bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developmentally‐faithful and effective human erythropoiesis in immunodeficient and Kit mutant mice
Authors: Fiorini Claudia , Abdulhay Nour J. , McFarland Sean K. , Munschauer Mathias , Ulirsch Jacob C. , Chiarle Roberto , Sankaran Vijay G. 
Published: June 2017

AbstractImmunodeficient mouse models have been valuable for studies of human hematopoiesis, but high‐fidelity recapitulation of erythropoiesis in most xenograft recipients remains elusive. Recently developed immunodeficient and Kit mutant mice, however, have provided a suitable background to achieve higher‐level human erythropoiesis after long‐term hematopoietic engraftment. While there has been some characterization of human erythropoiesis in these models, a comprehensive analysis from various human developmental stages has not yet been reported. Here, we have utilized cell surface phenotypes, morphologic analyses, and molecular studies to fully characterize human erythropoiesis from multiple developmental stages in immunodeficient and Kit mutant mouse models following long‐term hematopoietic stem and progenitor cell engraftment. We show that human erythropoiesis in such models demonstrates complete maturation and enucleation, as well as developmentally appropriate globin gene expression. These results provide a framework for future studies to utilize this model system for interrogating disorders affecting human erythropoiesis and for developing improved therapeutic approaches.
The prognostic significance of polyclonal bone marrow plasma cells in patients with relapsing multiple myeloma
Authors: Ghosh Toshi , Gonsalves Wilson I. , Jevremovic Dragan , Dispenzieri Angela , Dingli David , Timm Michael M. , Morice William G. , Kapoor Prashant , Kourelis Taxiarchis V. , Lacy Martha Q. , Hayman Suzanne R. , Buadi Francis K. , Leung Nelson , Go Ronald S. , Lin Yi , Russell Stephen J. , Lust John A. , Zeldenrust Steven R. , Warsame Rahma , Hwa Yi L. , Kyle Robert A. , Gertz Morie A. , Vincent Rajkumar S. , Kumar Shaji K. 
Published: June 2017

AbstractPrior studies have revealed that the presence of increasing number of polyclonal plasma cells (pPCs) in the bone marrow (BM) are associated with better outcomes in newly diagnosed multiple myeloma (MM) patients. This effect has not been studied in patients with MM at the time of disease relapse. We determined the prognostic value of depletion of pPCs in the BM by 7‐color multiparameter flow cytometry in a series of 174 relapsing MM patients. The time to next therapy (TTNT) in those with <5% pPCs was 9.4 months versus 13.9 months in those with ≥5% pPCs (P = .0091). The median overall survival (OS) in those with <5% pPCs was 21.4 months, while the median OS was not reached in those patients with ≥5% pPCs (P = .019). Of the 109 patients with standard risk cytogenetics, the median OS of those with <5% pPCs was 28.4 months, while the median OS was not reached in those with ≥5% pPCs (P = .033). As such, <5% pPCs in the BM appears to have prognostic utility in identifying a subset of relapsing MM patients, even with standard‐risk cytogenetics, who have a particularly adverse outcome.
Stable Heterometallic Cluster Ions based on Werner's Hexol
Authors: Marsh David A. , Elliott William S. , Smith Rachel M. , Sharps Meredith C. , Baumeister Mary K. , Carnes Matthew E. , Zakharov Lev N. , Casey William H. , Johnson Darren W. 
Published: June 2017

AbstractLarge aqueous ions are interesting because they are useful in materials science (for example to generate thin films) but also because they serve as molecular models for the oxide–aqueous mineral interface where spectroscopy is difficult. Here we show that new clusters of the type M[(μ‐OH)2Co(NH3)4]3(NO3)6 (M=Al, Ga) can be synthesized using Werner's century‐old cluster as a substitutable framework. We substituted Group 13 metals into the hexol Co[(μ‐OH)2Co(NH3)4]36+ ion to make diamagnetic heterometallic ions. The solid‐state structure of the hexol‐type derivatives were determined by single‐crystal XRD and NMR spectroscopy and confirmed that the solid‐state structure persists in solution after dissolution into either D2O or [D6]DMSO. Other compositions besides these diamagnetic ions can undoubtedly be made using a similar approach, which considerably expands the number of stable aqueous heteronuclear ions.
Vancomycin‐Dependent Response in Live Drug‐Resistant Bacteria by Metabolic Labeling
Authors: Pidgeon Sean E. , Pires Marcos M. 
Published: June 2017

