bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proinflammatory mediators alter expression of nuclear factor kappa B–regulating deubiquitinases in sinonasal epithelial cells
Authors: Li Ping , Wang Ying , Turner Justin H. 
Published: April 2015

BackgroundNuclear factor κB (NF‐κB) is a vital transcription factor that is activated by numerous inflammatory stimuli. Its activity is tightly regulated by a family of deubiquitinating enzymes (A20, Cezanne, cylindromatosis [CYLD]) that function in a negative‐feedback loop, a process that prevents chronic and systemic inflammation. This study seeks to characterize the expression and functional role of NF‐κB–regulating deubiquitinases in the sinonasal epithelium.MethodsExpression of A20, Cezanne, and CYLD was assessed in normal sinonasal tissue using immunohistochemistry. Cultured sinonasal epithelial cells (SNECs) were stimulated with proinflammatory cytokines (tumor necrosis factor α [TNF‐α], interleukin 4 [IL]‐4, IL‐13) or lipopolysaccharide (LPS) and changes in NF‐κB activation and deubiquitinase expression were assessed using Western blots and quantitative real‐time polymerase chain reaction (qRT‐PCR), respectively.ResultsNF‐κB was activated in response to LPS and TNF‐α, but not IL‐4 or IL‐13. A20, Cezanne, and CYLD were all expressed in sinonasal tissue, primarily along the apical surface of the epithelium. Proinflammatory mediators primarily affected expression of A20, with upregulation by LPS and TNF‐α and downregulation by IL‐4 and IL‐13.ConclusionThe NF‐κB–regulating deubiquitinases A20, Cezanne, and CYLD are expressed in sinonasal tissue and are differentially induced by proinflammatory cytokines and the microbial antigen, LPS. These results suggest an important role for NF‐κB–regulating deubiquitinases in mucosal immunity and homeostasis.
Genetic Incorporation of Nε‐Formyllysine, a New Histone Post‐translational Modification
Authors: Wang Tianyuan , Zhou Qing , Li Fahui , Yu Yang , Yin Xuebin , Wang Jiangyun 
Published: April 2015

AbstractLysine formylation is a newly discovered post‐translational modification (PTM) in histones and other nuclear proteins; it has a well‐recognized but poorly defined role in chromatin conformation modulation and gene expression. To date, there is no general method to site‐specifically incorporate Nε‐formyllysine at a defined site of a protein. Here we report the highly efficient genetic incorporation of the unnatural amino acid Nε‐formyllysine into proteins produced in Escherichia coli and mammalian cells, by using an orthogonal Nε‐formyllysine tRNAsynthetase/tRNACUA pair. This technique can be applied to study the role of lysine formylation in epigenetic regulation.
The role of children's bone marrow mesenchymal stromal cells in the ex vivo expansion of autologous and allogeneic hematopoietic stem cells
Authors: Pelagiadis Iordanis , Stiakaki Eftichia , Choulaki Christianna , Kalmanti Maria , Dimitriou Helen 
Published: April 2015

AbstractThe recognition of the role of Mesenchymal Stromal Cells (MSC) in hematopoiesis, as part of the bone marrow microenvironment, renewed the interest for cord blood (CB) ex vivo expansion as a source of HSC for transplantation. MSC from children are recognized to have different biological properties compared to the ones from adults. The current study focuses on the evaluation of the effects of children's bone marrow MSC on the ex vivo expansion capacity of both allogeneic cord blood and autologous bone marrow (BM) CD34+ hematopoietic stem cells (HSCs) when used as a cell feeder layer with or without recombinant cytokines. Our results showed that children's bone marrow‐derived MSC expand more primitive populations in co culture with CD34 and that the expansion is further enhanced when the culture is supplemented with growth factors. No additive effect was seen either with the early‐ or late‐acting growth factors' cocktails used. Biological features of CB hematopoietic progenitors seem to make them more suitable than their BM counterparts for ex vivo expansion. Clinical implementation will be facilitated by methodological standardization and guidelines' establishment.
Comparison between two‐ and three‐dimensional quantitative coronary angiography bifurcation analyses for the assessment of bifurcation lesions: A subanalysis of the TRYTON pivotal IDE coronary bifurcation trial
Authors: Muramatsu Takashi , Grundeken Maik J. , Ishibashi Yuki , Nakatani Shimpei , Girasis Chrysafios , Campos Carlos M. , Morel Marie‐Angèle , Jonker Hans , de Winter Robbert J. , Wykrzykowska Joanna J. , García‐García Hector M. , Leon Martin B. , Serruys Patrick W. , Onuma Yoshinobu ,   On behalf of the TRYTON Pivotal IDE Coronary Bifurcation Trial Investigators
Published: April 2015

