bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spondyloarthritis and Tietze’s syndrome: A re-evaluation
Authors: Matsuki Yasunori , Nakamura Tadashi 
Published: September 2024

ABSTRACT
Spondyloarthritis (SpA) comprises a number of related but different disorders with distinct phenotypes: psoriatic arthritis, reactive arthritis, arthritis related to inflammatory bowel disease, undifferentiated arthritis, and ankylosing spondylitis (the well-known prototypic subtype). Differentiating rheumatic diseases, such as rheumatoid arthritis, synovitis-acne-pustulosis-hyperostosis-osteitis syndrome, pustulotic arthro-osteitis, gout, and SpA, is difficult because they all may manifest swelling at the upper anterior chest wall, often involve the sternocostal and/or sternoclavicular joints, and clearly show cutaneous nodular symptoms, so that they may mimic Tietze’s syndrome in the presentation. Tietze’s syndrome is a benign, self-limiting entity with tender, non-suppurative swelling in the upper anterior chest wall, especially at the second and third costosternal junctions and the sternoclavicular joint. Therefore, distinguishing SpA from Tietze’s syndrome during an entire disease course is important, even after an initial tentative diagnosis. This article aims to re-evaluate the importance of Tietze’s syndrome in the differential diagnosis of SpA, while keeping in mind information about rheumatic diseases affecting the upper anterior chest wall.
RTP801 interacts with the tRNA ligase complex and dysregulates its RNA ligase activity in Alzheimer’s disease
Authors: Campoy-Campos Genís , Solana-Balaguer Julia , Guisado-Corcoll Anna , Chicote-González Almudena , Garcia-Segura Pol , Pérez-Sisqués Leticia , Torres Adrian Gabriel , Canal Mercè , Molina-Porcel Laura , Fernández-Irigoyen Joaquín , Santamaria Enrique , de Pouplana Lluís Ribas , Alberch Jordi , Martí Eulàlia , Giralt Albert , Pérez-Navarro Esther , Malagelada Cristina 
Published: September 2024

Abstract
RTP801/REDD1 is a stress-responsive protein overexpressed in neurodegenerative diseases such as Alzheimer’s disease (AD) that contributes to cognitive deficits and neuroinflammation. Here, we found that RTP801 interacts with HSPC117, DDX1 and CGI-99, three members of the tRNA ligase complex (tRNA-LC), which ligates the excised exons of intron-containing tRNAs and the mRNA exons of the transcription factor XBP1 during the unfolded protein response (UPR). We also found that RTP801 modulates the mRNA ligase activity of the complex in vitro since RTP801 knockdown promoted XBP1 splicing and the expression of its transcriptional target, SEC24D. Conversely, RTP801 overexpression inhibited the splicing of XBP1. Similarly, in human AD postmortem hippocampal samples, where RTP801 is upregulated, we found that XBP1 splicing was dramatically decreased. In the 5xFAD mouse model of AD, silencing RTP801 expression in hippocampal neurons promoted Xbp1 splicing and prevented the accumulation of intron-containing pre-tRNAs. Finally, the tRNA-enriched fraction obtained from 5xFAD mice promoted abnormal dendritic arborization in cultured hippocampal neurons, and RTP801 silencing in the source neurons prevented this phenotype. Altogether, these results show that elevated RTP801 impairs RNA processing in vitro and in vivo in the context of AD and suggest that RTP801 inhibition could be a promising therapeutic approach.
PerturbDB for unraveling gene functions and regulatory networks
Authors: Yang Bing , Zhang Man , Shi Yanmei , Zheng Bing-Qi , Shi Chuanping , Lu Daning , Yang Zhi-Zhi , Dong Yi-Ming , Zhu Liwen , Ma Xingyu , Zhang Jingyuan , He Jiehua , Zhang Yin , Hu Kaishun , Lin Haoming , Liao Jian-You , Yin Dong 
Published: September 2024

