bluuebunny/crossref_metadata_2025_split huggingface dataset:
Taming the Production of Bioluminescent Wood Using the White Rot Fungus Desarmillaria Tabescens
Authors: Schwarze Francis W. M. R. , Carvalho Tiago , Reina Giacomo , Greca Luiz Garcia , Buenter Urs , Gholam Zennat , Krupnik Leonard , Neels Antonia , Boesel Luciano , Morris Hugh , Heeb Markus , Huch Anja , Nyström Gustav , Giovannini Giorgia 
Published: September 2024

AbstractAlthough bioluminescence is documented both anecdotally and experimentally, the parameters involved in the production of fungal bioluminescence during wood colonization have not been identified to date. Here, for the first time, this work develops a methodology to produce a hybrid living material by manipulating wood colonization through merging the living fungus Desarmillaria tabescens with nonliving balsa (Ochroma pyramidale) wood to achieve and control the autonomous emission of bioluminescence. The hybrid material with the highest bioluminescence is produced by soaking the wood blocks before co‐cultivating them with the fungus for 3 months. Regardless of the incubation period, the strongest bioluminescence is evident from balsa wood blocks with a moisture content of 700–1200%, highlighting the fundamental role of moisture content for bioluminescence production. Further characterization reveals that D. tabescens preferentially degraded hemicelluloses and lignin in balsa wood. Fourier‐transform infrared spectroscopy reveals a decrease in lignin, while X‐ray diffraction analysis confirms that the cellulose crystalline structure is not altered during the colonization process. This information will enable the design of ad‐hoc synthetic materials that use fungi as tools to maximize bioluminescence production, paving the way for an innovative hybrid material that could find application in the sustainable production of light.
A Micromanipulation‐Actuated Large‐Scale Screening to Identify Optimized Microphysiological Model Parameters in Skeletal Muscle Regeneration
Authors: Chen Xie , Sun Tao , Shimoda Shingo , Wang Huaping , Huang Qiang , Fukuda Toshio , Shi Qing 
Published: September 2024

AbstractHydrogel‐based 3D cell cultures are extensively utilized to create biomimetic cellular microstructures. However, there is still lack of effective method for both evaluation of the complex interaction of cells with hydrogel and the functionality of the resulting micro‐structures. This limitation impedes the further application of these microstructures as microphysiological models (microPMs) for the screening of potential culture condition combinations to enhance the skeletal muscle regeneration. This paper introduces a two‐probe micromanipulation method for the large‐scale assessment of viscoelasticity and contractile force (CF) of skeletal muscle microPMs, which are produced in high‐throughput via microfluidic spinning and 96‐well culture. The collected data demonstrate that viscoelasticity parameters (E* and tanδ) and CF both measured in a solution environment are indicative of the formation of cellular structures without hydrogel residue and the subsequent generation of myotubes, respectively. This study have developed screening criterias that integrate E*, tanδ, and CF to examine the effects of multifactorial interactions on muscle fiber repair under hypoxic conditions and within bioprinted bipennate muscle structures. This approach has improved the quality of hypoxic threshold evaluation and aligned cell growth in 3D. The proposed method is useful in exploring the role of different factors in muscle tissue regeneration with limited resources.
Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties
Authors: McCuskey Samantha R. , Quek Glenn , Vázquez Ricardo Javier , Kundukad Binu , Bin Ismail Muhammad Hafiz , Astorga Solange E. , Jiang Yan , Bazan Guillermo C. 
Published: September 2024

AbstractgLiving materials can achieve unprecedented function by combining synthetic materials with the wide range of cellular functions. Of interest are situations where the critical properties of individual abiotic and biotic elements improve via their combination. For example, integrating electroactive bacteria into conjugated polyelectrolyte (CPE) hydrogels increases biocurrent production. One observes more efficient electrical charge transport within the CPE matrix in the presence of Shewanella oneidensis MR‐1 and more current per cell is extracted, compared to traditional biofilms. Here, the origin of these synergistic effects are examined. Transcriptomics reveals that genes in S. oneidensis MR‐1 related to bacteriophages and energy metabolism are upregulated in the composite material. Fluorescent staining and rheological measurements before and after enzymatic treatment identified the importance of extracellular biomaterials in increasing matrix cohesion. The synergy between CPE and S. oneidensis MR‐1 thus arises from initially unanticipated changes in matrix composition and bacteria adaption within the synthetic environment.
Electrochemically Driven Nickel‐Catalyzed Enantioselective Hydro‐Arylation/Alkenylation of Enones
Authors: Ye Zenghui , Ma Weiyuan , Zhang Xi , Liu Huaqing , Zhang Fengzhi 
Published: September 2024

