bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cook Cervical Ripening Balloon successfully prevents excessive hemorrhage combined with ultrasound‐guided suction curettage in the treatment of cesarean scar pregnancy
Authors: Wu Chaoying , Li Yong , Ye Wenfeng , Ma Wenqin , Zhao Danmei 
Published: May 2017

AbstractAimCesarean scar pregnancy (CSP) is a rare occurrence of ectopic pregnancy. CSP incidence has increased significantly as a result of the increase in cesarean section rates. At present, there are no standard treatment guidelines for CSP; therefore, we report a minimally invasive treatment method for patients diagnosed with CSP.MethodsThis study included 15 women who were diagnosed with CSP. Ultrasound‐guided suction curettage was performed on all patients. The Cook Cervical Ripening Balloon was used to tamponade and prevent hemorrhage during the procedure. In 12 patients, the balloon was placed immediately following ultrasound‐guided suction curettage; in two patients, the balloon was placed when excessive bleeding occurred post‐curettage; and in one patient, the balloon was placed after the gestational sac evacuated by itself, and then suction aspiration was performed on day 5, following the evacuation. Human chorionic gonadotropin levels were evaluated three days after the procedure.ResultsPlacement and inflation of the Cook Cervical Ripening Balloon was well tolerated by all patients. The balloon tamponade effectively reduced or prevented vaginal bleeding in all patients, and none of the patients had an estimated blood loss higher than 1000 ml.ConclusionsUltrasound‐guided suction curettage is effective in the treatment of CSP. The Cook Cervical Ripening Balloon is easy to place and inflate and successfully prevented bleeding or assisted in the management of bleeding complications. We recommend the Cook Cervical Ripening Balloon as an adjuvant method for ultrasound‐guided suction curettage for the treatment of CSP.
Real‐time three‐dimensional jaw tracking in temporomandibular disorders
Authors: da Cunha D. V. , Degan V. V. , Vedovello Filho M. , Bellomo D. P. , Silva M. R , Furtado D. A. , Andrade A. O. , Milagre S. T. , Pereira A. A. 
Published: May 2017

SummaryWhen a dysfunction occurs in any component of the stomatognathic system, temporomandibular disorders (TMD) may originate. The aim of this study was to compare the deviations, displacement and the execution speed of mandibular movements among asymptomatic participants and those with TMD. Convenience sampling was used; forty participants diagnosed by clinical evaluation following the Research Diagnostic Criteria for Temporomandibular Disorders were divided into three groups: arthropathy (GART, 10 participants, 40% men), myopathy (GMYO, 10 participants, 30% men), and the control group (CG, 20 asymptomatic participants, 25% men). Participants were asked to perform the movements of free maximal mouth opening and closing, right and left lateral excursions, and protrusion with sliding teeth contacts. The mandibular trajectory was recorded using opto‐electronic devices tracking reflective markers placed in front of the ‘soft tissue pogonion point’. The movements were analysed on the following axis: x – medial‐lateral, y – vertical, z – antero‐posterior. Significative differences were found in CGxGART – unassisted maximal mouth opening and closing projection on y‐axis (OCY), CGxGMYO – unassisted maximal mouth opening and closing projection on x‐axis (OCX), and in the measures Opening lateral deviation on x‐axis (OLDX), closing lateral deviation on x‐axis (CLDX) and in the measures of speed for both. In regard to GARTxGMYO, a significative difference was found in Protrusion lateral deviation on x‐axis (PLDX) ‘Conover‐Iman Test of Multiple Comparisons Using Rank Sums’ using Bonferroni correction (P < 0·05). In conclusion, the total opening movements in individuals with TMD tended to have higher deviation than in those asymptomatic individuals and a reduction in the speed of movements.
Development of a sequence‐characterized amplified region marker using inter‐simple sequence repeats for detection of Puccinia striiformis f. sp. tritici
Authors: Wang Shuhe , Chu Bingyao , Liu Qi , Luo Yong , Ma Zhanhong 
Published: May 2017

AbstractStripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is the most devastating wheat disease in China. Early and accurate detection of the pathogens would facilitate effective control of the diseases. DNA‐based methods now provide essential tools for accurate plant disease diagnosis. In this study, inter‐simple sequence repeats (ISSR) technique has been successfully applied to develop a sequence‐characterized amplified region (SCAR) marker for diagnosis of stripe rust of wheat and detection of Pst. In this study, one fragment unique to Pst was identified by ISSR and then sequenced. Based on the specific fragment, a pair of SCAR primers (616AF/616AR) was designed to amplify a 299‐bp DNA fragment within the sequenced region. The primers can amplify a unique DNA fragment for all tested isolates of Pst but not for the other pathogens of wheat leaves and the uninfected leaves. The polymerase chain reaction (PCR) assay could detect as low as 0.1 ng of genomic DNA in a 25.0 μl PCR reaction mixture and detect the pathogen from asymptomatic wheat leaves inoculated with Pst under glasshouse conditions.
Higher risk of hepatocellular carcinoma in chronic hepatitis B vs chronic hepatitis C after achievement of virologic response
Authors: Kim G.‐A. , Han S. , Kim H.‐D. , An J. , Lim Y.‐S. 
Published: May 2017

SummaryIt is unclear whether the achievement of virologic response modifies the risk of hepatocellular carcinoma (HCC) differently in chronic hepatitis B (CHB) and chronic hepatitis C (CHC). Our aim was to compare the risk of HCC between patients with CHB and CHC who achieved virological response. We analysed data from patients with CHB treated with entecavir (n=2000) or CHC treated with peg‐interferon and ribavirin (n=733) at a tertiary hospital from 2004 to 2011. Virological response was defined as serum HBV DNA<15 IU/mL at 1 year of treatment for CHB or the achievement of sustained virologic response for CHC. Virological response was achieved in 1520 patients with CHB (76.0%) and 475 patients with CHC (64.8%). During the median follow‐up period of 6 years, 228 patients with CHB (11.4%) and 59 patients with CHC (8.0%) developed HCC. Among patients with virological response, CHB was independently associated with a significantly higher incidence of HCC (hazard ratio, 2.17; 95% CI, 1.30‐3.63; P=.003) than CHC. Among patients without virological response, there were no differences in HCC incidence between the two cohorts (P=.52). In patients with cirrhosis at baseline, the incidence of HCC did not differ between the two cohorts even after achieving virological response (P>.99). In conclusion, patients with CHB treated with entecavir were associated with a higher risk of HCC compared to patients with CHC treated with peg‐interferon and ribavirin after achieving virological response. However, the risk of HCC did not differ between the two cohorts if the patients had cirrhosis at baseline, even if virological response was achieved.
Improving outcome of fungal diseases – Guiding experts and patients towards excellence
Authors: Cornely Oliver A. , Lass‐Flörl Cornelia , Lagrou Katrien , Arsic‐Arsenijevic Valentina , Hoenigl Martin 
Published: May 2017

SummaryInvasive fungal infections are on the rise and during recent years understanding the epidemiology of fungal infections improved. Over 1 billion people are affected and 25 million patients are at imminent risk of severe organ damage or death due to fungal infection. The European Confederation of Medical Mycology (ECMM), founded in 1993, is the roof organisation of 23 National Medical Mycology Societies in Europe. ECMM fights fungal infections at various levels, by creating and distributing scientific knowledge and promoting scientific exchange. In response to the increasing prevalence and management complexity of invasive fungal infections, ECMM recently launched three additional initiatives (https://www.ecmm.info/). (i) ECMM together with other European infectious diseases societies created a comprehensive set of European guidelines for the diagnostic and therapeutic management of invasive fungal infections. (ii) ECMM founded the ECMM Academy awarding fellow status (FECMM) to outstanding researchers who advanced medical mycology. The academy aims at strengthening networking activities between these researchers. (iii) Centres throughout the world can apply for ECMM Excellence Center Status. Following such application on site auditing of up to three levels of mycological work (clinical, microbiological, epidemiological/clinical trials) evaluates the excellence of a centre along predefined criteria. All three initiatives share a common ambition; they aim at improving outcome of fungal diseases through guiding experts and patients towards excellence. Acknowledging fungal infections as a global problem, all three initiatives explicitly reach out beyond European borders.
Arabidopsis ZED1‐related kinases mediate the temperature‐sensitive intersection of immune response and growth homeostasis
Authors: Wang Zhicai , Cui Dayong , Liu Jing , Zhao Jingbo , Liu Cheng , Xin Wei , Li Yuan , Liu Na , Ren Dongtao , Tang Dingzhong , Hu Yuxin 
Published: May 2017

Summary

Activation of the immune response in plants antagonizes growth and development in the absence of pathogens, and such an autoimmune phenotype is often suppressed by the elevation of ambient temperature. However, molecular regulation of the ambient temperature‐sensitive intersection of immune response and growth is largely elusive.

