bluuebunny/crossref_metadata_2025_split huggingface dataset:
A glimpse into evolution and dissemination of multidrug-resistant Acinetobacter baumannii isolates in East Asia: a comparative genomics study
Authors: Feng Ye , Ruan Zhi , Shu Jianfeng , Chen Chyi-Liang , Chiu Cheng-Hsun 
Published: April 2016

AbstractClonal dissemination is characteristic of the important nosocomial pathogen Acinetobacter baumannii, as revealed by previous multi-locus sequence typing (MLST) studies. However, the disseminated phyletic unit is actually MLST sequence type instead of real bacterial clone. Here we sequenced the genomes of 13 multidrug-resistant (MDR) A. baumannii strains from Taiwan and compared them with that of A. baumannii from other East Asian countries. Core-genome phylogenetic tree divided the analyzed strains into three major clades. Among them, one ST455 clade was a hybrid between the ST208 clade and the other ST455 clade. Several strains showed nearly identical genome sequence, but their isolation sources differed by over 2,500 km and 10 years apart, suggesting a wide dissemination of the phyletic units, which were much smaller than the sequence type. Frequent structural variation was detected even between the closely related strains in antimicrobial resistance elements such as AbaRI, class I integron, indicating strong selection pressure brought by antimicrobial use. In conclusion, wide clonal dissemination and frequent genomic variation simultaneously characterize the clinical MDR A. baumannii in East Asia.
Long-Lasting Cross-Protection Against Influenza A by Neuraminidase and M2e-based immunization strategies
Authors: Schotsaert Michael , Ysenbaert Tine , Smet Anouk , Schepens Bert , Vanderschaeghe Dieter , Stegalkina Svetlana , Vogel Thorsten U. , Callewaert Nico , Fiers Walter , Saelens Xavier 
Published: April 2016

AbstractThere is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and H3N2 viruses of mice that had been immunized with hemagglutinin (HA), neuraminidase (NA) and the extracellular domain of matrix protein 2 (M2e) fused to a virus-like particle (VLP). Mice were challenged a first time with a sublethal dose of H1N1 2009 pandemic virus and, four weeks later, challenged again with an H3N2 virus. Mice that had been vaccinated with HA, NA, NA + M2e-VLP and HA + NA + M2e-VLP were protected against homologous H1N1 virus challenge. Challenged NA and NA + M2e-VLP vaccinated mice mounted CD8+ T cell responses that correlated with protection against secondary H3N2 challenge. HA-vaccinated mice were fully protected against challenge with homologous H1N1 2009 virus, failed to mount cross-reactive CD8+ T cells and succumbed to the second challenge with heterologous H3N2 virus. In summary, NA- and M2e-based immunity can protect against challenge with (homologous) virus without compromising the induction of robust cross-reactive CD8+ T cell responses upon exposure to virus.
Further Study on the Relation of Polyoxin Structure to Chitin Synthetase Inhibition
Authors: Hori Masahiro , Kakiki Kazuo , Misato Tomomasa 
Published: April 2016

Abstract
As a part of studies on the mechanism of action of antibiotics polyoxins, effects of various N-aminoacyl derivatives of polyoxin C and other polyoxins on chitin-UDP acetylglucosaminyl-transferase (EC 2.4.1.16, chitin synthetase) prepared from phytopathogenic fungus Piricularia oryzae were investigated. It was found that they inhibited the enzyme in competition with the substrate UDP-N-acetylglucosamine, Inhibitor constants, Ki, for these polyoxins were determined and the values of binding affinity, −ΔGbind of the inhibitors to the enzyme were calculated from the Ki values. In addition, by using these −ΔGbind values the values of partial binding affinity, −Δg for the several atoms and atomic groups or the several moieties contained in the polyoxin J molecule were estimated. From the results obtained, it was concluded that the carbamoylpolyoxamic acid moiety of polyoxins helps to stabilize the polyoxin-enzyme complex through the contributions of its oxygen atom at C−1″, amino group at C−2″, hydroxyl groups at C−3″ and C−4″, aliphatic carbon chain and terminal carbamoyloxy group.
The results obtained by the kinetic investigation using various nucleotides and nucleotide sugars suggested that there was a specific binding site on the enzyme corresponding to the uridine moiety of UDP-N-acetylglucosamine, and that the pyrimidine nucleoside moiety of polyoxins was also bound to this site.
Studies of the Effect of Tenuazonic Acid on Plant Cells and Seedlings
Authors: Umetsu Noriharu , Muramatsu Takeharu , Honda Hiroshi , Tamari Kinjiro 
Published: April 2016

