bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a liquid chromatography assay for the determination of opicapone and BIA 9–1079 in rat matrices
Authors: Gonçalves Daniela , Alves Gilberto , Fortuna Ana , Soares‐da‐Silva Patrício , Falcão Amílcar 
Published: July 2015

AbstractOpicapone is a novel potent, reversible and purely peripheral third generation catechol‐O‐methyltransferase inhibitor, currently under clinical trials as an adjunct to levodopa therapy for Parkinson's disease. To support additional nonclinical pharmacokinetic studies, a novel high‐performance liquid chromatographic method coupled to a diode array detector (HPLC‐DAD) to quantify opicapone and its active metabolite (BIA 9–1079) in rat plasma and tissues (liver and kidney) is herein reported. The analytes were extracted from rat samples through a deproteinization followed by liquid‐liquid extraction. Chromatographic separation was achieved in less than 10 min on a reversed‐phase C18 column, applying a gradient elution program with 0.05 M monosodium phosphate solution (pH 2.45 ± 0.05) and acetonitrile. Calibration curves were linear (r2 ≥ 0.994) within the ranges of 0.04‐6.0 µg/mL for both analytes in plasma, 0.04‐4.0 µg/mL for opicapone in liver and kidney homogenates, and 0.07‐4.0 µg/mL and 0.06‐4.0 µg/mL for BIA 9–1079 in liver and kidney homogenates, respectively. The overall intra‐ and inter‐day accuracy ranged from −12.68% to 7.70% and the imprecision values did not exceed 11.95%. This new HPLC‐DAD assay was also successfully applied to quantify opicapone and BIA 9–1079 in a preliminary pharmacokinetic study. Copyright © 2015 John Wiley & Sons, Ltd.
A de Novo‐Designed Monomeric, Compact Three‐Helix‐Bundle Protein on a Carbohydrate Template
Authors: Malik Leila , Nygaard Jesper , Cristensen Niels J. , Madsen Charlotte S. , Rösner Heike I. , Kragelund Birthe B. , Hoiberg‐Nielsen Rasmus , Streicher Werner W. , Arleth Lise , Thulstrup Peter W. , Jensen Knud J. 
Published: July 2015

AbstractDe novo design and chemical synthesis of proteins and of other artificial structures that mimic them is a central strategy for understanding protein folding and for accessing proteins with new functions. We have previously described carbohydrates that act as templates for the assembly of artificial proteins, so‐called carboproteins. The hypothesis is that the template preorganizes the secondary structure elements and directs the formation of a tertiary structure, thus achieving structural economy in the combination of peptide, linker, and template. We speculate that the structural information from the template could facilitate protein folding. Here we report the design and synthesis of three‐helix‐bundle carboproteins on deoxyhexopyranosides. The carboproteins were analyzed by CD, analytical ultracentrifugation (AUC), small‐angle X‐ray scattering (SAXS), and NMR spectroscopy, and this revealed the formation of the first compact and folded monomeric carboprotein, distinctly different from a molten globule. En route to this carboprotein we observed a clear effect originating from the template on protein folding.
One‐Pot Chemoenzymatic Cascade for Labeling of the Epigenetic Marker 5‐Hydroxymethylcytosine
Authors: Nifker Gil , Levy‐Sakin Michal , Berkov‐Zrihen Yifat , Shahal Tamar , Gabrieli Tslil , Fridman Micha , Ebenstein Yuval 
Published: July 2015

AbstractThe epigenetic DNA modification 5‐hydroxymethylcytosine (5‐hmC) is important for the regulation of gene expression during development and in tumorigenesis. 5‐hmC can be selectively glycosylated by T4 β‐glucosyltransferase (β‐GT); introduction of an azide on the attached sugar provides a chemical handle for isolation or fluorescent tagging of 5‐hmC residues by click chemistry. This approach has not been broadly adopted because of the challenging synthesis and limited commercial availability of the glycosylation substrate, 6‐deoxy‐6‐azido‐α‐D‐glucopyranoside. We report the enzyme‐assisted synthesis of this precursor by the uridylyltransferase from Pasteurella multocida (PmGlmU). We were able to directly label 5‐hmC in genomic DNA by an enzymatic cascade involving successive action of PmGlmU and β‐GT. This is a facile and cost‐effective one‐pot chemoenzymatic methodology for 5‐hmC analysis.
Broadband Light‐Harvesting Molecular Triads with High FRET Efficiency Based on the Coumarin–Rhodamine–BODIPY Platform
Authors: He Longwei , Zhu Sasa , Liu Yong , Xie Yinan , Xu Qiuyan , Wei Haipeng , Lin Weiying 
Published: July 2015

