bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-sensor integration on one microfluidics chip for single-stranded DNA detection
Authors: Yue Weiwei , Cao Yuwei , Xie Shuqi , Cheng Kang Ning , Ding Yue , Liu Cong , Ding Yan Jing , Zhu Xiaofeng , Liu Huanqing , Shafi Muhammad 
Published: September 2024

Purpose
This study aims to improve detection efficiency of fluorescence biosensor or a graphene field-effect transistor biosensor. Graphene field-effect transistor biosensing and fluorescent biosensing were integrated and combined with magnetic nanoparticles to construct a multi-sensor integrated microfluidic biochip for detecting single-stranded DNA. Multi-sensor integrated biochip demonstrated higher detection reliability for a single target and could simultaneously detect different targets.

Design/methodology/approach
In this study, the authors integrated graphene field-effect transistor biosensing and fluorescent biosensing, combined with magnetic nanoparticles, to fabricate a multi-sensor integrated microfluidic biochip for the detection of single-stranded deoxyribonucleic acid (DNA). Graphene films synthesized through chemical vapor deposition were transferred onto a glass substrate featuring two indium tin oxide electrodes, thus establishing conductive channels for the graphene field-effect transistor. Using π-π stacking, 1-pyrenebutanoic acid succinimidyl ester was immobilized onto the graphene film to serve as a medium for anchoring the probe aptamer. The fluorophore-labeled target DNA subsequently underwent hybridization with the probe aptamer, thereby forming a fluorescence detection channel.

Findings
This paper presents a novel approach using three channels of light, electricity and magnetism for the detection of single-stranded DNA, accompanied by the design of a microfluidic detection platform integrating biosensor chips. Remarkably, the detection limit achieved is 10 pm, with an impressively low relative standard deviation of 1.007%.

Originality/value
By detecting target DNA, the photo-electro-magnetic multi-sensor graphene field-effect transistor biosensor not only enhances the reliability and efficiency of detection but also exhibits additional advantages such as compact size, affordability, portability and straightforward automation. Real-time display of detection outcomes on the host facilitates a deeper comprehension of biochemical reaction dynamics. Moreover, besides detecting the same target, the sensor can also identify diverse targets, primarily leveraging the penetrative and noninvasive nature of light.
A case of familial progressive hyperpigmentation with or without hypopigmentation presenting with hypopigmented striae along the lines of Blaschko
Authors: Hida Tokimasa , Idogawa Masashi , Ishikawa Aki , Okura Masae , Sasaki Satoru , Tokino Takashi , Uhara Hisashi 
Published: September 2024

AbstractFamilial progressive hyperpigmentation with or without hypopigmentation (FPHH) is an autosomal dominant disorder characterized by widespread skin hyperpigmentation, café‐au‐lait spots, and hypopigmented circular macules, resulting from KITLG variants. KITLG, expressed by keratinocytes, binds to KIT on melanocytes, stimulating melanogenesis. Disturbances in the KITLG‐KIT interaction result in diffuse hyperpigmentation in FPHH. However, the mechanisms behind hypopigmented macule formation remain unclear. This report presents a unique FPHH case in a patient with a novel KITLG mutation (Ser78Leu). Notably, the patient showed multiple hypopigmented macules and striae along the lines of Blaschko. Digital polymerase chain reaction analysis of the DNA from skin and blood tissues indicated a copy‐neutral loss of heterozygosity at the KITLG locus, only in the hypopigmented macule. These findings suggest that the hypopigmented macules might result from revertant mosaicism. Conversely, café‐au‐lait spots do not follow the lines of Blaschko and can superimpose on the hypopigmented striae, indicating a distinct pathogenesis. This case contributes to the understanding of the genetic mechanisms in FPHH.
Selling the future state: making property for Sahrawi sovereignty in Western Sahara
Authors: Irwin Randi 
Published: September 2024

