bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling cross-species feed intake responses to thermal stress
Authors: WHITE R. R. , HANIGAN M. D. 
Published: November 2015

SUMMARYThe objectives of the current study were to compare and model feed intake responses to ambient temperature across species and to assess opportunities to use cross-species (CS) data to parameterize models when species-specific (SS) data were limited. Literature searches were conducted to identify studies reporting intake during thermal stress compared with thermoneutral (TN) conditions. The resulting data set comprised 614 treatment means from 108 studies on livestock responses to thermal stress. An analysis of variance was conducted with the CS data set to identify the effects of species, temperature and species by temperature interactions on intake as (fractional feed intake; FFI). Four models were derived from the CS data set and root mean squared prediction error (RMSPE) and concordance correlation coefficients (CCC) of these models were compared with models of the same form derived from SS data sets. Models used explanatory variables for (1) duration of exposure; (2) mean temperature; (3) minimum and maximum temperatures; or (4) difference between minimum and maximum temperatures. An additional model accounting for temperature and stage of production was derived from the SS data. Analysis of variance demonstrated that the species by temperature interaction did not have a significant effect on FFI. Across species, intake decreased with temperature. Notably, all species demonstrated a constant decrease in intake across the TN zone indicating the previous assumption of constant intake during thermoneutrality may be not fully valid. When compared on a SS basis, SS-derived models had marginally lower RMSPE and higher CCC when compared with models derived from the CS data sets. The model fit with production data had the lowest RMSPE and highest CCC within the study. When compared over temperature ranges with minimal data available in some species (e.g., cold stress), using CS models often resulted in decreased RMSPE and improved CCC when compared with SS models. Although fitting models based on SS data allows for incorporating unique covariates, like level of production, fitting responses based on CS data can help to improve model estimates when knowledge gaps exist.
Electron flow through biological molecules: does hole hopping protect proteins from oxidative damage?
Authors: Winkler Jay R. , Gray Harry B. 
Published: November 2015

AbstractBiological electron transfers often occur between metal-containing cofactors that are separated by very large molecular distances. Employing photosensitizer-modified iron and copper proteins, we have shown that single-step electron tunneling can occur on nanosecond to microsecond timescales at distances between 15 and 20 Å. We also have shown that charge transport can occur over even longer distances by hole hopping (multistep tunneling) through intervening tyrosines and tryptophans. In this perspective, we advance the hypothesis that such hole hopping through Tyr/Trp chains could protect oxygenase, dioxygenase, and peroxidase enzymes from oxidative damage. In support of this view, by examining the structures of P450 (CYP102A) and 2OG-Fe (TauD) enzymes, we have identified candidate Tyr/Trp chains that could transfer holes from uncoupled high-potential intermediates to reductants in contact with protein surface sites.
Acceleration of reaction in charged microdroplets
Authors: Lee Jae Kyoo , Banerjee Shibdas , Nam Hong Gil , Zare Richard N. 
Published: November 2015

AbstractUsing high-resolution mass spectrometry, we have studied the synthesis of isoquinoline in a charged electrospray droplet and the complexation between cytochromecand maltose in a fused droplet to investigate the feasibility of droplets to drive reactions (both covalent and noncovalent interactions) at a faster rate than that observed in conventional bulk solution. In both the cases we found marked acceleration of reaction, by a factor of a million or more in the former and a factor of a thousand or more in the latter. We believe that carrying out reactions in microdroplets (about 1–15 μm in diameter corresponding to 0·5 pl – 2 nl) is a general method for increasing reaction rates. The mechanism is not presently established but droplet evaporation and droplet confinement of reagents appear to be two important factors among others. In the case of fused water droplets, evaporation has been shown to be almost negligible during the flight time from where droplet fusion occurs and the droplets enter the heated capillary inlet of the mass spectrometer. This suggests that (1) evaporation is not responsible for the acceleration process in aqueous droplet fusion and (2) the droplet–air interface may play a significant role in accelerating the reaction. We argue that this ‘microdroplet chemistry’ could be a remarkable alternative to accelerate slow and difficult reactions, and in conjunction with mass spectrometry, it may provide a new arena to study chemical and biochemical reactions in a confined environment.
A twisting story: how a single gene twists a snail? Mechanogenetics
Authors: Kuroda Reiko 
Published: November 2015

