bluuebunny/crossref_metadata_2025_split huggingface dataset:
Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
Authors: Ohira Suguru , Tanaka Hideo , Harada Yoshinori , Minamikawa Takeo , Kumamoto Yasuaki , Matoba Satoaki , Yaku Hitoshi , Takamatsu Tetsuro 
Published: February 2017

AbstractRaman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy is applicable for evaluating myocardial ischaemia especially during early ischaemic phase. We obtained spontaneous Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart upon 532-nm excitation before and during the “stopped-flow,” global ischaemia. Semi-quantitative values of the peak intensities at 750 and 1127 cm−1, which reflect reduced cytochromes c and b, increased immediately and progressively after induction of the stopped flow, indicating progressive reduction of the mitochondrial respiration. Such spectral changes emerged before the loss of 1) mitochondrial membrane potentials measured by the fluorescence intensity of tetramethyl rhodamine ethyl ester or 2) staining of the triphenyl tetrazolium chloride dye in the myocardium. The progressive increases in the Raman peaks by stopped flow were significantly retarded by ischaemic preconditioning. Sequential measurements of the peak intensities at 750 and 1127 cm−1 enabled early detection of the myocardial ischaemia based on the mitochondrial functions. These data suggest that Raman spectroscopy offers the potential to evaluate acute ischaemic heart under label-free conditions.
Optical Measurement of In-plane Waves in Mechanical Metamaterials Through Digital Image Correlation
Authors: Schaeffer Marshall , Trainiti Giuseppe , Ruzzene Massimo 
Published: February 2017

AbstractWe report on a Digital Image Correlation-based technique for the detection of in-plane elastic waves propagating in structural lattices. The experimental characterization of wave motion in lattice structures is currently of great interest due its relevance to the design of novel mechanical metamaterials with unique/unusual properties such as strongly directional behaviour, negative refractive indexes and topologically protected wave motion. Assessment of these functionalities often requires the detection of highly spatially resolved in-plane wavefields, which for reticulated or porous structural assemblies is an open challenge. A Digital Image Correlation approach is implemented that tracks small displacements of the lattice nodes by centring image subsets about the lattice intersections. A high speed camera records the motion of the points by properly interleaving subse- quent frames thus artificially enhancing the available sampling rate. This, along with an imaging stitching procedure, enables the capturing of a field of view that is sufficiently large for subsequent processing. The transient response is recorded in the form of the full wavefields, which are processed to unveil features of wave motion in a hexagonal lattice. Time snapshots and frequency contours in the spatial Fourier domain are compared with numerical predictions to illustrate the accuracy of the recorded wavefields.
Responses of spatial-temporal dynamics of bacterioplankton community to large-scale reservoir operation: a case study in the Three Gorges Reservoir, China
Authors: Li Zhe , Lu Lunhui , Guo Jinsong , Yang Jixiang , Zhang Jiachao , He Bin , Xu Linlin 
Published: February 2017

AbstractLarge rivers are commonly regulated by damming, yet the effects of such disruption on bacterioplankton community structures have not been adequately studied. The aim of this study was to explore the biogeographical patterns present under dam regulation and to uncover the major drivers structuring bacterioplankton communities. Bacterioplankton assemblages in the Three Gorges Reservoir (TGR) were analyzed using Illumina Miseq sequencing by comparing seven sites located within the TGR before and after impoundment. This approach revealed ecological and spatial-temporal variations in bacterioplankton community composition along the longitudinal axis. The community was dynamic and dominated by Proteobacteria and Actinobacteria phyla, encompassing 39.26% and 37.14% of all sequences, respectively, followed by Bacteroidetes (8.67%) and Cyanobacteria (3.90%). The Shannon-Wiener index of the bacterioplankton community in the flood season (August) was generally higher than that in the impoundment season (November). Principal Component Analysis of the bacterioplankton community compositions showed separation between different seasons and sampling sites. Results of the relationship between bacterioplankton community compositions and environmental variables highlighted that ecological processes of element cycling and large dam disturbances are of prime importance in driving the assemblages of riverine bacterioplankton communities.
Plasmon-Enhanced Photocurrent using Gold Nanoparticles on a Three-Dimensional TiO2 Nanowire-Web Electrode
Authors: Yen Yin-Cheng , Chen Jau-An , Ou Sheng , Chen Yi-Shin , Lin Kuan-Jiuh 
Published: February 2017

