bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring oxidative stress during chronic obstructive pulmonary disease exacerbations using malondialdehyde
Authors: Antus Balazs , Harnasi Gabriella , Drozdovszky Orsolya , Barta Imre 
Published: July 2013

AbstractBackground and objectiveOxidative stress plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). In this longitudinal study changes in the level of malondialdehyde (MDA), an end product of polyunsaturated fatty acid peroxidation, were investigated in the airways of patients with acute exacerbation of COPD (AECOPD).MethodsLevels of MDA were measured in sputum and exhaled breath condensate (EBC) of 34 COPD patients at the time of hospital admission due to an acute exacerbation of the disease, and again following treatment at the time of hospital discharge. MDA was also assessed in 21 stable patients with COPD and 20 healthy controls. Measurements were performed using high‐performance liquid chromatography.ResultsSputum MDA levels were significantly increased in AECOPD (220.0 ± 17.5 nmol/L) compared with stable disease (144.6 ± 14.3 nmol/L, P < 0.01) and healthy controls (85.9 ± 11.3 nmol/L, P < 0.001). MDA levels decreased after treatment (190.7 ± 16.3 nmol/L, P < 0.05). In contrast to sputum, EBC MDA levels were comparable between controls, stable COPD patients and AECOPD patients (73.1 ± 5.1 nmol/L, 96.1 ± 11.6 nmol/L and 93.3 ± 7.6 nmol/L, P = NS). Measurement of MDA had good repeatability in both sputum and EBC, but the between‐day variability was considerably higher in EBC. Sputum induction did not influence MDA levels.ConclusionsMDA in sputum, but not in EBC, appears to be a useful marker for monitoring exacerbation‐associated oxidative stress in AECOPD.
Advances in Patient Adherence to Medical Treatment Regimens: The Emerging Role of Technology in Adherence Monitoring and Management
Authors: Bryant Howren M. , Van Liew Julia R. , Christensen Alan J. 
Published: July 2013

AbstractAdherence is a term used to describe the extent to which an individual's behavior coincides with health‐related instructions or recommendations given by a healthcare provider in the context of a specific disease or disorder. Despite significant advances in biomedical science related to the treatment of disease, the problem of nonadherence remains pervasive and may be aptly described as a global public health concern. The recent proliferation of telemedicine coupled with the ubiquity of personal computers, mobile phones, and other wireless communication devices have, in part, led to the development and implementation of a variety of technology‐based methods used to assess, monitor, and manage adherence. As the chronic illness burden continues to grow, these methods may offer solutions to some of the limitations of traditional means particularly for conditions requiring complex self‐management and lifestyle adjustment. In this article, we briefly review recent advances in adherence monitoring and management including m‐health, home telemonitoring systems, web‐based support (i.e., self‐management websites and virtual support groups), and patient portals and personal health records before concluding with a brief account of some exciting new technologies under development in the context of patient adherence.
The Sensitivity to Mean Intentions (SeMI) Model: Basic Assumptions, Recent Findings, and Potential Avenues for Future Research
Authors: Gollwitzer Mario , Rothmund Tobias , Süssenbach Philipp 
Published: July 2013

AbstractA large body of research has demonstrated that people who are habitually sensitive towards victimization tend to behave uncooperatively and immorally in socially uncertain situations. The “Sensitivity to Mean Intentions” (SeMI) model (Gollwitzer & Rothmund 2009) aims at describing social‐cognitive mechanisms that underlie and explain this effect. The model posits that in socially uncertain situations, victim‐sensitive individuals are asymmetrically sensitive to cues of untrustworthiness. When such cues are present, suspicious cognitive and motivational reactions are triggered in victim‐sensitive individuals, and they behave preemptively selfish to avoid being exploited by others. Although functional at times, victim sensitivity does have dysfunctional side effects. The present article reviews recent findings regarding the SeMI model and sketches potential avenues for future research.
The effects of DELLAs on growth change with developmental stage and brassinosteroid levels
Authors: Stewart Lilley Jodi L. , Gan Yinbo , Graham Ian A. , Nemhauser Jennifer L. 
Published: July 2013

