bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light Sheet Theta Microscopy for High-resolution Quantitative Imaging of Large Biological Systems
Authors: Migliori Bianca , Datta Malika S. , Dupre Christophe , Apak Mehmet C. , Asano Shoh , Gao Ruixuan , Boyden Edward S. , Hermanson Ola , Yuste Rafael , Tomer Raju 
Published: March 2017

AbstractAdvances in tissue clearing and molecular labelling methods are enabling unprecedented optical access to large intact biological systems. These advances fuel the need for high-speed microscopy approaches to image large samples quantitatively and at high resolution. While Light Sheet Microscopy (LSM), with its high planar imaging speed and low photo-bleaching, can be effective, scaling up to larger imaging volumes has been hindered by the use of orthogonal light-sheet illumination. To address this fundamental limitation, we have developed Light Sheet Theta Microscopy (LSTM), which uniformly illuminates samples from same side as the detection objective, thereby eliminating limits on lateral dimensions without sacrificing imaging resolution, depth and speed. We present detailed characterization of LSTM, and show that this approach achieves rapid high-resolution imaging of large intact samples with superior uniform high-resolution than LSM. LSTM is a significant step in high-resolution quantitative mapping of structure and function of large intact biological systems.
Protein identification with a nanopore and a binary alphabet
Authors: Sampath G. 
Published: March 2017

AbstractProtein sequences are recoded with a binary alphabet obtained by dividing the 20 amino acids into two subsets based on volume. A protein is identified from subsequences by database search. Computations on theHelicobacter pyloriproteome show that over 93% of binary subsequences of length 20 are correct at a confidence level exceeding 90%. Over 98% of the proteins can be identified, most have multiple identifiers so the false detection rate is low. Binary sequences of unbroken protein molecules can be obtained with a nanopore from current blockade levels proportional to residue volume; only two levels, rather than 20, need be measured to determine a residue’s subset. This procedure can be translated into practice with a sub-nanopore that can measure residue volumes with ~0.07 nm3resolution as shown in a recent publication. The high detector bandwidth required by the high speed of a translocating molecule can be reduced more than tenfold with an averaging technique, the resulting decrease in the identification rate is only 10%. Averaging also mitigates the homopolymer problem due to identical successive blockade levels. The proposed method is a proteolysis-free single-molecule method that can identify arbitrary proteins in a proteome rather than specific ones. This approach to protein identification also works if residue mass is used instead of mass; again over 98% of the proteins are identified by binary subsequences of length 20. The possibility of using this in mass spectrometry studies of proteins, in particular those with post-translational modifications, is under investigation.
Cytosolic ribosomes on the surface of mitochondria
Authors: Gold Vicki A. M. , Chroscicki Piotr , Bragoszewski Piotr , Chacinska Agnieszka 
Published: March 2017

AbstractBy electron cryo-tomography and subtomogram averaging, translation-arrested ribosomes were used to depict the clustered organisation of the TOM complex on the surface of mitochondria, corroborating earlier reports of localized translation. Ribosomes were shown to interact specifically with the TOM complex and nascent chain binding was crucial for ribosome recruitment and stabilization. Ribosomes were bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport, and the importance of spatial organization for efficient mitochondrial protein import.
The cross-frequency mediation mechanism of intracortical information transactions
Authors: Pascual-Marqui RD , Faber P , Ikeda S , Ishii R , Kinoshita T , Kitaura Y , Kochi K , Milz P , Nishida K , Yoshimura M 
Published: March 2017

1. AbstractIn a seminal paper by von Stein and Sarnthein (2000), it was hypothesized that “bottom-up” information processing of “content” elicits local, high frequency (beta-gamma) oscillations, whereas “top-down” processing is “contextual”, characterized by large scale integration spanning distant cortical regions, and implemented by slower frequency (theta-alpha) oscillations. This corresponds to a mechanism of cortical information transactions, wheresynchronization of beta-gamma oscillations between distant cortical regions ismediatedby widespread theta-alpha oscillations. It is the aim of this paper to express this hypothesis quantitatively, in terms of a model that will allow testing this type of information transaction mechanism. The basic methodology used here corresponds to statistical mediation analysis, originally developed by (Baron and Kenny 1986). We generalize the classical mediator model to the case of multivariate complex-valued data, consisting of the discrete Fourier transform coefficients of signals of electric neuronal activity, at different frequencies, and at different cortical locations. The “mediation effect” is quantified here in a novel way, as the product of “dual frequency RV-coupling coefficients”, that were introduced in (Pascual-Marqui et al 2016,http://arxiv.org/abs/1603.05343). Relevant statistical procedures are presented for testing the cross-frequency mediation mechanism in general, and in particular for testing the von Stein & Sarnthein hypothesis.
The readability of scientific texts is decreasing over time
Authors: Plavén-Sigray Pontus , Matheson Granville James , Schiffler Björn Christian , Thompson William Hedley 
Published: March 2017

