bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kif2 microtubule depolymerase is required for unequal cell division and localizes to a subdomain of cortical endoplasmic reticulum
Authors: Costache Vlad , Hebras Celine , Pruliere Gerard , Besnardeau Lydia , Failla Margaux , Copley Richard R. , Burgess David , Chenevert Janet , McDougall Alex 
Published: August 2017

AbstractUnequal cell division (UCD) is a fundamental process responsible for creating sibling cell size asymmetry; however, how microtubules are specifically depolymerized on one aster of the mitotic spindle creating a smaller sibling cell during UCD has remained elusive. Using invertebrate chordate embryos (ascidian) that possess a large cortical structure (CAB) that causes UCD, we identified a microtubule depolymerase (Kif2) involved in creating cell size asymmetry. Kif2 localizes to the cortical subdomain of endoplasmic reticulum in the CAB. During three successive UCDs, Kif2 protein accumulates at the CAB during interphase and is delocalized from the CAB in mid mitosis. Rapid imaging of microtubule dynamics at the cortex revealed that microtubules do not penetrate the CAB during interphase. Inhibition of Kif2 function prevents the development of mitotic aster asymmetry and centrosome movement towards the CAB thereby blocking UCD, whereas locally increasing microtubule depolymerization causes exaggerated asymmetric spindle positioning. This study provides insights into the fundamental process of UCD and for the first time shows that a microtubule depolymerase is localized to a cortical site controlling UCD.
Intronic CNVs cause gene expression variation in human populations
Authors: Rigau Maria , Juan David , Valencia Alfonso , Rico Daniel 
Published: August 2017

AbstractIntrons can be extraordinarily large and they account for the majority of the DNA sequence in human genes. However, little is known about their population patterns of structural variation and their functional implication. By combining the most extensive maps of CNVs in human populations, we have found that intronic losses are the most frequent copy number variants (CNVs) in protein-coding genes in human, with more than 12,986 intronic deletions, affecting 4,147 genes (including 1,154 essential genes and 1,638 disease-related genes). These intronic length variation results in dozens of genes showing extreme population variability in size, with 40 genes with 10 or more different sizes and up to 150 allelic sizes. Intronic losses are frequent in evolutionarily ancient genes that are highly conserved at the protein sequence level. This result contrasts with losses overlapping exons, which are observed less often than expected by chance and almost exclusively affect primate-specific genes. An integrated analysis of CNVs and RNA-seq data showed that intronic loss can be associated with significant differences in gene expression levels in the population (CNV-eQTLs). These intronic CNV-eQTLs regions are enriched for intronic enhancers and can be associated with expression differences of other genes showing long distance intron-promoter 3D interactions. Our data suggests that intronic structural variation of protein-coding genes can exert an important role in maintaining the variability of gene expression and splicing in human populations.Author SummaryMost human genes have introns that have to be removed after a gene is transcribed from DNA to RNA because they not encode information to translate RNA into proteins. As mutations in introns do not affect protein sequences, they are usually ignored when looking for normal or pathogenic genomic variation. However, introns comprise about half of the human non-coding genome and they can have important regulatory roles. We show that deletions of intronic regions appear more frequent than previously expected in the healthy population, with a significant proportion of genes with evolutionary ancient and essential functions carrying them. This finding was very surprising, as ancient genes tend to have high conservation of their coding sequence. However, we show that deletions of their non-coding intronic sequence can produce considerable changes in their locus length. We found that a significant number of these intronic deletions are associated with under- or over-expression of the affected genes or distant genes interacting in 3D. Our data suggests that the frequent gene length variation in protein-coding genes resulting from intronic CNVs might influence their regulation in different individuals.
QuimP – Analyzing transmembrane signalling in highly deformable cells
Authors: Baniukiewicz Piotr , Collier Sharon , Bretschneider Till 
Published: August 2017

AbstractSummaryTransmembrane signalling plays important physiological roles, with G protein–coupled cell surface receptors being particularly important therapeutic targets. Fluorescent proteins are widely used to study signalling, but the analysis of image time series can be challenging, in particular when changes in cell shape are involved. To this end we have developed QuimP software. QuimP semi-automatically tracks cell outlines, quantifies spatio-temporal patterns of fluorescence at the cell membrane, and tracks local shape deformations. QuimP is particularly useful for studying cell motility, for example in immune or cancer cells.Availability and ImplementationQuimP (http://warwick.ac.uk/quimp) consists of a set of Java plugins for Fiji/ImageJ (http://fiji.sc/) and can be easily installed through the Fiji Updater (http://warwick.ac.uk/quimp/wiki-pages/installation). It is compatible with Mac, Windows and Unix-based operating systems, requiring version >1.45 of Fiji/ImageJ and Java 8. QuimP is released as open source (https://github.com/CellDynamics/QuimP/) under an academic licence.ContactT.Bretschneider@warwick.ac.ukSupplementary InformationSupplementary materials (SI-A to SI-D) are available at Bioinformatics online. Test data is available fromhttp://warwick.ac.uk/quimp/test_data.
Extracranial estimation of neural mass model parameters using the Unscented Kalman Filter
Authors: Escuain-Poole Lara , Garcia-Ojalvo Jordi , Pons Antonio J. 
Published: August 2017

