bluuebunny/crossref_metadata_2025_split huggingface dataset:
The chromatin accessibility and transcriptomic landscape of the aging mice cochlea and the identification of potential functional super-enhancers in age-related hearing loss
Authors: Zhang Chanyuan , Yang Ting , Luo Xiaoqin , Zhou Xiaoqing , Feng Menglong , Yuan Wei 
Published: July 2024

AbstractBackgroundPresbycusis, also referred to as age-related hearing loss (ARHL), is a condition that results from the cumulative effects of aging on an individual's auditory capabilities. Given the limited understanding of epigenetic mechanisms in ARHL, our research focuses on alterations in chromatin-accessible regions.MethodsWe employed assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) in conjunction with unique identifier (UID) mRNA-seq between young and aging cochleae, and conducted integrated analysis as well as motif/TF-gene prediction. Additionally, the essential role of super-enhancers (SEs) in the development of ARHL was identified by comparative analysis to previous research. Meanwhile, an ARHL mouse model and an aging mimic hair cell (HC) model were established with a comprehensive identification of senescence phenotypes to access the role of SEs in ARHL progression.ResultsThe control cochlear tissue exhibited greater chromatin accessibility than cochlear tissue affected by ARHL. Furthermore, the levels of histone 3 lysine 27 acetylation were significantly depressed in both aging cochlea and aging mimic HEI-OC1 cells, highlighting the essential role of SEs in the development of ARHL. The potential senescence-associated super-enhancers (SASEs) of ARHL were identified, most of which exhibited decreased chromatin accessibility. The majority of genes related to the SASEs showed obvious decreases in mRNA expression level in aging HCs and was noticeably altered following treatment with JQ1 (a commonly used SE inhibitor).ConclusionThe chromatin accessibility in control cochlear tissue was higher than that in cochlear tissue affected by ARHL. Potential SEs involved in ARHL were identified, which might provide a basis for future therapeutics targeting SASEs related to ARHL.
Analysis of events from sudden isolated dysarthria to diagnosis of myasthenic crisis: myasthenia gravis mimicking acute lacunar stroke—a case report
Authors: Miletic Simona Petkovic , Ahmed Sheikh Ruksana Binte 
Published: July 2024

Abstract
Background
Myasthenic crisis (MC) is a life-threatening complication of myasthenia gravis (MG), necessitating ventilation. Achieving a safe and timely diagnosis of myasthenic crisis with atypical, isolated presentation is a considerable challenge particularly in elderly patients, where myasthenia gravis can present with isolated dysarthria in rare instances, giving a clinical impression of lacunar stroke.

Case presentation
We present a compelling case of a 73-year-old Caucasian female presenting with abrupt onset of isolated dysarthria. Despite initial treatment for a presumed lacunar stroke, subsequent evaluations led to her diagnosis of a myasthenic crisis. Within 72 h of admission, the patient developed dysphagia and shortness of breath, requiring supplemental oxygen. The case highlights the sequential progression of events from the atypical presentation of isolated dysarthria and its course to the management of a myasthenic crisis.

Conclusion
Our reported case focuses on the discussion of myasthenia that mimicked a lacunar stroke and was finally diagnosed at a critical time of medical crisis. This case highlights the imperative notion that isolated dysarthria in elderly individuals warrants vigilant monitoring for possible myasthenia gravis, given the low incidence of lacunar stroke presenting with only dysarthria.
Decoding the role of DNA methylation in allergic diseases: from pathogenesis to therapy
Authors: Han Ruiming , Zhu Dongdong , Sha Jichao , Zhao Boning , Jin Peng , Meng Cuida 
Published: July 2024

