bluuebunny/crossref_metadata_2025_split huggingface dataset:
Equine model for soft‐tissue regeneration
Authors: Bellas Evangelia , Rollins Amanda , Moreau Jodie E. , Lo Tim , Quinn Kyle P. , Fourligas Nicholas , Georgakoudi Irene , Leisk Gary G. , Mazan Melissa , Thane Kristen E. , Taeymans Olivier , Hoffman A. M. , Kaplan D. L. , Kirker‐Head C. A. 
Published: October 2014

AbstractSoft‐tissue regeneration methods currently yield suboptimal clinical outcomes due to loss of tissue volume and a lack of functional tissue regeneration. Grafted tissues and natural biomaterials often degrade or resorb too quickly, while most synthetic materials do not degrade. In previous research we demonstrated that soft‐tissue regeneration can be supported using silk porous biomaterials for at least 18 months in vivo in a rodent model. In the present study, we scaled the system to a survival study using a large animal model and demonstrated the feasibility of these biomaterials for soft‐tissue regeneration in adult horses. Both slow and rapidly degrading silk matrices were evaluated in subcutaneous pocket and intramuscular defect depots. We showed that we can effectively employ an equine model over 6 months to simultaneously evaluate many different implants, reducing the number of animals needed. Furthermore, we were able to tailor matrix degradation by varying the initial format of the implanted silk. Finally, we demonstrate ultrasound imaging of implants to be an effective means for tracking tissue regeneration and implant degradation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1217–1227, 2015.
In vitro and in vivo evaluation of doxycycline‐chondroitin sulfate/PCLmicrospheres for intraarticular treatment of osteoarthritis
Authors: Aydin Ozlem , Korkusuz Feza , Korkusuz Petek , Tezcaner Aysen , Bilgic Elif , Yaprakci Volkan , Keskin Dilek 
Published: October 2014

AbstractOsteoarthritis (OA) is a degenerative joint disease, which has no complete treatment with medication yet. Intraarticular hyaluronan (HA) injection can decrease pain and modify the natural course of OA. This study was designed to provide long term delivery of an MMP (matrix‐metalloproteinase) inhibitor agent‐doxycycline, together with matrix regenerative agent‐chondroitin sulfate for treating OA which progress with matrix degenerations. Doxycycline (D) and doxycycline‐chondroitin sulfate (D‐CS) loaded poly‐ɛ‐caprolactone (PCL) microspheres (MS) were prepared as intraarticular delivery systems. Bio‐effectiveness of developed microspheres was first evaluated with three‐dimensional in vitro model of OA where both MS showed significant reduction in MMP‐13 levels compared to untreated OA‐chondrocytes at 15 and 24 days. Significant decrease was observed in GAG release into the media for both D MS and D‐CS MS treated groups at 15 and 24 days. Second, the microspheres were injected to rabbit knee in hyaluronan (HA) to evaluate the effectiveness of the treatment. Radiographic scores of D MS and D‐CS MS groups improved after 8 weeks when compared to OA group. Mankin‐Pitzker histological scores similarly showed improvement with D MS and D‐CSMS groups when compared to OA group. Ex vivo hardness tests of cartilages demonstrated superior hardness values with both doses of D‐CSMS compared to OA group. D MS showed promising improvement of OA in histology results. Although, both MS groups had similar effects on cells in the in vitro model, D‐CSMS had a positive contribution on all in vivo treatment outcomes and showed potential as a new strategy for treatment when applied to OA knee joints. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1238–1248, 2015.
Three‐dimensional constructs using hyaluronan cell carrier as a tool for the study of cancer stem cells
Authors: Martínez‐Ramos Cristina , Lebourg Myriam 
Published: October 2014

