bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving Growth and Nutrient Quality of Mustard Varieties Through Rhizobacterial Inoculation
Authors: Basharat Noman , Rashid Madiha , Noreen Zahra , Aslam Muhammad , Usman Sheeraz , Shah Anis Ali , Shakeel Muhammad , Shaffique Shifa , Gatasheh Mansour K. 
Published: October 2024

Abstract

The excessive feeding of chemical fertilizers into the soil has affected its fertility. The continued use of these fertilizers can impose serious environmental hazards on mankind. Biofertilizers are an alternative strategy and could improve the growth of different crops. The study was conducted in a completely randomized design to understand the effect of soil-borne rhizospheric bacteria (Bradyrhizobium japonicum & Burkholderia cepacia) on morphology, physiology, yield, antioxidants activity and protein content of four Mustard (Brassica juncea (L.) Czern.) varieties. Both the rhizospheric bacteria were cultured in LB (Luria-Bertani) broth to prepare the bacterial suspension. A single pot was inoculated with 25 ml of the bacterial suspension. The combined and individual treatments of both bacteria resulted in an increase in the plant heights, shoot and root lengths, shoot and root fresh weights, shoot and root dry weights and leaf yield, compared to control. The number of pods, weight of 1000 seeds, pod length and number of seeds per pod was also enhanced with these bacterial treatments. The B. japonicum treatment uplifted the total chlorophyll, carotenoids, and relative water content in mustard varieties. The treatment of B. cepacia raised the phenolic content, total soluble protein, and the CAT, and POD activity in Mustard plants. The results revealed that the use of B. japonicum and B. cepacia could be a better choice for plant breeders as a biofertilizer. However, the effects could vary depending upon the type of crops, as well as the varieties of the same crop.
Recycled cathode from Li-ion batteries applied to adsorption and demethylation of methylene blue for thionine formation
Authors: Garcia Eric M. , Teixeira Rodrigo G. , Taroco Hosane A. , Melo Julio O.F , Taroco Cristiane G. 
Published: October 2024

Abstract

This study explores the demethylation of methylene blue in an acidic pH, utilizing spent cathode from Li-ion batteries. The spent cathode has a composition of LiMn2O4 and a pcz close to pH = 2.1. In contact with methylene blue in an acidic medium (pH = 2), the spent cathode adsorbed and demethylated methylene blue to form thionine. This was confirmed by UV-vis and ESI measurements of the resulting solutions. The intermediate m/z = 300 was crucial for proposing the demethylation mechanism that is similar to a demethylation mechanism catalyzed by hemoproteins. FTIR measurements confirmed the adsorption of methylene blue onto the spent cathode. The rise in pH observed during demethylation can explain to the incomplete degradation of methylene blue stopping at the thionine stage. Consequently, the reaction involving spent cathode of Li-ion battery based on LiMn2O4 may be applicable to the demethylation of other molecules in future research.
Integrated modeling of surface water and groundwater interactions in the Eastern Mitidja Plain, North Algeria: investigating the impact of human activities and climate change
Authors: Kastali Fatima , Meddi Mohamed , Boufekane Abdelmadjid , Longobardi Antonia 
Published: October 2024

ABSTRACT

The interaction between surface water and groundwater is a crucial factor in understanding water resources' dynamics and in promoting sustainable groundwater development, particularly in regions affected by human activities and climate change. This work aims to investigate dynamic interactions between El Hamiz river and Eastern Mitidja alluvial aquifer (North Algeria) using a regional three-dimensional groundwater numerical model (MODFLOW 6). A typical scenario analysis approach was conducted using historical and projected climate data from CMIP5 and CMIP6 models, as well as current and projected groundwater abstraction rates. The findings demonstrate major shifts in the flow dynamics over time. In 1982, the aquifer replenished the river at a rate of 2.59 m3/s. However, in 2019 a new state was observed in which the aquifer was recharged by the river at a rate of 0.73 m3/s. This decrease in flow rate can be linked to over-pumping and decreased recharge rates, which are expected to continue in the future. Future forecasts show that groundwater misuse affects groundwater dynamics more than climate change. However, the Algerian government's projected saltwater desalination and upgraded irrigation methods could provide a substantial contribution to the sustainable growth of groundwater resources, with a potential increase of up to 14 meters.
Estimation of missing streamflow data using various artificial intelligence methods in peninsular Malaysia
Authors: Ng Jing Lin , Huang Yuk Feng , Chong Aik Hang , Ahmed Ali Najah , Syamsunurc Deprizon 
Published: October 2024

