bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenic p62/SQSTM1 mutations impair energy metabolism through limitation of mitochondrial substrates
Authors: Bartolome Fernando , Esteras Noemi , Martin-Requero Angeles , Boutoleau-Bretonniere Claire , Vercelletto Martine , Gabelle Audrey , Le Ber Isabelle , Honda Tadashi , Dinkova-Kostova Albena T. , Hardy John , Carro Eva , Abramov Andrey Y. 
Published: May 2017

AbstractAbnormal mitochondrial function has been found in patients with frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Mutations in the p62 gene (also known as SQSTM1) which encodes the p62 protein have been reported in both disorders supporting the idea of an ALS/FTD continuum. In this work the role of p62 in energy metabolism was studied in fibroblasts from FTD patients carrying two independent pathogenic mutations in the p62 gene, and in a p62-knock-down (p62 KD) human dopaminergic neuroblastoma cell line (SH-SY5Y). We found that p62 deficiency is associated with inhibited complex I mitochondrial respiration due to lack of NADH for the electron transport chain. This deficiency was also associated with increased levels of NADPH reflecting a higher activation of pentose phosphate pathway as this is accompanied with higher cytosolic reduced glutathione (GSH) levels. Complex I inhibition resulted in lower mitochondrial membrane potential and higher cytosolic ROS production. Pharmacological activation of transcription factor Nrf2 increased mitochondrial NADH levels and restored mitochondrial membrane potential in p62-deficient cells. Our results suggest that the phenotype is caused by a loss-of-function effect, because similar alterations were found both in the mutant fibroblasts and the p62 KD model. These findings highlight the implication of energy metabolism in pathophysiological events associated with p62 deficiency.
Effect of alkaline microwaving pretreatment on anaerobic digestion and biogas production of swine manure
Authors: Yu Tao , Deng Yihuan , Liu Hongyu , Yang Chunping , Wu Bingwen , Zeng Guangming , Lu Li , Nishimura Fumitake 
Published: May 2017

AbstractMicrowave assisted with alkaline (MW-A) condition was applied in the pretreatment of swine manure, and the effect of the pretreatment on anaerobic treatment and biogas production was evaluated in this study. The two main microwaving (MW) parameters, microwaving power and reaction time, were optimized for the pretreatment. Response surface methodology (RSM) was used to investigate the effect of alkaline microwaving process for manure pretreatment at various values of pH and energy input. Results showed that the manure disintegration degree was maximized of 63.91% at energy input of 54 J/g and pH of 12.0, and variance analysis indicated that pH value played a more important role in the pretreatment than in energy input. Anaerobic digestion results demonstrated that MW-A pretreatment not only significantly increased cumulative biogas production, but also shortened the duration for a stable biogas production rate. Therefore, the alkaline microwaving pretreatment could become an alternative process for effective treatment of swine manure.
Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance
Authors: Rodrigues Tiago , Matafome Paulo , Sereno José , Almeida José , Castelhano João , Gamas Luís , Neves Christian , Gonçalves Sónia , Carvalho Catarina , Arslanagic Amina , Wilcken Elinor , Fonseca Rita , Simões Ilda , Conde Silvia Vilares , Castelo-Branco Miguel , Seiça Raquel 
Published: May 2017

