bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voice More and Stay Longer: How Ethical Leaders Influence Employee Voice and Exit Intentions
Authors: Lam Long W. , Loi Raymond , Chan Ka Wai , Liu Yan 
Published: May 2016

ABSTRACT:Given the importance of voice in ethical leadership theory, we analyze the relationship of ethical leadership to employee voice and the relationship of voice to exit intentions. Building on the theory of work engagement, we further hypothesize that cognitive engagement mediates these proposed relationships. To test these propositions, we conduct a field study to relate ethical leadership of supervisors, measured at time 1, to employees’ cognitive job engagement, measured at time 2. The analyses show that the relationship between these variables can account for supervisory ethical leadership’s association with employee voice and exit intentions. In a supplementary study using a different sample, we find that supervisory ethical leadership is related to exit intentions through voice. We discuss how these findings contribute to the literature on ethical leadership, employee voice, and exit.
Assessment of Groin Application of Junctional Tourniquets in a Manikin Model
Authors: Kragh John F. , Lunati Matthew P. , Kharod Chetan U. , Cunningham Cord W. , Bailey Jeffrey A. , Stockinger Zsolt T. , Cap Andrew P. , Chen Jacob , Aden James K. , Cancio Leopoldo C. 
Published: May 2016

AbstractIntroductionTo aid in preparation of military medic trainers for a possible new curriculum in teaching junctional tourniquet use, the investigators studied the time to control hemorrhage and blood volume lost in order to provide evidence for ease of use.HypothesisModels of junctional tourniquet could perform differentially by blood loss, time to hemostasis, and user preference.MethodsIn a laboratory experiment, 30 users controlled simulated hemorrhage from a manikin (Combat Ready Clamp [CRoC] Trainer) with three iterations each of three junctional tourniquets. There were 270 tests which included hemorrhage control (yes/no), time to hemostasis, and blood volume lost. Users also subjectively ranked tourniquet performance. Models included CRoC, Junctional Emergency Treatment Tool (JETT), and SAM Junctional Tourniquet (SJT). Time to hemostasis and total blood loss were log-transformed and analyzed using a mixed model analysis of variance (ANOVA) with the users represented as random effects and the tourniquet model used as the treatment effect. Preference scores were analyzed with ANOVA, and Tukey’s honest significant difference test was used for all post-hoc pairwise comparisons.ResultsAll tourniquet uses were 100% effective for hemorrhage control. For blood loss, CRoC and SJT performed best with least blood loss and were significantly better than JETT; in pairwise comparison, CRoC-JETT (P< .0001) and SJT-JETT (P= .0085) were statistically significant in their mean difference, while CRoC-SJT (P= .35) was not. For time to hemostasis in pairwise comparison, the CRoC had a significantly shorter time compared to JETT and SJT (P< .0001, both comparisons); SJT-JETT was also significant (P= .0087). In responding to the directive, “Rank the performance of the models from best to worst,” users did not prefer junctional tourniquet models differently (P> .5, all models).ConclusionThe CRoC and SJT performed best in having least blood loss, CRoC performed best in having least time to hemostasis, and users did not differ in preference of model. Models of junctional tourniquet performed differentially by blood loss and time to hemostasis.KraghJFJr,LunatiMP,KharodCU,CunninghamCW,BaileyJA,StockingerZT,CapAP,ChenJ,AdenJK3d,CancioLC.Assessment of groin application of junctional tourniquets in a manikin model.Prehosp Disaster Med.2016;31(4):358–363.
Mapping the electrostatic force field of single molecules from high-resolution scanning probe images
Authors: Hapala Prokop , Švec Martin , Stetsovych Oleksandr , van der Heijden Nadine J. , Ondráček Martin , van der Lit Joost , Mutombo Pingo , Swart Ingmar , Jelínek Pavel 
Published: May 2016

