bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: Correlation of the expressions of IGF1R‐RACK1‐STAT3 and Bcl‐xl in nasopharyngeal carcinoma with the clinicopathological features and prognosis of nasopharyngeal carcinoma
Authors: Wang Shan , Shen Min , Wen Xin , Han Xin‐Rui , Wang Yong‐Jian , Fan Shao‐Hua , Zhuang Juan , Zhang Zi‐Feng , Shan Qun , Li Meng‐Qiu , Hu Bin , Sun Chun‐Hui , Ge Xia , Lei Qiu‐Mei , Wu Dong‐Mei , Lu Jun , Zheng Yuan‐Lin 
Published: August 2017

AbstractThe aim of this study was to investigate the correlation of expression of IGF1R‐RACK1‐STAT3 and Bcl‐xl in nasopharyngeal carcinoma (NPC) with the clinicopathological features and the prognosis of NPC. Our study selected 215 NPC tissues and 178 chronic nasopharyngitis tissues (control group). Positive expression rates of IGF1R, RACK1, STAT3, and Bcl‐xl were tested by immunohistochemical method, and expression of IGF1R, RACK1, STAT3, Bcl‐xl, Bcl‐2, and Bax by western blotting. Correlation of IGF1R, RACK1, STAT3, and Bcl‐xl with the clinicopathological features of NPC was analyzed. The correlation among those four expression was analyzed by Spearman. The survival of NPC and independent factors of prognosis were tested by Kaplan‐Meier and COX proportional hazards model respectively. The NPC group had higher positive expression rates of IGF1R, RACK1, STAT3, and Bcl‐xl, and elevated expression of IGF1R, RACK1, STAT3, Bcl‐xl, Bcl‐2, and Bax. The lymph node metastasis (LNM) group had higher positive expression rates of IGF1R and RACK1 when compared with the non‐LNM group. Patients with stage III and IV had higher positive expression rates of IGF1R, RACK1, STAT3, and Bcl‐xl. There was positive correlation between expression of IGF1R and RACK1, STAT3. Such correlation was found between RACK1 and STAT3. Patients with negative expression of IGF1R, RACK1, STAT3, and Bcl‐xl had higher survival rates. The risky factors of poor prognosis of NPC were positive expression of IGF1R, RACK1, STAT3 and Bcl‐xl, and LNM. IGF1R‐RACK1‐STAT3 and Bcl‐xl expression correlated with the clinicopathological features and poor prognosis of NPC.
Genistein represses PEPCK‐C expression in an insulin‐independent manner in HepG2 cells and in alloxan‐induced diabetic mice
Authors: Dkhar Barilin , Khongsti Kitboklang , Thabah Daiahun , Syiem Donkupar , Satyamoorthy Kapaettu , Das Bidyadhar 
Published: August 2017

AbstractGenistein has been reported to exert beneficial effects on type 2 diabetes mellitus (T2DM); however, the underlying molecular mechanisms involved therein have not been clearly elucidated. To address this question, the effect of genistein on the expression of phosphoenolpyruvate carboxykinase (PEPCK), and glucose production in HepG2 cells and in alloxan‐induced diabetic mice was investigated. HepG2 cells were exposed to different concentration of genistein in presence or absence of modulators, and the expression of cytosolic PEPCK (PEPCK‐C) and the signaling pathways was studied. Further, the biological relevance of the in vitro study was tested in alloxan‐induced diabetic mice. Genistein lowered PEPCK‐C expression and glucose production in HepG2 cells accompanied with increased in phosphorylation states of AMPK, MEK½, ERK½, and CRTC2. Treatment with the AMPK inhibitor (compound C) enhanced genistein‐induced MEK½ and ERK½ activity indicating a potential cross‐talk between the two signaling pathways. In vivo, genistein also reduced fasting glucose levels accompanied with reduced PEPCK‐C expression and increased in AMPK and ERK½ phosphorylation states in the liver of genistein‐treated alloxan‐induced diabetic mice. Genistein fulfills the criteria of a suitable anti‐diabetic agent by reducing glucose production and inhibiting PEPCK‐C expression in HepG2 cells and also in alloxan‐induced diabetic mice. These results indicate that genistein is an effective candidate for preventing T2DM through the modulation of AMPK‐CRTC2 and MEK/ERK signaling pathways, which may allow a novel approach to modulate dysfunction in hepatic gluconeogenesis in T2DM.
Introduction of hsa‐miR‐103a and hsa‐miR‐1827 and hsa‐miR‐137 as new regulators of Wnt signaling pathway and their relation to colorectal carcinoma
Authors: Fasihi Ali , M. Soltani Bahram , Atashi Amir , Nasiri Shirzad 
Published: August 2017

