bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in use of acute and preventive medications for migraine after erenumab initiation over 12 months: A United States retrospective cohort study
Authors: Multani Jasjit K. , Urman Robert , Park Andrew S. , Gill Karminder , Vuvu Fiston , Sun Kainan , Patel Leah B. , Stockl Karen M. , Hawkins Kevin , Rhyne Christopher , Bensink Mark E. 
Published: September 2024

AbstractObjectiveTo assess changes in real‐world use of acute and preventive medications for migraine over a 12‐month follow‐up period in the United States following initiation of the anti‐calcitonin gene‐related peptide (CGRP) pathway monoclonal antibody (mAb) erenumab.BackgroundEarly assessments of real‐world use of acute and preventive medications for migraine after initiation of erenumab have been limited to 6 months of follow‐up.MethodsThis retrospective cohort study used data from the IQVIA open‐source longitudinal prescription (LRx) and medical (Dx) claims databases. Adult patients with an initial claim (index date) for erenumab between May 2018 and April 2020 were identified.ResultsAmong 201,176 patients who met inclusion criteria, the mean (standard deviation [SD]) age was 47.5 (13.8) years and 85.6% (n = 172,153) were female. Most patients used one or more acute (88.4%; n = 177,795) and one or more traditional preventive (86.1%; n = 173,225) medications during the 12‐month pre‐index period. Adherence to erenumab (proportion of days covered [PDC] ≥0.80) was 40.2% (n = 80,927) with an overall mean (SD) PDC of 0.60 (0.34). Among all patients, 70.0% (n = 140,809) discontinued erenumab. After accounting for 24.7% (n = 49,720) of patients who restarted erenumab, discontinuation without reinitiation was observed in 45.3% (n = 91,089) of total patients. Switching to a different anti‐CGRP pathway mAb was observed in 13.1% (n = 26,446) of total patients. Among 177,795 patients with pre‐index use of one or more acute migraine medication class, 86.5% (n = 153,788) had post‐index use of the same class, and 56.7% (87,134/153,788) of them discontinued one or more class of acute medication in the 12‐month follow‐up period. Similarly, among 173,225 patients with pre‐index use of one or more traditional migraine preventive medication class, 67.7% (n = 117,274) had post‐index use of the same class, and 46.7% (54,790/117,274) of them discontinued one or more class of traditional preventive medication in the 12‐month follow‐up period.ConclusionsIn this long‐term study, we observed the discontinuation of both acute and preventive medications for migraine post‐erenumab initiation.
Impact of consumer familiarity on acceptability and purchase intent of a novel amaranth‐based coffee creamer
Authors: Sánchez‐Arellano Lucía , Ramírez‐Rivera Emmanuel de Jesús , López‐Espindola Mirna , Argumedo‐Macías Adrián , Cabal‐Prieto Adán , Juárez‐Barrientos José Manuel , Herrera‐Corredor José Andrés 
Published: September 2024

SummaryThe objective of the study was to identify the impact of consumer familiarity on liking, acceptability, purchase intention and textual description of a novel cream substitute formulated with popped amaranth flour. Four formulations were evaluated in different proportions of amaranth vs. a control. A total of 400 consumers from two regions participated (Veracruz: familiar with coffee and Tlaxcala: familiar with amaranth). Consumers from both regions had significant differences in liking the attributes of the different formulations. Liking across regions was mainly a function of product aroma. Regarding the acceptance of the product, it was found that the attributes flavour, colour and overall liking were significant (P < 0.05) for consumers from Veracruz, whereas appearance and mouthfeel were significant (P < 0.05) for consumers from Tlaxcala. In purchase intent, overall liking was significant for both regions (P < 0.05). In relation to the impact of amaranth on purchase intention, Tlaxcala showed higher purchase willingness. The words used for sensory characterisation and that are important for both regions were the amaranth aroma, and the creamy flavour in the formulation with the highest amaranth content. The findings of this study allowed for understanding the potential of amaranth to formulate new creamers in a cross‐cultural context, considering consumers from two different levels of familiarity with specific crops.
Clinical perspectives: Takotsubo cardiomyopathy
Authors: Ravindran Jayant , Brieger David 
Published: September 2024

