bluuebunny/crossref_metadata_2025_split huggingface dataset:
Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
Authors: Parker Matthew , Hillman Courtney , Fontana Barbara , Amstislavskaya Tamara , Gorbunova Maria , Altenhofen Stefani , Barthelson Karissa , Bastos Leonardo , Borba João , Bonan Carla , Brennan Caroline , Farias-Cea Amaury , Cooper Austin , Corcoran Jamie , Dondossola Eduardo , Martinez-Duran Luis , Gallas-Lopes Matheus , Galstyan David , Garcia Ella , Gerken Ewan , Hindges Robert , Kenney Justin , Kleshchev Maxim , Kolesnikov Tatiana , Leggieri Adele , Khatsko Sergey , Lardelli Michael , Lodetti Guilherme , Lombardelli Giulia , Luchiari Ana , Portela Stefani , Medan Violeta , Moutinho Lirane , Nekhoroshev Evgeny , Petersen Barbara , Petrunich-Rutherford Maureen , Piato Angelo , Porfiri Maurizio , Read Emily , Resmim Cássio , Rico Eduardo , Rosemberg Denis , de Abreu Murilo Sander , Salazar Catia , Stahloher-Buss Thaliana , Teixeira Júlia , Valentim Ana , Zhdanov Alexander , Iturriaga-Vásquez Patricio , Wang Xian , Wong Ryan , Kalueff Allan 
Published: October 2024

Abstract

The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
Psychosocial, Physical problems and Coping strategies of Patients having Long term / Permanent Tracheostomy.
Authors: Negi Monika , M.S Cecilia , sharma Kamlesh Kumari , Thakar Alok , Srivas Saumya P. 
Published: October 2024

Abstract

Background
Tracheostomy is a common surgical procedure in which a stoma is created in trachea by direct incision over the anterior aspect of the neck, which can cause disfigurement of the anterior neck and many other associated physical and psychosocial issues. Patients with long-term/permanent tracheostomy have to live with tracheostomy even after getting discharged from the hospital, which can further complicate the condition of the patients as they are unable to cope up with the problems associated with long-term tracheostomy. There is dearth of literature related to intensity of problems among patients having long term/ permanent tracheostomy in India. Therefore, the aim of the study is to assess psychosocial, physical problems and coping mechanism in patients with long-term/permanent tracheostomy.
Methods
A cross-sectional descriptive study was conducted at ENT OPD and ENT Minor OT, tertiary care hospital India. Total of 100 patients having long term/permanent tracheostomy were enrolled by convenient sampling. Data regarding demographics and clinical characteristics, Physical and Psychosocial problems, and Coping strategies were collected by structured questionnaires.
Result
The majority of individuals who had long-term or permanent tracheostomies had only minor health issues. Patients experienced more psychosocial issues than people in the general healthy population. The majority of patients employed active coping, acceptance, and religion as coping mechanisms, whereas the least number of patients used humor and substance abuse. Patients' coping strategies vary significantly according on their age, employment status, marital status, and the duration of their tracheostomy.
Conclusion
Long-term/Permanent tracheostomy can cause multiple physical and psychosocial issues in patients, but eventually, patients get adapted to it. Support from family members and significant others plays a vital role in better coping with tracheostomy in situ, also it is critical for health care providers to be aware of their problems and coping mechanisms so that they can provide required care and assistance.
Diagnostic value of dual-energy CT in detecting irreversible transmural intestinal necrosis in patients with acute occlusive mesenteric ischemia
Authors: Yang Jushun , Xu Zhenyu , Chen Feixiang , Wang Meirong , Fan Xiaole , He Bosheng 
Published: October 2024

