bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green‐Light‐Induced Inactivation of Receptor Signaling Using Cobalamin‐Binding Domains
Authors: Kainrath Stephanie , Stadler Manuela , Reichhart Eva , Distel Martin , Janovjak Harald 
Published: March 2017

AbstractOptogenetics and photopharmacology provide spatiotemporally precise control over protein interactions and protein function in cells and animals. Optogenetic methods that are sensitive to green light and can be used to break protein complexes are not broadly available but would enable multichromatic experiments with previously inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12) binding domains of bacterial CarH transcription factors for green‐light‐induced receptor dissociation. In cultured cells, we observed oligomerization‐induced cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin‐binding domains in the dark that was rapidly eliminated upon illumination. In zebrafish embryos expressing fusion receptors, green light endowed control over aberrant fibroblast growth factor signaling during development. Green‐light‐induced domain dissociation and light‐inactivated receptors will critically expand the optogenetic toolbox for control of biological processes.
Activation of SF6 at Platinum Complexes: Formation of SF3 Derivatives and Their Application in Deoxyfluorination Reactions
Authors: Berg Claudia , Braun Thomas , Ahrens Mike , Wittwer Philipp , Herrmann Roy 
Published: March 2017

AbstractThe activation of SF6 at [Pt(PR3)2] R=Cy, iPr complexes in the presence of PR3 led selectively and in an unprecedented reaction route to the generation of the SF3 complexes trans‐[Pt(F)(SF3)(PR3)2]. These can also be synthesized from SF4 and the SF2 derivative trans‐[Pt(F)(SF2)(PCy3)2][BF4] was characterized by X‐ray crystallography. trans‐[Pt(F)(SF3)(PR3)2] complexes are useful tools for deoxyfluorination reactions and novel fluorido complexes bearing a SOF ligand are formed. Based on these studies a process for the deoxyfluorination of ketones was developed with SF6 as fluorinating agent.
Diversity in Gold Finger Structure Elucidated by Traveling‐Wave Ion Mobility Mass Spectrometry
Authors: Du Zhifeng , de Paiva Raphael E. F. , Nelson Kristina , Farrell Nicholas P. 
Published: March 2017

AbstractTraveling wave ion mobility (TWIM) mass spectrometry (MS) is a powerful method for the structural and conformational analysis of proteins and peptides, enabling the differentiation of isomeric peptides (or proteins) that have the same sequence but are modified at different residues. In this study, the TWIM‐MS technique was used to separate isomeric AuI metallopeptide ions that were formed by ZnII displacement from the parent zinc fingers (ZFs). The synthetic gold finger peptides were derived from the C‐terminus of the HIV nucleocapsid p7 protein (NCp7‐F2) and finger 3 of the Sp1 transcription factor (Sp1‐F3). TWIM‐MS enabled the acquisition of distinct product ion spectra for each isomer, clearly indicating the binding sites for the major conformers in the presence of multiple coordination possibilities. Collision cross‐section measurements showed that the aurated peptide has a slightly more compact structure than the parent zinc compound NCp7‐F2, which showed only one conformation.
Zinc Regulates Chemical‐Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry
Authors: Ren Lin , Pour Masoumeh Dowlatshahi , Majdi Soodabeh , Li Xianchan , Malmberg Per , Ewing Andrew G. 
Published: March 2017

AbstractWe applied electrochemical techniques with nano‐tip electrodes to show that micromolar concentrations of zinc not only trigger changes in the dynamics of exocytosis, but also vesicle content in a model cell line. The vesicle catecholamine content in PC12 cells is significantly decreased after 100 μm zinc treatment, but, catecholamine release during exocytosis remains nearly the same. This contrasts with the number of molecules stored in the exocytosis vesicles, which decreases, and we find that the amount of catecholamine released from zinc‐treated cells reaches nearly 100 % content expelled. Further investigation shows that zinc slows down exocytotic release. Our results provide the missing link between zinc and the regulation of neurotransmitter release processes, which might be important in memory formation and storage.
Poly(hydroxybutyrate‐co‐hydroxyvalerate) and bovine serum albumin blend prepared by electrospinning
Authors: Pavlova Elizaveta R. , Bagrov Dmitry V. , Kopitsyna Maria N. , Shchelokov Dmitry A. , Bonartsev Anton P. , Zharkova Irina I. , Mahina Tatiana K. , Myshkina Vera L. , Bonartseva Galina A. , Shaitan Konstantin V. , Klinov Dmitry V. 
Published: March 2017

