bluuebunny/crossref_metadata_2025_split huggingface dataset:
MAP7 Regulates Axon Collateral Branch Development in Dorsal Root Ganglion Neurons
Authors: Tymanskyj Stephen R. , Yang Benjamin , Falnikar Aditi , Lepore Angelo C. , Ma Le 
Published: January 2017

Collateral branches from axons are key components of functional neural circuits that allow neurons to connect with multiple synaptic targets. Like axon growth and guidance, formation of collateral branches depends on the regulation of microtubules, but how such regulation is coordinated to ensure proper circuit development is not known. Based on microarray analysis, we have identified a role for microtubule-associated protein 7 (MAP7) during collateral branch development of dorsal root ganglion (DRG) sensory neurons. We show that MAP7 is expressed at the onset of collateral branch formation. Perturbation of its expression by overexpression or shRNA knockdown alters axon branching in cultured DRG neurons. Localization and time-lapse imaging analysis reveals that MAP7 is enriched at branch points and colocalizes with stable microtubules, but enters the new branch with a delay, suggesting a role in branch maturation. We have also investigated a spontaneous mutant mouse that expresses a truncated MAP7 and found a gain-of-function phenotype bothin vitroandin vivo. Further domain analysis suggests that the amino half of MAP7 is responsible for branch formation, suggesting a mechanism that is independent of its known interaction with kinesin. Moreover, this mouse exhibits increased pain sensitivity, a phenotype that is consistent with increased collateral branch formation. Therefore, our study not only uncovers the first neuronal function of MAP7, but also demonstrates the importance of proper microtubule regulation in neural circuit development. Furthermore, our data provide new insights into microtubule regulation during axonal morphogenesis and may shed light on MAP7 function in neurological disorders.SIGNIFICANCE STATEMENTNeurons communicate with multiple targets by forming axonal branches. In search of intrinsic factors that control collateral branch development, we identified a role for microtubule-associated protein 7 (MAP7) in dorsal root ganglion sensory neurons. We show that MAP7 expression is developmentally regulated and perturbation of this expression alters branch formation. Cell biological analysis indicates that MAP7 promotes branch maturation. Analysis of a spontaneous mouse mutant suggests a molecular mechanism for branch regulation and the potential influence of collateral branches on pain sensitivity. Our studies thus establish the first neuronal function of MAP7 and demonstrate its role in branch morphogenesis and neural circuit function. These findings may help in our understanding of the contribution of MAP7 to neurological disorders and nerve regeneration.
TFIID or not TFIID, a continuing transcriptional SAGA
Authors: Kubik Slawomir , Bruzzone Maria Jessica , Shore David 
Published: January 2017

Eukaryotic protein‐coding genes are typically classified into two groups: those with expression regulated by specific signals versus the relatively constant “housekeeping” genes. Although these differences are associated with alternative modes of RNA polymerase II (RNAP II) pre‐initiation complex (PIC) assembly, a role for gene‐specific activators in controlling “regulatability” has been difficult to rule out. To address this question, de Jonge et al () studied a group of genes controlled by a common activator but dependent on either TFIID or SAGA and found that the magnitude of regulation strongly correlates with the mechanism of PIC assembly.
Libros de la Universidad de Santo Tomás preservados en la Biblioteca Nacional
Authors: González-Arrieta Ruth , Moya López Xinia 
Published: January 2017

La Universidad de Santo Tomás fue la primera institución de Educación Superior costarricense, creada en 1843 por el Dr. José María Castro Madriz, por lo que se constituyó como universidad pionera del país. Inició labores universitarias en 1844, año en que ingresó para la Biblioteca, el primer embarque de libros provenientes de Europa. Con el transcurso del tiempo, la adquisición de textos afines a las materias de estudio como leyes, filosofía, literatura, gramática, teología e historia, entre otras, se acrecentó y, por ende, la colección bibliográfica aumentó. A pesar de la floreciente universidad, decisiones políticas incidieron en el cierre de la institución en 1888. Con la clausura de la universidad, el educador Miguel Obregón Lizano impulsó que las colecciones de libros de la Biblioteca Universitaria se trasladaran a otra unidad de información, dando origen a la Biblioteca Nacional de Costa Rica, que ostenta su nombre y desde entonces resguarda los libros de la Universidad de Santo Tomás
Association of health symptoms with low-level exposure to organophosphates, DNA damage, AChE activity, and occupational knowledge and practice among rice, corn, and double-crop farmers
Authors: Hongsibsong Surat , Sittitoon Nalin , Sapbamrer Ratana 
Published: January 2017

Abstract

Objectives
This study aims to determine (1) total dialkylphosphate (ΣDAP) levels, occupational knowledge and practice, DNA damage, AChE activity, and health symptoms in rice, corn, and double-crop farmers; (2) the association of health symptoms with ΣDAP levels, occupational knowledge and practice, DNA damage, and AChE activity in farmers; and (3) the prevalence of health symptoms between farmers and nonfarmers.

