bluuebunny/crossref_metadata_2025_split huggingface dataset:
Future workplace reimagination: A multiscenario analysis on entrepreneurial small and medium internationalisers
Authors: Mahdiraji Hannan Amoozad , Yaftiyan Fatemeh , Sadeghi Vahid J. , Vrontis Demetris 
Published: September 2024

AbstractThis paper aims to encounter the scholarly demand for comprehensive identification and investigation of the factors that highlight the sense of the “workplace of the future.” Besides, this study sheds in‐depth qualitative and quantitative insights into analysing such drivers in international entrepreneurial small and medium enterprises of emerging economies like Iran. A mixed method of systematic literature review and hesitant triangular fuzzy Delphi was initially employed to identify and finalise the significant factors. Afterwards, the causal level‐based relationship network and the finalised factors' power map were accomplished for three distinct scenarios (i.e., the pessimistic, probable and optimistic conditions). To this end, a novel integration of hesitant triangular fuzzy sets and interpretive structural modelling‐matrix‐based multiplication applied to a classification was designed and applied. As a result, future workplace concerns were systematically reviewed. From the initial list of 34 factors, eight were selected from the perspective of three panels of experts (15 international entrepreneurs) as the most crucial for international small and medium enterprises due to implementing the hesitant triangular fuzzy sets. Furthermore, the selected factors were categorised and levelled according to the novel interpretive structural modelling‐matrix‐based multiplication applied to a classification method developed in this article. Accordingly, for each scenario studied, from low to high uncertainty and indeterminacy, a separate conceptual model illustrating the relationship among the factors propelling the reimagining of the future of the workplace was presented.
The repertoire of G‐protein‐coupled receptor variations in the Japanese population 54KJPN
Authors: Ikuta Tatsuya , Suzuki Riko , Inoue Asuka 
Published: September 2024

AbstractG‐protein‐coupled receptors (GPCRs) are the largest superfamily in the human genome and the major targets for the market drugs. Recent massive genomics studies revealed numerous natural variations in the general population. 54KJPN is the most extensive Japanese population genomics study, curating the whole genome sequences from about 54,000 individuals. Here, by analyzing 390 non‐olfactory GPCR genes in the 54KJPN dataset, we annotated 25,443 missense single‐nucleotide variations. Among them, we found 120 major variations that appear with an allele frequency greater than 0.5, including variations that occurred on posttranslational modification sites. Structural alignment of GPCRs using the generic numbering system in the GPCRdb reveals enrichment of alterations in the conserved arginine residue within the DRY motif, which contributes to downstream G‐protein signaling. A comparison with the worldwide 1000 Genomes Project (1KGP) dataset found 23 variations that were present exclusively in the 54KJPN dataset. This study will be the basis for future pharmacogenomics studies for the Japanese population.
Exploring Consumer Experiences of Barriers and Enablers to Accessing Rehabilitation That Meets Their Needs: The Rehabilitation Choices Study, Part 2—Consumer Perspectives
Authors: Mason Gillian , Ribbons Karen , Bailey Lucy , O'Malley Adrian , Ward Tracy , Ward Stephen , Pollack Michael , Walker Frederick R. , Nilsson Michael , Hodyl Nicolette 
Published: September 2024

ABSTRACTIntroductionImproved access to rehabilitation is highlighted as a key pathway to achieving the World Health Organisation's (WHO) goal of ensuring healthy lives and promoting well‐being for all (Sustainable Development Goal 3). This article is the second in a two‐part series outlining the findings from the Rehabilitation Choices study, which aimed to identify how health professionals and consumers in Australia are informed to make decisions about rehabilitation, and their experience with barriers and enablers to accessing that rehabilitation. In this study, we present the perspectives of consumers with different health conditions and a range of experiences with rehabilitation services.MethodsThis was a qualitative study using focus groups and semi‐structured interviews. People with self‐reported lived experience of rehabilitation and carers were recruited using maximum variation sampling. Thematic analysis of data was conducted using an inductive approach.ResultsFifty‐six consumers with diverse lived experiences of rehabilitation (19–80 years, 49 patients, 7 carers) participated in focus groups and interviews to discuss how they sourced information about rehabilitation and their experiences of what made it hard or easy to access rehabilitative care to meet their needs. Four themes were produced from the data: (1) service‐centricity of options limits access, (2) access is the patient's responsibility, (3) enabling decision‐making about rehabilitation with appropriate information and (4) provision of a psychologically safe environment.ConclusionsAny planned (re)design of services to improve consumer access to rehabilitation should consider the themes identified in this study. This will ensure that consumers are provided with rehabilitation options that suit their holistic and unique needs beyond consideration of their medical diagnoses, and are actively supported to navigate this access, provided with information to help them make informed choices and provided a psychologically safe environment to engage effectively with rehabilitation.Patient or Public ContributionThree consumer research partners with lived experience of rehabilitation as patients or carers were core team members. They were involved in the design and implementation of the recruitment and communications strategies, design of the interview approach and discussion guide, contributed to the interpretation and contextualisation of findings and writing of this manuscript and are included as co‐authors (A. O., T. W. and S. W.).
Inhibition of circular JUN prevents the proliferation and invasion of glioblastoma via miR‐3064‐IGFBP5 axis
Authors: Zhang Yuhao , Liu Shiming , Wu Cheng , Gao Xin , Zhao Hongtao , Li Ou , Gao Faliang 
Published: September 2024

