bluuebunny/crossref_metadata_2025_split huggingface dataset:
Misato underlies visceral myopathy in Drosophila
Authors: Min Soohong , Yoon Woongchang , Cho Hyunho , Chung Jongkyeong 
Published: December 2017

AbstractGenetic mechanisms for the pathogenesis of visceral myopathy (VM) have been rarely demonstrated. Here we report the visceral role of misato (mst) in Drosophila and its implications for the pathogenesis of VM. Depletion of mst using three independent RNAi lines expressed by a pan-muscular driver elicited characteristic symptoms of VM, such as abnormal dilation of intestinal tracts, reduced gut motility, feeding defects, and decreased life span. By contrast, exaggerated expression of mst reduced intestine diameters, but increased intestinal motilities along with thickened muscle fibers, demonstrating a critical role of mst in the visceral muscle. Mst expression was detected in the adult intestine with its prominent localization to actin filaments and was required for maintenance of intestinal tubulin and actomyosin structures. Consistent with the subcellular localization of Mst, the intestinal defects induced by mst depletion were dramatically rescued by exogenous expression of an actin member. Upon ageing the intestinal defects were deteriorative with marked increase of apoptotic responses in the visceral muscle. Taken together, we propose the impairment of actomyosin structures induced by mst depletion in the visceral muscle as a pathogenic mechanism for VM.
A new and general approach to signal denoising and eye movement classification based on segmented linear regression
Authors: Pekkanen Jami , Lappi Otto 
Published: December 2017

AbstractWe introduce a conceptually novel method for eye-movement signal analysis. The method is general in that it does not place severe restrictions on sampling frequency, measurement noise or subject behavior. Event identification is based on segmentation that simultaneously denoises the signal and determines event boundaries. The full gaze position time-series is segmented into an approximately optimal piecewise linear function in O(n) time. Gaze feature parameters for classification into fixations, saccades, smooth pursuits and post-saccadic oscillations are derived from human labeling in a data-driven manner. The range of oculomotor events identified and the powerful denoising performance make the method useable for both low-noise controlled laboratory settings and high-noise complex field experiments. This is desirable for harmonizing the gaze behavior (in the wild) and oculomotor event identification (in the laboratory) approaches to eye movement behavior. Denoising and classification performance are assessed using multiple datasets. Full open source implementation is included.
Electrical stimulation of macaque lateral prefrontal cortex modulates oculomotor behavior indicative of a disruption of top-down attention
Authors: Schwedhelm Philipp , Baldauf Daniel , Treue Stefan 
Published: December 2017

AbstractThe lateral prefrontal cortex (lPFC) of primates is hypothesized to be heavily involved in decision-making and selective visual attention. Recent neurophysiological evidence suggests that information necessary for an orchestration of those high-level cognitive factors are indeed represented in the lPFC. However, we know little about the specific contribution of sub-networks within lPFC to the deployment of top-down influences that can be measured in extrastriate visual cortex. Here, we systematically applied electrical stimulations to areas 8Av and 45 of two macaque monkeys performing a concurrent goal-directed saccade task. Despite using currents well above saccadic thresholds of the directly adjacent Frontal Eye Fields (FEF), saccades were only rarely evoked by the stimulation. Instead, two types of behavioral effects were observed: Stimulations of caudal sites in 8Av (close to FEF) shortened or prolonged saccadic reaction times, depending on the task-instructed saccade, while rostral stimulations of 8Av/45 seem to affect the relative attentional weighting of saccade targets as well as saccadic reaction times. These results illuminate important differences in the causal involvement of different sub-networks within the lPFC and are most compatible with a stimulation-induced biasing of stimulus processing that accelerates the detection of saccade targets presented ipsilateral to stimulation through a disruption of contralaterally deployed top-down attention.
Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis
Authors: Chen Congcong , Lu Chaosheng , Qian Yan , Li Haiyan , Tan Yi , Cai Lu , Weng Huachun 
Published: December 2017