Methods
A cross-sectional study was conducted by interviewing as well as analyzing urine and blood samples during July to August 2014.

Results
There were no differences in ΣDAP levels, AChE activity, and occupational knowledge and practice scores among all farmer groups. In terms of health symptoms related to ΣDAP, AChE activity, DNA damage, and occupational knowledge and practice, pesticide-related symptoms were determined, including breathlessness, chest pain, dry throat, numbness, muscle weakness, cramp, headache, dizziness, eye irritation, white/red rash, and white/red pimple, which were classified as respiratory, muscle, nervous, and epithelial symptoms. A remarkable finding was that farmers had a significantly higher prevalence of muscle weakness (odds ratio (OR)=3.79) and numbness (OR=3.45) as compared with non-farmers.

Conclusion
Our findings, therefore, suggest that a long-term lowlevel exposure to organophosphates (OPs) may be associated with an increasing prevalence of muscle symptoms. However, a further cohort study incorporating sensitive health outcomes and measurement of multiple pesticides monitoring on a larger scale is warranted.
Burnout among public servants after the Great East Japan Earthquake: decomposing the construct aftermath of disaster
Authors: Suzuki Yuriko , Fukasawa Maiko , Obara Akiko , Kim Yoshiharu 
Published: January 2017

Abstract

Objectives
To examine whether disasterrelated variables, in addition to known work-related risk factors, influence burnout and its subscales (exhaustion, cynicism, and lack of professional efficacy) among public servants who experienced a major disaster.

Methods
Cross-sectional studies were conducted among public servants of Miyagi prefecture at 2 and 16 months after the Great East Japan Earthquake (n=3,533, response rate 66.8%); burnout was assessed at 16 months using the Japanese version of the Maslach Burnout Inventory-General Survey. We examined the relationships between burnout and its subscales with disaster-related variables at 2 months after the disaster, while controlling for age, gender, and work-related variables at 16 months after the disaster.

Results
After controlling for age, gender, and work-related variables, a significant risk factor of burnout was having severe house damage. For the each subscale of burnout, living someplace other than their own house increased the risk of both exhaustion and cynicism, while handling residents’ complaints did so only for exhaustion. Notably, workers from health and welfare departments showed an increased risk of burnout, exhaustion, and cynicism, but not lack of professional efficacy.

Conclusions
The findings suggest that special attention is needed for workers with severe house damage to prevent burnout, as well as those who lived someplace other than their own house to prevent exhaustion and cynicism after a major disaster. Interventions directed at workers of the health and welfare department should focus more on limiting exhaustion and cynicism, rather than promoting professional efficacy.
Oficina de leitura e escrita inspirada em literatura, cinema e fantástico
Authors: Camilo Alessandra Da Rosa Trindade , Becker Simone , Fontanella Valdirene Alves 
Published: January 2017

Na época atual, a linguagem não verbal vem ganhando espaço e sendo explorada por seu caráter pedagógico em sala de aula. Neste artigo, é possível observar que, enquanto a linguagem do cinema utiliza recursos de som e imagem, a linguagem da literatura induz o leitor a imaginar o que se passa além das palavras a partir de sua experiência pessoal. Nesse sentido, foi realizada uma oficina de processos de leitura e escrita, em um curso de graduação de licenciatura em letras, baseada em trechos da versão escrita (LOWRY, 2014) e da versão fílmica (NOYCE, 2014) da obra O Doador de Memórias (The Giver, 1983). A oficina teve como objetivos promover a interação e a reflexão sobre a leitura de sentidos de palavras em diferentes contextos e suportes, bem como o estímulo da produção escrita espontânea e criativa, a apresentação de múltiplas visões dos alunos e a colocação deles como sujeitos no processo. Como fator instigante, a obra ficcional utilizada presentava características do fantástico. O artigo encontra embasamento para interação e contextualização em Bakhtin, Garcez, Scnheuwly, Rodrigues; Cerutti-Rizatti e Kleiman, o conceito de fantástico em Todorov e a relação entre obras fílmicas e literárias em Bourdieu, Fabris e Duarte.
Intervenção precoce nas dificuldades de aprendizagem da leitura com recurso ao software Graphogame1
Authors: Sucena Ana , Silva Ana Filipa , Viana Fernanda Leopoldina 
Published: January 2017