AbstractTakotsubo syndrome is a condition characterised by temporary acute left ventricular dysfunction with regional wall abnormalities extending beyond a single coronary artery territory. Initially thought to be benign, this condition, which is challenging to distinguish from acute coronary syndrome, has substantial morbidity and mortality. The mechanism behind this condition remains elusive, but multiple theories have been proposed. Although beta blockers and angiotensin‐converting enzyme inhibitors are used as treatments for left ventricular dysfunction, currently, there are no randomised controlled trials to support their use. In this paper, we review the latest evidence regarding aetiologies, pathophysiology, diagnostic criteria, prognosis, complications and management of Takotsubo syndrome.
Skills Development in Hydrologic Sciences for Cohorts of Graduate Students from Morocco, Egypt, Türkiye, and Indonesia
Authors: Fryar Alan E. , Milewski Adam M. , Agouridis Carmen T. , Hanley Carol D. , Schroeder Paul A. , Sultan Mohamed , Ward James W. , Laftouhi Nour‐Eddine , Pandjaitan Nora H. , El Kadiri Racha , Benaabidate Lahcen , Fekri Ahmed , Suharyanto Agus , Yilmaz Koray K. 
Published: September 2024

AbstractIn developing countries in Africa and Asia, meeting challenges of water scarcity and pollution has often been hampered by shortcomings in higher education, including insufficient research productivity and funding, lack of opportunity for university graduates, and a mismatch between university activities and societal needs. To address these issues, we developed novel programs integrating technical instruction and preparation for professional practice in hydrology for cohorts of graduate students from Morocco and Egypt (2012–2013) and from Türkiye and Indonesia (2013–2014). Students participated in an initial online course and a follow‐up workshop featuring geographic information systems (GIS), remote sensing, and hydrologic modeling with internet‐based data sets. Field activities in the USA (first cohort) and in the students’ home countries (second cohort) included stream gauging, measurement of water levels in wells, water sampling, and measurement of hydrochemical parameters. A subsequent online course focused on research ethics, preparing proposals and publications, and presenting findings to technical audiences and the public, culminating in presentations at conferences in the USA. Participants mentored other students at their home institutions and at K‐12 schools in Türkiye and Indonesia. Participant feedback during and after the programs tended to be strongly positive, and participants have continued to engage with project leaders and mentor students in their home countries and the USA. Our modular, hybrid approach offers a template for students in hydrology and related fields to develop relevant skills and engage internationally.
Involvement of heparanase in the pathogenesis of acute pancreatitis: Implication of novel therapeutic approaches
Authors: Hamo‐Giladi Dalit B. , Fokra Ahmad , Sabo Edmond , Kabala Aviva , Minkov Irena , Hamoud Shadi , Hadad Salim , Abassi Zaid , Khamaysi Iyad 
Published: September 2024

AbstractAcute pancreatitis (AP) is a common gastrointestinal disease with high morbidity and mortality rate. Unfortunately, neither the etiology nor the pathophysiology of AP are fully understood and causal treatment options are not available. Recently we demonstrated that heparanase (Hpa) is adversely involved in the pathogenesis of AP and inhibition of this enzyme ameliorates the manifestation of the disease. Moreover, a pioneer study demonstrated that Aspirin has partial inhibitory effect on Hpa. Another compound, which possesses a mild pancreato‐protective effect against AP, is Trehalose, a common disaccharide. We hypothesized that combination of Aspirin, Trehalose, PG545 (Pixatimod) and SST0001 (Roneparstat), specific inhibitors of Hpa, may exert pancreato‐protective effect better than each drug alone. Thus, the current study examines the pancreato‐protective effects of Aspirin, Trehalose, PG545 and SST0001 in experimental model of AP induced by cerulein in wild‐type (WT) and Hpa over‐expressing (Hpa‐Tg) mice. Cerulein‐induced AP in WT mice was associated with significant rises in the serum levels of lipase (X4) and amylase (X3) with enhancement of pancreatic edema index, inflammatory response, and autophagy. Responses to cerulein were all more profound in Hpa‐Tg mice versus WT mice, evident by X7 and X5 folds increase in lipase and amylase levels, respectively. Treatment with Aspirin or Trehalose alone and even more so in combination with PG545 or SST0001 were highly effective, restoring the serum level of lipase back to the basal level. Importantly, a novel newly synthesized compound termed Aspirlose effectively ameliorated the pathogenesis of AP as a single agent. Collectively, the results strongly indicate that targeting Hpa by using anti‐Hpa drug combinations constitute a novel therapy for this common orphan disease.
A common variant in PIK3CG gene associated with the prognosis of heart failure
Authors: Hu Dong , Xiao Lei , Li Shiyang 
Published: September 2024

