bluuebunny/crossref_metadata_2025_split huggingface dataset:
Language and Identity Construction: The Case of a Refugee Digital Bricoleur
Authors: Karam Fares J. 
Published: December 2017

AbstractThe United States is the biggest resettlement country of refugees referred by the United Nations High Commissioner for Refugees; however, educational resettlement efforts have been unsuccessful in responding to the needs of refugee students, and educational research has thus far presented a deficit‐oriented narrative that ignores the skills and agency of these students. This study challenges such deficit‐oriented presentations by examining how Zein, an adolescent refugee English learner, uses language to construct his identity and position himself as a digital bricoleur (someone creatively using different materials to produce new artifacts). We learn from Zein that classroom tasks with multimodal dimensions can provide spaces for refugee students to negotiate their engagement with classroom tasks to better align them to their interests and identities, make connections between their school‐based and out‐of‐school literacy practices, and practice their agency to produce counternarratives that challenge deficit perspectives of refugee students.
Bacterial nanocellulose‐IKVAV hydrogel matrix modulates melanoma tumor cell adhesion and proliferation and induces vasculogenic mimicry in vitro
Authors: Reis Emily M. dos , Berti Fernanda V. , Colla Guilherme , Porto Luismar M. 
Published: December 2017

AbstractVasculogenic mimicry process has generated great interest over the past decade. So far, however, there have been only a few matrices available that allow us to study that process in vitro. Here, we have developed an innovative hydrogel platform with defined composition that mimics the structural architecture and biological functions of the extracellular matrix for vasculogenic mimicry of human melanoma cells (SK‐MEL‐28). We chemically immobilized IKVAV peptide on bacterial nanocellulose (BNC) fibers. BNC‐IKVAV hydrogel was found to improve the adhesion and proliferation of SK‐MEL‐28 cells on the top and bottom surfaces. Particularly, the bottom surface of BNC‐IKVAV induced SK‐MEL‐28 cells to organize themselves as well‐established networks related to the vasculogenic mimicry process. Finally, our results showed that not only BNC‐IKVAV but also BNC hydrogels can potentially be used as a three‐dimensional platform that allows the screening of antitumor drugs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2741–2749, 2018.
Cardiac tissue regeneration: A preliminary study on carbon‐based nanotubes gelatin scaffold
Authors: Cabiati Manuela , Vozzi Federico , Gemma Federica , Montemurro Francesca , De Maria Carmelo , Vozzi Giovanni , Domenici Claudio , Del Ry Silvia 
Published: December 2017

AbstractThe aim of this study was set‐up and test of gelatin and carbon nanotubes scaffolds. Gelatin‐based (5%) genipin cross‐linked (0.2%) scaffolds embedding single‐walled carbon nanotubes (SWCNTs, 0.3, 0.5, 0.7, 0.9, and 1.3% w/w) were prepared and mechanically/electrically characterized. For biological evaluation, H9c2 cell line was cultured for 10 days. Cytotoxicity, cell growth and differentiation, immunohistochemistry, and real‐time PCR analysis were performed. Myoblast and cardiac differentiation were obtained by serum reduction to 1% (C1%) and stimulation with 50 nM all trans‐retinoic acid (CRA), respectively. Immunohistochemistry showed elongated myotubes in C1% while round and multinucleated cells in CRA with also a significantly increased expression of natriuretic peptides (NP) and ET‐1 receptors in parallel with a decreased ET‐1. On scaffolds, cell viability was similar for Gel–SWCNT0.3%/0.9%; NP and ET systems expression decreased in both concentrations with respect to control and CX‐43, mainly due to a lacking of complete differentiation in cardiac phenotype during that time. Although further analyses on novel biomaterials are necessary, these results represent a useful starting point to develop new biomaterial‐based scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2750–2762, 2018.
Osteoprotection Through the Deletion of the Transcription Factor Rorβ in Mice
Authors: Farr Joshua N , Weivoda Megan M , Nicks Kristy M , Fraser Daniel G , Negley Brittany A , Onken Jennifer L , Thicke Brianne S , Ruan Ming , Liu Hong , Forrest Douglas , Hawse John R , Khosla Sundeep , Monroe David G 
Published: December 2017

