bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancement of Ebola Virus Infection via Ficolin-1 Interaction with the Mucin Domain of GP Glycoprotein
Authors: Favier Anne-Laure , Gout Evelyne , Reynard Olivier , Ferraris Olivier , Kleman Jean-Philippe , Volchkov Viktor , Peyrefitte Christophe , Thielens Nicole M. 
Published: March 2016

ABSTRACT
Ebola virus infection requires the surface viral glycoprotein to initiate entry into the target cells. The trimeric glycoprotein is a highly glycosylated viral protein which has been shown to interact with host C-type lectin receptors and the soluble complement recognition protein mannose-binding lectin, thereby enhancing viral infection. Similarly to mannose-binding lectin, ficolins are soluble effectors of the innate immune system that recognize particular glycans at the pathogen surface. In this study, we demonstrate that ficolin-1 interacts with the Zaire Ebola virus (EBOV) glycoprotein, and we characterized this interaction by surface plasmon resonance spectroscopy. Ficolin-1 was shown to bind to the viral glycoprotein with a high affinity. This interaction was mediated by the fibrinogen-like recognition domain of ficolin-1 and the mucin-like domain of the viral glycoprotein. Using a ficolin-1 control mutant devoid of sialic acid-binding capacity, we identified sialylated moieties of the mucin domain to be potential ligands on the glycoprotein. In cell culture, using both pseudotyped viruses and EBOV, ficolin-1 was shown to enhance EBOV infection independently of the serum complement. We also observed that ficolin-1 enhanced EBOV infection on human monocyte-derived macrophages, described to be major viral target cells,. Competition experiments suggested that although ficolin-1 and mannose-binding lectin recognized different carbohydrate moieties on the EBOV glycoprotein, the observed enhancement of the infection likely depended on a common cellular receptor/partner. In conclusion, ficolin-1 could provide an alternative receptor-mediated mechanism for enhancing EBOV infection, thereby contributing to viral subversion of the host innate immune system.

IMPORTANCE
A specific interaction involving ficolin-1 (M-ficolin), a soluble effector of the innate immune response, and the glycoprotein (GP) of EBOV was identified. Ficolin-1 enhanced virus infection instead of tipping the balance toward its elimination. An interaction between the fibrinogen-like recognition domain of ficolin-1 and the mucin-like domain of Ebola virus GP occurred. In this model, the enhancement of infection was shown to be independent of the serum complement. The facilitation of EBOV entry into target host cells by the interaction with ficolin-1 and other host lectins shunts virus elimination, which likely facilitates the survival of the virus in infected host cells and contributes to the virus strategy to subvert the innate immune response.
Adverse drug reactions in neonates: a prospective study
Authors: Belén Rivas Ana , Arruza Luis , Pacheco Enrique , Portoles Antonio , Diz Jorge , Vargas Emilio 
Published: March 2016

AimTo investigate the frequency and characteristics of adverse drug reactions (ADRs) in hospitalised neonates to obtain a better understanding of and improvement in neonatal healthcare.MethodologyA prospective cohort study. Data were collected on 313 neonates and 2166 drug prescriptions. Clinical characteristics of patients, drugs administered and ADRs were prospectively recorded and analysed. Informed consent was obtained in all cases.Results116 different ADRs were detected. 17% of the neonates experienced at least one of these ADRs. Systemic antimicrobials and caffeine citrate were the drugs that most commonly caused ADRs. According to the ADR Severity Assessment Scale, 41% were mild, 42% were moderate and 17% were severe. Of the ADRs identified, 11% were classified as ‘certain’ by the Naranjo method and 20% were classified as ‘defined’ by the Karch and Lasagna modified algorithm. Most of the ADRs detected were related to feed intolerance, phlebitis and tachycardia. Most were acute (73%) and lasted between 1 and 7 days (39%). After the occurrence of an ADR, it was necessary to initiate specific treatment in 44 cases, discontinue the drugs involved in 30 cases, and reduce the drug dose in another 30 cases. An association was shown between the number of drugs prescribed and ADR onset.ConclusionsThere is a high incidence of ADRs in hospitalised newborns, which increases with the number of prescriptions.
Role of community pharmacists in the use of antipsychotics for behavioural and psychological symptoms of dementia (BPSD): a qualitative study
Authors: Maidment Ian D , Aston Lydia , Hilton Andrea , Iqbal Naveed , Child Anne , Shaw Rachel 
Published: March 2016