AbstractAllergic diseases, characterized by a broad spectrum of clinical manifestations and symptoms, encompass a significant category of IgE-mediated atopic disorders, including asthma, allergic rhinitis, atopic dermatitis, and food allergies. These complex conditions arise from the intricate interplay between genetic and environmental factors and are known to contribute to socioeconomic burdens globally. Recent advancements in the study of allergic diseases have illuminated the crucial role of DNA methylation (DNAm) in their pathogenesis. This review explores the factors influencing DNAm in allergic diseases and delves into their mechanisms, offering valuable perspectives for clinicians. Understanding these epigenetic modifications aims to lay the groundwork for improved early prevention strategies. Moreover, our analysis of DNAm mechanisms in these conditions seeks to enhance diagnostic and therapeutic approaches, paving the way for more effective management of allergic diseases in the future.
Anti-thrombotic effects of arteanoflavone by regulating cyclic nucleotides and aggregation on human platelets
Authors: Choi Ho Keun , Kim Ga Yeon , Lee Ga Hee , su Jang Hee , Kang Da Hyeon , Lee Jin Pyo , Lee Dong-Ha 
Published: July 2024

AbstractExcessive clotting or abnormal platelet accumulation can lead to serious cardiovascular disorders such as atherosclerosis, stroke, and thrombosis. Therefore, it is imperative to identify compounds capable of controlling or impeding platelet aggregation to prevent the onset of cardiovascular diseases. Arteanoflavone, a compound extracted from Artemisia iwayomogi, has not garnered scientific recognition for its potential health benefits, recent studies have substantiated its anti-inflammatory, antioxidant, and anti-allergic properties. However, the precise mechanisms by which arteanoflavone influences platelet aggregation and blood clot formation have not been conclusively established. This research investigates arteanoflavone’s role in these processes, particularly in platelets induced by collagen. The study reveals a significant increase in the production of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) correlating with the administered dosage of arteanoflavone. Concurrently, a noticeable escalation is observed in substrates of cAMP-dependent kinase and cGMP-dependent kinase, specifically VASP and inositol 1,4,5-trisphosphate receptor (IP3R).Arteanoflavone demonstrates its ability to limit Ca2+ movement in the dense tubular system through IP3R phosphorylation. Moreover, phosphorylated VASP inhibits the binding of fibrinogen to αIIb/β3, thus suppressing platelet activity. Arteanoflavone also stimulates the phosphorylation of PI3K/Akt, a protein linked to platelet granule release, and MAPK (ERK, JNK, and p38) protein, associated with both platelet granule release and TXA2 production.Lastly, arteanoflavone impedes collagen-induced platelet aggregation and blood clot formation by inhibiting fibrin production in thrombin-induced platelets. Hence, it is suggested that arteanoflavone could be valuable as an agent that effectively deters platelet inhibition and blood clot formation through antiplatelet mechanisms.
Are university students really hungry for sustainability? A choice experiment on new food products from circular economy
Authors: di Santo Naomi , Califano Giovanbattista , Sisto Roberta , Caracciolo Francesco , Pilone Vittoria 
Published: July 2024

AbstractConsumers are demonstrating increasing awareness towards initiatives promoting sustainable practices in the food sector. This is particularly pronounced among university students, a segment known for its receptivity to sustainability initiatives. Utilizing a choice experiment, this study examines the Italian students’ preferences for fruit juice produced by a hypothetical company. Specifically, the research explicitly assesses the premium price attributable to a circular economy certification—a relatively new standard—in comparison with the widely recognized and appreciated organic certification. Furthermore, the study evaluates the potential additive value of possessing both certifications jointly. Results indicate that consumers are willing to pay a premium for products labelled with circular economy attributes. However, in the presence of an organic certification, the additive value of the circular economy certification is diminished by more than half. Finally, consumers who have received environmental training demonstrate a higher premium for such products, highlighting the role of education in promoting sustainable choices. These findings suggest that a potential market exists for circular economy-certified products, providing an incentive for businesses to adopt circular economy practices. Environmental training plays a relevant role in fostering sustainable transitions, thereby underscoring the significance of education in shaping conscious consumer behaviour.
Water-holding properties and electrochemical characterization of the soil under the Grand Deliverance Hall of the Chongshan Temple, Taiyuan City, China: providing the basis for preventing groundwater erosion in historic buildings
Authors: Lu Yi , Bai Xiangling , Wang Xiaolong , Han Lin , Bai Xiaojian , Han Pengju , An Yijing 
Published: July 2024

