bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enrofloxacin enhances the effects of chemotherapy in canine osteosarcoma cells with mutant and wild‐type p53
Authors: York D. , Withers S. S. , Watson K. D. , Seo K. W. , Rebhun R. B. 
Published: June 2016

AbstractAdjuvant chemotherapy improves survival time in dogs receiving adequate local control for appendicular osteosarcoma, but most dogs ultimately succumb to metastatic disease. The fluoroquinolone antibiotic enrofloxacin has been shown to inhibit survival and proliferation of canine osteosarcoma cells in vitro. Others have reported that fluoroquinolones may modulate cellular responses to DNA damaging agents and that these effects may be differentially mediated by p53 activity. We therefore determined p53 status and activity in three canine osteosarcoma cell lines and examined the effects of enrofloxacin when used alone or in combination with doxorubicin or carboplatin chemotherapy. Moresco and Abrams canine osteosarcoma cell lines contained mutations in p53, while no mutations were identified in the D17 cells or in a normal canine osteoblast cell line. The addition of enrofloxacin to either doxorubicin or carboplatin resulted in further reductions in osteosarcoma cell viability; this effect was apparent regardless of p53 mutational status or downstream activity.
Confirmation of a Campylobacteriosis Outbreak Associated with Chicken Liver Pâté Using PFGE and WGS
Authors: Lahti E. , Löfdahl M. , Ågren J. , Hansson I. , Olsson Engvall E. 
Published: June 2016

SummaryIn May 2012, an outbreak of campylobacteriosis occurred in southern Sweden at a wedding reception affecting 44 persons. A total of 17 cases were notified (13 were culture positive for Campylobacter spp.). Epidemiological investigation suspected chicken liver pâté as the source of infection. The liver pâté had been deliberately undercooked, lightly fried to keep the right texture and mixed with spices. Campylobacter isolates from six cases as well as three Campylobacter isolates from chicken flocks previously raised by the producer delivering the liver were subtyped using pulsed‐field gel electrophoresis and whole‐genome sequencing. Indistinguishable PFGE profiles were identified among five human and one chicken C. jejuni isolates as well among the two C. coli isolates, one from a human case and one from a chicken. WGS supported the PFGE findings; the six C. jejuni isolates belonged to one cluster. All these six isolates were of MLST type ST 50 (ST‐CC 21). This study highlights the importance of a combination of strict biosecurity at the flock‐level as well as adequate cooking of chicken liver to prevent transmission of Campylobacter to humans.
Reviewer Acknowledgements
Authors: Young James 
Published: June 2016

International Journal of Social Science Studies (IJSSS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether IJSSS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue. Reviewers for Volume 4, Number 7 Abdul Azim Akhtar Ahmet Yıkmış Ali Massoud Anna Maria Mouza Chih-Chien Huang Dipankar Chatterjee Hanaa Youssef Shaarawy Henry Poduthas JEHU ONYEKWERE NNAJI, Jenny Tonsing Jianquan Cheng Julia M. Mack K.O. Aramide, Katja Eman Lisa Cherkassky Małgorzata Haładewicz Michael Brooks Mohammad Naji Shah Mohammadi Muharrem EKŞİ Oluwagbemiga Paul Agboola Patricia Wiener Paula Hanasz Priyono Rachita Shrivastava Roy Remigiusz Kijak Said Aldhafri Sanro Serpa Sharif Alghazo Zafer İBRAHİMOĞLU Zlatan Begić James Young Editorial Assistant On behalf of, The Editorial Board of International Journal of Social Science Studies Redfame Publishing 9450 SW Gemini Dr. #99416 Beaverton, OR 97008, USA URL: http://ijsss.redfame.com
The role of transient receptor potential channels in hypertension and metabolic vascular damage
Authors: Zhu Zhiming , Xiong Shiqiang , Li Qiang 
Published: June 2016

New Findings

What is the topic of this review?
Transient receptor potential (TRP) channels are highly implicated in the pathogenesis of hypertension and the regulation of metabolism.

What advances does it highlight?
Dysfunction of TRP channels leads to hypertension and metabolic disorders. Elucidating the role of TRP channels in hypertension and metabolic vascular damage would facilitate the design of novel target therapeutics for these intractable diseases.