AbstractBroadband capturing and FRET‐based light‐harvesting molecular triads, CRBs, based on the coumarin–rhodamine–BODIPY platform were rationally designed and synthesized. The absorption band of CRBs starts from blue–green to yellow–orange regions (330–610 nm), covering the strong radiation scope of sunlight. The peripheral coumarin and BODIPY chromophore energy could transfer to the central acceptor rhodamine by a one‐step direct way. The energy of the coumarin moiety could also transfer to the BODIPY unit, subsequently transferring to the rhodamine core by two‐step sequential ways. Both the efficiencies of the coumarin moiety and the BODIPY unit to the rhodamine core in CRBs, determined by two different ways, are very high.
EphB1 and EphB2 intracellular domains regulate the formation of the corpus callosum and anterior commissure
Authors: Robichaux Michael A. , Chenaux George , Ho Hsin‐Yi Henry , Soskis Michael J. , Greenberg Michael E. , Henkemeyer Mark , Cowan Christopher W. 
Published: July 2015

ABSTRACTThe two cortical hemispheres of the mammalian forebrain are interconnected by major white matter tracts, including the corpus callosum (CC) and the posterior branch of the anterior commissure (ACp), that bridge the telencephalic midline. We show here that the intracellular signaling domains of the EphB1 and EphB2 receptors are critical for formation of both the ACp and CC. We observe partial and complete agenesis of the corpus callosum, as well as highly penetrant ACp misprojection phenotypes in truncated EphB1/2 mice that lack intracellular signaling domains. Consistent with the roles for these receptors in formation of the CC and ACp, we detect expression of these receptors in multiple brain regions associated with the formation of these forebrain structures. Taken together, our findings suggest that a combination of forward and reverse EphB1/2 receptor‐mediated signaling contribute to ACp and CC axon guidance. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 405–420, 2016
Cortical activation associated with determination of depth order during transparent motion perception: A normalized integrative fMRI–MEG study
Authors: Natsukawa Hiroaki , Kobayashi Tetsuo 
Published: July 2015

AbstractWhen visual patterns drifting in different directions and/or at different speeds are superimposed on the same plane, observers perceive transparent surfaces on planes of different depths. This phenomenon is known as transparent motion perception. In this study, cortical activities were measured using functional magnetic resonance imaging (fMRI) and magnetoencephalography (MEG) to reveal the cortical dynamics associated with determination of depth order during transparent motion perception. In addition, offline eye movement measurements were performed to determine the latencies of the start of both pursuit eye movements and depth attention that are important in determination of the depth order. MEG and fMRI data were analyzed by a normalized integrative fMRI–MEG method that enables reconstruction of time‐varying dipole moments of activated regions from MEG signals. Statistical analysis of fMRI data was performed to identify activated regions. The activated regions were used as spatial constraints for the reconstruction using the integrative fMRI–MEG method. We focused on the period between latencies (216–405 ms) determined by eye movement experiment, which are related to determination of the depth order. The results of integrative analysis revealed that significant neural activities were observed in the visual association area, the human middle temporal area, the intraparietal sulcus, the lateral occipital cortex, and the anterior cingulate cortex between 216 and 405 ms. These results suggest that initial eye movement and accompanying cortical activations during focused duration play an important role in determining the depth order during transparent motion perception. Hum Brain Mapp 36:3922–3934, 2015. © 2015 Wiley Periodicals, Inc.
Benzoyl Peroxide/2‐Vinylpyridine Synergy in RAFT Polymerization: Synthesis of Poly(2‐vinylpyridine) with Low Dispersity at Ambient Temperature
Authors: Luo Juxiang , Li Mingchun , Xin Meihua , Sun Weifu 
Published: July 2015

