bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lebensbedrohliche Einsatzlagen
Authors: Pfenninger E. G. , Hammer T. O. , Holsträter T. , Weiß S. 
Published: July 2024

Zusammenfassung
Hintergrund
Die Gefahr terroristischer Anschläge in der Bundesrepublik Deutschland ist gegeben und nimmt aktuell weiter zu. Öffentlich geförderte Akutkrankenhäuser haben eigenverantwortlich umfassende Vorsorge für ihre Einsatzfähigkeit bei Katastrophen zu treffen. Dieser Auftrag ist auch bei Terror- und Amoklagen sicherzustellen. Eine optimale Abstimmung zwischen präklinischer und klinischer Versorgung ist unabdingbar.

Fragestellung
Es werden Handlungsempfehlungen zur Zusammenarbeit von außerklinischer und klinischer Planung zur Bewältigung eines Massenanfalls von Verletzten bei lebensbedrohlichen Einsatzlagen (LebEL) vorgestellt.

Material und Methoden
Die Klinikübergreifende Sicherheitskonferenz Baden-Württemberg (KLÜSIKO BW) ist eine Arbeitsgemeinschaft aus Vertretern der Akutkliniken in Baden-Württemberg, des Ministeriums für Inneres, Digitalisierung und Migration Baden-Württemberg, des Ministeriums Soziales und Integration Baden-Württemberg, des Landespolizeipräsidiums Baden-Württemberg und der Baden-Württembergischen Krankenhausgesellschaft e. V. Von 2018 bis 2020 wurden in der KLÜSIKO BW Handlungsempfehlungen zur „Zusammenarbeit zwischen Polizei, Kliniken und nichtpolizeilicher Gefahrenabwehr“ erarbeitet. Die Handlungsempfehlungen wurden in 6 Arbeitssitzungen konzertiert und in 2 anschließenden Videokonferenzen parafiert. Eine Empfehlung galt als verabschiedet, wenn abschließend die Vollversammlung der KLÜSIKO BW ihre Zustimmung mit absoluter Mehrheit gab.

Ergebnisse und Diskussion
Zu fordern sind kompetenzbasierte Schnittstellenlösungen für ein reibungsloses Zusammenwirken von Präklinik und Klinik bei der Versorgung von Patienten, die Opfer einer LebEL wurden. Für die Vorplanung wird die Einrichtung einer lokalen Sicherheitskonferenz auf der Ebene Landkreis/Untere Katastrophenschutzbehörde mit folgenden Teilnehmern empfohlen: Untere Katastrophenschutzbehörde, Feuerwehr, regionales Polizeipräsidium, Leitender Notarzt, Rettungsdienste, Katastrophenschutzbeauftragte betroffener Kliniken. Empfohlen wird ein gemeinsames Führungs- und Lagezentrum (FLZ), wo sich Führungspersonal von Polizei, Rettungsdienst, Feuerwehr und Katastrophenschutz einfinden, um die Abwicklung des Schadensereignisses gemeinsam, kompetent und ohne Zeitverlust zu organisieren. Aus diesem FLZ sollten dann die Kliniken in regelmäßigen zeitlichen Abständen mit Informationen versorgt werden. Übungen sollten regelmäßig stattfinden. Eine besondere Bedeutung kommt organisationsübergreifenden Übungen zu.
LC–MS/MS-based phospholipid profiling of plant-pathogenic bacteria with tailored separation of methyl-branched species
Authors: Rudt Edward , Faist Christian , Schwantes Vera , Konrad Nele , Wiedmaier-Czerny Nina , Lehnert Katja , Topman-Rakover Shiri , Brill Aya , Burdman Saul , Hayouka Zvi , Vetter Walter , Hayen Heiko 
Published: July 2024

