bluuebunny/crossref_metadata_2025_split huggingface dataset:
Halotherapy as asthma treatment in children: A randomized, controlled, prospective pilot study
Authors: Bar‐Yoseph Ronen , Kugelman Nir , Livnat Galit , Gur Michal , Hakim Fahed , Nir Vered , Bentur Lea 
Published: October 2016

SummaryBackground and ObjectivesAsthma is a chronic inflammatory disorder requiring intermittent or continuous anti‐inflammatory therapy. Patients often turn to alternative treatments as complements or replacements to conventional treatments. We aimed to evaluate the effect of salt room chambers (halotherapy) on bronchial hyper‐responsiveness (BHR), fractional exhaled nitric oxide (FeNO), and quality of life in children with asthma.Patients and MethodsChildren aged 5–13 years with a clinical diagnosis of mild asthma not receiving anti‐inflammatory therapy. Patients were randomized in this double‐blind, controlled study to salt room with halogenerator (treatment group), or without halogenerator (control group). We evaluated the effect of salt room therapy on BHR, FeNO, spirometry, and pediatric asthma quality of life questionnaire (PAQLQ). The treatment period lasted 7 weeks, 14 sessions.ResultsTwenty‐nine patients were randomized to the salt room with halogenerator (treatment group), and 26 patients to the salt room without salt halogenerator (control group). A statistically significant improvement in BHR was demonstrated in the treatment group, which remained unchanged in the control group. There was no improvement in spirometry or FeNO levels following treatment. The treatment group showed a statistical improvement in most parameters of quality of life questionnaires.ConclusionsOur pilot study suggests that salt room with halogenerator, may have some beneficial effects in mild asthmatic children. Randomized and larger controlled trials with long‐term follow‐up are necessary. Pediatr Pulmonol. 2017;52:580–587. © 2016 Wiley Periodicals, Inc.
Recent Developments for Prediction of Power of Aromatic and Non‐Aromatic Energetic Materials along with a Novel Computer Code for Prediction of Their Power
Authors: Keshavarz Mohammad Hossein , Azarniamehraban Jamshid , Atabak Hamidreza Hafizi , Ferdowsi Mohammad 
Published: October 2016

AbstractThe explosive power or strength of an energetic material shows its capacity for doing useful work. This work reviews recent developments for prediction of power of energetic compounds. A new user‐friendly computer code is also introduced to predict the relative power of a desired energetic compound as compared to 2,4,6‐trinitrotoluene (TNT). It is based on the best available methods, which can be used for different types of energetic compounds including nitroaromatics, nitroaliphatics, nitramines, and nitrate esters. The computed relative powers are consistent with the measured data for some new materials containing complex molecular structures.
Activation of UPR Signaling Pathway is Associated With the Malignant Progression and Poor Prognosis in Prostate Cancer
Authors: Liu Junnan , Xiao Maolin , Li Jianjun , Wang Delin , He Yunfeng , He Jiang , Gao Fei , Mai Li , Li Ying , Liang Yong , Liu Yuejiang , Zhong Xiaoni 
Published: October 2016

BACKGROUNDCurrently, the role of UPR signaling in prostate cancer (PCa) is unclear. To evaluate the relationship between UPR signaling pathway and the prognosis of PCa, we explored the expression of IRE1, PERK, and ATF6 in tissues.METHODSA total of 160 PCa and 30 benign prostate hyperplasia (BPH) tissues were collected. The expression of UPR signaling factors was assessed by immunohistochemistry. The staining characteristics were identified and evaluated for associations with clinicopathologic parameters, PSA recurrence survival, and prostate cancer‐specific morality.RESULTSThe expressions of ATF6α, PERK, and IRE1α were significantly associated with Gleason grade, PSA level, T stages and M stage, while this association was not significant in N stage. Additionally, UPR signaling factors expressed correlatively with each other. In further studies, high expression level of UPR signaling factors was usually detected in patients who suffered poor prognosis. Patients with positive UPR signaling factors meet shorter survival duration both on cancer‐specific morality and PSA recurrence. Multivariate analysis showed that IRE1α (HR = 4.461 95%CI = 1.270–15.670 P = 0.020) could be a potential factor in predicting PSA recurrence independently.CONCLUSIONSUPR signaling factors were co‐activated and activation of UPR signaling was implicated to the malignant progression and worse prognosis of PCa. The mechanism and function of UPR signaling in PCa are still to be determined. Prostate 77:274–281, 2017. © 2016 Wiley Periodicals, Inc.
Tailoring Pore Structure of Ultralight Electrospun Sponges by Solid Templating
Authors: Deuber Fabian , Mousavi Sara , Hofer Marco , Adlhart Christian 
Published: October 2016

