bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface Wrinkling and Porosity of Polymer Particles toward Biological and Biomedical Applications
Authors: Visaveliya Nikunjkumar R. , Leishman Christopher W. , Ng Kara , Yehya Nicolas , Tobar Nelson , Eisele Dorthe M. , Köhler Johann Michael 
Published: November 2017

AbstractPolymer particles are promising particulate materials for renowned biomedical applications such as targeted drug delivery, tissue engineering, and biosensing. Surface properties of the polymer particles are of key importance for biomedical applications because they directly interact with biological systems. Particularly, wrinkled as well as porous surfaces possess an enhanced ability for cell attachment without any additional chemical modification. Therefore, a key objective is to fabricate the particles with desired degree of wrinkles and porosity. Many methods such as solvent evaporation, plasma treatment, emulsion instability, and electrospraying are being employed for the generation of porous, wrinkled and/or textured surfaces. Advantageously, an application of microfluidics can support the induction of surface instabilities on droplets in a case of droplet‐based systems. Furthermore, microfluidics allows tuning of size and shape of the generated droplets as well as particles with desired surface textures. In this minireview article, surface characteristics (especially surface wrinkles and porosity) of the hydrophobic and hydrophilic polymer particles are presented for the potential applications toward biological as well as biomedical field. In addition, the impact of microfluidics is highlighted in order to produce the polymer particles of functional surface properties.
Charge and Discharge Processes and Sodium Storage in Disodium Pyridine‐2,5‐Dicarboxylate Anode—Insights from Experiments and Theory
Authors: Padhy Harihara , Chen Yingqian , Lüder Johann , Gajella Satyanarayana Reddy , Manzhos Sergei , Balaya Palani 
Published: November 2017

AbstractA combined experimental and computational study of disodium pyridine‐2,5‐dicarboxylate (Na2PDC) is presented exploring the possibility of using it as a potential anode for organic sodium‐ion batteries. This electrode material can reversibly insert/release two Na cations per formula unit, resulting in high reversible capacity of 270 mA h g−1 (236 mA h g−1 after accounting for the contribution from Super P carbon) with excellent cyclability 225 mA h g−1, with retention of 83% capacity after 100 cycles, and good rate performance with reversible capacity of 138 mA h g−1 at a 5 C rate. The performance of disodium pyridine dicarboxylate is therefore found to be superior to that of the related and well investigated disodium terephthalate. The material shows two voltage plateaus at about 0.6 V up to Na2+1PDC and then 0.4 V up to full sodiation, Na2+2PDC. The first plateau is attributed to the coordination of inserted Na to nitrogen atoms with bond formation, i.e., a different mechanism from the terephthalate analog. The subsequent plateau is due to coordination to the carboxylic groups.
Long Cycle Life, Low Self‐Discharge Sodium–Selenium Batteries with High Selenium Loading and Suppressed Polyselenide Shuttling
Authors: Wang Hui , Jiang Yang , Manthiram Arumugam 
Published: November 2017

AbstractThe use of selenium as a cathode in rechargeable sodium–selenium batteries is hampered by low Se loading, inferior electrode kinetics, and polyselenide shuttling between the cathode and anode. Here a high‐performance sodium–selenium cell is presented by coupling a binder‐free, self‐interwoven carbon nanofiber–selenium cathode with a light‐weight carbon‐coated bifunctional separator. With this strategy, electrodes with a high Se mass loading (4.4 mg cm−2) render high reversible capacities of 599 mA h g−1 at 0.1C rate and 382 mA h g−1 at 5C rate. In addition, this novel cell offers good shelf‐life with a low self‐discharge, retaining 93.4% of its initial capacity even after resting for six months. As evidenced by experimental and density functional theory analysis, the remarkable dynamic (cycle life) and static (shelf‐life) stabilities originate from the high electrical conductivity, improved Na‐ion accessibility through the 3D interconnected open channels, and highly restrained polyselenide shuttle. The results demonstrate the viability of high‐performance sodium–selenium batteries with high selenium loading.
Firm reputation, advertising investment, and price premium: The role of collective brand membership in high‐quality wines
Authors: Sellers‐Rubio Ricardo , Mas‐Ruiz Francisco , Sancho‐Esper Franco 
Published: November 2017

