bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dnd1-mediated epigenetic control of teratoma formation in mouse
Authors: Gu Wei , Mochizuki Kentaro , Otsuka Kei , Hamada Ryohei , Takehara Asuka , Matsui Yasuhisa 
Published: January 2018

ABSTRACT
Spontaneous testicular teratoma develops from primordial germ cells (PGCs) in embryos; however, the molecular mechanisms underlying teratoma formation are not fully understood. Mutation of the dead-end 1 (Dnd1) gene, which encodes an RNA-binding protein, drastically enhances teratoma formation in the 129/Sv mouse strain. To elucidate the mechanism of Dnd1 mutation-induced teratoma formation, we focused on histone H3 lysine 27 (H3K27) trimethylation (me3), and found that the levels of H3K27me3 and its responsible methyltransferase, enhancer of zeste homolog 2 (Ezh2), were decreased in the teratoma-forming cells of Dnd1 mutant embryos. We also showed that Dnd1 suppressed miR-26a-mediated inhibition of Ezh2 expression, and that Dnd1 deficiency resulted in decreased H3K27me3 of a cell-cycle regulator gene, Ccnd1. In addition, Ezh2 expression or Ccnd1 deficiency repressed the reprogramming of PGCs into pluripotent stem cells, which mimicked the conversion of embryonic germ cells into teratoma-forming cells. These results revealed an epigenetic molecular linkage between Dnd1 and the suppression of testicular teratoma formation.
The utility of transcriptomics in fish conservation
Authors: Connon Richard E. , Jeffries Ken M. , Komoroske Lisa M. , Todgham Anne E. , Fangue Nann A. 
Published: January 2018

ABSTRACT
There is growing recognition of the need to understand the mechanisms underlying organismal resilience (i.e. tolerance, acclimatization) to environmental change to support the conservation management of sensitive and economically important species. Here, we discuss how functional genomics can be used in conservation biology to provide a cellular-level understanding of organismal responses to environmental conditions. In particular, the integration of transcriptomics with physiological and ecological research is increasingly playing an important role in identifying functional physiological thresholds predictive of compensatory responses and detrimental outcomes, transforming the way we can study issues in conservation biology. Notably, with technological advances in RNA sequencing, transcriptome-wide approaches can now be applied to species where no prior genomic sequence information is available to develop species-specific tools and investigate sublethal impacts that can contribute to population declines over generations and undermine prospects for long-term conservation success. Here, we examine the use of transcriptomics as a means of determining organismal responses to environmental stressors and use key study examples of conservation concern in fishes to highlight the added value of transcriptome-wide data to the identification of functional response pathways. Finally, we discuss the gaps between the core science and policy frameworks and how thresholds identified through transcriptomic evaluations provide evidence that can be more readily used by resource managers.
Exercise Effects on Adipose Tissue Postprandial Lipolysis and Blood Flow in Children
Authors: YAN HUIMIN , PIERCE JOSEPH R. , MYERS KIMBERLY B. , DUBOSE KATRINA D. , DUBIS GABRIEL S. , TANNER CHARLES J. , HICKNER ROBERT C. 
Published: January 2018

ABSTRACT
Poor suppression of lipolysis and blunted increase in blood flow after meal ingestion in obese adults may indicate resistance to the antilipolytic action of insulin. Exercise may be used to normalize lipolytic responses to food intake by increasing insulin sensitivity.

Purpose
To determine if acute bouts of aerobic exercise and/or excise training alter lipolytic and blood flow responses to food intake in lean (LN) and obese (OB) children.

Methods
Sixty-five children (9–11 yr) were randomized into acute exercise (EX: 16 LN and 28 OB) or control (CON: 9 LN and 12 OB) groups that exercised (EX), or rested (CON) between standardized breakfast and lunch. Microdialysis probes were inserted into the subcutaneous abdominal adipose tissue to monitor interstitial glycerol (lipolysis) and blood flow. Changes in interstitial glycerol and nutritive flow were calculated from dialysate samples before and after each meal. A subgroup (OB = 15 and LN = 9) from the acute exercise group underwent 16 wk of aerobic exercise training.