BackgroundThree‐dimensional (3D) quantitative coronary angiography (QCA) provides more accurate measurements by minimizing inherent limitations of two‐dimensional (2D) QCA. The aim of this study was to compare the measurements between 2D and 3D QCA analyses in bifurcation lesions.Methods and ResultsA total of 114 cases with non‐left main bifurcation lesions in the TRYTON pivotal IDE Coronary Bifurcation Trial (ClinicalTrials.gov: NCT01258972) were analyzed using a validated bifurcation QCA software (CAAS 5.10, Pie Medical Imaging, Maastricht, the Netherlands). All cases were analyzed in matched projections between pre‐ and post‐procedure. The 2D analysis was performed using one of two angiographic images used for 3D reconstruction showing a larger distal bifurcation angle. In the treated segments (stent and balloon), there were no differences in minimal luminal diameter (MLD) between 2D and 3D, while diameter stenosis (DS) was significantly higher in 2D compared to 3D both pre‐procedure and post‐procedure (53.9% for 2D vs. 52.1% for 3D pre‐procedure, P < 0.01; 23.2% for 2D vs. 20.9% for 3D post‐procedure, P = 0.01). In the sub‐segment level analysis, lengths of proximal main branch, distal main branch, and side branch were consistently shorter in 2D compared to 3D both pre‐procedure and post‐procedure. Using 3D QCA, the anatomic location of the smallest MLD or the highest DS was relocated to a different bifurcation sub‐segment in a considerable proportion of the patients compared to when 2D‐QCA was used (kappa values: 0.50 for MLD, 0.55 for DS).ConclusionsOur data showed differences in addressing anatomical severity and location of coronary bifurcation lesions between in vivo 2D and 3D QCA analyses. More studies are needed to investigate potential clinical benefits in using 3D approach over 2D QCA for the assessment of bifurcation lesions. © 2015 Wiley Periodicals, Inc.
Transcatheter balloon dilation for recurrent right ventricular outflow tract obstruction following valve‐sparing repair of tetralogy of Fallot
Authors: Gellis Laura , Banka Puja , Marshall Audrey , Emani Sitaram , Porras Diego 
Published: April 2015

BackgroundValve‐sparing repair in patients with tetralogy of Fallot (TOF) carries the risk of residual or recurrent right ventricular outflow tract (RVOT) obstruction, which is often treated with transcatheter balloon dilation (BD). The outcomes and associated complications of BD of the RVOT in this scenario remain unknown.MethodsRetrospective review of the records of the Department of Cardiology at Boston Children's Hospital from 2000 to 2013 was performed.Results34 patients had initial valve‐sparing repair of tetralogy of Fallot followed by BD of the RVOT during the study period. Following BD, the RVOT gradient decreased from a median of 43 mm Hg (range 13 to 79 mm Hg) to 28 mm Hg (range 0 to 73 mm Hg) (P < 0.001). Freedom from reintervention was 64% at 1 year and 46% at 3 years. Trivial to mild PR pre‐BD was present in 56% (n = 19) of patients and decreased to 37% (n = 11) post‐BD. Exclusively valvar obstruction was associated with a longer freedom from reintervention (P = 0.05), while a ratio of RV pressure to aortic systolic pressure pre‐BD of >1 and a final RVOT gradient of ≥40 post‐BD were associated with shorter freedom from reintervention (P < 0.001).ConclusionBD in patients with recurrent RVOT obstruction following valve‐sparing repair of TOF acutely reduces the RVOT gradient, but commonly results in increased PR and is associated with a high reintervention rate. Patients with stenosis solely at the level of the valve had a better response to this type of intervention. © 2015 Wiley Periodicals, Inc.
Short‐ and long‐term outcomes in diabetes patients undergoing percutaneous coronary intervention with bivalirudin compared with heparin and glycoprotein IIb/IIIA inhibitors: A meta‐analysis of randomized trials
Authors: Nairooz Ramez , Sardar Partha , Amin Hossam , Chatterjee Saurav , Helmy Tarek , Naidu Srihari S. 
Published: April 2015

