bluuebunny/crossref_metadata_2025_split huggingface dataset:
Of buds and bits: a meta-QTL study identifies stable QTL for berry quality and yield traits in cranberry mapping populations (Vaccinium macrocarpon Ait.)
Authors: Maule Andrew F. , Loarca Jenyne , Diaz-Garcia Luis , Lopez-Moreno Hector , Johnson-Cicalese Jennifer , Vorsa Nicholi , Iorizzo Massimo , Neyhart Jeffrey L. , Zalapa Juan E. 
Published: September 2024

IntroductionFor nearly two centuries, cranberry (Vaccinium macrocarpon Ait.) breeders have improved fruit quality and yield by selecting traits on fruiting stems, termed “reproductive uprights.” Crop improvement is accelerating rapidly in contemporary breeding programs due to modern genetic tools and high-throughput phenotyping methods, improving selection efficiency and accuracy.MethodsWe conducted genotypic evaluation on 29 primary traits encompassing fruit quality, yield, and chemical composition in two full-sib cranberry breeding populations—CNJ02 (n = 168) and CNJ04 (n = 67)—over 3 years. Genetic characterization was further performed on 11 secondary traits derived from these primary traits.ResultsFor CNJ02, 170 major quantitative trait loci (QTL; R2≥ 0.10) were found with interval mapping, 150 major QTL were found with model mapping, and 9 QTL were found to be stable across multiple years. In CNJ04, 69 major QTL were found with interval mapping, 81 major QTL were found with model mapping, and 4 QTL were found to be stable across multiple years. Meta-QTL represent stable genomic regions consistent across multiple years, populations, studies, or traits. Seven multi-trait meta-QTL were found in CNJ02, one in CNJ04, and one in the combined analysis of both populations. A total of 22 meta-QTL were identified in cross-study, cross-population analysis using digital traits for berry shape and size (8 meta-QTL), digital images for berry color (2 meta-QTL), and three-study cross-analysis (12 meta-QTL).DiscussionTogether, these meta-QTL anchor high-throughput fruit quality phenotyping techniques to traditional phenotyping methods, validating state-of-the-art methods in cranberry phenotyping that will improve breeding accuracy, efficiency, and genetic gain in this globally significant fruit crop.
Resistance to anti-smoking messages related to the higher smoking stigma of Korean female smokers
Authors: Ha Seung-Hyuk , Lee Gi-Eun , Hwang Jang-Sun , Lee Jang-Han 
Published: September 2024

IntroductionThe degree of perceived smoking stigma can differ, based on various factors such as gender; this may influence the effect of smoking cessation interventions, including denormalization. This study investigates the gender differences in smoking stigma recognized by Korean smokers and explores the effect of these differences on the success of smoking cessation messages that aim to initiate an identity crisis among smokers. It aims to contribute to effective smoking cessation intervention strategies for female smokers.MethodsThe smoker-gender Implicit Association Test (IAT) was used to measure gender-based smoking stigma; the test comprised photos of people smoking, with positive and negative descriptors. Participants were 120 smokers aged 19–35 years (60 males and 60 females). Participants’ cognitive attitudes toward smoking and cessation intentions were assessed at baseline. To investigate the effect of social stigmatization on smokers, participants were asked to watch anti-smoking campaigns that stigmatized either smoking behavior or smokers’ self-identity. Cognitive attitudes and cessations intention were used to show differences in gender and message conditions.ResultsThe IAT D-score showed that female smokers perceived other female smokers significantly more negatively than they did male smokers, suggesting a higher level of smoking stigma. Female smokers in the socially stigmatizing condition reduced their negativity toward smoking less than those who were not stigmatized. Moreover, cessation intentions did not improve when female smokers received identity-threatening messages, indicating that female smokers tended to resist stigmatizing messages.DiscussionsThese findings provide empirical evidence that the gender of Korean smokers is significantly related to differences in smoking stigma. The negative perception and resistance responses of female smokers shown in this study are consistent with the findings of previous studies on the stigma of substance use disorders and addiction. High smoking stigma can also be a risk factor in anti-smoking interventions, including health communication; therefore, these findings should be interpreted with caution.
Assessing the nonlinear impact of green space exposure on psychological stress perception using machine learning and street view images
Authors: Zhang Tianlin , Wang Lei , Zhang Yazhuo , Hu Yike , Zhang Wenzheng 
Published: September 2024

