bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of RSPO3 rearrangements in advanced colorectal cancer patients detected by NGS and their relationship with disease characteristics
Authors: Tur Raquel , Abad Mar , Filipovich Elena , Rivas María Belén , Rodríguez Marta , Montero Juan Carlos , Sayagués José María 
Published: October 2024

Abstract

Colorectal cancer (CRC) is the second most frequent cause of cancer-related deaths. Mortality is largely due to limited treatment options for patients who present with advanced disease. Genomic abnormalities that are potentially characteristic of such advanced stages of the disease are complex and are not yet fully understood. In fact, only 30% of cases with advanced disease benefit from targeted treatments. The molecular heterogeneity of primary advanced CRC (aCRC) tumor samples was evaluated by next-generation sequencing (NGS) in 53 consecutive patients (pT4a-b). The genetic abnormalities found in primary tumors were most frequently mutations in TP53 (57% of cases), KRAS (45%), PI3KCA (27%), BRAF (15%) and RET (10%), as well as RSP03 fusions (8%). Alterations in the TP53 and NRAS genes were more commonly observed in the left colon, while BRAF mutations and RSPO3 fusions were more frequently detected in the right or transverse colon. We also show a strong association between the presence of RSPO3 rearrangements and patients with small, low histological grade tumors, with normal CEA levels, preferentially located on the right side of the colon. Furthermore, aCRC patients with PTPRK::RSPO3 fusions showed a higher frequency of deaths, and RSPO3 gene expression levels had an adverse impact on overall survival in two large, independent series of patients with CRC. We show that RSPO3 rearrangements occur very frequently in advanced disease, making it a promising therapeutic target for this subtype of patient.
Patient Satisfaction towards Telemedicine Services at the Satellite Centre of Tribal Health and Research in district Sirohi Rajasthan-A Mixed Method Study.
Authors: Dwivedi Rakhi , Jain Ridhi , Goyal Prachi , Goel Akhil Dhanesh , Bohra Gopal Krishna , Bhardwaj Abhishek , Singh Pratibha , Singh Kuldeep , Dwivedi Pradeep 
Published: October 2024

Abstract

Objective: This mixed-method study evaluates patient satisfaction towards telemedicine services at the Satellite Centre for Tribal Health and Research (STHR) in district Sirohi, Rajasthan.
Background: Telemedicine delivers remote medical care using technology, improving healthcare access for marginalised communities in tribal areas. This telemedicine centre at STHR aimed to provide high-quality healthcare services through telemedicine to the tribal population in the region.
Methods: Conducted from February 6, 2022, to May 31, 2024, the study involved 4570 tribal patients, with 3704 (81%) responding to a satisfaction survey using Telehealth Satisfaction Survey (TeSS) rated on a 3-point Likert scale. Quantitative data were analyzed using SPSS version 22, and qualitative data were collected via open-ended questionnaires and focus group discussions.
Results: The median age of respondents was 32 years, with 58.6% being male. Satisfaction scores ranged from 2.00 for voice quality to 2.31 for the courtesy of telemedicine staff, indicating a generally high satisfaction level. Thematic analysis identified key themes such as convenience, accessibility, and communication, despite challenges like internet connectivity issues.
Conclusion: Healthcare service satisfaction among patients helps in building and maintaining sustainable services and is also one of the key factors for service utilization. The telemedicine initiative at Satellite Centre for Tribal Health and Research has high satisfaction levels that underscore the potential of telemedicine to meet the healthcare needs of marginalized communities, provided technological and communication barriers are addressed.
Association between 5-minute Oxygen Saturation and Neonatal Death and Intraventricular Hemorrhage among Extremely Preterm Infants
Authors: Lee Henry , Jiang Siyuan , Cui Xin , Katheria Anup , Finer Neiil , Bennett Mihoko 
Published: October 2024

