bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure and organization of the human Theta-class glutathione S-transferase and d-dopachrome tautomerase gene complex
Authors: COGGAN Marjorie , WHITBREAD Lel , WHITTINGTON Angela , BOARD Philip 
Published: August 2015

The structure and organization of the human Theta-class glutathione S-transferase (GST) genes have been determined. GSTT1 and GSTT2 are separated by approx. 50 kb. They have a similar structure, being composed of five exons with identical exon/intron boundaries. GSTT1 is 8.1 kb in length, while GSTT2 is only 3.7 kb. The GSTT2 gene lies head-to-head with a gene encoding d-dopachrome tautomerase (DDCT), which extends over 8.5 kb and contains four exons. The sequence between GSTT2 and DDCT may contain a bidirectional promoter. The GSTT2 and DDCT genes have been duplicated in an inverted repeat. Sequence analysis of the duplicated GSTT2 gene has identified an exon 2/intron 2 splice site abnormality and a premature translation stop signal at codon 196. These changes suggest that the duplicate gene is a pseudogene, and it has been named GSTT2P.
Evidence that mammalian phosphatidylinositol transfer protein regulates phosphatidylcholine metabolism
Authors: MONACO Marie E. , ALEXANDER Richard J. , SNOEK Gerry T. , MOLDOVER Nancy H. , WIRTZ Karel W. A. , WALDEN Paul D. 
Published: August 2015

Phosphatidylinositol transfer proteins (PITPs) and their yeast counterpart (SEC14p) possess the ability to bind phosphatidylinositol (PtdIns) and transfer it between membranes in vitro. However, the biochemical function of these proteins in vivo is unclear. In the present study, the physiological role of PITP was investigated by determining the biochemical consequences of lowering the cellular content of this protein. WRK-1 rat mammary tumour cells were transfected with a plasmid containing a full-length rat PITPα cDNA inserted in the antisense orientation and the resultant cell clones were analysed. Three clones expressing antisense mRNA for PITPα were compared with three clones transfected with the expression vector lacking the insert. The three antisense clones had an average of 25% less PITPα protein than control clones. Two of the three antisense clones also exhibited a decreased rate of growth. All three antisense clones exhibited a significant decrease in the incorporation of labelled precursors into PtdCho during a 90-min incubation period. Under the same conditions, however, there was no change in precursor incorporation into PtdIns. Further experimentation indicated that the decrease in precursor incorporation seen in antisense clones was not due to an increased rate of turnover. When choline metabolism was analysed more extensively in one control (2-5) and one antisense (4-B) clone using equilibrium-labelling conditions (48 h of incubation), the following were observed: (1) the decrease in radioactive labelling of PtdCho seen in short-term experiments was also observed in long-term experiments, suggesting that the total amount of PtdCho was lower in antisense-transfected clones (this was confirmed by mass measurements); (2) a similar decrease was seen in cellular sphingomyelin, lysoPtdCho and glycerophosphorylcholine; (3) an average two-fold increase in cellular phosphorylcholine was observed in the antisense-transfected clone; (4) cellular choline was, on average, decreased; and (5) cellular CDPcholine was not significantly altered.
Human MUC5AC mucin dimerizes in the rough endoplasmic reticulum, similarly to the MUC2 mucin
Authors: ASKER Noomi , AXELSSON Magnus A. B. , OLOFSSON Sven-Olof , HANSSON Gunnar C. 
Published: August 2015

