bluuebunny/crossref_metadata_2025_split huggingface dataset:
Programmed Death-1 Shapes Memory Phenotype CD8 T Cell Subsets in a Cell-Intrinsic Manner
Authors: Charlton Joanna J. , Chatzidakis Ioannis , Tsoukatou Debbie , Boumpas Dimitrios T. , Garinis George A. , Mamalaki Clio 
Published: May 2013

Abstract
Memory phenotype T cells, found in unimmunized mice, display phenotypic and functional traits of memory cells and provide essential protection against infections, playing a role in both innate and adaptive immune responses. Mechanisms governing homeostasis of these memory phenotype T cells remain ill-defined. In this study, we reveal a crucial role of the negative costimulator programmed death-1 (PD-1) in regulating developmental fates of memory phenotype cells. Thus, in lymphoid organs and tissues of PD-1 knockout (KO) mice a marked accumulation of functional effector memory (TEM) phenotype CD8 T cells was observed. TEM phenotype cells from PD-1 KO mice exhibit decreased proliferation but increased survival potential. These cells could produce effector molecules constitutively, in response to phorbol esters or through bystander activation by innate stimuli. Similarly, in lymphopenia-induced proliferating CD8 T cells, whereby normally naive T cells acquire a memory phenotype, skewing toward a TEM phenotype was prominent in the absence of PD-1. Acquisition of the TEM phenotype was a CD8 T cell–intrinsic phenomenon as demonstrated by mixed bone marrow transfer experiments. Importantly, adoptively transferred PD-1 KO CD8 central memory T (TCM) cells converted into the TEM phenotype, indicating that PD-1 sets a major checkpoint in the TCM to TEM phenotype differentiation process. This was reflected by distinct patterns of gene expression of PD-1 KO TCM phenotype cells revealed by global transcriptional analysis. Additionally, adoptively transferred PD-1 KO TEM phenotype cells converted to a lesser degree to a TCM phenotype. Collectively, these data suggest that PD-1 shapes memory phenotype CD8 T cell subsets.
Proresolving and Tissue-Protective Actions of Annexin A1–Based Cleavage-Resistant Peptides Are Mediated by Formyl Peptide Receptor 2/Lipoxin A4 Receptor
Authors: Dalli Jesmond , Consalvo Angelo P. , Ray Vicki , Di Filippo Clara , D’Amico Michele , Mehta Nozer , Perretti Mauro 
Published: May 2013

Abstract
Endogenous mechanisms regulating the host response during inflammation resolution are critical in ensuring disposal of noxious stimuli and return to homeostasis. In this article, we engineered novel Annexin A1 (AnxA1)–based peptides, AnxA12–50, that displayed specific binding to the AnxA1 receptor (formyl peptide receptor 2/Lipoxin A4 receptor [FPR2/ALX]; IC50 ∼4 nM). Intravenous administration of AnxA12–50 markedly reduced (&gt;60%) leukocyte adhesion to postcapillary venules in wild type and Fpr1−/−, but not Fpr2/Alx−/−, mice. Generation of a metabolically stable form of this peptide (CR-AnxA12–50), engineered by substituting a cleavage site shared by human proteinase 3 and neutrophil elastase, yielded an agonist that was resistant to neutrophil-mediated cleavage and displayed enhanced proresolving actions: accelerated resolution of self-limited inflammation and enhanced macrophage efferocytosis after sterile injury, when compared with AnxA12–50. These actions were retained with human primary leukocytes where CR-AnxA12–50 decreased neutrophil–endothelial interactions (∼25–45%), and stimulated neutrophil apoptosis and macrophage efferocytosis (∼45%). In murine cardiac ischemia/reperfusion injury, CR-AnxA12–50 elicited tissue-protective actions reducing infarct size (∼60%) and incidence of 24-h death. These results identify AnxA12–50 and CR-AnxA12–50 as FPR2/ALX agonists that harness the proresolving actions of AnxA1, and thus may represent therapeutic tools for treatment of inflammatory conditions.
Profound Impairment of Adaptive Immune Responses by Alkylating Chemotherapy
Authors: Litterman Adam J. , Zellmer David M. , Grinnen Karen L. , Hunt Matthew A. , Dudek Arkadiusz Z. , Salazar Andres M. , Ohlfest John R. 
Published: May 2013