ABSTRACT

There is a clinical need to identify new molecular targets for the treatment of osteoporosis, particularly those that simultaneously inhibit bone resorption while stimulating bone formation. We have previously shown in overexpression studies that retinoic acid receptor-related orphan receptor β (Rorβ) suppresses in vitro osteoblast differentiation. In addition, the expression of Rorβ is markedly increased in bone marrow–derived mesenchymal stromal cells with aging in both mice and humans. Here we establish a critical role for Rorβ in regulating bone metabolism using a combination of in vitro and in vivo studies. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 gene editing to demonstrate that loss of Rorβ in osteoblasts enhances Wnt signaling, specifically through increased recruitment of β-catenin to T-cell factor/lymphoid enhancer factor (Tcf/Lef) DNA binding sites in the promoters of the Wnt target genes Tcf7 and Opg. This resulted in increased osteogenic gene expression and suppressed osteoclast formation through increased osteoprotegerin (OPG) secretion in Rorβ-deficient cells. Consistent with our in vitro data, genetic deletion of Rorβ in both female and male mice resulted in preserved bone mass and microarchitecture with advancing age due to increased bone formation with a concomitant decrease in resorption. The improved skeletal phenotype in the Rorβ–/– mice was also associated with increased bone protein levels of TCF7 and OPG. These data demonstrate that loss of Rorβ has beneficial skeletal effects by increasing bone formation and decreasing bone resorption, at least in part through β-catenin–dependent activation of the Wnt pathway. Thus, inhibition of Rorβ represents a novel approach to potentially prevent or reverse osteoporosis. © 2017 American Society for Bone and Mineral Research.
Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation
Authors: Wang Yongkui , Xiao Min , Tao Chen , Chen Jing , Wang Zhenyu , Yang Jun , Chen Zhenguo , Zou Zhipeng , Liu Anling , Cai Daozhang , Jiang Yu , Ding Changhai , Li Mangmang , Bai Xiaochun 
Published: December 2017

ABSTRACT

Osteoblasts provide a microenvironmental niche for B-cell commitment and maturation in the bone marrow (BM). Any abnormity of osteoblasts function may result in the defect of B lymphopoiesis. Signaling from mechanistic target of rapamycin complex 1 (mTORC1) has been implicated in regulating the expansion and differentiation of osteoblasts. Thus, we raise a hypothesis that mTORC1 signaling in osteoblasts plays a vital role in B-cell development. Inactivation of mTORC1 in osterix-expressing cells (mainly osteoblast lineage) through Osx-Cre-directed deletion of Raptor (an mTORC1-specific component) resulted in a reduction in the total B-cell population in the BM, which was due to a block in early B-cell development from the pro-B to pre-B cell stage. Further mechanistic studies revealed that this defect was the result of reduction of interleukin-7 (IL-7) expression in osterix-expressing immature osteoblasts, which caused the abnormality of IL-7/Stat5 signaling in early B lymphocytes, leading to an increased apoptosis of pre-B plus immature B cells. In vitro and in vivo studies demonstrated that the addition of exogenous IL-7 partially restored B lymphopoiesis in the BM of Raptor mutant mice. Furthermore, total BM cells cultured in conditioned media from Raptor null immature osteoblasts or media with anti-IL-7 neutralizing antibody failed to differentiate into pre-B and immature B cells, indicating that inactivation of mTORC1 in immature osteoblast cannot fully support normal B-cell development. Taken together, these findings demonstrate a novel role for mTORC1 in the regulation of bone marrow environments that support B-cell differentiation via regulating IL-7 expression. © 2017 American Society for Bone and Mineral Research.
The effect of postnatal manganese exposure on the NMDA receptor signaling pathway in rat hippocampus
Authors: Wang Lei , Fu HuanHuan , Liu Bin , Liu XiaoYan , Chen WeiWei , Yu XiaoDan 
Published: December 2017

AbstractOverexposure to manganese (Mn) is associated with neurological disorders in children. Evidence indicated that N‐methyl‐d‐aspartate (NMDA) receptor signaling pathway was critical for neurobehavioral function. However, whether NMDA receptor signaling pathway contributes to Mn‐induced neurotoxicity remains unknown. In this study, newborn Sprague–Dawley rats were randomly assigned to four groups exposed to 0, 10, 20, and 30 mg/kg of Mn2+ by intraperitoneal injection (n = 10/group: five males and five females). After 3 weeks of Mn exposure, messenger RNA (mRNA) and protein expression of NMDA receptor subunits (NR1, NR2A, and NR2B), cAMP‐response element binding protein (CREB), and brain‐derived neurotrophic factor (BDNF) in hippocampus were measured by real‐time quantitative RT‐PCR and Western blot. In Mn‐exposed rats, decreased mRNA and protein expression of NR1, NR2A, and NR2B, CREB, and BDNF was observed. The results imply that downregulated NMDA receptor signaling pathway may be of vital importance in the neuropathological process of Mn‐induced neurotoxicity.
Selection of antioxidants against ovarian oxidative stress in mouse model
Authors: Li Bojiang , Weng Qiannan , Liu Zequn , Shen Ming , Zhang Jiaqing , Wu Wangjun , Liu Honglin 
Published: December 2017