Biosynthetic studies on the human MUC5AC mucin were performed by immunoprecipitations with antisera recognizing only the non-O-glycosylated apomucin in the colon adenocarcinoma cell line LS 174T. Pulse–chase studies and subcellular fractionations showed that MUC5AC formed dimers in the rough endoplasmic reticulum within 15 min of the initiation of biosynthesis. No non-O-glycosylated species larger than dimers were identified. The dimerization was N-glycosylation-dependent, because tunicamycin treatment significantly lowered the rate of dimerization. When the biosynthesis of MUC5AC apomucin was compared with that of MUC2 apomucin, also produced in the LS 174T cell line, both apomucins were assembled in similar ways with respect to their rates of dimerization with and without inhibition of N-glycosylation. No heterodimerization was observed between the human MUC5AC and the MUC2 apomucins despite the extensive sequence similarities in the positions of the cysteine residues in the C-termini proposed to be involved in mucin dimerization.
Involvement of a local Fenton reaction in the reciprocal modulation by O2 of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase gene and the insulin-dependent activation of the glucokinase gene in rat hepatocytes
Authors: KIETZMANN Thomas , PORWOL Torsten , ZIEROLD Karl , JUNGERMANN Kurt , ACKER Helmut 
Published: August 2015

H2O2 mimicked the action of periportal pO2 in the modulation by O2 of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase (PCK) gene and the insulin-dependent activation of the glucokinase (GK) gene. H2O2 can be converted in the presence of Fe2+ in a Fenton reaction into hydroxyl anions and hydroxyl radicals (•OH). The hydroxyl radicals are highly reactive and might interfere locally with transcription factors. It was the aim of the present study to investigate the role of and to localize such a Fenton reaction. Hepatocytes cultured for 24 h were treated under conditions mimicking periportal or perivenous pO2 with glucagon or insulin plus the iron chelator desferrioxamine (DSF) or the hydroxyl radical scavenger dimethylthiourea (DMTU) to inhibit the Fenton reaction. PCK mRNA was induced by glucagon maximally under conditions of periportal pO2 and half-maximally under venous pO2. GK mRNA was induced by insulin with reciprocal modulation by O2. DSF and DMTU reduced the induction of PCK mRNA to about half-maximal and increased the induction of GK mRNA to maximal under both O2 tensions. Hydroxyl radical formation was maximal under arterial pO2. Perivenous pO2, DSF and DMTU each decreased the formation of •OH to about 70% of control. The Fenton reaction could be localized in a perinuclear space by confocal laser microscopy and three-dimensional reconstruction techniques. In the same compartment, iron could be detected by electron-probe X-ray microanalysis. Thus a local Fenton reaction is involved in the O2 signalling, which modulated the glucagon- and insulin-dependent PCK gene and GK gene activation.
Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases
Authors: LUNZER Regina , MAMNUN Yasmine , HALTRICH Dietmar , KULBE Klaus D. , NIDETZKY Bernd 
Published: August 2015

The NAD+-dependent xylitol dehydrogenase from the xylose-assimilating yeast Galactocandida mastotermitis has been purified in high yield (80%) and characterized. Xylitol dehydrogenase is a heteronuclear multimetal protein that forms homotetramers and contains 1 mol of Zn2+ ions and 6 mol of Mg2+ ions per mol of 37.4 kDa protomer. Treatment with chelating agents such as EDTA results in the removal of the Zn2+ ions with a concomitant loss of enzyme activity. The Mg2+ ions are not essential for activity and are removed by chelation or extensive dialysis without affecting the stability of the enzyme. Results of initial velocity studies at steady state for d-sorbitol oxidation and d-fructose reduction together with the characteristic patterns of product inhibition point to a compulsorily ordered Theorell–Chance mechanism of xylitol dehydrogenase in which coenzyme binds first and leaves last. At pH 7.5, the binding of NADH (Ki ≈ 10 µM) is approx. 80-fold tighter than that of NAD+. Polyhydroxyalcohols require at least five carbon atoms to be good substrates of xylitol dehydrogenase, and the C-2 (S), C-3 (R) and C-4 (R) configuration is preferred. Therefore xylitol dehydrogenase shares structural and functional properties with medium-chain sorbitol dehydrogenases.
Design of a transferrin–proteinase inhibitor conjugate to probe for active cysteine proteinases in endosomes
Authors: XING Ruye , MASON Robert W. 
Published: August 2015

