bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post‐translational incorporation of 3,4‐dihydroxyphenylalanine into the C terminus of α‐tubulin in living cells
Authors: Dentesano Yanela M. , Ditamo Yanina , Hansen Cristian , Arce Carlos A. , Bisig Carlos Gaston 
Published: January 2018

The C‐terminal tyrosine (Tyr) of the α‐tubulin chain is subjected to post‐translational removal and readdition in a process termed the “detyrosination/tyrosination cycle”. We showed in previous studies using soluble rat brain extracts that l‐3,4‐dihydroxyphenylalanine (l‐Dopa) is incorporated into the same site as Tyr. We now demonstrate that l‐Dopa incorporation into tubulin also occurs in living cells. We detected such incorporation by determining the “tyrosination state” of tubulin before and after incubation of cells in the presence of l‐Dopa. The presence of a tubulin isospecies following l‐Dopa incubation that was not recognized by antibodies specific to Tyr‐ and deTyr‐tubulin was presumed to reflect formation of Dopa‐tubulin. l‐Dopa was identified by HPLC as the C‐terminal compound bound to α‐tubulin. l‐Dopa incorporation into tubulin was observed in Neuro 2A cells and several other cell lines, and was not due to de novo protein biosynthesis. Dopa‐tubulin had microtubule‐forming capability similar to that of Tyr‐ and deTyr‐tubulin. l‐Dopa incorporation into tubulin did not notably alter cell viability, morphology, or proliferation rate. CAD cells (a neuron‐like cell line derived from mouse brain) are easily cultured under differentiating and nondifferentiating conditions, and can be treated with l‐Dopa. Treatment of CAD cells with l‐Dopa and consequent increase in l‐Dopa‐tubulin resulted in reduction of microtubule dynamics in neurite‐like processes.
Do characteristics of pollinator‐friendly gardens predict the diversity, abundance, and reproduction of butterflies?
Authors: Majewska Ania A. , Sims Stuart , Wenger Seth J. , Davis Andrew K. , Altizer Sonia 
Published: January 2018

AbstractPollinator declines have motivated efforts to plant nectar and host plants for butterflies and other pollinators, but whether gardens promote pollinator conservation requires further investigation.We established garden plots to determine whether plant type (native vs. exotic) and weed maintenance (low or high) influence adult butterfly abundance and species richness, and the abundance and survival of immature stages of four species (monarch, queen, black swallowtail, and gulf fritillary). To assess how predator activity and mortality of immature stages might differ inside gardens compared to other suitable habitat patches, we compared caterpillar survival on sentinel host plants placed within and outside of plots.The abundance and species richness of adult butterflies (all taxa) increased with the number of flowering plants per plot, but did not depend on plant type or weeding treatment. Exotic plots had greater adult monarch and gulf fritillary abundances, greater monarch and queen egg counts, and lower black swallowtail caterpillar counts relative to native plots.The survival of immature stages (egg to instar 5) ranged from 2 to 13% and was similar across plots, except for gulf fritillary larvae, which had higher survival in exotic plots. Monarch caterpillar mortality risk was higher on sentinel plants placed inside plots, relative to those outside of plots.This study suggests that garden plots attracted a diversity of adult butterflies and supported the reproduction of focal species. Given lower immature monarch survival within versus outside of plots, further work is needed to examine natural enemy pressure within pollinator gardens.
Containing influenza outbreaks with antiviral use in long‐term care facilities in Taiwan, 2008‐2014
Authors: Cheng Hao‐Yuan , Chen Wan‐Chin , Chou Yu‐Ju , Huang Angela Song‐En , Huang Wan‐Ting 
Published: January 2018

