bluuebunny/crossref_metadata_2025_split huggingface dataset:
تأثير تمرينات تعليمية بوسائل مساعدة في بعض القدرات الحركية للمبارزة بسلاح الشيش للطلاب
Authors: Taiba Faisal Jaber  , Prof. Dr. Anis Hussein Ali  , Asst. Prof. Dr. Issam Talib Abbas  
Published: September 2024

تعد رياضة المبارزة إحدى الألعاب التي لها متطلباتها وأهدافها الرياضية والاجتماعية والنفسية والتربوية حيث تمارس على وفق قوانين معينة ومسارات حركية وقواعد تنظيمية خاصة تتطلب من ممارسيها بعض المتغيرات البدنية والحركية الخاصة والمميزة لكي يحافظ اللاعب فيها على مستواه البدني والحركي ويتمكن من التقدم الفني باتجاه الأداء المهاري الأمثل للوصول إلى أفضل النتائج صيغت مشكلة البحث من خلال الباحثون على العينة باعتبارها احد مدربين الفئات العمرية التي تشرف على فئة الشباب لاحظت هؤلاء اللاعبين وصلوا الى مرحلة عدم التطور لذلك ارتأت الباحثون باستعمال وسائل مساعدة مختلفة حتى تغير من النمط الذي كان يستخدم لهم بالتعلم حتى تطور القدرات الحركية والاداء المهارى لديهم ، حيث يهدف البحث الى تأثير التمرينات التعليمية بوسائل مساعدة في بعض القدرات الحركية للمبارزة بسلاح الشيش للطلاب. استخدم الباحثون المنهج التجريبي بتصميم المجموعتين المتكافئتين لملائمة وطبيعة المشكلة تحدد مجتمع البحث بطلاب المرحلة الثالثة كلية التربية البدنية وعلوم الرياضة للعام الدراسي (2023-2024) والبالغ عددهم (24 طالب) عمدت الباحثون تقسيمهم الى مجموعتين بالطريقة العشوائية البسيطة.
Engineered and hybrid human megakaryocytic extracellular vesicles for targeted non-viral cargo delivery to hematopoietic (blood) stem and progenitor cells
Authors: Das Samik , Thompson Will , Papoutsakis Eleftherios Terry 
Published: September 2024

Native and engineered extracellular vesicles generated from human megakaryocytes (huMkEVs) or from the human megakaryocytic cell line CHRF (CHEVs) interact with tropism delivering their cargo to both human and murine hematopoietic stem and progenitor cells (HSPCs). To develop non-viral delivery vectors to HSPCs based on MkEVs, we first confirmed, using NOD-scid IL2Rγnull (NSG™) mice, the targeting potential of the large EVs, enriched in microparticles (huMkMPs), chosen for their large cargo capacity. 24 h post intravenous infusion into NSG mice, huMkEVs induced a nearly 50% increase in murine platelet counts. PKH26-labeled huMkEVs or CHEVs localized to the HSPC-rich bone marrow preferentially interacting with murine HSPCs, thus confirming their receptor-mediated tropism for NSG HSPCs, and their potential to treat thromobocytopenias. We explored this tropism to functionally deliver synthetic cargo, notably plasmid DNA coding for a fluorescent reporter, to NSG HSPCs both in vitro and in vivo. We loaded huMkEVs with plasmid DNA either through electroporation or by generating hybrid particles with preloaded liposomes. Both methods facilitated successful functional targeted delivery of pDNA, as tissue weight-normalized fluorescence intensity of the expressed fluorescent reporter was significantly higher in bone marrow than other tissues. Furthermore, the fraction of fluorescent CD117+ HSPCs was nearly 19-fold higher than other cell types within the bone marrow 72-h following administration of the hybrid particles, further supporting that HSPC tropism is retained when using hybrid particles. These data demonstrate the potential of these EVs as a non-viral, HSPC-specific cargo vehicle for gene therapy applications to treat hematological diseases.
Comprehensive metabolomics and transcriptomics analyses investigating the regulatory effects of different sources of dietary astaxanthin on the antioxidant and immune functions of commercial-sized rainbow trout
Authors: Chen Li , Wang Lei , Li Yaopeng , Wu Xugan , Long Xiaowen 
Published: September 2024

