bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stores Licensing Scheme in remote Indigenous communities of the Northern Territory, Australia: a meta-evaluation
Authors: Howes Sophie K. , van Burgel Emma , Cubillo Beau , Connally Sarah , Ferguson Megan , Brimblecombe Julie 
Published: September 2024

AbstractA form of food retail regulation called the Stores Licensing Scheme was introduced by the Australian Government in 2007–2022 to ensure food security in remote Indigenous communities of the Northern Territory. We examined evaluations of this Scheme implemented under the Northern Territory National Emergency Response and Stronger Futures Northern Territory Acts. Grey literature search identified nine primary source evaluations. Reported outcomes were extracted and thematic analysis utilised to determine barriers and enablers. Outcomes included improved availability and quality of groceries, financial structures, and retail practices, albeit not consistently reported. Governance and food cost were perceived barriers. Future policy aimed to improve food security through community stores should consider food cost subsidy, measures to incentivise all stores to improve standards, and improved governance arrangements enabling self-determination for Aboriginal and Torres Strait Islander Store Directors.
Extracellular vesicles from highly invasive melanoma subpopulations increase the invasive capacity of less invasive melanoma cells through mir-1246-mediated inhibition of CCNG2
Authors: Kingreen Tim , Kewitz-Hempel Stefanie , Rohde Christian , Hause Gerd , Sunderkötter Cord , Gerloff Dennis 
Published: September 2024

AbstractInvasive growth is a critical process in tumor progression, requiring the activation of various molecular processes in tumor cells at the invasive front. Intercellular communication between heterogeneous tumor cells enhances cellular activation and adaptation to specific microenvironments. One mechanism of intercellular communication is the delivery of miRNAs through tumor cell-derived extracellular vesicles (EVs). In this context we have observed that conditioned media from a highly invasive cell subpopulation (BLM-HI) enhances the invasive capacity of the parental cell line (BLM). Therefore, we hypothesized that this complex change of cellular behavior is influenced by EV-transported miRNAs. The treatment of BLM cells with EVs derived from BLM-HI cells resulted in a significantly enhanced invasive capacity, as observed in Matrigel-embedded spheroids and in 2D Boyden chamber assays, with a dose-dependent effect. Conversely, the invasive capacity of BLM cells was reduced when secretion of EVs was inhibited by a sphingomyelinase inhibitor. To investigate the molecular mechanisms behind this effect, we performed next-generation sequencing and identified an enrichment of miR-1246 in these EVs. In functional analyses we demonstrated that both the EV mediated delivery of miR-1246 as well as overexpression contributes to the enhanced invasiveness of BLM cells. We identified a binding site of miR-1246 in the 3’UTR of cyclin G2 (CCNG2) and demonstrated direct binding by a luciferase reporter assay.Increased expression of CCNG2 has been associated with cancer metastasis and poor patient outcomes in other malignancies. Our study demonstrates that intercellular communication contributes to the transfer of properties, such as increased invasive capacity, between heterogeneous melanoma cells via EV-transported miRNAs.
Rapid UV-Vis spectrophotometric method aided by firefly-PLS models for the simultaneous quantification of ciprofloxacin, lomefloxacin, and enrofloxacin in their laboratory mixture, dosage forms and water samples: greenness and blueness assessment
Authors: Alqahtani Ali , Alqahtani Taha , Al Fatease Adel , Tolba Enas H. 
Published: September 2024

