bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological and health‐related response of broiler chickens fed diets containing raw, full‐fat soya bean meal supplemented with microbial protease
Authors: Erdaw M. M. , Perez‐Maldonado R. A. , Iji P. A. 
Published: October 2017

SummaryA 2 × 3 factorial study (protease: 0 or 1,5000 PROT/kg and raw full‐fat soya bean meal [RSBM] replacing the commercial SBM at 0, 45 and 75 g/kg of diet) was conducted to examine the performance of broilers. Phytase (2000 FYT/kg) was uniformly added to each diet, each also replicated six times, with eight birds per replicate. Birds were raised in climate‐controlled rooms using sawdust as the bedding material and offered starter, grower and finisher diets. Feed intake (FI) and body weight gain (BWG) were reduced (p < .05) due to increasing levels of RSBM, but feed conversion ratio (FCR; 0–35 days) was unaffected. Over the first 24 days, neither RSBM nor protease supplementation affected (p > .05) mortality, footpad dermatitis or intestinal lesions in birds. At day 24, the weight, length, width and strength of tibia bone were reduced in chickens that received an elevated level of RSBM (75 g/kg of diet), but this was not significant at day 35. At day 24 (p < .05) and 35 (p < .01), Ca concentration in the litter was reduced when the RSBM level was increased in the diet, but P content was not affected. On days 24 (p < .05) and 35 (p < .01), the N content in litter was also increased with increase in dietary RSBM. Protease supplementation increased (p < .05) the uric acid concentration in the litter (at day 35), but the reverse was the case for ammonia concentration. Overall, the results of this study indicate that there are no major health‐related risks, associated with the replacement of commercial SBM with RSBM (≤25%) in broiler diets.
Sleep duration is associated with sperm chromatin integrity among young men in Chongqing, China
Authors: Wang Xiaogang , Chen Qing , Zou Peng , Liu Taixiu , Mo Min , Yang Huan , Zhou Niya , Sun Lei , Chen Hongqiang , Ling Xi , Peng Kaige , Ao Lin , Yang Huifang , Cao Jia , Cui Zhihong 
Published: October 2017

SummaryThis study explores whether sleep duration is associated with sperm chromatin integrity. To do so, we conducted a three‐phase panel study of 796 male volunteers from colleges in Chongqing (China) from 2013 to 2015. Sleep duration was measured using a modified Munich Chronotype Questionnaire. Sperm DNA integrity was examined via Sperm Chromatin Structure Assay and Comet assay. Setting 7–7.5 h day−1 of sleep duration as a reference, either longer or shorter sleep duration was associated negatively with high DNA stainability (HDS) (P = 0.009), which reflected the immaturity of sperm chromatin. The volunteers with > 9.0 h day−1 sleep and those with ≤ 6.5 h day−1 sleep had 40.7 and 30.3% lower HDS than did volunteers with 7–7.5 h day−1 sleep. No association was found between sleep duration and DNA fragmentation index or Comet assay parameters. This study suggests that sleep duration is associated with sperm chromatin integrity. Further studies are required to validate these findings and investigate the mechanism underlying this association.
Genomics of adaptive divergence with chromosome‐scale heterogeneity in crossover rate
Authors: Berner Daniel , Roesti Marius 
Published: October 2017

AbstractGenetic differentiation between divergent populations is often greater in chromosome centres than peripheries. Commonly overlooked, this broadscale differentiation pattern is sometimes ascribed to heterogeneity in crossover rate and hence linked selection within chromosomes, but the underlying mechanisms remain incompletely understood. A literature survey across 46 organisms reveals that most eukaryotes indeed exhibit a reduced crossover rate in chromosome centres relative to the peripheries. Using simulations of populations diverging into ecologically different habitats through sorting of standing genetic variation, we demonstrate that such chromosome‐scale heterogeneity in crossover rate, combined with polygenic divergent selection, causes stronger hitchhiking and especially barriers to gene flow across chromosome centres. Without requiring selection on new mutations, this rapidly leads to elevated population differentiation in the low‐crossover centres relative to the high‐crossover peripheries of chromosomes (“Chromosome Centre‐Biased Differentiation”, CCBD). Using simulated and empirical data, we then show that strong CCBD between populations can provide evidence of polygenic adaptive divergence with a phase of gene flow. We further demonstrate that chromosome‐scale heterogeneity in crossover rate impacts analyses beyond that of population differentiation, including the inference of phylogenies and parallel adaptive evolution among populations, the detection of genetic loci under selection, and the interpretation of the strength of selection on genomic regions. Overall, our results call for a greater appreciation of chromosome‐scale heterogeneity in crossover rate in evolutionary genomics.
Beyond Realism and Moralism: A Defense of Political Minimalism
Authors: Rodríguez‐Alcázar Javier 
Published: October 2017

