bluuebunny/crossref_metadata_2025_split huggingface dataset:
Choice of Frontline Tyrosine‐Kinase Inhibitor and Early Events in Very Elderly Patients With Chronic Myeloid Leukemia in Chronic Phase: A “Campus CML” Study
Authors: Bucelli C. , Capodanno I. , Miggiano M. C. , Cavazzini F. , Crescenzi S. Leonetti , Russo S. , Carmosino I. , Annunziata M. , Sorà F. , Bonifacio M. , Luciano L. , Caocci G. , Loglisci G. , Elena C. , Lunghi F. , Mullai R. , Attolico I. , Binotto G. , Crisà E. , Sportoletti P. , Di Veroli A. , Scortechini A. R. , Leporace A. P. , Maggi A. , Crugnola M. , Stagno F. , Sancetta R. , Murgano P. , Rapezzi D. , Luzi D. , Vincelli D. I. , Galimberti S. , Bocchia M. , Fava C. , Malato A. , Abruzzese E. , Saglio G. , Specchia G. , Breccia M. , Iurlo A. , Tiribelli M. , Latagliata R. 
Published: September 2024

ABSTRACTObjectivesThe study aimed to evaluate the utilization of frontline TKI therapy in a large cohort of elderly CP‐CML patients.MethodsA retrospective analysis was conducted on 332 CP‐CML patients aged 75 years or older among 1929 diagnosed from January 2012 to December 2019 followed at 36 participating Hematology Centers involved in the “Campus CML” project.ResultsAmong the patients analyzed, 85.8% received imatinib (IM) while 14.2% received second‐generation TKIs (2G‐TKI), 59.5% dasatinib, and 40.5% nilotinib. Most patients initiated IM at standard dose (67.3%) while 32.7% at reduced dose. A similar trend was observed with 2G‐TKIs. The cumulative incidence of permanent TKI discontinuation at 12 months was 28.4%, primarily due to primary resistance (10.1%) and extra‐hematologic toxicity (9.5%), with no significant difference between IM and 2G‐TKI groups. Following the introduction of generic IM in Italy in 2018, IM usage increased significantly compared with 2G‐TKIs.ConclusionsIM was in our Centers the preferred frontline therapy for older CP‐CML patients, with increasing utilization after the introduction of generic formulations. However, 2G‐TKIs are still used in a substantial proportion of patients, suggesting individualized physician assessments regarding patient suitability and expectations. Further investigation is needed to assess efficacy and safety of reduced TKI doses in this patient population.
Dual trajectories of supportive coparenting and father involvement: Parental predictors and influences
Authors: Park SuJung , Barnett Melissa A. , Mastergeorge Ann M. 
Published: September 2024

AbstractObjectiveThe goal of this study was to examine dual trajectories of supportive coparenting and father involvement across the transition to parenthood into early childhood.BackgroundDuring the transition to parenthood, families adapt to new dynamic family subsystems. Drawing on family systems theory, we investigated the influence of supportive coparenting (mother–father subsystem) and father involvement (father–child subsystem) on child development.MethodLongitudinal data from the Future of Families and Child Wellbeing Study collected at the child's birth and ages 1, 3, and 5 years included a sample of predominantly unmarried disadvantaged couples (N = 856) living in diverse couple relationship configurations. Group‐based modeling was used to identify interfamilial variations in dual trajectories and how these trajectory groups were associated with parental predictors and child behavior outcomes.ResultsThree classes were identified: High‐Slow Decreasing Supportive Coparenting and Father Involvement (Class 1, 78%), High‐Rapid Decreasing Supportive Coparenting and Father Involvement (Class 2, 12%), and Low‐Increasing Supportive Coparenting and Low‐Stable Father Involvement (Class 3, 10%). Class 3 showed greater couple supportiveness than the other classes. Couples in committed partner relationships were more likely to be in Class 1 or Class 2 than Class 3. Children of Class 1 displayed fewer behavioral problems than the other classes.ConclusionsFindings emphasize the value of jointly considering coparenting and father involvement in targeted interventions to strengthen child development and family functioning among economically disadvantaged families.
Attitudes and Behaviours Regarding COVID‐19 Mitigation Strategies in Australians With an Underlying Health Condition: A Cross‐Sectional Study
Authors: Soh Sze‐Ee , Ayton Darshini , Bevins Amelia , Skouteris Helen , Trent Mallory , MacIntyre Raina 
Published: September 2024

