bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Electrochemical Method for Monitoring the Acidity of Water for Fuel Cell and Environmental Applications
Authors: Gao Xiangming , Dai Chencheng , Xu Zhichuan J. , Lawrence Nathan S. , Fisher Adrian C. 
Published: November 2017

AbstractMaintaining energy and water supplies is still a prime global challenge and hydrogen fuels are set to offer a solution to this challenge, as the combination of hydrogen and oxygen as a fuel stock with the generation of pure water as by‐product is extremely attractive. However, ensuring that the water supply is clean and contaminant‐free is key to ensuring that it can be incorporated into the drinking supply. Contaminants from the fuel cell catalysts or breakdown of materials within the fuel cell over its working life will affect the water quality. The measurement of acidity (pH) is an important indicator for understanding water quality. This article reports a calibration‐free, affordable, screen‐printed electrode (SPE) capable of measuring pH within these waters. This device is fabricated by screen printing pH‐insensitive ferrocene, in conjunction with a pH‐active (and redox‐active) alizarin compound. This electrode reveals consistent voltammetric response to both buffered and low‐buffered media with sufficient supporting electrolytes, achieving a working range from pH 2 to 9.5. This approach provides a disposable, reproducible, low‐cost method for pH monitoring in harsh environments, for example in low‐buffered water quality monitoring and the food industry.
Ractopamine in particulate matter emitted from beef cattle feedyards and playa wetlands in the Central Plains
Authors: Wooten Kimberly J. , Sandoz Melissa A. , Smith Philip N. 
Published: November 2017

Abstract

Beef cattle in the United States are routinely administered ractopamine, a β-adrenergic receptor agonist, to enhance growth. The present study is the first to quantify ractopamine in feedyard-emitted particulate matter and playa wetlands near feedyards. Ractopamine was present in 92% of particulate matter samples, 16% of playa sediment samples, and 3% of playa water samples, at maximum concentrations of 4.7 μg/g, 5.2 ng/g (dry wt), and 271 ng/L, respectively. These data suggest that aerial transmission and deposition of particulate matter is a transport mechanism for ractopamine between feedyards and aquatic systems in the region. Environ Toxicol Chem 2018;37:970–974. © 2017 SETAC
Finger gesture input utilizing the rear camera of a mobile phone: A perspective of mobile CAD
Authors: Lee Sangwon , Ha Taehyun , Lim Seong‐Jae 
Published: November 2017

AbstractVarious interfaces have been suggested for mobile devices, including touch gesture and embedded sensor‐based interfaces. However, if a user's task requires a thorough look, these interfaces hinder sight of view in display and thus can be inappropriate for the tasks. This problem is more important especially in the mobile computer aided design (CAD) context, which performs visually demanding tasks on the limited screen. To address this point, this study suggests a mobile interface utilizing the rear camera of the device and describes the function mapping for CAD. The suggested interface detects finger attachment and movement direction. The number of colors in camera vision and local binary pattern are used as features, and a support vector machine (SVM) is used for feature classification. A prototype application is designed for validation, and appropriate SVM models are selected through benchmarking tests. The validation results show that the suggested interface can perform with high accuracy and low computational resources.
High HSF4 expression is an independent indicator of poor overall survival and recurrence free survival in patients with primary colorectal cancer
Authors: Yang Yingchi , Jin Lan , Zhang Jinghui , Wang Jin , Zhao Xiaomu , Wu Guocong , Yao Hongwei , Zhang Zhongtao 
Published: November 2017

AbstractHeat shock factor 4 (HSF4) is a member of the HSF family. In this study, by using data from the Cancer Genome Atlas‐Colorectal Cancer (TCGA‐CRC), we investigated the expression profile and the prognostic value of the HSF4 in terms of overall survival (OS) and recurrence free survival (RFS) in CRC patients. RNA‐Seq data showed that HSF4 RNA expression was significantly higher in CRC tissues (N = 380) than in the corresponding normal tissues (N = 51) (mean ± SD: 3.56 ± 1.28 vs. 1.85 ± 0.87, P < 0.0001). High HSF4 expression group had significantly higher ratio of stages III/IV patients (52/86, 60.5%) than low HSF4 expression group (110/264, 41.7%; P = 0.0024). Besides, the high HSF4 expression group also had significantly increased expression of CEA (CEA ≥ 5, 26/51, 51.0% vs. 64/186, 34.4%), higher proportion of recurrence (32/86, 37.2% vs. 48/254, 18.9%, P = 0.0005) and death (36/90, 40.0% vs. 49/277, 17.7%, P < 0.0001) compared with the low HSF4 expression group. Multivariate analysis confirmed that high HSF4 expression was an independent prognostic factor of poor OS (HR = 2.111, 95%CI: 1.350–3.302, P = 0.001) and RFS (HR = 1.958, 95%CI: 1.224–3.131, P = 0.005). Bioinformatic analysis showed that HSF4 can directly interact with DUSP26, ZBED8, and MAPK14. It is also coexpressed with PTGER1, COL11A2, CLPS, and ARSA and colocalized with PTGER1, ADRB1, PEX12, CLPS, PSEN2, KCNJ5, CPA1, ARSA, PNLIP, IRX4, CPA2, IDUA, BCKDHA, and CTRL. We hypothesized that HSF4 might exert its oncogenic effects in CRC via some of these genes. © 2017 IUBMB Life, 69(12):956–961, 2017
The toxicological effects of some avermectins on goat liver carbonic anhydrase enzyme
Authors: Caglayan Cuneyt , Gulcin İlhami 
Published: November 2017

