bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anaerobic Capacityestimated in A Single Supramaximal Test in Cycling: Validity and Reliability Analysis
Authors: Miyagi Willian Eiji , de Poli Rodrigo de Araujo Bonetti , Papoti Marcelo , Bertuzzi Romulo , Zagatto Alessandro Moura 
Published: February 2017

AbstractThe aim was to verify the validity (i.e., study A) and reliability (i.e., study B) of the alternative maximal accumulated oxygen deficit determined using onlya supramaximal effort (MAODALT)to estimate anaerobic capacity [i.e., estimated by the gold standard maximal accumulated oxygen deficit method (MAOD)] during cycling. In study A, the effects of supramaximal intensities on MAODALT and the comparison with the MAOD were investigated in fourteen active subjects (26 ± 6 years). In study B, the test-retest reliability was investigated, where fourteen male amateur cyclists (29 ± 5 years) performed the MAODALT twice at 115% of the intensity associated to maximal oxygen uptake ("Equation missing"). MAODALT determined at 130 and 150% of "Equation missing" was lower than MAOD (p ≤ 0.048), but no differences between MAODALT determined at 100, 105, 110, 115, 120 and 140% of "Equation missing" (3.58 ± 0.53L; 3.58 ± 0.59L; 3.53 ± 0.52L; 3.48 ± 0.72L; 3.52 ± 0.61L and 3.46 ± 0.69L, respectively) with MAOD (3.99 ± 0.64L). The MAODALT determined from the intensities between 110 and 120% of "Equation missing" presented the better agreement and concordance with MAOD. In the test-retest, the MAODALT was not different (p > 0.05), showed high reproducibility when expressed in absolute values (ICC = 0.96, p < 0.01), and a good level of agreement in the Bland-Altman plot analysis (mean differences ± CI95%:−0.16 ± 0.53L). Thus, the MAODALT seems to be valid and reliable to assess anaerobic capacity in cycling.
The N-terminal domain of Schmallenberg virus envelope protein Gc is highly immunogenic and can provide protection from infection
Authors: Wernike Kerstin , Aebischer Andrea , Roman-Sosa Gleyder , Beer Martin 
Published: February 2017

AbstractSchmallenberg virus (SBV) is transmitted by insect vectors, and therefore vaccination is one of the most important tools of disease control. In our study, novel subunit vaccines on the basis of an amino-terminal domain of SBV Gc of 234 amino acids (“Gc Amino”) first were tested and selected using a lethal small animal challenge model and then the best performing formulations also were tested in cattle. We could show that neither E. coli expressed nor the reduced form of “Gc Amino” protected from SBV infection. In contrast, both, immunization with “Gc Amino”-encoding DNA plasmids and “Gc-amino” expressed in a mammalian system, conferred protection in up to 66% of the animals. Interestingly, the best performance was achieved with a multivalent antigen containing the covalently linked Gc domains of both, SBV and the related Akabane virus. All vaccinated cattle and mice were fully protected against SBV challenge infection. Furthermore, in the absence of antibodies against the viral N-protein, differentiation between vaccinated and field-infected animals allows an SBV marker vaccination concept. Moreover, the presented vaccine design also could be tested for other members of the Simbu serogroup and might allow the inclusion of additional immunogenic domains.
Experimental observation of spatially resolved photo-luminescence intensity distribution in dual mode upconverting nanorod bundles
Authors: Kumar Pawan , Singh Satbir , Singh V. N. , Singh Nidhi , Gupta R. K. , Gupta Bipin Kumar 
Published: February 2017

AbstractA novel method for demonstration of photoluminescence intensity distribution in upconverting nanorod bundles using confocal microscopy is reported. Herein, a strategy for the synthesis of highly luminescent dual mode upconverting/downshift Y1.94O3:Ho3+0.02/Yb3+0.04 nanorod bundles by a facile hydrothermal route has been introduced. These luminescent nanorod bundles exhibit strong green emission at 549 nm upon excitations at 449 nm and 980 nm with quantum efficiencies of ~6.3% and ~1.1%, respectively. The TEM/HRTEM results confirm that these bundles are composed of several individual nanorods with diameter of ~100 nm and length in the range of 1–3 μm. Furthermore, two dimensional spatially resolved photoluminescence intensity distribution study has been carried out using confocal photoluminescence microscope throughout the nanorod bundles. This study provides a new direction for the potential use of such emerging dual mode nanorod bundles as photon sources for next generation flat panel optical display devices, bio-medical applications, luminescent security ink and enhanced energy harvesting in photovoltaic applications.
Design of a Porous Cathode for Ultrahigh Performance of a Li-ion Battery: An Overlooked Pore Distribution
Authors: Song Jihwan , Kim Junhyung , Kang Taewook , Kim Dongchoul 
Published: February 2017

