bluuebunny/crossref_metadata_2025_split huggingface dataset:
Controlling protein activity by dynamic recruitment on a supramolecular polymer platform
Authors: Wijnands Sjors P. W. , Engelen Wouter , Lafleur René P. M. , Meijer E. W. , Merkx Maarten 
Published: December 2017

AbstractNature uses dynamic molecular platforms for the recruitment of weakly associating proteins into higher-order assemblies to achieve spatiotemporal control of signal transduction. Nanostructures that emulate this dynamic behavior require features such as plasticity, specificity and reversibility. Here we introduce a synthetic protein recruitment platform that combines the dynamics of supramolecular polymers with the programmability offered by DNA-mediated protein recruitment. Assembly of benzene-1,3,5-tricarboxamide (BTA) derivatives functionalized with a 10-nucleotide receptor strand into µm-long supramolecular BTA polymers is remarkably robust, even with high contents of DNA-functionalized BTA monomers and associated proteins. Specific recruitment of DNA-conjugated proteins on the supramolecular polymer results in a 1000-fold increase in protein complex formation, while at the same time enabling their rapid exchange along the BTA polymer. Our results establish supramolecular BTA polymers as a generic protein recruitment platform and demonstrate how assembly of protein complexes along the supramolecular polymer allows efficient and dynamic control of protein activity.
Evidence of a truncated spectrum in the angular correlation function of the cosmic microwave background
Authors: Melia F. , López-Corredoira M. 
Published: December 2017

Aim. The lack of large-angle correlations in the fluctuations of the cosmic microwave background (CMB) conflicts with predictions of slow-roll inflation. But while probabilities (≲0.24%) for the missing correlations disfavour the conventional picture at ≳3σ, factors not associated with the model itself may be contributing to the tension. Here we aim to show that the absence of large-angle correlations is best explained with the introduction of a non-zero minimum wave number kmin for the fluctuation power spectrum P(k).
Methods. We assumed that quantum fluctuations were generated in the early Universe with a well-defined power spectrum P(k), although with a cut-off kmin ≠ 0. We then re-calculated the angular correlation function of the CMB and compared it with Planck observations.
Results. The Planck 2013 data rule out a zero kmin at a confidence level exceeding 8σ. Whereas purely slow-roll inflation would have stretched all fluctuations beyond the horizon, producing a P(k) with kmin = 0 – and therefore strong correlations at all angles – a kmin ≠ 0 would signal the presence of a maximum wavelength at the time (tdec) of decoupling. This argues against the basic inflationary paradigm, and perhaps even suggests non-inflationary alternatives, for the origin and growth of perturbations in the early Universe. In at least one competing cosmology, the Rh = ct universe, the inferred kmin corresponds to the gravitational radius at tdec.
Traumatic Neonatal Lumbar Punctures: Experience at a Large Pediatric Tertiary Care Center in Canada
Authors: Kollmann Tobias , Matettore Adela 
Published: December 2017

Objective Contamination of cerebrospinal fluid (CSF) by blood during neonatal lumbar puncture (LP) is common and poses diagnostic difficulties. Our objectives were to determine the number of traumatic LPs performed at the BC Children's Hospital over 9 years and whether there was an association between traumatic LPs and demographic variables, hospital location, or time of the procedure.
Study Design This study was a retrospective review of neonatal CSF samples from May 2006 to March 2015. The data were analyzed to establish the rate of traumatic samples and whether there was an association between traumatic LPs and demographic variables (age, gender), location of procedure, positive CSF culture, and/or timing of the procedure.
Results A total of 1,263 LPs were reviewed, 47.7% (n = 602) were contaminated with >400 red blood cells/high-power field. The median age of neonates whose samples were uncontaminated was 10.580 days compared with 6.535 days in the group with contaminated samples (z =  − 2.884, p = 0.004). None of the other factors studied was associated with traumatic taps. Detected organisms included Escherichia coli (n = 12), coagulase-negative Staphylococcus (n = 7), Enterococcus faecalis (n = 3), and group B Streptococcus (n = 2).
Conclusion Nearly half of all CSF samples in the study period were contaminated. Traumatic samples were more common in younger neonates.
Risk of Neonatal Neurologic Morbidity in Advancing Term Gestations
Authors: Stout Molly , Tuuli Methodius , López Julia , Macones George , Cahill Alison , Lewkowitz Adam 
Published: December 2017

