bluuebunny/crossref_metadata_2025_split huggingface dataset:
JAG2: A Potential Biomarker for Microtia Identified by Integrated RNA Transcriptome Analysis
Authors: Wu Xu , Fu Yaoyao , Ma Jing , Li Chenlong , Zhang Tianyu , He Aijuan 
Published: September 2024

Introduction:
Microtia, a prevalent congenital maxillofacial deformity, significantly impacts
the physical and psychological health of children. Its etiology, especially in non-syndromic
cases, remains a complex and partially understood domain, complicating etiological treatment. Recent
studies pointed to a genetic predisposition in non-syndromic microtia, yet research on susceptible
or pathogenic genes is limited.

Objective:
This study focused on identifying key biomarker genes in microtia cartilage to elucidate
pathogenesis and assist in prenatal diagnosis.

Methods:
We first collated two bulk transcriptome datasets from the GEO database, followed by
functional enrichment analysis and Weighted Gene Co-expression Network Analysis (WGCNA) to
pinpoint differentially expressed genes (DEGs) and gene modules. The subsequent intersection of
DEGs with WGCNA modules, aided by support vector machine-recursive feature elimination
(SVM-RFE) and protein-protein interaction (PPI) networks, predicted potential susceptibility genes
for microtia. Finally, we integrated bulk RNA sequencing with single-cell data via the “scissor” R
package and further validated it with Real-time PCR and immunofluorescence.

Results:
We identified JAG2 as a prominent biomarker for microtia, evidenced by its significant upregulation
in microtia cartilage.

result:
we identified JAG2 as a prominent biomarker for microtia, evidenced by its significant upregulation in microtia cartilage, further validated by RT-PCR and immunofluorescence

Conclusion:
Our findings implicate JAG2 in microtia development and suggest its role in chondrocyte
maturation and differentiation through Notch signaling pathway activation, shedding light on
the potential pathogenesis of microtia.
Osteoarthritis: Insights into Potential Causes and Biomarkers from Articular Fluid Metabonomics
Authors: Xu Hongyao , Zhou Yang , Pan Zhu , Gao Zengxin 
Published: September 2024

Background:
Osteoarthritis (OA) is one of the leading causes of pain and disability.
Metabolomics is a recent approach to identifying moieties that can be used in diagnosis, prognosis,
and as biomarkers. The articular fluid in OA witnesses its pathophysiology directly. Identifying
differential metabolites and biological pathways associated with them can provide in-depth
mechanisms and identify biomarkers.

Aim:
In the present study, synovial fluid of confirmed OA patients (n=17) and matched healthy
controls (n=21) was investigated for differential metabolites using untargeted metabolomics.

Methods:
The distribution of the samples was observed using multidimensional PCA. The signaling
pathways associated with metabolites were obtained by pathway enrichment analysis, and a
correlation analysis of differential metabolites was performed to identify common and significant
metabolites.

Results:
A total of 16 upregulated and 23 downregulated metabolites (log2foldchange >0.5,
p<0.05) were identified by differential analysis. The essential amino acid, arginine, was recognized
as the most significant metabolite present in most of the identified deregulated pathways.
The pathways, arginine, and proline metabolisms, and mTOR, were found to be deregulated
strongly in the present study.

Conclusion:
These metabolites and their associated pathways can be beneficial for the diagnosis
and treatment of osteoarthritis in clinical settings after further validation in large cohorts.
Molecular Analysis of PICK1 Mediating TLR4 in Testicular Cells
Authors: Shi Chun , Cao Zi Yu , Yang Ming Yuan , Zhai Mei Jing 
Published: September 2024

Background:
Rodent pests cause significant damage to the national economy and human
health in China. Rodent infertility control technology offers a new and less toxic approach to managing rodent pests. The use of a plant complex sterility agent has shown the potential to induce male
infertility, but the molecular mechanism behind this effect remains unclear and requires further investigation.

Methods:
Sixty Sprague Dawley (SD) rats were randomly assigned to control, low-dose (10 mg/kg
of plant complex sterility agent containing shikonin and acetylstilbestrol), and high-dose (20 mg/kg
of plant complex sterility agent) groups. The rats received intragastric administration of the agent for
30 days. Sperm count and testosterone levels were assessed to evaluate fertility. The protein interacting with C kinase 1 (PICK1) and Toll-like receptor 4 (TLR4) levels in the control group, low-dose
group, and high-dose group were measured by immunohistochemistry (IHC) and Western Blotting
(WB).

