bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glucocorticoid receptor response and glucocorticoid-induced leucine zipper expression in neutrophils of critically ill patients with traumatic and non-traumatic brain injury
Authors: Lotsios N.S. , Vrettou C.S. , Poupouzas G. , Chalioti A. , Keskinidou C. , Pratikaki M. , Giannopoulou V. , Kotanidou A. , Vassiliadi D.A. , Dimopoulou Ioanna , Vassiliou A.G. 
Published: September 2024

ObjectiveCritically ill patients, including those with brain injuries (BI), are frequently hospitalized in an intensive care unit (ICU). As with other critical states, an adequate stress response is essential for survival. Research on the hypothalamic-pituitary-adrenal gland (HPA) axis function in BI has primarily focused on assessing ACTH and cortisol levels. However, the immunological, metabolic, and hemodynamic effects of glucocorticoids (GCs) are mediated through the glucocorticoid receptor (GCR), a ubiquitously distributed intracellular receptor protein. Data on GCR-α expression and its signaling in acute BI injury are lacking.MethodsWe designed a prospective observational study, carried out in one academic multi-disciplinary ICU. Forty-two critically ill patients with acute (BI)were included. These patients suffered from traumatic BI (N= 20), subarachnoid hemorrhage (N= 12), intracranial hemorrhage (N= 7), or ischemic stroke (N= 3). All patients were steroid-free. Twenty-four age and sex-matched healthy controls were used for comparison.ResultsExpression of GCR-α and the glucocorticoid-inducible leucine zipper (GILZ), serum cortisol, interleukins (IL) 6, 8, 10 and TNF- α, and the BI biomarkers glial fibrillary acidic protein (GFAP) and total Tau were measured on ICU admission (within 48 hours) and 5-7 days from admission. Compared to healthy controls, in the critically ill patients with BI, GCR-α mRNA expression was significantly downregulated on admission, and after 5-7 days in the ICU (2.3-fold, p&lt;0.05 and 2.6-fold, p&lt;0.01, respectively). Even though GCR-α was downregulated, its downstream gene, GILZ, was expressed at the same levels as in normal controls on admission and was significantly upregulated 5-7 days following admission (2-fold, p&lt;0.001). TNF-α levels were undetectable at both time-points. GCR-α expression levels inversely correlated with IL-6. The levels of cortisol and the BI biomarkers did not differ between the 2 time-points.ConclusionsWe provide novel evidence on the downregulated expression and upregulated signaling of the ligand-binding and functionally active GCR-α isoform in the polymorphonuclear neutrophils (PMNs) of critically ill patients with BI. The increased GILZ expression indicates an increased GC sensitivity in the PMNs of BI critically ill patients.
Combined toxicity of multiwall carbon nanotubes and cadmium on rice (Oryza sativa L.) growth in soil
Authors: Long Jinghua , Wang Xuanxuan , Zhang Wei 
Published: September 2024

The comprehensive effects of nanoparticles and coexisting heavy metals on plant growth are still unclear, especially in soil medium. The single and combined effects of multiwall carbon nanotubes (MWCNTs) and cadmium (Cd) on rice (Oryza sativa L.) growth were examined in this study through a 4 months pot experiment in 2022. Rice plants were exposed to different concentrations of MWCNTs (100 and 500 mg kg−1) in the presence of 5.0 mg kg−1 Cd stress. At the tillering stage, the 500 mg kg−1 MWCNTs addition reduced plant height by 8.0% and increased soluble protein content in the leaves by 13.7%, demonstrating that a single MWCNTs had a slight negative impact on rice growth. When exposed to Cd stress, the inclusion of 500 mg kg−1 MWCNTs led to a 6.7%–9.0% decrease in bioavailable Cd level in soil, resulting in considerable reductions in Cd content in roots (23.4%–29.9%), shoots (24.5%–28.3%) and grains (28.3%–66.2%). Compared to the single Cd treatment, the O. sativa L. leaves treated with Cd and MWCNTs (500 mg kg−1) had considerably reduced levels of malondialdehyde (MDA), soluble protein, and activities of antioxidant enzymes (POD, CAT, and SOD). The findings of this study indicated that appropriate concentrations of MWCNTs application in soil could alleviate Cd-induced toxicity on rice growth.
Comparative efficacy of different single drugs to prevent necrotizing enterocolitis in preterm infants: an update systematic review and network meta-analysis
Authors: Chen Jing , Chen Xiao , Huang Xiaoling , Liu Jia , Yu Qingfeng 
Published: September 2024

