bluuebunny/crossref_metadata_2025_split huggingface dataset:
The production and analysis of carotenoid preparations from some strains of xylotrophic Basidiomycetes
Authors: Velygodska A. K. , Fedotov O. V. 
Published: November 2016

The aim of the study was selection of optimal conditions for obtaining carotenoid drugs of mycelium origin from the basidiomycete strains Laetiporus sulphureus Ls-08, Fomes fomentarius Ff-1201 and Fistulina hepatica Fh-18 and the study of antibacterial and total antioxidant activity of these compounds. The strains were surface grown on a glucose-peptone medium modified for each producer. The homogenized pigments of the mycelium strains were extracted with ethanol and the solvent was separated under vacuum at 60 ºC. The absorption spectra of the carotenoid drugs were recorded for alcoholic solutions at 350–500 nm. The antibacterial activity of the carotenoids was determined by the agar diffusion method, and the total antioxidant activity was determined by the DPPH-method. It was found that the optimum temperature for carotenoid extraction is 60 °C. The absorption spectra of carotenoid drugs showed three peaks in 420, 450 and 470 nm. These results respond to the β-carotene absorption spectra. The highest antioxidant activity was noted for carotenoid drugs from F. hepatica Fh-18 and L. sulphureus Ls-08 strains obtained at an extraction temperature of 40 and 60 °C respectively. The antibacterial activity of carotenoid drugs against the test cultures was not species dependent. Carotenoid drugs with a 20% concentrate obtained from the L. sulphureus Ls-08 strain had the highest antibacterial activity against the test cultures Staphylococcus aureus and Escherichia coli. Carotenoids from the mycelium of F. hepatica Fh-18 had the highest antibacterial activity against the test culture Candida albicans. Extraction temperature of 60 °C is optimal for mycelial yield of carotenoids from the studied strains. All preparations of carotenoids exhibited antibacterial activity against the test microorganism cultures. The carotenoid drugs obtained at 40 and 60 °C from the strains F. hepatica Fh-18 and L. sulphureus Ls-08 respectively showed the highest antioxidant activity.
Nesting of Great Cormorants (Phalacrocorax carbo) on man-made structures in Ukraine
Authors: Sidorenko A. I. , Siokhin V. D. 
Published: November 2016

In Ukraine the Great Cormorant (Phalacrocorax carbo Linnaeus, 1758) uses a rather wide range of habitats for nesting: islands, trees and shrubs, reedbeds and a variety of man-made structures. In general, the strategy of nesting on man-made structures is uncommon both in Ukraine and Europe, and Cormorantsdo this only in the absence of other sites suitable for nesting. Special research onCormorant colonies on technogenic constructions was carried out during the field expeditions by the Research Institute of Biodiversity of Terrestrial and Aquatic Ecosystems of Ukraine in 2002–2003 and 2012–2016. Besides this, we used retrospective and current data from the literature and Internet resources. Most of the field work was carried out by making surveys by boat and on foot. As a result, we found 8 Cormorant colonies on technogenic constructions in Ukraine: gas platforms in the Sea of Azov (near the village Strilkove, Henichesk district of Kherson region); sunken ships – targets for bombing training near the Arabat Spit (these are also known as «ship islands»); electricity pylons of the high-voltage Enerhodar Dnipro Power Line where it crosses the Kakhovka Reservoir; the dock in Yahorlyk Bayk, used in the past as a target for bombing training bombing; artificial island-platforms on Lake Chernine (Kinburn Peninsula); an artificial island on the Sasyk Lagoon (Odessa region); artificial islands, made as navigation markers on the Kremenchuk and Kiev reservoirs. The study found that in most cases the accompanying species was the CaspianGull (Larus cachinnans Pallas, 1811), which actively destroys the Cormorants’ nests and eats their eggs and chicks. The number of nests in the colonies varied greatly (5–30 nests on the navigation marker islands and ca. 2 000–2 300 on the «ship-islands» and gas platforms). This is due, primarily, to the area of the breeding territory. The research found that fierce territorial competition was observed in most of the colonies both with Caspian Gulls and between Cormorants. In addition, we observed anthropogenic interference in the colonies by fishermen and workers conducting routine maintenance work (as happened in the case of electricity pylons and gas platforms). The benefit of this study is that it is the first research in Ukraine conducted at national level onthis type of nesting by Cormorants. Moreover, the study examines the history of emergence of these nesting territories and population dynamics of the Great Cormorant from the time of initial settlement of the breeding sites till the present.
Utilization of fumarate by sulfur-reducing bacteria Desulfuromonas sp.
Authors: Сhayka O. , Peretjatko T. , Gudz S. , Halushka A. 
Published: November 2016

