bluuebunny/crossref_metadata_2025_split huggingface dataset:
Actions in response to drug safety signals arising from a spontaneous reporting system: Retrospective study in The Netherlands
Authors: Rolfes Leàn , Kolfschoten Judith , van Hunsel Florence , Kooijman Michel , van Puijenbroek Eugène 
Published: August 2016

BACKGROUND: There is limited information on actions taken in response to drug safety signals originating from a spontaneous reporting system (SRS) in pharmacovigilance. In The Netherlands the Pharmacovigilance Centre Lareb is an independent organization that works in close collaboration with the Dutch regulatory agency, the Medicines Evaluation Board (MEB). OBJECTIVE: The objective of this study is to gain insight in steps undertaken on signals originating from the SRS and disseminated by Lareb from 2008–2012. METHOD: For all signals the recommendations of Lareb and the following steps proposed by the MEB were analyzed. Secondary outcomes were prioritization of the signal, the year of dissemination and if Lareb published (inter)nationally about the signal. Pearson’s Chi-square (X2) and Mantel-Haenszel statistics were used for statistical analysis. RESULTS: Of all signals disseminated by Lareb from 2008–2012, 90,7% resulted in an action: in 87% a regulatory action and in 36% an (inter)national publication. Generally, Lareb’s recommendations correspond to steps undertaken by the MEB. CONCLUSION: This study found influence of signal prioritization on Lareb’s recommendations but not on the steps undertaken by the MEB. Trends over time were only seen for steps undertaken by the MEB. These differences are most probably due to responsibilities of the different EU member states of various drugs.
Simultaneous analysis of defense‐related phytohormones in Arabidopsis thaliana responding to fungal infection
Authors: Riet Katlego B. , Ndlovu Nombuso , Piater Lizelle A. , Dubery Ian A. 
Published: August 2016

Premise of the study:Simultaneous analysis of defense‐related phytohormones can provide insights into underlying biochemical interactions. Ultra‐high‐performance liquid chromatographic (UHPLC) techniques hyphenated to electrospray ionization mass spectrometry (ESI‐MS) are powerful analytical platforms, suitable for quantitative profiling of multiple classes of metabolites.Methods:An efficient and simplified extraction method was designed followed by reverse‐phase UHPLC for separation of seven phytohormones: salicylic acid, methyl salicylate, jasmonic acid, methyl jasmonate, absiscic acid, indole acetic acid, and the ethylene precursor 1‐aminocyclopropane‐1‐carboxylic acid. A triple quadrupole multiple reaction monitoring (MRM) method was developed for MS quantification. The methods were applied to analyze phytohormones in Arabidopsis leaf tissue responding to biotic stresses.Results:Under the optimized conditions, the phytohormones were separated within 15 min, with good linearities and high sensitivity. Repeatable results were obtained, with the limits of detection and quantification around 0.01 ng/μL (∼9 ng/g tissue). The method was validated and applied to monitor, quantify, and compare the temporal changes of the phytohormones under biotic stress.Discussion:Quantitative changes indicate increased production of defense phytohormones from the various classes. The analytical method was useful and suitable to distinguish distinctive variations in the phytohormonal profiles and balance in A. thaliana leaves resulting from pathogen attack.
REDEFINING THE BOUNDARIES OF INTERPLANETARY CORONAL MASS EJECTIONS FROM OBSERVATIONS AT THE ECLIPTIC PLANE
Authors: Cid C. , Palacios J. , Saiz E. , Guerrero A. 
Published: August 2016

ABSTRACT
On 2015 January 6–7, an interplanetary coronal mass ejection (ICME) was observed at L1. This event, which can be associated with a weak and slow coronal mass ejection, allows us to discuss the differences between the boundaries of the magnetic cloud and the compositional boundaries. A fast stream from a solar coronal hole surrounding this ICME offers a unique opportunity to check the boundaries’ process definition and to explain differences between them. Using Wind and ACE data, we perform a complementary analysis involving compositional, magnetic, and kinematic observations providing relevant information regarding the evolution of the ICME as travelling away from the Sun. We propose erosion, at least at the front boundary of the ICME, as the main reason for the difference between the boundaries, and compositional signatures as the most precise diagnostic tool for the boundaries of ICMEs.
CARBON STARS IN THE SATELLITES AND HALO OF M31
Authors: Hamren Katherine , Beaton Rachael L. , Guhathakurta Puragra , Gilbert Karoline M. , Tollerud Erik J. , Boyer Martha L. , Rockosi Constance M. , Smith Graeme H. , Majewski Steven R. , Howley Kirsten 
Published: August 2016

