bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring You-gui Pill for the Treatment of Diabetic Erectile Dysfunction: Data Mining Analysis, Network Pharmacology and Experiments In Vitro
Authors: Chen Jiaqi , Gao Yanan , Zhang Yanqiu , Sun Yue , Jiang Yue , Yang Yong , Wang Mingxing 
Published: October 2024

Introduction:
The You-gui pill (YGP) is a classical compound used for treating antidiabetic
erectile dysfunction (DMED). However, the specific active ingredients responsible for
its effects on DMED and their mechanisms remain unclear.

Methods:
In this paper, we used data mining techniques to analyze high-frequency herbs and
herb combinations used in Chinese medicine for the treatment of DMED based on existing literature.
Using network pharmacology to study the active components and mechanism of action
of YGP against DMED, molecular docking was used to analyze the interactions of the active
components with major structural proteins, nonstructural proteins, and mutants. Also, the therapeutic
effect of YGP on hyperglycemic modelling and its underlying mechanisms were experimentally
validated in CCEC cells by analyzing the expression of its relevant target mRNAs.

Results:
Network pharmacological analysis identified the three core components of YGP as
quercetin, kaempferol, and β-sitosterol, and constructed a PPI network map of common targets
of YGP and DMED, which included HIF-1α, ALB, Bcl-2, INS, IL-1β, IL-6, TNF-α, CASP3,
and TP53. Combined with molecular docking results, these targets had a strong binding affinity
between them and the active ingredient compounds, with the highest affinity for HIF-1α and
TNF-α. During the in vitro cellular assay validation, the HIF-1α, ALB, Bcl-2, TNF-α, and
IL-6 mRNA in CCECs cells showed positive regulation after YGP intervention.

Conclusion:
The combination of "data mining - network pharmacology - molecular docking -
experimental validation" provides a powerful methodological basis for the study of the main active
components and mechanism of action of YGP against DMED, as well as the development
and application of the drug.

conclusion:
The combination of &quot;data mining - network pharmacology - molecular docking - experimental validation&quot; provides a powerful methodological basis for the study of the main active components and mechanism of action of YGP against DMED, as well as the development and application of the drug.
The Effect of Systemic Inflammatory Response on Mechanical Thrombectomy
is Partly Mediated by Pre-thrombectomy Cerebral Edema in Acute
Stroke Patients
Authors: Kan Yuan , Yang Lu , Ren Changhong , Li Chuanhui , Xu Jiali , Guo Wenting , Zhao Wenbo , Ji Xunming 
Published: October 2024

Objective:
To explore the effect of baseline Systemic Inflammatory Response reflected
by platelet-to-lymphocyte ratio (PLR) and pre-thrombectomy cerebral edema reflected by Net Water
Uptake (NWU) on futile recanalization in patients with Acute Ischemic Stroke (AIS) after successful
thrombectomy, and to investigate the potential mediating role of baseline cerebral edema.

Methods:
134 Patients with anterior circulation ischemic stroke receiving successful thrombectomy
were retrospectively studied. Their demographic and clinical characteristics were collected at
admission, and the NWU was quantitatively calculated based on baseline computed tomography
(CT). The predictive value of PLR for futile recanalization and the relationship between PLR,
NWU, and futile recanalization using mediation analysis were explored. Patients were followed
up for 90 days and were divided into a futile recanalization group and a favorable prognosis group
[90-day modified Rankin Scale score of 0–2].

Results:
High baseline PLR, NWU, no first-pass reperfusion, and large baseline ischemic core volume
were independent predictors of futile recanalization after successful thrombectomy in patients
with AIS. Mediation analysis results indicate that PLR may partially mediate the occurrence
of futile recanalization through NWU.

Conclusion:
Baseline PLR and NWU were independent predictors of futile recanalization, and
higher PLR and NWU values were associated with a higher likelihood of futile recanalization.
The findings suggest that early cerebral edema reflected by a high NWU value may be a mediator
of PLR-affecting prognosis.
Exploring the Antitumor Potential of New Indazole-indolizines Designed by Molecular Hybridization
Authors: Ciurla-Lucescu Liliana , Bîcu Elena , Belei Dalila , Ghinet Alina 
Published: October 2024

Background:
Cancer represents the major health problem faced by the population of the
world, remaining one of the main causes of death. Hence, the development of new targeted antitumor
drugs with high efficacy and lower toxicity is still needed.

