bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment of recurrent intracranial aneurysms with the Pipeline Embolization Device
Authors: Chalouhi Nohra , Chitale Rohan , Starke Robert M , Jabbour Pascal , Tjoumakaris Stavropoula , Dumont Aaron S , Rosenwasser Robert H , Gonzalez L Fernando 
Published: January 2013

BackgroundThe treatment of recurrent aneurysms after previous surgery or embolization is challenging. Little is known regarding the use of the Pipeline Embolization Device (PED) for recurrent aneurysms.ObjectiveTo analyze the safety and results of PED therapy for recurrent aneurysms.MethodsFifteen patients with recurrent intracranial aneurysms after previous embolization or surgical clipping were treated with the PED at our institution between 2011 and 2012. Procedural complications and clinical and angiographic outcomes were analyzed.ResultsMedian aneurysm size was 12 mm. Previous aneurysm treatment consisted of coiling in eight patients, stent coiling in four, a telescoping stent technique in two and surgical clipping in one. Major procedural complications (leaving significant morbidity) occurred in one patient (6.7%) and minor procedural or technical complications (no or minor morbidity) occurred in four patients (26.7%). Fourteen of the 15 patients (93.3%) had a favorable outcome (modified Rankin Scale score 0–2). Of 14 patients with angiographic follow-up, nine (64.3%) had complete aneurysm occlusion (100%), four (28.6%) had near-complete occlusion (≥90%) and only one (7.1%) had incomplete occlusion (<90%). Four of the five patients with less than 100% occlusion at follow-up had a previous stent in situ.ConclusionsTreatment of recurrent aneurysms with the PED appears to be effective, but patients with a previous stent in situ may achieve lower obliteration rates. The morbidity rate associated with PED therapy may be higher than with more standard endovascular techniques using historical data. Larger studies are needed to assess this question better.
Multimodal Elucidation of Choline Metabolism in a Murine Glioma Model Using Magnetic Resonance Spectroscopy and 11C-Choline Positron Emission Tomography
Authors: Wehrl Hans F. , Schwab Julian , Hasenbach Kathy , Reischl Gerald , Tabatabai Ghazaleh , Quintanilla-Martinez Leticia , Jiru Filip , Chughtai Kamila , Kiss Andras , Cay Funda , Bukala Daniel , Heeren Ron M.A. , Pichler Bernd J. , Sauter Alexander W. 
Published: January 2013

Abstract
The metabolites, transporters, and enzymes involved in choline metabolism are regarded as biomarkers for disease progression in a variety of cancers, but their in vivo detection is not ideal. Both magnetic resonance spectroscopy [MRS using chemical shift imaging (CSI) total choline (tCho)] and 11C-choline positron emission tomography (PET) can probe this pathway, but they have not been compared side by side. In this study, we used the spontaneous murine astrocytoma model SMA560 injected intracranially into syngeneic VM/Dk mice, analyzing animals at various postimplantation time points using dynamic microPET imaging and CSI MRS. We observed an increase in tumor volume and 11C-choline uptake between days 5 and 18. Similarly, tCho levels decreased at days 5 to 18. We found a negative correlation between the tCho and PET results in the tumor and a positive correlation between the tCho tumor-to-brain ratio and choline uptake in the tumor. PCR results confirmed expected increases in expression levels for most of the transporters and enzymes. Using MRS quantification, a good agreement was found between CSI and 11C-choline PET data, whereas a negative correlation occurred when CSI was not referenced. Thus, 11C-choline PET and MRS methods seemed to be complementary in strengths. While advancing tumor proliferation caused an increasing 11C-choline uptake, gliosis and inflammation potentially accounted for a high peritumoral tCho signal in CSI, as supported by histology and secondary ion mass spectrometry imaging. Our findings provide definitive evidence of the use of MRS, CSI, and PET for imaging tumors in vivo. Cancer Res; 73(5); 1470–80. ©2012 AACR.
Heterogeneity of Neoplastic Stem Cells: Theoretical, Functional, and Clinical Implications
Authors: Valent Peter , Bonnet Dominique , Wöhrer Stefan , Andreeff Michael , Copland Mhairi , Chomienne Christine , Eaves Connie 
Published: January 2013

