bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and verification of reference intervals for blood cell analysis in extremely high altitude
Authors: Yuan Zhimin , Zhuang Jian 
Published: October 2024

ObjectiveThis study aimed to establish the reference intervals for complete blood count (CBC) in healthy adults at very high altitudes.MethodsThis study recruited 4,863 healthy adults (3,598 males and 1,265 females) who received routine health check-ups at Ali District People’s Hospital from January 2019 to December 2021 through the LIS system. CBC was detected by the XT-4000i automatic cell analyzer and statistically analyzed by SPSS 23.0 software (between-groups comparison, diagnostic concordance comparison). In addition, 20 health check samples were collected in 2022 to validate the established reference intervals.ResultsThe CBC count showed a non-normal distribution in each item separately. The white blood cell (WBC), neutrophil (NEUT), lymphocyte (LYMPH), monocyte (MONO), eosinophil (EO), basophil (BASO), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean hemoglobin content (MCH), and mean erythrocyte hemoglobin concentration (MCHC) levels of healthy adults were significantly different from the national clinical reference range (p &lt; 0.05). There were significant differences between males and females in RBC, HGB, and HCT levels (p &lt; 0.05). The new reference intervals were less consistent with the expert consensus CBC reference intervals (p &lt; 0.05). Compared with the other reference intervals, the diagnostic value of the reference screening interval established was significantly higher in this study (p &lt; 0.05). The reference range established is verified by 20 independent samples from 2022, and the results are acceptable.ConclusionThis study preliminarily established reference intervals for complete blood counts of healthy adults at high altitudes in the Ali region of Tibet to provide a reference point for understanding routine blood markers in permanent residents of high-altitude environments and illustrate the need for regular establishment of laboratory reference intervals on a regular basis.
Genome-wide identification of the NAC family in Hemerocallis citrina and functional analysis of HcNAC35 in response to abiotic stress in watermelon
Authors: Cao Lihong , Wang Jinyao , Ren Sijia , Jia Yumei , Liu Yue , Yang Shanjie , Yu Junshen , Guo Xinjuan , Hou Xiaojie , Xu Jin , Li Sen , Xing Guoming 
Published: October 2024

IntroductionNAC (NAM, ATAF, and CUC) transcription factor family, one of the important switches of transcription networks in plants, functions in plant growth, development, and stress resistance. Night lily (Hemerocallis citrina) is an important horticultural perennial monocot plant that has edible, medicinal, and ornamental values. However, the NAC gene family of night lily has not yet been analyzed systematically to date.MethodsTherefore, we conducted a genome-wide study of the HcNAC gene family and identified a total of 113 HcNAC members from the Hemerocallis citrina genome.ResultsWe found that 113 HcNAC genes were unevenly distributed on 11 chromosomes. Phylogenetic analysis showed that they could be categorized into 16 instinct subgroups. Proteins clustering together exhibited similar conserved motifs and intron–exon structures. Collinearity analysis indicated that segmental and tandem duplication might contribute to the great expansion of the NAC gene family in night lily, whose relationship was closer with rice than Arabidopsis. Additionally, tissue-specific pattern analysis indicated that most HcNAC genes had relatively higher expression abundances in roots. RNA-Seq along with RT-qPCR results jointly showed HcNAC genes expressed differently under drought and salinity stresses. Interestingly, HcNAC35 was overexpressed in watermelon, and the stress resilience of transgenic lines was much higher than that of wild-type watermelon, which revealed its wide participation in abiotic stress response.ConclusionIn conclusion, our findings provide a new prospect for investigating the biological roles of NAC genes in night lily.
Effects of activity participation and cognitive levels on depression in middle-aged and older adults with chronic illness: a national cross-sectional study
Authors: Jin Lei , Jing Feiyue 
Published: October 2024

