bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sudden Cardiac Death Initiative: A National Campaign to Raise Awareness of Sudden Cardiac Death
Authors: Sobhy Mohamed , Khamis Hazem , Mehanna Radwa , Elshal Ahmed , Ghanem Noha 
Published: September 2024

Abstract

Introduction
Moderate aerobic exercise is widely recognized for lowering the risk of coronary heart disease and mortality. However, for individuals with certain heart conditions, exercise can paradoxically increase the risk of sudden cardiac death (SCD), the leading cause of sudden death among athletes. Despite its rarity, with an estimated occurrence of 1 in every 40,000 to 80,000 athletes annually, SCD has a profound emotional and social impact on communities.

Aim
The Sudden Cardiac Death Initiative aims to reduce the incidence of SCD in Egypt by enhancing community health education and raising awareness among the public and athletes about the risks and preventive measures associated with SCD. The initiative also seeks to provide essential first aid and CPR training and equip sports facilities with Automated External Defibrillators (AEDs).

Methods
The initiative is a collaboration between the Cardiovascular Research Education and Prevention (CVREP) Foundation, Wadi El Neel Hospital, the Egyptian Ministry of Health and Population, the Ministry of Youth and Sports, and Expand (one of ICOM Group). It was launched on June 14, 2024, during the CardioAlex 2024 Conference at Bibliotheca Alexandrina. The event featured educational videos, presentations on SCD cases, and discussions on cardiovascular disease risks and prevention strategies led by Dr. Mohamed Sobhy and Dr. Hazem Khamis.
Key activities included:Awareness sessions with over 300 attendees, assessed through pre- and post-session evaluations.First aid and CPR training programs.Supplying sports facilities with AEDs and other critical medical equipment.

Results
Initial outcomes indicated a significant increase in awareness and engagement from sports club presidents and representatives. The initiative highlighted the importance of addressing SCD through community education and improved medical alertness in sports facilities.

Conclusion
The Sudden Cardiac Death Initiative is a crucial step toward reducing SCD risks in Egypt. By focusing on awareness, education, and equipping sports facilities with advanced medical equipment, the initiative aims to save lives and enhance the safety and well-being of athletes and the general population. Continued collaboration among the Ministry of Health and Population, Ministry of Youth and Sports, CVREP, Wadi El Neel Hospital, and Expand is essential to the sustained impact of this initiative.
V-pipe 3.0: a sustainable pipeline for within-sample viral genetic diversity estimation
Authors: Fuhrmann Lara , Jablonski Kim Philipp , Topolsky Ivan , Batavia Aashil A , Borgsmüller Nico , Baykal Pelin Icer , Carrara Matteo , Chen Chaoran , Dondi Arthur , Dragan Monica , Dreifuss David , John Anika , Langer Benjamin , Okoniewski Michal , du Plessis Louis , Schmitt Uwe , Singer Franziska , Stadler Tanja , Beerenwinkel Niko 
Published: September 2024

Abstract
The large amount and diversity of viral genomic datasets generated by next-generation sequencing technologies poses a set of challenges for computational data analysis workflows, including rigorous quality control, scaling to large sample sizes, and tailored steps for specific applications. Here, we present V-pipe 3.0, a computational pipeline designed for analyzing next-generation sequencing data of short viral genomes. It is developed to enable reproducible, scalable, adaptable, and transparent inference of genetic diversity of viral samples. By presenting 2 large-scale data analysis projects, we demonstrate the effectiveness of V-pipe 3.0 in supporting sustainable viral genomic data science.
Altered Lignin Accumulation in Sorghum Mutated in Silicon Uptake Transporter SbLsi1
Authors: Rivai Reza Ramdan , Yamazaki Kiyoshi , Kobayashi Masaru , Tobimatsu Yuki , Tokunaga Tsuyoshi , Fujiwara Toru , Umezawa Toshiaki 
Published: September 2024

