bluuebunny/crossref_metadata_2025_split huggingface dataset:
Type IX secretion: the generation of bacterial cell surface coatings involved in virulence, gliding motility and the degradation of complex biopolymers
Authors: Veith Paul D. , Glew Michelle D. , Gorasia Dhana G. , Reynolds Eric C. 
Published: July 2017

SummaryThe Type IX secretion system (T9SS) is present in over 1000 sequenced species/strains of the Fibrobacteres‐Chlorobi‐Bacteroidetes superphylum. Proteins secreted by the T9SS have an N‐terminal signal peptide for translocation across the inner membrane via the SEC translocon and a C‐terminal signal for secretion across the outer membrane via the T9SS. Nineteen protein components of the T9SS have been identified including three, SigP, PorX and PorY that are involved in regulation. The inner membrane proteins PorL and PorM and the outer membrane proteins PorK and PorN interact and a complex comprising PorK and PorN forms a large ring structure of 50 nm in diameter. PorU, PorV, PorQ and PorZ form an attachment complex on the cell surface of the oral pathogen, Porphyromonas gingivalis. P. gingivalis T9SS substrates bind to PorV suggesting that after translocation PorV functions as a shuttle protein to deliver T9SS substrates to the attachment complex. The PorU component of the attachment complex is a novel Gram negative sortase which catalyses the cleavage of the C‐terminal signal and conjugation of the protein substrates to lipopolysaccharide, anchoring them to the cell surface. This review presents an overview of the T9SS focusing on the function of T9SS substrates and machinery components.
Anti‐feeding and insecticidal efficacy of a topical administration of dinotefuran–pyriproxyfen–permethrin spot‐on (Vectra® 3D) on mice against Stegomyia albopicta (= Aedes albopictus)
Authors: TAHIR D. , DAVOUST B. , ALMERAS L. , BERENGER J. M. , VARLOUD M. , PAROLA P. 
Published: July 2017

AbstractAn ectoparasiticide combining three active ingredients [dinotefuran, permethrin and pyriproxyfen (DPP)] was used in mice in an experiment designed to evaluate its anti‐feeding and insecticidal efficacy against Stegomyia albopicta (= Aedes albopictus) (Diptera: Culicidae) mosquitoes. Twenty‐two adult mice were randomly allocated into two groups consisting of an untreated control group and a DPP‐treated group. Mice were exposed individually for 1 h to a mean ± standard deviation of 27 ± 2 starved female mosquitoes on days 1, 7, 14, 21 and 28 post‐treatment. At the end of the exposure (1 h), mosquitoes were assessed for immediate survival and engorgement status. Additionally, live mosquitoes in both groups were incubated separately and observed for mortality at 24 h after the end of the exposure. The anti‐feeding efficacy of DPP after the 1‐h exposure period was 99.2, 100, 98.0, 89.3 and 87.4% at 1, 7, 14, 21 and 28 days, respectively. Levels of insecticidal efficacy evaluated at 1 h and 24 h after exposure on days 1, 7, 14, 21 and 28 were 36.7, 28.9, 30.8, 23.1 and 11.9%, and 68.4, 45.0, 43.3, 37.9 and 19.9%, respectively. Based on the mouse model, the present study demonstrates that the DPP combination has significant anti‐feeding and insecticidal efficacy against S. albopicta for at least 4 weeks.
Seasonal dynamics of the tick Haemaphysalis tibetensis in the Tibetan Plateau, China
Authors: LIU M. , LI T. , YU Z. J. , QIU Z. X. , YAN P. , LI Y. , LIU J. 
Published: July 2017

