bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21
Authors: Wang Kan , Jiang Zhi , Webster Keith A. , Chen Jinghai , Hu Hengxun , Zhou Yu , Zhao Jing , Wang Lihan , Wang Yingchao , Zhong Zhiwei , Ni Cheng , Li Qingju , Xiang Charlie , Zhang Ling , Wu Rongrong , Zhu Wei , Yu Hong , Hu Xinyang , Wang Jian'an 
Published: August 2016

Abstract
Our group recently reported positive therapeutic benefit of human endometrium-derived mesenchymal stem cells (EnMSCs) delivered to infarcted rat myocardium, an effect that correlated with enhanced secretion of protective cytokines and growth factors compared with parallel cultures of human bone marrow MSCs (BMMSCs). To define more precisely the molecular mechanisms of EnMSC therapy, in the present study, we assessed in parallel the paracrine and therapeutic properties of MSCs derived from endometrium, bone marrow, and adipose tissues in a rat model of myocardial infarction (MI). EnMSCs, BMMSCs, and adipose-derived MSCs (AdMSCs) were characterized by fluorescence-activated cell sorting (FACS). Paracrine and cytoprotective actions were assessed in vitro by coculture with neonatal cardiomyocytes and human umbilical vein endothelial cells. A rat MI model was used to compare cell therapy by intramyocardial injection of BMMSCs, AdMSCs, and EnMSCs. We found that EnMSCs conferred superior cardioprotection relative to BMMSCs or AdMSCs and supported enhanced microvessel density. Inhibitor studies indicated that the enhanced paracrine actions of EnMSCs were mediated by secreted exosomes. Analyses of exosomal microRNAs (miRs) by miR array and quantitative polymerase chain reaction revealed that miR-21 expression was selectively enhanced in exosomes derived from EnMSCs. Selective antagonism of miR-21 by anti-miR treatment abolished the antiapoptotic and angiogenic effects of EnMSCs with parallel effects on phosphatase and tensin homolog (PTEN), a miR-21 target and downstream Akt. The results of the present study confirm the superior cardioprotection by EnMSCs relative to BMMSCs or AdMSCs and implicates miR-21 as a potential mediator of EnMSC therapy by enhancing cell survival through the PTEN/Akt pathway. The endometrium might be a preferential source of MSCs for cardiovascular cell therapy.
Ferumoxytol Labeling of Human Neural Progenitor Cells for Diagnostic Cellular Tracking in the Porcine Spinal Cord with Magnetic Resonance Imaging
Authors: Lamanna Jason J. , Gutierrez Juanmarco , Urquia Lindsey N. , Hurtig C. Victor , Amador Elman , Grin Natalia , Svendsen Clive N. , Federici Thais , Oshinski John N. , Boulis Nicholas M. 
Published: August 2016

Abstract
We report on the diagnostic capability of magnetic resonance imaging (MRI)-based tracking of ferumoxytol-labeled human neural progenitor cells (hNPCs) transplanted into the porcine spinal cord. hNPCs prelabeled with two doses of ferumoxytol nanoparticles (hNPC-FLow and hNPC-FHigh) were injected into the ventral horn of the spinal cord in healthy minipigs. Ferumoxytol-labeled grafts were tracked in vivo up to 105 days after transplantation with MRI. Injection accuracy was assessed in vivo at day 14 and was predictive of “on” or “off” target cell graft location assessed by histology. No difference in long-term cell survival, assessed by quantitative stereology, was observed among hNPC-FLow, hNPC-FHigh, or control grafts. Histological iron colocalized with MRI signal and engrafted human nuclei. Furthermore, the ferumoxytol-labeled cells retained nanoparticles and function in vivo. This approach represents an important leap forward toward facilitating translation of cell-tracking technologies to clinical trials by providing a method of assessing transplantation accuracy, delivered dose, and potentially cell survival.
Comparative Clinical Observation of Arthroscopic Microfracture in the Presence and Absence of a Stromal Vascular Fraction Injection for Osteoarthritis
Authors: Nguyen Phu Dinh , Tran Tung Dang-Xuan , Nguyen Huynh Ton-Ngoc , Vu Hieu Trung , Le Phuong Thi-Bich , Phan Nhan Lu-Chinh , Vu Ngoc Bich , Phan Ngoc Kim , Van Pham Phuc 
Published: August 2016

