bluuebunny/crossref_metadata_2025_split huggingface dataset:
Injury mechanisms and electromyographic changes after injury of the recurrent laryngeal nerve: Experiments in a porcine model
Authors: Brauckhoff Katrin , Svendsen Øyvind S. , Stangeland Lodve , Biermann Martin , Aas Turid , Husby Paul J. A. 
Published: September 2017

AbstractBackgroundRecurrent laryngeal nerve (RLN) injury during surgery may reveal differences in electromyographic (EMG) changes after sustained compression or traction.MethodsIn 20 pigs with the NIM‐FLEX EMG‐endotracheal tube, EMG was recorded at baseline, during sustained RLN compression, or traction until 70% amplitude decrease and during 30 minutes of recovery.ResultsSeventy percent amplitude decrease from baseline was reached after 110 ± 98 seconds (compression group) and 2034 ± 2108 seconds (traction group). Traction induced a pronounced latency increase, peaking at 122 ± 8% in contrast to compression with 106 ± 5% (P < .001). The EMG amplitude recovery to ≥50% of baseline failed in 7 nerves after compression and 8 nerves after traction.ConclusionCompression caused a fast decrease of EMG amplitude with minor effects on latency. In contrast, RLN traction showed early and significant latency increase preceding a delayed amplitude decrease. Recovery rate of the EMG signals were similar in both groups.
Genomic profiling of intestinal‐type sinonasal adenocarcinoma reveals subgroups of patients with distinct clinical outcomes
Authors: López‐Hernández Alejandro , Pérez‐Escuredo Jhudit , Vivanco Blanca , García‐Inclán Cristina , Potes‐Ares Sira , Cabal Virginia N. , Riobello Cristina , Costales María , López Fernando , Llorente José Luis , Hermsen Mario A. 
Published: September 2017

AbstractBackgroundPatients with intestinal‐type sinonasal adenocarcinoma (ITAC) have an unfavorable prognosis and new therapeutic approaches are needed to improve clinical management.MethodsGenetic analysis of 96 ITACs was performed by microarray comparative genomic hybridization and immunohistochemistry and correlated to previously obtained mutation, methylation, and protein expression data, and with pathological characteristics and clinical outcome.ResultsSeven copy number alterations (CNAs) were significantly associated with unfavorable clinical outcome: gains at 1q22‐23, 3q28‐29, 6p22, and 13q31‐33, and losses at 4p15‐16, 4q32‐35, and 10q24. Unsupervised cluster analysis resulted in 5 subgroups of ITAC with significantly distinct genetic signatures and clinical outcomes, independently of disease stage or histological subtype.ConclusionThese data may guide studies to identify driver genes and signaling pathways involved in ITAC. In addition, the subclassification of genetic subgroups of patients with distinct clinical behavior can aid therapeutic decision making and may ultimately lead to personalized therapy with targeted inhibitors.
Comparison of outcomes with extensive segmental pectoralis major myocutaneous flap via the anterior axillary line and the conventional technique in oral and oropharyngeal cancer
Authors: Chen Wei‐liang , Zhang Da‐ming , Huang Zhi‐quan , Wang Yan , Zhou Bin , Wang You‐yuan 
Published: September 2017

AbstractBackgroundThis study compared the outcomes of an extensive segmental pectoralis major myocutaneous flap (esPMMF) and a conventional pectoralis major myocutaneous flap (PMMF).MethodsThe study enrolled 91 patients with primary oral and oropharyngeal squamous cell carcinoma (SCC) who underwent radical resection followed by reconstruction of the defect using either an esPMMF via the anterior axillary line or a PMMF. The pedicle lengths of the esPMMF and PMMF were 22‐28 and 18‐22 cm, respectively. The esPMMF and PMMF had skin paddle dimensions of 5 × 8 to 7 × 14 cm and 6 × 7 to 8 × 17 cm, respectively.ResultsThe esPMMF pedicle was longer than that of the PMMF. The range of shoulder abduction was significantly greater in the esPMMF group and the donor‐site aesthetic results were better.ConclusionThe esPMMF has a longer pedicle flap, enables a greater range of shoulder abduction, and has a better aesthetic result than the conventional technique.
Predictors of contralateral‐bilateral nodal disease in oropharyngeal cancer: A National Cancer Data Base Study
Authors: Kato Masanari G. , Ellis Mark A. , Nguyen Shaun A. , Day Terry A. 
Published: September 2017

