bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversible Inhibition of Keratinocyte Thymidine Incorporation by the Calmodulin Antagonist, W‐7
Authors: Hirokawa Masaki , Hashimoto Yoshio , Ohkuma Noritaka , Mizumoto Toshihiro , Iizuka Hajime 
Published: April 2015

AbstractAlthough calmodulin has been suggested as an important regulator of keratinocyte proliferation, its precise role remains unknown. We employed a calmodulin antagonist, N‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalenesulfonamide (W‐7), to examine the role of calmodulin on keratinocyte proliferation. N‐(6‐aminohexyl)‐1‐naphthalenesulfonamide (W‐5), a chlorine‐deficient analogue of W‐7 with little anti‐calmodulin activity, was used as the control. W‐7 markedly inhibited thymidine incorporation of pig epidermis at concentrations close to its anti‐calmodulin activity; W‐5 had no effect on the thymidine incorporation. The inhibitory effect of W‐7 was reversible; the removal of W‐7 from the incubation medium resulted in the reinitiation of the thymidine incorporation, suggesting that W‐7 is not a cytotoxic agent. These results are consistent with the view that calmodulin is an essential regulator of keratinocyte proliferation. The epidermal beta‐adrenergic response, which is decreased in various hyperproliferative epidermal abnormalities, was increased in W‐7‐treated hypoproliferative epidermis. The epidermal SOD activity, which is also decreased in the hyperproliferative epidermis, however, was not affected by the W‐7 treatment.
Effects of Human Recombinant Tumor Necrosis Factor‐α (TNF‐α) on the Proliferative Potential of Human Keratinocytes Cultured in Serum‐free Medium
Authors: Kono Takeshi , Tanii Tsukasa , Furukawa Masayoshi , Mizuno Nobuyuki , Taniguchi Shoji , Ishii Masamitsu , Hamada Toshio 
Published: April 2015

AbstractThe effects of human recombinant tumor necrosis factor‐α (TNF‐α) on human keratinocytes cultured in a serum‐free medium were investigated. TNF‐α markedly suppressed cell growth. The growth‐inhibitory effect was reversible and cytostatic at a concentration of 1–5 U/ml, but appeared to be irreversible and cytocidal at 10 U/ml. The growth suppressive effect was more marked when TNF‐α was added in the late growth phase or preconfluent phase than when it was added in early or mid‐growth phases. No effects of TNF‐α on cell adhesion to the substrate were observed. These results indicate that TNF‐α is a very potent anti‐proliferative agent for human keratinocytes.
Kaposi's Sarcoma: A Light and Electron Microscopic Study
Authors: Kato Haruhisa , Hamada Toshio , Tsuji Takuo , Baba Takashi , Seki Jun‐ichi , Kobayashi Yasutsugu 
Published: April 2015

AbstractA patient with diabetes mellitus who developed the typical classic lesions of Kaposi's sarcoma is described. Our patient presented with a reddish‐purple papulonodular lesion on the right foot of five months' duration. A skin biopsy specimen showed a proliferation of spindle cells forming numerous vascular slits and a diffuse extravasation of erythrocytes. The patient's sera was negative for human immunodeficiency virus (HIV) antibodies and cytomegalovirus (CMV) antibodies. Ultrastructural examination demonstrated fibroblast‐like spindle cells phagocytosing and digesting red blood cells to form vascular spaces. The patient died, due to gastrointestinal hemorrhage, and the autopsy revealed an extensive visceral involvement of Kaposi's sarcoma.
Significance of Elevated Serum LDH (Lactate Dehydrogenase) Activity in Atopic Dermatitis
Authors: Mukai Hideki , Noguchi Toshihiko , Kamimura Kimio , Nishioka Kiyoshi , Nishiyama Shigeo 
Published: April 2015

