bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-Glycosylation of integrin α5 acts as a switch for EGFR-mediated complex formation of integrin α5β1 to α6β4
Authors: Hang Qinglei , Isaji Tomoya , Hou Sicong , Zhou Ying , Fukuda Tomohiko , Gu Jianguo 
Published: September 2016

AbstractN-Glycosylation of integrin α5β1 is involved in multiple cell behaviors. We previously reported that the N-glycosylations of the calf domain on integrin α5 (S3–5,10–14) are essential for its inhibitory effect on EGFR signaling in regulating cell proliferation. However, the importance of the individual N-glycosylation and the underlying mechanisms of inhibition remain unclear. Here, we characterize the S3–5,10–14 mutants in detail and found that the N-glycosylation of site-11 (Asn712) is key for cell growth. The restoration of site-11, unlike the other individual sites, significantly suppressed cell growth and EGFR signaling in a manner that was similar to that of wild-type (WT). Mechanistically, this N-glycosylation inhibited the response abilities upon EGF stimulation and EGFR dimerization. Interestingly, we found this N-glycosylation controlled the EGFR complex formation with integrin α5β1 or α6β4; i.e., the loss of site-11 switched EGFR-α5β1 to EGFR-α6β4, which is well known to promote cellular signaling for cell growth. Moreover, the site-11 N-glycan exhibited a more branching structure compared with other sites, which may be required for EGFR-α5β1 formation. Taken together, these data clearly demonstrate that the site-11 N-glycosylation on α5 is most important for its inhibitory effect on EGFR signaling, which may provide a novel regulatory mechanism for crosstalks between integrins and EGFR.
Flexible Neural Electrode Array Based-on Porous Graphene for Cortical Microstimulation and Sensing
Authors: Lu Yichen , Lyu Hongming , Richardson Andrew G. , Lucas Timothy H. , Kuzum Duygu 
Published: September 2016

AbstractNeural sensing and stimulation have been the backbone of neuroscience research, brain-machine interfaces and clinical neuromodulation therapies for decades. To-date, most of the neural stimulation systems have relied on sharp metal microelectrodes with poor electrochemical properties that induce extensive damage to the tissue and significantly degrade the long-term stability of implantable systems. Here, we demonstrate a flexible cortical microelectrode array based on porous graphene, which is capable of efficient electrophysiological sensing and stimulation from the brain surface, without penetrating into the tissue. Porous graphene electrodes show superior impedance and charge injection characteristics making them ideal for high efficiency cortical sensing and stimulation. They exhibit no physical delamination or degradation even after 1 million biphasic stimulation cycles, confirming high endurance. In in vivo experiments with rodents, same array is used to sense brain activity patterns with high spatio-temporal resolution and to control leg muscles with high-precision electrical stimulation from the cortical surface. Flexible porous graphene array offers a minimally invasive but high efficiency neuromodulation scheme with potential applications in cortical mapping, brain-computer interfaces, treatment of neurological disorders, where high resolution and simultaneous recording and stimulation of neural activity are crucial.
Tin doped indium oxide anodes with artificially controlled nano-scale roughness using segregated Ag nanoparticles for organic solar cells
Authors: Kim Hyo-Joong , Ko Eun-Hye , Noh Yong-Jin , Na Seok-In , Kim Han-Ki 
Published: September 2016

AbstractNano-scale surface roughness in transparent ITO films was artificially formed by sputtering a mixed Ag and ITO layer and wet etching of segregated Ag nanoparticles from the surface of the ITO film. Effective removal of self-segregated Ag particles from the grain boundaries and surface of the crystalline ITO film led to a change in only the nano-scale surface morphology of ITO film without changes in the sheet resistance and optical transmittance. A nano-scale rough surface of the ITO film led to an increase in contact area between the hole transport layer and the ITO anode, and eventually increased the hole extraction efficiency in the organic solar cells (OSCs). The heterojunction OSCs fabricated on the ITO anode with a nano-scale surface roughness exhibited a higher power conversion efficiency of 3.320%, than that (2.938%) of OSCs made with the reference ITO/glass. The results here introduce a new method to improve the performance of OSCs by simply modifying the surface morphology of the ITO anodes.
A simple and cost-saving phenotypic drug susceptibility testing of HIV-1
Authors: Weng Yunceng , Zhang Ling , Huang Jianfeng , Zhao Jin , Luo Peifang , Bi Siyuan , Yang Zhengrong , Zhu Hai , Allain Jean-Pierre , Li Chengyao 
Published: September 2016