The main goal of the work was to study the utilization of fumarate by sulfur-reducing bacteria Desulfuromonas sp. under different growth conditions and accumulation of hydrogen sulfide by bacteria in the media with sulfur and different electron donors. Sulfur-reducing bacteria Desulfuromonas sp., isolated from soil in Yazivske sulfur deposit, were used in the reasearch. Bacteria were grown in the medium Postgate C without sulfates. The content of hydrogen sulfide was determined by formation of methylene blue. The content of organic acids (fumarate, succinate, lactate, acetate) was determined by high performance liquid chromatography (HPLC). The biomass of cells was determined by the photoelectrocolorymetry method using KFK-3. The highest level of accumulation of hydrogen sulfide by bacteria Desulfuromonas sp. was found in media with sodium lactate and sodium pyruvate. The maximal concentration of hydrogen sulfide was 1.9 mM. Maximal accumulation of biomass was observed in the media with malate, lactate and fumarate with the presence of elemental sulfur. Sulfur-reducing bacteria Desulfuromonas sp. are able to utilize fumarate as an electron donor and acceptor in the absence of elemental sulfur in the medium. After the incubation of Desulfuromonas sp. in the medium with fumarate, chromatographic analysis of culture liquid showed that fumarate is converted to succinate and small quantities of acetate The presence of acetate is, probably, due to the particularaties of the functioning of citric acid cycle in bacteria of the genus Desulfuromonas. Consequently, the results indicate that the fumarate serves as a donor and acceptor of electrons.The simultaneous introduction of two electron donors – fumarate and elemental sulfur – was accompanied by inhibition of sulfur reduction. After an additional source of carbon (sodium lactate) and electron acceptor (elemental sulfur) was added to the medium with fumarate a fivefold increase of sulfidogenic activity was observed. Thus, regulation of respiration in bacteria Desulfuromonas sp. is directed to the primary utilization of the most energetically favorable electron acceptors.
Analysis of the parameters of the assimilation component of aboveground biomass of forest-forming species in the steppe zone of Ukraine
Authors: Sytnyk S. A. , Lovynska V. M. , Gritsan Y. I. , Maslikova K. P. 
Published: November 2016

The purpose this research is to study the parameters of leaf (needle) share in the trees’ greenery fraction and the content of absolutely dry matter in fresh leaves of black locust and Scots pine. The leaf (needle) share in the trees greenery fraction and the content of absolutely dry matter were determined by their quantitative measures (weight and volume). The results of the research reveal that the leaf share in the structure of a tree’s greenery fraction has a broad range of values: 43.0–72.8% for black locust and 49.1–75.4% for Scots pine. The minimum value of this parameter was recorded for an overmature Robinia specimen of 41 years of age, while the maximum was for a 3-year-old tree. For pine trees the lowest values of the given parameter were registered for the spcimens aged 38, 49 and 84, the maximum – for 30–31-year-old trees. For both investigated species it should be noted that there is a consistent pattern indicated by the following trend line: with the increase of tree age, height and trunk diameter, there is a decrease of leaf share value in the trees’ greenery fraction. Such characteristic parameter as absolutely dry mass has a sufficient range of values from 0.321 to 0.524, with the extreme values for the trees belonging to the young stock group in the case of the black locust. The absolutely dry matter content in Scots pine needles showed a significant variability of values from 0.426 to 0.620. The trend line shows a tendency of increase in the value of absolutely dry matter mass in the leaves of both investigated species with the increase in the values of the tree taxation parameters. There is no statistically proven dependency of the parameter indicating leaf share in the trees greenery fraction on the age, trunk diameter and height of trees. The most important biometric indicator, which shows a moderate relationship with the greenery fraction of a tree is the average diameter of the trunk of model trees of the two studied species. This is confirmed by values of correlation coefficients. The indicator of greenery fraction is inversely dependent on the height, trunk diameter and tree age, i.e. the increase in the values of these parameters leads to the decrease in the share of the photosynthetic active component of trees of the studied tree species in the steppe zone. The value of leaf (needle) share in trees’ greenery fraction decreases with the increasing age, height and diameter values, which is quite natural. Correlation indices of absolutely dry matter according to age, height and diameter of sample trees have negative values, while the index of leaf (needle) share of trees’ greenery fraction has a direct correlation with all the studied influence factors.
Long-term dynamics of hydrological and hydro-chemical indicators of Kytai Lake (Odessa region, Ukraine)
Authors: Dzhurtubaev M. M. , Urbanskaya T. V. , Dzhurtubaev Y. M. 
Published: November 2016

