bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Imaging of MicroRNA‐21 In Vivo for Real‐Time Monitoring of the Cancer Initiation and Progression
Authors: Zheng Liting , Wu Ying , Wang Qian , Du Wei , Chen Lanlan , Song Jibin , Yang Huanghao 
Published: August 2024

AbstractMicroRNA‐21 (MiR‐21) has been confirmed to be upregulated in tumors, and its abnormal expression is closely associated with tumor occurrence. However, the traditional imaging methods are limited to qualitative imaging of miR‐21, and no effective strategy has been developed for monitoring its concentration in vivo during cancer initiation and progression. Herein, a biosensor is created utilizing a NIR‐II ratiometric fluorescent nanoprobe to quantitatively monitor dynamic alterations in miR‐21 levels in vivo. The nanoprobe (termed DCNP@DNA2@IR806) is constructed by introducing IR806 as a donor and down‐conversion nanoparticles (DCNP) as the acceptor, using DNA as linkers. Upon miR‐21‐responsive initiation of the nanoprobe, the 1550 nm fluorescent signal of DCNP stimulated by a 808 nm laser (F1550, 808Ex) increased because of the close proximity of IR806 to the DCNP and the subsequent non‐radiative energy transfer (NRET). Meanwhile, the 1550 nm fluorescent signal of DCNP stimulated by a 980 nm laser (F1550, 980Ex) remained stable because of the absence of NRET. This ratiometric NIR‐II fluorescent signal has been confirmed to be a reliable indicator of miR‐21 concentration in vivo. The strategy holds promise for further enhancing the understanding of microRNAs‐based molecular mechanisms underlying cancer progression, laying a foundation for the early diagnosis of microRNAs‐related diseases.
Spider Silk: Rapid, Bottom‐Up Self‐Assembly of MaSp1 into Hierarchically Structured Fibers Through Biomimetic Processing
Authors: Malay Ali D. , Oktaviani Nur Alia , Chen Jianming , Numata Keiji 
Published: August 2024

AbstractSpider dragline silk's exceptional mechanical performance, coupled with its benign production pathway, have inspired the design of diverse smart materials. Remarkably, relatively little is known about the self‐assembly process of major ampullate spidroin 1 (MaSp1) – the main protein constituent of dragline fiber – during its rapid conversion from soluble precursor protein into hierarchically structured mature silk fibers. Herein, the biomimetic potential of MaSp1 is explored using a new recombinant platform with full domain representation. MaSp1 is found to undergo efficient liquid‐liquid phase separation (LLPS) in response to kosmotropic ions, with notably higher propensity compared to MaSp2, and with MaSp1 exhibiting a more complex solubility profile relative to levels of sodium chloride. To further the understanding of silk spinning, the rapid assembly of mesoscale structures is monitored in real‐time in response to ion and pH gradients, revealing a progression from LLPS droplets toward aligned hierarchical fibers. Furthermore, using this biomimetic system, insoluble macroscopic MaSp1 fibers can be readily generated, wherein the application of mechanical deformation triggers the emergence of β‐sheet secondary structures. The insights gained from this study can have broader application in efforts to mimic the formation of biomaterials with spatially organized features across multiple length scales.
Boosting Ferroptosis and immunotherapy for Colorectal Cancer by Lactate‐Related Metabolic Reprogramming
Authors: Li Zhenhao , He Shan , Xie Lei , Zeng Guoning , Huang Jiehao , Wang Huaiming , Chen Hongwu , Deng Tingting , Xia Yubin , Huang Cong , Chen Zhian 
Published: August 2024

