer cells are treated with olaparib, thereby suggesting the potential therapeutic utility of olaparib in pancreatic cancer patients with low NLRP4 expression.
BRG1 promotes progression of B-cell acute lymphoblastic leukemia by disrupting PPP2R1A transcription
Authors: Kang Qian , Ma Dan , Zhao Peng , Chai Xiao , Huang Yi , Gao Rui , Zhang Tianzhuo , Liu Ping , Deng Bo , Feng Cheng , Zhang Yan , Lu Yinghao , Li Yanju , Fang Qin , Wang Jishi 
Published: August 2024

AbstractDespite advancements in chemotherapy and the availability of novel therapies, the outcome of adult patients with B-cell acute lymphoblastic leukemia (B-ALL) remains unsatisfactory. Therefore, it is necessary to understand the molecular mechanisms underlying the progression of B-ALL. Brahma-related gene 1 (BRG1) is a poor prognostic factor for multiple cancers. Here, the expression of BRG1 was found to be higher in patients with B-ALL, irrespective of the molecular subtype, than in healthy individuals, and its overexpression was associated with a poor prognosis. Upregulation of BRG1 accelerated cell cycle progression into the S phase, resulting in increased cell proliferation, whereas its downregulation facilitated the apoptosis of B-ALL cells. Mechanistically, BRG1 occupies the transcriptional activation site of PPP2R1A, thereby inhibiting its expression and activating the PI3K/AKT signaling pathway to regulate the proto-oncogenes c-Myc and BCL-2. Consistently, silencing of BRG1 and administration of PFI-3 (a specific inhibitor targeting BRG1) significantly inhibited the progression of leukemia and effectively prolonged survival in cell-derived xenograft mouse models of B-ALL. Altogether, this study demonstrates that BRG1-induced overactivation of the PPP2R1A/PI3K/AKT signaling pathway plays an important role in promoting the progression of B-ALL. Therefore, targeting BRG1 represents a promising strategy for the treatment of B-ALL in adults.
TREM2-dependent activation of microglial cell protects photoreceptor cell during retinal degeneration via PPARγ and CD36
Authors: Zhou Wenchuan , He Jincan , Shen Guiyan , Liu Ya , Zhao Peiquan , Li Jing 
Published: August 2024

AbstractRetinal degeneration is a collection of devastating conditions with progressive loss of vision which often lead to blindness. Research on retinal microglial cells offers great therapeutic potential in deterring the progression of degeneration. This study explored the mechanisms underlying the TREM2-mediated protective function of activated microglial cells during retinal degeneration. N-methyl-N-nitrosourea (MNU)-induced retinal degeneration was established in C57BL/6 J (WT) and Trem2 knockout (Trem2−/−) mice. We discovered that MNU treatment led to the concurrent processes of photoreceptor apoptosis and microglia infiltration. A significant upregulation of disease-associated microglia signature genes was observed during photoreceptor degeneration. Following MNU treatment, Trem2−/− mice showed exacerbated photoreceptor cell death, decreased microglia migration and phagocytosis, reduced microglial PPARγ activation and CD36 expression. Pharmaceutical activation of PPARγ promoted microglial migration, ameliorated photoreceptor degeneration and restored CD36 expression in MNU-treated Trem2−/− mice. Inhibition of CD36 activity worsened photoreceptor degeneration in MNU-treated WT mice. Our findings suggested that the protective effect of microglia during retinal degeneration was dependent on Trem2 expression and carried out via the activation of PPARγ and the consequent upregulation of CD36 expression. Our study linked TREM2 signaling with PPARγ activation, and provided a potential therapeutic target for the management of retinal degeneration.
TRIP13 regulates progression of gastric cancer through stabilising the expression of DDX21
Authors: Zhang Guanghui , Yang Rui , Wang Baiyan , Yan Qiujin , zhao Peiyuan , Zhang Jiaming , Su Weiyu , Yang Lianhe , Cui Hongjuan 
Published: August 2024

