bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of NOP58 in prostate cancer progression through SUMOylation regulation and drug response
Authors: Guo Wei , Zong Shi , Liu Tao , Chao Yi , Wang Kaichen 
Published: October 2024

BackgroundProstate cancer is one of the leading causes of cancer-related deaths in men. Its molecular pathogenesis is closely linked to various genetic and epigenetic alterations, including posttranslational modifications like SUMOylation. Identifying biomarkers that predict outcomes and specific therapeutic targets depends on a comprehensive understanding of these processes. With growing interest in SUMOylation as a mechanism affecting prostate cancer-related genes, this study aimed to investigate the central role of SUMOylation in prostate cancer prognostics, focusing on the significance of NOP58.MethodsWe conducted a comprehensive bioinformatics analysis, integrating differential expression analysis, survival analysis, gene set enrichment analysis (GSEA), and single-cell transcriptomic analyses using data from The Cancer Genome Atlas (TCGA). Key genes were identified through intersections of Venn diagrams, Boralta algorithm signatures, and machine learning models. These signaling mechanisms were validated through experimental studies, including immunohistochemical staining and gene ontology analyses.ResultsThe dual-gene molecular subtype analysis with SUMO1, SUMO2, and XPO1 genes revealed significant differences in survival outcomes across molecular subtypes, further emphasizing the potential impact of NOP58 on SUMOylation, a key post-translational modification, in prostate cancer. NOP58 overexpression was strongly associated with shorter overall survival (OS), progression-free interval (PFI), and disease-specific death in prostate cancer patients. Immunohistochemical analysis confirmed that NOP58 was significantly overexpressed in prostate cancer tissues compared to normal tissues. ROC curve analysis demonstrated that NOP58 could distinguish prostate cancer from control samples with high diagnostic accuracy. Gene Ontology analysis, along with GSVA and GSEA, suggested that NOP58 may be involved in cell cycle regulation and DNA repair pathways. Moreover, NOP58 knockdown led to increased BCL2 expression and decreased Ki67 levels, promoting apoptosis and inhibiting cell proliferation. Colony formation assays further showed that NOP58 knockdown inhibited, while its overexpression promoted, colony formation, highlighting the critical role of NOP58 in prostate cancer cell growth and survival. Additionally, NOP58 was linked to drug responses, including Methotrexate, Rapamycin, Sorafenib, and Vorinostat.ConclusionNOP58 is a key regulator of prostate cancer progression through its mediation of the SUMOylation pathway. Its expression level serves as a reliable prognostic biomarker and an actionable therapeutic target, advancing precision medicine for prostate cancer. Targeting NOP58 may enhance therapeutic efficacy and improve outcomes in oncology.
Voice biomarkers in middle and later adulthood as predictors of cognitive changes
Authors: Mahon Elizabeth , Lachman Margie E. 
Published: October 2024

BackgroundProsody voice measures, especially jitter and shimmer, have been associated with cognitive impairment and hold potential as early indicators of risk for cognitive decline. Prior research suggests that voice measures assessed concurrently with longitudinal cognitive outcomes are associated with 10-year cognitive declines in middle-age and older adults from Midlife in the U.S. (MIDUS) study.ResultsUsing a subsample from the same study, we expanded previous research to examine voice measures that were (1) collected 8 years before cognitive outcomes, (2) derived from narrative speech in logical memory tests instead of word list recall tests, and (3) independent of the cognitive outcomes. Multilevel analyses controlled for covariates of age, sex, education, neurological conditions, depressive symptoms, and chronic conditions. The results indicated that higher jitter and lower shimmer predicted greater 10-year declines in episodic memory and working memory.ConclusionThese findings extend previous research by highlighting prosody voice measures assessed 8 years earlier as predictors of subsequent cognitive declines over a decade.
Older people in the world of esport: a qualitative study
Authors: Esnard Catherine , Haza Marion , Grangeiro Rebeca 
Published: October 2024

