bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of reactive oxygen species generation inhibits epithelial–mesenchymal transition and promotes growth arrest in prostate cancer cells
Authors: Das Trinath P. , Suman Suman , Damodaran Chendil 
Published: March 2013

AbstractOxidative stress is one causative factor of the pathogenesis and aggressiveness of most of the cancer types, including prostate cancer (CaP). A moderate increase in reactive oxygen species (ROS) induces cell proliferation whereas excessive amounts of ROS promote apoptosis. In this study, we explored the pro‐oxidant property of 3,9‐dihydroxy‐2‐prenylcoumestan (psoralidin [pso]), a dietary agent, on CaP (PC‐3 and C4‐2B) cells. Pso greatly induced ROS generation (more than 20‐fold) that resulted in the growth inhibition of CaP cells. Overexpression of anti‐oxidant enzymes superoxide dismutase 1 (SOD1), SOD2, and catalase, or pretreatment with the pharmacological inhibitor N‐acetylcysteine (NAC) significantly attenuated both pso‐mediated ROS generation and pso‐mediated growth inhibition in CaP cells. Furthermore, pso administration significantly inhibited the migratory and invasive property of CaP cells by decreasing the transcription of β‐catenin, and slug, which promote epithelial–mesenchymal transition (EMT), and by concurrently inducing E‐cadherin expression in CaP cells. Pso‐induced ROS generation in CaP cells resulted in loss of mitochondrial membrane potential, cytochrome‐c release, and activation of caspase‐3 and ‐9 and poly (ADP‐ribose) polymerase (PARP), which led to apoptosis. On the other hand, overexpression of anti‐oxidants rescued pso‐mediated effects on CaP cells. These findings suggest that increasing the threshold of intracellular ROS could prevent or treat CaP growth and metastasis. © 2013 Wiley Periodicals, Inc.
ProFit revisited
Authors: Fuchs Alexander , Boesiger Peter , Schulte Rolf F. , Henning Anke 
Published: March 2013

PurposeAn enhanced version of the ProFit fitting tool was developed and validated to improve the quantification of two‐dimensional JRPESS spectroscopic data.MethodsThe proposed enhancements were achieved by flexible organization of prior knowledge, configurations for different situations, the inclusion of measured macromolecular baseline contribution, additional baseline splines and a model‐free lineshape based on self‐deconvolution. The new software was tested and tuned on simulated data and subsequently applied to in vivo intrasubject and intersubject data.ResultsFit results of simulated and acquired spectra show good overall quality suggesting the potential reliable detection of up to 18 metabolites on a 3T system yielding Cramer‐Lower‐Bounds below 20%.ConclusionThe proposed enhanced version of ProFit together with two‐dimensional J‐resolved spectroscopy offers the opportunity to reliably detect a wide selection of important brain metabolites on 3T. Magn Reson Med 71:458–468, 2014. © 2013 Wiley Periodicals, Inc.
Uniform spinning sampling gradient electron paramagnetic resonance imaging
Authors: Johnson David H. , Ahmad Rizwan , Liu Yangping , Chen Zhiyu , Samouilov Alexandre , Zweier Jay L. 
Published: March 2013

PurposeTo improve the quality and speed of electron paramagnetic resonance imaging (EPRI) acquisition by combining a uniform sampling distribution with spinning gradient acquisition.Theory and MethodsA uniform sampling distribution was derived for spinning gradient EPRI acquisition (uniform spinning sampling, USS) and compared to the existing (equilinear spinning sampling, ESS) acquisition strategy. Novel corrections were introduced to reduce artifacts in experimental data.ResultsSimulations demonstrated that USS puts an equal number of projections near each axis whereas ESS puts excessive projections at one axis, wasting acquisition time. Artifact corrections added to the magnetic gradient waveforms reduced noise and correlation between projections. USS images had higher SNR (85.9 ± 0.8 vs. 56.2 ± 0.8) and lower mean‐squared error than ESS images. The quality of the USS images did not vary with the magnetic gradient orientation, in contrast to ESS images. The quality of rat heart images was improved using USS compared to that with ESS or traditional fast‐scan acquisitions.ConclusionA novel EPRI acquisition which combines spinning gradient acquisition with a uniform sampling distribution was developed. This USS spinning gradient acquisition offers superior SNR and reduced artifacts compared to prior methods enabling potential improvements in speed and quality of EPR imaging in biological applications. Magn Reson Med 71:893–900, 2014. © 2013 Wiley Periodicals, Inc.
Brain responses to bladder filling in older women without urgency incontinence
Authors: Tadic Stasa D. , Tannenbaum Cara , Resnick Neil M. , Griffiths Derek 
Published: March 2013

