bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparing Nursing Students to Work With Patients With Alcohol or Drug-Related Problems
Authors: Nash Angela J. , Marcus Marianne T. , Cron Stanley , Scamp Nadine , Truitt Morgan , McKenna Zara 
Published: August 2017

Abstract
Stigma and insufficient training for addressing alcohol or drug problems in clinical settings are two significant barriers to earlier identification and access to care for patients with alcohol or drug (AOD)-related problems. Nurses are crucial players in this strategy, yet nurses often report poor motivation and insufficient training for working with AOD-affected patients. This article describes and reports preliminary evaluation results of an educational experience that integrated AOD curriculum into a community/public health nursing clinical practicum. The purpose of the intervention was to meet the required objectives for our university’s community/public health nursing clinical course, improve students’ attitudes and therapeutic commitment for working with AOD-affected patients, and prepare students for providing high-quality nursing care for individuals with AOD-related problems.
Topics of Addiction in Turkish Nursing Programs
Authors: Ozturk Candan , Ayar Dijle , Arkan Burcu , Fougere Merry Armstrong 
Published: August 2017

Abstract
Addiction is a serious health issue. The measured rates of substance abuse in Turkey are lower than those in Western countries; however, recent studies show a significant increase in the prevalence of substance abuse and that the age of onset of substance abuse has decreased. There is a lack of literature about the topic of addiction in the undergraduate nursing programs in Turkey. Therefore, the aim of this current study was to examine the presence of addiction content in nursing education in Turkey among state and foundation universities.
A descriptive, survey research design was used in this study. One hundred twelve state universities and 64 foundation universities that provide undergraduate education in Turkey were screened using the database of the Council of Higher Education. Researchers found that 89.09% of the state universities and all the foundation universities included the topic of addiction and/or substance addiction somewhere in their undergraduate nursing programs. The courses were primarily elective courses, and availability of the coursework varied. Given the impact of substance and process addictions, the authors conclude with a recommendation that the topic of addiction be offered as a discrete, mandatory course in the undergraduate nursing curricula of all universities offering nursing programs.
Recovery Beyond Buprenorphine
Authors: Fogger Susanne A. , Lehmann Kathleen 
Published: August 2017

Abstract
It has taken the tragedy of swelling opioid overdoses to raise addictions to national attention. This past year, a new law called the Comprehensive Addictions Recovery Act has helped to open doors for nurse practitioners and physician assistants to prescribe buprenorphine. Although this medication can assist those dependent on opioids in finding needed stability, medication-assisted treatment is only the beginning. Addiction and psychiatric nurses must play a larger role in providing various therapies that coincide with medication-assisted treatment to support ongoing recovery. One treatment option is group therapy, which is effective for individuals with substance use as well as other co-occurring disorders to develop needed skills to remain in recovery. The purpose of this review is to explore the nursing role in group therapy for substance use as well as encourage addictions and psychiatric nurse practitioners to offer recovery-focused group therapy to this population.
An Evolutionary Route for the Moral Development of Nursing Students: A Constructivist Grounded Theory
Authors: RANJBAR Hadi , JOOLAEE Soodabeh , VEDADHIR Abouali , ABBASSZADEH Abbass , BERNSTEIN Colleen 
Published: August 2017

ABSTRACT

Background:
Developing moral competency is a main aim of educational systems, especially in healthcare-related disciplines.

Purpose:
The aim of this study was to explore the moral development process in nursing students.

Methods:
This study used a constructivist grounded theory. Twenty-five semistructured, face-to-face interview with 22 participants were conducted to gather the data. The participants were nursing students and educators attending three nursing schools in Tehran.

Results:
Moral development evolves within nursing students following a stepwise progression. Within each step or phase, students experience specific changes with regard to both their practical skills and their moral capabilities and competencies. Three main steps were identified: moral transition, moral reconstruction, and moral internalization. Techno-scientific competence, biomedical competence, and nursing competence are developed in each step, respectively.

Conclusions/Implications for Practice:
Moral competency in nursing students develops along with their competency as nursing practitioners. To facilitate progress through each phase, it needs to be ensured that the learning experiences that students are exposed to during each consecutive year of study facilitate as much as possible the development of moral competence.
Factors Facilitating Publication by Clinical Nurses in a Magnet® Hospital
Authors: Tyndall Deborah E. , Scott Elaine S. , Caswell Nicole I. 
Published: August 2017

OBJECTIVE
This study examined the cognitive, behavioral, and environmental factors that facilitate publication by clinical nurses in Magnet® hospitals.

