bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA HOTTIP promotes BCL-2 expression and induces chemoresistance in small cell lung cancer by sponging miR-216a
Authors: Sun Yanqin , Hu Bingshuang , Wang Qiongyao , Ye Minting , Qiu Qianqian , Zhou Yuanyuan , Zeng Fanrui , Zhang Xiaomin , Guo Ying , Guo Linlang 
Published: January 2018

AbstractDespite progress in treatment of small cell lung cancer (SCLC), its multidrug chemoresistance and poor prognosis still remain. Recently, we globally assessed long non-coding RNAs (lncRNAs) for contributions to SCLC chemoresistance using microarray data, in vitro and in vivo assays. Here we reported that HOTTIP, encoding a lncRNA that is frequently amplified in SCLC, was associated with SCLC cell chemosensitivity, proliferation, and poor prognosis of SCLC patients. Moreover, mechanistic investigations showed that HOTTIP functioned as an oncogene in SCLC progression by binding miR-216a and abrogating its tumor-suppressive function in this setting. On the other hand, HOTTIP increased the expression of anti-apoptotic factor BCL-2, another important target gene of miR-216a, and jointly enhanced chemoresistance of SCLC by regulating BCL-2. Taken together, our study established a role for HOTTIP in SCLC progression and chemoresistance suggest its candidacy as a new diagnostic and prognostic biomarker for clinical management of SCLC.
Transmembrane protein 170B is a novel breast tumorigenesis suppressor gene that inhibits the Wnt/β-catenin pathway
Authors: Li Mengwei , Han Yanzhen , Zhou Haoze , Li Xin , Lin Chenyu , Zhang Erhao , Chi Xiaowei , Hu Jialiang , Xu Hanmei 
Published: January 2018

AbstractThe identification of specific drug targets guides the development of precise cancer treatments. Compared with oncogenes, tumor suppressor genes have been poorly studied in the treatment of breast cancer. We integrate the microRNA expression array from GEO (Gene Expression Omnibus) and TCGA (The Cancer Genome Atlas) databases in clinical breast cancer tissues, and find that miR-27a is significantly upregulated and correlated with poor survival outcome and tumor progression. Transmembrane protein 170B (TMEM170B), a new functional target of miR-27a, is identified via target prediction and experimental validation, suppressing breast cancer proliferation, metastasis, and tumorigenesis. Furthermore, TMEM170B overexpression promotes cytoplasmic β-catenin phosphorylation, resulting in the inhibition of β-catenin stabilization, reduction of nuclear β-catenin levels and downstream targets expression. Clinically, TMEM170B or β-catenin expression is significantly correlated with overall survival ratio in breast cancer patients. Thus, these results highlight TMEM170B as a novel tumor suppressor target in association with the β-catenin pathway, which may provide a new therapeutic approach for human breast cancer therapy.
Covalent ISG15 conjugation to CHIP promotes its ubiquitin E3 ligase activity and inhibits lung cancer cell growth in response to type I interferon
Authors: Yoo Lang , Yoon A-Rum , Yun Chae-Ok , Chung Kwang Chul 
Published: January 2018

AbstractThe carboxyl terminus of Hsp70-interacting protein (CHIP) acts as a ubiquitin E3 ligase and a link between the chaperones Hsp70/90 and the proteasome system, playing a vital role in maintaining protein homeostasis. CHIP regulates a number of proteins involved in a myriad of physiological and pathological processes, but the underlying mechanism of action via posttranslational modification has not been extensively explored. In this study, we investigated a novel modulatory mode of CHIP and its effect on CHIP enzymatic activity. ISG15, an ubiquitin-like modifier, is induced by type I interferon (IFN) stimulation and can be conjugated to target proteins (ISGylation). Here we demonstrated that CHIP may be a novel target of ISGylation in HEK293 cells stimulated with type I IFN. We also found that Lys143/144/145 and Lys287 residues in CHIP are important for and target residues of ISGylation. Moreover, ISGylation promotes the E3 ubiquitin ligase activity of CHIP, subsequently causing a decrease in levels of oncogenic c-Myc, one of its many ubiquitination targets, in A549 lung cancer cells and inhibiting A549 cell and tumor growth. In conclusion, the present study demonstrates that covalent ISG15 conjugation produces a novel CHIP regulatory mode that enhances the tumor-suppressive activity of CHIP, thereby contributing to the antitumor effect of type I IFN.
CD40L mediated alternative NFκB-signaling induces resistance to BCR-inhibitors in patients with mantle cell lymphoma
Authors: Rauert-Wunderlich Hilka , Rudelius Martina , Berberich Ingolf , Rosenwald Andreas 
Published: January 2018

