bluuebunny/crossref_metadata_2025_split huggingface dataset:
PIK3CA regulates lactate transport through MCT1,MCT4 in diffuse large B-cell lymphoma
Authors: Zhao Yan , Li Junna , Yu jinwen , Wang Xiaoshu , Pang Xuelian , Ma Mingfu , Ma Zhiping , Zhang Wei , Cui Wenli 
Published: October 2024

Abstract

Background:As a metabolite of glycolysis, lactate can not only provide energy for tumor cells, but also participate in the occurrence and development of tumors, immune escape, metastasis and recurrence through a variety of mechanisms. However, little is known about the molecular mechanism of its transport regulation in diffuse large B-cell lymphoma （DLBCL） cells.
Objective:This study is designed to evaluate the expression of monocarboxylate transporters MCT1 and MCT4 in DLBCL and to investigate the mechanism by which PIK3CA regulates lactate transport via MCT1 and MCT4.
Methods:MCT1/4 protein was detected by immunohistochemistry.We used BYL-719 to selectively inhibit PIK3CA in DLBCL cell lines (NUDHL-1, OCI-LY8). Western blot was used to detect MCT1/4, c-myc, LDHA, PD-L1. Real-time quantitative PCR was used to detect MCT1/4 mRNA.
Results:MCT1/4 was highly expressed in non-GCB. When MCT1 was high,patient had poor sensitivity to the R-CHOP regimen.The expression of MCT4 was high in patients with IPI>0 and LDH>240 U/L.In vitro,after inhibiting PIK3CA, MCT1/4 were lower than the control, and PD-L1,c-myc,LDHA was lower than the blank group.The lactate,ROS in tumor cells increased,PH decreased.
Conclusion:MCT1/4 can be expressed in DLBCL, which is related to tumor classification and regulation of lactate transport.we speculate that by inhibiting PIK3CA, down-regulating MCT1/4 expression, regulating the process of lactate transport in DLBCL tumor cells, providing a new target for DLBCL treatment.
The redistributive effect of pensions. The case of Uruguay
Authors: Forteza Alvaro , Tuzman Diego 
Published: October 2024

Abstract

In this paper, we present estimations of the distribution and redistribution of per period income that can be associated to social security using two approaches, one that follows the conventional practice of treating pensions as government transfers and another one that proposes to measure pension income as the return of pension wealth. Using data for Uruguay, we find that the former approach estimates less inequality in the presence of pensions and much larger decrease in inequality due to pensions than the latter. We show that the implicit assumption that individuals would not increase voluntary savings in the absence of pensions contributes to a strong apparent equalizing effect of pensions. As several scholars have warned, this assumption is not warranted.
JEL Classification: D31, H55, I38
Enhanced Fault Detection and Isolation in Proton Exchange Membrane Fuel CellsUsing Modified Super Twisting Observer
Authors: Rahmani Asma , Bougrine Mohamed , Gazzam Noureddine , Benalia Atallah , Seghir Mohammed 
Published: October 2024

Abstract

This paper focuses on proton exchange membrane fuel cells (PEMFCs) and their air supply system. The study investigates the application of advanced observers, specifically higher-order sliding mode observers with PI injection, to precisely estimate the partial pressure of oxygen in PEMFC air supply systems. These observers continuously monitor real-time system behavior and detect abnormal conditions, including faults such as sudden air manifold leaks. The primary objective of this research is to enhance the reliability, durability, and efficiency of PEMFCs by detecting faults in their air supply system, which is crucial for achieving optimal performance.
Knowledge, Attitude and Practices about cross bracing protocol in ACL injury : A Survey on Physiotherapist
Authors: Shah Sarfraznawaz , Chauk Amruta , Kumar G. Palani , Joshi Rushikesh 
Published: October 2024