AbstractThe tick Haemaphysalis tibetensis (Acari: Ixodidae) Hoogstraal is an important arthropod vector widespread in the Qinghai‐Tibet Plateau, and knowledge of its seasonal dynamics is still poor. The current study investigated the seasonal dynamics of the parasitic and non‐parasitic H. tibetensis over a 2‐year period from March 2014 to February 2016 in the Tibetan Plateau, China. During this timeframe, non‐parasitic ticks were collected weekly by flag‐dragging in grassland and shrubland areas, and parasitic ticks were removed weekly from selected sheep. Plateau pikas were captured using traps and examined for immature ticks between May to September 2014. Results suggest that non‐parasitic H. tibetensis were mainly distributed in the grassland, and the parasitic adults and nymphs were found mostly on sheep. Larvae were usually found on Plateau pikas and the prevalence of infestation and mean parasitic intensity were 72.1 and 1.81%, respectively. Adults were observed from March to July with the major peak occurring in mid‐April. Nymphs were found from March to August and reached a peak in late June. Larvae were collected from April to September, and their numbers peaked in late May. In the parasitic and non‐parasitic period, the overall sex ratio of males to females was 1.62 and 1.30, respectively. Results show that H. tibetensis can complete one generation per year, with a population overlap between stages over the spring‐summer months. These findings provide additional information on the biology and ecology of H. tibetensis as well as insights on its control in the environment and on sheep.
The Best Predictors of Survival: Do They Vary by Age, Sex, and Race?
Authors: Goldman Noreen , Glei Dana A. , Weinstein Maxine 
Published: July 2017

AbstractWe consider a broad set of variables used by social scientists and clinicians to identify the leading predictors of five‐year survival among American adults. We address a question not considered in earlier research: Do the strongest predictors of survival vary by age, sex or race/ethnicity? The analysis uses hazard models with 30 well‐established predictors to examine five‐year survival in the National Health and Nutrition Examination Survey. We find that the simple measure of self‐assessed health and self‐reported measures of functional ability and disability are the strongest predictors in all demographic groups, and are generally ranked considerably higher than biomarkers. Among the biomarkers, serum albumin is highly ranked in most demographic groups, whereas clinical measures of cardiovascular and metabolic function are consistently among the weakest predictors. Despite these similarities, there is substantial variation in the leading predictors across demographic groups, most notably by race and ethnicity
Comparative experimental taphonomy of eight marine arthropods indicates distinct differences in preservation potential
Authors: Klompmaker Adiël A. , Portell Roger W. , Frick Michael G. 
Published: July 2017

AbstractGlobal biodiversity patterns in deep time can only be understood fully when the relative preservation potential of each clade is known. The relative preservation potential of marine arthropod clades, a diverse and ecologically important component of modern and past ecosystems, is poorly known. We tackled this issue by carrying out a 205‐day long comprehensive, comparative, taphonomic experiment in a laboratory by scoring up to ten taphonomic characters for multiple specimens of seven crustacean and one chelicerate species (two true crabs, one shrimp, one lobster, one hermit crab, one stomatopod, one barnacle and one horseshoe crab). Although the results are preliminary because we used a single experimental setup and algal growth partially hampered observations, some parts of hermit crabs, stomatopods, swimming crabs and barnacles decayed slowly relative to other parts, implying differential preservation potentials within species, largely consistent with the fossil record of these groups. An inferred parasitic isopod, manifested by a bopyriform swelling within a hermit crab carapace, decayed relatively fast. We found limited variation in the decay rate between conspecifics, and we did not observe size‐related trends in decay rate. Conversely, substantial differences in the decay rate between species were seen after c. 50 days, with shrimps and stomatopods decaying fastest, suggesting a relatively low preservation potential, whereas the lobster, calico crabs, horseshoe crabs and barnacles showed relatively slow decay rates, suggesting a higher preservation potential. These results are supported by two modern and fossil record‐based preservation potential metrics that are significantly correlated to decay rate ranks. Furthermore, we speculate that stemward slippage may not be ubiquitous in marine arthropods. Our results imply that diversity studies of true crabs, lobsters, horseshoe crabs and barnacles are more likely to yield patterns that are closer to their true biodiversity patterns than those for stomatopods, shrimps and hermit crabs.
Membrane topology and identification of key residues of EaDAcT, a plant MBOAT with unusual substrate specificity
Authors: Tran Tam N. T. , Shelton Jennifer , Brown Susan , Durrett Timothy P. 
Published: July 2017

