bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircARAP2 controls sMICA-induced NK cell desensitization by erasing CTCF/PRC2-induced suppression in early endosome marker RAB5A
Authors: Guo Feifei , Du Nawen , Wen Xue , Li Zhaozhi , Guo Yantong , Zhou Lei , Hoffman Andrew R. , Li Lingyu , Hu Ji-Fan , Cui Jiuwei 
Published: July 2024

AbstractNatural killer cells (NK) are the "professional killer" of tumors and play a crucial role in anti-tumor immunotherapy. NK cell desensitization is a key mechanism of tumor immune escape. Dysregulated NKG2D-NKG2DL signaling is a primary driver of this desensitization process. However, the factors that regulate NK cell desensitization remain largely uncharacterized. Here, we present the first report that circular RNA circARAP2 (hsa_circ_0069396) is involved in the soluble MICA (sMICA)-induced NKG2D endocytosis in the NK cell desensitization model. CircARAP2 was upregulated during NK cell desensitization and the loss of circARAP2 alleviated NKG2D endocytosis and NK cell desensitization. Using Chromatin isolation by RNA purification (ChIRP) and RNA pull-down approaches, we identified that RAB5A, a molecular marker of early endosomes, was its downstream target. Notably, transcription factor CTCF was an intermediate functional partner of circARAP2. Mechanistically, we discovered that circARAP2 interacted with CTCF and inhibited the recruitment of CTCF-Polycomb Repressive Complex 2 (PRC2) to the promoter region of RAB5A, thereby erasing histone H3K27 and H3K9 methylation suppression to enhance RAB5A transcription. These data demonstrate that inhibition of circARAP2 effectively alleviates sMICA-induced NKG2D endocytosis and NK cell desensitization, providing a novel target for therapeutic intervention in tumor immune evasion.
Joint Inversion of DC and TEM Methods for Geological Imaging
Authors: Bortolozo Cassiano Antonio , Campaña Julian David Realpe , Santos Fernando Acácio Monteiro Dos , De Oliveira Gabriela Serejo , Porsani Jorge Luís , Pryer Tristan , Sialounas Georgios 
Published: July 2024

AbstractIn this study, we present a new methodology for 2D joint inversion, or data fusion, of DC electro-resistivity and Transient Electromagnetic methods. These geophysical techniques have traditionally been used separately, but by combining them, we aim to decrease ambiguities and increase the robustness of the resulting subsurface model. The inversion process was conducted using the classical Occam method with smooth models and synthetic studies were also conducted to understand the limitations and advantages of the method. We also applied the algorithm to data obtained in groundwater exploration in Brazil, and the results showed that the 2D joint inversion is promising in increasing accuracy and reducing ambiguity in subsurface imaging.
Safe and reconfigurable manufacturing: safety aware multi-agent control for Plug & Produce system
Authors: Massouh Bassam , Danielsson Fredrik , Lennartson Bengt , Ramasamy Sudha , Khabbazi Mahmood 
Published: July 2024

AbstractPlug & Produce aims to revolutionize manufacturing by enabling seamless machine integration into production processes without extensive programming. This concept, leveraging multi-agent systems (MAS), offers increased flexibility and faster production ramp-up times after reconfiguration. As automated manufacturing moves towards greater human integration, this paper addresses safe operation within the Plug & Produce concept. The main safety challenge arises from autonomous decision-making, as agents in the MAS lack awareness of the risk consequences of their behavior. Additionally, the difficulty of perceiving the system’s exact behavior leads to the implementation of overly restrictive safety measures. This limits the system’s flexibility and ability to make decisions for efficient production. This paper proposes a method utilizing multi-agent control to conduct automatic safety analysis and reason task allocations to avoid risks. The method’s benefits are the generation of control actions that comply with safety requirements during operation, eliminating the need for overly restrictive safety measures and allowing more effective equipment utilization. The method’s benefit is illustrated through a manufacturing scenario with two different configurations: one using a hazardous machine and the other using a less hazardous one. Formal verification using the model checker NuSMV demonstrated that safety requirements were satisfied in both configurations, without the need for manual modifications of the safety control system after reconfiguration. The results for this specific manufacturing scenario showed that there are more reachable states (20 states) in the safer second configuration, compared to the first configuration (16 states). This means that the presented control strategy dynamically adjusts the system’s behavior to confirm safety. Hence, this method maintains safety without fixed safety rules that limit the operations.
The system dynamics approach for a global evolutionary analysis of sustainable development
Authors: Feder Christophe , Callegari Beniamino , Collste David 
Published: July 2024

