bluuebunny/crossref_metadata_2025_split huggingface dataset:
381 Effects of L-glutamate and L-aspartate supplementation on intestinal immunity and intestinal barrier integrity of weaned piglets challenged with F18 ETEC
Authors: Wongchanla Supatirada , Park Sangwoo , Kim Kwangwook , Sun Shuhan , Li Xunde , Liu Yanhong 
Published: September 2024

Abstract
L-glutamate (Glu) and L-aspartate (Asp) are pivotal components in the cellular metabolic pathways and immune regulation of pigs. This study aimed to investigate the effects of Glu and Asp on systemic and intestinal immune responses of weaned piglets challenged with an enterotoxigenic Escherichia coli (ETEC) F18. Weaned pigs [n = 49; 8.18 ± 1.54 kg body weight (BW)] were randomly assigned to one of seven treatments, with seven piglets per treatment. The treatments included negative control (NC) and positive control (PC) that were fed with control diet without or with ETEC challenge, respectively. The other five dietary treatments were supplemented with either 1% or 2% Glu or Asp, or 50 mg/kg of Carbadox, and were challenged with ETEC. All pigs except for NC were challenged with F18 ETEC orally for three consecutive days at the dose of 1010 CFUּ dose-1ּ d-1. The study period lasted for 3 wk, with a 7-d adaptation period and 14 d post-inoculation (PI). On d 14 PI, jejunal and ileal mucosa were collected to analyze the mRNA expression of inflammatory cytokines and tight junction proteins by quantitative polymerase chain reaction. All data were analyzed with ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Among all treatments, pigs fed with carbadox showed greatest (P &lt; 0.05) mRNA expression of interleukin (IL)10 in jejunal mucosa. Supplementing 2% Asp or carbadox had less (P &lt; 0.05) expression of IL6 than PC in jejunal mucosa, while supplementing 1% Asp had lowest (P &lt; 0.05) expression of IL6 in ileal mucosa. Pigs fed with 1% Glu had greatest (P &lt; 0.05) expression of IL17A and IL22, while pigs fed with 2% Glu had greatest (P &lt; 0.05) expression of IL23 among all treatments in jejunal mucosa. However, dietary treatments did not affect mRNA expression of IL17A, IL22, and IL23 among all treatments in ileal mucosa. Compared with PC, supplementing 1% Asp had greater (P &lt; 0.05) expression of MUC2, OCDN, and ZO1 in ileal mucosa. Supplementation of 2% Glu or 1% Asp had greater (P &lt; 0.05) expression of MUC2 in jejunal mucosa than NC. Among all treatments, pigs fed with 2% Asp showed greatest (P &lt; 0.05) expression of CLDN1 in ileal mucosa, while showed least (P &lt; 0.05) expression of CLDN1 in jejunal mucosa. However, no difference was observed in expression of OCDN and ZO1 in jejunal mucosa. In conclusion, results of the present study indicate that dietary supplementation of Glu or Asp affected both intestinal immunity and integrity of ETEC-challenged weaned pigs with similar trends as the supplementation of carbadox.
