bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human ovarian extracellular vesicles proteome from polycystic ovary syndrome patients associate with follicular development alterations
Authors: Couty Noemie , Estienne Anthony , Le Lay Soazig , Rame Christelle , Chevaleyre Claire , Allard‐Vannier Emilie , Péchoux Christine , Guerif Fabrice , Vasseur Claudine , Aboulouard Soulaimane , Salzet Michel , Dupont Joelle , Froment Pascal 
Published: October 2024

AbstractThe development of the ovarian follicle requires the presence of several factors that come from the blood and follicular cells. Among these factors, extracellular vesicles (EVs) represent an original communication pathway inside the ovarian follicle. Recently, EVs have been shown to play potential roles in follicular development and reproduction‐related disorders, including the polycystic ovary syndrome (PCOS). The proteomic analysis of sEVs isolated from FF in comparison to sEVs purified from plasma has shown a specific pattern of proteins secreted by ovarian steroidogenic cells such as granulosa cells. Thus, a human granulosa cell line exposed to sEVs from FF of normal patients increased their progesterone, estradiol, and testosterone secretion. However, if the sEVs were derived from FF of PCOS patients, the activity of stimulating progesterone production was lost. Stimulation of steroidogenesis by sEVs was associated with an increase in the expression of the StAR gene. In addition, sEVs from FF increased cell proliferation and migration of granulosa cells, and this phenomenon was amplified if sEVs were derived from PCOS patients. Interestingly, STAT3 is a protein overexpressed in sEVs from PCOS patients interacting with most of the cluster of proteins involved in the phenotype observed (cell proliferation, migration, and steroid production) in granulosa cells. In conclusion, this study has demonstrated that sEVs derived from FF could regulate directly the granulosa cell activity. The protein content in sEVs from FF is different in the case of PCOS syndrome and could perturb the granulosa cell functions, including inflammation, steroidogenesis, and cytoskeleton architecture.
Magnetic Resonance Imaging Can Predict Hamstring or Quadriceps Tendon Autograft Diameter in Pediatric or Adolescent Anterior Cruciate Ligament Reconstruction: A Systematic Review
Authors: Vivekanantha Prushoth , Sun Bryan , Parasu Naveen , de SA Darren 
Published: October 2024

Objective:
Insufficient graft diameter is an important factor that contributes to failure rates after anterior cruciate ligament reconstruction (ACLR). Although modalities, such as magnetic resonance imaging (MRI), have been well investigated in the adult population to predict graft diameter preoperatively, it is unclear whether similar strategies can be used in the pediatric population. This review aims to evaluate the utility of MRI in the preoperative estimation of autograft parameters in pediatric or adolescent patients undergoing ACLR.

Methods:
Three databases were searched on January 31, 2024. The authors adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Revised Assessment of Multiple Systematic Reviews guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. Data on demographics, graft characteristics, MRI imaging techniques, and statistical analyses correlating MRI parameters with graft length or diameter were recorded.

Results:
Five studies consisting of 450 patients were included in this review (48.4% females). The mean (SD) age at ACLR was 14.7 (6.8) years. Three studies using hamstring tendon (HT) autografts found that combined semitendinosus and gracilis tendon cross-sectional area (ST+GT CSA) were able to predict graft diameter. One study reported an ST+GT CSA cutoff of 31.2 mm2 to have an 80% and 74% sensitivity and specificity, respectively, in predicting HT autograft diameter above 8 mm. Two studies using quadriceps tendon (QT) autografts found that tendon thickness on sagittal MRI view was able to predict graft diameter. One study reported a QT cutoff of 6.7 mm to have 97.5% and 46.6% sensitivity and specificity, respectively, in predicting the diameter of the QT graft to be above 8 mm. Associations between MRI parameters and graft length were not reported.

Conclusion:
Although there is limited evidence, ST+GT CSA and QT thickness on sagittal view on MRI can be used to predict intraoperative HT and QT autograft diameter, respectively, in pediatric or adolescent ACLR. Future investigations should investigate correlations between imaging parameters and graft length, especially when using QT autografts in the pediatric population.

