bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transition from a Metal‐Localized Mixed‐Valence Compound to a Fully Delocalized and Bridge‐Biased Electrophore in a Ruthenium–Amine–Ruthenium Tricenter System
Authors: Tang Jian‐Hong , Shao Jiang‐Yang , He Yan‐Qin , Wu Si‐Hai , Yao Jiannian , Zhong Yu‐Wu 
Published: June 2016

AbstractA series of cyclometalated diruthenium complexes with a redox‐active amine bridge were synthesized. Depending on the terminal ligands of the ruthenium components and the substituent on the amine unit, the one‐electron‐oxidized state can be either in the form of a weakly or strongly coupled mixed‐valence diruthenium complex, a fully charge‐delocalized three‐center system, or a bridge‐biased electrophore. This transition among different electronic forms was supported by electrochemistry, near‐infrared absorption, electron paramagnetic resonance, and density functional theory analysis.
Optical Activity Governed by Local Chiral Structures in Two‐Dimensional Curved Metallic Nanostructures
Authors: Narushima Tetsuya , Hashiyada Shun , Okamoto Hiromi 
Published: June 2016

AbstractChiral nanostructures show macroscopic optical activity. Local optical activity and its handedness are not uniform in the nanostructure, and are spatially distributed depending on the shape of the nanostructure. In this study we fabricated curved chain nanostructures made of gold by connecting linearly two or more arc structures in a two‐dimensional plane. Spatial features of local optical activity in the chain structures were evaluated with near‐field circular dichroism (CD) imaging, and analyzed with the aid of classical electromagnetic simulation. The electromagnetic simulation predicted that local optical activity appears at inflection points where arc structures are connected. The handedness of the local optical activity was dependent on the handedness of the local chirality at the inflection point. Chiral chain structures have odd inflection points and the local optical activity distributed symmetrically with respect to structural centers. In contrast, achiral chain structures have even inflection points and showed antisymmetric distribution. In the near‐field CD images of fabricated chain nanostructures, the symmetric and antisymmetric distributions of local CD were observed for chiral and achiral chain structures, respectively, consistent with the simulated results. The handedness of the local optical activity was found to be determined by the handedness of the inflection point, for the fabricated chain structures having two or more inflection points. The local optical activity was thus governed primarily by the local chirality of the inflection points for the gold chain structures. The total effect of all the inflection points in the chain structure is considered to be a predominant factor that determines the macroscopic optical activity. Chirality 28:540–544, 2016. © 2016 Wiley Periodicals, Inc.
An intelligent system for fashion colour prediction based on fuzzy C‐means and gray theory
Authors: Hsiao Shih‐Wen , Lee Chu‐Hsuan , Chen Rong‐Qi , Yen Chih‐Huang 
Published: June 2016

AbstractFor design and manufacturing industries, to be able to capture the fashion trend is an essential factor that leads to winning a sale. However, colour predicting process in many organizations is not visible to the public. In order to provide colour trend to industries in advance, a predicting method is proposed in this study. In the method, the fuzzy c‐means was used to separate the collected colour data, then the minimum mean‐square error was used to place the similar colour clusters within different time point together and the gray model was adopted for prediction. In order to verify the prognostication of the system, four data announced by Pantone from spring 2014 to fall, 2015 were taken as the predicted samples and the colour for spring 2016 was predicted to compare with that in Pantone spring, 2016. The results show that the system has a high accuracy for predicting colour. The residual modified model constructed with the colour samples rearranged with MMSE has the best‐predicted result that ranged from 83.3% to 99.4%. It indicates that the result obtained with the rearranged samples is higher than that without rearrangement. Besides, the accuracy of the gray predicted results with residual modification would be more precise than the one without residual modification. Moreover, the value of mean squared error is quite low, which was ranged from 0.000025 to 0.0277. Therefore, the current intelligent predicting system satisfies the criteria of capturing colour in trend for enterprises. Moreover, it enables industries to make decisions for selecting the colour trend. © 2016 Wiley Periodicals, Inc. Col Res Appl, 42, 273–285, 2017
Use of In Situ Proximity Ligation Assays for Systems Analysis of Signaling Pathways
Authors: Chen Tzu‐Chi , Huang Chi‐Ying F. 
Published: June 2016

