bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application Of Microwaves and Megasound to Olive Paste in an Industrial Olive Oil Extraction Plant: Impact on Virgin Olive Oil Quality and Composition
Authors: Leone Alessandro , Romaniello Roberto , Tamborrino Antonia , Urbani Stefania , Servili Maurizio , Amarillo Miguel , Grompone Maria A. , Gambaro Adriana , Juliano Pablo 
Published: August 2017

Traditional olive oil processing includes batch olive paste kneading for 30–60 min. Microwave (MW) treatment of the olive paste have recently shown to continuously provide faster conditioning, while megasound (MS) treatment have been shown to enhance oil extractability. In the present work the virgin olive oil standard parameters, sensory descriptors, and phenolic and headspace composition upon treatment of oils obtained using novel process enhancement interventions, and their combination in an industrial plant is examined. Standard parameters are within the international specifications. The Control and MW process provide similar phenolic composition but a post MS intervention significantly increased total phenolic content, and decrease total C5 and C6 aldehydes in the MW treated paste. These results encourage further developments for a combined continuous MW and megasonic conditioning technology to fasten the olive oil extraction, to enhance yields, and total phenolic content.Practical Applications: Microwave and megasounds are two technologies easily implementable on industrial olive oil extraction plant. They permit to simplify the entire plant and could replace totally the malaxers section. This new technology allows having a uniform paste treatment in continuous mode, enhancing the olive oil quality.Microwaves and megasonics machine are employed to conditioning the olive paste. Chemical analyses on obtained oils, compared to the traditional technology, show that the new technologies allow producing good quality oils. These results encourage further developments for a combined continuous microwave and megasonic conditioning technology to fasten the olive oil extraction, to enhance yields, and total phenolic content.
What do I gain from joining crowds? Does self‐expansion help to explain the relationship between identity fusion, group efficacy and collective action?
Authors: Besta Tomasz , Jaśkiewicz Michał , Kosakowska‐Berezecka Natasza , Lawendowski Rafał , Zawadzka Anna Maria 
Published: August 2017

AbstractFour studies were carried out to examine how identity fusion, self‐ and group efficacy, and collective action are related and what role self‐expansion plays in these relationships. In the pilot study, participants recalled their experience of participating in mass gatherings. The three other studies were conducted during mass gatherings organized for collective purposes: a music concert (Study 1), a bicycle activist event (Study 2), and Equality Days (Study 3). The results showed (a) a significant positive relationship between personal and group identity fusion, self‐expansion, and self‐efficacy (Study 1); (b) a significant mediating effect of self‐expansion on the relationship between personal and group identity fusion and group efficacy (Studies 1 and 2); and (c) a significant mediating effect of self‐ expansion and group efficacy on the relationship between identity fusion and collective action tendency (Studies 2 and 3).
Tellurite Carbon Paste Sensors: Microscopic Analysis Provides New Insights on the Nature of the Interaction Between the Ionophore and Analytical Species
Authors: Issa Yousry M. , M. Abdel‐Fattah Hussein , R. Shehab Ola , B. Mohamed Nahla 
Published: August 2017

AbstractIn this work, scanning electron microscopy (SEM) was used for the characterization of chemically modified carbon paste sensors (CMCPSs), with the aim of understanding their chemical and electrochemical properties. The work also provides a microscopic study on the role of the binder, type of carbon, amount and nature of modifier, composition of the carbon paste and its impact on the electrochemical performance of the sensor. In addition, energy dispersive X‐ray spectroscopy (EDX) was utilized for the direct observation of the interaction between the chemical modifier and the carbon paste. This has enabled the fabrication of new sensitive and selective chemically modified carbon paste sensors that were successfully applied for the detection of tellurite ions in pure solutions, environmental and biological samples. This work provides new insights on the rational design and integration of carbon paste sensors for a variety of applications.
Recent developments in open tubular capillary electrochromatography from 2016 to 2017
Authors: Tarongoy Faustino M. , Haddad Paul R. , Quirino Joselito P. 
Published: August 2017