IntroductionThe inclusion of older players in the gaming world is growing rapidly, leading to the emergence of specific categories for them at major esport events. The aim of this study is to analyze the psychological issues faced by older people when engaging with the competitive context of esport.Materials and methodsThe study was conducted in 2021 within the French association Silver Geek, which offers digital workshops using Nintendo Wii consoles to older people living in retirement homes and organizes annual amateur competitions between teams of older adults. This qualitative study involved 16 participants (eight women, eight men) aged 63 to 97 years. Semi-structured interviews were used to explore the motivations, emotional well-being, and social impact of esport on older people.ResultsThe results show that older people who participate in esport are motivated by the discovery and mastery of new game skills, as well as the pleasure of performance through social comparison. All experience a psychological well-being that translates into intense positive emotions. Moreover, the esport context, whether during the pre-competition workshops or the competitions themselves, proved to be a strong vector for positive social relationships, especially with the young e-sports coaches. However, this activity had little impact on existing family relationships.ConclusionThis study highlights the potential of esport as a recreational activity to promote mental health and social integration in older adults. The challenge for future research is to identify the levers that will enable this activity to become a true medium for intergenerational communication.
Falls and fall-related injuries: prevalence, characteristics, and treatment among participants of the Geelong Osteoporosis Study
Authors: Yosef Tewodros , Pasco Julie A. , Tembo Monica C. , Williams Lana J. , Holloway-Kew Kara L. 
Published: October 2024

BackgroundFalls are a significant public health challenge, especially among older adults. In Australia, falls and related injuries incur an annual cost of $2.3 billion. However, there is a scarcity of prevalence data on falls among population-based groups. This study aimed to report the characteristics, circumstances, and treatment for falls and fall-related injuries in a population-based sample of Australian men and women.MethodsParticipants from the Geelong Osteoporosis Study provided cross-sectional data: baseline for men (2001–2006) and 10-year follow-up for women (2004–2008). Falls over the previous 12 months were self-reported by 2,631 participants aged 20–97 years (1,533 men and 1,098 women). The study described the timing, location, cause, and treatment of falls. Fall prevalence was standardized to the 2006 Australian population. Data collection included self-reported prior fractures, medication, disease conditions, anthropometry, and biochemical tests. Binary logistic regression identified factors linked to fall-related injuries.ResultsFall rates by age group: 20–29 (24.4%), 30–39 (21.5%), 40–49 (18.7%), 50–59 (24.9%), 60–69 (25.0%), 70–79 (34.6%), 80+ (40.5%). The age-standardized prevalence of falls was 25.0% (95% CI: 23.4–26.7%). In adults ≥65 years, the age-standardized prevalence of falls was 32.4% (95% CI: 29.3–35.5%). Fallers were typically older, less mobile, had higher BMI and cholesterol levels, and were more likely to have musculoskeletal conditions, cancer, and polypharmacy. Men had a higher fall risk, but fewer fall-related fractures compared to women. Most falls occurred outdoors (62.1%), were due to extrinsic cause (58.5%), and were on the same level (78.5%). Factors associated with fall-related injuries included being female, elevated falls and falls outside the home. Nearly half (45.7%) of those injured sought medical attention, primarily from general practitioners (25.7%) and emergency departments (12.6%).ConclusionFalls are common, occurring in approximately one quarter of adults in this study, with a greater prevalence among those ≥65 years. Women experienced fewer multiple falls than men. Many participants sought medical attention, primarily from general practitioners. This research identifies fall characteristics and circumstances, informing targeted prevention strategies to reduce occurrences and alleviate burdens on healthcare systems and individuals.
Impact of pleural reconstruction on postoperative outcomes in rib tumor resection: a decade-long retrospective study
Authors: Xie Hao , Li Bowen , Sun Yixin , Ma Lin , Zhang Qiang 
Published: October 2024

