bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cavin‐1/PTRF alters prostate cancer cell‐derived extracellular vesicle content and internalization to attenuate extracellular vesicle‐mediated osteoclastogenesis and osteoblast proliferation
Authors: Inder Kerry L. , Ruelcke Jayde E. , Petelin Lara , Moon Hyeongsun , Choi Eunju , Rae James , Blumenthal Antje , Hutmacher Dietmar , Saunders Nicholas A. , Stow Jennifer L. , Parton Robert G. , Hill Michelle M. 
Published: June 2014

BackgroundTumour‐derived extracellular vesicles (EVs) play a role in tumour progression; however, the spectrum of molecular mechanisms regulating EV secretion and cargo selection remain to be fully elucidated. We have reported that cavin‐1 expression in prostate cancer PC3 cells reduced the abundance of a subset of EV proteins, concomitant with reduced xenograft tumour growth and metastasis.MethodsWe examined the functional outcomes and mechanisms of cavin‐1 expression on PC3‐derived EVs (PC3‐EVs).ResultsPC3‐EVs were internalized by osteoclast precursor RAW264.7 cells and primary human osteoblasts (hOBs) in vitro, stimulating osteoclastogenesis 37‐fold and hOB proliferation 1.5‐fold, respectively. Strikingly, EVs derived from cavin‐1‐expressing PC3 cells (cavin‐1‐PC3‐EVs) failed to induce multinucleate osteoblasts or hOB proliferation. Cavin‐1 was not detected in EVs, indicating an indirect mechanism of action. EV morphology, size and quantity were also not affected by cavin‐1 expression, suggesting that cavin‐1 modulated EV cargo recruitment rather than release. While cavin‐1‐EVs had no osteoclastogenic function, they were internalized by RAW264.7 cells but at a reduced efficiency compared to control EVs. EV surface proteins are required for internalization of PC3‐EVs by RAW264.7 cells, as proteinase K treatment abolished uptake of both control and cavin‐1‐PC3‐EVs. Removal of sialic acid modifications by neuraminidase treatment increased the amount of control PC3‐EVs internalized by RAW264.7 cells, without affecting cavin‐1‐PC3‐EVs. This suggests that cavin‐1 expression altered the glycosylation modifications on PC3‐EV surface. Finally, cavin‐1 expression did not affect EV in vivo tissue targeting as both control and cavin‐1‐PC3‐EVs were predominantly retained in the lung and bone 24 hours after injection into mice.DiscussionTaken together, our results reveal a novel pathway for EV cargo sorting, and highlight the potential of utilizing cavin‐1‐mediated pathways to attenuate metastatic prostate cancer.
Une évaluation de la taille de l'économie informelle par un système complet de demande estimé sur données monétaires et temporelles
Authors: Aktuna-Gunes Armagan T. , Starzec Christophe , Gardes François 
Published: June 2014

Afin d’estimer la taille de l’économie informelle en Turquie, nous estimons un système de demande basé sur l’analyse du comportement de consommation proposée par Pissarides, Weber [1989], Lyssiotou et al. [2004] et Fortin et al . [2009]. Cette estimation est effectuée sur les dépenses monétaires et sur la somme des dépenses monétaires et temporelles (consacrées aux activités domestiques des ménages). Les informations nécessaires sur les inputs monétaires et temporels dans les dépenses de consommation des ménages sont obtenues par l’appariement statistique des enquêtes turques sur le Budget des familles avec l’enquête sur l’Emploi du temps [2006]. Comme prévu, la taille de l’économie informelle en Turquie estimée en utilisant les dépenses complètes (temps plus monnaie) est plus élevée que celles obtenues par l’approche des dépenses monétaires (respectivement 40,6 % et 33,5 % du pib pour les travailleurs indépendants et 20,7 % et 14,1 % du pib pour les salariés) et également plus élevée que celle obtenue par les méthodes macroéconomiques plus conventionnelles (35,1 %).
Inês de Castro, la Reine morte : mythe et réalité
Authors: Aranjo Daniel 
Published: June 2014

Cette étude poursuit les objectifs suivants : retrouver la vérité, ou ce qui reste de vérité ou de vraisemblance historique autour du personnage, d’autant que le mythe infiltre constamment ce qui nous est présenté de quatrième ou dixième main comme l’histoire d’Inês, y compris par des ouvrages de référence de type scolaire ou universitaire, tant en France qu’en Ibérie, de façon à mieux mesurer l’écart qui sépare le mythe (saisi en sa fondamentale variabilité) de ce que l’on peut retrouver ou inférer avec prudence de cette vérité historique ; appliquer une démarche mythocritique actualisée au thème d’Inês de Castro.
La secrète revendication d’une sexualité féminine : les chants du marcanda chantés par des femmes songhay-zarma d’origine captive (Niger)
Authors: Bornand Sandra 
Published: June 2014

