bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Violence Within: Canadian Modern Statehood and the Pan-territorial Residential School System Ideal
Authors: Murray Karen Bridget 
Published: May 2017

AbstractDrawing upon Walter Benjamin's “principle of montage,” this article excavates the political salience of what is referred to herein as the residential school system's “pan-territorial ideal.” The pan-territorial ideal materialized in 1930 with the opening of the Shubenacadie Residential School in the Maritimes, the system's final frontier. It was envisioned, forged and secured, in part, with the overt understanding that so called Indian education could be used as a vector of violence to control Indigenous peoples and their lands. This history clashes with dominant narratives that interpret residential school system violence as the product of mismanagement and neglect. From the early days of Confederation to its almost full legal autonomy from Britain in the early twentieth century and beyond, the Dominion's pursuit of the pan-territorial vision involved the selective harnessing of the residential school system as a field of state-sanctioned force to quell Indigenous resistance. In this, residential school violence cannot be reduced to a deviation from the norm. In crucial respects, it was an inherent feature of the system and Canadian modern statehood itself.
Efficient Kriging surrogate modeling approach for system reliability analysis
Authors: Hu Zhen , Nannapaneni Saideep , Mahadevan Sankaran 
Published: May 2017

AbstractCurrent limit state surrogate modeling methods for system reliability analysis usually build surrogate models for failure modes individually or build composite limit states. In practical engineering applications, multiple system responses may be obtained from a single setting of inputs. In such cases, building surrogate models individually will ignore the correlation between different system responses and building composite limit states may be computationally expensive because the nonlinearity of composite limit state is usually higher than individual limit states. This paper proposes a new efficient Kriging surrogate modeling approach for system reliability analysis by constructing composite Kriging surrogates through selection of Kriging surrogates constructed individually and Kriging surrogates built based on singular value decomposition. The resulting composite surrogate model will combine the advantages of both types of Kriging surrogate models and thus reduce the number of required training points. A new stopping criterion and a new surrogate model refinement strategy are proposed to further improve the efficiency of this approach. The surrogate models are refined adaptively with high accuracy near the active failure boundary until the proposed new stopping criterion is satisfied. Three numerical examples including a series, a parallel, and a combined system are used to demonstrate the effectiveness of the proposed method.
Efficient simulation of threshold behavior
Authors: Kohler Dominic , Müller Johannes , Obst Birgit , Wever Utz 
Published: May 2017

AbstractThe paper discusses a new method for the propagation of risk levels. This discretization takes place by considering the phase space of the state and its subdivision into boxes. In each time step, the method computes the probability of the state being in one box. We start with a variety of technical and physical problems by showing how discrete modeling under uncertainties is technically meaningful and often even problem inherent. Then we select the technical problem of contaminant spread in water grids, to which we apply the method of risk-level propagation in depth. Extensions of the method such as modeling of contaminant mixing at junctions in the discretized phase space and the applicability of conservation laws arise naturally along these lines and are discussed in the context of the general theory.
The WTO–TRIPS Flexibilities on Public Health: A Critical Appraisal of the East African Community Regional Framework
Authors: SOYEJU OLUFEMI , WABWIRE JOSHUA 
Published: May 2017

AbstractOver the years, many developing countries have attempted to make policies utilizing the WTO–TRIPS flexibilities to address the public health needs of their populations. A common strategic trend in these policies has been the tendency to attempt to increase access to medicines through price reduction, achieved by weakening patent protection. This paper, using the policy that has recently been adopted by the East African Community (EAC) member states as a case study, demonstrates the inappropriateness of this strategy. The core argument is that weakening patent protection will hinder further research and invention, which are necessary to ensure the availability of medicines. For developing countries, especially those in Africa, such as the EAC member states, the problem is aggravated by the fact that pharmaceuticals, due to commercial considerations, have already ignored investing in developing medicines for diseases predominant in these countries, hence the need to strengthen rather than weaken, patent protection.
Application of bivariate negative binomial regression model in analysing insurance count data
Authors: Liu Feng , Pitt David 
Published: May 2017

