bluuebunny/crossref_metadata_2025_split huggingface dataset:
Показники клітинного метаболізму в сироватці крові коней за латентного перебігу лептоспірозу та ринопневмонії
Authors: Halatiuk O. , Kalnaus R. , Rublenko M. , Yeroshenko O. 
Published: November 2017

The article presents the results of research on the parameters of cellular metabolism in 141 horses. Studies were conducted in one of the unsuccessful with leptospirosis equine farm. The kits of the company «Reagent» (Dnipropetrovsk) in blood serum determined the content of such acute phase proteins as ceruloplasmin by the method of Ravin, haptoglobin by reaction with rivanolol, total protein by biuretovoy reaction, albumin by reaction with bromocresol green by methods K.M. Veremeenko et al. (1988). The concentration of nitrites was determined by the interaction of plasma nitrides with the Grice reagent, and the colored complex was colorimetricized using a spectrophotometer at a wavelength of 540 nm using the method of Grand F. et. al. (2001) in the modification of Golikov P.P. (2004). The production and recording of PMA and lysis was carried out in accordance with the «Manual on Laboratory Diagnosis of Leptospirosis» (1997). The reaction of the RDP for the diagnosis of rhinopneumonia of horses was carried out in accordance with the methodological recommendations (2009). The first group (control) was represented by 49 clinically healthy serologically negative in the reaction of microagglutination (PMA) and diffusion precipitation (RDP) reactions with respect to leptospirosis and rhinopneumonia. The second group with latent course of leptospirosis included 51 horses. The third group with latent course of leptospirosis and rhinopneumonia included 41 horses. As a result of our research, we established a significant increase (P < 0.05–0.01) of nitric oxide to 65.73 ± 4.43 and 55.86 ± 2.71 μmol/l in 2–3 groups in comparison with healthy. There is also a significant increase in ceruloplasmin (P < 0.01–0.001) to 216.35 ± 11.43, 243.15 ± 19.34 mg/l in 2–3 groups in comparison with a healthy one. The content of hapthoglobin, on the contrary, decreases to 0.61 ± 0.03 and 0.52 ± 0.04 * g / l in 2–3 groups compared with healthy. Albumin content did not differ significantly in the studied groups. At the same time, a significant reduction of fibrinogen (P < 0.01–0.001) was established to 1.23 ± 0.09 and 1.22 ± 0.08 g/l in 2–3 groups in comparison with healthy ones. The content of soluble fibrin (P < 0.001) to 16.05 ± 0.31 and 21.22 ± 0.71 mg/% in 2–3 groups in comparison with healthy is significantly increased. In a coherent latent course of leptospirosis and rhinopneumonia in horses as markers for assessing the state of immunobiological reactivity of an organism it is advisable to determine the content of nitric oxide, ceruloplasmin, soluble fibrin, fibrinogen and haptoglobin in blood serum. The results obtained are analyzed for the expediency of conducting a course of intensive care for especially valuable horses.
Таксономічна характеристика РНК-геномних вірусів хребетних тварин і людини
Authors: Kalinina O.S. 
Published: November 2017

