bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased CD14+HLA-DR-/low myeloid-derived suppressor cells can be regarded as a biomarker on disease severity and response to therapy in acute coronary syndrome
Authors: Tan Yinsheng , Ren Mingde , Hou Jie , Hou Tao , Lin Xianhe 
Published: October 2024

Purpose
This study aimed to investigate the dynamic changes in monocytic myeloid-derived suppressor cells (M-MDSCs) and their implications in the pathogenesis of acute coronary syndrome (ACS), shedding light on potential therapeutic targets.

Experimental Design
Peripheral blood samples were collected from 68 ACS patients, 35 stable angina pectoris (SAP) patients, and 30 healthy controls (HC). Multi-parameter flow cytometry was employed for analysis of M-MDSCs, explored with disease characteristics and progression.

Results
ACS patients exhibited an increased frequency of circulating M-MDSCs compared to SAP patients and HC. M-MDSCs levels demonstrated associations with ACS type, coronary artery lesions, multi-vessel disease, and cardiac dysfunction severity. Higher M-MDSCs levels were found in obese patients. Notably, therapy led to a significant decrease in M-MDSCs frequency. Furthermore, ACS patients exhibited elevated levels of interleukin (IL)-6, IL-10, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α) in the cytokine profile associated with M-MDSCs. Increased expression of arginase-1(Arg-1) was observed in ACS patients, with positive correlations between M-MDSCs levels and IL-6, GM-CSF, and Arg-1 expression. The diagnostic performance of triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and M-MDSCs levels varied in predicting the severity of coronary artery stenosis, with TG showing higher specificity, HDL-C displaying higher sensitivity, and M-MDSCs levels demonstrating balanced sensitivity and specificity.

Conclusions
Assessment of M-MDSCs frequency holds promise as a predictive marker for disease progression and therapy response of coronary artery stenosis. The elevated presence of M-MDSCs suggests their potential role in modulating ACS-related inflammation.
Contribution of socio-demographic factors in prevalence of soil-transmitted helminth infections among newly arrived laborers in Jeddah, Saudi Arabia
Authors: Wakid Majed H. , Al-Refai Mohammad F. 
Published: October 2024

Background
Soil-transmitted diseases are caused by intestinal worms transmitted via various routes into the human body resulting in various clinical manifestations. This study aimed to investigate the socio-demographic factors contributing mainly to the prevalence of soil-transmitted helminths (STHs) among newly arrived laborers in Jeddah, Saudi Arabia.

Methods
A total of 188 stool specimens were collected and examined macroscopically and microscopically using different techniques. In addition, real-time PCR was used as a molecular tool to detect several STHs. The descriptive analysis was used to evaluate demographic data and categorical variables in association with STHs infection.

Results
Of all participants, the mean age was 26.08 ± 2.67 years, including 103 females and 85 males. A total of 86 (45.70%) were illiterate, followed by 60 (31.90%) of participants belonging to elementary level. STHs and other intestinal parasites were detected among 35 newly arrived laborers (18.61%). Nineteen cases (10.11%) were infected with STHs, including 15 cases with one STHs (four A. lumbricoides, four N. americanus, four T. trichiura, two S. stercoralis, one A. duodenale) and four cases with two STHs (two T. trichiura and N. americanus; one S. stercoralis and A. duodenale; one T. trichiura and S. stercoralis). High rates of STHs infection were detected among drivers (11.70%) followed by housemaids (11.20%), but with no significant association to occupation. Higher rates of STHs infection were detected among those used to walk on soil barefoot in their home countries before arriving in Jeddah.

Conclusion
This study found that none of the newly arrived expatriate laborers in Jeddah was aware of STHs. About 10% of them had infection with one or two STHs, mainly among newly arrived laborers from Asian countries. There is a need for awareness programs and regular screening for STHs and other intestinal parasites among newly arrived expatriate laborers.
Cross-cultural adaptation and psychometric properties of the Arabic version of the Central Sensitization Inventory in people with chronic musculoskeletal pain
Authors: Tamboosi Sarah E. , Alzahrani Hosam , Alshehri Fahad H. , Alzhrani Msaad , S. Alshehri Yasir 
Published: October 2024

Background
The Central Sensitization Inventory (CSI) is a patient-reported screening instrument that can be used to identify and assess central sensitization (CS)/Central Sensitization Syndrome (CSS)-related symptoms.