AbstractData assimilation, defined as the fusion of data with preexisting knowledge, is particularly suited to elucidating underlying phenomena from noisy/insufficient observations. Although this approach has been widely used in diverse fields, only recently have efforts been directed to problems in neuroscience, using mainly intracranial data and thus limiting its applicability to invasive measurements involving electrode implants. Here we intend to apply data assimilation to non-invasive electroencephalography (EEG) measurements to infer brain states and their characteristics. For this purpose, we use Kalman filtering to combine synthetic EEG data with a coupled neural-mass model together with Ary’s model of the head, which projects intracranial signals onto the scalp. Our results show that using several extracranial electrodes allows to successfully estimate the state and parameters of the neural masses and their interactions, whereas one single electrode provides only a very partial and insufficient view of the system. The superiority of using multiple extracranial electrodes over using only one, be it intra- or extracranial, is shown over a wide variety of dynamical behaviours. Our results show potential towards future clinical applications of the method.Author SummaryTo completely understand brain function, we will need to integrate experimental information into a consistent theoretical framework. Invasive techniques as EcoG recordings, together with models that describe the brain at the mesoscale, provide valuable information about the brain state and its dynamical evolution when combined with techniques coming from control theory, such as the Kalman filter. This method, which is specifically designed to deal with systems with noisy or imperfect data, combines experimental data with theoretical models assuming Bayesian inference. So far, implementations of the Kalman filter have not been suited for non-invasive measures like EEG. Here we attempt to overcome this situation by introducing a model of the head that allows to transfer the intracranial signals produced by a mesoscopic model to the scalp in the form of EEG recordings. Our results show the advantages of using multichannel EEG recordings, which are extended in space and allow to discriminate signals produced by the interaction of coupled columns. The extension of the Kalman method presented here can be expected to expand the applicability of the technique to all situations where EEG recordings are used, including the routine monitoring of illnesses or rehabilitation tasks, brain-computer interface protocols, and transcranial stimulation.
Sugar intake elicits a small-scale search behavior in flies and honey bees that involves capabilities found in large-scale navigation
Authors: Brockmann Axel , Murata Satoshi , Murashima Naomi , Boyapati Ravi Kumar , Shakeel Manal , Prabhu Nikhil G. , Herman Jacob J. , Basu Pallab , Tanimura Teiichi 
Published: August 2017

AbstractSocial insects, particularly bees and ants, show exceptional large-scale navigational skills to find and carry back food to their nests. Honey bees further evolved a symbolic communication to direct nest mates to attractive food sources. Till now it is unclear how these capabilities evolved. Sixty years ago, Vincent Dethier demonstrated that a small-scale sugar-elicited search behavior identified in flies shows remarkable similarities with honey bee dance behavior. Those findings suggested that both behaviors are based on common mechanisms and are likely evolutionary related. We now present for the first time a detailed comparison of the sugar-elicited search behavior inDrosophila melanogasterandApis mellifera. In both species, intake of sugar elicits a complex of searching responses. The most obvious response was an increase in turning frequency, but more importantly we found that flies and honey bees returned to the location of the sugar drop. They even returned to the food location when we prevented them from using visual and chemosensory cues indicating that this small scale local search involves path integration mechanisms. Finally, we show that visual landmarks presented in the vicinity of the sugar drop affected the search trajectory and in honey bees the sugar intake induced learning of landmarks. Together, our experiments indicate that the sugar-elicited local search exhibits two major behavioral capabilities of large-scale navigation, path integration and landmark orientation.Significance StatementTo search for food social insects evolved sophisticated strategies of spatial orientation and large-scale navigation. We now show that even a small-scale local search behavior in solitary flies and social honey bees involves path integration and landmark learning two major mechanisms of large-scale navigation. We propose that in the future sugar-elicited local search can be used to identify neural circuits involved in navigation, path integration, and landmark learning.
TRuML: A Translator for Rule-Based Modeling Languages
Authors: Suderman Ryan , Hlavacek William S. 
Published: August 2017

ABSTRACTRule-based modeling languages, such as the Kappa and BioNetGen languages (BNGL), are powerful frameworks for modeling the dynamics of complex biochemical reaction networks. Each language is distributed with a distinct software suite and modelers may wish to take advantage of both toolsets. This paper introduces a practical application called TRuML that translates models written in either Kappa or BNGL into the other language. While similar in many respects, key differences between the two languages makes translation sufficiently complex that automation becomes a useful tool. TRuML accommodates the languages’ complexities and produces a semantically equivalent model in the alternate language of the input model when possible and an approximate model in certain other cases. Here, we discuss a number of these complexities and provide examples of equivalent models in both Kappa and BNGL.CCS CONCEPTS•Applied computing→Systems biology; •Computing methodologies→Simulation languages;
OYO Rooms: providing affordable hotel stays
Authors: Sharma Tripti Ghosh , Jain Rohit , Kapoor Sahil , Gaur Vijeyta , Roy Abhishek 
Published: August 2017

Subject area
Strategic Marketing, Marketing Management, Services Marketing.

