bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intrathecal fentanyl abolishes the exaggerated blood pressure response to cycling in hypertensive men
Authors: Barbosa Thales C. , Vianna Lauro C. , Fernandes Igor A. , Prodel Eliza , Rocha Helena N. M. , Garcia Vinicius P. , Rocha Natalia G. , Secher Niels H. , Nobrega Antonio C. L. 
Published: December 2015

Key points
The increase in blood pressure observed during physical activities is exaggerated in patients with hypertension, exposing them to a higher cardiovascular risk.
Neural signals from the skeletal muscles appear to be overactive, resulting in this abnormal response in hypertensive patients.
In the present study, we tested whether the attenuation of these neural signals in hypertensive patients could normalize their abnormal increase in blood pressure during physical activity.
Attenuation of the neural signals from the leg muscles with intrathecal fentanyl injection reduced the blood pressure of hypertensive men during cycling exercise to a level comparable to that of normotensive men.
Skeletal muscle afferent overactivity causes the abnormal cardiovascular response to exercise and was reverted in this experimental model, appearing as potential target for treatment.
AbstractHypertensive patients present an exaggerated increase in blood pressure and an elevated cardiovascular risk during exercise. Although controversial, human studies suggest that group III and IV skeletal muscle afferents might contribute to this abnormal response. In the present study, we investigated whether attenuation of the group III and IV muscle afferent signal of hypertensive men eliminates the exaggerated increase in blood pressure occurring during exercise. Eight hypertensive men performed two sessions of 5 min of cycling exercise at 40 W. Between sessions, the subjects were provided with a lumbar intrathecal injection of fentanyl, a μ‐opioid receptor agonist, aiming to attenuate the central projection of opioid‐sensitive group III and IV muscle afferent nerves. The cardiovascular response to exercise of these subjects was compared with that of six normotensive men. During cycling, the hypertensive group demonstrated an exaggerated increase in blood pressure compared to the normotensive group (mean ± SEM: +17 ± 3 vs. +8 ± 1 mmHg, respectively; P < 0.05), whereas the increase in heart rate, stroke volume, cardiac output and vascular conductance was similar (P > 0.05). Fentanyl inhibited the blood pressure response to exercise in the hypertensive group (+11 ± 2 mmHg) to a level comparable to that of the normotensive group (P > 0.05). Moreover, fentanyl increased the responses of vascular conductance and stroke volume to exercise (P < 0.05), whereas the heart rate response was attenuated (P < 0.05) and the cardiac output response was maintained (P > 0.05). The results of the present study show that attenuation of the exercise pressor reflex normalizes the blood pressure response to cycling exercise in hypertensive individuals.
Creencias y actitudes hacia los medios de comunicación en estudiantes universitarios del sur de Chile
Authors: Denegri Coria Marianela , Chávez H. David , Del Valle R. Carlos , Sepúlveda A. Jocelyne 
Published: December 2015

<p>La presente investigación tiene como propósito evaluar las propiedades psicométricas de dos escalas e identificar las actitudes y creencias hacia los medios de comunicación en universitarios del sur de Chile. Se diseñó un instrumento a partir de las Escalas de Creencias hacia los Medios (ECM) y Actitudes hacia los Medios (EAM) para evaluar las creencias y actitudes hacia los medios de la población mencionada. La muestra estuvo compuesta por 187 estudiantes universitarios de la ciudad de Temuco, Chile. La escala fue sometida a un Análisis Factorial Exploratorio y quedó constituida por dos sub-escalas: la creencias acerca de los medios (ECM) y actitudes hacia los medios (EAM).</p><p> </p>
The global ocean microbiome
Authors: Moran Mary Ann 
Published: December 2015

The ocean microbial system
The vast translucent oceans are teeming with microscopic life that drives significant life processes and elemental cycling on Earth. Yet how climate change will affect the functioning of this microbiome is not well understood. Moran reviews progress and the transformative discoveries made recently in marine microbiology that have led environmental, plant, animal, and even human microbiome research.

