bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zebrafish as a Model to Investigate Dynamin 2-Related Diseases
Authors: Bragato Cinzia , Gaudenzi Germano , Blasevich Flavia , Pavesi Giulio , Maggi Lorenzo , Giunta Michele , Cotelli Franco , Mora Marina 
Published: February 2016

AbstractMutations in the dynamin-2 gene (DNM2) cause autosomal dominant centronuclear myopathy (CNM) and dominant intermediate Charcot-Marie-Tooth (CMT) neuropathy type B (CMTDIB). As the relation between these DNM2-related diseases is poorly understood, we used zebrafish to investigate the effects of two different DNM2 mutations. First we identified a new alternatively spliced zebrafish dynamin-2a mRNA (dnm2a-v2) with greater similarity to human DNM2 than the deposited sequence. Then we knocked-down the zebrafish dnm2a, producing defects in muscle morphology. Finally, we expressed two mutated DNM2 mRNA by injecting zebrafish embryos with human mRNAs carrying the R522H mutation, causing CNM, or the G537C mutation, causing CMT. Defects arose especially in secondary motor neuron formation, with incorrect branching in embryos injected with CNM-mutated mRNA and total absence of branching in those injected with CMT-mutated mRNA. Muscle morphology in embryos injected with CMT-mutated mRNA appeared less regularly organized than in those injected with CNM-mutated mRNA. Our results showing, a continuum between CNM and CMTDIB phenotypes in zebrafish, similarly to the human conditions, confirm this animal model to be a powerful tool to investigate mutations of DNM2 in vivo.
Creep turns linear in narrow ferromagnetic nanostrips
Authors: Leliaert Jonathan , Van de Wiele Ben , Vansteenkiste Arne , Laurson Lasse , Durin Gianfranco , Dupré Luc , Van Waeyenberge Bartel 
Published: February 2016

AbstractThe motion of domain walls in magnetic materials is a typical example of a creep process, usually characterised by a stretched exponential velocity-force relation. By performing large-scale micromagnetic simulations and analyzing an extended 1D model which takes the effects of finite temperatures and material defects into account, we show that this creep scaling law breaks down in sufficiently narrow ferromagnetic strips. Our analysis of current-driven transverse domain wall motion in disordered Permalloy nanostrips reveals instead a creep regime with a linear dependence of the domain wall velocity on the applied field or current density. This originates from the essentially point-like nature of domain walls moving in narrow, line- like disordered nanostrips. An analogous linear relation is found also by analyzing existing experimental data on field-driven domain wall motion in perpendicularly magnetised media.
Exact quantum Bayesian rule for qubit measurements in circuit QED
Authors: Feng Wei , Liang Pengfei , Qin Lupei , Li Xin-Qi 
Published: February 2016

AbstractDeveloping efficient framework for quantum measurements is of essential importance to quantum science and technology. In this work, for the important superconducting circuit-QED setup, we present a rigorous and analytic solution for the effective quantum trajectory equation (QTE) after polaron transformation and converted to the form of Stratonovich calculus. We find that the solution is a generalization of the elegant quantum Bayesian approach developed in arXiv:1111.4016 by Korotokov and currently applied to circuit-QED measurements. The new result improves both the diagonal and off-diagonal elements of the qubit density matrix, via amending the distribution probabilities of the output currents and several important phase factors. Compared to numerical integration of the QTE, the resultant quantum Bayesian rule promises higher efficiency to update the measured state and allows more efficient and analytical studies for some interesting problems such as quantum weak values, past quantum state and quantum state smoothing. The method of this work opens also a new way to obtain quantum Bayesian formulas for other systems and in more complicated cases.
High production of CH4 and H2 by reducing PET waste water using a non-diaphragm-based electrochemical method
Authors: Kim Nam-Gyu , Yim Kwang-Jin , Kim Chan-Soo , Song Dong-Keun , Okuyama Kikuo , Han Min-ho , Kim Young-hoo , Lee Sung-Eun , Kim Tae-Oh 
Published: February 2016

