bluuebunny/crossref_metadata_2025_split huggingface dataset:
NF-κB2/p52:c-Myc:hnRNPA1 Pathway Regulates Expression of Androgen Receptor Splice Variants and Enzalutamide Sensitivity in Prostate Cancer
Authors: Nadiminty Nagalakshmi , Tummala Ramakumar , Liu Chengfei , Lou Wei , Evans Christopher P. , Gao Allen C. 
Published: June 2015

Abstract
Castration-resistant prostate cancer (CRPC) remains dependent on androgen receptor (AR) signaling. Alternative splicing of the AR to generate constitutively active, ligand-independent variants is one of the principal mechanisms that promote the development of resistance to next-generation antiandrogens such as enzalutamide. Here, we demonstrate that the splicing factor heterogeneous nuclear RNA-binding protein A1 (hnRNPA1) plays a pivotal role in the generation of AR splice variants such as AR-V7. hnRNPA1 is overexpressed in prostate tumors compared with benign prostates, and its expression is regulated by NF-κB2/p52 and c-Myc. CRPC cells resistant to enzalutamide exhibit higher levels of NF-κB2/p52, c-Myc, hnRNPA1, and AR-V7. Levels of hnRNPA1 and AR-V7 are positively correlated with each other in prostate cancer. The regulatory circuit involving NF-κB2/p52, c-Myc, and hnRNPA1 plays a central role in the generation of AR splice variants. Downregulation of hnRNPA1 and consequently of AR-V7 resensitizes enzalutamide-resistant cells to enzalutamide, indicating that enhanced expression of hnRNPA1 may confer resistance to AR-targeted therapies by promoting the generation of splice variants. These findings may provide a rationale for cotargeting these pathways to achieve better efficacy through AR blockade. Mol Cancer Ther; 14(8); 1884–95. ©2015 AACR.
Endo-Epicardial Versus Only-Endocardial Ablation as a First Line Strategy for the Treatment of Ventricular Tachycardia in Patients With Ischemic Heart Disease
Authors: Izquierdo Maite , Sánchez-Gómez Juan Miguel , Ferrero de Loma-Osorio Angel , Martínez Angel , Bellver Alejandro , Peláez Antonio , Núñez Julio , Núñez Carlos , Chorro Javier , Ruiz-Granell Ricardo 
Published: June 2015

Background—
Epicardial ablation has shown improvement in clinical outcomes of patients with ischemic heart disease (IHD) after ventricular tachycardia (VT) ablation. However, usually epicardial access is only performed when endocardial ablation has failed. Our aim was to compare the efficacy of endocardial+epicardial ablation versus only endocardial ablation in the first procedure in patients with IHD.

Methods and Results—

Fifty-three patients with IHD, referred for a first VT ablation to our institution, from 2012 to 2014, were included. They were divided in 2 groups according to enrollment time: from May 2013, we started to systematically perform endo-epicardial access (Epi-Group) as first-line approach in consecutive patients with IHD (n=15). Patients who underwent only an endocardial VT ablation in their first procedure (Endo-Group) included patients with previous cardiac surgery and the historical (before May 2013; n=35). All late-potentials in the scar zone were eliminated, and if VT was tolerated, critical isthmuses were also approached. The end point was the noninducibility of any VT. During a median follow-up of 15±10 months, the combined end point (hospital or emergency admission because of a ventricular tachycardia or reablation) occurred in 14 patients of the Endo-group and in one patient in the Epi-group (event-free survival curves by Grey-test,
P
=0.03). Ventricular arrhythmia recurrences occurred in 16 and in 3 patients in the Endo and Epi-Group, respectively (Grey-test,
P
=0.2).

