bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of Current Psychiatric Disorders
Authors: Oude Engberink Agnés , Carbonnel François , David Michel , Norton Joanna , Bourrel Gérard , Boulenger Jean-Philippe , Capdevielle Delphine 
Published: April 2016

Objective: Describe and analyse the experience of family physicians in managing current psychiatric disorders to obtain a better understanding of the underlying reasons of under-detection and inadequate prescribing identified in studies. Methods: A qualitative study using in-depth interviews. Sample of 15 practicing family physicians, recruited by telephone from a precedent cohort (Sesame1) with a maximum variation: sex, age, single or group practice, urban or rural. Qualitative method is inspired by the completed grounded theory of a verbatim semiopragmatic analysis from 2 experts in this approach. Results: Family physicians found that current psychiatric disorders were related to psychological symptoms in reaction to life events. Their role was to make patients aware of a psychiatric symptom rather than establish a diagnosis. Their management responsibility was considered in contrasting ways: it was claimed or endured. They defined their position as facilitating compliance to psychiatrist consultations, while assuring a complementary psychotherapeutic approach. Prescribing medication was not a priority for them. Conclusions: The identified under-detection is essentially due to inherent frontline conditions and complexity of clinical forms. The family physician role, facilitating compliance to psychiatrist consultations while assuring a support psychotherapy is the main result of this study. More studies should be conducted to define more accurately the clinical reality, management and course of current psychiatric disorders in primary care.
Entrapment of the Superficial Peroneal Nerve Following a Distal Fibula Fracture
Authors: Corey Robert M. , Salazar Dane H. 
Published: April 2016

Although peripheral nerve injury may result from fractures involving the long bones, bony entrapment of peripheral nerves is infrequently encountered. This report demonstrates a rare case of superficial peroneal nerve entrapment between 2 fracture ends of the distal fibula following a closed ankle fracture resulting from a supination-external rotation mechanism. Levels of Evidence: Therapeutic, Level IV: Case report
Expression and functional analysis of the Wnt/beta-catenin induced mir-135a-2 locus in embryonic forebrain development
Authors: Caronia-Brown Giuliana , Anderegg Angela , Awatramani Rajeshwar 
Published: April 2016

Abstract

Background
Brain size and patterning are dependent on dosage-sensitive morphogen signaling pathways – yet how these pathways are calibrated remains enigmatic. Recent studies point to a new role for microRNAs in tempering the spatio-temporal range of morphogen functions during development. Here, we investigated the role of miR-135a, derived from the mir-135a-2 locus, in embryonic forebrain development.

Method
1. We characterized the expression of miR-135a, and its host gene Rmst, by in situ hybridization (ish). 2. We conditionally ablated, or activated, beta-catenin in the dorsal forebrain to determine if this pathway was necessary and/or sufficient for Rmst/miR-135a expression. 3. We performed bioinformatics analysis to unveil the most predicted pathways targeted by miR-135a. 4. We performed gain and loss of function experiments on mir-135a-2 and analyzed by ish the expression of key markers of cortical hem, choroid plexus, neocortex and hippocampus.

Results
1. miR-135a, embedded in the host long non-coding transcript Rmst, is robustly expressed, and functional, in the medial wall of the embryonic dorsal forebrain, a Wnt and TGFβ/BMP-rich domain. 2. Canonical Wnt/beta-catenin signaling is critical for the expression of Rmst and miR-135a, and the cortical hem determinant Lmx1a. 3. Bioinformatics analyses reveal that the Wnt and TGFβ/BMP cascades are among the top predicted pathways targeted by miR-135a. 4. Analysis of mir-135a-2 null embryos showed that dorsal forebrain development appeared normal. In contrast, modest mir-135a-2 overexpression, in the early dorsal forebrain, resulted in a phenotype resembling that of mutants with Wnt and TGFβ/BMP deficits - a smaller cortical hem and hippocampus primordium associated with a shorter neocortex as well as a less convoluted choroid plexus. Interestingly, late overexpression of mir-135a-2 revealed no change.

