bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
Authors: Sala-Vila Aleix , Navarro-Lérida Inmaculada , Sánchez-Alvarez Miguel , Bosch Marta , Calvo Carlos , López Juan Antonio , Calvo Enrique , Ferguson Charles , Giacomello Marta , Serafini Annalisa , Scorrano Luca , Enriquez José Antonio , Balsinde Jesús , Parton Robert G. , Vázquez Jesús , Pol Albert , Del Pozo Miguel A. 
Published: June 2016

AbstractThe mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.
Gain of BDNF Function in Engrafted Neural Stem Cells Promotes the Therapeutic Potential for Alzheimer’s Disease
Authors: Wu Cheng-Chun , Lien Cheng-Chang , Hou Wen-Hsien , Chiang Po-Min , Tsai Kuen-Jer 
Published: June 2016

AbstractStem cell-based therapy is a potential treatment for neurodegenerative diseases, but its application to Alzheimer’s disease (AD) remains limited. Brain-derived neurotrophic factor (BDNF) is critical in the pathogenesis and treatment of AD. Here, we present a novel therapeutic approach for AD treatment using BDNF-overexpressing neural stem cells (BDNF-NSCs). In vitro, BDNF overexpression was neuroprotective to beta-amyloid-treated NSCs. In vivo, engrafted BDNF-NSCs-derived neurons not only displayed the Ca2+-response fluctuations, exhibited electrophysiological properties of mature neurons and integrated into local brain circuits, but recovered the cognitive deficits. Furthermore, BDNF overexpression improved the engrafted cells’ viability, neuronal fate, neurite complexity, maturation of electrical property and the synaptic density. In contrast, knockdown of the BDNF in BDNF-NSCs diminished stem cell-based therapeutic efficacy. Together, our findings indicate BDNF overexpression improves the therapeutic potential of engrafted NSCs for AD via neurogenic effects and neuronal replacement and further support the feasibility of NSC-based ex vivo gene therapy for AD.
Quantitative volcanic susceptibility analysis of Lanzarote and Chinijo Islands based on kernel density estimation via a linear diffusion process
Authors: Galindo I. , Romero M. C. , Sánchez N. , Morales J. M. 
Published: June 2016

AbstractRisk management stakeholders in high-populated volcanic islands should be provided with the latest high-quality volcanic information. We present here the first volcanic susceptibility map of Lanzarote and Chinijo Islands and their submarine flanks based on updated chronostratigraphical and volcano structural data, as well as on the geomorphological analysis of the bathymetric data of the submarine flanks. The role of the structural elements in the volcanic susceptibility analysis has been reviewed: vents have been considered since they indicate where previous eruptions took place; eruptive fissures provide information about the stress field as they are the superficial expression of the dyke conduit; eroded dykes have been discarded since they are single non-feeder dykes intruded in deep parts of Miocene-Pliocene volcanic edifices; main faults have been taken into account only in those cases where they could modified the superficial movement of magma. The application of kernel density estimation via a linear diffusion process for the volcanic susceptibility assessment has been applied successfully to Lanzarote and could be applied to other fissure volcanic fields worldwide since the results provide information about the probable area where an eruption could take place but also about the main direction of the probable volcanic fissures.
Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride
Authors: Wang Huan , Wang Yaoguang , Zhang Yong , Wang Qi , Ren Xiang , Wu Dan , Wei Qin 
Published: June 2016

AbstractCarcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO2 nanosheets and carboxylated graphitic carbon nitride (g-C3N4) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO2 nanosheets was sythsized by surfactant self-assembly method and proved to have higher photoelectrochemical signals than TiO2 nanoparticles. Firstly, carboxylated g-C3N4 could be attached to 2D TiO2 nanosheets through the bond formed between carboxyl group of carboxylated g-C3N4 and TiO2. And the photocurrent of g-C3N4/TiO2 drastically enhances compared to carboxylated g-C3N4 and TiO2. Then, antibody of CEA was bonded to TiO2 through the dentate bond formed between carboxyl group of anti-CEA and TiO2, leading to the decrease of the photocurrents. As proven by PEC experiments and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the intensity decreased linearly with CEA concentration in the range of 0.01~10 ng/mL. The detection limit is 2.1 pg/mL. The work provides an effective method for the detection of tumor markers and can be extended for the application in food safety and environmental monitoring analysis.
Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure
Authors: Iijima Yoko , Behr Katharina , Iijima Takatoshi , Biemans Barbara , Bischofberger Josef , Scheiffele Peter 
Published: June 2016

AbstractAutism spectrum disorders (ASDs) are a heterogeneous group of neurodevelopmental disorders characterized by impairments in social interactions and stereotyped behaviors. Valproic acid (VPA) is frequently used to treat epilepsy and bipolar disorders. When taken during pregnancy, VPA increases the risk of the unborn child to develop an ASD. In rodents, in utero VPA exposure can precipitate behavioral phenotypes related to ASD in the offspring. Therefore, such rodent models may allow for identification of synaptic pathophysiology underlying ASD risk. Here, we systematically probed alterations in synaptic proteins that might contribute to autism-related behavior in the offspring of in utero VPA-exposed mice. Moreover, we tested whether direct VPA exposure of cultured neocortical neurons may recapitulate the molecular alterations seen in vivo. VPA-exposed neurons in culture exhibit a significant increase in the number of glutamatergic synapses accompanied by a significant decrease in the number of GABAergic synapses. This shift in excitatory/inhibitory balance results in substantially increased spontaneous activity in neuronal networks arising from VPA-exposed neurons. Pharmacological experiments demonstrate that the alterations in GABAergic and glutamatergic synaptic proteins and structures are largely caused by inhibition of histone deacetylases. Therefore, our study highlights an epigenetic mechanism underlying the synaptic pathophysiology in this ASD model.
Silica ecosystem for synergistic biotransformation
Authors: Mutlu Baris R. , Sakkos Jonathan K. , Yeom Sujin , Wackett Lawrence P. , Aksan Alptekin 
Published: June 2016

AbstractSynergistical bacterial species can perform more varied and complex transformations of chemical substances than either species alone, but this is rarely used commercially because of technical difficulties in maintaining mixed cultures. Typical problems with mixed cultures on scale are unrestrained growth of one bacterium, which leads to suboptimal population ratios and lack of control over bacterial spatial distribution, which leads to inefficient substrate transport. To address these issues, we designed and produced a synthetic ecosystem by co-encapsulation in a silica gel matrix, which enabled precise control of the microbial populations and their microenvironment. As a case study, two greatly different microorganisms: Pseudomonas sp. NCIB 9816 and Synechococcus elongatus PCC 7942 were encapsulated. NCIB 9816 can aerobically biotransform over 100 aromatic hydrocarbons, a feat useful for synthesis of higher value commodity chemicals or environmental remediation. In our system, NCIB 9816 was used for biotransformation of naphthalene (a model substrate) into CO2 and the cyanobacterium PCC 7942 was used to provide the necessary oxygen for the biotransformation reactions via photosynthesis. A mathematical model was constructed to determine the critical cell density parameter to maximize oxygen production and was then used to maximize the biotransformation rate of the system.
Enhanced ordering reduces electric susceptibility of liquids confined to graphene slit pores
Authors: Terrones Jeronimo , Kiley Patrick J. , Elliott James A. 
Published: June 2016