AbstractInterest in open‐tubular capillary electrochromatography (OT‐CEC) continues to thrive because of the inherent advantage of OT‐CEC combining the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography. For the period 2016 to 2017, novel materials have been developed as first‐time stationary phases for OT‐CEC and are grouped in this review as polymer‐based materials, frameworks, nanoparticles, graphene‐based materials, and biomaterials. Coating and fabrication methods mostly rely on covalent coating strategies while non‐covalent immobilisation strategies like electrostatic assembly are notably still being employed. The concern of overcoming phase ratio challenges in OT‐CEC coatings have also generated adoption of combined coating strategies including multi‐layering, layer‐by‐layer self‐assembly and methods adapted from nanofilm fabrications like epitaxial growth, liquid phase deposition, or nucleation of crystal growth. The emergence of non‐conventional coating characterisation methods such as transmission electron microscopy, X‐ray diffraction or X‐ray photoelectron spectroscopy is also discussed.
Evaluation of wastewater treatment by ozonation for reducing the toxicity of contaminants of emerging concern to rainbow trout (Oncorhynchus mykiss)
Authors: Maya Nicholas , Evans Jaden , Nasuhoglu Deniz , Isazadeh Siavash , Yargeau Viviane , Metcalfe Chris D. 
Published: August 2017

Abstract

Although conventional wastewater treatment technologies are effective at removing many contaminants of emerging concern (CECs) from municipal wastewater, some contaminants are not removed efficiently. Ozonation may be a treatment option for reducing the concentrations of recalcitrant CECs in wastewater, but this process may generate toxic transformation products. In the present study, we conducted semibatch experiments to ozonate municipal wastewater effluent spiked with 5 commonly detected CECs. The purpose of the present study was to evaluate whether ozonation increased or decreased biological responses indicative of sublethal toxicity in juvenile rainbow trout (Oncorhynchus mykiss) injected intraperitoneally (i.p.) with extracts prepared from ozonated and nonozonated wastewater effluent. Blood, liver, and brain tissues were collected from the fish at 72 h post injection for analysis of a battery of biomarkers. In fish i.p. injected with the extracts from nonozonated wastewater effluent, significant induction of plasma vitellogenin (VTG) was observed, but ozonation of the municipal wastewater effluent spiked with CECs significantly reduced this estrogenic response. However, in fish injected with extracts from spiked municipal wastewater effluent after ozonation, the balance of hepatic glutathione in its oxidized (glutathione disulfide [GSSG]) form was altered, indicating oxidative stress. Levels of the neurotransmitter serotonin were significantly elevated in brain tissue from trout injected with the extracts from ozonated spiked municipal wastewater effluent, a biological response that has not been previously reported in fish. Other in vivo biomarkers showed no significant changes across treatments. These results indicate that ozonation reduces the estrogenicity of wastewater, but may increase other sublethal responses. The increase in biomarker responses after ozonation may be because of the formation of biologically active products of transformation of CECs, but further work is needed to confirm this conclusion. Environ Toxicol Chem 2018;37:274–284. © 2017 SETAC
Sex‐biased dispersal of human ancestors
Authors: Sugiyama Yukimaru 
Published: August 2017

AbstractSome anthropologists and primatologists have argued that, judging by extant chimpanzees and humans, which are female‐biased dispersers, the common ancestors of humans and chimpanzees were also female‐biased dispersers. It has been thought that sex‐biased dispersal patterns have been genetically transmitted for millions of years. However, this character has changed many times with changes in environment and life‐form during human evolution and historical times. I examined life‐form and social organization of nonhuman primates, among them gatherers (foragers), hunter‐gatherers, agriculturalists, industrialists, and modern and extant humans. I conclude that dispersal patterns changed in response to environmental conditions during primate and human evolution.
A randomized, placebo‐controlled trial of cenicriviroc for treatment of nonalcoholic steatohepatitis with fibrosis
Authors: Friedman Scott L. , Ratziu Vlad , Harrison Stephen A. , Abdelmalek Manal F. , Aithal Guruprasad P. , Caballeria Juan , Francque Sven , Farrell Geoffrey , Kowdley Kris V. , Craxi Antonio , Simon Krzysztof , Fischer Laurent , Melchor‐Khan Liza , Vest Jeffrey , Wiens Brian L. , Vig Pamela , Seyedkazemi Star , Goodman Zachary , Wong Vincent Wai‐Sun , Loomba Rohit , Tacke Frank , Sanyal Arun , Lefebvre Eric 
Published: August 2017

