bluuebunny/crossref_metadata_2025_split huggingface dataset:
Show me your neighbours, and I’ll tell you what you are – cellular microenvironment matters
Authors: Toth Timea , Balassa Tamas , Bara Norbert , Kovacs Ferenc , Kriston Andras , Molnar Csaba , Haracska Lajos , Sukosd Farkas , Horvath Peter 
Published: December 2017

AbstractTo answer major questions of cell biology, it is essential to understand cellular complexity. Modern automated microscopes produce vast amounts of images routinely, making manual analysis nearly impossible. Due to their efficiency, machine learning-based analysis software have become essential tools to perform single-cell-level phenotypic analysis of large imaging datasets. However, an important limitation of such methods is that they do not use the information gained from the cellular micro- and macroenvironment: the algorithmic decision is based solely on the local properties of the cell of interest. Here, we present how various microenvironmental features contribute to identifying a cell and how such additional information can improve single-cell-level phenotypic image analysis. The proposed methodology was tested for different sizes of Euclidean and nearest neighbour-based cellular environments both on tissue sections and cell cultures. Our experimental data verify that the microenvironment of a cell largely determines its entity. This effect was found to be especially strong for established tissues, while it was somewhat weaker in the case of cell cultures. Our analysis shows that combining local cellular features with the properties of the cell's microenvironment significantly improves the accuracy of machine learning-based phenotyping.
Increased microglial synapse elimination in patient-specific models of schizophrenia
Authors: Sellgren Carl M. , Gracias Jessica , Watmuff Bradley , Goold Carleton P. , Thanos Jessica M. , Fu Ting , Karmacharya Rakesh , Brown Hannah E. , Wang Jennifer , Sheridan Steven D. , Perlis Roy H. 
Published: December 2017

AbstractSchizophrenia patients display decreased synaptic density in postmortem studies, suggesting aberrant microglial synapse elimination during neurodevelopment. Here, we use cellular reprogramming to create patient-specific in vitro models of microglia-mediated synapse engulfment that demonstrate increased synapse elimination in schizophrenia-derived models compared to healthy controls. We show that excessive synaptic pruning in schizophrenia reflects abnormalities in microglia-like cells as well as synaptic structures. Further, we find that schizophrenia risk-associated variants within the complement component 4 locus contribute to the increased uptake in schizophrenia models. Finally, we demonstrate that the antibiotic minocycline reduces microglia-mediated synapse uptake and show that minocycline treatment for acne is associated with a reduction in incident schizophrenia risk compared to other treatments in a cohort of more than 9,000 young adults drawn from health records. Specific pharmacological interventions targeting excessive pruning merit further study for their capacity to delay or prevent the onset of schizophrenia in high-risk individuals.
Measuring mosquito-borne viral suitability in Myanmar and implications for local Zika virus transmission
Authors: Perez PN , Obolski U , Alcantara LCJ , Maia de Lima M , Ashley EA , Smithuis F , Horby P , Maude RJ , Zaw L , Kyaw AM , Lourenço J 
Published: December 2017

AbstractINTRODUCTION: In South East Asia, mosquito-borne viruses (MBVs) have long been a cause of high disease burden and significant economic costs. While in some SEA countries the epidemiology of MBVs is spatio-temporally well characterised and understood, in others such as Myanmar our understanding is largely incomplete. MATERIALS AND METHODS: Here, we use a simple mathematical approach to estimate a climate-driven suitability index aiming to better characterise the intrinsic, spatio-temporal potential of MBVs in Myanmar. RESULTS: Results show that the timing and amplitude of the natural oscillations of our suitability index are highly informative for the temporal patterns of DENV case counts at the country level, and a mosquito-abundance measure at a city level. When projected at fine spatial scales, the suitability index suggests that the time period of highest MBV transmission potential is between June and October independently of geographical location. Higher potential is nonetheless found along the middle axis of the country and in particular in the southern corridor of international borders with Thailand. DISCUSSION: This research complements and expands our current understanding of MBV transmission potential in Myanmar, by identifying key spatial heterogeneities and temporal windows of importance for surveillance and control. We discuss our findings in the context of Zika virus given its recent worldwide emergence, public health impact, and current lack of information on its epidemiology and transmission potential in Myanmar. The proposed suitability index here demonstrated is applicable to other regions of the world for which surveillance data is missing, either due to lack of resources or absence of an MBV of interest.
Feature-specific awake reactivation in human V1 after visual training
Authors: Bang Ji Won , Sasaki Yuka , Watanabe Takeo , Rahnev Dobromir 
Published: December 2017