AbstractIn recent years, the worldwide use of polyethylene terephthalate (PET) has increased exponentially. PET wastewater contains ethylene glycol (EG) and terephthalic acid (TPA). In this study, we present a unique method for producing combustible gases like CH4and H2from PET wastewater by electrochemical reaction of EG and TPA. The non-diaphragm-based electrochemical (NDE) method was used to treat PET wastewater. The electrochemical removal of EG and TPA from PET wastewater was examined and the optimal conditions for their reduction to CH4and H2were determined. Using the proposed system, 99.9% of the EG and TPA present in the PET wastewater samples were degraded to produce CH4and H2, at applied voltages lower than 5 V. The highest Faradaic efficiency achieved for EG and TPA reduction was 62.2% (CH4, 25.6%; H2, 36.6%), at an applied voltage of 0.8 V. Remarkably, CH4was produced from EG decomposition and H2from TPA decomposition. To the best of our knowledge, this is the first reported instance of CH4and H2production from EG and TPA, respectively. The electrochemical reductive treatment will be an important discovery for reducing water contamination and replacing fossil fuels with respect to generating green energy.
Hybrid magnetoresistance in Pt-based multilayers: Effect originated from strong interfacial spin-orbit coupling
Authors: Meng Kangkang , Xiao Jiaxing , Wu Yong , Miao Jun , Xu Xiaoguang , Zhao Jianhua , Jiang Yong 
Published: February 2016

AbstractThe hybrid magnetoresistance (MR) behaviors in Pt/Co90Fe10/Pt, Mn1.5Ga/Pt and Mn1.5Ga/Pt/Co90Fe10/Pt multilayers have been investigated. Both planer Hall effect (PHE) and angle-dependent MR in Pt/Co90Fe10/Pt revealed the combination of spin Hall MR (SMR) and normal anisotropic MR (AMR), indicating the large contribution of strong spin-orbit coupling (SOC) at the interfaces. When Pt contacted with perpendicular magnetic anisotropy (PMA) metal Mn1.5Ga, the strong interfacial SOC modified the effective anomalous Hall effect. The MR in Mn1.5Ga/Pt/Co90Fe10/Pt is not a simple combination of SMR and AMR, but ascribed to the complicated domain wall scattering and strong interfacial SOC when Pt is sandwiched by the in-plane magnetized Co90Fe10 and the PMA Mn1.5Ga.
Reverse-migrated neutrophils regulated by JAM-C are involved in acute pancreatitis-associated lung injury
Authors: Wu Deqing , Zeng Yue , Fan Yuting , Wu Jianghong , Mulatibieke Tunike , Ni Jianbo , Yu Ge , Wan Rong , Wang Xingpeng , Hu Guoyong 
Published: February 2016

AbstractJunctional adhesion molecule-C (JAM-C) plays a key role in the promotion of the reverse transendothelial migration (rTEM) of neutrophils, which contributes to the dissemination of systemic inflammation and to secondary organ damage. During acute pancreatitis (AP), systemic inflammatory responses lead to distant organ damage and typically result in acute lung injury (ALI). Here, we investigated the role of rTEM neutrophils in AP-associated ALI and the molecular mechanisms by which JAM-C regulates neutrophil rTEM in this disorder. In this study, rTEM neutrophils were identified in the peripheral blood both in murine model of AP and human patients with AP, which elevated with increased severity of lung injury. Pancreatic JAM-C was downregulated during murine experimental pancreatitis, whose expression levels were inversely correlated with both increased neutrophil rTEM and severity of lung injury. Knockout of JAM-C resulted in more severe lung injury and systemic inflammation. Significantly greater numbers of rTEM neutrophils were present both in the circulation and pulmonary vascular washout in JAM-C knockout mice with AP. This study demonstrates that during AP, neutrophils that are recruited to the pancreas may migrate back into the circulation and then contribute to ALI. JAM-C downregulation may contribute to AP-associated ALI via promoting neutrophil rTEM.
Cross-linguistic lexical and syntactic co-activation in L2 sentence processing
Authors: Hopp Holger 
Published: February 2016