ABSTRACTBackgroundPublic health strategies have focused on preventing and slowing the transmission of COVID‐19 by promoting the uptake of mitigation strategies. However, little is known about the uptake of these strategies in the presence of underlying health conditions.ObjectivesTo describe the attitudes and behaviours of a sample of Australians towards COVID‐19 mitigation strategies, and determine if uptake of these strategies differed across different health conditions.DesignCross‐sectional study.Setting and ParticipantsNational survey of Australian residents over 18 years.Main Outcome MeasuresA purpose‐built survey was used to collect participants' attitudes and behaviours towards COVID‐19 mitigation strategies.ResultsOver half (53%) of the 2867 participants (99% completion rate) reported having one or more comorbidities. The most commonly self‐reported health condition was cardiometabolic conditions (28%). Most participants disagreed that masks were no longer needed (74%) and wanted the 5‐day isolation mandate (66%). More than one‐third would like masks to be mandated for indoor spaces (38%) and 25% avoided going to hospitals. Participants with allergies (OR 1.37; 95% CI 1.14, 1.65), cardiometabolic (OR 1.49; 95% CI 1.23, 1.79), respiratory (OR 1.32; 95% CI 1.07, 1.62) and neurological (OR 1.62; 95% CI 1.12, 2.32) conditions were more likely to avoid using public transport compared to those without. In contrast, participants with underlying mental health conditions were less likely to use N95/P2 facemasks in public spaces (OR 0.46; 95% CI 0.25, 0.87) compared to those without.ConclusionsA substantial proportion of Australians continued to adopt COVID‐19 mitigation measures or expressed a desire for more mitigations, including mandatory isolation for COVID‐19, despite the lack of mandates. People with an underlying health condition who represent more than half of all adults appear to be more careful with mitigations to avoid COVID‐19.Patient or Public ContributionMembers of the public were invited to participate in a soft launch of the survey between 4th and 5th January 2023 to test flow and functionality, and to allow the final wording of survey questions to be refined as required.
XP‐Endo Finisher behaviour and irrigant temperature in intracanal environment: Clinical measurements
Authors: Silva Fernanda de Melo , Macedo Isabella Figueiredo Assis , Resende Pedro Damas , Tavares Warley Luciano Fonseca , Buono Vicente Tadeu Lopes , Santos Leandro de Arruda , Peixoto Isabella Faria da Cunha , Viana Ana Cecília Diniz 
Published: September 2024

AbstractAimTo evaluate the behaviour of the XP‐Endo Finisher and the variation in the intracanal temperature of the irrigant at rest and when activated over time.MethodologyDifferential scanning calorimetry (DSC) determined the transformation temperatures of XP‐Endo Finisher instruments. A digital thermocouple was used to measure the temperature of the irrigant inside the pulp chamber. Two measurements were performed for each tooth (n = 12): with the irrigant at rest and during XP‐Endo agitation for 60 s to observe the temperature evolution. The data were statistically analysed using a t‐test with a confidence level of 95%.ResultsDSC results suggested that the XP‐Endo Finisher had a mixed R‐phase and austenitic structure at room temperature. The temperature values at predetermined time points (0, 10, 40, 70, 120 and 240 s) were measured, and no statistical difference was observed between the values of the resting and activated solutions at any of the selected points (p > .05). For the protocol performed with the XP‐Endo file, the mean irrigant temperature observed at instrument insertion inside the root canal was 28.65°C. After 60 s of agitation, the temperature was 34.02°C. The solution temperature stabilized inside the canal only after 211 s at 35.5°C. The mean maximum irrigation solution temperature recorded inside the canal was 35.5°C without agitation and after XP‐endo agitation (p > .05).ConclusionsAlthough the XP‐Endo Finisher system does not promote heating of the irrigation solution, file expansion responsible for improved instrument cleaning starts at a temperature below the expected value.
Sustainable valorisation of freshwater fish by‐products to gelatin and fish oil by hydrothermal extraction
Authors: Adnan Saepul , Kresnowati Made Tri Ari Penia , Marlina  , Bindar Yazid 
Published: September 2024

