bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescent discrimination for snake venom via a dual-mode supramolecular sensor array
Authors: Tian Han-Wen , Tian Jia-Hong , Hu Xin-Yue , Guo Dong-Sheng 
Published: February 2025

Abstract
Precise snake venom identification is the prerequisite for clinical treatment, production of antiserum, basic research, and other applications. Unfortunately, it is still a challenging task which mainly originates from their extraordinary complicated protein composition. Differential sensing strategy was applied benefiting from its advantage in discrimination of complex mixtures. Herein, A dual-mode supramolecular fluorescent sensor array based on the heteromultivalent sensing strategy was developed. The heteromultivalent macrocyclic coassemblies with strong and pan-selective binding to proteins were employed to construct sensor units. Fluorescence intensity and anisotropy signals were integrated, which were expected to show multidimensional information of proteins, such as surface groups, protein structures, and molecular weights. Finally, a supramolecular sensor array with the ability of facile, rapid and general species-specific identification and taxonomic classification for snake venom was constructed. The sensor array also demonstrated its advantage in semiquantitative analysis and multi-level identification, suggesting its great potential for practical use.
Enhancing vaginal drug delivery: the nanoemulsion gel strategy
Authors: Basu Biswajit , Ash Dipanjana , Dutta Ayon , Goswami Rajdip , Dutta Swarnali , Garala Kevin , Elossaily Gehan M. , Kapoor Devesh U. , Prajapati Bhupendra G. 
Published: February 2025

Abstract
In the 21st century, 75 % of women encounter vaginal malfunctions (cervicitis, mucoid ectopy, aerobic vaginitis, candidiasis, bacterial vaginosis, degenerated vaginitis, desquamative chronic vaginitis, and sexually transmitted infections) that clinically manifest as irritation, awkwardness, irregular bleeding, or evacuation. Vaginal drug delivery, an approach to treat the aforementioned diseases, primarily focuses on vaginal epithelium thickness, cyclic changes, vaginal secretions, mucus and enzymatic activity, pH, local microflora, and the presence of efflux transporters for their fabrication. There has been a growing interest in nanoemulsion gel for drug delivery to the vagina in comparison to conventional vaginal delivery systems (rings and gels). This review provides an in-depth examination of nanoemulsion gel formulation, highlighting key considerations such as the choice of vehicles, gel-forming agents, and permeation enhancers. It further explores formulation techniques, stability issues, and novel approaches like mucoadhesive and controlled-release systems. The biocompatibility and safety of nanoemulsion gels are also critically assessed, along with regulatory aspects. The paper underscores the growing significance of these formulations in the management of various vaginal conditions, including infections, hormonal therapies, and contraceptive applications. As nanoemulsion gels continue to evolve, their role in optimizing vaginal drug delivery systems offers considerable potential for improving therapeutic outcomes and patient compliance.
Abnormal Uterine Bleeding and External Genital Endometriosis in a 14-Year-Old Patient with Idiopathic Thrombocytopenic Purpura: Case Report
Authors: Sibirskaya Elena V. , Karachentsova Irina V. , Kurmangaleeva Aliya Yu. , Annakulieva Aina S. 
Published: February 2025

Background. Idiopathic Thrombocytopenic Purpura is one of the most common acquired blood-clotting disorders in adolescents. This disease is characterized by an increased risk of developing concomitant gynecological pathologies, such as abnormal uterine bleeding, ovarian tumors and tumor-like masses, external genital endometriosis, which requires careful medical supervision and, if necessary, surgical intervention. Case report. A clinical event of a 14-year-old patient with idiopathic thrombocytopenic purpura complicated by external genital endometriosis and abnormal uterine bleeding is being considered. The patient underwent surgery in the amount of laparoscopic oophorectomy, which prevented the development of severe complications. Conclusion. This case gives accent to the importance of a comprehensive and interdisciplinary approach to the treatment of patients with idiopathic thrombocytopenic purpura and concomitant gynecological pathologies, which helps minimize the risk of life-threatening complications.
Hypophosphatemic Rickets in Patients from Bichoric Biamniotic Twins: A Case Report
Authors: Nechaeva Anna S. , Grigoryan Emma S. , Turti Tatyana V. , Privalova Tatiana E. , Vashakmadze Nato D. , Krasnoshchekova Nina A. 
Published: February 2025

