bluuebunny/crossref_metadata_2025_split huggingface dataset:
Espacios dinámicos especializados al cultural del folklore ecuatoriano para promover su implementación en el currículum en la U.E María Andrea periodo 2023-2024
Authors: Otacoma Elizondo Nathaly Nicolle , Mora Aristega Angelica , Elizondo León Narcisa Magdalena , Guijarro Intriago Rosa Victoria 
Published: July 2024

El tema Espacios dinámicos especializados al cultural del folklore ecuatoriano para promover su implementación en el currículum en la U.E María Andrea periodo 2023-2024. Este artículo es original y no ha existido previamente un estudio similar dentro de la Facultad. Así, este contribuye a tener un conocimiento teórico - práctico del folklore nacional a través de la danza de proyección folclórica. Para efecto del mismo se han desarrollado varios capítulos encaminados a detallar tanto conceptos teóricos como prácticos, valiéndose de las metodologías apropiadas, planificaciones y técnicas las cuales sugieren los pasos y recursos útiles para su aplicación. Actualmente la Facultad no cuenta con un grupo de danzas folclóricas representativo de la misma, de allí su importancia de crear y conformar este conjunto de danzas. En cuanto al diseño metodológico, presenta un enfoque mixto, debido que al recoger información, se consiguieron datos cualitativos de la población de estudio para transformarlos en datos cuantitativos con porcentajes y frecuencias para tabular, analizar e interpretar, por el nivel de profundidad, responde a un trabajo de investigación exploratorio, descriptivo, de tipo bibliográfico-documental mismos que permitieron conocer la literatura científica de cada variable a través de diversas fuentes bibliográficas para su sustentación teórica y cimentada en una base jurídica.
Utilização dos óculos de realidade virtual durante a sedestação em pacientes com COVID-19 internados em uma Unidade de Terapia Intensiva: Estudo clínico randomizado
Authors: Wassmuth Talita , Lara Eduardo Manoel de , Santos Elaine Becher , Mazzo Débora Melo , Cerutti Diolete Marcante Lati , Pacheco Maurício Zadra , Schleder Juliana Carvalho 
Published: July 2024

Introdução: O internamento prolongado pela COVID-19 gera alterações funcionais, das quais a mobilização de forma precoce com auxílio da Realidade Virtual (RV) recupera e alivia essas condições. Objetivo: Avaliar os efeitos da utilização da RV imersiva sobre o nível de dor, tempo de permanência em sedestação à beira leito e mobilidade de pacientes diagnosticados com COVID-19, internados em Unidades de Terapia Intensiva (UTI). Métodos: Estudo realizado com pacientes diagnosticados com COVID-19 e internados nas UTIs adulto do Hospital Universitário Regional dos Campos Gerais da Universidade Estadual de Ponta Grossa. Os pacientes foram divididos em dois grupos: realidade virtual - sedestados com os óculos de realidade virtual, e o grupo sedestação a beira do leito - sem os óculos. Foram mensurados, dor e mobilidade antes e após a sedestação, e o tempo de permanência nesta posição. Resultados: 40 pacientes foram inclusos, 20 no grupo sedestação a beira leito com média de idade 53,55 anos e 20 no grupo realidade virtual com média de idade de 50,7 anos. Conclusão: O uso da realidade virtual na fisioterapia em pacientes na UTI, em uma única sessão, é segura e proporcionou maior tempo de permanência na posição sedestada, porém não agiu na mobilidade e no controle da dor.
The salivary microbiome as a diagnostic biomarker of periodontitis: a 16S multi-batch study before and after the removal of batch effects
Authors: Regueira-Iglesias Alba , Suárez-Rodríguez Berta , Blanco-Pintos Triana , Relvas Marta , Alonso-Sampedro Manuela , Balsa-Castro Carlos , Tomás Inmaculada 
Published: July 2024

