bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth patterns of bacterial cultures depending on the nitrogen availability
Authors: Kan G.V. ,   Limnological Institute Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia, Suslova M.Yu. ,   Limnological Institute Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia, Tikhonova I.V. ,   Limnological Institute Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia, Lipko I.A. ,   Limnological Institute Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia, Belykh O.I. ,   Limnological Institute Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia
Published: September 2024

Morphological and ultrastructural growth patterns of bacterial cultures in response to the nitrogen availability were demonstrated using cultivation and scanning electron microscopy (SEM). It was found that strains of Pseudomonas sp. 1CB and Streptomyces sp. 21A are able to grow only in the medium with available nitrogen. Rhizobium sp. 2A formed biofilm on glass in medium with nitrate and in medium without available nitrogen. In a non-nitrogenous medium, an increase in the diameter of bacterial cells was observed due to the intensive secretion of polysaccharide mucus, which can be considered a mechanism of protection of the nitrogenase complex from the damaging effect of oxygen.
Rancang Bangun Monitoring Kualitas Air Budidaya Ikan Air Tawar Berbasis Mikrokontroler
Authors: Sujono Sujono , Wahyunugroho Syarif Nurullah 
Published: September 2024

Budidaya ikan air tawar merupakan sektor penting dalam industri perikanan dan akuakultur di Indonesia. Untuk menghasilkan kualitas ikan yang baik, maka diperlukannya monitoring kualitas air pada kolam, seperti memperhatikan pH, TDS, dan suhu. Kualitas air yang baik bagi ikan air tawar memiliki nilai pH 6,5-8, nilai TDS dibawah 1000ppm, dan nilai suhu sekitar 28-32οC. Di era teknologi yang terus berkembang, Pada tugas akhir ini bertujuan membuat sistem monitoring kualitas air kolam berbasis mikrokontroler dengan menggunakan sensor pH-4502c, sensor TDS, dan sensor suhu(ds18b20), mikrokontroler yang digunakan pada sistem ini adalah ESP32, sistem monitoring tersebut dapat memonitoring secara real-time dari jarak dekat maupun jarak jauh. Dalam perancangan sistem tersebut menggunakan metode penelitian Prototyping yaitu pengumpulan Kebutuhan sistem monitoring, proses desain, membangun prototipe dan melakukan ujicoba prototipe. Hasil dari percobaan sistem monitoring mendapatkan rata-rata presentase error yaitu sensor pH memiliki rata-rata error 3,17%, sensor TDS memiliki rata-rata error 2,67% dan sensor suhu memilliki rata-rata error 1,35%. Dari hasil presentase error pada sensor menunjukkan sistem monitoring sudah berjalan dengan baik dan dapat digunakan untuk memonitoring kualitas air pada budidaya ikan air tawar.
Rancang Bangun E-Book Budidaya Bawang Merah
Authors: Meiliawati Wanda , Nasirudin Mohamad , Sifaunajah Agus , Yuliana Anggi Indah 
Published: September 2024

Dalam rangka meningkatkan pengetahuan masyarakat terhadap bawang merah di wilayah Kabupaten Jombang, maka dibutuhkan inovasi-inovasi yang dapat memberikan dampak positif terhadap kemajuan bidang pertanian yang ada. Salah satu aspek yang dapat mendukung kemajuan adalah dengan adanya sistem informasi E-Book Komoditas Bawang Merah. Sistem E-Book Komoditas Bawang Merah adalah aplikasi berbasis web yang ditujukan kepada masyarakat terkait pengetahuan dan pemahaman tentang komoditas bawang merah di wilayah Kabupaten Jombang. Dari sistem tersebut, akan memberikan informasi yang terstruktur dan terorganisir tentang budidaya bawang merah dengan memanfaatkan pendekatan sistem informasi. Hal ini akan membantu petani atau pembudidaya dalam memahami proses budidaya secara menyeluruh dan langkah-langkahnya yang perlu diambil. Selain itu, sistem ini juga memberikan informasi terkait kondisi tanaman, umur tanaman dan petunjuk dalam penentuan tanaman agar sesuai dengan lahan yang ada. Untuk mewujudkan hal tersebut, penulis merancang dan membangun sistem informasi E-Book Komoditas Bawang Merah . Penelitian ini menggunakan metode R&D (Research and Development). Hasil pengujian menggunakan Black Box menunjukkan bahwa fungsionalitas dari sistem informasi ini telah berjalan dengan baik. Hasil akhir yang didapatkan dari penelitian ini adalah sistem informasi E-Book komoditas bawang merah memberikan wawasan bagi masyarakat untuk meningkatkan hasil pertanian bawang merah.
Analisis Potensi Pendapatan dan Kemandirian Daerah di Kawasan Arek Jawa Timur
Authors: Abdillah Khubbi 
Published: September 2024

