bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium
Authors: Mumpuni Aris , Ekowati Nuraeni , Purnomowati Purnomowati , Purwati Endang Sri 
Published: December 2017

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Pleurotus ostreatus</span></em><span lang="EN-GB"> cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of </span><em><span lang="EN-GB">P. ostreatus</span></em><span lang="EN-GB"> on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating </span><em><span lang="EN-GB">P. ostreatus</span></em><span lang="EN-GB"> in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.</span></p>
Expression of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B in Escherichia coli and Its Recovery from Inclusion Body
Authors: Savalas Lalu Rudyat Telly , Sedijani Prapti , Hadisaputra Saprizal , Ardhuha Jannatin , Lestari Chomsa Asih , Wahidah Etty Nurul 
Published: December 2017

<p class="IsiAbstrakIndo"><span lang="EN-GB">The present study aims at expressing and partially purifying PtpB in active form. To achieve this, Mtb PtpB gene has been cloned into pET30a vector and overexpressed in Escherichia coli BL 21(DE3) under IPTG induction in the form of an inclusion body. Following resolubilization by urea and dialysis, the resulted PtpB has been shown to be active against para-Nitrophenyl phosphate. It is concluded that the resulted PtpB has had been recovered from inclusion body to give the active form of the enzyme, and thus the success in overexpressing PtpB provides the required material to investigate the biochemical properties of the pathogen virulence factor further. </span></p>
Genetic Variability of Local Corn Cultivars from Kisar Island Southwest Maluku Regency using Microsatellite Molecular Marker
Authors: Sinay Hermalina , Karuwal Ritha Lusian 
Published: December 2017

<p class="IsiAbstrakIndo"><span lang="EN-GB">This research aim was to reveal the genetic variability of local corn cultivars from Kisar Island using microsatellite molecular marker. The use of microsatellite markers is based on one reasons that the genetic information is polymorphic, and will be better to identify genetic diversity of plants including corn. Corn cultivars used were obtained from farmer in Kisar Island, and three reference varieties as outgroup were obtained from Institute of Cereals in Maros South Sulawesi. Polymerase chain reaction was conducted following the protocols from Fast Start kit (Qiagen-USA). The data obtained then analyzed with MVSP 3.1A Software for dendogram construction. The results showed that the appearance of DNA bands was vary (monomorphic and polymorphic) for local cultivars as well as reference varieties, with one or two bands on each primer. Based on the dendogram, there are three main clusters with similarity index ranged from 20% -100%. It can be concluded that there is very low similarity and distant kinship of local cultivars. The novelty of this research is knowing kinship relationship between local corn in Kisar Island which is not known yet. This results is expected to provide benefits regarding the breeding program, and for instance, it can be an important information regarding the development of local corn as a source in assembly the superior corn cultivars. </span></p>
Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture
Authors: Manuhara Yosephine Sri Wulan , Kusuma Dannis Yuda , Sari Rafika Lailiyatul Kurnia , Kristanti Alfinda Novi 
Published: December 2017

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Gynura procumbens</span></em><span lang="EN-GB"> has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of </span><em><span lang="EN-GB">G. procumbens</span></em><span lang="EN-GB"> in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of </span><em><span lang="EN-GB">G. procumbens</span></em><span lang="EN-GB"> adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance. </span></p>
Caída y descomposición de hojarasca en los bosques ribereños del manantial de Cañaverales, Guajira, Colombia
Authors: Fuentes Molina Natalia , Rodriguez Barrios Javier , Isenia Leon Samir 
Published: December 2017

Se midieron los flujos de materia orgánica aportados por la hojarasca al manantial de Cañaverales (La Guajira, Colombia), durante un período de seis meses (seco y lluvioso). La caída mensual fue de 0,17 t·ha-1·mes-1, que extrapolada a un año representó 2,08 t·ha-1·año-1, valor intermedio a los registrados en otros bosques secos tropicales. Se evidenció una correlación significativa entre la caída de hojarasca y la precipitación. Los aportes orgánicos fueron dominados por la fracción foliar (66,61 %), seguidos del material reproductivo (15,92 %), el material leñoso (11,12 %) y misceláneo (6,36 %). La mayor caída de hojarasca foliar se regis­tró para Prosopis juliflora (1,31 t·ha-1·año-1) y la menor caída la presentó Brosimum alicastrum (0,46 t·ha-1·año-1). Las especies P. juliflora y Anacardium excelsum presentaron los mayores aportes de hojarasca foliar, lo que sugiere que podrían ser especies potenciales para su inclusión en actividades de restauración de zonas con corrientes de agua. P. juliflora fue la especie que presentó mayores tasas de descomposición de hojarasca (0,242 año-1), mientras que la Sterculia apetala fue la más persistente (0,079 año-1). Los resultados indican que la caída de hojarasca está condicionada por la composición del bosque y las variaciones climáticas.
Integración, carga viral y niveles de ARN mensajero de E2 de VPH 16 en la progresión de lesiones intraepiteliales cervicales
Authors: Trujillo Esperanza , Sanchéz Ricardo , Bravo María Mercedes 
Published: December 2017