AbstractBackgroundOropharyngeal squamous cell carcinoma (SCC) frequently presents with cervical metastasis. Roles of human papillomavirus (HPV) status, among other factors, on laterality are elusive.MethodsThe National Cancer Database was reviewed for oropharyngeal SCC diagnosed from 2010‐2014. Predictors of clinically evident contralateral or bilateral nodal disease were identified.ResultsA total of 15 517 patients with oropharyngeal SCC met criteria. The majority was HPV‐positive. Histologically poorly differentiated tumors were more frequent in the HPV‐positive group (55.7% vs 37.6%; P < .001). By incidence, there was no statistical difference in contralateral or bilateral nodal disease between HPV‐positive and HPV‐negative patients (14.2% vs 14.5%, respectively; P = .769). On multivariable analysis, notable predictors of contralateral or bilateral nodal disease included HPV‐positivity (odds ratio [OR] 1.26; 99% confidence interval [CI] 1.10‐1.44), base of tongue (BOT) location (OR 2.15; 99% CI 1.88‐2.45), poorly differentiated tumor (OR 1.72; 99% CI 1.20‐2.46), and T4 classification (OR 6.65; 99% CI 5.34‐8.28).ConclusionPatients with HPV‐associated oropharyngeal SCC have increased likelihood of contralateral or bilateral nodal disease. Tumor grade, tumor location, and tumor size are also independent predictors.
Endometrial receptivity and conception outcome among women with light menstrual bleeding of unidentified etiology
Authors: Gao Yueqing , Hong Xiangli , Wang Zhewei , Zhu Ying 
Published: September 2017

AbstractObjectiveTo investigate endometrial receptivity (ER), conception outcome, and other factors among women with light menstrual bleeding of unidentified etiology.MethodsA prospective study was undertaken at a center in China. Between January 1, 2011, and December 31, 2014, women meeting the inclusion criteria (aged 20–35 years, without any history of pregnancy, desire to conceive, follicle‐stimulating hormone <10 IU on second or third day of cycle, and regular menstrual periods) were enrolled. Participants with a menstrual blood volume of 5–20 mL formed the study group; others were in the control group. ER measures were obtained by transvaginal Doppler ultrasonography. Serum estradiol and progesterone levels were measured and items of prethrombotic state were screened in mid‐luteal phase. Participants were followed up for 1 year to establish conception outcome.ResultsThe ER (Salle) score was significantly lower in the study group (n=110) than in the control group (n=330; 9.41 ± 3.02 vs 15.67 ± 2.63, P<0.001). The natural rate of conception within 1 year was lower (60.0% [63/105] vs 72.1% [230/319], P=0.020), and the spontaneous abortion rate in early pregnancy was higher (34.4% [21/61] vs 18.1% [41/227], P=0.006) in the study group than in the control group.ConclusionWomen with light menstrual bleeding of unidentified etiology had an increased prevalence of low ER and poor conception outcome.
Hemocompatibility of hyaluronan enhanced linear low density polyethylene for blood contacting applications
Authors: Simon‐Walker Rachael , Cavicchia John , Prawel David A. , Dasi Lakshmi Prasad , James Susan P. , Popat Ketul C. 
Published: September 2017

AbstractDespite their overall success, different blood‐contacting medical devices such as heart valves, stents, and so forth, are still plagued with hemocompatibility issues which often result in the need for subsequent replacement and/or life‐long anticoagulation therapy. Consequently, there is a significant interest in developing biomaterials that can address these issues. Polymeric‐based materials have been proposed for use in many applications due to their ability to be finely tuned through manufacturing and surface modification to enhance hemocompatibility. In this study, we have developed a novel, hydrophilic biomaterial comprised of an interpenetrating polymer network (IPN) of hyaluronan (HA) and linear low density polyethylene (LLDPE). HA is a highly lubricous, anionic polysaccharide ubiquitously found in the human body. It is currently being investigated for a vast array of biomedical applications including cardiovascular therapies such as hydrogel‐based regenerative cell therapies for myocardial infarction, HA‐coated stents, and surface modifications of polyurethane and metals for use in blood‐contacting implants. The aim of this study was to assess the in vitro thrombogenic response of the hydrophilic polymer surface, HA‐LLDPE for future potential use as flexible heart valve leaflets. The results indicate that HA‐LLDPE is non‐toxic and reduces thromobogenicity as compared to LLDPE surfaces, asserting its feasibility for use as a blood‐contacting biomaterial. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1964–1975, 2018.
Comparison of Cyanex 272 and Cyanex 572 for the separation of Neodymium from a Nd/Tb/Dy mixture by pertraction
Authors: Pavon Sandra , Kutucu Merve , Coll M Teresa , Fortuny Agustin , Sastre Ana M 
Published: September 2017