Astaxanthin is an important aquatic feed additive that enhances the antioxidant capacity, and immune function of rainbow trout (Oncorhynchus mykiss); however, very limited information is available on its underlying molecular mechanisms. Haematococcus pluvialis powder, Phaffia rhodozyma powder, and synthetic astaxanthin were added to the commercial feed (no astaxanthin, NA) to prepare three experimental feeds, referred to as the HPA, PRA, and SA groups, respectively, and their actual astaxanthin contents were 31.25, 32.96, and 31.50 mg.kg-1, respectively. A 16-week feeding trial was conducted on the O. mykiss with an initial body weight of 669.88 ± 36.22 g. Serum and head kidney samples from commercial-sized O. mykiss were collected for metabolomics and transcriptomics analysis, respectively. Metabolomics analysis of the serum revealed a total of 85 differential metabolites between the astaxanthin-supplemented group and the control group. These metabolites were involved in more than 30 metabolic pathways, such as glycerophospholipid metabolism, fatty acid biosynthesis, linoleic acid metabolism, and arginine and proline metabolism. It is speculated that different sources of dietary astaxanthin may regulate antioxidant capacity and immunity mainly by affecting lipid metabolism and amino acid metabolism. Transcriptomic analysis of the head kidney revealed that the differentially expressed genes between the astaxanthin-supplemented group and the control group, such as integrin beta-1 (ITGB1), alpha-2-macroglobulin (A2M), diamine acetyltransferase 1 (SAT1), CCAAT/enhancer-binding protein beta (CEBPB) and DNA damage-inducible protein 45 alpha (GADD45A), which are involved in cell adhesion molecules, the FoxO signaling pathway, phagosomes, and arginine and proline metabolism and play regulatory roles in different stages of the antioxidant and immune response of O. mykiss.
Spatiotemporal patterns of rheumatic heart disease burden attributable to high systolic blood pressure, high sodium diet, and lead exposure (1990 to 2019): a longitudinal observational study
Authors: Zhang Yanli , Zhang Jun , Liu Yonggang , Zhou Yuzhe , Ye Lu , Chen Kaiming , Jiao Jinghua 
Published: September 2024

BackgroundRheumatic heart disease (RHD) continues to be a significant global health concern, exhibiting unique regional disparities. Although there is a noted decline in the burden of RHD, the specific causatives for this decrease remain unclear. This study aims to identify and quantify the spatiotemporal patterns related to the RHD-attributable risk burden.MethodsThe data pertaining to deaths and disability-adjusted life years (DALYs) attributable to RHD risk were drawn from the Global Burden of Disease (GBD) study conducted from 1990 to 2019. These data, categorized by age, gender, and geographical location, highlighted risk factors including diets high in sodium, elevated systolic blood pressure (SBP), and lead exposure. To examine the long-term trends in RHD changes due to these specific risk factors, the average annual percentage change (AAPC) method was used.ResultsDuring the past 30 years, the highest decrease in RHD burden was attributed to high SBP. An AAPC of −2.73 [95% confidence interval (CI): −2.82 to −2.65] and − 2.45 (95% CI: −2.55 to −2.36) in deaths and DALYs was attributable to high SBP, while an AAPC of −3.99 (95% CI: −4.14 to −3.85) and − 3.74 (95% CI: −3.89 to −3.6) in deaths and DALYs was attributed to a diet high in sodium. Moreover, the trends in deaths and DALYs due to lead exposure also showed decreases with an AAPC of −2.94 (95% CI: −3 to −2.89) and − 3.46 (95% CI: −3.58 to −3.34) from 1990 to 2019. Oceania showed an upward trend of the RHD DALYs due to high SBP, with an AAPC of 0.23 (95% CI: 0.13 to 0.33). In general, countries in Oceania, East Asia, and South Asia had higher age-standard deaths and DALY rates of RHD due to diets high in sodium.ConclusionOur study has revealed that high SBP remains the prime risk factor contributing to the RHD burden. There are decreasing spatiotemporal patterns in RHD-related deaths and burdens. Gaining this knowledge is fundamental to making informed public health strategies and clinical decisions, especially concerning risk assessment, screening, and prevention initiatives.
Sex differences in nicotine intake and relapse behavior in nicotine-dependent adult wistar rats
Authors: Chellian Ranjithkumar , Behnood-Rod Azin , Bruijnzeel Adriaan W. 
Published: September 2024