Abstract
Perturb-Seq combines CRISPR (clustered regularly interspaced short palindromic repeats)-based genetic screens with single-cell RNA sequencing readouts for high-content phenotypic screens. Despite the rapid accumulation of Perturb-Seq datasets, there remains a lack of a user-friendly platform for their efficient reuse. Here, we developed PerturbDB (http://research.gzsys.org.cn/perturbdb), a platform to help users unveil gene functions using Perturb-Seq datasets. PerturbDB hosts 66 Perturb-Seq datasets, which encompass 4 518 521 single-cell transcriptomes derived from the knockdown of 10 194 genes across 19 different cell lines. All datasets were uniformly processed using the Mixscape algorithm. Genes were clustered by their perturbed transcriptomic phenotypes derived from Perturb-Seq data, resulting in 421 gene clusters, 157 of which were stable across different cellular contexts. Through integrating chemically perturbed transcriptomes with Perturb-Seq data, we identified 552 potential inhibitors targeting 1409 genes, including an mammalian target of rapamycin (mTOR) signaling inhibitor, retinol, which was experimentally verified. Moreover, we developed a ‘Cancer’ module to facilitate the understanding of the regulatory role of genes in cancer using Perturb-Seq data. An interactive web interface has also been developed, enabling users to visualize, analyze and download all the comprehensive datasets available in PerturbDB. PerturbDB will greatly drive gene functional studies and enhance our understanding of the regulatory roles of genes in diseases such as cancer.
Will ChatGPT-4 improve the quality of medical abstracts?
Authors: Gravel Jocelyn , Dion Chloé , Fadaei Kermani Mandana , Mousseau Sarah , Osmanlliu Esli 
Published: September 2024

Abstract

Background
ChatGPT received attention for medical writing. Our objective was to evaluate whether ChatGPT 4.0 could improve the quality of abstracts submitted to a medical conference by clinical researchers.

Methods
This was an experimental study involving 24 international researchers (the participants) who provided one original abstract intended for submission at the 2024 Pediatric Academic Society (PAS) conference. We asked ChatGPT-4 to improve the quality of the abstract while adhering to PAS submission guidelines. Participants received the revised version and were tasked with creating a final abstract. The quality of each version (original, ChatGPT and final) was evaluated by the participants themselves using a numeric scale (0–100). Additionally, three co-investigators assessed abstracts blinded to the version. The primary analysis focused on the mean difference in scores between the final and original abstracts.

Results
Abstract quality varied between the three versions with mean scores of 82, 65 and 90 for the original, ChatGPT and final versions, respectively. Overall, the final version displayed significantly improved quality compared to the original (mean difference 8.0 points; 95% CI: 5.6–10.3). Independent ratings by the co-investigators confirmed statistically significant improvements (mean difference 1.10 points; 95% CI: 0.54–1.66). Participants identified minor (n = 10) and major (n = 3) factual errors in ChatGPT’s abstracts.

Conclusion
ChatGPT 4.0 does not produce abstracts of better quality than the one crafted by researchers but it offers suggestions to help them improve their abstracts. It may be more useful for researchers encountering challenges in abstract generation due to limited experience or language barriers.
A DUF21 domain–containing protein regulates plant dwarfing in watermelon
Authors: Sun Piaoyun , Zhao Hongjiao , Cao Lihong , Zhang Tian , Zhang Helong , Yang Tongwen , Zhao Bosi , Jiang Yanxin , Dong Junyang , Chen Tianrui , Jiang Biao , Li Zheng , Shen Junjun 
Published: September 2024

Abstract
Dwarf or semidwarf plant structures are well suited for intensive farming, maximizing yield, and minimizing labor costs. Watermelon (Citrullus lanatus) is classified as an annual vine plant with elongated internodes, yet the mechanism governing watermelon dwarfing remains unclear. In this study, a compact watermelon mutant dwarf, induced by the insertion of transferred DNA (T-DNA), was discovered. Through resequencing, a gene named domain of unknown function 21 (ClDUF21), located downstream of the T-DNA insertion site, was identified as the candidate gene for the dwarf mutant, and its functionality was subsequently confirmed. Watermelon mutants generated through CRISPR/Cas9-mediated knockout of ClDUF21 revealed that homozygous mutants displayed a pronounced dwarfing phenotype, and protein–protein interaction analysis confirmed the direct interaction between ClDUF21 and ClDWF1. Subsequently, we employed CRISPR/Cas9 technology to precisely modify the homologous gene CsDUF21 in cucumber (Cucumis sativus) and performed protein interaction validation between CsDUF21 and CsDWF1, thereby demonstrating that the CsDUF21 gene also exhibits analogous functionality in plant dwarfing. These findings demonstrate that ClDUF21 governs plant dwarfism by modulating the brassinosteroid synthesis pathway via ClDWF1.
Case-based peer teaching for pain medicine fellows: a curriculum for diverse residency backgrounds
Authors: Chai Nu Cindy , Brar Nikhraj , Su Po-Yi Paul 
Published: September 2024