IntroductionMicrobiome-based clinical applications that improve diagnosis related to oral health are of great interest to precision dentistry. Predictive studies on the salivary microbiome are scarce and of low methodological quality (low sample sizes, lack of biological heterogeneity, and absence of a validation process). None of them evaluates the impact of confounding factors as batch effects (BEs). This is the first 16S multi-batch study to analyze the salivary microbiome at the amplicon sequence variant (ASV) level in terms of differential abundance and machine learning models. This is done in periodontally healthy and periodontitis patients before and after removing BEs.MethodsSaliva was collected from 124 patients (50 healthy, 74 periodontitis) in our setting. Sequencing of the V3-V4 16S rRNA gene region was performed in Illumina MiSeq. In parallel, searches were conducted on four databases to identify previous Illumina V3-V4 sequencing studies on the salivary microbiome. Investigations that met predefined criteria were included in the analysis, and the own and external sequences were processed using the same bioinformatics protocol. The statistical analysis was performed in the R-Bioconductor environment.ResultsThe elimination of BEs reduced the number of ASVs with differential abundance between the groups by approximately one-third (Before=265; After=190). Before removing BEs, the model constructed using all study samples (796) comprised 16 ASVs (0.16%) and had an area under the curve (AUC) of 0.944, sensitivity of 90.73%, and specificity of 87.16%. The model built using two-thirds of the specimens (training=531) comprised 35 ASVs (0.36%) and had an AUC of 0.955, sensitivity of 86.54%, and specificity of 90.06% after being validated in the remaining one-third (test=265). After removing BEs, the models required more ASVs (all samples=200–2.03%; training=100–1.01%) to obtain slightly lower AUC (all=0.935; test=0.947), lower sensitivity (all=81.79%; test=78.85%), and similar specificity (all=91.51%; test=90.68%).ConclusionsThe removal of BEs controls false positive ASVs in the differential abundance analysis. However, their elimination implies a significantly larger number of predictor taxa to achieve optimal performance, creating less robust classifiers. As all the provided models can accurately discriminate health from periodontitis, implying good/excellent sensitivities/specificities, the salivary microbiome demonstrates potential clinical applicability as a precision diagnostic tool for periodontitis.
The role of gut microbiota and blood metabolites in postpartum depression: a Mendelian randomization analysis
Authors: Cui Ji , Zhai Qilong , Yang Zhu , Liu Yi 
Published: July 2024

BackgroundPostpartum depression (PPD) is a common complication of pregnancy that imposes a heavy health and economic burden on individuals, families and society. The etiology of PPD is complex and incompletely defined, and recent studies have identified an important role for gut microbiota (GM) and their metabolites in neurological disorders. However, fewer studies on GM and PPD are available and have not yielded uniform results.MethodsInstrumental variables for GM and blood metabolites were obtained from the MiBioGen consortium and metabolomics GWAS server. Single nucleotide polymorphisms (SNPs) associated with PPD phenotypes were obtained from the FinnGen consortium. Inverse variance weighted (IVW), weighted median, weighted mode, and MR-Egger methods were used to assess causal effects. Inverse MR analysis and sensitivity analysis were also utilized to improve the stability of the results.ResultsIn this study, 5 intestinal species and 24 blood metabolites causally associated with PPD were identified using MR analysis. In addition, MR analysis showed that Prevotellaceae and Bifidobacteria may reduce the risk of PPD by elevating Xanthine and 1-arachidonoylglycerophosphoinositol (LysoPI) levels.ConclusionsThis study identified GM and blood metabolites causally associated with PPD. The results of this study may provide a theoretical basis for the discovery of PPD-related biomarkers and the treatment of the disease by regulating the gut microenvironment.
Assessing adherence to treatment guidelines and complications among atrial fibrillation patients in the United Arab Emirates
Authors: Ayash Bayan , Malaeb Diana , Hallit Souheil , Hosseini Hassan 
Published: July 2024