AbstractThe surge in drug‐resistant bacterial infections threatens to overburden healthcare systems worldwide. Bacterial cell walls are essential to bacteria, thus making them unique targets for the development of antibiotics. We describe a cellular reporter to directly monitor the phenotypic switch in drug‐resistant bacteria with temporal resolution. Vancomycin‐resistant enterococci (VRE) escape the bactericidal action of vancomycin by chemically modifying their cell‐wall precursors. A synthetic cell‐wall analogue was developed to hijack the biosynthetic rewiring of drug‐resistant cells in response to antibiotics. Our study provides the first in vivo VanX reporter agent that responds to cell‐wall alteration in drug‐resistant bacteria. Cellular reporters that reveal mechanisms related to antibiotic resistance can potentially have a significant impact on the fundamental understanding of cellular adaption to antibiotics.
Catalytic Asymmetric Conjugate Addition of Indolizines to α,β‐Unsaturated Ketones
Authors: Correia José Tiago Menezes , List Benjamin , Coelho Fernando 
Published: June 2017

AbstractA catalytic enantioselective conjugate addition of indolizines to enones is described. The chiral phosphoric acid (S)‐TRIP activates α,β‐unsaturated ketones, thereby promoting an enantioface‐differentiating attack by indolizines. Using this reaction, several alkylated indolizines were synthesized in good yields and with enantiomeric ratios of up to 98:2.
A Generalized Strategy for the Synthesis of Large‐Size Ultrathin Two‐Dimensional Metal Oxide Nanosheets
Authors: Zhao Hewei , Zhu Yujie , Li Fengshi , Hao Rui , Wang Shaoxiong , Guo Lin 
Published: June 2017

AbstractTwo‐dimensional (2D) nanomaterials show unique electrical, mechanical, and catalytic performance owing to their ultrahigh surface‐to‐volume ratio and quantum confinement effects. However, ways to simply synthesize 2D metal oxide nanosheets through a general and facile method is still a big challenge. Herein, we report a generalized and facile strategy to synthesize large‐size ultrathin 2D metal oxide nanosheets by using graphene oxide (GO) as a template in a wet‐chemical system. Notably, the novel strategy mainly relies on accurately controlling the balance between heterogeneous growth and nucleation of metal oxides on the surface of GO, which is independent on the individual character of the metal elements. Therefore, ultrathin nanosheets of various metal oxides, including those from both main‐group and transition elements, can be synthesized with large size. The ultrathin 2D metal oxide nanosheets also show controllable thickness and unique surface chemical state.
Europium Oxybromide Catalysts for Efficient Bromine Looping in Natural Gas Valorization
Authors: Paunović Vladimir , Lin Ronghe , Scharfe Matthias , Amrute Amol P. , Mitchell Sharon , Hauert Roland , Pérez‐Ramírez Javier 
Published: June 2017

