bluuebunny/crossref_metadata_2025_split huggingface dataset:
Living with Pompe disease: results from a qualitative interview study with children and adolescents and their caregivers
Authors: Truninger Moritz Ilan , Werner Helene , Landolt Markus Andreas , Hahn Andreas , Hennermann Julia B. , Lagler Florian B. , Möslinger Dorothea , Pfrimmer Charlotte , Rohrbach Marianne , Huemer Martina 
Published: September 2024

Abstract
Background
Children and adolescents with Pompe disease (PD) face chronic and progressive myopathy requiring time-intensive enzyme replacement therapy (ERT). Little is known about their perspectives on the disease and its treatment. This study explored their perceptions of disease symptoms and functioning status, and more subjective feelings about the impacts on their lives as part of developing a disease-specific questionnaire.

Methods
Eleven pediatric patients aged 8–18 years and 26 caregivers from six children’s hospitals in Germany, Austria, and Switzerland underwent semi-structured interviews. Data were recorded, transcribed using MAXQDA software, and analyzed using qualitative content analysis. A system of meaningful categories was developed.

Results
Sixteen main categories were derived across four major thematic areas: perceptions of symptoms and limitations, experiences to do with the biopsychosocial impact of PD, treatment experiences, and general emotional well-being/burden. Participants demonstrated broad heterogeneity in symptom perceptions such as muscle weakness, breathing difficulties, pain, and fatigue. Emotional appraisals of limitations were not directly proportional to their severity, and even comparatively minor impairments were often experienced as highly frustrating, particularly for social reasons. The main psychosocial topics were social exclusion vs. inclusion and experiences to do with having a disease. The main finding regarding treatment was that switching ERT from hospital to home was widely viewed as a huge relief, reducing the impact on daily life and the burden of infusions. Emotional well-being ranged from not burdened to very happy in most children and adolescents, including the most severely affected.

Conclusion
This study provided qualitative insights into the perceptions and experiences of pediatric PD patients. Interestingly, biopsychosocial burden was not directly related to disease severity, and tailored psychosocial support could improve health-related quality of life. The present findings ensure the content validity of a novel questionnaire to be tested as a screening tool to identify patients in need of such support.
Focusing on CD8+ T-cell phenotypes: improving solid tumor therapy
Authors: Yao Zhouchi , Zeng Yayun , Liu Cheng , Jin Huimin , Wang Hong , Zhang Yue , Ding Chengming , Chen Guodong , Wu Daichao 
Published: September 2024

AbstractVigorous CD8+ T cells play a crucial role in recognizing tumor cells and combating solid tumors. How T cells efficiently recognize and target tumor antigens, and how they maintain the activity in the “rejection” of solid tumor microenvironment, are major concerns. Recent advances in understanding of the immunological trajectory and lifespan of CD8+ T cells have provided guidance for the design of more optimal anti-tumor immunotherapy regimens. Here, we review the newly discovered methods to enhance the function of CD8+ T cells against solid tumors, focusing on optimizing T cell receptor (TCR) expression, improving antigen recognition by engineered T cells, enhancing signal transduction of the TCR-CD3 complex, inducing the homing of polyclonal functional T cells to tumors, reversing T cell exhaustion under chronic antigen stimulation, and reprogramming the energy and metabolic pathways of T cells. We also discuss how to participate in the epigenetic changes of CD8+ T cells to regulate two key indicators of anti-tumor responses, namely effectiveness and persistence.
A rapid tick exposure test for monitoring acaricide resistance in Rhipicephalus sanguineus sensu lato ticks on dogs
Authors: Jongejan Frans , Berger Laura , Papadopoulos Elias , Reck José , Ferreira Priscila Teixeira , Scott Fabio Barbour , de Avelar Barbara Rauta , Guimarães Brena Gava , Correia Thais Ribeiro , Hulsebos Iris , Petersen Alita , Klafke Guilherme 
Published: September 2024

