bluuebunny/crossref_metadata_2025_split huggingface dataset:
TropD-Detector: A CRISPR/LbCas12a-Based system for rapid and sensitive screening ofTrypanosoma cruziin Chagas vectors and reservoirs
Authors: Ortiz-Rodríguez Luis A. , Cabanzo Rafael , Jaimes-Dueñez Jeiczon , Mendez-Sanchez Stelia C. , Duque Jonny E 
Published: October 2024

AbstractChagas disease, also known as American Trypanosomiasis, is a zoonosis with global distribution caused by the parasiteTrypanosoma cruzi, primarily transmitted through the feces of infected triatomines. The emergence of new cases in non-endemic areas highlights the importance of early pathogen detection in vectors and reservoirs to generate effective control strategies and establish preventive policies. The objective of this study was to design and validate a detection system ofT. cruzibased on specific DNA cleavage and collateral activation using the CRISPR/LbCas12a system, targeting the genes Cytochrome B (Cytb), 18S ribosomal subunit (SR18s), and histone (H2A). This system was validated for their uses in both vectors and reservoirs of the parasite. The first step was to conduct a bioinformatic analysis of the target genes and design RNA guides for each cleavage site and primers to amplify the target region through PCR. Subsequently, we sequenced the amplified DNA target and validated the detection system usingT. cruziDNA extracted from naturally infectedRhodnius pallescensin the metropolitan area of Bucaramanga, Colombia. After standardizing the method, we tested the CRISPR/Cas system with Silvio X10/4 laboratory strain ofT. cruziand scaled up to blood samples of naturally infectedDidelphis marsupialis. As a result, we observed the DNA cleavage employing the CRISPR/Cas system withCytbguide with a sensitivity of up to 0.0475 ng/uL of provided DNA. Sequencing of theCytbgene showed no mutations in the cleavage site. However, point mutations and indels were found inSR18SandH2A, avoiding the formation of the CRISPR/LbCas12 complex. Furthermore, we introduce the design of a fluorescent detection prototype with CRISPR/LbCas12a called “Tropical Diseases Detector” (TropD-Detector). This device operates with an excitation wavelength of 480 nm emitted by an LED and a high-pass light filter with a cutoff wavelength of 500 nm. We detected positive samples using any photographic camera system. The TropD-Detector provides a visual, viable, and sensitive method for detectingT. cruziin both vectors and reservoirs from endemic areas.
TaRTLEt: Transcriptionally-active Riboswitch Tracer Leveraging Edge deTection
Authors: Kshatriya Sachit , Bagby Sarah C. 
Published: October 2024

ABSTRACTStructured RNAs have emerged as a major component of cellular regulatory systems, but their mechanism of action is often poorly understood. Riboswitches are structured RNAs that allosterically regulate gene expression through any of several different mechanisms.In vitroapproaches to characterizing mechanism are costly, low-throughput, and must be repeated for each individual riboswitch locus of interest. Bioinformatic methods promise higher throughput; despite robust computational identification of riboswitches, however, computational classification of riboswitch mechanism has so far been both model-bound, relying on identification of sequence motifs known to be required for specific models of riboswitch activity, and empirically untested, with predictions far outpacing biological validation. Here, we introduceTaRTLEt(Transcriptionally-active Riboswitch Tracer Leveraging Edge deTection), a new high-throughput tool that recoversin vivopatterns of riboswitch-mediated transcription termination from paired-end RNA-seq data using edge detection methods.TaRTLEtsuccessfully extracts transcription termination signals despite numerous sources of biological and technical noise. We tested the effectiveness ofTaRTLEton riboswitches identified from a wide range of sequenced bacterial taxa by utilizing publicly available paired-end RNA-seq readsets, finding broad agreement with previously publishedin vitrocharacterization results. In addition, we useTaRTLEtto infer thein vivoregulatory mechanism of uncharacterized riboswitch loci from existing public data.TaRTLEtis available on GitHub and can be applied to paired-end RNA-seq datasets from isolates or complex communities.
Advantage of the F(ab)’2 fragment over IgG for RIT and PRIT
Authors: Tabaja Zainab , Diab Maya , Nguyen Frédérique , Maurel Catherine , Pecorari Frédéric , Lambot Sylvia , Davodeau François 
Published: October 2024