ABSTRACT
Missing streamflow data is a common issue in Peninsular Malaysia, as the technologies used in hydrological studies often fail to collect data accurately. Additionally, conventional methods are still widely used in the region, which are less accurate compared to artificial intelligence (AI) methods in estimating missing streamflow data. Therefore, this study aims to estimate the missing streamflow data from 11 stations in Peninsular Malaysia by using different AI methods and determine the most appropriate method. Four homogeneity tests were applied to check the quality of data, and the results of the tests indicated that the streamflow data in most stations were homogenous. Two AI methods were applied in this study, which were artificial neural network and artificial neuro-fuzzy inference systems (ANFIS). The proposed AI methods were compared with five different conventional methods. All streamflow missing data, constituting 30% of data from each year were estimated on a daily time scale, and evaluated using root mean square error, mean absolute error and correlation coefficient values. The results indicated that ANFIS was the best due to its learning abilities and the fuzzy inference systems, which enable it to handle complicated input–output patterns and provide highly accurate estimation results.
Practical implications of adding powdered activated carbon in advanced wastewater treatment for process and plant design
Authors: Slavik Irene , Bechle Thomas S. , Uhl Wolfgang 
Published: October 2024

ABSTRACT

The addition of powdered activated carbon (PAC) in advanced wastewater treatment is increasingly recognized for its effectiveness in removing micropollutants from secondary effluents. This study investigates the implications of PAC dosing on treatment performance, particularly in terms of natural and effluent organic matter (OM) removal, turbidity reduction, and sludge characteristics. Results indicate that while PAC incorporation significantly enhances the adsorption of OM, it necessitates higher coagulant dosages to achieve comparable or improved turbidity levels. The study also reveals that lower pH levels facilitate the removal of OM, thereby increasing the availability of adsorption sites on PAC for micropollutants. Moreover, PAC addition results in the formation of larger, denser flocs that settle faster, leading to a reduction in sludge volume by approximately 20%. However, the increased coagulant demand and the subsequent rise in sludge volume highlight the need for the optimized process design to balance treatment efficiency with operational costs. This research provides valuable insights for the design and operation of wastewater treatment plants, emphasizing the importance of tailored coagulant dosing and process adjustments when integrating PAC into existing treatment frameworks.
Advances in the Study of the Pathogenesis of Vogt-Koyanagi-Harada Syndrome
Authors: Wang Meng-nan , Zhang Lin , Zhou Hong-Yan , Zhong Wei , Zhang Hong , Bi Miao-Miao , Wang Li , Lu Jia 
Published: October 2024

Abstract:
Vogt-Koyanagi-Harada syndrome (VKHS) is a common type of uveitis
characterized by the invasion of melanocyte-rich tissues. In recent years, the incidence
of VKHS has been increasing yearly, and its specific pathogenesis has not yet been
elucidated. However, its pathogenesis has been a hot topic of research. The clinical
course of VKHS is characterized by the early involvement of the posterior segment of
the eye, including exudative retinal detachment, optic papillitis, bilateral diffuse
chorioretinitis, etc. If treated improperly or with delayed treatment, the inflammation
may gradually spread to the anterior segment of the eye, leading to vision loss or even
vision. This study examines the pathogenesis of VKHS. It reviews the progress of
research on the pathogenesis of VKHS, which will help to improve the understanding
of VKHS and provide a reference for subsequent studies.
Scientifc Validation and Chromatographic Method Development for the Estimation of Piperine in Herbal Formulation Babbularishta: A Quality Control Strategy for Critical Quality Attributes
Authors: Gidwani Bina , Kalyani Gunjan , Pandey Ravindra Kumar , Shukla Shiv Shankar 
Published: October 2024

Background:
Babbularishta is an Ayurvedic medicine in liquid form used for the
treatment of phthisis, skin diseases, urinary disorders,, and breathing problems. It is also beneficial
in cases of blood disorders, acne, dermatitis, hemoptysis, lung injury, asthma, persistent
cough, whooping cough, etc.

Objective:
The functional role of this study is to develop a simple, novel, precise, accurate, reliable,
and selective RP-HPLC method for the estimation of piperine content in herbal drug formulations
(Babbularista).

Methods:
A traditional approach to method development could fail to meet the desired separation.
Consideration of the current regulatory requirement for analytical method development
and RP-HPLC method for routine analysis of piperine in the herbal formulation has been optimized
using an analytical Quality by Design (QbD) approach. Three laboratory formulations
and three marketed preparations of Babularishta were investigated for organoleptic parameters,
physicochemical parameters, and validation. The mobile phase composition (methanol and water
% as A), pH (B), and flow rate (mL/min as C) were selected as independent variable,. In
contrast, retention time (min. as Y2), area (AU as Y1) is selected as dependent variables.