AbstractUnderstanding signaling pathway networks via protein‐protein interactions (PPIs) at the cellular level is a significant task that has not yet been completed. Here, a systems approach that computationally infers interlinked pathways from numerous PPIs is described. The endogenous PPIs can be empirically detected using an in situ proximity ligation assay (PLA), which detects and visualizes endogenous PPIs and post‐translational modifications of proteins with a high sensitivity and specificity. This unit includes two parts: (1) conversion of gene lists into PPIs for investigation and (2) large‐scale detection and analysis of endogenous PPIs for elucidating pathway networks. © 2016 by John Wiley & Sons, Inc.
The Influence of Divalent Anions on the Rectification Properties of Nanofluidic Diodes: Insights from Experiments and Theoretical Simulations
Authors: Pérez‐Mitta Gonzalo , Albesa Alberto G. , Toimil Molares María Eugenia , Trautmann Christina , Azzaroni Omar 
Published: June 2016

AbstractDuring the last decade, the possibility of generating synthetic nanoarchitectures with functionalities comparable to biological entities has sparked the interest of the scientific community related to diverse research fields. In this context, gaining fundamental understanding of the central features that determine the rectifying characteristics of the conical nanopores is of mandatory importance. In this work, we analyze the influence of mono‐ and divalent salts in the ionic current transported by asymmetric nanopores and focus on the delicate interplay between ion exclusion and charge screening effects that govern the functional response of the nanofluidic device. Experiments were performed using KCl and K2SO4 as representative species of singly and doubly charged species. Results showed that higher currents and rectification efficiencies are achieved by doubly charged salts. In order to understand the physicochemical processes underlying these effects simulations using the Poisson‐Nernst‐Planck formalism were performed. We consider that our theoretical and experimental account of the effect of divalent anions in the functional response of nanofluidic diodes provides further insights into the critical role of electrostatic interactions (ion exclusion versus charge screening effects) in presetting the ionic selectivity to anions as well as the observed rectification properties of these chemical nanodevices.
Development of a Representative Mouse Model with Nonalcoholic Steatohepatitis
Authors: Verbeek Jef , Jacobs Ans , Spincemaille Pieter , Cassiman David 
Published: June 2016

AbstractNon‐alcoholic fatty liver disease (NAFLD) is the most prevalent liver disease in the Western world. It represents a disease spectrum ranging from isolated steatosis to non‐alcoholic steatohepatitis (NASH). In particular, NASH can evolve to fibrosis, cirrhosis, hepatocellular carcinoma, and liver failure. The development of novel treatment strategies is hampered by the lack of representative NASH mouse models. Here, we describe a NASH mouse model, which is based on feeding non–genetically manipulated C57BL6/J mice a ‘Western style’ high‐fat/high‐sucrose diet (HF‐HSD). HF‐HSD leads to early obesity, insulin resistance, and hypercholesterolemia. After 12 weeks of HF‐HSD, all mice exhibit the complete spectrum of features of NASH, including steatosis, hepatocyte ballooning, and lobular inflammation, together with fibrosis in the majority of mice. Hence, this model closely mimics the human disease. Implementation of this mouse model will lead to a standardized setup for the evaluation of (i) underlying mechanisms that contribute to the progression of NAFLD to NASH, and (ii) therapeutic interventions for NASH. © 2016 by John Wiley & Sons, Inc.
Simple, High‐Yield Syntheses of DNA Duplexes Containing Interstrand DNA‐DNA Cross‐Links Between an N4‐Aminocytidine Residue and an Abasic Site
Authors: Gamboa Varela Jacqueline , Gates Kent S. 
Published: June 2016

AbstractThe protocol describes the preparation and purification of interstrand DNA‐DNA cross‐links derived from the reaction of an N
4‐aminocytidine residue with an abasic site in duplex DNA. The procedures employ inexpensive, commercially available chemicals and enzymes to carry out post‐synthetic modification of commercially available oligodeoxynucleotides. The yield of cross‐linked duplex is typically better than 90%. If purification is required, the cross‐linked duplex can be readily separated from single‐stranded DNA starting materials by denaturing gel electrophoresis. The resulting covalent hydrazone‐based cross‐links are stable under physiologically relevant conditions and may be useful for biophysical studies, structural analyses, DNA repair studies, and materials science applications. © 2016 by John Wiley & Sons, Inc.
Crumpled ZnMn2O4 Nanosheets for Long‐Term‐Cycling Lithium‐Ion Battery Anodes
Authors: Gu Shaozhen , Xu Jing , Lu Bingan 
Published: June 2016