BACKGROUND
The culture promoted by the Magnet Recognition Program® promotes nurses practicing in those settings to use, generate, and disseminate best practices. Successful and promising models of care and nursing practice are rarely disseminated beyond the organization where they are practiced. The questions persist: what barriers to writing for publication exist for clinical nurses, and how do we overcome them to advance emergent evidence?

METHODS
A focused ethnographic, multiple-case study design examined 5 well-published nurses with high levels of dissemination occurring outside the Magnet organization where they were employed.

RESULTS
Nurses in this study most often credited cognitive and behavioral factors for promoting publication. Although the organization encouraged peer-reviewed publication, there were limited environmental efforts to support efforts to publish in peer-reviewed journals by clinical nurses.

CONCLUSIONS
The results of this case study call for more research on the multifaceted factors that promote publication by clinical nurses in the practice environment. Publication of research in peer-reviewed journals was supported by environmental influences of time to write, collaborative writing opportunities, and supportive and accurate guidance from editors.
TRIP6 inhibits the Hippo signaling pathway in response to tension at adherens junctions
Authors: Dutta Shubham , Mana-Capelli Sebastian , Paramasivam Murugan , Dasgupta Ishani , Cirka Heather , Billiar Kris , McCollum Dannel 
Published: August 2017

AbstractThe transcriptional co-activator YAP controls cell proliferation, survival, and tissue regeneration in response to changes in the mechanical environment. It is not known how mechanical stimuli such as tension are sensed and how the signal is transduced to control YAP activity. Here we show that the LIM domain protein TRIP6 acts as part of a mechanotransduction pathway at adherens junctions to promote YAP activity by inhibiting the LATS1/2 kinases. Previous studies showed that vinculin at adherens junctions becomes activated by mechanical tension. We show that vinculin inhibits Hippo signaling by recruiting TRIP6 to adherens junctions and stimulating its binding to and inhibition of LATS1/2 in response to tension. TRIP6 competes with MOB1 for binding to LATS1/2 thereby blocking MOB1 from recruiting the LATS1/2 activating kinases MST1/2. Together these findings reveal a novel mechanotransduction cascade that transduces tension signals sensed at adherens junctions to control Hippo pathway signaling.
ApoplastP: prediction of effectors and plant proteins in the apoplast using machine learning
Authors: Sperschneider Jana , Dodds Peter N. , Singh Karam B. , Taylor Jennifer M. 
Published: August 2017

AbstractThe plant apoplast is integral to intercellular signalling, transport and plant-pathogen interactions. Plant pathogens deliver effectors both into the apoplast and inside host cells, but no computational method currently exists to discriminate between these localizations. We present ApoplastP, the first method for predicting if an effector or plant protein localizes to the apoplast. ApoplastP uncovers features for apoplastic localization common to both effectors and plant proteins, namely an enrichment in small amino acids and cysteines as well as depletion in glutamic acid. ApoplastP predicts apoplastic localization in effectors with sensitivity of 75% and false positive rate of 5%, improving accuracy of cysteine-rich classifiers by over 13%. ApoplastP does not depend on the presence of a signal peptide and correctly predicts the localization of unconventionally secreted plant and effector proteins. The secretomes of fungal saprophytes, necrotrophic pathogens and extracellular pathogens are enriched for predicted apoplastic proteins. Rust pathogen secretomes have the lowest percentage of apoplastic proteins, but these are highly enriched for predicted effectors. ApoplastP pioneers apoplastic localization prediction using machine learning. It will facilitate functional studies and will be valuable for predicting if an effector localizes to the apoplast or if it enters plant cells. ApoplastP is available athttp://apoplastp.csiro.au.
DNA methylation profiling of breast cancer cell lines along the epithelial mesenchymal spectrum - implications for the choice of circulating tumour DNA methylation markers
Authors: Le Anh Viet-Phuong , Szaumkessel Marcin , Tan Tuan Zea , Thiery Jean-Paul , Thompson Erik W , Dobrovic Alexander 
Published: August 2017

