bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eliminating the blood‐flow confounding effect in intravoxel incoherent motion (IVIM) using the non‐negative least square analysis in liver
Authors: Gambarota Giulio , Hitti Eric , Leporq Benjamin , Saint‐Jalmes Hervé , Beuf Olivier 
Published: January 2016

PurposeTissue perfusion measurements using intravoxel incoherent motion (IVIM) diffusion‐MRI are of interest for investigations of liver pathologies. A confounding factor in the perfusion quantification is the partial volume between liver tissue and large blood vessels. The aim of this study was to assess and correct for this partial volume effect in the estimation of the perfusion fraction.MethodsMRI experiments were performed at 3 Tesla with a diffusion‐MRI sequence at 12 b‐values. Diffusion signal decays in liver were analyzed using the non‐negative least square (NNLS) method and the biexponential fitting approach.ResultsIn some voxels, the NNLS analysis yielded a very fast‐decaying component that was assigned to partial volume with the blood flowing in large vessels. Partial volume correction was performed by biexponential curve fitting, where the first data point (b = 0 s/mm2) was eliminated in voxels with a very fast‐decaying component. Biexponential fitting with partial volume correction yielded parametric maps with perfusion fraction values smaller than biexponential fitting without partial volume correction.ConclusionThe results of the current study indicate that the NNLS analysis in combination with biexponential curve fitting allows to correct for partial volume effects originating from blood flow in IVIM perfusion fraction measurements. Magn Reson Med 77:310–317, 2017. © 2016 Wiley Periodicals, Inc.
Thai Elephant‐Assisted Therapy Programme in Children with Down Syndrome
Authors: Satiansukpong Nuntanee , Pongsaksri Maethisa , Sasat Daranee 
Published: January 2016

AbstractThe objectives of this study were to examine the effects of the Thai Elephant‐Assisted Therapy Programme for children with Down syndrome (DS) (TETP‐D) on balance, postural control and visual motor integration (VMI). A quasi‐experimental design with blind control was used. Sixteen children with DS from grades 1 to 6, in a Thailand, public school were recruited for this study. The participants were divided voluntarily into two groups: control and experimental. These both groups received regular school activities, but the experimental group had added treatment, which consisted of TETP‐D twice a week for 2 months. The balance subtest of the Bruininks–Oseretsky Test of Motor Proficiency 2, the postural control record form and Beery VMI were applied as outcome measure 1 week before and after the TETP‐D. The results showed no significant difference in balance or postural control. However, a significant difference of VMI was shown between the two groups (z = 13.5, p = .04). Children with DS benefited from the TETP‐D as it improved their VMI. The TETP‐D could improve balance and postural control if provided within a suitable frequency and duration. Further research is needed to test this hypothesis. The limitations of this study are the significant differences in some aspects of the groups at pre‐test such as gender and supine flexion of postural control. Copyright © 2015 John Wiley & Sons, Ltd.
Emulsion copolymerization of substituted acetylenes for constructing optically active helical polymer nanoparticles. Synergistic effects and helicity inversion
Authors: Zhao Biao , Deng Jianping , Yang Wantai 
Published: January 2016

ABSTRACTCopolymerizations of substituted acetylenes have been intensively studied in solution polymerization for constructing chirally helical polymers, while emulsion copolymerizations of the kind of monomers have been only scarcely reported. In the present study, chiral substituted acetylene monomer containing cholic acid group underwent emulsion copolymerizations with an achiral acetylenic monomer in the presence of rhodium catalyst, providing optically active helical copolymer nanoparticles. Synergistic effects were found in the resulting helical copolymers, enabling one certain copolymer to show the maximum CD signal intensity. Moreover, the helicity of the helical copolymers in nanoparticle state was opposite to that in solution state. This is the first demonstration that synergistic effects and helicity inversion simultaneously occurred in helical copolymers prepared by emulsion copolymerization process. To deepen the understanding of the unique phenomena, corresponding solution copolymerization and emulsification process were also investigated. Different from “Sergeant and Soldiers rule” approach, the present study provides a new strategy for preparing chirally helical polymer particles by making full use of achiral monomers. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 1679–1685
Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe3+ sensing
Authors: Yang Dongliang , Li Fei , Luo Zhimin , Bao Biqing , Hu Yanling , Weng Lixing , Cheng Yixiang , Wang Lianhui 
Published: January 2016

