bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nothing to laugh about – the ongoing illegal trade in laughingthrushes (Garrulax species) in the bird markets of Java, Indonesia
Authors: SHEPHERD CHRIS R. , EATON JAMES A. , CHNG SERENE C. L. 
Published: October 2016

SummaryIn ad hoc survey inventories of eight major bird markets in Java in 2014 and 2015, 615 individuals from nine species of the Garrulax genus were found for sale. The most numerous species was Sunda Laughingthrush Garrulax palliatus (215 individuals), followed by Chinese Hwamei G. canorus and Chestnut-capped Laughingthrush G. mitratus. Prices collected in Jakarta revealed that non-native species were the most expensive. Information from these and previous surveys indicate that prices for Sumatran Laughingthrush Garrulax bicolor appeared to have soared since 2007, suggesting increasing rarity of the species. We urge the Indonesian Government to take action against the illegal trade in laughingthrushes under existing laws, especially for the Rufous-fronted Laughingthrush Garrulax rufifrons which is listed on the national protected species list. We also recommend that the Sumatran Laughingthrush Garrulax bicolor be listed as a protected species under Indonesian law. As wild populations of Rufous-fronted Laughingthrush and Sumatran Laughingthrush are threatened by trade, we recommend an urgent review of the conservation status of both species on the IUCN Red List.
Circulating Scholarship: A Note on a Sanskrit Letter from Bengal circa 1535 ce
Authors: WRIGHT SAMUEL 
Published: October 2016

AbstractThis note examines a Sanskrit letter from Bengal dating to approximately 1535 ce. The letter was assumed to be lost. Addressed to a scholar in the discipline of nyāyaśāstra, the letter is the only available example of a personal letter between Sanskrit scholars from Bengal in the premodern period. First located in 1907 and then subsequently assumed lost, the letter has never been the subject of sustained analysis. This has resulted in a number of disagreements among modern scholars about the identities of the individuals named in the letter. This note provides a translation of the letter and presents arguments for identifying the individuals named in the letter. It then concludes by briefly reflecting upon the importance of the letter and its use in scholarly communication.
Impact of explained v. unexplained front-of-package nutrition labels on parent and child food choices: a randomized trial
Authors: Graham Dan J , Lucas-Thompson Rachel G , Mueller Megan P , Jaeb Melanie , Harnack Lisa 
Published: October 2016

AbstractObjectiveThe present study investigated whether parent/child pairs would select more healthful foods when: (i) products were labelled with front-of-package (FOP) nutrition labels relative to packages without labels; (ii) products were labelled with colour-coded Multiple Traffic Light (MTL) FOP labels relative to monochromatic Facts up Front (FuF) FOP labels; and (iii) FOP labels were explained via in-aisle signage v. unexplained.DesignParticipants were randomly assigned to one of five conditions: (i) FuF labels with in-aisle signs explaining the labels; (ii) FuF labels, no signage; (iii) MTL labels with in-aisle signage; (iv) MTL labels, no signage; (v) control group, no labels/signage. Saturated fat, sodium, sugar and energy (calorie) content were compared across conditions.SettingThe study took place in a laboratory grocery aisle.SubjectsParent/child pairs (n 153) completed the study.ResultsResults did not support the hypothesis that MTL labels would lead to more healthful choices than FuF labels. The presence of FOP labels did little to improve the healthfulness of selected foods, with few exceptions (participants with v. without access to FOP labels selected lower-calorie cereals, participants with access to both FOP labels and in-aisle explanatory signage selected products with less saturated fat v. participants without explanatory signage).ConclusionsNeither MTL nor FuF FOP labels led to food choices with significantly lower saturated fat, sodium or sugar. In-aisle signs explaining the FOP labels were somewhat helpful to consumers in making more healthful dietary decisions. New FOP labelling programmes could benefit from campaigns to increase consumer awareness and understanding of the labels.
Heme biomolecule as redox mediator and oxygen shuttle for efficient charging of lithium-oxygen batteries
Authors: Ryu Won-Hee , Gittleson Forrest S. , Thomsen Julianne M. , Li Jinyang , Schwab Mark J. , Brudvig Gary W. , Taylor André D. 
Published: October 2016

