bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Statistical, Photometric, and SED Analysis to Characterize the BSS Populations in Old Open Cluster: Berkeley 39
Authors: Sheikh A H , Medhi Biman J 
Published: October 2024

ABSTRACT
We present a statistical, photometric, and spectral energy distribution (SED) analysis to characterize the blue straggler stars (BSSs) populations in the Galactic old open cluster Berkeley 39. Berkeley 39 is a 6.16 Gyr old open cluster located at a distance of 3.99 Kpc.Gaia DR3 astrometry data have been used to estimate the membership probabilities using ensemble-based unsupervised machine learning techniques. We identified 21 BSS candidates on the colour–magnitude diagram, with 19 of them being detected in the Swift/UVOT UVW2 filter. We analysed the radial surface density profile and examined the cluster dynamical states and mass segregation effect. The SEDs of 19 BSSs are constructed using multiwavelength data covering UV to IR wavelengths. A single-component SED is fitted successfully for 14 BSS candidates. We discovered hot companions in five BSS candidates. These hot companions have temperatures of approximately 14 000 to 23 000 K, radii ranging from 0.04 to 0.13 R$_{\odot }$, and luminosities ranging from 0.16 to 2.91 L$_{\odot }$. Among these, three are most likely extremely low-mass white dwarfs (WDs) with masses around 0.17 to 0.18 M$_{\odot }$, and two are low-mass WDs with masses around 0.18 to 0.39 M$_{\odot }$. This confirms that they are post-mass transfer (Case A or Case B) systems. We also investigated the variable characteristics of BSSs by analysing their light curves using data from TESS. Our analysis confirms that two BSSs identified as eclipsing binaries in Gaia DR3 are indeed eclipsing binaries. Additionally, one of the two eclipsing binary BSSs shows evidence of having hot companions, as indicated by the multiwavelength SEDs.
Chromosomal integrons are genetically and functionally isolated units of genomes
Authors: Blanco Paula , Trigo da Roza Filipa , Toribio-Celestino Laura , García-Pastor Lucía , Caselli Niccolò , Morón Álvaro , Ojeda Francisco , Darracq Baptiste , Vergara Ester , Amaro Francisco , San Millán Álvaro , Skovgaard Ole , Mazel Didier , Loot Céline , Escudero José Antonio 
Published: October 2024

Abstract
Integrons are genetic elements that increase the evolvability of bacteria by capturing new genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs) can be massive and highly stabilized structures encoding hundreds of genes, whose function remains generally unknown. SCIs have co-evolved with the host for aeons and are highly intertwined with their physiology from a mechanistic point of view. But, paradoxically, other aspects, like their variable content and location within the genome, suggest a high genetic and functional independence. In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome of Vibrio cholerae N16961. We have relocated and deleted the SI using SeqDelTA, a novel method that allows to counteract the strong stabilization conferred by toxin–antitoxin systems within the array. We have characterized in depth the impact in V. cholerae’s physiology, measuring fitness, chromosome replication dynamics, persistence, transcriptomics, phenomics, natural competence, virulence and resistance against protist grazing. The deletion of the SI did not produce detectable effects in any condition, proving that—despite millions of years of co-evolution—SCIs are genetically and functionally isolated units of genomes.
Comparative Toxicogenomics Database’s 20th anniversary: update 2025
Authors: Davis Allan Peter , Wiegers Thomas C , Sciaky Daniela , Barkalow Fern , Strong Melissa , Wyatt Brent , Wiegers Jolene , McMorran Roy , Abrar Sakib , Mattingly Carolyn J 
Published: October 2024

Abstract
For 20 years, the Comparative Toxicogenomics Database (CTD; https://ctdbase.org) has provided high-quality, literature-based curated content describing how environmental chemicals affect human health. Today, CTD includes over 94 million toxicogenomic connections relating chemicals, genes/proteins, phenotypes, anatomical terms, diseases, comparative species, pathways and exposures. In this 20th year anniversary update, we reflect on CTD’s remarkable growth and provide an overview of the increased data content and new features, including enhancements to the curation workflow (e.g. new exposure curation tool and expanded use of natural language processing), added functionality (e.g. improvements to CTD Tetramers and Pathway View tools) and significant upgrades to software and infrastructure. Linking lab-based core curation with real-world human exposure curation via the use of controlled vocabularies facilitates analysis of content across the entire environmental health continuum, from molecular toxicological mechanisms to the population level, and vice versa. The ‘prototype database’ originally described in 2004 has evolved into a premier, sophisticated, highly cited and well-engineered knowledgebase and discoverybase that is utilized by scientists worldwide to design testable hypotheses about environmental health.
Disseminated histoplasmosis diagnosed on a blood smear in a Nigerian patient with non-Hodgkin’s lymphoma
Authors: Benjamin Ofonime O , Riman Omolabake E , Offiong Anthony , Egbara Walter O , Onukak Asukwo , Ogar Aje N , Ekeng Bassey E 
Published: October 2024

