bluuebunny/crossref_metadata_2025_split huggingface dataset:
A review of multi-sensory technologies in a Science, Technology, Engineering, Arts and Mathematics (STEAM) classroom
Authors: Taljaard Johann 
Published: October 2016

<p class="JLDAbstract">This article reviews the literature on multi-sensory technology and, in particular, looks at answering the question: ‘What multi-sensory technologies are available to use in a science, technology, engineering, arts and mathematics (STEAM) classroom, and do they affect student engagement and learning outcomes?’ Here engagement is defined as motivation, interest, curiosity and attitude. This review identifies tools and software from a burgeoning, yet limited literature. To answer this question, this review will include the following: defining learning styles; background on multi-sensory instruction; multi-sensory education today; STEM and the rise of STEAM; a look at some multi-sensory tools; and the benefits of using multi-sensory technologies in education. </p>
De slag om duurzaamheid in de polycentrische regio’s Randstad en Rijn-Roergebied
Authors: Goess Simon , van Bueren Ellen , de Jong Martin 
Published: October 2016

The battle for sustainability in the polycentric regions Randstad and Rhine-Ruhr area
In polycentric urban regions one can find different, mutually related cities without a clear centre. In these regions cities cooperate to attract inhabitants and employment, but at the same time they are each other’s competitors. The Randstad (Netherlands) and the Rhine-Ruhr area (Germany) both can be seen as polycentric regions. The authors explore to what extent these regions possess a common identity and common agenda and to what extent this promotes the sustainability and energy transition of these regions. In both regions identity appears to have grown especially at subregional level, by historically developed spatial-economic profiles of the different cities or suburban regions. In addition the cities in these regions more and more wish to distinguish themselves in the area of sustainability. Every city wants to be the smartest, greenest and healthiest, and to be at the forefront in energy transition and climate mitigation. In the Dutch Randstad this competitive drive especially seems to contribute to the realization of sustainability projects at the local level. And that is exactly why regional cooperation is important: to allocate resources as well as possible and to avoid transfer to others. This can be improved by the development of subregional or regional sustainability visions.
Polycentrisme in lokale besluitvorming over duurzame energie: de casus slimme netten
Authors: Lammers Imke , Arentsen Maarten 
Published: October 2016

Polycentrism in local decision-making on sustainable energy: the case of smart grids
Energy planning and the realization of a new energetic infrastructure has become an issue for many actors. The local setting has become polycentric. Against this background the authors have tried to answer the question of the possible consequences of a polycentric local decision-making arena for the realization of sustainable energy transition, especially the implementation of smart grids. Polycentrism is characterised by configurations of units that are multi-level, multi-purpose, multi-sectoral and multi-functional. The impact of these configurations can be assessed using four criteria: control, efficiency, political representation and local self-determination. The authors used these criteria to analyse two cases. Both cases show that the consequences of polycentrism are variable and differ on the four criteria. The analysis shows tensions in polycentric configurations between control and efficiency on the one hand and local self-determination and political representation on the other. This outcome was a reason for the authors to argue for a better institutional design for the local polycentric arena with the help of the seven ‘rules-in-use’ of Elinor Ostrom. Her design is universal but requires specific local application. In this way more justice can be done to the local circumstances in order to be able to achieve effective results.
Energiecoöperaties en gemeenten: bondgenoten bij lokale energietransitie?
Authors: Hufen Hans 
Published: October 2016

