bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Polymorphisms of Alcohol Metabolizing Enzymes and Alcohol Consumption are Associated With Asymptomatic Cardiac Remodeling and Subclinical Systolic Dysfunction in Large Community-Dwelling Asians
Authors: Hung Chung-Lieh , Chang Shun-Chuan , Chang Sheng-Hsiung , Chi Po-Ching , Lai Yu-Jun , Wang Shih-Wei , Wu Yih-Jer , Yeh Hung-I , Lin Shing-Jong , Chen Che-Hong , Mochly-Rosen Daria , Wang Li-Yu ,   for the MAGNET Study Investigator
Published: July 2017

Abstract

Aims
Excessive consumption of alcoholic beverages is associated with cardiac remodeling and cardiomyopathy. We examined the possible association of alcohol use, common Asian genetic variants in genes involved in alcohol metabolism, and cardiac structures/functions alterations.

Methods
A prospective, community-dwelling survey among individuals with available complete echocardiography examined the associations of alcohol use, cardiac structure/functions, and three common alcohol metabolizing genetic variants, including aldehyde dehydrogenase 2 (ALDH2), alcohol dehydrogenase 1B (ADH1B) and cytochrome P450 (CYP) isoform 2E1 (CYP2E1).

Results
Among 1577 participants (mean age: 53 ± 9, 59.7% female), we observed that in subjects with more frequent weekly ethanol intake showed greater left ventricle (LV) mass, more impaired diastolic functions, and reduced global longitudinal strain (GLS), systolic (SRs) and early diastolic strain rates (SRe) (P<0.05). After propensity matching for clinical confounders (n = 330:30 for frequent users and non-users), frequent alcohol use and subjects carrying ALDH2 (A/G or A/A), ADH1B (A/A) or CYP2E1(T/C or T/T) polymorphisms were all associated with worse GLSRs and GLSRe, with combined alcohol use and any given genetic variant aggravated these associations (all P < 0.05). Finally, we observed Gene–Gene synergistic effects on LV functional decline in frequent alcohol users by using linear mixed effect model (all interaction P < 0.05).

Conclusions
Among East Asians, even moderate alcohol consumption can confer subclinical adverse effects on cardiac systolic functions, which was most pronounced in subjects carrying common variants in alcohol metabolizing genes. These findings challenge the notion of beneficial influences of less heavy ethanol consumption on the heart, especially among East Asians.

Short summary
This study evaluated the association of level of alcohol consumption and genetic variants in genes involved in alcohol metabolism with changes in cardiac function in East Asians. Even moderate alcohol use conferred subclinical adverse effects on cardiac systolic functions, which were most pronounced in subjects carrying common alcohol metabolizing genes.
A Pilot Study: The UNC Passive Aerosol Sampler in a Working Environment
Authors: Shirdel Mariam , Wingfors Håkan , Andersson Britt M , Sommar Johan N , Bergdahl Ingvar A , Liljelind Ingrid E 
Published: July 2017

Abstract

Objectives
Dust is generally sampled on a filter using air pumps, but passive sampling could be a cost-effective alternative. One promising passive sampler is the University of North Carolina passive aerosol sampler (UNC sampler). The aim of this study is to characterize and compare the UNC sampler’s performance with PM10 and PM2.5 impactors in a working environment.

Methods
Area sampling was carried out at different mining locations using UNC samplers in parallel with PM2.5 and PM10 impactors. Two different collection surfaces, polycarbonate (PC) and carbon tabs (CT), were employed for the UNC sampling. Sampling was carried out for 4–25 hours.

Results
The UNC samplers underestimated the concentrations compared to PM10 and PM2.5 impactor data. At the location with the highest aerosol concentration, the time-averaged mean of PC showed 24% and CT 35% of the impactor result for PM2.5. For PM10, it was 39% with PC and 58% with CT. Sample blank values differed between PC and CT. For PM2.5, PC blank values were ~7 times higher than those of CT, but only 1.8 times higher for PM10. The blank variations were larger for PC than for CT.

