bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring Novel Coumarin‐Tethered Bis‐Triazoles: Apoptosis Induction in Human Pancreatic Cancer Cells, Antimicrobial Effects, and Molecular Modelling Investigations
Authors: Dhawan Sanjeev , Singh Ashona , Manhas Neha , Seboletswe Pule , Khubone Lungisani , Kumar Gobind , Jonnalagadda Sreekantha B. , Raza Asif , Sharma Arun K. , Singh Parvesh 
Published: July 2024

AbstractIn the present study, we identified that two representative compounds (7 c and 9 f) of our newly synthesized coumarin‐tagged bis‐triazoles induced apoptosis in human pancreatic cells (PANC‐1) by caspase 3/7mediated pathway. Both 7 c and 9 f (IC50=7.15±1.19 and 6.09±0.79 μM, respectively) were found to be ~100 times superior against PANC‐1 as compared to the standard drug Gemcitabine (IC50=>500 μM), without showing any toxicity to the normal pancreatic epithelial cells (H6C7). Molecular docking studies further endorsed them as potential pancreatic cancer therapeutics due to their strong hydrogen bonding interactions with the epidermal growth factor receptor (EGFR) enzyme, which is overexpressed in cancerous cells including pancreatic cancer. Additionally, these compounds also showed moderate inhibitory activity against a panel of microbial strains. Overall, our findings reveal that the coumarin hybrids 7 c and 9 f are viable chemotypes to be adopted as templates for the development of new anticancer drugs, particularly against pancreatic cancer.
Pre‐Sealing of Endodontic Access Cavities for the Preservation of Anterior Teeth Fracture Resistance
Authors: Shafiei Fereshteh , Tavangar Maryam S. 
Published: July 2024

ABSTRACTObjectiveSodium hypochlorite solution (NaOCl) is an effective canal irrigant but interferes with the mechanical features of dentin and the bonding capability of adhesives when restoring endodontically treated teeth. This study evaluated whether access cavity resin sealing before using canal irrigant would augment the resistance of endodontically treated anterior teeth against fracture.MethodsSixty maxillary incisors underwent endodontic treatment in five groups (n = 12). Irrigation with 5.25% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA) was performed in all groups except for Group 5. After root canal obturation, in Group 1, the access cavity was kept unrestored. In Group 2, immediate restoration after obturation was achieved. For Group 3, delayed restoration after 1 week was provided. In Group 4 (pre‐sealed), before canal irrigation, the dentin surface of access cavities was sealed using self‐adhesive composite resin (Vertise Flow) and then restored after obturation. In Group 5, which was saline irrigated, immediate restoration was performed. After storage and thermal cycling for 5000 cycles at 5°C–55°C with a dwell time of 15 s and a transfer time of 5 s, teeth were statically loaded by a universal testing machine until a fracture occurred. Data were collected as the fracture resistance (FR) and analyzed using the one‐way analysis of variance and Tukey's tests.ResultsFR significantly differed between all groups (p < 0.001). The lowest FR was recorded in the unrestored group (284 ± 86 N), which was not statistically different from the immediately restored group (p = 0.065). The pre‐sealed group exhibited the highest FR value (810 ± 127 N, p ≤ 0.02 vs. other groups). The FR of the saline‐irrigated and delayed restored groups was almost similar (p = 0.13).ConclusionsNaOCl/EDTA irrigation resulted in an adverse effect on FR. Delayed restoration could reduce this adverse effect. Access cavity pre‐sealing with flowable composites led to a higher FR than conventional methods and may be considered an effective step during treatment procedures.
Biodiversity in changing environments: An external‐driver internal‐topology framework to guide intervention
Authors: Suding Katharine N. , Collins Courtney G. , Hallett Lauren M. , Larios Loralee , Brigham Laurel M. , Dudney Joan , Farrer Emily C. , Larson Julie E. , Shackelford Nancy , Spasojevic Marko J. 
Published: July 2024

