bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSLBII-29 Effects of heat stress mitigation methods on growth performance, environmental, and economic outcomes of feedlot cattle raised in a hot climate
Authors: Turcios Sebastian E Mejia , Rotz Alan , McGlone John J , Rivera Carlos , Mitloehner Frank M 
Published: September 2024

Abstract
The increase in average global temperatures presents a challenge for the beef industry, especially in the feedlot sector where heat stress is a major animal welfare and economic concern. To combat this, shade stands out as one of the most practical methods to mitigate heat stress in feedlot cattle. An experiment was conducted as a completely randomized design with Bos indicus bulls (n = 1,560) with initial body weights (BW) of 287 kg. Three shade structure types were used to investigate the effects of different heat stress mitigation methods on cattle growth performance, environmental, and economic outcomes using live animal data, and a partial lifecycle assessment (LCA) using the Integrated Farm System Model (IFSM). The live animal portion of the experiment was done once a year over a 2-yr period with three pen replications per treatment per year (n = 6 per treatment). Four shade structures used were: conventional shade (SC; steel shade 1.8 m2 of shade/animal), double conventional shade (DS; steel shade 3.6 m2 of shade/animal), dome structures without fans (DSA; 8.5 m2/animal with 98% solar radiation blocked), and domes with fans (DCA; three large sized low-speed fans). Each pen held 65 bulls in an area of 570 m2. Live animal data were analyzed as a completely randomized design using the GLM procedure of SAS (version 9.4) with shade type as fixed effect, pen as the experimental unit, and repetition (year) considered a random effect. Cattle housed under DCA had 22 kg and 20 kg heavier final body BW (P &lt; 0.05) compared with those housed under SC and DS, respectively. Final BW of DCA and DSA cattle were similar (P &gt; 0.10). Average daily gain, feed efficiency, and hot carcass weight (HCW) were greater (P &lt; 0.05) for cattle housed under DCA compared with the rest of the shade types. Dry matter intake was not affected (P &gt; 0.10). When treatment results were extrapolated to the annual feedlot turnover of 209,700 animals, cattle in DSA and DCA versus SC and DS had 3 to 8 % reductions in greenhouse gas and ammonia emissions intensities. Compared with SC, DCA increased profitability by $29.66/animal, followed by DSA and DS with profit increases of $5.79 and $8.90/animal, respectively. Overall, the implementation of advanced shade structures improved cattle performance and profitability while reducing the environmental impact of beef production.
PSLBII-4 The effect of standardized ileal digestible sulfur amino acid intake during late gestation on gilt whole-body nitrogen retention and subsequent litter performance
Authors: Alfonso Cristhiam J Munoz , Huber Lee-Anne , Levesque Crystal L 
Published: September 2024

Abstract
Gestating gilts [n = 27; body weight (BW) = 158 ± 5.5 kg] were used to determine the effect of standardized ileal digestible (SID) sulfur amino acid (SAA) intake during late gestation on whole-body nitrogen (N) retention and subsequent litter performance. Gilts were assigned to one of two experimental diets that provided SAA at 60 or 200 % of estimated requirements during late gestation (0.29 and 0.92 % SID SAA, respectively; n = 15 and 12, respectively; Table 1 and 2). Each gilt received 2.53 kg of the assigned diet between gestation d 90 and farrowing; standard lactation and nursery diets were provided to gilts after farrowing and offspring after weaning, respectively. Gilt whole-body N balance was determined between d 107 and 109 of gestation using total urine collection and fecal grab-sampling. After farrowing, litters were standardized to 13 piglets and were not offered creep feed. Whole-body N retention was greater in gilts fed the diet containing 0.92 vs. 0.29 % SID SAA in late gestation (27.2 vs. 19.3 ± 1.8 g/d; P &lt; 0.05; Table 3), but the number of piglets born alive, litter birth weight, subsequent piglet growth rates, and litter size at weaning were not different between treatment groups. The post-weaning growth performance of offspring was also not influenced by maternal dietary treatment in late gestation (Table 4). At farrowing, the post-absorptive plasma concentration of Cys tended to be less (P = 0.072) and taurine was greater (P &lt; 0.01) for gilts fed 0.92 vs. 0.29 % SID SAA in late gestation; however, there were no differences in plasma SAA metabolites concentrations for gilts at weaning or offspring at birth (Table 5. At weaning, offspring from gilts fed 0.92 % SID SAA had greater plasma homocysteine (P &lt; 0.05) and plasma Cys concentrations (tendency; P = 0.072) vs. offspring from gilts fed 0.29 % SID SAA in late gestation. Three weeks after weaning, plasma taurine concentration was less (P &lt; 0.05) for offspring from gilts fed 0.92 vs. 0.29 % SID SAA in late gestation (Table 6). Six weeks after weaning, plasma glutathione concentration tended to be less (P = 0.057) for offspring from gilts fed 0.92 vs 0.29 % SID SAA in late gestation. When SAA were fed below estimated requirements during late gestation, whole-body N retention was reduced, but fetal growth appeared to be maintained, likely at the expense of maternal N retention. Such divergences in dietary SAA supply in late gestation had no effect on subsequent offspring growth performance, but it appeared that offspring SAA metabolism was impacted until at least 6 wk after weaning. The influence of differences in sulfur AA metabolites on offspring performance beyond the nursery phase remains unknown. Therefore, carry-over effects of maternal SAA supply in late gestation should be considered when developing feeding recommendations for gestating gilts.
