bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microglia retard dengue virus-induced acute viral encephalitis
Authors: Tsai Tsung-Ting , Chen Chia-Ling , Lin Yee-Shin , Chang Chih-Peng , Tsai Cheng-Chieh , Cheng Yi-Lin , Huang Chao-Ching , Ho Chien-Jung , Lee Yi-Chao , Lin Liang-Tzung , Jhan Ming-Kai , Lin Chiou-Feng 
Published: June 2016

AbstractPatients with dengue virus (DENV) infection may also present acute viral encephalitis through an unknown mechanism. Here, we report that encephalitic DENV-infected mice exhibited progressive hunchback posture, limbic seizures, limbic weakness, paralysis and lethality 7 days post-infection. These symptoms were accompanied by CNS inflammation, neurotoxicity and blood-brain barrier destruction. Microglial cells surrounding the blood vessels and injured hippocampus regions were activated by DENV infection. Pharmacologically depleting microglia unexpectedly increased viral replication, neuropathy and mortality in DENV-infected mice. In microglia-depleted mice, the DENV infection-mediated expression of antiviral cytokines and the infiltration of CD8-positive cytotoxic T lymphocytes (CTLs) was abolished. DENV infection prompted the antigen-presenting cell-like differentiation of microglia, which in turn stimulated CTL proliferation and activation. These results suggest that microglial cells play a key role in facilitating antiviral immune responses against DENV infection and acute viral encephalitis.
Active dynamics of colloidal particles in time-varying laser speckle patterns
Authors: Bianchi Silvio , Pruner Riccardo , Vizsnyiczai Gaszton , Maggi Claudio , Di Leonardo Roberto 
Published: June 2016

AbstractColloidal particles immersed in a dynamic speckle pattern experience an optical force that fluctuates both in space and time. The resulting dynamics presents many interesting analogies with a broad class of non-equilibrium systems like: active colloids, self propelled microorganisms, transport in dynamical intracellular environments. Here we show that the use of a spatial light modulator allows to generate light fields that fluctuate with controllable space and time correlations and a prescribed average intensity profile. In particular we generate ring-shaped random patterns that can confine a colloidal particle over a quasi one-dimensional random energy landscape. We find a mean square displacement that is diffusive at both short and long times, while a superdiffusive or subdiffusive behavior is observed at intermediate times depending on the value of the speckles correlation time. We propose two alternative models for the mean square displacement in the two limiting cases of a short or long speckles correlation time. A simple interpolation formula is shown to account for the full phenomenology observed in the mean square displacement across the entire range from fast to slow fluctuating speckles.
Nodal marginal zone B cells in mice: a novel subset with dormant self-reactivity
Authors: Palm Anna-Karin E. , Friedrich Heike C. , Kleinau Sandra 
Published: June 2016

AbstractMarginal zone (MZ) B cells, representing a distinct subset of innate-like B cells, mount rapid T-independent responses to blood-borne antigens. They express low-affinity polyreactive antigen receptors that recognize both foreign and self-structures. The spleen is considered the exclusive site for murine MZ B cells. However, we have here identified B cells with a MZ B-cell phenotype in the subcapsular sinuses of mouse lymph nodes. The nodal MZ (nMZ) B cells display high levels of IgM, costimulators and TLRs, and are represented by naïve and memory cells. The frequency of nMZ B cells is about 1–6% of nodal B cells depending on mouse strain, with higher numbers in older mice and a trend of increased numbers in females. There is a significant expansion of nMZ B cells following immunization with an autoantigen, but not after likewise immunization with a control protein or with the adjuvant alone. The nMZ B cells secrete autoantibodies upon activation and can efficiently present autoantigen to cognate T cells in vitro, inducing T-cell proliferation. The existence of self-reactive MZ B cells in lymph nodes may be a source of autoantigen-presenting cells that in an unfortunate environment may activate T cells leading to autoimmunity.
Stimulus-Dependent Inhibitor of Apoptosis Protein Expression Prolongs the Duration of B Cell Signalling
Authors: Shinohara Hisaaki , Inoue Kentaro , Yumoto Noriko , Nagashima Takeshi , Okada-Hatakeyama Mariko 
Published: June 2016

