bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Causal Role of Uterine Fibroid in Keloid and Hypertrophic Scar: A Bidirectional Mendelian Randomization Study on European Populations
Authors: Zhou Xiaobo , Lin Jui-Ming , Wang Hui , Gong Yiyi , Lin Jinran , Wu Wenyu , Huang Jia 
Published: September 2024

Background:
The relationship between uterine fibroids and keloid/hypertrophic scars
has been contradictory. Our research employs a bidirectional Mendelian Randomization (MR)
approach to establish a clearer understanding of this potential causal link.

Objective:
This study aimed to determine the effect of uterine fibroids on keloid/hypertrophic
scars and the effect of keloid/hypertrophic scars on uterine fibroids.

Purpose:
We aimed to demonstrate the relationship between uterine fibroids and keloid/
hypertrophic scars.

Method:
Our bidirectional MR study utilized summarized data from genome-wide association
studies (GWAS) focused on European populations. Our primary tool for establishing causality
was the Inverse-Variance Weighted (IVW) method. To reinforce the IVW findings, we also applied
four alternative MR methods: MR-Egger, Maximum Likelihood, Weighted Mode, and
Weighted Median.

Result:
The IVW method indicated a significant causal link, with uterine fibroids greatly raising
the likelihood of developing keloids (Odds Ratio [OR] = 1.202, 95% Confidence Interval [CI]:
1.045-1.381; P=0.010) and hypertrophic scars (OR = 1.256, 95% CI: 1.039-1.519; P=0.018).
Parallel results were observed with the MR-Egger, Maximum Likelihood, Weighted Mode, and
Weighted Median methods. Sensitivity analyses indicated robustness in these findings, with no
evidence of heterogeneity or horizontal pleiotropy. Conversely, the reverse MR analysis did not
demonstrate an increased risk of uterine fibroids due to keloids or hypertrophic scars.

Conclusion:
This study elucidates a significant causal effect of uterine fibroids on the development
of keloid and hypertrophic scars, offering valuable insights into their pathogenesis and
potential therapeutic targets.
Deoxypodophyllotoxin Mediates Autophagy Death through Inhibition of
GRP78 in Human Osteosarcoma
Authors: Tang Xiao-jun , Luo Ling-li , Zhou Wen-chao , Shi Gang-qing , Wang Xiao-xu , Zeng Tian , Tan Xiong-jin , Guo Wei-ming , Gao An-bo , Li Yukun , Zou Juan 
Published: September 2024

Background:
Glucose-regulated protein 78 (GRP78), as a chaperone protein, can
protect the endoplasmic reticulum of cells and is expressed to influence chemoresistance and
prognosis in cancer. Deoxypodophyllotoxin (DPT) is a compound with antitumor effects on
cancers. DPT inhibits the proliferation of osteosarcoma by inducing apoptosis, necrosis, or
cell cycle arrest.

Object:
This study was performed to demonstrate the molecular mechanism by which DPT
attenuates osteosarcoma progression through GRP78.

Methods:
Natural compound libraries and western blot (WB) were used to screen the inhibitors of osteosarcoma GRP78. The expression of mitochondria-related genes in cancer cells of
the treatment group was detected by quantitative real-time PCR (qPCR) and WB. 3-(4,5)-
Dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) and 5-ethynyl-2'-
deoxyuridine (EDU) were used to discover the activity and proliferation of osteosarcoma
cells treated with DPT. We constructed an in vivo mouse model of DPT drug therapy and carried out immunohistochemical detection of xenografts. The treated osteosarcoma cells were
analyzed using bioinformatics and electron microscopy. The data were analyzed finally

Results:
DPT inhibited osteosarcoma cell survival and the growth of tumor xenografts. It
promoted up-regulation of BCL2-associated X protein (Bax) and B-cell CLL/lymphoma 2
(Bcl-2), which serves to mediate and attenuate, respectively, the killing activities of DPT
through mitochondria dysfunction. The effect of DPT against cancer cells could be attenuated
by the overexpression of GRP78, characterized by the inactivation of the caspase cascade.
The loss of GRP78 in osteosarcoma cells negatively mediated the basal level of autophagyassociated genes. DPT stimulated autophagy via the phosphoinositide 3-kinase (PI3K)-v-akt
murine thymoma viral oncogene homolog (AKT), a mechanistic target of rapamycin (mTOR)
axis. The autophagy caused by DPT played an active role in the osteosarcoma of humans and
blocked the apoptotic cascade.