Chez les Songhay-Zarma du Niger se déroule, durant les mariages polygames, une cérémonie particulière, le marcanda, au cours de laquelle des femmes d’origine captive évoquent, en chansons et en l’absence des hommes, la sexualité, ses plaisirs et ses déviances. Ces énonciatrices particulières abordent, dans leurs chants, des sujets tabous et semblent prendre systématiquement à contre-pied les valeurs prônées par la société.Maniant à la fois parole directe (emploi de termes vulgaires) et indirecte (métaphores, jeux sur les voix énonciatives, etc.), les chanteuses créent une complicité avec les femmes de l’auditoire, qui entendent – à travers les voix de captives – une parole qu’elles ne pourraient prononcer. Car les seules chanteuses autorisées à chanter ces chants se situent doublement au bas de l’échelle sociale : en tant que femmes et en tant que descendantes de captives qui – dit-on et contrairement aux êtres d’origine libre – « ne connaissent pas la pudeur ».
Le réalisme spéculatif à l'épreuve de la donation
Authors: Vinolo Stéphane 
Published: June 2014

Bien qu’il ne soit cité explicitement qu’une seule fois dans Après la finitude, Jean-Luc Marion apparaît comme l’une des cibles privilégiées de la démonstration de Meillassoux. Il l’est non seulement à titre de phénoménologue ayant réduit le dehors à un dehors claustral sans véritable ailleurs ; il l’est en plus en tant que paradigme des penseurs qui ont fermé tout accès rationnel à Dieu. Pourtant, en pensant une donation non-réciproque, Marion pourrait bien s’arracher à la corrélation et poser un Grand dehors, absolutoire plus qu’absolu. En utilisant Descartes comme pont entre Quentin Meillassoux et Jean-Luc Marion, nous nous proposons de montrer que le réalisme spéculatif est moins éloigné qu’il ne le pense de la phénoménologie de la donation.
Murine Spleen Tissue Regeneration from Neonatal Spleen Capsule Requires Lymphotoxin Priming of Stromal Cells
Authors: Tan Jonathan K. H. , Watanabe Takeshi 
Published: June 2014

Abstract
Spleen is a tissue with regenerative capacity, which allows autotransplantation of human spleen fragments to counteract the effects of splenectomy. We now reveal in a murine model that transplant of neonatal spleen capsule alone leads to the regeneration of full spleen tissue. This finding indicates that graft-derived spleen stromal cells, but not lymphocytes, are essential components of tissue neogenesis, a finding verified by transplant and regeneration of Rag1KO spleen capsules. We further demonstrate that lymphotoxin and lymphoid tissue inducer cells participate in two key elements of spleen neogenesis, bulk tissue regeneration and white pulp organization, identifying a lymphotoxin-dependent pathway for neonatal spleen regeneration that contrasts with previously defined lymphotoxin-independent embryonic spleen organogenesis.
GM-CSF but Not IL-17 Is Critical for the Development of Severe Interstitial Lung Disease in SKG Mice
Authors: Shiomi Aoi , Usui Takashi , Ishikawa Yuki , Shimizu Masakazu , Murakami Kosaku , Mimori Tsuneyo 
Published: June 2014

Abstract
Interstitial lung disease (ILD) is a common complication and sometimes a prognostic factor of connective tissue diseases (CTDs) in humans. However, suitable animal model of severe CTD-associated ILD (CTD-ILD) has been limited. In this study, we showed that zymosan-treated SKG mice developed not only arthritis but also chronic–progressive ILD with high mortality over several months. The pathological and clinical features of ILD in zymosan-treated SKG mice were similar to that of human severe CTD-ILD. ILD in this mouse was characterized by massive infiltration of Th17 cells, GM-CSF–producing CD4+ T cells, and CD11b+ Gr1+ neutrophils with fibrosis. Naive SKG T cells were skewed to differentiate into GM-CSF–producing cells, and GM-CSF secreted by T cells enhanced IL-6 and IL-1β production by macrophages, which in turn enhanced differentiation of IL-17A– and/or GM-CSF–producing T cells and infiltration of neutrophils into lung. Neutralization of GM-CSF completely blocked the development of this ILD, and the blocking of IL-6 signaling resulted in partial prevention of it, whereas neutralization of IL-17A did not. In contrast, the progression of arthritis was inhibited by the neutralization of GM-CSF and slightly by the neutralization of IL-17A, but not by the blocking of IL-6 signaling. These data suggested zymosan-treated SKG mice could be a useful mouse model of severe CTD-ILD, and GM-CSF, rather than IL-17A or IL-6, contributed to the development of ILD in zymosan-treated SKG mice, indicating that neutralization of GM-CSF would be a useful therapeutic strategy for severe CTD-ILD.
Klebsiella pneumoniaeAlleviates Influenza-Induced Acute Lung Injury via Limiting NK Cell Expansion
Authors: Wang Jian , Li Fengqi , Sun Rui , Gao Xiang , Wei Haiming , Tian Zhigang 
Published: June 2014

