bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Throughput Optimization of Paper-Based Cell-Free Biosensors
Authors: Garcia David C. , Davies John P. , Davidson Charles E. , Phillips Daniel A. , Miklos Aleksandr E. , Lux Matthew W. 
Published: October 2024

AbstractCell-free expression systems maintain core cellular processes without intact cells and offer attractive properties as point-of-need biosensors. The ability to lyophilize, store, and use on-demand make these sensors usable in the field, and the lack of membranes means that there are no analyte transport issues and that new sensors can be deployed by simply adding a different DNA molecule. The lack of membranes also means that sensor designs and reaction optimizations can be screened in high throughput. While shelf stability has been demonstrated in specific cases using additives, these approaches are not universal to the myriad cell-free expression methods and formats. Here, we present new high-throughput screening methods to optimize cell-free expression formulations when embedded into paper for use as sensors. Our method leverages acoustic liquid handling to dispense reactions onto 384-well paper ticket formats and machine vision to quantify reaction performance from a colorimetric reporter enzyme. The throughput enabled shifts the bottleneck from experimental execution to selecting the experiments to execute; we therefore implement design-of-experiments to optimize the information gained from each design-build-test-learn cycle. We used these approaches to first optimize the performance of a low-cost cell-free expression formulation that was initially non-functional when embedded in paper, then further optimize it for tolerance to exposure to heat. With only 2 rounds of experimentation lasting 4 days total for each goal, the result are an energy mixture with 8% of the materials cost of a commonly used version and a formulation of excipients that maintain 60% of activity after 6 hours of storage at 50 °C and. Finally, we showcase the use of the cost-optimized formulation in a 3D-printed paperfluidic device where it outperforms the standard formulation at much lower cost.
Psychosocial factors contributing to a syndemic of substance use and HIV risk among Floridian transgender women
Authors: Sullivan Daniell , Clarke Rachel , Wilson Hannah , Dévieux Jessy , Cyrus Elena 
Published: October 2024

AbstractBackgroundTransgender women are at heightened risk of HIV and substance misuse due to systemic discrimination and structural healthcare barriers at the macro, meso, and micro levels. Floridian Transgender Women (FTW) face unique challenges in an environment with limited resources and inconsistent epidemiological data. Syndemic theory, which examines how co-occurring conditions like HIV and substance use disorders (SUD) are driven by large-scale factors, has not been fully explored in FTW.ObjectiveThis study aimed to identify psychosocial factors contributing to substance use and HIV risk from a syndemic perspective among FTW.MethodsFrom May 2021 to November 2023, over 500 at-risk FTW were recruited from community partner agencies in Miami-Dade and Orlando counties for a larger prospective study. Univariate analysis identified psychosocial factors, while bivariate analysis explored their contributions to the syndemic of substance misuse and HIV risk.ResultsOut of 160 participants screened, 55.6% were ineligible, leaving 89 enrolled. Participants ranged in age from 18 to 63, with 25% identifying as people of color. Mental illness was reported by 62.9%, with depression and anxiety being the most common diagnoses. However, only 29.2% had accessed mental health care in the past year. Despite high rates of substance use—48.8% reported drug use during sex, 46.1% likely had an SUD, and 25.8% reported hazardous drinking—68.9% perceived their quality of life as high. Drug use during sex was significantly associated with intimate partner violence (IPV) (p=.01) and perceived quality of life (p=.003).ConclusionFTW face significant challenges, including discrimination, mental illness, trauma, and IPV, which increase the risk of substance use, particularly marijuana and alcohol. Despite these challenges, many maintain a high perceived quality of life. The syndemic interaction calls for comprehensive interventions addressing financial, mental health, and accessibility barriers. Further research into socio-political stressors that exacerbate substance use and high-risk behaviors is recommended.
A composite socioeconomic deprivation index fromAll of Ussurvey data: associations with health outcomes and disparities
Authors: Gupta Sonali , Lam Vincent , Jordan I. King , Mariño-Ramírez Leonardo 
Published: October 2024