The study aims to determine the magnitude of the potential for regional income and independence arek region in East Java Province in 2017 – 2021, by calculating the Location Quotient analysis, the degree of fiscal decentralization, and the regional fiscal capacity index. The results of the LQ calculation show that Batu City has the highest LQ in other service sectors. Analysis of the highest degree of fiscal decentralization and regional fiscal capacity index is owned by Surabaya City. The research results recommend that local governments need to optimize their economic potential as a source of tax revenue, increase local own-source revenue (PAD) so that they’re not dependent on transfers from central government, and increase regional capabilities by expanding a new tax base to finance government spending.
تحليل المحتوى اللغوي من كتاب التعليم “Bahasa Arab”للفصل السادس الابتدائي تأليف حلمي قاسم مباح ومحمد رضا مسلم على نظرية رشدي أحمد طعيمة
Authors: Muhammad Ghulam Alim , Abdul Basid  , Musta'in  
Published: September 2024

يهدف هذا البحث إلى معرفة مناسبة المحتوى اللغوية لكتاب التعليم “Bahasa Arab” للفصل السادس الابتدائي من تأليف حلمي قاسم مباح ومحمد رضا مسلم على نظرية رشدي أحمد طعيمة.هذا البحث بحث تحليلي ويحلل المحتوى الكتاب من حيث اللغة بمدخل الكمي. وجمعت البيانات بطريقة ملاحظة الكتاب والوثائق. نتيجة هذا البحث أن كتاب التعليم “Bahasa Arab” للفصل السادس الابتدائي تأليف حلمي قلسم مباح ومحمد رضا مسلم من حيث المحتوى اللغوي مناسب مع نظرية رشدي أحمد طعيمة في إعداد الكتاب بأسس إعداد الكتاب بالنسبة المئوية 76.25% بتقدير مناسب.
Conception vessel acupuncture research regularity in the treatment of diminished ovarian reserve: a multi-center, large-sample prospective cohort study protocol
Authors: Zhang Xiaoyu , Sun Hao , Zhang Na , Mou Zijun , Xia Qingchang , Du Dongqing , Ma Yuxia 
Published: September 2024

BackgroundDiminished ovarian reserve (DOR) refers to a decrease in the number or quality of oocytes in the ovarian cortex, which is a degenerative disease of the reproductive system, and can further develop into premature ovarian failure. There are few studies on acupuncture and moxibustion for DOR, which are still in the exploratory stage.Methods/designThis study was a real-world case registry study. According to whether the subjects received conception vessel acupuncture or not, they were divided into the basic treatment combined with conception vessel acupuncture group and the basic treatment group. A total of 1221 patients with DOR were enrolled and treated for 12 weeks. The percentage of patients with ≥30% improvement in anti-Müllerian hormone (AMH) was evaluated at the end of week 12. Secondary outcomes included Antral follicle count (AFC), modified Kupperman scale, basal FSH level, LH level, FSH/LH ratio, positive pregnancy, clinical pregnancy, early spontaneous abortion, ongoing pregnancy, and ectopic pregnancy.DiscussionThis study provides clinical evidence and theoretical support for the treatment of DOR with conception vessel acupuncture and moxibustion, so as to guide and improve the efficacy of acupuncture and moxibustion. Trial registrationAcupuncture-Moxibustion Clinical Trial Registry ChiCTR2400080471. Registered on 30 January 2024.
Increasing the Methane Potential of Oat Husks Using a Novel Extrusion Pre-Treatment Technology Prior to Anaerobic Digestion
Authors: Kirby Marie E. , Mirza Muhammad W. , Hoskyns-Abrahall Harry , Fenwick James , Theodorou Michael K. 
Published: September 2024