SummaryThere are two stages in photomorphogenesis. First, seedlings detect light and open their cotyledons. Second, seedlings optimize their light environment by controlled elongation of the seedling stem or hypocotyl. In this study, we used time‐lapse imaging to investigate the relationship between the brassinosteroid (BR) and gibberellin (GA) hormones across both stages of photomorphogenesis. During the transition between one stage and the other, growth promotion by BRs and GAs switched from an additive to a synergistic relationship. Molecular genetic analysis revealed unexpected roles for known participants in the GA pathway during this period. Members of the DELLA family could either repress or enhance BR growth responses, depending on developmental stage. At the transition point for seedling growth dynamics, the BR and GA pathways had opposite effects on DELLA protein levels. In contrast to GA‐induced DELLA degradation, BR treatments increased the levels of REPRESSOR of ga1‐3 (RGA) and mimicked the molecular effects of stabilizing DELLAs. In addition, DELLAs showed complex regulation of genes involved in BR biosynthesis, implicating them in BR homeostasis. Growth promotion by GA alone depended on the PHYTOCHROME INTERACTING FACTOR (PIF) family of master growth regulators. The effects of BR, including the synergistic effects with GA, were largely independent of PIFs. These results point to a multi‐level, dynamic relationship between the BR and GA pathways.
Anti‐D alloimmunization after D‐incompatible platelet transfusions: a 14‐year single‐institution retrospective review
Authors: O'Brien Kerry L. , Haspel Richard L. , Uhl Lynne 
Published: July 2013

BackgroundA small, but immunogenic dose of red blood cells (RBCs) may be contained in apheresis platelets (PLTs). Attempts are made to provide D− recipients with D− PLTs to prevent anti‐D alloimmunization and the potential for hemolytic disease of the fetus and newborn. Beth Israel Deaconess Medical Center has a policy that when necessary to transfuse D+ PLTs to D− patients, we recommend that RhIG be given when the patient is a woman of child‐bearing age or a potential liver transplant patient. We sought to retrospectively determine the rate of anti‐D formation after D‐incompatible apheresis PLT transfusions in those patients not receiving RhIG and not receiving D+ RBCs over a 14‐year period at our institution.Study Design and MethodsAll D− patients (626) who received D+ prestorage leukoreduced apheresis PLTs between January 1, 1997, and December 31, 2011, were identified. Those patients who received RhIG (45), D+ RBC transfusions (50), or stem cell transplantation from a D+ donor (16); had prior anti‐D (23); or had unresolved Rh at admission (8) were not eligible for analysis. Only those patients who had an antibody screen performed at least 4 weeks after the incipient PLT transfusion were evaluated (130).ResultsOf 130 eligible D− patients, 48% women and 57% immunocompetent, who received a total of 565 apheresis PLTs, none formed anti‐D.ConclusionThese findings support the use of D+ apheresis PLTs without RhIG irrespective of D status in all recipients.
Daytime Heat Transfer Processes over Mountainous Terrain
Authors: Schmidli Juerg 
Published: July 2013

Abstract
The daytime heat transfer mechanisms over mountainous terrain are investigated by means of large-eddy simulations over idealized valleys. Two- and three-dimensional topographies, corresponding to infinite and finite valleys, are used in order to evaluate the influence of the along-valley wind and the valley surroundings on the heat transfer processes. The atmosphere is coupled to an interactive land surface, allowing for dynamic feedback on the surface fluxes.
The valley heat budget is analyzed both from a local and bulk perspective, and the flow is Reynolds decomposed into its mean and turbulent component. The analysis clarifies recent issues of contention regarding the heating of the valley atmosphere. The flow decomposition allows one to clearly distinguish between the different heating processes: those associated with the mean flow, such as advection-induced cooling by the upslope flows and the warming induced by the compensating subsidence, and those associated with the turbulent motions. The latter include the warming of the mixed layer due to the convergence of the turbulent heat flux and cooling in the capping inversion due to overshooting thermals. The analysis from the bulk perspective confirms that the net effect of the thermally induced cross-valley circulation is to export heat out of the valley and away from the mountain ridge. The valley-volume effect is confirmed as the primary cause of enhanced diurnal temperature amplitudes in valleys. The results are robust with regard to the different topographies studied.
Vertical Heat Transport by Ocean Circulation and the Role of Mechanical and Haline Forcing
Authors: Zika Jan D. , Sijp Willem P. , England Matthew H. 
Published: July 2013

