bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft genome sequences of three Xanthomonas translucens pathovar reference strains (pv. arrhenatheri, pv. poae and pv. phlei) with different specificities for forage grasses
Authors: Hersemann Lena , Wibberg Daniel , Widmer Franco , Vorhölter Frank-Jörg , Kölliker Roland 
Published: August 2016

AbstractAs causal agents of bacterial wilt in pastures and meadows, bacteria of the species Xanthomonas translucens are a serious issue in forage grass production. So far, only little is known about host-pathogen interactions at the molecular level and the lack of comprehensive genome data impeded targeted breeding strategies towards resistant forage grass cultivars. Here we announce the draft genome sequences of three grass-pathogenic Xanthomonas translucens pathotype strains, i.e. pv. arrhenatheri LMG 727, pv. poae LMG 728 and pv. phlei LMG 730 isolated from Arrhenatherum elatius (L.) P. Beauv. ex J. Presl & C. Presl (Switzerland), Poa trivialis L. (Switzerland) and Phleum pratense L. (Norway), respectively. The genomes of all three strains revealed a non-canonical type III secretion system and a set of 22 type III effectors as common virulence-related traits. Distinct inter-pathovar differences were observed for the lipopolysaccharide biosynthesis gene cluster and the presence of nonribosomal peptide synthetases.
The complete genome sequence of the rumen methanogen Methanobrevibacter millerae SM9
Authors: Kelly William J. , Pacheco Diana M. , Li Dong , Attwood Graeme T. , Altermann Eric , Leahy Sinead C. 
Published: August 2016

AbstractMethanobrevibacter millerae SM9 was isolated from the rumen of a sheep maintained on a fresh forage diet, and its genome has been sequenced to provide information on the phylogenetic diversity of rumen methanogens with a view to developing technologies for methane mitigation. It is the first rumen isolate from the Methanobrevibacter gottschalkii clade to have its genome sequence completed. The 2.54 Mb SM9 chromosome has an average G + C content of 31.8 %, encodes 2269 protein-coding genes, and harbors a single prophage. The overall gene content is comparable to that of Methanobrevibacter ruminantium M1 and the type strain of M. millerae (ZA-10T) suggesting that the basic metabolism of these two hydrogenotrophic rumen methanogen species is similar. However, M. millerae has a larger complement of genes involved in methanogenesis including genes for methyl coenzyme M reductase II (mrtAGDB) which are not found in M1. Unusual features of the M. millerae genomes include the presence of a tannase gene which shows high sequence similarity with the tannase from Lactobacillus plantarum, and large non-ribosomal peptide synthase genes. The M. millerae sequences indicate that methane mitigation strategies based on the M. ruminantium M1 genome sequence are also likely to be applicable to members of the M. gottschalkii clade.
LysoPCs induce Hck- and PKCδ-mediated activation of PKCγ causing p47phox phosphorylation and membrane translocation in neutrophils
Authors: Kelher Marguerite R , McLaughlin Nathan J D , Banerjee Anirban , Elzi David J , Gamboni Fabia , Khan Samina Y , Meng Xianzhong , Mitra Sanchayita , Silliman Christopher C 
Published: August 2016

Abstract
Lysophosphatidylcholines (lysoPCs) are effective polymorphonuclear neutrophil (PMN) priming agents implicated in transfusion-related acute lung injury (TRALI). LysoPCs cause ligation of the G2A receptor, cytosolic Ca2+ flux, and activation of Hck. We hypothesize that lysoPCs induce Hck-dependent activation of protein kinase C (PKC), resulting in phosphorylation and membrane translocation of 47 kDa phagocyte oxidase protein (p47phox). PMNs, human or murine, were primed with lysoPCs and were smeared onto slides and examined by digital microscopy or separated into subcellular fractions or whole-cell lysates. Proteins were immunoprecipitated or separated by polyacrylamide gel electrophoresis and immunoblotted for proteins of interest. Wild-type (WT) and PKCγ knockout (KO) mice were used in a 2-event model of TRALI. LysoPCs induced Hck coprecipitation with PKCδ and PKCγ and the PKCδ:PKCγ complex also had a fluorescence resonance energy transfer (FRET)+ interaction with lipid rafts and Wiskott-Aldrich syndrome protein family verprolin-homologous protein 2 (WAVE2). PKCγ then coprecipitated with p47phox. Immunoblotting, immunoprecipitation (IP), specific inhibitors, intracellular depletion of PKC isoforms, and PMNs from PKCγ KO mice demonstrated that Hck elicited activation/Tyr phosphorylation (Tyr311 and Tyr525) of PKCδ, which became Thr phosphorylated (Thr507). Activated PKCδ then caused activation of PKCγ, both by Tyr phosphorylation (Τyr514) and Ser phosphorylation, which induced phosphorylation and membrane translocation of p47phox. In PKCγ KO PMNs, lysoPCs induced Hck translocation but did not evidence a FRET+ interaction between PKCδ and PKCγ nor prime PMNs. In WT mice, lysoPCs served as the second event in a 2-event in vivo model of TRALI but did not induce TRALI in PKCγ KO mice. We conclude that lysoPCs prime PMNs through Hck-dependent activation of PKCδ, which stimulates PKCγ, resulting in translocation of phosphorylated p47phox.
Improving Oral Oncolytic Patient Self-Management
Authors: McNamara Elaine , Redoutey Lindsey , Mackler Emily , Severson Jane A. , Petersen Laura , Mahmood Tallat 
Published: August 2016

