bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Analysis of the Safety and Feasibility of Laparoscopic Versus Open Segment 7 Hepatectomy
Authors: Zeng Haitao , Xiong Xiaoli , Huang Song , Zhang Jia , Liu Hongliang , Huang Yong 
Published: October 2024

Background:
Laparoscopic hepatectomy has been widely accepted owing to its advantages as a minimally invasive surgery; however, laparoscopic segment 7 (S7) hepatectomy (LSH) has been rarely reported. We aimed to explore the safety and feasibility of LSH by comparing it with open surgical approaches.

Methods:
Twenty-nine patients who underwent S7 hepatectomy between January 2016 and January 2023 were enrolled in this study. The patients’ characteristics, intraoperative details, and postoperative outcomes were compared between the 2 groups.

Results:
No significant differences were observed in the preoperative data. The patients who underwent LSH had significantly shorter hospital stays (P=0.016) but longer operative times (P=0.034) than those who underwent open S7 hepatectomy. No significant differences in blood loss (P=0.614), transfusion (P=0.316), hospital expenses (P=0.391), surgical margin (P=0.442), rate of other complications, postoperative white blood cell count, and alanine aminotransferase and aspartate aminotransferase levels were noted between the 2 groups (P>0.05). For hepatocellular carcinoma, the results showed no differences in either disease-free survival (P=0.432) or overall survival (P=0.923) between the 2 groups.

Conclusions:
LSH is a safe and feasible surgical procedure that is efficient from an oncological point of view. It may be the preferred technique for lesions in the S7 of the liver.
Minimally Invasive Pauli Parastomal Hernia Repair
Authors: Guerra Francesco , Giuliani Giuseppe , Salvischiani Lucia , Genovese Alfredo , Coratti Andrea 
Published: October 2024

Background:
Although originally described as an open procedure, the application in the setting of minimally invasive surgery of the Pauli technique for parastomal hernia repair is gaining interest among surgeons owing to encouraging early results. We aimed at combining and analyzing the results of minimally invasive Pauli repair by assessing the available evidence.

Methods:
A literature search in Pubmed, Embase, and Web of Science was undertaken to include all articles reporting on minimally invasive repair of parastomal hernias using the Pauli technique.

Results:
Data regarding a total of 75 patients across 11 articles published between 2019 and 2023 were included. Patients received surgery either by a laparoscopic (27%) or robotic (73%) approach. A transabdominal route was chosen in 62 patients (83%), while an extraperitoneal technique was employed in the remaining 13 patients. The rate of postoperative morbidity was 34%, with 10% being the incidence of grade >II complications. The reported overall rate of recurrence was 7% at a median follow-up of 1 to 43 months.

Conclusions:
The available evidence derived from a growing number of centers suggests that minimally invasive Pauli repair is a viable option to treat parastomal hernias. Despite robust, high-level data still lacking, preliminary experiences indicate promising results.
Transplantation: A Priority in the Healthcare Agenda
Authors: Muller Elmi , Dominguez-Gil Beatriz , Ahn Curie , Berenguer Marina , Cardillo Massimo , Chatzixiros Efstratios , Cortesi Paolo , Herson Marisa , Ilbawi Andre , Jha Vivekanand , Mahillo Beatriz , Manas Derek M. , Nino-Murcia Alejandro , Shaheen Faissal A.M. , Stock Peter , Potena Luciano 
Published: October 2024

Background.
In November 2023, in the context of the Spanish Presidency of the Council of the European Union, the Organization National de Transplante organized a global summit discussing global action in transplantation for the next decade. This article reports the recommendations supporting the need to prioritize transplantation in healthcare systems.

Methods.
The working group investigated how transplantation addresses noncommunicable disease mortality, particularly related to kidney and liver disease. They also investigated how transplantation can contribute to the achievement of several of the United Nations Sustainable Development Goals, especially Goal 3 (good health and well-being), Goal 8 (sustained, inclusive, and sustainable economic growth and employment for all), and Goal 13 (combat climate change and its impact).