AbstractThe industrialization of bromine‐mediated natural gas upgrading is contingent on the ability to fully recycle hydrogen bromide (HBr), which is the end form of the halogen after the activation and coupling of the alkanes. Europium oxybromide (EuOBr) is introduced as a unique catalytic material to close the bromine loop via HBr oxidation, permitting low‐temperature operation and long lifetimes with a stoichiometric feed (O2:HBr=0.25)—conditions at which any catalyst reported to date severely deactivates because of excessive bromination. Besides, EuOBr exhibits unparalleled selectivity to methyl bromide in methane oxybromination, which is an alternative route for bromine looping. This novel active phase is finely dispersed on appropriate carriers and scaled up to technical extrudates, enhancing the utilization of the europium phase while preserving the performance. This catalytic system paves the way for sustainable valorization of stranded natural gas via bromine chemistry.
Micellization of pH‐sensitive poly(butadiene)‐block‐poly(2 vinylpyridine)‐block‐poly(ethylene oxide) triblock copolymers: Complex formation with anionic surfactants
Authors: Atanase Leonard Ionut , Lerch Jean‐Philippe , Caprarescu Simona , Iurciuc (Tincu) Camelia Elena , Riess Gerard 
Published: June 2017

ABSTRACTThe micellization of three tailor‐made triblock copolymers, such as PB100–P2VP100–PEO104, PB185–P2VP108–PEO154, and PB37–P2VP115–PEO241, having similar total molecular weights and constant poly(2‐vinylpyridine) (P2VP) sequence lengths, was investigated as a function of pH and sodium dodecyl sulfate (SDS) concentration. At pH 7 the formation of intermicellar aggregates was observed, especially for copolymers of low poly(ethylene oxide) (PEO) content. A pH decrease from 7 to 3 leads to a particle size increase due to the electrostatic repulsion of the protonated P2VP chains. The influence of the PEO sequence length was also observed for zeta potential values. At pH 3, in the absence of SDS, core–shell–corona micelles are formed whereas in the presence of small amount of SDS (degree of neutralization DN = 0%–50%), a complex is formed between SDS and the protonated P2VP which leads to the shrinkage of the shell and thus to a decrease of the micellar sizes. For higher DN values, the micellar sizes increase due to the formation of large agglomerates and a transition occurs from a monomodal to a bimodal size distribution. Furthermore, it turned out that secondary aggregation, such as intermicellar aggregation, can completely be avoided if the degree of polymerization (DPn) of the water‐soluble block is significantly higher than the DPn of the water‐insoluble sequence. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45313.
BSA–AuNPs@Tb–AMP metal–organic frameworks for ratiometric fluorescence detection of DPA and Hg2+
Authors: Cai Keying , Zeng Mulan , Liu Fenfen , Liu Nan , Huang Zhenzhong , Song Yonghai , Wang Li 
Published: June 2017

AbstractAn easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA–AuNPs@Tb–AMP (BSA, bovine serum albumin; AMP, adenosine 5′‐monophosphate; AuNPs, Au nanoparticles) metal–organic framework (MOF) nanostructures were synthesized by encapsulating BSA–AuNPs into Tb–AMP MOFs for the detection of 2,6‐pyridinedicarboxylic acid (DPA) and Hg2+. DPA could strongly co‐ordinate with Tb3+ to replace water molecules from the Tb3+ center and accordingly enhanced the fluorescence of Tb–AMP MOFs. The fluorescence of BSA–AuNPs at 405 nm remained constant. While the fluorescence of BSA–AuNPs at 635 nm was quenched after Hg2+ was added, the fluorescence of Tb–AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg2+. The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F545/F405 linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F635/F405 increased linearly with increase of Hg2+ concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg2+ in running water.
Meta-analysis of single-port versus conventional laparoscopic cholecystectomy comparing body image and cosmesis
Authors: Haueter R , Schütz T , Raptis D A , Clavien P-A , Zuber M 
Published: June 2017

Abstract

Background
The purpose of this study was to evaluate improvements in cosmetic results and postoperative morbidity for single-incision laparoscopic cholecystectomy (SILC) in comparison with multiport laparoscopic cholecystectomy (MLC).

Methods
A literature search was undertaken for RCTs comparing SILC with MLC in adult patients with benign gallbladder disease. Primary outcomes were body image and cosmesis scores at different time points. Secondary outcomes included intraoperative and postoperative complications, postoperative pain and frequency of port-site hernia.

