bluuebunny/crossref_metadata_2025_split huggingface dataset:
SENSING SWEET SPOTS: A comprehensive study of SARS-CoV-2-binding lectins
Authors: Ramya T.N.C. , LNU Neetu , Ahlawat Shimona , Delipan Rathina , Ringe Rajesh , Rao Alka 
Published: September 2024

Abstract

The Spike glycoprotein of SARS-CoV-2 enables receptor binding, membrane fusion, and viral entry, and is the major target for vaccines and therapeutics. Spike is heavily glycosylated, and N-glycosylation is critical for the open conformation required for ACE2 binding. ACE2 binding and subsequent viral fusion are blocked by inhibitors of N-glycan biosynthesis and by a few lectins. Here, we performed a comprehensive study of lectins that bind to recombinant SARS-CoV-2 Spike glycoprotein (expressed and purified from HEK293F cells) and to SARS-CoV-2 virus (cultured from Vero E6 cells) by employing an array of 95 lectins with different carbohydrate specificities, 68 of which we characterized using publically available glycan array data and the MotifFinder software. Spike glycoprotein was bound by N-glycan core binding lectins, AMA, ASA, BANLEC, BC2LA, CA, ConA, GNA, GS-II, HHA, LcHA, MNA-M, NPA, ORYSATA, PHA-L, and TL, and O-glycan binding lectins, ACL, SBA, ABL, LSN-L, and RCA-60. Spike glycoprotein was also bound by L-fucose-specific lectins, PSA, RS-Fuc, and AAL (in an L-fucose-dependent manner). A subset of the N-glycan core binding lectins and other lectins that bound to recombinant Spike - AMA, ASA, ConA, GNA, NPA, BANLEC, and TL, and AAL, RCA-120, SBA, and PHA-P, respectively, also bound well to cultured SARS-CoV-2 virus. The lectins, CPA, MAA, PA-IIL, and STL bound with greater intensity to the cultured SARS-CoV-2 virus than the recombinant Spike glycoprotein, suggestive of more accessible complex glycans on the cultured virus. The SARS-CoV-2-specific lectins identified in our study may be assessed for their antiviral potential in future studies.
Phenotypic, Molecular Detection, and Antibiogram Analysis of Pseudomonas strain from Oreochromis Niloticus .L 1758 (Nile Tilapia) from aquaculture pond, Ethiopia
Authors: Abdi Alemu kebede , pavanasam NATARAJAN , Rufael Tesfaye , Senbata Bayeta , Mohamed Abde Aliy 
Published: September 2024

Abstract

Pseudomonas species (including P. aeruginosa, P. putida, and P. fluorescens) are zoonotic bacterial pathogens that frequently cause disease and significant mortality among both cultured and wild fish worldwide. In Ethiopia, Pseudomonas species have been identified in Sebeta fish ponds and Rift Valley lakes. However, information on the molecular and phenotypic characteristics of Pseudomonas species in Ethiopian aquaculture ponds is limited. To address this gap, a cross-sectional study was conducted from November 2022 to May 2023 in selected aquaculture ponds in Ethiopia. A total of 637 samples were aseptically collected from the muscle, liver, spleen, and kidney of fish in these ponds using purposive sampling methods. The samples were cultured on Pseudomonas base agar with the selective supplement cetriNix (FD029) media and glycerol and subjected to morphological and biochemical tests to isolate and identify Pseudomonas species. The pathogen was isolated from 81 samples, accounting for 12.7% of the total. Among these isolates, 85.6% exhibited virulence traits, such as β-hemolysis on blood agar with 5% sheep blood. Additionally, 75 strains (92.59%) were confirmed using conventional PCR with Pseudomonas-specific primers and an optimized protocol. Among the PCR-positive samples, 8 (10.66%) were identified as P. aeruginosa, 28 (37.63%) as P. putida, and 39 (52%) as P. fluorescens from Nile Tilapia (O. niloticus). Antibiotic susceptibility testing on ten representative isolates showed that all Pseudomonas isolates were susceptible to Ciprofloxacin, Gentamicin, and Ceftriaxone, but resistant to Amoxicillin and Penicillin. The study concludes that Pseudomonas species (including P. aeruginosa, P. putida, and P. fluorescens) strains carrying the virulence gene Psul, which are β-hemolytic and resistant to commonly used antibiotics in human and veterinary medicine, are present in Ethiopian aquaculture. The detection of this pathogen in 75 fish samples is concerning due to the potential for outbreaks and zoonotic transmission. Therefore, further research into the molecular epidemiology of the disease is needed to understand potential inter-host transmission and antibiotic resistance traits. Additionally, public awareness about the risks of consuming undercooked or raw fish meat should be raised.
The Association Between Family Rituals, Family Functioning, and Student Well-being
Among Asian Undergraduates
Authors: Perez Jeannie A. , Tauy Jhunar John M. , Gomes Cedila P. , Pitogo Eden Grace , Ynalvez Cherry V. 
Published: September 2024

