bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of CFTR intronic variants identified during cystic fibrosis population screening by a minigene splicing assay
Authors: Giorgi Gianpietro , Casarin Alberto , Trevisson Eva , Donà Marta , Cassina Matteo , Graziano Claudio , Picci Luigi , Clementi Maurizio , Salviati Leonardo 
Published: October 2015

AbstractCystic fibrosis, caused by mutations of theWe have examined fiveFour out of five variants (including c.2620-26A>G which was previously reported as a possible splice-site mutation) did not alter the correct splicing of the minigene and are likely to be neutral polymorphisms, whereas c.744-6T>G caused complete skipping ofHybrid minigenes assay are a simple and rapid tool to evaluate the effects of intronic variants without the need of analyzing patient’s mRNA, and are particularly suited to analyze variants identified during population screenings.
Additive Archaeology: An Alternative
Framework for Recontextualising
Archaeological Entities
Authors: Reilly Paul 
Published: October 2015

Abstract
Additive manufacturing poses a number of challenges to conventional understandings of
materiality, including the so-called archaeological record. In particular, concepts such as real, virtual,
and authentic are becoming increasingly unstable, as archaeological artefacts and assemblages can be
digitalised, reiterated, extended and distributed through time and space as 3D printable entities. This paper
argues that additive manufacturing represents a ‘grand disciplinary challenge’ to archaeological practice
by offering a radical new generative framework within which to recontextualise and reconsider the nature
of archaeological entities specifically within the domain of digital archaeology.
Deferred Consent for Randomized Controlled Trials in Emergency Care Settings
Authors: Harron Katie , Woolfall Kerry , Dwan Kerry , Gamble Carrol , Mok Quen , Ramnarayan Padmanabhan , Gilbert Ruth 
Published: October 2015

BACKGROUND:
There is limited experience in using deferred consent for studies involving children, which was legalized in the United Kingdom in 2008. We aimed to inform future studies by evaluating consent rates and reasons for nonconsent in a large randomized controlled trial in pediatric intensive care.

METHODS:
In the CATCH trial, eligible children from 14 PICUs in England and Wales were randomly assigned to 3 types of central venous catheters. To avoid delay in treatment, children admitted on an emergency basis were first randomly assigned to a trial central venous catheter, and we deferred seeking consent to use already collected data and blood samples until after stabilization.

RESULTS:
Consent was obtained for 984/1358 (72%) of children admitted on an emergency basis. Failure to obtain consent resulted mainly from a lack of opportunity (early discharge or transfer) for survivors and difficulties in seeking consent for children who died. For admissions where there was an opportunity to approach (n = 1298), inclusion rates differed according to survival status: 93/984 (9%) of consented patients died, compared with 58/314 (18%) of nonconsented patients. For children admitted on an emergency basis whose families were approached, 984/1178 (84%) provided deferred consent (n = 15 sites), compared with 441/641 (69%) of children admitted on an elective basis who were approached for prospective consent (n = 9 sites).

CONCLUSIONS:
Design of emergency randomized controlled trials should balance the potential burden that seeking consent in difficult situations may cause against risk of bias by disproportionately excluding children who die or are transferred. Ethics committees could consider approving the use of already collected data when best efforts to obtain deferred consent are unsuccessful.
CHEMOTHERAPY OF TUBERCULOSIS: PROBLEMS AND PERSPECTIVES
Authors: Vasil’eva I. A. , Samoilova A. G. , Ergeshov A. E. , Bagdasaryan T. R. , Chernousova L. N. 
Published: October 2015

Challenges of tuberculosis chemotherapy under conditions of high drug resistant (DR) tuberculosis burden are discussed. Ultimate results of treatment by standard regimens of 1658 patients with new tuberculosis cases and relapses were analyzed. Favorable ultimate results were observed among both patients with new tuberculosis cases and relapses having drugs sensitivity. Efficacy of tuberculosis treatment by standard regimens of chemotherapy is decreasing as a result of DR amplification. Risk factors of unfavorable ultimate result among pulmonary tuberculosis patients are primary resistance to isoniazid (OR =2,1) and multiple drug resistance of M. tuberculosis (OR =8,0). Earlier onset of treatment and correct individual therapy with second line drugs as a result of rapid methods of DR tuberculosis diagnostics are those approaches which provide the best therapeutic effect among multiple drug resistant tuberculosis patients both in culture conversion (97,7%) and cavity closure rate (82,7%).
CLINICAL AND EXPERIMENTAL RESEARCH OF IMMUNOMODULATORY EFFECT OF AMISULPRIDE
Authors: Vetlugina T. P. , Lobacheva O. A. , Al'perina E. L. , Zhukova E. N. , Semke A. V. , Nikitina V. B. , Cheido M. A. , Idova G. V. 
Published: October 2015