Science
, this issue p.
10.1126/science.aac8455
Engineering Synthetic Multistress Tolerance in Escherichia coli by Using a Deinococcal Response Regulator, DR1558
Authors: Appukuttan Deepti , Singh Harinder , Park Sun-Ha , Jung Jong-Hyun , Jeong Sunwook , Seo Ho Seong , Choi Yong Jun , Lim Sangyong 
Published: December 2015

ABSTRACT

Cellular robustness is an important trait for industrial microbes, because the microbial strains are exposed to a multitude of different stresses during industrial processes, such as fermentation. Thus, engineering robustness in an organism in order to push the strains toward maximizing yield has become a significant topic of research. We introduced the deinococcal response regulator DR1558 into
Escherichia coli
(strain
Ec
-1558), thereby conferring tolerance to hydrogen peroxide (H
2
O
2
). The reactive oxygen species (ROS) level in strain
Ec
-1558 was reduced due to the increased KatE catalase activity. Among four regulators of the oxidative-stress response, OxyR, RpoS, SoxS, and Fur, we found that the expression of
rpoS
increased in
Ec
-1558, and we confirmed this increase by Western blot analysis. Electrophoretic mobility shift assays showed that DR1558 bound to the
rpoS
promoter. Because the alternative sigma factor RpoS regulates various stress resistance-related genes, we performed stress survival analysis using an
rpoS
mutant strain.
Ec
-1558 was able to tolerate a low pH, a high temperature, and high NaCl concentrations in addition to H
2
O
2
, and the multistress tolerance phenotype disappeared in the absence of
rpoS
. Microarray analysis clearly showed that a variety of stress-responsive genes that are directly or indirectly controlled by RpoS were upregulated in strain
Ec
-1558. These findings, taken together, indicate that the multistress tolerance conferred by DR1558 is likely routed through RpoS. In the present study, we propose a novel strategy of employing an exogenous response regulator from polyextremophiles for strain improvement.
Acquisition and Longevity of Antibodies to Plasmodium vivax Preerythrocytic Antigens in Western Thailand
Authors: Longley Rhea J. , Reyes-Sandoval Arturo , Montoya-Díaz Eduardo , Dunachie Susanna , Kumpitak Chalermpon , Nguitragool Wang , Mueller Ivo , Sattabongkot Jetsumon 
Published: December 2015

ABSTRACTPlasmodium vivaxis now the dominantPlasmodiumspecies causing malaria in Thailand, yet little is known about naturally acquired immune responses to this parasite in this low-transmission region. The preerythrocytic stage of theP. vivaxlife cycle is considered an excellent target for a malaria vaccine, and in this study, we assessed the stability of the seropositivity and the magnitude of IgG responses to three different preerythrocyticP. vivaxproteins in two groups of adults from a region of western Thailand where malaria is endemic. These individuals were enrolled in a yearlong cohort study, which comprised one group that remainedP. vivaxfree (by quantitative PCR [qPCR] detection,n= 31) and another that experienced two or more blood-stageP. vivaxinfections during the year of follow up (n= 31). Despite overall low levels of seropositivity, IgG positivity and magnitude were long-lived over the 1-year period in the absence of qPCR-detectable blood-stageP. vivaxinfections. In contrast, in the adults with two or moreP. vivaxinfections during the year, IgG positivity was maintained, but the magnitude of the response toP. vivaxcircumsporozoite protein 210 (CSP210) decreased over time. These findings demonstrate that long-term humoral immunity can develop in low-transmission regions.
Genome Sequence of the Mosquitocidal Bacillus thuringiensis Strain BR58, a Biopesticide Product Effective against the Coffee Berry Borer (
Hypothenemus hampei
)
Authors: Zorzetti Janaina , Ricietto Ana P. S. , da Silva Carlos R. M. , Wolf Ivan R. , Vilas-Bôas Gislayne T. , Neves Pedro M. O. J. , Meneguim Ana M. , Vilas-Boas Laurival A. 
Published: December 2015

