bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐port laparoscopic reversal of Hartmann's procedure through the colostomy site: technical aspects and early postoperative outcomes
Authors: Akmercan Ahmet , Akmercan Tayfun , Uprak Tevfik Kıvılcım 
Published: October 2024

AbstractBackgroundSingle‐port laparoscopic surgical approaches offer improved cosmetic outcomes and enhance recovery following surgery. The purpose of the study was to assess the reliability and efficacy of a single‐port laparoscopic Hartmann's reversal(SPL‐HR) through the colostomy site.MethodsProspective data from consecutive patients who underwent SPL‐HR between 2020 and 2024 was analysed. Once the colostomy was detached from abdominal wall, a single‐port device was introduced through the colostomy site. After mobilizing the rectal stump and afferent colon, colorectal anastomosis was carried out using a circular stapler transanally under laparoscopic vision. Postoperative care and discharge decisions were made following the ERAS protocol. Patient demographics, details of Hartmann's procedure, intraoperative outcomes, and early postoperative outcomes were evaluated.ResultsSPL‐HR was successfully performed in 23 of 27 patients (85.1%), with a median operation time of 92 (50–172) min and a median blood loss of 100 (10–360) mL. Five patients (21.7%) experienced a postoperative complication. Two of them experienced grade 3a complications according to Clavien‐Dindo classification: one with an intraabdominal abscess and one with a hematoma at the colostomy site, both requiring drainage under local anaesthesia. Anastomotic leak or mortality wasn't observed in patients. Functional recovery measures such as first flatus time and time to resuming a soft diet were favourable, with a median length of hospital stay of 4 (2–9) days.ConclusionThe SPL‐HR technique is a reliable and efficient method that is easy to perform. It has acceptable complication rates while improving postoperative recovery and reducing the length of hospital stay.
Metabolic risk factor targets in relation to clinical characteristics and comorbidities among individuals with type 2 diabetes treated in primary care – The countrywide cross‐sectional AUSTRO‐PROFIT study
Authors: Sourij Harald , Azhar Kehkishan , Aziz Faisal , Kojzar Harald , Sourij Caren , Fasching Peter , Clodi Martin , Ludvik Bernhard , Mader Julia K. , Resl Michael , Rega‐Kaun Gersina , Ress Claudia , Stechemesser Lars , Stingl Harald , Tripolt Norbert J. , Wascher Thomas , Kaser Susanne 
Published: October 2024

AbstractAimsThis study assessed the achievement rates of metabolic risk factor targets and their association with clinical characteristics and comorbidities among individuals with type 2 diabetes (T2D) treated in the primary care in Austria.Materials and MethodsA countrywide cross‐sectional study, the AUSTRO‐PROFIT, was conducted in Austria from 2021 to 2023 on 635 individuals with T2D. Metabolic risk factor targets were defined as the percentage of people achieving low‐density lipoprotein cholesterol (LDL‐C) <70 mg/dL (or < 55 mg/dL if cardiovascular or microvascular disease was present), glycated haemoglobin (HbA1c) <7% (53 mmol/mol) and blood pressure < 140/90 mmHg.ResultsThe mean age of the participants was 65.7 ± 11.2 years; the median duration of T2D was 8 (4–14) years; and 58.7% of the participants were male. The percentages of participants achieving LDL‐C, HbA1c, blood pressure and all targets were 44%, 53%, 57% and 13%, respectively. Older age, longer T2D duration, cardiovascular disease and microvascular complications were associated with suboptimal achievement of metabolic risk factor targets.ConclusionsThe AUSTRO‐PROFIT study revealed notable variations in metabolic targets achievement with respect to clinical characteristics and comorbidities. These findings underscore the importance of establishing national diabetes registries and implementing multifactorial targeted and individualized interventions to further improve the quality of T2D care in primary care settings in Austria.
Roles of ZEB1 and ZEB2 in E‐cadherin expression and cell aggressiveness in head and neck cancer
Authors: Kinouchi Arisa , Jubashi Takahiro , Tatsuno Rikito , Ichikawa Jiro , Sakamoto Kaname , Sakurai Daiju , Kawasaki Tomonori , Ishii Hiroki , Miyazawa Keiji , Saitoh Masao 
Published: October 2024