AbstractHerein, a novel UV spectrophotometric method coupled with chemometric tools was developed for the simultaneous determination of three fluoroquinolone antibiotics: ciprofloxacin, lomefloxacin, and enrofloxacin. Such integration of UV spectroscopy and chemometric analysis proved to be a simple, rapid, and cost-effective approach for the quantification of these clinically important pharmaceutical compounds and aid in their quality control analysis. The method employed firefly algorithm for variable selection and partial least squares (PLS) regression for model calibration. The developed method was validated by independent test set in addition the accuracy, intra and inter-day precision as per ICH guidelines which showed a satisfactory performance with mean recovery ranged between 98.18 and 101.83 with %RSD < 2. Besides, the developed method displayed ultrasensitive levels with LODs (0.0803, 0.1125, 0.1309 µg/mL) and LOQs (0.2434, 0.3409, 0.3968 µg/mL) for ciprofloxacin, lomefloxacin, and enrofloxacin, respectively. The greenness and blueness of the developed method were also evaluated using the recently proposed Analytical GREEnness metric approach (AGREE) and Blue applicability grade index (BAGI) tools, which showed a high AGREE score of 0.79 and a BAGI score of 77.5. These results indicate that the developed method provides an environmentally friendly alternative to the traditionally used chromatographic techniques, while maintaining high analytical practicability. Finally, the application of the developed methodology was demonstrated on real pharmaceutical and tap water samples, and the results were in good agreement with those obtained by the reference HPLC method indicating the reliability and suitability of the proposed spectrophotometric method for routine analysis of fluoroquinolone antibiotics.
Highlight selection of radiochemistry and radiopharmacy developments by editorial board
Authors: Scott Peter JH. , Penuelas Ivan , Rey Ana , Aime Silvio , Ambikalmajan Pillai M.R. , Antunes Ines Farinha , Cleeren Frederik , Liu Zhaofei , Ellis Beverley , Kahts Maryke , Ekoume Fany Pricile , Chaple Ivis F. , Bernardes Emerson , Behe Martin , Huang Ya-Yao , Mikolajczak Renata , Furumoto Shozo , Elrefaei Amal , Kopka Klaus 
Published: September 2024

Abstract
Background
The Editorial Board of EJNMMI Radiopharmacy and Chemistry releases a biannual highlight commentary to update the readership on trends in the field of radiopharmaceutical development.

Main body
This selection of highlights provides commentary on 19 different topics selected by each coauthoring Editorial Board member addressing a variety of aspects ranging from novel radiochemistry to first-in-human application of novel radiopharmaceuticals.

Conclusion
Trends in radiochemistry and radiopharmacy are highlighted. Hot topics cover the entire scope of EJNMMI Radiopharmacy and Chemistry, demonstrating the progress in the research field in many aspects.
Physiological responses of Lizard, Toad and Pigeon during rainy season in tropical savanna climate
Authors: Ajayi Ayodeji Folorunsho , Ogundipe Oluwadare Joshua , Ajayi Lukman Olawale , Oyerinde Abiodun , Akinpelu Omolola Funke 
Published: September 2024

Abstract
Background
This study investigated the adaptation mechanisms of Pigeons, Toads and Lizards during the rainy season in tropical savanna climates. Male Agama Lizards (Agama agama), Afep Pigeons (Columba unicincta) and Toads (Sclerophrys perreti) were captured at a location in Ibadan, Oyo state. Five (5) Lizards marked: L1 to L5, Afep Pigeons marked: B1 to B5 and Toads marked: T1 to T5. Each of the animals was manually constrained within six to eight hours of capture, and a 1.0-ml syringe was used to obtain blood from the heart or coccygeal vein. Blood samples were collected in an EDTA and plain bottle, respectively. Plasma and sera samples were separated by centrifugation at − 4 °C using a cold centrifuge and then analysed for creatinine, urea, glucose, Mg+, k+, Cl−, Na+, albumin, TP, ALP, ALT, AST, GGT, MDA, SOD, CAT, GSH and haematological parameters. Cervical dislocation was performed on the animals before organs such as kidneys and liver were collected from each of the animals. Each organ collected from each animal was placed in separate plain tubes (filled with phosphate buffer).

Result
The Pigeon had higher body temperature, urea, glucose, ALP, PCV, haemoglobin concentration, neutrophil and triglyceride compared to the Lizard and Toad, while the Toad had higher Na+, Mg+ and Cl−, basophil and monocytes compared to Pigeon and Lizard and the Lizard has higher creatinine, lymphocyte and cholesterol compared to the Pigeon and Toad. On the liver oxidative stress markers, the Pigeon has higher superoxide dismutase and reduced glutathione compared to the Lizard and Toad, while the Toad has higher MDA and catalase compared to the Pigeon and Lizard. On the kidney oxidative stress markers, the Pigeon has higher MDA compared to the Lizard and Toad, while the Toad has higher catalase than the Pigeon and Lizard.

Conclusions
From this study, the elevated level of lymphocytes in Lizard and eosinophil, basophil and monocytes in Toad suggests that Lizards and Toad are more vulnerable to inflammation. The high value of cholesterol in Lizard and triglyceride in Pigeon as observed in this study may relate to the degree of stress. Also, the activation of antioxidant systems under comparative study is a part of the survival strategy of animals like amphibians, reptiles and aves when facing environmental problems.
Role of pleural manometry and transthoracic ultrasonography to predict entrapped lung
Authors: Youssef Shaimaa Magdy Abou , Mohamed Ahmed Abdelsadek , Amin Diaa Eldin Abdelazeem , Amin Nashwa Ahmed 
Published: September 2024

Abstract
Introduction
Unexpandable lung is a complication by which the lung does not expand to the chest wall with pleural space drainage, which will result in adverse events or intervention failure if not well recognized prior to the intervention.