AbstractOxidative stress (OS) plays an important role in the process of ovarian granulosa cell apoptosis and follicular atresia. The aim of this study was to select antioxidant against OS in ovary tissue. Firstly, we chose the six antioxidants and analyzed the reactive oxygen species (ROS) level in the ovary tissue. The results showed that proanthocyanidins, gallic acid, curcumin, and carotene decrease the ROS level compared with control group. We further demonstrated that both proanthocyanidins and gallic acid increase the antioxidant enzymes activity. Moreover, change in the ROS level was not observed in proanthocyanidins and gallic acid group of brain, liver, spleen, and kidney tissues. Finally, we found that proanthocyanidins and gallic acid inhibit pro‐apoptotic genes expression in granulosa cells. Taken together, proanthocyanidins and gallic acid may be the most acceptable and optimal antioxidants specifically against ovarian OS and also may be involved in the inhibition of granulosa cells apoptosis in mouse ovary.
Group divisible covering designs with block size four
Authors: Wei Hengjia , Ge Gennian , Colbourn Charles J. 
Published: December 2017

AbstractGroup divisible covering designs (GDCDs) were introduced by Heinrich and Yin as a natural generalization of both covering designs and group divisible designs. They have applications in software testing and universal data compression. The minimum number of blocks in a k‐GDCD of type is a covering number denoted by . When , the values of have been determined completely for all possible pairs . When , Francetić et al. constructed many families of optimal GDCDs, but the determination remained far from complete. In this paper, two specific 4‐IGDDs are constructed, thereby completing the existence problem for 4‐IGDDs of type . Then, additional families of optimal 4‐GDCDs are constructed. Consequently the cases for whose status remains undetermined arise when and , when and , and in several small families for which one of g and u is fixed.
Ethyl chloride spray for musculoskeletal ultrasound‐guided injections: An alternative to subcutaneous injection of local anesthetic solution
Authors: Shah Amit , Vidoni Alessandro , McGarry Sharon , Davies Mark , James Steven , Botchu Rajesh 
Published: December 2017

AbstractUltrasound‐guided injections such as steroid injections are common procedures involving the musculoskeletal system. They are usually performed after a subcutaneous injection of local anesthetic (LA), which can be painful. In 126 consecutive patients, local anesthesia was performed using ethyl chloride spray prior to a therapeutic ultrasound‐guided injection in joints, tendons, or bursae. Ninety‐nine (78.5%) patients found the use of ethyl chloride spray helpful. The use of ethyl chloride spray is an effective, patient‐friendly alternative to the standard injection of local aesthetic for ultrasound‐guided therapeutic musculoskeletal injections with the advantage of a lower cost of $0.18 per procedure.
Mechanisms underlying maternal care in a viviparous snake, Sistrurus miliarius: Does maternal body condition, corticosterone, or arginine vasotocin mediate post‐birth maternal investment?
Authors: Lind Craig M. , Ramis Fatima , Paredes Donna‐Jael G. , Moore Ignacio T. , Vernasco Ben J. , Farrell Terence M. 
Published: December 2017

AbstractThe level of parental investment in free‐living offspring varies greatly within and among vertebrate taxa, and the mechanisms that mediate investment are likely targets of selection in the evolution of reproductive strategies. In mammalian and avian systems, individual investment is adjusted according to parental energetic status, and regulatory mechanisms have been described. Parental care is also a component of the life history of several groups of squamates and archosaurs, but little to nothing is known regarding the mechanisms that underlie individual variation in parental investment in these groups. Therefore, we examined the relationships among maternal body condition, corticosterone, arginine vasotocin (AVT), and maternal care in postparturient pigmy rattlesnakes. Energetic status was manipulated in pregnant females by supplemental feeding, and AVT was manipulated in postparturient mothers by intraperitoneal injection. Circulating corticosterone and body condition were recorded in postparturient mothers. Mother‐offspring spatial relationships were analyzed to determine the closeness of mother‐offspring associations. No significant relationship between maternal body condition and baseline corticosterone was observed in mothers, and no significant relationship was found between corticosterone and the level of maternal association with offspring. Manipulation of AVT did not stimulate maternal care. There was a strong and repeatable positive relationship between maternal postparturient body condition and the level of maternal association with offspring. The lack of a significant correlation between body condition and corticosterone does not support the hypothesis that a postparturient glucocorticoid response drives the observed condition‐dependence of maternal offspring association.
Multigraphs without large bonds are wqo by contraction
Authors: Kamiński Marcin , Raymond Jean‐Florent , Trunck Théophile 
Published: December 2017

