bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanistic insights into the inhibition of Sec61-dependent co- and post-translational translocation by mycolactone
Authors: McKenna Michael , Simmonds Rachel E. , High Stephen 
Published: February 2016

ABSTRACT
The virulence factor mycolactone is responsible for the immunosuppression and tissue necrosis that characterise Buruli ulcer, a disease caused by infection with Mycobacterium ulcerans. In this study, we confirm that Sec61, the protein-conducting channel that coordinates entry of secretory proteins into the endoplasmic reticulum, is a primary target of mycolactone, and characterise the nature of its inhibitory effect. We conclude that mycolactone constrains the ribosome–nascent-chain–Sec61 complex, consistent with its broad-ranging perturbation of the co-translational translocation of classical secretory proteins. In contrast, the effect of mycolactone on the post-translational ribosome-independent translocation of short secretory proteins through the Sec61 complex is dependent on both signal sequence hydrophobicity and the translocation competence of the mature domain. Changes to protease sensitivity strongly suggest that mycolactone acts by inducing a conformational change in the pore-forming Sec61α subunit. These findings establish that mycolactone inhibits Sec61-mediated protein translocation and highlight differences between the co- and post-translational routes that the Sec61 complex mediates. We propose that mycolactone also provides a useful tool for further delineating the molecular mechanisms of Sec61-dependent protein translocation.
EEG correlates of social interaction at distance
Authors: Giroldini William , Pederzoli Luciano , Bilucaglia Marco , Caini Patrizio , Ferrini Alessandro , Melloni Simone , Prati Elena , Tressoldi Patrizio E. 
Published: February 2016

This study investigated EEG correlates of social interaction at distance between twenty-five pairs of participants who were not connected by any traditional channels of communication.Each session involved the application of 128 stimulations separated by intervals of random duration ranging from 4 to 6 seconds. One of the pair received a one-second stimulation from a light signal produced by an arrangement of red LEDs, and a simultaneous 500 Hz sinusoidal audio signal of the same length. The other member of the pair sat in an isolated sound-proof room, such that any sensory interaction between the pair was impossible.An analysis of the Event-Related Potentials associated with sensory stimulation using traditional averaging methods showed a distinct peak at approximately 300 ms, but only in the EEG activity of subjects who were directly stimulated. However, when a new algorithm was applied to the EEG activity based on the correlation between signals from all active electrodes, a weak but robust response was also detected in the EEG activity of the passive member of the pair, particularly within 9 – 10 Hz in the Alpha range. Using the Bootstrap method and the Monte Carlo emulation, this signal was found to be statistically significant.
Mathematical modeling of processes in biotechnical complex «corrective action facilities – humax respiratory system»
Authors: Минаков  , Minakov E. , Федоров  , Fedorov S. , Ивахно  , Ivakhno N. , Анцибор  , Antsibor S. 
Published: February 2016

The article considers the aim of modeling humax respiratory system structure for predicting the
change of physiological parameters at different methods of physical influence such as operated reduction in
breathing circuit. The developed complex of mathematical models includes: equations of mass rate and masstransfer
rate in lung channels at arborization of auriferous ways according to the rule of right dichotomy; equations
of breathing muscles dynamics, based on moving of cylinder wall in radial and axial directions; special
functions, providing automatic muscles turning on and turning off when lungs reach minimal and maximal set
volume at inhale/exhale.
The article shows the results of numerical modeling of functioning of the sophisticated biotechnical complex
«Corrective action facilities – humax respiratory system» in the form of pressure charts and volume flow
which the lungs generate in different regimes of resistance in breathing circuit. The results acquired qualitatively
and quantitatively reflect the biomechanics of the number of processes which accompany breathing.
The developed complex of interconnected mathematical models allows to fulfill specific multiparametric
modeling of the dynamics of functioning of the complex biotechnical system «Corrective action facilities – humax
respiratory system», which allows the realization of optimal controlling algorithms for execution units in
different complexes based on prediction of changes in the condition of the respiratory system.
Efficiency of correction of autonomic disorders in the conditions of meteo-climatic maladaptation by the methods of reflex and crystal therapy
Authors: Михайлова  , Mikhaylova A. , Круглянин  , Kruglyanin K. , Файзуллоев  , Fayzulloev A. 
Published: February 2016

