bluuebunny/crossref_metadata_2025_split huggingface dataset:
DOCA‐salt hypertension impairs artery function in rat middle cerebral artery and parenchymal arterioles
Authors: Matin Nusrat , Pires Paulo W. , Garver Hannah , Jackson William F. , Dorrance Anne M. 
Published: September 2016

AbstractObjectiveChronic hypertension induces detrimental changes in the structure and function of surface cerebral arteries. Very little is known about PAs, which perfuse distinct neuronal populations in the cortex and may play a role in cerebrovascular disorders. We investigated the effect of DOCA‐salt induced hypertension on endothelial function and artery structure in PAs and MCAs.MethodsUninephrectomized male Sprague–Dawley rats were implanted with a subcutaneous pellet containing DOCA (150 mg/kg b.w.) and drank salt water (1% NaCl and 0.2% KCl) for 4 weeks. Sham rats were uninephrectomized and drank tap water. Vasoreactivity and passive structure in the MCAs and the PAs were assessed by pressure myography.ResultsBoth MCAs and PAs from DOCA‐salt rats exhibited impaired endothelium‐dependent dilation (P<.05). In the PAs, addition of NO and COX inhibitors enhanced dilation in DOCA‐salt rats (P<.05), suggesting that dysfunctional NO and COX‐dependent signaling could contribute to impaired endothelium‐mediated dilation. MCAs from DOCA‐salt rats exhibited inward remodeling (P<.05).ConclusionsHypertension‐induced MCA remodeling coupled with impaired endothelium‐dependent dilation in both the MCAs and PAs may exacerbate the risk of cerebrovascular accidents and the associated morbidity and mortality.
Novel allelic variants in ACD6 cause hybrid necrosis in local collection of Arabidopsis thaliana
Authors: Świadek Magdalena , Proost Sebastian , Sieh Daniela , Yu Jing , Todesco Marco , Jorzig Christian , Rodriguez Cubillos Andrés Eduardo , Plötner Björn , Nikoloski Zoran , Chae Eunyoung , Giavalisco Patrick , Fischer Axel , Schröder Florian , Kim Sang‐Tae , Weigel Detlef , Laitinen Roosa A. E. 
Published: September 2016

Summary

Hybrid necrosis is a common type of hybrid incompatibility in plants. This phenomenon is caused by deleterious epistatic interactions, resulting in spontaneous activation of plant defenses associated with leaf necrosis, stunted growth and reduced fertility in hybrids. Specific combinations of alleles of ACCELERATED CELL DEATH 6 (ACD6) have been shown to be a common cause of hybrid necrosis in Arabidopsis thaliana. Increased ACD6 activity confers broad‐spectrum resistance against biotrophic pathogens but reduces biomass production.

We generated 996 crosses among individuals derived from a single collection area around Tübingen (Germany) and screened them for hybrid necrosis. Necrotic hybrids were further investigated by genetic linkage, amiRNA silencing, genomic complementation and metabolic profiling. Restriction site associated DNA (RAD)‐sequencing was used to understand genetic diversity in the collection sites containing necrosis‐inducing alleles.

Novel combinations of ACD6 alleles found in neighbouring stands were found to activate the A. thaliana immune system. In contrast to what we observed in controlled conditions, necrotic hybrids did not show reduced fitness in the field. Metabolic profiling revealed changes associated with the activation of the immune system in ACD6‐dependent hybrid necrosis.

This study expands our current understanding of the active role of ACD6 in mediating trade‐offs between defense responses and growth in A. thaliana.
Regeneration patterns, environmental filtering and tree species coexistence in a temperate forest
Authors: Lusk Christopher H. , Laughlin Daniel C. 
Published: September 2016

Summary

Forest ecologists researching the functional basis of tree regeneration patterns and species coexistence often attempt to correlate traits with light‐gradient partitioning. However, an exclusive focus on light can overlook other important drivers of forest dynamics.

We measured light, temperatures, humidity and sapling densities in each of four phases of a forest dynamic mosaic in New Zealand: shaded understoreys, tree‐fall gaps, treefern groves and clearings. We then measured leaf, wood and seed traits, as potential predictors of species’ regeneration patterns.

Saplings of 18 out of 21 species were significantly associated with one or other of the four phases, and associations were best predicted by a two‐trait model (leaf size, wood density) explaining 51% of observed variation. Species associated with treefall gaps had traits favouring light pre‐emption (large leaves, low‐density wood), whereas those establishing in clearings mostly had small leaves and dense wood, traits probably conferring resistance to the frosts and summer water deficits that saplings were exposed to there.