AbstractLeft–right (l–r) symmetry breaking and the establishment of asymmetric animal body plan during embryonic development are fundamental questions in nature. The molecular basis of l–r symmetry breaking of snails is a fascinating topic as it is determined by a maternal single handedness-determining locus at a very early developmental stage. This perspective describes the current state of the art of the chiromorphogenesis, mainly based on our own work, i.e. the first step of l–r symmetry breaking, as proven by our “Mechanogenetics”, before the start of zygotic gene expression, transfer of chirality information to the cell-fate determining stage, and the expression of nodal at the blastula stage. The Nodal signalling pathway is a common mechanism in vertebrates’ chiromorphogenesis in later development. Studies on snails, especiallyLymnaea(L.)stagnalis, shall give important insights into the molecular basis of chiromorphogenesis not only in Lophotrochozoa but in vertebrates as well.
Time-Dependent Resolution of Collagen Deposition During Skin Repair in Rats: A Correlative Morphological and Biochemical Study
Authors: Novaes Rômulo D. , Cupertino Marli C. , Sarandy Mariaurea M. , Souza André , Soares Evelise A. , Gonçalves Reggiani V. 
Published: November 2015

AbstractSkin samples were used to compare microscopy methods used to quantify collagen with potential applicability to resolve time-dependent collagen deposition during skin wound healing in rats. Skin wounds by secondary intention were made in rats and tissue fragments were collected every 7 days for 21 days. Collagen content determined by biochemical analysis was compared with collagen measured by point counting (PC) on histological skin sections stained by Gomori’s trichrome method (Trichrome/PC), Sirius red under polarized light (PL) microscopy (Sirius red/PL-PC), and computational color segmentation (CS) applied to sections stained with Sirius red (Sirius red/PL-CS). All microscopy methods investigated resolved the time-dependent dynamics of collagen deposition in scar tissue during skin wound healing in rats. Collagen content measured by Sirius red/PL-PC and Sirius red/PL-CS was significantly lower when compared with Trichrome/PC. The Trichrome/PC method provided overestimated values of collagen compared with biochemical analysis. In the early stages of wound healing, which shows high production of noncollagenous molecules, Sirius red/PL-CS and Sirius red/PL-PC methods were more suitable for quantification of collagen fibers. Trichrome staining did not allow clear separation between collagenous and noncollagenous elements in skin samples, introducing a marked bias in collagen quantification.
THE CONTRIBUTION OF L. G. KOVÁCS TO THE THEORY OF PERMUTATION GROUPS
Authors: PRAEGER CHERYL E. , SCHNEIDER CSABA 
Published: November 2015

The work of L. G. (Laci) Kovács (1936–2013) gave us a deeper understanding of permutation groups, especially in the O’Nan–Scott theory of primitive groups. We review his contribution to this field.
Mehr Stress durch G8?
Authors: Minkley Nina , Rest Miriam , Terstegen Susanna , Kirchner Wolfgang H. , Wolf Oliver T. 
Published: November 2015

Zusammenfassung. Ziel der vorliegenden Studie war es die Auswirkungen der Schulzeitverkürzung von der neunjährigen (G9) zur achtjährigen (G8) Gymnasialzeit auf die Stressbelastung von Schülern zu untersuchen. An der Studie nahmen insgesamt 69 Abiturienten des doppelten Abiturjahrganges 2013 in NRW teil. Von diesen wurde mit Hilfe der Cortisolkonzentration im Speichel und der subjektiven Stresseinschätzung die Stressbelastung im Schulalltag erfasst. Des Weiteren wurde auch die langfristige Stressbelastung vor und während der Abiturprüfungen betrachtet, indem die Cortisolkonzentration im Haar analysiert wurde. Hierbei zeigte sich, dass die Cortisolkonzentration der beiden untersuchten Schülergruppen im Tagesverlauf sehr ähnlich ist, wobei die G8-Schülerinnen in der Tendenz eine leicht höhere Cortisolkonzentration aufweisen. Auf das subjektive Stressempfinden hat die Schulbiographie jedoch keinen Einfluss. Des Weiteren steigt die Cortisolkonzentration im Haar beider Schülergruppen zwischen der Vorprüfungs- und Prüfungsphase signifikant an, was darauf hindeutet, dass die Prüfungsphase einen langfristigen Stressor darstellt.
Unusual Deep Water sponge assemblage in South China—Witness of the end-Ordovician mass extinction
Authors: Li Lixia , Feng Hongzhen , Janussen Dorte , Reitner Joachim 
Published: November 2015

