bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactions of North Pacific Tropical, Midlatitude, and Polar Disturbances Resulting in Linked Extreme Weather Events over North America in October 2007
Authors: Bosart Lance F. , Moore Benjamin J. , Cordeira Jason M. , Archambault Heather M. 
Published: December 2016

AbstractThis study uses observations and model reanalyses to examine the multiscale processes associated with four high-impact extreme weather events (EWEs) over North America during late October 2007. The EWEs consisted of wind-driven wildfires in California, prolonged anomalous cold conditions in Mexico linked to two cold surges, heavy rainfall in the eastern United States, and severe flood-producing heavy rainfall in southern Mexico. The EWEs involved a pronounced large-scale flow reconfiguration across the North Pacific and North America in conjunction with the formation of a high-amplitude Rossby wave train. The flow reconfiguration involved perturbations to the North Pacific jet stream linked to polar, midlatitude, and tropical disturbances, including three tropopause-level polar disturbances originating over northeastern Asia, transient extratropical cyclones, a diabatic Rossby vortex, and western North Pacific Tropical Cyclone Kajiki. Eulerian and Lagrangian diagnostics indicate that ridge amplification within the wave train was enhanced in connection with latent heat release along warm conveyor belts rooted in the tropics and subtropics over the North Pacific. Two anticyclonic Rossby wave breaking events over North America established synoptic-scale conditions that supported the EWEs. The results highlight how the large- and synoptic-scale flow can evolve to facilitate multiple geographically separated but dynamically linked EWEs. Based on the results, it is posited that during autumn the North Pacific jet stream may be particularly conducive to large-scale flow amplification, possibly resulting in EWEs, in response to perturbations associated with tropical, midlatitude, and polar disturbances.
Neutrophils and the S100A9 protein critically regulate granuloma formation
Authors: Yoshioka Yuya , Mizutani Tatsuaki , Mizuta Satoshi , Miyamoto Ayumi , Murata Satoru , Ano Toshiaki , Ichise Hiroshi , Morita Daisuke , Yamada Hiroyuki , Hoshino Yoshihiko , Tsuruyama Tatsuaki , Sugita Masahiko 
Published: December 2016

Key PointsS100A9+ neutrophils accumulated prominently in the central area of granulomas in humans and guinea pigs. Granuloma formation was markedly impaired by a treatment with the S100A9 inhibitor, tasquinimod.
COUSCOus: improved protein contact prediction using an empirical Bayes covariance estimator
Authors: Rawi Reda , Mall Raghvendra , Kunji Khalid , El Anbari Mohammed , Aupetit Michael , Ullah Ehsan , Bensmail Halima 
Published: December 2016

Abstract
Background
The post-genomic era with its wealth of sequences gave rise to a broad range of protein residue-residue contact detecting methods. Although various coevolution methods such as PSICOV, DCA and plmDCA provide correct contact predictions, they do not completely overlap. Hence, new approaches and improvements of existing methods are needed to motivate further development and progress in the field. We present a new contact detecting method, COUSCOus, by combining the best shrinkage approach, the empirical Bayes covariance estimator and GLasso.

Results
Using the original PSICOV benchmark dataset, COUSCOus achieves mean accuracies of 0.74, 0.62 and 0.55 for the top L/10 predicted long, medium and short range contacts, respectively. In addition, COUSCOus attains mean areas under the precision-recall curves of 0.25, 0.29 and 0.30 for long, medium and short contacts and outperforms PSICOV. We also observed that COUSCOus outperforms PSICOV w.r.t. Matthew’s correlation coefficient criterion on full list of residue contacts. Furthermore, COUSCOus achieves on average 10% more gain in prediction accuracy compared to PSICOV on an independent test set composed of CASP11 protein targets. Finally, we showed that when using a simple random forest meta-classifier, by combining contact detecting techniques and sequence derived features, PSICOV predictions should be replaced by the more accurate COUSCOus predictions.

