bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ambivalence in Narrative Therapy: A Comparison Between Recovered and Unchanged Cases
Authors: Ribeiro António P. , Gonçalves Miguel M. , Silva Joana R. , Brás Andreia , Sousa Ines 
Published: March 2015

Research on the identification of poor outcome predictors is crucial for the prevention of therapeutic failure. Previous research suggests that clients' persistent ambivalence is one possible path to unsuccessful psychotherapy. The present study analyses ambivalence—here operationalized as return‐to‐the‐problem markers (RPMs)—in five recovered and five unchanged cases of narrative psychotherapy for major depression. The results suggest that both recovered and unchanged cases presented a similar proportion of RPMs at baseline and a decreasing pattern of these ambivalence markers throughout therapy. However, the decreasing was more accentuated in recovered than in unchanged cases, and at the end of the treatment, the proportion of RPMs of the unchanged cases was significantly higher. The results are discussed in light of previous research on ambivalence in psychotherapy, focusing on the meaning of ambivalence and its clinical implications. Copyright © 2015 John Wiley & Sons, Ltd.Key Practitioner Message

Ambivalence towards change, here operationalized as RPMs, seems to be a common process in both recovered and unchanged cases, perhaps signalling the uncertainty and anxiety that change may elicit.
Although the number of RPMs decreased in both the recovered and unchanged cases, this reduction was significantly higher in the recovered group. Moreover, at the end of therapy, the recovered group revealed a significantly lower proportion of RPMs than the unchanged group, suggesting that ambivalence resolution (or lack thereof) may play a determining role in the therapy's evolution and outcome.
RPMs in later stages of therapy may be operationalized as ‘red flags’ for the therapist to acknowledge the client's stuckness and adapt his or her intervention efforts, turning these instances into developmental opportunities.
The Effects of Climate Change Policy on the Business Community: A Corporate Environmental Accounting Perspective
Authors: Evangelinos Konstantinos , Nikolaou Ioannis , Leal Filho Walter 
Published: March 2015

AbstractThis paper proposes a normative framework to explain the influence of climate change policy on corporate environmental strategies, and at the same time describes how these strategies could be translated into an integrated environmental accounting model based on formal accounting statements. In particular, it aims to demonstrate a new approach of climate change accounting in order to overcome some important weaknesses of previous environmental accounting methods, such as accuracy and reliability. Currently, the majority of existing accounting methods record information on climate change on a voluntary basis. Even though they may assist businesses in designing essential environmental strategies and help public policy entities improve their knowledge on the results of such strategies, the informal nature of the accounting standards and the variable type of financial and non‐financial information make the use of such models untrustworthy and complicated. Finally, an empirical example of this framework is also presented. Copyright © 2015 John Wiley & Sons, Ltd and ERP Environment.
prdm1a functions upstream of itga5 in zebrafish craniofacial development
Authors: LaMonica Kristi , Ding Hai‐lei , Artinger Kristin Bruk 
Published: March 2015

SummaryCranial neural crest cells are specified and migrate into the pharyngeal arches where they subsequently interact with the surrounding environment. Signaling and transcription factors, such as prdm1a regulate this interaction, but it remains unclear which specific factors are required for posterior pharyngeal arch development. Previous analysis suggests that prdm1a is required for posterior ceratobranchial cartilages in zebrafish and microarray analysis between wildtype and prdm1a mutants at 25 h post fertilization demonstrated that integrin α5 (itga5) is differentially expressed in prdm1a mutants. Here, we further investigate the interaction between prdm1a and itga5 in zebrafish craniofacial development. In situ hybridization for itga5 demonstrates that expression of itga5 is decreased in prdm1a mutants between 18 and 31 h post fertilization and itga5 expression overlaps with prdm1a in the posterior arches, suggesting a temporal window for interaction. Double mutants for prdm1a;itga5 have an additive viscerocranium phenotype more similar to prdm1a mutants, suggesting that prdm1a acts upstream of itga5. Consistent with this, loss of posterior pharyngeal arch expression of dlx2a, ceratobranchial cartilages 2–5, and cell proliferation in prdm1a mutants can be rescued with itga5 mRNA injection. Taken together, these data suggest that prdm1a acts upstream of itga5 and are both necessary for posterior pharyngeal arch development in zebrafish. genesis 53:270–277, 2015. © 2015 Wiley Periodicals, Inc.
Limited connectivity and a phylogeographic break characterize populations of the pink anemonefish, Amphiprion perideraion, in the Indo‐Malay Archipelago: inferences from a mitochondrial and microsatellite loci
Authors: Dohna Tina A. , Timm Janne , Hamid Lemia , Kochzius Marc 
Published: March 2015

