bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of climate change on photovoltaic power generation in Europe
Authors: Jerez Sonia , Tobin Isabelle , Vautard Robert , Montávez Juan Pedro , López-Romero Jose María , Thais Françoise , Bartok Blanka , Christensen Ole Bøssing , Colette Augustin , Déqué Michel , Nikulin Grigory , Kotlarski Sven , van Meijgaard Erik , Teichmann Claas , Wild Martin 
Published: December 2015

AbstractAmbitious climate change mitigation plans call for a significant increase in the use of renewables, which could, however, make the supply system more vulnerable to climate variability and changes. Here we evaluate climate change impacts on solar photovoltaic (PV) power in Europe using the recent EURO-CORDEX ensemble of high-resolution climate projections together with a PV power production model and assuming a well-developed European PV power fleet. Results indicate that the alteration of solar PV supply by the end of this century compared with the estimations made under current climate conditions should be in the range (−14%;+2%), with the largest decreases in Northern countries. Temporal stability of power generation does not appear as strongly affected in future climate scenarios either, even showing a slight positive trend in Southern countries. Therefore, despite small decreases in production expected in some parts of Europe, climate change is unlikely to threaten the European PV sector.
Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage
Authors: Dvash Efrat , Har-Tal Michal , Barak Sara , Meir Ofir , Rubinstein Menachem 
Published: December 2015

AbstractEndoplasmic reticulum (ER) stress and major chemotherapeutic agents damage DNA by generating reactive oxygen species (ROS). Here we show that ER stress and chemotherapy induce leukotriene C4 (LTC4) biosynthesis by transcriptionally upregulating and activating the enzyme microsomal glutathione-S-transferase 2 (MGST2) in cells of non-haematopoietic lineage. ER stress and chemotherapy also trigger nuclear translocation of the two LTC4 receptors. Acting in an intracrine manner, LTC4 then elicits nuclear translocation of NADPH oxidase 4 (NOX4), ROS accumulation and oxidative DNA damage. Mgst2 deficiency, RNAi and LTC4 receptor antagonists abolish ER stress- and chemotherapy-induced ROS and oxidative DNA damage in vitro and in mouse kidneys. Cell death and mouse morbidity are also significantly attenuated. Hence, MGST2-generated LTC4 is a major mediator of ER stress- and chemotherapy-triggered oxidative stress and oxidative DNA damage. LTC4 inhibitors, commonly used for asthma, could find broad clinical use in major human pathologies associated with ER stress-activated NOX4.
Structure of a bacterial type III secretion system in contact with a host membrane in situ
Authors: Nans Andrea , Kudryashev Mikhail , Saibil Helen R. , Hayward Richard D. 
Published: December 2015

AbstractMany bacterial pathogens of animals and plants use a conserved type III secretion system (T3SS) to inject virulence effector proteins directly into eukaryotic cells to subvert host functions. Contact with host membranes is critical for T3SS activation, yet little is known about T3SS architecture in this state or the conformational changes that drive effector translocation. Here we use cryo-electron tomography and sub-tomogram averaging to derive the intact structure of the primordial Chlamydia trachomatis T3SS in the presence and absence of host membrane contact. Comparison of the averaged structures demonstrates a marked compaction of the basal body (4 nm) occurs when the needle tip contacts the host cell membrane. This compaction is coupled to a stabilization of the cytosolic sorting platform–ATPase. Our findings reveal the first structure of a bacterial T3SS from a major human pathogen engaged with a eukaryotic host, and reveal striking ‘pump-action’ conformational changes that underpin effector injection.
The genetic basis of natural variation in mushroom body size in Drosophila melanogaster
Authors: Zwarts Liesbeth , Vanden Broeck Lies , Cappuyns Elisa , Ayroles Julien F. , Magwire Michael M. , Vulsteke Veerle , Clements Jason , Mackay Trudy F. C. , Callaerts Patrick 
Published: December 2015