Abstract
Many historic buildings are at serious risk of deterioration due to changes in the moisture content of the soil under the buildings. Based on the typical deterioration problems of the Grand Deliverance Hall and the cultural relics in the hall, this study analyzes and concludes that the main factor affecting the generation and development of deterioration is groundwater erosion through environmental monitoring and geotechnical engineering investigation. The impact of changes in moisture content on the water-holding properties and pore distribution of the soil under the cultural-relics buildings was further assessed. Then, combined with the soil property, our study provides a novel and effective way to monitor and reflect the water-holding properties of the soil using electrochemical detection. The connected pore resistance and matrix suction decrease with the increase in the moisture content, both showing a power function law. Changes in moisture content cause changes in the thickness of the double electric layer, which in turn affects the connected pore resistance and matrix suction of soil. The greater the connected pore resistance, the greater the matrix suction and the water holding capacity. Finally, based on the research of this study and the conservation ideology of cultural relics that respects the original and minimizes intervention, protection recommendations to prevent groundwater erosion are proposed in order to provide guidance for future research.

Graphical Abstract
‘The Listening Series’: increasing equity, diversity and inclusion in patient and public involvement and engagement for policy research by listening to and learning from under-represented groups
Authors: Bevan Charlotte , Alderdice Fiona , Darby Sally , Gilzean-Hughes Serena , McLeish Jenny , Mulla Sumayya , Plachcinski Rachel , Wilkinson Sophia , Williams Harriet , Rowe Rachel 
Published: July 2024

Abstract
Background
Policy research aims to provide evidence to inform government policy decisions about health and social care. Engaging and involving the public and patients in this work is widely recognised as essential. Research funders prioritise equality, diversity and inclusion (EDI) in patient and public involvement and engagement (PPIE), but people who are most likely to experience poor outcomes are also those least likely to be involved in research. This paper describes our experience of setting out to understand how to overcome barriers to EDI in PPIE in the research carried out by the National Institute for Health and Care Research (NIHR) Policy Research Unit in Maternal and Neonatal Health and Care (PRU-MNHC), in a PPIE consultation project we called The Listening Series.

Methods
We convened five video-recorded online discussion groups involving 20 individuals advocating for groups who are under-represented in our research. Those taking part included people working with Black and Asian women and families, young parents, those from socially deprived backgrounds, and women and families with physical and learning disabilities. Discussions focussed on practical solutions to addressing challenges to people being excluded, and how to improve EDI in our research.

Learning and reflection
Five key themes were identified: ‘build trust’; ‘involve us from the beginning’; ‘show us impact’; ‘use clear, appropriate and inclusive communication’; and ‘imagine life in our shoes’. We used the learning to create a guidance document for researchers and an accompanying 15-minute film. We also took practical steps to embed the learning strategically by expanding our Task Group for PPIE in the PRU-MNHC to include four Listening Series invitees with a remit to champion EDI in our research and ensure that it is embedded in our PPIE activities. We continue to reflect on and work to address the associated challenges.

Conclusions
The Listening Series helped us rethink our processes for inclusion to go beyond traditional methods of involvement and engagement. The themes identified pose challenges that require time, resource and empathic engagement from researchers to be meaningfully resolved. This has implications for policy makers and research funders who need to consider this in their processes.
Uncovering a hidden danger: a case report of diffuse coronary spasm concealing spontaneous coronary artery dissection
Authors: Desario Paolo , Rutigliano David , Palumbo Vincenzo , Caldarola Pasquale 
Published: July 2024

Abstract
Background
Over recent years, spontaneous coronary artery dissection (SCAD) has emerged as a no longer rare cause of acute coronary syndrome (ACS). On the other hand, coronary artery spasm (CAS) is the main cause of ischemic heart disease with non-obstructive coronary lesions. Clinical manifestations of both vary from stable angina to ACS or, rarely, sudden cardiac death. These entities may be underdiagnosed on a coronary angiography.