Abstract
The biological and biochemical studies of the effect of tenuazonic acid on plant cells and seedlings were carried out. Tenuazonic acid exhibited a conspicuous stunting effect on the seedling-growth of rice plant, mung bean, radish and turnip, and on the growth of suspension cultured cells of soybean and rice plants. Tenuazonic acid exhibited no effect on the O2-uptake and the activity of SH-enzyme of the plant, but inhibited the incorporation of 14C-Ieucine into the protein fraction and that of 14C-adenine into nucleic acid fraction of suspension cultured soybean cells as well as these uptake into the cells. And then it has been proved that these incorporation-inhibitions were not merely due to the inhibition of 14C-leucine and 14C-adenine uptake into the cells but based on the intrinsic inhibition of protein and nucleic acid syntheses, respectively.
Conversion of ( + )-Carvone by Pseudomonas ovalis, Strain 6-1 (1)
Authors: Noma Yoshiaki , Nonomura Seiichi , Ueda Hiroo , Tatsumi Chuji 
Published: April 2016

Abstract
The conversion of (+)-carvone by Pseudomonas ovalis, strain 6-1, was investigated. (+)-Carvone was found to be reduced to (−)-isodihydrocarvone, (−)-isodihydrocarveol, (−)-neoisodihydrocarveol, (−)-dihydrocarvone, (−)-neodihydrocarveol, and (+)-dihydrocarveol, of which the former three were the major products.
From these results, it was postulated that Pseudomonas ovalis, strain 6-1, has different pathways for (+)-carvone and (−)-carvone, respectively; (+)-carvone is converted via (−)- isodihydrocarvone to (−)-isodihydrocarveol and (−)-neoisodihydrocarveol, whereas (−)- carvone is converted via (+)-dihydrocarvone to (−)-dihydrocarveol.
Stereochemical structures of four isomers of dihydrocarveols were also discussed on the basis of PMR results.
Mode of Action of Clostridium Phage HM 7-Induced Lytic Enzyme on Clostridium saccharoperbutylacetonicum Cell Wall Peptidoglycan
Authors: Ogata Seiya , Tahara Yasutaka , Hongo Motoyoshi 
Published: April 2016

Abstract
The action of Clostridium phage HM 7-induced lytic enzyme on the cell wall peptidoglycan of Clostridium saccharoperbutylacetonicum was investigated. The cell wall peptidoglycan of this strain contained glutamic acid, alanine, diaminopimelic acid, glucosamine and muramic acid in the molar ratios of 1.00: 2.08: 0.97; 0.92: 0.68. It was strongly digested when incubated with the lytic enzyme. This digestion was accompanied by the release of NH2-terminal l-alanine without a concomitant release of COOH-terminal amino acids and reducing groups. Chromatography of the lytic enzyme digest resulted in only two fractions, each of which was chromatographically homogeneous. One was a polysaccharide consisting of glucosamine and muramic acid in molar ratios 1.00: 0.78, and other was a peptide composed of glutamic acid, alanine and diaminopimelic acid in molar ratios of 1.00: 2.09: 1.05. These results indicate that phage HM 7-induced lytic enzyme is N-acetylmuramyl-l-alanine amidase, which cleaves the linkage between N-acetylmuramic acid and l-alanine.
A possible structure for the cell wall peptidoglycan was also proposed.
Antifeeding Active Substances for Insect in Caryopteris divaricata Maxim.
Authors: Hosozawa Shigeki , Kato Natsuki , Munakata Katsura 
Published: April 2016