Abstract
Disseminated histoplasmosis is rarely reported in patients living with cancers in Nigeria. We report a 40-year-old woman who presented with left neck swelling and abdominal pain of two weeks duration. Clinical examination and radiological findings showed pallor, epigastric tenderness, generalized lymphadenopathy and hepatosplenomegaly. An initial diagnosis of sepsis and micronutrient deficiency was made following findings of macrocytosis, hypersegmented neutrophils and toxic granulations on blood smear. Intervention with antibiotics did not improve symptoms rather her clinical presentation worsened with the onset of fever, dizziness, easy fatiguability and generalized weakness. Histology of lymph node biopsy reported a diffuse large B-cell lymphoma. A repeat examination of the blood smear revealed budding yeast cells morphologically similar to Histoplasma capsulatum. This case emphasizes the need for a high index of suspicion of histoplasmosis in this at-risk population and the usefulness of a blood smear in diagnosing histoplasmosis.
Convergent Emergence of Glucomannan β-Galactosyltransferase Activity in Asterids and Rosids
Authors: Ishida Konan , Penner Matthew , Fukushima Kenji , Yoshimi Yoshihisa , Wilson Louis F L , Echevarría-Poza Alberto , Yu Li , Dupree Paul 
Published: October 2024

Abstract
β-Galactoglucomannan (β-GGM) is a primary cell wall polysaccharide in rosids and asterids. The β-GGM polymer has a backbone of repeating β-(1,4)-glucosyl and mannosyl residues, usually with mono-α-(1,6)-galactosyl substitution or β-(1,2)-galactosyl α-galactosyl disaccharide side chains on the mannosyl residues. Mannan β-galactosyltransferases (MBGTs) are therefore required for β-GGM synthesis. The single MBGT identified so far, AtMBGT1, lies in glycosyltransferase family 47A subclade VII and was identified in Arabidopsis. However, despite the presence of β-GGM, an orthologous gene is absent in tomato (Solanum lycopersicum), a model asterid. In this study, we screened candidate MBGT genes from the tomato genome, functionally tested the activities of encoded proteins and identified the tomato MBGT (SlMBGT1) in GT47A-III. Interestingly therefore, AtMBGT1 and SlMBGT1 are located in different GT47A subclades. Furthermore, phylogenetic and glucomannan structural analysis from different species raised the possibility that various asterids possess conserved MBGTs in an asterid-specific subclade of GT47A-III, indicating that MBGT activity has been acquired convergently among asterids and rosids. The present study highlights the promiscuous emergence of donor and acceptor preference in GT47A enzymes. The independent acquisition of the activity also suggests an adaptive advantage for eudicots to acquire β-GGM β-galactosylation and hence also suggests that the disaccharide side chains are important for β-GGM function.
Citrus yellow vein clearing virus infection triggers phloem remobilization of iron- and zinc-nicotianamine in citrus
Authors: Jiang Tianchi , Chen Jiuzhou , Xu Kuan , Sharon Bone , Li Luxi , Guo Jiansheng , Liu Lianghui , Ge Jun , Lin Haizhong , Tian Shengke , Lu Lingli 
Published: October 2024

