bluuebunny/crossref_metadata_2025_split huggingface dataset:
A proteomics method using immunoaffinity fluorogenic derivatization–liquid chromatography/tandem mass spectrometry (FD‐LC‐MS/MS) to identify a set of interacting proteins
Authors: Nakata Katsunori , Saitoh Ryoichi , Ishigai Masaki , Imai Kazuhiro 
Published: August 2017

AbstractBiological functions in organisms are usually controlled by a set of interacting proteins, and identifying the proteins that interact is useful for understanding the mechanism of the functions. Immunoprecipitation is a method that utilizes the affinity of an antibody to isolate and identify the proteins that have interacted in a biological sample. In this study, the FD‐LC–MS/MS method, which involves fluorogenic derivatization followed by separation and quantification by HPLC and finally identification of proteins by HPLC–tandem mass spectrometry, was used to identify proteins in immunoprecipitated samples, using heat shock protein 90 (HSP90) as a model of an interacting protein in HepaRG cells. As a result, HSC70 protein, which was known to form a complex with HSP90, was isolated, together with three different types of HSP90‐beta. The results demonstrated that the proposed immunoaffinity–FD‐LC–MS/MS method could be useful for simultaneously detecting and identifying the proteins that interact with a certain protein.
Cervical sensorimotor control in idiopathic cervical dystonia: A cross‐sectional study
Authors: De Pauw Joke , Mercelis Rudy , Hallemans Ann , Michiels Sarah , Truijen Steven , Cras Patrick , De Hertogh Willem 
Published: August 2017

AbstractObjectivesPatients with idiopathic adult‐onset cervical dystonia (CD) experience an abnormal head posture and involuntary muscle contractions. Although the exact areas affected in the central nervous system remain uncertain, impaired functions in systems stabilizing the head and neck are apparent such as the somatosensory and sensorimotor integration systems. The aim of the study is to investigate cervical sensorimotor control dysfunction in patients with CD.Material and MethodsCervical sensorimotor control was assessed by a head repositioning task in 24 patients with CD and 70 asymptomatic controls. Blindfolded participants were asked to reposition their head to a previously memorized neutral head position (NHP) following an active movement (flexion, extension, left, and right rotation). The repositioning error (joint position error, JPE) was registered via 3D motion analysis with an eight‐camera infrared system (VICON® T10). Disease‐specific characteristics of all patients were obtained via the Tsui scale, Cervical Dystonia Impact Profile (CDIP‐58), and Toronto Western Spasmodic Rating Scale.ResultsPatients with CD showed larger JPE than controls (mean difference of 1.5°, p < .006), and systematically ‘overshoot’, i.e. surpassed the NHP, whereas control subjects ‘undershoot’, i.e. fall behind the NHP. The JPE did not correlate with disease‐specific characteristics.ConclusionsCervical sensorimotor control is impaired in patients with CD. As cervical sensorimotor control can be trained, this might be a potential treatment option for therapy, adjuvant to botulinum toxin injections.
INST OX‐05‐024: first line gemcitabine, oxaliplatin, and erlotinib for primary hepatocellular carcinoma and bile duct cancers: a multicenter Phase II trial
Authors: Patt Yehuda Z. , Murad Waheed , Fekrazad Mohammed H. , Baron Ari D. , Bansal Pranshu , Boumber Yanis , Steinberg Kim , Lee Sang‐Joon , Bedrick Ed , Du Ruofei , Lee Fa Chyi 
Published: August 2017

AbstractHepatocellular Carcinoma (HCC) incidence is increasing in the USA. Gemcitabine (G) and oxaliplatin (O) are active in HCC and biliary duct cancer (BDC). Erlotinib (E) is an EGFR tyrosine kinase inhibitor (TKI) with known activity against both. We sought to evaluate the efficacy of the combination G+O+E. Patients with either of the two diagnosis were treated in a phase II trial. Simons 2 stage design was used. A disease‐control rate (DCR), complete response (CR) + partial response (PR)+ stable disease (SD) at 24 weeks of ≤20% and >40% (P0 and P1 of 0.2 and 0.4, respectively) were set as undesirable (null) and desirable results. 26 HCC and 7 BDC patients were accrued. In HCC, 1 PR, 10 SD, and 9 PDs were seen. DCR in HCC was 42%. Among seven (7) patients with BDC, one patient was not evaluable; one achieved a long lasting PR, and five patients had SD and DCR was 86%. Median overall survival (OS) times and progression‐free survivals (PFS) were 196 and 149 days in HCC and 238 days and not reached in BDC. PFS at 26 weeks in HCC was 41% and at 21 weeks in BDC was 60%. Grade 3 toxicities in >5% of patients were fatigue (12.9%), neutropenia (9.6%), thrombocytopenia (9.6%), and diarrhea (6.4%). G+O+E exceeded both preset P0a and P1 of the primary objective with a PFS of 41% at 26 weeks for HCC and preliminary BDC data may warrant further investigations.
Structural Modification of Stilbenoids from Acanthopanax leucorrhizus and Their Cytotoxic Activity
Authors: Hu Hao‐Bin , Liang Hai‐Peng , Li Hai‐Ming , Yuan Ru‐Nan , Sun Jiao , Wu Yun , Zhang La‐La , Han Ming‐Hu 
Published: August 2017

