bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor-interacting protein kinase 2 promotes triple-negative breast cancer cell migration and invasion via activation of nuclear factor-kappaB and c-Jun N-terminal kinase pathways
Authors: Singel Stina M , Batten Kimberly , Cornelius Crystal , Jia Gaoxiang , Fasciani Gail , Barron Summer L , Wright Woodring E , Shay Jerry W 
Published: March 2014

Abstract
Introduction
Metastasis is the main cause of breast cancer morbidity and mortality. Processes that allow for tumor cell migration and invasion are important therapeutic targets. Here we demonstrate that receptor-interacting protein kinase 2 (RIP2), a kinase known to be involved in inflammatory processes, also has novel roles in cancer cell migration and invasion.

Methods
A total of six breast cancer expression databases, including The Cancer Genome Atlas, were assessed for RIP2 expression among various clinical subtypes and its role as a prognostic biomarker. mRNA fluorescence in situ hybridization (FISH) for RIP2 was performed on 17 stage III breast cancers to determine if there was a correlation between RIP2 expression and lymph node involvement. RNA-interference was used to knock-down RIP2 expression in MDA-MB-231, Htb126, SUM149PT, MCF7, T47D, and HCC1428 cells. Cell migration and invasion were measured in vitro by scratch/wound healing and transwell migration assays. A xenograft mouse model was used to assess tumor growth and chemosensitivity to docetaxel in vivo in MDA-MB-231 cells with and without RIP2 small hairpin RNA knockdown. Western blot and immunofluorescence imaging were used to evaluate protein expressions.

Results
Interrogation of expression databases showed that RIP2 expression is significantly over-expressed in triple-negative breast cancers (TNBC: estrogen-receptor (ER) negative, progesterone-receptor (PR) negative, Her2/neu- (Her2) negative), compared to other clinical subtypes. High RIP2 expression correlates with worse progression-free survival using a combined breast cancer expression array dataset consisting of 946 patients. Multivariate analysis shows RIP2 as an independent prognostic biomarker. Knock-down of RIP2 significantly decreases migration in both scratch/wound healing and transwell migration assays in MDA-MB-231, Htb126, SUM149PT, MCF7, and T47D cells and is correlated with decreased Nuclear Factor-kappaB and c-Jun N-terminal kinase (JNK) activation. Finally, RIP2 knock-down leads to increased sensitivity to docetaxel and decreased tumor mass and lung metastases in a xenograft mouse model.

Conclusion
These results highlight RIP2 as a pro-metastasis kinase in patients with advanced breast cancer. These results also illustrate a novel role for this kinase in addition to its known role in inflammation, and suggest that targeting RIP2 may improve outcomes in advanced breast cancer patients, in which it is overexpressed.
Evidence for Decreased Expression of ADAMTS-1 Associated With Impaired Oocyte Quality in PCOS Patients
Authors: Xiao Shan , Li Yubin , Li Tao , Chen Minghui , Xu Yanwen , Wen Yangxing , Zhou Canquan 
Published: March 2014

Context:
Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may be altered in PCOS granulosa cell (GC)-reflecting abnormalities in ovulatory signaling.

Objective:
The purpose of this paper is to analyze the differential expression of ADAMTS-1 in PCOS patients associated with impaired oocyte quality.

Design and Setting:
A prospective comparative experimental study was performed at a clinical reproductive medicine center.

Patients:
Women with PCOS (n = 40) and normovulatory controls (n = 40) undergoing controlled ovarian hyperstimulation and in vitro fertilization were recruited in our study.

Main Outcome Measures:
Differential expression of ADAMTS-1 in GCs was analyzed with immunocytochemistry in PCOS patients and normal controls, and ADAMTS-1 mRNA expression was quantified by RT-PCR. Furthermore, the correlation between ADAMTS-1 mRNA and oocyte quality was analyzed.

Results:
The expression of ADAMTS-1 was decreased in PCOS patients compared with normally ovulating women and was closely related to lower oocyte recovery, oocyte maturity, and fertilization rate.

