bluuebunny/crossref_metadata_2025_split huggingface dataset:
Record dry summer in 2015 challenges precipitation projections in Central Europe
Authors: Orth René , Zscheischler Jakob , Seneviratne Sonia I. 
Published: June 2016

AbstractCentral Europe was characterized by a humid-temperate climate in the 20th century. Climate change projections suggest that climate in this area will shift towards warmer temperatures by the end of the 21st century, while projected precipitation changes are highly uncertain. Here we show that the 2015 summer rainfall was the lowest on record since 1901 in Central Europe and that climate models that perform best in the three driest years of the historical time period 1901–2015 project stronger drying trends in the 21st century than models that perform best in the remaining years. Analyses of precipitation and derived soil moisture reveal that the 2015 event was drier than both the recent 2003 or 2010 extreme summers in Central Europe. Additionally there are large anomalies in satellite-derived vegetation greenness. In terms of precipitation and temperature anomalies, the 2015 summer in Central Europe is found to lie between historical climate in the region and that characteristic of the Mediterranean area. Even though the models best capturing past droughts are not necessarily generally more reliable in the future, the 2015 drought event illustrates that potential future drying trends have severe implications and could be stronger than commonly assumed from the entire IPCC AR5 model ensemble.
Enhanced Ghrelin Levels and Hypothalamic Orexigenic AgRP and NPY Neuropeptide Expression in Models of Jejuno-Colonic Short Bowel Syndrome
Authors: Gillard Laura , Billiauws Lore , Stan-Iuga Bogdan , Ribeiro-Parenti Lara , Jarry Anne-Charlotte , Cavin Jean-Baptiste , Cluzeaud Françoise , Mayeur Camille , Thomas Muriel , Freund Jean-Noël , Lacorte Jean-Marc , Le Gall Maude , Bado André , Joly Francisca , Le Beyec Johanne 
Published: June 2016

AbstractShort bowel syndrome (SBS) patients developing hyperphagia have a better outcome. Gastrointestinal endocrine adaptations help to improve intestinal functions and food behaviour. We investigated neuroendocrine adaptations in SBS patients and rat models with jejuno-ileal (IR-JI) or jejuno-colonic (IR-JC) anastomosis with and without parenteral nutrition. Circulating levels of ghrelin, PYY, GLP-1, and GLP-2 were determined in SBS rat models and patients. Levels of mRNA for proglucagon, PYY and for hypothalamic neuropeptides were quantified by qRT-PCR in SBS rat models. Histology and immunostaining for Ki67, GLP-1 and PYY were performed in SBS rats. IR-JC rats, but not IR-JI, exhibited significantly higher crypt depths and number of Ki67-positive cells than sham. Fasting and/or postprandial plasma ghrelin and PYY concentrations were higher, or tend to be higher, in IR-JC rats and SBS-JC patients than in controls. Proglucagon and Pyy mRNA levels were significantly enhanced in IR-JC rats. Levels of mRNA coding hypothalamic orexigenic NPY and AgRP peptides were significantly higher in IR-JC than in sham rats. We demonstrate an increase of plasma ghrelin concentrations, major changes in hypothalamic neuropeptides levels and greater induction of PYY in SBS-JC rats and patients suggesting that jejuno-colonic continuity creates a peculiar environment promoting further gut-brain adaptations.
Water-soluble benzylidene cyclopentanone based photosensitizers for in vitro and in vivo antimicrobial photodynamic therapy
Authors: Fang Yanyan , Liu Tianlong , Zou Qianli , Zhao Yuxia , Wu Feipeng 
Published: June 2016

AbstractAntimicrobial photodynamic therapy (aPDT) has been proposed to cope with the increasing antibiotic resistance among pathogens. As versatile pharmacophores, benzylidene cyclopentanone based photosensitizers (PSs) have been used in various bioactive materials. However, their reports as aPDT agents are very limited, and relationships between their chemical structures and antibacterial abilities have not been systematically discussed. Here, nine water-soluble benzylidene cyclopentanone PSs modified by polyethylene glycol (PEG), carboxylate anionic or pyridyl cationic agents are studied for aPDT. It is found that the binding/uptake abilities and aPDT effects of these PSs toward bacterial cells vary significantly when adjusting the number and position of their terminal charged groups. Though the comparable (also best) binding/uptake amounts are achieved by both cationic PS P3 and anionic PS Y1, only Y1 exhibits much more excellent aPDT activities than other PSs. Antibacterial mechanisms reveal that, relative to the favorable cell wall-binding of cationic PS P3, the anionic PS Y1 can accumulate more in the spheroplast/protoplast of methicillin-resistant Staphylococcus aureus (MRSA), which ensures its high efficient aPDT abilities both in vitro and in vivo. This study suggests the great clinical application potential of Y1 in inactivation of MRSA.
An interpolatory ansatz captures the physics of one-dimensional confined Fermi systems
Authors: Andersen M. E. S. , Dehkharghani A. S. , Volosniev A. G. , Lindgren E. J. , Zinner N. T. 
Published: June 2016

