bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serotonin 5-HT1A receptor binding and self-transcendence in healthy control subjects - a replication study using Bayesian hypothesis testing
Authors: Griffioen Gina , Matheson Granville James , Cervenka Simon , Farde Lars , Borg Jacqueline 
Published: December 2017

AbstractObjectiveA putative relationship between markers for the serotonin system and the personality scale self-transcendence (ST) and its subscale spiritual acceptance (SA) has been demonstrated in a previous PET study of 5-HT1A receptor binding in healthy control subjects. The results could however not be replicated in a subsequent PET study at an independent centre. In this study, we performed a replication of our original study in a larger sample using Bayesian hypothesis testing to evaluate relative evidence both for and against this hypothesis.MethodsRegional 5-HT1A receptor binding potential (BPND) was examined in 50 healthy male subjects using PET with the radioligand [11C]WAY100635. 5-HT1A availability was calculated using the simplified reference tissue model (SRTM) yielding regional BPND. ST and SA were measured using the Temperament and Character Inventory (TCI) questionnaire. Correlations between ST/SA scores and 5-HT1A BPND in frontal cortex, hippocampus and raphe nuclei were examined by calculation of default correlation Bayes factors (BFs) and replication BFs.ResultsThere were no significant correlations between 5-HT1A receptor binding and ST/SA scores. Rather, five of six replication BFs provided moderate to strong evidence for no association between 5-HT1A availability and ST/SA, while the remaining BF provided only weak evidence.ConclusionWe could not replicate our previous findings of an association between 5-HT1A availability and the personality trait ST/SA. Rather, the Bayesian analysis provided evidence for a lack of correlation. Further research should focus on whether other components of the serotonin system may be related to ST or SA. This study also illustrates how Bayesian hypothesis testing allows for greater flexibility and more informative conclusions than traditional p-values, suggesting that this approach may be advantageous for analysis of molecular imaging data.
On the Impact of Interhemispheric White Matter: Age, Executive Functioning, and Dedifferentiation in the Frontal Lobes
Authors: Waters Abigail B. , Sawyer Kayle S. , Gansler David A. 
Published: December 2017

AbstractIntroductionIn middle age, declines in executive functioning (EF) are associated with decrements in the quality and/or quantity of white and grey matter. Recruitment of homologous regions has been identified as a compensatory mechanism for cognitive decline in later middle age, however research into neural substrates of EF has yet to be guided by dedifferentiation models. We hypothesized that frontal-parietal grey matter volume, interhemispheric white matter and intrahemispheric white matter fractional anisotropy (FA) will be predictive of EF. Further, we hypothesized that the comparative association between interhemispheric white matter and EF will increase with age, because of compensatory recruitment.MethodsNeurocognitive test data, DTI, and T1 MPRAGE scans (n= 444) were obtained from the NKI-Rockland Sample. Structural equation modeling was used to examine the relationship between age, EF, interhemispheric white matter (forceps minor; FM), intrahemispheric white matter (superior longitudinal fasciculus; SLF), and a frontal-parietal grey matter network. EF and grey matter were modelled as latent variables, with EF examined as the criterion. Additionally, a subsample of participants aged 55-85 (n= 168) was analyzed to examine the influence of age related compensatory mechanisms.ResultsThere was a significant relationship between FM, grey matter, and EF, which was fully mediated by age. There was a significant relationship between SLF and EF, which was not mediated by age. For older adults, only the age-mediated pathway from FM to EF was significant.DiscussionUsing structural imaging data, support was found for age-related interhemispheric mechanisms of compensation, but not intrahemispheric mechanisms.Key points(1) Neural substrates of executive functioning are not static across the lifespan. (2) In older adults, white matter becomes more salient as a structural correlate of executive functioning, as recruitment needs increase. (3) While the importance of interhemispheric white matter is mediated by age, intrahemispheric recruitment remains consistent across the lifespan, and is not the primary mechanism of age-based compensation in community dwelling older adults.
Visual Representation of Experimental Protocols
Authors: Scott-Brown James , Papachristodoulou Antonis 
Published: December 2017

AbstractUsing robots to automate laboratory tasks could increase throughput and reproducibility, but requires experimental protocols to be specified in a computer-readable format. We present a new user interface (Lists of Liquids) for specifying experimental protocols by directly manipulating a diagram: rather than having to specify individual liquid handling operations, the user can simply specify that particular lists of liquids should be combined as either a Cartesian product or convolution, and the system will plan a series of liquid handling steps to achieve this. This is intended to provide a higher-level interface in order to make the creation of protocols faster and less error prone.
Catalytic potential and disturbance rejection of glycolytic kinases in the human red blood cell
Authors: Yurkovich James T. , Alcantar Miguel A. , Haiman Zachary B. , Palsson Bernhard O. 
Published: December 2017