AbstractWnt signaling is hyper‐activated in most of human cancers including colorectal carcinoma (CRC). Therefore, the introduction of new regulators for Wnt pathway possesses promising diagnostic and therapeutic applications in cancer medicine. Bioinformatics analysis introduced hsa‐miR‐103a, hsa‐miR‐1827, and hsa‐miR‐137 as potential regulators of Wnt signaling pathway. Here, we intended to examine the effect of these human miRNAs on Wnt signaling pathway components, on the cell cycle progression in CRC originated cell lines and their expression in CRC tissues. RT‐qPCR results indicated upregulation of hsa‐miR‐103a, hsa‐miR‐1827, and downregulation of hsa‐miR‐137 in CRC tissues. Overexpression of hsa‐miR‐103a and hsa‐miR‐1827 in SW480 cells resulted in elevated Wnt activity, detected by both Top/Flash assay and RT‐qPCR analysis. Inhibition of Wnt signaling by using PNU‐74654 or IWP‐2 small molecules suggested that these miRNAs exerts their effect at the β‐catenin degradation complex level. Then, RT‐qPCR, dual luciferase assay, and western blotting analysis indicated that APC and APC2 transcripts were targeted by hsa‐miR‐103a, hsa‐miR‐1827 while, Wnt3a and β‐catenin genes were upregulated. However, hsa‐miR‐137 downregulated Wnt3a and β‐catenin genes. Further, hsa‐miR‐103a and hsa‐miR‐1827 overexpression resulted in cell cycle progression and reduced apoptotic rate in SW480 cells, unlike hsa‐miR‐137 overexpression which resulted in cell cycle suppression, detected by flowcytometry and Anexin analysis. Overall, our data introduced hsa‐miR‐103a, hsa‐miR‐1827 as onco‐miRNAs and hsa‐miR‐137 as tumor suppressor which exert their effect through regulation of Wnt signaling pathway in CRC and introduced them as potential target for therapy.
Autophagy induction plays a protective role against hypoxic stress in human dental pulp cells
Authors: Park Sam‐Young , Sun Eun‐Gene , Lee Yeonju , Kim Min‐Seok , Kim Jae‐Hyung , Kim Won‐Jae , Jung Ji‐Yeon 
Published: August 2017

