bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Nanocrystalline Iron Oxides, Composites, and Related Materials as a Platform for Electrochemical, Magnetic, and Chemical Biosensors
Authors: Urbanova Veronika , Magro Massimiliano , Gedanken Aharon , Baratella Davide , Vianello Fabio , Zboril Radek 
Published: January 2015

AbstractReview: 198 refs.
Enantioselective Degradation of Indoxacarb From Different Commercial Formulations Applied to Tea
Authors: Zhang Yuping , Hu Deyu , Meng Xingang , Shi Qingcai , Li Pei , Jin Linhong , Zhang Kankan , Song Baoan 
Published: January 2015

AbstractThe stereoselective degradation of indoxacarb enriched with (+)‐S‐indoxacarb (S/R:70/30) was investigated in three typical green teas. A convenient and precise chiral method was developed and validated for measuring indoxacarb enantiomers in green tea. The developed method was based on high‐performance liquid chromatography coupled with tandem mass spectrometry using a Chiralpak IC column. The stereoselective degradation of indoxacarb enantiomers showed that the (+)‐S‐enantiomer dissipated faster than the (−)‐R‐enantiomer in all three typical tea farms. However, no enantiomerization was observed after applying pure (+)‐S‐indoxacarb. Residues on tea plant of the active ingredient (+)‐S‐indoxacarb from suspension concentrate (SC) was more persistent than that from emulsifiable concentrate (EC). Chirality 27:262–267, 2015. © 2015 Wiley Periodicals, Inc.
Consensus guidelines on plasma cell myeloma minimal residual disease analysis and reporting
Authors: Arroz Maria , Came Neil , Lin Pei , Chen Weina , Yuan Constance , Lagoo Anand , Monreal Mariela , de Tute Ruth , Vergilio Jo‐Anne , Rawstron Andy C. , Paiva Bruno 
Published: January 2015

BackgroundMajor heterogeneity between laboratories in flow cytometry (FC) minimal residual disease (MRD) testing in multiple myeloma (MM) must be overcome. Cytometry societies such as the International Clinical Cytometry Society and the European Society for Clinical Cell Analysis recognize a strong need to establish minimally acceptable requirements and recommendations to perform such complex testing.MethodsA group of 11 flow cytometrists currently performing FC testing in MM using different instrumentation, panel designs (≥ 6‐color) and analysis software compared the procedures between their respective laboratories and reviewed the literature to propose a consensus guideline on flow‐MRD analysis and reporting in MM.Results/ConclusionConsensus guidelines support i) the use of minimum of five initial gating parameters (CD38, CD138, CD45, forward, and sideward light scatter) within the same aliquot for accurate identification of the total plasma cell compartment; ii) the analysis of potentially aberrant phenotypic markers and to report the antigen expression pattern on neoplastic plasma cells as being reduced, normal or increased, when compared to a normal reference plasma cell immunophenotype (obtained using the same instrument and parameters); and iii) the percentage of total bone marrow plasma cells plus the percentages of both normal and neoplastic plasma cells within the total bone marrow plasma cell compartment, and over total bone marrow cells. Consensus guidelines on minimal current and future MRD analyses should target a lower limit of detection of 0.001%, and ideally a limit of quantification of 0.001%, which requires at least 3 × 106and 5 × 106bone marrow cells to be measured, respectively. © 2015 International Clinical Cytometry Society
Abnormal immunophenotype of the T‐cell‐receptor beta Chain in follicular‐helper T cells of angioimmunoblastic T‐cell lymphoma
Authors: Mao Z. Jenny , Surowiecka Maria , Linden Michael A. , Singleton Timothy P. 
Published: January 2015

