bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can the Social Vulnerability Index Be Used for More Than Emergency Preparedness? An Examination Using Youth Physical Fitness Data
Authors: Gay Jennifer L. , Robb Sara W. , Benson Kelsey M. , White Alice 
Published: March 2016

Background:The Social Vulnerability Index (SVI), a publicly available dataset, is used in emergency preparedness to identify communities in greatest need of resources. The SVI includes multiple socioeconomic, demographic, and geographic indicators that also are associated with physical fitness and physical activity. This study examined the utility of using the SVI to explain variation in youth fitness, including aerobic capacity and body mass index.Methods:FITNESSGRAM data from 2,126 Georgia schools were matched at the census tract level with SVI themes of socioeconomic, household composition, minority status and language, and housing and transportation. Multivariate multiple regression models were used to test whether SVI factors explained fitness outcomes, controlling for grade level (ie, elementary, middle, high school) and stratified by gender.Results:SVI themes explained the most variation in aerobic fitness and body mass index for both boys and girls (R2 values 11.5% to 26.6%). Socioeconomic, Minority Status and Language, and Housing and Transportation themes were salient predictors of fitness outcomes.Conclusions:Youth fitness in Georgia was related to socioeconomic, demographic, and geographic themes. The SVI may be a useful needs assessment tool for health officials and researchers examining multilevel influences on health behaviors or identifying communities for prevention efforts.
Reduced Diagnostic Performance of Two Norovirus Antigen Enzyme Immunoassays for the Emergent Genogroup II Genotype 17 Kawasaki 2014 Variant
Authors: Chan Martin C. W. , Kwok Kirsty , Hung Tin-Nok , Chan Paul K. S. 
Published: March 2016

ABSTRACT
Two commonly used norovirus enzyme immunoassays have reduced diagnostic performance, with clinical sensitivities ranging from 11% to 35% for the detection of the recently emerging genogroup II genotype 17 (GII.17) Kawasaki 2014 variant that caused the majority of infections in Asia during the winter of 2014 to 2015. False-negative results can compromise infection control and patient management.
Antimicrobial-Loaded Bone Cement Does Not Negatively Influence Sonicate Fluid Culture Positivity for Diagnosis of Prosthetic Joint Infection
Authors: Park Kyung-Hwa , Greenwood-Quaintance Kerryl E. , Hanssen Arlen D. , Abdel Matthew P. , Patel Robin 
Published: March 2016

ABSTRACT
We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cemented prosthesis and 58.3% (21 of 36) in subjects with an antimicrobial-free prosthesis.
The Coxsackievirus and Adenovirus Receptor: Glycosylation and the Extracellular D2 Domain Are Not Required for Coxsackievirus B3 Infection
Authors: Pinkert Sandra , Röger Carsten , Kurreck Jens , Bergelson Jeffrey M. , Fechner Henry 
Published: March 2016

ABSTRACT
The coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin superfamily (IgSF) and functions as a receptor for coxsackie B viruses (CVBs). The extracellular portion of CAR comprises two glycosylated immunoglobulin-like domains, D1 and D2. CAR-D1 binds to the virus and is essential for virus infection; however, it is not known whether D2 is also important for infection, and the role of glycosylation has not been explored. To understand the function of these structural components in CAR-mediated CVB3 infection, we generated a panel of human (h) CAR deletion and substitution mutants and analyzed their functionality as CVB receptors, examining both virus binding and replication. Lack of glycosylation of the CAR-D1 or -D2 domains did not adversely affect CVB3 binding or infection, indicating that the glycosylation of CAR is not required for its receptor functions. Deletion of the D2 domain reduced CVB3 binding, with a proportionate reduction in the efficiency of virus infection. Replacement of D2 with the homologous D2 domain from chicken CAR, or with the heterologous type C2 immunoglobulin-like domain from IgSF11, another IgSF member, fully restored receptor function; however, replacement of CAR-D2 with domains from CD155 or CD80 restored function only in part. These data indicate that glycosylation of the extracellular domain of hCAR plays no role in CVB3 receptor function and that CAR-D2 is not specifically required. The D2 domain may function largely as a spacer permitting virus access to D1; however, the data may also suggest that D2 affects virus binding by influencing the conformation of D1.

