bluuebunny/crossref_metadata_2025_split huggingface dataset:
Budidaya Cacing Sutra (Tubifex sp) Pada Media Berbeda Dengan Metode Semi Closed Resirculation System (SCRS)
Authors: Masaniku Nizam , Astuti Ida , Ningsih Asniati 
Published: August 2024

Tubifex sp atau Cacing Sutera merupakan salah satu jenis pakan alami yang disukai larva ikan karena mengandung nutrisi yang tinggi untuk pertumbuhan larva ikan. Penelitian ini bertujuan untuk mengetahui jumlah biomassa Cacing Sutra pada media berbeda dengan metode sistem resirkulasi terhadap pertumbuhan Cacing Sutra. Penelitian ini dilaksanakan pada bulan Maret sampai April 2018 yang bertempat di Unit Pembenihan Rakyat Daseng Kota Gorontalo. Penelitian ini menggunakan metode eksperimen rancangan acak lengkap (RAL) satu faktor dengan 3 taraf perlakuan dan 3 kali pengulangan. Perlakuan pada penelitian ini adalah tanpa menggunakan pupuk (Kontrol) A, dengan pupuk kotoran ayam 500 g/m2 (B), pupuk kotoran sapi 500 g/m2 (C), dan pupuk kotoran kambing 500 g/m2 (D). Hasil penelitian ini menunjukkan bahwa perlakuan B signifikan terhadap pertumbuhan bobot biomassa Cacing Sutra dengan berat 111.8 g/m2. Hasil penelitian ini dapat disimpulkan bahwa pemberian pupuk kotoran ayam dapat mempengaruhi pertumbuhan Cacing Sutra.
STUDI PENGARUH KEHILANGAN FRAKSI AGREGAT HALUS PADA SPESIFIKASI REAM TERHADAP CAMPURAN ASPAL PORUS
Authors: Muslimin Eviliona 
Published: August 2024

This study aims to determine the effect of changing the gradation of the Road Engineering Association Of Malaysia (REAM) method with the loss of the fine fraction of REAM in the porous asphalt mixture using waste oil as an asbuton rejuvenator on the characteristics of marshall with the hot and cold mixture method. The results showed that Asbuton Hot Mixed Overlay Cold as Hollow Asphalt can be stored first and compacted at a temperature of 25°C. The marshall characteristic value is only the VIM value that meets the specifications for 2 variations of gradation. Penelitian ini bertujuan mengetahui pengaruh perubahan penggunaan gradasi metode Road Engineering Association Of Malaysia (REAM ) dengan kehilangan fraksi halus REAM pada campuran Aspal porus menggunakan limbah oli sebagai peremaja asbuton terhadap karakteristik marshall dengan metode campuran panas hampar dingin. Hasil penelitian menunjukkan bahwa Asbuton Campuran Panas Hampar Dingin sebagai Aspal berongga dapat disimpan terlebih dahulu dan dipadatkan pada suhu 25°C, Nilai karakeristik marshall hanya nilai VIM yang memenuhi spesifikasi terhadap 2 variasi gradasi.
A Design-Oriented Framework for Interactive Digital Narrative (IDN) in Virtual Reality (Focus on Branching Narrative and Agency)
Authors: Soltani Samira 
Published: August 2024

<p>Finding a design-oriented framework for interactive digital narrative (IDN) in virtual reality (VR) focusing on branching narrative and agency is the main goal of this research. Incorporating a practice-based research methodology, this study analyzed three virtual reality projects focusing on agency and three products from different media (print, movie, VR experience) focusing on branching narratives. The extracted framework comprises six sections: the interactor, actions, decisions, feedback and acknowledgment, VR features, and Accessibility. This design-oriented framework is applied to a narrative with a fundamental choices branch at the first scene, which makes the interactor's experience different. As explained in the design-oriented framework, all of the branches in this narrative are not determinative for the storylines, and sometimes they just play a vital role in the branching experiences. </p>
Ripple: A Wearable Environment : Exploring Subspace Through an Experimental, Large Scale Textile Installation
Authors: Armenti Deanna 
Published: August 2024

