bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of a congested fixture period on physical performance, technical activity and injury rate during matches in a professional soccer team
Authors: Dellal Alexandre , Lago-Peñas Carlos , Rey Ezequiel , Chamari Karim , Orhant Emmanuel 
Published: February 2013

BackgroundIn modern professional soccer, the ability to recover from official match-play and intense training is often considered a determining factor in subsequent performance.ObjectiveTo investigate the influence of playing multiple games with a short recovery time between matches on physical activity, technical performance and injury rates.MethodsThe variation of physical (overall distance, light-intensity, low-intensity, moderate-intensity and high-intensity running) and technical performance (successful passes, balls lost, number of touches per possession and duels won) of 16 international players was examined during three different congested periods of matches (six games in 18 days) from the French League and Cup (n=12), and the UEFA Champions’ League (n=6) during the 2011–2012 season and compared with that reported in matches outside these periods. Data were collected using a computerised match analysis system (Amisco). Injury rate, time loss injuries, as well as the mechanism, circumstances and severity of the injury were also analysed.ResultsNo differences were found across the six successive games in the congested period, and between no congested and the three congested periods for all the physical and technical activities. The total incidence of injury (matches and training) across the prolonged congested periods did not differ significantly to that reported in the non-congested periods. However, the injury rate during match-play was significantly higher during the congested period compared with the non-congested period (p<0.001). The injury rate during training time was significantly lower during the congested period compared with the non-congested periods (p<0.001). The mean lay-off duration for injuries was shorter during the congested periods compared with the non-congested periods (9.5±8.8 days vs 17.5±29.6 days, respectively p=0.012, effect sizes=0.5).ConclusionsAlthough physical activity, technical performance and injury incidence were unaffected during a prolonged period of fixture congestion, injury rates during training and match-play and the lay-off duration were different to that reported in matches outside this period.
CYP24A1 and CYP27B1 Polymorphisms Modulate Vitamin D Metabolism in Colon Cancer Cells
Authors: Jacobs Elizabeth T. , Van Pelt Chad , Forster Ryan E. , Zaidi Wasiq , Hibler Elizabeth A. , Galligan Michael A. , Haussler Mark R. , Jurutka Peter W. 
Published: February 2013

Abstract
Vitamin D is a well-studied agent for cancer chemoprevention and treatment. Its chief circulating metabolite, 25-hydroxyvitamin D, is converted into the active hormone 1,25-dihydroxyvitamin D (1,25D) by the cytochrome P450 enzyme CYP27B1 in kidney and other tissues. 1,25D is then deactivated by CYP24A1 and ultimately catabolized. Colorectal carcinoma cells express CYP27B1 and CYP24A1 that locally regulate 1,25D with potential implications for its impact on carcinogenesis. While 1,25D inhibits cancer growth, the effects of polymorphic variations in genes encoding proteins involved in 1,25D homeostasis are poorly understood. Using an RXR-VDR mammalian two-hybrid (M2H) biologic assay system, we measured vitamin D metabolite uptake and activation of the vitamin D receptor (VDR) pathway in colon cancer cells that expressed one of five CYP27B1 single-nucleotide polymorphisms (SNP) or four CYP24A1 SNPs. Compared with the wild-type control, four of five CYP27B1 SNPs reduced enzymatic activity, whereas one (V166L) increased activity. For CYP24A1, all tested SNPs reduced enzyme activity. Quantitative real-time PCR analyses supported the results of M2H experiments. The observed SNP-directed variation in CYP functionality indicated that vitamin D homeostasis is complex and may be influenced by genetic factors. A comprehensive understanding of 1,25D metabolism may allow for a more personalized approach toward treating vitamin D–related disorders and evaluating risk for carcinogenesis. Cancer Res; 73(8); 2563–73. ©2013 AACR.
Bexarotene Induces Cellular Senescence in MMTV-Neu Mouse Model of Mammary Carcinogenesis
Authors: Shilkaitis Anne , Bratescu Laura , Green Albert , Yamada Tohru , Christov Konstantin 
Published: February 2013