AbstractThere are few sponges known from the end-Ordovician to early-Silurian strata all over the world and no records of sponge fossils have been found yet in China during this interval. Here we report a unique sponge assemblage spanning the interval of the end-Ordovician mass extinction from the Kaochiapien Formation (Upper Ordovician-Lower Silurian) in South China. This assemblage contains a variety of well-preserved siliceous sponges, including both Burgess Shale-type and modern type taxa. It is clear that this assemblage developed in deep water, low energy ecosystem with less competitors and more vacant niches. Its explosion may be related to the euxinic and anoxic condition as well as the noticeable transgression during the end-Ordovician mass extinction. The excellent preservation of this assemblage is probably due to the rapid burial by mud turbidites. This unusual sponge assemblage provides a link between the Burgess Shale-type deep water sponges and the modern forms. It gives an excellent insight into the deep sea palaeoecology and the macroevolution of Phanerozoic sponges and opens a new window to investigate the marine ecosystem before and after the end-Ordovician mass extinction. It also offers potential to search for exceptional fossil biota across the Ordovician-Silurian boundary interval in China.
The C-terminal Domain Supports a Novel Function for CETPI as a New Plasma Lipopolysaccharide-Binding Protein
Authors: García-González Victor , Gutiérrez-Quintanar Nadia , Mas-Oliva Jaime 
Published: November 2015

AbstractDescribed by our group a few years ago, the cholesteryl-ester transfer protein isoform (CETPI), exclusively expressed in the small intestine and present in human plasma, lacked a functional identification for a role of physiological relevance. Now, this study introduces CETPI as a new protein with the potential capability to recognise, bind and neutralise lipopolysaccharides (LPS). Peptides derived from the C-terminal domain of CETPI showed that CETPI not only might interact with several LPS serotypes but also might displace LPS bound to the surface of cells. Peptide VSAK, derived from the last 18 residues of CETPI, protected against the cytotoxic effect of LPS on macrophages. At high concentrations, when different cell types were tested in culture, it did not exhibit cytotoxicity by itself and it did prevent the expression of pro-inflammatory cytokines as well as the generation of oxidative stress conditions. In a rabbit model of septic shock, the infusion of peptide VSAK exerted a protective effect against the effects of LPS and reduced the presence of tumor necrosis factor-alpha (TNFα) in plasma. Therefore, CETPI is proposed as a new protein with the capability to advance the possibilities for better understanding and treatment of the dangerous effects of LPS in vivo.
Impacts of El Niño Southern Oscillation and Indian Ocean Dipole on dengue incidence in Bangladesh
Authors: Banu Shahera , Guo Yuming , Hu Wenbiao , Dale Pat , Mackenzie John S. , Mengersen Kerrie , Tong Shilu 
Published: November 2015

AbstractDengue dynamics are driven by complex interactions between hosts, vectors and viruses that are influenced by environmental and climatic factors. Several studies examined the role of El Niño Southern Oscillation (ENSO) in dengue incidence. However, the role of Indian Ocean Dipole (IOD), a coupled ocean atmosphere phenomenon in the Indian Ocean, which controls the summer monsoon rainfall in the Indian region, remains unexplored. Here, we examined the effects of ENSO and IOD on dengue incidence in Bangladesh. According to the wavelet coherence analysis, there was a very weak association between ENSO, IOD and dengue incidence, but a highly significant coherence between dengue incidence and local climate variables (temperature and rainfall). However, a distributed lag nonlinear model (DLNM) revealed that the association between dengue incidence and ENSO or IOD were comparatively stronger after adjustment for local climate variables, seasonality and trend. The estimated effects were nonlinear for both ENSO and IOD with higher relative risks at higher ENSO and IOD. The weak association between ENSO, IOD and dengue incidence might be driven by the stronger effects of local climate variables such as temperature and rainfall. Further research is required to disentangle these effects.
Lactobacillus rhamnosus GG Lysate Increases Re-Epithelialization of Keratinocyte Scratch Assays by Promoting Migration
Authors: Mohammedsaeed Walaa , Cruickshank Sheena , McBain Andrew J. , O’Neill Catherine A. 
Published: November 2015

