bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a multiplex probe combination-based one-step real-time reverse transcription-PCR for NA subtype typing of avian influenza virus
Authors: Sun Zhihao , Qin Tao , Meng Feifei , Chen Sujuan , Peng Daxin , Liu Xiufan 
Published: October 2017

AbstractNine influenza virus neuraminidase (NA) subtypes have been identified in poultry and wild birds. Few methods are available for rapid and simple NA subtyping. Here we developed a multiplex probe combination-based one-step real-time reverse transcriptase PCR (rRT-PCR) to detect nine avian influenza virus NA subtypes. Nine primer-probe pairs were assigned to three groups based on the different fluorescent dyes of the probes (FAM, HEX, or Texas Red). Each probe detected only one NA subtype, without cross reactivity. The detection limit was less than 100 EID50 or 100 copies of cDNA per reaction. Data obtained using this method with allantoic fluid samples isolated from live bird markets and H9N2-infected chickens correlated well with data obtained using virus isolation and sequencing, but was more sensitive. This new method provides a specific and sensitive alternative to conventional NA-subtyping methods.
Up-regulation of casein kinase 1ε is involved in tau pathogenesis in Alzheimer’s disease
Authors: Chen Caoyi , Gu Jianlan , Basurto-Islas Gustavo , Jin Nana , Wu Feng , Gong Cheng-Xin , Iqbal Khalid , Liu Fei 
Published: October 2017

AbstractHyperphosphorylation of tau and imbalanced expression of 3R-tau and 4R-tau as a result of dysregulation of tau exon 10 splicing are believed to be pivotal to the pathogenesis of tau pathology, but the molecular mechanism leading to the pathologic tau formation in Alzheimer’s disease (AD) brain is not fully understood. In the present study, we found that casein kinase 1ε (CK1ε) was increased significantly in AD brains. Overexpression of CK1ε in cultured cells led to increased tau phosphorylation at many sites. Moreover, we found that CK1ε suppressed tau exon 10 inclusion. Levels of CK1ε were positively correlated to tau phosphorylation, 3R-tau expression and tau pathology, and negatively correlated to 4R-tau in AD brains. Overexpression of CK1ε in the mouse hippocampus increased tau phosphorylation and impaired spontaneous alternation behavior. These data suggest that CK1ε is involved in the regulation of tau phosphorylation, the alternative splicing of tau exon 10, and cognitive performance. Up-regulation of CK1ε might contribute to tau pathology by hyperphosphorylating tau and by dysregulating the alternative splicing of tau exon 10 in AD.
Emergence of the Stoner-Wohlfarth astroid in thin films at dynamic regime
Authors: Cuñado José Luis F. , Bollero Alberto , Pérez-Castañeda Tomás , Perna Paolo , Ajejas Fernando , Pedrosa Javier , Gudín Adrian , Maldonado Ana , Niño Miguel Angel , Guerrero Rubén , Cabrera David , Terán Francisco J. , Miranda Rodolfo , Camarero Julio 
Published: October 2017

AbstractThe Stoner-Wohlfarth (SW) model is the simplest model that describes adequately the magnetization reversal of nanoscale systems that are small enough to contain single magnetic domains. However for larger sizes where multi-domain effects are present, e.g., in thin films, this simple macrospin approximation fails and the experimental critical curve, referred as SW astroid, is far from its predictions. Here we show that this discrepancy could vanish also in extended system. We present a detailed angular-dependent study of magnetization reversal dynamics of a thin film with well-defined uniaxial magnetic anisotropy, performed over 9 decades of applied field sweep rate (dH/dt). The angular-dependent properties display a gradual transition from domain wall pinning and motion-like behaviour to a nucleative single-particle one, as dH/dtincreases. Remarkably, in the high dynamic regime, where nucleation of reversed domains is the dominant mechanism of the magnetization reversal (nucleative regime), the magnetic properties including the astroid become closer to the ones predicted by SW model. The results also show why the SW model can successfully describe other extended systems that present nucleative regime, even in quasi-static conditions.
Impact of heavy metal lead stress on polyamine levels in Halomonas BVR 1 isolated from an industry effluent
Authors: Manasi  , Mohapatra Sridev , Rajesh N. , Rajesh Vidya 
Published: October 2017

