bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspectives on how evidence‐based practice changes over time: A qualitative exploration of occupational therapy and physical therapy graduates
Authors: Thomas Aliki , Iqbal Muhammad Zafar , Roberge‐Dao Jacqueline , Ahmed Sara , Bussières André , Debigaré Richard , Letts Lori , MacDermid Joy C. , Paterson Margo , Polatajko Helene J. , Rappolt Susan , Salbach Nancy M. , Rochette Annie 
Published: October 2024

AbstractRationaleThe integration of evidence‐based practice (EBP) into rehabilitation education programs has been widespread, but little is known about how graduates' EBP competencies evolve over time.Aims and ObjectivesTo explore how and why the use of EBP by occupational therapists (OTs) and physical therapists (PTs) evolves during the first 3 years of clinical practice.MethodWe used an interpretive description methodology. We conducted semi‐structured interviews with OTs and PTs who participated in a minimum of three out of four time points in a previous longitudinal pan‐Canadian mixed methods study. Data analysis was guided by Braun and Clarke's approach to thematic analysis.ResultsSeventeen clinicians (13 OTs and 4 PTs) participated in the study. Our analysis identified six overarching themes: (1) evolution of “what EBP is and what it means”; (2) over time, evidence takes a back seat; (3) patients and colleagues have a vital and perennial role in clinical decision making; (4) continuing professional development plays a vital role in EBP; (5) personal attitudes and attributes influence EBP; and (6) organizational factors influence EBP.ConclusionOur study highlights the dynamic nature of EBP use among OTs and PTs in the first 3 years of clinical practice, emphasizing the need for contextualized approaches and ongoing support to promote evidence‐informed healthcare in rehabilitation.
Native and non‐native species response to the colonization and subsequent suppression of northern pike Esox lucius
Authors: Mayer Kent , Garrett Daniel L. , Haukenes Alf H. 
Published: October 2024

AbstractThe expansion of northern Pike Esox lucius outside its native range into the western states of North America is a growing problem due to their negative effects on fish communities. Illegally introduced E. lucius were first detected in Box Canyon Reservoir in northeast Washington in 2004. By 2010, the E. lucius population was estimated at more than 10,000 individuals. Between 2012 and 2019, a gillnet suppression programme was conducted, which reduced the E. lucius catch per unit effort in Box Canyon Reservoir by 97% and their biomass by 98%. Between 2004 and 2019, four standardized surveys were conducted to characterize changes in the Box Canyon Reservoir fish community. Even after E. lucius were effectively suppressed, native species showed no signs of recovery during the study. The relative abundance of all native species decreased 82% in total number and 56% in total biomass between 2004 and 2019. Conversely, the relative abundance of non‐native species increased 26% in total number and 33% in total biomass over the same period. As an apex predator, E. lucius had a significant impact on the fish community in Box Canyon Reservoir over a relatively short period of time (7 years), and appeared to act as a catalyst to increase the rate of replacement of native species with non‐native species, which likely increased the pace of taxonomic homogenization in the reservoir.
Effect of Ultrasound Conditions on Germination and Drying Characteristics of Bengal Gram (Cicer arietinum L.) and Determination of Physicochemical and Functional Properties of Flours
Authors: Choudhury Sumantra , Manind Jhumur , Srivastava Prashant Kumar , Sit Nandan 
Published: October 2024

ABSTRACTThis study aims to investigate the effect of ultrasound conditions on the germination kinetics and drying characteristics of a germinated Bengal gram at different drying temperatures. Ultrasound treatment was given to the Bengal gram seeds at two different conditions, that is, before (US) and after soaking (SU), which was then followed by germination. This study also determines mass transfer parameters at drying temperatures of 45°C, 55°C, and 65°C and assesses the influence on the physicofunctional characteristics of a germinated Bengal gram. The germination rate behavior was effectively predicted using a zero‐order kinetic model with the highest R2 value of 0.7974–0.8857 in each nontreated (S) and treated (SU, US) Bengal gram seed, respectively. This study showed that the ultrasound treatment effectively enhanced the germination rate in both conditions, and the highest germination rate was found in pretreated ultrasound Bengal gram samples. The logarithmic thin layer drying model, with the highest average R2 of 0.9954 and the lowest average RMSE value of 0.0160, is the best‐fitted model to predict the changes in moisture ratio in both treated and nontreated conditions. The moisture diffusivity values at drying temperatures ranging from 45°C to 65°C were found in treated (US, SU) and nontreated (S) germinated samples ranging from 3.34 × 10−8 to 4.03 × 10−8 m2/s, 1.30 × 10−8 to 2.01 × 10−8 m2/s, and 6.6 × 10−9 to 8.06 × 10−9 m2/s, respectively. The protein content increased in the ultrasound‐treated sample between 12.37% and 13.50%. The solubility ranged from 8% to 10.36% throughout the treated and nontreated germinated Bengal gram flour. This study provides a unified approach to utilizing ultrasound‐treated germinated Bengal gram seeds as an alternative option to develop a functional product with better nutritional and functional properties.
Plant Protein Resources, Novel Extraction and Precipitation Methods: A Review
Authors: Akyüz Ayça , Tekin İdil , Aksoy Zülal , Ersus Seda 
Published: October 2024