Abstract
Vertical transport of heat by ocean circulation is investigated using a coupled climate model and novel thermodynamic methods. Using a streamfunction in temperature–depth coordinates, cells are identified by whether they are thermally direct (flux heat upward) or indirect (flux heat downward). These cells are then projected into geographical and other thermodynamic coordinates. Three cells are identified in the model: a thermally direct cell coincident with Antarctic Bottom Water, a thermally indirect deep cell coincident with the upper limb of the meridional overturning circulation, and a thermally direct shallow cell coincident with the subtropical gyres at the surface. The mechanisms maintaining the thermally indirect deep cell are investigated. Sinking water within the deep cell is more saline than that which upwells, because of the coupling between the upper limb and the subtropical gyres in a broader thermohaline circulation. Despite the higher salinity of its sinking water, the deep cell transports buoyancy downward, requiring a source of mechanical energy. Experiments run to steady state with increasing Southern Hemisphere westerlies show an increasing thermally indirect circulation. These results suggest that heat can be pumped downward by the upper limb of the meridional overturning circulation through a combination of salinity gain in the subtropics and the mechanical forcing provided by Southern Hemisphere westerly winds.
Tropical Cyclone Data Impact Studies: Influence of Model Bias and Synthetic Observations
Authors: Reynolds Carolyn A. , Langland Rolf , Pauley Patricia M. , Velden Christopher 
Published: July 2013

AbstractThe impacts of assimilating dropwindsonde data and enhanced atmospheric motion vectors (AMVs) on tropical cyclone track forecasts are examined using the Navy global data assimilation and forecasting systems. Enhanced AMVs have the largest impact on eastern Pacific storms, while dropwindsonde data have the largest impact on Atlantic storms. Results in the western Pacific are mixed. Two western Pacific storms, Nuri and Jangmi, are examined in detail. For Nuri, dropwindsonde data and enhanced AMVs are at least as likely to degrade as to improve forecasts. For Jangmi, additional data improve track forecasts in most cases. An erroneous weakening of the forecasted subtropical high appears to contribute to the track errors for Nuri and Jangmi. Assimilation of enhanced AMVs systematically increases the analyzed heights in this region, counteracting this model bias. However, the impact of enhanced AMVs decreases rapidly as the model biases saturate at similar levels for experiments with and without the enhanced AMVs after the first few forecast days. Experiments are also conducted in which the errors assigned to synthetic tropical cyclone observations are increased. Moderate increases in the assigned errors improve track forecasts on average, but larger increases in the assigned errors produce mixed results. Both experiments allow for reductions in innovations and residuals when compared to dropwindsonde observations. These experiments suggest that a reformulation of the synthetic tropical cyclone observation scheme may lead to improved forecasts as more in situ and remote observations become available.
Near-Surface Vortex Structure in a Tornado and in a Sub-Tornado-Strength Convective-Storm Vortex Observed by a Mobile, W-Band Radar during VORTEX2
Authors: Tanamachi Robin L. , Bluestein Howard B. , Xue Ming , Lee Wen-Chau , Orzel Krzysztof A. , Frasier Stephen J. , Wakimoto Roger M. 
Published: July 2013

Abstract
As part of the Second Verification of the Origins of Rotation in Tornadoes Experiment (VORTEX2) field campaign, a very high-resolution, mobile, W-band Doppler radar collected near-surface (≤200 m AGL) observations in an EF-0 tornado near Tribune, Kansas, on 25 May 2010 and in sub-tornado-strength vortices near Prospect Valley, Colorado, on 26 May 2010. In the Tribune case, the tornado's condensation funnel dissipated and then reformed after a 3-min gap. In the Prospect Valley case, no condensation funnel was observed, but evidence from the highest-resolution radars in the VORTEX2 fleet indicates multiple, sub-tornado-strength vortices near the surface, some with weak-echo holes accompanying Doppler velocity couplets. Using high-resolution Doppler radar data, the authors document the full life cycle of sub-tornado-strength vortex beneath a convective storm that previously produced tornadoes. The kinematic evolution of these vortices, from genesis to decay, is investigated via ground-based velocity track display (GBVTD) analysis of the W-band velocity data. It is found that the azimuthal velocities in the Tribune tornado fluctuated in concert with the (dis)appearance of the condensation funnel. However, the dynamic pressure drop associated with the retrieved azimuthal winds was not sufficient to account for the condensation funnel. In the Prospect Valley case, the strongest and longest-lived sub-tornado-strength vortex exhibited similar azimuthal velocity structure to the Tribune tornado, but had weaker azimuthal winds. In both cases, the radius of maximum azimuthal wind was inversely related to the wind speed, and changes in the axisymmetric azimuthal component of velocity were consistent with independent indicators of vortex intensification and decay.
Spectroscopy and X-ray structure refinement of sekaninaite from Dolní Bory (Czech Republic)
Authors: Radica F. , Capitelli F. , Bellatreccia F. , Della Ventura G. , Cavallo A. , Piccinini M. , Hawthorne F. C. 
Published: July 2013