AbstractIn this study, an anatase/rutile mixed-phase titanium dioxide (TiO2) hierarchical network deposited with Au nanoparticles (Au/TiO2 ARHN) was synthesized using a facile hydrothermal method followed by a simple calcination step. Such a unique structure was designed for improving the light harvest, charge transportation/separation, and the performance of photo-electro-chemical (PEC) cells. The properties of the as-synthesized Au/TiO2 ARHN in PEC cells were investigated by electrochemical measurements using a three-electrode system in a 1 M NaOH electrolyte. Remarkably, a 4.5-folds enhancement of the photocurrent for Au/TiO2 ARHN was observed as compared to that for TiO2 nanowire (NW), under AM1.5G solar illumination, suggesting its potential application in PEC cells. A mechanism has been proposed to explain the high photocurrent of Au/TiO2 ARHN in PEC water splitting.
Evaluation of Acridine Orange Derivatives as DNA-Targeted Radiopharmaceuticals for Auger Therapy: Influence of the Radionuclide and Distance to DNA
Authors: Pereira Edgar , do Quental Letícia , Palma Elisa , Oliveira Maria Cristina , Mendes Filipa , Raposinho Paula , Correia Isabel , Lavrado João , Di Maria Salvatore , Belchior Ana , Vaz Pedro , Santos Isabel , Paulo António 
Published: February 2017

AbstractA new family of 99mTc(I)- tricarbonyl complexes and 125I-heteroaromatic compounds bearing an acridine orange (AO) DNA targeting unit was evaluated for Auger therapy. Characterization of the DNA interaction, performed with the non-radioactive Re and 127I congeners, confirmed that all compounds act as DNA intercalators. Both classes of compounds induce double strand breaks (DSB) in plasmid DNA but the extent of DNA damage is strongly dependent on the linker between the Auger emitter (99mTc or 125I) and the AO moiety. The in vitro evaluation was complemented with molecular docking studies and Monte Carlo simulations of the energy deposited at the nanometric scale, which corroborated the experimental data. Two of the tested compounds, 125I-C5 and 99mTc-C3, place the corresponding radionuclide at similar distances to DNA and produce comparable DSB yields in plasmid and cellular DNA. These results provide the first evidence that 99mTc can induce DNA damage with similar efficiency to that of 125I, when both are positioned at comparable distances to the double helix. Furthermore, the high nuclear retention of 99mTc-C3 in tumoral cells suggests that 99mTc-labelled AO derivatives are more promising for the design of Auger-emitting radiopharmaceuticals than the 125I-labelled congeners.
12‐Channel, 480 Gbit/s optical receiver analogue front‐end in 0.13 μm BiCMOS technology
Authors: Chen Yingmei , Li Jiquan , Zhang Zhen , Wang Hui , Zhang Yunan 
Published: February 2017

A compact 12‐channel 480 Gbit/s optical receiver analogue front‐end (AFE) for parallel optical transmission systems has been implemented in 0.13 μm SiGe BiCMOS technology. The transimpedance amplifier of AFE employed common‐gate amplifier and negative feedback structure to decrease input impedance and broaden bandwidth. The limiting amplifier adopted modified Cherry–Hooper amplifier with active feedback technique to stabilise current and acquire high gain simultaneously. Without on‐chip spiral inductors, the spacing of adjacent channels is constricted to 250 μm and the chip area is only 3350 × 700 μm. With the psuedo‐random binary sequence (PRBS) input data, the maximum operating rate of the AFE is 38.7 Gbit/s; meanwhile, the root mean square (RMS) jitter is 2.16 ps and the single‐ended output amplitude of 183.7 mV is achieved. The power consumption of each channel is 150 mW from a 3.3 V supply.
Estrone and Estradiol Levels in Breast Cancer Patients Using Anastrozole Are Not Related to Body Mass Index
Authors: Coscia Eduardo Borges , Sabha Maricene , Gerenutti Marli , Groppo Francisco Carlos , Bergamaschi Cristiane de Cássia 
Published: February 2017

Objective Obesity is associated with an increased risk for breast cancer. Recent studies have shown that aromatase inhibitors may be less effective in women with a high body mass index (BMI). The aim of this study was to establish the relationship between the BMI and plasma estrone and estradiol levels in postmenopausal women with hormone receptor-positive breast cancer using anastrozole.
Methods In this cohort study, the patients were divided into three groups according to BMI (normal weight, overweight and obese) to compare and correlate plasma hormone levels before starting anastrozole hormone therapy and three months after treatment. Plasma hormone levels were compared for age and use of chemotherapy.
Results A statistically significant reduction in estrone and estradiol levels was observed between baseline and three months after starting the anastrozole treatment (p < 0.05). There was no statistically significant difference in plasma estrone and estradiol levels among the BMI groups (p > 0.05), but a significant reduction in plasma estrone levels was observed after three-months' treatment relative to baseline in all groups, as well as a reduction in estradiol in the obese group (p < 0.05). The use of chemotherapy and age > 65 years had no influence on plasma steroid levels.
Conclusion Changes in estrone and estradiol levels in the studied groups were not associated with BMI, chemotherapy or age.
Nasalance Changes Following Various Endonasal Surgeries
Authors: Amer Hazem , Elaassar Ahmed , Anany Ahmad , Quriba Amal 
Published: February 2017

