bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Composition and Sensory Quality of Tunisian ‘Sayali’ Virgin Olive Oils as Affected by Fruit Ripening: Toward an Appropriate Harvesting Time
Authors: Nsir Houda , Taamalli Amani , Valli Enrico , Bendini Alessandra , Gallina Toschi Tullia , Zarrouk Mokhtar 
Published: June 2017

AbstractHerein, the influence of the ripeness of Tunisian Sayali olives on the chemical composition and sensory quality of virgin olive oils have been investigated, with a particular focus on minor metabolites. Towards this end, five samples (S1–S5) were produced from fruits at increasing stages of maturity and then analyzed. Quality indices (free acidity, peroxide value, specific extinction in UV, sensory characteristics) and composition in major (fatty acids) and minor compounds (squalene, pigments, tocopherols, phenolic compounds, volatile compounds), as well as oxidative stability, were evaluated. Significant variations for the most analytical parameters of Sayali samples were demonstrated, highlighting the impact of stage of ripening. In particular, at later stages of ripening a decreased tendency was seen in minor compounds that are able to inhibit lipid oxidation (tocopherols, carotenoids, squalene and polar phenolic compounds) and, as a consequence, in the oxidative stability value. Moreover, a higher intensity of positive sensory notes (fruity, bitter and pungent) characteristic of extra virgin olive oil were found for samples produced with less ripe olives. Finally, a deeper knowledge of the influence of this factor would be helpful to correctly manage the optimal fruit harvesting time for producers for this variety and to improve the marketing of extra virgin olive oils by using a promising secondary variety (with a high oxidative stability and an interesting fatty acid composition).
Absolute Dating of the Gihon Spring Fortifications, Jerusalem
Authors: Regev Johanna , Uziel Joe , Szanton Nahshon , Boaretto Elisabetta 
Published: June 2017

AbstractOne of the most impressive structures in Jerusalem’s ancient landscape is the tower that was built to surround and protect the Gihon Spring, Jerusalem’s perennial water source. The structure, first discovered by Reich and Shukron (2004), encompasses the cave in which the spring sprouts from, with walls 7 m thick built of large boulders. The Spring Tower, along with the other features relating to it, were all attributed to the Middle Bronze Age II, based on their architectural and stratigraphical relationship, the type of architecture, and the pottery found. In the continued excavations carried out by the Israel Antiquities Authority along the outer, eastern face of the Spring Tower, it was noted that at least the northeast side of the tower was not built on bedrock, but rather on layers of sediment, which were sealed by the massive boulders at the base of the tower. In order to provide an absolute dating for the structure, two sections were sampled for radiocarbon (14C) dating beneath the foundation stones at two locations. Scenarios for the construction of the tower during Middle Bronze Age (MB) and Iron Age II are considered, based on the new14C data, yielding a series of dates, the latest of which falls in the terminal phases of the 9th century BCE, alongside previous excavation data.
Isotopic Evidence for Environmental Adaptation in Medieval Iin Hamina, Northern Finland
Authors: Lahtinen Maria 
Published: June 2017

AbstractThis study investigates human environmental adaptation in northern Finland at the cemetery of Iin Hamina. The cemetery was in use in the 15th and 16th centuries AD during the cool climate anomaly called the Little Ice Age. It is possible that these extreme climatic conditions may have impacted human survival in this agriculturally marginal region. In previous studies, carbon and nitrogen isotopes in the bone collagen of humans and fauna have shown that the main protein source for people at Iin Hamina was fish. In this study, I observe annual changes in diet derived from isotopic studies of teeth. Apart from one individual whose protein source shifted radically during tooth formation, the results show the inhabitants of Iin Hamina experienced only minor changes in diet over periods of several years. Moreover, none of the dietary profiles provided evidence of dietary or physiological stress through raised nitrogen isotope ratios. The results indicate that the individuals in this study appear to have been well-adapted to their environment.
Waist circumference as a predictor of mortality in peritoneal dialysis patients: a follow-up study of 48 months
Authors: Castro Ana Catarina M. , Bazanelli Ana Paula , Nerbass Fabiana B. , Cuppari Lilian , Kamimura Maria A. 
Published: June 2017