AbstractGenetic variation in brain size may provide the basis for the evolution of the brain and complex behaviours. The genetic substrate and the selective pressures acting on brain size are poorly understood. Here we use the Drosophila Genetic Reference Panel to map polymorphic variants affecting natural variation in mushroom body morphology. We identify 139 genes and 39 transcription factors and confirm effects on development and adult plasticity. We show correlations between morphology and aggression, sleep and lifespan. We propose that natural variation in adult brain size is controlled by interaction of the environment with gene networks controlling development and plasticity.
Association between cadmium exposure and renal cancer risk: a meta-analysis of observational studies
Authors: Song Ju kun , Luo Hong , Yin Xin hai , Huang Guang lei , Luo Si yang , Lin Du ren , Yuan Dong Bo , Zhang Wei , Zhu Jian guo 
Published: December 2015

AbstractCadmium (Cd) is a widespread environmental pollutant and has been a recognized carcinogen for several decades. Many observational studies reported Cd exposure might be one cause of renal cancer. However, these findings are inconsistent. We conducted a meta-analysis to evaluate the relationship between cadmium exposure and renal cancer risk. A comprehensive PubMed and Embase search was conducted to retrieve observational studies meeting our meta-analysis criteria. A combined odds ratio (OR) and corresponding 95% confidence interval (CI) were applied to assess the association between Cd exposure and renal cancer risk. The meta-analysis showed that a high Cd exposure significantly increased renal cancer 1.47 times (OR = 1.47; 95% CI = 1.27 to 1.71, for highest versus lowest category of cadmium categories). The significant association remained consistent when stratified by geographic region and gender, however mixed results were produced when stratified by sample size, study design, NOS score, adjustment for covariates, effects measure and exposure type. Our results indicated that a high Cd exposure was associated with increased renal cancer risk and the association was higher for occupational exposure compared with non-occupational exposure. This meta-analysis suggests that a high Cd exposure may be a risk factor for renal cancer in occupational population.
Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance
Authors: Hsu Alan Yi-Hui , Wu Shang-Rung , Tsai Jih-Jin , Chen Po-Lin , Chen Ya-Ping , Chen Tsai-Yun , Lo Yu-Chih , Ho Tzu-Chuan , Lee Meed , Chen Min-Ting , Chiu Yen-Chi , Perng Guey Chuen 
Published: December 2015

AbstractThe levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a “sunny-side up egg” appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.
Negative differential resistance and characteristic nonlinear electromagnetic response of a Topological Insulator
Authors: Lee Ching Hua , Zhang Xiao , Guan Bochen 
Published: December 2015

AbstractMaterials exhibiting negative differential resistance have important applications in technologies involving microwave generation, which range from motion sensing to radio astronomy. Despite their usefulness, there has been few physical mechanisms giving rise to materials with such properties, i.e. GaAs employed in the Gunn diode. In this work, we show that negative differential resistance also generically arise in Dirac ring systems, an example of which has been experimentally observed in the surface states of Topological Insulators. This novel realization of negative differential resistance is based on a completely different physical mechanism from that of the Gunn effect, relying on the characteristic non-monotonicity of the response curve that remains robust in the presence of nonzero temperature, chemical potential, mass gap and impurity scattering. As such, it opens up new possibilities for engineering applications, such as frequency upconversion devices which are highly sought for terahertz signal generation. Our results may be tested with thin films of Bi2Se3 Topological Insulators and are expected to hold qualitatively even in the absence of a strictly linear Dirac dispersion, as will be the case in more generic samples of Bi2Se3 and other materials with topologically nontrivial Fermi sea regions.
Global land moisture trends: drier in dry and wetter in wet over land
Authors: Feng Huihui , Zhang Mingyang 
Published: December 2015