A new technique has been developed to identify active proteinases in endosomes that does not require prior isolation of organelles and extraction of the active enzymes. [125I]Iodotyrosylalanyldiazomethane was reversibly conjugated to transferrin to selectively deliver it to endosomes. The protein was conjugated to the inhibitor via a disulphide bond using N-succinimidyl 3-(2-pyridyldithio)propionate. The inhibitor portion of the conjugate bound irreversibly to active cathepsins B and L, and subsequently the reacted enzymes were separated from the transferrin after SDS/PAGE under reducing conditions. Uptake of the protein–inhibitor conjugate and incorporation of inhibitor into cathepsins was blocked at 4 °C, demonstrating that the conjugate enters cells by receptor-mediated endocytosis. Furthermore, endocytosed transferrin–inhibitor conjugate could be recycled back to the extracellular medium and binding to the transferrin receptor could be blocked by native transferrin. Labelling of the enzymes was not blocked by incubating cells at 16 °C, consistent with the majority of the reagent being targeted to endosomes. The inhibited enzymes remained conjugated to transferrin, showing that the disulphide bond between the transferrin and inhibitor was not reduced in the endosome. Results from these studies show that endosomes contain both intermediate and late biosynthetic forms of active cathepsin B, which are indistinguishable from those found in mature lysosomes. These results indicate that the active enzymes in endosomes are not early biosynthetic forms in transit to lysosomes but most probably enter the endosome via retrograde traffic from the lysosome.
The Impact of Preoperative Depression and Health State on Quality-of-Life Outcomes after Anterior Cervical Diskectomy and Fusion
Authors: Alvin Matthew D. , Miller Jacob A. , Lubelski Daniel , Nowacki Amy S. , Scheman Judith , Mathews Manu , McGirt Matthew J. , Benzel Edward C. , Mroz Thomas E. 
Published: August 2015

Study Design Retrospective cohort study. Objective We sought to assess the predictive value of preoperative depression and health state on 1-year quality-of-life outcomes after anterior cervical diskectomy and fusion (ACDF). Methods We analyzed 106 patients who underwent ACDF. All patients had either bilateral or unilateral cervical radiculopathy. Preoperative and 1-year postoperative health outcomes were assessed based on the visual analog scale, Pain Disability Questionnaire (PDQ), Patient Health Questionnaire (PHQ-9), and EuroQol-5 Dimensions (EQ-5D) questionnaire. Univariable and multivariate regression analyses were performed to assess for preoperative predictors of 1-year change in health status according to the EQ-5D. Results Compared with preoperative health states, the ACDF cohort showed statistically significant improved PDQ (78.5 versus 57.9), PHQ-9 (9.7 versus 5.3), and EQ-5D (0.55 versus 0.68) scores at 1 year postoperatively and surpassed the minimum clinically important difference for the EQ-5D of 0.1 units (all p ≤ 0.01). Multivariate linear regression indicated that anxiolytic use and higher EQ-5D preoperative scores were associated with less 1-year postoperative improvement in health status. Although not statistically significant, clinically important effects of preoperative depression, as measured by the PHQ-9, were observed on postoperative QOL outcome (−0.006, 95% confidence interval −0.014 to 0.001). Conclusions Of patients who undergo ACDF with similar preoperative QOL health states, those with a greater degree of depression may have lower improvements in postoperative QOL compared with those with less depression. Patients with anxiety and better preoperative health states also attain less 1-year QOL improvements.
The Appointment of Judges by Ministers
Authors: Hanretty Chris 
Published: August 2015

AbstractI investigate appointment to the Court of Appeal and House of Lords between 1880 and 2005. Exploiting the fact that appointment is almost invariably from within the ranks of existing High Court judges and using a conditional logit model, I test for effects of legal, professional, and political factors on appointment prospects. Although there is no advantage to having the same political affiliation as the appointing lord chancellor, judges are more likely to be promoted if they were previously appointed by the incumbent party.
Student feedback: a learning and teaching performance indicator
Authors: Kinash Shelley , Naidu Vishen , Knight Diana , Judd Madelaine-Marie , Nair Chenicheri Sid , Booth Sara , Fleming Julie , Santhanam Elizabeth , Tucker Beatrice , Tulloch Marian 
Published: August 2015