ABSTRACTSocioeconomic deprivation – defined as a lack of social, economic and material resources – is associated with poor health outcomes and health disparities between population groups. TheAll of UsResearch Program is a longitudinal cohort study of diverse participants from the United States, with demographic and social determinants of health data gleaned from participant surveys and health outcome data derived from electronic health records. We developed a composite index of socioeconomic deprivation (iSDI) using a cohort of 202,919All of Usparticipants – based on education, employment, health insurance, housing, and income data – and we associated iSDI with health outcomes and disparities. iSDI is significantly associated with 970 out of 1,755 (55.3%) health conditions modeled here, with 661 positive and 309 negative associations. Mental disorders and circulatory diseases show the highest proportion of positive associations with iSDI, whereas neoplasms and congenital anomalies show the highest proportion of negative associations. Black (0.55) and Hispanic (0.52)All of Usparticipants show higher average iSDI values compared to White (0.29) and Asian (0.24) participants; although the majority of iSDI variation is found within (76.8%) rather than between (23.2%) groups. iSDI mediates 213 out of 399 (53.5%) Black health disparity conditions and 173 out of 297 (58.2%) Hispanic health disparity conditions. The composite socioeconomic deprivation index (iSDI) developed here is associated with a wide variety of health outcomes and disparities in theAll of Uscohort, and we make participant iSDI values available on the Researcher Workbench to support future studies on social determinants of health.
Narratives for positive nature futures in Europe
Authors: D’Alessio Alessandra , Fornarini Claudia , Fernandez Nestor , Namasivayam Anandi Sarita , Visconti Piero , Dertien Jeremy , Hällfors Maria , Jung Martin , Moreira Francisco , O’Connor Louise , Osti Matea , Quintero-Uribe Laura C. , Viti Martina Marei , Pereira Henrique M. , Verburg Peter H. , Rondinini Carlo 
Published: October 2024

AbstractThe Nature Futures Framework (NFF) is a novel tool for the development of positive scenarios centred on the relationship of nature and people, emphasising biodiversity as part of the solution to environmental challenges across various spatial and temporal scales, explicitly addressing a plurality of values for nature.In this work, we describe the process that has led to the formulation of continental-scale positive narratives for conservation in Europe based on the NFF and its value perspectives (Nature for Nature; Nature for Society; Nature as Culture), through a stakeholder group elicitation. We focused on 6 topics in the narratives: Nature Protection and Restoration; Forestry; Freshwater Ecosystems; Urban Systems; Agriculture, and Energy. We analyse differences and similarities among the narratives across these topics.We develop three novel Nature Futures narratives for Europe with contrasting perspectives and priorities for the six topics. Within the EU socioeconomic trends and policy framework, common solutions that simultaneously tackle biodiversity conservation and instrumental and cultural Nature’s Contributions to People (NCP) provision emerged.This set of narratives may integrate preferences concerning EU-level conservation targets and plausible socio-ecological development pathways, supporting the modelling of positive scenarios for nature that can be crucial in guiding policy decisions towards recovery of nature.
Temperate alley‐cropping agroforestry improves pest control potential by promoting spider abundance and functional diversity
Authors: Matevski Dragan , Sagolla Viktoria , Beule Lukas , Schuldt Andreas 
Published: October 2024

Abstract

Intensive agricultural land use negatively impacts biodiversity, including arthropod predator diversity and their pest control potential. Alley‐cropping agroforestry systems (integration of tree rows into arable land) are increasingly considered an economically viable alternative for more sustainable and biodiversity‐friendly agriculture. However, their effectiveness in promoting generalist predator diversity and pest control, especially as a function of space (distance from tree rows) and time (across the growing season) remains poorly understood.

We assessed how spider abundance, taxonomic and functional diversity (as proxies of pest control potential) respond to temperate alley‐cropping agroforestry systems as compared to open croplands. Additionally, we analysed whether spiders with different habitat preferences (eurytopic, forest and open‐habitat specialists) show distinct responses. Lastly, we analysed whether the influence of agroforestry changes with decreasing tree proximity and across the growing season.