Conclusion:
Our study provides evidence that the dysregulated expression of ADAMTS-1 in PCOS may influence oocyte quality-via GCs-oocyte paracrine and endocrine mechanism.
Minireview: The Versatile Roles of Lysine Deacetylases in Steroid Receptor Signaling
Authors: Kadiyala Vineela , Smith Catharine L. 
Published: March 2014

AbstractLysine deacetylases have been known to regulate nuclear receptor function for many years. In the unliganded state, nuclear receptors that form heterodimers with retinoid X receptors, such as the retinoic acid and thyroid hormone receptors, associate with deacetylases to repress target genes. In the case of steroid receptors, binding of an antagonist ligand was initially reported to induce association of deacetylases to prevent activation of target genes. Since then, deacetylases have been shown to have diverse functions in steroid receptor signaling, from regulating interactions with molecular chaperones to facilitating their ability to activate transcription. The purpose of this review is to summarize recent studies on the role of deacetylases in steroid receptor signaling, which show deacetylases to be highly versatile regulators of steroid receptor function.
An Operational Application of NWP Models in a Wind Power Forecasting Demonstration Experiment
Authors: Yu Wei , Plante André , Dyck Sarah , Chardon Laurent , Forcione Alain , Choisnard Julien , Benoit Robert , Glazer Anna , Roberge Gaétan , Petrucci Franco , Bourret Jacques , Antic Slavica 
Published: March 2014

Environment Canada (EC) and Hydro-Québec (HQ) have been collaborating in a Research & Development and Demonstration project on a high resolution wind energy dedicated forecasting system (SPÉO: Système de Prévision ÉOlien under its French acronym). This project emphasizes the operational tests and the forecast of high impact events, e.g. wind ramps. It was found that SPÉO improves the Canadian Regional Deterministic Prediction System (RDPS), by about 18% in terms of the RMSE (Root Mean Square Error) of the predicted wind speed when compared with mast observations from three wind power plants. The improvement is most significant in the cold season. When the average wind speed measured at all wind turbines (nacelle anemometer) is used as a reference, SPÉO improves the RMSE of the average wind speed at a wind power plant in complex terrain (24%) compared with that of RDPS. However, there is almost no improvement for two other wind power plants located in less complex terrain. The average wind speed is corrected with the average wind speed measured at all turbines, and is then fed into a wind-to-power conversion module for power production forecasts. The power production forecast is improved by 6% on average in complex terrain when SPÉO winds are used as input compared to the RDPS. The most important finding of this project is SPÉO's ability to predict ramps due to mountain waves/downslope winds. The proposed forecast index for ramps based on the Froude number is useful for predicting the onset of this kind of ramp when a high resolution NWP model is unavailable.
Modern lesson at primary school taking into account fses requirements
Authors: Белорыбкина  , Belorybkina Elena 
Published: March 2014

Changes which should happen in purposes, contents and technology of primary school lesson taking into account teaching’s planned results
determined by the Federal State Educational Standard of the Primary General Education (FSES of PGE) are considered in this paper. New
approach to organization of junior school pupil’s activity at a lesson is revealed: this is a use of choice method, &#34;inclusion&#34; situations, etc.
New approaches to teaching of junior school pupils in literary reading
Authors: Хомякова  , Khomyakova Irina 
Published: March 2014

In this paper a value of &#34;Literary Reading&#34; subject for achievement of planned results related to the Federal State Educational Standard of the
Primary General Education (FSES of PGE) — formation of educational and informative motivation, development of universal educational
actions is revealed. Concrete examples related to methods of work with junior school pupils at literary reading lessons are given.
Medienbildung als gemeinsamer Bildungsauftrag von Bibliothek und Schule
Authors: Lücke Birgit 
Published: March 2014