The change of climatic conditions often leads to stress and sometimes failure of adaptive resources.
Vegetative nervous system is one of the main participants of adaptation to environmental changes. Its
functions inevitably decline in cases of short-term change of meteorological and climatic conditions, especially,
in people with history of vascular dystonia. The article presents the results of correction of the autonomic function
by the methods of reflex- and crystal therapy in the conditions of maladaptation due to meteo-climatic
changes. Due to its popularization in world medical practice and absence of research within evidence-based
medicine, non-drug therapy, used in complementary medicine, in particular crystal therapy, require evaluation of
its effectiveness for correction of autonomic disorders in cases of maladaptation and comparing with the generally
accepted method of reflexology. Crystal therapy is a method of health improvement, which involves applying
precious and semiprecious minerals to various parts of the body. Assessment of vegetative nervous system in
healthy men and women with the diagnosis of vascular dystonia was carried out on the first day and 7-8 days
after the arrival to Anapa. Randomized, blind, placebo controlled study has shown significant difference of the
effect of reflex- and crystal therapy from the control group and the placebo group, in the correction of vegetative
disturbances according to the heart rate variability and a number of mental health indicators. It has proven the
effectiveness of reflexotherapy in the case of a high degree of maladaptation and crystal therapy in case of mild
to moderate severity of maladaptation, identified according to the coefficient of weather variability in traffic
along various routes.
Gender differences in schizophrenia
Authors: Бобровникова  , Bobrovnikova A , Тарасова  , Tarasova S. 
Published: February 2016

The study involved 32 patients with schizophrenia (F20). The main criterion of the analysis of
gender characteristics is the trigger of the disease development. The authors analyze the possible role of psychological
factors of traumatization in the interpretation of the mechanisms of formation and development of some
symptoms of mental disorders in schizophrenics. According to the study, the trigger of the disease in women
often has been the traumatic situation connected with unsuccessful romantic and sexual experiences: rape, attempted
rape, &#34;unrequited love&#34; (56% of cases). Among the factors aggravating premorbid background in women,
the authors noted the disturbances of eating behavior (37%) and suicidal behaviour (31% of cases). In men,
the trigger is associated with trauma in the field of interpersonal relations (38% of cases). As aggravating factors
in men, the authors identified anti-social behaviour (18%) and the use of alcohol and drugs (50% of cases). The
analysis of socio-psychological factors of the disease revealed considerable social in women. Similar results
were obtained the following factors: the criticality of the patient to his state and the safety of the patient in selforientation.
56% women are relatively critical to its defective state, this parameter in men is 37%.
Estimation of parametres of cardiovascular systems in students of the surgut state university
Authors: Кузнецова  , Kuznetsova V. , Ворошилова  , Voroshilova O. , Соколова  , Sokolova A. , Тен  , Ten R. 
Published: February 2016

It is known that the health of people living in the North is often very different from the norm.
The research was conducted in the winter. The parameters of the autonomic nervous system and the bioelectrical
activity of the heart are studied. To achieve results, the authors used cardiorhythmography examination and standard
method of pulse oximetry. The parameters of the autonomic nervous system activity and the bioelectric
activity of the heart are investigated. Synergetic analysis of parameters of quasi-attractor motion of the state vector
of the organism students showed that the organism of young men is in a relatively unstable state to extreme
climate and environmental conditions of the North. As a result, boys have a greater risk of developing early heart
disease. The parameters of quasi-attractors of vector states of the body in boys (according to the indicators of the
autonomic nervous system) are confirmed by the results of the analysis of the parameters of quasi-attractor behavior
of another vector of states, characterizing bioelectrical activity of the heart.
The sanogenesis from cell positions
Authors: Наумова  , Naumova E. , Кидалов  , Kidalov V. , Фудин  , Fudin N. , Валентинов  , Valentinov B. 
Published: February 2016