A new cis‐stilbenoid, 1,9‐dihydroxy‐10‐methoxy‐6H‐dibenzo[b,f]oxocin‐6‐one (2) was isolated from the AcOEt extract of the stem barks of Acanthopanax leucorrhizus, along with three known stilbenoids, 9‐hydroxy‐10‐methoxy‐6H‐dibenzo[b,f]oxocin‐6‐one (1), 5‐O‐methyl‐(E)‐resveratrol 3‐O‐β‐d‐glucopyranoside (3), and (E)‐resveratrol 3‐O‐β‐d‐xylopyranoside (4). Two derivatives (2a and 2b) were synthesized by the structural modification of compound 2, which exhibited certain cytotoxic activities against HT‐29 and HeLa cell lines in vitro. All compounds were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (HL‐60, HT‐29, and HeLa) by the standard MTT assay in vitro. The results showed that derivatives 2a and 2b exhibited strong activities than compounds 2 against HT‐29 and HeLa cell lines.
Organo‐Photoredox Catalyzed Oxidative Dehydrogenation of N‐Heterocycles
Authors: Sahoo Manoj K. , Jaiswal Garima , Rana Jagannath , Balaraman Ekambaram 
Published: August 2017

AbstractWe report here for the first time the catalytic oxidative dehydrogenation of N‐heterocycles by a visible‐light organo‐photoredox catalyst with low catalyst loading (0.1–1 mol %). The reaction proceeds efficiently under base‐ and additive‐free conditions with ambient air at room temperature. The utility of this benign approach is demonstrated by the synthesis of various pharmaceutically relevant N‐heteroarenes such as quinoline, quinoxaline, quinazoline, acridine, and indole.
Continuous Production of Biorenewable, Polymer‐Grade Lactone Monomers through Sn‐β‐Catalyzed Baeyer–Villiger Oxidation with H2O2
Authors: Yakabi Keiko , Mathieux Thibault , Milne Kirstie , López‐Vidal Eva M. , Buchard Antoine , Hammond Ceri 
Published: August 2017

AbstractThe Baeyer–Villiger oxidation is a key transformation for sustainable chemical synthesis, especially when H2O2 and solid materials are employed as oxidant and catalyst, respectively. 4‐substituted cycloketones, which are readily available from renewables, present excellent platforms for Baeyer–Villiger upgrading. Such substrates exhibit substantially higher levels of activity and produce lactones at higher levels of lactone selectivity at all values of substrate conversion, relative to non‐substituted cyclohexanone. For 4‐isopropyl cyclohexanone, which is readily available from β‐pinene, continuous upgrading was evaluated in a plug‐flow reactor. Excellent selectivity (85 % at 65 % conversion), stability, and productivity were observed over 56 h, with over 1000 turnovers (mol product per mol Sn) being achieved with no loss of activity. A maximum space–time yield that was almost twice that for non‐substituted cyclohexanone was also obtained for this substrate [1173 vs. 607 g(product) kg(catalyst)−1 cm−3 h−1]. The lactone produced is also shown to be of suitable quality for ring opening polymerization. In addition to demonstrating the viability of the Sn‐β/H2O2 system to produce renewable lactone monomers suitable for polymer applications, the substituted alkyl cyclohexanones studied also help to elucidate steric, electronic, and thermodynamic elements of this transformation in greater detail than previously achieved.
Immunological tolerance to LCMV antigens differently affects control of acute and chronic virus infection in mice
Authors: Woopen Christina , Straub Tobias , Schweier Oliver , Aichele Ulrike , Düker Katharina , Boehm Thomas , Pircher Hanspeter 
Published: August 2017

