bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling the Power of Mitochondrial Fission and Fusion: A Five-Gene Signature for Personalized Prognosis in Gastric Cancer
Authors: Zhou Bin , Tie Ping , Li Dongbing , Lu You , Liu Yuanhua 
Published: October 2024

Background:
Mitochondrial fission and fusion play important roles in tumorigenesis,
progression and therapy. Dysregulation of these processes may lead to tumor progression,
and regulation of these processes may provide novel strategies for cancer therapy. The involvement
of genes related to mitochondrial fission and fusion (MD) in gastric cancer (GC) remains
poorly understood.

Objective:
The aim of this study was to establish an MD gene signature for GC patients and to
investigate its association with prognosis, tumor microenvironment and treatment response in
GC.

Methods:
We use the TCGA-GC database as the cohort, focusing specifically on genes associated
with MD. We conducted identification and consistency clustering analysis of differentially
expressed genes in MD, conducted MD gene mutation and copy number variation analysis,
as well as correlation and functional enrichment analysis between MD gene cluster classification
and immune infiltration. TCGA-GC and GSE15459 were used to construct training and
validation cohorts for the model. We used various statistical methods, including Cox and Lasso
regression, to develop the model. We validated the model using bulk transcriptome and single-
cell transcriptome datasets (GSE13861, GSE26901, GSE66229, and GSE13450). We
used GSEA enrichment, CIBERSORT algorithm, ESTIMATE, and TIDE to gain insight into
the annotation of MD signature and the characterization of the tumor microenvironment. OncoPredict
was used to analyze the relationship between the PRG signature and the drug sensitivity.
We validated the expression of several key genes in MD signature on GC cell lines using
quantitative real-time PCR (qRT-PCR).

Conclusion:
The MD signature has the capacity to significantly contribute to the prediction of
personalized outcomes and the advancement of novel therapeutic strategies tailored for GC patients.

Results:
These MDs-related subtypes exhibited different prognosis and immune filtration patterns.
A five-gene signature, comprising AGT, HCFC1, KIFC3, NOX4, and RIN1, was developed.
There was a clear distinction in overall survival between low- and high-risk patients.
The analyses showed further confirmation of the independent prognostic value of the gene signature.
There was a notable correlation between the MD signature, immune infiltration and
drug susceptibility. The expression levels of AGT, HCFC1, KIFC3, NOX4 and RIN1 mRNA
were all increased in these GC cells.
Evaluation of Anti-Proliferative and Apoptosis-Inducing Activities of the New Ciprofloxacin Derivative on Human Leukemia NB4 Cells
Authors: Goharkhany Selda , Eslami Farhad , Khalaj-Kondori Mohammad , Mahdavi Majid 
Published: October 2024

Backgrounds::
Leukemic stem cells are considered to be the main cause of treatment
failure and disease recurrence due to their resistance to most common therapies. Apoptosis induction
is one of the highly effective methods for treating cancer. Ciprofloxacin is among the compounds
whose antitumor effects have been confirmed.

Objectives:
In this study, we investigated the anti-proliferative effect and induction of apoptosis by
one of the derivatives of this family called 1-Cyclopropyl-6-fluoro-7-[4-(2-{[(1R,2S,5R)- 2-
isopropyl-5-methylcyclohexyl]oxy}-2-oxoethyl)-piperazin-1-yl]-4-oxo-1,4-dihydroquinoline-3-
carboxylic acid (ICH-CP) on NB4 cell line as an in vitro model of Acute promyelocytic leukemia
(APL). NB4 cells were treated using the ICH-CP combination in various concentrations.

Methods:
The viability of NB4 cells was evaluated by MTT assay, and their morphology of apoptosis
was examined by fluorescence microscopy. Flow cytometry and Annexin V/PI staining were
used to quantify apoptosis. Finally, the expression of three genes, Bax, Bcl-2, and Survivin was inquired
by real-time PCR.

Results:
According to the results, ICH-CP was able to destroy about 60% of NB4 cells in a dose
and time-dependent manner. Light microscopy and fluorescence microscopy studies on treated cells
confirmed the induction of apoptosis. Also, the real-time PCR analysis showed that ICH-CP induces
apoptosis in the NB4 cell line via the down-regulation of Survivin and Bcl-2, in contrast to the
up-regulation of the Bax gene.

