bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous glucose monitoring using machine learning models and IoT device data: A meta-analysis
Authors: Kapoor Yagyesh , Hasija Yasha 
Published: September 2024

BACKGROUND: Machine learning offers diverse options for effectively managing blood glucose levels in diabetes patients. Selecting the right ML algorithm is critical given the array of available choices. Integrating data from IoT devices presents promising opportunities to enhance real-time blood glucose management models. OBJECTIVE: This meta-analysis aims to evaluate the effectiveness of machine learning models utilizing IoT device data for predicting blood glucose levels. METHODS: We systematically searched electronic databases for studies published between 2019 and 2023. We excluded studies lacking ML model derivation or performance metrics. The Quality Assessment of Diagnostic Accuracy Studies tool assessed study quality. Our primary outcomes compared ML models for BG level prediction across different prediction horizons (PHs). RESULTS: We analyzed ten eligible studies across prediction horizons of 15, 30, 45, and 60 minutes. ML models exhibited mean absolute RMSE values of 15.02 (SD 1.45), 21.488 (SD 2.92), 30.094 (SD 3.245), and 35.89 (SD 6.4) mg/dL, respectively. Random Forest demonstrated superior performance across these PHs. CONCLUSION: We observed significant heterogeneity across all subgroups, indicating diverse sources of variability. As the PH lengthened, the RMSE for blood glucose prediction by the ML model increased, with Random Forest showing the highest relative performance among the ML models.
Echinacoside inhibits hepatocellular carcinoma progression by targeting the miR-30c-5p/FOXD1/KLF12 axis
Authors: Wang Guoyu , Han Yang , Zhuang Juhua , Mai Zhongchao , Xia Wei , Ye Ying 
Published: September 2024

BACKGROUND: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-attributed mortality and the primary liver malignancy in the world. Echinacoside is a phenylethanoid glycoside derived from traditional Chinese medicinal herbs which possessed multiple health benefits on humans, including anti-tumor effects. OBJECTIVE: This study aimed to demonstrate the function of echinacoside in HCC progression and the involvement of miR-30c-5p/FOXD1/KLF12 axis. METHODS: The HepG2 cells were treated by different dose of echinacoside, miR-30c-5p mimic, miR-30c-5p inhibitor, and FOXD1 overexpression lentiviruses or siRNA individually or simultaneously. The cell invasion and migration were measured by transwell assay. RNA and protein levels were tested by RT-PCR and western blot, respectively. The regulatory function of miR-30c-5p on Forkhead box D1 (FOXD1), FOXD1 on Krüppel-like factor 12 (KLF12) was tested by luciferase reporter assay or/and ChIP assay. Meanwhile, a liver cancer lung metastasis mice model was used to examine the functions of echinacoside and miR-30c-5p on HCC metastasis in vivo. Moreover, the correlations among miR-30c-5p, FOXD1, KLF12, and HCC prognosis was analyzed using clinical sample and TCGA database. RESULTS: Based on both in vitro and in vivo investigations, we found that echinacoside could inhibit HCC cell migration, invasiveness, and tumor metastasis, and associated with the enhanced miR-30c-5p/FOXD1/KLF12 axis. Furthermore, through analyzing the interactions among intermediate molecules, we revealed that miR-30c-5p, FOXD1, and KLF12üere clinically relevant with each other in HCC patients, correlated with HCC prognosis, and regulated by echinacoside to contribute in the inhibition of HCC progression. CONCLUSIONS: These findings suggest that echinacoside could inhibit HCC progression, and the mechanism related to the enhanced miR-30c-5p/FOXD1/KLF12 axis. Moreover, the abovementioned intermediate molecules might serve as prospective biomarkers for HCC prognosis.
Platinum(II) complexes based on derivatives of natural chlorins with pyridine-containing chelate groups as prototypes of drugs for combination therapy in oncology
Authors: Kirin N. S. , Ostroverkhov P. V. , Usachev M. N. , Birin K. P. , Grin M. A. 
Published: September 2024