AbstractSahrawi refugees and the Sahrawi state‐in‐exile have sought to assert their claims to Western Sahara, Africa's last colony, while exiled in refugee camps in Algeria. Through an examination of the Sahrawi state's use of deferred natural resource contracts, this article explores Sahrawi political action prior to – and in anticipation of – the referendum on self‐determination. I suggest that Sahrawi‐led natural resource contracts operate as a technical financial device that constructs property and enables political action in the anticipation of sovereignty. Through these contracts, the state works to simultaneously produce both itself and its sovereignty. This article explores the new political and economic forms generated by these contracts, which subsequently create a political terrain by which otherwise inaccessible, seemingly off‐limits, resources become productive spaces of opportunity for the development and exercise of sovereignty in the present.
Electrospinning‐based sensing technologies: Opportunities for food applications
Authors: Matinfar Golshan , Ye Haoxin , Bashiry Moein , Hashami Zahra , Yang Tianxi 
Published: September 2024

AbstractThe article explores the science and practice of electrospinning, tracing its history and examining the factors that influence fiber morphology, including the solution composition, processing conditions, and environmental conditions. It offers a thorough examination of electrospinning fundamentals geared toward generating an appropriate platform for creating tailored nanofibers for sensing applications in the food industry. These nanofibers play a critical role in food analysis, sensing, and traceability, and we highlight their effectiveness in identifying pesticide residues, food components, antibiotics, heavy metals, and foodborne pathogens as well as in monitoring the freshness and spoilage of food. The use of electrospinning technology is poised to make sensing platforms more accessible, affordable, and widespread, thereby significantly contributing to the improvement of food quality and safety monitoring practices.
Cannabis‐infused foods: Phytonutrients, health, and safe product innovations
Authors: Fordjour Eric , Manful Charles F. , Khalsamehta Tarsaim S. K. , Armah Abraham , Cheema Mumtaz , Thomas Raymond 
Published: September 2024

AbstractCannabis‐infused foods are currently on the rise in markets all around the world. Meanwhile, there are concerns over the health implications for consumers. Studies have explored the therapeutic potential and nutritional and economic benefits of cannabis usage. Yet, the phytonutrients, processing methods, and health implications of cannabis‐infused foods have not been well explored. This review evaluates existing evidence on the nutritional, processing, safety, and phytonutrient composition of cannabis‐infused food products and their medicinal and functional prospects. Cannabis seeds contain the highest amount of dietary nutrients, while flowers contain the highest amount of bioactive constituents. Oils, butter, seeds, flowers, and leaf extracts are the plant forms currently incorporated into food products such as beverages, baked products, cooking ingredients, functional foods, nutraceuticals, and nootropics. Cannabis‐infused foods have been found to offer therapeutic benefits for pain management, brain function, gut health, and certain cancers. Findings also show significant constraints associated with cannabis‐infused foods regarding dosage guidelines, limited research, efficacy, and long‐term health effects on consumers. This is further worsened by the lack of policies that regulate the industry. To realize the full potential of cannabis use in the food and health industries and in research, regulatory guidelines are needed to control dosages and improve its efficient use in these industries. This will go a long way to ensure the safety of cannabis users and enhance responsible production, marketing, and distribution.
Sulfated Chinese yam polysaccharide exert anti‐inflammatory potential via MAPK/NF‐κB signaling pathways in a co‐culture system and LPS‐induced acute inflammatory mice model
Authors: Chen Xianxiang , Wu Shihua , Tao Xin , He Fengxia , Shen Mingyue 
Published: September 2024