AbstractPlant-pathogenic bacteria are one of the major constraints on agricultural yield. In order to selectively treat these bacteria, it is essential to understand the molecular structure of their cell membrane. Previous studies have focused on analyzing hydrolyzed fatty acids (FA) due to the complexity of bacterial membrane lipids. These studies have highlighted the occurrence of branched-chain fatty acids (BCFA) alongside normal-chain fatty acids (NCFA) in many bacteria. As several FA are bound in the intact phospholipids of the bacterial membrane, the presence of isomeric FA complicates lipid analysis. Furthermore, commercially available reference standards do not fully cover potential lipid isomers. To address this issue, we have developed a reversed-phase high-performance liquid chromatography (RP-HPLC) method with tandem mass spectrometry (MS/MS) to analyze the phospholipids of various plant-pathogenic bacteria with a focus on BCFA containing phospholipids. The study revealed the separation of three isomeric phosphatidylethanolamines (PE) depending on the number of bound BCFA to NCFA. The validation of the retention order was based on available reference standards in combination with the analysis of hydrolyzed fatty acids through gas chromatography with mass spectrometry (GC/MS) after fractionation. Additionally, the transferability of the retention order to other major lipid classes, such as phosphatidylglycerols (PG) and cardiolipins (CL), was thoroughly examined. Using the information regarding the retention behavior, the phospholipid profile of six plant-pathogenic bacteria was structurally elucidated. Furthermore, the developed LC–MS/MS method was used to classify the plant-pathogenic bacteria based on the number of bound BCFA in the phospholipidome.
Graphical Abstract
Interdisziplinäre Zentren für Autoimmunerkrankungen in Deutschland
Authors: Worm Margitta , Günther Claudia , Claussen Martin , Keyßer Gernot , Kötter Ina , Riemekasten Gabriela , Siegert Elise , Blank Norbert , Sunderkötter Cord , Zeidler Gabriele , Korsten Peter 
Published: July 2024

Zusammenfassung
Hintergrund
Die Betreuung von Patient:innen mit komplexen Autoimmunerkrankungen erfordert eine interdisziplinäre medizinische Versorgung. In Deutschland gibt es zwar eine zunehmende Anzahl von interdisziplinär arbeitenden Zentren für Autoimmunerkrankungen, jedoch sind sie noch nicht flächendeckend vorhanden und ihre Schwerpunkte und interdisziplinären Strukturen häufig nicht nach einem allgemein konsentierten Standard organisiert. Ferner sind sie bislang nicht regelhaft in der allgemeinen Versorgungsstruktur abgebildet.

Ziel der Arbeit
Die Versorgungsstruktur für Autoimmunpatient:innen am Beispiel eines etablierten universitären Zentrums und eines klinischen Falls wird analysiert.

Material und Methoden
Um exemplarisch eine Standortbestimmung interdisziplinär arbeitender Autoimmunzentren in Deutschland durchzuführen, wurden ein Universitätsklinikum für die Strukturanalyse sowie eine Fallvorstellung zur Betrachtung der klinischen Betreuung ausgewählt.

Ergebnisse
In dem ausgewählten Universitätsklinikum werden Patient:innen mit Autoimmunerkrankungen durch Expert*innen verschiedener Fachdisziplinen interdisziplinär betreut. Die Strukturen sind in einem Organigramm verankert. Mithilfe standardisierter Diagnostik- und Therapiepfade („standard operating procedures“ [SOP]) werden Maßnahmen, die für eine jeweils umfassende Diagnostik und Therapie bestimmter Autoimmunerkrankungen fachübergreifend nötig sind, festgelegt. Der von uns vorgestellte Fall stellt anhand einer Patientin mit systemischer Sklerose und Lungenbeteiligung dar, wie ein standardisierter diagnostischer und therapeutischer Pfad in der Praxis umgesetzt werden kann.