Objectives. To synthesize Pt-containing derivatives of natural chlorins as potential agents for the combination therapy in oncology. Platinum compounds are known to occupy an important place as chemotherapeutic agents in the treatment of oncological diseases. However, Pt(II) complexes are highly toxic to the body and are not selectively accumulated in tumor cells. If photodynamic and chemotherapy methods are combined in a single drug, the pigments are responsible for the selectivity of conjugate accumulation in the tumor, while a chemotherapeutic agent based on Pt(II) complexes is responsible for the cytotoxic effect on tumor cells. This will not affect healthy cells and thereby minimize the systemic toxicity of the drug to the body.Methods. Methods for the synthesis of pyridine-containing derivatives of natural chlorins and their metal complexes for use as potential binary agents in oncology were applied. As part of the study, the structures of the compounds obtained were confirmed by mass spectrometry, nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and high-resolution chromatography-mass spectrometry. Preparative methods, including thin-layer and column chromatography, centrifugation and recrystallization, were used to isolate and purify the compounds obtained.Results. Platinum(II) complexes of pyridine-containing derivatives of natural chlorins were obtained for application in combination therapy in oncology. The schemes for synthesizing the target photosensitizers were optimized, in order to increase the yields and for subsequent transfer to industrial sites.Conclusions. It was found that pyridine-containing derivatives of natural chlorins could be obtained in high yields, that they possess chelating properties for platinum, and can be considered as binary agents in cancer therapy after successful preclinical trials.
N-[(1RS)-Camphan-2-ylidene]-4-ethoxyaniline and its reduction product as stabilizers of nitrile butadiene rubbers
Authors: Nilidin D. A. , Vaniev M. A. , Vernigora A. A. , Davidenko A. V. , Salykin N. A. , Minh Thuy Dang , Gubin S. G. , Novakov I. A. 
Published: September 2024

Objectives. To investigate the protective efficacy of unreduced and reduced forms of the condensation product of D,L-camphor and p-ethoxyaniline in nitrile butadiene rubber formulations when compared with the conventional stabilizer N-isopropyl-N′-phenyl- p-phenylenediamine aged under laboratory and in situ climatic conditions in the tropics.Methods. The thermostabilizing effect of the condensation product of D,L-camphor and p-ethoxyaniline was evaluated by means of infrared spectroscopy using the dynamics of changes in the absorption bands of carbonyl and hydroxyl groups. The features of rubber vulcanization were studied by means of rotorless rheometry. Changes in the physical and mechanical properties of rubbers and degree of cross-linking were evaluated after thermo-oxidative aging in laboratory conditions. They also took into account the results of longterm exposure of samples in undeformed and deformed state in a tropical climate, taking into account meteorological data of the Can Gio climatic testing station.Results. It was found for the first time that condensation products of D,L-camphor and p-ethoxyaniline exhibit a stabilizing effect in formulations of rubbers based on polar nitrile butadiene rubber.Conclusions. According to the results of laboratory and in situ tests, the study established that the use of N-[(1RS,2RS)-camphan-2-yl]- 4-ethoxyaniline (reduced form) as an antifatigue agent is preferable. This is due to the presence of a mobile hydrogen atom at the nitrogen atom. The protective effect is manifested in terms of the better preservation of elastic-strength properties of rubbers with less change in hardness.
Calculation Analysis of VVER‑1000 RCCA Elements to Justify Lifetime Extension
Authors: Makarenko А. , Chaikovskyi M. , Mazurok O. , Zuyok V. , Mykhaylenko O. 
Published: September 2024