Conclusions—
A combined endocardial–epicardial ablation approach for initial VT ablation was associated with fewer readmissions for VT and repeat ablations. Further studies are warranted.
Rapid and sensitive analysis of reduced and oxidized coenzyme Q10 in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and application to studies in healthy human subjects
Authors: Claessens Adam J , Yeung Catherine K , Risler Linda J , Phillips Brian R , Himmelfarb Jonathan , Shen Danny D 
Published: June 2015

Background Coenzyme Q10 is an endogenous antioxidant as well as a popular dietary supplement. In blood circulation, coenzyme Q10 exists predominantly as its reduced ubiquinol-10 form, which readily oxidizes to ubiquinone-10 ex vivo. Plasma concentrations of coenzyme Q10 reflect net overall metabolic demand, and the ratio of ubiquinol-10:ubiquinone-10 has been established as an important biomarker for oxidative stress. However, the lability of ubiquinol-10 makes accurate determination of both forms of coenzyme Q10 difficult. Ex vivo oxidation of ubiquinol-10 to ubiquinone-10 during sample collection, processing and analysis may obfuscate the in vivo ratio. Methods We developed a rapid and sensitive method for the determination of ubiquinol-10 and ubiquinone-10 in human plasma, using coenzyme Q9 analogues as internal standards. Single-step protein precipitation in 1-propanol, a lipophilic and water-soluble alcohol, allowed for rapid extraction. Results Analysis by ultra performance liquid chromatography-tandem mass spectrometry provided rapid run-time and high sensitivity, with lower limits of quantitation for ubiquinol-10 and ubiquinone-10 of 5 μg/L and 10 μg/L, respectively. Conclusions This method is suitable for clinical studies with coenzyme Q10 supplementation in various disease states where this lipid-antioxidant may be beneficial. We have applied this method to >300 plasma samples from coenzyme Q10 research studies in chronic haemodialysis patients and postsurgical patients.
Relationship-focused parenting intervention to support developmental outcomes for a young child with cerebral palsy: A practice application
Authors: Barfoot Jacqui , Meredith Pamela , Ziviani Jenny , Whittingham Koa 
Published: June 2015

Statement of context The effectiveness of early intervention for children with developmental disabilities is thought to be related to parental sensitivity and responsiveness. A brief relationship-focused occupational therapy intervention for Shelly, a young girl with cerebral palsy, and her mother, Jane, is described. Critical reflection on practice The distress of both mother and child at presentation compromised the ability of therapists to provide developmentally orientated interventions. Focusing on parent–child interactions enabled Shelly to make both emotional and physical gains, and facilitated ongoing interventions. Implications for practice Observations in this practice application demonstrate the need for further research into parenting interventions and developmental outcomes for children with cerebral palsy.
Clinical Outcomes of Modified Mason-Allen Single-Row Repair for Bursal-Sided Partial-Thickness Rotator Cuff Tears
Authors: Shin Sang-Jin , Kook Seung-Hwan , Rao Nandan , Seo Myeong-Jae 
Published: June 2015

Background: Various repair techniques have been reported for the operative treatment of bursal-sided partial-thickness rotator cuff tears. Recently, arthroscopic single-row repair using a modified Mason-Allen technique has been introduced. Hypothesis: The arthroscopic, modified Mason-Allen single-row technique with preservation of the articular-sided tendon provides satisfactory clinical outcomes and similar results to the double-row suture-bridge technique after conversion of a partial-thickness tear to a full-thickness tear. Study Design: Cohort study; Level of evidence, 3. Methods: A retrospective study was conducted on 84 consecutive patients with symptomatic, bursal-sided partial-thickness rotator cuff tears involving more than 50% thickness of the tendon. A total of 47 patients were treated by the modified Mason-Allen single-row repair technique, preserving the articular-sided tendon, and 37 patients were treated by the double-row suture-bridge repair technique after conversion to a full-thickness tear. The clinical and functional outcomes were evaluated using the American Shoulder and Elbow Surgeons (ASES) and Constant scores and a visual analog scale (VAS) for pain and satisfaction of patients. Magnetic resonance imaging (MRI) was used to analyze the integrity of tendons at 6-month follow-up. Patients were followed up for a mean of 32.5 months. Results: In the 47 patients treated with the modified Mason-Allen suture technique, the VAS score decreased from a preoperative mean of 5.3 ± 0.3 to 0.9 ± 0.5 at the time of final follow-up. There was a statistically significant increase in the mean ASES score (from 45.4 ± 2.9 to 88.6 ± 4.5) and mean Constant score (from 66.9 ± 2.6 to 88.1 ± 2.4) ( P < .001). Four of 47 patients (8.5%) demonstrated retears at 6-month postoperative MRI. There was no statistical difference in terms of functional outcomes and the retear rate compared with those of patients with the suture-bridge repair technique (3 patients, 8.1%). However, the mean number of suture anchors used in the patients with modified Mason-Allen suture repair (1.2 ± 0.4) was significantly fewer than that in the patients with suture-bridge repair (3.2 ± 0.4) ( P < .01). Conclusion: The modified Mason-Allen single-row repair technique that preserved the articular-sided tendon provided satisfactory clinical outcomes in patients with symptomatic, bursal-sided partial-thickness rotator cuff tears. Despite a fewer number of suture anchors, the shoulder functional outcomes and retear rate in patients after modified Mason-Allen repair were comparable with those of patients who underwent double-row suture-bridge repair. Therefore, the modified Mason-Allen single-row repair technique using a triple-loaded suture anchor can be considered as an effective treatment in patients with bursal-sided partial-thickness rotator cuff tears.
Clinical Outcomes and Complications of Percutaneous Achilles Repair System Versus Open Technique for Acute Achilles Tendon Ruptures
Authors: Hsu Andrew R. , Jones Carroll P. , Cohen Bruce E. , Davis W. Hodges , Ellington J. Kent , Anderson Robert B. 
Published: June 2015