AbstractHerein, the study reports the first electrochemical nickel‐catalyzed enantioselective hydro‐arylation/alkenylation of enones in an undivided cell with low‐cost electrodes in the absence of external reductants and supporting electrolytes. Aryl bromides/iodides/triflates or alkenyl bromides are employed as electrophiles for the efficient preparation of more than 56 valuable β‐arylated/alkenylated ketones in a simple manner (up to 97% yield, 97% ee). With the advantages of electrochemistry, excellent functional group tolerance and late‐stage modification of complex natural products and pharmaceuticals made the established protocol greener and more economic. Mechanism investigation suggests that a NiI/NiIII cycle may be involved in this electro‐reductive reaction rather than metal reductant driven Ni0/NiII cycle. Overall, the efficient electrochemical activation and turnover of the nickel catalyst avoid the drawbacks posed by the employment of stoichiometric amount of sensitive metal powder reductants.
RuO2 with Short‐Range Ordered Tantalum Single Atoms for Enhanced Acidic Oxygen Evolution Reaction
Authors: Wang Xuefeng , Li Zijian , Jang Haeseong , Chen Changsheng , Liu Shangguo , Wang Liu , Kim Min Gyu , Cho Jaephil , Qin Qing , Liu Xien 
Published: September 2024

AbstractRuthenium Dioxide (RuO2), as one of the most promising alternatives to IrO2, suffers from the severe dissolution and overoxidation of Ru active sites during the acidic oxygen evolution reaction (OER), which hinders its practical application. Herein, the study constructs a short‐range ordered tantalum single atoms‐doped RuO2 catalyst (Ta‐RuO2) with asymmetric Ru‐O‐Ta(‐O‐Ta) active units for the enhanced acidic OER. The Ta‐RuO2 catalyst exhibits superior catalytic activity with an overpotential of 201 mV at 10 mA cm−2 and a long‐lasting stability of 280 h. Physical characterizations combined with electrochemical tests reveal that the incorporation of atomically arranged Ta atoms induces significant tensile strain, effectively optimizing the adsorption strength of oxygen‐containing intermediates by regulating the Ru d‐band center and weakening the Ru‐O covalency, thus boosting the catalytic activity. Furthermore, the formed Ru‐O‐Ta(‐O‐Ta) active local structure is well maintained during the OER process owing to the synergy of strong corrosion resistance of Ta‐O bonds and the electron transfers from Ta to Ru via oxygen bridge stabilizing the Ru sites, contributing to the enhanced stability. This study provides a novel method via incorporation of corrosion‐resistant and short‐range ordered single atoms to significantly enhance the acidic OER stability and activity of cost‐effective catalysts.
Optimizing the prediction of adsorption in metal–organic frameworks leveraging Q‐learning
Authors: Osaro Etinosa , Colón Yamil J. 
Published: September 2024

AbstractThe application of machine learning (ML) techniques in materials science has revolutionized the pace and scope of materials research and design. In the case of metal–organic frameworks (MOFs), a promising class of materials due to their tunable properties and versatile applications in gas adsorption and separation, ML has helped survey the vast material space. This study explores the integration of reinforcement learning (RL), specifically Q‐learning, within an active learning (AL) context, combined with Gaussian processes (GPs) for predictive modeling of adsorption in MOFs. We demonstrate the effectiveness of the RL‐driven framework in guiding the selection of training data points and optimizing predictive model performance for methane and carbon dioxide adsorption, using two different reward metrics. Our results highlight the integration of RL as an AL method for adsorption predictions in MFs, and how it compares to a previously implemented AL scheme.
Exploration of Geographical Environmental Factors Influencing Regional Population Mortality Patterns in China
Authors: Li Tiantian , Li Handong 
Published: September 2024