The dynamics of some forests cannot be explained adequately by light‐gradient partitioning through a growth vs shade tolerance tradeoff, underpinned by the leaf economics spectrum. Consideration of multiple environmental filters and multiple traits will enhance understanding of regeneration patterns and species coexistence.
Continuous CD34+ cell collection by a new device is safe and more efficient than by a standard collection procedure: results of a two‐center, crossover, randomized trial
Authors: Cancelas Jose A. , Scott Edward P. , Bill Jerome R. 
Published: September 2016

BACKGROUNDPeripheral blood stem cells (PBSCs) collected from granulocyte–colony‐stimulating factor (G‐CSF)‐mobilized donors are routinely used for hematopoietic stem cell transplantation. Most PBSC collections worldwide have used the COBE Spectra (COBE) platform that is being replaced by the Spectra Optia (OPTIA). This study compared the PBSC collection performance and safety of the OPTIA using a single‐step, continuous mononuclear cell (CMNC) collection procedure and the standard COBE MNC procedure.STUDY DESIGN AND METHODSA prospective, noninferiority, randomized, open‐label, crossover, multicenter study was conducted in G‐CSF–mobilized donors randomized to undergo MNC collection on Days 5 and 6. The primary endpoint was CD34+ cell collection efficiency (CE1%) with a noninferiority margin of 10%. The secondary endpoint was CD34+ cell CE2%. Product purity and safety were also assessed.RESULTSTwenty‐three healthy donors (87% male) participated in the study. Mean (±SD) CD34+ CE1% was 84.4% (±16.4%) and 66.2% (±15.3%) for the OPTIA and COBE, respectively (p < 0.001 for noninferiority and superiority). Mean (±SD) CD34+ CE2% was 62.4 (±11.6) and 48.4 (±11.2) for the OPTIA and COBE, respectively (p < 0.001 for superiority). Granulocyte and platelet (PLT) contamination were lower in OPTIA‐collected products. There were no unexpected adverse events (AEs) and no significant differences in the incidence of AEs between study arms. PLT loss was less with the OPTIA than with the COBE.CONCLUSIONThe OPTIA CMNC collection procedure is safe and effective for the collection of CD34+ cells in G‐CSF–mobilized donors and was superior to the COBE for CE1% and CE2%, collecting approximately 19 and 16% higher, respectively.
Post‐authorization safety study of Clottafact®, a triply secured fibrinogen concentrate in congenital afibrinogenemia. A prospective observational study
Authors: Négrier C. , Rothschild C. , Borg J.‐Y. , Lambert T. , Claeyssens S. , Sanhes L. , Stieltjes N. , Bertrand A. , André M.‐H. , Sié P. , Gruel Y. , Tellier Z. 
Published: September 2016

Background and ObjectivesA new fibrinogen concentrate Clottafact® was developed according to European guidelines on plasma‐derived products. A post‐authorization safety study was set up in 2009 as part of the risk management plan. This was a non‐interventional, prospective, non‐comparative, multicenter study of the use of fibrinogen concentrate for congenital afibrinogenemia in real‐life medical practice in France.Materials and MethodsThe analysis was descriptive and performed on 3 subgroups: prophylaxis vs. on‐demand treatment, age (<6, <12 and ≥12) and severity of the deficiency.ResultsFourteen patients [1–78 years] were included in 7 centres and followed for 1 year. Twenty‐one adverse drug reactions (ADRs) classically reported with fibrinogen (pallor, chills, cough, vomiting, headache, urticaria and erythematous rash) were reported in 5 of 14 patients. Two ADRs were serious: an anaphylactic shock and a subclavian venous thrombosis with a favourable outcome without sequelae. In the nine patients under prophylaxis, 365 of 367 infusions were considered as successful (99·5%) and 2 as failures. For the five patients treated on‐demand, the efficacy was rated as excellent for 27 of 48 infusions and good for the 21 others.ConclusionThis study confirms that the benefit/risk balance for this fibrinogen concentrate is favourable.
Development of an excitatory kisspeptin projection to the oxytocin system in late pregnancy
Authors: Seymour Alexander J. , Scott Victoria , Augustine Rachael A. , Bouwer Gregory T. , Campbell Rebecca E. , Brown Colin H. 
Published: September 2016