ABSTRACT

Bacillus thuringiensis
is an important microbial control agent against insect pests. The draft genome sequence of the Brazilian strain BR58 described here contains the insecticidal genes
cry4A
,
cry4B
,
cry10A
,
cry11A
,
cry60A
,
cry60B
, and
cyt1A
, which show toxicity to both
Aedes aegypti
and
Hypothenemus hampei
larvae.
Genome Sequences of Three
Pseudoalteromonas
Strains (P1-8, P1-11, and P1-30), Isolated from the Marine Hydroid
Hydractinia echinata
Authors: Klassen Jonathan L. , Rischer Maja , Wolf Thomas , Guo Huijuan , Shelest Ekaterina , Clardy Jon , Beemelmanns Christine 
Published: December 2015

ABSTRACT

The genomes of three
Pseudoalteromonas
strains (P1-8, P1-11, and P1-30) were sequenced and assembled. These genomes will inform future study of the genes responsible for the production of biologically active compounds responsible for these strains’ antimicrobial, biofouling, and algicidal activities.
Whole-Genome Sequence of a European Clone II and OXA-72-Producing Acinetobacter baumannii Strain from Serbia
Authors: Dortet Laurent , Bonnin Rémy A. , Girlich Delphine , Imanci Dilek , Bernabeu Sandrine , Fortineau Nicolas , Naas Thierry 
Published: December 2015

ABSTRACT

We report here the draft genome sequence of a carbapenem-resistant
Acinetobacter baumannii
strain isolated from a patient, a strain which previously stayed in Serbia. This isolate possessed the
bla
OXA-72
carbapenemase gene. The draft genome sequence consists of a total length of 3.91 Mbp, with an average G+C content of 38.8%.
Complete Genome Sequence of a Polypropylene Glycol-Degrading Strain,
Microbacterium
sp. No. 7
Authors: Ohtsubo Yoshiyuki , Nagata Yuji , Numata Mitsuru , Tsuchikane Kieko , Hosoyama Akira , Yamazoe Atsushi , Tsuda Masataka , Fujita Nobuyuki , Kawai Fusako 
Published: December 2015

ABSTRACT

Microbacterium
(formerly
Corynebacterium
) sp. No. 7 was isolated from activated sludge as a polypropylene glycol (PPG)-assimilating bacterial strain. Its oxidative PPG degradation has been proposed on the basis of PPG dehydrogenase activity and the metabolic products. Here, we report the complete genome sequence of
Microbacterium
sp. No. 7. The genome of the strain No. 7 is composed of a 4,599,046-bp circular chromosome and two linear plasmids. The whole finishing was conducted
in silico
with aids of the computational tools GenoFinisher and AceFileViewer. Strain No. 7 is available from the Biological Resource Center, National Institute of Technology and Evaluation (NITE) (Tokyo, Japan).
Complete Genome Sequence of Sphingopyxis macrogoltabida Type Strain NBRC 15033, Originally Isolated as a Polyethylene Glycol Degrader
Authors: Ohtsubo Yoshiyuki , Nagata Yuji , Numata Mitsuru , Tsuchikane Kieko , Hosoyama Akira , Yamazoe Atsushi , Tsuda Masataka , Fujita Nobuyuki , Kawai Fusako 
Published: December 2015

ABSTRACT

Sphingopyxis macrogoltabida
strain 203, the type strain of the species, grew on polyethylene glycol (PEG) and has been deposited to the stock culture at the Biological Resource Center, National Institute of Technology and Evaluation (NITE), under the number NBRC 15033. Here, we report the complete genome sequence of strain NBRC 15033. Unfortunately, genes for PEG degradation were missing.
Draft Genome Sequence of the Hydrocarbon-Degrading Bacterium Alcanivorax dieselolei KS-293 Isolated from Surface Seawater in the Eastern Mediterranean Sea
Authors: Barbato Marta , Mapelli Francesca , Chouaia Bessem , Crotti Elena , Daffonchio Daniele , Borin Sara 
Published: December 2015