AbstractHow electronic charge is distributed over a molecule determines to a large extent its chemical properties. Here, we demonstrate how the electrostatic force field, originating from the inhomogeneous charge distribution in a molecule, can be measured with submolecular resolution. We exploit the fact that distortions typically observed in high-resolution atomic force microscopy images are for a significant part caused by the electrostatic force acting between charges of the tip and the molecule of interest. By finding a geometrical transformation between two high-resolution AFM images acquired with two different tips, the electrostatic force field or potential over individual molecules and self-assemblies thereof can be reconstructed with submolecular resolution.
ATP6AP1 deficiency causes an immunodeficiency with hepatopathy, cognitive impairment and abnormal protein glycosylation
Authors: Jansen Eric J. R. , Timal Sharita , Ryan Margret , Ashikov Angel , van Scherpenzeel Monique , Graham Laurie A. , Mandel Hanna , Hoischen Alexander , Iancu Theodore C. , Raymond Kimiyo , Steenbergen Gerry , Gilissen Christian , Huijben Karin , van Bakel Nick H. M. , Maeda Yusuke , Rodenburg Richard J. , Adamowicz Maciej , Crushell Ellen , Koenen Hans , Adams Darius , Vodopiutz Julia , Greber-Platzer Susanne , Müller Thomas , Dueckers Gregor , Morava Eva , Sykut-Cegielska Jolanta , Martens Gerard J. M. , Wevers Ron A. , Niehues Tim , Huynen Martijn A. , Veltman Joris A. , Stevens Tom H. , Lefeber Dirk J. 
Published: May 2016

AbstractThe V-ATPase is the main regulator of intra-organellar acidification. Assembly of this complex has extensively been studied in yeast, while limited knowledge exists for man. We identified 11 male patients with hemizygous missense mutations in ATP6AP1, encoding accessory protein Ac45 of the V-ATPase. Homology detection at the level of sequence profiles indicated Ac45 as the long-sought human homologue of yeast V-ATPase assembly factor Voa1. Processed wild-type Ac45, but not its disease mutants, restored V-ATPase-dependent growth in Voa1 mutant yeast. Patients display an immunodeficiency phenotype associated with hypogammaglobulinemia, hepatopathy and a spectrum of neurocognitive abnormalities. Ac45 in human brain is present as the common, processed ∼40-kDa form, while liver shows a 62-kDa intact protein, and B-cells a 50-kDa isoform. Our work unmasks Ac45 as the functional ortholog of yeast V-ATPase assembly factor Voa1 and reveals a novel link of tissue-specific V-ATPase assembly with immunoglobulin production and cognitive function.
Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism–dystonia
Authors: Tuschl Karin , Meyer Esther , Valdivia Leonardo E. , Zhao Ningning , Dadswell Chris , Abdul-Sada Alaa , Hung Christina Y. , Simpson Michael A. , Chong W. K. , Jacques Thomas S. , Woltjer Randy L. , Eaton Simon , Gregory Allison , Sanford Lynn , Kara Eleanna , Houlden Henry , Cuno Stephan M. , Prokisch Holger , Valletta Lorella , Tiranti Valeria , Younis Rasha , Maher Eamonn R. , Spencer John , Straatman-Iwanowska Ania , Gissen Paul , Selim Laila A. M. , Pintos-Morell Guillem , Coroleu-Lletget Wifredo , Mohammad Shekeeb S. , Yoganathan Sangeetha , Dale Russell C. , Thomas Maya , Rihel Jason , Bodamer Olaf A. , Enns Caroline A. , Hayflick Susan J. , Clayton Peter T. , Mills Philippa B. , Kurian Manju A. , Wilson Stephen W. 
Published: May 2016

AbstractAlthough manganese is an essential trace metal, little is known about its transport and homeostatic regulation. Here we have identified a cohort of patients with a novel autosomal recessive manganese transporter defect caused by mutations in SLC39A14. Excessive accumulation of manganese in these patients results in rapidly progressive childhood-onset parkinsonism–dystonia with distinctive brain magnetic resonance imaging appearances and neurodegenerative features on post-mortem examination. We show that mutations in SLC39A14 impair manganese transport in vitro and lead to manganese dyshomeostasis and altered locomotor activity in zebrafish with CRISPR-induced slc39a14 null mutations. Chelation with disodium calcium edetate lowers blood manganese levels in patients and can lead to striking clinical improvement. Our results demonstrate that SLC39A14 functions as a pivotal manganese transporter in vertebrates.
A method to rapidly create protein aggregates in living cells
Authors: Miyazaki Yusuke , Mizumoto Kota , Dey Gautam , Kudo Takamasa , Perrino John , Chen Ling-chun , Meyer Tobias , Wandless Thomas J. 
Published: May 2016