ABSTRACTClarity and accuracy of reporting are fundamental to the scientific process. The understandability of written language can be estimated using readability formulae. Here, in a corpus consisting of 707 452 scientific abstracts published between 1881 and 2015 from 122 influential biomedical journals, we show that the readability of science is steadily decreasing. Further, we demonstrate that this trend is indicative of a growing usage of general scientific jargon. These results are concerning for scientists and for the wider public, as they impact both the reproducibility and accessibility of research findings.
Marine fish intestine responds to ocean acidification producing more carbonate aggregates
Authors: Gregório Sílvia F. , Ruiz-Jarabo Ignacio , Carvalho Edison M , Fuentes Juan 
Published: March 2017

ABSTRACTMarine fish contribute to the carbon cycle by producing mineralized intestinal aggregates generated as by-products of their osmoregulation. Here we aimed at characterizing the control of intestinal aggregate production in the gilthead sea bream in response to near future increases of environmental CO2. Our results demonstrate that hypercapnia (800 and 1200 μatm CO2) elicits higher intestine epithelial HCO3−secretion and the subsequent parallel increase of intestinal aggregate production when compared to present values (400 μatm CO2). Intestinal gene expression analysis revealed the up-regulation of crucial transport mechanisms involved not only in the intestinal secretion cascade (Slc4a4, Slc26a3 and Slc26a6) of sea bream, but also in other mechanisms involved in intestinal ion uptake linked to water absorption such asNKCC2and theAquaporin 1b.These results highlight the important role of fish in the marine carbon cycle, and their potential growing impact of intestinal biomineralization processes in the scenario of ocean acidification.
Inflammatory Breast Cancer: a model for investigating cluster-based dissemination
Authors: Jolly Mohit Kumar , Boareto Marcelo , Debeb Bisrat G , Aceto Nicola , Farach-Carson Mary C , Woodward Wendy A , Levine Herbert 
Published: March 2017

AbstractMetastases claim more than 90% of cancer-related patient deaths and are usually seeded by a subset of circulating tumor cells (CTCs) shed off from the primary tumor. In circulation, CTCs are found both as single cells and as clusters of cells. The clusters of CTCs, although many fewer in number, possess much higher metastatic potential as compared to that of individual CTCs. In this review, we highlight recent insights into molecular mechanisms that can enable the formation of these clusters - (a) hybrid epithelial/mesenchymal (E/M) phenotype of cells that couples their ability to migrate and adhere, and (b) intercellular communication that can spatially coordinate the cluster formation and provide survival signals to cancer cells. Building upon these molecular mechanisms, we also offer a possible mechanistic understanding of why clusters are endowed with a higher metastatic potential. Finally, we discuss the highly aggressive Inflammatory Breast Cancer (IBC) as an example of a carcinoma that can metastasize via clusters and corroborates the proposed molecular mechanisms.
Evaluating the adaptive reuse potential of buildings in conservation areas
Authors: Hong Yan , Chen Fei 
Published: March 2017

Purpose
Adaptive reuse of building assets is an important approach to sustainability. Adapting a building for new uses often involves complex factors in the decision-making process, particularly in conservation areas. The paper aims to show an evaluation process of the adaptive reuse potential of historic buildings that are subject to change in the Grand Canal area, a world heritage site in Hangzhou, China.

Design/methodology/approach
For this purpose, a model has been established with aggregated views of professionals on the degree to which a variety of factors affect the buildings’ potential for adaptive reuse. The model intends to help prioritise some of the buildings in the area for adaptive reuse, which is important for effective allocation of public resources. Interviews with professionals, analytic hierarchy process and the Delphi method have been used to establish the evaluation model. It is then applied to the Grand Canal area to generate indices for buildings’ adaptive reuse potential and the ranking of priority. The indices are generated through public scoring of historic buildings against the variables and calculated through the model.

Findings
The paper concludes that the evaluation process is an effective way to engage the public in the decision-making process and to balance conflict interests of various stakeholders in the management of historic building assets in conservation areas.

Originality/value
The research has proposed an evaluation model to help set priority of buildings subject to adaptive reuse and to help distribute public fund effectively. It facilitates wide public engagement in the decision-making progress of adaptive reuse of historic buildings.
Investigating training through the lens of dramatic possibilities
Authors: Shree Sonal 
Published: March 2017

Purpose
The purpose of this paper is to probe aid readers’ understanding of the areas in which drama-based trainings are being used and how drama as a tool acts as a means to achieve desired learning and behavioral changes in organizations.