Intracellular Ca2+ homeostasis is critical for vascular function and the regulation of metabolism. Metabolic disorders are major risk factors for hypertension. A family of transient receptor potential (TRP) channels plays an important role in the regulation of cellular calcium signalling and cardiometabolic function. Emerging evidence indicates that TRP channels are highly implicated in the pathogenesis of hypertension and metabolic disorders. Dysfunction of TRP channels leads to hypertension and metabolic dysfunction. Activation of certain subtypes of TRP channels could attenuate metabolic vascular damage and alleviate hypertension. Therefore, elucidating the role of TRP channels in the physiological state and in cardiometabolic diseases will facilitate the design of novel targeted therapeutics for these intractable diseases.
Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens
Authors: Leber Amy L. , Everhart Kathy , Balada-Llasat Joan-Miquel , Cullison Jillian , Daly Judy , Holt Sarah , Lephart Paul , Salimnia Hossein , Schreckenberger Paul C. , DesJarlais Sharon , Reed Sharon L. , Chapin Kimberle C. , LeBlanc Lindsay , Johnson J. Kristie , Soliven Nicole L. , Carroll Karen C. , Miller Jo-Anne , Dien Bard Jennifer , Mestas Javier , Bankowski Matthew , Enomoto Tori , Hemmert Andrew C. , Bourzac Kevin M. 
Published: June 2016

ABSTRACT

Rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. Comprehensive testing of cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. These methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. The FilmArray Meningitis/Encephalitis (ME) Panel is a multiplexed
in vitro
diagnostic test for the simultaneous, rapid (∼1-h) detection of 14 pathogens directly from CSF specimens:
Escherichia coli
K1,
Haemophilus influenzae
,
Listeria monocytogenes
,
Neisseria meningitidis
,
Streptococcus pneumoniae
,
Streptococcus agalactiae
, cytomegalovirus, enterovirus, herpes simplex virus 1 and 2, human herpesvirus 6, human parechovirus, varicella-zoster virus, and
Cryptococcus neoformans
/
Cryptococcus gattii
. We describe a multicenter evaluation of 1,560 prospectively collected CSF specimens with performance compared to culture (bacterial analytes) and PCR (all other analytes). The FilmArray ME Panel demonstrated a sensitivity or positive percentage of agreement of 100% for 9 of 14 analytes. Enterovirus and human herpesvirus type 6 had agreements of 95.7% and 85.7%, and
L. monocytogenes
and
N. meningitidis
were not observed in the study. For
S. agalactiae
, there was a single false-positive and false-negative result each, for a sensitivity and specificity of 0 and 99.9%, respectively. The specificity or negative percentage of agreement was 99.2% or greater for all other analytes. The FilmArray ME Panel is a sensitive and specific test to aid in diagnosis of ME. With use of this comprehensive and rapid test, improved patient outcomes and antimicrobial stewardship are anticipated.
Superiority of West Nile Virus RNA Detection in Whole Blood for Diagnosis of Acute Infection
Authors: Lustig Yaniv , Mannasse Batya , Koren Ravit , Katz-Likvornik Shiri , Hindiyeh Musa , Mandelboim Michal , Dovrat Sara , Sofer Danit , Mendelson Ella 
Published: June 2016

ABSTRACT
The current diagnosis of West Nile virus (WNV) infection is primarily based on serology, since molecular identification of WNV RNA is unreliable due to the short viremia and absence of detectable virus in cerebrospinal fluid (CSF). Recent studies have shown that WNV RNA can be detected in urine for a longer period and at higher concentrations than in plasma. In this study, we examined the presence of WNV RNA in serum, plasma, whole-blood, CSF, and urine samples obtained from patients diagnosed with acute WNV infection during an outbreak which occurred in Israel in 2015. Our results demonstrate that 33 of 38 WNV patients had detectable WNV RNA in whole blood at the time of diagnosis, a higher rate than in any of the other sample types tested. Overall, whole blood was superior to all other samples, with 86.8% sensitivity, 100% specificity, 100% positive predictive value, and 83.9% negative predictive value. Interestingly, WNV viral load in urine was higher than in whole blood, CSF, serum, and plasma despite the lower sensitivity than that of whole blood. This study establishes the utility of whole blood in the routine diagnosis of acute WNV infection and suggests that it may provide the highest sensitivity for WNV RNA detection in suspected cases.
Comparison of peripheral and central capillary refill time in febrile children presenting to a paediatric emergency department and its utility in identifying children with serious bacterial infection
Authors: de Vos-Kerkhof Evelien , Krecinic Tarik , Vergouwe Yvonne , Moll Henriëtte A , Nijman Ruud G , Oostenbrink Rianne 
Published: June 2016