SummaryEuonymus alatus diacylglycerol acetyltransferase (EaDAcT) catalyzes the transfer of an acetyl group from acetyl‐CoA to the sn‐3 position of diacylglycerol to form 3‐acetyl‐1,2‐diacyl‐sn‐glycerol (acetyl‐TAG). EaDAcT belongs to a small, plant‐specific subfamily of the membrane bound O‐acyltransferases (MBOAT) that acylate different lipid substrates. Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the same fractions as an endoplasmic reticulum (ER)‐specific marker. By mapping the membrane topology of EaDAcT, we obtained an experimentally determined topology model for a plant MBOAT. The EaDAcT model contains four transmembrane domains (TMDs), with both the N‐ and C‐termini orientated toward the lumen of the ER. In addition, there is a large cytoplasmic loop between the first and second TMDs, with the MBOAT signature region of the protein embedded in the third TMD close to the interface between the membrane and the cytoplasm. During topology mapping, we discovered two cysteine residues (C187 and C293) located on opposite sides of the membrane that are important for enzyme activity. In order to identify additional amino acid residues important for acetyltransferase activity, we isolated and characterized acetyltransferases from other acetyl‐TAG‐producing plants. Among them, the acetyltransferase from Euonymus fortunei possessed the highest activity in vivo and in vitro. Mutagenesis of conserved amino acids revealed that S253, H257, D258 and V263 are essential for EaDAcT activity. Alteration of residues unique to the acetyltransferases did not alter the unique acyl donor specificity of EaDAcT, suggesting that multiple amino acids are important for substrate recognition.
Implementing a protocol to optimize blood use in a cardiac surgery service: results of a pre‐post analysis and the impact of high‐volume blood users
Authors: Grau Juan B. , Fortier Jacqueline H. , Kuschner Cyrus , Ferrari Giovanni , Brizzio Mariano E. , Zapolanski Alex , Shaw Richard E. 
Published: July 2017

BACKGROUNDBlood transfusions are a common and costly intervention for cardiac surgery patients. Evidence suggests that a more restrictive transfusion strategy may reduce costs and transfusion‐related complications without increasing perioperative morbidity and mortality.STUDY DESIGN AND METHODSA transfusion‐limiting protocol was developed and implemented in a cardiovascular surgery unit. Over a 5‐year period, data were collected on patient characteristics, procedures, utilization of blood products, morbidity, and mortality, and these were compared before and after the protocol was implemented.RESULTSAfter the protocol was put in place, fewer patients required transfusions (38.2% vs. 45.5%, p = 0.004), with the greatest reduction observed in postoperative blood use (29.1% vs. 37.2%, p = 0.001). In‐hospital morbidity and mortality did not increase. When patients who received transfusions were stratified by procedure, the protocol was most effective in reducing transfusions for patients undergoing isolated coronary artery bypass grafting (CABG; 4.09 units vs. 2.51 units, p = 0.009) and CABG plus valve surgery (10.32 units vs. 4.77 units, p = 0.014). A small group of patients were disproportionate recipients of transfusions, with approximately 6% of all patients receiving approximately half of the blood products.CONCLUSIONA protocol to limit transfusions decreased the proportion of cardiothoracic surgery patients who received blood products. A very small group of patients received a large number of transfusions, and within that group the observed mortality was significantly higher than in the general patient population. Current protocols cannot possibly account for these patients, and this should be considered when analyzing the performance of protocols designed to reduce unnecessary transfusions.
High hepatitis E virus antibody positive rates in dogs and humans exposed to dogs in the south‐west of China
Authors: Zeng M. Y. , Gao H. , Yan X. X. , Qu W. J. , Sun Y. K. , Fu G. W. , Yan Y. L. 
Published: July 2017

SummaryHepatitis E (HE) is a zoonotic viral disease caused by hepatitis E virus (HEV). The objective of this study was to investigate the prevalence of HEV infection among dogs and humans exposed to dogs in the south‐west region of China. A total of 4,490 dog serum samples and 2,206 relative practitioner serum samples were collected from 18 pet hospitals and dog farms in Yunnan, Sichuan and Guizhou province, and the anti‐HEV IgG antibodies were detected by ELISA. The results showed that the total positive rate of anti‐HEV antibodies was 36.55% with the highest rate in city stray dogs, and the differences in distinct species and growth phases were significant. The positive rate of anti‐HEV antibody in veterinarian and farm staff‐related practitioners was significantly higher than the general population. The finding of the present survey suggested that high HEV seroprevalence in dogs and humans exposed to dogs in the south‐west area of China poses a significant public health concern. It is urgent to improve integrated strategies to detect, prevent and control HEV infection in dogs and humans exposed to dogs in this area.
Acetylcholine‐dependent upregulation of TASK‐1 channels in thalamic interneurons by a smooth muscle‐like signalling pathway
Authors: Leist Michael , Rinné Susanne , Datunashvili Maia , Aissaoui Ania , Pape Hans‐Christian , Decher Niels , Meuth Sven G. , Budde Thomas 
Published: July 2017

