bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroeconomic approach to trauma related psychopathology: A version to ambiguous losses in PTSD
Authors: Harpaz-Rotem I. , Jia R. , Ruderman L. , Pietrzak R. , Levy I. 
Published: June 2017

Psychiatric symptoms typically cut across traditional diagnostic categories. In order to devise individually-tailored treatments, there is a need to identify the basic mechanisms that underlie these symptoms. Behavioral and neuro-economics methods provide a framework for studying these potential mechanisms. We utilized this framework to examine aspects of trauma-related symptomatology and its potential link to individual uncertainty attitudes. We distinguish between attitudes towards uncertain outcomes with known (“risk”) and unknown (“ambiguity”) probabilities, and between attitudes towards uncertain gains and uncertain losses. fMRI data were obtained from 57 combat veterans (30 with PTSD, and 27 without PTSD) who made choices involving risky and ambiguous options, which were used to estimate risk and ambiguity attitudes in the gain and loss domains. Veterans with PTSD were more averse to ambiguity, but not risk, compared to veterans without PTSD, when making choices between possible losses, but not gains. The degree of aversion was associated with anxious arousal symptoms, as well as with the degree of combat exposure. A whole brain analysis indicated association between activation in specific brain areas implicated in decision-making and severity of PTSD. Moreover, ambiguity attitudes fully mediated the association between combat exposure and anxious arousal symptoms. These results provide a foundation for the causal association between ambiguity attitudes and trauma-related symptoms, as well as etiology of the neural underpinnings of these behavioral outcomes. Results demonstrate the potential of neuroeconomic and behavioral economic techniques for devising objective and incentive-compatible diagnostic tools, and investigating the etiology of psychiatric disorders.Disclosure of interestThe authors have not supplied their declaration of competing interest.
Impact of emotional and cognitive saliency on visual search in post-traumatic stress disorder
Authors: Kiesmann E. , Grandgenèvre P. , Mallet J. , Dubertret C. , Vaiva G. 
Published: June 2017

IntroductionPatients with post-traumatic stress disorder (PTSD) have shown disturbances in visual information treatment. However, most of studies demonstrated attentional bias towards emotional stimuli by using non-ecological paradigm. The paradigm of change blindness offers the possibility of studying sensitivity to the sudden irruption of visual information with ecological stimuli.Objectives/AimsTo compare the explicit detection with the implicit detection by using respectively motor response and eye tracking in patients with PSTD and controls.MethodsFifteen patients with PTSD and fifteen healthy controls had to detect changes in 96 scenes with (1) no change, (2) one neutral change or (3) one emotional pleasant or unpleasant change. We measured the participant's speed and accuracy in explicitly reporting the changes via motor responses, and their capacity to implicitly detect changes via eye movements.ResultsThe patients showed a trend towards slower explicit detection for the emotional change (P = 0.06) and more specifically for unpleasant change (P = 0.054). The two groups did not differ for implicit detection.ConclusionPatients tend to explicitly detect more slowly emotional change (but not neutral), especially for unpleasant change. This could be the result of a lack of access to consciousness of the emotional information. The emotional visual information treatment in PSTD could require more attentional processes than the non-emotional visual information and then lead to a decrease of the available attentional resources for the explicit task.Disclosure of interestThe authors have not supplied their declaration of competing interest.
Anti-inflammatory properties of brilliant blue G on chronic unpredictable mild stress-induced changes in rat hippocampus
Authors: Aricioglu F. , Bastaskin T. , Kandemir C. , Sirvanci S. , Ozkartal C. , Utkan T. 
Published: June 2017

