bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1
Authors: Meng Xiangbo , Yang Hui , Chen Feifeng , Li Baohua , Wu Yan , Wang Rong 
Published: October 2024

AbstractBackgroundCerebral ischemic disease is a common cerebrovascular disease, especially ischemic stroke. Exercise has protective functions on brain tissues following cerebral ischemia‐reperfusion injury (CIRI), but its preventive effects and mechanisms in CIRI remain unclear. We aimed to investigate the effects and mechanisms of exercise preconditioning on CIRI.MethodsThe middle cerebral artery occlusion (MCAO) operation was prepared to establish CIRI rats. All rats were randomized into the MCAO, exercise (exercise preconditioning plus MCAO operation), vector (exercise preconditioning, MCAO operation plus intraventricular injection of empty vector), and tissue inhibitor of metalloprotease 1 overexpression (OE‐TIMP1, exercise preconditioning, MCAO operation plus intraventricular injection of OE‐TIMP1) groups.ResultsThe results indicated that exercise preconditioning suppressed approximately 66.67% of neurological deficit scores and 73.79% of TIMP1 mRNA expression in MCAO rats, which were partially offset by OE‐TIMP1. The protective effects of exercise against neuron death status and cerebral infarction size in MCAO rats were reversed by OE‐TIMP1. It also confirmed that exercise weakened apoptosis and oxidative stress damage, with notable increases of B‐cell lymphoma‐2, superoxide dismutase, and glutathione peroxidase production, and evident decreases of BCL2‐associated X, caspase 3, and malondialdehyde in MCAO rats, while these effects were partially reversed by OE‐TIMP1. Additionally, the inhibitory effects of exercise on the protein levels of TIMP1, hypoxia‐inducible factor‐alpha, vascular endothelial growth factor receptor 2, vascular endothelial growth factor, and neurogenic locus notch homolog protein 1 in MCAO rats were partially reversed by OE‐TIMP1.ConclusionAltogether, exercise preconditioning had protective effects on CIRI by restraining TIMP1, which provided new therapeutic strategies for preventing CIRI.
The mediating role of insulin resistance in the association between inflammatory score and MAFLD: NHANES 2017–2018
Authors: Chen Yan , Zhao Xin 
Published: October 2024

AbstractBackgroundThe association between inflammatory score, insulin resistance (IR), and metabolic‐associated fatty liver disease (MAFLD) is inconclusive.ObjectiveThe objective of this study was to examine the relationship between the inflammatory score and MAFLD and investigate the potential mediating effect of IR (evaluated by triglyceride‐glucose index) in this association.MethodsCalculating inflammatory score was performed based on white blood cells and high‐sensitivity C‐reactive protein. The association between the inflammatory score and MAFLD was evaluated based on the weighted multifactor logistic regression model. Restricted cubic splines (RCS) were used to visualize the dose–response relationship between the inflammatory score and MAFLD. We also conducted a mediation analysis to assess the extent to which IR mediates this association.ResultsAmong the 1090 participants, 563 were ultimately diagnosed with MAFLD. Multivariate logistic regression results indicated a close positive association between inflammatory score and MAFLD (odds ratio = 1.235, 95% confidence interval 1.069–1.427, p = .007). The RCS results indicated a linear dose–response relationship between the inflammatory score and the risk of MAFLD after adjusting for potential confounding factors. Furthermore, the mediation analysis results showed that IR partially mediated the association between the inflammatory score and MAFLD (percent mediation = 33%).ConclusionOur research results indicate that the inflammatory score is positively associated with the risk of MAFLD, and IR plays a partial mediating effect in this association.
Higher Temperatures Provide Insights Into the Aerobic Mineralization of Aquatic Macrophyte Leachates
Authors: Freitas Roberta , da Cunha‐Santino Marcela Bianchessi , Bianchini Irineu 
Published: October 2024