Tree row proximity generally benefited the abundance, as well as taxonomic and functional diversity of the overall spider communities. Alley‐cropping promoted the abundance and taxonomic diversity of forest specialists and eurytopic spiders, without negatively affecting open‐habitat specialists. The positive effect of alley cropping was strongest within tree rows and their immediate vicinity, but was still detectable at considerably farther distances. These patterns were temporally dynamic with spider abundance and taxonomic diversity being highest within tree rows in early spring and spiders spilling over to adjacent crop rows in subsequent periods.

Synthesis and applications: Overall, our findings highlight that the inclusion of tree rows benefited spiders across the entirety of the 48 m crop rows, independent of crop type. Since the peak in spider abundance and taxonomic diversity in crop rows coincided with crop pest arrival, alley‐cropping agroforestry is expected to benefit agricultural production through increased pest control potential. Alley‐cropping agroforestry may therefore be an important management strategy to develop more sustainable agricultural systems benefiting farmers and biodiversity alike.
Chitosan coatings containing thyme essential oil enhance the quality of snakehead (Channa striata) during chilled storage verified by metabolomics approaches
Authors: Liu Yi , Geng Chenhan , Zeng Huiduan , Kai Yi , Lu Yuyun 
Published: October 2024

AbstractIn the present study, we investigated the preservative effects of chitosan (CS) coatings, with and without thyme essential oil (TEO), combined with vacuum impregnation (VI) on maintaining the quality of snakehead fillets during chilled storage. The results showed that the VI treatment significantly inhibited drip loss, discoloration, microbial proliferation, and the accumulation of biogenic amines (BAs) in the sneakhead fillets. Compared to the control, the fillets treated with VI of 1% (w/w) CS and 1.5% (w/w) TEO (i.e., CSTEO) showed significant reductions in both psychrophiles and mesophiles, with a 2.66 log CFU/g decrease in total viable count (TVC) on day 3 and a 1.89 log CFU/g decline in TVC on day 9, respectively. In addition, the content of histamine and putrescine in the CSTEO groups was maintained at ∼1.14 and 3.23 mg/kg during the 12‐day chilled storage, respectively. A total of 100 chemical compounds were tentatively identified using untargeted metabolomics approaches. The multivariate analysis further revealed that the combination of VI and CSTEO maintained fish quality mainly through preventing lipid oxidation and protein degradation. Overall, the VI‐CSTEO treatment effectively maintained the fish quality during storage at 4°C, with minimum microbial proliferation and accumulation of BAs.Practical ApplicationThe preservative effect of chitosan coatings containing thyme essential oil combined with vacuum impregnation on snakehead quality during the 12‐day chilled storage was verified, and the underlying mechanisms were deciphered through integrated metabolomics approaches. Our study could provide a promising strategy for the preservation of aquatic products.
Abolishing structural domination: US Reconstruction and the double bind of emancipation
Authors: Gorup Michael 
Published: October 2024

AbstractWhat does it take to abolish structural domination? If domination is structural, then it can only be eliminated by the transformation or abolition of structures. For this to happen, agents advantaged by existing structures will often have to be brought under a form of power that lays beyond their control. Defeating domination therefore seems to require the embrace of dominating power. I call this dilemma the double bind of emancipation. To illustrate, I turn to debates regarding land confiscation during the Reconstruction era in the United States (1865–1877). Confiscation was intended to destroy the material bases of planter power and guarantee formerly enslaved people economic independence. But it would also entail subjecting planters to uncontrolled power. To address the double bind, I argue emancipation must be guided by a social theory of domination. Emancipation from structural domination requires identifying and eliminating the conditions that reproduce domination over time.
Exploring the impact of sexual positions on ejaculation: Insights from a survey study by the Andrology Working Group of the Society of Urological Surgery in Turkey
Authors: Gül Murat , Şahin Ali , Doğan Çağrı , Çeker Gökhan , Altıntaş Emre , Deliktaş Hasan , Demir Murat , Yavuz Aldülmecit , Altunkol Adem , Değer Doğan , Kaynar Mehmet , Duran Berkan , Toprak Tuncay , Bahçeci Tuncer , Gül Ümit 
Published: October 2024