Abstract

Introduction
Pain medicine is an advanced medical subspecialty incorporating trainees from diverse primary disciplines. A curriculum that does not assess or promote the trainees’ differential expertise developed from their primary specialties may silo instead of promoting collaborative learning in a multidisciplinary subspecialty.

Methods
We created a Kern-guided curriculum for the pain medicine fellowship utilizing case-based modules and a novel note-taking and knowledge-sharing format. Nine faculty-led, case-based modules of increasing complexity were developed based on pain medicine competency content. Pain medicine fellows were instructed to actively designate prior and new knowledge into 4 categories: prior knowledge that is accurate and relevant, inaccurate prior knowledge, new knowledge gained from peers, and new knowledge for all trainees. After each case study, each fellow acknowledged “new knowledge gained from peers” and prepared a brief teach-back of “new knowledge for all trainees” at the beginning of the next session. Fellow participants were assessed for their reactions to the curriculum via anonymized surveys.

Results
The curriculum was implemented at a single institution pain medicine fellowship from 2021 to 2023. At the end of this curriculum, 90% of participants reported improved knowledge retention through participating in this case-based curriculum compared to the traditional didactic format, and 80% of participants reported increased respect for their peers.

Discussion
We created a novel note-taking and knowledge-sharing platform, anchored by a well-established case-based educational strategy, to improve the learning environment and knowledge retention for multidisciplinary trainees with heterogeneous baseline knowledge.
Death-associated protein kinase 3 modulates migration and invasion of triple-negative breast cancer cells
Authors: Wang Junkai , Tran-Huynh Anh M , Kim Beom-Jun , Chan Doug W , Holt Matthew V , Fandino Diana , Yu Xin , Qi Xiaoli , Wang Jin , Zhang Weijie , Wu Yi-Hsuan , Anurag Meenakshi , Zhang Xiang H F , Zhang Bing , Cheng Chonghui , Foulds Charles E , Ellis Matthew J 
Published: September 2024

Abstract
Sixteen patient-derived xenografts (PDXs) were analyzed using a mass spectrometry (MS)-based kinase inhibitor pull-down assay (KIPA), leading to the observation that death-associated protein kinase 3 (DAPK3) is significantly and specifically overexpressed in the triple-negative breast cancer (TNBC) models. Validation studies confirmed enrichment of DAPK3 protein, in both TNBC cell lines and tumors, independent of mRNA levels. Genomic knockout of DAPK3 in TNBC cell lines inhibited in vitro migration and invasion, along with down-regulation of an epithelial–mesenchymal transition (EMT) signature, which was confirmed in vivo. The kinase and leucine-zipper domains within DAPK3 were shown by a mutational analysis to be essential for functionality. Notably, DAPK3 was found to inhibit the levels of desmoplakin (DSP), a crucial component of the desmosome complex, thereby explaining the observed migration and invasion effects. Further exploration with immunoprecipitation–mass spectrometry (IP–MS) identified that leucine-zipper protein 1 (LUZP1) is a preferential binding partner of DAPK3. LUZP1 engages in a leucine-zipper domain–mediated interaction that protects DAPK3 from proteasomal degradation. Thus, the DAPK3/LUZP1 heterodimer emerges as a newly discovered regulator of EMT/desmosome components that promote TNBC cell migration.
Ultrasonography-led multimodal diagnostic pathway for giant cell arteritis
Authors: Mukhtyar Chetan B , Beadsmoore Clare , Ducker Georgina , Fordham Sarah , Sisson Katherine , Jones Colin 
Published: September 2024

