bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCSK9 Induces CD36 Degradation and Affects Long-Chain Fatty Acid Uptake and Triglyceride Metabolism in Adipocytes and in Mouse Liver
Authors: Demers Annie , Samami Samaneh , Lauzier Benjamin , Des Rosiers Christine , Sock Emilienne Tudor Ngo , Ong Huy , Mayer Gaetan 
Published: October 2015

Objective—
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes the degradation of the low-density lipoprotein receptor thereby elevating plasma low-density lipoprotein cholesterol levels and the risk of coronary heart disease. Thus, the use of PCSK9 inhibitors holds great promise to prevent heart disease. Previous work found that PCSK9 is involved in triglyceride metabolism, independently of its action on low-density lipoprotein receptor, and that other yet unidentified receptors could mediate this effect. Therefore, we assessed whether PCSK9 enhances the degradation of CD36, a major receptor involved in transport of long-chain fatty acids and triglyceride storage.

Approach and Results—

Overexpressed or recombinant PCSK9 induced CD36 degradation in cell lines and primary adipocytes and reduced the uptake of the palmitate analog Bodipy FL C
16
and oxidized low-density lipoprotein in 3T3-L1 adipocytes and hepatic HepG2 cells, respectively. Surface plasmon resonance, coimmunoprecipitation, confocal immunofluorescence microscopy, and protein degradation pathway inhibitors revealed that PCSK9 directly interacts with CD36 and targets the receptor to lysosomes through a mechanism involving the proteasome. Importantly, the level of CD36 protein was increased by >3-fold upon small interfering RNA knockdown of endogenous PCSK9 in hepatic cells and similarly increased in the liver and visceral adipose tissue of
Pcsk9
−/−
mice. In
Pcsk9
−/−
mice, increased hepatic CD36 was correlated with an amplified uptake of fatty acid and accumulation of triglycerides and lipid droplets.

Conclusions—
Our results demonstrate an important role of PCSK9 in modulating the function of CD36 and triglyceride metabolism. PCSK9-mediated CD36 degradation may serve to limit fatty acid uptake and triglyceride accumulation in tissues, such as the liver.
Time-Resolved C-Arm Computed Tomographic Angiography Derived From Computed Tomographic Perfusion Acquisition
Authors: Yang Pengfei , Niu Kai , Wu Yijing , Struffert Tobias , Dorfler Arnd , Schafer Sebastian , Royalty Kevin , Strother Charles , Chen Guang-Hong 
Published: October 2015

Background and Purpose—
Multimodal imaging using cone beam C-arm computed tomography (CT) may shorten the delay from ictus to revascularization for acute ischemic stroke patients with a large vessel occlusion. Largely because of limited temporal resolution, reconstruction of time-resolved CT angiography (CTA) from these systems has not yielded satisfactory results. We evaluated the image quality and diagnostic value of time-resolved C-arm CTA reconstructed using novel image processing algorithms.

Methods—
Studies were done under an Institutional Review Board approved protocol. Postprocessing of data from 21 C-arm CT dynamic perfusion acquisitions from 17 patients with acute ischemic stroke were done to derive time-resolved C-arm CTA images. Two observers independently evaluated image quality and diagnostic content for each case. ICC and receiver-operating characteristic analysis were performed to evaluate interobserver agreement and diagnostic value of this novel imaging modality.

Results—
Time-resolved C-arm CTA images were successfully generated from 20 data sets (95.2%, 20/21). Two observers agreed well that the image quality for large cerebral arteries was good but was more limited for small cerebral arteries (distal to M1, A1, and P1). receiver-operating characteristic curves demonstrated excellent diagnostic value for detecting large vessel occlusions (area under the curve=0.987–1).

