bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macrophage-Expressed Coagulation Factor VII Promotes Adverse Cardiac Remodeling
Authors: Garlapati Venkata , Luo Qi , Posma Jens , Aluia Melania , Nguyen Than Son , Grunz Kristin , Molitor Michael , Finger Stefanie , Harms Gregory , Bopp Tobias , Ruf Wolfram , Wenzel Philip 
Published: September 2024

BACKGROUND:
Excess fibrotic remodeling causes cardiac dysfunction in ischemic heart disease, driven by MAP (mitogen-activated protein) kinase–dependent TGF-ß1 (transforming growth factor-ß1) activation by coagulation signaling of myeloid cells. How coagulation-inflammatory circuits can be specifically targeted to achieve beneficial macrophage reprogramming after myocardial infarction (MI) is not completely understood.

METHODS:
Mice with permanent ligation of the left anterior descending artery were used to model nonreperfused MI and analyzed by single-cell RNA sequencing, protein expression changes, confocal microscopy, and longitudinal monitoring of recovery. We probed the role of the tissue factor (TF)-FVIIa (activated factor VII)-integrin ß1-PAR2 (protease-activated receptor 2) signaling complex by utilizing genetic mouse models and pharmacological intervention.

RESULTS:

Cleavage-insensitive PAR2
R38E
and myeloid cell integrin ß1–deficient mice had improved cardiac function after MI compared with controls. Proximity ligation assays of monocytic cells demonstrated that colocalization of FVIIa with integrin ß1 was diminished in monocyte/macrophage FVII–deficient mice after MI. Compared with controls, F7
fl/fl
CX3CR1 (CX3C motif chemokine receptor 1)
Cre
mice showed reduced TGF-ß1 and MAP kinase activation, as well as cardiac dysfunction after MI, despite unaltered overall recruitment of myeloid cells. Single-cell mRNA sequencing of CD45 (cluster of differentiation 45)
+
cells 3 and 7 days after MI uncovered a trajectory from recruited monocytes to inflammatory TF
+
/TREM (triggered receptor expressed on myeloid cells) 1
+
macrophages requiring F7. As early as 7 days after MI, macrophage F7 deletion led to an expansion of reparative Olfml 3 (olfactomedin-like protein 3)
+
macrophages and, conversely, to a reduction of TF
+
/TREM1
+
macrophages, which were also reduced in PAR2
R38E
mice. Short-term treatment from days 1 to 5 after nonreperfused MI with a monoclonal antibody inhibiting the macrophage TF-FVIIa-PAR2 signaling complex without anticoagulant activity improved cardiac dysfunction, decreased excess fibrosis, attenuated vascular endothelial dysfunction, and increased survival 28 days after MI.

CONCLUSIONS:
Extravascular TF-FVIIa-PAR2 complex signaling drives inflammatory macrophage polarization in ischemic heart disease. Targeting this signaling complex for specific therapeutic macrophage reprogramming following MI attenuates cardiac fibrosis and improves cardiovascular function.
Blood Pressure–Lowering Medications, Sodium Reduction, and Blood Pressure
Authors: Song Jing , Chen Liangkai , Xiong Hui , Ma Yuan , Pombo-Rodrigues Sonia , MacGregor Graham A. , He Feng J. 
Published: September 2024

BACKGROUND:
Both blood pressure–lowering medication and sodium reduction are effective in hypertension control, but whether the effect of sodium reduction differ across blood pressure–lowering medications is unclear. This study aims to evaluate the dose-response effect of sodium intake reduction on blood pressure in treated hypertensive individuals and the impact of different classes of blood pressure–lowering drugs.

METHODS:
We searched multiple databases and reference lists up to July 9, 2024. Randomized controlled trials with a duration of ≥2 weeks comparing the effect of different levels of sodium intake (measured by 24-hour urinary sodium excretion) on blood pressure in hypertensive individuals treated with constant blood pressure–lowering medications were included. Instrumental variable meta-analyses based on random-effects models were conducted to evaluate the dose effect of sodium reduction on blood pressure. Subgroup analyses were performed based on the class of blood pressure–lowering drugs, age, baseline sodium and blood pressure levels, and study duration.

