bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the pangas (Pangasianodon hypophthalmus) cultivation sustainability in cages at the Ratargul Freshwater Swamp Forest of Bangladesh
Authors: Kunda Mrityunjoy , Kawsar Md. Abu , Adikari Diponkor , Rishan Sakib Tahmid , Harun‐Al‐Rashid Ahmed , Pandit Debasish 
Published: July 2024

AbstractDetermining the most appropriate stocking density for a specific fish species in an ambient condition is essential for successful cage aquaculture in terms of profitability which is mainly governed by growth. The current study was conducted to ascertain which stocking density gives the best result of widely cultured species in cage aquaculture, pangas (Pangasianodon hypophthalmus), at swamp forest environment of Ratargul Freshwater Swamp Forest, Bangladesh in terms of growth, production and economic return. Fish were reared for 93 days at three stocking densities, namely 30, 40 and 50 fish/m3 which were denoted as FSD30, FSD40 and FSD50, respectively, containing three replications of each. Growth and yield comparisons indicated that the stocking density of fish had significant (p < 0.05) effects on growth rate and productivity. Fish cultivated at the maximum density (FSD50) had lower mean weights than fish reared at lower densities. The significantly highest mean weights (376.58 ± 14.65 g) and benefit–cost ratio (BCR) (1.617 ± 0.035) obtained from FSD30 show the suitability of this density in terms of fish growth and profitability of pangas in swamp forest environment.
Reconfigurable liquids enabled by dynamic covalent chemistry
Authors: Li Kaijuan , Luo Yuzheng , Wen Yunhui , Shan Wenrui , Shi Shaowei 
Published: July 2024

AbstractNanoparticle surfactants (NPSs) that form via the reversible non‐covalent interactions between nanoparticles (NPs) and polymer ligands at the oil‐water interface have received great attention in constructing structured liquids with unique stimuli‐responsiveness. Introducing dynamic covalent interactions to generate NPSs is expected to achieve a balance between high mechanical strength and dynamic responsiveness of the interfacial assemblies. Here, we present the formation, assembly, and jamming of a new type of NPS by the co‐assembly between polydopamine NPs (PDA NPs) and poly(styrene‐co‐methacrylamidophenylboronic acid) at the oil‐water interface. Dynamic covalent boronate ester bonds form in situ at the interface and show multiple responsiveness when applying stimuli such as pH, H2O2, and temperature, allowing the controlled assembly/jamming of NPSs and reconfiguration of liquid constructs. Due to the photothermal property of PDA NPs, the temperature responsiveness of boronate ester bonds can also be triggered by irradiating the biphasic system with near‐infrared (NIR) light. Moreover, when bringing two droplets encapsulated with NPSs into contact and irradiating the contact area by NIR, thermal welding of droplets can be realized, offering a straightforward to construct droplet networks and modular liquid devices.
Proposed complementary osteological indicators: Advancing the estimation of puberty stages in Bioarcheology
Authors: Bareggi Alessia , Giuffra Valentina , Riccomi Giulia 
Published: July 2024

AbstractObjectivesThe study of puberty is a well‐established area of bioarcheological research, which greatly enhances our understanding of adolescence and growth in the past. Since the publications of Shapland and Lewis' works, which have become “standards” for estimating puberty in skeletal material, no additional osteological indicators of puberty have been proposed. Nevertheless, clinical practice constantly develops skeletal maturation markers that could be useful in bioarcheology. This study aims to assess the applicability and reliability of novel puberty indicators as a complementary tool to estimate puberty in skeletal remains.Materials and MethodsFour new maturation markers including spheno‐occipital synchondrosis, humeral head ossification, calcaneal apophysis ossification, and mandibular premolar mineralization were selected and applied to a sample of 85 adolescents from pre‐Roman southern Italy (Pontecagnano, 7th–4th BCE).ResultsDespite some limits in adapting the original clinical methods to osteoarcheological material, the use of these novel skeletal indicators had moderate to excellent scoring repeatability and an overall high agreement with the puberty and menarche status previously estimated with standard methods. These results encourage us to apply these markers in bioarcheology. In some cases, minor adaptations of the original scoring systems are suggested to enhance reliability.DiscussionIncluding the proposed indicators in routine puberty data collection allows us to refine puberty estimation and improve the ability to identify key growth milestones in poorly preserved skeletons. Further application to osteological collections with diverse chronology and geographical differences is needed to assess how and to what extent the newly proposed maturation markers perform.
The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain
Authors: Kiani Faisal Ayub , Li Hao , Guo Panpan , Zhang Qiulin , Abouelfetouh Mahmoud M. , Ding Mingxing , Ding Yi 
Published: July 2024