Neste estudo avaliou-se o impacto de um software de apoio às dificuldades de aprendizagem da leitura – o Graphogame – ao nível das relações letra-som, consciência fonémica, leitura de palavras e leitura de pseudopalavras. Os participantes eram 57 crianças, a frequentar o 1º ano de escolaridade, falantes nativos do português europeu, identificadas como em risco de virem a apresentar dificuldades de aprendizagem da leitura. As crianças foram divididas em três grupos: (a) intervenção com recurso a software – treino com software Graphogame, (b) intervenção mista – treino com recurso ao software Graphogame e a sessões de competências pré-leitoras e leitoras orientadas por técnico de educação e (c) ausência de intervenção para lá daquela prevista no sistema regular de ensino. Os resultados revelam efeitos positivos de ambos os tipos de intervenção em relação ao grupo controlo. Revelam ainda um efeito mais pronunciado para o grupo de intervenção mista do que para o grupo de intervenção com recurso exclusivo ao software.
Intertextualidade e compreensão leitora: ensino da leitura com apoio da tecnologia
Authors: Baretta Danielle , Chaves Jésura Lopes 
Published: January 2017

O presente trabalho tem por objetivo demonstrar como a tecnologia pode apoiar o ensino, apresentando um objeto de aprendizagem voltado para o ensino de leitura. Nesse sentido, o objeto de aprendizagem, intitulado Fábulas Revisitadas, parte da concepção de que a compreensão leitora é fator central e essencial nas aulas de Língua Portuguesa e de que a escola é a responsável por promover a ampliação dos conhecimentos prévios do educando, tornando-o capaz de interpretar diferentes textos que circulam socialmente. Sendo assim, o objeto de aprendizagem em questão pretende desenvolver essa habilidade a partir da intertextualidade. Para tanto, apresenta blocos de atividades organizados em torno de cinco fábulas, nos quais o texto original é confrontado com outros textos que com ele dialogam. Espera-se, com isso, que os alunos reconheçam o papel dos conhecimentos prévios para uma efetiva compreensão leitora, bem como as pistas que permitem identificar o diálogo entre textos e os sentidos dele decorrentes.
Gobierno abierto en bibliotecas públicas: planeación estratégica y el valor público
Authors: Voutssás Lara Jennifer Alejandra 
Published: January 2017

La información no es la única que ha cambiado sus modelos a través de las herramientas digitales, sino que la misma sociedad se transforma constantemente en nuevos modelos y relaciones sociales. Esto ha obligado a las instituciones públicas a replantear también su labor en cuanto a la oferta de servicios a los ciudadanos, por lo que ha planteado un modelo basado en la administración de empresas privadas para una mejor administración en el ámbito público y optimización de los servicios que se ofrecen. Esta investigación ofrece un panorama conceptual del gobierno abierto, los conceptos de planeación estratégica y valor público para ejemplificar cómo fueron implementados en las bibliotecas públicas que atienden ahora a ciudadanos en ejercicio principalmente del derecho a la información y, a su vez, permitiendo el ejercicio de otros derechos fundamentales. Para ello, fue utilizada como metodología a través del análisis documental de fuentes directas sobre gobierno abierto a nivel mundial y de instituciones públicas internacionales que vigilan este derecho; así como casos en diversas bibliotecas públicas en Estados Unidos donde se llevaron estrategias de gobierno abierto en bibliotecas públicas. Finalmente, se consideraron las fuentes indirectas; por ejemplo, información no referente a estos conceptos, pero que ejemplifican el valor público y la planeación estratégica de las bibliotecas públicas en México y que, por medio del encadenamiento deductivo, complementan la comprensión del gobierno abierto en las bibliotecas como instituciones públicas y proponer nuevas estrategias de valor público en estos espacios.
VARIACIONES EN LA FLEXIBILIDAD DURANTE EL CICLO MENSTRUAL EN DEPORTISTAS UNIVERSITARIAS
Authors: Moiso Marín Fiorella , Solera Herrera Andrea 
Published: January 2017

