bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co‐infection by Botryosphaeriaceae and Ilyonectria spp. fungi during propagation causes decline of young grafted grapevines
Authors: Whitelaw‐Weckert M. A. , Rahman L. , Appleby L. M. , Hall A. , Clark A. C. , Waite H. , Hardie W. J. 
Published: April 2013

Decline of newly planted, grafted grapevines is a serious viticultural problem worldwide. In the Riverina (New South Wales, Australia), characteristic symptoms include low fruit yields, very short shoots and severely stunted roots with black, sunken, necrotic lesions. To determine the cause, roots and wood tissue from affected plants in 20 vineyards (Vitis vinifera cv. Chardonnay grafted to V. champini cv. Ramsey rootstock) were assayed for microbial pathogens. Ilyonectria spp. (I. macrodidyma or I. liriodendra, producers of phytotoxin brefeldin A, BFA, and cause of black foot disease of grapevines) and Botryosphaeriaceae spp. (predominantly Diplodia seriata) were isolated from rootstocks of 100 and 95% of the plants, respectively. Togninia minima and Phaeomoniella chlamydospora (cause of grapevine Petri disease) were isolated from 13 and 7% of affected plants, respectively. All Ramsey rootstock stems of grafted plants sampled from a supplier nursery were infected with Ilyonectria spp. and D. seriata. Diplodia seriata, but not Ilyonectria spp., was also isolated from 25% of canes sampled from the rootstock source block. Root inoculation of potted, disease‐free Chardonnay plants with Ilyonectria isolates from diseased vineyards caused typical disease symptoms, while co‐inoculation with Botryosphaeriaceae spp. increased disease severity. This is the first study to show that a major cause of young grapevine decline can be sequential infection by Botryosphaeriaceae from rootstock cuttings and Ilyonectria spp. from nursery soil. Although the Petri disease fungi were less common in young declining grafted grapevines in the Riverina, they are likely to contribute to the decline of surviving plants as they mature.
The “red‐alert” effect in visual search: Evidence from human electrophysiology
Authors: Fortier‐Gauthier Ulysse , Dell'Acqua Roberto , Jolicœur Pierre 
Published: April 2013

AbstractParticipants had to determine the orientation of a segment inside a target color circle among other gray distractor circles. The target circle was either red or green and was accompanied in the display by a distractor in the other color. To dissociate event‐related potentials of target and distractor processing, one of them was on the vertical meridian and the other in a lateral position. In Experiment 1, the target color was indicated on a per‐trial basis and, in Experiment 2, on a per‐block basis. The results revealed the N2pc elicited by red targets had an earlier latency relative to the N2pc elicited by green targets. Contralateral responses of positive polarity linked to distractor inhibition were found only with red lateral distractors. The results suggest that the choice of colors to distinguish targets from distractors may play a role in visual search performance and in the functional characterization of event‐related lateralizations.
‘I am afraid to make the damage worse’ – fear of engaging in physical activity among patients with neck or back pain – a gender perspective
Authors: Stenberg Gunilla , Fjellman‐Wiklund Anncristine , Ahlgren Christina 
Published: April 2013

RationaleNeck and back pain are major public health problems in Western societies and cause considerable disability and health service use. Swedish women report more severe neck and back pain compared with Swedish men. Most studies on the aetiology of gender differences in pain deal with biological mechanisms, and less with the role of psychological and sociocultural factors. ‘Pain beliefs’ is a sociocultural factor and can be expressed in different ways among women and men. It is important to know what pain beliefs are held by neck and back pain patients, especially when medical guidelines recommend that back pain patients stay physically active.AimExploring pain beliefs in relation to physical activity among neck and back pain patients consulting primary health care.MethodTwelve patients (seven women, five men) consulting primary health care for an initial episode of neck or back pain were interviewed before their first appointment with a physiotherapist or general practitioner and 3 months later. The interviews covered patient experiences of neck or back pain, consequences, strategies and treatment experiences. The interviews were analysed with qualitative content analysis from a gender perspective.ResultOne theme ‘Fear of hurting the fragile body’ was expressed by all neck or back pain patients. Five categories were identified ‘The mechanical body’, ‘Messages about activity’, ‘Earlier experiences of pain and activity’, ‘To be a good citizen’ and ‘Support to be active’ supported or undermined beliefs about pain and physical activity. Gender expressions occurred in the categories ‘Messages about activity’, ‘To be a good citizen’ and ‘Support to be active’.ConclusionsNeck or back pain patients in the study saw the body as fragile and were afraid of hurting it. Notions of gender had an impact on the given advice about activity and on how patients perceived the message about staying active.
Loneliness, self‐esteem, and life satisfaction as predictors of Internet addiction: A cross‐sectional study among Turkish university students
Authors: Bozoglan Bahadir , Demirer Veysel , Sahin Ismail 
Published: April 2013

