bluuebunny/crossref_metadata_2025_split huggingface dataset:
The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana
Authors: Wang Linjuan , Li Houhua , Zhao Chunzhao , Li Shengfei , Kong Lingyao , Wu Wenwu , Kong Weisheng , Liu Yan , Wei Yuanyuan , Zhu Jian‐Kang , Zhang Hairong 
Published: August 2016

AbstractIn yeast, the interaction of General Control Non‐derepressible 1 (GCN1) with GCN2 enables GCN2 to phosphorylate eIF2α (the alpha subunit of eukaryotic translation initiation factor 2) under a variety of stresses. Here, we cloned AtGCN1, an Arabidopsis homologue of GCN1. We show that AtGCN1 directly interacts with GCN2 and is essential for the phosphorylation of eIF2α under salicylic acid (SA), ultraviolet (UV), cold stress and amino acid deprivation conditions. Two mutant alleles, atgcn1‐1 and atgcn1‐2, which are defective in the phosphorylation of eIF2α, showed increased sensitivity to cold stress, compared with the wild type. Ribosome‐bound RNA profiles showed that the translational state of mRNA was higher in atgcn1‐1 than in the wild type. Our result also showed that cold treatment reduced the tendency of the tor mutant seedlings to produce purple hypocotyls. In addition, the kinase activity of TOR was transiently inhibited when plants were exposed to cold stress, suggesting that the inhibition of TOR is another pathway important for plants to respond to cold stress. In conclusion, our results indicate that the AtGCN1‐mediated phosphorylation of eIF2α, which is required for inhibiting the initiation of protein translation, is essential for cold tolerance in Arabidopsis.
Alice in Wonderland syndrome associated with mycoplasma infection
Authors: Omata Taku , Fujii Katsunori , Kuroki Haruo , Shimojo Naoki 
Published: August 2016

AbstractAlice in Wonderland syndrome (AIWS) is a rare condition in which patients report distorted size perception of objects and their own bodies. Although specific causes and pathology have not been elucidated, an association between AIWS and infection has been suggested. To our knowledge, mycoplasma‐induced AIWS has not been examined. A girl aged 7 years 11 months presented with fever (temperature, 40°C) and cough. Although the fever disappeared after approximately 10 days, she complained that her mother's face suddenly appeared smaller to her. Subsequently, she complained that objects intermittently appeared smaller than normal. Particle agglutination test indicated elevated serum antibodies against Mycoplasma pneumoniae. The patient was therefore diagnosed the patient with AIWS secondary to mycoplasma infection. Although mycoplasma infection is known to cause various central nervous system symptoms, this is the first report involving AIWS, suggesting that mycoplasma could affect visual function in children.
Effects of relationship education on emerging adults' relationship beliefs and behaviors
Authors: HOLT LAURA J. , MATTANAH JONATHAN F. , SCHMIDT CHRISTA K. , DAKS JENNIFER S. , BROPHY ERIN N. , MINNAAR PAULINE Y. , RORER KATHERINE 
Published: August 2016

AbstractIn this multisite study, we used a randomized controlled trial to examine how a relationship education intervention affected emerging adults' maladaptive relationship beliefs, mutuality, relationship decision making, relationship quality, and psychological distress. In addition, we explored whether one intervention modality (i.e., facilitated group discussion) was more/less effective than another (i.e., self‐facilitated online) and whether there were differential effects for participants in a relationship. We found that the facilitated group evidenced a decline in maladaptive relationship beliefs and an improvement in deliberate decision making. Moreover, facilitated group participants in a relationship reported higher levels of mutuality. Contrary to our hypothesis, there were no changes in relationship quality or psychological distress. Implications for the content and delivery of relationship education for emerging adults are discussed.
Effects of acute rejection vs new‐onset diabetes after transplant on transplant outcomes in pediatric kidney recipients: analysis of the Organ Procurement and Transplant Network/United Network for Organ Sharing (OPTN/UNOS) database
Authors: Mehrnia Alireza , Le Thuy X. , Tamer Tamer R. , Bunnapradist Suphamai 
Published: August 2016

