bluuebunny/crossref_metadata_2025_split huggingface dataset:
The lncRNA CADM2-AS1 promotes gastric cancer metastasis by binding with miR-5047 and activating NOTCH4 translation
Authors: Zhang Yu-Tong , Zhao Li-Juan , Zhou Teng , Zhao Jin-Yuan , Geng Yin-Ping , Zhang Qiu-Rong , Sun Pei-Chun , Chen Wen-Chao 
Published: September 2024

BackgroundMulti-organ metastasis has been the main cause of death in patients with Gastric cancer (GC). The prognosis for patients with metastasized GC is still very poor. Long noncoding RNAs (lncRNAs) always been reported to be closely related to cancer metastasis.MethodsIn this paper, the aberrantly expressed lncRNA CADM2-AS1 was identified by lncRNA-sequencing in clinical lymph node metastatic GC tissues. Besides, the role of lncRNA CADM2-AS1 in cancer metastasis was detected by Transwell, Wound healing, Western Blot or other assays in vitro and in vivo. Further mechanism study was performed by RNA FISH, Dual-luciferase reporter assay and RT-qPCR. Finally, the relationship among lncRNA CADM2-AS1, miR-5047 and NOTCH4 in patient tissues was detected by RT-qPCR.ResultsIn this paper, the aberrantly expressed lncRNA CADM2-AS1 was identified by lncRNA-sequencing in clinical lymph node metastatic GC tissues. Besides, the role of lncRNA CADM2-AS1 in cancer metastasis was detected in vitro and in vivo. The results shown that overexpression of the lncRNA CADM2-AS1 promoted GC metastasis, while knockdown inhibited it. Further mechanism study proved that lncRNA CADM2-AS1 could sponge and silence miR-5047, which targeting mRNA was NOTCH4. Elevated expression of lncRNA CADM2-AS1 facilitate GC metastasis by up-regulating NOTCH4 mRNA level consequently. What’s more, the relationship among lncRNA CADM2-AS1, miR-5047 and NOTCH4 was further detected and verified in metastatic GC patient tissues.ConclusionsLncRNA CADM2-AS1 promoted metastasis in GC by targeting the miR-5047/NOTCH4 signaling axis, which may be a potential target for GC metastasis.
Dissecting the causal relationship between moderate to vigorous physical activity levels and cognitive performance: a bidirectional two-sample Mendelian randomization study
Authors: Fang Qi , Zhang Jinmin 
Published: September 2024

IntroductionRecent studies increasingly suggest that moderate-to-vigorous physical activity (MVPA) impacts cognitive risk. However, the bidirectional nature of this relationship warrants further exploration. To address this, we employed a Mendelian randomization (MR) approach, analyzing two distinct samples.MethodsThese analyses utilized published genome-wide association study (GWAS) summary statistics for MVPA (n = 377,234) and cognitive performance (n = 257,841). Our primary method was the inverse variance weighted (IVW) model with random effects, aiming to deduce potential causal links. Additionally, we employed supplementary methods, including MR Egger regression, Weighted median, Weighted mode, and Simple mode. For sensitivity analysis, tools like the MR Egger test, Cochran’s Q, MR PRESSO, and leave-one-out (LOO) were utilized.ResultsOur findings indicate a decrease in cognitive risk with increased MVPA (Odds Ratio [OR] = 0.577, 95% Confidence Interval [CI]: 0.460–0.723, p = 1.930 × 10–6). Furthermore, enhanced cognitive levels corresponded to a reduced risk of inadequate MVPA (OR = 0.866, 95% CI: 0.839–0.895, p = 1.200 × 10–18).DiscussionIn summary, our study demonstrates that MVPA lowers cognitive risk, while poor cognitive health may impede participation in MVPA. Overall, these findings provide valuable insights for developing personalized prevention and intervention strategies in health and sports sciences.
Evaluating the psychometric properties of three WHO instruments to assess knowledge about human rights, attitudes toward persons with mental health conditions and psychosocial disabilities, and practices related to substitute decision-making and coercion in mental health
Authors: Moro Maria Francesca , Gyimah Leveana , Susser Ezra , Ansong Joana , Kane Jeremy , Amissah Caroline , Gureje Oye , Osei Akwasi , Norcini Pala Andrea , Taylor Dan , Drew Nathalie , Kofie Humphrey , Baingana Florence , Ohene Sally-ann , Addico Nii Lartey , Fatawu Abdul , Atzeni Michela , D’Oca Silvia , Carta Mauro Giovanni , Funk Michelle 
Published: September 2024