AbstractObjectivesThis study aims to establish the sensitivity and negative predictive value of a multimodal pathway incorporating ultrasonography, 18-fluorodeoxyglucose labelled PET-CT and temporal artery biopsy for the diagnosis of giant cell arteritis.MethodsIn total, 1000 consecutive referrals for a new diagnosis of giant cell arteritis were analysed. All patients had a protocolized examination. Patients with a negative ultrasonography and a CRP of ≥20 mg/L received an extended ultrasound examination. If that was negative, and there was no other explanation for their presentation, a second test in the form of either a temporal artery biopsy or an 18-fluorodeoxyglucose labelled PET-CT was offered. We calculated the sensitivity and negative predictive value of the interventions for diagnosing giant cell arteritis.Results279/1000 patients had positive ultrasonography for giant cell arteritis. 202 had bilateral superficial temporal arterial involvement. Ultrasonography of the axillary artery and other head/neck arteries increased the yield by 53 and 24 patients, respectively. 181 patients were referred for a second test. 24/139 temporal artery biopsies and 7/42 18-fluorodeoxyglucose labelled PET-CT scans were positive. The sensitivity and negative predictive value rise from 62.3% and 84.7%, respectively, for imaging superficial temporal arteries alone, to 95.7% and 98.0%, respectively, for extended ultrasonography plus a second test.ConclusionThis is the first real-world evidence of the utility of ultrasonography for the diagnosis of giant cell arteritis as part of a multimodal diagnostic pathway.
Associations of Primary Spoken Language With Individual Perception of Hearing-Related Disability
Authors: Bell Rebecca K. , Dillard Lauren K. , McRackan Theodore R. , Zhan Kevin Y. , Dubno Judy R. , Saunders James E. , Dixon Peter R. 
Published: September 2024

Objectives:
Hispanic/Latino adults are less likely than non-Hispanic White adults to seek treatment for hearing disability. While differential socioeconomic factors may contribute to this finding, differences in phonology and syntax in the Spanish, versus English, language may also influence patient perception of hearing disability. The objective of this study is to investigate the association between primary language spoken and participant perception of hearing disability.

Design:
This study represents a cross-sectional cohort study using National Health and Nutrition Examination Study cycles 2015–2016 and 2017–2020 data. Multivariable logistic regressions estimated the association between respondent-selected interview language, which was used as a proxy for primary spoken language, and participant perception of hearing disability. Models were adjusted for age, gender, highest degree of education, pure-tone average, and self-reported general health. Participants included 4687 individuals from the United States population who elected to speak English (n = 4083) or Spanish (n = 604) during the interview. Perception of hearing disability was assessed by (1) frequency of reported difficulty in following a conversation in noise, (2) frequency with which hearing caused respondents to experience frustration when talking with members of their family or friends, and (3) participants’ subjective overall assessment of their hearing.

Results:
Speaking Spanish, versus English, as a primary language was associated with a 42% lower odds of reporting difficulty hearing and understanding in background noise (odds ratio [OR]: 0.58, 95% confidence interval [CI]: 0.48 to 0.70). Spanish speakers had 28% lower odds of reporting feeling frustrated when talking to family members or friends due to hearing (OR: 0.72, 95% CI: 0.59 to 0.88) as compared with the English-speaking cohort. Speaking Spanish additionally conferred 31% lower odds of describing their own general hearing as “a little trouble to deaf” than participants speaking English (OR: 0.69, 95% CI: 0.53 to 0.90). These observed associations were independent of age, gender, highest degree of education, better pure-tone average? and self-reported general health.

Conclusions:
Primary Spanish speakers may be less likely than English speakers to report hearing-related disability, an effect which may be independent of ethnicity. Patient perception of hearing-related disability is an important component of the assessment of and counseling for hearing-related disability and discussion of the need for amplification or other hearing intervention.
Comparative Analysis of Taper Point and Reverse Cutting Needles on Skin Puncture Force
Authors: Maloney McKenzie E. , Potter Christian T. , Chun Benjamin D. , Montilla Richard D. , Schanbacher Carl F. 
Published: September 2024

BACKGROUND
Reverse cutting needles are commonly used in cutaneous surgery due to their perceived ease of use. Despite this, there is limited research evaluating the force required to puncture skin using contemporary needles.

OBJECTIVE
This study aims to compare the puncture forces required for two different needle geometries across various gauge sizes.