Conclusion
We conclude that the consideration of superior covariance shrinkage approaches will boost several research fields that apply the GLasso procedure, amongst the presented one of residue-residue contact prediction as well as fields such as gene network reconstruction.
A digital enzyme-linked immunosorbent assay for ultrasensitive measurement of amyloid-β 1–42 peptide in human plasma with utility for studies of Alzheimer’s disease therapeutics
Authors: Song Linan , Lachno D. Richard , Hanlon David , Shepro Adam , Jeromin Andreas , Gemani Dipika , Talbot Jayne A. , Racke Margaret M. , Dage Jeffrey L. , Dean Robert A. 
Published: December 2016

Abstract
Background
Amyloid-β 1–42 peptide (Aβ1–42) is associated with plaque formation in the brain of patients with Alzheimer’s disease (AD). Pharmacodynamic studies of AD therapeutics that lower the concentrations of Aβ1–42 in peripheral blood require highly sensitive assays for its measurement. A digital enzyme-linked immunosorbent assay (ELISA) using single molecule array (Simoa) technology has been developed that provides improved sensitivity compared with conventional ELISA methods using the same antibody reagents.

Methods
A sensitive digital ELISA for measurement of Aβ1–42 using antibodies 3D6 and 21F12 was developed. Assay performance was evaluated by repeated testing of pooled human plasma and buffer diluent quality control samples to determine relative accuracy, intra- and inter-assay precision, limit of detection (LOD), lower limit of quantification (LLOQ), dilutional linearity, and spike recovery. The optimized assay was used to quantify Aβ1–42 in clinical samples from patients treated with the β-site amyloid precursor protein cleaving enzyme 1 inhibitor LY2886721.

Results
The prototype assay measured Aβ1–42 with an LOD of 0.3 pg/ml and an LLOQ of 2.8 pg/ml in plasma, calibrated using an Aβ1–42 peptide standard from Fujirebio. Assay precision was acceptable with intra- and inter-assay coefficients of variation both being ≤10%. Dilutional linearity was demonstrated in sample diluent and immunodepleted human plasma. Analyte spike recovery ranged from 51% to 93% with a mean of 80%. This assay was able to quantify Aβ1–42 in all of the 84 clinical samples tested. A rapid reduction in levels of Aβ1–42 was detected within 1 h after drug treatment, and a dose-dependent decrease of Aβ1–42 levels was also observed over the time course of sample collection.

Conclusions
This digital ELISA has potential utility in clinical applications for quantification of Aβ1–42 in plasma where high sensitivity and precision are required.
NK cells require antigen-specific memory CD4+ T cells to mediate superior effector functions during HSV-2 recall responses in vitro
Authors: Chen Branson , Lee Amanda J , Chew Marianne V , Ashkar Ali A 
Published: December 2016

Abstract
Natural killer (NK) cells have an important role in mounting protective innate responses against genital herpes simplex virus type 2 (HSV-2) infections. However their role as effectors in adaptive immune responses against HSV-2 is unclear. Here, we demonstrate that NK cells from C57BL/6 mice in an ex vivo splenocyte culture produce significantly more interferon γ (IFN-γ) upon re-exposure to HSV-2 antigens in a mouse model of genital HSV-2 immunization. We find that naïve NK cells do not require any prior stimulation or priming to be activated to produce IFN-γ. Our results demonstrate that HSV-2–experienced CD4+ T cells have a crucial role in coordinating NK cell activation and that their presence during HSV-2 antigen presentation is required to activate NK cells in this model of secondary immune response. We also examined the requirement of cell-to-cell contacts for both CD4+ T cells and NK cells. NK cells are dependent on direct interactions with other HSV-2–experienced splenocytes, and CD4+ T cells need to be in close proximity to NK cells to activate them. This study revealed that NK cells do not exhibit any memory toward HSV-2 antigens and, in fact, require specific interactions with HSV-2–experienced CD4+ T cells to produce IFN-γ
Low-frequency rotational isolator for airborne exploration
Authors: Sunderland Andrew , Lockwood Ray , Ju Li , Blair David G. 
Published: December 2016

We have determined the performance of a passive rotational vibration isolator for a time-domain airborne electromagnetic (TDEM) receiver. The isolator uses neutrally buoyant flotation to provide very soft suspension and a very low resonant frequency of 0.065 Hz ± 0.005 Hz. One of the limitations of mapping deeper targets in areas of conductive overburden with TDEM systems is that low-frequency coil-vibration noise provides a lower bound to the transmitter base frequency (typically limited to 25 Hz). The purpose of this new isolator is to improve coil vibration related noise between 5 and 20 Hz to allow the transmitter base frequency to be reduced. A fixed-wing flight test determined that a receiver inside the new isolator had five times less rotational noise at 10 Hz than a current commercial system.
Statistical analysis plan for the WOMAN-ETAPlaT study: Effect of tranexamic acid on platelet function and thrombin generation
Authors: Dallaku Kastriot , Shakur Haleema , Edwards Phil , Beaumont Danielle , Roberts Ian , Huque Sumaya , Delius Maria , Mansmann Ulrich 
Published: December 2016

