bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crystal structure of methyl 8-hydroxy-3-isopropyl-5a,8-dimethyl-2,3,4,5,5a,6,7,8,10a,10b-decahydrocyclohepta[e]indene-3a(1H)-carboxylate, C21H34O3
Authors: Brito Iván , Bórquez Jorge , Simirgiotis Mario , Cárdenas Alejandro , Molina-Salinas Gloria M. , Jerz Gerold , Peña-Rodriguez Luis Manuel , Winterhalter Peter 
Published: February 2016

Abstract
C21H34O3, orthorhombic, P212121 (no. 19), a = 7.5420(7) Å, b = 11.6929(11) Å, c = 22.657(2) Å, V = 1998.1(3) Å3, Z = 4, R
gt
(F) = 0.0383, wR
ref
(F
2
) = 0.113, T = 293(2) K.
Changing exposures in a changing world: models for reducing the burden of disease
Authors: Suk William A. , Mishamandani Sara 
Published: February 2016

Abstract
Environmental exposures are changing dramatically in location, intensity, and frequency. Many developing countries are undergoing a transition in which they face the double burden of infectious diseases as well as chronic diseases. Noncommunicable diseases have emerged as the leading cause of death and disability in developing countries. Globally, pollution is insufficiently appreciated and inadequately quantified as a cause of disease. The health burden from both noninfectious diseases and infectious disease, especially parasites, is high among exposed people. Mothers and children are particularly vulnerable to pollution-related diseases in developing countries. Exposures to pollution can cause protracted noncommunicable diseases across their life span. A global initiative to promote human health sciences and technologies would enhance collaborations and communications amongst investigators and public environmental health officials. Existing models that facilitate the transfer of information and research results exist and can provide insight into building such an international network, allowing better prediction of disease risk and provide ways to reduce exposure to environmental contaminants. A global network would bring together scientists from multiple disciplines and countries to work toward a better understanding of the double burden of disease, especially in low and middle income countries, and promote ways to improve public health.
Semidiscrete approximations for the stationary radiative–conductive heat transfer problem in a two-dimensional system of plates
Authors: Amosov Andrey A. , Maslov Dmitry A. 
Published: February 2016

AbstractSpecial semidiscrete approximations, namely, basic, first, and second semidiscrete problems are proposed for a boundary value problem describing a stationary radiative–conductive heat transfer in a two-dimensional system of heat-conductive plates of width
Discontinuous Galerkin method for wave propagation in elastic media with inhomogeneous inclusions
Authors: Voroshchuk Denis N. , Miryaha Vladislav A. , Petrov Igor B. , Sannikov Alexander V. 
Published: February 2016

AbstractA discontinuous Galerkin method on unstructured grids is adapted and implemented for simulation of wave response of subvertical fractured systems in carbonate rocks for numerical solution of direct problems of seismic exploration. The present paper compares seismic responses for several mechanical-mathematical models of a fractured reservoir. The models of collectors differ in the presence and location of media interfaces relative to the collector and also in a way of its specification, namely, an explicit selection of fractures with the parameters of the medium in the domain of collector coinciding with the host medium, or differing from it. We indicate the ability to take into account inter-fracture interactions with the use of the model of a fractured layer presented in the paper and study wave processes formed as the result of interaction of seismic pulses with fractured reservoirs.
Conceptualizing Stakeholders’ Perceptions of Ecosystem Services: A Participatory Systems Mapping Approach
Authors: Lopes Rita , Videira Nuno 
Published: February 2016

Abstract
A participatory system dynamics modelling approach is advanced to support conceptualization of feedback processes underlying ecosystem services and to foster a shared understanding of leverage intervention points. The process includes systems mapping workshop and follow-up tasks aiming at the collaborative construction of causal loop diagrams. A case study developed in a natural area in Portugal illustrates how a stakeholder group was actively engaged in the development of a conceptual model depicting policies for sustaining the climate regulation ecosystem service.
HMM-DM: identifying differentially methylated regions using a hidden Markov model
Authors: Yu Xiaoqing , Sun Shuying 
Published: February 2016