Entre las lesiones intraepiteliales escamosas cervicales (LIE) es importante distinguir aquellas asociadas con mayor riesgo de cáncer de cuello uterino. El objetivo de este trabajo fue evaluar si los niveles de expresión de E2 del VPH16 en mujeres con LIE y con evidencia de integración viral se asocian con el grado de la lesión. Se analizaron 109 cepillados cervicales positivos para VPH 16 provenientes de 19 mujeres sin LIE, 45 mujeres con LIE de bajo grado (LIEBG) y 45 mujeres con LIE de alto grado (LIEAG). Se cuantificó el número de copias de ARNm de E2 y de los genes E2 y E6 mediante PCR en tiempo real para determinar la carga viral (E6) y la proporción E2/E6 para evaluar la integración viral. Se encontraron frecuencias similares de expresión de E2 en LEIBG y LEIAG 15/45 (33 %), la frecuencia en mujeres sin lesión fue menor 3/19 (15,8 %), todos los casos en los que se observó expresión del gen E2 tenían mezcla de ADN viral episomal e integrado. La carga viral aumentó significativamente a mayor grado de la lesión (p=0,049), mientras que la proporción E2/E6 disminuyó (p=0,049). El análisis ROC mostró una baja capacidad de los tres parámetros virales para distinguir entre lesiones de bajo y alto grado. En conclusión, aunque las lesiones con presencia de ADN viral mixto e integrado y expresión de E2 podrían estar en menor riesgo de progresión, y la carga viral y la integración se relacionaron con mayor gravedad de la lesión, su valor clínico como biomarcadores de LEIAG es limitado.
Effect of cutting and storage temperature on Packham’s Triumph pears (Pyrus communis L.)
Authors: Obando Ulloa Javier Mauricio , Escalona Contreras Víctor Hugo 
Published: December 2017

En los frutos mínimamente procesados en fresco (MPF) el tipo de corte y la temperatura son factores por considerar para mantener una calidad y apariencia adecuada. El objetivo de este trabajo fue evaluar los efectos del corte y la temperatura de conservación sobre la actividad metabólica, color, firmeza, perfil aromático y concentración de ácidos orgánicos y azúcares de peras (Pyrus communis L. cv. Packham’s Triumph). Los frutos enteros fueron lavados, pelados, cortados en cascos o cubos, envasados en tarrinas y posteriormente almacenados 8 d a 5 o 8 ºC. El tipo de corte y la temperatura tuvieron un efecto significativo sobre los parámetros fisiológicos, color, firmeza y en el contenido de ácidos orgánicos y azúcares. Sin embargo, el contenido de compuestos aromáticos volátiles se redujo independientemente del tipo de corte y las temperaturas de conservación. Por tanto, esta variedad de peras Packham’s Triumph deben ser procesadas en cascos y deben almacenarse a 5º C para mantener una calidad general óptima durante 8 días.
Biomass decomposition dynamic in agroforestry systems with Theobroma cacao L. in Rionegro, Santander (Colombia)
Authors: Rojas M. Jairo , Caicedo Victor , Jaimes Yeirme 
Published: December 2017

The study was carried out in an agroforestry system (AFS) with cacao trees already established in La Suiza Research Center, Corpoica, located in Rionegro, Santander (Colombia). The objective was to evaluate biomass input and nutrient release rate of the species that comprise the AFS (Gmelina arbórea, Gliricidia sepium, Cedrela odorata, Theobroma cacao). The plant material decomposition process of the species was monitored with decomposition bags after 8, 15, 23, 84 and 113 days, evaluating remnant weight and nitrogen, phosphorous, potassium, calcium and magnesium content. Results showed significant differences in plant material decomposition of the species considered. The largest weight loss was found in G. arborea (87.55%) and the lowest in C. odorata (40.01%). The highest nutrient release value was found in G. arborea followed by G. sepium, and the lowest in T. cacao and C. odorata.Therefore, depending on the species that comprise the AFS there is a differential leaf biomass decomposition dynamic and hence, of the nutrient input to the soil. Of the species evaluated the highest input of new organic matter to the soil in this AFS comes from G. arborea. Altogether, the litter generated by the species evaluated contributed with 10% of the nutrients required for a cacao harvest.
Leaf area index of potato (Solanum tuberosum L.) crop under three nitrogen fertilization treatments
Authors: Villa Pedro Manuel , Sarmiento Lina , Rada Fermín José , Machado Daniel , Rodrigues Alice Cristina 
Published: December 2017

