bluuebunny/crossref_metadata_2025_split huggingface dataset:
The landscape of clinical trials in corneal regeneration: A systematic review of tissue engineering approaches in corneal disease
Authors: Boroumand Safieh , Rahmani Mahya , Sigaroodi Faraz , Ganjoury Camellia , Parandakh Azim , Bonakdar Alireza , Khani Mohammad‐Mehdi , Soleimani Masoud 
Published: July 2024

AbstractThe limited availability of a healthy donor cornea and the incidence of allograft failure led researchers to seek other corneal substitutes via tissue engineering. Exploring the trend of clinical trials of the cornea with the vision of tissue engineering provides an opportunity to reveal future potential corneal substitutes. The results of this clinical trial are beneficial for future study designs to overcome the limitations of current therapeutic approaches. In this study, registered clinical trials of bio‐based approaches were reviewed for corneal regeneration on March 22, 2024. Among the 3955 registered trials for the cornea, 392 trials were included in this study, which categorized in three main bio‐based scaffolds, stem cells, and bioactive macromolecules. In addition to the acellular cornea and human amniotic membrane, several bio‐based materials have been introduced as corneal substrates such as collagen, fibrin, and agarose. However, some synthetic materials have been introduced in recent studies to improve the desired properties of bio‐based scaffolds for corneal substitutes. Nevertheless, new insights into corneal regenerative medicine have recently emerged from cell sheets with autologous and allogeneic cell sources. In addition, the future perspective of corneal regeneration is described through a literature review of recent experimental models.
Treatment comparison of hydroxyurea versus ruxolitinib in essential thrombocythaemia: A matched‐cohort analysis
Authors: Grunwald Michael R. , Ritchie Ellen K. , Rumi Elisa , Assad Albert , Hamer‐Maansson J. E. , Yu Jingbo , Kalafut Tricia , Braunstein Evan , Passamonti Francesco 
Published: July 2024

AbstractHydroxyurea is the preferred first‐line cytoreductive treatment for high‐risk essential thrombocythaemia (ET), but many patients are intolerant or refractory to hydroxyurea. Ruxolitinib has been shown to improve symptoms in patients with ET. This post hoc analysis compared the clinical outcomes of patients with ET who received hydroxyurea only with those who switched from hydroxyurea to ruxolitinib due to intolerance/resistance to hydroxyurea. Patients with ET refractory/intolerant to hydroxyurea treated with ruxolitinib in a completed phase 2 study (HU‐RUX) were propensity score matched with patients who received hydroxyurea only in an observational study (HU). Changes in leukocyte and platelet counts were reported at 6‐month intervals during the 48‐month follow‐up. Following propensity score matching, 37 patients were included for analysis in each cohort. Mean (standard deviation [SD]) leukocyte and platelet counts at index were higher for HU‐RUX versus HU (leukocyte: 9.3 [5.1] vs. 6.8 [3.1] × 109/L; platelet: 1027.4 [497.8] vs. 513.9 [154.7] × 109/L), both of which decreased significantly from index to 6 months through to 48 months in HU‐RUX (mean [SD] change from index at 6 months—leukocyte: −1.8 [4.6] × 109/L; platelet: −391.7 [472.9] × 109/L; at 48 months—leukocyte: −3.8 [5.3] × 109/L; platelet: −539.0 [521.8] × 109/L), but remained relatively stable in HU (mean [SD] change from index at 6 months—leukocyte: 0 [1.8] × 109/L; platelet: −5.7 [175.3] × 109/L; at 48 months—leukocyte: −0.1 [2.7] × 109/L; platelet: −6.9 [105.1] × 109/L). In conclusion, these results demonstrate that switching from hydroxyurea to ruxolitinib in patients with ET who are intolerant or refractory to hydroxyurea could improve abnormal haematologic values similar to those who receive first‐line hydroxyurea.
Reliability and changes in knee cartilage T2 relaxation time from 6 to 24 months after anatomic anterior cruciate ligament reconstruction
Authors: Nukuto Koji , Gale Tom , Yamamoto Tetsuya , Kamada Kohei , Irrgang James J. , Musahl Volker , Anderst William 
Published: July 2024