Design/methodology/approach
The paper uses literature review to analyze the practice of drama-based training.

Findings
Drama-based training is one of the most effective tools to engage participants. It elicits the desired response in them when they ruminate over familiar circumstances or behaviors being depicted and leads to instant recall and associations that acts as a propeller to imbibing new learning. Coming up with an alternative response or behavior as a result of experiencing events through drama could help them learn or manage the situation through behavioral transformation.

Research limitations/implications
Being conceptual in nature, this model must be tested empirically by relevant stakeholders in the area of learning and development to add further weightage to literature.

Practical implications
By detailing drama-based training used in varied areas of specialization and how its usage has tremendous potential to facilitate bringing about desired behavioral changes, the paper demonstrates the importance of creating a lasting impact through this method of training that will specially be relevant to HRD managers.

Originality/value
Multidisciplinary areas in which drama- or theatre-based trainings are being used have been studied through literature review and a conceptual model of training, abbreviated as DRAMA for easy recall, has been proposed with inclusion of salient features that make drama-based interventions for training so engaging and effective for disseminating learning. This model also finds some connection with the Kolb’s experimental learning theory.
“The great divide”: skill gap between the employer’s expectations and skills possessed by employees
Authors: Malik Geetika , Venkatraman A. 
Published: March 2017

Purpose
The purpose of this paper is to emphasise on demand supply skill gap of human resources in India which would help generate reasons for the same and the necessary steps to be taken to improve the situation and bridge the skill gap helping maximum number of the students becoming employable. It will put an impact on various stakeholders about the steps to be taken in bridging the gap in India.

Design/methodology/approach
A review of available literature was done in order to understand the reasons behind the increasing skill gap despite a vibrant and abundance of young population.

Findings
The findings of the paper implicitly point out that a vast skill gap is prevailing in the Indian labour market and there is a dearth of human intellectual capital in India. Also it emphasises on the role of various stakeholders in bridging the skill gap by investing in workforce of today for a better tomorrow.

Originality/value
Based on literature review, the paper makes specific recommendations to various stakeholders (industry, academia, and government) in bridging the skill gap and would serve as a roadmap for companies guiding them to follow certain strategies to improve skills of future workers by investing in them and growing their own talent pool; and is an attempt to highlight the fact that India’s jobless growth is not going to reap the demographic dividend and certainly the increasing skill gap needs to be bridged ASAP.
Cost, revenue and profit efficiency analysis of Indian scheduled commercial banks
Authors: Mahendru Megha , Bhatia Aparna 
Published: March 2017

Purpose
This paper aims to analyze the cost, revenue and profit efficiency performance of Indian scheduled commercial banks. The study also determines differences if any related to efficiency among banks on the basis of ownership pattern.

Design/methodology/approach
Cost, revenue and profit efficiency of banks is calculated by using the non-parametric approach, namely, data envelopment analysis. Further, the differences in the efficiency scores are examined by applying analysis of variance.

Findings
Indian scheduled commercial banks have not been able to maintain their input-output synchronization in terms of cost, revenue and profits in the year 2012-2013. Foreign sector banks have higher cost and profit efficiency as compared to their counterparts in private and public sector, whereas public sector banks are found to have been more revenue efficient.

Originality/value
With specific reference to India, less empirical work has been carried out with respect to cost, revenue and profit efficiency. None of the studies have evaluated the sector-wise performance of banks in terms of all three efficiency parameters.
Impact of worksite cafeteria interventions on fruit and vegetable consumption in adults
Authors: Hendren Sarah , Logomarsino John 
Published: March 2017

PurposeIncreasing obesity rates and health care costs have prompted worksites to investigate interventions to improve employee health. The purpose of this paper is to determine the effect of worksite cafeteria interventions on fruit and vegetable (F/V) consumption.Design/methodology/approachThis review was guided by the preferred reporting items for systematic reviews and meta-analyses method. After a thorough literature search and screening process, 18 studies were included in the review. Data were extracted, and a risk of bias assessment was created for the primary studies. An un-weighted average was used to determine the overall ranking for each study.FindingsThere appears to be a moderately strong association toward a positive impact of cafeteria interventions to increase F/V consumption. Of the 18 studies in the review, 13 reported a statistically significant increase, one reported a significant decrease, three reported mixed results, and one did not assess a change in consumption.Research limitations/implicationsMost of the data were self-reported and is subject to error. Furthermore, the heterogeneity of study design, method, and outcome measures among the studies warrants additional research with consistent methodology.Practical implicationsA positive impact on F/V consumption may be realized by the following techniques: price-point subsidies, point-of-purchase materials, and menu modification.Originality/valueThis new information on increasing F/V consumption in workplace cafeterias may improve employee health and reduce the risk of chronic disease.
Tribology study of hydroxyapatite (HAp) scaffold blended single based binder via rheology and mechanical properties
Authors: Abu Bakar Mimi Azlina , Abd Aziz Siti Norazlini , Ismail Muhammad Hussain 
Published: March 2017