AbstractThe “dry gets drier, wet gets wetter” (DGDWGW) paradigm is widely accepted in global moisture change. However, Greve et al.1 have declared that this paradigm has been overestimated. This controversy leaves a large gap in the understanding of the evolution of water-related processes. Here, we examine the global moisture trends using satellite soil moisture for the past 35 years (1979–2013). Our results support those of Greve et al., although there are quantitative differences. Generally, approximately 30% of global land has experienced robust moisture trends (22.16% have become drier and 7.14% have become wetter). Only 15.12% of the land areas have followed the DGDWGW paradigm, whereas 7.77% have experienced the opposite trend. A new finding is that there is a significant “drier in dry, wetter in wet” (DIDWIW) trend paradigm; 52.69% of the drying trend occurred in arid regions and 48.34% of the wetter trend occurred in the humid regions. Overall, 51.63% of the trends followed the DIDWIW paradigm and 26.93% followed the opposite trend. We also identified the DGDWGW and DIDWIW paradigms in low precipitation-induced arid regions in which the dry soil led to an increasing sensible heat flux and temperature and subsequently potential evapotranspiration.
Improvement of genome assembly completeness and identification of novel full-length protein-coding genes by RNA-seq in the giant panda genome
Authors: Chen Meili , Hu Yibo , Liu Jingxing , Wu Qi , Zhang Chenglin , Yu Jun , Xiao Jingfa , Wei Fuwen , Wu Jiayan 
Published: December 2015

AbstractHigh-quality and complete gene models are the basis of whole genome analyses. The giant panda (Ailuropoda melanoleuca) genome was the first genome sequenced on the basis of solely short reads, but the genome annotation had lacked the support of transcriptomic evidence. In this study, we applied RNA-seq to globally improve the genome assembly completeness and to detect novel expressed transcripts in 12 tissues from giant pandas, by using a transcriptome reconstruction strategy that combined reference-based and de novo methods. Several aspects of genome assembly completeness in the transcribed regions were effectively improved by the de novo assembled transcripts, including genome scaffolding, the detection of small-size assembly errors, the extension of scaffold/contig boundaries and gap closure. Through expression and homology validation, we detected three groups of novel full-length protein-coding genes. A total of 12.62% of the novel protein-coding genes were validated by proteomic data. GO annotation analysis showed that some of the novel protein-coding genes were involved in pigmentation, anatomical structure formation and reproduction, which might be related to the development and evolution of the black-white pelage, pseudo-thumb and delayed embryonic implantation of giant pandas. The updated genome annotation will help further giant panda studies from both structural and functional perspectives.
A Mechanistic Understanding of a Binary Additive System to Synergistically Boost Efficiency in All-Polymer Solar Cells
Authors: Kim Yu Jin , Ahn Sunyong , Wang Dong Hwan , Park Chan Eon 
Published: December 2015

AbstractAll-polymer solar cells are herein presented utilizing the PBDTTT-CT donor and the P(NDI2OD-T2) acceptor with 1,8-diiodooctane (DIO) and 1-chloronaphthalene (CN) binary solvent additives. A systematic study of the polymer/polymer bulk heterojunction photovoltaic cells processed from the binary additives revealed that the microstructures and photophysics were quite different from those of a pristine system. The combination of DIO and CN with a DIO/CN ratio of 3:1 (3 vol% DIO, 1 vol% CN and 96 vol% o-DCB) led to suitable penetrating polymer networks, efficient charge generation and balanced charge transport, which were all beneficial to improving the efficiency. This improvement is attributed to increase in power conversion efficiency from 2.81% for a device without additives to 4.39% for a device with the binary processing additives. A detailed investigation indicates that the changes in the polymer:polymer interactions resulted in the formation of a percolating nasnoscale morphology upon processing with the binary additives. Depth profile measurements with a two-dimensional grazing incidence wide-angle X-ray scattering confirm this optimum phase feature. Furthermore impedance spectroscopy also finds evidence for synergistically boosting the device performance.
Mitotic Spindle Disruption by Alternating Electric Fields Leads to Improper Chromosome Segregation and Mitotic Catastrophe in Cancer Cells
Authors: Giladi Moshe , Schneiderman Rosa S , Voloshin Tali , Porat Yaara , Munster Mijal , Blat Roni , Sherbo Shay , Bomzon Zeev , Urman Noa , Itzhaki Aviran , Cahal Shay , Shteingauz Anna , Chaudhry Aafia , Kirson Eilon D , Weinberg Uri , Palti Yoram 
Published: December 2015