AbstractThe objectives of this study were to evaluate the reliability of cartilage T2 relaxation time measurements and to identify focal changes in T2 relaxation on the affected knee from 6 to 24 months after anatomic anterior cruciate ligament reconstruction (ACLR). Data from 41 patients who received anatomic ACLR were analyzed. A bilateral 3.0‐T MRI was acquired 6 and 24 months after ACLR. T2 relaxation time was measured in subregions of the femoral condyle and the tibial plateau. The root‐mean‐square coefficient of variation (RMSCV) was calculated to evaluate the reliability of T2 relaxation time in the contralateral knee. Subregion changes in the affected knee T2 relaxation time were identified using the contralateral knee as a reference. The superficial and full thickness layers of the central and inner regions showed good reliability. Conversely, the outer regions on the femoral side and regions in the deep layers showed poor reliability. T2 relaxation time increased in only 3 regions on the affected knee when controlling for changes in the contralateral knee, while changes in T2 relaxation time were identified in 14 regions when not using the contralateral knee as a reference. In conclusion, evaluation of cartilage degeneration by T2 relaxation time after ACLR is most reliable for central and inner cartilage regions. Cartilage degeneration occurs in the central and outer regions of the lateral femoral condyle from 6 to 24 months after anatomic ACLR.
Perioperative management protocol for pediatric endoluminal functional lumen imaging probe in esophageal motility disorders
Authors: Mansi Sherief , Dorfman Lev , El‐Chammas Khalil , Santucci Neha , Graham Khaleb , Fei Lin , Wittkugel Eric , Levi Stacy , Kaul Ajay 
Published: July 2024

AbstractObjectivesLower esophageal sphincter achalasia is associated with a higher risk of aspiration during anesthesia. Endoluminal Functional Lumen Imaging Probe (EndoFLIP) is used as an adjunctive tool in both the diagnosis and treatment of achalasia, for which all children require anesthesia. Anesthesia may affect the parameters of the EndoFLIP due to its effect on gut motility. There are no standard anesthesia protocols to help decrease the risk of aspiration and the undesirable effect of anesthesia on EndoFLIP parameters. This study aims to standardize an anesthesia protocol to target both goals.MethodsA protocol was developed to address perioperative management in patients undergoing EndoFLIP for any indication to minimize both anesthetic effect on the esophageal motility as well as perioperative complications. A retrospective data analysis was conducted on patients who underwent EndoFLIP at Cincinnati Children's Hospital Medical Center; pre‐ and post‐protocol implementation data including adverse events was compared.ResultsPre‐protocol implementation: 60 cases (median age of 13.8 years, 30 [50%] females) with 2 cases of adverse events (3.3%). Post‐protocol implementation: 71 cases (median age of 14.6 years, 37 [52.1%] females) with no adverse events (0/71 = 0%). In comparison between pre‐ and post‐protocol cases, no significant difference was noted in gender, age, and adverse events. Post‐protocol procedures were found to be significantly shorter (median time of 89 vs. 79 min, p = 0.004).ConclusionsOur anesthesia protocol provides a standardized way of administering anesthesia minimizing impact on EndoFLIP parameters and aspiration for patients with achalasia.
What drives tourists to take a digital‐free tourism?
Authors: Liu Jia‐Qi , Jeon Chang‐Young , Yang Hee‐Won 
Published: July 2024

AbstractThis study utilizes a combined model of two social cognitive models, protection motivation theory and theory of planned behavior, to analyze how smartphone use triggers the demand for DFT. For this purpose, this study conducted an online survey and used the quota sampling method. Of the collected response sheets, a total of 534 valid samples were used in the final analysis. The analysis verified the hypothesis using confirmatory factor analysis, correlation analysis, and structural equation modeling. This study presented that regarding threat and coping appraisals of smartphone use, perceived vulnerability and response efficacy positively affect protection motivation. And perceived subjective norm affects protection motivation. This result of study fills a gap in the field of DFT research through quantitative research and provides practical evidence that helps to capture the needs of DFT tourists and provides suggestions for the construction of DFT environments.
Organic matter accumulation drives methylotrophic methanogenesis and microbial ecology in a hypersaline coastal lagoon
Authors: Keneally Christopher , Southgate Matilda , Chilton Daniel , Gaget Virginie , Welsh David T. , Mosley Luke , Erler Dirk V. , Kidd Stephen P. , Brookes Justin 
Published: July 2024