BackgroundInstruments to assess the knowledge about the rights of persons with mental health conditions and psychosocial disabilities, the attitudes toward their role as rights holders, and mental health professionals’ practices related to substitute decision-making and coercion are either missing or lack evaluation of their validity and reliability.AimThe aim of this study is to evaluate the validity and reliability of three instruments developed to fill this gap in the literature, the World Health Organization’s QualityRights (WHO QR) Knowledge questionnaire, the WHO QR Attitudes questionnaire, and the WHO QR Practices questionnaire.MethodsA sample of participants was recruited and completed an online survey. Content validity and face validity were assessed for the three questionnaires. Based on the characteristics of the questionnaires, different approaches were used to assess their construct validity (confirmatory factor analysis, known group validity, and convergent and divergent validity). Internal consistency was evaluated using Cronbach’s alpha and test re-test reliability using Pearson’s and Spearman’s r coefficients.ResultsThe analyses conducted indicate that the three questionnaires are valid and reliable instruments to evaluate the knowledge about the rights of persons with mental health conditions and psychosocial disabilities, the attitudes toward their role as rights holders, and mental health professionals’ practices related to substitute decision-making and coercion.ConclusionThis finding lends support to the use of these instruments both within mental health services and in the general population for a better understanding of current knowledge, attitudes, and practices related to a human rights–based approach to mental health in mental health services and the community.
Decreased consumption of natural rewards in rhesus monkeys with prolonged methamphetamine abstinence
Authors: Zhou Jiahui , Su Hang , Tang Chengjie , Wu Xiaotian , Wang Zijing , Zhang Wenlei , Zhai Rongwei , Jiang Haifeng 
Published: September 2024

RationaleRelapse to drug use is a major clinical challenge in the treatment of addictive disorders, including psychostimulant use and may be exacerbated by reduced sensitivity to natural, non-drug reward. Given the relatively limited set of outcomes, and short withdrawal time in rodent studies, we conducted a more detailed assessment of the response to natural rewards in methamphetamine (METH) naive versus exposed monkeys during long-term abstinence.MethodsThis study introduced an improved sucrose preference test (iSPT) to assess natural reward seeking and consumption in monkeys with long-term abstinence after methamphetamine (METH) use. The test was administered to sixteen naive monkeys and five METH exposed monkeys that had been abstinent for at least 3 months.ResultsMETH exposed monkeys showed a lower sucrose preference score in both the iSPT (z = -2.10, p = 0.036) and the sucrose preference test (z = -2.61, p = 0.009). The sucrose preference score was significantly correlated with the latency of the establishment of stable sucrose-preference (r = -0.76, df = 46, p &lt; 0.001) but not with the other variables. Furthermore, water-sucrose switch latency and switch times were significantly negatively correlated (r = -0.50, df = 20, p = 0.02).ConclusionThese results show reductions in natural reward consumption during long-term methamphetamine abstinence.
Antibiotic-resistant bacteria and resistance-genes in drinking water source in north Shoa zone, Amhara region, Ethiopia
Authors: Abebe Tsegahun Asfaw , Gebreyes Demissew Shenkute , Abebe Bizuneh Asfaw , Yitayew Berhanu 
Published: September 2024

BackgroundThe growing number of antimicrobial-resistant bacteria in a range of environments poses a serious challenge to infectious disease prevention. Good water quality is critical to human health and has a direct impact on a country’s socio-economic growth. Therefore, assessing the bacteriological quality of drinking water provides benchmark data and provides insight into the development of further protection and treatment measures.MethodsA cross-sectional study was conducted from February 1, 2022, to September 31, 2023, in the diarrhea hotspot areas of North Shewa Zone (Minjar-Shenkora and Mojana-Wedera districts). Water samples were collected from drinking water sources (hand-pumps, boreholes, wells, spring water and ponds) to assess the quality following WHO guidelines. The collected water samples were processed for bacterial isolation, antimicrobial susceptibility testing, and detection of antimicrobial resistance genes. Data were entered and analyzed using the Statistical Package for the Social Sciences (SPSS) version 25.ResultsA total of (49/138, 35.5%) bacteria were isolated from 138 drinking water samples, with a positive rate of (41/138, 29.7%). Among the isolates, (16/138, 11.6%) were Staphylococcus aureus while (33/138, 23.9%) were members of Enterobacteriaceae. Relatively high resistance rate among all isolates were observed for the most prescribed antibiotics in Ethiopia, including erythromycin, cotrimoxazole, doxycycline, ceftriaxone, gentamicin, and chloramphenicol. However, a low resistance was observed for early introduced antibiotics such as ciprofloxacin and recently introduced antibiotics such as cefotaxime, ceftazidime, imipenem, and meropenem. Among the 49 bacteria isolates, (32/49, 65.3%) were multidrug-resistant (MDR) pathogens while (12/49, 24.5%) were ESβL producers. Different ESβL genes were detected in most bacterial isolates. The predominant ESβL genes were blaCTX-M-gp8/25 (6/33, 18.2%), blaCTX-M-gp9 (5/33, 15.2%), and blaCTX-M-gp1 (5/33, 15.2%).ConclusionThe result of this study suggests that most water sources in the study area were contaminated by various bacterial species that are resistant to different antibiotics. Various ESβL resistance genes have also been detected. Therefore, regular sanitary inspection and bacteriological analysis should be mandatory to protect drinking water sources from contamination and the persistence of resistant bacteria.
Pregnancy and associated factors among teenage females in Hula District, Sidama region, Ethiopia: a community-based cross-sectional study
Authors: Amenu Mekdes , Tediso Dansamo , Feleke Tihun , Fantahun Selam , Woldesemayat Endrias Markos 
Published: September 2024

