bluuebunny/crossref_metadata_2025_split huggingface dataset:
VERRUCIFORM XANTHOMA OF THE SCROTUM
Authors: Shindo Yasuko , Mikoshiba Hajime , Okamoto Kikuhiko , Morohashi Masaaki 
Published: April 2015

ABSTRACTWe report two cases of verruciform xanthoma of the scrotal skin. In each case, the tumor was a single pink lesion with firm consistency and a granular surface. Each lesion was characterized histopathologically by papillomatous proliferation of the epidermis and dermis and dense foam‐cell infiltration of the region corresponding to the dermal papillary layer. These cells proved to be xanthoma cells that stained negatively or weakly positively with PAS, colloidal iron, and Alcian blue and strongly positively with Sudan III. No medical problems were found in either patient, and serum lipid levels were normal. This report also presents electron microscopic findings from scrotal verruciform xanthoma in one case and the results of lipid analysis of tumor tissue from both cases.
SUCCESSFUL TREATMENT OF ULCERATED NECROBIOSIS
Authors: Sawada Yukimasa 
Published: April 2015

ABSTRACTA case of a 58‐year‐old man with ulcerated necrobiosis lipoidica diabeticorum on the lateral aspect of the left foot was described. The ulcer showed no tendency toward healing despite conservative treatment and simple closure. However, a stable covering was achieved by skin flap transfer after radical debridement following intravenous prostaglandin E1 infusion.
PSEUDOANEURYSM AS A COMPLICATION OF ANGIOGRAPHY
Authors: Kikuchi Ichiro , Inoue Shouhei , Sakaguchi Ei 
Published: April 2015

ABSTRACTA 10 times 20mm nonpulsating cyst‐like swelling appeared at the angiography site on the right inguinal region of a 60‐year‐old man; the swelling was biopsized 89 days after angiography. Infrared thermography performed prior to biospy revealed a hypothermic area within a larger hyperthermic area, suggesting that this was a pseudoaneurysm. Histological examination suggested pseudo‐pyogenic granuloma.
Hyperlipoproteinaemia and Xanthomatosis as a Model of Cholesterol Ester Accumulation
Authors: Vermeer Bert Jan 
Published: April 2015

AbstractA local event which occurs after damage of a blood vessel can be the start of cellular cholesterol ester accumulation. Thrombocytes and macrophages will adhere to the endothelial wall. Subsequently lipoproteins and macrophages permeate this endothelial wall. The perivascularly arranged macrophages will internalize the “modified lipoproteins” and eventually cholesterol ester accumulation will take place. This cholesterol ester accumulation can also occur in smooth muscle cells. The reversibility of the accumulation depends on the lipoprotein levels and on the physico‐chemical characteristics of the accumulated cholesterol (esters). This phenomenon takes place in the formation of xanthomas as well as in atherogenesis.
Comparative Reactivity of a Panel of Monoclonal Antibodies on Yucatan Mini‐Pig and Human Skin
Authors: Schmitt Daniel , Gracia Anne‐Marie , Viac Jocqueline , Ohrt Christiane , Thivolet Jean 
Published: April 2015

AbstractYucatan mini‐pig skin (Mexican hairless pig) is described as a good animal model for comparative investigations with human skin. We report the reactivity patterns of monoclonal antibodies (MCA) on both mini‐pig and human skin. Of 21 MCA reacting with various human skin antigens, only six were negative with mini‐pig skin.Using indirect immunofluorescence test, MCA specific for cytoplasmic or membrane antigens of human keratinocytes (glycoproteins, cytokeratins, desmosomes) gave the same reactivity patterns on mini‐pig skin in the epidermis.In the dermis and at the dermal‐epidermal junction, most MCA decorated the same structures on both substrates (laminin, oxytalan fibers, neurofilaments, fibroblasts). However, some of them did not stain the same cellular structures.As expected, MCA specific for HLA antigens did not show any reactivity with mini‐pig skin.These MCA offer new tools for comparative investigations in various fields of experimental dermatology.
Combination Therapy of Oral Methoxypsoralen: Photochemotherapy (PUVA) and an Aromatic Retinoid (Etretinate, Tigason) in the Treatment of Psoriasis
Authors: Thirumoorthy Thamotharampillai , Tham Siew Nee , Tan Yoke Choo 
Published: April 2015