AbstractResearch has recognized that the price of an experience good is not only determined by firm and collective reputation, which act as proxies of firm product quality, but also by the firm's advertising investment. This study examines whether pricing these goods as a function of firm advertising investment and firm reputation depends on a collective, versus noncollective, brand strategy. The central assumption of this article is that a collective brand can increase perceived differentiation among the individual brands associated with it, and thus, that collective brand membership can moderate the effects of firm advertising investment and firm reputation on product price. The results obtained from a panel of companies in an experience‐goods industry (i.e., wineries) between 1999 and 2013 show that the relative effect of collective brand membership on product price is higher when the company has higher advertising investment and a higher reputation. [EconLit citations:Q11, Q13, M37]
Adapting the T0‐T4 implementation science model to occupational health and safety in agriculture, forestry, and fishing: A scoping review
Authors: Tinc Pamela J. , Gadomski Anne , Sorensen Julie A. , Weinehall Lars , Jenkins Paul , Lindvall Kristina 
Published: November 2017

BackgroundDespite much research to develop life‐saving innovations for the agriculture, forestry, and fishing workforce, these populations continue to face the highest fatal and non‐fatal injury rates in the United States, as many of these solutions are not fully adopted.MethodsA scoping review was conducted to provide an overview of research to practice efforts in this field. The language used to describe these initiatives, the utility of the NIH T0‐T4 model, and the progress along the research to practice continuum were examined.ResultsFourteen eligible references demonstrated that progress in implementation science is lacking and that there is little consistency in how researchers apply the T0‐T4 model; thus, a new model is presented.ConclusionsResearchers in this field face several challenges when moving from research to practice. While some challenges are addressed with the proposed model, additional resources and infrastructure to support such initiatives are necessary.
A retrospective analysis of cardiometabolic health in a large cohort of truck drivers compared to the American working population
Authors: Thiese Matthew S. , Hanowski Richard J. , Moffitt Gary , Kales Stefanos N. , Porter Richard. J. , Ronna Brenden , Hartenbaum Natalie , Hegmann Kurt T. 
Published: November 2017

BackgroundTruck drivers face many health challenges, including lifestyle, diet, inactivity, stressors, and social support.MethodsA repeated cross‐sectional analysis compared 88,246 truck drivers with data from (n = 11 918) the National Health and Nutrition Examination Survey (NHANES). Trends over time for body mass index (BMI), high blood pressure, diabetes mellitus, heart disease, and sleep disorders were analyzed, adjusting for potential confounders.ResultsTruck drivers had significantly worsening health metrics between 2005 and 2012 compared to NHANES participants. Truck drivers were significantly more likely to be obese and morbidly obese with prevalence odds ratios (POR) and 95% confidence intervals (95%CI) of 1.99 (1.87, 2.13) and 2.34 (2.16, 2.54), respectively. Measured blood pressure, self‐reported high blood pressure, diabetes mellitus, and heart disease all significantly increased. Also, sleep disorders increased among truck drivers from 2005 to 2012 (POR = 6.55, 95%CI 5.68, 7.55).ConclusionThese data suggest disproportionate increases in poor health among truck drivers from 2005 to 2012.
Entwicklung einer DNA‐Polymerase für die direkte m6A‐Sequenzierung
Authors: Aschenbrenner Joos , Werner Stephan , Marchand Virginie , Adam Martina , Motorin Yuri , Helm Mark , Marx Andreas 
Published: November 2017

AbstractMethoden zur Analyse von RNA‐Modifikationen sind essenziell für das Forschungsfeld der Epitranskriptomik. N6‐Methyladenosin (m6A) ist die häufigste Modifikation in der mRNA von Säugetieren und erfüllt Funktionen in der Regulation der Genexpression. Techniken zur Detektion dieser Modifikation basieren derzeit auf der Anreicherung von m6A‐haltigen RNA‐Fragmenten durch Antikörper. Dieser m6A‐spezifische Schritt vor der Sequenzierung ist nötig, da die Information über die Modifikation während der reversen Transkription (RT) gelöscht wird. Um die Nachteile einer solchen indirekten Detektion zu überwinden, haben wir uns zum Ziel gesetzt, neue DNA‐Polymerasen zu entwickeln, die eine direkte m6A‐Sequenzierung ermöglichen. Zu diesem Zweck wurde ein Verfahren etabliert, das die Identifizierung von RT‐aktiven KlenTaq‐DNA‐Polymerase‐Varianten mit einer erhöhten Fehleinbaurate gegenüber m6A ermöglicht. So konnten m6A‐haltige Stellen direkt aus RNA‐Sequenzierungsdaten identifiziert werden.
Mild condition dissolution of high molecular weight cotton cellulose in 1‐butyl‐3‐methylimidazolium acetate/N,N‐dimethylacetamide solvent system
Authors: Acharya Sanjit , Hu Yang , Abidi Noureddine 
Published: November 2017