AbstractDifferent dynamic behaviours of signalling activity can induce distinct biological responses in a variety of cells. However, the molecular mechanisms that determine the dynamics of kinase activities in immune cells are not well understood. In this study, we showed that the duration of both IκB kinase (IKK) and extracellular signal-regulated kinase (ERK) activities in B cell receptor (BCR)- and CD40-signalling pathways in B cells were regulated by transcriptional feedback loops. We conducted a time-course transcriptome analysis after BCR or CD40 stimulation and identified the following four candidate genes as feedback regulators for IKK and ERK: inhibitor of apoptosis protein (IAP), TNF alpha-induced protein 3, dual-specificity phosphatase 5 and sprouty homolog 2. Quantitative experiments and mathematical modelling suggested that IAP inhibition shortened the duration of IKK and ERK activity following both BCR and CD40 pathway stimulation, indicating a positive role for IAP in B cell signalling. Furthermore, transient kinase activities induced by IAP blockage reduced the levels of delayed expression genes. Together, our findings suggest that IKK and ERK activity durations can be fine-tuned by the coordinated regulation of positive and negative transcriptional feedback and that these network properties determine the biological output of B cells.
Genomic leftovers: identifying novel microsatellites, over-represented motifs and functional elements in the human genome
Authors: Fonville Natalie C. , Velmurugan Karthik Raja , Tae Hongseok , Vaksman Zalman , McIver Lauren J. , Garner Harold R. 
Published: June 2016

AbstractThe human genome is 99% complete. This study contributes to filling the 1% gap by enriching previously unknown repeat regions called microsatellites (MST). We devised a Global MST Enrichment (GME) kit to enrich and nextgen sequence 2 colorectal cell lines and 16 normal human samples to illustrate its utility in identifying contigs from reads that do not map to the genome reference. The analysis of these samples yielded 790 novel extra-referential concordant contigs that are observed in more than one sample. We searched for evidence of functional elements in the concordant contigs in two ways: (1) BLAST-ing each contig against normal RNA-Seq samples, (2) Checking for predicted functional elements using GlimmerHMM. Of the 790 concordant contigs, 37 had an exact match to at least one RNA-Seq read; 15 aligned to more than 100 RNA-Seq reads. Of the 249 concordant contigs predicted by GlimmerHMM to have functional elements, 6 had at least one exact RNA-Seq match. BLAST-ing these novel contigs against all publically available sequences confirmed that they were found in human and chimpanzee BAC and FOSMID clones sequenced as part of the original human genome project. These extra-referential contigs predominantly contained pentameric repeats, especially two motifs: AATGG and GTGGA.
Highly selective and sensitive phosphate anion sensors based on AlGaN/GaN high electron mobility transistors functionalized by ion imprinted polymer
Authors: Jia Xiuling , Chen Dunjun , Bin Liu , Lu Hai , Zhang Rong , Zheng Youdou 
Published: June 2016

AbstractA novel ion-imprinted electrochemical sensor based on AlGaN/GaN high electron mobility transistors (HEMTs) was developed to detect trace amounts of phosphate anion. This sensor combined the advantages of the ion sensitivity of AlGaN/GaN HEMTs and specific recognition of ion imprinted polymers. The current response showed that the fabricated sensor is highly sensitive and selective to phosphate anions. The current change exhibited approximate linear dependence for phosphate concentration from 0.02 mg L−1 to 2 mg L−1, the sensitivity and detection limit of the sensor is 3.191 μA/mg L−1 and 1.97 μg L−1, respectively. The results indicated that this AlGaN/GaN HEMT-based electrochemical sensor has the potential applications on phosphate anion detection.
Stable and null current hysteresis perovskite solar cells based nitrogen doped graphene oxide nanoribbons hole transport layer
Authors: Kim Jeongmo , Mat Teridi Mohd Asri , Mohd Yusoff Abd. Rashid bin , Jang Jin 
Published: June 2016