ObjectiveThis study aimed to use qualitative methodology to understand the current role of community pharmacists in limiting the use of antipsychotics prescribed inappropriately for behavioural and psychological symptoms of dementia.DesignA qualitative study employing focus groups was conducted. Data were analysed using thematic analysis.Setting3 different geographical locations in the England.ParticipantsCommunity pharmacists (n=22).ResultsThe focus groups identified an array of factors and constraints, which affect the ability of community pharmacists to contribute to initiatives to limit the use of antipsychotics. 3 key themes were revealed: (1) politics and the medical hierarchy, which created communication barriers; (2) how resources and remit impact the effectiveness of community pharmacy; and (3) understanding the nature of the treatment of dementia.ConclusionsOur findings suggest that an improvement in communication between community pharmacists and healthcare professionals, especially general practitioners (GPs) must occur in order for community pharmacists to assist in limiting the use of antipsychotics in people with dementia. Additionally, extra training in working with people with dementia is required. Thus, an intervention which involves appropriately trained pharmacists working in collaboration with GPs and other caregivers is required. Overall, within the current environment, community pharmacists question the extent to which they can contribute in helping to reduce the prescription of antipsychotics.
Qualitative study to conceptualise a model of interprofessional collaboration between pharmacists and general practitioners to support patients' adherence to medication
Authors: Rathbone Adam P , Mansoor Sarab M , Krass Ines , Hamrosi Kim , Aslani Parisa 
Published: March 2016

ObjectivesPharmacists and general practitioners (GPs) face an increasing expectation to collaborate interprofessionally on a number of healthcare issues, including medication non-adherence. This study aimed to propose a model of interprofessional collaboration within the context of identifying and improving medication non-adherence in primary care.SettingPrimary care; Sydney, Australia.Participants3 focus groups were conducted with pharmacists (n=23) and 3 with GPs (n=22) working in primary care.Primary and secondary outcome measuresQualitative investigation of GP and pharmacist interactions with each other, and specifically around supporting their patients’ medication adherence. Audio-recordings were transcribed verbatim and transcripts thematically analysed using a combination of manual and computer coding.Results3 themes pertaining to interprofessional collaboration were identified (1) frequency, (2) co-collaborators and (3) nature of communication which included 2 subthemes (method of communication and type of communication). While the frequency of interactions was low, the majority were conducted by telephone. Interactions, especially those conducted face-to-face, were positive. Only a few related to patient non-adherence. The findings are positioned within contemporary collaborative theory and provide an accessible introduction to models of interprofessional collaboration.ConclusionsThis work highlighted that successful collaboration to improve medication adherence was underpinned by shared paradigmatic perspectives and trust, constructed through regular, face-to-face interactions between pharmacists and GPs.
Postharvest performance of cut ‘White Prosperity’ gladiolus spikes in response to nano- and other silver sources
Authors: Ahmad Iftikhar , Saleem Muhammad , Dole John M. 
Published: March 2016

A study was conducted to compare the efficacy of nano-silver (NS), silver thiosulfate (STS), and silver nitrate as novel postharvest treatments, and evaluate the potential of nano-silver pulse (higher concentration) or vase (lower concentration) applications on postharvest performance of cut gladiolus (Gladiolus hybrids ‘White Prosperity’) spikes. Among silver compounds, spikes kept in silver nitrate had longest vase life. Spikes placed in STS at 10 or 20 mg L−1 had longer vase life than stems placed in NS, but similar to the stems placed in water. For NS pulse applications, spikes pulsed with 50 mg L−1 NS for 1 h at 20 ± 2 °C had 2.2 d longer vase life compared to the spikes in water. Pulsing of spikes for 1 h extended the vase life of cut gladiolus compared with 4 or 8 h pulsing durations. Spikes kept continuously in NS until end of vase life resulted in 4.2 d longer vase life at a lower concentration of 0.01 mg L−1. With increasing NS concentration to 10 mg L−1, vase life decreased to similar vase life as with tap water. The results demonstrated that NS at a 50 mg L−1 pulse for 1 h or 0.01 mg L−1 vase application can be used for extending vase life of cut gladiolus spikes.
Physical properties of an Orthic Black Chernozem after 5 years of liquid and solid pig manure application to annual and perennial crops
Authors: Adesanya Theresa , Akinremi Olalekan , Zvomuya Francis 
Published: March 2016