Results
Poor suppression of lipolysis and a blunted increase in adipose tissue nutritive blood flow in response to breakfast was associated with BMI percentile (r = 0.3, P < 0.05). These responses were normalized at lunch in the OB in the EX (P < 0.05), but not in OB in the CON. Sixteen weeks of exercise training did not improve meal-induced blood flow and marginally altered the antilipolytic response to the two meals (P = 0.06).

Conclusions
Daily bouts of acute aerobic exercise should be used to improve the antilipolytic and nutritive blood flow response to a subsequent meal in obese children.
Video-Recorded Validation of Wearable Step Counters under Free-living Conditions
Authors: TOTH LINDSAY P. , PARK SUSAN , SPRINGER CARY M. , FEYERABEND MCKENZIE D. , STEEVES JEREMY A. , BASSETT DAVID R. 
Published: January 2018

ABSTRACT

Purpose
The purpose of this study was to determine the accuracy of 14-step counting methods under free-living conditions.

Methods
Twelve adults (mean ± SD age, 35 ± 13 yr) wore a chest harness that held a GoPro camera pointed down at the feet during all waking hours for 1 d. The GoPro continuously recorded video of all steps taken throughout the day. Simultaneously, participants wore two StepWatch (SW) devices on each ankle (all programmed with different settings), one activPAL on each thigh, four devices at the waist (Fitbit Zip, Yamax Digi-Walker SW-200, New Lifestyles NL-2000, and ActiGraph GT9X (AG)), and two devices on the dominant and nondominant wrists (Fitbit Charge and AG). The GoPro videos were downloaded to a computer and researchers counted steps using a hand tally device, which served as the criterion method.

Results
The SW devices recorded between 95.3% and 102.8% of actual steps taken throughout the day (P > 0.05). Eleven step counting methods estimated less than 100% of actual steps; Fitbit Zip, Yamax Digi-Walker SW-200, and AG with the moving average vector magnitude algorithm on both wrists recorded 71% to 91% of steps (P > 0.05), whereas the activPAL, New Lifestyles NL-2000, and AG (without low-frequency extension (no-LFE), moving average vector magnitude) worn on the hip, and Fitbit Charge recorded 69% to 84% of steps (P < 0.05). Five methods estimated more than 100% of actual steps; AG (no-LFE) on both wrists recorded 109% to 122% of steps (P > 0.05), whereas the AG (LFE) on both wrists and the hip recorded 128% to 220% of steps (P < 0.05).

Conclusions
Across all waking hours of 1 d, step counts differ between devices. The SW, regardless of settings, was the most accurate method of counting steps.
Kostis Kornetis, Eirini Kotsovili and Nikolaos Papadogiannis, eds. Consumption and Gender in Southern Europe since the Long 1960s
Authors: Gomez-del-Moral Alejandro Jose 
Published: January 2018

Kostis Kornetis, Eirini Kotsovili, and Nikolaos Papadogiannis, eds. Consumption and Gender in Southern Europe since the Long 1960s. London: Bloomsbury Academic, 2016. xvi + 280 pp.
Patterns and Predictors of Short‐Term Peripherally Inserted Central Catheter Use: A Multicenter Prospective Cohort Study
Authors: Paje David , Conlon Anna , Kaatz Scott , Swaminathan Lakshmi , Boldenow Tanya , Bernstein Steven J. , Flanders Scott A. , Chopra Vineet 
Published: January 2018

