bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dislocação patelar aguda irredutível devido a uma nova variante anatômica - a patela entalhada
Authors: Duarte-Silva Miguel , Rodeia Joaquim , Gomes Tiago , Guerra-Pinto Francisco 
Published: December 2017

ResumoAs luxações irredutíveis da patela são raras e são geralmente associadas a mecanismos complexos. Os autores relatam o caso clínico de uma luxação patelar lateral irredutível devido a uma variante anatômica. Os autores atenderam um paciente de 16 anos que apresentou uma luxação lateral da patela de redução impossível por manipulação fechada, mesmo sob anestesia geral. Durante o estudo de imagem, a tomografia computadorizada (TC) mostrou um entalhe na faceta medial da patela, impactada no côndilo lateral, o que impediu a redução. Esta variante anatômica foi posteriormente confirmada durante a cirurgia. Em uma TC bilateral de acompanhamento, esta variante anatômica também estava presente no joelho contralateral, normal, excluindo o remodelamento traumático como o motivo deste entalhe patelar. Os autores utilizaram uma abordagem parapatelar medial para a redução aberta do deslocamento e para o reparo do retináculo medial. De acordo com Wiberg, existem três tipos diferentes de patela. Os autores descrevem uma variação da patela de tipo III com um entalhe na margem medial que não está incluída na classificação anterior. Ressalta-se a importância de um estudo de TC na presença de luxação irredutível e o reconhecimento desta variante anatômica da patela, já que manobras agressivas foram testadas sem sucesso. A redução aberta parece ser a melhor opção neste cenário.
Controllable deuteration of halogenated compounds by photocatalytic D2O splitting
Authors: Liu Cuibo , Chen Zhongxin , Su Chenliang , Zhao Xiaoxu , Gao Qiang , Ning Guo-Hong , Zhu Hai , Tang Wei , Leng Kai , Fu Wei , Tian Bingbing , Peng Xinwen , Li Jing , Xu Qing-Hua , Zhou Wu , Loh Kian Ping 
Published: January 2018

AbstractDeuterium labeling is of great value in organic synthesis and the pharmaceutical industry. However, the state-of-the-art C–H/C–D exchange using noble metal catalysts or strong bases/acids suffers from poor functional group tolerances, poor selectivity and lack of scope for generating molecular complexity. Herein, we demonstrate the deuteration of halides using heavy water as the deuteration reagent and porous CdSe nanosheets as the catalyst. The deuteration mechanism involves the generation of highly active carbon and deuterium radicals via photoinduced electron transfer from CdSe to the substrates, followed by tandem radicals coupling process, which is mechanistically distinct from the traditional methods involving deuterium cations or anions. Our deuteration strategy shows better selectivity and functional group tolerances than current C–H/C–D exchange methods. Extending the synthetic scope, deuterated boronic acids, halides, alkynes, and aldehydes can be used as synthons in Suzuki coupling, Click reaction, C–H bond insertion reaction etc. for the synthesis of complex deuterated molecules.
Author Correction: Ultrafast bridge planarization in donor-π-acceptor copolymers drives intramolecular charge transfer
Authors: Roy Palas , Jha Ajay , Yasarapudi Vineeth B. , Ram Thulasi , Puttaraju Boregowda , Patil Satish , Dasgupta Jyotishman 
Published: January 2018

The original version of this Article omitted the following from the Acknowledgements:“Satish Patil thanks Department of Science and Technology, New Delhi, India for a Swarnajayanti fellowship.”This has now been corrected in both the PDF and HTML versions of the Article.
A non-dispersion strategy for large-scale production of ultra-high concentration graphene slurries in water
Authors: Dong Lei , Chen Zhongxin , Zhao Xiaoxu , Ma Jianhua , Lin Shan , Li Mengxiong , Bao Yang , Chu Leiqiang , Leng Kai , Lu Hongbin , Loh Kian Ping 
Published: January 2018