ABSTRACTIn this article, a novel zwitterionic conjugated polyelectrolyte containing tetraphenylethene unit was synthesized via Pd‐catalyzed Sonogashira reaction. The resulting polymer (P2), which exhibited typical aggregation‐induced emission (AIE) properties, was weakly fluorescent in dilute DMSO solution and showed bright fluorescence emissions when aggregated in DMSO/water mixtures or fabricated into conjugated polymer nanoparticles (CPNs). The nanoparticles from P2 could be prepared by reprecipitation method with an average diameter around 23 nm. Notably, the cell‐staining efficiencies of lipid‐P2 nanoparticles could be enhanced with lipid encapsulation and these nanoparticles were endocytosed via caveolae‐mediated and clathrin‐mediated endocytosis pathways. Furthermore, the lipid‐P2 nanoparticles with low cytotoxicity, high photostability and efficient cell staining ability could be employed for in vitro detection of Fe3+ ions in A549 cells. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 1686–1693
Development of laser ablation multi‐collector inductively coupled plasma mass spectrometry for boron isotopic measurement in marine biocarbonates: new improvements and application to a modern Porites coral
Authors: Thil François , Blamart Dominique , Assailly Caroline , Lazareth Claire E. , Leblanc Thierry , Butsher John , Douville Eric 
Published: January 2016

RationaleLaser Ablation coupled to Multi‐Collector Inductively Coupled Plasma Mass Spectrometry (LA‐MC‐ICPMS) is a powerful tool for the high‐precision measurement of the isotopic ratios of many elements in geological samples, with the isotope ratio (11B/10B) of boron being used as an indicator of the pH of oceanic waters. Most geological samples or standards are polished and ablation occurs on flat surfaces. However, the shape and the irregularities of marine biocarbonates (e.g., corals, foraminifera) can make precise isotopic measurements of boron difficult. Even after polishing, the porosity properties and the presence of holes or micro‐fractures affect the signal and the isotopic ratio when ablating the material, especially in raster mode.MethodsThe effect of porosity and of the crater itself on the 11B signal and the isotopic ratio acquired by LA‐MC‐ICPMS in both raster and spot mode was studied. Characterization of the craters was then performed with an optical profilometer to determine their shapes and depths. Surface state effects were examined by analyzing the isotopic fractionation of boron in silicate (NIST‐SRM 612 and 610 standards) and in carbonate (corals).ResultsSurface irregularities led to a considerable loss of signal when the crater depth exceeded 20 µm. The stability and precision were degraded when ablation occurred in a deep cavity. The effect of laser focusing and of blank correction was also highlighted and our observations indicate that the accuracy of the boron isotopic ratio does not depend on the shape of the surface. After validation of the analytical protocol for boron isotopes, a raster application on a Porites coral, which grew for 18 months in an aquarium after field sampling, was carried out.ConclusionsThis original LA‐MC‐ICPMS study revealed a well‐marked boron isotope ratio temporal variability, probably related to growth rate and density changes, irrespective of the pH of the surrounding seawater. Copyright © 2015 John Wiley & Sons, Ltd.
Die Bemessung vorwiegend ruhend beanspruchter Schweißverbindungen von Aluminiumtragwerken nach DIN EN 1999‐1‐1
Authors: Saal Helmut , Gitter Reinhold , Fellhauer Andreas 
Published: January 2016

