bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production Quality Improvements and Energy Cost Reductions Associated With Compressed Air
Authors: Krisa Mark , Voelker David 
Published: January 2018

Compressed air is utilized throughout various production processes in the citrus industry and can influence production quality and operating costs. Within production equipment, compressed air is expanded from a higher pressure to perform various tasks. The pressure ahead of the final discharge location can have a direct impact on the operation of the specific process. Article pressure is the term used to describe the pressure located closest to the point where air is expanded to do work. Article pressure can be influenced by many variables that exist between supply equipment (compressors) and the point of use. Understanding the relationship between the supply pressure and the article pressure will facilitate the ability to maximize the repeatability and performance of production equipment and minimize the supply power required to operate the compressed air system.
This paper will discuss variables that influence the difference between the pressure supplied by the compressor station and the pressure utilized within the production equipment. Illustrations and field examples will be utilized to describe issues. Troubleshooting methods will be discussed along with a description of how to trend variables that influence production so problems can be corrected before they influence productivity.
Paper published with permission.
Aseptic Ingredient Addition: Meeting the Demand for Better-Tasting Orange Juice
Authors: Brocker Paul P. 
Published: January 2018

Since the late 1970’s, Aseptic Not-From-Concentrate Orange Juice (NFCOJ) has been successfully stored in large refrigerated aseptic storage tanks. Aseptic tanks have evolved from 280,000 gallons in volume to now in excess of 1.8 million gallons each. The total bulk storage capacity in Florida has grown to approximately 280 millions of gallons and continues to grow with new installations occurring each year at some facilities. Worldwide, the market is expanding into Brazil, Spain, and markets that are beginning to receive juice shipped in bulk on snips. The aseptic storage methods have been accepted in Brazil and Europe, and aseptic transfer of the juice is occurring via specially outfitted aseptic tanker vessels from Brazil to the US and Europe.
The consumer’s demand for NFCOJ has grown steadily throughout these years, and the suppliers of consumer packaged orange juice have developed special processes and methods to maximize the quality and flavor of the juices sent to the market. Fresh juice, light pasteurization, and flavor enhanced products are just some of these methods resulting in very high quality juice availability. Also, cost and price are always under assault, and the juice suppliers are always looking for an edge. Recently, the flavor enhancement method has come under scrutiny by the FDA, and the industry is being reminded that all added flavors must be made from naturally occurring orange derivatives or must be labeled appropriately: such as “with natural (other fruit) flavors” or “with artificial flavors,” both of which may have an undesirable impact on the market perception of the juice quality.
At this same time, as the bulk storage technology of NFCOJ has matured in the past 25 years, some processors who package their own juice are investing in special aseptic transfer methods from the aseptic bulk storage tanks without the need to re-pasteurize the juice prior to packaging. Their goal is to provide the highest quality juice to the consumer, and to minimize or eliminate the need to add expensive and special flavor packs to the juice. This is being done commercially in Florida and Spain.
This paper explores these methods of aseptic juice transfer direct to packaging and the aseptic addition of natural or otherwise desired and labeled ingredients, and their potential impact on the quality of the juice.
Paper published with permission.
Crossflow Filtration (Ceramic and Hollow Fiber Membranes) in the Citrus Industry
Authors: Sondhi Rishi 
Published: January 2018

Crossflow filtration is becoming a cost-effective and preferred method of separation in the food and beverage industry. This paper introduces the concept of crossflow filtration and describes the different membrane technologies and materials. Additionally, advances in ceramic tubular and polymeric hollow fiber membrane technology are presented. New membrane configurations and their practical impact in the industry are discussed. Typical filtration applications in the citrus industry such as fruit juice clarification, clean-in-place (CIP) solutions recovery, and water recovery applications are highlighted.
Paper published with permission.
Caustic Recovery Using Membrane Filtration
Authors: Grigus Mike 
Published: January 2018

Food and beverage processors that use dilute caustic solutions for cleaning process equipment have shown increased interest in recovering the used caustic. The primary reason is that the price of caustic has increased significantly in the past year or so. Membrane filtration technology can be used to remove suspended solids (clarify with microfiltration) and/or dissolved solids (purify with nanofiltration) from these used caustic solutions. These treated caustic solutions are suitable for reuse within the processing plant as cleaning solutions. While an end-user’s specific process, performance, capital and operating cost parameters require pilot testing and process design evaluations to determine the economic viability of caustic recovery, the existing body of information from commercial and process development work allows for an evaluation of a “typical” caustic recovery application.
Paper published with permission.
Water Conservation and Wastewater Load Reduction in Citrus Processing
Authors: Ashby James 
Published: January 2018

