bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Simple and Fast Optical Clearing Method for Whole‐Mount Fluorescence In Situ Hybridization (FISH) Imaging
Authors: Suh Junwoo , Liu Yehe , Smith Jordan , Watanabe Michiko , Rollins Andrew M. , Jenkins Michael W. 
Published: October 2024

ABSTRACTWe report a single‐step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. We previously demonstrated microscopy imaging with immunohistochemistry and genetic reporters using a technique called lipid‐preserving refractive index matching for prolonged imaging depth (LIMPID). Our protocol reliably produces high‐resolution three‐dimensional (3D) images with minimal aberrations using high magnification objectives, captures large field‐of‐view images of whole‐mount tissues, and supports co‐labeling with antibody and FISH probes. We also custom‐designed FISH probes for quail embryos, demonstrating the ease of fabricating probes for use with less common animal models. Furthermore, we show high‐quality 3D images using a conventional fluorescence microscope, without using more advanced depth sectioning instruments such as confocal or light‐sheet microscopy. For broader adoption, we simplified and optimized 3D‐LIMPID‐FISH to minimize the barrier to entry, and we provide a detailed protocol to aid users with navigating the thick and thin of 3D microscopy.
Composite adsorbent of Terminalia catappa fruit shell‐derived activated carbon and chitosan for enhanced removal of chromium(VI): kinetics, thermodynamics, equilibrium and adsorption mechanism
Authors: Nguyen My Linh , Nguyen Thanh Tung , Nguyen Thi Tuyet Trinh , Chau Nha Truc T. , Ngo Hoang Long , Tran Anh T.K. , Juang Ruey‐Shin 
Published: October 2024

AbstractBACKGROUNDChromium, which is widely employed in many industries, has a high toxicity. In this research, activated carbon derived from Terminalia catappa fruit shells was combined with chitosan (TCAC‐CS) to enhance adsorption capability of hexavalent chromium [Cr(VI)] in wastewater.RESULTSDifferent Cr(VI) adsorption parameters were investigated. Two isotherm and two kinetic models were employed to understand the adsorption mechanism. The thermodynamic characteristics of Cr(VI) adsorption, namely the Gibbs free energy change (ΔG), enthalpy change (ΔH) and entropy change (ΔS), also were computed. The best adsorption capability was achieved at pH 3, adsorbent dose 2.5 g L−1, temperature 35 °C and initial Cr(VI) concentration 50 mg L−1. The Cr(VI) adsorption capacity reached 184.45 mg g−1, as fitted by the Langmuir isotherm, which is superior when compared to most biomass‐based carbonaceous adsorbents. The negative ΔG value shows the spontaneous nature of the Cr(VI) adsorption process on the TCAS‐CS. Additionally, Fourier transform infrared characterization confirmed that the incorporation of chitosan to activated carbon resulted in chemical adsorption of Cr(VI) via chelating instead of sole physical adsorption via hydrogen bonding, which greatly improved the adsorption ability of the materials.CONCLUSIONTCAC‐CS demonstrated potential utilization as an efficient, low‐cost and environmentally friendly adsorbent in the removal of Cr(VI) from wastewater. © 2024 Society of Chemical Industry (SCI).
Lewis Base Promoted [4+2] Annulation of o‐Acylamino‐Aryl Morita‐Baylis‐Hillman Carbonates With Isatylidene Malononitriles: Facile Access to Spiro[Indolin‐3,2′‐Quinoline] Frameworks
Authors: Shen Li‐Wen , Luo Mao‐Dan , Zhou Wen‐Yi , Luo Yuan‐Ying , Xiang Min , Jia Yun‐Qing , Yuan Shu‐Pei , Wang Guang‐Wei 
Published: October 2024