IntroductionUrban green space (GS) exposure is recognized as a nature-based strategy for addressing urban challenges. However, the stress relieving effects and mechanisms of GS exposure are yet to be fully explored. The development of machine learning and street view images offers a method for large-scale measurement and precise empirical analysis.MethodsThis study focuses on the central area of Shanghai, examining the complex effects of GS exposure on psychological stress perception. By constructing a multidimensional psychological stress perception scale and integrating machine learning algorithms with extensive street view images data, we successfully developed a framework for measuring urban stress perception. Using the scores from the psychological stress perception scale provided by volunteers as labeled data, we predicted the psychological stress perception in Shanghai's central urban area through the Support Vector Machine (SVM) algorithm. Additionally, this study employed the interpretable machine learning model eXtreme Gradient Boosting (XGBoost) algorithm to reveal the nonlinear relationship between GS exposure and residents' psychological stress.ResultsResults indicate that the GS exposure in central Shanghai is generally low, with significant spatial heterogeneity. GS exposure has a positive impact on reducing residents' psychological stress. However, this effect has a threshold; when GS exposure exceeds 0.35, its impact on stress perception gradually diminishes.DiscussionWe recommend combining the threshold of stress perception with GS exposure to identify urban spaces, thereby guiding precise strategies for enhancing GS. This research not only demonstrates the complex mitigating effect of GS exposure on psychological stress perception but also emphasizes the importance of considering the “dose-effect” of it in urban planning and construction. Based on open-source data, the framework and methods developed in this study have the potential to be applied in different urban environments, thus providing more comprehensive support for future urban planning.
Psychological burden of hearing-impaired children and their parents through the COVID-19 pandemic
Authors: Li Donglei , Lin Ken , Cen Xinru , Fan Yuwei , Hong Liping , Wu Zhao , Chen Wenliang , Zhong Xinqi 
Published: September 2024

ObjectiveChildhood hearing impairment has potential repercussions on the mental well-being of both children and their parents. As a vulnerable population in accessing health care services, they may face specific challenges, especially during the COVID-19 pandemic. This cross-sectional study aims to investigate the association between childhood hearing impairment and the mental health of children and their parents, and to assess health care utilization of hearing-impaired children and its impact on mental outcomes for both during the COVID-19 pandemic.MethodsUsing the National Health Interview Survey (NHIS) database, we analyzed data for 15,989 children aged 5–17 and their corresponding parents. The correlations between childhood hearing impairment and mental outcomes were examined using logistic regression models. The 2020 (quarter 3 and quarter 4)-2021 NHIS data was singled out and re-analyzed, focusing on the utilization of medical care during the COVID-19 pandemic.ResultsAfter accounting for covariates, hearing-impaired children exhibited a higher frequency of anxiety (OR 2.33, 95% CI 1.79–3.02) or depression (OR 2.14, 95% CI 1.59–2.88). Parents of hearing-impaired children had significantly higher odds of a higher frequency of anxiety (OR 1.55, 95% CI 1.20–2.01) or depression (OR 1.73, 95% CI 1.30–2.29). Interaction effect of hearing impairment with survey year on parents’ mental health outcomes was observed (p for interaction &lt;0.1). Children with hearing loss had higher odds of reporting delayed medical care (OR 2.00, 95% CI 1.11–3.59) or canceled medical care (OR 1.96, 95% CI 0.98–3.96, p = 0.059) due to the pandemic. Delayed medical care (OR 12.41, 95% CI 2.78–55.46) or canceled medical care (OR 6.26, 95% CI 1.28–30.75) due to the COVID-19 pandemic significantly contributed to the increase of anxiety frequency in hearing-impaired children.ConclusionChildhood hearing impairment exhibits a substantial impact on children’s and parental mental health, which is further exacerbated by the COVID-19 pandemic. Families of hearing-impaired children appear to be in a vulnerable position during public health emergencies such as the COVID-19 pandemic, which can further exacerbate their mental outcomes.
Proteomic analysis of the SMN complex reveals conserved and etiologic connections to the proteostasis network
Authors: Matera A. Gregory , Steiner Rebecca E. , Mills C. Allie , McMichael Benjamin D. , Herring Laura E. , Garcia Eric L. 
Published: September 2024

