bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole‐Genome mRNA Expression Profiling Identifies Functional and Prognostic Signatures in Patients with Mesenchymal Glioblastoma Multiforme
Authors: Bao Zhao‐Shi , Zhang Chuan‐Bao , Wang Hong‐Jun , Yan Wei , Liu Yan‐Wei , Li Ming‐Yang , Zhang Wei 
Published: May 2013

SummaryBackgroundThe Cancer Genome Atlas (TCGA) has divided patients with glioblastoma multiforme (GBM) into four subtypes based on mRNA expression microarray. The mesenchymal subtype, with a larger proportion, is considered a more lethal one. Clinical outcome prediction is required to better guide more personalized treatment for these patients.AimsThe objective of this study was to identify a mRNA expression signature to improve outcome prediction for patients with mesenchymal GBM.ResultsFor signature identification and validation, we downloaded mRNA expression microarray data from TCGA as training set and data from Rembrandt and GSE16011 as validation set. Cox regression and risk‐score analysis were used to develop the 4 signatures, which were function and prognosis associated as revealed by Gene Ontology (GO) analysis and Gene Set Variation Analysis (GSVA). Patients who had high‐risk scores according to the signatures had poor overall survival compared with patients who had low‐risk scores.ConclusionsThe signatures were identified as risk predictors that patients who had a high‐risk score tended to have unfavorable outcome, demonstrating their potential for personalizing cancer management.
Association between EXOC3L2 rs597668 Polymorphism and Alzheimer's Disease
Authors: Shang Hong , Fu Jin , Zhang Xue‐Mei , Song Rong‐Rong , Wang Wei‐Zhi 
Published: May 2013

SummaryBackgroundEXOC3L2 gene rs597668 polymorphism was identified to be significantly associated with Alzheimer's disease (AD) in Caucasian population. However, recent studies reported consistent and inconsistent results in Caucasian and Asian populations.AimsIn order to assess this association, we performed a meta‐analysis of rs597668 polymorphism using RevMan (v.5.1) software.MethodsWe searched PubMed and Google scholar databases and selected 4 independent publications, which included 16 independent studies. We conducted sensitivity analysis and evaluated the publication bias. In the end, we calculated the odds ratio (OR) using fixed effect model (Mantel‐Haenszel).ResultsWe observed significant association between rs597668 polymorphism and AD using allele model (P = 0.006, OR = 1.09, 95% CI 1.03–1.16) and the dominant model (P = 0.008, OR = 1.11, 95% CI 1.03–1.21).Discussion and ConclusionsTo our knowledge, this is the first study that assesses the association between rs597668 polymorphism and AD by meta‐analysis. We believe that our findings will be very useful for future genetic studies in AD.
The results of surgery for colorectal hepatic metastases following expansion of the indications in 2005
Authors: Zhong Y. , Zhu D. , Liang L. , Ye Q. , Wei Y. , Ren L. , Pan X. , Fan J. , Xu J. , Qin X. 
Published: May 2013

AbstractAimSafety and survival were investigated in patients treated according to expanded surgical indications for colorectal hepatic metastases.MethodA retrospective analysis of all consecutive patients who underwent resection of colorectal hepatic metastases at Zhongshan Hospital from 2000 to 2010 was conducted. The patients were divided into two groups based on a change in the surgical indications introduced in 2005. Patients in Group I underwent hepatic surgery between 2000 and 2004 and those in Group II between 2005 and 2010. The clinicopathological data and survival rates of both groups were analysed.ResultsThere were 530 patients who underwent hepatic surgery between 2000 and 2010. After the expansion of surgical indications, the rate of surgical resection rose from 25.1 to 35.1% (P < 0.05). There was no significant difference in perioperative mortality (2.2% vs 0.9%) or morbidity (20.9% vs 29.8%). Recurrence occurred in 27.5% and 36.7% in Groups I and II, respectively, and 5‐year overall survival was 43% and 49%, respectively (not significant).ConclusionExpanding the indications for surgical resection of hepatic metastases increased the resection rate but had no significant effect on survival.
Colorectal neoplasia in long‐standing ulcerative colitis – a prospective study from a low‐prevalence area
Authors: Shivakumar B. M. , Lakshmankumar B. , Rao L. , Bhat G. , Suvarna D. , Pai C. G. 
Published: May 2013

