bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fact learning in complex arithmetic—the role of the angular gyrus revisited
Authors: Bloechle Johannes , Huber Stefan , Bahnmueller Julia , Rennig Johannes , Willmes Klaus , Cavdaroglu Seda , Moeller Korbinian , Klein Elise 
Published: May 2016

AbstractIn recent theoretical considerations as well as in neuroimaging findings the left angular gyrus (AG) has been associated with the retrieval of arithmetic facts. This interpretation was corroborated by higher AG activity when processing trained as compared with untrained multiplication problems. However, so far neural correlates of processing trained versus untrained problems were only compared after training. We employed an established learning paradigm (i.e., extensive training of multiplication problems) but measured brain activation before and afte training to evaluate neural correlates of arithmetic fact acquisition more specifically. When comparing activation patterns for trained and untrained problems of the post‐training session, higher AG activation for trained problems was replicated. However, when activation for trained problems was compared to activation for the same problems in the pre‐training session, no signal change in the AG was observed. Instead, our results point toward a central role of hippocampal, para‐hippocampal, and retrosplenial structures in arithmetic fact retrieval. We suggest that the AG might not be associated with the actual retrieval of arithmetic facts, and outline an attentional account of the role of the AG in arithmetic fact retrieval that is compatible with recent attention to memory hypotheses. Hum Brain Mapp 37:3061–3079, 2016. © 2016 Wiley Periodicals, Inc.
Management of orbital invasion in sinonasal malignancies
Authors: Lisan Quentin , Kolb Frédéric , Temam Stéphane , Tao Yungan , Janot François , Moya‐Plana Antoine 
Published: May 2016

AbstractBackgroundIndications for preservation of orbital contents in case of malignant sinonasal tumors invading the orbit remain controversial.MethodsA retrospective consecutive review was conducted of patients in a tertiary care center over a 15‐year period.ResultsNinety‐three patients were diagnosed with a malignant tumor invading the orbit. Eighty‐three were treated with curative intent of which 58 underwent surgery. Mean follow‐up was 45 months. Orbital preservation was feasible in 66% of cases, whereas orbital clearance was performed in cases of locally advanced disease (invasion of extraocular muscles, ocular globe, or orbital apex). Local control rate was 70% for patients treated with orbital clearance and 74% for those with orbital preservation, with no statistical difference. Five‐year survival and 5‐year relapse‐free survival were similar in both groups.ConclusionUsing our strategy, the eye can be spared in more patients than what is currently proposed, without oncologic or survival disadvantages. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1708–1716, 2016
Complications after surgery for benign parotid gland neoplasms: A prospective cohort study
Authors: Ruohoalho Johanna , Mäkitie Antti A. , Aro Katri , Atula Timo , Haapaniemi Aaro , Keski–Säntti Harri , Takala Annika , Bäck Leif J. 
Published: May 2016

AbstractBackgroundProspective studies on procedure‐specific incidences of complications after benign parotid surgery are lacking. Predictive factors for postoperative facial dysfunction remain controversial.MethodsWe conducted a prospective study on 132 patients undergoing parotid surgery for benign parotid neoplasms. We analyzed complication rates and assessed risk factors of postoperative transient facial palsy.ResultsFacial palsy rate was 40.2% on the first postoperative day, 28.3% at 2 weeks, 3.9% at 6 months, and 1.6% at 12 months. Immediate postoperative palsy rates in subgroups of partial superficial parotidectomy, superficial parotidectomy, extended parotidectomy, and ECD were 41.5%, 43.8%, 53.8%, and 6.3%, respectively. Age, duration of surgery, and use of ultrasound knife were identified as risk factors for transient facial palsy.ConclusionDepending on the operation type, up to half of the patients experience facial palsy after benign parotid surgery. Higher age and longer duration of operation increase the risk. The role of operative instrumentation requires further studies. © 2016 Wiley Periodicals, Inc. Head Neck 39: 170–176, 2017
Ubiquitin-Specific Protease 4 Antagonizes Osteoblast Differentiation Through Dishevelled
Authors: Zhou Fangfang , Li Fang , Fang Pengfei , Dai Tong , Yang Bing , van Dam Hans , Jia Junling , Zheng Min , Zhang Long 
Published: May 2016