Abstract

Background
Irreversible transmural intestinal necrosis (ITIN) is associated with high mortality rates in patients with acute occlusive mesenteric ischemia (AOMI). However, the important radiographic predictors of ITIN remain unknown. This study evaluated the diagnostic value of dual-energy CT (DECT) for ITIN in AOMI.
Methods
The cases and CT images of 102 patients with clinically diagnosed AOMI (including 48 ITIN) from January 2012 to January 2022 were retrospectively collected. The CT scans included both MDCT and DECT. Two radiologists independently completed the subjective visual assessment of CT signs related to AOMI. DECT scans the portal vein phase raw data to reconstruct a 120 kVp mixed energy image, 50 keV virtual monoenergetic imaging (VMI), and iodine map. Objective parameters, including CT values (CT 50 keV lesion, CT 50 keV normal/lesion) and iodine concentrations (IC lesion and IC normal/lesion), were quantified. Furthermore, multivariate logistic regression, receiver operating characteristic (ROC) curves, and area under the curve (AUC) values were used to evaluate the subjective/objective indicators in predicting ITIN.
Results
Regarding subjective signs, logistic regression analysis revealed reduced or absent bowel wall enhancement (odds ratio [OR] = 5.576, 95% confidence interval [CI]: 1.547–20.093), bowel dilation (OR = 11.613, 95% CI: 3.790–35.586), and parenchymatous organ infarction (OR = 4.727, 95% CI: 1.536–14.551) were independent risk factors for the ITIN. CT subjective signs had a high diagnostic efficacy for ITIN (AUC = 0.853). The two DECT objective parameters also exhibited excellent diagnostic value for ITIN, with an AUC of 0.79, a CT cutoff value of CT 50keV normal/lesion = 2.81, and an AUC of 0.777 with a cut-off value of IC normal/lesion = 2.39. Importantly, the Delong test showed that there was no significant difference in the efficacy of subjective CT signs and objective DECT parameters (p > 0.05), indicating that the two objective parameters could replace CT signs in predicting ITIN. Furthermore, we observed that IC normal/lesion combined with subjective signs (bowel dilation and parenchymatous organ infarction) had the highest predictive performance (AUC = 0.896), sensitivity (100%), and specificity (70.83%).
Conclusion
IC normal/lesion (DECT-based features) combined with CT signs showed favorable predictive performance for ITIN in AOMI, which may help clinicians develop timely treatment strategies.
Comparison of a single- and dual-arm collaborative robots used for precision assembly
Authors: Peta Katarzyna , Wiśniewski Marcin , Kotarski Mikołaj , Ciszak Olaf 
Published: October 2024

Abstract

The aim of these studies is to evaluate ABB YuMi collaborative robots in single- and dual-arm variants for precise assembly of a computer motherboard. The characteristics of the object intended for assembly and the RobotStudio environment are presented. The tool path, assembly times, accelerations and speeds of the robot arms were analyzed, as well as cost estimation and energy consumption. A multi-criteria comparative evaluation of collaborative robots can allow for their accurate selection in industrial applications. The presented simulation tests and analyzes can be used at the initial design stage of robotic cell for the assembly of selected components of the PC motherboard.
The Genomic Medicine for Everyone (Geno4ME) Study: Implementation of Whole Genome Sequencing for Population Screening in a Large Healthcare System
Authors: Gordon Ora , Beckett Isabelle Lucas , Emery Kate , Wagner Josiah , Jade Kathleen , Cosgrove Benjamin , Welle John , Rinaldi JB , Dickey Lindsay , Jones Kyle , Wagner Nick , Shull Eric , Clemens Jon , Trachana Kalliopi , Anderson Lance , Kudla Allison , Smith Brett , Verespej Hakon , Yuan Li Chi , Denne Elizabeth , Ramos Erica , Lovejoy Jennifer , Olszewski Krzysztof , Reese Martin , Reynolds Misty , Miller Mandy , Dowdell Alexa , Beck Brianna , Campbell Mary , Magis Andrew , Vartanian Keri , Piening Brian , Bifulco Carlo 
Published: October 2024