Results:
The fingerprinting method was developed and optimized by using the AQbD approach.
Piperine content was found to be in Piper nigrum (Marica) 3.01±0.01527 % w/w and in
Piper longum (Pipali) 2.74±0.01154% w/w in Babbularista.

Conclusion:
An attempt has been made to develop quality control parameters of Babbularista
by developing a suitable and sensitive HPLC methods for the quantitation of piperine in herbal
drug formulations (Babbularista). The developed method was found to be simple, accurate, precise,,
and economical for the estimation of piperine in herbal formulations.
Arylcarboxamide Derivatives as Promising HDAC8 Inhibitors: An Overview in Light of Structure-activity Relationship and Binding Mode of
Interaction Analysis
Authors: Banerjee Suvankar , Baidya Sandip Kumar , Jha Tarun , Ghosh Balaram , Adhikari Nilanjan 
Published: October 2024

Abstract:
HDAC8 is associated with several disease conditions as well as various cancers of several
organs and hematological malignancies. To counter such pathophysiological and disease conditions,
inhibition of HDAC8 may be a promising approach for anticancer drug development. In this
article, a detail of arylcarboxamide-based potential HDAC8 inhibitors has been outlined. Considering
their binding pattern of interactions along with the chemical features, effective and selective
novel HDAC8 inhibitors can be designed further. Therefore, modification of these compounds provides
greater possibilities for the development of novel HDAC8 inhibitors. Nevertheless, structural
modification of such arylcarboxamide derivatives may be able to produce potent dual-inhibitory
compounds along with HDAC8 inhibition. Thus, this article is quite useful for exploring and identifying
several possibilities for arylcarboxamide-based HDAC8 inhibitors. Moreover, it can be concluded
that further study of the arylcarboxamide-based HDAC8 inhibitors can be effectively used
for the treatment of different cancerous and non-cancerous diseases.
Effects of ethanol extract from senna leaf (EESL) on inflammation and oxidative stress in mice: A non-targeted metabolomic study
Authors: Huang Xiaoli , Sun Wen , Sun Chang , Tan Jiajun , Wu Liang , Yang Fumeng 
Published: October 2024

Background:
Senna leaf is a commonly used medication for treating constipation, and
long-term use can cause damage to the intestinal mucosa and lead to drug dependence. But the exact
mechanism remains unclear.

Objective:
Using non-targeted metabolomics technology to study the mechanism of senna leaf
ethanol extract (EESL) inducing inflammation and oxidative stress in mice and causing side effects.

Methods:
EESL was administered to mice by gavage to detect inflammation and oxidative stressrelated
factors in mice, and the EESL components and differential metabolites in mouse plasma
were analyzed using non-targeted metabolome techniques.

Results:
23 anthraquinone compounds were identified in the EESL, including sennoside and their
derivatives. Administration of EESL to mice resulted in a significant increase in pro-inflammatory
factors, IL-1β, and IL-6 in the plasma, while the levels of IgA significantly decreased. The levels
of oxidative stress significantly increased, and the intestinal mucosal integrity was impaired. 21 endogenous
in plasma metabolites were identified as differential metabolites related with taurine and
taurine metabolism, glycerophospholipid metabolism, arachidonic acid metabolism, tryptophan
metabolism, and sphingolipid metabolism. These metabolic pathways are related to oxidative
stress and inflammation.

Conclusion:
Senna leaf can inhibit the expression of tight junction proteins in the intestinal mucosa
and disrupt intestinal mucosal barrier integrity, exacerbating oxidative stress and inflammation
induced by bacterial LPS entering the bloodstream. In addition, the impact of Senna leaf on
tryptophan metabolism may be linked to the occurrence of drug dependence.
Preclinical Aspects of [89Zr]Zr-DFO-Rituximab: A High Potential Agent for Immuno-PET Imaging
Authors: Rouhollahi Zahra , Aghamiri Seyed Mahmoud Reza , Yousefnia Hassan , Alirezapour Behrouz , Moghaddasi Ali , Zolghadri Samaneh 
Published: October 2024

Background:
An early diagnosis of cancer can lead to choosing more effective
treatment and increase the number of cancer survivors. In this study, the preparation and preclinical
aspects of [89Zr]Zr-DFO-Rituximab, a high-potential agent for PET imaging of Non-
Hodgkin Lymphoma (NHL), were evaluated.

