bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Evolution of Soil Fertility Under Longterm Experiments in The Moldavian Plateau
Authors: Ailincăi Despina , Ailincăi C. 
Published: April 2013

Abstract
The paper presented the results of investigations concerning the influence of long-term fertilization (43 years) on some chemical characteristics of soil from the Moldavian Plateau, of wheat-maize rotation. The studies were performed on a typical cambic chernozem soil, prevailing in the Moldavian Plateau, and followed the dosing of fertilizers which ensures an efficient production increases and determines maintain or increase soil organic carbon content. Soil that has been placed experiences-clay loam, neutral to slightly acid reaction and a medium nutrient supply. On lands from the Moldavian Plateau, maintaining a good supply in mobile phosphorus for field crops (37-72 mg/kg) was done in case of the annual application of a rate of N100P100, and a very good supply in mobile phosphorus (69-78) and mobile potassium (over 200 mg/kg) was found at a rate of N100P100+60 t/ha manure. In wheat-maize rotation, applying a rate of N100P100 , for 43 years, has determined the pH decrease until the limit of moderately acid interval (5.1-5.8) and was maintained within the weakly acid interval (5.9-6.8) in case of the annual application of a rate of N50P50 + 60 t/ha manure. Soil organic carbon content, in wheat - maize rotation, decreased after 43 years of experiences from 19.8 to 16.3 g/kg in unfertilized variant and until 18.1 g/kg in case of the annual application of a rate of N100P100. In wheat - maize rotation, the significant increase in organic carbon content from soil has been registered at higher than N50P50 + 40 t/ha manure and at N100P100 + 60 t/ha manure. The determinations of mean annual amounts of nitrogen exported from soil by wheat (grains + straw) have shown that in wheat crop, its values varied according to fertilizers system, between 71.5 and 128.4 kg/ha. In maize crop, of the mean annual amounts of nitrogen exported from soil varied according to applied fertilizers, between 64.6 and 178.9 kg/ha.
Investigation of Avalanche Time and Carr’s Index of Poultry Litter Powder as Flowability Indices
Authors: Valaei I. , Hassan-Beygi S.R. , Kianmehr M.H. , Massah J. 
Published: April 2013

Abstract
The world’s dependence on chemical fertilizer as the primary source for enriching agricultural fields is continually increasing that cause nature pollution. This has led researchers to aggressively investigate renewable fertilizer resources, biomass, to produce organic crops and reduced wastage. Poultry litter is a bulk solid and biomass feed stocks. Flow behavior of bulk solid is a critical factor in designing and developing suitable equipments (e.g. pelletizing machine). The bulk density, tap density, Carr’s index and powder avalanche time technique were applied to evaluate the flow properties of poultry litter. The experiments were carried out at moisture content (10, 20 and 30% w.b.), particle size (0.3, 0.6 and 1.18 mm) for the bulk and tap densities as well as Carr’s index. In addition to the moisture content (10, 20 and 30 %w.b.) and particle size (0.3, 0.6 and 1.18 mm) the rotational speed of drum (0.5, 1 and1.5 rpm) were also investigated for the avalanche time. The results showed that with increasing moisture content Carr’s index increased significantly (P<0.01) in the ranges of 16.2% to 18.5% and with increasing particle size the Carr’s index decreased from 20.35% to 14.78%. The litter powder avalanche time (AT) increased significantly (P<0.01) with increasing moisture content and decreasing rotational speed and particle size. The bulk and tap densities of the litter powder was decreased with increasing moisture content and increasing the particle size. The bulk and tap densities of the driest and finest poultry litter sample were higher than other ones.
Phenolic Compounds in Merlot Wines Obtained Through Different Technologies in Iaşi Vineyard, Romania
Authors: Tudose-Sandu-Ville Şt. , Cotea V.V. , Colibaba Cintia , Nechita B. , Niculaua M. , Codreanu Maria 
Published: April 2013

