bluuebunny/crossref_metadata_2025_split huggingface dataset:
Swapping of transmembrane domains in the epithelial calcium channel TRPV6
Authors: Singh Appu K. , Saotome Kei , Sobolevsky Alexander I. 
Published: May 2017

AbstractTetrameric ion channels have either swapped or non-swapped arrangements of the S1-S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangementin vivo, affecting its function in normal or disease states.
Mutations in disordered regions cause disease by creating endocytosis motifs
Authors: Meyer Katrina , Uyar Bora , Kirchner Marieluise , Cheng Jingyuan , Akalin Altuna , Selbach Matthias 
Published: May 2017

AbstractMutations in intrinsically disordered regions (IDRs) of proteins can cause a wide spectrum of diseases. Since IDRs lack a fixed three-dimensional structure, the mechanism by which such mutations cause disease is often unknown. Here, we employ a proteomic screen to investigate the impact of mutations in IDRs on protein-protein interactions. We find that mutations in disordered cytosolic regions of three transmembrane proteins (GLUT1, ITPR1 and CACNA1H) lead to an increased binding of clathrins. In all three cases, the mutation creates a dileucine motif known to mediate clathrin-dependent trafficking. Follow-up experiments on GLUT1 (SLC2A1), a glucose transporter involved in GLUT1 deficiency syndrome, revealed that the mutated protein mislocalizes to intracellular compartments. A systematic analysis of other known disease-causing variants revealed a significant and specific overrepresentation of gained dileucine motifs in cytosolic tails of transmembrane proteins. Dileucine motif gains thus appear to be a recurrent cause of disease.
Alpha oscillations during incidental encoding predict subsequent memory for new “foil” information
Authors: Vogelsang David A. , Gruber Matthias , Bergström Zara M. , Ranganath Charan , Simons Jon S. 
Published: May 2017

AbstractPeople can employ adaptive strategies to increase the likelihood that previously encoded information will be successfully retrieved. One such strategy is to constrain retrieval towards relevant information by re-implementing the neurocognitive processes that were engaged during encoding. Using electroencephalography (EEG), we examined the temporal dynamics with which constraining retrieval towards semantic versus non-semantic information affects the processing of new “foil” information encountered during a memory test. Time-frequency analysis of EEG data acquired during an initial study phase revealed that semantic compared to non-semantic processing was associated with alpha decreases in a left frontal electrode cluster from around 600ms after stimulus onset. Successful encoding of semantic versus non-semantic foils during a subsequent memory test was related to decreases in alpha oscillatory activity in the same left frontal electrode cluster, which emerged relatively late in the trial at around 1000–1600ms after stimulus onset. Across subjects, left frontal alpha power elicited by semantic processing during the study phase correlated significantly with left frontal alpha power associated with semantic foil encoding during the memory test. Furthermore, larger left frontal alpha power decreases elicited by semantic foil encoding during the memory test predicted better subsequent semantic foil recognition in an additional surprise foil memory test. These findings indicate that constraining retrieval towards semantic information involves re-implementing semantic encoding operations that are mediated by alpha oscillations, and that such re-implementation occurs at a late stage of memory retrieval perhaps reflecting additional monitoring processes.
Rob and MarA alter susceptibility ofEscherichia colito antibiotics in presence of salicylate
Authors: Jain Kirti , Saini Supreet 
Published: May 2017

AbstractWhen exposed to stress, bacterial cells launch a diverse response to enhance their chances of survival. This response involves modulation of expression of a large number of proteins which help the cell counter stress. This modulation is facilitated by several transcription factors in bacteria and inE. colithree homologous regulators, MarA, Sox, and Rob are known to launch a coordinated response to combat various stress environments. MarA and SoxS are known to control multiple antibiotic resistance and superoxide regulon respectively. Rob has been observed to control similar downstream targets as MarA and SoxS. However, physiological relevance of Rob in not understood. We show that Rob along with MarA, in presence of inducer salicylate, can help cell survive in presence of lethal concentration of wide range of antibiotics.
Genome-wide sequence and expression analysis of the NAC transcription factor family in polyploid wheat
Authors: Borrill Philippa , Harrington Sophie A. , Uauy Cristobal 
Published: May 2017