Conclusion:
Combination treatment with the GRP78 inhibitor DPT and pharmacological autophagy inhibitors will be a meaningful method of obviating osteosarcoma cells.
In Silico Analysis and Molecular Docking of Human Antimicrobial Peptides for Targeting Monkeypox Virus: Potential Therapeutic Implications of Histatin 5 Peptide
Authors: Radhakumar Deeksha Sarojini Devi , Thiyagarajan Sundar , Rajaram Kaushik , Parsanathan Rajesh 
Published: September 2024

Background:
Monkeypox, a viral zoonotic disease akin to smallpox, has posed significant
public health challenges, particularly in Africa. Recent outbreaks, including those in India,
underscore the global threat it poses.

Objective:
In this study, we explore a novel approach to combat monkeypox virus (MPXV) infection
by targeting its surface proteins, crucial for viral entry and fusion.

Methods:
Employing advanced computational techniques, we predict and refine the 3D structures
of MPXV surface proteins and human antimicrobial peptides (hAMPs), specifically Histatin 1, 3,
and their cleaved product, Histatin 5 (HIS 5). Further, molecular docking was carried out for
MPXV surface proteins with hAMP HIS using HDOCK and Cluspro 2.0. Protein-peptide interactions
were analyzed using PdbSum. Finally, the physicochemical properties of HIS peptides were
determined using CamSol.

Results:
Our findings suggest HIS 5 as a potential therapeutic peptide against MPXV, warranting
further investigation through in vitro and in vivo studies.

result:
Compared to HIS 1 and 3, HIS 5 has higher solubility, an estimated half-life, cell permeability, stability, and a good docking score. Hence, based on the physicochemical properties of HIS 5, it may be used as a novel potential therapeutic peptide against MPXV.

Conclusion:
This study sheds light on the efficacy of the HIS family in targeting MPXV and advocates
for continued exploration of HIS 5's antiviral effects.
Synthesis of Palladium Nanomaterials Using Plant Leaf Extract and their Enhanced Photocatalytic Activities against Organic Dyes
Authors: Kaur Gurcharn , Aulakh Jatinder Singh 
Published: September 2024

Aims and Objectives:
This study aimed to carry out the green synthesis of palladium nanoparticles
(Pd NPs) using the aqueous leaves extract of Eclipta alba. The appearance of a characteristic
surface plasmon resonance peak at 410 nm confirmed the synthesis of Pd NPs.

Method:
The average particle sizes of 13 nm were observed by using the leaf concentrations of 10
mL with a certain amount of PdCl2 (0.001 M) at 25 ̊ C. The Pd NPs, as synthesized under the optimized
conditions (10 mL extract + 60 ̊ C + 0.001 M PdCl2), were spherical in shape, small in size,
and uniformly distributed, as depicted by HR-TEM images.

Results:
FTIR, XRD, DLS, and zeta potential further confirmed the formation of Pd NPs. The Pd
NPs synthesized at optimized conditions exhibited strong catalytic activity in dye Eosin yellow, Rose
Bengal, Tartrazine, and Ponceau S degradation by discoloration of dyes in 3 hrs. The removal of all
dyes by the Pd NPs was optimized by varying specific operating parameters, such as initial dye concentration,
solution pH, irradiation time, and temperature.

Conclusion:
This high activity of Pd NPs may be due to their small size, high dispersion, and surface-
capping phytochemicals.

result:
Bio-fabrication of metallic Pd NPs using different plant extracts compared to other reducing agents is more scalable, rapid, and cost-effective for generating bulk nanoparticles. The phytochemical principles, such as phytoconstituents, reduce the PdCl2 to Pd NPs and are implicated in the capping and stability of the nanoparticles. Hence, the leaves of Eclipta alba have different polyols and are well known. Apart from this, hydroxyl groups in phenolic compounds may participate in the bio-reduction of PdCl2. Such compounds can chelate metal ions to form their quinones form, which is an intermediate palladium complex. Then, stabilization of the intermediate form and reduction of Pd2+ to Pd0 occurred due to free electrons or nascent hydrogen produced during the bio-reduction reaction. Finally, Pd-NPs were prepared due to the collision of neighboring Pd0 atoms. These significant conjugations exist in the keto-enol system within the quinone form of the compound.
The Acute Kidney Injury Induced by Cisplatin in Mice is Exacerbated by the Conditioned Medium Derived from the Cultivation of Mesenchymal
Stem Cells under Hypoxic Conditions
Authors: Temnov Andrey A. , Sklifas Alla N. , Sharapov Mars G. , Fadeev Roman S. , Klychnikova Elena V. , Dolbnya Andrey D. , Pekov Stanislav I. , Popov Igor A. 
Published: September 2024

Background:
Acute Kidney Injury (AKI) is a severe complication of cisplatin-based
chemotherapy. Thus, searching for novel therapeutic approaches to reduce system toxicity is vital
for improving patient outcomes. The use of stem cells or the paracrine factors released by these
cells during cultivation is currently being explored as a potential method for AKI prevention during
chemotherapy. However, the conditions of stem cell cultivation considerably affect the composition
of paracrine factors released by cells.