Abstract
Obtaining red quality wines depends on the raw matter composition and also on the extraction technology, used in the processing of grape and must. Thus, two methods of maceration-fermentation on lees (classical and in rotating tanks), two thermal methods (thermomaceration and microwave maceration) and two methods rarely used industrially in wine-making (cryomaceration and ultrasound maceration) were experimented. Even in the years with less than favorable climatic conditions, from Merlot grape variety one obtained for the most part, legally speaking, quality wines (with the exception of microwave macerated and cryomacerated wines, which had only 21-22 g/L non-reducing extract. In regard to alcohol content, all obtained samples had more than 11% vol. The obtained Merlot wines were rich in glycerol (8-9 g/L), fact that favorably influenced their organoleptic traits. Total phenolic content had values between 1,97 and 2,86 g/L for the Merlot wines obtained through macerationfermentation and thermomaceration. Ultrasound maceration did not favor phenolic extraction from grape skins and the obtained wines were poor in anthocyans and tannins (0,94-1,1 g/L). In regard to the maceration technology used, free anthocyans were found in variable proportions in wines, between 77 and 91%. The sum of acylated anthocyans participation percentages was between 8,8 and 22,7%, and the ratio between the acetylated and cumarilated participation percentages registered small values, varietyspecific, between 1,1 and 2,8.
Phylogeny and fruit evolution in Menispermaceae
Authors: Wefferling Keir M. , Hoot Sara B. , Neves Susana S. 
Published: April 2013

• Premise of the study: This work surveys endocarp morphology of Menispermaceae in the context of a well‐supported molecular phylogeny. The study is important since menispermaceous endocarps appear often in the fossil record and indicate the presence of a wet forest ecosystem.• Methods: Three chloroplast regions were used to derive phylogenies for 53 genera and 60 species. Endocarps of 47 genera and 92 species were dissected and morphological characters scored. Photographs of key features are presented. We superimposed our morphological matrix onto the phylogeny to explore character evolution. A detailed key to fruits is presented, allowing identification of extant and fossil specimens to the level of clade or genus.• Key results: Menispermaceae consists of two major subfamilies: Tinosporoideae and Menispermoideae. Within Tinosporoideae, tribe Coscineae is basal. Within Menispermoideae, tribe Menispermeae is basal. Tinosporoideae consists mainly of taxa with apical style scars, bilateral curvature, subhemispherical condyles, and foliaceous cotyledons with divaricate or imbricate orientation. Menispermoideae consists almost entirely of taxa with basal or subbasal style scars, dorsoventral curvature, bilaterally and/or dorsoventrally compressed condyles, and subterete or fleshy cotyledons oriented dorsoventrally or laterally.• Conclusions: Several fruit characters differentiate major clades, and further synapomorphies are diagnostic of various subclades. Fruit characters that can be inferred as ancestral in the family are basal or subbasal stylar scars, endocarps with dorsoventral curvature, endocarp walls woody or bony, presence of a condyle, locule without ribs, sublateral vascular traces, presence of endosperm, and foliaceous or subterete cotyledons.
La France aux colonies : prise et reprise
Authors: Clarke Patrick D. 
Published: April 2013

Ce texte est une bête à deux têtes. Il est d’une part l’examen concis de La France aux colonies (1859), d’Edme Rameau de Saint-Père, maître ouvrage en Études acadiennes. Il est d’autre part une réflexion sur le croisement qu’engendre la rencontre du dictionnaire (littéraire ou autre) et de l’historiographie. Comme de raison, il se décline en deux parties. La première, une notice scientifique qui contient un ensemble d’indications sommaires, explicatives, semblable à celle qui chapeaute toute bonne étude en philologie linguistique. Puis, en second lieu, « l’entrée » elle-même, le texte sur lequel porte la « notice », un projet de publication dont la parution a été détournée. Il s’agit alors de faire d’une pierre deux coups. Primo, ajouter à nos connaissances au chapitre des lettres acadiennes; et, secundo, et par la même occasion, se faire, à soi, comme aux autres, un clin d’oeil.
Dipesh Chakrabarty et John M. Hobson sur l’eurocentrisme et la critique des relations internationales
Authors: Dufour Frédérick Guillaume , Turgeon Nancy 
Published: April 2013

Les auteurs présentent et analysent le projet théorique de Dipesh Chakrabarty – un important représentant des études postcoloniales – de « provincialiser » certains développements sociohistoriques de l’Europe. Ils se penchent également sur la critique de l’eurocentrisme de John M. Hobson, un important représentant de la sociologie historique néowébérienne des relations internationales. Après avoir présenté ces contributions aux théories postcoloniales et au virage anti-eurocentriste de certains sociologues néowébériens, les auteurs soulignent que ces théories ont tendance à s’élever contre une version dépassée du marxisme, ce qui les conduirait à négliger l’étude de l’articulation entre la modernité des relations internationales et l’émergence d’un ordre global capitaliste. Les auteurs concluent en défendant l’importance d’un détour par la théorie sociale classique pour l’examen des spécificités des arguments eurocentristes dans les relations internationales passées et contemporaines.
Association Between Cumulative Joint Loading From Occupational Activities and Knee Osteoarthritis
Authors: Ezzat Allison M. , Cibere Jolanda , Koehoorn Mieke , Li Linda C. 
Published: April 2013