BackgroundTeenage pregnancy causes serious health, social, and economic consequences, with 95% occurring in developing countries. A significant number of girls start childbearing at an early age in Ethiopia, which contributes to high infant and maternal morbidity and mortality. However, the information on teenage pregnancy and related variables is limited in the study area.ObjectiveTo assess the prevalence of teenage pregnancy and its associated factors among teenage females in the Hula District, Sidama, Ethiopia.MethodsA community-based cross-sectional study was employed among 518 teenagers, randomly selected between 15 February and 15 March 2023. An interviewer-administered questionnaire was used for data collection. Bivariate and multivariate logistic regression analyses were applied to assess the relationship under study.ResultsLiving in rural areas compared to urban [adjusted odds ratio (AOR) = 3.90; 95% confidence interval (CI): 1.30–11.3], lack of awareness about family planning methods (AOR = 5.90; 95% CI: 1.60–22.24), unfamiliarity with the availability of family planning services (AOR = 3.20; 95% CI: 1.08–9.24), and inadequate communication about sexual issues with parents (AOR = 3.61; 95% CI: 1.14–11.56) were independently associated with teenage pregnancy.ConclusionThe prevalence of teenage pregnancies in the Hula District was high. Factors such as residing in rural areas, limited access to information on family planning methods and services, as well as a lack of open discussions about sexual health were associated with teenage pregnancy.
An analytical study on changes in university students' attitudes toward physical activity
Authors: Nie JingJing 
Published: September 2024

PurposePositive attitudes toward physical activity have been consistently linked to health Behaviors. This study aims to validate the effectiveness of attitude change in the domain of exercise and identify the mechanisms through which this change occurs in the context of sports and promote exercise behavior.Methods103 university students (20.7 ± 1.97 years, 40 males, and 63 females) completed the implicit and explicit attitude tests. Based on their exercise levels assessed using the Physical Activity Rating Scale (PARS-3), participants were categorized into low and high exercise level groups. From this sample, 66 university students (19 males and 47 females) with low exercise levels completed a self-generated information task. Subsequently, physical activity was assessed through a skipping rope task.ResultsThe implementation of the self-generated information task resulted in notable alterations in implicit attitudes (ηi2 = 0.07) toward physical activity during subsequent Implicit Attitude Test (IAT) evaluations. Additionally, significant changes were observed in explicit attitudes (ηe2 = 0.12) toward physical activity among university students. Moreover, the use of self-generated information positively influenced immediate exercise behavior.DiscussionThis study provides valuable insights into the impact of attitudes on behaviors among university students. It suggests that attitudes toward physical activity can be effectively changed through the use of self-generated information methodology, leading to an enhancement in immediate exercise behavior. Such attitude change has the potential to promote healthier behaviors and reduce the risk of diseases. These findings hold implications for interventions aimed at enhancing physical activity levels among university students.
How physical exercise with others and prioritizing positivity contribute to (work) wellbeing: a cross-sectional and diary multilevel study
Authors: Gil-Beltrán Ester , Coo Cristian , Meneghel Isabella , Llorens Susana , Salanova Marisa 
Published: September 2024