Background. X-linked dominant hypophosphatemic rickets (X-linked hypophosphatemia, XLH) is a disease caused by mutations in the PHEX gene (located at the Xp22.1 locus), which encodes an enzyme bound to the cell surface that cleaves the protein phosphate-regulating neutral endopeptidase PHEX, predominantly expressed in osteoblasts, osteocytes and teeth. This is a rare genetic disease that has difficulties in early diagnosis, in which a multidisciplinary approach is important. Currently, a new targeted therapy has been developed, showing the first results. This article presents a case of familial X-linked hypophosphatemia.Case Report. Twin girls, premature babies, with low birth weight, having complications during the newborn period. There was low growth, late teething, delayed speech development, and varus deformity of the lower extremities in past medical history. A biochemical blood test showed an increase in the level of alkaline phosphatase, hypophosphatemia, and a decrease in the level of tubular phosphorus reabsorption. The characteristic signs of rickets were recorded on the X-rays. Phosphorous buffer preparations, the active form of vitamin D, were used in the treatment. The analysis of 166 genes responsible for bone pathology was carried out using high-performance parallel sequencing using the target panel “Hereditary diseases of the skeleton”, and a mutation in the PHEX gene was identified. At the age of 4 years and 8 months, a clinical diagnosis was established: X-linked dominant hypophosphatemic rickets. Heterozygous mutation c.1568+1G>A in the PHEX gene. Somatic stunting. Taking into account the O-shaped deformity of the legs, the girls underwent corrective osteotomy on the left tibia. At 6.5 years of age, haemiepiphysiodesis. At the age of 8, pathogenetic treatment with the preparation of monoclonal antibodies to fibroblast growth factor-23 — Burosumab, unregistered in the territory of the Russian Federation, was started. The dynamics of growth and laboratory data on the background of treatment is positive. Conclusion. Timely diagnosis of rare forms of rickets-like diseases is important to improve the quality of life of patients.
Long QT Syndrome Course Associated with New Coronavirus Infection. Clinical Case
Authors: Israfilova Sabina Kh. , Kruchina Tatiana K. , Zhdanova Marina V. , Klimenko Lada О. , Gennadiy Novik A. 
Published: February 2025

Background. Long QT syndrome is hereditary disease, cardiac canalopathy variant, characterized by syncope and high risk of sudden cardiac death due to occurrence of polymorphic ventricular tachycardia (VT), torsade de pointes, and ventricular fibrillation. Acute respiratory viral infection may aggravate clinical course of primary electrical heart diseases. Clinical case description. 14-year-old female patient was transferred to infectious diseases hospital from another hospital with positive PCR test on new coronavirus infection (COVID-19) after loss of consciousness in November 2021. Syncope was first noted at the age of 12, however then did not recur, and their examination and treatment were postponed due to the COVID-19 pandemic. Syncopal states continued during COVID-19. Cardiac examination was performed at infectious diseases hospital: recurrent VT, torsade de pointes, was diagnosed along with QT interval prolongation. The girl's condition has improved after the treatment and selection of antiarrhythmic therapy. 15 days later negative PCR test for SARS-CoV-2 was received, and the patient was transferred to specialized hospital, where the long QT syndrome was confirmed, and dual-chamber cardioverter defibrillator was installed. Conclusion. This clinical case demonstrates that ARVI can aggravate the course of existing primary arrhythmia and emphasizes the crucial role of timely diagnosis and integrated approach to the management of patients with genetically determined cardiac arrhythmias.
The lived experience of drivers with a spinal cord injury: A qualitative inquiry
Authors: Mtetwa Lucia , Classen Sherrilene , Van Niekerk Lana 
Published: February 2025

Introduction: Driving is an instrumental activity of daily living and a facilitator of meaningful participation in society for the majority of the population, including persons with spinal cord injuries. Persons with spinal cord injury may have impaired fitness to drive capabilities. Little is known about perceptions of drivers with spinal cord injury on driving, driver rehabilitation, or return to driving. This study examined the post spinal cord injury driving experiences of drivers and illuminates their rehabilitation and return-to-driving needs within the South African context.Method: This phenomenological study explored personal experiences of fourteen drivers with spinal cord injury, recruited through purposive sampling. Face-to-face, semi-structured interviews were audio recorded and transcribed verbatim. The data analysis was an inductive and iterative process.Results/findings: Six themes represent the study findings: adjusting to physical limitations, safety perceptions and influencing factors, the positive role of driving, contextual features and supports, environmental barriers, and inconsistent provision of rehabilitation services.Conclusions: The findings indicated that occupational therapists ought to consider incorporating driver rehabilitation services and adopt mediation approaches to advocate for persons with spinal cord injury, who want to drive. Plausible practice and research opportunities are discussed for occupational therapists who are interested in driving and spinal cord injury.
Key words: Driving, spinal cord injury, individual perspective, driver rehabilitation.
Problems of Legal Regulation of Savings Certificates
Authors: Lekanova E. E. 
Published: February 2025