The article presents a modern taxonomy and nomenclature of viruses of vertebrates animals and human based on information ICTV release 2016 (ratification 2017). Described the basic criteria for the classification of viruses: characteristics of the viral genome, the mechanism of replication and virions structure. Viruses of vertebrates (1269 species) consist of 5 orders, 38 families, including 12 – DNA-genomic and 26 – RNA-genomic, 12 subfamilies and 233 genera. RNA-genomic viruses of vertebrates (679 species) classified of 4 orders, 26 families, 6 subfamilies and 119 genera. The order Mononegavirales has united family Paramyxoviridae, Pneumoviridae, Rhabdoviridae, Filoviridae, Bornaviridae, Nyamiviridae and Sunviridae, order Nidovirales – family Coronaviridae and Arteriviridae, order Bunyavirales –family Hantaviridae, Nairoviridae, Peribunyaviridae and Phenuiviridae, order Picornavirales – family Picornaviridae. Family Rhabdoviridae, Nodaviridae, Peribunyaviridae, Phenuiviridae, Reoviridae and Birnaviridae, except viruses of vertebrates, contain viruses of insects, and family Rhabdoviridae, Phenuiviridae and Reoviridae – viruses of plants. There is а one of «floating» genus Deltavirus, which is not included of families. The family Reoviridae includes the Eriocheir sinensis reovirus, and the family Birnaviridae – Tellina virus. Described the taxa of viruses: family, subfamily, genera, species. Named typical species genera of viruses. Characterized the basic taxonomic features of RNA-genomic vertebrates viruses of animals and human: the shape, size and structure of virions – the presence of outer membrane lipoprotein, capsid symmetry type (spiral, icosahedral), the structure of the viral RNA (the number of threads, conformation, fragmentation, polarity). The attention to virus reproduction features. Replication of most RNA-genomic viruses occurs in cells of the cytoplasm, except for the representatives of the families Bornaviridae, Nyamiviridae, Orthomyxoviridae, Retroviridae and «floating» genus Deltavirus, which are replicated in the nucleus. Output of the progeny virions in simply organized viruses is due to cell destruction, and in most of the complexly organized viruses – plasma membrane buds, as well as through the membranes of the Golgi complex or the endoplasmic net in combination with exocytosis (Peribunyaviridae, Hantaviridae, Nairoviridae, Phenuiviridae, Flaviviridae, Coronaviridae, Arteriviridae).
Вплив трансплантації культур клітин на перебіг експериментального цукрового діабету у тварин
Authors: Kovpak V.V. 
Published: November 2017

As we know from the literature data, type 1 diabetes mellitus occurs due to the loss of β-cells of island of Langerhans, manufacturing insulin, by the organism, which causes its deficiency. The treatment of this type of diabetes, even at early stages, lies in the substitution therapy together with a careful control of blood glucose which may last for life. It is for this reason that the cellular technologies as an alternative method of treatment of this pathology are growing more and more relevant.Objective of the study: to study the impact of allogenic transplantation of the cultures of bone marrow, lipid tissue and pancreas cells on the course of experimentally generated diabetes mellitus in animals.Task: 1. To obtain the cultures of bone marrow, lipid tissue and pancreas cells in rats. 2. To compare the impact of allogenic transplantation of the cultures of bone marrow, lipid tissue and pancreas cells in the course of experimentally generated diabetes mellitus in rats. 3. To study the impact of pancreas cells culture on the course of experimental diabetes mellitus in cats.The studies were conducted using clinically healthy animals (30 males of white non-pedigree rats with body weight of 200–250 g, aged 4–5 months; 9 white non-pedigree junior rats aged 12 days; 4 mongrel cats aged 15–17 months) and missed fetuses of cats remained after obstetric aid.Alloxan diabetes was generated by means of single subcutaneous injection of alloxane monohydrate in the dose of 150 mg/kg in the form of 5% solution on citrate buffer (pH 4.5) after preliminary 24-hour absolute diet with free access to water.The cultures of bone marrow and pancreas cells were obtained from the bone marrow of tubular bones and pancreas of puppies aged 12 days correspondingly, lipid tissue – from rats aged 4–5 months. The culture of cats’ pancreas cells was obtained from the pancreas of cat fetuses. Cell culture process was carried out according the standard method in CO2- incubator. Glucose level in blood serum was determined by means of electrochemical analysis.The results of the study. The authors studied the impact of allogenic transplantation cells culture of bone marrow, lipid tissue and pancreas on the course of experimentally generated diabetes mellitus in rats. The study revealed the decrease of glucose level in the blood of the animals under investigation at cell material transplantation. The most efficient culture for experimental diabetes mellitus therapy is the culture of pancreas cells which has become the cause of its further study in cats.During allogenic transplantation cells culture of pancreas in cats the authors observed abrupt decrease of glucose level in the blood of the animals under investigation immediately after cell transplantation with the further approximation to the initial state.The experimental models presented the positive effect of cell culture transplantation providing the grounds for the further implementation of this diabetes mellitus therapy method in the clinical practice.
Ідентифікація небезпечних чинників під час вирощування райдужної форелі в умовах замкнутого водопостачання
Authors: Grynevych N. , Dyman T. , Kukhtyn M. , Semanyuk V. , Sliusarenko A. 
Published: November 2017