AbstractIn this paper we analyse insurance claim frequency data using the bivariate negative binomial regression (BNBR) model. We use general insurance data on claims from simple third-party liability insurance and comprehensive insurance. We find that bivariate regression, with its capacity for modelling correlation between the two observed claim counts, provides both a superior fit and out-of-sample prediction compared with the more common practice of fitting univariate negative binomial regression models separately to each claim type. Noting the complexity of BNBR models and their potential for a large number of parameters, we explore the use of model shrinkage methodology, namely the least absolute shrinkage and selection operator (Lasso) and ridge regression. We find that models estimated using shrinkage methods outperform the ordinary likelihood-based models when being used to make predictions out-of-sample. We find that the Lasso performs better than ridge regression as a method of shrinkage.
Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity
Authors: Shi Yuheng , Yuan Bofeng , Zhu Wenting , Zhang Rui , Li Lin , Hao Xiaojing , Chen She , Hou Fajian 
Published: May 2017

AbstractInnate immunity plays a pivotal role in virus infection. RIG-I senses viral RNA and initiates an effective innate immune response for type I interferon production. To transduce RIG-I-mediated antiviral signalling, a mitochondrial protein MAVS forms prion-like aggregates to activate downstream kinases and transcription factors. However, the activation mechanism of RIG-I is incompletely understood. Here we identify two ubiquitin enzymes Ube2D3 and Ube2N through chromatographic purification as activators for RIG-I on virus infection. We show that together with ubiquitin ligase Riplet, Ube2D3 promotes covalent conjugation of polyubiquitin chains to RIG-I, while Ube2N preferentially facilitates production of unanchored polyubiquitin chains. In the presence of these polyubiquitin chains, RIG-I induces MAVS aggregation directly on the mitochondria. Our data thus reveal two essential polyubiquitin-mediated mechanisms underlying the activation of RIG-I and MAVS for triggering innate immune signalling in response to viral infection in cells.
Probing nanoscale oxygen ion motion in memristive systems
Authors: Yang Yuchao , Zhang Xiaoxian , Qin Liang , Zeng Qibin , Qiu Xiaohui , Huang Ru 
Published: May 2017

AbstractIon transport is an essential process for various applications including energy storage, sensing, display, memory and so on, however direct visualization of oxygen ion motion has been a challenging task, which lies in the fact that the normally used electron microscopy imaging mainly focuses on the mass attribute of ions. The lack of appropriate understandings and analytic approaches on oxygen ion motion has caused significant difficulties in disclosing the mechanism of oxides-based memristors. Here we show evidence of oxygen ion migration and accumulation in HfO2 by in situ measurements of electrostatic force gradient between the probe and the sample, as systematically verified by the charge duration, oxygen gas eruption and controlled studies utilizing different electrolytes, field directions and environments. At higher voltages, oxygen-deficient nano-filaments are formed, as directly identified employing a CS-corrected transmission electron microscope. This study could provide a generalized approach for probing ion motions at the nanoscale.
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
Authors: Jiang Yu , Qian Fenghui , Yang Junjie , Liu Yingmiao , Dong Feng , Xu Chongmao , Sun Bingbing , Chen Biao , Xu Xiaoshu , Li Yan , Wang Renxiao , Yang Sheng 
Published: May 2017

AbstractCorynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. glutamicum. Here we report a Francisella novicida (Fn) CRISPR-Cpf1-based genome-editing method for C. glutamicum. CRISPR-Cpf1, combined with single-stranded DNA (ssDNA) recombineering, precisely introduces small changes into the bacterial genome at efficiencies of 86–100%. Large gene deletions and insertions are also obtained using an all-in-one plasmid consisting of FnCpf1, CRISPR RNA, and homologous arms. The two CRISPR-Cpf1-assisted systems enable N iterative rounds of genome editing in 3N+4 or 3N+2 days. A proof-of-concept, codon saturation mutagenesis at G149 of γ-glutamyl kinase relieves L-proline inhibition using Cpf1-assisted ssDNA recombineering. Thus, CRISPR-Cpf1-based genome editing provides a highly efficient tool for genetic engineering of Corynebacterium and other bacteria that cannot utilize the Sp CRISPR-Cas9 system.
The clinical impact of copy number variants in inherited bone marrow failure syndromes
Authors: Waespe Nicolas , Dhanraj Santhosh , Wahala Manju , Tsangaris Elena , Enbar Tom , Zlateska Bozana , Li Hongbing , Klaassen Robert J. , Fernandez Conrad V. , Cuvelier Geoff D. E. , Wu John K. , Pastore Yves D. , Silva Mariana , Lipton Jeffrey H. , Brossard Joseé , Michon Bruno , Abish Sharon , Steele MacGregor , Sinha Roona , Belletrutti Mark J. , Breakey Vicky R. , Jardine Lawrence , Goodyear Lisa , Kofler Liat , Cada Michaela , Sung Lillian , Shago Mary , Scherer Stephen W. , Dror Yigal 
Published: May 2017