AbstractOne of the greatest challenges with lithium-oxygen batteries involves identifying catalysts that facilitate the growth and evolution of cathode species on an oxygen electrode. Heterogeneous solid catalysts cannot adequately address the problematic overpotentials when the surfaces become passivated. However, there exists a class of biomolecules which have been designed by nature to guide complex solution-based oxygen chemistries. Here, we show that the heme molecule, a common porphyrin cofactor in blood, can function as a soluble redox catalyst and oxygen shuttle for efficient oxygen evolution in non-aqueous Li-O2 batteries. The heme’s oxygen binding capability facilitates battery recharge by accepting and releasing dissociated oxygen species while benefiting charge transfer with the cathode. We reveal the chemical change of heme redox molecules where synergy exists with the electrolyte species. This study brings focus to the rational design of solution-based catalysts and suggests a sustainable cross-link between biomolecules and advanced energy storage.
Identification of reference genes for circulating microRNA analysis in colorectal cancer
Authors: Niu Yanqin , Wu Yike , Huang Jinyong , Li Qing , Kang Kang , Qu Junle , Li Furong , Gou Deming 
Published: October 2016

AbstractQuantitative real-time PCR (qPCR) is the most frequently used method for measuring expression levels of microRNAs (miRNAs), which is based on normalization to endogenous references. Although circulating miRNAs have been regarded as potential non-invasive biomarker of disease, no study has been performed so far on reference miRNAs for normalization in colorectal cancer. In this study we tried to identify optimal reference miRNAs for qPCR analysis across colorectal cancer patients and healthy individuals. 485 blood-derived miRNAs were profiled in serum sample pools of both colorectal cancer and healthy control. Seven candidate miRNAs chosen from profiling results as well as three previous reported reference miRNAs were validated using qPCR in 30 colorectal cancer patients and 30 healthy individuals, and thereafter analyzed by statistical algorithms BestKeeper, geNorm and NormFinder. Taken together, hsa-miR-93-5p, hsa-miR-25-3p and hsa-miR-106b-5p were recommended as a set of suitable reference genes. More interestingly, the three miRNAs validated from 485 miRNAs are derived from a single primary transcript, indicting the cluster may be highly conserved in colorectal cancer. However, all three miRNAs differed significantly between healthy individuals and non-small cell lung cancer or breast cancer patients and could not be used as reference genes in the two types of cancer.
Amino acid-based ionic liquid surface modification of magnetic nanoparticles for the magnetic solid-phase extraction of heme proteins
Authors: Wang Binghai , Wang Xiong , Wang Juanqiang , Xue Xue , Xi Xingjun , Chu Qiao , Dong Genlai , Wei Yun 
Published: October 2016

Amino acid-based ionic liquid functionalized magnetic nanoparticles were fabricated as an MSPE adsorbent for hemoglobin with a binding capacity of 1.58 g g−1.
Using Pneumococcal Carriage Data to Monitor Postvaccination Changes in the Incidence of Pneumococcal Otitis Media
Authors: Flasche Stefan , Givon-Lavi Noga , Dagan Ron 
Published: October 2016

AbstractPneumococcal conjugate vaccines (PCVs) have substantially reduced the burden of pneumococcal disease, including the incidence of otitis media (OM). However, in most countries, no surveillance exists to monitor the change in pneumococcal OM incidence after the introduction of PCVs. We explored whether measuring pneumococcal carriage was a useful surrogate for monitoring postvaccination changes in the incidence of pneumococcal OM. The 7-valent PCV was introduced to Israel's national immunization program in July 2009 and gradually replaced by the 13-valent PCV starting in November 2010. Each day since 2009, nasopharyngeal swabs have been obtained from the first 4 Bedouin children and the first 4 Jewish children who were younger than 5 years old and attended a pediatric emergency room in southern Israel. During the same time, OM surveillance in southern Israel included all children younger than 2 years of age who were diagnosed with OM and had undergone a middle-ear fluid culture. The relative change in the prevalence of vaccine-serotype (VT) pneumococcal carriage was predictive of the relative change in incidence of OM due to VT pneumococcus. However, the serotype replacement observed in non-VT carriage is not paralleled in the incidence of OM due to non-VT pneumococcus. This could indicate that there are more complex mechanisms of the immune response involved in preventing initial and consecutive episodes of OM, which has been changed through declining prevalence of the most virulent serotypes as a result of vaccination.
Comparison of Dietary Intakes Between a Large Online Cohort Study (Etude NutriNet-Santé) and a Nationally Representative Cross-Sectional Study (Etude Nationale Nutrition Santé) in France: Addressing the Issue of Generalizability in E-Epidemiology
Authors: Andreeva Valentina A. , Deschamps Valérie , Salanave Benoît , Castetbon Katia , Verdot Charlotte , Kesse-Guyot Emmanuelle , Hercberg Serge 
Published: October 2016