Oat husks are produced during the milling process of oats. Oat husks are a lignocellulosic material that have the potential for valorization thereby improving the circular economy of agricultural by-products. However, due to the high lignocellulosic content, there are limited valorization pathways for oat husks. To improve the anaerobic digestibility of oat husks, pre-treatment was investigated as a method to aid valorization. A novel extrusion process was used in an attempt to fragment the lignocellulosic structure of oat husks prior to anaerobic digestion. The extrusion pre-treatment was investigated to determine the effect it may have on altering the methane yield and digestibility of oat husks. Biochemical methane potential assays were undertaken using oat husks with no pre-treatment and extruded oat husks. These assays demonstrated that extruded oat husks produced a significantly higher methane yield of 264 ml/gVS fed, which was 27% greater than the methane yield produced from the untreated oat husks. Similarly, the total solids degradation was also significantly higher for extruded oat husks treatment compared to the untreated oat husks. Overall, the extrusion process demonstrated an increased methane yield for oat husks compared to previously published data. The biomethane potential tests suggest that extruded oat husks would be a feedstock suitable for anaerobic digestion.
Evidence of survival bias in the association between APOE-Є4 and age at ischemic stroke onset
Authors: von Berg Joanna , McArdle Patrick F. , Häppölä Paavo , Haessler Jeffrey , Kooperberg Charles , Lemmens Robin , Pezzini Alessandro , Thijs Vincent , Pulit Sara L. , Kittner Steven J. , Mitchell Braxton D. , de Ridder Jeroen , van der Laan Sander W. 
Published: September 2024

IntroductionLarge genome-wide association studies (GWASs) using case–control study designs have now identified tens of loci associated with ischemic stroke (IS). As a complement to these studies, we performed GWAS in a case-only design to identify loci influencing the age at onset (AAO) of ischemic stroke.MethodsAnalyses were conducted in a discovery cohort of 10,857 ischemic stroke cases using a linear regression framework. We meta-analyzed all SNPs with p-value &lt;1 x 10−5 in a sexcombined or sex-stratified analysis using summary data from two additional replication cohorts.ResultsIn the women-only meta-analysis, we detected significant evidence for the association of AAO with rs429358, an exonic variant in apolipoprotein E (APOE) that encodes for the APOE-Є4 allele. Each copy of the rs429358:T&gt;C allele was associated with a 1.29-year earlier stroke AAO (meta p-value = 2.48 x 10−11). This APOE variant has previously been associated with increased mortality and ischemic stroke AAO. We hypothesized that the association with AAO may reflect a survival bias attributable to an age-related decrease in mortality among APOE-Є4 carriers and have no association to stroke AAO per se. A simulation study showed that a variant associated with overall mortality might indeed be detected with an AAO analysis. A variant with a 2-fold increase in mortality risk would lead to an observed effect of AAO that is comparable to what we found.DiscussionIn conclusion, we detected a robust association of the APOE locus with stroke AAO and provided simulations to suggest that this association may be unrelated to ischemic stroke per se but related to a general survival bias.
The prognostic utility of 18F-FDG PET parameters in lymphoma patients under CAR-T-cell therapy: a systematic review and meta-analysis
Authors: Al-Ibraheem Akram , Abdlkadir Ahmed Saad , Al-Adhami Dhuha Ali , Sathekge Mike , Bom Henry Hee-Seung , Ma’koseh Mohammad , Mansour Asem , Abdel-Razeq Hikmat , Al-Rabi Kamal , Estrada-Lobato Enrique , Al-Hussaini Maysaa , Matalka Ismail , Abdel Rahman Zaid , Fanti Stephano 
Published: September 2024