AbstractThe accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.
A dual molecular analogue tuner for dissecting protein function in mammalian cells
Authors: Brosh Ran , Hrynyk Iryna , Shen Jessalyn , Waghray Avinash , Zheng Ning , Lemischka Ihor R. 
Published: May 2016

AbstractLoss-of-function studies are fundamental for dissecting gene function. Yet, methods to rapidly and effectively perturb genes in mammalian cells, and particularly in stem cells, are scarce. Here we present a system for simultaneous conditional regulation of two different proteins in the same mammalian cell. This system harnesses the plant auxin and jasmonate hormone-induced degradation pathways, and is deliverable with only two lentiviral vectors. It combines RNAi-mediated silencing of two endogenous proteins with the expression of two exogenous proteins whose degradation is induced by external ligands in a rapid, reversible, titratable and independent manner. By engineering molecular tuners for NANOG, CHK1, p53 and NOTCH1 in mammalian stem cells, we have validated the applicability of the system and demonstrated its potential to unravel complex biological processes.
Biology and survival of extremely halophilic archaeon Haloarcula marismortui RR12 isolated from Mumbai salterns, India in response to salinity stress
Authors: Thombre Rebecca S. , Shinde Vinaya D. , Oke Radhika S. , Dhar Sunil Kumar , Shouche Yogesh S. 
Published: May 2016

AbstractHaloarchaea are unique microorganism’s resistant to environmental and osmotic stresses and thrive in their habitats despite extreme fluctuating salinities. In the present study, haloarchaea were isolated from hypersaline thalossohaline salterns of Bhandup, Mumbai, India and were identified as Haloferax prahovense, Haloferax alexandrines, Haloferax lucentense, Haloarcula tradensis, Haloarcula marismortui and Haloarcula argentinensis. The mechanism of adaptation to contrasting salinities (1.5 M and 4.5 M) was investigated in the extreme haloarchaeon, Hal. marismortui RR12. Hal. marismortui RR12 increased the intracellular sequestration of K+ and Cl− ions in hypo salinity and hyper salinity respectively as detected by Energy-dispersive X-ray spectroscopy microanalysis (EDAX) and Inductively Coupled Plasma- atomic Emission Spectroscopy (ICP-AES) indicating the presence of ‘salt-in’ strategy of osmoadaptation. As a cellular response to salinity stress, it produced small heat shock like proteins (sHSP) identified using MALDI-TOF MS and increased the production of protective red carotenoid pigment. This is the first report on the study of the concomitant cellular, molecular and physiological mechanism adapted by Hal. marismortui RR12 when exposed to contrasting salinities in external environment.
ICOS+PD-1+CXCR3+ T follicular helper cells contribute to the generation of high-avidity antibodies following influenza vaccination
Authors: Bentebibel Salah-Eddine , Khurana Surender , Schmitt Nathalie , Kurup Parvathi , Mueller Cynthia , Obermoser Gerlinde , Palucka A. Karolina , Albrecht Randy A. , Garcia-Sastre Adolfo , Golding Hana , Ueno Hideki 
Published: May 2016

AbstractThe immune mechanism leading to the generation of protective antibody responses following influenza trivalent inactivated vaccine (TIV) vaccinations remains largely uncharacterized. We recently reported that TIV vaccination induced a transient increase of circulating ICOS+PD-1+CXCR3+ T follicular helper (cTfh) cells in blood, which positively correlated with the induction of protective antibody responses measured at day 28. However, whether and how these T cells directly contribute to antibody response remains unclear. In this study, we analyzed the changes after TIV vaccination in the amount and the avidity of the polyclonal antibodies specific for the HA1 subunit of the pandemic H1N1 virus, and analyzed the correlation with the increase of ICOS+PD-1+CXCR3+ cTfh cells. We found that both the amount and the avidity of specific antibodies rapidly increased during the first 7 days after TIV. Importantly, the increase of ICOS+PD-1+CXCR3+ cTfh cells strongly correlated with the increase in the avidity of antibodies, particularly in subjects who did not have high affinity antibodies at baseline. We propose that ICOS+PD-1+CXCR3+ Tfh cells directly contribute to the generation of high-avidity antibodies after TIV vaccinations by selectively interacting with high affinity B cells at extrafollicular sites.
Multiscale architectured materials with composition and grain size gradients manufactured using high-pressure torsion
Authors: Kang Ji Yun , Kim Jung Gi , Park Hyo Wook , Kim Hyoung Seop 
Published: May 2016