AbstractFreeze‐casted nanofiber based sponges or aerogels exhibit a hierarchical porous structure. Pore formation is only partially understood. Therefore, we studied the underlying solid templating mechanism. We were able to tailor the secondary pore size between 9.5 and 123 μm while retaining the smaller primary pores known from electrospun nanofiber membranes. To understand the effect of microstructure on the sponges’ bulk properties, mass flow through the pores and interaction with the sponges’ internal surface were investigated. By solely altering the sponges’ microstructure we indeed found tunability in permeability by a factor 7 and in filtration efficiency by a factor of 220. Hence, pore architecture of nanofiber based sponges is a key element for their performance. The selected pullulan/PVA polymer blends and aqueous electrospinning conditions are benign and allow the facile adaptation of these ultralight highly porous sponges for a large number of applications.
A simplification of a real‐time verification problem
Authors: Roy Suman , Misra Janardan , Saha Indranil 
Published: October 2016

SummaryWe revisit the problem of real‐time verification with dense‐time dynamics using timeout and calendar‐based models and simplify this to a finite state verification problem. We introduce a specification formalism for these models and capture their behaviour in terms of semantics of timed transition systems. We discuss a technique, which reduces the problem of verification of qualitative temporal properties on infinite state space of a large fragment of these timeout and calender‐based transition systems into that on clock‐less finite state models through a two‐step process comprising of digitization and finitary reduction. This technique enables us to verify safety invariants for real‐time systems using finite state model checking avoiding the complexity of infinite state (bounded) model checking and scale up models without applying techniques from induction‐based proof methodology. In the same manner, we verify timeliness properties. Moreover, we can verify liveness for real‐time systems, which are not possible by using induction with infinite state model checkers. Copyright © 2016 John Wiley & Sons, Ltd.
Global Employment and Social Trends
Authors:   Research Department
Published: October 2016

The global economy is showing new signs of weakness…The world economy is estimated to have expanded by 3.1 per cent in 2015, over half a percentage point less than had been projected a year earlier. If current policy responses are maintained, the outlook is for continued economic weakening, posing significant challenges to enterprises and workers. Indeed, over the next two years, the world economy is projected to grow by only around 3 per cent, significantly less than before the advent of the global crisis.The continuing slowdown in economic growth is being driven by weakness in emerging and developing countries. China is facing a pronounced slowdown. This, combined with other factors, has contributed to a steep decline in commodity prices, particularly those related to energy. This situation has, in turn, affected large emerging economy commodity exporters, such as Brazil and the Russian Federation, which have entered a period of recession. The benefits accruing to net commodity importers have been insufficient to offset the decline affecting exporters. Another sign of economic weakness is the fact that global trade, which had typically expanded twice as fast as the global economy, is now growing in line with or at a lower rate than global growth.
Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3
Authors: Magliarelli Helena de Fatima , Matondo Mariette , Mészáros Gergő , Goginashvili Alexander , Erbs Eric , Zhang Zhirong , Mihlan Michael , Wolfrum Christian , Aebersold Ruedi , Sumara Izabela , Ricci Romeo 
Published: October 2016

AbstractAdaptation to changes in nutrient availability is crucial for cells and organisms. Posttranslational modifications of signaling proteins are very dynamic and are therefore key to promptly respond to nutrient deprivation or overload. Herein we screened for ubiquitylation of proteins in the livers of fasted and refed mice using a comprehensive systemic proteomic approach. Among 1641 identified proteins, 117 were differentially ubiquitylated upon fasting or refeeding. Endoplasmic reticulum (ER) and secretory proteins were enriched in the livers of refed mice in part owing to an ER-stress-mediated response engaging retro-translocation and ubiquitylation of proteins from the ER. Complement C3, an innate immune factor, emerged as the most prominent ER-related hit of our screen. Accordingly, we found that secretion of C3 from the liver and primary hepatocytes as well as its dynamic trafficking are nutrient dependent. Finally, obese mice with a chronic nutrient overload show constitutive trafficking of C3 in the livers despite acute changes in nutrition, which goes in line with increased C3 levels and low-grade inflammation reported for obese patients. Our study thus suggests that nutrient sensing in the liver is coupled to release of C3 and potentially its metabolic and inflammatory functions.
DDX3 DEAD-box RNA helicase plays a central role in mitochondrial protein quality control in Leishmania
Authors: Padmanabhan Prasad Kottayil , Zghidi-Abouzid Ouafa , Samant Mukesh , Dumas Carole , Aguiar Bruno Guedes , Estaquier Jerome , Papadopoulou Barbara 
Published: October 2016

AbstractDDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoan Leishmania. We show that genetic inactivation of DDX3 leads to the accumulation of mitochondrial reactive oxygen species (ROS) associated with a defect in hydrogen peroxide detoxification. Upon stress, ROS production is greatly enhanced, causing mitochondrial membrane potential loss, mitochondrial fragmentation, and cell death. Importantly, this phenotype is exacerbated upon oxidative stress in parasites forced to use the mitochondrial oxidative respiratory machinery. Furthermore, we show that in the absence of DDX3, levels of major components of the unfolded protein response as well as of polyubiquitinated proteins increase in the parasite, particularly in the mitochondrion, as an indicator of mitochondrial protein damage. Consistent with these findings, immunoprecipitation and mass-spectrometry studies revealed potential interactions of DDX3 with key components of the cellular stress response, particularly the antioxidant response, the unfolded protein response, and the AAA-ATPase p97/VCP/Cdc48, which is essential in mitochondrial protein quality control by driving proteosomal degradation of polyubiquitinated proteins. Complementation studies using DDX3 deletion mutants lacking conserved motifs within the helicase core support that binding of DDX3 to ATP is essential for DDX3’s function in mitochondrial proteostasis. As a result of the inability of DDX3-depleted Leishmania to recover from ROS damage and to survive various stresses in the host macrophage, parasite intracellular development was impaired. Collectively, these observations support a central role for the Leishmania DDX3 homolog in preventing ROS-mediated damage and in maintaining mitochondrial protein quality control.
Simple phosphinate ligands access zinc clusters identified in the synthesis of zinc oxide nanoparticles
Authors: Pike Sebastian D. , White Edward R. , Shaffer Milo S. P. , Williams Charlotte K. 
Published: October 2016

AbstractThe bottom-up synthesis of ligand-stabilized functional nanoparticles from molecular precursors is widely applied but is difficult to study mechanistically. Here we use31P NMR spectroscopy to follow the trajectory of phosphinate ligands during the synthesis of a range of ligated zinc oxo clusters, containing 4, 6 and 11 zinc atoms. Using an organometallic route, the clusters interconvert rapidly and self-assemble in solution based on thermodynamic equilibria rather than nucleation kinetics. These clusters are also identifiedin situduring the synthesis of phosphinate-capped zinc oxide nanoparticles. Unexpectedly, the ligand is sequestered to a stable Zn11cluster during the majority of the synthesis and only becomes coordinated to the nanoparticle surface, in the final step. In addition to a versatile and accessible route to (optionally doped) zinc clusters, the findings provide an understanding of the role of well-defined molecular precursors during the synthesis of small (2–4 nm) nanoparticles.
Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
Authors: Brown Amanda M. V. , Wasala Sulochana K. , Howe Dana K. , Peetz Amy B. , Zasada Inga A. , Denver Dee R. 
Published: October 2016

AbstractWolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nematode Pratylenchus penetrans. Phylogenomic analyses placed wPpe as the earliest diverging Wolbachia, suggesting two evolutionary invasions into nematodes. The next branches comprised strains in sap-feeding insects, suggesting Wolbachia may have first evolved as a nutritional mutualist. Genome size, protein content, %GC, and repetitive DNA allied wPpe with mutualistic Wolbachia, whereas gene repertoire analyses placed it between parasite (A, B) and mutualist (C, D, F) groups. Conservation of iron metabolism genes across Wolbachia suggests iron homeostasis as a potential factor in its success. This study enhances our understanding of this globally pandemic endosymbiont, highlighting genetic patterns associated with host changes. Combined with future work on this strain, these genomic data could help provide potential new targets for plant-parasitic nematode control.
Quadrupole lattice resonances in plasmonic crystal excited by cylindrical vector beams
Authors: Sakai Kyosuke , Nomura Kensuke , Yamamoto Takeaki , Omura Tatsuya , Sasaki Keiji 
Published: October 2016