Identification of dangerous factors for a conditional industrial farm for rainbow trout growing in conditions of Recurculating aquaculture system (RAS) was carried out. During the analysis of dangerous factors, the degree of significant influence and probability of potential dangers were determined. Significant potential hazards have been identified at such technological stages as insemination and incubation of caviar, larval survival, caviar, larvae, young, commodity fish and pedigree.Biological filter is defined as a key link in the RAS the functioning of which effects on the quality and safety of commercial fish. It carries out aerobic and anaerobic processes to remove contaminations in the form of ammonium producing by fish, and carbon dioxide, which is formed from undigested food and feces. There are such dangerous factors on the stage of biological purification of water: biological – possible exceeding the total microbial number (more than 100 CFU/cm3), the number of nitrifying (more than 106 CFU/cm3) and E. coli groupbacteria, the presence of bacteria Pseudomonas aeruginosa; chemical – exceeding the content of nitrates (more than 50 mg / dm3), nitrites (more than 0.5 mg/dm3), pH – above 8.In addition to purely technological, there are additional dangerous factors – related to the functioning of the biofilter, fish feeding and the use of veterinary preparations and disinfectants. The potential hazards identified in the work are important to be taken into account in the process of implementation of Rainbow Trout HACCP plan.
Aлгоритм імплементації належної практики молочного фермерства з метою отримання безпечного та якісного молока-сировини
Authors: Kondrasii L.A. , Iakubchak O.M. , Shevchenko L.V. 
Published: November 2017

Currently, Ukraine has not control of sanitation and hygiene of obtaining raw milk on the farm. This is connected of the Order of the Prime Minister of Ukraine dated January 20, 2016, No. 94-р «On the recognition of invalid acts, and those that are not applicable in Ukraine, regulations of sanitary legislation» The assessment of some safety and quality indicators of milk is carried out only when milk is received for processing. An algorithm for the implementation of good dairy farming practices in Ukrainian farms has been developed, that give consideration the analysis of the current practices of raw milk production in Ukraine. The algorithm is based on the principles of good dairy farming practices and good farming practices for animal production food safety. The implementation of the algorithm will give an opportunity to provide and analyze of raw milk safety and quality parameters on Ukrainian farms. An algorithm of good dairy farming practices implementation on Ukraine farms take into account the creation of a working group, the development of 12 programs (which are provided with the 71st instruction) and the establish a farmstead laboratory.Give consideration research data about technical support and milk production practices at Ukrainian farms, we have been developed two blocks of programs to implement good dairy farming practices. a block of cow welfare programs and a block of programs for obtaining safe and high-quality milk-raw materials. The first – A block of programs for ensuring of cows welfare, includes programs: good stable climate, dairy barn and farm territory hygiene and technical condition, feed and water hygiene, good veterinary management, calves management, and occupational health of cow's carers. The second – A block of programs for ensuring safety and quality raw milk, includes programs: preparation of cows for milking, good milking practice, post-milking measures, control of support for technical serviceability of dairy equipment, good sanitary and hygienic status of dairy equipment and milking room, and the hygiene and occupational health of persons employed in the milking of the farm.
Ветеринарно-санітарна оцінка якості та безпеки харчових продуктів у Житомирському регіоні
Authors: Kotelevich V.A. 
Published: November 2017

