bluuebunny/crossref_metadata_2025_split huggingface dataset:
How do taurine and ergothioneine additives improve the parameters of high- and low-quality turkey semen during liquid preservation?
Authors: Orzołek Aleksandra , Rafalska Katarzyna Teresa , Dziekońska Anna , Rafalska Agata Monika , Zawadzka Małgorzata 
Published: September 2024

Abstract
The study aimed to examine the parameters of turkey ejaculates (n=40) of high and low quality (HQ and LQ, respectively), preserved with the addition of taurine (TAU; 5 mM, 10 mM) and ergothioneine (EGT; 5 mM, 10 mM) for 48 h at 5°C in a liquid state. The motility, plasma membrane integrity (PMI), mitochondrial function, apoptotic and necrotic spermatozoa percentage, and sperm cells that generated NO were determined after 2, 24, and 48 h of storage. The preserved semen was also analysed for the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione (GSH) content, and malondialdehyde (MDA) production. Taurine, in both doses, may improve the antioxidant status of stored turkey semen as well as sperm motility, viability and functionality regardless of ejaculate quality, as manifested by increased SOD and CAT activities, reduced MDA levels, and enhanced sperm characteristics i.e. plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), total motility (TMOT), and progressive motility (PMOT). In turn, the addition of EGT increased GSH content in the external environment and suppressed lipid peroxidation in turkey spermatozoa, in particular those from low-quality (LQ) ejaculates. However, it appears that EGT could deliver more satisfactory results when added at lower concentrations.
The Use of Moringa Leaves Extract in Rabbit Diets: Its Effect on Performance, Lipid Profile, Kidney and Liver Function, Immunity, Antioxidant, Digestive Enzymes, and Cecal Microbiota
Authors: Reda Fayiz M. , Alagawany Mahmoud , Mahmoud Hemat K. , Al-Marakby Khaled M. , Ismail Tamer Ahmed , Elnesr Shaaban 
Published: September 2024

Abstract
This study evaluated the role of Moringa oleifera leaves extract (MOLEx) in improving the performance and health of rabbits during the fattening period. The growth, carcass measurements, serum immunity, lipid profile, liver and kidney functions, digestive enzymes, antioxidant condition, cecal microbiota of rabbits have been examined. A total of 100 New Zealand White male rabbits (5-week-old) were randomly distributed into five groups and were fed on the basal diet only or the basal diet supplemented with graded levels of MOLEx (1, 2, 3 or 4 g/kg diet) for 8 weeks. Animals in each group were divided into 10 replicates, with two animals each. Results showed that dietary supplementation of MOLEx at levels of 1, 3 and 4 g/kg feed improved growth performance (P<0.05) including LBW (7, 9, 11, 13 weeks), BWG (5–13 weeks) and FCR (11–13 and 5–13 weeks). The inclusion of MOLEx in rabbits’ feed (3 and 4 g/kg) increased carcass %, dressing % and spleen % and decreased duodenum pH and cecal pH. Abdominal fat % was significantly lowered after the treatment with MOLEx. Lipid profile, liver and kidney functions, and cecal microbiota (increasing beneficial bacteria and reducing harmful bacteria) of growing rabbits were positively affected by MOLEx levels. The dietary supplementation of MOLEx improved most of antioxidant biomarkers of growing rabbits. The dietary supplementation of MOLEx (2, 3 and 4 g/kg) increased immunological indicators (IgM, IgG, IgA, lysozyme and complement 3), and lowered cortisol level compared with the control group. Digestive enzymes (protease, lipase and amylase) activities of rabbits were positively affected by MOLEx levels (3 and 4 g/kg feed). In conclusion, the inclusion of MOLEx in rabbits feed can be effective in improving productive performance, kidney and liver functions, digestive enzymes, antioxidant biomarkers, immunological indicators, cecal microbiota and carcass traits.
Comparison of FGF-8, FGF-10, FGF-Receptor 2, Androgen Receptor, Estrogen Receptor-A and SS in Healthy and Hypospadiac Children
Authors: Emaratpardaz N , Turkyilmaz Z , Karabulut R , Dayanir D , Kaya C , Sert AAE , Arkan G , Ucaner FA , Kapisiz A , Eryilmaz S , Atan A , Sonmez K 
Published: September 2024

Abstract
In this study, we aimed to investigate the levels of Fibroblast Growth Factor-8 (FGF-8), FGF-10, FGF-Receptor-2 (FGFR-2), Androgen receptor (AR), Estrogen receptor alpha and beta (ER-α and ER-β) in the foreskins of children with and without hypospadias.