AbstractSerum lactate dehydrogenase activity (LDH) was elevated in most cases with the severe type of atopic dermatitis (AD). We examined whether LDH correlated specifically with the clinical courses and the severity of AD skin eruptions. Blood eosinophil numbers (Eo), LDH and its isoenzymes, and serum IgE (IgE) levels in eighty patients with AD were measured before and after treatment.In improved groups, Eo and LDH decreased or returned to normal values after treatment. In contrast, both levels increased in patients who worsened after treatment. Thus changes in Eo and LDH correlated well with the clinical picture. However, IgE levels did not correlate with the clinical condition. High LDH levels were detected in the severe type of AD more frequently than in the mild type. A large majority of patients with elevated LDH (higher than 300 IU/1) before treatment had more severe skin eruptions. When the LDH before treatment was within normal limits, the LDH levels in improved patients showed a further decrease. Eo, LDH and IgE were correlated closely with the clinical scores of AD, and the relationship between LDH and clinical score was the highest. Elevations of LDH5 and LDH4 among the LDH isoenzymes were more prominent in the severe type of AD than in the mild type.We concluded that LDH was useful as a marker for evaluating the disease condition and severity of skin eruption of AD.
Infundibular Keratosis
Authors: Keong Chin‐Huai , Maruyama Ryuji , Katsumata Michio 
Published: April 2015

AbstractTwo cases of infundibular keratosis are presented, characterized histopathologically by funnel‐shaped, invaginating, epithelial lobules showing infundibular keratinization and connection with pilosebaceous structures. Lightly‐staining, glycogen‐laden epithelial cells predominated within the two tumors. Differential diagnoses with other infundibular tumors and relationships to trichilemmoma are discussed.
Action of Staphylococcal Exfoliative Toxins on Epidermal Cell Cultures and Organotypic Skin
Authors: Gentilhomme Edqar , Faure Michel , Piemont Yves , Binder Pierre , Thivolet Jean 
Published: April 2015

AbstractIn the staphylococcal scalded skin syndrome, spontaneous intraepithelial cleavages are due to the exfoliative toxins A or B (ETA or ETB). Until now, these toxins have been studied either on epidermis or on organotypic skin cultures. In the present study, we compare the effects of these toxins on human keratinocyte cell cultures to those on human and mouse organotypic skin cultures. With concentrations of ETA or ETB of 1 mg/ml for 3 hours, spontaneous intraepithelial cleavages were noted in both cell and organotypic cultures. Keratinocyte cell cultures were as sensitive as organotypic skin cultures to these toxins. Since keratohyaline granules may represent a possible binding site for ETA or ETB, we tried to correlate the expression of keratohyaline granules with the appearance of intraepithelial clefts due to the toxins. However, when cultured in liquid medium, epithelia were not differentiated enough to allow the detection of the binding site of ETA‐ETB.
The Local Recurrence of Pigmented Spitz Nevus after Removal
Authors: Tanaka Keiko , Mihara Motoyuki , Shimao Shuhei , Taniguchi Kaoru 
Published: April 2015

AbstractA seventeen‐month‐old female had a pigmented nodule on her left lower leg. The excised lesion was histologically diagnosed as a Spitz nevus, composed mainly of spindle‐shaped melanocytes containing large amounts of melanin pigment. When nodular regrowth was seen at the operative site, the recurrent lesion was radically excised as nodular melanoma. However the histological characteristics of the second excised specimen were essentially the same as those in the initially excised one except for the existence of the newly formed collagen fibers, which may suggest an involuting stage in the central portion of the lesion.
Photoprotective Effect of Topically Applied Superoxide Dismutase on Sunburn Reaction in Comparison with Sunscreen
Authors: Hamanaka Hiroshi , Miyachi Yoshiki , Imamura Sadao 
Published: April 2015