In the state of Florida, the vast majority of potable water comes from groundwater supplies. Salt water intrusion into groundwater aquifers coupled with variable rainfall and periodic droughts are affecting the availability of this supply. As the supply of potable groundwater in Florida becomes less and less available, it is crucial for municipalities and industry alike to take the initiative to protect diminishing water resources through conservation.
In addition, increased scrutiny from state and federal regulators on effluent wastewater discharges from industrial facilities such as citrus processing plants has resulted in tighter effluent discharge standards, limits to organic loadings on land application and increased wastewater pretreatment requirements. These trends are expected to continue and citrus processors are faced with the reality that these tightening requirements are making wastewater treatment an even more critical part of their operations.
Paper published with permission.
Draft Genome Sequences of 510
Listeria monocytogenes
Strains from Food Isolates and Human Listeriosis Cases from Northern Italy
Authors: Lomonaco Sara , Gallina Silvia , Filipello Virginia , Sanchez Leon Maria , Kastanis George John , Allard Marc , Brown Eric , Amato Ettore , Pontello Mirella , Decastelli Lucia 
Published: January 2018

ABSTRACT

Listeriosis outbreaks are frequently multistate/multicountry outbreaks, underlining the importance of molecular typing data for several diverse and well-characterized isolates. Large-scale whole-genome sequencing studies on
Listeria monocytogenes
isolates from non-U.S. locations have been limited. Herein, we describe the draft genome sequences of 510
L. monocytogenes
isolates from northern Italy from different sources.
Draft Genome Sequences of Two
Vibrio parahaemolyticus
Strains Associated with Gastroenteritis after Raw Seafood Ingestion in Colorado
Authors: Trinh Sonya A. , Leyn Semen A. , Rodionov Ivan D. , Godzik Adam , Satchell Karla J. F. 
Published: January 2018

ABSTRACT

Vibrio parahaemolyticus
is a Gram-negative pathogen associated with gastrointestinal and wound infections after exposure to raw seafood or contaminated waters. We report here the whole-genome sequences of two stool isolates (CDC-AM50933 and CDC-AM43539) from patients in Colorado presenting with gastroenteritis after ingesting raw seafood.
Draft Genome Sequence of
Escherichia coli
Strain SN137, a Bacterium with Extracellular Proteolytic Activity on Immunoglobulins and Persistence in Human Tissue Blood
Authors: Najera-Hernandez Salustio , Sanchez-Alonso Maria Patricia , Anastacio-Marcelino Estela , Negrete-Abascal Erasmo , Vazquez-Cruz Candelario 
Published: January 2018

ABSTRACT

The draft genome sequence of
Escherichia coli
strain SN137 is reported here. The genome comprises 172 contigs, corresponding to 4.9 Mb with 50% G+C content, and contains several genes related to pathogenicity that explain its survival in human hematic tissue.
Draft Genome Sequence of
Bacillus velezensis
OSY-S3, a Producer of Potent Antimicrobial Agents Active against Bacteria and Fungi
Authors: Gerst Michelle M. , Yesil Mustafa , Yousef Ahmed E. 
Published: January 2018

ABSTRACT

Bacillus velezensis
OSY-S3 produces anti-
Listeria
, anti-
Escherichia coli
, and antifungal compounds. Additionally, fermentate of
B. velezensis
OSY-S3 culture removes
Staphylococcus aureus
biofilms effectively. The draft genome sequence of
B. velezensis
OSY-S3 reported here had a genome size of ~3.90 Mb and a G+C content of 46.5%.
Complete Genome Sequence of
Lacinutrix venerupis
DOK2-8 Isolated from Marine Sediment from the East Sea, Republic of Korea
Authors: Lim Se Ra , Jeong Dae Gwin , Chi Won-Jae , Kim Ji Hyung 
Published: January 2018

