bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbon Dots-AS1411 Aptamer Nanoconjugate for Ultrasensitive Spectrofluorometric Detection of Cancer Cells
Authors: Motaghi Hasan , Mehrgardi Masoud Ayatollahi , Bouvet Philippe 
Published: August 2017

AbstractIn the present study, a sensitive and selective signal-on method for aptamer based spectrofluorometric detection of cancer cells is introduced. AS1411, a nucleolin aptamer, is wrapped around water-soluble carbon dots and used as a probe for the detection of several types of cancer cells. Nucleolin, is overexpressed on the surface of cancer cells. Mouse breast 4T1, human breast MCF7, and human cervical HeLa cancer cells were selected as target cells, while human foreskin fibroblast cells HFFF-PI6 served as control cells. For the sensitive and selective spectrofluorimetric detection of target cancer cells in the presence of control cells, the cells were incubated in carbon dots-aptamer solutions, the cell suspensions were subsequently centrifuged and the fluorescence intensities were measured as an analytical signal. The specific targeting of cancer cells by AS1411 aptamers causes the release of carbon dots and enhances the fluorescence intensity. A calibration curve with a dynamic range between 10–4500 4T1 cells and detectability of roughly 7 cells was obtained. In addition, no significant change in the signal was detected by modifying the amount of human foreskin fibroblast control cells. Our results demonstrate similar responses to human MCF7 breast and cervical HeLa cancer cells.
A new red cell index and portable RBC analyzer for screening of iron deficiency and Thalassemia minor in a Chinese population
Authors: Tong Lieshu , Kauer Josef , Wachsmann-Hogiu Sebastian , Chu Kaiqin , Dou Hu , Smith Zachary J. 
Published: August 2017

AbstractAnemia is a widespread public health problem with 1/4 ~1/3 of the world’s population being affected. In Southeast Asia, Thalassemia trait (TT) and iron deficiency anemia (IDA) are the two most common anemia types and can have a serious impact on quality of life. IDA patients can be treated with iron supplementation, yet TT patients have diminished capacity to process iron. Therefore, distinguishing between types of anemia is essential for effective diagnosis and treatment. Here, we present two advances towards low-cost screening for anemia. First: a new red-cell-based index, Joint Indicator A, to discriminate between IDA, TT, and healthy children in a Chinese population. We collected retrospective data from 384 Chinese children and used discriminant function analysis to determine the best analytic function to separate healthy and diseased groups, achieving 94% sensitivity and 90% specificity, significantly higher than reported indices. This result is achieved using only three red cell parameters: mean cell volume (MCV), red cell distribution width (RDW) and mean cell hemoglobin concentration (MCHC). Our second advance: the development of a low cost, portable red cell analyzer to measure these parameters. Taken together, these two results may help pave the way for widespread screening for nutritional and genetic anemias.
Monitoring Pertussis Infections Using Internet Search Queries
Authors: Zhang Yuzhou , Milinovich Gabriel , Xu Zhiwei , Bambrick Hilary , Mengersen Kerrie , Tong Shilu , Hu Wenbiao 
Published: August 2017

AbstractThis study aims to assess the utility of internet search query analysis in pertussis surveillance. This study uses an empirical time series model based on internet search metrics to detect the pertussis incidence in Australia. Our research demonstrates a clear seasonal pattern of both pertussis infections and Google Trends (GT) with specific search terms in time series seasonal decomposition analysis. The cross-correlation function showed significant correlations between GT and pertussis incidences in Australia and each state at the lag of 0 and 1 months, with the variation of correlations between 0.17 and 0.76 (p < 0.05). A multivariate seasonal autoregressive integrated moving average (SARIMA) model was developed to track pertussis epidemics pattern using GT data. Reflected values for this model were generally consistent with the observed values. The inclusion of GT metrics improved detective performance of the model (β = 0.058, p < 0.001). The validation analysis indicated that the overall agreement was 81% (sensitivity: 77% and specificity: 83%). This study demonstrates the feasibility of using internet search metrics for the detection of pertussis epidemics in real-time, which can be considered as a pre-requisite for constructing early warning systems for pertussis surveillance using internet search metrics.
Structural determinants of 5′,6′-epoxyeicosatrienoic acid binding to and activation of TRPV4 channel
Authors: Berna-Erro Alejandro , Izquierdo-Serra Mercè , Sepúlveda Romina V. , Rubio-Moscardo Fanny , Doñate-Macián Pau , Serra Selma A. , Carrillo-Garcia Julia , Perálvarez-Marín Alex , González-Nilo Fernando , Fernández-Fernández José M. , Valverde Miguel A. 
Published: August 2017

