bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depression with inflammation: longitudinal analysis of a proposed depressive subtype in community dwelling older adults
Authors: Gallagher Damien , Kiss Alex , Lanctot Krista , Herrmann Nathan 
Published: December 2016

ObjectiveIt has been proposed that inflammation may be causally related to depression. If this is the case, it may be possible to distinguish an inflammatory depressive subtype according to illness course, pattern of co‐morbidity and symptom profile.MethodsEight hundred and eleven community dwelling older adults with depression (8 item Center for Epidemiologic Studies scale ≥ 4) from the English Longitudinal study of Ageing (ELSA) were followed for a median of 47 months. Participants with depression and inflammation (C Reactive Protein > 3 mg/l) were compared to those with depression alone.ResultsIn a longitudinal analysis, depression with associated inflammation was more likely to persist over time. This association was independent of baseline depression severity and medical co‐morbidity (OR 1.47 95% CI 1.03 – 2.10, p = 0.034) but was no longer significant following further adjustment for body mass index (OR 1.37 95% CI 0.94 – 2.01, p = 0.106). Inflammation either partially or completely mediated the association between medical co‐morbidity, body mass index and depression at follow‐up. Depression with inflammation was associated with more amotivation, less sadness, greater medical co‐morbidity and higher body mass index.ConclusionsOur findings provide some support for an inflammatory contribution to depression. This subgroup has a worse prognosis and may benefit from interventions targeting co‐morbidity, body mass index and associated inflammation. Copyright © 2016 John Wiley & Sons, Ltd.
Degradation and biocompatibility of photoembossed PLGA–acrylate blend for improved cell adhesion
Authors: Hughes‐Brittain Nanayaa F. , Qiu Lin , Wang Wen , Peijs Ton , Bastiaansen Cees W. M. 
Published: December 2016

AbstractWe have shown previously that PMMA–acrylate photopolymers are biocomopatible and can exhibit improved cell adhesion compared to PMMA, due to an increase in negative surface charge caused by UV radiation PLGA has been used widely in soft tissue regeneration due to its high biocompatibility and cell adhesion. This polymer is also biodegradable and can be utilised in the field of vascular regeneration. In this study, PLGA is blended with a triacrylate monomer (TPETA) to create a degradable photopolymer blend. Surface relief structures are formed on this PLGA–TPETA by photoembossing. An optimum height of 950 nm was achieved for a 10 µm pitch with the height of these relief structures being controlled by changing UV intensity, processing temperature and time. Degradation studies of this blend revealed a bulk degradation mechanism with PLGA–TPETA degrading slower compared to pure PLGA. We also evaluated the adhesion of human umbilical vein endothelial cells (HUVECs) on both smooth and textured PLGA–TPETA films. Embossed PLGA–TPETA films showed improved cell adhesion compared to smooth substrates. Furthermore, HUVECs proliferated faster on the embossed surface compared to their smooth counterparts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 163–171, 2018.
Congenital glaucoma detected by unilateral megalophthalmos on prenatal sonography
Authors: Gennette Sarah , Carlan Steve J. , Busowski John , Madruga Mario , McWhorter Jeannie 
Published: December 2016

ABSTRACTA 36‐week male fetus was noted to have isolated right megalophthalmos on prenatal ultrasound and was found to have buphthalmos and congenital glaucoma at birth. Detection of congenital glaucoma at birth may be occasionally possible if abnormal orbit dimensions are noted on late prenatal sonographic examination. Early neonatal intervention may improve the chances to retain vision. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 45:499–501, 2017
The Mediating Roles of the Overall Perceived Value of the Ecotourism Site and Attitudes Towards Ecotourism in Sustainability Through the Key Relationship Ecotourism Knowledge‐Ecotourist Satisfaction
Authors: Oviedo‐García M. Ángeles , Castellanos‐Verdugo Mario , Vega‐Vázquez Manuela , Orgaz‐Agüera Francisco 
Published: December 2016