Purpose
– The paper aims to disseminate solutions to common problems in student evaluation processes. It proposes that student evaluation can be applied to quality assurance and improving learning and teaching. The paper presents solutions in the areas of: presenting outcomes as performance indicators, constructing appropriate surveys, improving response rates, reporting student feedback to students and student engagement as a feature of university quality assurance.

Design/methodology/approach
– The research approach of this paper is comparative case study, allowing in-depth exploration of multiple perspectives and practices at seven Australian universities. Process and outcome data were rigorously collected, analysed, compared and contrasted.

Findings
– The paper provides empirical evidence for student evaluation as an instrument of learning and teaching data analysis for quality improvement. It suggests that collecting data about student engagement and the student experience will yield more useful data about student learning. Furthermore, findings indicate that students benefit from more authentic inclusion in the evaluation process and outcomes.

Research limitations/implications
– Because of the chosen research approach, the research results may lack generalisability. Therefore, researchers are encouraged to test the proposed propositions further and apply to their own university contexts.

Practical implications
– The paper includes recommendations at the institution- and sector-wide levels to effectively use student evaluation as a university performance indicator and as a tool of change.

Originality/value
– This paper fulfils an identified need to examine student evaluation processes across institutions and focuses on the role of student evaluation in quality assurance.
Social participation experiences of mothers of children with cerebral palsy in an Iranian context
Authors: Dehghan Leila , Dalvandi Asghar , Rassafiani Mehdi , Hosseini Seyed Ali , Dalvand Hamid , Baptiste Sue 
Published: August 2015

Background/aimSocial participation is increasingly of interest in research that investigates the impact of caring for a child with a disability. Little has been investigated about the social participation experiences of mothers of children with cerebral palsy (CP). This study explored social participation among Iranian mothers of children with CP.MethodsThe conventional qualitative content analysis method was utilised. Data were collected via in‐depth semi‐structured interviews with 14 mothers (aged 26–45 years) of children with CP with the gross motor function classification system expanded & revised levels III–V. Constant comparative analysis was deployed for data analysis.ResultsThe results were identified and classified into three main themes: (i) polarisation of positive and negative feelings; (ii) challenges to mothers’ social activity; and (iii) striving to engage in society.ConclusionIn the Iranian context, mothers of children with CP are facing many challenges to social participation and seem to have been neglected by the health‐care system. One of the priorities of Iranian health policy makers may be developing, establishing and implementing social support to enable the mothers to participate in social activities. Furthermore, occupational therapists can contribute and guide mothers’ social participation by creating programs to develop and utilise skills for them.
The Homicide–Suicide Phenomenon: Findings of Psychological Autopsies
Authors: Knoll James L. , Hatters‐Friedman Susan 
Published: August 2015

AbstractHomicide–suicide represents a single episode of violence which may decimate an entire family. This study aimed to further describe motives and context of these tragedies. Psychological autopsies were completed for 18 homicide–suicide cases in Dallas, Texas. This included postevent interviews with surviving family members and review of police and coroner records. Two‐thirds of perpetrators had made either verbal or written threats prior to the homicide–suicide. A simplified typology describing victim–perpetrator relationship and motive type is suggested for future studies and clinical ease. Two‐thirds of perpetrators fell into the category of Intimate‐Possessive, most of whom were depressed men who were abusing substances and undergoing separation. Additional categories included Intimate‐Ailing, Filial‐Revenge, Familial‐Psychotic, and Friend‐Psychotic. Further, implications from this psychological autopsy study regarding risk assessment include use of collateral interviews regarding threats and past violence.
A Comprehensive and Harmonized Digital Forensic Investigation Process Model
Authors: Valjarevic Aleksandar , Venter Hein S. 
Published: August 2015