AbstractTRPV4 cation channel activation by cytochrome P450-mediated derivatives of arachidonic acid (AA), epoxyeicosatrienoic acids (EETs), constitute a major mechanisms of endothelium-derived vasodilatation. Besides, TRPV4 mechano/osmosensitivity depends on phospholipase A2(PLA2) activation and subsequent production of AA and EETs. However, the lack of evidence for a direct interaction of EETs with TRPV4 together with claims of EET-independent mechanical activation of TRPV4 has cast doubts on the validity of this mechanism. We now report: 1) The identification of an EET-binding pocket that specifically mediates TRPV4 activation by 5′,6′-EET, AA and hypotonic cell swelling, thereby suggesting that all these stimuli shared a common structural target within the TRPV4 channel; and 2) A structural insight into the gating of TRPV4 by a natural agonist (5′,6′-EET) in which K535 plays a crucial role, as mutant TRPV4-K535A losses binding of and gating by EET, without affecting GSK1016790A, 4α-phorbol 12,13-didecanoate and heat mediated channel activation. Together, our data demonstrates that the mechano- and osmotransducing messenger EET gates TRPV4 by a direct action on a site formed by residues from the S2-S3 linker, S4 and S4-S5 linker.
Injectable and thermosensitive TGF-β1-loaded PCEC hydrogel system for in vivo cartilage repair
Authors: Zhou Tengfei , Li Xiaolong , Li Guo , Tian Taoran , Lin Shiyu , Shi Sirong , Liao Jinfeng , Cai Xiaoxiao , Lin Yunfeng 
Published: August 2017

AbstractChondral defects pose a great challenge for clinicians to manage owing to the limited capacity for self-healing. Various traditional approaches have been adopted for the repair of these defects with unsatisfactory results. Cartilage tissue engineering techniques have emerged as promising strategies to enhance regeneration and overcome these traditional shortcomings. The cell-homing based technique is considered the most promising owing to its unique advantages. Thermosensitive hydrogels have been applied as scaffolds for biomedical applications with smart sol–gel response for altering environmental temperature. Transforming growth factor (TGF)-β1 is considered to be capable of promoting chondrogenesis. In this study, a novel TGF-β1-loaded poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCEC) hydrogel was fabricated using simple procedures. Hydrogel characterization, rheological testing, component analysis, and assessment of sol–gel transition, in vitro degradation, and TGF-β1 release confirmed that this material possesses a porous microstructure with favorable injectability and sustained drug release. Full-thickness cartilage defects were induced on rat knees for in vivo cartilage repair for eight weeks. Micro-CT and histological evaluation provided further evidence of the optimal capacity of this novel hydrogel for cartilage regeneration with respect to that of other methods. Moreover, our results demonstrated that the cell-free hydrogel is thermosensitive, injectable, biodegradable, and capable of in vivo cartilage repair and possesses high potential and benefits for acellular cartilage tissue engineering and clinical application in the future.
Biocompatible, large-format, inkjet printed heterostructure MoS2-graphene photodetectors on conformable substrates
Authors: Hossain Ridwan F. , Deaguero Isaac G. , Boland Thomas , Kaul Anupama B. 
Published: August 2017

AbstractAn inkjet printed, biocompatible, heterostructure photodetector is described that was constructed using inks of photo-active molybdenum disulfide (MoS2) and electrically conducting graphene which facilitated charge collection of the photocarriers. The importance of such devices stems from their potential utility in age-related-macular degeneration, which is a condition where the photosensitive retinal tissue degrades with aging, eventually compromising vision. The absence of effective therapeutic remedies for patients with this disorder has motivated the development of such devices to restore some degree of visual function. Inkjet printed, flexible prosthetic devices offer design simplicity where additive manufacturing can enable large format, low-cost arrays. The biocompatible inkjet printed two-dimensional heterojunction devices were photoresponsive to broadband incoming radiation in the visible regime, and the photocurrent Iph scaled proportionally with the incident light intensity, exhibiting a photoresponsivity R ~ 0.30 A/W. This is 103 times higher compared to prior reports, and detectivity D was calculated to be ~3.6 × 1010 Jones. Strain-dependent measurements were also conducted with bending, indicating the feasibility of such devices printed on flexible substrates. Drop cast and printed CT-MoS2 inks were characterized using techniques, such as Raman spectroscopy, photoluminescence measurements and scanning electron microscopy. Both mouse embryonic fibroblast and human esophageal fibroblast were used for the biocompatibility analysis for inks drop cast on two types of flexible substrates, polyethylene terephthalate and polyimide. The biocompatibility of inks formed using two-dimensional graphene and MoS2 on polyimide substrates was extremely high, in excess of 98% for mouse embryonic fibroblast.
Distributed Training Enhances Implicit Sequence Acquisition in Children With Specific Language Impairment
Authors: Desmottes Lise , Meulemans Thierry , Patinec Marie-Aude , Maillart Christelle 
Published: August 2017