Abstract
Citrus yellow vein clearing virus (CYVCV) is a worldwide and highly destructive disease of citrus, but the mechanisms involved in CYVCV-inhibited plant growth are not well understood. This study examined nutrient levels and their cellular distribution in different organs of healthy and CYVCV-affected citrus (Citrus reticulata ‘Kanpei’) plants. We found that CYVCV-infected plants exhibit characteristic symptoms, including a significant reduction in iron (Fe) and other elemental nutrients in the shoots. Our data suggest that CYVCV-induced chlorosis in citrus leaf veins is primarily due to iron deficiency, leading to reduced chlorophyll synthesis. Further analysis revealed a marked decrease in iron concentration within the pith and xylem of citrus petioles post-CYVCV infection, contrasting with increased Fe and zinc (Zn) concentrations in the phloem. Moreover, a substantial accumulation of starch granules was observed in the pith, xylem, and phloem vessels of infected plants, with vessel blockage due to starch accumulation reaching up to 81%, thus significantly obstructing Fe transport in the xylem. Additionally, our study detected an upregulation of genes associated with nicotinamide metabolism and Fe and Zn transport following CYVCV infection, leading to increased levels of nicotinamide metabolites. This suggests that CYVCV-infected citrus plants may induce nicotinamide synthesis in response to Fe deficiency stress, facilitating the transport of Fe and Zn in the phloem as nicotinamide-bound complexes. Overall, our findings provide insight into the mechanisms of long-distance Fe and Zn transport in citrus plants in response to CYVCV infection and highlight the role of nutritional management in mitigating the adverse effects of CYVCV, offering potential strategies for cultivating CYVCV-resistant citrus varieties.
Lower Extremity Osseointegration Postoperative Rehabilitation Protocols: A Scoping Review
Authors: Grunfeld Matan , Reif Taylor J , Rozbruch S Robert , Hoellwarth Jason S 
Published: October 2024

Abstract

Objective
Lower-extremity transcutaneous osseointegration is a rehabilitation alternative to socket-suspended prostheses. The rehabilitation process, philosophies, and routines remain under-described. This review, primarily, identifies commonalities and differences among protocols. Secondarily, strategies are proposed to streamline future research of post-osseointegration surgery rehabilitation.

Methods
Two differently-phrased queries of Google Scholar, Pubmed, Embase, and Web of Science were performed. First using either “osseointegration” or “osseointegrated” or “bone anchored prosthesis” AND [last name]. Second, replacing author name with “physical therapy” or “rehabilitation”. Six hundred eighty-eight articles were identified describing lower-extremity rehabilitation following osseointegration. Following software-based deduplication, manual abstract and full-text review, article reference evaluation, and use of Google Scholar’s “Cited by” feature, 35 studies were fully analyzed. First, a consolidated summary was made of protocols focusing on stages, timing, and other descriptions of postoperative rehabilitation. Subsequently, strengths and limitations of protocols were considered to propose potential strategies to investigate and optimize postoperative rehabilitation.

Results
All articles describe rehabilitation having this same order of goal progression: from surgery to gradual weight bearing and final goal of independent ambulation. The most impactful difference influencing the stated final goal of independent ambulation was whether one or two surgical stages were performed. No articles reported patient success rate achieving proposed goals and timing, or challenges during rehabilitation. Therefore, the first research suggestion is to investigate actual success rates achieving proposed goals and timing. Second, to further explore rehabilitation of performance deficits, beyond unaided ambulation. Finally, to incorporate technology such as mobility trackers to more objectively understand prosthesis use and mobility.

Conclusion
All lower-extremity osseointegration rehabilitation literature recommends identical goal progression order. No studies evaluate patient challenges or variation. Understanding and addressing such challenges may enhance postoperative rehabilitation.

Impact
This article consolidates published rehabilitation protocols post-osseointegration surgery. Specific analysis and experimentation of the protocols may enhance the uniformity and potential of patient rehabilitation.
Prospective associations between sleep, sensation-seeking, and mature screen usage in early adolescents: findings from the Adolescent Brain Cognitive Development study
Authors: Zhang Linhao , Oshri Assaf , Carvalho Cory , Uddin Lucina Q , Geier Charles , Nagata Jason M , Cummins Kevin , Hoffman Elizabeth A , Tomko Rachel L , Chaarani Bader , Squeglia Lindsay M , Wing David , Mason Michael J , Fuemmeler Bernard , Lisdahl Krista , Tapert Susan F , Baker Fiona C , Kiss Orsolya 
Published: October 2024

Abstract

Study Objectives
Early exposure to mature content is linked to high-risk behaviors. This study aims to prospectively investigate how sleep and sensation-seeking behaviors influence the consumption of mature video games and R-rated movies in early adolescents. A secondary analysis examines the bidirectional relationships between sleep patterns and mature screen usage.