Objective
The aim was to translate the CSI into Arabic (CSI-Ar) and to subsequently validate its psychometric properties.

Design
Cross-sectional.

Methods
The CSI was translated and cross-culturally adapted into Arabic, and validated following international standardized guidelines. This study included patients with chronic musculoskeletal pain (n = 264) and healthy control participants (n = 56). Patients completed the CSI-Ar, Pain Catastrophizing Scale (PCS), Depression, Anxiety, and Stress scale (DASS-21), Tampa Scale of Kinesiophobia (TSK), and 5-level EuroQol-5D (EQ-5D). Patients completed the CSI-Ar twice to assess test–retest reliability. To evaluate discriminative validity, healthy controls participants completed the CSI-Ar. Statistical analyses were conducted to test the internal consistency, reliability, and structural, construct and discriminant validity of CSI-Ar.

Results
The CSI-Ar showed acceptable internal consistency (Cronbach’s alpha = 0.919) and excellent test–retest reliability (intraclass correlation coefficient = 0.874). The CSI-Ar scale had significant correlations (P < 0.001) with all PCS subscales and total score (Spearman’s rho = 0.459–0.563, P < 0.001), all DASS-21 subscales and total score (Spearman’s rho = 0.599–0.685, P < 0.001), the TSK (Spearman’s rho = 0.395, P < 0.001), and the EQ-5D (Spearman’s rho = −0.396, P < 0.001). The Mann-Whitney U-test showed a statistically significant difference between the patient group and the healthy control group (P < 0.001), with the healthy controls displaying a lower average CSI-Ar score (12.27 ± 11.50) when compared to the patient group (27.97 ± 16.08). Factor analysis indicated that the CSI-Ar is a unidimensional tool.

Conclusion
The CSI-Ar is a reliable and valid screening tool that can be used to assess CS/CSS-related symptoms in Arabic-speaking people with chronic musculoskeletal pain.
Big Data in cognitive sciences, didactics, education management, and evidence-based education policy
Authors: Agatova Olga 
Published: October 2024

The book reveals the methodology of big data analysis in the field of education - a new direction in science and practice. The book is addressed to education researchers who are interested in the application of data science in education and who are developing a data-driven approach in education and human development research. The book is also addressed to heads of educational organizations and heads of regional education systems, teachers and methodologists of various educational institutions.
The book characterizes methods for analyzing educational data and data on education at the levels of educational analytics, institutional analytics, regional, regional (territorial) analytics.
The content of the book reveals the development of information anthropology and cybersemiotics in expanding the arsenal of methods for analyzing human-generated educational data. Characterizes the development of the cognitive-didactic concept of digital educational environments in the interdisciplinary field of social cognitive science and data science.
The book presents the results of research and development on changing the structure of competencies of pedagogical and managerial activities in the field of education, predicts changes in the system of qualifications in the context of the digital transformation of education.
Standardization of Hormone-Based Seed Coating Formulation for Coriander Dosage and Formulation Optimization
Authors: Palani-Vasantha Suriyaprakash , Shanmugam Kavitha , Ranganathan Umarani , Sethuraman Lakshmi , Arumugam Thangahemavathy , Duraisamy ThirusenduraSelvi , Ramasamy Vigneshwari , Venugopal Vijayageetha , Kali Parameswari , Chinnasamy Pradipa , Jayakumar HarishBalaji , Thirumaniselvam Kavichakravarthi 
Published: October 2024

Aims: This study aimed to standardize the dosage of hormone-based seed coating formulations to enhance germination and seedling growth in coriander seeds.
Study Design: Completely Randomized Design.
Place and Duration of Study: Department of seed science and technology, Tamil Nadu Agricultural University, Coimbatore, India. During the year of 2023-2024.
Methodology: Coriander seeds were coated with different concentrations of seed coating polymer and germination study was conducted in laboratory using rolled towel method with four replications. Data were analyzed by ANOVA with significance at p ≤ 0.05.
Results: The hormone-based seed coating formulation has recorded significantly higher germination% (69%), root length (16.75 cm), shoot length (7.9 cm), dry matter production (0.058 g/10 seedlings), Vigour Index I (1706) and II (3.9) at 10 g polymer/kg of seed and 290 mL of water.
Conclusion: Seed Coating with 10 seed coating formulation dissolved in 290 mL of water enhanced the seed germination and seedling growth.
Coke vs. Pepsi: Brand Compatibility, Relationship Power, and Life Satisfaction
Authors: Brick Danielle J , Fitzsimons Gràinne M , Chartrand Tanya L , Fitzsimons Gavan J 
Published: June 2017

