bluuebunny/crossref_metadata_2025_split huggingface dataset:
HPLC-UV Analysis Coupled with Chemometry to Identify Phenolic Biomarkers from Medicinal Plants, used as Ingredients in Two Food Supplement Formulas
Authors: Pop Raluca Maria , Csernatoni Florina , Ranga Floricuta , Fetea Florinela , Socaciu Carmen 
Published: December 2016

. High performance liquid chromatography (HPLC) with UV detection is nowadays the reference method to identify and quantify the biomarkers of quality and authenticity of plants and food supplements. Seven medicinal plants were collected from wild flora: Taraxacum officinalis (1), Cynara scolimus (2), Silybum marianum (3), Hypericum perforatum (4), Chelidonium majus (5), Lycopodium clavatum (6) and Hippophae rhamnoides (7) leaves and fruits. Two products (A and B) were obtained by mixing individual plant powders. Therefore product A was obtained by mixing dandelion, artichoke and milk thistle, 1:1:1 while product B by mixing St John’s wort, Celandine and Wolf’s claw, 1:1:1. The methanolic extracts of individual plants as well as three different extracts of products A and B (using acidulated water, neutral water and acidulated methanol) were analyzed using HPLC-UV for their phenolics’ fingerprint and composition. The qualitative (untargeted analysis) and quantitative (targeted analysis) results were further compared using Principal Component Analysis (PCA) in order to identify their specific biomarkers. Thus, quantitative evaluation of individual phenolics in case of individual plants and products A and B extracts, showed specific and significant differences of composition. Both products A and B contained elagic acid as major compound. For product A, good biomarkers were trans-cinnamic, chlorogenic, caffeic and p-coumaric acids, as well silymarin and silibine originating from milk thistle. For product B, good biomarkers were quercetin and kaempherol, gallic and protocatecuic acids, this product being rich in flavonoids. In conclusion, HPLC-UV coupled with PCA analysis proved to be a rapid and useful way to identify the main biomarkers of plants’ authentication, as well of final products’ quality and safety.
Bioactive Labels for Fresh Fruits and Vegetables
Authors: Liana Nasui , Vodnar Dan , Carmen Socaciu 
Published: December 2016

Pesticide residues and microbial load on the surface of fresh fruits and vegetables becomes a major concern due to the safety and quality of these products for consumer.In order to minimize these risk factors (pesticide residues and microbial load), were achieved labels for fruits and vegetables that are consumed with shell which disintegrates under the influence of water jet and thus reduce the amount of these contaminants. Were elaborated labels based on polymer (chitosan) at a concentration of 2%, which incorporate bioactive compounds from green tea with potential decontaminant of the peel of this products. Green tea extract was obtained by infusing 1 g of dried green tea in 100 ml water at 80° C for 10 minutes. The extract was filtered and then mixed with 2 g chitosan acidified with 0.7% glacial acetic acid and dilute to the mark with distilled water. Were identified bioactive compounds from green tea, using UV-VIS and HPLC. Then were elaborated the labels. These tags were used on pepper, tomato, apple and nectarine. Were quantified the microbial load and the pesticide residues on their surface unwashed, washed only with water and were monitored the influence of labels on these factors. Identified pesticides were mefenoxan and thiamethoxam, which were quantified by HPLC. In what it concerns the influence, were founded the absence of germs at pepper and a significant decrease at the other. In terms of the potential of reducing pesticide, the experimental results have indicated that the label can prove its effectiveness.
Comparative Fingerprint of Aromatic Herbs and Yeast Alcoholic Extracts used as Ingredients for Promen, a Prostate Preventive Nutraceutical
Authors: Csernatoni Florina , Socaciu Carmen , Pop Raluca Maria , Ranga Floricuta , Bunghez Florina , Romanciu Florina 
Published: December 2016