AbstractIt is essential to monitor the occurrence of drug-resistant strains and to provide guidance for clinically adapted antiviral treatment of HIV/AIDS. In this study, an individual patient’s HIV-1 pol gene encoding the full length of protease and part of the reverse transcriptase was packaged into a modified lentivirus carrying dual-reporters ZsGreen and luciferase. The optimal coefficient of correlation between drug concentration and luciferase activity was optimized. A clear-cut dose-dependent relationship between lentivirus production and luciferase activity was found in the phenotypic testing system. Fold changes (FC) to a wild-type control HIV-1 strain ratios were determined reflecting the phenotypic susceptibility of treatment-exposed patient’s HIV-1 strains to 12 HIV-1 inhibitors including 6 nucleoside reverse-transcriptase inhibitors (NRTIs), 4 non-nucleoside reverse transcriptase inhibitors (NNRTIs) and 2 protease inhibitors (PIs). Phenotypic susceptibility calls from 8 HIV-1 infected patients were consistent with 80–90% genotypic evaluations, while phenotypic assessments rectified 10–20% genotypic resistance calls. By a half of replacement with ZsGreen reporter, the consumption of high cost Bright-Glo Luciferase Assay is reduced, making this assay cheaper when a large number of HIV-1 infected individuals are tested. The study provides a useful tool for interpreting meaningful genotypic mutations and guiding tailored antiviral treatment of HIV/AIDS in clinical practice.
High-Resolution Imaging by Adaptive Optics Scanning Laser Ophthalmoscopy Reveals Two Morphologically Distinct Types of Retinal Hard Exudates
Authors: Yamaguchi Muneo , Nakao Shintaro , Kaizu Yoshihiro , Kobayashi Yoshiyuki , Nakama Takahito , Arima Mitsuru , Yoshida Shigeo , Oshima Yuji , Takeda Atsunobu , Ikeda Yasuhiro , Mukai Shizuo , Ishibashi Tatsuro , Sonoda Koh-hei 
Published: September 2016

AbstractHistological studies from autopsy specimens have characterized hard exudates as a composition of lipid-laden macrophages or noncellular materials including lipid and proteinaceous substances (hyaline substances). However, the characteristics of hard exudates in living patients have not been examined due to insufficient resolution of existing equipment. In this study, we used adaptive optics scanning laser ophthalmoscopy (AO-SLO) to examine the characteristics of hard exudates in patients with retinal vascular diseases. High resolution imaging using AO-SLO enables morphological classification of retinal hard exudates into two types, which could not be distinguished either on fundus examination or by spectral domain optical coherence tomography (SD-OCT). One, termed a round type, consisted of an accumulation of spherical particles (average diameter of particles: 26.9 ± 4.4 μm). The other, termed an irregular type, comprised an irregularly shaped hyper-reflective deposition. The retinal thickness in regions with round hard exudates was significantly greater than the thickness in regions with irregular hard exudates (P = 0.02). This differentiation of retinal hard exudates in patients by AO-SLO may help in understanding the pathogenesis and clinical prognosis of retinal vascular diseases.
A mixed solution-processed gate dielectric for zinc-tin oxide thin-film transistor and its MIS capacitance
Authors: Kim Hunho , Kwack Young-Jin , Yun Eui-Jung , Choi Woon-Seop 
Published: September 2016