IntroductionThis work is a dual study employing a cross-sectional approach and a diary method to investigate how physical exercise can become a habit. Guided by the Upward Spiral Theory of Lifestyle Change, we examined the role of prioritizing positivity and engaging in physical exercise with others as advantageous resources and their impact on the relational loop of physical exercise behavior, emotions, and engagement.MethodsThe first study involved a sample of 553 participants, and the second study included 146 participants, all of whom were employed and regularly engaged in physical exercise. We utilized structural equation modeling and multilevel analysis for the respective studies.ResultsThe results of the first study indicate that individuals exercise more when they experience higher levels of engagement and positive emotions, particularly when exercising with others and prioritizing positivity. The findings of the second study reveal that prioritizing positivity acts as a precursor to positive emotions during physical exercise, which in turn reinforces the relational loop between emotions and exercise behavior.DiscussionBoth studies conclude that individuals who prioritize positivity experience better psychological wellbeing and higher engagement in physical exercise.
Nested-PCR vs. RT-qPCR: A Sensitivity Comparison in the Detection of Genetic Alterations in Patients with Acute Leukemias
Authors: Pessoa Flávia Melo Cunha de Pinho , de Oliveira Marcelo Braga , Barreto Igor Valentim , Machado Anna Karolyna da Costa , Oliveira Deivide Sousa de , Ribeiro Rodrigo Monteiro , Medeiros Jaira Costa , Maciel Aurélia da Rocha , Silva Fabiana Aguiar Carneiro , Gurgel Lívia Andrade , de Albuquerque Kaira Mara Cordeiro , Lopes Germison Silva , Vieira Ricardo Parente Garcia , Arraes Jussara Alencar , Alencar Filho Meton Soares de , Khayat André Salim , Moraes Maria Elisabete Amaral de , de Moraes Filho Manoel Odorico , Moreira-Nunes Caroline Aquino 
Published: September 2024

The detection of genetic alterations in patients with acute leukemias is essential for the targeting of more specific and effective therapies. Therefore, the aim of this study was to compare the sensitivity of Nested-PCR and RT-qPCR techniques in the detection of genetic alterations in patients with acute leukemias. This study included samples from 117 patients treated at the Fortaleza General Hospital. All samples were submitted to analysis using the Nested-PCR and the RT-qPCR techniques. Acute Myeloid Leukemia (AML) patients’ samples were submitted to the analysis of the following alterations: FLT3-ITD, RUNX1::RUNX1T1, CBFB::MYH11 and PML::RARA; meanwhile, BCR::ABL1, TCF3::PBX1, KMT2A::AFF1, ETV6::RUNX1, and STIL::TAL1 fusions were investigated in the Acute Lymphoblastic Leukemia (ALL) patients’ samples. Throughout the study, 77 patients were diagnosed with AML and 40 with ALL. Among the 77 AML patients, FLT3-ITD, RUNX1::RUNX1T1, PML::RARA, and CBFB::MYH11 were detected in 4, 7, 10 and 8 patients, respectively. Among the 40 ALL patients, the presence of 23 patients with BCR::ABL1 translocation and 9 patients with TCF3::PBX1 translocation was observed through the RT-qPCR methodology. Overall, the present study demonstrated that the RT-qPCR technique presented a higher sensitivity when compared to the Nested-PCR technique at the time of diagnosis of the acute leukemia samples studied.
EjWRKY6 Is Involved in the ABA-Induced Carotenoid Biosynthesis in Loquat Fruit during Ripening
Authors: Yu Yan , Bao Zeyang , Zhou Qihang , Wu Wei , Chen Wei , Yang Zhenfeng , Wang Li , Li Xuewen , Cao Shifeng , Shi Liyu 
Published: September 2024

The yellow-fleshed loquat is abundant in carotenoids, which determine the fruit’s color, provide vitamin A, and offer anti-inflammatory and anti-cancer health benefits. In this research, the impact of abscisic acid (ABA), a plant hormone, on carotenoid metabolism and flesh pigmentation in ripening loquat fruits was determined. Results revealed that ABA treatment enhanced the overall content of carotenoids in loquat fruit, including major components like β-cryptoxanthin, lutein, and β-carotene, linked to the upregulation of most genes in the carotenoid biosynthesis pathway. Furthermore, a transcription factor, EjWRKY6, whose expression was induced by ABA, was identified and was thought to play a role in ABA-induced carotenoid acceleration. Transient overexpression of EjWRKY6 in Nicotiana benthamiana and stable genetic transformation in Nicotiana tabacum with EjWRKY6 indicated that both carotenoid production and genes related to carotenoid biosynthesis could be upregulated in transgenic plants. A dual-luciferase assay proposed a probable transcriptional control between EjWRKY6 and promoters of genes associated with carotenoid production. To sum up, pre-harvest ABA application could lead to carotenoid biosynthesis in loquat fruit through the EjWRKY6-induced carotenoid biosynthesis pathway.
Advancing Breast Cancer Therapeutics: Targeted Gene Delivery Systems Unveiling the Potential of Estrogen Receptor-Targeting Ligands
Authors: Lee Jung Ro , Kim Young-Min , Kim Eun-Ji , Jang Mi-Kyeong , Park Seong-Cheol 
Published: September 2024