Abstract

Population-scale genomics programs may enable increased access to genomic medicine. The Genomic Medicine for Everyone (Geno4ME) program was established across the diverse seven-state Providence Health system to enable genomics research and genome-guided care pathways across patients’ lifetimes. Key components included targeted and multi-lingual outreach to underrepresented groups, a novel electronic informed consent (e-consent) and education platform, and whole genome sequencing (WGS) with clinical return of results and integration into the electronic health record (EHR) for 78 hereditary disease genes and four pharmacogenes. Clinical whole genome sequences were banked for research, programmatic expansion of returnable results, and variant reanalysis. The program provided genetic counseling, pharmacist support, and guideline-based clinical recommendations for patients and their providers. During the two years of the study, over 30,800 potential participants were contacted; out of these, 2,716 were consented to the study (of which 47.5% were people of color) and 2,017 had results returned. One hundred fifty-eight (7.8%) participants had an actionable gene variant in the hereditary disease panel, 294 (14.6%) of participants had a pharmacogenomic (PGx) recommendation for one or more of the supported medications reported at time of enrollment, and overall, 21.4% of participants had a test result with at least one medical intervention recommendation. Future work will involve strategies to maintain engagement and education around genomic medicine. We propose the Geno4ME model as a framework to integrate population health genomics into routine healthcare and present lessons learned that may aid in the design of future programs.
Association of MRI markers of cerebral small vessel disease and ischemic stroke recurrence in patients treated with intravenous thrombolysis: A three-year prospective cohort study
Authors: Liu Xue-yun , Wu Xiao-san , Yan Sun-hong , Chen Chu , Wang Long , Li Huan , Zhu Lei , Fang Chuan-qin , Tian Yang-hua , Fang Qi , Li Qi 
Published: October 2024

Abstract

Background and Objectives:
It remains unclear whether the total cerebral small vessel diseases (CSVD) burden can predict risk of recurrent ischemic stroke in patients treated with intravenous thrombolysis (IVT). We aim to investigate the association between the total CSVD burden and recurrent ischemic stroke in patients treated with IVT.
Methods
We enrolled patients with acute ischemic stroke (AIS) undergoing IVT between September 2017 and June 2019. According to the total burden rating scale of CSVD on MRI, we calculated the total CSVD burden score. Study participants were followed up regularly by two neurologists at 3,6,12,24 and 36 months after the index AIS onset. We used Kaplan–Meier survival curves and Cox proportional hazards regression models to attempt to prove the associations between the total CSVD burden and recurrent ischemic stroke.
Results
We found that recurrent ischemic stroke events in patients treated with IVT were associated with total CSVD burden, higher cumulative incidence rates of recurrent stroke were more common in higher CSVD score (log-rank P ≤ 0.001). In multivariate Cox regression analysis, diabetes (adjusted HR = 4.065,95%CI = 2.143–7.713, P = 0.000), secondary prevention (adjusted HR = 0.322,95%CI = 0.150–0.691, P = 0.004), and the total CSVD score of 3 point (adjusted HR = 4.907,95%CI = 1.017–23.678, P = 0.048), 4 point (adjusted HR = 13.480,95%CI = 2.587–70.245, P = 0.002) were independently associated with recurrent ischemic strokes within 3 years. However, the total CSVD score of 1 point (adjusted HR = 1.524,95%CI = 0.313–7.412, P = 0.602), 2 point (adjusted HR = 3.111,95%CI = 0.668–14.495, P = 0.148) were not associated with recurrent ischemic stroke. Increasing CSVD score was also associated with all-cause mortality and ischemic stroke events mortality during follow-up in patients treated with IVT.
Conclusions
Total CSVD burden was associated with recurrent ischemic stroke in patients treated with IVT. Most notably the total CSVD score of 3 or 4 resulted in a more than 4-fold increase in risk of recurrent ischemic stroke in patients treated with IVT.
We should pay more attention to the complexity of the intrinsic entanglement between GPSM2 and HCC
Authors: Li Sijin , Ren Biqiong 
Published: October 2024

Abstract

Overexpression of G-protein signaling modulator 2 (GPSM2) is a common feature of many cancers, however, detailed research regarding the role of GPSM2 in the immune microenvironment of hepatocellular carcinoma (HCC) is lacking. In this study, databases were utilized to analyze the expression, the relationship between GPSM2 and immune infiltration, and the effect of GPSM2 on the prognosis of patients with HCC. The results show that GPSM2 was associated with oncogenes KRAS, FGFR2, PI3KCA, and AKT1, as well as with CDK1/2/4 and E2F1, which are enriched in the cell cycle. A positive correlation with apoptosis inhibitory protein BCL2, AASDH, GLIS2, GNAI1, GNAI2, NUMA1, and GNAO1 was observed, along with CTLA4, CD274, HAVCR2, LAG3, PDCD1, PDCD1LG2, TIGIT, SIGLEC15, and PSMG1. Alterations in copy number and DNA methylation levels could be the underlying mechanisms responsible for GPSM2 regulation. has-miR-22-3p might be the most potential regulatory miRNA of GPSM2, and BX284668.6, LINC00858, and MIR4435-2HG might be the three most upstream lncRNAs of the has-miR-22-3p/GPSM2 axis in HCC. In summary, GPSM2 affects the tumor immune microenvironment, and targeting GPSM2 might increase the efficacy of immunotherapy in HCC. Alterations in copy number and DNA methylation levels could be the underlying mechanisms responsible for GPSM2 upregulation.
Predicting Individual Responses in Phase I Oncology Trials Using Routinely Collected Clinical Biomarkers
Authors: Bhadra Nivedita , Boyd Marley , Smith Sandra , Espirito Janet , Trent Jeffrey , Powell Christine , Koktavy Kati , Robert Nicholas , Frytak Jennifer , Goetz Laura H. , Sharma Sunil , Von Hoff Daniel D. , Schork Nicholas J. 
Published: October 2024