Redox‐initiated reversible addition fragmentation chain transfer polymerization of 2‐vinylpyridine at room temperature without a conventional reducing agent has been realized in the presence of an oxidizing agent only, i.e., benzoyl peroxide. Well‐defined poly(2‐vinylpyridine) is obtained with a low dispersity (dispersity = Mw/Mn = 1.11) and a conversion efficiency of 41.4% after 24 h at 25 °C. The kinetics, number‐average molecular weight, and dispersities for the polymerization of 2‐vinylpyridine are investigated. The results indicate that the number‐average molecular weight of the poly(2‐vinylpyridine) increases with the monomer conversion while retaining relatively low dispersity (Mw/Mn < 1.20).
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Automatic weather stations and the quality function deployment method
Authors: Begeš G. , Drnovšek J. , Bojkovski J. , Knez J. , Groselj D. , Černač B. , Hudoklin D. 
Published: July 2015

ABSTRACTThe reliability of ground‐based measurements is important in a variety of meteorological areas, such as weather forecasts, air quality and climate. In order to improve this reliability, closer co‐operation with the metrological community has been established. This paper looks at another possible route for improvements, based on carrying out a quality assessment of ground‐based measurements using advanced quality techniques. The quality function deployment (QFD) method was adapted so as to be able to analyse the results of a survey that was conducted among meteorological community. They were asked to analyse the key performance characteristics of typical automatic weather stations (AWSs) systematically with respect to the World Meteorological Organization (WMO) classifications and requirements. The QFD is a well‐known quality tool that connects customer expectations with technical functionalities or engineering characteristics. It makes it possible to prioritize the needs and quantify the results of the assessment. The answers from the survey's questionnaire were used as the input data for the QFD matrix. The findings reveal that the closest correlations are between the customer expectations or attributes and the engineering characteristics, e.g., the validation of the AWSs and the measurement methods, the regular calibration of sensors, and the implementation of automated and manual controls. From the second level of the QFD matrix, it was found that the most important technical solution serving the previously mentioned needs is related to having control over the development and upgrading of the AWSs' software and in terms of an automatic check and the transfer of data to the central database.
Mit Röntgenabsorption chemische Prozesse verstehen
Authors: Bauer Matthias 
Published: July 2015

AbstractUm nachhaltige chemische Prozesse zu entwickeln, ist ein Verständnis solcher Reaktionen auf atomarer Ebene nötig. Die Absorptionsspektroskopie mit harter Röntgenstrahlung klärt unabhängig vom Aggregatzustand der Probe elementspezifisch die Details.
Verhaltenes Wachstum
Authors: Bulmahn Maren 
Published: July 2015

AbstractDas Jahr 2014 brachte erhöhte Umsätze, bei manchen Unternehmen Rekordergebnisse und allgemein den Trend zu stärkerer Spezialisierung. Dies zeigen etwa Umstrukturierungen und Patentanmeldungen.
Chiralität und Laserionisations‐ Massenspektrometrie
Authors: Reusch Nicola , Weitzel Karl‐Michael 
Published: July 2015

AbstractEnantiomere lassen sich mit ultrakurzen Laserpulsen unterscheiden. Die Kopplung der Chiralitätsanalyse in einer gepulsten Laserionisation mit massenspektrometrischer Analyse ermöglicht es zudem, mehrere Substanzen in einem Gemisch simultan zu untersuchen.
Mehr Umsatz mit Geräten
Authors: Zbikowski Frauke 
Published: July 2015

AbstractEin gutes Ergebnis erzielten die deutschen Hersteller von Labor‐ und Analysengeräten im Jahr 2014. Die weltweit Größten der Branche sind nach wie vor vier US‐Unternehmen. Von diesen meldete nur Agilent einen Umsatzrückgang, bleibt aber die Nummer zwei auf dem Markt.
1,1 Millionen Euro für moderne Praktika
Authors: Schirmer Johanna 
Published: July 2015

Abstract17 Chemiefachbereiche an 15 Standorten erhielten im Jahr 2014 Zuschüsse vom Fonds der chemischen Industrie, um ihre Praktika zu modernisieren. Die meisten kauften damit analytische Geräte, darunter ein Western‐Blot‐Analysengerät und ein Elektronenspinresonanzspektrometer.
Vitamin D, inflammation, and relations to insulin resistance in premenopausal women with morbid obesity
Authors: Nguyen Van T. , Li Xianjun , Elli Enrique F. , Ayloo Subhashini M. , Castellanos Karla J. , Fantuzzi Giamila , Freels Sally , Braunschweig Carol L. 
Published: July 2015