AbstractTypical cathode materials of Li-ion battery suffer from a severe loss in specific capacity, and this problem is regarded as a major obstacle in the expansion of newer applications. To overcome this, porous cathodes are being extensively utilized. However, although it seems that the porosity in the cathode would be a panacea for high performance of LIBs, there is a blind point in the cathode consisting of porous structures, which makes the porous design to be a redundant. Here, we report the importance of designing the porosity of a cathode in obtaining ultrahigh performance with the porous design or a degraded performance even with increase of porosity. Numerical simulations show that the cathode with 40% porosity has 98% reduction in the loss of specific capacity when compared to the simple spherical cathode when the C-rate increases from 2.5 to 80 C. In addition, the loss over total cycles decreases from 30% to only about 1% for the cathode with 40% porosity under 40 C. Interestingly, however, the specific capacity could be decreased even with the increase in porosity unless the pores were evenly distributed in the cathode. The present analysis provides an important insight into the design of ultrahigh performance cathodes.
Electroacupuncture Attenuates CFA-induced Inflammatory Pain by suppressing Nav1.8 through S100B, TRPV1, Opioid, and Adenosine Pathways in Mice
Authors: Liao Hsien-Yin , Hsieh Ching-Liang , Huang Chun-Ping , Lin Yi-Wen 
Published: February 2017

AbstractPain is associated with several conditions, such as inflammation, that result from altered peripheral nerve properties. Electroacupuncture (EA) is a common Chinese clinical medical technology used for pain management. Using an inflammatory pain mouse model, we investigated the effects of EA on the regulation of neurons, microglia, and related molecules. Complete Freund’s adjuvant (CFA) injections produced a significant mechanical and thermal hyperalgesia that was reversed by EA or a transient receptor potential V1 (TRPV1) gene deletion. The expression of the astrocytic marker glial fibrillary acidic protein (GFAP), the microglial marker Iba-1, S100B, receptor for advanced glycation end-products (RAGE), TRPV1, and other related molecules was dramatically increased in the dorsal root ganglion (DRG) and spinal cord dorsal horn (SCDH) of CFA-treated mice. This effect was reversed by EA and TRPV1 gene deletion. In addition, endomorphin (EM) and N6-cyclopentyladenosine (CPA) administration reliably reduced mechanical and thermal hyperalgesia, thereby suggesting the involvement of opioid and adenosine receptors. Furthermore, blocking of opioid and adenosine A1 receptors reversed the analgesic effects of EA. Our study illustrates the substantial therapeutic effects of EA against inflammatory pain and provides a novel and detailed mechanism underlying EA-mediated analgesia via neuronal and non-neuronal pathways.
Graphene Oxide Demonstrates Experimental Confirmation of Abraham Pressure on Solid Surface
Authors: Kundu Anirban , Rani Renu , Hazra Kiran S. 
Published: February 2017

AbstractThe century-old controversy over two contradicting theories on radiation pressure of light proposed by Abraham and Minkowski can come to an end if there is a direct method to measure the surface deformation of the target material due to momentum transfer of photons. Here we have investigated the effect of radiation pressure on the surface morphology of Graphene Oxide (GO) film, experienced due to low power focused laser irradiation. In-depth investigation has been carried out to probe the bending of the GO surface due to radiation pressure by Atomic Force Microscopy (AFM) and subsequently the uniaxial strain induced on the GO film has been probed by Raman Spectroscopy. Our results show GO film experience an inward pressure due to laser radiation resulting in inward bending of the surface, which is consistent with the Abraham theory. The bending diameter and depth of the irradiated spot show linear dependence with the laser power while an abrupt change in depth and diameter of the irradiated spot is observed at the breaking point. Such abrupt change in depth is attributed to the thinning of the GO film by laser irradiation.
Simultaneous observation of particle and wave behaviors of entangled photons
Authors: Man Zhong-Xiao , Xia Yun-Jie , An Nguyen Ba 
Published: February 2017