AbstractRadioimmunotherapy (RIT) has proved clinically effective in the treatment of relapsed or consolidating NHL after chemotherapy. However, it is still at the clinical trial stage for the treatment of solid tumors. The efficacy of the treatment on this type of tumor is limited by hematological toxicity, which restricts the activities injected. Pre-targeted radioimmunotherapy (PRIT) enables radiolabeling to be carried out in vivo at a time when the quantities of antigen-targeted vector reach a maximum level in the tumor, but have largely decayed in the bloodstream, thus limiting hematological toxicity. This therapeutic approach would make it possible to use higher radiopharmaceutical activities than those used in RIT, thereby increasing the doses deposited in the tumor and the therapeutic response.Despite encouraging preclinical trials, this approach has failed to live up to expectations in terms of clinical applications. In the context of PRIT using the Inverse Electron Demand Diels Alder (IEDDA) reaction as a method for radiolabeling tumor-targeted vectors, a major research effort has been made to improve the quality of in vivo radiolabeling using tetrazine (Tz) and transcycloctene (TCO) variants whose stability and reactivity have been optimized. Most trials in this field have been carried out using antibodies which, thanks to their long biological half-life, enable prolonged irradiation of tumors. Low-molecular-weight vectors such as affinity proteins or antigen-specific antibody fragments (VHH,scFv, F(ab)’) have the advantage of being rapidly eliminated from circulation by renal filtration, but on the other hand, the quantities and residence times of these vectors in the tumor are reduced compared with IgG.F(ab)’2 antibody fragments have properties intermediate between those of IgG and low-molecular-weight vectors that could be suitable for PRIT approaches, but they have not yet been evaluated in PRIT using IEDDA. In preparation for a preclinical trial of radioimmunotherapy of triple-negative breast cancer in spontaneously affected dogs, we compared the biodistribution of IgG and its F(ab)’2 fragment targeting canine CD138 in a xenogeneic model of canine breast cancer and in a mouse model of colon cancer expressing human ACE antigen. This analysis shows that the use of F(ab)’2 fragments improves the therapeutic index compared with IgG. F(ab)’2 fragments seem particularly well suited to tumor targeting in PRIT using short-lived isotopes such as 211At.
The influence of the pre-membrane and envelope proteins on structure, pathogenicity and tropism of tick-borne encephalitis virus
Authors: Rosendal Ebba , Bisikalo Kyrylo , Willekens Stefanie M.A. , Lindgren Marie , Holoubek Jiří , Svoboda Pavel , Lappalainen Amanda , Könighofer Ebba , Mirgorodskaya Ekaterina , Nordén Rickard , Morini Federico , Rosenbaum William , Růžek Daniel , Ahlgren Ulf , Anastasina Maria , Merits Andres , Butcher Sarah J , Nilsson Emma , Överby Anna K 
Published: October 2024

AbstractTick-borne encephalitis virus (TBEV) is a neurotropic flavivirus that causes thousands of human infections annually. Viral tropism in the brain is determined by the presence of necessary receptors, entry factors and the ability of the virus to overcome host defenses. The viral structural proteins, pre-membrane (prM) and envelope (E), play an important role in receptor binding, membrane fusion, particle maturation, and antibody neutralization. To understand how these proteins influence virus distribution and tropism in the brain, we generated a chimeric virus harboring the prM and ectodomain of E from TBEV in the background of the low pathogenic Langat virus (LGTV). We solved the atomic structures of both the chimeric virus and LGTV to compare them to the known TBEV structure. We show that this chimeric virus remains low-pathogenic, while being structurally and antigenically similar to TBEV. Using 3D optical whole brain imaging combined with immunohistochemistry, we found that both LGTV and the chimeric virus primarily infect cerebral cortex, with no significant differences in their localization or tropism. In contrast, TBEV shows high infection of the cerebellum and strong preference towards Purkinje cells, indicating that the non-structural proteins are important for determining TBEV tropism in the brain. Together, this provides new insights into the roles of the structural and non-structural proteins of tick-borne flaviviruses.
Emergence and host range expansion of an epidemic lineage ofRalstonia solanacearum
Authors: Evseeva Daria , Pecrix Yann , Kucka Marek , Weiler Catrin , Franzl Carolin , Vlková-Žlebková Markéta , Colombi Elena , Chan Yingguang Frank , Poussier Stéphane , Wicker Emmanuel , McCann Honour 
Published: October 2024

AbstractMembers of theRalstonia solanacearumspecies complex are soil-borne pathogens of important crop plants. Strain-level host specialisation is considered typical, yet outbreak monitoring in Martinique revealed the emergence of a new lineage with dramatically expanded host range. We show the outbreak was caused by the parallel expansion of lineages from two differentRalstoniaspecies. One lineage of Asian origin retains a signature of host specialisation, while the emergent broad host range lineage originated in the Americas. The presence of similar integrative and conjugative elements in both lineages suggests they are exchanging genetic material. The emergent lineage acquired genes encoding novel catalytic and metabolic functions, but shows little variation among virulence genes considered host specificity determinants. The host range expansion event was not caused by the evolution of a virulence strategy targeting host immunity, but is more likely due to the acquisition of novel substrate utilisation pathways enhancing pathogen fitness in soil or plant tissues.
STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells
Authors: Blanch-Asensio Albert , Ploessl Deon S. , Wang Nathan B. , Mummery Christine L. , Galloway Kate E. , Davis Richard P. 
Published: October 2024

AbstractTargeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, allele-specific, single-copy integration of two DNA payloads with 100% efficiency within one week. Notably, STRAIGHT-IN Dual leverages the STRAIGHT-IN platform to allow near-scarless cargo integration, facilitating the recycling of components for subsequent cellular modifications. Using STRAIGHT-IN Dual, we investigated how promoter choice and gene syntax influences transgene silencing, and demonstrate the impact of these design features on forward programming of hiPSCs into neurons. Furthermore, we designed a grazoprevir-inducible synZiFTR system to complement the widely-used tetracycline-inducible system, providing independent, tunable, and temporally controlled expression of both transcription factors and functional reporters. The unprecedented efficiency and speed with which STRAIGHT-IN Dual generates homogenous genetically engineered hiPSC populations represents a major advancement for synthetic biology in stem cell applications and opens opportunities for precision cell engineering.
Shared value in female entrepreneurship. A publicness frame in the field of knowledge sharing
Authors: Arena Claudia , Catuogno Simona , Paoloni Paola , Pastore Patrizia 
Published: October 2024

Purpose
While most research on female entrepreneurship has been conducted using the traditional private-for-profit logic, with the growing rise of hybrid and public organizational forms, the value of female entrepreneurial initiatives tends to be shared with society at large. Overcoming the traditional distinction between private vs public sectors, this paper relies on the publicness theory and aims to appreciate the shared value of female entrepreneurship by reviewing evidence from the field of knowledge sharing.

Design/methodology/approach
The authors conduct a three-phase systematic literature review on female entrepreneurship and knowledge sharing based on a sample of 188 articles for the period 2010–2022 retrieved from the three most extensive databases for evaluating scientific research (i.e. WoS, Scopus and Google Scholar) using a combination of relevant keywords within business and management domain. After the performance analysis of selected publications, the authors conduct a content analysis aimed at identifying the most recurrent theoretical framing, the impact of female entrepreneurship on value creation and the use of knowledge sharing. Finally, the authors conduct a thematic analysis using the theoretical perspective of the publicness to frame how different knowledge-sharing practices have been used in female entrepreneurship to create and spread public value.

Findings
The findings reveal that female entrepreneurship research has generally adopted managerial organizational and sociological theoretical frameworks, reported positive implications on value creation and has seldom exploited knowledge-sharing practices, with particular reference to the settings of low level of publicness. In addition, based on the development of the conceptual model, the authors suggest that the practice of networking in organizational structures characterized by a high level of political and/or economic authority makes the pattern of public value-sharing with the entire ecosystem easier.

Originality/value
The authors adopt an original approach that frames female entrepreneurship research within the lens of publicness theory to highlight the knowledge-sharing practices that allow the value created by female entrepreneurial initiatives to become a shared value in organizations characterized by high levels of political and/or economic authority. The proposed framework contributes to the research on entrepreneurship and knowledge sharing by providing a structured reference point to carry forward research on gender entrepreneurship and knowledge-sharing practices through the theoretical lens of the publicness.
The dark side of brand community: the role of brand identification, community identification, brand passion and shopping motivation
Authors: Dini Azizi Putri , Japutra Arnold , Arango Luis , Kim Joohee 
Published: October 2024

Purpose
This paper aims to investigate whether consumers’ identification with a brand community makes them more likely to engage in compulsive buying behavior. Specifically, this research shows that consumers are more likely to experience obsessive passion for brands they identify with if they are also part of brand communities, which, in turn, makes them more likely to engage in compulsive buying behavior.

Design/methodology/approach
An online survey (n = 533) among members of the Adorable Representative M.C. for Youth (ARMY) Bangtan Boys (BTS) brand community was conducted. A partial least squares approach was employed to test the validity and reliability of the measurement model as well as to assess the structural equation model.

Findings
Results show that brand identification affects harmonious and obsessive passion and that both relationships are moderated by brand community identification. Harmonious passion, in turn, has a positive effect on impulsive buying, whereas obsessive passion has a positive effect on both impulsive and obsessive-compulsive buying. Furthermore, consumer hedonic motivations moderate the relationship between (i) harmonious passion and impulsive buying and (ii) obsessive passion and obsessive-compulsive buying.