AbstractTourist satisfaction is a key factor in tourism sustainability. A multiple mediation model is proposed to analyze the relationship between ecotourism knowledge and ecotourist satisfaction, assisting our understanding of the generative mechanism by which ecotourism knowledge influences ecotourist satisfaction. Ecotourists at the Natural Park Saltos de la Damajagua (Dominican Republic) were administered a questionnaire. The results (PLS‐SEM) show that ecotourism site perceived value and attitudes towards ecotourism fully mediate the influence of ecotourism knowledge on ecotourist satisfaction. Ecotourism knowledge will therefore only have positive effects on ecotourist satisfaction when the ecotourism site is of high perceived value and there are positive attitudes towards ecotourism. Copyright © 2016 John Wiley & Sons, Ltd.
Visible Laser‐Induced In Situ Cell Detachment from Gold Nanoparticle‐Embedded Collagen Gel
Authors: Kojima Chie , Nakajima Yusuke , Oeda Naoya , Kawano Takeshi , Taki Yusuke 
Published: December 2016

Cell sorting is important for cell biology and regenerative medicine. A visible light‐responsive cell scaffold is produced using gold nanoparticles and collagen gel. Various kinds of cells are cultured on the visible light‐responsive cell scaffold, and the target cells are selectively detached by photoirradiation without any cytotoxicity. This is a new image‐guided cell sorting system.
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Membrane defects and genetic redundancy: Are we at a turning point for DYT1 dystonia?
Authors: Cascalho Ana , Jacquemyn Julie , Goodchild Rose E. 
Published: December 2016

ABSTRACTHeterozygosity for a 3‐base pair deletion (ΔGAG) in TOR1A/torsinA is one of the most common causes of hereditary dystonia. In this review, we highlight current understanding of how this mutation causes disease from research spanning structural biochemistry, cell science, neurobiology, and several model organisms. We now know that homozygosity for ΔGAG has the same effects as Tor1aKO, implicating a partial loss of function mechanism in the ΔGAG/+ disease state. In addition, torsinA loss specifically affects neurons in mice, even though the gene is broadly expressed, apparently because of differential expression of homologous torsinB. Furthermore, certain neuronal subtypes are more severely affected by torsinA loss. Interestingly, these include striatal cholinergic interneurons that display abnormal responses to dopamine in several Tor1a animal models. There is also progress on understanding torsinA molecular cell biology. The structural basis of how ΔGAG inhibits torsinA ATPase activity is defined, although mutant torsinAΔGAG protein also displays some characteristics suggesting it contributes to dystonia by a gain‐of‐function mechanism. Furthermore, a consistent relationship is emerging between torsin dysfunction and membrane biology, including an evolutionarily conserved regulation of lipid metabolism. Considered together, these findings provide major advances toward understanding the molecular, cellular, and neurobiological pathologies of DYT1/TOR1A dystonia that can hopefully be exploited for new approaches to treat this disease. © 2016 International Parkinson and Movement Disorder Society
LRRK2 levels and phosphorylation in Parkinson's disease brain and cases with restricted Lewy bodies
Authors: Dzamko Nicolas , Gysbers Amanda M. , Bandopadhyay Rina , Bolliger Marc F. , Uchino Akiko , Zhao Ye , Takao Masaki , Wauters Sandrine , van de Berg Wilma D. J. , Takahashi‐Fujigasaki Junko , Nichols R. Jeremy , Holton Janice L. , Murayama Shigeo , Halliday Glenda M. 
Published: December 2016

ABSTRACTBackground: Leucine rich repeat kinase 2 (LRRK2) is a promising target for the treatment of Parkinson's disease; however, little is known about the expression of LRRK2 in human brain and if/how LRRK2 protein levels are altered in Parkinson's disease.Objectives: We measured the protein levels of LRRK2 as well as its phosphorylation on serines 910, 935, and 973 in the postmortem brain tissue of Parkinson's disease patients and aged controls with and without Lewy bodies.Methods: LRRK2 and its phosphorylation were measured by immunoblot in brain regions differentially affected in Parkinson's disease (n = 30) as well as subjects with Lewy bodies restricted to the periphery and lower brain stem (n = 25) and matched controls without pathology (n = 25).Results: LRRK2 levels were increased in cases with restricted Lewy bodies, with a 30% increase measured in the substantia nigra. In clinical Parkinson's disease, levels of LRRK2 negatively correlated to disease duration and were comparable with controls. LRRK2 phosphorylation, however, particularly at serine 935, was reduced with clinical Parkinson's disease with a 36% reduction measured in the substantia nigra.Conclusions: Our data show that LRRK2 phosphorylation is reduced with clinical PD, whereas LRRK2 expression is increased in early potential prodromal stages. These results contribute to a better understanding of the role of LRRK2 in idiopathic Parkinson's disease and may aid efforts aimed at therapeutically targeting the LRRK2 protein. © 2016 International Parkinson and Movement Disorder Society
Capillary Electrophoresis as Tool for Diastereomeric Separation in aTrichilia catiguaFraction
Authors: Sereia Ana Luiza , Longhini Renata , Lopes Gisely Cristiny , de Mello João Carlos Palazzo 
Published: December 2016