SummaryThis study explores the potential of hydrothermal extraction as an innovative valorisation method for the simultaneous production of gelatin and fish oil from fish byproducts. It offers an environmentally friendly alternative to conventional solvent‐based techniques by minimising the use of chemical solvents and water. We researched various major commercial freshwater fish species: striped catfish, catfish, and tilapia, and achieved a gelatin product that reflects commercial standards in chemical structure, gel strength, and pH level. The striped catfish gelatin showed the highest gel strength and viscosity (66.01 ± 0.83 g bloom and 15.3 ± 0.1 cP), but was produced at a lower yield than the catfish gelatin. On the other hand, striped catfish produced the highest yield of fish oil (13.56 ± 0.21%) and has the highest omega 3 content (3.72 ± 0.02%) than other fish oil. This innovative study demonstrates the potential of hydrothermal extraction as a sustainable method for producing gelatin and fish oil, offering eco‐friendly and nutritional benefits across a range of applications.
Inherent metabolic preferences differentially regulate the sensitivity of Th1 and Th2 cells to ribosome‐inhibiting antibiotics
Authors: Jawla Neha , Kar Raunak , Patil Veena S. , Arimbasseri G. Aneeshkumar 
Published: September 2024

AbstractMitochondrial translation is essential to maintain mitochondrial function and energy production. Mutations in genes associated with mitochondrial translation cause several developmental disorders, and immune dysfunction is observed in many such patients. Besides genetic mutations, several antibiotics targeting bacterial ribosomes are well‐established to inhibit mitochondrial translation. However, the effect of such antibiotics on different immune cells is not fully understood. Here, we addressed the differential effect of mitochondrial translation inhibition on different subsets of helper T cells (Th) of mice and humans. Inhibition of mitochondrial translation reduced the levels of mitochondrially encoded electron transport chain subunits without affecting their nuclear‐encoded counterparts. As a result, mitochondrial oxygen consumption reduced dramatically, but mitochondrial mass was unaffected. Most importantly, we show that inhibition of mitochondrial translation induced apoptosis, specifically in Th2 cells. This increase in apoptosis was associated with higher expression of Bim and Puma, two activators of the intrinsic pathway of apoptosis. We propose that this difference in the sensitivity of Th1 and Th2 cells to mitochondrial translation inhibition reflects the intrinsic metabolic demands of these subtypes. Though Th1 and Th2 cells exhibit similar levels of oxidative phosphorylation, Th1 cells exhibit higher levels of aerobic glycolysis than Th2 cells. Moreover, Th1 cells are more sensitive to the inhibition of glycolysis, while higher concentrations of glycolysis inhibitor 2‐deoxyglucose are required to induce cell death in the Th2 lineage. These observations reveal that selection of metabolic pathways for substrate utilization during differentiation of Th1 and Th2 lineages is a fundamental process conserved across species.
IRON PROPHYLAXIS IN MENORRHAGIA
Authors: Arvidsson B. , Ekenved G. , Rybo G. , Sölvell L. 
Published: September 2024

Heavy menstrual bleedings frequently lead to iron deficiency. Iron suplementation is usually given to cover the increased losses. In the present study one tablet containing 100 mg of iron (DuroferonR, DurulesR) was given daily for 10 days in connection with the menstrual period in 15 women with menorrhagia. The tablets contained 59Felabelled FeSO4 and the total absorption was measured in a whole‐body counter. The mean menstrual blood loss was 117 ml (range 46–259 ml), corresponding to 53 mg of iron (range 21–117 mg). The mean absorption was 81 mg (range 19–145 mg). Individually, 14 of 15 subjects absorbed more iron from the tablets than was lost by the menstrual bleedings. Thus, the iron prophylaxis as applied in the present study seems to be sufficient in most women with menorrhagia.
Hdac3 deficiency limits periosteal reaction associated with Western diet feeding in female mice
Authors: Vu Elizabeth K. , Karkache Ismael Y. , Pham Anthony , Koroth Jinsha , Bradley Elizabeth W. 
Published: September 2024

AbstractDiet‐induced obesity is associated with enhanced systemic inflammation that limits bone regeneration. HDAC inhibitors are currently being explored as anti‐inflammatory agents. Prior reports show that myeloid progenitor‐directed Hdac3 ablation enhances intramembranous bone healing in female mice. In this study, we determined if Hdac3 ablation increased intramembranous bone regeneration in mice fed a high‐fat/high‐sugar (HFD) diet. Micro‐CT analyses demonstrated that HFD‐feeding enhanced the formation of periosteal reaction tissue of control littermates, reflective of suboptimal bone healing. We confirmed enhanced bone volume within the defect of Hdac3‐ablated females and showed that Hdac3 ablation reduced the amount of periosteal reaction tissue following HFD feeding. Osteoblasts cultured in a conditioned medium derived from Hdac3‐ablated cells exhibited a four‐fold increase in mineralization and enhanced osteogenic gene expression. We found that Hdac3 ablation elevated the secretion of several chemokines, including CCL2. We then confirmed that Hdac3 deficiency increased the expression of Ccl2. Lastly, we show that the proportion of CCL2‐positve cells within bone defects was significantly higher in Hdac3‐deficient mice and was further enhanced by HFD. Overall, our studies demonstrate that Hdac3 deletion enhances intramembranous bone healing in a setting of diet‐induced obesity, possibly through increased production of CCL2 by macrophages within the defect.
LONP1 alleviates ageing‐related renal fibrosis by maintaining mitochondrial homeostasis
Authors: Zhang Congxiao , Shen Siman , Xu Li , Li Man , Tian Binyao , Yao Li , Zhu Xinwang 
Published: September 2024

