bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lost and found: the rediscovery of the lost fern species Asplenium achalense (Aspleniaceae) and assessment of its conservation status
Authors: Arana Marcelo , Natale Evangelina , Oggero Antonia 
Published: September 2024

AbstractThe fern of Achala Asplenium achalense Hieron. (Aspleniaceae), endemic to north-west and central Argentina, was formerly considered a lost species. We describe its rediscovery in August 2022 in the Yungas biogeographical province, compile a map of all historical and current records of the species, and propose an IUCN Red List status. We estimated the fern of Achala's range using the area of occupancy of the rediscovered population, calculated as 4 km2 because it is located in a single 2 × 2 km grid square. The extent of occurrence cannot be calculated because only a single living population is known. These data suggest a provisional categorization of the fern of Achala as Critically Endangered based on criteria B2ab(i,ii,iii,iv,v). The species should be considered Regionally Extinct in its locus classicus in Comechingones biogeographical province. We recommend securing the conservation of the single known population (which does not lie within a conservation unit), further surveys for the species in the five protected areas where it was recorded historically, and restoration of the species in the wild. To support the latter, we are cultivating spores and gametophytes of the fern of Achala, in collaboration with colleagues from the National University of La Plata.
Evidence for a floristically diverse rainforest on the Falkland archipelago in the remote South Atlantic during the mid- to late Cenozoic
Authors: Thomas Zoë A. , Macphail Michael , Cadd Haidee , Cantrill David J. , Hutchinson David K. , Haines Heather A. , Privat Karen , Turney Chris , Carter Stefanie , Brickle Paul 
Published: September 2024

AbstractWe report the discovery of an ancient forest bed near Stanley, on the Falkland Islands, the second such ancient deposit identified on the South Atlantic island archipelago that is today marked by the absence of native tree species. Fossil pollen, spores and wood fragments preserved in this buried deposit at Tussac House show that the source vegetation was characterized by a floristically diverse rainforest dominated by Nothofagus-Podocarpaceae communities, similar to cool temperate Nothofagus forests/woodlands and Magellanic evergreen Nothofagus rainforests. The age limit of the deposit is inferred from the stratigraphic distribution of fossil pollen species transported by wind, birds or ocean currents from southern Patagonia, as well as similar vegetation types observed across the broader region. The deposit is suggested to be between Late Oligocene and Early Miocene, making it slightly older than the previously analysed Neogene West Point Island forest bed (200 km west of Tussac House). The combined evidence adds to our current knowledge of the role of climate change and transoceanic dispersal of plant propagules in shaping high-latitude ecosystems in the Southern Hemisphere during the late Palaeogene and Neogene.
Analytic Cognition in Kant
Authors: Yuen Michael 
Published: September 2024

AbstractKant refers to analytic cognition in several prominent places. The prevailing wisdom, however, denies the possibility of analytic cognition within his theory of cognition. I shall argue that this is mistaken. I show that we can account for analytic cognition’s possibility by appealing to variants of the more familiar conditions on the cognition of objects. I also highlight analytic cognition’s connection to insight and analytic knowledge. In the process, I provide a fuller account of Kant’s view of our mental lives than has been typically acknowledged.
Antecedent Hydrologic Conditions Reflected in Stream Lithium Isotope Ratios During Storms
Authors: Golla Jon K. , Bouchez Julien , Druhan Jennifer L. 
Published: September 2024

AbstractAntecedent hydrological conditions are recorded through the evolution of dissolved lithium isotope signatures (Li) by juxtaposing two storm events in an upland watershed subject to a Mediterranean climate. Discharge and Li are negatively correlated in both events, but mean Li ratios and associated ranges of variation are distinct between them. We apply a previously developed reactive transport model (RTM) for the site to these event‐scale flow perturbations, but observed shifts in stream Li are not reproduced. To reconcile the stability of the subsurface solute weathering profile with our observations of dynamic stream Li signatures, we couple the RTM to a distribution of fluid transit times that evolve based on storm hydrographs. The approach guides appropriate flux‐weighting of fluid from the RTM over a range of flow path lengths, or equivalently fluid residence times. This flux‐weighted RTM approach accurately reproduces dynamic storm Li‐discharge patterns distinguished by the antecedent conditions of the watershed.
Anti-PD-L1 antibody ASC22 in combination with a histone deacetylase inhibitor chidamide as a “shock and kill” strategy for ART-free virological control: a phase II single-arm study
Authors: Wu Luling , Zheng Zhihang , Xun Jingna , Liu Li , Wang Jiangrong , Zhang Xinyu , Shao Yueming , Shen Yinzhong , Zhang Renfang , Zhang Min , Sun Meiyan , Qi Tangkai , Wang Zhenyan , Xu Shuibao , Song Wei , Tang Yang , Zhao Bihe , Song Zichen , Routy Jean-Pierre , Lu Hongzhou , Chen Jun 
Published: September 2024