Abstract
Six antifeeding active diterpenes having a clerodane skeleton, clerodin (I), caryoptin (II), dihydrocĩerodin-I (V), dihydrocaryoptin (VI), clerodin hemiacetal (VII), and caryoptin hemi- acetal (VIII), were isolated from Caryopteris divaricata Maxim. Antifeeding activities of six compounds and these derivatives against the 3rd instar larvae of Spodoptera litura F. were tested by the leaf disk method. In addition, the terms of “relative antifeedant” and “absolute antifeedant” were proposed for the antifeeding substances, and the latter term was used to the above six diterpenes.
Five new fast radio bursts from the HTRU high-latitude survey at Parkes: first evidence for two-component bursts
Authors: Champion D. J. , Petroff E. , Kramer M. , Keith M. J. , Bailes M. , Barr E. D. , Bates S. D. , Bhat N. D. R. , Burgay M. , Burke-Spolaor S. , Flynn C. M. L. , Jameson A. , Johnston S. , Ng C. , Levin L. , Possenti A. , Stappers B. W. , van Straten W. , Thornton D. , Tiburzi C. , Lyne A. G. 
Published: April 2016

Abstract
The detection of five new fast radio bursts (FRBs) found in the 1.4-GHz High Time Resolution Universe high-latitude survey at Parkes, is presented. The rate implied is 7$^{+5}_{-3}\times 10^3$ (95 per cent) FRBs sky−1 d−1 above a fluence of 0.13 Jy ms for an FRB of 0.128 ms duration to 1.5 Jy ms for 16 ms duration. One of these FRBs has a two-component profile, in which each component is similar to the known population of single component FRBs and the two components are separated by 2.4 ± 0.4 ms. All the FRB components appear to be unresolved following deconvolution with a scattering tail and accounting for intrachannel smearing. The two-component burst, FRB 121002, also has the highest dispersion measure (1629 pc cm−3) of any FRB to-date. Many of the proposed models to explain FRBs use a single high-energy event involving compact objects (such as neutron-star mergers) and therefore cannot easily explain a two-component FRB. Models that are based on extreme versions of flaring, pulsing, or orbital events, however, could produce multiple component profiles. The compatibility of these models and the FRB rate implied by these detections is discussed.
Microevolution of bank voles (Myodes glareolus) at neutral and immune-related genes during multiannual dynamic cycles: Consequences for Puumala hantavirus epidemiology
Authors: Adelaïde Dubois , Galan Maxime , Cosson Jean-François , Gauffre Bertrand , Henttonen Heikki , Niemimaa Jukka , Razzauti Maria , Voutilainen Liina , Vitalis Renaud , Guivier Emmanuel , Charbonnel Nathalie 
Published: April 2016

ABSTRACTUnderstanding how host dynamics, including variations of population size and dispersal, may affect the epidemiology of infectious diseases through ecological and evolutionary processes is an active research area. Here we focus on a bank vole (Myodes glareolus) metapopulation surveyed in Finland between 2005 and 2009. Bank vole is the reservoir of Puumala hantavirus (PUUV), the agent of nephropathia epidemica (NE, a mild form of hemorrhagic fever with renal symptom) in humans.M glareoluspopulations experience multiannual density fluctuations that may influence the level of genetic diversity maintained in bank voles, PUUV prevalence and NE occurrence. We examine bank vole metapopulation genetics at presumably neutral markers and immune-related genes involved in susceptibility to PUUV (Tnf-promoter,Mhc-Drb, Tlr4,Tlr7andMx2gene) to investigate the links between population dynamics, microevolutionary processes and PUUV epidemiology. We show that genetic drift slightly and transiently affects neutral and adaptive genetic variability within the metapopulation. Gene flow seems to counterbalance its effects during the multiannual density fluctuations. The low abundance phase may therefore be too short to impact genetic variation in the host, and consequently viral genetic diversity. Environmental heterogeneity does not seem to affect vole gene flow, which might explain the absence of spatial structure previously detected in PUUV in this area. Besides, our results suggest the role of vole dispersal on PUUV circulation through sex-specific and density-dependent movements. We find little evidence of selection acting on immune-related genes within this metapopulation. Footprint of positive selection is detected atTlr-4gene in 2008 only. We observe marginally significant associations betweenMhc-Drbhaplotypes and PUUV serology, and betweenMx2genotype and PUUV genogroups. These results show that microevolutionary changes and PUUV epidemiology in this metapopulation are mainly driven by neutral processes, although the relative effects of neutral and adaptive forces could vary temporally with density fluctuations.
Perception of stakeholders on corporate social responsibility of Islamic Banks in Jordan
Authors: Di Bella Venere , Al-Fayoumi Nedal 
Published: April 2016