AbstractCrumpled zinc manganate nanosheets (ZnMn2O4 NSs) have been synthesized by electrospinning and used as anode material for high‐performance lithium‐ion batteries. The crumpled ZnMn2O4 NSs exhibit a reversible capacity of 461 mAh g−1 at 100 mA g−1 even after 500 cycles, excellent rate capability, and long cycling stability (almost no capacity degradation after the 20th cycle). The excellent electrochemical performance may be mainly attributed to the novel crumpled nanostructure of the ZnMn2O4, which provides reduced diffusion length for the transport of Li+ ions as well as mechanical buffer space to accommodate the volume variation during Li+ insertion/extraction. The formation mechanism of electrospun crumpled ZnMn2O4 NSs is further discussed in detail.
Classification of Risks of Occupational Low Back Disorders with Support Vector Machines
Authors: Erdem Mehmet , Boran Fatih Emre , Akay Diyar 
Published: June 2016

AbstractThough technology has shown a rapid development, manual material handling (MMH) tasks are still usual activities in most industries. According to recent surveys, MMH tasks remain one of the main reasons for the emergence of occupational low back disorders (LBDs). It is critical to be able to discriminate as accurately as possible between MMH jobs that place workers at high versus low risk of LBDs. In this study, the risk of occupational LBDs has been classified by support vector machines (SVMs) considering both trunk motion variables and workplace variables, which have been extensively used to identify risk of LBDs. The LBDs‐SVM model has outperformed the existing models in terms of accuracy, which is equal to 88.5% for correct classification of high risk when 10‐cross validation is applied. In other words, the proposed model has correctly classified an average of 29.2 cases out of 33 high‐risk cases, which is critical to be determined compared to low‐risk cases. The results obtained in this study indicate that SVM is a better classifier than the other existing methods in the literature to classify LBDs risks.
Two approaches to the clinical dilemma of treating TTP with therapeutic plasma exchange in patients with a history of anaphylactic reactions to plasma
Authors: Sidhu Davinder , Snyder Edward L. , Tormey Christopher A. 
Published: June 2016

BackgroundThrombotic thrombocytopenic purpura (TTP) is a rare but serious disease caused by autoantibody‐mediated deficiency in von Willebrand factor (VWF) cleaving protease, ADAMTS‐13. The primary acute treatment is therapeutic plasma exchange (TPE). However, some patients can develop allergic/anaphylactic reactions to the replacement (i.e., donor) plasma over time. Two potential treatment strategies for patients with TTP who demonstrate severe allergic reactions to plasma used for exchange were examined.MethodsTwo patients with TTP exacerbations who developed severe allergic reactions to donor plasma were identified. One patient's TPE was re‐initiated with Octaplas, a lot‐batched solvent and detergent treated, type‐specific, pooled donor plasma product. The other patient was exchanged with primarily albumin, followed by slow incremental exposures to donor plasma to mitigate exposures and allergic risks. Both patients were assessed for anaphylaxis. Results: Both treatment strategies were successful in preventing any further clinically significant allergic/anaphylactic reactions and facilitated both patients’ TTP remissions.ConclusionsBased on our experience with two similar patients with TTP exacerbations and history of anaphylactic reactions to plasma during TPE, we have identified two possible treatment protocols to achieve remission in this clinical dilemma. Substituting Octaplas for standard plasma or, alternatively, using albumin with slowly increasing amounts of standard plasma may help to mitigate the risk of further anaphylactic adverse events. J. Clin. Apheresis 32:158–162, 2017. © 2016 Wiley Periodicals, Inc.
17β‐Estradiol Protects Skeletal Myoblasts From Apoptosis Through p53, Bcl‐2, and FoxO Families
Authors: La Colla Anabela , Vasconsuelo Andrea , Milanesi Lorena , Pronsato Lucía 
Published: June 2016

