bluuebunny/crossref_metadata_2025_split huggingface dataset:
The growth rate of Nile tilapia Oreochromis niloticus fry fed on fermented Lemna sp. meal
Authors: Rostika Rita , Andriani Yuli , Abram Arthur Henry , Vinasyiam Apriana 
Published: January 2018

ABSTRACT This research aimed to evaluate the supplementation of fermented Lemna sp. meal (FLM) in the artificial diet on the growth rate of Nile tilapia Oreochromis niloticus. The research design used was complete randomized design consisting of four treatments; A (0% FLM), B (20% FLM), C (30% FLM), and D (40% FLM) in triplicates. The parameters observed were the nutritional composition of LFM, daily growth rate (DGR), feed conversion ratio (FCR), survival rate (SR), and water quality. Fermentation with Effective Microorganism 4 (EM4) has increased crude protein and crude lipid of FLM respectively 5.60% and 5.76%. However it decreased the crude fiber of FLM up to 15.27%. The result suggested that supplementation of FLM 0–40% in the artificial diet could give DGR 0.81–1.20%, FCR 2.48–2.97; and SR 72%–84% in Nile tilapia. The addition of 40% LFM showed the best result among all treatments. Key words: Lemna meal, EM4, fermentation, Nile tilapia fry ABSTRAK Tujuan dari penelitian ini adalah untuk mengevaluasi penggunaan tepung Lemna sp. fermentasi (TLF) pada pakan buatan terhadap laju pertumbuhan ikan nila Oreochromis niloticus. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) terdiri atas empat perlakuan, yaitu A (0% TLF), B (20% TLF), C (30% TLF), dan D (40% TLF) dengan masing-masing tiga ulangan. Parameter yang diamati dalam penelitian antara lain nilai nutrisi TLF, laju pertumbuhan harian (LPH) ikan nila, rasio konversi pakan (RKP), sintasan (SR), dan kualitas air. Fermentasi dengan Effective Microorganism 4 (EM4) meningkatkan protein kasar dan lemak kasar TLF sebesar masing-masing 5,60% dan 6,76%, akan tetapi menurunkan serat kasar hingga 15,27%. Hasil penelitian menunjukkan bahwa pemberian TLF 0–40% dalam pakan komersial menghasilkan LPH 0,81–1,20%; RKP 2,48– 2,97; dan SR 72%–84%. Pemberian TLF sebanyak 40% merupakan perlakuan yang menunjukkan hasil terbaik. Kata kunci: tepung Lemna, EM4, fermentasi, benih ikan nila
Aromatase gene expression and masculinization of Nile tilapia immersed in water 36 °C containing 17α-methyltestosterone
Authors: Fauzan Agung Luthfi , Soelistyowati Dinar Tri , Junior Muhammad Zairin , Hardiantho Dian , Setiawati Mia , Alimuddin , 
Published: January 2018