AbstractImproving long‐term transplant and patient survival is still an ongoing challenge in kidney transplant medicine. Our objective was to identify the subsequent risks of new‐onset diabetes after transplant (NODAT) and acute rejection (AR) in the first year post‐transplant in predicting mortality and transplant failure. A total of 4687 patients without preexisting diabetes (age 2–20 years, 2004–2010) surviving with a functioning transplant for longer than 1 year with at least one follow‐up report were identified from the OPTN/UNOS database as of September 2014. Study population was stratified into four mutually exclusive groups: Group 1, patients with a history of AR; Group 2, NODAT+; Group 3, NODAT+ AR+; and Group 4, the reference group (neither). Multivariate regression was used to analyze the relative risks for the outcomes of transplant failure and mortality. The median follow‐up time was 1827 days after 1 year post‐transplant. AR was associated with an increased risk of adjusted graft and death‐censored graft failure (HR 2.87, CI 2.48–3.33, P < .001 and HR 2.11, CI 1.81–2.47, P < .001), respectively. NODAT and AR were identified in 3.5% and 14.5% of all study patients, respectively. AR in the first year post‐transplant was a major risk factor for overall and death‐censored graft failure, but not mortality. However, NODAT was not a risk factor on graft survival or mortality.
A complication probability planning study to predict the safety of a new protocol for intracranial tumour radiotherapy in dogs
Authors: Rohrer Bley C. , Meier V. , Schwarz P. , Roos M. , Besserer J. 
Published: August 2016

AbstractTechnical advances make it possible to deliver radiation therapy for canine intracranial tumours in fewer fractions, under the assumption of equivalent tumour control. With the aim of estimating the late toxicity risk profile for various tumour sizes and locations, the present paper evaluates the normal tissue complication probability (NTCP) values for the intracranial organs at risk. By making isoeffect calculations, a new 10‐fraction radiation protocol was developed with the same tumour control probability (TCP) as a currently used 20‐fraction standard protocol, and complication risk profiles for brain, brainstem and optic chiasm were modelled using a representative population of 64 dogs with brain tumours. For >59% of cases, the new 10‐fraction protocol yielded an acceptable, low risk estimate of late toxicity (<10%). Our calculations suggest that it may be safe to treat small to intermediate‐sized tumours that are neither located near the optic chiasm nor at the brainstem with 10 daily fractions of 4.35 Gy.
A Framework for Improving the Quality of Research in the Biological Sciences
Authors: Casadevall Arturo , Ellis Lee M. , Davies Erika W. , McFall-Ngai Margaret , Fang Ferric C. 
Published: August 2016

ABSTRACT
The American Academy of Microbiology convened a colloquium to discuss problems in the biological sciences, with emphasis on identifying mechanisms to improve the quality of research. Participants from various disciplines made six recommendations: (i) design rigorous and comprehensive evaluation criteria to recognize and reward high-quality scientific research; (ii) require universal training in good scientific practices, appropriate statistical usage, and responsible research practices for scientists at all levels, with training content regularly updated and presented by qualified scientists; (iii) establish open data at the timing of publication as the standard operating procedure throughout the scientific enterprise; (iv) encourage scientific journals to publish negative data that meet methodologic standards of quality; (v) agree upon common criteria among scientific journals for retraction of published papers, to provide consistency and transparency; and (vi) strengthen research integrity oversight and training. These recommendations constitute an actionable framework that, in combination, could improve the quality of biological research.
How do people of South Asian origin understand and experience depression? A protocol for a systematic review of qualitative literature
Authors: Mooney Roisin , Trivedi Daksha , Sharma Shivani 
Published: August 2016

IntroductionIndividuals from Black and Asian Minority Ethnic (BAME) groups are less likely to receive a diagnosis and to engage with treatment for depression. This review aims to draw on international literature to summarise what is known about how people specifically of South Asian origin, migrants and non-migrants, understand and experience depressive symptoms. The resulting evidence base will further inform practices aimed at encouraging help-seeking behaviour and treatment uptake.Methods and analysisA systematic review and thematic synthesis of qualitative literature conducted according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. Using predefined inclusion and exclusion criteria, electronic searches will be conducted across 16 databases. Study quality will be assessed using the Critical Appraisal Skills Programme (CASP). Data will be extracted independently by 2 reviewers.Ethics and disseminationEthical approval is not required. A comprehensive evidence base of how people from South Asian backgrounds conceptualise and experience depression will better inform the design and delivery of mental health initiatives and advance directions for future research. Findings will be published in a peer-reviewed journal, and disseminated through existing networks for professionals, researchers, patients and the public.Trial registration numberCRD42015026120.
Transverse momentum distribution of primary charged particles in the p–Pb interactions using HIJING 1.0
Authors: Tabassam U. , Ali Y. , Suleymanov M. , Bhatti A. S. , Butt J. B. , Amjad M. S. 
Published: August 2016