AbstractThe behaviours of a range of polar and non-polar organic liquids (acetone, ethanol, methanol, N-methyl-2-pyrrolidone (NMP), carbon tetrachloride and water) confined to 2D graphene nanochannels with thicknesses in the range of 4.5 Å to 40 Å were studied using classical molecular dynamics and hybrid density functional theory. All liquids were found to organise spontaneously into ordered layers parallel to the confining surfaces, with those containing polar molecules having their electric dipoles aligned parallel to such surfaces. In particular, monolayers of NMP showed remarkable in-plane ordering and low molecular mobility, suggesting the existence of a previously unknown 2D solid-like phase. Calculations for polar liquids showed dramatically reduced static permittivities normal to the confining surfaces; these changes are expected to improve electron tunnelling across the liquid films, modifying the DC electrical properties of immersed assemblies of carbon nanomaterials.
Impermeable flexible liquid barrier film for encapsulation of DSSC metal electrodes
Authors: Yang Junghee , Min Misook , Yoon Yeoheung , Kim Won Jung , Kim Sol , Lee Hyoyoung 
Published: June 2016

AbstractEncapsulation of electronic devices such as dye-sensitized solar cells (DSSCs) is prone to degradation under normal atmospheric conditions, even with hermetic barriers on the metal electrodes. Overcoming this problem is crucial to increasing DSSC lifetimes and making them commercially viable. Herein, we report a new impermeable flexible liquid barrier film using polyvinyl alcohol (PVA) and partially reduced graphene oxide (PrGO), which dramatically enhances the lifetime of Ag metal electrodes (typically used in DSSCs) immersed in a highly acidic iodolyte solution. The Ag metal electrode encapsulated by the PVA/PrGO film survived for over 500 hrs, superior to existing barriers of glass frits, epoxy resins and polymers. The PVA/PrGO film strongly adheres to the Ag metal surface, and the resulting PVA/PrGO/Ag electrode is stable even on a curved substrate, with a sheet resistance nearly independent of curvature. These results give new insight for the design of high-performance and solution-processable flexible liquid barrier films for a wide range of applications, in particular for the encapsulation of electronic devices with liquid electrolytes.
Exceptional high fatigue strength in Cu-15at.%Al alloy with moderate grain size
Authors: Liu Rui , Tian Yanzhong , Zhang Zhenjun , An Xianghai , Zhang Peng , Zhang Zhefeng 
Published: June 2016

AbstractIt is commonly proposed that the fatigue strength can be enhanced by increasing the tensile strength, but this conclusion needs to be reconsidered according to our study. Here a recrystallized α-Cu-15at.%Al alloy with moderate grain size of 0.62 μm was fabricated by cold rolling and annealing, and this alloy achieved exceptional high fatigue strength of 280 MPa at 107 cycles. This value is much higher than the fatigue strength of 200 MPa for the nano-crystalline counterpart (0.04 μm in grain size) despite its higher tensile strength. The remarkable improvement of fatigue strength should be mainly attributed to the microstructure optimization, which helps achieve the reduction of initial damage and the dispersion of accumulated damage. A new strategy of “damage reduction” was then proposed for fatigue strength improvement, to supplement the former strengthening principle. The methods and strategies summarized in this work offer a general pathway for further improvement of fatigue strength, in order to ensure the long-term safety of structural materials.
Shedding light on Aspergillus niger volatile exometabolome
Authors: Costa Carina Pedrosa , Gonçalves Silva Diogo , Rudnitskaya Alisa , Almeida Adelaide , Rocha Sílvia M. 
Published: June 2016