ABSTRACT Immersion of undifferentiated larval tilapia in high temperature and 17α-methyltestosterone (MT) cab increase the male ratio. However, the effectiveness of immersion in high temperature of water containing MT remains to be evaluated. The purposes of this study were: 1) evaluate the male ratio, growth, and survival of tilapia, and 2) analyze the aromatase brain-type gene expression level in tilapia after immersing in high temperature (36 °C) containing MT at 2 mg/L for four hour with single and double immersion. Aromatase gene expression was analyzed by semi-quantitative RT-PCR (sqRT-PCR) method. The result showed that higher monosex male ratio was obtained by single immersion of MT at 36 °C at room temperature. Gene expression level of aromatase brain-type was lower on single immersion and increased significantly at second immersion compared to control (immersion at room temperature without MT). Immersion using MT and high temperature had no significant effect on fish survival. However the specific growth rate and fish biomass were higher than control. Thus, monosex male tilapia can be produced by single immersion of undifferentiated larvae at 36 °C temperature containing MT. Keywords: male ratio, aromatase, Oreochromis niloticus, temperature, 17α-methyltestosterone ABSTRAK Perendaman larva ikan nila yang belum terdeferensiasi kelaminnya dengan suhu tinggi dan hormon 17α-metiltestosteron (MT) dapat meningkatkan nisbah kelamin jantan. Tetapi, efektivitas perendaman menggunakan MT pada suhu tinggi belum diteliti. Tujuan dari penelitian ini adalah 1) mengevaluasi nisbah kelamin jantan, pertumbuhan, dan kelangsungan hidup ikan nila, dan 2) menganalisis ekspresi gen aromatase tipe-otak pada ikan direndam menggunakan MT dengan dosis 2 mg/L selama empat jam sebanyak satu dan dua kali perendaman pada suhu 36 °C. Ekspresi gen aromatase dianalisis menggunakan metode RT-PCR semi-kuantitatif (sqRT-PCR). Hasil penelitian menunjukkan bahwa kombinasi perendaman MT satu kali pada suhu 36 °C lebih tinggi menghasilkan ikan nila jantan monoseks dibandingkan perendaman MT satu kali pada suhu ruang. Tingkat ekspresi gen aromatase tipe otak pada perendaman satu kali lebih rendah, dan meningkat secara signifikan pada perendaman kedua dibandingkan dengan kontrol (perendaman pada suhu ruang tanpa MT). Perendaman larva menggunakan MT dan suhu 36 °C tidak berpengaruh nyata terhadap kelangsungan hidup, tetapi laju pertumbuhan spesifik dan biomassa ikan perlakuan tersebut lebih tinggi daripada kontrol. Dengan demikian, ikan nila jantan monoseks dapat diproduksi dengan perendaman satu kali pada larva yang belum terdeferensiasi jenis kelaminnya menggunakan MT pada suhu 36 °C. Kata kunci: rasio jantan, aromatase, Oreochromis niloticus, suhu, 17α-metiltestosteron
The population growth and the nutritional status of Moina macrocopa feed with rice bran and cassava bran suspensions
Authors: Mubarak Ahmad Shofy , Jusadi Dedi , Junior Muhammad Zairin , Suprayudi Muhammad Agus 
Published: January 2018

ABSTRACT Moina macrocopa culture density can be improved by optimizing the fecundity, and somatic growth through the regulation of quality and quantity of feed. The purpose of this study were to determined how to use effectively the rice bran and cassava bran Manihot utilisima suspension on Moina, based on population, neonates production, adult percentage, biomass, metabolisme and nutritional state. In this study, Moina were cultured for eighth days using four concentrations of rice bran suspension and three concentrations of cassava suspension. This research found that M. macrocopa culture with rice bran suspension has higher population, neonates production, adult percentage and biomass than its culture with cassava bran suspension (P<0.05). This study also found that Moina culture with rice bran suspension has higher total value of RNA, total value of DNA, the ratio RNA/ DNA, FCR, and concentration of protein and amino acid than Moina culture with cassava bran suspension. Treatment D with the initial rice bran suspension concentration was 0.3 mL/L and was increased starting the second day and the end concentration on the eighth day was 1.2 mL/L has highest peak population of Moina 17,975 ind/L in seventh day, weight wet biomass 439 mg/L in eighth day and lower FCR 0.94. Keywords: suspension, rice bran, cassava, population, ratio RNA/DNA ABSTRAK Kepadatan populasi dalam budidaya Moina macrocopa dapat ditingkatkan dengan mengoptimalkan fekunditas dan pertumbuhan somatik melalui pengaturan kualitas dan kuantitas pakan. Penelitian ini bertujuan untuk menganalisis efektifitas pengunaan pakan suspensi dedak dan tepung ketela pohon Manihot utilisima dalam budidaya M. macrocopa terhadap populasi, produksi anak per induk, persentase dewasa, biomasa, FCR, dan metabolismenya (asam amino, DNA, RNA, dan RNA/DNA). Di dalam penelitian ini, M. macrocopa dibudidayakan selama delapan hari menggunakan empat konsentrasi suspensi dedak dan tiga konsentrasi suspensi tepung ketela pohon. Hasil penelitian ini menunjukkan bahwa, budidaya M. macrocopa dengan pakan suspensi dedak menghasilkan populasi, produksi anak/induk, persentase dewasa dan biomasa yang lebih tinggi dibandingkan budidaya Moina dengan pakan suspensi ketela pohon (P<0,05). Budidaya M. macrocopa dengan pakan suspensi dedak juga menghasilkan total RNA, total DNA dan nisbah RNA/DNA, konsentrasi protein, dan asam amino yang lebih tinggi dibandingkan Moina dengan pakan suspensi ketela pohon. Perlakuan D dengan pakan suspensi dedak awal 0,2 mL/L dan meningkat mulai hari kedua dengan konsentrasi hari kedelapan 1,2 mL/L menghasilkan puncak populasi tertinggi pada hari ketujuh sebanyak 17.975 ind/L, berat basah biomasa hari kedelapan kultur 439 mg/L, dan FCR yang rendah yaitu 0,94. Kata kunci: suspensi, dedak, ketela pohon, populasi, nisbah RNA/DNA
Suitability analysis of Lampung Bay waters for grouper Epinephelus sp. farming activities
Authors: Yulianto Herman , Hartoko Agus , Anggoro Sutrisno , Hasani Qadar , Mulyasih Dwi , Delis Putu Cinthia 
Published: January 2018