AbstractThe allosteric regulation of metabolic enzymes plays a key role in controlling the flux through metabolic pathways. The activity of such enzymes is traditionally described by allosteric rate laws in complex kinetic models of metabolic network function. As an alternative, we describe the fraction of the regulated enzyme that is in an active form by developing a detailed reaction network of all known ligand binding events to the enzyme. This fraction is the fundamental result of metabolic regulation as it represents the “tug of war” among the various regulators and substrates that determine the utilization of the enzyme. The active fraction corresponds to the utilization of the catalytic potential of the enzyme. Using well developed kinetic models of human red blood cell (RBC) glycolysis, we characterize the catalytic potential of its three key kinases: hexokinase (HEX), phosphofructokinase (PFK), and pyruvate kinase (PYK). We then compute their time-dependent interacting catalytic potentials. We show how detailed kinetic models of the management of the catalytic potential of the three kinases elucidates disturbance rejection capabilities of glycolysis. Further, we examine the sensitivity of the catalytic potential through an examination of existing personalized RBC models, providing a physiologically-meaningful sampling of the feasible parameter space. The graphical representation of the dynamic interactions of the individual kinase catalytic potential adjustment provides an easy way to understand how a robust homeostatic state is maintained through interacting allosteric regulatory mechanisms.
Assessment of the performance of different hidden Markov models for imputation in animal breeding
Authors: Whalen Andrew , Gorjanc Gregor , Ros-Freixedes Roger , Hickey John M 
Published: December 2017

AbstractIn this paper we review the performance of various hidden Markov model-based imputation methods in animal breeding populations. Traditionally, heuristic-based imputation methods have been used for imputation in large animal populations due to their computational efficiency, scalability, and accuracy. However, recent advances in the area of human genetics have increased the ability of probabilistic hidden Markov model methods to perform accurate phasing and imputation in large populations. These advances may enable these methods to be useful for routine use in large animal populations. To test this, we evaluate here the accuracy and computational cost of several methods in a series of simulated populations and a real animal population. We first tested single-step (diploid) imputation, which performs both phasing and imputation. Then we tested pre-phasing followed by haploid imputation. We tested four diploid imputation methods (fastPHASE, Beagle v4.0, IMPUTE2, and MaCH), three phasing methods, (SHAPEIT2, HAPI-UR, and Eagle2), and three haploid imputation methods (IMPUTE2, Beagle v4.1, and minimac3). We found that performing pre-phasing and haploid imputation was faster and more accurate than diploid imputation. In particular, we found that pre-phasing with Eagle2 or HAPI-UR and imputing with minimac3 or IMPUTE2 gave the highest accuracies in both simulated and real data.
No clear evidence for correlations between handgrip strength and sexually dimorphic acoustic properties of voices
Authors: Han Chengyang , Wang Hongyi , Fasolt Vanessa , Hahn Amanda C , Holzleitner Iris J , Lao Junpeng , DeBruine Lisa M , Feinberg David R , Jones Benedict C 
Published: December 2017

AbstractObjectivesRecent research on the signal value of masculine physical characteristics in men has focused on the possibility that such characteristics are valid cues of physical strength. However, evidence that sexually dimorphic vocal characteristics are correlated with physical strength is equivocal. Consequently, we undertook a further test for possible relationships between physical strength and masculine vocal characteristics.MethodsWe tested the putative relationships between White UK (N=115) and Chinese (N=106) participants’ handgrip strength (a widely used proxy for general upper-body strength) and five sexually dimorphic acoustic properties of voices: fundamental frequency (F0), fundamental frequency’s standard deviation (F0-SD), formant dispersion (Df), formant position (Pf), and estimated vocal-tract length (VTL).ResultsAnalyses revealed no clear evidence that stronger individuals had more masculine voices.ConclusionsOur results do not support the hypothesis that masculine vocal characteristics are a valid cue of physical strength.
Characterisation of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum
Authors: Hulin M.T. , Mansfield J.W. , Brain P. , Xiangming X. , Jackson R.W. , Harrison R.J. 
Published: December 2017

