bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self‐Calibrating Copper‐Based Metal Organic Frameworks for Ratiometric Electrochemical Sensing of Creatinine
Authors: Alanazi Ahmed Z. , Alhazzani Khalid , Ali Al‐Montaser Bellah H. , Darweesh Mahmoud , Ibrahim Hossieny , El‐Wekil Mohamed M 
Published: September 2024

AbstractNanostructures that can undergo redox reactions have significant potential for electrochemical analysis. They can act as signal amplifiers to determine concentrations of targets in biological samples. In this work, a rational combination of electroactive copper‐based metal‐organic frameworks (Cu‐MOFs) and ferrocene carboxylic acid (Fc) was designed for ratiometric electrochemical detection of creatinine. The synthesized Fc@Cu‐MOFs exhibited two redox signals from the Cu2+/Cu+ and Fe3+/Fe2+ systems in the skeleton of Cu‐MOFs and Fc, respectively. In the presence of chloride (Cl−), the oxidation current of Cu+ increased due to formation of solid‐state cuprous chloride (CuCl). Adding strong Cu+ chelators, e. g. creatinine, coordinated with Cu+ and caused the current output of solid‐state CuCl to decrease significantly. The anodic current of Fc did not appreciably change, serving as an internal reference signal. The ratiometric responses (ICu(I)/IFc) changed with increasing concentrations of creatinine from 0.017–130 μM with a detection limit (S/N=3) of 0.005 μM. The main advantages of Fc@Cu‐MOFs are the low LOD, high selectivity, and reliability, making it a suitable platform for determining creatinine in human serum and urine samples. The as‐fabricated sensor is a reliable approach for determining (bio) molecules that can form stable complexes with Cu+ ions.
Enhancing fire safety and thermal performance: Wood composites with bio‐based phase change materials and fire retardants for building applications
Authors: Grzybek Jakub , Nazari Meysam , Jebrane Mohamed , Terziev Nasko , Petutschnigg Alexander , Schnabel Thomas 
Published: September 2024

AbstractThis work explores the elaboration of wood particle‐based composites incorporating bio‐based phase change materials, with epoxidized linseed oil or clay as a binder. Fire performance evaluation of the novel composites includes an assessment to determine the impact of the addition of boric acid as a fire retardant, as well as the incorporation of recycled paper fibres containing boric acid, and the application of trimethoxymethylsilane coating. The study employs thermogravimetric analysis and cone calorimetry under uniform external irradiance, with a T‐history method to analyse thermal behaviour. Results indicated that fire retardants do not compromise the energy functionality of bio‐based phase change material composites, exhibiting a latent heat of approximately 50 J/g. The density ranges from 750 to 875 kg/m3. The use of clay as a binder improves fire performance, leading to a 60% decrease in total heat release and 52% of the composite mass remaining after analysis. Although enhancing fire performance presents challenges, incorporating wood particles in clay demonstrates a promising potential approach for safe use in building applications, contributing to energy efficiency in indoor heating and cooling. The findings contribute valuable insights into these materials for creating safer and more efficient building solutions, particularly in terms of thermal regulation and fire safety.
Effect of varying dietary fatty acid patterns on early‐life food allergy in rats
Authors: Liu Manman , Chen Cheng , Wang Junjuan , Han Shiwen , Duan Sufang , Li Fang , Man‐Yau Szeto Ignatius , Liu Yali , Che Huilian , Liu Changqi 
Published: September 2024

