bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hubungan Pemberian Reward, Keselamatan dan Kesehatan Kerja Terhadap Peningkatan Kinerja Pada Pekerja PT. Socfindo Perkebunan Tanah Gambus Kabupaten Batu Bara
Authors: Wardani Minati Eka 
Published: July 2024

Pemberian Reward, Keselamatan dan Kesehatan Kerja berhubungan erat dengan tingkat kinerja karyawan . Pemberian reward dapat memicu motivasi bekerja serta dengan penerapan K3 yang sehat dan aman meningkatkan kualitas dan tingkat kinerja. Tujuan penelitian adalah untuk mengetahui hubungan pemberian reward, serta K3 terhadap peningkatan kinerja pada PT. Socfindo Perkebunan Tanah Gambus Kabupaten Batu Bara. Jenis penelitian kuantitatif dengan pendekatan cross sectional. Populasi pada penelitian ini yaitu seluruh pekerja pabrik yang ada di PT.Socfindo berjumlah 100 pekerja. Teknik pengambilan sampel menggunakan purposive sampling sebanyak 50 pekerja. Data yang diperoleh di uji menggunakan univariat dengan distribusi frekuensi dan sentral sedangkan analisis bivariat memakai pengujian simultan menggunakan uji annova. Hasil penelitian diperoleh bahwa terdapat hubungan yang siginifikan dengan nilai F hitung (9,184) F tabel (3,19) dan niali p (0,000) α (0,05) dengan demikian Ha diterima. Kesimpulan penelitian ini adalah keberhasilan suatu perusahaan dalam mencapai tujuannya akan tergantung pada seberapa baik sumber daya manusianya dikembangkan. Disarankan perusahaan untuk lebih fokus pada kesehatan dan keselamatan pekerja baik internal (pengetahuan) maupun eksternal (pengawasan) tempat kerja, serta bagaimana mengembangkan budaya keselamatan kerja. Perusahaan juga perlu memperhatikan reward yang diberikan kepada karyawan, baik berwujud maupun tidak berwujud, dengan harapan mampu meningkatkan motivasi kerja karyawan dan membantu perusahaan untuk meraih tujuan akhirnya
Tinjauan Faktor Penyebab Dikembalikannya Berkas Klaim BPJS Pasien Rawat Inap di RSUD Raden Mattaher Jambi
Authors: Putri Dwi Tania , Viatiningsih Wiwik , Dewi Deasy Rosmala , Yulia Noor 
Published: July 2024

Pendahuluan: Jaminan kesehatan nasional (JKN) dalam pemeliharaan kesehatan masyarakat menunjuk BPJS kesehatan sebagai penyelenggara pembayaran program jaminan kesehatan. Dalam pelaksanaannya BPJS menggunakan metode pembayaran sistem pembiayaan prospektif INA-CBGs, rumah sakit mengajukan klaim atas layanan kesehatan yang telah diberikan kepada peserta JKN nantinya biaya pelayanan tersebut akan dibayar oleh BPJS Kesehatan. Proses pengajuan klaim BPJS Kesehatan melalui tahapan verifikasi yaitu verifikasi administrasi kepesertaan, verifikasi administrasi pelayanan dan verifikasi pelayanan kesehatan. Tujuan: Tujuan dari penelitian ini adalah agar mengetahui faktor penyebab dikembalikannya berkas klaim BPJS pasien rawat ianp di RSUD Raden Mattaher Jambi. Metode: Metode penelitian yang digunakan adalah kuantitatif deskriptif. Sampel adalah 110 berkas klaim. Pengumpulan data menggunakan observasi dan wawancara dengan instrumen daftar tilik dan pedoman wawancara. Hasil: Hasil penelitian menemukan RS belum memiliki SPO yang mengatur pelaksanaan klaim BPJS, faktor yang menyebabkan pengembalian klaim yaitu ketidaksesuaian SEP dengan data yang di upload 3,6% (4 klaim), ketidaklengkapan softfile yang di upload dengan persyaratan 3,6% (4 klaim), kesalahan penulisan tanggal masuk 2,7% (3 klaim), kesalahan nama tindakan di lembar laporan tindakan 1,9% (2 klaim), ketidaksesuaian kode diagnosa utama prosedur 30% (33 klaim), ketidaksesuaian kode diagnosa sekunder 18,2% (20 klaim), ketidaksesuaian kelas perawatan 12,7% (14 klaim), tidak ada hasil penunjang 20,9% (23 klaim), tidak tertulis riwayat penyakit 6,4% (7 klaim). Kesimpulan: RSUD Raden Mattaher Jambi belum memiliki SPO yang mengatur proses pelaksanaan klaim BPJS. Namun dalam pelaksanaan pengklaiman sudah menggunakan Petunjuk Teknis Verifikasi Klaim yang dikeluarkan oleh BPJS.
Analisis Riset Hipervirulensi dan Multi Drug Resisten Klebsiella Pneumoniae: Studi Bibliometrik Berbasis VOSviewer
Authors: Sodiqah Yani , Aprilani Hattah Dhian Karina , Muchtar Amrizal 
Published: July 2024