AbstractBacterial canker is a major disease of cherry and other stone fruits caused by several pathovars of Pseudomonas syringae. These are P.s pv. morsprunorum race 1 (Psm R1), P.s pv. morsprunorum race 2 (Psm R2) and P.s pv. syringae (Pss). Psm R1 and R2 were originally designated as races of the same pathovar, however phylogenetic analysis has revealed them to be distantly related. This study characterised the pathogenicity of P. syringae on cherry and plum, in the field and the laboratory. The field experiment identified variation in host cultivar susceptibility to the different pathogen clades. The cherry cultivar Merton Glory exhibited a broad resistance to all clades, whilst cultivar Van showed race-specific resistance. Psm R1 may be divided into a race structure with some strains pathogenic to both cherry and plum and others only pathogenic to plum. The results of laboratory-based pathogenicity tests were compared to results obtained on whole-trees. Only cut shoot inoculations were found to be sensitive enough to detect cultivar variation in susceptibility. Measuring population growth of bacteria in detached leaves reliably discriminated pathogens from non-pathogens. In addition, symptom appearance discriminated Psm races from non-pathogens which triggered a rapid hypersensitive response (HR). The pathogen Pss rapidly induced disease lesions and therefore may exhibit a more necrotrophic lifestyle than hemi-biotrophic Psm races. This in-depth study of pathogenic interactions, identification of host resistance and optimisation of laboratory assays, will provide a framework for future genetic dissection of virulence and host resistance mechanisms.
Gene regulatory network rewiring by an adaptively evolving microRNA cluster inDrosophila
Authors: Lyu Yang , Liufu Zhongqi , Xiao Juan , Chen Yuxin , Wu Chung-I , Tang Tian 
Published: December 2017

AbstractNew miRNAs are evolutionarily important but their impact on existing biological networks remains unclear. We report the evolution of a microRNA cluster,mir-972C, that arosede novoand the subsequently rewired gene regulatory networks inDrosophila. Molecular evolution analyses revealed thatmir-972Coriginated in the common ancestor ofDrosophilawhere it comprises five old miRNAs. It subsequently recruited five new members in themelanogastersubgroup after conservative evolution for at least 50 million years. Population genetics analyses reveal that young and oldmir-972CmiRNAs evolved rapidly under positive selection in both seed and non-seed regions. Combining target prediction and cell transfection experiments, we find that sequence changes in individualmir-972Cmembers resulted in extensive gene regulatory network divergence amongD. melanogaster, D. simulans, andD. virilis, whereas the target pool of the cluster as a whole remains relatively conserved. Our results suggest that clustering of young and old miRNAs at the same locus broadens target repertoires, resulting in the gain of new targets without losing many old ones. This may facilitate the establishment of new miRNAs within existing regulatory networks.
Counterfactual reasoning underlies the learning of priors in decision making
Authors: Zylberberg Ariel , Wolpert Daniel M , Shadlen Michael N 
Published: December 2017

SummaryAccurate decisions require knowledge of prior probabilities (e.g., prevalence or base rate) but it is unclear how prior probability is learned in the absence of a teacher. We hypothesized that humans could learn base rates from experience making decisions, even without feedback. Participants made difficult decisions about the direction of dynamic random dot motion. For each block of 15-42 trials, the base rate favored left or right by a different amount. Participants were not informed of the base rate, yet they gradually biased their choices and thereby increased accuracy and confidence in their decisions. They achieved this by updating knowledge of base rate after each decision, using a counterfactual representation of confidence that simulates a neutral prior. The strategy is consistent with Bayesian updating of belief and suggests that humans represent both true confidence, that incorporates the evolving belief of the prior, and counterfactual confidence that discounts the prior.
Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization
Authors: Jack Brittany , Mueller David M. , Fee Ann C. , Tetlow Ashley , Avasthi Prachee 
Published: December 2017

ABSTRACTFlagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of mammalian cells and provide an excellent model to study ciliogenesis. These biflagellated cells have two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Previously, we described a role for actin in the regulation of flagella-building intraflagellar transport machinery. Here, we probe how actin redundancy contributes to this process using a nap1 mutant Chlamydomonas strain. Disruption of a single actin allows normal or slower incorporation but complete flagellar assembly. However, when we disrupt both actins using Latrunculin B (LatB) treatment on the nap1 mutant background, we find flagellar growth from newly synthesized limiting flagellar proteins is actin-dependent. Upon total actin disruption during flagellar assembly, transmission electron microscopy identified an accumulation of Golgi-adjacent vesicles, suggesting impaired vesicular trafficking may be the mechanism by which actin supports flagellar growth from new flagellar proteins. We also find there is a mislocalization of a key transition zone gating and ciliopathy protein, NPHP-4. Extended (2 hour) treatment with LatB, a condition under which NAP1 is upregulated, restores NPHP-4 localization. This suggests NAP1 can perform the functions of conventional actin at the transition zone. Our experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for these actins in transport of Golgi-adjacent vesicles and flagellar incorporation of newly synthesized proteins.
Modeling bid/no bid decisions using fuzzy fault tree
Authors: Marzouk Mohamed , Mohamed Emad 
Published: December 2017

Purpose
Decisions by construction contractors to bid (or not to bid) require the thorough assessment and evaluation of factors relevant to the decision, as well as the quantification of their combined impact, to produce successful bid/no-bid decisions. The purpose of this study is to present a fuzzy fault tree model to assist construction contractors to more efficiently bid for future projects.

