bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering a broad-spectrum multi-epitope vaccine to combat emerging monkeypox virus by immunoinformatic approaches
Authors: Puagsopa Japigorn , Jumpalee Panuwid , Lohasupthawee Pana , Sutjaritvorakul Thanawat , Meksiriporn Bunyarit 
Published: September 2024

Abstract

Monkeypox virus (MPXV), has caused 41,664 confirmed cases and five deaths in non-endemic regions, as reported by the World Health Organization (WHO). There is an urgent demand for effective vaccines to combat and prevent the spread of MPXV. Traditional vaccine development is low-throughput, expensive, time-consuming, and susceptible to reversion to virulence. As an alternative, a reverse vaccinology approach can be employed as a promising tool to design effective and safe vaccines against MPXV. Here, MPXV proteins associated with viral infection were analyzed for potential immunogenic epitopes to design multi-epitope vaccine constructs based on B-cell, CD4+, and CD8+ epitopes. Epitopes were selected based on allergenicity, antigenicity, and toxicity parameters. The prioritized epitopes were then combined via peptide linkers and N-terminally fused to various protein adjuvants, including PADRE, beta-defensin 3, 50S ribosomal protein L7/12, RS-09, and the cholera toxin B subunit (CTB). All vaccine constructs were further computationally validated for physicochemical properties, antigenicity potential, allergenicity, safety, solubility, and structural stability. The three-dimensional structure of the selected construct was also predicted. Moreover, molecular docking and molecular dynamics (MD) simulations between the vaccine and the TLR-4 immune receptor demonstrated a strong and stable interaction. The vaccine construct was codon-optimized for high expression in the E. coli platform and was finally cloned in silico into the pET21a(+) vector. Collectively, these results could represent innovative tools for vaccine formulation against MPXV and be transformative for other infectious diseases.
Vertebral Artery Web: A Case Report
Authors: Amer Lamar A. , Aljohani Abdullah K. , Alshanqiti Ahmed I. , Sheikh Bassem Y. 
Published: September 2024

Abstract

Fibromuscular dysplasia (FMD) presents a complex vascular challenge characterized by non-atheromatous changes in medium-sized arteries, primarily impacting the cervicocephalic territories. Vertebral artery webs, a rare manifestation of FMD, pose a significant risk, potentially leading to thromboembolisms and neurological deficits. We report a case of a 40-year-old female with multiple cerebral aneurysms successfully managed via endovascular intervention, subsequently revealing a progressive vertebral artery web. This case underscores the critical need for early detection, precise monitoring, and individualized management strategies. The discussion emphasizes the importance of multidisciplinary collaboration, advanced imaging techniques, and tailored therapeutic approaches. Recommendations include vigilant surveillance, personalized treatment strategies, optimized medical therapy, collaborative care, and further research to enhance awareness and understanding of this rare but impactful condition.
Assessment and Enhancement of Methods for Exosome Isolation from Camel Milk
Authors: 杨 回 , 乌伦 塔娜 
Published: September 2024

Abstract

Background
Camel milk is a source of exosomes with potential immunomodulatory and antioxidant benefits. However, the efficiency of exosome extraction from camel milk is crucial for maximizing their potential applications.
Objective
This study aims to optimize the exosome isolation process from camel milk to increase the yield and quality of the extracted exosomes.
Methods
We employed various pretreatment strategies prior to ultracentrifugation, including chymosin-assisted, isoelectric point (PI)-precipitation, and ethylenediaminetetraacetic acid (EDTA)-assisted methods. For the characterization of the isolated exosomes, we utilized nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blotting to evaluate size distribution, structural integrity, and specific exosomal protein markers, respectively.
Results
The chymosin-assisted technique yielded exosomes with a more intact and defined double-layered membrane structure, lower non-exosomal protein background, and a higher presence of the exosomal marker CD63 as evidenced by western blotting analysis (P < 0.05), compared with the other methodologies tested.
Conclusion
Chymosin pretreatment combined with ultracentrifugation significantly enhances the isolation of high-quality exosomes from camel milk, indicating that this approach may be the most effective for purifying exosomes for downstream biomedical applications. Further studies are encouraged to validate and refine this method for large-scale extraction.
The Impact of Effective Menopausal Symptom Management on Workplace Performance and Well-being: A Comprehensive Analysis
Authors: Ndindeng Atina 
Published: September 2024

