bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of LRP1 Promotes Hepatocellular Carcinoma Progression via UFL1‐Mediated Activation of NF‐κB Signaling
Authors: Guo Xingxian , Yang Fan , Liu Tianyi , Chen Amei , Liu Dina , Pu Jiangxia , Jia Can , Wu Yuanhong , Yuan Junfeng , Ouyang Nan , Herz Joachim , Ding Yinyuan 
Published: October 2024

AbstractLow‐density lipoprotein receptor‐related protein‐1 (LRP1) is thought to be correlated with hepatocellular carcinoma (HCC) invasion and metastasis. However, the precise mechanism through which LRP1 contributes to HCC progression remains unclear. Here, lower LRP1 levels are associated with malignant progression, and poor prognosis in patients with HCC is shown. LRP1 knockdown enhances the tumorigenicity of HCC cells in vitro and in vivo, whereas overexpression of either LRP1 or its β‐chain has the opposite effect. Mechanistically, LRP1 knockdown promotes the binding of ubiquitin‐like modifier 1 ligating enzyme 1 (UFL1) to OGA and accelerates ubiquitin‐mediated OGA degradation, leading to increased O‐GlcNAcylation of nuclear factor‐kappa B (NF‐κB) and subsequent inhibition of pro‐apoptotic gene expression. Conversely, exogenously expressed truncated β‐chain (β∆) stabilizes OGA by disrupting the association between UFL1 and OGA, consequently abolishing the anti‐apoptotic effects of O‐GlcNAcylated NF‐κB. The findings identify LRP1, particularly its β‐chain, as a novel upstream control factor that facilitates the stabilization of the OGA protein, thereby suppressing NF‐κB signaling and attenuating HCC progression, thus suggesting a novel therapeutic strategy for HCC.
Charge Separation Induced by Asymmetric Surface Charge Effects in Quasi‐Solid State Electrolyte for Sustainable Anion Storage
Authors: Kim Sungho , Kim Dongjoo , Kim Youngbi , Yang Heejae , Lee Hojung , Kang Jieun , Han Jihoon , Kim Youn Soo , Ryu Jaegeon , Han Jeong Woo , Park Soojin 
Published: October 2024

AbstractCompared with conventional lithium‐ion battery systems, anion‐intercalation in graphite cathodes opens avenues for the development of batteries with groundbreaking power density. This study explores the enhancement of dual‐ion batteries (DIBs) by gel polymer electrolyte (GPE) enhanced with surface‐charged nanoclays, focusing on overcoming traditional challenges such as electrolyte decomposition, anion‐solvent co‐intercalation, and interfacial instability at the graphite cathode. Three nanoclays including montmorillonite, kaolinite, and halloysite are compared by incorporating them into GPE and evaluating their effect on the electrochemical properties, ion conduction, and mechanical integrity of DIBs. Particular attention is paid to the halloysite because of its differential internal and external surface charges, which generate charge separation, facilitate optimal lithium‐ion transport, and mitigate undesirable anion‐solvent interactions. These results indicate that halloysite‐incorporated GPE (HS‐G) significantly improves DIB performance, as demonstrated by extended cycle life, robust capacity retention at fast charge/discharge rates of 20 C, and operational stability over a wide temperature range (0–60 °C). These improvements are attributed to the unique structural advantages of HS‐G, including effective charge separation, enhanced anion diffusion, and reduced solvent co‐intercalation, which provide an environmentally friendly and cost‐effective approach to advanced DIB applications.
Laseraktivierte, modulare Präzisionschemie von Graphen unter Verwendung von λ3‐Iodanen
Authors: Gerein Kevin , Unmu Dzujah Diyan , Yu Hongde , Hauke Frank , Heine Thomas , Hirsch Andreas , Wei Tao 
Published: October 2024