Abstract
Sorghum [Sorghum bicolor (L.) Moench] has been receiving attention as a feedstock for lignocellulose biomass energy. During the combustion process, ash-containing silicon (Si) can be produced, which causes problems in furnace maintenance. Hence, lowering Si content in plants is crucial. However, limiting Si supply to crops is difficult in practice because Si is abundant in the soil. Previously, an Si uptake transporter (SbLsi1) has been identified, and an Si-depleted mutant has also been generated in the model sorghum variety BTx623. In this study, we aimed to investigate the changes induced by a mutation in SbLsi1 on the accumulation and structure of lignin in cell walls. Through chemical and NMR analyses, we demonstrated that the lsi1 mutation resulted in a significant increase in lignin accumulation levels as well as a significant reduction in Si content. At least some of the modification was induced by transcriptional changes, as suggested by the upregulation of phenylpropanoid biosynthesis-related genes in the mutant plants. These findings derived from the model variety could be useful for the future development of practical cultivars with high biomass and less Si content for bioenergy applications.
Naringenin chalcone carbon double-bond reductases mediate dihydrochalcone biosynthesis in apple leaves
Authors: Yauk Yar-Khing , Dare Andrew P , Cooney Janine M , Wang Yule , Hamiaux Cyril , McGhie Tony K , Wang Mindy Y , Li Pengmin , Atkinson Ross G 
Published: September 2024

Abstract
Dihydrochalcones (DHCs) are flavonoids produced as a side branch of the phenylpropanoid pathway. DHCs are found at high concentrations in apples (Malus spp.) but not in pears (Pyrus spp.) or other members of the Rosaceae. Biosynthesis of DHCs in apple has been hypothesized to occur via reduction of p-coumaroyl CoA by a Malus × domestica hydroxycinnamoyl CoA double-bond reductase (MdHCDBR) followed by the action chalcone synthase to produce phloretin or via direct reduction of naringenin chalcone to phloretin via an unknown enzyme. In this study, we report that genetic downregulation of MdHCDBR does not reduce DHC concentrations in apple leaves. We used comparative transcriptome analysis to identify candidate naringenin chalcone reductases (NCRs), designated MdNCR1a–c, expressed in apple leaves but not fruit. These MdNCR1 genes form an expanded gene cluster found exclusively in apple. Transient expression of MdNCR1 genes in Nicotiana benthamiana leaves indicated they produced DHCs at high concentrations in planta. Recombinant MdNCR1 utilized naringenin chalcone to produce phloretin at high efficiency. Downregulation of NCR genes in transgenic apple reduced foliar DHC levels by 85% to 95%. Reducing DHC production redirected flux to the production of flavonol glycosides. In situ localization indicated that NCR proteins were likely found in the vacuolar membrane. Active site analysis of AlphaFold models indicated that MdNCR1a–c share identical substrate binding pockets, but the pockets differ substantially in related weakly active/inactive NCR proteins. Identifying the missing enzyme required for DHC production provides opportunities to manipulate DHC content in apple and other fruits and has other applications, e.g. in biofermentation and biopharming.
Comparative effect of oral drugs in improving spasticity of different etiology: a network meta-analysis
Authors: Otero-Luis Iris , Martínez-Rodrigo Arturo , Cavero-Redondo Iván , Moreno-Herráiz Nerea , López-López Samuel , Saz-Lara Alicia 
Published: September 2024

Abstract

Introduction
Spasticity, a prevalent manifestation of various neurological conditions, significantly impacts the quality of life of patients. Research on the effects of oral drugs on spasticity has produced controversial results. Thus, the aim of this network meta-analysis was to compare the efficacy of oral drugs for improving spasticity in patients with different etiologies.

Methods
We searched four different databases from their inception to 30 November 2023. A network meta-analysis using a frequentist perspective was conducted to assess the effects of different oral drugs on spasticity, evaluated by the modified Ashworth scale.

Results
Our findings showed that, in a frequentist network meta-analysis, eperisone, diazepam, and baclofen had significantly greater spasticity, as measured by the modified Ashworth scale, than did the placebo (MD: −0.80; 95% CIs: −1.42, −0.18; MD: −0.68; 95% CIs: −1.28, −0.09; MD: −0.58; 95% CIs: −1.11, −0.06, respectively).

Conclusion
In summary, our study confirmed that eperisone, diazepam, and baclofen could be effective approaches for reducing spasticity of different etiologies and could be useful approaches for improving patient quality of life.
Key messages What is already known on this topic: The impact of oral drugs, such as baclofen, gabapentin, tizanidine, and dantrolene, in the treatment of spasticity has been documented. What this study adds: This study determines which of the oral drugs aimed at treating spasticity is the most effective across different etiologies. How this study might affect research, practice, or policy: This study suggests tailored treatment strategies for spasticity based on its etiology.
Prospective Trial of Sacroiliac Joint Fusion Using 3D-Printed Triangular Titanium Implants
Authors: Patel Vikas , Meyer S. Craig , Kovalsky Don , Lockstadt Harry , Farris Jim , Limoni Robert , Chowdhary Abhineet , Yuan Philip , Langel Casey , Kranenburg Andy , Tender Gabriel 
Published: September 2024

Study Design.
Prospective, multicenter, single arm study with 5-year follow up

Objective.
To report long-term (5-year) safety, effectiveness, and radiographic outcomes after sacroiliac (SI) joint fusion (SIJF) with 3D-printed triangular titanium implants (TTI) and compare them with solid titanium plasma spray coated implants

Summary of Background Data.
A large literature base supports minimally invasive SIJF with TTI for chronic SIJ dysfunction.