Purpose
This study explored the effects of 2 different training structures on the implicit acquisition of a sequence in a serial reaction time (SRT) task in children with and without specific language impairment (SLI).

Method
All of the children underwent 3 training sessions, followed by a retention session 2 weeks after the last session. In the massed-training condition, the 3 training sessions were in immediate succession on 1 day, whereas in the distributed-training condition, the 3 training sessions were spread over a 1-week period in an expanding schedule format.

Results
Statistical analyses showed that the children with normal language were unaffected by the training conditions, performing the SRT task similarly in both training conditions. The children with SLI, however, were affected by the training structure, performing the SRT task better when the training sessions were spaced over time rather than clustered on 1 day.

Conclusion
This study demonstrated that although intensive training does not increase learning in children with SLI, distributing training sessions over time does increase learning. The implications of these results on the learning abilities of children with SLI are discussed, as are the mechanisms involved in massed versus distributed learning.
Gas kinematics and star formation in the filamentary molecular cloud G47.06+0.26
Authors: Xu Jin-Long , Xu Ye , Zhang Chuan-Peng , Liu Xiao-Lan , Yu Naiping , Ning Chang-Chun , Ju Bing-Gang 
Published: August 2017

Aims. We performed a multi-wavelength study toward the filamentary cloud G47.06+0.26 to investigate the gas kinematics and star formation.
Methods. We present the 12CO (J = 1−0), 13CO (J = 1−0) and C18O (J = 1−0) observations of G47.06+0.26 obtained with the Purple Mountain Observation (PMO) 13.7 m radio telescope to investigate the detailed kinematics of the filament. Radio continuum and infrared archival data were obtained from the NRAO VLA Sky Survey (NVSS), the APEX Telescope Large Area Survey of the Galaxy (ATLASGAL), the Galactic Legacy Infrared Mid-Plane Survey Extraordinaire (GLIMPSE) survey, and the Multi-band Imaging Photometer Survey of the Galaxy (MIPSGAL). To trace massive clumps and extract young stellar objects in G47.06+0.26, we used the BGPS catalog v2.0 and the GLIMPSE I catalog, respectively.
Results. The 12CO (J = 1−0) and 13CO (J = 1−0) emission of G47.06+0.26 appear to show a filamentary structure. The filament extends about 45′ (58.1 pc) along the east-west direction. The mean width is about 6.8 pc, as traced by the 13CO (J = 1−0) emission. G47.06+0.26 has a linear mass density of ~361.5 M⊙pc-1. The external pressure (due to neighboring bubbles and H II regions) may help preventing the filament from dispersing under the effects of turbulence. From the velocity-field map, we discern a velocity gradient perpendicular to G47.06+0.26. From the Bolocam Galactic Plane Survey (BGPS) catalog, we found nine BGPS sources in G47.06+0.26, that appear to these sources have sufficient mass to form massive stars. We obtained that the clump formation efficiency (CFE) is ~18% in the filament. Four infrared bubbles were found to be located in, and adjacent to, G47.06+0.26. Particularly, infrared bubble N98 shows a cometary structure. CO molecular gas adjacent to N98 also shows a very intense emission. H II regions associated with infrared bubbles can inject the energy to surrounding gas. We calculated the kinetic energy, ionization energy, and thermal energy of two H II regions in G47.06+0.26. From the GLIMPSE I catalog, we selected some Class I sources with an age of ~105 yr, which are clustered along the filament. The feedback from the H II regions may cause the formation of a new generation of stars in filament G47.06+0.26.
Bewegungskontrolle LWS
Authors: Oswald Oliver 
Published: August 2017