Abstract
Accumulating evidence suggests that human cancers develop through a step-wise, but nonlinear process of cellular diversification and evolution. Recent mutational analyses indicate that this process is more complex and diverse than anticipated before whole-genome sequencing methods were readily available. Examples are also emerging now of genetically abnormal clones of cells that have acquired mutations with known oncogenic potential but, nevertheless, may show no manifestations of malignant change for many years. To accommodate these diverse realities, we suggest the term neoplastic refer to clones of cells that have any type of somatic aberrancy associated with an increased propensity to become malignant, and the derivative term neoplastic stem cell be adopted to identify the cells responsible for the long-term maintenance of such clones. Neoplastic clones would thus include those that never evolve further, as well as those that eventually give rise to fully malignant populations, and all stages in between. The term cancer stem cells would then be more appropriately restricted to cells generating subclones that have established malignant properties. More precise molecular understanding of the different stem cell states thus distinguished should contribute to the development of more effective prognostic and therapeutic tools for cancer diagnosis and treatment. Cancer Res; 73(3); 1037–45. ©2012 AACR.
Neurotransmitter Substance P Mediates Pancreatic Cancer Perineural Invasion via NK-1R in Cancer Cells
Authors: Li Xuqi , Ma Guodong , Ma Qingyong , Li Wei , Liu Jiangbo , Han Liang , Duan Wanxing , Xu Qinhong , Liu Han , Wang Zheng , Sun Qing , Wang Fengfei , Wu Erxi 
Published: January 2013

Abstract
Pancreatic cancer significantly affects the quality of life due to the severe abdominal pain. However, the underlying mechanism is not clear. This study aimed to determine the relationship between Substance P (SP) and pancreatic cancer perineural invasion (PNI) as well as the mechanism of SP mediating pancreatic cancer PNI, which causes pain in patients with pancreatic cancer. Human pancreatic cancer cells and newborn dorsal root ganglions (DRG) were used to determine the expression of SP or NK-1R in pancreatic cancer cells and DRGs cells by QT-PCR and Western blotting. The effects of SP on pancreatic cancer cell proliferation and invasion were analyzed using MTT assay and Transwell Matrigel invasion assay, respectively. Alterations in the neurotropism of pancreatic cancer cells were assessed by coculture system, which mimics the interaction of tumor/neuron in vivo. SP is not only widely distributed in the neurite outgrowth from newborn DRGs but also expressed in MIA PaCa-2 and BxPC-3 cells. NK-1R is found to be overexpressed in the pancreatic cancer cell lines examined. SP induces cancer cell proliferation and invasion as well as the expression of matrix metalloproteinase (MMP)-2 in pancreatic cancer cells, and NK-1R antagonists inhibit these effects. Furthermore, SP promotes neurite outgrowth and the migration of pancreatic cancer cell cluster to the DRGs, which is blocked by NK-1R antagonists in the coculture model. Our results suggest that SP plays an important role in the development of pancreatic cancer metastasis and PNI, and blocking the SP/NK-1R signaling system is a novel strategy for the treatment of pancreatic cancer. Mol Cancer Res; 11(3); 294–302. ©2012 AACR.
Methicillin-resistant Staphylococcus aureus-induced thrombo-inflammatory response is reduced with timely antibiotic administration
Authors: de Abreu Adriana Vieira , Holloway Jeffrey T. , Marvin James E. , Kraemer Bjoern F. , Zimmerman Guy A. , Weyrich Andrew S. , Franks Zechariah , Campbell Robert A. , Rondina Matthew T. 
Published: January 2013