Conclusions
All together, our data suggests the existence of a Wnt/miR-135a auto-regulatory loop, which could serve to limit the extent, the duration and/or intensity of the Wnt and, possibly, the TGFβ/BMP pathways.
A note on the paper ‘Fixed point theorems for cyclic weak contractions in compact metric spaces’
Authors: Kadelburg Zoran , Radenović Stojan , Vujaković Jelena 
Published: April 2016

AbstractWe show that the result on cyclic weak contractions of Harjani et al. (J. Nonlinear Sci. Appl. 6:279-284, 2013) holds without the assumption of compactness of the underlying space, and also without the assumption of continuity of the given mapping.
Cancer and Fertility Program Improves Patient Satisfaction With Information Received
Authors: Kelvin Joanne F. , Thom Bridgette , Benedict Catherine , Carter Jeanne , Corcoran Stacie , Dickler Maura N. , Goodman Karyn A. , Margolies Allison , Matasar Matthew J. , Noy Ariela , Goldfarb Shari B. 
Published: April 2016

Purpose A cancer and fertility program was established at a large cancer center to support clinicians in discussing treatment-related fertility risks and fertility preservation (FP) options with patients and in referring patients to reproductive specialists. The program provides resources, clinician education, and fertility clinical nurse specialist consultation. This study evaluated the program’s impact on patient satisfaction with information received. Patients and Methods Retrospective cross-sectional surveys assessed satisfaction before (cohort 1 [C1]) and after (cohort 2 [C2]) program initiation. Questionnaires were investigator-designed, gender-specific, and anonymous. Results Most C1 (150 males, 271 females) and C2 (120 males, 320 females) respondents were 2 years postdiagnosis; the most frequently reported cancers were testicular, breast, and lymphoma. A significant difference in satisfaction with the amount of information received was seen between C1 and C2. For males, satisfaction with information on fertility risks was high in both cohorts but significantly greater in C2 for information on sperm banking (χ2 = 9.3, P = .01) and finding a sperm bank (χ2 = 13.3, P = .001). For females, satisfaction with information was significantly greater in C2 for information on fertility risks (χ2 = 62.1, P < .001), FP options (χ2 = 71.9, P < .001), help with decision making (χ2 = 80.2, P < .001), and finding a reproductive endocrinologist (χ2 = 60.5, P < .001). Among patients who received and read information materials, 96% of males and 99% of females found them helpful. Among C2 females, fertility clinical nurse specialist consultation was associated with significantly greater satisfaction with information on FP options (χ2 = 11.2, P = .004), help with decision making (χ2 = 10.4, P = .006), and finding a reproductive endocrinologist (χ2 = 22.6, P < .001), with 10% reporting lack of knowledge as a reason for not pursuing FP. Conclusion Improvements in patient satisfaction with information received demonstrate the potential for fertility programs in cancer care settings to improve the quality of clinician-patient discussions about fertility.
A Randomized Phase II Trial of Short-Course Androgen Deprivation Therapy With or Without Bevacizumab for Patients With Recurrent Prostate Cancer After Definitive Local Therapy
Authors: McKay Rana R. , Zurita Amado J. , Werner Lillian , Bruce Justine Y. , Carducci Michael A. , Stein Mark N. , Heath Elisabeth I. , Hussain Arif , Tran Hai T. , Sweeney Christopher J. , Ross Robert W. , Kantoff Philip W. , Slovin Susan F. , Taplin Mary-Ellen 
Published: April 2016

Purpose Patients with recurrent prostate cancer after local treatment make up a heterogeneous population for whom androgen deprivation therapy (ADT) is the usual treatment. The purpose of this randomized phase II trial was to investigate the efficacy and toxicity of short-course ADT with or without bevacizumab in men with hormone-sensitive prostate cancer. Patients and Methods Eligible patients had an increasing prostate-specific antigen (PSA) of ≤ 50 ng/mL and PSA doubling time of less than 18 months. Patients had either no metastases or low burden, asymptomatic metastases (lymph nodes < 3 cm and five or fewer bone metastases). Patients were randomly assigned 2:1 to a luteinizing hormone-releasing hormone agonist, bicalutamide and bevacizumab or ADT alone, for 6 months. The primary end point was PSA relapse-free survival (RFS). Relapse was defined as a PSA of more than 0.2 ng/mL for prostatectomy patients or PSA of more than 2.0 ng/mL for primary radiation therapy patients. Results Sixty-six patients received ADT + bevacizumab and 36 received ADT alone. Patients receiving ADT + bevacizumab had a statistically significant improvement in RFS compared with patients treated with ADT alone (13.3 months for ADT + bevacizumab v 10.2 months for ADT alone; hazard ratio, 0.47; 95% CI, 0.29 to 0.77; log-rank P = .002). Hypertension was the most common adverse event in patients receiving ADT + bevacizumab (36%). Conclusion ADT combined with bevacizumab resulted in an improved RFS for patients with hormone-sensitive prostate cancer. Long-term follow-up is needed to determine whether some patients have a durable PSA response and are able to remain off ADT for prolonged periods. Our data provide rationale for combining vascular endothelial growth factor–targeting therapy with ADT in hormone-sensitive prostate cancer.
Prepregnancy Adverse Lipid Profile and Subsequent Risk of Gestational Diabetes
Authors: Han Emily S. , Krauss Ronald M. , Xu Fei , Sridhar Sneha B. , Ferrara Assiamira , Quesenberry Charles P. , Hedderson Monique M. 
Published: April 2016