IMPORTANCE
An important step in virus infection is the initial interaction of the virus with its cellular receptor. Although the role in infection of the extracellular CAR-D1, cytoplasmic, and transmembrane domains have been analyzed extensively, nothing is known about the function of CAR-D2 and the extracellular glycosylation of CAR. Our data indicate that glycosylation of the extracellular CAR domain has only minor importance for the function of CAR as CVB3 receptor and that the D2 domain is not essential per se but contributes to receptor function by promoting the exposure of the D1 domain on the cell surface. These results contribute to our understanding of the coxsackievirus-receptor interactions.
Roles of Us8A and Its Phosphorylation Mediated by Us3 in Herpes Simplex Virus 1 Pathogenesis
Authors: Kato Akihisa , Ando Tomoko , Oda Shinya , Watanabe Mizuki , Koyanagi Naoto , Arii Jun , Kawaguchi Yasushi 
Published: March 2016

ABSTRACT
The herpes simplex virus 1 (HSV-1) Us8A gene overlaps the gene that encodes glycoprotein E (gE). Previous studies have investigated the roles of Us8A in HSV-1 infection using null mutations in Us8A and gE; therefore, the role of Us8A remains to be elucidated. In this study, we investigated the function of Us8A and its phosphorylation at serine 61 (Ser-61), which we recently identified as a phosphorylation site by mass spectrometry-based phosphoproteomic analysis of HSV-1-infected cells, in HSV-1 pathogenesis. We observed that (i) the phosphorylation of Us8A Ser-61 in infected cells was dependent on the activity of the virus-encoded Us3 protein kinase; (ii) the Us8A null mutant virus exhibited a 10-fold increase in the 50% lethal dose for virulence in the central nervous system (CNS) of mice following intracranial infection compared with a repaired virus; (iii) replacement of Ser-61 with alanine (S61A) in Us8A had little effect on virulence in the CNS of mice following intracranial infection, whereas it significantly reduced the mortality of mice following ocular infection to levels similar to the Us8A null mutant virus; (iv) the Us8A S61A mutation also significantly reduced viral yields in mice following ocular infection, mainly in the trigeminal ganglia and brains; and (v) a phosphomimetic mutation at Us8A Ser-61 restored wild-type viral yields and virulence. Collectively, these results indicate that Us8A is a novel HSV-1 virulence factor and suggest that the Us3-mediated phosphorylation of Us8A Ser-61 regulates Us8A function for viral invasion into the CNS from peripheral sites.

IMPORTANCE
The DNA genomes of viruses within the subfamily
Alphaherpesvirinae
are divided into unique long (UL) and unique short (Us) regions. Us regions contain alphaherpesvirus-specific genes. Recently, high-throughput sequencing of ocular isolates of HSV-1 showed that Us8A was the most highly conserved of 13 herpes simplex virus 1 (HSV-1) genes mapped to the Us region, suggesting Us8A may have an important role in the HSV-1 life cycle. However, the specific role of Us8A in HSV-1 infection remains to be elucidated. Here, we show that Us8A is a virulence factor for HSV-1 infection in mice, and the function of Us8A for viral invasion into the central nervous system from peripheral sites is regulated by Us3-mediated phosphorylation of the protein at Ser-61. This is the first study to report the significance of Us8A and its regulation in HSV-1 infection.
Differential Inductions of RNA Silencing among Encapsidated Double-Stranded RNA Mycoviruses in the White Root Rot Fungus Rosellinia necatrix
Authors: Yaegashi Hajime , Shimizu Takeo , Ito Tsutae , Kanematsu Satoko 
Published: March 2016