ABSTRACTElectrospinning is a method for the preparation of nanosized polymer fibers. Here, electrospinning is used to prepare a blend of a polyester, poly(hydroxybutyrate‐co‐hydroxyvalerate) (PHBV), and a globular protein, bovine serum albumin (BSA). The electrospun blend film is compared with a solution‐cast blend film and with single‐component electrospun films made of PHBV and BSA. In the electrospun blend films, BSA manifests itself as flat ribbons and a fine network formed from fibers less than 50 nm in diameter. The dissolution rate of BSA from the electrospun blended film is lower than from the solution‐cast one. The films are characterized using scanning electron microscopy, differential scanning calorimetry, and contact‐angle measurements. The obtained PHBV+BSA blend films have several emergent properties: a slow BSA dissolution rate, a fine BSA network, and unusual thermal behavior. Thus, the PHBV+BSA blend films introduce a new class of polymer–protein blends. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45090.
Free mycophenolic acid determination in human plasma ultrafiltrate by a validated liquid chromatography–tandem mass spectrometry method
Authors: Łuszczyńska Paulina , Pawiński Tomasz , Kunicki Paweł K. , Sikorska Katarzyna , Marszałek Ryszard 
Published: March 2017

AbstractThe aim of this study was to develop and validate fully the liquid chromatography–tandem mass spectrometry method for free mycophenolic acid (MPA) concentration measurements in plasma ultrafiltrate that will be reliable and simple in preparation with deuterated MPA (MPA‐d3) chosen as an internal standard. The chromatographic separation was made with Zorbax Eclipse XDB‐C18 column (4.6 × 150 mm) using a gradient of two solutions as a mobile phase: (A) water and (B) methanol, each containing 0.1% formic acid and 2.5 mm ammonium acetate. Satisfactory repeatability of retention times was achieved with average values of 7.54 ± 0.20 min and 7.50 ± 0.19 min for MPA and MPA‐d3, respectively. The method was selective, with no carry‐over or matrix effect observed. The analytical range was proven for MPA ultrafiltrate concentrations of 1–500 ng/mL. The accuracy and precision fell within the acceptance criteria for intraday (accuracy: 100.63–110.46%, imprecision: 6.23–7.76%), as well as interday assay (accuracy: 98.81–110.63%; imprecision: 5.36–10.22%). The method was used for free MPA determination in plasma samples from patients treated with mycophenolate mofetil. To the best of our knowledge this is the first liquid chromatography–tandem mass spectrometry method for free MPA monitoring using MPA‐d3 that allows to measure plasma ultrafiltrate concentrations as low as 1 ng/mL.
Does Innovation Drive Environmental Disclosure? A New Insight into Sustainable Development
Authors: Radu Camélia , Francoeur Claude 
Published: March 2017

AbstractSustainable development is a hot topic in business and the media, and there is a growing demand for reliable environmental disclosure from a wide range of stakeholders. Ethical performance, including social and environmental performance, is actively scrutinized. A firm's stakeholders expect reliable disclosure to correctly assess its performance. Research on the link between environmental disclosure and environmental performance shows mixed results. Both a positive and a negative association have been found. This study reexamines this association by considering environmental innovation as a key determinant of environmental disclosure. We find that environmental performance and environmental innovation jointly determine environmental disclosure. At low levels of environmental performance, innovative firms tend to disclose more than their non‐innovative counterparts to inform stakeholders about their innovation and strategy to obtain an improved environmental performance. This disclosure gap tends to diminish as innovative firms become better environmental performers. The higher levels of environmental disclosure are closely associated with firms' environmental performance for both groups. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
Do men and women use different tactics to cope with the embarrassment of buying condoms?
Authors: Arndt Aaron D. , Ekebas‐Turedi Ceren 
Published: March 2017