Objective:
As a continuation of our work to discover new molecules with cytotoxic properties, two
heterocyclic scaffolds, namely indolizine, and indazole, were combined in the same molecule, aiming
to improve the bioactivity. This article focused on the synthesis, characterization, and biological
evaluation of a series of new indazole-indolizine hybrid compounds.

Methods:
The biological potential of the synthesized compound was investigated in vitro against the
human farnesyltransferase enzyme and NCI 60 tumor cell lines panel. While the farnesyltransferase
inhibitory activity was modest, a very good antiproliferative action was observed for compound 4a,
which, at a concentration of 10 μM, inhibited the growth of 20 types of cancer cells by more than
50% and showed cytotoxic action against the ovarian cancer cell line OVCAR-4.

Results:
A series of novel indazole-indolizine hybrids were synthesized via a [3+2] cycloaddition
reaction, fully characterized and biologically evaluated for antitumor potential.

Conclusion:
Compound 4a could be a promising starting point in the development of new antitumoral
agents. Further biological investigations will be performed to identify the biological target of
the compounds. Moreover, different synthetic strategies to introduce new substituents on the indolizine
core will be addressed.
Antenatal Care Services Uptake and Associated Factors in Somaliland: Further Analysis of the 2020 Somaliland Demographic Health Survey
Authors: Abdiwali Saad A. , Adesina Olubukola A. , Fekadu Gedefaw A. 
Published: October 2024

Purpose
Antenatal care (ANC) is vital to maintain the health of pregnant women and their unborn babies. It was linked to a positive pregnancy experience. However, ANC uptake remained low in Somaliland, and no study has been conducted to assess possible predictors. Hence, the current study aimed to determine the level of ANC utilization and to identify its associated factors in Somaliland.

Materials and Methods
Somaliland Demographic Health Survey (SLDHS) Data 2020 were used. A weighted sample of 3,192 women aged 15-49 years participated in the study. The survey used a two-stage cluster design for sample selection. Descriptive statistics in the form of frequency and percentage and multilevel multinomial logistic regression analysis were carried out to determine associated factors and statistical significance was declared by p< 0.05.

Results
The findings showed that 62.4% (95% CI: 60.7-64.1) of the women did not utilize ANC services, while 23.4% (95% CI: 21.9, 24.9) partially utilized and 14.2% (95% CI: 13.0, 15.4) adequately utilized ANC services. The odds of adequately utilizing ANC service decreased by a factor of 0.88 for women aged 35 and above compared to 15 - 24 year old women (AOR= 0.88, 95% CI: 0.61, 1.26). Women with higher educational levels had significantly higher odds (AOR=4.31, 95% CI: 1.54, 12.02) of adequately utilizing ANC compared to those with no formal education. The employment status, marital status, household wealth index, residence and region were also found to be associated with the utilization of Antenatal care.

Conclusion
The study showed low adequate utilization of ANC services in Somaliland. The variables, including age, education, wealth index, marital status, employment status, residence and region, were significantly associated with ANC utilization. Governmental and non-governmental organizations should enact a strategy with targeted intervention focusing on women from nomadic and rural communities, those from poor households, and low educational levels.
PNU-74654 Enhanced the Antiproliferative Activity of Gemcitabine by Targeting Wnt/β-Catenin Pathway in Pancreatic Cancer
Authors: Sharifi Sajjad , Mehrabadi Shima , Rahmani Farzad , Nosrati-Tirkani Abolfazl , Khorrami Shadi , Khazaei Majid , Hassanian Seyed Mahdi , Rajabian Majid , Boromand Nadia , Parizadeh Seyed Mohammad Reza , Ferns Gordon A. , Avan Amir 
Published: October 2024

Background:
The Wnt/beta-catenin pathway is one of the pathways that is deregulated
in pancreatic cancer and is reported to be associated with a poor prognosis. This indicates the
need for the identification of novel agents to improve the efficacy of current therapy or have an
improved efficacy. Therefore, in the present study, we explored the anticancer activity of PNU-
74654 alone or in combination with gemcitabine in 2 and 3-dimensional cell culture models of
pancreatic cancer.