AbstractDas neuartige Laserschreiben stellt eine effiziente und vielversprechende Strategie für die kovalente zweidimensionale (2D) Strukturierung von Graphen dar, bleibt aber aufgrund eines Mangels an geeigneten Reagenzien ein schwieriges Unterfangen. Wir berichten hier über einen vielseitig anwendbaren Ansatz für die kovalente Laserstrukturierung von Graphen, der trivalente organische Iodverbindungen als wirksame Reagenzien verwendet und damit das Binden einer Reihe von Funktionalitäten auf die Graphenoberfläche ermöglicht. Die relativ schwachen iodzentrierten Bindungen dieser Verbindungen werden laserinduziert gespalten und erzeugen so in situ Radikale in den bestrahlten Bereichen. Diese können im nächsten Schritt an Graphen binden und schließen somit die kovalente 2D‐Strukturierung dieses 2D‐Films ab. Die maßgeschneiderte Anbringung verschiedener funktioneller Einheiten mit unterschiedlichen elektrischen Eigenschaften sowie die thermisch reversible Bindung derer, ermöglichen eine einfache Programmierung der Oberflächeneigenschaften von Graphen. Mit dieser eleganten Methode wird der Engpass einer begrenzten Anzahl an funktionellen Gruppen, die durch das Laserschreiben auf die Graphenoberfläche aufgebracht werden können, überwunden.
Taming of the microbial beasts: Plant immunity tethers potentially pathogenic microbiota members
Authors: Entila Frederickson , Tsuda Kenichi 
Published: October 2024

AbstractPlants are in intimate association with taxonomically structured microbial communities called the plant microbiota. There is growing evidence that the plant microbiota contributes to the holistic performance and general health of plants, especially under unfavorable situations. Despite the attached benefits, surprisingly, the plant microbiota in nature also includes potentially pathogenic strains, signifying that the plant hosts have tight control over these microbes. Despite the conceivable role of plant immunity in regulating its microbiota, we lack a complete understanding of its role in governing the assembly, maintenance, and function of the plant microbiota. Here, we highlight the recent progress on the mechanistic relevance of host immunity in orchestrating plant‐microbiota dialogues and discuss the pluses and perils of these microbial assemblies.
Confirmatory Adaptive Designs for Clinical Trials With Multiple Time‐to‐Event Outcomes in Multi‐state Markov Models
Authors: Danzer Moritz Fabian , Faldum Andreas , Simon Thorsten , Hero Barbara , Schmidt Rene 
Published: October 2024

ABSTRACTThe analysis of multiple time‐to‐event outcomes in a randomized controlled clinical trial can be accomplished with existing methods. However, depending on the characteristics of the disease under investigation and the circumstances in which the study is planned, it may be of interest to conduct interim analyses and adapt the study design if necessary. Due to the expected dependency of the endpoints, the full available information on the involved endpoints may not be used for this purpose. We suggest a solution to this problem by embedding the endpoints in a multistate model. If this model is Markovian, it is possible to take the disease history of the patients into account and allow for data‐dependent design adaptations. To this end, we introduce a flexible test procedure for a variety of applications, but are particularly concerned with the simultaneous consideration of progression‐free survival (PFS) and overall survival (OS). This setting is of key interest in oncological trials. We conduct simulation studies to determine the properties for small sample sizes and demonstrate an application based on data from the NB2004‐HR study.
A Fluorescent Probe Containing Triphenylamine‐Benzofuranone Moiety for Detecting Cysteine and Imaging in Living Pulmonary Cells Under Hypercapnia
Authors: Wu Xiao‐Ping , Yang Na , Liu Qing‐Qing , Zhu Zhong‐Quan 
Published: October 2024

ABSTRACTIn this work, a fluorescent probe TPB‐Cys derived from triphenylamine‐benzofuranone was developed for monitoring the cysteine (Cys) level and imaging in living pulmonary cells under hypercapnia condition. A systematic hypoxia module was tried to cover both the in‐solution test and pulmonary cellular imaging. The hypoxia condition did not obviously affect the fluorescence response signal during the in‐solution test. The fluorescence signal at 540 enhanced in a dose‐dependent enhancement along with the increase of the Cys concentration. The probe showed the advantages including relatively long linear range, high sensitivity, high stability, and high selectivity. With low cyto‐toxicity, TPB‐Cys achieved the monitoring of the endogenous Cys level in living pulmonary cells. Furthermore, it also realized the visualization of the affection of the hypoxia and hypoxia recovered conditions. This work was informative for both the accurate diagnosis and potential medical techniques.
Cost‐effective synthesis of unsymmetric tetrazines
Authors: Hwang Jiwon , Yoon Byeongjip , Suh Sung‐Eun 
Published: October 2024

