bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of ultrasonographic diaphragmatic parameters in distinguishing diaphragmatic dysfunction in cats
Authors: Saisawart Phasamon , Sutthigran Somchin , Kasemsuwan Tanya , Sakulsirajit Run , Ritthikulprasert Sukullaya , Tachampa Kittipong , Thanaboonnipat Chutimon , Choisunirachon Nan 
Published: October 2024

Objectives Diaphragmatic dysfunction (DD) is often underdiagnosed in veterinary medicine. Various ultrasonographic diaphragmatic parameters, including diaphragmatic excursion (DE), diaphragmatic thickness (Tdi) and diaphragmatic thickening fraction (DTF), can be used to identify DD. We aimed to establish normal reference intervals in healthy cats, examine the effects of intrinsic factors on diaphragmatic parameters and evaluate the efficacy of these parameters in identifying DD in healthy and diseased cats. Methods This prospective observational study was conducted on 22 healthy cats and 12 diseased cats using ultrasonographic techniques to evaluate DE, Tdi and DTF. The correlation between diaphragmatic parameters and intrinsic factors, including sex, age, body weight and body condition score, was analysed using Pearson correlation analysis. Cut-off values for healthy and diseased cats were established using receiver operating characteristic curve analysis. Results The mean DEs of the left and right hemidiaphragms were 0.66 ± 0.16 and 0.64 ± 0.18 cm, respectively. The mean Tdis in the end-inspiration phase were 0.13 ± 0.03 and 0.12 ± 0.03 cm for the left and right hemidiaphragms, respectively, whereas in the end-expiration phase the mean Tdi was 0.07 ± 0.03 cm for both hemidiaphragms. The DTF ranges for the left and right hemidiaphragms were 23.90–122.1% and 38.80–107%, respectively. Intrinsic factors had no significant impact on DE, Tdi and DTF. The DE measurements for the left hemidiaphragm were more accurate for evaluating diaphragmatic function. To distinguish between healthy and diseased cats, the cut-off value, sensitivity and specificity were 0.458 cm (area under the curve [AUC] 0.846), 75.00% and 86.36%, respectively, for the left hemidiaphragm and 0.423 cm (AUC 0.704), 41.67% and 100%, respectively, for the right hemidiaphragm. Conclusions and relevance Normal reference values for DE, Tdi and DTF were established for healthy cats. DE, particularly in the left hemidiaphragm, obtained using routine ultrasonography, improves the efficacy of identifying DD and assists clinicians in the diagnosis and treatment of cats with cardiorespiratory disease.
Adherence to recommended follow-up in opportunistic versus non-opportunistic cervical screening: a registry-based cohort study from Denmark
Authors: Jørgensen Susanne F , Nielsen Mathilde L , Njor Sisse H 
Published: October 2024

Aim: Organised cervical screening programmes often allow for opportunistic screening. Previous studies have shown that adherence to follow-up protocols after cervical screening is poor. This study aims to investigate if non-adherence to recommended screening intervals – that is, opportunistic screening, is associated with adherence to follow-up after non-negative cervical screening. Methods: Using national registries we included 42,399 Danish women with a non-negative screening result from 2015 to 2017. Non-adherence was divided into insufficient and excessive follow-up. We calculated relative risks (RRs) of adherence to follow-up among different groups of opportunistically screened women, representing both too frequent and delayed screening participation, compared with non-opportunistically screened women. Results: Compared with non-opportunistically screened women, opportunistically screened women who were delayed 1–3 years on their screening schedule had a higher risk of no follow-up (RR 1.99) and insufficient follow-up (RR 1.10). Women who were delayed 3–7 years on their screening also had a higher risk of no follow-up (RR 1.92). Women who attended screening up to 6 months too early had a higher risk of insufficient follow-up (RR 1.08) as well as excessive follow-up (RR 1.39). Finally, women screened more than 6 months too early had a higher risk of all deviations, but most pronounced was the risk of excessive follow-up (RR 1.67). Conclusions: We found a higher risk of insufficient follow-up among women delayed in their screening schedule, and a higher risk of excessive follow-up among those with frequent screening. Screening participation behaviour seems to transfer to follow-up adherence. This underscores the important role of general practitioners in acting as gatekeepers in cervical screening follow-up.
Clinical significance of upper arm motor nerve conduction velocity in cubital tunnel syndrome
Authors: Uehara Kazuya , Hashimoto Takahiro , Yukata Kiminori , Imajo Yasuaki , Funaba Masahiro , Fujii Kenzo , Sakai Takashi 
Published: October 2024