AbstractA protective effect induced by bacterial preinfection upon a subsequent lethal influenza virus infection has been observed, but the underlying immune mechanisms have not yet been fully elucidated. In this study, we used a mouse model of Klebsiella pneumoniae preinfection to gain insight into how bacterial preinfection influences the subsequent lethal influenza virus infection. We found that K. pneumoniae preinfection significantly attenuated lung immune injury and decreased mortality during influenza virus infection, but K. pneumoniae–specific immunity was not involved in this cross-protection against influenza virus. K. pneumoniae preinfection limited NK cell expansion, which was involved in influenza-induced immune injury and death. Furthermore, K. pneumoniae preinfection could not control NK cell expansion and death during influenza virus infection in Rag1−/− mice, but adoptive transfer of T cells from wild-type mice was able to restore this protective effect. Our data suggest that the adaptive immune response activated by bacterial infection limits the excessive innate immune response induced by a subsequent influenza infection, ultimately protecting mice from death.
Mode of Delivery Shapes Gut Colonization Pattern and Modulates Regulatory Immunity in Mice
Authors: Hansen Camilla H. F. , Andersen Line S. F. , Krych Łukasz , Metzdorff Stine B. , Hasselby Jane P. , Skov Søren , Nielsen Dennis S. , Buschard Karsten , Hansen Lars H. , Hansen Axel K. 
Published: June 2014

Abstract
Delivery mode has been associated with long-term changes in gut microbiota composition and more recently also with changes in the immune system. This has further been suggested to link Cesarean section (C-section) with an increased risk for development of immune-mediated diseases such as type 1 diabetes. In this study, we demonstrate that both C-section and cross-fostering with a genetically distinct strain influence the gut microbiota composition and immune key markers in mice. Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-based 16S rRNA gene amplicon sequencing revealed that mice born by C-section had a distinct bacterial profile at weaning characterized by higher abundance of Bacteroides and Lachnospiraceae, and less Rikenellaceae and Ruminococcus. No clustering according to delivery method as determined by principal component analysis of denaturing gradient gel electrophoresis profiles was evident in adult mice. However, the adult C-section–born mice had lower proportions of Foxp3+ regulatory T cells, tolerogenic CD103+ dendritic cells, and less Il10 gene expression in mesenteric lymph nodes and spleens. This demonstrates long-term systemic effect on the regulatory immune system that was also evident in NOD mice, a model of type 1 diabetes, born by C-section. However, no effect of delivery mode was seen on diabetes incidence or insulitis development. In conclusion, the first exposure to microorganisms seems to be crucial for the early life gut microbiota and priming of regulatory immune system in mice, and mode of delivery strongly influences this.
Death, Taxes and Advance Directives
Authors: D‘Amore J.D. , Jones S.L. , Sittig D.F. , Ness R.B. , Wood N.M. 
Published: June 2014

SummarySuboptimal care at the end-of-life can be due to lack of access or knowledge of patient wishes. Ambiguity is often the result of non-standardized formats. Borrowing digital technology from other industries and using existing health information infrastructure can greatly improve the completion, storage, and distribution of advance directives. We believe several simple, low-cost adaptations to regional and federal programs can raise the standard of end-of-life care.
Citation: Wood NM, D’Amore JD, Jones SL, Sittig DF, Ness RB. Death, taxes and advance directives. Appl Clin Inf 2014; 5: 589–593 http://dx.doi.org/10.4338/ACI-2013-12-IE-0099
Ophthalmic Manifestations in Familial Mediterranean Fever: A Case Series of 6 Patients
Authors: Yazici Alper , Ozdal Pinar , Yuksekkaya Pinar , Elgin Ufuk , Teke Mehmet Yasin , Sari Esin 
Published: June 2014