AbstractMicrovascular dysfunction has been suggested to trigger adipose tissue dysfunction in obesity. This study investigates the hypothesis that glycation impairs microvascular architecture and expandability with an impact on insulin signalling. Animal models supplemented with methylglyoxal (MG), maintained with a high-fat diet (HFD) or both (HFDMG) were studied for periepididymal adipose (pEAT) tissue hypoxia and local and systemic insulin resistance. Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was used to quantify blood flow in vivo, showing MG-induced reduction of pEAT blood flow. Increased adipocyte size and leptin secretion were observed only in rats feeding the high-fat diet, without the development of hypoxia. In turn, hypoxia was only observed when MG was combined (HFDMG group), being associated with impaired activation of the insulin receptor (Tyr1163), glucose intolerance and systemic and muscle insulin resistance. Accordingly, the adipose tissue angiogenic assay has shown decreased capillarization after dose-dependent MG exposure and glyoxalase-1 inhibition. Thus, glycation impairs adipose tissue capillarization and blood flow, hampering its expandability during a high-fat diet challenge and leading to hypoxia and insulin resistance. Such events have systemic repercussions in glucose metabolism and may lead to the onset of unhealthy obesity and progression to type 2 diabetes.
Comorbidity burden of patients with Parkinson’s disease and Parkinsonism between 2003 and 2012: A multicentre, nationwide, retrospective study in China
Authors: Wang Xin , Zeng Fan , Jin Wang-Sheng , Zhu Chi , Wang Qing-Hua , Bu Xian-Le , Luo Hong-Bo , Zou Hai-Qiang , Pu Jie , Zhou Zhong-He , Cui Xiao-Ping , Wang Qing-Song , Shi Xiang-Qun , Han Wei , Wu Qiang , Chen Hui-Sheng , Lin Hang , Zhang Li-Li , Zhang Meng , Lian Yan , Xu Zhi-Qiang , Zhou Hua-Dong , Zhang Tao , Wang Yan-Jiang 
Published: May 2017

AbstractParkinson’s disease (PD) and Parkinsonism are common neurodegenerative disorders with continuously increasing prevalence, causing high global burdens. However, data concerning the comorbidity burden of patients with PD or Parkinsonism in China are lacking. To investigate the health condition and comorbidity burden, a total of 3367 PD and 823 Parkinsonism patients were included from seven tertiary hospitals in seven cities across China from 2003 to 2012. Their comorbidity burden was collected and quantified by the Elixhauser Comorbidity Index (ECI) and Charlson Comorbidity Index (CCI). The comorbidity spectra differed between PD and Parkinsonism patients. Compared with PD patients, Parkinsonism patients were older (69.8 ± 11.5 vs. 67.9 ± 11.4, P < 0.001); had a higher comorbidity burden, including ECI (1.1 ± 1.2 vs. 1.0 ± 1.2, P < 0.001) and CCI (1.3 ± 1.6 vs. 1.1 ± 1.5, P < 0.001); and had higher hospitalization expenses. The ECI (1.1 ± 1.3 vs. 0.9 ± 1.1, P < 0.001) and CCI (1.3 ± 1.6 vs. 0.9 ± 1.2, P < 0.001) were higher in males than in females. The average length of stay and daily hospitalization expenses increased with age, as did ECI and CCI. This is the first study to report the disease burden of Chinese PD and Parkinsonism patients. It provides useful information to better understand their health status, and to raise the awareness of clinicians for providing better health care.
Zeylenone, a naturally occurring cyclohexene oxide, inhibits proliferation and induces apoptosis in cervical carcinoma cells via PI3K/AKT/mTOR and MAPK/ERK pathways
Authors: Zhang Leilei , Huo Xiaowei , Liao Yonghong , Yang Feifei , Gao Li , Cao Li 
Published: May 2017

AbstractThere is a strong rationale to therapeutically target the PI3K/Akt/mTOR and MAPK/ERK pathways in cervical carcinoma since they are highly deregulated in this disease. Previous study by our group have demonstrated that Zeylenone (Zey) exhibited strong suppressive activity on PI3K/AKT/mTOR and MAPK/ERK signaling, providing a foundation to investigate its antitumor activity in cervical carcinoma. Herein, the present study aimed to investigate suppressive effect of Zey on HeLa and CaSki cells, and further explore the underlying mechanisms. Cells were treated with Zey for indicated time, followed by measuring its effects on cell viability, colony formation, cell cycle, cell apoptosis, and signal pathways. In vivo antitumor activity of Zey was then assessed with nude xenografts. We found that Zey substantially suppressed cell proliferation, induced cell cycle arrest, and increased cell apoptosis, accompanied by increased production of ROS, decreased mitochondrial membrane potential, activated caspase apoptotic cascade, and attenuated PI3K/Akt/mTOR and MAPK/ERK pathways. Additionally, in vivo experiments showed that Zey exerted good antitumor efficacy against HeLa bearing mice models via decreasing levels of p-PI3K and p-ERK. Collectively, these data clearly demonstrated the antitumor activity of Zey in cervical carcinoma cells, which is most likely via the regulation of PI3K/Akt/mTOR and MAPK/ERK pathways.
Geometric explanation of the rich-club phenomenon in complex networks
Authors: Csigi Máté , Kőrösi Attila , Bíró József , Heszberger Zalán , Malkov Yury , Gulyás András 
Published: May 2017