Abstract

Objective
To assess the relationship between 5-minute oxygen saturation (SaO₂) and outcomes in extremely preterm infants.
Study Design:
This cohort study included infants ≤ 28 weeks' gestation across nine hospitals from 2020 to 2022. Death and / or severe intraventricular hemorrhage (IVH) were compared between infants with 5-minute SaO2 < 80% and 80–100% using Poisson regression models. Two-piecewise segmented regression model was used to estimate breakpoint of 5-minute SaO₂ in relation to outcomes.
Result
Of 390 infants, 184 (47.2%) had 5-minute SaO₂ < 80%. A 5-minute SaO₂ < 80% was independently associated with increased risks of death and / or severe IVH, early death, and severe IVH. Analysis of SaO₂ as a continuous variable revealed a breakpoint at 80% (95% CI: 75–86%), above which no additional benefit in outcomes was observed.
Conclusion
Our findings support the current recommendation of 5-minute SaO₂ target of 80% for extremely preterm infants.
Acanthosis Nigricans as a Beacon for Hepatitis C
Authors: George Abe , Kumar Sumir 
Published: October 2024

Abstract

Background: Acanthosis nigricans (AN) is a dermatological condition often associated with insulin resistance and obesity. This case report presents an unusual occurrence of AN in a non-obese patient with newly diagnosed hepatitis C virus (HCV) infection.
Case Report: A 40-year-old male presented with dark, velvety skin patches on his face, neck, axillae, and groins. Clinical and histopathological examination confirmed AN. Laboratory investigations revealed HCV infection and indicators of insulin resistance.
Conclusion: This case strengthens the previous observations and uniquely suggests a potential association between HCV infection, insulin resistance, and the development of AN, highlighting the need for further research into this relationship.
Persistent oral health inequality in children—repeated cross-sectional studies in 2010 and 2019
Authors: Blomma Caroline , Davidson Thomas , Gerdin Elisabeth Wärnberg , Bågesund Mats , Lyth Johan 
Published: October 2024

Abstract

Background and aim: Children growing up in vulnerable circumstances have a higher risk of caries experience. Tracking the development of caries in relation to socioeconomic variables over time is essential for fair resource distribution to groups with higher caries risk and to even out inequalities in oral health. The aim was therefore to analyse the association between 6-year-olds´ caries prevalence and socioeconomic variables at family and residential area levels in 2010 and 2019 and the trends in these associations.
Methods: The study design is an epidemiological registry-based, repeated cross-sectional study based on caries data (grouped as 0, 1–3 and >3 dmft) for the population of 6-year-olds in 2010 (n =4,408, 95% coverage) and 2019 (n =5,199, 94% coverage) in a Swedish region. Multiple socioeconomic variables for the children’s families and residential areas were retrieved from official registries. Multinomial logistic regression was performed at both levels to produce models for each level and studied year.
Results: The variables that explained most of the association between caries and socioeconomic variables were mainly the same over the years at both levels. At the family level, these were: maternal age when having their first child (explained most of the association in both years, at 30 and 35%, respectively); maternal age when having the child in the study group; parental employment; parental and child’s migration background; maternal educational level; form of housing; and financial assistance (only 2010). At the residential area level, these were: migration background (explained most of the association both years; 82 and 52 %, respectively), educational level and number of persons per household. The association between socioeconomic variables and caries was consistently stronger for severe caries (dmft >3) than moderate (dmft 1–3). Multiple socioeconomic risk variables meant even greater likelihood of caries.
Conclusion: Over the studied years, variables related with socioeconomics vulnerability continued to be associated with caries in young children growing up under socially disadvantaged circumstances. Effective efforts for families living in socially vulnerable contexts are needed to achieve good and equal oral health, as is continued follow-up to evaluate whether the goal is reached.
Obervation of subrelativistic collisionless shocks generation and breakout dynamics
Authors: Tian Ye , Bai Yafeng , Zhang Dongdong , Zeng Yushan , mao jiakang , Song Liwei , Li Ruxin 
Published: October 2024

Abstract

Relativistic collisionless shocks1, which are ubiquitous in the cosmos, play a significant role in various astrophysical phenomena such as gamma-ray bursts2–4, PeVatrons5–7, and shock breakouts accompanying a supernova explosion8. To unravel these processes happening at enormous spatial and energy scales, the most convenient and feasible approach so far – laser – has been long sought to access such extreme conditions in an ultrafast and volume-efficient manner9–13. Yet observing a relativistic collisionless shock in a laboratory has remained out of reach. Here, we demonstrate that sub-relativistic collisionless shocks with velocities ~ 0.03c of astrophysical significance can be produced and characterized in a laboratory using a table-top femtosecond “laser engine”. We attribute the shock formation to a rapidly growing Weibel instability in a carefully adjusted low-density preplasma environment, which resembles the interstellar media near an astrophysical central engine. Owing to this Weibel instability, a magnetic field as high as ~ 5000 T is developed within 2.7 ps, leading to the formation of a collisionless shock and shock breakout is finally observed at the preplasma edge. Our results not only pave the way for further exploration of astrophysics related to relativistic collisionless shocks but also enable a new regime of ultrahigh magnetic field physics on a tabletop scheme14.
Short sleep duration as a risk factor for cataract in the elderly hypertensive patients in rural China
Authors: Yang Dongbin , Li Chen , Ma Mingze , Xue Yunhui , Guo Xinghong , Jia Shiyu , Zhao Lipei , Tarimo Clifford Silver , Dai Nengguang , Liu Rongmei , Zhao Qiuping , Miao Yudong , Wu Jian , Ye Beizhu 
Published: October 2024

