bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mitochondrial Rho-GTPase, Miro, is resident at peroxisomes and regulates peroxisomal trafficking and morphology
Authors: Covill-Cooke Christian , López-Doménech Guillermo , Birsa Nicol , Kittler Josef T. 
Published: December 2017

AbstractPeroxisomes are essential for a number of cellular functions, including reactive oxygen species metabolism, fatty acid β-oxidation and lipid biosynthesis. To ensure optimal functionality of peroxisome-dependent processes throughout the cell they must be trafficked; however, peroxisomal transport remains poorly characterised. Here we show that Miro1 and Miro2, outer mitochondrial membrane proteins essential for mitochondrial trafficking, are also localised to peroxisomes. Peroxisomal localisation of Miro1 is negatively regulated by its first GTPase domain and is mediated by an interaction through its transmembrane domain with the peroxisomal-membrane protein chaperone, Pex19. By using Miro1/2 double knockout mouse embryonic fibroblasts (MEFs) we find that the loss of Miro1/2 leads to a significant reduction in short-range microtubule-independent peroxisomal motility. Additionally, Miro regulates peroxisomal size and morphology. Our results contribute to the fundamental understanding of peroxisomal trafficking and morphology, supporting a complex crosstalk between peroxisomal and mitochondrial biology.
Factor analysis on the Tasmanian topsoil microscopic community
Authors: Kakar Ayem , Kolezeva Yelena 
Published: December 2017

AbstractTo help with stand restoration, the influence of width size on the Tasmanian topsoil microscopic community was studied in anAthrotaxis cupressoidesstand suffering from hail storm damage. The functional diversity of topsoil microbial groups was estimated from degradation of 31 substrates on Bencho EcoPlates. Using Factor Analysis (FA) we found width size had a significant influence on average column colorimetric disseminator analysis (AVGCLR) and on the Rao indices of topsoil microbial diversity. Compared with large widths, small widths had higher AVGCLR. The ten widths were divided into three groups by cluster analysis and FA: group 1 reflected large widths, while groups 2 and 3 reflected small widths. Thirty-one sole carbon sources were divided into three groups by FA. Using an eigenvector greater than 0.5 as a standard for checking carbon (C) sources, nineteen kinds of C sources included in principal components 1 and 2 had a relatively high influence on the topsoil microbial community, including carbohydrates, amino acids and carboxylic acids. This indicates that the use by topsoil microorganisms of carboxylic acids, sugars and amino acids was greater than other C sources. These findings suggest that width size played a key role in the topsoil microbial diversity after a natural disturbance.
Genome-wide association acrossSaccharomyces cerevisiaestrains reveals substantial variation in underlying gene requirements for toxin tolerance
Authors: Sardi Maria , Paithane Vaishnavi , Place Michael , Robinson De Elegant , Hose James , Wohlbach Dana J. , Gasch Audrey P. 
Published: December 2017

AbstractCellulosic plant biomass is a promising sustainable resource for generating alternative biofuels and biochemicals with microbial factories. But a remaining bottleneck is engineering microbes that are tolerant of toxins generated during biomass processing, because mechanisms of toxin defense are only beginning to emerge. Here, we exploited natural diversity in 165Saccharomyces cerevisiaestrains isolated from diverse geographical and ecological niches, to identify mechanisms of hydrolysate-toxin tolerance. We performed genome-wide association (GWA) analysis to identify genetic variants underlying toxin tolerance, and gene knockouts and allele-swap experiments to validate the involvement of implicated genes. In the process of this work, we uncovered a surprising difference in genetic architecture depending on strain background: in all but one case, knockout of implicated genes had a significant effect on toxin tolerance in one strain, but no significant effect in another strain. In fact, whether or not the gene was involved in tolerance in each strain background had a bigger contribution to strain-specific variation than allelic differences. Our results suggest a major difference in the underlying network of causal genes in different strains, suggesting that mechanisms of hydrolysate tolerance are very dependent on the genetic background. These results could have significant implications for interpreting GWA results and raise important considerations for engineering strategies for industrial strain improvement.Author summaryUnderstanding the genetic architecture of complex traits is important for elucidating the genotype-phenotype relationship. Many studies have sought genetic variants that underlie phenotypic variation across individuals, both to implicate causal variants and to inform on architecture. Here we used genome-wide association analysis to identify genes and processes involved in tolerance of toxins found in plant-biomass hydrolysate, an important substrate for sustainable biofuel production. We found substantial variation in whether or not individual genes were important for tolerance across genetic backgrounds. Whether or not a gene was important in a given strain background explained more variation than the alleleic differences in the gene. These results suggest substantial variation in gene contributions, and perhaps underlying mechanisms, of toxin tolerance.
A Regression-based Framework for Scalable Pathway-guided Search in Genome-wide Association Studies
Authors: Biswas Shrayashi , Pal Soumen , Bhattacharjee Samsiddhi 
Published: December 2017