IntroductionMolecular chaperones and co-chaperones are highly conserved cellular components that perform a variety of duties related to the proper three-dimensional folding of the proteome. The web of factors that carries out this essential task is called the proteostasis network (PN). Ribonucleoproteins (RNPs) represent an underexplored area in terms of the connections they make with the PN. The Survival Motor Neuron (SMN) complex is an assembly chaperone and serves as a paradigm for studying how specific RNAs are identified and paired with their client substrate proteins to form RNPs. SMN is the eponymous component of a large complex, required for the biogenesis of uridine-rich small nuclear ribonucleoproteins (U-snRNPs), that localizes to distinct membraneless organelles in both the nucleus and cytoplasm of animal cells. SMN protein forms the oligomeric core of this complex, and missense mutations in the human SMN1 gene are known to cause Spinal Muscular Atrophy (SMA). The basic framework for understanding how snRNAs are assembled into U-snRNPs is known. However, the pathways and mechanisms used by cells to regulate their biogenesis are poorly understood.MethodsGiven the importance of these processes to normal development as well as neurodegenerative disease, we set out to identify and characterize novel SMN binding partners. We carried out affinity purification mass spectrometry (AP-MS) of Drosophila SMN complexes using fly lines exclusively expressing either wildtype or SMA-causing missense alleles.ResultsBioinformatic analyses of the pulldown data, along with comparisons to proximity labeling studies carried out in human cells, revealed conserved connections to at least two other major chaperone systems including heat shock folding chaperones (HSPs) and histone/nucleosome assembly chaperones. Notably, we found that heat shock cognate protein Hsc70-4 and other HspA family members preferentially associated with SMA-causing alleles of SMN.DiscussionHsc70-4 is particularly interesting because its mRNA is aberrantly sequestered by a mutant form of TDP-43 in mouse and Drosophila ALS (Amyotrophic Lateral Sclerosis) disease models. Most important, a missense allele of Hsc70-4 (HspA8 in mammals) was recently identified as a bypass suppressor of the SMA phenotype in mice. Collectively, these findings suggest that chaperone-related dysfunction lies at the etiological root of both ALS and SMA.
Breaking barriers: how the rural poor engage in tourism activities without external support in selected Iranian villages
Authors: Torabi Zabih-Allah 
Published: September 2024

BackgroundThis study examines the participation of the rural poor in tourism activities without external support in selected Iranian villages, addressing the research gap on their direct involvement in formal and informal tourism businesses.MethodologyA qualitative approach was employed, utilizing face-to-face semi-structured interviews with 65 participants from three villages near Turan National Park in Shahrud, Iran. Data were analyzed using thematic analysis.FindingsResults indicate that the rural poor actively engage in tourism. This participation encompasses seeking formal employment in tourism facilities, engaging in informal activities, and acquiring relevant knowledge and skills. The study highlights the significant potential of informal tourism businesses in increasing opportunities for low-income individuals, despite challenges such as lack of capital and legal protection.ConclusionThe findings demonstrate that even without external assistance, the rural poor find ways to participate in and benefit from tourism activities. Both formal and informal tourism activities play important roles in poverty alleviation efforts. The study emphasizes the need for targeted interventions to support the rural poor's participation in the tourism sector and harness tourism's potential for poverty reduction in rural areas.
Pre-, Per- and post-cooling strategies used by competitive tennis players in hot dry and hot humid conditions
Authors: Robin Nicolas , Crespo Miguel , Ishihara Toru , Carien Robbin , Brechbuhl Cyril , Hue Olivier , Dominique Laurent 
Published: September 2024