ObjectivePurinergic 2X7 receptor (P2X7R) activation has recently been considered to be involved in depression at least partially by triggering microglial activation. The aim of the present study was to examine whether the chronic administration of brilliant blue G (BBG), a highly selective P2X7R antagonist, has antidepressant-like effects and microglial (Iba-1) immunoreactivity in chronic unpredictable mild stress (CUMS) model in rats.MethodsMale Wistar Albino rats (290–360 g) were divided into groups such as control (saline), CUMS, CUMS + Imipramine (20 mg/kg; i.p.), CUMS + BBG25 (25 mg/kg; i.p.), CUMS + BBG50 (50 mg/kg; i.p.) groups (n = 10–12 in each). In CUMS model, various stressors were applied for 40 days. On day 20, the treatment of BBG was started for 20 days. At the end, sucrose preference and forced swimming tests were performed. Then brains were removed with paraformaldehyde perfusion for Iba-1 immunohistochemical analysis in hippocampus. One-way analysis of variance and Tukey's test were used for statistical analysis.ResultsThe time of immobility in forced swim test was significantly reduced while sucrose preference was increased in Imipramine and CUMS + BBG50 groups compared to control and CUMS groups, respectively. In immunohistochemical experiments, Iba-1 was overexpressed in CUMS group and BBG significantly reduced the overexpression of Iba-1.ConclusionOur results suggest that chronic administration of BBG has an antidepressant-like activity supporting the notion of P2X7 receptors involvement in depression by modulating microglial activation.This research was supported by grant from Marmara University, Scientific Research Projects – SAG-C-YLP-110915-0416 and SAG-E-120613-0233.Disclosure of interestThe authors have not supplied their declaration of competing interest.
Zero suicide southwest UK initiative – Steps to mitigate suicide risk in local populations using quality improvement methodology and a whole life approach
Authors: Shankar R. , Wilkinson E. , Roberts S. , Rebecca O. 
Published: June 2017

BackgroundOnly 25% of people who die by suicide see mental health services. Suicide is not just a health issue. Its causation and consequences lie within all of society. Many erroneously believe that suicide is inevitable and not preventable, because its causation is too complex. Underlying associations with suicide are largely social. There are programmes in the USA, which have combined interventions to reduce suicides. The 2014 UK suicide rate per 100,000 was 10.8 but 11.1 in South West (SW) England (pop: 5 million). A whole system approach is necessary. Zero Suicides SW is a project to address this.Aim(1) To develop a regional strategy to reduce and prevent suicide. (2) To make whole populations suicide risk aware. (3) Reduce regional suicide rates.MethodA collaborative involving national and local 60 organisations including charities and voluntary sector was formed. Five collaborative meetings used narratives of suicide survivors, national experts led themed workshops, etc. to come up with a regional strategy. Quality Improvement (QI) Methodology was used to develop and examine the success of all projects.OutputsInitiatives such as using local radio stations for mental health promotion, collaboration via a poster campaign with local breweries and pubs to make men more self-aware of risk, suicide risk counselling for relatives/carers of patients admitted to psychiatric care, improving scrutiny to access to medication for recently discharged psychiatric patients have developed from the project. The QI model demonstrated how localised changes at person and organisation level could combine and have a powerful role in suicide prevention.Disclosure of interestThe authors have not supplied their declaration of competing interest.
Diurnal variation of intraoral pH and temperature
Authors: Choi Jung Eun , Lyons Karl M , Kieser Jules A , Waddell Neil J 
Published: June 2017

Abstract
Objective/Aims:
The aim of this study was to measure continuously the intraoral pH and temperature of healthy individuals to investigate their diurnal variations.

Materials and Methods:
Seventeen participants (mean age, 31±9 years) wore a custom-made intraoral appliance fitted with a pH probe and thermocouple for two sets of 24 h, while carrying out normal daily activities including sleep. The continuous changes in intraoral pH and temperature were captured using a sensor placed on the palatal aspect of the upper central incisors. The collected data were categorised into different status (awake and sleep) and periods (morning, afternoon, evening and night). Both quantitative and qualitative analyses were conducted.

Results:
The intraoral pH change was found to show a distinctive daily rhythm, showing a 12-h interval between maximum (7.73) and minimum (6.6) pH values. The maximum and minimum values were found to repeat after 24 h. The mean pH over 48 h (two sets of 24 h) was found to be 7.27 (±0.74). There was significant difference found in pH when subjects were awake and asleep and different periods during the day (P<0.001). The mean intraoral temperature was 33.99 °C (±4.9), with less distinctive daily rhythm compared with pH. There was a significant difference found in temperature depending on the time of the day, except between morning and afternoon (P=0.78).