ObjectiveAnalysis for 24 variable regions of the T‐cell‐receptor beta chain by flow cytometry (Vbeta) is a new technique to detect clonal alpha‐beta T lymphocytes and is characteristically performed on peripheral blood. Angioimmunoblastic T‐cell lymphoma (AITL) has increased neoplastic follicular‐helper T cells (FHT), which often express CD10; but nonneoplastic, CD10‐positive T cells may be associated with reactive lymphadenopathy and with B‐cell lymphomas. This study documents the utility of Vbeta analysis of FHT in specimens of AITL from blood, from marrow, and from lymph nodes.MethodsThe electronic medical record in the flow cytometry laboratory was searched for specimens that were analyzed by flow cytometry for Vbeta and that were involved by AITL. Flow cytometry was performed for the following antigens: T‐cell‐associated proteins, CD10, CD56, CD94, CD161, killer‐cell immunoglobulin‐like receptors, alpha‐beta T‐cell receptor, gamma‐delta T‐cell receptor, and Vbeta.ResultsFive patients had six specimens of blood (two), of bone marrow (one), or of lymph nodes (three). Immunophenotypic aberrances were detected for antigens: CD2 (2/6), CD3 (6/6), CD4 (1/6), CD5 (1/6), CD7 (5/6), and CD45 (2/6). All abnormal T‐cell populations expressed CD4, and most expressed CD10 (5/6). Four specimens were clonally restricted for Vbeta. Two specimens lacked the alpha‐beta T‐cell receptor and Vbeta.ConclusionsVbeta analysis by flow cytometry can be used to detect clonal alpha‐beta FHT in AITL, which may be difficult to diagnose with early involvement. Abnormal Vbeta expression on CD10‐positive T cells confirms that FHT are the neoplastic cells. © 2014 International Clinical Cytometry Society
Circulating microRNAs in heart failure with reduced and preserved left ventricular ejection fraction
Authors: Wong Lee Lee , Armugam Arunmozhiarasi , Sepramaniam Sugunavathi , Karolina Dwi Setyowati , Lim Kai Ying , Lim Jia Yuen , Chong Jenny P. C. , Ng Jessica Y. X. , Chen Yei‐Tsung , Chan Michelle M. Y. , Chen Zhaojin , Yeo Poh Shuan D. , Ng Tze P. , Ling Lieng H. , Sim David , Leong Kui Toh G. , Ong Hean Y. , Jaufeerally Fazlur , Wong Raymond , Chai Ping , Low Adrian F. , Lam Carolyn S. P. , Jeyaseelan Kandiah , Richards Arthur Mark 
Published: January 2015

AbstractAimThe potential diagnostic utility of circulating microRNAs in heart failure (HF) or in distinguishing HF with reduced vs. preserved left ventricular ejection fraction (HFREF and HFPEF, respectively) is unclear. We sought to identify microRNAs suitable for diagnosis of HF and for distinguishing both HFREF and HFPEF from non‐HF controls and HFREF from HFPEF.Methods and resultsMicroRNA profiling performed on whole blood and corresponding plasma samples of 28 controls, 39 HFREF and 19 HFPEF identified 344 microRNAs to be dysregulated among the three groups. Further analysis using an independent cohort of 30 controls, 30 HFREF and 30 HFPEF, presented 12 microRNAs with diagnostic potential for one or both HF phenotypes. Of these, miR‐1233, ‐183‐3p, ‐190a, ‐193b‐3p, ‐193b‐5p, ‐211‐5p, ‐494, and ‐671‐5p distinguished HF from controls. Altered levels of miR‐125a‐5p, ‐183‐3p, ‐193b‐3p, ‐211‐5p, ‐494, ‐638, and ‐671‐5p were found in HFREF while levels of miR‐1233, ‐183‐3p, ‐190a, ‐193b‐3p, ‐193b‐5p, and ‐545‐5p distinguished HFPEF from controls. Four microRNAs (miR‐125a‐5p, ‐190a, ‐550a‐5p, and ‐638) distinguished HFREF from HFPEF. Selective microRNA panels showed stronger discriminative power than N‐terminal pro‐brain natriuretic peptide (NT‐proBNP). In addition, individual or multiple microRNAs used in combination with NT‐proBNP increased NT‐proBNP's discriminative performance, achieving perfect intergroup distinction. Pathway analysis revealed that the altered microRNAs expression was associated with several mechanisms of potential significance in HF.ConclusionsWe report specific microRNAs as potential biomarkers in distinguishing HF from non‐HF controls and in differentiating between HFREF and HFPEF.
Loop diuretic dose adjustments after a hospitalization for heart failure: insights from ASCEND‐HF
Authors: DeVore Adam D. , Hasselblad Vic , Mentz Robert J. , O'Connor Christopher M. , Armstrong Paul W. , McMurray John J. , Ezekowitz Justin A. , Tang W.H. Wilson , Starling Randall C. , Voors Adriaan A. , Califf Robert M. , Hernandez Adrian F. 
Published: January 2015