BackgroundAtrial fibrillation (AF), a potential trigger for stroke development, is considered a modifiable condition that can halt complications, decrease mortality, and prevent morbidity. The CHA₂DS₂-VASc and HAS-BLED scores are categorized as risk assessment tools used to estimate the risk of thrombosis development and assess major bleeding among atrial fibrillation patients.ObjectivesOur study aims to assess the adherence to post-discharge treatment recommendations according to CHA₂DS₂-VASc score risk group and evaluate the impact of CHA₂DS₂-VASc score and HAS-BLED score risk categories on death, length of hospital stay, complications, and hospital readmission among United Arab Emirates (UAE) patients.MethodsThis was a multicenter retrospective study conducted from November 2022 to April 2023 in the United Arab Emirates. Medical charts for AF patients were assessed for possible enrolment in the study.ResultsA total number of 400 patients were included with a mean age of 55 (±14.5) years. The majority were females (67.8%), and most had high CHA₂DS₂-VASc and HAS-BLED scores (60% and 57.3%, respectively). Our study showed that adherence to treatment recommendations upon discharge was 71.8%. The bivariate analysis showed that patients with a high CHA₂DS₂-VASc score had a significantly higher risk of death (p-value of 0.001), hospital readmission (p-value of 0.007), and complications (p-value of 0.044) vs. the low and moderate risk group with a p-value of &lt;0.05. Furthermore, our findings showed that the risk of death (0.001), complications (0.057), and mean hospital stay (0.003) were significantly higher in the high HAS-BLED risk score compared to both the low- and moderate-risk categories. Hospital stay was significantly higher in CHA₂DS₂-VASc and HAS-BLED high-risk score categories compared to the low-risk score category with a p-value of &lt;0.001.ConclusionOur study concluded that the adherence to treatment guidelines in atrial fibrillation patients was high and showed that patients received the most effective and patient-centered treatment. In addition, our study concluded that the risk of complications and mortality was higher in high-risk category patients.
Healthcare professionals’ perspectives towards the digitalisation of paediatric growth hormone therapies: expert panels in Italy and Korea
Authors: Rivera Romero Octavio , Chae Hyun Wook , Faienza Maria Felicia , Vergani Edoardo , Cheon Chong Kun , Di Mase Raffaella , Frasca Francesco , Lee Hae Sang , Giavoli Claudia , Kim Jihyun , Klain Antonella , Moon Jung Eun , Iezzi Maria Laura , Yeh James , Aversa Antonio , Rhie Young-Jun , Koledova Ekaterina 
Published: July 2024

IntroductionTo analyse the perspectives of healthcare professionals (HCPs) regarding the acceptance of digital health solutions for growth hormone (GH) deficiency care. This study identified factors impacting HCPs’ intent to use and recommend digital solutions supporting recombinant-human growth hormone (r-hGH) therapy in Italy and Korea with a use case of connected drug delivery system (Aluetta® with Smartdot™) integrated in a platform for GH treatment support (the Growzen™ digital health ecosystem).MethodsParticipatory workshops were conducted in Rome, Italy, and Seoul, Korea, to collect the perspectives of 22 HCPs on various predefined topics. HCPs were divided into two teams, each moderated by a facilitator. The workshops progressed in five phases: introduction of the project and experts, capturing views on the current context of digitalisation, perceived usefulness and ease of use of Aluetta® with Smartdot™, exploration of the perception of health technology evolution, and combined team recommendations. Data shared by HCPs on technology acceptance were independently analysed using thematic analysis, and relevant findings were shared and validated with experts.ResultsHCPs from both Italy and Korea perceived Aluetta® with Smartdot™ and the Growzen™ based digital health ecosystem as user-friendly, intuitive, and easy-to-use solutions. These solutions can result in increased adherence, a cost-effective healthcare system, and medication self-management. Although technology adoption and readiness may vary across countries, it was agreed that using digital solutions tailored to the needs of users may help in data-driven clinical decisions and strengthen HCP–patient relationships.ConclusionHCPs’ perspectives on the digitalisation in paediatric GH therapies suggested that digital solutions enable automatic, real-time injection data transmission to support adherence monitoring and evidence-based therapy, strengthen HCP–patient relationships, and empower patients throughout the GH treatment process.
Novel genotypes and phenotypes in Snijders Blok-Campeau syndrome caused by CHD3 mutations
Authors: Gao Yuanyuan , Wang Pei , Chen Mengying , Pang Kexin , Sun Yifan , Zheng Bixia , Li Taisong , Zhang Hongmei , Zhu Min 
Published: July 2024

