bluuebunny/crossref_metadata_2025_split huggingface dataset:
First-time mothers’ breast-feeding maintenance: role of experiences and changes in maternal perceptions
Authors: Schafer Ellen J , Campo Shelly , Colaizy Tarah T , Mulder Pamela J , Breheny Patrick , Ashida Sato 
Published: September 2017

AbstractObjectiveBreast-feeding initiation rates have increased in the USA; however, maintenance of breast-feeding for recommended durations is low. The objective of the present study was to identify factors that may facilitate breast-feeding for longer durations among first-time mothers, including physiological and social experiences and changes in maternal perceptions.DesignSurvival analysis and linear regression methods were used to explore the relationship between experiences and breast-feeding duration, and the possible mediating effect of changes in maternal perceptions.SettingSecondary data from the Infant Feeding Practices Study II, conducted in the USA between 2005 and 2007.SubjectsData from 762 first-time mothers who ever breast-fed were analysed.ResultsExperiencing trouble with baby’s latch, problems with milk flow/supply and painful breast-feeding were significantly associated with breast-feeding duration (64, 26 and 36 % shorter duration, respectively). Meanwhile, positive changes in perception with respect to breast-feeding self-efficacy, opinion about infant feeding and belief about breast milk were associated with 16–27 % longer duration. Furthermore, changes in perception were observed to partially mediate the impact of physiological experiences on breast-feeding duration.ConclusionsPerceptions of breast-feeding self-efficacy, beliefs and opinions can change over time and are influenced by breast-feeding experiences. The combined effect of experience and perception plays a key role in influencing breast-feeding duration. Future research should explore interventions to maintain or improve these perceptions while accounting for physiological experiences to support breast-feeding for recommended durations among first-time mothers.
Legal advocacy as a tool to advance Roma Health
Authors: Ezer Tamar , Abdikeeva Alphia , McKee Martin 
Published: September 2017

AbstractRoma in central Europe face many violations of their rights, including those that impede their access to high-quality health care. Legal frameworks have the potential to address these violations, whereas legal advocacy services offer a means for enforcement of rights. We undertook key informant interviews with Roma civil society organisations and selected knowledgeable individuals in Macedonia, Romania and Serbia to identify lessons from the development of these services. Achievements were reported in four areas. Legal empowerment, with employment of Roma paralegals was especially effective, increasing awareness of the ability to challenge violations. Documentation of human rights violations is an important basis for advocacy, but does not guarantee redress, and may work best in combination with legal empowerment or international pressure. Strategic litigation can play a key role in removing bureaucratic obstacles that prevent Roma from exercising their right to access to health care. Progress in changing the narrative on Roma in the mainstream media has been limited but examples of good practice exist. Although much remains to be done, Roma groups report that legal advocacy has strengthened their ability to challenge rights violations, thereby enhancing their ability to access effective and responsive care.
Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway
Authors: Chen Erman E M , Zhang Wei , Ye Chenyi C Y , Gao Xiang , Jiang Liangjun L J , Zhao Tengfei T F , Pan Zhijun Z J , Xue Deting D T 
Published: September 2017

AbstractSirtuin 7 (SIRT7) is a NAD+-dependent deacetylase in the sirtuin family. In a previous study, human bone marrow mesenchymal stem cells (hBMSCs) with reduced SIRT7 activity were developed to evaluate the effect of SIRT7 on osteogenesis. SIRT7 knockdown significantly enhanced osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Additionally, SIRT7 knockdown upregulated β-catenin. The enhanced osteogenesis due to SIRT7 knockdown was partially rescued by a Wnt/β-catenin inhibitor. Furthermore, SIRT7 knockdown hBMSCs combined with a chitosan scaffold significantly promoted bone formation in a rat tibial defect model, as determined by imaging and histological examinations. These findings suggest that SIRT7 has an essential role in osteogenic differentiation of hBMSCs, partly by activation of the Wnt/β-catenin signaling pathway.
An in planta biolistic method for stable wheat transformation
Authors: Hamada Haruyasu , Linghu Qianyan , Nagira Yozo , Miki Ryuji , Taoka Naoaki , Imai Ryozo 
Published: September 2017