Abstract

Menopausal symptom management is no longer just a personal health issue—it is a critical organizational concern that directly affects workplace performance, employee retention, and overall business success. This article provides a comprehensive analysis of the profound impact that effective management of menopausal symptoms can have on organizational outcomes. Utilizing a robust mixed-methods approach, this study integrates quantitative data from extensive surveys and qualitative insights from detailed interviews to evaluate the prevalence and effects of menopausal symptoms among working women. The findings reveal that inadequate management of menopausal symptoms can lead to substantial declines in productivity, increased absenteeism, and higher turnover rates, posing serious risks to organizational competitiveness and sustainability. However, the study also highlights the effectiveness of various management strategies, including innovative digital tools, and emphasizes the necessity of considering intersectional factors such as race and socioeconomic status. Crucially, this research underscores the urgent need for supportive workplace policies, not just as a matter of employee welfare but as a critical factor in maintaining organizational performance. The study concludes with actionable recommendations for organizations, emphasizing that the successful implementation of menopause support strategies must be tailored to cultural and global contexts to maximize their impact. Grounded in the latest 2024 research, this analysis positions menopause management as a vital element of future-focused human resource strategy.
Time-integrated and time-resolved wavelet coherence analysis to quantify cerebral autoregulation: Algorithm review, new development, and prediction of pediatric cardiac arrest
Authors: Yu Priscilla , Li Lin , Ranjitkar Sheetala , Zhou Yufeng , Raman Lakshmi , Liu Hanli 
Published: September 2024

Abstract

Wavelet transform and wavelet transform coherence (WTC) are advanced data analysis tools in the time-frequency domain for studying nonstationary time series and the relationship between two synchronous time series. These advanced tools can be used to quantify cerebral autoregulation (CAR) by calculating the mean arterial pressure (MAP) and regional cerebral oxygenation (StO2). The goal of this study was to develop a novel WTC analysis method that enables the quantification of disrupted CAR in pediatric cardiac arrest. Accordingly, this paper first provides a narrative explanation of a well-developed WTC computational package using a graphical flowchart for clinicians and life scientists to better comprehend the analysis rationale. Next, we introduce a new analysis strategy to quantify time-resolved significant coherence (trSC) which facilitates the visualization of temporal changes in CAR, followed by a clinical example using trSC to detect impaired CAR in pediatric cardiac arrest. Specifically, we quantified 25-hour temporal traces of trSC using concurrently recorded MAP and StO2 and examined CAR impairment during pre-, peri-, and post-cardiac arrest in 20 arrest events in 16 pediatric patients. This study demonstrated that trSC is a promising parameter for investigating CAR and can be developed as a bedside monitoring tool for clinicians.
Substance use research with gender minority populations: A systematic review of PubMed.
Authors: Barger Branden T. 
Published: September 2024

Abstract

Gender minority populations including transgender, genderqueer, gender non-binary, and other gender non-conforming groups, have increasingly become of special interest in healthcare and health-based research settings as populations that experience unique health disparities, strengths, and resiliencies. Though data supports high rates of substance use among both sexual and gender minority groups, there has been little work to summarize or report this data on a more global level. This review sought to examine literature and summarize non-alcohol or nicotine-related substance use research practices with a focus on characterizing methodologies for assessing gender identity, expression, and/or presentation and various substance use outcomes. Further, this review sought to highlight critical knowledge gaps in evaluating substance use among gender minority populations and propose improvements to current works.
Mitigating PFAS Contamination: Assessing the Impact of California's Legislation from 2018-2022 on Drinking Water Quality
Authors: Bagga Sameer Dutt , Parshley Iris J.N. , Tallon Lindsay 
Published: September 2024