AbstractMitochondrial dysfunction is a pivotal event contributing to the development of ageing‐related kidney disorders. Lon protease 1 (LONP1) has been reported to be responsible for ageing‐related renal fibrosis; however, the underlying mechanism(s) of LONP1‐driven kidney ageing with respect to mitochondrial disturbances remains to be further explored. The level of LONP1 was tested in the kidneys of aged humans and mice. Renal fibrosis and mitochondrial quality control were confirmed in the kidneys of aged mice. Effects of LONP1 silencing or overexpression on renal fibrosis and mitochondrial quality control were explored. In addition, N6‐methyladenosine (m6A) modification and methyltransferase like 3 (METTL3) levels, the relationship between LONP1 and METTL3, and the impacts of METTL3 overexpression on mitochondrial functions were confirmed. Furthermore, the expression of insulin‐like growth factor 2 mRNA binding protein 2 (IGF2BP2) and the regulatory effects of IGF2BP2 on LONP1 were confirmed in vitro. LONP1 expression was reduced in the kidneys of aged humans and mice, accompanied by renal fibrosis and mitochondrial dysregulation. Overexpression of LONP1 alleviated renal fibrosis and maintained mitochondrial homeostasis, while silencing of LONP1 had the opposite effect. Impaired METTL3‐m6A signalling contributed at least in part to ageing‐induced LONP1 modification, reducing subsequent degradation in an IGF2BP2‐dependent manner. Moreover, METTL3 overexpression alleviated proximal tubule cell injury, preserved mitochondrial stability, inhibited LONP1 degradation, and protected mitochondrial functions. LONP1 mediates mitochondrial function in kidney ageing and that targeting LONP1 may be a potential therapeutic strategy for improving ageing‐related renal fibrosis.
Linking activation of synaptic NMDA receptors‐induced CREB signaling to brief exposure of cortical neurons to oligomeric amyloid‐beta peptide
Authors: Ferreira I. Luísa , Marinho Daniela , de Rosa Valéria , Castanheira Bárbara , Fang Zongwei , Caldeira Gladys L. , Mota Sandra I. , Rego A. Cristina 
Published: September 2024

AbstractAmyloid‐beta peptide oligomers (AβO) have been considered “primum movens” for a cascade of events that ultimately cause selective neuronal death in Alzheimer's disease (AD). However, initial events triggered by AβO have not been clearly defined. Synaptic (Syn) N‐methyl‐d‐aspartate receptors (NMDAR) are known to activate cAMP response element‐binding protein (CREB), a transcriptional factor involved in gene expression related to cell survival, memory formation and synaptic plasticity, whereas activation of extrasynaptic (ESyn) NMDARs was linked to excitotoxic events. In AD brain, CREB phosphorylation/activation was shown to be altered, along with dyshomeostasis of intracellular Ca2+ (Ca2+i). Thus, in this work, we analyze acute/early and long‐term AβO‐mediated changes in CREB activation involving Syn or ESyn NMDARs in mature rat cortical neurons. Our findings show that acute AβO exposure produce early increase in phosphorylated CREB, reflecting CREB activity, in a process occurring through Syn NMDAR‐mediated Ca2+ influx. Data also demonstrate that AβO long‐term (24 h) exposure compromises synaptic function related to Ca2+‐dependent CREB phosphorylation/activation and nuclear CREB levels and related target genes, namely Bdnf, Gadd45γ, and Btg2. Data suggest a dual effect of AβO following early or prolonged exposure in mature cortical neurons through the activation of the CREB signaling pathway, linked to the activation of Syn NMDARs.
image
Supervisor‐directed anger as a link between work–family conflict and unethical pro‐family behaviours: An attributional perspective
Authors: Wu Lusi , Perrigino Matthew B. 
Published: September 2024