AbstractZinc finger E‐box binding homeobox 1 (ZEB1) has been identified as a key factor in cancer cell differentiation and metastasis, and has been well studied in the field of cancer cell biology. ZEB2 has a highly similar conformation to ZEB1, but its role in head and neck squamous cell carcinoma (HNSCC) cells is not fully understood. Here, we separately overexpressed ZEB1 and ZEB2 in C57BL/6 mouse oral cancer (MOC) cells and investigated their cellular characteristics, including E‐cadherin levels, motile properties, chemoresistance, and metastatic ability in immunocompetent mice. Both ZEB1 and ZEB2 overexpression reduced epithelial traits and converted cells to an aggressive phenotype. Surprisingly, ZEB1 overexpression increased the endogenous level of ZEB2 in MOC cells, and vice versa. The molecular mechanisms underlying these findings remain unclear. However, the in vitro anchorage‐independent growth of MOC cells overexpressing ZEB2 was considerably greater than that of MOC cells overexpressing ZEB1. These findings suggest that ZEB2, like ZEB1, has the ability to induce the differentiation of cancer cells into those with highly aggressive traits.
A trifecta trajectory of moral taint contagion: Women (church) leaders making work dirty
Authors: Grandy Gina , Mavin Sharon 
Published: October 2024

AbstractThis article offers insights into the dynamic and complex nature of moral taint, specifically taint that emanates at the individual level, triggered by gender, and the trajectory of this moral taint at work. Through a qualitative study of women church leaders, it explores how gender might serve as a trigger of moral taint to dirty the work. The women church leaders are perceived to be morally questionable and a source of disgust. Their gender triggers moral taint. This moral taint creates the risk of a reverse trifecta of taint contagion from the women toward the occupation, institution, and stakeholders. Others respond to the women with disgust, deflecting and protecting against contamination. The women leaders perform gendered dirty work in this sacredly masculine context and their leadership privilege is unstable, immoral, and contested because of their gender. We offer a conceptualization of gendered dirty work(ers) and suggest research avenues for women in leadership.
Potential and application of tandem mass spectrometry (MS/MS) in the analysis and identification of novel bacteriocins: a review
Authors: Kurnianto Muhammad Alfid , Adesina Precious Adedayo , Rini Dina Mustika 
Published: October 2024

SummaryBacteriocins are antimicrobial peptides synthesised ribosomally by Gram‐positive or Gram‐negative bacteria to gain a competitive advantage. The majority of bacteriocins are derived from Gram‐positive bacteria, with lactic acid bacteria being the most common source. Because they are considered ‘natural’, there is currently significant development of bacteriocins for application as food preservative agents. As a preservative agent, bacteriocin activity is highly dependent on purity, down to the amino acid profile and sequence. Therefore, bacteriocin identification is important. Currently, MS is a cutting‐edge tool in bacteriocin identification. This method has high selectivity, sensitivity and resolution. To the best of our knowledge, systematic reviews focusing on the application of MS for bacteriocin identification are currently limited. In light of this, the objective of this study is to provide a comprehensive review and summary of MS technologies in bacteriocin research, with a particular focus on the discovery and characterisation of novel sources of bacteriocin. Additionally, studies related to the discovery of bacteriocins from various sources, their role as antimicrobial agents, and their synthesis are emphasised. Thus, this study presents a comprehensive analysis of the advantages, limitations, and future perspectives of the methods employed.
The potential of oviduct tags and fine‐scale acoustic telemetry to reveal the timing and location of spawning in Arctic salmonids (Salvelinus spp.)
Authors: Dubos Véronique , Harris Les N. , Ekpakohak Richard , Malley Brendan K. , Gilbert Matthew J. H. , Furey Nathan B. , Moore Jean‐Sébastien 
Published: October 2024