AbstractAn in-depth exploration of the headspace content of Aspergillus niger cultures was performed upon different growth conditions, using a methodology based on advanced multidimensional gas chromatography. This volatile fraction comprises 428 putatively identified compounds distributed over several chemical families, being the major ones hydrocarbons, alcohols, esters, ketones and aldehydes. These metabolites may be related with different metabolic pathways, such as amino acid metabolism, biosynthesis and metabolism of fatty acids, degradation of aromatic compounds, mono and sesquiterpenoid synthesis and carotenoid cleavage. The A. niger molecular biomarkers pattern was established, comprising the 44 metabolites present in all studied conditions. This pattern was successfully used to distinguish A. niger from other fungi (Candida albicans and Penicillium chrysogenum) with 3 days of growth by using Partial Least Squares-Discriminant Analysis (PLS-DA). In addition, PLS-DA-Variable Importance in Projection was applied to highlight the metabolites playing major roles in fungi distinction; decreasing the initial dataset to only 16 metabolites. The data pre-processing time was substantially reduced and an improvement of quality-of-fit value was achieved. This study goes a step further on A. niger metabolome construction and A. niger future detection may be proposed based on this molecular biomarkers pattern.
Mangrove succession enriches the sediment microbial community in South China
Authors: Chen Quan , Zhao Qian , Li Jing , Jian Shuguang , Ren Hai 
Published: June 2016

AbstractSediment microorganisms help create and maintain mangrove ecosystems. Although the changes in vegetation during mangrove forest succession have been well studied, the changes in the sediment microbial community during mangrove succession are poorly understood. To investigate the changes in the sediment microbial community during succession of mangroves at Zhanjiang, South China, we used phospholipid fatty acid (PLFA) analysis and the following chronosequence from primary to climax community: unvegetated shoal; Avicennia marina community; Aegiceras corniculatum community; and Bruguiera gymnorrhiza + Rhizophora stylosa community. The PLFA concentrations of all sediment microbial groups (total microorganisms, fungi, gram-positive bacteria, gram-negative bacteria, and actinomycetes) increased significantly with each stage of mangrove succession. Microbial PLFA concentrations in the sediment were significantly lower in the wet season than in the dry season. Regression and ordination analyses indicated that the changes in the microbial community with mangrove succession were mainly associated with properties of the aboveground vegetation (mainly plant height) and the sediment (mainly sediment organic matter and total nitrogen). The changes in the sediment microbial community can probably be explained by increases in nutrients and microhabitat heterogeneity during mangrove succession.
Thermal transport in bismuth telluride quintuple layer: mode-resolved phonon properties and substrate effects
Authors: Shao Cheng , Bao Hua 
Published: June 2016

AbstractThe successful exfoliation of atomically-thin bismuth telluride (Bi2Te3) quintuple layer (QL) attracts tremendous research interest in this strongly anharmonic quasi-two-dimensional material. The thermal transport properties of this material are not well understood, especially the mode-wise properties and when it is coupled with a substrate. In this work, we have performed molecular dynamics simulations and normal mode analysis to study the mode-resolved thermal transport in freestanding and supported Bi2Te3 QL. The detailed mode-wise phonon properties are calculated and the accumulated thermal conductivities with respect to phonon mean free path (MFP) are constructed. It is shown that 60% of the thermal transport is contributed by phonons with MFP longer than 20 nm. Coupling with a-SiO2 substrate leads to about 60% reduction of thermal conductivity. Through varying the interfacial coupling strength and the atomic mass of substrate, we also find that phonon in Bi2Te3 QL is more strongly scattered by interfacial potential and its transport process is less affected by the dynamics of substrate. Our study provides an in-depth understanding of heat transport in Bi2Te3 QL and is helpful in further tailoring its thermal property through nanostructuring.
Postoperative improvement in DASH score, clinical findings, and nerve conduction velocity in patients with cubital tunnel syndrome
Authors: Ido Yoshikazu , Uchiyama Shigeharu , Nakamura Koichi , Itsubo Toshiro , Hayashi Masanori , Hata Yukihiko , Imaeda Toshihiko , Kato Hiroyuki 
Published: June 2016

