bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated analysis reveals candidate mRNA and their potential roles in uterine leiomyomas
Authors: Xia Liping , Liu Yan , Fu Yan , Dongye Shengyi , Wang Dewei 
Published: December 2016

AbstractAimUterine leiomyomas (UL) are the most common pelvic tumors, and the etiology and pathophysiology are not well understood. We aimed to elucidate the genes responsible for UL development.MethodsIntegrated analyses of four datasets of mRNA profiling for UL were performed. Functional annotation of differentially expressed genes (DEG) was used to systematically characterize the global expression profiles. The UL‐specific protein–protein interaction network was constructed.ResultsIntegrated analysis led to the discovery of 2167 DEG (1042 upregulated and 1125 downregulated). The aberrant expression of NAV2, KIF5C, DCX, CAPN6, COL4A2, ALDH1A1, and DPT may play important roles in UL tumorigenesis. In addition, the dysregulation of MEST, LGALS3, and TLR3 may be involved in the progression of UL by a common mechanism. Functional annotation showed that extracellular matrix receptor interaction may be more active and cause the extracellular matrix abnormally formed in UL. Moreover, focal adhesion and cell adhesion molecules may play roles in the development of UL. Furthermore, chemokine signaling pathway and cytokine–cytokine receptor interaction were most probably involved in the development of UL.ConclusionIn conclusion, our study observed that a set of aberrantly expressed genes and the related biochemical pathways may lead to transformation of normal myometrium in pathological focuses.
Gestational choriocarcinoma: Rare spinal metastasis during a viable pregnancy
Authors: Ishiguro Tatsuya , Serikawa Takehiro , Yahata Tetsuro , Enomoto Takayuki 
Published: December 2016

AbstractGestational choriocarcinoma metastasizing to the bones, especially to the spine, is extremely rare. In addition, there are few reports of choriocarcinoma during a viable pregnancy. We report a case of gestational choriocarcinoma that metastasized to the lumbar spine during a viable pregnancy in a 41‐year‐old woman with a history of a missed abortion. A heterogeneous cervical mass was detected at gestational week 16. Subsequently, a metastatic lesion appeared during the pregnancy, and fetal demise in utero occurred. Pathological examination revealed that the cervical tumor and metastatic spinal tumor were choriocarcinoma. The patient's condition deteriorated rapidly and we were unable to save her life, despite multidrug chemotherapy. Surgical tumor resection and pregnancy might involve a substantial risk of choriocarcinoma metastasis. It is important to obtain an early diagnosis for this life‐threatening disease in order to facilitate appropriate treatment, despite pregnancy.
Echocardiography findings in a case with Ballantyne syndrome
Authors: Suzuki Yutaro , Yamamura Mie , Kikuchi Keisuke , Hattori Rifumi , Umazume Takeshi , Minakami Hisanori 
Published: December 2016

AbstractMarked fluid retention occurs in Ballantyne syndrome, but few reports are available on changes in cardiac morphology in this syndrome. A woman with generalized edema, dyspnea, fetal hydrops (skin edema and ascites), thickened placenta, and elevated plasma B‐type natriuretic peptide level (344 pg/mL) was admitted to our hospital at gestational week (GW) 20+3. Blood pressure remained within the normal range. However, acute increases in left atrial volume index, pulmonary artery systolic pressure, and hyperdynamic left ventricular function (as evidenced by increased left ventricular ejection fraction to 74% with cardiac index of 5.1 L/min/m2) occurred preceding fetal death at GW 21+4 in the presence of increased inferior vena cava diameter (23 mm) and relatively low systemic vascular resistance of 752 dyn·s/cm5. These findings suggested life‐threatening heart failure and required cesarean delivery at GW 21+5 resulting in complete recovery. The placenta suggested cytomegalovirus infection.
Retrospective study of tadalafil for fetal growth restriction: Impact on maternal and perinatal outcomes
Authors: Kubo Michiko , Umekawa Takashi , Maekawa Yuka , Tanaka Hiroaki , Nii Masafumi , Murabayashi Nao , Osato Kazuhiro , Kamimoto Yuki , Ikeda Tomoaki 
Published: December 2016