Background Retrograde denervation occurs in severe entrapment neuropathy. This study aimed to investigate changes in motor nerve conduction velocity (MNCV) at the upper arm, specifically proximal to the elbow, in cubital tunnel syndrome (CuTS) and to correlate these changes with preoperative severity and postoperative outcomes. Methods We retrospectively reviewed 95 elbows with 81 patients with CuTS for preoperative severity, and then 67 elbows with 60 of these patients who underwent anterior subcutaneous transposition surgery for postoperative outcome, classified into favorable and unfavorable groups according to Messina grade. Results The reduction of upper arm MNCV was correlated with aging, decreased compound muscle action potentials of abductor digiti minimi muscle, loss of sensory nerve action potentials, and modified McGowan grade. Postoperative assessment revealed lower MNCV values in the “unfavorable” group compared to the “favorable” group at 1 month, 6 months, and the last follow up. At each time point, the optimal cut-off value of upper arm MNCV for predicting postoperative outcomes was 54.1 m/s. Discussion Upper arm MNCV might be a useful predictor of poor surgical outcome. Ulnar nerve MNCV at the upper arm should be measured alongside routine assessment of MNCV at the elbow and forearm, especially in clinically severe cases considering surgery.
Validation of a parent-proxy, obesity-specific Health-Related Quality of Life measure - sizing them up - in Singaporean adolescents seeking obesity treatment
Authors: Ng Wang Chin Eileen , Khaider Khairunisa Binte , Fan Qiao , Chew Chu Shan Elaine 
Published: October 2024

Background Condition-specific measures of Health-Related Quality of Life (HRQoL) are more sensitive than generic tools. Sizing Them Up (STU) is a parent-proxy, obesity-specific HRQoL measure which was found to be reliable and valid internationally, but has yet to be utilised in Singapore. Objectives Our study aims to i) validate STU in adolescents seeking obesity therapy in KK Women’s and Children’s Hospital (KKH); and ii) determine demographic factors associated with STU scores. Methods We recruited 91 adolescents aged between 11 and 17 years old and their parents from KKH between June 2022 and January 2023. Parents completed STU and the generic Pediatric Quality of Life Inventory (PedsQL) upon recruitment, and repeated STU at least 2 weeks later. Results 65% of participants were males, with a mean Body Mass Index (BMI) of 32.3 ± 5.1 kg/m2 and mean age of 13.6 ± 1.8 years old. STU demonstrated strong internal consistency (overall Cronbach’s α = 0.85) and moderate test-retest reliability (intraclass correlation coefficients = 0.30-0.70). There was high correlation between STU scores and i) the adolescents’ BMI; and ii) equivalent domains of PedsQL, suggesting moderate to strong construct and convergent validity respectively. Indian adolescents, lower parental education level and single parents were associated with poorer STU scores. Conclusion STU is a reliable, valid obesity-specific HRQoL measure in a local clinical population of adolescents undergoing obesity therapy, and can help clinicians to understand parents’ impression of the impact of obesity on their child. Knowing the demographic variables associated with STU can enable clinicians to identify adolescents whose HRQoL are disproportionately affected by obesity.
Patients with Waldenström macroglobulinemia have impaired platelet and coagulation function
Authors: Brysland Simone A. , Talaulikar Dipti , Hicks Sarah M. , Hearn James I. , Ali Sidra A. , Maqbool Muhammad Gohar , Mokoonlall Mridula , Bhoopalan Vijay , Kaur Amandeep , Thong Yee Lin , Andrews Robert K. , Whisstock James C. , Crispin Philip J. , Gardiner Elizabeth E. 
Published: October 2024