The review highlights the issues of formation and development of the sanogenesis, the classification
of sanogenetic mechanisms, theirs role in rehabilitation. The interpenetration of sanogenetic and pathogenetic
mechanisms, participation of different tissues in the sanogenesis, including blood, is defined. It is showed
the structure and significance of the erythron, formation of red blood cells, building border line of red blood
cells, the significance of autofluorescence in the evaluation of sanogenetic reactions. The energetic importance
of mitochondria, the receptor apparatus, various enzymes, transport proteins, electron transport chain in the metabolism
that determines the degree of loss of sanogenetic potential and formation of preexisting diseases is defined.
It is established the role of sanogenic reactions in the prevention of stress and post-stress recovery.
Analyzes of Cold Accumulation
Authors: Синцов  , Sintsov A. 
Published: February 2016

The aim of this article is to identify opportunities of energy saving during the production of cold. Analysis of the cold
accumulation in refrigeration units with variable refrigeration load was carried out in order to reduce energy consumption.
Schemes of ice-water and air conditioning installations, which use ice accumulators, are presented. Varieties of ice
accumulator cycles and calculation results are provided. Reduced cooling efficiency of the two-stage versus single-stage
cooling is confirmed. The possibility of replacing ice accumulator with seasonal cold accumulator is considered. A onedimensional
mathematical model of ice buildup in ice accumulator is offered. The calculation results are compared with
the characteristics of actual products. Energy saving by replacing ice accumulators with seasonal cold accumulators is
confirmed by the results of calculations.
Fake It Till You Make It: Reputation, Competition, and Yelp Review Fraud
Authors: Luca Michael , Zervas Georgios 
Published: February 2016

Consumer reviews are now part of everyday decision making. Yet the credibility of these reviews is fundamentally undermined when businesses commit review fraud, creating fake reviews for themselves or their competitors. We investigate the economic incentives to commit review fraud on the popular review platform Yelp, using two complementary approaches and data sets. We begin by analyzing restaurant reviews that are identified by Yelp’s filtering algorithm as suspicious, or fake—and treat these as a proxy for review fraud (an assumption we provide evidence for). We present four main findings. First, roughly 16% of restaurant reviews on Yelp are filtered. These reviews tend to be more extreme (favorable or unfavorable) than other reviews, and the prevalence of suspicious reviews has grown significantly over time. Second, a restaurant is more likely to commit review fraud when its reputation is weak, i.e., when it has few reviews or it has recently received bad reviews. Third, chain restaurants—which benefit less from Yelp—are also less likely to commit review fraud. Fourth, when restaurants face increased competition, they become more likely to receive unfavorable fake reviews. Using a separate data set, we analyze businesses that were caught soliciting fake reviews through a sting conducted by Yelp. These data support our main results and shed further light on the economic incentives behind a business’s decision to leave fake reviews. This paper was accepted by Lorin Hitt, information systems.
Corporate Postretirement Benefit Plans and Real Investment
Authors: Bartram Söhnke M. 
Published: February 2016

This paper shows that the real investment by nonfinancial firms is systematically related to the size of their defined-benefit plan. In particular, these plans allow research and development (R&D)-intensive firms to retain and borrow from their employees, which is attractive since they have high adjustment costs, require a stable cash flow, have less collateral, have assets that are harder to value, and have high asymmetric information and agency costs. By contrast, capital expenditures benefit from credit multiplier effects with regard to regular debt, which substitutes for postretirement obligations. As a result, firms with defined-benefit plans have 12% more R&D and 5% less capital expenditure compared with otherwise similar firms, and they vary contributions as a function of cash flow and real investment. The role of postretirement plans is attenuated in countries with available alternative funding sources. The results are robust to controlling for other dimensions of financial policy—cash holdings, debt maturity, dividends, preferred stock, convertible debt, and leverage—that also affect real investment. This paper was accepted by Gustavo Manso, finance.
High tibial osteotomy in knee laxities: Concepts review and results
Authors: Robin Jonathan G. , Neyret Philippe 
Published: February 2016