441 Development of a multi-objective feed formulation to improve sustainability of swine production
Authors: De Quelen Francine , Labussiere Etienne , Wilfart Aurélie , Dourmad Jean-Yves , Garcia-Launay Florence 
Published: September 2024

Abstract
Traditional feed formulation focuses on minimizing cost while meeting nutritional requirements and does not consider the environmental impacts associated with producing feed ingredients. Incorporating the impacts of feed ingredients into the formulation process has been proposed as a way to mitigate the impacts of pig production. Garcia-Launay et al. (2018) developed a multi-objective (MO) formulation method based on the environmental impacts of feed ingredients calculated by Life Cycle Assessment (LCA). The objective of this study was to quantify the effects of such eco-friendly pig diets on nitrogen (N) and energy (E) balances, the components of heat production (HP) and the performance of finishing pigs. We compared two different diets: a control diet in accordance with practices in French commercial farms (Control-diet) and an eco-diet formulated with the MO method (Eco-diet). The Eco-diet contained reduced proportion of cereals (50% vs 70%), no oil meals (0% vs 8%) and greater proportions of coproducts (19.5% vs 5%) and protein crops (26% vs 10%) in comparison with the Control-diet. Pietrain x (Large White x Landrace) entire males [n = 12; mean body weight (BW) = 81 kg] were used in this study (n = 6 per experimental diet). Animals were housed individually in digestible cages equipped for measurement of individual feed and water intake, feeding behavior, and separate collection of feces and urine. All animals were adapted to experimental conditions, diets, and digestible cages during 14 d. After the adaptation period, pigs were housed in climate respiration chambers (one pig in an individual cage per chamber) for 6 d to measure digestibility of nutrients, energy and nitrogen balance, and heat production components calculated according to Labussière et al. (2013). We did not observe any significant effect of the dietary treatment on growth performance of pigs (feed intake, average daily gain, feed conversion ratio or water consumption). The digestibility coefficients of dry matter (DM) and N were not different between the two groups. N excreted in urine was significantly greater for the group of pigs fed the Control-diet than for the group fed the Eco-friendly diet (13.5 vs 11 gּ pig-1ּ d-1, respectively, P = 0.03) with no difference for N excreted in feces between the two groups. N retention was less in the Control-diet group in comparison with the Eco-diet group (28.3 vs 30.4 gּ pig-1ּ d-1, respectively; P = 0.05). The E balance was similar between the two groups. The protein deposition was less in the Control-diet group than the Eco-diet group (177 vs 190 g/d, respectively, P = 0.05). Formulating feeds with adequate net energy, SID amino-acids and protein levels while choosing feed ingredients with least environmental impacts, such as co-products, can be an effective way to decrease environmental impacts of pig production without consequences on animal performances.
31 Sustainable livestock, sustainable future: Why we need a toolkit of solutions to improve sustainability in animal agriculture
Authors: Mitloehner Frank 
Published: September 2024

Abstract
Meat and dairy will continue to be an essential part of diets around the world, but as demand increases so does pressure to reduce the environmental impact of farming. There is no single solution that will allow the global community to meet demand and improve sustainability in our food system. We will need a toolkit of solutions to meet the needs of producers in various regions and production systems.
105 Fetal hypothyroidism dysregulates local hepatic and renal renin-angiotensin systems in a temporal manner
Authors: Smith Alyssa A , Rudy Kaylyn , Pasternak J Alex 
Published: September 2024

Abstract
The renin-angiotensin system (RAS) is an essential endocrine system with roles in electrolyte balance, blood volume, and blood pressure. While the canonical RAS exerts systemic vasoregulatory effects through steps involving hepatic, renal, and pulmonary-derived compounds, there is also evidence of localized, independently acting RAS in organs such as the liver and kidney. Further, modulations in RAS resulting from fetal hypothyroidism have previously been documented, although the temporal specificity of this endocrine interaction is not well-characterized. Thus, the objective of the current study was to examine the time-dependency of modulations in local hepatic and renal RAS in a porcine model of fetal hypothyroidism. To achieve this, pregnant gilts (n = 24) were divided evenly into either a control (CON) or methimazole (MMI) treatment group, with the MMI group receiving 5 mgּ kg-1ּ d-1 of oral MMI treatment and the CON group receiving an equivalent sham carrier. Each group was further divided into four timepoints (n = 3/treatment at each timepoint), with treatment beginning on d 34, 45, 55, or 65 of gestation. After 21 d of treatment, and at d 55, 66, 76, and 86, respectively, the gilts were humanely euthanized, blood samples taken from all fetuses (n = 340), and thyroid, liver (LVR), and kidney (KID) collected from a subset of four fetuses per litter (n = 96). Confirmation of fetal hypothyroidism in the MMI group was accomplished via histological assessment of the thyroid and measurement of circulating thyroxine (T4) concentrations. Each MMI-treated fetus (n = 96) subset showed evidence of goitrous thyroid histology, with the absence of healthy eosinophilic colloid indicating successful disruption of the fetal hypothalamic-pituitary-thyroid axis. T4 assays performed on a similar subset of n = 96 fetuses showed that MMI treatment significantly reduced T4 concentrations within each timepoint relative to CON (P &lt; 0.001 at all timepoints). Following confirmation of hypothyroidism, RNA was extracted from the fetal LVR and KID samples to allow for gene expression analysis by qPCR. Ct values were normalized, fold changes calculated using the 2-∆∆Ct method, and differences within timepoint determined using a Wilcoxon signed-rank test. AGT, the precursor to all bioactive RAS peptides, was significantly downregulated in LVR at d 66 (P = 0.014) and 76 (P &lt; 0.001). ACE was significantly upregulated at d 76 in both LVR (P = 0.037) and KID (P = 0.005). No significant changes were observed for ACE2 in either tissue, or for renal REN. Finally, AGTR1, the receptor that mediates RAS effects, was significantly downregulated at d 55 in KID (P &lt; 0.001) and d 76 in LVR (P = 0.023), while also being upregulated at d 86 in LVR (P = 0.033). Collectively, these results show that fetal hypothyroidism modulates RAS function in a gestational age-dependent manner, which may alter fetal development and subsequent postnatal physiology.
