bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of microencapsulated plant essential oils on growth performance, immunity, and intestinal health of weaned Tibetan piglets
Authors: Chen Xiaolian , Song Wenjing , Xiong Pingwen , Cheng Di , Wei Weiqun , Zhou Quanyong , Xu Chuanhui , Song Qiongli , Ji Huayuan , Hu Yan , Zou Zhiheng 
Published: August 2024

IntroductionPlant essential oils (PEOs) have received significant attention in animal production due to their diverse beneficial properties and hold potential to alleviate weaning stress. However, PEOs effectiveness is often compromised by volatility and degradation. Microencapsulation can enhance the stability and control release rate of essential oils. Whether different microencapsulation techniques affect the effectiveness remain unknown. This study aimed to investigate the effects of PEOs coated by different microencapsulation techniques on growth performance, immunity, and intestinal health of weaned Tibetan piglets.MethodsA total of 120 Tibetan piglets, aged 30 days, were randomly divided into five groups with four replicates, each containing six piglets. The experimental period lasted for 32 days. The groups were fed different diets: a basal diet without antibiotics (NC), a basal diet supplemented with 10 mg/kg tylosin and 50 mg/kg colistin sulfate (PC), 300 mg/kg solidified PEO particles (SPEO), 300 mg/kg cold spray-coated PEO (CSPEO), or 300 mg/kg hot spray-coated PEO (HSPEO).ResultsThe results showed that supplementation with SPEO, CSPEO, or HSPEO led to a notable decrease in diarrhea incidence and feed to gain ratio, as well as duodenum lipopolysaccharide content, while simultaneously increase in average daily gain, interleukin-10 (IL-10) levels and the abundance of ileum Bifidobacterium compared with the NC group (p &lt; 0.05). Supplementation with SPEO, CSPEO, or HSPEO significantly elevated serum immunoglobulin G (IgG) levels and concurrently reduced serum lipopolysaccharide and interferon γ levels compared with the NC and PC groups (p &lt; 0.05). Serum insulin-like growth factor 1 (IGF-1) levels in the SPEO and HSPEO groups significantly increased compared with the NC group (p &lt; 0.05). Additionally, CSPEO and HSPEO significantly reduced jejunum pH value (p &lt; 0.05) compared with the NC and PC groups (p&lt;0.05). Additionally, Supplementation with HSPEO significantly elevated levels of serum immunoglobulin M (IgM) and interleukin-4 (IL-4), abundance of ileum Lactobacillus, along with decreased serum interleukin-1 beta (IL-1β) levels compared with both the NC and PC groups.DiscussionOur findings suggest that different microencapsulation techniques affect the effectiveness. Dietary supplemented with PEOs, especially HSPEO, increased growth performance, improved immune function, and optimized gut microbiota composition of weaned piglets, making it a promising feed additive in piglet production.
A New Species of Cyrtodactylus tibetanus Group (Reptilia: Squamata: Gekkonidae) from Xizang Autonomous Region, China
Authors: Ma Shun , Shi Sheng-Chao , Qian Tian-Yu , Sui Lu-Lu , Wang Bin , Jiang Jian-Ping 
Published: August 2024

A new Cyrtodactylus species, C. laevissp. nov., from the dry-hot valleys near the Yarlung Zangbo River in Re Village, Jindong Countryside, Lang County, Linzhi City, Xizang Autonomous Region, China, is described herein based upon the integrative taxonomic results combining molecular phylogenetic systematics and morphological characteristic comparisons. Our molecular phylogeny was inferred by combining three mitochondrial gene fragments (16S/CO1/ND2), and it indicated a distinct differentiation between the new species and C. tibetanus species complex, with obvious genetic distances (16S 9.9–11.8%/CO1 16.5–18.2%/ND2 16.6–18.5%) detected, supporting its validity. Morphologically, the new species can be easily distinguished from its congers by the following characters: (1) medium size (SVL 48.58–50.92 mm), (2) tubercles on dorsum sparse, (3) tail segments absent and tubercles on tails absent, (4) supralabials 10–12 and infralabials 8–10, (5) interorbital scales between anterior corners of the eyes 28–32, (6) scale rows at midbody 96–98, (7) ventral scales between mental and cloacal slit 145–153, (8) ventral scale rows 41–45, and (9) 4 to 5 white–yellow transverse bands with brown dots and black merges between the nape and sacrum. The description of C. laevis sp. nov. increased the total species number of C. tibetanus group to three, and the total Cyrtodactylus species number in Xizang to six and in China to eleven. The new species is currently only known from the type locality with its extremely small populations and needs future surveys to reveal its distribution range, population status, natural history, and mechanisms so that the new species can coexist with Altiphylax medogense.
Analysis of Structural Changes of pH–Thermo-Responsive Nanoparticles in Polymeric Hydrogels
Authors: Ruiz-Virgen Lazaro , Hernandez-Martinez Miguel Angel , Martínez-Mejía Gabriela , Caro-Briones Rubén , Herbert-Pucheta Enrique , Río José Manuel del , Corea Mónica 
Published: August 2024