Context:Lower low-density lipoprotein (LDL) peak diameter and a predominance of small, dense LDL are associated with type 2 diabetes, but it is unclear whether they are a risk factor for gestational diabetes mellitus (GDM).Objective:To evaluate whether prepregnancy lipid profile predicts the development of GDM during pregnancy.Design:A nested case-control study among women who participated in a multiphasic health exam, where blood was collected and stored between 1984 and 1996, and who then had a subsequent pregnancy between 1984 and 2009.Setting:Kaiser Permanente Northern California.Participants:Cases were 254 women who developed GDM. Two controls were selected for each case and matched for year of blood draw, age at baseline, age at pregnancy, and number of intervening pregnancies.Main Outcome Measures:Prepregnancy LDL peak diameter and prepregnancy lipid subfraction concentrations grouped according to size, and the odds of developing GDM.Results:Women in the lowest quartiles of LDL peak diameter and high-density lipoprotein had increased odds of GDM compared with women in the highest quartiles (odds ratio [95% CI], 2.60 [1.37–4.94] and 1.98 [1.01–3.86], respectively), in multivariable adjusted models. Being in the highest quartile of small and very small LDL subfractions also increased the odds of GDM (2.61 [1.35–5.03] and 2.44 [1.22–4.85], respectively).Conclusions:Lower LDL peak diameter size and high-density lipoprotein levels and higher levels of small and very small LDL subfraction groups were present years before pregnancy in women who developed GDM. A prepregnancy atherogenic lipid profile may help identify women at risk of GDM to target for prevention.
Feliform carnivores have a distinguished constitutive innate immune response
Authors: Heinrich Sonja K. , Wachter Bettina , Aschenborn Ortwin H. K. , Thalwitzer Susanne , Melzheimer Jörg , Hofer Heribert , Czirják Gábor Á. 
Published: April 2016

ABSTRACT
Determining the immunological phenotype of endangered and threatened populations is important to identify those vulnerable to novel pathogens. Among mammals, members of the order Carnivora are particularly threatened by diseases. We therefore examined the constitutive innate immune system, the first line of protection against invading microbes, of six free-ranging carnivore species; the black-backed jackal (Canis mesomelas), the brown hyena (Hyena brunnea), the caracal (Caracal caracal), the cheetah (Acinonyx jubatus), the leopard (Panthera pardus) and the lion (Panthera leo) using a bacterial killing assay. The differences in immune responses amongst the six species were independent of their foraging behaviour, body mass or social organisation but reflected their phylogenetic relatedness. The bacterial killing capacity of black-backed jackals, a member of the suborder Caniformia, followed the pattern established for a wide variety of vertebrates. In contrast, the five representatives of the suborder Feliformia demonstrated a killing capacity at least an order of magnitude higher than any species reported previously, with a particularly high capacity in caracals and cheetahs. Our results suggest that the immunocompetence of threatened felids such as the cheetah has been underestimated and its assessment ought to consider both innate and adaptive components of the immune system.
Trim37-deficient mice recapitulate several features of the multi-organ disorder Mulibrey nanism
Authors: Kettunen Kaisa M. , Karikoski Riitta , Hämäläinen Riikka H. , Toivonen Teija T. , Antonenkov Vasily D. , Kulesskaya Natalia , Voikar Vootele , Hölttä-Vuori Maarit , Ikonen Elina , Sainio Kirsi , Jalanko Anu , Karlberg Susann , Karlberg Niklas , Lipsanen-Nyman Marita , Toppari Jorma , Jauhiainen Matti , Hiltunen J. Kalervo , Jalanko Hannu , Lehesjoki Anna-Elina 
Published: April 2016