AbstractThe currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta wheat transformation method using biolistic DNA delivery without callus culture or regeneration. Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under a microscope and subjected to bombardment with different-sized gold particles coated with the GFP gene construct, introducing DNA into the L2 cell layer. Bombarded embryos were grown to mature, stably transformed T0 plants and integration of the GFP gene into the genome was determined at the fifth leaf. Use of 0.6-µm particles and 1350-psi pressure resulted in dramatically increased maximum ratios of transient GFP expression in SAMs and transgene integration in the fifth leaf. The transgene was integrated into the germ cells of 62% of transformants, and was therefore inherited in the next generation. We successfully transformed the model wheat cultivar ‘Fielder’, as well as the recalcitrant Japanese elite cultivar ‘Haruyokoi’. Our method could potentially be used to generate stable transgenic lines for a wide range of commercial wheat cultivars.
One‐step synthesis of oxidation‐resistant Cu@Ag core–shell nanoparticles
Authors: Yuan Yujia , Xia Hui , Chen Yu , Xie Ding 
Published: September 2017

One‐step synthesis of wet chemical method is proposed for the preparation of core–shell nanostructures of copper and silver. This facile and environment friendly fabrication simplifies the surface treatment of copper particles to avoid copper core to be oxidised before coating silver. The thermal stability against oxidation and electrical properties of Cu@Ag core–shell nanoparticles are investigated. The results show that Cu@Ag core–shell nanoparticles possess high spherical degree, and the silver is evenly coated on the surface of copper nanoparticles. The synthesised spherical Cu@Ag core–shell nanoparticles exhibit excellent thermal stability at the high temperature of 300°C. After annealing at 200°C for 30 min, the sheet resistance of the spherical Cu@Ag core–shell nanoparticles (Ag content 7.68 wt%) is extremely low (69.82 μΩ cm).
Effectiveness of “Indocyanine Green Dye Clamp Test” in Selecting Perforators for Muscle-Sparing-2 Transverse Rectus Abdominis Myocutaneous Flaps in Breast Reconstruction
Authors: Okumura Seiko , Takeishi Meisei , Nakamura Ryota , Kakutani Satoshi , Hyodo Ikuo , Kamei Yuzuru 
Published: September 2017

Introduction In breast reconstruction for cases in which tissue from zones 1 to 4 is required or a lower midline scar exists, it is possible to predict the need for vascular anastomosis with a vascular pedicle on the contralateral side if the dominant territory of each perforator can be identified in advance. In addition, it is possible to determine whether a single- or multiple-perforator-based flap is needed. We, therefore, developed the “indocyanine green dye (ICG) clamp test” to select perforators for breast reconstruction.
Methods The blood flow of a perforator was blocked using a microvascular clamp when determining its inclusion in a flap. The clamps were released after the enlargement of the ICG fluorescence imaging range. The enlarged imaging range was then observed to determine whether an additional pedicle was required.
Subjects One hundred thirty-two breast reconstruction procedures had been performed using the free muscle-sparing-2 transverse rectus abdominis myocutaneous (MS2 TRAM) flap method from May 2012 to December 2015. The population of the present study included 29 of these cases in which the selection of perforators was deemed necessary.
Results We investigated the cases in which anastomosis of the contralateral vascular pedicle was unnecessary. It was possible to preserve the medial muscle in 79.3% of the cases using our procedure.
Conclusion Our newly developed ICG clamp test was useful in selecting perforators for MS2 TRAM flaps and facilitated the performance of minimally invasive surgery.
Thomas Vinterberg: Die Kommune
Authors: Tilch-Bauschke Edeltraud 
Published: September 2017

Der 1969 in Kopenhagen geborene Regisseur Thomas Vinterberg wurde 1993 durch das Familien- und Missbrauchsdrama „Das Fest“ bekannt. Sein
jüngster Film „Die Kommune“ wurde 2016 im Wettbewerb der Berlinale gezeigt. Vinterberg, der selbst vom 9.−17. Lebensjahr mit seinen
Eltern in einer Kommune aufwuchs, weiß um die Problematik einer solchen Lebensform.
Das Atmen erleichtern
Authors: Heppner Hans Jürgen 
Published: September 2017