AbstractIn living systems, environmental stress due to biotic and abiotic factors triggers the production of myriad metabolites as a potential mechanism for combating stress. Among these metabolites are the small polycationic aliphatic amine molecules - polyamines, which are ubiquitous in all living organisms. In this work, we demonstrate a correlation between cellular concentration of three major polyamines (putrescine, spermidine and spermine) with lead exposure on bacteria for a period of 6–24 h. We report that indigenously isolated Halomonas sp. strain BVR 1 exhibits lead induced fluctuations in their cellular polyamine concentration. This response to lead occurs within 6 h post metal treatment. During the same time interval there was a surge in the growth of bacteria along with an enhancement in the putrescine levels. We conclude that in Halomonas sp. strain BVR 1, an early response is seen with respect to modulation of polyamines as a result of lead treatment and hypothesize that endogenous polyamines contribute towards scavenging lead in these bacteria.
Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction
Authors: Cheon So Yeong , Kim Jeong Min , Kam Eun Hee , Ho Chun-Chang , Kim Eun Jung , Chung Seungsoo , Jeong Ji-Hyun , Lee Diane Da-Hyun , Lee Sang-Won , Koo Bon-Nyeo 
Published: October 2017

AbstractSome patients experience impaired cognitive functioning after surgery, a phenomenon referred to as postoperative cognitive dysfunction (POCD). Signs of POCD are closely associated with the development of systemic or hippocampal inflammation. However, the precise pathophysiological mechanisms of prevention/treatment options for POCD still remain unclear. After injury, the transcriptional factor nuclear factor-kappa B (NF-κB) is thought to regulate or stimulate inflammation amplification. Therefore, we designed a cell-penetrating fusion protein called nt-p65-TMD, which inhibits NF-κB p65 activation by translocating into the nucleus. In the present study, we discovered that nt-p65-TMD exerted effects on surgery-induced cognitive impairment in mice. Specifically, nt-p65-TMD exhibited strong immunoregulatory properties that were able to reduce surgery-induced elevations in cerebrovascular integrity impairment, subsequent peripheral immune-cell recruitment, and inflammation amplification, which ultimately lead to cognitive decline. The nt-p65-TMD has the unique ability to regulate and reduce systemic inflammation and inflammation amplification, suggesting a new strategy for preventing development of cognitive decline that occurs in POCD.
Downregulation of miR-16 via URGCP pathway contributes to glioma growth
Authors: Hong Liang , Qing Ouyang , Ji Zhou , Chengqu Zhang , Ying Chen , Hao Cui , Minhui Xu , Lunshan Xu 
Published: October 2017

AbstractExperimental and clinical evidence points to a critical role of Upregulator of cell proliferation (URGCP/URG4) in controlling the progression of multiple tumors. However, the oncogenic role of URGCP in glioma still remains elusive. In this study we tried to investigate the oncogenic roles and molecular mechanisms of URGCP in glioma. We found that the levels of URGCP were upregulated in glioma, and that the high-levels of URGCP indicated a worse prognosis in glioma patients. URGCP and miR-16 are critical for glioma growth: silencing URGCP (shURGCP) inhibited glioma growth, while, the shURGCP-mediated proliferative inhibition could be recovered by antagonizing miR-16 (anta-miR-16) in vivo and in vitro. Mechanically, URGCP repressed miR-16 expression via activating NF-κB/c-myc pathway in glioma; Cyclins D1 and Cyclin E1 were identified as the direct targets of miR-16, thus, URGCP-mediated miR-16 downregulation accelerated cell proliferation by upregulating Cyclin D1 and Cyclin E1 expression. All these results suggested that URGCP accelerates glioma growth through the NF-κB/c-myc/miR-16/Cyclin D1/E1 pathway, and both URGCP and miR-16 function as a novel cell cycle regulators in glioma and could be considered as potential targets for glioma therapy.
Enhancing Ear and Hearing Health Access for Children With Technology and Connectivity
Authors: Swanepoel De Wet 
Published: October 2017

Purpose
Technology and connectivity advances are demonstrating increasing potential to improve access of service delivery to persons with hearing loss. This article demonstrates use cases from community-based hearing screening and automated diagnosis of ear disease.

Method
This brief report reviews recent evidence for school- and home-based hearing testing in underserved communities using smartphone technologies paired with calibrated headphones. Another area of potential impact facilitated by technology and connectivity is the use of feature extraction algorithms to facilitate automated diagnosis of most common ear conditions from video-otoscopic images.

Results
Smartphone hearing screening using calibrated headphones demonstrated equivalent sensitivity and specificity for school-based hearing screening. Automating test sequences with a forced-choice response paradigm allowed persons with minimal training to offer screening in underserved communities. The automated image analysis and diagnosis system for ear disease demonstrated an overall accuracy of 80.6%, which is up to par and exceeds accuracy rates previously reported for general practitioners and pediatricians.