AbstractBACKGROUNDRecovering Nd(III) from waste magnets is an alternative method to satisfy the increasing demand for this metal. For this reason, the separation of Nd from a mixture containing Nd/Tb/Dy in chloride media using Cyanex 272 and Cyanex 572 has been evaluated.RESULTSUsing Cyanex 272 and Cyanex 572, the metals are transported in the order Dy(III) > Tb(III) > > Nd(III) in all conditions studied. The optimum feed conditions to achieve Nd(III) separation are: Cyanex 272: pH 2 and Cyanex 572: pH 1.5 with 1.2 mol L‐1 HCl as a receiving agent for both carriers.CONCLUSIONThe results obtained suggest that Cyanex 572 is a better carrier than Cyanex 272 for separating Nd(III) from a mixture containing Nd/Tb/Dy. © 2017 Society of Chemical Industry
Polyphenols extracted from red grape pomace by a surfactant based method show enhanced collagenase and elastase inhibitory activity
Authors: Mohd Maidin Nurmahani , Michael Nicholas , Oruna‐Concha Maria Jose , Jauregi Paula 
Published: September 2017

AbstractBACKGROUNDThe aim of this study is to separate polyphenols from grape pomace using a surfactant‐based separation, colloidal gas aphrons (CGA) and to investigate their inhibitory activity against skin relevant enzymes, collagenase and elastase. Ethanolic (EE) and hot water crude extracts (HWE) were produced first and then the CGA generated using TWEEN20 were applied resulting in polyphenols enriched fractions (CGA‐EE and CGA‐HWE, ethanol and hot water extracts derived fractions, respectively).RESULTSBoth crude extracts inhibited the enzymes in a dose‐dependent manner, however, further extraction by CGA led to fractions with higher inhibitory efficiency against collagenase. Although gallic acid was the main component of the CGA‐HWE, others such as kaempferol must have contributed to its potency which was more than six times that of gallic acid. The CGA‐EE was found to be about four times more efficient than its crude extract and more than six times more efficient than gallic acid for collagenase inhibition; quercetin was the major polyphenol in this fraction.CONCLUSIONIt is evident that ethanol and hot water extraction processes led to different polyphenols composition and thus different inhibitory activity against collagenase and elastase. Further separation with CGA increased the inhibitory potency of both extracts against collagenase. Overall the results here showed the potential for application of CGA fractions from grape extracts in cosmetics. © 2017 Society of Chemical Industry
LYTAG‐driven purification strategies for monoclonal antibodies using quaternary amine ligands as affinity matrices
Authors: Campos‐Pinto Isabel , Capela Emanuel V , Silva‐Santos A Rita , Rodríguez Miguel Arévalo , Gavara Poondi Rajesh , Fernandez‐Lahore Marcelo , Aires‐Barros M Raquel , Azevedo Ana M 
Published: September 2017

AbstractBACKGROUNDMonoclonal antibodies are becoming a leading class of biopharmaceuticals but to increase their accessibility by the general population, new production processes must be developed in particular for the downstream processing.RESULTSIn this work, an alternative and innovative affinity chromatographic method using quaternary amine matrices is proposed. Separation is driven by the dual affinity ligand LYTAG‐Z, composed of a choline binding polypeptide (LYTAG) and the synthetic antibody binding Z domain. A two‐elution method was developed for the purification of mAbs and the performance of different anion exchangers containing quaternary amines that act as choline analogues – CIMmultus Q, Q Sepharose and gPore Q – were tested and compared, with both CIMmultus Q and Q Sepharose allowing a recovery of more than 94% of mAbs from a CHO cell supernatant with a purity greater than 95%.An integrated platform combining an initial affinity extraction step for the clarification and capture of mAbs and a subsequent chromatographic separation using Q‐matrices for the polishing of mAbs is also proposed. LYTAG‐Z triggers the extraction of 94.7 ± 1.7% mAbs to the PEG‐rich phase, as opposed to 26.9 ± 0.6% in the absence of the ligand, using 7% PEG 3350 and 6% dextran 500 k. Further purification using Q Sepharose allowed a mAb recovery of 95.3 ± 1.4% with a purity level of 91.4 ± 13.0%.CONCLUSIONAn integrated platform based on two purification steps – affinity extraction and affinity chromatography – results in an overall process yield of 90%, allowing the processing of mAbs directly from a non‐clarified CHO cell culture. © 2017 Society of Chemical Industry
Application of autofluorescence robotic histology for quantitative evaluation of the 3‐dimensional morphology of murine articular cartilage
Authors: Das Neves Borges Patricia , Vincent Tonia L. , Marenzana Massimo 
Published: September 2017