PurposeThis research investigated the pre-, per- and post cooling strategies used by competitive tennis players from various levels of play who occasionally train and compete in hot (&gt;28°C) and humid (&gt;60% rH), and dry (&lt;60% rH) environments.Methods129 male tennis players (Mage = 24.9) competing at regional (N = 54), national (N = 30) and international (N = 45) levels, completed an online questionnaire regarding their use (i.e., timing, type, justification and effectiveness) of pre- (i.e., before practice), per- (i.e., during exercise) and post-cooling strategies when playing tennis in hot dry (HD) and hot humid (HH) conditions. Individual follow-up interviews were also carried on 3 participants to gain an in-depth understanding of the player's experience.ResultsCompetitive tennis players used both internal and external cooling strategies to combat the negative effects of HD and HH conditions, but considered the HH to be more stressful than HD and experienced more heat-related illness in HH environments. International players used cold packs and cold towel more frequently than the regional and national players in hot environments, and used cold water immersion and cold vest more frequently than the latter in HH. Differences in strategy use were mostly observed during per-cooling where regional and national players more frequently used cold drinks than international players who more frequently used cold packs in HD and cold towel in HH conditions. Moreover the latter more frequently used cold towel, cold packs and cold water immersion as post-cooling strategies than regional players.ConclusionWhen playing tennis in the heat, it is strongly recommended to employ cooling strategies to maintain health, limit declines in performance, and promote recovery. We also recommend improving education regarding the appropriate use and effectiveness of cooling strategies, and increasing their availability in tournaments.
Consensus SE(3)-Constrained Extended Kalman Filter for Close Proximity Orbital Relative Pose Estimation
Authors: Mathavaraj S. , Butcher Eric A. 
Published: September 2024

In this paper, a recently proposed SE(3)-constrained extended Kalman filter (EKF) is extended to formulate a strategy for relative orbit estimation in a space-based sensor network. The resulting consensus SE(3)-constrained EKF utilizes space-based sensor fusion and is applied to the problem of spacecraft proximity operations and formation flying. The proposed filter allows for the state (i.e., pose and velocities) estimation of the deputy satellite while accounting for measurement error statistics using the rotation matrix to represent attitude. Via a comparison with a conventional filter in the literature, it is shown that the use of the proposed consensus SE(3)-constrained EKF can improve the convergence performance of the existing filter for satellite formation flying. Moreover, the benefits of faster convergence and consensus speed by using communication networks with more connections are illustrated to show the significance of the proposed sensor fusion strategy in spacecraft proximity operations.
Dynamic Transcriptome Profile Analysis of Mechanisms Related to Melanin Deposition in Chicken Muscle Development
Authors: Ji Gaige , Zhang Ming , Ju Xiaojun , Liu Yifan , Shan Yanju , Tu Yunjie , Zou Jianmin , Shu Jingting , Li Hua , Zhao Weidong 
Published: September 2024

The pectoral muscle is an important component of skeletal muscle. The blackness of pectoral muscles can directly affect the economic value of black-boned chickens. Although the genes associated with melanogenesis in mammals and birds have been thoroughly investigated, only little is known about the key genes involved in muscle hyperpigmentation during embryonic development. Here, we analyzed melanin deposition patterns in the pectoral muscle of Yugan black-boned chickens and compared differentially expressed genes (DEGs) between the muscles of Wenchang (non-black-boned chickens) and Yugan black-boned chickens on embryonic days 9, 13, 17, and 21. Melanin pigments were found to gradually accumulate in the muscle fibers over time. Using RNA-seq, there were 40, 97, 169, and 94 genes were identified as DEGs, respectively, between Yugan black-boned chicken muscles and Wenchang chickens at embryonic day 9, 13, 17, and 21 stages (fold change ≥2.0, false discovery rate (FDR) < 0.05). Thirteen DEGs, such as MSTRG.720, EDNRB2, TYRP1, and DCT, were commonly identified among the time points observed. These DEGs were mainly involved in pigmentation, melanin biosynthetic and metabolic processes, and secondary metabolite biosynthetic processes. Pathway analysis of the DEGs revealed that they were mainly associated with melanogenesis and tyrosine metabolism. Moreover, weighted gene co-expression network analysis (WGCNA) was used to detect core modules and central genes related to melanogenesis in the muscles of black-boned chickens. A total of 24 modules were identified. Correlation analysis indicated that one of them (the orange module) was positively correlated with muscle pigmentation traits (r > 0.8 and p < 0.001). Correlations between gene expression and L* values of the breast muscle were investigated in Yugan and Taihe black-boned chickens after hatching. The results confirmed that EDNRB2, GPNMB, TRPM1, TYR, and DCT expression levels were significantly associated with L* values (p < 0.01) in black-boned chickens (p < 0.05). Our results suggest that EDNRB2, GPNMB, TRPM1, TYR, and DCT are the essential genes regulating melanin deposition in the breast muscle of black-boned chickens. MSTRG.720 is a potential candidate gene involved in melanin deposition in the breast muscles of Yugan black-boned chickens.
GPs’ experience of difficult decisions in people with dementia who have an acute illness: a qualitative, semi-structured interview study
Authors: Lassa Samuel , Burton Chris , Dickson Jon M 
Published: September 2024