Abstract

Information which may support an individual’s participation in a cancer phase I trial, such as their response to prior therapies, other medical conditions they may have, features in their tumor genomic profile, etc., should be considered to avoid negative consequences of participating in the trial. However, knowing which pieces of information are relevant is crucial. We built predictive models of responses in phase I trials using routinely collected demographic and clinical chemistry data. We obtained data on 1386 participants in 252 phase I trials pursued within the US Oncology clinical trial network in the years 2007–2018. We used mixed models, as well as machine learning (ML) techniques exploiting randomly generated training and test data sets, to build predictive models of four different outcomes while controlling for which trial a participant was enrolled in. The outcomes of interest were time on the experimental therapy, time on the study drug relative to the drug during which their cancer progressed, 90-day mortality, and Grade 5 toxicity. We also controlled for other potential sources of variation in outcomes such as weight, height, and sex. We found that an unfavorable participant profile includes elevated white blood cells, low albumin levels, and low hemoglobin levels, as well as low BMI for mortality risk, among other factors, many of which are consistent with previously published findings. In addition, our ML-based predictions achieved, on average, > 80% area under the receiver/operator curve (AUC) statistics reflecting good accuracy for predicting dichotomous outcomes. Our findings could be of general use when recruiting for Phase I oncology clinical trials.
Incidence of bleeding and performance of the PRECISE-DAPT Score in predicting bleeding in patients on Dual Antiplatelet Therapy after treatment for acute coronary syndrome in Kenya
Authors: Mugo Peter , Ngunga Mzee , Jeilan Mohamed , Msunza Miriam , Nyaronga Beatrice , Biwott Petronilla , Njimu Charity 
Published: October 2024

Abstract

Introduction
Dual Antiplatelet Therapy (DAPT) plays an important role in the secondary prevention of ischemic events after treatment for acute coronary syndrome (ACS). The long-term use of DAPT is associated with an increased risk of bleeding, which affects morbidity and mortality. Risk stratification scores have been developed to predict this risk and provide a balance against the risk of ischemic events. The aim of this study was to determine the incidence of bleeding in a cohort of patients in Kenya on DAPT and assess the performance of the PRECISE-DAPT Score in predicting the risk of bleeding.
Methods
This was a retrospective cohort study conducted in three hospitals in Kenya among patients on DAPT after ACS between January 2019 and April 2022. We reviewed medical records for demographic and clinical characteristics and conducted telephone interviews to assess bleeding for patients on DAPT for a minimum period of one year. Bleeding events were categorized according to the TIMI criteria for bleeding, and the PRECISE-DAPT Score was calculated using an online calculator. The cumulative one-year incidence of bleeding was calculated and presented as frequencies and percentages. Receiver operating characteristic (ROC) analysis and C-statistics were used to quantify the ability of the PRECISE-DAPT Score to predict bleeding events, whereas calibration was estimated using the Hosmer‒Lemeshow goodness-of-fit test.
Results
A total of 202 patients were enrolled in the study. The study population was predominantly male (n = 156, 77.2%) and African (n = 141, 69.8%), with a median age of 61 years (IQR 52–72). Majority were admitted with ST-Elevation Myocardial infarction (STEMI) (n = 126, 62.4%) and had a mildly reduced left ventricle ejection fraction (n = 124, 61.4%). Hypertension (n = 94, 46.5%) was the most common comorbidity. Fourteen patients (6.9%) met the TIMI criteria for bleeding, of whom 11 (5.4%) had minimal bleeding and 3 (1.5%) had minor bleeding. There was no incidence of major bleeding. The discrimination and calibration of the PRECISE-DAPT Score was good {ROC curve 0.699 (95% CI: 0.564–0.835)} and the Hosmer–Lemeshow goodness-of-fit test (Chi-square, 6.53; p = 0.588), respectively.
Conclusion
The incidence of bleeding was low, with the majority of patients having minimal bleeding that did not require medical intervention. The PRECISE-DAPT Score performed well in predicting bleeding in patients on DAPT.
Memory-behavior correlation in pre-onset Alzheimer's reverses following beta amyloid accumulation
Authors: Wang Ziyi , Li Hui , Shi Bowen , Qin Qikai , Ye Qiong , Thompson Garth J. 
Published: October 2024