AbstractThe crystal chemistry of sekaninaite from Dolní Bory, Czech Republic, was characterized by a multimethod approach. Particular emphasis was put on the characterization of the channel constituents (i.e. H2O and CO2). Electron microprobe analysis shows the sample to be close to the Fe endmember [XFe = Fe/(Fe+Mg) = 94%) with significant Mn (1.48 wt.%) present; laser ablation mass-spectrometry showed the presence of 0.42 wt.% Li2O. H2O and CO2 contents (1.48 and 0.17 wt.%, respectively) were determined via secondary-ion mass-spectrometry. Sample homogeneity was checked by Fourier-transform infrared (FTIR) imaging using a microscope equipped with a focal plane array detector. Single-crystal FTIR spectroscopy confirmed the presence of two types of H2O groups in different orientations (with prevalence of the type II orientation), and that CO2 is oriented preferentially normal to the crystallographic c axis. Using the Beer-Lambert relation, integrated molar coefficients, εi, were calculated for both types of H2O (εi H2O[I] = 6000±2000; εi H2O[II] = 13000±1000) and for CO2 (εiCO2 = 2000±1000).
Kûngnât, revisited. A review of five decades of research into an alkaline complex in South Greenland, with new trace-element and Nd isotopic data
Authors: Upton B. G. J. , Macdonald R. , Odling N. , Rämö O. T. , Bagiński B. 
Published: July 2013

AbstractThe Kûngnât Complex (1275±1.8 Ma) in the Gardar Alkaline Province, South Greenland, cuts Archaean gneisses and comprises two intersecting syenitic stocks and a gabbroic ring-dyke. The magmas, with increasingly more primitive compositions, were emplaced successively by ring-faulting and roof stoping. The syenites are orthocumulates (cumulus alkali feldspar, olivine, pyroxene, titanomagnetite and apatite; intercumulus phases include alkali amphibole, biotite, quartz and calcite). In the well dissected earlier stock, a 2.2 km-thick layered sequence displays graded modal layering, feldspar lamination and cryptic layering. Modal layering in both stocks is directed mainly inwards at 35° – 50°. Heterogeneous nucleation of the cumulus assemblage, close to steep thermal boundary layers, is inferred. The modal layering is ascribed primarily to gravitational sorting aided by the large density differential between a) feldspar and b) Fe-rich silicates and oxides. Episodic collapse of cumulus + melt slurries contributed to inward-dipping crystal pediments on the chamber floors. The Ring-Dyke (up to 100 m wide) is nearly continuous through 360°. Kûngnât exhibits a compositional nearcontinuum from olivine gabbro through syenite intermediaries to alkali granite, ascribed to protracted assimilation/fractional crystallization processes. The most radiogenic Nd isotope data from Kûngnât (εNdivalues between –3.3 and –1.0) point to a lithospheric mantle source, whereas the most unradiogenic values imply enrichment inLREEby crustal contamination of the magmas.
Gel2DE - A software tool for correlation analysis of 2D gel electrophoresis data
Authors: Øye Ola Kristoffer , Jørgensen Katarina M , Hjelle Sigrun M , Sulen André , Ulvang Dag Magne , Gjertsen Bjørn Tore 
Published: July 2013

Abstract

Background
Two-dimensional gel electrophoresis (2DE) is a powerful technique for studying protein isoforms and their modifications. Existing commercial 2D image analysis tools rely on spot detection that limits analysis of complex protein profiles, e.g. spot appearance/disappearance or overlapping spots. Pixel-by-pixel correlation analysis, an analysis technique for identifying relations between protein patterns in gel images and external variables, can overcome such limitations in spot analysis.

Results
We have implemented the first publically available pixel-by-pixel correlation analysis tool, the software Gel2DE. 2D immunoblot time course analysis of p53 protein stabilization in response to ionizing irradiation shows that pixel-by-pixel analysis can yield an overall activation biosignature for p53, despite changing spots shape, size and position.