AbstractWhat is the relationship between morals and politics? What is the relationship between moral philosophy and political philosophy? Defenders of political moralism postulate moral aims or constraints for politics, and hence they see political philosophy as a chapter of moral philosophy. Contrastingly, advocates of political realism describe politics as an independent endeavor aiming at providing order and security, and conceive of political philosophy as an autonomous discipline. This article claims that political moralism and political realism share the mistake of assuming that politics has substantial, permanent goals or constraints. After criticizing political substantialism, the article explains the main ingredients of its alternative, political minimalism: (1) the idea that politics, understood as collective instrumental rationality, aims at providing adequate means for the accomplishment of people's goals, whatever these are; and (2) the conception of the relationship between morality and politics as one of “reciprocal containment.” Finally, it addresses some foreseeable criticisms of political minimalism.
Toward a Philosophy of Blockchain: A Symposium: Introduction
Authors: Swan Melanie , de Filippi Primavera 
Published: October 2017

AbstractThis article introduces the symposium “Toward a Philosophy of Blockchain,” which provides a philosophical contemplation of blockchain technology, the digital ledger software underlying cryptocurrencies such as bitcoin, for the secure transfer of money, assets, and information via the Internet without needing a third‐party intermediary. The symposium offers philosophical scholarship on a new topic, blockchain technology, from a variety of perspectives. The philosophical themes discussed include mathematical models of reality, signification, and the sociopolitical institutions that structure human life and interaction. The symposium also investigates the metaphilosophical theme of how to create a philosophy of anything, specifically a new topic such as blockchain technology. Repeated themes are identified, in all areas of philosophical inquiry (ontology, epistemology, and axiology), and conceptual resources are elaborated to contribute to a philosophical understanding of blockchain technology. Thus, philosophy as a metaphilosophical approach is shown to be able to provide an understanding of the conceptual, theoretical, and foundational dimensions of novelty and emergence in the world, with a particular focus on blockchain technology.
On the Continuity and Origin of Identity in Distributed Ledgers: Learning from Russell's Paradox
Authors: Parra Moyano JosÉ 
Published: October 2017

AbstractThis article studies the origin and continuity of the identity of the entities inscribed in a distributed ledger. Specifically, it focuses on the differences between the identities of the entities that exist in a distributed ledger and those of the entities that exist outside the ledger but must be represented in the ledger in order to interact with it. It suggests that a distributed ledger that contains representations of entities that exist outside the ledger can yield a continuum of interconnected existing and past identities that is constantly redefined to represent new conceptual entities. This continuum can be understood as a metasortal—or a sortal of sortals—that resembles the mathematical structure of a set of sets. Further, the article presents the dilemma that arises when representing the identities of entities in a distributed ledger, and it draws an analogy between this dilemma and Russell's Paradox.
The Metaphors of Knowledge and Academic Impact
Authors: Kivistö Sari , Pihlström Sami 
Published: October 2017

AbstractThis essay discusses critically the ways in which different metaphors employed to illustrate the practices of knowledge production and knowledge acquisition as well as scientific and scholarly research shape our understanding of the academic form of life. The essay examines the metaphors of knowledge and their role in academia by means of philosophical analysis and a rhetorical analysis of language, thereby defending the core values of academic freedom. It focuses on two pairs of metaphors highly relevant to the tensions characteristic of contemporary academic work: verticality and horizontality, on the one hand, and change and stability, on the other. The entanglement of perspectives from philosophy and historical rhetoric serves a metaphilosophical goal here: to show that our philosophical understanding of the nature of inquiry will be considerably enhanced if we seriously, and with both historical and literary sensibilities, study the metaphors we use in characterizing our epistemic and cognitive projects.
Computing Ledgers and the Political Ontology of the Blockchain
Authors: Velasco Pablo R. 
Published: October 2017