BACKGROUNDThe guidelines for peripherally inserted central catheters (PICCs) recommend avoiding insertion if the anticipated duration of use is ≤5 days. However, short‐term PICC use is common in hospitals. We sought to identify patient, provider, and device characteristics and the clinical outcomes associated with short‐term PICCs.METHODSBetween January 2014 and June 2016, trained abstractors at 52 Michigan Hospital Medicine Safety (HMS) Consortium sites collected data from medical records of adults that received PICCs during hospitalization. Patients were prospectively followed until PICC removal, death, or 70 days after insertion. Multivariable logistic regression models were fit to identify factors associated with short‐term PICCs, defined as dwell time of ≤5 days. Complications associated with short‐term use, including major (eg, venous thromboembolism [VTE] or central line‐associated bloodstream infection [CLABSI]) or minor (eg, catheter occlusion, tip migration) events were assessed.RESULTSOf the 15,397 PICCs placed, 3902 (25.3%) had a dwell time of ≤5 days. Most (95.5%) short‐term PICCs were removed during hospitalization. Compared to PICCs placed for >5 days, variables associated with short‐term PICCs included difficult venous access (odds ratio [OR], 1.54; 95% confidence interval [CI], 1.40‐1.69), multilumen devices (OR, 1.53; 95% CI, 1.39‐1.69), and teaching hospitals (OR, 1.25; 95% CI, 1.04‐1.52). Among those with short‐term PICCs, 374 (9.6%) experienced a complication, including 99 (2.5%) experiencing VTE and 17 (0.4%) experiencing CLABSI events. The most common minor complications were catheter occlusion (4%) and tip migration (2.2%).CONCLUSIONShort‐term use of PICCs is common and associated with patient, provider, and device factors. As PICC placement, even for brief periods, is associated with complications, efforts targeted at factors underlying such use appear necessary.
Qualidade de vida e depressão entre pacientes carentes em tratamento oncológico na Casa do Amor em Vitória da Conquista BA
Authors: Lima Luciana Gonçalves , Santos Stifanny Brenda Alves dos , Gomes Gisele Norberto Mattos , Gomes Leilane Trindade , Freitas Valéria Pires , Caetité Diego Andrade , Duarte Stênio Fernando Pimentel 
Published: January 2018

A Oncologia está voltada para a forma como o câncer se evolui no organismo e qual o tratamento mais apropriado para cada tipo de neoplasia. De acordo com a Organização Mundial da Saúde (OMS), o câncer é a segunda causa de morte em países desenvolvidos, perdendo apenas para doenças cardiovasculares. Em termos estatísticos, podemos assegurar que o câncer é a doença crônica mais tratável no momento, cerca de 50% dos casos são curados. No Brasil estima-se que este número seja mais baixo, devido ao fato de que os diagnósticos são feitos tardiamente. As causas de câncer são diversas, podendo ser externas, internas ou inter-relacionadas. Mesmo depois dos progressos com o tratamento para o câncer e as chances de sobrevivência, cada vez maiores, o câncer ainda está correlacionado como uma doença que provoca dor, sofrimento e morte iminente (KUBLER-ROSS, 1981), fazendo com que assim o diagnostico tenha um grande impacto psicológico no individuo, resultando em ansiedade; angustia; duvidas; ódio; desesperança; medo entre outros. Segundo Minayo, Hartz e Buss (2000), “qualidade de vida tem sido ligada ao grau de satisfação encontrado na vida familiar, amorosa, social e ambiental e à própria estética existencial”. Foi realizado um estudo transversal, quantitativo com o objetivo de avaliar a qualidade de vida e depressão dos pacientes oncológicos em tratamento na Casa do Amor em vitória da Conquista. Foram entrevistados 30 pacientes, 10 mulheres e 20 homens ambos diagnosticados câncer.
Risk factors of long-term postoperative renal function after partial nephrectomy in a solitary kidney
Authors: Zhu Jie , Kuru Timur , Wei Yingqi , Hatiboglu Gencay , Popeneciu Valentin , Schöenberg Gita , Simpfendörfer Tobias , Zeier Martin , Xin Ma , Zhang Xu , Hohenfellner Markus , Pahernik Sascha 
Published: January 2018

