bluuebunny/crossref_metadata_2025_split huggingface dataset:
Movements of female exurban white‐tailed deer in response to controlled hunts
Authors: Rhoads Craig L. , Bowman Jacob L. , Eyler Brian 
Published: June 2013

ABSTRACTWhite‐tailed deer (Odocoileus virginianus) thrive in exurban habitats, which results in increased occurrences of deer–human conflicts. Controlled hunts are one method currently used by managers to deal with increasing exurban deer populations. To develop successful controlled hunts, managers must understand how deer respond to hunting pressure during controlled hunts. Previous research has documented a varied response of deer to harvest pressure and this response could affect the efficacy of a controlled hunt. We investigated deer responses to controlled hunts by examining an exurban deer population residing on Fair Hill Natural Resource Management Area in Cecil County, Maryland, USA. During controlled hunts in 2005 and 2006, we collected locations on 74 collared adult female deer. The percentage of deer available for harvest decreased 20–25% from before to after the hunt, because deer moved to refuges. Deer in refuges had similar movement rates before, during, and after the hunt, and these movement rates were similar to deer in non‐refuge areas before the hunt. Deer in non‐refuge areas increased their movement rates 12.6–15.6 m/hour during the hunt and returned to before‐hunt levels after the hunt. Our results suggest that refuges may provide sanctuary to a substantial portion of exurban deer during controlled hunts. To maximize deer harvests during controlled hunts, managers should identify and attempt to eliminate as many refuge areas as possible and plan multiple short‐duration hunts to reduce the impact of deer moving into refuge areas. © 2013 The Wildlife Society.
Evaluation of a Heredofamilial Cancer Unit in Increasing Family History Collection and Genetic Counseling Referrals Among Spanish Oncologists at a University Hospital
Authors: Márquez‐Rodas Iván , López‐Tarruella Sara , Jerez Yolanda , Cavanagh Mercedes , Custodio Sara , López‐Trabada Daniel , Moya Beatriz , Pérez Sara , Rupérez Ana B. , Martín Miguel 
Published: June 2013

AbstractA comprehensive family history is essential to identify patients at risk for hereditary cancer who could benefit from genetic counseling (GC). In a previous study, we observed a low occurrence of family history record (FHR) collection rate and GC referral among oncologists at our institution. The present work analyzes whether the implementation of a heredofamilial cancer unit (HFCU) would improve these parameters. We retrospectively compared the FHR rate in clinical records, National Cancer Institute (NCI) general criteria for hereditary cancer suspicion, GC referrals and FHR quality in two cohorts: cohort 1 (patients diagnosed before HFCU creation) and cohort 2 (after HFCU creation). Of 1,175 patients (590 cohort 1 and 585 cohort 2), FHRs were consigned in 27.3 % and 52.5 % of patients, respectively (p < 0.001). The GC referral of patients with any NCI criterion was 13.6 % xin cohort 1 vs. 40.5 % in cohort 2 (p < 0.001). FHR quality improved in terms of the total number of relatives (164 vs. 314, p = 0.1, N.S.) and number of healthy relatives consigned (80 vs. 191, p < 0.01). Nine mutations (6 BRCA, 1 MEN1, 2 Lynch), 4 unknown significance variants (all in BRCA) and 2 with no mutation were identified among patients referred from cohort 2. We conclude that the creation of a heredofamilial cancer unit has changed both FHR and GC referrals among oncologists at our institution, although continuous educational efforts are required.
Typhoon-dominated influence on wood debris distribution and transportation in a high gradient headwater catchment
Authors: Chen Su-Chin , Chao Yi-Chiung , Chan Hsun-Chuan 
Published: June 2013

Abstract
Wood debris is an important component of mountain streams. It causes serious damage and renders difficulty of water resource management in Taiwan. In this study, the quantity of wood debris and variation of migratory wood debris during flood events were examined. The downstream of Gaoshan Creek and Qijiawan Creek, located at Central Taiwan, was selected as the study area. The distribution and dynamic of wood debris in a high gradient headwater catchment were quantified using field surveys. A formula of critical depth for wood debris entrainment was used to evaluate the wood debris migration during three flooding events. In the study area, wood abundance and unit volume increased downstream, and wood density decreased downstream within a channel network. The channel morphology, riparian vegetation, and wood debris characteristics were found to influence the wood storage. As a result, the wood debris has an irregular accumulative distribution in the steep stream, and it migrates easily in the stream because of a high flow discharge. Strong relationships between the channel width and wood debris variables are discovered. Moreover, wood debris has a tendency to accumulate at sites with low stream power and wood debris dams, topographical notches, and unique geological structures. Our findings assist in the understanding of the effects of channel characteristics on distributions of wood debris in steep stream systems.
Physicochemical Properties, Volatile Compounds and Phospholipid Classes of Silver Carp Brain Lipids
Authors: Wang Caixia , Xia Wenshui , Xu Yanshun , Jang Qixing , Yu Peipie 
Published: June 2013