ObjectiveTo determine the associations between cumulative occupational physical load (COPL) and 3 definitions of knee osteoarthritis (OA).MethodsCross‐sectional analyses were performed from 2 population‐based cohorts (n = 327). Eligible symptomatic participants were those with pain, aching, or discomfort in or around the knee on most days of a month at any time in the past and any pain in the past 12 months. Asymptomatic participants responded “no” to both knee pain questions. Self‐reported COPL was calculated over each participant's lifetime and then categorized into quarters (QCOPL). Radiographic OA (ROA) and symptomatic OA (SOA) were defined by Kellgren/Lawrence grade ≥2, with SOA also including pain. Magnetic resonance imaging (MRI) OA was defined using criteria by Hunter et al. Logistic regression, adjusted with population weights, examined the associations between QCOPL and each of ROA, SOA, and MRI‐OA after controlling for covariates and two‐way interactions.ResultsParticipants had a mean ± SD age of 58.5 ± 11.0 years and a mean ± SD body mass index of 26.3 ± 4.7 kg/m2. Of those, 109 (33.3%) had ROA, 102 (31.2%) had SOA, and 131 (40.1%) had MRI‐OA. Compared with QCOPL‐1, increased odds of ROA were found for QCOPL‐4 (odds ratio [OR] 3.15, 95% confidence interval [95% CI] 1.02–9.70) and QCOPL‐3 (OR 4.19, 95% CI 1.55–11.34). Statistically significant relationships were found in SOA (QCOPL‐4: OR 8.16, 95% CI 1.89–35.27; QCOPL‐3: OR 5.73, 95% CI 1.36–24.12) and MRI‐OA (QCOPL‐4: OR 9.54, 95% CI 2.65–34.27; QCOPL‐3: OR 9.04, 95% CI 2.65–30.88; QCOPL‐2: OR 7.18, 95% CI 2.17–23.70).ConclusionOccupational activity is associated with knee OA, with dose‐response relationships observed in SOA and MRI‐OA.
Dimensionality, Reliability, and Validity of the Revised Fibromyalgia Impact Questionnaire in Two Spanish Samples
Authors: Luciano Juan V. , Aguado Jaume , Serrano‐Blanco Antoni , Calandre Elena P. , Rodriguez‐Lopez Carmen M. 
Published: April 2013

ObjectiveThe present study attempted to fill a research gap by performing the first dimensionality analysis of the Revised Fibromyalgia Impact Questionnaire (FIQR) using exploratory and confirmatory techniques. A second objective was to report on the reliability and construct validity of the FIQR in Spanish patients.MethodsFIQR data from a sample of adult fibromyalgia patients (n = 113) were analyzed using principal components analysis (PCA). Subsequently, a set of confirmatory factor analyses (CFAs) was conducted in another sample (n = 179) to analyze the goodness of fit of various factor models. FIQR reliability was assessed by computing Cronbach's alpha and coefficient H. Construct validity was evaluated by comparing the FIQR scores of participants categorized by employment status.ResultsAccording to the PCA, the FIQR structure might be described as having 1 global factor of functional impairment. Although subsequent CFAs confirmed that 1 factor accounted for the greatest proportion of common variance in the FIQR items, a confirmatory bifactor analysis indicated that the items were multidimensional because of their simultaneous significant loading on specific factors. The Cronbach's alpha values of the FIQR domains were very good (>0.80) and the H estimate for the FIQR total score was excellent (0.93). Overall, the FIQR domains were able to distinguish between patients differing in employment status (working outside the home versus on sick leave).ConclusionOur results indicate that the Spanish version of the FIQR has a complex factor structure, has excellent reliability, and shows good construct validity.
Development and Validation of a New Disease Activity Score in 28 Joints–Based Treatment Response Criterion for Rheumatoid Arthritis
Authors: Behrens Frank , Tony Hans‐Peter , Alten Rieke , Kleinert Stefan , Scharbatke Eva C. , Köhm Michaela , Gnann Holger , Tams Johanna , Greger Gerd , Burkhardt Harald 
Published: April 2013