Methods
Samples from the foreskins of 20 children with hypospadias and 20 skin samples from children without hypospadias between the ages of 14 months and 12 years were taken during circumcision or hypospadias correction surgery for immunohistochemical (IHC) examination of these markers. In IHC examination, it was shown that ER-α, ER-β and AR receptors were more involved in the foreskin of children with hypospadias than in the fore-skin of without hypospadias children, and FGF-8, FGF-10 and FGFR-2 were lower (p<0.05). ER and AR uptake were higher in hypospadias tissue samples and FGF-8, FGF-10, and FGFR-2 uptakes were lower compared to without hypospadias children’s tissue samples, and these factors were supported by affecting each other in the development of hypospadias. The limited number of studies on this subject in the literature and the contradictory results of the findings indicate that more research should be done on this subject in the future.
IGHV Mutational Status in a Cohort of Bulgarian CLL Patients: High Unmutated CLL Prevalence in North-East Bulgaria
Authors: Yosifova A , Micheva I , Donchev M , Tincheva S , Ormandjiev S , Genova J , Pavlova Z , Todorova A 
Published: September 2024

Abstract
Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults. One of the best established CLL prognostic markers is the somatic hypermutational status of the IGHV gene which is a part of the immunoglobulin heavy chain variable region. Technology for IGHV genotyping has been optimized and has been applied in routine diagnostics for the first time in Bulgaria. A total of 105 patients with CLL from different Bulgarian regions were tested. IGHV mutational status was determined by Sanger sequencing on total genomic DNA (gDNA) or RNA extracted from mononuclear cells. All sequencing profiles were analyzed with the IMGT/V-QUEST tool. Within the course of the analysis a high percentage of IGHV unmutated status was established in the Varna district on the Black Sea (Northeast Bulgaria). In addition, the IGHV genotyping performed on gDNA revealed a rare case with multiple rearrangements. The present data from IGHV genotyping will help in choosing the proper treatment for the benefit of Bulgarian CLL patients.
Novel DGAT1 Mutations Identified in Congenital Diarrheal Disorder 7: A Case Report with Therapeutic Experience
Authors: Shi C , Liu XL , Li XN , Zhao YJ 
Published: September 2024

Abstract
Congenital diarrheal disorders (CDD) are a group of rare inherited intestinal disorders, among which CDD7 was recently identified to be associated with only 24 mutations in gene coding for diacylglycerol-acyltransferase 1 (DGAT1).
We report on a female patient who presented with diarrhea, vomiting, hypoalbuminemia, and failure to thrive after birth. Two novel variants of c.1215_1216delAG and c.838C>T were found in the DGAT1 gene by whole exome sequencing, which was confirmed to be compound heterozygous by Sanger sequencing. Her symptoms and nutritional status improved significantly after 1 year of a fat-restricted enteral diet. Weight for age and weight for length increased from −5.0 SDS and −4.0 SDS at 3 months to +0.08 SDS and +1.75 SDS at 15 months, respectively.
This report expanded the mutation spectrum of DGAT1-related CDD7 and enriched our knowledge of the clinical features. Moreover, early fat-restricted enteral diet intervention was suggested for the treatment of such patients.
ANXA5 and VEGFA Gene Variants in Women with Early Pregnancy Losses from North Macedonia
Authors: Terzikj M , Bozhinovski Gj , Branoski A , Dimkovska M , Kubelka-Sabit K , Plaseska-Karanfilska D 
Published: September 2024