AbstractAimDespite increasing recognition of ulcerative colitis (UC) in Asia in recent decades, reports on the occurrence of colorectal neoplasia (CRN) in UC are scarce and surveillance for this complication is not routinely practised in this region. We aimed to assess the outcome of a newly initiated pilot screening programme for screening CRN among UC patients in India.MethodIn this prospective study from an academic hospital setting, patients with UC at high risk of CRN were offered screening by magnifying chromocolonoscopy and the frequency of neoplastic lesions was assessed.ResultsTwenty‐nine (70.7%) of 41 eligible patients [a median age of 46 (interquartile range 36–54.5) years; 17 (58.6%) men] enrolled for surveillance; 41 colonoscopies were undertaken over 42 months. The median disease duration was 10 (interquartile range 7.5–14.5) years. Sixteen (55.1%) had extensive colitis. On initial screening, low‐grade dysplasia (LGD) was seen in five (17.2%) and high‐grade dysplasia (HGD) in three (10.3%). Of these three, one accepted proctocolectomy immediately, one underwent surgery for adenocarcinoma and one refused surgery. Twelve follow‐up colonoscopies in nine patients revealed three new LGD.ConclusionsHigh‐grade dysplasia and subsequent adenocarcinoma can be detected with careful follow‐up in Indian patients with long‐standing UC but acceptance of surveillance and subsequent therapy are suboptimal. We found evidence that screening and surveillance programmes are useful for detecting neoplasias in UC, and need to be customized for this region.
Spent green tea leaves for decolourisation of raw textile industry wastewater
Authors: Weng Chih‐Huang , Lin Yao‐Tung , Chen Yen‐Jung , Sharma Yogesh Chandra 
Published: May 2013

True colour measurements of wastewater samples, in terms of American Dye Manufacturers' Institute values, were used to evaluate the effectiveness of decolourisation of textile wastewater using spent green tea leaf powder waste. Raw wastewater samples with a true colour of 868 ADMI were used in the batch adsorption experiments. Results revealed that the true colour removal efficiency of the raw textile wastewater was high in acidic solution and at high temperature, indicating an endothermic nature of the system. The major functional groups of the green tea leaf powder waste involved in adsorption were identified by Fourier Transform‐infrared spectroscopy analysis. Adsorption kinetic data were modelled using the modified Freundlich and intraparticle diffusion kinetics equations. Fitting results of the Langmuir adsorption isotherm showed that the adsorption capacity of the green tea leaf powder waste was 775 ADMI g−1, which is higher than that of powder activated carbon (526 ADMI g−1). The low activation energy values (13.9 kJ mol−1) suggested that adsorption was governed by a diffusion process and the reaction involved a physisorption mechanism. From the perspectives of waste utilisation, remarkable colour adsorption capacity and inexpensive and abundant availability, green tea leaf waste is an attractive alternative for decolourisaton of textile wastewater. Because high temperature favours colour removal, green tea leaf powder can be directly applied in raw textile wastewater treatment.
Thirty days of resveratrol supplementation does not affect postprandial incretin hormone responses, but suppresses postprandial glucagon in obese subjects
Authors: Knop F. K. , Konings E. , Timmers S. , Schrauwen P. , Holst J. J. , Blaak E. E. 
Published: May 2013

AbstractAimsResveratrol, a natural polyphenolic compound produced by various plants (e.g. red grapes) and found in red wine, has glucose‐lowering effects in humans and rodent models of obesity and/or diabetes. The mechanisms behind these effects have been suggested to include resveratrol‐induced secretion of the gut incretin hormone glucagon‐like peptide‐1. We investigated postprandial incretin hormone and glucagon responses in obese human subjects before and after 30 days of resveratrol supplementation.MethodsPostprandial plasma responses of the incretin hormones glucagon‐like peptide‐1 and glucose‐dependent insulinotropic polypeptide and glucagon were evaluated in 10 obese men [subjects characteristics (mean ± standard error of the mean): age 52 ± 2 years; BMI 32 ± 1 kg/m2, fasting plasma glucose 5.5 ± 0.1 mmol/l] who had been given a dietary supplement of resveratrol (Resvida® 150 mg/day) or placebo for 30 days in a randomized, double‐blind, crossover design with a 4‐week washout period. At the end of each intervention period a standardized meal test (without co‐administration of resveratrol) was performed.ResultsResveratrol supplementation had no impact on fasting plasma concentrations or postprandial plasma responses (area under curve values) of glucose‐dependent insulinotropic polypeptide (11.2 ± 2.1 vs. 11.8 ± 2.2 pmol/l, P = 0.87; 17.0 ± 2.2 vs. 14.8 ± 1.6 min × nmol/l, P = 0.20) or glucagon‐like peptide‐1 (15.4 ± 1.0 vs. 15.2 ± 0.9 pmol/l, P = 0.84; 5.6 ± 0.4 vs. 5.7 ± 0.3 min × nmol/l, P = 0.73). Resveratrol supplementation significantly suppressed postprandial glucagon responses (4.4 ± 0.4 vs. 3.9 ± 0.4 min × nmol/l, P = 0.01) without affecting fasting glucagon levels (15.2 ± 2.2 vs. 14.5 ± 1.5 pmol/l, P = 0.56).ConclusionsOur data suggest that 30 days of resveratrol supplementation does not affect fasting or postprandial incretin hormone plasma levels in obese humans, but suppresses postprandial glucagon responses.
The Role of Three‐Dimensional Echocardiography in Diagnosis and Management of Ruptured Sinus of Valsalva Aneurysm
Authors: Vatankulu Mehmet Akif , Tasal Abdurrahman , Erdogan Ercan , Sonmez Osman , Goktekin Omer 
Published: May 2013