Purpose
– The purpose of this paper is to explore the various perceptions of stakeholders on corporate social responsibility (CSR) of Islamic Banks in Jordan.

Design/methodology/approach
– The data are collected from multiple stakeholder groups of two Islamic Banks in particular: Jordan Islamic Bank for Finance and Investment and Islamic International Arab Bank. The methods adopted to examine the data are the descriptive analysis and analysis of variance. With regard to the purpose of this research, the concept of Islamic CSR and its dimensions have been considered as: rooted in the Islamic ethical system, represented through the profit and loss arrangements, embedded within the principles behind financial services provided by Islamic Banks, and benchmarked by the Accounting and Auditing Organization of Islamic Financial Institutions’ (AAOIFI) corporate governance standard.

Findings
– The results indicate that stakeholders have expressed a positive attitude toward the concept of CSR. Proving that the issue of CSR is an important factor in Islamic banking and to the perception of various stakeholders’ groups, the focus shifted into identifying the dimensions which shape the Islamic CSR. In reference to previous research results, the Islamic banking sector in Jordan has an in-built dimension that promotes social responsibility.

Practical implications
– The study recommends that Islamic Banks improve CSR activities in order to better exploit this commitment with a cultural identity yet again. This identity has a direct influence on the branding of Islamic finance in local markets. The structure of offered products reflects regional beliefs and provides a suite of services. In terms of services, the services provided are geared toward specific market segments within local communities. This as a result directs a number of strategic decisions made by Islamic Banks, which are based on the structure of their offerings, brand identity and customer service levels.

Originality/value
– In Jordan, studies about the perception of stakeholders on CSR from an Islamic perspective are almost non-existent. Thus, providing solutions for study questions and presenting empirical evidence regarding CSR issues will certainly add a new dimension to the literature. Moreover, the conclusions and recommendations may help regulators and decision makers in enhancing the competitiveness and the sustainability of the Islamic banking sector in Jordan.
Enhancing customer loyalty: critical switching cost factors
Authors: El-Manstrly Dahlia 
Published: April 2016

Purpose– The purpose of this paper is to examine the moderator effects of switching costs, classified by type (relational, procedural, and financial) and direction (positive and negative), on the relationships between customer-perceived value, trust, and loyalty.Design/methodology/approach– This study reports on quantitative data from a survey of two service contexts which vary in their degree of customer-employee contact and customization. In total, 360 usable questionnaires were collected, and the data were analyzed using multi-group structural equation modeling.Findings– The results demonstrate that switching costs moderate, in different ways, the relationships between customer loyalty, trust and perceived value. Moreover, the strength of the moderator effects vary according to service type.Research limitations/implications– This study provides new insight into understanding the moderating role of switching costs thus, reduces inconsistencies about the direction and the strength of the moderator effect of switching costs in loyalty frameworks.Practical implications– This study helps managers choose the most effective loyalty strategy for specific service industries and perceptions of switching costs, and to look beyond their service boundaries in order to cross-fertilize strategies for handling switching costs.Originality/value– No empirical study to date has simultaneously examined the moderator effect of switching costs classified by type and direction on the relationships between customer-perceived value, trust, and customer loyalty across two different service contexts in a single framework.
Shopping experiences in visually complex environments: a self-regulation account
Authors: Orth Ulrich R. , Wirtz Jochen , McKinney Amelia 
Published: April 2016

Purpose
– Providing satisfying shopping experiences is a major goal in retail management because satisfaction guides re-patronage behavior. The purpose of this paper is to demonstrate that the visual complexity of an environment’s interior design (i.e. the overall amount of visual information in an environment) influences the shopping experience by impairing customers’ information processing and self-regulation resources.