This study investigated the relationship among loneliness, self‐esteem, life satisfaction, and Internet addiction. Participants were 384 university students (114 males, 270 females) from 18 to 24 years old from the faculty of education in Turkey. The Internet Addiction, UCLA Loneliness, Self‐esteem, and Life Satisfaction scales were distributed to about 1000 university students, and 38.4% completed the survey (see Appendix A and B). It was found that loneliness, self‐esteem, and life satisfaction explained 38% of the total variance in Internet addiction. Loneliness was the most important variable associated with Internet addiction and its subscales. Loneliness and self‐esteem together explained time‐management problems and interpersonal and health problems while loneliness, self‐esteem, and life satisfaction together explained only the interpersonal and health problems subscales.
Fatal herpes simplex virus type 2 hepatitis in a heart transplant recipient: a case report and review of the literature
Authors: Pietrucha‐Dilanchian P. , Tanawuttiwat T. , Abbo L. , Regatieri A. , Chaparro S. , Ruiz P. , Morris M.I. 
Published: April 2013

AbstractHerpes simplex virus (HSV) hepatitis is often unrecognized clinically with most untreated cases diagnosed postmortem. HSV hepatitis has been reported in solid organ transplant (SOT) recipients, mostly in kidney and liver transplants, and rarely in heart transplant recipients. We describe a fatal case of community‐acquired HSV‐2 hepatitis in a 24‐year‐old heart transplant recipient occurring 3 years after transplant. We also review the literature summarizing HSV hepatitis and the potential role of quantitative HSV polymerase chain reaction monitoring in the SOT population.
Qualitative analysis of patients’ feedback from a PROMs survey of cancer patients in England
Authors: Corner Jessica , Wagland Richard , Glaser Adam , Richards Sir Mike 
Published: April 2013

ObjectiveThis study examined how free-text comments from cancer survivors could complement formal patient-reported outcome measures (PROMs), as part of the England PROMs survey programme for cancer.DesignA qualitative content analysis was conducted of responses to a single open-ended free-text question placed at the end of the cross-sectional population-based postal questionnaire.SettingIndividuals were identified through three UK Cancer Registries and questionnaires were posted to their home addresses.ParticipantsA random sample of individuals (n=4992) diagnosed with breast, colorectal, non-Hodgkins lymphoma or prostate cancer at 1, 2, 3 and 5 years earlier.Results3300 participants completed the survey (68% response rate). Of these 1056 (32%) completed the free-text comments box, indicating a high level of commitment to provide written feedback on patient experience. Almost a fifth (19%) related experiences of excellent care during the treatment phase, with only 8% reporting negative experiences. This contrasted with experiences of care after primary cancer treatment where the majority were negative. Factors impacting negatively upon patient-reported outcomes included the emotional impact of cancer; poor experiences of treatment and care; comorbidities, treatment side effects, social difficulties and inadequate preparation for a wide range of sometimes long-lasting on-going physical and psychological problems. Mediating factors assisting recovery incorporated both professional-led factors, such as quality of preparation for anticipated problems and aftercare services, and participant-led factors, such as learning from other cancer survivors and self-learning through trial and error. The support of friends and family was also a factor in participants' outcomes.ConclusionsThis analysis of free-text comments complements quantitative analysis of PROMs measure's by illuminating relationships between factors that impact on quality of life (QoL) and indicate why cancer patients may experience significantly worse QoL than the general population. The data suggest more systematic preparation and aftercare for individuals to self-manage post-treatment problems might improve QoL outcomes among cancer survivors.
Assessing the efficacy of the electronic patient record system EDeR: implementation study—study protocol
Authors: Job Oliver , Bachmann Lucas M , Schmid Martin K , Thiel Michael A , Ivic Sandra 
Published: April 2013