Energy cooperatives and municipalities: allies in local energy transition?
Do energy cooperatives work together with municipalities in the area of energy and, if this is so, how can this cooperative relationship be interpreted from a public administration perspective? That is the central question of this article. The experiences with cooperation of four frontrunners amongst the energy cooperatives show that in many areas a fruitful cooperative relationship has been developed. In other areas cooperation is lacking because the municipality stands aloof as soon as the energy cooperative provides services to citizens and/or companies or because the purchase of green energy by the municipality from their own energy cooperative cannot simply be carried out. In the development of renewable energy projects it also suits municipalities to be reluctant because they not only promote local renewable energy but are also responsible for the spatial quality. From the perspective of public administration it is striking that the variety of municipal roles increases the complexity of cooperative relationship with energy cooperatives. For energy cooperatives it is difficult to understand that the municipality sometimes behaves like an ally, but can also be reluctant. The variety of the bond between both parties is first of all apparent from the need of an own identity and autonomy in the energy cooperatives. Secondly, two of the four energy cooperatives that were analysed needed support in a financial emergency.
Dansk Industri: nadere afbakening grenzen aan richtlijnconforme interpretatie en horizontale werking algemeen Unierechtelijk beginsel?
Authors: Haket S.W. 
Published: October 2016

Dansk Industri: nadere afbakening grenzen aan richtlijnconforme interpretatie en horizontale werking algemeen Unierechtelijk beginsel?
In het arrest Dansk Industri oordeelt het Hof van Justitie dat het de nationale rechter niet is toegestaan enkel op basis van vaste rechtspraak de mogelijkheid van een richtlijnconforme interpretatie van een nationale bepaling af te wijzen. Indien de nationale bepaling desondanks niet richtlijnconform kan worden geïnterpreteerd, dient zij buiten toepassing te worden gelaten voor zover zij onverenigbaar is met het algemene verbod van discriminatie op grond van leeftijd. Het rechtszekerheidsbeginsel doet hier niet aan af.HvJ 19 april 2016, zaak C-441/14, Dansk Industri (DI)/Nalatenschap van Karsten Eigil Rasmussen, ECLI:EU:C:2016:278
Macht aus dem Rechtsstaat keinen Gurkensalat
Authors: van Gestel R.A.J. , van Lochem P.J.P.M. 
Published: October 2016

Macht aus dem Rechtsstaat keinen Gurkensalat
Deze bijdrage is een reactie op het artikel ‘De rechtsstaat: van sluitpost naar “Leitmotiv”’ van Zouridis, Wierenga en Niemeijer in het Nederlands Juristenblad, waarin kritiek wordt geleverd op het eerste hoofdstuk van het jaarverslag van de Raad van State. De Raad waarschuwt daarin voor het zien van rechtsstatelijke overwegingen in de politiek als een soort van sluitpost. Zouridis, Wierenga en Niemeijer vragen zich af op basis van welke empirische feiten de conclusies van de Raad van State zijn gebaseerd en of deze wel de juiste oorzaken voor het afkalven van de rechtsstaat in het vizier heeft. De auteurs van deze reactie vragen zich op hun beurt echter af of het betoog in dat artikel op zijn beurt wel op voldoende empirische onderbouwing steunt. Het gaat de auteurs daarbij met name om de onderbouwing van de stelling dat bestuur en wetgever ‘regelverslaafd’ zijn, de vraag wat het aantal wettelijke regels zegt over het rechtsstatelijke gehalte van de samenleving en welke rol overheidsjuristen vervullen bij het bewaken van rechtsstatelijke normen. Aan het slot van de bijdrage gaan de auteurs in op de vraag of er niet sprake is van een bredere trend, die twijfels oproept over de wijze waarop binnen het rechtswetenschappelijk onderzoek met empirische data en bronnenonderzoek wordt omgegaan.
El error neurocientífico de Descartes, entre Spinoza y Aquinas. El debate entre Damasio y Stump sobre el carácter eliminativo o vitalista del materialismo en la neuroética, neuropolítica y neuroeconomía
Authors: Landázuri Carlos Ortiz de 
Published: October 2016