Results:
In comparison to the control group, both the sperm concentration (p < 0.05) and the levels
of testosterone (p < 0.05), PICK1 (P < 0.05), and TLR4 (p < 0.05) were significantly decreased in a
dose-dependent manner in the low dose and high dose groups. These changes were confirmed
through microscopy, ELISA, IHC, and WB.

Conclusion:
The plant complex sterility agent demonstrates potential in reducing sperm concentration and down-regulating testosterone levels. It likely exerts its effects by impairing male fertility in
rats through the inhibition of PICK1 and TLR4 protein levels
Evaluation of Mode and Psychiatric Co-morbidity of Intentional Self Harm: A Hospital Based Study.
Authors: Shrestha Rajesh , Lamichhane Ram , Sharma Bhaskker 
Published: September 2024

Introduction: Intentional self harm is a potentially self injurious action with a non-fatal outcome for which there is evidence, either explicit or implicit, that the individual intended to kill himself or herself. Suicide is a fatal act of self-injury (self harm) undertaken with more or less conscious self destructive intent. There has been an increase in the number of patients presenting with attempted suicide in the emergency settings of hospitals. This study aimed to explore socio-demographic characteristics, pattern of attempts, psychiatric diagnosis, psychosocial and personality factors among survivors of suicide attempt presenting to a tertiary care hospital. Methods: The study included 100 survivors of intentional self harm registered over a period of six months visiting a tertiary care hospital. The cases directly presented to Psychiatry department and were referred from medical and other departments for psychiatric evaluation. They underwent evaluation by consultant psychiatrist and received appropriate interventions. Results: Majority of the victims were female (73%), belonging to the age group of 20-40 years. The most common method of attempt was self poisoning with pesticides (65%) followed by hanging (16%) and overdose of drugs (7%). Most of the attempts (68%) were impulsive in nature. Mental illness was diagnosed in (65%) of the cases, mainly depressive disorder (51%), and personality disorder (10 %). Most of the attempts (67%) were triggered by psycho-social factors. Conclusion: The pattern shows predominance of female gender, young age group, a role of mental illness, impulsivity and psychosocial factors in intentional self harm.
Online Medical Education in Nepal: Barking a Wrong Tree
Authors: Nepal Binu , Atreya Alok 
Published: September 2024

To combat the global pandemic of Covid-19, the Government of Nepal declared a country wide lockdown on 24 March, 2020. The lockdown affected all the institutions, medical schools being no exception.
To keep in pace with the academic calendar, most of the universities instructed their affiliated medical schools to start online classes for medical undergraduates. Kathmandu University which affiliates ten medical schools under its umbrella circulated a notice to start online classes. To facilitate this, the most important requisite is internet connection. A working device in the form of a laptop, desktop, or smartphone is needed to log in through the internet. After the lockdown, when all the medical schools closed, the students went home. We should not forget the fact that many students are from rural locality with no access to the internet at their home. The mobile internet data is costly and more data is consumed during video conferencing in online classes. The geographical remoteness further hinders the network coverage across the country.
India Amidst COVID-19 Crisis: The Good, The Bad and The Ugly
Authors: Negi Sanjay Singh 
Published: September 2024

Beating the statistics: With a population of over a billion, high population density with overcrowding especially in metropolis and underdeveloped health infrastructure, the projections for corona virus disease (COVID-19) pandemic were not in favour of India. Most statistical models had predicted over 5 lakh cases and more than 38,000 deaths and warned that India must prepare for a tsunami of COVID-19 cases. The situation appeared to be grimmer given that the incidence of diabetes, hypertension and respiratory diseases due to tuberculosis andair pollution is higher amongst Indians which are known risk factors for severe disease and death due to COVID-19.
Continuous Glucose Monitoring With Geriatric Principles in Older Adults With Type 1 Diabetes and Hypoglycemia: A Randomized Controlled
Trial
Authors: Munshi Medha N. , Slyne Christine , Adam Atif , Conery Colin , Oladunjoye Adeolu , Neuwahl Simon , Wypij David , Toschi Elena 
Published: September 2024

OBJECTIVE
Continuous glucose monitoring (CGM) use in older adults with type 1 diabetes (T1D) has shown benefits. However, the impact of CGM use, coupled with simplified treatment regimens and personalized glycemic goals that are better suited for older patients with multiple comorbidities and hypoglycemia, is not known.