Introduction There is change in nasalance post endonasal surgery which is not permanent.
Objectives The objective of this study is to evaluate the long-term nasalance changes following different types of endonasal surgeries.
Methods We included in this study patients who underwent sinonasal surgery at the Otorhinolaryngology Department in Zagazig University Hospitals from February 2015 until March 2016. We divided the patients into two groups according to the surgeries they underwent: Group (A) was the FESS group and group (B), the septoturbinoplasty group. We checked nasalance using a nasometer before and after the sinonasal surgery.
Results Nasalance increased at one month after the operation in both groups. However, it returned to nearly original levels within three months postoperatively.
Conclusion FESS, septoplasty, and turbinate surgery may lead to hypernasal speech. This hypernasal speech can be a result of change in the shape and diameter of the resonating vocal tract. Hypernasal speech in these circumstances may be a temporary finding that can decrease with time. Surgeons should inform their patients about the possibility of hypernasality after such types of surgery, especially if they are professional voice users.
Intraoperative optische Kohärenztomografie – eine aktuelle Übersicht klinischer Studien zur Anwendung im Vorder- und Hinterabschnitt
Authors: Augustin A. 
Published: February 2017

ZusammenfassungDie intraoperative optische Kohärenztomografie (iOCT) stellt einen weiteren, erheblichen Entwicklungsschritt in der ophthalmologischen Bildgebung dar – erstmals sind nun hochaufgelöste OCT-Darstellungen nicht nur prä- und postoperativ, sondern auch intraoperativ verfügbar. In den letzten Jahren wurden intraoperative OCT-Technologien intensiv erforscht und von ersten, handgehaltenen, über auf das Mikroskop aufmontierten Systemen bis hin zu vollständig in das Operationsmikroskop integrierten Systemen weiterentwickelt. Diese liefern uns während eines chirurgischen Eingriffs bei quasi unverändertem Arbeitsumfeld in Echtzeit 3-dimensionale OCT-Bilder und bieten uns so zusätzlich zur Mikroskopansicht weitere Informationen, wie z. B. zu Mikrostrukturen der Netzhaut oder der Kornea, die die Operationsstrategie bei Vorder- und Hinterabschnittseingriffen beeinflussen können. Wie jede neue Technologie weist auch die iOCT derzeit noch einige Limitationen auf, wie z. B. durch die Instrumente hervorgerufene Abschattungen des darunterliegenden Gewebes oder fehlende Tracking-Systeme. Daher ist zum derzeitigen Stand der Technologie noch etwas Geschick erforderlich, um chirurgische Manöver mit der iOCT in Echtzeit nachzuverfolgen. Dies wird durch zukünftige Weiterentwicklungen sicherlich behoben werden. Auch wenn die iOCT nicht für alle Prozeduren erforderlich ist, so kann es doch bei vielen chirurgischen Eingriffen am Vorder- bzw. Hinterabschnitt schon heute zu mehr Sicherheit und Kontrolle beitragen, wie zahlreiche Studien und Fallserien zeigen. Insbesondere bei der Entfernung vitreomakulärer Traktionen oder epiretinaler Membranen (ERM-Peeling) wie auch bei der Operation von Makulaforamina bietet die iOCT den Chirurgen eine deutliche Unterstützung, indem sie zur besseren Visualisierung transparenter Strukturen beiträgt, eine direkte Erfolgskontrolle ermöglicht und in bestimmten Fällen den Einsatz von Farbstoffen überflüssig macht. Auch bei lamellären hornhautchirurgischen Eingriffen sowie in der Glaukomchirurgie kann die iOCT zu mehr Kontrolle und Präzision beitragen. Zudem können mithilfe der iOCT neue wissenschaftliche Erkenntnisse zu verschiedenen Pathologien gewonnen, Auswirkungen chirurgischer Manöver auf die okuläre Strukturen besser evaluiert und somit chirurgische Manöver langfristig optimiert werden. Zur besseren Beurteilung des Nutzens der iOCT für verschiedene chirurgische Prozeduren werden zukünftig weitere prospektive Studien erforderlich sein.
Epi-Bowman-Keratektomie: klinische Beurteilung einer neuen Variante der Surface Ablation
Authors: Taneri S. , Kießler S. , Rost A. , Schultz T. , Elling M. , Dick B. 
Published: February 2017