Background. Postpartum haemorrhage (PPH) is a potentially life-threatening complication for women, and the leading cause of maternal mortality. Tranexamic acid (TXA) is an antifibrinolytic used worldwide to treat uterine haemorrhage and to reduce blood loss in general surgery. TXA may have effects on thrombin generation, platelet function and coagulation factors as a result of its inhibition on the plasmin. Methods. WOMAN ETAPlaT is a sub-study of the World Maternal Antifibrinolitic trial (WOMAN trial). All adult women clinically diagnosed with PPH after a vaginal delivery or caesarean section, are eligible for inclusion in the study. Blood samples will be collected at the baseline and 30 minutes after the first dose of study treatment is given. Platelet function will be evaluated in whole blood immediately after sampling with Multiplate® tests (ADPtest and TRAPtest). Thrombin generation, fibrinogen, D-dimer, and coagulation factors vW, V and VIII will be analysed using platelet poor plasma. Results. Recruitment to WOMAN ETAPlaT started on 04 November 2013 and closed on 13 January 2015, during this time 188 patients were recruited. The final participant follow-up was completed on 04 March 2015. This article introduces the statistical analysis plan for the study, without reference to unblinded data. Conclusion. The data from this study will provide evidence for the effect of TXA on thrombin generation, platelet function and coagulation factors in women with PPH. Trial registration: ClinicalTrials.gov Identifier: NCT00872469; ISRCTN76912190
Ischnocodia annulus (Fabricius) (Coleoptera: Chrysomelidae: Cassidinae): Primeiro Registro para a Mata Atlântica Brasileira, Novas Plantas Hospedeiras e Considerações sobre o Período de Ocorrência
Authors: Lopes Marta Custodio , Antunes Nicanor Tiago Bueno 
Published: December 2016

O objetivo deste trabalho foi realizar novo registro de ocorrência para Ischnocodia annulus (Fabricius), além de fornecer informações sobre plantas hospedeiras e período de ocorrência da espécie. Os besouros foram observados em um fragmento florestal no Oeste do Paraná; trata-se do primeiro registro da espécie para a região Sul do Brasil e para a Mata Atlântica brasileira. Foram notificadas duas novas plantas hospedeiras para a espécie: Cordia trichotoma Vell. Arráb. ex Steud e Cordia eucalyculata Vell. (Boraginaceae). Os imaturos emergiram na segunda quinzena de setembro e os últimos adultos foram observados no início de dezembro. A emergência dos imaturos ocorre na mesma época que plântulas de C. trichotoma germinam em grande quantidade.Ischnocodia annulus (Fabricius) (Coleoptera: Chrysomelidae: Cassidinae): First Record for Brazilian Atlantic Forest, New Host Plants and Considerations about Period of Occurrence Abstract. The objective of this study was making new record of Ischnocodia annulus (Fabricius) and provides information about host plants period of occurrence. The beetles were observed in a forest fragment in Paraná western and this is the first record of I. annulus for the southern region of Brazil and for the brazilian Atlantic Forest. Two new host plants were notified: Cordia trichotoma Vell. Arráb. ex Steud and Cordia eucalyculata Vell. (Boraginaceae). The immatures emerged in the second half of September and the last adults were observed in early December. The emergence of immature occurs at the same time that C. trichotoma seedlings germinate aplenty.
Treatment of multiple wounds of aplasia cutis congenita on the lower limb: a case report
Authors: Cherubino M. , Maggiulli F. , Dibartolo R. , Valdatta L. 
Published: December 2016