ABSTRACT
Mulibrey nanism (MUL) is a rare autosomal recessive multi-organ disorder characterized by severe prenatal-onset growth failure, infertility, cardiopathy, risk for tumors, fatty liver, and type 2 diabetes. MUL is caused by loss-of-function mutations in TRIM37, which encodes an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family and having both peroxisomal and nuclear localization. We describe a congenic Trim37 knock-out mouse (Trim37−/−) model for MUL. Trim37−/− mice were viable and had normal weight development until approximately 12 months of age, after which they started to manifest increasing problems in wellbeing and weight loss. Assessment of skeletal parameters with computer tomography revealed significantly smaller skull size, but no difference in the lengths of long bones in Trim37−/− mice as compared with wild-type. Both male and female Trim37−/− mice were infertile, the gonads showing germ cell aplasia, hilus and Leydig cell hyperplasia and accumulation of lipids in and around Leydig cells. Male Trim37−/− mice had elevated levels of follicle-stimulating and luteinizing hormones, but maintained normal levels of testosterone. Six-month-old Trim37−/− mice had elevated fasting blood glucose and low fasting serum insulin levels. At 1.5 years Trim37−/− mice showed non-compaction cardiomyopathy, hepatomegaly, fatty liver and various tumors. The amount and morphology of liver peroxisomes seemed normal in Trim37−/− mice. The most consistently seen phenotypes in Trim37−/− mice were infertility and the associated hormonal findings, whereas there was more variability in the other phenotypes observed. Trim37−/− mice recapitulate several features of the human MUL disease and thus provide a good model to study disease pathogenesis related to TRIM37 deficiency, including infertility, non-alcoholic fatty liver disease, cardiomyopathy and tumorigenesis.
Vimentin filaments regulate integrin–ligand interactions by binding to the cytoplasmic tail of integrin β3
Authors: Kim Jiyoon , Yang Chansik , Kim Eun Jin , Jang Jungim , Kim Se-Jong , Kang So Min , Kim Moon Gyo , Jung Hosung , Park Dongeun , Kim Chungho 
Published: April 2016

ABSTRACT
Vimentin, an intermediate filament protein induced during epithelial-to-mesenchymal transition, is known to regulate cell migration and invasion. However, it is still unclear how vimentin controls such behaviors. In this study, we aimed to find a new integrin regulator by investigating the H-Ras-mediated integrin suppression mechanism. Through a proteomic screen using the integrin β3 cytoplasmic tail protein, we found that vimentin might work as an effector of H-Ras signaling. H-Ras converted filamentous vimentin into aggregates near the nucleus, where no integrin binding can occur. In addition, an increase in the amount of vimentin filaments accessible to the integrin β3 tail enhanced talin-induced integrin binding to its ligands by inducing integrin clustering. In contrast, the vimentin head domain, which was found to bind directly to the integrin β3 tail and compete with endogenous vimentin filaments for integrin binding, induced nuclear accumulation of vimentin filaments and reduced the amount of integrin–ligand binding. Finally, we found that expression of the vimentin head domain can reduce cell migration and metastasis. From these data, we suggest that filamentous vimentin underneath the plasma membrane is involved in increasing integrin adhesiveness, and thus regulation of the vimentin–integrin interaction might control cell adhesion.
FRMD4A–cytohesin signaling modulates the cellular release of tau
Authors: Yan Xu , Nykänen Niko-Petteri , Brunello Cecilia A. , Haapasalo Annakaisa , Hiltunen Mikko , Uronen Riikka-Liisa , Huttunen Henri J. 
Published: April 2016

ABSTRACT
One of the defining pathological features of Alzheimer's disease is the intraneuronal accumulation of tau (also known as MAPT) protein. Tau is also secreted from neurons in response to various stimuli and accumulates in the cerebrospinal fluid of Alzheimer's disease patients. Tau pathology might spread from cell to cell through a mechanism involving secretion and uptake. Here, we developed an assay to follow cellular release and uptake of tau dimers. Individual silencing of ten common late-onset Alzheimer's disease risk genes in HEK293T cells expressing the tau reporters suggested that FRMD4A is functionally linked to tau secretion. FRMD4A depletion by using RNA interference (RNAi) reduced and overexpression increased tau secretion. The activity of cytohesins, interactors of FRMD4A and guanine-nucleotide-exchange factors of Arf6, was necessary for FRMD4A-induced tau secretion. Increased Arf6 and cell polarity signaling through Par6 and atypical protein kinase Cζ (aPKCζ) stimulated tau secretion. In mature cortical neurons, FRMD4A RNAi or inhibition of cytohesins strongly upregulated secretion of endogenous tau. These results suggest that FRMD4A, a genetic risk factor for late-onset Alzheimer's disease, regulates tau secretion by activating cytohesin–Arf6 signaling. We conclude that genetic risk factors of Alzheimer's disease might modulate disease progression by altering tau secretion.
Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls
Authors: Ma Qianqian , Sun Jingbo , Mao Tonglin 
Published: April 2016

