bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monocyte Induction of E-Selectin–Mediated Endothelial Activation Releases VE-Cadherin Junctions to Promote Tumor Cell Extravasation in the Metastasis Cascade
Authors: Häuselmann Irina , Roblek Marko , Protsyuk Darya , Huck Volker , Knopfova Lucia , Grässle Sandra , Bauer Alexander T. , Schneider Stefan W. , Borsig Lubor 
Published: August 2016

Abstract
Tumor cells interact with blood constituents and these interactions promote metastasis. Selectins are vascular receptors facilitating interactions of tumor cells with platelets, leukocytes, and endothelium, but the role of endothelial E-selectin remains unclear. Here we show that E-selectin is a major receptor for monocyte recruitment to tumor cell–activated endothelium. Experimental and spontaneous lung metastasis using murine tumor cells, without E-selectin ligands, were attenuated in E-selectin–deficient mice. Tumor cell–derived CCL2 promoted endothelial activation, resulting in enhanced endothelial E-selectin expression. The recruitment of inflammatory monocytes to metastasizing tumor cells was dependent on the local endothelial activation and the presence of E-selectin. Monocytes promoted transendothelial migration of tumor cells through the induction of E-selectin–dependent endothelial retractions and a subsequent modulation of tight junctions through dephosphorylation of VE-cadherin. Thus, endothelial E-selectin shapes the tumor microenvironment through the recruitment, adhesion, and activation of monocytes that facilitate tumor cell extravasation and thereby metastasis. These findings provide evidence that endothelial E-selectin is a novel factor contributing to endothelial retraction required for efficient lung metastasis. Cancer Res; 76(18); 5302–12. ©2016 AACR.
ADAM17 is a Tumor Promoter and Therapeutic Target in Western Diet–associated Colon Cancer
Authors: Mustafi Reba , Dougherty Urszula , Mustafi Devkumar , Ayaloglu-Butun Fatma , Fletcher Michelle , Adhikari Sarbani , Sadiq Farhana , Meckel Katherine , Haider Haider I. , Khalil Abdurahman , Pekow Joel , Konda Vani , Joseph Loren , Hart John , Fichera Alessandro , Li Yan Chun , Bissonnette Marc 
Published: August 2016

Abstract
Purpose: Epidermal growth factor receptors (EGFR) are required for tumor promotion by Western diet. The metalloprotease, ADAM17 activates EGFR by releasing pro-EGFR ligands. ADAM17 is regulated by G-protein–coupled receptors, including CXCR4. Here we investigated CXCR4–ADAM17 crosstalk and examined the role of ADAM17 in tumorigenesis.
Experimental Design: We used CXCR4 inhibitor, AMD3100 and ADAM17 inhibitor, BMS566394 to assess CXCR4–ADAM17 crosstalk in colon cancer cells. We compared the expression of CXCR4 ligand, CXCL2, and ADAM17 in mice fed Western diet versus standard diet. Separately, mice were treated with marimastat, a broad-spectrum ADAM17 inhibitor, or AMD3100 to assess EGFR activation by ADAM17 and CXCR4. Using Apc-mutant Min mice, we investigated the effects of ADAM17/10 inhibitor INCB3619 on tumorigenesis. To assess the effects of colonocyte ADAM17, mice with ADAM17 conditional deletion were treated with azoxymethane (AOM). ADAM17 expression was also compared in colonocytes from primary human colon cancers and adjacent mucosa.
Results: CXCL12 treatment activated colon cancer cell EGFR signals, and CXCR4 or ADAM17 blockade reduced this activation. In vivo, Western diet increased CXCL12 in stromal cells and TGFα in colonocytes. Marimastat or AMD3100 caused &gt;50% reduction in EGFR signals (P &lt; 0.05). In Min mice, INCB3619 reduced EGFR signals in adenomas and inhibited intestinal tumor multiplicity (P &lt; 0.05). In the AOM model, colonocyte ADAM17 deletion reduced EGFR signals and colonic tumor development (P &lt; 0.05). Finally, ADAM17 was upregulated &gt;2.5-fold in human malignant colonocytes.
Conclusions: ADAM17 is a Western diet–inducible enzyme activated by CXCL12–CXCR4 signaling, suggesting the pathway: Western diet→CXCL12→CXCR4→ADAM17→TGFα→EGFR. ADAM17 might serve as a druggable target in chemoprevention strategies. Clin Cancer Res; 23(2); 549–61. ©2016 AACR.
Glasgow Gloom or Leeds Glue? Dialect-Specific Vowel Duration Constrains Lexical Segmentation and Access
Authors: Smith Rachel , Rathcke Tamara 
Published: August 2016