AbstractKidney biopsy is considered the golden criterion for diagnosing the etiology of kidney disease but accompanied by non-negligible complications. We explored the possibility of using urinary microRNA (miRNA) as a non-invasive biomarker for hypertensive kidney injury. We assessed differential miRNA expressions in the kidneys and urine of hypertensive mice with kidney injury induced by deoxycorticosterone acetate (DOCA)-salt compared to the controls. DOCA-salt treatment significantly increased renal tubular lesions from day 2 and mRNA expression of fibrosis-related genes from day 4 compared to the controls, respectively. Urinary albumin and N-acetyl-beta-D-glucosaminidase was significantly increased on day 8 compared to the controls. Array results showed that 20 out of 585 miRNAs were highly expressed in the kidneys and significantly increased on day 8 compared to the controls, including miR-21, miR-146b, miR-155 and miR-132, which were confirmed by real-time polymerase chain reaction and were significantly higher from day 4. The miR-21/creatinine in the urine from day 4 was significantly higher than that of the controls and was detected earlier than urinary albumin. In conclusion, we have identified urinary miR-21 that correlates with histopathological lesions and functional markers of kidney damage to facilitate a potential noninvasive detection for hypertensive kidney injury.
Expression profiling of the retina of pde6c, a zebrafish model of retinal degeneration
Authors: Zhang Liyun , Zhang Xinlian , Zhang Gaonan , Pang Chi Pui , Leung Yuk Fai , Zhang Mingzhi , Zhong Wenxuan 
Published: December 2017

AbstractRetinal degeneration often affects the whole retina even though the disease-causing gene is specifically expressed in the light-sensitive photoreceptors. The molecular basis of the retinal defect can potentially be determined by gene-expression profiling of the whole retina. In this study, we measured the gene-expression profile of retinas microdissected from a zebrafishpde6cw59(pde6c) mutant. This retinal-degeneration model not only displays cone degeneration caused by a cone-specific mutation, but also other secondary cellular changes starting from 4 days postfertilization (dpf). To capture the underlying molecular changes, we subjectedpde6cand wild-type (WT) retinas at 5 dpf/ 120 h postfertilization (hpf) to RNA sequencing (RNA-Seq) on the Illumina HiSeq 2,000 platform. We also validated the RNA-Seq results by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) of seven phototransduction genes. Our analyses indicate that the RNA-Seq dataset was of high quality, and effectively captured the molecular changes in the wholepde6cretina. This dataset will facilitate the characterization of the molecular defects in thepde6cretina at the initial stage of retinal degeneration.
An RNA-Seq atlas of gene expression in mouse and rat normal tissues
Authors: Söllner Julia F. , Leparc German , Hildebrandt Tobias , Klein Holger , Thomas Leo , Stupka Elia , Simon Eric 
Published: December 2017

AbstractGene functionality is closely connected to its expression specificity across tissues and cell types. RNA-Seq is a powerful quantitative tool to explore genome wide expression. The aim of this study is to provide a comprehensive RNA-Seq dataset across the same 13 tissues for mouse and rat, two of the most relevant species for biomedical research. The dataset provides the transcriptome across tissues from three male C57BL6 mice and three male Han Wistar rats. We also describe our bioinformatics pipeline to process and technically validate the data. Principal component analysis shows that tissue samples from both species cluster similarly. We show by comparative genomics that many genes with high sequence identity with respect to their human orthologues also have a highly correlated tissue distribution profile and are in agreement with manually curated literature data for human. In summary, the present study provides a unique resource for comparative genomics and will facilitate the analysis of tissue specificity and cross-species conservation in higher organisms.
Temporally inter-comparable maps of terrestrial wilderness and the Last of the Wild
Authors: Allan James R. , Venter Oscar , Watson James E.M. 
Published: December 2017

AbstractWilderness areas, defined as areas free of industrial scale activities and other human pressures which result in significant biophysical disturbance, are important for biodiversity conservation and sustaining the key ecological processes underpinning planetary life-support systems. Despite their importance, wilderness areas are being rapidly eroded in extent and fragmented. Here we present the most up-to-date temporally inter-comparable maps of global terrestrial wilderness areas, which are essential for monitoring changes in their extent, and for proactively planning conservation interventions to ensure their preservation. Using maps of human pressure on the natural environment for 1993 and 2009, we identified wilderness as all ‘pressure free’ lands with a contiguous area >10,000 km2. These places are likely operating in a natural state and represent the most intact habitats globally. We then created a regionally representative map of wilderness following the well-established ‘Last of the Wild’ methodology; which identifies the 10% area with the lowest human pressure within each of Earth’s 60 biogeographic realms, and identifies the ten largest contiguous areas, along with all contiguous areas >10,000 km2.
LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
Authors: Li Jie , Yang Chen , Li Yinguang , Chen Aiyue , Li Li , You Zeshan 
Published: December 2017