Mini‐AbstractA 29‐year‐old man was admitted to our outpatient clinic with exertional dyspnea. Transesophageal echocardiography (TEE) revealed a ruptured sinus of Valsalva aneurysm into the right ventricle. Real time three‐dimensional (3D) TEE, used to visualize the relationship between the rupture, the aortic valve, and the right ventricle, revealed the precise shape, size, and location of the defect. The defect was closed using a 16/10 mm Amplatzer duct occluder under real time 3DTEE guidance. 3DTEE provided more accurate delineation of the defect location and orientation than other imaging techniques and may aid in the selection of an appropriate device for closure.
Multimeric and differential binding of CIN85/CD2AP with two atypical proline‐rich sequences from CD2 and Cbl‐b*
Authors: Ceregido M. Angeles , Garcia‐Pino Abel , Ortega‐Roldan Jose L. , Casares Salvador , López Mayorga Obdulio , Bravo Jeronimo , van Nuland Nico A. J. , Azuaga Ana I. 
Published: May 2013

The CD2AP (CD2‐associated protein) and CIN85 (Cbl‐interacting protein of 85 kDa) adaptor proteins each employ three Src homology 3 (SH3) domains to cluster protein partners and ensure efficient signal transduction and down‐regulation of tyrosine kinase receptors. Using NMR, isothermal titration calorimetry and small‐angle X‐ray scattering methods, we have characterized several binding modes of the N‐terminal SH3 domain (SH3A) of CD2AP and CIN85 with two natural atypical proline‐rich regions in CD2 (cluster of differentiation 2) and Cbl‐b (Casitas B‐lineage lymphoma), and compared these data with previous studies and published crystal structures. Our experiments show that the CD2AP‐SH3A domain forms a type II dimer with CD2 and both type I and type II dimeric complexes with Cbl‐b. Like CD2AP, the CIN85‐SH3A domain forms a type II complex with CD2, but a trimeric complex with Cbl‐b, whereby the type I and II interactions take place at the same time. Together, these results explain how multiple interactions among similar SH3 domains and ligands produce a high degree of diversity in tyrosine kinase, cell adhesion or T‐cell signaling pathways.Structured digital abstract

CIN85 and Cbl-b bind by nuclear magnetic resonance (View Interaction: 1, 2)

CD2 and CIN85 bind by nuclear magnetic resonance (View interaction)

CD2 and CD2AP bind by nuclear magnetic resonance (View interaction)

Cbl-b and CD2AP bind by nuclear magnetic resonance (View Interaction: 1, 2)

Cbl-b and CIN85 bind by isothermal titration calorimetry (View Interaction: 1, 2, 3, 4)

CIN85 and Cbl-b bind by X-ray scattering (View Interaction: 1, 2)

CIN85 and CD2 bind by isothermal titration calorimetry (View interaction)

CD2 and CD2AP bind by X-ray scattering (View interaction)

CD2AP and CD2 bind by isothermal titration calorimetry (View interaction)

CD2 and CIN85 bind by X-ray scattering (View interaction)

CD2AP and Cbl-b bind by isothermal titration calorimetry (View Interaction: 1, 2, 3, 4, 5)
Are silicate‐rich inclusions in spodumene crystallized aliquots of boundary layer melt?
Authors: Anderson A. J. 
Published: May 2013