Abstract
Timing cues are important in many aspects of speech processing, fromidentifying segments to locating word and phrase boundaries. They vary across accents, yet representation and processing of this variation are poorly understood. We investigated whether an accent difference in vowel duration affects lexical segmentation and access. In Glasgow English (GE), /i u e o/ are shorter than in Leeds English (LE), especially for /i u/ before voiced stops and nasals. In a word-spotting experiment, GE and LE participants heard nonsense sequences (e.g. pobegloomezh) containing embedded words (gloom, glue), with segmental qualities intermediate between GE and LE. Critical vowel durations were manipulated according to accent (GE-appropriate vowels shorter than LE-appropriate ones) and phonological context (vowels shortest before voiceless stops < voiced stops/nasals < voiced fricatives). GE participants generally spotted words like gloom more accurately with GE-appropriate than LE-appropriate vowels. LE participants were less accurate than GE participants to spot words like gloom with GE-appropriate vowels, but more likely to spot embeddings like glue. These results were broadly as predicted based on the accent differences, but depended less than expected on the accent-specific phonological constraints. We discuss theoretical implications regarding the representation of duration and the time course of lexical access.
A preliminary checklist of the skippers (Rhopalocera: Hesperiidae) of Guangxi, China
Authors: XUE GUO-XI , SUN HAO , SUN ZHOU , WANG SHAO-NENG 
Published: August 2016

A survey of the literature shows that a total of 64 genera comprising 155 species/subspecies of skippers have been recorded in Guangxi. Based on a field survey and the examination of specimens, 1 subspecies, Burara gomata gomata (Moore, 1866), is recorded as new to China, and 1 species, Celaenorrhinus vietnamicus Devyatkin, 1998, is reported in Guangxi for the first time. Therefore, to date, a total of 57 genera with 109 species/subspecies in 4 subfamilies of Hesperiidae have been positively identified as the fauna of Guangxi. Four genera and 42 species/subspecies are considered as requiring confirmation, and 3 genera and 6 species/subspecies are excluded. Some taxonomic notes are provided when necessary.
The Seasonal Cycle of the Radiation Budget and Cloud Radiative Effect in the Amazon Rain Forest of Brazil
Authors: Marquardt Collow Allison B. , Miller Mark A. 
Published: August 2016

Abstract
Changes in the climate system of the Amazon rain forest of Brazil can impact factors that influence the radiation budget such as clouds, atmospheric moisture, and the surface albedo. This study examines the relationships between clouds and radiation in this region using surface observations from the first year of the deployment of the Atmospheric Radiation Measurement (ARM) Program’s Mobile Facility 1 (AMF1) in Manacapuru, Brazil, and satellite measurements from the Clouds and the Earth’s Radiant Energy System (CERES). The seasonal cycles of the radiation budget and cloud radiative effects (CREs) are evaluated at the top of the atmosphere (TOA), at the surface, and within the atmospheric column using these observations and are placed into a regional context using the Modern-Era Retrospective Analysis for Research and Applications, version 2 (MERRA-2). Water vapor and clouds are abundant throughout the year, even though slight decreases are observed in the dry season. The column water vapor load is large enough that the longwave radiative flux divergence is nearly constant throughout the year. Clouds produce a significant shortwave CRE at the surface and TOA, exceeding 200 W m−2 during the wet season. Discrepancies, especially in column shortwave radiative absorption, between the observations and MERRA-2 are demonstrated that warrant additional analysis of the microphysical and macrophysical cloud properties in MERRA-2. More trustworthy fields in the MERRA-2 product suggest that the expansive nearby river system impacts the regional radiation budget and thereby renders AMF1 observations potentially biased relative to regions farther removed from rivers within the Amazon rain forest.
Water Masses and Circulation in the Tropical Pacific off Central Mexico and Surrounding Areas
Authors: Portela Esther , Beier Emilio , Barton Eric D. , Castro Rubén , Godínez Victor , Palacios-Hernández Emilio , Fiedler Paul C. , Sánchez-Velasco Laura , Trasviña Armando 
Published: August 2016