BackgroundChimeric antigen receptor (CAR) T-cell therapy has attracted considerable attention since its recent endorsement by the Food and Drug Administration, as it has emerged as a promising immunotherapeutic modality within the landscape of oncology. This study explores the prognostic utility of [18F]Fluorodeoxyglucose positron emission tomography ([18F]FDG PET) in lymphoma patients undergoing CAR T-cell therapy. Through meta-analysis, pooled hazard ratio (HR) values were calculated for specific PET metrics in this context.MethodsPubMed, Scopus, and Ovid databases were explored to search for relevant topics. Dataset retrieval from inception until March 12, 2024, was carried out. The primary endpoints were impact of specific PET metrics on overall survival (OS) and progression-free survival (PFS) before and after treatment. Data from the studies were extracted for a meta-analysis using Stata 17.0.ResultsOut of 27 studies identified for systematic review, 15 met the criteria for meta-analysis. Baseline OS analysis showed that total metabolic tumor volume (TMTV) had the highest HR of 2.66 (95% CI: 1.52-4.66), followed by Total-body total lesion glycolysis (TTLG) at 2.45 (95% CI: 0.98-6.08), and maximum standardized uptake values (SUVmax) at 1.30 (95% CI: 0.77-2.19). TMTV and TTLG were statistically significant (p &lt; 0.0001), whereas SUVmax was not (p = 0.33). For PFS, TMTV again showed the highest HR at 2.65 (95% CI: 1.63-4.30), with TTLG at 2.35 (95% CI: 1.40-3.93), and SUVmax at 1.48 (95% CI: 1.08-2.04), all statistically significant (p ≤ 0.01). The ΔSUVmax was a significant predictor for PFS with an HR of 2.05 (95% CI: 1.13-3.69, p = 0.015).Conclusion[18F]FDG PET parameters are valuable prognostic tools for predicting outcome of lymphoma patients undergoing CAR T-cell therapy.
Prognostic cellular senescence-related lncRNAs patterns to predict clinical outcome and immune response in colon cancer
Authors: Cao Lichao , Chen Fang , Xu Long , Zeng Jian , Wang Yun , Zhang Shenrui , Ba Ying , Zhang Hezi 
Published: September 2024

BackgroundCellular senescence (CS) is believed to be a major factor in the evolution of cancer. However, CS-related lncRNAs (CSRLs) involved in colon cancer regulation are not fully understood. Our goal was to create a novel CSRLs prognostic model for predicting prognosis and immunotherapy and exploring its potential molecular function in colon cancer.MethodsThe mRNA sequencing data and relevant clinical information of GDC TCGA Colon Cancer (TCGA-COAD) were obtained from UCSC Xena platform, and CS-associated genes was acquired from the CellAge website. Pearson correlation analysis was used to identify CSRLs. Then we used Kaplan–Meier survival curve analysis and univariate Cox analysis to acquire prognostic CSRL. Next, we created a CSRLs prognostic model using LASSO and multivariate Cox analysis, and evaluated its prognostic power by Kaplan–Meier and ROC curve analysis. Besides, we explored the difference in tumor microenvironment, somatic mutation, immunotherapy, and drug sensitivity between high-risk and low-risk groups. Finally, we verified the functions of MYOSLID in cell experiments.ResultsThree CSRLs (AC025165.1, LINC02257 and MYOSLID) were identified as prognostic CSRLs. The prognostic model exhibited a powerful predictive ability for overall survival and clinicopathological features in colon cancer. Moreover, there was a significant difference in the proportion of immune cells and the expression of immunosuppressive point biomarkers between the different groups. The high-risk group benefited from the chemotherapy drugs, such as Teniposide and Mitoxantrone. Finally, cell proliferation and CS were suppressed after MYOSLID knockdown.ConclusionCSRLs are promising biomarkers to forecast survival and therapeutic responses in colon cancer patients. Furthermore, MYOSLID, one of 3-CSRLs in the prognostic model, could dramatically regulate the proliferation and CS of colon cancer.
Causal relationship between modifiable risk factors and knee osteoarthritis: a Mendelian randomization study
Authors: Diao Zhihao , Guo Danyang , Zhang Jingzhi , Zhang Ruiyu , Li Chunjing , Chen Hao , Ma Yuxia 
Published: September 2024

