bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pcsk5 is required in the early cranio-cardiac mesoderm for heart development
Authors: Szumska Dorota , Cioroch Milena , Keeling Angela , Prat Annik , Seidah Nabil G. , Bhattacharya Shoumo 
Published: April 2017

Abstract
Background
Loss of proprotein convertase subtilisin/kexin type 5 (Pcsk5) results in multiple developmental anomalies including cardiac malformations, caudal regression, pre-sacral mass, renal agenesis, anteroposterior patterning defects, and tracheo-oesophageal and anorectal malformations, and is a model for VACTERL/caudal regression/Currarino syndromes (VACTERL association - Vertebral anomalies, Anal atresia, Cardiac defects, Tracheoesophageal fistula and/or Esophageal atresia, Renal & Radial anomalies and Limb defects).

Results
Using magnetic resonance imaging (MRI), we examined heart development in mouse embryos with zygotic and cardiac specific deletion of Pcsk5. We show that conditional deletion of Pcsk5 in all epiblastic lineages recapitulates all developmental malformations except for tracheo-esophageal malformations. Using a conditional deletion strategy, we find that there is an essential and specific requirement for Pcsk5 in the cranio-cardiac mesoderm for cardiogenesis, but not for conotruncal septation or any other aspect of embryonic development. Surprisingly, deletion of Pcsk5 in cardiogenic or pharyngeal mesodermal progenitors that form later from the cranio-cardiac mesoderm does not affect heart development. Neither is Pcsk5 essential in the neural crest, which drives conotruncal septation.

Conclusions
Our results suggest that Pcsk5 may have an essential and early role in the cranio-cardiac mesoderm for heart development. Alternatively, it is possible that Pcsk5 may still play a critical role in Nkx2.5-expressing cardiac progenitors, with persistence of mRNA or protein accounting for the lack of effect of deletion on heart development.
Medium- and long-term consequences of pollution on labor supply: evidence from Indonesia
Authors: Kim Younoh , Manley James , Radoias Vlad 
Published: April 2017

Abstract
We use a natural experiment in Indonesia to study the medium- and long-term effects of air pollution on labor supply. We find that exposure to air pollution reduces hours worked and while the medium-term effects are larger in magnitude, some effects do persist in the long term. More interestingly, we are able to provide some insight regarding the underlying channels that contribute to the reduced labor supply. Own health seems to be the only responsible channel in the long term, while in the medium term, a different channel based on dependent caregiving is the most important.

JEL Classification: J22, Q53
What is nature conservation for the Portuguese public? Understanding the commonsensical image of nature to engage the general public in conservation efforts and to improve the communication between science and society
Authors: Vieira da Silva Carina , Costa José Lino , Santos José Lima , Madureira Lívia 
Published: April 2017

Abstract
The protection of nature and biodiversity has been gaining importance in public opinion. Nevertheless, the implementation of conservation policies has experienced some public resistance, along with low levels of public involvement. This lack of public interest in and support to conservation may be related to citizens’ images of nature.
In this article, we examine the commonsensical image of nature and its conservation according to two dimensions: cognitive beliefs and normative ethics. This image of nature was examined through a series of focus-group discussions with members of the general public in the Lisbon Metropolitan Area – Portugal and reflects the commonsensical perceptions of what nature conservation is.
The results suggest that, although the participants were not familiar with scientific terminology, they hold rich, mental and social representations about nature and biodiversity. We present a conceptual framework that reflects participants’ overall image of nature as the basis of human life and, above all, as a source of multiple goods that ensure human beings’ survival, which characterizes the common-sense view of nature as largely anthropocentric.
Our work provides policy-makers, planners and managers with a framework that reflects lay people’s perceptions of nature and biodiversity conservation that are instrumental in identifying conservation priorities, appropriate goals and suitable measures while ensuring communication between science and society.
State, science and empire in the Portuguese Atlantic (1770s–1820s)
Authors: Carolino Luís Miguel 
Published: April 2017