Latar Belakang: Sifat co-transfer Gen pengkode hipervirulensi (hvKP) dan resistensi antibiotik (MDR-KP) Klebsiella Pneumoniae memfasilitasi konvergensi, membentuk superbug yang berpotensi menyebabkan wabah dengan gejala berat dan tak terobati. Untuk mengidentifikasi tema riset terbaru mengenai hvKP-MDR-KP, digunakan analisis berbasis co-occurance dari VOSviewer. Studi ini membantu mengubah metadata publikasi menjadi visualisasi kata kunci sesuai tema penelitian atau cluster pada disiplin ilmu tertentu, dan dianalisis berbasis publikasi dan keilmuan.
Isi: Riset hvKP dan MDR-KP mulai meningkat sejak tahun 2020, didominasi dari negara China (38 %), diteliti di berbagai bidang, terutama pada bidang mikrobiologi (41%), infeksi (38%) dan farmakologi (28%). Dari 39 artikel terpilih 39 kata kunci co-occurance, membentuk 5 cluster yang saling terkait.
Kesimpulan: Resistensi Carbapenem, prevalensi dan hipervirulensi Klebsiella pneumoniae merupakan kata kunci yg paling aktual, sehingga sangat disarankan menjadi tema riset terkait hvKP dan MDR-KP selanjutnya.
Evidence for the Presence of β2m hom Target Proteins and HLA in Humanized Transgenic Mice of HLA-A*02:01, HLA-B*07:02, and HLA-C*07:02 Lines
Authors: Karkischenko V. N. , Berzina A. G. , Petrova N. V. , Pomytkin I. A. , Glotova E. S. , Petrov D. V. , Taboyakova L. A. , Bolotskih L. A. , Laryushina N. А. 
Published: July 2024

Human leukocyte antigen plays a primary role in the formation of immune response and pathogenesis of diseases of various etiologies, including the development of negative side effects induced by pharmacological agents. Modern pharmacosafety standards require improvement of existing test systems to conduct high-quality preclinical studies. A number of humanized transgenic mouse lines with hybrid HLA I class molecules on the cell surface, which correspond to the human allelic variants HLA-A*02:01, HLA-B*07:02, and HLA-C*07:02, were developed at the Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia. In this article, we present experimental data on quantitative determination of β2-microglobulin protein and HLA by the “sandwich” ELISA method in mice with different alleles of HLA I class genes. The results obtained confirm the presence of target functional proteins (transgenicity) in humanized transgenic mice, which is consistent with our previous data obtained when determining the primary sequence of the transgene using Sanger sequencing. We also discuss the scientific and practical significance of such biomodels, as well as the scope of their application.
O PENSAR FILOSÓFICO
Authors: Oliveira Valeria Silva de 
Published: July 2024

Visando mapear os rastros do pensamento ocidental como o conhecemos hoje e as transformações do pensar filosófico que conduziram à nova sensibilidade helenística de então, o presente trabalho encontra-se dividido em duas partes. A primeira disserta brevemente sobre a importância dos pré-socráticos, da Sofística, de Sócrates e Platão para a história do pensamento filosófico ocidental. A segunda parte reflete brevemente sobre o processo de transição da era clássica para a era helenística e as contribuições de Epicuro enquanto fundador de uma das grandes escolas helenísticas da época. Por fim, observa-se que enquanto herdeiros desses pensamentos, conseguimos compreendê-los, conforme sugere Vernant (1999). Destaco a importância de, a partir da compreensão desses pensamentos, reconhecermos o potencial para possibilidade de surgimento de novas formas de pensar, ser e existir no presente e no/para o futuro.
Role of the mesenchymal stromal cells in bone marrow failure of Fanconi Anemia patients
Authors: Zubicaray Josune , Ivanova Maria , Iriondo June , García Martínez Jorge , Muñoz-Viana Rafael , Abad Lorea , García-García Lorena , González de Pablo Jesús , Gálvez Eva , Sebastián Elena , Ramírez Manuel , Madero Luis , Díaz Miguel Ángel , González-Murillo África , Sevilla Julián 
Published: July 2024