Abstract
Clinical features in patients with the B-cell lymphoma, Waldenström macroglobulinemia (WM), include cytopenias, immunoglobulin M (IgM)–mediated hyperviscosity, fatigue, bleeding, and bruising. Therapeutics such as Bruton's tyrosine kinase inhibitors (BTKis) exacerbate bleeding risk. Abnormal hemostasis arising from platelet dysfunction, altered coagulation or vascular impairment have not yet been investigated in patients with WM. Therefore, this study aimed to evaluate hemostatic dysfunction in samples from these patients. Whole blood (WB) samples were collected from 14 patients with WM not receiving therapy, 5 patients receiving BTKis and 15 healthy donors (HDs). Platelet receptor levels and reticulation were measured by flow cytometry, plasma thrombin generation with or without platelets by fluorescence resonance energy transfer assay, WB clotting potential by rotational thromboelastometry, and plasma soluble glycoprotein VI (sGPVI) and serum thrombopoietin (TPO) by enzyme-linked immunosorbent assay. Donor platelet spreading, aggregation, and ability to accelerate thrombin generation in the presence of WM-derived IgM were assessed. WM platelet receptor levels, responses to physiological agonists, and plasma sGPVI were within normal ranges. WM platelets had reduced reticulation (P = .0012) whereas serum TPO levels were increased (P = .0040). WM plasma displayed slower thrombin generation (P = .0080) and WM platelets contributed less to endogenous thrombin potential (ETP; P = .0312). HD plasma or platelets incubated with IgM (50-60 mg/mL) displayed reduced spreading (P = .0002), aggregation (P &lt; .0001), and ETP (P = .0081). Thus, alterations to thrombin potential and WB coagulation were detected in WM samples. WM IgM significantly impaired hemostasis in vitro. Platelet and coagulation properties are disturbed in patients with well-managed WM.
Skeletal myotubes expressing ALS mutant SOD1 induce pathogenic changes, impair mitochondrial axonal transport, and trigger motoneuron death
Authors: Martínez Pablo , Silva Mónica , Abarzúa Sebastián , Tevy María Florencia , Jaimovich Enrique , Constantine-Paton Martha , Bustos Fernando J. , van Zundert Brigitte 
Published: October 2024

AbstractAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the loss of motoneurons (MNs), and despite progress, there is no effective treatment. A large body of evidence shows that astrocytes expressing ALS-linked mutant proteins cause non-cell autonomous toxicity of MNs. Although MNs innervate muscle fibers and ALS is characterized by the early disruption of the neuromuscular junction (NMJ) and axon degeneration, there are controversies about whether muscle contributes to non-cell-autonomous toxicity to MNs. In this study, we generated primary skeletal myotubes from myoblasts derived from ALS mice expressing human mutant SOD1G93A (termed hereafter mutSOD1). Characterization revealed that mutSOD1 skeletal myotubes display intrinsic phenotypic and functional differences compared to control myotubes generated from non-transgenic (NTg) littermates. Next, we analyzed whether ALS myotubes exert non-cell-autonomous toxicity to MNs. We report that conditioned media from mutSOD1 myotubes (mutSOD1-MCM), but not from control myotubes (NTg-MCM), induced robust death of primary MNs in mixed spinal cord cultures and compartmentalized microfluidic chambers. Our study further revealed that applying mutSOD1-MCM to the MN axonal side in microfluidic devices rapidly reduces mitochondrial axonal transport while increasing Ca2 + transients and reactive oxygen species (i.e., H2O2). These results indicate that soluble factor(s) released by mutSOD1 myotubes cause MN axonopathy that leads to lethal pathogenic changes.
Modelling interrelations between C-ITS impact categories: a system-dynamics approach using causal loop diagrams
Authors: Walch Manuel , Neubauer Matthias , Schildorfer Wolfgang , Schirrer Alexander 
Published: October 2024