ObjectiveThis study aims to evaluate the effects of pleural reconstruction during rib compartment tumor resection surgery on postoperative outcomes, including drainage volume, drainage duration, hospital stay, complications, and pain control.MethodsA retrospective analysis was conducted on 142 patients who underwent rib compartment tumor resection surgery at Beijing Jishuitan Hospital from January 2013 to October 2023. The patients were divided into two groups: those who received pleural reconstruction and those who did not. Data were collected from hospital medical records and outpatient care records, focusing on postoperative drainage volume, total drainage time, length of hospital stay, complications, and pain scores. Continuous variables were compared using t-tests or nonparametric tests, while categorical variables were analyzed using chi-square tests or Fisher's exact tests.ResultsThe analysis showed no significant differences between the two groups in terms of postoperative complications and pain thresholds. However, patients who underwent pleural reconstruction had significantly lower postoperative drainage volume (937.74 ± 855.97 vs. 1,595.26 ± 1,054.50 ml, p &lt; 0.05), shorter drainage duration (5.5 ± 2.39 vs. 8.43 ± 2.87 days, p &lt; 0.05), and reduced length of hospital stay (7.32 ± 3.30 vs. 10.99 ± 6.83 days, p &lt; 0.05).ConclusionPleural reconstruction during rib compartment tumor resection surgery reduces postoperative drainage volume, drainage duration, and hospital stay without increasing complications or short-term pain. Further large-scale studies are recommended to validate these findings.
Insecticide Resistance and Target-Site Mutations kdr, N1575Y, and Ace-1 in Anopheles gambiae s.l. Populations in a Low-Malaria-Transmission Zone in the Sudanian Region of Senegal
Authors: Gueye Assiyatou , Ngom El Hadji Malick , Ndoye Baye Bado , Dione Mamadou Lamine , Diouf Babacar , Ndiaye El Hadji , Sy Faty Amadou , Guèye Marième , Niang Makhtar , Diallo Diawo , Diallo Mawlouth , Dia Ibrahima 
Published: October 2024

Background/Objectives: Significant progress in malaria control has been achieved through long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS), raising hopes for malaria elimination. However, emerging insecticide resistance threatens these gains. This study assessed the susceptibility of Anopheles gambiae s.l. populations to public health insecticides, examined the frequencies of kdr, Ace-1, and N1575Y mutations, and explored their associations with phenotypic resistance in Dielmo and Ndiop, Senegal. Methods: Anopheles larvae collected between September and December 2022 were reared to adulthood. Adult mosquitoes were exposed to discriminating concentrations of various insecticides following WHO guidelines. Knockdown times (KDT50 and KDT95) for pyrethroids were calculated using the Probit model. RT-qPCR detected target-site mutations (kdr: L1014F and L1014S, Ace-1, N1575Y) and assessed correlations with phenotypic resistance. Species-specific PCR identified species within the An. gambiae complex. Results/Conclusions: The populations of Dielmo and Ndiop showed susceptibility to pirimiphos-methyl and bendiocarb, with no Ace-1 mutation detected. Resistance to DDT and pyrethroids was observed. The knockdown times indicated that alphacypermethrin and lambdacyhalothrin were more effective than permethrin and deltamethrin. The L1014F allele was widespread, while L1014S was absent in Ndiop and low in Dielmo. The N1575Y mutation occurred only in populations with L1014F. The L1014S mutation was significantly associated with resistance to lambdacyhalothrin in both villages and to deltamethrin in Ndiop.
Expression Profiles of Fatty Acid Transporters and the Role of n-3 and n-6 Polyunsaturated Fatty Acids in the Porcine Endometrium
Authors: Blitek Agnieszka , Szymanska Magdalena 
Published: October 2024

Fatty acids (FAs) are important for cell membrane composition, eicosanoid synthesis, and metabolic processes. Membrane proteins that facilitate FA transport into cells include FA translocase (also known as CD36) and FA transporter proteins (encoded by SLC27A genes). The present study aimed to examine expression profiles of FA transporters in the endometrium of cyclic and early pregnant gilts on days 3 to 20 after estrus and the possible regulation by conceptus signals and polyunsaturated FAs (PUFAs). The effect of PUFAs on prostaglandin (PG) synthesis and transcript abundance of genes related to FA action and metabolism, angiogenesis, and immune response was also determined. Day after estrus and reproductive status of animals affected FA transporter expression, with greater levels of CD36, SLC27A1, and SLC27A4 observed in pregnant than in cyclic gilts. Conceptus-conditioned medium and/or estradiol-17β stimulated SLC27A1 and CD36 expression. Among PUFAs, linoleic acid decreased SLC27A1 and SLC27A6 mRNA expression, while arachidonic, docosahexaenoic, and eicosapentaenoic acids increased SLC27A4 transcript abundance. Moreover, arachidonic acid stimulated ACOX1, CPT1A, and IL1B expression and increased PGE2 and PGI2 secretion. In turn, α-linolenic acid up-regulated VEGFA, FGF2, FABP4, and PPARG mRNA expression. These results indicate the presence of an active transport of FAs in the porcine endometrium and the role of PUFAs as modulators of the uterine activity during conceptus implantation.
SỬ DỤNG CANXI CHLORUA (CaCl2) TRONG CANH TÁC ĐỂ HẠN CHẾ TÌNH TRẠNG NỨT TRÁI, CHẠY CHỈ TRÊN TRÁI VÚ SỮA TÍM (Chrysophyllum cainito L.) KHI THU HOẠCH TẠI HUYỆN KẾ SÁCH, TỈNH SÓC TRĂNG
Authors: Nguyễn Thị Thuở  , Nguyễn Thị Bích Hằng  , Vũ Bá Quan  , Trần Văn Toàn  , Vũ Quan  , Lê Trung Tâm  
Published: October 2024