Abstract

Purpose: Anterior Cruciate Ligament injuries are the most commonly seen injuries in the athletes, not all of these injuries need to be operated and can be treated conservatively, while many of them choose surgical intervention there is no certainty of the person returning to pre injury level without proper rehabilitation. Here Cross-Bracing protocol plays an important role, which tends to heal the ACL without surgical intervention by usage of brace for a particular period of time while taking regular physiotherapy rehabilitation. There is a limited knowledge of the same among Physiotherapists, so our study aimed to check awareness about this protocol among the Physiotherapists.
Methods: A google form questionnaire was circulated among the Physiotherapists via mail and all social media platforms and data was collected for a period of 8 months. Calculated sample size was 80. Descriptive statistics, including frequencies and percentages, were used to analyze the survey responses.
Results: A total of 82 participants (34 males, 48 females), with ± 4 years of practice completed the survey, where varying levels of awareness about ACL injury management and cross bracing protocols was observed. With 16.9% incorporating the protocol in regular practice.
Conclusion: The survey highlights that physiotherapists recognize the value of managing ACL injuries and using cross bracing, giving desired outcomes. Despite its perceived benefits, only few physiotherapist consistently use cross bracing in treatment plans, indicating a need for further education on clinical guidelines and its long-term advantages, especially for high-risk groups.
Towards Mixed Reality-Navigated Surgery: Point Cloud Surface Registration for Automated 3D Image Overlay
Authors: Bonsmann Henrik , Vo An-Nhien , Ladikos Alexander , Kuetting Daniel , Schmitt Joachim , Arensmeyer Jan C , Feodorovici Philipp 
Published: October 2024

Abstract

Purpose
Minimally invasive and robotically assisted surgery relies on guidance systems to aid in navigation. Using high accuracy tracking, surface registration algorithms and extended reality (XR) devices, we aim to develop a system to provide a real-time, immersive visualization of previously acquired computed tomography (CT) data through automated surface registration as an intuitive and radiation free alternative to conventional image guiding methods.
Methods
Combining state-of-the art XR devices, high-precision tracking and scanning and advanced surface registration algorithms, we propose a modular and immersive system to provide a holographic overlay of relevant tools and anatomical structures in a surgical context.
We measure and verify the accuracy of various modalities to provide a suitable foundation for further technical enhancements and clinical studies.
Results
Accuracy measurements for the XR holographic overlay as well as automatically calculated positioning of CT data with surface registration are in line with conventional image-guided approaches.
Analysis of the acquired data indicates probable avenues to further optimize the system.
Conclusion
The system developed in this paper provides a promising foundation for an immersive, real-time guidance system for interventional, minimally invasive, and robotically assisted procedures.
Further studies to verify the accuracy and usability in a clinical context are initiated.
Unraveling the Ferroptosis-inducing Potential of Methanol Leaves Extract of Prosopis Juliflora Via Downregulation of SLC7A11 and GPX4 mRNA Expression in A549 Lung Cancer Cells
Authors: Alshammari Nawaf , Pandey Pratibha , Redhwan Alya , Bakhsh Hadeel R. , Lakhanpal Sorabh , Rab Safia Obaidur , Singh Ajay , Saeed Mohd , Khan Fahad , Shah Mohd Asif 
Published: October 2024

Introduction:
Prosopis juliflora has been employed in many traditional treatments.
As evidenced by our earlier research, Prosopis juliflora leaf methanol extract (PJME) has a
promising future in the fight against lung cancer. It may also be used in conjunction with
other treatments to effectively manage lung cancer.

Aims and Objective:
The main objective of this study was to explore the potential of PJME to
inhibit lung cancer in A549 cells, along with its underlying mechanisms of action.

Methods:
The antiproliferative effects were determined using MTT and LDH tests. Apoptosis-
inducing capacity was evaluated using the DAPI staining, caspase-3 test, cytochrome C
assay, PARP cleavage, and qRT-PCR. To investigate the mechanism of action of PJME in
lung cancer, the levels of ROS, MMP, GSH, MDA, and specific ferroptosis indicators were
measured.

Results:
The experimental data of the current study indicated that exposure of A549 cells to
PJME reduced cell viability and increased cellular cytotoxicity. The apoptosis-inducing ability
of PJME in A549 cells was validated by enhanced nuclear condensation, level of the caspase-
3, cytochrome C, and PARP release. In addition, qRT-PCR investigations verified that
the administration of PJME led to a decrease in the expression of anti-apoptotic gene Bcl2
while enhancing the mRNA level of pro-apoptotic genes, such as Bax and caspase-3, in
A549 cells.