Key points
Oxytocin release from the posterior pituitary gland stimulates uterine contraction during birth but the central mechanisms that activate oxytocin neurones for birth are not well characterized.
We found that that kisspeptin fibre density around oxytocin neurones increases in late‐pregnant rats.
These kisspeptin fibres originated from hypothalamic periventricular nucleus neurones that upregulated kisspeptin expression in late pregnancy.
Oxytocin neurones were excited by central kisspeptin administration in late‐pregnant rats but not in non‐pregnant rats or early‐ to mid‐pregnant rats.
Our results reveal the emergence of a new excitatory kisspeptin projection to the oxytocin system in late pregnancy that might contribute to oxytocin neurone activation for birth.
AbstractThe hormone oxytocin promotes uterine contraction during parturition. Oxytocin is synthesized by magnocellular neurones in the hypothalamic supraoptic and paraventricular nuclei and is released into the circulation from the posterior pituitary gland in response to action potential firing. Systemic kisspeptin administration increases oxytocin neurone activity to elevate plasma oxytocin levels. Here, immunohistochemistry revealed that rats on the expected day of parturition (day 21 of gestation) had a higher density of kisspeptin‐positive fibres in the perinuclear zone surrounding the supraoptic nucleus (which provides dense glutamatergic and GABAergic innervation to the supraoptic nucleus) than was evident in non‐pregnant rats. Retrograde tracing showed the kisspeptin projections to the perinuclear zone originated from the hypothalamic periventricular nucleus. Quantitative RT‐PCR showed that kisspeptin receptor mRNA, Kiss1R mRNA, was expressed in the perinuclear zone–supraoptic nucleus and that the relative Kiss1R mRNA expression does not change over the course of pregnancy. Finally, intracerebroventricular administration of kisspeptin increased the firing rate of oxytocin neurones in anaesthetized late‐pregnant rats (days 18–21 of gestation) but not in non‐pregnant rats, or in early‐ or mid‐pregnant rats. Taken together, these results suggest that kisspeptin expression is upregulated in the periventricular nucleus projection to the perinuclear zone of the supraoptic nucleus towards the end of pregnancy. Hence, this input might activate oxytocin neurones during parturition.
Visión panorámica de la representación de objetos en la literatura hispánica reciente
Authors: Luis Mora Vicente 
Published: September 2016

<p>En la última literatura hispánica hay varias para posibilidades dirigidas a construir objetos ontológicos. En las actuales narrativa y poesía no es difícil establecer dos categorías de fundación ontológica: las cosas <em>auráticas </em>y las proyectadas, con diferentes significados y consecuencias, que serán discutidas en el texto. </p>
First Genome Sequences of Porcine Parvovirus 5 Strains Identified in Polish Pigs
Authors: Fan Jinghui , Cui Jin , Gerber Priscilla F. , Biernacka Kinga , Stadejek Tomasz , Opriessnig Tanja 
Published: September 2016

ABSTRACT
Porcine parvovirus type 5 (PPV5) has been recently identified. Here, we report the genome sequences of five PPV5 strains identified in serum samples from Polish pigs, which represent the first PPV5 sequences recovered from European pigs. The PPV5 strains isolated in Poland are most related to the Chinese strain HN01.
Complete Genome Sequences of Four Enterohemolysin-Positive (
ehxA
) Enterocyte Effacement-Negative Shiga Toxin-Producing Escherichia coli Strains
Authors: Lorenz Sandra C. , Kotewicz Michael L. , Hoffmann Maria , Gonzalez-Escalona Narjol , Fischer Markus , Kase Julie A. 
Published: September 2016

ABSTRACT

Shiga toxin-producing
Escherichia coli
(STEC) strains are important foodborne pathogens associated with human disease. Most disease-associated STEC strains carry the locus of enterocyte effacement (LEE); however, regularly LEE-negative STEC strains are recovered from ill patients. Few reference sequences are available for these isolate types. Here, we report here the complete genome sequences for four LEE-negative STEC strains.
High-Quality Draft Genome Sequences of Two
Xanthomonas
Pathotype Strains Infecting Aroid Plants
Authors: Robène I. , Bolot S. , Pruvost O. , Arlat M. , Noël L. D. , Carrère S. , Jacques M.-A. , Koebnik R. , Gagnevin L. 
Published: September 2016

ABSTRACT

We present here the draft genome sequences of bacterial pathogens of the
Araceae
family,
Xanthomonas axonopodis
pv. dieffenbachiae LMG 695 and
Xanthomonas campestris
pv. syngonii LMG 9055, differing in host range. A comparison between genome sequences will help understand the mechanisms involved in tissue specificity and adaptation to host plants.
Complete Genome Sequence of the
d
-Amino Acid Catabolism Bacterium
Phaeobacter
sp. Strain JL2886, Isolated from Deep Seawater of the South China Sea
Authors: Fu Yingnan , Wang Rui , Zhang Zilian , Jiao Nianzhi 
Published: September 2016