AbstractAimsLoop diuretics are a cornerstone of heart failure (HF) treatment, but data regarding diuretic dose adjustments after a HF hospitalization and the association with subsequent outcomes are limited. This study was therefore conducted to determine these factors.Methods and resultsWe analysed data from 6119 patients enrolled in ASCEND‐HF, examining the association between loop diuretic use at the time of discharge, compared with admission, and the composite outcome of 30‐day HF re‐hospitalization or all‐cause mortality. The majority of patients, 3921 (64%), were taking a loop diuretic on admission. At discharge, 3411 (56%) patients were prescribed higher doses compared with admission, including 1867 (31%) initiating daily outpatient diuretics; 1912 (31%) had no dose change and 795 (13%) were prescribed lower doses compared with admission. Initiation of an oral loop diuretic at discharge was independently associated with better 30‐day outcomes compared with no dose change [adjusted odds ratio (OR) 0.51, 95% confidence interval (CI) 0.37–0.68]. However, for patients that were already established on a loop diuretic prior to admission, change in the dose at discharge was not associated with improved outcomes compared with no dose change (adjusted OR 0.92, 95% CI 0.79–1.07).ConclusionsIn a large multinational clinical trial, 56% of patients hospitalized with HF were either initiated on a daily loop diuretic at discharge or discharged on higher doses compared with admission. In patients established on diuretics prior to hospitalization, we found no association between changes to chronic doses at discharge and improved outcomes, whereas initiation of loop diuretic therapy was associated with better outcomes compared with no dose change.
A Retrospective Likelihood Approach for Efficient Integration of Multiple Omics Factors in Case‐Control Association Studies
Authors: Balliu Brunilda , Tsonaka Roula , Boehringer Stefan , Houwing‐Duistermaat Jeanine 
Published: January 2015

ABSTRACTIntegrative omics, the joint analysis of outcome and multiple types of omics data, such as genomics, epigenomics, and transcriptomics data, constitute a promising approach for powerful and biologically relevant association studies. These studies often employ a case‐control design, and often include nonomics covariates, such as age and gender, that may modify the underlying omics risk factors. An open question is how to best integrate multiple omics and nonomics information to maximize statistical power in case‐control studies that ascertain individuals based on the phenotype. Recent work on integrative omics have used prospective approaches, modeling case‐control status conditional on omics, and nonomics risk factors. Compared to univariate approaches, jointly analyzing multiple risk factors with a prospective approach increases power in nonascertained cohorts. However, these prospective approaches often lose power in case‐control studies. In this article, we propose a novel statistical method for integrating multiple omics and nonomics factors in case‐control association studies. Our method is based on a retrospective likelihood function that models the joint distribution of omics and nonomics factors conditional on case‐control status. The new method provides accurate control of Type I error rate and has increased efficiency over prospective approaches in both simulated and real data.
Preparation of a hybrid monolithic stationary phase with allylsulfonate for the rapid and simultaneous separation of cations in capillary ion chromatography
Authors: Rahayu Aster , Lim Lee Wah , Takeuchi Toyohide 
Published: January 2015

A hybrid monolithic column with sulfonate functionality was successfully prepared for the simultaneous separation of common inorganic cations in ion‐exchange chromatographic mode through a simple and easy single‐step preparation method. The strong cation‐exchange moieties were provided directly from allylsulfonate, which worked as an organic monomer in the single‐step reaction. Inorganic cations (Li+, Na+, K+, NH4+, Cs+, Rb+, Mg2+, Ca2+, and Sr2+) were separated satisfactorily by using CuSO4 as the eluent with indirect UV detection. The allysulfonate hybrid monolith showed a better performance in terms of speed and pressure drop than the capillary packed column. The number of theoretical plates achieved was 19 017 plates/m (in the case of NH4+ as the analyte). The relative standard deviations (n = 6) of both retention time and peak height were less than 1.96% for all the analyte cations. The allysulfonate hybrid monolithic column was successfully applied for the rapid and simultaneous separation of inorganic cations in groundwater and the effluent of onsite domestic wastewater treatment system.
Bacteria Cellulose: Biopolymer from Gluconacetobacter Xylinus
Authors: Mathur Garima , Dua Aarushi , Das Anushuya Raj , Kaur Harleen , Kukal Samiksha , Sharma Parul , Goswami Navendu , Sahai Anshuman , Mathur Ashwani 
Published: January 2015

SummarySummary In the present study, production, characterization and optimization of bacterial cellulose (BC) from Gluconacetobacter xylinus MTCC 7795 has been investigated. Effect of various carbon sources and bioprocess parameters on bacterial growth rate and subsequent BC production were studied and optimized. Supplementation of the media with different carbon sources resulted in varying yield of bacterial cellulose. Variations in the arrangement of bacterial cellulose with variations in carbon source were analyzed using FT‐IR. Results have suggested that characteristic vibrational modes of BC produced were almost same in the typical fingerprint regions as in commercial cellulose.
Structural, Optical and Electrical Properties of Spray Pyrolysis Deposited CdS Films
Authors: Gunjal Shashikant D. , Khollam Yogesh B. , Arote Sandeep A. , Jadkar Sandesh R. , Shelke Pandit N. , Mohite Kakasaheb C. 
Published: January 2015