Conclusions
Particle mass concentrations appear to be underestimated by the UNC sampler compared to impactors, more so for PM2.5 than for PM10. CT may be preferred as a collection surface because the blank values were lower and less variable than for PC. Future validations in the working environment should include respirable dust sampling.
Neuronal Expression of Opioid Gene is Controlled by Dual Epigenetic and Transcriptional Mechanism in Human Brain
Authors: Bazov Igor , Sarkisyan Daniil , Kononenko Olga , Watanabe Hiroyuki , Taqi Mumtaz Malik , Stålhandske Lada , Verbeek Dineke S , Mulder Jan , Rajkowska Grazyna , Sheedy Donna , Kril Jillian , Sun Xueguang , Syvänen Ann-Christine , Yakovleva Tatiana , Bakalkin Georgy 
Published: July 2017

AbstractMolecular mechanisms that define patterns of neuropeptide expression are essential for the formation and rewiring of neural circuits. The prodynorphin gene (PDYN) gives rise to dynorphin opioid peptides mediating depression and substance dependence. We here demonstrated that PDYN is expressed in neurons in human dorsolateral prefrontal cortex (dlPFC), and identified neuronal differentially methylated region in PDYN locus framed by CCCTC-binding factor binding sites. A short, nucleosome size human-specific promoter CpG island (CGI), a core of this region may serve as a regulatory module, which is hypomethylated in neurons, enriched in 5-hydroxymethylcytosine, and targeted by USF2, a methylation-sensitive E-box transcription factor (TF). USF2 activates PDYN transcription in model systems, and binds to nonmethylated CGI in dlPFC. USF2 and PDYN expression is correlated, and USF2 and PDYN proteins are co-localized in dlPFC. Segregation of activatory TF and repressive CGI methylation may ensure contrasting PDYN expression in neurons and glia in human brain.
A Long-Term Study of the Treatment of Nelson's Syndrome With Gamma Knife Radiosurgery
Authors: Caruso James P , Patibandla Mohana Rao , Xu Zhiyuan , Vance Mary Lee , Sheehan Jason P 
Published: July 2017

Abstract

BACKGROUND
Nelson's syndrome may be a devastating complication for patients with Cushing's disease who underwent a bilateral adrenalectomy. Previous studies have demonstrated that stereotactic radiosurgery (SRS) can be used to treat patients with Nelson's syndrome.

OBJECTIVE
To report a retrospective study of patients with Nelson's syndrome treated with Gamma Knife radiosurgery to evaluate the effect of SRS on endocrine remission and tumor control.

METHODS
Twenty-seven patients with Nelson's syndrome treated with Gamma Knife radiosurgery after bilateral adrenalectomy were included in this study. After radiosurgery, patients were followed with serial adrenocorticotropic hormone (ACTH) levels and MRI sequences to assess for endocrine remission and tumor control. Cox proportional hazards regression analysis was used to evaluate the relationship between the time to remission and potential prognostic factors.

RESULTS
In 21 patients with elevated ACTH prior to SRS and endocrine follow-up data, 14 (67%) had decreased or stable ACTH levels, and 7 achieved a normal ACTH level at a median of 115 mo (range 7-272) post-SRS. Tumor volume was stable or reduced after SRS in 92.5% of patients (25/27) with radiological follow-up. Time to remission was not significantly associated with the ACTH prior to SRS (P = .252) or with the margin dose (P = .3). However, a shorter duration between the patient's immediate prior transsphenoidal resection and SRS was significantly associated with a shorter time to remission (P = .045).

CONCLUSION
This retrospective analysis suggests that SRS is an effective means of achieving endocrine remission and tumor control in patients with Nelson's syndrome.
Postoperative Pyoderma Gangrenosum in Children: The Case Report of a 13-Year-Old Boy With Pyoderma Gangrenosum After Hip Reconstruction Surgery and a Review of the Literature
Authors: Berger Nina , Ebenhoch Michael , Salzmann Maya 
Published: July 2017

Background:
Postoperative pyoderma gangrenosum (PG) is a neutrophilic dermatosis and pathergic reaction at surgical sites. Reports of children with postoperative PG are rare in the literature.

Methods:
We report the case of a 13-year-old boy without any known preexisting illnesses, who developed severe systemic response and wound deterioration after elective hip reconstruction surgery. The working diagnosis of necrotizing fasciitis was later determined to be postoperative PG.

Results:
The patient was successfully treated by the systemic application of corticosteroids.

Conclusions:
Postoperative PG is a very rare complication after surgery, especially in children. If mistaken for necrotizing fasciitis, it can substantially mutilate a patient because PG is worsened by surgical debridement. It can be treated successfully only by corticosteroids and other immunosuppressive drugs.