AbstractLarge amounts of utilizable by‐products are produced concomitant with silver carp processing. The lipids from fish brain contain considerable essential fatty acids which however have seldom been studied. In this study, the lipids extracted from the brain of silver carp exhibited good physicochemical properties. Forty volatile compounds were identified, of which hydrocarbons and aromatics predominated. The ratios of polar lipids and phospholipids to total lipids were 22.73 and 10.95 % respectively. Phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) accounted for 83.9 % of the phospholipids. Major fatty acids of neutral lipids, polar lipids, PC, PE and PS differed significantly. PE had the most polyunsaturated fatty acids (PUFA), while PC, PE and PS were rich in docosahexaenoic and eicosapentaenoic acids. Moreover, the ratios of fatty acids were considered to be reasonable due to high levels of n3 PUFA. The lipids from silver carp brain have been demonstrated to be healthy and nutritious. The results herein confirm that silver carp brain lipids are a commercially and industrially feasible prospect.
Riluzole increases glutamate uptake by cultured C6 astroglial cells
Authors: Dall'Igna Oscar P. , Bobermin Larissa D. , Souza Diogo O. , Quincozes‐Santos André 
Published: June 2013

AbstractRiluzole is a drug approved for the treatment of amyotrophic lateral sclerosis (ALS) and may be effective for the treatment of other neurodegenerative and neuropsychiatric disorders. Riluzole exerts diverse actions on the central nervous system, including altering glutamate release and uptake, and therefore act diminishing glutamate extracellular levels, but the underlying mechanism of these actions is still unknown. Here, we demonstrate that riluzole stimulated glutamate uptake and augmented the expression of the glutamate EAAC1 transporter in C6 astroglial cell cultures. The effect of riluzole on glutamate uptake was reduced to below controls when it was co‐administered with inhibitors of protein kinase C (PKC; bisindolylmaleimide II), phosphatidylinositol 3‐kinase (PI3K; wortmannin) and fibroblast growth factor receptor 1 (FGFR1; PD173074). Riluzole also decreased reactive oxygen species load with no effect on glutathione levels. This study investigates three independent intracellular pathways and the mechanism of action of riluzole on glutamate metabolism.
Through the open door: Preferential binding of dasatinib to the active form of BCR‐ABL unveiled by in silico experiments
Authors: Laurini Erik , Posocco Paola , Fermeglia Maurizio , Gibbons Don L. , Quintás-Cardama Alfonso , Pricl Sabrina 
Published: June 2013

Dasatinib is a second‐generation BCR‐ABL inhibitor approved for the treatment of patients with chronic myeloid leukemia, both in the frontline and in the imatinib‐resistant/intolerant settings. The high affinity of dasatinib for the protein is currently assumed to result from its ability to bind both the active and inactive conformations of the BCR‐ABL kinase. In the present work, using state of the art molecular simulation techniques we prove that dasatinib exhibits a highly selective preference for the active (open) BCR‐ABL conformation. By using three different BCR‐ABL conformations (active, inactive, and intermediate inactive) we show that, from a thermodynamic standpoint, the affinity of dasatinib for BCR‐ABL drastically decreases in the order: active > alternative inactive > inactive, as a result of differential contributions from the single residues lining the kinase binding pocket and the concomitant stabilization/destabilization of the kinase hydrophobic spine. Molecule‐pulling experiments also corroborate this trend as significantly lower forces and smaller times are required to extract dasatinib from its inactive BCR‐ABL complexes with respect to the active complex counterparts. Importantly, our results support recent NMR solution results demonstrating no evidence of dasatinib bound to the inactive form of BCR‐ABL.
Consumer Response to “Contains” and “Free of” Labeling: Evidence from Lab Experiments
Authors: Liaukonyte Jura , Streletskaya Nadia A. , Kaiser Harry M. , Rickard Bradley J. 
Published: June 2013