ObjectiveTo determine the agreement between peripheral and central capillary refill time (pCRT/cCRT) and their diagnostic values for detecting serious bacterial infection (SBI) in febrile children attending the paediatric emergency department (ED).DesignProspective observational study.SettingPaediatric ED, Erasmus Medium Care-Sophia Children's hospital, the Netherlands.Patients1193 consecutively included, previously healthy, febrile children (1 month–16 years) with both pCRT measurements and cCRT measurements available. SBI diagnosis was based on abnormal radiographic findings and/or positive cultures from normally sterile locations in addition to clinical criteria.Main outcome measuresAgreement between pCRT and cCRT (Cohen's κ), overall and stratified for age and body temperature. The diagnostic value of pCRT and cCRT for SBI was assessed with logistic regression.ResultsOverall agreement was 0.35 (95% CI 0.27 to 0.43; considered ‘fair’). Although not significant, agreement was lower in children aged 1–<5 years (κ: 0.15 (95% CI 0.04 to 0.27)) and decreased with higher body temperatures with κ ranging from 0.55 (95% CI 0.32 to 0.79) for temperature <37.5°C to 0.21 (95% CI 0.07 to 0.34) for temperature >39.5°C. Abnormal pCRT (>2 s) was observed in 153 (12.8%; 95% CI 10.9% to 14.7%) and abnormal cCRT in 55 (4.6%; 95% CI 3.4% to 5.8%) children. The OR of abnormal pCRT (>2 s) for predicting SBI was 1.10 (95% CI 0.65 to 1.84). For abnormal cCRT (>2 s), the OR was 0.43 (95% CI 0.13 to 1.39).ConclusionsThe pCRT and cCRT values showed only fair agreement in a general population of febrile children at the ED, and no significant association with age or body temperature was found. Only a small part of febrile children at risk for serious infections at the ED show abnormal CRT values. Both abnormal pCRT and cCRT (defined as >2 s) performed poorly and were non-significant in this study detecting SBI in a general population of febrile children.
Physical activity, sedentary behaviors and the incidence of type 2 diabetes mellitus: the Multi-Ethnic Study of Atherosclerosis (MESA)
Authors: Joseph Joshua J , Echouffo-Tcheugui Justin B , Golden Sherita H , Chen Haiying , Jenny Nancy Swords , Carnethon Mercedes R , Jacobs David , Burke Gregory L , Vaidya Dhananjay , Ouyang Pamela , Bertoni Alain G 
Published: June 2016

BackgroundThe association between physical activity (PA), sedentary behavior, and incident diabetes has been assessed in whites but is less well investigated in multiethnic populations.ObjectiveTo assess the association between PA, sedentary behavior, and incident diabetes in the Multi-Ethnic Study of Atherosclerosis.Research design and methodsIncident diabetes was assessed among adults without prevalent baseline diabetes (2000–2002) at 5 in-person examinations between 2002 and 2012. Baseline PA (moderate, vigorous, and exercise-specific; metabolic equivalents of task-hours/week) and sedentary behaviors (television watching, reading; hours/day) were assessed by questionnaire. HRs were estimated using Cox proportional hazard models.ResultsAmong 5829 adults (mean age 61.8 years, 54% female, 42% white, 12% Chinese-American, 26% African-American, 21% Hispanic-American), there were 655 incident diabetes cases (median follow-up 11.1 years). After adjustment, diabetes risk was lower in those with brisk or striding compared with none or casual walking pace (HR 0.67; 95% CI 0.54 to 0.84), higher levels of exercise PA (HR for highest vs lowest quartile 0.79; 95% CI 0.63 to 0.98), and any compared with no vigorous PA (HR 0.79; 95% CI 0.66 to 0.95). Race/ethnicity influenced the association of walking pace, exercise PA, and any vigorous PA on diabetes risk, which was only significant among whites. Total leisure sedentary behaviors (HR for highest vs lowest quartile 1.65; 95% CI 1.26 to 2.14) and television watching (HR for highest vs lowest quartile 2.68; 95% CI 1.38 to 5.21) were significantly associated with diabetes risk in multiethnic analyses and were influenced by race/ethnicity.ConclusionsThese results confirm the importance of PA and sedentary behavior on diabetes risk in a multiethnic population and demonstrate potential variations across race/ethnic groups.
Validation of a performance checklist for ultrasound-guided internal jugular central lines for use in procedural instruction and assessment
Authors: Hartman Nicholas , Wittler Mary , Askew Kim , Hiestand Brian , Manthey David 
Published: June 2016

