bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coparenting dynamics: Mothers' and fathers' differential support and involvement
Authors: MURPHY SARAH E. , GALLEGOS MARTIN I. , JACOBVITZ DEBORAH B. , HAZEN NANCY L. 
Published: November 2017

AbstractThis study utilizes the actor–partner interdependence model to examine mothers' and fathers' support of their partner and involvement in parental decision making during coparenting interactions in relation to cooperative and competitive coparenting in a sample of 125 first‐time parents with a 24‐month‐old child. Fathers showed greater instances of support for their partner than did mothers, and mothers demonstrated higher levels of involvement in parenting decisions than did fathers. Mothers' higher support of fathers' parenting was related negatively to competitive coparenting and positively to fathers' involvement. Fathers' higher support of mothers and higher involvement in parenting decisions was related to higher cooperative coparenting. Implications for family intervention and future research are discussed.
Is Inequality Underestimated in Egypt? Evidence from House Prices
Authors: van der Weide Roy , Lakner Christoph , Ianchovichina Elena 
Published: November 2017

AbstractHousehold income surveys often fail to capture top incomes, which leads to an underestimation of income inequality. A popular solution is to combine the household survey with data from income tax records, which has been found to result in significant upward corrections of inequality estimates. Unfortunately, tax records are unavailable in many countries, including most of the developing world. In the absence of data from tax records, this study explores the feasibility of using data on house prices to estimate the top tail of the income distribution. In an application to Egypt, where estimates of inequality based on household surveys alone are low by international standards, the study finds strong evidence that inequality is indeed being underestimated by a considerable margin. The Gini index of household per capita income for urban Egypt is found to increase from 39 to 52 after correcting for the missing top tail.
Disability, social functioning and school inclusion among older children and adolescents living with HIV in Zimbabwe
Authors: Rukuni Ruramayi , McHugh Grace , Majonga Edith , Kranzer Katharina , Mujuru Hilda , Munyati Shungu , Nathoo Kusum , Gregson Celia L. , Kuper Hannah , Ferrand Rashida A. 
Published: November 2017

AbstractObjectiveIncreasing numbers of children with HIV are surviving to adolescence and encountering multiple clinical and social consequences of long‐standing HIV infection. We aimed to investigate the association between HIV and disability, social functioning and school inclusion among 6‐ to 16‐year‐olds in Zimbabwe.MethodsHIV‐infected children receiving antiretroviral therapy from a public‐sector HIV clinic and HIV‐uninfected children attending primary care clinics in the same catchment area were recruited. Standardised questionnaires were used to collect socio‐demographic, social functioning and disability data. Multivariable logistic regression was used to assess the relationship between HIV status and disability and functioning.ResultsWe recruited 202 HIV‐infected and 285 HIV‐uninfected children. There was no difference in age and gender between the two groups, but a higher proportion of HIV‐infected children were orphaned. The prevalence of any disability was higher in HIV‐infected than uninfected children (37.6% vs. 18.5%, P < 0.001). HIV‐infected children were more likely to report anxiety (adjusted odds ratio (aOR) 4.4; 95% CI 2.4, 8.1), low mood (aOR 4.2; 2.1, 8.4) and difficulty forming friendships (aOR 14.8; 1.9, 116.6) than uninfected children. Children with HIV also reported more missed school days, repeating a school year and social exclusion in class. These associations remained apparent when comparing children with HIV and disability to those with HIV but no disabilities.ConclusionsChildren with HIV commonly experience disabilities, and this is associated with social and educational exclusion. Rehabilitation and support services are needed to facilitate educational attainment and social participation in this group.
Apple fruit superficial scald resistance mediated by ethylene inhibition is associated with diverse metabolic processes
Authors: Busatto Nicola , Farneti Brian , Commisso Mauro , Bianconi Martino , Iadarola Barbara , Zago Elisa , Ruperti Benedetto , Spinelli Francesco , Zanella Angelo , Velasco Riccardo , Ferrarini Alberto , Chitarrini Giulia , Vrhovsek Urska , Delledonne Massimo , Guzzo Flavia , Costa Guglielmo , Costa Fabrizio 
Published: November 2017