Abstract1,2,4,5‐Tetrazines serve as versatile two‐carbon synthons, essential for synthesizing a variety of (hetero)aromatic compounds. Their specific reactivity with certain dienophiles, without interfering with biochemical processes, makes them ideal for bioorthogonal ligation. Despite their broad utility, current synthetic methods are costly and raise safety concerns, particularly during scale‐up. This study introduces safer and more cost‐effective synthetic strategies utilizing zinc chloride or boron trifluoride diethyl etherate as catalysts. These alternatives significantly reduce costs and enhance safety, potentially expanding the applicability of tetrazine synthesis across broader research domains.
The Role of Sex and Other Personal Characteristics in the Effects of Symptoms Severity on Self‐Care Agency in Individuals with Multiple Sclerosis
Authors: Demirci Pınar Yeşil , Yeşilot Saliha Bozdoğan , Eskimez Zehra 
Published: October 2024

ABSTRACTBackgroundMultiple sclerosis (MS) is a chronic, autoimmune disease that attacks the central nervous system.AimsThe study was conducted to determine the role of sex and other personal characteristics in the impact of symptom severity on self‐care in individuals with MS.MethodsThe study was cross‐sectional and was completed with 200 participants throughout Turkey. The data were collected through random and snowball sampling using the MS‐related symptom checklist (MS‐RS), the Fatigue Severity Scale (FSS), and the Exercise of Self‐Care Agency (ESCA) form. The data obtained were analyzed using the SPSS 21. Statistical significance was evaluated at the level of p < 0.05.ResultsThe mean age of the participants was 37.97 ± 10.6 years. The mean scores were 47.4 ± 22.41 for MS‐RS, 4.58 ± 2.2 for FSS, and 94.65 ± 24.76 for ESCA in females. The mean MS‐RS score in males was 45 ± 25.89, FSS was 4.33 ± 2.5, and ESCA was 83.43 ± 23.95. There were no significant differences between the sexes except that the ESCA scores were higher in females (p < 0.05). According to a multiple linear regression analysis, the duration of diagnosis and sensory subdimension of MS‐RS negatively affected the ESCA score in females, and this statistically significant model explained 22.6% of ESCA scores.ConclusionThe study found that both sexes had clinically significant fatigue, mildly severe symptoms, and moderate self‐care agency. While being a female positively affected self‐care agency, disease duration, and sensory symptoms negatively affected females’ self‐care agency.
Synthesis and Biological Evaluation of Isoaurone Derivatives as Anti‐inflammatory Agents
Authors: Bai Xueqian , Ye Chao , Liu Zhe , Zhou Zhijiang , Zhang Tianyi 
Published: October 2024

AbstractTwenty‐eight isoaurone derivatives with 1,2,4‐triazole moieties were synthesized using a fragment‐based design strategy, and their anti‐inflammatory activity was investigated. The anti‐inflammatory effect of the most active derivative, 14e (41.82 %), was dose‐dependent and higher than the values for celecoxib (31.82 %). Compound 14e was almost non‐toxic and inhibited different concentrations of nitric oxide (NO). The western blotting results demonstrated that cyclooxygenase‐2 (COX‐2) expression was elevated when the macrophages were exclusively treated with LPS. However, compound 14e effectively suppressed the LPS‐induced COX‐2 upregulation. Subsequent investigation revealed that 14e is a promising compound capable of inhibiting the downstream signaling of COX‐2. With the above interesting biological profile, molecular 14e could be a promising lead to develop novel anti‐inflammatory agents.
Polycyclic Polyprenylated Acylphloroglucinols (PPAPs) from Garcinia Oblongifolia and their Antitumor Activity
Authors: Yang Si‐Yu , Lin Qiang , Zhuo Xue‐Fang , Huang Yue‐Hao , Wu Wei , Wu Tian‐Yuan , Zheng Wei‐Xuan , Li Yao‐Lan , Wang Guo‐Cai , Zhang Yu‐Bo 
Published: October 2024

AbstractTwo new polycyclic polyprenylated acylphloroglucinols, along with four previously known compounds, were isolated and identified from the fruits of Garcinia oblongifolia. The structures of these compounds were elucidated through a combination of spectroscopic techniques, including MS, UV, IR, and 1D/2D NMR, as well as their chemical properties. Additionally, the cytotoxic activities of compounds 1−6 against the H134B cell line were evaluated using the MTT assay, revealing that compounds 1 and 2 exhibit promising antitumor activity.
Primary hyperaldosteronism secondary to right adrenal hyperplasia with non‐functioning left adrenal adenoma elucidated by adrenal vein sampling: A case report with in‐depth physiological review on utility of aldosterone renin ratio
Authors: Tuchinsky Adam , Sardar Sundus , Akkari Abdel‐Rauof M. , Matarneh Ahmad Samir , John Gia Susan , Ghahramani Nasrollah , Farooq Umar 
Published: October 2024