ABSTRACTA novel [4+2] annulation reaction between o‐acylamino‐aryl MBH carbonates and isatylidene malononitriles has been successfully developed. This methodology exhibits excellent substrate tolerance, affording a series of spiro[indolin‐3,2′‐quinoline] framework compounds with yields up to 98%. The scalability of the reaction is also noteworthy, as evidenced by the 95% yield of the final product upon a 50‐fold scale‐up.
Maltobionic acid protects against ovariectomy‐induced osteoporosis by suppressing bone resorption
Authors: Nishio Ayaka , Suehiro Daiki , Nakamura Honami , Moriwaki Yuichiro , Nakagawa Hiroshi , Ukai Yoko , Watanabe Akio , Yonezawa Takayuki , Woo Je Tae , Fukami Ken , Ohnishi Motoko 
Published: October 2024

AbstractBackgroundOsteoporosis is a debilitating disease characterized by decreased bone density. In this study, we evaluated the anti‐osteoporotic effect of maltobionic acid (MB), one of the components of honey, and calcium maltobionate (MBCa) on bone density and metabolism using a mouse model of osteoporosis. The underlying mechanisms of MB and MBCa action were also investigated.ResultsOvariectomized (OVX) mice were fed diet containing MB or MBCa for 80 days, and femoral bone mineral content and mineral density (BMD) were evaluated. As expected, OVX reduced BMD; however, the administration of MB and MBCa significantly prevented this decrease. Furthermore, MB and MBCa treatment significantly reduced the serum levels of tartrate‐resistant acid phosphatase 5b (TRACP‐5b), a bone resorption marker, and significantly increased the levels of serum calcitonin, compared to those in the OVX control group. In vitro, the relatively high‐dose levels of MB and MBCa inhibited osteoclast differentiation by decreasing the protein expression of nuclear factor of activated T‐cell cytoplasmic 1 (NFATc1), the master transcription factor of osteoclastogenesis.ConclusionOur results showed that osteoporotic mice treated with MB and MBCa had improved bone density and bone metabolism compared to the OVX controls. Moreover, it was hypothesized herein that the suppressive effect on osteoclast differentiation slightly contributed to these results concomitantly. These findings suggest that the intake of MB or MBCa may contribute to the maintenance of bone health, including the prevention of primary osteoporosis.
Effect of green tea or black tea extract on lipid and protein oxidation in Cantonese sausage
Authors: Xu Weibing , Nie Chunlin , Li Zhicheng , Qiu Shuxian , Xiang Junyi , Zhou Yongqiang , Tomasevic Igor , Sun Weizheng 
Published: October 2024

AbstractBACKGROUNDNatural polyphenols offer a safer alternative to synthetic antioxidants in meat products. This study investigated the efficacy of green tea and black tea extracts as natural antioxidants in Cantonese sausages to inhibit lipid and protein oxidation.RESULTSSausages were prepared with the addition of different concentrations – 100, 300, and 600 mg kg−1 total polyphenols (TP) – of green tea or black tea extract. Oxidation of the sausages was assessed through thiobarbituric acid reactants, carbonyl content, and thiol content, whereas consumer acceptability was evaluated based on texture, color, and sensory analysis. The tea extracts inhibited malondialdehyde production and reduced the thiobarbituric acid reactive substance value from 23.72 mmol MDA g−1 to less than 1.94 mmol MDA g−1. However, the addition of tea extracts decreased the thiol content and caused the loss of myosin heavy chain and actin bonds in sodium dodecyl sulfate polyacrylamide gel electrophoresis. Although the addition of tea extracts increased the redness and hardness of the sausage, no significant difference in consumer acceptance between the control and treatment groups was observed in the sensory analysis.CONCLUSIONThe tea extract inhibited the oxidation of lipids in Cantonese sausage. There was no negative effect on the sensory characteristics of sausages. The use of tea extracts as natural antioxidants in Cantonese sausage is therefore feasible. © 2024 Society of Chemical Industry.
More Than Taste: Effects of Visitor's Cognitive Appraisal on the Complete Satisfaction of Wine Tourism
Authors: Wang Xi , Zheng Jie , Luo Yi 
Published: October 2024