Abstract
Despite some advantages over traditional methods, Web-based studies elicit concerns about generalizability. To address this issue, we compared dietary intakes between an electronic (e-) cohort study and a nationally representative survey. We studied 49,443 French volunteers aged 18–74 years recruited during 2009–2010 in the NutriNet-Santé Study, a general population-based e-cohort study. The Etude Nationale Nutrition Santé (ENNS; 2006–2007), a cross-sectional study with a nationally representative sample of 2,754 French adults aged 18–74 years, served as the reference data set. Reported dietary intakes from three 24-hour dietary records were weighted and compared between the two studies via Student t tests for mean location, using a &gt;5% cutoff for establishing practically meaningful differences. We observed similar intakes as regards carbohydrates, total lipids, protein, and total energy. However, intakes of fruit and vegetables, fiber, vitamins B6, B9, C, D, and E, iron, and magnesium were higher in the e-cohort than in the ENNS, while intakes of alcohol and nonalcoholic beverages were lower in the e-cohort. Significant sex-specific differences were observed regarding vitamins A and B12, zinc, and potassium. True intake differences, mode effects, and volunteer bias might each contribute to explaining the findings. In the future, repeated use of the same tool in large e-cohorts with heterogeneous dietary exposures could serve research purposes and supplement group-level monitoring of dietary trends.
Invited Commentary: Hypertension During Pregnancy and Offspring Microvascular Structure—Insights From the Retinal Microcirculation
Authors: Huckstep Odaro , Lewandowski Adam J. , Leeson Paul 
Published: October 2016

Abstract
Human clinical studies as well as laboratory animal studies demonstrate that offspring of pregnancies affected by common complications, such as preeclampsia and preterm birth, display developmental phenotypes that relate distinctly to the pregnancy disorder. Several studies have now found microvascular differences in offspring of hypertensive pregnancies, and there is interest in whether these may underlie epidemiologic associations between gestational hypertension and a higher risk of hypertension and stroke in the offspring. The retinal circulation provides a unique window into microvascular structure, of likely relevance to both the cerebrovasculature and broader cardiovascular risk. Yesil et al. (Am J Epidemiol. 2016;184(9):605–615) report in this issue of the Journal that maternal gestational blood pressure elevation is associated with reduced retinal vascular caliber in offspring at 6 years of age, providing a link between variation in pregnancy characteristics and childhood vascular development. Further work to understand the longitudinal association between pregnancy, emergence of microvascular changes, and cardiovascular risk may identify opportunities for future preventive interventions.
The Relationship Between School Holidays and Transmission of Influenza in England and Wales
Authors: Jackson Charlotte , Vynnycky Emilia , Mangtani Punam 
Published: October 2016

Abstract
School closure is often considered as an influenza control measure, but its effects on transmission are poorly understood. We used 2 approaches to estimate how school holidays affect the contact parameter (the per capita rate of contact sufficient for infection transmission) for influenza using primary care data from England and Wales (1967–2000). Firstly, we fitted an age-structured susceptible-infectious-recovered model to each year's data to estimate the proportional change in the contact parameter during school holidays as compared with termtime. Secondly, we calculated the percentage difference in the contact parameter between holidays and termtime from weekly values of the contact parameter, estimated directly from simple mass-action models. Estimates were combined using random-effects meta-analysis, where appropriate. From fitting to the data, the difference in the contact parameter among children aged 5–14 years during holidays as compared with termtime ranged from a 36% reduction to a 17% increase; estimates were too heterogeneous for meta-analysis. Based on the simple mass-action model, the contact parameter was 17% (95% confidence interval: 10, 25) lower during holidays than during termtime. Results were robust to the assumed proportions of infections that were reported and individuals who were susceptible when the influenza season started. We conclude that school closure may reduce transmission during influenza outbreaks.
Comparative analysis of recurrent events after presentation with an index myocardial infarction or ischaemic stroke
Authors: Yeo Khung Keong , Zheng Huili , Chow Khuan Yew , Ahmad Aftab , Chan Bernard P.L. , Chang Hui Meng , Chong Eric , Chua Terrance Siang Jin , Foo David Chee Guan , Low Lip Ping , Ong Marcus Eng Hock , Ong Hean Yee , Koh Tian Hai , Tan Huay Cheem , Tang Kok Foo , Venketasubramanian Narayanaswamy 
Published: October 2016

Abstract

Aims
Acute myocardial infarction (AMI) and stroke are important causes of mortality and morbidity. Our aims are to determine the comparative epidemiology of AMI and ischaemic stroke; and examine the differences in cardiovascular outcomes or mortality occurring after an AMI or stroke.