Key Clinical MessageIn cases of primary hyperaldosteronism, the aldosterone renin ratio (ARR) is crucial for determining the need for adrenal vein sampling (AVS). Our case highlights the importance of utilizing ARR with AVS, contextual interpretation of plasma renin and aldosterone levels, and stresses that sole reliance on imaging without AVS may result in unnecessary or contralateral adrenalectomy.
Growers, consultants, and county agents perceive white‐tailed deer to be the most economically impactful pest of Georgia cotton
Authors: Hand Lavesta C. , Roberts Phillip M. , Taylor Sally 
Published: October 2024

Plain Language SummaryWhite‐tailed deer damage is a common occurrence for cotton producers in the Southeastern United States, but in 2023, deer feeding on cotton and other crops was abnormally high. To determine how bad the perceived white‐tailed deer problem is in cotton, producers, county extension agents, and crop consultants were surveyed. Growers, county agents, and consultants agree that white‐tailed deer are an economic pest of cotton. The same groups reported that 33% to 41% of acres are affected by deer annually, while 34% to 42% yield loss is observed on affected acres. Additionally, $51 to $73 per acre are spent on mitigation measures according to growers, county agents, and consultants in an attempt to reduce deer damage. According to these answers, all three groups perceive white‐tailed deer to be the biggest pest of cotton compared to other common, problematic pests. This paper is the first to demonstrate the severity of this issue in cotton and serves as a starting point for research on deer damage in cotton.
Design and Analysis of a Novel Bidirectional DC‐DC Converter With Ultra‐Conversion Ratio and Reduced Current Stress
Authors: Dharavath Anusha , Pradabane Srinivasan 
Published: October 2024

ABSTRACTConventional nonisolated bidirectional DC‐DC converters are limited because of their low conversion voltage ratio. To overcome the limitation, this paper proposed a new bidirectional DC‐DC converter to introduce the novel regenerative configuration merged with the switched inductor technique for high voltage conversion applications. This design enables extreme DC‐voltage conversion with a minimal number of semiconductors and passive components. As a result, the converter's cost is reduced, and it can attain a high voltage gain with less duty ratio. In general, ultra‐gain converters suffer from high current stress across the input and output side switches in the step‐up and step‐down modes. To reduce high current stress, an active network configuration is imposed in the proposed converter. This paper describes the operating principle, steady‐state analysis of the voltage gain, and presents the performance matrices of the proposed converter. Furthermore, a comparative analysis is made with conventional and recent literature converters. Finally, a 350‐V, 500‐W, and 20‐kHz experimental prototype has been fabricated to validate the operation and corroborate the theoretical waveforms with experimental results.
Suspended Stir Bar Sorptive Extraction Based on Zr‐MOF‐Modified Cotton Thread for Enrichment and Detection of Three Trace Quinolones in Fish Tissue
Authors: Long Jiao , Gao Yan , Sheng Kangjia , Bao Tao , Wang Sicen 
Published: October 2024

ABSTRACTIn this study, an efficient and practical method was established to enrich and detect three trace quinolones in fish tissue. Low‐cost and environment‐friendly cotton threads were coated with PCN‐224 using a simple one‐pot hydrothermal method. The PCN‐224‐modified cotton threads (PCN‐224@CTs) were characterized to ensure that the crystal was successfully fabricated on the surface of cotton threads. The prepared PCN‐224@CTs were wrapped around steel needles to form suspended bars. Key factors of extraction and desorption were optimized, and analytical performance was tested. Suspended bar sorptive extraction coupled with ultra‐performance liquid chromatography (UPLC) with a photodiode array detector was used to analyze the quinolones in fish tissue. The detection limits of quinolones were 0.26–1.15 ng/mL, and the recoveries were achieved in the range of 81.31%–115.81%, indicating a simple method with high sensitivity and satisfactory practicability of enriching, separating, and detecting trace quinolones was successfully established.
Development of the SupersonicIEF Method for High‐Throughput Charge Variant Analysis
Authors: McElroy Will , Heger Christopher D. 
Published: October 2024