Level of Evidence:
Level III.
Evaluation of a Physiologic-Driven Closed-Loop Resuscitation Algorithm in an Animal Model of Hemorrhagic Shock
Authors: Pinsky Michael R. , Gomez Hernando , Wertz Anthony , Leonard Jim , Dubrawski Artur , Poropatich Ronald 
Published: October 2024

OBJECTIVES:
Appropriate resuscitation from hemorrhagic shock is critical to restore tissue perfusion and to avoid over-resuscitation. The objective of this study was to test the ability of a closed-loop diagnosis and resuscitation algorithm called resuscitation from shock using functional hemodynamic monitoring using invasive monitoring (ReFit1) and minimally invasive monitoring (ReFit2) to identify, treat, and stabilize a porcine model of severe hemorrhagic shock.

DESIGN:
We created a ReFit algorithm using dynamic hemodynamic parameters of pulse pressure variation (PPV), stroke volume variation (SVV), dynamic arterial elastance (Eadyn = PPV/SVV), driven by mean arterial pressure (MAP), mixed venous oxygen saturation, and heart rate targets to define the need for fluids, vasopressors, and inotropes.

SETTING:
University-based animal laboratory.

SUBJECTS:
Twenty-seven female pigs.

INTERVENTIONS:
Anesthetized, intubated, and ventilated (8 mL/kg) pigs were bled at 10 mL/min until a MAP of less than 40 mm Hg, held for 30 minutes, then resuscitated. The ReFit algorithm used the above dynamic parameters to drive computer-controlled infusion pumps to deliver blood, lactated Ringer’s solution, norepinephrine, and in ReFit1 dobutamine. In four animals, after initial resuscitation from hemorrhagic shock, the ability of the ReFit1 algorithm to treat acute air embolism-induced pulmonary hypertension and right heart failure was also tested.

MAIN RESULTS:
In 10 ReFit1 and 17 ReFit2 animals, the time to stabilization from shock was not dissimilar to open controlled resuscitation performed by an expert physician (52 ± 12, 50 ± 13, and 60 ± 15 min, respectively) with similar amounts of fluids and norepinephrine needed. In four ReFit1 animals after initial stabilization, the algorithm successfully resuscitated the animals after inducing an acute air embolism right heart failure, with all animals recovering stability within 30 minutes.

CONCLUSIONS:
Our physiologically based functional hemodynamic monitoring-centered closed-loop resuscitation system can effectively diagnose and treat cardiovascular shock due to hemorrhage and air embolism.
Menopausal hormone therapy for breast cancer survivors
Authors: Huguenin Annabelle 
Published: October 2024

Purpose of review
The global incidence of breast cancer continues to increase and increasing efficacy of treatments has improved overall prognosis and survival with a resulting requisite focus on improving quality of life after cancer. Treatment inevitably results in symptoms of menopause and these symptoms may be more severe after cancer treatment compared to natural menopause and may pose a potential risk of early treatment discontinuation. Consequently, the global burden of successfully managing these symptoms is significant.

Recent findings
There is a discordance between randomized and observational data regarding the risk of systemic menopausal hormone therapy and breast cancer recurrence; hormone receptor status is significant to recurrence risk in this setting. The systemic absorption of local oestrogen is not a consistent surrogate marker for cancer recurrence and clinical data has not demonstrated a consistent increase in the risk of cancer recurrence with local therapy.

Summary
Nonhormonal treatments remain first-line management of menopause symptoms in breast cancer survivors to minimize the risk of cancer recurrence. However, severe symptoms not responding to nonhormonal interventions require a multidisciplinary, patient-centred approach to discuss the evidence base for hormonal treatments.
Genetic Variants Associated With a History of Glaucoma Surgery in Japanese Patients With Primary Open Angle Glaucoma
Authors: Mabuchi Fumihiko , Tanaka-Mabuchi Nakako , Sakurada Yoichi , Yoneyama Seigo , Yamagata Zentaro , Kashiwagi Kenji ,   for the Japan Glaucoma Society Omics Group (JGS-OG)
Published: October 2024

Précis:
The genetic risk score (GRS) of genetic variants associated with intraocular pressure (IOP) elevation, but not those associated with optic nerve vulnerability, was associated with a history of glaucoma surgery in Japanese patients with primary open angle glaucoma (POAG).

Objective:
To investigate genetic variants associated with a history of glaucoma surgery in Japanese patients with POAG.

Methods:
Japanese patients with POAG (n = 468), including normal tension glaucoma (n = 246) and high tension glaucoma (n = 222), and control subjects (n = 246) were genotyped for 22 genetic variants predisposing to POAG, which can be classified into those associated with IOP elevation (IOP-related genetic variants) and optic nerve vulnerability independent of IOP (optic nerve-related genetic variants). The unweighted and weighted GRSs of 17 IOP-related, 5 optic nerve-related, and all 22 genetic variants were calculated, and the association between the GRS and a history of glaucoma surgery was evaluated.