Background: Limited incision techniques for acute Achilles tendon ruptures have been developed in recent years to improve recovery and reduce postoperative complications compared with traditional open repair. The purpose of this retrospective cohort study was to analyze the clinical outcomes and postoperative complications between acute Achilles tendon ruptures treated using a percutaneous Achilles repair system (PARS [Arthrex, Inc, Naples, FL]) versus open repair and evaluate the overall outcomes for operatively treated Achilles ruptures. Methods: Between 2005 and 2014, 270 consecutive cases of operatively treated acute Achilles tendon ruptures were reviewed (101 PARS, 169 open). Patients with Achilles tendinopathy, insertional ruptures, chronic tears, or less than 3-month follow-up were excluded. Operative treatment consisted of a percutaneous technique (PARS) using a 2-cm transverse incision with FiberWire (Arthrex, Inc, Naples, FL) sutures or open repair using a 5- to 8-cm posteromedial incision with FiberWire in a Krackow fashion reinforced with absorbable sutures. Patient demographics were recorded along with medical comorbidities, activity at injury, time from injury to surgery, length of follow-up, return to baseline activities by 5 months, and postoperative complications. Results: The most common activity during injury for both groups was basketball (PARS: 39%, open: 47%). A greater number of patients treated with PARS were able to return to baseline physical activities by 5 months compared with the open group (PARS: 98%, open: 82%; P = .0001). There were no significant differences ( P > .05) between groups in rates of rerupture ( P = 1.0), sural neuritis ( P = .16), wound dehiscence ( P = .74), superficial ( P = .29) and/or deep infection ( P = .29), or reoperation ( P = .13). There were no deep vein thromboses (DVTs) or reruptures in either group. In the PARS group, there were no cases of sural neuritis, 3 cases (3%) of superficial wound dehiscence, and 2 reoperations (2%) for superficial foreign-body reaction to FiberWire. In the open group, there were 5 cases (3%) of sural neuritis, 7 cases (4%) of superficial wound dehiscence, 3 cases (2%) of superficial infection, and 3 reoperations (2%) for deep infection. Conclusion: The present study reports the largest single-center series of acute Achilles tendon ruptures in the literature with lower complication rates for operatively treated Achilles ruptures compared with previous reports. The overall complication rate for all operatively treated Achilles ruptures was 8.5% with no reruptures, and most patients (88%) were able to return to baseline activities by 5 months after surgery. There were no significant differences in rates of postoperative complications between PARS and open repair for acute Achilles tendon ruptures. Level of Evidence: Level III, retrospective cohort study.
Systematic review and meta-analysis of migration after endovascular abdominal aortic aneurysm repair
Authors: Spanos Konstantinos , Karathanos Christos , Saleptsis Vasileios , Giannoukas Athanasios D 
Published: June 2015