BackgroundDiabetes patients undergoing percutaneous coronary intervention (PCI) have more complications than nondiabetes patients, including increased long‐term mortality. Use of bivalirudin versus heparin and glycoprotein IIb/IIIa inhibitors (GPI) in diabetes patients undergoing PCI and its effect on long‐term mortality were evaluated in few randomized trials, but with conflicting results.MethodsWe searched the literature for randomized controlled trials that compared heparin and GPI therapy with bivalirudin in diabetes patients undergoing PCI. The incidence of major adverse cardiovascular events (MACE), death from any cause, myocardial infarction (MI), urgent revascularization, major and minor bleeding (at 30 days), as well as all‐cause mortality at 1 year were included, and meta‐analysis was performed.ResultsA total of 5,137 patients with diabetes were included in four randomized trials. At 30 days, bivalirudin, compared with heparin and GPI, caused less major bleeding (odds ratio (OR), 0.68; 95% confidence interval (CI), 0.52–0.89; P = 0.005) and less minor bleeding (OR, 0.48; 95% CI, 0.41–0.57; P < 0.00001) and similar rates of MACE (OR, 0.87; 95% CI, 0.70–1.08; P = 0.21), MI (OR, 0.87; 95% CI, 0.68–1.10; P = 0.25), and urgent revascularization (OR, 1.12; 95% CI, 0.76–1.65; P = 0.57). Death from any cause at 30 day was numerically lower with bivalirudin use but not statistically significant (OR, 0.72; 95% CI, 0.46–1.13; P = 0.15). Mortality at 1 year was significantly lower in diabetes patients treated with bivalirudin compared with heparin and GPI (OR, 0.72; 95% CI, 0.52–0.99; P = 0.04). A secondary analysis suggests that the major bleeding benefit with bivalirudin may be driven by mandated use of GPI in heparin arm.ConclusionAmong patients with diabetes undergoing PCI, bivalirudin caused less major and minor bleeding compared with heparin and GPI, with similar rates of MACE, death, MI, and urgent revascularization at 30 days, but significantly lower mortality rates at 1 year. © 2015 Wiley Periodicals, Inc.
Isomer Treatment by Additive Application in the Liquid Stage as Known from Industrial Crystallization
Authors: Kreißig Franziska , Schäfer Christian , Verdurand Elodie , Ulrich Joachim 
Published: April 2015

AbstractThis study verified the usefulness of additives for the stabilization of dissolved material in the liquid stage as in the case of crystallization. Additives were introduced to prevent dissolved crystals from isomerization and degradation. The case study here is astaxanthin, a carotenoid occurring in different isomers. The molecule structure is susceptible to a plethora of substances and factors which cause transformation and degradation of the isomers. The aim was to examine whether foreign materials (additives) would induce a change of the kinetics of a system consisting of crystalline all‐trans astaxanthin dissolved in chloroform. The results show that K2CO3 and CH3COOK increase the stability of all‐trans astaxanthin in solution.
Effects of trans‐10, cis‐12‐conjugated linoleic acid on mice are influenced by the dietary fat content and the degree of murine obesity
Authors: Li Shi‐Li , Ma Shu‐Yu , Xu Bin‐Rui , Fan Zhi‐Ying , Li Mei‐Juan , Cao Wen‐Guang , Gou Ke‐Mian 
Published: April 2015

Trans‐10, cis‐12‐conjugated linoleic acid (t10c12‐CLA) has been reported to induce body fat loss with the side effects of liver steatosis and the development of diabetes. To investigate whether the dietary fat content may influence the response to t10c12‐CLA in animals, t10c12‐producing recombinant food‐grade Lactococcus lactis transformed with the pai isomerase gene was used to feed lean, obesity‐induced, or obese mice. Compared with the non‐CLA producing recombinant, the significant (p < 0.05) changes in the lean mice were the loss of body weight and abdominal adipose tissue (p < 0.05), reduced ratios of total cholesterol/HDL, and LDL/HDL (p < 0.05), and an early hepatic steatosis. In obesity‐induced mice, the body weight and abdominal adipose tissue were unexpectedly increased (p < 0.05) with an elevated TG/HDL ratio (p < 0.05). In obese mice, only the ratios of LDL/HDL and total cholesterol/HDL were significantly (p < 0.05) reduced. In addition, within three groups, certain changes in fatty acid composition also occurred in various organs and tissues. These results suggest that the dietary fat level and host obesity can alter the response to t10c12‐CLA in animals.Practical Applications: Trans‐10, cis‐12‐CLA has been commercialized as a functional food or nutraceutical because of its multiple effects on body health; for example it may reduce body fat. However, a few studies have also found that t10c12‐CLA had no effect on losing fat and has side effects such as non‐alcoholic liver steatosis. This study provides deeper insights, revealing that the health effect of t10c12‐CLA is also influenced by the dietary fat content or host obesity degree. The results of this study may help to explain the inconsistencies in animal experiments and in human clinical studies on t10c12‐CLA.Differences (*p < 0.05) in body weight gain (%) in lean, obesity‐induced, or obese mice that received t10c12‐producing (▴) or non‐CLA producing (●) L. lactis.
Would you post that picture and let your dad see it? Culture, honor, and Facebook
Authors: Günsoy Ceren , Cross Susan E. , Sarıbay Adİl , Olcaysoy Ökten Irmak , Kurutaş Meltem 
Published: April 2015

