bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multistimuli‐Responsive Luminescence of Naphthalazine Based on Aggregation‐Induced Emission
Authors: Yao Xiang , Ru Jia‐Xi , Xu Cong , Liu Ya‐Ming , Dou Wei , Tang Xiao‐Liang , Zhang Guo‐Lin , Liu Wei‐Sheng 
Published: April 2015

AbstractStimuli‐responsive luminescent materials, which are dependent on changes in physical molecular packing modes, have attracted more and more interest over the past ten years. In this study, 2,2‐dihydroxy‐1,1‐naphthalazine was synthesized and shown to exhibit different fluorescence emission in solution and solid states with characteristic aggregation‐induced emission (AIE) properties. A remarkable change in the fluorescence of 2,2‐dihydroxy‐1,1‐naphthalazine occurred upon mechanical grinding, heating, or exposure to solvents. According to the characterization by solid‐state fluorescence spectroscopy, X‐ray crystallography, differential scanning calorimetry, and X‐ray powder diffraction, the fluorescence change could be attributed to transitions between two structurally different polymorphs. These significant properties could also give 2,2‐dihydroxy‐1,1‐naphthalazine more potential applications as a multifunctional material.
New Imaging Techniques on the RISE
Authors: Hollricher Olaf , Schmidt Ute , Breuninger Sonja 
Published: April 2015

AbstractThe implementation of correlative microscopy techniques for materials and surface analysis has grown steadily in recent years. This trend is being driven by the increasing demands placed on analytical methods for detailed and comprehensive sample investigations. WITec's Raman Imaging and Scanning Electron (RISE) microscope is the latest advance in this field.
Count on Your Images
Authors: Briggs Stephan , McCall Brian 
Published: April 2015

AbstractThe history of microscopy is the story of the quest to obtain ever more reliable information about a world that is invisible to the naked eye. The development of stains introduced the ability to distinguish distinct cells, then distinct cellular organelles; the design of optically corrected multi‐element objectives enhanced the fidelity of microscopic imaging; the introduction of phase contrast microscopy enhanced the ability to isolate different features in a sample. Fluorescence microscopy is one approach — almost inarguably the most promising and the most valuable — to extending the quality of information available from an image.
Rapid Microstructure Analysis of Polar Ice Cores
Authors: Krischke Anja , Oechsner Ulrich , Kipfstuhl Sepp 
Published: April 2015

AbstractWith climate change and its implications for society and the Earth being a frequent topic in both politics and science, measureable data on the influence of mankind on current and past climate has become essential information for making predictions and decisions about future climate. The polar ice sheet provides information about temperature, precipitation as well as gas and aerosol concentration as a unique depiction of climate throughout hundreds of thousands of years. The information obtained from ice cores enables future climatic events as well as general material properties of ice to be better understood. The longest ice core drilled in Antarctica has a length of 3270 m and contains climate information dating back more than 800,000 years. The rapid analysis (minimum scan time 3 s) provided by the large area scan macroscope (LASM; Fig. 1) with a resolution of 5 μm has proven to be an essential tool for analyzing the microstructures of ice cores, both in the field and in the laboratory. A stratigraphic image that supports dating the ice cores can be obtained using Intermediate Layer Core Scanner.
Determination of triglycidyl isocyanurate from air samples by ultra‐performance liquid chromatography coupled with coordination ion spray mass spectrometry
Authors: Gagné Sébastien , Carrier Marielle , Aubin Simon 
Published: April 2015