Conclusion:
Based on the present data, it seems that the novel compound can be a good candidate
for the treatment of acute myeloid leukemia. Furthermore, it is recommended to evaluate the qualification
of ICH-CP as an adjunctive agent for other cancer cell lines.
Nano-reusable CuO-ZnO Catalyzed One-Pot Synthesis of 1-Amidoalkyl-2-Naphthols under Green Condition
Authors: Patil Gopinath G. , Muntode Bhausaheb B. , Jadhav Nitin K. , Dhere Vikram A. , Khabnadideh Soghra , Kale Raju R 
Published: October 2024

Introduction:
The Developed CuO-ZnO nanocomposite has been demonstrated as a new
and environmentally friendly catalyst for one-pot multicomponent reaction between aldehydes, 2-
naphthol and amides in the synthesis of 1-amidoalkyl-2-naphthols.

Method:
The new catalyst was synthesized by a ball-milling method by mixing a mixture of CuO
and ZnO powder in various proportions and characterized by different spectroscopic methods such
as powder X-ray diffraction (pXRD), Scanning Electron Microscopy (SEM), UV-Visible analysis,
EDAX elemental analysis and mapping. The advantages of the devised protocol include a green
approach, simple work-up procedures, avoidance of hazardous solvents, and good to excellent
yields. RE

Sult:
Evaluation of the catalyst performance in the synthesis of some 1-amidoalkyl-2-naphthols
showed presentable results. Sixteen derivatives were synthesized in desirable yield by our new
method (4a-4p). CuO-ZnO nanocomposite as a safe and efficient catalyst could be reused up to 5
runs for the synthesis of naphthol derivatives without any significant decrease in its potency.

Conclusion:
The High purity of the products and desirable yields are other points that make the
present work more attractive.
Enhancing the accreditation of Indonesian private universities through the integration of EduQual and accreditation standards of the BAN-PT
Authors: Pratiwi Mirandhi , Kusumah Lien Herliani 
Published: October 2024

Higher education institutions in Indonesia are currently facing significant challenges in maintaining public trust, requiring them to uphold autonomy, transparency, accountability, and continuously meet standards for quality assurance and improvement. This research aims to identify and address the unmet quality aspects in education to elevate the accreditation of private universities from C/Good to B/Very Good or even A/Excellent. By integrating the EduQual model with certification requirements from the Board of National Accreditation for Higher Education (Badan Akreditasi Nasional Perguruan Tinggi, BAN-PT), the research is supported by comprehensive studies of university accreditation reports, questionnaires, focus group discussions (FGD), and expert judgment through interviews and consultations. The data analysis employs gap analysis, importance-performance analysis, and quality function deployment. The study identified twenty-one priority improvement points to enhance accreditation, with six key improvements prioritized from the ninety indicators examined based on customer needs analysis. In the accreditation of private universities, the unmet aspects of education quality lie in the dimension of physical facilities, particularly human resources, as well as in the dimension of personal development, especially in the criteria for research and community service. The study recommends prioritizing investments in human resource development and strengthening research and community service initiatives, as these are critical areas where private universities fall short in meeting accreditation standards and fulfilling educational quality expectations.
E-Screening for Prenatal Depression in Kampala, Uganda Using the Edinburgh Postnatal Depression Scale: Survey Results
Authors: Namatovu Hasifah Kasujja , Magumba Mark Abraham , Akena Dickens 
Published: October 2024

Abstract

Background
Perinatal depression remains a substantial public health challenge, often overlooked or incorrectly diagnosed in numerous low-income nations.

Objective
The goal of this study was to establish statistical baselines for the prevalence of perinatal depression in Kampala and understand its relationship with key demographic variables.

Methods
We employed an Android-based implementation of the Edinburgh Postnatal Depression Scale (EPDS) to survey 12,913 women recruited from 7 government health facilities located in Kampala, Uganda. We used the standard EPDS cutoff, which classifies women with total scores above 13 as possibly depressed and those below 13 as not depressed. The χ2 test of independence was used to determine the most influential categorical variables. We further analyzed the most influential categorical variable using odds ratios. For continuous variables such as age and the weeks of gestation, we performed a simple correlation analysis.