Methods
Data were obtained from a subsample of 3687 early adolescents (49.2% female; mean age: 11.96 years) participating in the Adolescent Brain and Cognitive Development study. At year 2 follow-up, participants wore Fitbit wearables for up to 21 nights to assess objective sleep measures and completed a scale about sensation-seeking traits. At year 3 follow-up, they answered questions about mature screen usage.

Results
Of the sample, 41.8% of the sample reported playing mature-rated video games and 49% reported watching R-rated movies. Sensation-seeking traits were associated with R-rated movie watching 1 year later. Shorter sleep duration, later bedtime, more bedtime variability, and more social jetlag (discrepancy between the mid-sleep on weekdays and weekends) were associated with mature-rated video gaming and R-rated movie watching 1 year later. Sleep duration variability was associated with mature-rated video gaming. There was also an interaction effect: those with higher sensation-seeking scores and shorter sleep duration reported more frequent R-rated movie usage than those with longer sleep duration. Secondary analyses showed bidirectional associations between later bedtimes, more variability in bedtimes, and more social jetlag with mature screen usage.

Conclusions
Early adolescents with sensation-seeking traits and poorer sleep health were more likely to engage in mature screen usage.
Ephedrine attenuates LPS-induced M1 polarization of alveolar macrophages via the PKM2-mediated glycolysis
Authors: Xiang Yijin , Jiang Zaifeng , Yang Zhigang , Gong Shaomin , Niu Weiran 
Published: October 2024

Abstract

Background
Asthma is one of chronic inflammatory lung diseases in world. The important role of macrophage polarization and glycolysis in lung inflammation has attracted considerable attention. Ephedrine (EP) is a compound isolated from Ephedra and plays a regulatory role in inflammatory response, but its role in asthma and mechanism involved are not clear. Therefore, the purpose of this study was to investigate the molecular mechanism and effect of EP on lipopolysaccharide (LPS)-induced alveolar macrophage polarization and glycolysis.

Methods
We investigated the expression of Tnf-a, Nos2, Il10, and Arg1 using RT-PCR, as well as PKM2 and LDHA protein expression with Western blot. A CCK-8 assay was performed to determine the viability of the cells. The extracellular acidification rate (ECAR), ATP and lactate level were detected using commercial kits.

Results
The results revealed that EP alleviated LPS-induced NR8383 cell glycolysis and M1 polarization. Further studies found that EP enhanced the effect of 2-DG on NR8383 cell glycolysis and M1 polarization. More importantly, PKM2 inhibitor alleviated LPS-induced NR8383 cell glycolysis and M1 polarization. In addition, EP alleviated LPS-induced NR8383 cell glycolysis and M1 polarization by targeting PKM2.

Conclusion
It is suggested that EP alleviates LPS-induced glycolysis and M1 polarization in NR8383 cells by regulating PKM2, thereby alleviating lung injury, suggesting the involvment of alveolar macrophage polarization and glycolysis in the role of EP in asthma.
Lagged effects of climate factors on bacillary dysentery in western China
Authors: Li Rui , Liu Dongpeng , Wang Tingrong , Li Donghua , Shi Tianshan , Zhao Xin , Zheng Hongmiao , Ren Xiaowei 
Published: October 2024

Abstract

Background
Evidence has shown that the incidence of bacillary dysentery (BD) is associated with climatic factors. However, the lagged effects of climatic factors on BD are still unclear, especially lacking research evidence from arid and semi-arid regions. Therefore, this study aims to add new insights into this research field.

Methods
Spatial autocorrelation, time series analysis and spatiotemporal scans were used to perform descriptive analyses of BD cases from 2009 to 2019. On the basis of monthly data from 2015 to 2019, multivariable distributed lag non-linear models were used to investigate the lagged effects of climatic factors on BD.

Results
The hot spots for BD incidence are gradually decreasing and becoming increasingly concentrated in the southern part of Gansu Province. The maximum cumulative relative risks for monthly average temperature, sunshine duration, average relative humidity and precipitation were 3.21, 1.64, 1.55 and 1.41, respectively. The lagged effects peaked either in the current month or with a 1-month lag, and the shape of the exposure–response curve changed with the increase in maximum lag time. After stratification by per capita gross domestic product, there were differences in the effects.