Abstract
This article argues that the strengthening of an Atlantic-based economy was the driving force of scientific development in the Portuguese empire during the late eighteenth and early nineteenth centuries. As a response to the structural crisis that distressed the Portuguese empire in the second half of the eighteenth century, a network of military institutions of higher technical and scientific education was established, first in Lisbon and later in Rio de Janeiro. State initiative was thus crucial in the modernizing process of the Luso-Brazilian empire. This produced profound consequences in the shaping of its scientific and technical framework. In particular, it resulted in the militarization of ‘Portuguese’ science, in the promotion of an applied science and in the social impact of a conception of the concept of the scientist as a military and practical man.
In vitro study: binding of 99mTc-DPD to synthetic amyloid fibrils
Authors: Buroni Federica E , Persico Marco G , Lodola Lorenzo , Concardi Monica , Aprile Carlo 
Published: April 2017

Abstract
This paper is an report of the investigation of the in vitro binding of 99mTc-DPD for synthetic amyloid fibrils used for the diagnosis of cardiac amyloidosis (CA), as compared with the use of 99mTc-HMDP and 99mTc-PPI. It also includes an inquiry into the role played by Ca2+ ions and serum proteins on binding to amyloid like materials, as well as the saturability and specificity of DPD for fibrils versus amorphous precipitates (AP).
In the work, synthetic insulin fibrils (SIF) and AP were characterized by Congo red staining and TEM imaging. An equal amount of three radiopharmaceuticals were then added to fibrils in Ca2+ (0-4.2 mmol/L) or human serum (HS) adjoined samples and radiopharmaceutical uptake was assessed. To test the saturability of amyloid binding sites, a displacement assays with cold DPD was performed, while adding 50-1500 nmol of 99mTc-DPD to SIF or AP, saturation binding tests were subsequently carried out for evaluating its specificity for amyloid.
Herein, synthetic fibrils and AP showed conformational differences at TEM and polarized microscopy analysis. In our study, 99mTc-DPD fibrils uptake was seen to be the highest and increased with calcium ions concentration. What is more, serum proteins reduced the bound fraction to the amyloid deposits of about 15%, and the Kd values of 90 nM and 114 nM relative to SIF and AP, respectively, did not significantly differ.
We saw that 99mTc-DPD is the best seeker for amyloid fibrils in cardiac amyloidosis, and that Ca2+ concentration positively influenced DPD fibrils binding. Furthermore, the radioactivity bound to the serum protein clear up the idea of nuclide exchanging dynamic balance between amyloid and circulating proteins. Moreover, non-labeled DPD did not exert a competition for 99mTc-DPD binding sites, and, finally, DPD cannot be defined a radiopharmaceutical specific for amyloid deposits.
Littoral cell angioma mimicking metastatic tumors
Authors: Szumilo Justyna , Ostrowska Anna , Zdunek Malgorzata , Rudzki Slawomir , Chroscicki Tomasz , Czekajska-Chehab Elzbieta , Burdan Franciszek 
Published: April 2017

Abstract
Littoral cell angioma is a rare primary, vascular tumor thought to originate from the endothelial cells lining the sinuses of the splenic red pulp (the “littoral cells”). It is a benign, usually asymptomatic lesion diagnosed incidentally. Ultrasound and tomography appearance is not characteristic and histopathological examination is required. This work provides a case-study of littoral cell angioma which was seen in a 55-year-old female who complained of non-specific upper abdominal pain. Computed tomography revealed multiple hypo-attenuated splenic lesions suggestive for metastasis. A splenectomy was performed and routine microscopic examination supported by immunohistochemistry reactions with CD68, CD34 and CD31 showed littoral cell angioma.
Antimicrobial activity of petroleum ether and methanolic extracts from fruits of Seseli devenyense Simonk. and the herb of Peucedanum luxurians Tamam.
Authors: Widelski Jaroslaw , Grzegorczyk Agnieszka , Malm Anna , Chinou Ioanna , Głowniak Kazimierz 
Published: April 2017

Abstract
Plants of the Apiaceae family usually contain coumarins. These are used worldwide in traditional medicine, as well as in modern therapeutics. The aim of our study was to determine the antimicrobial activity of four extracts (methanolic and petroleum ether extracts) obtained from two Apiaceae species: Seseli devenyense (fruits) and Peucedanum luxurians (herb).
The activity of the investigated extracts was tested against 7 strains of Gram+ bacteria, and 7 strains of Gram-, as well as three of yeast. The results of this show that the best activity of such extracts (specifically, by way of petroleum ether) was seen as being against Staphylococcus aureus strains.
The impact of non-pharmacological treatments for diseases of the locomotor system on the prescribed drug use and lifestyles of the patients in the sanatoria in Busko-Zdroj
Authors: Korczak Milena , Owczarek Jacek 
Published: April 2017