AbstractAccompanying the climate crisis is the more enigmatic biodiversity crisis. Rapid reorganization of biodiversity due to global environmental change has defied prediction and tested the basic tenets of conservation and restoration. Conceptual and practical innovation is needed to support decision making in the face of these unprecedented shifts. Critical questions include: How can we generalize biodiversity change at the community level? When are systems able to reorganize and maintain integrity, and when does abiotic change result in collapse or restructuring? How does this understanding provide a template to guide when and how to intervene in conservation and restoration? To this end, we frame changes in community organization as the modulation of external abiotic drivers on the internal topology of species interactions, using plant–plant interactions in terrestrial communities as a starting point. We then explore how this framing can help translate available data on species abundance and trait distributions to corresponding decisions in management. Given the expectation that community response and reorganization are highly complex, the external‐driver internal‐topology (EDIT) framework offers a way to capture general patterns of biodiversity that can help guide resilience and adaptation in changing environments.
Optimal configuration of low‐grade waste heat driven seawater desalination using data envelopment analysis: A case study of industrial application
Authors: Yousefi Paria , Avami Akram 
Published: July 2024

AbstractWater supply challenges are caused by population growth, industrialization, as well as the scarcity of freshwater resources. Low‐grade waste heat‐driven seawater desalination technologies may improve the water‐energy nexus issues of desalination systems by different configurations. The data envelopment analysis is used to determine the best final decision to achieve a better comparison of different parameters. The optimal configuration of low‐grade waste heat driven seawater desalination is studied using flue gas waste heat in heat recovery boilers of an industrial oil refinery. Nine different types of multistage flash and multi‐effect distillation (MED) desalination plants have been considered. The results show that the multistage flash brine recirculation may produce more desalinated water while the multi‐effect distillation with three stages (3‐stage MED) has the best payback period. Using 3‐stage MED with a production of approximately 19,630 kg/h of water from 5.3 MW exhaust gas is more suitable for this design. Thus, the proposed strategy guides us toward the best decisions to configure low‐grade waste heat‐driven desalination plants for better design considering rigorous simulations for the plants. Moreover, this framework enables us to consider different criteria of technical, economic, and environmental issues for optimal configuration.
Drying behavior and nutritional quality of bitter‐gourd slices dried in a solar dryer with tray and skewer arrangement
Authors: Borse Sudarshan M. , Singh Manpreet , Kaur Preetinder , Singh Sukhmeet , Zalpouri Ruchika 
Published: July 2024

AbstractIn this investigation, we assessed the efficacy of an indirect‐type solar drying system that utilized skewers and rack arrangements, comparing it to a conventional drying cabinet equipped with trays for dehydrating bitter gourd slices. We examined the influence of pretreatment methods and loading quantities on the solar drying process for bitter gourd slices in both configurations. The drying behavior of various combinations was scrutinized, and the physicochemical attributes of the dried bitter gourd samples, including total phenolic content, total flavonoid content, total chlorophyll content, antioxidant capacity, ascorbic acid content, and color, were analyzed. Rehydration characteristics, such as rehydration ratio, coefficient of rehydration, percent water in the rehydrated sample, and hardness of the rehydrated sample, were also determined. The bitter gourd slices achieved a final moisture content of 6.84%–8.27% wb after drying from an initial range of 88%–90% wb, within a total elapsed time of 28–55 h. The solar drying cabinet utilizing skewers exhibited enhanced efficiency, featuring reduced drying time and superior product quality compared to the tray‐equipped drying cabinet. This improved performance is ascribed to enhanced hot air circulation over the produce surface, facilitated by the uniform spacing between skewers on racks within the drying chamber.
Comparative energy metrics and annual efficiency analyses of CPC‐ETC integrated single slope solar desalting unit
Authors: Singh Ajay Raj , Agarwal Alka Bani , Singh Desh Bandhu 
Published: July 2024

AbstractThis paper deals with the investigation of N identical compound parabolic concentrator evacuated tubular collector included single slope solar desalting unit (N‐CPC‐ETC‐SSU) by incorporating energy metrics for solving contemporary issue of water scarcity in the society. It will contribute to the sustainable development of society and recede the dependency on fossil fuels, too. The energy metrics investigation is important for energy system because it talks about the feasibility of the system. The methodology consists of getting fundamental equations from energy balance equations. All fundamental equations with relevant data are fed to the code developed in MATLAB followed by the computation of overall energy and exergy. All types of climatic situations have been considered for the analysis. Data required for the same are accessed from IMD, Pune, India. The energy payback period, energy production factor, and life cycle conversion efficiency for N‐CPC‐ETC‐SSU have been calculated at eight number of collectors, 0.012 mass flow rate and 0.14 m water depth. Results of N‐CPC‐ETC‐SSU have been compared with results of SSU included with ETCs. Concludingly, energy payback period is lower by 6.41%, energy production factor is higher by 6.25% and exergy‐based life cycle conversion efficiency is higher by 96.39% for N‐CPC‐ETC‐SSU than N‐ETC‐SSU.
Small‐sample properties of robust willingness‐to‐pay estimators
Authors: Lambert Dayton M. 
Published: July 2024