The results of Veterinary Expertise of food according to Zhytomyr Regional State Veterinary Laboratory (2014–2015rr.) And state laboratories Veterinary Expertise of household markets. Zhytomyr and Zhytomyr region. The main reason for culling offal 2014–2015 gg. Were invasive disease, including 658 (3.45%) patients in the study Fasciolosis products of slaughter cattle, 5033 – echinococcosis (3.59%) and 413 (0.29%) – Metastrongillosis in pigs. In terms of safety and quality (content of toxic elements, pesticides, mycotoxins, antibiotics) sausage and cooked sausage highest, grade 1 and 2 met the regulatory requirements. For sanitary indicators in 4.4% of samples of meat products were isolated Escherichia coli in 11.1% – mesophilic aerobic and facultative anaerobic microorganisms. They can, under appropriate conditions, cause food poisoning, so sausage wares have been directed to disinfection by evaporation and translated into lower grades. We conducted bacteriological tests of meat samples availability of enterobacteria, MAFAnM (mesophilic aerobic and facultative anaerobic bacteria) and bacteria of the genus Salmonella immediately after slaughter showed that the number of microorganisms on the surface of the ink was: kind of Enterobacteriaceae 28,5 ± 1,1 × 105 CFU/cm2, mesophilic aerobic and optionally anaerobic microorganisms (MAPANM) – 38.8 ± 1.05 CFU × 103/cm2. Salmonella from carcass was not detected. The dairy product safety guarantor in Ukraine remains a system for monitoring sanitary hazards and residual amounts of toxic substances. In order to eliminate the risk of dangers to the consumer of dairy products, it is necessary to improve the control system of raw materials used for manufacturing products, according to safety indicators at all stages of production. Therefore, we consider it appropriate to draw the attention of specialists of veterinary medicine in the veterinary and sanitary conditions of milk and dairy products, gain control in government laboratories vetsanekspertizy household markets using antibiotics and in slaughtered animals to prevent a realization of substandard products, improve the sanitary condition of the storage and implementation of animal Products, prevention of invasive and non-contagious diseases in cattle and pigs.
Бактерицидні та дезінфікуючі властивості деззасобу «Арквадез-плюс»
Authors: Tishyn O.L. , Kopijchuk G.T. , Khomiak R.V. , Khyrivskyy O.V. , Orynchak Т.V. 
Published: November 2017

The results of research bactericidal properties of new domestic disinfectant «Arquadez–plus» which is a transparent light color, without mechanical inclusions, solution with a specific odor, and created on the basis of quaternary ammonium compounds (QAC), in particular, the composition of the disinfectant include: alkyldimethylbenzylammonium chloride and dimethyldipetylammonium chloride. Was found bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (PC) and protein index (PI). Most susceptible to the effect of disinfectant was gram-negativ bacteria of E. coli, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.0129 and 0.0092%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, cell death came at these exposures at concentrations of 0.0252 and 0.018%, respectively. Microorganisms P. vulgaris were the least susceptible to the action of the disinfectant; the death of the microorganisms came at concentrations of 1,0204 and 0.5206%, for 10 and 30 minute exposures, respectively, and, due to the action of the disinfectant on the test culture of S. typhimurium, the death of cells came under given conditions, at concentrations of 0.5206 and 0.2656%, respectively. The action of disinfection preparation for vegetative cells of B. subtilis cell death came on the 10 and 30 minutes exposures at concentrations of 0.0494 and 0.018% but in the spore form, at concentrations of 0.5206 and 0.3719%, respectively. The disinfectant effect of this disinfectant is 79.4 and 28.9 times higher on microorganisms E. coli and S. aureus, respectively, of the decontaminant effect of phenol, and in the presence of a protein, the activity of the disinfectant is reduced by 1.4 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test-objects.It is established that test-cultures of E. coli, S. aureus and P. vulgaris at 0.05% concentration of disinfectant is ineffective and for the test culture of S. typhimurium, its 0.1% concentration is ineffective.For vegetative and spore form of B. subtilis disinfection preparation concentration at 1.0%. Is ineffective at the 0.5–1.0% concentrations is effective for processing rigid surfaces, at preventive disinfection of premises for animals and birds at exposure for 60 minutes, and at spore forms of microorganisms its working concentration should be 2.0%, at exposure in 120 minutes and 3.0% at exposures in 30 minutes or more. Proved that the disinfectant «Arquadez-plus» in a production environment at a concentration of 1.0% by wet surface irrigation facilities for animals and working solution 0.25–0.3 liters per 1 square meter with an exposure of 2 hours, showing high disinfectant properties.
Вплив хелатних сполук (метіонатів) на м’ясні якості та ветеринарно-санітарні показники яловичини
Authors: Farionik T.V. , Gnatyuk V.V. 
Published: November 2017