AbstractDrug resistance is a significant obstacle in cancer treatment and therefore a frequent subject of research. Developed or primary resistance limits the treatment success of inhibitors of the B cell receptor (BCR) pathway in mantle cell lymphoma (MCL) patients. Recent research has highlighted the role of the nuclear factor-kappa B (NFκB) pathway in the context of resistance to BCR inhibitors in MCL. In this study, we analyzed the dependency of MCL cell lines on NFκB signaling and illustrated the ability of CD40L to activate the alternative NFκB pathway in MCL. This activation leads to independency of classical NFκB signaling and results in resistance to BCR inhibitors. Therefore, ligands (such as CD40L) and their activation of the alternative NFκB pathway have a major impact on the drug response in MCL. Furthermore, this study indicates a protective role for cells expressing specific ligands as microenvironmental niches for MCL cells and underlines the significance of therapeutically targeting alternative NFκB signaling in MCL.
Contaminant-Activated Visible Light Photocatalysis
Authors: Krishna Vijay , Bai Wei , Han Zhao , Yano Akihiro , Thakur Abhinav , Georgieva Angelina , Tolley Kristy , Navarro Joseph , Koopman Ben , Moudgil Brij 
Published: January 2018

AbstractPristine titanium dioxide (TiO2) absorbs ultraviolet light and reflects the entire visible spectrum. This optical response of TiO2 has found widespread application as white pigments in paper, paints, pharmaceuticals, foods and plastic industries; and as a UV absorber in cosmetics and photocatalysis. However, pristine TiO2 is considered to be inert under visible light for these applications. Here we show for the first time that a bacterial contaminant (Staphylococcus aureus—a MRSA surrogate) in contact with TiO2 activates its own photocatalytic degradation under visible light. The present study delineates the critical role of visible light absorption by contaminants and electronic interactions with anatase in photocatalytic degradation using two azo dyes (Mordant Orange and Procion Red) that are highly stable because of their aromaticity. An auxiliary light harvester, polyhydroxy fullerenes, was successfully used to accelerate photocatalytic degradation of contaminants. We designed a contaminant-activated, transparent, photocatalytic coating for common indoor surfaces and conducted a 12-month study that proved the efficacy of the coating in killing bacteria and holding bacterial concentrations generally below the benign threshold. Data collected in parallel with this study showed a substantial reduction in the incidence of infections.
Optimizing critical parameters for the directly measurement of particle flow with PF-SIBS
Authors: Yao Shunchun , Xu Jialong , Zhang Lifeng , Zhao Jingbo , Lu Zhimin 
Published: January 2018

AbstractA novel measurement technology named as particle flow-spark induced breakdown spectroscopy (PF-SIBS) was reported for real-time measurement of solid materials. Critical measurement parameters of PF-SIBS were optimized and a set of fly ashes with different carbon content were measured for evaluation of measurement performance. Four electrode materials, tungsten, copper, molybdenum and platinum, were compared in the aspects of signal stability, line interference and electrode durability. Less line interference and better signal stability were obtained with W and Cu electrode, while W electrode has better durability. Quartz sand with diameters from 48 μm to 180 μm were tested to investigate the influence of particle size. As the particle diameter increased, the intensity of Si 288.16 nm line decreased while that of ambient air constituents increased. To reduce the particle effect, the sum intensity from sample and ambient air were introduced to correct. The RSD of line intensity between the five diameters were reduced from 67.30% to 16.59% with Cu electrodes and from 63.21% to 13.64% with W electrodes. With the optimal measurement parameters and correction, fly ash samples with different carbon content were tested and the correlation coefficients R2 of multivariate calibration achieved 0.987.
Insights into earthquake hazard map performance from shaking history simulations
Authors: Vanneste Kris , Stein Seth , Camelbeeck Thierry , Vleminckx Bart 
Published: January 2018