Abstract

Background
In an aging society, cataracts continue to significantly impact the quality of life for an increasing number of elderly individuals. As a risk factor for cataract, hypertension is becoming increasingly prevalent among the elderly year by year. The association between sleep duration and cataract in elderly hypertensive demographic remains unclear and warrants further exploration to aid in strategizing early intervention programs.
Methods
Based on China’s National Basic Public Health Service Project (NBPHSP), a cross-sectional study was conducted in Jia County, Henan Province, China. A total of 17473 cases aged 65 years and over with hypertension were included in this study. Sleep duration was obtained through questionnaires and information on cataracts was derived from NBPHSP. Three logistic regression models were used to assess the association between sleep duration and cataract. Subgroup analysis and interaction tests were performed to address heterogeneity.
Results
The average self-reported sleep duration was (6.77 ± 1.80) hours, and the prevalence of cataracts was 11.9%. In the adjusted logistic regression model, elderly hypertensive patients with sleep duration < 6 hours had a higher risk of cataract compared to those with sleep duration between 7–8 hours (OR: 1.39, 95%CI: 1.21–1.59). However, non-significant association was found between long sleep duration and cataract. The findings from subgroup analysis indicated no significant interaction effect.
Conclusions
In rural China, elderly hypertensive patients with a sleep duration of less than 6 hours are at a significantly higher risk of developing cataracts. This finding underscores the importance of monitoring sleep patterns in this population. Promoting adequate sleep duration may be a key strategy in reducing cataract prevalence and improving the overall quality of life for elderly patients with hypertension.
Evaluating change in self-reported function and pain in clients with musculoskeletal concerns attending a physiotherapy student-led service
Authors: Prudden Chloe , Jones Taryn , Baque Emmah , Collings Tyler , Edwards Sandy , Shephard Renae , Hams Andrea 
Published: October 2024

Abstract

Musculoskeletal conditions are a major contributor to global health burden, often resulting in impaired function and persistent pain. Physiotherapy student-led services provide a model for addressing community healthcare needs while offering authentic clinical training experiences. Despite emerging research on stakeholder perceptions and satisfaction with student-led services, their impact on clinical outcomes remains unclear. This study aimed to evaluate changes in self-reported function and pain and describe demographics including presenting musculoskeletal conditions among clients attending single-site physiotherapy student-led service. Ninety-nine participants (Mdn age = 29 years, IQR = 22–40) were recruited from a South-East Queensland student-led service. Demographic and presenting condition data were collected at the initial appointment, while self-reported outcome measures of function (Patient Specific Functional Scale: PSFS) and pain (Numeric Pain Rating Scale: NPRS) were recorded on a 0–10 scale at all appointments. Results showed a significant positive correlation between appointment frequency and PSFS scores, with each appointment associated with a 0.766 unit increase in PSFS scores (95% CI [0.434, 1.10], t (390) = 4.53, p < .001). There was a significant negative correlation between appointment frequency and NPRS scores, with each appointment associated with a 0.58 unit decrease in NPRS scores (95% CI [-0.77, -0.39], t (141) = -5.99, p < .001). This study adds to growing evidence supporting student-led services as effective settings to improve self-reported function and reduce pain among clients with diverse musculoskeletal conditions. Further multi-site longitudinal research on student-led services is recommended.
Analysis of Dysmenorrhea-Related Factors in Adenomyosis and Development of a Risk Prediction Model
Authors: Fu Yudan , Wang Xin , Yang Xinchun , zhao Ruihua 
Published: October 2024