AbstractThe constitutional zone refining model for the internal crystallization of granitic pegmatites stipulates that a highly fluxed and incompatible element‐enriched boundary layer melt evolves at the surface of crystal growth fronts in undercooled granitic melts. It has been argued that silicate‐rich inclusions in primary spodumene and petalite from the Tanco pegmatite are the crystallized products of an entrapped boundary layer melt. However, inclusions of identical character and composition to those in primary spodumene are also abundant in the spodumene component of SQI (spodumene + quartz intergrowths) that formed by the isochemical breakdown of petalite. Both textural types of spodumene at Tanco host fluid inclusion assemblages that display highly variable proportions of solid phases (dominantly cookeite, quartz, and zabuyelite) and a low salinity (approximately 6 wt% NaCl equivalent) aqueous fluid. The average major element content of three fluid inclusion assemblages is approximately Li2O (3 wt%), Al2O3 (11 wt%), SiO2 (30 wt%), CO2 (3 wt%) and H2O (53 wt%). The entrapment of these fluid inclusions is temporally and spatially unrelated to the flux‐rich melt that may have developed at the primary crystallization front of the pegmatite. Petrographic evidence and the mineralogical and chemical composition of spodumene‐hosted inclusions suggest that inclusions in spodumene are the products of hydrothermal dissolution of the host and the precipitation of quartz, cookeite, and zabuyelite prior to and during necking.
“Metakaolin‐Slag‐Clinker Blends.” The Role of Na+ or K+ as Alkaline Activators of Theses Ternary Blends
Authors: Fernández‐Jiménez Ana , Zibouche Fatima , Boudissa Nassima , García‐Lodeiro Ines , Abadlia Mohamed Tahar , Palomo Angel 
Published: May 2013

This article reports on research into the use of solid alkalis (Na2CO3 and K2CO3) as activators to obtain hybrid cement (cement whose hydration generates a mix of C–A–S–H and (N,C)–A–S–H gels) from a blend of 20% clínker + 40% blast furnace slag + 40% metakaolin. More specifically, the study aimed to determine the effect of activator dosage (5 and 8 wt%) and type of alkaline cation (Na+ or K+) on the 2‐ and 28‐d mechanical strength of the end materials. The findings showed that the highest mechanical strength values were obtained with 5% Na2CO3. According to the XRD, NMR, and SEM/EDX analyses conducted on the reaction products, the alkalinity and solubilized chemical species generated by adding 5% Na2CO3 to the system yielded a mix of (N,C)–A–S–H and C–A–S–H cementitious gels as the main reaction products. The secondary reaction products included metastable (3CaO·Al2O3·CaCO3·11H2O‐type) carboaluminates that evolved into the calcite or vaterite forms of calcium carbonate. When K2CO3 was used (instead of Na2CO3), a (3CaO·Al2O3·0.5Ca(OH)2·0.5CaCO3·11H2O‐type) hemicarboaluminate also formed. The study also revealed that Na+ favors coagulation/precipitation more effectively than K+, generating gels with a wider range of Qn species.
Directional protein secretion by the retinal pigment epithelium: roles in retinal health and the development of age‐related macular degeneration
Authors: Kay Paul , Yang Yit C. , Paraoan Luminita 
Published: May 2013

AbstractThe structural and functional integrity of the retinal pigment epithelium (RPE) is fundamental for maintaining the function of the neuroretina. These specialized cells form a polarized monolayer that acts as the retinal–blood barrier, separating two distinct environments with highly specialized functions: photoreceptors of the neuroretina at the apical side and Bruch's membrane/highly vascularized choriocapillaris at the basal side. The polarized nature of the RPE is essential for the health of these two regions, not only in nutrient and waste transport but also in the synthesis and directional secretion of proteins required in maintaining retinal homoeostasis and function. Although multiple malfunctions within the RPE cells have been associated with development of age‐related macular degeneration (AMD), the leading cause of legal blindness, clear causative processes have not yet been conclusively characterized at the molecular and cellular level. This article focuses on the involvement of directionally secreted RPE proteins in normal functioning of the retina and on the potential association of incorrect RPE protein secretion with development of AMD. Understanding the importance of RPE polarity and the correct secretion of essential structural and regulatory components emerge as critical factors for the development of novel therapeutic strategies targeting AMD.
In vitro chipping behaviour of all‐ceramic crowns with a zirconia framework and feldspathic veneering: comparison of CAD/CAM‐produced veneer with manually layered veneer
Authors: Schmitter M. , Mueller D. , Rues S. 
Published: May 2013

