bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Molecular Activity Painting”: Switch‐like, Light‐Controlled Perturbations inside Living Cells
Authors: Chen Xi , Venkatachalapathy Muthukumaran , Kamps Dominic , Weigel Simone , Kumar Ravi , Orlich Michael , Garrecht Ruben , Hirtz Michael , Niemeyer Christof M. , Wu Yao‐Wen , Dehmelt Leif 
Published: March 2017

AbstractAcute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present “molecular activity painting” (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface‐immobilized antibodies blocks lateral diffusion, enabling rapid and stable “painting” of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA‐myosin activator GEF‐H1 induces patterned acto‐myosin contraction inside living cells.
Substrate‐Assisted, Transition‐Metal‐Free Diboration of Alkynamides with Mixed Diboron: Regio‐ and Stereoselective Access to trans‐1,2‐Vinyldiboronates
Authors: Verma Astha , Snead Russell F. , Dai Yumin , Slebodnick Carla , Yang Yinuo , Yu Haizhu , Yao Fu , Santos Webster L. 
Published: March 2017

AbstractA substrate‐assisted diboration of alkynamides using the unsymmetrical pinacolato‐1,8‐diaminonaphthalenato diboron (pinBBdan) is described. The transition‐metal‐free reaction proceeds in a regio‐ and stereoselective fashion to exclusively afford trans‐vinyldiboronates in good to excellent yields. Notably, Bdan and Bpin are installed on the α‐ and β‐carbon atoms, respectively.
Template‐Directed Approach Towards the Realization of Ordered Heterogeneity in Bimetallic Metal–Organic Frameworks
Authors: Kim Daeok , Coskun Ali 
Published: March 2017

AbstractControlling the arrangement of different metal ions to achieve ordered heterogeneity in metal–organic frameworks (MOFs) has been a great challenge. Herein, we introduce a template‐directed approach, in which a 1D metal–organic polymer incorporating well‐defined binding pockets for the secondary metal ions used as a structural template and starting material for the preparation of well‐ordered bimetallic MOF‐74s under heterogeneous‐phase hydrothermal reaction conditions in the presence of secondary metal ions such as Ni2+ and Mg2+ in 3 h. The resulting bimetallic MOF‐74s were found to possess a nearly 1:1 metal ratio regardless of their initial stoichiometry in the reaction mixture, thus demonstrating the possibility of controlling the arrangement of metal ions within the secondary building blocks in MOFs to tune their intrinsic properties such as gas affinity.
Attitude Tracking Control of A Quad‐Rotor with Partial Loss of Rotation Effectiveness
Authors: Song Zhankui , Sun Kaibiao 
Published: March 2017

AbstractThis paper investigates the attitude control problem of a quad‐rotor unmanned helicopter. In response to adverse factors, including the lumped disturbance, inertia parameter uncertainties, and the partial loss of rotation effectiveness, an adaptive compensation control strategy combining the terminal sliding mode technique and the input shaping method is proposed. Specifically, a group of updating laws using an adaptive mechanism is added to adjust the control strategy in a manner conductive to attitude stability and performance preservation in the presence of adverse factors. The key features of the proposed control strategy are that it is independent from the knowledge of actuator faults, and adaptive compensation control is achieved without the need for online identification of rotor failure. The finite time convergence and stability of the attitude tracking errors are proved by using Lyaponov's method. Finally, the simulation results demonstrate the effectiveness of the proposed control strategy.
Determination of fluvoxamine maleate in human urine and human serum using alkaline KMnO4–rhodamine B chemiluminescence
Authors: Yang Dongqin , He Yanyan , Chen Funan 
Published: March 2017

AbstractThe flow‐injection chemiluminescence (FI‐CL) behavior of a gold nanocluster (Au NC)–enhanced rhodamine B–KMnO4 system was studied under alkaline conditions for the first time. In the present study, the as‐prepared bovine serum albumin‐stabilized Au NCs showed excellent stability and reproducibility. The addition of trace levels of fluvoxamine maleate (Flu) led to an obvious decline in CL intensity in the rhodamine B–KMnO4–Au NCs system, which could be used for quantitative detection of Flu. Under optimized conditions, the proposed CL system exhibited a favorable analytical performance for Flu determination in the range 2 to 100 μg ml−1. The detection limit for Flu measurement was 0.021 μg ml−1. Moreover, this newly developed system revealed outstanding selectivity for Flu detection when compared with a multitude of other species, such as the usual ions, uric acid and a section of hydroxy compounds. Additionally, CL spectra, UV–visible spectroscopes and fluorescence spectra were measured in order to determine the possible reaction mechanism. This approach could be used to detect Flu in human urine and human serum samples with the desired recoveries and could have promising application under physiological conditions.
Avian embryos and related cell lines: A convenient platform for recombinant proteins and vaccine production
Authors: Farzaneh Maryam , Hassani Seyedeh‐Nafiseh , Mozdziak Paul , Baharvand Hossein 
Published: March 2017