This paper presents the main results of strength calculations to justify the integrity of structural elements of the control rods and rod cluster control assemblies under static and dynamic loads in various (all possible) operating modes of rod cluster control assemblies, with the mechanical properties of the material after neutron irradiation, in order to lifetime extend their lifetime.
The presented results of calculations of the stress-strain state of rod cluster control assembly elements indicate that the strength conditions are met under the operating modes considered in this paper (including the maximum design basis earthquake). The maximum stresses occur in the joint zone of the cladding with the upper end element, and the corresponding minimum value of the strength factor is 1.04. The obtained stress values in the control rods and the rod cluster control assembly spider do not exceed the yield strength of the material (RT0.2 = 245 MPa and RT0.2 = 167 MPa, respectively), which does not result in residual deformations.
The results of the cyclic strength calculation show that the requirements for cyclic strength are met, the maximum predicted damage is only 5.82 × 10-02 for the control rods and 4.27 × 10-07 for the spider. Consequently, this guarantees the structural integrity of the control rods and rod cluster control assemblies at their extended lifetime.
The safe operation (lifetime) of the rod cluster control assemblies currently loaded in VVER‑1000 reactors at Ukrainian NPPs was extended basing on the above justifications and positive results of strength calculations, assuming that fast neutron fluence (E > 0.1 MeV) does not exceed 3.4 × 1022 n/cm2, which, according to the previously performed neutron calculations, corresponds to the lifetime in the manual and automatic control groups up to 38,000 effective hours, in reactor scram groups – approximately up to 113,500 effective hours.
Molecular Epidemiology of mcr-1-Positive Polymyxin B-Resistant Escherichia coli Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China
Authors: Liu Yue , Wang Qian , Qi Ting , Zhang Meng , Chen Ran , Si Zaifeng , Li Jinmei , Jin Yan , Xu Qingbing , Li Ping , Hao Yingying 
Published: September 2024

Abstract

Escherichia coli, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing mcr-1 -positive E. coli in Shandong, China. We collected 668 non-duplicate ESBL-producing E. coli strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK® 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the mcr-1 gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the mcr-1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene mcr-1, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the mcr-1 gene into E. coli J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene mcr-1. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no ISApl1 insertion sequences were found on either side of the mcr-1 gene. Our study uncovered the molecular epidemiology of mcr-1-carrying ESBL-producing E. coli in the region and suggested horizontal transmission mediated by plasmids as the main mode of mcr-1 transmission.
Акместратегія та акмеологічні технології розвитку професіоналізму та кар’єрного зростання педагогічного працівника закладу освіти
Authors: Гладкова Валентина 
Published: September 2024

У статті аналізується сутність понять «акмеологічна стратегія» та «акмеологічна технологія». Ці поняття розглядаються в розрізі розвитку/саморозвитку педагогічного працівника закладу освіти. Підкреслюється важливість визначення та розробки заходів щодо горизонтального та вертикального просування працівника в системі посад освітньої організації.
У процесі стратегічного планування кар’єрного росту будь-якого педагогічного працівника закладу освіти необхідно враховувати акме-синергетичні підхід і закон. Наведена структура акмеологічої моделі стратегії індивідуального кар’єрного розвитку, що містить 4 блоки: вивчення кар’єрного розвитку за допомоги показників і рівнів для кожного критерію оптимізації кар’єрного розвитку; акмеологічні умови і фактори, що сприяють кар’єрному розвиткові; алгоритм індивідуальної стратегії кар’єрного зростання; програма психолого-акмеологічного супроводу оптимізації індивідуальної стратегії кар’єрного розвитку.
Здійснена класифікація цілей кар’єрного розвитку у професійній діяльності педагогічного працівника закладу освіти. Сформована стратегічна система ціннісно-значущих цілей кар’єрного розвитку. Беручи за методологічну підставу акмесинергетичний підхід змодельовано алгоритм шляху кар’єрного розвитку педагогічного працівника закладу освіти.
Реалізація акместратегій у професійній діяльності педагога закладу освіти здійснюється за допомогою акметехнологій. Завдання акмеологічних технологій професійного розвитку сучасного педагогічного працівника закладу освіти полягає у формуванні та закріпленні в його свідомості необхідності в самопізнанні, саморозвиткові, самовдосконаленні та самореалізації, що уможливлюють з використанням спеціальних прийомів і технік самоактуалізацію особистісного та професійного «Я». До таких прийомів належать: індивідуальне консультування; акмеологічна та психодіагностика; акмеограми, індивідуальні плани (траєкторії) професійного розвитку; тест-тренажери; акмеологічний тренінг тощо.
Результат застосування акметехнологій педагогічним працівником освітнього закладу визначається ступенем розвиненості соціально-професійного та особистісного мислення; сформованістю особистісно-професійної позиції педагога; розвиненістю відповідальної громадянської і соціальної позиції особистості; готовністю до безперервного саморозвитку.
Mendelian randomization analysis reveals no causal relationship between thyroid function and sarcopenia-related traits
Authors: Xu Rui , Li Yan-Yan , Xu Hong 
Published: September 2024