AbstractAimThe aim of this retrospective study was to assess tadalafil treatment in pregnant women with fetal growth restriction (FGR) in terms of maternal and perinatal outcomes.MethodsWe retrospectively analyzed 11 Japanese singleton pregnant women with FGR who received tadalafil along with conventional management for FGR at Mie University Hospital from July 2015 to February 2016 (tadalafil group). These women were matched for maternal age, parity, gestational age, and estimated fetal weight at enrollment with 14 singleton pregnant women who received only the conventional management for FGR in 2014 (conventional management group). The conventional management for FGR was performed according to guidelines for obstetric practice in Japan.ResultsBoth birthweight and fetal growth velocity from enrollment to birth were significantly higher in the tadalafil group than in the conventional management group. The cesarean delivery rate was approximately twofold higher in the conventional management group than in the tadalafil group. Importantly, cesarean section due to non‐reassuring fetal status was performed in seven pregnant women in the conventional management group (58.3%) but in none in the tadalafil group (P < 0.05, chi‐squared test).ConclusionsTadalafil may improve perinatal outcome in FGR by modulating fetal growth through maintenance or improvement of fetal well‐being.
Bringing work stress home: The impact of role conflict and role overload on spousal marital satisfaction
Authors: Carnes Andrew M. 
Published: December 2016

Many previous studies have explored the possibility that work stressors negatively impact the well‐being of an individual's spouse with little success. This study aimed to extend the existing literature by exploring boundary conditions of the relationship including the possibility that political skill and gender modify the relationship between role conflict, role overload, and spousal marital satisfaction. It also suggests that men and women differ in their propensity to cope with different types of stressors. Using a sample of 139 matched marital dyads, analytical results generally support these hypotheses. Political skill was a significant moderator of the role conflict–marital satisfaction relationship for the husband‐to‐wife transaction, but not the wife‐to‐husband transaction. In addition, political skill was a significant moderator of the role overload–marital satisfaction relationship for the wife‐to‐husband transaction but not the husband‐to‐wife transaction. These findings are important to both research and practice in that they highlight the issue of stress crossover and the importance of coping in the workplace.Practitioner points

It is advantageous for an employee's marital well‐being to deal with work stressors using work‐related resources.

Males tend to use coping resources to address role conflict, while females divert these resources to role overload.
SNP‐Based Heritability Estimates of Common and Specific Variance in Self‐ and Informant‐Reported Neuroticism Scales
Authors: Realo Anu , van der Most Peter J. , Allik Jüri , Esko Tõnu , Jeronimus Bertus F. , Kööts‐Ausmees Liisi , Mõttus René , Tropf Felix C. , Snieder Harold , Ormel Johan 
Published: December 2016

AbstractObjectiveOur study aims to estimate the proportion of the phenotypic variance of Neuroticism and its facet scales that can be attributed to common single‐nucleotide polymorphisms (SNPs) in two adult populations from Estonia (EGCUT; N = 3,292) and the Netherlands (Lifelines; N = 13,383).MethodGenomic‐relatedness‐matrix restricted maximum likelihood (GREML) using genome‐wide complex trait analysis (GCTA) software was employed. To build upon previous research, we used self‐ and informant reports of the 30‐facet NEO personality inventories and analyzed both the usual sum scores and the residual facet scores of Neuroticism.ResultsIn the EGCUT cohort, the proportion of phenotypic variance explained by the additive effects of common genetic variants in self‐ and informant‐reported Neuroticism domain scores was 15.2% (p = .070, SE = .11) and 6.2% (p = .293, SE = .12), respectively. The SNP‐based heritability estimates at the level of Neuroticism facet scales differed greatly across cohorts and modes of measurement but were generally higher (a) for self‐ than for informant reports, and (b) for sum than for residual scores.ConclusionsOur findings indicate that a large proportion of the heritability of Neuroticism is not captured by additive genetic effects of common SNPs, with some evidence for Gene × Environment interaction across cohorts.
Sharing the Now in the Social Present: Duration of Nonverbal Synchrony Is Linked With Personality
Authors: Tschacher Wolfgang , Ramseyer Fabian , Koole Sander L. 
Published: December 2016