AbstractHonor means “high respect; esteem,” but it has different associations for different cultures. In honor cultures (Turkey), esteem depends on one's own perception of self‐worth and on other people's opinions. In those cultures, honor is easily lost and difficult to regain. In dignity cultures (northern America), esteem mainly depends on the individual and cannot be taken away by others. One way to lose honor in Turkey is through behaviors that may be seen as “potentially improper.” Thus, we expected that posting pictures of such behaviors on Facebook (e.g., at a party; with one's boyfriend/girlfriend) and letting others see them would be less likely in Turkey than in the northern United States. Moreover, we investigated whether honor endorsement was the reason for this difference. We examined participants' posting intentions and actual Facebook behaviors. As expected, Turkish participants were less willing to post and let others (especially their relatives) see their potentially improper pictures compared with northern Americans. Moreover, honor endorsement negatively predicted the willingness to post such pictures only in Turkey, especially for women. This suggests that in honor cultures, the concern for losing honor could be the underlying reason for avoiding social media postings that could be potentially perceived as improper. Copyright © 2015 John Wiley & Sons, Ltd.
Potentiometric Biosensor System Based on Recombinant Urease and Creatinine Deiminase for Urea and Creatinine Determination in Blood Dialysate and Serum
Authors: Marchenko S. V. , Kucherenko I. S. , Soldatkin O. O. , Soldatkin A. P. 
Published: April 2015

AbstractA biosensor system for simultaneous determination of creatinine and urea in blood serum and dialysate samples was developed. It consisted of creatinine and urea biosensors based on a potentiometric transducers with two identical pH‐sensitive field‐effect transistors. In creatinine biosensor, creatinine deiminase immobilized via photopolymerization in PVA/SbQ polymer on one transistor served as a biorecognition element, while bovine serum albumin in PVA/SbQ polymer placed on the second transistor was used for reference. The urea biosensor was created in the same way but recombinant urease was used instead of creatinine deiminase. The linear ranges of creatinine and urea measurement were 0.02–2 mM and 0.5–15 mM, correspondingly, which allowed simultaneous determination of the metabolites. Response time of the biosensor system was 2–3 min; RSD of responses did not exceeded 5 %. The biosensors demonstrated absence of non‐selective response towards components of blood dialysate and serum. Urea and creatinine concentrations were determined in 20 samples of blood dialysate and serum. The results correlated well with traditional methods of analysis. Creatinine and urea biosensors were stable during five months of storage (during this time the responses decreased by about 10 %). The proposed biosensor system can be effectively used for analysis of serum samples and for hemodialysis control.
Partial‐Load Analysis of a Temperature‐Controlled Solid‐Oxide Fuel Cell–Gas Turbine (SOFC–GT) Hybrid Power Plant
Authors: Zhang Yu , Zhao Jiyun , Wang Peng , Xiong Binyu , Xian Liang , Badrinarayanan Rajagopalan 
Published: April 2015

AbstractDue to high conversion efficiency and low environmental impact, solid‐oxide fuel cells coupled with a gas turbine (SOFC–GT) have received much attention. A proper stack temperature control strategy ensures safety and enhances performance. A heat management subsystem capable of adjusting the stack temperature is critical for the hybrid plant. Most present SOFC–GT plants deliver the full volume of the GT’s exhaust to heat up the stack. Stack cooling is realized by blowing extra air, which consumes more parasitic energy and impacts the plant’s conversion efficiency. In this study, a heat management subsystem has been added into the SOFC–GT plant. The stack average temperature can be adjusted by controlling the volume of the GT’s exhaust and additional fuel. In this way, the parasitic energy owing to the air compressor is reduced. To investigate the proposed plant’s performance, a corresponding model has been established. Simulation results of the specific operating point show that the heat management subsystem can maintain the stack average temperature at its proper value. Furthermore, based on the different air/additional fuel supply patterns, the partial‐load performance of the proposed plant has been compared, and an optimal partial‐load strategy is proposed. The proposed SOFC–GT plant shows a wide output range (370.0–1540.7 kW) with remarkable efficiency (56.8 %–75.0 %).
Determination of optimum oven cooking procedures for lean beef products
Authors: Rodas‐González Argenis , Larsen Ivy L. , Uttaro Bethany , Juárez Manuel , Parslow Joyce , Aalhus Jennifer L. 
Published: April 2015