AbstractThe challenge of pursuing sustainable development highlights the relevance of the complex mechanisms through which natural and social selection processes affect and are affected by the economic system. Current economic development is unsustainable because it fails to generate long-term systemic compatibility between firms and their natural and social environment. This paper evaluates the issue from an evolutionary perspective by conceptualising unsustainability as the emergence of negative macro-selection effects, arising from both the natural and social domains, and argues for a methodological need for closer integration of system dynamics modelling within the evolutionary field. The Earth4All model is then used to illustrate the complex interactions between economic, social, and natural selection processes. The model results illustrate that the current global development trajectory is strongly unsustainable from both a natural and a social perspective, leading to the emergence of relevant natural and social macro-selection mechanisms, whose systemic interactions bring further complex adverse effects.
Eulerian and Lagrangian Stability in Zeitlin’s Model of Hydrodynamics
Authors: Modin Klas , Perrot Manolis 
Published: July 2024

AbstractThe two-dimensional (2-D) Euler equations of a perfect fluid possess a beautiful geometric description: they are reduced geodesic equations on the infinite-dimensional Lie group of symplectomorphims with respect to a right-invariant Riemannian metric. This structure enables insights to Eulerian and Lagrangian stability via sectional curvature and Jacobi equations. The Zeitlin model is a finite-dimensional analogue of the 2-D Euler equations; the only known discretization that preserves the rich geometric structure. Theoretical and numerical studies indicate that Zeitlin’s model provides consistent long-time behaviour on large scales, but to which extent it truly reflects the Euler equations is mainly open. Towards progress, we give here two results. First, convergence of the sectional curvature in the Euler–Zeitlin equations on the Lie algebra $$\mathfrak {su}(N)$$

su
(
N
)

to that of the Euler equations on the sphere. Second, $$L^2$$

L
2

-convergence of the corresponding Jacobi equations for Lagrangian and Eulerian stability. The results allow geometric conclusions about Zeitlin’s model to be transferred to Euler’s equations and vice versa, which could expedite the ultimate aim: to characterize the generic long-time behaviour of perfect 2-D fluids.
Impact of Exposure to Leaves From Metal-Polluted Sites on the Developmental Parameters of Larvae of the Dark Sword-Grass, Agrotis ipsilon (Lepidoptera: Noctuidae)
Authors: Rasmy Shrouk , Mohamed Amr , Yousef Hesham A. 
Published: July 2024

AbstractInsects are impacted by pollutants in their environments and food sources. Herein, we set out a semi-field study to assess the impact of environmental heavy metal contamination on developmental parameters, energy reserves, and acidic and alkaline phosphatases in the larval Agrotis ipsilon (Lepidoptera: Noctuidae). Castor leaves from contaminated and uncontaminated (reference site) areas were fed to A. ipsilon larvae in all treatments. The heavy metal concentrations in the plant from different areas (contaminated and reference sites) and in the larvae were analyzed. Toxic effects were observed in the larvae feeding on the leaves from the metal contaminated areas. Larval and pupal weights, growth indices, and larval fitness were all significantly lower than in the reference group. Likewise, in the third and fourth instars, there was a significant decrease in both the survival and moth emergence rates. In contrast, the pupation duration was significantly longer. Total protein, lipid, and glycogen content showed significant reductions in treated larvae. Larval homogenate samples contaminated with heavy metals showed a significant increase in acid- and alkaline- phosphatase levels. The results obtained could provide a basis for a long-term evaluation of the risk associated with heavy metals and their impact on plant populations and important agricultural pests.
Construction of an expression platform for fungal secondary metabolite biosynthesis in Penicillium crustosum
Authors: Zhou Jenny , Chen Xiaoling , Li Shu-Ming 
Published: July 2024