Abstract
Osteoarthritis (OA) is a degenerative cartilage disease that is characterized by a local inflammatory reaction. Consequently, many studies have been performed to identify suitable prevention and treatment interventions. In recent years, both arthroscopic microfracture (AM) and stem cell therapy have been used clinically to treat OA. This study aimed to evaluate the clinical effects of AM in the presence and absence of a stromal vascular fraction (SVF) injection in the management of patients with OA. Thirty patients with grade 2 or 3 (Lawrence scale) OA of the knee participated in this study. Placebo group patients (n = 15) received AM alone; treatment group patients (n = 15) received AM and an adipose tissue-derived SVF injection. The SVF was suspended in platelet-rich plasma (PRP) before injection into the joint. Patient groups were monitored and scored with the Western Ontario and McMaster Universities Arthritis Index (WOMAC), Lysholm, Visual Analog Pain Scale (VAS), and modified Outerbridge classifications before treatment and at 6, 12, and 18 months post-treatment. Bone marrow edema was also assessed at these time points. Patients were evaluated for knee activity (joint motion amplitude) and adverse effects relating to surgery and stem cell injection. Treatment efficacy was significantly different between placebo and treatment groups. All treatment group patients had significantly reduced pain and WOMAC scores, and increased Lysholm and VAS scores compared with the placebo group. These findings suggest that the SVF/PRP injection efficiently improved OA for 18 months after treatment. This study will be continuously monitored for additional 24 months.
The Influence of Neighborhood Aesthetics, Safety, and Social Cohesion on Perceived Stress in Disadvantaged Communities
Authors: Henderson Heather , Child Stephanie , Moore Spencer , Moore Justin B. , Kaczynski Andrew T. 
Published: August 2016

AbstractLimited research has explored how specific elements of physical and social environments influence mental health indicators such as perceived stress, or whether such associations are moderated by gender. This study examined the relationship between selected neighborhood characteristics and perceived stress levels within a primarily low‐income, older, African‐American population in a mid‐sized city in the Southeastern U.S. Residents (n = 394; mean age=55.3 years, 70.9% female, 89.3% African American) from eight historically disadvantaged neighborhoods completed surveys measuring perceptions of neighborhood safety, social cohesion, aesthetics, and stress. Multivariate linear regression models examined the association between each of the three neighborhood characteristics and perceived stress. Greater perceived safety, improved neighborhood aesthetics, and social cohesion were significantly associated with lower perceived stress. These associations were not moderated by gender. These findings suggest that improving social attributes of neighborhoods may have positive impacts on stress and related benefits for population health. Future research should examine how neighborhood characteristics influence stress over time.
GaCl3‐Mediated Reactions of Donor–Acceptor Cyclopropanes with Aromatic Aldehydes
Authors: Borisov Denis D. , Novikov Roman A. , Tomilov Yury V. 
Published: August 2016

AbstractA new strategy for cascade assembly of substituted indenes and polycyclic lactones based on reactions of donor–acceptor cyclopropanes and styrylmalonates with aromatic aldehydes in the presence of GaCl3 has been developed. The use of GaCl3 makes it possible to principally change the direction of the reaction known in this series of substrates and to perform the process in a multicomponent version. Generation of formal 1,2‐zwitterionic intermediates owing to complexation of dicarboxylate groups with GaCl3 is the driving force of the reactions discovered. This method makes it possible to assemble indenylmalonates or indano[1′,2′:2,3]indano[2,1‐b]furan‐2‐ones in one synthetic stage from readily available starting compounds with high regio‐ and diastereoselectivity. A mechanism of the reactions has been suggested using the 18O label in benzaldehyde.
Evaluierung neuer Reaktionen zur Steuerung der Wirkstoff‐Forschung: ein Eignungstest
Authors: Foley Daniel J. , Nelson Adam , Marsden Stephen P. 
Published: August 2016

AbstractMit der Verbesserung unserer Kenntnisse zur Bedeutung bestimmter Moleküleigenschaften bei Anwendungen im Rahmen der Wirkstoffsuche wird das Ausmaß immer klarer, in dem veröffentlichte Synthesemethoden erwünschten Kriterien genügen (oder nicht genügen). Wir beschreiben hier, wie die Anwendung einfacher (und in vielen Fällen frei verfügbarer) Rechenprogramme genutzt werden kann, um ein semiquantitatives Verständnis des Potenzials von neuen Methoden in der Wirkstoff‐Forschung zu entwickeln. Diese Analyse kann sich unter anderem auf die Planung verbesserter Studien zum Substratspektrum auswirken, die Priorisierung von bestimmten Beispielstrukturen für die Synthese leiten und Ansprüche für potenzielle künftige Anwendungen von neuen Methoden begründen.
Organic‐Stabilizer‐Free Polyol Synthesis of Silver Nanowires for Electrode Applications
Authors: Sim Hwansu , Bok Shingyu , Kim Bongsung , Kim Minha , Lim Guh‐Hwan , Cho Sung Min , Lim Byungkwon 
Published: August 2016