ABSTRACT

The canonical Wnt/β-catenin signaling pathway plays a pivotal role and is essentially required for the osteoblast differentiation and bone formation. In this study, we found ubiquitin-specific peptidase 4 (USP4) to strongly inhibit the Wnt/β-catenin signaling by removing Lysine-63 linked poly-ubiquitin chain from Dishevelled (Dvl). Ectopic expression of USP4 promoted β-catenin poly-ubiquitination and thus inhibited Wnt-induced accumulation of cytosolic β-catenin and counteracted Wnt-induced transcriptional activity. Moreover, USP4 knockdown or USP4 knockout led to an increase in the active β-catenin levels and in activation of Wnt/β-catenin-induced transcription. Functional studies in C2C12 myoblasts and KS483 osteoprogenitor cells showed that ectopic expression of USP4 resulted in impaired activation of endogenous Wnt3a-induced genes and decreased osteoblast differentiation and mineralization, whereas USP4 depletion showed the opposite effect. These results identify USP4 as a novel regulator of Dvl in Wnt/β-catenin signal and show its involvement in Wnt3a-induced osteoblast differentiation. © 2016 American Society for Bone and Mineral Research.
Angiogenesis and Inflammation Crosstalk in Diabetic Retinopathy
Authors: Capitão Margarida , Soares Raquel 
Published: May 2016

ABSTRACTDiabetic retinopathy (DR) is one of the most prevalent microvascular complications of diabetes and one of the most frequent causes of blindness in active age. Etiopathogenesis behind this important complication is related to several biochemical, hemodynamic and endocrine mechanisms with a preponderant initial role assumed by polyol pathways, increment of growth factors, accumulation of advanced glycation end products (AGE), activation of protein kinase C (PKC), activation of the renin‐angiotensin‐aldosterone system (RAAS), and leukostasis. Chronic and sustained hyperglycemia works as a trigger to the early alterations that culminate in vascular dysfunction. Hypoxia also plays an essential role in disease progression with promotion of neovascularization and vascular dystrophies with vitreous hemorrhages induction. Thus, the accumulation of fluids and protein exudates in ocular cavities leads to an opacity augmentation of the cornea that associated to neurodegeneration results in vision loss, being this a devastating characteristic of the disease final stage. During disease progression, inflammatory molecules are produced and angiogenesis occur. Furthermore, VEGF is overexpressed by the maintained hyperglycemic environment and up‐regulated by tissue hypoxia. Also pro‐inflammatory mediators regulated by cytokines, such as tumor necrosis factor (TNF‐α) and interleukin‐1 beta (IL‐1β), and growth factors leads to the progression of these processes, culminating in vasopermeability (diabetes macular edema) and/or pathological angiogenesis (proliferative diabetic retinopathy). It was found a mutual contribution between inflammation and angiogenesis along the process. J. Cell. Biochem. 117: 2443–2453, 2016. © 2016 Wiley Periodicals, Inc.
Use of an Oral Busulfan Test Dose in Patients Undergoing Hematopoietic Stem Cell Transplantation Treated With or Without Fludarabine
Authors: de Castro Francine Attié , Simões Belinda Pinto , Godoy Ana Leonor Pardo Campos , Bertagnoli Trigo Fernanda Manuela , Coelho Eduardo Barbosa , Lanchote Vera Lucia 
Published: May 2016

AbstractThis study investigated the importance of an oral test dose for busulfan (BU) dose adjustment before a conditioning regimen for hematopoietic stem‐cell transplantation (HSCT) and the effect of fludarabine (FLU) on the oral BU pharmacokinetics evaluated after the fifth treatment dose (first BU dose on day 2 of treatment). Twenty‐eight patients treated with oral BU (1 mg/kg every 6 hours for 4 days) were divided into 2 groups according to the concomitant administration of FLU (n = 15; 30 mg/m2 for 5 days) or subsequent administration of cyclophosphamide (CY) (n = 13; 60 mg/kg for 2 days). On the day prior to the beginning of the conditioning regimen, blood samples were collected (0‐6 hours) after administration of an oral BU test dose of 0.25 mg/kg. Busulfan was quantified in plasma samples by LC‐MS/MS, and the pharmacokinetic parameters were calculated using WinNonlin software. Blood samples were collected between the fifth and sixth treatment dose to confirm the mean plasma steady‐state concentration (Css) of BU. The AUC0‐6 and apparent clearance of BU did not differ (P < .05) between the groups receiving FLU and CY. In 81% of the patients who received BU doses adjusted based on the test dose (n = 21), the Css was within the target range of 600‐900 ng/mL. No association was observed between BU AUC0‐6 and clinical outcome in the study group (n = 28). The results suggest that in concomitant administration of FLU and BU during conditioning regimens for HSCT, changes in BU dose should be considered only after the administration of the fifth BU dose.
Metabolic profiles of cow's blood; a review
Authors: Puppel Kamila , Kuczyńska Beata 
Published: May 2016