AbstractBody-fat gain is a common finding among peritoneal dialysis (PD) patients, and the accumulation of adipose tissue occurs predominantly in the abdominal area. Waist circumference (WC) is a reliable marker of abdominal obesity and its association with worse outcomes has been demonstrated in non-dialysis and haemodialysis patients. We aimed at investigating whether WC measurements as well as the changes over time in WC were able to predict mortality in PD patients. This prospective study included 109 patients undergoing PD (57 % male, age 52 (sd16) years, 32 % diabetics, 48 % BMI≥25 kg/m2). WC was measured at the umbilicus level (empty abdominal cavity), and values >88 cm for women and >102 cm for men were considered high. Nutritional status and laboratory parameters were also evaluated. WC was measured at baseline and after 6 months, and mortality was registered during a period of 48 months. High WC was observed in 55 % of women and in 23 % of men at baseline. After 6 months, 61 % of the patients showed an increased WC. At the end of the study, twenty-seven deaths were registered. A significant increase in WC was observed only in the non-survivor group. In the Cox regression analysis adjusting for sex, age, duration on dialysis, diabetes, BMI, serum albumin and C-reactive protein, high WC at baseline as well as the 6-month increase in WC were independently associated with mortality. This study demonstrated that a high WC and the increase over time in WC were both predictors of mortality in PD patients.
RELIABILITY OF NDVI DERIVED BY HIGH RESOLUTION SATELLITE AND UAV COMPARED TO IN-FIELD METHODS FOR THE EVALUATION OF EARLY CROP N STATUS AND GRAIN YIELD IN WHEAT
Authors: BENINCASA PAOLO , ANTOGNELLI SARA , BRUNETTI LUCA , FABBRI CARLO ALBERTO , NATALE ANTONIO , SARTORETTI VELIA , MODEO GIANLUCA , GUIDUCCI MARCELLO , TEI FRANCESCO , VIZZARI MARCO 
Published: June 2017

SUMMARYThis study was aimed at comparing in-field parameters and remote sensing NDVI (normalized difference vegetation index) by both satellite (SAT) and unmanned aerial vehicle (UAV) for the assessment of early nitrogen (N) status and prediction of yield in winter wheat (Triticum aestivum L.). Six increasing N rates, i.e., 0, 40, 80, 120, 160, 200 kg N ha−1 were applied, half at tillering and half at shooting. Thus, when the crop N status was monitored between the two N applications, consecutive N treatments differentiated from each other by just 20 kg N ha−1. The following in-field and remote sensed parameters were compared as indicators of crop vegetative and N status: plant N% (w:w) concentration; crop N uptake (Nupt); ratio between transmitted and incident photosynthetically active radiation (PARt/PARi); leaf SPAD values, an indirect index for chlorophyll content; SAT and UAV derived NDVI. As reliable indicators of wheat N availability, in-field parameters were ranked as follows: PARt/PARi ≅ Nupt > SPAD ≅ N%. The PARt/PARi, Nupt and SPAD resulted quite strongly correlated to each other. At all crop stages, the NDVI was strongly correlated with PARt/PARi and Nupt. It is of relevance that NDVI correlated quite strongly to in-field parameters and grain yield at shooting, i.e., before the second N application, when the N rate can still be adjusted. The SAT and UAV NDVIs were strongly correlated to each other, which means they can be used alternatively depending on the context.
Fine mapping by composite genome-wide association analysis
Authors: CASELLAS JOAQUIM , CAÑAS-ÁLVAREZ JHON JACOBO , FINA MARTA , PIEDRAFITA JESÚS , CECCHINATO ALESSIO 
Published: June 2017

SummaryGenome-wide association (GWA) studies play a key role in current genetics research, unravelling genomic regions linked to phenotypic traits of interest in multiple species. Nevertheless, the extent of linkage disequilibrium (LD) may provide confounding results when significant genetic markers span along several contiguous cM. In this study, we have adapted the composite interval mapping approach to the GWA framework (composite GWA), in order to evaluate the impact of including competing (possibly linked) genetic markers when testing for the additive allelic effect inherent to a given genetic marker. We tested model performance on simulated data sets under different scenarios (i.e., qualitative trait loci effects, LD between genetic markers and width of the genomic region involved in the analysis). Our results showed that the genomic region had a small impact on the number of competing single nucleotide polymorphisms (SNPs) as well as on the precision of the composite GWA analysis. A similar conclusion was derived from the preferable range of LD between the tested SNP and competing SNPs, although moderate-to-high LD seemed to attenuate the loss of statistical power. The composite GWA improved specificity and reduced the number of significant genetic markers. The composite GWA model contributes a novel point of view for GWA analyses where testing circumscribed to the genomic region flanking each SNP (delimited by the nearest competing SNPs) and conditioning on linked markers increases the precision to locate causal mutations, but possibly at the expense of power.
The Jurassic–Early Cretaceous basalt–chert association in the ophiolites of the Ankara Mélange, east of Ankara, Turkey: age and geochemistry
Authors: BORTOLOTTI VALERIO , CHIARI MARCO , GÖNCÜOGLU M. CEMAL , PRINCIPI GIANFRANCO , SACCANI EMILIO , TEKIN U. KAGAN , TASSINARI RENZO 
Published: June 2017