ABSTRACT

We report here the draft genome sequence of
Alcanivorax dieselolei
KS-293, a hydrocarbonoclastic bacterium isolated from the Mediterranean Sea, by supplying diesel oil as the sole carbon source. This strain contains multiple putative genes associated with hydrocarbon degradation pathways and that are highly similar to those described in
A. dieselolei
type strain B5.
Draft Genome Sequence of
Streptomyces
sp. TP-A0871, a Producer of Heronamide C
Authors: Komaki Hisayuki , Ichikawa Natsuko , Hosoyama Akira , Fujita Nobuyuki , Igarashi Yasuhiro 
Published: December 2015

ABSTRACT

Streptomyces
sp. TP-A0871 produces the polyene macrolactam heronamide C. Here, we report its draft genome sequence to get insight into heronamide biosynthesis and genome-mining for novel secondary metabolites of polyketide and nonribosomal peptide classes. The genome encodes over nine orphan gene clusters for polyketide and/or nonribosomal peptide syntheses.
Draft Genome Sequence of
Nonomuraea
sp. TP-A0861, a Producer of Myxochelin A
Authors: Komaki Hisayuki , Ichikawa Natsuko , Hosoyama Akira , Fujita Nobuyuki , Igarashi Yasuhiro 
Published: December 2015

ABSTRACT

Nonomuraea
sp. TP-A0861 produces the nonribosomal peptide myxochelin A, which is known as a microbial siderophore. Here, we report its draft genome sequence. The genome contains at least three nonribosomal peptide synthetase gene clusters, one of which is proposed to be responsible for the biosynthesis of myxochelin A.
Draft Genome Sequence of
Streptomyces
sp. TP-A0356, a Producer of Yatakemycin
Authors: Komaki Hisayuki , Ichikawa Natsuko , Hosoyama Akira , Fujita Nobuyuki , Igarashi Yasuhiro 
Published: December 2015

ABSTRACT

Here, we report the draft genome sequence of
Streptomyces
sp. TP-A0356, a producer of a potent antitumor antibiotic, yatakemycin, to evaluate potential for secondary metabolite production. The genome sequence data suggest the presence of at least nine gene clusters for polyketide synthases and nonribosomal peptide synthetases in this strain.
Complete Genome Sequence of Pseudomonas aeruginosa PA1, Isolated from a Patient with a Respiratory Tract Infection
Authors: Lu Shuguang , Le Shuai , Li Gang , Shen Mengyu , Tan Yinling , Zhao Xia , Wang Jing , Shen Wei , Guo Keke , Yang Yuhui , Zhu Hongbin , Li Shu , Li Ming , Zhu Junmin , Rao Xiancai , Hu Fuquan 
Published: December 2015

ABSTRACT

We report the 6,498,072-bp complete genome sequence of
Pseudomonas aeruginosa
PA1, which was isolated from a patient with a respiratory tract infection in Chongqing, People's Republic of China. Whole-genome sequencing was performed using single-molecule real-time (SMRT) technology, and
de novo
assembly revealed a single contig with 396-fold sequence coverage.
Draft Genome Sequence of Mycobacterium tuberculosis Clinical Strain W06, a Prevalent Beijing Genotype Isolated in Taiwan
Authors: Liao Yu-Chieh , Liu Tze-Tze , Chang Jia-Ru , Chen Yih-Yuan , Lin Hsin-Hung , Hsu Chih-Hao , Lin Ching-Yen , Lo Ying-Chih , Dou Horng-Yunn 
Published: December 2015