AbstractWe show that the class of multigraphs with at most p connected components and bonds of size at most k is well‐quasi‐ordered by edge contraction for all positive integers . (A bond is a minimal nonempty edge cut.) We also characterize canonical antichains for this relation.
Automated production of [18F]FTHA according to GMP
Authors: Savisto Nina , Viljanen Tapio , Kokkomäki Esa , Bergman Jörgen , Solin Olof 
Published: December 2017

14‐(R,S)‐[18F]fluoro‐6‐thia‐heptadecanoic acid is a tracer for fatty acid imaging by positron emission tomography. High demand for this tracer required us to replace semiautomatic synthesis with a fully automated procedure. An automated synthesis device was constructed in‐house for multistep nucleophilic 18F‐fluorination and a control system was developed. The synthesis device was combined with a sterile filtration unit and both were qualified. 14‐(R,S)‐[18F]fluoro‐6‐thia‐heptadecanoic acid was produced according to good manufacturing practice guidelines set by the European Union. The synthesis includes an initial nucleophilic labelling reaction, deprotection, preparative HPLC separation, purification of the final product, and formulation for injection. The duration and temperature of the reaction and hydrolysis were optimized, and the radiochemical stability of the formulated product was determined. The rotary evaporator used to evaporate the solvent after HPLC purification was replaced with solid phase extraction purification. We also replaced the human serum albumin used in the earlier procedure with a phosphate buffer‐ascorbic acid mixture in the final formulation solution. From 2011 to 2016, we performed 219 synthesis procedures, 94% of which were successful. The radiochemical yield of 14‐(R,S)‐[18F]fluoro‐6‐thia‐heptadecanoic acid, decay‐corrected to the end of bombardment, was 13% ± 6.3%. The total amount of formulated end product was 1.7 ± 0.8 GBq at end of synthesis.
Statistical analysis of wheat under different seed treatments: development of a discriminative model based on physicochemical and rheological properties
Authors: Molotto Luiz Antonio , Scheres Firak Daniele , Lagner da Silveira Estevão Priscila , Boger Beatriz , Nagata Noemi , Peralta‐Zamora Patricio , Garabeli Trojan Daiane 
Published: December 2017

AbstractBACKGROUNDQuality control in the wheat industry comprises numerous analyses that are time‐consuming and demand numerous procedures and specific apparatus. The application of multivariate calibration techniques contributes to the interpretation of the data generated during these analyses. The present study aimed to correlate a representative number of wheat properties with the treatment applied to the wheat seeds using multivariate calibration techniques.RESULTSIn the present study, a wheat pilot planting experiment applying different fungicides combination as a seed treatment (carbendazim, carbendazim + thiram, carboxin + thiram, and triadimenol) was conducted. The resulting wheat grains were subjected to 33 analyses routinely performed in industry. A principal components analysis indicated all analyses were relevant for the different seed treatment discrimination. Afterwards, a k‐nearest neighbors discriminative model was developed and was able to classify the seed treatments. In accordance with this model, the most relevant variables for the seed treatment discrimination were the rheological properties of the dough.CONCLUSIONIt was possible to develop a discriminative model that directly correlated the wheat seed treatment with the properties of the resulting grains and flours. © 2017 Society of Chemical Industry
Prognostic impact of postoperative pulmonary complications following salvage esophagectomy after definitive chemoradiotherapy
Authors: Hayami Masaru , Watanabe Masayuki , Ishizuka Naoki , Mine Shinji , Imamura Yu , Okamura Akihiko , Kurogochi Takanori , Yamashita Kotaro 
Published: December 2017