AbstractBackground: Cancer research focuses increasingly on cancer stem cell study as those cells are thought to be the root of chemo and radioresistance of the most aggressive cancer types. Nevertheless, two‐dimensional (2D) cell culture and even three‐dimensional (3D) spheroid models, with their limited ability to reflect cell‐extracellular matrix interactions, are not ideal for the study of cancer stem cells (CSCs). In this study, we establish a 3D in vitro cancer model using a synthetic and natural scaffold with tunable features and show that U87 cells cultured in this system acquire a stem‐cell like phenotype. Methods: U87 astrocytoma cells were grown on polycaprolactone (PCL)−2D flat substrates (2D) and PCL‐3D scaffolds (3D) eventually containing hyaluronic acid (3D‐HA). Cell viability, growth patterns, morphology, and cell surface marker expression (CD44, RHAMM and CD133) were studied to assess the effect of 3D culture and presence of HA. Results: 3D scaffold, but most prominently presence of HA induced changes in cell morphology and marker expression; 3D‐HA cultures showed features of aggregates; moreover, markedly increased expression of Nestin, CD44, RHAMM, and CD133 in 3D‐HA scaffolds were found. Conclusions: the behavior of U87 in our 3D‐HA model is more similar to tumor growth in vivo and a stem‐like phenotype is promoted. Thus, the 3D‐HA scaffold could provide a useful model for CSCs study and anti‐cancer therapeutics research in vitro and may have preclinical application for the screening of drug candidates. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1249–1257, 2015.
Real‐time, noninvasive optical coherence tomography of cross‐sectional living cell‐sheets in vitro and in vivo
Authors: Kobayashi Mari , Haraguchi Yuji , Shimizu Tatsuya , Mizuuchi Kiminori , Iseki Hiroshi 
Published: October 2014

AbstractCell sheet technology has a history of application in regenerating various tissues, having successfully completed several clinical trials using autologous cell sheets. Tomographic analysis of living cell sheets is an important tool in the field of cell sheet‐based regenerative medicine and tissue engineering to analyze the inner structure of layered living cells. Optical coherence tomography (OCT) is commonly used in ophthalmology to noninvasively analyze cross‐sections of target tissues at high resolution. This study used OCT to conduct real‐time, noninvasive analysis of living cell sheet cross sections. OCT showed the internal structure of cell sheets in tomographic images synthesized with backscatter signals from inside the living cell sheet without invasion or damage. OCT observations were used to analyze the static and dynamic behaviors of living cell sheets in vitro and in vivo including (1) the harvesting process of a C2C12 mouse skeletal myoblast sheet from a temperature‐responsive culture surface; (2) cell‐sheet adhesion onto various surfaces including a culture surface, a synthetic rubber glove, and the dorsal subcutaneous tissue of rats; and (3) the real‐time propagation of beating rat cardiac cells within cardiac cell sheets. This study showed that OCT technology is a powerful tool in the field of cell sheet‐based regenerative medicine and tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1267–1273, 2015.
Effect of faldaprevir on raltegravir pharmacokinetics in healthy volunteers
Authors: Joseph David , Rose Peter , Strelkowa Natalja , Schultz Armin , Garcia Jeanette , Elgadi Mabrouk , Huang Fenglei 
Published: October 2014

AbstractFaldaprevir is a potent hepatitis C virus (HCV) NS3/4A protease inhibitor and an inhibitor of UDP‐glucuronosyltransferase‐1A1 (UGT1A1), which is involved in raltegravir clearance. Raltegravir, an HIV integrase inhibitor, may be used in combination with HCV treatment in HCV/HIV co‐infected patients. In this open‐label, 2‐period, fixed‐sequence study, 24 healthy volunteers (12 males) received faldaprevir 240 mg and raltegravir 400 mg in 2 treatment schedules (A and B) separated by a washout phase of ≥7 days: (A) twice‐daily raltegravir (Days 1–3), once‐daily raltegravir (Day 4); (B) twice‐daily raltegravir and twice‐daily faldaprevir (loading dose, Day 1), twice‐daily raltegravir and once‐daily faldaprevir (Days 2–5), once‐daily raltegravir and once‐daily faldaprevir (Day 6). Pharmacokinetics and safety were assessed over 132 hours post‐dosing. Compared with raltegravir alone, co‐administration with faldaprevir led to 2.7‐fold and 2.5‐fold increases in raltegravir geometric mean AUCτ,ss and Cmax,ss, respectively, and a similar increase in raltegravir glucuronide metabolite exposure. No serious adverse events (AEs) were reported and no subject discontinued due to AEs. Faldaprevir and raltegravir co‐administration was well tolerated and resulted in a moderate increase in raltegravir exposure.
Regular Graphs of Odd Degree Are Antimagic
Authors: Cranston Daniel W. , Liang Yu‐Chang , Zhu Xuding 
Published: October 2014