Odessa region contains a unique natural complex – the Danube lakes. One of the largest is Kytai Lake. After their construction in the second half of the XX century, dams have drastically changed the ecological situation in the lakes, including hydrological and hydro-chemical parameters. 120 water samples were taken from the lake in the years 2004–2013. 1,390 measurements were taken according to 11 indicators. Our research also used historical data for 2004–2005. Studies have shown that the hydrological and hydrochemical situation in Kytai Lake deteriorated in the period 2004 to 2013. There was a marked decrease in the water transparency and a steady increase in the amount of suspended matter in the water (from 29.4 mg/dm³ in 2006 to 143.2 mg/dm³ in 2012). The salinity of the water greatly increased (up to 6200 mg/ dm³ in September – November 2012). Permanganate oxidization permanently and significantly exceeeds the maximum permissible value of BOD5. The amount of dissolved oxygen, total nitrogen, phosphorus, nitrates and pH conform to the permitted norm, although the situation with these indicators has also gradually worsened. The average annual temperature of the water was 11.4–15.8 °C, which corresponds to the temperature regime of the continental waters in the region. To improve the ecological situation in the lake, it is necessary to ensure that the pumping station supplies a stable inflow of water from the Danube, which would significantly lower the excessive salinity of the lake.
The physiological state of the larvae and infestation of domestic ungulates by Echinococcus granulosus (Cyclophyllidea, Taeniidae) in North-Eastern Ukraine
Authors: Yemets A. M. , Demenko V. M. , Kabanyets V. V. 
Published: November 2016

Echinococcus granulosus (Batsch, 1786) has a worldwide distribution. Different types of carnivores (dogs, jackals, wolves, foxes), domestic and wild ungulates are included in its life cycle. There are significant differences in its pattern of contamination of domestic ungulates in the various regions of Ukraine. Pigs are major intermediate hosts of E. granulosus in the northern and western regions of the country, sheep and cattle in the southern and eastern regions. These differences are associated with strains of Echinococcus. The article presents the results of research conducted on 1,884 pigs, 1,025 sheep and 1,406 cattle in Polesia and the forest-steppe zone of the north-east of Ukraine. We have established the development of Echinococcus larvae in pigs (extent of invasion 10.2 ± 0.6%) and sheep (extent of invasion 0.7 ± 0.2%). The cysts did not develop in cattle. Pigs generally had a low invasion intensity (less than 6 larvae). A high level of invasion intensity (more than 10 larvae) was infrequently recorded in these animals. All sheep had a low level of invasion intensity.The E. granulosus larvae found in sheep were dead. In pigs, most cysts developed to fertile state (139 cases). Young, sterile and dead larvae were less common (39, 10, 4 cases). Pigs older than 10 months had the highest number of fertile cysts. Young larvae dominated in pigs younger than 10 months. In these animals, larvae were localized predominantly in the liver. The kidneys and lungs were infected less frequently. The large number of fertile cysts in pigs older than 10 months made this group of animals the main intermediate hosts of E. granulosus in the north-eastern part of Ukraine. They enable the parasite to function actively here. Analysis of infestation by E. granulosus larvae of domestic ungulates of the Polesia and the forest-steppe zone showed no significant differences. Identified differences were not statistically confirmed.
Seasonal population dynamics of Brachyiulus jawlowskii (Diplopoda, Julidae) in the Dnieper river arena
Authors: Gudym N. G. 
Published: November 2016