AbstractIntroductionThe treeTrichilia catigua, popularly known as “catuaba”, shows several biological activities and has emerged as a potential source of new drugs. Considering that more than 10 species are known under the same popular name, regulatory agencies require more rigorous quality control of this medicinal plant.ObjectiveTo develop and validate a methodology using capillary electrophoresis (CE) with ultraviolet (UV) detection for analysing polyphenols in the ethyl‐acetate fraction (EAF) ofTrichilia catigua.MethodologyDifferent electrophoretic conditions (such as wavelength of UV detection, voltage, buffer concentration and pH, cyclodextrin type and concentration) were investigated. After optimisation, borate buffer 80 mmol/L at pH 8.80 with 2‐hydroxypropyl‐β‐cyclodextrin 10 mmol/L was selected as background electrolyte. A voltage reduction was used to improve the separation of a diastereomeric pair of cinchonains.ResultsThe method proved to be simple, sensitive, accurate, linear, precise and reproducible. For the first time in natural products analysis, a voltage reduction and hydroxypropyl‐β‐cyclodextrin were used to improve the separation of diastereomeric pairs. Until now, this is the only described methodology able to separate catechin, epicatechin, cinchonains Ia, Ib, IIa, and IIb fromTrichilia catiguasamples on the same run in less than 12 min. When compared to the high performance liquid chromatography with photo‐diode array detection (HPLC‐PDA) method previously developed by our research group, the CE method was more efficient, faster, less expensive and less polluting.ConclusionOur results demonstrate that this method could be employed in a quality‐control laboratory for the quantification of polyphenols in EAF ofTrichilia catigua. Copyright © 2016 John Wiley & Sons, Ltd.
Separating and Identifying the Four Stereoisomers of Methyl Jasmonate by RP‐HPLC and using Cyclodextrins in a Novel Way
Authors: Matencio Adrián , Bermejo‐Gimeno Mario J. , García‐Carmona Francisco , López‐Nicolás José Manuel 
Published: December 2016

AbstractIntroductionSeveral authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed‐phase high performance liquid chromatography (RP‐HPLC) has been developed.Objective(1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection.Materials and Methods – RP‐HPLC using a 250 mm C18 column and different proportions of cyclodextrins (CDs) and organic solvents was applied to a commercial sample of racemic MeJA.ResultsThe results show that the best conditions for separating the MeJA stereoisomers are: 20% methanol in the mobile phase, a temperature of 45 °C and a 16 mM concentration of methyl‐β‐cyclodextrin (M‐β‐CD). A simple C18 250 mm column and a flow rate of 1.25 mL/min were used. The reduction in the retention time of MeJA observed when M‐β‐CD is added to the mobile phases was used to determine the complexation constants of the guest/CD complex and compared with the obtained when other CDs were used. The KF for M‐β‐CD (117.49 ± 5.9 1/M) was obtained with a 1:1 stoichiometry. The four stereoisomers were identified by molecular docking techniques and coinjection of a commercially available rosemary essential oil.ConclusionThe new method identified and classified the four stereoisomers of MeJA in the following ordination: (−)epiMeJA, (−)MeJA; (+)MeJA and (+)epiMeJA. These results could be used to improve the elicitation of cell cultures with only the best isomer. Copyright © 2016 John Wiley & Sons, Ltd.
Reverse Phase Compatible TLC‐Bioautography for Detection of Tyrosinase Inhibitors
Authors: García Paula , Ramallo I. Ayelen , Furlan Ricardo L.E. 
Published: December 2016

AbstractIntroductionReverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved.ObjectivesTo develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC‐plates that can be used for bioguided isolation of bioactive compounds.MethodsEnzyme gel entrapment with an amphiphilic copolymer was used for assay development. The gel turns into a brown “skin like” colour due to tyrosinase catalysed oxidation of l‐tyrosine. The inhibitors are visualised as clear spots against a brown coloured background.ResultsThe assay was able to localise cinnamaldehyde in Cinnamomum cassia essential oil, as its main constituent with known tyrosinase inhibition properties. The assay allowed the detection of 0.03% (w/w) of kojic acid co‐spotted with a methanolic extract of Sphaeralcea bonariensis and chromatographed on RP‐TLC.ConclusionThe developed assay is able to detect, with high sensitivity, tyrosinase inhibitors present in complex matrices that were chromatographed in RP‐TLC. Results can be easily read by colour change, inhibitors appear as clear spots in a darker background. Copyright © 2016 John Wiley & Sons, Ltd.
Octreotide use and safety in infants with hyperinsulinism
Authors: McMahon Ann W. , Wharton Gerold T. , Thornton Paul , De Leon Diva D. 
Published: December 2016

AbstractBackgroundOctreotide is a synthetic peptide analog of naturally occurring somatostatin. Octreotide is used off‐label in children <6 years of age for hyperinsulinism, chylothorax, and gastrointestinal bleeding. There is a lack of controlled data on efficacy or potential adverse events from this off‐label use.MethodsThree pediatric hospitals participated in this study. Patients were hospitalized January 2007–December 2010 and administered octreotide for congenital hyperinsulinism (CHI) at least 1 day. Variables assessed included octreotide dosage, patient demographics, medical interventions, concomitant medicines, serious adverse events (SAEs) including necrotizing enterocolitis (NEC), and mortality.ResultsThe 103 patient sample had a median gestational age of 38 weeks. During the study period, two patients died: one from NEC and the other from cardiomyopathy/sepsis. There were 11 other SAEs in the 101 surviving patients.ConclusionThis study highlights potential risks in administering octreotide off‐label. This study, like several other published studies, has highlighted NEC in a full‐term infant treated with octreotide. It is important to study the efficacy and the safety of octreotide for hyperinsulinism. In the interim, it might be prudent to prescribe octreotide in CHI neonates only in the absence of other risk factors for NEC. Copyright © 2016 John Wiley & Sons, Ltd.
The role of individual and personality factors in controlling risky behaviours related to AIDS: Proposing a causal model
Authors: Rezaei Mansour , Zakiei Ali , Reshadat Soheyla , Ghasemi Seyed Ramin 
Published: December 2016

AbstractBackgroundInvestigating previous studies show that personality traits have an important role in controlling risky behaviours related to AIDS; therefore, the aim of this study is to investigate the relationship between AIDS health literacy, personality traits and mental health and controlling risky behaviours related to AIDS through self‐efficacy.MethodsThe statistical population includes all the young people in western provinces of Iran, 2015. Data analysis was carried out for a sample of 756 participants (59% female).ResultsThe results show that except for the socializing trait, all the other variables are related to controlling risky behaviours. In addition, variables of health literacy related to AIDS, mental health, activity, impulsive sensation seeking and hostility have a direct relation to controlling risky behaviours. Also, the predicting behaviours can predict 62% of the variance in controlling risky behaviours related to AIDS. The analysis results show that health literacy has an indirect impact on controlling risky behaviours through self‐efficacy. In other words, health literacy related to AIDS leads to controlling risky behaviours when self‐efficacy is high for controlling risky behaviours.ConclusionBased on the results, it is recommended that the role of self‐efficacy in controlling risky behaviours be considered as a strategy for preventing AIDS. Copyright © 2016 John Wiley & Sons, Ltd.
Highly efficient organic photovoltaic cells fabricated by electrospray deposition using a non‐halogenated solution
Authors: Takahira Kazuya , Toda Asuki , Suzuki Katsumi , Fukuda Takeshi 
Published: December 2016