AbstractRePUVA is the combination of an oral aromatic retinoid, etretinate, with oral photochemotherapy, PUVA. This combination therapy has the advantage of faster clearance with fewer side effects.In Middle Road Hospital, we treated 29 patients with extensive psoriasis (more than 30% involvement) with a total 32 RePUVA treatments. There was complete clearance in 69% of patients with an average of 19 irradiations and an average total ultraviolet A (UVA) dose of 183 J/cm2. This compared well with standard PUVA which needed 25 radiations and a total UVA dose of 346 J/cm2.The main side effects were cheilitis, exfoliation of skin and pruritus. Biochemically, 41% of the patients showed elevations of serum triglyceride.
A Tumor with Sebaceous Differentiation Showing Intraepidermal Epithelioma
Authors: Aso Miki , Shimao Shuhei 
Published: April 2015

AbstractA sixty‐year‐old woman had a small papule within a plaque on the left arm. Histologically, the papule was similar to irritated seborrheic keratosis or inverted follicular keratosis and the plaque was intraepidermal epithelioma. However, both lesions included mature sebaceous cells showing sebaceous differentiation. This tumor may be closely related to the pilo‐sebaceous unit or sebaceous gland. To our knowledge, no similar tumor has been reported in the literature.
Cutaneous Melanoma
Authors: Shibata Henry Ryusuke 
Published: April 2015

AbstractCutaneous melanoma is an enigmatic cancer with good survival rates with management at an early stage, i.e. thin superficial lesions localized to the primary site. However, once it becomes deeply invasive with evidence of metastasis, it is one of the most aggressive and lethal cancers. Many investigations have been done and are being conducted in the areas of prevention, (identification and removal of precursor lesions, sun avoidance and use of sun screen preparations), diagnosis (monoclonal antibodies, tumour markers) and therapy (immunotherapy, hyperthermic perfusion and randomized clinical trials. From the Royal Victoria Hospital, Montreal, Quebec, personal experiences with the use of a Levamisole/Placebo — double blind randomized clinical trial for Stage I Melanoma patients is presented. The results of another trial of combination chemoimmunotherapy utilizing BCG/DTIC and/or CCNU for Stage II patients is discussed. Experimental studies into the lymphocytic reaction of lymph nodes draining a primary melanoma site have been initiated and changes in the number of T cells and B cells in the lymph nodes have been noted, reflecting some systemic changes in the host. A prospective randomized surgical protocal on cutaneous melanoma of intermediate thickness (1 to 4 mm) is underway to determine whether a conservative local excision is sufficient and whether involved regional nodes are an indicator and not an instigator of further disease (Intergroup Melanoma Study). Hyperthermic limb perfusion as a therapeutic modality has still to be proven as a useful procedure in the management of melanoma. A clinical trial is urgently required. Increasing public and professional awareness coupled with pertinent clinical and basic research into various aspects of this disease make the future of the patient with melanoma not as black as it seems!
Immunohistochemical Study of HLA Antigens and Lymphocyte Infiltration in Melanocytic Tumors
Authors: Kageshita Toshiro , Nagano Hiroaki , Johno Masayoshi , Ono Tomomichi , Arao Tatsuyoshi , Imai Kohzoh 
Published: April 2015

AbstractUsing monoclonal antibodies, we have examined immunohistochemically the expression of HLA‐heavy chain, beta‐2 microglobulin and HLA‐DR antigen in benign and malignant melanocytic tumors. In malignant melanoma, these antigens were expressed on the cell membrane, but, in pigmented and blue nevi, these antigens were decreased or absent. In particular, the expression of HLA‐DR was restricted to the cell membrane of some malignant melanomas, so its expression on a melanocytic cell lineage might be a useful diagnostic marker. Furthermore, we studied the degree of lymphocytic infiltration into melanotic tumors, using the expression of HLA antigens as the marker. Our results did not indicate any significant relationship between HLA‐DR positive melanoma and lymphocyte infiltration.
Supplement of Exogenous IL2 Abrogates Induction of Tolerance in Drug‐Induced T Cell Proliferation
Authors: Ikezawa Zenro , Kawaguchi Hiroshi 
Published: April 2015

AbstractAn intravenous injection of large doses of semi‐synthetic penicillin, sulbenicillin (SBPC) 7 days before immunization induced immunological tolerance of lymphocyte proliferative response to SBPC. This nonresponsiveness of the tolerant lymph node cells to SBPC was not changed into responsiveness by removing the Lyt‐2+ suppressor type T (Ts) subset. However, an in vivo interleukin 2 (IL2) treatment changed the nonresponsiveness of the tolerant Lyt‐1+2– helper type T (Th) cells into responsiveness. These results suggest that the nonresponsiveness of the tolerant lymph node cells is due to clonal anergy or deletion of the Lyt‐1+2– Th cells rather than to involvement of Lyt‐2+ Ts cells, and support the hypothesis that tolerance is a consequence of IL2 deficit during the T cell recognition of antigen.
Kimura's Disease: Immunohistochemistry of Infiltrating Cells Using Monoclonal and Polyclonal Antibodies
Authors: Maeda Kazuo , Jimbow Kowichi 
Published: April 2015