Objective Placental insufficiency is associated with neonatal neurologic morbidity and late-term gestations (410/7–416/7 weeks). Whether late-term infants are at increased risk of neurologic morbidity compared with term infants (390/7–406/7 weeks) remains unclear. We aim to compare risk of neurologic morbidity among late-term and term infants.
Study Design This secondary analysis of a single-institution prospective cohort study included all liveborn, nonanomalous singleton term and late-term infants, with data on adverse neonatal outcomes up until 28 days of life. The primary outcome was a neonatal neurologic morbidity composite, defined by having one of these conditions: neonatal seizures, intraventricular hemorrhage, hypoxic-ischemic encephalopathy, and neonatal hypothermic therapy. Secondary outcomes were the composite's individual components and nonneurologic neonatal morbidity. Multivariable logistic regression adjusted for delivery mode, nulliparity, and labor type.
Results Of 5,529 infants included, 747 were late term and 4,782 were term. The risk of composite neurologic morbidity was not significantly different among late-term or term infants (0.5 vs. 0.6%; adjusted odds ratio: 0.59, 95% confidence interval: 0.21–1.71). Overall neonatal morbidity was not significantly different in the two groups, though late-term infants had a nonsignificantly higher prevalence of respiratory distress syndrome (5.5 vs. 3.3%) and meconium aspiration syndrome (0.7 vs. 0.2%).
Conclusion Neonatal neurologic morbidity is uncommon after 39 weeks. Risk does not increase after 41 weeks.
„…dann würde ich traurig werden, weil alle über meinen Papa reden“ – Die Rolle des Stigmas in Familien mit psychisch erkranktem Elternteil
Authors: Wahl Patricia , Otto Christina , Lenz Albert 
Published: December 2017

ZusammenfassungDie Stigmatisierung psychischer Erkrankungen ist ein gesellschaftliches Problem, das auch für die Hilfesuche eine Rolle spielt. Im Rahmen einer qualitativen Interviewstudie wurde eruiert, welche Rolle Stigma bei der Hilfesuche aus Sicht von psychisch erkrankten Eltern und ihren Angehörigen spielt. Auch die Perspektive ihrer Kinder wurde einbezogen. Erkrankte Eltern räumten dem Thema Stigmatisierung eine wichtige Rolle im Zusammenhang mit Hilfesuchprozessen ein, die Kinder machten hierzu kaum explizite Äußerungen, ein implizites Bewusstsein für Stigmatisierung scheint aber vorhanden zu sein.
Phosphorus-bearing molecules in the Galactic Center
Authors: Rivilla V M , Jiménez-Serra I , Zeng S , Martín S , Martín-Pintado J , Armijos-Abendaño J , Viti S , Aladro R , Riquelme D , Requena-Torres M , Quénard D , Fontani F , Beltrán M T 
Published: December 2017

Abstract
Phosphorus (P) is one of the essential elements for life due to its central role in biochemical processes. Recent searches have shown that P-bearing molecules (in particular PN and PO) are present in star-forming regions, although their formation routes remain poorly understood. In this letter, we report observations of PN and PO towards seven molecular clouds located in the Galactic Center, which are characterized by different types of chemistry. PN is detected in five out of seven sources, whose chemistry is thought to be shock-dominated. The two sources with PN non-detections correspond to clouds exposed to intense UV/X-rays/cosmic ray (CR) radiation. PO is detected only towards the cloud G+0.693−0.03, with a PO/PN abundance ratio of ∼1.5. We conclude that P-bearing molecules likely form in shocked gas as a result of dust grain sputtering, while are destroyed by intense UV/X-ray/CR radiation.
Update of a Systematic Review of Autoresuscitation After Cardiac Arrest
Authors: Hornby Laura , Dhanani Sonny , Shemie Sam D. 
Published: December 2017

Objectives:
There has been a growth in publications focusing on the phenomena of autoresuscitation in recent years. In 2010, we systematically reviewed the medical literature with the primary objective of summarizing the evidence on the timing of autoresuscitation. Healthcare professionals have continued to voice concerns regarding the potential for autoresuscitation. With this in mind, the objective of this brief report is to update the results of our original review of autoresuscitation.