ABSTRACT
We spectroscopically identify a sample of carbon stars in the satellites and halo of M31 using moderate-resolution optical spectroscopy from the Spectroscopic and Photometric Landscape of Andromeda’s Stellar Halo survey. We present the photometric properties of our sample of 41 stars, including their brightness with respect to the tip of the red giant branch (TRGB) and their distributions in various color–color spaces. This analysis reveals a bluer population of carbon stars fainter than the TRGB and a redder population of carbon stars brighter than the TRGB. We then apply principal component analysis to determine the sample’s eigenspectra and eigencoefficients. Correlating the eigencoefficients with various observable properties reveals the spectral features that trace effective temperature and metallicity. Putting the spectroscopic and photometric information together, we find the carbon stars in the satellites and halo of M31 to be minimally impacted by dust and internal dynamics. We also find that while there is evidence to suggest that the sub-TRGB stars are extrinsic in origin, it is also possible that they are are particularly faint members of the asymptotic giant branch.
LAMOST OBSERVATIONS IN THE KEPLER FIELD. ANALYSIS OF THE STELLAR PARAMETERS MEASURED WITH LASP BASED ON LOW-RESOLUTION SPECTRA*
Authors: Ren Anbing , Fu Jianning , Cat Peter De , Wu Yue , Yang Xiaohu , Shi Jianrong , Luo Ali , Zhang Haotong , Dong Subo , Zhang Ruyuan , Zhang Yong , Hou Yonghui , Wang Yuefei , Cao Zihuang , Du Bing 
Published: August 2016

ABSTRACT
All 14 subfields of the Kepler field were observed at least once with the Large Sky Area Multi-Object Fiber Spectroscopic Telescope (lamost, Xinglong Observatory, China) during the 2012–2014 observation seasons. There are 88,628 reduced spectra with a signal-to-noise ratio in the g band (S/N
g
) ≥ 6 after the first round (2012–2014) of observations of the lamost–
Kepler project (LK-project). By adopting the upgraded version of the lamost Stellar Parameter pipeline (lasp), we have determined the atmospheric parameters (

,

, and [Fe/H]) and heliocentric radial velocity v
rad for 51,406 stars with 61,226 spectra. Compared with the atmospheric parameters derived from both high-resolution spectroscopy and asteroseismology for common stars in Huber et al., an external calibration of lasp atmospheric parameters was made, leading to the determination of the external errors for giants and dwarfs. Multiple spectroscopic observations of the same objects in the LK-project were used to estimate the internal uncertainties of the atmospheric parameters as a function of S/N
g
with the unbiased estimation method. The lasp atmospheric parameters were calibrated based on both the external and internal uncertainties for the giants and dwarfs. A general statistical analysis of the stellar parameters leads to the discovery of 106 candidate metal-poor stars, 9 candidate very metal-poor stars, and 18 candidate high-velocity stars. Fitting formulae were obtained segmentally for both the calibrated atmospheric parameters of the LK-project and the Kepler Input Catalog (KIC) parameters with common stars. The calibrated atmospheric parameters and radial velocities of the LK-project will be useful for studying stars in the Kepler field.
SPECTRAL PROPERTIES OF COOL STARS: EXTENDED ABUNDANCE ANALYSIS OF 1,617 PLANET-SEARCH STARS
Authors: Brewer John M. , Fischer Debra A. , Valenti Jeff A. , Piskunov Nikolai 
Published: August 2016

ABSTRACT
We present a catalog of uniformly determined stellar properties and abundances for 1,617 F, G, and K stars using an automated spectral synthesis modeling procedure. All stars were observed using the HIRES spectrograph at Keck Observatory. Our procedure used a single line list to fit model spectra to observations of all stars to determine effective temperature, surface gravity, metallicity, projected rotational velocity, and the abundances of 15 elements (C, N, O, Na, Mg, Al, Si, Ca, Ti, V, Cr, Mn, Fe, Ni, and Y). Sixty percent of the sample had Hipparcos parallaxes and V-band photometry, which we combined with the spectroscopic results to obtain mass, radius, and luminosity. Additionally, we used the luminosity, effective temperature, metallicity and α-element enhancement to interpolate in the Yonsei–Yale isochrones to derive mass, radius, gravity, and age ranges for those stars. Finally, we determined new relations between effective temperature and macroturbulence for dwarfs and subgiants. Our analysis achieved precisions of 25 K in