Con el objetivo de indagar si existen variaciones en la flexibilidad en 2 fases del ciclo menstrual (menstrual y ovulatoria) de atletas universitarias, se aplicaron pruebas de flexibilidad funcionales y multi-articulares a 28 mujeres con un promedio de edad de 20.9 ± 3.0 años, nivel de estrés moderado, pocas horas de sueño relacionadas con niveles altos de estrés, y un hábito de consumo de alcohol de al menos una vez al mes en la mayoría de ellas. A nivel general, la t de Student no encontró variaciones significativas en la flexibilidad a lo largo del ciclo menstrual. El nivel de flexibilidad en la prueba de giro de hombros en anteversión fue de 50.73 ± 17.97º y de 51.97 ± 16.76º (t = -1.031; p = 0.312), en retroversión fue de 58.14 ± 17.28º y 60.53 ± 17.12º (t = -1.754; p = 0.091) y en la prueba de Sit and Reach fue de 110.63 ± 7.56 cm y 110.48 ± 8.31 cm (t = 0.252; p = 0.803) en la fase ovulatoria y menstrual respectivamente. No obstante, la t de Student estratificada, encontró mayor flexibilidad en la fase ovulatoria en mujeres que consumían regularmente alcohol (p= 0.020) y tenían un nivel de estrés más alto (p= 0.040) para la prueba de giro de hombros en anteversión, y en mujeres que no dormían bien (p= 0.030), dormían menos de 7 horas diarias (p= 0.040) o tenían una fase lútea más corta- igual o menor a 9 días- (p= 0,006) para la prueba de giro de hombros en retroversión. En conclusión, no hubo variaciones significativas en la flexibilidad a lo largo del ciclo menstrual en la población general. Sin embargo, ciertas condiciones socio-demográficas y clínicas parecieran predisponer a variaciones durante el mismo.
Diet quality and associated factors among elementary school students of the Adventist school system in the Southwest region of São Paulo, Brazil
Authors: Fuentes Hazel Yvette , Silva Pereira Taísa Sabrina , Portes Leslie Andrews , Fortes de Oliveira Kevin , Teixeira Martins Márcia Cristina 
Published: January 2017

Objetivo: evaluar la asociación entre la dieta y factores socioeconómicos y demográficos entre los estudiantes de la escuela primaria. Métodos: Un estudio transseccional que involucra una muestra aleatoria de 256 niños de ambos sexos, de seis a once años, del primero a quinto grado de siete escuelas adventistas ubicados en la región suroeste de Sao Paulo. Estas escuelas inscriben estudiantes sin importar su religión. Datos demográficos y socioeconómicos se obtuvieron a partir de un cuestionario autoadministrado, llevado a casa y completado por el padre o responsable del niño. La calidad de la dieta se evaluó mediante el índice de ALES. Se calcularon las odds ratio brutas y ajustadas y se utilizó la regresión logística para el análisis de las variables. Resultados: De la muestra, 33,2% tuvo una puntuación pobre en el índice ALES y el 66,8% obtuvo una buena puntuación. No hubo diferencias significativas entre la calidad de la dieta y las siguientes variables: edad, nivel socioeconómico, educación maternal, jefe de la familia, trabajo materno fuera del hogar, lactancia materna exclusiva hasta los 6 meses, y el origen de las meriendas escolares. Las variables que se mantuvieron asociadas a una dieta de buena calidad fueron: ser del sexo femenino (OR = 0.53; 95% IC, 0.30-0.92) y ser Adventista (OR = 2.52; 95% IC, 1.10-5.78). Conclusión: Los factores asociados con la buena calidad de la dieta fueron el sexo y la religión.
Mortalidad por Diabetes tipo 2 en las fronteras de México
Authors: Manzanares Rivera José Luis 
Published: January 2017

Objetivo: Analizar la distribución geográfica y evolución temporal de las tasas de mortalidad por diabetes tipo II en el periodo 1998-2013 para las regiones frontera sur y norte de México. Métodos: el trabajo se basa en el análisis exploratorio e inferencial de datos a partir de los registros de mortalidad reportados por el sistema nacional de información en salud SINAIS. El análisis retoma el paradigma teórico sobre determinantes sociales de salud por lo que se consideran microdatos sobre los patrones de consumo a nivel hogar en el contexto de la frontera México-Estados Unidos y México-Guatemala. Resultados: Se encuentra una trayectoria de convergencia durante los últimos 15 años en las tasas de mortalidad por diabetes tipo II entre las dos regiones fronterizas estudiadas. Conclusiones: La evidencia presentada indica que los estados de la frontera sur han experimentado en la última década una transición epidemiológica hacia causas de mortalidad como la diabetes tipo II, una trayectoria que muestra un patrón de convergencia con la población en la frontera norte.
Comparison of mast Burkholderia cepacia, ashdown + gentamicin, and Burkholderia pseudomallei selective agar for the selective growth of Burkholderia spp.
Authors: Edler Carola , Derschum Henri , Köhler Mirko , Neubauer Heinrich , Frickmann Hagen , Hagen Ralf Matthias 
Published: January 2017

Reliable identification of pathogenic Burkholderia spp. like Burkholderia mallei and Burkholderia pseudomallei in clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with various Burkholderia spp. and nontarget organisms.