Abstract
Overall, cancer vaccines have had a record of failure as an adjuvant therapy for malignancies that are treated with alkylating chemotherapy, and the contribution of standard treatment to that failure remains unclear. Vaccines aim to harness the proliferative potential of the immune system by expanding a small number of tumor-specific lymphocytes into a large number of antitumor effectors. Clinical trials are often conducted after treatment with alkylating chemotherapy, given either as standard therapy or for immunomodulatory effect. There is mounting evidence for synergy between chemotherapy and adoptive immunotherapy or vaccination against self-Ags; however, the impact of chemotherapy on lymphocytes primed against tumor neoantigens remains poorly defined. We report that clinically relevant dosages of standard alkylating chemotherapies, such as temozolomide and cyclophosphamide, significantly inhibit the proliferative abilities of lymphocytes in mice. This proliferative impairment was long-lasting and led to quantitative and qualitative defects in B and T cell responses to neoantigen vaccines. High-affinity responder lymphocytes receiving the strongest proliferative signals from vaccines experienced the greatest DNA damage responses, skewing the response toward lower-affinity responders with inferior functional characteristics. Together, these defects lead to inferior efficacy and overall survival in murine tumor models treated by neoantigen vaccines. These results suggest that clinical protocols for cancer vaccines should be designed to avoid exposing responder lymphocytes to alkylating chemotherapy.
MicroRNA-155 Confers Encephalogenic Potential to Th17 Cells by Promoting Effector Gene Expression
Authors: Hu Ruozhen , Huffaker Thomas B. , Kagele Dominique A. , Runtsch Marah C. , Bake Erin , Chaudhuri Aadel A. , Round June L. , O’Connell Ryan M. 
Published: May 2013

Abstract
Th17 cells are central to the pathogenesis of autoimmune disease, and recently specific noncoding microRNAs have been shown to regulate their development. However, it remains unclear whether microRNAs are also involved in modulating Th17 cell effector functions. Consequently, we examined the role of miR-155 in differentiated Th17 cells during their induction of experimental autoimmune encephalomyelitis. Using adoptive transfer experiments, we found that highly purified, myelin oligodendrocyte glycoprotein Ag-specific Th17 cells lacking miR-155 were defective in their capacity to cause experimental autoimmune encephalomyelitis. Gene expression profiling of purified miR-155−/−IL-17F+ Th17 cells identified a subset of effector genes that are dependent on miR-155 for their proper expression through a mechanism involving repression of the transcription factor Ets1. Among the genes reduced in the absence of miR-155 was IL-23R, resulting in miR-155−/− Th17 cells being hyporesponsive to IL-23. Taken together, our study demonstrates a critical role for miR-155 in Th17 cells as they unleash autoimmune inflammation and finds that this occurs through a signaling network involving miR-155, Ets1, and the clinically relevant IL-23–IL-23R pathway.
Protective Role of the Inflammatory CCR2/CCL2 Chemokine Pathway through Recruitment of Type 1 Cytotoxic γδ T Lymphocytes to Tumor Beds
Authors: Lança Telma , Costa Maria Fernanda , Gonçalves-Sousa Natacha , Rei Margarida , Grosso Ana Rita , Penido Carmen , Silva-Santos Bruno 
Published: May 2013

Abstract
Tumor-infiltrating lymphocytes (TILs) are important prognostic factors in cancer progression and key players in cancer immunotherapy. Although γδ T lymphocytes can target a diversity of tumor cell types, their clinical manipulation is hampered by our limited knowledge of the molecular cues that determine γδ T cell migration toward tumors in vivo. In this study we set out to identify the chemotactic signals that orchestrate tumor infiltration by γδ T cells. We have used the preclinical transplantable B16 melanoma model to profile chemokines in tumor lesions and assess their impact on γδ TIL recruitment in vivo. We show that the inflammatory chemokine CCL2 and its receptor CCR2 are necessary for the accumulation of γδ TILs in B16 lesions, where they produce IFN-γ and display potent cytotoxic functions. Moreover, CCL2 directed γδ T cell migration in vitro toward tumor extracts, which was abrogated by anti-CCL2 neutralizing Abs. Strikingly, the lack of γδ TILs in TCRδ-deficient but also in CCR2-deficient mice enhanced tumor growth in vivo, thus revealing an unanticipated protective role for CCR2/CCL2 through the recruitment of γδ T cells. Importantly, we demonstrate that human Vδ1 T cells, but not their Vδ2 counterparts, express CCR2 and migrate to CCL2, whose expression is strongly deregulated in multiple human tumors of diverse origin, such as lung, prostate, liver, or breast cancer. This work identifies a novel protective role for CCL2/CCR2 in the tumor microenvironment, while opening new perspectives for modulation of human Vδ1 T cells in cancer immunotherapy.
Allelic Variation in KIR2DL3 Generates a KIR2DL2-like Receptor with Increased Binding to its HLA-C Ligand
Authors: Frazier William R. , Steiner Noriko , Hou Lihua , Dakshanamurthy Sivanesan , Hurley Carolyn Katovich 
Published: May 2013

