bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: CPEB3 can regulate seizure susceptibility by inhibiting the transcriptional activity of STAT3 on NMDARs expression
Authors: You Zhipeng , Huang Cong , Wei Fan , Li Jiran , Liu Yang , Liu Xingan , Fan Zhijie , Gao Xiaoying , Sun Jiahang 
Published: September 2024

Abstract

Background
The pathogenesis of epilepsy is complex, and current antiepileptic drugs do not effectively control the seizures. Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) regulates neuronal excitability, but its mechanism of action in epilepsy is not clear. In this paper, we investigated the effect of CPEB3 on seizures and elucidated its underlying molecular mechanism.
Methods
Bioinformatics-based search for genes closely associated with epilepsy. Changes in expression and cellular localization of CPEB3 in epilepsy were verified by Western blotting (WB) and Immunofluorescence staining. Subsequently, The adeno-associated virus was employed to overexpress or Knockdown in mice. Behavioral experiments verified the effect of CPEB3 on epileptic phenotype, and the molecular mechanism of CPEB3 affecting epileptic phenotype was explored by WB, Real-time quantitative polymerase chain reaction (RT-qPCR), RNA immunoprecipitation (RIP), and Chromatin immunoprecipitation (CHIP).
Results
The results were that CPEB3 was downregulated epilepsy in model mice and patients with temporal lobe epilepsy and co-expressed with neurons. Behavioral experiments have shown that CPEB3 negatively regulates seizure susceptibility and excitability. In addition, CPEB3 can also bind to the mRNA of signal transducer and activator of transcription 3 (STAT3) and inhibit its translation, resulting in lower levels of STAT3 and p-STAT3, reduced nuclear translocation of STAT3, and decreased STAT3-mediated transcriptional activity of GluN1, GluN2A, and GluN2B, suppressing the expression of NMDAR subunits and attenuating epilepsy phenotype.
Conclusion
These findings confirm that CPEB3 can alter the excitability and susceptibility of epilepsy by inhibiting the translation of STAT3 and inhibiting its transcription to NMDAR. These results provide new ideas and therapeutic targets to treat epilepsy.
Successful perinatal management in pregnant women with R9 limb-girdle muscular dystrophy with a left ventricular ejection fraction of 27%: A case report
Authors: Xie Wenchi , Mei Jie 
Published: September 2024

Abstract

A 29-year-old patient with muscle weakness at 26 + 5 weeks of pregnancy was hospitalized for heart failure, and genetic testing revealed that she had R9 limb-girdle muscular dystrophy (LGMDR9). Because LGMDR9 is associated with cardiac and respiratory dysfunction, to prevent further deterioration of cardiac function and muscle weakness, we promoted fetal lung maturation and performed an emergency cesarean section at 29 + 2 weeks. This case highlights the importance of choosing the right mode of delivery, timing of delivery, and early genetic testing for LGMDR9 patients.
In Search of a Characterisation of Frailty: Applying Exploratory Factor Analysis to Eldery Preoperative Patients
Authors: Miguel-Ruano Guillermo , Aymerich-De-Franceschi María , García-Aroca Miguel Ángel , Benitez Edgar , Pérez-Fernández Nicolás , Álvarez-Avello José Manuel 
Published: September 2024

Abstract

Background
Frailty is a useful prognostic concept which has spread to many clinical settings, including perioperative medicine. However, there is no consensus on its definition. This situation could impair its screening and the correction of underlying disturbances that have an influence on the clinical course of elderly patients.
Methods
In order to achieve a more precise characterisation of frailty, an exploratory factor analysis (EFA) was performed on the variables of eight frailty scales: Clinical Frailty Scale (CFS), Frail scale, Edmonton scale, Fried criteria, Robinson scale, Risk Analysis Index, the Frailty Index and the modified-Frailty Index. Later, a concordance study between the factors found in EFA and frailty according to the CFS (≥ 4 points) was conducted. 109 preoperative patients aged 65 years or older (60% men) were included, and data were collected from medical history, physical and laboratory tests. Our aim was to explain the covariance of frailty-related variables by identifying the factors that influenced them and to investigate whether these factors were related to frailty.
Results
Three factors were found, each relating to a different set of variables: F1 representing comorbidities; F2 being an aggregated of disturbances in physical activity, cognitive status and anaemia; and F3 portraying alterations of the emotional sphere. The concordance study showed a strong association of F2 with frailty: adjusted OR 3.65 (95% CI 1.57 to 8.53). F3 presented a milder relationship: OR 2.54 (95% CI 1.28 to 5.02). No association of F1 with frailty was found: OR 1.15 (95% CI 0.58 to 2.26).
Conclusions
In our quest to characterise frailty, we found that this is best described by an aggregate of reduction in physical activity, impairment in cognitive status and anaemia, while comorbidities are not associated to it. This could support the phenotypic model against other paradigms.
Fecal microbiome predicts treatment response after the initiation of semaglutide or empagliflozin uptake
Authors: Klemets Annabel , Reppo Ingrid , Krigul Kertu Liis , Volke Vallo , Aasmets Oliver , Org Elin 
Published: September 2024

