bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal variation in the deposition of different types of collagen within a porous biomaterial implant
Authors: White Jacinta F. , Werkmeister Jerome A. , Bisucci Teresa , Darby Ian A. , Ramshaw John A. M. 
Published: November 2013

AbstractThe deposition of new collagen in association with a medical implant has been studied using expanded polytetrafluoroethylene vascular replacement samples implanted subcutaneously in sheep, for up to 28 days. New type I collagen mRNA synthesis was followed by in situ hybridization, while the accumulation of new collagen types III, V, VI, XII, and XIV was followed by immunohistochemistry. All the collagen detected in the pores of the implant were newly deposited at various times after implantation and were not due to any pre‐existing dermal collagen that may have been present around the implant. Collagen deposition was seen initially surrounding the implant and, with time, was seen to infiltrate within its pores. In situ hybridization showed that the majority of infiltrating cells had switched on mRNA that coded for type I collagen production. Histology showed that cellular infiltration increased with time, accompanied by increasing collagen deposition. The deposition of different collagen types happened at different rates. The type V and VI collagens preceded the major interstitial collagens in the newly deposited tissue, although at longer time points, detection of type V collagen appeared to decrease. After disruption of the interstitial collagens with enzyme, the “masked” type V collagen was clearly still visible by immunohistochemistry. Little type XII collagen could be seen within the porous mesh, although it was seen in the surrounding tissues. By contrast, type XIV was seen throughout the porous structure of the implanted mesh, with less being visible outside the material where type XII was more abundant. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3550–3555, 2014.
Indigenously produced polyhydroxyalkanoate based co‐polymer as cellular supportive biomaterial
Authors: Shabna A. , Saranya V. , Malathi J. , Shenbagarathai R. , Madhavan H. N. 
Published: November 2013

AbstractThe focus of this study was to evaluate the growth of the cells on a scaffold based on novel polyhydroxyalkanoate (PHA) (Polyhydroxy propionate copoly hydroxy ocatadecanoate copolymer), derived from a mutant strain of Pseudomonas sp. Naive PHA was also blended with several biodegradable polymeric materials (PEG, PLA, and MMT) to improve the scaffold properties. Protein adsorption study was done to evaluate the capability of scaffolds for cellular interaction. PHA:PEG blended scaffold showed better adsorption than others. 3T3 fibroblast cultures on various polymers were equally viable when compared with control culture except for the blend PHA:MMT by CCK 8 kit. MTT assay, performed with the continuous cultures HeLa, HEp‐2, Vero, and McCoy on the polymer blends, supported the above finding. Among the blends PHA:PEG showed increased viability and was selected for further studies. Cell proliferation assay with colorimetric BrdU ELISA kit showed increase in cell proliferation over the matrix PHA:PEG than that of control. There were no observable morphological changes of continuous cells grown over matrix PHA:PEG when observed by phase contrast microscopy. HEp‐2 cells were enclosed within the matrix when analyzed by SEM. The current study states that the scaffold prepared by using the indigenous PHA in combination with PEG supports cell growth better than the conventional plastic surface. PHA:PEG would be a promising material for tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3470–3476, 2014.
Chondrogenic differentiation of mesenchymal stem cells in a hydrogel system based on an enzymatically crosslinked tyramine derivative of hyaluronan
Authors: Dvořáková Jana , Kučera Lukáš , Kučera Jan , Švík Karol , Foglarová Marcela , Muthný Tomáš , Pravda Martin , Němcová Miroslava , Velebný Vladimír , Kubala Lukáš 
Published: November 2013