Abstract
Previous studies have shown that retinoids and rexinoids can prevent breast cancer in animal models and in women with increased risk of developing the disease. The cellular effects of these vitamin A analogues have been primarily associated with induction of differentiation and inhibition of proliferation. In this study, we tested the hypothesis that bexarotene (LGD1069, Targretin), a rexinoid, can not only inhibit cell proliferation but also induce cellular senescence in mammary epithelial cells, premalignant lesions, and tumors of the MMTV-Neu model of mammary carcinogenesis, which develops estrogen receptor–negative tumors. Mice with palpable mammary tumors were treated for 4 weeks with bexarotene at 80 or 40 mg/kg body weight, and senescent cells were determined by SA-β-Gal assay. Bexarotene decreased in a dose-dependent manner the multiplicity of premalignant lesions and tumors, and this was associated with inhibition of cell proliferation and induction of cellular senescence and apoptosis. By double labeling of senescent cells, first by SA-β-Gal and then by antibodies against genes related to cellular senescence, we found that p21, p16, and RARβ, but not p53, were upregulated by bexarotene in mammary tumors and in breast cancer cell lines, suggesting involvement of multiple signaling pathways in mediating the senescence program of rexinoids. These findings indicate that, in addition to cell proliferation and apoptosis, cellular senescence could be used as a potential biomarker of response in breast cancer prevention and therapy studies with rexinoids and possibly with other antitumor agents. Cancer Prev Res; 6(4); 299–308. ©2013 AACR.
Independent Risk Factors for Intracranial Aneurysms and Their Joint Effect
Authors: Vlak Monique H.M. , Rinkel Gabriel J.E. , Greebe Paut , Algra Ale 
Published: February 2013

Background and Purpose—
Three percent of the population has an unruptured intracranial aneurysm (UIA). We aimed to identify independent risk factors from lifestyle and medical history for the presence of UIAs and to investigate the combined effect of well-established risk factors.

Methods—
We studied 206 patients with an UIA who never had a subarachnoid hemorrhage and 574 controls who were randomly retrieved from general practitioner files. All participants filled in a questionnaire on potential risk factors for UIAs. With logistic regression analysis, we identified independent risk factors for UIA and assessed their combined effect.

Results—
Independent risk factors were current smoking (odds ratio [OR], 3.0; 95% confidence interval [CI], 2.0–4.5), hypertension (OR, 2.9; 95% CI, 1.9–4.6), family history of stroke other than subarachnoid hemorrhage (OR, 1.6; 95% CI, 1.0–2.5), hypercholesterolemia (OR, 0.5; 95% CI, 0.3–0.9), and regular physical exercise (OR, 0.6; 95% CI, 0.3–0.9). The joint risk of smoking and hypertension was higher (OR, 8.3; 95% CI, 4.5–15.2) than the sum of the risks independently.

Conclusions—
Current smoking, hypertension, and family history of stroke increase the risk of UIA, with smoking and hypertension having an additive effect, whereas hypercholesterolemia and regular physical exercise decrease this risk. A healthy lifestyle probably reduces the risk of UIA and thereby possibly also that of aneurysmal subarachnoid hemorrhage. Whether smoking and hypertension increase the risk of aneurysmal subarachnoid hemorrhage only through an increased risk of aneurysm formation or also through an increased risk of rupture remains to be established.
Patient-Reported Measures Provide Unique Insights Into Motor Function After Stroke
Authors: Stewart Jill Campbell , Cramer Steven C. 
Published: February 2013

Background and Purpose—
Patient-reported outcome measures have been found useful in many disciplines but have received limited evaluation after stroke. The current study investigated the relationship that patient-reported measures have with standard impairment and disability scales after stroke.

Methods—
Patients with motor deficits after stroke were scored on standard assessments including the National Institutes of Health Stroke Scale, modified Rankin Scale, and Fugl-Meyer motor scale, and on 2 patient-reported measures, the hand function domain of the Stroke Impact Scale, which documents difficulty of hand motor usage, and the amount of use portion of the Motor Activity Log, which records amount of arm motor usage.

Results—
The 43 participants had mild disability (median modified Rankin Scale=2), moderate motor deficits (Fugl-Meyer motor scale=46±22), and mild cognitive/language deficits. The 2 patient-reported outcome measures, Stroke Impact Scale and Motor Activity Log, were sensitive to the presence of arm motor deficits. Of 21 patients classified as having minimal or no impairment or disability by the National Institutes of Health Stroke Scale or modified Rankin Scale (score of 0–1), 15 (71%) reported difficulty with hand movements by the Stroke Impact Scale score or reduced arm use by the Motor Activity Log score. Furthermore, of 14 patients with a normal examination, 10 (71%) reported difficulty with hand movements or reduction in arm use.