ABSTRACT

Phaeobacter
sp. strain JL2886, isolated from deep seawater of the South China Sea, can catabolize
d
-amino acids. Here, we report the complete genome sequence of
Phaeobacter
sp. JL2886. It comprises ~4.06 Mbp, with a G+C content of 61.52%. A total of 3,913 protein-coding genes and 10 genes related to
d
-amino acid catabolism were obtained.
Genome Sequence of a Candidate World Health Organization Reference Strain of Zika Virus for Nucleic Acid Testing
Authors: Trösemeier Jan-Hendrik , Musso Didier , Blümel Johannes , Thézé Julien , Pybus Oliver G. , Baylis Sally A. 
Published: September 2016

ABSTRACT
We report here the sequence of a candidate reference strain of Zika virus (ZIKV) developed on behalf of the World Health Organization (WHO). The ZIKV reference strain is intended for use in nucleic acid amplification (NAT)-based assays for the detection and quantification of ZIKV RNA.
Genome Sequence of Aeromonas hydrophila Strain AH-3 (Serotype O34)
Authors: Forn-Cuní Gabriel , Tomás Juan M. , Merino Susana 
Published: September 2016

ABSTRACT

Aeromonas hydrophila
is an emerging pathogen of poikilothermic animals, from fish to mammals, including humans. Here, we report the whole-genome sequence of the
A. hydrophila
AH-3 strain, isolated from a fish farm goldfish septicemia outbreak in Spain, with a characterized polar and lateral flagellum glycosylation pattern.
Draft Genome Sequence of the Probiotic Yeast Kluyveromyces marxianus
fragilis
B0399
Authors: Quarella Sara , Lovrovich Paola , Scalabrin Simone , Campedelli Ilenia , Backovic Ana , Gatto Veronica , Cattonaro Federica , Turello Alessandro , Torriani Sandra , Felis Giovanna E. 
Published: September 2016

ABSTRACT

Here, we report the draft genome sequence of
Kluyveromyces marxianus
fragilis
B0399, the first yeast approved as a probiotic for human consumption not belonging to the genus
Saccharomyces
. The genome is composed of 8 chromosomes, with a total size of 11.44 Mb, including mitochondrial DNA.
Full-Genome Sequence of Escherichia coli K-15KW01, a Uropathogenic E. coli B2 Sequence Type 127 Isolate Harboring a Chromosomally Carried
bla
CTX-M-15
Gene
Authors: Zurfluh Katrin , Tasara Taurai , Stephan Roger 
Published: September 2016

ABSTRACT

We present here the full-genome sequence of
Escherichia coli
K-15KW01, an extended-spectrum-β-lactamase-producing uropathogenic strain. Assembly and annotation of the draft genome resulted in a 5,154,641-bp chromosome and revealed a chromosomally contained
bla
CTX-M-15
gene embedded at the right-hand extremity of an IS
Ecp1
element in a plasmid-like structure (36,907 bp).
Myocardial infarction, ST-elevation and non-ST-elevation myocardial infarction and modelled daily pollution concentrations: a case-crossover analysis of MINAP data
Authors: Butland Barbara K , Atkinson Richard W , Milojevic Ai , Heal Mathew R , Doherty Ruth M , Armstrong Ben G , MacKenzie Ian A , Vieno Massimo , Lin Chun , Wilkinson Paul 
Published: September 2016

ObjectivesTo investigate associations between daily concentrations of air pollution and myocardial infarction (MI), ST-elevation MI (STEMI) and non-ST-elevation MI (NSTEMI).MethodsModelled daily ground-level gaseous, total and speciated particulate pollutant concentrations and ground-level daily mean temperature, all at 5 km×5 km horizontal resolution, were linked to 202 550 STEMI and 322 198 NSTEMI events recorded on the England and Wales Myocardial Ischaemia National Audit Project (MINAP) database. The study period was 2003–2010. A case-crossover design was used, stratified by year, month and day of the week. Data were analysed using conditional logistic regression, with pollutants modelled as unconstrained distributed lags 0–2 days. Results are presented as percentage change in risk per 10 µg/m3increase in the pollutant relevant metric, having adjusted for daily mean temperature, public holidays, weekly influenza consultation rates and a sine-cosine annual cycle.ResultsThere was no evidence of an association between MI or STEMI and any of O3, NO2, PM2.5, PM10or selected PM2.5components (sulfate and elemental carbon). For NSTEMI, there was a positive association with daily maximum 1-hour NO2(0.27% (95% CI 0.01% to 0.54%)), which persisted following adjustment for O3and adjustment for PM2.5. The association appeared to be confined to the midland and southern regions of England and Wales.ConclusionsThe study found no evidence of an association between the modelled pollutants (including components) investigated and STEMI but did find some evidence of a positive association between NO2and NSTEMI. Confirmation of this association in other studies is required.
Neutrino masses in the SU(4)L ⊗ U(1)X electroweak extension of the Standard Model
Authors: Palacio Guillermo 
Published: September 2016