The aim of this study was to characterize and identify different bioactive compounds in plant sources and yeast powders to obtain an original nutraceutical (Promen) which has beneficial effects in prostate disease prevention. Seven plant and fruit sources, namely nettle (Urtica dioica), green tea (Camellia sinensis), fluff with small flowers (Epilobium parviplorum), tomato (Solanum licopersicum), sea buckthorn (Hippophae rhamnoides), pumpkin (Cucurbita maxima), sunflower (Helianthus annus) and lyophilized beer yeast (Saccharomyces cerevisiae) were investigated. Methanolic extracts were prepared using 15% plant concentration and the purified fractions were analyzed using high throughput techniques like UV-VIS spectroscopy, high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD) and mass spectrometry LC-QTOF -MS. The majority of the investigated plants were rich in phenolic derivatives, polyphenols (flavonoid glucosides), while yeast was rich in aminoacids, peptides and vitamins B. The major compounds identified were: Juglone, Resveratrol, Quercetin, Epigallocatechin, Gallocatechin, Biochanin A, Isorhamnetin 3-O-glucoside 7-O-rhamnoside, Quercetin 3-O-galactoside 7-O-rhamnoside, Kaempferol 3,7-O-diglucoside and p-Coumaroylquinic acid. The specific biomarkers were identified for both plant extracts used as ingredients to obtain an nutraceutical Promen. Combined UV-Vis spectroscopy, HPLC-PDA chromatography and LC-MS spectrometry are recommended as accurate, sensible and reliable tools to investigate the plants and nutraceutical fingerprints and to predict the relation between ingredients composition and their health effects.
Effect of Chlorocholine Chloride (CCC) on the Plants’ Height and Inulin Content in Jerusalem Artichoke (Helianthus tuberosus L.)
Authors: Wawrzyniak Mikołaj , Szwengiel Artur , Stelmach-Mardas Marta , Hołubowicz Roman 
Published: December 2016

Jerusalem artichoke (Helianthus tuberosus L.) is herbaceous perennial plant rich in inulin and useful source of biomass. Due to its low agricultural requirements and high adaptability, it can provide very high biomass yields even on low quality sites. The plant is used in food industry, bio-fuel production, forage, pharmacy and nutrition. Its tubers accumulate approx. 10-20% of inulin in fresh weight. Currently, the use of the Helianthius tuberosus L. as a potential dietary strategy in patients affected by type 2 Diabetes is challenge. Moreover, deep understanding of the relationship between diet and composition of gut microbiota can bring the new insight in the treatment of inflammatory dependent diseases. The aim of this study was to examine an effect of plant growth retardant Chlorocholine Chloride (CCC) on the plants’ height of H. tuberosus and inulin content in the tubers. We examined in the field a procedure for its shoots reduction. Material for the experiment were bought in a Polish commercial company and 528 tubers were planted in field in the middle of April 2014. Then, half of them were sprayed with 0.75% retardant of CCC . Furthermore, every week for 12 following weeks, the plants’ heights were measured. After the vegetation was over, 6 tubers for each treatment were dug out and chemically analyzed for inulin content using High Pressure Size Exclusion Chromatography. After first week of CCC use, 16% decrease of the heights plants was observed. Height of plants sprayed with CCC were significantly different comparing to Control. Weekly growth was significantly slower in plants sprayed with CCC on first three weeks after applying retardant. Differences in plants height sustain to the end of measurements. Used retardant and its concentration did not affect the inulin content of the tubers.
Experimentation of grapevine cultivation in organic system, on five different Romanian vineyards
Authors: Ranca Aurora Maria , Artem Victoria , Dina Ionica , Parcalabu Liliana , Iliescu Maria , Tabaranu Gabriel , Nechita Ancuta 
Published: December 2016

In five Romanian vineyards, in 2013-2014 period was experimented organic system growing on grape varieties for wine representative for each area, here are applied organic growing technology in parallel with the conventional (control). White varieties studied are: Chardonnay (Murfatlar and Valea Calugareasca), Sauvignon blanc and Muscat Ottonel(Tarnave), Feteasca regala (Tarnave, Bujoru and Copou-Iasi). Red varieties are Cabernet Sauvignon (Murfatlar and Valea Calugareasca) and Merlot (Bujoru).Concerning evolution of main climatic factors for the years 2013-2014 it is show that the annual average air temperature increase compared with the average of the years 1991-2010; is observed an increasing in mean annual temperature, mean temperature during the growing season and the value of sum of sunshine hours. The water regime was kept constant, registering values close to the annual average, both during the growing season and at entire year. The weeds spectrum is represented both by dicotyledonous as knotweed (Polygonum aviculare), Veronica (Stellaria media), news (Amaranthus retroflexus), bindweed (Convolvurus arvensis) and monocotyledonous as grass thick (Cynodon dactylon).Concerning the phytosanitary status, at Murfatlar were registered problems with oidium attack, with all main disease at Dealu Mare and with downy mildew at Bujoru. In all areas the pest has been found are: grape mouth (Lobesia botrana) and spiders (Tetranichus sp.). Schemes of treatments focused generally on substances bassed on copper and sulfur to combat diseases and for pests have been used pheromonal traps or other certified organic products.The grapes harvest was lower in organic plots with till 25%; their quality being close at both growing variants.
Research About Improvement the Aromatique Grapevine by Selection Clonale in the Vineyard Dealu Bujorului
Authors: Donici Alina , Bora Florin Dumitru , Eneche Vasilica , Tabaranu Gabriel 
Published: December 2016