A genetic screen identified an Arabidopsis mutant, zed1‐D, by its high temperature‐dependent growth retardation. A combination of molecular, cytological and genetic approaches was used to investigate the molecular basis behind the temperature‐sensitive growth and immune response in zed1‐D.

A dominant mutation in HOPZ‐ETI‐DEFICIENT 1 (ZED1) is responsible for a high temperature‐dependent autoimmunity and growth retardation in zed1‐D. The autoimmune phenotype in zed1‐D is dependent on the HOPZ‐ACTIVATED RESISTANCE 1 (ZAR1). ZED1 and some ZED1‐related kinases (ZRKs) are induced by elevated temperature and function cooperatively to suppress the immune response by modulating the transcription of SUPPRESSOR OF NPR1‐1 CONSTITUTIVE 1 (SNC1) in the absence of pathogens.

Our data reveal a previously unidentified role of ZRKs in the ambient temperature‐sensitive immune response in the absence of pathogens, and thus reveals a possible molecular mechanism underlying the temperature‐mediated intersection of immune response and growth in plants.
Person‐centred care dialectics—Inquired in the context of palliative care
Authors: Öhlén Joakim , Reimer‐Kirkham Sheryl , Astle Barbara , Håkanson Cecilia , Lee Joyce , Eriksson Marjukka , Sawatzky Richard 
Published: May 2017

AbstractAlthough a widely used concept in health care, person‐centred care remains somewhat ambiguous. In the field of palliative care, person‐centred care is considered a historically distinct ideal and yet there continues to be a dearth of conceptual clarity. Person‐centred care is also challenged by the pull of standardization that characterizes much of health service delivery. The conceptual ambiguity becomes especially problematic in contemporary pluralistic societies, particularly in the light of continued inequities in healthcare access and disparities in health outcomes. Our aim was to explicate premises and underlying assumptions regarding person‐centred care in the context of palliative care with an attempt to bridge the apparently competing agendas of individualization versus standardization, and individuals versus populations. By positioning person‐centredness in relation to the hermeneutics of the self according to Paul Ricœur, dialectics between individualization and standardization, and between individuals and populations were constructed. The competing agendas were related in a dialectic manner in the way that population health is of importance for the individual, and standardization is of importance for the population. The analysis suggests that person‐centred care is an ethical stance, which gives prominence to both suffering and capability of the individual as a person. The dialectic analysis points towards the importance of extending person‐centred care to encompass population and societal perspectives and thereby avoiding a problematic tendency of affiliating person‐centred care with exclusively individualistic perspectives. Considerations for person‐centred palliative care on micro‐, meso‐ and macrolevels conclude the paper.
MutMapPlus identified novel mutant alleles of a rice starch branching enzyme IIb gene for fine‐tuning of cooked rice texture
Authors: Nakata Masaru , Miyashita Tomomi , Kimura Rieko , Nakata Yuriko , Takagi Hiroki , Kuroda Masaharu , Yamaguchi Takeshi , Umemoto Takayuki , Yamakawa Hiromoto 
Published: May 2017