AbstractGlioblastoma (GBM) remains one of the most aggressive and lethal brain tumours, characterized by rapid progression and limited treatment options. This study investigated the regulatory roles of circular RNA circJUN, and its functional interaction with microRNA miR‐3064 in GBM pathogenesis. We employed bioinformatic analyses and clinical sample validation to identify circJUN as a potential target in GBM. Subsequently, we engineered GBM cell lines with stable circJUN knockout or overexpression, and transfected them with miR‐3064 mimic/inhibitor or IGFBP5 small interfering RNA (siRNA)/expression vector to elucidate the molecular mechanisms governing GBM proliferation and invasion. To investigate the in vivo effects, xenograft tumour models were established in nude mice using engineered cells to assess the roles of circJUN in tumour growth regulation. Our analyses revealed significant overexpression of circJUN in GBM tissues compared to healthy controls, which strongly correlated with poor patient prognosis. In vitro and in vivo experiments demonstrated that circJUN overexpression could enhance GBM cell proliferation and invasion. Mechanistic investigations uncovered EIF4A3 as an interacting factor of circJUN which promotes circJUN expression, and circJUN modulates miR‐3064 activity to regulate the malignancy of GBM cells. Furthermore, we identified IGFBP5, a crucial regulator of cell growth, as a direct target of miR‐3064, thereby establishing an additional layer of control over GBM proliferation and invasion. Our study unveils a complex regulatory network involving circJUN, miR‐3064 and IGFBP5 in GBM pathogenesis, underscoring their potential as novel therapeutic targets for improving patient outcomes. Our findings not only contribute to the understanding of GBM biology but also pave the way for innovative therapeutic approaches in the management of this malignancy.
Thurstonian Models for the Duo‐Trio and Its Variants
Authors: Bi Jian , Kuesten Carla 
Published: September 2024

ABSTRACTThe duo‐trio is one of the basic sensory discrimination methods. There are various variants of the duo‐trio including the double duo‐trio (DDT), the dual reference duo‐trio (DRDT), the paired duo‐trio with AB and BA pairs (PDTAB), and the paired duo‐trio with same (AA or BB) and different (AB or BA) pairs (PDTSD). Thurstonian models, that is, psychometric functions for the duo‐trio and its four variants, DDT, DRDT, PDTAB, and PDTSD, are reviewed and developed in this paper. The theorized performances, that is, statistical powers of the four variants were explored and compared with the conventional duo‐trio. R codes are developed and provided for analyses of the data of the duo‐trio and its variants.
Utility of Cardiac Magnetic Resonance in Assessing Arrhythmic Risk in Patients With Nonischemic Cardiomyopathy Undergoing Biventricular Pacing
Authors: El‐Damaty Ahmed , Sayed Mohamed , El‐Maghawry Mohamed , Kandil Hossam , Hassan Mohamed 
Published: September 2024