BackgroundsInfluenza can spread rapidly in long‐term care facilities (LTCFs), and residents are usually at higher risk for influenza infections.ObjectiveOur study aimed to evaluate the effectiveness of antiviral interventions on outbreak control.MethodsTaiwan Centers for Disease Control used a syndromic surveillance system to monitor outbreaks in LTCFs. Local public health authorities verified those outbreaks and logged reports to the Epidemic Investigation Report Files Management System (EIRFMS). We conducted a retrospective cohort study by reviewing EIRFMS reports of influenza outbreaks in LTCFs during 2008‐2014. An influenza outbreak was defined as 3 or more cases of influenza‐like illness occurring within a 48‐hours period with ≥1 case of real‐time RT‐PCR‐confirmed influenza in the same LTCF. Antiviral interventions included providing antiviral treatment for patients and antiviral prophylaxis for contacts during outbreaks.ResultsOf 102 influenza outbreaks, median days from onset of the first patient to outbreak notification was 4 (range 0‐22). Median attack rate was 24% (range 2.2%‐100%). Median influenza vaccination coverage among residents was 81% (range 0%‐100%); 43% occurred during the summer months. Even though antiviral treatment was provided in 87% of the outbreaks, antiviral prophylaxis was implemented in only 40%. Starting antiviral treatment within 2 days of outbreak onset was associated with keeping attack rates at <25% (OR 0.29, 95% CI: 0.12‐0.71).ConclusionsEarly initiation of antiviral treatment may reduce the magnitude of influenza outbreaks. Clinicians should identify patients with influenza and start antiviral use early to prevent large outbreaks in LTCFs.
Constructive controversy and creative process engagement: The roles of positive conflict value, cognitive flexibility, and psychological safety
Authors: Ou Zhanying , Chen Tingting , Li Fuli , Tang Pokman 
Published: January 2018

AbstractThis research explores how and when constructive controversy enhances creative process engagement. We identify positive conflict value and cognitive flexibility as two mediating processes that transmit the effect of constructive controversy on creative process engagement. We further theorize that psychological safety shows opposing moderating effects: It strengthens the positive conflict value–creative process engagement link, whereas weakens the cognitive flexibility–creative process engagement link. Moreover, we propose that psychological safety strengthens and weakens the two mediating pathways through positive conflict value and cognitive flexibility, respectively. Results based on a multiwave data involving 239 employees from the research and development division of a large high‐technology company provide support for our hypotheses. Theoretical and practical implications are discussed.
A retrospective study of feline trauma patients admitted to a referral centre
Authors: Hernon T. , Gurney M. , Gibson S. 
Published: January 2018

ObjectiveTo identify prognostic information and provide recommendations for management of feline trauma patients.Methods
Retrospective analysis of case records for 185 cats presented as emergency trauma cases to a referral hospital between February 2009 and December 2013. Each case was assigned a severity score from 1 (very minor injuries) to 6 (moribund, dying). The data were analysed using Mann–Whitney U and Spearman's rank correlation tests. Logistic regression was used to calculate odds ratios.
Results
Out of 185 cats, 22 (11%) did not survive to discharge. Those presenting with a higher severity score had a higher rate of mortality and a longer period of hospitalisation. Road traffic accidents were the most common cause of trauma (104/185) and had the highest mortality and complication rates. Cats with circulatory shock and multiple injuries were identified as having a higher rate of mortality.
Clinical significance
Cats involved in road traffic accidents and that present with signs of shock or multiple injuries following a traumatic event have an increased mortality rate. Cats with a higher severity score had an increased duration of hospitalisation.
Cannabis use is associated with reduced prevalence of progressive stages of alcoholic liver disease
Authors: Adejumo Adeyinka C. , Ajayi Tokunbo O. , Adegbala Oluwole M. , Adejumo Kelechi L. , Alliu Samson , Akinjero Akintunde M. , Onyeakusi Nnaemeka E. , Ojelabi Ogooluwa , Bukong Terence N. 
Published: January 2018

