bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of Heterologous Mevalonic Acid Pathway Enzymes in Clostridium ljungdahlii for the Conversion of Fructose and of Syngas to Mevalonate and Isoprene
Authors: Diner Bruce A. , Fan Janine , Scotcher Miles C. , Wells Derek H. , Whited Gregory M. 
Published: October 2017

ABSTRACT

There is a growing interest in the use of microbial fermentation for the generation of high-demand, high-purity chemicals using cheap feedstocks in an environmentally friendly manner. One example explored here is the production of isoprene (C
5
H
8
), a hemiterpene, which is primarily polymerized to polyisoprene in synthetic rubber in tires but which can also be converted to C
10
and C
15
biofuels. The strictly anaerobic, acetogenic bacterium
Clostridium ljungdahlii
, used in all of the work described here, is capable of glycolysis using the Embden-Meyerhof-Parnas pathway and of carbon fixation using the Wood-Ljungdahl pathway.
Clostridium
-
Escherichia coli
shuttle plasmids, each bearing either 2 or 3 different heterologous genes of the eukaryotic mevalonic acid (MVA) pathway or eukaryotic isopentenyl pyrophosphate isomerase (Idi) and isoprene synthase (IspS), were constructed and electroporated into
C. ljungdahlii
. These plasmids, one or two of which were introduced into the host cells, enabled the synthesis of mevalonate and of isoprene from fructose and from syngas (H
2
, CO
2
, and CO) and the conversion of mevalonate to isoprene. All of the heterologous enzymes of the MVA pathway, as well as Idi and IspS, were shown to be synthesized at high levels in
C. ljungdahlii
, as demonstrated by Western blotting, and were enzymatically active, as demonstrated by
in vivo
product synthesis. The quantities of mevalonate and isoprene produced here are far below what would be required of a commercial production strain. However, proposals are made that could enable a substantial increase in the mass yield of product formation.

IMPORTANCE
This study demonstrates the ability to synthesize a heterologous metabolic pathway in
C. ljungdahlii
, an organism capable of metabolizing either simple sugars or syngas or both together (mixotrophy). Syngas, an inexpensive source of carbon and reducing equivalents, is produced as a major component of some industrial waste gas, and it can be generated by gasification of cellulosic biowaste and of municipal solid waste. Its conversion to useful products therefore offers potential cost and environmental benefits. The ability of
C. ljungdahlii
to grow mixotrophically also enables the recapture, should there be sufficient reducing equivalents available, of the CO
2
released upon glycolysis, potentially increasing the mass yield of product formation. Isoprene is the simplest of the terpenoids, and so the demonstration of its production is a first step toward the synthesis of higher-value products of the terpenoid pathway.
High-Quality Whole-Genome Sequences of the Oligo-Mouse-Microbiota Bacterial Community
Authors: Garzetti Debora , Brugiroux Sandrine , Bunk Boyke , Pukall Rüdiger , McCoy Kathy D. , Macpherson Andrew J. , Stecher Bärbel 
Published: October 2017

ABSTRACT

The Oligo-Mouse-Microbiota (Oligo-MM
12
) is a community of 12 mouse intestinal bacteria to be used for microbiome research in gnotobiotic mice. We present here the high-quality whole genome sequences of the Oligo-MM
12
strains, which were obtained by combining the accuracy of the Illumina platforms with the long reads of the PacBio technology.
Draft Genome Sequence of an Atypical Highly Virulent Rabbit Staphylococcus aureus Strain
Authors: Német Zoltán , Albert Ervin , Nagy Tibor , Olasz Ferenc , Barta Endre , Kiss János , Dán Ádám , Bányai Krisztián , Hermans Katleen , Biksi Imre 
Published: October 2017

ABSTRACT

Rabbit staphylococcosis is one of the most important diseases in industrial rabbit production. We report here the draft genome sequence of
Staphylococcus aureus
strain 380/11, an atypical highly virulent (aHV) rabbit
Staphylococcus aureus
strain.
Whole-Genome Sequencing of a Human Clinical Isolate of the Novel Species
Klebsiella quasivariicola
sp. nov
Authors: Long S. Wesley , Linson Sarah E. , Ojeda Saavedra Matthew , Cantu Concepcion , Davis James J. , Brettin Thomas , Olsen Randall J. 
Published: October 2017