Nitrogen fertilization has considerable effects on growth and yield of potato crop. Leaf expansion is one of the most important growth parameters that can be used to optimize nitrogen fertilization management in agroecological practices. We evaluated the effect of nitrogen fertilization on the leaf area index (LAI) of potato crop during different phenological stages by two different methods. Experimental plots were established in a randomized block design with the following treatments: no nitrogen supply (0-N), 133 kg N ha-1 (133-N), and 400 kg N ha-1 (400-N), using the Granola variety, at the Andes region, Merida city, Venezuela. We measured leaf area at the main phenological stages of the crop using a plant canopy analyzer. LAI showed significant differences among treatments and phenological stages, except during the emergence stage. The maximum LAI values were obtained 55 days after emergence: 400-N (1.42±0.16) > 133-N (0.92±0.21) > 0-N (0.34±0.08). Nitrogen fertilization had a significant effect on the development of LAI in potato crop along its different phenological stages.
Evaluation of the growth and quality of lettuce (Lactuca sativa L.) in a closed recirculating hydroponic system
Authors: Fraile-Robayo Rafael David , Álvarez-Herrera Javier Giovanni , Reyes M. Andrea Johana , Álvarez-Herrera Omar Ferney , Fraile-Robayo Ana Lucía 
Published: December 2017

The production of lettuce in hydroponic systems with a recirculating nutrient solution has been growing, so it is necessary to evaluate the growth and quality of production under this system. Two harvest cycles were evaluated, comparing the behavior of physiological variables and growth rates on lettuce plants in a hydroponic system with a plastic cover. Lettuce plants were planted at 30 days after germination in an NFT hydroponic system. Nutrient solutions were prepared with sources of potassium nitrate, calcium nitrate, urea phosphate, magnesium sulfate and a source of minor nutrifeed. The second cycle had the highest total dry mass and leaf area index (LAI) at 43 days after transplant (dat). The relative growth rate (RGR) declined over time. The absolute growth rate (AGR) presented a sigmoid behavior as a gaussian bell shape; the leaf area index (LAI) increased until 43 dat, with the second cycle presenting the highest value; the net assimilation rate (NAR) decreased over time, with the second cycle having the highest value at 22 dat. The chlorophyll content for this variety was low, with a yellow pigmentation in the plant. The stomatal conductance (SC) in the two cycles at transplant time presented low values caused by the stress leaded by an imbalance in the pH of the solution, when the plants adapted to the system, this value increased.
Congenital laryngeal saccular cyst: a case report and literature review
Authors: Anzola Diana Patricia , Guerra Ricardo Enrrique , Puerta Mauricio 
Published: December 2017

Introducción. El quiste sacular congénito es una lesión rara, pero benigna, dada por la dilatación del sáculo laríngeo que se llena de moco y que no se comunica con el lumen laríngeo. Según la literatura, su tratamiento definitivo es quirúrgico. Objetivo. Realizar una revisión de la literatura y el reporte de un caso de quiste sacular laríngeo congénito y su tratamiento mediante abordaje endoscópico y uso de radiofrecuencia.Materiales y métodos. Se realizó la presentación del caso clínico y se llevó a cabo una revisión de la literatura en las bases de datos de Pubmed y Tripdatabase usando palabras claves descritas.Resultados. Esta patología es escasa, con una incidencia de 1.82 casos por 100 000 nacidos vivos. El diagnostico se realiza por medio de endoscopia laríngea, imágenes o revisión de la clínica. El caso expuesto es de una paciente recién nacida que presenta estridor y dificultad respiratoria y es diagnosticada con quiste sacular laríngeo, el cual es resecado de forma quirúrgica por medio de endoscopia con uso de radiofrecuencia. El procedimiento da como resultado la no reaparición del quiste.Conclusiones. Pese a no existir muchos reportes de caso, hay que conocer las características del quiste sacular congénito para poder realizar el tratamiento adecuado con los recursos disponibles. Es posible realizar resección endoscópica de esta lesión si es <3cm por medio de radiofrecuencia, un método seguro y eficaz.
Ascending aortic disease in a patient with Marfan Syndrome. Case report
Authors: Serrrano Olmedo Stefania Del Cisne , Espinoza Saquicela Edison Ricardo 
Published: December 2017