ABSTRACTPurpose of the studyTools created to measure procedural competency must be tested in their intended environment against an established standard in order to be validated. We previously created a checklist for ultrasound-guided internal jugular central venous catheter (US IJ CVC) insertion using the modified Delphi method. We sought to further validate the checklist tool for use in an educational environment.Study designThis is a cohort study involving 15 emergency medicine interns being evaluated on their skill in US IJ CVC placement. We compared the checklist tool with a modified version of a clinically validated global rating scale (GRS) for procedural performance.ResultsThe correlation between the GRS tool and the checklist tool was excellent, with a correlation coefficient (Pearson’s r) of 0.90 (p&lt;0.0001).ConclusionsThis checklist represents a useful tool for measuring procedural competency.
Whole-Exome Sequencing of Salivary Gland Mucoepidermoid Carcinoma
Authors: Kang Hyunseok , Tan Marietta , Bishop Justin A. , Jones Siân , Sausen Mark , Ha Patrick K. , Agrawal Nishant 
Published: June 2016

Abstract
Purpose: Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. To explore the genetic origins of MEC, we performed systematic genomic analyses of these tumors.
Experimental Design: Whole-exome sequencing and gene copy-number analyses were performed for 18 primary cancers with matched normal tissue. FISH was used to determine the presence or absence of the MECT1-MAML2 translocation in 17 tumors.
Results: TP53 was the most commonly mutated gene in MEC (28%), and mutations were found only in intermediate- and high-grade tumors. Tumors with TP53 mutations had more mutations overall than tumors without TP53 mutations (P = 0.006). POU6F2 was the second most frequently mutated gene, found in three low-grade MECs with the same in-frame deletion. Somatic alterations in IRAK1, MAP3K9, ITGAL, ERBB4, OTOGL, KMT2C, and OBSCN were identified in at least two of the 18 tumors sequenced. FISH analysis confirmed the presence of the MECT1-MAML2 translocation in 15 of 17 tumors (88%).
Conclusions: Through these integrated genomic analyses, MECT1-MAML2 translocation and somatic TP53 and POU6F2 mutations appear to be the main drivers of MEC. Clin Cancer Res; 23(1); 283–8. ©2016 AACR.
Prevention of Carcinogen-Induced Oral Cancer by Sulforaphane
Authors: Bauman Julie E. , Zang Yan , Sen Malabika , Li Changyou , Wang Lin , Egner Patricia A. , Fahey Jed W. , Normolle Daniel P. , Grandis Jennifer R. , Kensler Thomas W. , Johnson Daniel E. 
Published: June 2016

Abstract
Chronic exposure to carcinogens represents the major risk factor for head and neck squamous cell carcinoma (HNSCC). Beverages derived from broccoli sprout extracts (BSE) that are rich in glucoraphanin and its bioactive metabolite sulforaphane promote detoxication of airborne pollutants in humans. Herein, we investigated the potential chemopreventive activity of sulforaphane using in vitro models of normal and malignant mucosal epithelial cells and an in vivo model of murine oral cancer resulting from the carcinogen 4-nitroquinoline-1-oxide (4NQO). Sulforaphane treatment of Het-1A, a normal mucosal epithelial cell line, and 4 HNSCC cell lines led to dose- and time-dependent induction of NRF2 and the NRF2 target genes NQO1 and GCLC, known mediators of carcinogen detoxication. Sulforaphane also promoted NRF2-independent dephosphorylation/inactivation of pSTAT3, a key oncogenic factor in HNSCC. Compared with vehicle, sulforaphane significantly reduced the incidence and size of 4NQO-induced tongue tumors in mice. A pilot clinical trial in 10 healthy volunteers evaluated the bioavailability and pharmacodynamic activity of three different BSE regimens, based upon urinary sulforaphane metabolites and NQO1 transcripts in buccal scrapings, respectively. Ingestion of sulforaphane-rich BSE demonstrated the greatest, most consistent bioavailability. Mucosal bioactivity, defined as 2-fold or greater upregulation of NQO1 mRNA, was observed in 6 of 9 evaluable participants ingesting glucoraphanin-rich BSE; 3 of 6 ingesting sulforaphane-rich BSE; and 3 of 9 after topical-only exposure to sulforaphane-rich BSE. Together, our findings demonstrate preclinical chemopreventive activity of sulforaphane against carcinogen-induced oral cancer, and support further mechanistic and clinical investigation of sulforaphane as a chemopreventive agent against tobacco-related HNSCC. Cancer Prev Res; 9(7); 547–57. ©2016 AACR.
Circulating Osteopontin and Prediction of Hepatocellular Carcinoma Development in a Large European Population
Authors: Duarte-Salles Talita , Misra Sandeep , Stepien Magdalena , Plymoth Amelie , Muller David , Overvad Kim , Olsen Anja , Tjønneland Anne , Baglietto Laura , Severi Gianluca , Boutron-Ruault Marie-Christine , Turzanski-Fortner Renee , Kaaks Rudolf , Boeing Heiner , Aleksandrova Krasimira , Trichopoulou Antonia , Lagiou Pagona , Bamia Christina , Pala Valeria , Palli Domenico , Mattiello Amalia , Tumino Rosario , Naccarati Alessio , Bueno-de-Mesquita H.B(as). , Peeters Petra H. , Weiderpass Elisabete , Quirós J. Ramón , Agudo Antonio , Sánchez-Cantalejo Emilio , Ardanaz Eva , Gavrila Diana , Dorronsoro Miren , Werner Mårten , Hemmingsson Oskar , Ohlsson Bodil , Sjöberg Klas , Wareham Nicholas J. , Khaw Kay-Tee , Bradbury Kathryn E. , Gunter Marc J. , Cross Amanda J. , Riboli Elio , Jenab Mazda , Hainaut Pierre , Beretta Laura 
Published: June 2016