Purpose To describe the ocular involvement of patients with familial Mediterranean fever (FMF) followed in a tertiary referral center. Methods The data of 6 patients with FMF were retrospectively reviewed. Detailed ophthalmologic examinations, type of inflammation, course of the disease, number of recurrences, treatment regimens, complications, and comorbid ocular or systemic diseases were noted. Results The mean age ± SD at diagnosis was 29.3 ± 19.3 (4-53) years. A total of 66.7% of the patients were male and 66.7% of the patients had bilateral disease. The anatomical distribution of the ophthalmic involvement was as follows: posterior uveitis in 2 (33.3%), anterior uveitis in 2 (33.3%), posterior scleritis in 1 (16.7%), and intermediate uveitis in 1 (16.7%) patient. The course was recurrent in 50% of the patients. Final visual acuities were favorable except in the patients with chronic course uveitis. Cystoid macula edema, epiretinal membrane, retinal ischemia, cataract, glaucoma, and band keratopathy were complications noted in the follow-up period. Both cataract and glaucoma patients (50%) needed a surgical intervention. In 33.3% of patients, Behçet disease was present as a concurrent disease. In patients with posterior uveitis and the patient with intermediate uveitis (50%), systemic immunosuppression was required. Conclusions There was a male and bilateral involvement predominance. The course of the inflammation was recurrent in half of the patients. Since ocular involvement in FMF is very rare, it should be considered as diagnosis of exclusion.
Outcome of 110 Basal Cell Carcinomas of the Eyelid Treated with Frozen Section–Controlled Excision: Mean Follow-up over 5 Years
Authors: Resti Antonio Giordano , Sacconi Riccardo , Baccelli Nicola , Bandello Francesco 
Published: June 2014

Purpose To analyze the outcome and risk factors of recurrence in patients with basal cell carcinomas (BCCs) of the eyelid treated by en face frozen section–controlled (FSC) excision with a mean follow-up over 5 years. Methods This was a retrospective series of 108 patients with 110 biopsy-proven eyelid BCCs. All lesions were excised with 2 mm margins clinically free from neoplasia at clinical examination. For each tumor, en face frozen section examination of surgical margins was employed for the histologic confirmation before the reconstruction. Subsequently, all margins were submitted for permanent paraffin sections. Results Of 110 malignancies, 80.9% represented primary carcinomas and 19.1% secondary ones. The overall recurrence rate was 1.8%, with a mean follow-up of 72.4 months (range 30–167). The mean time between the excision of the lesion and the diagnosis of the recurrence was 24 months (range 20–28). No recurrences were observed in 62 tumors followed up for at least 5 years. Secondary BCCs were associated with a higher recurrence rate compared with primary BCCs (4.8% and 1.1%, respectively, p = 0.262). Conclusions The FSC excision of eyelid BCCs yields recurrence rates comparable to those of Mohs micrographic surgery at 5-year follow-up. Intraoperative microscopic margin control improves the cure rate of eyelid BCCs, and FSC excision with small margins (2 mm) clinically free from neoplasia is associated with easier reconstruction and better cosmetic and functional outcomes.
High Magnification in vivo Evaluation of the Mechanism of Failure of An Ex-PRESS Shunt Implanted Under the Sclera Flap
Authors: Holló Gábor , Naghizadeh Farzaneh 
Published: June 2014

Purpose To investigate the mechanism of fibrosis-related failure of an Ex-PRESS shunt implanted under the scleral flap in trabeculectomy. Methods Case report. Results A patient with end-stage secondary open-angle glaucoma on his only functioning right eye was referred for glaucoma valve implantation. His right eye had undergone congenital cataract surgery in early childhood and posterior chamber lens implantation 20 years ago. During the last 18 months, the right eye underwent pars plana vitrectomy with silicone oil implantation, silicone oil removal, 2 sessions of cyclophotocoagulation, 2 failed trabeculectomies (one of them combined with Ex-PRESS shunt implantation under the scleral flap), and 4 trabeculectomy revisions. At presentation, the intraocular pressure varied between 47 and 52 mm Hg on maximal systemic and topical intraocular pressure–lowering medication. Serial limbus parallel scans made with the CAM-L cornea lens adapter of the Optovue Fourier-domain optical coherence tomograph revealed that the space between the inner surface of the scleral flap and the external orifice of the Ex-PRESS shunt was filled by scar tissue, and the scar tissue appeared to invade the lumen of the shunt. Conclusions The findings suggest that failure of an Ex-PRESS shunt is not always caused by episcleral fibrosis but may also be caused by scar formation between the scleral flap and the implant, inside the surgical sclera tunnel.
Clinical Outcomes with a New Trifocal Intraocular Lens
Authors: Law Elizabeth M. , Aggarwal Rajesh K. , Kasaby Hosam 
Published: June 2014