AbstractWhy recent large earthquakes caused shaking stronger than shown on earthquake hazard maps for common return periods is under debate. Explanations include: (1) Current probabilistic seismic hazard analysis (PSHA) is deficient. (2) PSHA is fine but some map parameters are wrong. (3) Low-probability events consistent with a map sometimes occur. This issue has two parts. Verification involves how well maps implement PSHA (“have we built the map right?”). Validation asks how well maps forecast shaking (“have we built the right map?”). We explore how well a map can ideally perform by simulating an area’s shaking history and comparing “observed” shaking to that predicted by a map generated for the same parameters. The simulations yield shaking distributions whose mean is consistent with the map, but individual shaking histories show large scatter. Infrequent large earthquakes cause shaking much stronger than mapped, as observed. Hence, PSHA seems internally consistent and can be regarded as verified. Validation is harder because an earthquake history can yield shaking higher or lower than the hazard map without being inconsistent. As reality gives only one history, it is hard to assess whether misfit between a map and actual shaking reflects chance or a map biased by inappropriate parameters.
Biological corneal inlay for presbyopia derived from small incision lenticule extraction (SMILE)
Authors: Liu Yu-Chi , Teo Ericia Pei Wen , Ang Heng Pei , Seah Xin Yi , Lwin Nyein Chan , Yam Gary Hin Fai , Mehta Jodhbir S. 
Published: January 2018

AbstractCorneal inlays are a relatively new treatment option for presbyopia. Using biological inlays, derived from lenticules extracted from small incision lenticule extraction, may offer advantages over commercialized synthetic inlays in the aspect of biocompatibility. We conducted a non-human primate study to evaluate the safety, predictability, efficacy and tissue response after autogeneic, decellularized xenogeneic and xenogeneic lenticule implantation. The lenticule implantation effectively resulted in central corneal steepening (simulated keratometric values increased by 1.8–2.3 diopters), central hyper-prolate changes (asphericity Q values changed by −0.26 to −0.36), corneal anterior surface elevation (7.7–9.3 μm) and reasonable effective zone (1.5–1.8 times of the lenticule physical diameter), with no differences among the three groups. Slit lamp microscopy, transmission electron microscopy, confocal microscopy, histology and immunohistochemistry analyses confirmed the biocompatibility of the autogeneic and decellularized lenticules, whereas one eye in the xenogeneic group developed corneal stromal rejection during the study period. Our results showed that lenticule implantation has the potential for the management of presbyopia, and provide the basis for future clinical studies. The decellularization process may increase the potential utilization of lenticules without changing the efficacy.
Hematopoietic stem/progenitor cell senescence is associated with altered expression profiles of cellular memory-involved gene
Authors: Dong Yongpin , Lian Xiaolan , Xu Yanwu , Hu Haiyan , Chang Cen , Zhang Haiyin , Zhang Lina 
Published: January 2018

To evaluate the contributions of cellular memory mechanisms to hematopoietic stem/progenitor cell (HSPC) senescence. HSPCs (Lin−CD117+, hereafter referred to as HSPC) were separated from young (6-week-old) and aged (18-month-old) mice using Magnetic Activated Cell Sorting (MACS). Cell cycle distribution of HSPCs was determined using flow cytometry. The mixed colony forming unit (CFU-Mix) assay was used to study the HSPCs’ ability to proliferate. The mRNA expression levels of cellular memory-implicated PCG family (enhancer of zeste homolog 2 (Ezh2), B lymphoma mo-MLV insertion region 1 (Bmi-1), embryonic ectoderm development (Eed), melanoma nuclear protein 18 (Mel18), Mph1/polyhomeotic-like protein 1 (Rae-28)) and Trithorax group (TrxG) family (mixed lineage leukemia (Mll), thioredoxin (Trx)) were determined by quantitative real-time PCR. We obtained highly purified populations of mouse HSPCs (Lin−CD117+) (92.2 ± 4.5% CD117+). The percentage of HSPCs was significantly higher in older mice compared with younger control mice and the percentage of SA-β-galactosidase positive cells was significantly higher in HSPCs isolated from older mice (P&lt;0.05). The percentage of HSPCs in G0/G1 was significantly higher in older mice compared with younger control mice (52.0 compared with 47.1%), indicating increased cell cycle arrest in senescent HSPCs. The amount of CFU-Mix was significantly decreased in aged group (13.8 compared with 40.0), indicating a diminished ability to proliferate in senescent HSPCs. Ezh1, Bmi-1, Eed, Rae-28 gene mRNA expression was significantly lower in HSPCs from older mice compared to younger controls, while Mel18 mRNA expression was significantly higher in HSPCs from older mice (P&lt;0.05). The expression of genes associated with cellular memory is altered in senescent (Lin− CD117+) HSPCs, which may affect the potential plasticity of aged hematopoietic stem cells (HSCs) and thereby contribute to senescence-associated disease processes.
Hilfe zur Selbsthilfe
Authors: Rabenstein Lisa 
Published: January 2018