IntroductionThe world population is rapidly aging, and depression mainly affects middle-aged and older adults with chronic diseases and cognitive impairments. The sample for this study was obtained from the China Health and Retirement Longitudinal Study (CHARLS) public database. The sample size for inclusion was 12,767. There were 6,773 females and 5,994 males, with an overall low level of education. This study aims to provide a theoretical and practical reference basis for the clinical non-pharmacological treatment of depression in middle-aged and older adults (age ≥ 50 years) with chronic diseases. Additionally, the study seeks to promote the development of mental health interventions for middle-aged and older adults (age ≥ 50 years) with chronic diseases, ultimately enhancing the sense of well-being and quality of life for this demographic.MethodsCognitive functioning and depressive symptoms of the study participants were assessed using the Mini-Mental State Examination Scale (MMSE) and the short version of the Center for Epidemiological Studies Depression Scale (CESD-10).Results and discussionBinary logistic regression results showed that among middle-aged and older adults (age ≥ 50 years) with chronic diseases, participation in physical activity [OR = 1.397; 95% CI (1.181–1.651); p &lt; 0.05] was more effective than participation in social activities [OR = 0.997; 95% CI (0.924–1.076); p &lt; 0.05] for preventing depression. Those with cognitive impairment [OR = 1.206; 95% CI (1.089–1.335); p &lt; 0.05] were more likely to experience depression than those without cognitive impairment. Activity participation (physical activity and social activity) had a more significant effect on mild and moderate depression compared to no depression, and cognitive level had a more pronounced effect on moderate depression [OR = 1.491; 95% CI (1.278–1.740); p &lt; 0.05] and major depression [OR = 2.231; 95% CI (1.282–3.884); p &lt; 0.05]. Within the specific cohort of middle-aged and older adults (age ≥ 50 years) with chronic diseases, both activity participation and cognitive level exert a significant influence on the prevention and intervention of depression. Engagement in physical activity, participation in social activities, and enhanced cognitive functioning emerged as protective factors against depression. Therefore, the policy-maker should strengthen the prevention and treatment of depression in a comprehensive manner through the promotion of physical and social activities and the enhancement of cognitive level, so as to safeguard the mental health of middle-aged and older adults with chronic diseases.
Do emotional demands and exhaustion affect work engagement? The mediating role of mindfulness
Authors: Karahan Kaplan Merve , Bozkurt Gözde , Aksu Bumin Çağatay , Bozkurt Serdar , Günsel Ayşe , Gencer Çelik Gülşah 
Published: October 2024

AimThe current paper seeks to elucidate the interrelationships among emotional demands (ED), emotional exhaustion (EE), mindfulness, and work engagement (WE), with an explanation of the mediating role of mindfulness within indicated relationships.BackgroundNurses working in a stress-related environment face some emotional challenges. New methods such as mindfulness should be learned; therefore, positive outcomes occur along with new developments.MethodResponses were received from nurses through a self-report questionnaire using the convenience sample technique. Four hundred and twenty-nine nurses from health institutions in Istanbul participated in the study. The PLS-SEM technique was used to test the research model.ResultsED and mindfulness, EE and mindfulness, and mindfulness and WE relationships were found. While mindfulness was a mediator between EE and WE, it was not a mediator between ED and WE.ConclusionIt has been revealed that nurses cope with job-related challenges by being present at the moment with high awareness. Furthermore, mindful nurses also foster positive outcomes.Implications for nursing managementPrecautions should be taken because of the nursing shortage. It is better to provide mindfulness training to nursing students in schools before their professional lives. Implementing and using technologies might be helpful for nurses.
The impact of sleep disturbances on treatment efficacy and prognosis in patients with depressive and anxiety disorders
Authors: Zhang Qingyu , Tong Maoqing , Ji Yunxin , Hou Yanbin , Lou Zongze , Wu Danjuan , Mi Yuwei , Miu Pingping , Tian Jiaxin , Zhu Zhenzhen , Ruan Liemin 
Published: October 2024

IntroductionLittle was known about the relationship between sleep disturbances and depressive and anxiety disorders, as well as the efficacy of treatment regimens.MethodsDuring 2021 to 2023, a total of 417 participants were screened by Hamilton Depression Rating Scale (HAMD-17) and Hamilton Anxiety Rating Scale (HAMA-14) for psychological status, and Pittsburgh sleep quality index (PSQI) assessment. 409 participants were finally enrolled, of which 188 (45.97%) were suffered from sleep disorders. All participants were received polysomnography (PSG) followed by six-week pharmacological treatment of escitalopram and zopiclone, and finally assessed by HAMD-17 and HAMA-14 for treatment efficacy.ResultPSG monitoring indicated that participants with depression experienced prolonged rapid eye movement sleep latency (REMSL) and increased wakefulness after sleep onset (WASO) (P=0.030 and P=0.002, respectively). Those with anxiety disorders demonstrated a significantly higher percentage of non-rapid eye movement sleep (NREM%) and reduced WASO (P=0.013 and P=0.001, respectively). After six-weeks pharmacological treatment, participants with or without sleep disorders exhibited with similar efficacy outcomes of depression and anxiety disorders (P&gt;0.05). However, every point of PSQI increment at baseline would decrease 0.78 and 0.85 times of probability of effective pharmacological treatment of depression and anxiety disorders. Moreover, participants with both effective outcomes of depression and anxiety disorders were found significant shorter sleep onset latency (SOL) (P&lt;0.001).DiscussionThe insights gained underscore the necessity of considering sleep disturbances in enhancing the overall effectiveness of pharmacological treatments for depression and anxiety disorders.
Parental emotional support, self-efficacy, and mental health problems among adolescents in Hong Kong: a moderated mediation approach
Authors: Qian Mengting , Jin Rui , Lu Chunping , Zhao Mingren 
Published: October 2024