AbstractAvermectins are used worldwide as antiparasitic drugs in the field of veterinary medicine and as agricultural pesticides and insecticides. Carbonic anhydrase (CA, E.C. 4.2.1.1) is a zinc‐containing metalloenzyme that catalyzes the reversible hydration of carbon dioxide (CO2) to yield protons (H+) and bicarbonate (HCO3−). In this study, some avermectins, including abamectin, doramectin, eprinomectin, and moxidectin, were investigated for in vitro inhibitory effects on the CA enzyme purified from goat liver, which was purified (125.00‐fold) using sepharose 4B‐l‐tyrosine‐sulfanilamide affinity chromatography, with a yield of 68.27% and a specific activity of 21765.31 EU/mg proteins. The inhibition results obtained from this study showed Ki values of 0.283, 0.153, 0.232, and 0.317 nM for abamectin, doramectin, eprinomectin, and moxidectin, respectively. On the other hand, acetazolamide, well‐known clinically established CA inhibitor, possessed a Ki value of 0.707 nM against goat liver CA.
New insights of subfertility among transplanted women: Immunosuppressive drug FK506 leads to calcium leak and oocyte activation before fertilization
Authors: Liu Linlin , Yang Man , Wang Naiqiang , Li Li , Chen Zi‐Jiang , Zhang Cong 
Published: November 2017

AbstractFKBP12, known as FK506 binding protein, binds to immunosuppressive drug FK506, which must be taken by patients who received organ transplant. The side effect of FK506 is that women have difficulties in bearing a baby, so it is important to find the reason of their subfertility. This research explored the expression of FKBP12 in pre‐implantation embryos and investigated its potential effect on reproduction. The results demonstrate that FKBP12 had good co‐localization with endoplasmic reticulum and inositol‐1, 4, 5‐ trisphosphate receptor in pre‐implantation stages. Inhibiting FKBP12 by FK506 significantly increased the rate of 1‐cell and fragmented embryos, greatly reduced the rate of 2‐cell embryos during in vitro fertilization. When the mice received FK506 by gavage for 21 days, the calcium intensity of oocytes was decreased, these mice were subfertile and gave birth to significantly less pups during 6‐month breeding period. QPCR demonstrated that Fbxo43 and P27kip, which are related to the release of MII oocyte arrest, and calcium channel partner protein Orai1 were downregulated, while Cdc2 and Ca2+ sensor at ER, stromal interaction molecule 1 (Stim1) were upregulated for a short time after adding FK506. Further exploration discovered that FK506 treatment increased Ca2+ concentration in the cytoplasm and caused pronucleus formation, however, the rate of parthenogenetic activation was lower compared to SrCl2 group. These findings identify the previously unknown role of FKBP12 in female reproduction which contributes to the release of calcium via IP3R channel and might open up new strategies for women who want to bear a baby after transplantation.
Examining the characteristics and clinical features of in‐ and between‐session suicide risk assessments among psychiatric outpatients
Authors: Hom Melanie A. , Stanley Ian H. , Rogers Megan L. , Sheffler Julia L. , Nelson Katherine R. , Joiner Thomas E. , Schramm Elisabeth 
Published: November 2017