AbstractWe report a scheme to exploit low radiative loss plasmonic resonance by combining a dark (subradiant) mode and a lattice resonance. We theoretically demonstrate that such dark-mode lattice resonances in periodic arrays of nanodisks or plasmonic crystals can be excited by vertically incident light beams. We investigate the excitation of lattice resonances in a finite sized, square-lattice plasmonic crystal by two types of cylindrical vector beams and a linearly polarized Gaussian beam. Quadrupole lattice resonances are excited by all three beams, and the largest peak intensity is obtained by using a specific type of cylindrical vector beam. Because of their lower radiative losses with many hotspots, the quadrupole lattice resonances in plasmonic crystal may pave the way for photonic research and applications that require strong light-matter interactions.
Quantum key distribution with prepare-and-measure Bell test
Authors: Tan Yong-gang 
Published: October 2016

AbstractThe prepare-and-measure quantum key distribution (QKD) has the merits of fast speed, high key generation rate, and easy implementation. However, the detector side channel attacks greatly undermine the security of the key bits. The eavesdropper, Eve, exploits the flaws of the detectors to obtain illegal information without violating quantum principles. It means that she can intervene in the communication without being detected. A prepare-and-measure Bell test protocol will be proposed. By randomly carrying out Bell test at the side of the information receiver, Bob, Eve’s illegal information gain within the detector side channel attack can be well bounded. This protocol does not require any improvement on the detectors used in available prepare-and-measure QKD. Though we only illustrate its application in the BB84 protocol, it is applicable for any prepare-and-measure QKD.
Graphlet-based Characterization of Directed Networks
Authors: Sarajlić Anida , Malod-Dognin Noël , Yaveroğlu Ömer Nebil , Pržulj Nataša 
Published: October 2016

AbstractWe are flooded with large-scale, dynamic, directed, networked data. Analyses requiring exact comparisons between networks are computationally intractable, so new methodologies are sought. To analyse directed networks, we extend graphlets (small induced sub-graphs) and their degrees to directed data. Using these directed graphlets, we generalise state-of-the-art network distance measures (RGF, GDDA and GCD) to directed networks and show their superiority for comparing directed networks. Also, we extend the canonical correlation analysis framework that enables uncovering the relationships between the wiring patterns around nodes in a directed network and their expert annotations. On directed World Trade Networks (WTNs), our methodology allows uncovering the core-broker-periphery structure of the WTN, predicting the economic attributes of a country, such as its gross domestic product, from its wiring patterns in the WTN for up-to ten years in the future. It does so by enabling us to track the dynamics of a country’s positioning in the WTN over years. On directed metabolic networks, our framework yields insights into preservation of enzyme function from the network wiring patterns rather than from sequence data. Overall, our methodology enables advanced analyses of directed networked data from any area of science, allowing domain-specific interpretation of a directed network’s topology.
Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression – implications for atherosclerosis research
Authors: Bisgaard Line S. , Mogensen Christina K. , Rosendahl Alexander , Cucak Helena , Nielsen Lars Bo , Rasmussen Salka E. , Pedersen Tanja X. 
Published: October 2016