AbstractThe rich club organization (the presence of highly connected hub core in a network) influences many structural and functional characteristics of networks including topology, the efficiency of paths and distribution of load. Despite its major role, the literature contains only a very limited set of models capable of generating networks with realistic rich club structure. One possible reason is that the rich club organization is a divisive property among complex networks which exhibit great diversity, in contrast to other metrics (e.g. diameter, clustering or degree distribution) which seem to behave very similarly across many networks. Here we propose a simple yet powerful geometry-based growing model which can generate realistic complex networks with high rich club diversity by controlling a single geometric parameter. The growing model is validated against the Internet, protein-protein interaction, airport and power grid networks.
In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications
Authors: Keriquel Virginie , Oliveira Hugo , Rémy Murielle , Ziane Sophia , Delmond Samantha , Rousseau Benoit , Rey Sylvie , Catros Sylvain , Amédée Joelle , Guillemot Fabien , Fricain Jean-Christophe 
Published: May 2017

AbstractBioprinting has emerged as a novel technological approach with the potential to address unsolved questions in the field of tissue engineering. We have recently shown that Laser Assisted Bioprinting (LAB), due to its unprecedented cell printing resolution and precision, is an attractive tool for the in situ printing of a bone substitute. Here, we show that LAB can be used for the in situ printing of mesenchymal stromal cells, associated with collagen and nano-hydroxyapatite, in order to favor bone regeneration, in a calvaria defect model in mice. Also, by testing different cell printing geometries, we show that different cellular arrangements impact on bone tissue regeneration. This work opens new avenues on the development of novel strategies, using in situ bioprinting, for the building of tissues, from the ground up.
Biomechanical analysis of prey capture in the carnivorous Southern bladderwort (Utricularia australis)
Authors: Poppinga Simon , Daber Lars Erik , Westermeier Anna Sofia , Kruppert Sebastian , Horstmann Martin , Tollrian Ralph , Speck Thomas 
Published: May 2017

AbstractWe recorded capture events (CEs) of the daphniid Ceriodaphnia dubia by the carnivorous Southern bladderwort with suction traps (Utricularia australis). Independent to orientation and behavior during trap triggering, the animals were successfully captured within 9 ms on average and sucked in with velocities of up to 4 m/s and accelerations of up to 2800 g. Phases of very high acceleration during onsets of suction were immediately followed by phases of similarly high deceleration (max.: −1900 g) inside the bladders, leading to immobilization of the prey which then dies. We found that traps perform a ‘forward strike’ during suction and that almost completely air-filled traps are still able to perform suction. The trigger hairs on the trapdoors can undergo strong bending deformation, which we interpret to be a safety feature to prevent fracture. Our results highlight the elaborate nature of the Utricularia suction traps which are functionally resilient and leave prey animals virtually no chance to escape.
Identification of Long Noncoding RNAs Deregulated in Papillary Thyroid Cancer and Correlated with BRAFV600E Mutation by Bioinformatics Integrative Analysis
Authors: Goedert Lucas , Plaça Jessica Rodrigues , Fuziwara Cesar Seigi , Machado Maiaro Cabral Rosa , Plaça Desirée Rodrigues , Almeida Palloma Porto , Sanches Talita Perez , Santos Jair Figueredo dos , Corveloni Amanda Cristina , Pereira Illy Enne Gomes , de Castro Marcela Motta , Kimura Edna Teruko , Silva Wilson Araújo , Espreafico Enilza Maria 
Published: May 2017