AbstractIn order to determine optimum oven cooking procedures for lean beef, the effects of searing at 232 or 260°C for 0, 10, 20 or 30 min, and roasting at 160 or 135°C on semimembranosus (SM) and longissimus lumborum (LL) muscles were evaluated. In addition, the optimum determined cooking method (oven‐seared for 10 min at 232°C and roasted at 135°C) was applied to SM roasts varying in weight from 0.5 to 2.5 kg. Mainly, SM muscles seared for 0 or 10 min at 232°C followed by roast at 135°C had lower cooking loss, higher external browning color, more uniform internal color, and were more tender and flavorful (P < 0.05). Roast weights ≥1 kg had lesser cooking loss, more uniform internal color and tender compared to 0.5 kg (P < 0.05). Consequently, roasting at low temperature without searing is the recommended oven cooking procedure; with best response from muscle roast weight ≥1 kg.
Plant architecture, plasticity, and adaptation strategies of two oat genotypes under different competition intensities
Authors: Li Pu‐Fang , Ma Bao‐Luo , Yan Weikai , Cheng Zheng‐Guo , Li Feng‐Min , Xiong You‐Cai 
Published: April 2015

AbstractBACKGROUNDThe hypothesis that positive and negative interactions account for adaptive strategies was tested in a controlled study with two oat (Avena sativa) genotypes: ‘Manotick’ with erect leaves and ‘Oa1316‐1’ with prostrate leaves. An increasing competition pattern was designed by varying the number of seeds planted in each container and the space between containers, thus creating different planting density regimes (i.e. alternative and solid treatments).RESULTSTotal biomass of individual plants tended to decrease exponentially with increasing density in both genotypes. Under high density stress, Manotick allocated more biomass to the roots and produced 50% more tillers, leading to more non‐productive tillers and lower harvest index in the alternative than in the solid treatment. In contrast, Oa1316‐1 allocated more biomass to panicles and stems, and less to the roots, with fewer tillers.CONCLUSIONSWith increasing density and strengthening intraspecific competition, Manotick reduced aboveground biomass allocation, leading to lower yield, while Oa1316‐1 decreased allocation to the roots, but increased allocation to the panicles under an increasingly competitive environment. These adjustments were mechanically derived from negative and positive interactions, ensuring greater yield in the prostrate type. Our findings provided a novel rationale for a planting strategy based on plant type selections. © 2015 Her Majesty the Queen in Right of Canada. Journal of the Science of Food and Agriculture © 2015 Society of Chemical Industry
Simultaneous detection of bacitracin and polymyxin B in livestock products using liquid chromatography with tandem mass spectrometry
Authors: Zhang Dan , Park Jin‐A , Kim Dong‐Soon , Kim Na‐Hyun , Kim Seong‐Kwan , Cho Kyeong‐Su , Jeong Dana , Shim Jae‐Han , Abd El‐Aty A. M. , Shin Ho‐Chul 
Published: April 2015

With the overarching aim to develop a simple and reliable method for the quantitative analysis of polypeptide antibiotics in various livestock products, the content of bacitracin, and polymyxin B in pork, beef, chicken, milk, and eggs was analyzed using colistin sulfate as an internal standard. The extracted samples were eluted via solid‐phase extraction using 2% formic acid in acetonitrile/methanol (1:1, v/v). The two polypeptides were identified and quantified based on the intensities of mass fragments from the respective triply charged precursor ions (bacitracin: 474.97 amu and polymyxin B: 402 amu) at the defined retention time windows using liquid chromatography with electrospray ionization tandem mass spectrometry in time‐scheduled multiple reaction monitoring mode. The calibration curves showed good linearity over the concentration range 50–2500 ng/mL with determination coefficients ≥ 0.991. The mean recoveries were in the range 80.3–88.8% with relative standard deviations <13% for all samples. The limits of quantitation ranged from 30–250 ng/g. The developed method was applied to market samples, but the target analytes were not detected in any of the samples. The developed method is reliable for the simultaneous detection of bacitracin and polymyxin B in pork, beef, chicken, milk, and eggs.