AbstractThis study is focused on slide blocks including oceanic lavas associated with pelagic sediments within the eastern part of the Ankara Mélange. A detailed petrological characterization of the volcanic rocks and a detailed biochronological investigation of the associated radiolarian cherts in eight sections (east of Ankara) was carried out. The volcanic rocks are largely represented by basalts and minor ferrobasalts and trachytes. They show different geochemical affinities and overlapping ages including: (a) Late Jurassic – Early Cretaceous garnet-influenced MORB (middle late Oxfordian to late Kimmeridgian–early Tithonian and early–early late Tithonian; late Valanginian–early Barremian); (b) Early Cretaceous enriched-MORB (middle late Barremian–early early Aptian; Valanginian to middle Aptian–early Albian); (c) Middle Jurassic plume-type MORB (early–middle Bajocian to late Bathonian–early Callovian); (d) Late Jurassic – Early Cretaceous alkaline basalts (middle–late Oxfordian to late Kimmeridgian–early Tithonian; late Valanginian to late Hauterivian). All rock types show a clear garnet signature, as testified to by their high MREE/HREE (middle rare earth element/heavy rare earth element) ratios. The coexistence of chemically different rock types from Middle Jurassic to Early Cretaceous times suggests that they were formed in a mid-ocean ridge setting from partial melting of a highly heterogeneous mantle characterized by the extensive occurrence of OIB-metasomatized portions, which were likely inherited from Triassic mantle plume activity associated with the continental rift and opening of the Neotethys branch.
Long-read sequencing improves assembly of Trichinella genomes 10-fold, revealing substantial synteny between lineages diverged over 7 million years
Authors: THOMPSON PETER C. , ZARLENGA DANTE S. , LIU MING-YUAN , ROSENTHAL BENJAMIN M. 
Published: June 2017

SUMMARYGenome assemblies can form the basis of comparative analyses fostering insight into the evolutionary genetics of a parasite's pathogenicity, host–pathogen interactions, environmental constraints and invasion biology; however, the length and complexity of many parasite genomes has hampered the development of well-resolved assemblies. In order to improve Trichinella genome assemblies, the genome of the sylvatic encapsulated species Trichinella murrelli was sequenced using third-generation, long-read technology and, using syntenic comparisons, scaffolded to a reference genome assembly of Trichinella spiralis, markedly improving both. A high-quality draft assembly for T. murrelli was achieved that totalled 63·2 Mbp, half of which was condensed into 26 contigs each longer than 571 000 bp. When compared with previous assemblies for parasites in the genus, ours required 10-fold fewer contigs, which were five times longer, on average. Better assembly across repetitive regions also enabled resolution of 8 Mbp of previously indeterminate sequence. Furthermore, syntenic comparisons identified widespread scaffold misassemblies in the T. spiralis reference genome. The two new assemblies, organized for the first time into three chromosomal scaffolds, will be valuable resources for future studies linking phenotypic traits within each species to their underlying genetic bases.
The role of rare innate immune cells in Type 2 immune activation against parasitic helminths
Authors: WEBB LAUREN M. , TAIT WOJNO ELIA D. 
Published: June 2017

SUMMARYThe complexity of helminth macroparasites is reflected in the intricate network of host cell types that participate in the Type 2 immune response needed to battle these organisms. In this context, adaptive T helper 2 cells and the Type 2 cytokines interleukin (IL)-4, IL-5, IL-9 and IL-13 have been the focus of research for years, but recent work has demonstrated that the innate immune system plays an essential role. Some innate immune cells that promote Type 2 immunity are relatively abundant, such as macrophages and eosinophils. However, we now appreciate that more rare cell types including group 2 innate lymphoid cells, basophils, mast cells and dendritic cells make significant contributions to these responses. These cells are found at low frequency but they are specialized to their roles – located at sites such as the skin, lung and gut, where the host combats helminth parasites. These cells respond rapidly and robustly to worm antigens and worm-induced damage to produce essential cytokines, chemokines, eicosanoids and histamine to activate damaged epithelium and to recruit other effectors. Thus, a greater understanding of how these cells operate is essential to understand how the host protects itself during helminth infection.
Anti-parasitic effect of the diuretic and Na+-ATPAse inhibitor furosemide in cutaneous leishmaniasis
Authors: ARRUDA-COSTA N. , ESCRIVANI D. , ALMEIDA-AMARAL E. E. , MEYER-FERNANDES J. R. , ROSSI-BERGMANN B. 
Published: June 2017