AbstractTraditional unbiased genome-wide association studies (GWAS) have successfully identified thousands of loci associated with various complex diseases but there is evidence to suggest that many variants were missed at stringent genome-wide thresholds. Fortunately, there is a rapidly increasing amount of prior knowledge in publicly available genomic datasets and biological databases that can be harnessed to enhance the power of discovering SNPs/Genes from existing or new GWAS datasets. For most diseases, many of the identified loci tend to cluster into a few specific biological pathways/networks. From the point of view of disease etiology, such clustering is generally to be expected. This phenomenon can be exploited to conduct a more powerful genome-wide scan that is tailored to identify loci that are interconnected in pathways. We propose a scalable regression-based analytical framework to enable such a pathway-guided GWAS and demonstrate that it provides significant gains in power to detect disease associated SNPs. Our method requires two inputs, namely a) genome-wide summary level data (e.g., SNP p-values) and b) a grouping of genes into biologically meaningful categories (e.g., a database of pathways). It automatically adjusts the input p-values by incorporating the knowledge derived adaptively from the data and the pathways specified. The method involves a regularized logistic regression analysis to derive priors of each SNP and then re-weights the p-values of SNPs so as to maximize overall power of making discoveries. It increases the power to discover SNPs co-clustering into some of these pathways, while maintaining the global type-1 error (FWER) at the desired level. We used whole-genome simulations and summary data from real GWA studies of psoriasis, SLE, coronary artery disease and type-2 diabetes to illustrate the power improvement achieved by pathway-guided search. Our pipeline implemented as an R package can flexibly handle large number of prior annotations possibly derived from multiple databases.
Myopic control of neural dynamics
Authors: Hocker David , Park Il Memming 
Published: December 2017

AbstractManipulating the dynamics of neural systems through targeted stimulation is a frontier of research and clinical neuroscience; however, the control schemes considered for neural systems are mismatched for the unique needs of manipulating neural dynamics. An appropriate control method should respect the variability in neural systems, incorporating moment to moment “input” to the neural dynamics and behaving based on the current neural state, irrespective of the past trajectory. We propose such a controller under a nonlinear state-space feedback framework that steers one dynamical system to function as through it were another dynamical system entirely. This “myopic” controller is formulated through a novel variant of a model reference control cost that manipulates dynamics in a short-sighted manner that only sets a target trajectory of a single time step into the future (hence its myopic nature), which omits the need to pre-calculate a rigid and computationally costly neural feedback control solution. To demonstrate the breadth of this control’s utility, two examples with distinctly different applications in neuroscience are studied. First, we show the myopic control’s utility to probe the causal link between dynamics and behavior for cognitive processes by transforming a winner-take-all decision-making system to operate as a robust neural integrator of evidence. Second, an unhealthy motor-like system containing an unwanted beta-oscillation spiral attractor is controlled to function as a healthy motor system, a relevant clinical example for neurological disorders.
The Class I E3 Ubiquitin Ligase TRIM67 Modulates Brain Development and Behavior
Authors: Boyer Nicholas P. , Monkiewicz Caroline , Moy Sheryl S. , Gupton Stephanie L. 
Published: December 2017

ABSTRACTSpecific class I members of the TRIM family of E3 ubiquitin ligases have been implicated in neuronal development from invertebrates to mammals. The single invertebrate class I TRIM and mammalian TRIM9 regulate axon branching and guidance in response to the axon guidance cue netrin-1, whereas mammalian TRIM46 establishes the axon initial segment. In humans, mutations in TRIM1 and TRIM18 are implicated in Optiz Syndrome, characterized by midline defects and often mild intellectual disability. TRIM67 is the most evolutionarily conserved vertebrate class I TRIM, yet is the least studied. Here we show that TRIM67 interacts with both its closest paralog TRIM9 and the netrin receptor DCC, and is differentially enriched in specific brain regions at specific developmental points. We describe the anatomical and behavioral consequences of deletion of murine Trim67. While viable, mice lacking Trim67 display severe impairments in spatial memory, cognitive flexibility, social novelty preference, muscle function and sensorimotor gating. Additionally, they exhibit abnormal anatomy of several brain regions, including the hippocampus, striatum and thalamus, as well as the corpus callosum. This study demonstrates the necessity for TRIM67 in appropriate brain development and function.SIGNIFICANCE STATEMENTAs a family, class I TRIM E3 ubiquitin ligases play important roles in neuronal development and function, potentially cooperatively. TRIM67 is the most evolutionarily conserved class I TRIM and is developmentally regulated and brain-enriched. Deletion of murine Trim67 results in malformations of a subset subcortical brain regions and of cortical and subcortical myelinated fiber tracts, as well as deficits in spatial memory, motor function, sociability and sensorimotor gating. We conclude that TRIM67 is critical for appropriate brain development and behavior, potentially downstream of the axon guidance cue netrin, and in cooperation with class I TRIM9.
Narratives of recovery in people with coexisting mental health and alcohol misuse difficulties
Authors: Stott Andrew , Priest Helena 
Published: December 2017