Introduction: Aplasia cutis congenita (ACC) is a rare congenital disease, characterised by absence of skin, that can affect different parts of the body. ACC is more frequent on the scalp, but can involve limbs, with partial-or total-thickness loss of skin, with a functional impairment of the joint affected. There is no clear indication for surgical or conservative treatment, therefore, it would be helpful to find scientific support for the optimal treatment strategies. Clinical presentation: We present a case of a newborn female, with six wounds on the inferior limbs, treated with conservative therapy. To prevent infection and promote healing, the defects were kept moist and covered with non-adherent/antimicrobical dressings. Complete wound healing occurred in eight weeks. The duration of follow-up was three years. Results: In this kind of ACC with superficial partial-thickness wounds of the lower limbs, conservative treatment was successful. The same conservative treatment can be proposed for larger or deeper wounds of lower limbs with no involvement of underlying structures, with delayed scar excision after the complete growth of the patient. ACC scar excision could require complex tissue rearrangement, tissue expansion, or skin grafting. Conclusion: In the evaluation for treatment of ACC of inferior limbs, even in cases of total-thickness skin loss, even on joints, the conservative approach could be considered as the first choice. Declaration of interest: None of the authors has a financial interest in any of the products, devices, or drugs mentioned in this manuscript.
Inspection of general practice and the role of the practice nurse
Authors: Biscoe Elaine 
Published: December 2016

In the last two years, the Care Quality Commission has completed its first round of inspections of general practice, with 83% being found good or outstanding. The CQC's Elaine Biscoe explains the lessons learned and the strategy for the future The Care Quality Commission (CQC) is coming to the end of its first round of comprehensive inspections of GP practices and planning the future strategy. The following article will summarise its findings so far with particular relevance for nursing in primary care, and then outline the current plans for the new model of regulation.
Combined Count- and Size-Based Analysis of Maternal Plasma DNA for Noninvasive Prenatal Detection of Fetal Subchromosomal Aberrations Facilitates Elucidation of the Fetal and/or Maternal Origin of the Aberrations
Authors: Yu Stephanie C Y , Jiang Peiyong , Chan K C Allen , Faas Brigitte H W , Choy Kwong W , Leung Wing C , Leung Tak Y , Lo Y M Dennis , Chiu Rossa W K 
Published: December 2016

Abstract

BACKGROUND
Noninvasive prenatal detection of fetal subchromosomal copy number aberrations (CNAs) can be achieved through massively parallel sequencing of maternal plasma DNA. However, when a mother herself is a carrier of a CNA, one cannot discern if her fetus has inherited the CNA. In addition, false-positive results would become more prevalent when more subchromosomal regions are analyzed.

METHODS
We used a strategy that combined count- and size-based analyses of maternal plasma DNA for the detection of fetal subchromosomal CNAs in 7 target regions for 10 test cases.

RESULTS
For the 5 cases in which CNAs were present only in the fetus, the size-based approach confirmed the aberrations detected by the count-based approach. For the 5 cases in which the mother herself carried an aberration, we successfully deduced that 3 of the fetuses had inherited the aberrations and that the other 2 fetuses had not inherited the aberrations. No false positives were observed in this cohort.

CONCLUSIONS
Combined count- and size-based analysis of maternal plasma DNA permits the noninvasive elucidation of whether a fetus has inherited a CNA from its mother who herself is a carrier of the CNA. This strategy has the potential to improve the diagnostic specificity of noninvasive prenatal testing.
BRCA Testing by Single-Molecule Molecular Inversion Probes
Authors: Neveling Kornelia , Mensenkamp Arjen R , Derks Ronny , Kwint Michael , Ouchene Hicham , Steehouwer Marloes , van Lier Bart , Bosgoed Ermanno , Rikken Alwin , Tychon Marloes , Zafeiropoulou Dimitra , Castelein Steven , Hehir-Kwa Jayne , Tjwan Thung Djie , Hofste Tom , Lelieveld Stefan H , Bertens Stijn M M , Adan Ivo B J F , Eijkelenboom Astrid , Tops Bastiaan B , Yntema Helger , Stokowy Tomasz , Knappskog Per M , Høberg-Vetti Hildegunn , Steen Vidar M , Boyle Evan , Martin Beth , Ligtenberg Marjolijn J L , Shendure Jay , Nelen Marcel R , Hoischen Alexander 
Published: December 2016

Abstract

BACKGROUND
Despite advances in next generation DNA sequencing (NGS), NGS-based single gene tests for diagnostic purposes require improvements in terms of completeness, quality, speed, and cost. Single-molecule molecular inversion probes (smMIPs) are a technology with unrealized potential in the area of clinical genetic testing. In this proof-of-concept study, we selected 2 frequently requested gene tests, those for the breast cancer genes BRCA1 and BRCA2, and developed an automated work flow based on smMIPs.

METHODS
The BRCA1 and BRCA2 smMIPs were validated using 166 human genomic DNA samples with known variant status. A generic automated work flow was built to perform smMIP-based enrichment and sequencing for BRCA1, BRCA2, and the checkpoint kinase 2 (CHEK2) c.1100del variant.