AbstractThis paper investigates ontological dimensions of the blockchain by asking what kind of socio‐technical object bitcoin is. It discusses both blockchain's political qualities and the political forms enabled by its emergence. It first observes recent approaches to the ontology of money and the political qualities of the ledgers used by the current fractional reserve banking model. It then directs the same questions at blockchain technology. The paper discusses an ontology proposed by Ole Bjerg (2016) and argues in favour of a mixed‐ontology approach to blockchains. It then questions the political qualities of the distributed ledger as a digital object and highlights the apparent absence of authority figures in the model. Finally, it argues that the political ontology of the blockchain can be framed as the displacement of authority from institutional actors into instrumental control of trust, in a dynamically distributed environment.
Multiple Taf subunits of TFIID interact with Ino2 activation domains and contribute to expression of genes required for yeast phospholipid biosynthesis
Authors: Hintze Stefan , Engelhardt Maike , van Diepen Laura , Witt Eric , Schüller Hans‐Joachim 
Published: October 2017

SummaryExpression of phospholipid biosynthetic genes in yeast requires activator protein Ino2 which can bind to the UAS element inositol/choline‐responsive element (ICRE) and trigger activation of target genes, using two separate transcriptional activation domains, TAD1 and TAD2. However, it is still unknown which cofactors mediate activation by TADs of Ino2. Here, we show that multiple subunits of basal transcription factor TFIID (TBP‐associated factors Taf1, Taf4, Taf6, Taf10 and Taf12) are able to interact in vitro with activation domains of Ino2. Interaction was no longer observed with activation‐defective variants of TAD1. We were able to identify two nonoverlapping regions in the N‐terminus of Taf1 (aa 1–100 and aa 182–250) each of which could interact with TAD1 of Ino2 as well as with TAD4 of activator Adr1. Specific missense mutations within Taf1 domain aa 182–250 affecting basic and hydrophobic residues prevented interaction with wild‐type TAD1 and caused reduced expression of INO1. Using chromatin immunoprecipitation we demonstrated Ino2‐dependent recruitment of Taf1 and Taf6 to ICRE‐containing promoters INO1 and CHO2. Transcriptional derepression of INO1 was no longer possible with temperature‐sensitive taf1 and taf6 mutants cultivated under nonpermissive conditions. This result supports the hypothesis of Taf‐dependent expression of structural genes activated by Ino2.
Developmental stage influences chromosome segregation patterns and arrangement in the extremely polyploid, giant bacterium Epulopiscium sp. type B
Authors: Hutchison Elizabeth , Yager Nicholas A. , Taw May N. , Taylor Matthew , Arroyo Francine , Sannino David R. , Angert Esther R. 
Published: October 2017

SummaryFew studies have described chromosomal dynamics in bacterial cells with more than two complete chromosome copies or described changes with respect to development in polyploid cells. We examined the arrangement of chromosomal loci in the very large, highly polyploid, uncultivated intestinal symbiont Epulopiscium sp. type B using fluorescent in situ hybridization. We found that in new offspring, chromosome replication origins (oriCs) are arranged in a three‐dimensional array throughout the cytoplasm. As development progresses, most oriCs become peripherally located. Siblings within a mother cell have similar numbers of oriCs. When chromosome orientation was assessed in situ by labeling two chromosomal regions, no specific pattern was detected. The Epulopiscium genome codes for many of the conserved positional guide proteins used for chromosome segregation in bacteria. Based on this study, we present a model that conserved chromosomal maintenance proteins, combined with entropic demixing, provide the forces necessary for distributing oriCs. Without the positional regulation afforded by radial confinement, chromosomes are more randomly oriented in Epulopiscium than in most small rod‐shaped cells. Furthermore, we suggest that the random orientation of individual chromosomes in large polyploid cells would not hamper reproductive success as it would in smaller cells with more limited genomic resources.
X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants
Authors: van der Ent Antony , Przybyłowicz Wojciech J. , de Jonge Martin D. , Harris Hugh H. , Ryan Chris G. , Tylko Grzegorz , Paterson David J. , Barnabas Alban D. , Kopittke Peter M. , Mesjasz‐Przybyłowicz Jolanta 
Published: October 2017