AbstractInherited bone marrow failure syndromes comprise a genetically heterogeneous group of diseases with hematopoietic failure and a wide array of physical malformations. Copy number variants were reported in some inherited bone marrow failure syndromes. It is unclear what impact copy number variants play in patients evaluated for a suspected diagnosis of inherited bone marrow failure syndromes. Clinical and genetic data of 323 patients from the Canadian Inherited Marrow Failure Registry from 2001 to 2014, who had a documented genetic work-up, were analyzed. Cases with pathogenic copy number variants (at least 1 kilobasepairs) were compared to cases with other mutations. Genotype-phenotype correlations were performed to assess the impact of copy number variants. Pathogenic nucleotide-level mutations were found in 157 of 303 tested patients (51.8%). Genome-wide copy number variant analysis by single-nucleotide polymorphism arrays or comparative genomic hybridization arrays revealed pathogenic copy number variants in 11 of 67 patients tested (16.4%). In four of these patients, identification of copy number variant was crucial for establishing the correct diagnosis as their clinical presentation was ambiguous. Eight additional patients were identified to harbor pathogenic copy number variants by other methods. Of the 19 patients with pathogenic copy number variants, four had compound-heterozygosity of a copy number variant with a nucleotide-level mutation. Pathogenic copy number variants were associated with more extensive non-hematological organ system involvement (p = 0.0006), developmental delay (p = 0.006) and short stature (p = 0.04) compared to nucleotide-level mutations. In conclusion, a significant proportion of patients with inherited bone marrow failure syndromes harbor pathogenic copy number variants which were associated with a more extensive non-hematological phenotype in this cohort. Patients with a phenotype suggestive of inherited bone marrow failure syndromes but without identification of pathogenic nucleotide-level mutations should undergo specific testing for copy number variants.
The response of a boreal deep-sea sponge holobiont to acute thermal stress
Authors: Strand R. , Whalan S. , Webster N. S. , Kutti T. , Fang J. K. H. , Luter H. M. , Bannister R. J. 
Published: May 2017

AbstractEffects of elevated seawater temperatures on deep-water benthos has been poorly studied, despite reports of increased seawater temperature (up to 4 °C over 24 hrs) coinciding with mass mortality events of the sponge Geodia barretti at Tisler Reef, Norway. While the mechanisms driving these mortality events are unclear, manipulative laboratory experiments were conducted to quantify the effects of elevated temperature (up to 5 °C, above ambient levels) on the ecophysiology (respiration rate, nutrient uptake, cellular integrity and sponge microbiome) of G. barretti. No visible signs of stress (tissue necrosis or discolouration) were evident across experimental treatments; however, significant interactive effects of time and treatment on respiration, nutrient production and cellular stress were detected. Respiration rates and nitrogen effluxes doubled in responses to elevated temperatures (11 °C & 12 °C) compared to control temperatures (7 °C). Cellular stress, as measured through lysosomal destabilisation, was 2–5 times higher at elevated temperatures than for control temperatures. However, the microbiome of G. barretti remained stable throughout the experiment, irrespective of temperature treatment. Mortality was not evident and respiration rates returned to pre-experimental levels during recovery. These results suggest other environmental processes, either alone or in combination with elevated temperature, contributed to the mortality of G. barretti at Tisler reef.
Everyday cognition scales are related to cognitive function in the early stage of probable Alzheimer’s disease and FDG-PET findings
Authors: Hsu Jung-Lung , Hsu Wen-Chuin , Chang Chiung-Chih , Lin Kun-Ju , Hsiao Ing-Tsung , Fan Yen-Chun , Bai Chyi-Huey 
Published: May 2017