ABSTRACT

Lacinutrix venerupis
has recently been considered a potential fish pathogen. Here, we report the complete genome sequence of
L. venerupis
DOK2-8, which possesses several virulence-related genes. This strain may be potentially virulent to other marine organisms, and its genomic information will provide important insights into the biodiversity of the genus
Lacinutrix
.
Repression of VvpM Protease Expression by Quorum Sensing and the cAMP-cAMP Receptor Protein Complex in Vibrio vulnificus
Authors: Kim Jeong-A , Lee Mi-Ae , Jung You-Chul , Jang Bo-Ram , Lee Kyu-Ho 
Published: January 2018

ABSTRACT

Septicemia-causing
Vibrio vulnificus
produces at least three exoproteases, VvpE, VvpS, and VvpM, all of which participate in interactions with human cells. Expression of VvpE and VvpS is induced in the stationary phase by multiple transcription factors, including sigma factor S, SmcR, and the cAMP-cAMP receptor protein (cAMP-CRP) complex. Distinct roles of VvpM, such as induction of apoptosis, lead us to hypothesize VvpM expression is different from that of the other exoproteases. Its transcription, which was found to be independent of sigma S, is induced at the early exponential phase and then becomes negligible upon entry into the stationary phase. SmcR and CRP were studied regarding the control of
vvpM
expression. Transcription of
vvpM
was repressed by SmcR and cAMP-CRP complex individually, which specifically bound to the regions −2 to +20 and +6 to +27, respectively, relative to the
vvpM
transcription initiation site. Derepression of
vvpM
gene expression was 10- to 40-fold greater in an
smcR crp
double mutant than in single-gene mutants. Therefore, these results show that the expression of
V. vulnificus
exoproteases is differentially regulated, and in this way, distinct proteases can engage in specific interactions with a host.

IMPORTANCE
An opportunistic human pathogen,
Vibrio vulnificus
produces multiple extracellular proteases that are involved in diverse interactions with a host. The total exoproteolytic activity is detected mainly in the supernatants of the high-cell-density cultures. However, some proteolytic activity derived from a metalloprotease, VvpM, was present in the supernatants of the low-cell-density cultures sampled at the early growth period. In this study, we present the regulatory mechanism for VvpM expression via repression by at least two transcription factors. This type of transcriptional regulation is the exact opposite of those for expression of the other
V. vulnificus
exoproteases. Differential regulation of each exoprotease's production then facilitates the pathogen's participation in the distinct interactions with a host.
RASA1/NF1-Mutant Lung Cancer: Racing to the Clinic?
Authors: Kitajima Shunsuke , Barbie David A. 
Published: January 2018

Abstract
Although mutation of NF1 has been described in non–small cell lung cancer (NSCLC), co-mutation with RASA1, another Ras-GTPase activating protein (RasGAP), defines a novel genetically defined subclass of NSCLC. RASA1/NF1-mutant cell lines are highly sensitive to MEK inhibitors, warranting clinical evaluation of MAPK inhibition in this subclass of patients. Clin Cancer Res; 24(6); 1243–5. ©2018 AACR.
See related article by Hayashi et al., p. 1436
Relative Target Affinities of T-Cell–Dependent Bispecific Antibodies Determine Biodistribution in a Solid Tumor Mouse Model
Authors: Mandikian Danielle , Takahashi Nene , Lo Amy A. , Li Ji , Eastham-Anderson Jeffrey , Slaga Dionysos , Ho Jason , Hristopoulos Maria , Clark Robyn , Totpal Klara , Lin Kedan , Joseph Sean B. , Dennis Mark S. , Prabhu Saileta , Junttila Teemu T. , Boswell C. Andrew 
Published: January 2018

Abstract
Anti-HER2/CD3, a T-cell–dependent bispecific antibody (TDB) construct, induces T-cell–mediated cell death in cancer cells expressing HER2 by cross-linking tumor HER2 with CD3 on cytotoxic T cells, thereby creating a functional cytolytic synapse. TDB design is a very challenging process that requires consideration of multiple parameters. Although therapeutic antibody design strategy is commonly driven by striving for the highest attainable antigen-binding affinity, little is known about how the affinity of each TDB arm can affect the targeting ability of the other arm and the consequent distribution and efficacy. To our knowledge, no distribution studies have been published using preclinical models wherein the T-cell–targeting arm of the TDB is actively bound to T cells. We used a combined approach involving radiochemistry, invasive biodistribution, and noninvasive single-photon emission tomographic (SPECT) imaging to measure TDB distribution and catabolism in transgenic mice with human CD3ϵ expression on T cells. Using CD3 affinity variants, we assessed the impact of CD3 affinity on short-term pharmacokinetics, tissue distribution, and cellular uptake. Our experimental approach determined the relative effects of (i) CD3 targeting to normal tissues, (ii) HER2 targeting to HER2-expressing tumors, and (iii) relative HER2/CD3 affinity, all as critical drivers for TDB distribution. We observed a strong correlation between CD3 affinity and distribution to T-cell–rich tissues, with higher CD3 affinity reducing systemic exposure and shifting TDB distribution away from tumor to T-cell–containing tissues. These observations have important implications for clinical translation of bispecific antibodies for cancer immunotherapy. Mol Cancer Ther; 17(4); 776–85. ©2018 AACR.
Tumor Immunity and Survival as a Function of Alternative Neopeptides in Human Cancer
Authors: Rech Andrew J. , Balli David , Mantero Alejandro , Ishwaran Hemant , Nathanson Katherine L. , Stanger Ben Z. , Vonderheide Robert H. 
Published: January 2018