Diskussion
Wir diskutieren, welche Maßnahmen fachübergreifend für eine umfassende Diagnostik und Therapie bestimmter Autoimmunerkrankungen notwendig sind, welche Herausforderungen sich bei der Umsetzung ergeben und welche Vorteile sich gegenüber Leitlinien ergeben können – unter anderem weil sie sofort an neue Erkenntnisse angepasst werden können. Die Etablierung eines nationalen Konsenses für den Aufbau, die erforderlichen Strukturen und die Umsetzung in der Patient:innenversorgung innerhalb interdisziplinär arbeitender Zentren für Autoimmunerkrankungen in Deutschland ist wünschenswert.
Cerebrospinal fluid neurofilament light chain in acute optic neuritis and its predictive ability of multiple sclerosis
Authors: Passali Moschoula , Galea Ian , Knudsen Maria Højberg , Lau Laurie Chi , Cramer Stig Præstekjær , Frederiksen Jette Lautrup 
Published: July 2024

Abstract
Background
Studies on the capability of cerebrospinal fluid neurofilament light chain (cNfL) to predict multiple sclerosis (MS) conversion in clinically isolated syndromes have yielded varying results.

Objectives
To expand our understanding of cNfL in optic neuritis (ON) and investigate whether incorporating cNfL into the 2017 McDonald criteria could accelerate the diagnosis of MS in patients with ON.

Methods
cNfL was measured in diagnostic samples from 74 patients with verified ON. MS was diagnosed using the 2017 McDonald criteria with a minimum observation time of two years from ON onset.

Results
20.5% of 44 MS-converters did not fulfil the 2017 McDonald criteria at ON onset. A doubling of cNfL was associated with 207% (74%–514%) higher odds of MS (p = 0.00042, adjusted for age). Fulfilment of ≥ 1 MRI criterion for dissemination in space (DIS) and presence of brain contrast-enhancing lesions were associated with higher cNfL. Furthermore, cNfL correlated with inter-eye differences in retinal nerve fiber layer (RNFL) thickness (Spearman’s ρ = 0.46, p = 8 × 10–5). Incorporating cNfL ≥ 906 pg/mL as a substitute for either dissemination in time or one MRI criterion for DIS increased the sensitivity (90.9% vs. 79.6%) and accuracy (91.9% vs. 87.8%), but also reduced the specificity (93.3% vs. 100%) of the 2017 McDonald criteria.

Conclusion
cNfL was related to MS diagnostic parameters and the degree of RNFL swelling. Clinical use of cNfL may aid in identification of ON patients with increased risk of MS until larger studies have elaborated on the potential loss of specificity if used diagnostically.
The effect of a 4-week, remotely administered, post-exercise passive leg heating intervention on determinants of endurance performance
Authors: John Kevin , Page Joe , Heffernan Shane M. , Conway Gillian E. , Bezodis Neil E. , Kilduff Liam P. , Clark Brad , Périard Julien D. , Waldron Mark 
Published: July 2024

Abstract
Purpose
Post-exercise passive heating has been reported to augment adaptations associated with endurance training. The current study evaluated the effect of a 4-week remotely administered, post-exercise passive leg heating protocol, using an electrically heated layering ensemble, on determinants of endurance performance.

Methods
Thirty recreationally trained participants were randomly allocated to either a post-exercise passive leg heating (PAH, n = 16) or unsupervised training only control group (CON, n = 14). The PAH group wore the passive heating ensemble for 90–120 min/day, completing a total of 20 (16 post-exercise and 4 stand-alone leg heating) sessions across 4 weeks. Whole-body (peak oxygen uptake, gas exchange threshold, gross efficiency and pulmonary oxygen uptake kinetics), single-leg exercise (critical torque and NIRS-derived muscle oxygenation), resting vascular characteristics (flow-mediated dilation) and angiogenic blood measures (nitrate, vascular endothelial growth factor and hypoxia inducible factor 1−α) were recorded to characterize the endurance phenotype. All measures were assessed before (PRE), at 2 weeks (MID) and after (POST) the intervention.