SummaryFruits stored at low temperature can exhibit different types of chilling injury. In apple, one of the most serious physiological disorders is superficial scald, which is characterized by discoloration and brown necrotic patches on the fruit exocarp. Although this phenomenon is widely ascribed to the oxidation of α‐farnesene, its physiology is not yet fully understood. To elucidate the mechanism of superficial scald development and possible means of prevention, we performed an integrated metabolite screen, including an analysis of volatiles, phenols and lipids, together with a large‐scale transcriptome study. We also determined that prevention of superficial scald, through the use of an ethylene action inhibitor, is associated with the triggering of cold acclimation‐related processes. Specifically, the inhibition of ethylene perception stimulated the production of antioxidant compounds to scavenge reactive oxygen species, the synthesis of fatty acids to stabilize plastid and vacuole membranes against cold temperature, and the accumulation of the sorbitol, which can act as a cryoprotectant. The pattern of sorbitol accumulation was consistent with the expression profile of a sorbitol 6‐phosphate dehydrogenase, MdS6PDH, the overexpression of which in transgenic Arabidopsis thaliana plants confirmed its involvement in the cold acclimation and freezing tolerance.
The soluble guanylate cyclase CYG12 is required for the acclimation to hypoxia and trophic regimes in Chlamydomonas reinhardtii
Authors: Düner Melis , Lambertz Jan , Mügge Carolin , Hemschemeier Anja 
Published: November 2017

SummaryOxygenic phototrophs frequently encounter environmental conditions that result in intracellular energy crises. Growth of the unicellular green alga Chlamydomonas reinhardtii in hypoxia in the light depends on acclimatory responses of which the induction of photosynthetic cyclic electron flow is essential. The microalga cannot grow in the absence of molecular oxygen (O2) in the dark, although it possesses an elaborate fermentation metabolism. Not much is known about how the microalga senses and signals the lack of O2 or about its survival strategies during energy crises. Recently, nitric oxide (NO) has emerged to be required for the acclimation of C. reinhardtii to hypoxia. In this study, we show that the soluble guanylate cyclase (sGC) CYG12, a homologue of animal NO sensors, is also involved in this response. CYG12 is an active sGC, and post‐transcriptional down‐regulation of the CYG12 gene impairs hypoxic growth and gene expression in C. reinhardtii. However, it also results in a disturbed photosynthetic apparatus under standard growth conditions and the inability to grow heterotrophically. Transcriptome profiles indicate that the mis‐expression of CYG12 results in a perturbation of responses that, in the wild‐type, maintain the cellular energy budget. We suggest that CYG12 is required for the proper operation of the photosynthetic apparatus which, in turn, is essential for survival in hypoxia and darkness.
Public willingness to pay a premium for uni‐material beverage container in Korea: a contingent valuation study
Authors: Park So‐Yeon , Lim Seul‐Ye , Yoo Seung‐Hoon 
Published: November 2017

AbstractThe use of uni‐materials can reduce the total waste generation by a high recycling rate. This study assesses the public's willingness to pay (WTP) a premium for the uni‐material beverage container (UBC) in Korea using a specific case of bottled mineral water in polyethylene terephthalate (PET). For this purpose, a contingent valuation (CV) survey of 1000 consumers was conducted to derive the additional WTP for the mineral water in UBC instead of that in non‐UBC. Given that the average price of the mineral water (500 mL) in non‐UBC was KRW 1000 (USD 0.88), the mean additional WTP a premium for that in UBC was estimated to be KRW 107 (USD 0.09). This value amounts to 10.7% of price of the mineral water in non‐UBC and can be interpreted as the public value of the UBC. The consumers in Korea are ready to pay a significant premium for UBCs.
Application of response surface methodology to optimize nitrate removal at low temperature by aerobic denitrificator Pseudomonas strain An‐1
Authors: Ren He‐Jun , Su Yu , Wang Cong , Hou Zhi‐Min , Zhou Ye , Zhou Rui 
Published: November 2017

AbstractResponse surface methodology (RSM) was applied to optimize the conditions of nitrate removal by Pseudomonas strain AN‐1 at low temperature, which was isolated from aniline‐contaminated groundwater. A three‐factor, three‐level Box–Behnken experimental design and analysis of variance were combined with the obtained second‐order polynomial equation and applied for RSM optimization. By solving the second‐order polynomial equation and analysing the 3D response surface, a
‐N concentration of 65.29 mg/L, an initial pH of 6.65, and an initial DO of 7.53 were identified as the optimized conditions. These conditions achieved a removal percentage of 53.7485% after 48 h at 10°C, which was in good agreement with the predicted 53.8085%. This study showed the excellent ability of the Pseudomonas strain AN‐1 to degrade nitrate at low temperatures, which provided a solid foundation for the further application of this microorganism in the treatment of nitrate‐contaminated groundwater.
Activation of voltage‐dependent K+ channels strongly limits hypoxia‐induced elevation of [Ca2+]i in rat carotid body glomus cells
Authors: Wang Jiaju , Kim Donghee 
Published: November 2017