Results.
By prioritizing transplantation, the increased availability and accessibility of life-saving organs and tissues to the public will not only lead to saving more lives and improving health outcomes for individual patients but also contribute to the development of a resilient health system in general in that country as a consequence of developing the infrastructure required for transplantation.

Conclusions.
The ethical principles associated with transplantation promote the principles of solidarity in society by fostering the donation process and equity in access to therapy. This article aims to advocate for the widespread availability of solid organ, tissue, and cell transplantation for all patients.
Behavioural responses of common dolphins to naval sonar
Authors: Southall Brandon L. , Durban John W. , Calambokidis John , Casey Caroline , Fahlbusch James A. , Fearnbach Holly , Flynn Kiirsten R. , Fregosi Selene , Friedlaender Ari S. , Leander Samantha G. M. , Visser Fleur 
Published: October 2024

Despite strong interest in how noise affects marine mammals, little is known for the most abundant and commonly exposed taxa. Social delphinids occur in groups of hundreds of individuals that travel quickly, change behaviour ephemerally and are not amenable to conventional tagging methods, posing challenges in quantifying noise impacts. We integrated drone-based photogrammetry, strategically placed acoustic recorders and broad-scale visual observations to provide complementary measurements of different aspects of behaviour for short- and long-beaked common dolphins. We measured behavioural responses during controlled exposure experiments (CEEs) of military mid-frequency (3–4 kHz) active sonar (MFAS) using simulated and actual Navy sonar sources. We used latent-state Bayesian models to evaluate response probability and persistence in exposure and post-exposure phases. Changes in subgroup movement and aggregation parameters were commonly detected during different phases of MFAS CEEs but not control CEEs. Responses were more evident in short-beaked common dolphins (
n
= 14 CEEs), and a direct relationship between response probability and received level was observed. Long-beaked common dolphins (
n
= 20) showed less consistent responses, although contextual differences may have limited which movement responses could be detected. These are the first experimental behavioural response data for these abundant dolphins to directly inform impact assessments for military sonars.
Compatibility and comparative analysis of chronological and biological aging between the legacy 450K and the EPIC v2.0 arrays
Authors: De Pourcq Sven , Kuo Pei-Lun , Moore Ann Zenobia , Bandinelli Stefania , Horvath Steve , Ferrucci Luigi , Santini Valeria 
Published: October 2024

AbstractSeveral different epigenetic clocks built from DNA methylation beadchip arrays, including the Illumina Infinium Human Methylation 450K and EPICv1 BeadChips, have been developed and are used to evaluate biological aging. However, there is still limited information on effectively utilizing the novel EPICv2 arrays and addressing biases in epigenetic clock calculations resulting from missing probes initially present on legacy arrays. We address how the absence of probes on the EPICv2 array, originally present on the 450K array, affects many available epigenetic clock estimates. Using data from the InCHIANTI study of aging, our findings show that while clocks like Hannum and GrimAgeV2 exhibit significant over- or underestimation due to missing probes, the correlations between estimates from all 450K probes (unreduced) and those from shared probes between 450K and EPICv2 (reduced) remain strong. In this cohort, we consider AgeAcceleration from traditional epigenetic clocks to be the most reliable approach. Their values do not significantly change upon probe reduction, with unreduced and reduced values being nearly identical and with stable mean differences across chronological age.
Comparing The Impact of Systemic Pituitary Adenylate-Cyclase-Activating Polypeptide (PACAP) and Calcitonin Gene-Related Peptide (CGRP) on Motion-Induced Nausea And Balance Behaviors In Mice
Authors: Rahman Shafaqat M. , Dweh Abigail , Luebke Anne E. 
Published: October 2024