Results
There was no effect of the intervention on test of whole-body endurance capacity, vascular function or blood markers (p > 0.05). However, oxygen kinetics were adversely affected by PAH, denoted by a slowing of the phase II time constant; τ (p = 0.02). Furthermore, critical torque–deoxygenation ratio was improved in CON relative to PAH (p = 0.03).

Conclusion
We have demonstrated that PAH had no ergogenic benefit but instead elicited some unfavourable effects on sub-maximal exercise characteristics in recreationally trained individuals.
Nuclear medicine imaging modalities to detect incidentalomas and their impact on patient management: a systematic review
Authors: Costanzo Roberta , Scalia Gianluca , Strigari Lidia , Ippolito Massimiliano , Paolini Federica , Brunasso Lara , Sciortino Andrea , Iacopino Domenico Gerardo , Maugeri Rosario , Ferini Gianluca , Viola Anna , Zagardo Valentina , Cosentino Sebastiano , Umana Giuseppe E. 
Published: July 2024

Abstract
Purpose
This systematic review aims to investigate the role of nuclear imaging techniques in detecting incidentalomas and their impact on patient management.

Methods
Following PRISMA guidelines, a comprehensive literature search was conducted from February to May 2022. Studies in English involving patients undergoing nuclear medicine studies with incidental tumor findings were included. Data on imaging modalities, incidentaloma characteristics, management changes, and follow-up were extracted and analyzed.

Results
Ninety-two studies involving 64.884 patients were included. Incidentalomas were detected in 611 cases (0.9%), with thyroid being the most common site. PET/CT with FDG and choline tracers showed the highest incidentaloma detection rates. Detection of incidentalomas led to a change in therapeutic strategy in 59% of cases. Various radiotracers demonstrated high sensitivity for incidentaloma detection, particularly in neuroendocrine tumors and prostate cancer.

Conclusion
Nuclear imaging techniques play a crucial role in detecting incidentalomas, leading to significant changes in patient management. The high sensitivity of these modalities highlights their potential in routine oncology follow-up protocols. Future directions may include enhancing spatial resolution and promoting theranostic approaches for improved patient care.
Determination of endometrial cancer molecular subtypes using a whole exome-sequencing based single-method approach
Authors: Mustea Alexander , Ralser Damian J. , Egger Eva K. , Ziehm Ulrike , Vivas Sonia , Brock Stephan , Jackson David , Condic Mateja , Rauschendorf Marc-A. , Würfel Patrick , Dombrowski Frank , Otten Lucia A. , Sun Pengming , Laib Anna , Cordova Miguel Cubas , Hartmann Rahel , Stein Martin A. , Koensgen Dominique , Stope Matthias B. 
Published: July 2024

Abstract
Aim
Endometrial cancer (EC) is heterogeneous with respect to epidemiology, clinical course, histopathology and tumor biology. Recently, The Cancer Genome Atlas (TCGA) network has identified four molecular subtypes with distinct clinical courses by an integrated multi-omics approach. These subtypes are of critical importance in the clinical management of EC. However, determination of TCGA molecular subtypes requires a complex methodological approach that is resource intensive and difficult to implement in diagnostic routine procedures. In this context, Talhouk et al. reported the precise determination of modified subtypes based on molecular surrogates obtained by a two-method approach comprising immunohistochemistry and DNA-sequence analysis (Proactive Molecular Risk Classifier for Endometrial Cancer; ProMisE). In this study, we aimed to identify EC molecular subtypes in analogy to TCGA and ProMisE applying an innovative whole exome-sequencing (WES) based single-method approach.

Methods
WES was performed in a cohort comprising N = 114 EC patients. WES data were analyzed using the oncology treatment decision support software MH Guide (Molecular Health, Heidelberg, Germany) and EC molecular subtypes in analogy to TCGA and ProMisE were determined. Results from both classifications were compared regarding their prognostic values using overall survival and progression-free survival analyses.