ZusammenfassungAssessments sind objektive standardisierte Testverfahren, mit denen sich in der
Physiotherapie die Situation des Patienten umfassend beurteilen lässt. Sie
liefern unverzichtbare Hinweise für die Optimierung und weitere Planung der
Therapie. Die AG Akademisierung des VPT stellt im VPTMAGAZIN valide Tests
steckbriefartig vor.
Exploding microbubbles driving a simple electrochemical micropump
Authors: Uvarov Ilia V , Lemekhov Sergey S , Melenev Artem E , Svetovoy Vitaly B 
Published: August 2017

AbstractElectrochemical microactuators and micropumps are too slow for many applications. The use of the alternating polarity electrolysis can strongly reduce the response time of such devices. We investigate a powerful pumping regime of a simple valveless micropump made from polydimethylsiloxane on a glass substrate. Microsecond dynamics of the gas bubbles in the chamber is monitored with fast cameras. After an incubation period of 10–100 ms a microbubble filling the entire chamber pops up in less than100 μs and disappears in 10 ms. This bubble pushes liquid out and drives the pump. The phenomenon is interpreted as an explosion of the microbubble containing a mixture of H2and O2gases. For higher amplitude of the driving pulses the incubation time can be as short as 1–2 ms but many uncorrelated microbubbles are formed in the chamber, and disappear in 1 ms. As the result a less powerful but faster pumping is possible. A few principles allowing further improve the micropump characteristics are formulated.
Decoding natural reach-and-grasp actions from human EEG
Authors: Schwarz Andreas , Ofner Patrick , Pereira Joana , Sburlea Andreea Ioana , Müller-Putz Gernot R 
Published: August 2017

Abstract

Objective. Despite the high number of degrees of freedom of the human hand, most actions of daily life can be executed incorporating only palmar, pincer and lateral grasp. In this study we attempt to discriminate these three different executed reach-and-grasp actions utilizing their EEG neural correlates. Approach. In a cue-guided experiment, 15 healthy individuals were asked to perform these actions using daily life objects. We recorded 72 trials for each reach-and-grasp condition and from a no-movement condition. Main results. Using low-frequency time domain features from 0.3 to 3 Hz, we achieved binary classification accuracies of 72.4%, STD  ±  5.8% between grasp types, for grasps versus no-movement condition peak performances of 93.5%, STD  ±  4.6% could be reached. In an offline multiclass classification scenario which incorporated not only all reach-and-grasp actions but also the no-movement condition, the highest performance could be reached using a window of 1000 ms for feature extraction. Classification performance peaked at 65.9%, STD  ±  8.1%. Underlying neural correlates of the reach-and-grasp actions, investigated over the primary motor cortex, showed significant differences starting from approximately 800 ms to 1200 ms after the movement onset which is also the same time frame where classification performance reached its maximum. Significance. We could show that it is possible to discriminate three executed reach-and-grasp actions prominent in people’s everyday use from non-invasive EEG. Underlying neural correlates showed significant differences between all tested conditions. These findings will eventually contribute to our attempt of controlling a neuroprosthesis in a natural and intuitive way, which could ultimately benefit motor impaired end users in their daily life actions.
Potentially 𝐺𝐿₂-type Galois representations associated to noncongruence modular forms
Authors: Li Wen-Ching , Liu Tong , Long Ling 
Published: August 2017

In this paper, we consider representations of the absolute Galois group

Gal
(

Q

¯

/

Q

)

\text {Gal}(\overline {\mathbb Q}/\mathbb Q)

attached to modular forms for noncongruence subgroups of

SL
2

(

Z

)

\text {SL}_2(\mathbb Z)

. When the underlying modular curves have a model over

Q

\mathbb {Q}

, these representations are constructed by Scholl in [Invent. Math. 99 (1985), pp. 49–77] and are referred to as Scholl representations, which form a large class of motivic Galois representations. In particular, by a result of Belyi, Scholl representations include the Galois actions on the Jacobian varieties of algebraic curves defined over

Q

\mathbb Q

. As Scholl representations are motivic, they are expected to correspond to automorphic representations according to the Langlands philosophy. Using recent developments on automorphy lifting theorem, we obtain various automorphy and potential automorphy results for potentially

G
L

2

\mathrm {GL}_2

-type Galois representations associated to noncongruence modular forms. Our results are applied to various kinds of examples. In particular, we obtain potential automorphy results for Galois representations attached to an infinite family of spaces of weight 3 noncongruence cusp forms of arbitrarily large dimensions.
CSAR: a contig scaffolding tool using algebraic rearrangements
Authors: Chen Kun-Tze , Liu Chia-Liang , Huang Shang-Hao , Shen Hsin-Ting , Shieh Yi-Kung , Chiu Hsien-Tai , Lu Chin Lung 
Published: August 2017