PSLBII-2 Impact of controlled feeding on enteric methane production and animal performance
Authors: Salisbury Carlee M , Smith Zachary K , Thompson Logan R 
Published: September 2024

Abstract
The impact ruminants have on environmental sustainability has been of growing concern in recent decades. Many cattle in the northern great plains are fed on family-owned farms and feedlots, highlighting the need for producer-friendly mitigation strategies, such as the established, but not widely adopted management strategy of controlled or precision feeding. The objective of this experiment was to determine the impact of precision feeding on enteric methane (CH4) production and subsequent animal performance. Angus-cross steers [n = 48; body weight (BW) = 447 ± 4.4kg] were blocked by BW and assigned to one of three treatment groups. Treatments consisted of a control (CON) where steers were fed ad libitum, Treatment 1 (TRT1) 96 percent of ad libitum and Treatment 2 (TRT2) 92 percent of ad libitum. TRT1 and TRT2 were adjusted once weekly based on the CON steers average intakes from the previous week. BW was measured on d 0, 1, 28, 56, and 84 and dry matter intake (DMI; kg/d) was measured using an Insentec Roughage Intake Control System (Insentec, Markenesse, The Netherlands). Enteric CH4 and carbon dioxide (CO2) production was determined utilizing 2 GreenFeed emission measurement systems (AHCS; C-Lock Inc., Rapid City, SD). Only visits greater than 3 min in duration were used for analysis. Average Daily Gain (ADG) and gain to feed (G:F) did not differ among treatments (P = 0.17, P = 0.80, respectively), but ADG had a tendency to decrease (linear; P = 0.09). DMI was greatest for CON and least for TRT2 (P ≤ 0.01). CH4 (g/d) output differed between treatments (P ≤ 0.007). There was a tendency for a quadratic relationship for derived methane yield (MY; g/kg DMI) among treatments (P = 0.10). Emission intensity (EI; g CH4/kg gain) was not different across treatments (P = 0.40). These results suggest precision feeding may have a positive impact on methane emissions but could negatively impact performance.