Contents

Summary
432

I.
Introduction
433

II.
Preparation of plant samples for X‐ray micro‐analysis
433

III.
X‐ray elemental mapping techniques
438

IV.
X‐ray data analysis
442

V.
Case studies
443

VI.
Conclusions
446

Acknowledgements
449

Author contributions
449

References
449

SummaryHyperaccumulators are attractive models for studying metal(loid) homeostasis, and probing the spatial distribution and coordination chemistry of metal(loid)s in their tissues is important for advancing our understanding of their ecophysiology. X‐ray elemental mapping techniques are unique in providing in situ information, and with appropriate sample preparation offer results true to biological conditions of the living plant. The common platform of these techniques is a reliance on characteristic X‐rays of elements present in a sample, excited either by electrons (scanning/transmission electron microscopy), protons (proton‐induced X‐ray emission) or X‐rays (X‐ray fluorescence microscopy). Elucidating the cellular and tissue‐level distribution of metal(loid)s is inherently challenging and accurate X‐ray analysis places strict demands on sample collection, preparation and analytical conditions, to avoid elemental redistribution, chemical modification or ultrastructural alterations. We compare the merits and limitations of the individual techniques, and focus on the optimal field of applications for inferring ecophysiological processes in hyperaccumulator plants. X‐ray elemental mapping techniques can play a key role in answering questions at every level of metal(loid) homeostasis in plants, from the rhizosphere interface, to uptake pathways in the roots and shoots. Further improvements in technological capabilities offer exciting perspectives for the study of hyperaccumulator plants into the future.
Addressing weight bias and discrimination: moving beyond raising awareness to creating change
Authors: Ramos Salas X. , Alberga A. S. , Cameron E. , Estey L. , Forhan M. , Kirk S. F. L. , Russell‐Mayhew S. , Sharma A. M. 
Published: October 2017

SummaryWeight discrimination is the unjust treatment of individuals because of their weight. There have been very few interventions to address weight discrimination, due in part to the lack of consensus on key messages and strategies. The objective of the third Canadian Weight Bias Summit was to review current evidence and move towards consensus on key weight bias and obesity discrimination reduction messages and strategies. Using a modified brokered dialogue approach, participants, including researchers, health professionals, policy makers and people living with obesity, reviewed the evidence and moved towards consensus on key messages and strategies for future interventions. Participants agreed to these key messages: (1) Weight bias and obesity discrimination should not be tolerated in education, health care and public policy sectors; (2) obesity should be recognized and treated as a chronic disease in health care and policy sectors; and (3) in the education sector, weight and health need to be decoupled. Consensus on future strategies included (1) creating resources to support policy makers, (2) using personal narratives from people living with obesity to engage audiences and communicate anti‐discrimination messages and (3) developing a better clinical definition for obesity. Messages and strategies should be implemented and evaluated using consistent theoretical frameworks and methodologies.
Bacteremia after supragingival scaling and dental extraction: Culture and molecular analyses
Authors: Reis LC , Rôças IN , Siqueira JF , de Uzeda M , Lacerda VS , Domingues RMCP , Miranda KR , Saraiva RM 
Published: October 2017