AbstractBackgroundSexual position is one of the several aspects that affects ejaculation control, which is essential for sexual pleasure. Still little is known, nevertheless, about the connection between sexual positions and ejaculation duration.ObjectiveTo investigate the impact of various sexual positions on the duration of ejaculation and gain a deeper understanding of the elements that influence the ability to control ejaculation.MethodAn online survey was carried out on a sample of 1904 heterosexual men between the ages of 18–65 years. Premature ejaculation (PE) diagnostic tool was used to define PE. Demographic, behavioral, and physiological traits that are linked to PE and non‐PE groups were collected. The participants listed their preferred sexual positions and the ones they changed to during ejaculating. Also, analyses were performed between male/female active or deep/shallow thrust positions and PE status.ResultsAlthough there were no appreciable variations in age or circumcision between PE and non‐PE groups, the age of first sexual experience was associated with PE status. Groups also varied in the number of weekly ejaculations, the duration of the favored ejaculations, and the characteristics of the erection. The most preferred sexual posture was the doggy style regardless of the group. When the non‐PE group felt to ejaculate, they preferred to change the position significantly more than the PE group (74% vs 67.2%; p < 0.05). However, when participants felt ejaculate, non‐PE participants tended to switch to shallow thrusting positions significantly more than PE participants, who preferred deeper positions (27.1% vs. 18%; p < 0.05).ConclusionThis study underlines the relevance of considering sexual positions in controlling PE. Modifying positions during sexual intercourse may offer a non‐pharmacological therapeutic alternative for improving ejaculation control. Future studies in this field might help to create tailored PE treatment strategies.
Gender, SSE and French public policies for ageing at home
Authors: Dussuet Annie , Gasté Louise 
Published: October 2024

AbstractIn France as in other countries, there is a consensual preference by public authorities, old people and their families to stay at home as long as possible. This means the necessity of care work carried out informally by relatives, or formally by professionals. Women are the main providers of care, formal or informal, and social and solidarity economy (SSE) organizations are widely involved in public policies for ageing at home. This raises questions about the role played by the associations, in the promotion of gender equality. The paper presents French context and our analytical framework about formalization of care: first, the ongoing professionalization of employees in oldest home care associations yet facing recurring funding difficulties; second, the emergence of new ‘peripheral’ associative services to ‘help family caregivers’, that tinker specific regulations to consider the specificities of care work. Then, using some results of two field surveys, we show how home‐based care associations had to define the boundaries between more and less ‘essential’ work, paid and unpaid care, and how this division between material and relational tasks, associated with ‘new innovative’ working frameworks, hybrid between voluntary work and salaried employment, risk to deny the reality of work in caring, and through that, gender equality.
Economic burden of hospital admissions for adverse drug reactions in France: The IATROSTAT‐ECO study
Authors: Laroche Marie‐Laure , Tarbouriech Noémie , Jai Taha , Valnet‐Rabier Marie‐Blanche , Nerich Virginie 
Published: October 2024

AimsHospitalizations for adverse drug reactions (ADR‐HA) have increased over the last decade, but the impact of ADR‐HA has rarely been evaluated. The aim of this study was to estimate the economic burden of ADR‐HA in France.MethodsA partial economic evaluation from the viewpoint of French public health insurance was performed, based on a previous pharmacovigilance study (IATROSTAT) performed in 2018 in public hospital medical units. The cost included direct medical costs, collected retrospectively, from the French hospital discharge database. The economic burden was estimated by calculating the total cost per ADR‐HA patient (cost of hospital stays, additional daily cost of specific stays, such as in a resuscitation, intensive care or continuous surveillance unit, drug products and medical devices in addition to Healthcare Resource Group‐based tariffs, and specific outpatient consultations and other clinical and technical medical procedures, over 3 months as from the first day of ADR‐HA). The robustness of the results was assessed using a one‐way deterministic sensitivity analysis of cost factors applying tariffs from 2023 instead of 2018.ResultsAccording to the 2018 tariffs (vs. 2023), the mean total cost per patient with ADR‐HA was estimated at €5208 ± €3719 (vs. €5974 ± €4232) ranging from €514 to €23 355 (vs. €618 to €27 380). The total cost for 196 patients with ADR‐HA admitted to a sample of French public hospitals was estimated at €1 020 549 (vs. €1 170 960). It could be estimated at €1.3 billion at the national level.ConclusionsIn addition to the increase in the number of expensive drugs, the ageing population and polypharmacy, the economic impact of serious ADR‐HA weighs heavily on healthcare spending.
Predicting remission after acute phase pharmacotherapy in patients with bipolar I depression: A machine learning approach with cross‐trial and cross‐drug replication
Authors: Marrero‐Polanco Jean , Joyce Jeremiah B. , Grant Caroline W. , Croarkin Paul E. , Athreya Arjun P. , Bobo William V. 
Published: October 2024