AbstractChick embryos are a significant historical research model in basic and applied sciences. The embryonated eggs have been used for virus inoculation in order to vaccine production for nearly a century. Recently, avian eggs and cell lines derived from embryonated eggs have found wide application in biotechnology. This review will discuss about the unique characteristics of avian eggs in terms of safety, large scale and economical production of recombinant proteins. This system also provides the human‐like glycosylation on target proteins and therefore can be considered as a suitable host for biomanufacturing of humanized monoclonal antibodies and therapeutic proteins. Avian derived cell lines are an alternative for rapid vaccine manufacturing during a pandemic. Based on the latest knowledge in cell and animal transgenesis, the currently available germ cell‐mediated gene transfer system provides a more efficient strategy in gene targeting and creation of transgenic birds that lead to advancements in industrial, biotechnology, and biological research applications. This review covers the recent development of avian fertilized eggs and related cell lines in a variety of human biopharmaceuticals and viral vaccine manufacturing.
MicroRNA: an important regulator in acute myeloid leukemia
Authors: Wang Xiaman , Chen Hongli , Bai Ju , He Aili 
Published: March 2017

AbstractMicroRNAs (miRNAs) are a general class of endogenous non‐coding RNAs with a length of 22 nucleotides, widely existing in diverse species and playing important roles in malignancies initiation and progression. MiRNAs are essential to many in vivo biological processes such as cell proliferation, apoptosis, immune response, and tumorigenesis. Significant progress till date has been made in understanding the roles of microRNAs in normal hematopoiesis and hematopoietic malignant diseases. In this review, we summarize the particular signatures of microRNAs in acute myeloid leukemia (AML) patients with specific karyotype and the clinical significance of microRNAs in early diagnosis and treatment. MicroRNAs hypermethylation was also proved to correlate with the pathogenesis of AML. However, the target genes and exact pathways of microRNAs participating in these processes are still unknown and more efforts need to be made in the near future.
An alternative technique to safely close the chest after placing a child on central venoarterial extracorporeal membrane oxygenation
Authors: Bobos Dimitrios , Kanakis Meletios A. , Koulouri Sofia , Rammos Spyridon , Karabinis Andreas , Giannopoulos Nicholas M. 
Published: March 2017

Key Clinical MessageCentral venoarterial (VA) placement of extracorporeal membrane oxygenation (ECMO) is performed surgically, and in the majority of cases, the patient remains with an open sternum. Herein, a case of a 3‐year‐old patient who underwent insertion of a central VA ECMO for heart failure due to acute myocarditis is described. An alternative technique for ECMO placement providing sternal closure and minimizing infection risk for the safe patient transport is described.
Formation of a Cubic Liquid Crystalline Nanostructure with π‐Conjugated Fluorinated Rods on the Gyroid Minimal Surface
Authors: Poppe Marco , Chen Changlong , Liu Feng , Poppe Silvio , Tschierske Carsten 
Published: March 2017

AbstractBicontinuous cubic phases are of significant importance for numerous applications, for example, for the crystallization of membrane proteins, as photonic materials and as templates for porous silica. A new variant of bicontinuous cubic liquid crystalline phase with Ia
d lattice is reported herein for X‐shaped bolapolyphiles. It was shown that in this class of compounds, cubic‐phase induction can be achieved by proper aromatic core fluorination; in addition, the first cubic phases having π‐conjugated oligo(phenylene ethynylene) rods on the gyroid minimal surface were obtained. These new structures composed of 3D folded layers of parallel arranged π‐conjugated rods should allow charge transport in all three dimensions, which is of interest for organic semiconductor applications.
Homochiral Self‐Sorted and Emissive IrIII Metallo‐Cryptophanes
Authors: Pritchard Victoria E. , Rota Martir Diego , Oldknow Samuel , Kai Shumpei , Hiraoka Shuichi , Cookson Nikki J. , Zysman‐Colman Eli , Hardie Michaele J. 
Published: March 2017