AbstractConverging human studies have demonstrated that brain activity patterns observed during task performance reemerge in the following restful awake state. Such “awake reactivation” has been demonstrated across higher-order cortex for complex images or associations. However, it remains unclear what specific training components are reactivated in these studies. Here we sought to provide evidence for the reactivation of a particular visual feature – Gabor orientation. Following extensive training on a visual task, we found robust reactivation in human V1 that lasted at least eight minutes. This effect was not present in higher retinotopic areas such as V2, V3, V3A, or V4v, demonstrating that the effects in V1 are not due to top-down processes such as conscious rehearsal. Furthermore, the amount of awake reactivation predicted the amount of performance improvement on the visual task. These results demonstrate that functionally-relevant awake reactivation of specific visual features occurs in early sensory cortex.
Identification of Gene Regulation Models from Single-Cell Data
Authors: Weber Lisa , Raymond William , Munsky Brian 
Published: December 2017

AbstractIn quantitative analyses of biological processes, one may use many different scales of models (e.g., spatial or non-spatial, deterministic or stochastic, time-varying or at steady-state) or many different approaches to match models to experimental data (e.g., model fitting or parameter uncertainty/sloppiness quantification with different experiment designs). These different analyses can lead to surprisingly different results, even when applied to the same data and the same model. We use a simplified gene regulation model to illustrate many of these concerns, especially for ODE analyses of deterministic processes, chemical master equation and finite state projection analyses of heterogeneous processes, and stochastic simulations. For each analysis, we employ Matlab and Python software to consider a time-dependent input signal (e.g., a kinase nuclear translocation) and several model hypotheses, along with simulated single-cell data. We illustrate different approaches (e.g., deterministic and stochastic) to identify the mechanisms and parameters of the same model from the same simulated data. For each approach, we explore how uncertainty in parameter space varies with respect to the chosen analysis approach or specific experiment design. We conclude with a discussion of how our simulated results relate to the integration of experimental and computational investigations to explore signal-activated gene expression models in yeast [1] and human cells [2]‡.PACS numbers: 87.10.+e, 87.15.Aa, 05.10.Gg, 05.40.Ca,02.50.-rSubmitted to: Phys. Biol.
Shallow whole genome sequencing for robust copy number profiling of formalin-fixed paraffin-embedded breast cancers
Authors: Chin Suet Feung , Santoja Angela , Grzelak Marta , Ahn Soomin , Sammut Stephen-John , Clifford Harry , Rueda Oscar M. , Pugh Michelle , Goldgraben Mae A. , Bardwell Helen A. , Cho Eun Yoon , Provenzano Elena , Rojo Federico , Alba Emilio , Caldas Carlos 
Published: December 2017

ABSTRACTPathology archives with linked clinical data are an invaluable resource for translational research, with the limitation that most cancer samples are formalin-fixed paraffin-embedded (FFPE) tissues. Therefore, FFPE tissues are an important resource for genomic profiling studies but are under-utilised due to the low amount and quality of extracted nucleic acids. We profiled the copy number landscape of 356 breast cancer patients using DNA extracted FFPE tissues by shallow whole genome sequencing. We generated a total of 491 sequencing libraries from 2 kits and obtained data from 98.4% of libraries with 86.4% being of good quality. We generated libraries from as low as 3.8ng of input DNA and found that the success was independent of input DNA amount and quality, processing site and age of the fixed tissues. Since copy number alterations (CNA) play a major role in breast cancer, it is imperative that we are able to use FFPE archives and we have shown in this study that sWGS is a robust method to do such profiling.
Single-cell characterization of step-wise acquisition of carboplatin resistance in ovarian cancer
Authors: Wenzel Alexander T. , Champa Devora , Venkatesh Hrishi , Sun Si , Tsai Cheng-Yu , Mesirov Jill P. , Bui Jack D. , Howell Stephen B. , Harismendy Olivier 
Published: December 2017