AbstractObjectiveThe social present is a novel descriptor of dyadic nowness and social sharing, extending research on individual nowness (James's [1890] specious present) to the interpersonal and intersubjective domain. We wished to connect this descriptor to personality attributes.MethodWe define the social present by the duration of significant nonverbal synchrony, based on the phenomenon of movement synchrony that generally emerges in social interactions. It is thus an implicit and objective measure that can be implemented by automated video analyses. In this study, 168 healthy participants were invited to verbal conversations in same‐sex dyads. We analyzed the associations of the social present with personality attributes and interaction types (competition, cooperation, fun task).ResultsThe average duration of the social present was 6.0 seconds, highest in competitive interactions and in male‐male dyads. People with higher Openness to Experience, higher avoidant attachment, and lower narcissistic interpersonal styles showed extended social present in their interactions.ConclusionsThe concept of the social present extends personality attributes to the interpersonal domain and to intersubjectivity. The social present may be computed based on movement synchrony but also prosodic or physiological synchronies. We foresee implications for health‐related interactions such as psychotherapy, where therapeutic presence is an essential property of alliance.
Experiences of patients undergoing dialysis who are from ethnic and racial minorities
Authors: Nagpal Neha , Boutin‐Foster Carla , Melendez Jennifer , Kubiszeswki Patryk , Uehara Kamalani , Offidani Emanuela , Faussett Zenobia , Chen Richie , Redel Cathy , Waltrous Clarence , Smith Barry 
Published: December 2016

SUMMARYBackgroundApproximately 500,000 people in the United States are affected by end‐stage kidney disease (ESKD), 53% of whom are Black or Latino. ESKD significantly impacts psychosocial health and quality of life. However, few studies address the psychosocial aspects of ESKD, especially among black and Latino adults. This study sought to understand the psychosocial context of living with ESKD among black and Latino adults who reside in a medically underserved community.Study DesignA qualitative study.Setting and ParticipantsParticipants were recruited from a dialysis centre in East New York, Brooklyn, a medically underserved community.MethodologyDescriptive phenomenology was used as a qualitative approach for capturing the experiences of patients who received dialysis in this community.Analytical ApproachOpen‐ended interviews were audio‐taped, transcribed, coded and analysed using standard qualitative techniques.ResultsData saturation was achieved at 36 participants. The following five themes emerged: the transition to dialysis is abrupt and unexpected; denial is often an initial response; dialysis is the new normal and in order to survive one must forget the past and press forward; dialysis changes everything and impacts the entire family; strength was often found in faith and family.LimitationsThis study was conducted in one setting and may need to be expanded to other sites to capture the experiences of patients cared for in other settings.ConclusionThese findings have practical implications for informing patient‐centered models of care that are more responsive to the psychosocial needs of patients with ESKD living in medically underserved communities.
Differences in background characteristics of patients with chronic hepatitis C who achieved sustained virologic response with interferon‐free versus interferon‐based therapy and the risk of developing hepatocellular carcinoma after eradication of hepatitis C virus in Japan
Authors: Toyoda H. , Tada T. , Takaguchi K. , Senoh T. , Shimada N. , Hiraoka A. , Michitaka K. , Ishikawa T. , Kumada T. 
Published: December 2016

SummaryWe compared the background characteristics of patients with chronic hepatitis C who achieved eradication of hepatitis C virus (HCV), that is sustained virologic response (SVR), with interferon (IFN)‐based versus IFN‐free antiviral therapy in Japan. In addition, we used a previously reported risk assessment model to compare the incidence of hepatocellular carcinoma (HCC) after SVR by treatment type. Pretreatment characteristics of 1533 patients who achieved SVR with IFN‐based therapy and 1086 patients with IFN‐free therapy from five institutions across Japan were compared. The risk of HCC after SVR was assessed based on pretreatment characteristics, and the incidence of HCC after SVR was estimated in both groups. Age and serum alpha‐fetoprotein levels were higher, platelet count was lower, and liver fibrosis was more advanced in patients who achieved SVR with IFN‐free therapy compared with IFN‐based therapy. The incidence of HCC after SVR in the IFN‐free group was estimated to be more than twofold higher than in the IFN‐based therapy group (7.29% vs. 3.09%, and 6.23% vs. 3.01% when excluding patients who have underwent curative treatment for HCC). There are large differences in pretreatment characteristics between patients who achieved SVR with IFN‐based and IFN‐free therapies in Japan, which are associated with differential risk of HCC after SVR. These differences can influence the incidence of HCC after SVR and should be taken into consideration when comparing IFN‐based and IFN‐free therapies in terms of hepatocarcinogenesis suppression with HCV eradication.
Macrofaunal nematodes of the deep Whittard Canyon (NE Atlantic): assemblage characteristics and comparison with polychaetes
Authors: Gunton Laetitia M. , Bett Brian J. , Gooday Andrew J. , Glover Adrian G. , Vanreusel Ann 
Published: December 2016