ObjectiveIn this study, the associations between vitamin D, insulin sensitivity, and inflammation and their relationships with adipose tissue expression of vitamin D receptor (VDR) and inflammatory markers in women with morbid obesity were determined.MethodsAn oral glucose tolerance test prior to surgery was completed by healthy premenopausal women (n = 76) seeking bariatric surgery. Abdominal subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were collected during surgery.ResultsApproximately, 70% of our subjects were vitamin D sufficient or optimal, and 80% had normal glucose tolerance. No significant association between serum 25‐hydroxyvitamin D [25(OH)D] with circulating inflammatory markers or insulin sensitivity was identified. In subjects with waist circumference of <139 cm (n = 42), log25(OH)D positively predicted VAT logIL‐6 mRNA expression (P = 0.003). LogVDR expression was positively correlated with the expression of inflammatory markers in both SAT (logIL‐1β mRNA: r = 0.95, P < 0.0001; logTNF mRNA: r = 0.82, P < 0.0001) and VAT (logIL‐1β mRNA: r = 0.89, P < 0.0001; logTNF mRNA: r = 0.75, P < 0.0001). VAT logVDR expression positively predicted logHOMA‐IR in non‐African American subjects (P = 0.05).ConclusionsThe beneficial effects of vitamin D on inflammation and insulin sensitivity were not supported by our findings. VDR does not appear to possess a protective effect in adipose tissue.
C‐peptide modifies leptin and visfatin secretion in human adipose tissue
Authors: Garcia‐Serrano Sara , Gutiérrez‐Repiso Carolina , Gonzalo Montserrat , Garcia‐Arnes Juan , Valdes Sergio , Soriguer Federico , Perez‐Valero Vidal , Alaminos‐Castillo Miguel A. , Francisco Cobos‐Bravo Juan , Moreno‐Ruiz Francisco J. , Rodriguez‐Cañete Alberto , Rodríguez‐Pacheco Francisca , Garcia‐Escobar Eva , García‐Fuentes Eduardo 
Published: July 2015

ObjectiveThe effects of C‐peptide on adipose tissue, an organ involved in the development of obesity and insulin resistance, are not yet well known. The aim of this study was to determine whether C‐peptide could be involved in the regulation of the adipocytokine synthesis in human visceral adipose tissue.MethodsThe association between C‐peptide and different serum adipocytokines, with an intravenous glucose tolerance test (IVGTT), and in an in vitro study in subjects without obesity and in subjects with morbid obesity were analyzed.ResultsIn different multiple regression analysis models, C‐peptide and C‐peptide increase above basal levels during total IVGTT and between 0 and 10 min were associated positively with leptin and negatively with visfatin. Rhodamine‐labeled C‐peptide binds to human adipocytes, and this binding was blocked with excess of unlabeled C‐peptide. Exposure of human visceral explants and adipocytes from subjects with morbid obesity to C‐peptide at 1 and 10 nM induced a significant increase in leptin and a decrease in visfatin secretion. In subjects without obesity, these C‐peptide effects were found mainly at 10 nM. These effects can be inhibited by phosphatidylinositol 3‐kinase (PI3K) or protein kinase B (PKB) inhibitors.ConclusionsC‐peptide may be involved in the regulation of leptin and visfatin secretion, molecules intimately involved in energy homeostasis processes, through PI3K or PKB pathways.
Preface: physica status solidi (c) 7/2015
Authors: Alexandrov Sergei , Hoffmann Patrik , Knez Mato , Vahlas Constantin 
Published: July 2015