AbstractObjectiveTo evaluate the characteristics of suicide risk assessments completed using the Decision Tree framework both in and between psychotherapy sessions, clinical features of patients for whom between‐session assessments are indicated, and data collected across assessments.MethodData were collected from 1,358 suicide risk assessments conducted with psychiatric outpatients (N = 41) at elevated suicide risk engaged in care at a psychology training clinic.ResultsParticipants completed an average of 4.75 (standard deviation = 4.95) suicide risk assessments (2.81 ± 4.43 between‐session assessments) per each month in treatment. Assessment frequency and patient ratings of suicidal desire and intent were each significantly associated with therapist risk level categorizations.ConclusionThe Decision Tree framework's in‐ and between‐session assessments have the potential to be implemented in a routinized format among psychiatric outpatients at elevated suicide risk. Additional research is needed to establish the feasibility and clinical utility of this framework across samples and providers.
The impact of rape acknowledgment on survivor outcomes: The moderating effects of rape myth acceptance
Authors: Wilson Laura C. , Newins Amie R. , White Susan W. 
Published: November 2017

AbstractObjectiveLittle is known about how rape acknowledgment relates to posttrauma functioning; recent research suggests the effect may depend on additional factors. In the current study, the moderating effect of rape myth acceptance (RMA) on the relationships between rape acknowledgment and mental health outcomes was examined.MethodA sample of 181 female rape survivors recruited from a university completed an online survey assessing RMA, rape acknowledgment, depression symptoms, and alcohol use.ResultsGenerally, the results supported that RMA moderated the influence of rape acknowledgment on depression symptoms and average quantity per drinking episode, but not frequency of alcohol use. The findings demonstrated that when individuals endorsed high levels of RMA, acknowledged rape survivors reported worse outcomes than unacknowledged rape survivors. Among individuals low on RMA, unacknowledged rape survivors reported worse outcomes than acknowledged rape survivors.ConclusionIt is recommended that clinicians recognize the role of survivor beliefs, such as RMA, in the relationship between labeling sexual assault experiences and mental health consequences.
Airyscan super‐resolution microscopy of mitochondrial morphology and dynamics in living tumor cells
Authors: Kolossov Vladimir L. , Sivaguru Mayandi , Huff Joseph , Luby Katherine , Kanakaraju Kaviamuthan , Gaskins H. Rex 
Published: November 2017

AbstractMitochondrial morphology is regulated by continuous fusion‐and‐fission events that are essential for maintaining normal function. Despite the prominence of mitochondrial function in energy generation and cell signaling, understanding of processes of fusion and fission dynamics has been hampered by the lack of high‐resolution optical systems that accommodate live‐cell imaging. We have examined different confocal modalities in terms of resolution and signal‐to‐noise ratio (SNR) in a point scanning confocal microscope with Airyscan super‐resolution (AS‐SR). Results indicated that Airyscan (AS) provided speed, super‐resolution, and high SNR. This modality was then used for monitoring mitochondrial dynamics in live tumor cells modified to harbor green‐fluorescent protein localized to mitochondria. We then compared regular AS and fast‐Airyscan modalities in terms of gentleness on the live‐cell samples. The fast mode provided unprecedented imaging speed that permits monitoring dynamics both in 2D and also in three‐dimensional dataset with time lapses (4D). Alterations to the mitochondrial network in U87 glioblastoma cells occurred within seconds and the cells were not affected by modest inhibition of fission. The super‐resolution permitted quantitative measurements of mitochondrial diameter with a precision that enabled detection of significant differences in mitochondrial morphology between cell lines. We have observed swelling of mitochondrial tubules in A549 lung cancer cells after 2 hr treatment with deoxynyboquinone, an ROS‐generating pharmacologic drug. We also tested different 3D analytical parameters and how they can affect morphometric quantitation. The AS‐SR imaging enabled high‐speed imaging of mitochondrial dynamics without the compromise to cell morphology or viability that is common with conventional fluorescence imaging due to photo‐oxidation.
Synthesis and Antibacterial Activity of Novel 1H‐indol‐2‐ol Derivatives
Authors: Tu Hong , Wu Shou‐Qun , Li Xiao‐Qin , Wan Zhen‐Chao , Wan Jin‐Lin , Tian Kun , Ouyang Gui‐Ping 
Published: November 2017

A series of novel 1H‐indol‐2‐ol derivatives were synthesized and evaluated their antibacterial activities against rice bacterial leaf blight, tobacco bacterial wilt, and citrus canker caused by Xanthomonas oryzae pv. oryzae (Xoo), Ralstonia solanacearum, and Xanthomonas axonopodis pv. citri via the turbidimeter test in vitro. Antibacterial bioassay indicated that most compounds demonstrated good inhibitory effect against Xoo and Ralstonia solanacearum. Especially, compound 4k demonstrated the best inhibitory effect against Xoo with half‐maximal effective concentration (EC50) value of 8.27 μg/mL, which was even better than those of commercial agents Bismerthiazol and Thiodiazole copper.
Dynamics of H2O ligands and ReO4− anions at the phase transition in [Mn(H2O)2](ReO4)2 studied by complementary spectroscopic methods
Authors: Hetmańczyk Joanna , Hetmańczyk Łukasz 
Published: November 2017