ABSTRACT

Mycobacterium tuberculosis
strain W06, analyzed by molecular methods, was classified as a modern Beijing
M. tuberculosis
strain, the most predominant strain in Taiwan. To our knowledge, this is the first draft genome announcement of a Beijing
M. tuberculosis
strain in Taiwan.
Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila strain KOR1, Isolated from a Mangrove Plant (
Kandelia obovata
)
Authors: Yin Mengqing , Ma Zhiping , Cai Zhonghua , Lin Guanghui , Zhou Jin 
Published: December 2015

ABSTRACT

Aeromonas hydrophila
strain KOR1, isolated from mangrove rhizosphere soil, has the ability to produce the quorum-sensing signal molecule. Here, we report the 4.78-Mb genome sequence of strain KOR1, and found its quorum-sensing encoding gene
LuxR
. The data will be crucial to understanding the quorum-sensing-dependent phenotypes of this bacterium.
Complete Genome Sequences for Two Strains of a Novel Fastidious, Partially Acid-Fast, Gram-Positive
Corynebacterineae
Bacterium, Derived from Human Clinical Samples
Authors: Nicholson Ainsley C. , Bell Melissa , Humrighouse Ben W. , McQuiston John R. 
Published: December 2015

ABSTRACT

Here we report the complete genome sequences of two strains of the novel fastidious, partially acid-fast, Gram-positive bacillus “
Lawsonella clevelandensis
” (proposed). Their clinical relevance and unusual growth characteristics make them intriguing candidates for whole-genome sequencing.
Draft Genome Sequence of Bifidobacterium aesculapii DSM 26737
T
, Isolated from Feces of Baby Common Marmoset
Authors: Toh Hidehiro , Yamazaki Yumiko , Tashiro Kosuke , Kawarai Shinpei , Oshima Kenshiro , Nakano Akiyo , Kim Co Nguyen Thi , Mimura Iyo , Arakawa Kensuke , Iriki Atsushi , Kikusui Takefumi , Morita Hidetoshi 
Published: December 2015

ABSTRACT

Bifidobacterium aesculapii
DSM 26737
T
was isolated from feces of baby common marmoset. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of
B. aesculapii
.
Draft Genome Sequence of Linfuranone Producer
Microbispora
sp. GMKU 363
Authors: Komaki Hisayuki , Ichikawa Natsuko , Hosoyama Akira , Fujita Nobuyuki , Thamchaipenet Arinthip , Igarashi Yasuhiro 
Published: December 2015

ABSTRACT

Here, we report the draft genome sequence of
Microbispora
sp. GMKU 363, a plant-derived actinomycete that produces linfuranone A, a linear polyketide modified with a furanone ring possessing adipocyte differentiation inducing activity. The biosynthetic gene cluster for linfuranone was identified by analyzing polyketide synthase genes in the genome.
Body mass index and risk of knee osteoarthritis: systematic review and meta-analysis of prospective studies
Authors: Zheng Huaqing , Chen Changhong 
Published: December 2015

ObjectivesObesity is suggested to be a risk factor for knee osteoarthritis (OA). This meta-analysis aimed to examine the relationship between body mass index (BMI) and the risk of knee OA in published prospective studies.DesignMeta-analysis.Studies reviewedAn extensive literature review was performed, and relevant studies published in English were retrieved from the computerised databases MEDLINE, EMBASE and Cochrane.MethodsThe effect estimate (RR or HR) and its 95% CI are investigated on the basis of the evaluation of differences of knee OR risk in overweight or obesity versus those with normal weight. Category-specific risk estimates were further transformed into estimates of the RR in terms of per increase of 5 in BMI by using the generalised least-squares method for trend estimation. Studies were independently reviewed by two investigators. Subgroup analysis was performed. Heterogeneity and publication bias were assessed. Data from eligible studies were extracted, and the meta-analysis was performed by using the STATA software V.12.0.Results14 studies were finally included in the analysis. The results showed that overweight and obesity were significantly associated with higher knee OA risks of 2.45 (95% CI 1.88 to 3.20, p<0.001) and 4.55 (95% CI 2.90 to 7.13, p<0.001), respectively. The risk of knee OA increases by 35% (95% CI 1.18 to 1.53, p<0.001) with a 5 kg/m2increase in BMI. Subgroup analysis showed that obesity was an independent predictor of knee OA risk regardless of the study country, sample size, gender proportion of participants, duration of follow-up, presence of adjusted knee injury and assessed study quality above or below an NOS score of 8. No publication bias was detected.ConclusionsObesity was a robust risk factor for knee OA. Professionals should take a possible weight reduction into account for the treatment of knee OA whenever a patient is significantly overweight.
High prevalence of HIV among men who have sex with men in Zhejiang, China: a respondent-driven sampling survey
Authors: Pan Xiaohong , Wu Minni , Ma Qiaoqin , Wang Hui , Ma Wenzhe , Zeng Shidian , Chen Junfang , Zhang Yan , Miao Dandan , Zhou Xin , Jiang Tingting , He Lin , Xia Yan , Peng Zhihang , Xia Shichang 
Published: December 2015