AbstractHypersalinity is common in coastal wetlands throughout warm, tropical, and arid regions. Climate‐induced changes in rainfall, sea level, and anthropogenic modification to basins and coastlines are likely to further increase salinization in these ecosystems. Yet, carbon cycling in hypersaline coastal wetlands is not well understood, and poorly constrained in climate models. In the Coorong, a eutrophic, hypersaline coastal lagoon, recognized as internationally important under the Ramsar convention, organic matter rapidly accumulates in deeper areas of the lagoon, through the settling of fine detrital particles, phytoplankton and suspended sediments. During initial surveys, elevated surface water methane (CH4) concentrations were observed above these fine depositional sediments. To identify the drivers of CH4 production, organic matter and sediment characteristics were assessed in surface sediments. Genetic markers (i.e., 16rDNA and the mcrA functional gene) were used to characterize microbial communities. With multiple lines of evidence, this study identifies organic matter, methanogen abundance, and salinity as important drivers of CH4 production, which is concentrated in depositional zones. Archaea were also more abundant in depositional zones, including methylotrophic methanogens: Methanofastidiosales, Methanomasiliicoccales, Methermicoccaceae, and Methanococcoides. These methanogens were highly correlated to CH4 in porewater, suggesting an influence of methylotrophic methanogenesis. To investigate further, metabolic genes were predicted from 16S rRNA with PICRUSt2. This represents the first effort to analyze CH4 dynamics in the Coorong, underscoring the need to integrate these unique ecosystems into global climate models to enhance our understanding of greenhouse gas dynamics and emissions in a changing climate.
Poly(Butylene Succinate) Copolyesters Modified with Linear‐Chain Diols toward Adjustable Thermal, Mechanical, and Biodegradable Properties
Authors: Wang Qiang , Yu Jiahao , Yu Chunhui , Zhang Chunlei , Wei Chao , Xiao Yan 
Published: July 2024

AbstractThe production and application of poly(butylene succinate) (PBS) still face challenges such as high production costs, insufficient toughness, and slow biodegradation. This study utilizes the ring‐opening condensation polymerization method to prepare PBS copolyesters using succinic anhydride (SAA) and 1,4‐butanediol (BDO) as raw materials, with 20 moL% different lengths of linear‐chain diols as third monomers (carbon numbers are 3, 5, 6, 8, 9,10, and 12). Both PBS and its copolyesters exhibit high weight average molecular weights (18.8 × 104–26.1 × 104 g moL−1), much higher than those obtained through the traditional conventional direct esterification method. Incorporating the third monomer reduces the glass transition temperature (Tg), crystallinity, and melting point (Tm) of the copolyesters. As the chain length of the third monomer increases, the copolyesters show improved toughness, with the elongation at break and notch impact strength of poly(butylene succinate‐ran‐dodecylene succinate) (P(BS‐ran‐DoS)) increasing from 374.1% and 5.6 KJ m−2 of PBS to 723.2% and 64.8 KJ m−2, respectively. The degradation rate of the copolyesters modified with short‐chain diols increases significantly, and as the chain length of the third monomer increases, the degradation rate of the copolyesters slows down. Therefore, the selection of the third monomer can be used to adjust the properties of the polymer.
Navigating intellectual property challenges in China: Insights from RCEP and domestic factors
Authors: Wu Zhixiao , Qin Qian 
Published: July 2024