ObjectiveTo investigate an optimal regimen of six drugs, including lactoferrin, probiotics, prebiotics, glutamine, arginine and erythropoietin (EPO), for the prevention of necrotizing enterocolitis (NEC) in preterm infants.MethodsPubMed, Embase, Ovid, The Cochrane Library, and Web of Science databases were searched for randomized controlled trials (RCTs) investigating the efficacy of lactoferrin, probiotics, prebiotics, glutamine, arginine, and EPO in preventing NEC in preterm infants, with a cutoff date of June 20, 2024. Two authors independently screened studies and extracted all the data. Network meta-analysis (NMA) was conducted to compare the outcomes of different interventions, and group rankings were determined using the surface under the cumulative ranking curve (SUCRA).ResultsA total of 89 RCTs with 26,861 preterm infants were included. Arginine demonstrated the highest clinical efficacy in reducing the incidence of NEC, with probiotics being the next most effective and the placebo being the least effective. Lactoferrin was identified as the most effective intervention for reducing the incidence of NEC-associated sepsis. Prebiotics showed the highest effect on overall mortality, reducing the beginning of enteral feeding, and were associated with the shortest hospital stay. Glutamine significantly decreased the time to full enteral feeding.ConclusionExisting literature highlights arginine as the most efficacious pharmacological agent in preventing NEC in preterm infants. It has been shown to effectively lower the rates of NEC, septicemia, and mortality, warranting its recommendation as the first-line clinical intervention. Following this, probiotics are recommended as a second option.
Overexpression of serum HMGB1 and IDO in esophageal squamous cell carcinoma patients: potential clinical auxiliary diagnostic markers and immunotherapeutic targets
Authors: Cui Wenxuan , Niu Yinghao , Zhang Xueyuan , Huang Beixuan , Shang Xiaoya , Zhao Wei , Yan Xi , Mi Yunqiang , Ma Ming , Zhang Jinyan , Yang Xingxiao 
Published: September 2024

BackgroundHigh mobility group box 1 (HMGB1) and indoleamino-2, 3-dioxygenase (IDO) participate in the occurrence and development of esophageal squamous cell carcinoma (ESCC), regulate the tumor immune microenvironment, and are closely related to tumor growth and metastasis. However, the regulatory mechanism of serum HMGB1 and IDO has not been clarified and needs further exploration.MethodsBlood samples of 55 ESCC patients initially hospitalized in the Fourth Hospital of Hebei Medical University from August 2021 to January 2022 were selected as the ESCC group, and relevant clinical data were collected, and blood samples from 40 healthy people during the same period were selected as the control group. Serum HMGB1 and IDO levels were determined by ELISA, and lymphocyte subsets in peripheral blood of all subjects were detected by flow cytometry. The correlation between the expression levels of HMGB1 and IDO in ESCC cells was detected by Western blot.ResultsSerum HMGB1 and IDO levels were significantly increased in ESCC patients, and with the progression of ESCC patients, serum HMGB1 and IDO levels were also gradually increased; serum HMGB1 was significantly correlated with IDO; serum HMGB1 and IDO combined with CEA and SCC-Ag were of high value in predicting the clinical progression of ESCC patients; the absolute counts of CD4+CD28+T cells and CD8+CD28+T cells in high HMGB1 group were significantly lower than those in low HMGB1 group, while the percentage of CD4+PD-1+T cells was significantly higher than that in low HMGB1 group; the percentage and absolute counts of CD4+CD28+T cells and the absolute counts of CD8+CD28+T cells in high IDO group were significantly lower than those in the low IDO group, while the percentage of CD8+PD-1+T cells was significantly higher than that in the low IDO group; increased serum HMGB1 and IDO expression levels were closely related to poor prognosis in ESCC patients; and HMGB1 may promote IDO expression by activating NF-κB signaling pathway.ConclusionSerum HMGB1 and IDO have a synergistic effect, they inhibit immune function and promote tumor progression in ESCC patients, and also lead to poor prognosis.
An investigation into the correlation between intraperitoneal teicoplanin concentrations and treatment outcomes in peritoneal dialysis-associated peritonitis
Authors: Wang Lulu , Fan Jiangqing , Chen Xuejie , Lei Wenpu , Jiang Chunming , Liu Hang , Yang Yun , Shen Jizhong 
Published: September 2024