AbstractIt is difficult to achieve high efficiency production of hydrophobic graphene by liquid phase exfoliation due to its poor dispersibility and the tendency of graphene sheets to undergo π−π stacking. Here, we report a water-phase, non-dispersion exfoliation method to produce highly crystalline graphene flakes, which can be stored in the form of a concentrated slurry (50 mg mL−1) or filter cake for months without the risk of re-stacking. The as-exfoliated graphene slurry can be directly used for 3D printing, as well as fabricating conductive graphene aerogels and graphene−polymer composites, thus avoiding the use of copious quantities of organic solvents and lowering the manufacturing cost. This non-dispersion strategy paves the way for the cost-effective and environmentally friendly production of graphene-based materials.
Linked cycles of oxidative decarboxylation of glyoxylate as protometabolic analogs of the citric acid cycle
Authors: Springsteen Greg , Yerabolu Jayasudhan Reddy , Nelson Julia , Rhea Chandler Joel , Krishnamurthy Ramanarayanan 
Published: January 2018

AbstractThe development of metabolic approaches towards understanding the origins of life, which have focused mainly on the citric acid (TCA) cycle, have languished—primarily due to a lack of experimentally demonstrable and sustainable cycle(s) of reactions. We show here the existence of a protometabolic analog of the TCA involving two linked cycles, which convert glyoxylate into CO2 and produce aspartic acid in the presence of ammonia. The reactions proceed from either pyruvate, oxaloacetate or malonate in the presence of glyoxylate as the carbon source and hydrogen peroxide as the oxidant under neutral aqueous conditions and at mild temperatures. The reaction pathway demonstrates turnover under controlled conditions. These results indicate that simpler versions of metabolic cycles could have emerged under potential prebiotic conditions, laying the foundation for the appearance of more sophisticated metabolic pathways once control by (polymeric) catalysts became available.
Large area growth of MoTe2 films as high performance counter electrodes for dye-sensitized solar cells
Authors: Hussain Sajjad , Patil Supriya A. , Vikraman Dhanasekaran , Mengal Naveed , Liu Hailiang , Song Wooseok , An Ki-Seok , Jeong Sung Hoon , Kim Hak-Sung , Jung Jongwan 
Published: January 2018

AbstractA cost effective and efficient alternative counter electrode (CE) to replace commercially existing platinum (Pt)-based CEs for dye-sensitized solar cells (DSSCs) is necessary to make DSSCs competitive. Herein, we report the large-area growth of molybdenum telluride (MoTe2) thin films by sputtering-chemical vapor deposition (CVD) on conductive glass substrates for Pt-free CEs of DSSCs. Cyclic voltammetry (CV), Tafel curve analysis, and electrochemical impedance spectroscopy (EIS) results showed that the as-synthesized MoTe2 exhibited good electrocatalytic properties and a low charge transfer resistance at the electrolyte-electrode interface. The optimized MoTe2 CE revealed a high power conversion efficiency of 7.25% under a simulated solar illumination of 100 mW cm−2 (AM 1.5), which was comparable to the 8.15% observed for a DSSC with a Pt CE. The low cost and good electrocatalytic properties of MoTe2 thin films make them as an alternative CE for DSSCs.
Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells
Authors: Hinderer Svenja , Sudrow Katrin , Schneider Maria , Holeiter Monika , Layland Shannon Lee , Seifert Martina , Schenke-Layland Katja 
Published: January 2018

AbstractDecorin (DCN) is an important small leucine-rich proteoglycan present in the extracellular matrix (ECM) of many organs and tissues. Endothelial progenitor cells (EPCs) are able to interact with the surrounding ECM and bind to molecules such as DCN. Here, we recombinantly produced full-length human DCN under good laboratory practice (GLP) conditions, and after detailed immunological characterization, we investigated its potential to attract murine and human EPCs (mEPCs and hECFCs). Electrospun polymeric scaffolds were coated with DCN or stromal cell-derived factor-1 (SDF-1α) and were then dynamically cultured with both cell types. Cell viability was assessed via imaging flow cytometry. The number of captured cells was counted and compared with the non-coated controls. To characterize cell-scaffold interactions, immunofluorescence staining and scanning electron microscopy analyses were performed. We identified that DCN reduced T cell responses and attracted innate immune cells, which are responsible for ECM remodeling. A significantly higher number of EPCs attached on DCN- and SDF-1α-coated scaffolds, when compared with the uncoated controls. Interestingly, DCN showed a higher attractant effect on hECFCs than SDF-1α. Here, we successfully demonstrated DCN as promising EPC-attracting coating, which is particularily interesting when aiming to generate off-the-shelf biomaterials with the potential of in vivo cell seeding.
Species-specific Posture of Human Foetus in Late First Trimester
Authors: Ohmura Yoshiyuki , Morokuma Seiichi , Kato Kiyoko , Kuniyoshi Yasuo 
Published: January 2018