Design/methodology/Approach
The proposed model consist of two stages: first, identification of the factors that affect bidding decision using a questionnaire survey after an extensive literature review, and second, usage of the identified factors to build a fuzzy fault tree model to simulate the bidding decision.

Findings
A list of 15 factors that affect bid/no-bid decisions was identified. Analysis of factors revealed that the highest-ranking factors were related to financial aspects of the project. A case study is presented to demonstrate the capabilities of the model, and a fuzzy important analysis is performed on the basic events to demonstrate the differences between three contractors’ bid/no-bid decisions. The results reveal that there is variation between the decisions of each contractor based on their willingness to participate. Besides, the influence of evaluation factors on the final decision for each contractor is different.

Originality/value
The study contributes to the body of knowledge on tendering and bidding practices. The proposed model incorporated the fuzzy set theory, which suits human subjectivity. The proposed methodology overcomes the limitations of previous models as it can, using the linear pool opinion principle, combine and weigh the evaluations of multiple experts. In addition, the model is convenient for situations where historical data are not available.
The impact of social influence on the relationship between personality traits and perceived investment performance of individual investors
Authors: Akhtar Fatima , Thyagaraj K.S. , Das Niladri 
Published: December 2017

PurposeThe purpose of this paper is to clarify the relationship between an individual investor’s personality trait and his perceived investment performance. It proposes a novel conceptual framework that integrates social influence (as a moderating construct) and outlines the role of personality in determining the perceived investment performance during the investment decision-making process.Design/methodology/approachA questionnaire-based survey was conducted to collect responses from 396 individual investors through stratified and quota sampling approach. The collected data were then analysed using both hierarchical regression analysis and structural equation modelling to evaluate the strength of the relationship between the constructs, namely, personality trait, perceived investment performance and social influence.FindingsThis study suggests that social influence positively moderates the relationship between extraversion-perceived investment performance, whereas it negatively moderates the relationship between agreeability-perceived investment performance.Research limitations/implicationsThis study has certain limitations. First, this work follows a modelling approach which is more centred towards the prediction of relationships. Second, because of choosing a research approach (since the study has been conducted in one country, i.e. India), the results of the study may lack generalisability. Therefore, further studies could be encouraged to test the proposed hypotheses.Practical implicationsInsights from this study suggest that investors should look in for their personality traits while making an investment decision. In fact, psychologically modified portfolios should be developed as per the personality traits of the investors.Originality/valueThe study, perhaps, is the only study to apply social influence in a framework using Big Five personality traits as a possible factor to understand the individual differences in terms of perceived investment performance.
The production and use of indicators in science, technology and innovation policy-making in Africa
Authors: Manyuchi Albert Edgar , Mugabe John Ouma 
Published: December 2017

Purpose
A growing number of African countries are starting to produce science, technology and innovation (STI) indicators. The purpose of this paper is to provide some lessons learnt in the production and use of STI indicators in Malawi and South Africa. It is compares the two countries’ efforts to conduct Research and Development (R&D) surveys and examines whether and how STI indicators are used in policymaking processes.

Design/methodology/approach
The study approach is qualitative. The research methodology encompasses a thorough review of both policy and academic literature as well as some interviews.

Findings
The study demonstrates that South Africa has a relatively developed institutional arrangement for undertaking R&D and innovation surveys and developing related STI indicators. There is evidence that efforts are being made to use STI indicators to inform policymaking in the country. On the other hand, Malawi conducted its first R&D survey under the African Science, Technology and Innovation Indicators Initiative (ASTII) and has not established an institutional mechanism dedicated to producing STI indicators. There is no evidence that indicators are used in, or to inform, policymaking in the country.

Research limitations/implications
Because of significant differences in STI policymaking histories, capacities and cultures of the two countries, it is not really useful to compare the STI production and use. Rather it is important to draw lessons from the efforts of the two countries.

Practical implications
The results suggest that the production of STI indicators should be embedded in policy processes. To be useful and effective, STI indicators production needs to be explicitly linked to policy formulation, evaluation and monitoring activities without necessarily undermining the independence of producing STI indicators.

Social implications
Creating stand-alone programmes or agencies for R&D and innovation surveys without clear articulation with policymaking needs erodes opportunities of having evidence-based STI policy regimes.

Originality/value
Although in 2005 only South Africa and Tunisia had national programmes dedicated to the generation of R&D statistics, by the end of 2010 at least 19 African countries had experimented with conducting R&D surveys under the auspices of the ASTII of the New Partnership for Africa’s Development. These countries accumulated different experiences and consequently build different kinds of institutional capacities. Through the Malawi and South Africa case studies, some important lessons for STI indicators production and use and STI policymaking can be drawn for developing countries in general and African countries in particular.