AbstractAimsTo investigate normal brain responses to bladder filling, especially when there is little or no sensation as in much of daily life.MethodsWe performed an functional magnetic resonance imaging (fMRI) study of brain responses to bladder filling in normal female subjects, evoked by infusion and withdrawal of fluid in and out of the bladder. Using the contrast (infusion–withdrawal), we imaged brain activity at small bladder volumes with weak filling sensation and also with full bladder and strong desire to void.ResultsEleven women, average age 65 years (range: 60–71 years) were included. With full bladder and strong desire to void, filling provoked a well‐known pattern of activation near the right insula and (as a trend) in the dorsal anterior cingulate cortex and supplementary motor area. There was no significant deactivation. With small bladder volume filling provoked widespread apparent deactivation and no significant activation. Apparent deactivation was associated with increased fMRI signal during withdrawal rather than decrease during infusion, suggesting artifact. A correction for global changes in cerebral blood flow eliminated it and revealed significant subcortical activation, although none in frontal or parietal cortex.ConclusionsIn older women with normal bladder function, infusion into an already full bladder resulted in strong sensation and brain activation near the insula and in the dorsal anterior cingulate/supplementary motor complex. With near‐empty bladder and little sensation, the situation during much of daily life, these cortical areas were not detectably activated, but activation in midbrain and parahippocampal regions presumably indicated unconscious monitoring of ascending bladder signals. Neurourol. Urodynam. 32: 435–440, 2013. © 2013 Wiley Periodicals, Inc.
Functionalization of porous agarose film with single‐walled carbon nanotubes as excellent electrochemical interface materials
Authors: Li Ru , Li Hongbo , Zhang Jing , Jin Hehua , Li Qingwen 
Published: March 2013

AbstractThree‐dimensional (3D) agarose gel network could be readily modified with single‐walled carbon nanotubes (SWNTs) through their noncovalent interactions. Because of the embedded SWNT network, the agarose‐SWNT film fabricated by the gel‐drying method well maintained its in‐plane shape and size. Compared with pure agarose film, the maximum strain at break and strength of agarose‐SWNT film could be increased by 67.2% and 24%, respectively. More importantly, modifying the bare glass carbon electrode (GCE) with agarose‐SWNT film, we observed 12 times increase in dopamine (DA) electrochemical peak current, and 2.6 times increase in the case of potassium ferricyanide (K3Fe(CN)6). The porous 3D agarose network interpenetrated with high‐conductivity SWNT network might contribute to the excellent electrochemical interface property. POLYM. COMPOS., 2013. © 2013 Society of Plastics Engineers
Epoxy composites based on inexpensive char filler obtained from plastic waste and natural resources
Authors: Ahmetli Gulnare , Kocaman Suheyla , Ozaytekin Ilkay , Bozkurt Pınar 
Published: March 2013

AbstractIn this study, plastic [polyethylene terephthalate (PET)] waste was recycled as raw material for the preparation of diglycidyl ether of bisphenol A‐type epoxy composite materials. The other inexpensive fillers used to prepare the composites were wood shavings char and pine cone char (PCC), obtained from natural resources. The thermogravimetric analysis showed that plastic waste char (PWC) and PCC can significantly improve the thermal stability of neat epoxy resin at temperatures above 300°C. The best thermal and electrical conductivity results were obtained with PWC. The residual weight of the composite with 30 wt% PWC was 69%. Surface hardness, Young's modulus, and tensile strength of the composites were higher than those with a pure epoxy polymer matrix. The composite morphology was characterized by X‐ray diffraction and scanning electron microscopy. POLYM. COMPOS., 2013. © 2013 Society of Plastics Engineers
Characterization of UHMWPE/wood composites produced via dry‐blending and compression molding
Authors: Mahfoudh A. , Cloutier A. , Rodrigue D. 
Published: March 2013