ABSTRACTAquatic macrophyte leachate is one of the autochthonous sources of carbon and nitrogen in aquatic systems, and microbial communities easily mineralize these elements. Understanding the effects of climate change on the consumption of dissolved oxygen (DO) due to the mineralization of leachate from aquatic macrophytes is fundamental for accurately establishing the oxygen balance in aquatic systems and forecasting element cycling rates. Bioassays were developed to determine the consumption of DO owing to the mineralization of carbon and nitrogen of the aquatic macrophytes leachates (Myriophyllum aquaticum, Hedychium coronarium, Salvinia auriculata, and Chara sp.) when exposed to a temperature increase of 4°C (from 21°C to 25°C). The results concerning accumulated DO were fitted to a first‐order kinetic model. At 25°C, oxygen consumption due to mineralization increased by 9.6%, whereas the chemical composition of the leachate changed oxygen consumption between 7.2 (21°C) and 9.2% (25°C). The O/C stoichiometry (oxygen consumed by oxidized carbon) indicated the compositions of the leachate, and temperatures determined the pattern of oxygen consumption. Due to the chemical composition of the leachate, the values varied by approximately 17% and were higher at 25°C (about 30%). Regardless, the increase in temperature improved the oxygen consumption of leachate mineralization. After 90 days at 25°C, the highest concentrations of remaining dissolved organic carbon occurred, suggesting the selection of microorganisms and the catabolic routes that favored the production of refractory organic compounds to the detriment of mineralization. The results indicate that knowledge of the effects of climate variations on aquatic systems is crucial for an accurate understanding of the biogeochemical cycles in these environments.
IQL‐OCDA: An intelligent Q‐learning‐based for optimal clustering and data‐aggregation for wireless sensor networks
Authors: Aledaily Arwa N. 
Published: October 2024

AbstractWireless sensor networks (WSNs) can suffer from low battery life due to the energy consumption of the routing protocol. Small sensor nodes are often difficult to recharge after deployment. In a WSN, data aggregation is generally used to reduce or eliminate data redundancy between nodes in order to save energy. In the proposed algorithm, sensor nodes are deployed in appropriate clusters and cluster heads are elected using Q‐learning techniques. Nodes are clustered based on the mean values computed during the clustering phase. Lastly, a performance evaluation and comparison of existing clustering algorithms are performed based on Intelligent Q‐learning. The proposed IQL‐OCDA model reduces end‐to‐end delay by 10.11%, increases throughput by 4.15%, and increases network lifetime by 5.1%.
Approaching ramp lesions from the different world of posterior knee compartment: A review of evidence with a proposal of a new classification and treatment
Authors: Keyhani Sohrab , Mirahmadi Alireza , Maleki Arash , Vosoughi Fardis , Verdonk Rene , LaPrade Robert F. , Landreau Philippe , Movahedinia Mohammad 
Published: October 2024

AbstractRamp lesions (RLs) are peripheral lesions that occur in the posterior part of the medial meniscus or where it attaches to the joint capsule. The classification of the medial meniscus RLs has been the focus of numerous studies and publications. This review provides an overview of RL's current classification and treatment options in anterior cruciate ligament deficient knees. The study also aims to present a more practical classification system for RLs to assist in treatment decision‐making. For the first time, we also presented a new surgical treatment for incomplete inferior and double‐complete RL based on the posterior knee arthroscopy that provides direct access to the posterior meniscal borders, enabling effective treatment and stronger biomechanical repair.Level of EvidenceLevel V.
The Buds of Oscarella lobularis (Porifera, Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology
Authors: Rocher Caroline , Vernale Amélie , Fierro‐Constaín Laura , Séjourné Nina , Chenesseau Sandrine , Marschal Christian , Issartel Julien , Le Goff Emilie , Stroebel David , Jouvion Julie , Dutilleul Morgan , Matthews Cédric , Marschal Florent , Brouilly Nicolas , Massey‐Harroche Dominique , Schenkelaars Quentin , Ereskovsky Alexander , Le Bivic André , Renard Emmanuelle , Borchiellini Carole 
Published: October 2024