The pH- and thermo-responsive behavior of polymeric hydrogels MC−co−MA have been studied in detail using dynamic light scattering DLS, scanning electron microscopy SEM, nuclear magnetic resonance (1H NMR) and rheology to evaluate the conformational changes, swelling–shrinkage, stability, the ability to flow and the diffusion process of nanoparticles at several temperatures. Furthermore, polymeric systems functionalized with acrylic acid MC and acrylamide MA were subjected to a titration process with a calcium chloride CaCl2 solution to analyze its effect on the average particle diameter Dz, polymer structure and the intra- and intermolecular interactions in order to provide a responsive polymer network that can be used as a possible nanocarrier for drug delivery with several benefits. The results confirmed that the structural changes in the sensitive hydrogels are highly dependent on the corresponding critical solution temperature CST of the carboxylic (–COOH) and amide (–CONH2) functional groups and the influence of calcium ions Ca2+ on the formation or breaking of hydrogen bonds, as well as the decrease in electrostatic repulsions generated between the polymer chains contributing to a particle agglomeration phenomenon. The temperature leads to a re-arrangement of the polymer chains, affecting the viscoelastic properties of the hydrogels. In addition, the diffusion coefficients D of nanoparticles were evaluated, showing a closeness among with the morphology, shape, size and temperature, resulting in slower diffusions for larger particles size and, conversely, the diffusion in the medium increasing as the polymer size is reduced. Therefore, the hydrogels exhibited a remarkable response to pH and temperature variations in the environment. During this research, the functionality and behavior of the polymeric nanoparticles were observed under different analysis conditions, which revealed notable structural changes and further demonstrated the nanoparticles promising high potential for drug delivery applications. Hence, these results have sparked significant interest in various scientific, industrial and technological fields.
Molecular Phylogeny of the Family Cordulegastridae (Odonata) Worldwide
Authors: Schneider Thomas , Vierstraete Andy , Kosterin Oleg E. , Ikemeyer Dietmar , Hu Fang-Shuo , Novelo-Gutiérrez Rodolfo , Kompier Tom , Everett Larry , Müller Ole , Dumont Henri J. 
Published: August 2024

In this study, we present the first attempt at a molecular phylogenetic analysis of the entire family of Cordulegastridae involving 60% of its known species. Our analysis is in favor of reclassification of the members of the family into four genera: (i) the monophyletic genus Anotogaster Selys, 1854, with the number of known species reduced by three synonymizations; (ii) the genus Cordulegaster Leach in Brewster, 1815 including all members of the boltonii group and, as a preliminary solution, the American species C. virginiae Novelo-Gutiérrez, 2018 and, very tentatively, C. diadema Selys, 1868. The bidentata group forms a genus of its own, for which we restored the name Thecagaster Selys 1854, stat. rev. Cordulegaster pekinensis McLachlan in Selys, 1886, currently considered as Neallogaster pekinensis, was placed by us in Thecagaster as well. The genus Neallogaster Cowley, 1934 needs further investigation involving all remaining species listed in it. The genus Zoraena Kirby, 1890, stat. rev., was recovered to accommodate the remaining American species of Cordulegaster. We synonymized three species of Anotogaster: Anotogaster gregoryi Fraser, 1923 = Anotogaster xanthoptera Lohmann, 1993, syn. nov.; Anotogasterkuchenbeiseri (Förster, 1899) = Anotogaster antehumeralis Lohmann, 1993, syn. nov.; Anotogaster kuchenbeiseri (Förster, 1899) = Anotogaster cornutifrons Lohmann, 1993, syn. nov., based on examination of the existing type specimens. The type of specimens of A. klossi Fraser, 1919 = A. flaveola Lohmann 1993 syn. confirm., were also examined, and their synonymy was confirmed. The isolated populations of A. sieboldii (Selys, 1854) from the archipelagos of Okinawa and Amami Oshima in Japan, respectively, should be regarded as separate species, which will be described elsewhere. Furthermore, we suggest the synonymization of Cordulegaster parvistigma Selys 1873 syn. nov. with Thecagaster brevistigma (Selys 1854) comb. restaur.
ATP, the 31P Spectral Modulus, and Metabolism
Authors: Greiner Jack V. , Glonek Thomas 
Published: August 2024