AbstractHyaluronan‐based tissue substitutes are promising materials in cartilage reconstruction surgery. Herein, the chondrogenesis of human mesenchymal stem cells (MSC) in a hydrogel based on a tyramine derivative of hyaluronan crosslinked by hydrogen peroxidase (HA‐TA) was evaluated. Human MSC seeded in the scaffold were incubated in standard chondrogenic medium and medium enriched with bone morphogenetic protein‐6 (BMP6). Cell viability, the gene expression of selected markers (collagen type II, aggrecan, SOX9, collagen type X, and osteopontin), and the histological characteristics were examined during three weeks of in vitro cultivation. The tissue reaction of both unseeded and MSC seeded HA‐TA scaffolds were tested in vivo after subcutaneous application in rats for 12 weeks. The data showed that cells resisted the process of crosslinking and remained viable for the whole time while exhibiting changes in cell organization. Human MSC cultivated in HA‐TA hydrogel expressed genes of both chondrogenic and osteogenic differentiation and the addition of BMP6 revealed a tendency to potentiate both processes. Histological analysis of HA‐TA in vivo implants did not reveal a chronic inflammatory reaction. In both cases, in vivo HA‐TA implants were continuously degraded and MSC‐seeded hydrogels tended to form clusters similar to in vitro samples. In conclusion, MSC chondrogenic differentiation may proceed in a HA‐TA scaffold that is biocompatible. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3523–3530, 2014.
Search for varicella zoster virus DNA in saliva of healthy individuals aged 20–59 years
Authors: Birlea Marius , Cohrs Randall J. , Bos Nathan , Mehta Satish K. , Pierson Duane L. , Gilden Don 
Published: November 2013

AbstractAll neurological and ocular complications of varicella zoster virus (VZV) reactivation can occur without rash. Virological verification requires detection of VZV DNA or anti‐VZV IgG antibody in cerebrospinal fluid (CSF), or anti‐VZV IgM antibody in serum or CSF. If VZV were readily detected in other tissue in patients with neurological disease without rash and found to correlate with tests listed above, more invasive tests such as lumbar puncture might be obviated. Saliva is a potential source of VZV DNA. To study the potential diagnostic value of detecting VZV DNA in saliva from patients with neurological disease, saliva of healthy adults was searched for VZV DNA. A single saliva sample obtained by passive drool was centrifuged at 16,000g for 20 min. DNA was extracted from the supernatant and cell pellet and examined in triplicate for VZV DNA by real time PCR. A single random saliva sample from 80 healthy men and women aged 20–59 years revealed no VZV DNA (Table ), but was uniformly positive for cell (GAPdH) DNA. Because VZV DNA was not found in a random saliva sample from 80 individuals 20–59‐year‐old, a VZV‐positive sample during neurologic disease may have potential significance. Further studies will determine whether VZV DNA in saliva correlates with VZV DNA or anti‐VZV antibody in CSF in patients with neurological disease. J. Med. Virol. 86:360–362, 2014. © 2013 Wiley Periodicals, Inc.
Polymorphisms in human leukocyte antigens, human platelet antigens, and cytokine genes in hantavirus cardiopulmonary syndrome patients from Ribeirão Preto, Brazil
Authors: Borges Alessandra Abel , Donadi Eduardo Antonio , Campos Gelse Mazzoni , de Figueiredo Glauciane Garcia , Saggioro Fabiano Pinto , Badra Soraya Jabur , Deghaide Neifi Hassan Saloum , Figueiredo Luiz Tadeu Moraes 
Published: November 2013

AbstractHantavirus cardiopulmonary syndrome is a severe human disease associated with hantavirus infection. The clinical course of illness varies greatly among individuals, possibly due to viral and immunological elements and the influence of host genetic factors on clinical outcome. As the magnitude of immune activation has been associated with disease severity, polymorphisms in genes involved in the immune response that may affect the development of this syndrome were investigated. Polymorphisms in the TGF‐β, IL‐10, IL‐6, and IFN‐γ genes, human leukocyte antigens (HLA), and human platelet alloantigens (HPA) genes were investigated in 122 patients with Araraquara virus infection from Ribeirão Preto, Brazil. Patients were divided into two groups: hantavirus cardiopulmonary syndrome (HCPS group; n = 26) and hantavirus seropositive only (n = 96). The frequencies of HLA alleles, cytokines and platelet antigen genotypes were evaluated in both groups and compared to a control group. The data demonstrated no significant influence of the HLA alleles, HPA, IL‐6, and IL‐10 genotypes on susceptibility to hantavirus infection. However, the hantavirus seropositive group presented a significantly higher frequency of a polymorphism associated with a high IFN‐γ production than the HCPS group. In addition, a genotype associated with high TGF‐β production was found more frequently in individuals infected with hantavirus than in the control group. This phenotype was associated with a less intense thrombocytopenia in the HCPS group and may be protective against the most severe form of hantavirus disease. More studies are required to quantify further the influence of the high TGF‐β producer phenotype on the outcome of hantavirus infection. J. Med. Virol. 86:1962–1970, 2014. © 2013 Wiley Periodicals, Inc.
Association of a single nucleotide polymorphism in HOXB9 with developmental dysplasia of the hip: A case‐control study
Authors: Hao Zheng , Dai Jin , Shi Dongquan , Xu Zhihong , Chen Dongyang , Zhao Baocheng , Teng Huajian , Jiang Qing 
Published: November 2013

