bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depression in patients with idiopathic pulmonary fibrosis
Authors: Akhtar Akhtar A. , Ali Mohammad A. , Smith Robin P. 
Published: July 2013

Depression carries enormous global morbidity and is 1.5–7 times likelier to occur in individuals with chronic illness than in the general population. Idiopathic pulmonary fibrosis (IPF) has a rising incidence with a severe impact on quality of life. An indication of the prevalence of depression in this group is therefore of paramount interest. A prospective study was performed. A total of 118 participants with IPF who attended the interstitial lung disease clinic in Ninewells Hospitals, Dundee, Scotland, from May 2010 to September 2011 were recruited. Informed consent was obtained. The male to female ratio was 60:58. The Wakefield Self-assessment of Depression Inventory was used (scores ≥15 denote a depressed state). Pulmonary function tests were measured to correlate disease severity with depression scores. Of them, 58 patients had significant depressive symptoms scoring ≥15; only nine were taking antidepressant medication. The mean depression score of female participants was 15.0 ± 0.77 (SD 5.9), compared with a mean male score of 13.1 ± 0.99 (SD 7.5). Disease severity, age, duration since diagnosis and number of co-morbidities were not significantly correlated with depression. The study population had a high prevalence of depressive symptoms. Medical therapy for pulmonary fibrosis is limited and therefore palliation of symptoms and pulmonary rehabilitation form the main strategy for management. Depression should be actively screened in patients with IPF.
Frequency and management of respiratory incidents in invasive home ventilation
Authors: Stieglitz Sven , George Sandhya , Priegnitz Christina , Hagmeyer Lars , Randerath Winfried 
Published: July 2013

There has been a rise in the number of patients requiring long-term ventilation, both in the in-hospital and the out-of-hospital setting. Despite this, little is known about the subsequent clinical course of these patients following hospital discharge. The purpose of this study was to determine the frequency and management of respiratory incidents in patients with invasive out-of-hospital ventilation living in a nursing home allied to a weaning centre. We evaluated retrospectively the protocols that are used to monitor the patients over a period of 2 months. The average time from hospital discharge was 386 ± 330 days. Of the total 17 patients, 9 (53%) patients remained free from any respiratory incidents, while the remaining 8 (47%) patients were responsible for a total of 95 respiratory incidents. Patients that suffered respiratory incidents had been ventilated at home for an average of 194 days, while the others were receiving out-of-hospital ventilation for an average of 557 days. Desaturation (17), dyspnoea (17) and reduced general condition (10) were the most common respiratory incidents. Also, the use of an Ambu bag (bag valve mask; 17), request for a pneumologist review (12) and replacement of the tracheal cannula (7) were the most common interventions. Respiratory incidents are common in invasive home mechanical ventilation, and so home mechanical ventilation needed to be organized safely. Being allied to a weaning centre helps to organize invasive home mechanical ventilation in a safe manner over the long-term ventilation.
Kindlin-2 regulates hemostasis by controlling endothelial cell–surface expression of ADP/AMP catabolic enzymes via a clathrin-dependent mechanism
Authors: Pluskota Elzbieta , Ma Yi , Bledzka Kamila M. , Bialkowska Katarzyna , Soloviev Dmitry A. , Szpak Dorota , Podrez Eugene A. , Fox Paul L. , Hazen Stanley L. , Dowling James J. , Ma Yan-Qing , Plow Edward F. 
Published: July 2013

Key Points
Kindlin-2 regulates hemostasis in vivo by limiting CD39 and CD73 expression on the surface of endothelial cells. Kindlin-2 interacts directly with CHC and controls clathrin-dependent CD39 and CD73 endocytosis/recycling in endothelial cells.
Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
Authors: Coppola Valentina , Coppola Mariangela , Rocco Mariapina , Digilio Maria Cristina , D’Ambrosio Chiara , Renzone Giovanni , Martinelli Rosanna , Scaloni Andrea , Pennacchio Francesco , Rao Rosa , Corrado Giandomenico 
Published: July 2013

Abstract

Background
Aphids are among the most destructive pests in temperate climates, causing significant damage on several crops including tomato. We carried out a transcriptomic and proteomic study to get insights into the molecular mechanisms and dynamics of the tomato response to the Macrosyphum euphorbiae aphid.