Pig (Sus scrofa) manure is added to the soil to supply nutrients and improve soil properties. To our knowledge, no direct comparison has been made on the effect of liquid pig manure (LPM) and solid pig manure (SPM) on the physical properties of a prairie soil. This study was established in 2009 at the University of Manitoba’s Ian Morrison Research Station in Carman, Manitoba. The treatment design was a split-plot structure with cropping system as the main plot and manure treatments as subplots. Five years after the study was initiated, soil samples were collected from the 0–10 cm and 10–20 cm depth intervals for determination of bulk density, saturated hydraulic conductivity (Ksat), and water retention at field capacity and permanent wilting point (PWP). For wet aggregate stability, samples were collected from the 0–5 cm layer. Land application of SPM significantly decreased bulk density by 14%, significantly increased Ksatby 110% in the 0–10 cm layer, and resulted in a 30% increase in wet aggregate stability (P < 0.05). In perennial plots, SPM increased water retention at field capacity, PWP, and available water in the 0–10 cm compared with annual plots. This was not the case for LPM-amended soils. We conclude that SPM has the potential as an organic amendment to improve the physical properties of the topsoil.
Incorporating Computation Time Measures During Heterogeneous Features Selection in a Boosted Cascade People Detector
Authors: Mekonnen Alhayat Ali , Lerasle Frédéric , Herbulot Ariane , Briand Cyril 
Published: March 2016

In this paper, we investigate the notion of incorporating feature computation time (CT) measures during feature selection in a boosted cascade people detector utilizing heterogeneous pool of features. We present various approaches based on pareto-front analysis, CT weighted adaboost, and Binary Integer Programming (BIP) with comparative evaluations. The novel feature selection method proposed based on BIP — the main contribution — mines heterogeneous features taking both detection performance and CT explicitly into consideration. The results demonstrate that the detector using this feature selection scheme exhibits low miss rates (MRs) with significant boost in frame rate. For example, it achieves a [Formula: see text] less MR at [Formula: see text] FPPW compared to Dalal and Triggs HOG detector with a [Formula: see text]x speed improvement. The presented extensive experimental results clearly highlight the improvements the proposed framework brings to the table.
The CoCoME Platform: A Research Note on Empirical Studies in Information System Evolution
Authors: Heinrich Robert , Gärtner Stefan , Hesse Tom-Michael , Ruhroth Thomas , Reussner Ralf , Schneider Kurt , Paech Barbara , Jürjens Jan 
Published: March 2016

Methods for supporting evolution of software-intensive systems are a competitive edge in software engineering as software is often operated over decades. Empirical research is useful to validate the effectiveness of these methods. However, empirical studies on software evolution are rarely comprehensive and hardly replicable. Collaboration may prevent these shortcomings. We designed CoCoMEP — a platform for supporting collaboration in empirical research on software evolution by shared knowledge. We report lessons learned from the application of the platform in a large research programme.
Effect of acoustic pulses and EHF radiation on multipotent marrow stromal cells in tissue engineering constructs
Authors: Chailakhyan R. K. , Yusupov V. I. , Gorskaya Yu. F. , Kuralesova A. I. , Gerasimov Yu. V. , Sviridov A. P. , Tambiev A. Kh. , Vorobieva N. N. , Grosheva A. G. , Shishkova V. V. , Moskvina I. L. , Bagratashvili V. N. 
Published: March 2016