, 0.01 dex in [M/H], 0.028 dex for

, and 0.5 km s−1 in

based on multiple observations of the same stars. The abundance results were similarly precise, between ∼0.01 and ∼0.04 dex, though trends with respect to

remained for which we derived empirical corrections. The trends, though small, were much larger than our uncertainties and are shared with published abundances. We show that changing our model atmosphere grid accounts for most of the trend in [M/H] between 5000 and 5500 K, indicating a possible problem with the atmosphere models or opacities.
Cinema nacional: o universo do trabalho em “Mauá: o imperador e o rei”
Authors: Fanini Angela Maria Rubel 
Published: August 2016

<p>Neste artigo analisamos como ocorre certa formalização ideológica do universo do trabalho no filme <strong>“</strong>Mauá<strong>: </strong>O Imperador e o Rei”, produção executiva de Joaquim Vaz de Carvalho e direção de Sérgio Rezende. Várias representações do trabalho (derrogatório, material, imaterial, dignificante, fonte de progresso, riqueza e valor social e pessoal) são destacadas uma vez que a atividade laboral se dá em um contexto social brasileiro bastante complexo cujo cenário apresenta posições sociais e econômicas antagônicas entre dois pólos: idéias e práticas liberais vinculadas a valores burgueses e conservadoras atreladas a uma economia predominantemente agro-exportadora dependente da mão-de-obra escrava.</p>
A imprensa do Paraná nos anos de redemocratização do Brasil – proposta para investimento em pesquisa
Authors: Marinho José Aparecido 
Published: August 2016

<span>Este artigo propõe o investimento, como fonte de estudo e pesquisa, da participação da imprensa paranaense, por meio dos principais jornais do Estado, no processo de construção da história da comunicação, pelo viés do tratamento dispensado à cobertura dos fatos que marcaram a condução da política nacional a partir do final do governo militar, em 1985, abrangendo um período de 20 anos da retomada da democracia no País. </span>
Qual é a graça? O humor como estratégia de liberdade na sociedade contemporânea
Authors: Demozzi Sabrina 
Published: August 2016

<p>O presente artigo busca debater algumas manifestações do humor no jornalismo brasileiro a partir de características conceituais como o jornalismo alternativo, a carnavalização pelo humor e o chiste. A partir da ideia do humor como ação libertária para o questionamento da autoridade e dos discursos de verdade, busca-se dialogar com conceitos como o de vida-artista proposto por Michel Foucault, para quem a autonomia do pensamento seria uma estratégia de liberdade na sociedade contemporânea. O enfoque parte principalmente de pequenos exemplos analisados em jornais e sites que têm sua importância reconhecida nos períodos analisados. <strong></strong></p>
Branded content e narcisismo: uma análise do case The Hire da BMW
Authors: De Quadros Ana Raquel Zimmermann , Schwartz Christian Luiz Melim 
Published: August 2016

<p class="Default"><span>A publicidade evoluiu com o passar do tempo, pois surgiu a necessidade de se criar novas estratégias para atingir um novo tipo de consumidor: o digital. Esse público já não pode ser exposto à publicidade comum, é preciso saber comunicar-se como esses novos indivíduos. Através do estudo de uma estratégia de marketing chamada <em>branded content</em>, este artigo irá analisar a narrativa utilizada no case <em>The Hire</em>, da BMW, e relacioná-la ao sucesso da campanha. Além disso, irá traçar uma ligação entre o consumidor digital e o narcisismo. </span></p>
A afirmação de estereótipos através dos discursos midiáticos: análise do caso Nicole Bahls e Gerald Thomas
Authors: Moura Keren Franciane , Nicaretta Giselle Marquette , Da Rosa Ana Paula 
Published: August 2016

<p>A imagem de determinadas mulheres dentro dos meios de comunicação é constantemente utilizada para difundir ideais de beleza e comportamento, afirmando e estabelecendo discursos sexistas que estereotipam a mulher como objeto de dominação masculina. Esse artigo propõe-se a examinar essa questão partindo do caso específico ocorrido entre a modelo Nicole Bahls e o diretor Gerald Thomas, através da análise do conteúdo noticiado por diferentes sites, visando identificar os estereótipos apresentados sobre uma mesma figura feminina nos diferentes discursos construídos pela mídia.</p><p> </p>
Comunicação integrada no terceiro setor: segmentação de público-alvo do Instituto Paz no Trânsito no Facebook
Authors: André Hendryo 
Published: August 2016