BackgroundSnijders Blok-Campeau syndrome (SNIBCPS) is a rare genetic disorder characterized by facial abnormalities, hypotonia, macrocephaly, and global developmental delay (GDD) caused by mutations in CHD3 gene. There is limited information on SNIBCPS and few studies on its pathogenic gene CHD3.MethodsWe utilized whole-exome sequencing, in vitro minigene splicing assay analysis, and construction of protein models to validate the suspected pathogenic mutation. In addition, the PubMed database was searched using the keywords “Snijders Blok-Campeau syndrome,” “CHD3,” or “SNIBCPS” to summarize the gene mutations and clinical phenotypic characteristics of children with SNIBCPS.ResultsWe identified a non-frameshift variant c.3592_c.3606delGCCAAGAGAAAGATG, a splice site variant c.1708-1G&gt;T, and two missense variants, c. 2954G&gt;C (p.Arg985Pro) and c.3371C&gt;T (p.A1124V), in CHD3 variants with SNIBCPS. Importantly, the c.3592_c.3606delGCCAAGAGAAAGATG, c.1708-1G&gt;T, and c.3371C &gt; T (p.A1124V) loci were not reported, and the children in this study also had phenotypic features of unibrow, transverse palmar creases, tracheal bronchus, and hypomelanosis of Ito (HI). The c.1708-1G&gt;T classical splicing mutation leads to abnormal shearing of mRNA, forming a truncated protein that ultimately affects gene function.ConclusionOur findings have expanded the spectrum of genetic variants and clinical features in children with SNIBCPS. Splicing analysis of CHD3 is an important method to understand the pathogenesis of spliced cells.
Identification of core genes in intervertebral disc degeneration using bioinformatics and machine learning algorithms
Authors: Zhang Hao , Shi Shengbo , Huang Xingxing , Gong Changsheng , Zhang Zijing , Zhao Zetian , Gao Junxiao , Zhang Meng , Yu Xiaobing 
Published: July 2024

BackgroundIntervertebral Disc Degeneration (IDD) is a major cause of lower back pain and a significant global health issue. However, the specific mechanisms of IDD remain unclear. This study aims to identify key genes and pathways associated with IDD using bioinformatics and machine learning algorithms.MethodsGene expression profiles, including those from 35 LDH patients and 43 healthy volunteers, were downloaded from the GEO database (GSE124272, GSE150408, GSE23130, GSE153761). After merging four microarray datasets, differentially expressed genes (DEGs) were selected for GO and KEGG pathway enrichment analysis. Weighted Gene Co-expression Network Analysis (WGCNA) was then applied to the merged dataset to identify relevant modules and intersect with DEGs to discover candidate genes with diagnostic value. A LASSO model was established to select appropriate genes, and ROC curves were drawn to elucidate the diagnostic value of genetic markers. A Protein-Protein Interaction (PPI) network was constructed and visualized to determine central genes, followed by external validation using qRT-PCR.ResultsDifferential analysis of the preprocessed dataset identified 244 genes, including 183 upregulated and 61 downregulated genes. WGCNA analysis revealed the most relevant module intersecting with DEGs, yielding 9 candidate genes. The lasso-cox method was used for regression analysis, ultimately identifying 6 genes: ASPH, CDC42EP3, FOSL2, IL1R1, NFKBIZ, TCF7L2. A Protein-Protein Interaction (PPI) network created with GENEMANIA identified IL1R1 and TCF7L2 as central genes.ConclusionOur study shows that IL1R1 and TCF7L2 are the core genes of IDD, offering new insights into the pathogenesis and therapeutic development of IDD.
Overlap of membranous nephropathy and IgA nephropathy in a patient with Kimura’s disease: a case report and literature review
Authors: Barbosa Géssica Sabrine Braga , Neves Precil Diego Miranda de Menezes , Mohrbacher Sara , Abdo André Néder Ramires , Cavalcante Lívia Barreira , Menezes Yara de , Sato Victor Augusto Hamamoto , Oliveira Érico de Souza , Pereira Leonardo Victor Barbosa , Bales Alessandra Martins , Frediani Marcella Martins , Chocair Pedro Renato , Cuvello-Neto Américo Lourenço 
Published: July 2024

IntroductionKimura’s disease (KD) is a rare chronic inflammatory disorder characterized by subcutaneous lymphoid hyperplasia with peripheral eosinophilia. Kidney involvement is reported in 15%–18% of adult patients with KD, in many cases as nephrotic syndrome. We present a case of overlapping membranous nephropathy and IgA nephropathy associated with KD.Case reportA 27-year-old man was admitted with a history of bilateral leg edema for the last 2 months and concomitant progressive increase of cervical mass and fever. Laboratory findings were as follows: peripheral leukocyte count, 10,080/mm³; eosinophils, 3,200/mm³ (31.7%); serum creatinine, 0.83 mg/dL; and eGFR: 140 mL/min per 1.73 m2. Urinalysis revealed the presence of hematuria and proteinuria and the following results: 24-h proteinuria, 12.9 g; serum albumin, 1.3 g/dL; and elevated IgE level, 750 kU/L. Serologies for hepatitis B, hepatitis C, HIV, and VDRL were all negative. Complement C3 and C4 levels were normal. No monoclonal protein was detected in blood and urine. Parasite infestation was discarded. A biopsy of the cervical lymph node revealed eosinophilic lymphoid hyperplasia, suggesting KD. A kidney biopsy revealed findings consistent with the overlapping of membranous nephropathy with IgA nephropathy. The patient was treated for KD with prednisone 1 mg/kg/d with progressive dose tapering and posterior association of methotrexate 15 mg/week. A renin–angiotensin system inhibitor was prescribed for nephrotic syndrome. The cervical mass regressed, and proteinuria achieved partial remission, with an increase in serum albumin level and normalization of eosinophils and IgE levels.ConclusionAlthough uncommon, kidney involvement must be considered in patients with KD. Glomerular diseases are the most frequent form of kidney injury.
Patient-reported assessment of compassion in Spanish: a systematic review
Authors: Soto-Rubio Ana , Picazo Carmen , Gil-Juliá Beatriz , Andreu-Vaillo Yolanda , Pérez-Marín Marian , Sinclair Shane 
Published: July 2024