Abstract

The gut microbiome has been shown to be affected by the use of many human-targeted medications, and the interaction can be bidirectional. This has been clearly demonstrated for type 2 diabetes medications that have been in clinical use for several decades. However, the bidirectional effects of novel type 2 diabetes drugs semaglutide, empagliflozin, and the gut microbiome have yet to be clearly described. Considering this, we investigate the effect of semaglutide and empagliflozin initiation on the gut microbiome of type 2 diabetes patients. In addition, we analyze whether the pre-treatment gut microbiome can predict the treatment efficacy.
In the study, patients with type 2 diabetes donated gut microbiome fecal samples at four timepoints (Baseline, Month 1, Month 3; Month 12) that were studied using 16S ribosomal RNA gene sequencing and analysis. Subjects additionally donated plasma and urine samples for quantitative measurement of clinical markers before treatment initiation and at Months 3 and 12. Repeated measures ANOVA paired with paired t-tests were used to analyze the effects of drug initiation on the gut microbiome. Pearson correlation was used to identify microbial features associated with the change in clinical parameters.
First, semaglutide and empagliflozin use is associated with changes in the gut microbiome after treatment initiation, but changes in microbial diversity were not detected. Moreover, the baseline gut microbiome predicted changes in glycohemoglobin for semaglutide and empagliflozin users.
Based on the results, our findings suggest that semaglutide and empagliflozin impact the gut microbial community during treatment. In addition, the baseline gut microbiome can predict semaglutide treatment effects.
Adiabatic Compressibility of Different Water Samples With Temperature Variations (281 K to 303k) – a Pilot Study
Authors: Ghildyal Divya , Chaudhary Manisha 
Published: September 2024

Abstract

The present work highlights the study of effect of temperature variation between (281–303 K) on ultrasonic velocity and adiabatic compressibility for three different water samples, tap water (TDS = 1060), RO water (TDS = 74) and distilled water (TDS = 06). Other factors analysed along with temperature variation and TDS (Total Dissolved Salts) were density and refractive index. All three water samples showed variations from usual trend in specific temperature ranges. Ultrasonic velocity showed a minor increase in tap water between (285–288K), instead of decreasing, similarly for RO water ultrasonic velocity varied as per comparison with literature review in the range (291–294K), and for distilled water ultrasonic velocity showed small variations between (286–287K). Density of the three water samples under study, showed a uniform decline with increase in temperature, highest value was observed for tap water 1000.716 kg/m3 and lowest for distilled water 995.706kg/m3. Regression Statistics yielded positive results for correlation between TDS and adiabatic compressibility for Tap water. Further studies on compressibility of water with varying salts, could pave way for new applications in hydraulics and shock absorption equipment’s.
Are Novice Resident Physicians Capable of Executing Freehand Cognitive Fusion Transperineal Prostate Biopsies ?
Authors: Guo Yize , Li Bin , Yan Yongchao , Ding Xuemei , Li Yanjiang , Zhang Wei 
Published: September 2024

Abstract

Background
The study aimed to evaluate the differences in prostate cancer (PCa) detection rates between novice and experienced resident physicians using free-hand cognitive fusion transperineal prostate biopsy (fTP-Bx) through propensity score matching (PSM).
Methods
A retrospective analysis was conducted on a cohort of patients who underwent fTP-Bx procedures. The needle biopsies were performed by two groups of surgical doctors with varying levels of prostate biopsy experience (Novice Group and Experienced Group) between March 1, 2023, and March 1, 2024. The PSM method was employed to compare the differences in cancer detection-related parameters between various groups of differing prostate biopsy experience levels.
Results
In total, 398 patients were included in the study, with 196 in the Experienced Group and 202 in the Novice Group. Prior to PSM, significant differences were observed between the groups in terms of operation duration (p = 0.014) and multiparametric MRI results (mpMRI, p = 0.009). However, after adjusting for confounding factors through PSM, there were no differences in the absolute number of cores involved, percentage of cores involved, clinically significant prostate cancer (csPCa) detection rates, and overall PCa detection rates between the different prostate biopsy experience groups. Despite potential variations in operation duration related to different levels of needle biopsy experience, there were no distinctions observed between novice and experienced doctors in terms of prostate cancer detection, particularly concerning csPCa.
Conclusions
Novice resident physicians have the ability to complete qualified fTP-Bx.
Optical Extreme Learning Machine classifier using speckles with engineered statistics
Authors: Barboza Allan , de Paula Amanda , Oriá Marcos , Chevrollier Martine 
Published: September 2024