AbstractIt has been proposed that people cope with the embarrassment of acquiring condoms using a variety of tactics, such as buying from a sales clerk of the same gender or buying additional products. However, the actual use and efficacy of these coping mechanisms have not been rigorously evaluated. This research investigates three specific questions related to coping with embarrassment: (R1) Do people prefer cashiers of the same gender when buying condoms because it helps them cope with embarrassment or because of the similarity‐attraction effect? (R2) When multiple coping mechanisms are available, do people use more than one at a time? And (R3) Do men and women prefer different coping tactics? Using an experimental design, Study 1 investigates R1 and finds support for both explanations. Studies 2 and 3 use field and lab experiments, respectively, to investigate R2 and R3. The findings indicate that people only use one coping tactic at a time and that men and women have a different preference for coping tactics. Women prefer coping tactics that mask condom acquisition, while men prefer social–emotional support tactics. Furthermore, discrepancies between the field experiment and lab experiments indicate that people underestimate the influence of embarrassment on their actual behavior.
Plaque erosion delays vascular healing after drug eluting stent implantation in patients with acute coronary syndrome
Authors: Hu Sining , Wang Chao , Zhe Chunyang , Zhu Yinchun , Yonetsu Taishi , Jia Haibo , Hou Jingbo , Zhang Shaosong , Jang Ik‐Kyung , Yu Bo 
Published: March 2017

ObjectivesTo compare vascular healing after drug‐eluting stent (DES) implantation between plaque rupture (PR) and plaque erosion (PE).BackgroundVascular response after stent implantation in patients with PR has been extensively studied. Little is known about vascular healing after stent implantation in PE.MethodsSixty‐five ACS patients who received optical coherence tomography (OCT) imaging of the culprit lesions both before and after stent implantation at baseline as well as at 6 months were included in this study. Patients were divided into two groups: PR (n = 19) and PE (n = 24). Prestent thrombus burden and poststent intrastent structure (ISS) volume were analyzed during the index procedure. The ratio of uncovered to total stent struts per cross‐section score (RUTTS) and neointimal thickness and area were measured at follow‐up.ResultsOCT imaging showed that compared with PR, PE showed a significantly lower prestent thrombus score (34.2 ± 19.2 vs. 68.6 ± 44.2, P = 0.009) at baseline and a smaller poststent ISS volume (0.7 ± 0.9 mm3 vs. 2.1 ± 1.9 mm3, P = 0.019). At the 6‐month follow‐up, PE showed a higher incidence of RUTTS >0.3 (12.2 ± 14.4 vs. 2.0 ± 4.5%, P = 0.003), thinner neointimal thickness (0.05 ± 0.02 mm vs. 0.12 ± 0.08 mm, P = 0.002), and smaller neointimal area (0.5 ± 0.2 vs. 1.2 ± 0.9 mm2, P = 0.004) compared with PR. In a multivariate logistic model, PE was identified as an independent predictor for RUTTS >0.3.ConclusionsPE was associated with less favorable healing following DES implantation when compared to PR at 6 months, indicating longer dual‐antiplatelet therapy may be necessary for patients with PE. © 2017 Wiley Periodicals, Inc.
Cooperative Magnetism in Crystalline N‐Aryl‐Substituted Verdazyl Radicals: First‐Principles Predictions and Experimental Results
Authors: Eusterwiemann Steffen , Dresselhaus Thomas , Doerenkamp Carsten , Janka Oliver , Niehaus Oliver , Massolle Anja , Daniliuc Constantin G. , Eckert Hellmut , Pöttgen Rainer , Neugebauer Johannes , Studer Armido 
Published: March 2017

AbstractWe report on a series of eight diaryl‐6‐oxo‐verdazyl radicals containing a tert‐butyl group at the C(3) position with regard to their crystal structure and magnetic properties by means of magnetic susceptibility measurements in combination with quantum chemical calculations using a first‐principles bottom‐up approach. The latter method allows for a qualitative prediction and detailed analysis of the correlation between the solid‐state architecture and magnetic properties. Although the perturbation in the molecular structure by varying the substituent on the N‐aryl ring may appear small, the effects upon the structural parameters controlling intermolecular magnetic coupling interactions are strong, resulting in a wide spectrum of cooperative magnetic behavior. The non‐substituted 1,5‐diphenyl‐tert‐butyl‐6‐oxo‐verdazyl radical features a ferromagnetic one‐dimensional spin ladder type magnetic network—an extremely rarely observed phenomenon for verdazyl radicals. By varying substituents at the phenyl group, different non‐isostructural compounds were obtained with widely different magnetic motifs ranging from linear and zigzag one‐dimensional chains to potentially two‐dimensional networks, from which we predict magnetic susceptibility data that are in qualitative agreement with experiments and reveal a large sensitivity to packing effects of the molecules. The present study advances the fundamental understanding between solid‐state structure and magnetism in organically based radical systems.
Preparation and Characterization of High Content Paraffin Wax Microcapsules and Micro/Nanocapsules with Poly Methyl Methacrylate Shell by Suspension‐Like Polymerization
Authors: Zhang Jiaojiao , Zhao Tianbo , Chai Yuqiao , Wang Lili 
Published: March 2017