AbstractPerovskite solar cells are becoming one of the leading technologies to reduce our dependency on traditional power sources. However, the frequently used component poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) has several shortcomings, such as an easily corroded indium-tin-oxide (ITO) interface at elevated temperatures and induced electrical inhomogeneity. Herein, we propose solution-processed nitrogen-doped graphene oxide nanoribbons (NGONRs) as a hole transport layer (HTL) in perovskite solar cells, replacing the conducting polymer PEDOT:PSS. The conversion efficiency of NGONR-based perovskite solar cells has outperformed a control device constructed using PEDOT:PSS. Moreover, our proposed NGONR-based devices also demonstrate a negligible current hysteresis along with improved stability. This work provides an effective route for substituting PEDOT:PSS as the effective HTL.
Synthesis of chemically modified DNA
Authors: Shivalingam Arun , Brown Tom 
Published: June 2016

Naturally occurring DNA is encoded by the four nucleobases adenine, cytosine, guanine and thymine. Yet minor chemical modifications to these bases, such as methylation, can significantly alter DNA function, and more drastic changes, such as replacement with unnatural base pairs, could expand its function. In order to realize the full potential of DNA in therapeutic and synthetic biology applications, our ability to ‘write’ long modified DNA in a controlled manner must be improved. This review highlights methods currently used for the synthesis of moderately long chemically modified nucleic acids (up to 1000 bp), their limitations and areas for future expansion.
Aftershock seismicity and tectonic setting of the 2015 September 16 Mw 8.3 Illapel earthquake, Central Chile
Authors: Lange Dietrich , Geersen Jacob , Barrientos Sergio , Moreno Marcos , Grevemeyer Ingo , Contreras-Reyes Eduardo , Kopp Heidrun 
Published: June 2016

Abstract
Powerful subduction zone earthquakes rupture thousands of square kilometres along continental margins but at certain locations earthquake rupture terminates. To date, detailed knowledge of the parameters that govern seismic rupture and aftershocks is still incomplete. On 2015 September 16, the Mw 8.3 Illapel earthquake ruptured a 200 km long stretch of the Central Chilean subduction zone, triggering a tsunami and causing significant damage. Here, we analyse the temporal and spatial pattern of the coseismic rupture and aftershocks in relation to the tectonic setting in the earthquake area. Aftershocks cluster around the area of maximum coseismic slip, in particular in lateral and downdip direction. During the first 24 hr after the main shock, aftershocks migrated in both lateral directions with velocities of approximately 2.5 and 5 km hr−1. At the southern rupture boundary, aftershocks cluster around individual subducted seamounts that are related to the downthrusting Juan Fernández Ridge. In the northern part of the rupture area, aftershocks separate into an upper cluster (above 25 km depth) and a lower cluster (below 35 km depth). This dual seismic–aseismic transition in downdip direction is also observed in the interseismic period suggesting that it may represent a persistent feature for the Central Chilean subduction zone.
Reported Distribution of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus in the United States, 1995-2016 (Diptera: Culicidae)
Authors: Hahn Micah B , Eisen Rebecca J , Eisen Lars , Boegler Karen A , Moore Chester G , McAllister Janet , Savage Harry M , Mutebi John-Paul 
Published: June 2016

Abstract
Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus (Skuse) transmit arboviruses that are increasing threats to human health in the Americas, particularly dengue, chikungunya, and Zika viruses. Epidemics of the associated arboviral diseases have been limited to South and Central America, Mexico, and the Caribbean in the Western Hemisphere, with only minor localized outbreaks in the United States. Nevertheless, accurate and up-to-date information for the geographical ranges of Ae. aegypti and Ae. albopictus in the United States is urgently needed to guide surveillance and enhance control capacity for these mosquitoes. We compiled county records for presence of Ae. aegypti and Ae. albopictus in the United States from 1995-2016, presented here in map format. Records were derived from the Centers for Disease Control and Prevention ArboNET database, VectorMap, the published literature, and a survey of mosquito control agencies, university researchers, and state and local health departments. Between January 1995 and March 2016, 183 counties from 26 states and the District of Columbia reported occurrence of Ae. aegypti, and 1,241 counties from 40 states and the District of Columbia reported occurrence of Ae. albopictus. During the same time period, Ae. aegypti was collected in 3 or more years from 94 counties from 14 states and the District of Columbia, and Ae. albopictus was collected during 3 or more years from 514 counties in 34 states and the District of Columbia. Our findings underscore the need for systematic surveillance of Ae. aegypti and Ae. albopictus in the United States and delineate areas with risk for the transmission of these introduced arboviruses.
Bevacizumab in high-grade glioma patients following intraparenchymal hemorrhage
Authors: Lin Xuling , Daras Mariza , Pentsova Elena , Nolan Craig P. , Gavrilovic Igor T. , DeAngelis Lisa M. , Kaley Thomas J. 
Published: June 2016