Abstract
Background
Brown dog ticks (Rhipicephalus sanguineus sensu lato) are vectors of pathogens adversely affecting the health of dogs in many regions of the world. The three-host life cycle of R. sanguineus s.l., with all stages feeding on dogs, can lead to an uncontrolled build-up of large tick populations if not controlled by acaricides. However, frequent tick control on dogs using acaricides has led to the emergence of resistance to permethrin and fipronil. Currently, the larval packet test (LPT) is the standard tick resistance test, which is laborious, requires laboratory facilities, and takes at least 6 weeks before larvae derived from engorged female ticks can be tested. Our novel approach is to expose semi-engorged adult ticks to acaricides immediately after removing them from dogs, obtaining results within 24 h.

Methods
Adult ticks from three laboratory colonies of R. sanguineus s.l. were tested in RaTexT®, a rapid tick exposure test in which ticks were confined to small compartments and exposed to an acaricide-impregnated, specially designed matrix. Resistance was confirmed by testing larvae derived from the same laboratory colonies using the LPT. RaTexT® was also used to determine the susceptibility of R. sanguineus acaricides in dog shelters.

Results
RaTexT® detected resistance to permethrin in adult R. sanguineus s.l. ticks from two Brazilian laboratory colonies compared to a susceptible laboratory strain originating in Greece. Resistance was confirmed by LPT testing of larvae from the same colonies with resistance factors between 2.2 and 3.1. All laboratory strains were susceptible to fipronil. A suspected case of fipronil resistance at a dog shelter in Caxias do Sul, Brazil, was resolved within 24 h by testing adult ticks in RaTexT® and could be attributed to improper treatment.

Conclusions
RaTexT® is a valuable tool for monitoring the development of resistance to synthetic pyrethroids or phenylpyrazoles in tick-infested dogs.

Graphical Abstract
Methotrexate-induced leukoencephalopathy presenting as acute-onset limb weakness in a child: a case report
Authors: Pathiraja Hashan , de Abrew Gayathri , de Silva Linushika , Fernando Sanjaya , Randeny Shobhavi , Mettananda Sachith 
Published: September 2024

Abstract
Background
Methotrexate is an essential medicine used to treat childhood malignancies including acute lymphoblastic leukemia. Neurotoxicity manifesting as leukoencephalopathy is an important adverse effect of methotrexate. Methotrexate-induced leukoencephalopathy classically demonstrates sub-acute-onset neurological manifestations that include learning disability, progressive dementia, drowsiness, seizures, ataxia, and hemiparesis. These are rare in children and are generally reported following intrathecal or intravenous use of methotrexate. In contrast, acute onset neurotoxicity with oral use of methotrexate is very rare. We report a 10-year-old boy presenting with acute onset limb weakness and neurological signs due to methotrexate-induced leukoencephalopathy following oral methotrexate.

Case presentation
A 10-year-old Sri Lankan boy presented with fever and headache for 5 days and difficulty in walking for 2 days. He was unable to stand unaided on admission, and his parents complained of repetitive, involuntary extension movements involving the right upper limb. He is a child diagnosed with acute lymphoblastic leukemia who was on treatment for a relapse with daily oral dexamethasone and mercaptopurine, weekly oral methotrexate and folinic acid, and once every two weeks intrathecal vincristine.
On examination, he had dystonic movements of the right upper limb and hypotonia and reduced muscle power (grade 3/5) of the left upper and lower limbs proximally and distally. The muscle power of the right side was grade 4 (out of 5). Tendon reflexes were diminished in all four limbs, and the plantar response was flexor bilaterally. The child had dysmetria and intension tremors on both sides.
T2-weighted magnetic resonance imaging of the brain revealed symmetrical high signal intensities with diffusion restriction involving bilateral putamen, subcortical areas, and deep white matter, suggesting treatment-related neurotoxicity due to methotrexate-induced leukoencephalopathy. Oral methotrexate was discontinued. He showed gradual improvement in limb weakness and other neurological signs following treatment with intravenous folinic acid, aminophylline, dexamethasone, and oral dextromethorphan.