The shape of the transverse momentum [Formula: see text] distribution of primary charged particles in minimum bias (nonsingle-diffractive) p–Pb collisions at [Formula: see text] is studied in the pseudorapidity regions: [Formula: see text], [Formula: see text] and [Formula: see text] and in the transverse momentum range [Formula: see text] using simulated data produced with the HIJING 1.0 code. These are compared with the ALICE data measured by the ALICE detector at the LHC. In the model, the central and forward [Formula: see text]-regions differ more than in the ALICE data and due to this fact HIJING 1.0 cannot describe well the high [Formula: see text] region in the [Formula: see text] distributions. The comparison of results from simulation implies that the HIJING 1.0 considered narrower pseudorapidity distribution for the charged particles than it is in the ALICE data. It cannot take into account satisfactorily leading effect due to the asymmetric p–Pb fragmentation.
On planar caustics
Authors: Goryunov Victor , Gallagher Katy 
Published: August 2016

We study local invariants of planar caustics, that is, invariants of Lagrangian maps from surfaces to [Formula: see text] whose increments in generic homotopies are determined entirely by diffeomorphism types of local bifurcations of the caustics. Such invariants are dual to trivial codimension 1 cycles supported on the discriminant in the space [Formula: see text] of the Lagrangian maps. We obtain a description of the spaces of the discriminantal cycles (possibly non-trivial) for the Lagrangian maps of an arbitrary surface, both for the integer and mod 2 coefficients. It is shown that all integer local invariants of caustics of Lagrangian maps without corank 2 points are essentially exhausted by the numbers of various singular points of the caustics and the Ohmoto–Aicardi linking invariant of ordinary maps. As an application, we use the discriminantal cycles to establish non-contractibility of certain loops in [Formula: see text].
Dynamical Gelfand–Zetlin algebra and equivariant cohomology of grassmannians
Authors: Rimanyi R. , Varchenko A. 
Published: August 2016

We consider the rational dynamical quantum group [Formula: see text] and introduce an [Formula: see text]-module structure on [Formula: see text], where [Formula: see text] is the equivariant cohomology algebra [Formula: see text] of the cotangent bundle of the Grassmannian [Formula: see text] with coefficients extended by a suitable ring of rational functions in an additional variable [Formula: see text]. We consider the dynamical Gelfand–Zetlin algebra which is a commutative algebra of difference operators in [Formula: see text]. We show that the action of the Gelfand–Zetlin algebra on [Formula: see text] is the natural action of the algebra [Formula: see text] on [Formula: see text], where [Formula: see text] is the shift operator. The [Formula: see text]-module structure on [Formula: see text] is introduced with the help of dynamical stable envelope maps which are dynamical analogs of the stable envelope maps introduced by Maulik and Okounkov [Quantum Groups and quantum cohomology, preprint (2012) 1–276, arXiv:1211.1287]. The dynamical stable envelope maps are defined in terms of the rational dynamical weight functions introduced in [G. Felder, V. Tarasov and A. Varchenko, Solutions of the elliptic QKZB equations and Bethe ansatz I, in Topics in Singularity Theory V. I. Arnold’s 60th Anniversary Collection, Advances in the Mathematical Sciences, AMS Translations, Series 2, Vol. 180 (AMS, 1997), pp. 45–76.] to construct q-hypergeometric solutions of rational qKZB equations. The cohomology classes in [Formula: see text] induced by the weight functions are dynamical variants of Chern–Schwartz–MacPherson classes of Schubert cells.
Wnt Signaling Promotes Breast Cancer by Blocking ITCH-Mediated Degradation of YAP/TAZ Transcriptional Coactivator WBP2
Authors: Lim Shen Kiat , Lu Ssu Yi , Kang Shin-Ae , Tan Hock Jin , Li Zilin , Adrian Wee Zhen Ning , Guan Jye Swei , Reddy Chichili Vishnu Priyanka , Sivaraman J. , Putti Thomas , Thike Aye Aye , Tan Puay Hoon , Sudol Marius , Virshup David M. , Chan Siew Wee , Hong Wanjin , Lim Yoon Pin 
Published: August 2016