Purpose
This paper aims to investigate the vital characteristic of an innovative ceramic injection molding (CIM) process for orthopedic application with controlled porosity and improved tribological and mechanical properties which were affected by complex tribological interactions, whether lubricated like hip implants and other artificial prostheses. The main objective is to maximize the usage of palm stearin as a single based binder as the function of flow properties during injection molding process.

Design/methodology/approach
The binder used in this present study consists of 100 per cent palm stearin manufactured by Kempas Oil Sdn Bhd and supplied by Vistec Technology Sdn Bhd. The feedstock was prepared by using a Z-blade mixer (Thermo Haake Rheomix OS) and Brabender mixer model R2400. The feedstock prepared was injection molded using a manually operated vertical benchtop machine with an average pressure of about 5-7 bars. The firing step included the temporary holds at intermediate temperatures to burn out organic binders. At this stage, the green molded specimen was de-bound using a single-step wick-debinding method.

Findings
The maximum content of ceramic material is applied to investigate the efficiencies of net formulation that can be achieved by ceramic materials. The longer the viscosity will change with shear rate, the higher the value of n obtained instead. From the slope of the curves obtained in Figure 3, the value of n for the feedstock was determined to be less than 1, which indicates a pseudoplastic behavior and suitability for the molding process. Moreover, high shear sensitivity is important in producing complex and intrinsic specimens which are leading products in the CIM industry.

Originality/value
The feedstock containing HAp powder and palm stearin binder was successfully prepared at very low temperature of 70°C, which promoting a required pseudo-plastic behavior during rheological test. The single binder palm stearin should be optimized in other research works carried out, as palm stearin is most preferred compared to other polymeric materials that provided high energy consumption when subjected to the sintering process. Besides the binder is widely available in Malaysia, low cost and harmless effect during debinding process.
China’s foreign trade policy with OPEC member countries
Authors: Rasoulinezhad Ehsan 
Published: March 2017

PurposeThe purpose of this paper is to analyze specifications of the China’s foreign trade policy with Organization of the Petroleum Exporting Countries (OPEC) member countries.Design/methodology/approachThe paper conducts three panel data estimations (fixed effect [FE], random effect [RE] and fully modified ordinary least squares [FMOLS]) based on the gravity model approach for bilateral trade patterns in natural resource and non-natural resource commodities between China and 13 OPEC members over the period of 1998-2014.FindingsThe findings reveal that the gravity equation fits the data reasonably well. The existence of long-term relationships between the bilateral trade flows and the main components of gravity model – GDP, income (GDP per capita), the difference in income, exchange rate, the openness level, distance and WTO membership – through the FE, RE and the FMOLS approaches was confirmed. The estimation results show that the trade pattern between China and OPEC member countries relies on the Heckscher–Ohlin theory, thus being explained by difference in factor endowments such as energy resources and technology.Originality/valueTo the best of the authors’ knowledge, this is the first attempt to examine the China’s foreign trade policy with the OPEC member countries through a gravity trade approach.
Change your space, change your culture: exploring spatial change management strategies
Authors: Skogland Mari Anna Chatarina , Hansen Geir Karsten 
Published: March 2017

Purpose
The purpose of the paper is to explore the use of, and challenges associated with, spatial change management strategies. This is done through a discussion on how spatial environments may be utilised to effect organisational change. The intention is to provoke new thinking on physical change initiatives and to challenge the often highly deterministic view on the effects of contemporary workspace concepts.

Design/methodology/approach
The paper is structured as a case study-based literature review, drawing on literature from the fields of environmental psychology, organisational branding, corporate real estate and facility management, as well as organisational change management.

Findings
The study indicates that space management strategies may fail because of the lack of understanding of how organisational events and other contextually specific aspects correlate with the physical change initiative. Succeeding with the spatial strategy requires a strong focus on socio-material relationships and the employee meaning-making process during the spatial change process.

Originality/value
Contrary to the traditional and rational focus on functional space management strategies, the paper takes a socio-material approach suggesting that there is a need for more empirically based research into the employee meaning-making process and the role of human and organisational practices in the development of new workplace concepts. Focusing on how organisational members understand and “make use of” spatial environments may substantially improve organisations and building consultants’ abilities to strategically manage the physical change initiative and achieve the intended ends.