IntroductionEarly signs of mental health issues may develop into severe mental illnesses over time. The influence of parental emotional support on adolescent mental health problems is well acknowledged. However, prior research has predominantly focused on psychological symptoms, often neglecting the somatic symptoms associated with mental health. Additionally, there has been insufficient exploration of the mediating role of self-efficacy and the relationship between socioeconomic status (SES) and adolescent mental health, particularly within the context of Hong Kong.MethodsUsing data from 3,613 adolescents aged 15 years from 109 schools in Hong Kong who participated in the Programme for International Student Assessment 2018, this study explored the relationship between parental emotional support and two dimensions of adolescent mental health symptoms (psychological and somatic symptoms), and the mediating effect of self-efficacy and the moderating role of SES.ResultsWe found that parental emotional support significantly reduced psychological and somatic symptoms, primarily by enhancing self-efficacy. MoreovSES moderated these relationships, with the impact of parental emotional support on psychological symptoms being more pronounced among adolescents from lower SES backgrounds.DiscussionThis study deepens the understanding of the mechanisms underlying adolescent mental health in Hong Kong. By highlighting the importance of parental emotional support and self-efficacy, as well as the moderating effect of SES, the findings offer valuable insights for effective interventions aimed at improving adolescent mental health.
Adherence to infection prevention practices and associated factors among healthcare workers in Northeastern Ethiopia, following the Northern Ethiopia conflict
Authors: Berihun Gete , Gizeyiatu Adinew , Berhanu Leykun , Sewunet Birhanu , Ambaw Birhanie , Walle Zebader , Abebe Masresha , Gasheya Kassahun Ayele 
Published: October 2024

BackgroundIn resource-limited areas, especially in conflict-affected settings, managing the risk of hospital-acquired infections is difficult due to the destruction of essential facilities in healthcare settings. The study aimed to assess adherence to Infection prevention practices and associated factors among healthcare workers in Northeastern Ethiopia following the Northern Ethiopia conflict.Methods and materialsA facility-based cross-sectional study was done with 408 healthcare workers. The survey data was collected using a structured questionnaire based on published articles. Data entry and analysis was done using Epi-Data version 4.6 and SPSS version 25.0, respectively. Binary logistic regression was used to determine the association between dependent and outcome variables, with a cut-off p value 0.05 at 95% confidence interval with a p-value less than 0.05 and a 95% confidence interval for determining factors associated with adherence to infection prevention practices among study participants.ResultsThe study included 408 healthcare workers with a response rate of 96.7%. The majority of participants were women 206 (50.5%), married 250 (61.3%), Orthodox followers 211 (51.7%), and educational status of master holder and above with a master’s degree or higher 177 (43.4%). Slightly more than half, 53.7% (219) of the respondents demonstrated safe infection prevention practices. Respondents who received training on infection prevention practices (AOR = 2.662, 95% CI: 1.361, 5.120) had an active infection prevention committee (AOR = 2.203, 95% CI: 1.359, 3.572), use infection prevention guidelines in working departments (AOR = 2.090, 95% CI: 1.013, 4.312), and access to adequate personal protective equipment (AOR = 2.773, 95% CI: 1.560, 4.929) were factors significantly associated with adherence to infection prevention practices.ConclusionOverall, only half of the respondents practiced safe infection prevention practices. Receive training on infection prevention guidelines, presence of active infection prevention committee and working guidelines, and the availability of personal protective equipment were factors of infection prevention. Hence, essential facilities like Personal protective equipment, working guidelines should be supplied by donors.
Cost analysis of technological vs. conventional upper limb rehabilitation for patients with neurological disorders: an Italian real-world data case study
Authors: Gower Valerio , Aprile Irene , Falchini Francesca , Fasano Alessio , Germanotta Marco , Randazzo Mattia , Spinelli Federico , Trieste Leopoldo , Gramatica Furio , Turchetti Giuseppe 
Published: October 2024