AbstractThe term ‘metabolic profile’ refers to the analysis of blood biochemical parameters that are useful to assess and prevent metabolic and nutritional disorders in dairy herds. In the higher standards of milk production, the priority in modern breeding is keeping dairy cows in high lactation and healthy. The proper analysis, as well as control. of their feeding and metabolic status is immensely important for the health condition of the herd. The disproportion between the genetically determined ability for milk production and the limitations in improving the energy value of the ration may be the cause of metabolic disorders. Negative energy balance has a major impact on the body's hormonal balance and organ functions and mostly appears during transition periods: from 3 to 2 weeks prepartum until 2–3 weeks postpartum. The term ‘transition’ is used to underscore the important physiological, metabolic and nutritional changes occurring in this time. The manner in which these changes occur and how they are diagnosed and detected are extremely important, as they are closely related to clinical and subclinical postpartum diseases, lactation and reproductive performance – factors that significantly shape the profitability of production. Therefore the priority for intensive milk production is prevention of metabolic diseases and other disorders. It is the intent of this review to synthesize and summarize the information currently available on metabolic status and physiological changes in the cow's body that occur during lactation, as well as to discuss the interpretation of the results, which will be a useful diagnostic tool in nutritional evaluations of the dairy herd. © 2016 Society of Chemical Industry
Sexual Health in Male and Female Iraq and Afghanistan U. S. War Veterans With and Without PTSD: Findings From the VALOR Cohort
Authors: Breyer Benjamin N. , Fang Shona C. , Seal Karen H. , Ranganathan Gayatri , Marx Brian P. , Keane Terence M. , Rosen Raymond C. 
Published: May 2016

AbstractWe sought to determine whether posttraumatic stress disorder (PTSD) was associated with sexual health in returned warzone‐deployed veterans from the recent Iraq and Afghanistan conflicts. We studied 1,581 males and females from the Veterans After‐Discharge Longitudinal Registry, a gender‐balanced U.S. Department of Veterans Affairs registry of health care‐seeking veterans with and without PTSD. Approximately one quarter (25.1%) of males (n = 198) and 12.7% of females (n = 101) had a sexual dysfunction diagnosis and/or prescription treatment for sexual dysfunction. Both genders were more likely to have a sexual dysfunction diagnosis and/or prescription treatment if they had PTSD compared with those without PTSD (male: 27.3% vs. 21.1%, p = .054; female: 14.9% vs. 9.4%, p = .022). Among the 1,557 subjects analyzed here, males with PTSD had similar levels of sexual activity compared to those without PTSD (71.2% vs. 75.4%, p = .22), whereas females with PTSD were less likely to be sexually active compared to females without PTSD (58.7% vs. 72.1%, p < .001). Participants with PTSD were also less likely to report sex‐life satisfaction (male: 27.6% vs. 46.0%, p < .001; female: 23.0% vs. 45.7%, p < .001) compared with those without PTSD. Although PTSD was not associated with sexual dysfunction after adjusting for confounding factors, it was significantly negatively associated with sex‐life satisfaction in female veterans with a prevalence ratio of .71, 95% confidence interval [.57, .90].
Edaravone protects against cobalt chloride‐induced dysfunctions in apoptosis and invasion in trophoblast cells
Authors: Zheng YanFang , Zhao Yin , Luo QingQing , Liu XiaoXia , Liu XiaoPing , Hu Ying , Zou Li 
Published: May 2016

SUMMARYThis study was performed to investigate the effects of edaravone on hypoxia‐induced trophoblast cell proliferation, apoptosis, and invasion. The trophoblast cell line HTR‐8 (H8) was treated with cobalt chloride (CoCl2) with or without a 1‐hr edaravone pretreatment, followed by assessment of intracellular reactive oxygen species (ROS) levels, cell proliferation, apoptosis, migration, and invasion. Metrics of apoptosis included measurement of cysteine‐aspartic acid protease 3 (CASP3) activity as well as BAX and BCL2 expression. Migration and invasion phenotypes were complemented with expression analysis of matrix metalloproteinase 2 (MMP2) and tissue inhibitor of metalloproteinase 2 (TIMP2) at the transcript and protein levels. CoCl2 inhibited the proliferation of H8 cells, promoted apoptosis, and up‐regulated CASP3 activation and BAX expression while inhibiting BCL2 expression. CoCl2 treatment also reduced the invasiveness of H8 cells by inhibiting MMP2 activity. Edaravone significantly increased H8 cell proliferation; inhibited apoptosis by down‐regulating CASP3 activation and BAX production while promoting BCL2 stability; and ameliorated the migration and invasion phenotypes associated with CoCl2 treatment. These results suggest that edaravone may rescue hypoxia‐induced abnormalities in H8 cell proliferation, apoptosis, and invasion, thereby protecting the trophoblast lineage against hypoxia. Mol. Reprod. Dev. 83: 576–587, 2016. © 2016 Wiley Periodicals, Inc.
Role of eNOS‐ and NOX‐containing microparticles in endothelial dysfunction in patients with obesity
Authors: Dimassi Saloua , Chahed Karim , Boumiza Soumaya , Canault Matthias , Tabka Zouhair , Laurant Pascal , Riva Catherine 
Published: May 2016