AbstractPituitary adenylate-cyclase-activating polypeptide (PACAP), particularly its dominant isoform PACAP-38, is implicated in migraine and represents a promising therapeutic target. We investigated if intraperitoneally delivered (IP) PACAP-38 impacts motion-induced nausea, postural sway, and imbalance in C57BL/6J wildtype mice using the motion-induced thermoregulation, center of pressure (CoP), rotarod, and balance beam assays. We also assessed systemic Calcitonin Gene-Related Peptide’s (CGRP) effects on these behaviors in parallel. Our findings indicate that IP PACAP-38 significantly disrupts motion-induced thermoregulation in mice, with notable blunting of tail vasodilation responses in both sexes. Additionally, PACAP-38 administration increased postural sway in female mice only and caused balance beam imbalances. Contrary to IP CGRP, IP PACAP-38 did not affect rotarod performance when mice were trained on a dowel with 1.5 cm radius. Our findings provide preclinical evidence supporting a potential role of PACAP-38 in vestibular migraine pathophysiology. Future research will explore if PACAP antagonism can protect against PACAP-38s effects on nausea and balance behaviors, relevant to treatment of vestibular migraine (VM), especially for patients unresponsive to triptans or CGRP-targeting therapies.
Nitrogen ionome dynamics on leafy vegetables in tropical climate
Authors: Shenhar Itamar , Harikumar Aravind , Pebes Trujillo Miguel Raul , Zhao Zhitong , Lin Qin , Setyawati Magdiel Inggrid , Tan David , He Jie , Herrmann Ittai , Ng Kee Woei , Gavish Matan , Moshelion Menachem 
Published: October 2024

AbstractNitrogen is known to be a critical macro-nutrient influencing plant physiology, growth, and mineral composition. In tropical conditions, which are challenging for leafy vegetable farming, the nitrogen delivery effect is unclear. In this study, we aimed to investigate the effect of nitrogen application on key physiological traits and the mineral composition of the plants, the plant ionome. Experiments were conducted under tropical conditions greenhouse with varying levels of nitrogen supply to examine the effect on plant transpiration, yield, use efficiency of water and nitrogen, and nutrient uptake dynamics followed by cross-correlation analysis, trying to understand the physiological behavior-uptake dynamics relationships. The results demonstrated that transpiration, yield and WUE theoretic optimum curve, which peaking in nitrogen concentration of 120 mg/L for Chinese spinach and 200 mg/L for Chinese broccoli. Conversely, NUE reduce significantly with increasing nitrogen delivery which reflected on antagonistic increase of excess nitrogen.In terms of mineral composition, nitrogen application resulted in an increase I nitrogen content in the plant leaf tissue, while concentration of certain macronutrients and micronutrients were affected, including potassium, phosphorus, calcium, magnesium, iron, zinc, and molybdenum. Part of the minerals exhibited decreasing pattern due to potential competitive uptake mechanism, iron revealed increasing pattern that correlated with nitrogen delivery, and some minerals correlated with the measured physiological parameters. These results underscore the importance of optimizing nitrogen fertilization to balance plant growth, physiological processes, and plant nutrient homeostasis. The study offers valuable insights for sustainable nitrogen management in agricultural systems aimed at maximizing crop yield while maintaining nutritional quality.
Developmental changes in the control of primary motoneuron excitability by the M-current in larval zebrafish
Authors: Gaudreau Stephanie F. , Bui Tuan V. 
Published: October 2024

ABSTRACTSpinal circuits for locomotion undergo maturation during early development. How intrinsic properties of individual spinal neuron populations change throughout motor maturation is not fully understood. Here we identify for the first time the presence of the persistent outward potassium current known as the M-current in primary motoneurons of larval zebrafish. We show that the M-current controls excitability of primary motoneurons and its role in excitability control changes during development such that the magnitude of the M-current in primary motoneurons transiently increases at 3 days post-fertilization. These findings reveal a novel mechanism by which control over excitability of primary motoneurons in larval zebrafish is ensured, underscoring developmental changes in ion current contributions to intrinsic properties. Broadly, these data support the M-current as a conserved means to control motoneuron excitability across vertebrates.
A new synonym forViburnum erosum(Viburnaceae) in East China, based on morphological and molecular evidence
Authors: Zhao Liaocheng , Wang Yitian , Lyu Wenjun , Tang Zijian , Qiu Lihong , Tang Ming 
Published: October 2024