IntroductionMost patients suffering from neurological disorders endure varying degrees of upper limb dysfunction, limiting their everyday activities, with only a limited number regaining full arm use. Robotic and technological rehabilitation has been demonstrated to be a feasible solution to guarantee an effective rehabilitation to recover upper limb performance or to prevent complications of upper limb immobility. However, there is currently a lack of studies which analyze the sustainability of robotic and technological rehabilitation by comparing its costs to conventional rehabilitation pathways.MethodsSince technology-based and conventional rehabilitation of the upper limb have been demonstrated to have comparable efficacy when the rehabilitation dose is matched, our study concentrates on a cost minimization analysis. The aim of the study is to compare the costs of a “mixed” rehabilitation cycle, which combines conventional and technology-based treatments (the latter delivered with a single therapist supervising several patients), with a cycle of purely conventional treatments. This has been done by developing a cost model and retrospectively analyzing the costs sustained by an Italian hospital which has adopted such a mixed model. A sensitivity analysis has been done to identify the parameters of the model that have the greatest influence on cost difference and to evaluate their optimal values in terms of efficiency of mixed rehabilitation. Finally, probabilistic simulations have been applied to consider the variability of model parameters around such optimized values and evaluate the probability of achieving a given level of savings.ResultsWe found a cost difference of 49.60 € per cycle in favor of mixed rehabilitation. The sensitivity analysis demonstrated that, in the situation of the hospital under investigation, the parameter having the largest influence on the cost difference is the number of robotic treatments in a mixed rehab cycle. Probabilistic simulations indicate a probability higher than 98% of an optimized mixed rehabilitation cycle being less expensive than a pure conventional one.ConclusionThrough a retrospective cost analysis, we found that the technology-based mixed rehabilitation approach, within a specific organizational model allowing a single physiotherapist to supervise up to four patients concurrently, allowed cost savings compared to the conventional rehabilitation model.
Analysis of multi-site HPV infection and vaccination willingness among men who have sex with men in Tianjin, China
Authors: Bai Jianyun , Dong Xiaoyue , Ning Tielin , Zhu Jingjin , Wu Ziming , Li Huijuan , Yu Maohe 
Published: October 2024

BackgroundMen who have sex with men (MSM) are vulnerable to HPV infection. This study aims to explore the HPV infection status at different sites among HIV-positive MSM, HIV-negative MSM, and men who have sex with women (MSW), and to investigate their willingness to receive HPV vaccination.MethodsFrom September 2023 to April 2024, three groups were recruited in Tianjin, China. Participants completed an electronic self-administered questionnaire, which included demographic information, knowledge related to sexually transmitted diseases, behavioral information, and willingness to receive the HPV vaccine. Samples were collected from the anal region, genitals, and oral cavity for HPV typing.ResultsA total of 1,559 participants were recruited, including 300 HIV-positive MSM, 600 HIV-negative MSM, and 659 MSW. The HPV infection prevalence for any site were 62.0, 53.7 and 8.3%, respectively (p &lt; 0.001). The infection prevalence for HPV genes covered by the 9-valent vaccine were 47.0, 36.8, and 3.5%, respectively (p &lt; 0.001). Co-infection prevalence at anal and genital were 20.3, 14.2, 0.6%, respectively. Co-infection prevalence at anal and genital and oral were 1.3, 0.3%, 0, respectively. A total of 77.0% HIV-positive MSM and 75.3% HIV-negative MSM expressed willingness to receive the HPV vaccine, whereas 58.9% of MSW were unwilling (p &lt; 0.001). Being HIV-positive (aOR, 3.119; 95% CI, 2.213–4.395), being over 46 years old (aOR, 1.994; 95% CI, 1.266–3.142), with an occupation classified as “white collar workers” (aOR, 1.620; 95% CI, 1.111–2.362) and “freelancing” (aOR, 2.025; 95% CI, 1.371–2.993) and a history of homosexual behavior in the past 6 months (aOR, 5.338; 95% CI, 3.802–7.495) were risk factors for HPV infection among men in Tianjin. Consistently using condoms in the past 6 months (aOR, 0.667; 95% CI, 0.513–0.867) were protective factors.ConclusionThe HPV infection prevalence among MSM in Tianjin is significantly higher than among MSW, with higher prevalence in the anal region compared to the genital and oral region. HPV infection is associated with HIV infection, older age, and homosexual behavior. Most MSM showed a positive willingness to receive the HPV vaccine, indicating the necessity to implement targeted HPV vaccination programs for MSM and to enhance necessary preventive knowledge and behavioral interventions.
Acute effect of resistive aquatic high-intensity interval training on metabolic costs in adults
Authors: Kwok Manny M. Y. , Ng Shamay S. M. , Ng Y. M. , Tan Gordon C. C. , Huang P. P. , Zhang Y. , So Billy C. L. 
Published: October 2024