AbstractThe racemic ligands (±)‐tris(isonicotinoyl)‐cyclotriguaiacylene (L1), or (±)‐tris(4‐pyridyl‐methyl)‐cyclotriguaiacylene (L2) assemble with racemic (Λ,Δ)‐[Ir(ppy)2(MeCN)2]+, in which ppy=2‐phenylpyridinato, to form [{Ir(ppy)2}3(L)2]3+ metallo‐cryptophane cages. The crystal structure of [{Ir(ppy)2}3(L1)2]⋅3BF4 has MM‐ΛΛΛ and PP‐ΔΔΔ isomers, and homochiral self‐sorting occurs in solution, a process accelerated by a chiral guest. Self‐recognition between L1 and L2 within cages does not occur, and cages show very slow ligand exchange. Both cages are phosphorescent, with [{Ir(ppy)2}3(L2)2]3+ having enhanced and blue‐shifted emission when compared with [{Ir(ppy)2}3(L1)2]3+.
Chromatographic enantioseparation by poly(biphenylylacetylene) derivatives with memory of both axial chirality and macromolecular helicity
Authors: Ishidate Ryoma , Ikai Tomoyuki , Kanoh Shigeyoshi , Yashima Eiji , Maeda Katsuhiro 
Published: March 2017

AbstractNovel poly(biphenylylacetylene) derivatives bearing two acetyloxy groups at the 2‐ and 2′‐positions and an alkoxycarbonyl group at the 4′‐position of the biphenyl pendants (poly‐Ac's) were synthesized by the polymerization of the corresponding biphenylylacetylenes using a rhodium catalyst. The obtained stereoregular (cis‐transoidal) poly‐Ac's folded into a predominantly one‐handed helical conformation accompanied by a preferred‐handed axially twisted conformation of the biphenyl pendants through noncovalent interactions with a chiral alcohol and both the induced main‐chain helicity and the pendant axial chirality were maintained, that is, memorized, after complete removal of the chiral alcohol. The stability of the helicity memory of the poly‐Ac's in a solution was lower than that of the analogous poly(biphenylylacetylene)s bearing two methoxymethoxy groups at the 2‐ and 2′‐positions of the biphenyl pendants (poly‐MOM's). In the solid state, however, the helicity memory of the poly‐Ac's was much more stable and showed a better chiral recognition ability toward several racemates than that of the previously reported poly‐MOM when used as a chiral stationary phase for high‐performance liquid chromatography. In particular, the poly‐Ac‐based CSP with a helicity memory efficiently separated racemic benzoin derivatives into enantiomers.
A Comparative Pharmacokinetic Study of 2 Pemetrexed Formulations in Indian Adult Chemonaive Patients With Adenocarcinoma Stage III/IV Non–Small Cell Lung Cancer
Authors: Kavathiya Krunal , Gurjar Murari , Patil Anand , Naik Madhura , Noronha Vanita , Joshi Amit , Gota Vikram , Prabhash Kumar 
Published: March 2017

AbstractThe study aimed to compare the pharmacokinetics of 2 pemetrexed formulations (Pemgem, Dr. Reddy's Laboratories w.r.t; Alimta, Eli Lilly) in adult chemonaive subjects with adenocarcinoma stage III/IV non–small cell lung cancer. All patients received 500 mg/m2 pemetrexed (Alimta or Pemgem) as a 10‐minute infusion on day 1 of a 21‐day cycle. Plasma pemetrexed concentrations were determined on day 1 of cycle 1. Area under the concentration–time curve (AUC0–inf) and the maximum plasma concentration (Cmax) were estimated using noncompartment analysis and compared between the 2 arms. Forty‐eight patients were enrolled in the study, 24 in each arm. Patient demographics were comparable in both arms. Mean AUC0–inf for the generic and innovator formulations was 218.2 ± 19.18 and 223.6 ± 34.24 μg·h/mL, respectively, and mean Cmax was 119 ± 13.44 and 113 ± 7.26 μg/mL, respectively. Volume of distribution of pemetrexed was 17.5 and 27.6 L, clearance was 4.2 versus 4.72 L/h, and half‐life was 4.3 and 4.83 h in the 2 arms respectively. Both formulations showed comparable response rates (objective response of 45% versus 50% in the Pemgem and Alimta arms, respectively) and similar safety profiles. To conclude, Pemgem showed pharmacokinetic and safety profiles similar to Alimta. Substitution of Alimta with Pemgem will be cost‐effective and likely to yield comparable efficacy.
GRO‐TDC with gate‐switch‐based delay cell halving resolution limit
Authors: Park Jung‐Hyun , Jung Dong‐Hoon , Jung Seong‐Ook 
Published: March 2017