Study level/applicability
MBA and Executive MBA.

Case overview
The case talks about the inception and growth of OYO Rooms, a company that originally started as ORAVEL Stays Ltd. in 2012, as a platform for booking budget and premium accommodations, but graduated to become OYO Rooms, an online aggregator of hotels, with a unique business model of “managing the partial inventory of rooms” in hotels and offering a proposition of affordable, consistent, quality experience to business, leisure and pilgrim travellers. The company received rounds of funding from Greenoaks Capital, Lightspeed Ventures, Sequoia Capital and DSG Consumer Partners. Moreover, unlike its competitors, OYO adapted itself to the fast-changing consumer preference and grew at an enviable pace and by 2016, was present across 190 cities through a network of 6,500 hotels. However, OYO Rooms had to face a multitude of challenges both from the consumer and hotel owners’ ends, primarily service quality concerns from the customers and majorly concerns out of payment irregularities or non-abidance to written contracts from the hoteliers’ end. The dissatisfaction levels increased to an extent that experts started raising questions on the viability of the business. OYO was growing at an aggressive rate but breakeven point was yet to be achieved. Moreover, growing dissatisfaction and switching amongst its customers as well as hoteliers threatened the very existence of the model. The case allows the students to critically analyse the strategies of OYO for deliberation on whether the business model was sustainable in the long run. It also encourages the students to deliberate on the possible growth strategies for OYO as also on the service recovery strategies for OYO.

Expected learning outcomes
The case has been positioned around the following modules: industry analysis; value of a two-sided business model to both parties; sustainability of a unique business model, against the challenges that it faces; applying the VRIO framework (resource-based view); complaint handling and service recovery strategies; applying the Ansoff’s grid for possible growth options.

Supplementary materials
Teaching notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 11: Strategy.
Double edge experiences of expatriate acculturation
Authors: Pekerti Andre Anugerah , Vuong Quan Hoang , Napier Nancy K. 
Published: August 2017

PurposeThe purpose of this paper is to bring to light the double edges faced by individuals who have international and multicultural experiences. The implication is that these individuals encounter acculturation challenges, and also gain from their multiculturality. The authors adopt Berry’s (2011) integration and multiculturalism framework to analyze the experiences and challenges that multi-culturals face. This paper suggests ways to glean the silver lining within organizations to help manage and master multicultural experiences in the workplace to benefit both individuals and organizations.Design/methodology/approachThe authors used empirical materials from expatriates who have worked across multiple cultural contexts. Based on these the authors present three examples to illustrate how expatriates and multicultural individuals place themselves in situations where they experience contact and challenges associated with adopting multiple cultures. The authors then analyze these examples to show how the experiences involve psychological-level integration challenges for Multi- andn-culturals.FindingsThe three multicultural expatriate examples suggest that individuals with international and multicultural experiences who are successful at managing their experiences develop cognitive and behavioral complexity. However, these individuals also face continuous acculturation including cognitive and ethno-cultural identity conflicts such as, rejection from multiple cultural perspectives because they continually cross-multiple cultural microcosms. Suggestions are presented to help maintain one’s sense of self-worth and minimizing ethno-cultural conflicts.Research limitations/implicationsNotwithstanding the value of analyzing the examples of expatriate acculturation experiences, the limitation to the examples is that it is limited to the experience of three individuals. However, the examples were effective in raising points to discuss relevant challenges and/or the double-edged reality faced by boundary spanners, multi-, andn-culturals.Practical implicationsThe paper presents possible ways multi- andn-culturals navigate through their multiculturalism, including suggestions to help individuals who struggle with their multiculturalism through mentoring.Social implicationsThe paper highlights the challenges of acculturation and suggests ways that individuals can overcome these challenges. It further suggests how organizations can take advantage of such individuals by utilizing existing personnel within the organization.Originality/valueThe paper is one of the few that acknowledge multiculturalism is highly challenging even for successful multi-culturals andn-culturals. Currently the literature is scant concerning how individuals can manage and master multicultural experiences in the workplace. The paper suggests a number of useful strategies for individuals and organizations to manage the challenges.
Corporate social responsibility and payout decisions
Authors: Samet Marwa , Jarboui Anis 
Published: August 2017