SummaryPhysicochemical properties of storage starch largely determine rice grain quality and food characteristics. Therefore, modification of starch property is effective to fine‐tune cooked rice textures. To obtain new resources with modified starch property as breeding materials, we screened a mutant population of a japonica cultivar Nipponbare and found two independent mutant lines, altered gelatinization (age)1 and age2, with moderate changes in starch gelatinization property. A combination of conventional genetic analyses and the latest mapping method, MutMapPlus, revealed that both of these lines harbour novel independent mutant alleles of starch branching enzyme IIb (BEIIb) gene. In age1, amino acid substitution of Met‐723 to Lys completely abolished BEIIb enzyme activity without significant reduction in its protein level. A transposon insertion in an intron of BEIIb gene reduced BEIIb protein level and activity in age2. Production of a series of the mutant lines by combining age alleles and indica‐type starch synthase IIa allele established stepwise alteration of the physicochemical properties of starch including apparent amylose content, thermal property, digestibility by α‐amylase and branched structures of amylopectin. Consistent with the alteration of starch properties, the results of a sensory evaluation test demonstrated that warm cooked rice of the mutants showed a variety of textures without marked reduction in overall palatability. These results suggest that a series of the mutant lines are capable of manipulation of cooked rice textures.
High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
Authors: Wang Pengcheng , Zhang Jun , Sun Lin , Ma Yizan , Xu Jiao , Liang Sijia , Deng Jinwu , Tan Jiafu , Zhang Qinghua , Tu Lili , Daniell Henry , Jin Shuangxia , Zhang Xianlong 
Published: May 2017

SummaryGossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity is also very high between gene homologues. To efficiently achieve site/gene‐specific mutation is quite needed. Due to its high efficiency and robustness, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system has exerted broad site‐specific genome editing from prokaryotes to eukaryotes. In this study, we utilized a CRISPR/Cas9 system to generate two sgRNAs in a single vector to conduct multiple sites genome editing in allotetraploid cotton. An exogenously transformed gene Discosoma red fluorescent protein2(DsRed2) and an endogenous gene GhCLA1 were chosen as targets. The DsRed2‐edited plants in T0 generation reverted its traits to wild type, with vanished red fluorescence the whole plants. Besides, the mutated phenotype and genotype were inherited to their T1 progenies. For the endogenous gene GhCLA1, 75% of regenerated plants exhibited albino phenotype with obvious nucleotides and DNA fragments deletion. The efficiency of gene editing at each target site is 66.7–100%. The mutation genotype was checked for both genes with Sanger sequencing. Barcode‐based high‐throughput sequencing, which could be highly efficient for genotyping to a population of mutants, was conducted in GhCLA1‐edited T0 plants and it matched well with Sanger sequencing results. No off‐target editing was detected at the potential off‐target sites. These results prove that the CRISPR/Cas9 system is highly efficient and reliable for allotetraploid cotton genome editing.
Inducing unconscious stress: Cardiovascular activity in response to subliminal presentation of threatening and neutral words
Authors: van der Ploeg Melanie M. , Brosschot Jos F. , Verkuil Bart , Gillie Brandon L. , Williams DeWayne P. , Koenig Julian , Vasey Michael W. , Thayer Julian F. 
Published: May 2017

AbstractStress‐related cognitive processes may occur outside of awareness, here referred to as unconscious stress, and affect one's physiological state. Evidence supporting this idea would provide necessary clarification of the relationship between psychological stress and cardiovascular (CV) health problems. We tested the hypothesis that increases in mean arterial pressure (MAP) and total peripheral resistance (TPR) and decreases in heart rate variability (HRV) would be larger when threatening stimuli are presented outside of awareness, or subliminally, compared with neutral stimuli. Additionally, it was expected that trait worry and resting HRV, as common risk factors for CV disease, would moderate the effect. We presented a subliminal semantic priming paradigm to college students that were randomly assigned to the threat (n = 56) or neutral condition (n = 60) and assessed changes from baseline of MAP, TPR, and HRV. Level of trait worry was assessed with the Penn State Worry Questionnaire. The findings indicate that CV activity changed according to the hypothesized pattern: A higher MAP and TPR and a lower HRV in the threat condition compared with the neutral condition were found with practically meaningful effect sizes. However, these findings were only statistically significant for TPR. Furthermore, changes in CV activity were not moderated by trait worry or resting HRV. This is the first study to explicitly address the role of subliminally presented threat words on health‐relevant outcome measures and suggests that unconscious stress can influence peripheral vascular resistance.
Health promotion for people with intellectual disabilities – A concept analysis
Authors: Roll Anne E. 
Published: May 2017