AbstractWe theoretically study wave-particle duality of two entangled photons in the spirit of quantum version of delayed-choice experiments using Hadamard gate controlled by the quantum state of an ancilla and show that the two photons may globally exhibit particle-like, wave-like or simultaneously both particle-like and wave-like behavior. We prove that the obtained results cannot be satisfactorily explained by any hidden-variable theory. We also propose an efficient and experimentally feasible scheme without using any ancilla and controlled-gates to directly (i.e., without postselection) observe the two-photon wave-particle superposed state as well as the continuous transition of their behavior between wave-like one and particle-like one.
Role of endothelial nitric oxide synthase and endothelin-1 polymorphism genes with the pathogenesis of intraventricular hemorrhage in preterm infants
Authors: Szpecht Dawid , Gadzinowski Janusz , Seremak-Mrozikiewicz Agnieszka , Kurzawińska Grażyna , Szymankiewicz Marta 
Published: February 2017

AbstractIn the pathogenesis of neonatal intraventricular hemorrhage (IVH) in preterm infants, an important role is played by changes in venous and arterial cerebral flows. It has been shown that the ability of autoregulation of cerebral flows in response to variations in arterial blood pressure in preterm infants is impaired. This impaired autoregulation causes an increased risk of germinal matrix rupture and IVH occurrence. We examined three polymorphisms of genes, related to regulation of blood flow, for an association with IVH in 100 preterm infants born from singleton pregnancy, before 32 + 0 weeks of gestation, exposed to antenatal steroids therapy, and without congenital abnormalities. These polymorphisms include: eNOS (894G > T and −786T > C) and EDN1 (5665G > T ) gene. We found that infants with genotype GT eNOS 894G > T have 3.4-fold higher risk developing of IVH born before 28 + 6 weeks of gestation. Our investigation did not confirm any significant prevalence for IVH development according to eNOS −786T > C genes polymorphism. Our novel investigations in EDN1 5665G > T polymorphism did not show any link between alleles or genotypes and IVH. Future investigations of polymorphisms in blood-flow associated genes may provide valuable insight into the pathogenetic mechanisms underlying the development of IVH.
Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease
Authors: Zhang Likui , Xu Dandan , Huang Yanchao , Zhu Xinyuan , Rui Mianwen , Wan Ting , Zheng Xin , Shen Yulong , Chen Xiangdong , Ma Kesen , Gong Yong 
Published: February 2017

AbstractHNH endonucleases in bacteriophages play a variety of roles in the phage lifecycle as key components of phage DNA packaging machines. The deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2) encodes an HNH endonuclease (GVE2 HNHE). Here, the crystal structure of GVE2 HNHE is reported. This is the first structural study of a thermostable HNH endonuclease from a thermophilic bacteriophage. Structural comparison reveals that GVE2 HNHE possesses a typical ββα-metal fold and Zn-finger motif similar to those of HNH endonucleases from other bacteriophages, apart from containing an extra α-helix, suggesting conservation of these enzymes among bacteriophages. Biochemical analysis suggests that the alanine substitutions of the conserved residues (H93, N109 and H118) in the HNH motif of GVE2 HNHE abolished 94%, 60% and 83% of nicking activity, respectively. Compared to the wild type enzyme, the H93A mutant displayed almost the same conformation while the N108A and H118A mutants had different conformations. In addition, the wild type enzyme was more thermostable than the mutants. In the presence of Mn2+ or Zn2+, the wild type enzyme displayed distinct DNA nicking patterns. However, high Mn2+ concentrations were needed for the N109A and H118A mutants to nick DNA while Zn2+ inactivated their nicking activity.
Amyloid-β Oligomers Interact with Neurexin and Diminish Neurexin-mediated Excitatory Presynaptic Organization
Authors: Naito Yusuke , Tanabe Yuko , Lee Alfred Kihoon , Hamel Edith , Takahashi Hideto 
Published: February 2017