ARTICLE SUMMARYTranscription factors are vital in plants to regulate gene expression in response to environmental stimuli and to control developmental processes. In this study, we annotated and classified transcription factors in polyploid bread wheat into gene families and explored the NAC family in detail. We combined phylogenetic analysis and transcriptome analysis, using publicly available RNA-seq data, to characterize the NAC gene family and provide hypotheses for putative functions of many NAC transcription factors. This study lays the groundwork for future studies on transcription factors in wheat which may be of great agronomic relevance.ABSTRACTMany important genes in agriculture correspond to transcription factors which regulate a wide range of pathways from flowering to responses to disease and abiotic stresses. In this study, we identified 5,776 transcription factors in hexaploid wheat (Triticum aestivum) and classified them into gene families. We further investigated the NAC family exploring the phylogeny, C-terminal domain conservation and expression profiles across 308 RNA-seq samples. Phylogenetic trees of NAC domains indicated that wheat NACs divided into eight groups similar to rice (Oryza sativa) and barley (Hordeum vulgare). C-terminal domain motifs were frequently conserved between wheat, rice and barley within phylogenetic groups, however this conservation was not maintained across phylogenetic groups. We explored gene expression patterns across a wide range of developmental stages, tissues, and abiotic stresses. We found that more phylogenetically related NACs shared more similar expression patterns compared to more distant NACs. However, within each phylogenetic group there were clades with diverse expression profiles. We carried out a co-expression analysis on all wheat genes and identified 37 modules of co-expressed genes of which 23 contained NACs. Using GO term enrichment we obtained putative functions for NACs within co-expressed modules including responses to heat and abiotic stress and responses to water: these NACs may represent targets for breeding or biotechnological applications. This study provides a framework and data for hypothesis generation for future studies on NAC transcription factors in wheat.
Filament Rigidity and Connectivity Tune the Deformation Modes of Active Biopolymer Networks
Authors: Stam Samantha , Freedman Simon L. , Banerjee Shiladitya , Weirich Kimberly L. , Dinner Aaron R. , Gardel Margaret L. 
Published: May 2017

ABSTRACTMolecular motors embedded within collections of actin and microtubule filaments underlie the dynamic behaviors of cytoskeletal assemblies. Understanding the physics of such motor-filament materials is critical to developing a physical model of the cytoskeleton and the design of biomimetic active materials. Here, we demonstrate through experiments and simulations that the rigidity and connectivity of filaments in active biopolymer networks regulates the anisotropy and the length scale of the underlying deformations, yielding materials with varying contractility. Semi-flexible filaments that can be compressed and bent by motor stresses undergo deformations that are predominantly biaxial. By contrast, rigid filament bundles contract via actomyosin sliding deformations that are predominantly uniaxial. Networks dominated by filament buckling are robustly contractile under a wide range of connectivities, while networks dominated by actomyosin sliding can be tuned from contractile to extensile through reduced connectivity via cross-linking. These results identify physical parameters that control the forces generated within motor-filament arrays, and provide insight into the self-organization and mechanics of cytoskeletal assemblies.
Policy support for own-brand innovation in China’s auto industry: panacea or placebo?
Authors: Yang Dongjin , Tachia Chin , Ren-huai Liu , Yao Zuowei 
Published: May 2017

Purpose
China has become the world’s largest vehicle market, because of the strong governmental support to boost car sales and particularly because of the establishment of joint ventures between state-owned enterprises and world-class automakers. However, because many Sino-foreign joint ventures have performed unsatisfactorily in terms of creating indigenous brands, the purpose of this paper is to explore the cause-and-effect relationships among governmental policy support, Sino-foreign joint ventures and own-brand innovation in China’s passenger-car industry.

Design/methodology/approach
After briefly introducing the development history of the Chinese auto industry and reviewing relevant literature, first, the analytic hierarchy process method is used to create a unique, context-specific equation to measure the degree of policy support in China. This paper then uses the hierarchical multiple regression method to process the 2014 public data set.

Findings
The findings show that the degree of policy support increases the preference of the firms for producing foreign-brand cars, while such a relationship is fully mediated by the establishment of Sino-foreign joint ventures.

Research limitations/implications
The research brings greater and deeper insights into the interplay among governmental policy, the conduct of own-brand strategy and international joint ventures in China’s auto market, showing that policy support may not always be beneficial, but sometimes be detrimental to indigenous innovation.