ABSTRACT

RNA silencing acts as a defense mechanism against virus infection in a wide variety of organisms. Here, we investigated inductions of RNA silencing against encapsidated double-stranded RNA (dsRNA) fungal viruses (mycoviruses), including a partitivirus (RnPV1), a quadrivirus (RnQV1), a victorivirus (RnVV1), a mycoreovirus (RnMyRV3), and a megabirnavirus (RnMBV1) in the phytopathogenic fungus
Rosellinia necatrix
. Expression profiling of RNA silencing-related genes revealed that a dicer-like gene, an Argonaute-like gene, and two RNA-dependent RNA polymerase genes were upregulated by RnMyRV3 or RnMBV1 infection but not by other virus infections or by constitutive expression of dsRNA in
R. necatrix
. Massive analysis of viral small RNAs (vsRNAs) from the five mycoviruses showed that 19- to 22-nucleotide (nt) vsRNAs were predominant; however, their ability to form duplexes with 3′ overhangs and the 5′ nucleotide preferences of vsRNAs differed among the five mycoviruses. The abundances of 19- to 22-nt vsRNAs from RnPV1, RnQV1, RnVV1, RnMyRV3, and RnMBV1 were 6.8%, 1.2%, 0.3%, 13.0%, and 24.9%, respectively. Importantly, the vsRNA abundances and accumulation levels of viral RNA were not always correlated, and the origins of the vsRNAs were distinguishable among the five mycoviruses. These data corroborated diverse interactions between encapsidated dsRNA mycoviruses and RNA silencing. Moreover, a green fluorescent protein (GFP)-based sensor assay in
R. necatrix
revealed that RnMBV1 infection induced silencing of the target sensor gene (GFP gene and the partial RnMBV1 sequence), suggesting that vsRNAs from RnMBV1 activated the RNA-induced silencing complex. Overall, this study provides insights into RNA silencing against encapsidated dsRNA mycoviruses.

IMPORTANCE
Encapsidated dsRNA fungal viruses (mycoviruses) are believed to replicate inside their virions; therefore, there is a question of whether they induce RNA silencing. Here, we investigated inductions of RNA silencing against encapsidated dsRNA mycoviruses (a partitivirus, a quadrivirus, a victorivirus, a mycoreovirus, and a megabirnavirus) in
Rosellinia necatrix
. We revealed upregulation of RNA silencing-related genes in
R. necatrix
infected with a mycoreovirus or a megabirnavirus but not with other viruses, which was consistent with the relatively high abundances of vsRNAs from the two mycoviruses. We also showed common and different molecular features and origins of the vsRNAs from the five mycoviruses. Furthermore, we demonstrated the activation of RNA-induced silencing complex by mycoviruses in
R. necatrix
. Taken together, our data provide insights into an RNA silencing pathway against encapsidated dsRNA mycoviruses which is differentially induced among encapsidated dsRNA mycoviruses; that is, diverse replication strategies exist among encapsidated dsRNA mycoviruses.
Increased Incidence of Second Malignancies Associated with Small Bowel Adenocarcinoma
Authors: Ripley Dianne , Weinerman Brian H 
Published: March 2016

BACKGROUND:Some data suggest that there is an increased incidence of second malignancies associated with small bowel adenocarcinomas, but this has not been reviewed in the context of a tumour registry.OBJECTIVE:To review tumour registries based on population statistics to determine whether there is an increased incidence of second malignancies associated with small bowel adenocarcinomas.METHODS:The authors reviewed the tumour registries of two Canadian provinces (British Columbia and Manitoba) for small bowel adenocarcinoma to determine whether an increase in associated malignancies existed compared with those expected in the respective populations.RESULTS:A greater than eightfold increase in second malignancies was associated with small bowel carcinoma. The majority (73%) occurred before the diagnosis of the small bowel malignancy. Twenty-nine per cent were associated with cancers of the colon, rectum or both.CONCLUSIONS:There is an increased association of malignancy and the diagnosis of small bowel cancer. Generally, small bowel cancer is the second malignancy to be diagnosed, and the diagnosis is most often made in the elderly. Does this represent a syndrome related to an unstable gene (or genes) or a lack of repair, which makes individuals susceptible to this malignancy as they age?
A GRP78-Directed Monoclonal Antibody Recaptures Response in Refractory Multiple Myeloma with Extramedullary Involvement
Authors: Rasche Leo , Menoret Emmanuelle , Dubljevic Valentina , Menu Eline , Vanderkerken Karin , Lapa Constantin , Steinbrunn Torsten , Chatterjee Manik , Knop Stefan , Düll Johannes , Greenwood Deanne L. , Hensel Frank , Rosenwald Andreas , Einsele Hermann , Brändlein Stephanie 
Published: March 2016