ABSTRACTTo investigate the effective dissolution of high molecular weight (MW) cellulose macromolecules at ambient conditions, cellulose (DP > 4,000) derived from cotton fiber waste was dissolved in 1‐butyl‐3‐methylimidazolium acetate (BMIMAc)/N,N‐dimethylacetamide (DMAc) solvent in this study. High MW cotton cellulose achieves a solubility between 3% and 5% cellulose concentrations using BMIMAc/DMAc solvent at ambient conditions. Rheological studies showed that all cellulose/solvent solutions displayed a shear thinning behavior. Results from the physical characterization revealed that well‐dissolved cotton cellulose exhibited highly porous structure and the crystalline structure of cotton cellulose was highly disrupted during dissolution and regeneration processes. This study is the first to report on the ability of BMIMAc/DMAc solvent system to dissolve high MW cellulose under ambient conditions, which represents an energy‐saving and environmentally friendly approach. As cellulose used in this study was derived from low quality waste cotton fibers, the potential utilization of such cotton cellulose may create a competitive market for low quality cotton and target advanced applications of cellulose‐based products for green materials and energy. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45928.
Types of value and cost in consumer–green brands relationship and loyalty behaviour
Authors: Papista Erifili , Chrysochou Polymeros , Krystallis Athanasios , Dimitriadis Sergios 
Published: November 2017

AbstractRecent literature has proposed the dynamic construct of customer value (CV) as an alternative approach to studying proenvironmental consumer purchase and loyalty behaviour. This study empirically tests and validates a model pertaining to the factors that motivate and/or hinder the development of the relationship between consumers and green brands. Findings from a survey on green brands in the detergent category indicate that a set of value dimensions and (purchase) costs have a significant influence on perceptions of CV, which, as an overall assessment construct, mediate the relationship between value dimensions and costs and loyalty to the green brand. This research also examines the moderating effect of involvement on the relationships between the 3 antecedents (value dimensions, purchase costs, and switching costs) and perceived CV. Overall, the current study offers a multiattribute understanding of consumers' relational behaviour, concluding with several value‐enhancing and cost‐minimising implications for green marketing practitioners.
The sustainability‐age dilemma: A theory of (un)planned behaviour via influencers
Authors: Johnstone Leanne , Lindh Cecilia 
Published: November 2017

AbstractThis study investigates the relation between age and sustainability awareness for consumers via the third, mediating variable of influencers to reduce the intention‐behaviour purchase gap. It proposes that traditional theories of planned behaviour are limited as they do not account for unconscious and indirect pathways to axiological change. A structural model with the 3 constructs of age, influencers, and sustainability awareness is tested with linear structural relations on a sample of 788 consumers, complemented with focus groups and interviews to generate deeper insight into the model's constructs. The results demonstrate a relationship between age and sustainability awareness, as well as between the importance of influencers for increased sustainability awareness in younger consumers, namely, millennials. This suggests that practitioners should work with influencers perceived as trustworthy to increase sustainability awareness for the millennial subset consumer group. The methodology applies a mixed approach, interpreting both qualitative and quantitative aspects, and contributes to the ethical consumerism literature with new knowledge on age differences connected to sustainability awareness, particularly highlighting on the influence of influencers for the younger generations.
Nucleophilic Water Capture or Proton Loss: Single Amino Acid Switch Converts δ‐Cadinene Synthase into Germacradien‐4‐ol Synthase
Authors: Loizzi Marianna , González Veronica , Miller David J. , Allemann Rudolf K. 
Published: November 2017

Abstractδ‐Cadinene synthase is a sesquiterpene cyclase that utilises the universal achiral precursor farnesyl diphosphate (FDP) to generate predominantly the bicyclic sesquiterpene δ‐cadinene and about 2 % germacradien‐4‐ol, which is also generated from FDP by the cyclase germacradien‐4‐ol synthase. Herein, the mechanism by which sesquiterpene synthases discriminate between deprotonation and reaction with a nucleophilic water molecule was investigated by site‐directed mutagenesis of δ‐cadinene synthase. If W279 in δ‐cadinene synthase was replaced with various smaller amino acids, the ratio of alcohol versus hydrocarbon product was directly proportional to the van der Waals volume of the amino acid side chain. DCS‐W279A is a catalytically highly efficient germacradien‐4‐ol synthase (kcat/KM=1.4×10−3 μm s−1) that produces predominantly germacradien‐4‐ol in addition to 11 % δ‐cadinene. Water capture is not achieved through strategic positioning of a water molecule in the active site, but through a coordinated series of loop movements that allow bulk water access to the final carbocation in the active site prior to product release.
A T7 RNA Polymerase Mutant Enhances the Yield of 5′‐Thienoguanosine‐Initiated RNAs
Authors: Lyon Seth , Gopalan Venkat 
Published: November 2017