AbstractAn antimagic labeling of a graph G with m edges is a bijection from to such that for all vertices u and v, the sum of labels on edges incident to u differs from that for edges incident to v. Hartsfield and Ringel conjectured that every connected graph other than the single edge K2 has an antimagic labeling. We prove this conjecture for regular graphs of odd degree.
Chonopeltis australis (Crustacea) male reproductive system morphology; Sperm transfer and review of reproduction in Branchiura
Authors: Neethling Lourelle Alicia Martins , Avenant‐Oldewage Annemariè 
Published: October 2014

ABSTRACTThe morphology of the male reproductive system as well as sperm transfer in Branchiura has been described for Dolops ranarum and Argulus japonicus. In this study, the reproductive system and accessory structures are described for male Chonopeltis australis using histology, light microscopy, and scanning electron microscopy. For the first time, we describe sperm transfer by means of a spermatophore in this genus. The internal and external morphology and mechanism of sperm transfer is compared with other Branchiura, where it has been described. The morphology of the reproductive system of C. australis is similar to that of D. ranarum while the accessory structures and the spermatophore produced are similar to that of A. japonicus. A revision of the definition of Branchiura with respect to reproduction is provided. J. Morphol. 276:209–218, 2015. © 2014 Wiley Periodicals, Inc.
Impact assessment of mechanical harvest on fruit physiology and consequences on oil physicochemical and sensory quality from ‘Manzanilla de Sevilla’ and ‘Manzanilla Cacereña’ super‐high‐density hedgerows. A preliminary study
Authors: Morales‐Sillero Ana , García José M 
Published: October 2014

AbstractBACKGROUNDSuper‐intensive cultivation facilitates olive mechanized harvesting, allowing substantial savings in the production cost of virgin olive oil (VOO). However, the number of varieties adapted to this type of cultivation is small. This study explores the impact that harvesting with a grape straddle harvester of ‘Manzanilla de Sevilla’ and ‘Manzanilla Cacereña’ olives grown in super‐intensive cultivation has on the physiology of the fruit and the quality of the oil subsequently extracted.RESULTSFor both cultivars, fruits harvested mechanically showed higher respiration and ethylene production and lower firmness than fruits harvested by hand. Their oils exhibited lower phenol contents, lower oxidative stability and lower presence of positive sensory attributes. However, in these oils the values of parameters used to assess the level of quality ofVOOremained within the limits required for the best commercial category.CONCLUSIONMechanical harvesting of ‘Manzanilla de Sevilla’ and ‘Manzanilla Cacereña’ super‐high‐density hedgerows induced physiological alterations in the fruits and a reduction in the contents of natural antioxidants and flavour components in the oils, though it did not result in a loss of the ‘Extra’ level of quality. © 2014 Society of Chemical Industry
Effect of white wheat bread and white wheat bread added with bioactive compounds on hypercholesterolemic and steatotic mice fed a high‐fat diet
Authors: Pozzo Luisa , Pucci Laura , Buonamici Guglielmo , Giorgetti Lucia , Maltinti Maristella , Longo Vincenzo 
Published: October 2014

AbstractBACKGROUNDThe effects of white wheat bread and white wheat bread added with a bioactive compound mixture (Cyclanthera pedata, Glycine max, Monascus‐fermented red mold rice, Cynara scolymus and Medicago sativa) were examined on hypercholesterolemic and steatotic mice, divided into four groups: control diet (CTR), high‐fat diet (HFD), high‐fat diet with white wheat bread added with 1.5 g kg−1 of mixture (HFD+AB) and high‐fat diet with white wheat bread (HFD+B).RESULTSTotal serum cholesterol in the HFD+AB and HFD+B groups and hepatic triglycerides in the HFD+AB group decreased compared with the HFD group. Liver histology confirmed lower lipid drop accumulation in the HFD+AB group than in the HFD and HFD+B groups. HFD+AB caused a 7.0‐fold increase and a 3.5‐fold reduction in CYP7A1 and SREBP‐1c gene expression respectively compared with the HFD group. Moreover, the HFD+B group showed a 2.2‐, 8.4‐ and 1.5‐fold increase in HMG CoA reductase, CYP7A1 and LDLr gene expression respectively compared with the HFD group.CONCLUSIONBoth the white wheat bread and the added white wheat bread induced cholesterol reduction by increasing CYP7A1. Moreover, the added white wheat bread improved steatosis by decreasing SREBP‐1c gene expression. © 2014 Society of Chemical Industry
Carnosic acid attenuates obesity‐induced glucose intolerance and hepatic fat accumulation by modulating genes of lipid metabolism in C57BL/6J‐ob/ob mice
Authors: Park Mi‐Young , Sung Mi‐Kyung 
Published: October 2014