BackgroundRecent research has indicated a potential association between thyroid function and sarcopenia, but the specific mechanisms and a definitive causal relationship have yet to be established. Therefore, the objective of this study is to examine the potential causal connection between thyroid function and sarcopenia-related traits, including hand-grip strength, appendicular lean mass (ALM), and walking pace.MethodsThe study used a bi-directional two-sample MR design, with thyroid function examined as the exposure and sarcopenia-related traits as the outcome in the first stage, and then reversed in the second stage. The genetic instruments for thyroid function were obtained from a comprehensive meta-analysis involving 271,040 participants. Data on sarcopenia-related traits based on GWASs were collected from the UK Biobank, which includes up to 461,026 European participants. The estimates for MR were calculated using the inverse-variance weighted (IVW) method, and several sensitivity analyses were performed.ResultsAfter applying the Bonferroni correction for multiple testing, our MR analyses revealed no significant impact of thyroid function liability on sarcopenia-related traits. Similarly, our reverse MR analysis did not provide evidence supporting the influence of liability to sarcopenia-related traits on thyroid function. The results of the primary IVW MR analyses were largely in line with those obtained from our sensitivity MR analyses.ConclusionOur research findings do not suggest a link between thyroid function and sarcopenia-related traits. The associations identified in epidemiological studies may be influenced, at least in part, by shared biological mechanisms or environmental confounders.
Poor prognostic factors for relapse of interstitial lung disease with anti-aminoacyl-tRNA synthetase antibodies after combination therapy
Authors: Matsuda Shogo , Kotani Takuya , Oe Katsumasa , Okazaki Ayana , Kiboshi Takao , Suzuka Takayasu , Wada Yumiko , Shoda Takeshi , Takeuchi Tohru 
Published: September 2024

IntroductionThis study aimed to identify useful clinical indicators for predicting the relapse of interstitial lung disease (ILD) complicated with anti-aminoacyl-tRNA synthetase (ARS) antibodies (anti-ARS-ILD), being treated with prednisolone and calcineurin inhibitors.MethodsFifty patients with anti-ARS-ILD were enrolled between October 2014 and August 2022. All patients were treated with prednisolone and calcineurin inhibitors as remission induction therapy and followed up for over a year with these combination therapies. We examined patients who experienced ILD relapse after immunosuppressive treatment. We explored the risk factors for predicting ILD relapse in these patients by comparing demographic, clinical, laboratory, and radiological findings and treatments between the relapsed and non-relapsed groups on admission.ResultsOf the 50 patients, 19 (38%) relapsed during a median follow-up of 4.8 years. Univariate and multivariate Cox regression analyses identified the presence of acute/subacute (A/S)-ILD, higher serum aldolase (ALD) and surfactant protein-D (SP-D) levels, and lower %forced vital capacity (FVC) as risk factors for relapse in patients with anti-ARS-ILD. Using the receiver operating curve analysis, ALD ≥6.3 U/L, SP-D ≥207 ng/mL, and %FVC ≤76.8% were determined as the cut-off levels for indicating a poor prognosis. The 5-year relapse rate was significantly higher in patients with A/S-ILD, serum ALD≥6.3 U/L, serum SP-D ≥207 ng/mL, or %FVC of ≤76.8% than in those without these parameters. (P=0.009, 0.0005, 0.0007, 0.0004, respectively) Serum ALD levels were significantly correlated with the disease activity indicators of anti-ARS-ILD.ConclusionThe presence of A/S-ILD, higher serum ALD and SP-D levels, and lower %FVC are useful indicators for predicting anti-ARS-ILD relapse.
The role of parental characteristics, home language use, and schooling in children's Mandarin heritage language development in Canada
Authors: Daskalaki Evangelia , Soto-Corominas Adriana , Xia Vera Yunxiao , Paradis Johanne 
Published: September 2024