AbstractThe polyol reduction of a Ag precursor in the presence of an organic stabilizer, such as poly(vinylpyrrolidone), is a widely used method for the production of Ag nanowires (NWs). However, organic capping molecules introduce insulating layers around each NW. Herein we demonstrate that Ag NWs can be produced in high yield without any organic stabilizers simply by introducing trace amounts of NaCl and Fe(NO3)3 during low‐temperature polyol synthesis. The heterogeneous nucleation and growth of Ag NWs on initially formed AgCl particles, combined with oxidative etching of unwanted Ag nanoparticles, resulted in the selective formation of long NWs with an average length of about 40 μm in the absence of a capping or stabilizing effect provided by surface‐adsorbing molecules. These organic‐stabilizer‐free Ag NWs were directly used for the fabrication of high‐performance transparent or stretchable electrodes without a complicated process for the removal of capping molecules from the NW surface.
Targeted Delivery and Sustained Antitumor Activity of Triptolide through Glucose Conjugation
Authors: He Qing‐Li , Minn Il , Wang Qiaoling , Xu Peng , Head Sarah A. , Datan Emmanuel , Yu Biao , Pomper Martin G. , Liu Jun O. 
Published: August 2016

AbstractTriptolide, a key ingredient from the traditional Chinese medicinal plant thunder god vine, which has been used to treat inflammation and autoimmune diseases for centuries, has been shown to be an irreversible inhibitor of the XPB subunit of the transcription factor TFIIH and initiation of RNA polymerase II mediated transcription. The clinical development of triptolide over the past two decades has been limited by its toxicity and low water solubility. Herein, we report the development of a glucose conjugate of triptolide, named glutriptolide, which was intended to target tumor cells overexpressing glucose transporters selectively. Glutriptolide did not inhibit XPB activity in vitro but demonstrated significantly higher cytotoxicity against tumor cells over normal cells with greater water solubility than triptolide. Furthermore, it exhibited remarkable tumor control in vivo, which is likely due to sustained stepwise release of active triptolide within cancer cells. These findings indicate that glutriptolide may serve as a promising lead for developing a new mechanistic class of anticancer drugs.
Hochaktive und selektive Mangankatalysatoren zur Hydrierung von C=O‐Bindungen – die Bedeutung des mehrzähnigen Liganden, der Coliganden und der Oxidationsstufe
Authors: Kallmeier Fabian , Irrgang Torsten , Dietel Thomas , Kempe Rhett 
Published: August 2016

AbstractDer Ersatz teurer Edelmetalle durch in der Erde reichlich vorkommende Übergangsmetalle ist ein zentrales Thema in der Katalyse. Hier wird ein hochaktiver und selektiver, homogener, Mn‐basierter Hydrierkatalysator von C=O‐Bindungen vorgestellt. Der Katalysator verfügt über ein breites Substratspektrum und ist in der Lage, Aryl‐alkyl‐, Diaryl‐, Dialkyl‐ und Cycloalkylketone sowie Aldehyde zu hydrieren. Eine sehr gute Verträglichkeit mit funktionellen Gruppen, einschließlich der quantitativen und selektiven Hydrierung eines Ketons in Gegenwart eines nicht abgeschirmten Olefins, wird beobachtet. In der Mn‐Hydrierkatalyse ist die Kombination aus mehrzähnigem Liganden, Oxidationszustand des Metalls und richtigem Hilfsliganden wahrscheinlich entscheidend für eine hohe Aktivität. Diese Beobachtung unterstreicht einen Vorteil von Homogenkatalysatoren in der 3d‐Metallkatalyse. Für Koordinationsverbindungen ist eine Feinabstimmung einer komplexen Koordinationsumgebung leichter durchführbar als für Enzyme und/oder Heterogenkatalysatoren.
Nickel‐Catalyzed Enantioselective Reductive Amination of Ketones with Both Arylamines and Benzhydrazide
Authors: Yang Peng , Lim Li Hui , Chuanprasit Pratanphorn , Hirao Hajime , Zhou Jianrong (Steve) 
Published: August 2016