AbstractA limited number of studies have investigated the potential of probiotics to promote wound healing in the digestive tract. The aim of the current investigation was to determine whether probiotic bacteria or their extracts could be beneficial in cutaneous wound healing. A keratinocyte monolayer scratch assay was used to assess re-epithelialization; which comprises keratinocyte proliferation and migration. Primary human keratinocyte monolayers were scratched then exposed to lysates of Lactobacillus (L) rhamnosus GG, L. reuteri, L. plantarum or L. fermentum. Re-epithelialization of treated monolayers was compared to that of untreated controls. Lysates of L. rhamnosus GG and L. reuteri significantly increased the rate of re-epithelialization, with L. rhamnosus GG being the most efficacious. L. reuteri increased keratinocyte proliferation while L. rhamnosus GG lysate significantly increased proliferation and migration. Microarray analysis of L. rhamnosus GG treated scratches showed increased expression of multiple genes including the chemokine CXCL2 and its receptor CXCR2. These are involved in normal wound healing where they stimulate keratinocyte proliferation and/or migration. Increased protein expression of both CXCL2 and CXCR2 were confirmed by ELISA and immunoblotting. These data demonstrate that L. rhamnosus GG lysate accelerates re-epithelialization of keratinocyte scratch assays, potentially via chemokine receptor pairs that induce keratinocyte migration.
Age-associated changes in rich-club organisation in autistic and neurotypical human brains
Authors: Watanabe Takamitsu , Rees Geraint 
Published: November 2015

AbstractMacroscopic structural networks in the human brain have a rich-club architecture comprising both highly inter-connected central regions and sparsely connected peripheral regions. Recent studies show that disruption of this functionally efficient organisation is associated with several psychiatric disorders. However, despite increasing attention to this network property, whether age-associated changes in rich-club organisation occur during human adolescence remains unclear. Here, analysing a publicly shared diffusion tensor imaging dataset, we found that, during adolescence, brains of typically developing (TD) individuals showed increases in rich-club organisation and inferred network functionality, whereas individuals with autism spectrum disorders (ASD) did not. These differences between TD and ASD groups were statistically significant for both structural and functional properties. Moreover, this typical age-related changes in rich-club organisation were characterised by progressive involvement of the right anterior insula. In contrast, in ASD individuals, did not show typical increases in grey matter volume and this relative anatomical immaturity was correlated with the severity of ASD social symptoms. These results provide evidence that rich-club architecture is one of the bases of functionally efficient brain networks underpinning complex cognitive functions in adult human brains. Furthermore, our findings suggest that immature rich-club organisation might be associated with some neurodevelopmental disorders.
A striking reduction of simple loudness adaptation in autism
Authors: Lawson Rebecca P. , Aylward Jessica , White Sarah , Rees Geraint 
Published: November 2015

AbstractReports of sensory disturbance, such as loudness sensitivity or sound intolerance, are ubiquitous in Autism Spectrum Disorder (ASD) but a mechanistic explanation for these perceptual differences is lacking. Here we tested adaptation to loudness, a process that regulates incoming sensory input, in adults with ASD and matched controls. Simple loudness adaptation (SLA) is a fundamental adaptive process that reduces the subjective loudness of quiet steady-state sounds in the environment over time, whereas induced loudness adaptation (ILA) is a means of generating a reduction in the perceived volume of louder sounds. ASD participants showed a striking reduction in magnitude and rate of SLA relative to age and ability-matched typical adults, but in contrast ILA remained intact. Furthermore, rate of SLA predicted sensory sensitivity coping strategies in the ASD group. These results provide the first evidence that compromised neural mechanisms governing fundamental adaptive processes might account for sound sensitivity in ASD.
Habitual Sleep Duration and Risk of Childhood Obesity: Systematic Review and Dose-response Meta-analysis of Prospective Cohort Studies
Authors: Ruan Huijuan , Xun Pengcheng , Cai Wei , He Ka , Tang Qingya 
Published: November 2015