RESULTS
Pathogenic and benign variants were analyzed in a subset of 152 previously BRCA-genotyped samples, yielding an analytical sensitivity and specificity of 100%. Following automation, blind analysis of 65 in-house samples and 267 Norwegian samples correctly identified all true-positive variants (&gt;3000), with no false positives. Consequent to process optimization, turnaround times were reduced by 60% to currently 10–15 days. Copy number variants were detected with an analytical sensitivity of 100% and an analytical specificity of 88%.

CONCLUSIONS
smMIP-based genetic testing enables automated and reliable analysis of the coding sequences of BRCA1 and BRCA2. The use of single-molecule tags, double-tiled targeted enrichment, and capturing and sequencing in duplo, in combination with automated library preparation and data analysis, results in a robust process and reduces routine turnaround times. Furthermore, smMIP-based copy number variation analysis could make independent copy number variation tools like multiplex ligation-dependent probes amplification dispensable.
The study of the reliability and validity of Lifespan Sibling Relationship Scale in young adults<p>Yaşam Boyu Kardeş İlişkileri Ölçeğinin genç yetişkinlerde geçerlik ve güvenirlik çalışması
Authors: Öz Soysal Fatma Selda , Yurdabakan İrfan , Uz Baş Aslı , Aysan Ferda 
Published: December 2016

The purpose of this study was to explore validity and reliability of the Lifespan Sibling Relationship Scale (LSRS) which was developed by Riggio (2000) in a sample of young adults. The research has been conducted on 510 (339 female, 171 male) university students who attended pedagogical formation training at Dokuz Eylül University and fourth grade students who attended various departments of Faculty of Education. The six factor structure of the LSRS was tested by means of confirmatory factor analysis method. The findings indicated that the six-factor model is supported by using the confirmatory factor analysis. The reliability of LSRS was examined with test-retest reliability and internal consistency methods. Results showed that the scale can be used for measuring sibling relationships in young adults. ÖzetBu araştırmanın amacı Riggio (2000) tarafından geliştirilen Yaşam Boyu Kardeş İlişkileri Ölçeği’nin (YBKİÖ) genç yetişkinlerden oluşan bir örneklemde geçerlik ve güvenirlik çalışmasını yapmaktır. Araştırma, Dokuz Eylül Üniversitesi’nde pedagojik formasyon eğitimi alan ve Eğitim Fakültesi’nin çeşitli bölümlerine devam eden dördüncü sınıf öğrencileri olan toplam 510 (339 kız, 171 erkek) öğrenci ile yürütülmüştür. YBKİÖ’nün altı faktörlü yapısı doğrulayıcı faktör analizi yöntemi ile test edilmiştir. Elde edilen sonuçlar, YBKİÖ’nün altı faktörlü yapısının doğrulandığını göstermiştir. Ölçeğin güvenirlik çalışması, test-tekrar test güvenirlik katsayısı ve iç tutarlılık katsayısı ile hesaplanmıştır. Elde edilen sonuçlar, YBKİÖ’nün genç yetişkinlerde kardeş ilişkilerinin değerlendirmek üzere geçerli ve güvenilir bir ölçme aracı olarak kullanılabileceğini göstermiştir.
Damage indication of 2′, 7′-dichlorofluorescein for epoxy polymer and the effect of water on its damage indicating ability
Authors: Guo Yakun , Zhao Pengxiang , Wang Xiaofang , Xu Duigong , Zhong Jingrong , Yue Guozong , Shuai Maobing 
Published: December 2016

Abstract
Epoxy polymer with damage indicating ability was very usable for ships and bridges to detect the cracks at an early stage and to prevent corrosion. 2′, 7′-dichlorofluorescein (DCF), as a damage indicator, was used to report the mechanical damage of epoxy-amine polymer by a strong color change from a light yellow to bright red due to the molecular structure transition from the acid molecular form to the base ion form. The effect of water on damage indicator and damaged epoxy-amine polymer film was evaluated by an immersion test and the properties were characterized by ultraviolet-visible spectrophotometry (UV-Vis), scanning electronic microscopy (SEM), energy dispersive X-ray spectrometer (EDS), zeta potential and thermal gravimetric analysis (TGA). The results showed that DCF was an easy, stable and permanent indicator for epoxy-amine polymer and the water only had a slight influence on the indication stability of damaged epoxy polymer.