RationaleUltra‐performance liquid chromatography (UPLC) coupled with coordination ion spray tandem mass spectrometry was used for the analysis of air samples containing triglycidyl isocyanurate. The method is not affected by any chromatographic interference and the filter extract is compatible with the UPLC system, as opposed to approaches using high‐performance liquid chromatography coupled with ultraviolet detection and gas chromatography coupled with mass spectrometry.MethodsAccu‐cap™ filters that had sampled triglycidyl isocyanurate were extracted using a mixture of acetonitrile/acetone (95/5) diluted with 3 volumes of water and were then analyzed. The mass spectrometry method uses sodium as the alkali adduct complexing with the triglycidyl isocyanurate in positive mode, and this complex is then analyzed using the survivor mode where the same ion is monitored in the first and third quadrupoles of a triple quadrupole mass spectrometer.ResultsThe method has a limit of detection and limit of quantitation of 50 and 170 ng/filter, respectively. The dynamic range was between 480 and 24,000 ng/sample, which is equivalent to 2 µg/m3 and 100 µg/m3 based on a sampling volume of 240 L. The intra‐ and inter‐day precisions were both <4% and the overall accuracy was 97 ± 3%. The method was tested with personal breathing zone random samples collected from workers using triglycidyl isocyanurate in their tasks, and all the random samples were easily quantified.ConclusionsA new method by UPLC coupled with coordination ion spray tandem mass spectrometry using sodium as the alkali adduct is now available for industrial hygienists who want to evaluate exposures of workers to triglycidyl isocyanurate in workplaces. Copyright © 2015 John Wiley & Sons, Ltd.
Quantifying non‐covalent binding affinity using mass spectrometry: A systematic study on complexes of cyclodextrins with alkali metal cations
Authors: Wei Wanghui , Chu Yanqiu , Wang Rizhi , He Xiaodan , Ding Chuanfan 
Published: April 2015

RationaleTo date, the quantification of binding affinities for non‐covalent complexes between cyclodextrin (CD) and alkali cations including Li+, Na+, K+, Rb+, and Cs+ has not been investigated in detail by electrospray ionization mass spectrometry (ESI‐MS) due to the unknown ionization efficiencies of the different species. In this study, the binding constants of CD‐Cs+ complexes were determined by an improved mass spectrometric titration methodology, which was based only on the peak intensities of equilibrium CD. Hence, the discrepancy of ionization efficiencies of CD, alkaline cation and their complex would not affect the measurement. Then the obtained lgKa values were provided as references for competitive ESI‐MS. The binding constants for complexes of α‐, β‐ or γ‐CD with Li+, Na+, K+ and Rb+ could be derived directly and quickly.MethodsThe lgKa values between α‐, β‐ or γ‐CD and Cs+ data were processed by curve fitting. These lgKa values were provided as references for competitive ESI‐MS. In addition, linear fit equations for complexes of α‐, β‐ or γ‐CD with Cs+ were derived. Through the linear fit equations of competitive ESI‐MS, the binding constants for complexes of Li+, Na+, K+ and Rb+ with α‐, β‐ or γ‐CD were acquired.ResultsResults showed that the binding constant (lgKa) values for the complexes of Cs+ with α‐, β‐ and γ‐cyclodextrins were 3.94, 3.88 and 3.80, respectively, revealing that the binding strength decreased with the increase in diameter of cyclodextrins. The competitive ESI‐MS results showed a clear trend of decreasing affinity for complexes of cyclodextrins in the order of Li+, Na+, K+, Rb+.ConclusionsThe binding constants of non‐covalent cyclodextrin‐alkali cation complexes have been systematically studied by an improved mass spectrometric titration and competitive ESI‐MS. Also, the structural features of the complexes were discussed. Our results are valuable for better understanding of mechanisms driving inclusion chemistry under well‐defined conditions. Copyright © 2015 John Wiley & Sons, Ltd.
Determination of interglycosidic linkages in O‐glycosyl flavones by high‐performance liquid chromatography/photodiode‐array detection coupled to electrospray ionization ion trap mass spectrometry. Its application to Tetragonula carbonaria honey from Australia
Authors: Truchado Pilar , Vit Patricia , Heard Tim A. , Tomás‐Barberán Francisco A. , Ferreres Federico 
Published: April 2015