Purpose:Managing patients who are taking oral oncolytics is challenging because of the changing paradigm from frequent supervision during intravenous therapy to periodic observation with oral administration of drugs. We joined the Michigan Oncology Quality Consortium (MOQC) Oral Oncolytics Collaborative in 2013 to identify opportunities for improvement in this area.Methods:We completed MOQC’s baseline self-assessment and performed an audit of medical records for 25 patients prescribed an oral oncolytic from May 2011 to July 2013. We implemented the following MOQC resources: a tracking system for patients taking oral oncolytics, patient education with drug-specific self-care guidelines, use of a modified Edmonton Symptom Assessment Scale, and a medication adherence questionnaire to be used on scheduled follow-up calls and return visits. We modified our workflow to include a standard teaching session and consistent follow-up phone calls. We conducted a retrospective postimplementation medical records audit from August 2013 to September 2014.Results:Baseline self-assessment revealed lack of start date documentation and lack of consistent follow-up. A baseline medical records audit showed that 48% of patients discontinued their medication without consulting their physician, and start date documentation was available for only 52% of patients. After participating in the quality initiative, 100% of patients sampled had a documented start date, and no patients discontinued their drug on their own. Seventeen percent had a dose reduction as a result of toxicity, as directed by the physician.Conclusion:The introduction of new office procedures to easily identify all patients receiving oral therapy and improvement in patients’ ability to manage symptoms at home with the use of self-care guidelines contributed to an improvement in managing patients who are taking oral oncolytics.
Association of Practice-Level Hospital Use With End-of-Life Outcomes, Readmission, and Weekend Hospitalization Among Medicare Beneficiaries With Cancer
Authors: Clough Jeffrey D. , Strawbridge Larisa M. , LeBlanc Thomas W. , Hammill Bradley G. , Kamal Arif H. 
Published: August 2016

Purpose: To determine the relationships between hospital use of treating oncology practices and patient outcomes. Patients and Methods: Retrospective analysis of 397,646 Medicare beneficiaries who received anticancer therapy in 2012. Each beneficiary was associated with a practice; practices were ranked on the basis of risk-adjusted hospital use, that is, inpatient intensity. Outcomes included 30-day readmission, weekend admissions, intensive care unit stays in the last month of life, and hospice stay of ≥ 7 days. Outcomes were measured for each quartile of practice-level inpatient intensity. We fit multivariable logistic regression models to calculate adjusted odds ratios (ORs) for each outcome for each quartile of inpatient intensity. Results: Total 30-day readmissions were 22.8% and 31.9% (OR, 1.45; 95% CI, 1.39 to 1.50) for patients in practices with the lowest versus highest quartiles of inpatient intensity, respectively; unplanned readmissions were 19.8% and 27.1% (OR, 1.36; 95% CI, 1.31 to 1.41), respectively. The proportion of admissions that occurred on weekends was similar across quartiles. Patients of practices in the highest quartiles of inpatient intensity had higher rates of death in an ICU stay in the last month of life (25.5% versus 18.0%; OR, 1.33; 95% CI, 1.19 to 1.49) and a lower rate of hospice stay of at least 7 days (50.9% to 42.5%; OR, 0.79; 95% CI, 0.74 to 0.86). Conclusion: Medical oncology practices that seek to reduce hospitalizations should consider focusing initially on processes related to end-of-life care and care transitions.
Risk Factors for Unintended Dural Puncture in Obstetric Patients: A Retrospective Cohort Study
Authors: Orbach-Zinger Sharon , Ashwal Eran , Hazan Lilach , Bracco Danielle , Ioscovich Alex , Hiersch Liran , Khinchuck Andrey , Aviram Amir , Eidelman Leonid A. 
Published: August 2016