ABSTRACTDevelopmental dysplasia of the hip (DDH) is one of the most common skeletal disorders. It comprises a spectrum of abnormalities, including shallow acetabulum and decreased coverage of the femoral head. Genetic component plays a considerable role in the aetiology of DDH. HOXB9 may be involved in the aetiology and pathogenesis of DDH, as it plays an important role in the development of the limbs. Our objective was to evaluate whether single nucleotide polymorphisms (SNPs) of HOXB9 (rs2303486 and rs8844) were associated with DDH in Chinese population. The HOXB9 tag SNPs were genotyped in 460 DDH cases and 562 control subjects by Taqman assay, and their association was examined. rs8844 was not associated with DDH. rs2303486 was associated with DDH in the dominant genetic model (p = 0.037; odds ratio (OR) = 1.32; 95% confidence interval (CI) = 1.02–1.71). After stratification by sex, significant association of the dominant genetic model still existed in the female subjects (p = 0.015; OR = 1.46; 95% CI = 1.08–1.98), but not in the male subjects. After stratification by severity, we discovered an association with hip dislocation in the dominant model (p = 0.042; OR = 1.35; 95% CI = 1.01–1.80), but not with subluxation or instability. HOXB9 is associated with DDH in Chinese. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:179–182, 2014.
Topological insulator as an optical modulator for pulsed solid‐state lasers
Authors: Yu Haohai , Zhang Han , Wang Yicheng , Zhao Chujun , Wang Baolin , Wen Shuangchun , Zhang Huaijin , Wang Jiyang 
Published: November 2013

AbstractTopological insulators are states of quantum matter that have narrow topological nontrivial energy gaps and a large third‐order nonlinear optical response. The optical absorption of topological insulators can become saturated under strong excitation. In this work, with Bi2Se3 as an example, it was demonstrated that a topological insulator can modulate the operation of a bulk solid‐state laser by taking advantage of its saturable absorption. The result suggests that topological insulators are potentially attractive as broadband pulsed modulators for the generation of short and ultrashort pulses in bulk solid‐state lasers, in addition to other promising applications in physics and computing.
Bi‐directional organic light‐emitting diodes with nanoparticle‐enhanced light outcoupling
Authors: Chang Hong‐Wei , Lee Jonghee , Koh Tae‐Wook , Hofmann Simone , Lüssem Björn , Yoo Seunghyup , Wu Chung‐Chih , Leo Karl , Gather Malte C. 
Published: November 2013

AbstractAn effective method is presented for enhancing the outcoupling efficiency of translucent/bi‐directional organic light‐emitting diodes (TL/BD‐OLEDs) with a bottom indium tin oxide (ITO) anode and a top cathode comprised of a thin Ag layer covered with an organic capping layer. Upon insertion of a nanoparticle (NP)‐based scattering layer (NPSL) between the substrate and the ITO anode, the TL/BD‐OLEDs exhibit significantly enhanced external quantum efficiency (EQE) in both emission directions. Furthermore, the NPSL improves the color stability of the TL/BD‐OLEDs over a wide range of viewing angles. Simulations based on geometrical and statistical optics are performed to elucidate the mechanism by which the efficiency is enhanced and to establish strategies for further optimization. Simulations performed on the scattering layers with varying NP volume percentage reveal that the bottom‐side emission is governed by competition between waveguide‐mode extraction and backward scattering by NPs in the film, while the top‐side emission is largely dominated by the latter. Optimized bi‐directional OLEDs achieve a 1.64‐fold enhanced EQE compared to reference devices without NPSL.
Five‐cycle pulses near λ = 3 μm produced in a subharmonic optical parametric oscillator via fine dispersion management
Authors: Haakestad Magnus W. , Marandi Alireza , Leindecker Nick , Vodopyanov Konstantin L. 
Published: November 2013