Until 2018, the savings certificate was a very attractive and popular banking product. However, after amendments to legislation that led to the abolition of presenting savings certificates, in 2018 credit organizations stopped issuing them. In turn, credit organizations prefer not to issue registered savings certificates, since it is more profitable for them to place funds of the population in deposits that are not certified by savings certificates.The paper assesses the reasons for the prohibition of presenting certificates, establishes their insufficient validity. The paper is relevant since it examines not only the aforementioned legal problem, which requires a legislative reaction, but also he increased need of the Russian economy for «long money» over the past two years. The President of the Russian Federation in his Address to the Federal Assembly in February 2024 proposed to provide special conditions for the placement of irrevocable certificates that would stimulate the population to invest in them at the level of legislation. The paper analyzed the idea of issuing irrevocable certificates and evaluates the conditions for their placement in terms of their attractiveness to the population.
The role of the multidisciplinary team in managing a challenging brittle bone disease with 18 fractures in the modern era: a case report and literature review
Authors: Ramu Sivalakshmi , Das Anamika , Zangmo Rinchen 
Published: February 2025

Osteogenesis imperfecta (OI) is a rare autosomal dominant disorder due to collagen type I biosynthesis defects. It aggressively affects maternal and fetal outcomes in pregnancy. In this case report, we will be discussing the course of pregnancy in an OI patient who has already had 18 fractures since childhood and the challenges faced during the course. A 29-year-old primigravida who was diagnosed with OI since childhood had approximately 18 fractures up to the age of 29. She was under regular antenatal follow-up in our clinic. She was diagnosed with late-onset stage 1 fetal growth restriction at 36 weeks. She was planned for an elective caesarian under a multidisciplinary team at 37 weeks given her short stature, contracted pelvis, and stage 1 fetal growth restriction. She had an uneventful delivery course without any intraoperative and postoperative complications. There was no stress fracture post-delivery, and the newborn developed normally during a 1-year follow-up period. This case highlights the management of OI in pregnancy who had a history of multiple fractures and also emphasizes the need for a tertiary care center in the management of these high-risk patients. A close follow-up of these patients with prenatal genetic testing, regular ultrasound monitoring, and a multi-disciplinary team will aid in an early diagnosis of all the potential underlying complications, thereby leading to favorable maternal and fetal outcomes. A literature review on this topic is also presented.
Parents Understanding of Verbal Abuse in Early Childhood in Sungai Pinang
Authors: Novianti Rahmah , Susanti  , Darwin Effendi  
Published: February 2025

This research was conducted with the aim of knowing parents' understanding of verbal abuse in children with the research location in Sungai Pinang village. The background of this study is that not a few parents who are verbally abusive to their children realise that this attitude will have a negative impact in the future. This study used a descriptive qualitative method involving 7 parents as participants. The findings of this study suggest that most of the parents involved as participants do not understand verbal abuse, as well as the forms and impacts of verbal abuse itself. Thus, this research is expected to provide an overview of parents' understanding of verbal violence in Sungai Pinang village.
Long-term Outcome of Pediatric Arthroscopic Surgery for Avulsion Fracture of the Ligamentum Teres
Authors: Matsushita Yohei , Sugiyama Hajime , Hayama Tetsuo , Sato Ryuichi , Saito Mitsuru 
Published: February 2025

Case:
An 8-year-old boy experienced a traffic accident resulting in avulsion fracture of the ligamentum teres, preventing maintenance of hip joint congruity. Hip arthroscopy was performed to remove the teres and bone fragments. Three months postoperatively, he could walk without a cane. Fourteen years postoperatively, the patient remains well, and his International Hip Outcome Tool (iHOT-12, short version) score improved from 23 points preoperatively to 100.