AbstractHuman dental pulp exposed to hypoxic conditions induces cell death accompanied by autophagy. However, the role of hypoxia‐induced autophagy in human dental pulp cells (HDPCs) is unclear. The present study aimed to investigate the role of autophagy in hypoxia‐induced apoptosis of HDPCs. Cobalt chloride (CoCl2) treated HDPCs, to mimic hypoxic conditions, decreased cell viability. Also, apoptosis‐related signal molecules, cleaved caspase‐3 and PARP levels, were enhanced in CoCl2‐treated HDPCs. HDPCs exposed to CoCl2 also promoted autophagy, showing upregulated p62 and microtubule‐associated protein 1 light chain 3 (LC3)‐II levels, typical autophagic markers, and increased acidic autophagolysosomal vacuoles. Autophagy inhibition by 3 methyladenine (3MA) or RNA interference of LC3B resulted in increased levels of cleaved PARP and caspase‐3, and the release of cytochrome c from mitochondria into cytosol in the CoCl2‐treated HDPCs. However, autophagy activation by rapamycin enhanced the p62 and LC3‐II levels, whereas it reduced PARP and caspase‐3 cleavage induced by CoCl2. These results revealed that CoCl2‐activated autophagy showed survival effects against CoCl2‐induced apoptosis in the HDPCs. CoCl2 upregulated HIF‐1α and decreased the phosphorylation of mTOR/p70S6K. HIF‐1α inhibitor, YC‐1 decreased p62 and LC3‐II levels, whereas it augmented PARP and caspase‐3 cleavage in response to CoCl2. Also, YC‐1 enhanced the phosphorylation of mTOR and p70S6K suppressed by CoCl2, demonstrating that CoCl2‐induced autophagy via mTOR/p70S6K is mediated by HIF‐1α. Taken together, these finding suggest that CoCl2‐induced autophagy mediated by the mTOR/p70S6K pathway plays a protective role against hypoxic stress in HDPCs.
Is there a real role of postoperative antibiotic administration for mildmoderate acute cholecystitis? A prospective randomized controlled trial
Authors: Kim Eun Young , Yoon Young Chul , Choi Ho Joong , Kim Kee Hwan , Park Jung Hyun , Hong Tae Ho 
Published: August 2017

AbstractBackgroundIn cases of acute cholecystitis (AC), postoperative antibiotic prophylaxis is generally used for the purpose of preventing subsequent infections. However, there is still no standardized guideline regarding antibiotic administration after cholecystectomy.MethodsA total of 200 patients at five participating hospitals who were admitted for cholecystectomy to treat grade I or II AC were enrolled and randomly allocated to a group given a placebo (group A) or a group given postoperative antibiotics (group B). Surgical outcomes and incidence of postoperative infectious morbidities were reviewed.ResultsA total of 188 patients (95 patients in group A and 93 patients in group B) were finally analyzed. The incidence rate of infectious complications (seven cases, 7.4%, in group A and eight cases, 8.6%, in group B, P = 0.794) and overall non‐infectious complications (seven cases, 7.4%, in group A and six cases, 6.5%, in group B, P = 1.000) showed no significant difference between the two groups.ConclusionsAbsence of postoperative antibiotic administration did not lead to an increase in postoperative infections in cases of mild to moderate AC. Avoidance of unnecessary antibiotic use will reduce the adverse effects of antibiotics and also allow for a tailored treatment strategy according to the severity of cholecystitis.
Performance of MRI for suspected appendicitis in pediatric patients and negative appendectomy rate: A systematic review and meta‐analysis
Authors: Kim Jeong Rye , Suh Chong Hyun , Yoon Hee Mang , Jung Ah Young , Lee Jin Seong , Kim Jung Heon , Lee Jeong‐Yong , Cho Young Ah 
Published: August 2017

PurposeTo evaluate in pediatric patients the diagnostic performance of magnetic resonance imaging (MRI) for suspected appendicitis and its negative appendectomy rate.Materials and MethodsThe study involved diagnostic accuracy studies that used MRI for pediatric patients with suspected appendicitis. Various field strengths and sequences were used in each included study. The diagnostic accuracy of MRI in pediatric patients with suspected appendicitis and negative appendectomy rate were assessed. A search of MEDLINE and EMBASE databases was performed until January 10, 2017. Two reviewers assessed the methodological quality of the articles using the Quality Assessment of Diagnostic Accuracy Studies‐2 (QUADAS‐2) tool. Pooled sensitivity and specificity for appendicitis diagnosis were calculated using hierarchical logistic regression modeling. Meta‐regression was performed to explore factors affecting study heterogeneity. The pooled negative appendectomy rate was analyzed.ResultsThirteen original articles with 1946 patients were included. The summary sensitivity and specificity were 97% (95% confidence interval [CI], 95–98%) and 97% (95% CI, 93–98%), respectively; the area under the hierarchical summary receiver operating characteristic (HSROC) curve was 0.98 (95% CI, 0.97–0.99). In meta‐regression, use of diffusion‐weighted imaging (P < 0.01) and age (P < 0.01) affected heterogeneity, but there was no difference according to intravenous contrast agent use (P = 0.95). The pooled negative appendectomy rate of MRI was 4.5% (95% CI, 1.9–7.1%).ConclusionMRI shows excellent diagnostic performance for appendicitis in pediatric patients regardless of the use of intravenous contrast media. Therefore, nonenhanced‐only MRI protocols might be appropriate for pediatric patients with suspected appendicitis.Level of Evidence: 3Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2018;47:767–778.
Impact of lignocellulosic and hemicellulosic biochar on soil moisture in low clay soils
Authors: Licht Jeff , Smith Nick , Mitchell Perry , Shields Frank 
Published: August 2017