Objective: Long noncoding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is involved in various kinds of cancer. However, the role of lncGAS5 in the development of ovarian cancer remains unclear. In the present study, we explored the cellular mechanism and clinical value of lncRNA GAS5 in ovarian cancer.
Methods: Quantitative real-time PCR was used to detect mRNA level of lncRNA GAS5 in 20 ovarian cancer tissues. The effect of lncRNA GAS5 on cell proliferation was performed using CCK-8 assay. Cell apoptosis was evaluated by flow cytometry. Western blotting was used to detect the protein level of lncRNA GAS5 potential target. Standard sandwich ELISA was used to quantify the level of inflammatory cytokines. The cells with stable expression of lncRNA GAS5 were injected into nude mice to study the effect of lncRNA GAS5 on tumorigenesis in vivo.
Results: The expression of lncRNA GAS5 was significantly decreased in ovarian cancer tissues. Decrease in lncRNA GAS5 expression resulted in increased cell proliferation and colony formation and reduced ovarian cancer cell apoptosis. In contrast, exogenous overexpression of lncRNA GAS5 in ovarian cancer cells inhibited proliferation, colony formation, and apoptosis in ovarian cancer cells. In addition, the role of lncRNA GAS5 in ovarian cancer was associated with inflammasome formation and pyroptosis.
Conclusion: These results suggested that lncRNA GAS5 acts as tumor suppressor and could be used as a potential treatment target for diagnosis and therapy of ovarian cancer.
Sex-dependent differences in inflammatory responses during liver regeneration in a murine model of acute liver injury
Authors: Bizzaro Debora , Crescenzi Marika , Di Liddo Rosa , Arcidiacono Diletta , Cappon Andrea , Bertalot Thomas , Amodio Vincenzo , Tasso Alessia , Stefani Annalisa , Bertazzo Valentina , Germani Giacomo , Frasson Chiara , Basso Giuseppe , Parnigotto Pierpaolo , Alison Malcolm Ronald , Burra Patrizia , Conconi Maria Teresa , Russo Francesco Paolo 
Published: December 2017

A sexual dimorphism in liver inflammation and repair was previously demonstrated. Its cellular dissection in the course of acute liver injury (ALI) was explored. BALB/c mice were treated with carbon tetrachloride (CCl4) by intraperitoneal injection and killed after 3, 5, and 8 days. Histological and hepatic cell population analyses were performed. The correlation between androgen receptor (AR) expression and liver recruited inflammatory cells was investigated by treatment with the AR antagonist flutamide. Additionally, patients with a diagnosis of drug induced liver injury (DILI) were included in the study, with a particular focus on gender dimorphism in circulating monocytes. A delayed resolution of necrotic damage and a higher expression of proinflammatory cytokines were apparent in male mice along with a slower recruitment of inflammatory monocytes. F4/80+CD11b+ macrophages and CD11bhighGr-1high monocytes expressed AR and were recruited later in male compared with female livers after CCl4 treatment. Moreover, CD11bhighAR+Gr-1high recruitment was negatively modulated by flutamide in males. Analysis of DILI patients showed overall a significant reduction in circulating mature monocytes compared with healthy subjects. More interestingly, male patients had higher numbers of immature monocytes compared with female patients.
A stronger cytotoxic tissue response was correlated with an impaired recruitment of CD11bhighAR+Gr-1high cells and F4/80+CD11b+ macrophages in the early inflammatory phase under AR signaling. During DILI, a dimorphic immune response was apparent, characterized by a massive recruitment of monocytes to the liver both in males and females, but only in males was this recruitment sustained by a turnover of immature monocytes.
Circularly polarised MIMO ground radiation antennas for wearable devices
Authors: Qu Longyue , Piao Haiyan , Qu Yunhao , Kim Hyung‐Hoon , Kim Hyeongdong 
Published: December 2017