Key points
The ascending brainstem transmitter acetylcholine depolarizes thalamocortical relay neurons while it induces hyperpolarization in local circuit inhibitory interneurons.
Sustained K+ currents are modulated in thalamic neurons to control their activity modes; for the interneurons the molecular nature of the underlying ion channels is as yet unknown.
Activation of TASK‐1 K+ channels results in hyperpolarization of interneurons and suppression of their action potential firing.
The modulation cascade involves a non‐receptor tyrosine kinase, c‐Src.
The present study identifies a novel pathway for the activation of TASK‐1 channels in CNS neurons that resembles cholinergic signalling and TASK‐1 current modulation during hypoxia in smooth muscle cells.
AbstractThe dorsal part of the lateral geniculate nucleus (dLGN) is the main thalamic site for state‐dependent transmission of visual information. Non‐retinal inputs from the ascending arousal system and inhibition provided by γ‐aminobutyric acid (GABA)ergic local circuit interneurons (INs) control neuronal activity within the dLGN. In particular, acetylcholine (ACh) depolarizes thalamocortical relay neurons by inhibiting two‐pore domain potassium (K2P) channels. Conversely, ACh also hyperpolarizes INs via an as‐yet‐unknown mechanism. By using whole cell patch‐clamp recordings in brain slices and appropriate pharmacological tools we here report that stimulation of type 2 muscarinic ACh receptors induces IN hyperpolarization by recruiting the G‐protein βγ subunit (Gβγ), class‐1A phosphatidylinositol‐4,5‐bisphosphate 3‐kinase, and cellular and sarcoma (c‐Src) tyrosine kinase, leading to activation of two‐pore domain weakly inwardly rectifying K+ channel (TWIK)‐related acid‐sensitive K+ (TASK)‐1 channels. The latter was confirmed by the use of TASK‐1‐deficient mice. Furthermore inhibition of phospholipase Cβ as well as an increase in the intracellular level of phosphatidylinositol‐3,4,5‐trisphosphate facilitated the muscarinic effect. Our results have uncovered a previously unknown role of c‐Src tyrosine kinase in regulating IN function in the brain and identified a novel mechanism by which TASK‐1 channels are activated in neurons.
A reduction in SK channels contributes to increased activity of hypothalamic magnocellular neurons during heart failure
Authors: Ferreira‐Neto Hildebrando C. , Biancardi Vinicia C. , Stern Javier E. 
Published: July 2017

Key points
Small conductance Ca2+‐activated K+ (SK) channels play an important role in regulating the excitability of magnocellular neurosecretory cells (MNCs). Although an increased SK channel function contributes to adaptive physiological responses, it remains unknown whether changes in SK channel function/expression contribute to exacerbated MNC activity under disease conditions.
We show that the input–output function of MNCs in heart failure (HF) rats is enhanced. Moreover, the SK channel blocker apamin enhanced the input–output function in sham, although not in HF rats.
We found that both the after‐hyperpolarizing potential magnitude and the underlying apamin‐sensitive IAHP are blunted in MNCs from HF rats.
The magnitude of spike‐induced increases in intracellular Ca2+ levels was not affected in MNCs of HF rats.
We found a diminished expression of SK2/SK3 channel subunit mRNA expression in the supraoptic nucleus of HF rats.
Our studies suggest that a reduction in SK channel expression, but not changes in Ca2+‐mediated activation of SK channels, contributes to exacerbated MNC activity in HF rats.
AbstractSmall conductance Ca2+‐activated K+ channels (SK) play an important role in regulating the activity of magnocellular neurosecretory cells (MNCs) and hormone release from the posterior pituitary. Moreover, enhanced SK activity contributes to the adaptive responses of MNCs to physiological challenge, such as lactation. Nevertheless, whether changes in SK function/expression contribute to exacerbated MNC activity during diseases such as heart failure (HF) remains unknown. In the present study, we used a combination of patch clamp electrophysiology, confocal Ca2+ imaging and molecular biology in a rat model of ischaemic HF. We found that the input–output function of MNCs was enhanced in HF compared to sham rats. Moreover, although the SK blocker apamin (200 nm) strengthened the input–output function in sham rats, it failed to have an effect in HF rats. The magnitude of the after‐hyperpolarizing potential (AHP) following a train of spikes and the underlying apamin‐sensitive IAHP were blunted in MNCs from HF rats. However, spike‐induced increases in intracellular Ca2+ were not affected in the MNCs of HF rats. Real‐time PCR measurements of SK channel subunits mRNA in supraoptic nucleus punches revealed a diminished expression of SK2/SK3 subunits in HF compared to sham rats. Together, our studies demonstrate that MNCs from HF rats exhibit increased membrane excitability and an enhanced input–output function, and also that a reduction in SK channel‐mediated, apamin‐sensitive AHP is a critical contributing mechanism. Moreover, our results suggest that the reduced AHP is related to a down‐regulation of SK2/SK3 channel subunit expression but not the result of a blunted activity‐dependent intracellular Ca2+ increase following a burst of action potentials.
Marathon Pace Control in Masters Athletes
Authors: Breen Derek , Norris Michelle , Healy Robin , Anderson Ross 
Published: July 2017