<p><em>Ripple: A Wearable Environment</em> is an experimental large-scale textile installation that explores subspace. Within the kink community, subspace has been explained as a meditative, dream-like state that feels like floating in water which is experienced when engaging in BDSM scenes. However, since most submissives experience subspace in a multitude of ways, the liminal temporality of subspace has remained a vague and generalized phenomenon. Experimental practice-based research techniques are utilized to dive deeper into subspace by exploring the flow of subspace and fetish items as talismanic sacred objects through an embodied lens. </p>
<p><em>Ripple</em> pushes against the misconceptions of the queer, kink experience through redefining not only fetish fashion, but also what sexy is and can be. Throughout the project the use of unconventional colour palettes, loose knit and free form crochet creates an "anti-aesthetic" to the well-known styles found in fetish fashion. The intention is not to make queer kink and fetish fashion more palatable to the masses, but rather to create an embodied wearable that speaks more genuinely to the emotional and internal experience of submissive kinksters.</p>
Lead Oxide Modified Graphite Electrodes for Electrochemical Degradation of Congo Red Dye in Aqueous Solution
Authors: Ohanele Emmanuela , Oguzie Kanayo , Ezema Fabian , Oguzie Emeka 
Published: August 2024

Congo red (CR) dye in aqueous solution was decolorized by an electrolysis process using graphite (G) and lead dioxide modified graphite (G/PbO2) as anode materials in a two-electrode batch reactor. The electrodeposited lead dioxide film was characterized by scanning electron microscopy coupled with energy dispersive spectroscopy (SEM-EDS) and atomic force microscopy (AFM). Comparative performance assessment of the anode materials under different process parameters reveals that the lead dioxide film improved the electrocatalytic effect of the modified electrode. The adjustment of the deposition bath pH from 1.5 to 3 resulted in the formation of uniform agglomeration and disappearance of particulates, while addition of sodium dodecyl sulphate (SDS) gave better adhesion of film to substrate. The degradation rate (DR) observed for the G/PbO2 (1.0 × 10−2 cm2) was higher than that of the unmodified electrode (0.87 × 10−2 cm2). Increase in applied voltage from 25 to 30 V at 23 mA/mm2 improved the degradation efficiency (DE) from 84.7% to 91.32% for graphite and from 96.09% to 99.98% for G/PbO2, with 0.5 M KCl. The prime degradation time of 45 min was recorded for graphite anode which reduced to 30 min for G/PbO2 anode. CR degraded to compounds with smaller molecular weight and better stability as observed from GC-MS analysis and computational total energy study, respectively. The modification of the graphite electrode surface by electrodepositing PbO2 film improved the DE and the prime reaction time. These findings present significant suggestions for the design of advanced electrodeposition and electrocatalytic systems for wastewater treatment applications.
FORECASTING SALES USING SARIMA MODELS AT THE SINAR PAGI BUILDING MATERIALS STORE
Authors: Aminullah Ahmad Adiib , Idhom Mohammad , Saputra Wahyu Syaifullah Jauharis 
Published: August 2024