Se analiza el debate entre Eleonore Stump y Antonio Damasio respecto de dos posibles modelos de autorregulación que hoy día se asignan a la neuroética, neuropolítica y neuroeconomía a la hora de correlacionar la mente y el cerebro, a saber: o bien se sigue el modelo híbrido de tipo monista que utilizó Spinoza, siguiendo a su vez la interpretación materialista eliminativa de Antonio Damasio, para de este modo lograr corregir el «error» neurocientífico de Descartes, ya previamente denunciado por Popper y Eccles; o bien se recurre a un modelo dualista de integración vital entre la mente y el cerebro, como el de Tomás de Aquino y aún antes Aristóteles, siguiendo a su vez la interpretación jerarquizada, interactiva y deliberativa de Eleonore Stump. Además, también se pretende mostrar la superioridad de este segundo materialismo vitalista sobre aquel otro meramente eliminativo a la hora de correlacionar mente y cerebro con ayuda de estos tres saberes.It is analysed the debate between Eleonore Stump and Antonio Damasio concerning two possible models of auto-regulation that today are assigned to the neuro-ethic, neuropolitic and neuro-economy when correlating the mind and the brain, with two possibilities: Or it follows the hybrid model of monist type that used Spinoza, following at the same time the Damasio’s eliminative materialist interpretation, for in this way attaining to correct the Descartes’s neuro-scientific «error»; or it is resorted to a dualist model of vital integration between the mind and the brain, as the one of Aquinas and still before Aristotle, following at the same time the Stump’s hierarchical, interactive and deliberative interpretation. Besides, it also attempts to show the superiority of this second vital materialism on that another merely eliminative when correlating relate mind and brain with the help of these three sciences.
A novel, all-dielectric, microwave plasma generator towards development of plasma metamaterials
Authors: Cohick Zane , Luo Wei , Perini Steven , Baker Amanda , Wolfe Douglas , Lanagan Michael 
Published: October 2016

Abstract
A proof of concept for a microwave microplasma generator that consists of a halved dielectric resonator is presented. The generator functions via leaking electric fields of the resonant modes — TE01δ and HEM12δ modes are explored. Computational results illustrate the electric fields, whereas the stability of resonance and coupling are studied experimentally. Finally, a working device is presented. This generator promises potentially wireless and low-loss operation. This device may find relevance in plasma metamaterials; each resonator may generate the plasma structures necessary to manipulate electromagnetic radiation. In particular, the all-dielectric nature of the generator will allow low-loss interaction with high-frequency (GHz–THz) waves.
ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules
Authors: Luo Youguang , Ran Jie , Xie Songbo , Yang Yunfan , Chen Jie , Li Shanshan , Shui Wenqing , Li Dengwen , Liu Min , Zhou Jun 
Published: October 2016

AbstractOrientation and positioning of the mitotic spindle are involved in dictating cell division axis and cleavage site, and play important roles in cell fate determination and tissue morphogenesis. However, how spindle movement is controlled to achieve a defined alignment within the dividing cell is not fully understood. Here, we describe an unexpected role for apoptosis signal-regulating kinase 1 (ASK1) in regulating spindle behavior. We find that ASK1 is required for proper mitotic progression and daughter cell adhesion to the substratum. ASK1 interacts with end-binding protein 1 (EB1) and phosphorylates EB1 at serine 40, threonine 154 and threonine 206, enhancing its binding to the plus ends of astral microtubules. Consequently, astral microtubules are stabilized and therefore capable of mediating spindle interaction with the cell cortex, a requirement for spindle movement. These findings reveal a previously undiscovered function of ASK1 in cell division by regulating spindle orientation and positioning, and point to the importance of protein phosphorylation in the regulation of spindle behavior.
Oscillating edge states in one-dimensional MoS2 nanowires
Authors: Xu Hai , Liu Shuanglong , Ding Zijing , Tan Sherman J. R. , Yam Kah Meng , Bao Yang , Nai Chang Tai , Ng Man-Fai , Lu Jiong , Zhang Chun , Loh Kian Ping 
Published: October 2016