AbstractThe growing number of connected vehicles has led to an increased focus on Vehicle-to-Everything (V2X) communication in the field of transport research. This communication paradigm facilitates cooperation between vehicles and infrastructure to address traffic challenges such as efficiency, sustainability and safety. The development and standardisation of such Cooperative Intelligent Transport Systems (C-ITS) has been pursued in several projects. Beyond technical considerations, assessing the effect of these applications in terms of various impact categories is of paramount importance. However, existing research tends to examine impact categories such as efficiency, sustainability, safety, psychological or socioeconomic impacts separately, often overlooking potential interactions and interdependencies. This approach is inadequate as impacts on one category can have both cascading effects on others and rebound effects. To address this gap, this paper proposes a system dynamics approach using Causal Loop Diagrams (CLD) to illustrate the interconnectedness of impact categories and the potential impacts of C-ITS services. By depicting general relationships, interdependencies and feedback loops between impact category elements, the model accommodates the introduction of single or multiple C-ITS services as separate modules, allowing an analysis of their combined effects on the overall system. To this end, two use cases demonstrate the applicability of the developed CLD and illustrate some of the multiple interrelations between the effects of C-ITS services. The results of this paper support road operators and researchers when setting up the impact assessment of C-ITS services by revealing the dynamic and intertwined nature of different impact categories.
Burden of vaccine-preventable diseases in adults (50+) in the United States: a retrospective claims analysis
Authors: Hartmann Maximilian , Servotte Nathalie , Aris Emmanuel , Doherty T. Mark , Salem Ahmed , Beck Ekkehard 
Published: October 2024

Abstract
Background
In adults aged 50 + years, vaccine-preventable diseases (VPDs) pose a significant health burden and can lead to additional ‘downstream effects’ of infection beyond the acute phase e.g., increasing the risk for non-communicable disease and exacerbating chronic conditions. The aim was to understand and quantify the burden of VPD downstream effects in hospitalised adults in the United States.

Methods
This retrospective observational study analysed hospitalisation claims data (2016–2019) with 1-year follow-up, in adults with a VPD diagnosis versus matched controls (using Optum’s de-identified Clinformatics Data Mart Database). Outcomes included mortality; increase in Charlson Comorbidity Index (CCI) score; new diagnosis of comorbidities; and loss of independence (defined by need for home health/home care and/or move to long-term facility).

Results
Mortality was significantly increased in VPD cases versus controls at 30-day (risk ratio [RR] of 4.08 [95% CI 3.98–4.18]) and 1-year follow-up (RR 2.76 [2.73–2.80]). Over a 1-year follow-up period, morbidity increased following VPD hospitalisation: 65–86% of VPD cases had new comorbidities diagnosed (versus 13–41% of controls); with a significantly higher mean increase in CCI score versus baseline (3.23 in VPD cases versus 0.89 in controls, p < 0.001). Adults were observed to experience a worsening of their health status and were less likely to return to their original health state. In addition, 41% of VPD cases had a loss of independence following hospitalisation versus 12% of controls; as seen by an increased need for home assistance (in 25% versus 9% of controls) and/or a move to a long-term care facility (in 29% versus 6% of controls).

Conclusions
This analysis suggests that VPD hospitalised cases suffer significantly worse clinical outcomes than controls, with downstream effects that include increased mortality and morbidity, and greater loss of independence. Evidence on potential downstream effects of infection is relatively new, and this additional burden is generally not considered in vaccine decision-making. More research is needed to disentangle the effect of VPDs on new comorbidities versus the natural course of the condition. Increasing awareness among adults, healthcare providers and decision makers could help to increase adult vaccination coverage, and reduce the clinical burden of VPDs.
Evaluating suicide prevention gatekeeper training designed to identify and support people from asylum-seeking and refugee backgrounds
Authors: Hart Steven MacDonald , Colucci Erminia , Marzano Lisa 
Published: October 2024

Abstract
Background
Suicide-related behaviours and individual risk factors for suicide differ between ethnicities and demonstrate additional variation based on voluntary and forced migration. People forcibly displaced by violence and conflict, such as those seeking asylum and refugees, are likely to face stressors that can increase suicide risk. Research into evidenced-based suicide prevention strategies among people from asylum-seeking and refugee backgrounds is scarce. However, early, contextually-appropriate, identification and intervention may be a promising way to facilitate support for people in these groups. This research proposes that a contextually-responsive gatekeeper training is an appropriate strategy to increase the identification and support for people from asylum-seeking and refugee backgrounds.

Methods
The present article relates to the statistical findings of a larger mixed-method study used to validate and refine a contextually-responsive gatekeeper training program. The qualitative results of this research will be published separately. The outcome measures – knowledge about suicide in multicultural contexts, attitudes towards suicide and prevention, and self-efficacy to intervene were measured quantitatively, adopting a similar pre- and post-training procedure used in previous training evaluations. Using Generalised Estimating Equations, statistical comparisons were made between three identical self-report surveys completed by participants across three consecutive time points – pre-training, immediately post-training, and three months following training completion – known in this investigation as time-point zero (T0), time-point one (T1), and time-point two (T2). Lastly, during the T2 follow-up, additional open-ended questions were included to understand which areas of training they feel prepared them effectively and how the program could have better prepared them to intervene.