IntroductionFanconi anemia (FA) is an inherited disorder characterized by bone marrow failure, congenital malformations, and predisposition to malignancies. Alterations in hematopoietic stem cells (HSC) have been reported, but little is known regarding the bone marrow (BM) stroma. Thus, the characterization of Mesenchymal Stromal Cells (MSC) would help to elucidate their involvement in the BM failure.MethodsWe characterized MSCs of 28 FA patients (FA-MSC) before and after treatment (hematopoietic stem cell transplantation, HSCT; or gene therapy, GT). Phenotypic and functional properties were analyzed and compared with MSCs expanded from 26 healthy donors (HD-MSCs). FA-MSCs were genetically characterized through, mitomycin C-test and chimerism analysis. Furthermore, RNA-seq profiling was used to identify dysregulated metabolic pathways.ResultsOverall, FA-MSC had the same phenotypic and functional characteristics as HD-MSC. Of note, MSC-GT had a lower clonogenic efficiency. These findings were not confirmed in the whole FA patients’ cohort. Transcriptomic profiling identified dysregulation in HSC self-maintenance pathways in FA-MSC (HOX), and was confirmed by real-time quantitative polymerase chain reaction (RT-qPCR).DiscussionOur study provides a comprehensive characterization of FA-MSCs, including for the first time MSC-GT and constitutes the largest series published to date. Interestingly, transcript profiling revealed dysregulation of metabolic pathways related to HSC self-maintenance. Taken together, our results or findings provide new insights into the pathophysiology of the disease, although whether these niche defects are involved in the hematopoietic defects seen of FA deserves further investigation.
Water determines the intramolecular dynamics of proteins. En example of bovine serum albumin
Authors: Penkov Nikita V. 
Published: July 2024

In this work, the terahertz time-domain spectroscopy method analyzed solutions of bovine serum albumin (BSA) in two high concentrations (50 and 334 mg/mL) at three pH values (2.5, 6.5, 8.5) and the same solvents without protein, at 25°C. The spectra of dry BSA were also recorded. For the first time, a method for determining the complex dielectric permittivity of protein molecules in aqueous solutions, without the dielectric contribution of the aqueous phase, is proposed. It is shown that the dielectric permittivity of dissolved and dry BSA (lyophilized, in the native conformation) differ significantly in the terahertz frequency range. These differences are small near 70 cm−1, but they increase greatly with decreasing frequency. It was found that the dielectric losses of protein molecules in solution are close to the dielectric losses of the aqueous environment, which in this frequency range are determined by intermolecular relaxation processes of water. Since dielectric losses are directly related to molecular dynamics, this fact shows that the intramolecular dynamics of the protein completely adjusts to the intermolecular dynamics of the aqueous environment. It also indicates that the native conformation does not determine all the fundamental characteristics of a protein molecule, in particular, it does not determine the dynamics of the protein, which significantly depends on the water environment.
Body surface mapping of P-waves in sinus rhythm to predict recurrence following cardioversion for atrial fibrillation
Authors: Antoun Ibrahim , Li Xin , Kotb Ahmed , Barker Joseph , Mavilakandy Akash , Koev Ivelin , Vali Zakariyya , Somani Riyaz , Ng G. André 
Published: July 2024

BackgroundDirect current cardioversion (DCCV) is used as elective and emergency rhythm control for atrial fibrillation (AF). We aimed to explore the role of P-wave parameters measured during sinus rhythm using body surface mapping (BSM) in predicting successful DCCV for persistent atrial fibrillation (persAF) at 12 months.MethodsThis case–control study included 56 males &gt;18 years old who underwent DCCV for persAF. P-wave parameter collection after DCCV for AF was done using 128 unipolar leads. A band-pass filter of 1–50 Hz was utilised. Corrected P-wave duration (PWDc), P-wave amplitude, and P-wave dispersion were measured to predict 12-month outcomes and time of recurrence.ResultsThe mean age was 64 ± 4 years, and 23 patients (44%) were on amiodarone. The 12-month success rate was 44% (n = 23), while the rest reverted to AF after 2.6 ± 0.4 months. The parameters were comparable between successful and failed DCCV in the entire cohort and patients not on amiodarone. In patients on amiodarone, patients with failed arms had higher PWDc than those with successful arms (188 vs. 150 ms, P = 0.04). Receiver operator characteristic curve analysis for PWDc in the amiodarone cohort showed an area under the curve (AUC) of 0.75 and P = 0.049. A recurrence cut-off &gt;161 ms had a sensitivity of 69% and a specificity of 100%, with a hazard ratio of 10.7, P = 0.004. The parameters were not predictive of the time of recurrence.ConclusionIn patients on amiodarone, increased PWDc measured using BSM was associated with higher AF recurrence at 12 months following DCCV for persAF.
Identification and validation of CCL5 as a key gene in HIV infection and pulmonary arterial hypertension
Authors: Yang Mengyue , Bi Wen , Zhang Zhijie 
Published: July 2024