ABSTRACT
The gaseous hormone ethylene is known to regulate plant growth under etiolated conditions (the ‘triple response’). Although organization of cortical microtubules is essential for cell elongation, the underlying mechanisms that regulate microtubule organization by hormone signaling, including ethylene, are ambiguous. In the present study, we demonstrate that ethylene signaling participates in regulation of cortical microtubule reorientation. In particular, regulation of microtubule bundling is important for this process in etiolated hypocotyls. Time-lapse analysis indicated that selective stabilization of microtubule-bundling structures formed in various arrays is related to ethylene-mediated microtubule orientation. Bundling events and bundle growth lifetimes were significantly increased in oblique and longitudinal arrays, but decreased in transverse arrays in wild-type cells in response to ethylene. However, the effects of ethylene on microtubule bundling were partially suppressed in a microtubule-bundling protein WDL5 knockout mutant (wdl5-1). This study suggests that modulation of microtubule bundles that have formed in certain orientations plays a role in reorienting microtubule arrays in response to ethylene-mediated etiolated hypocotyl cell elongation.
The Thermodynamic Stability of Adamantylideneadamantane and Its Proton- and Electron-Exchanges. Comparison with Simple Alkenes
Authors: Abboud José-Luis M , Alkorta Ibon , Dávalos Juan Z , Koppel Ilmar A , Koppel Ivar , Lenoir Dieter , Martínez Sonia , Mishima Masaaki 
Published: April 2016

Abstract
We report herein the results of an experimental and computational study of adamantylideneadamantane (1) and a variety of substituted ethylenic hydrocarbons. The standard enthalpy of formation in the gas phase as well as the gas-phase basicity (GA) of 1 were experimentally determined for the first time, respectively by calorimetric techniques and FT-ICR spectrometry. In parallel, computational studies at the MP2/6-311+G(d,p), G3(MP2), and G3 levels were performed on the neutral (1) and protonated (1H+). The agreement with experimental results was very good. The structures of 1 and 1H+ were subject to treatment by “Atoms in Molecules” in order to assess the characteristics of the closest H⋯H interactions involving both adamantane moieties. Also, the second-order perturbation analysis within the Natural Bond Orbital Theory methodology shows four degenerate charge-transfer interactions between the σ C–H bond of one of the adamantyl subunits towards the σ* C–H of the other adamantyl subunit. The standard enthalpies of formation of new adamantyl compounds were obtained using our experimental data. The computational study of a variety of ethylenic compounds including cyclohexylidenecyclohexane and several alkyl-substituted ethylenes using isodesmic and homodesmotic reactions was carried out. This study was extended to their proton affinities and gas-phase basicities.
THE GENESIS OF CLUSTER THEORY
Authors: Гуськова Надежда , Guskova Nadezhda , Скворцов Евгений , Skvortsov Evgeniy 
Published: April 2016

The article analyzes the genesis of cluster theory. Key
prerequisites for the development of the cluster theory in the historical
perspective of theories of productive forces are considered.
The key theory the basis of the cluster concept is described. A comparative
analysis of the characteristics of the various theories of
productive forces and cluster theory is made. The views of the scientific
community about the degree of novelty of the cluster theory
are summarized. Results of the analysis reveal the genesis of cluster
theory with the purpose of better understanding of the cluster concept
and long-term vision for the development of productive forces
and theories of cluster theory in particular.
REGIONAL ASPECTS OF PROBLEMS OF MODERN UNIVERSITY GRADUATES EMPLOYMENT IN NEW ECONOMIC CONDITIONS
Authors: Маликова Дарья , Malikova Darya , Борисова Наталья , Borisova Natalya 
Published: April 2016

The unemployment problem among youth is a consequence
of transition of Russia from state planned economy to the
market. University graduates face an array of problems by search of
a workplace as they are insufficiently demanded in the labour market.
Therefore it is necessary to take a number of measures for the
solution of this question, to give various help to graduates in job
search. This problem carries both social and economic nature, so
its decision will promote increase of level of living in the country
and will increase cost efficiency of Russia