ABSTRACTBackgroundNonischemic cardiomyopathy (NICM) is responsible for approximately one‐third of heart failure and is associated with significant morbidity and mortality. Recent data suggested the lack of mortality reduction from adding a defibrillator to cardiac resynchronization therapy (CRT) in all patients with NICM. Myocardial fibrosis detected by cardiac magnetic resonance late gadolinium enhancement (CMR‐LGE) can help risk stratify patients who would benefit from adding a defibrillator to CRT in this patient population.ObjectivesWe aim to assess the relationship between the presence of myocardial fibrosis detected by CMR‐LGE and the rate of major arrhythmic events (MAE) that included sustained ventricular tachycardia (VT), appropriate cardiac resynchronization therapy‐defibrillator (CRT‐D) intervention, ventricular fibrillation (VF), and sudden cardiac death (SCD) in patients with NICM undergoing CRT and to compare all‐cause mortality and heart failure improvement between patients receiving cardiac resynchronization therapy‐pacing (CRT‐P) versus those receiving CRT‐D based on the presence of myocardial fibrosis.MethodsAll consecutive patients with NICM satisfying a guideline‐directed indication for CRT implantation were included in the study after excluding patients who refused to consent, patients with acute decompensated heart failure, and those contraindicated for a cardiac magnetic resonance (CMR). Patients were divided into two groups based on the presence of fibrosis in cardiac MRI: the LGE/CRT‐D group and the No LGE/CRT‐P group. They
were then followed for 1 year.ResultsSixty patients were enrolled. Sixteen patients (26.6%) developed MAE during the study duration, among those patients, seven had myocardial fibrosis (receiving CRT‐D as per protocol), while nine had no myocardial fibrosis (receiving CRT‐P as per protocol), (41.2% vs. 20.9%, p = 0.045). The presence of CMR‐LGE, regardless of the extent and distribution, predicted MAE with an odds ratio of 2.6 (CI = 1.78–8.9, p = 0.04). The presence of ≥7.5% of myocardial fibrosis by CMR was associated with 54% sensitivity and 100% specificity for MAE in the study population. All‐cause mortality was significantly higher in the No LGE/CRT‐P group versus the LGE/CRT‐D group (15 [34.9%] vs. 2 [11.8%], p = 0.076).ConclusionIn patients with NICM candidates for biventricular pacing, the presence of LGE on CMR, irrespective of the extent or segmental pattern, is independently associated with an MAE and is associated with worse heart failure outcomes. However, the absence of LGE did not rule out MAE, and implanting CRT‐P based on lack of fibrosis may result in higher all‐cause mortality.
Maternal social deprivation and preterm birth: The PreCARE cohort study
Authors: Gottardi Elsa , Lorthe Elsa , Schmitz Thomas , Mandelbrot Laurent , Luton Dominique , Estellat Candice , Azria Elie 
Published: September 2024

AbstractBackgroundMaternal exposure to unfavourable social conditions is associated with a higher rate of perinatal complications, such as placental vascular pathologies. A higher risk of preterm birth (PTB) has also been reported, and variations across studies and settings suggest that different patterns may be involved in this association.ObjectiveTo assess the association between maternal social deprivation and PTB (overall and by phenotype).MethodsWe analysed 9365 patients included in the PreCARE cohort study. Four dimensions (social isolation, insecure housing, no income from work and absence of standard health insurance) defined maternal social deprivation (exposure). They were considered separately and combined into a social deprivation index (SDI). The associations between social deprivation and PTB <37 weeks (primary outcome) were analysed with univariable and multivariable log‐binomial models (adjusted for maternal age, parity, education level and birthplace). Then we used multinomial analysis to examine the association with preterm birth phenotypes (secondary outcome): spontaneous labour, preterm prelabour rupture of membranes (PPROM) and placental vascular pathologies.ResultsIn all, 66.3%, 17.8%, 8.9% and 7.0% of patients had an SDI of 0, 1, 2 and 3, respectively. Social isolation affected 4.5% of the patients, insecure housing 15.5%, no income from work 15.6% and no standard health insurance 22.4%. Preterm birth complicated 7.0% of pregnancies (39.8% spontaneous labour, 28.3% PPROM, 21.8% placental vascular pathologies and 10.1% other phenotypes). Neither the univariable nor multivariable analyses found any association between social deprivation and the risk of preterm birth overall (SDI 1 versus 0: aRR 1.02, 95% confidence interval [CI] 0.83, 1.26; 2 versus 0: aRR 1.05, 95% CI 0.80, 1.38; 3 versus 0: aRR 0.92, 95% CI 0.66, 1.29) or its different phenotypes.ConclusionsIn the French PreCARE cohort, we observed no association between markers of social deprivation and the risk of preterm birth, regardless of phenotype.
I paid into it with every paycheck I earned: How benefit type and beneficiary contributions shape attitudes about increasing or decreasing administrative burdens for social protections
Authors: Haeder Simon F. , Sylvester Steven 
Published: September 2024