ObjectiveTo study the incidence and magnitude of bacteremia after dental extraction and supragingival scaling.Subjects and methodsBlood samples were taken before and 5 and 30 min after dental extraction and supragingival scaling from individuals at high (n = 44) or negligible risk (n = 51) for infective endocarditis. The former received prophylactic antibiotic therapy. Samples were subjected to aerobic and anaerobic culture and quantitative real‐time polymerase chain reaction to determine the incidence of bacteremia and total bacterial levels.ResultsPatients who did not receive prophylactic antibiotic therapy had a higher incidence of positive blood cultures (30% 5 min after extraction) than patients who received prophylactic antibiotic therapy (0% 5 min after extraction; p < .01). Molecular analysis did not reveal significant differences in the incidence or magnitude of bacteremia between the two patient groups either 5 or 30 min after each of the procedures evaluated. Extraction was associated with higher incidence of bacteremia than supragingival scaling by blood culture (p = .03) and molecular analysis (p = .05).ConclusionsMolecular methods revealed that dental extraction and supragingival scaling were associated with similar incidence of bacteremia in groups receiving or not prophylactic antibiotic therapy. However, blood culture revealed that antibiotic therapy reduced viable cultivable bacteria in the bloodstream in the extraction group.
Use of immature seed germination and single seed descent for rapid genetic gains in pigeonpea
Authors: Saxena Kulbhushan , Saxena Rachit K. , Varshney Rajeev K. 
Published: October 2017

AbstractLong duration required for generation advancement in pigeonpea [Cajanus cajan (L.) Millsp] is one of the major bottlenecks in realizing rapid genetic gains. Therefore, a technology for rapid generation turnover is warranted to facilitate the development of new cultivars and recombinant inbred lines. Breeding of early‐maturing cultivars has now opened up the possibility of rapid generation advance (RGA) in this crop. This paper reports the development of an RGA technology that integrates the germination of immature seeds with single seed descent method of breeding. The results showed that immature 35‐day‐old seeds can be used successfully to turn over a generation of pigeonpea with 100% seed germination. These way 3/4 successive generations can be grown within a year. The methodology presented in this study will accelerate the breeding process for breeding cultivars and develop rapidly the materials required for genomics research in pigeonpea.
Accountability and Coalitions: Evidence from a Negotiation Experiment
Authors: Bouwman Robin , van Thiel Sandra , van Deemen Ad , Rouwette Etiënne 
Published: October 2017

AbstractThis article tests the effect of accountability on negotiation outcomes in a face‐to‐face classroom experiment. Student participants were asked to form coalitions in groups of three. In the treatment condition, negotiators were held accountable by a personal forum during the formation of the coalition. In the control condition, negotiators were not held accountable. Results show that accountability leads to lower group performance in coalition negotiations. Accountability also reduced the willingness of negotiators to include all negotiators in a “grand coalition.” Rather, accountable negotiators reached agreement with a subset of negotiators. Accountability increased the odds of reaching no agreement. These findings challenge the idea of increased performance as a result of public accountability in the context of coalition negotiations.
Utilization of increased risk for transmission of infectious disease donor organs in solid organ transplantation: Retrospective analysis of disease transmission and safety
Authors: Irwin Linda , Kotton Camille N. , Elias Nahel , Palafox Julie , Basler Debra , Shao Sarah H. , Lester William , Zhang Xiaofeng , Kimball Brendan , Trencher Carrie , Fishman Jay A. 
Published: October 2017

AbstractThe inadequate supply of transplantable organs necessitates new approaches to organ availability. Serologies and nucleic acid testing (NAT) for hepatitis C virus (HCV), hepatitis B virus (HBV), and human immunodeficiency virus (HIV) are used in microbiologic screening of potential organ donors. Organs from donors considered at “high risk” (Centers for Disease Control and Prevention, CDC 1994) or “increased risk” (U.S. Public Health Service, PHS 2013) for transmission of viral infection to recipients may provide an expanded source of organs for transplantation. We review a single‐center experience with 257 adult organ recipients of organs from donors meeting either CDC 1994 or PHS 2013 risk criteria between 2011 and 2016. Tracking these transplants required modification of the Transplant Center electronic database to identify all recipients of increased‐risk donor (IRD) organs, documentation of informed consent, and microbiologic testing data. No transmissions of HIV, HBV, or HCV were identified by NAT or clinically. Nine patients developed positive serologic assays for one of the tested viruses; all recipients were retested and remain negative by NAT. Notably, post‐transplant HBV core serologies reverted to negative on re‐testing; these positive serologies are likely false positives caused by receipt of blood products. Use of IRD organs can be performed safely with appropriate informed consent and rigorous pre‐ and post‐transplant microbiological testing.