Results
Thirty-seven RCTs were included, with a total of 3051 patients. The body image score favoured SILC at all time points (short term: mean difference (MD) –2·09, P &lt; 0·001; mid term: MD −1·33, P &lt; 0·001), as did the cosmesis score (short term: MD 3·20, P &lt; 0·001; mid term: MD 4·03, P &lt; 0·001; long-term: MD 4·87, P = 0·05) and the wound satisfaction score (short term: MD 1·19, P = 0·03; mid term: MD 1·38, P &lt; 0·001; long-term: MD 1·19, P = 0·02). Duration of operation was longer for SILC (MD 13·56 min; P &lt; 0·001) and SILC required more additional ports (odds ratio (OR) 6·78; P &lt; 0·001). Postoperative pain assessed by a visual analogue scale (VAS) was lower for SILC at 12 h after operation (MD in VAS score −0·80; P = 0·007). The incisional hernia rate was higher after SILC (OR 2·50, P = 0·03). All other outcomes were similar for both groups.

Conclusion
SILC is associated with better outcomes in terms of cosmesis, body image and postoperative pain. The risk of incisional hernia is four times higher after SILC than after MLC.
Associating students and teachers for tutoring in higher education using clustering and data mining
Authors: Urbina Nájera Argelia B. , de la Calleja Jorge , Medina Ma. Auxilio 
Published: June 2017

AbstractTutoring is part of the teaching–learning process; this is considered a complementary strategy to support the development of integral and competent professionals. When teachers deal with large groups of students such as in digital learning environments, tutoring becomes a time‐consuming and difficult task that can cause distraction and overload. This paper presents an experimental study to associate students and teachers for tutoring according to their skills and affinities using the clustering methods of k‐means, expectation maximization, and farthest first. The study harvests data of 1,199 university students and 35 teachers. The results reached 100% of compatibility between clusters using expectation maximization and farthest first.
Myocardial Mass Contributes to the Discrepancy Between Anatomic Stenosis Severity Assessed by Intravascular Ultrasound and Fractional Flow Reserve in Intermediate Lesions of the Coronary Artery
Authors: Yoon Myeong‐Ho , Tahk Seung‐Jea , Lim Hong‐Seok , Yang Hyoung‐Mo , Seo Kyoung‐Woo , Choi Byoung‐Joo , Choi So‐Yeon , Hwang Gyo‐Seung , Park Jin‐Sun , Shin Joon‐Han 
Published: June 2017

ObjectivesThe present study investigated the major contributors to the discrepancy between the minimal lumen area (MLA) and fractional flow reserve (FFR).BackgroundThere was considerable discrepancy between MLA or diameter stenosis (DS) and FFR.MethodsWe enrolled 744 patients with intermediate stenoses of the left anterior descending artery (LAD). Summed epicardial coronary artery length distal to the target stenosis was obtained from each longest view of the vessels on the coronary angiograms. Mismatching was defined as a lesion with FFR of >0.80 and MLA smaller than the best cut‐off value (BCV) for predicting FFR of ≤0.80. Reverse mismatching was defined as a lesion with FFR of ≤0.80 and MLA larger than the BCV.ResultsSummed epicardial coronary artery length was longer at the lesions of proximal LAD than that of middle LAD (380 mm ± 82 mm vs. 341 mm ± 80 mm, P < 0.001). Reverse mismatching was found more frequently in the proximal than middle LAD (28.3% vs. 5.5%, P < 0.001). Independent predictors of FFR ≤ 0.80 were age, male, multi‐vessel disease, proximal LAD lesion, MLA, DS, plaque burden at distal reference, lesion length and summed epicardial coronary artery length. Proximal LAD lesion was an independent predictor of reverse mismatching (hazard ratio 3.162, 1.858–5.382, P < 0.001).ConclusionsMyocardial mass subtended by a lesion is an important factor predicting FFR ≤0.80 and discrepancy between FFR and MLA. Myocardial mass subtended by a lesion should be considered when determining the revascularization therapy by intravascular ultrasound parameters. © 2017 Wiley Periodicals, Inc.
Percutaneous obliteration of left ventricular cavity to eliminate aortic regurgitation and presumed coronary steal in an infant with hypoplastic left heart syndrome
Authors: El‐Said Howaida G. , Pockett Charissa R. , Moore John W. 
Published: June 2017