Conclusions—
Time-resolved CTAs derived from C-arm CT perfusion acquisitions provide high quality images that allowed accurate diagnosis of large vessel occlusions. Although image quality of smaller arteries in this study was not optimal ongoing modifications of the postprocessing algorithm will likely remove this limitation. Adding time-resolved C-arm CTAs to the capabilities of the angiography suite further enhances its suitability as a one-stop shop for care for patients with acute ischemic stroke.
Modeling Rossby Wave Breaking in the Southern Spring Stratosphere
Authors: Guha Anirban , Mechoso Carlos R. , Konor Celal S. , Heikes Ross P. 
Published: October 2015

Abstract
Rossby wave breaking (RWB) plays a central role in the evolution of stratospheric flows. The generation and evolution of RWB is examined in the simple dynamical framework of a one-layer shallow-water system on a sphere. The initial condition represents a realistic, zonally symmetric velocity profile corresponding to the springtime southern stratosphere. Single zonal wavenumber Rossby waves, which are either stationary or traveling zonally with realistic speeds, are superimposed on the initial velocity profile. Particular attention is placed on the Lagrangian structures associated with RWB. The Lagrangian analysis is based on the calculation of trajectories and the application of a diagnostic tool known as the “M” function. Hyperbolic trajectories (HTs), produced by the transverse intersections of stable and unstable invariant manifolds, may yield chaotic saddles in M. Previous studies associated HTs with “cat’s eyes” generated by planetary wave breaking at the critical levels. HTs, and hence RWB, are found both outside and inside the stratospheric polar vortex (SPV). Significant findings are as follows: (i) stationary forcing produces HTs only outside of the SPV and (ii) eastward-traveling wave forcing can produce HTs both outside and inside of the SPV. In either case, HTs appear at or near the critical latitudes. RWB was found to occur inside the SPV even when the forcing was located completely outside. In all cases, the westerly jet remained impermeable throughout the simulations. The results suggest that the HT inside the SPV observed by de la Cámara et al. during the southern spring 2005 was due to RWB of an eastward-traveling wave of wavenumber 1.
Understanding Instabilities of Tropical Cyclones and Their Evolution with a Moist Convective Rotating Shallow-Water Model
Authors: Lahaye Noé , Zeitlin Vladimir 
Published: October 2015

Abstract
Instabilities of hurricane-like vortices are studied with the help of a rotating shallow-water model, including the effects of moist convection. Linear stability analysis demonstrates that dominant unstable modes are mixed Rossby–inertia–gravity waves. It is shown that, depending on fine details of the vorticity profile, a wavenumber selection of the instability may operate or not, leading in some cases to an unstable mode with a distinctively maximal growth rate and in other cases to an ensemble of unstable modes with close growth rates. Numerical simulations are performed in order to investigate nonlinear saturation of the instability and to understand the dynamical role of moisture. In agreement with previous studies, the authors confirm axisymmetrization of vorticity in the course of the development of the instability, which induces changes of intensity of the hurricane. In “dry” simulations, winds are intensified only inside the radius of maximum wind, while the maximum value of the wind decreases. “Moist precipitating” simulations (with and without evaporation) exhibit a net increase of winds, also at the radius of maximum wind, as compared to the dry simulations. Dynamical effects of moisture on the reorganization of the vortex and on the efficiency of inertia–gravity wave emission are quantified and shown to be considerable. Periodic bursts in the emission of waves related to the development of the unstable modes inside the vortex are evidenced, as well as the appearance of convectively coupled waves in the moist precipitating simulations with evaporation.
The Impact of Incongruous Lake Temperatures on Regional Climate Extremes Downscaled from the CMIP5 Archive Using the WRF Model
Authors: Spero Tanya L. , Nolte Christopher G. , Bowden Jared H. , Mallard Megan S. , Herwehe Jerold A. 
Published: October 2015

Abstract
The impact of incongruous lake temperatures is demonstrated using the Weather Research and Forecasting (WRF) Model to downscale global climate fields. Unrealistic lake temperatures prescribed by the default WRF configuration cause obvious biases near the lakes and also affect predicted extremes hundreds of kilometers from the lakes, especially during winter. Using these default temperatures for the Great Lakes in winter creates a thermally induced wave in the modeled monthly average sea level pressure field, which reaches southern Florida. Differences of more than 0.5 K in monthly average daily maximum 2-m temperature occur along that wave during winter. Noteworthy changes to temperature variability, precipitation, and mesoscale circulation also occur when the default method is used for downscaling. Consequently, improperly setting lake temperatures for downscaling could result in misinterpreting changes in regional climate and adversely affect applications reliant on downscaled data, even in areas remote from the lakes.
The 2011 Great Flood in Thailand: Climate Diagnostics and Implications from Climate Change
Authors: Promchote Parichart , Simon Wang S.-Y. , Johnson Paul G. 
Published: October 2015