ABSTRACTObjectivesThe regional population mortality patterns in China exhibit substantial geographical distribution characteristics. This paper aims to explore the impact and mechanisms of geographical environmental factors on regional population mortality patterns.MethodsThis study first utilized the data from China's Seventh Population Census to obtain mortality patterns for the 31 provincial‐level administrative regions. Subsequently, a functional regression method was employed to explore the geographical environmental driving factors of regional mortality patterns.ResultsThe study provides a detailed explanation of the mechanisms and marginal contributions of key geographical environmental factors at different age groups.Conclusions(1) The impact of geographical environmental factors on mortality patterns shows distinct phased characteristics. Mortality patterns before the age of 40 years are hardly influenced by geographical environmental factors, with a noticeable impact beginning at ages 40–69 years and reaching the maximum influence after the age of 70 years. (2) In mortality patterns at ages 40–69 years, average altitude have the most substantial impact, followed by extreme low‐temperature days and PM2.5 concentration. In mortality patterns at ages 70–94 years, high‐temperature days have the greatest influence, followed by the impact of SO2 concentration. (3) In comparisons based on gender, socioeconomic factors, and geographical environmental factors, gender and urban–rural differences have the most significant impact on regional population mortality patterns, followed by the influence of other socioeconomic factors, with geographical environmental factors having a relatively smaller impact.
Detection of sex‐specific glutamate changes in subregions of hippocampus in an early‐stage Alzheimer's disease mouse model using GluCEST MRI
Authors: Soni Narayan Datt , Swain Anshuman , Juul Halvor , Cao Quy , Haris Mohammad , Wolk David A. , Lee Virginia M.‐Y. , Detre John A. , Nanga Ravi Prakash Reddy , Reddy Ravinder 
Published: September 2024

AbstractINTRODUCTIONRegional glucose hypometabolism resulting in glutamate loss has been shown as one of the characteristics of Alzheimer's disease (AD). Because the impact of AD varies between the sexes, we utilized glutamate‐weighted chemical exchange saturation transfer (GluCEST) magnetic resonance imaging (MRI) for high‐resolution spatial mapping of cerebral glutamate and investigated subregional changes in a sex‐specific manner.METHODSEight‐month‐old male and female AD mice harboring mutant amyloid precursor protein (APPNL‐F/NL‐F:n= 36) and wild‐type (WT:n= 39) mice underwent GluCEST MRI, followed by proton magnetic resonance spectroscopy (1H‐MRS) in hippocampus and thalamus/hypothalamus using 9.4T preclinical MR scanner.RESULTSGluCEST measurements revealed significant (p ≤ 0.02) glutamate loss in the entorhinal cortex (% change ± standard error: 8.73 ± 2.12%), hippocampus (11.29 ± 2.41%), and hippocampal fimbriae (19.15 ± 2.95%) of male AD mice. A similar loss of hippocampal glutamate in male AD mice (11.22 ± 2.33%;p= 0.01) was also observed in1H‐MRS.DISCUSSIONSGluCEST MRI detected glutamate reductions in the fimbria and entorhinal cortex of male AD mice, which was not reported previously. Resilience in female AD mice against these changes indicates an intact status of cerebral energy metabolism.HighlightsGlutamate levels were monitored in different brain regions of early‐stage Alzheimer's disease (AD) and wild‐type male and female mice using glutamate‐weighted chemical exchange saturation transfer (GluCEST) magnetic resonance imaging (MRI).Male AD mice exhibited significant glutamate loss in the hippocampus, entorhinal cortex, and the fimbriae of the hippocampus.Interestingly, female AD mice did not have any glutamate loss in any brain region and should be investigated further to find the probable cause.These findings demonstrate previously unreported sex‐specific glutamate changes in hippocampal sub‐regions using high‐resolution GluCEST MRI.
Verbesserte Propylen/Propan‐Trennung durch eine Anilin‐modifizierte ZIF‐8‐Membran: Gittersteifigkeit und Adsorptionszentren
Authors: Zhang Xin , Hai Guangtong , Yao Yujian , Zhang Zheng , Cao Qingbin , Tan Hongxin , Ding Li , Han Jiuli , Wei Yanying , Caro Jürgen , Wang Haihui 
Published: September 2024