Abstract
Purpose: Glucose-regulated protein (GRP) 78 is overexpressed in multiple myeloma, and both its surface expression and its biologic significance as key sensor of the unfolded protein response make GRP78 an ideal candidate for immunotherapeutic intervention. The monoclonal antibody PAT-SM6 targets surface GRP78 and leads to disease stabilization when used as single agent in a clinical trial. In this article, we evaluated expression of GRP78 in relapsed-refractory disease and explored PAT-SM6 therapy in combination regimens.
Experimental Design: GRP78 expression was immunohistochemically analyzed during disease progression and development of drug resistance throughout different stages of multiple myeloma. Activity of PAT-SM6 was evaluated in combination with anti–multiple myeloma agents lenalidomide, bortezomib, and dexamethasone in vitro. Finally, we report on a multiple myeloma patient with relapsed-refractory disease treated with PAT-SM6 in combination with bortezomib and lenalidomide.
Results: Although sGRP78 expression was present at all stages, it increased with disease progression and was even strongly elevated in patients with drug-resistant and extramedullary disease. Pretreatment with dexamethasone as well as dual combination of PAT-SM6/lenalidomide further increased sGRP78 expression and consecutively showed synergistic anti–multiple myeloma effects with PAT-SM6 in proliferation assays. As proof of concept, a 62-year-old male with triple resistant multiple myeloma treated with PAT-SM6, bortezomib, and lenalidomide experienced partial remission of both intra- and extramedullary lesions.
Conclusions: PAT-SM6 therapy in combination regimens showed efficacy in relapsed-refractory multiple myeloma. Clin Cancer Res; 22(17); 4341–9. ©2016 AACR.
Induced Voltages on a Gas Pipeline due to Lightning Strikes on Nearby Overhead Transmission Line
Authors: Elgayar Ali I , Abdul-Malek Zulkurnain 
Published: March 2016

<span lang="EN-US">The purpose of this paper is to investigate the severity of lightning induced voltages on a gas pipeline installed in parallel with overhead transmission line using two different simulation packages. The results from this study using CDEGS, which solves a given problem based on electromagnetic computations, reveal that the induced voltages on the pipeline are more accurate compared to that obtained by PSCAD simulation, which is based on the circuit approach. Unlike PSCAD, CDEGS considers many salient factors such as soil model, inductive, capacitive and conductive couplings, and multiple soil structures. Models of a double circuit 132kV transmission line, gas pipelines, soil with different resistivities and variable lightning surges were developed. The effects of pipelines located at various heights above ground and distance of pipeline from the power lines were also studied. Compared to previously published work using PSCAD, it is found that CDEGS has given more accurate results. Several findings which were not possible using PSCAD were observed such as the effect of soil structure on induced voltage and multiple layers soil. This also led to better understanding of the conductive coupling from lightning strikes and fault conditions. The modeling work using CDEGS not only useful for providing more reliable data for further protection and mitigation techniques, but is also very versatile to study the effects of various other important factors affecting the induced voltage on the pipelines. </span>
The Feedback-related Negativity Codes Components of Abstract Inference during Reward-based Decision-making
Authors: Reiter Andrea M. F. , Koch Stefan P. , Schröger Erich , Hinrichs Hermann , Heinze Hans-Jochen , Deserno Lorenz , Schlagenhauf Florian 
Published: March 2016

Abstract
Behavioral control is influenced not only by learning from the choices made and the rewards obtained but also by “what might have happened,” that is, inference about unchosen options and their fictive outcomes. Substantial progress has been made in understanding the neural signatures of direct learning from choices that are actually made and their associated rewards via reward prediction errors (RPEs). However, electrophysiological correlates of abstract inference in decision-making are less clear. One seminal theory suggests that the so-called feedback-related negativity (FRN), an ERP peaking 200–300 msec after a feedback stimulus at frontocentral sites of the scalp, codes RPEs. Hitherto, the FRN has been predominantly related to a so-called “model-free” RPE: The difference between the observed outcome and what had been expected. Here, by means of computational modeling of choice behavior, we show that individuals employ abstract, “double-update” inference on the task structure by concurrently tracking values of chosen stimuli (associated with observed outcomes) and unchosen stimuli (linked to fictive outcomes). In a parametric analysis, model-free RPEs as well as their modification because of abstract inference were regressed against single-trial FRN amplitudes. We demonstrate that components related to abstract inference uniquely explain variance in the FRN beyond model-free RPEs. These findings advance our understanding of the FRN and its role in behavioral adaptation. This might further the investigation of disturbed abstract inference, as proposed, for example, for psychiatric disorders, and its underlying neural correlates.
Umberto Eco’s Intertextuality: Appropriation of Dostoevsky’s Writing
Authors: Aloe Stefano 
Published: March 2016