BACKGROUND:
Unintended dural puncture (UDP) is one of the main risks of epidural analgesia, with a reported incidence of approximately 1.5% among the obstetric population. UDP is associated with maternal adverse outcomes, with the most frequent adverse outcome being postdural puncture headache (PDPH). Our retrospective cohort study objective was to identify demographic and obstetric risk factors that increase the risk of unintentional dural puncture as well as describing the obstetric outcome once a dural puncture has occurred.

METHODS:
We retrospectively reviewed all cases of UDPs during attempted vaginal delivery between the years 2004 and 2013 in a single Israeli hospital. Each UDP case was matched with the 2 parturients who received epidural analgesia before and 2 parturients after performed by the same anesthesiologist (control group). Demographic, anesthetic, and obstetric variables were compared between the UDP and control groups.

RESULTS:
Out of 46,668 epidural procedures, 177 cases of UDPs were documented (0.4%). One hundred seven women (60.5%) developed PDPH, and 38 (35.5%) required an epidural blood patch. In multivariate logistic regression, the degree of cervical dilation in centimeters at the time of epidural insertion was associated with an increased rate of UDP (P < .001). Multiparity was associated with PDPH after UDP (P = .004). Women with UDP had longer length of hospital stay than those without UDP (P < .001).

CONCLUSIONS:
UDP, an uncommon complication, is associated with obstetric factors. Nevertheless, it does not seem to be associated with adverse obstetric outcomes except for prolonged duration of hospital stay.
The Impact of Residual Neuromuscular Blockade, Oversedation, and Hypothermia on Adverse Respiratory Events in a Postanesthetic Care Unit: A Prospective Study of Prevalence, Predictors, and Outcomes
Authors: Stewart Paul A. , Liang Sophie S. , Li Qiushuang Susan , Huang Min Li , Bilgin Ayse B. , Kim Dukyeon , Phillips Stephanie 
Published: August 2016

BACKGROUND:
Residual neuromuscular blockade (RNMB) has been linked to adverse respiratory events (AREs) in the postanesthetic care unit (PACU). However, these events are often not attributed to RNMB by anesthesiologists because they may also be precipitated by other factors including obstructive sleep apnea, opioids, or hypnotic agents. Many anesthesiologists believe RNMB occurs infrequently and is rarely associated with adverse outcomes. This study evaluated the prevalence and predictors of RNMB and AREs.

METHODS:
This prospective cohort study included 599 adult patients undergoing general anesthesia who received neuromuscular blocking agents. Baseline demographic, surgical, and anesthetic variables were collected. RNMB was defined as a train-of-four ratio below 0.90 measured by electromyography on admission to the PACU. AREs were defined based on the modified Murphy’s criteria.

RESULTS:
RNMB was present in 186 patients (31% [95% confidence interval (CI), 27%–35%]) on admission to the PACU. One or more AREs were experienced by 97 patients (16% [95% CI 13–19]). AREs were more frequent in patients with RNMB (21% vs 14%, P = .033). RNMB was significantly associated with age (adjusted relative risk [RR], 1.17 [95% CI, 1.06–1.29] per 10-year increase), type of operation (adjusted RR, 0.59 [95% CI, 0.34–0.99] for laparoscopic surgery compared with open abdominal surgery), and duration of operation (adjusted RR, 0.59 [95% CI, 0.39–0.86] for ≥90 minutes compared with <90 minutes). Using multivariate logistic regression, AREs were found to be independently associated with decreased level of consciousness (adjusted RR, 4.76 [95% CI, 1.49–6.76] for unrousable/unconscious compared with alert/awake) and lower core temperature (adjusted RR, 1.43 [95% CI, 1.04–1.92] per 1°C decrease). Although univariate analysis found a significant association between AREs and RNMB, the significance became borderline after adjusting for other covariates (adjusted RR, 1.46 [95% CI, 0.99–2.08]).