AbstractResearch explores how internal‐focused cognitions and emotions impact the connection between employees' work–family conflict (WFC) and subsequent behaviours. We offer a complementary view by arguing that employees also attribute WFC to external causes, notably their supervisor. First, we hypothesize that anger directed towards one's supervisor mediates the relationship between WFC and unethical pro‐family behaviours (UPFB), which is supported by the results of a multi‐wave survey study. Second, we expand this view by recognizing employees' experiences of WFC may be beyond the supervisor's control. We examine how the extent to which the employee's WFC is perceived as more (vs. less) controllable by their supervisor conditions this indirect effect. Results from an experimental study show that when WFC is perceived as more controllable by one's supervisor, the positive association between WFC and anger is stronger, reinforcing the indirect effect of WFC on UPFB. However, when WFC is perceived as less controllable by one's supervisor, the indirect effect disappears as anger towards the supervisor dissipates. Taken together, our work synthesizes the work–family and UPFB literatures by addressing the key roles of anger and external attributions in the experience of WFC.
Non‐native plants tend to be phylogenetically distant but functionally similar to native plants under intense disturbance at the Three Gorges Reservoir Area
Authors: Le Haichuan , Mao Jiangtao , Cavender‐Bares Jeannine , Pinto‐Ledezma Jesús N. , Deng Ying , Zhao Changming , Xiong Gaoming , Xu Wenting , Xie Zongqiang 
Published: September 2024

Summary

Darwin's two opposing hypotheses, proposing that non‐native species closely or distantly related to native species are more likely to succeed, are known as ‘Darwin's Naturalization Conundrum’. Recently, invasion ecologists have sought to unravel these hypotheses. Studies that incorporate rich observational data in disturbed ecosystems that integrate phylogenetic and functional perspectives have potential to shed light on the conundrum.

Using 313 invaded plant communities including 46 invasive plant species and 531 native plant species across the Three Gorges Reservoir Area in China, we aim to evaluate the coexistence mechanisms of invasive and native plants by integrating phylogenetic and functional dimensions at spatial and temporal scales.

Our findings revealed that invasive plants tended to co‐occur more frequently with native plant species that were phylogenetically distant but functionally similar in the reservoir riparian zone. Furthermore, our study demonstrated that the filtering of flood‐dry‐flood cycles played a significant role in deepening functional similarities of native communities and invasive‐native species over time.

Our study highlights the contrasting effects of phylogenetic relatedness and functional similarity between invasive and native species in highly flood‐disturbed habitats, providing new sights into Darwin's Naturalization Conundrum.
Topology and functional characterization of major outer membrane proteins of Treponema maltophilum and Treponema lecithinolyticum
Authors: Anselmi Natalie K. , Vanyo Stephen T. , Clark Nicholas D. , Rodriguez Dayron M. Leyva , Jones Megan M. , Rosenthal Sara , Patel Dhara , Marconi Richard T. , Visser Michelle B. 
Published: September 2024

AbstractNumerous Treponema species are prevalent in the dysbiotic subgingival microbial community during periodontitis. The major outer sheath protein is a highly expressed virulence factor of the well‐characterized species Treponema denticola. Msp forms an oligomeric membrane protein complex with adhesin and porin properties and contributes to host–microbial interaction. Treponema maltophilum and Treponema lecithinolyticum species are also prominent during periodontitis but are relatively understudied. Msp‐like membrane surface proteins exist in T. maltophilum (MspA) and T. lecithinolyticum (MspTL), but limited information exists regarding their structural features or functionality. Protein profiling reveals numerous differences between these species, but minimal differences between strains of the same species. Using protein modeling tools, we predict MspA and MspTL monomeric forms to be large β‐barrel structures composed of 20 all‐next‐neighbor antiparallel β strands which most likely adopt a homotrimer formation. Using cell fractionation, Triton X‐114 phase partitioning, heat modifiability, and chemical and detergent release assays, we found evidence of amphiphilic integral membrane‐associated oligomerization for both native MspA and MspTL in intact spirochetes. Proteinase K accessibility and immunofluorescence assays demonstrate surface exposure of MspA and MspTL. Functionally, purified recombinant MspA or MspTL monomer proteins can impair neutrophil chemotaxis. Expressions of MspA or MspTL with a PelB leader sequence in Escherichia coli also demonstrate surface exposure and can impair neutrophil chemotaxis in an in vivo air pouch model of inflammation. Collectively, our data demonstrate that MspA and MspTL membrane proteins can contribute to pathogenesis of these understudied oral spirochete species.