Abstract
Severe flooding occurred in Thailand during the 2011 summer season, which resulted in more than 800 deaths and affected 13.6 million people. The unprecedented nature of this flood in the Chao Phraya River basin (CPRB) was examined and compared with historical flood years. Climate diagnostics were conducted to understand the meteorological conditions and climate forcing that led to the magnitude and duration of this flood. Neither the monsoon rainfall nor the tropical cyclone frequency anomalies alone was sufficient to cause the 2011 flooding event. Instead, a series of abnormal conditions collectively contributed to the intensity of the 2011 flood: anomalously high rainfall in the premonsoon season, especially during March; record-high soil moisture content throughout the year; elevated sea level height in the Gulf of Thailand, which constrained drainage; and other water management factors. In the context of climate change, the substantially increased premonsoon rainfall in CPRB after 1980 and the continual sea level rise in the river outlet have both played a role. The rainfall increase is associated with a strengthening of the premonsoon northeasterly winds that come from East Asia. Attribution analysis using phase 5 of the Coupled Model Intercomparison Project historical experiments pointed to anthropogenic greenhouse gases as the main external climate forcing leading to the rainfall increase. Together, these findings suggest increasing odds for potential flooding of similar intensity to that of the 2011 flood.
MOGREPS-UK Convection-Permitting Ensemble Products for Surface Water Flood Forecasting: Rationale and First Results
Authors: Golding Brian , Roberts Nigel , Leoncini Giovanni , Mylne Ken , Swinbank Richard 
Published: October 2015

ABSTRACT
Flooding is one of the costliest hazards in the United Kingdom. A large part of the annual flood damage is caused by surface water flooding that is a direct result of intense rainfall. Traditional catchment-based approaches to flood prediction are not applicable for surface water floods. However, given sufficiently accurate forecasts of rainfall intensity, with sufficient lead time, actions can be taken to reduce their impact. These actions require reliable information about severity and areas at risk that is clear and easily interpreted. The accuracy requirements, in particular, are very challenging, as they relate to prediction of intensities that occur only infrequently and that typically affect only small areas. In this paper, forecasts of intense rainfall from a new convection-permitting ensemble prediction system are evaluated using radar observations of intense rain and surface water flooding reports. An urban flooding case that occurred in Edinburgh in 2011 is first investigated and then a broader look is taken at performance through a 3-month period during the London Olympic and Paralympic Games in 2012. Conclusions are drawn about the value of the ensemble and the particular means of presenting the forecasts, and areas requiring further work are highlighted.
Prediction and Predictability of High-Impact Western Pacific Landfalling Tropical Cyclone Vicente (2012) through Convection-Permitting Ensemble Assimilation of Doppler Radar Velocity
Authors: Zhu Lei , Wan Qilin , Shen Xinyong , Meng Zhiyong , Zhang Fuqing , Weng Yonghui , Sippel Jason , Gao Yudong , Zhang Yunji , Yue Jian 
Published: October 2015

Abstract
The current study explores the use of an ensemble Kalman filter (EnKF) based on the Weather Research and Forecasting (WRF) Model to continuously assimilate high-resolution Doppler radar data during the peak-intensity stage of Tropical Cyclone (TC) Vicente (2012) before landfall. The WRF-EnKF analyses and forecasts along with the ensembles initialized from the EnKF analyses at different times were used to examine the subsequent evolution, three-dimensional (3D) structure, predictability, and dynamics of the storm. Vicente was an intense western North Pacific tropical cyclone that made landfall around 2000 UTC 23 July 2012 near the Pearl River Delta region of Guangdong Province, China, with a peak 10-m wind speed around 44 m s−1 along with considerable inland flooding after a rapid intensification process. With vortex- and dynamics-dependent background error covariance estimated by the short-term ensemble forecasts, it was found that the WRF-EnKF could efficiently assimilate the high temporal and spatial resolution 3D radar radial velocity to improve the depiction of the TC inner-core structure of Vicente, which in turn improved the forecasts of the track and intensity along with the associated heavy precipitation inland. The ensemble forecasts and sensitivity analyses were further used to explore the leading dynamics that controlled the prediction and predictability of track, intensity, and rainfall during and after its landfall. Results showed that TC Vicente’s intensity and precipitation forecasts were largely dependent on the initial relationship between TC intensity and location and the initial steering flow.
Ensemble Data Assimilation Using a Unified Representation of Model Error
Authors: Piccolo Chiara , Cullen Mike 
Published: October 2015