Design/methodology/approach
– Two quasi-experimental field studies were conducted in two different cultural contexts (i.e. Germany and Singapore) to enhance the external validity and robustness of the findings.

Findings
– Both studies provide evidence that an environment’s visual complexity impairs the shopping experience. Study 1 shows that visual complexity places a perceptual load on customers which mediates the complexity-experience relationship. Study 2 replicates this finding in a different setting and extends it by showing that load relates to lower self-control, which in turn, mars the experience. Furthermore, the negative effect of complexity on the experience is more pronounced with shoppers pursuing utilitarian rather than hedonic shopping goals.

Research limitations/implications
– The findings in a supermarket context may not transfer to environments in which the visual design is an important component of the value proposition and where shopping goals are largely hedonic in nature.

Practical implications
– The findings advance theory by showing that it is perceptual load and its outcome, reduced perceived self-control, which are largely responsible for the negative effect of visual complexity on the shopping experience. This finding should encourage managers to proactively manage and reduce the complexity of their service environments.

Originality/value
– This study is the first to show how the visual complexity of a retail environment influences a customer’s shopping experience. It offers novel insights into the underlying mechanism of perceptual load and self-control as process mediators of visual complexity on the shopping experience.
Value proposition test-driving for service innovation: how frontline employees innovate value propositions
Authors: Åkesson Maria , Skålén Per , Edvardsson Bo , Stålhammar Anna 
Published: April 2016

Purpose
This article investigates the role of frontline employees in service innovation from a service-dominant logic perspective. Frontline employees lack a formal innovation obligation. Service innovation is a resource integration process resulting in the creation of new value propositions.

Design/methodology/approach
A case study of service innovation projects that includes three different businesses in the IT sector and personal interviews with 25 frontline employees.

Findings
The findings suggest that frontline employees contribute to service innovation by test-driving potential value propositions. Three types of value proposition test-driving have been identified: cognitive, practical, and discursive. The findings suggest interdependencies between the different modes of value proposition test-driving, as well as specific phases of the service innovation process dominated by one form or another.

Research limitations/implications
Value proposition test-driving offers a fruitful context for managers to involve frontline employees and use their creativity and expertise. The case study approach, however, limits the statistical generalizability of the findings.

Originality/value
The study is novel in that it (a) introduces the notion of value proposition test-driving for service innovation; (b) provides a systematic empirical analysis of how frontline employees contribute to service innovation by test-driving value propositions; (c) offers a service innovation model informed by the service-dominant logic; and (d) contributes to the service-dominant logic by detailing how service innovation occurs in practice.
Structured selection of partners in open innovation: an IF-TOPSIS based approach
Authors: Aloini Davide , Dulmin Riccardo , Farina Giulia , Mininno Valeria , Pellegrini Luisa 
Published: April 2016

PurposeOpen innovation (OI) literature suggests that firms can improve their innovation performance by learning from a large set of actors in the innovation process. However, the extant literature has overlooked the “who” question: which partners should be included in the different phases of the innovation funnel? How should they be selected? This paper aims to offer, while focusing in the early phases of the innovation process, a list of possible criteria for partner evaluation and suggests a structured methodology for their selection.Design/methodology/approachAn empirical test of both the criteria and the methodology is presented with reference to a company operating in the Advanced Underwater Systems sector. The authors propose a peer-based modification of intuitionistic fuzzy (IF) multi-criteria group decision-making with TOPSIS method (peer IF-TOPSIS). IF-TOPSIS allows coping with subjectivity, imprecision and vagueness in group decision-making problem under multiple criteria.FindingsThe paper proposes an innovative application of a peer-modified version of IF-TOPSIS to a challenging and complex decision problem – partner selection for OI – which is usually subjected to uncertainty and evaluation from multiple experts.Originality/valueThis work contributes to the extant literature advancing the criteria that could be used in the selection process, avoiding focusing on single specific aspects of the collaboration phases or on specific types of partners (suppliers).