RESULTS:
We included 35 studies (median duration of 28 days) with a total of 2885 participants. For every 100 mmol reduction in 24-hour urinary sodium excretion, systolic blood pressure decreased by 6.81 mm Hg (95% CI, 4.96–8.66), diastolic blood pressure decreased by 3.85 mm Hg (95% CI, 2.26–5.43), and mean arterial pressure decreased by 4.83 mm Hg (95% CI, 3.22–6.44). The dose-response effects varied across classes of blood pressure–lowering medications, with greater effects observed in the β-blockers, renin-angiotensin-aldosterone system inhibitors, and dual therapy groups. No significant subgroup differences were observed across subgroups defined by age, baseline 24-hour urinary sodium excretion, blood pressure levels, or study duration.

CONCLUSIONS:
Pooled evidence suggests a dose-response relationship between sodium reduction and blood pressure in treated individuals with hypertension, influenced by the class of blood pressure–lowering medications.
Cardiovascular Risks and Outcomes Among Chinese American Immigrants: Insights From the Multi‐Ethnic Study of Atherosclerosis
Authors: Cai Xinjiang , White Quinn , Wang Daniel R. , DeFilippi Christopher R. , Bertoni Alain G. , Wu Colin O. , Liu Kiang , Lima Joao A. C. , Budoff Matthew J. , Fonarow Gregg C. , Watson Karol E. , McClelland Robyn L. , Yang Eric H. 
Published: September 2024

Background
Immigrants experience changes in cardiovascular risk factors and racial disparities in both cardiovascular health prevention and outcomes upon immigration. We aimed to examine cardiovascular risk factors and outcomes among Chinese American immigrants enrolled in the MESA (Multi‐Ethnic Study of Atherosclerosis) cohort.

Methods and Results

We analyzed data from 746 Chinese American immigrants in the MESA study with a median follow‐up period of 17.8 years. The mean age of the cohort was 62.3 years, with 52.7% being women. Kaplan–Meier curves and Cox proportional hazards models were used to assess the association of immigration history, geographic location, biomarkers, and cardiac imaging parameters with cardiovascular risk factors and cardiovascular outcomes. The Cox hazards models were adjusted for known family history of heart disease, education level, sex, diabetes, hypertension, age, and body mass index. Although immigration history categorized as earlier (<20 years) versus later (≥20 years) showed no association with cardiovascular outcomes, the duration of residence in the United States emerged as a strong predictor for an increased risk of cardiovascular disease death (hazard ratio 1.39 [95% CI, 1.07–1.8];
P
=0.012). All‐cause mortality differed significantly between the Chinese immigrants from Los Angeles and those from Chicago, with higher survival probability in Chicago (log‐rank test,
P
=0.018). Furthermore, elevated levels of N‐terminal pro‐brain natriuretic peptide levels, left ventricular mass, and coronary artery calcium scores were associated with the risk of cardiovascular disease among Chinese immigrants.

Conclusions
Within the MESA cohort, the duration of residence and geographic location were associated with the risk of cardiovascular disease outcomes among Chinese immigrants.
Rural Hospital Performance in Guideline-Recommended Ischemic Stroke Thrombolysis, Secondary Prevention, and Outcomes
Authors: Man Shumei , Bruckman David , Uchino Ken , Chen Bing Yu , Dalton Jarrod E. , Fonarow Gregg C. 
Published: September 2024

BACKGROUND:
Existing data suggested a rural-urban disparity in thrombolytic utilization for ischemic stroke. Here, we examined the use of guideline-recommended stroke care and outcomes in rural hospitals to identify targets for improvement.

METHODS:
This retrospective cohort study included patients (aged ≥18 years) treated for acute ischemic stroke at Get With The Guidelines-Stroke hospitals from 2017 to 2019. Multivariable mixed-effect logistic regression was used to compare thrombolysis rates, speed of treatment, secondary stroke prevention metrics, and outcomes after adjusting for patient- and hospital-level characteristics and stroke severity.