AbstractOur previous study has demonstrated that sulfated Chinese yam polysaccharide (SCYP) can improve immunomodulatory activity in Raw 264.7 cells. However, its anti‐inflammatory is little known. In this study, the anti‐inflammatory effects of SCYP were systematically investigated via the Lipopolysaccharides (LPS)‐induced Raw264.7 cell model, Caco‐2/Raw264.7 co‐culture system, and acute inflammation mice model. The results suggested SCYP promoted the cell proliferation and have no toxicity in Raw264.7 and Caco‐2 cells at the concentration of 200 µg/mL. Moreover, when treated with SCYP, the production of pro‐inflammatory cytokines (interleukin [IL]‐1β, IL‐6, and tumor necrosis factor‐α) reduced significantly in Raw264.7 via the MAPK/NF‐κB pathway. In the Caco‐2/Raw264.7 co‐cultured system, SCYP could regulate inflammation reaction by improving intestinal barrier, which might prevent systemic inflammation. Further, systemic inflammation was alleviated by SCYP in LPS‐induced acute inflammation mice through MAPK/NF‐κB pathway.Practical ApplicationThese results supported that SCYP may be used as an anti‐inflammation agent in the functional food field.
Perceptions and expectations of an artificially intelligent physical activity digital assistant — A focus group study
Authors: Vandelanotte Corneel , Hodgetts Danya , Peris D.L.I.H.K. , Karki Ashmita , Maher Carol , Imam Tasadduq , Rashid Mamunur , To Quyen , Trost Stewart 
Published: September 2024

AbstractArtificially intelligent physical activity digital assistants that use the full spectrum of machine learning capabilities have not yet been developed and examined. This study aimed to explore potential users' perceptions and expectations of using such a digital assistant. Six 90‐min online focus group meetings (n = 45 adults) were conducted. Meetings were recorded, transcribed and thematically analysed. Participants embraced the idea of a ‘digital assistant’ providing physical activity support. Participants indicated they would like to receive notifications from the digital assistant, but did not agree on the number, timing, tone and content of notifications. Likewise, they indicated that the digital assistant's personality and appearance should be customisable. Participants understood the need to provide information to the digital assistant to allow for personalisation, but varied greatly in the extent of information that they were willing to provide. Privacy issues aside, participants embraced the idea of using artificial intelligence or machine learning in return for a more functional and personal digital assistant. In sum, participants were ready for an artificially intelligent physical activity digital assistant but emphasised a need to personalise or customise nearly every feature of the application. This poses challenges in terms of cost and complexity of developing the application.
Transcranial direct current stimulation enhances the protective effect of isoflurane preconditioning on cerebral ischemia/reperfusion injury: A new mechanism associated with the nuclear protein Akirin2
Authors: Kong Xiangyi , Lyu Wenyuan , Lin Xiaojie , Feng Hao , Xu Lin , Li Chengwei , Sun Xinyi , Lin Chunlong , Li Jianjun , Wei Penghui 
Published: September 2024

AbstractAimsIschemic stroke is a major cause of disability and mortality worldwide. Transcranial direct current stimulation (tDCS) and isoflurane (ISO) preconditioning exhibit neuroprotective properties. However, it remains unclear whether tDCS enhances the protective effect of ISO preconditioning on ischemic stroke, and the underlying mechanisms are yet to be clarified.MethodA model of middle cerebral artery occlusion (MCAO), a rat ischemia–reperfusion (I/R) injury model, and an in vitro oxygen–glucose deprivation/re‐oxygenation (O/R) model of ischemic injury were developed. ISO preconditioning and tDCS were administered daily for 7 days before MCAO modeling. Triphenyltetrazolium chloride staining, modified neurological severity score, and hanging‐wire test were conducted to assess infarct volume and neurological outcomes. Untargeted metabolomic experiments, adeno‐associated virus, lentiviral vectors, and small interfering RNA techniques were used to explore the underlying mechanisms.ResultstDCS/DCS enhanced the protective effects of ISO pretreatment on I/R injury‐induced brain damage. This was evidenced by reduced infarct volume and improved neurological outcomes in rats with MCAO, as well as decreased cortical neuronal death after O/R injury. Untargeted metabolomic experiments identified oxidative phosphorylation (OXPHOS) as a critical pathological process for ISO‐mediated neuroprotection from I/R injury. The combination of tDCS/DCS with ISO preconditioning significantly inhibited I/R injury‐induced OXPHOS. Mechanistically, Akirin2, a small nuclear protein that regulates cell proliferation and differentiation, was found to decrease in the cortex of rats with MCAO and in cortical primary neurons subjected to O/R injury. Akirin2 functions upstream of phosphatase and tensin homolog deleted on chromosome 10 (PTEN). tDCS/DCS was able to further upregulate Akirin2 levels and activate the Akirin2/PTEN signaling pathway in vivo and in vitro, compared with ISO pretreatment alone, thereby contributing to the improvement of cerebral I/R injury.ConclusiontDCS treatment enhances the neuroprotective effects of ISO preconditioning on ischemic stroke by inhibiting oxidative stress and activating Akirin2‐PTEN signaling pathway, highlighting potential of combination therapy in ischemic stroke.
The potential of roadside verges as insect habitat: Road salt has few effects on monarch butterfly performance and migration
Authors: Hund Amanda K. , Mitchell Timothy S. , Ramίrez M. Isabel , Zambre Amod , Hagg Lili , Stene Anne , Porter Karilyn , Carper Adrian , Agnew Lauren , Shephard Alexander M. , Kobiela Megan E. , Oberhauser Karen S. , Taylor Orley R. , Snell‐Rood Emilie C. 
Published: September 2024