AbstractTumor Treating Fields (TTFields) are low intensity, intermediate frequency, alternating electric fields. TTFields are a unique anti-mitotic treatment modality delivered in a continuous, noninvasive manner to the region of a tumor. It was previously postulated that by exerting directional forces on highly polar intracellular elements during mitosis, TTFields could disrupt the normal assembly of spindle microtubules. However there is limited evidence directly linking TTFields to an effect on microtubules. Here we report that TTFields decrease the ratio between polymerized and total tubulin and prevent proper mitotic spindle assembly. The aberrant mitotic events induced by TTFields lead to abnormal chromosome segregation, cellular multinucleation and caspase dependent apoptosis of daughter cells. The effect of TTFields on cell viability and clonogenic survival substantially depends upon the cell division rate. We show that by extending the duration of exposure to TTFields, slowly dividing cells can be affected to a similar extent as rapidly dividing cells.
Environmental transmission of Mycobacterium ulcerans drives dynamics of Buruli ulcer in endemic regions of Cameroon
Authors: Garchitorena Andrés , Ngonghala Calistus N. , Texier Gaëtan , Landier Jordi , Eyangoh Sara , Bonds Matthew H. , Guégan Jean-François , Roche Benjamin 
Published: December 2015

AbstractBuruli Ulcer is a devastating skin disease caused by the pathogen Mycobacterium ulcerans. Emergence and distribution of Buruli ulcer cases is clearly linked to aquatic ecosystems, but the specific route of transmission of M. ulcerans to humans remains unclear. Relying on the most detailed field data in space and time on M. ulcerans and Buruli ulcer available today, we assess the relative contribution of two potential transmission routes –environmental and water bug transmission– to the dynamics of Buruli ulcer in two endemic regions of Cameroon. The temporal dynamics of Buruli ulcer incidence are explained by estimating rates of different routes of transmission in mathematical models. Independently, we also estimate statistical models of the different transmission pathways on the spatial distribution of Buruli ulcer. The results of these two independent approaches are corroborative and suggest that environmental transmission pathways explain the temporal and spatial patterns of Buruli ulcer in our endemic areas better than the water bug transmission.
Time-resolved observation of band-gap shrinking and electron-lattice thermalization within X-ray excited gallium arsenide
Authors: Ziaja Beata , Medvedev Nikita , Tkachenko Victor , Maltezopoulos Theophilos , Wurth Wilfried 
Published: December 2015

AbstractFemtosecond X-ray irradiation of solids excites energetic photoelectrons that thermalize on a timescale of a few hundred femtoseconds. The thermalized electrons exchange energy with the lattice and heat it up. Experiments with X-ray free-electron lasers have unveiled so far the details of the electronic thermalization. In this work we show that the data on transient optical reflectivity measured in GaAs irradiated with femtosecond X-ray pulses can be used to follow electron-lattice relaxation up to a few tens of picoseconds. With a dedicated theoretical framework, we explain the so far unexplained reflectivity overshooting as a result of band-gap shrinking. We also obtain predictions for a timescale of electron-lattice thermalization, initiated by conduction band electrons in the temperature regime of a few eVs. The conduction and valence band carriers were then strongly non-isothermal. The presented scheme is of general applicability and can stimulate further studies of relaxation within X-ray excited narrow band-gap semiconductors.
Enhanced performance of an innovative dengue IgG/IgM rapid diagnostic test using an anti-dengue EDI monoclonal antibody and dengue virus antigen
Authors: Lee Jihoo , Kim Young-Eun , Kim Hak-Yong , Sinniah Mangalam , Chong Chom-Kyu , Song Hyun-Ok 
Published: December 2015