AbstractThe prevalence of food allergies is increasing worldwide, affecting approximately 8% of children. Food allergies that develop early in life can persist throughout an individual's life. Dietary patterns, particularly those involving fatty acids (FAs), play an important role in the regulation of immune cells, thereby affecting the development of food allergies. Aimed to investigate the effects of different FA patterns on food allergies, this study established a sensitised infant rat model and fed it with feeds containing different types of FAs. We then assessed the clinical allergy symptoms, immune balance, and gut microbiota. Our animal allergy model revealed that diets rich in specific FAs exerted different regulatory effects on food allergies. Notably, n‐3 long‐chain polyunsaturated FAs exhibited the strongest inhibitory effect on food allergies, accompanied by a reduction in allergy symptoms, lower serum antibody levels, and modulation of T cell differentiation. By contrast, high levels of medium‐chain FAs promoted the occurrence and progression of food allergies. In addition, various dietary FA patterns have varying impacts on the gut microbiota, influencing overall diversity, microbial composition, and function. N‐3 long‐chain polyunsaturated FAs may be associated with a significant increase in the copy number of 15‐cis‐phytoene synthase in the intestinal flora. These findings suggest that dietary intake of different FAs during early life can affect an individual's susceptibility to food allergies by shaping the gut microbiota, which may offer a novel therapeutic approach for the treatment of food allergies.
Do longer job hours matter for maternal mental health? A longitudinal analysis of single versus partnered mothers
Authors: Simpson Julija , Wildman John , Bambra Clare , Brown Heather 
Published: September 2024

AbstractOver the past decade, single mothers have experienced increasing work requirements both in the UK and in other developed countries. Our aim was to examine if increasing job hours are associated with mental health of single mothers compared to partnered mothers. Using 13 waves of the Understanding Society Survey (2009–2023), we estimated the relationship between changing job hours and mental health using difference‐in‐difference event study design, accounting for differential treatment effects across time and individuals. We also investigated the role of potential mechanisms, including role strain and additional income. Our findings suggest that increasing job hours from part‐time to full‐time is associated with an instantaneous decrease in mental health of 0.19 standard deviations for single mothers [95% CI: −0.37;−0.01], with no effect for partnered mothers. Further analyses suggest increased role strain for single mothers as a mechanism helping explain these differences. The negative effects of increasing job hours and increased role strain should be considered when developing future welfare policies for single mothers, to ensure that greater work requirements do not undermine the mental health of the already vulnerable population group.
COVID‐19‐induced granulomatosis with polyangiitis: A case report of a 16‐year‐old East Asian and literature review
Authors: Jiang Rong , Zhou Haibo , Wen Long , Kong Xianglong , Zhou Zhiguo 
Published: September 2024

AbstractObjectiveAntineutrophil cytoplasmic antibody (ANCA)‐associated vasculitis (AAV) is divided into granulomatosis with polyangiitis (GPA), microscopic polyangiitis, and eosinophilic GPA. It is one of the most severe and potentially fatal autoimmune inflammatory conditions. The etiology and pathology of AAV are complex and poorly understood. Since the onset of the Coronavirus Disease 2019 (COVID‐19) pandemic, numerous reports have documented GPA cases following COVID‐19, suggesting a potential link between COVID‐19 and the development of GPA. This case report discusses a 16‐year‐old East Asian boy who developed GPA with diffuse alveolar hemorrhage after contracting COVID‐19. Additionally, a literature review was conducted to gain a deeper understanding of this disorder.MethodsThe study involved a retrospective analysis of the data of a case of GPA post‐COVID‐19 infection, aiming to summarize the clinical characteristics of GPA post‐COVID‐19 infection through a search of databases (PubMed, Wanfang Data, and CNKI), supplemented by standard searches in Google Scholar, Cochrane, Scopus, and LitCovid, and to conduct a comprehensive analysis of the literature.ResultsA total of 12 cases were identified and, when combined with the present case, yielded 13 cases of GPA post‐COVID‐19 infection, comprising 5 males and 8 females with an average age of (40.6 ± 19.5) years. The interval between COVID‐19 infection and the diagnosis of GPA varied from 1 day to 3 months across all cases. Mortality was reported at 7.7% (1/13). The most common clinical manifestations included cough (69.2%) and dyspnea (46.1%). Computed tomography scans revealed ground‐glass opacities and multifocal pulmonary nodules. In all cases, positive findings for c‐ANCA and protease 3‐antibody were observed. Renal involvement was observed in more than half of the patients.
Mycobacterium tuberculosis protein Rv2652c enhances intracellular survival by inhibiting host immune responses
Authors: Li Jihong , Dou Yafeng 
Published: September 2024