AbstractBACKGROUNDCarnosic acid (CA), a major bioactive component of rosemary (Rosmarinus officinalis) leaves, is known to possess antioxidant and anti‐adipogenic activities. In this study it was hypothesized that CA would ameliorate obesity‐induced glucose intolerence and hepatic fat accumulation, and possible mechanisms are suggested.RESULTSIt was observed that a 0.02% (w/w) CA diet effectively decreased body weight, liver weight and blood triglyceride (TG) and total cholesterol levels (P < 0.05) compared with the control diet. CA at 0.02% significantly improved glucose tolerance, and hepatic TG accumulation was reduced in a dose‐dependent manner. Hepatic lipogenic‐related gene (L‐FABP, SCD1 and FAS) expression decreased whereas lipolysis‐related gene (CPT1) expression increased in animals fed the 0.02% CA diet (P < 0.05). Long‐chain fatty acid content and the ratio of C18:1/C18:0 fatty acids were decreased in adipose tissue of animals fed the 0.02% CA diet (P < 0.05). Serum inflammatory mediators were also decreased significantly in animals fed the 0.02% CA diet compared with those of the obese control group (P < 0.05).CONCLUSIONThese results suggest that CA is an effective anti‐obesity agent that regulates fatty acid metabolism in C57BL/6J‐ob/ob mice. © 2014 Society of Chemical Industry
Can Benefits in Recreational Parks in Malaysia Be Transferred? A Choice Experiment (CE) Technique
Authors: Hasan‐Basri Bakti , Abd Karim Mohd Zaini 
Published: October 2014

AbstractThis paper analyses benefit transfer in the case of recreational parks using the choice experiment (CE) technique. The CE was employed because it allows different changes in recreational park attributes to be taken into account. The analyses were performed in terms of transferability of valuation function and willingness to pay (WTP) values. The results for the valuation function suggest that the estimated coefficients between the two sites are not transferable. However, the estimated WTP values can be transferred. The results suggest the suitability of using the CE approach in analysing benefit transfer if the objective is to transfer the WTP values rather than the valuation function. Copyright © 2014 John Wiley & Sons, Ltd.
Split‐calvarial osteopericranial flap for reconstruction following endoscopic anterior resection of cranial base
Authors: Engle Robert D. , Butrymowicz Anna , Peris‐Celda Maria , Kenning Tyler J. , Pinheiro‐Neto Carlos D. 
Published: October 2014

Objectives/HypothesisTo conceive and critically evaluate an adaptation of the split calvarial osteopericranial flap for reconstruction following endoscopic endonasal resection of the anterior skull base.Study DesignCadaveric anatomic study.MethodsFive cadavers were embalmed with methanol, and vasculature was injected with latex. Endoscopic endonasal resection of the anterior skull base was performed, followed by reconstruction with a unilateral osteopericranial flap and a contralateral conventional pericranial flap.ResultsRigid reconstruction was achieved in all specimens. Osteoplastic flap harvest was made more reliable by drilling the diploe below the graft with a curved bur. Dimensions of the bony flap were ideally shorter and wider than the defect, allowing for flap inset and rigid support by the orbits without compromise of the flap vascular supply. Endoscopic inset of the flap is feasible via nasion‐frontal osteotomy and inlay technique.ConclusionRigid anterior skull base reconstruction via split calvarial osteopericranial flap is adaptable to current endoscopic techniques. This provides more anatomic reconstruction than current methods and may lead to decreased complication rates following anterior skull base resection.Level of EvidenceN/A. Laryngoscope, 125:826–830, 2015
Effect of Solvent Type on High‐Temperature Thermal Gradient Interaction Chromatography of Polyethylene and Ethylene–1‐Octene Copolymers
Authors: Alghyamah Abdulaziz , Soares João B. P. 
Published: October 2014