AbstractInteracting one-dimensional quantum systems play a pivotal role in physics. Exact solutions can be obtained for the homogeneous case using the Bethe ansatz and bosonisation techniques. However, these approaches are not applicable when external confinement is present. Recent theoretical advances beyond the Bethe ansatz and bosonisation allow us to predict the behaviour of one-dimensional confined systems with strong short-range interactions and new experiments with cold atomic Fermi gases have already confirmed these theories. Here we demonstrate that a simple linear combination of the strongly interacting solution with the well-known solution in the limit of vanishing interactions provides a simple and accurate description of the system for all values of the interaction strength. This indicates that one can indeed capture the physics of confined one-dimensional systems by knowledge of the limits using wave functions that are much easier to handle than the output of typical numerical approaches. We demonstrate our scheme for experimentally relevant systems with up to six particles. Moreover, we show that our method works also in the case of mixed systems of particles with different masses. This is an important feature because these systems are known to be non-integrable and thus not solvable by the Bethe ansatz technique.
Electrically switchable organo–inorganic hybrid for a white-light laser source
Authors: Huang Jui-Chieh , Hsiao Yu-Cheng , Lin Yu-Ting , Lee Chia-Rong , Lee Wei 
Published: June 2016

AbstractWe demonstrate a spectrally discrete white-light laser device based on a photonic bandgap hybrid, which is composed of a soft photonic crystal; i.e., a layer of dye-doped cholesteric liquid crystal (CLC), sandwiched between two imperfect but identical, inorganic multilayer photonic crystals. With a sole optical pump, a mono-, bi-, or tri-chromatic laser can be obtained and, through the soft photonic crystal regulated by an applied voltage, the hybrid possesses electrical tunability in laser wavelength. The three emitted spectral peaks originate from two bandedges of the CLC reflection band as well as one of the photonic defect modes in dual-mode lasing. Thanks to the optically bistable nature of CLC, such a white-light laser device can operate in quite an energy-saving fashion. This technique has potential to fulfill the present mainstream in the coherent white-light source.
Soil fungal community comparison of different mulberry genotypes and the relationship with mulberry fruit sclerotiniosis
Authors: Yu Cui , Hu Xingming , Deng Wen , Li Yong , Han Guangming , Ye Chuhua 
Published: June 2016

AbstractMulberry fruit sclerotiniosis is a common soil-borne disease. We hypothesize that there is a relationship between the incidence of mulberry fruit sclerotiniosis and the soil fungal community. Therefore four mulberry genotypes with different resistance to sclerotiniosis were selected to study their soil fungal community under the same condition. A total of 6 phyla and 93 genera were identified from all mulberry samples. Genera affected by genotype, cover, and genotype by cover interactions, were 23, 20, and 11, respectively. There were 10 genera that differed between the resistant cultivars and the susceptible cultivars. In addition, the relative abundances of Humicola and Mortierella in the resistant mulberry cultivars with the cover treatments were significantly higher than that of in the susceptible cultivars. However, the relative abundance of Sclerotiniaceae and Scleromitrula that may cause sclerotiniosis in the uncover treatments were significantly higher compared with the cover treatments. The results suggested that the genotype of mulberry has a significant impact on the soil microbial community that may result in differences in resistance to sclerotiniosis, and covering could reduce the relative abundance of Sclerotiniaceae or Scleromitrula.
The impact of simulated MRI scanner background noise on visual attention processes as measured by the EEG
Authors: Kobald S. Oliver , Getzmann Stephan , Beste Christian , Wascher Edmund 
Published: June 2016