AbstractThe critical observations of living plants in the field, along with the examination of type specimens and protologues, led us to conclude that the key characteristics, including the length of the petiole, stipules and peduncles, as well as the shape of leaves ofViburnum fengyangshanense, all fall within the variation range ofV. erosum. Additionally, molecular analysis of nuclear ribosomal internal transcribed spacer (nrITS) and three plastid DNA markers (rbcL, matKandndhF) indicates thatV. fengyangshanenseandV. erosumare deeply nested within a clade. Therefore, based on morphological and molecular evidence, it is demonstrated thatV. fengyangshanenseshould be regarded as a new synonym ofV. erosum.
Male-specific sNPF peptidergic circuits control energy balance for mating duration through neuron-glia interactions
Authors: Zhang Xiaoli , Miao Hongyu , Kang Dayeon , Sun Dongyu , Kim Woo Jae 
Published: October 2024

ABSTRACTThis study reveals a sexually dimorphic sNPF-sNPF-R circuit in theDrosophilabrain that regulates energy balance behavior, specifically the shorter-mating-duration (SMD) response to sexual experience. sNPF is predominantly expressed in neurons, while sNPF-R is expressed in both neurons and glial cells, particularly astrocyte-like glia (ALG) in a subset of cells outside the mushroom body (MB) termed "Rishi cells" (RS cells). Sexual experience induces global alterations in calcium signaling and synaptic plasticity within this circuit, with sNPF-R expressing neurons and glia in RS cells playing a critical role in encoding sexual experience-related information into energy balance behavior related to mating duration. Neuronal glucose metabolism, specifically theTret1ltransporter, is essential for maintaining the high calcium levels and proper function of sNPF neurons near RS cells. This novel and intricate neuron-glia sNPF-sNPF-R network acts as critical circuits within theDrosophilabrain for processing interval timing behaviors, highlighting the dynamic interplay between metabolism, neural circuits, and behavior in regulating energy balance.
Quantification of Signal Amplification for Receptors: TheKd/EC50Ratio of Full Agonists as a Gain Parameter
Authors: Buchwald Peter 
Published: October 2024

AbstractConcentration-response relationships connecting the concentration of ligands to the responses they produce are central to pharmacology in general and form the core of quantitative pharmacology. While typically they can be well-described by hyperbolic functions (sigmoid on commonly used semi-log scales) and characterized by half-maximal concentrations values (EC50), their connection to receptor occupancy, characterized in a similar manner by the equilibrium dissociation constantKd, can be complex due to the intermixing of the effects from occupancy-induced activation with those from partial agonism, constitutive activity, and pathway-specific signal amplification. Here, it is proposed that, as long as both occupancy and response follow such typical concentration-dependencies, signal amplification can be quantified using the gain parametergK=κ=Kd/EC50measured for full agonists. This is similar to the gain parameter used in electronics (e.g.,gV=Vout/Vinfor voltage). On customarily used semi-log representations, loggKcorresponds to the horizontal shift between the response and occupancy curves, logKd-logEC50, the presence of which (i.e.,Kd>EC50) is generally considered as evidence for the existence of “receptor reserve” or “spare receptors”. The latter is a misnomer that should be avoided since even if there are excess receptors, there is no special pool of receptors “not required for ordinary use” assparewould imply. For partial agonists, theκ=Kd/EC50shift is smaller than for full agonists as not all occupied receptors are active. ThegKgain parameter (full agonistKd/EC50) correspond to theγgain parameter of the SABRE receptor model; for partial agonists (ε<1), SABRE predicts a corresponding shift ofκ=εγ-ε+1.
Wideband ratiometric measurement of tonic and phasic dopamine release in the striatum
Authors: Gottschalk Amy , Menees Haley , Bogner Celine , Zewde Semele , Jibin Joanna , Gamam Asma , Flink Dylan , Mosissa Meea , Bonneson Faith , Wehelie Hibo , Alonso-Caraballo Yanaira , Hamid Arif A. 
Published: October 2024