AbstractBackgroundExisting remedial approaches for relieving neuropathic pain (NPP) are challenging and open the way for alternative therapeutic measures such as electroacupuncture (EA). The mechanism underlying the antinociceptive effects of repeated EA sessions, particularly concerning the regulation of the Adora3 receptor and its associated enzymes, has remained elusive.MethodsThis study used a mouse model of spared nerve injury (SNI) to explore the cumulative analgesic effects of repeated EA at ST36 (Zusanli) and its impact on Adora3 regulation in the spinal cord dorsal horn (SCDH). Forty‐eight male mice underwent SNI surgery for induction of neuropathic pain and were randomly assigned to the SNI, SNI + 2EA, SNI + 4EA, and SNI + 7EA groups. Spinal cord (L4–L6) was sampled for immunofluorescence, adenosine (ADO) detection and for molecular investigations following repeated EA treatment.ResultsFollowing spared nerve injury (SNI), there was a significant decrease in mechanical withdrawal thresholds (PWTs) and thermal nociceptive withdrawal latency (TWL) in the ipsilateral hind paw on the third day post‐surgery, while the contralateral hind paw PWTs showed no significant changes. On subsequent EA treatments, the SNI + EA groups led to a significant increase in pain thresholds (p < 0.05). Repeated EA sessions in SNI mice upregulated Adenosine A3 (Adora3) and cluster of differentiation‐73 (CD73) expression while downregulating adenosine deaminase (ADA) and enhancing neuronal instigation in the SCDH. Colocalization analysis of Neun‐treated cells revealed increased Adora3 expression, particularly in the SNI + 7EA group.ConclusionsIn conclusion, cumulative electroacupuncture treatment reduced neuropathic pain by regulating Adora3 and CD73 expression, inhibiting ADA and most likely increasing neuronal activation in the SCDH. This study offers a promising therapeutic option for managing neuropathic pain, paving the way for further research.
Third Metal Ion Dictates the Catalytic Activity of the Two‐Metal‐Ion Pre‐Ribosomal RNA‐Processing Machinery
Authors: Borišek Jure , Aupič Jana , Magistrato Alessandra 
Published: July 2024

AbstractNucleic acid processing enzymes use a two‐Mg2+‐ion motif to promote the formation and cleavage of phosphodiester bonds. Yet, recent evidence demonstrates the presence of spatially conserved second‐shell cations surrounding the catalytic architecture of proteinaceous and RNA‐dependent enzymes. The RNase mitochondrial RNA processing (MRP) complex, which cleaves the ribosomal RNA (rRNA) precursor at the A3 cleavage site to yield mature 5′‐end of 5.8S rRNA, hosts in the catalytic core one atypically‐located Mg2+ ion, in addition to the ions forming the canonical catalytic motif. Here, we employ biased quantum classical molecular dynamics simulations of RNase MRP to discover that the third Mg2+ ion inhibits the catalytic process. Instead, its displacement in favour of a second‐shell monovalent K+ ion propels phosphodiester bond cleavage by enabling the formation of a specific hydrogen bonding network that mediates the essential proton transfer step. This study points to a direct involvement of a transient K+ ion in the catalytic cleavage of the phosphodiester bond and implicates cation trafficking as a general mechanism in nucleic acid processing enzymes and ribozymes.
In‐Situ Solid Electrolyte Interface via Dual Reaction Strategy for Highly Reversible Zinc Anode
Authors: Xu Peiwen , Xu Mi , Zhang Jie , Zou Jiabin , Shi Yue , Luo Dan , Wang Dongdong , Dou Haozhen , Chen Zhongwei 
Published: July 2024