AbstractPhosphoinositide 3‐kinase γ (PI3Kγ) is G‐protein‐coupled receptor‐activated lipid kinase with both kinase‐dependent and kinase‐independent activity. Plenty of evidence have demonstrated that PI3Kγ participated in TAC and I/R‐induced myocardial remodelling and heart failure (HF). In this study, we tested the hypothesis that common variants in the PI3Kγ gene (PIK3CG) were associated with the prognosis of HF in the Chinese Han population. Through re‐sequencing and genotyping, we finally identified a common variant in the 3′UTR of PIK3CG strongly associated with the prognosis of HF in two‐stage population: adjusted p = 0.007, hazard ratio = 0.56 (0.36–0.85) in the first cohort and adjusted p = 0.024, hazard ratio = 0.39 (0.17–0.88) in the replicated cohort. A series of functional assays revealed that rs10215499‐A allele suppressed PIK3CG translation by facilitating has‐miR‐133a‐3p binding, but not the G allele. Subjects carrying the GG genotype showed higher mRNA and protein level than those with AA and AG genotype. Furthermore, overexpression of PIK3CG could protect AC16 from hypoxia/reoxygenation (H/R)‐induced apoptosis, while the case was opposite for PIK3CG silencing. In conclusion, common variant rs10215499 in the 3′‐UTR of PIK3CG might affect the prognosis of HF by interfering with miR‐133a‐3p binding and PIK3CG is a promising target for HF treatment in the future.
Increased forebrain EAAT3 expression confers resilience to chronic stress
Authors: Ardiles Nicolás M. , Tapia‐Cuevas Vissente , Estay Sebastián F. , Alcaino Alejandro , Velásquez Victoria B. , Sotomayor‐Zárate Ramón , Chávez Andrés E. , Moya Pablo R. 
Published: September 2024

AbstractDepression is a disabling and highly prevalent psychiatric illness. Multiple studies have linked glutamatergic dysfunction with the pathophysiology of depression, but the exact alterations in the glutamatergic system that contribute to depressive‐like behaviors are not fully understood. Recent evidence suggests that a decreased level in neuronal glutamate transporter (EAAT3), known to control glutamate levels and limit the activation of glutamate receptors at synaptic sites, may contribute to the manifestation of a depressive phenotype. Here, we tested the possibility that increased EAAT3 expression at excitatory synapses could reduce the susceptibility of mice to develop depressive‐like behaviors when challenged to a 5‐week unpredictable chronic mild stress (UCMS) protocol. Mice overexpressing EAAT3 in the forebrain (EAAT3glo/CMKII) and control littermates (EAAT3glo) were assessed for depressive‐like behaviors and long‐term memory performance after being subjected to UCMS conditions. We found that, after UCMS, EAAT3glo/CMKII mice did not exhibit depressive‐like behaviors or memory alterations observed in control mice. Moreover, we found that EAAT3glo/CMKII mice did not show alterations in phasic dopamine release in the nucleus accumbens neither in long‐term synaptic plasticity in the CA1 region of the hippocampus after UCMS, as observed in control littermates. Altogether these results suggest that forebrain EAAT3 overexpression may be related to a resilient phenotype, both at behavioral and functional level, to the deleterious effect of chronic stress, highlighting the importance of neuronal EAAT3 in the pathophysiology of depressive‐like behaviors.
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Clinical and genetic features of CMT2T in Italian patients confirm the importance of MME pathogenic variants in idiopathic, late‐onset axonal neuropathies
Authors: Geroldi Alessandro , La Barbera Andrea , Mammi Alessia , Origone Paola , Gaudio Andrea , Ponti Clarissa , Sanguineri Francesca , Matà Sabrina , Sperti Martina , Carboni Ilaria , Bellone Emilia , Gotta Fabio , Gemelli Chiara , Massucco Sara , Valeria Guglielmino , Marinelli Lucio , Grandis Marina , Bisogni Giulia , Sabatelli Mario , Piscosquito Giuseppe , Esposito Gabriella , Schenone Angelo , Manganelli Fiore , Mandich Paola , Tozza Stefano , Luigetti Marco 
Published: September 2024