109 Dam and calf influences on colostrum yield and quality in beef cattle
Authors: Meyer Allison M , Redifer Colby A , Rathert-Williams Abigail R 
Published: September 2024

Abstract
To determine factors affecting colostrum yield and quality, data from 148 cow-calf pairs over 6 experiments were combined. In each experiment, single rear quarter colostrum yield was obtained post-calving but pre-suckling. Colostrum IgG was determined by ELISA, and colostrum lactose, triglycerides (as a measure of total fat), and protein were determined by colorimetric analyses. Total IgG and nutrients were calculated as the concentration multiplied by single-quarter yield. Calf birth weight and expelled placental dry weight were also collected post-calving. Mixed linear models (experiment as a random effect) were used to determine the effects of dam age (primiparous: 2 yr, young: 3 to 4 yr, mature: ≥ 5 yr), dam peripartum body condition score (BCS; thin: &lt; 5, moderate: 5 to 5.99, fleshy: ≥ 6), and calf sex on colostrum yield (single-quarter colostrum weight) and quality. Pearson correlation coefficients were also determined for colostrum yield with dam pre-calving body weight (BW), calf birth weight, calf birth weight relative to dam BW, whole placental dry weight, and gestation length. Mature dams had the greatest (P ≤ 0.03) and primiparous dams had the least (P ≤ 0.001) colostrum yield and total lactose. Colostrum from primiparous dams had greater (P = 0.03) IgG concentration than mature and greater (P ≤ 0.001) protein concentration than young and mature. Mature dams had greater (P ≤ 0.02) total IgG, total protein, and total triglycerides compared with both primiparous and young. Colostrum yield was less (P ≤ 0.04) for thin than moderate and fleshy dams. Total IgG was unaffected (P = 0.11) by dam BCS; however, thin dams had greater (P ≤ 0.04) IgG concentration than moderate and fleshy. Lactose concentration and total lactose were less (P ≤ 0.02) in colostrum from thin compared with moderate dams. Protein concentration was greater (P = 0.001) in thin than moderate dams, but thin dams had less (P ≤ 0.02) total protein compared with moderate and fleshy. Dam BCS did not affect (P ≥ 0.59) colostrum triglyceride concentration or total triglycerides. Colostrum yield and quality were not affected (P ≥ 0.22) by calf sex. Pre-calving dam BW was positively correlated with colostrum yield (r = 0.46, P &lt; 0.001). Colostrum yield was also positively correlated with calf birth weight (r = 0.31, P &lt; 0.001) and whole placental dry weight (r = 0.26, P = 0.007), but tended to be negatively correlated with calf birth weight relative to dam BW (r = -0.14, P = 0.09). Gestation length had no relationship (P = 0.31) with colostrum yield. Overall, colostrum yield and quality are negatively affected in young and thin beef dams. In addition, both dam and calf weight have positive relationships with colostrum yield, likely mediated by endocrine action associated with placental size.