Methods.
SIJF with TTI was performed in 51 subjects and scheduled follow-up occurred at 3, 6, 12, 24 and 60 months. Postoperative visits included quality of life questionnaires and functional tests. A high-resolution CT scan was performed at either 6 or 12 months, and again at 60 months. All CT scans were interpreted by an independent musculoskeletal radiologist.

Results.
5-year follow-up was available in 36 of 51 subjects (71%). At 5 years, improvements in SI joint pain (58.1 points [0-100 scale]), SIJ-related disability (Oswestry Disability Index [ODI], 25.4 points) and quality of life (EuroQOL 5D, 0.24 points, and EuroQOL visual analog scale, 13.4 points) were sustained and showed no signs of diminution. The percentage of patients using opioids for SIJ pain decreased markedly from baseline (57%) to 5 years (17%). Physical function tests showed persistent improvements from baseline. There was no evidence of device breakage, migration or subsidence and few late adverse events occurred attributable to the device. Independent analysis of CT scans showed bone opposed to implants in all cases, and evidence of bone bridging in 90% of cases, and no evidence of radiolucency or other adverse bone reactions.

Conclusions.
Prospective 5-year follow-up showed that SIJ fusion using 3D-printed TTI resulted in immediate, marked, and persistent improvements in pain and quality of life, with improved physical function, reduced opioid use, and a low rate of revision surgery. Radiographic evidence showed implant integration and joint fusion.

Level of evidence.
Level II.
Cosmetic implant placement in the female breast yields an altered local and systemic immune response: evidence for breast cancer immunosurveillance
Authors: Timmerman Ramsey , Allison Sophia , Dolivo David , Jagasia Puja , Gargiulo Kristine , Battu Shreya , Hong Seok Jong , Helenowski Irene , Kim John YS , Fracol Megan 
Published: September 2024

Background:
Women with cosmetic implants have lower rates of future breast cancer than the general population. We hypothesized the implant foreign body response could induce a local protective anti-cancer immunosurveillance. We expanded on our previous finding which showed women with breast implants have elevated antibody responses to certain breast cancer proteins.

Methods:
Blood samples and breast tissue were collected from women undergoing first time breast augmentation (implant-naive, IN) and revision breast augmentation (implant-exposed, IE). Sera were collected and antibody levels to common breast cancer proteins were quantified by enzyme-linked immunosorbent assay (ELISA). RT-PCR was performed on breast tissue samples to quantify immune-related gene expression levels between IN and IE. Bulk RNA sequencing was performed to identify differentially expressed genes and altered signaling pathways in the breasts of IN versus IE.

Results:
In total, 188 patients were recruited (117 IN, 71 IE). Data demonstrated that IE patients had higher levels of antibodies to MUC-1, ER, and mammaglobin A compared to IN patients. MUC-1 expression was found to be higher in IE compared to IN breast tissue. RNA-seq analysis demonstrated upregulated pathways in IE breast tissue for B cell activation and development, Th2 related genes, T cell activation, chemotactic factors, and responses to estrogen.

Conclusion:
This is the first study to demonstrate that peri-implant inflammation extends beyond the implant capsule to breast parenchyma. Women with breast implants have more activated B cells in the breast parenchyma and elevated antibody responses to breast cancer antigen.
Targeting Kidney Inflammation After Brain Death and Cold Storage: Investigating the Potential of an NLRP3 Inflammasome Inhibitor (MCC950) for Preconditioning Donor Kidneys
Authors: Gonçalves Naiane do Nascimento , Caldas Heloisa Cristina , Marzochi Ludimila Leite , Baptista Maria Alice Sperto Ferreira , Correia Cristiano de Jesus , Faloppa Ana Cristina Breithaupt , Moreira Luiz Felipe Pinho , Abbud-Filho Mario 
Published: September 2024

Background.
Brain death (BD) and cold storage (CS) are critical factors that induce inflammation in donor kidneys, compromising organ quality. We investigated whether treating kidneys from BD rats with an inflammasome Nod-like receptor family pyrin domain containing 3 (NLRP3) inhibitor (MCC950) followed by CS could reduce kidney inflammation.