AbstractThis study reports immunohistochemical findings with polyclonal and monoclonal antibodies in a case of Kimura's disease, angiolymphoid hyperplasia with eosinophilia, which showed a histopathology similar to that of follicular lymphoma in the initial biopsy. Major findings in our case were the variations in distribution of eosinophils, mast cells and IgE producing plasma cells between the biopsies. No proliferation of eosinophils was noted in the initial biopsy. The follicular structures in Kimura's disease appeared immunohistochemically to have derived from a reactive inflammatory process. It is suggested that the frequency of eosinophils in Kimura's disease varies with the course of the disease and that there is no direct relationship between the distributions of mast cells and eosinophils.
Five Cases of Porphyria Cutanea Tarda with Mild Cutaneous Changes: Evaluation of the Efficacy of Phlebotomy by the Pattern Analysis of Urinary Porphyrins
Authors: Nonaka Shigeo , Ohgami Taro , Murayama Fumio , Yamashita Kazunori , Yoshida Hikotaro 
Published: April 2015

AbstractFive cases of porphyria cutanea tarda (PCT) with mild cutaneous changes are reported. Acute episodes of photosensitivity were not seen in all patients. Laboratory examinations showed a predominance of uroporphyrin (UP) excretion in the urine in 4 out of 5 cases. One case showed a relatively low urinary porphyrin level even though abnormality of the porphyrin pattern was present. Four cases showing a predominance of UP excretion in the urine revealed a high level of serum coproporphyrin (CP) as well. Histopathologically, all cases showed a deposition of PAS positive materials surrounding the small blood vessels in the upper dermis, in addition to the deposition of IgG materials visualized by the immunofluorescent technique.Phlebotomy was performed fifteen times in one case. The urinary porphyrin level was gradually reduced to normal levels and the urinary porphyrin pattern shifted from a predominance of UP and hepta‐carboxyl porphyrin to a predominance of CP excretion. Analysis of the urinary porphyrin profile is useful as a laboratory tool for diagnosis or observation of the course of PCT, as well as for the quantitative analysis of urinary porphyrins.
Bullous Lichen Planus Developed on Erythema Ab Igne
Authors: Horio Takeshi , Imamura Sadao 
Published: April 2015

AbstractLichen planus developed on erythema ab igne which was induced by repeated exposures to a stove. The clinical and histopathological features of the eruption were an admixture of typical lichen planus and erythema ab igne. Subepidermal bullae were also found on the lichen planus. Direct immunofluorescence tests revealed deposits of fibrin in the basement membrane zone and IgM in the colloid bodies. Indirect immunofluorescence studies detected no circulating antibodies against cutaneous structures in the patient's serum. This study indicates that repeated thermal exposure over a prolonged period may produce lichen planus lesions.
A Case of Extensive Subcutaneous Sarcoidosis
Authors: Yamada Saori , Yagi Ayako , Shiraishi Satoshi , Miki Yoshiharu 
Published: April 2015

AbstractA 61‐year‐old female with extensive subcutaneous epithelioid cell granuloma in the back and extremities had negative tuberculin reaction, positive anti‐nuclear factor (ANF) and elevated serum angiotensin‐converting enzyme (ACE) level while on oral clofibrate for one‐and‐a‐half years because of hyperlipemia. She also suffered from ischemic heart disease with pericardial effusion and dependent edema. The skin surface temperatures over the lesions were about 2°C higher than those of the surrounding normal skin. The subcutaneous lesions cleared spontaneously within 4 months after clofibrate was discontinued. Tuberculin reaction became positive and serum ACE returned to the normal level.
An Acantholytic Variant of Seborrheic Keratosis
Authors: Uchiyama Noriko , Shindo Yasuko 
Published: April 2015

AbstractA case of an acantholytic seborrheic keratosis, first reported by Tagami and Yamada, was seen in a 63‐year‐old woman. It appeared to belong to the irritated type of Lever's classification.
Induction of Pemphigoid Lesions in Neonatal Mice
Authors: Mitsuhashi Yoshihiko , Takahashi Masa‐aki , Kiuchi Hiyoshi , Mikami Hideki , Hashimoto Isao , Katabira Yasuo 
Published: April 2015