Results
A total of 28 participants took part in the study. Quantitative analysis indicated the program’s capacity to exert a significant favourable and lasting influence on knowledge about suicide and self-efficacy to intervene. In addition, follow-up measurements suggest that the content delivered to participants transferred effectively into real-world suicide prevention behaviours.

Conclusions
Findings suggest that tailored suicide prevention training can have a significant influence on knowledge about suicide in multicultural contexts, self-efficacy to intervene in a crisis, and that course content translates effectively into real-world suicide prevention behaviour. Modifying training practices, based on feedback from contextually-experienced attendees, appears to be a pivotal factor in promoting the support of people from asylum-seeking and refugee backgrounds.
Impact of low dose inhaled nitric oxide treatment in spontaneously breathing and intubated COVID-19 patients: a retrospective propensity-matched study
Authors: Isha Shahin , Balasubramanian Prasanth , Hanson Abby J. , Jonna Sadhana , Raavi Lekhya , Khadka Subekshya , Vasudhar Ananya , Sinclair De Frias Jorge , Jenkins Anna , Balavenkataraman Arvind , Tekin Aysun , Bansal Vikas , Reddy Swetha , Caples Sean M. , Khan Syed Anjum , Jain Nitesh K. , LaNou Abigail T. , Kashyap Rahul , Cartin-Ceba Rodrigo , Milian Ricardo Diaz , Venegas Carla P. , Shapiro Anna B. , Bhattacharyya Anirban , Chaudhary Sanjay , Kiley Sean P. , Quinones Quintin J. , Patel Neal M. , Guru Pramod K. , Moreno Franco Pablo , Sanghavi Devang K. 
Published: October 2024

Abstract
Background
The benefit of Inhaled nitric oxide (iNO) therapy in the setting of COVID-19-related ARDS is obscure. We performed a multicenter retrospective study to evaluate the impact of iNO on patients with COVID-19 who require respiratory support.

Methods
This retrospective multicenter study included COVID-19 patients enrolled in the SCCM VIRUS COVID-19 registry who were admitted to different Mayo Clinic sites between March 2020 and June 2022 and required high-flow nasal cannula (HFNC), non-invasive ventilation (NIV), or invasive mechanical ventilation (IMV). Patients were included in the ‘spontaneously breathing’ group if they remained non-intubated or were initiated on an HFNC (± NIV) before intubation. Patients who got intubated without prior use of an HFNC (± NIV) were included in the ‘intubated group.’ They were further divided into categories based on their iNO usage. Propensity score matching (PSM) and inverse propensity of treatment weighting (IPTW) were performed to examine outcomes.

Results
Among 2767 patients included in our analysis, 1879 belonged to spontaneously breathing (153 received iNO), and 888 belonged to the intubated group (193 received iNO). There was a consistent improvement in FiO2 requirement, P/F ratio, and respiratory rate within 48 h of iNO use among both spontaneously breathing and intubated groups. However, there was no significant difference in intubation risk with iNO use among spontaneously breathing patients (PSM OR 1.08, CI 0.71–1.65; IPTW OR 1.10, CI 0.90–1.33). In a time-to-event analysis using Cox proportional hazard model, spontaneously breathing patients initiated on iNO had a lower hazard ratio of in-hospital mortality (PSM HR 0.49, CI 0.32–0.75, IPTW HR 0.40, 95% CI 0.26–0.62) but intubated patients did not (PSM HR: 0.90; CI 0.66–1.24, IPTW HR 0.98, 95% CI 0.73–1.31). iNO use was associated with longer in-hospital stays, ICU stays, ventilation duration, and a higher incidence of creatinine rise.

Conclusions
This retrospective propensity-score matched study showed that spontaneously breathing COVID-19 patients on HFNC/ NIV support had a decreased in-hospital mortality risk with iNO use in a time-to-event analysis. Both intubated and spontaneously breathing patients had improvement in oxygenation parameters with iNO therapy but were associated with longer in-hospital stays, ICU stays, ventilation duration, and higher incidence of creatinine rise.