Adenosine triphosphate (ATP) has a high intracellular millimolar concentration (ca. 2.4 mM) throughout the phylogenetic spectrum of eukaryotes, archaea, and prokaryotes. In addition, the function of ATP as a hydrotrope in the prevention of protein aggregation and maintenance of protein solubilization is essential to cellular, tissue, and organ homeostasis. The 31P spectral modulus (PSM) is a measure of the health status of cell, tissue, and organ systems, as well as of ATP, and it is based on in vivo 31P nuclear magnetic resonance (31P NMR) spectra. The PSM is calculated by dividing the area of the 31P NMR integral curve representing the high-energy phosphates by that of the low-energy phosphates. Unlike the difficulties encountered in measuring organophosphates such as ATP or any other phosphorylated metabolites in a conventional 31P NMR spectrum or in processed tissue samples, in vivo PSM measurements are possible with NMR surface-coil technology. The PSM does not rely on the resolution of individual metabolite signals but uses the total area derived from each of the NMR integral curves of the above-described spectral regions. Calculation is based on a simple ratio of the high- and low-energy phosphate bands, which are conveniently arranged in the high- and low-field portions of the 31P NMR spectrum. In practice, there is essentially no signal overlap between these two regions, with the dividing point being ca. −3 δ. ATP is the principal contributor to the maintenance of an elevated PSM that is typically observed in healthy systems. The purpose of this study is to demonstrate that (1) in general, the higher the metabolic activity, the higher the 31P spectral modulus, and (2) the modulus calculation does not require highly resolved 31P spectral signals and thus can even be used with reduced signal-to-noise spectra such as those detected as a result of in vivo analyses or those that may be obtained during a clinical MRI examination. With increasing metabolic stress or maturation of metabolic disease in cells, tissues, or organ systems, the PSM index declines; alternatively, with decreasing stress or resolution of disease states, the PSM increases. The PSM can serve to monitor normal homeostasis as a diagnostic tool and may be used to monitor disease processes with and without interventional treatment.
Treatment results of lymphoblastic lymphomas from progenitor cells according to ALL IC-BFM 2002/2009 protocols: monocenter clinical trial
Authors: Pavlova T. Yu. , Valiev T. T. , Varfolomeeva S. R. 
Published: August 2024

Introduction. The acute lymphoblastic leukemia (ALL) therapy programs developed by the BFM group (Berlin-Frankfurt-Munster) are used to treat lymphoblastic lymphomas from precursor cells (LBL) and are the most effective treatment methods in the world.Aim. To assess the treatment results of LBL in children according to the ALL IC-BFM 2002/2009 protocols in a clinical trial of a single center.Materials and methods. From 2002 to 2022 a retrospective and prospective study included 70 patients aged 2 to 17 years with newly diagnosed LBL, with a median age of 9.4 years. There were 1.5 times more male patients than female (43:27). LBL from T-cells progenitor (T-LBL) was diagnosed in 61 patients (87 %), and LBL from B-cells progenitor (B-LBL) — in 9 (13 %). 59 (84.3 %) patients were included in the intermediate risk (IR) group and 11 (15.7 %) patients — in high-risk (HR) group. There were no patients in the study cohort who met the criteria of the standard risk group (SR).Results. The 10-year OS in the group of patients treated according to the ALL IC-BFM 2002/2009 protocols was 95.4 ± 2.6 %; the 10-year RFS — 91.9 ± 4.9 %; the 10-year EFS — 86.7 ± 5.6 %. When analyzing survival rates depending on the prognostic risk group, the 10-year OS for patients of the intermediate risk group was 96.6 ± 2.6 %, and for patients of the high-risk group — 90.9 ± 8.7 %.Conclusions. The obtained results confirm the high effectiveness of the ALL IC-BFM 2002/2009 protocols in the treatment of LBL.
The influence of the frequency of donor plasmapheresis on the concentration of ions and proteins in the blood of donors
Authors: Lamzin I. M. , Brylyaeva E. V. , Khapman M. E. , Goryachaya M. N. 
Published: August 2024

