bluuebunny/crossref_metadata_2025_split huggingface dataset:
Organizational learning programs to fulfilling basic needs at work: significance for work attractiveness and turnover in strained sectors
Authors: Dellve Lotta , Jonsson Robin , Arman Rebecka , Gillberg Nanna , Wikström Ewa 
Published: October 2024

Purpose
This study aims to explore whether participation in employer-provided skills and learning programs can strengthen older workers’ abilities to carry out their work in a meaningful way so that it increases work attractiveness and a willingness to remain in the current job position.

Design/methodology/approach
A survey was distributed to assistance nurses, nurses and teachers, aged 55 years and older in a Swedish City (n = 1,342), analyzed descriptively and with structural equation modeling.

Findings
This paper showed positive relationships between active participation in organizational learning programs (OLPs) and autonomy, relatedness, competence and also attractive work. Associations are observed between participation in learning programs, e.g. mentorship, through the strengthened basic needs at work with work attractiveness and lower intention to leave, but not prolonged retirement preference.

Research limitations/implications
The cross-sectional quantitative design restricts drawing causal conclusions about associations.

Practical implications
OLPs at work may be seen as potential measures to strengthen work conditions, fulfilling basic psychological needs at work and increase work attractiveness in strained welfare sectors.

Social implications
There are some welfare sectors that – more than others – are strained by challenges to maintain, sustain and develop quality, knowledge and staff due to poor economic and social resources with regard to sustainability, e.g. in the educational and caring sectors. Strengthening organizational measures is needed to support sustainable development.

Originality/value
This study applies advanced statistical methods, in a large empirical sample, and shows the importance of skills and learning programs for job attractiveness among older workers in female-dominated, strained welfare sectors.
Disparities in infectious disease‐related health care utilization between Medicaid‐enrolled American Indians and non‐Hispanic Whites—Lessons from the first 16 months of coronavirus disease 2019 and a decade of flu seasons
Authors: Wang Scarlett Sijia , Akee Randall , Simeonova Emilia , Glied Sherry 
Published: October 2024

AbstractObjectiveTo understand why American Indian and Alaskan Native (AIAN) populations have had exceptionally high COVID‐19 mortality, we compare patterns of healthcare utilization and outcomes for two serious infectious respiratory diseases—Influenza‐like‐illness (ILI) and coronavirus disease 2019 (COVID‐19)—between American Indian and Alaskan Native (AIAN) populations (as identified in Medicaid data) and non‐Hispanic Whites over the 2009–2021 period.Study Setting and DesignWe select all people under the age of 65 years identified as non‐Hispanic White or AIAN in the New York State Medicaid claims data between 2009 and 2021. We analyze data across 10 ILI cohorts (between September 2009 and August 2020) and 4 COVID‐19 cohorts (March–June 2020, July–September 2020, October–December 2020, and January–June 2021). We examine mortality and utilization rates using logistic regressions, adjusting for demographic characteristics, prior chronic conditions, and geographic location (including residence near a reservation). We stratify the analysis by rural vs. nonrural counties.Data Sources and Analytic SampleWe use the New York State Medicaid claims data for the analysis.Principal FindingsWe find that even among Medicaid beneficiaries, who are similar in socioeconomic status and identical in health insurance coverage, AIAN populations have much lower rates of use of outpatient services and much higher rates of acute (inpatient and emergency room) service utilization for both ILI and COVID‐19 than non‐Hispanic Whites. Prior to COVID‐19, demographic and health status‐adjusted all‐cause mortality rates, including from ILI, were lower among American Indians than among non‐Hispanic Whites on New York State Medicaid, but this pattern reversed during the COVID‐19 pandemic. Both findings are driven by nonrural counties. We did not observe significant differences in all‐cause mortality and acute service utilization comparing AIAN to non‐Hispanic Whites in rural areas.ConclusionThe utilization and mortality disparities we identify within the Medicaid population highlight the need to move beyond insurance in addressing poor health outcomes in the American Indian population.
The application of GIS technology in building a multivariate taphonomic profile for improving PMI estimations in Greece
Authors: Karydi Christina , Montesantos Ioannis , Moraitis Konstantinos 
Published: October 2024