SummaryThe purpose of this in vitro study was to assess the breaking load of zirconia‐based crowns veneered with either CAD/CAM‐produced or manually layered feldspathic ceramic. Thirty‐two identical zirconia frameworks (Sirona inCoris ZI, mono L F1), 0·6 mm thick with an anatomically shaped occlusal area, were constructed (Sirona inLab 3.80). Sixteen of the crowns were then veneered by the use of CAD/CAM‐fabricated feldspathic ceramic (CEREC Bloc, Sirona) and 16 by the use of hand‐layered ceramic. The CAD/CAM‐manufactured veneer was attached to the frameworks by the use of Panavia 2.0 (Kuraray). Half of the specimens were loaded until failure without artificial ageing; the other half of the specimens underwent thermal cycling and cyclic loading (1·2 million chewing cycles, force magnitude Fmax = 108 N) before the assessment of the ultimate load. To investigate the new technique further, finite element (FE) computations were conducted on the basis of the original geometry. Statistical assessment was made by the use of non‐parametric tests. Initial breaking load was significantly higher in the hand‐layered group than in the CAD/CAM group (mean: 1165·86 N versus 395·45 N). During chewing simulation, however, 87·5% (7/8) of the crowns in the hand‐layered group failed, whereas no crown in the CAD/CAM group failed. The CAD/CAM‐produced veneer was significantly less sensitive to ageing than the hand‐layered veneer.
Assessment of the quality of reporting in abstracts of systematic reviews with meta‐analyses in periodontology and implant dentistry
Authors: Faggion C. M. , Liu J. , Huda F. , Atieh M. 
Published: May 2013

BackgroundProper scientific reporting is necessary to ensure the correct interpretation of study results by readers. The main objective of this study was to assess the quality of reporting in abstracts of systematic reviews (SRs) with meta‐analyses in periodontology and implant dentistry. Differences in reporting of abstracts in Cochrane and paper‐based reviews were also assessed.MethodsThe PubMed electronic database and the Cochrane database for SRs were searched on November 11, 2012, independently and in duplicate, for SRs with meta‐analyses related to interventions in periodontology and implant dentistry. Assessment of the quality of reporting was performed independently and in duplicate, taking into account items related to the effect direction, numerical estimates of effect size, measures of precision, probability and consistency.ResultsWe initially screened 433 papers and included 146 (127 paper‐based and 19 Cochrane reviews, respectively). The direction of evidence was reported in two‐thirds of the abstracts while strength of evidence and measure of precision (i.e., confidence interval) were reported in less than half the selected abstracts. Measures of consistency such as I2 statistics were reported in only 5% of the selected sample of abstracts. Cochrane abstracts reported the limitations of evidence and precision better than paper‐based ones. Two items (“meta‐analysis” in title and abstract, respectively), were nevertheless better reported in paper‐based abstracts.ConclusionAbstracts of SRs with meta‐analyses in periodontology and implant dentistry currently have no uniform standard of reporting, which may hinder readers’ understanding of study outcomes.
The relationship between root concavities in first premolars and chronic periodontitis
Authors: Zhao H. , Wang H. , Pan Y. , Pan C. , Jin X. 
Published: May 2013

ObjectiveTo evaluate the significance of first premolar root concavity on clinical indices of chronic periodontal disease and alveolar bone defects.MethodsThree‐dimensional reconstruction by cone beam computed tomography was used to observe root surface anatomy and the type of alveolar bone defect seen in the mesial and distal sites of 272 first premolars from 99 patients who had presented with chronic periodontitis. Periodontal clinical indicators at each site were measured using a Florida Probe Corporation (Gainesville, FL, USA).ResultsThe incidence of mesial and distal root concavities of the maxillary first premolars was 100% and 39.3% respectively, and in the mandibular, the incidence was 42.5% and 31.3% respectively. The distributions of the different types of concavities in terms of both age and gender of the patients were not statistically significant. The mean probing depth and clinical attachment loss of the first premolars with root concavities were significantly higher than those without concavity (p < 0.05). Plaque accumulation was significantly different in the premolars with/without root concavities (p < 0.001). The type of alveolar bone defects with concavities was significantly different from those without concavities (p < 0.05). Ramp shape bone defects were dominant for teeth without concavities, while crater shape was seen for teeth with concavities.ConclusionRoot concavities of the first premolars were associated with periodontal disease, and the type of interproximal alveolar bone defect. Root concavities may be important in contributing to local periodontal disease of the first premolars.