AbstractLarge nematodes form an important component of deep‐sea macrofaunal assemblages, but are often considered to be part of the meiobenthos and are rarely studied. We analysed the standing stocks, diversity, and functional group and genus‐level composition of macrofaunal nematodes at lower bathyal depths (3,500 m water depth) in the Whittard Canyon system (NE Atlantic) and on the adjacent continental slope. Five replicate sample sets were obtained using a Megacorer, at four locations (three canyon branches, one slope). Sediment samples were processed on a 500‐μm mesh to provide both nematode and polychaete data from the same samples. The dominant nematode genera included Paramesacanthion, Metacylicolaimus, Cylicolaimus and Phanodermopsis. Nematode standing stocks (density and biomass) increased significantly from slope to canyon locations. Similarly, nematode dominance increased substantially (and diversity decreased) from slope to canyon locations. Nematode feeding groups and tail shape groups also appeared to exhibit common trends across study locations. Nematode genus‐level faunal composition varied significantly between slope and canyon locations. We describe and discuss the broadly similar trends detected in the matching polychaete data, noting some differences in the polychaete density and diversity responses. We suggest that the similar trends in macrofaunal nematode and polychaete ecology across our four study locations reflect responses to both changing sedimentology and food availability.
Iodine status of pregnant women in South Australia after mandatory iodine fortification of bread and the recommendation for iodine supplementation
Authors: Condo Dominique , Huyhn Dao , Anderson Amanda J. , Skeaff Sheila , Ryan Philip , Makrides Maria , Mühlhaüsler Beverly S. , Zhou Shao J. 
Published: December 2016

AbstractMandatory iodine fortification of bread was introduced in 2009 in Australia in response to the reemergence of iodine deficiency. The aim of this study was to assess iodine intake, urinary iodine concentration (UIC) and their correlation in pregnant women (n = 783) recruited from South Australia 2 years following mandatory iodine fortification. Total iodine intake (food and supplements) and UIC were assessed at study entry (<20 weeks') and at 28 weeks' gestation. Mean (±SD) total iodine intake at study entry and 28 weeks' gestation was 307 ± 128 μg/day and 300 ± 127 μg/day, respectively. Overall, 85.9% of women met the estimated average intake (≥160 μg/day) for iodine in pregnancy, but only 44.5% met the estimated average intake from food alone. The main food sources of iodine were dairy foods and iodine‐fortified bread. Median (interquartile range) UIC at study entry and 28 weeks' gestation was 189 μg/L and 172 μg/L, respectively. At study entry, median UIC was higher in women taking supplements containing iodine ≥150 μg/day compared with those containing iodine <150 μg/day (221 μg/L vs. 163 μg/L, p = .003) and those not taking supplements containing iodine (221 μg/L vs. 159 μg/L, p < .001). At 28 weeks' gestation, the median UIC for the groups was 187, 152 and 141 μg/L, respectively (each of the two comparisons yielded p < .001). Total iodine intake (food and supplements) from all women was positively, though weakly, correlated with UIC (r = .23, p < .001). In conclusion, pregnant women in South Australia are iodine sufficient postmandatory iodine fortification of bread. However, without iodine supplementation, it may be difficult to achieve a UIC >150 μg/L.
Genomic evolution of bacterial populations under coselection by antibiotics and phage
Authors: Cairns Johannes , Frickel Jens , Jalasvuori Matti , Hiltunen Teppo , Becks Lutz 
Published: December 2016