AbstractIn a high‐quality economic era, China needs innovative development. Intellectual property (IP) innovation and development are the first driving forces in China. At the same time, China has signed the Regional Comprehensive Economic Partnership agreement (RCEP), which has had a significant impact on the development of China's IP law. Under the combination of these two factors, China's IP innovation and development environment are worth studying. This paper uses the “factor analysis method” from five dimensions, including initiatives for civic awareness, regional IP development foundation, legislative support, law enforcement measures, and judicial protection, to study the development environment of IP innovation in 31 provincial regions in China, in the past 3 years. It is found that IP development in various regions of China is insufficient in some way, and the scores of different provinces vary greatly, so it will inevitably affect the development of China's IP integrally and then affect the construction process of strong provinces and cities with intellectual property rights (IPR). The novelty of this research is its in‐depth investigation into how the RCEP and global partnerships are influencing the transformation of China's intellectual property landscape. This transformation requires legal adjustments and underscores the importance of specialized IP judge training, alignment with international norms, and regional IP progress to realize China's ambition of becoming a dominant force in intellectual property. Combined with the fact that RCEP has come into effect in China and has had a significant impact, it is necessary to develop China's IP undertaking from legislative, judicial, law enforcement, and international cooperation aspects.
Cordyceps sinensis ameliorates idiopathic pulmonary fibrosis in mice via inhibiting mitochondrion‐mediated oxidative stress
Authors: Zhang Ying , Zhou Lirun , Cheng Guangqing , Zhou Yanyan , Guo Qiuyan , Wu Jiangpeng , Wong Yin K. , Zhang Junzhe , Tang Huan , Wang Jigang 
Published: July 2024

AbstractIdiopathic pulmonary fibrosis (IPF) represents a chronic interstitial lung disease with an unclear underlying mechanism and currently lacks a definitive treatment. Cordyceps sinensis (CS), renowned for its pharmacological properties in traditional Chinese medicine and extensive use in lung disease treatment, holds promise as a therapeutic agent for IPF. However, the specific role of CS in treating IPF remains unclear. In this study, we aimed to assess the efficacy of CS in treating IPF and unravel potential underlying mechanisms. Our results demonstrate that CS treatment effectively mitigated pulmonary inflammation and collagen deposition in bleomycin‐induced IPF mice. Proteomics analysis revealed that the regulation of mitochondrial oxidative phosphorylation may serve as a potential protective mechanism of CS against IPF in mice. Further investigation unveiled that CS could suppress the excessive production of mitochondrial reactive oxygen species in lung tissues induced by bleomycin through moderating the expression and activity of mitochondrial complexes, thus safeguarding the integrity and function of mitochondria. Overall, our findings not only underscore the effectiveness of CS in preventing bleomycin‐induced IPF but also highlight mitochondrial‐mediated oxidative stress as a promising therapeutic target for treating IPF.
HPClas: A data‐driven approach for identifying halophilic proteins based on catBoost
Authors: Hu Shantong , Wang Xiaoyu , Wang Zhikang , Jiang Menghan , Wang Shihui , Wang Wenya , Song Jiangning , Zhang Guimin 
Published: July 2024

AbstractHalophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean‐up, and energy production. Generally, halophilic proteins are discovered and characterized through labor‐intensive and time‐consuming wet lab experiments. In this study, we introduce the Halophilic Protein Classifier (HPClas), a machine learning‐based classifier developed using the catBoost ensemble learning technique to identify halophilic proteins. Extensive in silico calculations were conducted on a large public dataset of 12,574 samples and HPClas achieved an area under the receiver operating characteristic curve (AUROC) of 0.844 on an independent test set of 200 samples. The source code and curated dataset of HPClas are publicly available at https://github.com/Showmake2/HPClas. In conclusion, HPClas can be explored as a promising tool to aid in the identification of halophilic proteins and accelerate their application in different fields.
Strict glucose control and elimination of NLRP3‐induced inflammation prevents diabetic bladder dysfunction in the female Akita mouse model
Authors: Hughes Francis M. , Harper Shelby N. , Jin Huixia , Odom Michael R. , Purves J. Todd 
Published: July 2024