AbstractTo enhance the understanding of larval dispersal in marine organisms, species with a sedentary adult stage and a pelagic larval phase of known duration constitute ideal candidates, because inferences can be made about the role of larval dispersal in population connectivity. Members of the immensely diverse marine fauna of the Indo‐Malay Archipelago are of particular importance in this respect, as biodiversity conservation is becoming a large concern in this region. In this study, the genetic population structure of the pink anemonefish, Amphiprion perideraion, is analyzed by applying 10 microsatellite loci as well as sequences of the mitochondrial control region to also allow for a direct comparison of marker‐derived results. Both marker systems detected a strong overall genetic structure (ΦST = 0.096, P < 0.0001; mean Dest = 0.17; FST = 0.015, P < 0.0001) and best supported regional groupings (ΦCT = 0.199 P < 0.0001; FCT = 0.018, P < 0.001) that suggested a differentiation of the Java Sea population from the rest of the archipelago. Differentiation of a New Guinea group was confirmed by both markers, but disagreed over the affinity of populations from west New Guinea. Mitochondrial data suggest higher connectivity among populations with fewer signals of regional substructure than microsatellite data. Considering the homogenizing effect of only a few migrants per generation on genetic differentiation between populations, marker‐specific results have important implications for conservation efforts concerning this and similar species.
Exogenous selection rather than cytonuclear incompatibilities shapes asymmetrical fitness of reciprocalArabidopsishybrids
Authors: Muir Graham , Ruiz‐Duarte Paola , Hohmann Nora , Mable Barbara K. , Novikova Polina , Schmickl Roswitha , Guggisberg Alessia , Koch Marcus A. 
Published: March 2015

AbstractReciprocal crosses between species often display an asymmetry in the fitness of F1hybrids. This pattern, referred to as isolation asymmetry or Darwin's corollary to Haldane's rule, is a general feature of reproductive isolation in plants, yet factors determining its magnitude and direction remain unclear. We evaluated reciprocal species crosses between two naturally hybridizing diploid species ofArabidopsisto assess the degree of isolation asymmetry at different postmating life stages. We found that pollen fromArabidopsis arenosawill usually fertilize ovules fromArabidopsis lyrata; the reverse receptivity being less complete. MaternalA. lyrataparents set more F1hybrid seed, but germinate at lower frequency, reversing the asymmetry. As predicted by theory,A. lyrata(the maternal parent with lower seed viability in crosses) exhibited accelerated chloroplast evolution, indicating that cytonuclear incompatibilities may play a role in reproductive isolation. However, this direction of asymmetrical reproductive isolation is not replicated in natural suture zones, where delayed hybrid breakdown of fertility at later developmental stages, or later‐acting selection againstA. arenosamaternal hybrids (unrelated to hybrid fertility, e.g., substrate adaptation) may be responsible for an excess ofA. lyratamaternal hybrids. Exogenous selection rather than cytonuclear incompatibilities thus shapes the asymmetrical postmating isolation in nature.
Construing multiple ingroups: Assessing social identity inclusiveness and structure in ethnic and religious minority group members
Authors: van Dommelen Andrea , Schmid Katharina , Hewstone Miles , Gonsalkorale Karen , Brewer Marilynn 
Published: March 2015

AbstractThe combination of multiple social identities into a coherent ingroup construal is of immediate relevance in today's complex and diverse societies. This paper proposes a conceptual and operational framework to examine how individuals subjectively construe their ingroup in the context of multiple, cross‐cutting group memberships. The subjective combination of multiple social identities is described in terms of structure (social identity structure) and inclusiveness (social identity inclusiveness (SII)). Two studies assess SII and social identity structure in community samples to whom the subjective combination of multiple, cross‐cutting ingroups is of particular relevance: a sample of Turkish‐Belgian Muslims (Study 1) and Turkish‐Australian Muslims (Study 2). Across both studies, SII uniquely predicted attitudes toward a range of outgroups, over and above identification with singular ingroups. Moreover, a wide range of social identity structures were identified, further attesting to broad individual differences in the construal of the perceived ingroup. Copyright © 2015 John Wiley & Sons, Ltd.
Electrochemical High Concentration Oxygen Sensing Using a Phosphonium Cation Based Room Temperature Ionic Liquid: Analytical Studies
Authors: Li Peilin , Compton Richard G. 
Published: March 2015