IntroductionTobacco use is highly addictive and the leading cause of premature mortality in the world. Long-access nicotine self-administration procedures in rats closely model human smoking behavior. However, significant gaps remain in our understanding of sex differences in the development of dependence and relapse in adult rats.MethodsIn the present study, we investigated operant responding for both nicotine and saline and the development of dependence in adult rats of both sexes. The rats had daily access to nicotine or saline for 6 h per day, 7 days per week. Dependence was assessed by evaluating precipitated and spontaneous somatic withdrawal signs, measuring locomotor activity in the small open field test, and assessing anxiety-like behavior in the large open field and elevated plus maze test. The sucrose preference test was used to determine if cessation of nicotine intake leads to anhedonia. It was also investigated if a period of forced abstinence affects nicotine-seeking behavior.ResultsThis study showed that nicotine intake is higher in females than in males when given daily long access to nicotine. Daily nicotine self-administration led to more precipitated and spontaneous somatic withdrawal signs compared to saline self-administration, with no sex differences observed. In addition, cessation of nicotine intake led to a similar increase in activity in both males and females in the small open field test. However, cessation of nicotine intake did not increase anxiety-like behavior or cause anhedonia in either males or females. A time course analysis revealed that the nicotinic acetylcholine receptor antagonist mecamylamine affected nicotine intake differently in males and females, increasing intake in males and decreasing intake in females. Three weeks of forced abstinence led to an increase in nicotine and saline-seeking behavior. The rats exhibited more nicotine than saline seeking, and the females displayed more nicotine seeking than the males.DiscussionThe present findings demonstrate that females self-administer more nicotine and display more nicotine-seeking behavior than males. Furthermore, there were no sex differences in somatic withdrawal signs or activity during abstinence from nicotine. This work underscores the importance of considering sex differences across various aspects of addiction, including intake and relapse, when developing novel treatments for tobacco use disorder.
Do gifted children without specific learning disabilities read more efficiently than typically developing children?
Authors: Lesecq Laurent , Querne Laurent , Gornes Julie , Buffo Laura , Corbel Louise , Le Moing Anne Gaelle , Berquin Patrick , Bourdin Béatrice 
Published: September 2024

IntroductionThere are no published data on the written language skills of gifted children (GC). The objective of the present study was to evaluate reading abilities of GC vs. normative data from typically developing French children (TDC). Like English, French is considered to be an opaque language.MethodGC completed the Wechsler Intelligence Scales and a battery of language tests. Only children with a score two standard deviations (SD) above the norm were included. GC with current or past academic difficulties or specific learning disorders were excluded. The GC’s scores were compared with TDC’s normative scores for language tests in a chi-square-test and corrected for multiple comparisons.ResultsForty-five GC were included. The highest GC’s mean scores were for the WISC’s Verbal Comprehension Index (VCI) and the lowest for the Processing Speed Index (from more than two SDs to one SD higher above the TDC’s normative scores). GC were between 1.3 and 4.7 times more likely than TDC to achieve a high score. After correction, the distributions of the GC’s and TDC’s scores differed significantly with regard to spoonerism, phoneme deletion, and rapid automatic naming (p &lt; 0.001), word and sentence repetition (p ≤ 0.007), and the reading of meaningful text (p = 0.03). GC and TDC did not differ significantly for reading meaningless texts and spelling accuracy.DiscussionAs described in the literature, the GC in the present study had heterogeneous scores on the Wechsler Intelligence Scales. The GC performed better than TDC in assessments of the underlying skills of reading and when reading of meaningful texts. This advantage was lost in the absence of context, as shown by the lack of significant GC vs. TDC differences for reading meaningless texts and for spelling accuracy. Hence, GC presented a heterogeneous profile with regard to the underlying skills of reading and reading abilities. The present data should help to improve our understanding of GC’s reading skills. In particular, it is now essential to determine which written language tests and which score thresholds are appropriate for identifying specific learning disorders in GC.
Prevalence and Molecular Epidemiology of Intestinal Colonization by Multidrug-Resistant Bacteria among Hematopoietic Stem-Cell Transplantation Recipients: A Bulgarian Single-Center Study
Authors: Niyazi Denis , Vergiev Stoyan , Markovska Rumyana , Stoeva Temenuga 
Published: September 2024