SummaryThe n‐type cadmium sulfide (CdS) is one of the most promising materials for hetero‐junction thin film solar cell applications due to its wide bandgap (2.42 eV). In present communication, the deposition of pure CdS films with hexagonal symmetry by using home‐built spray pyrolysis technique and aqueous solutions of CdCl2 and NH2.CS.NH2 as the precursor materials is reported. The films were deposited at the substrate temperatures of 200, 250 and 300 °C with UHP nitrogen as a carrier gas and characterized by using X‐ray diffraction (XRD), UV‐visible spectroscopy and Hall Effect measurement. The structural studies by using XRD showed that the resultant films have strong orientation of CdS crystallites along (101) with hexagonal structure (wurtzite type). The average crystallite size is found to be 17 nm. The UV‐visible spectroscopy studies of resultant films showed the optical transmittance equal to ≈50% and bandgap is found to be between 2.39 – 2.41 eV. The resistivity and average Hall coefficient are found to be 1.451 × 101 Ωcm and ‐ 1.557 × 103 cm3/C respectively. The resultant films are observed to be good to make a solar cell with CdS as a window layer.
Catechin and quercetin attenuate adipose inflammation in fructose‐fed rats and 3T3‐L1 adipocytes
Authors: Vazquez Prieto Marcela A. , Bettaieb Ahmed , Rodriguez Lanzi Cecilia , Soto Verónica C. , Perdicaro Diahann J. , Galmarini Claudio R. , Haj Fawaz G. , Miatello Roberto M. , Oteiza Patricia I. 
Published: January 2015

ScopeThis study evaluated the capacity of dietary catechin (C), quercetin (Q), and the combination of both (CQ), to attenuate adipose inflammation triggered by high fructose (HFr) consumption in rats and by tumor necrosis factor alpha (TNF‐α) in 3T3‐L1 adipocytes.Methods and resultsIn rats, HFr consumption for 6 wk caused dyslipidemia, insulin resistance, reduced plasma adiponectin, adiposity, and adipose tissue inflammation. Dietary supplementation with 20 mg/kg/day of C, Q, and CQ improved all these parameters. In 3T3‐L1 adipocytes, C and Q attenuated TNF‐α‐induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP‐1, resistin), and decreased adiponectin. The protective effects of C and Q on adipose inflammation are in part associated with their capacity to (i) decrease the activation of the mitogen‐activated kinases (MAPKs) JNK and p38; and (ii) prevent the downregulation of PPAR‐γ. In summary, C and Q, and to a larger extent the combination of both, attenuated adipose proinflammatory signaling cascades and regulated the balance of molecules that improve (adiponectin) or impair (TNF‐α, MCP‐1, resistin) insulin sensitivity.ConclusionTogether, these findings suggest that dietary Q and C may have potential benefits in mitigating MetS‐associated adipose inflammation, oxidative stress, and insulin resistance.
Enhanced bioavailability of lycopene when consumed as cis‐isomers from tangerine compared to red tomato juice, a randomized, cross‐over clinical trial
Authors: Cooperstone Jessica L. , Ralston Robin A. , Riedl Ken M. , Haufe Thomas C. , Schweiggert Ralf M. , King Samantha A. , Timmers Cynthia D. , Francis David M. , Lesinski Gregory B. , Clinton Steven K. , Schwartz Steven J. 
Published: January 2015

ScopeTangerine tomatoes (Solanum lycopersicum) are rich in tetra‐cis‐lycopene resulting from natural variation in carotenoid isomerase. Our objective was to compare the bioavailability of lycopene from tangerine to red tomato juice, and elucidate physical deposition forms of these isomers in tomatoes by light and electron microscopy.Methods and resultsFollowing a randomized cross‐over design, subjects (n = 11, 6 M/5 F) consumed two meals delivering 10 mg lycopene from tangerine (94% cis) or red tomato juice (10% cis). Blood was sampled over 12 h and triglyceride‐rich lipoprotein fractions of plasma were isolated and analyzed using HPLC‐DAD‐MS/MS. Lycopene was crystalline in red tomato chromoplasts and globular in tangerine tomatoes. With tangerine tomato juice we observed a marked 8.5‐fold increase in lycopene bioavailability compared to red tomato juice (p < 0.001). Fractional absorption was 47.70 ± 8.81% from tangerine and 4.98 ± 1.92% from red tomato juices. Large heterogeneity was observed among subjects.ConclusionLycopene is markedly more bioavailable from tangerine than from red tomato juice, consistent with a predominance of cis‐lycopene isomers and presence in chromoplasts in a lipid dissolved globular state. These results justify using tangerine tomatoes as a lycopene source in studies examining the potential health benefits of lycopene‐rich foods.
Nerve ultrasound in a case of multifocal motor neuropathy without conduction block
Authors: Pitarokoili Kalliopi , Gold Ralf , Yoon Min‐Suk 
Published: January 2015