Purpose
Existing literature has examined what recovery means to people with co-occurring difficulties, but does little to examine experiences of recovery as a process. The purpose of this paper is to use a narrative approach to explore the process of recovery as an individual journey in a social context. It focuses on people who use alcohol in order to explore the impact of alcohol’s specific cultural meanings on the recovery journey.

Design/methodology/approach
Ten interviews with people with coexisting mental health and alcohol misuse difficulties were conducted, audio-recorded, and transcribed. The transcriptions were analysed using narrative analysis.

Findings
Most participants’ narratives shared a three-part structure, from a traumatic past, through an episode of change, to an ongoing recovery phase. Change and recovery were attributed to several factors including flexible and practical support from services, therapeutic relationships with key professionals, and peer support. Some participants redefined themselves and their alcohol use in relation to ideas of what it is to be “normal”.

Research limitations/implications
The research excluded people who recover outside of services, replicating a shortcoming of much research in this area.

Practical implications
The value placed on professionals having specialised therapeutic skills in working with trauma highlights the need for training in this area. The role for practical and material support underlines the importance of multi-agency working.

Originality/value
The narrative methodology enables the study to draw links between personal stories of recovery and wider social influences, allowing comment on the implications for services. Further, the experiences of people with coexisting mental health and alcohol misuse difficulties have rarely been studied apart from the dual diagnosis population in general, so this paper is able to investigate the specific challenges for this population.
An investigation of cross-cultural social tagging behaviours between Chinese and Americans
Authors: Hu Qian , Lin Xin , Han Shuguang , Li Lei 
Published: December 2017

Purpose
The purpose of this paper is to explore different tagging behaviours between Chinese and Americans by analysing the movie tags, and explore the feasibility of applying cultural differences to tag recommendations.

Design/methodology/approach
This paper introduced hypotheses based on several well-established psychological theories and tested them with social tags for the same movies generated by Chinese and Americans. And to prove the utility of the cultural factor consideration, this paper conducted a cross-cultural tag recommendation experiment.

Findings
The results show that compared with Americans, Chinese users tend to add more tags about movies’ background information (e.g. release year) and global contextual characteristics (e.g. genre); they also prefer to add tags about production countries and factual tags, and cultural factors can be applied for recommending more accurate tags.

Research limitations/implications
Other reasons for tagging differences beyond cultural factors have not be explored. Tags for some sample movies in MovieLens might be unstable, as they had been tagged by a small scale of users; as a result, the tags’ type distribution might be influenced.

Practical implications
The results and conclusion of this study will be beneficial for the cross-cultural applications of social tags and mining users’ interests based on tags.

Originality/value
This paper provided a deeper investigation of the cross-cultural effect in people’s social tagging behaviours from cognitive perspective, and an empirical analysis has been performed to explore proper approaches of incorporating cultural differences for tag recommendation.
Experiences of practice-based learning in phenomenographic perspective
Authors: Rovio-Johansson Airi 
Published: December 2017

PurposeThe paper aims to examine, within the context of professional practice and learning, how designers collaboratively working in international teams experience practice-based learning and how such occasions contribute to professional development.Design/methodology/approachThe paper introduces the cooperation project between Tibro Training Centre and Furniture Technology Centre Trust and its workshop context organized as practice-based learning. Participants’ learning context consisted of a mixture of professional practices allowing different logics and different cultures make up an innovative working site. Qualitative analysis of semi-structured interview data suggests that three phenomenographic hierarchical categories constitute the learning process: getting a recognized professional identity; perceiving new elements and expanding knowledge and seeing new aspects of design work and new steps of development in profession.FindingsCooperative practice-based learning is understood as social practice in a community of practice, and as continuous changes of the learning object due to that new aspects are discerned by the learners. These categories illustrate how participants’ meaning making and understanding of the learning object were expressed in cooperation as doings and sayings, as translation and as situated activities in a community of practice. Accordingly, it contributed to participants’ professional development in spite of their different professional educations and professional experiences.Practical implicationsMore studies of practice-based learning environments in work places are needed that could help societies and companies to advance integrative efforts of new employees and new immigrants into an increasingly diverse globalized labour market.Originality/valueThe results suggest that understanding as well as content structure and meaning making of the learning object are intertwined constituent aspects of practice-based learning.
Instrumental work values and responses to HR practices
Authors: Huo Meng-Long , Boxall Peter 
Published: December 2017