Aim To identify patients who are under higher threat for migration because of an old generation stent graft application. Methods A systematic review and meta-analysis of the literature was undertaken to identify all studies which included older generation endografts and data reporting on graft migration after EVAR. Outcome data were pooled and combined, and were calculated using fixed or random effects models. Results From 2000 to 2014, 22 retrospective studies were identified reporting on stent- graft migration after EVAR (8.6%). From those patients, 39% received re-intervention with the mean time of identification ranging from 12 to 36 months. Six of these retrospective nonrandomized studies were eligible for meta-analysis. AAA diameter (AAA diameter: 0.719 mm; 95% confidence interval [CI]: 0.00065–1.4384 mm; p = 0.00497) and neck length (neck length: 4.36 mm; 95% CI: 1.3277–7.394; p = 0.0048) were the only significant factors associated with stent- graft migration. Neck diameter and neck angulation did not have any important influence on stent-graft migration. Conclusions Patients with large AAA and short necks who were treated with older generation stent grafts such as AneurX and Talent are in higher risk for endograft migration than others. Stent- graft migration consists of an insidious and underestimated threat.
Art, fisheries and ethnobiology
Authors: Begossi Alpina , Caires Rodrigo 
Published: June 2015

AbstractBackgroundNature is perceived in a variety of forms, and the perception of nature can also be expressed in different ways. Local art may represent the perception of nature by humans. It can embody perception, imagination and wisdom. Local art, in particular, reflects how people interact with nature. For example, when studying the representation of fish by different cultures, it is possible to access information on the fish species found in the environment, on its relative importance, and on historical events, among others. In this context, art can be used to obtain information on historical events, species abundance, ecology, and behaviour, for example. It can also serve to compare baselines by examining temporal and spatial scales. This study aims to analyse art and nature from a human ecological perspective: art can understood as an indicator of fish abundance or salience.Art has a variety of dimensions and perspectives. Art can also be associated with conservation ecology, being useful to reinterpret ecological baselines. A variety of paintings on fish, as well as paintings from local art, are explored in this study. They are analyzed as representing important fish, spatially and historically.MethodsA survey regarding the fish found in different paintings was conducted using art books and museum books. Pictures were taken by visiting museums, particularly for local or traditional art (Australia and Cape Town).ResultsThe fish illustrated here seem to be commonly important in terms of salience. For example,Coryphaenaspp. is abundant in Greece, Nile tilapia in Egypt,Gadus morhuain the Netherlands, as well as barracuda in Australia; salience is also applied to useful, noticeable or beautiful organisms, such asCarassius auratus(China). Another aspect of salience, the diversity of a group, is also represented by the panel whereUraspis uraspisappears to be depicted.ConclusionsRegarding the evaluation of baselines, we should consider that art may represent abundant fish in certain historic periods and geographic regions. Art could be an important temporal and geographical indicator to discover preterit information on the abundance of fish and compare it to present abundance.
Matching Behavior as a Tradeoff Between Reward Maximization and Demands on Neural Computation
Authors: Kubanek Jan , Snyder Lawrence H. 
Published: June 2015

When faced with a choice, humans and animals commonly distribute their behavior in proportion to the frequency of payoff of each option. Such behavior is referred to as matching and has been captured by the matching law. However, matching is not a general law of economic choice. Matching in its strict sense seems to be specifically observed in tasks whose properties make matching an optimal or a near-optimal strategy. We engaged monkeys in a foraging task in which matching was not the optimal strategy. Over-matching the proportions of the mean offered reward magnitudes that would yield more reward than matching, yet, surprisingly, the animals almost exactly matched them. To gain insight into this phenomenon, we modeled the animals' decision-making using a mechanistic model. The model accounted for the animals' macroscopic and microscopic choice behavior. When the models' three parameters were not constrained to mimic the monkeys' behavior, the model over-matched the reward proportions and in doing so, harvested substantially more reward than the monkeys. This optimized model revealed a marked bottleneck in the monkeys' choice function that compares the value of the two options. The model featured a very steep value comparison function relative to that of the monkeys. The steepness of the value comparison function had a profound effect on the earned reward and on the level of matching. We implemented this value comparison function through responses of simulated biological neurons. We found that due to the presence of neural noise, steepening the value comparison requires an exponential increase in the number of value-coding neurons. Matching may be a compromise between harvesting satisfactory reward and the high demands placed by neural noise on optimal neural computation.
Getting information from the cloud storage in the investigation of incidents in the field of information security
Authors: Нестеров Антон , Nesterov Anton , Баркалов Юрий , Barkalov Yuriy 
Published: June 2015