Results
The time course analysis of aphid infestation indicated a complex, dynamic pattern of gene expression. Several biological functions were affected and genes related to the stress and defence response were the most represented. The Gene Ontology categories of the differentially expressed genes (899) and identified proteins (57) indicated that the tomato response is characterized by an increased oxidative stress accompanied by the production of proteins involved in the detoxification of oxygen radicals. Aphids elicit a defense reaction based on the cross-communication of different hormone-related signaling pathways such as those related to the salicylic acid (SA), jasmonic acid (JA), ethylene and brassinosteroids. Among them, the SA-signaling pathway and stress-responsive SA-dependent genes play a dominant role. Furthermore, tomato response is characterized by a reduced accumulation of photosynthetic proteins and a modification of the expression of various cell wall related genes.

Conclusions
Our work allowed a more comprehensive understanding of the signaling events and the defense dynamics of the tomato response to aphids in a compatible interaction and, based on experimental data, a model of the tomato–aphid molecular interaction was proposed. Considering the rapid advancement of tomato genomics, this information will be important for the development of new protection strategies.
Ferroelectric BaTiO3/SrTiO3 multilayered thin films for room-temperature tunable microwave elements
Authors: Liu Ming , Ma Chunrui , Collins Gregory , Liu Jian , Chen Chonglin , Alemayehu Andy D , Subramanyam Guru , Ding Ying , Chen Jianghua , Dai Chao , Lin Yuan , Cole Melanie W 
Published: July 2013

Abstract
Ferroelectric BaTiO3/SrTiO3 with optimized c-axis-oriented multilayered thin films were epitaxially fabricated on (001) MgO substrates. The microstructural studies indicate that the in-plane interface relationships between the films as well as the substrate are determined to be (001)SrTiO3//(001)BaTiO3//(001)MgO and [100]SrTiO3//[100]BaTiO3//[100]MgO. The microwave (5 to 18 GHz) dielectric measurements reveal that the multilayered thin films have excellent dielectric properties with large dielectric constant, low dielectric loss, and high dielectric tunability, which suggests that the as-grown ferroelectric multilayered thin films can be developed for room-temperature tunable microwave elements and related device applications.
Predictive Toxicology of cobalt ferrite nanoparticles: comparative in-vitro study of different cellular models using methods of knowledge discovery from data
Authors: Horev-Azaria Limor , Baldi Giovanni , Beno Delila , Bonacchi Daniel , Golla-Schindler Ute , Kirkpatrick James C , Kolle Susanne , Landsiedel Robert , Maimon Oded , Marche Patrice N , Ponti Jessica , Romano Roni , Rossi François , Sommer Dieter , Uboldi Chiara , Unger Ronald E , Villiers Christian , Korenstein Rafi 
Published: July 2013

Abstract

Background
Cobalt-ferrite nanoparticles (Co-Fe NPs) are attractive for nanotechnology-based therapies. Thus, exploring their effect on viability of seven different cell lines representing different organs of the human body is highly important.

Methods
The toxicological effects of Co-Fe NPs were studied by in-vitro exposure of A549 and NCIH441 cell-lines (lung), precision-cut lung slices from rat, HepG2 cell-line (liver), MDCK cell-line (kidney), Caco-2 TC7 cell-line (intestine), TK6 (lymphoblasts) and primary mouse dendritic-cells. Toxicity was examined following exposure to Co-Fe NPs in the concentration range of 0.05 -1.2 mM for 24 and 72 h, using Alamar blue, MTT and neutral red assays. Changes in oxidative stress were determined by a dichlorodihydrofluorescein diacetate based assay. Data analysis and predictive modeling of the obtained data sets were executed by employing methods of Knowledge Discovery from Data with emphasis on a decision tree model (J48).

Results
Different dose–response curves of cell viability were obtained for each of the seven cell lines upon exposure to Co-Fe NPs. Increase of oxidative stress was induced by Co-Fe NPs and found to be dependent on the cell type. A high linear correlation (R2=0.97) was found between the toxicity of Co-Fe NPs and the extent of ROS generation following their exposure to Co-Fe NPs. The algorithm we applied to model the observed toxicity belongs to a type of supervised classifier. The decision tree model yielded the following order with decrease of the ranking parameter: NP concentrations (as the most influencing parameter), cell type (possessing the following hierarchy of cell sensitivity towards viability decrease: TK6 > Lung slices > NCIH441 > Caco-2 = MDCK > A549 > HepG2 = Dendritic) and time of exposure, where the highest-ranking parameter (NP concentration) provides the highest information gain with respect to toxicity. The validity of the chosen decision tree model J48 was established by yielding a higher accuracy than that of the well-known “naive bayes” classifier.