AbstractThe phenomenon of coronary steal is well known in the setting of HLHS (Hypoplastic Left Heart Syndrome) early after the Classical Norwood Operation. We report a rare case of an infant with HLHS [Severe Aortic Stenosis (AS), Mitral Stenosis (MS) and small Left Ventricle (LV)], who developed aortic regurgitation and presumed coronary steal late after the Sano Modification of the Norwood Procedure. Coronary steal developed secondary to progressive aortic and mitral regurgitation and resulted in poor right ventricular function and severe tricuspid regurgitation. We describe a novel interventional approach for obliteration of the LV cavity by using hydrogel coils. LV obliteration eliminated the presumed steal and resulted in improvement in ventricular function, tricuspid regurgitation and clinical course.
A case of bilateral hilar and mediastinal lymphadenopathy developing during treatment for Mycobacterium avium complex
Authors: Yamanaka Yumie , Sekine Akimasa , Yamakawa Hideaki , Baba Tomohisa , Okudela Koji , Takemura Tamiko , Ogura Takashi 
Published: June 2017

Key Clinical MessageWe report a rare case of an immunocompetent patient with Mycobacterium avium complex (MAC) disease in which bilateral hilar lymphadenopathy developed during anti‐MAC treatment. This case indicates that Propionibacterium acnes would be present and might be a cause of sarcoidosis even in patients with MAC.
Idiopathic left ventricular tachycardia following atrioventricular conduction block by rapid atrial tachycardia
Authors: Suenari Kazuyoshi , Mitsuba Naoya , Hirao Hidekazu , Ueda Hironori , Kihara Yasuki 
Published: June 2017

Key Clinical MessageThe present case demonstrated a rare situation alternating between a repetitive atrial tachycardia (AT) and ventricular tachycardia (VT). A unique induction mechanism was noted in which the VT was induced after Wenckebach AV node conduction block following the repetitive rapid AT.
Ion Pairing and Co‐facial Stacking Drive High‐Fidelity Bisulfate Assembly with Cyanostar Macrocyclic Hosts
Authors: Fatila Elisabeth M. , Twum Eric B. , Karty Jonathan A. , Flood Amar H. 
Published: June 2017

AbstractHydroxyanions pair up inside CH H‐bonding cyanostar macrocycles against Coulombic repulsions and solvation forces acting to separate them. The driving forces responsible for assembly of bisulfate (HSO4−) dimers are unclear. We investigated them using solvent quality to tune the contributing forces and we take advantage of characteristic NMR signatures to follow the species distributions. We show that apolar solvents enhance ion pairing to stabilize formation of a 2:2:2 complex composed of π‐stacked cyanostars encapsulating the [HSO4⋅⋅⋅HSO4]2− dimer and endcapped by tetrabutylammonium cations. Without cations engaged, a third macrocycle can be recruited with the aid of solvophobic forces in more polar solvents. The third macrocycle generates a more potent electropositive pocket in which to stabilize the anti‐electrostatic anion dimer as a 3:2 assembly. We also see unprecedented evidence for a water molecule bound to the complex in the acetonitrile solution. In methanol, OH H‐bonding leads to formation of 2:1 complexes by bisulfate solvation inside the macrocycles inhibiting anion dimers. Knowledge of the driving forces for stabilization (strong OH⋅⋅⋅O H‐bonding, CH H‐bonding, ion pairs, π‐stacking) competing with destabilization (Coulomb repulsion, solvation) allows high‐fidelity selection of the assemblies. Thermodynamic stabilization of hydroxyanion dimers also demonstrates the ability to use macrocycles to control ion speciation and stoichiometry of the overall assemblies.
Structure–Activity Relationship Studies on 6,7‐Dimethoxy‐2‐phenethyl‐1,2,3,4‐tetrahydroisoquinoline Derivatives as Multidrug Resistance Reversers
Authors: Teodori Elisabetta , Dei Silvia , Bartolucci Gianluca , Perrone Maria Grazia , Manetti Dina , Romanelli Maria Novella , Contino Marialessandra , Colabufo Nicola Antonio 
Published: June 2017