Level of Evidence:
Level IV—this is a case report.
Suppression of Sympathetic Nerve Sprouting by Local Administration of an α-antagonist Around the Dorsal Root Ganglion in a Lumbar Radiculopathy Model
Authors: Ogon Izaya , Takebayashi Tsuneo , Miyakawa Tsuyoshi , Iwase Takehito , Tanimoto Katsumasa , Terashima Yoshinori , Jimbo Syunsuke , Kobayashi Takeshi , Tohse Noritsugu , Yamashita Toshihiko 
Published: July 2017

Study Design.
Animal experimental study with intervention.

Objective.
The purpose of this study was to elucidate whether local administration of an α-antagonist around the dorsal root ganglion (DRG) suppressed sympathetic nerve sprouting, from the acute to the chronic pain development phase, in a lumbar radiculopathy model using immunohistochemical methods.

Summary of Background Data.
The abnormal sympathetic-somatosensory interaction may underlie some forms of neuropathic pain. There were several reports suggesting α-antagonists are effective to treat neuropathic pain. However, its pathophysiological mechanisms remain obscure.

Methods.
We used 70 male Sprague-Dawley rats. After root constriction (RC), rats received a series of three local injections of the nonselective α-antagonist phentolamine around the DRG for 3 days. There were three groups of rats: those that were injected from the day of surgery and those injected from day 4 and third group injected from day 11. The control rats were subjected to RC but equal-volume normal saline injections, and the naïve rats were not subjected to any surgical procedures. At the 14th postoperative day, the left L5 DRG was removed, embedded in paraffin, and sectioned. Sections were then immunostained with antibodies to tyrosine hydroxylase (TH). To quantify the extent of the presence of sympathetic nerve fibers, we counted TH-immunoreactive fibers in the DRG using a light microscope equipped with a micrometer graticule. We counted the squares of the graticule, which contained TH-immunoreactive fibers for each of five randomly selected sections of the DRG.

Results.
In the naïve group, TH-immunoreactive fibers were scarce in the DRG. α-antagonist injections from postoperative day 0 and 4 suppressed sympathetic nerve sprouting compared with the control group. α-antagonist injections from postoperative day 11 had no suppressant effect compared with the control group.

Conclusion.
The α-antagonist administered around the DRG could suppress neural plastic changes in the early phase after nerve injury.

Level of Evidence: N/A
Does an Annular Puncture Influence the Herniation Path?
Authors: van Heeswijk Vonne M. , Thambyah Ashvin , Robertson Peter A. , Broom Neil D. 
Published: July 2017

Study Design.
A study of mechanically induced herniation in punctured ovine discs followed by structural analysis.

Objective.
To investigate whether an annular puncture influences the path that herniation takes by providing direct passage for nucleus through the annulus and therefore whether it increases the risk of acute herniation from overload at the site of damage independent of any longer-term degeneration.

Summary of Background Data.
Ten years after treatment with discography both degenerative changes and frequency of herniation have been shown to increase compared to untreated discs. Although the effect of an annular puncture over time has been widely investigated the question of whether it increases the risk of acute herniation has not been resolved.

Methods.
The posterolateral annuli of healthy ovine lumbar discs were punctured with either a 25-gauge (n = 8) or a larger 18-gauge (n = 8) needle and then compressed in a flexed posture of 10° until initial indications of failure. The entire volume of the disc was visually assessed for structural damage by obtaining progressive, full transverse cross-sections of its entire height thus exposing all regions of the disc.

Results.
There was no association between the 25-gauge puncture and disc disruption and herniation. In contrast, nuclear material was observed to migrate through the 18-gauge needle puncture. Disruption of the lateral inner annulus was observed in 12 out of the 16 discs tested.

Conclusion.
The risk of acute herniation through the puncture site is dependent on the needle diameter used. Under the conditions employed the lateral inner annulus remains the site most vulnerable to disruption independent of the presence of a posterolateral puncture.

Level of Evidence: N /A
Comparative Analysis of Chilblain Lupus Erythematosus and Idiopathic Perniosis: Histopathologic Features and Immunohistochemistry for CD123 and CD30
Authors: Wang Michael L. , Chan May P. 
Published: July 2017