ObjectiveTo explore the pathophysiological profile of patients who have obesity and to investigate the potential role of circulating microparticles (MPs) in endothelial dysfunction in patients who have obesity.MethodsThe inflammatory and oxidative status and the cutaneous microvascular blood flow were characterized in 69 patients with android obesity and 46 subjects with normal weight (controls) by using laser Doppler flowmetry. Circulating MP levels were measured by flow cytometry, and endothelial nitric oxide synthase (eNOS) and NADPH oxidase (NOX) expression in MPs was investigated by Western blotting. MP effect on vascular reactivity was assessed in rat aorta rings.ResultsPatients with obesity showed endothelial dysfunction, hyperglycemia, inflammation, and oxidative stress. In controls, low MP levels were positively correlated with normal microvascular function. Western blot analysis revealed reduced eNOS and increased NOX4D expression in MPs from subjects with obesity compared with controls. However, this was not correlated with endothelial dysfunction parameters and did not impair ex vivo endothelium‐dependent vasodilation.ConclusionsThese results suggest that MPs do not contribute directly to endothelial dysfunction associated with obesity. Conversely, eNOS‐ and NOX‐containing MPs could be involved in the compensatory mechanism of vascular endothelial cells to counteract the pathologic mechanisms underlying endothelial dysfunction.
Assessing the Prognostic Significance of Histologic Response in Osteosarcoma: A Comparison of Outcomes on CCG‐782 and INT0133—A Report From the Children's Oncology Group Bone Tumor Committee
Authors: Bishop Michael W. , Chang Yu‐Chen , Krailo Mark D. , Meyers Paul A. , Provisor Arthur J. , Schwartz Cindy L. , Marina Neyssa M. , Teot Lisa A. , Gebhardt Mark C. , Gorlick Richard , Janeway Katherine A. , Chou Alexander J. 
Published: May 2016

BackgroundThe prognostic value of histologic response for osteosarcoma may have changed with induction chemotherapy schedules over time. We hypothesized that the increased intensity of induction therapy provided on INT0133 compared to the Children's Cancer Group study CCG‐782 would diminish the impact of histologic response on the risk of events after definitive surgery.MethodsRetrospective analysis was performed for patients aged <22 with newly diagnosed nonmetastatic osteosarcoma enrolled on CCG‐782 and INT0133. Clinical factors were evaluated for association with response and outcome. Good response was defined as <5% viable tumor at resection. Associations of response, study, and postdefinitive surgery event‐free survival (EFS‐DS) were determined using Cox proportional hazard models. EFS‐DS was estimated by Kaplan–Meier methodology.ResultsData were available for 814 patients (206 CCG‐782, 608 INT0133). For good responders, 10‐year EFS‐DS (±SE) was 75.4% ± 7.7% for CCG‐782 and 70.8% ± 3.1% for INT0133. For poor responders, 10‐year EFS‐DS was 39.9% ± 4.9% for CCG‐782 and 58.4% ± 3.1% for INT0133. Histologic response predicted outcome across studies (P < 0.0001). Significant interaction between study and histologic response was observed for EFS‐DS (P = 0.011). Using proportional hazards regression, INT0133 poor responders had less risk of events compared to CCG‐782 poor responders (relative hazard ratio (RHR) = 0.6:1), but good responders on INT0133 had a greater risk of events compared to CCG‐782 good responders (RHR = 1.53:1).ConclusionWe observed an inverse relationship between the predictive value of tumor necrosis and intensity of induction therapy, raising questions about the true prognostic value of histologic response. This highlights the need for novel markers to develop strategies for treatment in future trials.
Chromosomal microarray use among women undergoing invasive prenatal diagnosis
Authors: Naqvi Mariam , Goldfarb Ilona T. , Hanmer Kaitlin J. , Bryant Allison 
Published: May 2016