AbstractUsing a lab experiment with 351 adult non‐student subjects, we investigate the impact of labels and secondary information on willingness to pay (WTP) for foods that use various ingredients and processes that have been the subject of food policy discussions. We find a distinct asymmetry of WTP sensitivity between “Contains X” and “Free of X” labels with negatively‐framed secondary information. The “Free of X” label has an impact only when secondary information is provided, and the negative impact of “Contains X” is mitigated by secondary information. We also consider how the results of our study can inform the ongoing debate about mandatory food labeling regulations in the United States: if mandatory labeling is adopted, providing additional information about what the product contains would significantly lessen the negative impact on demand.
AdvISER-PYRO: Amplicon Identification using SparsE Representation of PYROsequencing signal
Authors: Ambroise Jérôme , Piette Anne-Sophie , Delcorps Cathy , Rigouts Leen , de Jong Bouke C. , Irenge Leonid , Robert Annie , Gala Jean-Luc 
Published: June 2013

AbstractMotivation: Converting a pyrosequencing signal into a nucleotide sequence appears highly challenging when signal intensities are low (unitary peak heights ) or when complex signals are produced by several target amplicons. In these cases, the pyrosequencing software fails to provide correct nucleotide sequences. Accordingly, the objective was to develop the AdvISER-PYRO algorithm, performing an automated, fast and reliable analysis of pyrosequencing signals that circumvents those limitations.Results: In the current mycobacterial amplicon genotyping application, AdvISER-PYRO performed much better than the pyrosequencing software in the following two situations: when converting Single Amplicon Sample (SAS) signals into a correct single sequence (97.2% versus 56.5%), and when translating Multiple Amplicon Sample (MAS) signals into the correct sequence pair (74.5%).Availability: AdvISER-PYRO is implemented in an R package (http://sites.uclouvain.be/md-ctma/index.php/softwares) and can be used in broad range of clinical applications including multiplex pyrosequencing and oncogene re-sequencing in heterogeneous tumor cell samples.Contact: jerome.ambroise@uclouvain.be or jean-luc.gala@uclouvain.be
Porter, PaleAle 4.0: high-accuracy prediction of protein secondary structure and relative solvent accessibility
Authors: Mirabello Claudio , Pollastri Gianluca 
Published: June 2013

Abstract
Summary: Protein secondary structure and solvent accessibility predictions are a fundamental intermediate step towards protein structure and function prediction. We present new systems for the ab initio prediction of protein secondary structure and solvent accessibility, Porter 4.0 and PaleAle 4.0. Porter 4.0 predicts secondary structure correctly for 82.2% of residues. PaleAle 4.0’s accuracy is 80.0% for prediction in two classes with a 25% accessibility threshold. We show that the increasing training set sizes that come with the continuing growth of the Protein Data Bank keep yielding prediction quality improvements and examine the impact of protein resolution on prediction performances.
Availability: Porter 4.0 and PaleAle 4.0 are freely available for academic users at http://distillf.ucd.ie/porterpaleale/. Up to 64 kb of input in FASTA format can be processed in a single submission, with predictions now being returned to the user within a single web page and, optionally, a single email.
Contact: gianluca.pollastri@ucd.ie
Feeding herring schools do not react to seismic air gun surveys
Authors: Peña Héctor , Handegard Nils Olav , Ona Egil 
Published: June 2013

Abstract
Peña, H., Handegard, N. O. and Ona, E. 2013. Feeding herring schools do not react to seismic air gun surveys. – ICES Journal of Marine Science, 70: 1174–1180. The real-time behaviour of herring schools exposed to a full-scale 3D seismic survey off Vesterålen, northern Norway, was observed using an omnidirectional fisheries sonar. Throughout the study period, the herring swam slowly against the predominant northeast current, with a net displacement along with the current. The mean swimming speed after subtracting the drift velocities was 0.35 m s−1, and the mean response speed in the direction away from the air gun array was 0.22 m s−1. No changes were observed in swimming speed, swimming direction, or school size that could be attributed to the transmitting seismic vessel as it approached from a distance of 27 to 2 km, over a 6 h period. The unexpected lack of a response to the seismic survey was interpreted as a combination of a strong motivation for feeding, a lack of suddenness of the air gun stimulus, and an increased level of tolerance to the seismic shooting.
Isoflurane, a Commonly Used Volatile Anesthetic, Enhances Renal Cancer Growth and Malignant Potential via the Hypoxia-inducible Factor Cellular Signaling Pathway In Vitro
Authors: Benzonana Laura L. , Perry Nicholas J. S. , Watts Helena R. , Yang Bob , Perry Iain A. , Coombes Charles , Takata Masao , Ma Daqing 
Published: June 2013

Abstract

Background:
Growing evidence indicates that perioperative factors, including choice of anesthetic, affect cancer recurrence after surgery although little is known about the effect of anesthetics on cancer cells themselves. Certain anesthetics are known to affect hypoxia cell signaling mechanisms in healthy cells by up-regulating hypoxia-inducible factors (HIFs). HIFs are also heavily implicated in tumorigenesis and high levels correlate with poor prognosis.