Results:
There was a significant association (odds ratio 1.13 per unweighted GRS, 95% CI: 1.03 to 1.24, P = 0.0093) between IOP-related unweighted GRS and a history of glaucoma surgery. A significant association (odds ratio 1.09 per 0.1 weighted GRS, 95% CI: 1.04 to 1.14, P = 0.00022) was also found between IOP-related weighted GRS and a history of glaucoma surgery. The IOP-related GRS was positively correlated with the need for glaucoma surgery. The mean of IOP-related unweighted and weighted GRS in patients with POAG with a history of glaucoma surgery were significantly higher (P = 0.013 and P = 0.00031, respectively) than those in patients with POAG without a history of glaucoma surgery.

Conclusions:
IOP-related, but not optic nerve-related, genetic variants were associated with a history of glaucoma surgery in Japanese patients with POAG. These results indicate that IOP elevation induced by IOP-related genetic variants rather than optic nerve vulnerability induced by optic nerve-related genetic variants may play an important role in requiring glaucoma surgery.
Analysis of Factors and Clinical Outcomes of Planned Tracheal Extubation Failure in Neurosurgical Intensive Care Unit Patients
Authors: Wu Haotian , Shen Jinsong , Xu Yan 
Published: October 2024

ABSTRACT

BACKGROUND: Planned tracheal extubation failure is a common occurrence among patients in the neurosurgical intensive care unit (NICU) because of the complex nature of neurocritical injuries, and the failure could result in a poor prognosis. METHODS: We observed and recorded the patients with tracheal intubation in the NICU of a hospital in Shanghai from June 2021 to December 2022 and analyzed data from planned tracheal extubation, categorizing patients by success or failure, and compared outcomes between the two groups while investigating contributing factors. RESULTS: A total of 156 patients were included, 133 (85.3%) of whom were successfully extubated and 23 (14.7%) were not. The results of logistic regression analysis demonstrated that the Glasgow Coma Scale score before extubation (OR, 0.643; 95% CI, 0.444–0.931; P = .020) and the frequency of respiratory secretions suctioning before tracheal extubation (OR, 0.098; 95% CI, 0.027–0.354; P < .001) were independent risk factors for extubation failure. We also found that the extubation failure group experienced a significantly longer ICU stay and incurred higher hospitalization costs. CONCLUSIONS: Poor Glasgow Coma Scale scores and a high frequency of respiratory secretions suctioning before tracheal extubation were the main factors contributing to tracheal extubation failure in NICU patients. To avoid tracheal extubation failure and adverse outcomes, these two factors should be carefully assessed before tracheal extubation.
Indocyanine green fluorescence angiography in the management of intestinal injuries following penetrating abdominal trauma: a case-control study comparing postoperative outcomes
Authors: Patel Mohamed Q. , Osterkamp Jens TF , Buitendag Johan JP , Forgan Timothy R. , Steyn Elmin 
Published: October 2024

Background:
The surgical management of penetrating hollow visceral injuries includes primary repair or exteriorization. Tissue perfusion at the site of gastrointestinal suture repair may be challenging to assess and is vulnerable to local energy transfer-related injury, micro- or macro-circulatory insufficiency, or splanchnic vasoconstriction for various reasons. Breakdown of suture lines can lead to potentially life-threatening complications. The intraoperative use of indocyanine green fluorescence angiography (ICG-FA) may reduce the risk of postoperative morbidity and mortality by ensuring optimal tissue perfusion at the chosen site of suture repair.

Materials and methods:
The authors conducted a retrospective review of the postoperative complications, length of ICU stay, and length of hospital stay in patients undergoing laparotomy, with and without ICG-FA for penetrating abdominal trauma at a Level One Trauma Center in Cape Town, South Africa.

Results:
One hundred patients were included in the study, of which 20 underwent laparotomy with ICG-FA, and 80 did not. The overall complication rate was significantly lower in the ICG-FA group (OR 0.336, p=0.0412). The anastomotic leak rates in the ICG-FA and control groups were 0% and 6.25%, respectively (p=0.5799). Revision surgery was required in 2 and 14 patients in the ICG-FA and control groups, respectively (OR 0.524, p=0.516). The mean length of stay in the hospital showed no statistical difference, 8.6 and 5.3 days for the ICG-FA and control groups, respectively (p=0.092). The mean length of ICU stay was 6.3 and 2.3 days for the ICG-FA and control groups, respectively (p=0.1642).