Methods.
BD rats were assigned to MCC950-treated or nontreated (NT) groups. Kidneys were evaluated immediately before CS (T0) and after 12 h (T12) and 24 h (T24) of CS. Mean arterial pressure, serum creatinine, gene/protein expression, and histology were evaluated.

Results.
At T0, MCC950 treatment did not affect mean arterial pressure but tended to reduce serum creatinine and ameliorated the histological score of acute tubular necrosis. However, MCC950 reduced NLRP3, caspase-1, interleukin (IL)-1β, IL-6, Kim-1, nuclear factor kappa B, tumor necrosis factor alpha, and caspase-3 gene expression while increasing IL-10 cytokine gene expression. After 12 h of CS, only the expression of the NLRP3 and caspase-1 genes decreased, and after 24 h of CS, no further changes in the gene expression profile were observed. The levels of the inflammasome proteins NLRP3, caspase-1, and IL-1β consistently decreased across all time points (T0, T12, and T24).

Conclusions.
These findings suggest that MCC950 treatment holds promise for mitigating the proinflammatory state observed in kidneys after BD and CS.
Prognostic value of systemic immune-inflammation index in patients with locally advanced renal cell carcinoma
Authors: Lin Tsu-Chen , Su Shih-Huan , Huang Wen-Kuan , Chen Dong-Yi , Pang See-Tong , Chuang Cheng-Keng 
Published: September 2024

Purpose:
To assess the prognostic value of systemic immune-inflammation index (SII) for disease progression in locally advanced renal cell carcinoma (RCC).

Materials and methods:
A total of 97 patients with locally advanced RCC who underwent curative surgery between January 1, 2010 and December 31, 2018 were included in this retrospective study. The study was approved by the Institutional Review Board of Linkou Chang Gung Memorial Hospital with an IRB number of 202000092B0. Outcomes analyzed were the disease-free survival (DFS) of patients with locally advanced RCC and the prognostic value of SII.

Results:
Among 97 locally advanced RCC patients, disease progression was noted in 48.5% of the patients. The median DFS was 55.6 months (95% confidence interval: 45.1–66.2 months). An elevated SII greater or equal to 514 was associated with a poorer DFS. After multivariate analyses of possible predictive factors, SII remained statistically associated with a poorer DFS.

Conclusion:
An elevated SII was associated with a poorer DFS in patients with locally advanced RCC. SII could be used as a prognostic factor to guide the use of adjuvant therapy after curative surgery for patients with locally advanced RCC.
Roles of tranexamic acid on bleeding in percutaneous nephrolithotomy procedure: A systematic review and meta-analysis
Authors: Samudera I Made Ari , Hamid Agus Rizal Ardy Hariandy , Nandana Pandu Ishaq 
Published: September 2024

Purpose:
Bleeding is the most common percutaneous nephrolithotomy (PCNL) complication. We investigated the role of tranexamic acid (TXA), which has numerous benefits in trauma and elective surgery, in PCNL.

Materials and methods:
Systematic reviews were performed using PubMed, Science Direct, Scopus, and Google Scholar. This study included randomized clinical trials that compared the effects of TXA and placebo during PCNL. The publication date, which was a limitation of the study, was disregarded. The analysis only included studies published in English that were available in full text. Patients with kidney stones who underwent PCNL were also enrolled. The outcomes comprised blood loss, hemoglobin decrease, blood transfusion rate, and length of hospital stay.

Results:
A total of 1377 patients from 8 studies were included. TXA was correlated with a low rate of blood transfusion (odds ratio = 0.35 [0.22–0.56]; P < 0.00001), blood loss (mean difference [MD] = −66.88 mL [−101.54 to −32.23]; P = 0.0002), decreased hemoglobin levels (MD = −0.67 g/dL [−0.96 to −0.38]; P < 0.00001), and reduced length of hospital stay (MD = −0.61 day [−1.04 to −0.18]; P = 0.005).

