bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of magnesium levels in pregnancy on adverse birth outcomes: a protocol for a systematic review and meta-analysis
Authors: Abdul Naeera , Dorsamy Vinogrin , Bagwandeen Chauntelle 
Published: October 2024

Abstract

Background:
Magnesium, an often-overlooked trace element, may play a pivotal role in maternal and fetal health outcomes during pregnancy. Magnesium deficiency has been observed to contribute to several maternal complications, such as intrauterine growth restriction, pregnancy-induced hypertension and pre-eclampsia. Pre-eclampsia, a hypertensive disorder of pregnancy, presents substantial risks to both maternal and fetal health. Magnesium sulphate is a cornerstone in the management of pre-eclampsia, underscoring the importance of magnesium in pregnancy. Notably, pre-eclampsia is more prevalent in low-to-middle-income countries, where other co-morbidities such as HIV, anaemia and obesity are also common. Understanding the dynamics of magnesium levels in pregnancy and their interactions with co-morbidities could be critical for improving maternal and fetal health outcomes worldwide.
Method:
This protocol is for a systematic review and meta-analysis that aims to comprehensively map the literature on magnesium levels in pregnancy. Following the Preferred Reporting Items for Systematic Review and Meta-analysis for Protocols guidelines, our study will provide a rigorous synthesis of available evidence. By conducting a thorough search across multiple databases, including PubMed, Scopus, Directory of Open Access Journals, and Google Scholar, we aim to identify studies reporting on magnesium levels during pregnancy. Through screening and data extraction, we will analyze the association between magnesium levels and pregnancy complications, with further exploration of the impact of co-morbidities on magnesium status in pregnancy.
Discussion:
The findings of this study may have the potential to affect maternal and fetal health outcomes globally. Improved understanding of magnesium's role in pregnancy physiology can guide targeted interventions aimed at reducing the burden of pre-eclampsia and its associated complications, particularly in low-to-middle-income countries where maternal and infant mortality rates remain unacceptably high.
Prospero Registration: CRD42024518427
The consortium project INTEGRATE-ADHD - Comparison and integration of administrative and epidemiological ADHD diagnostic data by clinical assessment: study description and sample characteristics
Authors: Beyer Ann-Kristin , Beck Lilian , Pfeifer Stefan , Kuhnert Ronny , Hölling Heike , Jans Thomas , Berner Annalena , Hetzke Leila , Weyrich Sophia , Scholz Vanessa , Emser Theresa , Mager Diana , Ulsamer Sanna , Wallau Chantal , Romanos Marcel , Gilbert Martha , Kaman Anne , Ravens-Sieberer Ulrike , Witte Julian , Hasemann Lena , Weinert Katharina , Greiner Wolfgang , Widmann Jonas , Grau Anna , Horn Anna , Fiessler Cornelia , Heuschmann Peter , Riederer Cordula , Schlack Robert 
Published: October 2024

Abstract

Background
The consortium project INTEGRATE-ADHD examines differences between administrative and epidemiologically parent-reported Attention-deficit/hyperactivity disorder (ADHD) diagnostic data of children and adolescents in Germany. Therefore, parents of youths with an administrative ADHD diagnoses were surveyed using the questionnaires of the German Health Interview and Examination Survey for Children and Adolescents (KiGGS) and a subsample was clinically examined.
Methods
Parents of children and adolescents insured with the third largest German statutory health insurance DAK-Gesundheit with at least one confirmed administrative ADHD diagnosis in one quarter of the insurance year 2020 were surveyed online. The epidemiological questionnaire contained information on ADHD diagnosis, disorder-specific and comorbid psychopathology, health care utilisation, quality and satisfaction with care, risk and protective factors, and health-related quality of life. A subsample participated in a guideline-based clinical online assessment. Administrative, epidemiological and clinical data were linked on person-level.
Results
A total of n = 5,461 parents of children and adolescents with an administratively documented ADHD diagnosis (mean age = 12.5 years, 25.4% girls) responded to the online questionnaire (response rate: 21.5%). A representative subsample consisting of 202 families participated in the clinical online assessment. In 201 children and adolescents, it was possible to determine whether ADHD was clinically present.
Conclusion
Combining administrative, epidemiological and clinical ADHD diagnostic data on person-level, the consortium project INTEGRATE-ADHD offers an innovative and unique data-linkage approach that will provide a better understanding of the meaning and validity of ADHD prevalence data originating from various data sources. In addition, information on treatment satisfaction in affected child and adolescents and their families and potential deficits of health care in the context of ADHD as well as disorder-related direct health care costs may be derived.
Transcriptional reprogramming in oral squamous carcinoma
Authors: Cheng Xianyang , Shen Shan 
Published: October 2024