AbstractAs the presence of anaerobic metabolism of glucose, solid tumors characteristically display higher levels of lactate production, which attenuate ferroptosis therapy and the subsequent anti‐tumor immune response. Herein, hyaluronic acid (HA)‐modified and lactate oxidase (LOX)‐loaded nanoscale metal organic frameworks (MOFs), termed as FCS@LOX@HA (FCSLH) is constructed, achieving tumor‐targeted metabolic combined chemo‐dynamic therapy (CDT). Notably, the high LOX‐loading capacity of MOFs is achieved by adjusting the Fe/Cu ratio. Upon internalization by cancer cells, the FCS MOFs reacted with local glutathione (GSH) to release Fe/Cu ions for CDT. Meanwhile, the LOX catalyzed endogenous lactate to pyruvate accompanied with a release of H2O2; while the latter can enhance the FCS MOFs‐mediated CDT effect. Additionally, lactate exhaustion can impair the antioxidant system by inhibiting the HIF‐1α/SLC1A1 pathway, resulting in the accumulation of lipid peroxidation, and ferroptosis occurs accompanied by immunogenic cell death. Furthermore, lactate exhaustion within tumor‐associated macrophages (TAMs) can inhibit M2 macrophage polarization by suppressing the NF‐κB/HIF‐1α pathway, thereby augmenting anti‐tumor immune response. The in vivo studies demonstrated that cooperating with PD‐L1 antibodies can achieve excellent anti‐tumor therapeutic efficacy. Taken together, FCSLH can amplify the ferroptosis‐mediated immune response through simultaneous dysfunction of cancer cells and TAMs, which provides novel insight for integrating metabolic programmed therapy and immunotherapy.
Core–Shell Composite Nanofibers with High Temperature Resistance, Hydrophobicity and Breathability for Efficient Daytime Passive Radiative Cooling
Authors: Fan Hong , Wang Kefan , Ding Yangjian , Qiang Yueyue , Yang Zhuo , Xu Huan , Li Min , Xu Zewen , Huang Cheng 
Published: August 2024

AbstractRadiative cooling technology, which is renowned for its ability to dissipate heat without energy consumption, has garnered immense interest. However, achieving high performance, multifunctionality, and smart integration while addressing challenges such as film thickness and enhancing anisotropic light reflection remains challenging. In this study, a core–shell composite nanofiber, PVDF@PEI, is introduced and designed primarily from a symmetry‐breaking perspective to develop highly efficient radiative cooling materials. Using a combination of solvent‐induced phase separation (EIPS) inverse spinning and (aggregation) self‐assembly methods (EISA or EIAA) and coaxial electrostatic spinning (ES), superconformal surface anisotropic porous nanofiber membranes are fabricated. These membranes exhibit exceptional thermal stability (up to 210 °C), high hydrophobicity (contact angle of 126°), robust UV protection (exceeding 99%), a fluorescence multiplication effect (with a 0.6% increase in fluorescence quantum efficiency), and good breathability. These properties enable the material to excel in a wide range of application scenarios. Moreover, this material achieved a remarkable daytime cooling temperature of 8 °C. The development of this fiber membrane offers significant advancements in the field of wearables and the multifunctionality of materials, paving new paths for future research and innovation.
Simultaneous Color‐ and Dose‐Controlled Thiol–Ene Resins for Multimodulus 3D Printing with Programmable Interfacial Gradients
Authors: Kiker Meghan T. , Recker Elizabeth A. , Uddin Ain , Page Zachariah A. 
Published: August 2024

AbstractDrawing inspiration from nature's own intricate designs, synthetic multimaterial structures have the potential to offer properties and functionality that exceed those of the individual components. However, several contemporary hurdles, from a lack of efficient chemistries to processing constraints, preclude the rapid and precise manufacturing of such materials. Herein, the development of a photocurable resin comprising color‐selective initiators is reported, triggering disparate polymerization mechanisms between acrylate and thiol functionality. Exposure of the resin to UV light (365 nm) leads to the formation of a rigid, highly crosslinked network via a radical chain‐growth mechanism, while violet light (405 nm) forms a soft, lightly crosslinked network via an anionic step‐growth mechanism. The efficient photocurable resin is employed in multicolor digital light processing 3D printing to provide structures with moduli spanning over two orders of magnitude. Furthermore, local intensity (i.e., grayscale) control enables the formation of programmable stiffness gradients with ≈150× change in modulus occurring across sharp (≈200 µm) and shallow (≈9 mm) interfaces, mimetic of the human knee entheses and squid beaks, respectively. This study provides composition–processing–property relationships to inform advanced manufacturing of next‐generation multimaterial objects having a myriad of applications from healthcare to education.
Viable Approach of Tuning Oxide Semiconductor Thin Films in Solution‐Processed Heterojunction Thin Films Transistors for Both Higher Performances and Stability
Authors: Eun Jun‐Su , Lee Jinuk , Na Jeong‐Hyeon , Park Jun‐Hyeong , Park Won , Feng Junhao , Seo Kyung‐Ho , Jang Jaewon , Kang In Man , Kim Do‐Kyung , Bae Jin‐Hyuk 
Published: August 2024