AbstractThe effect of warm ischemia time (WIT) on longterm renal function after partial nephrectomy remains controversial. In this retrospectively cohort study, 75 solitary kidney patients were included and the effects of warm ischemia time, preoperative renal function and resected normal parenchyma volume on long-term renal function were evaluated. Multivariable analysis showed that the preoperative renal function baseline was significantly associated with renal function 12 months postoperation (P=0.01), adjusting for age and comorbidities factors. Meanwhile, perioperative acute renal failure (ARF) events significantly affected postoperative renal function at postoperative time points of 12 months (P=0.001) and 60 months (P=0.03), as well as renal function change at postoperative 12 months (P<0.01). Warm ischemia time and resected normal parenchyma volume were not risk factors for long-term postoperative renal function, while the latter was significantly associated with renal function change (P=0.03 at 12 months, P<0.01 at 36 and 60 months).In conclusion, the quality of preoperative kidney primarily determines long-term postoperative renal function, while the quantity of preserved functional parenchyma volume was the main determinant for long-term kidney recovery. ARF was an independent risk factor while WIT was indirectly associated with postoperative renal function by causing perioperative ARF.
A pragmatic bottom-up approach to harmonize the units of clinical chemistry tests among Belgian clinical laboratories, focusing on immunoassays
Authors: Demarteau Marianne , Cammaert Piet , Vandevelde Nathalie M. , Callewaert Nico , Coucke Wim , China Bernard , Verstraete Alain G. 
Published: January 2018

Abstract

Background:
Harmonization of units is an important step to improve the comparability of clinical chemistry results, but few examples exist of successful harmonization efforts. We present the results of a pragmatic approach that was implemented in Belgium from 2012.

Methods:
After a large consultation and information of stakeholders, preferred units were proposed for 140 assays, including the 23 immunoassays discussed in more detail here. The change occurred in two phases, first involving assays for which there was no change in the numerical result, then changes involving a change in numerical results. Laboratories were invited to participate in this harmonization on a voluntary basis. The project was based on a bottom-up approach, large consultation and the pragmatic choice of the proposed units, including conventional and SI units.

Results:
The large heterogeneity of units was drastically reduced; adoption of the preferred units increased from 3% (insulin) – 45% (HCG) to 70% (insulin) – 96% (LH and FSH). Adoption of the preferred units was higher if it involved no change in numerical values (90%) than when there was a change (76%).

Conclusions:
We believe that the harmonization effort has reached its goals. Without aiming at implementing SI units for all parameters, our strategy was successful with a large majority of the laboratories switching to the proposed units. Moreover, the harmonization program is still progressing, with additional laboratories converting to the consensus units.
Beyoncé’s Slay Trick: The Performance of Black Camp and its Intersectional Politics
Authors: Chatzipapatheodoridis Constantine 
Published: January 2018

Abstract
This article pays attention to African-American artist Beyonce Knowles and her performance of black camp. Beyonce’s stage persona and performances invite multiple ideological readings as to what pertains to her interpretation of gender, sexuality, and race. While cultural theory around the icon of Beyonce has focused on her feminist and racial politics as well as her politicization of the black female body, a queer reading applied from the perspective of camp performance will concentrate on the artist’s queer appeal and, most importantly, on her exposition of black camp, an intersection of feminist, racial and queer poetics. By examining video and live performances, the scope of this article is to underline those queer nuances inherent in Beyonce’s dramatisation of black femininity and the cultural pool she draws from for its effective staging. More specifically, since Beyonce plays with tropes and themes that are common in camp culture, her performance relies on a meta-camping effect that interacts with African-American queer culture. This article, thus, traces black queer traditions and discourses in the artist’s praxis of black camp.
Love is the Message: Barkley Hendricks’s MFSB Portrait Aesthetics
Authors: Hyacinthe Genevieve 
Published: January 2018

Abstract
Barkley L. Hendricks’s (1945-2017) portraiture is considered in relation to camp’s liminal nature. Hendricks employs the camp techniques of surface emphasis, and affective audience engagement, to relay a political message of black agency. His portraiture is framed with dynamics of MFSB, a popular culture movement developed in his hometown, Philadelphia, during the era of the 1970s. The paper brings to light Hendricks’s participation within the complex histories of imaging the black body. His participation defies the hegemonies inherent within these histories and, at the same time, reifies them.
Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark
Authors: Ojo Oluwafemi Adeleke , Ojo Adebola Busola , Ajiboye Basiru Olaitan , Oyinloye Babatunji Emmanuel , Akinyemi Ayodele Jacob , Okesola Mary Abiola , Boligon Aline Augusti , de Campos Marli Matiko Anraku 
Published: January 2018

Abstract

Background:

Irvingia gabonensis stem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis. This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts from I. gabonensis (Aubry-Lecomte ex O‘Rorke) Baill bark.

