bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research progress on plant stress‐associated protein (SAP) family: Master regulators to deal with environmental stresses
Authors: Ben Saad Rania , Ben Romdhane Walid , Čmiková Natália , Baazaoui Narjes , Bouteraa Mohamed Taieb , Ben Akacha Bouthaina , Chouaibi Yosra , Maisto Maria , Ben Hsouna Anis , Garzoli Stefania , Wiszniewska Alina , Kačániová Miroslava 
Published: September 2024

AbstractEvery year, unfavorable environmental factors significantly affect crop productivity and threaten food security. Plants are sessile; they cannot move to escape unfavorable environmental conditions, and therefore, they activate a variety of defense pathways. Among them are processes regulated by stress‐associated proteins (SAPs). SAPs have a specific zinc finger domain (A20) at the N‐terminus and either AN1 or C2H2 at the C‐terminus. SAP proteins are involved in many biological processes and in response to various abiotic or biotic constraints. Most SAPs play a role in conferring transgenic stress resistance and are stress‐inducible. The emerging field of SAPs in abiotic or biotic stress response regulation has attracted the attention of researchers. Although SAPs interact with various proteins to perform their functions, the exact mechanisms of these interactions remain incompletely understood. This review aims to provide a comprehensive understanding of SAPs, covering their diversity, structure, expression, and subcellular localization. SAPs play a pivotal role in enabling crosstalk between abiotic and biotic stress signaling pathways, making them essential for developing stress‐tolerant crops without yield penalties. Collectively, understanding the complex regulation of SAPs in stress responses can contribute to enhancing tolerance against various environmental stresses through several techniques such as transgenesis, classical breeding, or gene editing.
Beyond RNAi: How the Dicer protein modulates the antiviral innate immune response in mammalian cells
Authors: Gaucherand Léa , Baldaccini Morgane , Pfeffer Sébastien 
Published: September 2024

AbstractWhile Dicer plays an important antiviral role through the RNAi pathway in plants and invertebrates, its contribution to antiviral immunity in vertebrates and more specifically mammals is more controversial. The apparent limited RNAi activity in mammalian cells has been attributed to the reduced long dsRNA processive activity of mammalian Dicer, as well as a functional incompatibility between the RNAi and IFN pathways. Why Dicer has lost this antiviral activity in the profit of the IFN pathway is still unclear. We propose that the primary direct antiviral activity of Dicer has been functionally replaced by other sensors in the IFN pathway, leading to its specialization toward microRNA maturation. As a result, Dicer can regulate the innate immune response and prevent basal activation of the IFN pathway in mammals. Here, we discuss this hypothesis, highlighting how the adaptation of the helicase domain of mammalian Dicer may be key to this process.
Cas‐CLOVER‐mediated knockout of STAT1: A novel approach to engineer packaging HEK‐293 cell lines used for rAAV production
Authors: Andorfer Peter , Kahlig Carolin‐Isabel , Pakusic Doris , Pachlinger Robert , John Christiane , Schrenk Irene , Eisenhut Peter , Lengler Johannes , Innthaler Bernd , Micutkova Lucia , Kraus Barbara , Brizzee Corey , Crawford Jack , Hernandez Bort Juan A. 
Published: September 2024

AbstractIn addressing the limitations of CRISPR‐Cas9, including off‐target effects and high licensing fees for commercial use, Cas‐CLOVER, a dimeric gene editing tool activated by two guide RNAs, was recently developed. This study focused on implementing and evaluating Cas‐CLOVER in HEK‐293 cells used for recombinant adeno‐associated virus (rAAV) production by targeting the signal transducer and activator of transcription 1 (STAT1) locus, which is crucial for cell growth regulation and might influence rAAV production yields. Cas‐CLOVER demonstrated impressive efficiency in gene editing, achieving over 90% knockout (KO) success. Thirteen selected HEK‐293 STAT1 KO sub‐clones were subjected to extensive analytical characterization to assess their genomic stability, crucial for maintaining cell integrity and functionality. Additionally, rAAV9 productivity, Rep protein pattern profile, and potency, among others, were assessed. Clones showed significant variation in capsid and vector genome titers, with capsid titer reductions ranging from 15% to 98% and vector genome titers from 16% to 55%. Interestingly, the Cas‐CLOVER‐mediated STAT1 KO bulk cell population showed a better ratio of full to empty capsids. Our study also established a comprehensive analytical workflow to detect and evaluate the gene KOs generated by this innovative tool, providing a solid groundwork for future research in precise gene editing technologies.
Is national economic growth associated with environmental social and governance performance of firms?
Authors: Biju Ajithakumari Vijayappan Nair , Jayachandran Ambili , Thomas Ann Susan , Sasidharan Aghila 
Published: September 2024