Abstract
Cross-talk between the Hippo and Wnt pathways has been implicated recently in breast cancer development, but key intersections have yet to be fully defined. Here we report that WBP2, a transcription coactivator that binds the Hippo pathway transcription factor YAP/TAZ, contributes to Wnt signaling and breast cancer pathogenesis. Clinically, overexpression of WBP2 in breast cancer specimens correlated with malignant progression and poor patient survival. In breast cancer cells, nuclear entry and interaction of WBP2 with β-catenin was stimulated by Wnt3A, thereby activating TCF-mediated transcription and driving malignant invasive character. Mechanistic investigations showed WBP2 levels were controlled by the E3 ligase ITCH, which bound and target WBP2 for ubiquitin-dependent proteasomal degradation. Accordingly, ITCH silencing could elevate WBP2 levels. Wnt signaling upregulated WBP2 by disrupting ITCH–WBP2 interactions via EGFR-mediated tyrosine phosphorylation of WBP2 and TAZ/YAP competitive binding. Conversely, ITCH-mediated downregulation of WBP2 inhibited TCF/β-catenin transcription, in vitro transformation, and in vivo tumorigenesis. We identified somatic mutations in ITCH, which impaired its ability to degrade WBP2 and to block its function in cancer, even while retaining binding capacity to WBP2. Thus, the Wnt pathway appeared to engage WBP2 primarily by affecting its protein stability. Our findings show how WBP2/ITCH signaling functions to link the intricate Wnt and Hippo signaling networks in breast cancer. Cancer Res; 76(21); 6278–89. ©2016 AACR.
Extracellular ATP a New Player in Cancer Metabolism: NSCLC Cells Internalize ATP In Vitro and In Vivo Using Multiple Endocytic Mechanisms
Authors: Qian Yanrong , Wang Xuan , Li Yunsheng , Cao Yanyang , Chen Xiaozhuo 
Published: August 2016

Abstract
Intratumoral extracellular ATP concentrations are 1000 times higher than those in normal tissues of the same cell origin. However, whether or not cancer cells use the abundant extracellular ATP was unknown until we recently reported that cancer cells internalize ATP. The internalized ATP was found to substantially increase intracellular ATP concentration and promote cell proliferation and drug resistance in cancer cells. Here, using a nonhydrolyzable fluorescent ATP (NHF-ATP), radioactive and regular ATP, coupled with high and low molecular weight dextrans as endocytosis tracers and fluorescence microscopy and ATP assays, cultured human NSCLC A549 and H1299 cells as well as A549 tumor xenografts were found to internalize extracellular ATP at concentrations within the reported intratumoral extracellular ATP concentration range. In addition to macropinocytosis, both clathrin- and caveolae-mediated endocytosis significantly contribute to the ATP internalization, which led to an approximately 30% (within 45 minutes) or more than 50% (within 4 hours) increase in intracellular ATP levels after ATP incubation. This increase could not be accounted for by either purinergic receptor signaling or increased intracellular ATP synthesis rates in the ATP-treated cancer cells. These new findings significantly deepen our understanding of the Warburg effect by shedding light on how cancer cells in tumors, which are heterogeneous for oxygen and nutrition supplies, take up extracellular ATP and use the internalized ATP to perform multiple previously unrecognized functions of biological importance. They strongly suggest the existence of ATP sharing among cancer and stromal cells in tumors and simultaneously identify multiple new anticancer targets.
Implications: Extracellular ATP is taken up by human lung cancer cells and tumors via macropinocytosis and other endocytic processes to supplement their extra energy needs for cancer growth, survival, and drug resistance, thus providing novel targets for future cancer therapy. Mol Cancer Res; 14(11); 1087–96. ©2016 AACR.
The EBC TWO Study (European Bifurcation Coronary TWO)
Authors: Hildick-Smith David , Behan Miles W. , Lassen Jens F. , Chieffo Alaide , Lefèvre Thierry , Stankovic Goran , Burzotta Francesco , Pan Manuel , Ferenc Miroslaw , Bennett Lorraine , Hovasse Thomas , Spence Mark S. , Oldroyd Keith , Brunel Philippe , Carrie Didier , Baumbach Andreas , Maeng Michael , Skipper Nicola , Louvard Yves 
Published: August 2016

Background—
For the treatment of coronary bifurcation lesions, a provisional strategy is superior to systematic 2-stent techniques for the most bifurcation lesions. However, complex anatomies with large side branches (SBs) with significant ostial disease length are considered by expert consensus to warrant a 2-stent technique upfront. This consensus view has not been scientifically assessed.