AbstractThe seasonal variations and the interactions of the water masses in the tropical Pacific off central Mexico (TPCM) and four surrounding areas were examined based on an extensive new hydrographic database. The regional water masses were redefined in terms of absolute salinity (SA) and conservative temperature (Θ) according to the Thermodynamic Equation of Seawater 2010 (TEOS-10). Hydrographic data and the evaporation minus (precipitation + runoff) balance were used to investigate the origin and seasonality of two salinity minima in the area. The shallow (50–100 m) salinity minimum originates with the California Current System and becomes saltier as it extends southeastward and mixes with tropical subsurface waters while the surface salinity minimum extends farther north in the TPCM in summer and fall because of the northward advection of tropical surface waters. The interactions between water masses allow a characterization of the seasonal pattern of circulation of the Mexican Coastal Current (MCC), the tropical branch of the California Current, and the flows through the entrance of the Gulf of California. The seasonality of the MCC inferred from the distribution of the water masses largely coincides with the geostrophic circulation forced by an annual Rossby wave.
Assessment of Administrative Medical Claims Data for Public Health Surveillance of Invasive Group A Streptococcal Necrotizing Fasciitis in Tennessee
Authors: Lindegren Mary Lou , McCormack Leigh , Barnes Brenda , Mitchel Ed , Jones Stephen , Schaffner William 
Published: August 2016

Objective. Necrotizing fasciitis (NF) is a severe manifestation of invasive group A streptococcal (iGAS) infection. NF is a rapidly progressive infection of the subcutaneous tissues, including the fascia, and accurate diagnosis and prompt treatment are critical. Population-based surveillance is conducted for iGAS, including the severe manifestations of NF, by the federally funded Active Bacterial Core surveillance (ABCs) program. Methods. We used administrative claims data from a large managed care organization in Tennessee to enhance the public health surveillance for NF iGAS. For the period 2003–2012, we identified cases of NF in Tennessee by searching the claims database for diagnostic codes indicating the diagnosis of NF. We compared these cases with cases detected in selected Tennessee counties by ABCs. Results. Of 356 managed care patients with a diagnostic code indicating NF, we determined that 20 (6%) patients had been hospitalized with iGAS infection and, therefore, were likely to be true NF cases. Of these 20 patients, only 11 matched with patients identified by ABCs; nine patients had not been previously identified by ABCs. During the same time period, 54 patients with NF were ascertained by ABCs. Conclusion. Administrative claims data have the potential to augment disease surveillance but require a large investment in resources compared with the few NF cases identified.
Effects of Health Level 7 Messaging on Data Quality in New York City’s Immunization Information System, 2014
Authors: Metroka Amy E. , Papadouka Vikki , Ternier Alexandra , Zucker Jane R. 
Published: August 2016

Objective. We compared the quality of data reported to New York City’s immunization information system, the Citywide Immunization Registry (CIR), through its real-time Health Level 7 (HL7) Web service from electronic health records (EHRs), with data submitted through other methods. Methods. We stratified immunizations administered and reported to the CIR in 2014 for patients aged 0–18 years by reporting method: ( 1) sending HL7 messages from EHRs through the Web service, ( 2) manual data entry, and ( 3) upload of a non-standard flat file from EHRs. We assessed completeness of reporting by measuring the percentage of immunizations reported with lot number, manufacturer, and Vaccines for Children (VFC) program eligibility. We assessed timeliness of reporting by determining the number of days from date of administration to date entered into the CIR. Results. HL7 reporting accounted for the largest percentage (46.3%) of the 3.8 million immunizations reported in 2014. Of immunizations reported using HL7, 97.9% included the lot number and 92.6% included the manufacturer, compared with 50.4% and 48.0% for manual entry, and 65.9% and 48.8% for non-standard flat file, respectively. VFC eligibility was 96.9% complete when reported by manual data entry, 95.3% complete for HL7 reporting, and 87.2% complete for non-standard flat file reporting. Of the three reporting methods, HL7 was the most timely: 77.6% of immunizations were reported by HL7 in <1 day, compared with 53.6% of immunizations reported through manual data entry and 18.1% of immunizations reported through non-standard flat file. Conclusion. HL7 reporting from EHRs resulted in more complete and timely data in the CIR compared with other reporting methods. Providing resources to facilitate HL7 reporting from EHRs to immunization information systems to increase data quality should be a priority for public health.
Diphenhydramine as a selective probe to study H+-antiporter function at the blood–brain barrier: Application to [11C]diphenhydramine positron emission tomography imaging
Authors: Auvity Sylvain , Chapy Hélène , Goutal Sébastien , Caillé Fabien , Hosten Benoit , Smirnova Maria , Declèves Xavier , Tournier Nicolas , Cisternino Salvatore 
Published: August 2016