Originality/value
This paper can be seen as an exciting step that adds to a better understanding of the role of political support in shaping the strategic choices of firms in terms of brand innovation in the Chinese automobile industry. The proposed novel, context-specific approach for evaluating the degree of policy support embodies the distinctive institutional complexity and intricate social network embedded in the local car market during the period of China’s socio-economic transformation – an approach that is original in this field.
The performance effect of micro-innovation in SMEs: evidence from China
Authors: Zhou Qing , Fang Gang , Yang Wei , Wu Yun , Ren Liqin 
Published: May 2017

PurposeThe purpose of this paper is to theoretically and empirically analyze the impact of the types of micro-innovation on innovation performance and the choice of micro-innovation strategies in different contexts on the basis of an examination of the basis and standards of micro-innovation categorization.Design/methodology/approachThe authors collected and analyzed 206 survey samples from the Zhejiang Province in China; there were 68, 63 and 75 enterprises at inception, high growth and maturity stages, respectively, and there were, in total, 53, 90 and 63 low-tech manufacturing small and medium enterprises (SMEs), technology-intensive manufacturing SMEs and service-oriented SMEs, respectively. Multiple regression analysis was used to test the hypotheses.FindingsThe analysis results suggest that SMEs at the embryonic stage should choose strategies of imitative innovation, whereas SMEs at growth stage should use continued micro-innovation and independent micro-innovation as the optimal choices; it is better for the SMEs at the mature stage to resort to independent micro-innovation. Low-tech manufacturing SMEs should opt for the continued micro-innovation strategy, and technology-intensive manufacturing SMEs should adopt independent micro-innovation, whereas service-oriented SMEs should choose both continued and independent micro-innovation strategies.Originality/valueThis study sets up a classification framework of micro-innovation and addresses its category and sources, thus extending the micro-innovation research results. The conclusion also supports and enriches the view of open innovation in the innovation theory. Hidden behind the phenomenon that internal and external factors play vital roles, it is a basic rule that innovation activities must be subject to various related factors.
Bridging the gap: reflections on theorizing with managers
Authors: Leeflang Peter S.H. 
Published: May 2017

Purpose
The purpose of this paper is to reflect on Nenonen et al.’s paper on theorizing with managers. This paper discusses differences between marketing science and marketing practice and gives suggestions to reduce the theory–praxis gap. It gives examples of quantitative studies that have been performed with practioners and use the choices proposed by Nenonen et al. to draw conclusions.

Design/methodology/approach
This paper is based on personal experience of the author, which is supported by a substantial number of empirical studies.

Findings
Cooperation with marketing managers/decision-makers is most successful when the initiator is the practitioner. Public policy and litigation are the most promising areas for cooperation. It is difficult to publish the outcomes of these “joint” studies in top journals.

Originality/value
This study extends and continues the results of the study by Nenonen et al.
The impact of regulatory focus and word of mouth valence on search and experience attribute evaluation
Authors: Roy Rajat , Naidoo Vik 
Published: May 2017

Purpose
This paper aims to investigate the direct and interactive effects of regulatory focus (promotion versus prevention), attribute type (search versus experience) and word of mouth valence (positive versus negative) on consumption decision for a service and a product.

Design/methodology/approach
Three empirical studies (two laboratories and a field experiment) using “university” and “mobile phone” as the research setting were used to test the key hypotheses.

Findings
Promotion (prevention)-focused subjects preferred experience (search) attributes over their counterparts while making consumption decision. This preference was further reinforced for both promotion and prevention-focused people under positive word of mouth. Under negative word of mouth, in comparison to their counterparts, promotion-focused people still retained their preference for experience attributes, whereas prevention-focused subjects reversed their preference and maintained status quo.

Research limitations/implications
Future research may validate and extend authors’ findings by looking into the underlying process or studying additional word of mouth variables that may moderate the current findings.

Practical implications
The findings will help managers devise a range of marketing strategies in the areas of advertising and product positioning, especially for products/services that are showcased in terms of experience and search attributes.

Originality/value
The current research is novel as no prior research has proposed and tested the two-way interaction between regulatory focus and search/experience attributes, or its further moderation by word of mouth valence.
Being the first entrant and getting stuck in the middle
Authors: Montaguti Elisa , Zammit Alessandra 
Published: May 2017

Purpose
This paper aims to examine how pioneering advantage interacts with the compromise effect generated by new product entries. Building on prior work on pioneering advantage and extreme aversion, this research moves toward understanding how the choice share of a pioneer realigns as a consequence of new product entries generating compromise-like scenarios.

Design/methodology/approach
The authors run three experiments to test their propositions. The authors present one study which documents the effect. The second study provides process evidence. The third study suggests how brands can neutralize the adverse effect on their share generated by the followers’ entry/positioning.