Abstract

Oral squamous cell carcinoma (OSCC) is a prevalent form of cancer globally, originating from a diverse range of neoplastic transformations in the oral mucosa. This condition is characterized by its complex genetic underpinnings, involving the intricate regulation of multiple genes. Genetic factors influence cellular processes such as growth, differentiation, and apoptosis of oral mucosal cells, thereby promoting or inhibiting tumor formation and progression. Furthermore, environmental factors—including smoking, alcohol consumption, and human papillomavirus (HPV) infection—can significantly increase the risk of developing OSCC. These external influences can impact the disease in several ways. Delayed clinical detection and the absence of specific biomarkers, coupled with expensive treatment alternatives, contribute to poor prognoses among OSCC patients. Thus, identifying OSCC biomarkers has become imperative. This study investigates publicly accessible sequencing data of oral mucosal tissues from four distinct datasets—GSE23558, GSE30784, GSE36090, and GSE51010—archived in the Gene Expression Omnibus (GEO) database. By analyzing these datasets, which encompass a range of genetic profiles and experimental conditions, the study seeks to uncover critical biomarkers and molecular pathways involved in the early stages of OSCC development. The primary objective is to identify pivotal genes linked to the onset of OSCC. The findings provide preliminary evidence for therapeutic targets in OSCC and may serve as a robust foundation for subsequent biological research endeavors.
Metabolomic and Microbiota Profiles in Cervicovaginal Lavage Fluid of women with High- Risk Human Papillomavirus Infection
Authors: Shen Su , Zhao Shixian , Shan Jinjun , Ren Qingling 
Published: October 2024

Abstract

The presence of high-risk human papillomavirus (HR-HPV) contributes to the development of cervical lesions and cervical cancer. Recent studies suggest that an imbalance in the cervicovaginal microbiota might be a factor in the persistence of HR-HPV infections. In this study, we collected 156 cervicovaginal fluid(CVF) of women with HR-HPV infection, which were divided into three groups(negative for intraepithelial lesions = 78, low/high-grade squamous intraepithelial lesions = 52/26). We performed metabolomics and 16S rRNA sequencing to identify changes in metabolites and cervicovaginal microbiota among patients with HR-HPV infection and varying grades of cervical lesions. We detected 164 metabolites and 389 flora types in the three groups. Ten CVF metabolites—N-methylalanine, phenylacetaldehyde, succinic acid, 2-3-dihydroxypyridine, DL-p-hydroxylphenyllactic acid, gluconic acid lactone, guanine, glucose-6-phosphate, erythrose, and sucrose showed significant associations with disease severity (P < 0.05) and distinct separation patterns in HR-HPV-infected patients with LSIL and HSIL, with an area under the curve of 0.928. The most abundant microbial communities in the CVF were Lactobacillus and Gardnerella. There was a significant negative correlation between succinic acid and Gardnerella. These findings suggest significant systemic metabolic changes in HR-HPV infection as it progresses to cervical lesions, providing valuable insights into the associated metabolic alterations and their association with disease severity.
A Nomogram Predicting 1-Year Co-Progression of Carotid and Coronary Plaque in Patients After Coronary Stenting
Authors: Zhang Chunyan , Li Min , Zhang Chi , Sun Yanyi , Chen Zhenyue 
Published: October 2024