AbstractPhotoprotective effect of topically applied superoxide dismutase (SOD) to guinea pig skin was compared with a commercially available sunscreen agent after a single exposure to UVB. While cutaneous SOD activity was remarkably decreased in non‐treated control animals, both topical SOD and sunscreen agent significantly reduced the decrease in skin SOD activity after UVB irradiation. However, only the sunscreen agent successfully reduced erythema reaction 24h after irradiation but topical SOD failed. These findings suggest that topical SOD protects skin from photo‐oxidative damage without affecting erythema response, and thus, from a practical standpoint, sunscreen agents, when compared with topical antioxidants, seem better at present for daily photoprotection.
Unilateral Dermatomal Superficial Telangiectasia
Authors: Okinaga Miyako , Go Kikyo , Mizoguchi Masako 
Published: April 2015

AbstractA three‐year‐old boy with unilateral dermatomal superficial telangiectasia (UDST) is the first reported case with skin lesions in two non‐adjacent areas (dermatomal areas of Trigeminal2 and L4–5). In this case, onset occurred at an early age, the onset was eight months after an operation, and the liver function was normal; therefore we believe it is congenital, even though the patient had undergone an extensive right hepatectomy to remove a hamartoma in his liver at the age of four months.
Hemangiopericytoma: Characteristic Features Observed by Magnetic Resonance Imaging and Angiography
Authors: Kato Naoko , Kato Sadatoshi , Ueno Hiroo 
Published: April 2015

AbstractHemangiopericytoma is a rare vascular tumor which may originate wherever there are capillaries. We report a case of this tumor occurring in a 79‐year‐old Japanese man, which originated from the subcutaneous tissues of the left upper arm and reached a size of 6 × 5 × 4 cm. Histopathological and electron microscopical findings were compatible with those of benign‐type hemangiopericytoma. Magnetic resonance imaging showed the most intense signal on proton density images. Angiography showed the characteristic tumor feeder artery forming a pedicle which entered the tumor and branched radially and a dense, uniform, well‐demarcated, oval tumor stain. These images were helpful in performing proper operative treatment without noteworthy bleeding. These modern modalities are excellent non‐invasive diagnostic procedures for the vascular tumors of soft tissues in dermatology.
Epidermal Langerhans' Cells in Elderly Patients with Decubital Ulcers
Authors: Kohn Sarah , Kohn David , Schiller Dan 
Published: April 2015

AbstractLangerhans' cells in the sacral epidermis 8–10 cm from the lesion of elderly patients (mean age 74 years) with decubital ulcers were studied ultrastructurally and compared with the patients' own normal epidermis from the upper leg, with age‐matched normal controls, and with young normal controls (mean age 43 years). High percentages of Langerhans' cells (ranging between 30 and 50%) without dendrites were found in patient epidermis from the sacral region near the lesion and similar percentages in normal skin from the patients' upper leg. In both elderly and young controls, Langerhans' cells without dendrites found in the epidermis of the upper leg were fewer, ranging between 13 and 33%. The density of Langerhans' cells in adjacent sites of the same epidermis was non‐homogeneous, being in the range of 0.56–1.19% in patient sacral epidermis, 0.69–1.31% in patient normal leg epidermis, 0.63–4.17% in leg epidermis of the elderly controls, and 0.63–3.94% in leg epidermis of the young controls. The majority of the Langerhans' cells in both patients and controls were located near the basal cell layer and in the mid‐epidermis. The percentage of Langerhans' cells found in patient sacral epidermis (0.84 ± 0.08%) and in their leg epidermis (0.87 ± 0.15%) was significantly lower than in the elderly controls (1.91 ± 0.30%) and young controls (1.84 ± 0.24%). The low percentage of Langerhans' cells in the epidermis of patients with decubital ulcers may affect the healing process of the lesions.
Wells' Syndrome (Eosinophilic Cellulitis)
Authors: Wong Kit‐Ching , Hwang Jen‐Kan , Wong Chu‐Kwan 
Published: April 2015