RationaleTetragonula carbonaria pot‐honeys are highly valued as a food source and for their biological activities in Australia, and there is a growing interest to know its composition. Phenolic metabolites, which could be related to their beneficial properties, have not been studied in depth yet.MethodsMass spectrometry (MS) coupled to liquid chromatography (LC) is an advanced technique for the study of complex flavonoids present in difficult food matrices that hampers their isolation and purification. This allows the tentative characterization of diglycosides/triglycosides establishing the position of the O‐glycosylation on the sugar moiety by the study of the MS data in T. carbonaria pot‐honeys from Australia.ResultsTheir spectra obtained by high‐performance liquid chromatography/photodiode‐array detection/electrospray ionization ion trap mass spectrometry (HPLC/DAD/ESI‐MSn) revealed for the first time 19 quercetin, kaempferol and isorhamnetin O‐glycosides. These compounds were clustered in flavonoid triglycosides, diglycosides and monoglycosides. The first cluster contained one flavonoid trihexoside, two −3‐O‐(2‐hexosyl, 6‐rhamnosyl)hexosides and their isomers and two −3‐O‐(2,6‐di‐rhamnosyl)hexosides. In the second cluster, eleven flavonoid diglycosides such as three −3‐O‐(2‐hexosyl)hexosides, four −3‐O‐(2‐rhamnosyl)hexosides and one −3‐O‐(6‐rhamnosyl)hexoside as well as two −3‐O‐(2‐pentosyl)hexosides and one tentative −3‐O‐(3‐pentosyl)hexoside were detected. In the monoglycoside group, only one flavonoid −3‐O‐hexoside was identified.ConclusionsThe occurrence of this large number of flavonoid glycosides could be due to the low glucosidase activity previously reported in stingless bee honey. Copyright © 2015 John Wiley & Sons, Ltd.
Identification and relative quantification of phosphopeptides by a combination of multi‐protease digestion and isobaric labeling
Authors: Linke Dennis , Koudelka Tomas , Becker Alexander , Tholey Andreas 
Published: April 2015

RationaleThe identification and the determination of the extent of protein phosphorylation are major prerequisites for the comparative analysis of this important posttranslational modification of proteins in different biological situations. High sequence coverages and the availability of straightforward quantification methods are necessary to achieve these goals.MethodsPhosphoproteins and non‐phosphorylated analogues separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) were digested using four different proteases (trypsin, chymotrypsin, elastase and GluC) and the digests were isobarically labeled using eight‐plex iTRAQ. The combined labeled digests were subsequently enriched using titanium dioxide and both the phosphorylated and non‐phosphorylated fractions were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). The influence of different data analysis algorithms (Percolator or False Discovery Rate) on the outcome of analysis was investigated.ResultsAlmost complete sequence coverage could be achieved upon application of a multi‐protease approach. The formation of peptides of different lengths and physicochemical properties allowed the identification and the mapping of all phosphorylation sites in the investigated model proteins. The introduction of isobaric labels allowed quantification of different peptides of the same phosphorylation site with more than one peptide, leading to significantly improved statistical confidence.ConclusionsA workflow for the straightforward comparative analysis of protein phosphorylation in samples of low complexity, e.g. isolated proteins, was developed. The workflow is transferable to other posttranslational modifications. Copyright © 2015 John Wiley & Sons, Ltd.
Selective reduction in complicated monochorionic twins: prediction of obstetric outcome and comparison of techniques
Authors: Yinon Y. , Ashwal E. , Weisz B. , Chayen B. , Schiff E. , Lipitz S. 
Published: April 2015

AbstractObjectivesTo compare the outcomes of radiofrequency ablation (RFA) and bipolar cord coagulation (BPC) methods for selective feticide in complicated monochorionic (MC) twin gestations and to determine risk factors for adverse pregnancy outcome.MethodsThis was a retrospective cohort study of all patients with complicated MC twin pregnancy who underwent selective reduction in a single tertiary care center between 2000 and 2014. We compared the two methods with respect to various demographic, obstetric and perinatal outcome variables.ResultsFifty‐three selective terminations in complicated MC twin gestations were performed, 36 by RFA and 17 by BPC. Each technique resulted in a similar overall survival rate of the remaining twin (88.9% vs 76.5%, P = 0.25). Likewise, the mean procedure‐to‐delivery interval, the rate of spontaneous preterm delivery < 37, < 34, < 32 and < 28 weeks and the rate of preterm prelabor rupture of the membranes did not differ between the groups. However, among patients in the BPC group, there was a trend towards earlier gestational age at delivery (31.3 vs 35.0 weeks, P = 0.09) compared with in the RFA group. There was selective reduction by RFA due to selective intrauterine growth restriction (sIUGR) in 19 cases, 17 (89.5%) of which survived perinatally, with delivery at a median gestational age of 37 weeks. There were no significant differences in perinatal outcome, irrespective of whether the procedure was performed pre‐ or post‐viability. All post‐viability reductions, most of which were by RFA, resulted in perinatal survival.ConclusionsPerinatal outcome of complicated MC twin gestations undergoing selective feticide seems to be similar regardless of whether termination is by BPC or RFA. Nevertheless, RFA appears to be particularly useful in MC twins complicated by sIUGR and in cases requiring selective reduction beyond the age of viability. Copyright © 2015 ISUOG. Published by John Wiley & Sons Ltd.
Efficient production and evaluation of lignocellulolytic enzymes using a constitutive protein expression system in Penicillium oxalicum
Authors: Hu Yibo , Xue Haizhao , Liu Guodong , Song Xin , Qu Yinbo 
Published: April 2015