Abstract
Filamentous fungi are prolific producers of bioactive natural products and play a vital role in drug discovery. Yet, their potential cannot be fully exploited since many biosynthetic genes are silent or cryptic under laboratory culture conditions. Several strategies have been applied to activate these genes, with heterologous expression as one of the most promising approaches. However, successful expression and identification of new products are often hindered by host-dependent factors, such as low gene targeting efficiencies, a high metabolite background, or a lack of selection markers. To overcome these challenges, we have constructed a Penicillium crustosum expression host in a pyrG deficient strain by combining the split-marker strategy and CRISPR-Cas9 technology. Deletion of ligD and pcribo improved gene targeting efficiencies and enabled the use of an additional selection marker in P. crustosum. Furthermore, we reduced the secondary metabolite background by inactivation of two highly expressed gene clusters and abolished the formation of the reactive ortho-quinone methide. Finally, we replaced the P. crustosum pigment gene pcr4401 with the commonly used Aspergillus nidulans wA expression site for convenient use of constructs originally designed for A. nidulans in our P. crustosum host strain. As proof of concept, we successfully expressed a single polyketide synthase gene and an entire gene cluster at the P. crustosum wA locus. Resulting transformants were easily detected by their albino phenotype. With this study, we provide a highly efficient platform for heterologous expression of fungal genes.

Key points

Construction of a highly efficient Penicillium crustosum heterologous expression host

Reduction of secondary metabolite background by genetic dereplication strategy

Integration of wA site to provide an alternative host besides Aspergillus nidulans

Graphical Abstract
Extrapulmonary hyalinizing granuloma: a rare case with intra-articular and tenosynovial involvement
Authors: Alhumayed Mohanad , Austin Ryan P. , Chang Eric Y. 
Published: July 2024

AbstractExtrapulmonary hyalinizing granuloma (EPHG) is a notably rare condition, representing an exaggerated chronic immune response to antigenic stimuli. This report presents the first documented case of intra-articular and tenosynovial EPHG with radiological evaluation and pathological confirmation in a 60-year-old man presenting with wrist pain and swelling. Imaging findings were relatively symmetric with marked distension of the distal radioulnar joints and extensor tendon sheaths with masses and nodules of various sizes surrounded by synovitis and accompanied by bony erosions. On US, the masses were heterogeneous but mostly hypo- to iso-echoic compared to muscle and relatively hypovascular. On MRI, compared to muscle, the nodules exhibited iso-intense signal on T1-weighted images, iso- to mildly hyper-intense signal on T2-weighted fat-suppressed images, and minimal enhancement on post-contrast images. The diagnosis of EPHG was revealed through biopsy and pathologic examination with glucocorticoids being effective in treatment.
Comparison of the tolerability of 161Tb- and 177Lu-labeled somatostatin analogues in the preclinical setting
Authors: Busslinger Sarah D. , Mapanao Ana Katrina , Kegler Kristel , Bernhardt Peter , Flühmann Fabienne , Fricke Julia , Zeevaart Jan Rijn , Köster Ulli , van der Meulen Nicholas P. , Schibli Roger , Müller Cristina 
Published: July 2024

Abstract
Purpose
[177Lu]Lu-DOTATATE is an established somatostatin receptor (SSTR) agonist for the treatment of metastasized neuroendocrine neoplasms, while the SSTR antagonist [177Lu]Lu-DOTA-LM3 has only scarcely been employed in clinics. Impressive preclinical data obtained with [161Tb]Tb-DOTA-LM3 in tumor-bearing mice indicated the potential of terbium-161 as an alternative to lutetium-177. The aim of the present study was to compare the tolerability of 161Tb- and 177Lu-based DOTA-LM3 and DOTATATE in immunocompetent mice.

Methods
Dosimetry calculations were performed based on biodistribution data of the radiopeptides in immunocompetent mice. Treatment-related effects on blood cell counts were assessed on Days 10, 28 and 56 after application of [161Tb]Tb-DOTA-LM3 or [161Tb]Tb-DOTATATE at 20 MBq per mouse. These radiopeptides were also applied at 100 MBq per mouse and the effects compared to those observed after application of the 177Lu-labeled counterparts. Bone marrow smears, blood plasma parameters and organ histology were assessed at the end of the study.