The starting point for this analysis is a statement by Umberto Eco in his essay "The American Myth in Three Anti-American Generations", where, with characteristic irony, he says: "If I reflect on my spiritual development, in the list of sources I should include Imitation of Christ, No no Nanette (the song), Dostoevsky and Donald Duck".
In this playful list of creations from both high and popular culture, from Thomas Kempis to Walt Disney, the place of Dostoevsky is pivotal: a link connecting two cultural dimensions that are seemingly opposite. Dostoevsky reaches incredible depths of moral and philosophical analysis as high literature, while retaining those features that make the popular novel a literary genre of broad reach.
Highly Significant Responses to Anthropogenic Forcings of the Midlatitude Jet in the Southern Hemisphere
Authors: Solomon Abraham , Polvani L. M. 
Published: March 2016

Abstract
It has been suggested that changes in the atmospheric circulation caused by anthropogenic forcings are highly uncertain, owing to the large natural variability intrinsic to the system. Here, to assess the statistical significance of such changes for the midlatitude, large-scale atmospheric circulation of the Southern Hemisphere, a new 40-member ensemble of integrations, from 1920 to 2080, of the Community Earth System Model, version 5, is analyzed together with a companion 1800-yr-long preindustrial control integration of the same fully coupled model. For simplicity, only the latitudinal position and the strength of the zonal-mean eddy-driven jet are considered. Given the large year-to-year variability of these jet properties, this paper focuses on their decadal averages, which reflect the more slowly varying climate state. The analysis herein reveals that the forced response in such decadal averages easily emerges from the natural variability, with only a few model integrations typically needed to establish statistical significance. In particular, a forced summertime poleward shift of the jet in the latter part of the twentieth century and a strengthening of the jet during the twenty-first century in all seasons of the year are found. Contrasting these with changes in the southern annular mode, this confirms earlier studies demonstrating that such a mode is unable to distinguish different structural changes in the midlatitude jet.
Autologous Osteochondral Transplantation for Osteochondral Lesions of the Talus
Authors: Flynn Seán , Ross Keir A. , Hannon Charles P. , Yasui Youichi , Newman Hunter , Murawski Christopher D. , Deyer Timothy W. , Do Huong T. , Kennedy John G. 
Published: March 2016

Background: Autologous osteochondral transplantation (AOT) is used to treat osteochondral lesions (OCLs) of the talus, typically reserved for lesions greater than 150 mm2. Few studies exist examining the functional and magnetic resonance imaging (MRI) outcomes following this procedure. The purpose of this study was to investigate functional and MRI outcomes, including quantitative T2 mapping following AOT. Methods: Eighty-five consecutive patients who underwent AOT were identified. Functional outcomes were assessed pre- and postoperatively using the Foot and Ankle Outcome Score (FAOS). The Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score was used to assess cartilage incorporation. Quantitative T2 MRI relaxation time of graft tissue and adjacent normal cartilage values were recorded in a subset of 61 patients. The mean clinical follow-up was 47.2 months, with mean MRI follow-up of 24.8 months. Results: Mean FAOS improved pre- to postoperatively from 50 to 81 ( P < .001). The mean MOCART score was 85.8. Lesion size was negatively correlated with MOCART score ( r = −0.36, P = .004). Superficial T2 values in graft tissue were higher than control tissue (42.0 vs 35.8, P < .001). Deep T2 values in graft tissue were similar to the control values (30.9 vs 30.0, P = .305). Functional outcomes were similar in patients irrespective of whether they had previous microfracture or concomitant procedures. Conclusion: AOT was an effective treatment for large OCLs of the talus in the current study. MOCART scoring indicated good structural integrity of the graft. Quantitative T2 mapping suggests that graft tissue may not always mirror native hyaline cartilage. The long-term implications of this are not yet known. Level of Evidence: Level IV, case series.