BackgroundWhereas ‘health promotion’ is a well‐known concept for healthcare professionals, the concept of ‘health promotion for people with intellectual disabilities’ and its unique associated challenges are not well understood.Aims and ObjectiveThis article provides a systematic analysis of how health promotion is being conceptualised for people with intellectual disabilities and how health promotion can work best in the light of this group's specific needs and limitations.DesignRodgers' evolutionary concept analysis.MethodMEDLINE, PsycINFO, CINAHL and SocINDEX were searched using the search terms ‘health promotion’, ‘people with intellectual disabilities’ and ‘developmental disabilities’. This review includes studies published between 1992 and 2014. A total of 52 articles were included.ResultsHealth promotion for people intellectual disabilities, as discussed in the literature, focuses on four aspects, namely supporting a healthy lifestyle, providing health education, involving supporters and being person‐centred. Antecedents of the concept ‘health promotion for people with intellectual disabilities’ were healthcare access and sensitised healthcare providers. The outcomes were improved health, being empowered, enhanced quality of life and reduced health disparities.ConclusionThis analysis provides a solid foundation for healthcare stakeholders' planning, implementing and evaluating health‐promotion activities for people with intellectual disabilities at the policy level and in the community.
Metagenomics analysis of red blood cell and fresh‐frozen plasma units
Authors: Lau Pierre , Cordey Samuel , Brito Francisco , Tirefort Diderik , Petty Thomas J. , Turin Lara , Guichebaron Arthur , Docquier Mylène , Zdobnov Evgeny M. , Waldvogel‐Abramowski Sophie , Lecompte Thomas , Kaiser Laurent , Preynat‐Seauve Olivier 
Published: May 2017

BACKGROUNDAlthough the risk of transmitting infectious agents by blood transfusion is dramatically reduced after donor selection, leukoreduction, and laboratory testing, some could still be present in donor's blood. A description of metagenomes in blood products eligible for transfusion represents relevant information to evaluate the risk of pathogen transmission by transfusion.STUDY DESIGN AND METHODSDetection of viruses, bacteria, and fungi genomes was made by high‐throughput sequencing (HTS) of 600 manufactured blood products eligible for transfusion: 300 red blood cell (RBC) and 300 fresh‐frozen plasma (FFP) units.RESULTSAnelloviruses and human pegivirus, frequent in the blood of healthy individuals, were found. Human papillomavirus type 27 and Merkel cell polyomavirus, present on the skin, were also detected. Unexpectedly, astrovirus MLB2 was identified and characterized in a FFP unit. The presence of astrovirus MLB2 was confirmed in donor's blood and corresponded to an asymptomatic acute viremia. Sequences of bacteria and fungi were also detected; they are likely the result of environmental contamination.CONCLUSIONThis study demonstrates that HTS is a promising tool for detecting common and less frequent infectious pathogens in blood products
Differences in growth and herbicide sensitivity among Cyperus esculentus clones found in Belgian maize fields
Authors: De Cauwer B , De Ryck S , Claerhout S , Biesemans N , Reheul D 
Published: May 2017

SummaryCyperus esculentus is an invasive troublesome neophyte in many arable crops in Belgium. Applied weed control varies from field to field. One of the possible reasons for this variability might be a differential vegetative and reproductive behaviour among Belgian C. esculentus clones. In this study, growth characteristics and herbicide sensitivity of C. esculentus clones collected in Belgian maize (Zea mays) fields were evaluated. In a morphology Experiment, 25 clones were screened for growth characteristics and ability to set viable seeds under outdoor conditions. Dose–response experiments were conducted in the glasshouse to evaluate the effectiveness of two foliar‐applied herbicides (bentazon and glyphosate) and two pre‐sowing soil‐incorporated herbicides (S‐metolachlor and dimethenamid‐P) for controlling 14 C. esculentus clones. Response variables were aboveground dry biomass, tuber number, tuber dry biomass and individual tuber dry weight. Clones exhibited large differences in shoot number (up to 3.1‐fold), tuber dry biomass (up to 4.7‐fold), tuber number (up to 3.4‐fold), individual tuber dry weight (up to 4.8‐fold), inflorescence number and capacity to set viable seeds. Large interclonal differences in herbicide sensitivity (up to 8.3‐ and 4.0‐fold for aboveground dry biomass and tuber dry biomass, respectively) were observed. Contrary to foliar‐applied herbicides, soil‐incorporated herbicides were very effective and provided season‐long C. esculentus control at doses below the recommended maximum field dose. However, low doses stimulated tuber formation. Future C. esculentus management strategies should take into account differential growth characteristics and herbicide sensitivity of C. esculentus clones.