Zusammenfassung
Ziel Kürzlich wurde ein neues Instrument zur Entfernung des Hornhautepithels vor Excimer-Laser Surface Ablation oder kollagener Quervernetzung (CXL) vorgestellt, das Epi-Clear™-Keratom (Orca Surgical, Kiryat-Shmona, Israel). Wir wollten die Literatur bez. klinischer Ergebnisse und Vor- und Nachteilen im Vergleich zu anderen Methoden der Epithelabrasio analysieren.
Methode In PubMed wurden zuletzt am 09. 11. 2016 folgende Stichwörter zur Literatursuche eingegeben: „Epi-Bowman Keratectomy“, „Epi-clear“, „Epikeratome“. Weiterhin wurde online bei internationalen augenärztlichen Kongressen (European Society of Cataract and Refractive Surgeons [ESCRS] und American Society of Cataract and Refractive Surgery [ASCRS]) nach Postern und Präsentationen gesucht sowie beim Hersteller nach Veröffentlichungen angefragt. Die Literatursuche ergab 1 Peer-Review-Publikation, 1 Non-Peer-Review-Veröffentlichung, 18 themenrelevante Poster und Präsentationen über den refraktiven Einsatz des Epi-Clear-Keratoms, 2 Poster für die Anwendung vor CXL und 1 Präsentation zur experimentellen Nutzung des Epi-Clear-Keratoms zur Entfernung eines Pterygiums.
Ergebnisse Im Vergleich mit anderen bekannten Methoden der Surface Ablation, z. B. alkoholassistierte PRK oder PRK mittels Hornhautschaber, scheint die Epi-Bowman-Keratektomie™ (EBK™) mindestens gleiche, teils auch bessere Ergebnisse zu liefern. So wird nach EBK u. a. von geringeren Schmerzen, geringerer Haze-Bildung und einer kürzeren Heilungszeit berichtet. Auch sei die Erholung der Sehleistung schneller als mit anderen Methoden. Auch im Rahmen einer CXL wurde eine signifikant kürzere Heilungszeit im direkten Vergleich zur Epithelabrasio mittels Hornhautschaber und ein geringeres Schmerzempfinden der Patienten beschrieben.
Schlussfolgerung Zusammenfassend scheint das Epi-Clear-Keratom ein vielversprechendes Instrument zur Epithelabrasio vor refraktiver Laserablation zu sein. Allerdings fehlt noch eine überzeugende Erklärung zum prinzipiellen Vorteil. Außerdem scheint das Epi-Clear-Keratom besonders bei CXL Vorteile zu bieten, da hier die Bowman-Membran dauerhaft geschont wird. Dennoch sind für eine abschließende Beurteilung weitere Studien mit höherer Belastbarkeit (kontrolliert, randomisiert und in ausreichender Fallzahl) nötig.
Electrical Guidance Efficiency of Downstream‐Migrating Juvenile Sea Lampreys Decreases with Increasing Water Velocity
Authors: Miehls Scott M. , Johnson Nicholas S. , Haro Alex 
Published: February 2017

AbstractWe tested the efficacy of a vertically oriented field of pulsed direct current (VEPDC) created by an array of vertical electrodes for guiding downstream‐moving juvenile Sea Lampreys Petromyzon marinus to a bypass channel in an artificial flume at water velocities of 10–50 cm/s. Sea Lampreys were more likely to be captured in the bypass channel than in other sections of the flume regardless of electric field status (on or off) or water velocity. Additionally, Sea Lampreys were more likely to be captured in the bypass channel when the VEPDC was active; however, an interaction between the effects of VEPDC and water velocity was observed, as the likelihood of capture decreased with increases in water velocity. The distribution of Sea Lampreys shifted from right to left across the width of the flume toward the bypass channel when the VEPDC was active at water velocities less than 25 cm/s. The VEPDC appeared to have no effect on Sea Lamprey distribution in the flume at water velocities greater than 25 cm/s. We also conducted separate tests to determine the threshold at which Sea Lampreys would become paralyzed. Individuals were paralyzed at a mean power density of 37.0 µW/cm3. Future research should investigate the ability of juvenile Sea Lampreys to detect electric fields and their specific behavioral responses to electric field characteristics so as to optimize the use of this technology as a nonphysical guidance tool across variable water velocities.Received April 26, 2016; accepted November 1, 2016 Published online February 13, 2017
Solubility of soy lipophilic proteins: comparison with other soy protein fractions
Authors: Sirison Jiraporn , Matsumiya Kentaro , Samoto Masahiko , Hidaka Hiroshi , Kouno Mitsutaka , Matsumura Yasuki 
Published: February 2017