AbstractRoadside habitat has been touted as a conservation opportunity for insect pollinators, including the declining monarch butterfly. The spectacular monarch migration is under threat from the loss of habitat and the decline of their milkweed host plants. In the northern part of their range, roadsides could potentially produce millions of monarchs annually due to high densities of milkweed; however, roadside milkweed can accumulate chemicals from roads, such as sodium from road salt. Controlled lab studies have shown mixed effects of sodium on monarch development: small increases can be beneficial as sodium is an important micronutrient in brain and muscle development, but large increases can sometimes decrease survival. It is unclear how dietary sodium affects performance in ecologically relevant conditions and the migration itself. In this experiment, we raised monarchs outdoors, in migration‐inducing conditions, on milkweed sprayed with three levels of sodium chloride. We released 2464 tagged monarchs and held an additional 246 for further lab assays. While our recovery rates to the wintering grounds were low (N = 7 individuals), individuals from all three sodium chloride treatments made it to Mexico. Butterflies reared on control milkweed and low salt concentrated sodium in their tissues, while those on high salt diets excreted sodium, suggesting high salt levels were above a physiological optimum. There were no effects of treatment on wing coloration, survival, body size, immunity, or parasite prevalence. Taken together, our results suggest that monarchs are robust to levels of sodium in milkweeds found along roadsides, which is promising with respect to the toxicity of roadside plants.
High plasticity in diapause responses benefits bark beetles in a changing climate
Authors: Hofmann Sven , Kautz Markus , Schebeck Martin 
Published: September 2024

Abstract
Numerous insects evolved diapause to cope with seasonally re‐occurring adverse conditions, affecting multiple life‐history traits, including reproduction, survival and voltinism. The spruce bark beetle Ips typographus—a major disturbance agent in spruce‐dominated forests of Eurasia—enters reproductive diapause to survive harsh winters. Induction of facultative diapause is mainly regulated by short daylengths, but modified by warm temperatures, potentially postponing entry into diapause. Knowledge on the interplay of these cues under natural conditions is still lacking despite its importance for phenology and management in a changing climate.
We conducted a comprehensive field study over 3 years along an elevational gradient in South‐West Germany to quantify diapause expression in I. typographus under a broad range of natural photoperiod and temperature conditions.
Most individuals entered diapause in mid‐August (daylengths <14 h), regardless of temperature. However, some individuals still reproduced at warm temperatures (>22–26°C) in late October (daylengths ~10 h), even following cold nights below 5°C. However, fecundity of these late‐breeding individuals was reduced.
In comparison to previous findings, we revealed a later diapause induction in I. typographus due to warm temperatures. Within‐population variability in the timing of diapause induction is an effective strategy to increase the amount of late‐breeding individuals and thus voltinism in a warming climate. Bark beetle phenology models should hence (i) include temperature as diapause‐modifying cue in addition to photoperiod and (ii) consider potential differences between populations from diverse geographic origins.
The impact of social media influencers on the financial market performance of firms
Authors: Keasey Kevin , Lambrinoudakis Costas , Mascia Danilo V. , Zhang Zhengfa 
Published: September 2024