Conclusions
The observed correlation between the oxidative stress, caused by the presence of the Co-Fe NPs, with the hierarchy of sensitivity of the different cell types towards toxicity, suggests that oxidative stress is one possible mechanism for the toxicity of Co-Fe NPs.
Inhibition of Endogenous NGF Degradation Induces Mechanical Allodynia and Thermal Hyperalgesia in Rats
Authors: Osikowicz Maria , Longo Geraldine , Allard Simon , Cuello A Claudio , Ribeiro-da-Silva Alfredo 
Published: July 2013

Background We have previously shown a sprouting of sympathetic fibers into the upper dermis of the skin following subcutaneous injection of complete Freund's adjuvant (CFA) into the hindpaw. This sprouting correlated with an increase in pain-related sensitivity. We hypothesized that this sprouting and pain-related behavior were caused by an increase in nerve growth factor (NGF) levels. In this study, we investigated whether the inhibition of mature NGF degradation, using a matrix metalloproteinase 2 and 9 (MMP-2/9) inhibitor, was sufficient to reproduce a similar phenotype. Results Behavioral tests performed on male Sprague-Dawley rats at 1, 3, 7 and 14 days after intra-plantar MMP-2 /9 inhibitor administration demonstrated that acute and chronic injections of the MMP-2/9 inhibitor induced sensitization, in a dose dependent manner, to mechanical, hot and cold stimuli as measured by von Frey filaments, Hargreaves and acetone tests, respectively. Moreover, the protein levels of mature NGF (mNGF) were increased, whereas the levels and enzymatic activity of matrix metalloproteinase 9 were reduced in the glabrous skin of the hind paw. MMP-2/9 inhibition also led to a robust sprouting of sympathetic fibers into the upper dermis but there were no changes in the density of peptidergic nociceptive afferents. Conclusions These findings indicate that localized MMP-2/9 inhibition provokes a pattern of sensitization and fiber sprouting comparable to that previously obtained following CFA injection. Accordingly, the modulation of endogenous NGF levels should be considered as a potential therapeutic target for the management of inflammatory pain associated with arthritis.
Reduction of needlestick injuries in healthcare personnel at a university hospital using safety devices
Authors: Hoffmann Cornelia , Buchholz Lutz , Schnitzler Paul 
Published: July 2013

Abstract

Background
Healthcare personnel (HCP) is exposed to bloodborne pathogens through occupational risk factors. The objective of this study was to compare the incidence of needlestick injuries (NSIs) before and after the introduction of safety devices in all departments of our hospital.

Methods
Data was extracted from mandatory needlestick report forms of the hospital’s Occupational Health Service. Serological results of patients and healthcare personnel (HCP) were reviewed in the laboratory information system.

Results
In 2007, the year before the introduction of safety devices, 448 needlestick injuries were self-reported, corresponding to an annual rate of 69.0 NSIs per 1 000 full-time HCP. The highest incidence was observed among medical staff in the surgery department and internal medicine with 152 (33.9%) and 79 (17.6%) NSIs, respectively. Of all occupational groups, nurses (36.2%) had the highest risk to sustain NSIs. In 2008 safety devices were introduced across the hospital, e.g. peripheral venous catheter, hypodermic needle and stapling system for wound sealing providing active or passive protection. In 2009, the year after introduction of safety devices, only 350 NSIs were reported, the annual rate of NSIs decreased to 52.4 per 1 000 full-time HCP. Thus an overall reduction of 21.9% for NSIs was achieved when safer devices were applied. The number of NSIs was reduced by even 50% for blood withdrawal, for use of peripheral venous catheters and application of hypodermic needles.

Conclusion
The application of safety devices led to a reduction of NSIs and significantly reduces the risk of bloodborne infections.
Evidence of a new metabolic capacity in an emerging diarrheal pathogen: lessons from the draft genomes of Vibrio fluvialis strains PG41 and I21563
Authors: Khatri Indu , Mahajan Sakshi , Dureja Chetna , Subramanian Srikrishna , Raychaudhuri Saumya 
Published: July 2013

Abstract

Background

Vibrio fluvialis is an emerging diarrheal pathogen for which no genome is currently available. In this work, draft genomes of two closely related clinical strains PG41 and I21563 have been explored.