ABSTRACT17β‐Estradiol (E2) protects several nonreproductive tissues from apoptosis, including skeletal muscle. Previously, we showed that E2 at physiological concentrations prevented apoptosis induced by H2O2 in skeletal myoblasts, reverting PKCδ, JNK, and p66Shc activation and exerting a beneficial action over mitochondria. Since genomic actions underlying the regulation of nuclear gene transcription are a common property of this steroid, the present work characterizes the transcriptional activity modulated by E2 to exert its antiapoptotic effect. We report that E2 protects skeletal myoblasts against apoptosis induced by H2O2 modulating p53 and FoxO transcription factors and then their target genes Bcl‐2, Bim, Puma, PERP, and MDM2, without affecting Noxa gene. The results presented in this work support the notion that the transcription factors FoxO and p53 coordinate apoptosis in C2C12 cells, and deepens our knowledge about a putative molecular mechanism by which E2 exerts beneficial effects against oxidative stress in skeletal myoblasts. J. Cell. Biochem. 118: 104–115, 2017. © 2016 Wiley Periodicals, Inc.
The Effect of Hypoxia and Hyperoxia on Growth and Expression of Hypoxia‐Related Genes and Proteins in Spotted Gar Lepisosteus oculatus Larvae and Juveniles
Authors: Rimoldi Simona , Terova Genciana , Zaccone Giacomo , Parker Tim , Kuciel Michal , Dabrowski Konrad 
Published: June 2016

ABSTRACTWe studied the molecular responses to different water oxygen levels in gills and swim bladder of spotted gar (Lepisosteus oculatus), a bimodal breather. Fish at swim‐up stage were exposed for 71 days to normoxic, hypoxic, and hyperoxic water conditions. Then, all aquaria were switched to normoxic conditions for recovery until the end of the experiment (120 days). Fish were sampled at the beginning of the experiment, and then at 71 days of exposure and at 8 days of recovery.We first cloned three hypoxia‐related genes, hypoxia‐inducible factor 2α (HIF‐2α), Na+/H+ exchanger 1 (NHE‐1), and NHE‐3, and uploaded their cDNA sequences in the GeneBank database. We then used One Step Taqman® real‐time PCR to quantify the mRNA copies of target genes in gills and swim bladder of fish exposed to different water O2 levels. We also determined the protein expression of HIF‐2α and neuronal nitric oxide synthase (nNOS) in the swim bladder by using confocal immunofluorescence.Hypoxic stress for 71 days significantly increased the mRNA copies of HIF‐2α and NHE‐1 in gills and swim bladder, whereas normoxic recovery for 8 days decreased the HIF‐2α mRNA copies to control values in both tissues. We did not found significant changes in mRNA copies of the NHE‐3 gene in either gills or swim bladder in response to hypoxia and hyperoxia.Unlike in normoxic swim bladder, double immunohistochemical staining in hypoxic and hyperoxic swim bladder using nNOS/HIF‐2α showed extensive bundles of HIF‐2α‐positive nerve fibers in the trabecular musculature associated with a few varicose nNOS immunoreactive nerve terminals.
Catabolic effects of endothelial cell‐derived microparticles on disc cells: Implications in intervertebral disc neovascularization and degeneration
Authors: Pohl Pedro H.I. , Lozito Thomas P. , Cuperman Thais , Yurube Takashi , Moon Hong J. , Ngo Kevin , Tuan Rocky S. , St. Croix Claudette , Sowa Gwendolyn A. , Rodrigues Luciano M.R. , Kang James D. , Vo Nam V. 
Published: June 2016

ABSTRACTNeovascularization of intervertebral discs, a phenomenon considered pathological since normal discs are primarily avascular structures, occurs most frequently in annulus fibrosus (AF) of degenerated discs. Endothelial cells (ECs) are involved in this process, but the mechanism of the interaction between AF and endothelial cells is unclear. In this study, we evaluated the effects on matrix catabolic activity of AF cells by the extracellular endothelial microparticles (EMPs) and soluble protein factors (SUP fraction) produced from ECs. Passage 1 human AF cells grown in monolayer cultures were treated for 72 h with 250 µg of EMPs or SUP fraction isolated from culture of the microvascular endothelial cell line, HEMC‐I. Live‐cell imaging revealed uptake of EMPs by AF cells. RT‐PCR analysis demonstrated increased mRNA expression of MMP‐1 (50.3‐fold), MMP‐3 (4.5‐fold) and MMP‐13 (5.5‐fold) in AF cell cultures treated with EMPs compared to untreated control. Western analysis also demonstrated increased MMP protein expression in EMP‐treated AF cells. AF cells treated with the SUP fraction also exhibited a dramatic increase in MMP mRNA and protein expression. Increased MMP expression is primarily due to EMP or SUP stimulation of AF cells since EMPs or SUP fraction alone contained negligible amount of MMPs. Interestingly, MMP activity was elevated in AF cell cultures treated with EMPs but not with SUP. This study revealed enhanced matrix catabolism as a molecular consequence of action of ECs on AF cells via EMPs, which might be expected during neo‐angiogenesis of degenerating disc. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1466–1474, 2016.
Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples
Authors: Jiang Danni , Cao Biyun , Wang Meiyu , Yang Hong , Zhao Kang , Li Jianguo , Li Mingxin , Sun Lulu , Deng Anping 
Published: June 2016