Sinar Pagi Building Materials Store faces the challenge of maintaining optimal stock levels of goods to avoid excess and understock, which affects customer satisfaction and operational efficiency. This study applies the Seasonal Autoregressive Integrated Moving Average (SARIMA) method to forecast sales in the store. Leveraging its ability to model seasonal patterns on historical sales data, various SARIMA models were analyzed and compared using the Akaike Information Criterion (AIC) and Root Mean Square Error (RMSE). The dataset is divided by a 95:5 ratio into training and testing sets for robust evaluation. The results show that the SARIMA model with SARIMA notation (p,d,q)(P,D,Q has the best model value of (1,0,0) . This model is the most suitable model based on the lowest AIC value of 1245 and the lowest RMSE of 7,95 compared to other SARIMA models after model identification using the model looping test. For other models such as model (1,0,1) and (0,0,1) , the AIC and RMSE values are greater, namely model (1,0,1) with AIC 1246 and RMSE of 8,05, while model (0,0,1) gets an AIC of 1252 and an AIC of 8,15 .The lower the AIC value, the better the model and the lower the RMSE value, the better the model. This shows a superior balance between model complexity and prediction accuracy. The model manages to capture seasonal patterns in sales data, providing a pretty good prediction framework. This study shows that the SARIMA (1,0,0) model is effective in the accuracy of the sales forecasting process so that Sinar Pagi Building Materials Store can make more reliable sales predictions, which can help in inventory planning and marketing strategies
Propagación de tacaco (Sechium tacaco) mediante esquejes: efecto del ácido indol butírico
Authors: Muñoz-López Karen M. , Monge-Pérez José E. 
Published: August 2024

Objetivos: evaluar el efecto de varias dosis de ácido indol butírico sobre el enraizamiento y crecimiento de esquejes de tacaco (Sechium tacaco), en la fase de establecimiento en invernadero. Metodología: se utilizaron esquejes de brote secundario con al menos tres nudos, procedentes de fincas de productores de los cantones de Alvarado y Paraíso, Cartago, Costa Rica. Cuatro dosis de ácido indol butírico fueron evaluadas: 0; 0.15; 0.30; y 0.60mg/esqueje. Los esquejes fueron sembrados en macetas, con turba como sustrato, y se mantuvieron en invernadero por 22 días. Resultados: Diferencias significativas fueron halladas para las variables peso seco de raíz, porcentaje de enraizamiento, y porcentaje de supervivencia. No hubo diferencias significativas para el cambio de altura de la planta. Los tratamientos 0.30 y 0.60mg/esqueje de ácido indol butírico lograron valores de enraizamiento (51-63%) y peso seco de raíz (27.94-39.51mg/planta) significativamente superiores, con respecto al tratamiento sin ácido indol butírico (5% y 0.20mg/planta, respectivamente). Por otra parte, el tratamiento 0.60mg/esqueje de ácido indol butírico mostró un porcentaje de supervivencia (58%) significativamente inferior, con respecto a los demás tratamientos (84-90%). Conclusiones: El mejor tratamiento para la propagación de tacaco mediante esquejes fue 0.30mg/esqueje de ácido indol butírico, correspondiente a 10,000ppm. La información generada en este estudio contribuye de manera significativa en la elaboración de un protocolo de propagación asexual de tacaco mediante esquejes, a partir de brotes secundarios de plantas madre. Este trabajo constituye el primer informe publicado sobre el efecto de ácido indol butírico sobre esquejes de Sechium tacaco.
Design and synthesis of new 1,2,4-oxadiazole/quinazoline-4-one hybrids with antiproliferative activity as multitargeted inhibitors
Authors: Mohamed Amira M. , Abou-Ghadir Ola M. F. , Mostafa Yaser A. , Dahlous Kholood A. , Bräse Stefan , Youssif Bahaa G. M. 
Published: August 2024

IntroductionThe combination of BRAF and tyrosine kinase (TK) inhibitors has been demonstrated to be highly effective in inhibiting tumor development and is an approach for overcoming resistance in clinical trials. Accordingly, a novel series of 1,2,4-oxadiazole/quinazoline-4-one hybrids was developed as antiproliferative multitargeted inhibitors.MethodsThe structures of the newly synthesized compounds 9a-o were validated using IR, NMR, MS, and elemental techniques. 9a–o were tested as antiproliferative agents.Results and DiscussionThe results showed that the majority of the tested compounds showed significant antiproliferative action with 9b, 9c, 9h, 9k, and 9l being the most potent. Compounds 9b, 9c, 9h, 9k, and 9l were tested as EGFR and BRAFV600E inhibitors. These in vitro tests revealed that compounds 9b, 9c, and 9h are strong antiproliferative agents that may act as dual EGFR/BRAFV600E inhibitors. 9b, 9c, and 9h were further investigated for their inhibitory effect on mutant EGFR (EGFRT790M), and the results showed that the tested compounds had considerable inhibitory action. Cell cycle study and apoptosis detection demonstrated that compound 9b exhibits cell cycle arrest at the G2/M transition. Molecular docking simulations reveal the binding mechanism of the most active antiproliferative agents.
GH receptor polymorphisms guide second-line therapies to prevent acromegaly skeletal fragility: preliminary results of a pilot study
Authors: Chiloiro Sabrina , Costanza Flavia , Giampietro Antonella , Infante Amato , Mattogno Pier Paolo , Angelini Flavia , Gullì Consolato , Lauretti Liverana , Rigante Mario , Olivi Alessandro , De Marinis Laura , Doglietto Francesco , Bianchi Antonio , Pontecorvi Alfredo 
Published: August 2024