Abstract
The immune system exerts antitumor activity via T cell–dependent recognition of tumor-specific antigens. Although the number of tumor neopeptides—peptides derived from somatic mutations—often correlates with immune activity and survival, most classically defined high-affinity neopeptides (CDNs) are not immunogenic, and only rare CDNs have been linked to tumor rejection. Thus, the rules of tumor antigen recognition remain incompletely understood. Here, we analyzed neopeptides, immune activity, and clinical outcome from 6,324 patients across 27 tumor types. We characterized a class of “alternatively defined neopeptides” (ADNs), which are mutant peptides predicted to bind MHC (class I or II) with improved affinity relative to their nonmutated counterpart. ADNs are abundant and molecularly distinct from CDNs. The load of ADNs correlated with intratumoral T-cell responses and immune suppression, and ADNs were also strong predictors of patient survival across tumor types. These results expand the spectrum of mutation-derived tumor antigens with potential clinical relevance. Cancer Immunol Res; 6(3); 276–87. ©2018 AACR.
Sensitive Detection of Atrial Fibrillation in Acute Stroke Patients by Short-Term Bedside Electrocardiography Monitoring Software Analysis
Authors: Ross Linda Sarah , Bettin Markus , Kochhäuser Simon , Ritter Martin , Minnerup Jens , Eckardt Lars , Reinke Florian , Dittrich Ralf 
Published: January 2018

<b><i>Background:</i></b> Atrial fibrillation (AF) is an important cause of stroke. Continuous electrocardiography (ECG) monitoring with software-based analysis algorithms has been suggested to enhance the AF detection rate. We investigated the ability of stroke risk analysis (SRA) in the detection of AF in acute stroke patients. <b><i>Methods:</i></b> Consecutive stroke patients numbering 1,153 were screened. Patients with cardioembolic stroke related to AF (<i>n</i> = 296, paroxysmal <i>n</i> = 63, persistent <i>n</i> = 233) and patients with cryptogenic stroke (<i>n</i> = 309) after standard diagnostic work-up (bedside ECG monitoring, ultrasound, transesophageal echocardiography, 24 h Holter ECG) received SRA during their stay at the Stroke Unit. Determination of AF risk by SRA in the patients with AF and in the patient group with cryptogenic stroke was assessed and compared. <b><i>Results:</i></b> Median SRA monitoring analysis time was 16 h (range 2–206 h, interquartile range 10–36). In AF patients, SRA also detected a possible or definitive AF in 98%. The overall sensitivity of SRA to detect possible or definitive AF in patients with proven AF by standard diagnostic work up and cryptogenic stroke was 98%, specificity 27%, positive predictive value 56%, and the negative predictive value (NPV) was 92%. Area under ROC curve was 0.622. <b><i>Conclusion:</i></b> SRA was found to be highly sensitive to detect possible or definitive AF in clinical routine within a short monitoring time. However, low specificity and poor accuracy do not allow diagnosing AF by SRA alone, but with the high NPV compared to current diagnostic standard, it is a valid diagnostic tool to rule out AF. Thereby, SRA is a contribution to clarify stroke etiology.
JUDICIALIZAÇÃO DA SAÚDE E INTERNAÇÕES COMPULSÓRIAS DE JOVENS USUÁRIOS DE DROGAS – UM ESTUDO EM ESPÍRITO SANTO DO PINHAL/SP
Authors: Bartolomei Josiara Rabello , Rezende Laura Ferreira de 
Published: January 2018