AbstractSolution-processed gate dielectrics were fabricated with the combined ZrO2 and Al2O3 (ZAO) in the form of mixed and stacked types for oxide thin film transistors (TFTs). ZAO thin films prepared with double coatings for solid gate dielectrics were characterized by analytical tools. For the first time, the capacitance of the oxide semiconductor was extracted from the capacitance-voltage properties of the zinc-tin oxide (ZTO) TFTs with the combined ZAO dielectrics by using the proposed metal-insulator-semiconductor (MIS) structure model. The capacitance evolution of the semiconductor from the TFT model structure described well the threshold voltage shift observed in the ZTO TFT with the ZAO (1:2) gate dielectric. The electrical properties of the ZTO TFT with a ZAO (1:2) gate dielectric showed low voltage driving with a field effect mobility of 37.01 cm2/Vs, a threshold voltage of 2.00 V, an on-to-off current ratio of 1.46 × 105, and a subthreshold slope of 0.10 V/dec.
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
Authors: Munk Michele , Ladeira Luiz O. , Carvalho Bruno C. , Camargo Luiz S. A. , Raposo Nádia R. B. , Serapião Raquel V. , Quintão Carolina C. R. , Silva Saulo R. , Soares Jaqueline S. , Jorio Ado , Brandão Humberto M. 
Published: September 2016

AbstractThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
A contaminant-free assessment of Endogenous Retroviral RNA in human plasma
Authors: Karamitros Timokratis , Paraskevis Dimitrios , Hatzakis Angelos , Psichogiou Mina , Elefsiniotis Ioannis , Hurst Tara , Geretti Anna-Maria , Beloukas Apostolos , Frater John , Klenerman Paul , Katzourakis Aris , Magiorkinis Gkikas 
Published: September 2016

AbstractEndogenous retroviruses (ERVs) comprise 6–8% of the human genome. HERVs are silenced in most normal tissues, up-regulated in stem cells and in placenta but also in cancer and HIV-1 infection. Crucially, there are conflicting reports on detecting HERV RNA in non-cellular clinical samples such as plasma that suggest the study of HERV RNA can be daunting. Indeed, we find that the use of real-time PCR in a quality assured clinical laboratory setting can be sensitive to low-level proviral contamination. We developed a mathematical model for low-level contamination that allowed us to design a laboratory protocol and standard operating procedures for robust measurement of HERV RNA. We focus on one family, HERV-K HML-2 (HK2) that has been most recently active even though they invaded our ancestral genomes almost 30 millions ago. We extensively validated our experimental design on a model cell culture system showing high sensitivity and specificity, totally eliminating the proviral contamination. We then tested 236 plasma samples from patients infected with HIV-1, HCV or HBV and found them to be negative. The study of HERV RNA for human translational studies should be performed with extensively validated protocols and standard operating procedures to control the widespread low-level human DNA contamination.
ATP from synaptic terminals and astrocytes regulates NMDA receptors and synaptic plasticity through PSD-95 multi-protein complex
Authors: Lalo U. , Palygin O. , Verkhratsky A. , Grant S. G. N. , Pankratov Y. 
Published: September 2016

AbstractRecent studies highlighted the importance of astrocyte-secreted molecules, such as ATP, for the slow modulation of synaptic transmission in central neurones. Biophysical mechanisms underlying the impact of gliotransmitters on the strength of individual synapse remain, however, unclear. Here we show that purinergic P2X receptors can bring significant contribution to the signalling in the individual synaptic boutons. ATP released from astrocytes facilitates a recruitment of P2X receptors into excitatory synapses by Ca2+-dependent mechanism. P2X receptors, co-localized with NMDA receptors in the excitatory synapses, can be activated by ATP co-released with glutamate from pre-synaptic terminals and by glia-derived ATP. An activation of P2X receptors in turn leads to down-regulation of postsynaptic NMDA receptors via Ca2+-dependent de-phosphorylation and interaction with PSD-95 multi-protein complex. Genetic deletion of the PSD-95 or P2X4 receptors obliterated ATP-mediated down-regulation of NMDA receptors. Impairment of purinergic modulation of NMDA receptors in the PSD-95 mutants dramatically decreased the threshold of LTP induction and increased the net magnitude of LTP. Our findings show that synergistic action of glia- and neurone-derived ATP can pre-modulate efficacy of excitatory synapses and thereby can have an important role in the glia-neuron communications and brain meta-plasticity.
Satellite data lift the veil on offshore platforms in the South China Sea
Authors: Liu Yongxue , Sun Chao , Sun Jiaqi , Li Hongyi , Zhan Wenfeng , Yang Yuhao , Zhang Siyu 
Published: September 2016