Case presentation
We report the case of a young woman presenting with acute chest pain and no coronary risk factors. Angiography revealed a focal subcritical stenosis of the right coronary artery. Coronary wiring resulted in diffuse and critical spasm. However, optical coherence tomography (OCT) and intravascular ultrasound (IVUS) showed extensive SCAD. She was therefore treated conservatively. On the fourth day, cardiac computed tomography angiography (CCTA) excluded disease progression, and then she was discharged on medical therapy.

Conclusions
Combined IVI plays a vital role in providing accurate and detailed visualization of the coronary anatomy and thus allowing for more precise diagnosis, risk stratification, and treatment planning. CCTA can be considered a valuable tool in the noninvasive follow-up of SCAD.
From theory to practice in implementation science: qualitative insights from the implementation model developed by a commercial eMental Health provider
Authors: Bastoni Sofia , van Lotringen Charlotte Marijne , Kip Hanneke , Sanderman Robbert , van Gemert-Pijnen Lisette J. E. W. C. , van Dongen Anne 
Published: July 2024

Abstract
Background
Although eMental health interventions are a viable solution to address disparities in access to mental healthcare and increase its efficiency, they still face challenges of implementation. Literature highlights numerous barriers such as diffusion of responsibility and unclear expectations of what implementation entails might hinder this process. While research mostly focuses on analyzing these barriers, there is an urgent need to increase uptake in practice. In turn, commercial companies focus mostly on increasing uptake, while overlooking research outputs. To bridge the gap between research and practice, attention to how implementation occurs in practice is required. This study investigates “Make it Happen” (MiH), the implementation model developed by the eMental Health company Minddistrict, aiming to gain more insight into operationalizing implementation frameworks by 1) describing MiH and its conceptual underpinnings, and 2) gaining lessons learned from the development of MiH. Ultimately, this work aims at improving existing scientific frameworks by extending them with knowledge from practice.

Methods
First, individual interviews and focus groups with Minddistrict implementation managers were performed. Second, individual interviews with project leads in mental healthcare organizations that were involved in the implementation of Minddistrict were conducted. Within Minddistrict, 7 implementation managers and account managers were involved, in addition to 11 project leads from mental healthcare organizations. Data were elaborated with thematic analysis.

Results
A comprehensive description of MiH and its 5 main phases was achieved. During the 1) Onboarding phase, implementing organizations are guided by Minddistrict to build a team responsible for implementation, which then 2) designs patient and client journeys, 3) builds, tailors and configures their offer, 4) trains key-users and, 5) evaluates the success of implementation. All participants had extensive and aligned definitions and articulated expectations on implementation. Points of improvement for the model such as role ambiguity and excessive workload were identified. As strengths, internal motivation and good relationships with the provider were valued.

Conclusion
The present study highlights the importance of clear role division and stakeholder engagement in implementation processes, and suggest that a strong collaboration between companies and academia could optimize implementation efforts and ensure a better fit between humans, context, and technologies.
Botulinum toxin in management of back pain
Authors: Łuczak Blanka , Nowińska Bianka , Słychan Katarzyna , Hądzlik Izabela , Piotrowski Jan , Biały-Karbowniczek Julia , Bulska Klaudia , Brzozowska Patrycja , Piela Andrzej , Sławek Konrad 
Published: July 2024