AbstractEnvironmental conditions highly affect decomposition rates and therefore a forensic practitioner should consider context‐specific information when estimating the post mortem interval (PMI). Traditional methods of collecting environmental data, however, are time‐consuming and often impractical for large‐scale studies or routine forensic investigations. This study developed an automated computer method by employing the technology of geographic information systems (GIS) and Python programming language to provide contextual information for bodies found outdoors in Greece. The generated coding script underwent testing on 95 bodies in various stages of decomposition, which were examined between the years 1999 and 2022 at the National and Kapodistrian University of Athens and the Forensic Medical Service of Thessaloniki. Using ArcGIS Pro software and publicly available online data, a multilayer map was developed. Individual layers included high‐resolution aerial images and data on the European Nature Information System ecosystem type, the Köppen–Geiger climatic type, the population density, the elevation, and the slope. Additionally, 99 national weather stations and their corresponding meteorological data were integrated. By leveraging the geographical coordinates of the recovery site of each case and information about the decedent's disappearance and recovery dates, this script automatically generates details from each of the above layers. Additionally, it calculates the accumulated degree days (ADD) and accumulated humidity days (AHD) values by extracting data from the nearest weather station. The GIS‐based approach enables rapid, objective, and reproducible taphonomic profile construction, which can greatly improve the reliability of PMI estimations. By utilizing this method, forensic practitioners can accurately evaluate environmental effects on decomposition, thus standardizing taphonomic profiling globally.
Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1
Authors: Rey Redondo Elvira , Leung Shara Ka Kiu , Yung Charmaine Cheuk Man 
Published: October 2024

AbstractMamiellophyceae are a ubiquitous class of unicellular green algae in the global ocean. Their ecological importance is highlighted in studies focused on the prominent genera Micromonas, Ostreococcus, and Bathycoccus. Mamiellophyceae are susceptible to prasinoviruses, double‐stranded DNA viruses belonging to the nucleocytoplasmic large DNA virus group. Our study represents the first isolation of a prasinovirus in the South China Sea and the only one to infect the globally distributed genus Mantoniella. We conducted a comparative analysis with previously identified viral relatives, encompassing morphological characteristics, host specificity, marker‐based phylogenetic placement, and whole‐genome sequence comparisons. Although it shares morphological and genetic similarities with established prasinoviruses, this novel virus showed distinct genetic traits, confining its infection to the species Mantoniella tinhauana. We also explored the global biogeography of this prasinovirus and its host by mapping metagenomic data and analysing their relationship with various environmental parameters. Our results demonstrate a pronounced link between the virus and its host, both found predominantly in higher latitudes in the surface ocean. By gaining an increased understanding of the relationships between viruses, hosts, and environments, researchers can better make predictions and potentially implement measures to mitigate the consequences of climate change on oceanic processes.
Unveiling the hub genes associated with ochratoxin A‐induced hepatotoxicity in broiler chickens
Authors: Li Xiaofeng , Wang Zhongyuan , Wang Shujuan , Yang Bing 
Published: October 2024