AbstractThe concept of multiscale architectured materials is established using composition and grain size gradients. Composition-gradient nanostructured materials are produced from coarse grained interstitial free steels via carburization and high-pressure torsion. Quantitative analyses of the dislocation density using X-ray diffraction and microstructural studies clearly demonstrate the gradients of the dislocation density and grain size. The mechanical properties of the gradient materials are compared with homogeneous nanostructured carbon steel without a composition gradient in an effort to investigate the gradient effect. Based on the above observations, the potential of multiscale architecturing to open a new material property is discussed.
New insights into the transport processes controlling the sulfate-methane-transition-zone near methane vents
Authors: Sultan Nabil , Garziglia Sébastien , Ruffine Livio 
Published: May 2016

AbstractOver the past years, several studies have raised concerns about the possible interactions between methane hydrate decomposition and external change. To carry out such an investigation, it is essential to characterize the baseline dynamics of gas hydrate systems related to natural geological and sedimentary processes. This is usually treated through the analysis of sulfate-reduction coupled to anaerobic oxidation of methane (AOM). Here, we model sulfate reduction coupled with AOM as a two-dimensional (2D) problem including, advective and diffusive transport. This is applied to a case study from a deep-water site off Nigeria’s coast where lateral methane advection through turbidite layers was suspected. We show by analyzing the acquired data in combination with computational modeling that a two-dimensional approach is able to accurately describe the recent past dynamics of such a complex natural system. Our results show that the sulfate-methane-transition-zone (SMTZ) is not a vertical barrier for dissolved sulfate and methane. We also show that such a modeling is able to assess short timescale variations in the order of decades to centuries.
Population genetic structure and post-LGM expansion of the plant bug Nesidiocoris tenuis (Hemiptera: Miridae) in China
Authors: Xun Huaizhu , Li Hu , Li Shujuan , Wei Shujun , Zhang Lijuan , Song Fan , Jiang Pei , Yang Hailin , Han Fei , Cai Wanzhi 
Published: May 2016

AbstractThe plant bug,Nesidiocoris tenuis(Hemiptera: Miridae), is one of the most thermophilous dicyphines in agroecosystems and is widely distributed in China. Little is known regarding the genetic structure ofN. tenuisand the effect of historical climatic fluctuations onN. tenuispopulations. We analyzed partial sequences of three mitochondrial protein-coding genes (COI, ND2andCytB) and nuclear genes (5.8S, ITS2 and28S) for 516 specimens collected from 37 localities across China. Analyses of the combined mitochondrial dataset indicated that the Southwestern China group (SWC) was significantly differentiated from the remaining populations, other Chinese group (OC). Asymmetric migration and high level of gene flow across a long distance within the OC group was detected. The long-distance dispersal ofN. tenuismight be affected by air currents and human interference. Both the neutrality tests and mismatch distributions revealed the occurrence of historical population expansion. Bayesian skyline plot analyses with two different substitution rates indicated thatN. tenuismight follow the post-LGM (the Last Glacial Maximum) expansion pattern for temperate species. Pleistocene climatic fluctuation, complicated topography and anthropogenic factors, along with other ecological factors (e.g. temperature and air current) might have accounted for the current population structure ofN. tenuis.
Dependences of Q-branch integrated intensity of linear-molecule pendular spectra on electric-field strength and rotational temperature and its potential applications
Authors: Deng Min , Wang Hailing , Wang Qin , Yin Jianping 
Published: May 2016