Study of immunomodulatory effect of atypical antipsychotic amisulpride has revealed a positive clinical effect after 6-week therapy of schizophrenic patients regarding both positive and negative symptoms. A decrease in activity of humoral immunity factors (В lymphocytes, immunoglobulins, HLA-DR+-cells) identified among schizophrenic patients in the process of amisulpride therapy can be attributed to a positive effect optimizing the ratio Th1/Th2. Amisulpride when used under experimental conditions produced a suppression of IgM-immune response in mice of the C57BL/6J strain. This effect was more expressed in animals with aggressive behavior pattern.
AGONIST OF V2 VASOPRESSIN RECEPTOR REDUCES DEPRESSIVE DISORDERS IN POST-STROKE PATIENTS
Authors: Belokoskova S. G. , Stepanov I. I. , Tsikunov S. G. 
Published: October 2015

Poststroke depression is one of the common psychiatric complications after stroke. Thus, the research of new ways for treatment depressed mood after stroke is actual. The previous researches revealed vasopressin to be effective in patients with memory, speech and motor function disorders after stroke. The purpose of the study was to investigate influence of vasopressin on depression after stroke. Fourteen patients with affective disorders have been treated with subendocrine doses of 1-desamino-8-D-arginin-vasopressin (DDAVP) daily by intranasal application during 1,5–2 months. Vasopressin was effective in correcting both apatoadinamic and anxious depression. Treatment effect was durable, lasts for 0,5–1 year after the first course of therapy. The results of this pilot study demonstrate perspective of using selective agonist of vasopressin V2 receptors, DDAVP, in therapy of post-stroke depression.
THE FUNDAMENTS OF NEURONAL PLASTICITY
Authors: Skrebitskii V. G. , Shtark M. B. 
Published: October 2015

Plasticity of the nervous system is determined by the modification of efficacy of synaptic transmission: long- term potentiation and long- term depression. Different modern technical approaches such as: registration of ionic currents in single neuron, molecular- genetic analysis, neurovisualization, and others reveal the molecular mechanisms of synaptic plasticity. The understanding of these mechanisms, in its turn, stimulates the development of methods of pharmacological correction of different forms of brain pathology such as Alzheimer disease, parkinsonism, alcoholism, aging and others.
Plant Simulation as an Instrument of Logistics and Transport of Materials in a Digital Factory
Authors:   Technical University of Kosice Faculty of Mechanical Engineering, Department of Safety and Quality Production, Slovakia, Hovanec Michal , Píľa Ján , Korba Peter , Pačaiová Hana 
Published: October 2015

The competition of international manufacturing networks creates pressure to increase the efficiency of manufacturing systems. In addition to this, indicative for the present period is the fact that the number of technical components in many products is increasing, and at the same time the requirements are growing for corresponding assembly, transport and logistical processes. These requirements can be administered and recognized in the scope of their complexity only through the use of an array of rapid and efficient methods in the form of a digital factory. This contribution points to the practical application of the Plant Simulation software module from the portfolio of the digital factory of Siemens PLM. This complex instrument with an environment for controlling the life cycle of a product enables repeated utilization of data, supports effective cooperation between individual units, and provides relevant data for each user who needs it, mainly from the viewpoint of strategic decision-making. The simulation of the entire flow of material, including all relevant divisions of manufacturing, storing and transport activities, is considered as the key component of the digital factory in industry and from this day forward is the most often used.
Adequacy of Estimation Model of Asymptotic Learning Operator – Pilot Function
Authors:   Technical University of Košice, Faculty of Aeronautics, Slovakia, Kurdel Pavol , Adamčík František , Labun Ján 
Published: October 2015

Aviation ergatic systems models are used in the design of concrete aviation systems and their adequacy is evaluated in the process of their design. Generally, a concrete ergatic process is realised with the help of simulation technology. Computer systems of recent and perspective flight simulators contain the architectures of hybrid information systems. Due to this, analogue as well as discrete methods of estimation of ergatic systems parametric sensitivity do not lose their practical importance.
A Proposal for the Optimization of Storage Areas in a Selected Enterprise
Authors:   Department of Transport and Logistics,  Institute of Technology and Business in České Budějovice, Czech Republic, Němec František , Lorincová Silvia , Potkány Marek , Raušer Daniel 
Published: October 2015