AbstractReducing the dimensionality of transition metal dichalcogenides to one dimension opens it to structural and electronic modulation related to charge density wave and quantum correlation effects arising from edge states. The greater flexibility of a molecular scale nanowire allows a strain-imposing substrate to exert structural and electronic modulation on it, leading to an interplay between the curvature-induced influences and intrinsic ground-state topology. Herein, the templated growth of MoS2 nanowire arrays consisting of the smallest stoichiometric MoS2 building blocks is investigated using scanning tunnelling microscopy and non-contact atomic force microscopy. Our results show that lattice strain imposed on a nanowire causes the energy of the edge states to oscillate periodically along its length in phase with the period of the substrate topographical modulation. This periodic oscillation vanishes when individual MoS2 nanowires join to form a wider nanoribbon, revealing that the strain-induced modulation depends on in-plane rigidity, which increases with system size.
Global cooling as a driver of diversification in a major marine clade
Authors: Davis Katie E. , Hill Jon , Astrop Tim I. , Wills Matthew A. 
Published: October 2016

AbstractClimate is a strong driver of global diversity and will become increasingly important as human influences drive temperature changes at unprecedented rates. Here we investigate diversification and speciation trends within a diverse group of aquatic crustaceans, the Anomura. We use a phylogenetic framework to demonstrate that speciation rate is correlated with global cooling across the entire tree, in contrast to previous studies. Additionally, we find that marine clades continue to show evidence of increased speciation rates with cooler global temperatures, while the single freshwater clade shows the opposite trend with speciation rates positively correlated to global warming. Our findings suggest that both global cooling and warming lead to diversification and that habitat plays a role in the responses of species to climate change. These results have important implications for our understanding of how extant biota respond to ongoing climate change and are of particular importance for conservation planning of marine ecosystems.
Multicolour synthesis in lanthanide-doped nanocrystals through cation exchange in water
Authors: Han Sanyang , Qin Xian , An Zhongfu , Zhu Yihan , Liang Liangliang , Han Yu , Huang Wei , Liu Xiaogang 
Published: October 2016

AbstractMeeting the high demand for lanthanide-doped luminescent nanocrystals across a broad range of fields hinges upon the development of a robust synthetic protocol that provides rapid, just-in-time nanocrystal preparation. However, to date, almost all lanthanide-doped luminescent nanomaterials have relied on direct synthesis requiring stringent controls over crystal nucleation and growth at elevated temperatures. Here we demonstrate the use of a cation exchange strategy for expeditiously accessing large classes of such nanocrystals. By combining the process of cation exchange with energy migration, the luminescence properties of the nanocrystals can be easily tuned while preserving the size, morphology and crystal phase of the initial nanocrystal template. This post-synthesis strategy enables us to achieve upconversion luminescence in Ce3+ and Mn2+-activated hexagonal-phased nanocrystals, opening a gateway towards applications ranging from chemical sensing to anti-counterfeiting.
Repeated superovulation may affect mitochondrial functions of cumulus cells in mice
Authors: Xie Juan-Ke , Wang Qian , Zhang Ting-Ting , Yin Shen , Zhang Cui-Lian , Ge Zhao-Jia 
Published: October 2016

AbstractControlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function.
Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
Authors: Wang Jinzi , Shi Liming , He Xipu , Lu Lidan , Li Xiaoping , Chen Baoshan 
Published: October 2016

AbstractTo understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identify and quantify the secreted proteins. A total of 403 unique proteins were identified from the secretome of the wild type virus-free strain EP155. Of these proteins, 329 were predicted to be involved in known secretory pathways and they are primarily composed of metabolic enzymes, biological regulators, responders to stimulus and components involved in plant-pathogen interactions. When infected with the hypovirus CHV1-EP713, 99 proteins were found to be differentially expressed as compared to the wild type strain EP155. These proteins were mainly related to plant cell wall degradation, response to host defense, fungal virulence and intracellular structure. The effects of CHV1 on secreted proteins may reveal a relationship between physiological pathways and hypovirulence.
Comparing performance of modern genotype imputation methods in different ethnicities
Authors: Roshyara Nab Raj , Horn Katrin , Kirsten Holger , Ahnert Peter , Scholz Markus 
Published: October 2016