Relevance of the work is due to the increase of crimes committed with the use of cloud technologies and lack of scientific support and research methods of obtaining information located in the cloud storage.
Currently, there is a significant increase in incidents committed using cloud technologies. Therefore, to obtain forensically important information from the cloud storage is an urgent task. The process of obtaining such information is a complex technical actions that require a lot of experience and time. The data obtained in this work, experts will optimize actions to improve the efficiency analysis of incidents in the area of ​​information security related to the use of cloud technologies, both through optimization of labor and time costs, and due to the quality of research results.
Tumor MicroRNA Expression Profiling Identifies Circulating MicroRNAs for Early Breast Cancer Detection
Authors: Matamala Nerea , Vargas María Teresa , González-Cámpora Ricardo , Miñambres Rebeca , Arias José Ignacio , Menéndez Primitiva , Andrés-León Eduardo , Gómez-López Gonzalo , Yanowsky Kira , Calvete-Candenas Julio , Inglada-Pérez Lucía , Martínez-Delgado Beatriz , Benítez Javier 
Published: June 2015

Abstract

BACKGROUND
The identification of novel biomarkers for early breast cancer detection would be a great advance. Because of their role in tumorigenesis and stability in body fluids, microRNAs (miRNAs) are emerging as a promising diagnostic tool. Our aim was to identify miRNAs deregulated in breast tumors and evaluate the potential of circulating miRNAs in breast cancer detection.

METHODS
We conducted miRNA expression profiling of 1919 human miRNAs in paraffin-embedded tissue from 122 breast tumors and 11 healthy breast tissue samples. Differential expression analysis was performed, and a microarray classifier was generated. The most relevant miRNAs were analyzed in plasma from 26 healthy individuals and 83 patients with breast cancer (36 before and 47 after treatment) and validated in 116 healthy individuals and 114 patients before treatment.

RESULTS
We identified a large number of miRNAs deregulated in breast cancer and generated a 25-miRNA microarray classifier that discriminated breast tumors with high diagnostic sensitivity and specificity. Ten miRNAs were selected for further investigation, of which 4 (miR-505-5p, miR-125b-5p, miR-21-5p, and miR-96-5p) were significantly overexpressed in pretreated patients with breast cancer compared with healthy individuals in 2 different series of plasma. MiR-505-5p and miR-96-5p were the most valuable biomarkers (area under the curve 0.72). Moreover, the expression levels of miR-3656, miR-505-5p, and miR-21-5p were decreased in a group of treated patients.

CONCLUSIONS
Circulating miRNAs reflect the presence of breast tumors. The identification of deregulated miRNAs in plasma of patients with breast cancer supports the use of circulating miRNAs as a method for early breast cancer detection.
Strain and Pressure Gauges from Tough, Conducting and Edible Hydrogels
Authors: Keller Alex , Benz Dominik , in het Panhuis Marc 
Published: June 2015

ABSTRACTThe development of highly conductive, robust edible hydrogels was investigated using a combination of the biopolymers gellan gum and gelatin, a common salt (NaCl) and plant-derived cross-linker (genipin). Robust strain gauge/pressure sensors were developed using edible materials to demonstrate the potential of these hydrogels. The hydrogels exhibited gauge factor and pressure sensitivity values of 7.6 ± 0.1 and 400 ± 7 μΩ/Pa for loads up to 3 kPa, respectively. Furthermore, these devices were able to operate under larger loads with gauge factor and pressure sensitivity values of 0.308 ± 0.002 and 7.17 ± 0.05 μΩ/Pa, respectively, for loads between 9 kPa and 280 kPa.
Analysis of LiFePO4 Cathodic Active Material Synthesized in Open Environment Conditions through Ionic Medium
Authors: Kwee Darren W. , Lim Taehoon , Martinez-Morales Alfredo 
Published: June 2015

ABSTRACTIn this work, LiFePO4(LFP) particles were synthesized through an ionic liquid medium. Through the fabrication of LFP particles, we observed the formation of quasi-1−dimensional (1D) structures. The characterization of phases found in the reaction, through time-dependent studies, have led us to propose a possible scheme for particle formation.Synthesized material was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and x-ray diffraction (XRD). We also report our analysis on particle morphology and crystallinity of LFP particles synthesized through an ionic liquid−mediated process.