Abstract

In California's public water systems, per- and polyfluoroalkyl substances (PFAS) contamination affects 65% of tested sites, impacting over 16 million residents [7]. This paper examines California's response to PFAS contamination through policy analysis and a comparative study of PFAS levels. The research reveals a significant reduction in PFAS contamination levels, suggesting the effectiveness of these policies. While recognizing limitations, this study underscores the urgency of addressing PFAS contamination and highlights California's approach as a model for other states. Future research should explore the mechanisms through which legislation can influence contamination levels, expand testing, and assess socio-economic implications, especially in environmental justice communities [3]. California's legislative efforts emphasize the collective resolve required to mitigate PFAS contamination's impact. This research contributes to the growing understanding of how policy interventions can address the pressing issue of PFAS contamination and calls for continued efforts to protect public health and the environment.
Macroeconomic Impacts of Energy Price Shocks and Monetary Policy: An Estimated DSGE Model for Turkey
Authors: Akpolat Ahmet Gökçe 
Published: September 2024

Abstract

This study examines the impact of energy price shocks and the role of monetary policy in the face of energy price shocks within a Bayesian DSGE model over the quarterly period 2009:1-2021:4 for Turkey. The results demonstrate that the main macroeconomic variables such as GDP, domestic production, employment, investments, real wages experience a decline and both core and CPI inflation an increase in the presence of international energy price energy price shocks. Moreover, the different scenarios of monetary policy responses to both core inflation and output deviations from their steady states which are strong, baseline and weak policy responses are illustrated and discussed. In the scenario in which monetary policy responds most strongly to deviation in the core inflation in the face of energy shocks; the decline in GDP, domestic production, investments and employment and the increases in core and CPI inflation are the highest while the increase in real wages are the lowest. In the scenario where monetary policy reacts strongly to the output deviation, the decrease in real wages and the increase in core and CPI inflation are experienced as the highest, while the decrease in GDP, domestic production, investments and employment turn out to be the lowest. These effects appear to differ in scenarios where monetary policy responds weaker to inflation and the output gap. As for a policy implication, the internationally coordinated contractionary monetary policy can be pursued to restrain the negative impacts of the international shocks.
Prevalence of and factors associated with cerebral malaria among children aged 6 to 59 months with severe malaria in Western Uganda: A hospital-based cross-sectional study
Authors: Mseza Banga , Kumbakulu Patrick Kumbowi , Nduwimana Martin , Banga Desire ,   Emmanuel Busha Tibasima3, Egesa Walufu Ivan , Odong Richard Justin ,   Grace Ndeezi1
Published: September 2024

Abstract

Introduction
Cerebral malaria, caused by Plasmodium falciparum, represents the most severe neurologic complication of malaria. Its association with high morbidity and mortality rates, especially among young children, underscores its clinical significance. In this study, we aimed to determine the prevalence of cerebral malaria and identify associated factors among children admitted with severe malaria at a tertiary hospital in western Uganda.
Methods
This was a cross-sectional, descriptive and analytical study involving children aged 6 to 59 months admitted with severe malaria. The study was conducted from January to March 2023 at Fort Portal Regional Referral Hospital. Severe and cerebral malaria were defined as per the WHO criteria. Sociodemographic, clinical and laboratory data were collected and analyzed using IBM SPSS version 27. Logistic regression analysis was used to evaluate the factors associated with cerebral malaria. A p value <0.05 indicated statistical significance.
Results
A total of 250 children were recruited (mean age 33.1±17.3 months). The prevalence of cerebral malaria was 12.8% (95% C. I: 8.9-17.6). Cerebral malaria was independently associated with male sex (aOR: 3.05, 95%C. I: 1.20-7.77, p=0.02), abnormal bleeding (aOR: 13.22, 95%C. I: 11.54-15.16, p=0.001), history of convulsions (aOR 12.20, 95% C. I:10.7, -21. 69, p= 0.010), acute kidney injury (aOR: 4.50, 95% C. I: 1.30-15.53, p=0.02), and hyponatremia (aOR: 3.47, 95% C. I: 1.34-8.96, p=0.010).
Conclusions and recommendations
The prevalence of cerebral malaria was high among children with severe malaria. Male sex, a history of convulsion, abnormal bleeding, acute kidney injury, and hyponatremia were associated with increased odds of cerebral malaria. These characteristics should be properly investigated and adequately treated in young children to reduce the risk and complications associated with cerebral malaria.
A Study of Prevalence of Gastroesophageal Reflux symptoms among Staff nurses involved in Night shifts in a Tertiary care hospital in Ariyur- A Cross Sectional Study.
Authors: Thiru Aravindan , kannan Suresh 
Published: September 2024