AbstractThe ontogeny associated with the arm-hanging posture, which is considered ape-specific, remains unknown. To examine its ontogeny, we measured foetal movements of 62 human foetuses aged 10–20 gestation weeks using four-dimensional sonography. We observed that the first-trimester foetuses show this particular species-specific posture. After 11 weeks of gestation, all foetuses showed the arm-hanging posture, and the posture was most frequently observed at 14–16 weeks of gestation. Moreover, this posture often involved extension of both arms and both legs, indicating that it is not myogenic but neurogenic. Furthermore, early ontogeny suggests that it originates because of subcortical activity. Such posture extension bias and persistence indicates that vestibulospinal tract maturation involves the ontogeny of arm-hanging posture during 14–16 weeks of gestation.
Dial-in Topological Metamaterials Based on Bistable Stewart Platform
Authors: Wu Ying , Chaunsali Rajesh , Yasuda Hiromi , Yu Kaiping , Yang Jinkyu 
Published: January 2018

AbstractRecently, there have been significant efforts to guide mechanical energy in structures by relying on a novel topological framework popularized by the discovery of topological insulators. Here, we propose a topological metamaterial system based on the design of the Stewart Platform, which can not only guide mechanical waves robustly in a desired path, but also can be tuned in situ to change this wave path at will. Without resorting to any active materials, the current system harnesses bistablilty in its unit cells, such that tuning can be performed simply by a dial-in action. Consequently, a topological transition mechanism inspired by the quantum valley Hall effect can be achieved. We show the possibility of tuning in a variety of topological and traditional waveguides in the same system, and numerically investigate key qualitative and quantitative differences between them. We observe that even though both types of waveguides can lead to significant wave transmission for a certain frequency range, topological waveguides are distinctive as they support robust, back scattering immune, one-way wave propagation.
Variation in salinity tolerance between and within anadromous subpopulations of pike (Esox lucius)
Authors: Sunde Johanna , Tamario Carl , Tibblin Petter , Larsson Per , Forsman Anders 
Published: January 2018

AbstractEnvironmental heterogeneity is a key determinant of genetic and phenotypic diversity. Stable and homogenous environments tends to result in evolution of specialism and local adaptations, while temporally unpredictable environments may maintain a diversity of specialists, promote generalist strategies, or favour diversified bet hedging strategies. We compared salinity tolerance between two anadromous subpopulations of pike (Esox Lucius) that utilize freshwater spawning sites with different salinity regimes. Eggs from each population were artificially fertilized and incubated in a salinity gradient (0, 3, 5, 7, and 9 psu) using a split-brood design. Effects on embryonic development, hatching success, survival of larvae, and fry body length were compared between populations and families. The population naturally spawning in the stable freshwater habitat showed signs of specialization for freshwater spawning. The population exposed to fluctuating selective pressure in a spawning area with occasional brackish water intrusions tolerated higher salinities and displayed considerable variation in reaction norms. Genetic differences and plasticity of salinity tolerance may enable populations to cope with changes in salinity regimes associated with future climate change. That geographically adjacent subpopulations can constitute separate units with different genetic characteristics must be considered in management and conservation efforts to avoid potentially negative effects of genetic admixture on population fitness and persistence.
Assessment of Polysaccharides from Mycelia of genus Ganoderma by Mid-Infrared and Near-Infrared Spectroscopy
Authors: Ma Yuhan , He Huaqi , Wu Jingzhu , Wang Chunyang , Chao Kuanglin , Huang Qing 
Published: January 2018