Abstract
Solubility of soy lipophilic proteins (LP) was studied as compared with that of other soy protein fractions. LP, β-conglycinin, glycinin, and soy protein isolate (N-SPI) were prepared under the condition to avoid heat denaturation. Solubility of LP was lower than that of other soy protein fractions under all the tested conditions varying in pH values and ionic strength. The solubility of LP was increased constantly by elevating temperature until 90 °C, whereas that of β-conglycinin and glycinin dropped at high temperature. Temperature-dependent change in solubility of N-SPI might reflect the balance among that of glycinin, β-conglycinin and LP. Based on the results of SDS-PAGE, determination of phospholipid content and Fourier Transform Infrared spectroscopy, we discussed the solubilization behavior of LP relating to its origin and composition.
Low frequency bi-syllabic wordlists in a South-Indian language, Kannada
Authors: Barman Animesh , Narne Vijaya Kumar , Prabhu Prashanth , Singh Niraj Kumar , Thammaiah Spoorthi 
Published: February 2017

Objective:
The present study aimed to develop, standardize and validate low frequency bi-syllabic wordlists in Kannada, a South-Indian language.

Study design:
The study was conducted in three different phases. The Kannada low frequency wordlists were developed in Phase I. The procedure involved collecting bi-syllabic familiar words, recording them, selecting the words dominant in low frequency energy by acoustical (Fast Fourier) transform and statistical means (k-means clustering) then generating equivalent wordlists using psychometric function. In Phase II, all the wordlists developed were standardized through estimation of speech identification scores in 100 individuals with normal hearing and through re-verification of equivalence of wordlists’ difficulty level by obtaining psychometric function. Finally, during Phase III, lists developed were evaluated for usefulness by administering them along with conventional phonemically-balanced Kannada wordlist on 10 individuals with cochlear hearing loss having rising audiometric configurations (i.e. more loss at lower frequencies).

Results:
Phase I resulted in development of seven psychometrically equivalent wordlists. Speech identification scores on 100 individuals with normal hearing showed mean scores greater than 95% for all the lists at 40 dB SL. No statistical difference was noted across wordlists. Further, individuals with rising cochlear hearing loss (RCHL) performed significantly poorer when compared to normal hearing counterparts across wordlists except low frequency wordlist 4 and phonemically balanced wordlist.

Conclusions:
The study utilized a unique procedure for the development of wordlists which can serve as guidelines for further research. The study has resulted in standardization (along with generation of normative data) and successful validation of the lists (except list 4) developed on a clinical population, i.e. individuals with RCHL. Given the lack of availability and the current clinical/research need of such test materials, the wordlists generated from this study can be useful.
PhosphoPICK-SNP: quantifying the effect of amino acid variants on protein phosphorylation
Authors: Patrick Ralph , Kobe Bostjan , Lê Cao Kim-Anh , Bodén Mikael 
Published: February 2017

Abstract

Motivation
Genome-wide association studies are identifying single nucleotide variants (SNVs) linked to various diseases, however the functional effect caused by these variants is often unknown. One potential functional effect, the loss or gain of protein phosphorylation sites, can be induced through variations in key amino acids that disrupt or introduce valid kinase binding patterns. Current methods for predicting the effect of SNVs on phosphorylation operate on the sequence content of reference and variant proteins. However, consideration of the amino acid sequence alone is insufficient for predicting phosphorylation change, as context factors determine kinase-substrate selection.

Results
We present here a method for quantifying the effect of SNVs on protein phosphorylation through an integrated system of motif analysis and context-based assessment of kinase targets. By predicting the effect that known variants across the proteome have on phosphorylation, we are able to use this background of proteome-wide variant effects to quantify the significance of novel variants for modifying phosphorylation. We validate our method on a manually curated set of phosphorylation change-causing variants from the primary literature, showing that the method predicts known examples of phosphorylation change at high levels of specificity. We apply our approach to data-sets of variants in phosphorylation site regions, showing that variants causing predicted phosphorylation loss are over-represented among disease-associated variants.

Availability and Implementation
The method is freely available as a web-service at the website http://bioinf.scmb.uq.edu.au/phosphopick/snp

Supplementary information
Supplementary data are available at Bioinformatics online.