Key points
We studied the role of the large‐conductance Ca2+‐activated K+ channel (BK) and voltage‐dependent K+ channels (Kv) on [Ca2+]i responses to a wide range of hypoxia at different resting cell membrane potential (Em).
BK/Kv were mostly closed at rest in normoxia. BK/Kv became basally active when cells were depolarized by elevated [KCl]o (>12 mm). Regardless of whether BK/Kv were closed or basally open, hypoxia‐induced elevation of [Ca2+]i was enhanced 2‐ to 3‐fold by inhibitors of BK/Kv.
Hypoxia‐induced elevation of [Ca2+]i was enhanced ∼2‐fold by an inhibitor of Kv2, a major Kv in rat glomus cells.
Hypoxia did not inhibit BK in inside‐out patches.
Our study supports a scheme in which activation of BK/Kv strongly limits the magnitude of hypoxia‐induced [Ca2+]i rise, with Kv having a much greater effect than BK.
AbstractLarge‐conductance KCa (BK) and other voltage‐dependent K+ channels (Kv) are highly expressed in carotid body (CB) glomus cells, but their role in hypoxia‐induced excitation is still not well defined and remains controversial. We addressed this issue by studying the effects of inhibitors of BK (IBTX) and BK/Kv (TEA/4‐AP) on [Ca2+]i responses to a wide range of hypoxia at different levels of resting cell membrane potential (Em). IBTX and TEA/4‐AP did not affect the basal [Ca2+]i in isolated glomus cells bathed in 5 mm KClo, but elicited transient increases in [Ca2+]i in cells that were moderately depolarized (11–20 mV) by elevation of [KCl]o (12–20 mm). Thus, BK and Kv were mostly closed at rest and activated by depolarization. Four different levels of hypoxia (mild, moderate, severe, anoxia) were used to produce a wide range of [Ca2+]i elevation (0–700 nm). IBTX did not affect the rise in [Ca2+]i, but TEA/4‐AP strongly (∼3‐fold) enhanced [Ca2+]i rise by moderate and severe levels of hypoxia. Guangxitoxin, a Kv2 blocker, inhibited the whole‐cell current by ∼50%, and enhanced 2‐fold the [Ca2+]i rise elicited by moderate and severe levels of hypoxia. Anoxia did not directly affect BK, but activated BK via depolarization. Our findings do not support the view that hypoxia inhibits BK/Kv to initiate or maintain the hypoxic response. Rather, our results show that BK/Kv are activated as glomus cells depolarize in response to hypoxia, which then limits the rise in [Ca2+]i. Inhibition of Kv may provide a mechanism to enhance the chemosensory activity of the CB and ventilation.
Community Composition and Ultrastructure of a Nitrate-Dependent Anaerobic Methane-Oxidizing Enrichment Culture
Authors: Gambelli Lavinia , Guerrero-Cruz Simon , Mesman Rob J. , Cremers Geert , Jetten Mike S. M. , Op den Camp Huub J. M. , Kartal Boran , Lueke Claudia , van Niftrik Laura 
Published: November 2017