Abstract
Native lignocellulolytic enzyme systems secreted by filamentous fungi can be further optimized by protein engineering or supplementation of exogenous enzyme components. We developed a protein production and evaluation system in cellulase-producing fungus Penicillium oxalicum. First, by deleting the major amylase gene amy15A, a strain Δ15A producing few extracellular proteins on starch was constructed. Then, three lignocellulolytic enzymes (BGL4, Xyn10B, and Cel12A) with originally low expression levels were successfully expressed with selected constitutive promoters in strain Δ15A. BGL4 and Cel12A overexpression resulted in increased specific filter paper activity (FPA), while the overexpression of Xyn10B improved volumetric FPA but not specific FPA. By switching the culture medium, this platform is convenient to produce originally low-expressed lignocellulolytic enzymes in relatively high purities on starch and to evaluate the effect of their supplementation on the performance of a complex cellulase system on cellulose.
Clinical, morphological, biochemical, imaging and outcome parameters in 21 individuals with mitochondrial maintenance defect related to FBXL4 mutations
Authors: Huemer Martina , Karall Daniela , Schossig Anna , Abdenur Jose E. , Al Jasmi Fatma , Biagosch Caroline , Distelmaier Felix , Freisinger Peter , Graham Brett H. , Haack Tobias B. , Hauser Natalie , Hertecant Jozef , Ebrahimi‐Fakhari Darius , Konstantopoulou Vassiliki , Leydiker Karen , Lourenco Charles M. , Scholl‐Bürgi Sabine , Wilichowski Ekkehard , Wolf Nicole I. , Wortmann Saskia B. , Taylor Robert W. , Mayr Johannes A. , Bonnen Penelope E. , Sperl Wolfgang , Prokisch Holger , McFarland Robert 
Published: April 2015

AbstractFBXL4 deficiency is a recently described disorder of mitochondrial maintenance associated with a loss of mitochondrial DNA in cells. To date, the genetic diagnosis of FBXL4 deficiency has been established in 28 individuals. This paper retrospectively reviews proxy‐reported clinical and biochemical findings and evaluates brain imaging, morphological and genetic data in 21 of those patients. Neonatal/early‐onset severe lactic acidosis, muscular hypotonia, feeding problems and failure to thrive is the characteristic pattern at first presentation. Facial dysmorphic features are present in 67 % of cases. Seven children died (mean age 37 months); 11 children were alive (mean age at follow‐up 46 months), three children were lost to follow‐up. All survivors developed severe psychomotor retardation. Brain imaging was non‐specific in neonates but a later‐onset, rapidly progressive brain atrophy was noted. Elevated blood lactate and metabolic acidosis were observed in all individuals; creatine kinase was elevated in 45 % of measurements. Diagnostic workup in patient tissues and cells revealed a severe combined respiratory chain defect with a general decrease of enzymes associated with mitochondrial energy metabolism and a relative depletion of mitochondrial DNA content. Mutations were detected throughout the FBXL4 gene albeit with no clear delineation of a genotype‐phenotype correlation. Treatment with “mitochondrial medications” did not prove effective. In conclusion, a clinical pattern of early‐onset encephalopathy, persistent lactic acidosis, profound muscular hypotonia and typical facial dysmorphism should prompt initiation of molecular genetic analysis of FBXL4. Establishment of the diagnosis permits genetic counselling, prevents patients undergoing unhelpful diagnostic procedures and allows for accurate prognosis.
Coved‐type ST‐elevation during ablation of ischemic ventricular tachycardia
Authors: Hori Yuichi , Nakahara Shiro , Tsukada Naofumi , Nakagawa Ayako , Hayashi Akiko , Komatsu Takaaki , Kobayashi Sayuki , Sakai Yoshihiko , Taguchi Isao 
Published: April 2015