AbstractInvestigation of post‐amendment biochar impact on low clay soil moisture provides agriculture professionals with much needed data. While laboratory testing is available, we propose inexpensive containers, tools and measuring devices to enable agriculture professionals to directly assess biochar impact on gravimetric water content, shrinkage, and release at point of soil rupture. Sandy loam, silty loam and loamy sand soils are amended (10% ) with lignocellulosic (oak) and hemicellulosic (cardboard) biochars in cup, plug and roll experiments. Cups with oak and cardboard biochar addition produced 76.32% and 75.72% H2O retention respectively, compared to 67.75% (67.75 g H2O 100 g−1 H2O) for controls. Cardboard and oak biochar limited diametric shrinkage to 2.95% (1.29 mm) and 3.75% (1.65 mm) respectively; controls shrunk 6.96% (3.06 mm). Oak and cardboard biochar limited depth shrinkage to 2.95% (0.38 mm) and 2.99% (0.38 mm) respectively; control depth shrinkage is 3.64% (0.47 mm). In roll tests, cardboard and oak biochar treatment yielded 28.07% (1.37 g H2O), and 26.69% (1.3 g H2O) moisture at rupture, respectively, compared with 11.98% (0.58 g) for controls. Significant (p ≤ 0.001) differences in moisture retention, shrinkage and available moisture at rupture confirm biochar contributions to improved moisture performance. Physico‐chemical analyses complemented experimental findings. We find study methods suit the needs of agricultural professionals to measure moisture while working with biochar to amend soils.
The cost of being clean: A cost analysis of nasopharyngoscope reprocessing techniques
Authors: Sowerby Leigh J. , Rudmik Luke 
Published: August 2017

ObjectiveNasopharyngoscopes are an essential instrument to otolaryngologists; reprocessing them in a high‐value manner is paramount. Although several different techniques for reprocessing exist, all methods yield similar effectiveness. Given equivalent effectiveness outcomes, a cost analysis of four nasopharyngoscope reprocessing techniques was performed.Study DesignCost‐minimization analysis.MethodsFour techniques were evaluated: 1) an automated reprocessor using peracetic acid (Steris System 1; Steris Canada Inc., Mississauga, Canada), 2) an automated reprocessor using ortho‐phthalaldehyde (OPA) (Cidex OPA; Advanced Sterilization Products, Johnson and Johnson Inc., Markham, Canada), 3) a manually performed accelerated hydrogen peroxide bath (Revital‐Ox; Steris Canada Inc.), and 4) a chlorine dioxide wipe (Tristel Trio Wipes System; Tristel plc, Cambridgeshire, U.K.). The costing perspective was a third‐party payer that was adjusted to 2014 Canadian dollars. The base‐case scenario used an annual volume of 4,153 reprocessing events in a tertiary care setting, and a scenario analysis assessed the impact of volume and capital expense.ResultsThe cost per reprocessing event for the Steris (Steris Canada Inc.) automated endoscope reprocessing, Cidex OPA (Advanced Sterilization Products), Revital‐Ox (Steris Canada Inc.), and Tristel Trio Wipes (Tristel plc) were $20.58, $14.20, $9.57, and $13.14, respectively. Scenario analysis demonstrated the Tristel Trio Wipes System (Tristel plc) was the least expensive method in practices with low reprocessing volumes (a threshold of less than 6 events per day, or 22 per week), whereas the Revital‐Ox (Steris Canada Inc.) system was least expensive at higher volumes and became substantially more so as volumes increased.ConclusionA manual accelerated hydrogen peroxide bath offers the least costly approach to nasopharyngoscope reprocessing. The convenience and portability of the Tristel Trio (Tristel plc) system may be a good alternative for low reprocessing volumes, or when rapid turnaround is necessary.Level of EvidenceNA. Laryngoscope, 128:64–71, 2018
Predictors of complicated airway foreign body extraction
Authors: Sjogren Phayvanh P. , Mills Tyler J. , Pollak Adrianna D. , Muntz Harlan R. , Meier Jeremy D. , Grimmer J. Fredrik 
Published: August 2017