ABSTRACTWine tourism is gaining popularity as visitors seek unique experiences. This study investigated the effects of tangible and intangible factors on visitors' satisfaction with wine tourism. Free cancellation, multi‐language support, tour guides, service animals, tasting fees, and refreshments positively affected satisfaction, while COVID‐19 measures, larger groups, and more tastings had negative impacts. Positive emotions in reviews correlated with higher satisfaction, while negative emotions lowered it. The research contributes to understanding wine tourism and provides insights for operators to improve satisfaction and reform the industry post‐pandemic.
Exploring the Impact of Biochar‐Dolomite Application at Different Pyrolysis Temperatures on Acid Sulfate Soil Properties
Authors: Sulaiman Syazwan , Navaranjan Namasivayam , Hernandez‐Ramirez Guillermo , Sulaiman Zohrah , Liew Kathereen 
Published: October 2024

ABSTRACTThe use of biochar as a soil amendment for acid soil, particularly acid sulfate soil, has garnered significant interest due to its capacity to improve soil properties, which is further accentuated when combined with dolomite. However, the extent to which the magnitude and direction of this effect vary with biochar produced at different pyrolysis temperatures remains uncertain. We conducted an incubation by mixing soil with dolomite and biochar types derived from Melastoma malabathricum (MMBC) and Dicranopteris linearis (DLBC) at three temperatures (300, 500, and 700°C). The incorporation of dolomite with both biochar types led to significant improvements in soil pH, available P, available Fe, exchangeable K, and exchangeable Ca compared to soil amended with dolomite only. These enhancements were likely attributed to the release of alkalinity, which further raised soil pH, as well as the direct dissolution of P, K, and Ca from the biochar. Moreover, the reduced Fe availability was likely due to the complexation of Fe onto surface functional groups of biochar. Furthermore, these improvements surpassed the effect of the initial feedstock and became increasingly pronounced with higher pyrolysis temperatures. However, an antagonistic effect of dolomite and biochar co‐incorporation was observed, particularly in the relative reduction of soil exchangeable Mg with both biochar types, and a decrease in the soil available N with MMBC produced at 300 and 500°C compared to dolomite addition alone, which could be due to the adsorption of Mg and N onto biochar surface. The results indicate that concurrent application of dolomite and biochar can change the acid sulfate soil properties to varying magnitudes and in different directions, largely regulated by the characteristics of biochar as well as the probable interplay with dolomite. Consequently, selecting the appropriate biochar that aligns with the desired soil properties is crucial in managing dolomite‐treated acid sulfate soil.
Erosion and Deposition Patterns Over a Wind‐Blown Sand Dune Behind a High Vertical‐Type Sand Fence
Authors: Dong Hongguang , Cheng Jianjun , Li Yupeng , Chen Yuanqing , Zhang Ruoyuan , Chen Donghe 
Published: October 2024

ABSTRACTSand dunes are the primary factor influencing wind‐sand disasters. To explore the evolution law of wind‐blown sand dunes surrounding high vertical‐type sand fences along desert railways, numerical simulation analyses based on computational fluid dynamics coupled with a discreet element method of the wind‐sand flow field and sand particles trajectories around sand fences were conducted. The focus of this study was the Yandun section of the Lanzhou‐Xinjiang Railway in Xinjiang, China (42.38°N, 93.95°E). By combining ERA5 wind data with field measurements and monitoring, a three‐dimensional coupling model was established. According to the results of L9 (33) orthogonal tests, the sensitivity of the three factors to the evolution of sand dunes can be ordered as wind speed > porosity > height, indicating that wind speed is closely related to sand dune evolution (p < 0.05). The results of single‐factor tests indicate that the structure of wind‐sand flow fields, sand accumulation patterns, and sand particle movement trajectories vary significantly under different wind speeds. The sand accumulation rates on the windward slope of the sand dune are 29.67%, 25.91%, and 20.04%, while on the leeward slope, these rates are 40.33%, 34.09%, and 29.96%, respectively. The obtained information on the evolution law of sand dunes surrounding high vertical‐type sand fences provides a scientific basis for wind‐sand prevention measures along railways in desert regions.
Impacts of counting protocols for light microscopy on estimates of biodiversity and algal density of phytoplankton
Authors: Jacobs Pascalle , Serre‐Fredj Léon , Koeman Reinoud P. T. , van den Oever Anneke , Peck Myron A. , Philippart Catharina J. M. 
Published: October 2024