CONCLUSIONS:
The prevalence of RNMB in the PACU was >30%. Older age, open abdominal surgery, and duration of operation <90 minutes were associated with increased risk of RNMB in our patients. Our RR estimate for AREs was highest for depressed level of consciousness. When AREs occur in the PACU, potentially preventable causes including RNMB, hypothermia, and reduced level of consciousness should be readily identified and treated appropriately. Delaying extubation until the patient is awake and responsive may reduce AREs.
The Promise (and Pitfalls) of Public Health Policy Surveillance
Authors: Hodge James G. 
Published: August 2016

Abstract
Though public health policy surveillance is an integral tool in correlating the law to scientifically based public health law studies, drawing accurate legal conclusions from collected data can be challenging. Data may be of poor quality, inaccessible to law and policy makers, or inapplicable to other jurisdictions over time and place. As Burris et al. (2016) advocate, modern, sophisticated, and interactive data collection systems would render more precise legal analysis tied to public health improvements. Although policy surveillance is promising, public health officials, health care providers, attorneys, and researchers must be skilled and prepared to successfully navigate and resolve potential pitfalls for its benefits to be fully realized. Among the significant challenges related to policy surveillance are: (1) timing; (2) agenda setting; (3) predictable misuse; and (4) politics inherent in a federalist public health legal infrastructure. As public health data infrastructure is developed, better legal approaches must be simultaneously crafted to achieve optimal public health outcomes.
Public Health Law and Institutional Vaccine Skepticism
Authors: Parasidis Efthimios 
Published: August 2016

AbstractVaccine-hesitant parents are often portrayed as misinformed dilettantes clinging to unscientific Internet chatter and a debunked study that linked the MMR vaccine and autism. While this depiction may be an accurate portrayal of a small (but vocal) subset, scholars have unearthed a more complex picture that casts vaccine hesitancy in the context of broader notions of lack of trust in government and industry. At the same time, commentators have highlighted limitations of the vaccine injury compensation program and US Supreme Court Justices Sonia Sotomayor and Ruth Bader Ginsburg have argued that preemption laws that provide vaccine manufacturers with broad legal immunities create “a regulatory vacuum in which no one ensures that vaccine manufacturers adequately take account of scientific and technological advancements when designing or distributing their products.” In short, the discussions surrounding vaccine hesitancy that dominate public discourse detract from serious debate as to whether amendments to vaccine-related laws can address the limitations of the existing framework governing immunizations. This commentary examines these issues through a public health law lens.
Medicaid Expansion in a Litmus State: The Missouri Struggle
Authors: Brasfield James 
Published: August 2016

Abstract
For a century Missouri was a bellwether state in presidential elections, always picking the winner. Since 2008 it has been experiencing a partisan divide along urban/rural lines with President Obama losing the state twice. The battle over Medicaid expansion found a Democratic governor unable to convince a Republican legislative majority to support ACA-based expansion. The more highly partisan legislative environment has rendered traditional bargaining and negotiations impossible on the controversial question of Medicaid expansion.
Despite supportive advocacy by hospitals and the business community, the Republican legislative leaders have opposed any movement on Medicaid expansion over the past four years. There will be a new occupant in the governor's mansion in 2017, which may create a fork in the road. Democrats are unlikely to regain a legislative majority, and one path is continued Republican refusal to consider expansion. The other path features the new governor responding to the national 2016 election outcome, and creating the prospects for a deal, perhaps around a waiver plan.
Phylogenetic relationships at the base of Ericaceae: Implications for vegetative and mycorrhizal evolution
Authors: Freudenstein John V. , Broe Michael B. , Feldenkris Emily R. 
Published: August 2016

AbstractThe relationships among pyroloids, monotropoids, arbutoids and the remainder of Ericaceae continue to be unresolved even though significant progress has been made in understanding the phylogenetic structure of the family. This means that, in addition to uncertainty in classification, evolutionary patterns in vegetative structure and mycorrhizal diversity in the family have also been uncertain. This study analyzes nuclear, plastid and mitochondrial sequence data in order to provide a basis for classification and character analysis. Parsimony and maximum likelihood analyses of partitioned and combined data are largely in agreement; organellar data differ from nuclear data with respect to the position of pyroloids. Based on nuclear, plastid and combined data analyses, monotropoids are sister to arbutoids, and these are together sister to pyroloids, and thence to a core Ericaceae and finally to Enkianthus. This suggests that a reduction in woodiness and increased mycotrophy has occurred in parallel in pyroloids and monotropoids and that the arbutoid and ericoid mycorrhizal types were each derived from the arbuscular type in Ericaceae.
Platform Integration and Demand Spillovers in Complementary Markets: Evidence from Facebook’s Integration of Instagram
Authors: Li Zhuoxin , Agarwal Ashish 
Published: August 2016