AbstractDespite the huge growth in the number of influencers and their use by firms, there is a lack of analysis of how social media influencers affect the financial market performance of firms. Anecdotal evidence suggests mega influencers can impact the stock prices of firms via social media. We ask whether such an effect is generalizable to all mega influencers and other financial market characteristics of firms. Using a hand‐collected data set of 16,156,419 mega influencer posts on Instagram, we find that mega influencers affect investors' attention, volatility and trading volume but not stock returns. It takes top influencers with extreme sentiment posts to affect returns and, even here, the effect is short‐lived.
Precipitation and temperature timings underlying bioclimatic variables rearrange under climate change globally
Authors: Bede‐Fazekas Ákos , Somodi Imelda 
Published: September 2024

AbstractModeling how climate change may affect the potential distribution of species and communities typically utilizes bioclimatic variables. Distribution predictions rely on the values of the bioclimatic variable (e.g., precipitation of the wettest quarter). However, the ecological meaning of most of these variables depends strongly on the within‐year position of a specific climate period (SCP), for example, the wettest quarter of the year, which is often overlooked. Our aim was to determine how the within‐year position of the SCPs would shift (SCP shift) in reaction to climate change in a global context. We calculated the deviations of the future within‐year position of the SCPs relative to the reference period. We used four future time periods, four scenarios, and four CMIP6 global climate models (GCMs) to provide an ensemble of expectations regarding SCP shifts and locate the spatial hotspots of the shifts. Also, the size and frequency of the SCP shifts were subjected to linear models to evaluate the importance of the impact modeler's decision on time period, scenario, and GCM. We found ample examples of SCP shifts exceeding 2 months, with 6‐month shifts being predicted as well. Many areas in the tropics are expected to experience both temperature and precipitation‐related shifts, but precipitation‐related shifts are abundantly predicted for the temperate and arctic zones as well. The combined shifts at the Equator reinforce the likelihood of the emergence of no‐analogue climates there. The shifts become more pronounced as time and scenario progress, while GCMs could not be ranked in a clear order in this respect. For most SCPs, the modeler's decision on the GCM was the least important, while the choice of time period was typically more important than the choice of scenario. Future predictive distribution models should account for SCP shifts and incorporate the phenomenon in the modeling efforts.
I'm brown and I'm bright: Using collective storying to disrupt the white‐centering of successful girlhood
Authors: Gaerlan Eunice , Cameron Yael 
Published: September 2024

AbstractWhat might it mean to reimagine brown‐girl‐as‐failure to brown‐girl‐as‐success? This article draws on findings from an empirical research study of academically successful teenage girls from Aotearoa New Zealand. In this paper we focus on what it means to be an intelligent and successful young brown woman in the context of the contemporary white‐centering of meritocratic success, and the oppressive narrative that brown girls are not bright. Using a creative methodology, Laurel Richardson's collective storying and Patricia Leavy's fiction‐based research, the paper engages in forms of creative analytic practice and new knowledge representation, which prioritize authentic voice and understanding of the young women participants' lived experiences. Collective stories were used in the study to challenge existing public discourses of girls and success, including the white‐centering of such depictions, and to create narratives that participants could identify with, particularly those that were often unspoken but widely experienced. Using collective stories in the study offered a space of resonance with participants who could engage with the stories during the research process and contribute to their (re)storying. The interplay between the theoretics of methodological creativity and the symbolic violence of a colonial positioning of successful girlhood offers a novel contribution to girlhood studies. Through collective storying and a further interweaving of poetic voice, the disruption of the narrative of deficit offers remembering and revalidation of brown success.