Abstract
Individuals often evaluate, purchase, and consume brands in the presence of others, including close others. Yet relatively little is known about the role brand preferences play in relationships. In the present research, the authors explore how the novel concept of brand compatibility, defined as the extent to which individuals have similar brand preferences (e.g., both partners prefer the same brand of soda), influences life satisfaction. The authors propose that when brand compatibility is high, life satisfaction will also be high. Conversely, because low brand compatibility may be a source of conflict for the relationship, the authors propose that it will be associated with reduced life satisfaction. Importantly, the authors predict that the effects of brand compatibility on conflict and life satisfaction will depend upon relationship power. Across multiple studies and methodologies, including experimental designs (studies 2, 3, 5) and dyadic data from real-life couples (studies 1, 4, 6), the authors test and find support for their hypotheses. By exploring how a potentially unique form of compatibility influences life satisfaction, including identifying a key moderator and an underlying mechanism, the current research contributes to the literatures on branding, close relationships, consumer well-being, and relationship power.
High-Grade Glioma is not a Feature of Neurofibromatosis Type 2 in the Unirradiated Patient
Authors: King Andrew T , Rutherford Scott A , Hammerbeck-Ward Charlotte , Lloyd Simon K , Freeman Simon M , Pathmanaban Omar N , Rodriguez-Valero Monica , Thomas Owen M , Laitt Roger D , Stivaros Stavros , Kellett Mark , Evans D Gareth 
Published: June 2017

Abstract

BACKGROUND
The Manchester criteria for neurofibromatosis type 2 (NF2) include a range of tumors, and gliomas were incorporated in the original description. The gliomas are now widely accepted to be predominantly spinal cord ependymomas.

OBJECTIVE
To determine whether these gliomas include any cases of malignant glioma (WHO grade III and IV) through a database review.

METHODS
The prospective database consists of 1253 patients with NF2. 1009 are known to be alive at last follow-up.

RESULTS
There was a single case of glioblastoma multiforme (GBM; World Health Organization grade IV) in the series and no WHO grade III gliomas. The GBM was in a patient who had previously undergone stereotactic radiosurgery for a vestibular schwannoma.

CONCLUSION
High-grade gliomas are not a feature of NF2 in the unirradiated patient and should be excluded from the diagnostic criteria.
Resident Macrophages are Locally Programmed for Silent Clearance of Apoptotic Cells
Authors: Roberts Allison W. , Lee Bettina L. , Deguine Jacques , John Shinu , Shlomchik Mark J. , Barton Gregory M. 
Published: June 2017

SUMMARYAlthough apoptotic cells (ACs) contain nucleic acids that can be recognized by Toll-like receptors (TLRs), engulfment of ACs does not initiate inflammation in healthy organisms. To better understand this phenomenon, we identified and characterized macrophage populations that continually engulf ACs in several distinct tissues. These macrophages share characteristics compatible with immunologically silent clearance of ACs, including high expression of AC recognition receptors, low expression of TLR9, and reduced TLR responsiveness to nucleic acids. When removed from tissues these macrophages lose many of these characteristics and generate inflammatory responses to AC-derived nucleic acids, suggesting that cues from the tissue microenvironment are required to program macrophages for silent AC clearance. We show that KLF2 and KLF4 control expression of many genes within this AC clearance program. Coordinated expression of AC receptors with genes that limit responses to nucleic acids may represent a central feature of tissue macrophages that ensures maintenance of homeostasis.
Multi-omic mitoprotease profiling defines a role for Oct1p in coenzyme Q production
Authors: Veling Mike T. , Reidenbach Andrew G. , Freiberger Elyse C. , Kwiecien Nicholas W. , Hutchins Paul D. , Drahnak Michael J. , Jochem Adam , Ulbrich Arne , Rush Matthew J.P. , Coon Joshua J. , Pagliarini David J. 
Published: June 2017