AbstractObjectiveTo study the offer and uptake of chromosomal microarray analysis (CMA) among women undergoing invasive prenatal testing.MethodsThis is a retrospective cohort study of women who underwent chorionic villus sampling (CVS) or amniocentesis. Charts were reviewed for CMA offer and uptake, in additional to clinical and demographic variables.ResultsOne hundred forty‐one women underwent CVS (n = 53) or amniocentesis (n = 91) over the study period. Overall, 41% of women underwent CMA. Women who underwent invasive testing for a fetal structural abnormality were more likely to undergo CMA than women who underwent invasive testing for all other indications (aOR 43.18, 95% CI 4.64 – 401.58). Chromosomal microarray was offered more often to women who primarily spoke English (p < 0.001), self‐identified as white (p = 0.046) and did not receive prenatal care in a community health center (p = 0.044). Statistically significant differences in CMA uptake by race/ethnicity, language, insurance or provider type were not noted. Multiparous women were less likely to accept this test than nulliparas (aOR 0.39, 95% CI 0.17 – 0.86).ConclusionWomen who undergo invasive fetal testing are more likely to undergo CMA if the indication is for a fetal structural anomaly. There may be important demographic disparities in the offering of CMA which bear further exploration. © 2016 John Wiley & Sons, Ltd.
Impact of Isotope Dilution Mass Spectrometry (IDMS) Standardization on Carboplatin Dose and Adverse Events
Authors: Lawson Justin , Switchenko Jeffrey M. , McKibbin Trevor , Donald Harvey R. 
Published: May 2016

BackgroundWhen using area under the concentration–time curve–based strategies for dosing carboplatin, accurate estimation of glomerular filtration rate is required for determining dose. Commonly, the Cockcroft–Gault equation is used, which is dependent on measurement of serum creatinine (SCr). Because analysis of SCr changed to an isotope dilution mass spectrometry (IDMS) standard, we sought to determine the impact of this assay change on carboplatin dosing and related toxicity.MethodsThis was a single‐center, retrospective chart review of adults treated with carboplatin between April 2008 and April 2010 divided into cohorts that initiated carboplatin before or after IDMS standardization. End points included grade 3 thrombocytopenia, decrease in platelet count, and hospitalization and were evaluated in cohorts based on concomitant chemotherapy.ResultsThe chart review identified 158 patients, with 63 patients in the pre‐IDMS group and 95 patients in the post‐IDMS group. Average SCr (pre 1.01 mg/dl vs post 0.86 mg/dl, p<0.001) and average carboplatin dose (pre 580 mg vs post 703 mg, p<0.001) were significantly different between the groups. The frequency of grade 3 thrombocytopenia was not statistically significant across three partner chemotherapy cohorts before and after IDMS implementation.ConclusionIDMS standardization led to an overall decrease in SCr with subsequent increase in carboplatin doses. However, no increase in recorded adverse events was observed, suggesting that the clinical relevance in toxicity from higher doses was minimal.
Immobilized metal affinity chromatography optimized for the analysis of extracellular phosphorylation
Authors: Klement Eva , Raffai Timea , Medzihradszky Katalin F. 
Published: May 2016

Phosphorylation is the most widely studied posttranslational modification. Its role within the cell has been the focus of numerous large‐scale studies. Recently there is growing evidence on the biological significance of extracellular phosphorylation. The analysis of these phosphopeptides is complicated by the abundance of glycosylation in the extracellular space, since glycopeptides are also enriched by the methods used for phosphopeptide isolation. Thus, we optimized IMAC for phosphorylation analysis of secreted proteins, specifically in human serum. Selectivity and efficiency of different enrichment conditions used in earlier large‐scale phosphoproteomic studies were evaluated. We found that minimizing hydrophilic interactions in the enrichment allowed selective phosphopeptide isolation. Using a two‐step IMAC enrichment protocol under these conditions led to the identification of ∼100 phosphorylation sites from the tryptic digest of as little as 40 μL human serum.
Post‐functionalization of perfluorophenyl ester‐functional acyclic diene metathesis polymer
Authors: Daglar Ozgun , Durmaz Hakan , Hizal Gurkan , Tunca Umit 
Published: May 2016

ABSTRACTThe acyclic diene metathesis (ADMET) polymerization was utilized for the design of the ADMET polymer (Mn = 21,200 g/mol, Mw/Mn = 1.74) with pendant perfluorophenyl ester functionality using Grubbs first generation catalysis overnight in bulk at 80 °C. Next, a wide variety of functional groups, like benzyl, octyl, propargyl, allyl, and furfuryl was quantitatively incorporated to the ADMET polymer backbone through various amines using activated ester substitution reaction. The ADMET polymers studied in this work were characterized using 1H, 13C, and 19F NMR, GPC and DSC and displayed a monomodal distribution and a rather broad polydispersity index in the range of −1.33 to 1.90. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2593–2598