Purpose To evaluate visual and refractive outcomes, contrast sensitivity, and quality of vision after cataract surgery with the implantation of a new modality of trifocal intraocular lens (IOL). Methods This case series comprised 30 patients who had bilateral implantation of the AT LISA tri 839 MP trifocal IOL after phacoemulsification for either cataract or refractive lens exchange surgery. Uncorrected (UDVA) and corrected monocular distance visual acuity (CDVA) using a logMAR chart, binocular uncorrected (UNVA) and corrected near visual acuity (CNVA) (40 cm and patients' preferred distance) using the Radner Reading Charts, distance-corrected intermediate visual acuity (DCIVA) (70 cm), a binocular defocus curve, contrast sensitivity (CS) with the Pelli-Robson test, and subjective quality of vision by a short questionnaire were evaluated at 1, 3, and 6 months postoperatively. Results At 6 months, mean values of 0.05 ± 0.07 and −0.02 ± 0.05 logMAR were obtained for monocular UDVA and CDVA, respectively. Mean values of 0.16 ± 0.07, 0.12 ± 0.07, and 0.16 ± 0.07 logRAD were obtained for binocular UNVA, CNVA, and DCIVA, respectively. Significant changes were only detected in monocular CDVA (p<0.01) and UDVA that improved between 3 and 6 months postoperatively (p = 0.01). A visual acuity level of 0.2 logRAD could be seen in binocular defocus curves between +1.00 and −3.00 D. At 6 months, mean photopic and mesopic CS were 1.52 ± 0.11 and 1.54 ± 0.11. Regarding the questionnaire, almost all patients were satisfied with their distance, intermediate, and near vision. Difficulties associated with photic phenomena decreased significantly over time. Conclusions The AT LISA trifocal IOL provides excellent uncorrected distance, intermediate, and near visual outcomes.
Nasolacrimal Duct Obstruction Secondary to Dental Impaction
Authors: Resti Antonio Giordano , Bertazzoni Giacomo , Trimarchi Matteo 
Published: June 2014

Purpose To describe a patient with nasolacrimal duct obstruction (NLDO) caused by dental impaction. Methods This is an interventional case report of an 18-year-old man presenting epiphora of the right eye. Previous surgical management was unsuccessful. Computed tomography (CT) of the orbits and sinuses was performed. Dacryocystorhinostomy (DCR) on the right lacrimal sac resulted in complete remission of symptoms. The DCR surgical outcome at last follow-up was recorded. Results The CT imaging showed ankylosis of deciduous canines and impaction of persistent canines. The root of the right impacted dental element was involved with the nasolacrimal duct, leading to NLDO. Endoscopic DCR resulted in complete remission of symptoms. The patient was symptom-free at last follow-up. Conclusions Our case adds NLDO to the long list of complications arising from dental impactions, highlighting the importance of correct early management of the latter condition. This case also shows that dental impaction can be included among the secondary mechanical causes of NLDO.
Detecting an Event of Progression Using Glaucoma Probability Score and the Stereometric Parameters of Heidelberg Retina Tomograph 3
Authors: Saarela Ville , Falck Aura , Tuulonen Anja 
Published: June 2014

Purpose To evaluate the correlation of the change in glaucoma probability score (GPS) and the stereometric optic nerve head (ONH) parameters of the Heidelberg Retina Tomograph (HRT)3 to an event of glaucomatous progression observed with stereoscopic ONH photography. Methods The subjects for this retrospective follow-up study were monitored with the HRT and stereoscopic ONH photographs. Stable, high-quality imaging and at least 18 months of follow-up was required. The topography examinations were acquired using HRT II and calculated with HRT3 software. The event of progression was determined by masked evaluation of stereoscopic ONH photographs. Results A total of 476 eyes of 342 subjects met the inclusion criteria. All the examinations with HRT II were backwards compatible with either the GPS or the stereometric parameters of HRT3. The highest statistical significance for the correlation with progression was observed in the change in cup volume and cup:disc area ratio (p<0.0005). The vertical cup:disc ratio was the parameter with the largest area under the receiver operating characteristics curve (AUC = 0.696). The AUC calculated for the change in GPS was 0.541. The GPS showed no statistically significant correlation with progression (p = 0.213). Conclusions Detecting an event of glaucomatous progression should not be based solely on the change in GPS or the stereometric parameters of the HRT. The good backwards compatibility between HRT II and HRT3 should allow the detection of a trend of glaucomatous progression even when changing from HRT II to HRT3 during the follow-up of glaucoma.