AbstractEpithelial-mesenchymal plasticity (EMP) is a dynamic process whereby epithelial carcinoma cells reversibly acquire morphological and invasive characteristics typical of mesenchymal cells, which facilitates metastasis. Understanding the methylation differences between epithelial and mesenchymal states may assist in the identification of optimal DNA methylation biomarkers for the blood-based monitoring of cancer. Methylation-sensitive high-resolution melting (MS-HRM) was used to examine the promoter methylation status of a panel of established and novel markers in a range of breast cancer cell lines spanning the epithelial-mesenchymal spectrum. Pyrosequencing was used to validate the MS-HRM results. The results indicate an overall distinction in methylation between epithelial and mesenchymal phenotypes. The mesenchymal expression markersVIM, DKK3andCRABP1were methylated in the majority of epithelial breast cancer cell lines while methylation of the epithelial expression markersGRHL2, MIR200CandCDH1was restricted to mesenchymal cell lines. We also examined EMP association of several methylation markers that have been used to assess minimal residual disease. Markers such asAKR1B1andAPCmethylation proved to be selective for epithelial breast cell lines, howeverRASSF1A, RARß, TWIST1andSFRP2methylation was seen in both epithelial and mesenchymal cell lines, supporting their suitability for a multi-marker panel.
Phosphatase PP2A and microtubule pulling forces disassemble centrosomes during mitotic exit
Authors: Enos Stephen J. , Dressler Martin , Gomes Beatriz Ferreira , Hyman Anthony A. , Woodruff Jeffrey B. 
Published: August 2017

ABSTRACTCentrosomes are major microtubule-nucleating organelles that facilitate chromosome segregation and cell division in metazoans. Centrosomes comprise centrioles that organize a micron-scale mass of protein called pericentriolar material (PCM) from which microtubules nucleate. During each cell cycle, PCM accumulates around centrioles through phosphorylation-mediated assembly of PCM scaffold proteins. During mitotic exit, PCM swiftly disassembles by an unknown mechanism. Here, we usedCaenorhabditis elegansembryos to determine the mechanism and importance of PCM disassembly in dividing cells. We found that the phosphatase PP2A and its regulatory subunit SUR-6 (PP2ASUR-6), together with cortically directed microtubule pulling forces, actively disassemble PCM. In embryos depleted of these activities, ~25% of PCM persisted from one cell cycle into the next, resulting in cytokinetic furrow ingression errors, excessive centrosome accumulation, and embryonic death. Purified pp2ASUR-6could dephosphorylate the major PCM scaffold protein SPD-5in vitro. Our data suggest that PCM disassembly occurs through a combination of dephosphorylation of PCM components and catastrophic rupture of the PCM scaffold.
A Mathematical Model of the Phosphoinositide Pathway
Authors: Olivença Daniel V , Uliyakina Inna , Fonseca Luis L , Amaral Margarida D , Voit Eberhard , Pinto Francisco R 
Published: August 2017