Peritoneal dialysis-associated peritonitis (PDAP) is a frequent complication of peritoneal dialysis. The guidelines from the International Society for Peritoneal Dialysis (ISPD) suggest administering teicoplanin through the peritoneal route to treat PDAP, but do not specify the ideal concentration for peritoneal dialysis effluent (PDE). Patients meeting the trial criteria for PDAP in our hospital between July 2022 and December 2023 were enrolled. Data on PDE white blood cell count, PDE neutrophil percentage, clinical symptoms, CRP, and PCT were gathered pre- and post-treatment. Incidences of adverse drug reaction (ADR) and case numbers during treatment were recorded. Subsequently, patients were categorized into cured and uncured groups for evaluating the relationship between PDE teicoplanin concentration and treatment effectiveness. The self-control study results on teicoplanin efficacy indicated intraperitoneal teicoplanin administration achieved an efficacy rate of 88.9% and an ADR incidence of 5.5% in treating PDAP patients. There was no observed correlation between teicoplanin blood concentration and PDE concentration. PDE teicoplanin concentrations on days 1, 3, 5, and 7 post-dosing were higher inthe cured group, with a significant contrast in PDE concentration on day 5 between the 18.98 ± 2.43 mg/L of the cured group and the 12.07 ± 2.68 mg/L of the uncured group. ROC curve revealed a higher likelihood of cure in patients when PDE teicoplanin concentration exceeded 15.138 mg/L on day 5 post-dosing. Univariate and multifactorial studies identified 24-h urine volume and the number of daily abdominal dialysis sessions as influential factors in PDE teicoplanin concentration on day 5. A positive correlation was found between 24-h urine volume and PDE teicoplanin concentration, with PDAP patients having urine volume over 537 mL showing significantly higher drug concentrations. Conversely, the number of daily PDAP sessions was negatively correlated with PDE teicoplanin concentrations, indicating that patients with 1∼3 daily PDAP sessions had notably higher PDE teicoplanin concentrations compared to those with 4∼6 sessions. Therefore, PDAP patients who use intraperitoneal teicoplanin could effectively control infection by monitoring the PDE teicoplanin concentration (&gt;15.138 mg/L) on day 5 after dosing.
The feeding preference and bite response between Microtus fortis and Broussonetia papyrifera
Authors: Wang Shuangye , Chen Zihao , Wang Mengxin , Zhang Meiwen , Zhang Chen , Huang Tian , Zhao Yunlin , Xu Zhenggang 
Published: September 2024

IntroductionBroussonetia papyrifera is a dioecious plant that is rich in various metabolites and widely distribute in Asia. Microtus fortis is a rodent that often causes damage to crops, especially in the Dongting Lake region of China. There is a wide overlap in the distribution areas for the above species and the M. fortis feeds on the leaves of the B. papyrifera. Preliminary experiments have shown that the reproduction of M. fortis is inhibited after feeding on the leaves of the B. papyrifera.MethodsIn order to explore the potential of using B. papyrifera to develop botanical pesticides, we investigated the palatability and reactive substances. The feeding frequency of M. fortis on B. papyrifera leaves to that of on daily fodder and Carex brevicuspis that is the primary food for the wild population were compared. We also attempted to identify the responsive substances in B. papyrifera leaves that were bitten by M. fortis using metabolome analysis.ResultsIn general, B. papyrifera leaves exhibited a stronger attraction to M. fortis. M. fortis foraged B. papyrifera leaves more frequently, and the intake was higher than that of the other two. Differential metabolites were screened by comparing normal leaves and leaves bitten by M. fortis, meanwhile with the intervention of clipped leaves. A total of 269 substances were screened, and many of these were involved in the biosynthesis of secondary metabolites, including terpenoids and alkaloids. These substances may be related to the defense mechanism of B. papyrifera against herbivores.DiscussionThese findings support further research examining animal–plant interactions and simultaneously provide insights into the utilisation of B. papyrifera resources and the management of rodents. The good palatability and the defense of B. papyrifera leaves suggest that they have the potential to contribute in development of plant rodenticide.
Bacterial infections and outcomes of inpatients with COVID-19 in the intensive care unit during the delta-dominant phase: the worst wave of pandemic in Iran
Authors: Akbari Mojtaba , Dehghani Yeganeh , Shirzadi Mohammad , Pourajam Samaneh , Hosseinzadeh Majid , Sajadi Mahdi , Alenaseri Malihe , Siavash Mansour , Jafari Leila , Solgi Hamid 
Published: September 2024