Methods:
Renal cell carcinoma (RCC4) cells were exposed to isoflurane for 2 h at various concentrations (0.5–2%). HIF-1α, HIF-2α, phospho-Akt, and vascular endothelial growth factor A levels were measured by immunoblotting at various time points (0–24 h). Cell migration was measured across various components of extracellular matrix, and immunocytochemistry was used to analyze proliferation rate and cytoskeletal changes.

Results:
Isoflurane up-regulated levels of HIF-1α and HIF-2α and intensified expression of vascular endothelial growth factor A. Exposed cultures contained significantly more cells (1.81 ± 0.25 vs. 1.00 of control; P = 0.03) and actively proliferating cells (89.4 ± 2.80 vs. 64.74 ± 7.09% of control; P = 0.016) than controls. These effects were abrogated when cells were pretreated with the Akt inhibitor, LY294002. Exposed cells also exhibited greater migration on tissue culture–coated (F = 16.89; P = 0.0008), collagen-coated (F = 20.99; P = 0.0003), and fibronectin-coated wells (F = 8.21; P = 0.011) as along with dramatic cytoskeletal rearrangement, with changes to both filamentous actin and α-tubulin.

Conclusions:
These results provide evidence that a frequently used anesthetic can exert a protumorigenic effect on a human cancer cell line. This may represent an important contributory factor to high recurrence rates observed after surgery.
A Role for APETALA1/FRUITFULL Transcription Factors in Tomato Leaf Development
Authors: Burko Yogev , Shleizer-Burko Sharona , Yanai Osnat , Shwartz Ido , Zelnik Iris Daphne , Jacob-Hirsch Jasmine , Kela Itai , Eshed-Williams Leor , Ori Naomi 
Published: June 2013

AbstractFlexible maturation rates underlie part of the diversity of leaf shape, and tomato (Solanum lycopersicum) leaves are compound due to prolonged organogenic activity of the leaf margin. The CINCINNATA -TEOSINTE BRANCHED1, CYCLOIDEA, PCF (CIN-TCP) transcription factor LANCEOLATE (LA) restricts this organogenic activity and promotes maturation. Here, we show that tomato APETALA1/FRUITFULL (AP1/FUL) MADS box genes are involved in tomato leaf development and are repressed by LA. AP1/FUL expression is correlated negatively with LA activity and positively with the organogenic activity of the leaf margin. LA binds to the promoters of the AP1/FUL genes MBP20 and TM4. Overexpression of MBP20 suppressed the simple-leaf phenotype resulting from upregulation of LA activity or from downregulation of class I knotted like homeobox (KNOXI) activity. Overexpression of a dominant-negative form of MBP20 led to leaf simplification and partly suppressed the increased leaf complexity of plants with reduced LA activity or increased KNOXI activity. Tomato plants overexpressing miR319, a negative regulator of several CIN-TCP genes including LA, flower with fewer leaves via an SFT-dependent pathway, suggesting that miR319-sensitive CIN-TCPs delay flowering in tomato. These results identify a role for AP1/FUL genes in vegetative development and show that leaf and plant maturation are regulated via partially independent mechanisms.
Clinical Application of Adult Stem Cells for Therapy for Cardiac Disease
Authors: Ghodsizad Ali , Ruhparwar Arjang , Bordel Viktor , Mirsaidighazi Ebrahim , Klein Hans Michael , Koerner Michael M. , Karck Matthias , El‐Banayosy Aly 
Published: June 2013

