bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR-enabled point-of-care genotyping for APOL1 genetic risk assessment
Authors: Greensmith Robert , Lape Isadora T , Riella Cristian V , Schubert Alexander J , Metzger Jakob J , Dighe Anand S , Tan Xiao , Hemmer Bernhard , Rau Josefine , Wendlinger Sarah , Diederich Nora , Schütz Anja , Riella Leonardo V , Kaminski Michael M 
Published: September 2024

AbstractDetecting genetic variants enables risk factor identification, disease screening, and initiation of preventative therapeutics. However, current methods, relying on hybridization or sequencing, are unsuitable for point-of-care settings. In contrast, CRISPR-based-diagnostics offer high sensitivity and specificity for point-of-care applications. While these methods have predominantly been used for pathogen sensing, their utilization for genotyping is limited. Here, we report a multiplexed CRISPR-based genotyping assay using LwaCas13a, PsmCas13b, and LbaCas12a, enabling the simultaneous detection of six genotypes. We applied this assay to identify genetic variants in the APOL1 gene prevalent among African Americans, which are associated with an 8–30-fold increase in the risk of developing kidney disease. Machine learning facilitated robust analysis across a multicenter clinical cohort of more than 100 patients, accurately identifying their genotypes. In addition, we optimized the readout using a multi-analyte lateral-flow assay demonstrating the ability for simplified genotype determination of clinical samples. Our CRISPR-based genotyping assay enables cost-effective point-of-care genetic variant detection due to its simplicity, versatility, and fast readout.
Cardiovascular health (“Life’s Essential 8”), risk of depression and anxiety: a prospective cohort study
Authors: Liu Shuzhen , Hu Xiangju , Jiang Meijie , Huang Ninghao , Liang Hailun , Zheng Ruimao , Wang Shuyan , Qin Jian , Zhang Zhiyong , Huang Tao , Gao Xu 
Published: September 2024

AbstractThere is a growing interest in the linkage of cardiovascular health (CVH) with depression/anxiety but the evidence of “Life’s Essential 8” CVH score is scarce. We evaluated the associations of CVH score with risk of incident depression/anxiety among ~0.4 million participants. During follow-up, 17,554 incident events with symptoms of either disorder were recorded. Per 100-point decrease in CVH score was associated with an increased risk of incident either disorder (Hazard ratio [HR] = 1.133, 95% confidence interval [CI]:1.114–1.153), depression (HR = 1.205, 95% CI:1.180–1.231), and anxiety (HR = 1.042, 95% CI:1.017–1.069). Per 100-point decrease in health assessments or health behaviors was associated with an increased risk of incident either disorder (HRhealth assessments = 1.085, 95% CI: 1.058–1.113, HRhealth behaviors = 1.217, 95% CI: 1.186–1.250). Poor CVH is a risk factor for the incident late-life depression/anxiety symptoms of middle-aged and older adults, and healthy behaviors could be targeted for the risk assessment and intervention of depression/anxiety.
CaMKK2: bridging the gap between Ca2+ signaling and energy-sensing
Authors: McAloon Luke M. , Muller Abbey G. , Nay Kevin , Lu Eudora L. , Smeuninx Benoit , Means Anthony R. , Febbraio Mark A. , Scott John W. 
Published: September 2024

Abstract
Calcium (Ca2+) ions are ubiquitous and indispensable signaling messengers that regulate virtually every cell function. The unique ability of Ca2+ to regulate so many different processes yet cause stimulus specific changes in cell function requires sensing and decoding of Ca2+ signals. Ca2+-sensing proteins, such as calmodulin, decode Ca2+ signals by binding and modifying the function of a diverse range of effector proteins. These effectors include the Ca2+-calmodulin dependent protein kinase kinase-2 (CaMKK2) enzyme, which is the core component of a signaling cascade that plays a key role in important physiological and pathophysiological processes, including brain function and cancer. In addition to its role as a Ca2+ signal decoder, CaMKK2 also serves as an important junction point that connects Ca2+ signaling with energy metabolism. By activating the metabolic regulator AMP-activated protein kinase (AMPK), CaMKK2 integrates Ca2+ signals with cellular energy status, enabling the synchronization of cellular activities regulated by Ca2+ with energy availability. Here, we review the structure, regulation, and function of CaMKK2 and discuss its potential as a treatment target for neurological disorders, metabolic disease, and cancer.
Multi‐layer graphene nanosheets bridging binary aluminium oxide for the synergistic enhancement of thermal conductivity and electrical insulation of silicone resin composite
Authors: Shen Yutan , Li Shikun , Qin Bing , Zhang Chong , Li Wenpeng , Wang Bo , Zhu Zhanwei , Liu Chang 
Published: September 2024