Introduction. Plasmapheresis is a routine procedure in many blood centers. The most common complications in plasma donors are vasovagal and citrate reactions. The effects of citrate exposure, repeated with different frequency over a long period of time, have not been sufficiently studied.Aim. to research the effect of the frequency of plasmapheresis on the concentration of ionic and protein composition in donor blood.Materials and methods. The concentration of protein in blood serum was measured using a biochemical method. The concentration of ions was carried out using an automatic blood electrolyte analyzer. Plasma donors were divided into groups depending on gender and frequency of plasmapheresis (1–5 times a year; 6–10 times a year; 11–26 times a year). The total number of donors involved in the study was 274 (155 men and 119 women).Results. A significant decrease in the concentration of bound calcium was revealed in the group of female donors who donated plasma 11–26 times compared to the group who donated plasma 1–5 times a year. A significant decrease in the concentration of iron ions was revealed in the group of female donors who donated plasma 11–26 times compared to the group who donated plasma 1–5 times a year. Statistically significant differences in the concentrations of total protein were revealed between all three groups of male donors, and between groups of female donors who donated plasma 1–5 times and 6–10 times a year; 1–5 times and 11–26 times a year.Conclusion. The use of data on changes in the parameters of ion and protein metabolism in donors of different sexes and different donation frequencies reduces the risk of developing a citrate reaction due to the selective selection of plasma donors.
Features of the T-cell immune response in patients with hematological diseases after SARS-CoV-2 infection and vaccination
Authors: Zornikova K. V. , Ivanova N. O. , Aleshina O. A. , Sheetikov S. A. , Davydova V. D. , Bogolyubova A. V. 
Published: August 2024

Introduction. Patients with hematological malignancies are one of the most vulnerable groups to infectious diseases. Immune system dysfunction caused by the disease and/or its therapy negatively affects infection disease duration and severity, leads to an increased risk of death, and reduces the efficacy of vaccination. However, the precise compensatory mechanisms that allow patients with deficient cellular or humoral responses to fight viral infections have not been sufficiently studied.Aim: To systematize the knowledge of immune response formation in patients with hematological malignancies with humoral or cellular dysfunction.Main findings. In this review the features of the T-cell immune response in patients with hematological malignancies is explored, followed by a discussion on how the diversity of the T-cell receptor repertoire may be important in effective virus elimination and protection against SARS-CoV-2 virus infection.
Thrombosis in patients with inherited coagulation factor disorders
Authors: Florinskiy D. B. , Zharkov P. A. 
Published: August 2024

Introduction. The main manifestation of hereditary coagulopathies is bleeding, but patients with these diseases are not protected from thrombotic complications.Aim: to evaluate the frequency and nature of thrombotic complications in various bleeding disorders.Main findings. In some bleeding disorders, such as von Willebrand disease, thromboses are extremely rare, while in others, such as dysfibrinogenemias, thrombosis is one of the key manifestations. For hemophilia A, there has recently been a significant expansion of treatment options. The use of some new drugs has already been associated with thrombotic complications in the clinical trial phase.
Radiosynovectomy for hemophilic arthropathies. Analysis of opportunities and prospects
Authors: Krylov V. V. , Zheleznov A. A. , Zorenko V. Yu. , Kim A. Yu. 
Published: August 2024

Introduction. A characteristic symptom of hemophilia is joint hemorrhage (hemarthroses).Aim: to evaluate the possibilities of radiosynovectomy in hemophilic arthropathies.Main findings. Radiosynovectomy (RSE) or radiosynoviorthesis (RSO) is a technique that achieves a long-lasting effect in chronic synovitis and hemophilic arthropathy. It consists of intra-articular injection of radiopharmaceuticals (RFLP) in the form of colloidal solutions, macroaggregates or microspheres containing radioactive isotopes, which are rapidly phagocytized by the covering cells of the synovial membrane. After such capture, the synovial sheath is subjected to intensive “internal” irradiation, which results in superficial fibrosis of synovial tissue and persistent suppression of joint inflammation.
Successful treatment of acute promyelocytic leukemia with initial hyperleukocytosis, differentiated syndrome and massive intracranial hemorrhage in a child
Authors: Goryacheva S. V. , Batmanova N. A. , Valiev T. T. , Matinyan N. V. , Kashanina A. L. , Smirnova D. S. 
Published: August 2024