ObjectivesTo examine the prevalence of HIV and risk behaviours among men who have sex with men (MSM) and to explore the feasibility of using respondent-driven sampling in this population in order to conduct customised interventions among MSM in the future.DesignCross-sectional study.SettingZhejiang, China.Participants1316 MSM.Primary and secondary outcome measuresHIV prevalence rates and factors associated with HIV infection; sociodemographic and behavioural information of participants, syphilis prevalence rates.ResultsThe adjusted prevalence of HIV and syphilis were 13.8% and 11.4%, respectively. Multivariate analysis showed that higher educational level, support treatment of HIV, negative syphilis are protective factors of HIV infection. MSM who had heterosexual behaviour before and whose primary sexual partner was HIV-positive were less likely to be infected with HIV compared with their counterparts, while frequency of sexual behaviour with primary sexual partner was positively associated with HIV infection.ConclusionsThis survey confirmed a high HIV prevalence among MSM in Zhejiang province. MSM are extremely vulnerable to HIV infection and comprehensive interventions are urgently needed to slow the spread of HIV among MSM.
Adding point of care ultrasound to assess volume status in heart failure patients in a nurse-led outpatient clinic. A randomised study
Authors: Gundersen Guri Holmen , Norekval Tone M , Haug Hilde Haugberg , Skjetne Kyrre , Kleinau Jens Olaf , Graven Torbjorn , Dalen Havard 
Published: December 2015

ObjectivesMedical history, physical examination and laboratory testing are not optimal for the assessment of volume status in heart failure (HF) patients. We aimed to study the clinical influence of focused ultrasound of the pleural cavities and inferior vena cava (IVC) performed by specialised nurses to assess volume status in HF patients at an outpatient clinic.MethodsHF outpatients were prospectively included and underwent laboratory testing, history recording and clinical examination by two nurses with and without an ultrasound examination of the pleural cavities and IVC using a pocket-size imaging device, in random order. Each nurse worked in a team with a cardiologist. The influence of the different diagnostic tests on diuretic dosing was assessed descriptively and in linear regression analyses.ResultsSixty-two patients were included and 119 examinations were performed. Mean±SD age was 74±12 years, EF was 34±14%, and N-terminal pro-brain natriuretic peptide (NT-proBNP) value was 3761±3072 ng/L. Dosing of diuretics differed between the teams in 31 out of 119 consultations. Weight change and volume status assessed clinically with and without ultrasound predicted dose adjustment of diuretics at follow-up (p<0.05). Change of oedema, NT-proBNP, creatinine, and symptoms did not (p≥0.10). In adjusted analyses, only volume status based on ultrasound predicted dose adjustments of diuretics at first visit and follow-up (all ultrasound p≤0.01, all other p≥0.2).ConclusionsUltrasound examinations of the pleural cavities and IVC by nurses may improve diagnostics and patient care in HF patients at an outpatient clinic, but more studies are needed to determine whether these examinations have an impact on clinical outcomes.Trial registration numberNCT01794715.