Results
The absorbed organ dose was commonly higher for the SSTR antagonist than for the SSTR agonist and for terbium-161 over lutetium-177. Application of a therapeutic activity level of 20 MBq [161Tb]Tb-DOTA-LM3 or [161Tb]Tb-DOTATATE was well tolerated without major hematological changes. The injection of 100 MBq of the 161Tb- and 177Lu-based somatostatin analogues affected the blood cell counts, however. The lymphocytes were 40–50% lower in treated mice compared to the untreated controls on Day 10 irrespective of the radionuclide employed. At the same timepoint, thrombocyte and erythrocyte counts were 30–50% and 6–12% lower, respectively, after administration of the SSTR antagonist (p < 0.05) while changes were less pronounced in mice injected with the SSTR agonist. All blood cell counts were in the normal range on Day 56. Histological analyses revealed minimal abnormalities in the kidneys, liver and spleen of treated mice. No correlation was observed between the organ dose and frequency of the occurrence of abnormalities.

Conclusion
Hematologic changes were more pronounced in mice treated with the SSTR antagonist than in those treated with the SSTR agonist. Despite the increased absorbed dose delivered by terbium-161 over lutetium-177, [161Tb]Tb-DOTA-LM3 and [161Tb]Tb-DOTATATE should be safe at activity levels that are recommended for their respective 177Lu-based analogues.

Graphical Abstract
Anconeus and pronation: a palpatory and ultrasonographic study
Authors: Canoso Juan J. , Murillo-González Jorge , Mérida-Velasco José Ramón , Kalish Robert A. , Olivas-Vergara Otto , Gómez-Moreno Cristina , Blas Eva García-Carpintero , Fuensalida-Novo Gema , Naredo Esperanza 
Published: July 2024

Abstract
Purpose
Depending on its axis, pronation varies from the radius rotation around the steady ulna to the reciprocal adduction of the radius and abduction of the ulna. While there is no question that pronator teres is a central pronation agonist, anconeus’s role is not settled. The current investigation comparing palpation and ultrasonography in these two muscles during pronation along the axis capitulum-second digit evolved from a serendipitous finding in a clinical anatomy seminar.

Methods
Single-hand palpation and two-transducer ultrasonography over anconeus and pronator teres were used on ten normal subjects to investigate their contraction during pronation around the capitulum-second digit axis. These studies were done independently and blind to the results of the other. The statistical analysis between palpation and ultrasonography was performed with Cohen’s kappa coefficient and the χ2 test.

Results
On palpation, on resisted full pronation, anconeus contracted in 8/10 subjects and pronator teres in 10/10 subjects. Without resistance, the corresponding ratios were 5/10 and 9/10. On two-transducer ultrasonography, the comparable ratios were 7/10 and 10/10, and 3/10 and 10/10. A fair concordance (Cohen’s kappa = 0.21) between palpation and ultrasonography in detecting the simultaneous status of anconeus and pronator teres during resisted full pronation. Anatomic dissection illustrated the elements involved.

Conclusions
Plain palpation confirmed by ultrasonography showed the simultaneous contraction of anconeus and pronator teres during resisted pronation in most of the studied subjects. The study suggests that palpation can be helpful in directly studying muscle activity during movement.
Superior detection of low-allele burden Janus kinase 2 V617F mutation and monitoring clonal evolution in myeloproliferative neoplasms using chip-based digital PCR
Authors: Lu Yiyi , Lin Lin , Lin Jiafei , Wu Beiying , Cai Gang , Wang Xuefeng , Ma Xuefei 
Published: July 2024

AbstractThe JAK2 V617F is a prevalent driver mutation in Philadelphia chromosome-negative myeloproliferative neoplasms (Ph−MPNs), significantly affecting disease progression, immunophenotype, and patient outcomes. The World Health Organization (WHO) guidelines highlight the JAK2 V617F mutation as one of the key diagnostic criterions for Ph−MPNs. In this study, we analyzed 283 MPN samples with the JAK2 V617F mutation to assess the effectiveness of three detection technologies: chip-based digital PCR (cdPCR), real-time quantitative PCR (qPCR), and next-generation sequencing (NGS). Additionally, we investigated the relationship between JAK2 V617F mutant allele burden (% JAK2 V617F) and various laboratory characteristics to elucidate potential implications in MPN diagnosis. Our findings demonstrated high conformance of cdPCR with qPCR/NGS for detecting % JAK2 V617F, but the mutant allele burdens detected by qPCR/NGS were lower than those detected by cdPCR. Moreover, the cdPCR exhibited high sensitivity with a limit of detection (LoD) of 0.08% and a limit of quantification (LoQ) of 0.2% for detecting % JAK2 V617F in MPNs. Clinical implications were explored by correlating % JAK2 V617F with various laboratory characteristics in MPN patients, revealing significant associations with white blood cell counts, lactate dehydrogenase levels, and particularly β2-microglobulin (β2-MG) levels. Finally, a case report illustrated the application of cdPCR in detecting low-allele burdens in a de novo chronic myeloid leukemia (CML) patient with a hidden JAK2 V617F subclone, which expanded during tyrosine kinase inhibitor (TKI) treatment. Our findings underscore the superior sensitivity and accuracy of cdPCR, making it a valuable tool for early diagnosis and monitoring clonal evolution.
Robustness of radiomics among photon-counting detector CT and dual-energy CT systems: a texture phantom study
Authors: Zhu Lan , Dong Haipeng , Sun Jing , Wang Lingyun , Xing Yue , Hu Yangfan , Lu Junjie , Yang Jiarui , Chu Jingshen , Yan Chao , Yuan Fei , Zhong Jingyu 
Published: July 2024