BackgroundSkeletal fragility is characterized by increased frequency of vertebral fractures (VFs) in acromegaly. Several trials were conducted to identify modifiable risk factors and predictors of VFs, with limited data on the prognostic role of GH receptor (GHR) isoforms. In this study, we investigated the potential role of GHR polymorphism on the occurrence of incidental VFs (i-VFs), in patients treated with second-line medical therapies.MethodsA longitudinal, retrospective, observational study was conducted on a cohort of 45 acromegalic patients not-responsive to first-generation somatostatin receptor ligands (fg-SRLs) and treated with GHR antagonist (Pegvisomant) or with the second-generation SRLs (Pasireotide long-acting release).ResultsSecond line treatments were Pegvisomant plus fg-SRLs in 26 patients and Pasireotide LAR in 19 patients. From the group treated with fg-SRLs+Peg-V, the fl-GHR isoform was identified in 18 patients (69.2%) and the d3-GHR isoform in 8 patients (30.8%). I-VFs arose exclusively in fl-GHR isoform carriers (p=0.039). From the group treated with Pasireotide LAR, the fl-GHR isoform was identified in 11 patients (57.9%), and the d3-GHR isoform in 8 patients (42.1%). I-VFs arose exclusively in d3-GHR isoform carriers (p=0.018). Patients with fl-GHR isoform had a higher risk for i-VFs if treated with fg-SRL+Peg-V (OR: 1.6 95%IC: 1.1-2.3, p=0.04), and a lower risk if treated with Pasi-LAR (OR: 0.26 IC95%: 0.11-0.66, p=0.038).ConclusionsOur data support a predictive role of the GHR isoforms for the occurrence of i-VFs in acromegalic patients treated with second-line drugs, tailored to the individual patient. The knowledge of the GHR polymorphism may facilitate the choice of second-line therapies, improving the therapeutic approach, in the context of personalized medicine.
Epidemiological characteristics of myopia among school-age children before, during, and after the COVID-19 pandemic: a cohort study in Shenzhen, China
Authors: Mu Jingfeng , Zhong Haoxi , Jiang Mingjie , Yang Weihua 
Published: August 2024