ABSTRACT

In a study of 1,777
Klebsiella
strains, we discovered KPN1705, which was distinct from all recognized
Klebsiella
spp. We closed the genome of strain KPN1705 using a hybrid of Illumina short-read and Oxford Nanopore long-read technologies. For this novel species, we propose the name
Klebsiella quasivariicola
sp. nov.
Complete Genome Sequence of Lactobacillus acidophilus Strain ATCC 53544
Authors: Dean Scott N. , Vora Gary J. , Walper Scott A. 
Published: October 2017

ABSTRACT

Here we present the complete genome sequence of
Lactobacillus acidophilus
ATCC 53544. The assembly contains 1,991,906 bp and is 99.7% similar to
L. acidophilus
NCFM. This strain was isolated from a rectal swab specimen of an infant and has previously been used as a feed supplement for animals.
Draft Genome Sequence of Halophilic
Hahella
sp. Strain CCB-MM4, Isolated from Matang Mangrove Forest in Perak, Malaysia
Authors: Sam Ka-Kei , Lau Nyok-Sean , Furusawa Go , Amirul Al-Ashraf Abdullah 
Published: October 2017

ABSTRACT

Hahella
sp. strain CCB-MM4 is a halophilic bacterium isolated from estuarine mangrove sediment. The genome sequence of
Hahella
sp. CCB-MM4 provides insights into exopolysaccharide biosynthesis and the lifestyle of the bacterium thriving in a saline mangrove environment.
Genome Sequences of Two NDM-1 Metallo-β-Lactamase-Producing Multidrug-Resistant Strains of Klebsiella pneumoniae with a High Degree of Similarity, One of Which Contains Prophage
Authors: Volozhantsev Nikolay V. , Kislichkina Angelina A. , Lev Anastasia I. , Mukhina Tatiana N. , Bogun Alexander A. , Ershova Olga N. , Alexandrova Irina A. , Fursova Nadezhda K. 
Published: October 2017

ABSTRACT

We report genome sequences of two NDM-1 metallo-β-lactamase-producing multidrug-resistant
Klebsiella pneumoniae
isolates of sequence type 147 (ST147) from one hospital. The genomes are highly similar and differ in prophage located in the chromosome of
K. pneumoniae
KPB-1470/16 and in the additional plasmid-carrying
bla
OXA-48
gene in
K. pneumoniae
KPB-417/16.
Modelling the process of falling behind and its psychophysiological consequences
Authors: Venhorst Andreas , Micklewright Dominic , Noakes Timothy D 
Published: October 2017

IntroductionA preceding article investigated the psychophysiological responses to falling behind a performance matched opponent. The following temporally linked cause–effect relationships were hypothesised: falling behind precedes deterioration in valence, deterioration in valence precedes development of an action crisis, experience of an action crisis precedes psychoneuroendocrinological distress response and non-adaptive distress response reduces conduciveness to high performance, thereby preceding performance decrement.MethodsIn this article, we applied structural equation modelling to test the extent to which the observed data fit the hypothesised cause–effect relationships. A five-step procedure was applied to model the interrelationships between the major study variables in the hypothesised temporal order.ResultsSignificant linear relationships were found between all hypothesised predictor and outcome variable pairs (p<0.024). The dynamic change in valence was a significant mediator (p=0.011) as it explained 35% of the relationship between falling behind and action crisis. All hypothesised cause–effect relationships continued to be significant after controlling for performance, descriptor, training and perceived strain variables. The observed data fitted the hypothesised structural model well with excellent model fit indices throughout.ConclusionWe applied, tested and confirmed the hypothesised debilitative psychophysiological processes that unfold in response to falling behind a performance matched opponent. The main findings were: deterioration in valence mediated the relationship between falling behind and action crisis, the mindset shift associated with an action crisis predicted increased blood cortisol concentrations and non-adaptive blood cortisol concentrations predicted performance decrement. The findings point towards the crucial role of affective and cognitive modifiers in centrally regulated and goal-directed exercise behaviour.
Epidemiology of imaging-detected tendon abnormalities in athletes participating in the Rio de Janeiro 2016 Summer Olympics
Authors: Jarraya Mohamed , Crema Michel D , Engebretsen Lars , Teytelboym Oleg M , Hayashi Daichi , Roemer Frank W , Skaf Abdalla Y , Guermazi Ali 
Published: October 2017