Methods:
Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+ chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability. Inhibitory activities on AChE and BChE were evaluated.

Results:
The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin). The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+ to Fe2+) (5.94 mg ascorbic acid equivalent/100 g). Extracts of I. gabonensis inhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.

Conclusions:
Hence, possible mechanism through which the stem bark executes their anti-Alzheimer’s disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.
Enhanced Performances of SAC-OCDMA System by Using Polarization Encoding
Authors: Garadi Ahmed , Bouazza Boubakar S. , Bouarfa Abdelkader , Meddah Khansae 
Published: January 2018

Abstract
This article presents a novel encoder technique of spectral amplitude coded multiple access (SAC-OCDMA) systems. The proposed technique is based on two orthogonal polarization states of the same one dimensional optical code. This method is usually applied to increase the number of simultaneous users in a network, and it can double the number of potential users against the one-dimensional code. The results obtained in this work show that optical zero cross-correlation code can accommodate more users simultaneously for the typical bit error rate of optical communication system of

${\ }{10^{ - 9}}$
.
Tunable High Performance 16-Channel Demultiplexer on 2D Photonic Crystal Ring Resonator Operating at Telecom Wavelengths
Authors: Naghizade S. , Sattari-Esfahlan S. M. 
Published: January 2018

AbstractHere, we proposed a high performance 16-channel optical demultiplexer using two-dimensional photonic crystal ring resonator for telecommunication systems. By plane wave expansion (PWE) method the photonic band gap (PBG) of proposed structure calculated. Then, with finite difference time domain (FDTD) method the performance parameters of designed two-dimensional photonic crystal demultiplexer are analyzed. It is found that the channel wavelength of wavelength-division multiplexing (WDM) is truly tuned by changing the structure parameters of the demultiplexer and position of rod. Output peaks located in the optical communication C-band and L-band with the transmission efficiency of 99 %. The demultiplexer exhibits high-quality factor of 5176, and spectral width of 0.3. Very low crosstalk values are between −19 dB and −90 dB where, device only occupies an area of 1708.65 µm2. The proposed compact 16-channel demultiplexer can find more applications for the ultra-compact WDM systems in highly integrated telecommunication circuits.
Segmental Myocardial Displacement and Tissue Velocity Analysis of the Right Ventricle in Hypoplastic Left Heart Syndrome Fetuses and Controls Using Color Tissue Doppler Imaging (C-TDI)
Authors: Graupner Oliver , Enzensberger Christian , Wieg Larissa , Wolter Aline , Yerebakan Can , Khalil Markus , Axt-Fliedner Roland 
Published: January 2018

AbstractIntroduction: The long-term outcome of patients with HLHS (hypoplastic left heart syndrome) is mainly determined by right ventricular function. Our study examines, whether there are differences in segmental right ventricular myocardial displacement and tissue velocities of fetuses with HLHS compared to healthy fetuses during gestation. Materials and methods: A prospective study was conducted including 20 fetuses with HLHS and 20 gestational age matched controls. c-TDI (colour tissue Doppler imaging) derived systolic and diastolic velocities as well as myocardial displacement were assessed in three different locations of the right ventricle (RV). A ROI (region of interest) was placed in the basal, middle and apical part of the myocardium. Possible changes of c-TDI indices in the course of pregnancy and between the three different segments were investigated in both groups. Results: HLHS fetuses showed significantly lower e' velocities measured in the basal and middle part of the RV compared to healthy controls (p < 0.05). Basal displacement showed significantly lower values in HLHS fetuses compared to controls. In control fetuses but not in HLHS fetuses there was a significant increase of basal diastolic velocities and displacement in the course of pregnancy. According to myocardial velocities and displacement values there was a significant decrease from the base of the fetal heart to the apex pointed in both groups. Conclusions: An altered right ventricular myocardial function in HLHS fetuses within different myocardial segments could be demonstrated. An apicobasal gradient with higher velocity and displacement values in the basal part of RV myocardium could be found in both groups. The technique may be of value in the prenatal assessment of myocardial function, however its role as a monitoring tool and outcome predictor needs to be defined.