AbstractThe vibrational and reorientational motions of H2O ligands and
anions in high‐ and low‐temperature phases were investigated by means of Fourier transform far‐ and mid‐infrared and Raman light‐scattering spectroscopy and neutron scattering (inelastic/quasi‐elastic incoherent neutron scattering) methods. The dynamics of H2O and
molecules in high‐ (I) and low‐temperature (II) phases was investigated by means of band shape analysis performed for Raman and IR bands. The temperature dependencies of full width at half maximum values of the Raman bands at 874 (νas(ReO)) and 943 cm−1 (νs(ReO)) exhibit changes in their behaviour in the vicinity of transformation, suggesting that the observed phase transition is connected with a change in the reorientational dynamics of
. However, anions also perform fast (τR ≈ 10−12 to 10−13 s) stochastic reorientational motions in Phase II. The estimated mean value of activation energy for
anions in the low‐temperature phase is Ea(II) = 8.1 kJmol−1. In infrared band shape analysis it was found that H2O ligands perform fast (picosecond correlation time scale) motions in both phases with a mean value of activation energies of 7.68 kJmol−1. These reorientational motions of H2O ligands do not contribute to the phase transition mechanism. Moreover, the observed phase transition was accompanied by the splitting of some bands, suggesting a reduction in crystal structure symmetry.Quasi‐elastic neutron scattering measurements furnished evidence that H2O motion in Phase I can be fairly accurately described by a simple model of 180° jumps around a twofold axis within a picosecond time scale. Additionally, infrared, Raman light‐scattering spectra were calculated using the density functional theory method for the isolated equilibrium model Mn(H2O)2(ReO4)2, and qualitative agreement with the experimental data was achieved. The theoretical vibrational spectra of the title compound were interpreted by means of potential energy distributions using the VEDA 4 program.
Effect of farming system on donkey milk composition
Authors: Valle Emanuela , Pozzo Luisa , Giribaldi Marzia , Bergero Domenico , Gennero Maria Silvia , Dezzutto Daniela , McLean Amy , Borreani Giorgio , Coppa Mauro , Cavallarin Laura 
Published: November 2017

AbstractBACKGROUNDDonkey milk is considered as a functional food for sensitive consumers, such as children who are allergic to cow milk. No information is available regarding the effect of farming systems on the quality of donkey milk. The present study aimed to evaluate the effect of the farming system and lactation stage on donkey milk with respect to gross composition, as well as fat‐soluble vitamins and fatty acids (FA).RESULTSIndividual milk samples were collected from lactating jennies (n = 53) on the six of the largest farms located in North West Italy. The performance of lactating jennies, herd characteristics and feeding strategies were recorded at each milk sampling. The gross composition of the milk, along with the fat‐soluble vitamin content, differed in accordance with the farming system. The lactation stage had limited effects on milk quality. A higher milk fat content corresponded to a higher amount of fresh herbage proportion in the diet, with the highest polyunsaturated fatty acid (PUFA), C18:1c9, C18:3n‐3, n‐3 FA, retinol and α‐tocopherol content and the lowest concentrations of the FA that are less favorable for human health in the milk of animals fed on only forage diets.CONCLUSIONExtensive farming of dairy donkeys increased the fat content and fat‐soluble vitamin concentration of milk and also altered the FA composition to a more favorable profile for human nutrition. © 2017 Society of Chemical Industry
Modified dispersive liquid‐phase microextraction based on sequential injection solidified floating organic drop combined with HPLC for the determination of phenobarbital and phenytoin
Authors: Amiri Pebdani Arezou , Dadfarnia Shayessteh , Haji Shabani Ali Mohammad , Khodadoust Saeid , Talebianpoor Mohammad Sharif 
Published: November 2017