Objective:
In this study, we aimed to investigate the impact of paracrine factors derived from mesenchymal
stem cells cultured under hypoxic conditions on the progression of AKI induced by cisplatin.

Methods:
AKI was induced in mice by intraperitoneal administration of cisplatin with the simultaneous
injection of fractions of conditioned medium obtained from the cultivation of mesenchymal
stem cells under hypoxic conditions. The survival rate of animals was assessed alongside qRTPCR
implementation to assess gene expression of cytokines.

Results:
The total fraction of conditioned medium and >30 kDa fraction had no impact on cisplatin
nephrotoxicity in mice. However, either subcutaneous or intraperitoneal administration of
<30 kDa fraction of conditioned medium exacerbated animal mortality and led to severe damage
to renal tissues. The effect was in a good correlation with KIM-1 and CDKN1A gene expression.

Conclusion:
The conditioned medium obtained during mesenchymal stem cells under hypoxic
conditions has been found to markedly amplify the toxicity of cisplatin, which should be considered
in stem cell therapy of AKI patients.
Evaluating the Reliability of Machine Learning Predictors in m6A-SNP Association Analysis: A Comparative Study Using m6A-QTL Data
Authors: Mao Zhongzheng , Wei Zhen 
Published: September 2024

Introduction:
N6-Methyladenosine (m6A) plays a crucial role in determining the fate of
RNA after transcription. Understanding the downstream functions of individual m6A sites is of critical
interest in epitranscriptomics. In published studies, two main approaches have been used to decipher
the specific impact of m6A sites on gene expression and disease/traits: the m6A quantitative
trait loci (m6A-QTL) and in-silico mutation prediction by Machine Learning (ML) models. However,
earlier works still lack independent validation for the performance of ML-based methods.

Methods:
In this study, we use m6A-QTL as ground truth to evaluate the outcomes of in-silico mutation
models. We benchmark both the newly trained machine learning models using genomic or
sequence features and the existing model inference results published in in-silico mutationdependent
databases against m6A-QTL.

Results:
We found that the consistency between in-silico mutation and m6A-QTL is weak, regardless
of the ML algorithms and predictive features used. This trend was also consistent across multiple
published databases based on in-silico mutation, including RMDisease2, m6AVar, and RMVar.

Conclusion:
These results highlight the importance of critical empirical evaluations for ML models
in future SNP-m6A association studies and suggest the need for more high-quality m6A-QTL experiments
to guide model development.
Mechanisms of Cinnamomi Cortex against Diabetes Mellitus Explored by Network Pharmacology combined with Molecular Docking and Experimental Validation
Authors: Yu Jianqin , Song Zijun , Wang Lusheng , Yang Hongyu , Fan Hui 
Published: September 2024

Objective:
Cinnamomi cortex (CC), a traditional Chinese herbal medicine, exhibits
antidiabetic properties, yet the underlying mechanisms are not fully understood. Our study
combined network pharmacology, molecular docking, and experimental validation to elucidate
the antidiabetic mechanisms of CC.

Methods:
Active components of CC and their potential antidiabetic targets were identified
through TCMSP, DisGeNET, and GeneCards. The PPI networks were constructed with
STRING and analyzed with Cytoscape, while GO and KEGG analyses utilized the DAVID database.
Molecular docking with core targets was performed using Autodock Vina. The efficacy
of CC in diabetes mellitus was evaluated through H&E staining, qPCR, and Western blot in the
T2DM mouse.

Results:
Eleven active components and sixty-six potential antidiabetic targets of CC were identified.
The enrichment analysis revealed 288 GO terms and 37 pathways. The molecular docking
showed high affinity for PPAR-γ and IL-6 receptors. In vivo studies further confirmed CC's
ability to modulate PPAR-γ and IL-6, contributing to its antidiabetic effects.