AbstractAdministrative burdens are an important policy tool that has received growing scholarly attention. Burdens are consequential and serve as a contributor to incomplete program take‐up. However, our knowledge is limited on how program characteristics affect public attitudes toward burden‐increasing or decreasing policies. Based on our knowledge of public attitudes toward the welfare state, two such characteristics, whether benefits are “earned” and whether they come in the form of in‐kind services or cash payments, may also affect perceptions of administrative burdens. Using a nationally representative survey (N = 2904), we tested support for two administrative burdens (in‐person interviews and requirements for government‐issued documents) and two administrative easings (presumptive eligibility and express lane eligibility) for Social Security, Medicare, Medicaid, and Temporary Assistance for Needy Families (TANF) in the United States. In general, we found no strong and consistent differences along both dimensions. However, Republicans, Conservatives, and those high in racial resentment consistently favored increasing burdens and opposed decreasing burdens with the opposite effect for Democrats, Liberals, and those low in racial resentment. Americans supported administrative burdens in the form of documentation requirements across all programs. However, they were open to burden decreases.
Bivariate GWA mapping reveals associations between aliphatic glucosinolates and plant responses to thrips and heat stress
Authors: Arouisse Bader , Thoen Manus P.M. , Kruijer Willem , Kunst Jonathan F. , Jongsma Maarten A. , Keurentjes Joost J.B. , Kooke Rik , de Vos Ric C.H. , Mumm Roland , van Eeuwijk Fred A. , Dicke Marcel , Kloth Karen J. 
Published: September 2024

SUMMARYAlthough plants harbor a huge phytochemical diversity, only a fraction of plant metabolites is functionally characterized. In this work, we aimed to identify the genetic basis of metabolite functions during harsh environmental conditions in Arabidopsis thaliana. With machine learning algorithms we predicted stress‐specific metabolomes for 23 (a)biotic stress phenotypes of 300 natural Arabidopsis accessions. The prediction models identified several aliphatic glucosinolates (GLSs) and their breakdown products to be implicated in responses to heat stress in siliques and herbivory by Western flower thrips, Frankliniella occidentalis. Bivariate GWA mapping of the metabolome predictions and their respective (a)biotic stress phenotype revealed genetic associations with MAM, AOP, and GS‐OH, all three involved in aliphatic GSL biosynthesis. We, therefore, investigated thrips herbivory on AOP, MAM, and GS‐OH loss‐of‐function and/or overexpression lines. Arabidopsis accessions with a combination of MAM2 and AOP3, leading to 3‐hydroxypropyl dominance, suffered less from thrips feeding damage. The requirement of MAM2 for this effect could, however, not be confirmed with an introgression line of ecotypes Cvi and Ler, most likely due to other, unknown susceptibility factors in the Ler background. However, AOP2 and GS‐OH, adding alkenyl or hydroxy‐butenyl groups, respectively, did not have major effects on thrips feeding. Overall, this study illustrates the complex implications of aliphatic GSL diversity in plant responses to heat stress and a cell‐content‐feeding herbivore.
Integrating multiregulatory analysis reveals the negative regulatory function of miR482a in the response of poplar to canker pathogen infection
Authors: Yang Xiaoqian , Yu Ruen , Liu Jiahao , Xiao Dandan , Wang Chun , Fu Tiantian , Yang Yuzhang , Rong Kaijing , Wang Yanwei 
Published: September 2024