AbstractBackgroundAbusive alcohol use has well‐established health risks including causing liver disease (ALD) characterized by alcoholic steatosis (AS), steatohepatitis (AH), fibrosis, cirrhosis (AC) and hepatocellular carcinoma (HCC). Strikingly, a significant number of individuals who abuse alcohol also use Cannabis, which has seen increased legalization globally. While cannabis has demonstrated anti‐inflammatory properties, its combined use with alcohol and the development of liver disease remain unclear.AimThe aim of this study was to determine the effects of cannabis use on the incidence of liver disease in individuals who abuse alcohol.MethodsWe analysed the 2014 Healthcare Cost and Utilization Project‐Nationwide Inpatient Sample (NIS) discharge records of patients 18 years and older, who had a past or current history of abusive alcohol use (n = 319 514). Using the International Classification of Disease, Ninth Edition codes, we studied the four distinct phases of progressive ALD with respect to three cannabis exposure groups: non‐cannabis users (90.39%), non‐dependent cannabis users (8.26%) and dependent cannabis users (1.36%). We accounted for the complex survey sampling methodology and estimated the adjusted odds ratio (AOR) for developing AS, AH, AC and HCC with respect to cannabis use (SAS 9.4).ResultsOur study revealed that among alcohol users, individuals who additionally use cannabis (dependent and non‐dependent cannabis use) showed significantly lower odds of developing AS, AH, AC and HCC (AOR: 0.55 [0.48‐0.64], 0.57 [0.53‐0.61], 0.45 [0.43‐0.48] and 0.62 [0.51‐0.76]). Furthermore, dependent users had significantly lower odds than non‐dependent users for developing liver disease.ConclusionsOur findings suggest that cannabis use is associated with a reduced incidence of liver disease in alcoholics.
The Effect of PhD Funding on Postdegree Research Career and Publication Productivity
Authors: Nisticò Roberto 
Published: January 2018

AbstractThis paper explores the extent to which PhD funding encourages postdegree research career and leads to publications. Using novel data on Italian PhD graduates, I find positive modest effects of funding both on pursuing a research career and on publication productivity within a few years after graduation. The results are robust to a battery of checks and different model specifications. I also provide evidence that funded students invest more in research‐oriented activities and spend less time on outside (non‐research) work during the PhD, thus highlighting mechanisms that potentially account for the effect of funding.
Designing a Sanitary Process System
Authors: Pastore Frank 
Published: January 2018

Years ago a sanitary process system could be developed at the corporate and/or plant level by the engineering and operations staff and a few key vendors.
Today most engineering departments in the beverage industry have been drastically reduced in size or completely eliminated making it impossible for a company to complete a process system without outside help. Therefore, it is imperative for the end user to work closely with a recognized process equipment supplier to develop a system not limited to a line of equipment but utilizing complete process system engineering capabilities. This is especially true today as line sizes have increased in size and scope making it near impossible to design and build it in a small fabricating shop.
Prior to discussions with the process equipment supplier, the end user will have defined his or her final objectives.
Paper published with permission.
Overview of Mechanical Harvesting in Florida Citrus
Authors: Brown G. K. 
Published: January 2018

Florida produces more citrus than all other states in the United States. In 1999, the total bearing area was estimated at 315,900 ha, and 245,000 ha were oranges (Anon, 2000). Recently, oranges averaged 77% of the total production and about 95% were processed. Orange production is projected to increase unless US weather, disease, labor, or economic forces act to depress production (Anon, 1993). Worldwide production and price competition in processed oranges are projected to decrease US grower returns, as free-trade conditions progress.
Paper published with permission.
The Power of Insulation in the Citrus Industry
Authors: King Ronald L. 
Published: January 2018