Providing the population with food and, first of all, meat is one of the main prerequisites for the development of Ukraine. Biologically complete and relatively inexpensive beef can only be obtained from animals of the meat production direction. One of the conditions for obtaining high-quality products is the full feeding of animals, which allows them to realize the genetic potential laid in the breed. At the same time, the problem of balancing rations for a microelement nutrition, which is characterized by a shortage in soil and feeds of macro- and microelements, becomes important. Some of these elements are components of biologically active compounds and regulators of various metabolic processes, and their lack or excess can lead to significant metabolic disorders in the animal's body and loss of productivity.Despite numerous studies devoted to the study of metabolism and individual parts of antioxidant protection of animals, work revealing the physiological and biochemical mechanisms of these processes in cattle for the influence of scarce microelements is quite limited. The issues not only of protein metabolism and the antioxidant defense system in the age aspect remain, but also the course of these processes for the correction of mineral nutrition, which predetermined the choice of the direction of research, the purpose and objectives of this work.The imbalance in the diet of cattle fattening on mineral substances is accompanied by a violation of metabolism, reduced productivity and resistance of animals, deterioration of the indicators of veterinary and sanitary examination and meat quality. Ratios of cattle, which include feeds require the mandatory enrichment of their iron. Feeding iron metionates positively affects the physiological state of the organism, increases the number of red blood cells, hemoglobin.When using iron in the form of chelates, a high coefficient of its assimilation is established. In blood, the serum iron content of iron increased and this contributed to a decrease in total and latent iron binding capacity of serum. Iron chelates (metionates) more intensively stimulate protein synthesizing function, which manifests itself by increasing the content of total protein, albumin and reducing the amount of globulins.Application of micronutrients and their chelate compounds (metionates) and other biologically active substances has its advantages, the level of assimilation of heavy metals, radionuclides from contaminated feeds and water is reduced, chelate complexes of trace elements (МЕ) easily penetrate through cell membranes, allowing to carry out purposeful influence on exchange substances and energy and to correct the deficit of ME in the relevant biogeochemical zones.
Вплив препарату Максідін на загальну резистентність організму собак розплідникового утримування
Authors: Sanin A.V. , Annikov V.V. , Narovlyansky A.N. , Pronin A.V. , Mezentseva M.V. 
Published: November 2017

In the conditions of dense keeping dogs are constantly faced with the attack of the representatives of conventional and conventionally pathogenic microflora, which ultimately leads to a decrease in resistance of animals. Thus, in the case of appearance of new animals in a kennel, or after visiting exhibitions and competitions it’s appropriate to use harmless and effective drugs to increase natural resistance of the organism. The aim of this work was to assess the effectiveness of Maxidin 0.4 in improving the natural resistance of dogs kept in the kennel. The work was carried out on 20 dogs which were divided into 2 equal groups. Dogs in the control group received saline at a dose of 1 ml. Dogs of the experimental group were inoculated with Maxidin 0.4 at a dose of 1 ml, subcutaneously. Clinically at the start of the study we noted the reduction of appetite, slight serous-catarrhal discharge from conjunctiva of eye and nasal cavity, ruffled hair. 5 days after application of Maxidin 0.4 dogs of the experimental group showed improvement of appetite, weakening or absence of the serous and serous-catarrhal discharge from the conjunctiva and nasal cavity, skin turgor increased, the animals become more active. At the start of the study blood analysis showed signs of anemia, erythropenia, slight leukopenia and increase in hematocrit. 5 days after administration of Maxidin 0.4 we observed disappearance of anemia, erythropenia and leukopenia, normalization of hematocrit. Serum levels of IFN-α and IFN-γ increased 3–6 times.Thus, Maxidin 0.4, which contains germanium in an easily digestible form improves the clinical state and normalizes blood counts in dogs kept in the kennel. Also Maxidin 0.4 was shown to increase the levels of IFN-α and IFN-γ in the blood serum of dogs, which is very important as in previous studies this effect was found only in experiments with laboratory animals.