AbstractThe small‐sample properties of robust, binary choice willingness‐to‐pay (WTP) estimators are analyzed. A Monte Carlo simulation compares the bias and mean‐squared error of marginal and expected WTP estimates from probit, zero‐centered (“robit”), logit, and a generalized Tukey lambda distribution called a “pregibit” regression under normal and nonnormal distributional assumptions. Robust binary choice estimators allow for variation in tail thickness (the robit and pregibit) or tail asymmetry (the pregibit). No previous studies have compared the performance of these WTP estimators. The findings will interest researchers who use contingent validation methods to estimate WTP for nonmarket or hypothetical goods.
Prognostic value and potential regulatory relationship of miR‐200c‐5p in colorectal cancer
Authors: Lu Jiying , Jin Zhekang , Jin Xihan , Chen Wenbin 
Published: July 2024

AbstractThis study aimed to investigate the relationship and potential mechanisms of miR‐200c‐5p in colorectal cancer (CRC) progression. Differentially expressed miRNAs were screened using the TCGA database. Subsequently, univariate analysis was performed to identify CRC survival‐related miRNAs. Survival and receiver operator characteristic curves were generated. The target genes of miR‐200c‐5p and the relevant signaling pathways or biological processes were predicted by the miRNet database and enrichment analyses. The miR‐200c‐5p expression was detected using quantitative reverse‐transcription polymerase chain reaction, Cell Counting Kit‐8, Transwell, and cell apoptosis experiments were performed to determine miR‐200c‐5p's impact on CRC cell viability, invasiveness, and apoptosis. Finally, we constructed a CRC mouse model with inhibited miR‐200c‐5p to evaluate its impact on tumors. miR‐200c‐5p was upregulated in CRC, implying a favorable prognosis. Gene set enrichment analysis revealed that miR‐200c‐5p may participate in signaling pathways such as the TGF‐β signaling pathway, RIG‐I‐like receptor signaling pathway, renin‐angiotensin system, and DNA replication. miR‐200c‐5p potentially targeted mRNAs, including KCNE4 and CYP1B1, exhibiting a negative correlation with their expression. Furthermore, these mRNAs may participate in biological processes like the regulation of intracellular transport, cAMP‐dependent protein kinase regulatory activity, ubiquitin protein ligase binding, MHC class II protein complex binding, and regulation of apoptotic signaling pathway. Lastly, miR‐200c‐5p overexpression repressed the viability and invasiveness of CRC cells but promoted apoptosis. The tumor size, weight, and volume were significantly increased by inhibiting miR‐200c‐5p (p < 0.05). miR‐200c‐5p is upregulated in CRC, serving as a promising biomarker for predicting CRC prognosis.
Activation of α7 Nicotinic Acetylcholine Receptor Improves Muscle Endurance by Upregulating Orosomucoid Expression and Glycogen Content in Mice
Authors: Chen Fei , Zhang Zhen , Zhang Huimin , Guo Pengyue , Feng Jiayi , Shen Hui , Liu Xia 
Published: July 2024