AbstractThe suspension‐like polymerization method is used to obtain poly methyl methacrylate (PMMA)/paraffin wax microcapsules and micro/nanocapsules with high core content. Particle size distribution (PSD) analysis indicates that the average particle size of microcapsules is 94 µm, and the size of micro/nanocapsules ranges in 0.1‐19 µm. Differential scanning calorimetry (DSC) and thermalgravimetric analysis (TGA) results show that the fabricated paraffin contents in microcapsules and micro/nanocapsules are as high as 89.5 wt% and 80.2 wt%, respectively with good thermal stability. Thermal cycling tests justify that both the microcapsules and micro/ nanocapsules have good thermal reliability with respect to the changes in their thermal properties after 1000 thermal cycling. This work provides a novel method to prepare microcapsules and micro/nanocapsules with high core content which may benefit further study on thermal energy storage.
Cognitive radio network with coordinated multipoint joint transmission
Authors: Reasat Tahsin , Saha Abir , Uddin Md. Forkan 
Published: March 2017

SummaryCognitive radio (CR) is considered to be a promising technology for future wireless networks to make opportunistic utilization of the unused or underused licensed spectrum. Meanwhile, coordinated multipoint joint transmission (CoMP JT) is another promising technique to improve the performance of cellular networks. In this paper, we propose a CR system with CoMP JT technique. We develop an analytical model of the received signal‐to‐noise ratio at a CR to determine the energy detection threshold and the minimum number of required samples for energy detection–based spectrum sensing in a CR network (CRN) with CoMP JT technique. The performance of energy detection–based spectrum sensing under the developed analytical model is evaluated by simulation and found to be reliable. We formulate an optimization problem for a CRN with CoMP JT technique to configure the channel allocation and user scheduling for maximizing the minimum throughput of the users. The problem is found to be a complex mixed integer linear programming. We solve the problem using an optimization tool for several CRN instances by limiting the number of slots in frames. Further, we propose a heuristic‐based simple channel allocation and user scheduling algorithm to maximize the minimum throughput of the users in CRNs with CoMP JT technique. The proposed algorithm is evaluated via simulation and found to be very efficient.
Evidence of boldenone, nandrolone, 5(10)‐estrene‐3β‐17α‐diol and 4‐estrene‐3,17‐dione as minor metabolites of testosterone in equine
Authors: Wong Jenny K. Y. , Leung David K. K. , Curl Peter , Schiff Peter J. , Lam Kenneth K. H. , Wan Terence S. M. 
Published: March 2017

The detection of boldenone, nandrolone, 5(10)‐estrene‐3β,17α‐diol, and 4‐estrene‐3,17‐dione in a urine sample collected from a gelding having been treated with testosterone (500 mg ‘Testosterone Suspension 100’, single dose, injected intramuscularly) in 2009 led the authors' laboratory to suspect that these ‘testicular’ steroids could be minor metabolites of testosterone in geldings. Administration trials on six castrated horses with Testosterone Suspension 100 confirmed that low levels of boldenone, nandrolone, 5(10)‐estrene‐3β,17α‐diol, and 4‐estrene‐3,17‐dione could indeed be detected and confirmed in the early post‐administration urine samples from all six geldings. Although boldenone has been reported to be present in urine after testosterone administration, there has been no direct evidence reported that boldenone, nandrolone, 5(10)‐estrene‐3β,17α‐diol, and 4‐estrene‐3,17‐dione are metabolites of testosterone in geldings. Subsequent in vitro experiments involving the incubation of testosterone with horse liver microsomes, liver, adipose and muscle tissues, and adrenal cortex homogenates failed to provide evidence that these four substances are minor metabolites of testosterone. An administration trial using ‘Testosterone Suspension 100’ supplemented with 13C–labelled testosterone (500 mg, 1:1 ratio, injected intramuscularly) was performed. The similarities of the excretion curves of 12C–testosterone and 13C–testosterone in urine suggest that there was minimal kinetic isotope effect. 13C–Labelled boldenone, nandrolone and 4‐estrene‐3,17‐dione were detected but not 5(10)‐estrene‐3β,17α‐diol and its 13C–counterpart. This is the first unequivocal evidence of boldenone, nandrolone and 4‐estrene‐3,17‐dione being metabolites of testosterone in geldings. In view of these results, caution should be exercised when interpreting findings of boldenone, nandrolone and/or 4‐estrene‐3,17‐dione together with a relatively high level of testosterone in gelding urine. Copyright © 2017 John Wiley & Sons, Ltd.
Conditions on the Mexican moulting grounds influence feather colour and carotenoids in Bullock's orioles (Icterus bullockii)
Authors: Sparrow Kaitlin L. , Donkor Kingsley K. , Flood Nancy J. , Marra Peter P. , Pillar Andrew G. , Reudink Matthew W. 
Published: March 2017