AbstractThe combination of ASC22, an anti-PD-L1 antibody potentially enhancing HIV-specific immunity and chidamide, a HIV latency reversal agent, may serve as a strategy for antiretroviral therapy-free virological control for HIV. People living with HIV, having achieved virological suppression, were enrolled to receive ASC22 and chidamide treatment in addition to their antiretroviral therapy. Participants were monitored over 24 weeks to measure changes in viral dynamics and the function of HIV-specific CD8+ T cells (NCT05129189). 15 participants completed the study. At week 8, CA HIV RNA levels showed a significant increase from baseline, and the values returned to baseline after discontinuing ASC22 and chidamide. The total HIV DNA was only transiently increased at week 4 (P = 0.014). In contrast, integrated HIV DNA did not significantly differ from baseline. Increases in the proportions of effector memory CD4+ and CD8+ T cells (TEM) were observed from baseline to week 24 (P = 0.034 and P = 0.002, respectively). The combination treatment did not succeed in enhancing the function of HIV Gag/Pol- specific CD8+ T cells. Nevertheless, at week 8, a negative correlation was identified between the proportions of HIV Gag-specific TEM cells and alterations in integrated DNA in the T cell function improved group (P = 0.042 and P = 0.034, respectively). Nine adverse events were solicited, all of which were graded 1 and resolved spontaneously. The combined treatment of ASC22 and chidamide was demonstrated to be well-tolerated and effective in activating latent HIV reservoirs. Further investigations are warranted in the context of analytic treatment interruption.
Cardiovascular adverse effects of antiviral therapies for COVID-19: Evidence and plausible mechanisms
Authors: Chen Eileen , Xi Lei 
Published: September 2024

Abstract
Antiviral therapeutics have made a critical contribution in mitigating the symptoms and clinical outcomes of the coronavirus disease of 2019 (COVID-19), in which a single-stranded RNA viral pathogen, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes multi-organ injuries. Several antivirals were widely prescribed to treat COVID-19, either through the emergency use authorization (EUA) by the governmental regulatory agencies (i.e., remdesivir, paxlovid, molnupiravir, and the SARS-CoV-2-targeted monoclonal antibodies - tixagevimab and cilgavimab), as well as the repurposed use of the existing antiviral or antimalarial drugs (e.g., hydroxychloroquine, chloroquine, and ivermectin). Despite their efficacy in ameliorating COVID-19 symptoms, some adverse side-effects of the antivirals were also reported during the COVID-19 pandemic. Our current review has aimed to gather and extrapolate the recently published information concerning cardiovascular adverse effects caused by each of the antivirals. We also provide further discussion on the potential cellular mechanisms underlying the cardiovascular adverse effects of the selected antiviral drugs, which should be carefully considered when evaluating risk factors in managing patients with COVID-19 or similar infectious diseases. It is foreseeable that future antiviral drug development assisted with the newest artificial intelligence platform may improve the accuracy to predict the structures of biomolecules of antivirals and therefore to mitigate their associated cardiovascular adversities.
Cilia defects upon loss of WDR4 are linked to proteasomal hyperactivity and ubiquitin shortage
Authors: Burkhalter Martin D. , Stiff Tom , Maerz Lars D. , Casar Tena Teresa , Wiese Heike , Gerhards Julian , Sailer Steffen A. , Vu Linh Anna Trúc , Duong Phu Max , Donow Cornelia , Alupei Marius , Iben Sebastian , Groth Marco , Wiese Sebastian , Church Joseph A. , Jeggo Penelope A. , Philipp Melanie 
Published: September 2024