AbstractObjectivesInterpatient variability in bipolar I depression (BP‐D) symptoms challenges the ability to predict pharmacotherapeutic outcomes. A machine learning workflow was developed to predict remission after 8 weeks of pharmacotherapy (total score of ≤8 on the Montgomery Åsberg Depression Rating Scale [MADRS]).MethodsSupervised machine learning models were trained on data from BP‐D patients treated with olanzapine (N = 168) and were externally validated on patients treated with olanzapine/fluoxetine combination (OFC; N = 131) and lamotrigine (LTG; N = 126). Top predictors were used to develop a prognosis rule informing how many symptoms should change and by how much within 4 weeks to increase the odds of achieving remission.ResultsAn AUC of 0.76 (NIR:0.59; p = 0.17) was established to predict remission in olanzapine‐treated subjects. These trained models achieved AUCs of 0.70 with OFC (NIR:0.52; p < 0.03) and 0.73 with LTG (NIR:0.52; p < 0.003), demonstrating external replication of prediction performance. Week‐4 changes in four MADRS symptoms (reported sadness, reduced sleep, reduced appetite, and concentration difficulties) were top predictors of remission. Across all pharmacotherapies, three or more of these symptoms needed to improve by ≥2 points at Week‐4 to have a 65% chance of achieving remission at 8 weeks (OR: 3.74, 95% CI: 2.45–5.76; p < 9.3E‐11).ConclusionMachine learning strategies achieved cross‐trial and cross‐drug replication in predicting remission after 8 weeks of pharmacotherapy for BP‐D. Interpretable prognoses rules required only a limited number of depressive symptoms, providing a promising foundation for developing simple quantitative decision aids that may, in the future, serve as companions to clinical judgment at the point of care.
The effects of special educational needs and socioeconomic status on teachers' and parents' judgements of pupils' cognitive abilities
Authors: Smeets Kim , Rohaan Ellen , van der Ven Sanne , Bakx Anouke 
Published: October 2024

AbstractBackgroundTeachers' and parents' judgements of pupils' cognitive abilities influence pupils' daily learning opportunities and experiences, as these judgements affect the difficulty level of materials and instruction that teachers and parents provide. Over time, these judgements thus significantly shape educational success. However, pupils' characteristics, such as special educational needs (SEN), giftedness and socioeconomic status (SES) can influence and bias judgement accuracy.AimsThe present study aimed to investigate the relation between pupils' cognitive abilities and their teachers' and parents' judgements of these abilities, and potential bias in these judgements related to SEN, giftedness, and SES.SampleThe sample consisted of 1073 primary school pupils from grades 4–6 from 77 classes in 16 schools, and their teachers and parents.MethodsTeachers and parents rated their pupils' cognitive abilities. Pupils completed the COVAT‐3, a cognitive ability test.ResultsMultilevel analyses revealed that parent judgements were significantly higher than teacher judgements, but both informants' judgements were equally strong related to the cognitive ability scores. When controlling for pupils' assessed cognitive abilities, the results revealed small judgement biases: negative for SEN, positive for giftedness, and finally positive for high SES, but only in teachers.ConclusionsOverall, the results indicated that teachers and parents can judge their pupils abilities to a moderate degree, but they also hold judgement biases related to SEN, giftedness and SES. As these biases can affect pupils' opportunities, it is important to increase teachers' and parents' awareness.