AbstractAlthough extensive homology exists between their extracellular domains, NK cell inhibitory receptors killer Ig-like receptor (KIR) 2DL2*001 and KIR2DL3*001 have previously been shown to differ substantially in their HLA-C binding avidity. To explore the largely uncharacterized impact of allelic diversity, the most common KIR2DL2/3 allelic products in European American and African American populations were evaluated for surface expression and binding affinity to their HLA-C group 1 and 2 ligands. Although no significant differences in the degree of cell membrane localization were detected in a transfected human NKL cell line by flow cytometry, surface plasmon resonance and KIR binding to a panel of HLA allotypes demonstrated that KIR2DL3*005 differed significantly from other KIR2DL3 allelic products in its ability to bind HLA-C. The increased affinity and avidity of KIR2DL3*005 for its ligand was also demonstrated to have a larger impact on the inhibition of IFN-γ production by the human KHYG-1 NK cell line compared with KIR2DL3*001, a low-affinity allelic product. Site-directed mutagenesis established that the combination of arginine at residue 11 and glutamic acid at residue 35 in KIR2DL3*005 were critical to the observed phenotype. Although these residues are distal to the KIR/HLA-C interface, molecular modeling suggests that alteration in the interdomain hinge angle of KIR2DL3*005 toward that found in KIR2DL2*001, another strong receptor of the KIR2DL2/3 family, may be the cause of this increased affinity. The regain of inhibitory capacity by KIR2DL3*005 suggests that the rapidly evolving KIR locus may be responding to relatively recent selective pressures placed upon certain human populations.
Bilateral Tarsal Coalition in a National Collegiate Athletic Association Division I Basketball Player: A Case Report
Authors: Suits Julie M. , Oliver Gretchen D. 
Published: May 2013

ObjectiveTo present a case of bilateral subtalar joint coalition in a National Collegiate Athletic Association Division I basketball player and the treatment plan that was used to manage the coalition from the beginning of conference play through the postseason.BackgroundA 20-year-old male basketball athlete (height = 182.8 cm, mass = 83.4 kg) presented with bilateral subtalar joint tarsal coalition that became symptomatic in 2006 and resulted in constant pain with any form of activity.Differential DiagnosisTraumatic injury of the talocalcaneal joint.TreatmentNonsurgical intervention of conservative therapy was elected.UniquenessLess than 13% of the overall population is affected with tarsal coalition, so it is safe to assume that very few athletes competing at the collegiate or elite level suffer from this condition. This is the first report in the literature to document conservative manual therapies used to manage the symptoms of subtalar joint tarsal coalition in a Division I basketball player.ConclusionsAfter the intensive treatment program for tarsal coalition was implemented, the patient experienced pain relief and was able to continue to compete at a competitive level. This case represents the need to further explore and document a conservative treatment protocol for tarsal coalition.
Red Light and the Sleep Quality and Endurance Performance of Chinese Female Basketball Players
Authors: Zhao Jiexiu , Tian Ye , Nie Jinlei , Xu Jincheng , Liu Dongsen 
Published: May 2013

Context
Good sleep is an important recovery method for prevention and treatment of overtraining in sport practice. Whether sleep is regulated by melatonin after red-light irradiation in athletes is unknown.

Objective
To determine the effect of red light on sleep quality and endurance performance of Chinese female basketball players.

Design
Cohort study.

Setting
Athletic training facility of the Chinese People's Liberation Army and research laboratory of the China Institute of Sport Science.

Patients or Other Participants
Twenty athletes of the Chinese People's Liberation Army team (age = 18.60 ± 3.60 years) took part in the study. Participants were divided into red-light treatment (n = 10) and placebo (n = 10) groups.

Intervention(s)
The red-light treatment participants received 30 minutes of irradiation from a red-light therapy instrument every night for 14 days. The placebo group did not receive light illumination.