ABSTRACTThis comprehensive review explores the diverse landscape of plant protein resources, focusing on novel extraction and precipitation methods that contribute to the advancement of sustainable and efficient protein production. The increasing global demand for plant‐based proteins as a key component of balanced diets has spurred research into optimizing extraction processes from various plant sources. The review encompasses a wide range of plant protein resources, including legumes, cereals, oilseeds, and other plant‐based alternatives. Special emphasis is given to innovative techniques in protein extraction, such as enzyme‐assisted extraction, aqueous extraction, and emerging technologies like ultrasound and microwave‐assisted methods. Additionally, the review investigates novel precipitation methods employed to isolate and purify plant proteins, shedding light on techniques like isoelectric precipitation, salting‐out, and membrane filtration. The integration of sustainable practices in protein extraction, coupled with a focus on minimizing environmental impact, is a central theme throughout the review. By synthesizing current research findings, this review aims to provide a comprehensive overview of the state‐of‐the‐art in plant protein extraction and precipitation methods, offering valuable insights for researchers, industry professionals, and policymakers involved in the rapidly evolving field of plant‐based protein production.
The role of antibiotic exposure as risk factor for IBD epidemic: an updated meta‐analysis
Authors: Pan Rui , He Yang , Yuan Jiami , Zhao Shujie , Ma Mengye , Chai Zhenglong , Ji Xinyue , Hu Xinyi , He Canxia , Zhou Dezheng , Zhang Xiaohong 
Published: October 2024

AbstractBackground and AimThe rising prevalence of IBD globally has raised concerns about antibiotic exposure. This study's meta‐analysis examines antibiotic exposure, frequency, year before diagnosis, regional differences, and IBD incidence.MethodsThe literature review used PubMed, Web of Science, Elsevier, ScienceDirect, and Cochrane CENTRAL databases up to June 2024 to explore the link between antibiotic exposure and IBD risk. Stratified analysis was conducted by years of antibiotic exposure before IBD diagnosis, frequency, and region. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were combined using a random effects model.ResultsEighteen case–control studies and five cohort studies were included (n = 99, 104 IBD patients and n = 2 273 336 controls). The findings indicate that antibiotic exposure significantly has a positive association with the risk of developing IBD (OR, 1.66; 95% CI, 1.28–2.16). Antibiotic exposure of ≥3 years (OR, 1.49; 95% CI, 1.12–1.98), 2 years (OR, 1.46; 95% CI, 1.37–1.55), and ≤1 year (OR, 1.55; 95% CI, 1.17–2.04) prior to the diagnosis of IBD is associated with a higher risk of developing IBD. Cumulative exposure of ≥3 dispensations (OR, 2.02; 95% CI, 1.49–2.74) and two dispensations (OR, 1.36; 95% CI, 1.03–1.78) also had a positive association with IBD risk, while one dispensation did not (OR, 0.96; 95% CI, 0.72–1.26). No significant association was found in developing countries (OR, 1.92; 95% CI, 0.71–5.19), but developed countries showed a significant positive association with the risk (OR, 1.58; 95% CI, 1.27–1.96).ConclusionThe meta‐analysis suggests that antibiotic use has a positive association with the risk of IBD, and limiting unnecessary antibiotic use may be one way to reduce the risk of developing IBD.
Predictors of advance care planning in 11 high‐income nations
Authors: Ambade Preshit N. , Hoffman Zachary T. , Mehra Kaamya , MacKinnon Neil J. 
Published: October 2024