AbstractA meta-analysis of cross-sectional studies found that shorter-time sleep was correlated with increased risk of obesity in children. However, findings from prospective cohort studies were inconsistent. PubMed and other data resources were searched through May 2015. Twenty-five eligible studies were identified including 56,584 children and adolescents with an average 3.4-year follow-up. Compared with children having the longest sleep duration (~12.2 hours), kids with the shortest sleep duration (~10.0 hours) were 76% more likely to be overweight/obese (pooled odds ratio [OR]: 1.76; 95% confidence interval [CI]: 1.39, 2.23); and had relatively larger annual BMI gain (pooled β coefficient: 0.13; 95% CI: 0.01, 0.25 kg/m2). With every 1 hour/day increment in sleep duration, the risk of overweight/obesity was reduced by 21% (OR: 0.79; 95% CI: 0.70, 0.89); and the annual BMI gain declined by 0.05 kg/m2 (β = −0.05; 95% CI: −0.09, −0.01). The observed associations were not appreciably modified by region, baseline age or the length of follow-up. Accumulated literature indicates a modest inverse association between sleep duration and the risk of childhood overweight/obesity. Further research is needed to determine the age and gender specified optimal hours of sleep and ideal sleep pattern with respect to obesity prevention in children.
Green synthesis of in situ electrodeposited rGO/MnO2 nanocomposite for high energy density supercapacitors
Authors: Rusi  , Majid S. R. 
Published: November 2015

AbstractThis paper presents the preparation ofin situelectrodeposited rGO/MnO2nanocomposite as a binder-free electrode for supercapacitor application. The work describes and evaluates the performance of prepared electrode via green and facile electrodeposition technique ofin siturGO/MnO2-glucose carbon nanocomposites. The carbon content in the composite electrode increased after GO and D (+) glucose solution has been added in the deposition electrolyte. This study found that a suitable concentration of D (+) glucose in the deposition electrolyte can slow down the nucleation process of MnO2particles and lead to uniform and ultrathin nanoflakes structure. The optimize electrode exhibited low transfer resistance and resulted on excellent electrochemical performance in three electrolyte systems viz. Na2SO4, KOH and KOH/K3Fe(CN)6redox electrolytes. The optimum energy density and power density were 1851 Whkg−1and 68 kWkg−1at current density of 20 Ag−1in mixed KOH/K3Fe(CN)6electrolyte.
An integrative quantifier of multistability in complex systems based on ecological resilience
Authors: Mitra Chiranjit , Kurths Jürgen , Donner Reik V. 
Published: November 2015

AbstractThe abundance of multistable dynamical systems calls for an appropriate quantification of the respective stability of the (stable) states of such systems. Motivated by the concept of ecological resilience, we propose a novel and pragmatic measure called ‘integral stability’ which integrates different aspects commonly addressed separately by existing local and global stability concepts. We demonstrate the potential of integral stability by using exemplary multistable dynamical systems such as the damped driven pendulum, a model of Amazonian rainforest as a known climate tipping element and the Daisyworld model. A crucial feature of integral stability lies in its potential of arresting a gradual loss of the stability of a system when approaching a tipping point, thus providing a potential early-warning signal sufficiently prior to a qualitative change of the system’s dynamics.
Eryptosis Indices as a Novel Predictive Parameter for Biocompatibility of Fe3O4 Magnetic Nanoparticles on Erythrocytes
Authors: Ran Qian , Xiang Yang , Liu Yao , Xiang Lixin , Li Fengjie , Deng Xiaojun , Xiao Yanni , Chen Li , Chen Lili , Li Zhongjun 
Published: November 2015

AbstractFe3O4magnetic nanoparticles (Fe3O4-MNPs) have been widely used in clinical diagnosis. Hemocompatibility of the nanoparticles is usually evaluated by hemolysis. However, hemolysis assessment does not measure the dysfunctional erythrocytes with pathological changes on the unbroken cellular membrane. The aim of this study is to evaluate the use of suicidal death of erythrocytes (i.e. eryptosis indices) as a novel predictive and prognostic parameter and to determine the impact of Fe3O4-MNPs on cellular membrane structure and the rheology properties of blood in circulation. Our results showed that phosphatidylserine externalization assessment was significantly more sensitive than classical hemolysis testing in evaluating hemocompatibility. Although no remarkable changes of histopathology, hematology and serum biochemistry indices were observedin vivo, Fe3O4-MNPs significantly affected hemorheology indices including erythrocyte deformation index, erythrocyte rigidity index, red blood cell aggregation index and erythrocyte electrophoresis time, which are related to the mechanical properties of the erythrocytes. Oxidative stress induced calcium influx played a critical role in the eryptotic activity of Fe3O4-MNPs. This study demonstrated that Fe3O4-MNPs cause eryptosis and changes in flow properties of blood, suggesting that phosphatidylserine externalization can serve as a predictive parameter for hemocompatibility assay.