AbstractA series of derivatives were synthesized and studied with the aim to investigate the structure–activity relationships of the two P‐glycoprotein (P‐gp) modulators elacridar and tariquidar. Then, different aryl‐substituted amides were inserted, and to explore the effects of varying the amide function, the corresponding isosteric ester derivatives and some alkylamine analogues were synthesized. The new compounds were studied to evaluate their P‐gp interaction profile and selectivity toward the two other ABC transporters, multidrug‐resistance‐associated protein‐1 (MRP‐1) and breast cancer resistance protein (BCRP). Investigation of the chemical stability of the amide and ester derivatives toward spontaneous or enzymatic hydrolysis showed that these compounds were stable in phosphate‐buffered saline and human plasma. This study allowed us to evaluate the selectivity of the three series on the three efflux pumps and to propose the structural requirements that define the P‐gp interaction profile. We identified two P‐gp substrates, a P‐gp inhibitor, and three ester derivatives that were active on BCRP, which opens a new scenario in the development of ligands active toward this pump.
Discovery of Potent Dual Binding Site Acetylcholinesterase Inhibitors via Homo‐ and Heterodimerization of Coumarin‐Based Moieties
Authors: Pisani Leonardo , Catto Marco , De Palma Annalisa , Farina Roberta , Cellamare Saverio , Altomare Cosimo D. 
Published: June 2017

AbstractAcetylcholinesterase (AChE) inhibitors still comprise the majority of the marketed drugs for Alzheimer's disease (AD). The structural arrangement of the enzyme, which features a narrow gorge that separates the catalytic and peripheral anionic subsites (CAS and PAS, respectively), inspired the development of bivalent ligands that are able to bind and block the catalytic activity of the CAS as well as the role of the PAS in beta amyloid (Aβ) fibrillogenesis. With the aim of discovering novel AChE dual binders with improved drug‐likeness, homo‐ and heterodimers containing 2H‐chromen‐2‐one building blocks were developed. By exploring diverse linkages of neutral and protonatable amino moieties through aliphatic spacers of different length, a nanomolar bivalent AChE inhibitor was identified (3‐[2‐({4‐[(dimethylamino)methyl]‐2‐oxo‐2H‐chromen‐7‐yl}oxy)ethoxy]‐6,7‐dimethoxy‐2H‐chromen‐2‐one (6 d), IC50=59 nm) from originally weakly active fragments. To assess the potential against AD, the disease‐related biological properties of 6 d were investigated. It performed mixed‐type AChE enzyme kinetics (inhibition constant Ki=68 nm) and inhibited Aβ self‐aggregation. Moreover, it displayed an outstanding ability to protect SH‐SY5Y cells from Aβ1‐42 damage.
Surface‐Modified Gold Nanoparticles Possessing Two‐Channel Responsive EuIII/TbIII Cyclen Complexes as Luminescent Logic Gate Mimics
Authors: Truman Laura K. , Bradberry Samuel J. , Comby Steve , Kotova Oxana , Gunnlaugsson Thorfinnur 
Published: June 2017

AbstractThe development of material‐supported molecular logic gate mimics (MGLMs) for contained application and device fabrication has become of increasing interest. Herein, we present the formation of ≈5 nm gold nanoparticles (AuNPs) that have been surface‐modified (via a thiol linkage) with heptadentate cyclen‐based complexes of europium and terbium for sensing applications using delayed lanthanide luminescence and as integrated logic gate mimics within competitive media.