Conclusion:
Lower levels of overall postoperative complications and lower rates of revision surgery in patients undergoing laparotomy with ICG-FA are promising. Non-significant findings regarding the relationship between the usage of ICG-FA and anastomotic leak rates suggest the need for larger randomized studies.
METTL16 inhibits pancreatic cancer proliferation and metastasis by promoting MROH8 RNA stability and inhibiting CAPN2 expression – experimental studies
Authors: Yi Tingzhuang , Wang Chunming , Ye Xia , Lin Jie , Lin Cheng , Qin Fengzhen , Yang Wanlin , Ye Yulu , Ning Dengchong , Lan Jinyan , Li Huafu , Luo Chunying , Ma Jian , Wei Zhongheng 
Published: October 2024

Background:
N6-methyladenosine (m6A) modification plays a crucial role in the progression of various cancers, including pancreatic cancer, by regulating gene expression. However, the specific mechanisms by which m6A affects pancreatic cancer metastasis remain unclear. This study aims to elucidate the role of METTL16, an m6A writer gene, in regulating core genes such as CAPN2 and MROH8, influencing tumor growth and metastasis.

Materials and Methods:
Transcriptomic data from pancreatic cancer patients in The Cancer Genome Atlas (TCGA) were analyzed to identify m6A-related genes. We performed correlation and survival analyses to uncover core genes influenced by m6A expression. Functional assays, including METTL16 knockdown and overexpression experiments, were conducted in pancreatic cancer cell lines, patient-derived organoids, and animal models. Immunofluorescence, co-immunoprecipitation (Co-IP), and m6A-specific quantitative PCR were used to validate protein interactions and m6A modifications. Chromatin immunoprecipitation (ChIP) analysis was utilized to investigate transcription factor binding at gene promoter regions.

Results:
METTL16 and METTL3 were identified as key m6A regulators associated with improved prognosis in pancreatic cancer patients (P<0.05). CAPN2, CHMP2B, ITGA3, ITGA6, ITPR1, and RAC1 were identified as core genes linked to m6A expression, all significantly correlated with patient prognosis (P<0.05). METTL16 overexpression significantly inhibited tumor growth and metastasis (P<0.001) by downregulating CAPN2 through an indirect mechanism involving the transcription factor TBP and the gene MROH8. MROH8 negatively regulated CAPN2 by promoting TBP degradation, with METTL16 enhancing MROH8 mRNA stability through m6A modifications (P<0.01). Functional assays demonstrated that METTL16 and YTHDC2 (an m6A reader) collaboratively enhanced MROH8 mRNA stability, thereby inhibiting CAPN2 expression and reducing tumor proliferation and metastasis (P<0.001).

Conclusion:
This study reveals a novel regulatory axis involving METTL16, MROH8, and TBP that modulates CAPN2 expression, contributing to the suppression of pancreatic cancer progression. The METTL16–MROH8–TBP–CAPN2 pathway offers potential therapeutic targets for pancreatic cancer treatment, highlighting the significance of m6A modifications in tumor regulation. Further clinical validation is needed to confirm these findings in human patients.
Guiding treatment decisions in renal cell carcinoma: the role of biomarkers and clinical factors
Authors: Huebner-Resch Irene , Schmidinger Manuela 
Published: October 2024

Purpose of review
Immune checkpoint inhibitors (ICIs) have transformed the treatment landscape for metastatic renal cell carcinoma (mRCC), significantly improving overall survival and achieving durable responses. This review is timely due to the increasing number of ICI-based regimens now considered standard care for RCC. There is an urgent need to identify reliable biomarkers that can predict therapeutic responses and resistance, a key challenge in current research.

Recent findings
While tumor-specific factors such as pathological characteristics, genomic mutations, and transcriptional profiles have been extensively studied, no definitive predictive biomarker has yet emerged. Additionally, advanced technologies are being explored to address tumor heterogeneity. Recent research has focused on novel areas such as the microbiome, radiomics, and spatial transcriptomics, which show promise as potential biomarkers.