Purpose: Pacing strategies are key to overall performance outcome in distance-running events. Presently, no literature has examined pacing strategies used by masters athletes of all running levels during a competitive marathon. Therefore, this study aimed to examine masters athletes’ pacing strategies, categorized by gender, age, and performance level. Methods: Data were retrieved from the 2015 TSC New York City Marathon for 31,762 masters athletes (20,019 men and 11,743 women). Seven performance-classification (PC) groupings were identified via comparison of overall completion time compared with current world records, appropriate to age and gender. Data were categorized via, age, gender, and performance level. Mean 5-km speed for the initial 40 km was calculated, and the fastest and slowest 5-km-split speeds were identified and expressed as a percentage faster or slower than mean speed. Pace range, calculated as the absolute sum of the fastest and slowest split percentages, was then analyzed. Results: Significant main effects were identified for age, gender, and performance level (P < .001), with performance level the most determining factor. Athletes in PC1 displayed the lowest pace range (14.19% ± 6.66%), and as the performance levels of athletes decreased, pace range increased linearly (PC2–PC7, 17.52% ± 9.14% to 36.42% ± 18.32%). A significant interaction effect was found for gender × performance (P < .001), with women showing a smaller pace range (−3.81%). Conclusions: High-performing masters athletes use more-controlled pacing strategies than their lower-ranked counterparts during a competitive marathon, independent of age and gender.
Comparison of Methods of Calculating Dynamic Strength Index
Authors: Comfort Paul , Thomas Christopher , Dos’Santos Thomas , Jones Paul A. , Suchomel Timothy J. , McMahon John J. 
Published: July 2017

Purpose: To determine the reliability and variability of the Dynamic Strength Index (DSI) calculated from squat-jump (SJ) vs countermovement-jump (CMJ) peak force (PF) and to compare DSI values between methods. Methods: Male youth soccer and rugby league players (N = 27; age 17.2 ± 0.7 y, height 173.9 ± 5.7 cm, body mass 71.1 ± 7.2 kg) performed 3 trials of the SJ, CMJ, and isometric midthigh pull (IMTP) on 2 separate days. DSI was calculated by dividing the PF during each jump by the IMTP PF. Results: DSI-SJ exhibited moderate (intraclass correlation coefficient [ICC] = .419) within-session reliability and high variability (percentage coefficient of variation [%CV] = 15.91) during session 1; however, this improved noticeably during session 2 (ICC = .948, %CV = 4.03). In contrast, DSI-CMJ showed nearly perfect within-session reliability (ICC = .920–.952) and low variability (%CV = 3.80–4.57) for both sessions. Moreover, DSI-SJ values demonstrated a small yet significant increase between sessions (P = .01, d = 0.37), whereas only a trivial and nonsignificant increase was observed for DSI-CMJ between sessions (P = .796, d = 0.07). Between-sessions reliability was very high for the DSI-SJ (ICC = .741) and nearly perfect for the DSI-CMJ (ICC = .924). There was no significant or meaningful difference (P = .261, d = 0.12) between DSI-SJ (0.82 ± 0.18) and DSI-CMJ (0.84 ± 0.15). Conclusions: Practitioners should use DSI-CMJ, as it is a more reliable measure than DSI-SJ, although it produces similar ratios.