BackgroundWhile several risk factors for knee osteoarthritis (KOA) have been recognized, the pathogenesis of KOA and the causal relationship between modifiable risk factors and KOA in genetic epidemiology remain unclear. This study aimed to determine the causal relationship between KOA and its risk factors.MethodsData were obtained from published Genome-Wide Association study (GWAS) databases. A two-sample Mendelian randomization (MR) analysis was performed with genetic variants associated with risk factors as instrumental variables and KOA as outcome. First, inverse variance weighting was used as the main MR analysis method, and then a series of sensitivity analyses were conducted to comprehensively evaluate the causal relationship between them.ResultsUnivariate forward MR analysis revealed that genetically predicted hypothyroidism, hyperthyroidism/thyrotoxicosis, educational level, income level, metabolic syndrome (MS), essential hypertension, height, hot drink temperature, diet (abstaining from sugar-sweetened or wheat products), and psychological and psychiatric disorders (stress, depression, and anxiety) were causally associated with KOA. Reverse MR exhibits a causal association between KOA and educational attainment. Multivariate MR analysis adjusted for the inclusion of potential mediators, such as body mass index (BMI), smoking, alcohol consumption, and sex, exhibited some variation in causal effects. However, hyperthyroidism/thyrotoxicosis had a significant causal effect on KOA, and there was good evidence that height, hypothyroidism, educational level, psychological and psychiatric disorders (stress, depression, and anxiety), and abstaining from wheat products had an independent causal relationship. The mediating effect of BMI as a mediator was also identified.ConclusionThis study used MR to validate the causal relationship between KOA and its risk factors, providing new insights for preventing and treating KOA in clinical practice and for developing public health policies.
Antimicrobial and antibiofilm effects of cyclic dipeptide-rich fraction from Lactobacillus plantarum loaded on graphene oxide nanosheets
Authors: Dezaki Farid Shirmardi , Narimani Tahmineh , Ghanadian Mustafa , Bidram Elham , Poursina Farkhondeh 
Published: September 2024

IntroductionOne effective method to combat bacterial infections is by using bacteria itself as a weapon. Lactobacillus is a type of fermenting bacterium that has probiotic properties and has demonstrated antimicrobial benefits against other bacteria. Cyclodipeptides (CDPs), present in the supernatant of Lactobacillus, possess several antimicrobial properties.MethodsIn this study, the CDP fraction was isolated from the supernatant of Lactobacillus plantarum (L. plantarum). This fraction was then loaded onto graphene oxide nanosheets (GO NSs). The study assessed the substance’s ability to inhibit bacterial growth by using the minimum inhibitory concentration (MIC) method on A. baumannii and S. aureus strains that were obtained from clinical samples. To determine the substance’s impact on biofilm formation, the microtiter plate method was used. Moreover, the checkerboard technique was employed to explore the potential synergistic effects of these two substances.Results and discussionAccording to the study, the minimum inhibitory concentration (MIC) of the desired compound was found to be 1.25 mg/mL against S. aureus and 2.5 mg/mL against A. baumannii. Furthermore, at a concentration of 10 mg/mL, the compound prevented 81.6% (p &lt; 0.01) of biofilm production in A. baumannii, while at a concentration of 1.25 mg/mL, it prevented 47.5% (p &lt; 0.05) of biofilm production in S. aureus. The study also explored the synergistic properties of two compounds using the checkerboard method.ConclusionIn general, we found that GO NSs possess antimicrobial properties and enhance cyclodipeptides’ activity against S. aureus and A. baumannii.
Insights into the airborne microorganisms in a Sichuan south-road dark tea pile fermentation plant during production
Authors: Liu Miaoyi , Li Xian , Li Yimiao , Zou Yao 
Published: September 2024

IntroductionSichuan south-road dark tea (SSDT) is generally produced through a series of processes, including fixing, rolling, pile fermentation, and drying, with microbial action during pile fermentation playing a crucial role in determining tea quality. The air within the SSDT pile fermentation plant (SSDTPP) is considered an important source of these microbes, but research in this area has been limited.MethodsIn this study, air samples from SSDTPP were collected on the 1st (SSDT1), 12th (SSDT2), and 24th (SSDT3) days of pile fermentation and comprehensively analyzed by high-throughput sequencing.Results and discussionThe results revealed the presence of 2 and 24 phyla, 9 and 49 classes, 18 and 88 orders, 28 and 153 families, 38 and 253 genera, and 47 and 90 species of fungi and bacteria, respectively, across all samples. SSDT1 and SSDT2 individually had the highest fungal and bacterial diversity, while Aspergillus was the dominant genus throughout the pile fermentation with an abundance of 34.6%, 91.17%, and 67.86% in SSDT1, SSDT2, and SSDT3, respectively. Microbial populations in SSDT1 were predominantly involved in xenobiotic biodegradation and metabolism, amino acid metabolism, the biosynthesis of other secondary metabolites, etc. However, SSDT2 exhibited a higher prevalence of human disease-related functions. SSDT3 primarily focused on the metabolism of other amino acids and carbohydrate metabolism. Additionally, 104 genera and 22 species coexisted in both SSDTPP air and piled SSDT, suggesting that frequent microbial exchange may occur between them. These findings pave the way for microbial traceability during SSDT production and provide a foundation for further functional microbial research.