AbstractMurine models of osteoarthritis (OA) are increasingly important for understating pathogenesis and for testing new therapeutic approaches. Their translational potential is, however, limited by the reduced size of mouse limbs which requires a much higher resolution to evaluate their articular cartilage compared to clinical imaging tools. In experimental models, this tissue has been predominantly assessed by time‐consuming histopathology using standardized semi‐quantitative scoring systems. This study aimed to develop a novel imaging method for 3‐dimensional (3D) histology of mouse articular cartilage, using a robotic system—termed here “3D histocutter”—which automatically sections tissue samples and serially acquires fluorescence microscopy images of each section. Tibiae dissected from C57Bl/6 mice, either naïve or OA‐induced by surgical destabilization of the medial meniscus (DMM), were imaged using the 3D histocutter by exploiting tissue autofluorescence. Accuracy of 3D imaging was validated by ex vivo contrast‐enhanced micro‐CT and sensitivity to lesion detection compared with conventional histology. Reconstructions of tibiae obtained from 3D histocutter serial sections showed an excellent agreement with contrast‐enhanced micro‐CT reconstructions. Furthermore, osteoarthritic features, including articular cartilage loss and osteophytes, were also visualized. An in‐house developed software allowed to automatically evaluate articular cartilage morphology, eliminating the subjectivity associated to semi‐quantitative scoring and considerably increasing analysis throughput. The novelty of this methodology is, not only the increased throughput in imaging and evaluating mouse articular cartilage morphology starting from conventionally embedded samples, but also the ability to add the third dimension to conventional histomorphometry which might be useful to improve disease assessment in the model.
Advanced and amplified BOLD fluctuations in high‐grade gliomas
Authors: Gupta Lalit , Gupta Rakesh K. , Postma Alida A. , Sahoo Prativa , Gupta Pradeep K. , Patir Rana , Ahlawat Sunita , Saha Indrajit , Backes Walter H. 
Published: September 2017

BackgroundGlioma grade along with patient's age and general health are used for treatment planning and prognosis.PURPOSETo characterize and quantify the spontaneous blood oxygen level‐dependent (BOLD) fluctuations in gliomas using measures based on
‐weighted signal time‐series and to distinguish between high‐ and low‐grade gliomas.Study TypeRetrospective.SubjectsTwenty‐one patients with high‐grade and 13 patients with low‐grade gliomas confirmed on histology were investigated.Field Strength/SequenceDynamic
‐weighted (multislice single‐shot echo‐planar‐imaging) magnetic resonance imaging (MRI) was performed on a 3T system with an 8‐element receive‐only head coil to measure the BOLD fluctuations. In addition, a dynamic T1‐weighted (3D fast field echo) dynamic contrast‐enhanced (DCE) perfusion scan was performed.AssessmentThree BOLD measures were determined: the temporal shift (TS), amplitude of low frequency fluctuations (ALFF), and regional homogeneity (ReHo). DCE perfusion‐based cerebral blood volume (CBV) and time‐to‐peak (TTP) maps were concurrently evaluated for comparison.Statistical TestsAn analysis‐of‐variance test was first used. When the test appeared significant, post‐hoc analysis was performed using analysis‐of‐covariance with age as covariate. Logistic regression and receiver‐operator characteristic curve analysis were also performed.ResultsTS was significantly advanced in high‐grade gliomas compared to the contralateral cortex (P = 0.01) and low‐grade gliomas (P = 0.009). In high‐grade gliomas, ALFF and CBV were significantly higher than the contralateral cortex (P = 0.041 and P = 0.008, respectively) and low‐grade gliomas (P = 0.036 and P = 0.01, respectively). ReHo and TTP did not show significant differences between high‐ and low‐grade gliomas (P = 0.46 and P = 0.42, respectively). The area‐under‐curve was above 0.7 only for the TS, ALFF, and CBV measures.Data ConclusionAdvanced and amplified hemodynamic fluctuations manifest in high‐grade gliomas, but not in low‐grade gliomas, and can be assessed using BOLD measures. Preliminary results showed that quantification of spontaneous fluctuations has potential for hemodynamic characterization of gliomas and distinguishing between high‐ and low‐grade gliomas.Level of Evidence: 4Technical Efficacy: Stage 5J. Magn. Reson. Imaging 2018;47:1616–1625.
Tritordeum: a novel cereal for food processing with good acceptability and significant reduction in gluten immunogenic peptides in comparison with wheat
Authors: Vaquero Luis , Comino Isabel , Vivas Santiago , Rodríguez‐Martín Laura , Giménez María J , Pastor Jorge , Sousa Carolina , Barro Francisco 
Published: September 2017