AbstractBacteria live in dynamic systems where selection pressures can alter rapidly, forcing adaptation to the prevailing conditions. In particular, bacteriophages and antibiotics of anthropogenic origin are major bacterial stressors in many environments. We previously observed that populations of the bacterium Pseudomonas fluorescens SBW25 exposed to the lytic bacteriophage SBW25Φ2 and a noninhibitive concentration of the antibiotic streptomycin (coselection) achieved higher levels of phage resistance compared to populations exposed to the phage alone. In addition, the phage became extinct under coselection while remaining present in the phage alone environment. Further, phenotypic tests indicated that these observations might be associated with increased mutation rate under coselection. In this study, we examined the genetic causes behind these phenotypes by whole‐genome sequencing clones isolated from the end of the experiments. We were able to identify genetic factors likely responsible for streptomycin resistance, phage resistance and hypermutable (mutator) phenotypes. This constitutes genomic evidence in support of the observation that while the presence of phage did not affect antibiotic resistance, the presence of antibiotic affected phage resistance. We had previously hypothesized an association between mutators and elevated levels of phage resistance under coselection. However, our evidence regarding the mechanism was inconclusive, as although with phage mutators were only found under coselection, additional genomic evidence was lacking and phage resistance was also observed in nonmutators under coselection. More generally, our study provides novel insights into evolution between univariate and multivariate selection (here two stressors), as well as the potential role of hypermutability in natural communities.
Model‐based inference from microvascular measurements: Combining experimental measurements and model predictions using a Bayesian probabilistic approach
Authors: Rasmussen Peter M. , Smith Amy F. , Sakadžić Sava , Boas David A. , Pries Axel R. , Secomb Timothy W. , Østergaard Leif 
Published: December 2016

ABSTRACTObjectiveIn vivo imaging of the microcirculation and network‐oriented modeling have emerged as powerful means of studying microvascular function and understanding its physiological significance. Network‐oriented modeling may provide the means of summarizing vast amounts of data produced by high‐throughput imaging techniques in terms of key, physiological indices. To estimate such indices with sufficient certainty, however, network‐oriented analysis must be robust to the inevitable presence of uncertainty due to measurement errors as well as model errors.MethodsWe propose the Bayesian probabilistic data analysis framework as a means of integrating experimental measurements and network model simulations into a combined and statistically coherent analysis. The framework naturally handles noisy measurements and provides posterior distributions of model parameters as well as physiological indices associated with uncertainty.ResultsWe applied the analysis framework to experimental data from three rat mesentery networks and one mouse brain cortex network. We inferred distributions for more than 500 unknown pressure and hematocrit boundary conditions. Model predictions were consistent with previous analyses, and remained robust when measurements were omitted from model calibration.ConclusionOur Bayesian probabilistic approach may be suitable for optimizing data acquisition and for analyzing and reporting large data sets acquired as part of microvascular imaging studies.
Acetyl‐CoA synthetases of Saccharopolyspora erythraea are regulated by the nitrogen response regulator GlnR at both transcriptional and post‐translational levels
Authors: You Di , Wang Miao‐Miao , Ye Bang‐Ce 
Published: December 2016

SummarySaccharopolyspora erythraea has three AMP‐forming acetyl‐CoA synthetases (Acs) encoded by acsA1, acsA2, and acsA3. In this work, we found that nitrogen response regulator GlnR can directly interact with the promoter regions of all three genes and can activate their transcription in response to nitrogen availability. The typical GlnR‐binding boxes were identified in the promoter regions. Moreover, the activities of three Acs enzymes were modulated by the reversible lysine acetylation (RLA) with acetyltransferase AcuA and NAD+‐dependent deacetylase SrtN. Interestingly, GlnR controlled the RLA by directly activating the expression of acuA and srtN. A glnR‐deleted mutant (ΔglnR) caused a growth defect in 10 mM acetate minimal medium, a condition under which RLA function is critical to control Acs activity. Overexpression of acuA reversed the growth defect of ΔglnR mutant. Total activity of Acs in cell‐free extracts from ΔglnR strain had a 4‐fold increase relative to that of wildtype strain. Western Blotting showed that in vivo acetylation levels of Acs were influenced by nitrogen availability and lack of glnR. These results demonstrated that GlnR regulated acetyl‐CoA synthetases at transcriptional and post‐translational levels, and mediated the interplay between nitrogen and carbon metabolisms by integrating nitrogen signals to modulate the acetate metabolism.