PurposeGrounded in the theory of person-organisation fit, the purpose of this paper is to investigate the extent to which instrumental work values influence the relationship between HR practices and employee well-being (measured by job satisfaction) in a sample of Chinese workers.Design/methodology/approachQuestionnaire data for this cross-sectional, quantitative study were collected from 371 front-line workers in a Chinese manufacturer. Structural equation modelling was used to test the hypotheses.FindingsThe results show that work instrumentalism significantly reduces the positive effect of training on job satisfaction while boosting the positive effect of remuneration on job satisfaction. In contrast, there is no evidence for an interaction between instrumentalism and employee involvement.Practical implicationsThe results imply that the degree to which HR practices are effective in promoting job satisfaction among these Chinese workers depends both on their work-value orientations and on the implications of the particular HR practice. Managers concerned about job satisfaction in China need to consider the impact of work values and the goals of particular HR practices.Originality/valueChina makes an enormously important contribution to world manufacturing output but the authors need a better understanding of how Chinese workers are likely to interpret and respond to HR practices if employee well-being in Chinese enterprises is to be fostered.
Workplace bullying and employee silence
Authors: Rai Arpana , Agarwal Upasna A. 
Published: December 2017

Purpose
The purpose of this paper is to examine the effects of workplace bullying on employee silence (defensive, relational, and ineffectual silence), and to test the mediating role of psychological contract violation (PCV) in this relationship and the extent to which the mediation is moderated by workplace friendship.

Design/methodology/approach
Data were collected from 835 full-time Indian managerial employees working in different Indian organizations.

Findings
Results revealed that workplace bullying positively correlated with silence (defensive, relational, and ineffectual silence). The hypothesized moderated mediation condition was supported as results suggest that PCV mediated the bullying-silence relationship and workplace friendship moderated this mediating pathway, i.e. indirect effects of workplace bullying on employee silence via PCV were weaker for employees with high workplace friendship.

Research limitations/implications
A cross-sectional design, use of self-reported questionnaires, and gender-blind perspective to examine bullying are few limitations of this study.

Practical implications
This is the first study examining employee silence in response to workplace bullying and one of the few attempts to examine employees’ passive coping strategies in response to workplace mistreatment. This study is also one of the rare attempts to examine bullying-outcomes relationship in the Indian context.

Social implications
A well-formulated and effectively implemented anti-bullying policy and management support may encourage employees to combat bullying by raising their voices against it.

Originality/value
This is the first study examining employee silence in response to workplace bullying. This study is also one of the rare attempts to examine bullying-outcomes relationship in the Indian context.
Abusive supervision and knowledge sharing: the moderating role of organizational tenure
Authors: Kim Seckyoung Loretta , Son Seung Yeon , Yun Seokhwa 
Published: December 2017

PurposeDrawing on social exchange theory (SET) and conservation of resources (COR) theory, the purpose of this paper is to examine the negative consequence of abusive supervision on knowledge sharing. Further, this paper explores the moderating role of organizational tenure in the relationship between abusive supervision and employees’ knowledge sharing behavior applying sense-making theory.Design/methodology/approachThe hypotheses were tested using regression analysis across two independent studies conducted in South Korea.FindingsThe authors found evidence that there was a negative relationship between abusive supervision and employees’ knowledge sharing behavior. Furthermore, the aforementioned relationship was strengthened for those with longer organizational tenure.Originality/valueThis research deepens our understanding of the negative consequences of abusive supervision on employees’ knowledge sharing behavior by drawing on SET and COR theory. Through two independent studies, the results demonstrated that employees who receive abusive treatment from their supervisors tend to reduce their knowledge sharing behavior. Furthermore, this detrimental effect is stronger for key knowledge providers, namely long-tenured employees.
Creating a high performing organization in today’s data driven world
Authors: Pozzo Heidi 
Published: December 2017

Purpose
The purpose of this paper is to outline what high-performing organizations do to achieve success. In today’s data-driven world, it is easy to get lost in the weeds and not achieve the results you desire. High-performing organizations have figured out how to create focus and leverage resources to achieve outsized results. The paper delves into organizations undergoing transformations and highlights what sets apart those that were successful from those that were not.

Design/methodology/approach
This paper is designed as a viewpoint paper that draws on observations made by the author. Case studies are used to demonstrate the findings and the actions taken that were either successful or not.

Findings
High-performing organizations create focus and leverage resources. The most successful examples have undergone a holistic transformation that was supported by technology.

Originality/value
This paper will provide a roadmap to leaders who want to transform their business into a high-performing organization.