Abstract
This study investigates under which conditions the L1 syntax is activated in L2 on-line sentence comprehension. We study whether cross-linguistic syntactic activation of the L1 word order is affected by lexical activation of the first language (L1) by virtue of cognate words. In two eye-tracking experiments, German-English bilinguals and English natives read English sentences containing reduced relative clauses whose surface word order partially overlaps with German embedded clauses. The verbs used were either German-English cognates or matched control verbs. The results show lexical cognate facilitation and syntactic co-activation of L1 word order, with the latter being moderated by proficiency and cognate status. Critically, syntactic co-activation is found only with English control words. We argue that fleeting co-activation of the L1 syntax becomes measurable under higher demands of lexical processing, while cognate facilitation frees resources for inhibition of the L1 syntax and target-like syntactic processing.
Coreference and discourse coherence in L2
Authors: Grüter Theres , Rohde Hannah , Schafer Amy J. 
Published: February 2016

Abstract
Discourse-level factors, such as event structure and the form of referential expressions, play an important role in native speakers’ referential processing. This paper presents an experiment with Japanese- and Korean-speaking learners of English, investigating the extent to which discourse-level biases that have gradient effects in L1 speakers are also implicated in L2 speakers’ coreference choices. Results from a story continuation task indicate that biases involving referential form were remarkably similar for L1 and L2 speakers. In contrast, event structure, indicated by perfective versus imperfective aspect, had a more limited effect on L2 speakers’ referential choices. The L2 results are discussed in light of existing accounts of L1 reference processing, which assume that referential choices are shaped by speakers’ continually updated expectations about what is likely to be mentioned next, and argued to reflect L2 speakers’ reduced reliance on expectations.
Discy dwarf disruption and the shape of the Galactic halo
Authors: Gibbons S. L. J. , Belokurov V. , Erkal D. , Evans N. W. 
Published: February 2016

Abstract
The shape of the Galactic dark halo can, in principle, be inferred through modelling of stellar tidal streams in the Milky Way halo. The brightest and the longest of these, the Sagittarius stream, reaches out to large Galactocentric distances and hence can deliver the tightest constraints on the Galaxy's potential. In this contribution, we revisit the idea that the Sagittarius Stream was formed from a rotating progenitor. We demonstrate that the angle between the disc's angular momentum and the progenitor's orbital angular momentum does not remain constant throughout the disruption. Instead, it undergoes a dramatic evolution caused, in part, by the changes in the progenitor's moment of inertia tensor. We show that, even in a spherical potential, the streams produced as a result of a discy dwarf disruption appear to be ‘precessing’. Yet, this debris plane evolution is illusory as it is solely caused by the swaying and wobbling of the progenitor's disc. Stream plane precession is therefore not an unambiguous indicator of asphericity of the dark halo.
A Human Factors Engineering Study of the Medication Delivery Process during an Anesthetic
Authors: Yang Yushi , Rivera Antonia Joy , Fortier Christopher R. , Abernathy James H. 
Published: February 2016

AbstractAbstract
In a work system analysis using human factors principles conducted in the operating rooms and pharmacy of a single large academic medical center, prefilled syringe were associated with simpler use and fewer system vulnerabilities compared with self-filled syringe. Use of prefilled syringe might improve the safety of perioperative medication delivery if these findings are confirmed in larger multicenter studies.

Background
Prefilled syringes (PFS) have been recommended by the Anesthesia Patient Safety Foundation. However, aspects in PFS systems compared with self-filled syringes (SFS) systems have never been explored. The aim of this study is to compare system vulnerabilities (SVs) in the two systems and understand the impact of PFS on medication safety and efficiency in the context of anesthesiology medication delivery in operating rooms.