PSLBII-18 Transcriptomic comparison of embryonic tissues between high and low fertility sired pregnancies
Authors: Jiménez Odile Polanco , Melo Gabriela , Ortega Sofia , Oliviera Filho Ramiro V , Franco Gessica , Cerri Ronaldo , Pohler Ky G , Moraes Vasconcelos Jose Luiz M 
Published: September 2024

Abstract
Pregnancy loss is considered one of the main causes of reproductive inefficiency in the beef cattle industry. The objective was to determine the differentially expressed genes (DEGs) between the conceptus and uterus of pregnancies produced by sires of known fertility. We hypothesized that the paternal genome would have a significant effect on transcript abundance between these tissues. The sires used have been previously reported to have significantly different levels of field fertility; however, they had passed all normal quality control standards for frozen thawed semen. Bos indicus heifers (n = 45) were subjected to estrus synchronization and embryo transfer with IVP embryos from either a High Fertility (HF) or a Low Fertility sire (LF), pregnancies were confirmed at slaughter. Samples were collected from the trophectoderm (TE) and caruncle (CAR) on d 25 and 36 of gestation for transcriptome analysis. Total RNA was isolated from tissue samples using the RNeasy kit (QIAGEN; Hilden, Germany) per manufacturer’s instructions. Sequencing was conducted using an Illumina platform. Sequences were aligned to the reference genome ARS-UCD1.2. DEGs between sires and tissue were determined using edge-R package from R. False discovery rate was 0.1. On d 25, out of 15,755 genes expressed in the trophectoderm, 19 genes were downregulated, and 9 genes were upregulated in the LF. In the CAR, 9 genes were downregulated and 7 were upregulated. On d 36, in the TE the LF resulted in 53 downregulated genes and 20 upregulated genes. In CAR there were 18 downregulated genes for the LF whereas 22 genes were upregulated. Gene ontology analysis reported DEGs in the LF compared with the HF were associated with immunology and reproduction. Of particular interest BoLA NC-1
PSLBII-19 Effects of high-forage backgrounding duration on dry matter intake during the early finishing phase
Authors: Pucetti Pauliane , De Filho Sebastiao Campos Valadares , da Silva Julia Travassos , Siqueira Isabelle Pinheiro , Pacheco Renata Pereira , Gandra Livia Moreira , Swanson Kendall C 
Published: September 2024

Abstract
The adaptation of animals to high-concentrate diets is crucial for optimizing performance in the finishing phase. However, the transition to concentrate-rich diets can lead to metabolic disorders and fluctuations in feed intake. Typically, the same adaptation protocol is used regardless of the previous feeding history of the animal. However, it is possible that the backgrounding period duration may influence initial feed intake and subsequently affect animal performance in the finishing phase. Thus, the objective of this study was to evaluate the effects of implementing different high-forage backgrounding (HFB) durations on daily dry matter intake (DMI) and DMI variance over a 28-d period following the 14-d step-up adaptation to the finishing diets of Nellore bulls. Young Nellore bulls [n = 36; average initial body weight (BW) = 265.25 ± 5 kg; age = 7 ± 1 mo] were used in a completely randomized design and assigned to one of four treatment groups (n = 9/group): 0 (HFB), 28 (HFB28), 56 (HFB56), or 84 (HFB84) d of HFB before starting the 14-d step-up adaptation period to the finishing phase. The HFB diet consisted of 800 g/kg of sorghum silage and 200 g/kg of concentrate, while the finishing diet contained 200 g/kg of corn silage and 800 g/kg of concentrate. The diets were formulated according to BR-CORTE (2016) to achieve ADG of 0.4 kg/d during the HFB phase and 1.2 kg/d during the finishing phase. All animals were fed for ad libitum intake in an electronic feeder (INTERGADO, Intergado Ltd., Contagem, MG, Brazil), and individual daily feed intake was recorded. Additionally, DMI variance was calculated for each bull from daily DMI data over the 28 d of the finishing phase after the 14-d step-up period. Data were analyzed using the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC) assuming the significance level of 0.05. The Tukey test was employed to compare means. Daily DMI was analyzed as repeated measures. The HFB duration had no influence on average DMI of the first 28 d of the finishing phase (P = 0.10; Averages per treatment (kg/d): HFB0 = 7.34, HFB28 = 6.53, HFB56 = 6.71, HFB84 = 6.02). However, a significant interaction was observed between treatments and day on finishing phase (P &lt; 0.001), indicating that the effects of different HFB duration may vary over time during the finishing phase, influencing the daily DMI. Furthermore, the HFB84 presented a higher DMI variance, while the remaining treatments did not differ significantly among themselves (P &lt; 0.001; Averages per treatment: HFB0 = 0.927, HFB28 = 0.616, HFB56 = 0.980, HFB84 = 3.487). In conclusion, the HFB84 increased the variation in daily DMI after adaptation to the finishing diet, indicating that a longer adaptation period may be needed for animals fed high-forage backgrounding diets for longer durations.