Conclusion:
We performed arthroscopic hip surgery for managing a ligamentum teres avulsion fracture. This is the first report of the long-term results of arthroscopic treatment of this type of fracture in a pediatric patient.
Geochemical Modeling of Low-Salinity Polymer Flooding for Carbonate Rocks
Authors: Arellano Aaron Guadalupe Tellez , Al-Shalabi Emad W. , Hassan Anas M. , Zeynalli Mursal , Kamal Muhammad S. , Patil Shirish , Hussain Syed M. Shakil 
Published: February 2025

Summary
The demand for global energy has been increasing continuously, and the oil and gas industry is a significant supplier of energy required to meet this demand. However, the industry faces challenges due to regulatory constraints and exploration complexity, which have made it necessary to maximize oil recovery from existing fields. Enhanced oil recovery (EOR) techniques have shown great potential in increasing oil recovery from reservoirs that were produced by conventional primary and secondary recovery methods. Chemical EOR (cEOR) techniques, specifically polymer flooding (PF), have proved valuable in improving the macroscopic sweep efficiency and changing rock and fluid interactions at a reasonable technical and economic cost. To appropriately select a suitable polymer, it is essential to understand the impact of diffusion, polymer adsorption, and geochemical interactions between the polymer, brine, and rock phases.
The main objective of this study is to propose a mechanistic model that captures the physicochemical aspects of polymer flow in porous media through a geochemical perspective using a coupled reservoir flow and geochemical numerical simulator [MATLAB Reservoir Simulation Toolbox (MRST)-IPhreeqc simulator] for applications in carbonate rocks. We developed a mechanistic model using MRST reservoir flow and the IPhreeqc geochemical simulator, with the MRST polymer module modified to model key parameters such as polymer viscosity, adsorption, inaccessible pore volume (IPV), residual resistance factor, hydrolysis, and shear effects. Surface complexation modeling from IPhreeqc was integrated to model Indiana limestone carbonate rocks and introduced a polymer species of sodium acrylamido tertiobutyl sulfonate (ATBS) polymer for interaction within the MRST simulator for low-salinity polymer (LSP) flooding paradigm. The adsorption equilibrium is captured through thermodynamic reactions and flow equations. The updated simulator was validated against experimental tests for carbonate rocks. The results prove the simulator’s effectiveness in modeling the main mechanisms of LSP. This study offers insights into geochemical, reservoir flow, and adsorption in PF. The integration of geochemical factors is crucial for optimizing PF in the Middle East’s harsh carbonate reservoir conditions, enhancing regional oil recovery. Furthermore, the sensitivity analysis demonstrated that polymer concentration significantly impacts calcite dissolution, polymer adsorption, and pH changes in the produced brine, highlighting the critical role of geochemical interactions in optimizing PF strategies.
Adjusting the dosing schedule of eribulin to every 4 weeks (twice, on day 1 and day 8) improved overall survival in patients with human epidermal growth factor receptor 2-negative metastatic and advanced breast cancer
Authors: Mizuno Yutaka , Toyoda Chihiro , Shimizu Yoshimi , Ichikawa Takahiro , Shibata Masahiro 
Published: February 2025

Abstract

Purpose: Eribulin, a non-taxane microtubule inhibitor, is often unsustainable on the standard dosing schedule (days 1 and 8 every 3 weeks, Q3W) owing to severe hematological toxicities. We compared overall survival (OS) between this schedule and a modified one (days 1 and 8 every 4 weeks, Q4W).
Methods: This single-center prospective cohort study compared objective response rate (ORR), progression-free survival (PFS), PFS2, OS, and safety in patients with breast cancer receiving eribulin on either a Q3W dosing schedule (n = 47) or a Q4W dosing schedule (n = 31).
Results: The median PFS was 5.5 months for the Q3W group and 9.0 months for the Q4W group (p = 0.065). The median PFS2 was 8.9 months for Q3W and 19.3 monthsfor Q4W (p= 0.098). In contrast, the median OS was 15.0 months for Q3W and 26.7 months for Q4W (p = 0.040). The median OS was longer in patients with a baseline absolute lymphocyte count (ALC) of ≥1500/μL (p = 0.007) and in the Q4W group (p = 0.001). Multivariable analyses identified baseline ALC and dosing schedule as independent OS predictors (p = 0.022 and p = 0.006). The frequency and severityof hematologic toxicities, including leukopenia and neutropenia, were similar between groups.
Conclusion: Adjusting the eribulin dosing schedule from Q3W to Q4W improved the OS of patients with human epidermal growth factor receptor 2-negative advanced and metastatic breast cancer who showed a neutrophil count of <1500 mm³ on day 1 of the next cycle.
The Devastating Impact of University Closures on theMental Health of Universityof Khartoum Students: A Quantitative Exploration
Authors: Suliman Mona 
Published: February 2025