ABSTRACTThe analysis of biopharmaceuticals for charge variants occurs from early‐stage samples through formulation and process‐development optimization. Higher throughput methods allow increased analysis of these samples to facilitate greater understanding of the samples and to better optimize their production and formulation. To enable higher throughput charge variant analysis, a new, rapid platform imaged capillary isoelectric focusing (icIEF) method was optimized to be two to three times faster than standard methods.
Applications of Probabilistic Genotyping Method for Combining Evidence Across Microhaplotype DNA Mixture Profiles
Authors: Ling Xiaohua , Han Shuang , Lin Xinyi , Bai Zhaochen , Zhang Nan , Li Jiayue , Wang Huan , Ou Xueling 
Published: October 2024

ABSTRACTIn cases of serious crimes that involve challenging DNA samples from the perpetrator (e.g., a minor contributor to a mixture), there is justification to combine different mixture profiles. In our previous study, we developed a massively parallel sequencing (MPS)–based assay targeting 140 microhaplotype markers. In this study, we extended the use of the microhaplotype panel to common scenarios, such as determining the presence of a common contributor or relatedness between different mixture profiles when no reference source is available. Data interpretation was performed using the R package KinMix. Our findings revealed that correct assignments of a common contributor and relatedness were made between relatively balanced mixtures. However, when profiles suffered from allele imbalance, inclusive assignments were significantly associated with the suspect's mixture proportion. Additionally, our analysis showed zero false‐positive rates in the studied scenarios. These results indicate that microhaplotype data can be reliably interpreted for identifying a common donor or related donors among different mixtures. Further research based on larger sample sizes may yield more reliable results, which could assist in solving issues related to complex scenarios where multiple mixed profiles were involved.
Simultaneous Genotyping of Three SNVs, rs5471, rs5472, and rs2000999 Involved in Serum Haptoglobin Levels by Fluorescent Probe‐Based Melting Curve Analysis
Authors: Soejima Mikiko , Koda Yoshiro 
Published: October 2024

ABSTRACTHaptoglobin (Hp) is a hemoglobin‐binding acute‐phase serum protein. Several single nucleotide variations (SNVs) within the Hp gene (HP) or Hp‐related protein gene (HPR), such as rs5471 (A > C) and rs5472 (A > G) in HP promoter region and rs2000999 (G > A) in intron 2 of HRP, are suggested to correlate with the serum Hp levels. To determine these three SNVs simultaneously, a genotyping assay based on duplex dual‐labeled fluorescent probes was developed. The method was then validated by analyzing genomic DNA from 121 Ghanaian and two Japanese subjects who had been previously genotyped for rs5471, rs5472, and rs2000999. Both rs5471 and rs5472 could be determined as haplotypes with a single FAM‐labeled fluorescent probe, and rs2000999 could be genotyped with a HEX‐labeled fluorescent probe. The results obtained with the present method were consistent with the previous results except for those of three Ghanaian subjects. All three subjects appear to have multiple HPR copy number variants characteristic of African populations, which may have led to incorrect results during previous genotyping. This method allows us to genotype these three SNVs in a relatively large number of samples, especially in African populations where rs5471 is uniquely distributed.
Determination of 40K radionuclide concentration and elements content in some animal feeds
Authors: Eke Canel 
Published: October 2024

AbstractThe study of radionuclide concentrations is significant for the exposure of the radiation on the livings from these radionuclides and they are transferred from plants, animals to livings, and this is important for the health of livings. In this paper, 40K concentration and elements content of wheat, barley, corn, and chick feeds, which are used as animal feed using gamma‐ray spectrometry and inductively coupled plasma‐optical emission spectrometry (ICP‐OES) were examined. The animal feeds were obtained from two different sellers for representative and to be able to compare the obtained results from studied animal feeds. The mean value of 40K radionuclide concentration for wheat, barley, and corn is smaller than the world mean value whereas it is higher for chick feeds than the world mean value. The Hin, Hex, and Iγ of studied animal feeds are lower than limit value reported in the scientific report. There are positive and significant correlations between K, Ca, Fe, Mg, Na, P, and 40K at the 0.01 level and between Cu, Mn, Ni, Zn, and 40K at 0.05 level.