AbstractThis study delves into the effect of environmental, social and governance (ESG) indicators on economic growth trajectory of an emerging economy. A sample of firms listed on the ESG index of the National Stock Exchange (NSE) was examined. The findings reveal a positive and statistically significant relationship between ESG performance indicators and economic growth represented as gross domestic product (GDP) per capita. The sample is further segregated into energy and non‐energy sectors, where the aggregate ESG scores exhibited a statistically significant influence on economic growth in both sectors. However, upon a closer examination of the individual ESG pillars, the governance pillar is observed to exert a negative influence on economic performance in non‐energy firms. In contrast, the environment and social pillars show no significant influence. The analysis then employs a unique approach by applying a Difference‐in‐differences estimator to assess the impact of Business Responsibility and Sustainability Reporting (BRSR) regulations, which confirms a positive impact on economic growth after regulations but exclusively within the non‐energy sector. This study underscores the substantial impact of ESG aggregate and individual scores on GDP per capita, which translates to economic growth.
Transcatheter aortic valve implantation for severe aortic regurgitation using the J‐Valve system: A midterm follow‐up study
Authors: Jin Min , Zhang Haitao , Zhou Qing , Li Shuchun , Wang Dongjin 
Published: September 2024

AbstractBackgroundTranscatheter aortic valve implantation (TAVI) is a well‐established intervention for severe aortic valve stenosis. However, its application for severe aortic regurgitation (AR) is still under evaluation. This study aims to present the 3‐year follow‐up outcomes of the J‐Valve system in managing severe AR.AimsThe aim of this study was to evaluate the mid‐term efficacy and durability of the J‐Valve system in the treatment of severe AR and to provide new information on this intervention.MethodsIn this retrospective, single‐center study, we evaluated the prognostic outcomes of patients with AR, who underwent treatment with the J‐Valve system at Nanjing Drum Tower Hospital. Consecutive patients who were treated with the J‐Valve were included in the analysis. The study focused on the echocardiographic follow‐up to assess the effectiveness and durability of the J‐Valve system in managing AR.ResultsFrom January 2018 to December 2022, 36 high‐risk AR patients treated with the J‐Valve system had a procedural success rate of 97.2%, with one case requiring open‐heart surgery due to valve displacement. Significant improvements were observed in left ventricular diameter (from 63.50 [58.75–69.50] mm to 56.50 [53.00–60.50] mm, p < 0.001) and left atrial diameter (from 44.00 [40.00–45.25] mm to 39.00 [36.75–41.00] mm, p = 0.003) postsurgery. All patients completed the 1‐year follow‐up, with an overall mortality rate of 2 out of 36 (5.6%). Among the surviving patients, there was one case of III° atrioventricular block and one case of stroke, both occurring within 90 days postsurgery. After a 3‐year follow‐up, 15.0% of patients had mild or moderate valvular regurgitation, with no cases of moderate or severe paravalvular leak. Additionally, 89.5% of patients were classified as New York Heart Association class I or II, showing significantly enhanced cardiac function.ConclusionThe J‐Valve system has shown positive therapeutic outcomes in treating AR, with notable effectiveness in managing the condition and significant improvements in heart failure symptoms and cardiac remodeling. However, due to the limited sample size and partial follow‐up data, it is important to emphasize the need for further research with comprehensive long‐term follow‐up, to fully validate these results.
Effects of Acid Sites on Thin MCM‐22 Zeolite Catalysts and Their Catalytic Applications for Acetylene Aromatization
Authors: Hong Jinju , Jeon Jonghyun , Kim Hee Hwan , Shin Kyung Soo , Cho Sung June , Ha Kyoung‐Su 
Published: September 2024