AbstractAlzheimer’s disease (AD) is characterized by excessive production and deposition of amyloid-beta (Aβ) proteins as well as synapse dysfunction and loss. While soluble Aβ oligomers (AβOs) have deleterious effects on synapse function and reduce synapse number, the underlying molecular mechanisms are not well understood. Here we screened synaptic organizer proteins for cell-surface interaction with AβOs and identified a novel interaction between neurexins (NRXs) and AβOs. AβOs bind to NRXs via the N-terminal histidine-rich domain (HRD) of β-NRX1/2/3 and alternatively-spliced inserts at splicing site 4 of NRX1/2. In artificial synapse-formation assays, AβOs diminish excitatory presynaptic differentiation induced by NRX-interacting proteins including neuroligin1/2 (NLG1/2) and the leucine-rich repeat transmembrane protein LRRTM2. Although AβOs do not interfere with the binding of NRX1β to NLG1 or LRRTM2, time-lapse imaging revealed that AβO treatment reduces surface expression of NRX1β on axons and that this reduction depends on the NRX1β HRD. In transgenic mice expressing mutated human amyloid precursor protein, synaptic expression of β-NRXs, but not α-NRXs, decreases. Thus our data indicate that AβOs interact with NRXs and that this interaction inhibits NRX-mediated presynaptic differentiation by reducing surface expression of axonal β-NRXs, providing molecular and mechanistic insights into how AβOs lead to synaptic pathology in AD.
High-Performance Regular Perovskite Solar Cells Employing Low-Cost Poly(ethylenedioxythiophene) as a Hole-Transporting Material
Authors: Jiang Xiaoqing , Yu Ze , Zhang Yuchen , Lai Jianbo , Li Jiajia , Gurzadyan Gagik G. , Yang Xichuan , Sun Licheng 
Published: February 2017

AbstractHerein, we successfully applied a facile in-situ solid-state synthesis of conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) as a HTM, directly on top of the perovskite layer, in conventional mesoscopic perovskite solar cells (PSCs) (n-i-p structure). The fabrication of the PEDOT film only involved a very simple in-situ solid-state polymerisation step from a monomer 2,5-dibromo-3,4-ethylenedioxythiophene (DBEDOT) made from a commercially available and cheap starting material. The ultraviolet photoelectron spectroscopy (UPS) demonstrated that the as-prepared PEDOT film possesses the highest occupied molecular orbital (HOMO) energy level of −5.5 eV, which facilitates an effective hole extraction from the perovskite absorber as confirmed by the photoluminescence measurements. Optimised PSC devices employing this polymeric HTM in combination with a low-cost vacuum-free carbon cathode (replacing the gold), show an excellent power conversion efficiency (PCE) of 17.0% measured at 100 mW cm−2 illumination (AM 1.5G), with an open-circuit voltage (Voc) of 1.05 V, a short-circuit current density (Jsc) of 23.5 mA/cm2 and a fill factor (FF) of 0.69, respectively. The present finding highlights the potential application of PEDOT made from solid-state polymerisation as a HTM for cost-effective and highly efficient PSCs.
Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models
Authors: Lakner Pirmin H. , Monaghan Michael G. , Möller Yvonne , Olayioye Monilola A. , Schenke-Layland Katja 
Published: February 2017

AbstractFluorescence lifetime imaging microscopy (FLIM) can measure and discriminate endogenous fluorophores present in biological samples. This study seeks to identify FLIM as a suitable method to non-invasively detect a shift in cellular metabolic activity towards glycolysis or oxidative phosphorylation in 3D Caco-2 models of colorectal carcinoma. These models were treated with potassium cyanide or hydrogen peroxide as controls, and epidermal growth factor (EGF) as a physiologically-relevant influencer of cell metabolic behaviour. Autofluorescence, attributed to nicotinamide adenine dinucleotide (NADH), was induced by two-photon laser excitation and its lifetime decay was analysed using a standard multi-exponential decay approach and also a novel custom-written code for phasor-based analysis. While both methods enabled detection of a statistically significant shift of metabolic activity towards glycolysis using potassium cyanide, and oxidative phosphorylation using hydrogen peroxide, employing the phasor approach required fewer initial assumptions to quantify the lifetimes of contributing fluorophores. 3D Caco-2 models treated with EGF had increased glucose consumption, production of lactate, and presence of ATP. FLIM analyses of these cultures revealed a significant shift in the contribution of protein-bound NADH towards free NADH, indicating increased glycolysis-mediated metabolic activity. This data demonstrate that FLIM is suitable to interpret metabolic changes in 3D in vitro models.
Tobacco Influence on Taste and Smell: Systematic Review of the Literature
Authors: Da Ré Allessandra , Gurgel Léia , Buffon Gabriela , Moura Weluma , Marques Vidor Deisi , Maahs Márcia 
Published: February 2017