AbstractFive‐cycle (50 fs) mid‐IR pulses at 80‐MHz repetition rate are produced using a degenerate (subharmonic) optical parametric oscillator (OPO), synchronously pumped by an ultrafast 1560‐nm fiber laser. The effects of cavity dispersion and the length of a periodically poled lithium niobate (PPLN) gain element on the output spectrum and pulse duration are investigated by taking advantage of a very broad (∼ 1000 cm−1) gain bandwidth near the 3.1‐μm OPO degeneracy point. A new method of assessing the total OPO group delay dispersion across its entire spectrum is proposed, based on measuring spectral signatures of trace amounts of molecular gases injected into the OPO cavity.
Prediction of ProteinProtein Interaction Pocket Using L‐Shaped PLS Approach and Its Visualizations by Generative Topographic Mapping
Authors: Hasegawa Kiyoshi , Funatsu Kimito 
Published: November 2013

AbstractProteinprotein interaction (PPI) pockets in a hostguest protein system were predicted using an L‐shaped partial least squares (LPLS) method. LPLS is an extension of standard PLS regression, where, in addition to response vector y and regressor matrix X, an extra data matrix Z is constructed which summarizes the background information on X. The regressor matrix X is a similarity matrix of Tanimoto coefficients of the paired fingerprints of pockets, while the background information Z constitutes eleven physico‐chemical and geometrical parameters for describing a pocket. The Boolean response vector y specifies whether each pocket is PPI or non‐PPI (indicated by 1 and 0, respectively). Constructing two LPLS models, we successfully predicted the PPI pockets of two protein clusters. Clusters 1 and 2 comprised the X‐ray crystal structures of protein‐peptide complexes and protein‐protein complexes, respectively. From the loading plots derived from each model, we could speculate the geometrical constraints of the PPI pockets. These two models are exclusively unique and it was validated by the cross‐prediction simulations. The PPI pockets of cluster 1 were projected onto 2D maps by generative topographic mapping (GTM) and the molecular lipophilic potentials (MLP). Among three examples, the MLP distributions were highly similar because the specimens shared the same p53 guest peptides. Contribution to the Autumn School of Chemoinformatics in Nara, Japan, November 27–28, 2013
Funktionelle Lipidmembranen in der Chiptechnik
Authors: Steinem Claudia 
Published: November 2013

AbstractJede Substanz, die das Innere einer Zelle erreichen soll, muss eine Barriere überwinden. Diese ist nur wenige Nanometer dick, aber aus tausenden von verschiedenen Molekülen zusammengesetzt: die Membran. Wie funktioniert das komplexe Zusammenspiel ihrer Komponenten?
Alles aus einer Hand oder von allem das Beste?
Authors: Landgraf Evelyn 
Published: November 2013

AbstractDie heterogene Planungswelt der Prozessindustrie fordert heterogene Lösungen: Integrated Engineering mit nur einem System geht an der Realität vorbei. Revisionssichere Schnittstellensoftware verbindet unterschiedliche Softwaresysteme.
Amine in Getränken und Fleisch
Authors: Giegold Sascha 
Published: November 2013

AbstractDer Gehalt an biogenen Aminen wie Histamin, Cadaverin oder Tyramin in einem Lebensmittel liefert Hinweise darauf, ob es verdorben ist. Automatisierte Vorsäulenderivatisierung beschleunigt die flüssigchromatographische Bestimmung dieser toxischen Substanzen.
Conjugated block copolymers via functionalized initiators and click chemistry
Authors: Smith Kendall A. , Pickel Deanna L. , Yager Kevin , Kisslinger Kim , Verduzco Rafael 
Published: November 2013

ABSTRACTConjugated block copolymers are potentially useful for organic electronic applications and the study of interfacial charge and energy transfer processes; yet few synthetic methods are available to prepare polymers with well‐defined conjugated blocks. Here, we report the synthesis and thin film morphology of a series of conjugated poly(3‐hexylthiophene)‐block‐poly(9,9‐dioctylfluorene) (P3HT‐b‐PF) and poly(3‐dodecylthiophene)‐block‐poly(9,9‐dioctylfluorene) (P3DDT‐b‐PF) block copolymers prepared by functional external initiators and click chemistry. Functional group control is quantified by proton nuclear magnetic resonance spectroscopy, size‐exclusion chromatography, and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. The thin film morphology of the resulting all‐conjugated block copolymers is analyzed by a combination of grazing‐incidence X‐ray scattering, atomic force microscopy, and transmission electron microscopy. Crystallization of the P3HT or P3DDT blocks is present in thin films for all materials studied, and P3DDT‐b‐PF films exhibit significant PF/P3DDT co‐crystallization. Processing conditions are found to impact thin film crystallinity and orientation of the π–π stacking direction of polymer crystallites. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 154–163
Navigation‐aided visualization of lumbosacral nerves for anterior sacroiliac plate fixation: a case report
Authors: Takao Masaki , Nishii Takashi , Sakai Takashi , Sugano Nobuhiko 
Published: November 2013