AbstractHigh levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI). The anti-dengue EDI mAb was employed as a capturer and EDII and EDIII, which are mainly involved in the induction of neutralizing antibodies in patients, were fully available to bind to anti-dengue IgM or IgG in patients. A one-way automatic blood separation device prevented reverse migration of plasma and maximize the capture of anti-dengue antibodies at the test lines. A clinical evaluation in the field proved that the novel RDT (sensitivities of 96.5% and 96.7% for anti-dengue IgM and IgG) is more effective in detecting anti-dengue antibodies than two major commercial tests (sensitivities of 54.8% and 82% for SD BIOLINE; 50.4% and 75.3% for PanBio). The innovative format of RDT can be applied to other infectious viral diseases.
Dipole-allowed direct band gap silicon superlattices
Authors: Oh Young Jun , Lee In-Ho , Kim Sunghyun , Lee Jooyoung , Chang Kee Joo 
Published: December 2015

AbstractSilicon is the most popular material used in electronic devices. However, its poor optical properties owing to its indirect band gap nature limit its usage in optoelectronic devices. Here we present the discovery of super-stable pure-silicon superlattice structures that can serve as promising materials for solar cell applications and can lead to the realization of pure Si-based optoelectronic devices. The structures are almost identical to that of bulk Si except that defective layers are intercalated in the diamond lattice. The superlattices exhibit dipole-allowed direct band gaps as well as indirect band gaps, providing ideal conditions for the investigation of a direct-to-indirect band gap transition. The fact that almost all structural portions of the superlattices originate from bulk Si warrants their stability and good lattice matching with bulk Si. Through first-principles molecular dynamics simulations, we confirmed their thermal stability and propose a possible method to synthesize the defective layer through wafer bonding.
One node driving synchronisation
Authors: Wang Chengwei , Grebogi Celso , Baptista Murilo S. 
Published: December 2015

AbstractAbrupt changes of behaviour in complex networks can be triggered by a single node. This work describes the dynamical fundamentals of how the behaviour of one node affects the whole network formed by coupled phase-oscillators with heterogeneous coupling strengths. The synchronisation of phase-oscillators is independent of the distribution of the natural frequencies, weakly depends on the network size, but highly depends on only one key oscillator whose ratio between its natural frequency in a rotating frame and its coupling strength is maximum. This result is based on a novel method to calculate the critical coupling strength with which the phase-oscillators emerge into frequency synchronisation. In addition, we put forward an analytical method to approximately calculate the phase-angles for the synchronous oscillators.
miR-140-5p regulates adipocyte differentiation by targeting transforming growth factor-β signaling
Authors: Zhang Xin , Chang Ailing , Li Yongmei , Gao Yifei , Wang Haixiao , Ma Zhongshu , Li Xiaoxia , Wang Baoli 
Published: December 2015

AbstractRecent emerging studies of miRNAs in adipocyte commitment provide new insights to understand the molecular basis of adipogenesis. The current study indicated that miR-140-5p was altered in primary cultured marrow stromal cells and established progenitor lines after adipogenic and/or osteogenic treatment. miR-140-5p was increased in adipose tissue in db/db obese mice vs. lean mice. Supplementing miR-140-5p activity induced stromal cell ST2 and preadipocyte 3T3-L1 to differentiate into mature adipocytes. Conversely, inhibition of the endogenous miR-140-5p repressed ST2 and 3T3-L1 to fully differentiate. By contrast, knockdown of the endogenous miR-140-5p enhanced osteoblast differentiation. Transforming growth factor-β receptor I (Tgfbr1) was shown to be a direct target of miR-140-5p. Supplementing miR-140-5p in ST2 reduced the level of TGFBR1 protein, while suppression of endogenous miR-140-5p increased TGFBR1. Overexpression of Tgfbr1 inhibited, whereas knockdown of Tgfbr1 promoted adipogenic differentiation of ST2 cells. Further investigation of mechanisms that control miR-140-5p expression revealed that C/EBPα induced transcriptional activity of the miR-140-5p promoter. Removal of the putative response element of C/EBP from the promoter abolished the enhancement of the promoter activity by C/EBPα, suggesting that C/EBPα transcriptionally controls miR-140-5p expression. Taken together, our study provides evidences that miR-140-5p regulates adipocyte differentiation through a C/EBP/miR-140-5p/TGFBR1 regulatory feedback loop.