Conclusion:
The study also found that PJME has the ability to activate ferroptosis pathways,
as evidenced by elevated reactive oxygen species (ROS) generation, changes in the
levels of antioxidant markers (MDA and GSH), and decreased expression of SLC7A11 and
GPX4. The results of the present study clearly showed that PJME inhibited the proliferation
of A549 cells and induced ferroptosis by reducing the expression of the important targets
SLC7A11 and GPX4. Further research is necessary to fully understand the clinical efficacy
of PJME before it can be investigated as supplemental or adjuvant therapy for lung cancer.
Establishment of an Integrated Population Pharmacokinetic/ Pharmacodynamics Model of Apixaban in Chinese Healthy Population Adjusting for Key Genetic Variants
Authors: Mu Guangyan , Liu Yaou , Xie Qiufen , Liu Zhiyan , Zhang Hanxu , Meng Xianmin , Song Jinfang , Wang Zhe , Zhou Shuang , Wang Zining , Hu Kun , Zhao Xia , Liao Maoxing , Bao Jiachun , Xiang Qian , Cui Yimin 
Published: October 2024

Aims:
To improve the understanding of pharmacokinetic/pharmacodynamic (PK/PD) profiles of
apixaban, supporting personalised drug prescriptions for future patients.

Background:
Genetic as well as nongenetic factors can affect the predictable PK and PD characteristics of
apixaban.

Objective:
Establish a integrated popPK/PD model that adjusts for critical genetic variant.

Methods:
The integrated PK/PD models was characterized on the basis of PK (apixaban blood concentration)
and PD (prothrombin time (PT), activated partial thromboplastin time (APTT), and anti-FXa activity) data
from 181 healthy Chinese volunteers. Other investigated covariate variables included: Meaningful intrinsic
and extraneous determinants, correlated genetic factors (ABCG2, F13A1, C3, etc.). A total of 2877 PK concentration
observations were included in the modeling dataset.

Results:
The PK model of apixaban is adopted by single compartment model with first-order oral absorption.
The estimated values of total clearance rate (CL/F), apparent distribution volume (V/F), and absorption rate
constant (KA) in the final model are 3.37 L/h, 28.2 L, and 0.781 1/h, respectively. The PK model includes significance
covariates such as FOOD, RBC, WT, and gene (ABCG2). The PD model of apixaban is adopted by
a linear direct effect model with additive error, which was used to describe the relationship between markers
such as APTT, PT, anti-FXa, versus plasma concentration. PK simulation within the modelled dose range is
similar to clinical real date, while PD simulation results also show that the simulated exposure parameters is
within the range of the literature.

Conclusion:
We established a comprehensive PK/PD model and used it to simulate markers level such as
APTT, PT, and anti-FXa of apixaban. Individual predictive values with a dose of 2.5 mg are basically within
the expected recommended range.
Advance Screening of Bis-azetidinone Derivatives: Synthesis, Spectroscopy, Antioxidant and Antimicrobial Analysis with Molecular Docking Assessment
Authors: Hussein Huda H. , Al-azawi Khalida F. , Hasoon Butheina A. , El-Sayed Doaa S. 
Published: October 2024

Introduction:
This study includes synthesis, characterizations, antimicrobial, antiox-idant, and docking molecular study of novel Bis-Azetidinone compounds that combined two units of β-lactam rings .In the present investigation, the aromatic aldehydes with primary amine were condensed to create Schiff's base, which was then reacted with chloroacetylchloride to pro-duce bis-Azetidinone compounds.

Methods:
Melting points, FTIR, and NMR spectrum analyses were used to examine the morphological and topological characteristics of the Bis-Azetidinone compounds. The results indicate that the prepared Compounds synthesis has excellent antimicrobial activity against both Gram-negative (Escherichia coli,), Gram-positive bacteria (Staphylococcus aureus) and fungal (Candida albicans) and also indicated that the Compounds synthesis (A2) gave a higher antimicrobial effect than the B2, C2. The synergistic activity was examined against the pathogenic microbial strains. It was observed that employing compound synthesis combined with antibiotics enhanced the synergistic efficacy compared to using compound synthesis alone or antibiotic alone on Gram-positive bacteria and fungi.

Results:
The antioxidant efficiency was assessed by DPPH, the results show that the compound synthesis has antioxidant activity, and also indicated that the synthesized compound (A2) gave a higher antioxidant effect than the B2, C2. Docking study confirmed via redocking of crystalized substrate or inhibitor within target binding pocket. The docking results reveal that the synthesized compounds, with a total binding affinity of less than -48 kcal/mol, could be clinically used for future therapeutic purposes.