AbstractPapillary Thyroid Cancer (PTC) is an endocrine malignancy in which BRAFV600E oncogenic mutation induces the most aggressive phenotype. In this way, considering that lncRNAs are arising as key players in oncogenesis, it is of high interest the identification of BRAFV600E-associated long noncoding RNAs, which can provide possible candidates for secondary mechanisms of BRAF-induced malignancy in PTC. In this study, we identified differentially expressed lncRNAs correlated with BRAFV600E in PTC and, also, extended the cohort of paired normal and PTC samples to more accurately identify differentially expressed lncRNAs between these conditions. Indirectly validated targets of the differentially expressed lncRNAs in PTC compared to matched normal samples demonstrated an involvement in surface receptors responsible for signal transduction and cell adhesion, as well as, regulation of cell death, proliferation and apoptosis. Targets of BRAFV600E-correlated lncRNAs are mainly involved in calcium signaling pathway, ECM-receptor interaction and MAPK pathway. In summary, our study provides candidate lncRNAs that can be either used for future studies related to diagnosis/prognosis or as targets for PTC management.
Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata
Authors: Kaplanoglu Emine , Chapman Patrick , Scott Ian M. , Donly Cam 
Published: May 2017

AbstractCurrent control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle,Leptinotarsa decemlineata(Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15,CYP4Q3andCYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1andUGT2). Further, we demonstrated that silencing ofCYP4Q3and UGT2significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.
Acoustoelectric Current in Graphene Nanoribbons
Authors: Poole T. , Nash G. R. 
Published: May 2017

AbstractSurface acoustic waves (SAWs) propagating on piezoelectric substrates offer a convenient, contactless approach to probing the electronic properties of low-dimensional charge carrier systems such as graphene nanoribbons (GNRs). SAWs can also be used to transport and manipulate charge for applications such as metrology and quantum information. In this work, we investigate the acoustoelectric effect in GNRs, and show that an acoustoelectric current can be generated in GNRs with physical widths as small as 200 nm at room temperature. The positive current in the direction of the SAWs, which corresponds to the transportation of holes, exhibits a linear dependence on SAW intensity and frequency. This is consistent with the description of the interaction between the charge carriers in the GNRs and the piezoelectric fields associated with the SAWs being described by a relatively simple classical relaxation model. Somewhat counter-intuitively, as the GNR width is decreased, the measured acoustoelectric current increases. This is thought to be caused by an increase of the carrier mobility due to increased doping arising from damage to the GNR edges.
Layered social influence promotes multiculturality in the Axelrod model
Authors: Battiston Federico , Nicosia Vincenzo , Latora Vito , Miguel Maxi San 
Published: May 2017

AbstractDespite the presence of increasing pressure towards globalisation, the coexistence of different cultures is a distinctive feature of human societies. However, how multiculturality can emerge in a population of individuals inclined to imitation, and how it remains stable under cultural drift, i.e. the spontaneous mutation of traits in the population, still needs to be understood. To solve such a problem, we propose here a microscopic model of culture dissemination which takes into account that, in real social systems, the interactions are organised in various layers corresponding to different interests or topics. We show that the addition of multiplexity in the modeling of our society generates qualitatively novel dynamical behavior, producing a new stable regime of cultural diversity. This finding suggests that the layered organisation of social influence typical of modern societies is the key ingredient to explain why and how multiculturality emerges and thrives in our world.
Consonant Acquisition in Young Cochlear Implant Recipients and Their Typically Developing Peers
Authors: Iyer Suneeti Nathani , Jung Jongmin , Ertmer David J. 
Published: May 2017

Purpose
Consonant acquisition was examined in 13 young cochlear implant (CI) recipients and 11 typically developing (TD) children.

Method
A longitudinal research design was implemented to determine the rate and nature of consonant acquisition during the first 2 years of robust hearing experience. Twenty-minute adult–child (typically a parent) interactions were video and audio recorded at 3-month intervals following implantation until 24 months of robust hearing experience was achieved. TD children were similarly recorded between 6 and 24 months of age. Consonants that were produced twice within a 50-utterance sample were considered “established” within a child's consonant inventory.

Results
Although the groups showed similar trajectories, the CI group produced larger consonant inventories than the TD group at each interval except for 21 and 24 months. A majority of children with CIs also showed more rapid acquisition of consonants and more diverse consonant inventories than TD children.