Methods:
The MTT assay was carried out to determine the viability of PC cancerous cells
(PCCs), while the cytotoxicity of this agent was evaluated in a 3D cell culture model (spheroid).
The effects of PNU-74654 were investigated in established cell migration/invasion assays.

Results:
The expression of candidate genes affecting the cell cycle, migration, and Wnt/bcatenin
pathway was evaluated at mRNA and/or proteins by RT-PCR or Western blot. PNU-
74654 inhibited the cell growth at IC50 of 122 ± 0.4 umol/L and had a synergistic effect on the
antiproliferative properties of gemcitabine by modulating the Wnt pathway. The PNU-
74654/gemcitabine combination reduced the migratory and invasiveness of PC cells, compared
to control cells, through perturbation of E-cadherin.

Conclusion:
Our findings demonstrate the profound antitumor properties of PNU-74654 in in
vitro models of pancreatic cancer, supporting further in vivo studies to evaluate the therapeutic
impact of this novel therapy to target the Wnt pathway in the treatment of pancreatic cancer.
Antibiotic Prescribing Behavior of Physicians in Outpatient Departments in Hospitals in Northwest Ethiopia: Structural Equation Modeling Approach
Authors: Abejew Asrat Agalu , Wubetu Gizachew Yismaw , Fenta Teferi Gedif 
Published: October 2024

Background
Antibiotic resistance, fueled by irrational prescribing, is a global threat associated with health, social, and economic consequences. Understanding antibiotic prescribing behavior and associated factors is important to promote good prescribing practice.

Objective
This study aimed to determine the factors affecting antibiotic prescribing behaviors of physicians based on the theory of planned behavior in hospitals in northwest Ethiopia in 2022.

Methods
A cross-sectional study was conducted from September 2022 to October 2022. A total of 185 health professionals were included, and a self-administered questionnaire was used to collect data. A structural equation model based on the modified theory of planned behavior was used to determine factors affecting antibiotic prescribing behavior. The percentages of physicians’ estimated prescriptions for patients with upper respiratory tract infections (URTIs) and during weekly outpatient visits were used to predict antibiotic prescribing behavior and finally linked with behavioral constructs. A P value <.05 was considered significant.

Results
Physicians estimated that they prescribed antibiotics for 54.8% (9896/18,049) of weekly outpatient encounters, and 178 (96.2%) of the 185 physicians estimated they prescribed antibiotics for patients who presented with symptoms of a URTI. Physicians aged ≤30 years were less likely to prescribe antibiotics (48/100, 48%) for patients who presented with a URTI than physicians older than 30 years (51/100, 51%; P=.004), and general practitioners were less likely to prescribe antibiotics (47/100, 47%) for patients who presented with a URTI than residents (51/100, 51%; P=.03). Similarly, during outpatient visits, physicians ≤30 years old were less likely to prescribe antibiotics (54/100, 54%) than physicians older than 30 years (57/100, 57%; P<.001), male physicians were less likely to prescribe antibiotics (53/100, 53%) than female physicians (64/100, 64%; P=.03), and general practitioners were less likely to prescribe antibiotics (53/100, 53%) than residents (57/100, 57%; P=.02). Physicians with good knowledge were less affected by perceived social pressure (mean 4.4, SD 0.6) than those with poor knowledge (mean 4.0, SD 0.9; P<.001) and felt it was easy to make rational decisions (mean 4.1, SD 1.1) compared with those with poor knowledge (mean 3.8, SD 1; P<.001). However, intentions to reduce and prescribe antibiotics were not affected by attitudes, subjective norms, or perceived behavioral control, and perceived antibiotic prescribing behavior was not related to intentions to reduce or prescribe antibiotics.

Conclusions
Antibiotic prescribing behavior was not under the volitional control of physicians. This calls for a systematic approach to change antibiotic prescribing practices in hospital.
Efectos de la implementación de actividades prácticas sobre la motivación de logro en estudiantes universitarios
Authors: García-Garay Jonathan , Andrade-Mayorga Omar , Lavados-Romo Pamela 
Published: October 2024