The research in this paper aims the quality producţion and uvologic values of two elites clonal ‘Muscat Ottonel’ 35-21, ‘Şarbă 25-45’ and two varieties ‘Muscat Ottonel’, ‘Şarbă’ for aromatic white wines cultivated in SCDVV Bujoru. The highest weight of a grape was recorded in the ‘Şarbă 25-45’ (183 g) and the lowest was recorded in ‘Muscat Ottonel’ (93 g). The lowest index structure was recorded in ‘Muscat Ottonel’ (27.0) and the higher was registered in ‘Muscat Ottonel 35-21’.
Preliminary Results on Behavior of Transgenic and Conventional Plum to Natural PPV Infection in a New Field Trial
Authors: Zagrai Ioan , Zagrai Luminita , Festila Angela 
Published: December 2016

To assess the durability of resistance to PPV of HoneySweet transgenic plum harbouring plum pox virus (PPV) capsid gene, a new field trial was recently established in Romania at Fruit Research & Development Station Bistrita. Stanley and Reine Claude d’Althan cvs. are used as control. PPV inoculum source is secured within the experimental plot. PPV monitoring was made by visual observation of symptoms development on leaves and by serological (DAS-ELISA) and molecular (IC/-RT-PCR) testings. Three years after planting PPV was observed and detected only on Stanley. 'HoneySweet' and Reine Claude d’Althan cultivars remained PPV free.
Effect of Different Types of Fertilization on Vigna unguiculata subsp. sesquipedalis Crop
Authors: Angeli Cristina , Cheimona Nicolina , Kakabouki Ioanna , Kontopoulou Charis Konstantina , Tabaxi Ioanna , Papandreou Anastasia , Pachi Vasiliki , Drossinou Ioulia , Papastylianou Panayiota , Bilalis Dimitrios J 
Published: December 2016

A field experiment was conducted at Pylos, Greece to compare the effect of different types of fertilization on yardlong bean (Vigna unguiculata subsp. sesquipedalis) crop. The conventional treatment plots were fertilized with an inorganic fertilizer, whereas the organic treatments plots received organic compost. Data analysis confirmed no significant correlation between plant height and type of fertilization, but there was significant correlation between length of pods and type of fertilization. In particular, morphological characteristics of yardlong bean were enhanced by inorganic fertilization in comparison with the organic fertilization. Organic farming increased significantly the number on root nodules in comparison with conventional farming.
THE IMPACT OF THE STORAGE PLATFORMS FOR HOUSEHOLD WASTE BINS ON THE ENVIRONMENT DEPENDING ON THEIR EMPLACEMENT
Authors: Gașpar Cristina Mirabela , Tibru Ioan 
Published: December 2016

The large amount of stored waste is still a big concern for Romania and according to the legislative provisions, until 16th of July 2016, it has to be reduced at 35% from the total amount of waste stored in 1995 (2). Attempting this goal depends on the extent to which the population perceives the need of selective collection, recycling and residue minimization and on the way in which the local authorities facilitates the proper conduct of this process and ensures compliance with the hygiene and public health standards in relation to the location of household waste pre-collection containers.The aim of this paper was to verify if the previsions contained in the Order no. 119/2014, for the aproval of Hygiene and public health standards regarding the population’s living environment, are complied with.In this regard, there were conceived questionnaires concerning the emplacement of storage platforms for containers used for selective collection of household waste, containing the requirements stipulated in Chapter 1 – Hygiene standards regarding the housing areas, article 4, point a) (1).It has been found that in the living areas taken into study, the requirements stipulated in Order no. 119/2014, for the aproval of Hygiene and public health standards regarding the population’s living environment, in Chapter 1 – Hygiene standards regarding the housing areas, article 4, point a), are not fully respected.
The metabolic profile in laying hens of the Rosso race after 180 days of feed supplement with organic selenium
Authors: Raduta Adrian , Curca Dumitru 
Published: December 2016