AbstractMetal‐oxide thin‐film transistors (TFTs) have garnered much attention because of their advantages such as high transparency, low leakage current, and low processing temperature. However, there is a need to continuously improve their mobility and bias stability for application to next‐generation advanced electronics. In this study, the thickness of bilayer semiconductors is finely controlled to enhance the charge transport characteristics and bias stability in solution‐processed heterojunction oxide TFTs. The thicknesses of the top and bottom layers in the bilayer are individually adjusted by controlling solution molarity. The introduction of a bilayer channel improved the electrical performance of oxide TFTs via effective charge transport. However, trap‐limited conduction becomes dominant in the bilayer with an excessively thick top layer, thereby leading to a significant reduction in mobility and positive bias stability. Meanwhile, although increasing the bottom layer thickness contributes to improved mobility and reliability, it causes a serious negative shift in threshold voltage (VTH). TFTs with an optimized bilayer structure show high mobility at a VTH close to 0 V and have particularly excellent positive bias stress stability. This study on bilayer channel thickness will be beneficial for developing advanced transistors with optimized bilayer or multilayer channels.
Accelerated solvent extraction of apomorphine from Nymphaea caerulea (Blue Water Lily) products: A proof‐of‐concept Green extraction for plant materials
Authors: Krishna Rohith , Dixit Anirudha , Patil Ketan , Agrawal Shalvi , Joseph Jilja , Pandey Astha , Sankhla Mahipal Singh 
Published: August 2024

AbstractNymphaea caerulea (Blue water lily) is an esthetically pleasing aquatic plant which is widely located across India and Africa. The blue water lily contains an alkaloid called apomorphine which is said to be a sedative, and a nonselective dopamine agonist and is now available in the local and online market in the form of powders and oils for various applications such as sleeping aid, anxiety reliever and sexual performance enhancer. These properties are abused by the consumption of Nymphaea caerulea to achieve a state of “high” which has led the categorization of the same as a novel psychoactive substance. In this paper, a rapid mass spectral analysis was performed for the preliminary screening of commercially available blue water lily products using the Waters Radian as soon as possible instrument, followed by the high performance liquid chromatography‐photo diode array method development and validation of the samples for the qualitative and quantitative analysis of apomorphine. Accelerated solvent extraction as a green alternative to the conventional soxhlet extraction was used in the extraction of the plant material. The method was finally screened for its greenness using the Complex green analytical procedure index method. The method was validated with a linearity of 0.9973; limit of detection and limit of quantitation of 0.02 and 0.18 µg/mL, respectively. The method was able to detect and quantitate apomorphine in two samples from the commercially available natural products of Nymphaea caerulea.
Absence of developmental and reproductive toxicity in rats, rabbits, and zebrafish embryos exposed to antimalarial drug cabamiquine
Authors: Gado Andreas , Hewitt Philip , Ballard Peter , Tornesi Belen , Baeurle Tobias Hyun Ho , Oeuvray Claude , Spangenberg Thomas , Demarta‐Gatsi Claudia 
Published: August 2024

AbstractBackgroundWhen developing new antimalarial drugs, considering their potential use during pregnancy as preventive or curative therapy is crucial. This prevents the parasite from affecting embryonic development and reduces maternal and fetal death risks. Consequently, understanding the exposure and safety of antimalarial drugs during pregnancy is crucial, with well‐designed animal studies playing a key role in this assessment.MethodsAs part of the drug development program for cabamiquine, a series of developmental and reproductive toxicity studies were conducted in rats and rabbits. Additionally, the zebrafish embryo model was used to further improve embryo exposure, minimize confounding factors related to maternal toxicity, and assess developmental risks of cabamiquine.ResultsIn these studies, although maternal toxicity was observed, there were no cabamiquine‐related adverse effects on fertility, embryonic, or fetal development at maternal exposures representing significant multiples (up to five and 10 times higher in rabbit and rats, respectively) than the exposure at the anticipated efficacious human dose. Similarly, no adverse effects were observed on ZF embryonic development, even though cabamiquine concentrations in the embryos were 10‐fold higher than nominal concentrations.ConclusionsThe results obtained in a full set of reproductive toxicity studies did not provide evidence of detrimental effects on the conceptuses and progeny at maternally nontoxic doses and exposures, still representing a multiple of the anticipated systemic exposures in women of childbearing potential (WOCBP). Cabamiquine can therefore be considered a suitable therapeutic option for WOCBP and pregnant women living in malaria‐endemic regions by significantly reducing maternal and infant malaria death rates.
Relationship between sustainability disclosure and corporate reputation: Evidence from a meta‐analysis
Authors: Pajuelo‐Moreno María Luisa , Barroso‐Méndez María Jesús , Gallardo‐Vázquez Dolores 
Published: August 2024