ABSTRACTPhosphoinositides are signaling lipids that constitute a complex network regulating many cellular processes. We propose a computational model that accounts for all known species of phosphoinositides in the plasma membrane of mammalian cells. The model replicates the steady-state of the phosphoinositide pathway and most known dynamic phenomena. Furthermore, sensitivity analysis demonstrates model robustness to moderate alterations in any of the parameters. Model analysis suggest that the greatest contributor to PI(4,5)P2production is a flux representing the direct transformation of PI into PI(4,5)P2and is also responsible for the maintenance of this pool when PI(4)P is decreased. PI(5)P is also shown to be a significant source for PI(4,5)P2production. The model was validated with data from siRNA screens that knocked down the expression of several enzymes in the pathway. The screen monitored the activity of the epithelium sodium channel, ENaC, which is activated by PI(4,5)P2. Moderating ENaC activity can have a therapeutic effect in Cystic Fibrosis (CF) patients. Our model suggests control strategies where the activities of the enzyme PIP5KI or the PI4K+PIP5KI+DVL protein complex are decreased and cause an efficacious reduction in PI(4,5)P2levels while avoiding undesirable alterations in other phosphoinositide pools.AbbreviationsAKTProtein Kinase B, a serine/threonine-specific protein kinaseASLAirway surface liquidBSTBiochemical systems theoryCFCystic fibrosisDAGDiacylglycerolENaCEpithelial Sodium ChannelEREndoplasmic ReticulumGMAGeneralized mass actionINPP5Inositol polyphosphate 5-phosphatasesIP3Inositol triphosphateLTPslipid transport proteinsMCSsmembrane contact sitesMDCK cellsMadin-Darby Canine Kidney Epithelial CellsMTMMyotubularinOCRLLowe Oculocerebrorenal Syndrome Protein; OCRL is an INPP5ODEOrdinary differential equationsPIPhosphatidylinositolPI(3)Pphosphatidylinositol 3-phosphatePI(3,4)P2Phosphatidylinositol 3,4-biphosphatePI(3,4,5)P3phosphatidylinositol 3,4,5-triphosphate, with phosphates in the third, fourth and fifth positionsPI(3,5)P2Phosphatidylinositol 3,5-biphosphatePI(4)Pphosphatidylinositol 4-phosphatePI(4,5)P2Phosphatidylinositol 4,5-biphosphate with phosphates in the fourth and fifth positions of the inositol ringPI(5)Pphosphatidylinositol 5-phosphatePI3KPhosphoinositide 3-kinasePI4KPhosphoinositide 4-kinasePIP5KPhosphoinositide 4-phosphate 5-kinasePIKfyveFYVE finger-containing phosphoinositide kinase.PLCPhospholipase CPLDPhospholipase DPLIPPTEN-like lipid phosphatasePTENPhosphatase and tensin homologPKCProtein kinase CSACSuppressor of actinSHIP1SH2 domain-containing phosphatidylinositol 5’-phosphataseSKIPSkeletal muscle and kidney enriched inositol polyphosphate phosphataseSYNJSynaptojaninsTPIPPTEN-Like Inositol Lipid PhosphataseWnt3aWingless-Type MMTV Integration Site Family, Member 3ADVLSegment Polarity Protein Dishevelled Homolog DVL
Quantitation of brain tumour microstructure response to Temozolomide therapy using non-invasive VERDICT MRI
Authors: Roberts Tom A. , Hyare Harpreet , Agliardi Giulia , Hipwell Ben , d’Esposito Angela , Ianus Andrada , Breen-Norris James O. , Ramasawmy Rajiv , Taylor Valerie , Atkinson David , Punwani Shonit , Lythgoe Mark F. , Siow Bernard , Brandner Sebastian , Rees Jeremy , Panagiotaki Eleftheria , Alexander Daniel C. , Walker-Samuel Simon 
Published: August 2017

ABSTRACTThere has been slow progress in the development of new therapeutic strategies for treating brain tumours, partly because assessment of treatment response is difficult and largely reliant on simple bi-dimensional measurements of MRI contrast-enhancing regions. Hence, there is a clinical need to develop improved imaging techniques for monitoring treatment response. In this study, we evaluate VERDICT (Vascular, Extracellular and Restricted Diffusion for Cytometry in Tumors) MRI in mouse glioblastomas for the quantification of tumour microstructure and assessment of response to Temozolomide (TMZ) chemotherapy, and, we investigate the feasibility of applying VERDICT MRI in a range of human gliomas. VERDICT MRI detected response to TMZ earlier than structural and apparent diffusion coefficient (ADC) measurements. A significant reduction in the cell radius parameter was detected three days earlier than ADC and six days earlier than structural MRI. Histological analysis showed the same trend as VERDICT of decreased intracellular volume fraction in the TMZ-treated mice. Vascular volume fraction was not altered by TMZ, which was consistent with optical projection tomography measurements. In patients, glioblastoma compartmental volume fractions showed good agreement with mouse glioblastoma parameters. The VERDICT parameters varied across the human gliomas, with raised intracellular volume fraction in the oligodendrogliomas and elevated cell radius in both low-grade tumours subtypes. In conclusion, our results suggest that VERDICT MRI is more sensitive at detecting TMZ response than structural or ADC measurements. In patients, VERDICT is feasible within clinical scan times, and performed best at characterising glioblastoma. Further optimisation should improve assessment of different glioma subtypes.
Molecular phylogenetics supports a clade of red algal parasites retaining native plastids: taxonomy and terminology revised
Authors: Salomaki Eric D. , Lane Christopher E. 
Published: August 2017