AbstractMacrophages are heterogeneous and can polarize into specific subsets, e.g. pro-inflammatory M1-like and re-modelling M2-like macrophages. To determine if peritoneal macrophages (PEMs) or bone marrow derived macrophages (BMDMs) resembled aortic macrophages from ApoE−/− mice, their M1/M2 phenotype, inflammatory status, and lipid metabolism signatures were compared. oxLDL accumulation was similar in PEMs and BMDMs. On protein expression level, BMDMs showed an M2-like CD206highCD11clow profile, while cholesterol loading led to enhanced CD11c expression and reduced MCP-1 secretion. In contrast, PEMs expressed low levels of CD206 and CD11c, and responded to cholesterol loading by increasing CD11c expression and MCP-1 secretion. mRNA expression of M1/M2 markers was higher in PEMS than BMDMs, while lipid metabolism genes were similarly expressed. Whole aorta flow cytometry showed an accumulation of M2-like CD206highCD11clow macrophages in advanced versus early atherosclerotic disease in ApoE−/− mice. In isolated lesions, mRNA levels of the M2 markers Socs2, CD206, Retnla, and IL4 were downregulated with increasing disease severity. Likewise, mRNA expression of lipid metabolism genes (SREBP2, ACSL1, SRB1, DGAT1, and cpt1a) was decreased in advanced versus early lesions. In conclusion, PEMs and BMDMs are phenotypically distinct and differ from macrophages in lesions with respect to expression of M1/M2 markers and lipid metabolism genes.
The Role of PIK3CA Mutations among Lung Adenocarcinoma Patients with Primary and Acquired Resistance to EGFR Tyrosine Kinase Inhibition
Authors: Wu Shang-Gin , Chang Yih-Leong , Yu Chong-Jen , Yang Pan-Chyr , Shih Jin-Yuan 
Published: October 2016

AbstractTo understand the impact of PIK3CA mutations on clinical characteristics and treatment response to epidermal growth factor tyrosine kinase inhibitors (EGFR TKIs) of lung adenocarcinoma, we examined PIK3CA and EGFR mutations in lung adenocarcinoma patients, and analyzed their clinical outcomes. Surgically excised tumor, bronchoscopy biopsy/brushing specimens and pleural effusions were prospectively collected from 1029 patients. PIK3CA and EGFR mutations were analyzed by RT-PCR and direct sequencing. In EGFR TKI-nave specimens, PIK3CA mutation rate was 1.8% (14/760). Twelve patients had coexisting PIK3CA and EGFR mutations. Among the 344 EGFR TKI-treated EGFR mutant patients, there was no significant difference in treatment response (p = 0.476) and progression-free survival (p = 0.401) of EGFR TKI between PIK3CA mutation-positive and negative patients. The PIK3CA mutation rate in lung adenocarcinoma with acquired resistance to EGFR TKI is not higher than that in EGFR TKI-naïve tissue specimens (2.9% (6/207) vs. 1.8%; p = 0.344). Of the 74 patients with paired specimens (TKI-naïve and acquired resistance to TKIs) only one patient (1.4%) developed acquired PIK3CA (E545K) mutation, and he also had acquired EGFR (T790M) mutation. In conclusion, PIK3CA mutation may not be associated with primary resistance to EGFR TKI among lung adenocarcinoma patients. Acquired PIK3CA mutation related to EGFR TKI treatment is rare.
Metabolomics approach reveals metabolic disorders and potential biomarkers associated with the developmental toxicity of tetrabromobisphenol A and tetrachlorobisphenol A
Authors: Ye Guozhu , Chen Yajie , Wang Hong-ou , Ye Ting , Lin Yi , Huang Qiansheng , Chi Yulang , Dong Sijun 
Published: October 2016

AbstractTetrabromobisphenol A and tetrachlorobisphenol A are halogenated bisphenol A (H-BPA), and has raised concerns about their adverse effects on the development of fetuses and infants, however, the molecular mechanisms are unclear, and related metabolomics studies are limited. Accordingly, a metabolomics study based on gas chromatography-mass spectrometry was employed to elucidate the molecular developmental toxicology of H-BPA using the marine medaka (Oryzias melastigmas) embryo model. Here, we revealed decreased synthesis of nucleosides, amino acids and lipids, and disruptions in the TCA (tricarboxylic acid) cycle, glycolysis and lipid metabolism, thus inhibiting the developmental processes of embryos exposed to H-BPA. Unexpectedly, we observed enhanced neural activity accompanied by lactate accumulation and accelerated heart rates due to an increase in dopamine pathway and a decrease in inhibitory neurotransmitters following H-BPA exposure. Notably, disorders of the neural system, and disruptions in glycolysis, the TCA cycle, nucleoside metabolism, lipid metabolism, glutamate and aspartate metabolism induced by H-BPA exposure were heritable. Furthermore, lactate and dopa were identified as potential biomarkers of the developmental toxicity of H-BPA and related genetic effects. This study has demonstrated that the metabolomics approach is a useful tool for obtaining comprehensive and novel insights into the molecular developmental toxicity of environmental pollutants.