AbstractCarotenoid‐based plumage coloration plays a critical role for both inter‐ and intrasexual communication. Habitat and diet during molt can have important consequences for the development of the ornamental signals used in these contexts. When molt occurs away from the breeding grounds (e.g., pre‐alternate molt on the wintering grounds, or stopover molt), discerning the influence of habitat and diet can be particularly important, as these effects may result in important carryover effects that influence territory acquisition or mate choice in subsequent seasons. Several species of songbirds in western North America, including the Bullock's oriole (Icterus bullockii), migrate from the breeding grounds to undergo a complete prebasic (post‐breeding) molt at a stopover site in the region affected by the Mexican monsoon climate pattern. This strategy appears to have evolved several times independently in response to the harsh, food‐limited late‐summer conditions in the arid West, which contrast strongly with the high productivity driven by heavy rains that is characteristic of the Mexican monsoon region. Within this region, individuals may be able to optimize plumage coloration by molting in favourable areas characterized by high resource abundance. We used stable isotope analysis (δ13C, δ15N) to ask whether the diet and molt habitat/location of Bullock's orioles influenced their expression of carotenoid‐based plumage coloration as well as plumage carotenoid content and composition. Bullock's orioles with lower feather δ15N values acquired more colorful plumage (orange‐shifted hue) but had feathers with lower total carotenoid concentration, lower zeaxanthin concentration, and marginally lower canthaxanthin and lutein concentration. Examining factors occurring throughout the annual cycle are critical for understanding evolutionary and ecological processes. Here, we demonstrate that conditions experienced during a stopover molt, occurring hundreds to thousands of kilometers from the breeding grounds, influence the production of ornamental plumage coloration, which may carryover to influence inter‐ and intrasexual signaling in subsequent seasons.
Forage plants of an Arctic‐nesting herbivore show larger warming response in breeding than wintering grounds, potentially disrupting migration phenology
Authors: Lameris Thomas K. , Jochems Femke , van der Graaf Alexandra J. , Andersson Mattias , Limpens Juul , Nolet Bart A. 
Published: March 2017

AbstractDuring spring migration, herbivorous waterfowl breeding in the Arctic depend on peaks in the supply of nitrogen‐rich forage plants, following a “green wave” of grass growth along their flyway to fuel migration and reproduction. The effects of climate warming on forage plant growth are expected to be larger at the Arctic breeding grounds than in temperate wintering grounds, potentially disrupting this green wave and causing waterfowl to mistime their arrival on the breeding grounds. We studied the potential effect of climate warming on timing of food peaks along the migratory flyway of the Russian population of barnacle geese using a warming experiment with open‐top chambers. We measured the effect of 1.0–1.7°C experimental warming on forage plant biomass and nitrogen concentration at three sites along the migratory flyway (temperate wintering site, temperate spring stopover site, and Arctic breeding site) during 2 months for two consecutive years. We found that experimental warming increased biomass accumulation and sped up the decline in nitrogen concentration of forage plants at the Arctic breeding site but not at temperate wintering and stop‐over sites. Increasing spring temperatures in the Arctic will thus shorten the food peak of nitrogen‐rich forage at the breeding grounds. Our results further suggest an advance of the local food peak in the Arctic under 1–2°C climate warming, which will likely cause migrating geese to mistime their arrival at the breeding grounds, particularly considering the Arctic warms faster than the temperate regions. The combination of a shorter food peak and mistimed arrival is likely to decrease goose reproductive success under climate warming by reducing growth and survival of goslings after hatching.