AbstractCytotoxic T lymphocytes (CTLs) play a key role in the control of lymphocytic choriomeningitis virus (LCMV) infection. In C57BL/6 mice (H‐2b), the CTL response is mainly directed against epitopes from the LCMV glycoprotein (GP) and the nucleoprotein (NP) which represent the two major viral proteins. The role of GP‐ versus NP‐derived epitopes for viral clearance was examined using transgenic (tg) mice ubiquitously expressing LCMV GP and NP, respectively. These mice lack GP‐ or NP‐specific CTLs and show decreased levels of GP‐ or NP‐specific antibodies as a result of tolerance induction. During acute LCMV infection, CTLs specific for GP‐ and NP‐derived epitopes are generated with similar frequencies. Nonetheless, we found that lack of GP‐ but not of NP‐specific CTLs abolished control of acute LCMV infection. In contrast, after high‐dose or chronic LCMV infection, virus elimination was delayed to a similar extent in GP‐ and NP‐tg mice. Thus, immunological tolerance to LCMV antigens differently affects virus clearance in acute and chronic infection settings. In addition, our data reveal that immunodominance of H‐2b‐restricted LCMV‐specific CTL epitopes and their antiviral activity do not strictly correlate.
Flow-covariate prediction of stream pesticide concentrations
Authors: Mosquin Paul L. , Aldworth Jeremy , Chen Wenlin 
Published: August 2017

Abstract

Potential peak functions (e.g., maximum rolling averages over a given duration) of annual pesticide concentrations in the aquatic environment are important exposure parameters (or target quantities) for ecological risk assessments. These target quantities require accurate concentration estimates on nonsampled days in a monitoring program. We examined stream flow as a covariate via universal kriging to improve predictions of maximum m-day (m = 1, 7, 14, 30, 60) rolling averages and the 95th percentiles of atrazine concentration in streams where data were collected every 7 or 14 d. The universal kriging predictions were evaluated against the target quantities calculated directly from the daily (or near daily) measured atrazine concentration at 32 sites (89 site-yr) as part of the Atrazine Ecological Monitoring Program in the US corn belt region (2008–2013) and 4 sites (62 site-yr) in Ohio by the National Center for Water Quality Research (1993–2008). Because stream flow data are strongly skewed to the right, 3 transformations of the flow covariate were considered: log transformation, short-term flow anomaly, and normalized Box-Cox transformation. The normalized Box-Cox transformation resulted in predictions of the target quantities that were comparable to those obtained from log-linear interpolation (i.e., linear interpolation on the log scale) for 7-d sampling. However, the predictions appeared to be negatively affected by variability in regression coefficient estimates across different sample realizations of the concentration time series. Therefore, revised models incorporating seasonal covariates and partially or fully constrained regression parameters were investigated, and they were found to provide much improved predictions in comparison with those from log-linear interpolation for all rolling average measures. Environ Toxicol Chem 2018;37:260–273. © 2017 SETAC
Numerical and experimental study of free convection through a horizontal open‐ended axisymmetric cavity
Authors: Ait Haj Said Abdessadek , Chehouani Hassan , Er‐Raki Salah 
Published: August 2017

AbstractThis paper summarizes a numerical and experimental investigation of free convective heat transfer in an open‐ended cavity between two horizontal parallel circular plates. The upper plate is maintained at an ambient temperature and the lower one is heated. Air is used as the heat transfer medium. The numerical model equations are solved using a control volume‐based finite differences method, and the experimental study was performed using holographic interferometry. Streamlines and isotherm patterns are presented and discussed for different aspect ratios (A) and Rayleigh numbers (Ra). Heat transfer at the surface of the lower plate is thoroughly inspected in the ranges and . Useful correlations of Nusselt numbers in terms of and A are given with their validity ranges. Also, an investigation of both numerical and experimental results is performed. It shows similar temperature field aspect with some differences in the radial boundary layer thickness and a small deviation in the heat transfer.
The inhibitory effect of 5,7‐DMF on pancreatic sphere‐forming cell function mediated by FoxM1 gene expression
Authors: Zeng Deyu , Ma Jian , Li Rongrong , Yang Jianfeng , Yin Xianli 
Published: August 2017