AbstractA variety of modern software packages are available for genotype imputation relying on advanced concepts such as pre-phasing of the target dataset or utilization of admixed reference panels. In this study, we performed a comprehensive evaluation of the accuracy of modern imputation methods on the basis of the publicly available POPRES samples. Good quality genotypes were masked and re-imputed by different imputation frameworks: namely MaCH, IMPUTE2, MaCH-Minimac, SHAPEIT-IMPUTE2 and MaCH-Admix. Results were compared to evaluate the relative merit of pre-phasing and the usage of admixed references. We showed that the pre-phasing framework SHAPEIT-IMPUTE2 can overestimate the certainty of genotype distributions resulting in the lowest percentage of correctly imputed genotypes in our case. MaCH-Minimac performed better than SHAPEIT-IMPUTE2. Pre-phasing always reduced imputation accuracy. IMPUTE2 and MaCH-Admix, both relying on admixed-reference panels, showed comparable results. MaCH showed superior results if well-matched references were available (Nei’sGST ≤ 0.010). For small to medium datasets, frameworks using genetically closest reference panel are recommended if the genetic distance between target and reference data set is small. Our results are valid for small to medium data sets. As shown on a larger data set of population based German samples, the disadvantage of pre-phasing decreases for larger sample sizes.
Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli
Authors: Douzi Badreddine , Brunet Yannick R. , Spinelli Silvia , Lensi Valentine , Legrand Pierre , Blangy Stéphanie , Kumar Anant , Journet Laure , Cascales Eric , Cambillau Christian 
Published: October 2016

AbstractThe Type VI secretion system (T6SS) is a versatile machine that delivers toxins into either eukaryotic or bacterial cells. It thus represents a key player in bacterial pathogenesis and inter-bacterial competition. Schematically, the T6SS can be viewed as a contractile tail structure anchored to the cell envelope. The contraction of the tail sheath propels the inner tube loaded with effectors towards the target cell. The components of the contracted tail sheath are then recycled by the ClpV AAA+ ATPase for a new cycle of tail elongation. The T6SS is widespread in Gram-negative bacteria and most of their genomes carry several copies of T6SS gene clusters, which might be activated in different conditions. Here, we show that the ClpV ATPases encoded within the two T6SS gene clusters of enteroaggregative Escherichia coli are not interchangeable and specifically participate to the activity of their cognate T6SS. Here we show that this specificity is dictated by interaction between the ClpV N-terminal domains and the N-terminal helices of their cognate TssC1 proteins. We also present the crystal structure of the ClpV1 N-terminal domain, alone or in complex with the TssC1 N-terminal peptide, highlighting the commonalities and diversities in the recruitment of ClpV to contracted sheaths.
Deciphering KRAS and NRAS mutated clone dynamics in MLL-AF4 paediatric leukaemia by ultra deep sequencing analysis
Authors: Trentin Luca , Bresolin Silvia , Giarin Emanuela , Bardini Michela , Serafin Valentina , Accordi Benedetta , Fais Franco , Tenca Claudya , De Lorenzo Paola , Valsecchi Maria Grazia , Cazzaniga Giovanni , Kronnie Geertruy te , Basso Giuseppe 
Published: October 2016

AbstractTo induce and sustain the leukaemogenic process, MLL-AF4+ leukaemia seems to require very few genetic alterations in addition to the fusion gene itself. Studies of infant and paediatric patients with MLL-AF4+ B cell precursor acute lymphoblastic leukaemia (BCP-ALL) have reported mutations in KRAS and NRAS with incidences ranging from 25 to 50%. Whereas previous studies employed Sanger sequencing, here we used next generation amplicon deep sequencing for in depth evaluation of RAS mutations in 36 paediatric patients at diagnosis of MLL-AF4+ leukaemia. RAS mutations including those in small sub-clones were detected in 63.9% of patients. Furthermore, the mutational analysis of 17 paired samples at diagnosis and relapse revealed complex RAS clone dynamics and showed that the mutated clones present at relapse were almost all originated from clones that were already detectable at diagnosis and survived to the initial therapy. Finally, we showed that mutated patients were indeed characterized by a RAS related signature at both transcriptional and protein levels and that the targeting of the RAS pathway could be of beneficial for treatment of MLL-AF4+ BCP-ALL clones carrying somatic RAS mutations.