IntroductionDespite many innovations in information technology, many clinics still rely on paper-based medical records. Critics, however, claim that they are hard to read, because of illegible handwriting, and uncomfortable to use. Moreover, a chronological overview is not always easily possible, content can be destroyed or get lost. There is an overall opinion that electronic medical records (EMRs) should solve these problems and improve physicians’ efficiency, patients’ safety and reduce the overall costs in practice. However, to date, the evidence supporting this view is sparse.Methods and analysisIn this protocol, we describe a study exploring differences in speed and accuracy when searching clinical information using the paper-based patient record or the Elektronische DateneRfassung (EDeR). Designed as a randomised vignette study, we hypothesise that the EDeR increases efficiency, that is, reduces time on reading the patient history and looking for relevant examination results, helps finding mistakes and missing information quicker and more reliably. In exploratory analyses, we aim at exploring factors associated with a higher performance.Ethics and disseminationThe ethics committee of the Canton Lucerne, Switzerland, approved this study. We presume that the implementation of the EMR software EDeR will have a positive impact on the efficiency of the doctors, which will result in an increase of consultations per day. We believe that the results of our study will provide a valid basis to quantify the added value of an EMR system in an ophthalmological environment.
The new variant ofChlamydia trachomatiswas present as early as 2003 in Örebro County, Sweden, but remained undetected until 2006
Authors: Jurstrand Margaretha , Fredlund Hans , Unemo Magnus 
Published: April 2013

ObjectivesIn 2006, a new variant ofChlamydia trachomatis(nvCT) was reported in Sweden. Because of a cryptic plasmid deletion, the nvCT was undetectable in several of the genetic diagnostic systems used worldwide at the time. This study aimed to evaluate whether the nvCT was present in specimens obtained from patients attending the outpatient sexually transmitted infection (STI) clinic at Örebro University Hospital, Örebro, Sweden already in 2002–2003.MethodsIn 2012, archival (−20°C freezer) urogenital specimens (2002 (n=1083) and in 2003 (n=1143)) obtained from men (2002 (n=398) and 2003 (n=486)) and women (2002 (n=301) and 2003 (n=408)) were analysed with Cobas TaqMan CT test V.2.0. AllC trachomatispositive specimens were subsequently examined using a duplex PCR assay that simultaneously detects the deletion on the nvCT cryptic plasmid and theompAgene ofC trachomatisgenotype E.ResultsIn total, 68 patients (9.7%) in 2002 and 61 (6.8%) in 2003 wereC trachomatispositive. The duplex PCR assay identified 26C trachomatisgenotype E positive patients in 2002 (38%) and 25 in 2003 (41%). No nvCT was found in 2002, but one specimen obtained from a 23-year-old man in June 2003 was positive for the nvCT.ConclusionsThe nvCT was present as early as 2003 in Örebro County, Sweden, which concurs with previously reported statistical estimations of its emergence. Accordingly, the nvCT spread undetected for at least 3 years, explaining the high proportion (38%) in Örebro County when it was first detected in late 2006.
Salt pillows and localization of early structures: case study in the Ucayali Basin (Peru)
Authors: Moretti I. , Callot J. P. , Principaud M. , Pillot D. 
Published: April 2013

Abstract
In many compressive zones, there is a risk of undercharged hydrocarbon prospects as a result of timing, that is, the growth of the structure is younger than the main fluid migration phase. The North Ucayali Basin represents a setting of this type, where locating the earliest structures is crucial for well placement. In the North Ucayali Basin, the variable amount of erosion at the top of the structures shows that they are not uniformly recent. Although the growth of early structures may be explained by the reactivation of inherited features during shortening, evaporitic pillows may represent an alternative factor controlling the localization of deformation in the studied area. Indeed, subsurface data reveal the presence of flat salt domes that have an influence on thrust localization. The existence and tips of such efficient gliding surfaces concentrate the strain and, as a result, localize the early zones of deformation. Analogue models designed to study these phenomena highlight the crucial role of salt pillows as potential weak zones localizing deformation ahead of the propagating wedge. These models also emphasize the need to constrain certain parameters that have been previously disregarded, including the thickness of the brittle layers between the main décollement level (if present) and the pillows, as well as their burial depth.
Single Copies of Mutant KRAS and Mutant PIK3CA Cooperate in Immortalized Human Epithelial Cells to Induce Tumor Formation
Authors: Wang Grace M. , Wong Hong Yuen , Konishi Hiroyuki , Blair Brian G. , Abukhdeir Abde M. , Gustin John P. , Rosen D. Marc , Denmeade Samuel Ray , Rasheed Zeshaan , Matsui William , Garay Joseph P. , Mohseni Morassa , Higgins Michaela J. , Cidado Justin , Jelovac Danijela , Croessmann Sarah , Cochran Rory L. , Karnan Sivasundaram , Konishi Yuko , Ota Akinobu , Hosokawa Yoshitaka , Argani Pedram , Lauring Josh , Park Ben Ho 
Published: April 2013