Findings
In three studies, the authors showed that when a pioneering product becomes intermediate in a choice set, its share is more adversely affected than when it becomes extreme. The authors show that this depends on consumers’ propensity to use non-compensatory decision rules in the presence of a pioneering alternative. The authors also document that the relative disadvantage of the intermediate pioneer can be overcome when the reasons for selecting an intermediate alternative based on a compensatory decision rule are restored.

Practical implications
The research provides guidelines for managers wanting to enter product categories where a pioneer already exists. The authors show that opting for an extreme position that renders the pioneer intermediate can be rewarding. In contrast, being the second extreme player in a market where the pioneer becomes extreme reduces the expected share of this last entrant.

Originality/value
The authors’ contribution is in showing that this decision strategy can clash with the rule consumers generally use in a compromise setting and that this clash generates two different effects when the pioneer becomes intermediate or extreme.
The effect of specialization on operational performance
Authors: Halkjær Søren , Lueg Rainer 
Published: May 2017

PurposeThe purpose of this paper is to analyze how specialization in hospitals affects operational performance, measured by the length of stay and readmission rate. The authors assess a public policy change in the Danish healthcare sector from 2011 which required that some hospital services had to be centralized leading to specialization within the merged departments.Design/methodology/approachTaking an institutional theory perspective, the authors conduct a natural experiment. The data include 24,694 observations of urological patient treatments from 2010 to 2012.FindingsThe econometric difference-in-difference analysis finds that the readmission rate decreases by approximately four percentage points in the departments affected by the policy change. Contrary to expectations, the length of stay increases by 0.38 days. The authors complement the natural experiment with a mixed-methods approach that includes proprietary data from the management control system of the hospital, public documentation on the policy change, as well as interviews with key informants. These data suggest that operational deficiency is related to the fact that specialization was externally enforced through the public policy change. The authors illustrate how the hospital staff struggle for legitimacy after this policy change, and how cost savings obstructed the specialized department in achieving its goals.Originality/valueThe authors conclude that the usual economies-of-scales-based logic of (higher)volume-(better)outcome studies cannot easily be transferred to specialization in hospitals, unless one accounts for the institutional reason of the specialization.
Do corporate boards affect firm performance? New evidence from an emerging economy
Authors: Yasser Qaiser Rafique , Mamun Abdullah Al , Seamer Michael 
Published: May 2017

PurposeThe purpose of this paper is to examine an association between board demographics and corporate performance using a sample of Pakistani firms listed on the Pakistan Stock Exchange in the 2014 year.Design/methodology/approachThis study is unique in that corporate performance is examined using a mixture of performance measures: accounting-based measures (return on assets), market-based measures (Tobin’sQ, earnings per share, and total return) and economic profit measures (economic value added).FindingsThe results of this research show a significant positive relationship between board size, minority representation on the board and the appointment of a family director and enhanced firm performance. However, contrary to expectations, the authors also find that instead of adding value, the appointment of independent directors to Pakistani firm boards negatively impacts firm value.Originality/valueThis study adds to a growing body of empirical evidence that suggests that agency theory-based corporate governance recommendations adopted in developed economies may not be relevant to emerging economy firms.
Principles of performance dialogue in public administration
Authors: Laihonen Harri , Mäntylä Sari 
Published: May 2017

Purpose
The literature acknowledges the importance of interpretative processes, discussion, and organizational learning in public performance management, but a knowledge gap remains concerning the mechanism of performance dialogue. To fill this gap, the purpose of this paper is to study the principles of performance dialogue and collaborative performance management in public administration.

Design/methodology/approach
The study utilizes a longitudinal research setting and analyzes the evolution of performance management practices in one city organization in Finland.

Findings
The study suggests that performance dialogue needs to be integrated with management practices and explains how this can be done. Three guiding principles of performance dialogue and collaborative performance management are derived. These underline the role of “referees of the information game”, a supportive and encouraging environment and a focus on the use of performance information.

Practical implications
Performance information is too often provided as a back-office function, and dialogue with information users is either completely lacking or somehow disturbed. The performance dialogue provides a platform for collaborative sense making and helps managers to better understand the complex phenomena and processes they are responsible for.

Originality/value
The literature dealing specifically with the change from centralized and vertical performance management practices toward decentralized and horizontal practices is still scarce. This paper provides a new perspective on management control and organizational learning in public administration based on performance dialogue.