AbstractBACKGROUNDAll β‐agonists are banned as feed additives for growth promotion in animals due to toxic effects on humans after consuming the β‐agonist contaminated meats. Phenylethanolamine A (PA) is a newly emerged β‐agonist. Thus there is a need to develop highly sensitive and specific analytical methods for the detection of PA in food samples. In this study, the monoclonal antibody (mAb) against PA was produced by hybridoma technology and used for the development of enzyme‐linked immunosorbent assay (ELISA).RESULTSThe IC50 values and limits of detection (LODs) of the ELISA using homogeneous combination of coating antigen/antibody for PA were 0.16 ng mL−1 and 0.011 ng mL−1, respectively. The cross‐reactive (CR) values of the assay with 14 structurally related β‐agonists were lower than 0.59%. Swine liver and meat samples were spiked with PA at different content and analysed by ELISA. Acceptable recovery rates of 91.40–105.51% and intra‐assay coefficients of variation of 1.56–9.92% (n = 3) were obtained. The ELISA for seven spiked samples was confirmed by LC‐MS/MS with a high correlation coefficient of 0.9881.CONCLUSIONThe proposed mAb‐based ELISA was highly sensitive and specific for PA and could be used as a quantitative/screening method for PA analysis in food samples. © 2016 Society of Chemical Industry
Influence of programming strain rates on the shape‐memory performance of semicrystalline multiblock copolymers
Authors: Yan Wan , Fang Liang , Noechel Ulrich , Kratz Karl , Lendlein Andreas 
Published: June 2016

ABSTRACTMultiblock copolymers named PCL‐PIBMD consisting of crystallizable poly(ε‐caprolactone) segments and crystallizable poly[oligo(3S‐iso‐butylmorpholine‐2,5‐dione)] segments coupled by trimethyl hexamethylene diisocyanate provide a versatile molecular architecture for achieving shape‐memory effects (SMEs) in polymers. The mechanical properties as well as the SME performance of PCL‐PIBMD can be tailored by the variation of physical parameters during programming such as deformation strain or applied temperature protocols. In this study, we explored the influence of applying different strain rates during programming on the resulting nanostructure of PCL‐PIBMD. Programming was conducted at 50 °C by elongation to εm = 50% with strain rates of 1 or 10 or 50 mm min−1. The nanostructural changes were visualized by atomic force microscopy (AFM) measurements and investigated by in situ wide and small angle X‐ray scattering experiments. With increasing the strain rate, a higher degree of orientation was observed in the amorphous domains. Simultaneously the strain‐induced formation of new PIBMD crystals as well as the fragmentation of existing large PIBMD crystals occurred. The observed differences in shape fixity ratio and recovery stress of samples deformed with various strain rates can be attributed to their different nanostructures. The achieved findings can be relevant parameters for programming the shape‐memory polymers with designed recovery forces. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2016, 54, 1935–1943
An innovative joint connecting beam for precast concrete shear wall structures
Authors: Lu Xilin , Wang Lu , Wang Dun , Jiang Huanjun 
Published: June 2016

AbstractThe precast shear wall structure has outstanding features for green buildings due to its construction convenience, safety, high quality and low pollution. In general, precast concrete shear walls are connected by multiple joints. The joint between precast walls has very strong influence on the whole structure, which calls for more detailed investigation. A new kind of connection – the joint connecting beam – was developed to connect the vertical reinforcement in precast concrete shear wall structures. This innovative connecting method has the advantages of convenient operation and saving steel. To evaluate the performance and for better application of the joint connecting beam, an experiment on seven full‐scale specimens was conducted under cyclic loading, including two cast‐in‐situ walls and five precast walls with varying reinforcement and sectional heights of the joint connecting beam. A comparison was performed between cast‐in‐situ walls and precast walls with joint connecting beam, focusing on failure mode, hysteretic curve, skeleton curve, bearing capacity, ductility and energy‐dissipating capacity. The results show that the joint connecting beam can effectively transfer the load of precast walls, especially for squat precast walls. Moreover, finite element models were developed to simulate the performance of the specimens. The simulation results agree well with experimental results.