ABSTRACT Grouper Epinephelus sp. farming activities in Lampung Bay is limited to an area of 77 hectares, while areas that are potential to be used for grouper farming are still very wide. Therefore, this study aimed to assess the suitability of Lampung Bay waters for grouper farming activities. The study was conducted in 20 stations with ecological preference considerations. The parameters observed were physicochemical (water depth, temperature, water transparency, the load of suspended solids, pH, dissolved oxygen, salinity, nitrate, and phosphate) and biological parameters (phytoplankton abundance and chlorophyll-a concentration). After the data were completely obtained, the data were processed into suitability matrix resulting in scores that will be grouped into four classes, namely S1 (highly suitable), S2 (moderately suitable), S3 (marginally suitable), N (not suitable). The geostatistical model was used to perform the earth’s surface mapping based on biotic and abiotic parameters that were analyzed. Based on the results of the analysis in this study, the conditions of Lampung Bay waters were suitable for grouper farming activities. Marine area that could be used for grouper farming was 33,847.12 hectares (S1: 15,712.6 ha, S2: 13,294.7 ha and S3: 4,209.82 ha) in the area around Puhawang Island, Kelagian Island, Maitem Island, Tegal Island to Hurun Bay. Keywords: Lampung Bay, grouper, suitability analysis, fish farming ABSTRAK Kegiatan budidaya ikan kerapu Epinephelus sp. di Teluk Lampung masih terbatas pada lahan seluas 77 hektar, sedangkan lahan yang berpotensi digunakan untuk budidaya ikan kerapu masih sangat luas. Oleh karena itu, penelitian ini bertujuan mengkaji kesesuaian perairan Teluk Lampung untuk kegiatan budidaya ikan kerapu. Penelitian dilakukan di 20 stasiun dengan pertimbangan ecological preference. Parameter yang diamati yaitu parameter fisika-kimia perairan (kedalaman, suhu, kecerahan, muatan padatan tersuspensi, pH, oksigen terlarut, salinitas, nitrat, dan fosfat) serta parameter biologi (kelimpahan fitoplankton dan konsentrasi klorofil-a). Setelah data parameter terpenuhi, data diolah ke dalam matriks kesesuaian sehingga menghasilkan skor yang akan dikelompokkan ke dalam empat kelas, yaitu S1 (highly suitable), S2 (moderately suitable), S3 (marginally suitable), N (not suitable). Model geostatistik digunakan untuk melakukan pemetaan permukaan bumi berdasarkan data parameter biotik dan abiotik yang dianalisis. Berdasarkan hasil analisis pada penelitian ini, kondisi perairan Teluk Lampung sesuai untuk kegiatan budidaya ikan kerapu. Luas perairan laut yang dapat digunakan untuk kegiatan budidya ikan kerapu yaitu, 33.847,12 hektar (S1: 15.712,6 ha, S2: 13.294,7 ha dan S3: 4.209,82 ha) di daerah sekitar Pulau Puhawang, Pulau Kelagian, Pulau Maitem, Pulau Tegal hingga Teluk Hurun. Kata kunci: Teluk Lampung, ikan kerapu, analisis kesesuaian, budidaya
Optimization of stocking density in intensification of mud crab Scylla serrata cultivation in the resirculation system
Authors: Hastuti Yuni Puji , Nirmala Kukuh , Rusmana Iman , Affandi Ridwan , Kuntari Wahyu Budi 
Published: January 2018