The current tough competition prevalent within the market economy is forcing business entities to achieve efficiency in their processes. This also applies to the transfer of goods from point A to point B, which is the subject of transportation. This process is influenced by many factors. It is necessary to not only consider the time,i.e. the fact that the required goods must be delivered in time, but it is also essential to take the financial aspect linked into account, for example, with the cost items such as fuel, salaries etc. These and many other factors also influence the movement of goods within a warehouse, in which the business entity must maximize the space required for the storage of the goods, while minimizing the time necessary for the storage, and equally while optimizing the cost. The aim of this paper follows from the above, which is based on the facts of the course of storage operations in the chosen enterprise,to design an optimal layout of the storage area which should increase the efficiency of the logistical operations.
The Different Concentrations of Transforming Growth Factor-Beta1 (TGF-Beta1), Matrix Metalloproteinase-9 (MMP-9) and Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) in Normoalbuminuria Normotension, Normoalbuminuria Hypertension
Authors: Wijaya Johnson , Donosepoetro Marsetio , Bakri Syakib 
Published: October 2015

BACKGROUND: Vascular remodeling was an adaptive process of the vascular wall that occured in response to long-term changes in hemodynamic conditions that contribute to the changes of the vascular structure and the pathophysiology of vascular disease. On the other hand, Endothelial Progenitor Cells (EPC) derived from bone marrow had the capacity to migrate to the peripheral circulation and to differentiate into mature endothelial cells. Therefore, EPC could contribute in the endothelial repairing after endothelial injury.METHODS: This study was a cross sectional design. Analysis was done among 30 subjects with normoalbuminuria normotension, 55 subjects with normoalbuminuria hypertension and 30 subjects with with microalbuminuria hypertension. TGF-β1, MMP-9 and VEGFR-2 testing were performed by ELISA method. All statistical calculations were performed using the SPSS 11.5 statistical software package. We used the Independent sample T test, Mann-Whitney, One Way Annova and Kruskal Wallis to establish the difference among various biochemical measures.RESULTS: TGF-β1 concentration was increased from normoalbuminuria normotension to normoalbuminuria hypertension and to microalbuminuria hypertension (27.63178±12.97246 vs. 38.61193±17.09546 vs. 38.73939±12.63911 ng/mL). TGF-β1 concentration was higher significantly in normotension as compared to hypertension (27.63178±12.97246 vs. 38.65692±15.58950, p<0.001) and to microalbuminuria hypertension (38.73939±12.63911, p<0.001). MMP-9 concentration was increased in normotension to normoalbuminuria hypertension but was decreased in microalbuminuria hypertension (438.1967±156.4268 vs. 564.5873±291.2876 vs. 418.6900±188.3801 ng/mL). MMP-9 concentration was higher significantly in normoalbuminuria hypertension as compared to microalbuminuria hypertension (p=0.028). VEGFR-2 concentration was decreased from normotension to normoalbuminuria hypertension to microalbuminuria hypertension (9.90552±1.85185 vs. 9.39561±1.75413 vs. 9.00506±1.47173 ng/mL). VEGFR-2 concentration was higher significantly in normotension as compared to microalbuminuria hypertension (p=0.042). CONCLUSIONS: The increasing concentration level of TGF-β1 and decreasing concentration level of MMP-9 in microalbuminuria hypertension showed that the remodeling process was getting increased. The decreasing concentration level of VEGFR-2 in microalbuminuria hypertension showed that the repairing process was getting decreased.KEYWORDS: vascular remodelling, vascular repairing, TGF-β1, MMP-9, VEGFR-2
Porphyromonas gingivalis Induced Fragmentation of Type IV Collagen Through Macrophage-Activated MMP-9: (In Vitro Study of Collagenolytic Mechanism in Pathogenesis of Atherosclerotic Plaque Rupture)
Authors: Mubarokah Siti Nurul , Susilawati I Dewa Agung , Sumarno Sumarno , Muliartha I Ketut Gedhe , Sargowo Djanggan 
Published: October 2015