AbstractDetecting oxygen at the high concentration limit needs innovation. Room temperature ionic liquid trihexyl(tetradecyl)phosphonium trifluorotris(pentafluoroethyl)phosphate [P6,6,6,14][FAP] was tested as a solvent for oxygen detection through the electrochemical reduction of oxygen. Cyclic voltammetry of oxygen in nitrogen or carbon dioxide mixtures at different partial pressures was studied. The steady state current was shown to be linearly dependent on the oxygen partial pressure in high oxygen content (>77 %) systems. Simultaneous detecting of oxygen and carbon dioxide is also realised in an oxygen/carbon dioxide mixture, providing proof‐of‐concept of high concentration oxygen and carbon dioxide dual sensing.
Doxorubicin interactions with bovine serum albumin revealed by microdialysis with on‐line laser‐induced fluorescence detection at subpicogram level
Authors: Blazkova Iva , Smerkova Kristyna , Blazkova Lucie , Vaculovicova Marketa , Stiborova Marie , Eckschlager Tomas , Beklova Miroslava , Adam Vojtech , Kizek Rene 
Published: March 2015

Doxorubicin (DOX) is an effective antitumor drug employed for treatment of a wide range of cancers types such as neuroblastoma, osteosarcoma, breast and esophageal carcinomas. On the other hand, the cumulative dose is restricted (300–550 mg/m2) and its amount administered to a patient has to be closely controlled due to its cardiotoxicity. To understand the mechanisms of the DOX side effects as well as to reveal the ways how to reduce its adverse impact on cardiomyocytes, the interactions with particular components of the blood and tissues have to be studied in greater detail. In this work, microdialysis technique was optimized to extract DOX from samples and subsequently monitor its interaction with BSA. Finally, the microdialysis probe was connected on‐line to the LIF detector to ensure the real‐time detection. The best flow rate was 1 μL/min and after 120 min of microdialysis 28% of the DOX was dialyzed out from the sample. The results from investigation of the DOX–BSA interaction indicate that the interaction occurs in less than 30 min, causing marked decrease in the amount of DOX extracted by microdialysis.
Oil refinery experience with the assessment of refinery effluents and receiving waters using biologically based methods
Authors: Comber Michael H I , Girling Andrew , den Haan Klaas H , Whale Graham 
Published: March 2015

Abstract
The trend in discharges of petroleum-related substances from refineries in Europe shows a consistent picture of declining emissions, since first measured in 1969. This decline coincides with enhanced internal capture or recycling procedures and increasing use of physical and biological treatments. At the same time, and partly in response to legislative drivers, there has been an increase in the use of chronic (long-term) toxicity tests and alternative methods for assessing the quality of effluent discharges. The Whole Effluent Assessment (WEA) approach has also driven the increased conduct of studies addressing the fate of effluent constituents. Such studies have included the use of biodegradation and solid-phase micro-extraction-biomimetic extraction (SPME-BE) methods to address potentially bioaccumulative substances (PBS). In this way, it is then possible to address the persistence and toxicity of these PBS constituents of an effluent. The data collected in various case studies highlights the advantages and pitfalls of using biologically-based methods to assess the potential for refinery effluents to cause environmental impacts. Integr Environ Assess Manag 2015;X:000–000. ©2015 SETAC
Key Points
The use of biologically based effects methods to assess the quality of effluents and receiving waters is increasing in the EU primarily due to the focus of the Water Framework Directive on improving biological quality of receiving waters. Selection of test species, test design, and confounding issues in whole effluent toxicity tests can complicate data interpretation and need to be chosen carefully. Where effluent toxicity is observed in the EU, application of methods described in this paper indicates toxicity is not persistent or refractory. SPME coupled with effluent biodegradation studies provides a promising screening tool to assess the potential for bioaccumulation, additive toxicity, and even persistence of hydrocarbons in refinery effluents.
Computational Approaches to Understanding the Self‐assembly of Peptide‐based Nanostructures
Authors: Tuttle Tell 
Published: March 2015