Zusammenfassung
Interventionskoffer, die Zwölfte Wie können Angst- und Panikpatienten praktikable Hilfen bekommen, um sich selbst zu helfen? Am
Pfalzklinikum wurde dazu eine psychoedukative Gruppe implementiert, die Betroffene anleitet, Ressourcen zu aktivieren, Wissen zu erwerben
und eigenverantwortlich mit ihrer Erkrankung umzugehen. Durch den dynamischen Austausch in der Gruppe können sie sich gegenseitig
unterstützen und Mut machen. Wir stellen Ihnen das Konzept und die dazugehörigen Werkzeuge vor.
Pionier der modernen Psychiatrie
Authors: Rau Fritz-Stefan 
Published: January 2018

Zusammenfassung
Porträt Heinz Häfner Kaum einer prägte die deutsche Psychiatrie der Nachkriegszeit wie der Psychiater Heinz Häfner. Was vielen nicht
bewusst ist: Er beeinflusste auch maßgeblich die psychiatrische Pflege. Wie er nach dem Zweiten Weltkrieg zu der Absicht kam, die
Psychiatrie zu reformieren, erzählt er im Gespräch mit der PPH.
Qualitätszeit schenken
Authors: Göhner Anne , Schnurr Klaus Bernhard , Saile Daniel , Bacherer Ralf , Hüll Prof. Dr. Michael 
Published: January 2018

Zusammenfassung
Ehrenamtlicher Besuchsdienst Wie lassen sich sogenannte „ehrenamtlich Begleitende“ erfolgreich in die Alterspsychiatrie integrieren?
Wir stellen Ihnen ein Schulungs- und Begleitkonzept für einen ehrenamtlichen Besuchsdienst vor, der an der Klinik für Alterspsychiatrie und
Psychotherapie Emmendingen entwickelt und erprobt wurde. Dabei ist die Intention der Besuche von Ehrenamtlichen klar von hilfspflegerischen
und therapeutischen Tätigkeiten abgegrenzt. Lesen Sie, wie es gelingt, „Qualitätszeit“ zu schenken, sodass alle Seiten davon profitieren –
sowohl die Patienten als auch die ehrenamtlich Begleitenden und die Pflegenden.
Latissimus Dorsi and Immediate Fat Transfer (LIFT) for Complete Autologous Breast Reconstruction
Authors: Economides James M. , Song David H. 
Published: January 2018

Background:
Despite the popularity of latissimus dorsi (LD) flap in breast reconstruction, a breast implant is often necessary to achieve sufficient volume. Prior reports describe fat grafting to the LD flap as a secondary procedure to correct contour deformities and improve volume. Our institution has instituted autologous breast reconstruction with an LD flap and immediate fat transfer (LIFT).

Methods:
A retrospective review of all patients undergoing the LIFT procedure was undertaken. Patient age, total volume of fat transfer, length of follow-up, need for adjuvant therapy, and complications were recorded. The procedure begins with harvest of the LD flap and fat. Prior to disorigination of the latissimus muscle, fat is injected into the flap. Flap harvest is then completed and inset to create a breast mound.

Results:
Eighteen patients underwent LIFT procedures over 3 years with an average follow-up of 8.7 months (range, 2–24). Four breasts (22.2%) had previously received adjuvant radiation therapy. The mean total fat grafting volume was 515.5 mL (range, 325–730) per breast. The average estimated fat graft take was 66.8% (range, 50–80%). Four patients (22.2%) experienced complications.

Conclusion:
Autologous augmentation of the LD flap with lipotransfer has been used to avoid placement of an implant. We improve the technique by performing lipotransfer during index reconstruction. Furthermore, we perform lipotransfer prior to disorigination of the LD muscle to minimize trauma to the flap and increase the efficiency of fat grafting. Our experience demonstrates that this technique is a viable autologous alternative to microsurgical breast reconstruction.
Long-term outcome following decompressive craniectomy: an inconvenient truth?
Authors: Honeybul Stephen , Ho Kwok M. , Gillett Grant R. 
Published: January 2018

Purpose of review
There is little doubt that decompressive craniectomy can reduce mortality following malignant middle cerebral infarction or severe traumatic brain injury. However, the concern has always been that the reduction in mortality comes at the cost of an increase in the number of survivors with severe neurological disability.

Recent findings
There has been a number of large multicentre randomized trials investigating surgical efficacy of the procedure. These trials have clearly demonstrated a survival benefit in those patients randomized to surgical decompression. However, it is only possible to demonstrate an improvement in outcome if the definition of favourable is changed such that it includes patients with either a modified Rankin score of 4 or upper severe disability. Without this recategorization, the results of these trials have confirmed the ‘Inconvenient truth’ that surgery reduces mortality at the expense of survival with severe disability.