AbstractBackground and AimsSince 2016, biallelic mutations in the membrane metalloendopeptidase (MME) gene have been associated with late‐onset recessive CMT2 (CMT2T). More recently, heterozygous mutations have also been identified in familial and sporadic patients with late‐onset axonal neuropathy, ranging from subclinical to severe. This indicates that the heterozygous MME variants may not be fully penetrant, or alternatively, that they may be a potential risk factor for neuropathy. Here, we describe the clinical, neurophysiological, and genetic findings of 32 CM2T Italian patients.MethodsThe patients were recruited from four different Italian referral centers. Following a comprehensive battery of neurological, electrophysiological, and laboratory examinations, the patients' DNA was subjected to sequencing in order to identify any variants in the gene. Bioinformatic and modeling analyses were performed to evaluate the identified variants' effects.ResultsWe observe a relatively mild axonal sensory‐motor neuropathy with a greater impairment of the lower extremities. Biallelic and monoallelic patients exhibit comparable disease severity, with an earlier onset observed in those with biallelic variants. When considering a subgroup with more than 10 years of disease, it becomes evident that biallelic patients exhibit a more severe form of neuropathy. This suggests that they are more prone to quick progression.InterpretationCM2T has been definitively defined as a late‐onset neuropathy, with a typical onset in the fifth to sixth decades of life and a more rapidly progressing worsening for biallelic patients. CMT2T can be included in the neuropathies of the elderly, particularly if MME variants heterozygous patients are included.
Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods
Authors: Gluhovic Vladimir , Timm Sara , Kuebler Wolfgang M. , Lopez‐Rodriguez Elena , Ochs Matthias 
Published: September 2024

AbstractThe alveolar surface of the lung is lined by an epithelium consisting of type I (AECI) and type II alveolar epithelial cells (AECII). This epithelium is covered by a liquid alveolar lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains the alveolar epithelial glycocalyx on the apical side of AECI and AECII. To better understand the alveolar epithelial glycocalyx, its ultrastructural visualization by transmission electron microscopy is required. The aim of this study was to systematically re‐evaluate routine cytochemical methods for visualization of the alveolar epithelial glycocalyx and specifically its glycan components. For this purpose, we used chemical fixation by vascular perfusion with aldehydes as a common routine approach in mice. After fixation, staining is needed for glycocalyx visualization. Cytochemical staining agents such as alcian blue, ruthenium red, and lanthanum nitrate were compared. In addition, SNL (Sambucus nigra lectin) and UEA1 (Ulex europaeus agglutinin I) were used for sialic acid and fucose‐specific labeling. Alcian blue showed the strongest staining, with cloud‐like structures, whereas ruthenium red appeared as thread‐like structures. On the other hand, lanthanum nitrate did not stain the alveolar epithelial glycocalyx. For specific sialic acid and fucose labeling, both lectins presented a specific signal. In conclusion, these methods can be used routinely for assessing ultrastructural changes of the alveolar epithelial glycocalyx in experimental in vivo models under different physiological and pathological conditions. In addition, cytochemical staining by tissue massage and post‐embedding lectin labeling after vascular perfusion support 3R (reduction, refinement, replacement) principles of animal welfare.
Poly‐d,l‐Lactic Acid Via Transdermal Microjet Drug Delivery for Treating Rosacea in Asian Patients
Authors: Seo Suk Bae , Wan Jovian , Thulesen Jesper , Jalali Arash , Vitale Massimo , Kim Soo‐Bin , Yi Kyu‐Ho 
Published: September 2024