SummaryMethicillin-resistant Staphylococcus aureus (MRSA) induces a prothrombotic and pro-inflammatory milieu. Although timely antibiotic administration in MRSA sepsis may improve outcomes by arresting bacterial growth, the effects of antibiotics on mitigating injurious thrombo-inflammatory cellular responses remains unexplored. Using a newly developed human whole blood model and an in vivo mouse model of MRSA infection, we examined how antibiotics inhibit MRSA induced thrombo-inflammatory pathways. Human whole blood was inoculated with MRSA. Thrombin generation and inflammatory cytokine synthesis was measured in the presence or absence of linezolid and vancomycin. C57BL/6 mice were injected with MRSA and the effect of vancomycin administration was examined. MRSA accelerated thrombin generation in a time- and concentration-dependent manner and induced the release of cytokines, including interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1. The increase in thrombin generation and inflammatory responses was mediated through the synthesis of tissue factor and cytokines, respectively, and the release of microparticles. The early administration of antibiotics restored normal thrombin generation patterns and significantly reduced the synthesis of cytokines. In contrast, when antibiotic administration was delayed, thrombin generation and cytokine synthesis were not significantly reduced. In mice infected with MRSA, early antibiotic administration reduced thrombin anti-thrombin complexes and cytokine synthesis, whereas delayed antibiotic administration did not. These data provide novel mechanistic evidence of the importance of prompt antibiotic administration in infectious syndromes.
Interpretation of Left Ventricular Diastolic Dysfunction in Children With Cardiomyopathy by Echocardiography
Authors: Dragulescu Andreea , Mertens Luc , Friedberg Mark K. 
Published: January 2013

Background—
Left ventricular diastolic dysfunction (DD) is a key determinant of outcomes in pediatric cardiomyopathy (CM), but remains very challenging to diagnose and classify. Adult paradigms and guidelines relating to DD are currently applied in children. However, it is unknown whether these are applicable to children with CM. We investigated the assessment of DD in children with CM using adult and pediatric echocardiographic criteria and tested whether recent adult guidelines are applicable to this population.

Methods and Results—
Three investigators independently classified diastolic function in 4 study groups: controls, dilated, hypertrophic, and restrictive CM. Agreement among investigators, failure to classify DD, and the reasons for diagnostic failure were determined. The usefulness of individual echo parameters to diagnose and classify DD was assessed. One hundred seventy-five children (aged 0–18 years) were studied. DD diagnostic criteria were discrepant in the majority of patients. Delayed relaxation was diagnosed in only 14% of hypertrophic CM patients and never in dilated CM and restrictive CM, with 50% of those patients having coexisting findings of elevated filling pressures. Many key parameters, such as mitral and pulmonary venous Doppler, were not informative. Agreement among investigators for grading of DD was poor (36% of CM patients).

Conclusions—
Assessment of DD in childhood CM seems inadequate using current guidelines. The large range of normal pediatric reference values allows diagnosis of DD in only a small proportion of patients. Key echo parameters to assess DF are not sufficiently discriminatory in this population, and discrepancies between criteria within individuals prevent further classification and result in poor interobserver agreement.
Stepwise Screening of Atrial Fibrillation in a 75-Year-Old Population
Authors: Engdahl Johan , Andersson Lisbeth , Mirskaya Maria , Rosenqvist Mårten 
Published: January 2013

Background—
Atrial fibrillation (AF) is a frequent source of cardiac emboli in patients with ischemic stroke. AF may be asymptomatic and therefore undiagnosed. Screening for silent AF seems suitable in risk populations, however little is known on the yield and cost-effectiveness of such screening.

Methods and Results—

All inhabitants in the municipality of Halmstad, Sweden aged 75 to 76 years were invited to a stepwise screening program for AF. As a first step, participants recorded a 12-lead ECG and reported their relevant medical history. Those with sinus rhythm on 12-lead ECG, no history of AF, and ≥2 risk factors according to CHADS
2
were invited to a 2-week recording period using a hand-held ECG and asked to record 20 or 30 seconds twice daily and if palpitations occurred. One thousand, three hundred thirty inhabitants were invited, of whom 848 (64%) participated. Previously undiagnosed silent AF was found in 10 (1%) among 848 individuals who recorded 12-lead ECG. Among 81 patients with known AF, 35 (43%) were not on oral anticoagulation treatment. Among 403 persons with ≥2 risk factors for stroke, who completed the hand-held ECG event recording, 30 (7.4%) were diagnosed with paroxysmal AF. Thus 75/848 (9%) of the screened population were candidates for new oral anticoagulation treatment, of those 57 actually started oral anticoagulation treatment.