AbstractDNA methylation is an epigenetic modification involved in organism development and cellular differentiation. Identifying differential methylations can help to study genomic regions associated with diseases. Differential methylation studies on single-CG resolution have become possible with the bisulfite sequencing (BS) technology. However, there is still a lack of efficient statistical methods for identifying differentially methylated (DM) regions in BS data. We have developed a new approach named HMM-DM to detect DM regions between two biological conditions using BS data. This new approach first uses a hidden Markov model (HMM) to identify DM CG sites accounting for spatial correlation across CG sites and variation across samples, and then summarizes identified sites into regions. We demonstrate through a simulation study that our approach has a superior performance compared to BSmooth. We also illustrate the application of HMM-DM using a real breast cancer dataset.
Reliability Analysis of Bearing Capacity of Square Footing on Soil with Strength Anisotropy Due to Layered Microstructure
Authors: Kawa Marek 
Published: February 2016

Abstract
The paper deals with reliability analysis of square footing on soil with strength anisotropy. The strength of the soil has been described with identified anisotropic strength criterion dedicated to geomaterials with layered microstructure. The analysis assumes dip angle α and azimuth angle β which define direction of lamination of the structure to be random variables with given probability density functions. Bearing capacity being a function of these variables is approximated based on results of deterministic simulations obtained for variety of orientations. The weighted regression method by Kaymaz and McMahon within the framework of Response Surface Method is used for the approximation. As a result of analysis, global factor of safety that corresponds to assumed value of probability of failure is determined. The value of the safety factor denotes the ratio between the value of the design load and the mean value of bearing capacity which is needed to reduce the probability of failure to the acceptable level. The procedure of calculating the factor has been presented for two different cases. In the first case, no information about lamination direction of the soil has been provided and thus all the orientations are assumed to be equally probable (uniform distribution). In the second case, statistical information including mean, variance and assumed probability distribution for both α and β angle is known. For the latter case, using results obtained for few different values of mean of angle α, also the influence of strength anisotropy on the value of global factor of safety is shown.
Analysis of the Foundation Slab Settlement of the “Africa Pavilion” Facility in Wrocław
Authors: Sawicki Eugeniusz , Strzelecki Tomasz 
Published: February 2016

Abstract
This article presents the results of numerical calculations of soil consolidation underneath the “Africa Pavilion” structure in Wrocław Zoo, Poland. To determine the deformations of the baseplate of the “Africa Pavilion” and deformations of the subsoil, Biot’s consolidation theory for two-phase medium was applied. The calculations were carried out using the professional program FlexPDE v.6, which is based on the Finite Element Method. Numerical calculations performed were used to evaluate the design assumptions allowing for the laying of hydraulic conduits under the slab.
Comparison Between Two Methods for Estimating the Vertical Scale of Fluctuation for Modeling Random Geotechnical Problems
Authors: Pieczyńska-Kozłowska Joanna M. 
Published: February 2016

Abstract
The design process in geotechnical engineering requires the most accurate mapping of soil. The difficulty lies in the spatial variability of soil parameters, which has been a site of investigation of many researches for many years. This study analyses the soil-modeling problem by suggesting two effective methods of acquiring information for modeling that consists of variability from cone penetration test (CPT). The first method has been used in geotechnical engineering, but the second one has not been associated with geotechnics so far. Both methods are applied to a case study in which the parameters of changes are estimated. The knowledge of the variability of parameters allows in a long term more effective estimation, for example, bearing capacity probability of failure.
Influence of legume crops on content of organic carbon in sandy soil
Authors: Hajduk Edmund , Właśniewski Stanisław , Szpunar-Krok Ewa 
Published: February 2016