AbstractDear participants of EuroCVD20,with pleasure we address you some words about the present conference proceedings of EuroCVD20.We faced a big challenge organizing a conference to which the full paper peer reviewed contributions will be available already with the launch of the conference!Clearly, the advantage of this procedure is the highest quality of the conference proceedings, namely peer reviewed scientific publications. Already before listening to the presentation or discussing the poster with the author, the participant of the conference will get exposed to the scientific topic, which makes him/her able to better understand the background. This opportunity opens the doors for scientific exchange with the author at its best. The educational aspects of top notch scientific discussions are based on understanding of the topics and competence in communicating the questions and answers. As time slots for oral or poster presentations at conferences are very limited the message transfer has to be well prepared between the peers, i.e. the author of the contribution and the reader of the manuscript.We are aware that some aspects speak against this procedure. The most important argument is that the latest results will not be reported in the paper. We, however, trust that none of the conference participants will be unhappy seeing latest results of a research work in the conference presentation only. Moreover, a scientifically founded discussion might even trigger optimization of the latest results and publication in a follow‐up communication.Therefore we are happy to put at disposition to all participants of the EuroCVD20 conference 49 peer reviewed full papers that have been published either in pss(a) or pss(c).We, the co‐editors of the EuroCVD20 conference proceedings, wish all readers of the collected papers insight, understanding, fun and pride. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Towards a metallic quasi‐d9 system without copper: AgO at high pressure
Authors: Włodarska Izabela , Derzsi Mariana , Grochala Wojciech 
Published: July 2015

AgO is a prototypical mixed‐valence compound, with markedly different coordination environment of dumbbell Ag(1+) and low‐spin square‐planar Ag(3+) which render it a narrow band gap semiconductor. The hybrid HSE06 functional reproduces fairly well the band gap of its P 21/c form at ambient conditions (ΔEexp = 1.0–1.1 eV, ΔEtheor = 0.94 eV) and suggest progressive band gap decrease with external pressure with metallization at 50 GPa via band overlap. Dynamic (phonon) instability appears at the onset of metallization leading to a structural phase transition to a more stable but still metallic form. The density of electronic states at the Fermi level of the polymorph is small and a pseudo‐gap at the Fermi level is preserved.
Illustration of the predicted P21/c → transformation.magnified imageIllustration of the predicted P21/c → transformation.(© 2015 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)
Applying multiple proteases to direct digestion of hundred‐scale cell samples for proteome analysis
Authors: Chen Qi , Yan Guoquan , Zhang Xiangmin 
Published: July 2015

RationaleAnalyzing the proteome on the scale of only several hundred cells with mass spectrometry has great significance for applications with limited sample amounts. We applied multiple proteases to the direct digestion of cells and compared the identified proteins both qualitatively and quantitatively.MethodsThree hundred cells were directly digested by trypsin, chymotrypsin, or the combination of trypsin and chymotrypsin. The peptides were identified using a LTQ‐Orbitrap XL, and data were analyzed using MaxQuant software.ResultsDifferent proteases produced different identified protein numbers. Trypsin proved to be the best choice for generating the largest protein number, while other proteases complemented the identification results of trypsin by increasing protein sequence coverage. Concerning the quantitative perspective, using trypsin would produce the biggest number of proteins quantifiable by intensity‐based absolute quantification (iBAQ).ConclusionsWhen hundred‐scale cell samples are analyzed, an optimum choice of proteases should be made to realize different analytical objectives. Copyright © 2015 John Wiley & Sons, Ltd.
Modeling Success: Using Preenrollment Data to Identify Academically At‐Risk Students
Authors: Gansemer‐Topf Ann M. , Compton Jonathan , Wohlgemuth Darin , Forbes Greg , Ralston Ekaterina 
Published: July 2015

AbstractImproving student success and degree completion is one of the core principles of strategic enrollment management. To address this principle, institutional data were used to develop a statistical model to identify academically at‐risk students. The model employs multiple linear regression techniques to predict students at risk of earning below a 2.0 grade point average (GPA) in their first semester of college. Data analysis from student cohorts starting in the Fall 2007 through Fall 2009 (N = 11,644) identified two groups of students—one predicted to earn less than a 2.0 and the other predicted to earn a 2.0 or higher. The first semester college GPA and retention rates of both groups of students were tracked to examine the accuracy of the model in predicting student success and subsequent retention rates. Multi‐year analyses illustrates that the model can be used to identify students who are at risk of earning less than a 2.0 GPA. Additional analysis demonstrates there is a relationship between predicted and actual first semester GPA and retention rates. Since the data used to develop the model are commonly available at most institutions, this study provides a practical approach for the SEM research professional to identify potentially academically at‐risk students, which subsequently can be used to assist students and improve student success and degree completion.