AbstractOchratoxin A (OTA) widely exists in raw food and feed materials and can induce liver damage and toxicity. However, the mechanisms of OTA‐induced hepatotoxicity were largely unknown. Thus, our study aimed to uncover the vital genes relevant to OTA‐induced hepatotoxicity in broiler chickens. Gene expression data of chicken embryo primary hepatocytes (CEPHs) in OTA‐treated and control groups were obtained from the GEO database. Totally 1407 differentially expressed genes (DEGs) were selected, of which 850 and 557 genes were up‐ and downregulated in OTA‐treated CEPHs. Gene ontology (GO) enrichment revealed that the DEGs were in connection with various biological processes, such as signal transduction, extracellular matrix organization, axon guidance, cell division, cholesterol homeostasis, proteolysis, microtubule cytoskeleton organization, and chromosome segregation. Pathway enrichment showed that the DEGs were related to metabolic pathways, ferroptosis, calcium, FoxO, Wnt, cell cycle, apoptosis, calcium, and cell adhesion molecules signaling pathways. Furthermore, the hub genes, including CDK1, DLGAP5, KIF2C, VCL, ITGB3, and ZYX, were identified as hub genes potentially contributing to OTA‐induced hepatotoxicity. Taken together, this study provides valuable insights into the mechanisms underlying OTA‐induced hepatotoxicity in broiler chickens.
N‐terminal targeting sequences and coding sequences act in concert to determine the localization and trafficking pathway of apicoplast proteins in Toxoplasma gondii
Authors: Anjum Sofia , Prasad Aparna , Mastud Pragati , Mishra Geetanjali , Patankar Swati 
Published: October 2024

AbstractBackgound InformationToxoplasma gondii has a relict plastid, the apicoplast, to which nuclear‐encoded proteins are targeted after synthesis in the cytosol. Proteins exclusively found in the apicoplast use a Golgi‐independent route for trafficking, while dually targeted proteins found in both the apicoplast and the mitochondrion use a Golgi‐dependent route. For apicoplast targeting, N‐terminal signal sequences have been shown to direct the localization of different reporters. In this study, we use chimeric proteins to dissect out the roles of N‐terminal sequences and coding sequences in apicoplast localization and the choice of the trafficking route.ResultsWe show that when the N‐termini of a dually targeted protein, TgTPx1/2, or of an apicoplast protein, TgACP, are fused with the reporter protein, enhanced green fluorescent protein (eGFP) or endogenous proteins, TgSOD2, TgSOD3, TgACP, or TgTPx1/2, the chimeric proteins exhibit flexibility in apicoplast targeting depending on the coding sequences. Further, the chimeras that are localized to the apicoplast use different trafficking pathways depending on the combination of the N‐terminal signals and the coding sequences.Conclusion and SignificanceThis report shows, for the first time, that in addition to the N‐terminal signal sequences, targeting and trafficking signals also reside within the coding sequences of apicoplast proteins.
Trichophyton erinacei‐Infektion beim Menschen: Fallbericht und Literaturübersicht
Authors: Cammarata Edoardo , Cristo Nunzia Di , Airoldi Chiara , Veronese Federica , Esposto Elia , Giacomazzi Claudio , Coppo Paola , Zavattaro Elisa , Savoia Paola 
Published: October 2024

ZusammenfassungHintergrundTrichophyton (T.) erinacei ist ein seltener, aber zunehmender zoonotischer Dermatophyt, der nur vereinzelt als Krankheitserreger beim Menschen isoliert wird, und nur wenige Fälle sind in der Literatur ausführlich beschrieben worden.Patienten und MethodenWir führten eine systematische Suche nach passenden Artikeln über demografische und klinische Merkmale sowie über Behandlungsansätze bei humanen T. erinacei‐Infektionen.ErgebnisseIn der internationalen Literatur wurde zwischen dem Beginn der Suche und November 2023 über 168 Patienten mit T. erinacei berichtet, wovon nur 56 Fälle (33,3%) vollständig sind. Das mittlere Alter bei der Diagnose betrug 26 Jahre, das Verhältnis zwischen Frauen und Männern lag bei etwa 2 zu 1. Die Hauptquelle der Erkrankung war der Igel. Die Infektion präsentierte sich mit einer Kombination aus Erythemen, schuppenden Plaques, Pusteln, Papeln, Bläschen, Ödemen und Erosionen; die häufigsten Lokalisationen waren die Hände und der Kopf. Die am häufigsten durchgeführte Untersuchung war die Pilzkultur, allerdings haben die Gensequenzierung und die Massenspektrometrie sowohl die Geschwindigkeit als auch die Präzision im jüngsten Diagnoseverlauf verbessert. Topisches Clotrimazol und systemisches Terbinafin waren die am öftesten gewählten Behandlungen.SchlussfolgerungenTrichophyton erinacei sollte bei Patienten mit erythematösen, schuppigen Flecken und kürzlich erfolgtem Kontakt mit Igeln in Betracht gezogen werden. Terbinafin sollte als wirksame Erstlinienbehandlung angesehen werden, Griseofulvin und Azole könnten als valide Alternativen gelten.
Häufigkeit und diagnostische Befunde von Typ‐I‐ und Typ‐IV‐Reaktionen gegen Beta‐Laktam‐Antibiotika – Bedeutung des Zeitintervalls zwischen klinischer Reaktion und Hauttestung
Authors: Farmer Kimberley , Oestmann Elsbeth , Worm Margitta 
Published: October 2024