AbstractWe report a case of Wells' syndrome (eosinophilic cellulitis) with acute cutaneous swelling followed by indolent infiltration. The histopathology is characterized by a dense infiltrate of eosinophils and “flame figures” in the dermis. The electron microscopic findings are peculiar. This case responded well to oral antihistamines. The skin signs and symptoms disappeared completely two weeks later.
Traumatic Neuroma: Multiple Lesions in the Fingers Occurring after Deep Burns
Authors: Ono Tomomichi , Matsunaga Wakatoshi 
Published: April 2015

AbstractA 6‐year‐old Japanese girl developed five asymptomatic nodules at the amputated edges of her right index, middle, and ring fingers. The amputation had been performed after she suffered deep burns at the age of 3 months. A histological diagnosis of traumatic neuroma was made.
Effect of 1,24R‐dihydroxyvitamin D3 on the Growth of Human Keratinocytes
Authors: Matsumoto Kunio , Hashimoto Koji , Kiyoki Mamoru , Yamamoto Masaru , Yoshikawa Kunihiko 
Published: April 2015

AbstractThe effect of 1,24R‐dihydroxyvitamin D3 (1,24R(OH)2D3), a synthetic analogue of a biologically active form of vitamin D3 (1,25‐dihydroxyvitamin D3, 1,25(OH)2D3), on the growth of human keratinocytes cultured in serum‐free medium was investigated. The growth of cultured normal human keratinocytes was inhibited by 65% by 10−8M 1,24R(OH)2D3 and by 90% by 10−7M 1,24(OH)2D3. It inhibited cell growth almost completely at 10−6M. The DNA synthesis of keratinocytes was also inhibited with 1,24R(OH)2D3 by 27% at 10−8M, 59% at 10−7M, and 92% at 10−6M. The inhibition of cell growth and DNA synthesis were more remarkable by 1,24R(OH)2D3 than by 1,25(OH)2D3. 1,24R(OH)2D3 also inhibited the growth of keratinocytes derived from patients with psoriasis vulgaris; the growth inhibitory effect was again more remarkable with 1,24R(OH)2D3 than with 1,25(OH)2D3. The viability and protein synthesis of keratinocytes were not affected by 1,24R(OH)2D3, suggesting that the growth inhibitory effect is due to its biological activity, not to cytotoxicity. The binding of [3H]‐labeled 1,25(OH)2D3 to its receptor in the cytosolic fraction of cultured keratinocytes was competitively substituted by unlabeled 1,24R(OH)2D3 as well as 1,25(OH)2D3, suggesting that 1,24R(OH)2D3 binds to the 1,25(OH)2D3 receptor. It was found that the affinity of 1,24R(OH)2D3 for the receptor was slightly higher than that of 1,25(OH)2D3. These results demonstrate that 1,24R(OH)2D3 functions as a potent growth inhibitor in vitro in human keratinocytes from both normal and psoriatic epidermis, and it possesses a higher affinity for the 1,25(OH)2D3 receptor in cultured human keratinocytes. The difference in affinity of 1,24R(OH)2D3 for the 1,25(OH)2D3 receptor correlates with its greater inhibition of keratinocyte growth than 1,25(OH)2D3. 1,24R(OH)2D3 may be useful in the treatment of psoriasis.
Cutaneous Reactions Caused by Experimental Exposure to Jellyfish, Carybdea rastonii
Authors: Ohtaki Noriko , Oka Keiko , Sugimoto Akiko , Akizawa Toshihiko , Yasuhara Tadashi , Azuma Hiroshi 
Published: April 2015

AbstractDermatitis caused by contact with tentacles of jellyfish was studied on 25 volunteers. Two tentacles cut from a living jellyfish, Carybdea rastonii, were applied on each of the forearms and skin reactions were observed.All volunteers complained of severe pain, which lasted from 10 min to 8 hrs. Erythema and wheal appeared within 3 to 4 min and enlarged for 15 to 20 min. Erythema subsided within 24 hrs to 3 days in all but two individuals.Seven to 13 days after the application, linear erythema and papulo‐vesicular lesions with pruritus were observed on the forearms of 15 out of 25 volunteers tested. These flare‐up lesions lasted for one week leaving slight pigmentation.Histological findings from the flare‐up lesions corresponded to those of allergic contact dermatitis.The lymphocyte response to the jellyfish venom in the subjects who had recurring lesions was greater than that in either the subjects with no recurring lesions or the control group, who was never exposed to jellyfish.
Disseminated Coccidioidomycosis
Authors: Jitsukawa Kumiko , Sato Syozo , Hayashi Yoko , Yamao Hiroyuki , Anzai Takashi 
Published: April 2015