AbstractThe flexible polymer composites usually require high intrinsic thermal conductivity fillers for the applications in thermal management, such as the well‐known graphene, which can, in turn, compromise their electrical insulation properties. Here, the authors developed the silicone resin (SR) composites filled with multi‐layer graphene nanosheets (MGN) and binary alumina (Al2O3), and the microstructure of composites exhibits the dominant skeleton of large‐sized Al2O3 filler and branching of small‐sized Al2O3 fillers features by the size matching effect of optimal binary Al2O3. The introduction of supplementary MGN fillers further increases the face‐to‐face contact probability and bridges the isolated binary Al2O3, which contributes to the high thermal conductivity of 3.0 W·m−1·K−1 under these synergistic effects. The dense and connected ‘Al2O3‐MGN‐Al2O3’ thermal conductive pathway is successfully built, as supported by the numerical simulation and Agari model fitting. Meanwhile, the electrical conductivity caused by MGN can be blocked by the surrounding insulation Al2O3 filler network, exhibiting a high volume resistivity of 1.7 × 1015 Ω · cm. Moreover, the Al2O3/MGN/SR composite films effectively transfer the heat and diminish the hot‐spot temperature by 53.5°C in cooling light emitting diode chip module application.
The effect of the environment-dependent stellar initial mass function on the photometric properties of star-forming galaxies
Authors: Haslbauer Moritz , Yan Zhiqiang , Jerabkova Tereza , Gjergo Eda , Kroupa Pavel , Hasani Zonoozi Akram 
Published: September 2024

Context. Observational estimates of galaxy properties, including mass and star formation rates (SFRs), rely on the inherent galaxy-wide initial mass function (gwIMF), which systematically varies with the global SFR and metallicity, as proposed by the integrated-galactic IMF (IGIMF) theory and supported by empirical evidence.
Aims. We aim to evaluate the influence of the variable gwIMF on various galaxy properties, encompassing the Ks-, K3.6-, and V-band stellar mass-to-light ratio, SFR–luminosity relation, gas depletion timescale, and stellar mass buildup timescale of local star-forming galaxies.
Methods. We incorporate PARSEC and COLIBRI stellar isochrones into the GalIMF code, a galaxy chemical evolution (GCE) model featuring real-time updates of environment-dependent gwIMFs. This newly developed photometric GalIMF (photGalIMF) code allows the calculation of photometric properties for galaxies with diverse stellar populations. Subsequently, we analyze observed luminosities and metallicities of local star-forming galaxies to deduce their stellar masses assuming empirically-motivated SFHs of Local Cosmological Volume galaxies. We also compute SFR–Hα luminosity relations for varying stellar metallicities using a separate stellar population synthesis code based on PÉGASE.
Results. Comparing the IGIMF theory to the canonical universal IMF, our analysis reveals that estimates of the stellar masses and SFRs for local star-forming galaxies differ by factors of approximately 2 and 10, respectively. This disparity yields a well-defined galaxy main sequence extending to dwarf galaxies. The computed gas-depletion timescale increases with gas mass, implying lower star formation efficiencies in more massive galaxies, possibly due to stronger feedback regulation, aligning with theoretical expectations. Additionally, the characteristic stellar mass buildup timescale increases with stellar mass, indicating that massive disk galaxies initiate star formation earlier than their low-mass counterparts.
Conclusions. The photGalIMF code enables self-consistent computations of galactic photometry with GCE modelling adopting an environment-dependent gwIMF. Utilizing K-band and Hα luminosities of galaxies, the outcomes include galaxy mass, SFR, and fitting functions for the SFR correction factor.
Plasma wave survey from Parker Solar Probe observations during Venus gravity assists
Authors: George H. , Malaspina D. M. , Lee-Bellows D. , Gasque L. C. , Goodrich K. , Ma Y. , Curry S. 
Published: September 2024