RESEARCH DESIGN AND METHODS
Older adults (≥65 years) with T1D with hypoglycemia (two or more episodes of hypoglycemia [blood glucose &lt;70 mg/dL for ≥20 min over 2 weeks]) who were CGM naïve or CGM users were randomized to intervention and control groups. The intervention consisted of the combined use of CGM with geriatric principles (i.e., adjusting goals based on overall health, and simplification of regimens based on CGM patterns and clinical characteristics) over 6 months. The control group received usual care by their endocrinologist. The primary end point was change in time when blood glucose was &lt;70 mg/dL from baseline to 6 months. Cost-effectiveness was calculated using a health care sector perspective.

RESULTS
We randomized 131 participants (aged 71 ± 5 years; 21% ≥75 years old) to the intervention (n = 68; CGM users = 33) or the control (n = 63; CGM users = 40) group. The median change in hypoglycemia from baseline to 6 months was −2·6% in the intervention group and −0.3% in the control group (median difference, −2.3% [95% CI −3.7%, −1.3%]; P &lt; 0.001). This improvement was seen in both CGM naïve (−2.8%; 95% CI −5.6%, −0.8%) and CGM users (−1.2%; 95% CI −2.7%, −0.1%). The HbA1c did not differ between the groups (7.5% vs 7.3%). The intervention was cost-effective (incremental cost-effectiveness ratio $71,623 per quality adjusted life-year).

CONCLUSIONS
In older adults with T1D and high risk of hypoglycemia, CGM use enhanced by geriatric principles can lower hypoglycemia without worsening glycemic control in a cost-effective fashion.
ensino religioso chegou na Base Nacional Comum Curricular (BNCC)
Authors: Freitas Eliane Maura Littig Milhomem de , Amorim Cleyde 
Published: September 2024

Este artigo problematiza os resultados da pesquisa pós-doutoral realizada em 2022/23, cujo objetivo geral consistiu em identificar as práticas pedagógicas dos/as professores/as do Ensino Religioso (ER) no município de Cariacica - ES, para verificar se a BNCC contribui para a docência da disciplina na perspectiva não confessional. A pesquisa teve como aporte teórico-metodológico discussões como a de Gatti et all (2019), sobre formação de professores, de Freitas (2018), sobre a visibilidade da disciplina, de Junqueira (2020) sobre o ensino religioso na BNCC, além das diretrizes e ordenamento legal a respeito do tema. A metodologia utilizada foi a da pesquisa exploratória, que contou com entrevistas semiestruturadas a professores/as da rede de ensino, como também a pesquisa bibliográfica que sustentou parte das discussões apresentadas. Como resultados da pesquisa destacamos a dificuldade que os/as docentes demonstraram em lecionar sobre a diversidade religiosa tanto pela formação acadêmica considerada insatisfatória para o que determina a disciplina, como também a dificuldade em falar sobre as religiões minoritárias sobretudo as de matrizes africanas tanto por desconhecimento como pelo racismo estrutural.

Palavras-chaves:
Ensino Religioso no ES. Ensino religioso e BNCC. Formação Continuada e Ensino Religioso. Ensino Religioso Não Confessional. Religiões Afro-brasileiras.
Quest: Mulheres na ciência e os elementos químicos
Authors: Da Silva Beatriz Fernanda , Londero Buttchewits Karol , Bulegon Brondani Patrícia , Costa Nogueira Keysy Solange 
Published: September 2024

Resumo
Neste artigo é relatado o desenvolvimento e validação de um jogo que possibilita a discussão acerca do apagamento de mulheres e cientistas e suas contribuições para a construção da Tabela Periódica (TP). Tratar do tema mulheres na ciência é importante e necessário, visto que vivemos em uma sociedade fundamentada no patriarcado, que minimiza os feitos femininos e, se apropria deles. Ao longo da história, observa-se o esquecimento de diversas mulheres e cientistas, que contribuíram de forma significativa para a identificação de elementos químicos, características físico-químicas dos elementos, identificação de isótopos, entre outros. Este artigo busca destacar a relevância da contribuição dessas mulheres através de um jogo interativo tipo QUEST. O jogo didático aborda em suas categorias a história e contribuições de mulheres cientistas e também conteúdos da TP: sua organização, propriedades, aplicabilidade dos elementos químicos. O jogo foi validado com estudantes de licenciatura e bacharelado em Química e mostrou-se uma ferramenta eficaz de ensino.