AbstractWe report a case of disseminated coccidioidomycosis in a 39‐year‐old Japanese male whose illness developed after returning from a trip to an endemic area. He showed positive coccidioidin skin reaction throughout the entire course of his illness. The primary lesion in the lung subsequently spread to the bone. While the patient was on treatment with 5‐FC, he made another trip to the same endemic area. After this episode, he developed pulmonary symptoms and cutaneous nodules on his wrist. The possibility of reinfection with Coccidioides immitis is discussed. Electron microscopy of the cutaneous nodules revealed that the spherules examined maintained their structural integrity in the granulomatous lesion, suggesting the high viability of the organism. Host‐parasitic interaction in coccidioidomycosis is discussed.
Pseudoelephantiasis of the Penis Following Donovanosis
Authors: Sehgal Virendra N. , Sharma Hemant K. 
Published: April 2015

AbstractPseudoelephantiasis of the penis, an unusual sequel of longstanding donovanosis with otherwise characteristics clinical expression, is reported in a young man. The diagnosis was confirmed by the demonstration of Donovan bodies in Giemsa stained tissue smears and tissue sections.
HLA in Systemic Scleroderma (PSS) and Familial Scleroderma
Authors: Sasaki Tetsuo , Denpo Kenichi , Ono Hideki , Nakajima Hiroshi 
Published: April 2015

AbstractHLA in systemic scleroderma (PSS), including three familial cases, is reported. Three families in which one sister developed PSS and another sister suffered from either PSS (family 1), mixed connective tissue disease (MCTD) (family 2), or Sjögren's syndrome (SjS) (family 3) were described. The elder sister in family 1 died of respiratory insufficiency caused by scleroderma lung. The sisters in family 2 both had SjS, anti SS‐A antibodies, and HLA A2‐Bw55‐Cwl‐DRw8 haplotype in common. The elder sister with PSS in family 3 also had SjS and Hashimoto's thyroiditis. HLA in 28 PSS patients including these 3 familial cases were analyzed with 4 MCTD and 4 generalized morphea patients. HLA A2, Bw46, DR2, DRw8, DRw6 and DQw1 antigens were more frequently found in the PSS patients than in the controls. HLA DRw6 was the only antigen that was positive in common in the 3 familial cases. In those patients with anti topoisomerase I antibodies, HLA DR2 antigen was found more frequently than in the controls. Some, but not all, of these results were similar to the previous reports on HLA in PSS. Further investigations on more patients and the other members of these families would be necessary to clarify the significance of these results.
Clinical and Histological Analysis of Labial Lip Biopsy in Sjögren Syndrome
Authors: Katayama Ichiro , Nishiyama Shigeo , Nishioka Kiyoshi 
Published: April 2015