AbstractIdentifying and characterizing spawning locations are paramount for the protection of critical fish habitats but can be challenging, particularly in remote locations. Using the underexplored oviduct‐tagging technique, we aimed to identify the timing and location of spawning for wild Arctic char (Salvelinus alpinus) and lake trout (Salvelinus namaycush) in two high‐Arctic lakes in Nunavut. Specifically, Innovasea V7 acoustic telemetry transmitters were inserted into the oviducts of 13 Arctic char and 4 lake trout, and the timing and location of tag expulsion were determined using a fine‐scale positioning system. Twenty Arctic char and 20 lake trout were also tagged with abdominal V16 transmitters, and 10 of them were paired with the oviduct tags, to further study the behavior of individual fish during the spawning season. Oviduct tags from four Arctic char and one lake trout could be used to assess the timing and location of spawning. Spawning anadromous Arctic char drastically reduced their activity and remained proximate to their presumed spawning location immediately before and for months after spawning. In contrast, a non‐anadromous (i.e., freshwater resident) Arctic char and a lake trout showed little to no reduction in activity around presumed spawning events. Because of the highlighted sedentary behavior of inferred spawning anadromous Arctic char implanted with both abdominal and oviduct tags, we could also infer potential spawning based on the behavior of individuals equipped only with abdominal tags. Spawning areas identified via telemetry also aligned well with Inuit knowledge of those lakes. This is the first field study to use acoustic oviduct and abdominal tags coupled with a fine‐scale positioning system. Despite a limited success rate of ejection, the study reveals the strong potential of the method to study spawning habitat and timing, particularly in remote areas.
Clinical Utility of Genetic Variants in PNPLA3 and TM6SF2 to Predict Liver‐Related Events in Metabolic Dysfunction‐Associated Steatotic Liver Disease
Authors: Seko Yuya , Yamaguchi Kanji , Shima Toshihide , Iwaki Michihiro , Takahashi Hirokazu , Kawanaka Miwa , Tanaka Saiyu , Mitsumoto Yasuhide , Yoneda Masato , Nakajima Atsushi , Okanoue Takeshi , Itoh Yoshito 
Published: October 2024

ABSTRACTBackground and AimsFibrosis‐4 (FIB‐4) index and genetic polymorphisms have been used in assessing the risk of liver‐related events (LRE) in metabolic dysfunction‐associated steatotic liver disease (MASLD). To establish a more efficient prediction strategy for LRE, we investigated a combined approach that uses the FIB‐4 index and genetic polymorphisms.MethodsWe enrolled 1304 Japanese patients with biopsy‐proven MASLD in this longitudinal multicenter cohort study. PNPLA3, TM6SF2, GCKR and MBOAT7 genotypes were genotyped, and polygenic risk score high fat content (PRS‐HFC) were calculated.ResultsDuring the follow‐up period of 8.1 year, 96 LRE occurred and 53 patients died. PNPLA3, TM6SF2 and GCKR genotypes were associated with LRE development. We divided patients into three groups based on the FIB‐4 index and PNPLA3 and TM6SF2 genotype. The cumulative LRE development rate in each group was 2.1%/28.9%/53.5%, respectively, at 10 years. Multivariate analysis revealed hazard ratios (HRs) for LRE of 10.72 in the high‐risk group and 4.80 in the intermediate‐risk group. Overall survival in each group was 98.8%/85.2%/72.4%, respectively, at 10 years. HRs for prognosis were 8.74 in the high‐risk group and 5.62 in the intermediate‐risk group. Patients with FIB‐4 index > 2.67 and high PRS‐HFC had HR of 6.70 for LRE development and HR of 6.07 for prognosis compared to patients with FIB‐4 ≤ 2.67.ConclusionsThe approach of measuring the FIB‐4 index first followed by assessment of genetic polymorphisms efficiently detected patients at high risk of developing LRE. Therefore, this two‐step strategy could be used as a screening method in large populations of patients with MASLD.
Nomogram to Predict Nodal Recurrence‐Free Survival in Early Oral Squamous Cell Carcinoma
Authors: Liu Ying , Liu Limin , He Yining , Jiang Wen , Fang Tianyi , Huang Yingying , Zhou Xinyu , Zhu Dongwang , Li Jiang , Zhong Laiping 
Published: October 2024