AbstractPerforming a digital forensic investigation (DFI) requires a standardized and formalized process. There is currently neither an international standard nor does a global, harmonized DFI process (DFIP) exist. The authors studied existing state‐of‐the‐art DFIP models and concluded that there are significant disparities pertaining to the number of processes, the scope, the hierarchical levels, and concepts applied. This paper proposes a comprehensive model that harmonizes existing models. An effort was made to incorporate all types of processes proposed by the existing models, including those aimed at achieving digital forensic readiness. The authors introduce a novel class of processes called concurrent processes. This is a novel contribution that should, together with the rest of the model, enable more efficient and effective DFI, while ensuring admissibility of digital evidence. Ultimately, the proposed model is intended to be used for different types of DFI and should lead to standardization.
Suspect Height Estimation Using the Faro Focus3D Laser Scanner
Authors: Johnson Monique , Liscio Eugene 
Published: August 2015

AbstractAt present, very little research has been devoted to investigating the ability of laser scanning technology to accurately measure height from surveillance video. The goal of this study was to test the accuracy of one particular laser scanner to estimate suspect height from video footage. The known heights of 10 individuals were measured using an anthropometer. The individuals were then recorded on video walking along a predetermined path in a simulated crime scene environment both with and without headwear. The difference between the known heights and the estimated heights obtained from the laser scanner software were compared using a one‐way t‐test. The height estimates obtained from the software were not significantly different from the known heights whether individuals were wearing headwear (p = 0.186) or not (p = 0.707). Thus, laser scanning is one technique that could potentially be used by investigators to determine suspect height from video footage.
The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces
Authors: Tsai Li‐Chin , Lee Cheng‐Chang , Chen Chun‐Chieh , Lee James Chun‐I , Wang Sheng‐Meng , Huang Nu‐En , Linacre Adrian , Hsieh Hsing‐Mei 
Published: August 2015

AbstractFingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real‐time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2‐indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2‐indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2‐indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces.
Most Challenging of Contexts
Authors: Corsaro Nicholas , Engel Robin S. 
Published: August 2015

Research SummaryThe use of focused deterrence to reduce lethal violence driven by gangs and groups of chronic offenders has continued to expand since the initial Boston Ceasefire intervention in the 1990s, where prior evaluations have shown relatively consistent promise in terms of violence reduction. This study focuses on the capacity of focused deterrence to impact lethal violence in a chronic and high‐trajectory homicide setting: New Orleans, Louisiana. Using a two‐phase analytical design, our evaluation of the Group Violence Reduction Strategy (GVRS) observed the following findings: (a) GVRS team members in the City of New Orleans closely followed model implementation; (b) homicides in New Orleans experienced a statistically significant reduction above and beyond changes observed in comparable lethally violent cities; (c) the greatest changes in targeted outcomes were observed in gang homicides, young Black male homicides, and firearms violence; and (d) the decline in targeted violence corresponded with the implementation of the pulling levers notification meetings. Moreover, the observed reduction in crime outcomes was not empirically associated with a complementary violence‐reduction strategy that was simultaneously implemented in a small geographic area within the city.Policy ImplicationsThe findings presented in this article demonstrate that focused deterrence holds considerable promise as a violence prevention approach in urban contexts with persistent histories of lethal violence, heightened disadvantage, and undermined police (and institutional) legitimacy. The development of a multiagency task force, combined with unwavering political support from the highest levels of government within the city, were likely linked to high programmatic fidelity. Organizationally, the development of a program manager and intelligence analyst, along with the use of detailed problem analyses and the integration of research, assisted the New Orleans working group in identifying the highest risk groups of violent offenders to target for the GVRS notification sessions. The impacts on targeted violence were robust and consistent with the timing of the intervention.