AbstractGanoderma lingzhi (G. lingzhi), G. sinense, G. applanatum, etc. belongs to the Ganoderma genus of polypore mushrooms which contain rich polysaccharides valuable for nutrition and positive medicinal effects. In order to evaluate polysaccharide content in Ganoderma mycelia obtained in the fermentation process quickly and accurately, in this work we employed infrared spectroscopy to examine different Ganoderma stains of samples from diversified sources. Through mid-infrared (mid-IR) spectroscopy, we could identify the most relevant spectral bands required for polysaccharide evaluation, and through near-infrared (NIR) spectroscopy, we could establish the quantification model for making satisfactory prediction of polysaccharide ingredient content. As such, we have achieved an effective and convenient approach to quantitative assessment of the total polysaccharides in Ganoderma mycelia but also demonstrated that infrared spectroscopy can be a powerful tool for quality control of Ganoderma polysaccharides obtained from industrial production.
A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses
Authors: Saarinen Niila V. V. , Laiho Jutta E. , Richardson Sarah J. , Zeissler Marie , Stone Virginia M. , Marjomäki Varpu , Kantoluoto Tino , Horwitz Marc S. , Sioofy-Khojine Amirbabak , Honkimaa Anni , Hankaniemi Minna M. , Flodström-Tullberg Malin , Hyöty Heikki , Hytönen Vesa P. , Laitinen Olli H. 
Published: January 2018

AbstractEnteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybridoma clones were selected based on their reactivity in different immunoassays. The most promising clone, 3A6, was characterized and it performed well in multiple techniques including ELISA, immunoelectron microscopy, immunocyto- and histochemistry and in Western blotting, detecting EVs in infected cells and tissues. It recognized several EV-Bs and also the EV-C representative Poliovirus 3, making it a broad-spectrum EV specific antibody. The 3A6 rat monoclonal antibody can help to overcome some of the challenges faced with commonly used EV antibodies: it enables simultaneous use of mouse-derived antibodies in double staining and it is useful in murine models.
The gating mechanism in cyclic nucleotide-gated ion channels
Authors: Mazzolini Monica , Arcangeletti Manuel , Marchesi Arin , Napolitano Luisa M. R. , Grosa Debora , Maity Sourav , Anselmi Claudio , Torre Vincent 
Published: January 2018

AbstractCyclic nucleotide-gated (CNG) channels mediate transduction in several sensory neurons. These channels use the free energy of CNs’ binding to open the pore, a process referred to as gating. CNG channels belong to the superfamily of voltage-gated channels, where the motion of the α-helix S6 controls gating in most of its members. To date, only the open, cGMP-bound, structure of a CNG channel has been determined at atomic resolution, which is inadequate to determine the molecular events underlying gating. By using electrophysiology, site-directed mutagenesis, chemical modification, and Single Molecule Force Spectroscopy, we demonstrate that opening of CNGA1 channels is initiated by the formation of salt bridges between residues in the C-linker and S5 helix. These events trigger conformational changes of the α-helix S5, transmitted to the P-helix and leading to channel opening. Therefore, the superfamily of voltage-gated channels shares a similar molecular architecture but has evolved divergent gating mechanisms.
Exactly solvable model of two trapped quantum particles interacting via finite-range soft-core interactions
Authors: Kościk Przemysław , Sowiński Tomasz 
Published: January 2018

AbstractThe exactly solvable model of two indistinguishable quantum particles (bosons or fermions) confined in a one-dimensional harmonic trap and interacting via finite-range soft-core interaction is presented and many properties of the system are examined. Particularly, it is shown that independently on the potential range, in the strong interaction limit bosonic and fermionic solutions become degenerate. For sufficiently large ranges a specific crystallization appears in the system. The results are compared to predictions of the celebrated Busch et al. model and those obtained in the Tonks-Girardeau limit. The assumed inter-particle potential is very similar to the potential between ultra-cold dressed Rydberg atoms. Therefore, the model can be examined experimentally.
Determinants of E2-ubiquitin conjugate recognition by RBR E3 ligases
Authors: Martino Luigi , Brown Nicholas R. , Masino Laura , Esposito Diego , Rittinger Katrin 
Published: January 2018