Abstract

Backgroud: The concordance of carotid and coronary plaque progression warrants investigation.
Objectives: We aimed to analyze the correlation between them and to explore a nomogram for predicting multisite plaque co-progression in patients implanted with coronary drug-eluting stents (DES).
Methods: 909 patients were enrolled and randomized in a 7:3 ratio to the training and validation groups. LASSO (Least absolute shrinkage and selection operator) and logistic regressions determined risk factors. The nomogram visualized the prediction model. Concordance index (C-index), receiver operating characteristic (ROC) curve, and calibration curve validated the discrimination and calibration of the model. Decision curve analysis (DCA) and clinical impact curve assessed the clinical utility value of the nomogram.
Results: In our study, there was no statistical correlation between the concordance assessed by McNemar's test. Five variables were selected to establish the nomogram which displayed a robust discriminative ability with C-index of 0.837 [95% confidence interval (CI)=0.779-0.895) and 0.802 (95% CI=0.762-0.842), and area under the receiver operating characteristic curve (AUC) of 0.823 (95% CI=0.769-0.887) and 0.803 (95% CI=0.762-0.842) for the training and validation cohorts, respectively. The calibration results indicated favorable agreement between the predicted and actual probability. Furthermore, DCA and clinical impact curve showed the benefit of the nomogram in the clinical decision.
Conclusion: The progression of carotid and coronary plaque was not highly concordant in patients with coronary DES at one-year follow up. The atherosclerotic plaque risk factor (ASPRF) nomogram showed effectively predictive value and clinical utility for the co-progression of carotid and coronary plaque after coronary DES implantation.
Development and utilization of a fluorescence sensor for Zn2+ detection
Authors: Li Xiujuan , Gao Tinghuan , Wang Shoucheng , Zhang Qing , Chen Siyu , Wang Xinchao , Liu Qianqian 
Published: October 2024

Abstract

A fluorescence probe (Probe-Zn) was designed and synthesized for the rapid detection of Zn2+ based on the excited state intramolecular proton transfer (ESIPT) mechanism. The specific recognition ability of Probe-Zn to Zn2+ was tested using UV-VIS and fluorescence emission spectroscopy. The results demonstrated a rapid response within 40s upon addition of an appropriate amount of Zn2+ into the mixed solution of the target probe molecules (DMSO/PBS = 5/5). Under 365 nm ultraviolet light irradiation, the colorless solution changed to yellow-green fluorescence with a 150-folds increase in intensity. Furthermore, the detection limit for specific recognition of Zn2+ by the probe molecule is only 17.3 nmol/L, indicating high sensitivity. The practical application potential of the probe molecules was enhanced by employing on information storage and conducting cell imaging experiments.
Promoter methylation of Retinol Binding Protein 7 (RBP7) predicts worse prognosis in breast cancer patients
Authors: Zhao Xumin , Lu Rong , Chen Pu , Cui Rongrong , Wang Yuanyuan , Hou Peng , Qu Yiping 
Published: October 2024

Abstract

Breast cancer is the most common cancer among women all over the world. Genetic and epigenetic events are accumulated in breast tumorigenesis. Retinoid-binding protein7 (RBP7) is a member of the cellular retinol-binding protein (CRBP) family, which is involved in the pathogenesis of breast cancer. Previous study indicated high expression of RBP7 was significantly related to good relative percent survival in the luminal A subtype. However, promoter methylation of RBP7 and its relationship with clinicopathological features remains unclear. In order to investigate the methylation status of RBP7 in breast cancer patients. We collected and conducted MSP in a cohort of breast cancer patients. Our results showed that high expression of RBP7 correlates with better prognosis in breast cancer from TCGA database. And the MSP experiment in the breast cancer cohort indicated promoter methylation of RBP7 might be one of the reasons causing RBP7 down-regulation in breast cancer patient. Further study indicated that RBP7 methylation was found to be significantly associated with molecular status, LNM and cancer related death. Furthermore, the univariate and multivariable Cox regression analysis showed that RBP7 methylation acted as a predictor of poor survival either in the whole cohort or ER, PR, Her2 negative subtypes. Our study in conclusion indicates that promoter methylation of RBP7 may predict poor prognosis in breast cancer regardless of its molecular status.
Differences in Lower Limb Muscle Activation and Variability Across Walking Speeds by Age and Fall Risk
Authors: Park Yongnam , Bae Youngsook 
Published: October 2024