AbstractKnowledge on the biodiversity and abundance of phytoplankton is key for many ecological and societal (e.g., blue growth) questions. Gathering temporal variation and spatial patterns on key indicators requires reliable and standardized protocols on sampling, species identification and counting. Numerous methods are used but consequences for comparing the biodiversity and abundance of phytoplankton of these different techniques are not well known. We evaluated the consequences of different counting protocols using light microscopy (i.e., subsampling transects or wedges within counting chambers) for these indices using samples collected weekly to bi‐weekly (n = 398, 2009–2018) from the Wadden Sea (southern North Sea). Phytoplankton cells were counted (by one person under similar conditions) in a fixed number of viewing fields (58, 70, and 29) at three respective magnifications (10 × 100, 10 × 40, and 10 × 10). Patterns in the spatial distribution of phytoplankton cells varied among species and clustering of cells occurred in more than one‐fifth of the samples. This will induce error in the conversion from counts (per viewing field) to abundance (cells mL−1). Our present effort resulted in a high accuracy (95%) in overall cell abundances. This was not the case for species richness, for example, capturing 90% of all species present in the sample would require an almost threefold increase in effort for the 10 × 40 and 10 × 10 magnifications. We recommend that counting methods be tailored to the main research objectives and that counting protocols should quantify uncertainty as well as potential bias to provide an estimation of the error in phytoplankton abundance and species composition.
Putting an artificial intelligence‐generated label on it comes naturally
Authors: Sigurdsson Valdimar , Larsen Nils Magne , Folwarczny Michał , Dubois Magalie , Fagerstrøm Asle 
Published: October 2024

AbstractClimate change and the advent of artificial intelligence‐generated content are reshaping wine marketing. The interplay between consumer focus on naturalness and sustainable farming practices and the proliferation of artificial intelligence‐generated content represents a particularly salient area of research. However, the extent to which the presence of fictitious artificial intelligence‐generated labels and backgrounds impacts consumers' willingness to buy and pay for wine has yet to be addressed. This research contributes to the growing body of literature on consumer susceptibility to sustainability signaling and artificial intelligence greenwashing, focusing on the impact of backgrounds and labels with different degrees of perceived naturalness. Three experiments demonstrate that wines bearing artificial intelligence‐generated sustainability labels and third‐party accredited sustainability labels reliably exhibit an increased willingness to buy and pay compared to those without sustainability labels. These findings indicate that fictitious, artificial intelligence‐generated, and accredited labels are equally effective in influencing consumer wine choices. Customer susceptibility to food labels and wine knowledge and involvement also significantly predict willingness to buy across studies, validating the Customer Susceptibility to Front‐of‐Package Food Labeling scale. These findings highlight the necessity for future studies to investigate the role of responsible labeling, the susceptibility of customers to such labels, and the potential hazards associated with greenwashing practices involving artificial intelligence‐generated labels.
More than law‐abiding: A multi‐staged consumer study on brand morality
Authors: Wei Yunyi , Ekinci Yuksel , Sit Kokho (Jason) 
Published: October 2024