Selenium is a trace mineral with antioxidant proprieties, which, by mediating the glutathione, indirectly protects the hemoglobin against the risk of oxidation by peroxides through three antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalaseThe experiment was done on 20 laying chicks from the Rosso race, 6 weeks old at the start. The chicks were divided in 2 batches, and one of the batches received feed enriched with organic selenium (Sel-Plex). After 180 days, biological samples were colected by cubital vein puncture and hematological and biochemical determinations were made. The results were tabled, graphically represented and biostatistically interpreted. In the experimental batch significant growths were observed in the erythrocyte constants: erithremie, hemoglobinemy, hematocrite and MCH. Of the biochemical markers significant growths were observed in: ascorbinemic acid, alkalin phospahasis and selenium. The following parameters dropped significantly : proteinemia, amylase and MCV. Changes were also observed in cholesterol, calcium, magnesium and other parameters, but without statisticaly value. In the present paper we have shown the beneficial role of organic selenium on some haematological and biochemical markers, resulting in a growth of erythropoiesis, and at the same time a intensification of the metabolic processes in the experimental group.
Effects of Pre and Probiotic on Growth Performance and Haematological Parameters in Pigs
Authors: Szakacs Andrei Radu , Matei Sorana , Stefanut Laura , Moni Zoltan , Macri Adrian 
Published: December 2016

The present study investigates the effects of dietary supplementation with feed supplements (prebiotics and probiotics) on pig performance, the feed conversion ratio and some haematological parameters.The research was conducted on hybrid pigs [(Yorkshire × Landrace) × Duroc] in a farm from Sălaj County, Romania. Pigs of 11 weeks (n = 200) were divided into 4 equal groups: a group fed with the probiotic, a prebiotic-fed group, a control group and a synbiotic (pre- and probiotic) treated group. The control group was fed with a feed without the specified additives. Probiotic treated group was fed the same feedstuff but containing the additive Beta Plus® Ultra (Biochem)( 5.12x1012 CFU / kg - Bacillus subtilis DSM 5750, 5.12x1012UFC / kg - Bacillus licheniformis (DSM 5749 ) and 921 g of betaine), in an amount of 1 kg / tonne of feed. Prebiotic group had added to the feed an extract derived from the cell wall of Saccharomyces cerevisiae (TechnoMos® - Biochem) in a dose of 250 g / ton of feed. The weight of 25 pigs in each compartment was recorded 9 times (over 85 days). Animal health was evaluated via clinical, haematological, and parasitological examinations. 5 rectal swabs were collected on day 11 and day 77 and faeces samples were taken in day 77 from each investigated group. Growth rate, feed consumption and some haematological parameters were measured.The body weight, average total weight gain and feed conversion rate increase by the dietary inclusion of the both pre and probiotic (p˃0.05). In the compartment treated with prebiotic the weight of the pigs has increased by 1030 g/day compared with 982 g/day value recorded in the control group. The average feed consumption was 3.21 kg/day in the control group while the group treated with prebiotic was only 2.99 kg/day. Feed conversion rate for all treated groups were lower than the one of control. Haematological parameters varied in physiological limits of species.Conclusion: These products show promising effects as an alternative for antibiotics in order to eliminate the use of these drugs as growth-promoting additives.
REVERSE PDA – LESS COMMON TYPE OF PATENT DUCTUS ARTERIOSUS -CASE REPORT
Authors: Scurtu Iuliu , Pestean Cosmin , Lacatus Radu , Lascu Meda , Mircean Mircea , Codea Razvan , Popovici Cristian , Purdoiu Robert , Giurgiu Gabril 
Published: December 2016