Methods and Results—

Symptomatic patients with large caliber true bifurcation lesions (SB diameter ≥2.5 mm) and significant ostial disease length (≥5 mm) were randomized to either a provisional T-stent strategy or a dual stent culotte technique. Two hundred patients aged 64±10 years, 82% male, were randomized in 20 European centers. The clinical presentations were stable coronary disease (69%) and acute coronary syndromes (31%). SB stent diameter (2.67±0.27 mm) and length (20.30±5.89 mm) confirmed the extent of SB disease. Procedural success (provisional 97%, culotte 94%) and kissing balloon inflation (provisional 95%, culotte 98%) were high. Sixteen percent of patients in the provisional group underwent T-stenting. The primary end point (a composite of death, myocardial infarction, and target vessel revascularization at 12 months) occurred in 7.7% of the provisional T-stent group versus 10.3% of the culotte group (hazard ratio, 1.02; 95% confidence interval, 0.78–1.34;
P
=0.53). Procedure time, x-ray dose, and cost all favored the simpler procedure.

Conclusions—
When treating complex coronary bifurcation lesions with large stenosed SBs, there is no difference between a provisional T-stent strategy and a systematic 2-stent culotte strategy in a composite end point of death, myocardial infarction, and target vessel revascularization at 12 months.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT 01560455.
Prevention of Stroke with Ticagrelor in Patients with Prior Myocardial Infarction
Authors: Bonaca Marc P. , Goto Shinya , Bhatt Deepak L. , Steg P. Gabriel , Storey Robert F. , Cohen Marc , Goodrich Erica , Mauri Laura , Ophuis Ton Oude , Ruda Mikhail , Špinar Jindřich , Seung Ki-Bae , Hu Dayi , Dalby Anthony J. , Jensen Eva , Held Peter , Morrow David A. , Braunwald Eugene , Sabatine Marc S. 
Published: August 2016

Background:
In the PEGASUS-TIMI 54 trial (Prevention of Cardiovascular Events in Patients With Prior Heart Attack Using Ticagrelor Compared to Placebo on a Background of Aspirin-Thrombolysis in Myocardial Infarction 54), ticagrelor reduced the risk of major adverse cardiovascular events when added to low-dose aspirin in stable patients with prior myocardial infarction, resulting in the approval of ticagrelor 60 mg twice daily for long-term secondary prevention. We investigated the incidence of stroke, outcomes after stroke, and the efficacy of ticagrelor focusing on the approved 60 mg twice daily dose for reducing stroke in this population.

Methods:
Patients were followed for a median of 33 months. Stroke events were adjudicated by a central committee. Data from similar trials were combined using meta-analysis.

Results:

Of 14 112 patients randomly assigned to placebo or ticagrelor 60 mg, 213 experienced a stroke; 85% of these strokes were ischemic. A total of 18% of strokes were fatal and another 15% led to either moderate or severe disability at 30 days. Ticagrelor significantly reduced the risk of stroke (hazard ratio, 0.75; 95% confidence interval, 0.57–0.98;
P
=0.034), driven by a reduction in ischemic stroke (hazard ratio, 0.76; 95% confidence interval, 0.56–1.02). Hemorrhagic stroke occurred in 9 patients on placebo and 8 patients on ticagrelor. A meta-analysis across 4 placebo-controlled trials of more intensive antiplatelet therapy in 44 816 patients with coronary disease confirmed a marked reduction in ischemic stroke (hazard ratio, 0.66; 95% confidence interval, 0.54–0.81;
P
=0.0001).

Conclusions:
High-risk patients with prior myocardial infarction are at risk for stroke, approximately one-third of which are fatal or lead to moderate-to-severe disability. The addition of ticagrelor 60 mg twice daily significantly reduced this risk without an excess of hemorrhagic stroke but with more major bleeding. In high-risk patients with coronary disease, more intensive antiplatelet therapy should be considered not only to reduce the risk of coronary events, but also of stroke.

Clinical Trial Registration:

URL:
http://www.clinicaltrials.gov
. Unique Identifier: NCT01225562.