Two ground radiation antennas with polarisation diversity performance were proposed for MIMO WLAN applications in wearable devices. The antennas can simultaneously excite the vertical mode and horizontal mode of the ground plane and the phase difference between the two modes can be controlled by utilising an inductor‐loaded metal strip in the ground plane, generating circular polarisation. A 3:1 VSWR bandwidth of 140 MHz with high isolation (above 17 dB) and a 3 dB axial ratio bandwidth of 100 MHz was obtained. The opposite rotations generated by the two antennas resulted in good diversity performance, which was verified by the measured envelope correlation coefficient.
Research on the ranging statistical distribution of laser radar with a constant fraction discriminator
Authors: Jiancheng Lai , Chunyong Wang , Wei Yan , Zhenhua Li 
Published: December 2017

A theoretical framework is presented to describe the statistical distribution of ranging data obtained by laser radar with a constant fraction discriminator. The solution's correctness has been verified by Monte‐Carlo simulation and fixed‐target‐ranging experiments. Based on the numerical calculations, the influences of delay and attenuation on the distribution of ranging data were discussed comprehensively, and found that there exist optimal delay and attenuation to make laser radar has best precision, the optimal attenuation k  = 0.7 and delay τ  = 2 ns for the calculation condition. At the optimal attenuation and delay, the peak position of ranging data distribution is free of the signal's amplitude, and the ranging data distribution is very close to the normal distribution with R2 0.99991.
Comparative study of SPEX and planetary milling methods for the fabrication of complex metallic alloy nanoparticles
Authors: Zolriasatein Ashkan , Shokuhfar Ali , Safari Fardin , Abdi Nazanin 
Published: December 2017

The objective of the present work is the comparison of two common milling methods for the production of complex metallic alloy nanoparticles. γ‐Al12Mg17 nanoparticles were fabricated using both a planetary ball mill and a SPEX mill. The produced nanoparticles have been compared on the basis of the phase and microstructural characterisations by means of X‐ray diffraction (XRD) and field emission scanning electron microscopy (FESEM), respectively. The crystallite size, lattice strain, and lattice parameter have been calculated from the XRD data followed by an investigation on the powder morphology and particle size by FESEM. It has been indicated that the higher milling speed in the SPEX milling method leads to a higher milling energy and consequently lower particle size. For the same milling time of 2 h, the particle size decreases down to nano‐scale using a SPEX ball mill whereas the planetary ball mill products contained a varied size in the range of micron and submicron regimes. Furthermore, the results showed approximate crystalline sizes of 12 and 85 nm for the Al12Mg17 in SPEX and planetary ball mills after 2 h of milling time, respectively.
MUSE observations of the counter-rotating nuclear ring in NGC 7742
Authors: Martinsson Thomas P. K. , Sarzi Marc , Knapen Johan H. , Coccato Lodovico , Falcón-Barroso Jesús , Elmegreen Bruce G. , de Zeeuw Tim 
Published: December 2017

Aims. We present results from MUSE observations of the nearly face-on disk galaxy NGC 7742. This galaxy hosts a spectacular nuclear ring of enhanced star formation, which is unusual in that it is hosted by a non-barred galaxy, and because this star formation is most likely fuelled by externally accreted gas that counter-rotates with respect to its main stellar body.
Methods. We used the MUSE data to derive the star-formation history (SFH) and accurately measure the stellar and ionized-gas kinematics of NGC 7742 in its nuclear, bulge, ring, and disk regions.
Results. We have mapped the previously known gas counter-rotation well outside the ring region and deduce the presence of a slightly warped inner disk, which is inclined at approximately 6° compared to the outer disk. The gas-disk inclination is well constrained from the kinematics; the derived inclination 13.7° ± 0.4° agrees well with that derived from photometry and from what one expects using the inverse Tully-Fisher relation. We find a prolonged SFH in the ring with stellar populations as old as 2–3 Gyr and an indication that the star formation triggered by the minor merger event was delayed in the disk compared to the ring. There are two separate stellar components: an old population that counter-rotates with the gas, and a young one, concentrated to the ring, that co-rotates with the gas. We recover the kinematics of the old stars from a two-component fit, and show that combining the old and young stellar populations results in the erroneous average velocity of nearly zero found from a one-component fit.
Conclusions. The spatial resolution and field of view of MUSE allow us to establish the kinematics and SFH of the nuclear ring in NGC 7742. We show further evidence that this ring has its origin in a minor merger event, possibly 2–3 Gyr ago.
Horizontal flow below solar filaments
Authors: Ambrož P. , Pötzi W. 
Published: December 2017