AbstractTwo types of thin zeolite MCM‐22 catalysts were prepared by using a carbon sphere template. By applying different calcination methods, a hollow sphere‐type MCM‐22 catalyst (HS‐MCM‐22) and a nanosheet‐type MCM‐22 catalyst (NS‐MCM‐22) were synthesized. Those catalysts were tested and evaluated for acetylene aromatization to see the effects of thin structures. The two types of thin catalysts were found to have higher amounts of acid sites than those of the conventional MCM‐22 catalyst. It was found that the extremely short diffusion length not only enhanced the aromatic yield, but also suppressed the formation of graphitic coke. Notably, the diffusion length of NS‐MCM‐22 was found to be at least 15 times shorter than that of conventional MCM‐22, leading to an 11% and 18% increase in benzene yield, respectively. The thin structure seemed to help the produced aromatics efficiently desorb before they were further converted into carbon precursors and coke. According to the thermogravimetric analysis, the carbon species in the spent thin catalysts were found less graphitic than that of the conventional MCM‐22 catalyst. Because of this, the thin MCM‐22 catalysts were believed to show higher coke removal capability. Especially, the coke removal rate of NS‐MCM‐22 was estimated over 90% despite the severe carbon deposition during the reaction.
Exploring Dinuclear Titanium Complexes in Titanium(III) Catalysis
Authors: Unkrig‐Bau Michael A. , Leijendekker Sara L. , Streuff Jan 
Published: September 2024

AbstractThe synthesis of one achiral and two chiral propylene‐bridged dititanocenes and their evaluation as catalysts in titanium(III) catalyzed ketone‐nitrile coupling reactions are reported. A reaction progress kinetics analysis of the cross‐coupling between acetophenone and benzyl cyanide reveals that, using a dinuclear titanocene catalyst, the order in catalyst is reduced from two to one in comparison to a mononuclear catalyst. Although the obtained coupling yields and enantioselectivities did not reach the results obtained with the latter, these examples constitute a proof of concept for the templated C─C coupling through coordination of ketone and nitrile to the two tethered titanium centers of a dinuclear catalyst.
Heteroatom‐Containing Zeolites as Solid Lewis Acid Catalysts for the Cycloaddition of CO2 to Epoxides
Authors: Gao Bohai , Li Weijie , Chai Yuchao , Wu Guangjun , Li Landong 
Published: September 2024

AbstractThe catalytic cycloaddition of CO2 to epoxides to produce valuable cyclic carbonates represents a simple and promising strategy for CO2 utilization, circumventing the ineffective CO2 reduction process. Despite current progresses, there remains an impending demand for highly‐active, cost‐effective and stable catalysts especially the ideal heterogeneous catalytic systems. Herein, we report the preparation of heteroatom‐containing zeolites through a two‐step process comprising of framework dealumination and subsequent heteroatom incorporation, and their catalytic applications in CO2 cycloaddition to epoxides. Characterization results reveal the successful incorporation of heteroatoms into framework to derive Lewis acidic M‐Beta zeolites (M = Ti, Zr or Hf). The as‐prepared M‐Beta Lewis acids show remarkable performance in the model reaction of CO2 cycloaddition to propylene oxide with the assistance of potassium iodide under solvent‐free conditions. The reaction parameters have been optimized employing Ti‐Beta catalyst and the substrate scope has been investigated. Finally, the impact of Lewis acidity on the cycloaddition reaction is discussed and the actual bifunctional Ti‐Beta/KI catalyst system is proposed, which is of important significance for the understanding of CO2 catalytic cycloaddition to epoxides.
Computationally optimized multi‐port antenna systems for WLAN/Wi‐Fi (IEEE 802.11a/h/j/n/ac/ax), 5G (mid‐band), and UWB applications
Authors: Gajbhiye Pradnya A. , Singh Satya P. , Sharma Madan Kumar 
Published: September 2024

SummaryThis paper presents computationally optimized 2‐element and 4‐element Multiple‐Input, Multiple‐Output (MIMO) antennas for WLAN/Wi‐Fi, 5G, and UWB applications. The antenna configuration is constructed with the orthogonal placement of sawtooth‐shaped circular monopole radiating elements. The Particle Swarm Optimization (PSO) and Covariance Matrix Adaptation Evolution Strategy (CMA‐ES) optimization techniques are employed to achieve the best performance and size of the proposed antenna. Among these optimization techniques, CMA‐ES is identified as the better approach. The final optimized geometry of the 2‐element and 4‐element MIMO antennas is 49.40 mm × 24.22 mm and 49.44 mm × 45 mm, respectively. Both optimized antennas are fabricated and experimentally verified. The fractional bandwidth of the antenna is more than 110.3%, and more than −20 dB isolation is attained without employing any decoupling method. The Envelop Correlation Coefficient (ECC), Directivity Gain (DG), Total Active Reflection Coefficient (TARC), and Channel Capacity Limit (CCL) are 0.0001, 9.99, < −15 dB, and 0.1 bits/s/Hz, respectively. The proposed antenna is a good candidate for numerous current wireless applications due to its size and performance.
A new perspective on antiangiogenic antibody drug resistance: Biomarkers, mechanisms, and strategies in malignancies
Authors: Zhao Chen , Zeng Yuan , Kang Nannan , Liu Yu 
Published: September 2024