Conclusions—
Patient-reported measures were a unique source of insight into clinical status in the current population. Motor deficits were revealed in a majority of patients classified by standard scales as having minimal or no disability, and in a majority of patients classified as having no deficits.
Jesus Traditions and Masculinities in World Christianity
Authors: van Klinken Adriaan S. , Smit Peter-Ben 
Published: February 2013

Abstract
This opening article offers an introduction to the theme of this special issue of Exchange: Jesus traditions and masculinities in world Christianity. Highlighting the historical trajectory of feminist theological debates on the maleness of Jesus Christ and its implications for configurations of gender (read: the position of women) in Christian traditions, the article particularly explores two recent developments: first, the critical discussion in academic, theological and ecumenical circles of men and masculinities in contemporary Christian contexts, and second, the growing body of scholarship on the masculinity (or better, masculinities) of Jesus Christ in the New Testament in relation to masculinities in the early Christian era. Building on these debates and this scholarship, the article identifies a new and critical field of inquiry that explores the complex and productive relationships between the ambiguous and unstable masculinity/ies of Jesus Christ and the multiple and changing masculinities that are found today in the local contexts of an increasingly diverse global Christianity.
Effects of the combined artesunate and mefloquine antimalarial drugs on rat embryos
Authors: Boareto A.C. , Müller J.C. , Lourenço E.L.B. , Lombardi N. , Lourenço A.C. , Rabitto I. , de Morais R.N. , Rios F.S. , Dalsenter P.R. 
Published: February 2013

Artemisinins combination therapy (ACT) is the first choice therapy for falciparum malaria. Data on the safety of ACTs in pregnancy are limited and controversial and the use is not recommended on the first trimester. To evaluate the effects of isolated and combined artesunate (AS)/mefloquine (MQ) on embryo rats, pregnant rats were treated orally with AS (15 and 40 mg/kg body weight (bwt)/day), MQ (30 and 80 mg/kg bwt/day) and AS/MQ (15/30 and 40/80 mg/kg bwt/day) on days 9–11 post coitum (pc). The dams were euthanized on day 12 pc and gestational and embryos histological parameters were evaluated. Embryolethality and histopathological anomalies were significant when AS was given alone or combined with MQ. Combination of AS and MQ did not enhance their toxicity compared to their separate administrations; on the other side, there was a reduction in the toxic effects of the AS when combined with MQ. Isolated MQ did not induce developmental toxicity.
Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease
Authors: Le Huu Doanh , Matsushita Takashi , Jin Guihua , Hamaguchi Yasuhito , Hasegawa Minoru , Takehara Kazuhiko , Tedder Thomas F. , Fujimoto Manabu 
Published: February 2013

Key PointsCD19-deficient donors augmented Scl-cGVHD. Donor regulatory B cells suppressed Scl-cGVHD.
Systematic analysis of palatal transcriptome to identify cleft palate genes within TGFβ3-knockout mice alleles: RNA-Seq analysis of TGFβ3 Mice
Authors: Ozturk Ferhat , Li You , Zhu Xiujuan , Guda Chittibabu , Nawshad Ali 
Published: February 2013

Abstract

Background
In humans, cleft palate (CP) accounts for one of the largest number of birth defects with a complex genetic and environmental etiology. TGFβ3 has been established as an important regulator of palatal fusion in mice and it has been shown that TGFβ3-null mice exhibit CP without any other major deformities. However, the genes that regulate cellular decisions and molecular mechanisms maintained by the TGFβ3 pathway throughout palatogenesis are predominantly unexplored. Our objective in this study was to analyze global transcriptome changes within the palate during different gestational ages within TGFβ3 knockout mice to identify TGFβ3-associated genes previously unknown to be associated with the development of cleft palate. We used deep sequencing technology, RNA-Seq, to analyze the transcriptome of TGFβ3 knockout mice at crucial stages of palatogenesis, including palatal growth (E14.5), adhesion (E15.5), and fusion (E16.5).

Results
The overall transcriptome analysis of TGFβ3 wildtype mice (C57BL/6) reveals that almost 6000 genes were upregulated during the transition from E14.5 to E15.5 and more than 2000 were downregulated from E15.5 to E16.5. Using bioinformatics tools and databases, we identified the most comprehensive list of CP genes (n = 322) in which mutations cause CP either in humans or mice, and analyzed their expression patterns. The expression motifs of CP genes between TGFβ3+/− and TGFβ3−/− were not significantly different from each other, and the expression of the majority of CP genes remained unchanged from E14.5 to E16.5. Using these patterns, we identified 8 unique genes within TGFβ3−/− mice (Chrng, Foxc2, H19, Kcnj13, Lhx8, Meox2, Shh, and Six3), which may function as the primary contributors to the development of cleft palate in TGFβ3−/− mice. When the significantly altered CP genes were overlaid with TGFβ signaling, all of these genes followed the Smad-dependent pathway.