Abstract

This study aimed to determine the differences in lower limb muscle activation and variability at preferred, slow, and fast walking speeds according to age and fall risk. We divided 301 participants into groups based on age (young older: 70–79 years vs. old older: 80–90 years) and fall risk (fall risk vs. non-fall risk). We measured muscle activation and its coefficients of variation (CV) for the rectus femoris (RF), biceps femoris (BF), tibialis anterior (TA), and medial gastrocnemius muscle (GCM) at speeds 20% slower, 20% faster, and 40% faster than the preferred speed (PS). When compared by age, older adults exhibited greater changes in RF and GCM activities, versus young older adults; however, the CV was not significantly different. Fall risk older adults had significantly lower GCM activity and higher CVs of RF, BF, TA, and GCM in PS. With changes in gait speed, older adults at risk of falling had significantly increased CVs of RF, BF, and GCM. Our findings provide new evidence that variability rather than muscle activity increases with walking speed in older adults at risk of falls, highlighting the importance of decreasing muscle activity variability in preventing fall risk.
The preventive effect of medical biological glue on postoperative bile leakage in laparoscopic common bile duct primary suture
Authors: tian lantian , mi liqiang , li jingxiang , wei wenxin , zhao leitao , yu guangjian , guo yingrong 
Published: October 2024

Abstract

Aim
Laparoscopic common bile duct exploration for stone removal is a relatively comfortable procedure for patients, but the risk of bile leakage after surgery is still a concern. However, the use of medical biological glue in small incisions of the common bile duct may offer a solution. The effectiveness of this method for reducing the occurrence of bile leakage is still uncertain, but it holds great potential for improving patient outcomes. At Juxian People's Hospital, we are committed to exploring all options for enhancing the safety and comfort of our patients.
Objective
To evaluate the value of medical biological glue in preventing biliary fistula in laparoscopic choledochotomy in patients with non-acute suppurative cholangitis.
Methods
Patients undergoing laparoscopic choledochotomy and primary suture were divided into two groups: the control group (group 2 n=24) underwent continuous suture with absorbable sutures, and the experimental group (group 1 n=18) underwent continuous suture with absorbable sutures followed by spraying of medical biological glue. The incidence of biliary fistula, removal time, discharge time, and other indicators were compared between the two groups.
Results
The incidence of biliary fistula, amount of biliary leakage, postoperative hospital stay, and postoperative cost were significantly lower in the biological glue group than in the control group. The use of biological medical glue did not cause any relevant complications, did not affect liver function, did not delay intestinal exhaust time, and the patients felt good overall.
Conclusion
Medical biological glue can significantly reduce the incidence of biliary fistula after laparoscopic choledochotomy and primary suture, without increasing additional complications.
The Effect of Albumin Value, HALP Score and LCR Value on Predicting Survival and Recurrence in Patients with Pancreatic Adenocancer
Authors: GUMUS Tufan , UMMAN Veysel , CETIN Bekir , UGUZ Alper 
Published: October 2024

Abstract

Aim
The aim of this study is to investigate whether the albumin value and the ratios of biochemical markers; hemoglobin, albumin, lymphocyte and platelet (HALP score) and lymphocyte-C-reactive protein ratio (LCR) can predict the survival and recurrence of the disease in patients with pancreatic adenocarcinoma.
Materials and Methods
Patients operated for pancreatic adenocarcinoma (n:87) who did not receive neoadjuvant treatment in our clinic between January 2017 and December 2021 were included in the study. The preoperative albumin levels, HALP scores and LCR values were calculated, and analyzed to determine whether the differences in these ratios could predict pathological findings in the early period.
Results
The average age of 87 patients included in our study was 64.8 ± 9.6 years. 59 (67.8%) of the patients were male and 28 (32.2%) were female. The cut-off value for HALP score was determined as 34.4, the cut-off value for LCR value was 0.61, and the cut-off value for albumin value was 38.55. It was found that the overall survival time of patients with low HALP scores was significantly shorter than patients with high HALP scores (15.8 vs. 19.3 months) (p < 0.01). The overall survival time of patients with low LCR was significantly longer (17.8 vs 18.5 months) than patients with high LCR score (p < 0.01). The overall survival time of patients with high albumin values was found to be significantly longer (14.6 vs 16.3 months) than patients with low albumin values (p < 0.01).
Conclusion
Low HALP levels and low albumin values were confirmed to be significant independent prognostic factors for disease-free survival and overall survival in pancreatic adenocarcinoma patients.
Eye movements follow the dynamic shifts of attention through serial order in verbal working memory
Authors: Schroth Lara Stella Marie , Fias Wim , Sahan Muhammet Ikbal 
Published: October 2024