Abstract

Objectives
To evaluate the robustness of radiomics features among photon-counting detector CT (PCD-CT) and dual-energy CT (DECT) systems.

Methods
A texture phantom consisting of twenty-eight materials was scanned with one PCD-CT and four DECT systems (dual-source, rapid kV-switching, dual-layer, and sequential scanning) at three dose levels twice. Thirty sets of virtual monochromatic images at 70 keV were reconstructed. Regions of interest were delineated for each material with a rigid registration. Ninety-three radiomics were extracted per PyRadiomics. The test-retest repeatability between repeated scans was assessed by Bland-Altman analysis. The intra-system reproducibility between dose levels, and inter-system reproducibility within the same dose level, were evaluated by intraclass correlation coefficient (ICC) and concordance correlation coefficient (CCC). Inter-system variability among five scanners was assessed by coefficient of variation (CV) and quartile coefficient of dispersion (QCD).

Results
The test–retest repeatability analysis presented that 97.1% of features were repeatable between scan–rescans. The mean ± standard deviation ICC and CCC were 0.945 ± 0.079 and 0.945 ± 0.079 for intra-system reproducibility, respectively, and 86.0% and 85.7% of features were with ICC > 0.90 and CCC > 0.90, respectively, between different dose levels. The mean ± standard deviation ICC and CCC were 0.157 ± 0.174 and 0.157 ± 0.174 for inter-system reproducibility, respectively, and none of the features were with ICC > 0.90 or CCC > 0.90 within the same dose level. The inter-system variability suggested that 6.5% and 12.8% of features were with CV < 10% and QCD < 10%, respectively, among five CT systems.

Conclusion
The radiomics features were non-reproducible with significant variability in values among different CT techniques.

Clinical relevance statement
Radiomics features are non-reproducible with significant variability in values among photon-counting detector CT and dual-energy CT systems, necessitating careful attention to improve the cross-system generalizability of radiomic features before implementation of radiomics analysis in clinical routine.

Key Points

CT radiomics stability should be guaranteed before the implementation in the clinical routine.

Radiomics robustness was on a low level among photon-counting detectors and dual-energy CT techniques.

Limited inter-system robustness of radiomic features may impact the generalizability of models.
Gas detection using cavity-enhanced Raman spectroscopy with a bidirectional multi-pass cell and polarization beam-splitting optical path
Authors: Zheng Yuhao , Zou Xiaer , He Sailing 
Published: July 2024

AbstractWe demonstrate a substantial enhancement of gas Raman scattering using a bidirectional multi-pass cavity CERS system, which incorporates a polarization beam-splitting optical path. The system design allows the laser light to traverse the multi-pass cavity for four specific trips, satisfying the need for quick detection of various gas components. Our gas detection experiments using multi-pass cavities with different times of reflection indicate that the addition of polarization beam-splitting optical path gives 1.5 to 1.68 times enhancement of Raman signal compared with that of the system without polarization beam-splitting. For the detection of CH4, a limit of detection of 1.66 ppm was achieved with our system using a multi-pass cell with 41 times of reflection and an integration time of 30s. Our proposed design, which integrates a bidirectional multi-pass cavity with polarization beam-splitting optical path, gives an economical multicomponent gas detection system and a valuable tool for guiding the design and precise alignment of these cavities. This system shows significant promise for applications in e.g. human breath and environmental monitoring.