ABSTRACTThe comparative study of the four non‐bilaterian phyla (Cnidaria, Placozoa, Ctenophora, and Porifera) provides insights into the origin of bilaterian traits. To complete our knowledge of the cell biology and development of these animals, additional non‐bilaterian models are needed. Given the developmental, histological, ecological, and genomic differences between the four sponge classes (Demospongiae, Calcarea, Homoscleromorpha, and Hexactinellida), we have been developing the Oscarella lobularis (Porifera, class Homoscleromorpha) model over the past 15 years. Here, we report a new step forward by inducing, producing, and maintaining in vitro thousands of clonal buds that now make possible various downstream applications. This study provides a full description of bud morphology, physiology, cells and tissues, from their formation to their development into juveniles, using adapted cell staining protocols. In addition, we show that buds have outstanding capabilities of regeneration after being injured and of re‐epithelization after complete cell dissociation. Altogether, Oscarella buds constitute a relevant all‐in‐one sponge model to access a large set of biological processes, including somatic morphogenesis, epithelial morphogenesis, cell fate, body axes formation, nutrition, contraction, ciliary beating, and respiration.
Association between chronic diseases and severe periodontal disease progression: A retrospective cohort study in a city of Japan
Authors: Nin Sayaka , Sun Yu , Maeno Takami , Nishiura Chihiro , Taira Kento , Fujimoto Kenji , Hamano Jun , Ozone Sachiko , Maeno Tetsuhiro 
Published: October 2024

AbstractBackgroundChronic periodontal disease primarily causes tooth loss and oral frailty and is linked to chronic conditions such as diabetes mellitus. However, its progression and broader studies on chronic diseases have not been well explored. This study aimed to investigate this association using claims data.MethodsThis retrospective cohort study used linked medical, dental, and pharmacy claims data from a local municipality in Japan. The study included participants aged 40–70 years who had received medical care between April 2017 and March 2018. Exposures included age, sex, and common chronic diseases previously reported to be associated with periodontal diseases (21 diseases). We defined the outcome, “progression of severe periodontitis” as the worsening of periodontal disease to a severe stage requiring surgery or tooth extraction, determined by the presence of a periodontal surgery code or a deeper probing pocket depth (≥6 mm) code along with the tooth extraction procedure code. The participants were followed up until March 2022, and multivariate analysis was conducted using Cox proportional hazard models.ResultsAmong 28,846 participants, 1035 (3.6%) progressed to severe periodontal disease. In the multivariate analysis, only diabetes mellitus was significantly associated with severe periodontal disease, with a hazard ratio of 1.26 (95% confidence interval, 1.08–1.53) among all chronic diseases.ConclusionPatients with diabetes mellitus had a high risk of severe periodontal disease progression, suggesting that proactive dental visits should be recommended to prevent severe periodontal disease.
The genomic landscape of transformed splenic diffuse red pulp small B‐cell lymphoma
Authors: Grau Marta , Pol Melina , Montaner Anna , Mozas Pablo , Nadeu Ferran , Márquez‐López Ian , Álamo Jose Ramon , Navarro Alba , Martinez Daniel , Frigola Gerard , Balagué Olga , Lopez‐Guerra Mónica , Colomer Dolors , Ruiz‐Gaspà Silvia , Bashiri Melika , Correa Juan , Giné Eva , López‐Guillermo Armando , Campo Elias , López Cristina , Matutes Estella , Beà Sílvia 
Published: October 2024

AbstractThe genetic landscape underlying the transformation of splenic diffuse red pulp small B‐cell lymphoma (SDRPL) is not well understood. The present study aimed to unravel the genomic alterations involved in the progression and transformation of SDRPL. We performed genetic studies on both SDRPL and subsequent or synchronous diffuse large B cell lymphoma (DLBCL) samples in three SDRPL patients who eventually developed DLBCL. Our findings revealed that SDRPL cases progressing to DLBCL acquired genomic alterations in genes related to the cell cycle (CDKN2A/B, TP53, MYC and CCND3) and B cell development (BCL6).
Susceptibility to lenacapavir, fostemsavir and broadly neutralizing antibodies in French primary HIV‐1 infected patients in 2020–2023
Authors: Chaix Marie‐Laure , Terracol Laura , Nere Marie‐Laure , Stefic Karl , Lascoux‐Combe Caroline , Manda Victoria , Sellier Pierre , Maylin Sarah , Molina Jean‐Michel , Liegeon Geoffroy , Delaugerre Constance , Salmona Maud 
Published: October 2024