PurposeThe purpose of this paper is to investigate whether and how corporate social responsibility (CSR) performance contributes to shape firms’ payout policy. In particular, it examines the influence of CSR performance on payout level and payout channel choice (dividend payment or share repurchases). Additionally, it examines the moderating role of CSR performance in the relationship between dividends and share repurchases.Design/methodology/approachUsing 397 European companies listed in the STOXX Europe 600 over the period from 2009 to 2014, the authors employ regression analysis to explore the link between CSR performance and payout policy.FindingsThe first result shows that firms with high CSR performance engage more in payout policy. Second, when choosing between paying dividends and repurchasing stocks, firms with high CSR performance tend to prefer share repurchases. Finally, CSR performance plays an important role in determining the relationship between dividends and repurchases. Specifically, dividends and share repurchases seem to be more substitutable among socially responsible firms.Practical implicationsFirms that are able to develop successful CSR strategies can generate tangible benefits for their shareholders in the form of high payout levels. An increase in CSR expenditure does not lead to cut or minimize the cash flow paid out to shareholders. In addition, government and regulators have to oblige or at least encourage socially responsible firms to use executive stock option that are dividend protected, in order to reduce distortions in dividend policy.Originality/valueThis is the first attempt to investigate the association between CSR performance and share repurchase activities.
Board structure and institutional ownership at the time of IPO
Authors: Neupane Suman , Neupane Biwesh 
Published: August 2017

Purpose
The purpose of this paper is to examine the impact of mandatory regulatory provisions on board structure and the influence of such board structure on institutional holdings.

Design/methodology/approach
The study uses unique hand-collected data set of Indian IPOs during the 2004-2012 period after the corporate governance reforms with the introduction of clause 49 in the listing agreements in 2001. Using OLS regression, the paper empirically analyses the determinants of board size and board independence at the time of the IPOs and the influence of such a board structure on shareholdings by domestic and foreign institutional investors.

Findings
The authors find that complying with mandatory regulatory provisions does not impede firms from structuring their boards to reflect the firms’ advising and monitoring needs. The authors also find that complying with provisions have positive implication for the firm, as firms with greater board independence appear to attract more foreign institutional investors.

Originality/value
To the authors’ best knowledge, this is the first study to examine the issue in a regime where regulation mandates the composition of the board of directors. The paper also extends the literature on institutional holdings by providing evidence on the impact of board structure on institutional ownership at a critical time in a firm’s life cycle when concerns for endogeneity for empirical investigations are weaker.
Psychophysiological Responses of People with Psychopathic Tendencies to the Concealed Information Test
Authors: Hong Hyeon‐Gi , Kim Hee‐Song , Ji Hyung‐Ki , Kim Ki‐Pyoung , Lee Jungeun , Jung Sue Hyun , Hyun Myoung‐Ho 
Published: August 2017

AbstractThis study was designed to investigate differences in psychophysiological responses among individuals with psychopathic tendencies. A total 40 students were participated and were divided into two groups based on Levenson Self‐Report Psychopathy Scale (LSRP) scores: a high psychopathic tendency group selected from the top 5% of LSRP scores, and a low psychopathic tendency group selected from the bottom 5% of LSRP scores. All participants were instructed to perform a mock crime and complete the concealed information test (CIT). Results indicate that significant differences between crime‐relevant questions and irrelevant questions were observed for respiration line length and skin conductance response in both groups, but heart rate did not significantly differ between groups. There were no differences in psychophysiological responses between the groups. In conclusion, CIT was found to be an effective method for detecting crime‐relevant information in individuals with psychopathic tendencies.
Fatal Diabetic Ketoacidosis—A Potential Complication of MDMA (Ecstasy) Use
Authors: Gilbert John D. , Byard Roger W. 
Published: August 2017

AbstractA 19‐year‐old woman with insulin‐dependent diabetes mellitus was found dead in bed having allegedly recently taken ecstasy and consumed alcohol. At autopsy, there were microhemorrhages in the brain with subnuclear vacuolization and Armanni–Ebstein changes in renal tubules. Biochemical analyses confirmed diabetic ketoacidosis (vitreous glucose—46.5 mmol/L; β‐OH butyrate—13.86 mmol/L.). Toxicological analyses of blood showed a low level of 3,4‐methylenedioxy‐methamphetamine (MDMA) (0.01 mg/L), with acetone but no alcohol or other common drugs. Death was attributed to diabetic ketoacidosis most likely provoked by mixed MDMA/alcohol ingestion. Although the use of illicit drugs by young individuals with diabetes mellitus is being increasingly recognized, it has been noted that there is minimal information about the relationship between drug use and acute diabetic complications. Toxicological screening of cases of lethal diabetic ketoacidosis in the young may clarify lethal mechanisms in individual cases and also help to determine the extent of this problem.