Data Sources:
We applied the same search strategy described in our original article to update our findings to include articles published from January 2009 to September 2016.

Study Selection and Data Extraction:
We screened an additional 1,859 citations, after duplicates were removed, and then assessed 46 full-text articles for eligibility, from which 15 studies were included for data extraction.

Data Synthesis:
During the time period of this review, there have been 1) 10 additional adult and three pediatric case reports of autoresuscitation in patients after cessation of cardiopulmonary resuscitation; in those cases with continuous monitoring and confirmation of circulation, the longest events are reported to be 10 and 2 minutes, respectively for adults and children; 2) six adults (4%, total n = 162) with autoresuscitation events reported from two observational studies and one chart review of patients undergoing withdrawal of life-sustaining therapy; the longest time reported to be 89 seconds with electrocardiogram and invasive arterial blood pressure monitoring and 3 minutes with electrocardiogram monitoring only; 3) 12 pediatric patients studied with vital sign monitoring during withdrawal of life-sustaining therapy without any reports of autoresuscitation.

Conclusions:
Although case reports of autoresuscitation are hampered by variability in observation and monitoring techniques, autoresuscitation has now been reported in adults and children, and there appears to be a distinction in timing between failed cardiopulmonary resuscitation and withdrawal of life-sustaining therapy. Although additional prospective studies are required to clarify the frequency and predisposing factors associated with this phenomenon, clinical decision-making regarding patient management under uncertainty is required nonetheless. Both adult and pediatric healthcare professionals should be aware of the possibility of autoresuscitation and monitor their patients accordingly before diagnosing death.
Split Tolerance in a Murine Model of Heterotopic En Bloc Chest Wall Transplantation
Authors: Oh Byoungchol , Furtmüller Georg J. , Malek Veronika , Fryer Madeline L. , Brayton Cory , Walczak Piotr , Janowski Miroslaw , Brandacher Gerald , Dorafshar Amir H. 
Published: December 2017

Background:
Congenital and acquired chest wall deformities represent a significant challenge to functional reconstruction and may impact feasibility of heart transplantation for patients with end-stage organ failure. In the recent past, the concept of replacing like-with-like tissue by using vascularized composite allografts (VCA) has been enthusiastically employed for reconstruction of complex tissue defects.

Methods:
In this study, we introduce a novel murine model for en bloc chest wall, heart, and thymus transplantation and thereby the use of complex tissue allografts for reconstruction of both chest wall defects and also end-stage organ failure. Additionally, this model allows us to study the features of combined vascularized bone marrow (VBM), thymus, and heart transplantation on allograft survival and function. Heterotopic chest wall, thymus, and heart transplants were performed in untreated syngeneic and allogeneic combinations and in allogeneic combinations treated with costimulation blockade (CTLA4-Ig and MR-1).

Results:
Indefinite (ie, 150 d, N = 3) graft survival was observed in syngeneic controls. In untreated recipients of allogeneic grafts, the skin component was rejected after 10 (±1) days, whereas rejection of the heart occurred after 13 (± 1) days (N = 3). Costimulation blockade treatment prolonged survival of the heart and chest wall component (130 d, N = 3) as well as the VBM niche as evidenced by donor-specific chimerism (average: 2.35 ± 1.44%), whereas interestingly, the skin component was rejected after 13 (±1) days.