Originality/value
To the best of the authors’ knowledge, this work is the first to examine the potential negative impacts brand community identification has on compulsive buying behavior. Besides contributing to research on the negative aspects of brand community identification, by employing a model that distinguishes between two types of passion and compulsive buying, the study provides clarification on relationships suggested by prior studies.
Is Bowman’s paradox an empirical artifact? Evidence from Asian emerging countries
Authors: Khan Asad , Rehman Zia ur , Badshah Imtiaz , Khan Muhammad Ibrahim 
Published: October 2024

PurposeThis study aims to reconcile and address Bowman’s paradox empirical criticism from the lens of financial theory, corporate strategy and their econometric adversaries based on three issues, i.e. risk conceptualization, measurement and econometric modeling in Asian emerging countries (AEC).Design/methodology/approachThe study is conducted on panel data sampling from 2,872 firms across four Asian Emerging Countries (AEC) and employs a two-stage least squares (2SLS) estimation technique. We proposed a theoretical framework based on triangulation that outlines four risk-return relationships based on proxies derived from capital market and firm-level data and used different econometric models to answer Bowman’s paradox ongoing criticism.FindingsThe empirical results negate the empirical artifact viewpoint in AEC. The risk-return relationship estimated on firm accounting-based ratios or its combination with market-based measures supports Bowman’s paradox and thus upholds the corporate strategy point of view. Whereas the risk-return relationship based on market-based ratios upholds the financial theory point of view. However, the results are mixed when risk is subdivided into systematic and business risk. Our results are robust across standard deviation and semi-standard deviation-based measures of risk, and there is no evidence of a non-linear relationship.Originality/valueA compelling debate exists that Bowman’s paradox is an empirical artifact. We provide an innovative approach that aims to reconcile and address the ongoing debate by employing diverse risk-return proxies and econometric models in Asian emerging countries. Methodological issues such as endogeneity, sample biases, temporal fluctuations, downside risk variations, multiple moments of a variable and model misspecification are also addressed. This triangulation enhances the robustness of our analysis, providing a comprehensive perspective on AEC and laying the groundwork for future researchers to explore Bowman’s paradox through alternative measures and models.
Legacies from decolonizing methodologies
Authors: Vaughn Kēhaulani 
Published: October 2024

PurposeThis study aims to reflect and honor Linda Smith and her seminal work Decolonizing Methodologies.Design/methodology/approachReflecting on Linda Smith’s Decolonizing Methodologies: Research and Indigenous Peoples, I discuss my own application of Smith’s major contributions to my research, teaching- and community engaged work. I begin with my positionality and detail my journey as a Native Hawaiian teacher and researcher who has been influenced by the major tenants of Decolonizing Methodologies. After 25 years, Smith continues to empower Indigenous scholars and reminds us of the importance of the process of research and the necessary actions to collaborate with Indigenous communities towards knowledge production.FindingsReflections of how the tenants of Decolonizing Methodologies have influenced my own research, teaching and community engaged work.Research limitations/implicationsThis is my personal reflection and so it is limited.Practical implicationsOther Indigenous scholars can relate to my reflections of Linda Smith and Decolonizing Methodologies.Social implicationsMany of us can attest to the power of Decolonizing Methodologies.Originality/valueMy reflections as a Native Hawaiian woman will add value to the special issue.
A review on surface morphology and tribological behavior of titanium alloys via SLM processing
Authors: Kuntoğlu Mustafa , Salur Emin , Gupta Munish Kumar , Waqar Saad , Szczotkarz Natalia , Vashishtha Govind , Korkmaz Mehmet Erdi , Krolczyk Grzegorz M. , Aslan Abdullah , Binali Rüstem 
Published: October 2024

Purpose
Additive manufacturing became the most popular method as it enables the production of light-weight and high-density parts in effective way. Selective laser melting (SLM) is preferred by means of producing a component with good surface quality and near-net shape even if it has complex form. Titanium alloys have been extensively used in engineering covering a variety of sectors such as aeronautical, chemical, automotive and defense industry with its unique material properties. Therefore, the purpose of this review is to study the tribological behavior and surface integrity that reflects the thermal and mechanical performances of the fabricated parts.

Design/methodology/approach
This paper is focused on the tribological and surface integrity aspects of SLM-produced titanium alloy components. It is aimed to outline the effect of SLM process parameters on tribology and surface integrity first. Then, thermal, thermal heat, thermomechanical and postprocessing surface treatments such as peening, surface modification and coatings are highlighted in the light of literature review.

Findings
This work studied the effects of particle characteristics (e.g. size, shape, distributions, flowability and morphology) on tribological performance according to an extensive literature survey.

Originality/value
This study addresses this blind spot in existing industrial-academic knowledge and goals to determine the impact of SLM process parameters, posttreatments (especially peening operations) and particle characteristics on the SLMed Ti-based alloys, which are increasingly used in biomedical applications as well as other many applications ranging from automobile, aero, aviation, maritime, etc. This review paper is created with the intention of providing deep investigation on the important material characteristics of titanium alloy-based components, which can be useful for the several engineering sectors.