ZusammenfassungHintergrundDie Prävalenz von Allergien gegen Beta‐Laktam‐Antibiotika wird aufgrund anamnestischer Daten deutlich überschätzt – mit bedeutenden medizinischen und wirtschaftlichen Folgen. Ziel der Arbeit war, die Häufigkeit von Sofort‐ und Spättypreaktionen gegenüber Beta‐Laktam‐Antibiotika zu bestimmen, um Diagnostik und Therapie zu optimieren. Ein Schwerpunkt lag auf dem Zeitintervall zwischen Reaktion und Testung.Patienten und MethodikAnamnestische und diagnostische Daten von Patienten mit Verdacht auf Allergie gegen Beta‐Laktam‐Antibiotika, die zwischen 2020 und 2022 in unserer Abteilung untersucht wurden, wurden ausgewertet.Ergebnisse27/116 (23%) Patienten reagierten in den Hauttestungen. Typ‐I‐Sensibilisierungen wurden bei 4/35 Patienten (11%) nachgewiesen, Typ‐IV‐Sensibilisierungen bei 23/83 (28%). Bei negativer In‐vitro‐Diagnostik und Hauttestung erfolgten stationäre Provokationstestungen bei 41/89 (46%). Typ‐I‐Allergien wurden bei zwei der 13 provozierten Patienten (15%) mit Soforttypreaktion, Typ‐IV‐Allergien bei drei von 29 (10%) mit Spättypreaktion bestätigt. Die Ergebnisse hingen deutlich mit dem Zeitintervall nach Reaktion zusammen. Bei einem Zeitintervall unter einem Jahr reagierten 19% (22/116), hingegen nur 4% (5/116) bei einem Zeitintervall über einem Jahr (bei Typ‐I‐Reaktion 9% vs. 3%; bei Typ‐IV‐Reaktion 23% vs. 5%.)SchlussfolgerungenDie Bedeutung des Zeitintervalls zwischen klinischem Ereignis und allergologischer Testung unterstützt die Leitlinienempfehlung zur Hauttestung innerhalb eines Jahres. Leitlinienempfehlungen zum diagnostischen Vorgehen bei länger zurückliegenden unklaren Reaktionen sind wünschenswert, um Allergien gegen Beta‐Laktam‐Antibiotika auszuschließen.
Pathogenetische Bedeutung von Antikörpern gegen Desmoglein 3 bei Patienten mit oralem Lichen planus
Authors: Didona Dario , Schmidt Morna F. , Meier Katharina , Mesas‐Fernandez Alberto , Maglie Roberto , Antiga Emiliano , Klemp Marisa , Yazdi Amir S. , Ghoreschi Kamran , Hertl Michael , Möbs Christian , Solimani Farzan 
Published: October 2024