Conclusion
This case report describes a patient with rapidly progressing methotrexate-induced leukoencephalopathy following oral methotrexate. It highlights that the risk of neurotoxicity persists even with the oral use of methotrexate; therefore, the prescribers should be vigilant of this uncommon side effect.
Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils
Authors: Pandolfo Emiliana , Durán-Wendt David , Martínez-Cuesta Ruben , Montoya Mónica , Carrera-Ruiz Laura , Vazquez-Arias David , Blanco-Romero Esther , Garrido-Sanz Daniel , Redondo-Nieto Miguel , Martin Marta , Rivilla Rafael 
Published: September 2024

AbstractA bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1 mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.
How control and eradication of BVDV at farm level influences the occurrence of calf diseases and antimicrobial usage during the first six months of calf rearing
Authors: Dobos Attila , Dobos Vilmos , Kiss István 
Published: September 2024

Abstract
Background
Bovine viral diarrhoea (BVD) is one of the major cattle diseases causing economic losses worldwide. Nowadays the disease manifests mainly as virus-induced immunosuppression and early embryonic death, impacting overall herd performance and contributing to increased antibiotic usage in calf rearing.

Methods
In our study we investigated the effect of rapid BVDV control measures on calf diseases and antimicrobial usage after weaning on a large industrial dairy farm. Persistently infected (PI) animals were identified and removed from the herd within a short period of time, and all susceptible animals were vaccinated against BVDV. Recorded herd parameters and AB usage were monitored retrospectively and compared with data collected after starting the BVD control program.

Results and discussion
The programme began in January 2023 with identifying and eliminating PI animals from the farm. Twenty-one PI animals were found by using RT-qPCR testing of blood sera out of the 1571 animals tested (1.33%). Subsequent testing (January and December 2023) identified further 28 PI animals amongst the 542 calves tested shortly after birth, and all were instantly removed from the farm. In parallel with the BVDV eradication measures, AB usage dropped by more than 50% compared to previous years. Calf mortality also decreased from 7.45 to 4.38% as the control program progressed. Correspondingly, both the number of respiratory and diarrhoea cases decreased dramatically on the farm while the eradication measures were in place.

Conclusion
Our study clearly demonstrated the positive effects of BVDV eradication on the improvement of calf health and importantly, a reduction of AB usage, contributing to the One Health perspective of farm animal production.
Effectiveness of interventions for informal caregivers of people with end-stage chronic illness: a systematic review
Authors: McGuigan K. , Laurente G. , Christie A. , Carswell C. , Moran C. , Yaqoob M. M. , Bolton S. , Mullan R. , Rej S. , Gilbert P. , McKeaveney C. , McVeigh C. , Tierney C. , Reid J. , Walsh I. , Forbes T. , Noble H. 
Published: September 2024

Abstract
Background
People living with advanced, non-malignant chronic conditions often have extensive and complex care needs. Informal or family caregivers often provide the care and support needed by those with advanced chronic conditions at home. These informal caregivers experience many challenges associated with their caring role, which can impact their own wellbeing. Whilst there is growing evidence around the impact on carers, guidance on support for informal caregivers of patients with advanced, non-malignant, chronic conditions is lacking, with little evidence available on effective psychosocial carer interventions. This systematic review explored existing interventions for caregivers of those with advanced, non-malignant, chronic illness, in order to assess the effectiveness of these interventions in improving psychosocial outcomes.

Methods
Electronic databases, Medline, CINAHL, EMBASE, and PsycINFO, were searched up to the end of March 2023. Studies meeting the inclusion criteria, focusing on interventions to improve psychosocial outcomes, such as depression, anxiety, quality of life, and caregiver burden, in this cohort of caregivers were included. Data were extracted regarding study setting, design, methods, intervention components, and outcomes. Risk of bias and quality assessment were conducted.