BackgroundEpidemiological data regarding the prevalence of bacterial multidrug-resistant (MDR) Gram-negative infections in patients with COVID-19 in Iran are still ambiguous. Thus, in this study we have investigated the epidemiology, risk factors for death, and clinical outcomes of bacterial infections among patients with COVID-19 in the intensive care unit (ICU).MethodThis retrospective cohort study included patients with COVID-19 hospitalized in the ICU of a university hospital in Iran between June 2021 and December 2021. We evaluated the epidemiological, clinical, and microbiological features, outcomes and risk factors associated with death among all COVID-19 patients. Data and outcomes of these patients with or without bacterial infections were compared. Kaplan–Meier plot was used for survival analyses.ResultsIn total, 505 COVID-19 patients were included. The mean age of the patients was 52.7 ± 17.6 years and 289 (57.2%) were female. The prevalence of bacterial infections among hospitalized patients was 14.9%, most of them being hospital-acquired superinfections (13.3%). MDR Klebsiella pneumoniae and Staphylococcus aureus were the most common pathogens causing respiratory infections. Urinary tract infections were most frequently caused by MDR Escherichia coli and K. pneumoniae. The overall in-hospital mortality rate of COVID-19 patients was 46.9% (237/505), while 78.7% (59/75) of patients with bacterial infections died. Infection was significantly associated with death (OR 6.01, 95% CI = 3.03–11.92, p-value &lt;0.0001) and a longer hospital stay (p &lt; 0.0001). Multivariate logistic regression analysis showed that Age (OR = 1.04, 95% CI = 1.03–1.06, p-value &lt;0.0001), Sex male (OR = 1.70, 95% CI = 1.08–2.70, p-value &lt;0.0001), Spo2 (OR = 1.99, 95% CI = 1.18–3.38, p-value = 0.010) and Ferritin (OR = 2.33, 95% CI = 1.37–3.97, p-value = 0.002) were independent risk factors associated with in-hospital mortality. Furthermore, 95.3% (221/232) of patients who were intubated died.ConclusionOur findings demonstrate that bacterial infection due to MDR Gram-negative bacteria associated with COVID-19 has an expressive impact on increasing the case mortality rate, reinforcing the importance of the need for surveillance and strict infection control rules to limit the expansion of almost untreatable microorganisms.
Trajectories of cognitive function development and predictive factors in disabled middle-aged and older adults
Authors: Pang Jiaxue , Xu Yang , Liu Qiankun , Huang Juju , Li Pengyao , Ma Li , Zeng Chunlu , Ma Xiaoqing , Xie Hui 
Published: September 2024

ObjectiveTo explore the trajectories of cognitive function development and predictive factors in disabled middle-aged and older adults.MethodsUtilizing data from 983 disabled middle-aged and older adults in the China Health and Retirement Longitudinal Study (CHARLS) from 2013 to 2020, latent growth mixture models were constructed to analyze the categories of cognitive function development trajectories and their predictive factors.ResultsThe cognitive function trajectories of the disabled middle-aged and older adults were classified into three categories: rapid decline (32.6%), Slow decline (36.1%), and Stable (31.2%). Multinomial logistic regression analysis identified age, gender, residence, education, marital status, household income, sleep duration, depression, hearing ability, and social participation as predictors of these trajectories.ConclusionThere is heterogeneity in the cognitive function development trajectories among disabled middle-aged and older adults. Healthcare professionals can implement targeted health management based on the characteristics of different groups to prevent the deterioration of cognitive function in this population.
Malignancies After Heart Transplantation
Authors: Stenman Caroline , Wallinder Andreas , Holmberg Erik , Karason Kristjan , Magnusson Jesper , Dellgren Göran 
Published: September 2024