The aim of this study was to evaluate cenicriviroc (CVC), a dual antagonist of CC chemokine receptor types 2 and 5, for treatment of nonalcoholic steatohepatitis (NASH) with liver fibrosis (LF). A randomized, double‐blind, multinational phase 2b study enrolled subjects with NASH, a nonalcoholic fatty liver disease activity score (NAS) ≥4, and LF (stages 1‐3, NASH Clinical Research Network) at 81 clinical sites. Subjects (N = 289) were randomly assigned CVC 150 mg or placebo. Primary outcome was ≥2‐point improvement in NAS and no worsening of fibrosis at year 1. Key secondary outcomes were: resolution of steatohepatitis (SH) and no worsening of fibrosis; improvement in fibrosis by ≥1 stage and no worsening of SH. Biomarkers of inflammation and adverse events were assessed. Full study recruitment was achieved. The primary endpoint of NAS improvement in the intent‐to‐treat population and resolution of SH was achieved in a similar proportion of subjects on CVC (N = 145) and placebo (N = 144; 16% vs. 19%, P = 0.52 and 8% vs. 6%, P = 0.49, respectively). However, the fibrosis endpoint was met in significantly more subjects on CVC than placebo (20% vs. 10%; P = 0.02). Treatment benefits were greater in those with higher disease activity and fibrosis stage at baseline. Biomarkers of systemic inflammation were reduced with CVC. Safety and tolerability of CVC were comparable to placebo. Conclusion: After 1 year of CVC treatment, twice as many subjects achieved improvement in fibrosis and no worsening of SH compared with placebo. Given the urgent need to develop antifibrotic therapies in NASH, these findings warrant phase 3 evaluation. (Hepatology 2018;67:1754‐1767).
Permissible emission limit estimation via iterative back-calculation: Case of Assaluyeh energy zone, southern Iran
Authors: Khajehpour Hossein , Saboohi Yadollah , Tsatsaronis George 
Published: August 2017

Abstract

In the present research work, an environmental policy procedure for setting a cap on emissions, as a crucial step in any total emission control system, has been provided and evaluated. It is shown that general regulations on emission intensities and rates do not guarantee that ambient air quality standards are met in intense industrial zones. Local emission limits are necessary to meet ambient air quality standards in these zones. To that end, we used dispersion simulators to back-calculate pollutant concentration thresholds for a large and intense energy system in the Assaluyeh region of southern Iran. Verified modeling results indicate 218 d of pollutant concentration threshold exceedance in Assaluyeh in a simulated year. Back-calculation to assess the total permissible emission level indicates the need for 68% reduction in total emission to meet ambient air quality standards. We then used the model to help identify effective control strategies, including emission reductions and appropriate timing of specific operations. Integr Environ Assess Manag 2018;14:130–138. © 2017 SETAC

Key Points
Environmental guidelines on emission intensities and emission rates are not guarantors of local ambient air quality standards in intense industrial zones; hence, local regulations based on environmental impact assessment tools are required. In the presence of a variety of factors affecting concentrations (e.g., numerous sources, complex topography, variability of meteorological conditions and background concentrations, and the structure of standards), the use of analytical models is vital for estimation of permissible emission limit. A procedure for gradual back-calculation of air quality health criteria is examined in a highly polluted energy zone in Iran, which has become a serious concern for national policy makers. The findings show the need for 68% reduction in emissions to achieve national ambient air quality standards. Due to the presence of background dust during the simulated year, even with no industrial activity, the international air quality guidelines (i.e.,World Health Organization) may not be attained.
Use of first‐trimester placenta growth factor concentration to predict hypertensive disorders of pregnancy in a low‐risk Asian population
Authors: Kumar Manisha , Sharma Karuna , Singh Shalini , Singh Ritu , Singh Abha , Bhattacharjee Jayashree 
Published: August 2017