SUMMARYLeishmania amazonensispromastigotes are known to express furosemide (Lasix®)-sensitiveP-type membrane Na+-ATPase. In the present study, furosemide activity was studied in intracellular amastigotes and infected BALB/c mice to investigate its efficacy in cutaneous leishmaniasis (CL). Intracellular parasites, but not macrophages, were found to be sensitive to killing by furosemide (IC50= 87µmvsCC50≫ 1000µm, respectively). Although furosemide did not induce nitric oxide production or intracellular pH changes in infected macrophages, it led to a significant reactive oxygen species (ROS) burst. Freshly isolated tissue parasites expressed a high degree of Na+-ATPase activity that decreased with culture, indicative of a higher enzyme expression in amastigotes than in promastigotes. Both intraperitoneal and oral treatment ofL. amazonensis-infected mice with furosemide dosages equivalent to that prescribed as a diuretic significantly reduced the parasite's growth compared with the situation in untreated mice. Combination with oral furosemide increased the efficacy and safety of intraperitoneal treatment with sodium stibogluconate (SSG). To summarize, furosemide control of intracellular leishmanial growth by means of parasite Na+-ATPase inhibition, and macrophage ROS activation may help explain its sole and SSG-combined therapeutic effect against murine CL.
Trichomonas tenax and periodontal diseases: a concise review
Authors: MARTY MATHIEU , LEMAITRE MATHIEU , KÉMOUN PHILIPPE , MORRIER JEAN-JACQUES , MONSARRAT PAUL 
Published: June 2017

SUMMARYPeriodontal diseases (gingivitis and periodontitis), result from a disruption of the host–oral microbiome homoeostasis. Whereas the pathological role of some specific bacterial strains during periodontal diseases is well documented, the impact of parasites in periodontium pathophysiology is still under debate. This review aims to collect data about the prevalence and the potential role of Trichomonas tenax during periodontal diseases. Data from 47 studies revealed that T. tenax prevalence in diseased periodontium ranged from 0 to 94·1%. The prevalence of oral protozoan infections was found to be largely greater in patients with periodontal diseases than with healthy periodontium. The parasite detection was mainly performed by direct microscopy. Trichomonas tenax presence was clearly correlated with periodontal disease. The high heterogeneity of its periodontal prevalence may be correlated with the diversity of the population screened (age, sex, systemic diseases), and the methods used for diagnosis. This protozoan seems to have the capacity to be involved in the inflammatory process of gum disease. Animal experimentation, using relevant physiopathological models of periodontitis, needs to be performed to investigate the ability of T. tenax to cause and/or worsen the disease. Further investigations using standardized experimental designs of epidemiologic studies are also needed.
Demodex canis regulates cholinergic system mediated immunosuppressive pathways in canine demodicosis
Authors: KUMARI P. , NIGAM R. , SINGH A. , NAKADE U. P. , SHARMA A. , GARG S. K. , SINGH S. K. 
Published: June 2017

SUMMARYDemodex canis infestation in dogs remains one of the main challenges in veterinary dermatology. The exact pathogenesis of canine demodicosis is unknown but an aberration in immune status is considered very significant. No studies have underpinned the nexus between induction of demodicosis and neural immunosuppressive pathways so far. We have evaluated the involvement of cholinergic pathways in association with cytokines regulation as an insight into the immuno-pathogenesis of canine demodicosis in the present study. Remarkable elevations in circulatory immunosuppressive cytokine interleukin-10 and cholinesterase activity were observed in dogs with demodicosis. Simultaneously, remarkable reduction in circulatory pro-inflammatory cytokine tumour necrosis factor-alpha level was observed in dogs with demodicosis. Findings of the present study evidently suggest that Demodex mites might be affecting the cholinergic pathways to induce immunosuppression in their host and then proliferate incessantly in skin microenvironment to cause demodicosis.
Meta-analysis of longitudinal studies of cognition in bipolar disorder: comparison with healthy controls and schizophrenia
Authors: Bora E. , Özerdem A. 
Published: June 2017