Abstract
Early pregnancy loss (EPL) is the most common pregnancy complication, found in approximately 15% of all clinically recognized pregnancy complications. Up to date, various maternal as well as fetal factors are reported as a cause of EPLs. However, in approximately 50% of EPL cases, the exact cause is not clearly identified and these cases are referred as idiopathic.
The aim of our study was to examine the association of four distinct variants in the ANXA5 gene and two variants within the VEGFA gene in a cohort of women with EPLs from North Macedonia. This group was compared to a control group of women matched by ethnic background without pregnancy loss and at least one live birth. We also aimed to establish an effective and cost-efficient method for their detection based on multiplex single-base extension.
Among 190 women experiencing EPLs, and 190 samples from women without a history of pregnancy loss (control group), our results demonstrated a statistically significant prevalence of heterozygotes for the M2/ANXA5 haplotype in women with EPLs, compared to the control group (p=0.0006). In the analyses comparing genotypic frequencies for the variants in the VEGFA gene, higher frequencies were generally observed among women experiencing EPLs, however without statistical significance.
Our study aligns with multiple studies showing that M2 and M1 ANXA5 haplotypes are more prevalent in patients with pregnancy loss and presents an affordable genotyping technique for the specific ANXA5 and VEGFA variants.
Investigation and assessment of oxidant – antioxidant status of some enzymes and vitamins in Iraqi patients with chronic myeloid leukemia
Authors: Chyad Zahraa Sabri , Matter Al-Maliki Abbas D. 
Published: September 2024

Abstract

Aim. To investigate and assess the concentrations of glutathione peroxidase, superoxide dismutase, catalase, vitamin A and vitamin C in Iraqi patients infected by chronic myeloid leukemia (CML).

Material and methods. Forty-eight Iraqi patients of ages ranged between (15-75) years took part in the current study. Enzymatic antioxidants (Gpx, SOD and CAT) and antioxidant vitamins (A and C) were estimated spectrophotometrically in blood sera of all patients according to age and sex variables, and then all clinical parameters were compared with healthy individuals (control groups).

Results. Evaluation of oxidant-antioxidant status. Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) enzymes concentrations recorded high significant decreasing (***p <0.0001) in blood serum of CML patients according to age and sex factors. Moreover, vitamins A and C showed a high significant decreasing (***p <0.0001) in their concentrations as measured in blood serum of CML patients with diverse ages and sexes. The GPx, SOD, CAT, Vitamin A and Vitamin C in the current study, are considered excellent biochemical markers for following the acuity of CML disease depending on age and sex variables.

Conclusion. The clinical significance of oxidative stress represented by lack of enzymes and vitamins in the current study was proven. Statistically significant differences were recorded and observed between patients and control groups depending on age and sex factors.
Biopharmaceutical aspects of the development of transdermal forms of Lisinopril dihydrate
Authors: Shyteyeva Tatyana , Bezchasnyuk Elena , Kryskiv Oleg , Baranova Inna 
Published: September 2024

Abstract
Prevention and treatment of arterial hypertension is of great importance. Improvement of existing medicines through the use of alternative routes of administration can enhance the pharmacotherapeutic characteristics of active pharmaceutical ingredients (APIs). Transdermal therapeutic systems (TTS) allow for delivery of a drug through intact skin into the systemic circulation, while minimizing side effects. The development of transdermal formulations of antihypertensive drugs, Lisinopril dihydrate in particular, has practical and scientific significance. Optimization of the algorithm for the development of transdermal drugs involves in vitro preformulation studies of the membrane permeability of APIs and the identification of factors that affect this process.
The tests were performed by dialysis through a semipermeable hydrophilic membrane using a Valia-Chien diffusion device. The effect of the initial concentration of Lisinopril dihydrate on the flux rate I
s was investigated. Different donor concentrations of Lisinopril dihydrate were tested at three levels, 10, 20 and 30 mg/ml, accordingly. It was noted that the permeation process of Lisinopril dihydrate under model conditions corresponds to zero-order kinetics and is characterized by a uniform speed. The correlation coefficient (R
2) for all the kinetic equations was 0.999. A linear dependence of the flux velocity of Lisinopril dihydrate on the initial concentration was indicated. The concentration of Lisinopril dihydrate in the donor solution of 30 mg/ml was found to be optimal for further stages of pharmaceutical development of the transdermal formulation. The studies of Lisinopril dihydrate permeability allow to give a positive assessment of the acceptability of this API for use in the transdermal formulation and the development of TTS.
Faecal microbiota transplantation associated adverse events
Authors: Patryn Rafal , Kazmierczak-Wojtas Natalia , Bulas Lucyna , Boretska Olga , Szkultecka-Debek Monika , Drozd Mariola , Blicharski Tomasz 
Published: September 2024