AbstractObjectiveTo determine the value of placenta growth factor (PlGF) for predicting hypertensive disorders of pregnancy (HDP) in a low‐risk population when used either alone or in combination with other markers.MethodsA prospective observational cohort study was conducted among women with singleton pregnancy in the first trimester in New Delhi, India, between October 1, 2013, and September 30, 2016. First‐trimester measures included maternal history, body mass index (BMI), mean arterial pressure (MAP), Doppler uterine artery pulsatility index, and serum levels of biomarkers (including PlGF).ResultsAmong 1725 women, 208 (12.1%) developed HDP; 74 (35.6%) of these cases were early onset. Mean PlGF levels were significantly lower among cases than among controls (30.42 ± 10.22 vs 37.31 ± 13.28 pg/mL; P<0.001). As a biomarker for prediction of HDP, PlGF fared better than pregnancy‐associated plasma protein A (PAPP‐A): area under the curve 0.685 (95% confidence interval [CI] 0.620–0.750; P<0.001) versus 0.659 (95% CI 0.593–0.726; P<0.001). The highest odds ratio was found for MAP (8.369, 95% CI 5.752–12.215). The combination of PlGF, PAPP‐A, BMI, MAP, and Doppler uterine artery pulsatility index provided an area under the receiver operating characteristic curve of 0.876 (95% CI 0.833–0.919; P<0.001).ConclusionCombining PlGF concentration with biochemical and biophysical markers provided a good screening test for HDP during the first trimester.
Short-Term Teriparatide and Recombinant Human Bone Morphogenetic Protein-2 for Regenerative Approach to Medication-Related Osteonecrosis of the Jaw: A Preliminary Study
Authors: Jung Junho , Yoo Hee-Young , Kim Gyu-Tae , Lee Jung-Woo , Lee Yeon-Ah , Kim Deog-Yoon , Kwon Yong-Dae 
Published: August 2017

ABSTRACT

Our objective was to examine whether adjunct teriparatide administration and local application of recombinant human bone morphogenetic protein-2 (rhBMP-2) is beneficial for the regeneration of jaw bone in patients with medication-related osteonecrosis of the jaw (MRONJ). This study enrolled 17 patients diagnosed with MRONJ. All patients received sequestrectomy under general or local anesthesia with suspension of bisphosphonate. The bone regeneration ratio was compared on cone beam computed tomography (CBCT) scans, acquired immediately post-operation and after 6 months. The patients were divided into groups, based on their treatment regimens: teriparatide combined with rhBMP-2 (parathyroid hormone [PTH]+BMP), rhBMP-2 (BMP), and the control. Biochemical markers were also evaluated at the baseline (T0), 1 month (T1), and 3 months (T2) after surgery. Significant increase was observed in the values of the biochemical markers, serum osteocalcin, and serum C-terminal telopeptide cross-link of type I collagen, within 3 months of surgery in the PTH+BMP group, whereas the mean value in the BMP group did not show a significant change. In all groups, the MRONJ lesions were healed and new bone formation was detected in the CBCT images. The regeneration ratio was significantly greater in the group PTH+BMP than in the BMP and control groups. Significantly greater amount of bone formation was observed in the group PTH+BMP than in the BMP and control groups. Local application of rhBMP-2 alone also had a beneficial effect on bone regeneration but was not more significant than control. Based on these findings, administration of short-term teriparatide with rhBMP-2 in MRONJ patients may maximize the regeneration of bone after surgery. © 2017 American Society for Bone and Mineral Research.
MAPK8 regulates chicken male germ cell differentiation through JNK signaling pathway
Authors: Wang Yingjie , Bi Yulin , Zuo Qisheng , Zhang Wenhui , Li Dong , He Na‐na , Cheng Shaoze , Zhang Ya‐ni , Li Bichun 
Published: August 2017