Insulation is a forgotten technology that can provide an unrivaled return on investment. The Citrus Industry is not an exception. Insulation is the ‘Rodney Dangerfield’ of the construction industry: It receives very little respect and is taken for granted. Insulation is a powerful resource when designed, applied, and maintained properly. Yet, this technology is often forgotten or put on the bottom of the list and ignored.
A recent survey conducted by the National Insulation Association of more than 160 industrial plants, manufacturing, engineering, and architectural firms found that:
• Most had no idea of the payback period, rate of return, with the use of insulation, nor a method to quantify costs versus savings
• Many acknowledged that numerous areas of insulation were in serious need of repair
• The majority did not understand that insulation had any real environmental “tie in”
• Some did not consider additional insulation necessary: “the plant is working fine”
• Many could not relate corrosion under insulation to having anything to do with the insulation
• Most acknowledged their specifications were outdated
• Many confirmed they did not have a dedicated job function to address insulation specifications or anyone who was the “insulation champion”
• Many did not think of insulation as a system or requiring any special design review or technical consideration
That survey confirmed the ‘Rodney Dangerfield’ characterization and formed the basis for the foundation of a major industry educational and awareness initiative.
The benefits of insulation are in many cases invisible and long lasting. This technology is not some mysterious myth. Possibly it is misunderstood and under appreciated due to lack of knowledge. Calculating the operational benefits and the return on investment can be relatively simple. However, an insulation system does not have any moving parts, computer chips, or fancy gauges, and it is certainly not sexy. Maybe that is why in many circles insulation is not an exciting topic of discussion, even though it is a time tested and proven technology that can often provide an annual return on investment greater than 100%. There has not been a more important time in recent history than now to think about insulation differently. The Citrus Industry is not immune to that thought process.
Paper published with permission.
Innovative Mechanical Water Treatment for Evaporative Condensers at a Citrus Facility
Authors: Vella Philip 
Published: January 2018

This paper will describe the use of non-chemical technology for the treatment of cooling water from evaporative condensers at a citrus facility. The technology used is Controlled Hydrodynamic Cavitation (CHC). CHC is one of the most innovative technologies employed today and is unlike all other non-chemical technologies currently available that provide scale, corrosion, and microbiological control in addition to water conservation.
The objectives of this study conducted in 2003–2004 were to: (a) provide scale, corrosion, and microbiological control to a state-of-the-art facility which cools one of the largest refrigerated enclosures in North America, (b) improve condenser operating efficiency over the existing condenser systems, (c) conserve water by minimizing condenser makeup, (d) produce a reduced quantity of condenser bleed with reduced levels of contaminants, and (e) implement environmental improvements and worker safety wherever possible.
Based on the data obtained from the study, all objectives were met, operating expenditures were reduced, and the facility successfully continues to use the CHC technology.
Paper published with permission.
2010 Regulatory Update for Anhydrous Ammonia Refrigeration Processes
Authors: Ira Martha Mead 
Published: January 2018

Refrigeration processes that contain more than 10,000 lb of anhydrous ammonia refrigerant are subject to a number of environmental and safety regulations, including the following:
▪ Occupational Safety and Health Administration’s (OSHA’s) Process Safety Management of Highly Hazardous Chemicals (the PSM Standard), 29 CFR 1910.119
▪ Environmental Protection Agency’s (EPA’s) Risk Management Plan (RMP) Rule, 40 CFR, Part 68
▪ EPA’s Emergency Planning and Community Right to Know Act (EPCRA), 40 CFR parts 350 to 372,
▪ Department of Homeland Security’s (DHS’s) Chemical Facility Anti-Terrorism Standard, 6 CFR Part 27.
These regulations require periodic reporting and other actions, such as employee training and the implementation of hazard prevention programs and emergency response plans. This paper provides a brief summary of the listed rules, as they apply to ammonia refrigeration processes, and describes the following regulatory changes and trends:
▪ OSHA 2009 PSM Covered Chemical Facilities National Emphasis Program, which will include a focus on ammonia refrigeration facilities
▪ EPA’s recently implemented RMP e*Submit procedure
▪ Recent changes to the Tier II reporting for Florida facilities
▪ Rulemaking related to the DHS chemical facility antiterrorism standard.
This paper should be helpful to ammonia refrigeration facility owners and operators who are struggling to stay in compliance with the many regulations applicable to their process. Additionally, the references and website links listed on the bibliography of this paper will serve as resources to further assist such owners and operators in the future, as regulations continue to change. All website addresses were checked on February 16, 2010.
Paper published with permission.
Juice Debittering: Basic Science, Optimization, and Recent Advances
Authors: Ghanem Fred 
Published: January 2018