BackgroundThe relationship between human immunodeficiency virus (HIV) infection and pulmonary arterial hypertension (PAH) has garnered significant scrutiny. Individuals with HIV infection have a higher risk of developing PAH. However, the specific mechanism of HIV-associated PAH remains unclear. Our study aims at investigating the shared biomarkers in HIV infection and PAH and predicting the potential therapeutic target for HIV-associated PAH.MethodsData for HIV infection and PAH were downloaded from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) analysis was performed to detect shared genes in HIV infection and PAH. Enrichment analysis was conducted to identify the function of common DEGs. Protein-protein interaction (PPI) analysis was used to detect key genes. These crucial genes were subsequently verified by RT-qPCR. Finally, candidate drugs were identified by using the Drug Signatures Database (DSigDB).ResultsNineteen common DEGs were identified in HIV infection and PAH. Enrichment analysis exhibited that the functions of these genes were mainly enriched in inflammatory responses, mainly including cellular immunity and interaction between viral proteins and cytokines. By constructing PPI networks, we identified the key gene CC-type chemokine ligand 5 (CCL5), and we verified that CCL5 was highly expressed in hypoxia induced human pulmonary artery endothelial cells (hPAECs) and human pulmonary artery smooth muscle cells (hPASMCs). In addition, we predicted 10 potential drugs targeting CCL5 by Autodock Vina.ConclusionThis study revealed that CCL5 might be a common biomarker of HIV infection and PAH and provided a new therapeutic target for HIV-associated PAH. However, further clinical validation is still indispensable.
Efficacy and safety of catheter ablation for atrial fibrillation in patients with heart failure with preserved ejection fraction: a systematic review and meta-analysis
Authors: Chen Xiaomei , Zhang Xuge , Fang Xiang , Feng Shenghong 
Published: July 2024

BackgroundCatheter ablation (CA) effectively treats atrial fibrillation (AF) in heart failure (HF) with reduced ejection fraction (HFrEF), improving clinical outcomes. However, its benefits for AF patients with heart failure with preserved ejection fraction (HFpEF) are still unclear.MethodsWe systematically searched PubMed, Embase, Web of Science, the Cochrane Library, and Scopus for studies investigating outcomes of CA in AF patients with HFpEF. Efficacy indicators included freedom from AF and antiarrhythmic drugs (AAD) free AF elimination. Safety indicators comprised total complications, HF admission, all-cause admission, and all-cause mortality. Sixteen studies with 20,796 patients included in our research.ResultsThe comprehensive analysis demonstrated that, when comparing CA with medical therapy in HFpEF, no significant differences were observed in terms of HF admissions, all-cause admissions, and all-cause mortality [(OR: 0.42; 95% CI: 0.12–1.51, P = 0.19), (HR: 0.78; 95% CI: 0.48–1.27, P = 0.31), and (OR: 1.10; 95% CI: 0.83–1.44, P = 0.51)], while freedom from AF was significantly higher in CA (OR: 5.88; 95% CI: 2.99–11.54, P &lt; 0.00001). Compared with HFrEF, CA in HFpEF showed similar rates of freedom from AF, AAD-free AF elimination, total complications, and all-cause admission were similar [(OR:0.91; 95% CI: 0.71,1.17, P =0.47), (OR: 0.97; 95% CI: 0.50–1.86, P = 0.93), (OR: 1.27; 95% CI: 0.47–3.41, P = 0.64), (OR: 1.11; 95% CI: 0.72, 1.73; P = 0.63)]. However, CA in HFpEF was associated with lower rates of HF admission and all-cause mortality [(OR: 0.35; 95% CI: 0.20, 0.60; P = 0.0002), (OR: 0.40; 95% CI: 0.18, 0.85; P = 0.02)]. Compared with patients without HF, CA in HFpEF patients exhibited lower rates of AAD-free AF elimination (OR: 0.48; 95% CI: 0.30, 0.75; P = 0.001). However, their rates of freedom from AF and total complications were similar [(OR: 0.70; 95% CI: 0.48, 1.02; P = 0.06), (OR: 0.60; 95% CI: 0.19, 1.90; P = 0.38)].ConclusionThis meta-analysis conducted provided a comprehensive evaluation of the efficacy and safety of CA in patients with AF and HFpEF. The results suggest that CA may represent a valuable treatment strategy for patients with AF and HFpEF.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier (CRD42024514169).
Revealing the characteristics of SETD2-mutated clear cell renal cell carcinoma through tumor heterogeneity analysis
Authors: Peng Shansen , Xie Zhouzhou , Jiang Huiming , Zhang Guihao , Chen Nanhui 
Published: July 2024