Abstract
Faecal microbiota transplantation (FMT) aims to restore intestinal microbiota balance with the objective of normalising its composition and achieve therapeutic benefits. The procedure involves the administration of fresh or frozen faecal microbes from a healthy donor into the recipient’s gastrointestinal tract with the intent to restore the proper structure and functionality of the recipient’s intestinal microbial community. Evidence showing the positive effects of FMT is abundant, however, less attention has been devoted to FMT-associated adverse events, especially in relation to liver diseases. Based on literature review, studies and reports regarding FMT-associated adverse events since the beginning of FMT use, have been analysed. The review covering the period 2010-2022 was undertaken in accordance with the PRISMA guidelines.
Studies conducted on the patient population suffering from various types and forms of liver disease have proven the possible effectiveness of the FMT method and reported moderate adverse events (nausea, constipation, flatulence). Severe adverse events occurring in relation to FMT were also noted. No safety issues or infection signals associated with FMT were observed in studies performed within the population suffering from cirrhosis.
The present review of scientific reports, publications and literature reviews describes the adverse events reported in the literature. Faecal microbiota transplants are associated with adverse events classified as mild, moderate and severe, among others, diarrhoea, fever, infections or death. There is a need to implement a donor screening programme and personalised transplantation methods. Further research is recommended to assess and monitor FMT efficacy, benefits and risks.
IoT enabled industrial fault monitoring and prediction
Authors: Ranjan Harsh , Gudipalli Abhishek 
Published: September 2024

Abstract
Automation is now heavily used in the industry. Human effort is much reduced by implementation of an automation system that monitors and signals system faults that may arise. The number of devices that can be connected to the Internet of Things (IoT) is growing every day. With the aid of the IoT model, a hardware prototype will be developed that will automatically monitor industrial parameters such as humidity, temperature, fire, smoke, pressure, and sense motion, as well as generate alerts and alarms. Each parameter is continuously monitored using an application called Blynk. This proposed work will use IoT to provide information remotely about the current weather and gas concentrations in the plant via email. The collected information can also be used for predictive analysis. The idea behind this proposed work is to accumulate and visualize data about the various parameters utilized in industries by enabling IoT.
Revisiting ΣΤΟΡΓΗ and ΑΓΑΠΗ IN Sirach, 3 and 4 Maccabees
Authors: Chia Philip Suciadi 
Published: September 2024

Abstract
Both στοργή and ἀγάπη are widely translated as love. BDAG understands στοργή as the love of spouse: husband to his wife or wife to her husband. Αγάπη, on the other hand, generally refers to a sacrificial love revealed in Christ in the Christian literature. This research, however, refuses BDAG’s limited aspect of στοργή and a general understanding of ἀγάπη as God’s sacrificial love in Christ. This article, therefore, attempts to provide more comprehensive understandings of these two loves by revisiting στοργή and ἀγάπη in the book of Sirach, 3 and 4 Maccabees. To achieve this goal, this research employs lexical analysis for its methodology.
Kohelet, Sadducees and Pharisees
Authors: Mandey Jenry E. C. 
Published: September 2024

Abstract
According to my recent research, the book of Kohelet has a link to the emergence of the Sadducees (Mandey, 2023). As a product of a thought movement, Kohelet could be the forerunner of Sadduceism. This paper aims to give a closer look at the link between Kohelet and Sadduceism as well as examine their relationship with the Pharisees as another movement that emerged alongside the Sadducees. Despite the challenges of historical information about the emergence of both the Sadducees and the Pharisees, a socio-historical analysis is conducted using literary sources.
Synthesis and Structural Study on the Cd(II)-Gd(III) Mixed-Metal Complex Based on 2,6-pyridinedicarbonyl bis(N,N-diethylthiourea)
Authors: Thu Ha Nguyen , Huy Nguyen Hung 
Published: September 2024

The mixed-metal complex with the composition of [GdCd2(L)3](PF6) (H2L = 2,6-pyridinedicarbonyl bis(N,N-diethylthiourea) has been synthesized by the ‘one-pot’ reaction of GdCl3∙6H2O, Cd(CH3OO)2∙2H2O and H2L with 1 : 2 : 3 molar ratio in methanolic solution. The structure of the obtained complex is characterized by IR spectroscopy, mass spectrometry and single-crystal X-ray diffraction. The results reveal a cationic trinuclear mixed-metal complex composed of one Gd(III) cation, two Cd(II) cations and three dianionic ligands {L2–}. The charge of the complex is compensated by a PF6– anion.
Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico
Authors: Bare Yohanes , Sari Dewi Ratih Tirto , Mogi Marsiana Coo , Nurak Maria Marcelina Dua 
Published: September 2024