BackgroundPostoperative complications after esophagectomy for esophageal cancer have a negative effect on patients’ survival. Although postoperative complications are more frequently observed after salvage esophagectomy than after planned esophagectomy, the effects of postoperative complications on long‐term oncologic outcomes after salvage esophagectomy remain unclear.MethodsThis retrospective study of 70 esophageal cancer patients after definitive chemoradiotherapy (dCRT) compared long‐term outcomes between those with and without complications. The association between morbidity and overall survival (OS) was evaluated by a Cox regression analysis. To identify the risk factors for pulmonary complications, logistic regression analysis was carried out.ResultsPostoperative complications occurred in 42 (60.0%) patients. Pulmonary complications and anastomotic leakage occurred in 23 (32.9%) and 9 (12.9%) patients, respectively. Overall complications and anastomotic leakage did not affect long‐term outcomes. Survival was significantly worse for patients with pulmonary complications. Radiation dose (<60 Gy), response to dCRT (complete), ypStage (0‐II), residual disease (R0), and pulmonary complications (negative) were independent factors related to a favorable OS. BMI (<20 kg/m2), ASA‐PS (2‐3), and radiation dose (>60 Gy) were significant factors affecting the occurrence of pulmonary complications.ConclusionsDevelopment of postoperative pulmonary complications was independently associated with poor prognosis in patients who underwent salvage esophagectomy after dCRT.
Characteristics and long‐term survival of patients diagnosed with pure tubular carcinoma of the breast
Authors: Poirier Éric , Desbiens Christine , Poirier Brigitte , Boudreau Dominique , Jacob Simon , Lemieux Julie , Doyle Catherine , Diorio Caroline , Hogue Jean‐Charles , Provencher Louise 
Published: December 2017

Background and ObjectivesPure tubular carcinomas (TC) of the breast are generally considered to have an excellent prognosis. This study aimed to analyze the characteristics and survival of patients with TC.MethodsThis was a retrospective study conducted at the CHU de Québec—Université Laval. Databases were searched for all cases treated between April 1997 and December 2010. Survival was retrieved from the Province of Quebec Ministry of Health. Follow‐up was censored on December 31, 2011. Overall survival (OS) was compared to patients with invasive ductal carcinoma (ICD) matched for age, tumor size, lymph node involvement, year of diagnosis, ER, PgR, and HER2, histological grade, lymphovascular invasion, and chemotherapy.ResultsThe frequency of TC was 2.9% (n = 223/7563). Tumors size was 7.4 ± 8.8 mm, without lymphovascular invasion (95.1%), ER‐positive (98.2%), PgR‐positive (69.5%), and HER2‐negative (100%). Patients were followed up for 7.1 ± 2.7 years. The actuarial 13‐year OS was 89.0% for TC, compared to 85.8% for IDC (P = 0.13). For TC, the 13‐year OS was 95.8% in NO patients compared to 90.0% for N1‐3 (P = 0.01).ConclusionDespite the general popular belief that patients with TC fare better than patients with IDC, the 13‐year OS of TC was similar to that of grade I IDC.
Oncologic effects of preoperative biliary drainage in resectable hilar cholangiocarcinoma: Percutaneous biliary drainage has no adverse effects on survival
Authors: Zhang Xu‐Feng , Beal Eliza W. , Merath Katiuscha , Ethun Cecilia G. , Salem Ahmed , Weber Sharon M. , Tran Thuy , Poultsides George , Son Andre Y. , Hatzaras Ioannis , Jin Linda , Fields Ryan C. , Weiss Matthew , Scoggins Charles , Martin Robert C.G. , Isom Chelsea A. , Idrees Kamron , Mogal Harveshp D. , Shen Perry , Maithel Shishir K. , Schmidt Carl R. , Pawlik Timothy M. 
Published: December 2017

Background and ObjectivesThe objective of the current study was to define long‐term survival of patients with resectable hilar cholangiocarcinoma (HCCA) after preoperative percutaneous transhepatic biliary drainage (PTBD) versus endoscopic biliary drainage (EBD).MethodsBetween 2000 and 2014, 240 patients who underwent curative‐intent resection for HCCA were identified at 10 major hepatobiliary centers. Postoperative morbidity and mortality, as well as disease‐specific survival (DSS) and recurrence‐free survival (RFS) were analyzed among patients.ResultsThe median decrease in total bilirubin levels after biliary drainage was similar comparing PTBD (n = 104) versus EBD (n = 92) (mg/dL, 4.9 vs 4.9, P = 0.589) before surgery. There was no difference in baseline demographic characteristics, type of surgical procedure performed, final AJCC tumor stage or postoperative morbidity among patients who underwent EBD only versus PTBD (all P > 0.05). Patients who underwent PTBD versus EBD had a comparable long‐term DSS (median, 43.7 vs 36.9 months, P = 0.802) and RFS (median, 26.7 vs 24.0 months, P = 0.571). The overall pattern of recurrence relative to regional or distant disease was also the same among patients undergoing PTBD and EBD (P = 0.669)ConclusionsOncologic outcomes including DSS and RFS were similar among patients who underwent PTBD versus EBD with no difference in tumor recurrence location.