AbstractSowohl die Bemessung von Aluminiumtragwerken nach DIN EN 1999‐1‐1 [1] im Allgemeinen als auch die der Schweißverbindungen im Besonderen unterscheidet sich von der nach DIN 4113 in vielerlei Grundsätzen. Bestehende deutsche Regelungen und aus der Anwendung der EN 1999‐1‐1 deutlich gewordene Lücken erforderten, dass durch ergänzende, nicht widersprechende Angaben, die so genannten NCI‐Regelungen, im Nationalen Anhang und seinen Änderungen Bemessungsregeln und Erläuterungen ergänzt wurden. Bei NCI‐Regelungen ist zu beachten, dass diese nur für Tragwerke und tragende Bauteile angewendet werden dürfen, die im betreffenden Land – das heißt also hier in Deutschland – zum Einsatz kommen. Da der Umfang der zerstörungsfreien Prüfung der Schweißnähte gemäß DIN EN 1090‐3 [5] vom Ausnutzungsgrad (Ausnutzungsklasse) und der Art des Bauwerks (Ausführungsklasse) abhängt und so indirekt die Beanspruchbarkeit bestimmt, werden nach der Behandlung der Bemessungsregeln für Schweißverbindungen abschließend die Regelungen der DIN EN 1090‐3 zur Ausführung und Prüfung von Schweißnähten beschrieben, die auch der Tragwerksplaner kennen muss.Design of predominantly statically loaded welded connections of aluminium structures according to DIN EN 1999‐1‐1. The design of aluminium structures according to DIN EN 1999‐1‐1 is different with respect to many design principles of DIN 4113 in general as well as especially for welded connections. Non‐contradicting existing German design rules and deficiencies (missing design provisions) which became obvious with the application of DIN EN 1999‐1‐1 in daily practice required and will require NCI‐regulations with supplemental design rules and comments in the National Annex and its amendments. The regulations of DIN EN 1090‐3 [5] for execution and testing of welds are discussed in connection with the design rules. This is important since the degree of nondestructive testing of welds according to DIN EN 1090‐3 [5] depends on the utilization factor (utilization range) and the nature of structure (execution class). Thus it indirectly determines the design resistance which is needed by the designing engineer.
Coronary Computed Tomography Angiography Derived Fractional Flow Reserve and Plaque Stress
Authors: Nørgaard Bjarne Linde , Leipsic Jonathon , Koo Bon-Kwon , Zarins Christopher K. , Jensen Jesper Møller , Sand Niels Peter , Taylor Charles A. 
Published: January 2016

Abstract
Fractional flow reserve (FFR) measured during invasive coronary angiography is an independent prognosticator in patients with coronary artery disease and the gold standard for decision making in coronary revascularization. The integration of computational fluid dynamics and quantitative anatomic and physiologic modeling now enables simulation of patient-specific hemodynamic parameters including blood velocity, pressure, pressure gradients, and FFR from standard acquired coronary computed tomography (CT) datasets. In this review article, we describe the potential impact on clinical practice and the science behind noninvasive coronary computed tomography (CT) angiography derived fractional flow reserve (FFRCT) as well as future applications of this technology in treatment planning and quantifying forces on atherosclerotic plaques.
One Asia, or Many? Reflections from connected history
Authors: SUBRAHMANYAM SANJAY 
Published: January 2016

It is now widely rumoured that the ‘Asian century’ is upon us. But what does this really mean? As late as 1988, Deng Xiaoping—in remarks made before the Indian prime minister, Rajiv Gandhi—expressed some scepticism about the facility of the formulation. As Deng stated then:In recent years people have been saying that the next century will be the century of Asia and the Pacific, as if that were sure to be the case. I disagree with this view. If we exclude the United States, the only countries in the Asia-Pacific region that are relatively developed are Japan, the ‘four little dragons’, Australia and New Zealand, with a total population of at most 200 million. (. . .) But the population of China and India adds up to 1.8 billion. Unless those two countries are developed, there will be no Asian century. No genuine Asia-Pacific century or Asian century can come until China, India and other neighbouring countries are developed. By the same token, there could be no Latin-American century without a developed Brazil. We should therefore regard the problem of development as one that concerns all mankind and study and solve it on that level. Only thus will we recognize that it is the responsibility not just of the developing countries but also of the developed countries.Whatever the doubts about his standing as a Marxist, then, we may say that Deng remained resolutely universalist in his perspective, at least outwardly.
Benjamin Lee Preston Receives 2015 Charles S. Falkenberg Award
Authors:   AGU
Published: January 2016

Benjamin Lee Preston received the 2015 Charles S. Falkenberg Award at the American Geophysical Union Fall Meeting Honors Ceremony, held on 16 December 2015 in San Francisco, Calif. The award honors an "early- to middle- career scientist who has contributed to the quality of life, economic opportunities, and stewardship of the planet through the use of Earth science information and to the public awareness of the importance of understanding our planet."
Crystal structure of E. coli lipoprotein diacylglyceryl transferase
Authors: Mao Guotao , Zhao Yan , Kang Xusheng , Li Zhijie , Zhang Yan , Wang Xianping , Sun Fei , Sankaran Krishnan , Zhang Xuejun C. 
Published: January 2016