Heart transplant patients have an increased risk of developing cancer. Patients who underwent HTx between 1985 and 2017 were included. Detection of cancer was obtained by cross-checking the study population with the Swedish Cancer-Registry and the Cause-of-Death-Registry. A total of 664 patients were followed for a median of 7.7 years. In all, 231 malignancies were diagnosed in 138 patients. Compared to the general population the excess risk of cancer following HTx was 6.2-fold calculated as the standardized incidence ratio (SIR) and 2.9-fold after exclusion of non-melanoma skin cancer (NMSC). The most common malignancies were NMSC, non-Hodgins lymphoma, and lung cancer. There was no significant difference in overall survival between those with and without a history of cancer before HTx (p = 0.53). During a median follow-up of 7.7 years, 19% of HTx recipients developed cancer, 6.2-fold higher relative to the general population, and 2.9-fold higher when excluding NMSC. Risk factors for malignancies (excluding NMSC) included previous smoking, hypertension and prolonged ischemic time; and for NMSC, increasing age, seronegative CMV-donors, and azathioprine. A previous cancer in selected recipients results in similar survival compared to those without cancer prior to HTx.
Influence of Pain, Risk Factors, and Functional Ability on Physical Activity Levels in Women with Anterior Knee Pain: A Cross-Sectional Study
Authors: Alharbi Amjad Hajaj , Seyam Mohamed K. , Alanazi Ahmad , Almansour Ahmed , Hasan Shahnaz 
Published: September 2024

Background and Objectives: Anterior knee pain (AKP) refers to chronic prepatellar pain and is one of the most common knee complaints in physically active women. This condition can significantly affect daily activities and overall quality of life. This study aims to assess the impact of pain, risk factors, and functional ability on different levels of physical activity (comparing low versus moderate activity) in women with AKP. Materials and Methods: This cross-sectional study involved fifty-six women diagnosed with AKP (aged 20–45 years) who were equally allocated into low and moderate physical activity groups. Their AKP and functional ability were assessed using the visual analog scale (VAS) and double squats and step-down tests, respectively. Possible risk factors were assessed using the Q-angle, modified Thomas test, sit and reach test, and patellar glide test. A person’s correlation coefficient and independent t-tests were used to determine the relationship and the differences between these variables while keeping the confidence interval level at 95%. Result: Women with moderate activity levels showed significantly higher scores on the VAS than those with low activity levels (p = 0.040). However, both groups had no significant difference in their functional ability or potential risk factors (p > 0.05). Additionally, their functional ability (double squat) showed a positive association with hamstring flexibility (Pearson correlation coefficient [r]:0.3; p = 0.006). Conclusions: Women with AKP who were engaged in moderate physical activity experienced higher levels of pain compared to those with low activity levels. These findings underscore the urgent need for further investigation into different levels of physical activity to develop appropriate prevention and treatment strategies for women with AKP.
Impact of Land Use and Land Cover (LULC) Changes on Carbon Stocks and Economic Implications in Calabria Using Google Earth Engine (GEE)
Authors: Khachoo Yasir Hassan , Cutugno Matteo , Robustelli Umberto , Pugliano Giovanni 
Published: September 2024

Terrestrial ecosystems play a crucial role in global carbon cycling by sequestering carbon from the atmosphere and storing it primarily in living biomass and soil. Monitoring terrestrial carbon stocks is essential for understanding the impacts of changes in land use on carbon sequestration. This study investigates the potential of remote sensing techniques and the Google Earth Engine to map and monitor changes in the forests of Calabria (Italy) over the past two decades. Using satellite-sourced Corine land cover datasets and the InVEST model, changes in Land Use Land Cover (LULC), and carbon concentrations are analyzed, providing insights into the carbon dynamics of the region. Furthermore, cellular automata and Markov chain techniques are used to simulate the future spatial and temporal dynamics of LULC. The results reveal notable fluctuations in LULC; specifically, settlement and bare land have expanded at the expense of forested and grassland areas. These land use and land cover changes significantly declined the overall carbon stocks in Calabria between 2000 and 2024, resulting in notable economic impacts. The region experienced periods of both decline and growth in carbon concentration, with overall losses resulting in economic impacts up to EUR 357.57 million and carbon losses equivalent to 6,558,069.68 Mg of CO 2 emissions during periods of decline. Conversely, during periods of carbon gain, the economic benefit reached EUR 41.26 million, with sequestered carbon equivalent to 756,919.47 Mg of CO 2 emissions. This research aims to highlight the critical role of satellite data in enhancing our understanding and development of comprehensive strategies for managing carbon stocks in terrestrial ecosystems.