AbstractIn situ construction of solid electrolyte interfaces (SEI) is an effective strategy to enhance the reversibility of zinc (Zn) anodes. However, in situ SEI to afford high reversibility under high current density conditions (≥20 mA cm−2) is highly desired yet extremely challenging. Herein, we propose a dual reaction strategy of spontaneous electrostatic reaction and electrochemical decomposition for the in situ construction of SEI, which is composed of organic‐rich upper layer and inorganic‐rich inner layer. Particularly, in situ SEI performs as “growth binder“ at small current density and “orientation regulator” at high current density, which significantly suppresses side reactions and dendrite growth. The in situ SEI affords the record‐breaking reversibility of Zn anode under practical conditions, Zn//Zn symmetric cells can stably cycle for over 1300 h and 400 h at current densities of 50 mA cm−2 and 100 mA cm−2, respectively, showcasing an exceptional cumulative capacity of 67.5 Ah cm−2. Furthermore, the practicality of this in situ SEI is verified in Zn//PANI pouch cells with high mass loading of 25.48 mg cm−2. This work provides a universal strategy to design advanced SEI for practical Zn‐ion batteries.
From Cyclopropene to Housane Derivatives Via Intramolecular Cyclopropanation
Authors: Coto Darío , Suárez‐García David , Mata Sergio , Fernández Israel , López Luis A. , Vicente Rubén 
Published: July 2024

AbstractThe synthesis of housane derivatives from cyclopropenes is described. Under rhodium(II) catalysis, cyclopropenylvinyl carbinols can regioselectively generate a carbene intermediate which undergoes an intramolecular cyclopropanation to form a housane, a skeleton with similar ring strain as the cyclopropene precursor. The procedure shows a remarkable broad scope and efficiency. Moreover, the method served to prepare man‐made housane‐containing terpene derivatives, which are not accessible by Nature.
Modulating Hydrogen Adsorption by Unconventional p–d Orbital Hybridization over Porous High‐Entropy Alloy Metallene for Efficient Electrosynthesis of Nylon‐6 Precursor
Authors: Sheng Youwei , Xie Jiangwei , Yang Ruidong , Yu Hongjie , Deng Kai , Wang Jianguo , Wang Hongjing , Wang Liang , Xu You 
Published: July 2024

AbstractRenewable electricity driven electrosynthesis of cyclohexanone oxime (C6H11NO) from cyclohexanone (C6H10O) and nitrogen oxide (NOx) is a promising alternative to traditional environment‐unfriendly industrial technologies for green synthesis of C6H11NO. Precisely controlling the reaction pathway of the C6H10O/NOx‐involved electrochemical reductive coupling reaction is crucial for selectively producing C6H11NO, which is yet still challenging. Herein, we report a porous high‐entropy alloy PdCuAgBiIn metallene (HEA‐PdCuAgBiInene) to boost the electrosynthesis of C6H11NO from C6H10O and nitrite, achieving a high Faradaic efficiency (47.6 %) and almost 100 % yield under ambient conditions. In situ Fourier transform infrared spectroscopy and theoretical calculations demonstrate that unconventional orbital hybridization between d‐block metals and p‐block metals could regulate the local electronic structure of active sites and induce electron localization of electron‐rich Pd sites, which tunes the active hydrogen supply, facilitates the generation and enrichment of key intermediates NH2OH* and C6H10O*, and efficiently promotes their C−N coupling to selectively produce C6H11NO.
Total Synthesis of (±)‐Baphicacanthcusine A Enabled by Sequential Ring Contractions
Authors: Sinclair Paul P. , Sarpong Richmond 
Published: July 2024

AbstractReported herein is the first total synthesis of the poly‐pseudoindoxyl natural product baphicacanthcusine A. The synthesis leverages the oxidative rearrangement of indoles to pseudoindoxyls to install vicinal pseudoindoxyl heterocycles in a diastereoselective manner. Key steps include an acid‐mediated cyclization/indole transposition, two diastereoselective oxidative ring contractions, and a site‐selective C−H oxygenation. The synthesis of the oxidation precursors was guided by recognition of an element of hidden symmetry. This work provides a foundation for the chemical synthesis of other poly‐pseudoindoxyl alkaloids.
Engineering Epsilon‐Near‐Zero Media with Waveguides
Authors: Li Peihang , Yan Wendi , Wang Shuyu , Fu Pengyu , Zhang Yongjian , Li Yue 
Published: July 2024