AbstractLipoprotein biogenesis is essential for bacterial survival. Phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (Lgt) is an integral membrane enzyme that catalyses the first reaction of the three-step post-translational lipid modification. Deletion of the lgt gene is lethal to most Gram-negative bacteria. Here we present the crystal structures of Escherichia coli Lgt in complex with phosphatidylglycerol and the inhibitor palmitic acid at 1.9 and 1.6 Å resolution, respectively. The structures reveal the presence of two binding sites and support the previously reported structure–function relationships of Lgt. Complementation results of lgt-knockout cells with different mutant Lgt variants revealed critical residues, including Arg143 and Arg239, that are essential for diacylglyceryl transfer. Using a GFP-based in vitro assay, we correlated the activities of Lgt with structural observations. Together, the structural and biochemical data support a mechanism whereby substrate and product, lipid-modified lipobox-containing peptide, enter and leave the enzyme laterally relative to the lipid bilayer.
The KDM3A–KLF2–IRF4 axis maintains myeloma cell survival
Authors: Ohguchi Hiroto , Hideshima Teru , Bhasin Manoj K. , Gorgun Gullu T. , Santo Loredana , Cea Michele , Samur Mehmet K. , Mimura Naoya , Suzuki Rikio , Tai Yu-Tzu , Carrasco Ruben D. , Raje Noopur , Richardson Paul G. , Munshi Nikhil C. , Harigae Hideo , Sanda Takaomi , Sakai Juro , Anderson Kenneth C. 
Published: January 2016

AbstractKDM3A is implicated in tumorigenesis; however, its biological role in multiple myeloma (MM) has not been elucidated. Here we identify KDM3A–KLF2–IRF4 axis dependence in MM. Knockdown of KDM3A is toxic to MM cells in vitro and in vivo. KDM3A maintains expression of KLF2 and IRF4 through H3K9 demethylation, and knockdown of KLF2 triggers apoptosis. Moreover, KLF2 directly activates IRF4 and IRF4 reciprocally upregulates KLF2, forming a positive autoregulatory circuit. The interaction of MM cells with bone marrow milieu mediates survival of MM cells. Importantly, silencing of KDM3A, KLF2 or IRF4 both decreases MM cell adhesion to bone marrow stromal cells and reduces MM cell homing to the bone marrow, in association with decreased ITGB7 expression in MAF-translocated MM cell lines. Our results indicate that the KDM3A–KLF2–IRF4 pathway plays an essential role in MM cell survival and homing to the bone marrow, and therefore represents a therapeutic target.
DCAF1 controls T-cell function via p53-dependent and -independent mechanisms
Authors: Guo Zengli , Kong Qing , Liu Cui , Zhang Song , Zou Liyun , Yan Feng , Whitmire Jason K. , Xiong Yue , Chen Xian , Wan Yisong Y. 
Published: January 2016

AbstractOn activation, naive T cells grow in size and enter cell cycle to mount immune response. How the fundamental processes of T-cell growth and cell cycle entry are regulated is poorly understood. Here we report that DCAF1 (Ddb1–cullin4-associated-factor 1) is essential for these processes. The deletion of DCAF1 in T cells impairs their peripheral homeostasis. DCAF1 is upregulated on T-cell receptor activation and critical for activation-induced T-cell growth, cell cycle entry and proliferation. In addition, DCAF1 is required for T-cell expansion and function during anti-viral and autoimmune responses in vivo. DCAF1 deletion leads to a drastic stabilization of p53 protein, which can be attributed to a requirement of DCAF1 for MDM2-mediated p53 poly-ubiquitination. Importantly, p53 deletion rescues the cell cycle entry defect but not the growth defect of DCAF1-deficient cells. Therefore, DCAF1 is vital for T-cell function through p53-dependent and -independent mechanisms.
Comprehensive systems biology analysis of a 7-month cigarette smoke inhalation study in C57BL/6 mice
Authors: Ansari Sam , Baumer Karine , Boué Stéphanie , Dijon Sophie , Dulize Remi , Ekroos Kim , Elamin Ashraf , Foong Clement , Guedj Emmanuel , Hoeng Julia , Ivanov Nikolai V. , Krishnan Subash , Leroy Patrice , Martin Florian , Merg Celine , Peck Michael J. , Peitsch Manuel C. , Phillips Blaine , Schlage Walter K. , Schneider Thomas , Talikka Marja , Titz Bjoern , Vanscheeuwijck Patrick , Veljkovic Emilija , Vihervaara Terhi , Vuillaume Gregory , Woon Ching Qing 
Published: January 2016