<p>O objetivo deste trabalho é avaliar o trabalho de três semestres de uma consultoria desenvolvida por estudantes de Jornalismo das Faculdades Integradas do Brasil (UniBrasil) para o Instituto Paz no Trânsito (IPTRAN). Esta organização não-governamental foi formada em 2010, um ano após a morte de dois jovens em um acidente de carro em Curitiba, que envolveu um ex-deputado e teve enorme repercussão na mídia em todo o país. Este estudo faz um diagnóstico com o objetivo de criar um plano de comunicação institucional para IPTRAN e mostra como essas ações foram implantadas. </p>
INTERTEXTUALIDADE E POLIFONIA TEOLÓGICA NO FILME FRANKENSTEIN: ENTRE ANJOS E DEMÔNIOS
Authors: Ramos Rangel Ramiro , Mandaji Carolina Fernandes da Silva 
Published: August 2016

<p><strong>ABSTRACT:</strong></p><p>This article aims to make a discursive analysis of theology and religious elements present in the film Frankenstein: Entre anjos e demônios. For this, two concepts are used in the Discourse Analysis: the Intertextuality and Polyphony. The objective of this research is to demonstrate the dialogism and the implicit and explicit heterogeneity between religious discourse and film production.</p><p><strong>RESUMO:</strong></p><p>O presente artigo se propõe a fazer uma análise discursiva da Teologia e dos elementos religiosos presentes no filme <em>Frankenstein: Entre anjos e demônios</em>. Para isso, serão utilizados dois conceitos da Análise do Discurso: a Intertextualidade e a Polifonia. O objetivo desta pesquisa é demonstrar o dialogismo e a heterogeneidade implícita e explícita entre o discurso religioso e a produção cinematográfica</p><p> </p>
A educação contemporânea através das imagens: juventude, cibercultura e formação docente.
Authors: Barros Patrícia Marcondes de 
Published: August 2016

<p>O presente artigo se constitui numa interface resultante da pesquisa de pós-doutorado em Educação que visa analisar quais são os desafios educacionais na formação de professores frente às novas gerações moduladas pela tecnologia, relacionadas à pós-modernidade. Trata-se de uma análise reflexiva acerca da imagem e suas reverberações no âmbito educacional na contemporaneidade. Os jovens “pós-modernos” ou “nativos digitais” formam o que se denominou Juventude “Y” (geração do milênio ou geração digital) e “Z”, oriundos da sociedade imagética e de grande desenvolvimento tecnológico. Uma das questões que se impõe à educação para as gerações atuais é a do entendimento dessa cultura permeada de imagens que, como agentes simbólicos, nos fornecem subsídios para o entendimento dos novos valores contemporâneos, propiciando a partir disso, a ressignificação das práticas pedagógicas, indo além das fronteiras da educação formal.</p>
Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages
Authors: Howes Ashleigh , Taubert Christina , Blankley Simon , Spink Natasha , Wu Xuemei , Graham Christine M. , Zhao Jiawen , Saraiva Margarida , Ricciardi-Castagnoli Paola , Bancroft Gregory J. , O’Garra Anne 
Published: August 2016

Abstract
Pattern recognition receptors detect microbial products and induce cytokines, which shape the immunological response. IL-12, TNF-α, and IL-1β are proinflammatory cytokines, which are essential for resistance against infection, but when produced at high levels they may contribute to immunopathology. In contrast, IL-10 is an immunosuppressive cytokine, which dampens proinflammatory responses, but it can also lead to defective pathogen clearance. The regulation of these cytokines is therefore central to the generation of an effective but balanced immune response. In this study, we show that macrophages derived from C57BL/6 mice produce low levels of IL-12, TNF-α, and IL-1β, but high levels of IL-10, in response to TLR4 and TLR2 ligands LPS and Pam3CSK4, as well as Burkholderia pseudomallei, a Gram-negative bacterium that activates TLR2/4. In contrast, macrophages derived from BALB/c mice show a reciprocal pattern of cytokine production. Differential production of IL-10 in B. pseudomallei and LPS-stimulated C57BL/6 and BALB/c macrophages was due to a type I IFN and ERK1/2-dependent, but IL-27–independent, mechanism. Enhanced type I IFN expression in LPS-stimulated C57BL/6 macrophages was accompanied by increased STAT1 and IFN regulatory factor 3 activation. Furthermore, type I IFN contributed to differential IL-1β and IL-12 production in B. pseudomallei and LPS-stimulated C57BL/6 and BALB/c macrophages via both IL-10–dependent and –independent mechanisms. These findings highlight key pathways responsible for the regulation of pro- and anti-inflammatory cytokines in macrophages and reveal how they may differ according to the genetic background of the host.
The Lymphatic Immune Response Induced by the Adjuvant AS01: A Comparison of Intramuscular and Subcutaneous Immunization Routes
Authors: Neeland Melanie R. , Shi Wei , Collignon Catherine , Taubenheim Nadine , Meeusen Els N. T. , Didierlaurent Arnaud M. , de Veer Michael J. 
Published: August 2016