AbstractBackgroundIntraparenchymal hemorrhage (IPH) is a relative contraindication to bevacizumab therapy, an anti-vascular endothelial growth factor (VEGF) monoclonal antibody approved for the treatment of recurrent glioblastoma. However, in patients with symptomatic enhancing tumors and poor functional status, bevacizumab may be the only beneficial therapeutic option.MethodsWe retrospectively reviewed all patients with high-grade glioma who were treated between January 1, 2005 and December 31, 2014 with bevacizumab despite prior IPH.ResultsEighteen patients met our study criteria. There were 12 women and 6 men with a median age of 56 years. Tumor types were glioblastoma (n = 15), anaplastic astrocytoma (n = 2), and anaplastic oligodendroglioma (n = 1). Seventeen patients had prior spontaneous intratumoral bleed (13 grade 1–2; 4 grade 3–4); the 1 remaining patient had a grade 3 bleed due to cerebral venous thrombosis. Among them, identifiable risk factors for hemorrhage were anti-VEGF therapy, anticoagulation use, thrombocytopenia, and hypertension; seven had no identifiable risk factors. The median duration from IPH to (re-)initiation of bevacizumab was 113 days (range 13–1367). Brain imaging performed prior to bevacizumab treatment showed persistent or evolving hemorrhage in 8 patients and complete resolution in 10 patients. With a median follow-up duration of 137 days after bevacizumab re-initiation, only 1 (6%) of the 18 patients re-bled; this patient had an anaplastic oligodendroglioma and developed a grade 2 intratumoral bleed after 3 doses of bevacizumab.ConclusionsThe incidence of re-bleed is rare. Bevacizumab use was safe in patients with recurrent high-grade glioma following IPH for whom no other meaningful treatment options existed.
Mixed convection squeezing three-dimensional flow in a rotating channel filled with nanofluid
Authors: Mahanthesh B , Gireesha B J , Gorla R S R 
Published: June 2016

Purpose
– The purpose of this paper is to numerically solve the problem of an unsteady squeezing three-dimensional flow and heat transfer of a nanofluid in rotating vertical channel of stretching left plane. The fluid is assumed to be Newtonian, incompressible and electrically conducting embedded with nanoparticles. Effect of internal heat generation/ absorption is also considered in energy equation. Four different types of nanoparticles are considered, namely, copper (Cu), alumina (Al2O3), silver (Ag) and titanium oxide (TiO2) with the base fluid as water. Maxwell-Garnetts and Brinkman models are, respectively, employed to calculate the effective thermal conductivity and viscosity of the nanofluid.

Design/methodology/approach
– Using suitable similarity transformations, the governing partial differential equations are transformed into set of ordinary differential equations. Resultant equations have been solved numerically using Runge-Kutta-Fehlberg fourth fifth order method for different values of the governing parameters. Effects of pertinent parameters on normal, axial and tangential components of velocity and temperature distributions are presented through graphs and discussed in detail. Further, effects of nanoparticle volume fraction, squeezing parameter, suction/injection parameter and heat source/sink parameter on skin friction and local Nusselt number profiles for different nanoparticles are presented in tables and analyzed.

Findings
– Squeezing effect enhances the temperature field and consequently reduces the heat transfer rate. Large values of mixed convection parameter showed a significant effect on velocity components. Also, in many heat transfer applications, nanofluids are potentially useful because of their novel properties. They exhibit high-thermal conductivity compared to the base fluids. Further, squeezing and rotation effects are desirable in control the heat transfer.