Abstract

Family rituals and routines are associated with family dynamics and children’s well-being. However, no study examines the specific family ritual and routine settings related to family functioning and student well-being. This study explores the association of family rituals and routine settings- such as dinnertime, weekends, and annual celebrations—on family functioning and student well-being. Likewise, it investigates whether family functioning mediates the association between family rituals and student well-being. This non-experimental quantitative research involved 622 undergraduate students from Thailand, Vietnam, India, and the Philippines with 71 percent female, and 29 percent male participants. The participants completed an online survey that included the Family Ritual Questionnaire, McMaster Family Functioning Scale, and the Student Well-being Scale (Five-Factor). Path analysis was conducted using the PROCESS module within the SMART-PLS environment to examine the proposed model. Results indicate that weekends and annual celebrations are positively associated with overall student wellbeing, whereas dinnertime is not. However, dinnertime, weekends, and annual celebrations are positively associated with family functioning. Family functioning served as a mediating factor for the positive associations of all three settings on student well-being, controlling for the effects of gender and country differences. Additionally, the study found variations in well-being across countries and variations in family functioning by gender and by country. These results emphasize the importance of fostering and maintaining family rituals and routines to enhance overall family dynamics and student well-being within Asian contexts.
Enhancing groundwater resource detection in Ahmednagar District through an integrated GIS framework utilizing AHP, SI, and FR models
Authors: Dongare Pranit Nitin , Sharma Kul Vaibhav , Kumar Vijendra , Gautam Lilesh , Singh Karan 
Published: September 2024

ABSTRACT

Groundwater resources affected by climate change exacerbate groundwater level declines in areas relying on erratic monsoons and finite surface water supplies. This research developed and compared an integrated geographic information system (GIS) with the analytical hierarchy process (AHP), the statistical index model (SI), and the frequency ratio model (FR). Multiple layers of the GIS framework have captured surface and subsurface characteristics, including rainfall, slope, elevation, land use/land cover, soil, geology, and geomorphology. 30% of the 69 wells in the study area are the testing dataset, and the remaining 70% make up the training dataset. Based on AHP, the results show that 35.34% of the region has low potential, 43.47% has moderate potential, and 21.09% has high potential. While SI modeling reveals 9.60, 57.36, and 24.79% for low, moderate, and high potential, respectively, FR analysis suggests 25.50% low potential, 52.74% moderate potential, and 21.60% high potential areas. The superior reliability of the SI model has been indicated by the validation of the GWPZ map using receiver operating characteristic area under the curve values. The results of this study are crucial for planning sustainable water supplies and managing groundwater resources in areas susceptible to climate change and groundwater depletion.
Polycyclic aromatic hydrocarbons (PAHs) in crude oil-contaminated water and soil and their removal using locally available plant materials
Authors: Ayedun Hassan , Jaiyeola Oluyimika Omowoleola , Onigbinde Stephen Oluwapelumi , Folarin Olujimi Moses , Oyedeji Abdulrasaq Olalekan 
Published: September 2024