Palavras-chave: Tabela periódica, ensino de química, jogo didático.
Abstract
In this article, the development and validation of a game that enables the discussion about the erasure of women scientists and their contributions to the construction of the Periodic Table (PT) are reported. Addressing the topic of women in science is important and necessary, given that we live in a society grounded in patriarchy, which minimizes female achievements and appropriates them. Throughout history, the forgetting of various women scientists is observed, who have made significant contributions to the identification of chemical elements, physical-chemical characteristics of elements, identification of isotopes, among others. This article seeks to highlight the relevance of the contribution of these women through an interactive QUEST-type game. The educational game covers in its categories the history and contributions of women scientists and also content related to the PT: its organization, properties, and the applicability of chemical elements. The game was validated with undergraduate students in Chemistry and proved to be an effective teaching tool.
Keywords: Periodic Table, chemistry education, educational game.
História da Ciência e a formação de professores: uma revisão de literatura
Authors: Ventura Mariana , Quintiliano de Carvalho Isabel , De Faria Fernanda Luiza 
Published: September 2024

RESUMO

Desde o século XX o estudo da História da Ciência para a educação em ciência vem sendo discutido. Os pesquisadores dessa temática defendem as abordagens históricas como meio de contextualizar e humanizar o ensino sobre ciências. Dessa forma, o objetivo desse trabalho foi mapear as produções realizadas sobre a temática História da Ciência no que tange o Ensino de Química com foco na formação de professores (as) nas edições de 2010 a 2020 do ENEQ, 2011 a 2021 do ENPEC e de 2010 a 2020 do BDTD. O método escolhido foi o levantamento bibliográfico a partir de palavras chaves como História da Ciência, formação inicial de professores e História da Química. Ao todo foram encontradas 43 publicações. Os trabalhos foram analisados a partir da Análise de Conteúdo, da qual emergiram cinco categorias: 1) Programa de Formação Docente; 2) Formação inicial de professores; 3) Formação continuada de professores; 4) Concepções de professores em exercício; 5) Análise curricular. Para o ENPEC, se acrescentou uma sexta categoria, a revisão bibliográfica. Os resultados apontam que o uso da História da Ciência em sala de aula ainda é pouco discutido e que se faz extremamente necessário a inclusão da HC na formação de professores.

PALAVRAS-CHAVE: História da Ciência, Ensino de Química, Revisão Sistemática, Formação docente.

ABSTRACT

Since the 20th century, the study of the History of Science for science education has been discussed. Researchers on this subject defend historical approaches as a means of contextualizing and humanizing science teaching. The aim of this study was therefore to map productions on the subject of the History of Science in relation to Chemistry Teaching, with a focus on teacher training, in the 2010-2020 editions of ENEQ, 2011-2021 editions of ENPEC and 2010-2020 editions of BDTD. The method chosen was a bibliographic survey using key words such as History of Science, initial teacher training and History of Chemistry. A total of 43 publications were found. The papers were analyzed using Content Analysis, from which five categories emerged: 1) Teacher Training Program; 2) Initial teacher training; 3) Continuing teacher training; 4) Conceptions of practicing teachers; 5) Curriculum analysis. For the ENPEC, a sixth category was added, the literature review. The results show that the use of History of Science in the classroom is still little discussed and that it is extremely necessary to include History of Science in teacher training.

KEYWORDS: History of Science, Chemistry Teaching, Systematic Review, Teacher Training.
REDES SOCIAIS E CHOQUE DE OPINIÕES
Authors: Aparecida Paula da Costa Braga Marilene , Tinoco Cabral Ana Lúcia 
Published: September 2024

Este trabalho tem por objetivo apresentar o desenvolvimento de uma atividade direcionada para estudantes do segundo ano do Ensino Médio cujo foco é o uso de estratégias de polidez para gerenciar a divergência de opiniões nas redes sociais. Buscando uma reflexão acerca da polidez linguística como um valor cultural importante nas interações sociais e na vivência cidadã, a base teórica é da Pragmática, especificamente, os estudos da polidez. As análises focalizam a identificação de impolidez por parte dos alunos e a sugestão de estratégias de polidez. Os resultados mostram que a atenuação estritamente pragmática e a cortesia positiva foram as estratégias mais comumente empregadas pelos estudantes para a construção de um ambiente virtual harmonioso frente aos pontos de vista, por vezes, antagônicos presentes nas interações das redes sociais.
Pendidikan Karakter Siswa SDN 15 Tampe melalui Pengalaman Spiritual Kristiani
Authors: Vasantan Priska , Suseno Agustinus Bayu 
Published: September 2024