ABSTRACT

Methane is a very potent greenhouse gas and can be oxidized aerobically or anaerobically through microbe-mediated processes, thus decreasing methane emissions in the atmosphere. Using a complementary array of methods, including phylogenetic analysis, physiological experiments, and light and electron microscopy techniques (including electron tomography), we investigated the community composition and ultrastructure of a continuous bioreactor enrichment culture, in which anaerobic oxidation of methane (AOM) was coupled to nitrate reduction. A membrane bioreactor was seeded with AOM biomass and continuously fed with excess methane. After 150 days, the bioreactor reached a daily consumption of 10 mmol nitrate · liter
−1
· day
−1
. The biomass consisted of aggregates that were dominated by nitrate-dependent anaerobic methane-oxidizing “
Candidatus
Methanoperedens”-like archaea (40%) and nitrite-dependent anaerobic methane-oxidizing “
Candidatus
Methylomirabilis”-like bacteria (50%). The “
Ca
. Methanoperedens” spp. were identified by fluorescence
in situ
hybridization and immunogold localization of the methyl-coenzyme M reductase (Mcr) enzyme, which was located in the cytoplasm. The “
Ca
. Methanoperedens” sp. aggregates consisted of slightly irregular coccoid cells (∼1.5-μm diameter) which produced extruding tubular structures and putative cell-to-cell contacts among each other. “
Ca
. Methylomirabilis” sp. bacteria exhibited the polygonal cell shape typical of this genus. In AOM archaea and bacteria, cytochrome
c
proteins were localized in the cytoplasm and periplasm, respectively, by cytochrome staining. Our results indicate that AOM bacteria and archaea might work closely together in the process of anaerobic methane oxidation, as the bacteria depend on the archaea for nitrite. Future studies will be aimed at elucidating the function of the cell-to-cell interactions in nitrate-dependent AOM.

IMPORTANCE
Microorganisms performing nitrate- and nitrite-dependent anaerobic methane oxidation are important in both natural and man-made ecosystems, such as wastewater treatment plants. In both systems, complex microbial interactions take place that are largely unknown. Revealing these microbial interactions would enable us to understand how the oxidation of the important greenhouse gas methane occurs in nature and pave the way for the application of these microbes in wastewater treatment plants. Here, we elucidated the microbial composition, ultrastructure, and physiology of a nitrate-dependent AOM community of archaea and bacteria and describe the cell plan of “
Ca
. Methanoperedens”-like methanotrophic archaea.
Complete Mitochondrial Genome Sequence of the Human Neuroblastoma Cell Line 751-NA
Authors: Zhang Mao , Chen Jian-Long , Wang Peng-Cheng , Fu Chuan-Yi , Peng Hao 
Published: November 2017

ABSTRACT

We report here for the first time the 16,566-bp mitochondrial genome sequence of the human neuroblastoma cell line 751-NA. The 13 protein-coding genes, 2 rRNAs, and 22 tRNAs are organized in a virtually identical fashion to a previously reported human mitochondrial genome, except that the 1,136-bp
d
-loop region is slightly variable between them.
Whole-Genome Shotgun Sequences of Three Multidrug-Resistant Mycobacterium tuberculosis Strains Isolated from Morocco
Authors: Lahlou Lamiaa , El Mrimar Nadia , Laamarti Meriem , Alouane Tarek , Bendahou Mohammed A. , Bssaibis Fatna , Ben Lahlou Yassine , Zegmout Adil , El Hafidi N. , ElJaoudi Rachid , Frikh Mohammed , Lemnouar Abdelhay , Elouennass Mostafa , Ibrahimi Azeddine 
Published: November 2017

ABSTRACT

Tuberculosis is a contagious disease that usually attacks the lungs but sometimes attacks other parts of the body, such as the kidneys, glands, and bones. It is an endemic and major public health problem in Morocco. Tuberculosis is transmitted through the airways via the inhalation of microdroplets containing
Mycobacterium tuberculosis
. We present here the whole-genome shotgun sequences of three multidrug-resistant
M. tuberculosis
strains isolated from Morocco.
Complete Genome Sequence of a Novel Nonnodulating
Rhizobium
Species Isolated from
Agave americana
L. Rhizosphere
Authors: Ruíz-Valdiviezo Víctor Manuel , Rogel-Hernandez Marco Antonio , Guerrero Gabriela , Rincón-Molina Clara Ivette , García-Perez Luis Galdino , Gutiérrez-Miceli Federico Antonio , Villalobos-Maldonado Juan José , López-López Aline , Martinez-Romero Esperanza , Rincón-Rosales Reiner 
Published: November 2017

ABSTRACT

We report here the complete genome sequence of
Rhizobium
sp. strain ACO-34A, isolated from
Agave americana
L. rhizosphere. No common
nod
genes were found, but there were
nif
genes for nitrogen fixing. A low average nucleotide identity to reported species supports its designation as a novel
Rhizobium
species that has a complete ribosomal operon in a plasmid.
Complete Genome Sequence of Faecalibacterium prausnitzii Isolated from the Gut of a Healthy Indian Adult
Authors: Bag Satyabrata , Ghosh Tarini Shankar , Das Bhabatosh 
Published: November 2017