Diphenhydramine, a sedative histamine H1-receptor (H1R) antagonist, was evaluated as a probe to measure drug/H+-antiporter function at the blood–brain barrier. In situ brain perfusion experiments in mice and rats showed that diphenhydramine transport at the blood–brain barrier was saturable, following Michaelis–Menten kinetics with a Km = 2.99 mM and Vmax = 179.5 nmol s−1 g−1. In the pharmacological plasma concentration range the carrier-mediated component accounted for 77% of diphenhydramine influx while passive diffusion accounted for only 23%. [14C]Diphenhydramine blood–brain barrier transport was proton and clonidine sensitive but was influenced by neither tetraethylammonium, a MATE1 (SLC47A1), and OCT/OCTN (SLC22A1-5) modulator, nor P-gp/Bcrp (ABCB1a/1b/ABCG2) deficiency. Brain and plasma kinetics of [11C]diphenhydramine were measured by positron emission tomography imaging in rats. [11C]Diphenhydramine kinetics in different brain regions were not influenced by displacement with 1 mg kg−1 unlabeled diphenhydramine, indicating the specificity of the brain positron emission tomography signal for blood–brain barrier transport activity over binding to any central nervous system target in vivo. [11C]Diphenhydramine radiometabolites were not detected in the brain 15 min after injection, allowing for the reliable calculation of [11C]diphenhydramine brain uptake clearance (Clup = 0.99 ± 0.18 mL min−1 cm−3). Diphenhydramine is a selective and specific H+-antiporter substrate. [11C]Diphenhydramine positron emission tomography imaging offers a reliable and noninvasive method to evaluate H+-antiporter function at the blood–brain barrier.
Angiotensin II Type 1 receptor (AGTR1) gene polymorphisms are associated with vascular manifestations in patients with systemic sclerosis (SSc)
Authors: Rodríguez-Reyna Tatiana S , Núñez-Alvarez Carlos , Cruz-Lagunas Alfredo , Posadas-Sánchez Rosalinda , Pérez-Hernández Nonanzit , Jiménez-Alvarez Luis , Ramírez-Martínez Gustavo , Granados Julio , Vargas-Alarcón Gilberto , Zúñiga Joaquín 
Published: August 2016

Introduction: Systemic sclerosis (SSc) shows variable clinical expression in different ethnic groups; vascular abnormalities are a prominent feature of this disease and its clinical expression may be influenced by genetic factors. Patients and methods: Herein, we describe 15 polymorphisms of the renin-angiotensin-aldosterone pathway in 170 Mexican admixed SSc patients (defined as patients with Mexican ancestry for at least 3 generations) and 199 healthy controls. We determined the presence of angiotensin II Type 1 receptor (AGTR1), angiotensin converting enzyme (ACE) and Endothelin 1 single nucleotide polymorphisms (SNPs) using 5’ exonuclease TaqMan genotyping assays on a 7900HT real-time fast polymerase chain reaction (PCR) system. Results: These polymorphisms had a similar distribution between SSc patients and controls, but we found that the AGTR1 G-680T (rs275652) ( p = 0.02; OR 3.5; 95%CI 1.2–10.4) and AGTR1 A-119G (rs275653) ( p = 0.008; OR 4.2; 95% CI 1.5–12.1) polymorphisms were associated with severe vascular involvement in our SSc patients. Conclusions: This is the first report of the association of these polymorphisms with vasculopathy in Mexican admixed SSc patients. Our findings suggested that the angiotensin II Type 1 receptor genotype may influence the clinical expression of vasculopathy in these patients. Functional analyses should follow.
Recruitment methods in a clinical trial of provoked vulvodynia: Predictors of enrollment
Authors: Bachour Candi C , Bachmann Gloria A , Foster David C , Wan Jim Y , Rawlinson Leslie A , Brown Candace S ,   Gabapentin Study Group (GABAgroup)
Published: August 2016

Background: Successful recruitment in clinical trials for chronic pain conditions is challenging, especially in women with provoked vulvodynia due to reluctance in discussing pain associated with sexual intercourse. The most successful recruitment methods and the characteristics of women reached with these methods are unknown. Objective: To compare the effectiveness and efficiency of four recruitment methods and to determine socioeconomic predictors for successful enrollment in a National Institutes of Health–sponsored multicenter clinical trial evaluating a gabapentin intervention in women with provoked vulvodynia. Methods: Recruitment methods utilized mass mailing, media, clinician referrals and community outreach. Effectiveness (number of participants enrolled) and efficiency (proportion screened who enrolled) were determined. Socioeconomic variables including race, educational level, annual household income, relationship status, age, menopausal status and employment status were also evaluated regarding which recruitment strategies were best at targeting specific cohorts. Results: Of 868 potential study participants, 219 were enrolled. The most effective recruitment method in enrolling participants was mass mailing ( p < 0.001). There were no statistically significant differences in efficiency between recruitment methods ( p = 0.11). Relative to clinician referral, black women were 13 times as likely to be enrolled through mass mailing (adjusted odds ratio 12.5, 95% confidence interval, 3.6–43.1) as white women. There were no differences in enrollment according to educational level, annual income, relationship status, age, menopausal status, or employment status and recruitment method. Conclusion: In this clinical trial, mass mailing was the most effective recruitment method. Race of participants enrolled in a provoked vulvodynia trial was related to the recruitment method.
Human Articular Chondrocytes Induce Interleukin-2 Nonresponsiveness to Allogeneic Lymphocytes
Authors: Abe Satomi , Nochi Hitoshi , Ito Hiroshi 
Published: August 2016