Conclusion:
CC manages diabetes by regulating the PPAR-γ pathway and suppressing associated
inflammation, providing a multi-pathway therapeutic approach.
Likelihood of Laryngeal Involvement in Autoimmune Thyroid Disorder Patients: A Meta-Analysis
Authors: Yue Chuan , Gu Li , Guo Wei 
Published: September 2024

Background:
The involvement of the larynx in many situations can have a substantial
impact on a person's voice, breathing, and general health. Individuals with autoimmune thyroid
disorders can experience a variety of conditions affecting the larynx. Autoimmune thyroid
disorders, such as Hashimoto's thyroiditis [HT] and Graves' disease [GD], are prevalent conditions
affecting the thyroid gland. Beyond their established impact on thyroid function, these disorders
have been associated with laryngeal involvement. The current study aims to explore the
likelihood of laryngeal involvement in patients with Autoimmune Thyroid Disorders [AITD].

Method:
This study involved a retrospective analysis of medical records from patients diagnosed
with autoimmune thyroid disorders. Inclusion criteria were a confirmed diagnosis through
laboratory investigations and clinical assessment. Patients with pre-existing laryngeal pathologies
or other conditions affecting the larynx were not considered. We collected data from 4 research
articles and 3 clinical trials from Embase, PubMed, and NCBI-Trials portals, focusing on
reported laryngeal symptoms. The severity of laryngeal involvement was assessed and categorized
based on its extent and impact on vocal function.

Results:
Preliminary analysis of the collected data indicated a significant proportion of patients
with autoimmune thyroid disorders reporting laryngeal symptoms. Among these patients, various
manifestations of laryngeal involvement were observed, including vocal changes, hoarseness,
and throat discomfort.

Conclusion:
The findings show that laryngeal symptoms may be an underappreciated feature of
these diseases, potentially impacting vocal function and quality of life in affected people. Further
research is also needed for more precise projections in this direction.
LINCRNA01094 Promotes Renal Interstitial Fibrosis via the Mir-513b-5p/MELK/Smad3 Axis
Authors: Zhang Xingguang , Jia Binghan , Zhang Yanqi , Zhang Sen 
Published: September 2024

Background:
Chronic Kidney Disease (CKD) is a common chronic disease that is a
threat to human health. Accumulating evidence showed that long noncoding RNAs (lncRNAs)
are associated with various diseases and can function as competing endogenous RNAs (ceRNAs).
However, the roles and functions of the lncRNA‒miRNA-mRNA network in CKD are
still unclear.

Methods:
In this study, we performed differential expression analysis of lncRNAs, miRNAs,
and mRNAs in CKD using the datasets GSE66494 and GSE80247 from the Gene Expression
Omnibus. A total of 33 lncRNAs, 20 miRNAs, and 240 mRNAs were differentially expressed
between CKD patients and healthy controls. Two ceRNA interaction modules composed of 11
hub nodes, namely, 2 lncRNAs (LINC01086, LINC01094), 2 miRNAs (hsa-miR-197-3p, hsamiR-
513b-5p) and 7 mRNAs (CENPF, TOP2A, ARHGAP11A, CEP55, MELK, DTL, and
ANLN) were constructed. In vitro knockdown of LINC01094 expression in renal tubular epithelial
HK2 cells significantly attenuated the phenotype of TGFβ1-induced cell fibrosis.

Results:
The results of RNA immunoprecipitation (RIP) experiments and dual-luciferase reporter
experiments based on constructed mutants confirmed that LINC01094 could mediate
MELK expression by sponging miR-513b-5p.

Conclusion:
Our observations indicated that lowering the expression of LINC01094 can significantly
attenuate the TGFβ1-induced fibrosis phenotype in HK2 cells and renal inflammation
through the miR-513b-5p/MELK/Smad3 signalling axis.
Intra-articular Corticosteroid Injection for Adhesive Capsulitis: A Randomized Controlled Trial
Authors: Chan Julie , Tucker Allison , Hiscox Christina , Fenton Paul , Bicknell Ryan T 
Published: September 2024

Background
Adhesive capsulitis is a common cause of glenohumeral joint pain and stiffness that significantly interferes with patients' lives and ability to work. Since no specific treatment options have been well established, this research examines arthrographic injection of the glenohumeral joint with steroid and local anesthetic versus local anesthetic alone for the treatment of adhesive capsulitis.

Methods
A double-blinded randomized controlled trial was conducted with patients diagnosed with idiopathic adhesive capsulitis. They were randomized to one of two groups: (1)local anesthetic plus steroid or (2)local anesthetic. A musculoskeletal radiologist performed all of the image-guided injections using a standardized patient-blinded technique. Outcome measures included shoulder range of motion, grip strength, and scores on the Shoulder Pain and Disability Index (SPADI), Constant Score, and Visual Analog Scale (VAS) for pain. Evaluations occurred at baseline and 3-, 6- and 12-weeks post-injection.