In this paper, we studied the effects of physical factors, such as, acoustic pulses of laser-induced hydrodynamics (ALIH) and extremely-high frequencies (EHF) radiation, on the formation of heterotopic bone marrow organs. A suspension of precipitated bone marrow cells from CBA mice were exposed to ALIH pulses and EHF radiation separately and in their combination tissue engineering constructs, presenting gelatin sponges 2 by 2 by 2[Formula: see text]mm in size containing 107 nucleated bone marrow cells, were exposed to physical factors and were implanted under the renal capsules of syngeneic mice. The newly formed hematopoietic organs were examined in three and five months later after treatment. The five months old transplants were bigger in size than the three months old transplants. The number of hematopoietic cells in the rest of the groups increased during this period by a factor from 3 to 10, the increase being as high as 20-fold in the ALIH[Formula: see text]EHF group. Maximal concentration of multipotent stromal cells (MSCs) was in the EHF[Formula: see text]ALIH, and minimal concentration was in the ALIH[Formula: see text]EHF. The accumulation rate of bone capsule weight was highest for the transplants of EHF[Formula: see text]ALIH and ALIH-sponge groups during the first three months. These data showed that the combined impact of the EHF[Formula: see text]ALIH on MSCs is the most effective for the formation of bone marrow transplantation.
Modelling lava flow advance using a shallow-depth approximation for three-dimensional cooling of viscoplastic flows
Authors: Bernabeu Noé , Saramito Pierre , Smutek Claude 
Published: March 2016

Abstract
A new shallow-depth approximation model for lava flow advance and cooling on a quantized topography is presented in this paper. To apply the model, lava rheology is described using a non-isothermal three-dimensional viscoplastic fluid in which the rheological properties are assumed to be temperature dependent. Asymptotic analysis allows a three-dimensional flow scenario to be reduced to a two-dimensional problem using depth-averaged equations. These equations are numerically approximated by an autoadaptive finite element method, based on the Rheolef C++ library, which allows economy of computational time. Here, the proposed approach is first evaluated by comparing numerical output with non-isothermal experimental results for a flow of silicon oil. Finally, the December 2010 eruption of Piton de la Fournaise (La Réunion Island) is numerically reproduced and compared with available data.
On the detection and monitoring of effusive eruptions using satellite SO
2
measurements
Authors: Carn S. A. 
Published: March 2016

Abstract

Timely detection and quantification of lava effusion rates are crucial for volcanic hazard mitigation during effusive eruptions. Satellite-based detection methods typically exploit the exceptional radiant heat fluxes associated with lava effusion, but effusive eruptions can also emit prodigious amounts of sulphur dioxide (SO
2
). Measuring the magnitude and temporal evolution of SO
2
emissions provides an additional means for monitoring effusive eruptions, complementing thermal monitoring. Examples of effusive eruptions detected since 1978 using ultraviolet (UV) satellite measurements of SO
2
emissions by the Total Ozone Mapping Spectrometer (TOMS), Ozone Monitoring Instrument (OMI) and Ozone Mapping and Profiler Suite (OMPS) are reviewed. During many effusive eruptions, trends in SO
2
production mimic the classic waxing–waning pattern characteristic of such events that is also seen in thermal infrared (TIR) hotspot data, suggesting a qualitative link between SO
2
emissions and lava effusion rates. An example of lava effusion rate calculation based on TOMS SO
2
measurements is presented for the 1998 eruption of Cerro Azul (Galápagos Islands), for which detailed eruption observations and independent estimates of effusion rates are available. Combining TOMS-derived SO
2
emission rates with estimates of sulphur content in Cerro Azul lavas yields lava effusion rates almost identical to independently derived values, demonstrating the utility of the technique.
An Epigenetic Reprogramming Strategy to Resensitize Radioresistant Prostate Cancer Cells
Authors: Peitzsch Claudia , Cojoc Monica , Hein Linda , Kurth Ina , Mäbert Katrin , Trautmann Franziska , Klink Barbara , Schröck Evelin , Wirth Manfred P. , Krause Mechthild , Stakhovsky Eduard A. , Telegeev Gennady D. , Novotny Vladimir , Toma Marieta , Muders Michael , Baretton Gustavo B. , Frame Fiona M. , Maitland Norman J. , Baumann Michael , Dubrovska Anna 
Published: March 2016