Context. Parker Solar Probe (PSP) performs Venus gravity assists (VGAs) in order to lower its perihelion. PSP takes high-cadence electric and magnetic field observations during these VGAs, providing the opportunity to study plasma waves in Venus’s induced magnetosphere.
Aims. We summarize the plasma environment during these VGAs, including the regions of near-Venus space that PSP traversed and the key boundary crossings. We comprehensively identify Langmuir, ion acoustic, whistler-mode, and ion cyclotron waves during these VGAs and map the location of these waves throughout near-Venus space.
Methods. This study analyzes different data products from the PSP FIELDS instrument suite from throughout the first five VGAs.
Results. We compare the FIELDS instrumentation capabilities to the capabilities of the plasma wave instruments on board the Pioneer Venus Orbiter (PVO) and the Venus Express (VEX). We find that the PVO electric field instrument was well suited to observe Langmuir waves, especially near the bow shock and in the foreshock. However, evaluation of the other plasma waves detected by PSP FIELDS reveals that PVO and VEX would have often been unable to observe key features of these waves modes, including maximum power, bandwidth, and propagation direction. These wave characteristics provide critical information on the wave generation mechanisms and wave-particle interactions, so provide fundamental information on the nature of Venus’s induced magnetosphere.
Conclusions. These results highlight the advances in plasma wave instrumentation capabilities that have been made in the decades since the PVO and VEX eras, and illustrate the value of a plasma wave instrument on a new Venus mission.
Einfluss sportlichen Trainings auf die Fatigue bei Patient*innen mit
Multipler Sklerose
Authors: Meyer Victoria Charlotte , Schmidt Jennifer 
Published: September 2024

ZusammenfassungDie Fatigue ist eines der häufigsten Symptome der Multiplen Sklerose (MS).
Gekennzeichnet durch unverhältnismäßige Erschöpfung nach physischer oder
kognitiver Belastung führt sie zu massiven Beeinträchtigungen in sämtlichen
Lebensbereichen. Die Therapie der MS-assoziierten Fatigue stützt sich vor allem
auf nicht-medikamentöse Maßnahmen. Sportliches Training kann sich positiv auf
verschiedene Symptome der MS auswirken. Hinsichtlich der Fatigue wird empfohlen,
Betroffene über die Effekte körperlichen Trainings aufzuklären. Bislang bestehen
allerdings nur wenige Erkenntnisse über eine optimale Trainingsgestaltung.Ziel dieser Arbeit ist es, anhand aktueller Forschungserkenntnisse den Einfluss
sportlichen Trainings auf die Fatigue bei Patient*innen mit MS zu analysieren
und anhand dessen mögliche Trainingsempfehlungen abzuleiten. Dafür wurden die
Fachdatenbanken PubMed, Cochrane Library, PEDro und BISp Surf systematisch nach
randomisierten kontrollierten Studien (RCTs), welche die Auswirkungen
sportlichen Trainings auf die Fatigue bei Patient*innen mit MS analysierten,
durchsucht. Eine Bewertung der methodischen Qualität der Arbeiten erfolgte
anhand der PEDro-Skala. Die Effektgröße wurde mittels Cohen's d kalkuliert.
Neun RCTs (n=564) wurden eingeschlossen. Die durchschnittliche methodische
Qualität lag mit 6,9 Punkten auf der PEDro-Skala bei „gut“.Die Ergebnisse der Studien zeigten, dass sportliches Training die Fatigue bei
Patient*innen mit MS reduzieren kann. Das Ausmaß der Linderung fällt je nach Art
der Belastung allerdings unterschiedlich aus. Krafttrainingsformen zeigten die
höchste Effektivität. Um die langfristige Aufrechterhaltung der positiven
Effekte zu gewährleisten, ist ein kontinuierliches Training erforderlich.Die Aussagekraft der Ergebnisse ist durch die hohe Heterogenität der Arbeiten
sowie die begrenzte Vergleichbarkeit der verwendeten Assessments limitiert.
Aufgrund unzureichender Daten über die optimale Gestaltung von
Belastungsparametern und individuelle Einflussfaktoren ist aktuell eine
spezifizierte Trainingsempfehlung nur eingeschränkt möglich und bedarf weiterer
Forschung.
Exploring the Impact of GitHub Copilot on Health Informatics Education
Authors: Avramovic Sanja , Avramovic Ivan , Wojtusiak Janusz 
Published: September 2024