IntroductionWe previously showed that articular chondrocytes (ACs) have immune privilege and immunomodulatory functions like those of mesenchymal stem cells. To elucidate these mechanisms, we focused on interleukin-2 (IL-2), which plays critical roles in lymphocyte mitogenic activity. The purpose of this study was to explore whether ACs affect the role of IL-2 underlying immunomodulatory functions.Material and MethodsIrradiated human ACs from osteoarthritis donors were used. Third-party ACs were added to the mixed lymphocyte reaction (MLR) with or without recombinant human IL-2 (rhIL-2), and the levels of IL-2 and the soluble form of the IL-2 receptor α (sIL-2Rα) protein in supernatant were measured by enzyme-linked immunosorbent assay. Recombinant human IL-2 (rhIL-2) was also added to the MLR. To detect the expression of IL-2 receptor α (CD25) on lymphocytes in the MLR, flow cytometric analysis was performed. Last, ACs and allogeneic activated CD4+T cell were co-cultured, and the expression of CD25 on activated T cells was examined by flow cytometry.ResultsThird-party ACs significantly inhibited the MLR and reduced the level of sIL-2Rα in a dose-dependent manner, but did not affect the concentration of IL-2. Exogenous rhIL-2 accelerated MLR but did not rescue the inhibitory effect of ACs. ACs inhibited the expression of CD25 on activated CD4+T cells.DiscussionOur results showed that third-party ACs inhibited the proliferation of allogeneic activated lymphocytes, thereby inhibiting production sIL-2Rα, although ACs did not affect IL-2 secretion from lymphocytes. Also, ACs inhibited CD25 expression on activated CD4+T cells. Thus, ACs inhibited the immune response of allogeneic lymphocytes by inducing IL-2 nonresponsiveness.
Perspectives of caregivers towards physiotherapy treatment for children with burns in Harare, Zimbabwe: A cross-sectional study
Authors: Chiwaridzo Matthew , Zinyando Vimbayinashe Juliet , Dambi Jermaine Matewu , Kaseke Farayi , Munambah Nyaradzai , Mudawarima Tapfuma 
Published: August 2016

Abstract

Background
Physiotherapy is an integral part of treatment for paediatric burns. In Zimbabwe, children are admitted in paediatric burn unit with their caregivers, who play important roles such as providing explanation and obtaining cooperation of the child during physiotherapy, which is often uncomfortable or painful to the patient. The aim of this study was to determine the perspectives of caregivers towards physiotherapy interventions administered to hospitalized children at central hospitals in Harare, Zimbabwe.

Methods
A descriptive cross-sectional study was conducted using self-administered questionnaires. The study was carried out at two large central hospitals (Parirenyatwa Hospital and Harare Central Hospital). The study targeted all the caregivers of children below the age of 12 years with a diagnosis of burns, irrespective of severity or area affected, who were admitted in the two paediatric burn units. Of the 34 caregivers eligible to participate, 31 (91.1 %) questionnaires had complete data and were analysed. The analyses were done using Statistica version 12.0.

Results
The median age of the caregivers was 28 years (IQR = 24–33 years). Female caregivers constituted 90.3 % of the sample. The majority of the caregivers (n = 26, 83.9 %) were biological mothers to the hospitalised child. The majority of children (n = 20, 64.5 %) hospitalised were between 0 and 4 years. The commonest cause of burns was scalding (n = 19, 61.2 %). The burns were mainly in the upper extremities (n = 11, 35.5 %). Physiotherapy for the burns was mainly active and passive joint range of motion exercises (n = 30, 96.8 %). The caregivers’ perceptions towards physiotherapy were mainly positive (n = 20, 64.5 %) indicating that physiotherapy plays an important role in burn management. Of the 21 caregivers given a ward exercise programme, 13 (61.9 %) were not compliant.

Conclusions
Caregivers’ perspectives towards physiotherapy were largely positive and are similar to those found in other studies. The majority of the caregivers indicated that physiotherapy was important in the overall management of burns leading to proper healing of the wounds without complications.