O artigo tem por objetivo analisar processos de internação compulsória de jovens e adultos usuários de drogas relativos aos anos de 2009 a 2014 na cidade de Espírito Santo do Pinhal (SP). Tratou-se de um estudo longitudinal retrospectivo, de caráter exploratório documental. O presente trabalho revelou que, em 99 processos, as famílias buscam na Justiça a internação compulsória de seus familiares. As internações de adolescentes via ação judicial são feitas em clínicas particulares pagas pelo município, enquanto os adultos são internados pelo Sistema Único de Saúde. Não há transferência de recursos estaduais, e os recursos federais não cobrem os gastos. Não há políticas públicas para esse fim, e há necessidade de fortalecimento dos Centros de Atenção Psicossocial para promoção da sustentabilidade e do equilíbrio social.
Breve recorrido por la literatura española del siglo XIX. De los románticos hasta la Generación del 98
Authors: Hernandéz Aline Kelly Vieira 
Published: January 2018

No século XIX, o homem europeu encontra-se inflamado pelas ideias e sentimentos de liberdade, igualdade e fraternidade pregados pela Revolução francesa. Na Espanha, essas ideias e sentimentos são potencializados pela derrota das forças napoleônicas durante a Guerra de Independência espanhola. Toda essa gama de fortes emoções está plasmada na literatura da primeira metade do século. O Romantismo espanhol reflete todo esse sentimento de rebeldia, nacionalismo e necessidade de exaltação dos sentimentos, aliado ao desejo de viver intensamente. Na segunda metade do século, o Realismo espanhol surge como contraponto às ideias românticas. O Realismo apresenta-se como forma de observação da sociedade e seus costumes. Segundo os realistas, era preciso retratar a vida da forma como ela realmente acontece e não de maneira fantasiosa. Ao final do século, como uma maneira de reagir ao sentimento de decadência que se proliferava na Espanha, causado pelo chamado “Desastre do 98”, surge a “Geração do 98”, formada por um grupo de jovens escritores espanhóis preocupados pelo futuro e rumo que tomaria sua nação. No presente trabalho, temos como objetivo oferecer um panorama geral da literatura espanhola do século XIX. Não podendo colocar no trabalho todos os autores e obras deste século, tomamos como base alguns dos principais representantes de cada época e movimento literário. Os resultados deste trabalho destinam-se a colaborar em sua medida para os estudos literários sobre a literatura espanhola do século XIX.
Modeling the Impact of Interhospital Transfer Network Design on Stroke Outcomes in a Large City
Authors: Parikh Neal S. , Chatterjee Abhinaba , Díaz Iván , Pandya Ankur , Merkler Alexander E. , Gialdini Gino , Kummer Benjamin R. , Mir Saad A. , Lerario Michael P. , Fink Matthew E. , Navi Babak B. , Kamel Hooman 
Published: January 2018

Background and Purpose—
We sought to model the effects of interhospital transfer network design on endovascular therapy eligibility and clinical outcomes of stroke because of large-vessel occlusion for the residents of a large city.

Methods—
We modeled 3 transfer network designs for New York City. In model A, patients were transferred from spoke hospitals to the closest hub hospitals with endovascular capabilities irrespective of hospital affiliation. In model B, which was considered the base case, patients were transferred to the closest affiliated hub hospitals. In model C, patients were transferred to the closest affiliated hospitals, and transfer times were adjusted to reflect full implementation of streamlined transfer protocols. Using Monte Carlo methods, we simulated the distributions of endovascular therapy eligibility and good functional outcomes (modified Rankin Scale score, 0–2) in these models.

Results—
In our models, 200 patients (interquartile range [IQR], 168–227) with a stroke amenable to endovascular therapy present to New York City spoke hospitals each year. Transferring patients to the closest hub hospital irrespective of affiliation (model A) resulted in 4 (IQR, 1–9) additional patients being eligible for endovascular therapy and an additional 1 (IQR, 0–2) patient achieving functional independence. Transferring patients only to affiliated hospitals while simulating full implementation of streamlined transfer protocols (model C) resulted in 17 (IQR, 3–41) additional patients being eligible for endovascular therapy and 3 (IQR, 1–8) additional patients achieving functional independence.

Conclusions—
Optimizing acute stroke transfer networks resulted in clinically small changes in population-level stroke outcomes in a dense, urban area.