Abstract

The growing need for fast and efficient processing by Machine Learning algorithms in the face of the exponential increase in data in various areas of activity has driven the search for innovative solutions. A promising approach is the use of light, to take advantage of increased parallelism, large bandwidths and low energy consumption inherent to optical methods. In this context, we present here a hybrid optical-electronic classifier that employs speckles designed to generate on-demand intensity distributions, following normal or non-normal statistics as needed. Using the Extreme Learning Machine technique, the system is trained to recognize image properties encoded in speckle patterns. In numerical simulations as well as in experiments, we observed a significant variation in image classification accuracy when transitioning from normal to non-normal regime. The results show satisfactory performance of the system for handwritten digit recognition.
Usefulness of Hepatic CT Attenuation Values in Assessment of Fontan-Associated Liver Disease Progression: A Comparative Study with MELD-XI Scores
Authors: Kugo Yosuke , Kido Takashi , Watanabe Takuji , Hasegawa Moyu , Nagashima Toshiaki , Taira Masaki , Yoshioka Daisuke , Shimamura Kazuo , Miyagawa Shigeru 
Published: September 2024

Abstract

Background
Fontan-associated liver disease (FALD) leads to liver cirrhosis and carcinoma, worsening patient prognosis. While the Model of End-Stage Liver Disease Excluding INR (MELD-XI) score correlates with FALD severity, precise prediction methods remain elusive. The hepatic computed tomography (CT) attenuation is used to assess steatohepatitis, which may offer insight into FALD progression. This study aimed to determine the usefulness of hepatic CT attenuation values in assessing FALD progression.
Methods
All data of post-Fontan patients at our institution between 1980 and 2021 were reviewed. Patients with postoperative abdominal CT data followed up for > 20 years postoperatively were included. We set five regions of interest with a 20-mm-diameter circle in the peripheral region of the liver to obtain minimum, maximum, mean, and standard deviation (SD) of the liver CT attenuation values. The values were indexed by that of the aorta (aVMIN, aVMAX, and aVMEAN). The impact of liver CT attenuation values on cirrhosis development diagnosed by liver biopsy was evaluated.
Results
In total, 30 patients were included, 9 of whom developed cirrhosis. In the Cox regression model, significantly associated factors for cirrhosis were aVMIN, aVMAX, SD, and MELD-XI scores. aVMIN and SD were correlated with concomitant MELD-XI scores. In cirrhosis patients, aVMIN and SD showed longitudinal alteration before cirrhosis, while MELD-XI scores remained stable.
Conclusion
The hepatic CT attenuation values were associated with cirrhosis development. Changes in the hepatic CT attenuation are more sensitive to FALD progression than the MELD-XI score.
The impact of kidney disease on peri-operative outcomes of novel BPH surgeries across the board: A propensity score matched analysis using the ACS-NSQIP database
Authors: Nasrallah Oussama G. , Herrera Maya T. , Heidar Nassib F. Abou , Mahdi Jana H. , Nasr Rami W. 
Published: September 2024