Abstract
We previously identified osteopontin (OPN) as a promising marker for the early detection of hepatocellular carcinoma (HCC). In this study, we investigated the association between prediagnostic circulating OPN levels and HCC incidence in a large population-based cohort. A nested case–control study was conducted within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. During a mean follow-up of 4.8 years, 100 HCC cases were identified. Each case was matched to two controls and OPN levels were measured in baseline plasma samples. Viral hepatitis, liver function, and α-fetoprotein (AFP) tests were also conducted. Conditional logistic regression models were used to calculate multivariable odds ratio (OR) and 95% confidence intervals (95% CI) for OPN levels in relation to HCC. Receiver operating characteristics curves were constructed to determine the discriminatory accuracy of OPN alone or in combination with other liver biomarkers in the prediction of HCC. OPN levels were positively associated with HCC risk (per 10% increment, ORmultivariable = 1.30; 95% CI, 1.14–1.48). The association was stronger among cases diagnosed within 2 years of follow-up. Adding liver function tests to OPN improved the discriminatory performance for subjects who developed HCC (AUC = 0.86). For cases diagnosed within 2 years, the combination of OPN and AFP was best able to predict HCC risk (AUC = 0.88). The best predictive model for HCC in this low-risk population is OPN in combination with liver function tests. Within 2 years of diagnosis, the combination of OPN and AFP best predicted HCC development, suggesting that measuring OPN and AFP could identify high-risk groups independently of a liver disease diagnosis. Cancer Prev Res; 9(9); 758–65. ©2016 AACR.
Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma
Authors: Wang Feng , Huang Jian , Zhu Zhu , Ma Xiao , Cao Li , Zhang Yongzhi , Chen Wei , Dong Yang 
Published: June 2016

Abstract
Early detection of hepatocellular carcinoma (HCC) is critical for successful treatment and favorable prognosis. To identify novel HCC biomarkers, we used the WHV/c-myc transgenic (Tg) mice, an animal model of hepatocarcinogenesis. By analyzing their gene expression profiling, we investigated differentially expressed genes in livers of wild-type and Tg mice. The cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), a hepatic P450 enzyme, was revealed to be overexpressed in the liver tissues of Tg mice at both preneoplastic and neoplastic stages. Mouse-to-human validation demonstrated that CYP17A1 mRNA and protein were also significantly increased in human HCC tissues compared with paired nontumor tissues (P = 0.00041 and 0.00011, respectively). Immunohistochemical studies showed that CYP17A1 was overexpressed in 67% (58 of 87) of HCC, and strong staining of CYP17A1 was observed in well-differentiated HCCs. Consistent with this, the median serum levels of CYP17A1 were also significantly higher in patients with HCC (140.2 ng/mL, n = 776) compared with healthy controls (31.4 ng/mL, n = 366) and to those with hepatitis B virus (57.5 ng/mL, n = 160), cirrhosis (46.1 ng/mL, n = 147), lung cancer (27.4 ng/mL, n = 109), and prostate cancer (42.1 ng/mL, n = 130; all P &lt; 0.001). Notably, the elevations were seen in most AFP-negative HCC cases. Altogether, through mouse-to-human search and validation, we found that CYP17A1 is overexpressed in HCCs and it has great potentiality as a noninvasive marker for HCC detection. These results provide a rationale for the future development and clinical application of CYP17A1 measurement to diagnose HCC more precisely. Cancer Prev Res; 9(9); 739–49. ©2016 AACR.