Abstract:
Distinction of chilblain lupus erythematosus (CLE) from idiopathic perniosis (IP) could predict an underlying connective tissue disease; however, histopathologic discrimination of the two is difficult. Increased CD123+ plasmacytoid dendritic cells and CD30+ lymphocytes have been demonstrated in various forms of cutaneous lupus erythematosus and IP, respectively. To our knowledge, CD123 and CD30 have not been examined in CLE. Our objective was to identify helpful histopathologic and immunohistochemical features in distinguishing CLE and IP. Skin biopsies classified as CLE (n = 20) and IP (n = 39) based on clinicopathologic correlation were collected from 2000 to 2015. Various histopathologic features were examined on hematoxylin and eosin and alcian blue stains. CD123 and CD30 immunostains were performed and characterized. We identified dermal interstitial fibrin exudate (P = 0.0352) and increased dermal mucin (P = 0.0002) as features significantly associated with CLE. Other histopathologic features and CD123 failed to distinguish between groups. CD30+ lymphocytes were sparse in all cases. Despite being the largest series of CLE and IP to date, the number of CLE cases in this study remained relatively limited, and some patients in the IP group may have yet to develop diagnostic features of systemic lupus erythematosus. In conclusion, histopathologic distinction between CLE and IP remains challenging. Interstitial fibrin and abundant dermal mucin help favor CLE. The number and distribution of CD123+ plasmacytoid dendritic cells and CD30+ lymphocytes have no discriminatory role.
Restrictions and limitations after pelvic floor surgery: what's the evidence?
Authors: Murphy Miles 
Published: July 2017

Purpose of review
A common concern regarding pelvic floor surgery is the relatively high risk of recurrence. In an effort to minimize this risk, many surgeons instruct their patients to avoid certain activities during the healing process so as not to damage the repair before proper healing has occurred. However, many of these restrictions have been historically based on little to no hard evidence. The purpose of this review is to present the latest evidence-based recommendations regarding restrictions and limitations after pelvic floor surgery.

Recent findings
The current review covers postoperative activities that could impact proper healing of a vaginal incision and of the strength of the reconstruction itself. It also looks at safety of the patient and those around her as she heals. Topics include pelvic rest, swimming, lifting exercising, working, and driving. Observational research suggests that many unavoidable activities of daily living may have as great, if not an even greater, risk of impacting the healing process than many of the modifiable activity restrictions that are commonly imposed on patients. This may explain why recent clinical trials show no greater problem with healing in patients randomized to less strict postoperative restrictions than the standard.

Summary
Although further research is necessary, it appears that patients are more satisfied with less strict postoperative limitations, and this less restrictive activity may not have any significant negative impact on the healing process.
Vent Incisions to Facilitate Peripheral Unfolding of the DMEK Graft
Authors: Liu Christopher , Vasquez-Perez Alfonso , Chervenkoff Jordan Vassileff , Avadhanam Venkata 
Published: July 2017

Purpose:
To present the results of 15 cases in which a modified technique of Descemet membrane endothelial keratoplasty (DMEK) with vent incisions was used to assist unfolding of tight peripheral inward folds.

Methods:
This is a retrospective case series of 15 consecutive eyes that underwent a modified vent incision technique for DMEK. Six oblique, small-size (1 mm) vent incisions parallel to the limbus and 3.5 mm from the center were performed after descemetorhexis. The graft was opened with standard no-touch techniques and was partially attached to the stroma with air injection. Then, in cases with remaining tight inward folds despite gentle corneal tapping, jets of balanced salt solution were pulsed through the vent incisions directed at the peripheral folds to achieve complete unfolding.

Results:
Descemet membrane endothelial keratoplasty was performed in 2 phakic eyes (14%) and 13 pseudophakic eyes (86%). Six cases (40%) required fluid injection through the vent incisions: 3 corresponded to grafts from younger donors (54, 41, and 29 years old), and 3 had a 9.0-mm-sized graft. Complete graft unfolding was successfully achieved in 14 cases (93%), and in 1 case (7%), peripheral folds remained because of spontaneous bleeding in the anterior chamber. A clear cornea was achieved in all cases, and there were no postoperative complications associated with the vent incisions.

Conclusions:
This technique provides a different DMEK no-touch approach that could be used in challenging cases of graft unfolding such as young donors and/or large-sized grafts. It may also avoid excessive unfolding times and direct manipulation that are related to higher endothelial cell loss.
Comparison of Glaucoma Progression Between Unilateral and Bilateral Disc Hemorrhage Eyes and Associated Risk Factors for Progression
Authors: Seol Bo Ram , Kim Young Kook , Jeoung Jin Wook , Park Ki Ho 
Published: July 2017

Purpose:
To compare glaucoma progression between unilateral and bilateral disc hemorrhage (DH) eyes and to determine the associated risk factors.