BackgroundTendon abnormalities are prevalent among both elite and non-elite athletes. Cross-sectional imaging modalities are used to confirm and evaluate the severity of such lesions.AimTo describe the demographics, prevalence, anatomical location and characteristics of tendon abnormalities in athletes who participated in the Rio de Janeiro 2016 Summer Olympic Games.MethodsWe recorded all sports injuries reported by the National Olympic Committee (NOC) medical teams and the Organizing Committee medical staff during the Rio 2016 Summer Olympics. Diagnostic imaging was performed through the official IOC clinic within the Olympic Village, using digital ultrasound machines and 3T and 1.5T MR scanners. Image interpretation was performed centrally by board-certified musculoskeletal radiologists with expertise in sports injuries.ResultsIn total, 11 274 athletes (5089 women (45%), 6185 men (55%)) from 207 NOCs were included. NOC and Rio de Janeiro 2016 medical staff reported 1101 injuries. Central review of radiological images revealed 156 tendon abnormalities in 109 athletes (51.2% male, mean age: 26.8, range 18–39). The supraspinatus tendon was the most commonly involved tendon (31 cases, 19.9%), followed by the Achilles tendon (20 cases, 12.8%) and patellar and infraspinatus tendons (12 cases, 7.7%). Tendon abnormalities were most commonly seen in track and field athletes (54 abnormalities, 34.6%).Conclusion156 tendon abnormalities were reported, most commonly in track and field athletes, and involving mainly the shoulder tendons, as well as Achilles and patellar tendons.
Improving the rates of electronic results acknowledgement at a tertiary eye care centre
Authors: Phua Val , Au Benjamin , Soh Yu Qiang , Husain Rahat 
Published: October 2017

BackgroundHundreds of thousands of tests are performed annually in hospitals worldwide. Safety Issues arise when abnormal results are not recognized promptly resulting in delayed treatment and increased morbidity and mortality. As a result Singapore’s largest healthcare group, Singhealth introduced an electronic result acknowledgement system. This system was adopted by the Singapore National Eye Centre (SNEC) in February 2016. Baseline measurements show that weekly numbers of unacknowledged results ranged from 193 to 617. The current standards of electronic results acknowledgement posts a significant patient safety hazard.MethodsRoot cause analysis was performed to identify contributory factors. Pareto principle was then used by the authors to identify the main contributory factors. We employed the rapid cycle improvement Plan-do-study-act (PDSA) strategy to test and evaluate implemented changes. Changes are implemented for 2 weeks and data collected prospectively. The data is analyzed the week after and the following PDSA actions are decided and instituted the following week. 3 PDSA cycles were undertaken in total.ResultsThe first PDSA cycle focused on raising awareness of the problem at hand, the number of unacknowledged results drastically decreased during the 1stweek of implementation of our PDSA from 617 to 254.The second PDSA cycle targeted the lack of knowledge of doctors involved in the electronic result acknowledgement process. There was a trend downwards near the end of the cycle which continued through the week after.The third PDSA cycle targeted individual doctors and provided individual remedial training. Second line doctors were also equipped to better handle abnormal results. There was significant improvement with the number of unacknowledged abnormal results dropping to <5 a week.ConclusionsMultiple factors were identified to contribute to the low compliance to electronic acknowledgement of results. The role doctors play in the issue at hand was paramount and required careful handling in a professional manner with multiple reminders and emphasis on the importance of acknowledging and acting on the results.A significant improvement in the rates of acknowledgement of abnormal results was demonstrated with clear benefits to patient safety. Interventions can be replicated when implementing similar systems to other areas of healthcare.
Indoor Tanning Dependence in Young Adult Women
Authors: Mays Darren , Atkins Michael B. , Ahn Jaeil , Tercyak Kenneth P. 
Published: October 2017