Aim of the work
This study aimed to assess the role of pleural manometry and transthoracic ultrasonography in predicting abnormal lung expansion during pleural drainage.

Patients and methods
This was a prospective observational analytical study involving 50 patients that aims to predict abnormal lung expansion during pleural drainage using pleural manometry and transthoracic ultrasound.

Results
Regarding pleural manometry (pleural elastance), all patients in the entrapped lung group (100%) had pleural elastance > 14.5 cmH2o/L, while in the non-entrapped lung group, all patients (100%) had pleural elastance ≤ 14.5 cmH2o/L. Regarding ultrasonography, pleural thickening > 0.5 cm was found in all patients with entrapped lung (100%) and 50% of patients with non-entrapped lung with statistically significant difference. In the entrapped lung group, 20%, 35%, and 45% of patients had simple, complex non-septated, and complex septated pleural effusion, respectively, while in the non-entrapped lung group, 63.35%, 33.33%, and 3.32% of patients had simple, complex non-septated, and complex septated effusion, respectively, with statistically significant difference (p-value 0.0005).

Conclusion
Pleural manometry and transthoracic ultrasound can guide decision-making regarding the timing of pleural interventions and management of cases with entrapped lung.
Fasting in critical illness: the role of ketonuria — a retrospective observational study
Authors: Ottaviani Irene , Tantillo Simona , Miggiano Lorenzo , Guarnera Martina , Menghini Marco , Talarico Francesco , Mazzanti Federica , Cilloni Nicola 
Published: September 2024

Abstract
Background
Metabolic acidosis is a frequent finding in patients admitted to the intensive care unit (ICU). It can be caused by prolonged fasting due to surgical procedures or by medical conditions that lead to starvation ketoacidosis (SKA). Early recognition and treatment of SKA could prevent several life-threatening complications, improving survival and reducing the ICU length of stay.

Methods
We retrospectively screened all medical records of patients admitted to the ICU (Maggiore Hospital, Bologna, North Italy) from May 2022 to April 2023. We included patients aged 18 years or older who presented ketonuria detected in the urine sample.

Results
We analyzed 190 patients with ketonuria at ICU admission. Postsurgical patients showed lower levels of albumin and a higher rate of shock compared to medical patients. Ketonuric patients with shock had a lower body mass index (BMI) compared to patients without shock (24 versus 26 kg/m2, respectively). There were no differences within groups regarding mortality and ICU readmission rate. Medical patients had a significantly higher ICU length of stay.

Conclusions
This retrospective observational descriptive study showed that patients with ketonuria, hypoalbuminemia, and low BMI at ICU admission have high risk of hemodynamic instability and shock. Surgical patients compared to medical patients are exposed to a catabolic trigger that could worsen a state of malnutrition and induce anabolic resistance; elective and urgent surgical patients did not differ in terms of risk of shock and mortality, probably due to the activation of this catabolic pathway. Early recognition and treatment of starvation ketoacidosis and perioperative nutritional optimization could reduce incidence of hemodynamic and metabolic complications.
Cryoablation Without Excision for Early-Stage Breast Cancer: ICE3 Trial 5-Year Follow-Up on Ipsilateral Breast Tumor Recurrence
Authors: Fine Richard E. , Gilmore Richard C. , Tomkovich Kenneth R. , Dietz Jill R. , Berry Michael P. , Hernandez Lydia E. , Columbus Karen S. , Seedman Susan A. , Fisher Carla S. , Han Linda K. , Manahan Eric R. , Hicks Randy D. , Vaidya Rashmi P. , Curcio Lisa D. , Sevrukov Alexander B. , Kenler Andrew S. , Taback Bret , Chen Margaret , Miller Megan E. , Gold Linsey , Anglin Beth V. , Aoun Hussein D. , Simmons Rache M. , Feldman Sheldon M. , Boolbol Susan K. 
Published: September 2024

Abstract
Background
The ICE3 trial evaluated the safety and efficacy of cryoablation in women aged ≥60 years with low-risk, early-stage breast cancers, aiming to provide a non-operative treatment option and avoid potential surgical risks. This study presents 5-year follow-up trial results.