AbstractGC (Gastric cancer) is one of the most common malignant tumours, with over 95% of gastric cancer patients being adenocarcinoma and most gastric cancer patients having no apparent symptoms in the early stages. Finding biomarkers for early screening of gastric cancer and exploring new targets for gastric cancer treatment are urgent problems to be solved in the treatment of gastric cancer, with significant clinical outcomes for the survival rate of gastric cancer patients. The AAA+ family ATPase thyroid hormone receptor-interacting protein 13 (TRIP13) has been reported to play an essential role in developing various tumours. However, the biological function and molecular mechanism of TRIP13 in gastric cancer remain unclear. This study confirms that TRIP13 is highly expressed in gastric cancer tissue samples and that TRIP13 participates in the proliferation, migration, invasion in vitro, and tumourigenesis and metastasis in vivo of gastric cancer cells. Mechanistically, this study confirms that TRIP13 directly interacts with DDX21 and stabilises its expression by restraining its ubiquitination degradation, thereby promoting gastric cancer progression. Additionally, histone deacetylase 1 (HDAC1) is an upstream factor of TRIP13, which could target the TRIP13 promoter region to promote the proliferation, migration, and invasion of gastric cancer cells. These results indicate that TRIP13 serve is a promising biomarker for the treating of gastric cancer patients, and the HDAC1-TRIP13/DDX21 axis might provide a solid theoretical basis for clinical treatment of gastric cancer patients.
Missense BICD2 variants in fetuses with congenital arthrogryposis and pterygia
Authors: Masuda Layla , Hasegawa Akihiro , Kamura Hiromi , Hasegawa Fuyuki , Yamamura Michihiro , Taniguchi Kosuke , Ito Yuki , Hata Kenichiro , Samura Osamu , Okamoto Aikou 
Published: August 2024

Abstract
Type 2 spinal muscular atrophy with lower extremity dominance (SMALED2) is caused by bicaudal D cargo adaptor 2 (BICD2) variants. However, the SMALED2 genotype and phenotype correlation have not been thoroughly characterized. We identified de novo heterozygous BICD2 missense variants in two fetuses with severe, prenatally diagnosed multiple arthrogryposis congenita. This report provides further insights into the genetics of this rare disease.
Observation of nonlinear thermoelectric effect in MoGe/Y3Fe5O12
Authors: Arisawa Hiroki , Fujimoto Yuto , Kikkawa Takashi , Saitoh Eiji 
Published: August 2024

AbstractThermoelectric effects refer to the voltage generation from temperature gradients in condensed matter. Although various power generators are made from them, all the known effects, such as Seebeck effect, require macroscopic temperature gradients; since the sign of the generated voltage is reversed by reversing the temperature gradient, the net voltage disappears when the temperature distribution fluctuates temporarily or spatially with a macroscopic temperature gradient of zero. It is impossible to utilize such temperature fluctuations in the conventional thermoelectric effects, a situation which limits their application. Here we report the observation of a second-order nonlinear thermoelectric effect; we develop a method to measure nonlinear thermoelectricity and observe that a superconducting MoGe film on Y3Fe5O12 generates a voltage proportional to the square of the applied temperature gradient. The nonlinear thermoelectric generation demonstrated here provides a way for making power generators that produce electric power from temperature fluctuations.
Structure prediction of alternative protein conformations
Authors: Bryant Patrick , Noé Frank 
Published: August 2024

AbstractProteins are dynamic molecules whose movements result in different conformations with different functions. Neural networks such as AlphaFold2 can predict the structure of single-chain proteins with conformations most likely to exist in the PDB. However, almost all protein structures with multiple conformations represented in the PDB have been used while training these models. Therefore, it is unclear whether alternative protein conformations can be genuinely predicted using these networks, or if they are simply reproduced from memory. Here, we train a structure prediction network, Cfold, on a conformational split of the PDB to generate alternative conformations. Cfold enables efficient exploration of the conformational landscape of monomeric protein structures. Over 50% of experimentally known nonredundant alternative protein conformations evaluated here are predicted with high accuracy (TM-score > 0.8).
Polarity transitions of narrow bipolar events in thundercloud tops reaching the lower stratosphere
Authors: Liu Feifan , Neubert Torsten , Chanrion Olivier , Lu Gaopeng , Wu Ting , Lyu Fanchao , Lyu Weitao , Köhn Christoph , Li Dongshuai , Zhu Baoyou , Lei Jiuhou 
Published: August 2024

AbstractBlue corona discharges are often generated in thunderclouds penetrating into the stratosphere and are the optical manifestation of narrow bipolar events (NBEs) observed in radio signals. While their production appears to depend on convection, the cause and nature of such discharges are not well known. Here we show the observations by a lightning detection array of unusual amounts of 982 NBEs during a tropical storm on the coastline of China. NBEs of negative polarity are predominantly observed at the cloud top reaching the stratosphere, and positive NBEs are primarily at lower altitudes. We find that the dominant polarity changes with the typical time of development of thunderstorm cells, suggesting that the polarity depends on the phase of the storm cells. Furthermore, we find that the lightning jump of negative NBEs is associated with above-anvil cirrus plumes of ice crystals and water vapor in the lower stratosphere. We propose that variations in updrafts induce changes in the altitude and charge concentrations of the cloud layers, which lead to the polarity transition. Our results have implications for studies of the chemical perturbations of greenhouse gas concentrations by corona discharges at the tropopause.
Proteomic changes in Alzheimer’s disease associated with progressive Aβ plaque and tau tangle pathologies
Authors: Pichet Binette Alexa , Gaiteri Chris , Wennström Malin , Kumar Atul , Hristovska Ines , Spotorno Nicola , Salvadó Gemma , Strandberg Olof , Mathys Hansruedi , Tsai Li-Huei , Palmqvist Sebastian , Mattsson-Carlgren Niklas , Janelidze Shorena , Stomrud Erik , Vogel Jacob W. , Hansson Oskar 
Published: August 2024