AbstractDrug resistance of malignant tumor leads to disease progression be the bottleneck in clinical treatment. Antiangiogenic therapy, which aims to “starve” the tumor by inhibiting angiogenesis, is one of the key strategies in clinical oncology treatments. Recently, dozens of investigational antibody drugs and biosimilars targeting angiogenesis have obtained regulatory approval for the treatment of various malignancies. Moreover, a new generation of bispecific antibodies based on the principle of antiangiogenesis are being advanced for clinical trial to overcome antiangiogenic resistance in tumor treatment or enhance the efficacy of monotherapy. Tumors often develop resistance to antiangiogenesis therapy, presenting as refractory and sometimes even resistant to new therapies, for which there are currently no effective management strategies. Thus, a detailed understanding of the mechanisms mediating resistance to antiangiogenesis antibodies is crucial for improving drug effectiveness and achieving a durable response to antiangiogenic therapy. In this review, we provide a novel perspective on the tumor microenvironment, including antibody structure, tumor stroma, and changes within tumor cells, to analyze the multifactorial reasons underlying resistance to antiangiogenesis antibodies. The review also enumerates biomarkers that indicate resistance and potential strategies for monitoring resistance. Furthermore, based on recent clinical and preclinical studies, we summarize potential strategies and translational clinical trials aimed at overcoming resistance to antiangiogenesis antibodies. This review provides a valuable reference for researchers and clinical practitioners involved in the development of new drugs or therapeutic strategies to overcome antiangiogenesis antibodies resistance.
Recurrence rates and risk factors in obscure gastrointestinal bleeding
Authors: Onishi Sachiyo , Iwashita Takuji , Tezuka Yukari , Kojima Kentaro , Takada Jun , Kubota Masaya , Ibuka Takashi , Shimizu Masahito 
Published: September 2024

AbstractObscure gastrointestinal bleeding (OGIB), defined in 2010, involves bleeding from the GI tract that remains unexplained after standard diagnostic procedures. OGIB, which accounts for about 5% of all GI bleeds, poses diagnostic and management challenges, particularly due to the anatomical features of the small intestine. Advances in capsule endoscopy (CE) and balloon‐assisted endoscopy have improved the diagnostic and therapeutic landscape for small intestinal lesions.ObjectiveTo determine the recurrence rate and identify risk factors for recurrence following diagnostic and therapeutic interventions using CE and balloon‐assisted endoscopy in patients with OGIB.MethodsA retrospective cohort study at Gifu University Hospital analyzed CE procedures for patients with OGIB from 2008 to 2022. Patients underwent CE with subsequent treatments based on the findings. Statistical analyses, including Kaplan‐Meier and Cox proportional hazards models, were used to estimate cumulative recurrence rates and identify recurrence risk factors.ResultsOut of 417 patients, 65.2% had positive CE findings, leading to therapeutic interventions in 16.3% of cases. The cumulative recurrence rates at 12, 24, and 36 months were 4.3%, 9.0%, and 13.9%, respectively. Liver cirrhosis (hazard rate: 4.15, 95% confidence interval 1.88–9.18, p < 0.01) was identified as a significant risk factor for recurrence.ConclusionsA significant recurrence rate in OGIB patients, with liver cirrhosis being a major risk factor. Despite diagnostic and therapeutic advances, a comprehensive approach including careful follow‐up and consideration of risk factors is essential for management.
Testosterone and estradiol reduce inflammation of human macrophages induced by anti‐SARS‐CoV‐2 IgG
Authors: Allahverdiyeva Sona , Geyer Chiara E. , Veth Jennifer , de Vries Laura M. , de Taeye Steven W. , van Gils Marit J. , den Dunnen Jeroen , Chen Hung‐Jen 
Published: September 2024