Background/Objectives: Intestinal colonization by multidrug-resistant (MDR) bacteria is considered one of the main risk factors for invasive infections in the hematopoietic stem-cell transplant (HSCT) setting, associated with hard-to-eradicate microorganisms. The aim of this study was to assess the rate of intestinal colonization by MDR bacteria and their microbial spectrum in a group of post-HSCT patients to study the genetic determinants of beta-lactam and glycopeptide resistance in the recovered isolates, as well as to determine the epidemiological relation between them. Methods: The intestinal colonization status of 74 patients admitted to the transplantation center of University Hospital “St. Marina”—Varna in the period January 2019 to December 2021 was investigated. Stool samples/rectal swabs were screened for third-generation cephalosporin and/or carbapenem-resistant Gram-negative bacteria, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and Stenotrophomonas maltophilia. Identification and antimicrobial susceptibility testing were performed by Phoenix (BD, Sparks, MD, USA) and MALDI Biotyper sirius (Bruker, Bremen, Germany). Molecular genetic methods (PCR, DNA sequencing) were used to study the mechanisms of beta-lactam and glycopeptide resistance in the collected isolates, as well as the epidemiological relationship between them. Results: A total of 28 patients (37.8%) were detected with intestinal colonization by MDR bacteria. Forty-eight non-duplicate MDR bacteria were isolated from their stool samples. Amongst them, the Gram-negative bacteria prevailed (68.8%), dominated by ESBL-producing Escherichia coli (30.3%), and followed by carbapenem-resistant Pseudomonas sp. (24.2%). The Gram-positive bacteria were represented exclusively by Enterococcus faecium (31.2%). The main beta-lactam resistance mechanisms were associated with CTX-M and VIM production. VanA was detected in all vancomycin-resistant enterococci. A clonal relationship was observed among Enterobacter cloacae complex and among E. faecium isolates. Conclusions: To the best of our knowledge, this is the first Bulgarian study that presents detailed information about the prevalence, resistance genetic determinants, and molecular epidemiology of MDR gut-colonizing bacteria in HSCT patients.
Methanolic Extract of Cimicifuga foetida Induces G1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells
Authors: Chang Chih-Hsuan , Tsai Hung-Pei , Yen Ming-Hong , Lin Chien-Ju 
Published: September 2024

Background: Glioblastoma multiforme (GBM) is among the most aggressive and challenging brain tumors, with limited treatment options. Cimicifuga foetida, a traditional Chinese medicine, has shown promise due to its bioactive components. This study investigates the anti-glioma effects of a methanolic extract of C. foetida (CF-ME) in GBM cell lines. Methods: The effects of CF-ME and its index compounds (caffeic acid, cimifugin, ferulic acid, and isoferulic acid) on GBM cell viability were assessed using MTT assays on U87 MG, A172, and T98G cell lines. The ability of CF-ME to induce cell cycle arrest, apoptosis, and autophagy and inhibit metastasis was evaluated using flow cytometry, Western blotting, and functional assays. Additionally, the synergistic potential of CF-ME with temozolomide (TMZ) was explored. Results: CF-ME significantly reduced GBM cell viability in a dose- and time-dependent manner, induced G1 phase cell cycle arrest, promoted apoptosis via caspase activation, and triggered autophagy. CF-ME also inhibited GBM cell invasion, migration, and adhesion, likely by modulating epithelial–mesenchymal transition (EMT) markers. Combined with TMZ, CF-ME further enhanced reduced GBM cell viability, suggesting a potential synergistic effect. However, the individual index compounds of CF-ME exhibited only modest inhibitory effects, indicating that the full anti-glioma activity may result from the synergistic interactions among its components. Conclusions: CF-ME exhibited potent anti-glioma activity through multiple mechanisms, including cell cycle arrest, apoptosis, autophagy, and the inhibition of metastasis. Combining CF-ME with TMZ further enhanced its therapeutic potential, making it a promising candidate for adjuvant therapy in glioblastoma treatment.
The role of Islamic tolerance in ensuring socio-political stability in Uzbekistan
Authors: Farfiev B. A. 
Published: September 2024

According to the author, it is known from the history of mankind that rational relationships between members of society are necessary for the well-being of people, the development of society. In order to maintain a stable state of society, the power structures of the state play a significant role, but spiritual factors, including religion, play a significant role in this process. But not in all countries of the Muslim world, especially in the Arab states, the tolerant quality of Islam is applied in interethnic and interfaith relations. Although, as many believe, the Qur’an is sent down in Arabic, the hadiths are also in that language. However, people who speak Arabic do not fully comply with Islam’s demands for tolerance towards non-Arabic speakers in everyday and public life. The article substantiates these conclusions with examples from the public life of Middle Eastern countries. Based on the results of comparative analysis, the author concludes that in Uzbekistan, among the spiritual factors that served as the basis for ensuring and strengthening socio-political stability, the importance of Islam and its principle of tolerance are manifested at a special level. In theoretical and practical aspects the role of the principle of Islamic tolerance as a factor in ensuring and strengthening socio-political stability of Uzbekistan is investigated.
Efektivitas Undang-Undang No. 12 Tahun 2022 dalam Mencegah Kasus Kekerasan dan Pelecehan Seksual di Kota Bandung
Authors: Aprilia Erry 
Published: September 2024

In Indonesia, efforts related to the launch and adoption of e-Governance initiatives have been made in various fields. However, so far most of these efforts have not achieved the expected results over a longer period. This has led to underutilization of public funds. In addition, there are variations in the adoption and success of e-Governance initiatives across Indonesia, mainly due to prevalent diversity. The implementation of good governance principles in BPJS Health services was measured based on a sample of 50 respondents of BPJS Health service users at Cinunuk Health Center, then analyzed using the Rasch Model. The method applied in this research is a quantitative approach, so the application of nonexperimental is used in this research. Primary data was obtained through a questionnaire design and distributed online with the help of the google form application. The purpose of this study is to identify the factors responsible for higher adoption of e-Governance services in Indonesia. This research will also analyze the impact of the ongoing pandemic on e-Governance adoption by including a separate construct related to healthcare.