Conclusions
Our study represents the first analysis of the palatal transcriptome of the mouse, as well as TGFβ3 knockout mice, using deep sequencing methods. In this study, we characterized the critical regulation of palatal transcripts that may play key regulatory roles through crucial stages of palatal development. We identified potential causative CP genes in a TGFβ3 knockout model, which may lead to a better understanding of the genetic mechanisms of palatogenesis and provide novel potential targets for gene therapy approaches to treat cleft palate.
Eosinophilic granulomatous gastrointestinal and hepatic abscesses attributable to basidiobolomycosis and fasciolias: a simultaneous emergence in Iraqi Kurdistan
Authors: Hassan Hemmin A , Majid Runnak A , Rashid Nawshirwan G , Nuradeen Bryar E , Abdulkarim Qalandar H , Hawramy Tahir A , Rashid Rekawt M , Farris Alton B , Guarner Jeannette , Hughson Michael D 
Published: February 2013

Abstract

Background
Deep eosinophilic granulomatous abscesses, as distinguished from eosinophilic subcutaneous abscesses, are rare. Most reports are from the Far-East and India where the most commonly attributed cause is Toxocara. Sulaimaniyah in Northeastern Iraq has experienced an outbreak of eosinophilic granulomatous liver and gastrointestinal (GI) abscesses beginning in 2009. The purpose of this study was to determine the etiology and guide treatment.

Methods
The study was an ongoing investigation of patients having a histopathologic diagnosis of eosinophilic granulomatous abdominal abscesses in Sulaimaniyah hospitals from May 2009 to August 2012. Tissues were examined for organisms, and Enzyme Linked Immunoabsorbent Assays (ELISA) were performed for serum antibodies to Fasciola hepatica, Toxocara, and Echinococcus granulosus.

Results
Fourteen patients had granulomatous inflammation surrounding a central necrotizing eosinophilic exudate identified in surgical pathology specimens from abdominal surgeries. Two children and four adults had abscesses that formed GI masses. These patients included a 39 year old male with oropharyngeal and transverse colon disease, and a 48 year old male with liver and GI abscesses. All sites demonstrated a Zygomycete fungus surrounded by eosinophilic Splendori-Hoeppli material consistent with basidiobolomycosis. Five of the six patients with fungal disease were treated by surgery and 4 to 7 months of itraconozol. One child died of intestinal perforation while receiving IV amphotericin B; two adults required additional surgery for recurrent GI obstruction. Eight patients had isolated liver abscesses with no organisms identified by histopathology: ELISA results for F. hepatica were positive for five, borderline for one, and negative for two patients. These eight patients were treated for fascioliasis by surgical resection of localized abscesses and albendazol. One patient serologically positive for F. Hepatica was found to have a common duct fluke two years after initial diagnosis. Serological testing for Toxocara and Echinococcus granulosus was negative in all 14 patients.

Conclusions
Basidiobolomycosis and F. hepatica are implicated as the cause of abdominal eosinophilic granulomatous abscesses in 12 of 14 patients identified over a period of 40 months in northern Iraq. Treatment was complicated by chronic biliary tract disease in fascioliasis and perforation and recurrent intestinal obstruction with basidiobolomycosis.
A case-linkage study of crime victimisation in schizophrenia-spectrum disorders over a period of deinstitutionalisation
Authors: Short Tamsin B R , Thomas Stuart , Luebbers Stefan , Mullen Paul , Ogloff James R P 
Published: February 2013

Abstract

Background
Despite high rates of self-reported crime victimisation, no study to date has compared official victimisation records of people with severe mental illness with a random community sample. Accordingly, this study sought to determine whether persons with schizophrenia-spectrum disorders have higher rates of recorded victimisation than the general population, and to explore whether there have been changes in rates of recorded victimisation over a period of deinstitutionalisation.

Methods
The schizophrenia-spectrum cases were drawn from a state-wide public mental health register, comprising all persons first diagnosed with a schizophrenic illness in five year cohorts between 1975 – 2005. The criminal histories of 4,168 persons diagnosed with schizophrenic-spectrum disorders were compared to those of a randomly selected community sample of 4,641 individuals.