Methods
The ICE3 trial is an Institutional Review Board-approved, prospective, multicentered, non-randomized trial including women ≥ 60 years of age with unifocal, ultrasound visible, invasive ductal carcinoma ≤ 1.5 cm in size, histologic grade 1–2, hormone receptor (HR)-positive, and human epidermal growth factor receptor 2 (HER2)-negative. The primary study endpoint of 5-year ipsilateral breast tumor recurrence (IBTR) was evaluated based on Kaplan–Meier estimates.

Results
Overall, 194 patients meeting eligibility received successful cryoablation treatment per protocol and were included for analysis. The mean age was 74.9 years (55–94) with a mean tumor size of 7.4 mm transverse (2.8–14.0 mm) and 8.1 mm sagittal (2.5–14.9 mm). With a mean follow-up period of 54.16 months, the IBTR rate at 5 years was 4.3% and breast cancer survival was 96.7%. Of the 124 patients who received endocrine therapy only, the IBTR was 3.7%. No serious device-related adverse events were reported. Minor (88.2%) and moderate (9.6%) adverse events were mild in severity and resolved without residual effects. Quality-of-life score demonstrated statistically significant improvement (p < 0.001) in distress at 6 months as compared with baseline.

Conclusions
Breast cryoablation presents a promising alternative to surgery in selected patients, offering the benefits of a minimally invasive procedure with minimal risks. Further studies are encouraged to confirm cryoablation as a viable alternative to surgical excision low-risk patients.
Effects of Sports Bras and Breast Volume on Pulmonary System and Respiratory Symptom Responses to Exercise in Healthy Females
Authors: ILLIDI CAMILLA R. , JENSEN DENNIS 
Published: September 2024

ABSTRACT

Purpose
This study assessed the individual and combined effects of sports bras and breast volume on pulmonary system and respiratory symptom responses to exercise in recreationally active females.

Methods
Twenty-three healthy females (18–27 yr; bra sizes 32B–36DDD) were divided into small and large breast volume groups (SBV and LBV, respectively) around median volume (324 mL; mean ± SD SBV: 284 ± 38 mL vs LBV: 560 ± 97 mL; P < 0.001, g = 3.84). Participants completed three 5-min bouts of constant-load cycle exercise at 30%, 60%, and 90% of their peak power output while wearing a high-support sports bra, low-support sports bralette, or their personal (self-selected) sports bra in randomized order. Measurements included ventilation, breathing pattern, respiratory pressures, diaphragm electromyogram (EMGdi), and ratings of perceived exertion (RPE), breathlessness (intensity and unpleasantness), and chest tightness due to bra.

Results
Compared with low-support and personal sports bras, the high-support sports bra evoked stronger sensations of restricted breathing and chest tightness (“slight” to “moderate” restriction in n = 7 (30%) vs n = 1 (4%) in low-support and personal bras; P = 0.014). There was, however, no evidence of greater concomitant inspiratory constraints, EMGdi, or inspiratory muscle pressure generation in either bra (all P > 0.05). Notably, LBV compared with SBV, participants reported greater RPE (P = 0.037, η
p
2 = 0.20), breathlessness intensity (P = 0.039, η
p
2 = 0.20), and unpleasantness (P = 0.041; η
p
2 = 0.19), which, in the setting of comparable pulmonary system responses to exercise, was likely driven by stronger perceived chest tightness and/or bra awareness in LBV participants.

Conclusions
Despite evoking mild-to-moderately severe chest tightness due to bra during exercise, correctly fitted sports bras, whether low- or high-support, do not impose a physiological burden to the respiratory system and its response to mild-to-heavy intensity exercise in otherwise healthy females.
Σχεδιασμός και Πιλοτική Εφαρμογή Διδακτικής Μαθησιακής Ακολουθίας για την Εννοιολογική Κατανόηση της Eντροπίας από Υποψήφιους Εκπαιδευτικούς μέσω μιας Μικροσκοπικής Προσέγγισης
Authors: Μπακάλη Βάια , Ασημόπουλος Στέφανος 
Published: September 2024