Zusammenfassung:
Seit zwölf Jahren arbeitet die Stadtbücherei Warendorf im Bereich der Medienbildung eng mit allen Schulen und Kindertagesstätten am Ort zusammen. Der Praxisbericht zeigt auf, wie es gelungen ist, Medienbildung als gemeinsamen Bildungsauftrag zu gestalten, wo Gefahren lauern und wie man sie umgehen kann. Zur Zeit entsteht in Anlehnung an den Medienpass NRW der Medienpass Warendorf, der mit seinen Angeboten mehr als bisher den Fachunterricht direkt in der Schule unterstützen soll und darum online verfügbare Elemente wie Datenbanken und eLearning-Komponenten ebenso einbezieht wie Medienbestände in den Schulen.
The Effect Tool Geometry on Resonance Phenomenon in Turning Process
Authors: Mahdavinejad R. A. , Khajeafzali M. 
Published: March 2014

Abstract
The determination and optimization of applied forces on cutting tool is usually one of the most important subject to determine the tool's long lasting and break horse power of machine tool. In This research the effect of rake angle and cutting depth on the force applied to cutting tool in turning process is analyzed via the investigation of forces in their frequency domain. From This point of view, the applied forces to cutting tool are experimentally measured in three directions via dynamometer. After that, the spectrum frequencies of these forces are analyzed with Fourier transformation. Finally, the effect of machining parameters on resonance phenomenon is investigated via the determination of natural frequency of cutting tool. Due to the variations of tool geometry and machining parameters based on the dynamic behavior, the forces can be controlled with resonance phenomenon and also reduction in brake horsepower.
Optimization of Machining Parameters in ECM of Al/B4C Composites
Authors: Rama Rao Sadineni , Padmanabhan G. 
Published: March 2014

AbstractThe present paper deals the application of Taguchi method with grey relational analysis to optimize the machining parameters with multiple responses in electrochemical machining (ECM) of Al/B4C metal matrix composites. Experiments were conducted on a Metatech ECM setup and L27 orthogonal array was chosen for the experiments. The electrochemical machining parameters namely applied voltage, feed rate, electrolyte concentration and reinforcement content were optimized based on multiple responses, i.e. material removal rate, surface roughness and radial over cut. The morphology of the machined surfaces was studied with scanning electron micrographs. The optimum machining parameters are calculated by using grey theory and results are compared with ANOVA. The results show that the feed rate and electrolyte concentration are the most significant parameters which affect the multiple machining responses simultaneously. Experimental results show that the responses in ECM can be improved effectively through this approach.
Optimization of Flux Cored Arc Welding Process Parameter Using Genetic and Memetic Algorithms
Authors: Kannan T. , Murugan N. , Sreeharan B. N. 
Published: March 2014

AbstractMost of the manufacturing enterprises indulge in the bonding of metals during the production process. This makes welding one of the most important processes in industries. Subsequently, due to the high usage of welding process, industrial engineers desire to optimize the parameters concerned to achieve the desired weld bead characteristics. This paper focuses on optimization of flux cored arc welding process parameters, which are used for deposition of duplex stainless steel on low carbon structural steel plates. Experiments were conducted based on central composite rotatable design and mathematical models were developed using multiple regression method. Further, optimization with objectives as minimizing percentage dilution, maximizing height of reinforcement and bead width was carried out using genetic algorithm and memetic algorithm. This problem was formulated as a multi objective, multivariable and non-linear programming problem. The algorithms were implemented using basic functions of C language making it highly reliable, adoptable, very user friendly and extendable to other welding processes such as GMAW, GTAW, robotic welding, etc. The adopted optimization techniques were further compared based on various computational factors.
Manufacturing Dies for Micro and Meso Direct Extrusion Processes
Authors: Milanez Alexandre , Schaeffer Lírio , Daleffe Anderson , Milanez Mateus 
Published: March 2014

AbstractThis work describes the development and manufacture of micro and meso extrusion dies used to extrude metal materials. The die design was based on different extrusion angles and diameters of input materials. An extrusion die was made to input material measuring 1 mm and for reduction to 0.8 mm with a 30° angle of extrusion. For the same diameter of material, another two dies were manufactured with extrusion angles of 45° and 60°. For meso sized extrusion, another three dies were made and the diameter of the input material was 4 mm with a reduction to 3 mm and extrusion angles of 30°, 45° and 60°. During the machining of the extrusion dies, high speed steel (HSS) drills were used with a tip angle according to the extrusion angles. After machining, the dies were treated thermally and polished to diminish the friction coefficient between the part to be extruded and the die. The results of the machining process indicate that extrusion dies can be manufactured using quick HSS drill and polishing can be done using diamond paste with satisfactory results.
A Study on Dimensional Accuracy of Fused Deposition Modeling (FDM) Processed Parts using Fuzzy Logic
Authors: Sahu Ranjeet Kumar , Mahapatra S.S. , Sood Anoop Kumar 
Published: March 2014