Although curcumin has been well known as a phytochemical drug that inhibits tumor promotion by modulating multiple molecular targets, its potential was not reported as a targeting ligand in the field of drug delivery system. Here, we aimed to assess the tumor-targeting efficiency of curcumin and its derivatives such as phenylalanine, cinnamic acid, coumaric acid, and ferulic acid. Curcumin exhibited a high affinity for estrogen receptors through a pull-down assay using the membrane proteins of MCF-7, a breast cancer cell line, followed by designation of a polymer-based gene therapy system. As a basic backbone for gene binding, dextran grafted with branched polyethylenimine was synthesized, and curcumin and its derivatives were linked to lysine dendrimers. In vitro and in vivo antitumor effects were evaluated using plasmid DNA expressing anti-
bcl-2
short hairpin RNA. All synthesized gene carriers showed excellent DNA binding, protective effects against nuclease, and gene transfection efficiency in MCF-7 and SKBr3 breast cancer cells. Preincubation with curcumin or 17α-estradiol resulted in a marked dose-dependent decrease in gene transfer efficiency and suggested targeting specificity of curcumin. Our study indicates the potential of curcumin and its derivatives as novel targeting ligands for tumor cells and tissues.
Normalización y fiabilidad de la batería para la Evaluación del Procesamiento Lingüístico en el Adulto (PEPLA)
Authors: López-Higuera Amparo , Muñoz-Zambrano Isabel , Guerrero Yeison , Grimaldo-Trujillo Mayra , Sandoval-Calvache Fernanda 
Published: September 2024

La batería para la Evaluación del Procesamiento Lingüístico en el Adulto (PEPLA) promete ser una valiosa herramienta para Colombia, en aras de obtener el perfil lingüístico de adultos con trastornos del lenguaje adquirido, al valorar los procesos básicos de comprensión y producción de mensajes hablados, así como la comprensión y producción de mensajes escritos. Este estudio de fiabilidad tuvo como objetivo contribuir a la normalización de esta batería en una región del suroccidente colombiano. Su diseño metodológico fue cuantitativo, descriptivo, no experimental, y participaron 145 adultos, entre los 18 y 80 años. Las respuestas fueron las esperadas de acuerdo con el contenido de la prueba y se encontraron por encima del percentil 95 de asertividad. El estadístico Alfa de Cronbach α = 0,903 fue indicativo de fiabilidad, clasificada como excelente; por su parte, el estadístico Kruskal-Wallis estableció una relación significativa de las respuestas según el rango de edad y el nivel académico (p < 0.05 y p < 0.00). Se concluye que la batería presenta datos normativos estables y tiene una alta fiabilidad de consistencia interna; por tanto, es posible avanzar en el proceso de estandarización, a fin de satisfacer la necesidad de contar con herramientas validadas en el país.
Educational Intervention "No More Violence" Based on the Reflexive Dialogic Method in the Knowledge about Normativity in Domestic Violence in Peruvian Adolescent Students
Authors: Salas-Sanchez Rosa , Sanga-Solorzano Alberto , Medina-Coronado Daniela , Ninamango-Santos Ninoska , Tamariz Jaime Dora Julia 
Published: September 2024

The normalization of family violence has serious consequences for children, causing emotional disorders, distorting concepts such as respect and obedience, fear, frustrations and complexes. In this sense, this educational intervention was proposed as a mechanism to confront violence and provide Peruvian adolescent students with criteria on legal regulations on family violence to identify and defend themselves in situations of vulnerability and risks of violent abuse. Determine to what extent the educational intervention "No more violence" based on the reflective dialogic method improves knowledge about normativity in domestic violence in Peruvian adolescent students, 2023.The study was applied and quantitative and worked under a pre-experimental design. The population consisted of adolescent students in the fourth grade of high school in a public school in Arequipa. The instrument used was a knowledge test, which underwent the corresponding validation and reliability processes. The results showed that the level of knowledge about regulations on domestic violence improved after the “no more violence” intervention, finding significant differences that favored the experimental group. The reflective dialogic method helps to develop conceptual knowledge and skills for the analysis of situations and the application of regulations.

Received: 30 May 2024 / Accepted: 30 August 2024 / Published: 05 September 2024