Conclusion:
Thus, this novel microsurgical model of VCA combined with solid organ transplantation is technically feasible and results in split tolerance when treated with costimulatory blockade.
Early Markers of Sickle Nephropathy in Children With Sickle Cell Anemia Are Associated With Red Cell Cation Transport Activity
Authors: Brewin John , Tewari Sanjay , Hannemann Anke , Al Balushi Halima , Sharpe Claire , Gibson John S. , Rees David C. 
Published: December 2017

AbstractThe early stages of sickle cell nephropathy (SCN) manifest in children with sickle cell anemia (SCA) as hyperfiltration and proteinuria. The physiological conditions of the renovascular system are among the most conducive to hemoglobin S polymerization in the body and will magnify small changes in red cell volume thus crucially modulating intracellular concentrations of hemoglobin S. This large cross‐sectional study of children with sickle cell anemia measured glomerular filtration rates and microalbuminuria to report prevalence, clinical correlates and uniquely, association with key red cell cation transport mechanisms. One hundred and twelve patients (mean age 10.7 ± 4.1) were recruited. The prevalence of hyperfiltration and microalbuminuria was 98% and 15.1%, respectively. Glomerular filtration rates did not vary with age, but proteinuria became more prevalent with increasing age. Both features associated with markers of hemolysis, while elevated hemoglobin F was protective, but no association was seen with systolic or diastolic blood pressure. In multivariate analysis, both Gardos channel (β = 0.476, P < 0.001) and KCl co‐transporter (KCC; β = −0.216, P = 0.009) activity, alongside age (β = 0.237, P = 0.004), remained independently predictive for microalbuminuria. Increased activity of Gardos channel and Psickle positively associated with microalbuminuria, while increased KCC activity associated with a reduction in microalbuminuria. This study demonstrates a direct link between the abnormally active red cell cation transport systems in sickle cell disease and sickle organopathy. Small variations in the activity of these transport mechanisms predict for SCN and measurement of them may help identify those at risk, while pharmaceutical manipulation of these excessively active systems may ameliorate their risk.
Reduced‐Intensity Chemotherapy in Patients With Advanced‐Stage Hodgkin Lymphoma
Authors: Engert Andreas , Goergen Helen , Markova Jana , Pabst Thomas , Meissner Julia , Zijlstra Josée M. , Král Zdenek , Eichenauer Dennis A. , Soekler Martin , Greil Richard , Kreissl Stefanie , Scheuvens Ruth , Eich Hans , Kobe Carsten , Dietlein Markus , Stein Harald , Fuchs Michael , Diehl Volker , Borchmann Peter 
Published: December 2017

AbstractThe international, randomized phase 3 HD15 trial established 6xeBEACOPP as standard therapy for patients with newly diagnosed advanced‐stage Hodgkin lymphoma (HL) within the German Hodgkin Study Group (GHSG). We performed a follow‐up analysis to assess long‐term efficacy and safety of this approach. Between 2003 and 2008, 2182 patients aged 18 to 60 years were recruited and randomized in a 1:1:1 ratio between 8 or 6 cycles of eBEACOPP or 8 cycles of the dose‐dense BEACOPP‐14 regimen, each followed by 30 Gy radiotherapy in case of positron emission tomography (PET)‐positive residual lesions ≥2.5 cm. The study aimed at demonstrating non‐inferiority regarding efficacy of the 2 experimental arms on a significance level of 2.5% each. The intention‐to‐treat analysis comprised 2126 patients with a median follow‐up of 102 months. Ten‐year progression‐free survival was 81% (97.5% CI 77–85) with 8xeBEACOPP, 84% (80–87) with 6xeBEACOPP, and 84% (80–87) with 8xBEACOPP‐14; the non‐inferiority margin of 1.51 for the hazard ratio (HR) could be excluded for both comparisons (6xeBEACOPP, HR = 0.7, 97.5% CI 0.5–1.0; 8xBEACOPP‐14, HR = 0.9, 97.5% CI 0.7–1.2). Overall survival at 10 years was 88% (85–91), 90% (88–93), and 92% (89–94), respectively. A total of 142 second malignancies corresponding to 10‐year cumulative incidences of 10%, 7%, and 7% and standardized incidence ratios of 4.3, 2.5, and 2.8 were reported for 8xeBEACOPP, 6xeBEACOPP, and 8xBEACOPP‐14, respectively. This updated analysis of the HD15 trial thus confirms the efficacy and reports on the long‐term safety of a shortened first‐line chemotherapy consisting of 6xeBEACOPP followed by PET‐guided radiotherapy in advanced‐stage HL.
Linking the Essential Core Competencies to Pediatric Content in an Entry-Level Physical Therapy Education Curriculum: A Case Report
Authors: Cech Donna , Krzak Joseph J. , Anderson Deborah K. 
Published: December 2017