AbstractIn this study, composites made from wood flour and ultra‐high molecular weight polyethylene (UHMWPE) were produced and characterized. In particular, the composites were initially prepared using a simple dry mixing technique and then compression molded. The effect of wood content on the mechanical properties was determined up to 30wt%. Characterization included scanning electron microscopy to investigate wood dispersion and interfacial bonding quality, while the mechanical tests included tensile, torsion, and flexion. The results show that good dispersion and adhesion was achieved and wood flour addition increased substantially (up to 97%) all the moduli in the range of conditions tested. Finally, it was found that hardness increased by about 5 Shore D points by adding 30% wood flour in UHMWPE. POLYM. COMPOS., 2013. © 2013 Society of Plastics Engineers
Vital Exhaustion and Markers of Low‐Grade Inflammation in Healthy Adults: The Amsterdam Growth and Health Longitudinal Study
Authors: Hoekstra Trynke , Barbosa‐Leiker Celestina , Twisk Jos W.R. 
Published: March 2013

AbstractThis study analyses distinct trajectories of vital exhaustion (VE; a measure of mental health incorporating signs of stress and depression) over a period of 15 years in healthy adults and investigates further the consequences for markers of low‐grade inflammation as indicators of cardiovascular disease risk. Data of 341 participants of the Amsterdam Growth and Health Longitudinal Study were utilized. VE was measured by the Maastricht Questionnaire. Markers of low‐grade inflammation included interleukin‐6, interleukin‐8 and tumour necrosis factor‐α. Distinct trajectories of VE were obtained by latent class growth models, and consequences for markers of low‐grade inflammation of the trajectories were analysed by linear regressions. We found comparable trajectories of VE for men and women; a ‘never vitally exhausted’ subgroup (16.9% and 25.1%, respectively), a ‘stable preclinical VE’ subgroup (51.7% and 68.1%) and a ‘chronic VE state’ subgroup (31.5% and 6.7%). The subgroups had similar levels of the markers investigated. This study is the first to analyse VE longitudinally in healthy adults and indicates that although distinct trajectories of VE were identified, differential consequences for cardiovascular disease risk were unapparent. Copyright © 2013 John Wiley & Sons, Ltd.
Surface‐Mediated Nucleic Acid Delivery by Lipoplexes Prepared in Microwell Arrays
Authors: Wu Yun , Terp Megan Cavanaugh , Kwak Kwang Joo , Gallego‐Perez Daniel , Nana‐Sinkam Serge P. , Lee L. James 
Published: March 2013

AbstractMany delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acid‐based therapeutics. A facile surface‐mediated nucleic acid delivery by lipoplexes is prepared in a microwell array, which combines the advantages of lipoplexes as an efficient carrier system, surface‐mediated delivery, and the control of surface topography. Uniform disc‐like lipoplexes containing nucleic acids are formed in the microwell array with a diameter of ∼818 nm and thickness of ∼195 nm. The microwell array‐mediated delivery of lipoplexes containing FAM‐oligodeoxynucleotides is ∼18.6 and ∼10.6 times more efficient than the conventional transfection method in an adherent cell line (A549 non‐small cell lung cancer cells) and a suspension cell line (KG‐1a acute myelogenous leukemia cells), respectively. MicroRNA‐29b is then used as a model nucleic acid to investigate the therapeutic efficacy of lipoplexes delivered by the microwell array. Compared to conventional transfection methods, the effective therapeutic dosage of microRNA‐29b is reduced from the microgram level to the nanogram level by lipoplexes prepared in the microwell array. The microwell array is also a very flexible platform. Both nucleic acid therapeutics and imaging reagents are incorporated in lipoplexes and successfully delivered to A549 cells, demonstrating its potential applications in theranostic medicine.
An adaptive moving finite volume scheme for modeling flood inundation over dry and complex topography
Authors: Zhou Feng , Chen Guoxian , Huang Yuefei , Yang Jerry Zhijian , Feng Hui 
Published: March 2013

Key Points
AMFV is well‐balanced positivity preserving for modeling flood inundation
Shock‐capturing capability is improved over dry and complex topography
Predictive accuracy and spatial resolution is improved with fewer meshes
Long‐term trends in evapotranspiration and runoff over the drainage basins of the Gulf of Mexico during 1901–2008
Authors: Liu Mingliang , Tian Hanqin , Yang Qichun , Yang Jia , Song Xia , Lohrenz Steven E. , Cai Wei‐Jun 
Published: March 2013

Key Points
Significant regional differences in ET and R trends are found.
Land use change, as well as climate change, affects trends of water fluxes
None significant long‐term trends in freshwater discharge to the GOM is found
Laparoscopic Treatment for Unsuspected Common Bile Duct Stones by Transcystic Sphincter of Oddi Pneumatic Balloon Dilation and Pressure‐Washing Technique
Authors: Masoni Luigi , Mari Francesco Saverio , Pietropaolo Vincenzo , Onorato Maurizio , Meucci Massimo , Brescia Antonio 
Published: March 2013