USING CALCIUM CHLORIDE (CaCl 2) SPRAY ON FRUITS TO LIMIT FRUIT CRACKING AND THREADING ON PURPLE STAR APPLE FRUITS (Chrysophyllum cainito L.) WHEN HARVESTING IN KE SACH DISTRICT, SOC TRANG PROVINCE, VIETNAM
Tóm tắt: Vùng trồng và cung ứng vú sữa xuất khẩu chính của tỉnh Sóc Trăng hiện nay là huyện Kế Sách. Tuy nhiên, gần đến ngày thu hoạch thì trái hay bị nứt hoặc chạy chỉ, làm giảm năng suất, giảm chất lượng sản phẩm. Mục tiêu của bài báo là xác định liều lượng Canxi Chlorua (CaCl2) và thời điểm phun để hạn chế tình trạng nứt trái, chạy chỉ trên trái vú sữa tím trên vườn vú sữa tím 07 năm tuổi tại xã Xuân Hòa (huyện Kế Sách, tỉnh Sóc Trăng). Thí nghiệm được bố trí theo thể thức khối hoàn toàn ngẫu nhiên, hai nhân tố, (1) là mức hàm lượng CaCl2 (có 3 mức là 0,5%, 2,0% và 4,0%), đối chứng không phun CaCl2 và (2) là thời điểm phun CaCl2 (phun 2 lần: 90 và 120 ngày, 120 và 150 ngày sau khi đậu trái). Kết quả cho thấy, phun CaCl2 nồng độ 2,0% hai lần vào lúc 90 và 120 ngày sau đậu trái cho thấy trái có tỉ lệ nứt trái thấp nhất (17,2%), trái không bị chạy chỉ; đồng thời, trọng lượng, đường kính và độ ngọt (Brix) của trái tương đương đối chứng, phù hợp với thị trường tiêu thụ trong và ngoài nước.
Abstract: Ke Sach district is a prominent area of star apple fruit growing and supplying for export in Soc Trang province currently. However, close to the harvest date, the fruit cracking phenomenon or threading on the fruit’s skin appears, reducing productivity and product quality. The experiment was conducted to determine the dose of calcium chloride (CaCl2) and the time of spraying to reduce fruit cracking and threading on purple star apple fruit at 7-year-old purple star apple fruit orchard in Xuan Hoa Communes (Ke Sach District, Soc Trang Province). The experiment was done using a completely randomized block design, with two factors (1) dose of CaCl2 (0.5%, 2.0%, and 4.0%), non-sprayed CaCl2 as control, and (2) the time of spraying CaCl2 (spray twice: 90 and 120 days, 120 and 150 days after fruit set). The results showed that spraying CaCl2 twice at a concentration of 2.0% at 90 and 120 days after the fruit set showed that the rate of fruit cracking was lowest (17,2%) and no threading on the fruit’s skin; at the same time, the weight, diameter, and sweetness (Brix) of the fruit are equivalent to the control, suitable for domestic and export markets.
ПРЕДПРИНИМАТЕЛЬСКАЯ КУЛЬТУРА КАК ФАКТОР РАЗВИТИЯ БИЗНЕС-СИСТЕМ
Authors: Елина О.А. , Дедусенко Е.А. 
Published: October 2024