PSVII-30 Winter-feeding high concentrate diets reduces enteric methane emissions pre-calving in beef cows
Authors: Wehrbein Megan A , Moreno Elias Velasquez , Menendez Hector M , Rusche Warren C , Smith Zachary K , Menezes Ana Clara B 
Published: September 2024

Abstract
Objectives were to evaluate the effects of winter-feeding forage (2.02 Mcal/kg dietary ME) vs. concentrate (2.84 Mcal/kg of dietary ME) diets pre- and post- calving on enteric methane emissions, dry matter intake (DMI), and performance of beef cows. Forty-six pregnant (210 ± 10 d of gestation) Angus and Simmental-Angus cows [n = 46; body weight (BW) = 630 ± 12.0 kg) were blocked by breed, age, and BW and assigned to one of two treatments: 1) Ad-libitum feeding of forage-based diet (HFOR; n = 23); or 2) a concentrate corn-based diet with a restricted intake of 1.2% BW (HCON; n = 23). Cows were housed in a group-pen at the South Dakota State University Cow-Calf Education and Research Facility (Brookings, SD). Feed consumption was tracked using an electronic feeder (Insentec, The Hague, Netherlands) and enteric methane emissions were monitored using the GreenFeed trailer system (C-Lock Inc., Rapid City, SD). Body weight measurements were taken bi-weekly. Treatments started being applied on d 50 (± 10) pre-calving and will continue to be applied up to d 84 post-calving. Preliminary data reported herein was collected from d 42 pre-calving up to d 42 post-calving. Performance and dry matter intake data were analyzed as repeated measures using the MIXED procedure of SAS with treatment, period, and their interaction as fixed effects and animal as a random effect. Enteric emissions were analyzed using a linear mixed effects model with animal as a random effect in R, with a post-hoc contrast among treatments. Pre-calving, HFOR had greater (P &lt; 0.01) CH4 emissions than HCON (264 ± 9.19 g/d and 246 ± 9 g/d for HFOR and HCON, respectively). Both groups presented dynamic changes in CH4 emissions over time (P &lt; 0.01). Post-calving, no differences were observed between treatments (P = 0.55); however, temporal changes (P &lt; 0.01) and treatment × d interaction (P &lt; 0.01) persisted, indicating ongoing diet-temporal interplay. No treatment × period interactions (P ≥ 0.16) were observed for DMI and performance. As designed, dry matter intake was less (P &lt; 0.01) for HCON (8.66 ± 0.45 kg) than HFOR (17.3 ± 0.45 kg) cows. Further, dry matter intake was less (P &lt; 0.01) pre-calving (11.11 ± 0.28 kg) than post-calving (14.85 ± 0.48 kg). Cows in the HFOR group were heavier (P = 0.01) than HCON (646.22 ± 14 kg vs. 593.43 ± 14 kg, respectively) likely due to ruminal fill; and as expected cows were heavier (P &lt; 0.01) pre- than post-calving, with the greatest BW observed on d 14 pre-calving (639.52 ± 9.92 kg). These data indicate that high concentrate corn-based diets may be used in dry-lot settings to optimize nutritional management and reduce methane emissions when compared with conventional high forage winter-feeding strategies.
PSVII-24 Effectiveness of hot water knife sanitation practices during beef dressing processes
Authors: Yang Xianqin , Tran Frances , Chhabra Prakriti , Platter Zane , Tennant Travis C , Brown Tyson , Lawrence Ty E 
Published: September 2024

Abstract
Our objective was to assess whether the harvest processing equipment sanitation practices in beef processing plants are sufficient to mitigate pathogen transfer. Multiple regulatory agencies across the globe require that harvest equipment be sanitized using hot water at a minimum of 82°C, with a momentary dip when a time is specified. Consequently, hot water sanitizers have been widely implemented at meat processing facilities, in particular, for knife sanitation throughout the dressing process. However, there is no standard time requirement for hot water immersion and, as such, the effectiveness of hot water sanitization is uncertain. The practice for knife sanitation at a large beef processing facility was audited and the microbial reduction that simulated the audited practice was assessed along with a momentary dip at 82°C. A five-strain cocktail of Escherichia coli and 11 knives of differing degree of usage obtained from the beef plant were used in the study. An area of 50 cm2 of the upper surface of each knife was spread-inoculated with the five-strain E. coli cocktail and the knife was subsequently submerged in a water bath coupled to a water circulator for immersion at 82°C. Exposures times tested were those derived from the in-plant audit for each knife type. A knife that was similarly inoculated, but was not treated with hot water, served as a positive control. E. coli surviving the treatment were enumerated by plating. In addition, two knives that had most and least reduction of E. coli were artificially contaminated with meat prior to inoculation, by passing the knife through meat multiple times. For each condition, three independent trials were conducted. Least-squares means for log counts were separated by a post-hoc Tukey test. Mean immersion times for different knives audited ranged from 1 to 9.8 s. Immersing knives in water at 82°C significantly reduced the number of E. coli (P &lt; 0.05; Table 1), ranging from 2.3 (air knife)-5.5 (skinning knife 2) log units. Variation among the same knife type was observed. Momentary dip at 82°C reduced E. coli on de-boning, hook, air knife and wizard knife by 1-1.2 log (P &lt; 0.05) and &lt; 1 log for the other six knives (P ≥ 0.05). Treatment at 82°C for the duration of average exposure time observed during the in-plant audit of knives that had been contaminated with meat debris showed lower reductions of E. coli, compared with clean knives. Those reductions (&gt;1.5 log units) were significant (P &lt; 0.05). In conclusion, the duration for hot water sanitation of knives at beef processing plants achieves significant reduction of E. coli if temperature is maintained at 82°C and a momentary dip at the same temperature would be less likely to be effective.