Abstract
The selective pressures leading to cancers with mutations in both KRAS and PIK3CA are unclear. Here, we show that somatic cell knockin of both KRAS G12V and oncogenic PIK3CA mutations in human breast epithelial cells results in cooperative activation of the phosphoinositide 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways in vitro, and leads to tumor formation in immunocompromised mice. Xenografts from double-knockin cells retain single copies of mutant KRAS and PIK3CA, suggesting that tumor formation does not require increased copy number of either oncogene, and these results were also observed in human colorectal cancer specimens. Mechanistically, the cooperativity between mutant KRAS and PIK3CA is mediated in part by Ras/p110α binding, as inactivating point mutations within the Ras-binding domain of PIK3CA significantly abates pathway signaling. In addition, Pdk1 activation of the downstream effector p90RSK is also increased by the combined presence of mutant KRAS and PIK3CA. These results provide new insights into mutant KRAS function and its role in carcinogenesis. Cancer Res; 73(11); 3248–61. ©2013 AACR.
MTA1 Promotes STAT3 Transcription and Pulmonary Metastasis in Breast Cancer
Authors: Pakala Suresh B. , Rayala Suresh K. , Wang Rui-An , Ohshiro Kazufumi , Mudvari Prakriti , Reddy Sirigiri Divijendra Natha , Zheng Yi , Pires Ricardo , Casimiro Sandra , Pillai M. Radhakrishna , Costa Luis , Kumar Rakesh 
Published: April 2013

Abstract
Overexpression of the prometastatic chromatin modifier protein metastasis tumor antigen 1 (MTA1) in human cancer contributes to tumor aggressiveness, but the role of endogenous MTA1 in cancer has not been explored. Here, we report the effects of selective genetic depletion of MTA1 in a physiologically relevant spontaneous mouse model of breast cancer pulmonary metastasis. We found that MTA1 acts as a mandatory modifier of breast-to-lung metastasis without effects on primary tumor formation. The underlying mechanism involved MTA1-dependent stimulation of STAT3 transcription through action on the MTA1/STAT3/Pol II coactivator complex, and, in turn, on the expression and functions of STAT3 target genes including Twist1. Accordingly, we documented a positive correlation between levels of MTA1 and STAT3 in publicly available breast cancer data sets. Together, our findings reveal an essential modifying role of the physiologic level of MTA1 in supporting pulmonary metastasis of breast cancer. Cancer Res; 73(12); 3761–70. ©2013 AACR.
Diabetes Mellitus and Prostate Cancer Risk; A Nationwide Case–Control Study within PCBaSe Sweden
Authors: Fall Katja , Garmo Hans , Gudbjörnsdottir Soffia , Stattin Pär , Zethelius Björn 
Published: April 2013