ABSTRACT

The presence of polycyclic aromatic hydrocarbons (PAHs) in water and soil can be harmful to human life when ingested. PAHs are determined in the water and soil of the B-Dere community of Rivers State, Nigeria. The concentration level, source identification, and treatment were carried out. The water samples were treated with garlic and Moringa seed extracts, while the soil samples were treated with garlic and Fenton oxidation reagents. PAHs were extracted before and after treatment. The gas chromatograph mass spectrometer analyses showed 13 PAHs in the water and 10 PAHs in the soil. The highest concentration in water was recorded for benzo(ghi)perylene, with a mean value of 27.7 ± 0.25 ngL−1, while that of soil was recorded for benz(a)anthracene, with a mean value of 14.4 ± 0.631 ngkg−1. The source of PAHs in water was pyrogenic, while that of soil was petrogenic. Garlic extract removed 100% of benz(a)anthracene and benzo(b)fluorothane from the water, while Moringa removed 100% biphenylene from the water. However, garlic extract removed 2.59% of acenaphthylene, while Fenton reagents removed 100% of anthracene, phenathrene, and chrysene from the soil. Moringa seed and garlic extracts can be used in PAH's polluted water treatment.
Expression Profile and Prognostic Values of IRX Family Members in Lung Adenocarcinoma
Authors: Zhang Jieli , Zhou Yanqiu , Li Xiaoli , Wang Shufang , Zhang Peng , Li Dongbing , Zhou Yunzhi 
Published: September 2024

Background:
The potential role of the iroquois homeobox (IRX) genes in tumorigenesis
is a subject of interest, yet their specific involvement in lung adenocarcinoma
(LUAD) has not been extensively examined.

Objective:
This research endeavored to explore the impact of the IRX genes on the onset
and progression of LUAD.

Methods:
Utilizing data from The Cancer Genome Atlas (TCGA), samples of LUAD
were selected for analysis. The influence of the IRX genes was scrutinized through various
tools, including Kaplan-Meier Plotter, cBioPortal, and the R programming language
(version 3.6.3), with gene expression levels being confirmed in cellular models via quantitative
real-time polymerase chain reaction (qRT-PCR).

Results:
It was observed that the levels of IRX1/2/3/6 were notably diminished in LUAD
tissues when juxtaposed with healthy lung tissue. Conversely, the expression of
IRX4 was found to be elevated in LUAD. Correlations were identified between IRX
gene expression and several clinical parameters such as T stage, smoking history quantified
in pack-years, lymph node involvement, patient gender, initial treatment responses,
and smoking status. The diminished expression of IRX2/5 emerged as a significant indicator
of an unfavorable prognosis in LUAD. The study also highlighted the potential of
various IRX genes as diagnostic indicators for LUAD. These genes were implicated in
the facilitation of LUAD's growth and spread through a range of biological pathways, encompassing
the Ras signaling and more. Additionally, a pronounced link was discovered
between the infiltration of immune cells and the expression patterns of the IRX genes.
IRX genes were abnormally expressed in LUAD cell lines.

Conclusion:
IRX gene family could serve not only as indicators of LUAD prognosis but
also as therapeutic targets for LUAD.
DRP1 Association in Inflammation and Metastasis: A Review
Authors: Nandan Parmar Keshri , Job Anica Tholath , Ramasamy Tamizhselvi 
Published: September 2024

:
In recent years, mitochondria have gained significant interest in the field of biomedical
research due to their impact on aging, human health, and other advanced findings in metabolic
functions. The latest finding shows that metabolic interventions are a leading cause of several diseases,
which has sparked interest in finding new therapeutic treatments. Apart from this, the
unique inheritance of genetic material from mother to offspring can help scientists find ways to
prevent mitochondrial inherited diseases. Additionally, the anti-aging benefits of controlling mitochondrial
functions are also being researched. The present study aims to provide a cohesive
overview of the latest findings in mitochondrial research, focusing on the role of DRP1 (Dynamin-
related protein 1), a member of the GTPase family, in mediating mitochondrial fission. The
first section of this paper provides a concise explanation of how DRP1 controls processes such as
mitophagy and mitochondrial fission. Subsequently, the paper delves into the topic of inflammation,
discussing the current findings regarding the inflammatory response mediated by DRP1. Finally,
the role of mitochondrial fission mediated by DRP1 in cancer is examined, reviewing ongoing
research on various types of cancer and their recurrence. Moreover, this review also covers the
epigenetic regulation of mitochondrial fission. The studies were selected, and evaluated, and the
information was collected to present an overview of the key findings. By exploring various aspects
of research and potential links, we hope to contribute to a deeper understanding of the intricate
relationship between the fields of cancer research and inflammation studies with respect to mitochondrial-
based research.
Laparoscopic ureteroplasty of a transplanted kidney using a healthy native ureter
Authors: Guliev B. G. , Komyakov B. K. , Avazkhanov Zh. P. , Abdurakhmanov O. Sh. 
Published: September 2024