AbstractFused Deposition Modeling (FDM) is an additive manufacturing technology for rapid prototyping that can build intricate parts in minimal time with least human intervention. The process parameters such as layer thickness, orientation, raster angle, raster width and air gap largely influence on dimensional accuracy of built parts which can be expressed as change in length, width and thickness. This paper presents experimental data and a fuzzy decision making logic in integration with the Taguchi method for improving the dimensional accuracy of FDM processed ABSP 400 parts. It is observed that length and width decreases but thickness shows positive deviation from desired value of the built part. Experimental results indicate that optimal factor settings for each response are different. Therefore, all the three responses are expressed in a single response index through fuzzy logic approach. The process parameters are optimized with consideration of all the performance characteristics simultaneously. Finally, an inference engine is developed to perform the inference operations on the rules for fuzzy prediction model based on Mamdani method. Experimental results are provided to confirm the effectiveness of the proposed approach. The predicted results are in good agreement with the values from the experimental data with average percentage error of less than 4.5.
Gold nanoparticles-coated chemically-reactive polymer colloids and the study of their catalytic kinetics
Authors: Li Maolin , Zhong Vivian , Chen Guofang 
Published: March 2014

ABSTRACTRaspberry-like composite spheres based on chemically-reactive poly(glycidyl methacrylate) (PGMA) colloids as the cores coated with tunable size of gold nanoparticles were synthesized via a controlled assembly method. Kinetic study of 4-nitrophenol reduction by NaBH4 in the presence of poly(allylamine hydrochloride)-modified PGMA composite with tunable size of AuNPs (PGMA@PAH@AuNPs) was demonstrated. Effects of gold nanoparticles size and PGMA colloid diameter on the reaction time, average reaction rate and average turnover frequency (TOF), order of reaction (n) and apparent rate constant (kapp) were systematically investigated. Experimental results of our study showed composites with 3.4 ± 0.9 nm AuNPs have the best catalytic efficiency with the highest reaction order and apparent rate constant. The poisoning of product 4-aminophenol on PAH-modified PGMA colloid-supported gold nanocatalysts was evaluated using 4-nitrophenol/NaBH4 reduction reaction for the reaction time, average reaction rate, average TOF, order of reaction and apparent rate constant.
Solution Plasma Synthesis of Nitrogen-Doped Carbon Nanoballs as Effective Metal-Free Electrocatalysts for Oxygen Reduction Reaction
Authors: Panomsuwan Gasidit , Chiba Satoshi , Saito Nagahiro , Ishizaki Takahiro 
Published: March 2014

ABSTRACTNitrogen-doped carbons nanoballs were synthesized from an organic liquid precursor (a mixture of benzene and pyrazine) by solution plasma process. After synthesis, they were further annealed at 700 and 900 °C under N2 atmosphere. The nitrogen-doped carbon nanoballs before and after thermal annealing process exhibit a similar morphological feature, and their diameters are in the range between 20 and 40 nm. With higher annealing temperature, the graphitization of the nitrogen-doped carbon nanoballs increases. For the electrocatalytic activity in an alkaline solution, the limiting current density and onset potential for the ORR activity can be significantly improved for the samples after thermal annealing at 900 °C. We anticipate that solution plasma process will be a viable alternative way for synthesizing heteroatom-doped carbon electrocatalysts for broad application in the field of fuel cells, metal-air batteries, and supercapacitors.
Helium Implantation of Ultrafine Grained Tungsten within a TEM
Authors: Hattar K. , El-Atwani O. , Efe M. , Novakowski T.J. , Suslova A. , Allain J.P. 
Published: March 2014