ObjectivesTo evaluate the epidemiological characteristics of myopia among school-aged children before, during, and after the coronavirus disease (COVID-19) pandemic.MethodsA total of 848,697 students aged 6–15 years from 786 primary and secondary schools in Shenzhen, China, were randomly selected as research subjects. We conducted annual myopia screenings from 2019 to 2022. 2019 was considered before the COVID-19 pandemic, 2020 as during the pandemic, and 2021 and 2022 as after the pandemic. Demographic characteristics, visual acuity, and spherical equivalent refraction (SE) were collected.ResultsDuring the 4-year follow-up period, the uncorrected visual acuity (UCVA) of the study subjects progressed following a trend of −0.18 ± 0.30D (−0.17 ± 0.29D for boys, −0.21 ± 0.32Dfor girls) (p &lt; 0.001). Those students who were in grade 4 aged 9–10 years at the baseline examination showed the greatest decline in visual acuity (0.23). The SE of the study subjects progressed following a trend of −1.00 ± 1.27D (−0.96 ± 1.25D for boys, −1.05 ± 1.31D for girls) (p &lt; 0.001). The students who were in grade 5 aged 10–11 years at the baseline examination showed the greatest decline in SE (1.15D ± 1.22, p &lt; 0.001). The prevalence of myopia (UCVA&lt;5.0 and SE &lt; –0.50D of any eye) increased by 28.2% (27.0% for boys and 29.8% for girls). Those students who were in grade 2 aged 7–8 years at the baseline examination showed the greatest increase in myopia prevalence (37.6%, p &lt; 0.001). During the COVID-19 pandemic, the subjects’ visual acuity and SE measurements decreased by −0.05 ± 0.19 (p &lt; 0.001) and − 0.36 ± 0.89D (p &lt; 0.001) respectively, and the prevalence of myopia increased by 11.3% (10.6% for boys and 12.2% for girls) (p &lt; 0.001). The 3-year cumulative incidence of myopia for non-myopic grade 1 aged 6–7 years students with baseline SE of ≥1.00D, ≥ 0.50D and &lt; 1.00D, ≥0D and &lt; 0.50D, and ≥ −0.50D and &lt; 0D were 6.8, 24.8, 39.0, and 48.1%, respectively.ConclusionDuring the COVID-19 pandemic, the SE of school-aged children showed myopic drift and decreased visual acuity. Myopia progressed faster among girls than among boys in the same grades. The risk of myopia among school-aged children persisted even after the home quarantine of the COVID-19 pandemic was lifted.
Application of Bacillus tequilensis for the control of gray mold caused by Botrytis cinerea in blueberry and mechanisms of action: inducing phenylpropanoid pathway metabolism
Authors: Du Qianjie , Li Raoyong , Liu Li , Chen Lin , Tang Junrong , Deng Jia , Wang Fang 
Published: August 2024

BackgroundBotrytis cinerea a blueberry gray mold, is one of the main diseases affecting postharvest storage, causing significant losses. Several studies have shown that Bacillus tequilensis can prevent the growth of plant pathogens by producing various antibacterial substances, and can induce plant resistance. However, research on the biological management of post-harvest gray mold in blueberries using B. tequilensis remains unclear.MethodsTo better control the postharvest gray mold of blueberry, the effects of B. tequilensis KXF6501 fermentation solution (YY) and KXF6501 cell-free supernatant (SQ) on the induction of disease resistance in blueberry fruits were studied using biochemical and transcriptomic analyses.ResultsWe found that YY controlled the conidial germination and mycelial growth of B. cinerea in vitro, followed by SQ. After 3 d of culture, the lesion diameter and incidence of gray mold in blueberry fruits inoculated with YY and SQ were smaller than those in the control group. Therefore, gray mold in blueberries was effectively controlled during the prevention period, and the control effect of YY was better than that of SQ. Transcription spectrum analysis of blueberry peel tissue showed that the YY- and SQ-induced phenylpropane metabolic pathways had more differentially expressed genes (DEGs) than other biological pathways. In addition, biochemical analyses showed that YY treatment effectively enhanced the activity of enzymes related to the phenylpropane pathway (phenylalanine ammonialyase [PAL], cinnamate 4-hydroxylase [C4H], 4-coumarate CoA ligase [4CL], and polyphenol oxidase [PPO]) and stimulated the synthesis of lignin, total phenols, and flavonoids, followed by SQ. Compared with the control, the YY and SQ treatments reduced the weight loss rate and better maintained the appearance and nutritional quality of the blueberry fruits.ConclusionOur findings suggest that B. tequilensis KXF6501 is potentially useful as a suitable bio-control agent in harvested blueberries.
The shared molecular mechanism of spinal cord injury and sarcopenia: a comprehensive genomics analysis
Authors: Wang Binyang , Yang Xu , Li Chuanxiong , Yang Rongxing , Sun Tong , Yin Yong 
Published: August 2024