Originality/value
– Three-dimensional mixed convection flows over in rotating vertical channel filled with nanofluid are very rare in the literature. Mixed convection squeezing three-dimensional flow in a rotating channel filled with nanofluid is first time investigated.
Effects of magnetic field and viscous dissipation on entropy generation of mixed convection in porous lid-driven cavity with corner heater
Authors: Ahmed Sameh E , Öztop Hakan F. , Al-Salem Khaled 
Published: June 2016

Purpose
– The purpose of this paper is to investigate the effects of magnetic field and viscous dissipation on mixed convection heat transfer, fluid flow and entropy generation in a porous media filled square enclosure heated with corner isothermal heater.

Design/methodology/approach
– Finite volume method has been used to solve governing equations. A code is developed by FORTRAN and entropy generation is calculated from the obtained results of velocities and temperature. Results are presented via streamlines, isotherms, local and mean Nusselt number for different values of Richardson number (0.001=Ri=100), Hartmann number (0.001=Ha=100), Darcy number (0.001=Da=0.1), length of heaters (0.25=hx=hy=0.75) and viscous dissipation factors (10−4=ε=10−6).

Findings
– It is observed that entropy is generated mostly due to lid-driven wall and right side of the heater. Entropy generation decreases with increasing of Hartmann number and heat transfer increases with decreasing of viscous parameter.

Originality/value
– The originality of this work is to application of magnetic field and viscous dissipation on entropy generation in a lid-driven cavity with corner heater. Here, both corner heater and the external forces are original parameters.
Integrating unorganized volunteers in emergency response management
Authors: Skar Marit , Sydnes Maria , Sydnes Are Kristoffer 
Published: June 2016

Purpose– When emergencies occur, ordinary members of the public are often the first to respond. However, their use and integration in emergency response remain a challenge. The purpose of this paper is to explore mechanisms and strategies for integrating unorganized volunteers in emergency response.Design/methodology/approach– This is a qualitative case study. A series of anonymized, semi-structured interviews were conducted with representatives of the key emergency response organizations – the police, ambulance service, fire and rescue service, and the Red Cross, located in the city of Tromsø. In addition, regulatory documents used by these organizations were examined, including laws, contingency plans, procedural handbooks and checklists.Findings– Professional responders acknowledge the resource unorganized volunteers may represent when additional capacity is needed. However, being uncertain about their availability and competence, professional responders find it hard to integrate unorganized volunteers through formal mechanisms as contingency planning and exercises, but rather rely on informal and individual case-by-case considerations. The local Red Cross, who are part of the established response system, are developing procedures to integrate unorganized volunteers through training, exercises and response operations. This provides an innovative hybrid approach to volunteer management.Originality/value– Available research provides limited information and advice on how to integrate unorganized volunteers effectively in emergencies. This study provides insights in formal and informal mechanisms of integrating unorganized volunteers in emergency response. It also provides lessons from a case of volunteer management through the Red Cross.
London’s burning: integrating water flow rates and building types into fire risk maps
Authors: Ronan Thomas , Teeuw Richard 
Published: June 2016

Purpose
– The purpose of this paper is to explore fire risk and preparedness, with regard to water flow rates and building types in London, focusing on Southall district.

Design/methodology/approach
– A land use survey was carried out to identify water requirements for firefighting across the study area. Local fire hydrant flow rates were analysed, using measurements taken during 2013 and archive data held by the London Fire Brigade (LFB). QGIS was used to explore relationships between fire hydrant flow rates, urban fire risk and socio-economic vulnerability data held by the LFB.

Findings
– A new type of map, which includes data on water flow rates and building types, was created using QGIS and applied to Southall district, resulting in a map showing Combined Vulnerability to fires. Inadequate fire hydrant water supply was found across many parts of the borough.

Practical implications
– This new approach to the evaluation and mapping of urban fire risk could be applied in other cities, to assess problems with water supply and the firefighting water flow requirements of various building types. The methodology can thus assist with adaptations to urban fire resource allocation, tactics, planning and preparedness.

Social implications
– When socio-economic data are also available, this Geographical Information System-based methodology becomes very useful for assessing fire risk and developing strategies for preparedness and response.

Originality/value
– This is the first time that London’s fire hydrant water pressures have been mapped and linked with socio-economic vulnerability maps, to produce a Combined Vulnerability map for assessing fire risk.