AbstractA coved‐type electrocardiogram (ECG) change in Brugada syndrome is suggested to be the result of abnormally delayed depolarization over the right ventricular outflow tract; however, ischemia of the conus branch of the right coronary artery presents the same ECG change. A 63‐year‐old man with a history of myocardial infarction demonstrated a transient coved‐type ECG change during catheter ablation of ventricular tachycardia. The ECG change appeared during left ventricular mapping without any chest symptoms, and recovered spontaneously. A pilsicainide test was negative and a coved‐type ECG did not appear during the perioperative or follow‐up period.
PRODUCER PARTICIPATION IN BIOMASS MARKETS: FARM FACTORS, MARKET FACTORS, AND CORRELATED CHOICES
Authors: HOQUE MOHAMMAD MAINUL , ARTZ GEORGEANNE M. , JARBOE DARREN H. , MARTENS BOBBY J. 
Published: April 2015

AbstractThis study evaluates attitudes toward production of three major types of biomass—corncobs, corn stover, and dedicated energy grass—using responses to a mail survey of 2,250 Iowa farmers. We examine how factors influencing willingness to supply biomass vary across biomass types and analyze the presence of potential correlations across interest in growing various types of biomass. We find that correlation in choices across biomass types is strong and statistically significant. Farmers consider corn stover and corncobs as complementary, but dedicated energy grasses and corncobs or stover as substitutes. Factors affecting farmer choices also vary significantly by type of biomass.
Meanings and Dimensions of Organizational Justice in China: An Inductive Investigation
Authors: Guo Chun , Giacobbe-Miller Jane K. 
Published: April 2015

ABSTRACTTaking an inductive approach followed by validation techniques, we analyzed the meaning and dimensionality of the organizational justice construct in the People's Republic of China (China). By using qualitative and quantitative methods, we found that organizational justice involves a higher level of subjectivity in the Chinese context. Interpretation and organizational justice judgments depend on the specific situation and social relations involved. The findings revealed similarities with the Western conceptualization with one important exception, suggesting both cultural values and institutional environment shape justice judgments and managerial practices in China. The article concludes with a discussion of the findings and implications for management theory, research, and practice.
A high-cholesterol diet enriched with polyphenols from Oriental plums (Prunus salicina) improves cognitive function and lowers brain cholesterol levels and neurodegenerative-related protein expression in mice
Authors: Kuo Ping-Hui , Lin Ching-I , Chen Yue-Hwa , Chiu Wan-Chun , Lin Shyh-Hsiang 
Published: April 2015

Ageing accompanied by a decline in cognitive performance may be a result of the long-term effects of oxidative stress on neurologic processes. It has been shown that high-cholesterol contents in the blood and brain may lead to the deposition of the β-amyloid (Aβ) protein in the brain, which damages brain cells. The present study was designed to observe the effect of polyphenol-rich Oriental plums on cognitive function and cerebral neurodegeneration-related protein expression in mice that were fed a high-cholesterol diet for 5 months. The study consisted of four groups: the control (Ctrl) group, which was fed the American Institute of Nutrition (AIN)-93M diet; the high cholesterol (HC) group, which was fed the AIN-93M diet with 5 % cholesterol; the high cholesterol+low Oriental plum (LOP) group, which was fed the AIN-93M diet with 5 % cholesterol and 2 % Oriental plum powder; and the high cholesterol+high Oriental plum (HOP) group, which was fed the AIN-93M diet with 5 % cholesterol and 5 % Oriental plum powder. Measurements of cognitive function were assessed using the Morris water maze, and the mRNA expression of cholesterol hydroxylase (Cyp46),Aβand β-secretase 1 (BACE1) were analysed. The results showed that cholesterol concentrations in both the blood and the brain were significantly higher in the HC group than in the Ctrl and HOP groups at the end of the trial. The high-cholesterol dietper seproduced significant cognitive deficits, which were accompanied by a significantly increased mRNA expression ofCyp46,BACE1,Aβand 24-hydroxycholesterol in the brain cortex and hippocampus. However, all of these variables were non-significantly increased in the HOP group as compared to the Ctrl group. In conclusion, incorporating polyphenol-enriched Oriental plum into a high-cholesterol diet can ameliorate some of the symptoms of neurodegenerative conditions.