Results
We found that 21.5% (2783/12,913, 95% CI 20.8%‐22.3%) were possibly depressed. Respondents’ relationship category was found to be the most influential variable (χ21=806.9, P<.001; Cramer’s V=0.25), indicating a small effect size. Among quantitative variables, we found a weak negative correlation between respondents’ age and the total EPDS score (r=−0.11, P<.001). Similarly, a weak negative correlation was also observed between the total EPDS score and the number of previous children of the respondent (r=−0.07, P<.001). Moreover, a weak positive correlation was noted between weeks of gestation and the total EPDS score (r=0.02, P=.05)

Conclusions
This study shows that demographic factors such as spousal employment category, age, and relationship status have an influence on the respondents’ EPDS scores. These variables may serve as proxies for latent factors such as financial stability and emotional support.
Stunting Super App as an Effort Toward Stunting Management in Indonesia: Delphi and Pilot Study
Authors: Erika Kadek Ayu , Fadilah Nur , Latif Aulia Insani , Hasbiah Nurhikmawaty , Juliaty Aidah , Achmad Harun , Bustamin Anugrayani 
Published: October 2024

Abstract

Background
Currently, 30 million children are experiencing acute malnutrition, and 8 million children are severely underweight.

Objective
This study aimed to develop a stunting super app, a one-stop app designed to prevent and manage stunting in Indonesia.

Methods
This study consisted of three stages. Stage 1 used a 3-round Delphi study involving 12 experts. In stage 2, 4 experts and a parent of children with stunted growth created an Android app containing stunting educational materials. In stage 3, a pilot study involving a control group was conducted to evaluate parents’ knowledge about stunting prevention through the app and standard interventions.

Results
In the Delphi study, 11 consensus statements were extracted; arranged in three major themes, including maternal health education, child health education, and environmental education; and applied in the form of the Sistem Evaluasi Kesehatan Anak Tumbuh Ideal (SEHATI) app. This app was assessed using a content validity index, with a cumulative agreement of ≥80% among the 5 individuals. The pilot study showed an increase in the knowledge of mothers of toddlers with stunted growth before and after the educational intervention (P=.001).

Conclusions
The SEHATI app provides educational content on stunting prevention that can increase the knowledge of mothers of toddlers with stunted growth.
Identifying Patient-Reported Care Experiences in Free-Text Survey Comments: Topic Modeling Study
Authors: Steele Brian , Fairie Paul , Kemp Kyle , D'Souza Adam G , Wilms Matthias , Santana Maria Jose 
Published: October 2024

Abstract

Background
Patient-reported experience surveys allow administrators, clinicians, and researchers to quantify and improve health care by receiving feedback directly from patients. Existing research has focused primarily on quantitative analysis of survey items, but these measures may collect optional free-text comments. These comments can provide insights for health systems but may not be analyzed due to limited resources and the complexity of traditional textual analysis. However, advances in machine learning–based natural language processing provide opportunities to learn from this traditionally underused data source.

Objective
This study aimed to apply natural language processing to model topics found in free-text comments of patient-reported experience surveys.

Methods
Consumer Assessment of Healthcare Providers and Systems–derived patient experience surveys were collected and linked to administrative inpatient records by the provincial health services organization responsible for inpatient care. Unsupervised topic modeling with automated labeling was performed with BERTopic. Sentiment analysis was performed to further assist in topic description.

Results
Between April 2016 and February 2020, 43.4% (43,522/100,272) adult patients and 46.9% (3501/7464) pediatric caregivers included free-text responses on completed patient experience surveys. Topic models identified 86 topics among adult survey responses and 35 topics among pediatric responses that included elements of care not currently surveyed by existing questionnaires. Frequent topics were generally positive.