ABSTRACTObjectiveThis study aimed to develop and internally validate a prognostic nomogram for predicting nodal recurrence‐free survival (NRFS) in patients with early‐stage oral squamous cell carcinoma (OSCC) with clinically negative neck lymph nodes.Materials and MethodsThe management of early‐stage oral cancer patients with clinically negative neck lymph nodes (cN0) remains controversial, especially concerning the need for elective neck dissection. Data from a single institution spanning 2010 to 2020 were utilized to develop and evaluate the nomogram. The nomogram was constructed using multivariable Cox regression and LASSO regression analyses to identify independent risk factors for lymph node metastasis. Internal validation was performed using bootstrap resampling to assess the nomogram's predictive accuracy.ResultsA total of 930 cN0 patients with T1 and T2 stage OSCC were randomly divided into training and validation cohorts (8:2 ratio). Independent risk factors for lymph node metastasis included tumor pathological grade (well: reference, moderate/poor: OR 1.69), cT (cT1: reference, cT2: OR 2.01), history of drinking (never: reference, current/former: OR 1.72), and depth of invasion (0 mm < DOI ≤ 5 mm: reference, 5 mm < DOI ≤ 10 mm: OR 1.31). The nomogram, incorporating these variables, demonstrated good predictive accuracy with a C‐index of 0.67 (95% CI: 0.58–0.76) in the validation set. In both training and validation groups, the nomogram effectively stratified patients into low‐risk and high‐risk groups for occult cervical nodal metastases (p < 0.05).ConclusionsThe nomogram enables risk stratification and improved identification of occult cervical nodal metastases in clinically node‐negative OSCC patients by incorporating tumor‐specific and patient‐specific risk factors.
Gender, BMI, and Age‐Related Variations in Lower Limb Alignment Parameters and CPAK Phenotypes in Chinese Patients with Knee Osteoarthritis
Authors: Li Songlin , Chen Xi , Liu Sen , Xu Hongjun , Yu Yang , Li Shanni , Yin Zhaojing , Du Yiyang , Zhang Miaomiao , Liu Peilai , Qian Wenwei 
Published: October 2024

ObjectivesResearch on the distribution of and the variation in coronal plane alignment of the knee (CPAK) in the Chinese osteoarthritis population is limited. We aimed to establish the CPAK classification based on the characteristics of lower limb alignment in the Chinese osteoarthritis population. We also investigated variations in lower limb alignment parameters and CPAK phenotypes based on gender, body mass index (BMI), and age.MethodsA retrospective study was conducted on a total of 944 knees diagnosed with osteoarthritis in 479 patients from January 2017 to December 2023. A scatterplot was used to describe the distribution of the CPAK classification, and the differences in lower limb alignment parameters and the CPAK classification were compared across genders (male, female), ages (middle‐aged/<65 years, elderly/≥65 years), and BMI categories (normal/<25 kg/m2, overweight and obese/≥25 kg/m2) using the chi‐squared test or Fisher's exact test.ResultsThe average arithmetic hip‐knee‐ankle angle and joint line obliquity (JLO) were −3.03° ± 5.69° and 174.45° ± 4.29°, respectively. There was a higher prevalence of constitutional varus alignment in males and the overweight or obese group, while constitutional valgus alignment was more common in females and the normal BMI group (p < 0.05). Additionally, females had a greater apex distal JLO than males (p < 0.05). There were no statistically significant differences in lower limb alignment parameters among different age groups (p > 0.05). Although there were variations in alignment parameters across different genders and BMI categories in the knee osteoarthritis population, the predominant CPAK classifications were type I (38.03%), followed by type II (20.02%) and type IV (17.06%).ConclusionThe most common CPAK types were I, II, and IV, and they were not influenced by gender, BMI, or age, indicating that the CPAK classification can reliably reflect constitutional alignment. A better understanding of native alignment variability can aid in providing patient‐specific recommendations when considering orthopedic alignment strategies.
Evaluation of propofol‐based procedural sedation in pediatric hemato‐oncological patients provided by physician assistants in anesthesia
Authors: van Langeveld I. , Aalbers T. , Prudon I. , Koning R. , Dors N. , Hampsink B. , Bronkhorst E. M. , Scheffer G. J. , Malagon I. , Hendriks M. 
Published: October 2024