ZusammenfassungHintergrund und ZieleDer orale Lichen planus (OLP) ist eine T‐Zell‐gesteuerte Erkrankung, welche die Lebensqualität der Betroffenen erheblich beeinträchtigt. Es konnte gezeigt werden, dass sowohl zelluläre als auch humorale gegen Desmoglein (Dsg) 1 und 3 gerichtete Mechanismen an der Pathogenese des OLP beteiligt sein könnten. Hier sollen die Häufigkeit des Auftretens und die pathologische Bedeutung von Anti‐Dsg‐Antikörpern anhand einer großen Kohorte von OLP‐Patienten analysiert werden.Material und MethodikMittels eines ELISA (enzyme‐linked immunosorbent assay) wurden Seren von OLP‐Patienten aus drei tertiären Referenzzentren auf Anti‐Dsg‐Antikörper untersucht. OLP‐Seren, die Anti‐Dsg‐Antikörper aufwiesen, wurden mittels Western Blot und Dispase‐basiertem Keratinozyten‐Dissoziations‐Assay (DDA) analysiert, um eine mögliche Bindungsstelle an der Dsg‐Ektodomäne zu identifizieren und ihre Pathogenität zu bewerten.ErgebnisseEs wurden Seren von 151 Patienten mit OLP untersucht, wobei vier Patienten (2,6%) mit erosivem Subtyp Antikörper gegen Dsg1 und/oder Dsg3 aufwiesen. Die Western‐Blot‐Analyse mit rekombinanten Dsg3‐Ektodomänen ergab hauptsächlich die Erkennung der Ektodomäne 5. Keines der vier Patientenseren führte im DDA mit spontan immortalisierten menschlichen Keratinozyten zur Akantholyse.SchlussfolgerungenDie Aktivierung der humoralen Immunität tritt vor allem bei Patienten mit erosivem OLP auf, wahrscheinlich aufgrund eines epitope spreadings. Da die OLP‐Serumantikörper in vitro keinen Effekt auf die interzelluläre Adhäsion haben, ist davon auszugehen, dass sie nicht ursächlich für die Krankheit sind, sondern eher eine Nebenerscheinung darstellen.
S3‐Leitlinie: Diagnostik und Therapie der epidermalen Nekrolyse (Stevens‐Johnson‐Syndrom und toxisch epidermale Nekrolyse) – Teil 1: Diagnostik, initiales Management und immunmodulierende Systemtherapie
Authors: Heuer Ruben , Paulmann Maren , Annecke Thorsten , Behr Björn , Boch Katharina , Boos Anja M. , Brockow Knut , French Lars E. , Gille Jochen , Gundlach Verena , Hartmann Bernd , Höger Peter , Hofmann Silke C. , Klein Tobias , Lehnhardt Marcus , Liß Yvonne , Maier Philip , Mandel Philipp , Marathovouniotis Nicos , Marlok Finnja , Mittelviefhaus Hans , Pleyer Uwe , Pradeau Marie , Rall Katharina , Rieg Siegbert , Rittner Heike , Sander Frank , Schnitzler Stefan , Schut Christina , Stolle Annette , Vorobyev Artem , Wedi Bettina , Weiss Johannes , Zepp Maximilian , Ziemer Mirjana , Mockenhaupt Maja , Nast Alexander 
Published: October 2024