Abstract

This study examines the impact of university closures on the mental health of students at Sudan’s University of Khartoum, a population uniquely affected by prolonged political instability, economic collapse (250% inflation in 2022), and mass displacement (4 million internally displaced). Using quantitative data from 500 students, regression analysis revealed a strong correlation between prolonged closures and heightened psychological distress. Key findings indicate alarming rates of stress (75%), anxiety (68%), depression (60%), and suicidal ideation (18%), exacerbated by academic disruption, social isolation, and financial strain. The study highlights the urgent need for emergency online counseling, virtual learning platforms, and national policy reforms to safeguard students’ well-being. Despite limitations such as reliance on self-reported data, these findings underscore that Sudan’s recovery hinges on protecting its educated youth.
Genome-wide analyses of Mycobacterium tuberculosis complex isolates reveal insights into circulating lineages and drug resistance mutations in The Gambia
Authors: Tientcheu Leopold , Faal Fatou , Top Naffie , Jobe Olimatou , Colley Sang Marie , Ayorinde Abigail , Mendy Alieu , Sarr-Kuyateh Binta , Donkor Simon , Antonio Martin , de Jong Bouke , Rachow Andrea , Kampmann Beate , Sutherland Jayne S. , Li Hongwei , Blundell Tom , Campino Susana , Kohl Thomas , Dreyer Viola , Niemann Stefan , Pandurangan Arun , Clark Taane , Phelan Jody 
Published: February 2025

Abstract

Tuberculosis (TB), caused by the Mycobacterium tuberculosis complex (MTBC), remains a pressing global health challenge, with the West African region, including The Gambia, experiencing a substantial burden. This study explores the genetic diversity of MTBC strains circulating in The Gambia for nearly two decades (2002–2021) to enhance understanding of drug resistance dynamics and inform targeted diagnostic and treatment strategies. Using whole-genome sequencing (WGS) data from 1,803 TB isolates, we identified the predominance of lineage 4 (L4, 67.2%) and lineage 6 (L6, 26.6%) strains, with L4 showing more significant genetic variability over time. Drug susceptibility analysis of these isolates revealed that 78% (1421 isolates) were drug-susceptible, while 6.5% (119 isolates) exhibited resistance, primarily to isoniazid, rifampicin, and their combination. Additionally, 15.5% (282 isolates) were classified as Other, having potential drug-resistance mutations of uncertain significance by the WHO catalogue. Interestingly, our resistance-associated analysis showed the lineage 6 specific ethambutol uncertain significance (by WHO catalogue) mutation (embC Ala307Thr) more prevalent in The Gambia than in West Africa and globally. Structural analysis showed that first-line drug resistance mutations frequently occur in solvent-inaccessible and conserved regions of proteins, often impacting protein stability and reflecting a balance between resistance, fitness, and evolutionary adaptation. This study highlights the coexistence of globally prevalent and regionally restricted MTBC lineages, underscoring the importance of region-specific TB control measures. Integrating bioinformatic and structural analyses revealed many uncertain significant mutations by the WHO catalogue in The Gambian isolates compared to West Africa and globally. These findings reinforce the necessity of continuous genomic surveillance to address the evolving challenges of TB in high-burden settings like West Africa.
WITHDRAWN: Aortic recoarctation in infants: risk factors and the role of catheter intervention in its management
Authors: Al-Mutairi Mansour B. , Alnajjar Abdulhameed A. , R.Abdelrehim Ayman , Allugmani Mohammad , Morsy Mohamed F. , Khoshhal Saad , Baangood Luna S. , Alahmadti Ahmad , Ishak Abdulaleem , Ibrahim Andreen , Salem Sherif S. 
Published: February 2025

Abstract

The full text of this preprint has been withdrawn by the authors while they make corrections to the work. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.