Abstract

Summary
Advances in next generation sequencing have generated massive amounts of short reads. However, assembling genome sequences from short reads still remains a challenging task. Due to errors in reads and large repeats in the genome, many of current assembly tools usually produce just collections of contigs whose relative positions and orientations along the genome being sequenced are still unknown. To address this issue, a scaffolding process to order and orient the contigs of a draft genome is needed for completing the genome sequence. In this work, we propose a new scaffolding tool called CSAR that can efficiently and more accurately order and orient the contigs of a given draft genome based on a reference genome of a related organism. In particular, the reference genome required by CSAR is not necessary to be complete in sequence. Our experimental results on real datasets have shown that CSAR outperforms other similar tools such as Projector2, OSLay and Mauve Aligner in terms of average sensitivity, precision, F-score, genome coverage, NGA50 and running time.

Availability and implementation
The program of CSAR can be downloaded from https://github.com/ablab-nthu/CSAR.

Supplementary information
Supplementary data are available at Bioinformatics online.
Pilot Study Examining the Safety and Efficacy of Calcium Hydroxylapatite Filler With Integral Lidocaine Over a 12-Month Period to Correct Temporal Fossa Volume Loss
Authors: Juhász Margit L.W. , Levin Melissa K. , Marmur Ellen S. 
Published: August 2017

BACKGROUND
Age-related volume loss in the temporal fossae is due to thinning of the epidermis, loss of subcutaneous structural volume, and change in the bony architecture. Temporal concavities are important areas of 3-dimensional volume restoration. The temporal fossae is becoming an increasingly popular area for patients seeking soft tissue augmentation with injectable fillers such as calcium hydroxylapatite with integral lidocaine [CaHA (+)].

OBJECTIVE
This pilot study aims to define the safety, efficacy, technique, and patient-reported outcomes for injectable CaHA (+) to correct volume loss in the temporal fossae over a 12-month period.

MATERIALS AND METHODS
This was a single-investigator, nonblinded study involving 20 participants. Participants received filler injection into their temporal fossae, with follow-up evaluations at Day 14, 6 weeks, and 3, 6, 9, and 12 months.

RESULTS
CaHA (+) results in statistically significant improvement in temporal fossae appearance lasting up to 12 months. Subjects reported “moderate” global aesthetic improvement over the 12-month period.

CONCLUSION
As the cosmetic field continues to advance, it is important for practitioners to have access to research regarding the efficacy and safety of injectables. These results show that CaHA (+) is an effective and safe option to correct temporal fossae volume loss associated with high patient satisfaction.
Male Body Contouring
Authors: Coleman Kyle M. , Lawrence Naomi 
Published: August 2017

BACKGROUND
Men are increasingly seeking out cosmetic procedures, especially minimally and noninvasive body-contouring procedures.

OBJECTIVE
With the relative lack of scientific evidence related specifically to the use of body-contouring procedures in men, there is a need for more education and scientific discussion in this growing group.

MATERIALS AND METHODS
Understanding the male anatomy and aesthetics, and how body-contouring techniques and new modalities can be used in men, can lead to better outcomes.

CONCLUSIONS
This review of body contouring in men emphasizes currently available literature and author experiences.
BLEB-RELATED ENDOPHTHALMITIS AFTER USE OF THE INVITRIA INJECTION GUIDE
Authors: Baneke Alex J. , Vakros Georgios , Sharma Vik , Wong Sui Chien 
Published: August 2017

Purpose:
To present the case of a patient who developed bleb-related endophthalmitis after an intravitreal injection using the InVitria injection guide, alongside a review of the literature on bleb-related endophthalmitis.

Methods:
Our case is presented in context of the current literature on bleb-related endophthalmitis.

Results:
Bleb-related endophthalmitis occurs in up to 2% of eyes within 5 years of filtering glaucoma surgery. Risk factors include bleb leakage, use of anti-fibrinolytic agents, blepharitis, hypotony, and nasolacrimal duct obstruction. Our patient presented with a bleb leak and bleb-related endophthalmitis 2 weeks after an intravitreal injection using the InVitria injection guide.

Conclusion:
Injection guides can cause bleb trauma and resulting bleb leakage. They should be avoided in patients with filtering glaucoma surgery.