AbstractBackgroundUnsuspected common bile duct stones (CBDS) are found in 4–5 % of patients with cholelithiasis. The optimal strategy for the treatment of asymptomatic CBDS, diagnosed during laparoscopic cholecystectomy (LC), is not yet well established. A one‐stage solution is preferable to solve the CBDS during the LC and to avoid the exposure of patients to the risks of a second procedure, such as complications or failure.MethodsWe attempted to remove CBDS by transcystic sphincter of Oddi pneumatic balloon dilation and common bile duct pressure‐washing in all cases of intraoperative identification of CBDS since September 2008.ResultsIn 29 cases, unsuspected CBDS was identified by intraoperative cholangiography; in 28 cases a single stone with a mean diameter of 4.3 mm (range = 3–6) was detected and in one case three 5–8‐mm‐diameter stones were identified. Clearance of the common bile duct was obtained in 27 cases (96 %), with a mean operative time of 54 min (range = 36–90) and mean length of hospital stay of 2.5 days.ConclusionTreatment of unsuspected CBDS detected by intraoperative cholangiography during LC with this original technique was safe and effective and a viable alternative of the transcystic endoscopic approach.
Aggregation Behavior of Perfluorononanoic Acid/Sodium Dodecyl Sulfate Mixtures
Authors: Torres María Florencia , de Rossi Rita H. , Fernández Mariana Adela 
Published: March 2013

AbstractThe mixed system of an anionic hydrocarbon surfactant, sodium dodecyl sulfate, and a perfluorinated surfactant, perfluorononanoic acid (PFNA), was investigated by a combination of methods. The critical micellar concentrations (CMC) were determined over a wide range of sample compositions by surface tension, conductivity and UV–visible spectrophotometry using N‐(4‐nitrophenyl)perfluorononanamide as a molecular probe. The values of CMC obtained by different techniques were in good agreement. In addition, the aggregation numbers were determined in the mixtures with a low content of hydrocarbon surfactant, by measuring the fluorescence quenching of pyrene. The hydrodynamic radii of the aggregates were estimated through the diffusion ordered 19F‐ and 1H‐NMR spectroscopy. The values obtained are in agreement with those expected according to the measured aggregation numbers. The analysis of the data with different aggregation models suggests the formation of a non‐ideal mixed micelle that is enriched in the perfluorinated surfactant when its mole fraction increases, and that is practically formed by PFNA only at mole fractions higher than 0.8.
Effects of Surfactant Hydrophilicity on the Oil Solubilization and Rheological Behavior of a Nonionic Hexagonal Phase
Authors: Alam Mohammad Mydul , Matsumoto Yohei , Aramaki Kenji 
Published: March 2013

AbstractThe effects of surfactant hydrophilicity on the phase behavior and rheology of a hexagonal phase (H1) and related O/H1 emulsions in a water/C12EOn/isohexadecane system (n = 7, 9) have been investigated. In this phase behavior study, a variety of surfactant aggregates (micellar, Wm; hexagonal, H1; bicontinuous cubic, V1; lamellar, Lα; inverse micellar, Om) have been observed in water‐C12EOn binary systems. The oil solubility of different surfactant aggregates was strongly affected by the surfactant hydrophilicity, i.e., surfactant aggregates in the C12EO7 system could solubilize a larger amount of oil than those in the C12EO9 system. It can be inferred that the surfactant with long hydrophilic head groups could be closely packed in the interface, leading to a reduction in the oil solubility in the micellar core due to the strong head group interaction. Interestingly, the rheology of the H1 phase also exhibits behavior that is dependent on the surfactant hydrophilicity, i.e., high values of the elastic modulus G′ and complex viscosity |η*| have been observed in the C12EO9 system, possibly due to the strong neighboring head group interaction. Hexagon structure of the H1 phase was calculated by using the Bohlin model. The viscosity of the O/H1 emulsions decreases with increasing oil concentration, possibly due to the low volume fraction of the continuous phase (the H1 phase).
Pro‐Oxidant Effects of β‐Carotene During Thermal Oxidation of Edible Oils
Authors: Zeb Alam , Murkovic Michael 
Published: March 2013