AbstractAn asymmetric reductive amination of ketones using both arylamines and benzhydrazide in the presence of nickel catalysts was developed. A one‐pot synthesis of tetrahydroquinoxalines was also developed starting directly from α‐ketoaldehydes and 1,2‐diaminobenzene. Formic acid was used as a safe and economic surrogate for high‐pressure hydrogen gas. Strongly σ‐donating bis(alkylphosphine)s are crucial ancillary ligands for both stereoselective hydride insertion and decarboxylation of the formate.
Evaluating New Chemistry to Drive Molecular Discovery: Fit for Purpose?
Authors: Foley Daniel J. , Nelson Adam , Marsden Stephen P. 
Published: August 2016

AbstractAs our understanding of the impact of specific molecular properties on applications in discovery‐based disciplines improves, the extent to which published synthetic methods meet (or do not meet) desirable criteria is ever clearer. Herein, we show how the application of simple (and in many cases freely available) computational tools can be used to develop a semiquantitative understanding of the potential of new methods to support molecular discovery. This analysis can, among other things, inform the design of improved substrate scoping studies; direct the prioritization of specific exemplar structures for synthesis; and substantiate claims of potential future applications for new methods.
Highly Active and Selective Manganese C=O Bond Hydrogenation Catalysts: The Importance of the Multidentate Ligand, the Ancillary Ligands, and the Oxidation State
Authors: Kallmeier Fabian , Irrgang Torsten , Dietel Thomas , Kempe Rhett 
Published: August 2016

AbstractThe replacement of expensive noble metals by earth‐abundant transition metals is a central topic in catalysis. Herein, we introduce a highly active and selective homogeneous manganese‐based C=O bond hydrogenation catalyst. Our catalyst has a broad substrate scope, it is able to hydrogenate aryl–alkyl, diaryl, dialkyl, and cycloalkyl ketones as well as aldehydes. A very good functional group tolerance including the quantitative and selective hydrogenation of a ketone in the presence of a non‐shielded olefin is observed. In Mn hydrogenation catalysis, the combination of the multidentate ligand, the oxidation state of the metal, and the choice of the right ancillary ligand is crucial for high activity. This observation emphasizes an advantage and the importance of homogeneous catalysts in 3d‐metal catalysis. For coordination compounds, fine‐tuning of a complex coordination environment is easily accomplished in comparison to enzyme and/or heterogeneous catalysts.
Influence of rubber content on mechanical, thermal, and morphological behavior of natural rubber toughened poly(lactic acid)–multiwalled carbon nanotube nanocomposites
Authors: Mat Desa Mohd Shaiful Zaidi , Hassan Azman , Arsad Agus , Arjmandi Reza , Mohammad Nor Nisa Balqis 
Published: August 2016

ABSTRACTThe effects of natural rubber (NR) on the mechanical, thermal, and morphological properties of multiwalled carbon nanotube (CNT) reinforced poly(lactic acid) (PLA) nanocomposites prepared by melt blending were investigated. A PLA/NR blend and PLA/CNT nanocomposites were also produced for comparison. The tensile strength and Young's modulus of PLA/CNT nanocomposites improved significantly, whereas the impact strength decreased compared to neat PLA. The incorporation of NR into PLA/CNT significantly improved the impact strength and elongation at break of the nanocomposites, which showed approximately 200% and 850% increases at 20 wt % NR, respectively. However, the tensile strength and Young's modulus of PLA/NR/CNT nanocomposites decreased compared to PLA/CNT nanocomposites. The morphology analysis showed the homogeneous dispersion of NR particles in PLA/NR/CNT nanocomposites, while CNTs preferentially reside in the NR phase rather than the PLA matrix. In addition, the incorporation of NR into PLA/CNT lowered the thermal stability and glass‐transition temperature of the nanocomposites. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44344.
Surface modification of microcrystalline cellulose (MCC) and its application in LDPE‐based composites
Authors: Chartrand Ariane , Lavoie Jean‐Michel , Huneault Michel A. 
Published: August 2016

ABSTRACTMicrocrystalline cellulose (MCC) was modified by grafting onto its surface ferulic acid, methacryloyl chloride and oleoyl chloride. The efficacy of the chemical modification was confirmed by X‐ray photoelectron spectroscopy. In addition, the size distribution of the cellulosic particles was investigated by optical microscopy and laser granulometry and its hydrophobicity was evaluated using a contact angle method. Finally, to investigate the affinity of modified MCC with a nonpolar polymer and to assess its potential as a biobased reinforcing filler, the modified MCC was compounded into low‐density polyethylene. An organic peroxide, dicumyl peroxide, was added at selected formulations to see if it could further enhance mechanical bonding between the polymer and the particulates. The dispersion was assessed by scanning electron microscopy. Mechanical properties were investigated through tensile testing while the melt rheology of the composites was monitored by small angle oscillatory shear rheology. The acylation modification of the MCC improved the dispersion within LDPE and enhanced the mechanical properties of the composites. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44348.
Chemoselective, Substrate‐directed Fluorination of Functionalized Cyclopentane β‐Amino Acids
Authors: Kiss Loránd , Nonn Melinda , Sillanpää Reijo , Haukka Matti , Fustero Santos , Fülöp Ferenc 
Published: August 2016