Conclusions
These results suggest that early auditory deprivation does not significantly affect consonant acquisition for most CI recipients. Tracking early consonant development appears to be a useful way to assess the effectiveness of cochlear implantation in young recipients.
V‐band dual‐polarised dielectric resonator antenna with bondwire feeding structures
Authors: Lin Ta‐Yeh , Chiu Tsenchieh , Chang Da‐Chiang 
Published: May 2017

In this Letter, a V‐band chip‐level dual‐polarised dielectric resonator antenna implemented by using bondwires and silicon‐based integrated passive device technology is proposed. The square‐shaped resonator is fed by two bondwire coupling structures which excite two degenerate modes orthogonal to each other. The resonance of bondwire itself is also found to enhance the antenna bandwidth to cover the 60 GHz band. Reasonable agreement between the simulation and the measurement is obtained. The measured antenna bandwidth is from 52.8 to 65 GHz. The measured isolation is better than 20 dB at frequencies of interest. The measured antenna gain is 4.5 dBi at 60 GHz. The proposed design can provide further applications for circularly polarised systems.
Complex Skull Base Reconstructions in Kadish D Esthesioneuroblastoma: Case Report
Authors: Palejwala Sheri , Sharma Saurabh , Le Christopher , Chang Eugene , Erman Audrey , Lemole G. 
Published: May 2017

Introduction Advanced Kadish stage esthesioneuroblastoma requires more extensive resections and aggressive adjuvant therapy to obtain adequate disease-free control, which can lead to higher complication rates. We describe the case of a patient with Kadish D esthesioneuroblastoma who underwent multiple surgeries for infectious, neurologic, and wound complications, highlighting potential preventative and salvage techniques.
Case Presentation A 61-year-old man who presented with a large left-sided esthesioneuroblastoma, extending into the orbit, frontal lobe, and parapharyngeal nodes. He underwent margin-free endoscopic-assisted craniofacial resection with adjuvant craniofacial and cervical radiotherapy and concomitant chemotherapy. He then returned with breakdown of his skull base reconstruction and subsequent frontal infections and ultimately received 10 surgical procedures with surgeries for infection-related issues including craniectomy and abscess evacuation. He also had surgeries for skull base reconstruction and CSF leak, repaired with vascularized and free autologous grafts and flaps, synthetic tissues, and CSF diversion.
Discussion Extensive, high Kadish stage tumors necessitate radical surgical resection, radiation, and chemotherapy, which can lead to complications. Ultimately, there are several options available to surgeons, and although precautions should be taken whenever possible, risk of wound breakdown, leak, or infection should not preclude radical surgical resection and aggressive adjuvant therapies in the treatment of esthesioneuroblastoma.
Relook endoscopy predicts the development of esophageal and antropyloric stenosis better than immediate endoscopy in patients with caustic ingestion
Authors: Kochhar Rakesh , Ashat Munish , Reddy Yalaka , Dhaka Narender , Manrai Manish , Sinha Saroj , Dutta Usha , Yadav Thakur , Gupta Vikas 
Published: May 2017

Abstract
Background and aims Early ( < 24 hours) esophagogastroduodenoscopy (EGD) is used to prognosticate mucosal injury after caustic ingestion. We aimed to compare differences in endoscopic grading on EGDs performed on day 5 and day 1 and to assess the impact of relook endoscopy findings on the development of esophageal and/or antropyloric cicatrization.
Patients and methods Consecutive patients admitted within 24 hours of caustic ingestion between 2009 and 2014 underwent EGD and had their mucosal changes graded. Injuries of grade ≤ 2a were classified as mild and ≥ 2b were classified as severe. Patients were followed up for the development of cicatrization and managed per protocol. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and likelihood ratios (LRs) were calculated to compare day 1 and day 5 EGD findings.
Results A total of 62 patients (35 men; mean age 33 ± 15) underwent both day 1 and day 5 EGDs. Antropyloric stenosis developed in 16 patients, esophageal strictures in nine, and four had both esophageal and antropyloric strictures. Compared with day 1 EGD, endoscopic grading of severe injury on day 5 had higher specificity (83 % vs. 65 %), higher PPV (60 % vs. 41 %), and higher positive LR (5.65 vs. 2.66) for predicting the development of esophageal stricture. Similarly, day 5 endoscopic grading had higher specificity (95 % vs. 61 %), higher PPV (88 % vs. 54 %), and higher positive LR (16 vs. 2.5) for predicting the development of antropyloric stenosis.
Conclusion Endoscopic assessment on day 5 is a better predictor of esophageal and gastric cicatrization than day 1 EGD, which significantly overestimates the grade of injury.