AbstractAcquired resistance to carboplatin is a major obstacle to the cure of ovarian cancer, but its molecular underpinnings are still poorly understood and often inconsistent between in vitro modeling studies. Using sequential treatment cycles, multiple clones derived from a single ovarian cancer cell reached similar levels of resistance. The resistant clones showed significant transcriptional heterogeneity, with shared repression of cell cycle processes and induction of IFNα response signaling, and subsequent pharmacological inhibition of the JAK/STAT pathway led to a general increase in carboplatin sensitivity. Gene-expression based virtual synchronization of 26,772 single cells from 2 treatment steps and 4 resistant clones was used to evaluate the activity of Hallmark gene sets in proliferative (P) and quiescent (Q) phases. Two behaviors were associated with resistance: 1) broad repression in the P phase observed in all clones in early resistant steps and 2) prevalent induction in Q phase observed in the late treatment step of one clone. Furthermore, the induction of IFNα response in P phase or Wnt-signaling in Q phase were observed in distinct resistant clones. These observations suggest a model of resistance hysteresis, where functional alterations of the P and Q phase states affect the dynamics of the successive transitions between drug exposure and recovery, and prompts for a precise monitoring of single-cell states to develop more effective schedules for, or combination of, chemotherapy treatments.
High resolution in situ structural determination of heterogeneous specimen
Authors: Himes Benjamin A. , Zhang Peijun 
Published: December 2017

AbstractMacromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single particle cryoEM. Such complexes may be studied in situ using cryo-electron tomography combined with sub-tomogram alignment and classification, which in exceptional cases reaches sub-nanometer resolution, yielding insight into structure-function relationships. All maps currently deposited in the EMDB with resolution < 9 Å are from macromolecules that form ordered structural arrays, like viral capsids, which greatly simplifies structural determination. Extending this approach to more common specimens that exhibit conformational or compositional heterogeneity, and may be available in limited numbers, remains challenging. We developed emClarity, a GPU-accelerated image processing package, specifically to address fundamental hurdles to this aim, and demonstrate significant improvements in the resolution of maps compared to those generated using current state-of-the-art software. Furthermore, we devise a novel approach to sub-tomogram classification that reveals functional states not previously observed with the same data.The software is freely available from https://www.github.com/bHimes/emClarityTutorial documentation and videos at https://www.github.com/bHimes/emClarity/wiki
Burly1is a mouse QTL for lean body mass that maps to a 0.8-Mb region on chromosome 2
Authors: Lin Cailu , Fesi Brad D. , Marquis Michael , Bosak Natalia P. , Lysenko Anna , Koshnevisan Mohammed Amin , Duke Fujiko F. , Theodorides Maria L. , Nelson Theodore M. , McDaniel Amanda H. , Avigdor Mauricio , Arayata Charles J. , Shaw Lauren , Bachmanov Alexander A. , Reed Danielle R. 
Published: December 2017