AbstractBackgroundElderly population is increasing in high‐income countries. For instance, by 2050, 21.4% of the United States population is expected to be 65+, thus making advance care planning (ACP) increasingly important. We aim to identify predictors of ACP completion in 11 high‐income countries and explore relationships between ACP and utilization factors.MethodUsing the 2021 International Health Policy (IHP) survey data, we assessed the relationship between sociodemographic factors, healthcare utilization, and ACP. The primary outcome variable was a composite of three ACP activities. A generalized linear mixed model (GLMM) was used to identify predictors of ACP completion.ResultsAnalyses included 18,677 older adults who answered at least one ACP question. Only 5126 (27.4%) reported completion of three ACP activities. Germany (64.7%) showed the highest completion rates, while Sweden (5.0%) and France (5.0%) showed the lowest completion rates. Predictors of ACP completion identified in the GLMM were: increasing age (incidence rate ratio [IRR] range between 1.2 and 1.5), completion of high school education or more (IRR: 1.1, 95% CI: 1.1–1.1), higher income (IRR: 1.1, 95% CI: 1.1–1.2), presence of two or more health conditions (IRR: 1.1, 95% CI: 1.0–1.1), hospital stay in the past 2 years (IRR: 1.1, 95% CI: 1.1–1.1), and access to quality primary care (IRR: 1.0, 95% CI: 1.0–1.1). Male gender (IRR: 0.9, 95% CI: 0.8–0.9) had a negative association with ACP activity completion.ConclusionSeveral patient‐specific and health system utilization factors were identified as predictors of ACP activity completion, which clinicians and policymakers could use to enhance ACP completion.
Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48‐linked polyubiquitination in rice
Authors: Cho Lae‐Hyeon , Yoon Jinmi , Baek Gibeom , Tun Win , Kwon Hyeok Chan , Lee Dae‐Woo , Choi Seok‐Hyun , Lee Yang‐Seok , Jeon Jong‐Seong , An Gynheung 
Published: October 2024

ABSTRACTSucrose functions as a signaling molecule in several metabolic pathways as well as in various developmental processes. However, the molecular mechanisms by which sucrose regulates these processes remain largely unknown. In the present study, we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice. Exogenous application of sucrose promoted flowering by inducing florigen gene expression. Reduction of sucrose levels in the phloem through genetic modifications, such as the overexpression of the vacuolar invertase OsVIN2 or the mutation of OsSUT2, a sucrose transporter, delayed flowering. Analysis of relative transcript levels of floral regulatory genes showed that sucrose activated Ehd1 upstream of the florigen, with no significant effect on the expression of other upstream genes. Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded. The Ghd7 protein interacted with the E3 ligase IPA INTERACTING PROTEIN1 (IPI1), and sucrose‐induced K48‐linked polyubiquitination of Ghd7 via IPI1, leading to protein degradation. Mutants defective in IPI1 delayed flowering, confirming its role in modulating proteins involved in flowering. We conclude that sucrose acts as a signaling molecule to induce flowering by promoting Ghd7 degradation via IPI1.
Activation of ionotropic and group I metabotropic glutamate receptors stimulates kisspeptin neuron activity in mice
Authors: Bearss Robin J. , Oliver Isabella A. , Neuman Peighton N. , Abdulmajeed Wahab I. , Ackerman Jennifer M. , Piet Richard 
Published: October 2024