AbstractSurveillance studies of Transmitted Drug Resistance (TDR) are crucial in tracking the evolution of HIV epidemiology. Our aim was to investigate TDR to nucleoside reverse transcriptase inhibitors (NRTIs), non‐nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), integrase inhibitors (INIs), as well as to new drugs: lenacapavir, fostemsavir. Predictive sensitivity was evaluated for maraviroc and broadly neutralizing antibodies (bNAbs) (zinlirvimab and teropavimab). Between 2020 and 2023, 85 people with HIV (PWH) were diagnosed with primary HIV‐1 infection (PHI). Pol and env sequences were analyzed and TDR was characterized according to the French ANRS algorithm. The genotypic‐based prediction of bNAbs sensitivity was based on HIV env amino acid signatures I108, I201, F353 for teropavimab and N325, N332, H330 for zinlirvimab. TDR to NRTIs, NNRTIs, PIs and INIs was evidenced in 8.2%, 12.9%, 4.7%, and 5.9% strains, respectively. Ten viruses were CXCR4/dual mix. All viruses were susceptible to lenacapavir (100%) and 52% harbored resistance to fostemsavir. The genotypic profile was associated with a predictive positive value (PPV) > 83% of susceptibility to both teropavimab and zinlirvimab for 23 viruses (31%), while 22 (29%) had a PPV between 62% and 75%, suggesting reduced susceptibility to both bNAbs as soon as primary infection. The surveillance of TDR evidenced at the time of PHI is important with regard to new strategies for HIV patients with virological failure and global implementation of PrEP using NRTI, INI such as recently approved injectable cabotegravir, and future long‐acting drugs such as lenacapavir and bNAbs.
In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold
Authors: Mis Baran , Karaca Kemal , Eltem Rengin 
Published: October 2024

ABSTRACTDuring the cultivation of button mushrooms, the green mold epidemic, which causes a decrease in productivity, is a very important problem. The environmental harm of chemicals used in the control of such epidemics and the demand of consumers for organic products without chemicals have brought environmentally friendly biological control to the fore. Biological control can be achieved by the use of antagonistic microorganisms and their metabolites. In this study, the effectiveness of Bacillus spp. and Pseudomonas spp. for the biological control of the aggressive biotypes of the green mold disease agent Trichoderma aggressivum strains was examined in vitro. For this purpose, the antifungal effects of Bacillus spp. and Pseudomonas spp. against T. aggressivum strains were examined by in vitro dual culture test. Afterward, the antifungal activity of Bacillus spp. metabolites was assessed further using the agar well diffusion method. Then, it was determined whether the bacterial strains showing antifungal activity showed antagonistic activity against A. bisporus. Although none of the Pseudomonas spp. showed antifungal activity against T. aggressivum strains, most of the Bacillus spp. were found to have high activity. It has been concluded that Bacillus sp. Ö‐4‐82 may be potential biological control agent for button mushroom cultivation.
Arctic fishes reveal patterns in radiocarbon age across habitats and with recent climate change
Authors: Stanek Ashley E. , O'Donnell Jonathan A. , Carey Michael P. , Laske Sarah M. , Xu Xiaomei , Dunton Kenneth H. , von Biela Vanessa R. 
Published: October 2024

AbstractClimate change alters the sources and age of carbon in Arctic food webs by fostering the release of older carbon from degrading permafrost. Radiocarbon (14C) traces carbon sources and age, but data before rapid warming are rare and limit assessments over time. We capitalized on 14C data collected ~ 40 years ago that used fish as natural samplers by resampling the same species today. Among resampled fish, those using freshwater food webs had the oldest 14C ages (> 1000 yr BP), while those using marine food webs had the youngest 14C ages (near modern). One migratory species encompassed the entire range of 14C ages because juveniles fed in freshwater streams and adults fed in offshore marine habitats. Over ~ 40 yr, average 14C ages of freshwater and marine feeding fish shifted closer to atmospheric values, suggesting a potential influence from “greening of the Arctic.”
High‐quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures
Authors: Arnold Nathanael D. , Paper Michael , Fuchs Tobias , Ahmad Nadim , Jung Patrick , Lakatos Michael , Rodewald Katia , Rieger Bernhard , Qoura Farah , Kandawa‐Schulz Martha , Mehlmer Norbert , Brück Thomas B. 
Published: October 2024