IntroductionThe occurrence of Spinal cord injury (SCI) brings economic burden and social burden to individuals, families and society, and the complications after SCI greatly affect the rehabilitation and treatment of patients in the later stage.This study focused on the potential biomarkers that co-exist in SCI and sarcopenia, with the expectation to diagnose and prognose patients in the acute phase and rehabilitation phase using comprehensive data analysis.MethodsThe datasets used in this study were downloaded from Gene Expression Omnibus (GEO) database. Firstly, the datasets were analyzed with the “DEseq2” and “Limma” R package to identify differentially expressed genes (DEGs), which were then visualized using volcano plots. The SCI and sarcopenia DEGs that overlapped were used to construct a protein–protein interaction (PPI) network. Three algorithms were used to obtain a list of the top 10 hub genes. Next, validation of the hub genes was performed using three datasets. According to the results, the top hub genes were DCN, FSTL1, and COL12A1, which subsequently underwent were Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. We also assessed immune cell infiltration with the CIBERSORT algorithm to explore the immune cell landscape. The correlations between the hub genes and age and body mass index were investigated. To illustrate the biological mechanisms of the hub genes more clearly, a single-cell RNA-seq dataset was assessed to determine gene expression when muscle injury occurred. According to our analysis and the role in muscle, we chose the fibro/adipogenic progenitors (FAPs) cluster in the next step of the analysis. In the sub cluster analysis, we use the “Monocle” package to perform the trajectory analysis in different injury time points and different cell states.ResultsA total of 144 overlapped genes were obtained from two datasets. Following PPI network analysis and validation, we finally identified three hub-genes (DCN, FSTL1, and COL12A1), which were significantly altered in sarcopenic SCI patients both before and after rehabilitation training. The three hub genes were also significantly expressed in the FAPs clusters. Furthermore, following injury, the expression of the hub genes changed with the time points, changing in FAPs cluster.DiscussionOur study provides comprehensive insights into how muscle changes after SCI are associated with sarcopenia by moving from RNA-seq to RNA-SEQ, including Immune infiltration landscape, pesudotime change and so on. The three hub genes identified in this study could be used to distinguish the sarcopenia state at the genomic level. Additionally, they may also play a prognostic role in evaluating the efficiency of rehabilitation training.
Lung fibrosis in sarcoidosis. Is there a place for antifibrotics?
Authors: Bączek Karol , Piotrowski Wojciech Jerzy 
Published: August 2024

Sarcoidosis, an enigmatic disease with unknown etiology, is characterized by inflammation and the potential involvement of various organs, predominantly the lungs and intrathoracic lymph nodes. Non-caseating granulomas can resolve spontaneously in approximately 60% of cases within 2–3 years. However, sarcoidosis-related mortality has increased. Lung fibrosis, affecting up to 20% of sarcoidosis patients, stands out as a primary cause of mortality. Traditionally, fibrosis is viewed because of prolonged inflammation, necessitating anti-inflammatory treatment with systemic steroids, immunosuppressants, and anti-TNF agents to manage the disease. The recent introduction of antifibrotic drugs such as nintedanib and pirfenidone offers new avenues for treating fibrotic sarcoidosis. Nintedanib, effective in idiopathic pulmonary fibrosis (IPF) and systemic sclerosis-related interstitial lung disease (SSc-ILD), has shown promise in patients with various progressive fibrosing interstitial lung diseases (PF-ILD), including those with sarcoidosis. Pirfenidone, also effective in IPF, has demonstrated potential in managing fibrotic sarcoidosis, though results have been inconclusive due to limited participant numbers in studies. This review explores the theoretical and empirical evidence supporting the use of antifibrotics in sarcoidosis, weighing the benefits and drawbacks. While antifibrotics offer a potential therapeutic approach, further randomized controlled trials are essential to determine their efficacy in fibrotic sarcoidosis. Addressing fibrosis as a continuum of chronic inflammation, the role of antifibrotics in managing sarcoidosis remains an area requiring more in-depth research to improve patient outcomes and advance treatment paradigms.