Conclusions
Climatic factors can influence the incidence of BD, with effects varying across different lag times. It is imperative to vigilantly track the disparities in the incidence of BD attributable to economic factors.
More Than Body Parts: A New Ethos of Anatomy Education
Authors: Hildebrandt Sabine , Cornwall Jon , Champney Thomas H. 
Published: October 2024

Abstract
A new ethos of anatomy education goes beyond the learning of body parts in the traditional curriculum. In the traditional curriculum, the focus of only providing information on the structure of the human body left certain learning opportunities overlooked, marginalized, or dismissed as irrelevant; thus, opportunities to foster and shape professional attributes in health care learners were lost. Furthermore, changes in curricula structures and reductions in anatomy teaching hours have necessitated a transformation in how anatomy education is perceived and delivered. This article presents an examination of a contemporary ethos of anatomy education that draws together concepts from teaching theory, educational trends, and emerging research to maximize the anatomy learning experience with body donors. Social constructivism, the spiral curriculum, and the hidden curriculum can be leveraged to facilitate a unique educational experience. Furthermore, contemporary perspectives on the ethics and history of anatomy that support contextual learning indicate how these subjects are well suited to authenticate and consolidate learning, while supporting the relational and humanistic delivery of health care education. When anatomy education is conceptualized as a first clinical experience in a health care curriculum, it provides the opportunity to develop and enhance professional identity formation at a foundational stage. By embracing and incorporating a wider range of considerations into educational practice, a new ethos for anatomy education fosters history-informed professional identity formation that offers transformative learning opportunities for an ethical practice in the art of healing.
Association Between the Neutrophil-to-Lymphocyte Ratio and in-Stent Neoatherosclerosis and Plaque Vulnerability: An Optical Coherence Tomography Study
Authors: Gu Ning , Chen Panke , Wang Xi , Shen Changyin , Deng Yi , Chen Jianling , Ma Yi , Ma Shuai , Hu Xingwei , Zhao Ranzun , Shi Bei 
Published: October 2024

Abstract:
The aim of this study was to explore the relationship between in-stent neoatherosclerosis (ISNA) and the neutrophil-to-lymphocyte ratio (NLR) in patients with in-stent restenosis (ISR) following drug-eluting stent (DES) implantation. We divided 216 patients into 3 groups based on the NLR tertile. We performed a comparative analysis of baseline, angiographic, and features of optical coherence tomography (OCT) between the NLR groups and performed univariate and multivariate logistic regression analyses to assess the association of the NLR with ISNA and in-stent thin-cap fibroatheroma (TCFA). Patients in the third tertile NLR group had a higher incidence of ISNA and in-stent TCFA compared with those in the first tertile. Multivariate logistic regression analysis showed that the hazard ratios and 95% confidence intervals for ISNA and TCFA were 2.673 (1.257–5.684; P = 0.038) and 4.272 (1.740–10.488; P = 0.004), respectively, for patients in the highest tertile compared with those in the lowest tertile. Our study showed that an increased NLR was associated with ISNA and in-stent plaque fragility in patients with ISR following DES implantation.
Salvage Operation of Head and Neck Reconstruction Using a Pedicled Latissimus Dorsi Myocutaneous Flap with Distally Positioned Skin Paddle
Authors: Kojima Tsubasa , Motomura Hisashi , Nochi Deguchi Ayaka , Maeda Shusaku , Kang Songsu 
Published: October 2024

Background:
There are many situations in head and neck reconstruction where a pedicled flap must be chosen over free flaps. In such situations, one of our options is a pedicled latissimus dorsi (LD) myocutaneous flap with distally positioned skin paddle with a more distal skin flap design.

Methods:
We performed 22 reconstruction cases using a distally positioned pedicled LD myocutaneous flap. When designing the skin paddle more distally than usual, it is important to include the lateral cutaneous branch of the 10th posterior intercostal artery. By splitting the skin paddle sections, the LD flap is flexible for use in complex head and neck defects. It can also be used for mandibular reconstruction by harvesting the flap with costal bone.

Results:
In all cases, the skin flaps survived, with a salvage rate of 100%.

Conclusions:
Because elevation of this flap is relatively easy, safe, and quick, this flap selection is considered practical in salvage cases after flap necrosis, and in cases after multiple procedures.