The effects of the solvent type and operation conditions on the high‐temperature thermal gradient interaction chromatography (HT‐TGIC) of ethylene homopolymers, ethylene–1‐octene copolymers, and their blends are investigated. While the HT‐TGIC profiles of single polymers measured with 1,2,4‐trichlorobenzene (TCB) and chloronaphthalene (CN) are similar, they are always narrower when o‐dichlorobenzene (ODCB) is used, particularly for samples with lower 1‐octene fractions. Significant differences between the experimental and the calculated profiles of binary blends are observed with all three solvents, but better peak separation is seen when the ODCB is used. Having higher fractions of a 1‐octene‐poor component in the blend causes a more significant distortion of the shape expected for the peak from the component with the higher 1‐octene fraction. The effect of the molecular weight on HT‐TGIC profiles is also studied using samples with the same comonomer content and different molecular weights. Samples with low molecular weight have broader distributions and significant lower‐temperature tails, particularly when TCB is used. Chain crystallization after adsorption effects may also play a minor role for low‐comonomer samples. Finally, HT‐TGIC profiles are compared with their equivalent crystallization elution fractionation (CEF) profiles. The HT‐TGIC curves are broader than the equivalent CEF profiles, but these differences decrease as the comonomer content increases.
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Same Service, Different Ad Claims: The Moderating Role of Need for Cognition
Authors: Olsen Lars Erling , Samuelsen Bendik Meling , Gaustad Tarje 
Published: October 2014

ABSTRACTAdvertisers can choose among different ad claims when framing commercial messages about a new service offer. Two alternative ad claims are functional ad claims, focusing on product or service attributes and benefits, and experiential ad claims, focusing on future experiences with the product or service. This article proposes that different ad claims evoke different memory structures (semantic vs. episodic), and that different memory structures require different types of cognitive processing (system 1 vs. system 2). In two experiments using need for cognition as a dispositional trait of level of cognitive processing, this article shows that a new service entrant with experiential ad claims will be less favorably evaluated when consumers engage in more effortful, or system 2, processing, than when consumers engage in less effortful processing (system 1). For functional claims, however, the evaluation of the new service does not differ between the two types of cognitive processing (system 1 vs. system 2).
The Effects of Superstition on Choice and Latency
Authors: Ang Swee Hoon , Lai Wai Kit , Leong Siew Meng 
Published: October 2014

ABSTRACTThis study investigates the effects of superstition characteristics on choice and latency. Three dimensions of superstition are investigated—valence, accessibility, and relevance. Exposure to negative, accessible, or relevant superstition resulted in faster choices made. Superstitions that were negative or relevant led to choices favoring the auspicious product than superstitions that were positive or irrelevant. The auspicious product was also more likely to be chosen if it were associated with a superstition that was more accessible than less accessible. Findings also suggested that when superstition was relevant, the effects of a negative superstition were amplified than when superstition was irrelevant. Relevancy had less effect on positive superstition.
Brand Identification by Product Design: The Impact of Evaluation Mode and Familiarity
Authors: Herm Steffen , Möller Jana 
Published: October 2014

ABSTRACTDistinctive product design is a cornerstone of sustainable branding. Ideally, consumers will be able to identify brands by product design without seeing any logos. Once companies have established strong associations between product designs and brands in consumers’ minds, they seek protection against imitation as well as consistency in product lines. This research discusses methods for measuring consumers’ abilities to identify brands by product design. Study 1 shows that brand identification depends on the evaluation mode that the identification task evokes. Compared to a task that presents products side by side (comparative mode), participants are more likely to confuse copycats with an original design when they see only one product (noncomparative mode). Study 2 replicates findings of Study 1, demonstrating some robustness of effects across three countries (United States, Spain, Germany) and across three different product categories (beer, smartphones, cars). Study 2 further investigates how familiarity with the product influences brand identification. Although consumers with high (compared to low) familiarity are more likely to accurately identify an original product in a noncomparative evaluation mode, they are also more likely to confuse a copycat with the original product in this mode. Overall, this research extends knowledge about brand identification by product design and provides information brand managers may need for legal decisions in trademark or design patent cases. This research also provides information for brand managers making decisions regarding new product development, for example, product line extensions.