ABSTRACTIntroduction: Multifocal nerve enlargements and ultrastructural changes either corresponding or not to sites of existing conduction blocks have been described in demyelinating polyneuropathies using multiple imaging techniques. Methods: Using the emerging technique of peripheral nerve ultrasonography we investigated the peripheral nerves of a patient with multifocal motor neuropathy (MMN) without conduction block. Results: In this case of MMN without conduction blocks we found multifocal nerve enlargements in the ultrasonography and electrodiagnostic signs of acute and chronic denervation associated with positive anti‐GM1 IgM antibodies. Conclusions: This case shows that nerve ultrasound can be a complementary tool for diagnosing multifocal motor neuropathy without typical electrodiagnostic features. Muscle Nerve 52: 294–299, 2015
Global cPILOT analysis of the APP/PS‐1 mouse liver proteome
Authors: Evans Adam R. , Gu Liqing , Guerrero Rodolfo , Robinson Renã A. S. 
Published: January 2015

PurposeA quantitative proteomics strategy called combined precursor isotopic labeling and isobaric tagging (cPILOT) was designed to discover alterations in the amyloid precursor protein/presenilin‐1 (APP/PS‐1) mouse liver proteome. The multiplexing strategy allows simultaneous quantitation of 12 samples in a single experiment.Experimental designFor cPILOT samples, six APP/PS‐1 and six heterozygous mouse livers were modified using precursor dimethylation (pH 2.5) followed by isobaric tagging (pH 8.0). Samples were pooled, fractioned with strong cation exchange, and analyzed using RPLC‐MS3 for protein identification and relative quantitation. In order to increase proteome coverage, a two‐tiered data collection strategy was employed. Six duplex precursor dimethylation experiments were also performed to verify cPILOT protein quantitation.ResultsThe combination of cPILOT with precursor dimethylation data resulted in 2437 total liver proteins identified and 77 differentially expressed proteins in APP/PS‐1 liver. Differentially expressed proteins are involved in metabolic processes such as B‐oxidation, pyruvate metabolism, and glucose regulation.Conclusions and clinical relevancecPILOT expands protein quantitation using isobaric tags and can be applied to any clinical laboratory interested in enhanced multiplexing strategies. Differentially expressed proteins in APP/PS‐1 mouse liver suggest the potential use of ketone bodies to alleviate metabolic dysregulation in Alzheimer's disease brain. Our work also suggests alterations in the alanine cycle potentially leading to hyperammonia production, may contribute to Alzheimer's disease pathogenesis.
Scientists' views about communication training
Authors: Besley John C. , Dudo Anthony , Storksdieck Martin 
Published: January 2015

AbstractThis study assesses how scientists think about science communication training based on the argument that such training represents an important tool in improving the quality of interactions between scientists and the public. It specifically focuses on training related to five goals, including views about training to make science messages understandable, as well as attitude‐focused training meant to build trust and credibility, to demonstrate that one listens to the public, to demonstrate that one cares about the public's views, and to frame messages to resonate with audiences' pre‐existing values. The theory of planned behavior and procedural justice theory were used to identify potential predictors of views about training toward these goals. Results show that the scientists rate message comprehension and credibility most favorably and give their lowest rating to training related to framing. Regression analyses reveal that believing that public engagement can make a difference (external efficacy) and belief in the ethicality of specific goals were the best predictors of whether scientists saw value in goal‐oriented training. The results suggest that communication trainers might benefit from emphasizing the effectiveness and ethicality of engagement activities if they want to attract scientists to communication training, and that more work may need to be done by professional organizations to help scientists consider the value of thinking about communication goals beyond the traditional focus on message comprehension. © 2015 Wiley Periodicals, Inc. J Res Sci Teach 52:199–220, 2015.