SUMMARYCanker disease caused by the bacterium Lonsdalea populi is one of the most destructive diseases affecting poplar stems. However, the detailed stress response mechanisms of poplar have not been widely characterized. To explore the diverse regulatory RNA landscape and the function of key regulators in poplar subjected to L. populi stress, we integrated time‐course experiment with mock‐inoculation (CK) and inoculation (IN) with L. populi at the first, third, and sixth day (IN1, IN3, IN6) on Populus × euramericana cv. ‘74/76’ (107), small RNA‐seq, whole transcriptome‐wide analysis, degradome analysis and transgenic experiments. A total of 98 differentially expressed (DE) miRNA, 17 974 DEmRNA, and 807 DElncRNA were identified in poplar infected by L. populi, presenting dynamic changes over the infection course. Regulatory networks among RNAs were further constructed. Notably, a network centered on ptc‐miR482a in CK‐vs‐IN3 contained most DEGs. We show that miR482a and miR1448 are located in one transcript as a polycistron. Overexpression of pre‐miR482a‐miR1448 (OX482‐1448) and pre‐miR482a (OX482) increased poplar susceptibility to canker pathogen with reduced accumulation of reactive oxygen species, while the suppression of miR482a (STTM482) conferred poplar disease resistance. PHA7 was validated as the target of miR482a with degradome sequencing and tobacco transient co‐transformation, its expression being downregulated in OX482‐1448 and OX482 lines. Additionally, a series of phasiRNAs were triggered by miR482a targeting PHA7, forming regulatory cascades with more RLP, NBS‐LRR, and PK genes, further verifying the defense function of miR482a. These findings provide insights for understanding the roles of ncRNAs and regulatory networks involved in poplar immunity.
Dual ablation of the RyR2‐Ser2808 and RyR2‐Ser2814 sites increases propensity for pro‐arrhythmic spontaneous Ca2+ releases
Authors: Janicek Radoslav , Camors Emmanuel M. , Potenza Duilio M. , Fernandez‐Tenorio Miguel , Zhao Yanting , Dooge Holly C. , Loaiza Randall , Alvarado Francisco J. , Egger Marcel , Valdivia Hector H. , Niggli Ernst 
Published: September 2024

AbstractDuring exercise or stress, the sympathetic system stimulates cardiac contractility via β‐adrenergic receptor (β‐AR) activation, resulting in phosphorylation of the cardiac ryanodine receptor (RyR2). Three RyR2 phosphorylation sites have taken prominence in excitation–contraction coupling: S2808 and S2030 are described as protein kinase A specific and S2814 as a Ca2+/calmodulin kinase type‐2‐specific site. To examine the contribution of these phosphosites to Ca2+ signalling, we generated double knock‐in (DKI) mice in which Ser2808 and Ser2814 phosphorylation sites have both been replaced by alanine (RyR2‐S2808A/S2814A). These mice did not exhibit an overt phenotype. Heart morphology and haemodynamic parameters were not altered. However, they had a higher susceptibility to arrhythmias. We performed confocal Ca2+ imaging and electrophysiology experiments. Isoprenaline was used to stimulate β‐ARs. Measurements of Ca2+ waves and latencies in myocytes revealed an increased propensity for spontaneous Ca2+ releases in DKI myocytes, both in control conditions and during β‐AR stimulation. In DKI cells, waves were initiated from a lower threshold concentration of Ca2+ inside the sarcoplasmic reticulum, suggesting higher Ca2+ sensitivity of the RyRs. The refractoriness of Ca2+ spark triggering depends on the Ca2+ sensitivity of the RyR2. We found that RyR2‐S2808A/S2814A channels were more Ca2+ sensitive in control conditions. Isoprenaline further shortened RyR refractoriness in DKI cardiomyocytes. Together, our results suggest that ablation of both the RyR2‐Ser2808 and RyR2‐S2814 sites increases the propensity for pro‐arrhythmic spontaneous Ca2+ releases, as previously suggested for hyperphosphorylated RyRs. Given that the DKI cells present a full response to isoprenaline, the data suggest that phosphorylation of Ser2030 might be sufficient for β‐AR‐mediated sensitization of RyRs.
imageKey points
Phosphorylation of cardiac sarcoplasmic reticulum Ca2+‐release channels (ryanodine receptors, RyRs) is involved in the regulation of cardiac function.
Ablation of both the RyR2‐Ser2808 and RyR2‐Ser2814 sites increases the propensity for pro‐arrhythmic spontaneous Ca2+ releases, as previously suggested for hyperphosphorylated RyRs.
The intra‐sarcoplasmic reticulum Ca2+ threshold for spontaneous Ca2+ wave generation is lower in RyR2‐double‐knock‐in cells.
The RyR2 from double‐knock‐in cells exhibits increased Ca2+ sensitivity.
Phosphorylation of Ser2808 and Ser2814 might be important for basal activity of the channel.
Phosphorylation of Ser2030 might be sufficient for a β‐adrenergic response.