Since a large amount of solvents are needed for roll‐to‐roll printing processes of organic photovoltaic cells, an environmentally friendly process for practical mass production systems is in high demand. In this research, a highly efficient organic photovoltaic cell was successfully demonstrated by the electrospray deposition method using a non‐halogenated solvent, o‐xylene. An addition of acetonitrile and 1,8‐diiodooctane drastically reduced domain size of the organic active layer, resulting in improved device performance. In addition, the photoconversion efficiency was continuously increased with increasing the solvent evaporation time of a droplet deposited on a substrate, and the highest photoconversion efficiency of 5.6% was achieved by optimizing the solvent evaporation time.Current‐density–voltage characteristics under irradiation with AM 1.5 light and corresponding AFM images of the active layers electrosprayed with and without the addition of 1,8‐diiodooctane in o‐xylene and acetonitrile.
Bulky bis(imidazole) and 2‐sulfoterephthalate Assisted 3‐D Cu(II) and 2‐D Mn(II) Coordination Polymers: Topology, Diversity in Metal Containing Nodes and Spectral Elucidation
Authors: Das Kuheli , Datta Amitabha , Mane Sandeep B. , Mendiratta Shruti , Massera Chiara , Garribba Eugenio , Huang Jui‐Hsien , Hung Chen‐Hsiung , Akitsu Takashiro 
Published: December 2016

AbstractThis work presents the synthesis of a 3‐D Cu(II) framework represented by a 4,6‐c 2‐nodal net of the type fsc employing the bifunctional template group sulfonate‐carboxylate and the dia precursor (dia=9,10‐di(1H‐imidazol‐1‐yl)anthracene). The high versatility of the N‐donor and carboxylate ligands also yields a 2‐D Mn chain described by a pcu primitive cubic net. The solvent‐dependent EPR studies for both the CPs are also illustrated. Variable temperature magnetic susceptibility confirms that both ferromagnetic and antiferromanetic interactions coexist in 1 among the different geometrical arrangement of Cu(II) ions.
Cu(OAc)2/I2‐Mediated Direct Sulfonylation of Benzo[d]imidazoles with Disulfides
Authors: Wei Liang , Deng Jian‐Chao , Zhuo Bao‐Liu , Xu Li , Tang Ri‐Yuan 
Published: December 2016

AbstractA novel method for direct sulfonylation of disulfides with benzo[d]imidazoles mediated by Cu(OAc)2 and iodine has been established. This protocol allows for the preparation of diverse sulfonamides with broad functionalities in moderate to good yields. The control experiments have shown that the oxygen atoms in sulfonamides come from water.
Quantitating the cell: turning images into numbers with ImageJ
Authors: Arena Ellen T , Rueden Curtis T , Hiner Mark C , Wang Shulei , Yuan Ming , Eliceiri Kevin W 
Published: December 2016

Modern biological research particularly in the fields of developmental and cell biology has been transformed by the rapid evolution of the light microscope. The light microscope, long a mainstay of the experimental biologist, is now used for a wide array of biological experimental scenarios and sample types. Much of the great developments in advanced biological imaging have been driven by the digital imaging revolution with powerful processors and algorithms. In particular, this combination of advanced imaging and computational analysis has resulted in the drive of the modern biologist to not only visually inspect dynamic phenomena, but to quantify the involved processes. This need to quantitate images has become a major thrust within the bioimaging community and requires extensible and accessible image processing routines with corresponding intuitive software packages. Novel algorithms both made specifically for light microscopy or adapted from other fields, such as astronomy, are available to biologists, but often in a form that is inaccessible for a number of reasons ranging from data input issues, usability and training concerns, and accessibility and output limitations. The biological community has responded to this need by developing open source software packages that are freely available and provide access to image processing routines. One of the most prominent is the open‐source image package ImageJ. In this review, we give an overview of prominent imaging processing approaches in ImageJ that we think are of particular interest for biological imaging and that illustrate the functionality of ImageJ and other open source image analysis software. WIREs Dev Biol 2017, 6:e260. doi: 10.1002/wdev.260This article is categorized under:

Early Embryonic Development > Development to the Basic Body Plan
Adult Stem Cells, Tissue Renewal, and Regeneration > Methods and Principles
Technologies > Analysis of Cell, Tissue, and Animal Phenotypes