AbstractEnvironmental noise is known to affect personal well-being as well as cognitive processes. Besides daily life, environmental noise can also occur in experimental research settings, e.g. when being in a magnetic resonance scanner. Scanner background noise (SBN) might pose serious confounds for experimental findings, even when non-auditory settings are examined. In the current experiment we tested if SBN alters bottom-up and top-down related processes of selective visual attention mechanisms. Participants completed two blocks of a visual change detection task, one block in silence and one block under SBN exposure. SBN was found to decrease accuracy in measures of visual attention. This effect was modulated by the temporal occurrence of SBN. When SBN was encountered in the first block, it prevented a significant improvement of accuracy in the second block. When SBN appeared in the second block, it significantly decreased accuracy. Neurophysiological findings showed a strong frontal positivity shift only when SBN was present in the first block, suggesting an inhibitory process to counteract the interfering SBN. Common correlates of both top-down and bottom-up processes of selective visual attention were not specifically affected by SBN exposure. Further research appears necessary to entirely rule out confounds of SBN in assessing visual attention.
Dual Drug Loaded Biodegradable Nanofibrous Microsphere for Improving Anti-Colon Cancer Activity
Authors: Fan Rangrang , Li Xiaoling , Deng Jiaojiao , Gao Xiang , Zhou Liangxue , Zheng Yu , Tong Aiping , Zhang Xiaoning , You Chao , Guo Gang 
Published: June 2016

AbstractOne of the approaches being explored to increase antitumor activity of chemotherapeutics is to inject drug-loaded microspheres locally to specific anatomic sites, providing for a slow, long term release of a chemotherapeutic while minimizing systemic exposure. However, the used clinically drug carriers available at present have limitations, such as their low stability, renal clearance and residual surfactant. Here, we report docetaxel (DOC) and curcumin (CUR) loaded nanofibrous microspheres (DOC + CUR/nanofibrous microspheres), self-assembled from biodegradable PLA-PEO-PPO-PEO-PLA polymers as an injectable drug carrier without adding surfactant during the emulsification process. The obtained nanofibrous microspheres are composed entirely of nanofibers and have an open hole on the shell without the assistance of a template. It was shown that these DOC + CUR/nanofibrous microspheres could release curcumin and docetaxel slowly in vitro. The slow, sustained release of curcumin and docetaxel in vivo may help maintain local concentrations of active drug. The mechanism by which DOC + CUR/nanofibrous microspheres inhibit colorectal peritoneal carcinomatosis might involve increased induction of apoptosis in tumor cells and inhibition of tumor angiogenesis. In vitro and in vivo evaluations demonstrated efficacious synergistic antitumor effects against CT26 of curcumin and docetaxel combined nanofibrous microspheres. In conclusion, the dual drug loaded nanofibrous microspheres were considered potentially useful for treating abdominal metastases of colorectal cancer.
Altered multisensory temporal integration in obesity
Authors: Scarpina Federica , Migliorati Daniele , Marzullo Paolo , Mauro Alessandro , Scacchi Massimo , Costantini Marcello 
Published: June 2016

AbstractEating is a multisensory behavior. The act of placing food in the mouth provides us with a variety of sensory information, including gustatory, olfactory, somatosensory, visual and auditory. Evidence suggests altered eating behavior in obesity. Nonetheless, multisensory integration in obesity has been scantily investigated so far. Starting from this gap in the literature, we seek to provide the first comprehensive investigation of multisensory integration in obesity. Twenty male obese participants and twenty male healthy-weight participants took part in the study aimed at describing the multisensory temporal binding window (TBW). The TBW is defined as the range of stimulus onset asynchrony in which multiple sensory inputs have a high probability of being integrated. To investigate possible multisensory temporal processing deficits in obesity, we investigated performance in two multisensory audiovisual temporal tasks, namely simultaneity judgment and temporal order judgment. Results showed a wider TBW in obese participants as compared to healthy-weight controls. This holds true for both the simultaneity judgment and the temporal order judgment tasks. An explanatory hypothesis would regard the effect of metabolic alterations and low-grade inflammatory state, clinically observed in obesity, on the temporal organization of brain ongoing activity, which one of the neural mechanisms enabling multisensory integration.
MDCK cells expressing constitutively active Yes-associated protein (YAP) undergo apical extrusion depending on neighboring cell status
Authors: Chiba Takanori , Ishihara Erika , Miyamura Norio , Narumi Rika , Kajita Mihoko , Fujita Yasuyuki , Suzuki Akira , Ogawa Yoshihiro , Nishina Hiroshi 
Published: June 2016