AbstractParasitism is a life strategy that has repeatedly evolved within the Florideophyceae. Historically, the terms adelphoparasite and alloparasite have been used to distinguish parasites based on the relative phylogenetic relationship of host and parasite. However, analyses using molecular phylogenetics indicate that nearly all red algal parasites infect within their taxonomic family, and a range of relationships exist between host and parasite. To date, all investigated adelphoparasites have lost their plastid, and instead, incorporate a host derived plastid when packaging spores. In contrast, a highly reduced plastid lacking photosynthesis genes was sequenced from the alloparasiteChoreocolax polysiphoniae. Here we present the completeHarveyella mirabilisplastid genome, which has also lost genes involved in photosynthesis, and a partial plastid genome fromLeachiella pacifica. TheH. mirabilisplastid shares more synteny with free-living red algal plastids than that ofC. polysiphoniae. Phylogenetic analysis demonstrates thatC. polysiphoniae, H. mirabilis, andL. pacificaform a robustly supported clade of parasites, which retain their own plastid genomes, within the Rhodomelaceae. We therefore transfer all three genera from the exclusively parasitic family, Choreocolacaceae, to the Rhodomelaceae. Additionally, we recommend applying the terms archaeplastic parasites (formerly alloparasites), and neoplastic parasites (formerly adelphoparasites) to distinguish red algal parasites using a biological framework rather than taxonomic affiliation with their hosts.
Ectodomain of plasmodesmata-localized protein 5 inArabidopsis: expression, purification, crystallization and crystallographic analysis
Authors: Wang Xiaocui , Zhu Peiyan , Qu Shanshan , Zhao Jie , Singh Prashant K. , Wang Wei 
Published: August 2017

Plasmodesmata-localized protein 5 (PDLP5) is a cysteine-rich receptor-like protein which is localized on the plasmodesmata ofArabidopsis thaliana. Overexpression of PDLP5 can reduce the permeability of the plasmodesmata and further affect the cell-to-cell movement of viruses and macromolecules in plants. The ectodomain of PDLP5 contains two DUF26 domains; however, the function of these domains is still unknown. Here, the ectodomain of PDLP5 fromArabidopsiswas cloned and overexpressed using an insect expression system and was then purified and crystallized. X-ray diffraction data were collected to 1.90 Å resolution and were indexed in space groupP1, with unit-cell parametersa= 41.9,b= 48.1,c = 62.2 Å, α = 97.3, β = 103.1, γ = 99.7°. Analysis of the crystal content indicated that there are two molecules in the asymmetric unit, with a Matthews coefficient of 2.51 Å3 Da−1and a solvent content of 50.97%.
Spice boys: an exploratory study around novel psychoactive substance use on a male acute ward
Authors: Place Charlie , Scally Andy , Gow Laura , Wade Amy , Barrowcliff Rob , Nasim Iram , Nyamuchiwa Miriam 
Published: August 2017

Purpose
Novel psychoactive substances (NPS) – often known as “legal highs” – are a varied group of substances that are causing concern due to their possible effects on mental health. Particular concerns have been raised about synthetic cannabinoid receptor agonists often known as “Spice”. The purpose of this paper is to identify the prevalence of NPS use and explore any association with acute psychological disturbance.

Design/methodology/approach
The authors reviewed the case notes for 153 admissions to a male acute inpatient ward. Prevalence of reported NPS use and admissions to psychiatric intensive care unit (PICU) were recorded.

Findings
In total, 18.9 per cent of individuals admitted to acute inpatient care were reported to have used NPS. NPS users were almost ten times as likely as their non-NPS using peers to require care in PICU. This result was statistically significant (Fisher exact test: p<0.001). Although caution is required given the limitations of this study, the data and clinical experience suggest that synthetic cannabinoids may be the specific type of NPS that is being used by this group.

Practical implications
Mental health professionals can expect to care for people using NPS in acute inpatient environments, and so they need to understand the nature and effects of these substances. It is possible that NPS use may be associated with sustained acute psychological disturbance.

Originality/value
There have been few studies on the prevalence of NPS use in inpatient environments and none of them have published that explore the association with PICU admission. Despite the limitations of this study, it has significant value by identifying an urgent need for comprehensive research in this area.