Background and Purpose.
In 2012, the Section on Pediatrics (SoP) established five Essential Core Competencies in Entry-Level Pediatric Physical Therapy Education representing the expected pediatric knowledge base, skills, and abilities of all graduating physical therapist (PT) students. To facilitate the integration of the Core Competencies within curricula and to achieve intended academic objectives, educators need tools to connect or “link” specific didactic content to each of the five Core Competencies and their associated subcriteria. The purpose of the current case report was to introduce a novel systematic approach using linking rules to demonstrate comprehensive inclusion of Essential Core Competencies within professional (entry-level) PT education programs.

Case Description.
Course descriptions and objectives within the curriculum of an accredited entry-level physical therapy professional program were reviewed for pediatric content. Two investigators independently assigned pediatric curriculum content to the 5 Essential Core Competencies using 10 linking rules. A third investigator was used to establish a consensus during the linking process. Frequencies and percentages representing the inclusion of each of the competencies and their associated subcriteria were calculated. Percent agreement and Cohen's kappa (κ) between the two independent investigators were calculated to evaluate the interrater reliability of the linking process.

Outcomes.
One hundred sixty-five (17%) of the 976 course objectives within the entry-level physical therapy professional program contained pediatric content. All 5 of the Essential Core Competencies were represented at varying degrees: human development (17%), age-appropriate patient/client management (51%), family-centered care for all patient/client and family interactions (6%), health promotion and safety (12%), and legislation, policy, and systems (7%). The remaining objectives (7%) were considered either General Pediatric Content or Not Covered by the Core Competencies, or Not Definable. The linking process had substantial interrater reliability (κ = 0.76; standard error = 0.09; 95% confidence interval = 0.60–0.93) between the two independent investigators.

Discussion and Conclusion.
A systematic approach to linking pediatric curriculum content to the Essential Core Competencies was introduced and applied using an integrated professional (entry-level) physical therapy education program. Such reliable systematic processes support best educational practice by confirming that all relevant pediatric content is preserved throughout an integrated physical therapy curriculum and ensures that PT students are provided with the essential knowledge base, skills, and abilities needed to successfully work with children. Future research should apply the linking strategies to nonintegrated physical therapy curricula, programs in other geographic regions, and content outside of pediatrics.
Activities of the THOR-AABB Working Party
Authors: Yazer Mark H. , Spinella Philip C. 
Published: December 2017

ABSTRACT
The AABB (formerly the American Association of Blood Banks) is an international authority on transfusion medicine and tissue banking. The Trauma, Hemostasis and Oxygenation Research (THOR) Network is an international multidisciplinary network of civilian and military providers ranging from first responders and medics to critical care physicians and from basic scientists to clinical trialists. The THOR Network's vision is to improve outcomes from traumatic hemorrhagic shock by optimizing the acute phase of resuscitation. Its mission is to develop and implement best practices for prehospital care through to the completion of the acute phase of hemorrhagic shock resuscitation. Thus, there is significant overlap between the missions of these two groups. To this end, the joint THOR-AABB Working Party (WP) was created in the summer of 2016 with a view to improving patient outcomes by the establishment of a formal collaboration between these two groups. The WP has been engaged in many different endeavors, from successfully changing the AABB's standards for the administration of whole blood, to writing commentaries on the safety of unncrossmatched red blood cells and antibody titer methods and thresholds in potentially incompatible plasma products, to hosting a daylong symposium on blood product resuscitation of massively bleeding patients in conjunction with the AABB annual meeting. This review details the activities of the WP and indicates some future activities.