AbstractWe applied the Everyday Cognition (ECog) scale in normal aging adults and patients with Alzheimer’s disease (AD) to investigate associations between neuropsychological tests and neuroimaging markers. A total of 160 normal aging adults and 40 patients with the early stage of probable AD were included. Neuropsychological performance was assessed using the Consortium to Establish a Registry for Alzheimer’s Disease Neuropsychological Assessment Battery (CERAD-NAB). 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) scans were used to measure AD-related hypometabolism. Nonparametric Spearman correlation analysis was used to study associations between ECog and z-transformed total CERAD-NAB scores in both groups. The results revealed a significant correlation between total ECog and CERAD-NAB scores (rho = −0.28, p < 0.01), and category verbal fluency test with the executive domain of the ECog scale (rho = −0.20, p < 0.01). The CERAD-NAB scores were also significantly correlated with AD-related hypometabolism (rho = −0.49, p < 0.01). The memory domain of the ECog scale was significantly correlated with FDG uptake in the angular gyrus and posterior cingulum gyrus (rho = −0.41 and −0.46, P < 0.01). In conclusion, both total and memory domain ECog scores were correlated with the neuropsychological tests and neuroimaging biomarkers.
A nanofabricated, monolithic, path-separated electron interferometer
Authors: Agarwal Akshay , Kim Chung-Soo , Hobbs Richard , Dyck Dirk van , Berggren Karl K. 
Published: May 2017

AbstractProgress in nanofabrication technology has enabled the development of numerous electron optic elements for enhancing image contrast and manipulating electron wave functions. Here, we describe a modular, self-aligned, amplitude-division electron interferometer in a conventional transmission electron microscope. The interferometer consists of two 45-nm-thick silicon layers separated by 20 μm. This interferometer is fabricated from a single-crystal silicon cantilever on a transmission electron microscope grid by gallium focused-ion-beam milling. Using this interferometer, we obtain interference fringes in a Mach-Zehnder geometry in an unmodified 200 kV transmission electron microscope. The fringes have a period of 0.32 nm, which corresponds to the [1̄1̄1] lattice planes of silicon, and a maximum contrast of 15%. We use convergent-beam electron diffraction to quantify grating alignment and coherence. This design can potentially be scaled to millimeter-scale, and used in electron holography. It could also be applied to perform fundamental physics experiments, such as interaction-free measurement with electrons.
The intestinal tuft cell nanostructure in 3D
Authors: Hoover Ben , Baena Valentina , Kaelberer Melanie M. , Getaneh Feven , Chinchilla Skarleth , Bohórquez Diego V. 
Published: May 2017

AbstractOnce referred to as “peculiar,” tuft cells are enigmatic epithelial cells. Here, we reasoned that future functional studies could be derived from a complete account of the tuft cell ultrastructure. We identified and documented the volumetric ultrastructure at nanometer resolution (4–5 nm/pixel) of specific intestinal tuft cells. The techniques used were Serial Block-Face (SBF) and Automated Tape-collecting Ultra-Microtome (ATUM) Scanning Electron Microscopy (SEM). Our results exposed a short (~15 µm) basal cytoplasmic process devoid of secretory vesicles. Volume rendering of serial sections unveiled several thin cytospinules (~1 µm). These cytospinules project from the tuft cell into the nuclei of neighboring epithelial cells. Volume rendering also revealed within the tuft cell an elegant network of interconnected tubules. The network forms a passage from the base of the microvilli to the rough endoplasmic reticulum. Based on their location and microanatomy, the tuft cells’ cytospinules, and tubular network, might facilitate the exchange of molecular cargo with nuclei of neighboring cells, and the gut lumen.
Biliverdin Reductase inhibitors did not improve severe unconjugated hyperbilirubinemia in vivo
Authors: van Dijk Remco , Aronson Sem J. , de Waart Dirk R. , van de Graaf Stan F. , Duijst Suzanne , Seppen Jurgen , Elferink Ronald Oude , Beuers Ulrich , Bosma Piter J. 
Published: May 2017