Conclusions
We found that with limited tuning, BERTopic identified care experience topics with interpretable automated labeling. Results are discussed in the context of person-centered care, patient safety, and health care quality improvement. Furthermore, we note the opportunity for the identification of temporal and site-specific trends as a method to identify patient care and safety concerns. As the use of patient experience measurement increases in health care, we discuss how machine learning can be leveraged to provide additional insight on patient experiences.
The Path of Breaking Out of the Circle for Prose Works in Film and Television as Seen from To the Wonder
Authors: Xiong Qiongyu 
Published: October 2024

The TV series To the Wonder, adapted from a prose work, has garnered phenomenal attention with its high-quality content and poetic and healing style. The drama has achieved success in script adaptation by creating quality text content, and it has provided audiences with fresh aesthetic experiences by using visual language and poetic and scattered cultural narrative. At the same time, the content's dissemination and marketing are based on a multi-media matrix, with multi-channel dissemination, and it has explored a new path for diverse expression of prose works through the creation of IP-based derivative products in the film and television industry.
Role of Inflammation and Motility Disorders in the Development, Course and Consequences of Functional Gastrointestinal and Biliary Tract Diseases (Literature Review and Expert Panel Resolution)
Authors: Ivashkin V. T. , Bordin D. S. , Lapina T. L. , Livzan M. A. , Okhlobystin A. V. , Poluektova E. A. , Trukhmanov A. S. , Uspenskiy Yu. P. , Shulpekova Yu. O. 
Published: October 2024

Aim: to present the results of the Expert Panel with a discussion of modern concepts of the pathogenesis of functional gastrointestinal diseases and the possibilities of multitarget therapy with trimebutine. Key points. Low-grade inflammation can be considered as a morphological substrate of functional diseases with an increase in activated mastocytes and eosinophils, T-helpers 2 and T-helpers 17 in the gastrointestinal mucosa. In the development in the content of visceral hypersensitivity, the functional connection between mastocytes and TRPV1-positive sensory endings of the vagus nerve is of great importance. Proinflammatory cytokines and matrix metalloproteinases can enter the systemic circulation, provoking the development of systemic manifestations. Increased levels of proinflammatory cytokines are supported by altered intestinal permeability and microbiota. Functional diseases are believed to modify the symptoms and course of concomitant organic diseases of the gastrointestinal tract (for example, functional diseases of the biliary tract may contribute to the development of cholelithiasis, pancreatitis). The peripheral μ-, κ- and δ-receptor agonist trimebutine (Trimedat®) regulates the production of enterohormones, modulates motility throughout the gastrointestinal tract and normalizes visceral sensitivity. The effectiveness of trimebutine in the treatment of functional disorders has been shown in various studies. Trimebutine helps reduce the production of proinflammatory cytokines, including interleukin-6. Conclusion. In the treatment of functional diseases of the gastrointestinal tract, trimebutine can be considered as a multitarget agent, since the drug helps to normalize motility, reduces the degree of visceral hypersensitivity, exhibits anti-inflammatory and neuroregenerative effects, and can also increase the effectiveness of treatment of concomitant diseases.
Esophagoplasty Using a Jejunum Segment on a Vascular Pedicle
Authors: Ruchkin D. V. , Kozlov V. A. , Khamidov M. M. , Okonskaya D. E. , Kovalerova N. B. 
Published: October 2024

Aim: to substantiate the expediency of using a segment of the jejunum on a vascular pedicle in esophagoplasty. Materials and methods. At the National Medical Research Center of Surgery named after A.V. Vishnevsky, 12 patients underwent esophagoplasty with a combined visceral transplant, of which 9 (75.0 %) received a gastrointestinal graft and 3 (25.0 %) — a colon-jejunum one. Esophagectomy with simultaneous esophageal plastic surgery was performed in 10 (83.3 %) patients, in 4 (40 %) of them — in combination with distal gastric resection. The indication for surgery in 3 patients was cicatricial stricture of the esophagus and stenosis of the pylorus, in 2 — achalasia of the cardia of the terminal stage (in one patient — with ulcerative stenosis of the pylorus). Three more patients underwent esophagectomy due to peptic strictures: esophagogastroanastomosis (n = 1), esophagoenteroanastomosis (n = 1) and esophagus after Nissen surgery (n = 1). Cancer recurrence after proximal gastric resection and gastrectomy in 2 patients was an indication for esophagectomy. Also, 2 (16.7 %) patients were hospitalized in the National Medical Research Center of Surgery named after A.V. Vishnevsky with incomplete esophagoplasty: one person — after Dobromyslov — Torek surgery for spontaneous rupture of the esophagus, and one patient — after the unsuccessful Lewis surgery for esophageal cancer performed in other hospitals. Results. The average duration of post-operative stay was 12.7 ± 6.3 days. The postoperative period was smooth in 10 (83.3 %) patients. The failure of the cervical anastomosis was revealed in 1 (8.3 %) patient on day 5 after esophageal plastic surgery with colon-jejunum graft, which was resolved conservatively without repeated surgery. Relaparotomy was required in one patient on day 7 after surgery for bile peritonitis, the cause of which was destructive acalculous cholecystitis with perforation. All 12 patients were discharged from the clinic with full nutrition through the mouth. In the long-term period, 11 (91.7 %) patients remained under observation, 1 (9.1 %) developed an esophagogastroanastomosis stricture 1.5 months after esophageal plastic surgery, which was resolved by 4 courses of bougation during the first 9 months after surgery. No other complications were noted. Conclusion. The segment of the jejunum on the vascular pedicle, used for esophagoplasty, in the condition of a shortage of plastic material, allows not only to complete the reconstruction at once, but also to restore the natural passage of food and prevent bile reflux.
The Role of Macrophages in the Pathogenesis of Celiac Disease
Authors: Khavkin A. I. , Loshkova E. V. , Kondratieva E. I. , Shapovalova N. S. , Grishkevich I. R. , Doroshenko I. V. , Yankina G. N. 
Published: October 2024