ABSTRACTMany theoretical predictions have suggested that the confined length scales and increased interface density of various nanostructured materials may result in desired thermal, mechanical, and radiation properties. An important aspect of this for next generation nuclear reactors is understanding the change in swelling resulting from helium evolution in tungsten alloys, as a function of grain size and grain boundary type. This study investigated this using a new ion irradiation transmission electron microscope (TEM) facility that has been developed at Sandia National Laboratories and is capable of ion implanting helium at energies up to 20 keV. It was demonstrated in this feasibility study that helium could be implanted into an ultrafine grained tungsten TEM sample produced by severe plastic deformation. The size and density of the helium bubbles formed during the experiment appear nearly constant; while the larger voids formed appear to be dependent on the local microstructure. Future work is underway to both optimize the facility, as well as better understand the evolution of ultrafine grained tungsten resulting from both helium implantation and displacement damage.
Comparison of Theoretical Approaches Predicting the Coherent-Semicoherent Transition in Nanoscale Axial Heterostructures
Authors: Riedl Thomas , Lindner Joerg K.N. 
Published: March 2014

ABSTRACTThe formation of misfit dislocations is an important issue for the performance of heteroepitaxial micro- and optoelectronic devices. We analyze three approaches that quantify the stability of misfit dislocations in axial-heteroepitaxial nanowires with respect to applicability and predictions of critical nanowire dimensions. The “nanoheteroepitaxy” approach of Zubia and Hersee proves suitable for determination of strain partitioning in the presence of an elastic mismatch. Concerning the critical thickness and diameter however the descriptions of Ertekin et al. and Glas respectively yield more reliable results, owing to the consideration of the total coherent and dislocation related energies plus the residual strain energy. In contrast to the model of Ertekin et al., which refers to infinitely long nanowires, the other two mentioned approaches allow predictions of the critical thickness of mismatched deposits on the nanowire axial face.
Mechanical-assisted preparation and photocatalytic properties of almost-visible light-driven ZnO/ZnFe2O4 nanocomposites
Authors: Qin Jiaqian , Xue Yanan , Zhang Xinyu , Liu Riping 
Published: March 2014

ABSTRACTZnO and ZnO/ZnFe2O4 nanocomposites were synthesized by mechanical-assisted thermal decomposition method. The results show that this method is a simple and low cost method to prepare ZnO and ZnO/ ZnFe2O4 nanocomposites. The nanorod's shape and size have been identified through SEM and TEM. The photocatalytic activity of ZnO and ZnO/ZnFe2O4 nanocomposites were tested by the degradation of methylene blue (MB) in aqueous medium under almost-visible light and the efficiency of the catalyst has been discussed in detail.
LSM Protective Coatings on Stainless Steel as Interconnects for Solid Oxide Fuel Cells
Authors: Eriksen Ryan , Gopalan Srikanth , Sampath Sanjay , Chen Yikai 
Published: March 2014

ABSTRACTOne of the major barriers to the adoption of solid oxide fuel cells (SOFCs) is the short lifetime of the fuel cell stacks. A stack consists of a number of cells in series separated by an interconnect. Due to the high temperatures necessary for SOFCs, typical commercial interconnects are ceramic. Great attention has been paid to decreasing the operating temperature of SOFCs in order to extend the life and decrease the cost of the stack. As operating temperatures decrease below 1000°C, alternative interconnect materials become viable. Stainless steel interconnects are more cost effective than ceramic interconnects but the high temperatures and the oxidizing environment of the cathode leads to the formation of a chromium oxide scale that increases the stack resistance. Chromium from the stainless steel can also enter the vapor phase and redeposit on the cathode thereby blocking the electrochemically active sites. One method to neutralize these effects is to coat the metallic interconnect in a ceramic such as La.8Sr.2MnO3 (LSM). The coating acts as a diffusion barrier both against chromium diffusing into the cathode and oxygen diffusing into the interconnect. In this study LSM has been deposited using plasma spray and tested in a dual atmosphere setup using impedance spectroscopy to analyze the performance of the coatings at various temperatures. The area specific resistance and chemical composition of the scale was examined in order to determine the affect of the LSM coating.