AbstractPancreatic cancer is one of the major human malignant tumors severely endangering human health and life with high mortality due to the concealment of early symptoms and lack of effective therapies during advanced stages. The identification of pancreatic cancer stem cell functions has been as important strategy for understanding of pancreatic cancer biology and novel drug and therapy development. In the present study, we successfully isolated the pancreatic sphere‐forming cells from pancreatic cancer cell line PANC‐1 by sphere‐forming method and we found that the sphere‐forming ability and the cell migration rate of pancreatic sphere‐forming cells were significantly inhibited by 5,7‐DMF treatment, which was supported by the corresponding changes of several EMT biomarkers after being treated with 5,7‐DMF. Moreover, we revealed here that the inhibition of pancreatic sphere‐forming cells was mediated by the expression of FoxM1 gene, and also the expression of SOX2 gene was regulated by FoxM1 in pancreatic sphere‐forming cells and involved in the inhibitory role of 5,7‐DMF. These results provided important basis for the application of 5,7‐DMF as a novel drug candidate for the pancreatic cancer treatment.
Lysozyme oxidation by singlet molecular oxygen: Peptide characterization using [18O]‐labeling oxygen and nLC‐MS/MS
Authors: Marques Emerson Finco , Medeiros Marisa H.G. , Di Mascio Paolo 
Published: August 2017

AbstractSinglet molecular oxygen (1O2) is generated in biological systems and reacts with different biomolecules. Proteins are a major target for 1O2, and His, Tyr, Met, Cys, and Trp are oxidized at physiological pH. In the present study, the modification of lysozyme protein by 1O2 was investigated using mass spectrometry approaches. The experimental findings showed methionine, histidine, and tryptophan oxidation. The experiments were achieved using [18O]‐labeled 1O2 released from thermolabile endoperoxides in association with nano‐scale liquid chromatography coupled to electrospray ionization mass spectrometry. The structural characterization by nLC‐MS/MS of the amino acids in the tryptic peptides of the proteins showed addition of [18O]‐labeling atoms in different amino acids.
Restoration of thalamo‐cortical connectivity after brain injury: recovery of consciousness, complex behavior, or passage of time?
Authors: Crone Julia S. , Bio Branden J. , Vespa Paul M. , Lutkenhoff Evan S. , Monti Martin M. 
Published: August 2017

AbstractIn 2000, a landmark case report described the concurrent restoration of consciousness and thalamo‐frontal connectivity after severe brain injury (Laureys et al., ). Being a single case however, this study could not disambiguate whether the result was specific to the restoration of consciousness per se as opposed to the return of complex cognitive function in general or simply the temporal evolution of post‐injury pathophysiological events. To test whether the restoration of thalamo‐cortical connectivity is specific to consciousness, 20 moderate‐to‐severe brain injury patients (from a recruited sample of 42) underwent resting‐state functional magnetic resonance imaging within a week after injury and again six months later. As described in the single case report, we find thalamo‐frontal connectivity to be increased at the chronic, compared with the acute, time‐point. The increased connectivity was independent of whether patients had already recovered consciousness prior to the first assessment or whether they recovered consciousness in‐between the two. Conversely, we did find an association between restoration of thalamo‐frontal connectivity and the return of complex cognitive function. While we did replicate the findings of Laureys et al. (), our data suggests that the restoration of thalamo‐frontal connectivity is not as tightly linked to the reemergence of consciousness per se. However, the degree to which the return of connectivity is linked to the return of complex cognitive function, or to the evolution of other time‐dependent post‐injury mechanisms, remains to be understood.
Mechanism study of multimode ultrasound pretreatment on the enzymolysis of wheat gluten
Authors: Zhang Yanyan , Li Jing , Li Suyun , Ma Haile , Zhang Hua 
Published: August 2017

AbstractBACKGROUNDUltrasound pretreatment could improve the angiotensin‐I converting enzyme (ACE) inhibitory activity of hydrolysates of wheat gluten (WG). The working mode of ultrasound has an important effect on the enzymatic hydrolysis of protein.RESULTSThe results showed that the optimum working mode of ultrasound was alternate dual‐frequency mode (20/35 kHz), substrate concentration was 30 g L−1, initial temperature of the suspension was 30 °C, ultrasound pretreatment time was 10 min and power density was 150 W L−1. Under optimised conditions, ACE inhibitory activity of WG hydrolysates reached to its maximum value in advance. The surface hydrophobicity (H0) of WG and the content of small peptides at the beginning of the enzymolysis were improved by the ultrasound pretreatment. The structure of WG was destroyed by the ultrasound pretreatment. The enzymatic residue of ultrasound pretreated WG were damaged greater than control.CONCLUSIONIt was concluded that alternate dual‐frequency ultrasound pretreatment improved the ACE inhibitory activity. Ultrasonic pretreatment may loosen the tissue of WG aggregate, and help the enzyme alcalase to attack the interior of WG aggregate easily, which resulted in the release of low molecular weight peptides from WG aggregate. © 2017 Society of Chemical Industry
Large orbital defect reconstruction in the setting of globe‐sparing maxillectomy: The titanium hammock and layered fibula technique
Authors: Trosman Samuel J. , Haffey Timothy M. , Couto Rafael A. , Fritz Michael A. 
Published: August 2017