ObjectiveTo define a valid criterion for treatment response as assessed by the Disease Activity Score in 28 joints (DAS28) that exceeds random disease activity variations in patients with rheumatoid arthritis (RA).MethodsWe utilized anonymized data sets of RA patients from multiple rheumatology centers in Germany to identify patients with stable responses to conventional or biologic disease‐modifying antirheumatic drug (DMARD) therapy (discovery cohort). To evaluate fluctuations in DAS28 scores, we subjected patients' DAS28 scores at months 12, 18, and 24 to an analysis of variance model to establish a 1‐sided 95% confidence interval for normal fluctuations; this value was used to define the critical difference (DAS28‐dcrit) for individual changes from baseline. The DAS28‐dcrit value was then applied to analyses of therapeutic response in an adalimumab noninterventional study cohort.ResultsThe discovery cohort included 415 patients receiving stable treatment. Values for DAS28‐dcrit were comparable regardless of age, sex, disease activity, and class of therapy (DMARDs or biologic agents) and fell below 1.8 in all subgroups. We therefore conclude that DAS28 improvements of 1.8 or higher are outside the normal variation and represent a therapeutic response. When applied to data from the adalimumab noninterventional study (n = 1,874), a DAS28‐dcrit response was more robust over time than a European League Against Rheumatism response and was more closely correlated with improved functional capacity.ConclusionBased on our data, a DAS28‐dcrit value of 1.8 signifies a positive individual therapeutic response that exceeds the threshold of random fluctuation. The DAS28‐dcrit criterion may be useful in steering individual therapy and stratifying clinical trials.
ACTA2 mutation with childhood cardiovascular, autonomic and brain anomalies and severe outcome
Authors: Meuwissen Marije E.C. , Lequin Maarten H. , Bindels‐de Heus Karen , Bruggenwirth Hennie T. , Knapen Maarten F.C.M. , Dalinghaus Michiel , de Coo René , van Bever Yolande , Winkelman Beerend H.J. , Mancini Grazia M.S. 
Published: April 2013

AbstractThoracic aortic aneurysm and dissection (TAAD) are associated with connective tissue disorders like Marfan syndrome and Loeys–Dietz syndrome, caused by mutations in the fibrillin‐1, the TGFβ‐receptor 1‐ and ‐2 genes, the SMAD3 and TGFβ2 genes, but have also been ascribed to ACTA2 gene mutations in adults, spread throughout the gene. We report on a novel de novo c.535C > T in exon 6 leading to p.R179C aminoacid substitution in ACTA2 in a toddler girl with primary pulmonary hypertension, persistent ductus arteriosus, extensive cerebral white matter lesions, fixed dilated pupils, intestinal malrotation, and hypotonic bladder. Recently, de novo ACTA2 R179H substitutions have been associated with a similar phenotype and additional cerebral developmental defects including underdeveloped corpus callosum and vermis hypoplasia in a single patient. The patient here shows previously undescribed abnormal lobulation of the frontal lobes and position of the gyrus cinguli and rostral dysplasis of the corpus callosum; she died at the age of 3 years during surgery due to vascular fragility and rupture of the ductus arteriosus. Altogether these observations support a role of ACTA2 in brain development, especially related to the arginine at position 179. Although all previously reported patients with R179H substitution successfully underwent the same surgery at younger ages, the severe outcome of our patient warns against the devastating effects of the R179C substitution on vasculature. © 2013 Wiley Periodicals, Inc.
Expanding the phenotype of cardiovascular malformations in Adams–Oliver syndrome
Authors: Algaze Claudia , Esplin Edward D. , Lowenthal Alexander , Hudgins Louanne , Tacy Theresa Ann , Selamet Tierney Elif Seda 
Published: April 2013

AbstractWe describe a newborn with a phenotype consistent with Adams–Oliver syndrome and truncus arteriosus. Although cardiovascular malformations associated with this syndrome have been previously published in the literature, this is the first description of truncus arteriosus in a patient with Adams–Oliver syndrome. We review other reports of Adams–Oliver syndrome previously described with cardiovascular malformations, consider possible genetic and embryologic mechanisms, and emphasize the need for cardiology consultation when a diagnosis of Adams–Oliver syndrome is suspected in the differential diagnosis. © 2013 Wiley Periodicals, Inc.
Exome Analysis in Clinical Practice: Expanding the Phenotype of Bartsocas–Papas Syndrome
Authors: Gripp Karen W. , Ennis Sara , Napoli Joseph 
Published: April 2013