Conclusion:
The present research demonstrates the advantageous effectiveness of a simpler production procedure, novel Bis-Azetidinone compounds, for producing high-purity with low hazard that may be utilized as future possible medical therapies.
Effectiveness of a Virtual Reality Serious Video Game (The Secret Trail of Moon) for Emotional Regulation in Children With Attention-Deficit/Hyperactivity Disorder: Randomized Clinical Trial
Authors: Martin-Moratinos Marina , Bella-Fernández Marcos , Rodrigo-Yanguas María , González-Tardón Carlos , Li Chao , Wang Ping , Royuela Ana , Lopez-Garcia Pilar , Blasco-Fontecilla Hilario 
Published: October 2024

Background
Difficulties in emotional regulation are often observed in children and adolescents with attention-deficit/hyperactivity disorder (ADHD). Innovative complementary treatments, such as video games and virtual reality, have become increasingly appealing to patients. The Secret Trail of Moon (MOON) is a serious video game developed by a multidisciplinary team featuring cognitive training exercises. In this second randomized clinical trial, we evaluated the impact of a 20-session treatment with MOON on emotional regulation, as measured by the Strengths and Difficulties Questionnaire.

Objective
We hypothesize that patients with ADHD using MOON will show improvements in (1) emotional regulation, (2) core ADHD symptoms, (3) cognitive functioning, and (4) academic performance, compared to a control group; additionally, we anticipate that (5) changing the platform (from face-to-face using virtual reality to the web) will not affect emotional regulation scores; and (6) the video game will not cause any clinically significant side effects.

Methods
This was a prospective, unicentric, randomized, unblinded, pre- and postintervention study with block-randomized sequence masking. Participants included individuals aged between 7 and 18 years who had a clinical diagnosis of ADHD and were receiving pharmacological treatment. They were randomized into 2 groups using an electronic case report form: the MOON group, receiving standard pharmacological treatment plus personalized cognitive training via a serious video game, and the control group, receiving standard pharmacological treatment. We provided both the groups with psychoeducational support on ADHD. Analysis was conducted using the Student 2-tailed t test and 2-factor ANOVA. An independent monitor supervised the study.

Results
A total of 76 patients with ADHD participated in the trial, with an equal randomization (MOON: n=38, 50% and control: n=38, 50%) and a total dropout rate of 7. The primary hypothesis, a 3- or 4-point reduction in the global Strengths and Difficulties Questionnaire score, was not met. However, significant improvements were observed in material organization (P=.03), working memory (P=.04), and inhibition (P=.05), particularly among patients more engaged with the MOON treatment.

Conclusions
Serious video games, when integrated into a multimodal treatment plan, can enhance outcomes for symptoms associated with ADHD.

Trial Registration
ClinicalTrials.gov NCT06006871; https://clinicaltrials.gov/study/NCT06006871

International Registered Report Identifier (IRRID)
RR2-10.2196/53191
Wearables and Smartphones for Tracking Modifiable Risk Factors in Metabolic Health: Protocol for a Scoping Review
Authors: Brügger Victoria , Kowatsch Tobias , Jovanova Mia 
Published: October 2024

Background
Metabolic diseases, such as cardiovascular diseases and diabetes, contribute significantly to global mortality and disability. Wearable devices and smartphones are increasingly used to track and manage modifiable risk factors associated with metabolic diseases. However, no established guidelines exist on how to derive meaningful signals from these devices, often hampering cross-study comparisons.

Objective
This study aims to systematically overview the current empirical literature on how wearables and smartphones are used to track modifiable (physiological and lifestyle) risk factors associated with metabolic diseases.

Methods
We will conduct a scoping review to overview how wearable and smartphone-based studies measure modifiable risk factors related to metabolic diseases. We will search 5 databases (Scopus, Web of Science, PubMed, Cochrane Central Register of Controlled Trials, and SPORTDiscus) from 2019 to 2024, with search terms related to wearables, smartphones, and modifiable risk factors associated with metabolic diseases. Eligible studies will use smartphones or wearables (worn on the wrist, finger, arm, hip, and chest) to track physiological or lifestyle factors related to metabolic diseases. We will follow the reporting guideline standards from PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews) and the JBI (Joanna Briggs Institute) guidance on scoping review methodology. Two reviewers will independently screen articles for inclusion and extract data using a standardized form. The findings will be synthesized and reported qualitatively and quantitatively.

Results
Data collection is expected to begin in November 2024; data analysis in the first quarter of 2025; and submission to a peer-reviewed journal by the second quarter of 2025. We expect to identify the degree to which wearable and smartphone-based studies track modifiable risk factors collectively (versus in isolation), and the consistency and variation in how modifiable risk factors are measured across existing studies.