Abstract

How are arbitrary sequences of verbal information retained and manipulated in working memory? Increasing evidence suggests that serial order in verbal WM is spatially coded and that spatial attention is involved in access and retrieval. Based on the idea that brain areas controlling spatial attention are also involved in oculomotor control, we used eye tracking to reveal how the spatial structure of serial order information is accessed in verbal working memory. In two experiments, participants memorized a sequence of auditory words in the correct order. While their eye movements were being measured, they named the memorized items in a self-determined order in Experiment 1 and in a cued order in Experiment 2. We tested the hypothesis that serial order in verbal working memory interacts with the spatial attention system whereby gaze patterns in visual space closely follow attentional shifts in the internal space of working memory. In both experiments, we found that the gaze shifts in visual space correlated with the spatial shifts of attention along the left-to-right one-dimensional mapping of serial order positions in verbal WM. These findings suggest that spatial attention is employed for dynamically searching through verbal WM and that eye movements reflect the spontaneous association of order and space even in the absence of visuospatial input.
Association of body mass index with clinicopathological features among patients with clear cell renal cell carcinoma treated with surgery: a retrospective study
Authors: Fu Zhibin , Bao Yewei , Dong Kai , Gu Di , Wang Zheng , Ding Jiean , He Ziwei , Gan Xinxin , Wu Zhenjie , Yang Chenghua , Wang Linhui 
Published: October 2024

Abstract

Objective: To investigate the potential association between body mass index (BMI) and the clinicopathological features of patients with clear cell renal cell carcinoma (ccRCC).
Methods: We retrospectively analyzed data from 2451 patients who underwent partial or radical nephrectomy for renal masses between 2013 and 2023 in a single institution. Patients were divided into normal-weight, overweight, and obese groups based on the Chinese BMI classification. Clinicopathological features, including pathologic tumor size, pathologic T (pT) stage, Fuhrman grade or WHO/ISUP grade, renal capsular invasion, perirenal fat or renal sinus fat invasion, and vein cancerous embolus were compared among the groups using Student’s t-test or one-way ANOVA for normally distributed continuous variables, and the chi-square or Fisher’s test for categorical variables.
Results: A total of 2541 ccRCC patients having a median BMI of 24.9 (interquartile range 22.7-27.0) were evaluated. No significant association was found between the pathological tumor diameter and BMI among the normal-weight, overweight, and obese groups (normal-weight vs. overweight, p=0.31; normal-weight vs. obese, p=0.21). There was no statistical difference in pT stage (normal-weight vs. overweight, p=0.28; normal-weight vs. obese, p=0.23). No statistically significant difference was observed in the distribution of Fuhrman/ISUP grade (p=0.12), proportion of patients with renal capsular invasion (p=0.49), perirenal fat or renal sinus fat invasion (p=1.00), and vein cancerous embolus (p=0.64) between the normal-weight and overweight groups. However, patients in the obese group tended to have low Fuhrman or WHO/ISUP grades (p < 0.001), and decreased rates of renal capsular invasion (p < 0.05), perirenal fat or renal sinus fat invasion (p < 0.05), and vein cancerous embolus (p < 0.001).
Conclusions: Obesity was associated with less aggressive pathological features such as low tumor nuclear grade, low rate of renal capsular invasion, perirenal fat or renal sinus fat invasion, and vein cancerous embolus. This finding may provide clinicopathological evidence and explanations for the “obesity paradox” of RCC.