AbstractThe interest in the self‐assembly of peptide‐based systems has grown significantly over the past 10–15 years, as more and more applications are shown to benefit from the useful properties of the amino acid based monomers. With the desire to apply the principals of self‐assembly to systems within new application areas, there has been an increasing emphasis in understanding the governing forces involved in the self‐assembly process, and using this understanding to predict the behaviour of, and design, new materials. To this end, computational approaches have played an increasingly important role over the past decade in helping to decode how small changes in the primary structure can lead to significantly different nanostructures with new function. In this review, a brief survey of the different computational approaches employed in this quest for understanding are provided, along with representative examples of the types of questions that can be answered with each of the different approaches.
A novel in chemico method to detect skin sensitizers in highly diluted reaction conditions
Authors: Yamamoto Yusuke , Tahara Haruna , Usami Ryota , Kasahara Toshihiko , Jimbo Yoshihiro , Hioki Takanori , Fujita Masaharu 
Published: March 2015

AbstractThe direct peptide reactivity assay (DPRA) is a simple and versatile alternative method for the evaluation of skin sensitization that involves the reaction of test chemicals with two peptides. However, this method requires concentrated solutions of test chemicals, and hydrophobic substances may not dissolve at the concentrations required. Furthermore, hydrophobic test chemicals may precipitate when added to the reaction solution. We previously established a high‐sensitivity method, the amino acid derivative reactivity assay (ADRA). This method uses novel cysteine (NAC) and novel lysine derivatives (NAL), which were synthesized by introducing a naphthalene ring to the amine group of cysteine and lysine residues. In this study, we modified the ADRA method by reducing the concentration of the test chemicals 100‐fold. We investigated the accuracy of skin sensitization predictions made using the modified method, which was designated the ADRA‐dilutional method (ADRA‐DM). The predictive accuracy of the ADRA‐DM for skin sensitization was 90% for 82 test chemicals which were also evaluated via the ADRA, and the predictive accuracy in the ADRA‐DM was higher than that in the ADRA and DPRA. Furthermore, no precipitation of test compounds was observed at the initiation of the ADRA‐DM reaction. These results show that the ADRA‐DM allowed the use of test chemicals at concentrations two orders of magnitude lower than that possible with the ADRA. In addition, ADRA‐DM does not have the restrictions on test compound solubility that were a major problem with the DPRA. Therefore, the ADRA‐DM is a versatile and useful method. Copyright © 2015 John Wiley & Sons, Ltd.
Investigation into the safety of perineural application of 1,4‐butanediol diglycidyl ether‐crosslinked hyaluronan in a rat model
Authors: Lan Sheng‐Min , Jou I‐Ming , Wu Po‐Ting , Wu Cheng‐Yi , Chen Sung‐Ching 
Published: March 2015

AbstractHyaluronan (HA) is well known for its biocompatibility and has widespread clinical use. To change its mechanical and physiologic properties to adapt to specific clinical scenarios, HA is crosslinked with chemically reactive linker molecules, most of which are toxic chemical reagents. Adverse events related to clinical use of crosslinked HA have been documented. Although approved by the FDA as dermal filler, the safety of perineural application of 1,4‐butanediol diglycidyl ether (BDDE)‐crosslinked HA has not been assessed critically. Concern exists owing to the vulnerability of neural tissues, because of their elongated morphology, high ratio of membrane surface area to cell volume, and complicated electrophysiologic properties. In this study, we systematically investigated the toxicity profile of BDDE‐crosslinked HA, usingin vitroandin vivoexperiments in a rat model. Thein vivoexperiments included the evaluation of aspects of histopathology, electrophysiology, and neurobehavior. There were no significant changes in the treatment group compared with the control group in all aspects of the experiments, except for the increased epineurial vascular formation in the 0.5% crosslinked HA‐treated group during 2 weeks of observation. Further studies involving perineural application of BDDE‐crosslinked HA can be done based on our findings, which ruled out the safety concern of cytotoxicity and adverse changes in electrophysiology and neurobehavior. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 718–726, 2015.
Synthèse de tertiobutylcétones α‐azotées de type
Authors: Montignoul Claude , Richard Marie‐Josée , Vigne Christian , Giral Louis 
Published: March 2015