Patients with unstable, malaligned knees often present a challenging management scenario, and careful attention must be paid to the clinical history and examination to determine the priorities of treatment. Isolated knee instability treated with ligament reconstruction and isolated knee malalignment treated with periarticular osteotomy have both been well studied in the past. More recently, the effects of high tibial osteotomy on knee instability have been studied. Lateral closing-wedge high tibial osteotomy tends to reduce the posterior tibial slope, which has a stabilising effect on anterior tibial instability that occurs with ACL deficiency. Medial opening-wedge high tibial osteotomy tends to increase the posterior tibia slope, which has a stabilising effect in posterior tibial instability that occurs with PCL deficiency. Overall results from recent studies indicate that there is a role for combined ligament reconstruction and periarticular knee osteotomy. The use of high tibial osteotomy has been able to extend the indication for ligament reconstruction which, when combined, may ultimately halt the evolution of arthritis and preserve their natural knee joint for a longer period of time. Cite this article: Robin JG, Neyret P. High tibial osteotomy in knee laxities: Concepts review and results. EFORT Open Rev 2016;1:3-11. doi: 10.1302/2058-5241.1.000001.
Journalism education in China: The reality and challenges in the digital era
Authors: Long Yun , Zeng Xin 
Published: February 2016

Abstract
With the rise of digital technologies and the opening-up in society, China’s journalism education has entered a period of fast growth. While the media environment is adapting to the changes, journalism education is still developing in a traditional manner, thus failing to provide up-to-date teaching for students. Meanwhile, because of gaps between academics and professionals, graduates are not appreciated by media industries. On the basis of the background of media development and social reality in China, the article endeavours to elaborate on issues and challenges of Chinese journalism education by revealing the relationship between policy, media and journalism education. The article suggests that the problems in journalism education are caused by the social system rather than by education. Therefore, instead of focusing on issues invited by the new technology, market or the conflict between the academic and professionals, it is more crucial for Chinese journalism education to face the challenge caused by the social system, balancing between policy and professionalism, in order to find a way for journalism education to develop in a Chinese context.
Increased expression and secretion of recombinant hIFNγ through amino acid starvation-induced selective pressure on the adjacent HIS4 gene in Pichia pastoris
Authors: Razaghi Ali , Huerlimann Roger , Owens Leigh , Heimann Kirsten 
Published: February 2016

Abstract
Transcriptional co-regulation of adjacent genes has been observed for prokaryotic and eukaryotic organisms, alike. High levels of gene adjacency were also found in a wide variety of yeast species with a high frequency of co-regulated gene sets. The aim of this research was to study how selective pressure on the Histidinol dehydrogenase gene (HIS4), using amino acid starvation, affects the level of expression and secretion of the adjacent human interferon gamma gene (hIFNγ) in the recombinant Pichia pastoris GS115 strain, a histidine-deficient mutant. hIFNγ was cloned into the pPIC9 vector adjacent to the HIS4 gene, a gene essential for histidine biosynthesis, which was then transformed into P. pastoris. The transformed P. pastoris was cultured under continuous amino acid starvation in amino acid-free minimal medium for ten days, with five inoculations into unspent medium every second day. Under these conditions, only successfully transformed cells (hIFNγ -HIS4+) are able to synthesise histidine and therefore thrive. As shown by ELISA, amino acid starvation-induced selective pressure on HIS4 improved expression and secretion of the adjacent hIFNγ by 55% compared to unchallenged cells. RT-qPCR showed that there was also a positive correlation between duration of amino acid starvation and increased levels of the hIFNγ RNA transcripts. According to these results, it is suggested that these adjacent genes (hIFNγ and HIS4) in the transformed P. pastoris are transcriptionally co-regulated and their expression is synchronised. To the best of the knowledge of the authors; this is the first study demonstrating that amino acid starvationinduced selective pressure on HIS4 can alter the regulation pattern of adjacent genes in P. pastoris.
Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue
Authors: Tero-Vescan Amelia , Osz Bianca-Eugenia , Vari Camil-Eugen , Imre Silvia , Hancu Gabriel 
Published: February 2016