AbstractThe WD repeat-containing protein 4 (WDR4) has repeatedly been associated with primary microcephaly, a condition of impaired brain and skull growth. Often, faulty centrosomes cause microcephaly, yet aberrant cilia may also be involved. Here, we show using a combination of approaches in human fibroblasts, zebrafish embryos and patient-derived cells that WDR4 facilitates cilium formation. Molecularly, we associated WDR4 loss-of-function with increased protein synthesis and concomitant upregulation of proteasomal activity, while ubiquitin precursor pools are reduced. Inhibition of proteasomal activity as well as supplementation with free ubiquitin restored normal ciliogenesis. Proteasome inhibition ameliorated microcephaly phenotypes. Thus, we propose that WDR4 loss-of-function impairs head growth and neurogenesis via aberrant cilia formation, initially caused by disturbed protein and ubiquitin homeostasis.
FoxO transcription factors actuate the formative pluripotency specific gene expression programme
Authors: Santini Laura , Kowald Saskia , Cerron-Alvan Luis Miguel , Huth Michelle , Fabing Anna Philina , Sestini Giovanni , Rivron Nicolas , Leeb Martin 
Published: September 2024

AbstractNaïve pluripotency is sustained by a self-reinforcing gene regulatory network (GRN) comprising core and naïve pluripotency-specific transcription factors (TFs). Upon exiting naïve pluripotency, embryonic stem cells (ESCs) transition through a formative post-implantation-like pluripotent state, where they acquire competence for lineage choice. However, the mechanisms underlying disengagement from the naïve GRN and initiation of the formative GRN are unclear. Here, we demonstrate that phosphorylated AKT acts as a gatekeeper that prevents nuclear localisation of FoxO TFs in naïve ESCs. PTEN-mediated reduction of AKT activity upon exit from naïve pluripotency allows nuclear entry of FoxO TFs, enforcing a cell fate transition by binding and activating formative pluripotency-specific enhancers. Indeed, FoxO TFs are necessary and sufficient for the activation of the formative pluripotency-specific GRN. Our work uncovers a pivotal role for FoxO TFs in establishing formative post-implantation pluripotency, a critical early embryonic cell fate transition.
MerlinS13 phosphorylation regulates meningioma Wnt signaling and magnetic resonance imaging features
Authors: Eaton Charlotte D. , Avalos Lauro , Liu S. John , Chen Zhenhong , Zakimi Naomi , Casey-Clyde Tim , Bisignano Paola , Lucas Calixto-Hope G. , Stevenson Erica , Choudhury Abrar , Vasudevan Harish N. , Magill Stephen T. , Young Jacob S. , Krogan Nevan J. , Villanueva-Meyer Javier E. , Swaney Danielle L. , Raleigh David R. 
Published: September 2024

AbstractMeningiomas are associated with inactivation of NF2/Merlin, but approximately one-third of meningiomas with favorable clinical outcomes retain Merlin expression. Biochemical mechanisms underlying Merlin-intact meningioma growth are incompletely understood, and non-invasive biomarkers that may be used to guide treatment de-escalation or imaging surveillance are lacking. Here, we use single-cell RNA sequencing, proximity-labeling proteomic mass spectrometry, mechanistic and functional approaches, and magnetic resonance imaging (MRI) across meningioma xenografts and patients to define biochemical mechanisms and an imaging biomarker that underlie Merlin-intact meningiomas. We find Merlin serine 13 (S13) dephosphorylation drives meningioma Wnt signaling and tumor growth by attenuating inhibitory interactions with β-catenin and activating the Wnt pathway. MRI analyses show Merlin-intact meningiomas with S13 phosphorylation and favorable clinical outcomes are associated with high apparent diffusion coefficient (ADC). These results define mechanisms underlying a potential imaging biomarker that could be used to guide treatment de-escalation or imaging surveillance for patients with Merlin-intact meningiomas.
Biomethane produced from maize grown on peat emits more CO2 than natural gas
Authors: Evans Chris D. , Rowe Rebecca L. , Freeman Benjamin W. J. , Rhymes Jennifer M. , Cumming Alex , Lloyd Isobel L. , Morton Daniel , Williamson Jennifer L. , Morrison Ross 
Published: September 2024

AbstractCultivation of maize for biomethane production has expanded rapidly, including on drained peat soils. The resulting soil CO2 emissions at the point of feedstock production are largely overlooked when assessing biogas climate mitigation potential. On the basis of field-scale flux measurements, we calculate that soil CO2 emissions from biomethane feedstock production on drained peat exceed embodied emissions for an equivalent amount of natural gas by up to a factor of three.
DNA microbeads for spatio-temporally controlled morphogen release within organoids
Authors: Afting Cassian , Walther Tobias , Drozdowski Oliver M. , Schlagheck Christina , Schwarz Ulrich S. , Wittbrodt Joachim , Göpfrich Kerstin 
Published: September 2024