Introduction. Ureteral strictures are a common urological complication of kidney transplantation. For short narrowings of the ureter, endoscopic operations are used; for extended defects the Boari operation is often performed. An alternative method may be to use a native ureter.Purpose of the study. To study the results of such operations in four patients.Materials & methods. We observed four patients with extended ureteral strictures of a transplanted kidney. Among them were three women and one man. Previously, all patients had undergone a cadaveric kidney transplant; the time from the operation itself to the development of stricture ranged from 3 months to 13 years. Initially, all patients underwent percutaneous drainage of the pyelocaliceal system of the transplanted kidney. After stabilization of creatinine values, the pelvis or pyeloureteral segment of the transplanted kidney was isolated using a transperitoneal approach. Further, the lower third of the ipsilateral native ureter was crossed at the level of the iliac vessels. Its upper end was clipped and anastomosis with the pelvis or ureteropelvic junction of the graft was performed.Results. The procedures were successful in all patients. In three patients the operation was completed using a laparoscopic approach. In one patient in whom extensive ureteral obliteration developed 3 months after transplantation against the background of incompetent ureterocystoneoastomosis and urinary leakage, when the pelvis was isolated, which was covered by the external iliac vein, the latter was injured with bleeding. This required conversion to open access, suturing of the iliac vein defect, further excision of the pelvis and anastomosis with the native ureter. In the post-operative period, the patient developed thrombosis of the iliac and femoral veins below the suturing area, and successful thrombolytic therapy was performed. Nephrostomy drain was removed before discharge and the stent was removed on an outpatient visit 4 to 6 weeks after surgery. Currently the condition of all patients is stable, the graft is functioning, and their diuresis is unchanged, serum creatinine ranges from 106 to 180 μmol/l.Conclusion. The use of a healthy native ureter is an adequate method of replacement of extended ureteral strictures of the transplanted kidney.
Androgen receptor gene CAG-trinucleotide repeat length affects function of endothelium in men with hypogonadism and type 2 diabetes mellitus
Authors: Khripun I. A. , Ismailov R. S. , Belousov I. I. , Ibishev Kh. S. , Kogan M. I. 
Published: September 2024

Introduction. The influence of the length of the number of CAG repeats in the androgen receptor gene (nCAG AR) on endothelial dysfunction (EnD) is currently understudied.Objective. The study aimed to evaluate the relationship between the nCAG AR and the dynamics of biochemical and ultrasound markers of EnD in men with functional hypogonadism and type 2 diabetes mellitus (T2DM) receiving testosterone replacement therapy (TRT).Materials & methods. This study included 45 hypogonadal men with T2DM, receiving TRT for 1 year. Patients were assessed for carbohydrate and lipid metabolism parameters; total and free T; sex hormone-binding globulin; biochemical markers of EnD (NO, eNOS3, endothelin) and the nCAG AR; brachial artery (BA) vasoreactivity. Patients were divided into 3 groups: group I — 9 men with nCAG AR < 19; group II — 27 men with nCAG AR > 19 – 24; and group III — 9 men with nCAG AR >24.Results. Patients with nCAG AR < 19 exhibited a 2-fold greater and faster increase in BA vasoreactivity on TRT compared to patients with nCAG AR 19-24 and 3-fold greater than men with nCAG AR >24 (p < 0.05). Patients with nCAG AR < 19 also demonstrated the most pronounced rise in NO and eNOS3 on TRT compared to men with nCAG AR > 24. Patients with nCAG AR < 19 experienced the most pronounced decreases in weight, waist circumference, and HbA1c on TRT compared to other patients (p < 0.05).Conclusion. The nCAG AR length significantly affects the response to TRT in men with hypogonadism and T2DM. The most significant improvements are seen in patients with short nCAG AR.
Transurethral balloon dilation safety and efficacy in patients with recurrent bladder neck contracture after transurethral interventions
Authors: Abbosov Sh. A. , Shomarufov A. B. , Aminova D. R. 
Published: September 2024