BACKGROUND: Periodontitis is caused mostly by Porphyromonas gingivalis (P.gingivalis) and it is related to acute coronary syndrome. P.gingivalis readily invades blood circulation and potentially induces collagenolytic activity of inflammatory cells that results in collagen vascular degradation leading to atherosclerotic plague rupture (APR). APR is responsible for the occurence of fatal cardiovascular events such as acute myocardial infraction (AMI).AIMS: To show that P.gingivalis potentially induces fragmentation of the type IV vascular collagen due to macrophage-activated MMP-9.MATERIAL AND METHODS: The ability of P.gingivalis to induce the type IV collagen fragmentation, shown by digesting type IV collagen with the supernatant of monocyte-derived macrophage activated by exposure to P.gingivalis suspension for 18 hours, 37oC, 5%CO2. The type IV collagen fragments were analyzed by SDS-PAGE and confirmed by Western-blotting. Antibody of type IV collagen produced and confirmed by dot-blotting prior to its being used as primary antibody of Western-blotting. The existence of MMP-9 was detected by Dot-blot and Western-blot technique, while the MMP-9 activity was assessed by SDS-PAGE and zymograms.RESULTS: Our data showed that P.gingivalis induced macrophage to produce MMP-9 as one of collagenolytic components, and interaction with P.gingivalis proteases enhanced the proteolytic activity and resulted in degradation of type IV collagen with molecular weight of 88 kDa into two smaller fragments with molecular weight of 80 kDa and 60 kDa. CONCLUSION: P.gingivalis induced macrophage to activate its MMP-9 that led to fragmentation of vascular type IV collagen in the pathogenesis of atherosclerotic plaque rupture. KEYWORDS: P.gingivalis, macrophage, type IV collagen fragmentation, atherosclerotic plaque rupture, AMI
Failure to apply for ethical approval for health studies in low-income countries
Authors: Van Teijlingen Edwin , Simkhada Padam 
Published: October 2015

On too many occasions researchers conduct public health and/or epidemiological studies in low-income countries without the appropriate in-country ethical approval. This article reflects on some of the underlying reasons for not applying for ethical approval. The piece concludes that we need to start by educating our (junior) researchers and research students about the importance of research ethics. We conclude with a number of recommendations for researchers, scientific journal editors and reviewers and ethical committees in high-income countries to bring the message home to researchers that ethical approval should be sought in low-income countries if and when required!
Relations entre les arpenteurs et leurs employeurs au XVIIIe siècle dans la région lilloise
Authors: Desreumaux Roger 
Published: October 2015

J.-B. Carpentier, a Lille estate agent, trusted with the management of land owned by the nobility in Flanders, Hainault, Brabant and Picardy, provides a good example of the relations between surveyors and their employers. According to the principle that : « no estate without a thorough survey is ever known in full detail », he resorts to the help of many land- surveyors, having them work on many of his current affairs.
In Sainghin-en-Mélantois, a stratagem enables Carpentier to get hold of the survey drawn by his competitor, which he has rapidly copied by one of his own surveyors. Another document, dated 1572, will help him to have the plan of the Abbey estate drawn anew.
The Slype « Commanderie » will bring Carpentier many a joy, thanks to surveyor Ramault. This later will represent him at the estimation of land claimed by the public authority for the opening of a new road. The estate agent hopes he will be able to do without a survey for the smaller La Braque « Commanderie », near Turnhout, but he will finally have to have one drawn, though a a cheaper cost.
Thus Carpentier uses the surveyors for the copy of ancient plans, the drawing of new ones, field inquiries, land estimations. He also uses them he takes part in the « lords'reac- tion » of his time, a component of the French Revolution.
« Voluptas et commoditas ». Architecture et vie sociale au XVIIIe siècle : l'exemple lillois
Authors: Duthoy Jean-Jacques 
Published: October 2015

Als organisator van feestelijkheden, ontwerper van vluchtige decors, maar ook als verantwoordelijke voor de aanleg van wandelingen, parken en plaatsen voor de ontspan- ning van het publiek, is de architect van het einde van de xvnie eeuw niet alleen in dienst van een privé-cliënteel : hij heeft ook een sociale functie. De architectuur is als kunst van de gemeenschap eng daarmee verbonden en schept de ruimte voor het dagelijkse leven en de ontspanning. In Rijsel zijn er de feesten georganiseerd door MichelLequeux en de bouw van het beroemde Colisée, een werk van François Verly.
Het is een uiting van een opvatting van wat wij nu de ruimtlijke omgeving (« environment ») noemen, een doordacht en vaak gelukkig mengsel van stad en platteland,steen en plant. Tijdens de laatste jaren van het Ancien Régime biedt Rijsel zijn inwoners sierlijk- heid en gemak. Het was zijn mooiste tijd.
Le retour de captivité du général Vandamme
Authors: Milot Jean 
Published: October 2015