Main Outcome Measure(s)
The Pittsburgh Sleep Quality Index (PSQI) questionnaire was completed, serum melatonin was assessed, and 12-minute run was performed at preintervention (baseline) and postintervention (14 days).

Results
The 14-day whole-body irradiation with red-light treatment improved the sleep, serum melatonin level, and endurance performance of the elite female basketball players (P &lt; .05). We found a correlation between changes in global Pittsburgh Sleep Quality Index and serum melatonin levels (r = −0.695, P = .006).

Conclusions
Our study confirmed the effectiveness of body irradiation with red light in improving the quality of sleep of elite female basketball players and offered a nonpharmacologic and noninvasive therapy to prevent sleep disorders after training.
Compliance With Neuromuscular Training and Anterior Cruciate Ligament Injury Risk Reduction in Female Athletes: A Meta-Analysis
Authors: Sugimoto Dai , Myer Gregory D. , Bush Heather M. , Klugman Maddie F. , McKeon Jennifer M. Medina , Hewett Timothy E. 
Published: May 2013

Context
No consensus exists about the influence of compliance with neuromuscular training programs on reduction of the risk of anterior cruciate ligament (ACL) injury.

Objective
To systematically review and synthesize the published literature to determine if compliance with neuromuscular training is associated with reduced incidence of ACL injury in young female athletes.

Data Sources
We searched PubMed, SPORTDiscus, CINAHL, and MEDLINE for articles published from 1995 to 2010 using the key words anterior cruciate ligament prevention, ACL prevention, knee prevention, prospective knee prevention, neuromuscular training, and neuromuscular intervention.

Study Selection
Criteria for inclusion required that (1) the number of ACL injuries was reported, (2) a neuromuscular training program was used, (3) females were included as participants, (4) the study design was prospective and controlled, and (5) compliance data for the neuromuscular training program were provided.

Data Extraction
Extracted data included the number of ACL injuries, total number of participants per group, observation time period, number of participants who completed each session, number of sessions completed by an entire team, and number of total sessions. Attendance was calculated as the number of participants who completed each session converted into a percentage of the total number of participants. Intervention completion was calculated as the number of sessions completed by an entire team converted into a percentage of the total number of training sessions. These data were used to calculate an overall rate of compliance.

Data Synthesis
Six of 205 identified studies were included. Incidence rates of ACL injury were lower in studies with high rates of compliance with neuromuscular training than in studies with low compliance rates (incidence rate ratio = 0.27 [95% confidence interval = 0.07, 0.80]). Tertile analysis indicated rates of ACL injury incidence were lower in studies with high compliance rates than in studies with moderate and low compliance rates (incidence rate ratio = 0.18 [95% confidence interval = 0.02, 0.77]).

Conclusions
A potential inverse dose-response relationship exists between compliance with neuromuscular training and incidence of ACL injury. Attending and completing recommended neuromuscular sessions appears to be an important factor for preventing ACL injuries.
A cytological analysis of the oral mucosa adjacent to orthodontic devices
Authors: Sampaio Mei Raul Magnoler , Soares de Lima Antônio Adilson , Filho Jorge César Borges Leão , Tanaka Orlando Motohiro , Filho Odilon Guariza , Camargo Elisa Souza 
Published: May 2013

Abstract
Objectives: The objective of this study, was to evaluate the cytometry and cytomorphology of oral mucosa epithelial cells adjacent to orthodontic accessories using liquid-based exfoliative cytology. Materials and Methods: We placed metallic brackets on the upper pre-molars with stainless steel wires and metal or elastic ties on 20 people of both sexes, with an average age of 22.7 years. We evaluated nucleus area (NA), cytoplasm area (CA), and NA/CA ratio before installation (T0, baseline), 30 days after installation (T1), and 30 days after the accessories were removed (T2). Results: We verified a reduction in NA and an increase in CA with both types of ties at T1 (P<0.05 vs. T0), with a tendency to return to baseline values at T2 (P<0.05 vs. T0). At T1 and T2 we observed a predominance of surface cells over subsurface cells. Conclusions: The orthodontic appliance causes adaptive cell changes characterized by an increased level of keratinization of the oral mucosa, with a tendency to return to the normal state after removal of the mechanical stimulus of the orthodontic devices.
Comparative analysis of adhesive failure of orthodontic resins: An in vitro mechanical test with the finite element method
Authors: Avila Ana Leticia Rocha , Hecke Mildred Ballin , de Oliveira Franco Ana Paula Gebert , Vasco Marco Antonio Amorin , Oliveira Dauro Douglas , Tanaka Orlando Motohiro 
Published: May 2013