AbstractLa synthèse des cétones 2, 3 et 4 a été réalisée en alkylant le dérivé azoté correspondant par des cétones α‐bromées. Celles‐ci furent synthétisées par bromation de cétones intermédiaires, elles‐měmes préparées par alkylation de la pinacolone ou de la benzyltertiobutylcétone par certains dérivés halogénés. On a observé que la bromation des cétones dépendait de la nature de ces dérivés.
Effects of different maturity stages and growing locations on changes in chemical, biochemical and aroma volatile composition of ‘Wonderful’ pomegranate juice
Authors: Mphahlele Rebogile R , Caleb Oluwafemi J , Fawole Olaniyi A , Opara Umezuruike Linus 
Published: March 2015

AbstractBACKGROUNDThis study investigated the changes in chemical attributes of pomegranate fruit such as total soluble solids (TSS), titratable acidity (TA), TSS/TA ratio, pH, individual compounds (organic acids and sugars) and volatile composition as affected by fruit maturity status and growing location (Kakamas, Koedoeshoek and Worcester in South Africa). Headspace solid phase microextraction coupled with gas chromatography/mass spectrometry was used for volatile analysis.RESULTSA significant increase in TSS from 14.7 ± 0.6 to 17.5 ± 0.6 °Brix was observed with advancement in fruit maturity, while TA decreased from 2.1 ± 0.7 to 1.1 ± 0.3 g citric acid per 100 mL across all agro‐climatic locations investigated. Fruit TSS/TA ratio and pH increased from 7.8 ± 2.6 to 16.6 ± 2.8 and from 3.3 ± 0.1 to 3.6 ± 0.2 respectively during fruit maturation across all agro‐climatic locations. Fructose and glucose concentrations increased continually with fruit maturity from 69.4 ± 4.9 to 91.1 ± 4.9 g kg−1 and from 57.1 ± 4.7 to 84.3 ± 5.2 g kg−1 respectively. A total of 13 volatile compounds were detected and identified, belonging to five chemical classes. The most abundant volatile in unripe and mid‐ripe fruit was 1‐hexanol, while 3‐hexen‐1‐ol was highest at commercial maturity.CONCLUSIONKnowledge on the impact of fruit maturity and agro‐climatic locations (with different altitudes) on biochemical and aroma volatile attributes of pomegranate fruit provides a useful guide for selecting farm location towards improving fruit quality and the maturity stage best for juice processing. © 2015 Society of Chemical Industry
Managing habitat to slow or reverse population declines of the Columbia spotted frog in the Northern Great Basin
Authors: Pilliod David S. , Scherer Rick D. 
Published: March 2015

ABSTRACTEvaluating the effectiveness of habitat management actions is critical to adaptive management strategies for conservation of imperiled species. We quantified the response of a Great Basin population of the Columbia spotted frog (Rana luteiventris) to multiple habitat improvement actions aimed to reduce threats and reverse population declines. We used mark‐recapture data for 1,394 adult frogs that had been marked by state, federal, and university biologists in 9 ponds representing a single population over a 16‐year period from 1997 to 2012. With the use of demographic models, we assessed population‐level effects of 1) a grazing exclosure constructed around 6 stock ponds that had been used to water livestock for decades before being fully fenced in 2003, and 2) the construction of 3 new stock ponds in 2003 to provide alternative water sources for livestock and, secondarily, to provide additional frog habitat. These management actions were implemented in response to a decline of more than 80% in population size from 1997 to 2002. We found evidence that excluding cattle from ponds and surrounding riparian habitats resulted in higher levels of frog production (more egg masses), higher adult frog recruitment and survival, and higher population growth rate. We also found that frogs colonized the newly constructed stock ponds within 3 years and frogs began breeding in 2 of them after 5 years. The positive effects of the cattle exclosure and additional production from the new ponds, although notable, did not result in full recovery of the population even 9 years later. This slow recovery may be partly explained by the effects of weather on recruitment rates, particularly the negative effects of harsher winters with late springs and higher fall temperatures. Although our findings point to potential successes of habitat management aimed at slowing or reversing rapidly declining frog populations, our study also suggests that recovering from severe population declines can take many years because of demographic and environmental processes. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.