SummaryIn this paper, we propose a time‐to‐digital converter (TDC) with first‐order noise‐shaping. The proposed gated ring oscillator (GRO)‐TDC overcomes the limitation associated with GRO's intrinsic resolution by adopting two GROs, whose delay difference is equal to half the delay of a delay cell. The GRO is composed of 17 stages of a newly proposed delay cell, which utilizes a gate‐switched configuration to solve the charge redistribution problem. The proposed GRO‐TDC is designed using a 65‐nm process technology, with an area of 0.015 mm2 and a supply voltage of 1 V. The sampling rate and the effective resolution of the proposed GRO‐TDC are 50 MS/s and 1.22 ps, respectively. Finally, the proposed GRO‐TDC consumes a power of 9.08 and 2.41 mW in the calibration and conversion modes, respectively. Copyright © 2017 John Wiley & Sons, Ltd.
Experimental evidence that mycorrhizal nitrogen strategies affect soil carbon
Authors: Wurzburger Nina , Brookshire E. N. Jack 
Published: March 2017

AbstractMost land plants acquire nitrogen (N) through associations with arbuscular (AM) and ectomycorrhizal (ECM) fungi, but these symbionts employ contrasting strategies for N acquisition, which may lead to different stocks of soil carbon (C). We experimentally test this hypothesis with a mesocosm system where AM and ECM tree seedling roots, or their hyphae only, could access mineral soils with 13C‐ and 15N‐enriched organic matter. We quantified loss of soil C and N, plant uptake of N and new inputs of plant C to soil. We found that AM, but not ECM, seedlings reduced soil C relative to controls. Soil C loss was greater in the presence of roots relative to hyphae only for both AM and ECM seedlings, but was correlated with plant N uptake for AM seedlings only. While new plant C inputs stimulated soil C loss in both symbioses, we detected plant C inputs more frequently and measured higher rates of decomposer activity in soils colonized by AM relative to ECM seedlings. Our study experimentally demonstrates how mycorrhizal strategies for N can affect soil C and C:N, even at the scale of an individual plant. Such effects may contribute to broad patterns in soil C across terrestrial ecosystems.
Food‐web structure of willow‐galling sawflies and their natural enemies across Europe
Authors: Kopelke Jens‐Peter , Nyman Tommi , Cazelles Kévin , Gravel Dominique , Vissault Steve , Roslin Tomas 
Published: March 2017

AbstractCommunities consist of species and their interactions. They can thus be described as networks, with species as nodes and interactions as links. Within such networks, the diversity of nodes and the distribution of links may affect patterns of energy transfer between trophic levels, the dynamics of the system, and the outcome in terms of ecosystem functioning. To date, most descriptions of networks have focused on single or relatively few sites, and have oftentimes been built on poorly resolved nodes and links. Yet, comparisons of local interaction networks reveal variation in space and in time, thus spurring interest in methods and theory for understanding patterns, drivers, and consequences of this variation. Progress in this field relies on access to replicate samples of comparable food webs across large spatiotemporal scales, resolved to species rather than to compound nodes. Due to the massive efforts required, high‐quality data sets are still scarce. We created a data set on a single community type sampled across Europe: willow species (Salix), willow‐galling sawflies (Hymenoptera: Tenthredinidae: Nematinae: Euurina), and their natural enemies (hymenopteran parasitoids and coleopteran, lepidopteran, dipteran, and hymenopteran inquilines). Each sample was referenced in space and time, and each node resolved with the highest possible resolution, including taxonomic affinity, gall type (for herbivores), and mode of parasitism (for natural enemies). Galler survival and link structure were resolved by dissection and rearing of gall inhabitants. In total, the data set is based on 641 site visits over 29 years, and on 165,424 galls representing 96 herbivore nodes and 52 plant nodes. The dissections and rearings yielded 42,129 natural enemies belonging to 126 species, and revealed 1,173 different links. The spatiotemporal and taxonomic resolution of these data make them amenable to analyses of both ecological and evolutionary processes of network assembly. Thus, this data set will facilitate testing of important hypotheses in recent community theory, concerning, e.g., the sampling effort needed to adequately describe interaction structure within ecological communities, the impact of environmental conditions and biotic filters on the distribution of species and their interactions, and the relationship between the global “metaweb” and its local realizations.
Oral Cancer Screening Based on Methylation Frequency Detection in hTERT Gene Using Electrochemical Hybridization Assay via a Multi‐electrode Chip Coupled with Ferrocenylnaphthalene Diimide
Authors: Haraguchi Kazuya , Sato Shinobu , Habu Manabu , Yada Naomi , Hayakawa Mana , Takahashi Osamu , Yoshioka Izumi , Matsuo Kou , Tominaga Kazuhiro , Takenaka Shigeori 
Published: March 2017