Zusammenfassung
Beatmungstherapie beim geriatrischen Patienten Mit zunehmendem Alter verändern sich auch Atmungssystem, Muskelkraft und Muskelmasse
des Menschen. Entscheidende Faktoren, die es zu bedenken gilt, wenn ein Patient beatmungstherapeutische Unterstützung braucht.
Integrating splice-isoform expression into genome-scale models characterizes breast cancer metabolism
Authors: Angione Claudio 
Published: September 2017

Abstract

Motivation
Despite being often perceived as the main contributors to cell fate and physiology, genes alone cannot predict cellular phenotype. During the process of gene expression, 95% of human genes can code for multiple proteins due to alternative splicing. While most splice variants of a gene carry the same function, variants within some key genes can have remarkably different roles. To bridge the gap between genotype and phenotype, condition- and tissue-specific models of metabolism have been constructed. However, current metabolic models only include information at the gene level. Consequently, as recently acknowledged by the scientific community, common situations where changes in splice-isoform expression levels alter the metabolic outcome cannot be modeled.

Results
We here propose GEMsplice, the first method for the incorporation of splice-isoform expression data into genome-scale metabolic models. Using GEMsplice, we make full use of RNA-Seq quantitative expression profiles to predict, for the first time, the effects of splice isoform-level changes in the metabolism of 1455 patients with 31 different breast cancer types. We validate GEMsplice by generating cancer-versus-normal predictions on metabolic pathways, and by comparing with gene-level approaches and available literature on pathways affected by breast cancer. GEMsplice is freely available for academic use at https://github.com/GEMsplice/GEMsplice_code. Compared to state-of-the-art methods, we anticipate that GEMsplice will enable for the first time computational analyses at transcript level with splice-isoform resolution.

Availability and implementation
https://github.com/GEMsplice/GEMsplice_code

Supplementary information
Supplementary data are available at Bioinformatics online.
Enhanced guide-RNA design and targeting analysis for precise CRISPR genome editing of single and consortia of industrially relevant and non-model organisms
Authors: Mendoza Brian J , Trinh Cong T 
Published: September 2017

Abstract

Motivation
Genetic diversity of non-model organisms offers a repertoire of unique phenotypic features for exploration and cultivation for synthetic biology and metabolic engineering applications. To realize this enormous potential, it is critical to have an efficient genome editing tool for rapid strain engineering of these organisms to perform novel programmed functions.

Results
To accommodate the use of CRISPR/Cas systems for genome editing across organisms, we have developed a novel method, named CRISPR Associated Software for Pathway Engineering and Research (CASPER), for identifying on- and off-targets with enhanced predictability coupled with an analysis of non-unique (repeated) targets to assist in editing any organism with various endonucleases. Utilizing CASPER, we demonstrated a modest 2.4% and significant 30.2% improvement (F-test, P &lt; 0.05) over the conventional methods for predicting on- and off-target activities, respectively. Further we used CASPER to develop novel applications in genome editing: multitargeting analysis (i.e. simultaneous multiple-site modification on a target genome with a sole guide-RNA requirement) and multispecies population analysis (i.e. guide-RNA design for genome editing across a consortium of organisms). Our analysis on a selection of industrially relevant organisms revealed a number of non-unique target sites associated with genes and transposable elements that can be used as potential sites for multitargeting. The analysis also identified shared and unshared targets that enable genome editing of single or multiple genomes in a consortium of interest. We envision CASPER as a useful platform to enhance the precise CRISPR genome editing for metabolic engineering and synthetic biology applications.

Availability and implementation
https://github.com/TrinhLab/CASPER.

Supplementary information
Supplementary data are available at Bioinformatics online.
Autoimmune Progesterone Dermatitis Presenting as Stevens-Johnson Syndrome
Authors: Drayer Sara M. , Laufer Larry R. , Farrell Maureen E. 
Published: September 2017

BACKGROUND:
Autoimmune progesterone dermatitis is an uncommon disease presenting with cyclical skin eruptions corresponding with the menstrual cycle luteal phase. Because symptoms are precipitated by rising progesterone levels, treatment relies on hormone suppression.