ABSTRACT This study aimed to determine optimum stocking density of mud crab Scylla serrata through the applied of different stocking density in every treatment in recirculation system. Experimental design used was complete randomized design (CRD) with three density treatments which were 5 (P1), 10 (P2), and 15 ind/container (P3). All treatments replicated three times. The crab with the average of body weight 150 g/ind cultured in a plastic box (40×30×30 cm). Crab was cultured within 60 days and were fed two times a day by at satiation method. The result showed that P2 treatment gave the best result of mangrove crabs production performance among all treatments with 73.33±5.77% survival rate, 0.68±0.01 g/ind/day absolute growth rate and food conversion ratio 10.11±0.01. Treatment P1 gave the good response of stress, it indicated by the lowest glucose of all tretamnets at the level of 31.91 mg/dL in the end of treatment periods. The water quality during study period was fluctuative as affected by different stocking density in the treatments. Keywords: mud crab, stocking density, production performance ABSTRAK Penelitian ini bertujuan untuk menentukan padat tebar optimal kepiting bakau Scylla serrata melalui penerapan kepadatan tebar yang berbeda pada setiap perlakuan dalam sistem resirkulasi. Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan tiga perlakuan yaitu 5 (P1), 10 (P2), dan 15 ekor/wadah pemeliharaan (P3) dengan tiga ulangan. Kepiting bakau yang digunakan memiliki berat rata-rata 150 g/ekor. Wadah pemeliharaan yang digunakan selama pemeliharaan adalah kontainer plastik yang berukuran 40×30×30 cm. Pemeliharaan kepiting bakau dilaksanakan selama 60 hari dan diberikan pakan berupa ikan rucah dua kali sehari secara at satiation. Penelitian menunjukkan bahwa perlakuan P2 memberikan hasil kinerja produksi terbaik dibandingkan perlakuan lainnya dengan nilai kelangsungan hidup 73,33±5,77%, laju pertumbuhan mutlak 0,68±0,01 g/ekor/hari, dan rasio konversi pakan 10,11±0,01. Perlakuan P1 menunjukkan respons stres yang baik dengan memiliki nilai kadar glukosa paling rendah dari perlakuan lain yaitu 31,91 mg/dL pada akhir masa pemeliharaan. Kualitas air selama penelitian memiliki nilai yang fluktuatif di setiap perlakuan sebagai efek adanya perbedaan jumlah padat tebar setiap wadahnya Kata kunci: kepiting bakau, padat tebar, kinerja produksi
Vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in its fry against Aeromonas hydrophila
Authors: Sukenda Sukenda , Carman Odang , Rahman Rahman , Hidayatullah Dendi , Yumaidawati Nurfitriani Siti 
Published: January 2018

ABSTRACT The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against Aeromonas hydrophila. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m3). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry. Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P<0.05) compared to the control. Relative percent survival of offspring at 5, 10, and 15 days were 78.26%, 70.59%, and 65.52%, respectively. As a conclusion, vaccination in tilapia broodstock was effective to improve specific and non-specific immunity, and protect fry tilapia from A. hydrophila infection through maternal immunity. Keywords: vaccination, antibody, maternal immunity, tilapia, Aeromonas hydrophila ABSTRAK Penelitian ini bertujuan untuk menganalisis efikasi vaksinasi pada induk nila dengan vaksin sel utuh dan ketahanan benih yang dihasilkan terhadap Aeromonas hydrophila. Ikan nila stain Nirwana yang digunakan dalam penelitian memiliki bobot rata-rata 185±13,23 g dan ikan dipelihara dalam kolam (2,5×2,5×1 m3). vaksinasi dilakukan melalui penyuntikan intraperitoneal dengan dosis 0,1 mL/ikan, sementara itu ikan kontrol disuntik dengan phosphate buffered saline (PBS). Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Tingkat antibodi diukur dengan menggunakan metode indirect enzyme-linked immunosorbent assay (ELISA) pada induk, telur dan benih. Uji tantang pada benih dilakukan pada umur 5, 10, dan 15 hari. Hasil penelitian menunjukan bahwa vaksinasi pada induk nila secara signifikan dapat meningkatkan level antibodi pada induk, telur, dan benih (P<0,05) dibandingkan dengan kontrol. Kelangsungan hidup relatif pada benih berumur 5, 10, dan 15 hari masing-masing adalah 78,26%; 70,59%; dan 65,52%. Sebagai kesimpulan vaksinasi pada induk nila efektif dalam memperbaiki imunitas spesifik dan non spesifik serta melindungi benih dari infeksi A. hydrophila melalui imunitas maternal. Kata kunci: vaksinasi, antibodi, imunitas maternal, ikan nila, Aeromonas hydrophila
Creative People as a New Source of Competitive Advantage in Creative Industry
Authors: Astuty Eriana , Suryana Suryana 
Published: January 2018