AbstractOil and gas exploration in the South China Sea (SCS) has garnered global attention recently; however, uncertainty regarding the accurate number of offshore platforms in the SCS, let alone their detailed spatial distribution and dynamic change, may lead to significant misjudgment of the true status of offshore hydrocarbon production in the region. Using both fresh and archived space-borne images with multiple resolutions, we enumerated the number, distribution, and annual rate of increase of offshore platforms across the SCS. Our results show that: (1) a total of 1082 platforms are present in the SCS, mainly located in shallow-water; and (2) offshore oil/gas exploitation in the SCS is increasing in intensity and advancing from shallow to deep water, and even to ultra-deep-water. Nevertheless, our findings suggest that oil and gas exploration in the SCS may have been over-estimated by one-third in previous reports. However, this overestimation does not imply any amelioration of the potential for future maritime disputes, since the rate of increase of platforms in disputed waters is twice that in undisputed waters.
A new insight into the immobilization mechanism of Zn on biochar: the role of anions dissolved from ash
Authors: Qian Tingting , Wang Yujun , Fan Tingting , Fang Guodong , Zhou Dongmei 
Published: September 2016

AbstractBiochar is considered to be a promising material for heavy metal immobilization in soil. However, the immobilization mechanisms of Zn2+ on biochars derived from many common waste biomasses are not completely understood. Herein, biochars (denoted as PN350, PN550, WS350 and WS550) derived from pine needle (PN) and wheat straw (WS) were prepared at two pyrolysis temperatures (350 °C and 550 °C). The immobilization behaviors and mechanisms of Zn2+ on these biochars were systematically investigated. The results show that compared with biochars produced at low temperature, biochars produced at high temperature contained higher amounts of ash and exhibited much higher sorption capacities of Zn2+. By using Zn K-edge EXAFS spectroscopy, we find that the formation of various Zn precipitates/minerals, which was caused by the release of OH−, CO32− and Si species from biochar, was the immobilization mechanism of Zn2+ on PN and WS biochars. Hydrozincite and Zn(OH)2 were the main species formed on PN350, PN550 and WS350; while on WS550, besides hydrozincite, a large fraction of hemimorphite was formed. The occurrence of hydrozincite and hemimorphite on biochar during Zn2+ immobilization is firstly reported in our study, which provides a new insight into the immobilization mechanism of Zn2+ on biochar.
Novel Solvent-free Perovskite Deposition in Fabrication of Normal and Inverted Architectures of Perovskite Solar Cells
Authors: Nejand Bahram Abdollahi , Gharibzadeh Saba , Ahmadi Vahid , Shahverdi H. Reza 
Published: September 2016

AbstractWe introduced a new approach to deposit perovskite layer with no need for dissolving perovskite precursors. Deposition of Solution-free perovskite (SFP) layer is a key method for deposition of perovskite layer on the hole or electron transport layers that are strongly sensitive to perovskite precursors. Using deposition of SFP layer in the perovskite solar cells would extend possibility of using many electron and hole transport materials in both normal and invert architectures of perovskite solar cells. In the present work, we synthesized crystalline perovskite powder followed by successful deposition on TiO2 and cuprous iodide as the non-sensitve and sensitive charge transport layers to PbI2 and CH3NH3I solution in DMF. The post compressing step enhanced the efficiency of the devices by increasing the interface area between perovskite and charge transport layers. The 9.07% and 7.71% cell efficiencies of the device prepared by SFP layer was achieved in respective normal (using TiO2 as a deposition substrate) and inverted structure (using CuI as deposition substrate) of perovskite solar cell. This method can be efficient in large-scale and low cost fabrication of new generation perovskite solar cells.
The Asymmetric Active Coupler: Stable Nonlinear Supermodes and Directed Transport
Authors: Kominis Yannis , Bountis Tassos , Flach Sergej 
Published: September 2016