MATERIALS AND METHODS
The authors assessed the force necessary to penetrate samples of human abdominal skin samples using taper needles of three different United States Pharmacopeia gauge sizes with their respective reverse cutting needle counterparts. Taper point needles tested were RB-1 (3-0), TF (4-0), and C-1 (5-0), while reverse cutting needles included PS-2 (3-0) and P-3 (4-0, 5-0). An electronic force meter was used to record the puncture force required by each needle type.

RESULTS
The mean puncture force in newtons (N) for taper point needles was 1.00, 0.74, and 0.48 for RB-1, TF, and C-1, respectively. The mean puncture force for reverse cutting needles was 0.95 N, 0.60 N, and 0.51 N for PS-2, P-3 (4-0), and P-3 (5-0), respectively. There was a direct relationship between needle body diameter and puncture force for both needle geometries.

CONCLUSION
There was no clinically significant difference in skin puncture force between needle geometries.
A Systematic Review of Questionnaires Assessing Patient Satisfaction in Plastic Surgery: Tools, Topics, and Surgical Types
Authors: Gelidan Adnan G. , Al Qurashi Abdullah A. , Dahlawi Maryam , Hafiz Bayan F. , Halawani Ibrahim R. , Mandora Roaa M. , Tariq Shahad , Hennawi Yasser B. , Bukhari Rahaf I. , Alobaidi Hussain Amin 
Published: September 2024

Background:
Patient satisfaction is crucial for evaluating healthcare services, including plastic surgery. This systematic review aims to analyze questionnaires assessing patient satisfaction in plastic surgery, identifying their strengths and weaknesses to improve outcomes and enhance the quality of care.

Methods:
A comprehensive literature search was conducted using electronic databases. Studies were included if they were original research articles, written in English, and focused on patient satisfaction questionnaires in plastic surgery. Data extraction and descriptive statistics were used to summarize the data.

Results:
A total of 105 studies were included. General/overall satisfaction was the most common topic addressed (99.04%). Cosmetic outcomes were the most frequently assessed category (34.3%). Breast reconstruction was the most common procedure (33.3%). Most studies used a combination of generic and procedure-specific questionnaires (45.71%). The most frequently used measurement tools were BREAST-Q and self-developed questionnaires, each accounting for 28.57% and 27.61%.

Conclusions:
This review provides a comprehensive analysis of patient satisfaction questionnaires in plastic surgery, emphasizing the importance of a holistic approach and well-established, validated tools. The findings contribute to improving plastic surgery outcomes and enhancing the quality of care. Future research should refine assessment tools to address patients’ needs and promote patient-centered outcomes in plastic surgery.
Optimal Technique for Cutting Peripheral Nerves in Nerve Transfer Surgery: A Survey of Peripheral Nerve Surgeons
Authors: Catapano Joseph , Wong Chloe R. , Sarkhosh Tara , Stefaniuk Stephanie , Dengler Jana 
Published: September 2024

Background:
Nerve transfer procedures are performed in patients with proximal nerve injuries to optimize their potential for functional recovery. The study aimed to determine the preferred surgical technique and tool used by peripheral nerve surgeons to transect nerves in nerve transfers.

Methods:
All current members of the American Society of Peripheral Nerve were invited to complete a cross-sectional 10-question survey. Data on practice demographics, nerve-cutting instruments/techniques used, and their belief on whether this impacted patient outcomes were collected.

Results:
A total of 49 American Society of Peripheral Nerve members participated in the study, the majority of whom were over 10 years into practice (n = 30/49; 61%). The most common response was a scalpel blade (n = 26/49; 53%), with the remaining 47% using iris scissors, micro-serrated scissors, a razor blade, specialized nerve microscissors, or a specialized nerve-cutting device. The number of years in practice (P = 0.0271) and the percentage of practice that involves treating patients with peripheral nerve injuries (P = 0.0054) is significantly associated with the belief that crushing the donor nerves during transection may result in worse outcomes following nerve transfer. Only the latter is significantly associated with this belief in recipient nerves (P = 0.0214).

Conclusions:
Our findings demonstrate that peripheral nerve surgeons believe that the technique used to transect nerves before coaptation influences outcomes after nerve transfer. Further ex vivo studies are necessary to investigate how different cutting techniques influence nerve morphology and scarring at the coaptation site to optimize outcomes after peripheral nerve surgery.