AbstractBackgroundsMycobacterium tuberculosis (Mtb), the pathogen responsible for tuberculosis, secretes a multitude of proteins that modulate the host's immune response to ensure its own persistence. The region of difference (RD) genes encoding proteins play key roles in TB immunity and pathogenesis. Nevertheless, the roles of the majority of RD‐encoded proteins remain to be elucidated.ObjectsTo elucidate the role of Rv2652c located in RD13 in Mtb on bacterial growth, bacterial survival, and host immune response.MethodsWe constructed the strain MS_Rv2652c which over‐expresses Mtb RD‐encoding protein Rv2652c in M. smegmatis (MS), and compared it with the wild strain in the bacterial growth, bacterial survival, virulence of Rv2652c, and determined the effect of MS_Rv2652c on host immune response in macrophages.ResultsRv2652c protein is located at cell wall of MS_Rv2652c strain and also an integral component of the Mtb H37Rv cell wall. Rv2652c can enhance the resistance of recombinant MS to various stressors. Moreover, Rv2652c inhibits host proinflammatory responses via modulation of the NF‐κB pathway, thereby promoting Mtb survival in vitro and in vivo.ConclusionOur data suggest that cell wall protein Rv2652c plays an important role in creating a favorable environment for bacterial survival by modulating host signals and could be established as a potential TB drug target.
Ferret reproductive control: what are we supposed to do now?
Authors: Ash Louise 
Published: September 2024

Background: Ferrets (Mustello furo) are an increasingly popular pet in the UK, and with this rise in popularity comes a greater need for the general practitioner to be familiar with the basic care of this species. Reproductive control in ferrets is not as straightforward as that of dogs and cats, and the options are not clear cut; however, it is inevitable that the topic of neutering will be raised by ferret owners.Aim of the article: This article aims to provide readers with the knowledge required to enable an active conversation with the ferret owner to select the most appropriate reproductive control option for their pet.
Biological potential of Argyrolobium roseum (Camb.) Jaub & Spach mediated silver nanoparticles and their effect on the growth of wheat seeds
Authors: Muneer Arooj , Akhtar Wasim , Samad Memoona , Zafar Sadia , Fatima Iram , Abidi Syed Hussain Imam , Kalsoom Razia , Shahbaz Amir 
Published: September 2024

AbstractThe green synthesis of silver nanoparticles (AgNPs) using plant‐based derivatives is getting attention for biological applications because of their small dimensions and shape. In this study, AgNPs were prepared using leaf extract of Argyrolobium roseum (A. roseum) (Camb.) Jaub. & Spach. and then characterized via Fourier transform infrared spectroscopy (FTIR), x‐ray diffraction (XRD), scanning electron microscopy (SEM) and ultraviolet–visible absorption (UV–Vis) spectroscopy. The UV‐visible spectrum displayed a absorption peak at 450 nm and x‐ray diffraction depicted a crystalline nature of biogenic NPs. FTIR analysis showed various functional groups involved in the reduction and capping of AgNPs while SEM revealed the spherical form of synthesized AgNPs. The antibacterial assay was conducted using disc diffusion assay and highest inhibition zones were recorded against Bacillus subtilis (B. subtilis) (9.6 ± 0.5 mm) and Staphylococcus aureus (S. aureus) (8.6 ± 0.5 mm). The antioxidant potential was assessed via DPPH scavenging assay and highest percentage inhibition (89%) was observed at 100 μg/mL. Subsequently, different concentrations of A. roseum AgNPs were applied on the wheat seedlings to investigate its effects on different growth parameters. After applying AgNPs, significant increase in the fresh weight (FW), dry weight (DW), root length (RL), shoot length (SL), leaf number (LN) and chlorophyll content (CC) in wheat (Akbar‐2019 variety) seedlings was observed in comparison to the control seedlings. Overall, A. roseum mediated synthesis of AgNPs was cost‐effective and safe and can be used in agriculture, biomedical and other fields.Research Highlights
Synthesis and characterization of A. roseum AgNPs was done.
Biogenic AgNPs revealed potent antibacterial and antioxidant potential.
A. roseum mediated AgNPs also increases the growth and germination of wheat seedlings.
Improved protocol for histological and histopathological preparation of large eyes
Authors: Zhang Mingliang , Lima e Silva Raquel , Zhou Lingli , Hackett Sean F. , Campochiaro Peter A. , Shen Jikui 
Published: September 2024