Abstract

Objective
To explore factors related to dysmenorrhea in adenomyosis and construct a risk prediction model.
Methods
A cross-sectional survey involving 1636 adenomyosis patients from 37 hospitals nationwide (November 2019 - February 2022) was conducted. Data on demographics, disease history, menstrual and reproductive history, and treatment history was collect.Patients were categorized into dysmenorrhea and non-dysmenorrhea groups. Multivariate logistic regression analyzed factors influencing dysmenorrhea, and a risk prediction model was created using a nomogram. The model's performance was evaluated through ROC curve analysis, C-index, Hosmer-Lemeshow test, and bootstrap method The nomogram function was used to establish a nomogram model. The model was evaluated using the area under the ROC curve (AUC), C-index, Hosmer-Lemeshow goodness-of-fit test, and bootstrap method. Patients were scored based on the nomogram, and high-risk groups were delineated.
Results
Dysmenorrhea was present in 61.31% (1003/1636) of the patients. Univariate analysis showed significant differences (P < 0.05) between groups in age at onset, course of disease, oligomenorrhea, menorrhagia, number of deliveries, pelvic inflammatory disease, family history of adenomyosis, exercise, and excessive menstrual fatigue. Significant factors included menorrhagia, multiple deliveries, pelvic inflammatory disease, and family history of adenomyosis as risk factors. Older age at onset, oligomenorrhea, and exercise were identified as protective factors. The model's accuracy, discrimination, and reliability were acceptable, and a risk score > 88.5 points indicated a high-risk group.
Conclusion
Dysmenorrhea is prevalent among adenomyosis patients. Identifying and mitigating risk factors, while leveraging protective factors, can aid in prevention and management. The developed model effectively predicts dysmenorrhea risk, facilitating early intervention and treatment.
Tackling intrinsic instability of mRNA vaccine by a rapid onsite microfluidic assembly (ROMA) technology
Authors: Xu Jiang , Zheng Zhouyi , Jiang Yue , Wang Qiuhe , Dai Zhiqi , Yuan Ruyue , Yu Zhibin , Gong Yue , Hou Fujun , Ding Jun , Zhang Wen , Gu Zhen , Jia William 
Published: October 2024

Abstract

Despite achieving unprecedented success, current mRNA vaccines face significant challenges, including thermo-instability, degradation, and infrastructure-dependence, making customizable supply a distant goal. Here, we describe a Rapid Onsite Microfluidic Assembly (ROMA) technology capable of generating ready-to-inject mRNA vaccines with a real-time quality inspection as a solution. Diverging from traditional manufacturing mechanism of directly assembling mRNA and lipids into mRNA-LNPs, ROMA technology utilizes mRNA and pre-made empty LNPs to form mRNA-LNPs that exhibit equivalent physiochemical parameters and in vivo expressions compared to conventional ones. Our ROMA prototype offers personalized options for mRNA vaccines, including lipid nanoparticle (LNP) sizes, compositions, mRNA types, and dosages tailored to individual needs, at a throughput of 200 doses/hour (∼100 µg mRNA/dose) with scalable potential. Crucially, ROMA mRNA vaccine, immediately deployable without the need for storage, fundamentally avoids the intrinsic thermal instability and degradation risks associated with conventional ones. This transformative ROMA technology offers unparalleled user-end convenience, unlocking the translational potential for personalized mRNA vaccines and treatments, thereby significantly expanding the scope of mRNA-based therapeutics.
Ascitic Shear Stress Activates GPCRs and Downregulates Mucin 15 to Promote Ovarian Cancer Malignancy
Authors: Horst Eric N. , Cotter Liam R. , Bonini Mia , Novak Caymen M. , Treacher Nina M. , Zhang Yeye , Jackson Zoe F. , Narayanan Ishwarya V. , Fischer Zachary S. , Sunshine Alec R. , Lin Zequan , Tran Linh A. , Ljungman Mats , Maturen Katherine E. , DiFeo Analisa , Nordsletten David A. , Mehta Geeta 
Published: October 2024