Abstract
A natural way to set up an ensemble forecasting system is to use a model with additional stochastic forcing representing the model error and to derive the initial uncertainty by using an ensemble of analyses generated with this model. Current operational practice has tended to separate the problems of generating initial uncertainty and forecast uncertainty. Thus, in ensemble forecasts, it is normal to use physically based stochastic forcing terms to represent model errors, while in generating analysis uncertainties, artificial inflation methods are used to ensure that the analysis spread is sufficient given the observations. In this paper a more unified approach is tested that uses the same stochastic forcing in the analyses and forecasts and estimates the model error forcing from data assimilation diagnostics. This is shown to be successful if there are sufficient observations. Ensembles used in data assimilation have to be reliable in a broader sense than the usual forecast verification methods; in particular, they need to have the correct covariance structure, which is demonstrated.
Complications of Pediatric Otitis Media
Authors: Lavin Jennifer M. , Rusher Thomas , Shah Rahul K. 
Published: October 2015

ObjectiveOtitis media (OM) is a common diagnosis in the pediatric population that is usually managed on an outpatient basis. A small proportion of children are admitted due to a complex disease course. The aim of this study was to investigate the demographics of those patients and the resources utilized during their admissions.Study DesignRetrospective review based on the 2009 Kids’ Inpatient Database.SettingNationwide administrative database.Subjects and MethodsA review based on the 2009 Kids’ Inpatient Database was conducted. Inclusion criteria were clinical modification codes for OM (ICD‐9 code 382). Data recorded included patient demographics, concurrent discharge diagnosis codes, length of stay, total charges, and frequency of procedures performed.ResultsThere were 61,783 (92,548 nationally weighted) admissions with OM, which were analyzed. The average age (SD) for the patients was 2.18 (3.49) years, and the average length of stay was 2.88 days. The majority (80.75%) of patients did not have to undergo a procedure during admission, whereas a small proportion (5.4%) underwent a major operating room procedure. There were 21 deaths recorded (0.03%). A diagnosis of mastoiditis, meningitis, venous sinus thrombosis, or intracranial abscess was associated with significantly increased length of stay, incidence of procedures, and total cost of admission.ConclusionsComplicated pediatric OM remains of concern requiring prompt and thorough management. Major complications include mastoiditis and meningitis, and unfortunately, fatalities still occur in patients with OM. An understanding of resource utilization and socioeconomic implications can identify and drive opportunities for targeted quality improvement.
Knee MRI patterns of bone marrow reconversion and relationship to anemia
Authors: Gonzalez Felix M , Mitchell Jason , Monfred Erica , Anguh Terence , Mulligan Michael 
Published: October 2015

Background Bone marrow changes are commonly encountered on knee magnetic resonance imaging (MRI). The clinical relevance of these changes, especially as they relate to anemia, has not been studied in a large patient series. Purpose To determine if the extent of bone marrow reconversion (BMR) can assist the radiologist in making recommendations for further evaluation for underlying anemia. Material and Methods This study included 457 patients who had knee MRI over a 2-year period. Bone marrow patterns in the distal femur, proximal tibia, and fibula were graded as follows: 1, homogeneous fatty marrow; 2, patchy red marrow in the distal femur; 3, patchy red marrow in the distal femur and tibia and/or fibula; and 4, complete BMR in the femoral, tibial, and/or fibular metaphyses. Results There was a statistically significant difference ( P < 0.001) between the hemoglobin concentration and BMR grades. In women, anemia (Hb ≤12.0 g/dL) was found in 31 of 127 (24%) patients with grade 1, 31 of 83 (37%) with grade 2, 24 of 64 (37%) with grade 3, and 12 of 24 (50%) with grade 4. In men, anemia (Hb ≤13.0 g/dL) was found in 33 of 125 (26%) patients with grade 1, 12 of 19 (63%) with grade 2, five of 14 (36%) with grade 3, but not in the one patient with grade 4. Conclusion Women demonstrating marrow changes of a grade 4 BMR pattern should get a laboratory assessment for anemia.
Barriers to the Collaboration Between Hematologists and Palliative Care Teams on Relapse or Refractory Leukemia and Malignant Lymphoma Patients’ Care
Authors: Morikawa Miharu , Shirai Yuki , Ochiai Ryota , Miyagawa Kiyoshi 
Published: October 2015