BackgroundRenal cell carcinoma (RCC) is the most prevalent type of malignant kidney tumor in adults, with clear cell renal cell carcinoma (ccRCC) comprising about 75% of all cases. The SETD2 gene, which is involved in the modification of histone proteins, is often found to have alterations in ccRCC. Yet, our understanding of how these SETD2 mutations affect ccRCC characteristics and behavior within the tumor microenvironment is still not fully understood.MethodsWe conducted a detailed analysis of single-cell RNA sequencing (scRNA-seq) data from ccRCC. First, the data was preprocessed using the Python package, “scanpy.” High variability genes were pinpointed through Pearson’s correlation coefficient. Dimensionality reduction and clustering identification were performed using Principal Component Analysis (PCA) and the Leiden algorithm. Malignant cell identification was conducted with the “InferCNV” R package, while cell trajectories and intercellular communication were depicted using the Python packages “VIA” and “cellphoneDB.” We then employed the R package “Deseq2” to determine differentially expressed genes (DEGs) between groups. Using high-dimensional weighted gene correlation network analysis (hdWGCNA), co-expression modules were identified. We intersected these modules with DEGs to establish prognostic models through univariate Cox and the least absolute shrinkage and selection operator (LASSO) method.ResultsWe identified 69 and 53 distinctive cell clusters, respectively. These were classified further into 12 unique cell types. This analysis highlighted the presence of an abnormal tumor sub-cluster (MT + group), identified by high mitochondrial-encoded protein gene expression and an indication of unfavorable prognosis. Investigation of cellular interactions spotlighted significant interactions between the MT + group and endothelial cells, macrophaes. In addition, we developed a prognostic model based on six characteristic genes. Notably, risk scores derived from these genes correlated significantly with various clinical features. Finally, a nomogram model was established to facilitate more accurate outcome prediction, incorporating four independent risk factors.ConclusionOur findings provide insight into the crucial transcriptomic characteristics of ccRCC associated with SETD2 mutation. We discovered that this mutation-induced subcluster could stimulate M2 polarization in macrophages, suggesting a heightened propensity for metastasis. Moreover, our prognostic model demonstrated effectiveness in forecasting overall survival for ccRCC patients, thus presenting a valuable clinical tool.
Association between sarcopenia and hemoglobin level: a systematic review and meta-analysis
Authors: Wang Hui , Lin Ping 
Published: July 2024

BackgroundSarcopenia is a disease characterized by decreased skeletal muscle mass and function in elderly individuals. Decreased hemoglobin levels is a marker of anemia. According to reports, there may be an association between anemia and sarcopenia, but research is inconsistent. Therefore, this meta-analysis aims to explore the association between sarcopenia and low hemoglobin levels.MethodsWe searched PubMed, Embase, the Cochrane Library, Web of Science, Ovid, China National Knowledge Infrastructure (CNKI), and Wan Fang databases until September 2022. The present study included cross-sectional and case-control studies regarding low hemoglobin levels and sarcopenia. The studies were selected using inclusion and exclusion criteria. Studies were meta-analyzed by Review Manager 5.4 and Stata 16.0. We performed the heterogeneity test using the I2 test. Subgroup analysis was carried out to explore the cause of heterogeneity. Egger test was used to evaluate publication bias.ResultsOut of 1,550 initial studies, 16 studies were meta-analyzed. Sarcopenia participants had significantly lower levels of hemoglobin than controls (MD = −0.53, 95% CI: −0.68 to −0.37, p &lt; 0.001). Subgroup analysis, performed in China population reported lower hemoglobin levels in the sarcopenia population (MD = −0.49, 95% CI: −0.65 to −0.33, p &lt; 0.001). And sarcopenia based on AWGS criteria reported lower hemoglobin levels (MD = −0.49, 95% CI: −0.65 to −0.33, p &lt; 0.001). Among the population from hospitals and communities, patients with sarcopenia have lower hemoglobin levels.ConclusionOur meta-analysis found evidence that sarcopenia is associated with low hemoglobin levels. However, further large-scale prospective studies should be conducted in the future to further confirm our conclusions.Systematic review registrationPROSPERO, CDR42024532252.