Abstract

Background Sleep deprivation may induce gastrointestinal disturbances and gastrointestinal symptoms may also lead to sleep derangements. Staff nurses in night shifts have high stress and burnouts.
Objectives 1. To assess the prevalence of gastrointestinal Reflux symptoms among staff nurses involved in night shifts in a tertiary care centre in Puducherry. 2. To find association between reflux symptoms and sleep quality among the staff nurses in night shift.
Material and methods The present study was cross sectional study carried out among staff nurses doing night shifts in a tertiary care centre in Puducherry for a period of 3 months. Participants with preexisting acid peptic disease, GI motility disorders, recent gastrointestinal surgeries, usage of analgesics at least once a month, pre-existing sleep disorders, pregnancy were excluded. The data was collected in a semi structured questionnaire. Statistical analysis was done using descriptive and analytical statistics.
Results Around 60% of the participants were found to have poor sleep quality. Around 14% had 89% chance of having GERD and 25% had 79% chance of GERD based on their symptoms. The regard to the association between GERD and sleep quality, those with poor sleep quality were found to have increased chance of experiencing GERD symptoms and the probability of GERD was also higher in them than those with good quality sleep according to PSQI.
Conclusion Both Gastroesophageal reflex disease and Sleep quality were found to be positively associated with each other.
Clinical Characteristics, Nutritional Status, and Disease Severity in Pediatric Patients with COVID-19
Authors: Mirkarimi Mohammadreza , Aminasnafi Ali , Alisamir Mohsen , Sabetsarvestani Fatemeh , Mohammadi Shooka 
Published: September 2024

Background:
Limited clinical data are available regarding the impact of coronavirus
disease 2019 (COVID-19) on children.

Objective:
This study assessed the clinical characteristics, nutritional status, and disease severity
in pediatric patients with COVID-19.

Methods:
A retrospective study examined medical records of children with COVID-19 admitted
to Abuzar Hospital (Ahvaz, Iran) for six months. Data related to their clinical, nutritional status
and demographic characteristics were documented.

Results:
This study included medical records of 500 patients. More than half of them were boys.
Different levels of COVID-19 severity were observed in 202, 172, and 126 children as mild, moderate,
and severe cases, respectively. Children with severe or moderate COVID-19 had substantially
elevated levels of various inflammatory markers, blood urea nitrogen (BUN), gamma-glutamyl
transferase (GGT), neutrophils, alanine transaminase (ALT), creatinine, bilirubin, and aspartate
aminotransferase (AST) compared to those with mild COVID-19 (p < 0.001). They had lower levels
of lymphocytes and vitamin D, as well as longer hospital stays than children with mild
COVID-19 (p < 0.001). In addition, 52% and 40.2% of patients had malnutrition and anemia, respectively.
The majority of underweight and stunted children had moderate or severe COVID-19.

Conclusion:
This study revealed a high prevalence of malnutrition, anemia, insufficient levels of
vitamin D, elevated levels of inflammatory markers, and abnormal liver and kidney function tests
in children with severe or moderate cases of COVID-19 compared to those with mild COVID-19.
Pengaruh Koneksi Politik dan Insentif Eksekutif Terhadap Praktik Penghindaran Pajak
Authors: Lase Maria Inggried Soinia , Subroto Bambang 
Published: September 2024