Methods
This study is primarily qualitative research, with a quantitative portion. A work system analysis was conducted to analyze the complicated anesthesia work system using human factors principles and identify SVs. Anesthesia providers were shadowed: (1) during general surgery cases (n = 8) exclusively using SFS and (2) during general surgery cases (n = 9) using all commercially available PFS. A proactive risk assessment focus group was followed to understand the risk of each identified SV.

Results
PFS are superior to SFS in terms of the simplified work processes and the reduced number and associated risk of SVs. Eight SVs were found in the PFS system versus 21 in the SFS system. An SV example with high risk in the SFS system was a medication might need to be “drawn-up during surgery while completing other requests simultaneously.” This SV added cognitive complexity during anesthesiology medication delivery. However, it did not exist in the PFS system.

Conclusions
The inclusion of PFS into anesthesiology medication delivery has the potential to improve system safety and work efficiency. However, there were still opportunities for further improvement by addressing the remaining SVs and newly introduced complexity.
Alterations in Hair Follicle Morphology and Hair Shaft Production After Follicular Unit Transplantation
Authors: Azar Reza P. , Thomas Alexander H. , Maurer Marcus , Lindner Gerd 
Published: February 2016

Abstract:
Follicular unit transplantation is the most commonly performed technique in modern restorative hair transplantation surgery. It relies on the acquisition of intact follicular units from microdissected scalp skin strips and their subsequent transplantation into the recipient regions affected by alopecia. Ideally, the translocation of follicular units from the balding-resistant areas of the scalp (usually the occipital region) to the recipient site should not result in any morphological change in the grafts. Nevertheless, the insults associated with surgical intervention present grafted follicles to mechanical and chemical cues differently from those of the physiological steady-state conditions in undamaged skin. This disruption of the normal follicular microenvironment might alter important aspects of hair biology in grafts, for example, hair cycle and pigmentation, and, in turn, could lead to differences in hair appearance, eventually culminating in a diminished esthetical outcome of the surgery. In this study, the authors analyzed native and grafted scalp hair follicles (HFs) from 2 patients who had undergone follicular unit transplantation surgeries formerly. Scanning electron microscopy and light microscopy-based histomorphometry revealed a marked enlargement of follicular structures in the grafts with a concomitant increase in hair shaft diameter. Immunohistological staining confirmed a thickening of the dermal sheath in transplanted HFs that also harbored a denser vascular network. Taken together, these results show that the grafted HFs analyzed were subjected to marked morphological changes during their residence in the recipient site and that this phenomenon is associated with a modulation of follicular vascularization.
An open library of human kinase domain constructs for automated bacterial expression
Authors: Albanese Steven K. , Parton Daniel L. , Işık Mehtap , Rodríguez-Laureano Lucelenie , Hanson Sonya M. , Behr Julie M. , Gradia Scott , Jeans Chris , Levinson Nicholas M. , Seeliger Markus A. , Chodera John D. 
Published: February 2016

AbstractKinases play a critical role in many cellular signaling pathways and are dysregulated in a number of diseases, such as cancer, diabetes, and neurodegeneration. Since the FDA approval of imatinib in 2001, therapeutics targeting kinases now account for roughly 50% of current cancer drug discovery efforts. The ability to explore human kinase biochemistry, biophysics, and structural biology in the laboratory is essential to making rapid progress in understanding kinase regulation, designing selective inhibitors, and studying the emergence of drug resistance. While insect and mammalian expression systems are frequently used for the expression of human kinases, bacterial expression systems are superior in terms of simplicity and cost-effectiveness but have historically struggled with human kinase expression. Following the discovery that phosphatase coexpression could produce high yields of Src and Abl kinase domains in bacterial expression systems, we have generated a library of 52 His-tagged human kinase domain constructs that express above 2µg/mL culture in a simple automated bacterial expression system utilizing phosphatase coexpression (YopH for Tyr kinases, Lambda for Ser/Thr kinases). Here, we report a structural bioinformatics approach to identify kinase domain constructs previously expressed in bacteria likely to express well in a simple high-throughput protocol, experiments demonstrating our simple construct selection strategy selects constructs with good expression yields in a test of 84 potential kinase domain boundaries for Abl, and yields from a high-throughput expression screen of 96 human kinase constructs. Using a fluorescence-based thermostability assay and a fluorescent ATP-competitive inhibitor, we show that the highest-expressing kinases are folded and have well-formed ATP binding sites. We also demonstrate how the resulting expressing constructs can be used for the biophysical and biochemical study of clinical mutations by engineering a panel of 48 Src mutations and 46 Abl mutations via single-primer mutagenesis and screening the resulting library for expression yields. The wild-type kinase construct library is available publicly via Addgene, and should prove to be of high utility for experiments focused on drug discovery and the emergence of drug resistance.
PoPoolationTE2: comparative population genomics of transposable elements using Pool-Seq
Authors: Kofler Robert , Gómez-Sánchez Daniel , Schlöetterer Christian 
Published: February 2016