ObjectivesTo evaluate outcomes of foreign body aspiration (FBA) and to investigate surgeon and hospital volume as risk factors for a complicated course.Study DesignRetrospective case series.MethodsChildren with FBA in a multihospital network were identified from January 2005 to September 2015. Demographic information, surgeon, and hospital location were reviewed. Mean operative time and hospital length of stay were recorded. Cases requiring intensive care unit admission, hospital stay greater than 24 hours, need for more than one bronchoscopy, operative time greater than 1 hour, or death were considered “complicated.”ResultsA total of 450 cases of airway foreign body extraction were performed. Patient ages ranged from 0.6 to 18.8 years, with a median age of 1.9 years. Bronchoscopy with foreign body extraction was performed by 55 different surgeons at 11 different facilities. There were one to 24 surgeons for each facility, with an average number of 5.4 surgeons per facility. A total of 88 (19.6%) cases were considered complicated, including five (1.1%) deaths. Increased rates of complications were seen with unwitnessed aspiration (P = 0.008) and hyperlucency (P < 0.001) or infiltrates (P = 0.001) on chest radiographs. No significant association was found between surgeon type or facility as related to a complicated case.ConclusionsUnwitnessed aspiration events and abnormalities on chest radiograph may be associated with a more complicated course in children with FBA. This multihospital study identified a low number of procedures by many surgeons; however, surgeon and hospital volume did not significantly correlate with higher complication rates.Level of Evidence4. Laryngoscope, 128:490–495, 2018
Factors associated with patient no‐show rates in an academic otolaryngology practice
Authors: Fiorillo Caitlin E. , Hughes Allyson L. , I‐Chen Chen , Westgate Philip M. , Gal Thomas J. , Bush Matthew L. , Comer Brett T. 
Published: August 2017

Objectives/HypothesisFactors affecting access to healthcare is an expanding area of research. This study seeks to identify factors associated with no‐show rates in an academic otolaryngology practice to improve clinical efficiency and patient access to care.Study DesignRetrospective review.MethodsA retrospective review of scheduled clinical appointments from February 1, 2015 to January 30, 2016 at a single academic otolaryngology department was performed. Statistical analysis was completed to examine the association of no‐show rates with the following: otolaryngology subspecialty, clinic location (e.g., main campus vs. satellite), patient demographic factors, attending seniority, temporal factors, insurance types, rurality, and visit type.ResultsThere was an overall no‐show rate of 20% for 22,759 scheduled clinic visits. Satellite clinics had the highest no‐show rates at 25% (P < .001). New patient visits had the highest no‐show rate at 24% (P < .001). Among subspecialties, facial plastic surgery had the lowest no‐show rate (12.6%), whereas Pediatrics had the highest (23%) (P < .001). No significant association between gender and no‐show rates was observed (P = .29), but patients over 60 years old had the lowest no‐show rate (12.7%, P < .0001). Patients with Medicaid (28%), Medicare (15.3%), and commercial insurance (12.9%) had significantly different overall no‐show rates (P < .0001).ConclusionsIncreased clinic no‐show rates are associated with satellite clinics, new patient visits, younger age, and insurance type. No‐show rates varied among subspecialties. Further investigation is warranted to assess barriers to appointment compliance and to develop interventions to improve access to care.Level of Evidence4. Laryngoscope, 128:626–631, 2018
Morbidity and mortality in children undergoing bronchoscopy for foreign body removal
Authors: Roberts Christopher A. , Carr Michele M. 
Published: August 2017