AbstractOur goal was to fine map a mouse QTL for lean body mass (Burly1) using information from several populations including newly created congenic mice derived from the B6 (host) and 129 (donor) strains. The results from each mapping population were concordant and showed thatBurly1is likely a single QTL in a 0.8-Mb region at 151.9-152.7 Mb (rs33197365tors3700604) on mouse chromosome 2. Results from mice of all the mapping populations we studied including intercrossed, backcrossed, consomic, and congenic strains indicate that lean body mass was increased by the B6-derived allele relative to the 129-derived allele. We determined that the congenic region harboringBurly1contains 26 protein-coding genes, 11 noncoding RNA elements (e.g., lncRNA), and 4 pseudogenes, with 1949 predicted functional variants. The effect of theBurly1locus on lean body weight was apparent at all ages measured and did not affect food intake or locomotor activity. However, congenic mice with the B6-allele produced more heat per kilogram of lean body weight than did controls, pointing to a genotype effect on lean mass metabolism. These results show the value of integrating information from several mapping populations to refine the map location of body composition QTLs.
Empathy and affect in B2B salesperson performance
Authors: Anaza Nwamaka A. , Inyang Aniefre Eddie , Saavedra Jose L. 
Published: December 2017

Purpose
The purpose of this study is to explore salesperson empathy and the moderating impact of positive/negative affect on a salesperson’s listening and adaptive selling behaviors. It also seeks to identify whether and how empathy influences performance.

Design/methodology/approach
The study’s hypothesis was analyzed using data collected from business-to-business salespeople working for a manufacturing firm. A partial least squares analysis was used to test the study’s proposed hypotheses.

Findings
The results of this study show that empathy and the moderating role of positive affect foster desirable sales behaviors (listening and adaptive selling behaviors) that subsequently enhance in-role (expected) and extra-role (discretionary) performance.

Originality/value
Contributions from the findings enhance the literature through its consideration of how the direct effect of empathy on sales behaviors (a salesperson’s listening and adapting selling behavior) is moderated by the salesperson’s positive and negative affect and how sales behaviors impact final sales outcomes (in-role and extra-role performance).
Emotion in sales performance: affective orientation and need for cognition and the mediating role of motivation to work
Authors: Nowlin Edward , Walker Doug , Deeter-Schmelz Dawn R. , Haas Alexander 
Published: December 2017

PurposeThe purpose of this paper is to investigate whether and under what condition does affective orientation (AO) drive salesperson performance (SP) and whether there is a tradeoff between affective orientation and the need for cognition (NFC). Using career stage theory, this research proposes that emotion is important and that the relationship between AO and SP is conditional and mediated.Design/methodology/approachThe hypothesized model is tested using survey data that were collected from 611 attendees at a Midwest regional sales meeting of a national direct selling organization. The model was estimated using 5,000 bootstrapped samples drawn to assess the conditional and indirect effects.FindingsThe findings reveal that AO increases SP when mediated through motivation to work (MW), but only during the salesperson’s initial stage of their career – their first year. In subsequent career stages, AO’s impact on SP diminishes, while NFC’s impact on SP remains significant regardless of career stage.Research limitations/implicationsThe data were collected from a single selling organization.Practical implicationsThis study increases the understanding of the relationship between salesperson emotion (AO) and SP. This informs sales managers that new salespeople interpret information both emotionally and cognitively, which impacts the management of early career salespeople.Originality/valueSales research rarely investigates the role of emotion. This research finds that emotion can be an asset to new salespeople. However, the need for emotion (AO) decreases with experience and no longer has a significant impact on performance after the initial stage.
Triggering and tempering brand advocacy by frontline employees: vendor and customer-related influences
Authors: Badrinarayanan Vishag , Sierra Jeremy J. 
Published: December 2017

Purpose
Lawler (2001) posits that social exchanges create a sense of shared responsibility for outcome success. The purpose of this study is to apply this framework to the vendor/frontline employee/customer triad to examine the underlying role of emotions in how frontline employees’ evaluations of vendors and customers trigger and temper brand advocacy efforts, respectively.

Design/methodology/approach
With cross-sectional data from 168 frontline employees working at a leading national retailer of electronic goods, path analysis is used to evaluate the hypotheses.

Findings
Frontline employees’ relationship quality with the vendor and perceptions of vendors’ product quality positively influence brand advocacy. Also, customers’ brand affinity and recommendation preference both demonstrate a significant, negative curvilinear relationship with brand advocacy.