PSI-30 Effects of high canola meal diet with acidifiers supplementation on cecal microbiota of nursery pigs
Authors: McClellan Katlyn A , Fowler Emily , Perez-Palencia Jorge Y , St-Pierre Benoit , Levesque Crystal L , Hong Jinsu 
Published: September 2024

Abstract
Inclusion of canola meal (CM) in swine diets is typically limited stemming from the presence of glucosinolates (Gls), which can reduce growth performance and cause thyroid dysfunction in nursery pigs. While certain members of hindgut bacterial communities are capable of degrading dietary Gls, which would mitigate their detrimental effects, they have yet to be identified. Moreover, lowering the pH in the hindgut of the pig can break down Gls into less toxic compounds, potentially reducing harm. Thus, this study aimed to characterize major bacterial species populating the hindgut of nursery pigs fed a high CM diet supplemented with acidifier and encapsulated butyrate. A total of 315 nursery pigs weaned at 21 d old, average body weight (BW) 6.0 ± 1.2 kg, were assigned to one of five dietary treatments with 9 replicate pens (7 pigs/pen) in a randomized complete block design. The dietary treatments included PC: a corn-soybean meal-based diet with 20% CM, NC: a corn-soybean meal-based diet with 40% CM, NC+A: NC diet supplemented with an acidifier at 1,500 ppm, NC+B: NC diet supplemented with encapsulated butyrate at 500 ppm, and NC+A+B: NC diet supplemented with both an acidifier and encapsulated butyrate. All diets were formulated to meet or exceed the nutrient requirements of NRC (2012). At the end of the feeding trial (6 wk), one pig from each pen was euthanized to collect fresh cecal digesta. Samples were analyzed using the 16S rRNA gene through PCR-amplification of the V1-V3 regions from microbial genomic DNA, followed by Illumina MiSeq 2X300 sequencing. A comparative analysis of the highly represented taxonomic groups and operational taxonomic units (OTU) using non-parametric Kruskal-Wallis sum-rank test and Wilcoxon pairwise test identified candidate bacterial groups or species enriched across different diets. Bacillota was the predominant phylum across all diets, ranging from 83.2% to 86.3%. At the genus level, Lachnospiracea incertae sedis was less abundant in the NC diets with acidifier (Acidifier effect, P &lt; 0.05), whereas Dialister was less in the NC diets with encapsulated butyrate (Butyrate effect, P &lt; 0.05). The OTU Ssd-00001 (Lactobacillus amylovorus, 99.6%) tended to be greater in the cecal microbiota of pigs fed the NC diets with acidifier (Acidifier effect, P = 0.09). Interestingly, OTU Ssd-00188 (Agathobacter rectalis, 99.2%) tended to be higher in PC diet than in NC diet (P = 0.06), while its abundance was less in the cecal microbiota of pigs fed the NC diets with acidifier (Acidifier effect, P = 0.09). In conclusion, these results provide valuable insights into the microbiome of pigs fed high CM diets. Understanding the interplay between microbial communities and high CM diet with acidifier supplementation is crucial for optimizing swine diets and mitigating challenges associated with the antinutritional effects of dietary Gls from CM.