Abstract

Background:
To assess the effect of novel surgical techniques (Laser Enucleation of the Prostate (LEP) and Laser Vaporization of the Prostate (LVP)) compared to Transurethral Resection of Prostate (TURP), on post-operative outcomes stratified based on kidney function.
Methods:
The ACS-NSQIP database was reviewed from 2008-2021 for 83020 patients that underwent TURP, LEP, and LVP. Pre-operative variables were compared across kidney function groups: G1(normal/high function); G2-G3(mild/moderate kidney disease); and G4-G5(severe kidney disease). Multivariate logistic regression was performed on 30-day peri-operative complications adjusting for pre-operative variables. Propensity score matching was done between LEP and LVP to TURP.
Results:
In the G1 category, LEP showed significant protective effect against UTI (OR=0.59[0.41-0.84]), shorter hospital stay (OR= 0.72[0.60-0.87]), and longer operative time (OR=5.29[4.50-6.22]). LVP had a significant protective effect against bleeding (OR=0.19[0.11-0.37]), shorter hospital stay (OR=0.18[0.16-0.20]), and decreased return to OR (OR=0.59[0.45-0.78]).
In the G2-G3 category, LEP showed significant protective effect against UTI (OR=0.65[0.50-0.83]), sepsis (OR=0.48[0.26-0.88]), shorter hospital stay (OR=0.64[0.56-0.71]), and longer operative time (OR=5.47[4.90-6.09]). LVP had significant protective effect against bleeding (OR=0.35[0.27-0.45]), shorter hospital stay (OR=0.24[0.23-0.26]), decreased return to OR (OR=0.71[0.60-0.85]), thromboembolic events (OR=0.64[0.42-0.97]), and shorter operative time (OR=0.95[0.90-0.99]).
In the G4-G5 category, LEP lost the protective effect against UTI and sepsis and had longer operative time (OR=5.29[3.02-9.26]). LVP had significant protective effect against cardiac complications (OR=0.27[0.07-0.97]), bleeding (OR=0.49[0.28-0.85]), and shorter hospital stay (OR=0.30[0.24-0.39]).
Conclusion:
LEP and LVP are superior to TURP in terms of post-operative complications. The type of endoscopic surgery technique should be tailored according to patient’s pre-operative kidney status and risk.
Comparison of W14 fastening system variants depending on clamping force
Authors: Chudyba Łukasz , Lisowicz Tomasz , Piech Piotr , Stanisławski Przemysław 
Published: September 2024

Abstract

This paper presents a comparative analysis of the impact of individual elements of the W14 fastening system on clamping force. The results of laboratory tests carried out in accordance with the applicable European standards of the PN-EN 13481 and PN-EN 13146 series are described synthetically.
Microangiopathy in temporal lobe epilepsy with diffusion MRI alterations and cognitive decline
Authors: Liu Joan , Binding Lawrence , Puntambekar Isha , Patodia Smriti , Lim Yau , Mrzyglod Alicja , Xiao Fenglai , Pan Shengning , Mito Remika , deTisi Jane , Duncan John S , Baxendale Sallie , Koepp Matthias , Thom Maria 
Published: September 2024

Abstract

White matter microvascular alterations in temporal lobe epilepsy (TLE) can influence local hemodynamics and are relevant to understanding acquired neurodegenerative processes and cognitive impairment associated with this condition. We quantified microvascular changes, myelin, axonal and glial/matrix labelling in the gyral core and deep temporal lobe white matter regions in surgical resections from 44 TLE patients with or without hippocampal sclerosis. We compared this pathology data with in-vivo MRI diffusion measurements in co-registered regions and neuropsychological measures of pre-operative cognitive impairment and decline. We observed increased arteriolosclerosis in TLE compared to controls (greater sclerotic index, p < 0.001) which was independent of age. Microvascular changes included increased vascular densities in some regions but uniformly reduced mean vascular size (COL4, p < 0.05 to 0.0001), and increased pericyte coverage of small vessels and capillaries particularly in deep white matter (PDGFRβ and SMA, p < 0.01) which was more marked the longer the epilepsy (p < 0.05). We noted increased glial numbers (Olig2, Iba1) but reduced myelin (MAG, PLP) in TLE compared to controls, particularly prominent in deep white matter. Gene expression analysis showed a greater reduction of myelination genes in HS than non-HS cases and with age and correlated with diffusion MRI alterations. Glial densities and vascular size were increased with increased MRI diffusivity and vascular density with Fixel-Based analysis white matter regions. Increased perivascular space associated with reduced fractional anisotropy as well as age-accelerated cognitive decline prior to surgery (p < 0.05). In summary, likely acquired microangiopathic changes in TLE, including vascular sclerosis, increased pericyte coverage and reduced small vessel size, may indicate a functional alteration in contractility of small vessels and haemodynamics that could impact on tissue perfusion. These morphological features are detectable in-vivo on white matter diffusion MRI and might explain cognitive decline in TLE.
Clinical efficacy of non-eugenol containing periodontal dressing with and without platelet-rich fibrin on wound healing after surgical gingival depigmentation: a randomized clinical trial
Authors: Hesham Aya , Nawawy Marwa , Moussa Mahmoud , Darhous Mona 
Published: September 2024