Abstract
Background: There is mounting evidence that young people can develop a dependence on indoor tanning, but research on factors associated with indoor tanning dependence remains limited.
Methods: This cross-sectional study investigated factors associated with indoor tanning dependence in a community sample of 389 non-Hispanic white young adult women ages 18 to 30 who had indoor tanned ≥1 time in the past year. Participants completed measures of indoor tanning dependence, including the modified CAGE and modified Diagnostic and Statistical Manual for Mental Disorders-IV psychiatric screening assessments, indoor tanning behavior and beliefs, and behavioral and psychiatric comorbidity.
Results: Overall, 22.6% of the sample screened positive for indoor tanning dependence. In multivariable analyses, indoor tanning dependence was associated with younger age of indoor tanning initiation [adjusted odds ratio (aOR) = 0.79; P = 0.017], indoor tanning ≥20 times in the past year (aOR = 3.03; P = 0.015), stronger beliefs about the benefits of tanning (aOR = 2.15; P = 0.004), greater perceived susceptibility to indoor tanning risks (aOR = 2.72; P &lt; 0.001), stronger beliefs about physical appearance (aOR = 1.73; P = 0.037), and depressive symptoms (aOR = 3.79; P &lt; 0.001).
Conclusions: Indoor tanning dependence among young, non-Hispanic white women is associated with behaviors that increase the risk of skin cancer, beliefs favoring the perceived benefits of tanning, and comorbid risks such as stronger beliefs about physical appearance and depressed mood.
Impact: Comprehensive skin cancer prevention efforts should address indoor tanning dependence among young women and its leading risk factors. Cancer Epidemiol Biomarkers Prev; 26(11); 1636–43. ©2017 AACR.
A Multicenter Phase I Study Evaluating Dual PI3K and BRAF Inhibition with PX-866 and Vemurafenib in Patients with Advanced BRAF V600–Mutant Solid Tumors
Authors: Yam Clinton , Xu Xiaowei , Davies Michael A. , Gimotty Phyllis A. , Morrissette Jennifer J.D. , Tetzlaff Michael T. , Wani Khalida M. , Liu Shujing , Deng Wanleng , Buckley Meghan , Zhao Jianhua , Amaravadi Ravi K. , Haas Naomi B. , Kudchadkar Ragini R. , Pavlick Anna C. , Sosman Jeffrey A. , Tawbi Hussein , Walker Luke , Schuchter Lynn M. , Karakousis Giorgos C. , Gangadhar Tara C. 
Published: October 2017

Abstract
Purpose: The objectives of the study were to evaluate the safety of daily oral PX-866 in combination with twice daily vemurafenib and to identify potential predictive biomarkers for this novel combination.
Experimental Design: We conducted a phase I, open-label, dose-escalation study in patients with advanced BRAF V600–mutant solid tumors. PX-866 was administered on a continuous schedule in combination with vemurafenib. Patients underwent a baseline and on-treatment biopsy after 1-week of PX-866 monotherapy for biomarker assessment.
Results: Twenty-four patients were enrolled. The most common treatment-related adverse events were gastrointestinal side effects. One dose-limiting toxicity (DLT) of grade 3 rash and one DLT of grade 3 pancreatitis were observed in cohort 2 (PX-866 6 mg daily; vemurafenib 960 mg twice daily) and cohort 3 (PX-866 8 mg daily; vemurafenib 960 mg twice daily), respectively. Of 23 response-evaluable patients, seven had confirmed partial responses (PR), 10 had stable disease, and six had disease progression. Decreases in intratumoral pAKT expression were observed following treatment with PX-866. Patients who achieved PRs had higher rates of PTEN loss by IHC (80% vs. 58%) and pathogenic PTEN mutations and/or deletions (57% vs. 25%). Two patients with durable PRs had an increase in intratumoral CD8+ T-cell infiltration following treatment with PX-866.
Conclusions: PX-866 was well tolerated at its maximum tolerated single-agent dose when given in combination with a modified dose of vemurafenib (720 mg twice daily). Response to treatment appeared to be associated with PTEN loss and treatment with PX-866 seemed to increase CD8+ T-cell infiltration in some patients. Clin Cancer Res; 24(1); 22–32. ©2017 AACR.
‘You are a Priest Forever!’ Jesus’ Indestructible Life in Hebrews 7:16
Authors: Kibbe Michael 
Published: October 2017

Abstract
Hebrews 7:16 suggests that Jesus entered the priesthood by virtue of his “indestructible life.” But what sort of life is that? How and when did he obtain it? If it pertains to his resurrected-human life, was he therefore not a priest prior to that moment (including, in particular, during his death on the cross)? If it pertains to his divine life (thus he possesses it always), in what sense could the man Jesus have actually died? I suggest that the author has neither deity nor resurrection in view as the source of Jesus’ indestructible life; rather, the point is merely that Jesus’ perpetual existence undergirds the oath that immediately follows in 7:17. However, Hebrews as a whole requires that we envision that existence in relation to both Jesus’ deity and his resurrection, and I argue, furthermore, that the two cohere via the divine Son’s agency in his own resurrection.
The Lord Will Reveal the Lord
Authors: Wenkel David H. 
Published: October 2017