Aims and objectivesThis systematic review aims to: (1) explore which tools have been used in Spanish to measure compassion; (2) know which of these tools could be used to assess compassion in healthcare settings from the perspective of patients; (3) evaluate the quality of these patient-reported measures in Spanish contexts; and (4) determine which of these instruments would be best suited to be used in healthcare settings.BackgroundCompassion has been recognized as a fundamental dimension of quality healthcare.MethodsSeveral scientific databases were consulted for relevant records published up to December 16th, 2021. In accordance with PRISMA guidelines, 64 studies were included.Results and conclusionswhile existing instruments, validated in Spanish, allow for the measurement of self-compassion or compassion to others, there are no valid and reliable measures currently available in Spanish to measure patient-reported compassion.Relevance to clinical practiceIn order to ensure and promote compassion in the health care context, it is essential to have a valid and reliable tool to measure this construct in a patient-informed way, and this is currently not possible in the Spanish-speaking context because of the lack of such an instrument in Spanish.
Exploring prebiotic properties and its probiotic potential of new formulations of soy milk-derived beverages
Authors: Rana Ananya , Taneja Neetu Kumra , Raposo António , Alarifi Sehad N. , Teixeira-Lemos Edite , Lima Maria João , Gonçalves João Carlos , Dhewa Tejpal 
Published: July 2024

IntroductionThe food and beverage industry has shown a growing interest in plant-based beverages as alternatives to traditional milk consumption. Soy milk is derived from soy beans and contains proteins, isoflavones, soy bean oligosaccharides, and saponins, among other ingredients. Because of its high nutritive value and versatility, soy milk has gained a lot of attention as a functional food.MethodsThe present work aims to explore the prebiotic properties and gastrointestinal tolerance potential of new formulations of soy milk-derived drinks to be fermented with riboflavin-producing probiotic Lactiplantibacillus plantarum MTCC (Microbial Type Culture Collection and Gene Bank) 25432, Lactiplantibacillus plantarum MTCC 25433, and Lactobacillus acidophilus NCIM (National Collection of Industrial Microorganisms) 2902 strains.Results and discussionThe soy milk co-fermented beverage showed highest PAS (1.24 ± 0.02) followed by soy milk beverages fermented with L. plantarum MTCC 25433 (0.753 ± 0.0) when compared to the commercial prebiotic raffinose (1.29 ± 0.01). The findings of this study suggested that the soy milk beverages exhibited potent prebiotic activity, having the ability to support the growth of probiotics, and the potential to raise the content of several bioactive substances. The higher prebiotics activity score showed that the higher the growth rate of probiotics microorganism, the lower the growth of pathogen. For acidic tolerance, all fermented soy milk managed to meet the minimal requirement of 106 viable probiotic cells per milliliter at pH 2 (8.13, 8.26, 8.30, and 8.45 logs CFU/mL, respectively) and pH 3.5 (8.11, 8.07, 8.39, and 9.01 log CFU/mL, respectively). The survival rate of soy milk LAB isolates on bile for 3 h ranged from 84.64 to 89.60%. The study concluded that lactobacilli could thrive in gastrointestinal tract. The sensory evaluation scores for body and texture, color, flavor, and overall acceptability showed a significant difference (p &lt; 0.05) between the fermented probiotic soy milk and control samples. Soy milk fermented with a combination of L. plantarum MTCC 25432 &amp; MTCC 25433 demonstrated the highest acceptability with the least amount of beany flavor. The findings of the study suggest soy milk’s potential in plant-based beverage market.