Results
A total of 5281 articles were screened, ultimately identifying 12 studies for inclusion, reported in 13 publications. A narrative synthesis revealed mixed results. Psychosocial interventions resulted in more significant improvements in psychosocial outcomes than psychoeducational or support interventions, with interventions for carer-patient dyads also reflecting more positive outcomes for caregivers. Evidence-based interventions, guided by an appropriate theoretical model, were reportedly more effective in improving caregiver outcomes. Differences in outcomes were related to intervention development, design, delivery, and outcome assessment.

Conclusions
This review, to our knowledge, is the first to explore the effectiveness of interventions in improving psychosocial outcomes for caregivers of those with advanced, non-malignant, chronic conditions. The review highlights the need for more robust, sufficiently powered, high-quality trials of evidence-based interventions for caregivers of people with advanced chronic illness. Optimal intervention duration and frequency of sessions are unclear and need further exploration.
Experiences and perceptions of patients with cancer receiving home-based chemotherapy: a qualitative systematic review protocol
Authors: Witwaranukool Porawan , Phonyiam Ratchanok , Wu Yanni , Kynoch kathryn 
Published: September 2024

Abstract
Background
Home-based chemotherapy (HBC) has emerged as a standard option for treating various types of cancer, primarily to decrease the waiting time for treatment. As HBC gains more recognition, ongoing research is delving into the experiences of patients with cancer who receive chemotherapy in a home setting or chemotherapy closer to home. Understanding these experiences is vital for the use of chemotherapy delivery outside the traditional hospital environments. This review aims to synthesize and critically appraise qualitative studies that investigate the experience and perspectives of patients with cancer who received parenteral chemotherapy administration in home settings. Findings will be used to develop evidence-based policies to support home-based care models.

Methods
This review will follow JBI methods for systematic reviews of qualitative evidence. The databases for searching will include MEDLINE (PubMed), CINAHL (EBSCOhost), PsycINFO (EBSCOhost), ProQuest Health and Medical Collection, two Chinese databases, CNKI and Wanfang, and one Thai database, ThaiJO. Studies published in English, Chinese, and Thai will be considered for inclusion. Two reviewers will independently undertake study selection, data extraction, and critical appraisal of the methodological quality of studies. The synthesized findings will be assessed using the ConQual approach.

Discussion
The synthesis of qualitative studies on this topic will provide insights into the nuanced and varied experiences of patients receiving chemotherapy within the comfort of their homes. The review will also provide evidence-based recommendations to policymakers and healthcare administrators, to support the implementation of HBC for patients.

Systematic review registration
Systematic review registration: PROSPERO CRD42024500476.
Temporal dynamics of leaf area index and land surface temperature correlation using Sentinel-2 and Landsat OLI data
Authors: Ahmed Ali Yasin , Ali Abebe Mohammed , Ahmed Nurhussen 
Published: September 2024

Abstract
Background
Understanding the complex relationship between vegetation dynamics and land surface temperature (LST) is crucial for comprehending ecosystem functioning, climate change impacts, and sustainable land management. Hence, this study conducts a temporal analysis of leaf area index (LAI) and LST data derived from Sentinel-2 and Landsat Operational Land Imagery (OLI) in the Mille River Basin, a tropical region in Ethiopia. LAI data were generated using Sentinel-2 imagery processed with the Sentinel Application Platform (SNAP) toolbox, an open-access earth observation analysis tool, while Landsat OLI collection 2 level 2 data were utilized for precise LST retrieval. The Mann–Kendall test was used to detect trends in the time series data.

Results
The trends in the mean LAI were statistically significant at P values of 0.05 and 0.10 for the annual and seasonal trends, respectively. The mean LST trends were insignificant throughout the study period except for the summer season, for which the P value was 0.07. The correlation between the LAI and LST was weak (R2 = 0.36) during the crop-growing seasons (summer and spring) but moderate in winter (R2 = 0.46) and autumn (R2 = 0.41).