AbstractPrevious studies on the connection between sustainability disclosure (SD) and corporate reputation (CR) yielded varying results. This study presents a quantitative analysis of the impact of SD on CR, based on previous empirical studies, while determining the influence of substantive (SD and CR measures), methodological (unit and type of analysis), and extrinsic (number of countries, country legal system, country development, and continents) moderators. Specifically, by employing a meta‐analytical approach grounded in a systematic review of the literature, a total of 92 empirical research articles were analyzed. The results confirmed a significant positive correlation between SD and CR, which was greatly affected by the moderation exercised by the measures of reputation of the entity, contextual country differences, and methodological characteristics.
CT‐derived simulations to predict outcomes in patients undergoing transcatheter aortic valve implantation with an ACURATE Neo2 valve the PRECISE‐TAVI cohort B trial
Authors: Hokken Thijmen W. , Nuyens Philippe , Ruffo Claudio , Nuis Rutger‐Jan , Daemen Joost , Kardys Isabella , Budde Ricardo , Buzzatti Nicola , de Backer Ole , Van Mieghem Nicolas M. 
Published: August 2024

AbstractBackgroundParavalvular leakage (PVL) and conduction disorders that require permanent pacemaker implantation (PPI) remain clinically relevant challenges after transcatheter aortic valve implantation (TAVI). Computed tomography‐based simulations may predict the risk of significant PVL and PPI.AimsTo evaluate the feasibility and accuracy of preprocedural computer simulation with FEops HEARTguide™ to predict >trace PVL and PPI after TAVI with the self‐expanding supra‐annular ACURATE Neo2 transcatheter heart valve.MethodsProspective multicenter observational study that included consecutive patients undergoing TAVI with an ACURATE Neo2 valve. Computer simulations were performed before the TAVI procedure as part of the preprocedural planning. Follow‐up period for PPI and PVL was 30 days.ResultsSixty‐five patients were included (median age 81 years (25th−75th percentile 77–84.5)). New left bundle branch block occurred in five patients (7.7%) and PPI in two patients (3%). Contact pressure index (CPI) was similar for patients with vs without new conduction disorders. Patients with PPI had numerically higher CPI than those without PPI (median CPI 20.0% (25th−75th percentile 15.0–25.0) vs. 13.0% (25th−75th percentile 5.5–18), p = 0.27). More than trace PVL occurred in 30%. Median PVL was significantly lower in patients with none‐trace PVL (3.2 mL/s [25th−75th percentile 2.2–5.0]), compared to mild PVL (5.2 mL/s [25th−75th percentile 3.2–10.3]) and moderate PVL (12.6 mL/s [25th−75th percentile 3.9–21.3])(p = 0.036). A simulated PVL‐cutoff of 9.65 mL/s identified patients with >trace PVL (AUC 0.70 (95% CI 0.55–0.85), sensitivity 42%, specificity 95%).ConclusionIn our study FEops HEARTguide™ simulations identified patients at risk for >trace PVL with ACURATE Neo2 TAVI but not for PPI.
Current status and perspectives in environmental oncology
Authors: Liu Jie , Gan Ting , Hu Wenbiao , Li Yumin 
Published: August 2024