Results

V. fluvialis strains PG41 and I21563 were sequenced on the Illumina HiSeq 1000 platform to obtain draft genomes of 5.3 Mbp and 4.4 Mbp respectively. Our genome data reveal the presence of genes involved in ethanolamine utilization, which is further experimentally confirmed by growth analysis.

Conclusions
Combined in silico and growth analysis establish a new metabolic capacity of V. fluvialis to harvest energy from ethanolamine.
Mesenchymal stem cell transformation and sarcoma genesis
Authors: Xiao Wei , Mohseny Alexander B , Hogendoorn Pancras C W , Cleton-Jansen Anne-Marie 
Published: July 2013

AbstractMSCs are hypothesized to potentially give rise to sarcomas after transformation and therefore serve as a good model to study sarcomagenesis. Both spontaneous and induced transformation of MSCs have been reported, however, spontaneous transformation has only been convincingly shown in mouse MSCs while induced transformation has been demonstrated in both mouse and human MSCs. Transformed MSCs of both species can give rise to pleomorphic sarcomas after transplantation into mice, indicating the potential MSC origin of so-called non-translocation induced sarcomas. Comparison of expression profiles and differentiation capacities between MSCs and sarcoma cells further supports this. Deregulation of P53- Retinoblastoma-, PI3K-AKT-and MAPK pathways has been implicated in transformation of MSCs. MSCs have also been indicated as cell of origin in several types of chromosomal translocation associated sarcomas. In mouse models the generated sarcoma type depends on amongst others the tissue origin of the MSCs, the targeted pathways and genes and the differentiation commitment status of MSCs. While some insights are glowing, it is clear that more studies are needed to thoroughly understand the molecular mechanism of sarcomagenesis from MSCs and mechanisms determining the sarcoma type, which will potentially give directions for targeted therapies.
Inhibition of VEGF: a novel mechanism to control angiogenesis by Withania somnifera’s key metabolite Withaferin A
Authors: Saha Sanjib , Islam Md Khirul , Shilpi Jamil A , Hasan Shihab 
Published: July 2013

Abstract
Purpose
Angiogenesis, or new blood vessel formation from existing one, plays both beneficial and detrimental roles in living organisms in different aspects. Vascular endothelial growth factor (VEGF), a signal protein, well established as key regulator of vasculogenesis and angiogenesis. VEGF ensures oxygen supply to the tissues when blood supply is not adequate, or tissue environment is in hypoxic condition. Limited expression of VEGF is necessary, but if it is over expressed, then it can lead to serious disease like cancer. Cancers that have ability to express VEGF are more efficient to grow and metastasize because solid cancers cannot grow larger than a limited size without adequate blood and oxygen supply. Anti-VEGF drugs are already available in the market to control angiogenesis, but they are often associated with severe side-effects like fetal bleeding and proteinuria in the large number of patients. To avoid such side-effects, new insight is required to find potential compounds as anti-VEGF from natural sources. In the present investigation, molecular docking studies were carried out to find the potentiality of Withaferin A, a key metabolite of Withania somnifera, as an inhibitor of VEGF.

Methods
Molecular Docking studies were performed in DockingServer and SwissDock. Bevacizumab, a commercial anti-VEGF drug, was used as reference to compare the activity of Withaferin A. X-ray crystallographic structure of VEGF, was retrieved from Protein Data Bank (PDB), and used as drug target protein. Structure of Withaferin A and Bevacizumab was obtained from PubChem and ZINC databases. Molecular visualization was performed using UCSF Chimera.

Results
Withaferin A showed favorable binding with VEGF with low binding energy in comparison to Bevacizumab. Molecular Docking studies also revealed potential protein-ligand interactions for both Withaferin A and Bevacizumab.

Conclusions
Conclusively our results strongly suggest that Withaferin A is a potent anti-VEGF agent as ascertained by its potential interaction with VEGF. This scientific hypothesis might provide a better insight to control angiogenesis as well as to control solid cancer growth and metastasis.
Fabrication of Cellulose-based Microparticles at Gas–Liquid Interfaces in a Microchannel
Authors: Lee Na Rea , Kim Sung Tae , Ko Yeongun , Kim Nam Hee , Chang Suk Tai 
Published: July 2013

Abstract
Cellulose-based microparticles are produced by changing the pH value adjacent to the interfaces between CO2 gas bubbles and an aqueous solution containing the pH-sensitive polymer hydroxypropyl methylcellulose phthalate (HPMCP) in a simple microfluidic system. This method is inexpensive, environmentally friendly, and represents a single-step approach for synthesis of cellulose-based microparticles with a narrow size distribution.
“Don’t forget the migrants”: exploring preparedness and response strategies to combat the potential spread of MERS-CoV virus through migrant workers in Sri Lanka
Authors: Wickramage Kolitha , Peiris Sharika , Agampodi Suneth B 
Published: July 2013