112 Effects of maternal body condition on colostrum, foal weight, and foal IgG concentration
Authors: Summers Cheyenne S , Tillquist Nicole M , Engle Terry E , Barnello Nicholas M , Voggu Santhi Priya , Reiter Amanda S , Govoni Kristen E , Zinn Steven A , Reed Sarah A 
Published: September 2024

Abstract
Foals are born with little to no immunity and rely on maternal colostrum to develop adequate circulating immunoglobulin G (IgG) concentrations in the periparturient period. Colostrum directly impacts the health of the foal, and there are limited data assessing how maternal metabolism and body condition impacts IgG in colostrum and in the foal postnatally. To determine the influence of maternal body condition score (BCS) and cresty neck score (CNS) on glucose and insulin metabolism in the pregnant mare, colostrum composition, and colostrum and foal IgG concentrations, light breed mares (n = 6) were divided into two groups based on BCS and CNS at 315 ± 0.45 d of gestation. The groups were identified as moderately obese (MO; n = 3, BCS) and lean (LN; n = 3). Mares were fasted overnight and then underwent an intravenous (iv) glucose tolerance test at 315 ± 0.45 d gestation. Blood samples were collected at -30, -15 and 0 min and mares were administered dextrose iv [0.3 g/kg body weight (BW)] over 3.7 ± 0.17 min followed by an intravenous infusion of insulin (0.3 mU/kg BW) 20-min later. Additional blood samples were collected 2, 5, 10, 15, 20, 25, 30, 45, 60, and 120 min after initiation of dextrose infusion. At parturition, a colostrum sample was collected before foals nursed and analyzed for protein and fat, and IgG concentration. A foal blood sample was collected 18 h after birth to determine passive transfer of IgG. Placental, mare, and foal weights were measured at birth. Data were analyzed using a t-test with significance at P ≤ 0.05 and a tendency at 0.05 &lt; P ≤ 0.10. The MO group had greater BCS (P = 0.01) and CNS (P = 0.01) than the LN group. Moderately obese mares tended to have 9% greater glucose area under the curve (AUC) after dextrose (P = 0.10) and a 9% greater glucose AUC after insulin infusion (P = 0.10) than LN mares. Colostrum IgG concentrations, and protein and fat content (P ≥ 0.3) did not differ between groups. Foal serum IgG concentrations, foal BW, and placental weights were similar between groups (P ≥ 0.8). The ratios between foal BW and placenta weight, and between foal BW and mare BW were similar between groups (P ≥ 0.3). Moderately obese maternal BCS and CNS scores may decrease maternal glucose clearance during late gestation but did not impact colostrum quality or foal IgG concentrations within 24 h postpartum. As the equine fetus matures later in gestation than other livestock species and relies heavily on glucose uptake from the placenta, it is critical to increase our understanding of the impacts of maternal obesity and glucose clearance on foal development and pre- and post-natal health.
228 Evaluation of sire vs. dam influences on age at first parturition in Brahman heifers
Authors: Willis Caleta , Earnhardt-San Audrey , Carson Chloey P , Long Charles R , Welsh Thomas H , Randel Ronald D , Perry George A 
Published: September 2024

Abstract
The objective of this study was to determine if sire or dam had a stronger impact on age at first parturition of Brahman heifers (n = 833) born between 2000-2021. Typically, in Bos taurus heifers, puberty occurs between 10 to 12 mo of age leading to parturition of the first calf occurring around 24 mo of age. However, in Bos indicus cattle, puberty is achieved between 15 to 17 mo of age leading to a first calf around 36 mo of age. The ability of heifers to achieve pubertal status earlier plays a vital role in reproductive success. For selection purposes, knowing if early pubertal attainment is influenced more by the sire or dam is important for herd management. For this study, to minimize any epigenetic and environmental changes, all heifers originated and were developed at the Texas A&M AgriLife Research Center at Overton. Sire groups (SG) and dam groups (DG) were established based on the number of offspring used in the study. Sire groups were comprised of SG-1 (1+ calves sired; n = 58 sires), SG-2 (5+ calves sired; n = 44 sires), SG-3 (10+ calves sired; n = 31 sires), SG-4 (15+ calves sired; n = 23 sires), SG-5 (20+ calves sired; n = 17 sires), and