Conclusion
The emergence of these tools that capitalize on technology and connectivity advances enables affordable and accessible models of service delivery for community-based ear and hearing care.
Impact of the road network configuration on map‐matching algorithms for FCD in urban environments
Authors: Arregui Harbil , Loyo Estibaliz , Otaegui Oihana , Arbelaitz Olatz 
Published: October 2017

Novel ubiquitous traffic sensors such as floating car data (FCD) are getting extended due to the use of 24 h connected smartphones and global positioning systems. Road conditions such as travel speeds in each road link and mobility demand can be monitored by measurements coming from moving vehicles consisting of geolocation and speed information with timestamps. Map‐matching is the process needed to identify the corresponding road link on a digital map and define the position of the geolocated vehicle on this link, overcoming positioning errors. Matching processes in urban environments are more prone to error due to the topology and features of city road networks. In this study, the accuracy of the map‐matching is discussed depending on the road configuration for FCD in urban and interurban scenarios, under sampling frequencies ranging from 5 to 60 s. Concretely, in this analysis, three matching techniques have been evaluated against road density, nominative speed limit, edge length and edge count values in order to quantify the impact of these variables on the matching accuracy.
The XXL Survey
Authors: Guglielmo V. , Poggianti B. M. , Vulcani B. , Adami C. , Gastaldello F. , Ettori S. , Fotoupoulou S. , Koulouridis E. , Ramos Ceja M. E. , Giles P. , McGee S. , Altieri B. , Baldry I. , Birkinshaw M. , Bolzonella M. , Bongiorno A. , Brown M. , Chiappetti L. , Driver S. , Elyiv A. , Evrard A. , Garilli B. , Grootes M. , Guennou L. , Hopkins A. , Horellou C. , Iovino A. , Lidman C. , Liske J. , Maurogordato S. , Owers M. , Pacaud F. , Paltani S. , Pierre M. , Plionis M. , Ponman T. , Robotham A. , Sadibekova T. , Scodeggio M. , Sereno M. , Smolčić V. , Tuffs R. , Valtchanov I. , Vignali C. , Willis J. 
Published: October 2017

Context. The fraction of galaxies bound in groups in the nearby Universe is high (50% at z ~ 0). Systematic studies of galaxy properties in groups are important in order to improve our understanding of the evolution of galaxies and of the physical phenomena occurring within this environment.
Aims. We have built a complete spectrophotometric sample of galaxies within X-ray detected, optically spectroscopically confirmed groups and clusters (G&C), covering a wide range of halo masses at z ≤ 0.6.
Methods. In the context of the XXL survey, we analyse a sample of 164 G&C in the XXL-North region (XXL-N), at z ≤ 0.6, with a wide range of virial masses (1.24 × 1013 ≤ M500,scal(M⊙) ≤ 6.63 × 1014) and X-ray luminosities ((2.27 × 1041 ≤ L500,scalXXL(erg s−1) ≤ 2.15 × 1044)). The G&C are X-ray selected and spectroscopically confirmed. We describe the membership assignment and the spectroscopic completeness analysis, and compute stellar masses. As a first scientific exploitation of the sample, we study the dependence of the galaxy stellar mass function (GSMF) on global environment.
Results. We present a spectrophotometric characterisation of the G&C and their galaxies. The final sample contains 132 G&C, 22 111 field galaxies and 2225 G&C galaxies with r-band magnitude <20. Of the G&C, 95% have at least three spectroscopic members, and 70% at least ten. The shape of the GSMF seems not to depend on environment (field versus G&C) or X-ray luminosity (used as a proxy for the virial mass of the system). These results are confirmed by the study of the correlation between mean stellar mass of G&C members and L500,scalXXL. We release the spectrophotometric catalogue of galaxies with all the quantities computed in this work.
Conclusions. As a first homogeneous census of galaxies within X-ray spectroscopically confirmed G&C at these redshifts, this sample will allow environmental studies of the evolution of galaxy properties.
“Drowning Brain in a Pool of CSF” — A Rare Complication of Periencephalic Subdural Panhygroma following Removal of a Posterior Fossa Tumor
Authors: Behera Bikash , Deo Ram , Mishra Sanjib , Biswal Jyotirmayee , Das Deepak 
Published: October 2017