AbstractSmoking of combustible cigarettes has a major impact on human health. Using a systems toxicology approach in a model of chronic obstructive pulmonary disease (C57BL/6 mice), we assessed the health consequences in mice of an aerosol derived from a prototype modified risk tobacco product (pMRTP) as compared to conventional cigarettes. We investigated physiological and histological endpoints in parallel with transcriptomics, lipidomics, and proteomics profiles in mice exposed to a reference cigarette (3R4F) smoke or a pMRTP aerosol for up to 7 months. We also included a cessation group and a switching-to-pMRTP group (after 2 months of 3R4F exposure) in addition to the control (fresh air-exposed) group, to understand the potential risk reduction of switching to pMRTP compared with continuous 3R4F exposure and cessation. The present manuscript describes the study design, setup, and implementation, as well as the generation, processing, and quality control analysis of the toxicology and ‘omics’ datasets that are accessible in public repositories for further analyses.
Predicting and verifying outcome of Tripterygium wilfordii Hook F. based therapy in rheumatoid arthritis: from open to double-blinded randomized trial
Authors: Jiang Miao , Zha Qinglin , Zhang Chi , Lu Cheng , Yan Xiaoping , Zhu Wanhua , Liu Wei , Tu Shenghao , Hou Liping , Wang Chengwu , Zhang Wandong , Liang Qinghua , Fan Bing , Yu Jiangping , Zhang Weidong , Liu Xinru , Yang Jing , He Xiaojuan , Li Li , Niu Xuyan , Liu Yan , Guo Hongtao , He Bing , Zhang Ge , Bian Zhaoxiang , Lu Aiping 
Published: January 2016

AbstractTripterygium wilfordii Hook F. (TwHF) based therapy has been proved as effective in treating rheumatoid arthritis (RA), yet the predictors to its response remains unclear. A two-stage trial was designed to identify and verify the baseline symptomatic predictors of this therapy. 167 patients with active RA were enrolled with a 24-week TwHF based therapy treatment and the symptomatic predictors were identified in an open trial; then in a randomized clinical trial (RCT) for verification, 218 RA patients were enrolled and classified into predictor positive (P+) and predictor negative (P−) group and were randomly assigned to accept the TwHF based therapy and Methotrexate and Sulfasalazine combination therapy (M&S) for 24 weeks, respectively. Five predictors were identified (diuresis, excessive sweating, night sweats for positive; and yellow tongue-coating, thermalgia in the joints for negative). In the RCT, The ACR 20 responses were 82.61% in TwHF/P+ group, significantly higher than that in TwHF/P− group (P = 0.0001) and in M&S/P+ group (P < 0.05), but not higher than in M&S/P− group. Similar results were yielded in ACR 50 yet not in ACR 70 response. No significant differences were detected in safety profiles among groups. The identified predictors enable the TwHF based therapy more efficiently in treating RA subpopulations.
Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata
Authors: Zhang Dan-Dan , Wang Hong-Lei , Schultze Anna , Froß Heidrun , Francke Wittko , Krieger Jürgen , Löfstedt Christer 
Published: January 2016

AbstractHow signal diversity evolves under stabilizing selection in a pheromone-based mate recognition system is a conundrum. Female moths produce two major types of sex pheromones, i.e., long-chain acetates, alcohols and aldehydes (Type I) and polyenic hydrocarbons and epoxides (Type II), along different biosynthetic pathways. Little is known on how male pheromone receptor (PR) genes evolved to perceive the different pheromones. We report the identification of the first PR tuned to Type II pheromones, namely ObruOR1 from the winter moth,Operophtera brumata(Geometridae). ObruOR1 clusters together with previously ligand-unknown orthologues in the PR subfamily for the ancestral Type I pheromones, suggesting thatO. brumatadid not evolve a new type of PR to match the novel Type II signal but recruited receptors within an existing PR subfamily. AsegOR3, the ObruOR1 orthologue previously cloned from the noctuidAgrotis segetumthat has Type I acetate pheromone components, responded significantly to another Type II hydrocarbon, suggesting that a common ancestor with Type I pheromones had receptors for both types of pheromones, a preadaptation for detection of Type II sex pheromone.