Conclusions—
Stepwise risk factor–stratified AF screening in a 75-year-old population yields a large share of candidates for oral anticoagulation treatment on AF indication.
Composition and structure of a snake assemblage in an altered tropical forest-plantation mosaic in Bangladesh
Authors: Rahman Shahriar Caesar , Rashid S.M.A. , Das Kanai , Luiselli Luca 
Published: January 2013

Despite the growing trends in quantitative field studies on tropical snake assemblages around the world, Asian tropical snake assemblages have remained less profoundly studied. A snake assemblage in an altered tropical forest-plantation mosaic in Bangladesh was studied for six months. Data were collected on the species composition and their relative frequency of occurrence. On the basis of these data, some major patterns highlighted by earlier studies on tropical snake ecology were tested. More specifically, we tested, the existence of: (1) non-random habitat niche partitioning, (2) the energetic equivalence rule, and (3) different mean body sizes among snake guilds, with distinctly smaller body sizes being expected among the subterranean species. A total of 374 specimens belonging to 34 different species were collected. High mean habitat niche overlap among species was observed, and there was no apparent non-random niche partitioning by snakes either considering all species together or dividing them by guild. The ‘energetic equivalence rule’ was verified, with larger species being less abundant than smaller species. Body sizes differed significantly across species’ habits, with subterranean species being not only significantly smaller but also revealing the least interspecific variation, and terrestrial/arboreal species showing the greatest interspecific variation. Overall, tropical Asian snake assemblages seem to be similar to tropical African snake assemblages in terms of their general organization.
An Online Means of Testing Asymmetries in Seating Preference Reveals a Bias for Airplanes and Theaters
Authors: Nicholls Michael E. R. , Thomas Nicole A. , Loetscher Tobias 
Published: January 2013

Objective: The aim of this study was to investigate asymmetrical interactions between humans and their environment using online seat booking sites. Background: Functional differences between the cerebral hemispheres affect the choices people make. For example, when asked to imagine going to a cinema, people preferentially select seats to the right. We investigated whether this experimental research generalizes to online booking sites for aircraft and theaters. Method: Occupancy rates for seats taken on the left and right sides were assessed for 100 airline flights with 12,762 available seats and 37 theater performances with 34,456 seats. On the basis of previous research, a rightward bias was predicted for aircraft and theaters. Results: For aircraft, contrary to expectation, occupancy rate was higher for left-compared with right-side seats. For theaters, a rightward bias was observed when the theater was less than half full. The bias was not affected by the orientation of the map. Conclusion: For aircraft, the leftward preference could be attributable to a rightward turning bias or a “feeling” that the port seats are closer to the exit, even though they are not. For theaters, the data demonstrate that the rightward preference observed in earlier studies exists only when the theater is relatively empty. Application: Asymmetrical seating may play an important role in the efficient assimilation of information from the environment, and this role should take this into account when designing effective human–environment interfaces. The online method of assessing seating used in the current study provides an informative and potentially powerful means of assessing asymmetries in human perception and action.
Effects of acute systemic administration of cannabidiol on sleep-wake cycle in rats
Authors: Chagas Marcos Hortes N , Crippa José Alexandre S , Zuardi Antonio Waldo , Hallak Jaime E C , Machado-de-Sousa João Paulo , Hirotsu Camila , Maia Lucas , Tufik Sergio , Andersen Monica Levy 
Published: January 2013