AbstractPosterior fossa tumors are commonly encountered in pediatric age group patients. Most of these tumors present with features of hydrocephalus in the child. Conventionally, these cases are managed by suboccipital craniotomy with decompression of the tumor mass to establish the free flow of cerebrospinal fluid (CSF) across fourth ventricle and aqueduct of Sylvius. Following resection of posterior fossa tumor, appearance of subdural hygroma is a rare phenomenon.Though few cases of subdural hygroma are reported in literature following foramen magnum decompression in Chiari's malformation, their appearance following posterior fossa tumor resection is alien to medical literature and limited to only two case reports. Here the authors present a patient with periencephalic subdural panhygroma (PSP) following posterior fossa tumor resection who was successfully treated with a ventriculoperitoneal shunt (VPS) to accomplish a symptomatic and radiologic remission.
Leaps in Technology: Advanced MR Imaging after Total Hip Arthroplasty
Authors: Khodarahmi Iman , Nittka Mathias , Fritz Jan 
Published: October 2017

AbstractThe vast majority of the metal-related artifacts in magnetic resonance imaging (MRI) arise from B0 inhomogeneity. These artifacts include failed fat suppression, signal loss, signal pileup, and image distortions. Metal artifact reduction sequence MRI has been used to mitigate these artifacts via optimization of the scan parameters and exploiting new techniques such as fully phase-encoded imaging and multispectral imaging including multi-acquisition variable-resonance image combination and slice encoding for metal artifact correction. Applicability of MRI in the vicinity of metal implants has been revolutionized by these new techniques at the expense of longer acquisition times. To reach clinically viable scan times, these novel techniques have been successfully coupled with various acceleration paradigms such as parallel imaging and compressed sensing.
Inflammatory Conditions of the Hip
Authors: Teh James , Schueller-Weidekamm Claudia 
Published: October 2017

AbstractMost cases of adult hip pain are related to osteoarthritis, so inflammatory causes are often not considered. This review article describes the different entities of hip inflammation and provides an approach on how to differentiate rheumatologic disorders from degenerative changes, synovial tumors, and infection. Indications for imaging, the correct choice of imaging modality, and scanning protocols are also discussed. The differential diagnosis of inflammatory lesions of the hips is addressed in the context of image interpretation.
Bilateral Traumatic Thalamic Hemorrhage: A Rare Clinical Presentation
Authors: Mandour Cherkaoui , Gazzaz Miloudi , Mostarchid Brahim 
Published: October 2017

AbstractBilateral traumatic thalamic hemorrhage is a very rare occurrence, especially after head trauma, and is limited to case reports. The authors present a 27-year-old man, admitted for head trauma causing bilateral thalamic bleeding. Posttraumatic intracerebral bleeding is caused by focal or diffuse axonal injury. Bilateral traumatic thalamic hemorrhage is a rare clinical and radiologic presentation.
Multifokale Elektroretinografie bei Patienten mit Poppers-Makulopathie
Authors: Pahlitzsch Milena , Salchow Daniel , Rossel Mirjam , Bergholz Richard 
Published: October 2017

Zusammenfassung
Hintergrund Die Makulopathie ist eine potenzielle Nebenwirkung der inhalativen Amylnitrit- bzw. Poppers-Einnahme. Sie ist gekennzeichnet durch einen plötzlichen, schmerzlosen und i. d. R. bilateralen Visusverlust. Während die funduskopischen Veränderungen dezent sind, zeigen sich im OCT typische Alterationen der subfovealen, äußeren Netzhautschichten. Das genaue Ausmaß der retinalen Funktionsbeeinträchtigung ist bislang unzureichend charakterisiert.
Material/Methoden Wir verglichen die Antwortdichten und Gipfelzeiten von N1 und P1 im multifokalen Elektroretinogramm (mfERG) von 6 Patienten mit Poppers-Makulopathie (12 Augen, medianes Alter 46 Jahre) mit denen einer Kontrollgruppe bestehend aus 6 gesunden Probanden (12 Augen, medianes Alter 45,5 Jahre).
Ergebnisse Sowohl die Antwortdichten als auch die Gipfelzeiten von N1 und P1 waren bei den Patienten mit Poppers-Makulopathie niedriger. Dies galt vor allem für das Zentralpotenzial (Ring 1) und die peripheren Ringe 4 und 5. Ein statistisch signifikanter Unterschied ergab sich aber lediglich für ein Hexagon der N1-Antwortdichte (p = 0,039), nicht aber für die Summenantwort oder die gemittelten Exzentrizitäten (Ring 1 – 5, p > 0,05).
Schlussfolgerung Das multifokale Elektroretinogramm ist bei Patienten mit Poppers-Makulopathie nicht relevant beeinträchtigt, was im Kontrast zur deutlichen Auswirkung auf die Sehschärfe steht. Das multifokale Elektroretinogramm eignet sich im Gegensatz zum OCT nicht zur Diagnosestellung.
Einfluss der Makulareifung auf Ganzfeld- und multifokales ERG bei ehemaligen Extremfrühgeborenen mit und ohne akute Frühgeborenenretinopathie
Authors: Bowl Wadim , Schweinfurth Silke , Holve Kerstin , Stieger Knut , Lorenz Birgit , Andrassi-Darida Monika 
Published: October 2017