Introduction. The development of hemorrhagic syndrome at the onset of the disease in patients with acute promyelocytic leukemia (APL) is one of the main causes of therapy failures and the most common cause of death in the early stages of remission induction. The risk of severe hemorrhagic complications increases in patients with the microgranular APL variant, often accompanied with hyperleukocytosis.Aim — to present a clinical case of successful diagnosis and treatment of primary APL with initial hyperleukocytosis in a patient with massive intracranial hemorrhage and differentiated syndrome.Main findings. A clinical observation of the diagnosis and treatment of APL in a 13-year-old patient with initial hyperleukocytosis, massive intracranial hemorrhage and differentiation syndrome is presented. Data on the variable manifestations of hemorrhagic syndrome in patients with APL and its effect on survival outcomes are also given. Diagnostic criteria and approaches in the treatment of differentiation syndrome are presented on the example of a clinical observation, when early clinical diagnosis and timely initiation of special antitumor treatment in the intensive care unit made it possible to achieve clinical, hematological and molecular remission, avoiding the fatal consequences of massive intracranial hemorrhage.
Thrombophilia as a reason for the development of ischemic stroke at young age. Features of the clinic and diagnosis
Authors: Savchuk E. A. , Melnichenko S. A. , Kurtadzhieva A. V. , Savchuk E. O. , Maksimova P. E. , Zyablitskaya E. Yu. 
Published: August 2024

Introduction. Thrombophilia is a common cause of neurological complications.Aim. to present a clinical observation of the development of ischemic stroke in a patient with hereditary thrombophilia.Main findings. A case is presented of a man, aged 30, who developed an ischemic stroke in the basin of the left medial artery. A diagnostic study revealed that the development of the ischemic stroke was facilitated by thrombophilia caused by a combination of polymorphic variants of the fibrinogen genes (G-455A(G-467A)) and methylenetetrahydrofolate reductase (C677T). Thus, hereditary thrombophilia was a risk factor for the development of ischemic stroke in the patient at a young age. Therefore, in the absence of traditional risk factors for the development of ischemic stroke, a genetic analysis for thrombophilia is necessary.
Faktor Risiko Kejadian Stunting pada Balita di Desa Gelangsar Kecamatan Gunung Sari
Authors: Mardiah Aena , Karmila Dany , Utami Sukandriani , Anulus Ayu 
Published: August 2024

Stunting merupakan masalah global, termasuk Indonesia dan menduduki posisi kelima terbanyak dalam jumlah stunting di dunia. Prevalensi stunting di Indonesia tahun 2019 sebesar 27,67 %. Kabupaten/ Kota di Provinsi Nusa Tenggara Barat dengan prevalensi stunting tertinggi yaitu di Kabupaten Lombok Tengah sebesar 45,25 %. Stunting disebabkan oleh faktor multi dimensi yaitu penyebab langsung, tidak langsung dan penyebab mendasar. Tujuan penelitian adalah menganalisis faktor risiko kejadian stunting pada balita di Desa Gelangsar Kecamatan Gunung Sari Kabupaten Lombok Barat. Penelitian ini merupakan penelitian observasional dengan metode rancangan Cross sectional. Penelitian ini dilakukan di Desa Gelangsar Kecamatan Gunung Sari karena merupakan salah satu desa dengan prevalensi stunting yang tinggi di Kabupaten Lombok Barat Provinsi Nusa Tenggara Barat. Pelaksanaan penelitian dilakukan pada bulan Maret 2021. Subjek penelitian ini adalah anak usia 0 – 60 bulan sebanyak 136 responden. Instrumen yang digunakan dalam penelitian adalah: kuesioner dan lembar observasional. Analisis data menggunakan analisis univariat, analisis bivariat menggunakan uji statistik chi square. Hasil bivariat menunjukkan berat badan bayi lahir rendah (p-value=0,007;RP=2,61;CI=1,60-4,28). Faktor demografi (pendidikan ayah (p-value=0,59;RP=1,19;CI=0,62-2,31), pendidikan ibu (p-value=0,28;RP=1,42;CI=0,73-2,77), dan pekerjaan ibu (p-value=0,57;RP=1,61;CI=0,27-9,48)), faktor praktek perawatan ibu dan anak (pemberian asi eksklusif (p-value=0,17;RP=0,33;CI=0,52-2,15), pemberian MP-ASI (p-value=0,06;RP=1,51;CI=1,32-1,71)), faktor ketersediaan jamban (p- value=0,59;RP=0,77;CI=0,28-2,13), imunisasi (p-value=0,44;RP=0,74;CI=0,40-1,37) dan riwayat penyakit infeksi diare (p-value=0,43;RP=1,23;CI=0,73-2,08) dan ISPA (p-value=0,09;RP=3,73;CI=0,57-24,62) tidak berhubungan dengan kejadian stunting pada balita. Berat badan bayi lahir rendah berhubungan dengan kejadian stunting pada balita di Desa Gelangsar Kecamatan Gunung Sari