From September 2012 to July 2013, 81 laboratory-confirmed cases of infection with Middle East respiratory syndrome coronavirus (MERS-CoV), including 45 deaths (a case fatality ratio of 55%) have been reported from eight countries. Human-to-human transmission is now confirmed showing potential for another pandemic of zoonotic disease, with an extremely high mortality rate. Effective surveillance strategies are required in countries with a high influx of migrants from the Middle East to mitigate the probable importation of MERS-CoV. We discuss here the risk of MERS-CoV in major labor sending countries and list the probable strategies for control and prevention of MERS-CoV using Sri Lanka as an example. It is conservatively estimated that 10% of Sri Lanka’s population work as international labor migrants (1.8 to 2 million workers), with 93% residing in the Middle East. An average of 720 workers depart each day, with the majority of these workers (71%) departing to the Kingdom of Saudi Arabia (the country with 81.5% of total MERS-CoV cases). We also describe other inbound migration categories such as tourists and resident visa holders relevant to the context of preparedness and planning. The importance of partnerships between public health authorities at national and regional levels with labor migration networks to establish institutional and/or policy mechanisms are highlighted for ensuring effective preparedness and response planning. Strategies that can be taken by public health authorities working in both labor sending and labor receiving counties are also described. The strategies described here may be useful for other labor sending country contexts in Asia with a high frequency and volume of migrant workers to and from the Gulf region.
Wstęp
Authors: Świątek Adam 
Published: July 2013

Tam, gdzie mapa kraju staje się już bardzo południowa, płowa od słońca, pociemniała i spalona od pogód lata, jak gruszka dojrzała – tam leży ona, jak kot w słońcu – ta wybrana kraina, ta prowincja osobliwa, to miasto jedyne na świecie. Daremnie mówić o tym profanom! Daremnie tłumaczyć, że tym długim falistym językiem ziemi, którym dyszy ten kraj w skwarze lata, tym kanikularnym przylądkiem ku Południowi, tą odnogą wsuniętą samotnie między smagłe węgierskie winnice – oddziela się ten partykularz od zespołu krainy i idzie samopas, w pojedynkę, nie wypróbowaną drogą, próbuje na własną rękę być światem. Miasto to i kraina zamknęły się w samowystarczalny mikrokosmos, zainstalowały się na własne ryzyko na samymbrzegu wieczności.
Bruno Schulz, Republika marzeń
Orientacja austro-polska wobec kwestii ukraińskiej w czasie I wojny światowej (do Aktu 5 listopada 1916 r.)
Authors: Drozdowski Mateusz 
Published: July 2013

Матеуш Дроздовський, Австро-угорська орієнтація по відношенню до українського питання підчас Першої світової війни (до Акту 5 листопада 1916 р.)
Напередодні та під час Першої світової війни одночасно сформувався ряд ідей націлених на відновлення польської держави і розвиток українського національного руху. Українські угрупування, що діяли у Галичині, закликали до створення в межах Габсбурзької монархії державної автономної. Польські ж політичні орієнтації у перший період Першої світової війни відстоювали створення польської автономії під протекторатом Габсбургів. Носієм цієї певною мірою австоро-польської ідеї було загальнопольське міжпартійне об’єднання створене в серпні 1914 р. передусім з метою формування польських легіонів під назвою Головний національний комітет. Через те, що ГНЦ проводило масштабні пропагандистські та публіцистичні дії, йому необхідно було зайняти позицію по відношенню до постулатів українських сил. Основна мета цієї статті є розглянути видання ГНЦ: часописи, брошури та статті під кутом оцінки та розуміння українського питання їх авторами. Це дозволить відповісти на питання про відносини поміж активістами з обох угрупувань та про місце українського питання у планах ГНЦ. Оцінка цього періоду у діяльності самої організації та політичної ситуації у монархії є важлива оскільки згодом, у 1918 р. невирішені ідеологічні суперечності поміж українцями та поляками переродились у громадянську війну. Період, що обговорюється у статті більшість цих питань про майбутнє поляків і українців залишались ще відкритими. Текст спирається на документах ГНЦ, доступних у Національному архіві міста Кракові.