Zusammenfassung
Einleitung Die Entwicklung und Reifung der Netzhaut ist ein komplexer Prozess, der sich bis in das Kleinkindesalter und darüber hinaus erstrecken kann. Die zentrale Netzhaut frühgeborener Kinder kann durch eine Reifungsstörung betroffen sein, die durch eine abgeflachte, dickere Fovea mit überlagernden Schichten innerer Netzhaut und einem gestörten Verhältnis der äußeren Photorezeptorschicht zu den inneren Netzhautschichten („macular developmental arrest“: MDA) gekennzeichnet ist und zu funktionellen Nachteilen führen kann (Bowl et al. 2016 18). In dieser Studie untersuchten wir ehemalige Extremfrühgeborene mit spontan zurückgebildeter Frühgeborenenretinopathie (ROP) und ohne ROP sowie gleichaltrige Termingeborene elektrophysiologisch und betrachteten die Ergebnisse in Bezug zur Makulareifung.
Methoden Im Rahmen einer umfangreichen prospektiven Kohortenstudie untersuchten wir n = 60 frühgeborene Kinder mit spontan rückgebildeter ROP (srROP, n = 15), ohne ROP (noROP, n = 45) in der Anamnese sowie n = 10 termingeborene altersentsprechende Kinder (Term). Bei jedem Kind wurden zur funktionellen Analyse jeweils ein Ganzfeldelektroretinogramm (ffERG: a- und b-Welle), multifokales ERG (mfERG: P1) nach ISCEV-Standard sowie eine optische Kohärenztomografie (SD-OCT, Spectralis, Heidelberg Engineering, Deutschland) der Fovea zur morphologischen Einordnung durchgeführt.
Ergebnisse Im skotopischen ffERG zeigten Kinder mit srROP und noROP vor allem dann signifikant reduzierte b-Wellen-Antworten, wenn ein MDA im OCT nachweisbar war. Im mfERG war die P1-Komponente des zentralen Hexagons und des 2. konzentrischen Ringes bei Kindern mit MDA signifikant reduziert. Alle anderen Parameter zeigten keinen signifikanten Unterschied zwischen den Gruppen.
Schlussfolgerung Elektrophysiologische Veränderungen finden sich bei ehemaligen extrem frühgeborenen Kindern vor allem bei im SD-OCT nachweisbarer Makulareifungsstörung (MDA), und zwar sowohl bei Kindern ohne akute ROP als auch bei Kindern mit spontan zurückgebildeter ROP: Die reduzierte b-Welle im skotopischen ffERG und die reduzierte P1-Komponente im mfERG deuten auf eine Beteiligung der Bipolarzellen bei MDA hin. Insbesondere die Korrelation von MDA mit Auffälligkeiten im ffERG könnte ein Zeichen dafür sein, dass MDA mit einer globaleren Reifungsstörung der Netzhaut einhergeht und diese bei Extremfrühgeborenen mit und ohne ROP auftreten kann.
Weighted mining of massive collections of P-values by convex optimization
Authors: Dobriban Edgar 
Published: October 2017

AbstractResearchers in data-rich disciplines—think of computational genomics and observational cosmology—often wish to mine large bodies of $P$-values looking for significant effects, while controlling the false discovery rate or family-wise error rate. Increasingly, researchers also wish to prioritize certain hypotheses, for example, those thought to have larger effect sizes, by upweighting, and to impose constraints on the underlying mining, such as monotonicity along a certain sequence. We introduce Princessp, a principled method for performing weighted multiple testing by constrained convex optimization. Our method elegantly allows one to prioritize certain hypotheses through upweighting and to discount others through downweighting, while constraining the underlying weights involved in the mining process. When the $P$-values derive from monotone likelihood ratio families such as the Gaussian means model, the new method allows exact solution of an important optimal weighting problem previously thought to be non-convex and computationally infeasible. Our method scales to massive data set sizes. We illustrate the applications of Princessp on a series of standard genomics data sets and offer comparisons with several previous ‘standard’ methods. Princessp offers both ease of operation and the ability to scale to extremely large problem sizes. The method is available as open-source software from github.com/dobriban/pvalue_weighting_matlab (accessed 11 October 2017).