Research limitations/implications
Frontline employees’ emotion-laden evaluations of vendors and customer influence brand advocacy in different ways. Vendor relationship quality and brand quality perceptions “trigger” brand advocacy. However, customer’s affinity toward a vendor’s brand and willingness to seek recommendations “temper” brand advocacy. Specifically, brand advocacy effort is low when customers possess very low and very high affinity toward a focal brand – moderate affinity spurs high advocacy; likewise, advocacy is low when customers demonstrate very low and very high interest in seeking the frontline employees’ opinion – moderate interest spurs high advocacy. Although ideal to examine vendor and customer emotional exchanges, using only frontline employee data from a technology-selling retailer may constrain generalizability.

Practical implications
Frontline employee training programs should emphasize the customer’s role in the transaction to increase perceptions of shared responsibility, as a means to create a favorable emotional experience, and accentuate timing strategies on when to pursue heightened or diminished emotionally charged brand advocacy efforts.

Originality/value
This study contributes to the frontline employee behavior literature by viewing shared responsibility in transactions as a source of emotional value, explaining variance in frontline employee brand advocacy through relationship and product quality dimensions, and uncovering curvilinear effects for customers’ brand affinity and recommendation preference in elucidating brand advocacy.
Contrasting effects of salespeople’s emotion appraisal ability in a transaction-oriented environment
Authors: Briggs Elten , Kalra Ashish , Agnihotri Raj 
Published: December 2017

PurposeAlthough the role of emotions in buyer–seller exchanges is important, it remains understudied, especially in the business-to-business selling context. This paper aims to provide insights into the role of the salespeople’s ability to appraise emotions (EA ability) and its effects on job-related outcomes in a transaction-oriented environment.Design/methodology/approachMulti-source data were gathered from 152 salespeople working for a financial service firm. Customer service and sales performance ratings were reported by supervisors. Hypotheses were tested through structural equation modeling using AMOS.FindingsThe study finds contrasting effects of EA ability on sales performance in the firm’s transaction-oriented environment. On the one hand, EA ability motivates better salesperson customer service, which then increases their sales performance. On the other hand, EA ability enhances emotional exhaustion, which detracts from sales performance.Practical implicationsSales managers should consider the ability of new hires to appraise emotions when determining their fit with the job and the organization. Training programs that develop salesperson emotional abilities should be comprehensive as it may be detrimental to be high in EA ability without the skills to regulate or use emotions.Originality valueThe study is one of the first to consider the effects of emotional abilities in the context of a transaction-oriented environment. By focusing specifically on EA ability, the study provides greater understanding of the influences of the individual components of emotional intelligence, rather than salesperson’s overall capacity for emotional intelligence.
The heart in organizational buying: marketers’ understanding of emotions and decision-making of buyers
Authors: Kemp Elyria A. , Borders Aberdeen Leila , Anaza Nwamaka A. , Johnston Wesley J. 
Published: December 2017

Purpose
Organizational buying behavior has often been treated as a rational activity, even though humans are involved in the decision-making. Human decision-making often includes a complex cadre of emotions and rationalizations. Subsequently, organizational buyers may not only be driven by logic, testing and facts, but also by emotions. The purpose of this paper is to investigate the role that emotions play in organizational buying behavior.

Design/methodology/approach
In-depth interviews were conducted with marketing decision-makers for one of the most valuable brands in the world. The role that emotions play in the behavior of organizational buyers is elucidated from the perspective of these marketing professionals.

Findings
Emotions are prevalent at all stages in the organizational decision-making process and various discrete emotions fuel action tendencies among buyers. Efforts are made by marketers to strategically manage the emotions buyers experience.

Practical implications
Although organizational buyers must see the functional value of a product or brand, companies need to consider ways in which brands can connect with buyers on an emotional and personal level.

Originality/value
This paper contributes to the literature by offering insights into which discrete or specific emotions are most prominent in organizational buying behavior and how the manifestation of these emotions impact decision-making at each stage in the buying cycle.