SG-6 (25+ calves sired; n = 12 sires). Dam groups consisted of DG-1 (1+ calves; n = 489 dams), DG-2 (2+ calves; n = 215 dams), DG-3 (3+ calves; n = 81 dams), DG-4 (4+ calves; n = 38 dams), and DG-5 (5+ calves; n = 9 dams). Statistical analysis (PROC GLM; SAS 9.4) included the fixed effect of sire group (dam group) and all dams (sires) utilized in that grouping. Significance for age at first calving was considered at P &lt; 0.05. Mean separation for age was performed using LSmeans. Sire significantly impacted age at first calving in SG-1 (P = 0.0173) vs dam effect (P = 0.3993; n = 489 dams), SG-2 (P = 0.0159) vs dam effect (P = 0.5476; n = 481 dams), SG-3 (P = 0.0023) vs dam effect (P = 0.2532; n = 439 dams), SG-4 (P = 0.0022) vs dam effect (P = 0.3997; n = 400 dams), SG-5 (P = 0.0002) vs dam effect (P = 0.0602; n = 361 dams), and SG-6 (P = 0.0005) vs dam effect (P = 0.1058; n = 305 dams). The dam impact on age at first calving was never significant in the dam groupings but sires did impact age at first calving in two groups: DG-1 (P = 0.3993) vs sire effect (P = 0.0173; n = 58 sires), DG-2 (P = 0.4823) vs sire effect (P = 0.0173; n = 56 sires), DG-3 (P = 0.3011) vs sire effect (P = 0.3420; n = 49 sires), DG-4 (P = 0.3911) vs sire effect (P = 0.6278; n = 40 sires), and DG-5 (P = 0.3042) vs sire effect (P = 0.9159; n = 23 sires). In summary, evaluation of sire and dam influences on age at first parturition for female offspring demonstrates that the ability to calve at an earlier age is influenced by the sire but not the dam. Further studies into the mechanisms for the transmission of this trait are warranted.
226 Serotonin stimulates relaxation in ovine saphenous vein precontracted with ergovaline
Authors: Klotz James L , Duckett Susan K , Checura Celina M 
Published: September 2024

Abstract
The predominant ergot alkaloid found in wild type endophyte-infected tall fescue grass is ergovaline. Ergovaline is a potent vasoconstrictor in livestock that consume it. Previous research has shown that ergovaline can bind serotonin (5-HT) receptors and blood vessels exposed to ergovaline become unresponsive to subsequent 5-HT exposure. Serotonin can stimulate vascular contraction or relaxation depending on the receptor subtype targeted. The objective of this experiment was to evaluate 5HT as vasorelaxant in the ovine saphenous artery and vein that were precontracted with ergovaline. Blood vessels were collected from mixed breed market rams (n = 7) at slaughter, cleaned of surrounding adipose and connective tissue, sliced into 2-mm cross-sections, and mounted in a multimyograph. Artery and vein cross-sections were equilibrated to 1 g tension in continuously gassed (95% O2/5% CO2) Krebs-Henseleit buffer (pH 7.4; 37.5 °C) for 90 min, precontracted with 1.0 μM ergovaline (in a tall fescue seed extract) for 30 min, exposed to increasing concentrations of 5-HT that ranged from 1 x 10-9 to 1 x 10-4 M for a 5-min interval. Response data were normalized to the 1.0 μM ergovaline response and were analyzed as a completely randomized design in SAS. The saphenous vein was larger in diameter blood vessel than the saphenous artery. Both vessel types contracted in response to exposure to 1 μM ergovaline. The saphenous artery had a much lower maximal response to ergovaline than did the vein (P &lt; 0.05) that could be attributed to the size difference. The lateral saphenous vein relaxed to increasing concentrations of 5-HT (P &lt; 0.05) with 87 % relaxation occurring by 1 x 10-6 M 5-HT. Conversely, the lateral saphenous artery contracted in response to increasing concentrations of 5-HT (P &lt; 0.05) resulting in a 43% increase from the ergovaline response by the 1 x 10-4 M addition. This response did not become significant in the artery until 1 x 10-5 M 5-HT compared with 1 x 10-8 M for the vein. This resulted in a 50% effective concentration for 5-HT in the artery of 4.5 compared with 8.1 M for the vein (P &lt; 0.05). The differing responses across vessels are likely a result of different receptor populations and this should be verified in future research. This is the first reported observation of stimulated relaxation of a blood vessel constricted ergovaline. Additional research is needed to understand the mechanism of how 5HT can be used to stimulate vasorelaxation in livestock suffering from ergot alkaloid-induced vasoconstriction.