La incidencia de síndrome de Marfan que ha sido reportada a nivel mundial es de 1 en 5000 casos, de los cuales aproximadamente el 80% o más desarrollan complicaciones cardiovasculares. La disección aórtica torácica aguda requiere una rotura en la íntima de la aorta, que forma parte del síndrome aórtico agudo. En la disección, la sangre pasa a través de la rotura y separa la íntima de la media o la adventicia, lo que crea una falsa luz intravascular. Un diagnóstico temprano incide directamente en las posibilidades de supervivencia, pues se trata de una emergencia médica que puede llevar a la muerte, incluso con un tratamiento óptimo. Se presenta el caso de un hombre de 26 años con antecedente de síndrome de Marfan, dolor retro esternal lancinante, náusea, vómito, disnea de medianos esfuerzos que evolucionó a ortopnea, cianosis peribucal, soplo de insuficiencia aórtica y soplo sistólico mitral.Se realizaron estudios complementarios (radiografía de tórax, electrocardiograma, angiografía, tomografía, ecocardiograma) y se le diagnosticó disección de aorta ascendente tipo A Stanford, por lo que se decidió iniciar tratamiento quirúrgico mediante reemplazo de la raíz aórtica mediante prótesis compuesta y reconstrucción coronaria tipo Bentall y de Bono. En el procedimiento se presentó desestructuración de botón coronario derecho, por lo que fue necesario realizar puente venoso con hemoducto venoso de safena interno izquierdo. Se logró destete de circulación extra corpórea y luego se observó bajo gasto de carácter refractario (pese a soporte vasopresor a dosis máxima), fibrilación ventricular refractaria y asistolia; el paciente no mostró recuperación y falleció como consecuencia de un infarto agudo de miocardio transoperatorio. El tratamiento de la disección aórtica de aorta ascendente sigue siendo un desafío terapéutico. El diagnóstico oportuno tiene relación directa con la esperanza de vida de quienes lo padecen, de ahí su importancia diagnóstica y su manejo.
Delusional infestation. Ekbom´s syndrome in a 47-year-old woman. Case report
Authors: Olivera Mario Javier , Porras Villamil Julián Felipe , López Moreno Gabriela Andrea , Toquica Gahona Christian Camilo , Paez Ardila Hugo , Maldonado Lara Eliana 
Published: December 2017

Introduction: Delusional infestation is a rare psychiatric disorder defined as a condition in which the patient has the unshakable belief and perception of being infested with parasites. Its treatment is difficult, and frequently includes antipsychotic medications (such as olanzapine or aripiprazole). Non-pharmacological treatment, particularly psychotherapy, can be used for less severe cases. Dermatologists and psychiatrists must take a multi-disciplinary approach (preferably in a psycodermatology dedicated clinic) since this type of patients sometimes refuse treatment.Case description: A 53-year-old female businesswoman describes a clinical history of five years of visual hallucinations, depressive symptoms, and generalized pruritus, along with the use of toxic substances to “clean” her skin and cloths. She reports similar symptoms in some relatives but they were not evaluated. Blood tests and analyses of the “specimen” brought by the patient were performed, yielding negative results. The patient had never been assessed by any specialist, and showed disoriented during the consultation. Follow-up was not possible due to the reluctance of the patient to follow the indications and seek psychiatric treatment. Moreover, the patient did not respond to further communication attempts.Discussion: Delusional infestation is an uncommon disease that endangers the patients and the people around them. Its treatment is difficult and long, and not conducting proper follow-up is a great risk. Its prevalence and incidence is variable and generally unknown. It can affect the patient, their next of kin, pets or the environment, and the “pathogen” can be a living organism or an inanimate object. Conclusion: This case is important as it shows the hardships of treatment, adequate follow-up and care, as well as the need to improve how these patients are approached. Additionally, both classical and uncommon signs and symptoms could be observed as the patient stated that her relatives were affected (possible delusional infestation by proxy).
De culpable a perseguido: Baudelaire y el proceso de Las Flores del Mal
Authors: Zapata Juan 
Published: December 2017

¿Cómo el poeta de Las Flores del Mal pasó de ser un escritor proscrito, y en consecuencia sospechoso, al autor perseguido e incomprendido que ha inmortalizado la historia literaria? ¿Cómo Baudelaire pudo desviar a su favor una circunstancia que, a primera vista, era desafortunada? Baudelaire supo utilizar el escándalo que provocó la publicación de Las Flores del Mal para promover su figura pública. Un análisis de las posturas discursivas desplegadas por Baudelaire antes, durante y después del proceso nos permitirá comprender mejor la inversión que el poeta hace de su condenación, al transformar la sanción penal y moral, así como la exclusión a la que esta conduce, en un signo de su grandeza y de su gloria póstuma.