ABSTRACTBackgroundRosacea, a chronic inflammatory skin condition, is marked by enduring redness, visible blood vessels, and inflammatory eruptions in facial areas. Managing rosacea remains a persistent challenge for dermatologists, especially in cases unresponsive to conventional treatments. Injectable poly‐d,l‐lactic acid (PDLLA) has shown promise in treating erythema and telangiectasia associated with rosacea in addition to age‐related concerns. Employing Mirajet, a laser‐induced microjet system, for administering PDLLA is a novel and promising treatment for rosacea.AimsWe aimed to evaluate the efficacy and safety of injectable PDLLA delivered via a needle‐free microjet system for managing rosacea.MethodsFour Korean women with persistent and refractory rosacea received five monthly sessions of PDLLA needle‐free injections. Clinical assessments were conducted using the Clinician's Erythema Assessment and Patient's Self‐Assessment (PSA) at baseline, 4 weeks post‐treatment, and 22 weeks post‐final treatment. Adverse events were monitored throughout the study period.ResultsAt 4 weeks post‐treatment, both Clinician's Erythema Assessment and PSA scores indicated significant improvements in erythema that were sustained up to the 22‐week follow‐up. Patients reported high satisfaction with resolution of redness and improved skin texture. Mild swelling, redness, and petechiae were observed post‐treatment but resolved spontaneously. No product‐related adverse events were noted during the study period.ConclusionInjectable PDLLA delivered via laser‐induced microjet injection demonstrated promising efficacy in improving rosacea symptoms and skin quality for up to 22 weeks without significant adverse effects. Larger randomized controlled trials are needed to confirm these findings and evaluate long‐term safety and sustainability of outcomes.
Portal vein thrombosis and hepatic infarction due to hepatic mobilization after primary debulking surgery for advanced ovarian cancer: A case report
Authors: Onishi Junki , Odajima Suguru , Koike Yuki , Takenaka Shin , Tanabe Hiroshi 
Published: September 2024

AbstractHepatic mobilization is essential in debulking surgery for resecting diaphragmatic lesions in advanced ovarian cancer. However, hepatic mobilization potentially induces postoperative portal vein thrombosis and hepatic infarction. No reports exist regarding these postoperative complications of gynecological surgeries. Thus, we reported a case of portal vein thrombosis and hepatic infarction after ovarian cancer surgery with upper abdominal surgery. The 51‐year‐old female patient who had been diagnosed with advanced ovarian and early endometrial cancer underwent primary debulking surgery. Ultimately, she underwent the following surgical procedures: a hysterectomy, bilateral salpingo‐oophorectomy, total parietal peritonectomy, low anterior resection, ileostomy, and appendicectomy. The hepatic enzymatic and D‐dimer levels were elevated, postoperatively. Contrast‐enhanced computed tomography revealed portal vein thrombosis and an infarction of the hepatic S3 region. The portal vein thrombosis resolved post‐administration of unfractionated heparin. The hepatic infarction improved. Meticulous intra‐ and postoperative management should encompass the deliberation of the potential risk of these postoperative complications.
Getting Down to Business: Governing the Hybridization of UK Charities
Authors: Curran Kevin , Ozcan Pinar 
Published: September 2024

AbstractCharity organizations are important to solving complex social and environmental issues that are beyond the reach of government and commercial organizations. However, these organizations are under increasing pressure for survival due to a sharp decrease in their traditional sources of funding. This study examines how leaders of charity organizations can improve the financial security and impact of their organization by adopting commercial structures into their organization, and therefore undergoing a process of hybridization. We conducted a multiple comparative case study of 18 UK charities comparing how they engaged with emerging social finance funding opportunities that required them to adopt commercial structures which lay outside their dominant logic of action. We identified several aspects that influenced the likelihood of a charity organization to engage with this opportunity and, therefore, strategically hybridize. These included whether a charity executive had sufficient socialization in both the social and commercial logics to view social finance as a strategic opportunity and whether the organization could alter the role expectations of trustees with a commercial background to enable them to actively use both logics rather than compartmentalizing them in their decision‐making. Our findings have important implications for research streams on hybridization and hybrid governance.