CASE:
A 22-year-old nulligravid woman presented with symptoms mistaken for Stevens-Johnson syndrome. A cyclic recurrence of her symptoms was noted, and the diagnosis of autoimmune progesterone dermatitis was made by an intradermal progesterone challenge. After 48 months, she remained refractory to medical management and definitive surgical treatment with bilateral oophorectomy was performed.

CONCLUSION:
Autoimmune progesterone dermatitis is a challenging diagnosis owing to its rarity and variety of clinical presentations. Treatment centers on suppression of endogenous progesterone and avoidance of exogenous triggers. When these modalities fail, surgical management must be undertaken.
Spinal Gout Without Spinal Symptom in a Junior School Student
Authors: Wu Danbin , Ma Jiajian , Li Songsong , Zhao Jinwei , Li Ling 
Published: September 2017

Study Design.
Case report.

Objective.
We report a case of a 16-year-old boy with intermittent and migratory polyarthralgia, who made a diagnostic dilemma.

Summary of Background Data.
Spinal involvement without spinal symptom in gout seems to be rare. However, the relationship of spinal gout to symptoms is poorly understood.

Methods.
Description of the case report.

Results.
Laboratory findings cannot explain his symptoms; however, a computed tomography of the pelvis revealed the presence of space-occupying lesion involving the left side of spine at L5-S1 level, and the later biopsy revealed that was a urate crystal, which help us make the diagnosis of spinal gout.

Conclusion.
Gout can be a cunning disease which has various manifestations, and spinal involvement can be asymptomatic.

Level of Evidence: 5
Correlation Between Exercise Capacity and Quality of Life in Patients With Cardiac Disease
Authors: Andersen Karoline Stentoft , Laustsen Sussie , Petersen Annemette Krintel 
Published: September 2017

Purpose:
Patients are referred to exercise-based cardiac rehabilitation (ECR) to increase exercise capacity and health-related quality of life (HRQOL) and thereby reduce risk of morbidity and mortality. The purpose of this study was to examine the correlation between exercise capacity and HRQOL. Furthermore, this study examined whether improvements in HRQOL were directly related to improvements in exercise capacity.

Methods:
The study included 277 patients participating in ECR. HRQOL was assessed using the Medical Outcomes Study 36-item Short Form Health Survey (SF-36), and exercise capacity was measured as peak oxygen uptake (

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o
2peak) and oxygen uptake (

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o
2) at the ventilatory threshold (VTh). Patients were examined before and after completion of an 8-wk ECR program.

Results:
Analyses at baseline showed a significant correlation between

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o
2peak and VTh and physical functioning (PF), role limitations because of physical problems (RP), general health perceptions (GH), vitality (VT), and physical component summary (PCS) on the SF-36; PF was the only dimension showing a moderate correlation (>0.40 Spearman ρ) with

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o
2peak. The follow-up analyses demonstrated a significant correlation between changes in

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2peak and changes in PF, RP, VT, and MH. Changes in

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o
2 peak explained 4% of the changes in the PF and VT scores.

Conclusion:
The correlations between exercise capacity and HRQOL were weak and varied considerably among patients. The ECR program improved both exercise capacity and HRQOL, but it was not necessarily the same patients who improved both parameters. Therefore, it is recommended to use separate objective measures and patient-reported outcomes when evaluating the effect of ECR.
Assessing Readability and Reliability of Online Patient Information Regarding Vestibular Schwannoma
Authors: Spiers Harry , Amin Nikul , Lakhani Raj , Martin Andrew J. , Patel Parag M. 
Published: September 2017

Hypothesis:
The aim of this study is to objectively assess the quality and readability of websites related to vestibular schwannomas.

Background:
Patients are increasingly seeking information on confirmed or suspected diagnoses through the Internet. Clinicians are often concerned regarding the accuracy, quality, and readability of web-based sites.