Introducción: Motivación es aquello que impulsa a un sujeto hacia un objetivo que supone acciones, aceptando el esfuerzo requerido para conseguirlo. Puede ser extrínseca, asociada a factores externos como reconocimiento o castigo; e intrínseca, relacionada a aquello que surge del propio individuo como la autorrealización y desarrollo personal. La motivación de logro es una forma de motivación intrínseca e incentiva la búsqueda de soluciones a problemas sin necesidad de recompensas. Constituye un paso previo al aprendizaje, es su motor, por ello en educación es fundamental utilizar estrategias que aumenten la motivación de logro de los estudiantes.
Objetivo: Evaluar los efectos de la incorporación de actividades prácticas de laboratorio en la enseñanza de la fisiología humana sobre las atribuciones de motivación de logro en estudiantes de kinesiología.
Método: Se evaluaron las atribuciones de motivación de logro en estudiantes universitarios al final de dos asignaturas de fisiología humana equivalentes, en dos semestres consecutivos, mediante la Escala Atribucional de Motivación de Logro Modificada (EAML-M). La primera asignatura se basó en actividades tradicionales, mientras que en la segunda se implementaron, además, actividades prácticas de laboratorio de fisiología del ejercicio.
Resultados: La dimensión de la EAML-M con mayor puntaje al finalizar ambas asignaturas fue “interés/esfuerzo”. Comparando los resultados de ambas asignaturas, se encontraron diferencias significativas en dos dimensiones, específicamente aumentos en el puntaje “tarea/capacidad” (4.2 ± 0.56 vs. 4.6 ± 0.56; P = 0.002) y “examen” (5 [5-5] vs. 5 [4-6]; P = 0.011) en la asignatura que incorporó actividades prácticas.
Conclusiones: Estos resultados sugieren que la implementación de actividades prácticas de laboratorio de fisiología del ejercicio podría mejorar las atribuciones de motivación de logro en estudiantes universitarios, específicamente en las dimensiones “tarea/capacidad” y “examen”. Además, se encontró una posible influencia del género en la motivación de los estudiantes, pues al realizar análisis por sexo, solo se encontraron mejorías en las mujeres.
IER3IP1 Mutations Cause Neonatal Diabetes Due to Impaired Proinsulin Trafficking
Authors: Montaser Hossam , Leppänen Sonja , Vähäkangas Eliisa , Bäck Nils , Grace Alicia , Eurola Solja , Ibrahim Hazem , Lithovius Väinö , Stephens Samuel B. , Barsby Tom , Balboa Diego , Saarimäki-Vire Jonna , Otonkoski Timo 
Published: October 2024

Immediate early response 3 interacting-protein 1 (IER3IP1) is an endoplasmic reticulum (ER) resident protein, highly expressed in pancreatic cells and the developing brain cortex. Homozygous mutations in IER3IP1 have been found in individuals with microcephaly and neonatal diabetes, yet the underlying mechanism causing β-cell failure remains unclear. Here, we used differentiation of genome-edited stem cells into pancreatic islet cells to elucidate the molecular basis of IER3IP1 neonatal diabetes. Using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9), we generated two distinct IER3IP1 mutant human embryonic stem cell lines: a homozygous knock-in model of a patient mutation (IER3IP1V21G), and a knockout (KO) model (IER3IP1−/−). While these mutant stem cell lines differentiated normally into definitive endoderm and pancreatic progenitors, we observed that IER3IP1-KO stem cell-derived islets (SC-islets) presented a significant decrease in β-cell numbers and elevated ER stress. Retention using selective hooks assay revealed a threefold reduction in ER-to-Golgi trafficking of proinsulin in IER3IP1 mutant β-cells. Additionally, IER3IP1 mutant SC-islets implanted into immunocompromised mice displayed defective human insulin secretion, indicating the deleterious impact of IER3IP1 mutations on β-cell function. Our study provides valuable insights into the role of IER3IP1 in human β-cell biology and establishes a useful model to investigate ER-to-Golgi trafficking defects within β-cells.