Abstract

Alzheimer’s disease (AD) causes cognitive decline with aging, hypothetically due to the accumulation of beta-amyloid (Aβ) plaques. Animal models are critical in the study of AD, and the 3xTg-AD mouse model is increasingly used due to its initial absence of significant physical or behavioral impairments in youth and progressive Aβ plaque development with age. This mouse model thus provides an opportunity to find early biomarkers for AD through two stages of study. However, while altered structural and functional networks occur across the whole brain in human AD, such whole-brain networks have never been studied changes in 3xTg-AD mice. Using wild-type (WT) and 3xTg-AD mice, aged 22 and 40 weeks (before and after Aβ plaque development), we measured resting state functional magnetic resonance imaging to examine functional connectivity (FC) between brain regions and diffusion tensor imaging to evaluate the structural connectivity (SC) and axonal integrity of brain white matter fiber bundles. At 22 weeks, 3xTg-AD mice unexpectedly had higher SC and FC, and there was positive correlation between behavioral performance and functional connectivity density. By 40 weeks, SC and FC was lower in AD mice (similar to human AD patients), but the behavior-functional correlation was negative. Thus, our novel methods identified a shift in 3xTg-AD mice between two abnormal states, with the latter state resembling human AD patients. Such a shift could be an early biomarker in human patients, or, if it is not present, that the 3xTg-AD mouse model only becomes relevant after this shift occurs.
Magnitude and Associated Factors of Selected Hematological and Coagulation Abnormalities among Renal Disease Patients at the University of Gondar Comprehensive Specialized Hospital Northwest Ethiopia
Authors: Workineh Mickael , Kassie Adamu , Sitotaw Chomaw , Bayleyegn Biruk , Birkie Bisrat , Woldu Berhanu 
Published: October 2024

Abstract

This study aimed to assess the magnitude and associated factors of selected hematological and coagulation abnormalities among renal disease patients at the University of Gondar Comprehensive Specialized Hospital Northwest Ethiopia from May to October 2023. An institutional-based cross-sectional study was conducted on 352 renal disease patients. Six mL of venous blood was collected for hematological and coagulation tests. STATA version 14.0 software was used for analyses. Both bi-variable and multivariable binary logistic regression analyses were done to determine associated factors for selected hematological and coagulation abnormalities. A p-value of < 0.05 was considered as statically significant. The overall magnitude of anemia, prolonged prothrombin time, and prolonged activated partial thromboplastin time were 59.38%, 22.24%, and 20.45%, respectively. Anemia was associated with female patients (AOR = 3.23, 95% CI: 1.46, 7.15), 46–64 years (AOR = 2.41, 95%CI: 1.01, 5.73), greater than 65 years (AOR = 3.41, 95% CI: 1.18, 9.81), lack of physical activity (AOR = 3.88, 95% CI: 1.50, 10.02). Prolonged prothrombin time was associated with female patients (AOR = 2.60; 95% CI: 1.45, 4.65), 46–64 years (AOR = 2.35 95% CI: 1.04, 5.32), greater than 65 years (AOR = 3.76 95% CI: 1.47, 9.61) and hypertension (AOR = 1.80 95% CI: 1.00, 3.24). Moreover, prolonged activated partial thromboplastin time was associated with greater than 65 years (AOR = 2.58: 95% CI: 1.17, 5.70), and lack of physical activity (AOR = 3.54: 95% CI: 1.32, 9.48). Hematological and coagulation abnormalities were prevalent among patients with renal disease. Therefore, early detection and management of hematological and coagulation abnormalities in renal disease patients are critical.