Abstractβ‐Carotene is one of the most important fat soluble pigments with well‐known antioxidant and pro‐vitamin A activity. It is used in industries as a food colorant and a source of vitamin A. The thermal induced degradation during processing of wide varieties of carotenoid‐rich foods leads to color and properties losses. The thermal stability of edible oils is thus of great importance to food manufacturers. Corn oil, rapeseed, and sunflower oils were fortified with 50–300 μg/g of β‐carotene and oxidized using a Rancimat apparatus (air flow rate 20 L/h) at 110 °C for 14 h. β‐Carotene degradation was measured using high performance thin layer chromatography and confirmed by HPLC–DAD–MS. Triacylglycerols and polar compounds (PC) were determined using LC–ESI–MS. Results showed that most of the β‐carotene was degraded during the first 5 h of the thermal oxidation. It was found that the addition of β‐carotene produces significant effects (P < 0.05) on the peroxide index, free fatty acid values and radical scavenging activity of the three oils. Triacylglycerols containing high amounts of oleic acid show higher stability toward thermal oxidation and β‐carotene treatment. Among the oils, rapeseed oil was the most stable oil in terms of the formation of polar compounds (PC), followed by corn oil, while sunflower oil was more prone to oxidation and thus higher amounts of PC were formed.
Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells
Authors: Vedantam Pallavi , Huang George , Tzeng T. R. Jeremy 
Published: March 2013

AbstractUrinary tract infection (UTI) is a predominant condition in prostate cancer patients.Escherichia coliORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.
Connectomics: novel methods for the dense reconstruction of neuronal circuits
Authors: Helmstaedter M. 
Published: March 2013

Abstract
The nervous system is characterized by ex­tremely complex cell-to-cell interactions which primarily occur via chemical synaps­es. Mapping the structure of these intercel­lular networks is one of the major challeng­es in neuroscience. The new field of connec­tomics which has developed in recent years aims at the dense reconstruction of increas­ingly comprehensive nerve cell networks. Au­tomated volume electron microscopy tech­niques are employed for image acquisition. A major obstacle, however, is data reconstruc­tion, for which unusual solutions (e.g., mass reconstruction by crowd sourcing and on­line computer games) are currently being pursued.
CONSTRUCTIONS BY RULER AND COMPASS, TOGETHER WITH A FIXED CONIC
Authors: BAEK SEUNGJIN , CHOE INSONG , JUNG YOONHO , LEE DONGWOOK , SEO JUNGGYO 
Published: March 2013

AbstractIt is well known to be impossible to trisect an arbitrary angle and duplicate an arbitrary cube by a ruler and a compass. On the other hand, it is known from ancient times that these constructions can be performed when the use of several conic curves is allowed. In this paper, we prove that any point constructible from conics can be constructed using a ruler and a compass, together with a single fixed nondegenerate conic different from a circle.
TWO QUESTIONS OF L. VAŠ ON -CLEAN RINGS
Authors: CHEN JIANLONG , CUI JIAN 
Published: March 2013

AbstractA $\ast $-ring $R$ is called (strongly) $\ast $-clean if every element of $R$ is the sum of a unit and a projection (that commute). Vaš [‘$\ast $-Clean rings; some clean and almost clean Baer $\ast $-rings and von Neumann algebras’, J. Algebra 324(12) (2010), 3388–3400] asked whether there exists a $\ast $-ring that is clean but not $\ast $-clean and whether a unit regular and $\ast $-regular ring is strongly $\ast $-clean. In this paper, we answer these two questions. We also give some characterisations related to $\ast $-regular rings.
GROUPOID -ALGEBRAS WITH HAUSDORFF SPECTRUM
Authors: GOEHLE GEOFF 
Published: March 2013

AbstractSuppose that $G$ is a second countable, locally compact Hausdorff groupoid with abelian stabiliser subgroups and a Haar system. We provide necessary and sufficient conditions for the groupoid ${C}^{\ast } $-algebra to have Hausdorff spectrum. In particular, we show that the spectrum of ${C}^{\ast } (G)$ is Hausdorff if and only if the stabilisers vary continuously with respect to the Fell topology, the orbit space ${G}^{(0)} / G$ is Hausdorff, and, given convergent sequences ${\chi }_{i} \rightarrow \chi $ and ${\gamma }_{i} \cdot {\chi }_{i} \rightarrow \omega $ in the dual stabiliser groupoid $\widehat{S}$ where the ${\gamma }_{i} \in G$ act via conjugation, if $\chi $ and $\omega $ are elements of the same fibre then $\chi = \omega $.