Results
Compared to community controls, patients with schizophrenia-spectrum disorders were significantly more likely to have a record of violent (10.1% vs. 6.6%, odds ratio 1.4) and sexually violent victimisation (1.7% vs. 0.3%, odds ratio 2.77), but less likely to have an official record of victimisation overall (28.7% vs. 39.1%, odds ratio 0.5). Over the approximate period of deinstitutionalisation, the rate of recorded victimisation has more than doubled in schizophrenia-spectrum patients, but stayed relatively constant in the general community.

Conclusions
People with schizophrenic-spectrum disorders are particularly vulnerable to violent crime victimisation; although co-morbid substance misuse and criminality both heighten the chances of victimisation, they cannot fully account for the increased rates. Deinstitutionalisation may have, in part, contributed to an unintended consequence of increasing rates of victimisation amongst the seriously mentally ill.
Investigation of rare variants in LRP1, KPNA1, ALS2CL and ZNF480 genes in schizophrenia patients reflects genetic heterogeneity of the disease
Authors: Jouan Loubna , Girard Simon L , Dobrzeniecka Sylvia , Ambalavanan Amirthagowri , Krebs Marie-Odile , Joober Ridha , Gauthier Julie , Dion Patrick A , Rouleau Guy A 
Published: February 2013

Abstract

Background
Schizophrenia is a severe psychiatric disease characterized by a high heritability and a complex genetic architecture. Recent reports based on exome sequencing analyses have highlighted a significant increase of potentially deleterious de novo mutations in different genes in individuals with schizophrenia.

Findings
This report presents the mutation screening results of four candidate genes for which such de novo mutations were previously reported (LRP1, KPNA1, ALS2CL and ZNF480). We have not identified any excess of rare variants in the additional SCZ cases we have screened.

Conclusions
This supports the notion that de novo mutations in these four genes are extremely rare in schizophrenia and further highlights the high degree of genetic heterogeneity of this disease.
Biochemical properties of a novel thermostable and highly xylose-tolerant β-xylosidase/α-arabinosidase from Thermotoga thermarum
Authors: Shi Hao , Li Xun , Gu Huaxiang , Zhang Yu , Huang Yingjuan , Wang Liangliang , Wang Fei 
Published: February 2013

Abstract

Background
β-Xylosidase is an important constituent of the hemicellulase system and it plays an important role in hydrolyzing xylooligosaccharides to xylose. Xylose, a useful monose, has been utilized in a wide range of applications such as food, light, chemical as well as energy industry. Therefore, the xylose-tolerant β-xylosidase with high specific activity for bioconversion of xylooligosaccharides has a great potential in the fields as above.

Results
A β-xylosidase gene (Tth xynB3) of 2,322 bp was cloned from the extremely thermophilic bacterium Thermotoga thermarum DSM 5069 that encodes a protein containing 774 amino acid residues, and was expressed in Escherichia coli BL21 (DE3). The phylogenetic trees of β-xylosidases were constructed using Neighbor-Joining (NJ) and Maximum-Parsimony (MP) methods. The phylogeny and amino acid analysis indicated that the Tth xynB3 β-xylosidase was a novel β-xylosidase of GH3. The optimal activity of the Tth xynB3 β-xylosidase was obtained at pH 6.0 and 95°C and was stable over a pH range of 5.0-7.5 and exhibited 2 h half-life at 85°C. The kinetic parameters K

m
and V

max
values for p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-L-arabinofuranoside were 0.27 mM and 223.3 U/mg, 0.21 mM and 75 U/mg, respectively. The k

cat
/K

m
values for p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-L-arabinofuranoside were 1,173.4 mM-1 s-1 and 505.9 mM-1 s-1, respectively. It displayed high tolerance to xylose, with K

i
value approximately 1000 mM. It was stimulated by xylose at higher concentration up to 500 mM, above which the enzyme activity of Tth xynB3 β-xylosidase was gradually decreased. However, it still remained approximately 50% of its original activity even if the concentration of xylose was as high as 1000 mM. It was also discovered that the Tth xynB3 β-xylosidase exhibited a high hydrolytic activity on xylooligosaccharides. When 5% substrate was incubated with 0.3 U Tth xynB3 β-xylosidase in 200 μL reaction system for 3 h, almost all the substrate was biodegraded into xylose.

Conclusions
The article provides a useful and novel β-xylosidase displaying extraordinary and desirable properties: high xylose tolerance and catalytic activity at temperatures above 75°C, thermally stable and excellent hydrolytic activity on xylooligosaccharides.