SUMMARYMitoproteases are becoming recognized as key regulators of diverse mitochondrial functions, although their direct substrates are often difficult to discern. Through multi-omic profiling of diverseSaccharomyces cerevisiaemitoprotease deletion strains, we predicted numerous associations between mitoproteases and distinct mitochondrial processes. These include a strong association between the mitochondrial matrix octapeptidase Oct1p and coenzyme Q (CoQ) biosynthesis—a pathway essential for mitochondrial respiration. Through Edman sequencing, andin vitroandin vivobiochemistry, we demonstrated that Oct1p directly processes the N-terminus of the CoQ-related methyltransferase, Coq5p, which markedly improves its stability. A single mutation to the Oct1p recognition motif in Coq5p disrupted its processingin vivo, leading to CoQ deficiency and respiratory incompetence. This work defines the Oct1p processing of Coq5p as an essential post-translational event for proper CoQ production. Our custom data visualization tool enables efficient exploration of mitoprotease profiles that can serve as the basis for future mechanistic investigations.
Xolik: finding cross-linked peptides with maximum paired scores in linear time
Authors: Dai Jiaan , Jiang Wei , Yu Fengchao , Yu Weichuan 
Published: June 2017

AbstractMotivationCross-linking technique coupled with mass spectrometry (MS) is widely used in the analysis of protein structures and protein-protein interactions. In order to identify cross-linked peptides from MS data, we need to consider all pairwise combinations of peptides, which is computationally prohibitive when the sequence database is large. To alleviate this problem, some heuristic screening strategies are used to reduce the number of peptide pairs during the identification. However, heuristic screening criteria may ignore true findings.ResultsWe directly tackle the combination challenge without using any screening strategies. With the additive scoring function and the data structure of double-ended queue, the proposed algorithm reduces the quadratic time complexity of exhaustive searching down to the linear time complexity. We implement the algorithm in a tool named Xolik, and the running time of Xolik is validated using databases with different number of proteins. Experiments using synthetic and empirical datasets show that Xolik outperforms existing tools in terms of running time and statistical power.AvailabilitySource code and binaries of Xolik are freely available athttp://bioinformatics.ust.hk/Xolik.html.Contacteeyu@ust.hkSupplementary informationSupplementary data are available atBioinformaticsonline.
Input-specific NMDAR-dependent potentiation of dendritic GABAergic inhibition
Authors: Chiu Chiayu Q. , Martenson James S. , Yamazaki Maya , Natsume Rie , Sakimura Kenji , Tomita Susumu , Tavalin Steven J. , Higley Michael J. 
Published: June 2017

SummaryPreservation of a balance between synaptic excitation and inhibition is critical for normal brain function. A number of homeostatic cellular mechanisms have been suggested to play a role in maintaining this balance, including long-term plasticity of GABAergic inhibitory synapses. Many previous studies have demonstrated a coupling of postsynaptic spiking with modification of perisomatic inhibition. Here, we demonstrate that activation of NMDA-type glutamate receptors leads to input-specific long-term potentiation of dendritic inhibition mediated by somatostatin-expressing interneurons. This form of plasticity is expressed postsynaptically and requires both CaMKIIα and the β2-subunit of the GABA-A receptor. Importantly, this process may function to preserve dendritic inhibition, asin vivoloss of NMDAR signaling results in a selective weakening of dendritic inhibition. Overall, our results reveal a new mechanism for linking excitatory and inhibitory input in neuronal dendrites and provide novel insight into the homeostatic regulation of synaptic transmission in cortical circuits.
High-quality genome assemblies uncover caste-specific long non-coding RNAs in ants
Authors: Shields Emily J. , Bonasio Roberto 
Published: June 2017