Abstract
Objectives: The purpose of the study was to determine the omega-3/omega-6 ratio in swine brain homogenate by HPLC with UV detection and to discuss the values obtained by comparison to the human species. Materials and methods: Determinations were performed by HPLC method using as mobile phase an isocratic mixture (A:B - 5:95) of mobile phase A = 25% acetonitrile in water and B = acetonitrile with a flow-rate of 1.2 mL/min and UV detection at 205nm. Chromatographic column: Phenomenex C8 150x4.6 mm 5μm. 50 g swine brain was hydrolyzed with 100 mL 0.5N HCl, the organic phase was extracted in 50 mL hexane, concentrated by evaporation and resumed in 200 μL acetonitrile. Results: Polyunsaturated fatty acids were separated as follows arachidonic acid (AA) - Rt = 2.69 min, docosahexaenoic acid (DHA) - Rt = 3.12 min and eicosapentaenoic acid (EPA) - Rt = 3.97 min. The following omega-3/omega-6 ratios were calculated (DHA + EPA)/ AA = 0.572 ± 0.451, EPA/AA = 0.027 ± 0.015 and DHA/AA = 0.689 ± 0.612. Conclusions: The values obtained for these ratios should be balanced, but in reality they are in favor of the ratio denominator. Considering the physiological and nutritional similarities and that an accurate diagnosis of neurodegenerative disease is set in post-mortem, swine brain homogenate could serve as an animal model for human nervous tissue.
Internal Structure Quality Control of Solid Pharmaceuticals. A Comparative Study
Authors: Imre Silvia , Haidu Timea , Ponta Oana , Vancea Szende , Vari Camil-Eugen , Tero-Vescan Amelia 
Published: February 2016

Abstract
Objective: The aim of the study was a comparative investigation by spectral and thermal analysis in order to asses a number of characteristics of different varieties ofrawmaterials of ursodeoxycholic acid and ibuprofen. The different dissolution behavior of two ursodeoxycholic acid pharmaceutical product by crystallinity pattern was investigated. Methods: Raw materials of ursodeoxycholic acid and ibuprofen were used. IR spectroscopy, differential scanning calorimetry and X-Ray Diffraction Analysis were applied. Results: The results show no crystallinitydifferences for different batches of the tested drugs. No solid solid transition was proved during sample preparation for transmission IR analysis. Conclusions: A combination of two more affordabletests by IR spectrometry and differential scanning calorimetry lead to the same results as X-Ray diffraction analysis for crystallinity similarity assessment of the studied substances. The dissolution differences of test drugs were not related to the polymorphism of the raw materials.
Snake Venom Metalloproteinases
Authors: Gâz Florea Şerban Andrei , Gâz Florea Adriana , Kelemen Hajnal , Muntean Daniela-Lucia 
Published: February 2016

Abstract
As more data are generated from proteome and transcriptome analysis revealing that metalloproteinases represent most of the Viperid and Colubrid venom components authors decided to describe in a short review a classification and some of the multiple activities of snake venom metalloproteinases. SVMPs are classified in three major classes (P-I, P-II and P-III classes) based on the presence of various domain structures and according to their domain organization. Furthermore, P-II and P-III classes were separated in subclasses based on distinctive post-translational modifications. SVMPs are synthesized in a latent form, being activated through a Cys-switch mechanism similar to matrix metalloproteinases. Most of the metalloproteinases of the snake venom are responsible for the hemorrhagic events but also have fibrinogenolytic activity, poses apoptotic activity, activate blood coagulation factor II and X, inhibit platelet aggregation, demonstrating that SVMPs have multiple functions in addition to well-known hemorrhagic function.