Introduction: PDA represents one of the most frequently diagnosed type of congenital heart disease. Ductus arteriosus is a normal structure in foetal life, which permits shunting of oxygenated blood from the pulmonary artery into the aorta. Failure of sealing after birth is an abnormal condition and is called patent ductus arteriosus. In normal PDA, due to fact that systemic pressure is fivefold higher than pulmonary circulation, blood is shunted from the aorta into the pulmonary artery. In reverse PDA, pulmonary artery pressure does not drop after birth, and blood will be shunted form right to left. Aims: We want to evaluate clinical, haematological, ECG and echocardiographic changes in case of reverse PDA. Materials and Methods: Two-year old female Bichon Frise was referred to our clinic with signs of effort intolerance and dyspnoea for more than a year. ECG was performed in the right lateral recumbency using a digital device and echocardiography was done with Esaote MyLab40 Vet with a phased array transducer matched with the size of the dog (7.5 MHz). Results: We identified a dog with a good body score, quite alert and without any sign of illness. Haematological investigation underlined polycythaemia and very high PCV. The ECG revealed a normal sinus rhythm with a deep S wave, changes consistent with right ventricle enlargement. Right atrial dilation and right ventricle hypertrophy were found on cardiac ultrasonography. The right ventricle free wall was hypertrophied and interventricular septum was flattened, changes consistent with increased pressure on the right side of the heart. The left heart was small. Positive diagnosis was done, performing “bubble study” and identification of contrast bubble within the abdominal aorta. Conclusion: Reverse PDA is a rarely diagnosed congenital heart disease. Polycythaemia in young dogs could raise the suspicion of reverse PDA. For positive diagnosis, echocardiography and bubble study are required. ECG is not a sensitive tool for diagnosis.
Microbiological Hazards Assessment of Psychrotrophic Microflora in Bovine Carcasses Slaughtered in North West Romania
Authors: Vida Silviu , Dan Sorin Daniel , Tabaran Alexandra , Reget Oana Lucia , Fat Alina Ioana , Mihaiu Marian 
Published: December 2016

Gram negative bacteria have the greatest capacity to spoil the meat if kept under aerobic conditions, therefor the members of the genera Pseudomonas, Acinetobater, Psychrobacter and Moraxella, will form the dominant microflora. The purpose of this study was to assess the contamination level with psychrotropic microflora of bovine carcasses in order to prove how the results could be used to improve the slaughter process. The research was carried out between January-December 2015, in two slaughterhouses from North West Romania. The research material was represented by 144 meat samples (slaughterhouse A, n=72, slaughterhouse B, n=72). Weekly, 3 samples were collected from refrigerated carcasses, and examined for total psychrotrophic counts (TPC), Enterobacteriaceae, Pseudomonas, Aeromonas and Yersinia. From statistical processing of recorded data was established that mean log TPC from the surface of refrigerated carcasses has presented different values, ranged between 3.70±0.20 log CFU/cm2 and 6.90±0.43 log CFU/cm2. Initial surface microflora of bovine carcasses was represented by germs from the following genera: Staphylococcus, Micrococcus, Aeromonas, Acinetobacter, Pseudomonas, Yersinia, Serratia, Hafnia, Proteus and Escherichia. Microbiological hazard assessment reveals the key role of psychotropic microorganisms in the spoilage of meat, if the monitoring system of the slaughtering process is not functioning properly.
UN ESTUDIO GENERAL DE LAS ARTES Y LA POLÍTICA DE NEPAL DURANTE EL SIGLO XX: PROCESOS DE TRANSICIÓN Y DIÁLOGO MULTICULTURAL.
Authors: De la Rubia Gomez-Moran Andrea 
Published: December 2016

<p>Este trabajo consiste en un estudio generalizado del desarrollo del arte moderno nepalí en torno a los años 50, cuando el país comenzó un proceso de modernización como consecuencia de su reapertura al mundo exterior y consiguiente relación con las culturas extranjeras. Durante la transición, tanto el cambio político producido por las nuevas ideas democráticas como los nuevos medios de comunicación y consumo contribuyeron a la creación de un nuevo Nepal, dividido entre lo moderno y lo tradicional. El objetivo de este estudio es el de subrayar que el arte moderno de Nepal se desarrolló a partir de su propia localidad, pero también teniendo en cuenta la influencia que sobre el arte nepalí han ejercido los procesos capitalistas y globalización cultural.</p>
La Section d’Or y Juan Gris: Un camino hacia un cubismo espiritual
Authors: Sarriugarte Gomez Iñigo 
Published: December 2016

<p>El artista de origen madrileño Juan Gris tuvo la oportunidad de ser testigo directo de la gestación del cubismo a raíz de su estancia en 1906 en la capital francesa. A partir de aquí, comienza a relacionarse con distintos pintores y críticos vanguardistas, que le ofertarán nuevas posibilidades aperturistas para su posterior involucración cubista. El año 1912 marca su definitiva integración bajo la estela pictórica del cubismo sintético, destacando igualmente su relación con el grupo de <em>Puteaux</em>. Los encuentros con esta agrupación cubista le derivarán hacia postulados caracterizados por la aplicación de enrejados, que eran formulados bajo premisas matemáticas, así como un interés manifiesto por la sección aurea, pero sin ser olvidado por parte del artista la evocación de un discurso espiritual adecuado a las necesidades de su tiempo. </p>