RESULTS:
Among the 1 127 607 patients admitted to Get With The Guidelines-Stroke hospitals in 2017 to 2019, 692 839 patients met the inclusion criteria. Patients who presented within 4.5 hours were less likely to receive thrombolysis in rural stroke centers compared with urban stroke centers (31.7% versus 43.5%; adjusted odds ratio [aOR], 0.72 [95% CI, 0.68–0.76]) but exceeded rural nonstroke centers (22.1%; aOR, 1.26 [95% CI, 1.15–1.37]). Rural stroke centers were less likely than urban stroke centers to achieve door-to-needle times of ≤45 minutes (33% versus 44.7%; aOR, 0.86 [95% CI, 0.76–0.96]) but more likely than rural nonstroke centers (aOR, 1.24 [95% CI, 1.04–1.49]). For secondary stroke prevention metrics, rural stroke centers were comparable to urban stroke centers but exceeded rural nonstroke centers (aOR of 1.66, 1.94, 2.44, 1.5, and 1.72, for antithrombotics within 48 hours of admission, antithrombotics at discharge, anticoagulation for atrial fibrillation/flutter, statin treatment, and smoking cessation, respectively). In-hospital mortality was similar between rural and urban stroke centers (aOR, 1.11 [95% CI, 0.99–1.24]) or nonstroke centers (aOR, 1.00 [95% CI, 0.84–1.18]).

CONCLUSIONS:
Rural hospitals had lower thrombolysis utilization and slower treatment times than urban hospitals. Rural stroke centers provided comparable secondary stroke prevention treatment to urban stroke centers and exceeded rural nonstroke centers. These results reveal important opportunities and specific targets for rural health equity interventions.
Alexander of Alessandria’s Questions on Matter
Authors: De Pascalis Alessandro 
Published: September 2024

Abstract
The present article focuses on the questions on matter by the Franciscan Alexander of Alexandria (Lectura ii, d. 12). The article is divided into two main sections. The first section provides a philosophical-historical overview of Alexander’s questions on prime matter (qq. 1–2, 5). The second section provides insight into Alexander’s questions on heavenly matter (qq. 3–4), expounding the author’s solutions to the issues related to that subject. Appendix 1 contains a question list of Alexander’s unedited Lectura ii. An edition of Alexander’s Lectura ii, d. 12, qq. 1–5 is given in Appendix 2.
If You Live Right, Heaven Belongs to You
Authors: Millner Marlon 
Published: September 2024

Abstract
Early lectures, which became the book Prophesy Deliverance! by Cornel West, were delivered at the House of the Lord Pentecostal Church in Brooklyn, New York. The location of House of the Lord, which West argued was an exemplar of Afro-American revolutionary Christianity, demonstrates the relational and reciprocal insights Pentecostalism shares with West’s commitments to radical historicism and prophetic pragmatism. Several Black descended Pentecostals–scholars Leonard Lovett and Keri Day, and denominational leader Smallwood Williams–enact both a complex cultural analysis, and a robust social analysis. I argue Pentecostal praxis and critical reflection engage and extend West’s corpus and Black critical thought.
Is there a word from the Lord?
Authors: Delgado Dara Coleby 
Published: September 2024

Abstract
Black Apostolic Pentecostal Bishop Arthur M. Brazier employed various discursive practices to promote an integrationist agenda by affirming Black Power as a complex constellation of ideologies for achieving self-determination, Black pride, and self-sufficiency. Brazier deployed a Black Power/Black Liberation Theology-informed social program to help Black Chicagoans vie for their piece of the [American] pie in the name of cultural assimilation and socio-economic inclusion. Here, I reflect on Professor West’s proposal for an Afro-American Revolutionary Christianity. Recognizing that his discussion of the practical and programmatic dimensions of revolutionary Christian perspective and praxis were never intended to reify religious parochialism, in it, I find a theoretical framework with which to examine the religio-social consciousness of Black Pentecostals like Brazier, who prophesied deliverance via Black liberation.
Factors predicting employment status among persons with schizophrenia: A cross-sectional study from Chennai, India
Authors: Indhumathi T , Nisha B , Durairaj Jothilakshmi , Ramesh Kumar TC , Selva Savari Raj J , Swarup Adith , Ponnambalam Tejasvini , Raghavan Vijaya 
Published: September 2024