Methods and results
The Singapore National Registry of Diseases Office collects countrywide data on AMI, stroke, and mortality. Index events of AMI and ischaemic stroke between 2007 and 2012 were identified. Patients were then matched for occurrences of subsequent AMI, stroke, or death within 1-year of the index event. There were 33 222 patients with first-ever AMI and 20 982 with first-ever stroke. AMI patients were significantly more likely to be men (66.3% vs. 56.9%), non-Chinese (32.1% vs. 24.1%), and smokers (43.1% vs. 38.6%), but less likely to have hypertension (65.6% vs. 79%) and hyperlipidaemia (61.1% vs. 65.5%), compared with stroke patients. In total 6.8% of the AMI patients had recurrent AMI, whereas 4.8% of the stroke patients had recurrent stroke within 1 year; 31.7% of the AMI patients died, whereas 17.1% of the ischaemic stroke patients died within 1 year. Older age, Malay ethnicity, and diabetes mellitus were statistically significant risk factors for all-cause mortality and for the composite endpoint of AMI, stroke, and all-cause mortality, at 1 year.

Conclusions
Risk profiles of patients with AMI and stroke are significantly different. Patients suffer recurrent events in vascular territories similar to the index event. Age and diabetes mellitus are significant predictors of recurrent vascular events and mortality.
How to Pitch an Antibiotic Stewardship Program to the Hospital C-Suite
Authors: Spellberg Brad , Bartlett John G. , Gilbert David N. 
Published: October 2016

AbstractHospitals will soon require antibiotic stewardship programs. Infectious diseases specialists must craft business plans to engage hospital leadership to fund such programs. In this article, we review key cost and revenue elements that should be covered in such plans.Society is placing increasing emphasis on the importance of antimicrobial stewardship programs (ASPs). New regulatory standards require hospitals to implement ASPs. Infectious Diseases (ID) specialists will need to help design and implement such programs at hospitals. A critical component of establishing such programs is submitting a business plan to hospital leadership justifying the cost and structure of the ASP and explaining what benefits the hospital will gain in return. In this article, we explore typical elements of such business plans and describe how hospital leadership may evaluate and determine the value of such plans. Understanding hospital costs and revenue models is critical to creating a viable and realistic business plan to support ASPs.
Parasite Clearance and Artemether Pharmacokinetics Parameters Over the Course of Artemether-Lumefantrine Treatment for Malaria in Human Immunodeficiency Virus (HIV)-Infected and HIV-Uninfected Ugandan Children
Authors: Kajubi Richard , Huang Liusheng , Were Moses , Kiconco Sylvia , Li Fangyong , Marzan Florence , Gingrich David , Nyunt Myaing M. , Ssebuliba Joshua , Mwebaza Norah , Aweeka Francesca T. , Parikh Sunil 
Published: October 2016

Abstract

Background
Artemisinins are primarily responsible for initial parasite clearance. Antimalarial pharmacokinetics (PK), human immunodeficiency virus (HIV) infection, and antiretroviral therapy have been shown to impact treatment outcomes, although their impact on early parasite clearance in children has not been well characterized.

Methods
Parasite clearance parameters were generated from twice-daily blood smears in HIV-infected and HIV-uninfected Ugandan children treated with artemether-lumefantrine (AL). Artemether and dihydroartemisinin (DHA) area-under-the-curve from 0–8 hours (AUC0-8hr) after the 1st AL dose was compared with AUC0-8hr after the last (6th) dose in a concurrently enrolled cohort. The association between post-1st dose artemisinin AUC0-8hr and parasite clearance was assessed.

Results
Parasite clearance was longer in HIV-infected versus HIV-uninfected children (median, 3.5 vs 2.8 hours; P = .003). Artemether AUC0-8hr was 3- to 4-fold lower after the 6th dose versus the 1st dose of AL in HIV-infected children on nevirapine- or lopinavir/ritionavir-based regimens and in HIV-uninfected children (P ≤ .002, 1st vs 6th-dose comparisons). Children on efavirenz exhibited combined post-1st dose artemether/DHA exposure that was significantly lower than those on lopinavir/ritonavir and HIV-uninfected children. Multiple regression analysis supported that the effect of artemether/DHA exposure on parasite clearance was significantly moderated by HIV status.