AbstractIn order to study the pathogenetic role of anti‐basement membrane zone antibodies (BMZ‐Ab) in bullous pemphigoid (BP), parenteral transfer of serum, heat inactivated serum, blister fluid, or IgG from three patients with BP into neonatal mice was carried out. BP‐like lesions developed in 6 of 8 mice receiving the serum from one of the patients. However, no lesions were observed in 100 other mice that were given serum, blister fluid or IgG from the other two patients or three healthy controls. Analysis of the characteristics of these injected materials revealed that, for induction of the BP‐like lesions in neonatal mice, it is necessary that both complement‐fixing BMZ‐Ab and components of complement are present. The role of inflammatory cells in the pathogenesis of BP could not be determined by the present experiments.
Increased C5a Anaphylatoxin in the Sera of Psoriatic Patients and Patients with Inflammatory Dermatoses
Authors: Ohkohchi Kyoko , Takematsu Hideaki , Tagami Hachiro 
Published: April 2015

AbstractWe measured C5a and C5a des arg (collectively called C5a) by radioimmunoassay in the sera of psoriasis and other widespread inflammatory dermatoses. Larger amounts of serum C5a were detected in patients with psoriasis and other inflammatory dermatoses than in normal healthy controls. In the psoriatic patients, the serum C5a concentrations did not correlate with extent or with activity of the skin lesions. However, when followed in 6 psoriatic patients, the C5a levels showed changes in parallel with the skin lesions. Our results suggest that complement activation with resultant release of C5a anaphylatoxin takes place in psoriasis as well as in other widespread inflammatory skin disorders.
Case of Epidermodysplasia Verruciformis with a 12‐Year‐Follow‐Up
Authors: Sait Mohammed Ameen , Garg Bhagat Ram 
Published: April 2015

AbstractAn interesting case of epidermodysplasia verruciformis with a 12‐year‐follow‐up is reported. During the period of observation, the patient developed leprosy (TT), skin tuberculosis, molluscum contagiosum, tinea corporis and condylomata acuminata lesions. Mantoux, lepromin and DNCB sensitization tests for cell mediated immunity (CMI) were negative. The significance of these findings is briefly discussed.
The Role of MCA in Dermatology: Monoclonal Antibodies in Cutaneous Immunohistochemistry
Authors: Thivolet Jean 
Published: April 2015

AbstractSince the first demonstration of antibodies in sera of pemphigus patients by immunofluorescence in 1964, dermatology has become one of the leading clinical specialities applying immunohistochemical methods for both research and current diagnosis. The first step has been the study of Ig deposits in autoimmune or other immunologically related skin diseases such as, for example, pemphigus, pemphigoid, D.H. and L.E.In fact, the diagnosis of autoimmune diseases is no longer the only field of immunohistochemistry. More recently, IHC has been applied to a new field, the study of skin components, cell surface antigens, molecules, and fibers such as collagen, keratin, and cellular cyto‐skeleton (1).The indicators for studying skin antigens are the corresponding antibodies. Autoantibodies reactive with the epidermis have facilitated the study of skin structures in normal as well as in diseased skin. Nevertheless, the field of such studies remained limited because few antisera were available. In a large majority of cases, antigens were unknown and nonbiochemically studied. Therefore, attempts have been made to produce heterologous conventional antisera against various components of the skin. For example, in 1980 (2) J. Viac in our group produced antikeratin antisera by immunizing guinea pig and rabbits with either whole keratin or polypeptidic bands isolated on polyacrylamid gels. Such antibodies recognized different antigenic species of keratins and provided a good method for studying normal epithelia and tumors. Conventional polyclonal antisera, however, have some disadvantages. They may lack specificity and vary a great deal, even though the same immunogens are used for their production. In fact, many polyclonal antisera are available and may be used for keratin and other filaments such as actin, vimentin, and myosin and for fibrous proteins such as laminin, fibronectin, collagens, and amyloid.Until recently, there has been only slow progress in the methods for producing specific antibodies. In 1975, Köhler and Milstein described a fundamentally new approach: the production of antibodies by somatic cell hybridization of antibody‐forming cells and continuously replicating cell lines. This technique has allowed preparation of virtually unlimited quantities of antibodies that are chemically, physically, and immunologically completely homogeneous (3–5).MCA have become some of the most widely utilized tools for immunological research. An explosion of MCA makes them available for use in cutaneous immunopathology and the field of application is enlarging very quickly.In this article, some aspects of the production methods of utilization and examples of applications of MCA in cutaneous immunohistochemistry will be discussed.
The Proteoglycans in Hypertrophic Scar
Authors: Honda Tomohito , Matsunaga Etsuji , Katagiri Kazumoto , Shinkai Hiroshi 
Published: April 2015