Introduction. Currently, urologists are divided on which method is most effective for treating bladder neck contracture (BNC) after surgery: transurethral resection (TUR) or incision.Objective. The study aimed to assess the balloon dilation safety and success in patients with recurrent bladder neck contracture (BNC) after endoscopic surgery for benign prostatic hyperplasia (BPH).Materials & Methods. This study involved 110 patients with recurrent BNC after transurethral interventions for BPH. All patients underwent TUR of the bladder neck and were divided into 2 groups: group A (n = 45) included men who, after TUR, additionally underwent repeated balloon dilation, while the control group B (n = 65) included men who were treated with alpha-blockers alone.Results. In 9 months after TUR and 3 months after the fourth balloon dilation procedure in group A, the mean international prostate symptom score (IPSS) decreased from 20.1 ± 8.4 to 17.2 ± 7.4, and the IPSS-quality of life (QoL) was 4.2 ± 1.2 (p > 0.05). In group B, they were 21.7 ± 7.7 and 4.7 ± 1.1 (p > 0.05), respectively. Additionally, the mean flow rate in group A was 13.2 ± 5.4 ml/s, while in group B, it was 8.7 ± 4.9 ml/s (p < 0.05). There was a significant decrease in the post-void residual urine volume (PVR) from 76.2 ± 96.1 ml to 37.6 ± 55.1 ml in group A, whereas, in group B, it increased from 63.0 ± 36.9 ml to 79.4 ± 71.6 ml (p > 0.05). Also, 28.0% of patients of group B, and 13.3% of patients of group A underwent repeated TUR of the bladder neck in 9 months follow-up period (p < 0.05).Conclusion. Balloon dilation procedure is a relatively safe less invasive procedure and can reduce the BNC recurrence rate and consequently the rate of repeated transurethral interventions.
Design and Implementation of an Opioid Scorecard for Hospital System–Wide Peer Comparison of Opioid Prescribing Habits: Observational Study
Authors: Slovis Benjamin Heritier , Huang Soonyip , McArthur Melanie , Martino Cara , Beers Tasia , Labella Meghan , Riggio Jeffrey M , Pribitkin Edmund deAzevedo 
Published: September 2024

Abstract

Background
Reductions in opioid prescribing by health care providers can lead to a decreased risk of opioid dependence in patients. Peer comparison has been demonstrated to impact providers’ prescribing habits, though its effect on opioid prescribing has predominantly been studied in the emergency department setting.

Objective
The purpose of this study is to describe the development of an enterprise-wide opioid scorecard, the architecture of its implementation, and plans for future research on its effects.

Methods
Using data generated by the author’s enterprise vendor–based electronic health record, the enterprise analytics software, and expertise from a dedicated group of informaticists, physicians, and analysts, the authors developed an opioid scorecard that was released on a quarterly basis via email to all opioid prescribers at our institution. These scorecards compare providers’ opioid prescribing habits on the basis of established metrics to those of their peers within their specialty throughout the enterprise.

Results
At the time of this study’s completion, 2034 providers have received at least 1 scorecard over a 5-quarter period ending in September 2021. Poisson regression demonstrated a 1.6% quarterly reduction in opioid prescribing, and chi-square analysis demonstrated pre-post reductions in the proportion of prescriptions longer than 5 days’ duration and a morphine equivalent daily dose of >50.

Conclusions
To our knowledge, this is the first peer comparison effort with high-quality evidence-based metrics of this scale published in the literature. By sharing this process for designing the metrics and the process of distribution, the authors hope to influence other health systems to attempt to curb the opioid pandemic through peer comparison. Future research examining the effects of this intervention could demonstrate significant reductions in opioid prescribing, thus potentially reducing the progression of individual patients to opioid use disorder and the associated increased risk of morbidity and mortality.