Conclusion:
The promising roles of TXA in PCNL procedures have been revealed. Further studies involving additional clinical trials, particularly those that assess interactions and adaptations to patients’ comorbidities, are suggested.
Key roles of the zona pellucida and perivitelline space in promoting gamete fusion and fast block to polyspermy inferred from the choreography of spermatozoa in mice oocytes
Authors: Dubois Yaëlle , Favier Sophie , Martin-Fornier Nathan , Omrane Mohyeddine , Stroebel David , Perez Eric , Barbaux Sandrine , Ziyyat Ahmed , Rodriguez Nicolas , Gourier Christine 
Published: September 2024

AbstractMammalian fertilization is still lacking a comprehensive understanding of gamete fusion and block to polyspermy mechanisms. One reason is that they are highly dynamic processes involving transient events that can only be revealed and characterized by direct observation. To extend while challenging existing knowledge, this study applies real time brightfield and confocal imaging to inseminated ZP-intact mouse oocytes and statistical analyses to establish an accurate dynamic picture of the cascade of events leading to fusion and prevention of polyspermy in conditions as close to physiology as possible. These observations allow to characterize the roles of the different components of the oocyte (i.e. zona pellucida (ZP), perivitelline space (PVS), oocyte plasma membrane (OPM)) and the spermatozoon (i.e. head, flagellum) in promoting fertilization and preventing polyspermy. The kinetics we have determined challenge dogmas by showing that: (i) the first sperm is not necessarily the one that fertilizes in mice pointing to the existence of other post-penetration fertilization factors, (ii) the ZP block resulting from the cortical reaction is too slow to contribute to the prevention of polyspermy in mice. On the other hand, it evidences that the ZP directly contributes to polyspermy block in two other ways: (a) as a naturally effective entry barrier for the spermatozoon (independent of any block caused by fertilization), (b) as an effective exit barrier for components released by the OPM, which may contribute to a fast PVS block to polyspermy through the neutralizing of unwanted spermatozoa in the PVS. Moreover, our observations reveal that the ZP plays a key role in fertilization itself by channeling the flagellar oscillations of spermatozoa in the PVS to make them conducive to fusion.
Osteocyte-derived erythroferrone regulates liver hepcidin during stress erythropoiesis
Authors: Myneni Vamsee D , Parashar Abhinav , Szalayova Ildikó , Mezey Eva 
Published: September 2024

AbstractOur knowledge of which bone marrow cells affect red cell production is still incomplete. To explore the role of osteocytes in the process we performed bulk RNAseq of osteocytes isolated from control and phlebotomized mice. The top-upregulated gene following phlebotomy wasFam132b, erythroferrone (Erfe).Erfeexpression in osteocytes was also upregulated after erythropoietin (EPO) treatment and hypoxiain vitro. To explore if osteocytes contribute to the systemic ERFE levels, we generated two mouse models. We first transplanted wild-type BM inErfe-/-mice creating a model where ERFE is produced in the BM but not by osteocytes. After phlebotomy, liver hepcidin suppression was significantly lower in mice where the osteocytes could not produce ERFE. To confirm that osteocytes are responsible for this difference, we generated mice lacking EPO receptors in osteocytes by crossingEporflox/floxandDmp1-Cre mice. After phlebotomy, these mice showed reduced hepcidin suppression in the liver and higher circulating serum hepcidin levels compared to controls. Our work identified a novel function of osteocytes in suppressing systemic hepcidin levels during stress erythropoiesis.
GERMIN3 plays a role in plasmodesmatal gating to regulate meristem activation related to tuberisation, tuber dormancy release and stem branching in potato
Authors: Campbell Raymond , Cowan Graham , Wurzinger Bernhard , Ducreux Laurence J.M. , Dessoly Jimmy , Guo Wenbin , Zhang Runxuan , Morris Jenny A. , Hedley Pete , Wahl Vanessa , Taylor Mark A. , Hancock Robert D. 
Published: September 2024

SummaryGERMIN3has previously been identified as a target of the tuberigen activation complex suggesting a function in potato tuberisation but its role is presently unknown.To understand the role of GERMIN3 we analysed morphological, agronomic and molecular phenotypes in transgenic lines.GERMIN3over-expressing lines ofSolanum tuberosumssp.andigenaexhibited increased tuber yields under permissive conditions and enhanced tuber numbers. Post-harvest tuber sprouting exhibited reduced apical dominance with increased numbers of sprouts. Apical dominance was reduced in aerial tissues of mature plants where stem growth from axillary buds was activated. Similar results were observed in the commercial cultivar Desiree. Over-expression ofGERMIN3had no impact on the expression ofSP6A, a positive regulator of tuberisation orTFL1B, a negative regulator. The GERMIN3 protein localised to the endoplasmic reticulum and transient expression inN. benthamianaleaves resulted in plasmodesmatal gating allowing intercellular transport of GFP-tagged sporamin independent of GERMIN3 oxalate oxidase activity.GERMIN3 affects tuberisation and other developmental processes by facilitating meristem activation. This identifies GERMIN3 as a novel protein associated with control of plasmodesmatal transport and supports the importance of plasmodesmatal gating in the regulation of key potato developmental processes.