Results
Applying a single-method WES-approach, EC molecular subtypes analogue to TCGA and ProMisE were identified in the study cohort. The surrogate marker-analogue classification precisely identified high-risk and low-risk EC, whereas the TCGA-analogue classification failed to obtain significant prognostic values in this regard.

Conclusion
Our data demonstrate that determination of EC molecular subtypes analogue to TCGA and ProMisE is feasible by using a single-method WES approach. Within our EC cohort, prognostic implications were only reliably provided by applying the surrogate marker-analogue approach. Designation of molecular subtypes in EC will be increasingly important in routine clinical practice. Thus, the single-method WES approach provides an important simple tool to tailor therapeutic decisions in EC.
Einfluss unterschiedlicher Ansätze zur Generierung von Ausfallzeitreihen auf die Kenngrößen zur Resource Adequacy
Authors: Pacco Kevin , Moser Albert 
Published: July 2024

ZusammenfassungAufgrund der strukturellen Veränderungen im europäischen Stromsystem gewinnt die Sicherheit der Stromversorgung in aktuellen Forschungsfragen zunehmend an Bedeutung. Um sicherzustellen, dass die Stromversorgungssicherheit dadurch nicht beeinträchtigt wird, ist eine Bewertung der Angemessenheit der Ressourcen (Resource Adequacy) erforderlich, um festzustellen, ob das Elektrizitätsversorgungssystem über ausreichende Ressourcen verfügt, um die zukünftige Nachfrage nach elektrischer Energie zu decken. Zur Untersuchung der Resource Adequacy wird hierbei ein probabilistisches Modell verwendet. Hierbei gehen insbesondere Nicht-Verfügbarkeiten von Ressourcen als Eingangsdatum ein. In diesem Kontext finden neben klimaabhängigen Unsicherheiten (hydraulische Zuflüsse, Windgeschwindigkeit, Sonneneinstrahlungen, etc.) auch insbesondere (un-)geplante Ausfälle von Erzeugungsanlagen Berücksichtigung. Zur Generierung von Ausfallzeitreihen sind unterschiedliche Ansätze sowie zusätzlich unterschiedliche Parametrierungen dieser Ansätze möglich. Vor diesem Hintergrund stellt sich die Frage, welchen Einfluss diese auf die Kenngrößen zur Resource Adequacy haben. Ziel dieser Arbeit ist es, ein Verfahren zur Ermittlung von Ausfallzeitreihen zu entwickeln. Im Anschluss werden die Einflüsse unterschiedlicher Ausprägungen dieses Verfahrens untersucht und quantifiziert.
MASCC/ISOO clinical practice statement: Current understanding on controversies in basic oral care in hemato-oncology and hematopoietic cell transplantation
Authors: Raber-Durlacher Judith E. , Zadik Yehuda , Treister Nathaniel S. , Stempler Noa , Bruno Julia S. , Epstein Joel B. , Elad Sharon 
Published: July 2024

Abstract
Purpose
A MASCC/ISOO Clinical Practice Statement (CPS) is aimed at generating a concise tool for clinicians, which concentrates on practical information needed for the management of oral complications of cancer patients. This CPS is focused on the current understanding of controversies that may arise while providing basic oral care in hemato-oncology patients and hematopoietic cell transplantation recipients (HCT). The CPS will summarize and elucidate controversies that have appeared in the literature and professional discussions.

Methods
This CPS was developed based on a critical evaluation of the literature followed by a structured discussion of a group of leading experts, members of the Oral Care Study Group of MASCC/ISOO. The information is presented in the form of succinct bullets to generate a short manual about the best standard of care.

Results
Controversies about the use of chlorhexidine (CHX) oral rinse, mechanical dental plaque removal procedures, the need for toothbrush replacement during phases of low blood cell counts, and the use of lidocaine mouthwash for oral pain were identified and discussed. Consensus about the best standard of care was outlined.