Abstract
Objective: The purpose of this study was to validate finite element (FE) method as a reliable adhesive shear strength test method by investigating and comparing the results from in vitro mechanical tests and 3-D FE simulations. Materials and Methods: Four groups of teeth (n=15) using Transbond XT (3M Unitek, Monrovia, CA) and Enlight Ormco (Glendora, CA) with metallic and ceramic brackets (Twin-Edge and InVu, TP Orthodontics, Inc., La Porte, IN) were obtained and submitted to shear bond strength tests. Subsequently, an equivalent geometric model was subjected to FE modeling analysis. ANOVA tests indicated a statistically significant difference (P<0.05) between the shear bond strength of the two bracket types regardless of the resin, and there was no interaction between the resin and bracket type. Results: FE analysis showed the stress distribution in the adhesive layer and revealed an increased stress distribution in the ceramic brackets. These results were consistent with in vitro detachment experiments. Conclusions: This study establishes that FE sub-modeling can be used to simulate adhesive resistance.
Comparison of two different restoration materials and two different implant designs of implant-supported fixed cantilevered prostheses: A 3D finite element analysis
Authors: Culhaoglu Ahmet Kursad , Ozkir Serhat Emre , Celik Gozde , Terzioglu Hakan 
Published: May 2013

Abstract
Purpose: A key factor for the success or failure of a dental implant is the manner of stresses transferred to the surrounding bone. Parallel to this situation, cantilever extensions where it is not possible to place another implant, would cause greater stress and it should be avoided if possible. Manufacturers and clinicians try to develop new implant designs and superstructure materials to reduce the stresses around the implant and supporting bone tissue. This study analyzed the influence of superstructure materials and implant designs on stress distribution around dental implants supporting cantilever restoration under loading conditions. Materials and Methods: Three-dimensional finite element models of a 3-unit cantilever bridge were subjected to 150 N occlusal load to evaluate two different superstructure materials (conventional metal ceramic, fiber reinforced composite) and two different implant designs, cylinder type (Institut Straumann AG, Waldenburg, Switzerland) and cylinder type with micro threads around implant neck (Astra Tech AB, Mölndal, Sweden). To evaluate the distribution of stresses within the bone surrounding the implants, 3-dimensional finite element analysis was conducted using four mathematical models of unilateral 3-unit cantilever fixed partial dentures supported by two implants. Results: The stress distribution patterns and stress values were similar and stress concentrations were similar in both restoration materials. The highest stress concentrations were around the adjacent ITI implant, which supports the conventional metal porcelain restoration. Conclusions: Although, there was no significant difference in stress distribution between fiber reinforced composite (FRC) and conventional metal porcelain, stress values were lower in FRC restorations. The Astra-Tech micro-thread design reduced the stress that was distributed throughout the implant body, but it should be noted that the peak stress was still present on the implant neck.
Interdisciplinary approach to treating a patient with amelogenesis imperfecta: A clinical report
Authors: Sari Mustafa Erhan , Arici Nursel , Ozmen Bilal 
Published: May 2013

AbstractAmelogenesis imperfecta is a rare hereditary condition in which enamel structure without evidence of systemic disorders. Amelogenesis imperfecta is a disorder, requiring treatment due to esthetic, functional and related psychosocial problems. This article represents, a patient that referred to our clinic suffering from amelogenesis imperfect who successfully overcame the esthetic, functional and psychosocial problems by receiving multidisciplinary team rehabilitation.
Maxillary ameloblastoma extending into the maxillary sinus
Authors: Dwivedi Nidhi , Raj Vineet , Chandra Shaleen , Agarwal Akhil 
Published: May 2013

AbstractAmeloblastoma is a benign but locally aggressive odontogenic tumor. Worldwide, maxillary ameloblastoma is rare, but its late detection renders adequate treatment difficult. Majority occur in the mandible with about 5-20% occurring in the maxillary bone. Here we report a case of plexiform ameloblastoma of the left maxilla in a 30-year-old male. The tumor was presented as a radiographically solid mass filling the left maxillary sinus and clinically as a maxillary swelling. The radio-pathological features of this tumor and the possibility of its sinonasal epithelium origin are discussed.