ABSTRAK

Di Indonesia, upaya terkait peluncuran dan adopsi inisiatif e-Governance telah dilakukan dalam berbagai bidang. Namun, sejauh ini sebagian besar upaya tersebut belum mencapai hasil yang diharapkan dalam periode yang lebih panjang. Hal ini menyebabkan kurangnya pemanfaatan dana publik. Selain itu, terdapat variasi dalam adopsi dan keberhasilan inisiatif e-Governance di seluruh wilayah Indonesia, terutama karena adanya keragaman yang prevalen. Implementasi prinsip good governance dalam pelayanan BPJS Kesehatan diukur berdasarkan sampel sebanyak 50 responden para pengguna layanan BPJS Kesehatan di Puskesmas Cinunuk, lalu dianalisis menggunakan Model Rasch. Metode yang diaplikasikan dalam penelitian ini ialah pendekatan kuantitatif, sehingga penerapan nonexperimental digunakan dalam penelitian ini. Data primer diperoleh melalui rancangan kuesioner dan disebarkan secara online dengan bantuan aplikasi google form. Tujuan dari penelitian ini adalah untuk mengidentifikasi faktor-faktor yang bertanggung jawab atas adopsi layanan e-Governance yang lebih tinggi di Indonesia. Penelitian ini juga akan menganalisis dampak pandemi yang sedang berlangsung terhadap adopsi e-Governance dengan memasukkan konstruk terpisah yang terkait dengan pelayanan kesehatan.
Analisis Implementasi Prinsip Good Governance dalam Pelayanan Bpjs Kesehatan (Studi Kasus di Puskesmas Cinunuk)
Authors: Aprilia Erry 
Published: September 2024

In Indonesia, efforts related to the launch and adoption of e-Governance initiatives have been made in various fields. However, so far most of these efforts have not achieved the expected results over a longer period. This has led to underutilization of public funds. In addition, there are variations in the adoption and success of e-Governance initiatives across Indonesia, mainly due to prevalent diversity. The implementation of good governance principles in BPJS Health services was measured based on a sample of 50 respondents of BPJS Health service users at Cinunuk Health Center, then analyzed using the Rasch Model. The method applied in this research is a quantitative approach, so the application of nonexperimental is used in this research. Primary data was obtained through a questionnaire design and distributed online with the help of the google form application. The purpose of this study is to identify the factors responsible for higher adoption of e-Governance services in Indonesia. This research will also analyze the impact of the ongoing pandemic on e-Governance adoption by including a separate construct related to healthcare.

ABSTRAK

Di Indonesia, upaya terkait peluncuran dan adopsi inisiatif e-Governance telah dilakukan dalam berbagai bidang. Namun, sejauh ini sebagian besar upaya tersebut belum mencapai hasil yang diharapkan dalam periode yang lebih panjang. Hal ini menyebabkan kurangnya pemanfaatan dana publik. Selain itu, terdapat variasi dalam adopsi dan keberhasilan inisiatif e-Governance di seluruh wilayah Indonesia, terutama karena adanya keragaman yang prevalen. Implementasi prinsip good governance dalam pelayanan BPJS Kesehatan diukur berdasarkan sampel sebanyak 50 responden para pengguna layanan BPJS Kesehatan di Puskesmas Cinunuk, lalu dianalisis menggunakan Model Rasch. Metode yang diaplikasikan dalam penelitian ini ialah pendekatan kuantitatif, sehingga penerapan nonexperimental digunakan dalam penelitian ini. Data primer diperoleh melalui rancangan kuesioner dan disebarkan secara online dengan bantuan aplikasi google form. Tujuan dari penelitian ini adalah untuk mengidentifikasi faktor-faktor yang bertanggung jawab atas adopsi layanan e-Governance yang lebih tinggi di Indonesia. Penelitian ini juga akan menganalisis dampak pandemi yang sedang berlangsung terhadap adopsi e-Governance dengan memasukkan konstruk terpisah yang terkait dengan pelayanan kesehatan