Background: Even among other mental disorders, a diagnosis of schizophrenia is associated with an abnormally low employment rate. However, those who can find employment report mental health improvements and diminishing symptoms. Aims: In this cross-sectional study, we analyzed a variety of sociodemographic factors between groups of schizophrenia-diagnosed employed and unemployed individuals to attempt to determine any causal relationships. Methods: A group of 52 employed and 48 unemployed individuals from the same outpatient hospital were surveyed. Patients were asked about their sociodemographic background and employment history, as well as subjected to a variety of tests to quantify critical aspects of their symptomatology. These included the Positive and Negative Syndrome Scale (PANSS), Social and Occupational Functioning Assessment Scale (SOFAS), and Personal and Social Performance Scale (PSP). The Calgary Depression Scale for Schizophrenia (CDSS) and Hamilton Anxiety Rating Scale (HAM-A) were administered to assess comorbid depression and anxiety. Lastly, the Simpson Angus Scale (SAS) measured any extrapyramidal side effects caused by the patients’ medications. Results: Initial analysis by the Student’s t-test and chi-square test revealed correlations between employment status and symptomatology, but upon performing logistic regression, peer support was found to be the only significant predictor of employment status (OR = 0.119, CI [0.40, 0.354], p = .001). Based on these results, the creation of peer support systems at work through fostering inclusive, well-informed, and destigmatized environments should be employers’ predominant focus. Conclusion: Future studies conducted longitudinally can strengthen the conclusions found and confirm the optimal manners in which to address the matter of aiding the integration of schizophrenic and similarly symptomatic individuals into the labor force.
Psychotropic medication and hepatobiliary health: Ultrasound observations on patients with schizophrenia
Authors: Yue Linlin , Sun Linlin , Li Nan 
Published: September 2024

Objective This study sought to examine the association between antipsychotic drug use and hepatobiliary health based on serum markers and ultrasound observations on a sample of patients with schizophrenia compared to age and gender matched healthy controls. Methods The 120 patients with schizophrenia and 60 control subjects had their blood drawn to measure liver function tests and underwent hepatobiliary ultrasonography to determine hepatobiliary lesions. Liver function tests included total cholesterol (TC), triglycerides (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Standardized cross-sectional images of the liver and kidneys were obtained from patients and controls, and analyses were stratified by length of taking psychotropic medication among those with schizophrenia. Liver echo attenuation coefficients, liver-kidney ratios, and liver fat content were determined. Results Psychotropic drug use was associated with greater liver burden and liver lesions in patients with schizophrenia compared to controls. The levels of TC, TG, ALT and AST in patients with schizophrenia were also all significantly higher among patients with schizophrenia. Long-term psychotropic medication was associated with increased levels of fatty liver in patients compared with controls. Levels of TC, TG, ALT and AST were all significantly higher in the long-term psychotropic medication use group than in the short-term group. Liver echo attenuation coefficient, liver-kidney ratio, and liver fat content were also higher in the long-term medication use group compared to the short-term group. Conclusion Antipsychotic drug use, particularly long-term use, is associated with increased liver burden in patients with schizophrenia, impaired lipid metabolism, increased liver lesions and fat content.
Optical coherence tomography angiography findings of systemic lupus erythematosus patients and the effect of neuropsychiatric involvement on it
Authors: Koyuncu Kevser , Ermurat Selime 
Published: September 2024