Mast Burkholderia cepacia agar, Ashdown + gentamicin agar, and B. pseudomallei selective agar were compared. A panel of 116 reference strains and well-characterized clinical isolates, comprising 30 B. pseudomallei, 20 B. mallei, 18 other Burkholderia spp., and 48 nontarget organisms, was used for this assessment.
While all B. pseudomallei strains grew on all three tested selective agars, the other Burkholderia spp. showed a diverse growth pattern. Nontarget organisms, i.e., nonfermentative rod-shaped bacteria, other species, and yeasts, grew on all selective agars. Colony morphology did not allow unambiguous discrimination.
While the assessed selective media reliably allowed the growth of a wide range of B. pseudomallei strains, growth of other Burkholderia spp. is only partially ensured. Growth of various nontarget organisms has to be considered. Therefore, the assessed media can only be used in combination with other confirmative tests in the diagnostic procedure for the screening for melioidosis or glanders.
Micro-Photoluminescence from V-shape inverted pyramid in HVPE Grown GaN Film
Authors: Lee Chao-Kuei , Chen Y-B , Chang Shu-Chen , Pan Ci-Ling , Wang S. C. 
Published: January 2017

AbstractWe report the room-temperature micro-luminescence images from V-shaped inverted pyramids in undoped GaN films grown on (0001) sapphire substrate by hybrid vapor phase epitaxy. As the excitation laser spot at 325 nm was translated from the surface toward the center of the inverted pyramid along its slope, the center wavelength of the PL peak shows a trend of monotonic red-shift of from 373.9 nm to 379.1 nm. This could be attributed to the 3-dimensional release of stress and associated decrease of build-in piezoelectric field in the V-defects. A distinct and strong emission at 386.7 nm was observed at the apex of the V-defect. This could be originated from the threading dislocation at the onset of the defect.
Growth of Gan on Porous Sic Substrates by Plasma-Assisted Molecular Beam Epitaxy
Authors: Inoki C. K. , Kuan T. S. , Lee C. D. , Sagar Ashutosh , Feenstra R. M. 
Published: January 2017

AbstractWe have explored the growth of GaN on porous SiC substrates by plasma-assisted molecular beam epitaxy. The porous 4H- and 6H-SiC(0001) substrates used in this study contain 10 to 100-nm sized pores and a thin skin layer at the surface. This skin layer was partially removed prior to the growth by H-etching. Transmission electron microscopy (TEM) observations indicate that the epitaxial GaN growth initiates from the surface areas between pores, and the exposed surface pores tend to extend into GaN as open tubes and trap Ga droplets. Plan-view TEM observations indicate that the GaN layers grown on porous substrates contain fewer dislocations than layers grown on non-porous substrates by roughly a factor of two. The GaN layers grown on a porous SiC substrate were also found to be mechanically more relaxed than those grown on non-porous substrates; electron diffraction patterns indicate that the former are free of misfit strain or are even in tension after cooling to room temperature.
Dislocation reduction with quantum dots in GaN grown on sapphire substrates by molecular beam epitaxy
Authors: Smith David J. , Huang Daming , Reshchikov Michael A , Yun Feng , King T. , Morkoç Hadis , Litton Cole W 
Published: January 2017

AbstractWe have investigated a novel approach for improving GaN crystal quality by utilizing a stack of quantum dots (QDs) in GaN grown on sapphire substrates by molecular beam epitaxy. The GaN films were grown on GaN/AlN buffer layers containing multiple QDs and characterized using x-ray diffraction, photoluminescence, atomic force microscopy, and transmission electron microscopy. The density of the dislocations in the films was determined by defect delineation wet chemical etching and atomic force microscopy. It was found that the insertion of a set of multiple GaN QD layers in the buffer layer effectively reduced the density of the dislocations in the epitaxial layers. As compared to a density of ∼1010cm-2in typical GaN films grown on AlN buffer layers, a density of ∼3×107cm-2was demonstrated in GaN films grown with the QD layers. Transmission electron microscopy observations confirmed termination of threading dislocations by the QD layers.