AbstractWe calculate the pendular-state spectra of cold linear molecules, and investigated the dependences of “Q-branch” integrated intensity of pendular spectra on both electric-field strength and molecular rotation-temperature. A new multi-peak structure in the “Q-branch” spectrum is appearing when the Stark interaction strength ω = μE/B equal to or larger than the critical value. Our study shows that the above results can be used not only to measure the electric-field vector and its spatial distribution in some electrostatic devices, such as the Stark decelerator, Stark velocity filter and electrostatic trap and so on, but also to survey the orientation degree of cold linear molecules in a strong electrostatic field.
RhBMP-2 Activates Hippo Signaling through RASSF1 in Esophageal Cancer Cells
Authors: Kim Soo Mi , Ye Shuai , Rah So-Young , Park Byung Hyun , Wang Hongen , Kim Jung-Ryul , Kim Seung Ho , Jang Kyu Yun , Lee Kwang-Bok 
Published: May 2016

AbstractDespite that recombinant human bone morphogenetic protein-2 (rhBMP-2) has been reported as a stimulatory effecter of cancer cell growth because of its characteristic like morphogen, the biological functions of rhBMP-2 in human esophageal cancer cells are unknown. The purpose of this study was to investigate whether rhBMP-2 has an inhibitory effect on the growth of human esophageal squamous carcinoma cells (ESCC). RhBMP-2 significantly inhibited proliferation of ESCC cells in a dose-dependent manner in the MTT assay. Cell cycle arrest at the G1 phase was induced 24 h after rhBMP2 treatment. RhBMP-2 also reduced cyclin D1, cyclin-dependent kinase (CDK) 4 and CDK 6 activities, and stimulated p-Smad1/5/8, p53, and p21 levels at 12 h. In contrast, rhBMP-2 diminished poly (ADP-ribose) polymerase (PARP) protein expression levels and activated cleaved PARP, cleaved caspase-7, and cleaved-caspase 9 levels in ESCC cells. In addition, rhBMP-2 increased MST1, MOB1, and p-YAP protein levels and the RASSF1 binds Mst1 more upon treatment with rhBMP2. The induced p-YAP expression in TE-8 and TE-12 cells by rhBMP-2 was reversed by the RASSF1 knockdown. In vivo study, rhBMP-2 decreased tumor volume following subcutaneous implantation and showed higher radiologic score (less bony destruction) after femoral implantation compared to those in a control group. These results suggest that rhBMP-2 inhibits rather than activates proliferation of human esophageal cancer cells which is mediated through activating the hippo signaling pathway.
Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB
Authors: Haustead Daniel J. , Stevenson Andrew , Saxena Vishal , Marriage Fiona , Firth Martin , Silla Robyn , Martin Lisa , Adcroft Katharine F. , Rea Suzanne , Day Philip J. , Melton Phillip , Wood Fiona M. , Fear Mark W. 
Published: May 2016

AbstractAge is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through meta-analysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging.
Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine
Authors: Kopp Sascha , Slumstrup Lasse , Corydon Thomas J. , Sahana Jayashree , Aleshcheva Ganna , Islam Tawhidul , Magnusson Nils E. , Wehland Markus , Bauer Johann , Infanger Manfred , Grimm Daniela 
Published: May 2016

AbstractMany cell types form three-dimensional aggregates (MCS; multicellular spheroids), when they are cultured under microgravity. MCS often resemble the organ, from which the cells have been derived. In this study we investigated human MCF-7 breast cancer cells after a 2 h-, 4 h-, 16 h-, 24 h- and 5d-exposure to a Random Positioning Machine (RPM) simulating microgravity. At 24 h few small compact MCS were detectable, whereas after 5d many MCS were floating in the supernatant above the cells, remaining adherently (AD). The MCS resembled the ducts formed in vivo by human epithelial breast cells. In order to clarify the underlying mechanisms, we harvested MCS and AD cells separately from each RPM-culture and measured the expression of 29 selected genes with a known involvement in MCS formation. qPCR analyses indicated that cytoskeletal genes were unaltered in short-term samples. IL8, VEGFA and FLT1 were upregulated in 2 h/4 h AD-cultures. The ACTB, TUBB, EZR, RDX, FN1, VEGFA, FLK1 Casp9, Casp3, PRKCA mRNAs were downregulated in 5d-MCS-samples. ESR1 was upregulated in AD and PGR1 in both phenotypes after 5d. A pathway analysis revealed that the corresponding gene products are involved in organization and regulation of the cell shape, in cell tip formation and membrane to membrane docking.