AbstractMembranen auf Basis metallorganischer Gerüststrukturen (metal–organic frameworks=MOFs) ermöglichen molekulare Trennungen und stoßen daher auf erhebliches Forschungsinteresse. Die kristallographisch begründete Nanoporenstruktur und selektive Adsorptionswechselwirkungen bestimmen die Gastrennleistung von MOF‐Membranen. In unserem Syntheseansatz wurde der ZIF‐8‐Syntheselösung Anilin in unterschiedlichen Konzentrationen zugesetzt. Anilin, das in den großen ZIF‐8‐Hohlräumen verkapselt ist, interagiert stark mit dem 2‐Methylimidazolat‐Linker, was sowohl zu einer Verschiebung der kristallographisch flexiblen ZIF‐8‐Phase I_43 m zur steifen Phase Cm führt (Rietveld‐Verfeinerung der Röntgenbeugungsmuster) als auch eine selektive Propen‐Adsorption ermöglicht. Es wurde eine Anilin‐modifizierte ZIF‐8 (Anix‐ZIF‐8)‐Membran durch schnelle Elektro‐Synthese hergestellt, die eine gute Propylen/Propan‐Trennungsselektivität von bis zu 85 aufweist. Die Berechnung der Wechselwirkungsenergie zwischen Anilin und den verschiedenen kristallographischen Phasen von ZIF‐8 mit Hilfe der Dichtefunktionaltheorie (DFT) bestätigt weiterhin, dass Anilin nicht nur die Bildung der kristallographischen Cm‐Phase fördert, sondern auch die Adsorptionsselektivität von Propylen gegenüber Propan erhöht. Durch die Anilinmodifikation wird die kristallographische Phasenstruktur von ZIF‐8 effektiv beeinflusst und ein molekulares Propylen/Propan‐Sieben ermöglicht.
Enhanced Propylene/Propane Separation via Aniline‐Decorated ZIF‐8 Membrane: Lattice Rigidity Adjustment and Adsorption Site Introduction
Authors: Zhang Xin , Hai Guangtong , Yao Yujian , Zhang Zheng , Cao Qingbin , Tan Hongxin , Ding Li , Han Jiuli , Wei Yanying , Caro Jürgen , Wang Haihui 
Published: September 2024

AbstractMetal–organic framework (MOF)‐based membranes excel in molecular separation, attracting significant research interest. The crystallographic microstructure and selective adsorption capacity of MOFs closely correlate with their gas separation performance. Here, aniline was added to the ZIF‐8 synthesis in varying concentrations. Aniline, encapsulated within ZIF‐8 cavities, interacts strongly with the 2‐methylimidazole linker, resulting in both a shift in crystallographic phase from I_43 m to Cm in Rietveld refinement of X‐ray diffraction (XRD) patterns and the selective adsorption behavior between propylene and propane. Consequently, an aniline decorative ZIF‐8 (Anix‐ZIF‐8) membrane was prepared using a fast current‐driven synthesis method, which exhibits good propylene/propane separation selectivity of up to 85. Calculation of the interaction energy between aniline and the various crystallographic phases of ZIF‐8 using density functional theory (DFT) further verifies that aniline not only promotes the formation of crystallographic Cm phase, but also enhances the adsorption selectivity of propylene over propane. Aniline modification effectively tunes the crystallographic microstructure of ZIF‐8, thereby, improving molecular sieving capabilities.
Asymmetric Synthesis of Chiral Calix[4]arenes with Both Inherent and Axial Chirality via Cobalt‐Catalyzed Enantioselective Intermolecular C−H Annulation
Authors: Qian Pu‐Fan , Zhou Gang , Hu Jia‐Heng , Wang Bing‐Jie , Jiang Ao‐Lian , Zhou Tao , Yuan Wen‐Kui , Yao Qi‐Jun , Chen Jia‐Hao , Kong Ke‐Xin , Shi Bing‐Feng 
Published: September 2024

AbstractInherently chiral calixarenes have garnered significant attention due to their distinctive properties, yet the development of efficient catalytic asymmetric synthesis methods remains a critical challenge. Herein, we report the asymmetric synthesis of calix[4]arenes featuring inherent or both inherent and axial chirality via a cobalt‐catalyzed C−H activation/annulation strategy in high yield with excellent enantio‐ and diastereoselectivity (up to >99 % ee and >20 : 1 dr). Electrooxidation was also suitable for this transformation to obviate the sacrificial metal oxidants, underscoring the environmentally friendly potential of this approach. A key octahedral cobaltacycle intermediate was synthesized and characterized, providing valuable insights into the mode of enantio‐ and diastereocontrol of this protocol. Noteworthy photoluminescence quantum yields of up to 0.94 were measured, underscoring the potential of these compounds in the domain of organic fluorescent materials.
Enhancement of Intracellular Cholesterol Efflux in Chondrocytes Leading to Alleviation of Osteoarthritis Progression
Authors: Lee Gyuseok , Yang Jiye , Kim Su‐Jin , Tran Thanh‐Tam , Lee Sun Young , Park Ka Hyon , Kwon Seung‐Hee , Chung Ki‐Ho , Koh Jeong‐Tae , Huh Yun Hyun , Seon Jong‐Keun , Kim Hyun Ah , Chun Jang‐Soo , Ryu Je‐Hwang 
Published: September 2024