AbstractSulfated proteoglycans of hypertrophic scar (6 months old after a burn) were extracted with 6 M hot urea, 0.02 M phosphate buffer, pH 7.0, containing protease inhibitors. Two types of proteoglycans, dermatan sulfate proteoglycans and chondroitinsulfate rich proteoglycans, were recovered. The dermatan sulfate proteoglycans, which are capable of binding to concanavalin A, were composed of Mr=53000 core protein and iduronate rich dermatan sulfate. The chondroitin sulfate rich proteoglycans did not bind to concanavalin A but had the same size core proteins as the dermatan sulfate proteoglycans, based on SDS‐polyacrylamide gel electrophoresis. The tryptic peptide mappings showed that the core proteins from dermatan sulfate have the same structural sequences as those from chondroitin sulfate rich proteoglycans.
Distribution Pattern of DNA Polymerases in Epidermis
Authors: Kaneko Noriaki , Tanaka Takaho , Hidaka Toshihiro , Ogura Ryohei 
Published: April 2015

AbstractDNA polymerases are known to play important roles in DNA replication and repair processes. The present study revealed that the DNA polymerase α and β participate in the cell differentiation of normal and n‐hexadecane‐induced hyperplastic epidermis of the guinea pigs. The epidermal cells were separated into three layers; high (HDCL), middle (MDCL), and low (LDCL) density cell layer, respectively, by Percoll gradient centrifugation. In epidermal homogenate, the activity of DNA polymerase β was higher than that of DNA polymerase α. DNA polymerase α activity was higher in the HDCL than in the other layers; however, DNA polymerase β was higher in the LDCL than in the other layers. In hyperplastic epidermis, distribution of DNA polymerase α activity was similar to the normal pattern, but DNA polymerase β was lower in the LDCL than in the other layers. The distribution of DNA polymerase α activity in epidermal nuclei was similar to that of the whole epidermal cell pattern; however, DNA polymerase β activity differed from the enzyme distribution of whole epidermal cell homogenate. Its activity was higher in the HDCL than in the other layers. In hyperplastic epidermis, both nuclear DNA polymerase α and β activities were similar to the distribution patterns of polymerase activities in hyperplastic epidermal cells. From these results, it is concluded that the distribution pattern of DNA polymerases in n‐hexadecane induced‐hyperplastic epidermis is not a simple augmentation of the distribution pattern in normal epidermis.
Antigenic Differences and Further Perspectives on the Differential Diagnosis of Skin Tumors
Authors: Tosca Androniki D. , Lehou Joan , Panagiotopoulos Anthony , Varelzidis Anthony , Stratigos John 
Published: April 2015

AbstractFrozen sections from squamous cell carcinomas (SCCs), basal cell carcinomas (BCCs), precancerous lesions (P.C.) and normal skin adjacent to lesions (N.S.) were studied by indirect immunoperoxidase for the detection of epithelial membrane antigen (EMA) and by PAP (peroxidase‐antiperoxidase) for total keratin (T.K.) and laminin (LM) to determine the significance of the appearance of neoantigens and the behavior of normal epidermal antigens during the process of carcinogenesis of the skin. Significant antigenic differences in all three antigens were revealed between SCCs and BCCs in the epidermis (EP) and tumor islands (T.I.). There were also significant differences in total keratin and laminin between SCC's and N.S. epidermis. SCCs did not show any significant differences in EMA from P.C. lesions, but T.K. was lower in P.C. lesions than in normal skin.
Induction of Chronic Skin Changes in Intraperitoneally Hematoporphyrin‐Injected Mice with Metal Halide Lamp Irradiation
Authors: Honda Tetsuzo , Nonaka Shigeo , Murayama Fumio , Ohgami Taro , Yoshida Hikotaro 
Published: April 2015

AbstractTo induce chronic skin changes simulating human porphyria, mice which had been injected intraperitoneally with hematoporphyrin dihydrochloride were exposed to metal halide lamps. Clinical and histopathological findings in the mice were observed. Clinically, erythema, edema, erosion, necrosis and crust formation were initially seen in all of the mice treated. Hypertrophy of the skin, scarring, and deformities of the ear lobes followed gradually. Histopathological findings in these skin changes were mainly seen in the dermis and included infiltration of lymphocytes, proliferation of mast cells, dilatation and hyperemia of blood vessels in the upper dermis, and swelling and proliferation of connective tissues. PAS positive materials were deposited in the dermo‐epidermal junction and around the small blood vessels in the upper dermis. Using the direct immunofluorescent technique, the deposition of immunoglobulin G (IgG) was confirmed in the same areas, and some deposition of IgM, IgA and C3 was also observed. These findings are similar to those seen in patients with erythropoietic protoporphyria and porphyria cutanea tarda.