AbstractThe evolutionary dynamics of transposable elements (TEs) are still poorly understood. One reason is that TE abundance needs to be studied at the population level, and despite recent advances in sequencing technologies, characterizing TE abundance in multiple populations by sequencing individuals separately is still too expensive. While sequencing pools of individuals (Pool-Seq) dramatically reduces sequencing costs, a comparison of TE abundance between pooled samples has been difficult, if not impossible, due to various biases. Here, we introduce a novel bioinformatic tool, PoPoolationTE2, which is specifically tailored for the comparison of TE abundance among pooled population samples or different tissues. Using computer simulations we demonstrate that PoPoolationTE2 not only faithfully recovers TE insertion frequencies and positions but, by homogenizing the power to identify TEs acrosss samples, it provides an unbiased comparison of TE abundance between pooled population samples. We anticipate that PoPoolationTE2 will greatly facilitate the analysis of TE insertion patterns in a broad range of applications.
So much theory, so little practice: a literature review of workplace improvisation training
Authors: Ratten Vanessa , Hodge Josh 
Published: February 2016

Purpose
– The purpose of this paper is to critically review the research literature on training interventions to increase the workplace application of improvisation.

Design/methodology/approach
– A literature review was undertaken, work was considered in light of research methodology (qualitative, quantitative and discussion) and themes were identified and coded in Nvivo.

Findings
– Although there is a substantial body of research on improvisation as a workplace phenomenon, there is only limited empirical research on the workplace application of improvisation training.

Research limitations/implications
– Further research is needed in the field, specific recommendations are made.

Practical implications
– This paper provides an in-depth briefing on the current state of the literature for trainers and HR professionals who are considering the merits of using improvisation training in their workplace.

Originality/value
– Mintzberg (1973) suggested that up to 90 per cent of managerial behaviour is improvised. This paper provides a new depth of understanding on the workplace application of improvisation training and the paucity of knowledge in the field.
An empirical approach to consumer buying behavior in Indian automobile sector
Authors: Maheshwari Prateek , Seth Nitin , Gupta Anoop Kumar 
Published: February 2016

Purpose
– In today’s highly competitive business scenario, advertising symbolizes one of the key marketing activities. The purpose of this paper is to understand the relationship between advertisement effectiveness and consumer buying behavior for major automobile companies of central India.

Design/methodology/approach
– A judicious mix of qualitative (focus group and Delphi technique) and quantitative (structured questionnaire survey) research methods was used for the present study.

Findings
– Study found that for automobile sector, effective advertisements have a positive impact on consumer buying behavior. It suggests that customer’s attitude can be shaped favorably through effective advertising. Automobile companies must arrange special training sessions and learning programs for their sales force to ensure supportive customer purchasing behavior.

Research limitations/implications
– The scope of the present study is limited to major automobile companies of central India only. Although, the findings can be interpreted and generalized in a global business scenario.

Originality/value
– The research work is first of its kind that attempts to study the impact of effective advertising on consumer buying behavior in Indian automobile context. The findings and recommendations can be used for strategic planning of training sessions and workshops for different stakeholders in automobile industry.