AbstractThe paper presents the results of a 3-year field experiment designed to evaluate the content of organic carbon in brown soil (Haplic Cambisol Dystric) developed from a light loamy sand under legumes cultivation. Experimental factors were: species of legume crop (colorful-blooming pea(Pisum sativum), chickling vetch(Lathyrus sativus), narrow-leafed lupin(Lupinus angustifolius), methods of legumes tillage (legumes in pure culture and in mixture with naked oats) and mineral N fertilization (0, 30, 60, 90 kg N·ha−1). Cultivation of legumes on sandy soil did not result in an increase of organic carbon content in the soil after harvest as compared to the initial situation, i.e. 7.39 vs. 7.76 g·kg−1dry matter (DM), on average, respectively. However, there was the beneficial effect of this group of plants on soil abundance in organic matter, the manifestation of which was higher content of organic carbon in soils after legume harvest as compared to soils with oats grown (7.21 g·kg−1DM, on average). Among experimental crops, cultivation of pea exerted the most positive action to organic carbon content (7.58 g·kg−1, after harvest, on average), whereas narrow-leaved lupin had the least effect on organic carbon content (7.23 g·kg−1, on average). Pure culture and greater intensity of legume cultivation associated with the use of higher doses of mineral nitrogen caused less reduction in organic carbon content in soils after harvest.
Selected physical and water properties of soils located in the vicinity of proposed opencast lignite mine “Drzewce” (middle Poland)
Authors: Gajewski Piotr , Kaczmarek Zbigniew , Owczarzak Wojciech , Mocek Andrzej , Glina Bartłomiej 
Published: February 2016

Abstract
The paper presents physical and water properties of six soils located in the areas directly adjacent to “Drzewce” lignite open cast mine (KWB Konin). The conducted works included preparation of pits of various soil types in points characteristic to large and representative soil allotments. The selected soil types represented mineral and organic soils. Samples of disturbed and undisturbed structure were taken from various genetic horizons for laboratory analysis. Such properties as content of total carbon, texture, specific density, bulk density, total and drainage porosity, moisture, saturated hydraulic conductivity, the potential of water bonding, total and readily available water, and total retention were determined in the samples from the horizons of 0–100 cm. The investigated soils showed mostly sandy texture with few local loam insertions. Texture and the content of organic matter were the most important parameters which influenced all analyzed properties. Morphology and the properties of the examined soils as well as deep level of soil – ground water were decisive factors when categorizing them as a precipitation-water type. Such soils will not undergo degradation caused by the dehydrating depression cone of open cast mine.
Importance of Unit Cells in Accurate Evaluation of the Characteristics of Graphene
Authors: Sabzyan Hassan , Sadeghpour Narges 
Published: February 2016

Abstract
Effects of the size of the unit cell on energy, atomic charges, and phonon frequencies of graphene at the Γ point of the Brillouin zone are studied in the absence and presence of an electric field using density functional theory (DFT) methods (LDA and DFT-PBE functionals with Goedecker–Teter–Hutter (GTH) and Troullier–Martins (TM) norm-conserving pseudopotentials). Two types of unit cells containing nC=4–28 carbon atoms are considered. Results show that stability of graphene increases with increasing size of the unit cell. Energy, atomic charges, and phonon frequencies all converge above nC=24 for all functional-pseudopotentials used. Except for the LDA-GTH calculations, application of an electric field of 0.4 and 0.9 V/nm strengths does not change the trends with the size of the unit cell but instead slightly decreases the binding energy of graphene. Results of this study show that the choice of unit cell size and type is critical for calculation of reliable characteristics of graphene.
Tau-Driven Neuronal and Neurotrophic Dysfunction in a Mouse Model of Early Tauopathy
Authors: Mazzaro Nadia , Barini Erica , Spillantini Maria Grazia , Goedert Michel , Medini Paolo , Gasparini Laura 
Published: February 2016