Abstract
Background The use of artificial intelligence-driven code completion tools, particularly the integration of GitHub Copilot with Visual Studio, has potential implications for Health Informatics education, particularly for students learning SQL and Python.
Objectives This study aims to evaluate the effectiveness of these tools in solving or assisting with the solution of problems found in Health Informatics coursework, ranging from simple to complex.
Methods The study assesses the performance of GitHub Copilot in generating code to solve programming problems normally given to students in introductory Health Informatics programming courses. Problem statements are provided to the tool; the response is assessed on correctness. The focus is on the impact of detailed explanations on the tool's effectiveness.
Results Findings reveal that GitHub Copilot can generate correct code for straightforward problems. The correctness and effectiveness of solutions decrease with problem complexity, and the tool struggles with the most challenging problems, although performance on complex problems improves with more detailed explanations.
Conclusion The study not only underscores the relevance of these tools to programming in Health Informatics education but also highlights the need for critical evaluation by students. It concludes with a call for educators to adapt swiftly to this rapidly evolving technology.
Anisotropic optical and magneto-optical properties of antiferromagnetic Weyl semimetal Mn3Sn epitaxial thin films
Authors: Gao Dong , Yang Ting , Tang Fu , Su Jiejun , Yang Weihao , Deng Dengfu , Xie Yunfei , Qin Jun , Liang Xiao , Bi Lei 
Published: September 2024

Antiferromagnetic Weyl semimetal Mn3Sn exhibiting strong magneto-optical Kerr effect (MOKE) due to non-zero Berry curvature is attractive for spintronic and photonic device applications. Despite many reports on the anisotropic anomalous Hall effect (AHE), so far, there have been few studies on its anisotropic optical properties. In this work, we experimentally characterized the anisotropic optical and magneto-optical (MO) properties of Mn3Sn(20)/MgO(110) and Mn3Sn(0001)/Al2O3(0001) epitaxial films using ellipsometry in the wavelength range from 300 to 1690 nm. By measuring the Mueller matrix of magnetized Mn3Sn, the anisotropic permittivity tensor is determined using the 4 × 4 transfer matrix method. Temperature dependent MOKE measurement confirmed the origin of the anisotropic MO effect to the non-zero Berry curvature of the chiral magnetic phase. The measured permittivity also agrees well with first-principles calculations. The anisotropic optical and MO properties determined in this work can be useful for Mn3Sn based spintronic device characterization and photonic device development.
Shedding light on predator detections: evaluating the impact of camera-trap flash type for feral cat monitoring through in-field observations
Authors: Paton Alexandra J. , Buettel Jessie C. , Brook Barry W. 
Published: September 2024

White flash camera-traps are avoided in predator monitoring as they are assumed to lower redetection, despite infrared cameras producing lower-quality night images thus limiting the scope for individual identification and, consequently, the accuracy of density estimates. We sought to determine whether flash type impacts the behaviour of cats (Felis catus). We identified different behavioural responses exhibited by photographed cats, and quantitatively assessed relative activity, redetection rates, and the activity of specific individuals using 11,389 images of feral cats from 62 white flash and 62 infrared camera-traps across Tasmania. We found no difference in the relative activity of cats between flash types (odds ratio of 0.90, [null expectation = 1], CI = 0.55, 1.47), and there was no evidence of a reduction in redetection rate of feral cats following initial detection (odds ratio = 0.83, CI = 0.47, 1.46). The activity of individuals was similar between white flash (average = 0.026, CI = 0.021, 0.032), and infrared cameras (average = 0.028, CI = 0.022, 0.035). White flash cameras appear suitable for monitoring feral cats without resulting in a negative bias, highlighting the need for researchers to critically examine assumptions regarding best methodology.
Discourse of self-legitimation
Authors: Brusenbauch Meislová Monika 
Published: September 2024

Abstract
The article proposes an original conceptual framework that captures the discursive logic of self-legitimation and
applies it to the empirical case of European Union soft law and the process of Brexit. Adopting the general orientation of
Discourse Historical Approach in Critical Discourse Analysis and working with a dataset of all European Parliament (EP)
resolutions on Brexit in the 2016–2023 period, the article investigates how, and interprets the particular ways in which, the EP
legitimised itself as an actor vis-à-vis Brexit, through different linguistic and discursive devices in its resolutions. The
analysis illustrates that the EP’s use of soft law for self-legitimation purposes is an intriguing case of how legal instruments
can be leveraged in multifaceted ways beyond their traditional function for broader communicative and political purposes. As such,
the article extends the traditional understanding of the purposes of EP resolutions by explicitly acknowledging them as powerful
discursive resources for self-legitimation.
Subverting EU legal concepts
Authors: Luining Michiel , Van Hout Tom 
Published: September 2024