Abstract
The first pastoral epistle to Timothy follows a relatively traditional Jewish doctrine in the context of second temple Judaism: God is one “whom no one has ever seen or can see” (1 Timothy 6:16). This parallels a statement in chapter one in which God is described as “invisible” (1 Timothy 1:17). In both statements, the invisibility of God is contrasted with the visible quality of Jesus’ spiritual power or his second appearing. This paper argues that the Jewish monotheistic doctrine of God has been developed in a proto-Trinitarian manner by utilizing the qualities of invisibility and visibility.
Long-Term Quality of Life After Extracorporeal Membrane Oxygenation in ARDS Survivors: Systematic Review and Meta-Analysis
Authors: Wilcox M. Elizabeth , Jaramillo-Rocha Valente , Hodgson Carol , Taglione Michael S. , Ferguson Niall D. , Fan Eddy 
Published: October 2017

Purpose: Extracorporeal membrane oxygenation (ECMO) is an increasingly prevalent treatment for acute respiratory failure (ARF). To evaluate the impact of ECMO support on long-term outcomes for critically ill adults with ARF. Methods: We searched electronic databases 1948 through to November 30 2016; selected controlled trials or observational studies of critically ill adults with acute respiratory distress syndrome, examining long-term morbidity specifically health-related quality of life (HRQL); 2 authors independently selected studies, extracted data, and assessed methodological quality. Analysis: Of the 633 citations, 1 randomized controlled trial and 5 observational studies met the selection criteria. Overall quality of observational studies was moderate to high (mean score on Newcastle-Ottawa scale, 7.2/9; range, 6-8). In 3 studies (n = 245), greater decrements in HRQL were seen for survivors of ECMO when compared to survivors of conventional mechanical ventilation (CMV) as measured by the Short Form 36 (SF-36) scores ([ECMO-CMV]: 5.40 [95% confidence interval, CI, 4.11 to 6.68]). As compared to CMV survivors, those who received ECMO experienced significantly less psychological morbidity (2 studies; n = 217 [ECMO-CMV]: mean weighted difference [MWD], −1.31 [95% CI, −1.98 to −0.64] for depression and MWD, −1.60 [95% CI, −1.80 to −1.39] for anxiety). Conclusions: Further studies are required to confirm findings and determine prognostic factors associated with more favorable outcomes in survivors of ECMO.
Antiphospholipid antibodies and non-thrombotic manifestations of systemic lupus erythematosus
Authors: İlgen U , Yayla M E , Ateş A , Okatan İE , Yurteri E U , Torgutalp M , Keleşoğlu A B D , Turgay T M , Kınıklı G 
Published: October 2017

Objectives The aim of this study was to investigate the association between antiphospholipid antibodies and non-thrombotic and non-gestational manifestations of systemic lupus erythematosus. Methods Systemic lupus erythematosus patients with persistently positive antiphospholipid antibodies or lupus anticoagulant were identified and grouped as systemic lupus erythematosus with antiphospholipid syndrome (SLE-APS), systemic lupus erythematosus with positive antiphospholipid antibodies/lupus anticoagulant without antiphospholipid syndrome (SLE-aPL), and systemic lupus erythematosus with negative aPLs (SLE-No aPL). Groups were compared in terms of non-thrombotic systemic lupus erythematosus manifestations and laboratory features retrospectively. Results A total of 150 systemic lupus erythematosus patients, 26 with SLE-APS, 25 with SLE-aPL, and 99 with SLE-No aPL, were identified. Livedo reticularis, neurologic involvement, and thrombocytopenia were more common in antiphospholipid antibody positive systemic lupus erythematosus cases. Malar rash, arthritis, and pleuritis were more common in the SLE-No aPL, SLE-APS, and SLE-aPL groups, respectively. Positivity rates and titers of specific antiphospholipid antibodies did not differ between the SLE-APS and SLE-aPL groups. Conclusions Presence of antiphospholipid syndrome or persistent antiphospholipid antibodies may be related to non-thrombotic and non-gestational systemic lupus erythematosus manifestations. Patients with systemic lupus erythematosus plus antiphospholipid syndrome and persistent antiphospholipid antibodies without antiphospholipid syndrome also differ in terms of systemic lupus erythematosus manifestations.