Conclusions
Pixel-by-pixel correlation of aligned 2D images permits analysis of complex protein patterns. We anticipate that the Gel2DE correlation software will be a useful tool for future bioinformatics discoveries through 2D gel electrophoresis.
Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae
Authors: Cui Hongli , Yu Xiaona , Wang Yan , Cui Yulin , Li Xueqin , Liu Zhaopu , Qin Song 
Published: July 2013

Abstract

Background
Xanthophylls, oxygenated derivatives of carotenes, play critical roles in photosynthetic apparatus of cyanobacteria, algae, and higher plants. Although the xanthophylls biosynthetic pathway of algae is largely unknown, it is of particular interest because they have a very complicated evolutionary history. Carotenoid hydroxylase (CHY) is an important protein that plays essential roles in xanthophylls biosynthesis. With the availability of 18 sequenced algal genomes, we performed a comprehensive comparative analysis of chy genes and explored their distribution, structure, evolution, origins, and expression.

Results
Overall 60 putative chy genes were identified and classified into two major subfamilies (bch and cyp97) according to their domain structures. Genes in the bch subfamily were found in 10 green algae and 1 red alga, but absent in other algae. In the phylogenetic tree, bch genes of green algae and higher plants share a common ancestor and are of non-cyanobacterial origin, whereas that of red algae is of cyanobacteria. The homologs of cyp97a/c genes were widespread only in green algae, while cyp97b paralogs were seen in most of algae. Phylogenetic analysis on cyp97 genes supported the hypothesis that cyp97b is an ancient gene originated before the formation of extant algal groups. The cyp97a gene is more closely related to cyp97c in evolution than to cyp97b. The two cyp97 genes were isolated from the green alga Haematococcus pluvialis, and transcriptional expression profiles of chy genes were observed under high light stress of different wavelength.

Conclusions
Green algae received a β-xanthophylls biosynthetic pathway from host organisms. Although red algae inherited the pathway from cyanobacteria during primary endosymbiosis, it remains unclear in Chromalveolates. The α-xanthophylls biosynthetic pathway is a common feature in green algae and higher plants. The origination of cyp97a/c is most likely due to gene duplication before divergence of green algae and higher plants. Protein domain structures and expression analyses in green alga H. pluvialis indicate that various chy genes are in different manners response to light. The knowledge of evolution of chy genes in photosynthetic eukaryotes provided information of gene cloning and functional investigation of chy genes in algae in the future.
The identification of 14 new genes for meat quality traits in chicken using a genome-wide association study
Authors: Sun Yanfa , Zhao Guiping , Liu Ranran , Zheng Maiqing , Hu Yaodong , Wu Dan , Zhang Lei , Li Peng , Wen Jie 
Published: July 2013

Abstract

Background
Meat quality is an important economic trait in chickens. To identify loci and genes associated with meat quality traits, we conducted a genome-wide association study (GWAS) of F2 populations derived from a local Chinese breed (Beijing-You chickens) and a commercial fast-growing broiler line (Cobb-Vantress).

Results
In the present study, 33 association signals were detected from the compressed mixed linear model (MLM) for 10 meat quality traits: dry matter in breast muscle (DMBr), dry matter in thigh muscle (DMTh), intramuscular fat content in breast muscle (IMFBr), meat color lightness (L*) and yellowness (b*) values, skin color L*, a* (redness) and b* values, abdominal fat weight (AbFW) and AbFW as a percentage of eviscerated weight (AbFP). Relative expressions of candidate genes identified near significant signals were compared using samples of chickens with High and Low phenotypic values. A total of 14 genes associated with IMFBr, meat color L*, AbFW, and AbFP, were differentially expressed between the High and Low phenotypic groups. These genes are, therefore, prospective candidate genes for meat quality traits: protein tyrosine kinase (TYRO3) and microsomal glutathione S-transferase 1 (MGST1) for IMFBr; collagen, type I, alpha 2 (COL1A2) for meat color L*; and RET proto-oncogene (RET), natriuretic peptide B (NPPB) and sterol regulatory element binding transcription factor 1 (SREBF1) for the abdominal fat (AbF) traits.

Conclusions
Based on the association signals and differential expression of nearby genes, 14 candidate loci and genes for IMFBr, meat L* and b* values, and AbF are identified. The results provide new insight into the molecular mechanisms underlying meat quality traits in chickens.