Abstract

Aim: The study aimed to evaluate the effect of platelet-rich fibrin on wound healing after surgical depigmentation
Methodology: Twenty-four patients with gingival pigmentation were recruited and randomly assigned to the control group; non-eugenol periodontal dressing only, or to the test group; L-PRF with periodontal dressing. Gingival depigmentation was done using a combination of scalpel and bur techniques and both groups were covered using periodontal dressing. The healing index, epithelization test and pigmentation recurrence were tested and compared.
Results: The healing index in the test group was 91.7% of the patients scored 4 (Very good) on the 5th day, while in the control group, 83.3% scored 2 (poor) on the 5th day and 16.7% were observed to have a score 3 (Good). The epithelization test was the most significant with 100% receiving a negative result in the test group after 5 days with 66.7% completely healed and 33.3% partially healed when compared to 100% of the control group having a positive score (no epithelization). The pigmentation recurrence after 6 months was observed in 66.7% in the test group while only 33.3% in the control group.
Conclusion: Using PRF after gingival depigmentation results in better wound healing when compared to using periodontal packs alone.
Facile and versatile fabrication of multifunctional cotton fabrics with persistent antibacterial and deodorizing properties
Authors: Han Hongwei , Hu Jiangtao , Zhou Jialiang , Gu Jiawei , He Xiang , Gao Qianhong , Wu Guozhong 
Published: September 2024

Abstract

Endowing cotton fabrics with enduring antibacterial and deodorizing functions while maintaining their original hygroscopic properties is a significant challenge. In this study, a novel surface-modified cotton fabric (Cot-g-PAA/PHMG) was prepared through irradiation-induced graft polymerization of acrylic acid (AA), followed by electrostatic adsorption of polyguanidine salt (PHMG). The influence of PAA/PHMG loading on the structure and overall performance of Cot-g-PAA/PHMG was thoroughly investigated and compared with the original cotton fabric. The results showed that the PAA/PHMG layer significantly enhances the antibacterial and deodorizing properties of the cotton fabric. Specifically, Cot-g-PAA/PHMG can absorb both acidic and alkaline gaseous pollutants due to its amphoteric structure, achieving removal rates of 95.3% for ammonia and 98.4% for acetic acid, after 50 rounds of accelerated washing, the deodorization rate of Cot-g-PAA/PHMG remains above 90%. Additionally, the guanidine-functionalized cotton fabrics exhibit antibacterial rates of up to 99.99% against Gram-negative Escherichia coli and 99.99% against Gram-positive Staphylococcus aureus, even after 50 laundering cycles. These findings provide a simple and versatile approach for creating antimicrobial and odor-resistant materials for hygienic applications.
A study on the efficacy of Group PM+ on depressed mood in medical students
Authors: Zhang Bing , Ji Sifan , Cui Yuqiu , Guo Yang , Jiang Cheng , Yang Tingting , Yu Fengqiong , He Kongliang 
Published: September 2024

Abstract

Objective：The prevalence of mental health problems among medical students is higher than that of students in other disciplines and continues to increase, which may pose a risk to medical students in their subsequent studies and careers. This study adopts a Group Problem Manager Plus (PM+) intervention with medical students and investigates its effectiveness. The study demonstrates the effectiveness of Group PM+ at treating depressed mood and mental health illnesses, providing an important theoretical and experimental basis for new therapies to improve the physical and mental health of medical students.
Methods: (1) Participants: Fifty-two participants were recruited through online distribution of the scale and randomly divided into an experimental group (received 5 weekly sessions of the Group PM+ intervention) and a control group (sharing psychology knowledge online only) after interviewing each participant individually. (2) Scales: The participants in both groups completed pre- and post-tests that included the Patient Health Questionnaire-9 (PHQ-9), General Anxiety Disorder-7 (GAD-7), UCLA Loneliness Scale, Toronto Alexithymia Scale (TAS), and Chinese version of the Interpersonal Reactivity Index (IRI-C).
Results: (1) The results of the repeated measures ANOVA showed a significant improvement in depressed mood in Group PM+ (F1,43 = 10.460, P = 0.002) and significantly improved anxiety (F1,43 = 19.007, P < 0.001). (2) The Group PM+ intervention also diminished feelings of loneliness due to depressed mood and partially improved alexithymia.
Conclusions: (1) Group PM+ interventions can effectively treat depression and anxiety in medical students, alleviate feelings of loneliness, and reduce alexithymia in this population.