AbstractLabial lip biopsy findings from 52 patients with Sjögren syndrome (SjS) and cutaneous manifestations were clinically analyzed.Findings of the labial lip biopsy with more than one focus (a cluster of at least 50 lymphocytes) were positive in 52% (28/52). Sixteen patients who presented with oral dryness had negative labial lip biopsy findings with less than one focus score and five patients without oral dryness had positive results. Cells infiltrating into the salivary gland were categorized as plasma cell rich (Group 1), lymphocyte rich (Group 3), intermediate (Group 2), or no gland in biopsied specimens (Group 4). Sicca complaint and the grade of lip biopsy score were significantly higher in Group 3 than in Group 1; no differences were observed in laboratory findings between Groups 1 and 3. The patterns of age distribution and salivary flow rate were also not statistically significant in these two groups. The patients with plasma cell rich infiltrate presented oral dryness, although their clinical and grade of lip biopsy scores were relatively low; most of these patients have secondary SjS. Some of the patients in Group 4 showed an advanced stage of the disease though their lip biopsy score was grade 1 with atrophic or no gland. These data suggest that labial lip biopsy is a useful screening method for the diagnosis of Sjögren syndrome and that clinical manifestations may differ in parallel with the type of infiltrating cell.
Superoxide Dismutase Activity of Murine Ultraviolet Radiation‐induced Fibrosarcoma Cell Strains
Authors: Hamanaka Hiroshi , Miyachi Yoshiki , Moriwaki Shin‐ichi , Nishigori Chikako , Imamura Sadao 
Published: April 2015

AbstractThe average superoxide dismutase (SOD) activity of seven cell strains from mouse skin fibrosarcoma induced by ultraviolet (UV) irradiation was 5.78 ± 0.81 (mean ± SE) unit/mg protein, while that of seven normal mouse skin fibroblasts was 5.36 ± 1.58. Although tumor cells have been previously reported to exhibit reduced SOD activity, the present study demonstrates that there is comparable SOD activity between tumor cells and normal cells of the same tissue origin.
Tinea of the Glans Penis: Report of a Case Presenting as a Crop of Papules
Authors: Dekio Satoshi , Qin Li‐mo , Jidoi Joji 
Published: April 2015

AbstractA 27‐year‐old Japanese male student with tinea of the glans penis was described. The lesion on the glans penis was unique; it developed as a crop of papules.
Non‐invasive Measurement of the Vascular Dynamics of Dermographism
Authors: Hornstein Otto Paul , Boissevain Friederike , Wittmann Harald 
Published: April 2015

AbstractThere is no dissent about the vascular origin of dermographism (D); however, the microcirculatory events underlying this phenomenon are not yet elucidated sufficiently. In particular, the vascular mechanisms producing the white D pattern in atopics are a matter of divergent hypotheses. In order to quantify the D phenomenon reproducibly, we constructed an easily usable device called “Dermographometer” which enables us to apply constant stretching pressure to the skin. We were able to investigate several parameters of microvascular cutaneous reaction to defined skin stretching pressure by laser‐Doppler‐microfluxmetry and infrared‐thermography. These measurements were performed on 23 patients with atopic eczema (AE) and 21 healthy controls under standardized investigative conditions. Only patients under similar therapeutic regimens (no corticosteroids) with dry or lichenified skin inflammation (lumbar area) were included in the study.The basic values of laser‐Doppler‐fluxmetry (LDF) showed a significant reduction in the intensity of hyperemia in the patients from those in the normal controls; this reaction depended on the visual degree of the dermographic blanching effect (white, delayed white, indifferent, pale‐red). Patients with white or indifferent D had the lowest rises in blood flux; those with delayed white or pale‐red D had more elevated blood fluxes, but these were still clearly below the mean levels of normal red D. Infrared‐thermography showed a significant diminution of both the rise and plateau phase of the radiating temperature in comparison to the controls.Our results support the hypothesis that white D (including different subtypes of its pallor) depends on the degree of local vasoconstriction, possibly in combination with altered blood flow in cutaneous shunt vessels.
Late Onset Dermal Melanocytosis: An Upper Back Variant
Authors: Ono Tomomichi , Egawa Kiyofumi , Kayashima Ken‐ichi , Kitoh Masato 
Published: April 2015

AbstractSeven elderly Japanese men with blue macules on their upper backs are reported. Histological examination showed this pigmentation to be melanosis due to the presence of spindle‐shaped or oval pigment‐laden cells in the dermis. Electron microscopy revealed the cells to be compatible with dermal melanocytes. We assume that this condition belongs to the category of late onset dermal melanocytosis and is an upper back variant. This concept has not been proposed before.