ABSTRACTAnts are an emerging model system for neuroepigenetics, as embryos with virtually identical genomes develop into different adult castes that display strikingly different physiology, morphology, and behavior. Although a number of ant genomes have been sequenced to date, their draft quality is an obstacle to sophisticated analyses of epigenetic gene regulation. Using long reads generated with Pacific Biosystem single molecule real time sequencing, we have reassembledde novohigh-quality genomes for two ant species:Camponotus floridanusandHarpegnathos saltator. The long reads allowed us to span large repetitive regions and join sequences previously found in separate scaffolds, leading to comprehensive and accurate protein-coding annotations that facilitated the identification of aGp-9-likegene as differentially expressed inHarpegnathoscastes. The new assemblies also enabled us to annotate long non-coding RNAs for the first time in ants, revealing several that were specifically expressed duringHarpegnathosdevelopment and in the brains of different castes. These upgraded genomes, along with the new coding and non-coding annotations, will aid future efforts to identify epigenetic mechanisms of phenotypic and behavioral plasticity in ants.
An improved experimental model of cystic hydatid disease in liver resembling natural infection route with stable growing dynamics and immune reaction
Authors: Zhang Ruiqing , Chen Xinhua , Wen Hao 
Published: June 2017

AbstractCystic echinococcosis is an endemic parasitic infection in Xinjiang, China and is causing serious economic burdens and public health concerns. An experimental murine modelin vivofor hepatic cystic echinococcosis was established in C57B/6 mice by injection with human protoscolices via the portal vein of three different concentrations. Mice were followed up 10 months by ultrasound, gross anatomy, pathological and immunological examinations. The protoscolice migration in portal vein, hydatid cyst growth, host immune reaction and hepatic histopathology were examed periodicly. The infection rate of the mice in the high, medium, and low concentration groups were 90%, 100%, and 63.6%, respectively. The protoscolices migrate in the portal vein with blood flow, settle in the liver and develop into orthotopic hepatic hydatid cysts, resembling the natural infection route and course. This study established an improved experimental model of low biohazard risk but stable growing dynamics and immune reaction. It is especially useful for new anti-parasite medication trials agains hydatid disease.Summary statementAn experimental murine model of cystic echinococcosis was set up. This orthotopic model resembles primary infection route and natural infectious course with low biohazard risk and high efficiency.
A cerebellar mechanism for learning prior distributions of time intervals
Authors: Narain Devika , Jazayeri Mehrdad 
Published: June 2017

AbstractKnowledge about the statistical regularities of the world is essential for cognitive and sensorimotor function. In the domain of timing, prior statistics are crucial for optimal prediction, adaptation and planning. Where and how the nervous system encodes temporal statistics, however, is not known. Deriving from physiological and anatomical evidence for cerebellar learning, we develop a computational model that demonstrates how the cerebellum could learn prior distributions of time intervals and support Bayesian temporal estimation. The model shows that salient features observed in human Bayesian time interval estimates can be readily captured by learning in the cerebellar cortex and circuit level computations in the cerebellar deep nuclei.
Characterizing divergence through three adjacent Australian avian transition zones
Authors: Peñalba Joshua V. , Mason Ian J. , Schodde Richard , Moritz Craig , Joseph Leo 
Published: June 2017

AbstractAimThe diversification of the Australian avifauna has been greatly influenced by prominent historical and modern barriers to dispersal. The aims of this study were to characterize the patterns of divergence in population pairs of meliphagoid birds across adjacent transition zones and characterize how well morphometric divergence, habitat association and taxonomic or species ranking can predict genetic divergence.LocationNorthern Queensland, Australia.MethodsGenetic divergence between parental populations on either side of the three biogeographical barriers corresponding to three clusters of hybrid zones was characterized in 27 species complexes of meliphagoid birds using one mitochondrial, 23 autosomal and 12 Z chromosome loci collected from a sequence capture system. Within each species, we characterized morphometric divergence using wing, bill and tail measurements from museum samples. Lastly, we evaluated the predictive power of these morphometric measurements on genetic divergence.ResultsPopulation pairs on either side of a transition zone depict a wide range of genomic and morphometric divergence. For some systems, species exhibiting morphometric divergence show little to no genomic divergence, while, conversely, other species exhibiting little to no morphometric divergence may show clear genomic divergence. Species rank is shown to be the strongest predictor for genetic divergence, habitat is the next strongest predictor and morphometric divergence is the weakest predictor.Main conclusionsThe variation in divergence levels of population pairs affirms that transition zones are ideal natural experiments to study the speciation process. In particular, transition zones allow understanding of how genomic divergence accumulates during speciation. Additionally, standing species rank classifications mostly prove to be robust after genetic characterization. Lastly, the discordance between morphometric and genetic divergence suggests other non‐morphometric phenotypic traits used to designate species rank, such as song or plumage, may play a more important role in predicting genetic divergence.