Conclusion:
Our results showed that there is a distinctive daily, circadian-like pattern in intraoral pH variation over a 24-h period, which has been considered as one of the risk factors in sleep-related dental diseases.
Hot electrons in water: injection and ponderomotive acceleration by means of plasmonic nanoelectrodes
Authors: Zilio Pierfrancesco , Dipalo Michele , Tantussi Francesco , Messina Gabriele C , de Angelis Francesco 
Published: June 2017

AbstractWe present a theoretical and experimental study of a plasmonic nanoelectrode architecture that is able to inject bunches of hot electrons into an aqueous environment. In this approach, electrons are accelerated in water by ponderomotive forces up to energies capable of exciting or ionizing water molecules. This ability is enabled by the nanoelectrode structure (extruding out of a metal baseplate), which allows for the production of an intense plasmonic hot spot at the apex of the structure while maintaining the electrical connection to a virtually unlimited charge reservoir. The electron injection is experimentally monitored by recording the current transmitted through the water medium, whereas the electron acceleration is confirmed by observation of the bubble generation for a laser power exceeding a proper threshold. An understanding of the complex physics involved is obtained via a numerical approach that explicitly models the electromagnetic hot spot generation, electron-by-electron injection via multiphoton absorption, acceleration by ponderomotive forces and electron-water interaction through random elastic and inelastic scattering. The model predicts a critical electron density for bubble nucleation that nicely matches the experimental findings and reveals that the efficiency of energy transfer from the plasmonic hot spot to the free electron cloud is much more efficient (17 times higher) in water than in a vacuum. Because of their high kinetic energy and large reduction potential, these proposed wet hot electrons may provide new opportunities in photocatalysis, electrochemical processes and hot-electron driven chemistry.
The role of city size and urban form in the surface urban heat island
Authors: Zhou Bin , Rybski Diego , Kropp Jürgen P. 
Published: June 2017

AbstractUrban climate is determined by a variety of factors, whose knowledge can help to attenuate heat stress in the context of ongoing urbanization and climate change. We study the influence of city size and urban form on the Urban Heat Island (UHI) phenomenon in Europe and find a complex interplay between UHI intensity and city size, fractality, and anisometry. Due to correlations among these urban factors, interactions in the multi-linear regression need to be taken into account. We find that among the largest 5,000 cities, the UHI intensity increases with the logarithm of the city size and with the fractal dimension, but decreases with the logarithm of the anisometry. Typically, the size has the strongest influence, followed by the compactness, and the smallest is the influence of the degree to which the cities stretch. Accordingly, from the point of view of UHI alleviation, small, disperse, and stretched cities are preferable. However, such recommendations need to be balanced against e.g. positive agglomeration effects of large cities. Therefore, trade-offs must be made regarding local and global aims.
Development of squaraine based G-quadruplex ligands using click chemistry
Authors: Zhang Xin , Wei Yongbiao , Bing Tao , Liu Xiangjun , Zhang Nan , Wang Junyan , He Junqing , Jin Bing , Shangguan Dihua 
Published: June 2017

AbstractThe G-quadruplex (G4) structures of nucleic acids are considered to play an intrinsic role in gene expression. To this end, the development of new G4 ligands has attracted extensive research interests towards potential applications as G4-targeted drugs and molecular probes. To date, the majority of G4 ligands have been composed of an extended planar aromatic scaffold that interacts with the terminal G-tetrad plane via π-π interactions, and various side chains that interact with the sugar-phosphate backbone, loops or grooves of the G4 structures. The side chains act to modulate the affinity and selectivity of the G4 ligands, alongside influencing their biodistribution. Here, we present a click chemistry methodology to generate a series of squaraine-based G4 ligand derivatives based on our previously reported G4 probe (named CSTS) but with varing side chains. We find that importantly these new G4 ligand derivatives retain the G4 selectivity, optical properties and low cytotoxicity of CSTS, but exhibit different binding behaviors to G4 structures, and distinct cellular uptake efficiencies. Indeed, of these new complexes, several exhibit much higher affinity and cellular uptake than CSTS. Overall, this novel, facile and highly effective strategy has significant future potential for the high-throughput screening of G4 ligands or probes targeted towards in vivo applications.
Ultra-broadband, wide angle absorber utilizing metal insulator multilayers stack with a multi-thickness metal surface texture
Authors: Ghobadi Amir , Dereshgi Sina Abedini , Hajian Hodjat , Bozok Berkay , Butun Bayram , Ozbay Ekmel 
Published: June 2017