BackgroundGPs are often required to make decisions about the management of acute illness in people living with dementia. These decisions are often complex and involve multiple informants.AimWe aimed to explore how GPs made decisions about acute illness in people with dementia using a micropolitics approach.Design & settingQualitative, semi-structured interviews with 13 GPs with a range of years of experience working in South Yorkshire, UK.MethodInterviews were conducted by phone. Interviews focused on GPs’ accounts to reflect their own perceptions and choices as portrayed to the interviewer. The analysis used the lens of micropolitics in the analysis and interpretation of the themes, with a focus on decisions between GP, patient, family and carers, and other health and social care providers about the management of acute illness in a person with dementia.ResultsThe results showed that GPs act as street-level bureaucrats while carrying out their role, using discretion during decision-making in an environment characterised by uncertainties and work pressures. In addition, GPs use the ‘soft power’ skills of diplomacy, such as negotiation, persuasion, and engagement, in navigating difficult decision-making situations, while building relationships and partnerships with various actors in the health system.ConclusionGPs possess and express power, and influence decision-making in people living with dementia when navigating biomedical, social, and psychological factors. This power comes in the form of soft power (street-level diplomacy) and the more formal power of street-level bureaucracy.
Changes in 24-Hour Urine Chemistry in Patients with Nephrolithiasis during Weight Loss with Glucagon-Like Peptide 1–Based Therapies
Authors: Feghali Karen , Li Xilong , Maalouf Naim M. 
Published: September 2024

Key Points

In obese kidney stone formers, weight loss with glucagon-like peptide-1 (GLP)-based therapy was associated with a significant decline in 24-hour urine oxalate and sulfate excretion rates.Weight loss through GLP-based therapies was associated with nonsignificant changes in urine saturation indices.In obese kidney stone formers, GLP-based therapy appears to be a safe option for weight loss on the basis of 24-hour urine studies.

Background
Obesity is an independent risk factor of incident and recurrent nephrolithiasis. The effect of weight loss through glucagon-like peptide 1 (GLP-1) receptor agonists and dual GLP-1/gastric inhibitory polypeptide receptor agonists (GLP-based therapies) on nephrolithiasis is not well understood. This study examined the changes in 24-hour urine chemistry assessing for stone risk during weight loss through GLP-based therapies.

Methods
This retrospective analysis identified adult stone formers followed at our academic institution's weight wellness clinic between September 2015 and August 2023 and included patients with at least two 24-hour urine collections for stone risk assessment. 24-hour urine parameters before and during weight loss in patients on GLP-based therapies were compared.

Results
Forty-four obese patients with nephrolithiasis experienced significant weight reduction (−6.6±7.3 kg, P < 0.001) over a median 1.1 years of follow-up with GLP-based therapies. During this period, there was a significant decrease in 24-hour urine oxalate (40±16 to 32±11 mg/d, P = 0.002), sulfate (21±10 to 17±9 mmol/d, P = 0005), and ammonium (35±22 to 29±15 mEq/d, P = 0.01) excretion rates. There were nonsignificant changes in urine calcium, citrate, uric acid, pH, phosphorus, sodium, potassium, magnesium, chloride, creatinine, or total volume. In addition, there was no statistical difference in urine supersaturation indices with respect to calcium oxalate, calcium phosphate, and uric acid.

Conclusions
Our results indicate that weight loss through GLP-based therapies is not associated with prolithogenic changes in 24-hour urine chemistry in patients with nephrolithiasis, unlike what happens with other weight loss modalities.