This research examined the effect of political connections and executive incentives on tax avoidance. The method used in this study is a descriptive-quantitative method with multiple linear regression tests for the analysis. The population of this study is all companies listed on the Indonesia Stock Exchange in 2020, with a sample consisting of 300 companies. This research finds that political connections increase tax avoidance practices, while executive incentives do not. These findings indicate that political connections make it easier for companies to access legislators, enabling companies to get information about state policies in advance and lowering the risk of being audited by the tax authorities. This research supports the rent-seeking theory so that this research can be used by the government, especially the tax authorities, as an evaluation material to periodically formulate regulations related to tax revenues and start paying special attention to the tax obligations of companies that have connections with the government. Furthermore, the results of this study suggest that future researchers should develop research by considering, developing, and examining other factors that may influence tax evasion by companies outside the variables in this study and extend the research period.
Abstrak
Penelitian ini dilaksanakan dengan tujuan untuk menguji pengaruh koneksi politik dan insentif eksekutif terhadap penghindaran pajak. Metode yang digunakan dalam penelitian ini adalah metode deskriptif-kuantitatif dengan uji regresi linear berganda untuk analisisnya. Adapun populasi penelitian ini adalah seluruh perusahaan yang terdaftar di Bursa Efek Indonesia tahun 2020 dengan sampel yang terdiri dari 300 perusahaan. Penelitian ini menemukan bahwa koneksi politik memicu terjadinya peningkatan praktik penghindaran pajak, sedangkan insentif eksekutif tidak. Temuan ini menunjukkan bahwa koneksi politik membuat perusahaan memiliki akses yang lebih mudah kepada legislator sehingga memungkinkan perusahaan mendapat informasi perihal kebijakan negara lebih dulu dan risiko perusahaan untuk diperiksa oleh otoritas pajak menjadi lebih rendah. Penelitian ini mendukung teori rent-seeking sehingga penelitian ini dapat digunakan oleh pemerintah, terkhusus otoritas pajak, sebagai bahan evaluasi untuk secara berkala menyusun regulasi yang berhubungan dengan penerimaan perpajakan serta mulai memberikan perhatian khusus terhadap kewajiban perpajakan perusahaan-perusahaan yang memiliki koneksi dengan pemerintahan. Selanjutnya, hasil dari penelitian ini menyarankan peneliti selanjutnya untuk mengembangkan penelitian dengan mempertimbangkan, mengembangkan, dan meneliti faktor lain yang dapat memengaruhi penghindaran pajak yang dilakukan oleh perusahaan di luar variabel dalam penelitian ini dan memperpanjang periode penelitian.
Central Hemodynamic and Thermoregulatory Responses to Food Intake as Potential Biomarkers for Eating Detection: Systematic Review
Authors: Chikwetu Lucy , Vakili Parker , Takais Andrew , Younes Rabih 
Published: September 2024

Background
Diet-related diseases, such as type 2 diabetes, require strict dietary management to slow down disease progression and call for innovative management strategies. Conventional diet monitoring places a significant memory burden on patients, who may not accurately remember details of their meals and thus frequently falls short in preventing disease progression. Recent advances in sensor and computational technologies have sparked interest in developing eating detection platforms.

Objective
This review investigates central hemodynamic and thermoregulatory responses as potential biomarkers for eating detection.

Methods
We searched peer-reviewed literature indexed in PubMed, Web of Science, and Scopus on June 20, 2022, with no date limits. We also conducted manual searches in the same databases until April 21, 2024. We included English-language papers demonstrating the impact of eating on central hemodynamics and thermoregulation in healthy individuals. To evaluate the overall study quality and assess the risk of bias, we designed a customized tool inspired by the Cochrane assessment framework. This tool has 4 categories: high, medium, low, and very low. A total of 2 independent reviewers conducted title and abstract screening, full-text review, and study quality and risk of bias analysis. In instances of disagreement between the 2 reviewers, a third reviewer served as an adjudicator.

Results
Our search retrieved 11,450 studies, and 25 met our inclusion criteria. Among the 25 included studies, 32% (8/25) were classified as high quality, 52% (13/25) as medium quality, and 16% (4/25) as low quality. Furthermore, we found no evidence of publication bias in any of the included studies. A consistent postprandial increase in heart rate, cardiac output, and stroke volume was observed in at least 95% (heart rate: 19/19, cardiac output: 18/19, stroke volume: 11/11) of the studies that investigated these variables’ responses to eating. Specifically, cardiac output increased by 9%-100%, stroke volume by 18%-41%, and heart rate by 6%-21% across these studies. These changes were statistically significant (P<.05). In contrast, the 8 studies that investigated postprandial thermoregulatory effects displayed grossly inconsistent results, showing wide variations in response with no clear patterns of change, indicating a high degree of variability among these studies.

Conclusions
Our findings demonstrate that central hemodynamic responses, particularly heart rate, hold promise for wearable-based eating detection, as cardiac output and stroke volume cannot be measured by any currently available noninvasive medical or consumer-grade wearables.

Trial Registration
PROSPERO CRD42022360600; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=360600