AbstractIntroductionThe purpose of our study was to describe a novel technique for reconstruction of orbital defects after maxillectomy using a non‐anatomic titanium mesh suspension of orbital contents for both support and volume correction. This construct is then articulated with a layered fibula osteocutaneous free flap that restores orbital rim, zygoma, and maxillary alveolus. We herein present our application of this technique, including refinements over time and long‐term outcomes.MethodsA retrospective review was performed on 12 patients who underwent reconstruction of Brown class III orbitopalatomaxillary defects with extensive orbital involvement (at minimum complete orbital floor and rim absent) with titanium mesh sling and a layered fibula free flap.ResultsPrimary reconstruction was accomplished in all 12 patients. The mean postoperative length of stay was 8 days (6–14 days). There were no free flap failures or perioperative re‐explorations. Patients were routinely extubated on postoperative day #1 and began oral intake by postoperative day #3. At a mean follow‐up length of 48 months, unrestricted eye function was accomplished in all patients. Midfacial symmetry was accomplished in 10 of 12 patients; 2 patients had moderate asymmetry due to extirpation of facial musculature and/or soft tissue. Minor revisions were necessary for lower lid ectropion and exposure of the titanium plate. Two patients required adipofascial free flap coverage of exposed intraoral bone after radiation therapy.ConclusionsThis technique provides excellent restoration of eye position and function and also allows for implant‐based prosthetic rehabilitation. It has become our procedure of choice for orbitomaxillary reconstruction.
Planar piecewise linear random motions with jumps
Authors: Garra Roberto , Orsingher Enzo , Ratanov Nikita 
Published: August 2017

In this paper, we study persistent piecewise linear multidimensional random motions. Their velocities, switching at Poisson times, are uniformly distributed on a sphere. The changes of direction are accompanied with subsequent jumps of random length and of uniformly distributed orientation.In this paper, we obtain some useful properties and formulae of distributions of these processes. In particular, we get these distributions in the cases of jumps with Gaussian and exponential distributions of jump magnitudes.
High‐mobility group box 1 released by autophagic cancer‐associated fibroblasts maintains the stemness of luminal breast cancer cells
Authors: Zhao Xi‐Long , Lin Yong , Jiang Jun , Tang Zhuo , Yang Shuai , Lu Lu , Liang Yan , Liu Xue , Tan Jiao , Hu Xu‐Gang , Niu Qin , Fu Wen‐Juan , Yan Ze‐Xuan , Guo De‐Yu , Ping Yi‐Fang , Wang Ji Ming , Zhang Xia , Kung Hsiang‐Fu , Bian Xiu‐Wu , Yao Xiao‐Hong 
Published: August 2017

AbstractCancer stem cells/cancer‐initiating cells (CICs) and their microenvironmental niche play a vital role in malignant tumour recurrence and metastasis. Cancer‐associated fibroblasts (CAFs) are major components of the niche of breast cancer‐initiating cells (BCICs), and their interactions may profoundly affect breast cancer progression. Autophagy has been considered to be a critical process for CIC maintenance, but whether it is involved in the cross‐talk between CAFs and CICs to affect tumourigenesis and pathological significance has not been determined. In this study, we found that the presence of CAFs containing high levels of microtubule‐associated protein 1 light chain 3 (LC3II), a marker of autophagosomes, was associated with more aggressive luminal human breast cancer. CAFs in human luminal breast cancer tissues with high autophagy activity enriched BCICs with increased tumourigenicity. Mechanistically, autophagic CAFs released high‐mobility group box 1 (HMGB1), which activated its receptor, Toll‐like receptor (TLR) 4, expressed by luminal breast cancer cells, to enhance their stemness and tumourigenicity. Furthermore, immunohistochemistry of 180 luminal breast cancers revealed that high LC3II/TLR4 levels predicted an increased relapse rate and a poorer prognosis. Our findings demonstrate that autophagic CAFs play a critical role in promoting the progression of luminal breast cancer through an HMGB1–TLR4 axis, and that both autophagy in CAFs and TLR4 on breast cancer cells constitute potential therapeutic targets. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.