На сегодняшний день системы в бизнесе – это не модный термин, а тренд и ключевая стратегия в развитии современных экономик и предпринимательства. В условиях ускорения технологического прогресса, бизнес-системы представляют собой сложные сети взаимодействий между компаниями, стартапами, инвесторами, государством и обществом. Они способствуют созданию инновационного и взаимосвязанного предпринимательского окружения, которое стимулирует экономический рост и развитие. В этой статье мы рассмотрим, как бизнес-системы влияют на экономику и предпринимательство, а также какие факторы способствуют их успеху и развитию. Авторы сосредоточили внимание на исследовании предпринимательской культуры в России. Исследование показывает, что в современном мире, где экономика и бизнес постоянно эволюционируют, предпринимательская культура играет ключевую роль в развитии и укреплении бизнес-систем. Она не только влияет на способы ведения бизнеса, но и определяет, как компании адаптируются к изменениям, инновациям и конкуренции. В этой статье мы рассмотрим, как предпринимательская культура формирует основу для сильных и устойчивых бизнес-систем, способствуя росту и инновациям на всех уровнях экономики. В заключение внесены предложения в национальную программу формирования и развития предпринимательской культуры, которая будет способствовать повышению предпринимательской активности бизнес-систем и экономическому росту.
Today, ecosystems in business are not a fashionable term, but a trend and a key strategy in the development of modern economies and entrepreneurship. In the context of accelerating technological progress, business ecosystems are complex networks of interactions between companies, startups, investors, the state and society. They contribute to the creation of an innovative and interconnected business environment that stimulates economic growth and development. In this article we will look at how business ecosystems affect the economy and entrepreneurship, as well as what factors contribute to their success and development. The authors focus on the study of entrepreneurial culture in Russia. The study shows that in today's world where the economy and business are constantly evolving, entrepreneurial culture plays a key role in the development and strengthening of business ecosystems. It not only influences the way businesses do business, but also determines how companies adapt to change, innovation, and competition. In this article we will look at how entrepreneurial culture forms the basis for strong and sustainable business ecosystems, fostering growth and innovation at all levels of the economy. The article concludes with suggestions for a national program to build and develop an entrepreneurial culture that will enhance business system entrepreneurship and economic growth.
ЗАРУБЕЖНЫЕ МЕТОДЫ ОЦЕНКИ РЕЗУЛЬТАТИВНОСТИ ЗЕЛЕНЫХ ИННОВАЦИЙ ПРОМЫШЛЕННЫХ ПРЕДПРИЯТИЙ
Authors: Сивкова А.И. , Подшивалова М.В. 
Published: October 2024

Промышленные отрасли отличаются различной сложностью производственно-технического процесса, ввиду этого и оценка результативности зеленых инноваций промышленных предприятий должна учитывать данную дифференциацию. В данной работе проанализированы зарубежные методы оценки результативности зеленых инноваций в промышленности на макро-уровне. Были рассмотрены два подхода к оценке результативности эко-инноваций: метод патентной активности и метод интегральной оценки. В рамках метода интегральной оценки были изучены индексы эко-инноваций Европейской комиссии и Форума «Азия – Европа». Были выявлены особенности расчета, преимущества и недостатки данных методов. Основная гипотеза исследования заключается в том, что зарубежные методы оценки результативности зеленых инноваций на макро-уровне в недостаточной степени учитывают показатели, связанные с промышленными отраслями. В статье использованы такие методы, как теоретический анализ и синтез, сравнение, классифицирование. В результате подтверждено, что имеющиеся зарубежные методы имеют недостатки для оценки результативности зеленых инноваций с точки зрения промышленности. В заключении делается вывод о необходимости разработки такого метода оценки эко-инноваций, который будет учитывать дифференциацию промышленных отраслей и рассматривать качественные и количественные показатели.
Industrial sectors are distinguished by varying complexity of the production and technical process; therefore, assessing the performance of green innovations of industrial enterprises should take this differentiation into account. This paper analyzes foreign methods for assessing the performance of green innovations in industry at the macro level. Two approaches to assessing the performance of eco-innovations were considered: the patent analysis and the index method. Within the framework of the index method, the eco-innovation indices of the European Commission and the Asia-Europe Forum were studied. The calculation features, advantages and disadvantages of these methods were identified. The main hypothesis of the study is that foreign methods for assessing the performance of green innovations at the macro level do not sufficiently take into account indicators related to industrial sectors. The article uses methods such as theoretical analysis and synthesis, comparison, classification. As a result, it was confirmed that existing foreign methods have shortcomings for assessing the performance of green innovations from an industrial point of view. It is concluded that it is necessary to develop a method for assessing eco-innovations that will take into account the differentiation of industrial sectors and consider qualitative and quantitative indicators.