Abstract

The accumulation of ascites in patients with ovarian cancer increases their risk of transcoelomic metastasis. Although common routes of peritoneal dissemination are known to follow distinct paths of circulating ascites, the mechanisms that initiate these currents and subsequent fluid shear stresses are not well understood. Here we developed a patient-based, boundary driven computational fluid dynamics model to predict an upper range of fluid shear stress generated by the accumulation of ascites. We show that ovarian cancer cells exposed to ascitic shear stresses display heightened G protein-coupled receptor mechanosignaling and the induction of an epithelial to mesenchymal-like transition through p38α mitogen-activated protein kinase and mucin 15 modulation. An emergent immunomodulatory secretome and endoplasmic reticulum stress activation is also present in shear stimulated cancer cells, positioning elevated shear stress as a protumoural signal. Together, these findings suggest maintenance strategies for overcoming mechanotransduction mediated metastasis within the peritoneal cavity.
Cell Populations in Human Breast Cancers are Molecularly and Biologically Distinct with Age
Authors: McAllister Sandra , Parsons Adrienne , Colon Esther Sauras , Spasic Milos , Kurt Busem Binboga , Swarbrick Alexander , Freedman Rachel , Mittendorf Elizabeth , Galen Peter van 
Published: October 2024

Abstract

Aging is associated with increased breast cancer risk and outcomes are worse for the oldest and youngest patients, regardless of subtype. It is not known how cells in the breast tumor microenvironment are impacted by age and how they might contribute to age-related disease pathology. Here, we discover age-associated differences in cell states and interactions in human estrogen receptor-positive (ER+) and triple-negative breast cancers (TNBC) using new computational analyses of existing single-cell gene expression data. Age-specific program enrichment (ASPEN) analysis reveals age-related changes, including increased tumor cell epithelial-mesenchymal transition, cancer-associated fibroblast inflammatory responses, and T cell stress responses and apoptosis in TNBC. ER+ breast cancer is dominated by increased cancer cell estrogen receptor 1 (ESR1) and luminal cell activity, reduced immune cell metabolism, and decreased vascular and extracellular matrix (ECM) remodeling with age. Cell interactome analysis reveals candidate signaling pathways that drive many of these cell states. This work lays a foundation for discovery of age-adapted therapeutic interventions for breast cancer.
Word-In-Noise Perception Test in Adults
Authors: Zafarghandi Mohammad-Ebrahim Mahdavi- , Eghbalian Fatemeh , Najafi-Vosough Roya , Emami Seyed-Faranak 
Published: October 2024

Abstract

Background
The recently designed Word-in-noise Perception (WINP) test is a new technique for examining lexical-semantic scores by homotonic-monosyllabic words (HMWs) and white noise, which assesses auditory brain function in speech consonant detection. It is necessary to have a test that creates the best competitive conditions for evaluating meaning perception. Therefore, this study aimed to design a WINP test for adults using HMWs and spectrum speech noise (SSN).
Methodology:
This study was a test-development type that was conducted in a cross-sectional-comparative way, it included 110 young Persian speakers (61 men and 49 women) with mean age of 20 (0.56) years. The evaluations included checking the state of general health, sleep and mental states, basic audiological evaluations, dichotic digit test and WINP test using HMWs and SNN. HMWs consisted of 6 lists of 25 words with a vowel/consonant/vowel pattern, the words in each list have the same vowel. The content validity of HMWs was confirmed by 20 Persian language experts and calculated using content and index validity ratios. Its reliability was measured based on repeatability in test times, intraclass correlation coefficient and the comparison of HMWs scores in two repetitions. To calculate the norm values, the number of correct responses in the mean measurements was multiplied by 4 and expressed as a percentage. Mann-Whitney tests were used to compare the scores of the groups.
Results
The validity and reliability of WINP test using HMWs and SSN were determined. Mean CVR and mean CVI of the HMWs were equal to 0.99 and to 0.95 respectively. ICC in single and mean measurements were also calculated. An ICC close to 1 indicates greater validity of WPS and better consistency of HMWs across lists. The results showed that the obtained scores are stable and without measurement errors. Normal values of WINP test using HMWs and SSN were gotten, which were 54%, 69%, 82%, 90% and 94% at SNRs of -5, 0, + 5, +10, and + 15 dB, respectively. The variable of gender was not an effective factor for creating a difference in the mean scores of WINP test using HMWs and SSN (P = 0.989). There was no significant difference between WINP mean scores in SNR in different noises between the right and left ears. Also, there was significant difference between mean scores of WINP in SNRs from − 5 to + 15 for left and right ears.
Conclusion
Psychometric properties of the WINP test using HMWs and SNN have been confirmed for Persian speaking adults.