AbstractExome analysis has had a dramatic impact on genetic research. We present the application of such newly generated information to patient care. The patient was a female, born with normal growth parameters to nonconsanguineous parents after an uneventful pregnancy. She had bilateral cleft lip/palate and ankyloblepharon. Sparse hair, dysplastic nails and hypohidrosis were subsequently noted. With exception of speech related issues, her development was normal. A clinical diagnosis of ankyloblepharon–ectodermal defects‐cleft lip/palate or Hay–Wells syndrome resulted in TP63 sequence analysis. TP63 sequence and deletion/duplication analysis of all coding exons had a normal result, as did chromosome and SNP array analysis. Diagnostic exome analysis revealed a heterozygous nonsense mutation in KRT83 categorized as deleterious and associated with monilethrix. In addition, a homozygous missense variant of unknown clinical significance was reported in RIPK4. Using research based exome analysis, RIPK4 had just a few months prior been identified as pathogenic for Bartsocas–Papas syndrome. While the clinical diagnostic report implied the KRT83 mutation as a more likely cause for the patient's phenotype, clinical correlation, literature review and use of computerized mutation analysis programs allowed us to identify the homozygous RIPK4 (c.488G > A; p.Gly163Asp) mutation as the underlying pathogenic change. Consequently, we expand the phenotype of Bartsocas–Papas syndrome to an attenuated presentation resembling Hay–Wells syndrome, lacking lethality and pterygia. In contrast to the autosomal dominant Hay–Wells syndrome, Bartsocas–Papas syndrome is autosomal recessive, implying a 25% recurrence risk. © 2013 Wiley Periodicals, Inc.
Attitudes of African Americans Toward Return of Results From Exome and Whole Genome Sequencing
Authors: Yu Joon‐Ho , Crouch Julia , Jamal Seema M. , Tabor Holly K. , Bamshad Michael J. 
Published: April 2013

AbstractExome sequencing and whole genome sequencing (ES/WGS) present patients and research participants with the opportunity to benefit from a broad scope of genetic results of clinical and personal utility. Yet, this potential for benefit also risks disenfranchising populations such as African Americans (AAs) that are already underrepresented in genetic research and utilize genetic tests at lower rates than other populations. Understanding a diverse range of perspectives on consenting for ES/WGS and receiving ES/WGS results is necessary to ensure parity in genomic health care and research. We conducted a series of 13 focus groups (n = 76) to investigate if and how attitudes toward participation in ES/WGS research and return of results from ES/WGS differ between self‐described AAs and non‐AAs. The majority of both AAs and non‐AAs were willing to participate in WGS studies and receive individual genetic results, but the fraction not interested in either was higher in AAs. This is due in part to different expectations of health benefits from ES/WGS and how results should be managed. Our results underscore the need to develop and test culturally tailored strategies for returning ES/WGS results to AAs. © 2013 Wiley Periodicals, Inc.
Clinical Comparison of Overlapping Deletions of 19p13.3
Authors: Risheg Hiba , Pasion Romela , Sacharow Stephanie , Proud Virginia , Immken LaDonna , Schwartz Stuart , Tepperberg Jim H. , Papenhausen Peter , Tan Tiong Y. , Andrieux Joris , Plessis Ghislaine , Amor David J. , Keitges Elisabeth A. 
Published: April 2013

AbstractWe present three patients with overlapping interstitial deletions of 19p13.3 identified by high resolution SNP microarray analysis. All three had a similar phenotype characterized by intellectual disability or developmental delay, structural heart abnormalities, large head relative to height and weight or macrocephaly, and minor facial anomalies. Deletion sizes ranged from 792 Kb to 1.0 Mb and included a common region arr [hg19] 19p13.3 (3,814,392–4,136,989), containing eight genes: ZFR2, ATCAY, NMRK2, DAPK3, EEF2, PIAS4, ZBTB7A, MAP2K2, and two non‐coding RNA's MIR637 and SNORDU37. The patient phenotypes were compared with three previous single patient reports with similar interstitial 19p13.3 deletions and six additional patients from the DECIPHER and ISCA databases to determine if a common haploinsufficient phenotype for the region can be established. © 2013 Wiley Periodicals, Inc.
Practices and Policies of Clinical Exome Sequencing Providers: Analysis and Implications
Authors: Jamal Seema M. , Yu Joon‐Ho , Chong Jessica X. , Dent Karin M. , Conta Jessie H. , Tabor Holly K. , Bamshad Michael J. 
Published: April 2013

AbstractExome and whole genome sequencing (ES/WGS) offer potential advantages over traditional approaches to diagnostic genetic testing. Consequently, use of ES/WGS in clinical settings is rapidly becoming commonplace. Yet there are myriad moral, ethical, and perhaps legal implications attached to the use of ES and health care professionals and institutions will need to consider these implications in the context of the varied practices and policies of ES service providers. We developed “core elements” of content and procedures for informed consent, data sharing, and results management and a quantitative scale to assess the extent to which research protocols met the standards established by these core elements. We then used these tools to evaluate the practices and policies of each of the 6 U.S. CLIA‐certified labs offering clinical ES. Approaches toward informed consent, data sharing, and results return vary widely among ES providers as do the overall potential merits and disadvantages of each, and more importantly, the balance between the two. © 2013 Wiley Periodicals, Inc.