ObjectiveOsteoarthritis (OA) is the most common degenerative disease worldwide, with no practical means of prevention and limited treatment options. Recently, our group unveiled a novel mechanism contributing to OA pathogenesis in association with abnormal cholesterol metabolism in chondrocytes. In this study, we aimed to establish a clinical link between lipid profiles and OA in humans, assess the effectiveness of cholesterol‐lowering drugs in suppressing OA development in mice, and uncover the cholesterol‐lowering mechanisms that effectively impede OA progression.MethodsFive clinically approved cholesterol‐lowering drugs (fenofibrate, atorvastatin, ezetimibe, niacin, and lomitapide) were injected into the knee joints or administered with diet to mice with OA who underwent destabilization of the medial meniscus induction and were fed a 2% high‐cholesterol diet. Gene expression linked to cholesterol metabolism was determined using microarray analysis. Furthermore, the in vivo functions of these genes were explored through intra‐articular injection of either its inhibitor or adenovirus.ResultsLogistic regression analysis confirmed a close relationship between the diagnostic criteria of hyperlipidemia based on serum lipid levels and OA incidence. Among the cholesterol‐lowering drugs examined, fenofibrate exerted the most significant protective effect against cartilage destruction, which was attributed to elevated levels of high‐density lipoprotein cholesterol that are crucial for cholesterol efflux. Notably, cholesterol efflux was suppressed during OA progression via down‐regulation of apolipoprotein A1–binding protein (AIBP) expression. Overexpression of AIBP effectively inhibits OA progression.ConclusionOur results suggest that restoration of cholesterol homeostasis to a normal state through administration of fenofibrate or AIBP overexpression, both of which induce cholesterol efflux, offers an effective therapeutic option for patients with OA.
The ARTISTIC Battery Manufacturing Digitalization Initiative: From Fundamental Research to Industrialization
Authors: Troncoso Javier F. , Zanotto Franco M. , Galvez‐Aranda Diego E. , Zapata Dominguez Diana , Denisart Lucie , Franco Alejandro A. 
Published: September 2024

AbstractOur ARTISTIC project was born in 2018 to improve the efficiency of lithium‐ion battery cell manufacturing process through computational modelling, allowing the research and development of new digital tools to accelerate the optimization of this process. Thanks to the development and use of innovative numerical models, machine learning algorithms and virtual and mixed reality tools, we could significantly advance the understanding of manufacturing/battery cell performance relationships. However, scientific research by itself is not enough to bring innovations into practical applications for society. The creation of spin‐offs or start‐ups can ease the transition from research to application, since it allows scaling up the research outputs into products or services ready‐to‐use by the customers. In this Concept, we discuss the benefits of this transition, we introduce the research findings obtained in the last years within the framework of our ARTISTIC project, and our actions to move from our research to industrial products.
Prognostic value of tumor‐infiltrating lymphocytes and PD‐L1 expression in esophageal squamous cell carcinoma
Authors: Hu Jie , Toyozumi Takeshi , Murakami Kentaro , Endo Satoshi , Matsumoto Yasunori , Otsuka Ryota , Shiraishi Tadashi , Iida Shinichiro , Morishita Hiroki , Makiyama Tenshi , Nishioka Yuri , Uesato Masaya , Hayano Koichi , Nakano Akira , Matsubara Hisahiro 
Published: September 2024

AbstractBackgroundTumor cells (TC) participate in tumor progression by altering the immune responses in the tumor microenvironment. However, the clinical relevance and prognostic effect of PD‐L1 expression and tumor‐infiltrating lymphocytes (TILs) in esophageal squamous cell carcinoma (ESCC) are unknown. The purpose of this study was to investigate the interactions and clinical significance of PD‐L1 expression and TILs in ESCC.MethodsTissue specimens were collected from 126 patients with ESCC who underwent curative esophagectomy. Immunohistochemical analysis and multiplex immunofluorescence for CD4, CD8, CD25, FOXP3, and PD‐L1 in the tumor were used to identify multiple tumor‐infiltrating immune cells (TIIC), Tregs, and TC.ResultsPD‐L1 was expressed in tumor cells (PD‐L1 TC). PD‐L1 TIIC and PD‐L1 TC affected the biological behavior of TC. The positive expression rate of PD‐L1 TC and CD8+ TILs was 27.8% (35/126) and 31.7% (40/126), respectively. Kaplan–Meier analysis showed that overall survival (OS) was significantly associated with decreased CD8+ TILs and PD‐L1 TC‐positive expression, which promote ESCC progression and metastasis.ConclusionTumor depth, CD8, and PD‐L1 TC were independent prognostic factors in ESCC, and a predictive nomogram with these three risk factors improved the accuracy of predicting OS in patients with ESCC after surgical resection. The conjoint analysis of multiple immune‐related factors is beneficial for stratifying patient survival risk.