AbstractDifferent populations of hypothalamic kisspeptin (KISS1) neurons located in the rostral periventricular area of the third ventricle (RP3V) and arcuate nucleus (ARC) are thought to generate the sex‐specific patterns of gonadotropin secretion. These neuronal populations integrate gonadal sex steroid feedback with internal and external cues relayed via the actions of neurotransmitters and neuropeptides. The excitatory amino acid neurotransmitter glutamate, the main excitatory neurotransmitter in the brain, plays a role in regulating gonadotropin secretion, at least partially through engaging KISS1 signaling. The expression and function of individual glutamate receptor subtypes in KISS1 neurons, however, are not well characterized. Here, we used GCaMP‐based calcium imaging and patch‐clamp electrophysiology to assess the impact of activating individual ionotropic (iGluR) and group I metabotropic (mGluR) glutamate receptors on KISS1 neuron activity in the mouse RP3V and ARC. Our results indicate that activation of all iGluR subtypes and of group I mGluRs, likely mGluR1, consistently drives activity in the majority of KISS1 neurons within the RP3V and ARC of males and females. Our results also revealed, somewhat unexpectedly, sex‐ and region‐specific differences. Indeed, activating (S)‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) type iGluRs evoked larger responses in female ARCKISS1 neurons than in their male counterparts whereas activating group I mGluRs induced larger responses in RP3VKISS1 neurons than in ARCKISS1 neurons in females. Together, our findings suggest that glutamatergic neurotransmission in KISS1 neurons, and its impact on the activity of these cells, might be sex‐ and region‐dependent in mice.
Everyday State Attachment: Dynamic Features and Role of Trait Attachment
Authors: Tammilehto Jaakko , Kaurin Aleksandra , Bosmans Guy , Kuppens Peter , Flykt Marjo , Vänskä Mervi , Peltonen Kirsi , Lindblom Jallu 
Published: October 2024

ABSTRACTObjectiveAttachment research has traditionally focused on traits, enhancing our understanding of attachment‐related individual differences. However, to chart the dynamic properties of the attachment system, more research is needed on the within‐person fluctuation of attachment states. In this ecological momentary assessment (EMA) study, we examined (a) the associations between the baseline, variability, and inertia of each state attachment dimension (security, avoidance, and anxiety) and (b) how trait attachment (anxiety and avoidance) predicts these dynamic features.MethodIn two adult samples (Ns = 122 and 127), trait attachment dimensions were first assessed using Experiences in Close Relationships–Revised. Then, attachment states were assessed seven or ten times a day over 1 week (4629 and 5322 successful EMA observations).ResultsFor state security, individuals with high baseline exhibited lower variability. In contrast, for state avoidance, those with high baseline showed higher variability. Both trait attachment anxiety and avoidance predicted lower baseline and higher variability of state security. Moreover, both trait dimensions predicted higher baselines of the corresponding states.ConclusionsOur findings provide insights into the real‐time regulatory dynamics of the attachment system and their interconnection with trait attachment, underscoring the importance of baseline and variability in understanding how attachment manifests in everyday life.
From Seasonality to Species Conservation: Chronobiological Research on European Hamsters in Strasbourg, France
Authors: Monecke Stefanie 
Published: October 2024

ABSTRACTThe first monograph on the European hamster from the Strasbourg region dates back to 1765. By the 1930s, a long and continuous chronobiological research tradition was established for this species, starting with the works of Charles Kayser, who published between 1938 and 1971. Another early key researcher in this area was Bernhard Canguilhem with publications from 1966 to 1999. From the 1980s onwards, “the Pévets,” Paul Pévet and his wife, Mireille Masson‐Pévet, gave new energy to European hamster research. They broadened the research scope from basic hibernation research to mechanistic studies of circannual rhythms and from physiological aspects to molecular details. One main underlying question in their research was the role of melatonin. Thanks to their enthusiasm and vision, the European hamster is today one of the best – if not the best – studied circannual species. At least 73 parameters are described to cycle. Thirty‐two of them have been shown to be driven by a circannual clock. Moreover, ground‐breaking advances in our understanding of the mechanistic of hibernation, circannual clock functioning, and its entrainment were made. With most of this research being conducted in Strasbourg, Paul Pévet was instrumental in providing the necessary resources that made these innovative and unconventional long‐term animal studies possible, contributing to fundamental research and, ultimately, to species conservation.
Resting cortisol concentrations in dogs presenting to a university teaching hospital with collapse
Authors: Fernandez Gallego Ana , Breheny Craig Robert , Gow Adam G. , Boag Alisdair M. 
Published: October 2024