Conclusions
Parasite clearance rates remain rapid in Uganda and were not found to associate with PK exposure. However, significant decreases in artemisinin PK with repeated dosing in nearly all children, coupled with small, but significant increase in parasite clearance half-life in those with HIV, may have important implications for AL efficacy, particularly because reports of artemisinin resistance are increasing.
Increased Age-Dependent Risk of Death Associated With lukF-PV-Positive Staphylococcus aureus Bacteremia
Authors: Knudsen Trine A. , Skov Robert , Petersen Andreas , Larsen Anders R. , Benfield Thomas ,   for the Danish Staphylococcal Bacteremia Study Group
Published: October 2016

Abstract

Background
Panton-Valentine leucocidin is a Staphylococcus aureus virulence factor encoded by lukF-PV and lukS-PV that is infrequent in S aureus bacteremia (SAB), and, therefore, little is known about risk factors and outcome of lukF-PV/lukS-PV-positive SAB.

Methods
This report is a register-based nationwide observational cohort study. lukF-PV was detected by polymerase chain reaction. Factors associated with the presence of lukF-PV were assessed by logistic regression analysis. Adjusted 30-day hazard ratios of mortality associated with lukF-PV status were computed by Cox proportional hazards regression analysis.

Results
Of 9490 SAB cases, 129 were lukF-PV-positive (1.4%), representing 14 different clonal complexes. lukF-PV was associated with younger age, absence of comorbidity, and methicillin-resistant S aureus. In unadjusted analysis, mortality associated with lukF-PV-positive SAB was comparable to SAB. However, lukF-PV-positive SAB nonsurvivors were significantly older and had more comorbidity. Consequently, by adjusted analysis, the risk of 30-day mortality was increased by 70% for lukF-PV-positive SAB compared with SAB (hazard ratio, 1.70; 95% confidence interval, 1.20–2.42; P = .003).

Conclusions
lukF-PV-positive SAB is rare in Denmark but associated with a significantly increased risk of mortality. Although the risk of lukF-PV-positive SAB was highest in the younger age groups, &gt;80% of deaths associated with lukF-PV-positive SAB occurred in individuals older than 55 years.
Bacterial Colonization and Antibiotic Resistance in a Prospective Cohort of Newborn Infants During the First Year of Life
Authors: Meropol Sharon B. , Stange Kurt C. , Jacobs Michael R. , Weiss Judith K. , Bajaksouzian Saralee , Bonomo Robert A. 
Published: October 2016

AbstractBackgroundInfants are virtually sterile at birth and frequently use antibiotics; our objective was to (1) characterize the longitudinal colonization with bacterial pathogens and associated antibiotic resistance in a cohort of community-dwelling infants in Northeast Ohio and (2) describe longitudinal concurrent antibiotic and daycare exposures.MethodsFor 35 newborns, nasopharyngeal swabs were cultured for Streptococcus pneumoniae, anterior nasal for Staphylococcus aureus, and perirectal for extended-spectrum beta-lactamase (ESBL)-producing Gram-negative enteric bacteria, at 3-month intervals for 12 months. Infant and household antibiotics and daycare exposure were assessed longitudinally.ResultsThirteen infants received perinatal or nursery antibiotics. By 3 months, at least 22 were colonized with Gram-negative bacteria; 2 with S pneumoniae (type 19A, resistant; 15C, susceptible), 5 with methicillin-susceptible S aureus. By 12 months, at least 22 of 35 infants received antibiotics, 20 had household members with antibiotics, and 12 attended daycare; 7 more had household members with daycare exposure. The ESBL-producing organisms were not identified. At least 10 infants were colonized at some time with an antibiotic-resistant organism, 3 more with pathogens displaying intermediate resistance. Pathogen colonization and resistance were intermittent and inconsistent.ConclusionsIn a community-based cohort followed from birth, early antibiotic and daycare exposures are common, especially considering perinatal maternal exposures. Colonization patterns of Gram-negative bacteria, S pneumoniae, S aureus, and resistant pneumococci are strikingly dynamic. Further research can identify key areas for potential interventions to maximize clinical antibiotic outcomes while minimizing future resistance.