AbstractCancer stands as a leading global cause of death, with its etiology characterized by complexity and multifaceted factors. Growing research indicates a strong correlation between environmental factors and cancer incidence, underscoring the critical importance of intervening in environmental risk factors to mitigate cancer occurrence. Despite this, specialized research institutions focusing on the intersection of environment and cancer remain scarce, with global investment in cancer prevention significantly trailing behind efforts in diagnosis and treatment. Against the backdrop of rapid global climate change, industrialization, urbanization, aging populations, and the globalization of lifestyles, we proposed the concept of Environmental Oncology (EO) to address these challenges. We discussed the rationale and necessity of developing EO and presented a comprehensive research framework focusing on cancer prevention and treatment. Future EO research will aim to identify cancer causes and implement early prevention strategies using advanced scientific technologies and methods. By emphasizing multidisciplinary collaboration and integrating molecular biology at the micro level, EO will explore the relationship between external and internal environments and cancer. EO will identify potential therapeutic targets by studying the pathways through which environmental exposures lead to carcinogenesis. EO will develop early warning systems and disseminate research findings by collecting big data, employing robust statistical models, and establishing research centers.
N‐Heterocyclic Carbenes and N‐Heterocycles as Synthetic Reagents and Building Blocks
Authors: Jacobs Emily , Nguyen Thanh Vinh 
Published: August 2024

AbstractN‐Heterocyclic carbenes (NHCs) have become important tools in modern synthetic chemistry due to their versatility as organocatalysts and ligands in organometallic complexes. Since their first isolation and characterization, NHCs have demonstrated significant utility in various catalytic processes, offering advantages such as strong σ‐electron donation and the ability to stabilize reactive intermediates. However, beyond their well‐documented roles in catalysis, the potential of NHCs as stoichiometric reagents and synthetic building blocks remains an underexplored yet promising area. This Mini‐review aims to shed light on these lesser‐known applications of NHCs and their N‐heterocyclic precursors or derivatives in organic synthesis. Furthermore, we discuss how the unique electronic and steric properties of NHCs can be harnessed to develop new synthetic methodologies or construct interesting organic frameworks. By highlighting these emerging uses, we hope to encourage further research into the non‐catalytic applications of NHCs, broadening their scope and impact in synthetic chemistry.
Photomediated Hexafluoroisopropoxylation of Unactivated Aryl Halides
Authors: Boyden Brooke , Tobin Sydney , Haen Natalie , Metz Adia , Wojnowiak Cohen , Taylor Trevor , Cutty Alex , Litza Alex , Stiles Isaac , Soehner Nathan , Oberbroeckling Alex , O'Leary Nicole , Popp Hannah , MacKenzie Ian 
Published: August 2024

AbstractThe use of fluoroalkoxy groups, such as trifluoromethoxy and 2,2,2‐trifluoroethoxy groups, in pharmaceutical and agrochemical development has increased dramatically in recent years. However, hexafluoroisopropoxy groups have remained significantly under‐represented, presumably due to limited synthetic methods for accessing this substituent in good yields. Herein, we report a mild, photochemical nucleophilic aromatic substitution (SNAr) approach for the synthesis of hexafluoroisopropyl aryl ethers from unactivated and abundant aryl halides. Notably, aryl chloride and bromide functionality are efficiently engaged by this methodology in addition to the traditional aryl fluoride nucleofuge. This method provided access to a diverse array of hexafluoroisopropyl aryl ethers, including multiple examples of late‐stage functionalization of active pharmaceutical ingredients. A simple flow system was adapted for 10x scale‐up, maintaining good yield for the reaction. Initial mechanistic studies indicate single electron oxidation of the arene as a key step in product formation.
Fuzzy averaging level control for tight product quality control
Authors: Gupta Aayush , Srivastava Prakhar , Kaistha Nitin 
Published: August 2024

AbstractThis work develops a fuzzy averaging level controller (ALC) to mitigate flow variability while avoiding high and low level alarm limit breaches. Comparison with proportional (P) and proportional integral (PI) level controllers and their non‐linear variants demonstrates the fuzzy controller to be highly effective in mitigating flow transients for low and moderate size flow disturbances. The performance is comparable for large disturbances. Application of the developed fuzzy ALC to a ternary benzene‐toluene‐xylene direct split separation scheme as well as the separation section of a conventional cumene process demonstrates significantly superior product quality control due to flow transient variability mitigation. The product quality control variability is reduced by up to 1.5 times. The developed fuzzy ALC is therefore suitable for plant‐wide control applications.