AbstractEpsilon‐Near‐Zero (ENZ) media have attracted widespread interest due to their unique electromagnetic properties, which have brought distinctive characteristics and phenomena, such as spatiotemporal decoupling, supercoupling and tunneling, constant phase transmission, near‐field enhancement, and so on. However, these ENZ characteristics are existed in natural plasmonic materials at their intrinsic plasma frequencies and accompanied by significant losses, thus limiting their applications in engineering. Different from the effect ENZ media with artificially periodic structures, the waveguide ENZ media offers a promising platform with non‐periodic architectures. Unlike the natural plasmonic materials and the periodic‐structured ENZ media, the waveguide ENZ media utilizes waveguide dispersion to achieve effective ENZ characteristics and phenomena with lower loss and smaller dimensions. This review begins with an exploration of the fundamental properties of the waveguide ENZ media and then introduces the design principles of different ENZ‐based electromagnetic devices. Finally, the review concludes with the challenges and potential development directions encountered by the ENZ media in the realm of electromagnetic applications.
Depletion of lipid storage droplet‐1 delays endoreplication progression and induces cell death in Drosophila salivary gland
Authors: Nguyen Yen D. H. , Pham Tuan L. A. , Nishihara Taisei , Kamei Kaeko , Tran Duy Binh 
Published: July 2024

AbstractPerilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet‐1 (LSD‐1) is a lipid storage droplet membrane surface‐binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD‐1 during specific tissue development remains under investigation. This study demonstrated the role of LSD‐1 in salivary gland development. Knockdown of Lsd‐1 in the salivary gland was established using the GAL4/UAS system. The third‐instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD‐1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd‐1 genetically interacted with Myc in the third‐instar larvae. These results demonstrate that LSD‐1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD‐1 in regulating salivary gland development and the interaction between LSD‐1 and Myc.
Quinazoline‐oxindole hybrids as angiokinase inhibitors and anticancer agents: Design, synthesis, biological evaluation, and molecular docking studies
Authors: Syam Yasmin M. , Abd El‐Karim Somaia S. , Abdel‐Mohsen Heba T. 
Published: July 2024

AbstractTwo new sets of quinazoline‐oxindole 8a–l and quinazoline‐dioxoisoindoline 10a–d hybrids were designed as type II angiokinase inhibitors and anticancer agents. The design strategy was adjusted to account for the quinazoline scaffold's placement in the target kinases' hinge region, where it would form hydrogen bonding and hydrophobic interactions with the important amino acids to stabilize it, and the amide group's occupation in the gate region, which would direct the oxindole scaffold toward the hydrophobic back pocket. The two sets of quinazolines 8a–l and 10a–d displayed pronounced inhibitory activity on VEGFR‐2 (IC50 = 0.46–2.20 µM). The quinazoline‐oxindole hybrids 8d, 8f, and 8h displayed IC50 = 0.46, 0.49, and 0.49 µM, respectively. Compound 8f demonstrated potent multikinase activity with IC50 values of 0.95 and 0.67 µM against FGFR‐1 and BRAF, respectively. Additionally, compound 8f showed significant anticancer activity against National Cancer Institute's cancer cell lines, with GI50 reaching 1.21 µM. Analysis of the impact of compound 8f on the MDA‐MB‐231 cell line's cell cycle and apoptosis revealed that 8f stalled the cell cycle at the G2/M phase and promoted its necrosis.
Nickel Complexes Bearing Quinoline Derived NNS Donor Ligands as Catalytic Activators for N‐Alkylation of Anilines with Alcohols
Authors: Tanwar Deepika , Mahala Suman , Ahluwalia Deepali , Bhuvanesh Nattamai , Joshi Hemant , Kumar Umesh 
Published: July 2024

AbstractHerein, we have reported a new series of NNS‐donor ligands coordinated Ni(II) complexes and utilized them as catalytic activator to synthesize N‐alkylated amines and 1,2‐disubstituted benzimidazoles. The reaction of thiophenol/4‐chlorothiophenol/4‐methylthiophenol/4‐methoxythiophenol with 2‐bromo‐N‐quinolin‐8‐yl‐acetamide in presence of sodium hydroxide in ethanol at 80 °C gave [C9H6N‐NH−C(O)−CH2‐S−Ar] [Ar=C6H5 (L1); C6H4Cl‐4 (L2); C6H4Me‐4 (L3) and C6H4‐OMe‐4 (L4)], respectively. The corresponding reaction of L1‐L4 with Ni(OAc)2 in methanol at 80 °C for 3 hours resulted in octahedral nickel complexes [(L1‐H)2Ni] (C1), [(L2‐H)2Ni] (C2), [(L3‐H)2Ni] (C3), and [(L4‐H)2Ni] (C4), respectively. All compounds have been characterized by micro and spectroscopic analysis. The molecular structure of complexes C1–C3 has also been determined by single crystal X‐ray diffraction data. The utility of complexes C1–C4 were evaluated for the N‐alkylation of aniline with benzyl alcohols, and for 1,2‐disubstituted benzimidazoles synthesis. The obtained results indicate that complex C1 showed better catalytic activity in both N‐alkylation of amines with benzyl alcohols [catalyst loading: 2.0 mol %; Yield up to 92 %], and for 1,2‐disubstituted benzimidazoles derivatives [catalyst loading: 2.0 mol %; Yield up to 94 %)]. The mechanistic studies suggested that the reaction works through hydrogen borrowing from benzyl alcohol and its subsequent utilization for in situ reduction of imine. The experimentally observed catalytic reactivity patterns of complexes C1–C4 have found in good agreement with the HOMO‐LUMO energy gaps obtained by DFT analysis of corresponding complexes.
H−/L∞UIO‐based fault detection and isolation design in finite‐frequency domain for nonlinear systems
Authors: Gao Sheng , Zhang Wei , Jin Bopi , Ma Siyuan 
Published: July 2024