Tauopathies are neurodegenerative diseases characterized by intraneuronal inclusions of hyperphosphorylated tau protein and abnormal expression of brain-derived neurotrophic factor (BDNF), a key modulator of neuronal survival and function. The severity of both these pathological hallmarks correlate with the degree of cognitive impairment in patients. However, how tau pathology specifically modifies BDNF signaling and affects neuronal function during early prodromal stages of tauopathy remains unclear. Here, we report that the mild tauopathy developing in retinal ganglion cells (RGCs) of the P301S tau transgenic (P301S) mouse induces functional retinal changes by disrupting BDNF signaling via the TrkB receptor. In adult P301S mice, the physiological visual response of RGCs to pattern light stimuli and retinal acuity decline significantly. As a consequence, the activity-dependent secretion of BDNF in the vitreous is impaired in P301S mice. Further, in P301S retinas, TrkB receptors are selectively upregulated, but uncoupled from downstream extracellular signal-regulated kinase (ERK) 1/2 signaling. We also show that the impairment of TrkB signaling is triggered by tau pathology and mediates the tau-induced dysfunction of visual response. Overall our results identify a neurotrophin-mediated mechanism by which tau induces neuronal dysfunction during prodromal stages of tauopathy and define tau-driven pathophysiological changes of potential value to support early diagnosis and informed therapeutic decisions.SIGNIFICANCE STATEMENTThis work highlights the potential molecular mechanisms by which initial tauopathy induces neuronal dysfunction. Combining clinically used electrophysiological techniques (i.e., electroretinography) and molecular analyses, this work shows that in a relevant model of early tauopathy, the retina of the P301S mutant human tau transgenic mouse, mild tau pathology results in functional changes of neuronal activity, likely due to selective impairment of brain-derived neurotrophic factor signaling via its receptor, TrkB. These findings may have important translational implications for early diagnosis in a subset of Alzheimer's disease patients with early visual symptoms and emphasize the need to clarify the pathophysiological changes associated with distinct tauopathy stages to support informed therapeutic decisions and guide drug discovery.
Impaired Dendritic Development and Memory inSorbs2Knock-Out Mice
Authors: Zhang Qiangge , Gao Xian , Li Chenchen , Feliciano Catia , Wang Dongqing , Zhou Dingxi , Mei Yuan , Monteiro Patricia , Anand Michelle , Itohara Shigeyoshi , Dong Xiaowei , Fu Zhanyan , Feng Guoping 
Published: February 2016

Intellectual disability is a common neurodevelopmental disorder characterized by impaired intellectual and adaptive functioning. Both environmental insults and genetic defects contribute to the etiology of intellectual disability. Copy number variations ofSORBS2have been linked to intellectual disability. However, the neurobiological function ofSORBS2in the brain is unknown. TheSORBS2gene encodes ArgBP2 (Arg/c-Abl kinase binding protein 2) protein in non-neuronal tissues and is alternatively spliced in the brain to encode nArgBP2 protein. We found nArgBP2 colocalized with F-actin at dendritic spines and growth cones in cultured hippocampal neurons. In the mouse brain, nArgBP2 was highly expressed in the cortex, amygdala, and hippocampus, and enriched in the outer one-third of the molecular layer in dentate gyrus. Genetic deletion ofSorbs2in mice led to reduced dendritic complexity and decreased frequency of AMPAR-miniature spontaneous EPSCs in dentate gyrus granule cells. Behavioral characterization revealed thatSorbs2deletion led to a reduced acoustic startle response, and defective long-term object recognition memory and contextual fear memory. Together, our findings demonstrate, for the first time, an important role for nArgBP2 in neuronal dendritic development and excitatory synaptic transmission, which may thus inform exploration of neurobiological basis ofSORBS2deficiency in intellectual disability.SIGNIFICANCE STATEMENTCopy number variations of theSORBS2gene are linked to intellectual disability, but the neurobiological mechanisms are unknown. We found that nArgBP2, the only neuronal isoform encoded bySORBS2, colocalizes with F-actin at neuronal dendritic growth cones and spines. nArgBP2 is highly expressed in the cortex, amygdala, and dentate gyrus in the mouse brain. Genetic deletion ofSorbs2in mice leads to impaired dendritic complexity and reduced excitatory synaptic transmission in dentate gyrus granule cells, accompanied by behavioral deficits in acoustic startle response and long-term memory. This is the first study ofSorbs2function in the brain, and our findings may facilitate the study of neurobiological mechanisms underlyingSORBS2deficiency in the development of intellectual disability.
Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed
Authors: Benned-Jensen Tau , Christensen Rasmus Kordt , Denti Federico , Perrier Jean-Francois , Rasmussen Hanne Borger , Olesen Søren-Peter 
Published: February 2016