AbstractIn this paper, we propose a facile route to fabricate a metal insulator multilayer stack to obtain ultra-broadband, wide angle behavior from the structure. The absorber, which covers near infrared (NIR) and visible (Vis) ranges, consists of a metal-insulator-metal-insulator (MIMI) multilayer where the middle metal layer has a variant thickness. It is found that this non-uniform thickness of the metal provides us with an absorption that is much broader compared to planar architecture. In the non-uniform case, each thickness is responsible for a specific wavelength range where the overall absorption is the superposition of these resonant responses and consequently a broad, perfect light absorption is attained. We first numerically examine the impact of different geometries on the overall light absorption property of the multilayer design. Afterward, we fabricate the designs and characterize them to experimentally verify our numerical findings. Characterizations show a good agreement with numerical results where the optimum absorption bandwidth for planar design is found to be 620 nm (380 nm–1000 nm) and it is significantly boosted to an amount of 1060 nm (350 nm–1410 nm) for multi-thickness case.
Interspecific comparison of allometry between body weight and chest girth in domestic bovids
Authors: Anzai Hiroki , Oishi Kazato , Kumagai Hajime , Hosoi Eiji , Nakanishi Yoshitaka , Hirooka Hiroyuki 
Published: June 2017

AbstractThe sizes of body parts often co-vary through exponential scaling, known as allometry. The evolution of allometry is central to the generation of morphological diversity. To make inferences regarding the evolved responses in allometry to natural and artificial selection, we compared allometric parameters (slope and intercept) among seven species and breeds of domestic bovids using cross-sectional ontogenetic data and attempted to interpret the differences in these parameters. The allometric slopes were not different among some species, whereas those between breeds within species were, indicating that the slopes were typically invariant but could be changed under strong, specific selection. With the exception of yak, the differences in the intercept independent of the slopes (the alternative intercept) among species might better correspond to their divergence times than the differences in allometric slope, and the remarkably higher alternative intercept found in yaks can be explained by their unique morphological evolution. These findings provide evidence that differences in the alternative intercept can retain traces of the phylogenetic changes derived from differentiation and evolution.
Probing the missing mature β-cell proteomic landscape in differentiating patient iPSC-derived cells
Authors: Vethe Heidrun , Bjørlykke Yngvild , Ghila Luiza M. , Paulo Joao A. , Scholz Hanne , Gygi Steven P. , Chera Simona , Ræder Helge 
Published: June 2017

AbstractMODY1 is a maturity-onset monogenic diabetes, caused by heterozygous mutations of the HNF4A gene. To date the cellular and molecular mechanisms leading to disease onset remain largely unknown. In this study, we demonstrate that insulin-positive cells can be generated in vitro from human induced pluripotent stem cells (hiPSCs) derived from patients carrying a non-sense HNF4A mutation, proving for the first time, that a human HNF4A mutation is neither blocking the expression of the insulin genes nor the development of insulin-producing cells in vitro. However, regardless of the mutation or diabetes status, these insulin-producing cells are immature, a common downfall off most current β-cell differentiation protocols. To further address the immature state of the cells, in vitro differentiated cells and adult human islets were compared by global proteomic analysis. We report the predicted upstream regulators and signalling pathways characterizing the proteome landscape of each entity. Subsequently, we focused on the molecular components absent or misregulated in the in vitro differentiated cells, to probe the components involved in the deficient in vitro maturation towards fully functional β-cells. This analysis identified the modulation of key developmental signalling pathways representing potential targets for improving the efficiency of the current differentiation protocols.
Downregulation of ATG5-dependent macroautophagy by chaperone-mediated autophagy promotes breast cancer cell metastasis
Authors: Han Qi , Deng Youcai , Chen Sha , Chen Rui , Yang Mingzhen , Zhang Zhujun , Sun Xiongshan , Wang Wei , He Ying , Wang Fangjie , Pan Xiaodong , Li Peng , Lai Wenjing , Luo Hongqin , Huang Pei , Guan Xiao , Deng Yafei , Yan Jun , Xu Xianjie , Wen Yan , Chen An , Hu Chuanmin , Li Xiaohui , Li Shuhui 
Published: June 2017