AbstractThe issue of fault detection and isolation design in the finite‐frequency domain for discrete‐time Lipschitz nonlinear systems subjected to actuator faults and disturbances is investigated. A bank ofH−/L∞unknown input observers (UIOs) is established using the generalized observer scheme to generate residuals that are insensitive to a specific actuator fault but sensitive to the other actuator faults. Furthermore, in the finite‐frequency domain, theH−andL∞performance indices are simultaneously used to improve the fault detection sensitivity and attenuate the influence of disturbances on the residuals, respectively. The design conditions for the bank ofH−/L∞UIOs are derived from the generalized Kalman–Yakubovich–Popov lemma and converted into an optimization problem constrained by linear matrix inequalities to solve the design matrices more easily. To diagnose actuator faults, a fault detection and isolation scheme is established using the time‐varying threshold from theL∞performance analysis. Finally, the effectiveness of the fault detection and isolation scheme using the bank ofH−/L∞UIOs is validated by the simulation of two examples.
Architecture of RabL2‐associated complexes at the ciliary base: A structural modeling perspective
Authors: Boegholm Niels , Petriman Narcis A. , Tanvir Niaj M. , Lorentzen Esben 
Published: July 2024

AbstractCilia are slender, micrometer‐long organelles present on the surface of eukaryotic cells. They function in signaling and locomotion and are constructed by intraflagellar transport (IFT). The assembly of IFT complexes into so‐called IFT trains to initiate ciliary entry at the base of the cilium remains a matter of debate. Here, we use structural modeling to provide an architectural framework for how RabL2 is anchored at the ciliary base via CEP19 before being handed over to IFT trains for ciliary entry. Our models suggest that the N‐terminal domain of CEP43 forms a homo‐dimer to anchor at the subdistal appendages of cilia through a direct interaction with CEP350. A long linker region separates the N‐terminal domain of CEP43 from the C‐terminal domain, which captures CEP19 above the subdistal appendages and close to the distal appendages. Furthermore, we present a structural model for how RabL2‐CEP19 associates with the IFT‐B complex, providing insight into how RabL2 is handed over from CEP19 to the IFT complex. Interestingly, RabL2 association with the IFT‐B complex appears to induce a significant conformational change in the IFT complex via a kink in the coiled‐coils of the IFT81/74 proteins, which may prime the IFT machinery for entry into cilia.
The physical basis of analog‐to‐digital signal processing in the EGFR system—Delving into the role of the endoplasmic reticulum
Authors: Kreplin Laura Zoe , Arumugam Senthil 
Published: July 2024

AbstractReceptor tyrosine kinases exhibit ligand‐induced activity and uptake into cells via endocytosis. In the case of epidermal growth factor (EGF) receptor (EGFR), the resulting endosomes are trafficked to the perinuclear region, where dephosphorylation of receptors occurs, which are subsequently directed to degradation. Traveling endosomes bearing phosphorylated EGFRs are subjected to the activity of cytoplasmic phosphatases as well as interactions with the endoplasmic reticulum (ER). The peri‐nuclear region harbors ER‐embedded phosphatases, a component of the EGFR‐bearing endosome‐ER contact site. The ER is also emerging as a central player in spatiotemporal control of endosomal motility, positioning, tubulation, and fission. Past studies strongly suggest that the physical interaction between the ER and endosomes forms a reaction “unit” for EGFR dephosphorylation. Independently, endosomes have been implicated to enable quantization of EGFR signals by modulation of the phosphorylation levels. Here, we review the distinct mechanisms by which endosomes form the logistical means for signal quantization and speculate on the role of the ER.