<p class="E-JOURNALAbstractBodyEnglish">Brown algae is a marine functional food that high fibers, vitamins, and bioactive compounds. Brown algae improved blood glucose and lipid profile. This study investigated the potential activity of two bioactive compounds from brown algae, as anti-obesity through pancreatic lipase inhibition. Molecular docking was conducted for investigating the lipase inhibition of bioactive compounds. Lipase structure (PDB ID 1ETH) was obtained from protein data bank and docked with fucodiphlorethol and phloroglucinol in specific position. Then, the complex was analyzed by PyMol following by Discovery Studio version 21.1.1. Phloroglucinol - pancreatic lipase showed five amino acid residues by hydrogen bonds, hydrophobic and electrostatic. Fucodiphlorethol - pancreatic lipase generated binding energy -392 kJ/mol and consisted of eight hydrogen bonds, three electrostatic, and 4 hydrophobic interactions. The active sites of fucodiphlorethol and phloroglucinol with lipase were not considered as active sites or catalytic sites. Therefore, we predicted that both of fucodiphlorethol and phloroglucinol inhibit lipase allosterically by altering the protein structure conformations. molecular docking analysis suggested that fucodiphlorethol and phloroglucinol have potential antiobesity effect by inhibiting lipase. Molecular dynamic are needed for further investigation.</p>
Effect of diet-induced obesity and non-drug options of its correction on the functional and morphological liver characteristics in female Wistar rats
Authors: Basalai A. A. , Mityukova T. A. , Kuznetsova T. E. , Chudilovskaya K. N. , Poluliakh O. Y. , Kastsiuchenka M. S. , Shcherbakov Y. V. , Hubkin S. V. 
Published: September 2024

A nonalcoholic fatty liver disease is one of the unfavorable consequences of obesity. However, experimental studies on the mechanisms of this pathology development are being carried out mainly on male rodents, and the data on the development of fatty hepatosis in females are insufficient.The aim of the study was to investigate the effect of diet-induced obesity and non-drug options for its correction on the morphological liver characteristics and liver metabolic parameters in female Wistar rats. Experiments were conducted on sexually mature female Wistar rats and included the study of morphological and biochemical parameters of the liver functional state in animals kept on standard diet (StD, 16 weeks), on a high-calorie diet (HCD, 16 weeks) when switching to a standard diet (HCD/StD, 8/8 weeks), when the physical activity in the form of treadmill running was included (StD + running, HCD + running and HCD/StD + running, 8/8 weeks).Long-term use of HCD in female rats caused visceral obesity, liver fatty dystrophy with disruption of organ histoarchitectonics, shifts in hepatic metabolism and increased lipid peroxidation. Correction of diet-induced obesity by switching to StD led to a complete or partial normalization of the studied indices. In the “HCD + running” group, metabolic disorders are often more pronounced than in the “HCD” group. The transition to HCD/StD + running promoted the most complete restoration of metabolism and histostructure of the liver with regeneration signs of the organ.Thus, an optimal correction of visceral obesity, fatty liver dystrophy and its metabolism is impossible without diet normalization in female Wistar rats. An additional regenerating effect with respect to the liver histostructure is achieved with a combined variant of correction ‒ transition to a balanced diet and moderate physical activity.
Non-invasive assessment of the dynamics of change in the vascular bed in the buccal autograph during treatment of extended urethral strictures
Authors: Strotsky A. V. , Obrazkov K. O. , Yasiukaits D. R. 
Published: September 2024

The male urethral stricture disease treatment despite all successful achievements and treatment options still remains one of the most actual and complex problems of modern urology. The presence of prolonged urethral strictures complicated with such factors as expressed scared deformation, infection, fistulae, previous unsuccessful urethral interventions, total urethral obliteration require the use of two- or multistage operations. In the light of recent times, the improvement methods dedicated to buccal mucosa graft adaptation assessment and autotransplant microvascularity evaluation are very relevant nowadays. In this study we made an attempt to estimate the dermatoscopy role in objective assessment of buccal mucosa graft microvascular network after two staged substitutive urethroplasty.The analysis of graft microphotos made in different time periods after urethroplasty allows us to define the main thing. In our point of view, the absence of evaluation changes after 90–120 postoperative days allows us to estimate a correct time for the second stage of urethroplasty-tubularization. stages of microvasculature evolution.Considering the statement that microvasculature evolution is the evolution of its graft, we think that the correct research direction is to study microangiogenesis stimulation methods.