AbstractSpectroscopic methods, which are used to establish RNA structure–function relationships, require strategies for post‐synthetic, site‐specific incorporation of chemical probes into target RNAs. For RNAs larger than 50 nt, the enzymatic incorporation of a nucleoside or nucleotide monophosphate guanosine analogue (G analogue) at their 5′‐end is routinely achieved by T7 RNA polymerase (T7RNAP)‐mediated in vitro transcription (IVT) of the appropriate DNA template containing a GTP‐initiating class III Φ6.5 promoter. However, when high G analogue:GTP ratios are used to bias G analogue incorporation at the 5′‐end, RNA yield is compromised. Here, we show that the use of a T7RNAP P266L mutant in IVT with 10:1 thienoguanosine (thG):GTP increased the percent incorporation and yield of 5′‐thG‐initiated precursor tRNA for a net ≈threefold gain compared to IVT with wild‐type T7RNAP. We also demonstrated that a one‐pot multienzyme approach, consisting of transcription by T7RNAP P266L and post‐transcriptional cleanup by polyphosphatase and an exonuclease, led to essentially near‐homogeneous 5′‐thG‐modified transcripts. This approach should be of broad utility in preparing 5′‐modified RNAs.
Flavin‐N5 Covalent Intermediate in a Nonredox Dehalogenation Reaction Catalyzed by an Atypical Flavoenzyme
Authors: Dai Yumin , Kizjakina Karina , Campbell Ashley C. , Korasick David A. , Tanner John J. , Sobrado Pablo 
Published: November 2017

AbstractThe flavin‐dependent enzyme 2‐haloacrylate hydratase (2‐HAH) catalyzes the conversion of 2‐chloroacrylate, a major component in the manufacture of acrylic polymers, to pyruvate. The enzyme was expressed in Escherichia coli, purified, and characterized. 2‐HAH was shown to be monomeric in solution and contained a non‐covalent, yet tightly bound, flavin adenine dinucleotide (FAD). Although the catalyzed reaction was redox‐neutral, 2‐HAH was active only in the reduced state. A covalent flavin‐substrate intermediate, consistent with the flavin‐acrylate iminium ion, was trapped with cyanoborohydride and characterized by mass spectrometry. Small‐angle X‐ray scattering was consistent with 2‐HAH belonging to the succinate dehydrogenase/fumarate reductase family of flavoproteins. These studies establish 2‐HAH as a novel noncanonical flavoenzyme.
Co‐culturing porcine normal urothelial cells, urinary bladder fibroblasts and smooth muscle cells for tissue engineering research
Authors: Zupančič Daša , Mrak Poljšak Katjuša , Kreft Mateja Erdani 
Published: November 2017

AbstractNew strategies for culturing and co‐culturing of the main types of urinary bladder cells are essential for successful establishment of biomimetic in vitro models, which could be applied for research into, and management of, diverse urological disorders. Porcine normal urothelial cells are available in nearly unlimited amounts and have many properties equivalent to human urothelial cells. In the present study, we established normal differentiated porcine urothelial cells in co‐cultures with porcine urinary bladder normal fibroblasts and/or smooth muscle cells. The optimal culture medium for establishment of differentiated urothelial cells, demonstrated by positive immunofluorescence of uroplakins, cytokeratins (CK 7, CK 20), zonula occludens 1 (ZO‐1), claudin 4, claudin 8, and E‐cadherin, was the medium composed of equal parts of Advanced Dulbecco's modified Eagle's medium (A‐DMEM) and MCDB 153 medium with physiological calcium concentration of 2.5 mM and without fetal bovine serum, named UroM (+Ca2+ − S). This medium was also proven to be suitable for culturing of bladder fibroblasts and smooth muscle cells and co‐culturing of urothelial cells with these mesenchymal cells. Urothelial cell differentiation was optimal in UroM (+Ca2+ − S) medium in all co‐culture conditions and when compared to all conditioned‐media combinations. To summarize, these strategies for culturing and co‐culturing of urinary bladder urothelial cells with mesenchymal cells could be used as new in vitro models for future basic and applicable research of the urinary bladder and thus potentially also for translational tissue engineering studies.
Aluminum Aryloxide Compounds as Very Active Catalysts for Glycidyl Methacrylate Selective Ring‐Opening Polymerization
Authors: Muñoz M. Teresa , Palenzuela Miguel , Cuenca Tomás , Mosquera Marta E. G. 
Published: November 2017