Abstract
Background: Diabetes mellitus (DM) increases the risk for cancer at almost all sites, but data on the association with prostate cancer are inconsistent.
Methods: We assessed the risk of a prostate cancer diagnosis among men with type 2 (T2)DM in a nationwide population-based case-control study including 44,352 men with prostate cancer identified through the Prostate Cancer data Base Sweden (PCBaSe) between 2002 and 2006 and 221,495 age-matched men from the general population.
Results: Overall, the risk of prostate cancer among men with T2DM was lower than among men without T2DM [OR, 0.80; 95% confidence interval (CI), 0.76–0.85]. The risk decreased with longer disease duration and was observed across all tumor risk categories, although most clearly among men with low risk tumors (OR, 0.71; 95% CI, 0.64–0.80). The risk for prostate cancer was reduced among diabetic men on dietary treatment only (OR, 0.89; 95% CI, 0.80–0.99) but more markedly among men on oral hypoglycemic agents (OR, 0.80; 95% CI, 0.74–0.87) and insulin (OR, 0.72; 95% CI, 0.69–0.81). Obese diabetic men (BMI &gt; 30 kg/m2) showed a reduced risk (OR, 0.72; 95% CI, 0.65–0.80) compared with men without diabetes. There was a trend of decreasing risk with increasing levels of HbA1c (P &lt; 0.05).
Conclusions: This nationwide study confirmed a reduced risk of being diagnosed with prostate cancer among men with T2DM, especially for low-risk tumors. An altered hormonal milieu is a plausible explanation, although the possibility of decreased prostate cancer detection among diabetic men cannot be ruled out.
Impact: This is the largest study to examine the association between T2DM and prostate cancer accounting for tumor risk group and diabetes treatment. Cancer Epidemiol Biomarkers Prev; 22(6); 1102–9. ©2013 AACR.
Smokeless and Dual Tobacco Use Among Males Surviving Childhood Cancer: A Report From the Childhood Cancer Survivor Study
Authors: Klosky James L. , Hum Ashley M. , Zhang Nan , Ali Khatidja S. , Srivastava D. Kumar , Klesges Robert C. , Emmons Karen M. , Ness Kirsten K. , Stovall Marilyn , Robison Leslie L. , Hudson Melissa M. 
Published: April 2013

Abstract
Cancer survivors experience treatment-related complications that can be exacerbated by tobacco use. This study reports the prevalence of smokeless and dual tobacco use, compares these rates to the U.S. population, and examines tobacco risk factors among males surviving childhood cancer. Data from the Childhood Cancer Survivor Study (CCSS) 2007 survey were used (N = 3378). Standardized incidence ratios (SIR) were obtained by comparing CCSS data with the National Survey on Drug Use and Health. Logistic regression was used to evaluate associations between risk factors and tobacco use. Among male survivors, 8.3% and 2.3% were current smokeless tobacco and dual tobacco users, respectively. Survivors were less likely than population males to report smokeless tobacco [SIR = 0.64; 95% confidence interval (CI), 0.57–0.72) or dual tobacco (SIR = 0.37; CI, 0.29–0.46) use; however, non-White survivors aged 35 to 49 years were more likely to use smokeless tobacco (SIR = 2.32; CI, 1.27–3.90). Smokeless tobacco use was associated (P &lt; 0.05) with younger age at diagnosis, lower education, being married or divorced/separated, and not living in the Northeastern United State, whereas history of cardiovascular- and/or pulmonary-toxic treatment was protective. Dual tobacco use was associated with younger age at diagnosis, lower education, divorce/separation, and high psychologic distress. Having active heart or circulatory conditions was protective. Although smokeless tobacco/dual tobacco use is generally low among childhood cancer survivors, these findings suggest that tobacco use screening should be expanded to include smokeless tobacco use, and that smokeless tobacco-specific education and cessation interventions should be provided to users. Screening and intervening for smokeless tobacco/dual tobacco use in childhood cancer survivors will reduce tobacco-related morbidity and mortality. Cancer Epidemiol Biomarkers Prev; 22(6); 1025–9. ©2013 AACR.
A Proposal Regarding Reporting of In Vitro Testing Results
Authors: Smith Malcolm A. , Houghton Peter 
Published: April 2013

Abstract
The high rate of negative clinical trials and failed drug development programs calls into question the use of preclinical testing as currently practiced. An important issue for the in vitro testing of agents that have advanced into the clinic is the use of clinically irrelevant concentrations in reports making claims for anticancer activity, as illustrated by publications for sorafenib, vorinostat, and metformin. For sorafenib, high protein binding leads to a dichotomy between concentrations active in the 10% serum conditions commonly used for in vitro testing and concentrations active in plasma. Failure to recognize this distinction leads to inappropriate claims of activity for sorafenib based on the micromolar concentrations commonly used for in vitro testing in low serum conditions. For vorinostat and metformin, results using in vitro concentrations higher than those achievable in patients are reported despite the availability of publications describing human pharmacokinetic data for each agent. We encourage journal editors and reviewers to pay greater attention to clinically relevant concentrations when considering reports that include in vitro testing of agents for which human pharmacokinetic data are available. Steps taken to more carefully scrutinize activity claims based on in vitro results can help direct researchers away from clinically irrelevant lines of research and toward lines of research that are more likely to lead to positive clinical trials and to improved treatments for patients with cancer. Clin Cancer Res; 19(11); 2828–33. ©2013 AACR.