AbstractBackgroundAnterior sacroiliac joint plate fixation for unstable pelvic ring fractures avoids soft tissue problems in the buttocks; however, the lumbosacral nerves lie in close proximity to the sacroiliac joint and may be injured during the procedure.MethodsA 49 year‐old woman with a type C pelvic ring fracture was treated with an anterior sacroiliac plate using a computed tomography (CT)–three‐dimensional (3D)‐fluoroscopy matching navigation system, which visualized the lumbosacral nerves as well as the iliac and sacral bones. We used a flat panel detector 3D C‐arm, which made it possible to superimpose our preoperative CT‐based plan on the intra‐operative 3D‐fluoroscopic images.ResultsNo postoperative complications were noted.ConclusionsIntra‐operative lumbosacral nerve visualization using computer navigation was useful to recognize the 'at‐risk' area for nerve injury during anterior sacroiliac plate fixation. Copyright © 2013 John Wiley & Sons, Ltd.
The Neurobiological Basis of the Distress Thermometer: A PET Study in Cancer Patients
Authors: Castelli L. , Tonello D. , D'Agata F. , Caroppo P. , Baudino B. , Zotta M. , Cauda S. , Pinessi L. , Mortara P. , Grassi L. , Bisi G. , Torta R. 
Published: November 2013

AbstractThe objective of this study was to investigate the possible associations between the Distress Thermometer (DT) scores and the brain metabolism of structures involved in stress response. Twenty‐one cancer patients were assessed using the DT, Problem Checklist and Hospital Anxiety and Depression Scale (HADS). The psychological measures were correlated with [18 F]PET‐FDG brain glucose metabolism. Multiple and linear regression and binary logistic regression were run to analyse data.The DT and HADS scores illustrated that 48% of patients were distressed, 19% were depressed and48% were anxious. Results showed that some subcortical areas activity, such as part of midbrain and of hypothalamus, was correlated with the DT scores. The Problem Checklist scores correlated with the activity of the same areas and included more regions in the limbic forebrain and brainstem.Compared with the DT and Problem Checklist, HADS‐Depression scores showed a more extensive pattern of correlation with brain activity, including limbic and cortical areas.The results highlighted that the DT scores correlated with the activity of brain areas typically involved in stress response. Indeed, hypothalamus metabolism was found to be the best predictor of distressed patients. Copyright © 2013 John Wiley & Sons, Ltd.
Robustness‐based performance assessment of a prestressed concrete bridge
Authors: Podroužek Jan , Strauss Alfred , Bergmeister Konrad 
Published: November 2013

AbstractLife‐cycle civil engineering addresses, among other things, the growing number of deteriorating bridges and the associated economic challenges. As a consequence, government bodies, infrastructure and bridge owners as well as industry request objective and rational performance indicators for classification and intervention planning in structural engineering. This paper focuses on a methodology for analysing the damage‐based robustness margins of bridge systems under traffic loading. In particular, a series of emergent deterioration‐based damage scenarios are compared with the actual or virgin state in terms of loadbearing capacity and serviceability. Non‐linear finite element analysis based on a detailed 3D model has a high potential for capturing the available bridge capacity for different degradation phenomena and levels, serving as an input for further reliability‐based performance indicators. Notwithstanding, costs associated with fully probabilistic assessment measures are still prohibitive despite technological advances and new methods of reducing the sample size in Monte Carlo computations. In addition, considering the large uncertainties and imprecision involved, it is imperative that probabilistic schemes are preferred over deterministic assessments.The objective of this article is to present strategies for robustness‐based performance assessment using non‐linear modelling and to discuss relevant reliability‐based quantities and performance indicators in relation to structural damage using the example of specific degradation events in an existing prestressed box girder bridge. Furthermore, some strategies are developed on the basis of the new approach for general complex engineering structures.