BackgroundThe effects of Aquatic High-Intensity Interval Training (AHIIT) and resistive AHIIT (Resistive AHIIT) to improve metabolic responses were not yet known.ObjectiveThis study was to compare the metabolic responses and perceived effort in young healthy adults in a single session of AHIIT and resistive AHIIT.Methods20 healthy subjects (9 females, 11 males) performed a stationary running at a matched exercise intensity prior AHIIT and resistive AHIIT [10 × 1-min bouts of stationary running at 90% maximum heart rate (HR max) separated by 1-min active recovery] to examine the metabolic and cardiometabolic outcomes. Mixed effects models were applied to analyze the effects of group, time, and the interaction between group and time on both outcomes. The level of correlations between metabolic variables was checked by Pearson's linear correlation.ResultsThere are significant differences on pre and post resting energy expenditure (REE) within both AHIIT and resistive AHIIT groups (p &lt; 0.01) respectively as well as the subjective rate of perceived exertion (RPE) (p &lt; 0.01) within RAHIIT group. A moderate correlation found on respiratory exertional ratio (RER) and RPE in resistive AHIIT (r = 0.534). No significant differences between groups in terms of HR max, mean heart rate (HR mean), peak oxygen consumption (VO2 peak) and total energy expenditure (TEE) (p = 0.50, p = 0.48, p = 0.81, p = 0.59).ConclusionResistive AHIIT provides comparable benefits of metabolic outcomes with AHIIT. Comparable results allowed AHIIT and resistive AHIIT prescriptions precisely.
Tacrolimus Dose Requirement in De Novo Adult Kidney Transplant Patients Treated With Adoport® Can Be Anticipated
Authors: Marquet Pierre , Anglicheau Dany , Humeau Antoine , Adrouche Sofian , Saada Lakhdar , Bisiaux Julie , Guillemin Sara , Lardy-Cléaud Audrey , Rostaing Lionel 
Published: October 2024

All the factors potentially influencing tacrolimus dose requirement and combinations thereof have never been thoroughly investigated, precluding accurate prediction of tacrolimus starting dose. This prospective, non-interventional, multicenter study in de novo adult kidney transplant recipients over the first year after transplantation aimed to investigate the factors influencing tacrolimus dose-standardized trough blood concentration (C0/D) over the first week post-transplant (D4-D7, primary objective), D8-M3 and M3-M12 (secondary objectives). Statistical analysis employed mixed linear models with repeated measures. Eighteen sites enrolled 440 patients and followed them up for 9.5 ± 4.1 months. Age at baseline (p = 0.0144), end-stage renal disease (p = 0.0092), CYP3A phenotype (p &lt; 0.0001), dyslipidemia at baseline (p = 0.0031), hematocrit (p = 0.0026), total bilirubin (p = 0.0261) and plasma creatinine (p = 0.0484) independently increased with log(C0/D) over D4-D7, explaining together 72.3% of the interindividual variability, and representing a robust model to estimate tacrolimus initial dose. Donor age and CYP3A phenotype were also influential over D8-M3 and M3-12, in addition to recipient age. Corticosteroids, diabetes at baseline, and ASAT yielded inconstant results between D8-M3 and M3-M12. We found no ethnicity effect when CYP3A phenotype was accounted for, and no food effect. Intra-individual variability over M3-M12 was moderate, and significantly lower in patients with chronic hepatic disorder (p = 0.0196) or cancer (p = 0.0132).