AbstractThe development of new treatments for ocular diseases often requires investigating eyes similar in size and structure to human eyes. Such studies are challenging because analyzing the histopathology of large, human‐sized eyes can be technically difficult. In particular, obtaining high‐quality frozen sections is almost impossible due to the formation of ice crystals in the vitreous, which causes crush artifacts during the procedures of section and post sectioning manipulations. Herein, we describe a new method that provides high‐quality frozen sections for large eyes and demonstrate its usefulness in the eyes of rabbits, pigs, minipigs, monkeys, and humans. We observed that artifactual separation of the photoreceptors from the retinal pigment epithelium is minimized and photoreceptor morphology is preserved. This method can be highly beneficial for investigators seeking to translate new treatments for ocular disease into the clinic.Research Highlights
Histopathological analysis of large and human‐sized eyes presents significant challenges, particularly in obtaining high‐quality frozen sections.
A multistep fixation followed by vitreous removal and replacement ensures better cryopreservation and embedding of large eyes, minimizing the morphological and structural retinal loss found in many studies.
Our results demonstrate that a multistep fixation and cryopreservation method for large eyes in histopathology consistently minimizes the artifactual separation of photoreceptors from the retinal pigment epithelium, thereby preserving photoreceptor morphology and providing high‐quality frozen sections.
A new method providing high‐quality sections is necessary and will be highly useful for investigators aiming to translate new treatments for ocular diseases into clinical applications.
Initial Experience of Metabolic Imaging With Hyperpolarized [1‐13C]pyruvate MRI in Kidney Transplant Patients
Authors: Liu Xiaoxi , Lai Ying‐Chieh , Cui Di , Kung Shiang‐Cheng , Park Meyeon , Laszik Zoltan , Larson Peder E. Z. , Wang Zhen J. 
Published: September 2024

BackgroundKidney transplant is the treatment of choice for patients with end‐stage renal disease. Early detection of allograft injury is important to delay or prevent irreversible damage.PurposeTo investigate the feasibility of hyperpolarized (HP) [1‐13C]pyruvate MRI for assessing kidney allograft metabolism.Study TypeProspective.SubjectsSix participants (mean age, 45.2 ± 12.4 years, two females) scheduled for kidney allograft biopsy and five patients (mean age, 59.6 ± 10.4 years, two females) with renal cell carcinoma (RCC).Field Strength/SequenceThree Tesla, T2‐weighted fast spin echo, multi‐echo gradient echo, single shot diffusion‐weighted echo‐planar imaging, and time‐resolved HP 13C metabolite‐selective imaging.AssessmentFive of the six kidney allograft participants underwent biopsy after MRI. Estimated glomerular filtration rate (eGFR) and urine protein‐to‐creatine ratio (uPCR) were collected within 4 weeks of MRI. Kidney metabolism was quantified from HP [1‐13C]pyruvate MRI using the lactate‐to‐pyruvate ratio in allograft kidneys and non‐tumor bearing kidneys from RCC patients.Statistical TestsDescriptive statistics (mean ± SD).ResultsBiopsy was performed a mean of 9 days (range 5–19 days) after HP [1‐13C]pyruvate MRI. Three biopsies were normal, one showed low‐grade fibrosis and one showed moderate microvascular inflammation. All had stable functioning allografts with eGFR >60 mL/min/1.73 m2 and normal uPCR. One participant who did not undergo biopsy had reduced eGFR of 49 mL/min/1.73 m2 and elevated uPCR. The mean lactate‐to‐pyruvate ratio was 0.373 in participants with normal findings (N = 3) and 0.552 in participants with abnormal findings (N = 2). The lactate‐to‐pyruvate ratio was highest (0.847) in the participant with reduced eGFR and elevated uPRC. Native non‐tumor bearing kidneys had a mean lactate‐to‐pyruvate ratio of 0.309.Data ConclusionStable allografts with normal findings at biopsy showed lactate‐to‐pyruvate ratios similar to native non‐tumor bearing kidneys, whereas allografts with abnormal findings showed higher lactate‐to‐pyruvate ratios.Evidence Level2Technical EfficacyStage 2
A discrete time‐to‐event model for the meta‐analysis of full ROC curves
Authors: Stoye Ferdinand Valentin , Tschammler Claudia , Kuss Oliver , Hoyer Annika 
Published: September 2024