Summary
Given these results, the time may have come for a nuanced examination of the value society places on an individual life, and the acceptability or otherwise of performing a procedure that converts death into survival with severe disability.
Neuro-ICU patient disposition: optimal venue for acute needs
Authors: Badenes Rafael , Robba Chiara , Taccone Fabio S. , Bilotta Federico 
Published: January 2018

Purpose of reviewThis article revises the recent evidence on ICU admission criteria for acute neurological patients [traumatic brain injury (TBI) patients, postoperative neurosurgical procedures and stroke].Recent findingsThe appropriate utilization of ICU beds is essential, but it is complex and a challenge to attain. To date there are no widely accepted international guidelines for managing these acute brain-injured patients (stroke, TBI, postneurosurgery) in the ICU. The criteria for ICU admission after neurological acute injury, high-dependency unit or a specialized neurosurgical ward vary from institution to institution depending on local structures and characteristics of the available resources. Better evidence to standardize the treatment and the degree of monitoring is needed during neurological acute injury. It is highly recommended to implement clinical vigilance in these patients regardless of their destination (ICU, stroke unit or ward).SummaryCurrently evidence do not allow to define standardized protocol to guide ICU admission for acute neurological patients (TBI patients, postoperative neurosurgical procedures and stroke).
Androgen abuse epidemiology
Authors: Sagoe Dominic , Pallesen Ståle 
Published: January 2018

Purpose of review
To systematically review current epidemiological literature on androgen abuse. Estimates from 32 empirical epidemiological articles were reviewed.

Recent findings
Generally, androgen abuse epidemiology and prevalence is higher in Europe, the Middle East, North America (the USA), Oceania (Australia and New Zealand), and South America (Brazil) and lower in Africa and Asia. In contrast to the general population, androgen abuse epidemiology and prevalence is higher among athletes, injection drug users, recreational sportspeople, and sexual and gender minorities.

Summary
From the 1970s, androgen abuse spread from athletes into the general population. Consistent with previous evidence, reviewed studies suggest that androgen abuse epidemiology and prevalence is higher in Western cultural contexts, the Middle East, and South America (Brazil) and lower in Africa and Asia. Evidence also corroborates indications that androgen abuse is less prevalent among women (vs. men), and in the general population in contrast to particular subpopulations consisting of athletes, injection drug users, recreational sportspeople, and sexual and gender minorities. Adolescents’ androgen abuse should be of special concern. Androgen abuse in some nonsports occupations (e.g. security workers) requires further exploration. Polypharmacy and the Internet proliferation of androgens and other PIEDs require surveillance for prevention and harm reduction.
Novel metabolic phenotypes in lecithin cholesterol acyltyransferase-deficient mice
Authors: Ng Dominic S. 
Published: January 2018

Purpose of review
Lecithin cholesterol acyltyransferase (LCAT) deficiency is a rare monogenic disorder causing lipoprotein dysregulation and multiple organ dysfunctions, including renal impairment. LCAT knockout mice have been shown informative in elucidating mechanisms of many major clinical morbid phenotypes. Extended characterization of the LDL receptor/LCAT double knockout (Ldlr/Lcat-DKO or DKO) mice had led to the discovery of a number of novel protective metabolic phenotypes, including resistance to obesity, nonalcoholic steatohepatitis (NASH) and insulin resistance. We seek to integrate the findings to explore novel pathogenic pathways.

Recent findings
The chow fed DKO mice were found more insulin sensitive than their Ldlr-KO controls. Joint analyses of the three strains (DKO, Ldlr-KO and wild-type) revealed differential metabolic responses to a high cholesterol diet (HCD) vs. high-fat diet (HFD). DKO mice are protected from HFD-induced obesity, hepatic endoplasmic reticulum (ER) stress, insulin resistance, ER cholesterol and NASH markers (steatosis and inflammasomes). Joint analysis revealed the HFD-induced NASH is dependent on de-novo hepatic cholesterol biosynthesis. DKO mice are protected from HCD-induced hepatic ER stress, ER cholesterol, but not NASH, the latter likely due to cholesterol crystal accumulation. DKO mice were found to develop ectopic brown adipose tissue (BAT) in skeletal muscle. Ectopic BAT derived in part from myoblast in utero and from adult satellite cells. Primed expression of PRDM16 and UCP in quiescent satellite cell caused by LCAT deficiency synergizes with cell cholesterol depletion to induce satellite cell-to-BAT transdifferentiation.

Summary
Metabolic phenotyping of selective LCAT null mice led to the discovery of novel metabolically protective pathways.