Summary
The translation of these emerging biomarker findings into clinical practice is essential to improving personalized treatment strategies for RCC. Until reliable biomarkers are clinically available, clinical factors may play a pivotal role in guiding individualized treatment decisions to optimize patient outcomes.
Necrotizing enterocolitis in term infants with congenital heart disease and preterm infants after surgery
Authors: Hadian Hooman , Esparham Ali , Sheikhbahaei Erfan , Zefreh Hamidreza , Hosseinpour Mehrdad 
Published: October 2024

Background:
The purpose of this study was to report and compare the surgical outcomes of preterm-related necrotizing enterocolitis (PNEC) and congenital heart disease (CHD)-related necrotizing enterocolitis (CDNEC).

Materials and Methods:
Preterm infants without CHD and term infants with CHD who developed NEC and needed surgery were included in this cohort study between 2018 and 2021 at our university-affiliated hospital. A board-certified pediatric cardiologist examined all the patients with echocardiographic and cardiac examinations to diagnose CHD, and a pediatric surgeon evaluated the neonates for surgical indications. Patient characteristics, pregnancy history, and surgical and mortality outcomes were collected from the infants’ medical records.

Results:
Forty infants with NEC were included. The CDNEC and PNEC groups consisted of 18 and 22 infants, respectively. Although the disease-specific mortality rate was higher in the CDNEC group than in the PNEC group, the difference was insignificant (55.5% and 40.9%, respectively, P=0.36). There was no statistically significant difference in the time of NEC occurrence, location, type of surgery, or indications for surgery between the two groups.

Conclusion:
Despite the pathophysiologies of CDNEC and PNEC being different, the characteristics, pregnancy history, surgical, and mortality outcomes of these two types are similar in infants who undergo surgical treatment.
Epispadic accessory urethra in an 8-month-old female with urethral duplication: a case report
Authors: Askar Ayed S. , Altarraf Tarraf A. , Mohamed Shrouk F. 
Published: October 2024

Introduction and importance:
Urethral duplication is an uncommon urogenital congenital disability that presents as an accessory urethra in an atypical location. This condition is often coupled with other congenital anomalies like bladder exstrophy, pseudohermaphroditism, and renal disorders.

Case presentation:
The authors report a case of a female infant aged 8 months with urethral duplication, showing an unusual urinary opening in the prepubic area and urinary discharge. A comprehensive diagnostic assessment, consisting of a clinical exam, imaging tests, and cystoscopy, verified the existence of an accessory urethra and the normal one in epispadias. The patient had a successful surgery to remove the accessory tract with primary closure.

Clinical discussion:
Urethral duplication is associated with diverse clinical signs and symptoms. Accurate diagnosis needs thorough imaging studies, and treatment should be tailored according to the type and anatomical location of the accessory tract.

Conclusion:
The histological confirmation of urethral duplication was obtained following the surgical excision of the additional tract. This case underscores the significance of accurate diagnosis and treatment approaches to manage this case.
Describing Clinical Nurse Specialist Practice: A Mixed-Methods Study
Authors: Kilpatrick Kelley , Tewah Ruth , Tchouaket Eric , Jokiniemi Krista , Bouabdillah Naima , Biron Alain , Emed Jessica , Martel Brigitte , Atallah Renée , Jabbour Mira , Bryant-Lukosius Denise 
Published: October 2024

Purpose/Aims
To describe clinical nurse specialist practice in Québec, Canada, and propose a dashboard to track role dimensions and outcomes.

Design
Sequential mixed-methods study across 6 sites in Québec (June 2021 to May 2022).

Methods
Phase 1: Focus groups (n = 8) and individual interviews (n = 3) were conducted to adapt a time and motion tool. Phase 2: Time and motion studies (n = 25; 203 hours 5 minutes) described clinical nurse specialist practice. Phase 3: A rapid literature review and study participants’ feedback informed the dashboard’s development. Analysis: Descriptive statistics, with content analysis for qualitative data.

Results
The proportion of time clinical nurse specialists spent in role dimensions included clinical (22.8%), education (11.2%), administrative/leadership (48.6%), research (9.6%), and personal (7.7%). On average, they spent 17% of work time with patients, but this varied across specialties and locations. Key dashboard characteristics and uses were identified.

Conclusions
Important differences were noted in clinical nurse specialist time spent in activities across specialties and regions in Québec. Approximately one-fifth of work time was spent in direct patient care. Additional research is needed to examine the link between clinical nurse specialist practice and outcomes in other jurisdictions and test the implementation of a dashboard to make their practice more visible.