AbstractRecent data have shown that the expression of lysosome-associated membrane protein type 2 A (LAMP2A), the key protein in the chaperone-mediated autophagy (CMA) pathway, is elevated in breast tumor tissues. However, the exact effects and mechanisms of CMA during breast cancer metastasis remain largely unknown. In this study, we found that the LAMP2A protein level was significantly elevated in human breast cancer tissues, particularly in metastatic carcinoma. The increased LAMP2A level was also positively correlated with the histologic grade of ductal breast cancer. High LAMP2A levels also predicted shorter overall survival of breast cancer patients. Downregulation of CMA activity by LAMP2A knockdown significantly inhibited the growth and metastasis of both MDA-MB-231 and MDA-MB-468 breast cancer cells in vivo and in vitro, while upregulation of CMA activity by LAMP2A overexpression had the opposite effect. Mechanistically, we found that elevated CMA activity mediated increased growth and metastasis of human breast cancer cells by downregulating the activity of autophagy-related gene 5 (ATG5)-dependent macroautophagy. Collectively, these results indicate that the anti-macroautophagic property is a key feature of CMA-mediated tumorigenesis and metastasis and may, in some contexts, serve as an attractive target for breast cancer therapies.
Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
Authors: Seifert Jan , Rheinlaender Johannes , Lang Florian , Gawaz Meinrad , Schäffer Tilman E. 
Published: June 2017

AbstractPlatelets are small anucleate blood cells involved in haemostasis. Platelet activation, caused by agonists such as thrombin or by contact with the extracellular matrix, leads to platelet adhesion, aggregation, and coagulation. Activated platelets undergo shape changes, adhere, and spread at the site of injury to form a blood clot. We investigated the morphology and morphological dynamics of human platelets after complete spreading using fast scanning ion conductance microscopy (SICM). In contrast to unstimulated platelets, thrombin-stimulated platelets showed increased morphological activity after spreading and exhibited dynamic morphological changes in the form of wave-like movements of the lamellipodium and dynamic protrusions on the platelet body. The increase in morphological activity was dependent on thrombin concentration. No increase in activity was observed following exposure to other activation agonists or during contact-induced activation. Inhibition of actin polymerization and inhibition of dynein significantly decreased the activity of thrombin-stimulated platelets. Our data suggest that these morphological dynamics after spreading are thrombin-specific and might play a role in coagulation and blood clot formation.
Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition
Authors: Zito Giovanni , Naselli Flores , Saieva Laura , Raimondo Stefania , Calabrese Giovanna , Guzzardo Claudio , Forte Stefano , Rolfo Christian , Parenti Rosalba , Alessandro Riccardo 
Published: June 2017

AbstractA fundamental task in cancer research aims at the identification of new pharmacological therapies that can affect tumor growth. Differentiation therapy might exploit this function not only for hematological diseases, such as acute promyelocytic leukemia (APML) but also for epithelial tumors, including lung cancer. Here we show that Retinoic Acid (RA) arrests in vitro and in vivo the growth of Tyrosine Kinase Inhibitors (TKI) resistant Non Small Cell Lung Cancer (NSCLC). In particular, we found that RA induces G0/G1 cell cycle arrest in TKI resistant NSCLC cells and activates terminal differentiation programs by modulating the expression of GATA6, a key transcription factor involved in the physiological differentiation of the distal lung. In addition, our results demonstrate that RA inhibits EGFR and Wnt signaling activation, two pathways involved in NSCLC progression. Furthermore, we uncovered a novel mechanism in NSCLC that shows how RA exerts its function; we found that RA-mediated GATA6 activation is necessary for EGFR and Wnt inhibition, thus leading to 1) increased differentiation and 2) loss of proliferation. All together, these findings prove that differentiation therapy might be feasible in TKI resistant NSCLCs, and shed light on new targets to define new pharmacological therapies.