AbstractFerrocenylnaphthalene diimide‐based electrochemical hybridization assay via a multi‐electrode chip was applied to detect the methylation frequency in the promoter region of human telomerase reverse transcriptase (hTERT) gene for clinical samples from tissues, local exfoliated oral cells from a lesion, or from entire oral cavity after their methylation specific PCRs. These methylation frequencies were increased with cancer progress as the following order: healthy volunteers, oral leukoplakia as precancerous lesion, and oral squamous cell carcinoma (OSCC). Operating characteristic analysis of the obtained current data doesn't only give excellent discrimination ability of OSCC, but also of oral leukoplakia from healthy volunteers for all samples. Sensitivity and specificity was 95 % and 90 %, respectively, which is a comparable with methods in practical use.
Improving outcomes in veterans with oropharyngeal squamous cell carcinoma through implementation of a multidisciplinary clinic
Authors: Light Tyler , Rassi Edward El , Maggiore Ronald J. , Holland John , Reed Julie , Suriano Kathleen , Stooksbury Marcelle , Tobin Nora , Gross Neil , Clayburgh Daniel 
Published: March 2017

AbstractBackgroundTreatment of head and neck cancer is complex, and a multidisciplinary clinic may improve the coordination of care. The value of a head and neck multidisciplinary clinic has not yet been established in oropharyngeal squamous cell carcinoma (SCC).MethodsA retrospective review was conducted of Veterans Affairs patients with oropharyngeal SCC undergoing concurrent chemoradiation before and after implementation of the head and neck multidisciplinary clinic.ResultsFifty‐two patients before and 54 patients after multidisciplinary clinic were included in this study. Age, tobacco use, and p16+ status were similar between groups. With multidisciplinary clinic, time to treatment decreased, and utilization of supportive services, including speech pathology, dentistry, and nutrition increased. The 5‐year disease‐specific survival rate increased from 63% to 81% (p = .043) after implementation of the multidisciplinary clinic. Multivariate analysis showed that disease stage (p = .016), p16 status (p = .006), and multidisciplinary clinic participation (p = .042) were predictors of disease‐specific survival.ConclusionImplementation of a multidisciplinary clinic improved care coordination and disease‐specific survival in patients with oropharyngeal SCC. © 2017 Wiley Periodicals, Inc. Head Neck 39: 1106–1112, 2017
Developing a risk stratification tool for audit of outcome after surgery for head and neck squamous cell carcinoma
Authors: Tighe David F. , Thomas Alan J. , Sassoon Isabel , Kinsman Robin , McGurk Mark 
Published: March 2017

AbstractBackgroundPatients treated surgically for head and neck squamous cell carcinoma (HNSCC) represent a heterogeneous group. Adjusting for patient case mix and complexity of surgery is essential if reporting outcomes represent surgical performance and quality of care.MethodsA case note audit totaling 1075 patients receiving 1218 operations done for HNSCC in 4 cancer networks was completed. Logistic regression, decision tree analysis, an artificial neural network, and Naïve Bayes Classifier were used to adjust for patient case‐mix using pertinent preoperative variables.ResultsThirty‐day complication rates varied widely (34%‐51%; P < .015) between units. The predictive models allowed risk stratification. The artificial neural network demonstrated the best predictive performance (area under the curve [AUC] 0.85).ConclusionEarly postoperative complications are a measurable outcome that can be used to benchmark surgical performance and quality of care. Surgical outcome reporting in national clinical audits should be taking account of the patient case mix.