AbstractCell competition is a cell-cell interaction by which a cell compares its fitness to that of neighboring cells. The cell with the relatively lower fitness level is the “loser” and actively eliminated, while the cell with the relatively higher fitness level is the “winner” and survives. Recent studies have shown that cells with high Yes-associated protein (YAP) activity win cell competitions but the mechanism is unknown. Here, we report the unexpected finding that cells overexpressing constitutively active YAP undergo apical extrusion and are losers, rather than winners, in competitions with normal mammalian epithelial cells. Inhibitors of metabolism-related proteins such as phosphoinositide-3-kinase (PI3K), mammalian target of rapamycin (mTOR), or p70S6 kinase (p70S6K) suppressed this apical extrusion, as did knockdown of vimentin or filamin in neighboring cells. Interestingly, YAP-overexpressing cells switched from losers to winners when co-cultured with cells expressing K-Ras (G12V) or v-Src. Thus, the role of YAP in deciding cell competitions depends on metabolic factors and the status of neighboring cells.
Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway
Authors: Tang Haiping , Li Jin , Liu Xiaohui , Wang Guihuai , Luo Minkui , Deng Haiteng 
Published: June 2016

AbstractGlioblastoma is a fatal and incurable cancer with the hyper-activated mTOR pathway. HSP60, a major chaperone for maintenance of mitochondrial proteostasis, is highly expressed in glioblastoma patients. To understand the effects of HSP60 on glioblastoma tumorigenesis and progression, we characterized the HSP60-knockdowned glioblastoma cells and revealed that HSP60 silencing markedly suppressed cell proliferation and promoted cell to undergo the epithelial-mesenchymal transition (EMT). Proteomic analysis showed that ribosomal proteins were significantly downregulated whereas EMT-associated proteins were up-regulated in HSP60-knockdowned U87 cells as confirmed by a distinct enrichment pattern in newly synthesized proteins with azido-homoalanine labeling. Biochemical analysis revealed that HSP60 knockdown increased reactive oxygen species (ROS) production that led to AMPK activation, similarly to the complex I inhibitor rotenone-induced AMPK activation. Activated AMPK suppressed mTORC1 mediated S6K and 4EBP1 phosphorylation to decrease protein translation, which slowed down cell growth and proliferation. On the other hand, high levels of ROS in HSP60 knockdowned or rotenone-treated U87 cells contributed to EMT. These results indicate that HSP60 silencing deactivates the mTOR pathway to suppress glioblastoma progression, suggesting that HSP60 is a potential therapeutic target for glioblastoma treatment.
Mechanochemical conversion of brominated POPs into useful oxybromides: a greener approach
Authors: Cagnetta Giovanni , Liu Han , Zhang Kunlun , Huang Jun , Wang Bin , Deng Shubo , Wang Yujue , Yu Gang 
Published: June 2016

AbstractBrominated organic pollutants are considered of great concern for their adverse effect on human health and the environment, so an increasing number of such compounds are being classified as persistent organic pollutants (POPs). Mechanochemical destruction is a promising technology for POPs safe disposal because it can achieve their complete carbonization by solvent-free high energy ball milling at room temperature. However, a large amount of co-milling reagent usually is necessary, so a considerable volume of residue is produced. In the present study a different approach to POPs mechanochemical destruction is proposed. Employing stoichiometric quantities of Bi2O3 or La2O3 as co-milling reagent, brominated POPs are selectively and completely converted into their corresponding oxybromides (i.e. BiOBr and LaOBr), which possess very peculiar properties and can be used for some actual and many more potential applications. In this way, bromine is beneficially reused in the final product, while POPs carbon skeleton is safely destroyed to amorphous carbon. Moreover, mechanochemical destruction is employed in a greener and more sustainable manner.
Protection against doxorubicin-induced myocardial dysfunction in mice by cardiac-specific expression of carboxyl terminus of hsp70-interacting protein
Authors: Wang Lei , Zhang Tian-Peng , Zhang Yuan , Bi Hai-Lian , Guan Xu-Min , Wang Hong-Xia , Wang Xia , Du Jie , Xia Yun-Long , Li Hui-Hua 
Published: June 2016