AbstractThe development of new statistical models for the meta‐analysis of diagnostic test accuracy studies is still an ongoing field of research, especially with respect to summary receiver operating characteristic (ROC) curves. In the recently published updated version of the “Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy”, the authors point to the challenges of this kind of meta‐analysis and propose two approaches. However, both of them come with some disadvantages, such as the nonstraightforward choice of priors in Bayesian models or the requirement of a two‐step approach where parameters are estimated for the individual studies, followed by summarizing the results. As an alternative, we propose a novel model by applying methods from time‐to‐event analysis. To this task we use the discrete proportional hazard approach to treat the different diagnostic thresholds, that provide means to estimate sensitivity and specificity and are reported by the single studies, as categorical variables in a generalized linear mixed model, using both the logit‐ and the asymmetric cloglog‐link. This leads to a model specification with threshold‐specific discrete hazards, avoiding a linear dependency between thresholds, discrete hazard, and sensitivity/specificity and thus increasing model flexibility. We compare the resulting models to approaches from the literature in a simulation study. While the estimated area under the summary ROC curve is estimated comparably well in most approaches, the results depict substantial differences in the estimated sensitivities and specificities. We also show the practical applicability of the models to data from a meta‐analysis for the screening of type 2 diabetes.
The effect of terroir on volatilome fingerprinting and qualitative attributes of non‐irrigated grapes reveals differences on glycosylated aroma compounds
Authors: Georgiadou Egli C. , Mina Minas , Valanides Nicolas , Taliadorou Anna‐Maria , Koundouras Stefanos , D'Onofrio Claudio , Bellincontro Andrea , Mencarelli Fabio , Barbayiannis Nikolaos , Fotopoulos Vasileios , Manganaris George A. 
Published: September 2024

AbstractBACKGROUND‘Xynisteri’ is considered as the reference white grape cultivar in Cyprus with remarkable adaptation to adverse edaphoclimatic conditions and appreciable oenological properties that renders it as an appropriate cultivar for studies within a global context due to climate change. To this aim, two distinct non‐irrigated plots with different climatic conditions, soil properties and levels of rainfall were selected; Koilani [KO, altitude 800 m, 76% calcium carbonate (CaCO3) content, pH 7.97, average temperature: 16.5 °C, rainfall: 229 mm] and Kyperounda (KY, altitude 1200 m, CaCO3‐free soil, pH 6.47, average temperature: 14.9 °C, rainfall: 658 mm). An array of physiological, biochemical and qualitative indices during successive developmental stages (BBCH 75–89) were determined. During the advanced on‐vine developmental stages (BBCH 85–89), the aromatic profile of grapes was assessed with the employment of gas chromatography–mass spectrometry (GC–MS). Such analysis was complemented with non‐destructive chemometric analyses.RESULTSBerry ripening process substantially differed on the examined plots; BBCH 89 stage reached at 267 and 303 Julian days for KO and KY, respectively. Results indicated that berry weight, soluble solids content (SSC) and α‐amino nitrogen were higher in KO than in KY, with exception made for ammonium nitrogen content. A total of 75 compounds, including aliphatic alcohols, benzenic compounds, phenols, vanillins, monoterpenes and C13‐norisoprenoids were identified and quantified. The variations of mesoclimatic conditions affected the volatile organic compound (VOC) profiles at the fully‐ripe stage, showing a considerable rise in glycosylated aroma compounds, especially monoterpenes and benzenic compounds. In particular, the higher amount of glycosylated aroma compounds were obtained in KY berries up to mid‐ripe, whereas KO showed higher glycosylated aroma compounds at fully‐ripe stage. Results reported herein indicate that aroma profile of ‘Xynisteri’ grapes varied substantially in the examined terroirs. Interestingly, the limited rainfall in KΟ non‐irrigated vine did not compromise qualitative and aromatic properties of berries.CONCLUSIONSThe present study aimed at dissecting the impact of terroir on bush‐trained, non‐irrigated grapevines of a cultivar appropriate for extreme climate change scenarios. The volatilome fingerprint was highly variable among the examined plots; such results can be further exploited at vinification level towards production of single vineyard premium end products. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.