Objectives/HypothesisAnalyze morbidity and mortality among children undergoing bronchoscopy for foreign body removal.Study DesignMulticenter retrospective review using the American College of Surgeons Pediatric National Surgical Quality Improvement Program from 2014 and 2015.MethodsPatients were identified using Current Procedural Terminology code 31635. Demographics, time to surgery, operative times, hospitalization time, and complications were collected. Multivariate logistic regression was used to identify predictive factors for major adverse events.ResultsThree hundred thirty‐four patients were included (mean age 3.7 years, 59.0% male). Preoperative sepsis syndrome was present in 5.7% of patients and 8.1% had asthma. Of the patients, 5.1% percent of patients had a tracheostomy. Bronchoscopy was performed by an otolaryngologist (65.4%) or a pediatric surgeon (33.1%). Mean operative time was 27.4 minutes, whereas mean total operating room time was 54.6 minutes. Airway foreign bodies were located in 269 patients (80.5%), with 62.5% being located in the mainstem bronchus. Operative time was longer when foreign bodies were in the mainstem bronchus or distal to it. Mean time to surgery from admission was 0 days, and mean duration of hospitalization was 1 day. One patient (0.3%) required reoperation for respiratory reasons, and three (0.9%) required readmission for related reasons. No patients remained hospitalized at 30 days. Two (0.6%) had a postoperative pneumonia, and two (0.6%) required reintubation. One patient death (0.3%) occurred within 2 weeks of bronchoscopy. No significant differences were identified in operative time, time to surgery, or hospitalization time based on age, gender, presence of a tracheostomy, or surgical specialty.ConclusionsBronchoscopy for identification and removal of airway foreign bodies had minimal morbidity in this group.Level of Evidence2b. Laryngoscope, 128:1226–1229, 2018
Partial inferior turbinectomy in rhinoseptoplasty has no effect in quality‐of‐life outcomes: A randomized clinical trial
Authors: de Moura Bianca H. , Migliavacca Raphaella O. , Lima Rafaela K. , Dolci José E. L. , Becker Martina , Feijó Cássia , Brauwers Elisa , Lavinsky‐Wolff Michelle 
Published: August 2017

ObjectiveEvaluate the impact of endoscopic partial inferior turbinectomy (EPIT) associated with primary rhinoseptoplasty on quality‐of‐life outcomes (QOL), complications, and surgical duration.Study DesignRandomized clinical trial.MethodsIndividuals with nasal obstruction aged ≥ 16 years who were candidates for functional and aesthetics primary rhinoseptoplasty were evaluated from March 2014 through May 2015. Eligible participants were randomly allocated to rhinoseptoplasty with or without EPIT (excision of one‐third of the inferior turbinates).ResultsFifty patients were studied. Most were Caucasian and had moderate/severe allergic rhinitis symptoms. Mean age was 36 ( ± 14.5) years. Rhinoseptoplasty was associated with improvement in all QOL scores irrespective of turbinate intervention (P < 0.001). Analysis of covariance was conducted to control for potential confounders. There was no difference between the groups in absolute score changes for Nasal Obstruction Symptom Evaluation‐Portuguese (NOSE‐p) (−50.5 vs. −47.6; P  = 0.723), Rhinoplasty Outcome Evaluation (ROE) (47 vs. 44.8; P = 0.742), and all World Health Organization Quality of Life Scale‐Abbreviated (WHOQOL‐bref) score domains (P  > 0.05). There were no differences between the groups regarding presence of the complications. Surgical duration was higher in the EPIT group (212 minutes ± 7.8 vs. 159.1 ± 5.6; P ? 0.001).ConclusionsTurbinate reduction through EPIT during primary rhinoseptoplasty did not improve short‐term general and specific QOL outcomes. The use of EPIT increases surgical time considerably without improving QOL scores. There was no difference in postoperative incidence of complications, suggesting that EPIT is a safe technique.Level of Evidence1b. Laryngoscope, 128:57–63, 2018