AbstractCarboxyl terminus of Hsp70-interacting protein (CHIP) is a critical ubiquitin ligase/cochaperone to reduce cardiac oxidative stress, inflammation, cardiomyocyte apoptosis and autophage etc. However, it is unclear whether overexpression of CHIP in the heart would exert protective effects against DOX-induced cardiomyopathy. Cardiac-specific CHIP transgenic (CHIP-TG) mice and the wild-type (WT) littermates were treated with DOX or saline. DOX-induced cardiac atrophy, dysfunction, inflammation, oxidative stress and cardiomyocyte apoptosis were significantly attenuated in CHIP-TG mice. CHIP-TG mice also showed higher survival rate than that of WT mice (40% versus 10%) after 10-day administration of DOX. In contrast, knockdown of CHIP by siRNA in vitro further enhanced DOX-induced cardiotoxic effects. Global gene microarray assay revealed that after DOX-treatment, differentially expressed genes between WT and CHIP-TG mice were mainly involved in apoptosis, atrophy, immune/inflammation and oxidative stress. Mechanistically, CHIP directly promotes ubiquitin-mediated degradation of p53 and SHP-1, which results in activation of ERK1/2 and STAT3 pathways thereby ameliorating DOX-induced cardiac toxicity.
Systematic protein interactome analysis of glycosaminoglycans revealed YcbS as a novel bacterial virulence factor
Authors: Hsiao Felix Shih-Hsiang , Sutandy FX Reymond , Syu Guan-Da , Chen Yi-Wen , Lin Jun-Mu , Chen Chien-Sheng 
Published: June 2016

AbstractMicrobial pathogens have evolved several strategies for interacting with host cell components, such as glycosaminoglycans (GAGs). Some microbial proteins involved in host–GAG binding have been described; however, a systematic study on microbial proteome–mammalian GAG interactions has not been conducted. Here, we usedEscherichia coliproteome chips to probe four typical mammalian GAGs, heparin, heparan sulphate (HS), chondroitin sulphate B (CSB) and chondroitin sulphate C (CSC) and identified 185 heparin-, 62 HS-, 98 CSB- and 101 CSC-interacting proteins. Bioinformatics analyses revealed the unique functions of heparin- and HS-specific interacting proteins in glycine, serine and threonine metabolism. Among all the GAG-interacting proteins, three were outer membrane proteins (MbhA, YcbS and YmgH). Invasion assays confirmed that mutantE. colilackingycbScould not invade the epithelial cells. Introducing plasmid carryingycbScomplemented the invading defects atycbSlackingE. colimutant, that can be further improved by overexpressingycbS. Preblocking epithelial cells with YcbS reduced the percentage ofE. coliinvasions. Moreover, we observed that whole components of theycboperon were crucial for invasion. The displacement assay revealed that YcbS binds to the laminin-binding site of heparin and might affect the host extracellular matrix structure by displacing heparin from laminin.
Roots of forbs sense climate fluctuations in the semi-arid Loess Plateau: Herb-chronology based analysis
Authors: Shi Songlin , Li Zongshan , Wang Hao , von Arx Georg , Lü Yihe , Wu Xing , Wang Xiaochun , Liu Guohua , Fu Bojie 
Published: June 2016

AbstractGrowth of herbaceous plants responds sensitively and rapidly to climate variability. Yet, little is known regarding how climate warming influences the growth of herbaceous plants, particularly in semi-arid sites. This contrasts with widely reported tree growth decline and even mortality in response to severe water deficits due to climate warming around the world. Here, we use the relatively novel approach of herb-chronology to analyze the correlation between climatic factors and annual ring width in the root xylem of two perennial forb species (Medicago sativa, Potentilla chinensis) in the Loess Plateau of China. We show that warming-induced water deficit has a significant negative effect on the growth of herbaceous plants in the Loess Plateau. Our results indicate that the growth of forbs responds rapidly and sensitively to drought variability, implying that water availability plays a dominant role in regulating the growth of herbaceous plants in semi-arid areas. If warming and drying in the Loess Plateau continue in the future, further affects the growth of herbaceous plants, potentially driving regional changes in the relationship between herbaceous vegetation and climate.
A new autoinhibited kinase conformation reveals a salt-bridge switch in kinase activation
Authors: Wei Qiang , Yang Shaoyuan , Li Dan , Zhang Xiaoying , Zheng Jimin , Jia Zongchao 
Published: June 2016

AbstractIn the structure of autoinhibited EphA2 tyrosine kinase reported herein, we have captured the entire activation segment, revealing a previously unknown role of the conserved Arg762 in kinase autoinhibition by interacting with the essential Mg2+-chelating Asp757. While it is well known that this Arg residue is involved in an electrostatic interaction with the phospho-residue of the activation loop to stabilize the active conformation, our structure determination revealed a new role for the Arg, acting as a switch between the autoinhibited and activated conformations. Mutation of Arg762 to Ala in EphA2 sensitized Mg2+ response, resulting in enhanced kinase catalytic activity and Mg2+ cooperativity. Furthermore, mutation of the corresponding Arg/Lys to Ala in PKA and p38MAPK also exhibited similar behavior. This new salt bridge-mediated switch may thus be an important mechanism of activation on a broader scope for kinases which utilize autophosphorylation.