SummaryReward learning, cognition, and motivation are supported by changes in neurotransmitter levels across multiple timescales. Current measurement technologies for various neuromodulators (such as dopamine and serotonin) do not bridge timescales of fluctuations, limiting the ability to define the behavioral significance, regulation, and relationship between fast (phasic) and slow (tonic) dynamics. To help resolve longstanding debates about the behavioral significance of dopamine across timescales, we developed a novel quantification strategy, augmenting extensively used carbon-fiber Fast Scan Cyclic Voltammetry (FSCV). We iteratively engineered the FSCV scan sequence to rapidly modify electrode sensitivity within a sampling window and applied ratiometric analysis for wideband dopamine measurement. This allowed us to selectively eliminate artifacts unrelated to electrochemical detection (i.e., baseline drift), overcoming previous limitations that precluded wideband dopamine detection from milliseconds to hours. We extensively characterize this approachin vitro, validate performancein vivowith simultaneous microdialysis, and deploy this technique to measure wideband dopamine changes across striatal regions under pharmacological, optogenetic, and behavioral manipulations. We demonstrate that our approach can extend to additional analytes, including serotonin and pH, providing a robust platform to assess the contributions of multi-timescale neuromodulator fluctuations to cognition, learning, and motivation.
Pelvic Ring Fractures: Does Spinopelvic Alignment Affect Sacral and Lumbopelvic Fixation Stability?
Authors: Tripathi Sudharshan , Kumaran Yogesh , Soehnlen Sophia M. , Kelkar Amey , Seki Toshihiro , Sakai Takashi , Goel Vijay , Quatman Carmen E. , Nishida Norihiro 
Published: October 2024

AbstractBackground ContextPelvic ring is a complex boney and ligamentous complex and its structural integrity is paramount to be able to withstand the high forces. Pelvic ring fractures, which can result from low-energy impacts or high-velocity injuries and falls from great heights, can severely impair the structural integrity of the pelvic ring. These injuries can be fatal, especially in geriatric patients, with mortality rates ranging between 10% and 16%. Pelvic ring injuries require immediate stabilization, typically performed through internal fixation, to maintain circulatory balance and achieve anatomical restoration of the pelvis.MethodsA previously validated FE model of the spine, pelvis, and femur model was used to evaluate how different fixation techniques for pelvic ring injury affect spinopelvic biomechanics. The SS, PI, PT and LL of the normal, intact model was modified to generate two more SS models for this study. One modified model with an SS of 20º was considered the low SS model (SSD), while the second modified model with an SS of 32º was termed the high SS model (SSI). A unilateral pelvic ring fracture was simulated by resecting the left side of the sacrum and pelvis. The fractures were stabilized using two different posterior stabilization techniques 1) The fractures were stabilized using two different posterior stabilization techniques, and 2) L5-Ilium posterior screw fixation without cross connector (L5_PF_WO_CC).ResultsSSI demonstrated the higher range of motion, followed by Normal model and least by SSD model at right Sacroiliac Joint. No differences were seen between with and without cross connector model for range of motion at right SIJ. For L5-S1 motion models treated with cross connectors demonstrated the least motion compared to those treated without cross connectors for all configurations and loading conditions. For the sacrum fracture, models with high sacral slopes recorded the least horizontal displacement, followed by decreased sacral slope models for both configurations and loading conditions.
ProtNote: a multimodal method for protein-function annotation
Authors: Char Samir , Corley Nathaniel , Alamdari Sarah , Yang Kevin K. , Amini Ava P. 
Published: October 2024

AbstractUnderstanding the protein sequence-function relationship is essential for advancing protein biology and engineering. However, fewer than 1% of known protein sequences have human-verified functions. While deep learning methods have demonstrated promise for protein function prediction, current models are limited to predicting only those functions on which they were trained. Here, we introduce ProtNote, a multimodal deep learning model that leverages free-form text to enable both supervised and zero-shot protein function prediction. ProtNote not only maintains near state-of-the-art performance for annotations in its train set, but also generalizes to unseen and novel functions in zero-shot test settings. We envision that ProtNote will enhance protein function discovery by enabling scientists to use free text inputs, without restriction to predefined labels – a necessary capability for navigating the dynamic landscape of protein biology.