Abstract
The article is the result of research on the impact of non-pharmacological therapies for diseases of the locomotor system on the prescribed drug use and lifestyles of the patients of the sanatoria in Busko-Zdroj. The reported research uses primary and secondary measures. The former includes the assessment of the impact of non-pharmacological sanatorium treatments for locomotor system diseases on the use of prescribed drug regimes, while the latter is aimed at assessing the patients’ quality of life. The research was conducted on adult patients of both genders in the sanatoria in Busko-Zdroj. The subjects were patients suffering from disorders of the musculoskeletal system such as rheumatoid arthritis, ankylosing spondylitis, osteoarthritis, osteoporosis and discopathies. The research included two visits, the first at the start of the research and the second at the end of the research. The patients were examined for a period of three consecutive weeks. The study involved 170 patients, 50% of them were women and 50% men.
A decline in the use of painkillers and anti-inflammatory drugs makes a very interesting finding. After the first week, as many as 38% of the examined patients limited the use of painkillers and/or anti-inflammatory drugs. After the second week, 62% of the patients reduced the use of the drugs, and after the third week of treatment, up to 90% of the patients did so. The improvement of patients’ lives is noticeable in the psychological, physical and motor fields.
Effect of target power on the physical properties of Ti thin films prepared by DC magnetron sputtering with supported discharge
Authors: Kavitha A. , Kannan R. , Rajashabala S. 
Published: April 2017

AbstractThe present paper describes the effect of target power on the properties of Ti thin films prepared by DC magnetron sputtering with (triode mode) and without (diode mode) supported discharge. The traditional diode magnetron sputtering with an addition of a hot filament has been used to sustain the discharge at a lower pressure. The effect of target power (60, 80, 100 and 120 W) on the physical properties of Ti thin films has been studied in diode and triode modes. XRD studies showed that the Ti thin films prepared at a target power up to 100 W in diode mode were amorphous in nature. The Ti thin films exhibited crystalline structure at much lower target power of 80 W with a preferred orientation along (0 0 2) plane. The grain size of Ti thin films prepared in triode mode increased from 64 nm to 80 nm, whereas in diode mode, the grain size increased from 2 nm to 5 nm. EDAX analysis confirmed that the incorporation of reactive gases was lower in triode mode compared to diode mode. The electrical resistivity of Ti thin films deposited in diode mode was found to be 85 µΩ⋅cm (target power 120 W). The electrical resistivity of Ti thin films in triode mode was found to be deceased to 15.2 µΩ⋅cm (target power 120 W).
Linking environmental observations and solid solution thermodynamic modeling: the case of Ba- and Sr-rich micropearls in Lake Geneva
Authors: Thien Bruno , Martignier Agathe , Jaquet Jean-Michel , Filella Montserrat 
Published: April 2017

Abstract
Intracellular inclusions of amorphous Ba- and Sr-rich calcium carbonates – referred to as “micropearls”– have recently been detected in Lake Geneva. These micropearls are formed under conditions of pronounced Ba and Sr undersaturation in the lake waters. Their formation can be explained by the ability of certain microorganisms to preconcentrate these trace elements in tandem with a non-equilibrium solid-solution growing mechanism.
A real negative selection algorithm with evolutionary preference for anomaly detection
Authors: Yang Tao , Chen Wen , Li Tao 
Published: April 2017