Context. Observations of the internal fine structures of solar filaments indicate that the threads of filaments follow magnetic field lines. The magnetic field inside the filament has a strong axial component. Some models of magnetic fields suggest that the field structure in filaments could be caused by the horizontal plasma velocity field near both sides below the filament, where observable shearing effects from the axial component are expected.
Aims. The horizontal velocity field in the vicinity of polarity inversion lines is measured in order to determine, if it exhibits a systematic movement that induces shear along the filament axis and convergence perpendicular to the axis.
Methods. The horizontal velocity was obtained from the displacement of supergranules, which were derived from Doppler measurements in the solar photosphere. Dopplergrams corrected for rigid rotation and p-mode oscillations were further analyzed by local correlation tracking.
Results. Vector fields of the horizontal velocities were measured in 16 areas during 8 time intervals in the years 2000–2002 on both solar hemispheres, each for a few consecutive days. For 64 selected filaments the nearby horizontal velocity vectors were split up into a component along the filament axis and a perpendicular component.
Conclusions. Differences between the axial velocities on both sides of the filaments were calculated. In almost all cases the velocity gradient corresponds to the inclination of the threads observed in Hα images. The average transverse velocity does not show any preferred tendency towards a divergence or convergence to the filament axis. Testing the horizontal velocity for the creation of the differential rotation profile in the photosphere reveals a strong dependence of the averaging process on the scale of our velocities.
Superhumps linked to X-ray emission
Authors: Neustroev V. V. , Page K. L. , Kuulkers E. , Osborne J. P. , Beardmore A. P. , Knigge C. , Marsh T. , Suleimanov V. F. , Zharikov S. V. 
Published: December 2017

Context. We present more than 4 years of Swift X-ray observations of the 2013 superoutburst, subsequent decline and quiescence of the WZ Sge-type dwarf nova SSS J122221.7−311525 (SSS J122222) from 6 days after discovery.
Aims. Only a handful of WZ Sge-type dwarf novae have been observed in X-rays, and until recently GW Lib was the only binary of this type with complete coverage of an X-ray light curve throughout a superoutburst. We collected extensive X-ray data of a second such system to understand the extent to which the unexpected properties of GW Lib are common to the WZ Sge class.
Methods. We collected 60 Swift-XRT observations of SSS J122222 between 2013 January 6 and 2013 July 1. Four follow-up observations were performed in 2014, 2015, 2016 and 2017. The total exposure time of our observations is 86.6 ks. We analysed the X-ray light curve and compared it with the behaviour of superhumps which were detected in the optical light curve. We also performed spectral analysis of the data. The results were compared with the properties of GW Lib, for which new X-ray observations were also obtained.
Results. SSS J122222 was variable and around five times brighter in 0.3–10 keV X-rays during the superoutburst than in quiescence, mainly because of a significant strengthening of a high-energy component of the X-ray spectrum. The post-outburst decline of the X-ray flux lasted at least 500 d. The data show no evidence of the expected optically thick boundary layer in the system during the outburst. SSS J122222 also exhibited a sudden X-ray flux change in the middle of the superoutburst, which occurred exactly at the time of the superhump stage transition. A similar X-ray behaviour was also detected in GW Lib.
Conclusions. We show that the X-ray flux exhibits changes at the times of changes in the superhump behaviour of both SSS J122222 and GW Lib. This result demonstrates a relationship between the outer disc and the white dwarf boundary layer for the first time, and suggests that models for accretion discs in high mass ratio accreting binaries are currently incomplete. The very long decline to X-ray quiescence is also in strong contrast to the expectation of low viscosity in the disc after outburst.