We study the neutrino mass generation in the [Formula: see text] electroweak extension of the Standard Model by considering nonrenormalizable dimension 5 effective operators. It is shown that there exist two topologies for the realizations of such an operator at the tree-level and for one of the three-family models the neutrino phenomenology is explored after extending its particle content with an [Formula: see text] fermion singlet and a scalar decuplet. Constraints in the available parameters space of the model are partially discussed.
Humanized Mouse Xenograft Models: Narrowing the Tumor–Microenvironment Gap
Authors: Morton J. Jason , Bird Gregory , Refaeli Yosef , Jimeno Antonio 
Published: September 2016

Abstract
Cancer research has long been hampered by the limitations of the current model systems. Both cultured cells and mouse xenografts grow in an environment highly dissimilar to that of their originating tumor, frequently resulting in promising treatments that are ultimately clinically ineffective. The development of highly immunodeficient mouse strains into which human immune systems can be engrafted can help bridge this gap. Humanized mice (HM) allow researchers to examine xenograft growth in the context of a human immune system and resultant tumor microenvironment, and recent studies have highlighted the increased similarities in attendant tumor structure, metastasis, and signaling to those features in cancer patients. This setting also facilitates the examination of investigational cancer therapies, including new immunotherapies. This review discusses recent advancements in the generation and application of HM models, their promise in cancer research, and their potential in generating clinically relevant treatments. This review also focuses on current efforts to improve HM models by engineering mouse strains expressing human cytokines or HLA proteins and implanting human bone, liver, and thymus tissue to facilitate immune cell maturation and trafficking. Finally, we discuss how these improvements may help direct future HM model cancer studies. Cancer Res; 76(21); 6153–8. ©2016 AACR.
Changes in Chromatin Structure in Curettage Specimens Identifies High-Risk Patients in Endometrial Cancer
Authors: Hveem Tarjei S. , Njølstad Tormund S. , Nielsen Birgitte , Syvertsen Rolf Anders , Nesheim John Arne , Kjæreng Marna L. , Kildal Wanja , Pradhan Manohar , Marcickiewicz Janusz , Tingulstad Solveig , Staff Anne C. , Haugland Hans K. , Eraker Runar , Oddenes Klaus , Rokne Jan A. , Tjugum Jostein , Lode Margaret S. , Amant Frederic , Werner Henrica M.J. , Bjørge Line , Albregtsen Fritz , Liestøl Knut , Salvesen Helga B. , Trovik Jone , Danielsen Håvard E. 
Published: September 2016

Abstract
Background: Most endometrial carcinoma patients are diagnosed at an early stage with a good prognosis. However, a relatively low fraction with lethal disease constitutes a substantial number of patients due to the high incidence rate. Preoperative identification of patients with high risk and low risk for poor outcome is necessary to tailor treatment. Nucleotyping refers to characterization of cell nuclei by image cytometry, including the assessment of chromatin structure by nuclear texture analysis. This method is a strong prognostic marker in many cancers but has not been evaluated in preoperative curettage specimens from endometrial carcinoma.
Methods: The prognostic impact of changes in chromatin structure quantified with Nucleotyping was evaluated in preoperative curettage specimens from 791 endometrial carcinoma patients prospectively included in the MoMaTEC multicenter trial.
Results: Nucleotyping was an independent prognostic marker of disease-specific survival in preoperative curettage specimens among patients with Federation Internationale des Gynaecologistes et Obstetristes (FIGO) stage I–II disease (HR=2.9; 95% CI, 1.2–6.5; P = 0.013) and significantly associated with age, FIGO stage, histologic type, histologic grade, myometrial infiltration, lymph node status, curettage histology type, and DNA ploidy.
Conclusions: Nucleotyping in preoperative curettage specimens is an independent prognostic marker for disease-specific survival, with potential to supplement existing parameters for risk stratification to tailor treatment.
Impact: This is the first study to evaluate the prognostic impact of Nucleotyping in curettage specimens from endometrial carcinoma and shows that this may be a clinically useful prognostic marker in endometrial cancer. External validation is warranted. Cancer Epidemiol Biomarkers Prev; 26(1); 61–67. ©2016 AACR.