Methods:
DFO was conjugated to rituximab monoclonal antibody (mAb), and DFO-rituximab
was successfully labeled with zirconium-89 (89Zr) at optimized conditions. The stability of the
complex was assessed in human blood serum and PBS buffer. Radioimmunoreactivity (RIA) of
the radioimmunoconjugate (RIC) was evaluated on CD20-overexpressing Raji cell line and
CHO cells. The biodistribution of the radiolabeled mAb was studied in normal and tumorbearing
rodents. Finally, the absorbed dose in human organs was estimated.

Results:
The radiolabeled compound was prepared with radiochemical purity (RCP)
>99% (RTLC) and a specific activity of 180±1.8 GBq/g. The RCP of the final complex PBS
buffer and human blood serum was higher than 95%, even after 48 h post incubation. The RIA
assay demonstrated that more than 63% of the radiolabeled compound (40 ng/ml, 0.5 mL) was
bound to 5×106 Raji cells. The biodistribution of the final product in tumor-bearing mice
showed a high accumulation of the RIC in the tumor site in all intervals post-injection. Tumor/
non-target ratios were increased over time, and longer imaging time was suggested. The
dosimetry data indicated that the liver received the most absorbed dose after the complex injection.

Conclusion:
[89Zr]Zr-DFO-Rituximab represents a significant advancement in the field of oncological
imaging and offers a robust platform for both diagnostic and therapeutic applications in
the management of B-cell malignancies.
Cadence-Based Pedometer App With Financial Incentives to Enhance Moderate-to-Vigorous Physical Activity: Development and Single-Arm Feasibility Study
Authors: Hayashi Kosuke , Imai Hiromitsu , Oikawa Ichiro , Ishihara Yugo , Wakuda Hirokazu , Miura Iori , Uenohara Shingo , Kuwae Asuka , Kai Megumi , Furuya Ken'ichi , Uemura Naoto 
Published: October 2024

Background
High levels of physical activity are key to improving health outcomes, yet many people fail to take action. Using pedometers to target steps per day and providing financial incentives is a simple and scalable approach to promoting public health. However, conventional pedometers do not account for “intensity” and “duration,” making it challenging to efficiently increase people’s moderate-to-vigorous physical activity (MVPA), which is expected to improve health outcomes. Based on these rationales, we developed a smartphone app that sets step cadence as a goal (defined as a daily challenge of walking more than 1500 steps in 15 minutes twice a day, which is a heuristic threshold for moderate physical activity) and provides financial incentive when the challenge is met.

Objective
This study aimed to evaluate the feasibility of our novel app and explore whether its use can increase users’ daily MVPA.

Methods
A single-arm pre-post study evaluated the feasibility and efficacy of the app. A total of 15 participants used app 1 (an app without financial incentives) for the first period (4 weeks) and then switched to app 2 (an app with financial incentives) for the second period (4 weeks). The primary outcome was the difference between the first and second periods in the number of successful challenge attempts per week. Secondary outcomes were differences between the first and second periods in daily steps and distance walked. Exploratory outcomes included the difference between the first and second periods in daily “heart points” as measured by Google Fit, a publicly available app that measures users’ daily MVPA.

Results
The number of successful challenge attempts per week increased significantly compared to the first period (5.6 times per week vs 0.7 times per week; P<.001). Although not statistically significant, there was a trend toward an increase in the mean steps per day and distance walked per day (6586 steps per day vs 5950 steps per day; P=.19; and 4.69 km per day vs 3.85 km per day; P=.09, respectively). An exploratory end point examining daily MVPA by “heart points” collected from Google Fit also showed a significant increase compared to the first period (22.7 points per day vs 12.8 points per day; P=.02).

Conclusions
Our app using step cadence as a goal and providing financial incentives seemed feasible and could be an effective app to increase users’ daily MVPA. Based on the results of this study, we are motivated to conduct a confirmatory study with a broader and larger number of participants.