ABSTRACTThere are presently no acknowledged therapeutic targets or official drugs for the treatment of muscle fatigue. The alpha7 nicotinic acetylcholine receptor (α7nAChR) is expressed in skeletal muscle, with an unknown role in muscle endurance. Here, we try to explore whether α7nAChR could act as a potential therapeutic target for the treatment of muscle fatigue. Results showed that nicotine and PNU‐282987 (PNU), as nonspecific and specific agonists of α7nAChR, respectively, could both significantly increase C57BL6/J mice treadmill‐running time in a time‐ and dose‐dependent manner. The improvement effect of PNU on running time and ex vivo muscle fatigue index disappeared when α7nAChR deletion. RNA sequencing revealed that the differential mRNAs affected by PNU were enriched in glycolysis/gluconeogenesis signaling pathways. Further studies found that PNU treatment significantly elevates glycogen content and ATP level in the muscle tissues of α7nAChR+/+ mice but not α7nAChR‐/‐ mice. α7nAChR activation specifically increased endogenous glycogen‐targeting protein orosomucoid (ORM) expression both in vivo skeletal muscle tissues and in vitro C2C12 skeletal muscle cells. In ORM1 deficient mice, the positive effects of PNU on running time, glycogen and ATP content, as well as muscle fatigue index, were abolished. Therefore, the activation of α7nAChR could enhance muscle endurance via elevating endogenous anti‐fatigue protein ORM and might act as a promising therapeutic strategy for the treatment of muscle fatigue.
TRPV1‐dependent NKCC1 activation in mouse lens involves integrin and the tubulin cytoskeleton
Authors: Shahidullah Mohammad , Mandal Amritlal , Delamere Nicholas A. 
Published: July 2024

AbstractPreviously we showed hyperosmotic solution caused TRPV1‐dependent NKCC1 activation in the lens by a mechanism that involved ERK1/2 signaling. In various tissues, integrins and the cytoskeletal network play a role in responses to osmotic stress. Here, we examined the association between integrins and TRPV1‐dependent activation of NKCC1 in mouse lens epithelium. Wild‐type (WT) lenses exposed to the integrin agonist leukadherin‐1 (LA‐1) for 10 min displayed a ~33% increase in the bumetanide‐sensitive rate of Rb uptake indicating NKCC activation. Paclitaxel, a microtubule stabilizing agent, abolished the Rb uptake response. In primary cultured lens epithelium LA‐1 caused a robust ERK1/2 activation response that was almost fully suppressed by paclitaxel. The TRPV1 agonist capsaicin caused a similar ERK1/2 activation response. Consistent with an association between integrins and TRPV1, the TRPV1 antagonist A889425 prevented the Rb uptake response to LA‐1 as did the ERK inhibitor U0126. LA‐1 did not increase Rb uptake by lenses from TRPV1 knockout mice. In cells exposed to a hyperosmotic stimulus, both the ERK1/2 activation and Rb uptake responses were prevented by paclitaxel. Taken together, the findings suggest TRPV1 activation is associated with integrins and the tubulin cytoskeleton. This aligned with the observation that LA‐1 elicited a robust cytoplasmic calcium rise in cells from WT lenses but failed to increase calcium in cells from TRPV1 knockout lenses. The results are consistent with the notion that integrin activation by LA‐1, or a hyperosmotic stimulus, causes TRPV1 channel opening and the consequent downstream activation of the ERK1/2 and NKCC1 responses.
Enhancing point‐of‐care ultrasound (POCUS) utilization in primary care: A thyroid POCUS training course for internal medicine residents
Authors: D'Agostino Christina , Block Lauren , Smith Alexander , Weintraub Spencer , Rose Sage , Coletti Daniel J. , Khamis Nehal 
Published: July 2024

AbstractBackgroundPOCUS is valuable in primary care, yet outpatient‐specific point‐of‐care ultrasound (POCUS) curriculum integration into internal medicine (IM) residency is limited. We addressed this gap by developing a thyroid POCUS workshop for IM residents.AimDevelop and implement an educational curriculum to integrate thyroid POCUS into an IM residency program and evaluate the impact on resident knowledge, perceived skills, and attitudes.SettingThe study was conducted in a resident primary care clinic at the Donald and Barbara Zucker School of Medicine at Hofstra/Northwell Residency Program in Internal Medicine at North Shore University Hospital and Long Island Jewish Medical Center.ParticipantsAll 108 IM residents (PGY1‐3) in one program participated in the study during their ambulatory clinic block.Program descriptionResidents participated in a 1‐hour workshop involving a didactic session and two breakout groups: one for hands‐on practice and another for case‐based discussions with image review.Program evaluationResidents completed pre‐ and post‐session surveys assessing knowledge, perceived skills, and attitudes toward thyroid POCUS. These data showed statistically significant increases in all assessed areas.DiscussionIntegrating thyroid POCUS into an IM residency curriculum significantly improved resident knowledge, attitudes, and perceived skills related to these exams. Residents valued this learning experience and expressed intentions to incorporate it into their future practice.
The trajectory of anxiety symptoms during the transition from childhood to young adulthood is predicted by IQ and sex, but not polygenic risk scores
Authors: Ravagnani Salto Ana Beatriz , Salum Giovanni A. , Hoffmann Maurício Scopel , Santoro Marcos L. , Zugman André , Pan Pedro M. , Belangero Sintia I. , Ito Lucas Toshio , Doretto Victoria Fogaça , Croci Marcos S. , Brañas Marcelo J. A. A. , de Giusti Carina , Silva‐Jr Francisco Da , Ribeiro Sahâmia Martins , Miguel Euripedes Constantino , Leckman James F. 
Published: July 2024