AbstractPurposeDiabetic bladder dysfunction (DBD) is the most common diabetic complication. Logically, regulation of blood glucose should reverse dysfunction, but the Epidemiology of Diabetes Interventions and Complications study found strict control ineffective. However, it is possible that strict control may prevent DBD if initiated before symptoms appear. We examine the effect of early glucose control on development of DBD in the female diabetic Akita mouse (Type 1) and test the potential of inhibiting/deleting NLRP3 as adjunct therapy to glucose control.Materials and MethodsFemale Akita mice were bred NLRP3+/+ or NLRP3−/−. At 6 weeks of age, diabetics received either no glucose control or insulin pellets (s.c., Linshin) designed to poorly or strictly control blood glucose. At Week 15, blood glucose (glucometer), the extravasation potential of bladder (an indirect measurement of inflammation) and bladder function (urodynamics) were assessed.ResultsBlood glucose of diabetics was reduced in poorly controlled and strongly reduced in strictly controlled groups. Levels were not affected by deletion of NLRP3. Evans blue dye extravasation correlated with glucose control and was eliminated in the NLRP3−/− groups. Urodynamics found markers of overactivity in diabetics which was improved in the poorly controlled group and eliminated in the strictly controlled group. In the NLRP3−/− mice, no bladder dysfunction developed, regardless of glucose control.ConclusionsEarly‐initiated strict glycemic control and NLRP3 elimination can effectively prevent DBD, suggesting hyperglycemia acts through NLRP3‐induced inflammation to trigger DBD.
Switching from intermittent catheterization with single‐use catheter to a reusable catheter has a negative impact on quality of life
Authors: Schnipper Julie , Azawi Nessn , Størling Zenia , Simonsen Kenneth Starup , Andersen Karin 
Published: July 2024

AbstractPurposeIt has been proposed that reusable catheters are more cost effective and environmentally sustainable than single‐use catheters intended for intermittent catheterization (IC). However, the aspect of individuals' well‐being and preference for catheter type is not considered. In this study, we investigated the impact on individuals' health‐related quality of life (HR‐QoL) when testing a reusable catheter.Materials and MethodsThe study was an open‐labeled, single‐arm, multicenter investigation with a treatment period of 28 days. Forty subjects using single‐use hydrophilic catheters were accustomed to a reusable catheter for managing IC. HR‐QoL was evaluated by the Intermittent‐Self Catheterization Questionnaire (ISC‐Q). Additionally, satisfaction was evaluated by the Intermittent Catheterization Satisfaction Questionnaire (InCaSa‐Q). The difference in total score was analyzed using a mixed linear model. Furthermore, preference for IC (single‐use vs. reusable) was assessed and microbial evaluation of the catheters was performed.ResultsThe total ISC‐Q score measuring HR‐QoL decreased significantly by 28% (p < 0.001). Two of the four subdomains (ease‐of‐use and discreetness) also decreased significantly (p < 0.001). The total InCaSa‐score and all four subdomains evaluating satisfaction decreased significantly (p < 0.005). The primary study results were supported by the fact that 90.9% of subjects preferred to use a single‐use catheter for IC. Furthermore, 50% of reusable catheters were contaminated with bacteria.ConclusionSwitching from single‐use to reusable IC resulted in a significant decrease in HR‐QoL and satisfaction. Moreover, the vast majority preferred the single‐use catheter due to handling and convenience. The users' rights to their preferred bladder management method should be acknowledged.
Exploring career choices of specialist nurse students: Their decision‐making motives. A qualitative study
Authors: Tiliander Annika , Olsson Caroline , Kalèn Susanne , Ponzer Sari , Fagerdahl Ami 
Published: July 2024

AbstractAimsTo explore Registered Nurses' motives to undergo specialist training and to choose a particular speciality.DesignA descriptive qualitative interview study.MethodsSemi‐structured interviews were conducted during 2021 with 20 Swedish specialist nurse students from different specialisation areas. Qualitative content analysis was used. The COREQ checklist was used to report the study.ResultsSpecialist nurse students' motivations for further training were divided into three main categories with two sub‐categories each. The main categories were ‘toward new challenges and conditions in work life’, ‘contributions to the development and higher competencies in health care’ and ‘personal work and life experiences as ground for choice’.ConclusionOur study demonstrates the importance of motivating factors in the career choices of Specialist nurse students, such as personal challenges, desirable working conditions, career growth opportunities and personal experiences in the career choices. Creating a supportive work environment that helps to prioritise work‐life balance and offers the development of new skills might help retain nurses.No Patient or Public ContributionNo patient or public contribution was used. However, if more nurses would choose to undergo specialist training, especially in areas facing significant shortages, it would most likely lead to improved health‐related outcomes for patients or populations.