BackgroundBipolar disorder (BP) is associated with significant cognitive impairment. Recent evidence suggests that cognitive deficits are already evident after first-episode mania. However, it is not clear whether BP is associated with further decline in cognitive functions in individuals with established illness. Aim of this meta-analytic review was to examine longitudinal neurocognitive changes in BP and to compare trajectory of cognitive deficits in BP with schizophrenia and healthy controls.MethodsElectronic databases were searched for the studies published between January 1987 and November 2016. In total 22 reports were included in the current meta-analysis. The main analysis assessed the longitudinal change in cognition in 643 patients with BP. Further analyses were conducted in studies investigating cognitive changes in BP along with healthy controls (459 BP and 367 healthy controls) and schizophrenia (172 BP and 168 schizophrenia).ResultsThere was no cognitive decline overtime neither in short-term (mean duration = 1.5 years) nor in long-term (mean duration = 5.5 years) follow-up studies in BP. In contrast, there was evidence for modest improvements in task performance in memory and working memory at follow-up. The trajectory of cognitive functioning in BP was not significantly different from changes in schizophrenia and healthy controls.ConclusionsTogether with the findings in early BP and individuals at genetic risk for BP, current findings suggest that neurodevelopmental factors might play a significant role in cognitive deficits in BP and do not support the notion of progressive cognitive decline in most patients with BP.
Lysozyme's lectin-like characteristics facilitates its immune defense function
Authors: Zhang Ruiyan , Wu Lisha , Eckert Thomas , Burg-Roderfeld Monika , Rojas-Macias Miguel A. , Lütteke Thomas , Krylov Vadim B. , Argunov Dmitry A. , Datta Aritreyee , Markart Philipp , Guenther Andreas , Norden Bengt , Schauer Roland , Bhunia Anirban , Enani Mushira Abdelaziz , Billeter Martin , Scheidig Axel J. , Nifantiev Nikolay E. , Siebert Hans-Christian 
Published: June 2017

Abstract
Patterns of biogenic weathering as indicators of palaeoclimates in Israel
Authors: Danin Avinoam 
Published: June 2017

SynopsisMassive calcareous rocks and stones support lithobiont communities of bacteria, fungi, cyanobacteria, lichens, and phanerogams in most parts of Israel. The distribution of these communities is influenced by environmental conditions. Moisture regime of their substratum is the most important factor. Many of the lithobiont communities cause typical patterns of weathering that may be preserved on the rock surface or inside the rock for thousands of years. Knowing the climatic affinities of the lithobiont communities, one can use the uncoveredin siturocks and stones and those that were buried in the past as fossil climate recorders. With this method, the following deductions have been made about palaeoclimates in our area: (i) the wettest period ended 30,000 years BP; (ii) a period drier than the present took place sometime between 10,000 and 30,000 years BP; (iii) the Neolithic Period (9,000–10,000 years BP) was much wetter than the present; (iv) since the Chalcolithic Period (5,000 to 6,000 years BP) the climate of Israel has been similar to that of the present.
Denis Burkitt and the origins of the dietary fibre hypothesis
Authors: Cummings John H. , Engineer Amanda 
Published: June 2017

AbstractFor more than 200 years the fibre in plant foods has been known by animal nutritionists to have significant effects on digestion. Its role in human nutrition began to be investigated towards the end of the 19th century. However, between 1966 and 1972, Denis Burkitt, a surgeon who had recently returned from Africa, brought together ideas from a range of disciplines together with observations from his own experience to propose a radical view of the role of fibre in human health. Burkitt came late to the fibre story but built on the work of three physicians (Peter Cleave, G. D. Campbell and Hugh Trowell), a surgeon (Neil Painter) and a biochemist (Alec Walker) to propose that diets low in fibre increase the risk of CHD, obesity, diabetes, dental caries, various vascular disorders and large bowel conditions such as cancer, appendicitis and diverticulosis. Simply grouping these diseases together as having a common cause was groundbreaking. Proposing fibre as the key stimulated much research but also controversy. Credit for the dietary fibre hypothesis is given largely to Burkitt who became known as the ‘Fibre Man’. This paper sets out the story of the development of the fibre hypothesis, and the contribution to it of these individuals.