Abstract
Radiotherapy is a mainstay of curative prostate cancer treatment, but risks of recurrence after treatment remain significant in locally advanced disease. Given that tumor relapse can be attributed to a population of cancer stem cells (CSC) that survives radiotherapy, analysis of this cell population might illuminate tactics to personalize treatment. However, this direction remains challenging given the plastic nature of prostate cancers following treatment. We show here that irradiating prostate cancer cells stimulates a durable upregulation of stem cell markers that epigenetically reprogram these cells. In both tumorigenic and radioresistant cell populations, a phenotypic switch occurred during a course of radiotherapy that was associated with stable genetic and epigenetic changes. Specifically, we found that irradiation triggered histone H3 methylation at the promoter of the CSC marker aldehyde dehydrogenase 1A1 (ALDH1A1), stimulating its gene transcription. Inhibiting this methylation event triggered apoptosis, promoted radiosensitization, and hindered tumorigenicity of radioresistant prostate cancer cells. Overall, our results suggest that epigenetic therapies may restore the cytotoxic effects of irradiation in radioresistant CSC populations. Cancer Res; 76(9); 2637–51. ©2016 AACR.
An Unbiased Oncology Compound Screen to Identify Novel Combination Strategies
Authors: O'Neil Jennifer , Benita Yair , Feldman Igor , Chenard Melissa , Roberts Brian , Liu Yaping , Li Jing , Kral Astrid , Lejnine Serguei , Loboda Andrey , Arthur William , Cristescu Razvan , Haines Brian B. , Winter Christopher , Zhang Theresa , Bloecher Andrew , Shumway Stuart D. 
Published: March 2016

Abstract
Combination drug therapy is a widely used paradigm for managing numerous human malignancies. In cancer treatment, additive and/or synergistic drug combinations can convert weakly efficacious monotherapies into regimens that produce robust antitumor activity. This can be explained in part through pathway interdependencies that are critical for cancer cell proliferation and survival. However, identification of the various interdependencies is difficult due to the complex molecular circuitry that underlies tumor development and progression. Here, we present a high-throughput platform that allows for an unbiased identification of synergistic and efficacious drug combinations. In a screen of 22,737 experiments of 583 doublet combinations in 39 diverse cancer cell lines using a 4 by 4 dosing regimen, both well-known and novel synergistic and efficacious combinations were identified. Here, we present an example of one such novel combination, a Wee1 inhibitor (AZD1775) and an mTOR inhibitor (ridaforolimus), and demonstrate that the combination potently and synergistically inhibits cancer cell growth in vitro and in vivo. This approach has identified novel combinations that would be difficult to reliably predict based purely on our current understanding of cancer cell biology. Mol Cancer Ther; 15(6); 1155–62. ©2016 AACR.
Percutaneous Common Carotid Artery Access for Pediatric Interventional Cardiac Catheterization
Authors: Justino Henri , Petit Christopher J. 
Published: March 2016

Background—
Surgical cutdown for access to the common carotid artery provides a more direct route for certain pediatric cardiac interventions and avoids femoral artery injury in small infants. The safety of percutaneous carotid access (PCA) in children is unknown.

Methods and Results—
Retrospective review of PCA at Texas Children’s Hospital and Children’s Healthcare of Atlanta. From July 2006 to November 2014, 42 patients underwent 47 attempts at catheterization via PCA. Median (range) age was 20 days (0 days–2.9 years) and weight was 3.2 kg (1.1–12.2). Two patients had failed PCA with no sequelae. Of the 45 catheterizations with successful PCA and sheath placement, 44 interventions were performed, the most common being stenting or stent redilation of the ductus arteriosus, balloon aortic valvuloplasty, and stenting or angioplasty of Blalock–Taussig shunts. After sheath withdrawal, hemostasis was achieved with manual compression, with no need for surgical control of bleeding. Follow-up carotid imaging was performed in all. Acutely, 3 patients developed carotid thrombosis with resolution in 1 and mild residual narrowing in 2 after anticoagulation therapy. At follow-up, 40 of 42 patients (95%) had a normal carotid artery, with 2 instances of mild stenosis. There were no neurological sequelae attributable to PCA.

Conclusions—
PCA is safe even in small infants, and hemostasis can be achieved without surgical repair, with a carotid patency rate superior to published data after surgical cutdown. Surgical cutdown is not routinely required for pediatric cardiac catheterization via the carotid artery.