AbstractOrganoids are transformative in vitro model systems that mimic features of the corresponding tissue in vivo. However, across tissue types and species, organoids still often fail to reach full maturity and function because biochemical cues cannot be provided from within the organoid to guide their development. Here we introduce nanoengineered DNA microbeads with tissue mimetic tunable stiffness for implementing spatio-temporally controlled morphogen gradients inside of organoids at any point in their development. Using medaka retinal organoids and early embryos, we show that DNA microbeads can be integrated into embryos and organoids by microinjection and erased in a non-invasive manner with light. Coupling a recombinant surrogate Wnt to the DNA microbeads, we demonstrate the spatio-temporally controlled morphogen release from the microinjection site, which leads to morphogen gradients resulting in the formation of retinal pigmented epithelium while maintaining neuroretinal cell types. Thus, we bioengineered retinal organoids to more closely mirror the cell type diversity of in vivo retinae. Owing to the facile, one-pot fabrication process, the DNA microbead technology can be adapted to other organoid systems for improved tissue mimicry.
Golden eagle optimized fractional-order PI controller design for a PFC SEPIC converter in EV charging
Authors: Vijayakumar S. , Sudhakar N. 
Published: September 2024

AbstractThe power factor correction converter is the function of the front-end converter, followed by the DC–DC converter of the electric vehicle charger. It improves the power factor and regulates the output voltage and current. This research article proposes the Golden Eagle optimization for fractional order PI (FOPI) controller for Single Ended Primary Inductor Converter (SEPIC) power factor correction. The Golden eagle optimization is based on its knowledge of hunting tactics at various degrees of spiral trajectories to catch the prey. The FOPI controller has a broad range of controller parameters that provide better control and performance of the converter. The tuning of the parameters of the FOPI controller is optimized in Golden Eagle Optimization, and the Integral Absolute error with Integral Square error is used for the objective function. The optimized parameters of FOPI compare with the conventional PI controller performance. The SEPIC converter is designed and derived from the state space model by state space averaging, and the reduced model is obtained through the moment matching method. This system is tested under MATLAB/SIMULINK, and simulation results show improved settling time, fast dynamic response, reduction of inrush current, less harmonic distortion, and stability.
Microbial dynamics, chemical profile, and bioactive potential of diverse Egyptian marine environments from archaeological wood to soda lake
Authors: Hegazy Ghada E. , Moawad Madelyn N. , Othman Sarah Samir , Soliman Nadia A. , Abeer E Abdelwahab , Oraby Hussein , Abdel–Fattah Yasser R. 
Published: September 2024

AbstractHalophilic archaea are a unique group of microorganisms that thrive in high–salt environments, exhibiting remarkable adaptations to survive extreme conditions. Archaeological wood and El–Hamra Lake serve as a substrate for a diverse range of microorganisms, including archaea, although the exact role of archaea in archaeological wood biodeterioration remains unclear. The morphological and chemical characterizations of archaeological wood were evaluated using FTIR, SEM, and EDX. The degradation of polysaccharides was identified in Fourier transform infrared analysis (FTIR). The degradation of wood was observed through scanning electron microscopy (SEM). The energy dispersive X–ray spectroscopy (EDX) revealed the inclusion of minerals, such as calcium, silicon, iron, and sulfur, into archaeological wood structure during burial and subsequent interaction with the surrounding environment. Archaea may also be associated with detected silica in archaeological wood since several organosilicon compounds have been found in the crude extracts of archaeal cells. Archaeal species were isolated from water and sediment samples from various sites in El–Hamra Lake and identified as Natronococcus sp. strain WNHS2, Natrialba hulunbeirensisstrain WNHS14, Natrialba chahannaoensis strain WNHS9, and Natronococcus occultus strain WNHS5. Additionally, three archaeal isolates were obtained from archaeological wood samples and identified as Natrialba chahannaoensisstrain W15, Natrialba chahannaoensisstrain W22, and Natrialba chahannaoensisstrain W24. These archaeal isolates exhibited haloalkaliphilic characteristics since they could thrive in environments with high salinity and alkalinity. Crude extracts of archaeal cells were analyzed for the organic compounds using gas chromatography–mass spectrometry (GC–MS). A total of 59 compounds were identified, including free saturated and unsaturated fatty acids, saturated fatty acid esters, ethyl and methyl esters of unsaturated fatty acids, glycerides, phthalic acid esters, organosiloxane, terpene, alkane, alcohol, ketone, aldehyde, ester, ether, and aromatic compounds. Several organic compounds exhibited promising biological activities. FTIR spectroscopy revealed the presence of various functional groups, such as hydroxyl, carboxylate, siloxane, trimethylsilyl, and long acyl chains in the archaeal extracts. Furthermore, the archaeal extracts exhibited antioxidant effects. This study demonstrates the potential of archaeal extracts as a valuable source of bioactive compounds with pharmaceutical and biomedical applications.
Trends of Toxoplasma gondii and common transfusable venereal infections among blood donors in Menoufia Province, Egypt
Authors: Gouda Marwa A. , Saied Sara A. , Ashry Walaa Mohamed Omar , Abd-Eltwab Raafat Abd-Rabow , Aldesoky Mohamed Morshdy , El-dydamoni Omnia Ahmed , Yousef Marwa , El-Derbawy Mona M. 
Published: September 2024