Trial Registration
UMIN 000050518; https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000057420
Gambaran Efektivitas Terapi Antiretroviral Paduan Rejimen TLD (Tenofovir + Lamivudin+ Dolutegravir) pada Pasien HIV di RSUP Dr. Mohammad Hoesin Palembang
Authors: Susanti Diah , Nuryastuti Titik , Ikawati Zullies 
Published: October 2024

Dolutegravir (DTG) merupakan obat Antiretroviral (ARV) golongan integrase inhibitor (INSTI) terbaru yang pada tahun 2019 World Health Organization (WHO) merekomendasikannya sebagai pilihan terapi ARV lini pertama dan kedua pada pasien Human Immunodeficiency Virus (HIV). Di Indonesia, DTG direkomendasikan pada Juli 2020 sebagai penyempurna pedoman rejimen ARV. Tujuan penelitian ini untuk mengetahui gambaran efektivitas terapi ARV paduan rejimen TLD (Tenofovir + Lamivudin + Dolutegravir) yang merupakan rejimen pilihan dan alternatif baru pada pasien HIV di RSUP Dr. Mohammad Hoesin Palembang. Analisis data dilakukan secara deskriptif dengan rancangan kohort retrospektif, menggunakan statistik univariat meliputi data karakteristik subjek dan efektivitas terapi ARV paduan rejimen TLD. Sampel penelitian ini terdiri dari 100 pasien HIV yang mulai menggunakan terapi ARV selama periode 1 April 2020 sampai dengan 30 September 2021. Kriteria inklusi dalam penelitian ini adalah pasien HIV baru yang mendapat terapi ARV paduan rejimen TLD dan berusia ≥18 tahun, sedangkan kriteria eksklusi adalah pasien HIV yang menggunakan terapi ARV paduan rejimen TLD mengganti paduan rejimen sebelum enam bulan terapi. Pasien HIV yang menggunakan paduan rejimen TLD pada penelitian ini lebih banyak berjenis kelamin laki-laki (77%), berusia produktif 20-49 tahun (89%), berpendidikan lulusan SLTA (51,8%), bekerja (68,9%), sudah menikah (48,5%) dan berasal dari luar kota Palembang (55%). Saat awal terdiagnosa HIV, pasien paling banyak pada stadium klinis satu (36,4%), status fungsional kerja (61%) dan ada infeksi oportunistik (61%). Hasil dari pemeriksaan viral load dan CD4 setelah enam bulan terapi yaitu lebih banyak pasien yang viral load < 200 kopi/ml (93,8%) dan kadar CD4 ≥ 200 mm3/sel (66,7%), hal ini menunjukkan bahwa terapi TLD efektif berdasarkan dari respon virologis dan imunologis yang baik. Namun dari hasil analisis hubungan karakteristik dengan viral load dan CD4, nilai P > 0,05 yang artinya karakteristik pasien tidak berpengaruh signifikan terhadap hasil viral load dan CD4 setelah enam bulan terapi.
Persepsi Pengguna terhadap Keberhasilan Implementasi SIMRS di Rawat Inap Rumah Sakit Santo Borromeus
Authors: Susanti Eni , Lazuardi Lutfan , Satibi Satibi 
Published: October 2024

Sistem Informasi Manajemen Rumah Sakit (SIMRS) terintegrasi di Rumah Sakit Santo Borromeus telah diterapkan sejak tahun 2019. Implementasi SIMRS ini sudah banyak dirasakan manfaatnya, namun masih ditemui permasalahan. Penelitian ini bertujuan untuk mendapatkan gambaran keberhasilan implementasi SIMRS di rawat inap RS Santo Borromeus. Jenis penelitian ini adalah deskriptif kuantitatif dengan desain cross sectional. Teknik pengumpulan data dengan survei melalui kuesioner. Populasi penelitian adalah seluruh pegawai RS Santo Borromeus dengan kriteria inklusi adalah petugas rumah sakit yang memiliki hak akses terhadap penggunaan SIMRS dan secara langsung menggunakan SIMRS di rawat inap dalam menunjang pekerjaan sehari-hari. Kriteria eksklusi adalah petugas rumah sakit yang tidak bersedia dilibatkan dalam penelitian dan dengan masa kerja kurang dari 1 tahun. Teknik pengumpulan data dengan proportionate stratified random sampling, sebanyak 204 responden terlibat dalam penelitian ini. Analisis data dilakukan secara deskriptif dengan cara menjelaskan destribusi prosentase dan rata-rata (mean) atas jawaban yang telah diberikan oleh responden. Hasil analisis menunjukkan tingkat keberhasilan implementasi SIMRS adalah tinggi dengan skor rata-rata kualitas sistem, kualitas informasi, kualitas layanan, penggunaan sistem, kepuasan pengguna, struktur, lingkungan, dan manfaat berturut-turut adalah 3,0; 2,9; 2,9; 3,2; 2,7; 3,1; 3,2; dan 3,0. Masih diperlukan pengembangan baik pada aspek teknologi, manusia, dan organisasi guna meningkatkan kualitas layanan pasien di rawat inap RS Santo Borromeus.Kata kunci: SIMRS; evaluasi sistem informasi; imlementasi SIMRS