Article Highlights
IER3IP1 mutations are linked to the development of microcephaly, epilepsy, and early-onset diabetes syndrome 1. However, the underlying molecular mechanisms of cell dysfunction are unknown. Using targeted genome-editing, we generated specific IER3IP1 mutations in human embryonic stem cell lines that were differentiated into pancreatic islet lineages. Loss of IER3IP1 resulted in a threefold reduction in endoplasmic reticulum-to-Golgi trafficking of proinsulin in stem cell-derived β-cells, leading to β-cell dysfunction both in vitro and in vivo. Loss of IER3IP1 also triggered increased markers of endoplasmic reticulum stress, indicating the pivotal role of the endoplasmic reticulum-to-Golgi trafficking pathway for β-cell homeostasis and function.
Drying Kinetics of Banana Chips: A Modeling Approach
Authors: Hermanuadi Didik , Iswahyono Iswahyono , Kurniawati Elly , Djamila Siti , Bahariawan Amal 
Published: October 2024

The primary goal of this research is to identify and evaluate the most suitable thin-layer drying model to effectively interpret the drying characteristics of banana chips and determine moisture diffusivity at different drying temperatures. The study utilized physiologically mature “kapok” bananas from the local market in Jember Regency. A flash dryer with a 4000-watt electric heating system was used, equipped with a blower for air circulation, an exhaust fan to expel water vapor. The bananas were processed into chips with a thickness of 1 mm. A total of 2000 g of banana chips were dried at constant temperature according to treatment conditions (air velocity 3.2 m/s, drying at temperatures of 60, 70, and 80°C). The study found that higher drying temperatures (80°C) achieved the highest initial drying rate (35.9% in 30 min) compared to 60°C (28.0%) and 70°C (22.0%). However, the drying rate gradually decreased at all temperatures. The drying kinetics of banana chips at 60, 70, and 80°C aligned well with the modified Midilli model. Effective moisture diffusivity values for banana chips at 60, 70, and 80°C were 4.947E-9 m²/s, 5.165(10–9) m²/s, and 5.756(10–9) m²/s, respectively, indicating that drying at 80°C was the most effective. The effective moisture diffusivity value showed a strong correlation with air velocity, drying temperature, material thickness, RH, and specific material attributes. Keywords: Banana, Diffusivity, Drying, Thin layer drying, Modified Midilli Model.
Design of Scheduled Fog Irrigation System with ESP 32 in Mustard Seedbed (Brassica juncea L.)
Authors: Sumarsono Joko , Widhiantari Ida Ayu , De Side Gagassage Nanaluih , Widhiantara I Ketut Manik 
Published: October 2024

Plants receive water from the fog irrigation system in the form of tiny, mist-like water particles. Currently, irrigation technology has advanced, nurseries can be automatically irrigated. In this research, the ESP32 microcontroller is used to create the electronic circuits for the automation of the fog irrigation control system in a mustard green nursery. This study's methodology combines direct field observation with an experimental or trial approach. Water flow (ml/minute), water use efficiency (%), plant height (cm), number of leaves, and scheduled fog irrigation system performance are among the measured parameters. In this investigation, four different treatments were used: manual irrigation using a watering can, two-time, three-time, and one-time irrigation. All is going according to plan with this mist watering system. With an average height of 4.58 cm and an average number of leaves of 3.24, the mist irrigation system had the highest water use efficiency of 77.35% during the test. It was also found that mist irrigation produced the highest amount of mustard leaves in the three-times-per-day treatment. Keywords: ESP32, Fog irrigation, Green mustard, Nurseries.
Performance Analysis of Disc Mill Type FFC-15 Grinder for Making Charcoal Husk Flour
Authors: Siskandar Ridwan , Kusumah Billi Rifa , Astuti Lusia Cipto 
Published: October 2024

This study is motivated by the great potential of rice husk as a waste material that has not yet been optimally utilized. The focus of this research is to evaluate the performance of the FFC-15 disc mill machine in producing flour from rice husk charcoal at three different moisture contents. The variations in the moisture content of rice husk charcoal studied were 10%, 15%, and 20%, with the goal of determining the optimal conditions in the production process of rice husk charcoal flour. The methodology used is experimental with repeated testing to produce data on machine capacity, electrical power consumption, flour yield, flour quality (moisture content, ash content, and mineral content), and the physical parameters of the charcoal. The results show that moisture content significantly affects the milling efficiency and quality of the flour produced, with optimal conditions achieved at a moisture content of 10%. Adjusting the moisture content before the milling process can enhance the operational efficiency of the machine and the quality of the final product. These findings are expected to provide benefits for follow-up by a group or industry that wants to optimize the utilization of rice husk charcoal. Keywords: Disc mill FFC-15, Flour quality, Husk charcoal, Milling efficiency, Moisture content.