AbstractRING-between-RING (RBR) ubiquitin ligases work with multiple E2 enzymes and function through an E3-ubiquitin thioester intermediate. The RBR module comprises three domains, RING1, IBR and RING2 that collaborate to transfer ubiquitin from the E2~Ub conjugate, recognised by RING1, onto a catalytic cysteine in RING2 and finally onto the substrate in a multi-step reaction. Recent studies have shown that RING1 domains bind E2~Ub conjugates in an open conformation to supress ubiquitin transfer onto lysine residues and promote formation of the E3 thioester intermediate. However, how the nature of the E2 influences the ubiquitin transfer process is currently unclear. We report here a detailed characterization of the RBR/E2-conjugate recognition step that indicates that this mechanism depends on the nature of the E2 enzyme and differs between UbcH5 and UbcH7. In the case of UbcH5~Ub an interaction with ubiquitin is necessary to stabilize the transfer complex while recognition of UbcH7~Ub is driven primarily by E2-RING1 contacts. Furthermore our analysis suggests that RBRs, in isolation and in complex with ubiquitin-loaded E2s, are dynamic species and that their intrinsic flexibility might be a key aspect of their catalytic mechanism.
Characterisation of the antibacterial properties of the recombinant phage endolysins AP50-31 and LysB4 as potent bactericidal agents against Bacillus anthracis
Authors: Park Sangjin , Jun Soo Youn , Kim Chang-Hwan , Jung Gi Mo , Son Jee Soo , Jeong Seong Tae , Yoon Seong Jun , Lee Sang Yup , Kang Sang Hyeon 
Published: January 2018

AbstractThe recombinant phage endolysins AP50-31 and LysB4 were developed using genetic information from bacteriophages AP50 and B4 and were produced by microbial cultivation followed by chromatographic purification. Subsequently, appropriate formulations were developed that provided an acceptable stability of the recombinant endolysins. The bacteriolytic properties of the formulated endolysins AP50-31 and LysB4 against several bacterial strains belonging to the Bacillus genus including Bacillus anthracis (anthrax) strains were examined. AP50-31 and LysB4 displayed rapid bacteriolytic activity and broad bacteriolytic spectra within the Bacillus genus, including bacteriolytic activity against all the B. anthracis strains tested. When administered intranasally, LysB4 completely protected A/J mice from lethality after infection with the spores of B. anthracis Sterne. When examined at 3 days post-infection, bacterial counts in the major organs (lung, liver, kidney, and spleen) were significantly lower compared with those of the control group that was not treated with endolysin. In addition, histopathological examinations revealed a marked improvement of pathological features in the LysB4-treated group. The results of this study support the idea that phage endolysins are promising candidates for developing therapeutics against anthrax infection.
A Novel Dual-Step Nucleation Pathway in Crystalline Solids under Neutron Irradiation
Authors: Meher Subhashish , van Rooyen Isabella J. , Lillo Thomas M. 
Published: January 2018

AbstractInnovations in nanostructuring of inorganic crystalline solids are often limited by prerequisite critical nucleation energy and solute supersaturation for formation of a phase. This research provides direct evidence supporting the viability of an unconventional irradiation-induced nanostructuring process, via transmission electron microscopy, that circumvents these preconditions. Using polymorphic silicon carbide (SiC) as a prototype, a surprising two-step nucleation route is demonstrated through which nanoscale distribution of the second phase is achieved by reaction of solutes with neutron irradiation-induced precursors. In the first step, nanoscale α–SiC precipitates in a β–SiC matrix unexpectedly nucleate heterogeneously at structural defects. This occurs at significantly lower temperatures compared with the usual β→α transition temperature. Subsequently, α–SiC precipitate acts as a surrogate template for its structural and compositional transition into a fission product precipitate, palladium silicide. These discoveries provide a modern view of irradiation engineering in polymorphic ceramics for advanced applications.
Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A2A Receptor crystals
Authors: Rucktooa Prakash , Cheng Robert K. Y. , Segala Elena , Geng Tian , Errey James C. , Brown Giles A. , Cooke Robert M. , Marshall Fiona H. , Doré Andrew S. 
Published: January 2018

AbstractHere we report an efficient method to generate multiple co-structures of the A2A G protein-coupled receptor (GPCR) with small-molecules from a single preparation of a thermostabilised receptor crystallised in Lipidic Cubic Phase (LCP). Receptor crystallisation is achieved following purification using a low affinity “carrier” ligand (theophylline) and crystals are then soaked in solutions containing the desired (higher affinity) compounds. Complete datasets to high resolution can then be collected from single crystals and seven structures are reported here of which three are novel. The method significantly improves structural throughput for ligand screening using stabilised GPCRs, thereby actively driving Structure-Based Drug Discovery (SBDD).