Aim: to present data on the involvement of macrophages in the pathogenesis of celiac disease and the development of possible treatment methods for this disease aimed at changing the function of macrophages.Key points. Celiac disease is an autoimmune disease with a characteristic serological (antibodies to tissue transglutaminase, endomysium, deamidated gliadin peptides) and histological profile (inflammatory infiltration of the villous epithelium by lymphocytes and their atrophy, crypt hyperplasia) caused by gluten consumption in genetically predisposed individuals. Macrophages, as key cells that provide a link between innate and adaptive immunity, are of significant importance in the pathogenesis of celiac disease. Gliadin peptides stimulate the activation of macrophages according to the proinflammatory phenotype with the production of cytokines, which causes the immune response of T-helpers 1 and T-helpers 17. The result of these processes is the development of an inflammatory reaction and damage to the intestinal mucosa due to the production of matrix metalloproteinases and reactive oxygen species by macrophages. Therapeutic tactics for celiac disease today include a gluten-free diet, which is not so easy to follow. Of interest is the study of the possibility of using polyphenols in celiac disease, which are capable of precipitating gliadins and inhibiting the polarization of macrophages towards a proinflammatory phenotype, while simultaneously stimulating an increase in the population of macrophages of an anti-inflammatory phenotype associated with a decrease in tissue damage.Conclusion. Impaired macrophage function/differentiation results in either inadequate, excessive immune activation or failure to mount effective protective immune responses against pathogens, which may result in the development of gastrointestinal diseases. Studying the involvement of macrophages at different stages of celiac disease progression is important for the development of new treatments for this disease.
A comparison of oral health-related quality of life and satisfaction among patients undergoing root canal treatment or tooth extraction – A prospective controlled cohort study
Authors: Wigsten Emma , Camci Emine , Levinsson Anna , Kvist Thomas , Sebring Dan , EndoReCo  , Davidson Thomas 
Published: October 2024

Objective: This study aimed to evaluate the impact of root canal treatment on Oral Health-Related Quality of Life (OHRQoL) in general dental practice and compare it with tooth extraction. Additionally, patient satisfaction following tooth-preserving treatment was assessed.
Material and methods: In all, 65 patients were recruited from 6 general dental clinics in Västra Götaland over 8 weeks, with 37 starting root canal treatment and 28 having extractions. Questionnaires, including Oral Health Impact Profile-14 (OHIP-14) and 9 questions assessing patient satisfaction, were administered at treatment initiation, and at 1, 6, and 12 months. The responses from both modalities were analysed using descriptive and analytical statistical methods.
Results: The response rate ranged from 73.8% to 92.3%. Regarding OHRQoL, differences between the groups were few compared to baseline. However, significant improvements were observed in the extraction group at the 6- and 12-month follow-ups, in the ‘total score’, and the dimensions ‘pain’, ‘discomfort’, and ‘handicap’. Patient satisfaction was generally high, with cost being the least satisfactory item. Pain intensity remained consistently low.
Conclusions: In this prospective cohort study few differences were found between the two treatment modalities. However, significant improvements were observed in the extraction group in several dimensions. The patient satisfaction regarding root canal treatment was considered high.