IntroductionThis study on child HL (heritage language) speakers of Mandarin examines the associations between parental characteristics (attitudes and proficiency), children's HL use (at home and through schooling), and children's HL outcomes (in vocabulary and simple syntax).MethodsForty-seven Mandarin-English bilingual children of Chinese heritage (mean age: 10.5; age range: 6.8–16.2) residing in Western Canada participated in the study. All children were second-generation immigrants, and received one of three types of schooling. There were 11 children who attended English-only schools (English school group), 21 who attended English-only schools but also after-school heritage Mandarin classes (Heritage school group), and 15 who attended English-Mandarin bilingual schools (Bilingual school group). The children were administered two tasks: a picture-naming task targeting Mandarin vocabulary (LITMUS-CLT) and an experimental elicitation task targeting Mandarin wh-interrogative sentences (an early-acquired structure). Parents were administered a questionnaire about home language environment, attitudes toward Mandarin transmission, and their children's schooling choices.ResultsResults showed that positive parental attitudes and lower parental proficiency in English were associated with more Mandarin use at home. More Mandarin use at home, in turn, was associated with larger vocabularies and more accurate production of interrogatives. By contrast, school type was only associated with vocabulary and not syntax: the Bilingual school group had larger vocabularies than the English school group.DiscussionOverall, these results show how parental characteristics may influence input factors, which in turn may differentially affect the acquisition of vocabulary vs. early-acquired syntactic structures.
Endogenous orexin and hyperacute autonomic responses after resuscitation in a preclinical model of cardiac arrest
Authors: Guo Yu , Gharibani Payam , Agarwal Prachi , Modi Hiren , Cho Sung-Min , Thakor Nitish V. , Geocadin Romergryko G. 
Published: September 2024

ObjectivesThe study of autonomic responses to cardiac arrest (CA) resuscitation deserves attention due to the impact of autonomic function on survival and arousal. Orexins are known to modulate autonomic function, but the role of endogenous orexin in hyperacute recovery of autonomic function post-resuscitation is not well understood. We hypothesized that endogenous orexin facilitates hyperacute cardiovascular sympathetic activity post-resuscitation, and this response could be attenuated by suvorexant, a dual orexin receptor antagonist.MethodsA well-established 7-min asphyxial CA rat model was studied. Heart rate (HR) and blood pressure were monitored from baseline to 90-min post-resuscitation. Autonomic function was evaluated by spectral analysis of HR variability, whereby the ratio of low- and high-frequency components (LF/HF ratio) represents the balance between sympathetic/parasympathetic activities. Plasma orexin-A levels and orexin receptors immunoreactivity in the rostral ventrolateral medulla (RVLM), the key central region for regulating sympathetic output, were measured post-resuscitation. Neurological outcome was assessed via neurologic-deficit score at 4-h post-resuscitation.Key resultsA significant increase in HR was found over 25–40 min post-resuscitation (p &lt; 0.01 vs. baseline), which was attenuated by suvorexant significantly (p &lt; 0.05). Increased HR (from 15-to 25-min post-resuscitation) was correlated with better neurological outcomes (rs = 0.827, p = 0.005). There was no evident increase in mean arterial pressure over 25–40 min post-resuscitation, while systolic pressure was reduced greatly by suvorexant (p &lt; 0.05). The LF/HF ratio was higher in animals with favorable outcomes than in animals injected with suvorexant over 30–40 min post-resuscitation (p &lt; 0.05). Plasma orexin-A levels elevated at 15-min and peaked at 30-min post-resuscitation (p &lt; 0.01 vs. baseline). Activated orexin receptors-immunoreactive neurons were found co-stained with tyrosine hydroxylase-immunopositive cells in the RVLM at 2-h post-resuscitation.ConclusionTogether, increased HR and elevated LF/HF ratio indicative of sympathetic arousal during a critical window (25–40 min) post-resuscitation are observed in animals with favorable outcomes. The orexin system appears to facilitate this hyperacute autonomic response post-CA.