Background: Palliative care service (PCS) has been shown to be utilized less in patients with leukemia and malignant lymphoma than in those with solid tumors. Previous studies have suggested hematologists’ limited awareness of PCS as one of the reason for low PCS referral in hematology. However, little is known about such an awareness and potential barriers to collaboration between hematologists and PCS. Aim: The present study aimed to assess ematologists and palliative care specialists’ perception about the roles of the hospital-based palliative care team (HPCT) and the barriers to collaboration between hematologists and palliative care teams on relapse or refractory leukemia and malignant lymphoma patients’ care Materials and Methods: A qualitative study was conducted using semistructured interviews with hematologists and palliative care specialists recruited from a hospital that provides hematology and palliative care by the HPCT. Data were evaluated via content analysis. Results: The study included 11 hematologists and 10 palliative care specialists. Our results revealed that they shared many common perceptions about the roles and expectations of the HPCT. Additionally, 7 categories of barriers to collaboration were identified, including not feeling the need to refer, the difficulty in referral timing, the lack of aggressive approach, the negative image of the HPCT, the need for hematologic malignancy-oriented management, the lack of communication, and others. Conclusion: We have identified hematologists’ and palliative care specialists’ perceptions of the HPCT’s roles and the barriers to their collaboration. A better understanding of such barriers may lead to effective collaboration between hematologists and the HPCT.
The Skin Microbiome in Atopic Dermatitis and Its Relationship to Emollients
Authors: Lynde Charles W. , Andriessen Anneke , Bertucci Vince , McCuaig Catherine , Skotnicki Sandy , Weinstein Miriam , Wiseman Marni , Zip Catherine 
Published: October 2015

Background: Human-associated bacterial communities on the skin, skin microbiome, likely play a central role in development of immunity and protection from pathogens. In atopic patients, the skin bacterial diversity is smaller than in healthy subjects. Objective: To review treatment strategies for atopic dermatitis in Canada, taking the skin microbiome concept into account. Methods: An expert panel of 8 Canadian dermatologists explored the role of skin microbiome in clinical dermatology, specifically looking at atopic dermatitis. Results: The panel reached consensus on the following: (1) In atopic patients, the skin microbiome of lesional atopic skin is different from nonlesional skin in adjacent areas. (2) Worsening atopic dermatitis and smaller bacterial diversity are strongly associated. (3) Application of emollients containing antioxidant and antibacterial components may increase microbiome diversity in atopic skin. Conclusion: The skin microbiome may be the next frontier in preventive health and may impact the approach to atopic dermatitis treatment.
Endoscopic Treatment for Persistent Hematospermia: A Novel Technique Using a Holmium Laser
Authors: Oh T. H. , Seo I. Y. 
Published: October 2015

Purpose: We present our endoscopic technique for treating ejaculatory duct and seminal vesicle diseases with a holmium laser. Materials and methods: Fifteen patients with persistent hematospermia were enrolled in this study from June 2007 to April 2014. All patients had failed medical treatments. All patients were evaluated with transrectal ultrasound and pelvic computed tomography or magnetic resonance imaging. We performed endoscopic treatment with a semi-rigid ureteroscope after dilation using a guidewire and ureteral serial dilator. A holmium laser was used to incise the obstructed ejaculatory duct, coagulate hemorrhagic mucosa, and fragment stones in the ejaculatory duct or seminal vesicles. Stones were removed using a basket and forceps. Results: The mean duration of hematospermia was 30.6 months. Mean patient age was 45.3 years. The mean serum levels of prostate-specific antigen and testosterone were 1.36 and 4.95 ng/mL, respectively. No operative complications were encountered. Mean operative time was 35.4 min. Seven patients had ejaculatory duct or seminal vesicle stones, which were subsequently determined to be carbonate apatite, mucin, struvite, and calcium oxalate dehydrate stones. Mean duration of follow-up was 32.1 months. Although two patients showed recurrent hematospermia 11 and 12 months after the operation, hematospermia resolved in 13 patients (86.7%). The infertile patient showed an improved semen finding and had a successful pregnancy. Conclusion: Endoscopic treatment using a holmium laser is minimally invasive and was effective for treating ejaculatory duct and seminal vesicle diseases, which are the main cause of hematospermia.