Methods:
Online information relating to vestibular schwannoma was searched using the three most popular search engines. The terms “acoustic neuroma” and “vestibular schwannoma” were used. The top 50 results from each site were assessed for readability using the Flesch-Kincaid Grade Level, Flesch Reading Ease Score, and the Gunning-Fog Index. Quality of website information was scored using the DISCERN tool.

Results:
Of 300 search results analyzed, 58 separate appropriate websites were identified. The mean readability score using Flesch-Kincaid Grade Level was 10.27 (95% confidence interval [CI] 9.84–10.70). The mean Flesch Reading Ease Score was 48.75 (95% CI 46.57–50.92). The Gunning-Fog Index was 13.40 (95% CI 12.92–13.89). These scores equate to someone finishing secondary school/first year university student. DISCERN scores were highly variable but consistently demonstrated great variability in quality of information.

Conclusion:
Online patient information on vestibular schwannoma is highly variable in quality. Although there are a wide range of different websites easily available to patients on their condition and its treatment options, the information is written at a difficult level which may exceed the understanding level of many patients as it is written at a higher than average level of expected reading ability.
Sexually Transmitted Infection Testing of HIV-Positive Medicare and Medicaid Enrollees Falls Short of Guidelines
Authors: Landovitz Raphael J. , Gildner Jennifer L. , Leibowitz Arleen A. 
Published: September 2017

Background
Men who have sex with men with HIV have high sexually transmitted infection (STI) incidence. Thus, the Centers for Disease Control and Prevention (CDC) recommends at least yearly STI screening of HIV-infected individuals.

Methods
We calculated testing rates for syphilis, chlamydia, and gonorrhea among HIV-positive Californians with Medicare or Medicaid insurance in 2010. Logistic regressions estimated how testing for each bacterial STI relates to demographic and provider factors.

Results
Fewer than two-thirds of HIV-positive Medicare and fewer than three-quarters of Medicaid enrollees received a syphilis test in 2010. Screenings for chlamydia or gonorrhea were less frequent: approximately 30% of Medicare enrollees were tested for chlamydia or gonorrhea in 2010, but higher proportions of Medicaid enrollees were tested (45%–46%). Only 34% of HIV-positive Medicare enrollees who were tested for syphilis were also screened for chlamydia or gonorrhea on the same day. Nearly half of Medicaid enrollees were tested for all 3 STIs on the same day. Patients whose providers had more HIV experience had higher STI testing rates.

Conclusions
Testing rates for chlamydia and gonorrhea infection are low, despite the increase in these infections among people living with HIV and their close association with HIV transmission. Interventions to increase STI testing include the following: prompts in the medical record to routinely conduct syphilis testing on blood drawn for viral load monitoring, opt-out consent for STI testing, and provider education about the clinical importance of STIs among HIV-positive patients. Last, it is crucial to change financial incentives that discourage nucleic acid amplification testing for rectal chlamydia and gonorrhea infections.
Integrative structural Investigation on the architecture of human Importin4_histone H3/H4_Asf1a complex and its histone H3 tail binding
Authors: Yoon Jungmin , Kim Seung Joong , An Sojin , Leitner Alexander , Jung Taeyang , Aebersold Ruedi , Hebert Hans , Cho Uhn-Soo , Song Ji-Joon 
Published: September 2017

AbstractImportin4 transports histone H3/H4 in complex with Asf1a to the nucleus for chromatin assembly. Importin4 recognizes the nuclear localization sequence located at the N-terminal tail of histones. Here, we analyzed the structures and interactions of human Importin4, histones and Asf1a by cross-linking mass spectrometry, X-ray crystallography, negative-stain electron microscopy, small-angle X-ray scattering and integrative modeling. The XL-MS data showed that the C-terminal region of Importin4 interacts extensively with the histone H3 tail. We determined the crystal structure of the C-terminal region of Importin4 bound to the histone H3 peptide, thus revealing that the acidic path in Importin4 accommodates the histone H3 tail and that histone H3 Lys14 is the primary residue interacting with Importin4. Furthermore, the molecular architecture of the Importin4_histone H3/H4_Asf1a complex was produced through an integrative modeling approach. Overall, this work provides structural insights into how Importin4 recognizes histones and their chaperone complex.