AbstractThermophilic cyanobacteria thrive in extreme environments, making their thermoresistant enzymes valuable for industrial applications. Common habitats include hot springs, which act as evolutionary accelerators for speciation due to geographical isolation. The family Thermosynechococcaceae comprises thermophilic cyanobacteria known for their ability to thrive in high‐temperature environments. These bacteria are notable for their photosynthetic capabilities, significantly contributing to primary production in extreme habitats. Members of Thermosynechococcaceae exhibit unique adaptations that allow them to perform photosynthesis efficiently at elevated temperatures, making them subjects of interest for studies on microbial ecology, evolution, and potential biotechnological applications. In this study, the genome of a thermophilic cyanobacterium, isolated from a hot spring near Okahandja in Namibia, was sequenced using a PacBio Sequel IIe long‐read platform. Cultivations were performed at elevated temperatures of 40, 50, and 55°C, followed by proteome analyses based on the annotated genome. Phylogenetic investigations, informed by the 16S rRNA gene and aligned nucleotide identity (ANI), suggest that the novel cyanobacterium is a member of the family Thermosynechococcaceae. Furthermore, the new species was assigned to a separate branch, potentially representing a novel genus. Whole‐genome alignments supported this finding, revealing few conserved regions and multiple genetic rearrangement events. Additionally, 129 proteins were identified as differentially expressed in a temperature‐dependent manner. The results of this study broaden our understanding of cyanobacterial adaptation to extreme environments, providing a novel high‐quality genome of Thermosynechococcaceae cyanobacterium sp. Okahandja and several promising candidate proteins for expression and characterization studies.
Application of transcranial Doppler in the diagnosis and management of cervical artery dissection: Insights from a multicenter survey within the STOP‐CAD study
Authors: Guo Xiaofan , Sabayan Behnam , Shifflett Benjamin , Khan Muhib , Antonenko Kateryna , Heldner Mirjam R. , Romoli Michele , Marto João Pedro , Keser Zafer , Rothstein Aaron , Khazaal Ossama , Elnazeir Marwa , Hansra Harjot , Meyer Brett C. , Meyer Dawn M. , Bavarsad Shahripour Reza 
Published: October 2024

AbstractBackgroundCervical artery dissection (CAD) is a common cause of ischemic stroke in young adults. Local blood clot formation and distal emboli are the key mechanisms for the development of stroke in patients with CAD. Microemboli distal to the dissection point can be detected as high‐intensity transient signals (HITS) using transcranial Doppler (TCD). Despite this unique capability, TCD is used inconsistently in the management of CAD. In this international survey, we aim to explore the frequency and potential barriers to TCD use in CAD.MethodThis study was an international electronic survey, based on the Antithrombotic Treatment for Stroke Prevention in Cervical Artery Dissection (STOP‐CAD) study, aiming at evaluating the use of TCD in diagnosing and managing CAD patients. A comprehensive questionnaire was developed to gather insights into the current practices, perceptions, and potential barriers to the effective use of TCD in participating centers. The collected data was then analyzed.ResultsFifty‐eight medical centers with advanced stroke care capabilities from Europe and United States participated in this study. Half (29 out of 58) of the sites routinely perform TCD on CAD patients, particularly in the presence of carotid or vertebral artery stenosis, complete carotid occlusion, and early neurologic deterioration. The primary reason for participating institutions not performing TCD was because its results would not change their practice. The protocol for TCD monitoring and managing HITS‐positive CAD patients varied among centers but transitioning from antiplatelet to anticoagulant was the most common practice in this patient population.ConclusionHalf of the stroke centers in our survey routinely perform TCD monitoring for CAD patients. The detection of HITS in CAD patients is a warning sign for providers, which may prompt a change in secondary stroke prevention management. However, a standard protocol for performing TCD in CAD and treatment recommendations for HITS‐positive CAD are not currently available, which warrants further research.
Weighted multi‐innovation parameter estimation for a time‐varying Volterra–Hammerstein system with colored noise
Authors: Zhao Yanshuai , Ji Yan 
Published: October 2024

AbstractThis article considers the parameter estimation problems of a time‐varying Volterra–Hammerstein (V–H) system with colored noise. We derive a weighted multi‐innovation forgetting factor gradient algorithm by applying the multi‐innovation identification with the weight matrix to adjust the weights of the innovation vector. In order to simplify the identification algorithm and improve the parameter estimation accuracy, the original V–H system with colored noise is separated into two sub‐systems by the hierarchical identification theory. A weighted hierarchical multi‐innovation forgetting factor gradient algorithm is presented for two sub‐systems. Numerical simulation examples test the effectiveness of the proposed algorithms.