Results
Thirty-seven patients were enrolled in the study. There was no significant improvement in shoulder range of motion and grip strength with local anesthetic plus corticosteroid injection compared to local anesthetic alone. Shoulder range of motion was better at all time points for the group with local anesthetic alone. Both groups showed significant improvements in their VAS and SPADI pain scores at all post-injection follow-up appointments (p=.011). The SPADI results showed significantly better disability scores for the local anesthetic plus steroid group (p=.012) and improved pain scores from baseline at all follow-up times (p=.011), whereas the local anesthetic group had significant pain relief for only the first 3 weeks (p=.050).

Conclusion
Intra-articular injection with local anesthetic plus steroid was beneficial in improving pain but not range of motion when compared to injection with local anesthetic alone.
The Influence of Some Environmental Factors on Temporary Degradation of Organic Components of Paper Pulp and Writing Compositions
Authors: Mehrabova M.A. , Gulmemmedov K. J. , Kochemirovskaya S.V. , Kozhakhmetov S.K. , Barova V.O. , Mokhorov D.D. , Kochemirovsky V.A. 
Published: September 2024

Abstract:
Paper sheet materials and written compositions are complex multi-component mixtures,
including dyes, solvents and resins, preservatives, and antioxidants, i.e., complex organic mixtures
that change over time. Technical examination of materials and documents is in demand in the judicial
and archival industry. Determining the actual date of appearance of a particular inscription on a
sheet of paper is one of the most important and complex expert tasks. The task is divided into two
components: analysis of the processes of temporary degradation of paper pulp and processes of temporary
degradation of organic writing materials.
Phytochemical Screening of Genus Primula Species Growing in Georgia and Study of their Antioxidant and Anti-inflammatory Potentials
Authors: Sukhishvili Nino , Franck Thierry , Mouithys-Mickalad Ange , Frederich Michel , Mulkijanyan Karen , Sandersen Charlotte , Serteyn Didier , Mskhiladze Lasha 
Published: September 2024

Background:
In traditional medicine, species of the genus Primula L. are used to treat
various health conditions, such as eye disorders, respiratory infections, headaches, epilepsy, insomnia,
as expectorants, and to promote wound healing.

Objective:
The goal of this article was to evaluate the antioxidant and anti-inflammatory activities of
three species from genus Primula L. growing in Georgia: Primula macrocalyx, Primula woronowii
and Primula saguramica.

Methods:
Initially, fractions containing both aerial and underground parts were air-dried, ground,
and extracted with 80% ethanol. The extract was then concentrated by condensation and further dried
through freeze-drying. Subsequently, additional chromatographic separations were carried out on
Diaion HP-20 using solvents such as water, methanol (50% and 100%), and 100% ethyl acetate to
isolate the desired fractions. To identify flavonoids and triterpene glycosides, the study employed
thin-layer chromatography (TLC) alongside preliminary phytochemical tests. The antioxidant activity
of these species was estimated in vitro by cell-free systems using ABTS and DPPH assays. The
extracts' anti-inflammatory properties were evaluated using an ex-vivo cell system that isolated neutrophils.
The study examined the extracts' impact on reactive oxygen species (ROS) production in
neutrophils stimulated with PMA, as well as their effect on the catalytic activity of myeloperoxidase
(MPO), a marker of inflammation in neutrophils.

Results:
Flavonoids and triterpene glycosides were primarily identified in the 50% and 100% methanol
(MeOH) fractions of Primula species through TLC and preliminary phytochemical tests. For each
experiment, gallic acid and quercetin served as standards at a concentration of 1 mg/ml, while the
tested samples were prepared at concentrations of 5 mg/ml. Based on the IC50 findings, P. w 3 exhibits
the most potent antioxidant and anti-inflammatory properties, as evidenced by the following
indicators: ABTS - IC50=8.51 ± 0.18; DPPH - IC50=34.57 ± 0.47; PMN - IC50=0.68 ± 0.04; SIEFED
- IC50=1.49 ± 0.7; and classical IC50=1.89 ± 0.01.

Conclusion:
Among the tested fractions, only the fraction of the 3 species prepared in MeOH (50%)
showed the best dose-dependent antioxidant and anti-inflammatory activities, especially P.w 3 which
is probably related to the high flavonoid content found in this species.