AbstractBlood transfusion has a hazard of transmission of many pathogens, including Toxoplasma gondii (T. gondii) and other venereal infections. It is crucial to conduct epidemiological surveillance to detect the prevalence of these pathogens. The study aimed to assess the seroprevalence of T. gondii and common transfusable venereal infections among healthy blood donors in Menoufia Province, Egypt, and identify associated risk factors. Four hundred twenty individuals were recruited between January and April 2023 for cross-sectional descriptive research from the blood banks of Menoufia University medical hospitals. Collected blood samples were screened for anti-T. gondii IgM and IgG, HBsAg, anti-HCV antibodies, HIV p24 antigen and anti-HIV antibodies, and anti-Treponema pallidum antibodies. 46 (11.0%) and 22 donors (5.2%) individuals tested positive for anti-T. gondii IgG with a 95% CI (8.3–14.6) and IgM with a 95% CI (3.5–8.1), respectively, while one patient (0.2%) was positive for both antibodies. Regarding venereal infections, 12 (2.9%) were positive for HBV, 6 (1.4%) were positive for HCV, 7 (1.7%) were positive for HIV, and none of the tested population showed positivity for syphilis. Female gender, consumption of raw meat, agricultural environment, poor awareness about T. gondii, and blood group type (especially AB and O groups) were identified as independent risk factors for T. gondii infection. The study highlights the importance of testing blood donors for T. gondii and common transfusable venereal illnesses. Starting health education programs and preventative measures, such as suitable meat handling and cleanliness practices, is critical for minimizing the occurrence of these illnesses. Larger-scale additional study is advised to confirm these results and provide guidance for public health initiatives.
Bipolar electrochemical growth of conductive microwires for cancer spheroid integration: a step forward in conductive biological circuitry
Authors: Robinson Andie J. , McBeth Craig , Rahman Ruman , Hague Richard J. M. , Rawson Frankie J. 
Published: September 2024

AbstractThe field of bioelectronics is developing exponentially. There is now a drive to interface electronics with biology for the development of new technologies to improve our understanding of electrical forces in biology. This builds on our recently published work in which we show wireless electrochemistry could be used to grow bioelectronic functional circuitry in 2D cell layers. To date our ability to merge electronics with in situ with biology is 3D limited. In this study, we aimed to further develop the wireless electrochemical approach for the self-assembly of microwires in situ with custom-designed and fabricated 3D cancer spheroids. Unlike traditional electrochemical methods that rely on direct electrical connections to induce currents, our technique utilises bipolar electrodes that operate independently of physical wired connections. These electrodes enable redox reactions through the application of an external electric field. Specifically, feeder electrodes connected to a power supply generate an electric field, while the bipolar electrodes, not physically connected to the feeder electrodes, facilitate the reduction of silver ions from the solution. This process occurs upon applying a voltage across the feeder electrodes, resulting in the formation of self-assembled microwires between the cancer spheroids.Thereby, creating interlinked bioelectronic circuitry with cancer spheroids. We demonstrate that a direct current was needed to stimulate the growth of conductive microwires in the presence of cell spheroids. Microwire growth was successful when using 50 V (0.5 kV/cm) of DC applied to a single spheroid of approximately 800 µm in diameter but could not be achieved with alternating currents. This represents the first proof of the concept of using wireless electrochemistry to grow conductive structures with 3D mammalian cell spheroids.