Vandamme's papers and correspondence, kept in the Lille municipal library provide a new insight into this man's so discredited and disputed life in his time.
Among other things, they show how, coming back from his capitivity in Russia (July 1814), this general, one of the most consistently charged and denounced of the Revolution and Empire, always presented, even nowadays, as a money-grasping shark, and a covetous and interested calculator, was the victim of his good faith and ingenuousness.
Having decided, in order to hasten his return from Riga to Dunkirk, to sail back, he hired out a Danish vessel, the Coff Anna Dorothea, whose little scrupulous captain and owners tried to make him bear expenses out of proportion with the service supplied. Followed a trial full of unexpected reboundings, interrupted and delayed by the political situation and the General's exile to the United States of America.
This trial only came to an end in 1820 thanks to a money sacrifice by Vandamme.
Quelques aspects de l'enseignement artistique dans le Nord au début de la Troisième République
Authors: Lussien-Maisonneuve Marie-Josèphe 
Published: October 2015

Het onderzoek van het archief van de Schone Kunsten (reeks T) in de Archives départementales du Nord draagt bij tot de kennis van het kunstonderwijs in het Noorden in het begin van de Derde Republiek.
De vermaarde Ecoles Académiques van Rijsel, Douai, Valenciennes en Cambrai, die al voor de Revolutie bestonden, en die van Tourcoing en de Ecole spéciale van Roubaix, meer récente scheppingen, bieden een vorming die die van de Ecole des Beaux-Arts nabootst. Zij vormen ook talrijke leerlingen in de industriële en decoratieve kunsten.
De gemeentelijke scholeh van Belle, Le Cateau, Anzin, Avesnes en Maubeugebieden ook een degelijke vorming in « industriëel tekenen » (drie jaar), bestemd voor de kinderen van arbeiders en ambachtslui. Het onderwijs in de decoratieve en industriële kunsten krijgt steeds meer belang en vormt de gespecialiseerde arbeiders en ambachtslui die met hun « bel ouvrage » bijdragen tot de bouw van het regionaal patrimonium en tot de faam van de textiel — en metaalverwerkende industrie en de openbare werken.
Vicariance, long‐distance dispersal, and regional extinction–recolonization dynamics explain the disjunct circumpolar distribution of the arctic‐alpine plant Silene acaulis
Authors: Gussarova Galina , Allen Geraldine A. , Mikhaylova Yulia , McCormick Laurie J. , Mirré Virginia , Marr Kendrick L. , Hebda Richard J. , Brochmann Christian 
Published: October 2015

PREMISE OF THE STUDY:Many arctic‐alpine species have vast geographic ranges, but these may encompass substantial gaps whose origins are poorly understood. Here we address the phylogeographic history of Silene acaulis, a perennial cushion plant with a circumpolar distribution except for a large gap in Siberia.METHODS:We assessed genetic variation in a range‐wide sample of 103 populations using plastid DNA (pDNA) sequences and AFLPs (amplified fragment length polymorphisms). We constructed a haplotype network and performed Bayesian phylogenetic analyses based on plastid sequences. We visualized AFLP patterns using principal coordinate analysis, identified genetic groups using the program structure, and estimated genetic diversity and rarity indices by geographic region.KEY RESULTS:The history of the main pDNA lineages was estimated to span several glaciations. AFLP data revealed a distinct division between Beringia/North America and Europe/East Greenland. These two regions shared only one of 17 pDNA haplotypes. Populations on opposite sides of the Siberian range gap (Ural Mountains and Chukotka) were genetically distinct and appear to have resulted from postglacial leading‐edge colonizations. We inferred two refugia in North America (Beringia and the southern Rocky Mountains) and two in Europe (central‐southern Europe and northern Europe/East Greenland). Patterns in the East Atlantic region suggested transoceanic long‐distance dispersal events.CONCLUSIONS:Silene acaulis has a highly dynamic history characterized by vicariance, regional extinction, and recolonization, with persistence in at least four refugia. Long‐distance dispersal explains patterns across the Atlantic Ocean, but we found no evidence of dispersal across the Siberian range gap.