The voltage-gated K+channels Kv7.2 and Kv7.3 are located at the axon initial segment (AIS) and exert strong control over action potential generation. Therefore, changes in their localization or cell surface numbers are likely to influence neuronal signaling. However, nothing is known about the cell surface dynamics of Kv7.2/7.3 at steady state or during short-term neuronal stimulation. This is primarily attributable to their membrane topology, which hampers extracellular epitope tagging. Here we circumvent this limitation by fusing an extra phluorin-tagged helix to the N terminus of human Kv7.3. This seven transmembrane chimera, named super ecliptic phluorin (SEP)–TAC-7.3, functions and traffics as a wild-type (WT) channel. We expressed SEP–TAC-7.3 in dissociated rat hippocampal neurons to examine the lateral mobility, surface numbers, and localization of AIS Kv7.2/7.3 heteromers using live imaging. We discovered that they are extraordinarily stable and exhibit a very low surface mobility both during steady state and neuronal stimulation. In the latter case, we also found that neither localization nor cell surface numbers were changed. However, at high glutamate loads, we observed a rapid irreversible endocytosis of Kv7.2/7.3, which required the activation of NR2B-containing NMDA receptors, Ca2+influx, and calpain activation. This excitotoxic mechanism may be specific to ankyrin G-bound AIS proteins because Nav1.2 channels, but not AIS GABAAreceptors, were also endocytosed. In conclusion, we have, for the first time, characterized the cell surface dynamics of a full-length Kv7 channel using a novel chimeric strategy. This approach is likely also applicable to other Kv channels and thus of value for the additional characterization of this ion channel subfamily.SIGNIFICANCE STATEMENTThe voltage-gated K+channels Kv7.2 and Kv7.3 exert strong control over action potential generation, but little is known about their cell surface dynamics. Using a novel phluorin-based approach, we here show that these channels are highly stable at steady state and different types of neuronal stimulation. However, at high glutamate loads, they undergo a rapid calpain-dependent endocytosis that likely represents an early response during excitotoxic states.
Perceptual Learning at a Conceptual Level
Authors: Wang Rui , Wang Jie , Zhang Jun-Yun , Xie Xin-Yu , Yang Yu-Xiang , Luo Shu-Han , Yu Cong , Li Wu 
Published: February 2016

Humans can learn to abstract and conceptualize the shared visual features defining an object category in object learning. Therefore, learning is generalizable to transformations of familiar objects and even to new objects that differ in other physical properties. In contrast, visual perceptual learning (VPL), improvement in discriminating fine differences of a basic visual feature through training, is commonly regarded as specific and low-level learning because the improvement often disappears when the trained stimulus is simply relocated or rotated in the visual field. Such location and orientation specificity is taken as evidence for neural plasticity in primary visual cortex (V1) or improved readout of V1 signals. However, new training methods have shown complete VPL transfer across stimulus locations and orientations, suggesting the involvement of high-level cognitive processes. Here we report that VPL bears similar properties of object learning. Specifically, we found that orientation discrimination learning is completely transferrable between luminance gratings initially encoded in V1 and bilaterally symmetric dot patterns encoded in higher visual cortex. Similarly, motion direction discrimination learning is transferable between first- and second-order motion signals. These results suggest that VPL can take place at a conceptual level and generalize to stimuli with different physical properties. Our findings thus reconcile perceptual and object learning into a unified framework.SIGNIFICANCE STATEMENTTraining in object recognition can produce a learning effect that is applicable to new viewing conditions or even to new objects with different physical properties. However, perceptual learning has long been regarded as a low-level form of learning because of its specificity to the trained stimulus conditions. Here we demonstrate with new training tactics that visual perceptual learning is completely transferrable between distinct physical stimuli. This finding indicates that perceptual learning also operates at a conceptual level in a stimulus-invariant manner.