ABSTRACT

Faecalibacterium prausnitzii
is the most abundant (~4%) member of the phylum
Firmicutes
found in the colon of healthy humans. It is a strict anaerobe and plays an important role in intestinal homeostasis. Here, we report the complete genome sequence of
F. prausnitzii
strain Indica.
Draft Genome Sequence of the Naturally Competent Bacillus simplex Strain WY10
Authors: Keen Eric C. , Bliskovsky Valery V. , Adhya Sankar L. , Dantas Gautam 
Published: November 2017

ABSTRACT

We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. Sequence analysis revealed that WY10 is a novel strain of
Bacillus simplex
. To our knowledge, WY10 is the first
B. simplex
strain to be characterized as naturally competent for DNA uptake by transformation.
Low alcohol consumption and pregnancy and childhood outcomes: time to change guidelines indicating apparently ‘safe’ levels of alcohol during pregnancy? A systematic review and meta-analyses
Authors: Mamluk Loubaba , Edwards Hannah B , Savović Jelena , Leach Verity , Jones Timothy , Moore Theresa H M , Ijaz Sharea , Lewis Sarah J , Donovan Jenny L , Lawlor Debbie , Smith George Davey , Fraser Abigail , Zuccolo Luisa 
Published: November 2017

ObjectivesTo determine the effects of low-to-moderate levels of maternal alcohol consumption in pregnancy on pregnancy and longer-term offspring outcomes.Search strategyMedline, Embase, Web of Science and Psychinfo from inception to 11 July 2016.Selection criteriaProspective observational studies, negative control and quasiexperimental studies of pregnant women estimating effects of light drinking in pregnancy (≤32 g/week) versus abstaining. Pregnancy outcomes such as birth weight and features of fetal alcohol syndrome were examined.Data collection and analysisOne reviewer extracted data and another checked extracted data. Random effects meta-analyses were performed where applicable, and a narrative summary of findings was carried out otherwise.Main results24 cohort and two quasiexperimental studies were included. With the exception of birth size and gestational age, there was insufficient data to meta-analyse or make robust conclusions. Odds of small for gestational age (SGA) and preterm birth were higher for babies whose mothers consumed up to 32 g/week versus none, but estimates for preterm birth were also compatible with no association: summary OR 1.08, 95% CI (1.02 to 1.14), I20%, (seven studies, all estimates were adjusted) OR 1.10, 95% CI (0.95 to 1.28), I260%, (nine studies, includes one unadjusted estimates), respectively. The earliest time points of exposure were used in the analysis.ConclusionEvidence of the effects of drinking ≤32 g/week in pregnancy is sparse. As there was some evidence that even light prenatal alcohol consumption is associated with being SGA and preterm delivery, guidance could advise abstention as a precautionary principle but should explain the paucity of evidence.
Survival after bone metastasis by primary cancer type: a Danish population-based cohort study
Authors: Svensson Elisabeth , Christiansen Christian F , Ulrichsen Sinna P , Rørth Mikael R , Sørensen Henrik T 
Published: November 2017

ObjectiveIn the 10 most common primary types with bone metastases, we aimed to examine survival, further stratifying on bone metastases only or with additional synchronous metastases.MethodsWe included all patients aged 18 years and older with incident hospital diagnosis of solid cancer between 1994 and 2010, subsequently diagnosed with BM until 2012. We followed patients from date of bone metastasis diagnosis until death, emigration or 31 December 2012, whichever came first. We computed 1-year, 3-year and 5-year survival (%) and the corresponding 95% CIs stratified on primary cancer type. Comparing patients with bone metastasis only and patients with other synchronous metastases, we estimated crude and adjusted HRs and corresponding 95% CI for mortality.ResultsWe included 17 251 patients with bone metastasis. The most common primary cancer types with bone metastasis were prostate (34%), breast (22%) and lung (20%). One-year survival after bone metastasis diagnosis was lowest in patients with lung cancer (10%, 95% CI 9% to 11%) and highest in patients with breast cancer (51%, 50% to 53%). At 5 years of follow-up, only patients with breast cancer had over 10% survival (13%, 11% to 14%). The risk of mortality was increased for the majority of cancer types among patients with bone and synchronous metastases compared with bone only (adjusted relative risk 1.29–1.57), except for cervix, ovarian and bladder cancer.ConclusionsWhile patients with bone metastases after most primary cancers have poor survival, one of ten patients with bone metastasis from breast cancer survived 5 years.