Conclusions
Results are expected to inform more standardized guidelines on wearable and smartphone-based measurements, with the goal of aiding cross-study comparison. The final report is planned for submission to a peer-reviewed, indexed journal. This review is among the first to systematically overview the current landscape on how wearables and smartphones measure modifiable risk factors associated with metabolic diseases.

International Registered Report Identifier (IRRID)
PRR1-10.2196/59539
Perbandingan Disolusi Dispersi Padat Valsartan Dalam Bentuk Tablet dan Kapsul
Authors: Cahyani Sulastari , Laksitorini Marlyn Dian , Sulaiman Teuku Nanda 
Published: October 2024

ABSTRAK Valsartan adalah zat aktif yang sukar larut air yang mana kelarutannya bergantung pada pH saluran cerna. Dispersi padat merupakan salah satu metode yang efektif untuk meningkatkan kelarutan dan disolusi zat aktif sukar larut. Penelitian sebelumnya telah didapatkan dispersi padat valsartan dengan pembawa PVP VA, poloxamer 188 dan poloxamer 407 yang memiliki kelarutan jenuh dan disolusi intrinsik yang baik. Kendati demikian pelepasan dispersi padat valsartan dalam bentuk sediaan khususnya tablet dan kapsul belum diketahui, sebagaimana diketahui bahwa pembawa yang digunakan dan bentuk sediaan obat mempengaruhi profil disolusi. Penelitian ini bertujuan untuk membandingkan disolusi dispersi padat valsartan dalam bentuk sediaan tablet dan kapsul pada pH 1,2 dan 4,5. Karakteristik fisik tablet dispersi padat dispersi padat valsartan meliputi kekerasan, kerapuhan dan waktu hancur. Adanya PVP VA, poloxamer 188 dan poloxamer 407 pada sistem dispersi padat menghasilkan tablet yang memiliki pelepasan obat diperlama dan menghasilkan daya apung setelah diformulasi menjadi tablet. Kapsul dispersi padat valsartan memiliki profil disolusi yang lebih baik dibandingkan tablet dispersi padat valsartan pada pH 1,2 maupun pH 4,5. Nilai Q60 kapsul dispersi padat valsartan pada pH 1,2 dan 4,5 berturut-turut 2,73% dan 103,28% sedangkan nilai Q60 tablet dispersi padat valsartan berturut-turut 2,89% dan 36,75%.Kata Kunci : dispersi padat, valsartan, tablet, kapsul
THE EFFECT OF FUROSEMIDE IN CRITICALLY ILL ADULT PATIENTS – A NARRATIVE REVIEW
Authors: Putri Cyndi Yulanda , Nugroho Agung Endro , Widyati Widyati 
Published: October 2024

Background: Fluid overload is the common condition in the critically ill patients. This associated with the increased fluid intake and inadequate elimination. The management of fluid overload is by fluid removal with diuretic. Furosemide is a loop diuretic that frequently used as the initial therapy. However, the utilization of furosemide in the critically ill reminds a polarizing subject. The purpose of this study is to investigate the impact of furosemide on patients who are critically ill. Methods: The inclusion criteria included randomized controlled trials as well as observational cohort studies. The data sources utilized in this study were PubMed, Science Direct, ProQuest, and Cochrane.Results: We included 13 articles, of which 9 articles about generally critically ill patients with or without acute kidney injury (AKI), 2 articles about heart failure, and 2 articles about post operative. The furosemide was effective in generally critically ill patients with or without AKI, it can decrease the fluid balance, weight change, and improves the urine output. Furosemide had no harmful effect on kidney function. However, patients without oliguria were not recommended to receive high dose of furosemide. Critically ill patients with heart failure who received continuous infusion of furosemide were more susceptible to increased diuresis and greater depression of thoracic fluid content (TFC). Furthermore, it might cause the decrease of renal function. When compared to furosemide, continuous veno-venous hemodiafiltration (CVVHDF) was more successful at removing excess fluid, reducing weight, relieving symptoms, and improving hemodynamic and cardiac performance. In post operative patients, furosemide might cause metabolic alkalosis. Urinary electrolyte excretion rates were promptly altered by the use of low dose furosemide.Conclusion: Based on the patient's clinical data, furosemide use should be taken into consideration. In general, furosemide is effective to improve diuresis. Furosemide in the AKI condition with oliguria has beneficial effect. However, in heart failure condition, furosemide might affect renal function. In post operative, it might cause metabolic alkalosis. Further randomized controlled trial (RCT) is required.