We have researched the population dynamics of Brachyiulus jawlowskii Lohmander, 1928 in the arena of the Dnepr river (within the "Dnieper-Orilsky” Nature Reserve) and also present a full picture of the habitat distribution of Julidae within the researched area. The tested models were basic types of arena biogeocenosis: sand steppe, black maple forests, artificial pine plantations, deciduous forest, meadow and swamp. Variation in population density of B. jawlowskii is determined by biotopical features. The swamp and meadow habitats can be characterized by the highest level of population dynamics. B. jawlowskii plays the greatest role in the herpetobiont grouping in swamp and oak forest habitats (6.7% and 4.6% respectively). In other types of habitat this species composes 0.1–3.5% of the total abundance of this group. The highest abundance dynamic was reached by the Julidae cenopopulations which inhabit the swamp, oak forest and meadow habitats. B. jawlowskii occupies a relatively significant share in the herpetobiont communities of these habitats. Thus, the indicators of absolute number of this species and its relative participation in the herpertobiont grouping indicate the preference of this species for marsh, oak forest and meadow habitats. These habitats can be characterized by an excessive or moderate level of edaphotopic humidification. The ecosystem of the steppe zone of Ukraine is subject to significant human impact. In nature reserves, this effect is minimized, which permits research to be conducted on regimes of natural population dynamics. We established that B. jawlowskii inhabits all habitats investigated within the arena zone of the Dnepr river. This indicates that this is an environmentally flexible Julidae species. The population dynamics of B. jawlowskii can be characterized by three distinct periods: spring-summer, summer and autumn. Each of these periods is characterized by a distinct population dynamic, but throughout the study period the metapopulation of B. jawlowskii remained stable. This is due to the redistribution of representatives of the biotopical metapopulation over a wide range. The conservation regime within the "Dnieper-Orilsky" Nature Reserve had a positively effect on the state of the B. jawlowskii population. Within the reserve on the territory of the arena of the Dnepr river B. jawlowskii uses a wide variety of types of habitats.
Synopsis of the genus Anopteris (Pteridophyta, Pteridaceae)
Authors: Vaganov A. V. , Shmakov A. I. , Friesen N. 
Published: November 2016

In the article a synopsis of the genus Anopteris (Prantl) Diels is given. The synopsis of Anopteris includes three species. For each species, the Latin name, basionym, nomenclatural citation, synonyms, information on locus classicus, type, habitat, and distribution are given. An original key for identification of the species of Anopteris is also prepared. The localities of occurrence of each species are precisely given.
Influence of water infusion of medicinal plants on larvae of Strongyloides papillosus (Nematoda, Strongyloididae)
Authors: Boyko A. A. , Brygadyrenko V. V. 
Published: November 2016

One of the most common nematodes of ruminants is Strongyloides papillosus (Wedl, 1856). Disease caused by these parasites brings economic losses to livestock operations. Therefore it is necessary to control their numbers. The eggs and three larval stages of S. papillosus live in the environment, while the fourth, fifth and mature individuals live in host organisms. Control of these parasites is necessary at all stages of development, including the free-living stage. An experiment on changes in the number strongiloids under the influence of environmental factors was carried out using aqueous extracts of medicinal plants. In the laboratory experiment we researched the effect on the survival of invasive and noninvasive types of S. papillosus larvae of 24 hours exposure at different doses to Artemisia absinthium Linnaeus, 1753, Artemisia annua Linnaeus, 1753, Echinacea purpurea (Linnaeus, 1753) Moench, 1794, Matricaria chamomilla Linnaeus, 1753, Tanacetum vulgare Linnaeus, 1753, Salvia sclarea Linnaeus, 1753, Levisticum officinale W.D.J. Koch, 1824, Petroselinum crispum (Miller, 1768) Nyman ex A.W. Hill, 1925. The death of 50% of S. papillosus invasive larvae was registered at 464 ± 192 mg/l concentration of aqueous extract of S. sclarea inflorescences. The greatest effect up-on the non-invasive larvae was caused by aqueous extracts of inflorescences of S. sclarea, M. chamomilla and seeds of P. crispum: at concentrations of 327 ± 186, 384 ± 155 and 935 ± 218 mg/l, respectively, 50% of non-invasive larvae died. According to the results of the research, we suggest further study of the nematocidal activity of combinations, contained in the aboveground parts, of clary sage (S. sclarea), camomile (M. chamomilla) and seeds of parsley (P. crispum), and also experimental usage of these species in the fodder compound for cattle, sheep, goats and pigs on experimental farms.
Variation in morphological characters of the dice snake Natrix tessellata (Reptilia, Colubridae) in the Central and Southern Dnieper River Area
Authors: Yermolenko S. V. , Hahut A. M. , Gasso V. Y. 
Published: November 2016