AbstractCOVID‐19, the disease caused by SARS‐CoV‐2, particularly causes severe inflammatory disease in elderly, obese, and male patients. Since both aging and obesity are associated with decreased testosterone and estradiol expression, we hypothesized that decreased hormone levels contribute to excessive inflammation in the context of COVID‐19. Previously, we and others have shown that hyperinflammation in severe COVID‐19 patients is induced by the production of pathogenic anti‐spike IgG antibodies that activate alveolar macrophages. Therefore, we developed an in vitro assay in which we stimulated human macrophages with viral stimuli, anti‐spike IgG immune complexes, and different sex hormones. Treatment with levels of testosterone reflecting young adults led to a significant reduction in TNF and IFN‐γ production by human macrophages. In addition, estradiol significantly attenuated the production of a very broad panel of cytokines, including TNF, IL‐1β, IL‐6, IL‐10, and IFN‐γ. Both testosterone and estradiol reduced the expression of Fc gamma receptors IIa and III, the two main receptors responsible for anti‐spike IgG‐induced inflammation. Combined, these findings indicate that sex hormones reduce the inflammatory response of human alveolar macrophages to specific COVID‐19‐associated stimuli, thereby providing a potential immunological mechanism for the development of severe COVID‐19 in both older male and female patients.
Ligand‐Directed Assembly of Multimetallic Aluminum Complexes: Synthesis, Structure and ROP Catalysis
Authors: García‐López José‐David , García‐Álvarez Ana‐Cristina , Hernández‐Balderas Uvaldo , Gallardo‐Garibay Alfonso , Jancik Vojtech , Martínez‐Otero Diego , Moya‐Cabrera Mónica 
Published: September 2024

AbstractMultinuclear aluminum complexes are useful catalysts for the ring opening polymerization (ROP) of ϵ‐caprolactone (ϵ‐CL), often outperforming monometallic analogues in the production of biodegradable polycaprolactone (PCL). These complexes require control over metal‐metal distance, ligand structure, and substituents. Herein, we report on the preparation of mono‐, bi‐, and octametallic aluminum complexes through direct chelation of multidentate bis(phosphino)‐2,2‐dimethyl‐1,3‐diamino ligands Me2C[CH2NHR2P(O)]2; R=Ph(1), iPr(2), tBu(3). Ligands 1–3 yielded bimetallic complexes with two types of coordination pockets, as well as bimetallic systems with identical coordination environments. In addition to the diversity of structural patterns within the present series, the catalytic performances of [AlR2{κ2‐N,N’,–(Me2C{CH2NPh2P(O)}2}‐(κ2‐O,O’‐AlR2)] (R=Me(4), Et(5), iBu(6)) outperformed that of the monometallic [AlEt2{κ2‐O,O’‐(Me2C(CH2NtBu2P(O))2H} (10), showing controlled and immortal ROP of ϵ‐CL in the presence of BnOH as co‐initiator. DFT calculations suggest that the primary active site for 4–6 is the N−Al–N center. Gaining insight into the underlying principles that lead to high activity and controlled production of PCL in bimetallic systems can help design tailor‐made complexes with enhanced performance.
Vicinal Difunctionalization of Unactivated Alkenes Through Radical Addition/Remote (Hetero)Aryl Migration Cascade
Authors: Meyer Sébastien , Claraz Aurélie 
Published: September 2024

AbstractAlkenes are ubiquitous in natural and synthetic molecules and constitute versatile building blocks. Remarkably, their radical 1,2‐difunctionalization offers a valuable synthetic tool for rapidly accessing multifunctional compounds through a step and atom‐ economy process. More particularly, the addition of (hetero)aromatic groups onto alkenes is of particular appeal given the numerous applications of aromatic compounds in various fields. However, such process becomes difficult when attempted on simple non‐conjugated aliphatic alkenes (unactivated alkenes). In this context, the (hetero)arylation of unactivated alkenes through the radical functionalization followed by remote (hetero)aryl migration has recently emerged as a powerful opportunity to address the lack of reactivity of such π systems. The advances in radical chemistry have led to a flood of synthetic methods to trigger the addition of a carbon‐ or heteroatom‐centered radical/(hetero)aryl migration sequence. Interestingly, the migration of the aromatic ring can take place from various atoms. This feature has spurred the design of several substrates allowing such a process to occur. This review intends to provide an overview of the contributions according to the type of substrates that have been employed. The reaction scope, limitations and mechanistic aspect will be discussed with the aim of stimulating efforts in this research area.