AbstractA modified dispersive liquid phase microextraction based on sequential injection solidified floating organic drop was developed for simultaneous separation/preconcentration of trace amounts of phenobarbital and phenytoin. The important factors affecting on the extraction recovery including pH, the volume of extraction solvent, ionic strength, and the number of injections were investigated and optimized by Box–Behnken design and desirability function. Under the optimum experimental conditions, the calibration graph was linear in the concentration range of 1.0–300.0 μg/L (r2 = 0.997) for phenobarbital and 2.0–400.0 μg/L (r2 = 0.996) for phenytoin. The limit of detection and limit of quantification were 0.35 and 1.2 μg/L for phenobarbital and 0.65 and 2.2 μg/L for phenytoin, respectively. The relative standard deviation for six replicate determinations at 10 μg/L was 3.3 and 4.1% for phenobarbital and phenytoin, respectively. The developed method was successfully applied to the determination of phenobarbital and phenytoin in urine and plasma samples.
Development of a capillary electrophoresis method for the determination of the chiral purity of dextromethorphan by a dual selector system using quality by design methodology
Authors: Krait Sulaiman , Heuermann Matthias , Scriba Gerhard K. E. 
Published: November 2017

AbstractDextromethorphan is a centrally acting antitussive drug, while its enantiomer levomethorphan is an illicit drug with opioid analgesic effects. As capillary electrophoresis has been proven as an ideal technique for enantiomer analysis, the present study was conducted in order to develop a capillary electrophoresis‐based limit test for levomethorphan. The analytical target profile was defined as a method that should be able to determine levomethorphan with acceptable precision and accuracy at the 0.1 % level. From initial scouting experiments, a dual selector system consisting of sulfated β‐cyclodextrin and methyl‐α‐cyclodextrin was identified. The critical process parameters were evaluated in a fractional factorial resolution IV design followed by a central composite face‐centered design and Monte Carlo simulations for defining the design space of the method. The selected working conditions consisted of a 30/40.2 cm, 50 μm id fused‐silica capillary, 30 mM sodium phosphate buffer, pH 6.5, 16 mg/mL sulfated β‐cyclodextrin, and 14 mg/mL methyl‐α‐cyclodextrin at 20°C and 20 kV. The method was validated according to ICH guideline Q2(R1) and applied to the analysis of a capsule formulation. Furthermore, the apparent binding constants between the enantiomers and the cyclodextrins as well as complex mobilities were determined to understand the migration behavior of the analytes.
Kinetics and Mechanism of Cumene Oxidation Initiated by N‐Hydroxyphthalimide
Authors: Sapunov V. N. , Kurganova E. A. , Koshel G. N. 
Published: November 2017

ABSTRACTThis paper discusses the results of investigations on the kinetics of cumene oxidation to cumene hydroperoxide by molecular oxygen in the presence of N‐hydroxyphthalimide as a catalyst. A mathematical model has been developed on the basis of the regularities of hydroperoxide and by‐products formation. The model adequately describes changes in all reaction components in the course of the reaction. It has also been established that the role of N‐hydroxyphthalimide in the process consists in the creation of a steady‐state concentration of N‐phthalimide radicals initiating oxidation by interacting with the hydrocarbon.
A new porcine vascularized tibial bone allotransplantation model. Anatomy and surgical technique
Authors: Kotsougiani Dimitra , Willems Joost I. , Shin Alexander Y. , Friedrich Patricia F. , Hundepool Caroline A. , Bishop Allen T. 
Published: November 2017

IntroductionVascularized bone allotransplantation may provide new options for reconstruction of segmental defects if problems of long‐term immune modulation can be solved. The current literature lacks an orthotopic large animal model, limited to bone and without the confounding effects of other tissue types, permitting a multifaceted evaluation before new methods are used clinically. The purpose of this study was to develop a large animal model for vascularized bone allotransplantation.Materials and methodsEight porcine hind limbs were dissected. Length, diameter, and location of all hindlimb vessels were measured and a single nutrient vessel supplying the tibial diaphysis identified enabling its use as a vascularized bone allotransplant. Four Yucatan minipigs were divided into two pairs with a major swine leukocyte antigen mismatch. A 3.5 cm tibial segment including its nutrient pedicle was raised simultaneously from each pig and transplanted into the matched defect of the other animal. Microarterial anastomosis of the pedicle and 3‐drug immunosuppression maintained VCA viability. Bone healing and limb function were followed for 16 weeks.ResultsA consistent tibia diaphyseal nutrient artery arose from the caudal tibial artery to enter bone a mean 2.8 mm distal to the tibial tubercle with a pedicle length of 6.6 ± 3.3 mm and diameter of 1.6 ± 0.2 mm. Using this pedicle, we reconstructed a 3.5 cm tibial defect with a vascularized bone allotransplant in four animals. Immediate weightbearing as well as progressive bone healing was demonstrated.ConclusionWe have developed a vascularized tibial bone allotranplantation large‐animal model suitable for future bone‐only allotranplantation research in mini‐pigs.