Aim To investigate the radial peripapillary capillary plexus vessel density (RPCP-VD) and peripapillary retinal nerve fiber layer thickness (pRNFLT) of systemic lupus erythematosus (SLE) and neuropsychiatric SLE patients (NPSLE) using disc optical coherence tomography angiography (OCTA) and investigate the association between these parameters and SLE disease activity index (SLEDAI-2K). Methods A total of 64 ′right eyes (36 SLE patients, 28 healthy controls (HCs)) were included in this cross-sectional case-control study. Ten (27.7%) patients had neuropsychiatric involvement. RPCP-VD and pRNFLT of patients were evaluated in all peripapillary sectors. RPCP-VD and pRNFLT of NPSLE, non-NPSLE, and HCs were compared. The correlation between SLEDAI-2K and OCTA findings was evaluated. Results SLE patients’ RPCP-VDs were significantly lower compared with the HCs except for two sectors ( p < .005). There was not a significant difference in pRNFLT of SLE patients and HCs. There was not a correlation between SLEDAI-2K and RPCP-VD in any subsectors but there was a significantly negative correlation between pRNFLT in tempo-inferior and inferior-temporal sectors. When compared with non-NPSLE-patients, NPSLE patients had significantly lower inferior-hemi ( p = .001), inferior-nasal VDs ( p = .003), and peripapillary ( p = .012), superior-hemi ( p = .038), inferior-hemi ( p = .026), inferior-nasal ( p = .002) and inferior-temporal ( p = .012) pRNFLTs. A negative correlation was found between NPSLE and pRNFLT. Conclusion SLE patients may have early subclinical vascular involvement leading to decreased RPCP-VD. A negative correlation between the SLEDAI-2K and pRNFLT in the temporal subsectors of all SLE patients may show an association between the disease activity and temporal pRNFL thinning. The presence of neuropsychiatric involvement may also be associated with decreased RPCP-VD and pRNFLT.
Cortexa: a comprehensive resource for studying gene expression and alternative splicing in the murine brain
Authors: Weißbach Stephan , Milkovits Jonas , Pastore Stefan , Heine Martin , Gerber Susanne , Todorov Hristo 
Published: September 2024

Abstract
Background
Gene expression and alternative splicing are strictly regulated processes that shape brain development and determine the cellular identity of differentiated neural cell populations. Despite the availability of multiple valuable datasets, many functional implications, especially those related to alternative splicing, remain poorly understood. Moreover, neuroscientists working primarily experimentally often lack the bioinformatics expertise required to process alternative splicing data and produce meaningful and interpretable results. Notably, re-analyzing publicly available datasets and integrating them with in-house data can provide substantial novel insights. However, such analyses necessitate developing harmonized data handling and processing pipelines which in turn require considerable computational resources and in-depth bioinformatics expertise.

Results
Here, we present Cortexa—a comprehensive web portal that incorporates RNA-sequencing datasets from the mouse cerebral cortex (longitudinal or cell-specific) and the hippocampus. Cortexa facilitates understandable visualization of the expression and alternative splicing patterns of individual genes. Our platform provides SplicePCA—a tool that allows users to integrate their alternative splicing dataset and compare it to cell-specific or developmental neocortical splicing patterns. All standardized gene expression and alternative splicing datasets can be downloaded for further in-depth downstream analysis without the need for extensive preprocessing.

Conclusions
Cortexa provides a robust and readily available resource for unraveling the complexity of gene expression and alternative splicing regulatory processes in the mouse brain. The data portal is available at https://cortexa-rna.com/
A putative scenario of how de novo protein-coding genes originate in the Saccharomyces cerevisiae lineage
Authors: Yada Tetsushi , Taniguchi Takeaki 
Published: September 2024

Abstract
Background
Novel protein-coding genes were considered to be born by re-organization of pre-existing genes, such as gene duplication and gene fusion. However, recent progress of genome research revealed that more protein-coding genes than expected were born de novo, that is, gene origination by accumulating mutations in non-genic DNA sequences. Nonetheless, the in-depth process (scenario) for de novo origination is not well understood.

Results
We have conceived bioinformatic analysis for sketching a scenario for de novo origination of protein-coding genes. For each de novo protein-coding gene, we firstly identified an edge of a given phylogenetic tree where the gene was born based on parsimony. Then, from a multiple sequence alignment of the de novo gene and its orthologous regions, we constructed ancestral DNA sequences of the gene corresponding to both end nodes of the edge. We finally revealed statistical features observed in evolution between the two ancestral sequences. In the analysis of the Saccharomyces cerevisiae lineage, we have successfully sketched a putative scenario for de novo origination of protein-coding genes. (1) In the beginning was GC-rich genome regions. (2) Neutral mutations were accumulated in the regions. (3) ORFs were extended/combined, and then (4) translation signature (Kozak consensus sequence) was recruited. Interestingly, as the scenario progresses from (2) to (4), the specificity of mutations increases.

Conclusion
To the best of our knowledge, this is the first report outlining a scenario of de novo origination of protein-coding genes. Our bioinformatic analysis can capture events that occur during a short evolutionary time by directly observing the evolution of the ancestral sequences from non-genic to genic. This property is suitable for the analysis of fast evolving de novo genes.