Social media platform owners often choose to provide tighter integration with their own complementary applications (i.e., first-party applications) as compared to that with other complementary third-party applications. We study the impact of such integration on consumer demand for first-party applications and competing third-party applications by exploring Facebook’s integration of Instagram, an application in its photo-sharing application ecosystem. We find that consumers obtain additional value from Instagram after its integration with Facebook, leading to a large increase in the use of Instagram for Facebook photo sharing. Further, we find that the growth of Instagram’s user base has a positive spillover effect on big third-party applications and a negative spillover effect on small third-party applications in Facebook’s photo-sharing ecosystem. As a result, while small third-party applications face reduced demand after integration, big third-party applications experience a small increase in demand. Thus, the overall demand for the entire photo-sharing application ecosystem actually increases, which suggests that Facebook’s integration strategy benefits the complementary market overall. Our results highlight the role of platform integration for first-party applications and the application ecosystem overall, and they have implications for strategic management of first-party applications in the presence of third-party applications.This paper was accepted by Anandhi Bharadwaj, information systems.
Słowotwórstwo nazwisk mieszkańców wsi we wrzesińskich księgach chrztu z XVIII wieku
Authors: Sieradzki Andrzej 
Published: August 2016

The article studies name derivatives referring to rural population recorded in the eigh­teenth-century baptismal books of Września. The core of the study are 164 surnames formed by the addition of the suffixes -ski, -ewicz, -owicz as well as the basic -k-. The studies completed demonstrate that in the process of creation of names, over the entire 18th century, the most dominating were the word formation models with suffixes including the basic -k-. Within this category the suffix -ak was the most active element. Of marginal importance (only in the creation of formal derivatives) was the suffix -ski and the derived suffixes. Only 20 formations were created with their use during the whole century. On the basis of the analysis of the documents, it can be assumed that in the rural area near Września there were no word-formative models with the formatives -ewicz and -owicz. The attested surnames (7), recorded between 1753 and 1760, must have been brought from other areas.
Polski dyskurs patriotyczny w prasie i w pismach okolicznościowych z 2. połowy XVIII wieku
Authors: Siuciak Mirosława 
Published: August 2016

The article examines the process of shaping of the Polish patriotic discourse, which was determined by such events of the 18th century as the Bar Confederation, the Great Sejm proceedings, and the Kościuszko Uprising. In a situation in which Poland was threatened with the invasion of surrounding world powers and in the face of impending armed struggle for sovereignty, the then most important values uniting the Polish nation were formulated. The first entity to define them were the Bar confederates, who fought against the Russian army in defence of their fatherland, religion, freedom, and independence. In the 1790s the patriotic discourse shifted to the press covering the proceedings of the Great Sejm, and later to newspaper coverage of the Kościuszko Uprising. During these events, the freedom demands were altered because the conservative Sarmatian republicanism had been replaced with ‚new’ republicanism – characterised by social radicalism and supporting the separation of church and state. Despite some semantic shifts, for more than 200 years the most significant values constituting the Polish patriotic discourse have been: fatherland, freedom, and independence.
Słownik języka Jana Chryzostoma Paska odczytany feministycznie
Authors: Sobolewska Katarzyna 
Published: August 2016

The feminist interpretation of The Dictionary of the language of Jan Chryzostom Pasek consisted in searching for information on the status and position of women during the baroque period, and in tracing these lexicographic tools, which revealed the hierarchy of gender related values employed by its editors. The analysis focused on lexicographic articles describing persons of female gender in juxtaposition with their male counterparts. The Dictionary reflects all of the central features of patriarchal culture of Polish Sarmatia and the entailed asymmetry of gender roles. However, as the gender category has not been sufficiently accounted for in the lexicographic treatment of personal nouns, The Dictionary does not appreciate some of the cultural content.