SummaryIntroductionCardiovascular disease is a major cause of death worldwide. Different medical and surgical therapeutic options are well established, but a significant number of patients are not amenable to standard therapeutic options. Cell‐based therapies after clinical application have shown different results in recent years. Here, we are giving a comprehensive overview on major available clinical data regarding cell therapy.BackgroundCell‐based therapies and tissue engineering provide new promising platforms to develop upcoming therapeutic options. Initial clinical trials were able to generate promising results. A variety of different stem cell types have been used for the clinical application. Different adult cardiac stem cells and progenitor cells, including mesenchymal, CD34+ and CD133+ autologous human bone marrow–derived stem cells (BMCs), human myoblasts, and peripheral blood–derived stem and progenitor cells (PBSCs) have been used for the therapy for end‐stage heart failure. Future experiments will show the importance of novel cell populations and clarify the mechanism causing cell therapy–mediated observed effects.ConclusionSeveral clinical trials have reported on sole therapy, as well as combined application of autologous adult stem cells with conventional revascularization. The reported promising findings encourage further research in the field of the translational research.
Could donor multipotent mesenchymal stromal cells prevent or delay the onset of diabetic retinopathy?
Authors: Ezquer Fernando , Ezquer Marcelo , Arango‐Rodriguez Martha , Conget Paulette 
Published: June 2013

Abstract.Diabetes mellitus is a complex metabolic disease that has become a global epidemic with more than 285 million cases worldwide. Major medical advances over the past decades have substantially improved its management, extending patients’ survival. The latter is accompanied by an increased risk of developing chronic macro‐ and microvascular complications. Amongst them, diabetic retinopathy (DR) is the most common and frightening. Furthermore, during the past two decades, it has become the leading cause of visual loss. Irrespective of the type of diabetes, DR follows a well‐known clinical and temporal course characterized by pericytes and neuronal cell loss, formation of acellular‐occluded capillaries, occasional microaneurysms, increased leucostasis and thickening of the vascular basement membrane. These alterations progressively affect the integrity of retinal microvessels, leading to the breakdown of the blood–retinal barrier, widespread haemorrhage and neovascularization. Finally, tractional retinal detachment occurs leading to blindness. Nowadays, there is growing evidence that local inflammation and oxidative stress play pivotal roles in the pathogenesis of DR. Both processes have been associated with pericytes and neuronal degeneration observed early during DR progression. They may also be linked to sustained retinal vasculature damage that results in abnormal neovascularization. Currently, DR therapeutic options depend on highly invasive surgical procedures performed only at advanced stages of the disease, and which have proved to be ineffective to restore visual acuity. Therefore, the availability of less invasive and more effective strategies aimed to prevent or delay the onset of DR is highly desirable. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), are promising healing agents as they contribute to tissue regeneration by pleiotropic mechanisms, with no evidence of significant adverse events. Here, we revise the pathophysiology of DR to identify therapeutic targets for donor MSCs. Also, we discuss whether an MSC‐based therapy could prevent or delay the onset of DR.
Senile retinoschisis versus retinal detachment, the additional value of peripheral retinal OCT scans (SL SCAN‐1, Topcon)
Authors: Stehouwer Marilette , Tan Stevie H. , van Leeuwen Ton G. , Verbraak Frank D. 
Published: June 2013

Abstract.Purpose:  A peripheral senile retinoschisis is sometimes difficult to distinguish from a retinal detachment by biomicroscopy alone. This study evaluated spectral domain OCT (SD‐OCT) scans of the far peripheral retina to improve the differentiation between these diseases.Methods:  Patients were included in whom the distinction between retinal detachment and senile retinoschisis was not clear based on biomicroscopy alone, or who had a presumed clinical diagnosis of senile retinoschisis. OCT scans were made with the SLSCAN‐1, through a hand‐held lens and a 3‐mirror contact lens, while the area of interest was simultaneously observed with the slit lamp. The SLSCAN‐1 is a SD‐OCT integrated into a slit lamp, superluminescent diode light source with a central wavelength of 830 nm, bandwidth 30 nm, scan resolution 8–9 μm in tissue, scan depth 2 mm, scan speed 5000 A‐scans per second.Results:  In the 11 patients with an uncertain diagnosis, OCT scans showed a senile retinoschisis in four and a retinal detachment in seven patients. Fifteen of the 18 patients with a presumed diagnosis of retinoschisis showed a retinoschisis on OCT. In five of them, the OCT scans revealed a schisis detachment. Interestingly, three of the 18 patients had a retinal detachment.Conclusion:  Senile retinoschisis may mimic a peripheral retinal detachment and vice versa. Differentiation is important with respect to therapeutic decisions. OCT scans of the far peripheral retina, which can be made with the SLSCAN‐1 through a 3‐mirror contact lens, can contribute significantly to this differentiation. The obtained scans of the far peripheral retina clearly visualize the anatomic differences between both diseases.