AbstractToday's consumers expect brands to act morally to win their hearts and wallets. Yet, existing marketing literature provides little clarity on what this behavior entails and how it differs from acting ethically. This study aims to clarify these concepts and inspire further research. Our consumer‐centric research adopted a multi‐theory and multi‐method approach, coupling Moral Foundations with Morality as Cooperation theories, 12 in‐depth interviews and three surveys with 1229 respondents from two opposite national cultures. We present a Brand Morality scale involving three meaningful dimensions: brand care, brand compliance and brand competency. Brand care is more influential in establishing a moralistic image than the other dimensions. We also learn that while consumers from different national cultures may value the same set of BM dimensions on a cognitive level, they may interpret and react differently on a behavioral‐intent level. Our unique contributions include (i) presenting a 12‐item Brand Morality scale that is theoretically meaningful and practically applicable to brands, (ii) demonstrating the co‐utility of two under‐considered theories, (iii) clarifying the nuanced distinctions of brands acting morally versus ethically, and (iv) shedding light on consumers' perceptual definitions of BM may be consistent between nations, but their behavioral interpretations may vary depending on their local cultures.
The metagenomic landscape of a high‐altitude geothermal spring in Tajikistan reveals a novel Desulfurococcaceae member, Zestomicrobium tamdykulense gen. nov., sp. nov
Authors: Dzhuraeva Munavvara , Bobodzhanova Khursheda , Birkeland Nils‐Kåre 
Published: October 2024

AbstractMetagenomic analysis was conducted to assess the microbial community in the high‐altitude Tamdykul geothermal spring in Tajikistan. This analysis yielded six high‐quality bins from the members of Thermaceae, Aquificaceae, and Halothiobacillaceae, with a 41.2%, 19.7%, and 18.1% share in the total metagenome, respectively. Minor components included Schleiferia thermophila (1.6%) and members of the archaeal taxa Pyrobaculum (1.2%) and Desulfurococcaceae (0.7%). Further analysis of the metagenome‐assembled genome (MAG) from the Desulfurococcaceae family (MAG002) revealed novel taxonomy with only 80.95% closest placement average nucleotide identity value to its most closely related member of the Desulfurococcaceae family, which is part of the Thermoproteota phylum comprising hyperthermophilic members widespread in geothermal environments. MAG002 consisted of 1.3 Mbp, distributed into 48 contigs with 1504 predicted coding sequences, had an average GC content of 41.3%, a completeness and contamination rate of 98.7% and 2.6%, respectively, and branched phylogenetically between the Ignisphaera and Zestosphaera lineages. Digital DNA‐DNA hybridization values compared with Ignisphaera aggregans and Zestosphaera tikiterensis were 33.7% and 19.4%, respectively, suggesting that this MAG represented a novel species and genus. Its 16S rRNA gene contained a large 421 bp intron. It encodes a complete gluconeogenesis pathway involving a bifunctional fructose‐1,6‐bisphosphate phosphatase/aldolase; however, the glycolysis pathway is incomplete. The ribulose monophosphate pathway enzymes could be used for pentose synthesis. MAG002 encodes several hydrogen‐evolving hydrogenases, with possible roles as hydrogen sinks during fermentation. We propose the name Zestomicrobium tamdykulense gen. nov. sp. nov. for this organism; it is the first thermophilic genome reported from Tajikistan.
Characterizing Genetic Susceptibility to Colorectal Cancer in Taiwan Through Genome‐Wide Association Study
Authors: Bau Da‐Tian , Liu Ting‐Yuan , Yang Jai‐Sing , Chen William Tzu‐Liang , Tsai Chia‐Wen , Chang Wen‐Shin , Ke Tao‐Wei , Liao Chi‐Chou , Chen Yu‐Chia , Chang Yen‐Ting , Tsai Fuu‐Jen 
Published: October 2024