AbstractProteomics can shed light on the dynamic and multifaceted alterations in neurodegenerative disorders like Alzheimer’s disease (AD). Combining radioligands measuring β-amyloid (Aβ) plaques and tau tangles with cerebrospinal fluid proteomics, we uncover molecular events mirroring different stages of AD pathology in living humans. We found 127 differentially abundant proteins (DAPs) across the AD spectrum. The strongest Aβ-related proteins were mainly expressed in glial cells and included SMOC1 and ITGAM. A dozen proteins linked to ATP metabolism and preferentially expressed in neurons were independently associated with tau tangle load and tau accumulation. Only 20% of the DAPs were also altered in other neurodegenerative diseases, underscoring AD’s distinct proteome. Two co-expression modules related, respectively, to protein metabolism and microglial immune response encompassed most DAPs, with opposing, staggered trajectories along the AD continuum. We unveil protein signatures associated with Aβ and tau proteinopathy in vivo, offering insights into complex neural responses and potential biomarkers and therapeutics targeting different disease stages.
High-speed aerial grasping using a soft drone with onboard perception
Authors: Ubellacker Samuel , Ray Aaron , Bern James M. , Strader Jared , Carlone Luca 
Published: August 2024

AbstractContrary to the stunning feats observed in birds of prey, aerial manipulation and grasping with flying robots still lack versatility and agility. Conventional approaches using rigid manipulators require precise positioning and are subject to large reaction forces at grasp, which limit performance at high speeds. The few reported examples of high-speed aerial grasping rely on motion capture systems, or fail to generalize across environments and grasp targets. We describe the first example of a soft aerial manipulator equipped with a fully onboard perception pipeline, capable of robustly localizing and grasping visually and morphologically varied objects. The proposed system features a novel passively closed tendon-actuated soft gripper that enables fast closure at grasp, while compensating for position errors, complying to the target-object morphology, and dampening reaction forces. The system includes an onboard perception pipeline that combines a neural-network-based semantic keypoint detector, a state-of-the-art robust 3D object pose estimator, and a fixed-lag smoother to estimate the pose of known objects. The resulting pose estimate is passed to a minimum-snap trajectory planner, tracked by an adaptive controller that fully compensates for the added mass of the grasped object. Finally, a finite-element-based controller determines optimal gripper configurations for grasping. Experiments on three different targets confirm that our approach enables dynamic, high-speed, and versatile grasping, all of which are necessary capabilities for tasks such as rapid package delivery or emergency relief. We demonstrate fully onboard vision-based grasps of a variety of objects, in both indoor and outdoor environments, and up to speeds of 2.0 m/s—the fastest vision-based grasp reported in the literature. Finally, we take a major step in expanding the utility of our platform beyond stationary targets, by demonstrating motion-capture-based grasps of targets moving up to 0.3 m/s, with relative speeds up to 1.5 m/s.
Anti‐sloshing control: Flatness‐based trajectory planning and tracking control with an integrated extended state observer
Authors: Viet Khanh Nguyen , Duc Minh Do , Duc Thanh Cao , Nguyen Tung Lam 
Published: August 2024

AbstractThe phenomenon of sloshing causes a significantly negative impact on a wide range of industries. A time‐optimal flatness‐based trajectory planning and Lyapunov‐based model predictive control (LMPC) is proposed for trajectory tracking of a transmitting cylindrical container filled with liquid. Firstly, this research presents an equivalent discrete model based on a mass‐spring‐damper system. Subsequently, after the flatness of the adopted non‐linear model for 2D is established, time‐optimal trajectories are introduced. A control method called LMPC is shown to solve the problem of orbital tracking, which allows setting limits for state variables. In addition, to ensure system performance, a linear extended state observer (LESO) is integrated to cope with system uncertainties. Finally, the efficiency of the proposed approach for liquid sloshing suppression and tracking is illustrated by simulations.