Methods:
A total of 78 bilateral primary open-angle glaucoma (POAG) patients who had shown one or more occurrences of DH (48 eyes of 38 unilateral DH patients and 30 eyes of 30 bilateral DH patients) were included. Glaucoma progression was defined by visual field (VF) deterioration. The mean VF global indices progression rates in the pre-DH, post-DH and total follow-up periods were compared between the unilateral and bilateral DH groups. The cumulative probability of glaucoma progression between the 2 groups was compared by the Kaplan-Meier survival analysis. Univariate and multivariate Cox proportional hazard models were used to identify the risk factors for glaucoma progression.

Results:
Among the mean VF global indices progression rates, only the mean pattern standard deviation progression rate showed a statistically significant difference between the groups (unilateral DH group: 0.30±0.38 dB/y; bilateral DH group: 0.13±0.27 dB/y) (P=0.042). The Kaplan-Meier analysis revealed a greater cumulative probability of progression in the unilateral than in the bilateral DH group (P=0.008). The multivariate Cox’s proportional hazard model indicated unilaterality of DH to be a factor associated with glaucoma progression (hazard ratio, 2.587; P=0.030).

Conclusions:
In bilateral POAG patients with DH, unilaterality of DH is associated with glaucoma progression. Therefore, clinicians need to perform careful and frequent follow-up on bilateral POAG patients showing DH in only 1 eye during the follow-up period.
Predictive Factors for Hearing Outcomes After Canaloplasty in Patients With Congenital Aural Atresia
Authors: Ahn Jungmin , Baek Sun Young , Kim Kyunga , Cho Yang-Sun 
Published: July 2017

Objective:
The aim of this study was to determine the most important factors in preoperative imaging, including components of Jahrsdoefer score (J score), and favorable prognostic factors for postoperative hearing results after canaloplasty for congenital aural atresia.

Study Design:
Retrospective review of medical records.

Setting, Patients, Intervention, Main Outcome Measure:
One hundred eight patients who underwent canaloplasty by a single surgeon between January 2011 and July 2014 were included. The influence of the following factors on the success for restoring of hearing was evaluated by univariable and multivariable logistic regression analyses: sex, atretic side, total J score, each component of Jahrsdoerfer grading scale, incudostapedial joint angle, inferior displacement of the tegmen, middle ear height, and inner ear anomaly.

Results:
Successful hearing outcome was defined as postoperative air conduction ≤40 dB at 3 months after surgery, and it was achieved in 74 of 108 ears (70.5%). Univariable analysis revealed that the total J score, course of the facial nerve, presence of the malleus-incus complex, pneumatization of the mastoid, and middle ear height were significant as favorable predictive factors. Multivariable analysis revealed that middle ear height and inferior displacement of the tegmen were significant as favorable predictive factors. Inferior displacement of the tegmen was the most significant factor by the stepwise selection method in the final model.

Conclusion:
Middle ear height and inferior displacement of the tegmen are useful factors in predicting favorable hearing results after canaloplasty for congenital aural atresia. These factors might have a useful prognostic value supplementary to the J score.
Use of Colorectal Cancer Screening Among People With Mobility Disability
Authors: Gofine Miriam , Mielenz Thelma J. , Vasan Sowmya , Lebwohl Benjamin 
Published: July 2017

Goals:
We aimed to assess use of colorectal cancer screening (CRCS) as per United States Preventive Task Force guidelines among people with mobility disability using a nationally representative data set.

Background:
Individuals with mobility disability have decreased access to health care services, but the impact of mobility disability on CRCS has not been investigated.

Study:
Data from the 2013 National Health Interview Survey were used to estimate sociodemographic characteristics of adults with mobility disability, prevalence of CRCS, and odds of CRCS given mobility disability among Americans aged 50 to 75.

Results:
In total, 56.8% of the entire sample (n=81,953,585) were up-to-date with CRCS. Mobility disability was not associated with CRCS status on univariable analysis but was significantly associated after adjustment for covariates including age and comorbidities, with an inverse relationship between the degree of mobility disability and odds of CRCS. Odds ratio for CRCS given progressively severe disability were 0.78 (0.66 to 0.93), 0.71 (0.53 to 0.94), 0.65 (0.31 to 1.19).

Conclusions:
The present study indicates reduced CRCS among people with mobility disability and highlights the need for CRCS to be especially targeted toward this group. Future research should identify the specific systemic, social, and/or physical barriers to CRCS for this subgroup so that they can be addressed.