AbstractTraditional real negative selection algorithms (RNSAs) adopt the estimated coverage (c0) as the algorithm termination threshold, and generate detectors randomly. With increasing dimensions, the data samples could reside in the low-dimensional subspace, so that the traditional detectors cannot effectively distinguish these samples. Furthermore, in high-dimensional feature space,c0cannot exactly reflect the detectors set coverage rate for the nonself space, and it could lead the algorithm to be terminated unexpectedly when the number of detectors is insufficient. These shortcomings make the traditional RNSAs to perform poorly in high-dimensional feature space. Based upon “evolutionary preference” theory in immunology, this paper presents a real negative selection algorithm with evolutionary preference (RNSAP). RNSAP utilizes the “unknown nonself space”, “low-dimensional target subspace” and “known nonself feature” as the evolutionary preference to guide the generation of detectors, thus ensuring the detectors can cover the nonself space more effectively. Besides, RNSAP uses redundancy to replacec0as the termination threshold, in this way RNSAP can generate adequate detectors under a proper convergence rate. The theoretical analysis and experimental result demonstrate that, compared to the classical RNSA (V-detector), RNSAP can achieve a higher detection rate, but with less detectors and computing cost.
Investigation of a curve using Frenet frame in the lightlike cone
Authors: Kulahci Mihriban 
Published: April 2017

AbstractOften times the language of mathematics is used to formulate physical theories. For example, as in this paper, while Minkowski space or the theory of special relativity were studying, their formulation was given by means of mathematical methods. In this manuscript, we study spacelike normal curves lying entirely in the 2-dimensional and 3-dimensional lightlike cone. In particular, some related theorems and definitions are also given. The study of representations of spacelike normal curves in the lightlike cone has led to the existence of different areas of mathematics and physics.
Hora de tirar o espartilho – A problemática feminina nos contos de Lygia Fagundes Telles
Authors: Teixeiro Alva Martínez 
Published: April 2017

Este artigo considera como os problemas femininos são apresentados nos contos de Lygia Fagundes Telles, avaliando os procedimentos narrativos e as opções temáticas da autora, tais como a crueldade, a dependência, o conservadorismo, a emancipação, a solidão, a vaidade ou a decrepitude. O estudo analisa a originalidade e modernidade da prosa de Telles, salientando a cautelosa distância da sua visão da condição feminina de qualquer tendência contemporânea ou etiquetagem. Por último, o texto pretende também estudar o retrato das mulheres realizado pela autora. Tal objetivo exige um cuidadoso exame do modo como as mulheres assumem o rol de sujeitos e do grau de individuação dessas personagens femininas, que, nos contos, enfrentam diferentes conflitos em diversas situações sociais, familiares e afetivas.********************************************************************Hora de tirar o espartilho – Women’s issues in the short storiesof Lygia Fagundes TellesAbstract: This paper considers how womens’ issues are presented in Lygia Fagundes Telles’ short stories, evaluating narrative procedures and thematic choices, such as wickedness, dependence, conservatism, emancipation, loneliness, vanity or decrepitude. This study analyses the originality and modernity of Telles’ prose, noting the cautious distance of her vision of the feminine condition from any contemporary trends or labeling. Finally, the paper also aims to study the portrayal of women by Lygia Fagundes Telles. This simplies careful examination of how women occupy the role of the self, or subject, and to what degree Telles’ womens’ characters are individualized figures, facing different conflicts, in a variety of social, familial and affective situations.Keywords: short stories; Lygia Fagundes Telles; feminine condition.
MIGRACIÓN DE FUENTES SÍSMICAS A LO LARGO DEL CINTURÓN DE FUEGO DEL PACÍFICO
Authors: López Adriana , Villarreal Edwin , Álvarez César Ivan 
Published: April 2017

<p>El presente artículo está basado en el análisis de datos correspondientes a sismos generados a lo largo del Cinturón de Fuego del Pacífico en el período desde 1970 a 2014, utilizando la base de datos IRIS (Incorporated Research Institutions for Seismology). Con este análisis se determinó que existe una migración de fuentes sísmicas en esta región. Además, de los resultados obtenidos se encontró que esta migración se produce en sentido anti horario en la mayor parte de zonas, a excepción de las zonas que comprenden la Placa Australiana y la Placa del Pacífico. </p>
Garrincha sob a ótica do herói: uma análise de “Alegria do Povo” e “Estrela Solitária”
Authors: Marques José Carlos , Rosa Bruno Navarini 
Published: April 2017