The dice snake Natrix tessellata (Laurenti, 1768) is one of the common snake species in the Southern Palaearctic and North Africa. In the Central and Southern Dnieper River basin the dice snake is one of the most numerous and characteristic species. Relative isolation and the varying influence of environmental factors are known to be a motive force behind epigenetic divergence between populations. In populations of N. tessellata this divergence can be manifest in the form of morphometric and phenetic peculiarities. Research into these parameters of dice snake populations in terms of anthropogenic influence on habitats along a latitude gradient is essential. In the Central and Southern parts of the Dnieper River basin such studies are of great importance for the monitoring of populations and for further indicative purposes. The aim of our study is to characterize the morphometric features of N. tessellata in this area and to determine the variability of these parameters in the snake populations under varying intensities of anthropogenic influence. The snakes were collected in 2013–2016 from the territory adjacent to the Prydniprovska Thermal Power Plant, from the Maiorova gully (Maiorka village, Dnipropetrovsk region) and the National Nature Park “Velykyi Lug” (Zaporizhia region). The following morphometric parameters were recorded: snout-vent length (SVL), tail length (TL), the number of ventral scales (V), and the number of subcaudal scales (SBC), the number of scale rows at mid-body (MBS), the number of supralabial scales (SLS), the number of temporal scales (TS), and the number of postocular scales (POS). All the tested parameters generally correspond to the limits known for this species from the literature. Nevertheless , some geographic peculiarities in variability of the tail length and number of subcaudal scales were identified. According to data from the literature, the southern populations of the dice snake are distinguished by an increase in the number of subcaudal scales . The studied dice snake populations were characterized by some sexual dimorphism in the snout-vent length, tail length and the number of subcaudal scales. A higher percentage of bilateral asymmetry in snakes from the population close to the Prydniprovska TPP was found. Such changes are usually considered to be an indicator of a certain destabilization of the embryogenesis.
Clinicopathologic features of ameloblastoma in southern Brazil: a 55-year single-center analysis
Authors: De Carvalho Ana Luisa Homem , Martins Manoela Domingues , Da Rosa Francinne Miranda , Saudades Annemarie , Filho Manoel Sant´Ana 
Published: November 2016

Objective: Ameloblastoma is a benign odontogenic tumor (OT) originating from the proliferation of odontogenic epithelium without involvement of the mesenchymal tissue. The aims of the present study were to perform a retrospective analysis of ameloblastoma, compare the findings with those described in previous studies and outline the epidemiological profile of this type of tumor in southern Brazilian population.Methods: Cases of ameloblastoma were retrieved between 1954 and 2009 from a single center and analyzed for age, gender, ethnic, primary site and histological type. Cases were classified according to the 2005 WHO classification of OTs.Results: Descriptive statistical analysis of the data was performed, with the determination of the frequency of the categorical variables. Eighty-nine cases of ameloblastoma were found: 78 cases of solid tumor (88.6%), two cases of desmoplastic tumor (2.2%) and nine cases of the unicystic tumor (10.2%). There was a predominance of the female gender (58.4%) and Caucasian ethnicity (62.9%). Most ameloblastomas were located in the mandible (78.6%) and 40.2% occurred prior to the third decade of life.Conclusion: The present findings are in agreement with those reported in previous studies carried out in other states of Brazil and support that notion that there may be a geographical influence on the profile of patients affected by ameloblastoma.