AbstractThis work describes a substrate‐directed fluorination of some highly functionalized cyclopentane derivatives. The cyclic products incorporating CH2F or CHF2 moieties in their structure have been synthesized from diexo‐ or diendo‐norbornene β‐amino acids following a stereocontrolled strategy. The synthetic study was based on an oxidative transformation of the ring carbon–carbon double bond of the norbornene β‐amino acids, followed by transformation of the resulted „all cis“ and „trans“ diformyl intermediates by fluorination with „chemodifferentiation“.
Enhanced noradrenergic axon regeneration into schwann cell‐filled PVDF‐TrFE conduits after complete spinal cord transection
Authors: Lee Yee‐Shuan , Wu Siliang , Arinzeh Treena Livingston , Bunge Mary Bartlett 
Published: August 2016

ABSTRACTSchwann cell (SC) transplantation has been utilized for spinal cord repair and demonstrated to be a promising therapeutic strategy. In this study, we investigated the feasibility of combining SC transplantation with novel conduits to bridge the completely transected adult rat spinal cord. This is the first and initial study to evaluate the potential of using a fibrous piezoelectric polyvinylidene fluoride trifluoroethylene (PVDF‐TrFE) conduit with SCs for spinal cord repair. PVDF‐TrFE has been shown to enhance neurite growth in vitro and peripheral nerve repair in vivo. In this study, SCs adhered and proliferated when seeded onto PVDF‐TrFE scaffolds in vitro. SCs and PVDF‐TrFE conduits, consisting of random or aligned fibrous inner walls, were transplanted into transected rat spinal cords for 3 weeks to examine early repair. Glial fibrillary acidic protein (GFAP)+astrocyte processes and GFP (green fluorescent protein)‐SCs were interdigitated at both rostral and caudal spinal cord/SC transplant interfaces in both types of conduits, indicative of permissivity to axon growth. More noradrenergic/DβH+(dopamine‐beta‐hydroxylase) brainstem axons regenerated across the transplant when greater numbers of GFAP+astrocyte processes were present. Aligned conduits promoted extension of DβH+axons and GFAP+processes farther into the transplant than random conduits. Sensory CGRP+(calcitonin gene‐related peptide) axons were present at the caudal interface. Blood vessels formed throughout the transplant in both conduits. This study demonstrates that PVDF‐TrFE conduits harboring SCs are promising for spinal cord repair and deserve further investigation. Biotechnol. Bioeng. 2017;114: 444–456. © 2016 Wiley Periodicals, Inc.
Determination of eurycomanone in rat plasma using hydrophilic interaction liquid chromatography–tandem mass spectrometry for pharmacokinetic study
Authors: Rehman Shaheed Ur , Choi Min Sun , Han Young Min , Kim In Sook , Kim Seung Hyun , Piao Xiang‐Lan , Yoo Hye Hyun 
Published: August 2016

AbstractIn this study, a rapid, sensitive, and reliable hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC‐MS/MS) method for the determination of eurycomanone in rat plasma was developed and validated. Plasma samples were pretreated with a protein precipitation method and quercitrin was used as an internal standard (IS). A HILIC silica column (2.1 × 100 mm, 3 μm) was used for hydrophilic‐based chromatographic separation, using the mobile phase of 0.1% formic acid with acetonitrile in gradient elution at a flow rate of 0.25 mL/min. Precursor–product ion pairs for multiple‐reaction monitoring were m/z 409.1 → 391.0 for eurycomanone and m/z 449.1 → 303.0 for IS. The linear range was 2–120 ng/mL. The intra‐ and inter‐day accuracies were between 95.5 and 103.4% with a precision of <4.2%. The developed method was successfully applied to the pharmacokinetic analysis of eurycomanone in rat plasma after oral dosing with pure compound and E. longifolia extract. The Cmax and AUC0–t, respectively, were 40.43 ± 16.08 ng/mL and 161.09 ± 37.63 ng h/mL for 10 mg/kg eurycomanone, and 9.90 ± 3.97 ng/mL and 37.15 ± 6.80 ng h/mL for E. longifolia extract (2 mg/kg as eurycomanone). The pharmacokinetic results were comparable with each other, based on the dose as eurycomanone.