O presente estudo tem por objetivo analisar as narrativas presentes no documentário Garrincha, alegria do povo (1963, direção de Joaquim Pedro de Andrade) e a biografia Estrela solitária – um brasileiro chamado garrincha (1995, Ruy Castro), estabelecendo uma comparação entre as trajetórias do futebolista Mané Garrincha retratadas nestas duas obras e a estrutura da Jornada do Herói de Joseph Campbell, um dos mais renomados estudiosos da mitologia universal. As reflexões propostas visam analisar a possibilidade de enquadramento do atleta no âmbito do mito, o que se dá pela verificação da passagem de Garrincha pelo núcleo Separação–Iniciação–Retorno do monomito, percurso padrão da aventura mitológica do herói, uma figura predestinada a superar desafios e a realizar feitos grandiosos.
A study of cutaneous adverse drug reactions in the outpatient department of Dermatology at a tertiary care center in Gujarat, India
Authors: Chavda Dinesh A. , Suthar Satish D. , Singh Shradhanand , Balat Jayesh D. , Parmar Sailesh P. , Mistry Suresh D. 
Published: April 2017

Background: The data for adverse cutaneous drug reactions (ACDRs) is limited in Gujarat. The ACDRs are one of the frequent ADRs and cause of significant morbidity and mortality in patients of all areas of healthcare today. They are responsible for significant number of hospital admissions. Thus, the present study emphasises on the need and importance of an effective pharmacovigilance programme.Methods: A prospective study was undertaken in a 183 cases tertiary care teaching hospital of India. Male to female ratio, most common class of drug, individual drug causing ACDR, common types of ACDRs Parameters were studied. Other Parameters like Causality, preventability and severe or non-severe reactions were analyzed.Results: Majority of the patients (48%) with CADR belonged to the age group 25-44 followed by 45-64 (28%). Most frequent adverse cutaneous drug reactions reported were Urticaria (40%), Maculopapular rash (25%) & Fixed drug eruptions (21%) in decreasing order of frequency. Majority of reactions (96%) were Bizarre/Unpredictable in nature. As a group, antimicrobials (46%) were most frequently associated with CADR followed by NSAIDs (31%) and antiepileptics (11%). Most of the reactions (93%) were mild-moderate and probable (77%) in nature. Approximately 60% of ACDRs reported in this study were preventable.Conclusions: There was slight male preponderance except acneiform eruptions. Cotrimoxazole being the most common offending drug then after Ibuprofen, Phenytoin among the anti-inflammatory, analgesics, antiepileptics class. Causality assessment resulted in high score 77% of probable category.
Cementless bipolar hemiarthroplasty for displaced fracture neck of femur with modular hydroxyapatite coated stem in elderly with cardiopulmonary co-morbidities
Authors: Bhat Towseef Ahmad , Ali Zameer , Gulzar Amara , Mirza Furqaan 
Published: April 2017

Background: Bipolar hemiarthroplasty is one of the common procedures done for fracture neck of femur in elderly. Debate about Cemented or cementless is still on. Cementing increases perioperative mortality by causing significant hemodynamic changes or embolization. Patients with cardiopulmonary complications are at higher risk of cementing complications. We report our series of 31 cases of fracture neck of femur with cardiopulmonary diseases operated over 2 years with modular cementless bipolar hemiarthroplasty.Methods: 31 elderly patients with cardiopulmonary co-morbidities (age=75 to 97 years) with displaced femoral neck fractures were operated between January 2011 to December 2013. Cementless bipolar hemiarthroplasty using hydroxyapatite coated stem was done by single surgeon using same implant in all the patients through anterolateral approach. Clinical and radiological follow-up was done with mean follow up of 54 months (36-72 months).Results: Total 31 cases with cardiopulmonary comorbidities were operated. The average follow up was 54 months. No intraoperative mortality was seen. 3 patients had splitting of femur during canal preparation. 1 patient died due to cardiac arrest in the post-operative period. 1 patient had surgical site infection. 3 patients had hemodynamic abnormalities and 1 patient had significant limb length discripency (1.5 cm). 2 patients died in the follow up because of comorbidities. 27 patients reached to pre-injury status with average harris hip score of 86 at final follow up.Conclusions: Cementless bipolar hemiarthroplasty with hydroxyapatite coated stem is a good option for femoral neck fractures in elderly patients with cardiopulmonary complications without risking the harmful effects of cementing.