Abstract
The liposome-based adjuvant AS01 incorporates two immune stimulants, 3-O-desacyl-4′-monophosphoryl lipid A and the saponin QS-21. AS01 is under investigation for use in several vaccines in clinical development. i.m. injection of AS01 enhances immune cell activation and dendritic cell (DC) Ag presentation in the local muscle-draining lymph node. However, cellular and Ag trafficking in the lymphatic vessels that connect an i.m. injection site with the local lymph node has not been investigated. The objectives of this study were: 1) to quantify the in vivo cellular immune response induced by AS01 in an outbred ovine model, 2) to develop a lymphatic cannulation model that directly collects lymphatic fluid draining the muscle, and 3) to investigate the function of immune cells entering and exiting the lymphatic compartments after s.c. or i.m. vaccination with AS01 administered with hepatitis B surface Ag (HBsAg). We show that HBsAg-AS01 induces a distinct immunogenic cellular signature within the blood and draining lymphatics following both immunization routes. We reveal that MHCIIhigh migratory DCs, neutrophils, and monocytes can acquire Ag within muscle and s.c. afferent lymph, and that HBsAg-AS01 uniquely induces the selective migration of Ag-positive neutrophils, monocytes, and an MHCIIhigh DC-like cell type out of the lymph node via the efferent lymphatics that may enhance Ag-specific immunity. We report the characterization of the immune response in the lymphatic network after i.m. and s.c. injection of a clinically relevant vaccine, all in real time using a dose and volume comparable with that administered in humans.
ERβ-Dependent Direct Suppression of Human and Murine Th17 Cells and Treatment of Established Central Nervous System Autoimmunity by a Neurosteroid
Authors: Aggelakopoulou Maria , Kourepini Evangelia , Paschalidis Nikolaos , Simoes Davina C. M. , Kalavrizioti Dimitra , Dimisianos Nikolaos , Papathanasopoulos Panagiotis , Mouzaki Athanasia , Panoutsakopoulou Vily 
Published: August 2016

Abstract
Multiple sclerosis (MS), an autoimmune disease of the CNS, is mediated by autoreactive Th cells. A previous study showed that the neurosteroid dehydroepiandrosterone (DHEA), when administered preclinically, could suppress progression of relapsing-remitting experimental autoimmune encephalomyelitis (EAE). However, the effects of DHEA on human or murine pathogenic immune cells, such as Th17, were unknown. In addition, effects of this neurosteroid on symptomatic disease, as well as the receptors involved, had not been investigated. In this study, we show that DHEA suppressed peripheral responses from patients with MS and reversed established paralysis and CNS inflammation in four different EAE models, including the 2D2 TCR-transgenic mouse model. DHEA directly inhibited human and murine Th17 cells, inducing IL-10–producing regulatory T cells. Administration of DHEA in symptomatic mice induced regulatory CD4+ T cells that were suppressive in an IL-10–dependent manner. Expression of the estrogen receptor β by CD4+ T cells was necessary for DHEA-mediated EAE amelioration, as well as for direct downregulation of Th17 responses. TGF-β1 as well as aryl hydrocarbon receptor activation was necessary for the expansion of IL-10–producing T cells by DHEA. Thus, our studies demonstrate that compounds that inhibit pathogenic Th17 responses and expand functional regulatory cells could serve as therapeutic agents for autoimmune diseases, such as MS.
Revisiting a family approach in youth ministry
Authors: Cloete Anita 
Published: August 2016

The primary aim of this article is to reflect on the ‘family’ of a family approach in youth ministry. The overall aim of the article is to confirm the importance of family as social and, specifically, a religious institution. Therefore, a family approach in youth ministry is of utmost importance, but it is argued that it is more feasible if the responsibility is taken up to continuously reflect on being a family in contemporary society. That implies that reflection on family is as much a cultural task as a theological task. Therefore, an interdisciplinary reflection is valued as cultural, is multifaceted and an ever chancing phenomenon. One of the outstanding characteristic of families today is diversity. Diversity, with regards to family, is mostly related to the structure of family and attention has therefore been paid to it in a section of this article. It is argued that diversity should not be perceived as negative, because, despite of the diverse structure of family, constructive relationships is proposed as the binding and constitutive factor in being a family. The focus on relatedness moves beyond blood ties to relatedness and solidarity as fundamental for our humanness. Thatcher (2007:6) differentiates between a structural and relational approach to family. A relational approach is opted here with the emphasis on the quality of the dimension of the relationships. These relationships are also understood to be grounded in theological sources such as the Person of God and the church.