ZusammenfassungDas Stevens‐Johnson‐Syndrom und die toxisch epidermale Nekrolyse (SJS/TEN) sind seltene, in erster Linie arzneimittelinduzierte, akut lebensbedrohliche Erkrankungen der Haut und Schleimhäute. Sie werden heute als Varianten einer Krankheitsentität mit unterschiedlichem Schweregrad betrachtet, die als epidermale Nekrolyse (EN) bezeichnet wird. Die epidermale Nekrolyse geht mit hoher Morbidität und Letalität sowie großer Krankheitslast für die Betroffenen einher. Die Leitlinie „Diagnostik und Therapie der epidermalen Nekrolyse (Stevens‐Johnson‐Syndrom und toxisch epidermale Nekrolyse)“ wurde unter systematischer Berücksichtigung bestehender wissenschaftlicher Literatur und im formalen Konsensusverfahren nach dem Regelwerk der AWMF entwickelt, um klinische Entscheidungsfindungen evidenzbasiert zu unterstützen. Die interdisziplinäre Leitlinienkommission bestand aus Vertretern verschiedener Fachgesellschaften sowie aus Patientenvertretern. Die Leitlinie richtet sich an Ärzte der Fachgebiete Dermatologie, Ophthalmologie, plastische Chirurgie, Intensivmedizin sowie Kinder‐ und Jugendmedizin in Klinik und Praxis sowie an andere medizinische Fachgebiete, die an der Diagnostik und Behandlung von EN beteiligt sind. Zusätzlich richtet sich die Leitlinie an Patienten, deren Angehörige, Kostenträger und politische Entscheidungsträger. Dieser erste Teil behandelt die Themen Diagnostik, initiales Management sowie immunmodulierende Systemtherapie.
Real‐World Evidence on Prognostic Value of MRD in Multiple Myeloma Using Flow Cytometry
Authors: Muronova Ludmila , Soucek Ondrej , Zihala David , Sevcikova Tereza , Popkova Tereza , Plonkova Hana , Venglar Ondrej , Pour Ludek , Stork Martin , Rihova Lucie , Bezdekova Renata , Minarik Jiri , Látal Vojtech , Novak Martin , Jungova Alexandra , Dekojova Tereza , Straub Jan , Spacek Martin , Rezacova Vladimira , Maisnar Vladimir , Radocha Jakub , Hajek Roman , Jelinek Tomas 
Published: October 2024

ABSTRACTMinimal residual disease (MRD) is one of the most important prognostic factors in multiple myeloma (MM) and a valid surrogate for progression‐free survival (PFS) and overall survival (OS). Recently, MRD negativity was approved as an early clinical endpoint for accelerated drug approval in MM. Nevertheless, there is limited evidence of MRD utility in real‐world setting. In this retrospective multicenter study, we report outcomes of 331 newly diagnosed MM patients with MRD evaluation at Day+100 after autologous stem cell transplantation using flow cytometry with a median limit of detection of 0.001%. MRD negativity was reached in 47% of patients and was associated with significantly prolonged median PFS (49.2 months vs. 18.4 months; hazard ratios (HR) = 0.37; p < 0.001) and OS (not reached vs. 74.9 months; HR = 0.50; p = 0.007). Achieving MRD negativity was associated with PFS improvements regardless of age, International Staging System (ISS) stage, lactate dedydrogenase (LDH) level, or cytogenetic risk. Importantly, MRD positive patients benefited from lenalidomide maintenance versus no maintenance (18‐months PFS: 81% vs. 46%; HR = 0.24; p = 0.002) while in MRD negative patients such benefit was not observed (p = 0.747). The outcomes of our real‐world study recapitulate results from clinical trials including meta‐analyses and support the idea that MRD positive patients profit more from lenalidomide maintenance than MRD negative ones.
Corrosion resistance of insulating refractories for the synthesis of lithium‐ion battery LiCoO2 cathode materials
Authors: Yang Biao , Yin Bo , Chen Han , Zheng Yifeng 
Published: October 2024

AbstractLiCoO2 has become the most widely used cathode material in lithium‐ion batteries because of its high capacity and excellent stability. The high‐temperature solid‐state method is commonly used for the preparation of LiCoO2. However, this method will produce highly penetrating Li2O, which causes spall or fracture of the insulating refractory materials in the kiln. In this study, the corrosion resistance of bubble alumina, mullite, and calcium hexaaluminate (CA6) insulating refractories to LiCoO2 has been thoroughly investigated. Combining the laboratory‐scale interfacial reaction experiments with post‐experimental life cycle analysis of industrial insulating refractories, the interaction between the insulating refractory materials and LiCoO2 after calcination at 900°C for 5 h and the corrosion behavior of LiCoO2 on different insulating refractory materials following heat treatment at 900°C for 5 h every time and repeated seven times are investigated. The corrosion mechanisms are concluded by analyzing the physicochemical composition and macro‐ and micromorphology of the three insulating refractory materials before and after corrosion. The results can provide a basis for the use of insulating refractories in the development of lithium batteries.