AbstractWe investigated a recovery pattern in subjective and objective measures among 52 patients with cubital tunnel syndrome after anterior subcutaneous transposition of the ulnar nerve. Disabilities of the Arm, Shoulder and Hand (DASH) score (primary outcome), numbness score, grip and pinch strength, Semmes-Weinstein (SW) score, static 2-point discrimination (2PD) score, and motor conduction velocity (MCV) stage were examined preoperatively and 1, 3, 6, 12, and ≥24 months postoperatively. Statistical analyses were conducted to evaluate how each variable improved after surgery. A linear mixed-effects model was used for continuous variables (DASH score, numbness, grip and pinch strength), and a proportional odds model was used for categorical variables (SW and 2PD tests and MCV stages). DASH score significantly improved by 6 months. Significant recovery in numbness and SW test scores occurred at 1 month. Grip and pinch strength, 2PD test scores, and MCV stage improved by 3 months. DASH scores and numbness recovered regardless of age, sex, or disease severity. It was still unclear if both subjective and objective measures improved beyond 1-year postoperatively. These data are helpful for predicting postoperative recovery patterns and tend to be most important for patients prior to surgery.
„Man muss das Rad nicht neu erfinden, sondern damit fahren.“ – Voraussetzungen für einen erfolgreichen Projekttransfer
Authors: Alfes J. , Schaefer I. , Kolip P. 
Published: June 2016

ZusammenfassungObgleich die Weitergabe eines bereits bestehenden Projekts eine ressourcensparende Form der Implementation bewährter Interventionen darstellen kann, finden in der Alltagspraxis bislang nur wenige Projekte Nachahmer und Anwender. Ziel des vorliegenden Artikels ist die Bündelung der Erfahrungen dreier Modellvorhaben mit der Übertragung ihrer Präventionsansätze in andere Kommunen und Regionen sowie die Ableitung von Empfehlungen für die Gestaltung zukünftiger überregionaler Transferprozesse im Bereich der Prävention und Gesundheitsförderung. Dazu wurden qualitative Interviews mit Verantwortlichen der Modellvorhaben sowie der Transferregionen geführt und Dokumentationsbögen eingesetzt. Die Ergebnisse zeigen, dass die Möglichkeit, die Intervention an die spezifischen Gegebenheiten der Transferregion anzupassen, eine bedeutsame Gelingensbedingung für einen Projekttransfer darstellt. Daneben tragen ein bestehender Bedarf für die Intervention in der Transferregion, ein erkennbarer Nutzen für die projektnehmende Organisation, eine konstruktive und persönliche Kommunikation sowie eine partizipative Zusammenarbeit zwischen den projektgebenden und projektnehmenden Organisationen wesentlich zu einem erfolgreichen Projekttransfer bei.
On the shoulders of giants: undertaking a structured literature review in accounting
Authors: Massaro Maurizio , Dumay John , Guthrie James 
Published: June 2016

Purpose– The purpose of this paper is to present a method for a structured literature review (SLR). An SLR is a method for examining a corpus of scholarly literature, to develop insights, critical reflections, future research paths and research questions. SLRs are common in scientific disciplines dominated by quantitative approaches, but they can be adapted in accounting studies since quantitative and qualitative approaches are commonly accepted.Design/methodology/approach– A literature review, as a piece of academic writing, must have a logical, planned structure. The authors also argue it requires tests based on qualitative and quantitative methods. Therefore, the authors describe ten steps for developing an SLR.Findings– The SLR method is a way that scholars can stand “on the shoulders of giants” and provide insightful and impactful research that is different to the traditional authorship approaches to literature reviews.Research limitations/implications– Traditional literature reviews can have varied results because of a lack of rigour. SLRs use a process that, through a set of rules, potentially offers less bias and more transparency of the execution and measures and techniques of validation and reliability.Practical implications– SLRs provide an approach that can help academics to discover under-investigated topics and methods, nurturing, therefore, the development of new knowledge areas and research approaches.Originality/value– The paper presents accounting researchers with an opportunity to develop insightful and publishable studies, and also serves as a basis for developing future research agendas in the accounting field. The authors advocate the SLR method especially to higher degree research students and emerging scholars as a way of potentially developing robust and defensible research agendas and questions.