Manusia yang berkualitas memiliki intelektualitas dan integritas yang baik. Peningkatan intelektualitas dapat dilakukan melalui proses pembelajaran, sedangkan pembentukan integritas dipengaruhi oleh karakter yang baik. Pembentukan karakter seseorang dimulai sejak dini. Siswa SD adalah salah satu benih bangsa yang senantiasa perlu dipupuk dan dibentuk menjadi pribadi yang berguna bagi bangsa dan negara. Pengabdian masyarakat di SDN 15, Bengkayang ini adalah membentuk karakter siswa SD melalui proses pembelajaran Bahasa Indonesia dan pengalaman spiritual kristiani di daerah perbatasan. Daerah perbatasan memiliki permasalahan yang cukup unik dibandingkan dengan daerah lainnya, seperti perbedaan budaya, pendidikan yang minim, dan lain sebagainya. Pendampingan ini diberikan selama 4 minggu, meliputi pedidikan karakter di kelas Bahasa Indonesia, konseling dan rekoleksi siswa. Dalam kelas Bahasa Indonesia ini, 6 mahasiswa PGSD memberikan pendidikan karakter melalui nasehat, kesopanan dalam kelas, dll. Bimbingan dan konseling diberikan oleh 5 mahasiswa PGSD kepada 5 siswa yang dipandang perlu mendapatkan pendampingan konseling, sedangkan rekoleksi diberikan sebagai penutup pendampingan. Untuk mengakhiri pengabdian ini, dilakukan evaluasi bersama pihak sekolah SDN 15. Hasil evaluasi pada akhir pengabdian masyarakat ini adalah perlunya pendampingan lanjutan dan rekoleksi bagi guru dan orang tua siswa.
Comparative Analysis of Phytochemical Composition and Biological Activities of Sprouted and Unsprouted Chenopodium quinoa Willd. Seeds: Insights Into Nutritional Value and Functional Properties
Authors: Chouikh Atef , Ben Ali Anis , Bousbia Brahim Aida , Khezzani Khaoula , Bekkouche Saadia 
Published: September 2024

Abstract
Our study explored the phytochemical composition and biological properties of sprouted and unsprouted quinoa seed extracts (red, black, and yellow varieties). We observed distinct differences in primary metabolites between sprouted and unsprouted seeds, particularly in carbohydrates, fats, and proteins. Sprouted seeds exhibited increased levels of simple soluble sugars, amino acids, and fatty acids. Additionally, sprouted seeds showed higher quantities of polyphenols and flavonoids, with the black variety displaying the most. However, antioxidant activity did not directly correlate with phenol and flavonoid content, with unsprouted seeds demonstrating superior performance. Conversely, unsprouted seed extracts displayed greater anti-inflammatory efficacy, attributed to saponin loss during germination. Specifically, the best carbohydrate value was found in sprouted red seeds at 21.55±1.07 mg/g of plant material, the highest protein content was in sprouted red seeds at 4.6±0.28 mg/g, and the best lipid content was in sprouted black seeds at 2.97±0.13 mg/g. The highest polyphenol content was in sprouted black seeds at 10.74±0.6 mg E GA/g Ex, while the highest flavonoid content was also in sprouted black seeds. The most effective antioxidant activity in the DPPH test was observed in non-sprouted black seeds with an IC50 of 0.06±0.01 mg/ml. The highest hemolysis inhibition was found in sprouted red seeds at 49.04% at 0.8 mg/ml, and the greatest anti-inflammatory activity was in non-sprouted yellow seeds at 1.6±0.07 mg E Dc/mg Ex.
Effect of Blanching Methods and Probiotic Bacteria on the Bioactive Compounds and Physicochemical Parameters of Fermented Brown Agaricus bisporus and Imleria badia Mushrooms
Authors: Venugopal Kavya , Bernaś Emilia 
Published: September 2024

Abstract
The brown A. bisporus - a cultivated species and I. badia – a wild species of mushroom containing health-promoting properties has been selected to create functional foods. Therefore, the study analyzed the effect of the blanching method, fermentation temperature and addition of probiotic bacteria on the course of lactic fermentation and changes in mushrooms quality. The mushroom caps were blanched in water for 30 s and 2 min, followed by microwave-blanching for 2 min. To increase the effectiveness of fermentation and bioactivity of the product a starter culture of Lactobacillus acidophilus strain LA-5 (Hansen) was added to the brine. The mushrooms were fermented at 21°C and 26°C. In both the species fermentation concluded within 3 days with pH < 4.5. The blanching and the addition of probiotic bacteria significantly affected bioactive compounds, compared to fermentation temperature. Concerning the mushroom’s quality, blanching was necessary before fermentation. Products that were water-blanched for 2 min displayed increased dietary fiber and glucans content, while products from A. bisporus microwave-blanched gave organoleptic properties. The obtained products had significant amounts of B vitamins and phenols, proving that fermentation benefited the retention and enhancement of the nutritional quality of mushrooms. Fermented mushrooms can be used in nutraceuticals.