Η παρούσα μελέτη πραγματεύεται την ανάπτυξη μιας Διδακτικής Μαθησιακής Ακολουθίας για την εννοιολογική κατανόηση της εντροπίας και των εννοιών που εμπλέκονται με αυτή σε μια μικροσκοπική προσέγγιση. Χρησιμοποιώντας ως πλαίσιο το Μοντέλο της Διδακτικής Αναδόμησης για τη συλλογή των δεδομένων εφαρμόστηκε το διδακτικό πείραμα σε ζευγάρια φοιτητριών Παιδαγωγικού τμήματος. Μέσω των αναλύσεων των πορειών μάθησης των φοιτητριών επιδιώκεται η ανάδειξη των σχέσεων που δημιουργούνται μεταξύ των κυρίαρχων εννοιών στη ΔΜΑ και η αποτύπωση του ρόλου των μοντέλων που εφαρμόστηκαν για τη μικροσκοπική προσέγγιση της εντροπίας. Καταγράφονται οι εξελισσόμενες αντιλήψεις των φοιτητριών αναφορικά με τις έννοιες, οι δυσκολίες που αντιμετώπισαν και ο τρόπος συσχέτισής τους με τα εν λόγω μοντέλα.
Σχεδιασμός και ανάπτυξη μεθοδολογίας για την κατασκευή εφαρμογών Προσαρμοστικής Παιχνιδοποίησης στις Φ.Ε
Authors: Ζουρμπάκης Αλκίνοος Ιωάννης , Καλογιαννάκης Μιχαήλ 
Published: September 2024

Η εκπαίδευση στις Φ.Ε είναι απαραίτητη για την απόκτηση επιστημονικού γραμματισμού. Όμως, οι επιστημονικές έννοιες είναι συχνά δυσκολονόητες και μπορεί να οδηγήσουν τους μαθητές στην ανάπτυξη εναλλακτικών αντιλήψεων. Μία ταχεία αναπτυσσόμενη τάση στην ενσωμάτωση νέων τεχνολογιών στην εκπαίδευση είναι η προσαρμοστική παιχνιδοποίηση. Οι τεχνολογικές εφαρμογές που χρησιμοποιούνται στην εκπαίδευση θα πρέπει να σχεδιάζονται σε συσχέτιση με το μαθησιακό περιεχόμενο, παιδαγωγικές προσεγγίσεις, στρατηγικές μάθησης και χαρακτηριστικά για την κατασκευή της γνώσης των μαθητών. Η παρούσα μελέτη έχει ως σκοπό την ανάπτυξη μιας μεθοδολογίας προσαρμοστικής παιχνιδοποίησης που θα βασίζεται συσχετιζόμενες θεωρίες ψυχολογίας και κινήτρων, ενσωματώνοντας προσαρμοστικά κριτήρια, στρατηγικές μάθησης, στοιχεία παιχνιδιού και όλες τις ζωτικές πτυχές της μαθησιακής διαδικασίας που σχετίζονται με την εκπαίδευση στις Φ.Ε.
Use of three-dimensional acellular collagen matrix in deep or tunnelling diabetic foot ulcers: a retrospective case series
Authors: Abdo Raymond J , Couch Amy L 
Published: September 2024

Objective: While most xenograft wound matrices are flat sheets not designed for deep or tunnelling wounds, three-dimensional acellular collagen matrices (3D-ACM) can fill deep wound beds and enable full wound wall apposition. This case series examines the use of 3D-ACM in treating diabetic foot ulcers (DFUs) that are deep, tunnelling, undermining, or irregularly shaped. We report outcomes of cases where 3D-ACM was applied to deep or tunnelling DFUs present for at least four weeks. Method: In this retrospective case series, 3D-ACM was applied, healing was monitored and measurements were collected. Additional 3D-ACM was applied as needed. Results: In total, 11 patients with 13 wounds were treated. Improved wound appearance and reduced size were observed at most follow-ups. Mean initial wound depth was 1.6cm, and several wounds showed significant depth reductions shortly after therapy initiation. In total, 62% of wounds (8/13) reached 50% closure by four weeks. Additionally, 54% (7/13) were fully closed by 12 weeks. The remaining 46% (6/13) took between 12–22.3 weeks to heal. Overall mean therapy time was 13.1 weeks (range: 2.0–22.3 weeks). Deeper wounds generally took longer to close. Conclusion: The findings of this case series showed that 3D-ACM could offer a protective microenvironment for wound management for deep or tunnelling DFUs. While some took >12 weeks to close, this may be attributable to large initial depths and volumes, rather than a failure to respond to the treatment modality. Other wounds that require a conforming 3D matrix, enabling full wound wall apposition, may benefit from 3D-ACM. Further investigations would be beneficial to understand the capabilities of this treatment modality.