Conclusion
The findings of this research clarify the complex relationships between variations in surface temperature and vegetation growth patterns, providing insight into the environmental mechanisms driving the dynamics of localized ecosystems. The study underscores the implications of these findings for informed decision-making in sustainable land management, biodiversity conservation, and climate change mitigation strategies.
Zero-crosstalk silicon photonic refractive index sensor with subwavelength gratings
Authors: Ahmed Syed Z. , Hasan Mehedi , Kim Kyungtae , Kim Sangsik 
Published: September 2024

AbstractSilicon photonic index sensors have received significant attention for label-free bio and gas-sensing applications, offering cost-effective and scalable solutions. Here, we introduce an ultra-compact silicon photonic refractive index sensor that leverages zero-crosstalk singularity responses enabled by subwavelength gratings. The subwavelength gratings are precisely engineered to achieve an anisotropic perturbation-led zero-crosstalk, resulting in a single transmission dip singularity in the spectrum that is independent of device length. The sensor is optimized for the transverse magnetic mode operation, where the subwavelength gratings are arranged perpendicular to the propagation direction to support a leaky-like mode and maximize the evanescent field interaction with the analyte space. Experimental results demonstrate a high wavelength sensitivity of − 410 nm/RIU and an intensity sensitivity of 395 dB/RIU, with a compact device footprint of approximately 82.8 μm2. Distinct from other resonant and interferometric sensors, our approach provides an FSR-free single-dip spectral response on a small device footprint, overcoming common challenges faced by traditional sensors, such as signal/phase ambiguity, sensitivity fading, limited detection range, and the necessity for large device footprints. This makes our sensor ideal for simplified intensity interrogation. The proposed sensor holds promise for a range of on-chip refractive index sensing applications, from gas to biochemical detection, representing a significant step towards efficient and miniaturized photonic sensing solutions.
Graphical Abstract
Repurposing metabolic regulators: antidiabetic drugs as anticancer agents
Authors: Dhas Yogita , Biswas Nupur , M.R. Divyalakshmi , Jones Lawrence D. , Ashili Shashaanka 
Published: September 2024

AbstractDrug repurposing in cancer taps into the capabilities of existing drugs, initially designed for other ailments, as potential cancer treatments. It offers several advantages over traditional drug discovery, including reduced costs, reduced development timelines, and a lower risk of adverse effects. However, not all drug classes align seamlessly with a patient's condition or long-term usage. Hence, repurposing of chronically used drugs presents a more attractive option. On the other hand, metabolic reprogramming being an important hallmark of cancer paves the metabolic regulators as possible cancer therapeutics. This review emphasizes the importance and offers current insights into the repurposing of antidiabetic drugs, including metformin, sulfonylureas, sodium-glucose cotransporter 2 (SGLT2) inhibitors, dipeptidyl peptidase 4 (DPP-4) inhibitors, glucagon-like peptide-1 receptor agonists (GLP-1RAs), thiazolidinediones (TZD), and α-glucosidase inhibitors, against various types of cancers. Antidiabetic drugs, regulating metabolic pathways have gained considerable attention in cancer research. The literature reveals a complex relationship between antidiabetic drugs and cancer risk. Among the antidiabetic drugs, metformin may possess anti-cancer properties, potentially reducing cancer cell proliferation, inducing apoptosis, and enhancing cancer cell sensitivity to chemotherapy. However, other antidiabetic drugs have revealed heterogeneous responses. Sulfonylureas and TZDs have not demonstrated consistent anti-cancer activity, while SGLT2 inhibitors and DPP-4 inhibitors have shown some potential benefits. GLP-1RAs have raised concerns due to possible associations with an increased risk of certain cancers. This review highlights that further research is warranted to elucidate the mechanisms underlying the potential anti-cancer effects of these drugs and to establish their efficacy and safety in clinical settings.