Conclusion
The following ratifications are applicable for oral care in hemato-oncology patients and patients undergoing HCT: (1) CHX may reduce the risk of oral infections, although it was not found to reduce the risk of oral mucositis. (2) Toothbrushing and proficient interproximal cleaning should not be discouraged during HCT. (3) Toothbrushes do not need to be replaced daily and are preferred over cleansing swabs. (4) Lidocaine rinse, swish and spit, may be considered to palliate oral mucosal pain if applied in a certain manner.
Screen-printed electrode-based sensor for rapid ketamine determination: optimization and on-site application for seized drugs analysis
Authors: Stelmaszczyk Paweł , Białkowska Katarzyna , Sekuła Karolina , Stanaszek Roman , Wietecha-Posłuszny Renata 
Published: July 2024

AbstractThis study investigates the electrochemical behavior of ketamine using an in-lab fabricated screen-printed electrode system and explores its potential application in quantitative analysis. Cyclic voltammetry and differential pulse voltammetry (DPV) experiments were employed to characterize the oxidation behavior of ketamine. Systematic optimization of DPV parameters, including pulse amplitude, pulse width, potential step, potential, and time accumulation for analyte preconcentration resulted in the selection of optimal conditions for quantitative analysis. The developed DPV method exhibited excellent linearity (R2 = 0.996) over the concentration range of 50–500 µM, with a limit of detection of 15 µM and a limit of quantification of 50 µM. Authentic samples analysis demonstrated the utility of the proposed sensor for quantitative analysis of ketamine in pharmaceutical products and seized drug samples. Overall, the developed sensor offers a promising tool for the rapid and accurate analysis of ketamine in various samples with potential applications in on-site forensic analysis.
Graphical abstract
Probability density function of roll amplitude for parametric rolling using moment equation
Authors: Maruyama Yuuki , Maki Atsuo , Dostal Leo 
Published: July 2024

AbstractFor the ship stability estimation and ship design, it is helpful to know the probability of the roll amplitude exceeding a certain threshold. Therefore, it is necessary to obtain the probability density function of the roll amplitude. In this study, first, we derive the moment values of roll amplitude by combining the moment equations and the linearity of expectation. With this, we propose a method to estimate the probability density function of the roll amplitude by using the obtained moment values. The results of our proposed method are compared to those obtained from Monte Carlo simulations. When the higher-order cumulant neglect closure method is used, the moment values resulting from the moment equations are close to the results of corresponding Monte Carlo simulations. In addition, our proposed method for deriving the probability density function of the roll amplitude is validated by comparison with Monte Carlo simulation results. In conclusion, we can state that the proposed methods for deriving the moments and the probability density function of the roll amplitude are available for practical use cases.
Single-cell sequencing reveals novel proliferative cell type: a key player in renal cell carcinoma prognosis and therapeutic response
Authors: Ye Bicheng , Ji Hongsheng , Zhu Meng , Wang Anbang , Tang Jingsong , Liang Yong , Zhang Qing 
Published: July 2024

AbstractRenal cell carcinoma (RCC) is characterized by a variety of subtypes, each defined by unique genetic and morphological features. This study utilizes single-cell RNA sequencing to explore the molecular heterogeneity of RCC. A highly proliferative cell subset, termed as “Prol,” was discovered within RCC tumors, and its increased presence was linked to poorer patient outcomes. An artificial intelligence network, encompassing traditional regression, machine learning, and deep learning algorithms, was employed to develop a Prol signature capable of predicting prognosis. The signature demonstrated superior performance in predicting RCC prognosis compared to other signatures and exhibited pan-cancer prognostic capabilities. RCC patients with high Prol signature scores exhibited resistance to targeted therapies and immunotherapies. Furthermore, the key gene CEP55 from the Prol signature was validated by both proteinomics and quantitative real time polymerase chain reaction. Our findings may provide new insights into the molecular and cellular mechanisms of RCC and facilitate the development of novel biomarkers and therapeutic targets.