AbstractNew aluminum compounds of the type [AlXMe(OAr)]n [X=Me (1), Cl (2)] bearing the bulky ligand OAr=2,6‐bis(diphenylmethyl)‐4‐tert‐butylphenoxide were synthesized and characterized in the solid state and in solution. These compounds are mononuclear in solution. Their catalytic activity in glycidyl methacrylate (GMA) polymerization was explored. This monomer contains two different functionalities, oxirane and methacrylate, and, hence, could give functionalized polymers by ring‐opening polymerization (ROP) or vinyl polymerization. Catalysts 1 and 2 could selectively polymerize the oxirane functionality in minutes at room temperature, leaving the methacrylate group intact. Remarkably, 1 and 2 are the most active catalysts in ROP processes of GMA described so far.
Morphologically Cross‐Shaped Ru/HZSM‐5 Catalyzes Tandem Hydrogenolysis of Guaiacol to Benzene in Water
Authors: Zheng Zhaoxia , Luo Zhicheng , Zhao Chen 
Published: November 2017

AbstractHydrogenolysis of C−O bonds is an important tool for synthesis of valuable fuels and chemicals from biomass. In this contribution, we report that morphologically cross‐shaped HZSM‐5‐loaded Ru nanoparticles have demonstrated high activity in the selective hydrogenolysis of guaiacol to benzene in water with 97 % yield and a rate of 7.8 g g−1 h−1 accompanied with high durability. N2 sorption analysis showed that Ru/HZSM‐5 (cross‐shaped) had a large mesoporous surface area and pore volume for loading small and uniform Ru nanoparticles, as confirmed by TEM images. The stronger interaction of Ru and cross‐shaped HZSM‐5 was simultaneously confirmed by a higher hydrogen reduction temperature of RuO2 on calcined Ru/HZSM‐5, a blueshift of Ruδ+‐(CO)n, Ruδ+‐(CO), and Ru0‐(CO) species in the IR spectra of adsorbed CO, and a higher Ru 3d5/2 binding energy in X‐ray photoelectron spectroscopy measurements. The reaction constant in guaiacol hydrogenolysis to phenol over cross‐shaped Ru/HZSM‐5 (0.051 min−1) was 3–4 times higher than that on spherical and cuboid Ru/HZSM‐5 (0.012–0.029 min−1) at identical conditions, attributed to the remarkable hydrogenolysis catalytic capability of Ru nanoparticles on cross‐shaped HZSM‐5. In addition, adsorption of guaiacol and hydrogen was more substantial on cross‐shaped Ru/HZSM‐5, as evidenced by of IR and mass spectroscopy, respectively. The higher adsorption of guaiacol is attributed to the abundant Lewis acid sites on cross‐shaped Ru/HZSM‐5, as the Al−OH enriched Lewis acid sites favor the adsorption of oxygen‐containing guaiacol. The higher rate constant in the primary step, together with the adsorbed high concentrated reactant and hydrogen (with nearly first‐order reaction kinetics) on cross‐shaped Ru/HZSM‐5, facilitates the overall tandem reaction, leading to an excellent hydrogenolysis catalyst working at hydrothermal conditions for biomass conversion.
A Novel Photo‐thermochemical Approach for Enhanced Carbon Dioxide Reforming of Methane
Authors: Pan Fuping , Xiang Xianmei , Deng Wei , Zhao Huilei , Feng Xuhui , Li Ying 
Published: November 2017

AbstractWe report a new approach for photo‐thermochemical CO2 (dry) reforming of methane (PTC‐DRM) to produce syngas by using concentrated sunlight as the energy input. A unique catalyst, Pt‐supported Si‐modified CeO2, was designed for this novel PTC‐DRM reaction by integrating photocatalytic and thermocatalytic effects, for which Pt was the thermocatalytic DRM catalyst and co‐catalyst for photocatalysis, CeO2 was the DRM support and semiconductor photocatalyst, and Si was the promoter and stabilizer. Under irradiation of the equivalent of 30 suns at 600 °C in 30 h, the production rates of H2 and CO reached stable levels of 90 and 154 mmol g−1 h−1, respectively, which were five and two times higher than those obtained in the dark at the same temperature. The significantly enhanced catalytic performance and stability under solar irradiation, resulting from synergy between the photocatalytic and thermocatalytic effects, demonstrates the feasibility of a new direction in low‐carbon fuel production from sunlight.