AbstractBACKGROUNDTritordeum is a novel cereal obtained from the hybridization between durum wheat and a wild barley. This study evaluates acceptance, digestibility and immunotoxic properties of tritordeum, a novel cereal for food processing. Nineteen healthy volunteers participated in a study with different diets to compare tritordeum bread with wheat and gluten‐free breads.RESULTSTritordeum breads had a similar acceptance to the wheat bread usually consumed, and the acceptance was significantly higher than the gluten‐free bread and standardized wheat bread supplied in the study. There was no evidence for gastrointestinal symptoms among volunteers during the study. The reductions in the numbers of immunogenic epitopes in tritordeum in comparison with wheat were 78% for α‐gliadins, 57% for γ‐gliadins and 93% for ω‐gliadins. The analysis of gluten immunogenic peptides (GIP) in stool samples showed a significantly lower excretion in the tritordeum ingestion phase than in the wheat ingestion phase.CONCLUSIONSThese results suggest that tritordeum may be an option of interest for general food processing, and especially for those who want to reduce their intake of gluten. However, it is not suitable for celiac disease sufferers as it contains gluten. © 2017 Society of Chemical Industry
Valorization of underutilized North American pawpaw (Asimina triloba): investigation as a lipid oxidation inhibitor in turkey homogenate model system
Authors: Brannan Robert G , Peters Trisha E , Black Kathleen J , Kukor Benjamin J 
Published: September 2017

AbstractBACKGROUNDThe objective of this study was to characterize the ability of extracts from nine varieties of pawpaw pulp standardized to the phenolics level of 0.1% grape seed extract (GSE) on inhibition of the formation of thiobarbituric reactive substances (TBARS) in a turkey model system. The antioxidant activity of the extracts was also determined using four common assays.RESULTSOver the 240 min sampling time, the standardized pawpaw extracts from all nine varieties were as effective as GSE in inhibiting TBARS formation in turkey muscle homogenate compared to the untreated control. Extracts from all pawpaw varieties and GSE began to inhibit TBARS formation at 60 min of incubation, and by 240 min TBARS were reduced from 35 μmol malondialdehyde kg−1 tissue in the homogenate to which no antioxidant was added to 4–18 μmol malondialdehyde kg−1 tissue in the antioxidant‐enriched extracts. There does not appear to be a clear relationship between inhibition of TBARS and any of the antioxidant capacity measurements (ORAC, DPPH inhibition, reducing potential as measured by FRAP assay, or pyrogallol red bleaching).CONCLUSIONThe results of this research indicate that there is potential to add value to pawpaw as a functional food source of natural antioxidants, particularly in meat products. © 2017 Society of Chemical Industry
Traceability of Boletaceae mushrooms using data fusion of UV–visible and FTIR combined with chemometrics methods
Authors: Yao Sen , Li Tao , Liu HongGao , Li JieQing , Wang YuanZhong 
Published: September 2017

AbstractBACKGROUNDBoletaceae mushrooms are wild‐grown edible mushrooms that have high nutrition, delicious flavor and large economic value distributing in Yunnan Province, China. Traceability is important for the authentication and quality assessment of Boletaceae mushrooms. In this study, UV–visible and Fourier transform infrared (FTIR) spectroscopies were applied for traceability of 247 Boletaceae mushroom samples in combination with chemometrics.RESULTSCompared with a single spectroscopy technique, data fusion strategy can obviously improve the classification performance in partial least square discriminant analysis (PLS‐DA) and grid‐search support vector machine (GS‐SVM) models, for both species and geographical origin traceability. In addition, PLS‐DA and GS‐SVM models can provide 100.00% accuracy for species traceability and have reliable evaluation parameters. For geographical origin traceability, the accuracy of prediction in the PLS‐DA model by data fusion was just 64.63%, but the GS‐SVM model based on data fusion was 100.00%.CONCLUSIONThe results demonstrated that the data fusion strategy of UV–visible and FTIR combined with GS‐SVM could provide a higher synergic effect for traceability of Boletaceae mushrooms and have a good generalization ability for the comprehensive quality control and evaluation of similar foods. © 2017 Society of Chemical Industry