Introduction In Brazil, estimates show that 14.7% of the adult population smokes, and changes in smell and taste arising from tobacco consumption are largely present in this population, which is an aggravating factor to these dysfunctions.
Objectives The objective of this study is to systematically review the findings in the literature about the influence of smoking on smell and taste.
Data Synthesis Our research covered articles published from January 1980 to August 2014 in the following databases: MEDLINE (accessed through PubMed), LILACS, Cochrane Library, and SciELO. We conducted separate lines of research: one concerning smell and the other, taste. We analyzed all the articles that presented randomized controlled studies involving the relation between smoking and smell and taste. Articles that presented unclear methodologies and those whose main results did not target the smell or taste of the subjects were excluded. Titles and abstracts of the articles identified by the research strategy were evaluated by researchers. We included four studies, two of which were exclusively about smell: the first noted the relation between the perception of puff strength and nicotine content; the second did not find any differences in the thresholds and discriminative capacity between smokers and nonsmokers. One article considered only taste and supports the relation between smoking and flavor, another considered both sensory modalities and observes positive results toward the relation immediately after smoking cessation.
Conclusion Three of the four studies presented positive results for the researched variables.
Accuracy and Learning Curve of Femoral Tunnel Placement in Medial Patellofemoral Ligament Reconstruction
Authors: Hiemstra Laurie , O'Brien Catherine , Lafave Mark , Kerslake Sarah 
Published: February 2017

AbstractThe purpose of this study was to assess the accuracy of femoral tunnel placement in a medial patellofemoral ligament reconstruction (MPFL-R) cohort. The secondary purpose was to establish the evidence of a learning curve to achieve acceptable femoral tunnel placement during MPFL-R. Two surgeons, using lateral radiographs, assessed 73 subjects post–MPFL-R. Femoral tunnel accuracy and direction of tunnel error were measured in relation to Schöttle's point (A–T distance). Interrater reliability (intraclass correlation coefficient 2,k) of these measures was calculated. Learning curve of accurate femoral tunnel placement was examined by dividing the patient cohort into quartiles. A one-way analysis of variance was used to assess the quartiles for accuracy of femoral tunnel position and surgical time. In relation to Schöttle's point, 66/73 (90.4%) femoral tunnels were categorized as being in a “good” or “excellent” position and 7/73 (9.6%) were categorized as being in a “poor” position. Evidence of an MPFL-R learning curve was established via a statistically significant difference in the mean A to T distance for the four quartiles (F [3, 69] = 6.41, p = 0.001). There was also a statistically significant difference in the surgical time for the four quartiles (F [3, 69] = 8.71, p = 0.001). In this series, accurate femoral tunnels were placed more than 90% of the time during MPFL-R. A clear learning curve for accurate femoral tunnel placement was demonstrated both with respect to distance of the tunnel from Schöttle point and with regard to surgical time. Level of evidence was IV.
Lenticulostriate Vasculopathy and Neurodevelopmental Outcomes in Preterm Infants: A Systematic Review
Authors: Robinson Abby , Flibotte John , Kaplan Summer , DeMauro Sara 
Published: February 2017

Objective The objective of this study was to perform a systematic review of all studies that report neurodevelopmental outcomes at 12 months' corrected age or later for preterm infants (< 32 weeks) who are diagnosed with lenticulostriate vasculopathy (LSV) on cranial ultrasound.
Study Design This is a systematic review.
Results We identified 501 unique publications of which 3 met all prespecified eligibility criteria. Neurodevelopmental outcome data were available for 44 preterm infants with LSV. There were mixed results from the studies: the first reported impairments of mental development; the second reported impairments of motor development only; and finally, the third reported that three of three preterm infants with LSV had mild or moderate developmental delay.
Conclusion The available results raise some concern for future impairments in preterm infants with LSV, but they are conflicting and inconclusive. There is insufficient evidence about the neurodevelopmental implications of LSV in preterm infants to inform counseling of parents.