AbstractBackgroundProcedural sedation and analgesia are required for painful hemato‐oncological procedures such as lumbar and bone marrow punctures. At our institution, sedation with propofol and alfentanil is usually provided by Physician Assistants in Anesthesia. We evaluated the adverse events during the PSA program for children, provided by Physician Assistants in Anesthesia.Patients and MethodsWe included pediatric patients meeting our criteria for deep sedation by a Physician Assistants in Anesthesia, scheduled for a hemato‐oncological procedure at the Amalia Children's Hospital at the Radboudumc Nijmegen. The primary outcome was oxygen desaturation below 92% for more than 20 s. We prospectively collected data on demographics, current health problems, type of procedure, need for airway interventions, and hypotension.ResultsWe collected data from 437 sedation sessions involving 71 patients. No oxygen desaturation below 92% lasting longer than 20 s occurred. In 2 cases, a jaw thrust was performed. No invasive airway techniques (oropharyngeal cannula, laryngeal mask, or intubation) were required. A significant drop in mean arterial pressure was seen in 2 out of 437 cases (0.5%). There was no occurrence of cardiopulmonary resuscitation or other adverse events such as aspiration or laryngeal spasm.ConclusionsSedation and analgesia for short painful procedures in selected pediatric hemato‐oncological units with a dedicated protocol may be safely provided by trained and certified Physician Assistants in Anesthesia.
The Dogmatism Puzzle Undone
Authors: Simpson James 
Published: October 2024

ABSTRACTAccording to the dogmatism puzzle, for any S and any p, if S knows that p, then she is entitled to be dogmatic about p, and so disregard any evidence against p, for she knows that (or is in a position to know that) that evidence is misleading. But this seems clearly problematically dogmatic. The standard solution to the dogmatism puzzle involves appealing to the view that acquiring new evidence (even misleading evidence) can undermine one's knowledge that p. That is why one cannot rightly disregard any future evidence against p. This solution to the dogmatism puzzle has come to be called “the defeat solution.” Maria Lasonen‐Aarnio has recently argued, however, that the defeat solution leaves unsolved a partial defeat version of the dogmatism puzzle, where some subject acquires weak misleading evidence against p, but, since it is weak, it does not rob her of knowledge that p. Lasonen‐Aarnio argues that solving this partial defeat version of the dogmatism puzzle requires those who endorse the defeasibility of knowledge to either go dogmatist or reject an extremely plausible principle that she calls “Entitlement” (roughly, for any S and any e, if S knows that evidence e is misleading, then S can rightly disregard e). In this paper, however, I argue that defeasibilists face no such challenge from any version of the dogmatism puzzle, since the dogmatism puzzle, in both its original and partial defeat form, rests on an assumption that we have very good reason to think is mistaken. Specifically, the assumption that, for any S and any p, if S knows that p, then S knows (or is in a position to know) that any evidence against p is misleading. I further argue that rejecting this assumption also yields a neat solution to the dogmatism puzzle involving intention originally proposed by Saul Kripke and recently adapted by R.E. Fraser.
RETRACTED: Overexpression of SEZ6L2 and Immune Infiltration in Cancer Based on Gene Image Diagnosis
Authors: Ding Liangfu , Zeng Jilin , Zhao Junyong 
Published: October 2024

ABSTRACTBackgroundWith the rapid advancement of optical image diagnostic technology, researchers are delving into the potential applications in the field of cancer diagnosis and treatment. The exact link between the SEZ6L2 gene and cancer immune infiltration remains elusive.Materials and MethodsThis study aims to investigate the relationship between SEZ6L2 gene overexpression and cancer immune infiltration using optical image diagnostic technology, thereby presenting novel insights for enhancing cancer diagnosis and treatment strategies. Tissue samples obtained from cancer patients were meticulously analyzed to quantitatively assess the expression of the SEZ6L2 gene through light image diagnostic technology. Additionally, immunohistochemical techniques were employed to assess the nature and quantity of immune infiltrating cells within the cancerous tissues.ResultsThe enrichment pathways were found to include complement activation, circulating immunoglobulin mediated humoral immune response, protein activation cascade, immunoglobulin complex, and immunoglobulin. In addition, the expression of SEZ6L2 is closely related to the infiltration level of tumor infiltrating immune cells (TIICs), and there is a potential relationship between the expression of SEZ6L2 and different marker genes of TIIC.ConclusionIncreased SEZ6L2 mRNA expression in breast invasive carcinoma was significantly associated with negative prognosis and immune invasion. SEZ6L2 may be a novel prognostic biomarker and a potential immunotherapeutic target in BRCA.