AbstractThe study aims to analyze the key signaling pathways in regulating the process of embryonic stem cells (ESCs) differentiation into spermatogonial stem cells (SSCs). We explored the specific regulating mechanisms of C‐Jun amino‐terminal kinase (JNK) signaling in this process. Interference/overexpression of MAPK8 allows the JNK signaling pathway to be blocked/activated. In Retinoic acid (RA) induced in vitro differentiation assays, the expression of germ cell marker genes, cvh, c‐kit, integrin α6 and integrin β1, was observed to upregulate while activating JNK signaling significantly. Fluorescence Activated Cell Sorting (FACs) analysis showed that the proportion of cvh+ and integrin α6+cells in the overexpression group was significantly higher than which in the RA + shRNA‐MAPK8 group. In in vivo situations, shRNA‐MAPK8 could stably express in chicken embryos and significantly down‐regulate expression of MAPK8 and downstream genes in JNK signaling pathway. With PAS stain, we found that PGCs (primordial germ cells) was significantly decreased after inhibiting MAPK8. With real‐time quantitative PCR (qRT‐PCR) and Western Blot, we identified that reproductive related genes expression was significantly suppressed after inhibiting MAPK8 in vivo. We preliminarily concluded that knockdown/ overexpression of MAPK8 could affect differentiation of ESC by inhibiting/activating JNK signal.
Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer
Authors: Wang Yu , Zhang Hong , Li Xu , Chen Wei 
Published: August 2017

AbstractUrothelial carcinoma associated 1α (UCA1α) is a novel long non‐coding RNA (lncRNA) that regulates bladder cancer proliferation, migration, and invasion. The target genes of UCA1α have, however, not been identified. To address this, a pCDNA3.1(+)‐UCA1α over‐expression vector was transfected into UM‐UC‐2 bladder cancer cells. Genes differentially expressed between pCDNA3.1(+)‐UCA1α and pCDNA3.1(+) transfected cell were then detected by microarray and bioinformatics analysis. A total of 71 differentially expressed genes were identified, including 52 up‐regulated genes and 19 down‐regulated genes. As expected, the lncRNA UCA1α expression level was significantly increased when compared to that of pCDNA3.1(+) transfected cells. The five most significantly up‐regulated and five most significantly down‐regulated genes were selected, and their expression levels were also assessed by real time quantitative polymerase chain reaction and Western blot. The mRNA and protein expression levels of FOXI3 and GSTA3 were found to be significantly increased, and those of MED18 and TEX101 were found to be significantly decreased. Gene ontology (GO) clustering identified several significant biological processes, cellular components, and molecular functions, associated with lncRNA UCA1α over‐expression. The differentially expressed genes were involved in several significant pathways as shown by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway clustering. Cell proliferation activity was significantly increased following overexpression of lncRNA UCA1α increasing over culture time. The present study identifies, for the first time, potential target genes for lncRNA UCA1α in bladder cancer, and provides a significant reference for studying the role of lncRNA UCA1α in bladder cancer.
Micro‐vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia‐reperfusion injury in rats after cardiac death renal transplantation
Authors: Wu Xiaoqiang , Yan Tianzhong , Wang Zhiwei , Wu Xuan , Cao Guanghui , Zhang Chan , Tian Xiangyong , Wang Junpeng 
Published: August 2017

AbstractThe purpose of the present study was to investigate the possible therapeutic effects of the human Wharton‐Jelly mesenchymal stromal cells derived micro‐vesicles (hWJMSCs‐MVs) on renal ischemia‐reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post‐transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)‐α, and interleukin (IL)‐10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri‐chrome straining and alpha‐smooth muscle actin (α‐SMA) staining. The infiltration of inflammatory cells was detected by CD68+ staining. The transforming growth factor (TGF)‐β, hepatocyte growth factor (HGF), and α‐SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs‐MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68+ macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α‐SMA and TGF‐β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.
Cytokine profiling of MCF‐7 cell line 2D, progressive 3D, and 3D revert cultures
Authors: Subramaniyan Aishwarya , Maddaly Ravi 
Published: August 2017

AbstractCancer cytokines are known to mediate several complex cancer cell physiologies. Also, cancer cells themselves are known to secrete cytokines whose expressions and net inducible results are controlled by a variety of factors. We profiled a few cytokines secreted by 2D, 3D aggregates, and the 3DRs of MCF‐7 cell line at various time points. Several cytokines were seen more expressed by 3D cultures on the 4th day and IL‐10 peaked on the day 1 of 3D cultures while TNF‐α level peaked on the 7th day. α‐Defensin, SDF‐7, and TGF‐β also showed markedly higher levels. There was a reduced expression of IL‐6 and IL‐17 by the 3DRs. TGF‐β did not show much change among the 2D, progressive 3D, and 3DR cultures. Utilizing 3DRs as study material will be a significant extension of the ways cells lines can be used for research.