ABSTRACTWe conducted the first genome‐wide association study (GWAS) of colorectal cancer (CRC) in Taiwan with 5342 cases and 61,015 controls. Ninety‐two SNPs in three genomic regions reached genome‐wide significance (p < 5 × 10−8). The lead SNPs in these three regions were: rs12778523 (OR = 1.18, 95% CI, 1.15–1.23, p = 4.51 × 10−13), an intergenic SNP between RNA5SP299 and LINC02676 at chromosome 10p14; rs647161 (OR = 1.14, 95% CI, 1.09–1.19, p = 2.21 × 10−9), an intronic SNP in PITX1 at 5q31.1, and rs10427139 (OR = 1.20, 95% CI, 1.14–1.28, p = 3.62 × 10−9), an intronic SNP in GPATCH1 at 19q13.1. We further validated CRC susceptibility SNPs previously identified through GWAS in other populations. A total of 61 CRC susceptibility SNPs were confirmed in Taiwanese. The top validated putative CRC susceptibility genes included: POU2AF2, HAO1, LAMC1, EIF3H, BMP2, ZMIZ1, BMP4, POLD3, CDKN1A, PREX1, CDKN2B, CDH1, and LRIG1. The top enriched pathways included TGF‐β signaling, BMP signaling, extracellular matrix organization, DNA repair, and cell cycle control. We could not validate SNPs in HLA‐G at 6p22.1 and in NOTCH4 at 6p21.32. We generated a weighted genetic risk score (GRS) using the 61 SNPs and constructed receiver operating characteristic (ROC) curves using the GRS to predict CRC. The area under the ROC curve (AUC) was 0.589 for GRS alone and 0.645 for GRS, sex, and age. These susceptibility SNPs and genes provide important insights into the molecular mechanisms of CRC development and help identify high‐risk individuals for CRC in Taiwan.
The STarT Back Screening Tool: The Nepali Translation, Cross‐Cultural Adaptation and Measurement Properties in Adults With Non‐Specific Low Back Pain
Authors: Maharjan Riju , Bovonsunthonchai Sunee , Vachalathiti Roongtiwa , Basnet Ritu , Pathak Anupa , Hill Jonathan , Sharma Saurab 
Published: October 2024

ABSTRACTIntroduction/ObjectiveThe STarT Back Screening Tool (SBST) stratifies low back pain (LBP) patients based on their risk of chronicity to guide treatment accordingly. The absence of its validated Nepali version limits stratified LBP care in Nepal. The study aimed to translate and cross‐culturally adapt the SBST into Nepali and evaluate its measurement properties in adults with LBP.MethodsThe measurement properties of the Nepali SBST were evaluated in 102 Nepali adults with non‐specific LBP. We assessed content validity, internal consistency, test‐retest reliability, construct and discriminant validity. Item redundancy was evaluated using Cronbach's alpha (α > 0.90), test‐retest reliability using Intraclass Correlation Coefficient (ICC2,1) and Cohen's kappa using established cutoffs score for categorising patients into risk groups, construct validity using hypothesis testing (if a minimum of 75% of the hypotheses were supported), and discriminant validity using Area Under the Curve (AUC) with the reference scales administered at baseline.ResultsCronbach's alpha scores were 0.72 for the overall scale and 0.66 for the psychosocial subscale. Test‐retest reliability values were good to excellent with ICC2,1 of 0.94 (95% CI: 0.87–0.97) for the overall scale and 0.87 (95% CI: 0.73–0.94) for the psychosocial subscale and Kappa values of 0.68 (95% CI: 0.43–0.93) for the overall scale and 0.79 (95% CI: 0.52–1.00) for psychosocial subscale. Construct validity was confirmed as 100% of a priori hypotheses were met. Acceptable discriminative validity was observed with reference scales with AUCs (0.75–0.80).ConclusionsNepali SBST demonstrates the reliability and validity of screening for chronicity risk in Nepali adults with LBP. Future studies should evaluate its responsiveness, predictive abilities, and effectiveness in stratifying LBP patients in the Nepalese context.