Cannabidiol (CBD) is one of the main components of Cannabis sativa and has a wide spectrum of action, including effects in the sleep-wake cycle. Objective: The objective of this paper is to assess the effects on sleep of acute systemic administration of CBD. Method: Adult male Wistar rats were randomly distributed into four groups that received intraperitoneal injections of CBD 2.5 mg/kg, CBD 10 mg/kg, CBD 40 mg/kg or vehicle ( n=seven animals/group). Sleep recordings were made during light and dark periods for four days: two days of baseline recording, one day of drug administration (test), and one day after drug (post-test). Results: During the light period of the test day, the total percentage of sleep significantly increased in the groups treated with 10 and 40 mg/kg of CBD compared to placebo. REM sleep latency increased in the group injected with CBD 40 mg/kg and was significantly decreased with the dose of 10 mg/kg on the post-test day. There was an increase in the time of SWS in the group treated with CBD 40 mg/kg, although this result did not reach statistical significance. Conclusion: The systemic acute administration of CBD appears to increase total sleep time, in addition to increasing sleep latency in the light period of the day of administration.
Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests
Authors: Becker Sören L , Vogt Jürg , Knopp Stefanie , Panning Marcus , Warhurst David C , Polman Katja , Marti Hanspeter , von Müller Lutz , Yansouni Cedric P , Jacobs Jan , Bottieau Emmanuel , Sacko Moussa , Rijal Suman , Meyanti Fransiska , Miles Michael A , Boelaert Marleen , Lutumba Pascal , van Lieshout Lisette , N’Goran Eliézer K , Chappuis François , Utzinger Jürg 
Published: January 2013

Abstract

Background
Persistent digestive disorders account for considerable disease burden in the tropics. Despite advances in understanding acute gastrointestinal infections, important issues concerning epidemiology, diagnosis, treatment and control of most persistent digestive symptomatologies remain to be elucidated. Helminths and intestinal protozoa are considered to play major roles, but the full extent of the aetiologic spectrum is still unclear. We provide an overview of pathogens causing digestive disorders in the tropics and evaluate available reference tests.

Methods
We searched the literature to identify pathogens that might give rise to persistent diarrhoea, chronic abdominal pain and/or blood in the stool. We reviewed existing laboratory diagnostic methods for each pathogen and stratified them by (i) microscopy; (ii) culture techniques; (iii) immunological tests; and (iv) molecular methods. Pathogen-specific reference tests providing highest diagnostic accuracy are described in greater detail.

Results
Over 30 pathogens may cause persistent digestive disorders. Bacteria, viruses and parasites are important aetiologic agents of acute and long-lasting symptomatologies. An integrated approach, consisting of stool culture, microscopy and/or specific immunological techniques for toxin, antigen and antibody detection, is required for accurate diagnosis of bacteria and parasites. Molecular techniques are essential for sensitive diagnosis of many viruses, bacteria and intestinal protozoa, and are increasingly utilised as adjuncts for helminth identification.

Conclusions
Diagnosis of the broad spectrum of intestinal pathogens is often cumbersome. There is a need for rapid diagnostic tests that are simple and affordable for resource-constrained settings, so that the management of patients suffering from persistent digestive disorders can be improved.
The antioxidant response of the liver of male Swiss mice raised on a AIN 93 or commercial diet
Authors: Caetano Aline C , da Veiga Lucimara F , Capaldi Flávia R , de Alencar Severino M , Azevedo Ricardo A , Bezerra Rosangela MN 
Published: January 2013

Abstract

Background
Reactive oxygen species (ROS) are formed under natural physiological conditions and are thought to play an important role in many human diseases. A wide range of antioxidants are involved in cellular defense mechanisms against ROS, which can be generated in excess during stressful conditions, these include enzymes and non-enzymatic antioxidants. The aim of this study was to evaluate the antioxidant responses of mice to two diets control, commercial and the purified AIN 93 diet, commonly used in experiments with rodents.

Results
Malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations and superoxide dismutase (SOD) and glutathione reductase (GR) activities determined in the liver were lower in the group of mice fed with the AIN 93 diet, while catalase (CAT) activity was higher in the same group, when compared to the group fed on the commercial diet. Liver glutathione peroxidase (GSH-Px) activity was similar in the groups fed on either AIN 93 or the commercial diets. Two SOD isoforms, Mn-SODII and a Cu/Zn-SODV, were specifically reduced in the liver of the AIN 93 diet fed animals.

Conclusions
The clear differences in antioxidant responses observed in the livers of mice fed on the two diets suggest that the macro- and micro-nutrient components with antioxidant properties, including vitamin E, can promote changes in the activity of enzymes involved in the removal of the ROS generated by cell metabolism.