AbstractWe consider the asymmetric active coupler (AAC) consisting of two coupled dissimilar waveguides with gain and loss. We show that under generic conditions, not restricted by parity-time symmetry, there exist finite-power, constant-intensity nonlinear supermodes (NS), resulting from the balance between gain, loss, nonlinearity, coupling and dissimilarity. The system is shown to possess non-reciprocal dynamics enabling directed power transport functionality.
Resolution criteria in double-slit microscopic imaging experiments
Authors: You Shangting , Kuang Cuifang , Zhang Baile 
Published: September 2016

AbstractDouble-slit imaging is widely used for verifying the resolution of high-resolution and super-resolution microscopies. However, due to the fabrication limits, the slit width is generally non-negligible, which can affect the claimed resolution. In this paper we theoretically calculate the electromagnetic field distribution inside and near the metallic double slit using waveguide mode expansion method and acquire the far-field image by vectorial Fourier optics. We find that the slit width has minimal influence when the illuminating light is polarized parallel to the slits. In this case, the claimed resolution should be based on the center-to-center distance of the double-slit.
Understanding translation as a site of language contact
Authors: Malamatidou Sofia 
Published: September 2016

AbstractThis paper begins by acknowledging translation as an important site of language contact and its primary aim is to reinterpret a theoretical framework from the field of language contact, namely Johanson’s Code-Copying Framework (1993,1999,2002a), with translation in mind. The framework is then systematically applied to empirical data and a corpus-based study is conducted, using the translation of popular science articles from English into Greek as a case in point, and in particular examining any change in the frequency of passive voice reporting verbs. The discussion and corpus analysis suggest that the Code-Copying Framework offers a new vantage point for understanding translation as facilitating linguistic development in the target language, and that translation studies can benefit from adopting it as a descriptive mechanism when comparing instances of contact through translation across languages.
Gains and losses of watching audio described films for sighted viewers
Authors: Perego Elisa 
Published: September 2016

Abstract
Audio description (AD) is a unique form of communication that guarantees access to audiovisual material for blind people through an additional verbal commentary that describes relevant visual cues. In spite of its original purpose and target audience, some scholars and some guidelines maintain that AD can be useful for other sectors of the population, and suggest that sighted viewers could usefully listen to AD when ‘watching’ a film with blind people. In order to determine whether AD affects the sighted viewer’s cognitive performance and appreciation of the film, and whether AD can be exploited as an integration tool allowing blind and sighted users to mingle when ‘watching’ a film, we assessed the effect of AD on sighted viewers (n = 125, 18-28 years) empirically. Results suggest that the addition of AD to films does not negatively affect the cognitive aspects of the viewing experience (i.e., general understanding and film scene recognition), nor does it dramatically affect its overall enjoyment, whereas listening to AD without the visuals poses some challenges to sighted viewers.
The evolution of fan kingdom: the rising, expansion, and challenges of human brands
Authors: Huang Stephen Chi-Tsun , Huang Tsui-Ju 
Published: September 2016

PurposeThe purpose of this paper is to discuss four main research questions which are as follows: how does a consumer turn into a devoted fan? How does a devoted fan react to the expansion of a human brand? What kind of strategies does a devoted fan take when facing challenges encountered by a human brand? And are devoted fans homogeneous, or can they be further divided into different subgroups?Design/methodology/approachThe basis of grounded theory process is intensive depth interviews with 14 devoted fans of a famous Taiwanese pop singer in a qualitative manner along with content analysis of messages from online fan clubs.FindingsUsing the metaphor of kingdom to parallel the phenomenon of fandom, the research also explicates the importance of initial brand position, and the construction and expansion from the core castle – the core positioning of the human brand – to become a kingdom where devoted fans swear to be loyal to the human brand and cross-buy the derivative products of the latter. Five fan’s subgroup and a theoretical framework are obtained.Originality/valueThe theoretical framework derived in this study explicates how consumers’ initial perceptions of the human brand are formed and reinforced and how they become different kinds of fans which in turn influence the strategies they take in the face of the expansion or withdraw of the human brand.