Today’s development of creative industries in the economy of Indonesia has shown a significant improvement. Creative industries have contributed in improving the growth of Gross Domestic Product (GDP), employment opportunities, and national industries. Unfortunately, those improvements are not in line with their internal developments. The purpose of this study is to find the answer of the gap phenomenon that occured. It is found that only three sub-sectors out of 15 have positive contributions as mentioned above. They include craft, fashion, and culinary. Primary data were obtained through a questionnaire survey and observation carried out in batik and woven-fabric business units in 6 provinces in Indonesia. By conducting survey as the research methodology in which the data were evaluated using statistic tools such as SPSS and AMOS 20, it is found that creative people as renewable resources have been able to improve Indonesia competitive advantage in crafts industries by reflecting local culture and heritage through creativity on various innovations.
The Potential of Using Cr3+/Salt-Tolerant Polymer Gel for Well Workover in Low-Temperature Reservoir: Laboratory Investigation and Pilot Test
Authors: Jia Hu , Chen Hao 
Published: January 2018

Summary
Using mature Cr3+/partially hydrolyzed polyacrylamide (HPAM) gel can reduce filtration for water shutoff in the fractured reservoir. Whether the mature gel can act as a fluid-loss-control pill for well-workover operation is worth investigating. In this paper, we start a systematic experimental study to reveal the physical process and fluid-loss-control mechanism of the Cr3+/KYPAM (salt-tolerant polymer) gel used for overbalanced well workover. The polymer gel used in this study is formulated with a combination of 0.4 to 0.6 wt% KYPAM and added 0.02 to 0.04 wt% chromium acetate, which can provide a gelation time between 2 and 4 hours, and with a maximum gel strength of Code G at a temperature of 30°C. Results show that the mature Cr3+/KYPAM gel can withstand positive pressure of 10 MPa for a period of 120 minutes with average fluid-loss volume of 15 cm3 for the core permeability between 9.18 and 217 md, indicating a favorable fluid-loss-control performance. The regained-permeability recovery can reach up to 85% for different core permeabilities. Scanning-electron-microscope (SEM) pictures show that a dense structure was formed in the gel filter cake during fluid-loss experiment. The wettability results show that the core has a greater potential to increase its water-wet ability after interacting with Cr3+/KYPAM mature gel. Field test shows that a small amount of gel leakoff was observed during each reperforation process, whereas water cut decreased from 89.1 to 52.1% and oil production increased from 0.15 to 1.11 m3/d. This study suggests that the mature Cr3+/KYPAM gel can act as a fluid-loss-control pill in high-water-cut oil wells, which can provide an avenue to bridge the design philosophy of well workover and water shutoff.
Development of Acid Fracturing Model for Naturally Fractured Reservoirs
Authors: Ugursal A. , Zhu D. , Hill A. D. 
Published: January 2018

Abstract
During an acid fracturing treatment in a carbonate reservoir, acid is injected into the formation creating hydraulic fractures and opening existing natural fractures. As the acid flows into natural fractures intersecting hydraulic fractures (main fractures), it etches the walls of the natural fracture, which increases the natural fractures' width and generates conductivity. On the other hand, because of the existence of natural fractures, the acid volume in the main fracture reduces, resulting in less conductivity for the main fracture. Existing acid fracturing models estimate the fracture conductivity by assuming the acid flows and reacts in the hydraulic fractures only. In order to accurately predict the performance of acid fracturing in naturally fractured carbonate reservoirs, the acid etching of natural fractures should be taken into account when calculating the overall fracture conductivity.
A model is developed to predict the acid fracturing performance in naturally fractured reservoirs. The model assumes that the main fracture is intersected by transverse symmetric natural fractures. The model simulates the acid transport, acid-rock reaction, fracture width increase due to etching of fracture walls, and acid leakoff through natural fractures. The model also assumes that the flow into natural fractures and the leakoff are pressure dependent and are changing with time. The conductivity calculation is based on the previously developed correlation that takes into account the small scale rock heterogeneities.
The conductivity of natural fractures was found to be a strong function of the leakoff Peclet number, which depends on the velocity of acid flow into natural fractures from an intersecting hydraulic fracture. The effect of natural fractures' geometry on leakoff Peclet number and created fracture conductivity was investigated. The results show that both length and dynamic width, as well as the number of natural fractures, play a significant role in defining the leakoff rate and the conductivity of the hydraulic fracture and the natural fractures. It was also found that the position of the natural fractures along the hydraulic fracture length affects the etching of the natural fractures and the resultant conductivity.
The model will enable the prediction of acid fracture conductivity for naturally fractured reservoirs and improve the feasibility of acid fracturing applications for these type of formations.