AbstractBackgroundUnderstanding the factors that determine distinct courses of anxiety symptoms throughout development will better guide interventions. There are scarce data‐driven longitudinal studies, using multi‐modal predictors, investigating the chronicity of anxiety symptoms from childhood to young adulthood, particularly in a middle‐income country.Methods2033 youths (ages 6–14 years [Mean age = 10.4 ± 1.94) at Baseline] were enrolled in the Brazilian High‐Risk Cohort for Mental Conditions longitudinal study, and assessed at three timepoints, between 2010 and 2019, using the Screen for Child Anxiety Related Disorders. Confirmatory Factor Analysis provided input to Growth Mixture Models to identify the best fitting trajectory model. Multinomial logistic regression analyses tested the effects of intelligence quotient (IQ), environmental factors and polygenic risk scores on internalizing symptomatology within trajectory class membership.ResultsThe best model solution identified three classes: high‐decreasing, moderate/low‐stable and low‐increasing symptoms over time. The high‐decreasing class showed a higher incidence of anxiety symptoms at the second time point (Mean age = 13.8 ± 1.93); while anxiety symptoms were highest in the low‐increasing class at the third timepoint (Mean age = 18.35 ± 2.03). Further, lower IQ predicted membership in the high‐decreasing trajectory class (OR = 0.68, 95% CI [0.55, 0.85]), while higher IQ predicted membership in the low‐increasing trajectory class (OR = 1.95, 95% CI [1.42, 2.67]). Finally, females were more likely than males to be in the low‐increasing trajectory class. Polygenic risk scores were not associated with anxiety trajectory class membership.ConclusionRecognizing that anxiety symptoms follow diverse paths over time will allow for more effective intervention strategies. Specifically, interventions could accommodate children for greater anxiety risk in early childhood (i.e., lower IQ) versus late adolescence (i.e., higher IQ). That said, the emotional needs of girls in late adolescence should be monitored, regardless of their cognitive abilities or high achievements.
Qualitative and Untargeted Volatilome Fingerprinting of Aspergillus sp. and Bulbithecium sp. by HS‐SPME‐GCMS and Functional Interactions
Authors: Mancheary John Prathyash Ushus , Kandula Siva Kumar , Cheekatla Satyanarayana Swamy , Metta Venkata Satya Mahesh Kumar , Peddi Koteswari 
Published: July 2024

ABSTRACTResearch on fungal volatile organic compounds (VOCs) has increased worldwide in the last 10 years, but marine fungal volatilomes remain underexplored. Similarly, the hormone‐signaling pathways, agronomic significance, and biocontrol potential of VOCs in plant‐associated fungi make the area of research extremely promising. In the current investigation, VOCs of the isolates—Aspergillus sp. GSBT S13 and GSBT S14 from marine sediments, and Bulbithecium sp. GSBT E3 from Eucalyptus foliage were extracted using Head Space solid phase microextraction, followed by gas chromatography‐mass spectrometry, identification, statistical analyses, and prediction of functions by KEGG COMPOUND and STITCH 5.0 databases. The significance of this research is fingerprinting VOCs of the isolates from distinct origins, identification of compounds using three libraries (NIST02, NIST14, and W9N11), and using bioinformatic tools to perform functional analysis. The most important findings include the identification of previously unreported compounds in fungi—1‐methoxy naphthalene, diethyl phthalate, pentadecane, pristane, and nonanal; the prediction of the involvement of small molecules in the degradation of aromatic compound pathways and activation, inhibition, binding, and catalysis of metabolites with predicted protein partners. This study has ample opportunity to validate the findings and understand the mechanism or mode of action, the interspecies interactions, and the role of the metabolites in geochemical cycles.