Introduction
The botulinum toxin is a widely used therapeutic agent. It is used for aesthetic reasons in treatment of glabellar wrinkles. Therapeutic indications include cervical dystonia, hyperhidrosis or migrenes. Also, it can be used in pain management, especially back pain. Back pain is one of the most common pain syndromes decreasing quality of life. Treatment involves various methods such as conservative and surgical approach. One of the methods is botulinum toxin.
Objectives
This article aims to evaluate usage, mechanism and treatment effects of back pain using botulinum toxin.
Methods
A Literature review of articles published in Pubmed between 2000 and 2024 using following words “botulinum toxin”, “botulinum”, “back pain” and “treatment”.
Results
Botulinum toxin injections are one of the pain management methods used especially in low back pain and myofascial pain syndrome. Treatment relies on its myorelaxant properties and inhibition of neuromodulator secretion such as substance P or acetylocholine. Pain relief effects are noted after three weeks and can last up to six months. In the initial stages a significant amount of patients report relief of pain.
Conclusion
Botulinum toxin is one of the treatment methods for lower back pain. It is a safe, minimally invasive and effective treatment. Patients can feel the relief even in three weeks and the effects might persist even up to six months.
A case of Legionella pneumonia with rhabdomyolysis, with extremely high creatinine kinase without acute kidney injury in an adult
Authors: Sangam Sangam , Malik Sehrish , Hodo Franc , Patel Bhawesh , Bengualid Victoria , Sharma Shorabh 
Published: July 2024

Legionella pneumophila is a bacterium that usually causes pulmonary disease but can rarely present with extrapulmonary manifestations, such as rhabdomyolysis. This is a case of Legionella infection with significant rhabdomyolysis but a lack of acute kidney injury. A 38-year-old male with a history of epilepsy presented to the emergency department after a seizure episode with confusion, fever, emesis and bruises. He also complained of a productive cough and scant haemoptysis for the past two months. Chest X-ray showed retrocardiac and left upper lobe opacities; urine was positive for Legionella antigen and myoglobinuria. Creatinine phosphokinase was 242,488 U/l and creatinine was 0.5 mg/dl. The patient was managed with oxygen therapy, aggressive IV hydration and IV azithromycin, and later IV levofloxacin until his symptoms resolved. Rhabdomyolysis may be a sign of Legionella infection. Rapid testing of Legionella antigen, especially in populations at risk, may be crucial for timely diagnosis and treatment. Kidney function may be preserved in the early stages of disease, but early treatment with antibiotics and aggressive hydration are an effective way to prevent deterioration in kidney function.
Discharging older patients from hospital to homecare: conflicts in collaborative practices among nurses across sectors
Authors: Hansen Mette Frier , Martinsen Bente , Galvin Kathleen , Norlyk Annelise 
Published: July 2024

Background: Collaboration is a key factor influencing the quality and safety in patients transition between sectors. However, specific collaborative practices may give rise to conflict between hospital nurses and community nurses. Aims: To gain a deeper understanding of collaborative practices which have the potential to fuel tension in collaboration between hospital nurses and community nurses during discharge of older patients from hospital to homecare. Methods: A meta-ethnography approach was used in this study and a systematic literature search was conducted in 2022. Results: Five themes were identified in the analysis. These themes revealed how uncertainty, limited confidence in information and personal attitude in communication may fuel tension between hospital nurses and community nurses. Tensions arising from a negative loop emerged because of uncertainty, causing a growing rift between hospital nurses and community nurses, leaving them as opponents rather than collaborators. The authors suggest that policy makers and managers can break this loop by underpinning shared policies and awareness of common objectives.
Finishing and detailing with the pre-adjusted edgewise labial fixed appliance: the why, the what and the how
Authors: Donovan Joey , McMorrow Siobhan , Salloum Erfan , Millett Declan T , McIntyre Grant T 
Published: July 2024

Finishing and detailing is typically undertaken towards the end of orthodontic treatment. It should, however, be considered at the start of treatment and then evaluated at the end of each treatment stage. An appraisal of the disharmony between the planned and observed facial, skeletal and occlusal objectives is required with ongoing refinement to achieve the treatment goals. With the pre-adjusted edgewise labial fixed appliance, finishing and detailing may involve bracket repositioning, archform adjustment, localized wire bending, elastic traction, partial debonding, occlusal adjustment as well as restorative and periodontal input. It should also take into consideration the retention plan. CPD/Clinical Relevance: This article summarizes what is involved in finishing and detailing with the pre-adjusted edgewise labial fixed appliance in regard to aesthetics, function and stability, and critiques the evidence available in relation to these aspects.