AbstractWe aimed to identify potent biliverdin reductase (BVRA) inhibitors as a novel concept for the treatment of severe unconjugated hyperbilirubinemia. 1280 FDA-approved compounds were screened in vitro for their ability to inhibit human and rat BVRA activity ﻿and 2﻿6 compounds were identified as BVRA inhibitors﻿. Montelukast and Disulfiram were sel﻿ected as potentially clinically applicable drug﻿s and tested to reduce serum unconjugated bilirubin (UCB) levels in the Ugt1a1-deficient rat, a model for chronic unconjugated hyperbilirubinemia. Oral administration of Disulfiram was toxic in the Ugt1a1-deficient rat (weight loss, transaminase elevation). Oral Montelukast administration led to low serum concentrations and did not alter serum UCB levels. Intraperitoneal injections of Mont﻿elukast resulted in concentrations up to 110 μmol/L in serum and 400 μmol/L in the liver. Still, serum UCB levels remained unaltered. This first study on biliverdin reductase inhibition as a novel concept for treatment of unconjugated hyperbilirubinemia identified putative in vitro BVRA inhibitors. Montelukast, the clinically most suitable inhibitor, did not result in reduction of serum UCB in the Ugt1a1-deficient rat. The proposed treatment strategy will not result in amelioration of severe unconjugated hyperbilirubinemia in humans without the identification or development of more potent BVRA inhibitors.
Alkali-created rich properties in grapheme nanoribbons: Chemical bondings
Authors: Lin Yu-Tsung , Lin Shih-Yang , Chiu Yu-Huang , Lin Ming-Fa 
Published: May 2017

AbstractThe alkali-adsorbed graphene nanoribbons exhibit the feature-rich electronic and magnetic properties. From the first-principles calculations, there are only few adatom-dominated conduction bands, and the other conduction and valence bands are caused by carbon atoms. A lot of free electrons are revealed in the occupied alkali- and carbon-dependent conduction bands. Energy bands are sensitive to the concentration, distribution and kind of adatom and the edge structure, while the total linear free carrier density only relies on the first one. These mainly arise from a single s − 2p
z
orbital hybridization in the adatom-carbon bond. Specifically, zigzag systems can present the anti-ferromagnetic ordering across two edges, ferromagnetic ordering along one edge and non-magnetism, being reflected in the edge-localized energy bands with or without spin splitting. The diverse energy dispersions contribute many special peaks in density of states. The critical chemical bonding and the distinct spin configuration could be verified from the experimental measurements.
Performances of Kevlar and Polyethylene as radiation shielding on-board the International Space Station in high latitude radiation environment
Authors: Narici Livio , Casolino Marco , Di Fino Luca , Larosa Marianna , Picozza Piergiorgio , Rizzo Alessandro , Zaconte Veronica 
Published: May 2017

AbstractPassive radiation shielding is a mandatory element in the design of an integrated solution to mitigate the effects of radiation during long deep space voyages for human exploration. Understanding and exploiting the characteristics of materials suitable for radiation shielding in space flights is, therefore, of primary importance. We present here the results of the first space-test on Kevlar and Polyethylene radiation shielding capabilities including direct measurements of the background baseline (no shield). Measurements are performed on-board of the International Space Station (Columbus modulus) during the ALTEA-shield ESA sponsored program. For the first time the shielding capability of such materials has been tested in a radiation environment similar to the deep-space one, thanks to the feature of the ALTEA system, which allows to select only high latitude orbital tracts of the International Space Station. Polyethylene is widely used for radiation shielding in space and therefore it is an excellent benchmark material to be used in comparative investigations. In this work we show that Kevlar has radiation shielding performances comparable to the Polyethylene ones, reaching a dose rate reduction of 32 ± 2% and a dose equivalent rate reduction of 55 ± 4% (for a shield of 10 g/cm2).