AbstractBackgroundThe relationship between collapse and a diagnosis of hypoadrenocorticism is not well understood in dogs.HypothesisTo assess the prevalence of episodes of collapse in dogs screened for hypoadrenocorticism, and to assess the prevalence of confirmed hypoadrenocorticism in dogs presenting with reported collapse.AnimalsSeventy‐three client‐owned dogs with resting cortisol concentrations were measured and presented to a University teaching hospital for collapse.MethodsRetrospective review of medical records of dogs at a single center.ResultsThe prevalence of episodes of collapse in dogs that had a resting cortisol measurement was 73/856 (8.5%; 95% confidence interval [95% CI], 6.7%‐10.6%). Resting cortisol concentration was <2 μg/dL (<55 nmol/L) in 19 dogs. Cortisol concentration after ACTH stimulation was <2 μg/dL (<55 nmol/L) in 1 of the 73 dogs in this cohort, consistent with a diagnosis of hypoadrenocorticism, giving a prevalence estimate of hypoadrenocorticism of 1.3% (95% CI, 0.15%‐6.2%). In 8 dogs with an initial resting cortisol concentration <2 μg/dL (<55 nmol/L), hypoadrenocorticism was excluded based on a repeat resting cortisol concentration >2 μg/dL (>55 nmol/L). The most common diagnosis was vasovagal syncope (10/73), followed by sick sinus syndrome and third‐degree atrioventricular block (2/73). The final diagnosis was unknown in 24/73 dogs.Conclusions and Clinical ImportanceHypoadrenocorticism was the final diagnosis in 1 of 73 dogs presented to a teaching hospital either in a collapsed state or with a previous history of episodes of collapse. No dog presenting as cardiovascularly stable for intermittent collapse was found to have hypoadrenocorticism.
Plant‐to‐plant defence induction in cotton is mediated by delayed release of volatiles upon herbivory
Authors: Grandi Luca , Ye Wenfeng , Clancy Mary V. , Vallat Armelle , Glauser Gaétan , Abdala‐Roberts Luis , Brevault Thierry , Benrey Betty , Turlings Ted C. J. , Bustos‐Segura Carlos 
Published: October 2024

Summary
Caterpillar feeding immediately triggers the release of volatile compounds stored in the leaves of cotton plants. Additionally, after 1 d of herbivory, the leaves release other newly synthesised volatiles. We investigated whether these volatiles affect chemical defences in neighbouring plants and whether such temporal shifts in emissions matter for signalling between plants.
Undamaged receiver plants were exposed to volatiles from plants infested with Spodoptera caterpillars. For receiver plants, we measured changes in defence‐related traits such as volatile emissions, secondary metabolites, phytohormones, gene expression, and caterpillar feeding preference. Then, we compared the effects of volatiles emitted before and after 24 h of damage on neighbouring plant defences.
Genes that were upregulated in receiver plants following exposure to volatiles from damaged plants were the same as those activated directly by herbivory on a plant. Only volatiles emitted after 24 h of damage, including newly produced volatiles, were found to increase phytohormone levels, upregulate defence genes, and enhance resistance to caterpillars.
These results indicate that the defence induction by volatiles is a specific response to de novo synthesised volatiles, suggesting that these compounds are honest signals of herbivore attack. These findings point to an adaptive origin of airborne signalling between plants.
Application of Modified Skin Stretching for Soft Tissue Defect Reconstruction in the Ankle and Foot: A Retrospective Report
Authors: Yang Xiaqing , Liu Yuping , Wang Weixing , Fang Xue , Zhang Wang , Liu Changhuan , Wang Xin 
Published: October 2024

ABSTRACTObjectiveThe failure rate of foot and ankle soft tissue defect reconstruction with flap is relatively high, often posing a significant burden on patients. The aim of this study is to explore the effectiveness of repeated stretch sutures in repairing skin and soft tissue defects of the ankle and foot.MethodsTwenty‐three patients with ankle and foot skin and soft tissue defects were retrospectively analyzed between February 2016 and February 2019. Sutures were repeatedly stretched every 3–5 days. Local skin grafting was performed if necessary after wound surfaces disappeared or exposed tendons and bones were covered by soft tissue. Wound healing time, postoperative healing area, Vancouver Scar Assessment Scale, sensation, and function of the new skin were evaluated.ResultsHealing time was 17–35 (24.43 ± 5.29) days. Ten patients wholly healed, and 13 healed by approximately 70.08% ± 6.59%. The Vancouver Scar Assessment Scale average score was 2.83 ± 1.19 points, of which 15 cases were excellent (0–3 points) and 8 cases were good (4–7 points). The sensation and function of the new skin after repair were equivalent to those of normal skin after the last follow‐up.ConclusionsApplying repeated tension sutures on the skin and soft defects of the ankle and foot reduced the skin graft area and decreased complex high‐risk surgical flaps' use and transplantation area.