Bitterness such as Naringin in Grapefruits and Limonin in all Citrus fruits have a strong influence on consumers’ choices for their favorite juices. There have been many methods from ultrafiltration to biocatalysis used to lower such bitter compounds and make the juices more desirable by the consumer. One major tool for such debittering operation is the use of synthetic adsorbents which will be discussed in this paper.
Ion exchange resins and adsorbents have been used for over a century in various food applications to concentrate flavors, decolorize juices, and enhance the quality of the final product. These types of resins are being synthesized to specific parameters to distinguish them from other tools.
Mitsubishi Chemical’s work on optimizing their synthetic adsorbents for high bitterness removal from citrus juice was investigated. Parameters such as the base matrix structure, pore size and distribution, as well as the effect of surface area were studied. As the FDA has strict definitions about the appropriate resin chemistry that can be used in a food application (21 CFR 173.65), progress in new resin chemistry was limited by such regulations.
This paper discusses the use of the original Sepabeads SP70 which was introduced into the market about 20 years ago, to the high capacity resin, Sepabeads SP700, which was introduced 10 years ago, and finally, to the Sepabeads SP710, which is the current optimized version of 20 years of research work. Mitsubishi Chemical’s resins were compared to other resins in the industry for the removal of naringin, limonin, and 8-hydroxyfuranocoumarin (furanocoumarins are compounds that affects the proper absorption of certain medications). Proper regeneration and rejuvenation of these resins were outlined.
Paper published with permission.
Population Pharmacokinetics of Finafloxacin in Healthy Volunteers and Patients with Complicated Urinary Tract Infections
Authors: Taubert Max , Lückermann Mark , Vente Andreas , Dalhoff Axel , Fuhr Uwe 
Published: January 2018

ABSTRACT

Finafloxacin is a novel fluoroquinolone with increased antibacterial activity at acidic pH and reduced susceptibility to several resistance mechanisms. A phase II study revealed a good efficacy/safety profile in patients with complicated urinary tract infections (cUTIs), while the pharmacokinetics was characterized by highly variable concentration-versus-time profiles, suggesting the need for an elaborated pharmacokinetic model. Data from three clinical trials were evaluated: 127 healthy volunteers were dosed orally (
n
= 77) or intravenously (
n
= 50), and 139 patients with cUTI received finafloxacin intravenously. Plasma (2,824 samples from volunteers and 414 samples from patients) and urine (496 samples from volunteers and 135 samples patients) concentrations were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). NONMEM was used to build a population pharmacokinetic model, and pharmacokinetic/pharmacodynamic relationships were investigated via simulations and logistic regression. A two-compartment model with first-order elimination described the data best (central volume of distribution [
V
c
] and peripheral volume of distribution [
V
p
] of 47 liters [20%] and 43 liters [67%], respectively, and elimination clearance and intercompartmental clearance of 21 liters/h [54%] and 2.8 liters/h [57%], respectively [median bootstrap estimates {coefficients of variation}]).
V
c
increased with body surface area, and clearance was reduced in patients (−29%). Oral absorption was described best by parallel first- and zero-order processes (bioavailability of 75%). No pharmacodynamic surrogate parameter of clinical/microbiological outcome could be identified, which depended exclusively on the MIC of the causative pathogens. Despite the interindividual variability, the present data set does not support covariate-based dose adjustments. Based on the favorable safety and efficacy data, the clinical relevance of the observed variability appears to be limited. (This study has been registered at ClinicalTrials.gov under identifier NCT01928433.)