Abstract
This paper analyzes how Hungarian constitutional court rulings subvert EU legal discourse. Drawing on legal
studies and discourse studies, we examine how the Fidesz-KDNP regime leverages the Hungarian Constitutional Court to curtail the
rights of asylum seekers and migrants within an evolving EU legal framework of identity recognition. We show how an illiberal
agenda was enacted in a populist constitutional imaginary that, instead of outright rejecting established legal concepts, inflects
these concepts with subversive new meanings. Our analysis reveals how the legal principles of the rule of law, human dignity,
sincere cooperation and EU constitutional identity are recontextualized to legitimize a politically exclusionary agenda and
redefine the moral boundaries of political inclusion and exclusion within the EU.
Sensescapes in multilingual environments
Authors: Prada Josh 
Published: September 2024

Abstract
Sensescapes refer to the complex multimodal and semiotic assemblages that confer meaning to space as experienced
by individuals and groups. The study of sensescapes seeks to understand the nature of these assemblages, how they shape meaning,
and with them, individual and collective experiences. This article unpacks sensescapes as an expansive lens to meaning in space,
underscoring its potential to investigate the nexus between language and culture. To that end, it integrates existing proposals in
linguistic landscapes and translanguaging, and connects them to a working definition of sensescapes drawn from various social
sciences and humanities fields. To illustrate the study of sensescapes and their relevance to applied linguistics, the article
reports on a case study conducted in the Al-Kissaria district of Nador, Morocco through participatory ethnography. The study
provides a phenomenological account of the experiences of two multilingual sisters from neighboring Melilla, Spain, as they
navigate a market area. The data illustrate how the sisters’ sensory interactions with the environment interplay with their
linguistic and broader semiotic repertoires, activating their previous experiences and shaping their conceptualizations and
negotiation of language and culture in this space.
Phase transition investigation of ferroelectric and dielectric properties of (NH
2
CH
2
COOH)3

H

2

SO

4

crystal using green function method and two sublattice PLCM model Hamiltonian
Authors: Iqbal Khan Muzaffar , Upadhyay Riya , Chandra Upadhyay Trilok , Asghar Shah Ali 
Published: September 2024

Abstract
The Greens function technique and extended two sublattice pseudospin lattice coupled-mode (PLCM) model Hamiltonian have been applied to study the phase transition mechanism in

(

NH

2

CH

2

COOH
)

3

H

2

SO

4

compound. The two sublattice PLCM model Hamiltonian is modified by considering anharmonic phonon interactions, extra spin-lattice interactions, and an external applied electric field term. By using the double-time thermal-dependent Green function technique, the theoretical equations for the total shift, total width, normal mode frequency, transition temperature, relative permittivity, dielectric tangent loss, acoustic attenuation and spontaneous polarization are derived. By fitting the distinct model parameters in the theoretical equations, we calculated the temperature dependence of normal mode frequency, relative permittivity, tangent loss, acoustic attenuation and spontaneous polarization. The numerically obtained results are hold well and correlated with experimental data.
Enhanced capability of model fitting to nuclear level density and heat capacity: testing on 93−98Mo nuclei
Authors: Ngoc Anh Nguyen , Nhut Huan Phan , Quang Hung Nguyen , Xuan Hai Nguyen 
Published: September 2024

Abstract
We propose an improved fitting approach that improves reliability in studying the nuclear level density (NLD) and thermodynamic quantities. The proposed method, which relies on the fact that experimental fluctuations or outliers, if they exist, should not be involved in the fitting process, is validated with a set of data artificially generated with anomalous data points being intentionally inserted. In order to showcase the advantages of the proposed technique, we have applied it to re-investigate the back-shifted Fermi gas (BSFG) level density parameters and thermodynamic quantities, particularly the heat capacities, of 93−98Mo isotopes. We have found that the range of values for the level density parameter of 93Mo (approximately from 8.5 to 9.0 MeV−1) is notably smaller than that obtained for the other isotopes of Mo (approximately from 10.5 to 11.5 MeV−1). This observation is different from previous predictions, in which the values of level density parameter of all Mo isotopes are in the same range. This is because among the Mo isotopes under examination, 93Mo (N = 51 neutrons) has the smallest number of valence neutrons, namely only a single neutron away from the closed N = 50 shell. In addition, thanks to the proposed method, we have discussed the effects of data fluctuations on the BSFG NLDs and thermodynamic quantities of 93−98Mo isotopes, from which our recommendation for future works is announced. On top of that, we should notice that the proposed approach can be further applied to any work involving the fitting of a phenomenological model to empirical data.