bluuebunny/crossref_metadata_2025_split huggingface dataset:
A microengineered model of RBC transfusion-induced pulmonary vascular injury
Authors: Seo Jeongyun , Conegliano David , Farrell Megan , Cho Minseon , Ding Xueting , Seykora Thomas , Qing Danielle , Mangalmurti Nilam S. , Huh Dongeun 
Published: June 2017

AbstractRed blood cell (RBC) transfusion poses significant risks to critically ill patients by increasing their susceptibility to acute respiratory distress syndrome. While the underlying mechanisms of this life-threatening syndrome remain elusive, studies suggest that RBC-induced microvascular injury in the distal lung plays a central role in the development of lung injury following blood transfusion. Here we present a novel microengineering strategy to model and investigate this key disease process. Specifically, we created a microdevice for culturing primary human lung endothelial cells under physiological flow conditions to recapitulate the morphology and hemodynamic environment of the pulmonary microvascular endothelium in vivo. Perfusion of the microengineered vessel with human RBCs resulted in abnormal cytoskeletal rearrangement and release of intracellular molecules associated with regulated necrotic cell death, replicating the characteristics of acute endothelial injury in transfused lungs in vivo. Our data also revealed the significant effect of hemodynamic shear stress on RBC-induced microvascular injury. Furthermore, we integrated the microfluidic endothelium with a computer-controlled mechanical stretching system to show that breathing-induced physiological deformation of the pulmonary microvasculature may exacerbate vascular injury during RBC transfusion. Our biomimetic microsystem provides an enabling platform to mechanistically study transfusion-associated pulmonary vascular complications in susceptible patient populations.
Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
Authors: Singh Archita , Roy Shradha , Singh Sharmila , Das Shabari Sarkar , Gautam Vibhav , Yadav Sandeep , Kumar Ashutosh , Singh Alka , Samantha Sukanya , Sarkar Ananda K. 
Published: June 2017

AbstractBoth phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.
Insulin-like growth factor I and its binding protein-3 are regulators of lactation and maternal responsiveness
Authors: Lékó András H. , Cservenák Melinda , Szabó Éva Rebeka , Hanics János , Alpár Alán , Dobolyi Árpád 
Published: June 2017

AbstractAdaptation to motherhood includes maternal behaviour and lactation during the postpartum period. The major organizing centres of maternal behaviour and lactation are located in the hypothalamic medial preoptic area (MPOA) and the arcuate nucleus, respectively. Insulin-like growth factor I (IGF-I) is an effector of the growth hormone axis; however, its function in the brain is largely unexplored. We identified increased maternal IGF binding protein-3 (IGFBP-3) expression in preoptic rat microarray data and confirmed it by RT-PCR. In situ hybridization histochemistry showed markedly elevated IGFBP-3 expression in the MPOA and the arcuate nucleus in rat dams. Prolonged intracerebroventricular injection of IGF-I or antagonism of brain IGFBP-3 with an inhibitor (NBI-31772) using osmotic minipumps increased pup retrieval time, suggesting reduced maternal motivation. Suckling-induced prolactin release and pup weight gain were also suppressed by IGF-I, suggesting reduced lactation. In addition, IGF-I-induced tyrosine hydroxylase expression and its specific phosphorylation in tuberoinfundibular dopaminergic neurons suppress prolactin secretion. Thus, IGF-I may inhibit both behavioural and lactational alterations in mothers. Neurons in the MPOA and arcuate nuclei express IGFBP-3 during the postpartum period to neutralize IGF-I effects. IGFBP-3 can prevent the blockade of maternal behaviour and lactation exerted by IGF-I, suggesting a novel modulatory mechanism underlying the behavioural and hormonal effects during central maternal adaptations.
Canine Leishmaniasis Progression is Associated with Vitamin D Deficiency
Authors: Rodriguez-Cortes A. , Martori C. , Martinez-Florez A. , Clop A. , Amills M. , Kubejko J. , Llull J. , Nadal J. M. , Alberola J. 
Published: June 2017

AbstractThe relationship between vitamin D deficiency and the risk of suffering from a plethora of health disorders, ranging from autoimmune processes to infectious diseases has been widely described. Nonetheless, the potential role of vitamin D in visceral leishmaniasis remains uncharacterized. In the Mediterranean basin, where the dog is leishmania’s main peri-domestic reservoir, control measures against the canine disease have shown beneficial effects on the incidence of human leishmaniasis. In this study, we measured the vitamin D levels in serum samples from a cohort of 68 healthy and disease dogs from a highly endemic area and we have also studied the relationship of these levels with parasitological and immunological parameters. The sick dogs presented significantly lower (P < 0.001) vitamin D levels (19.6 ng/mL) than their non-infected (31.8 ng/mL) and the asymptomatic counterparts (29.6 ng/mL). In addition, vitamin D deficiency correlated with several parameters linked to leishmaniasis progression. However, there was no correlation between vitamin D levels and the Leishmania-specific cellular immune response. Moreover, both the leishmanin skin test and the IFN-γ levels displayed negative correlations with serological, parasitological and clinical signs. Further studies to determine the functional role of vitamin D on the progression and control of canine leishmaniasis are needed.
Integrated analysis and transcript abundance modelling of H3K4me3 and H3K27me3 in developing secondary xylem
Authors: Hussey Steven G. , Loots Mattheus T. , van der Merwe Karen , Mizrachi Eshchar , Myburg Alexander A. 
Published: June 2017

AbstractDespite the considerable contribution of xylem development (xylogenesis) to plant biomass accumulation, its epigenetic regulation is poorly understood. Furthermore, the relative contributions of histone modifications to transcriptional regulation is not well studied in plants. We investigated the biological relevance of H3K4me3 and H3K27me3 in secondary xylem development using ChIP-seq and their association with transcript levels among other histone modifications in woody and herbaceous models. In developing secondary xylem of the woody model Eucalyptus grandis, H3K4me3 and H3K27me3 genomic spans were distinctly associated with xylogenesis-related processes, with (late) lignification pathways enriched for putative bivalent domains, but not early secondary cell wall polysaccharide deposition. H3K27me3-occupied genes, of which 753 (~31%) are novel targets, were enriched for transcriptional regulation and flower development and had significant preferential expression in roots. Linear regression models of the ChIP-seq profiles predicted ~50% of transcript abundance measured with strand-specific RNA-seq, confirmed in a parallel analysis in Arabidopsis where integration of seven additional histone modifications each contributed smaller proportions of unique information to the predictive models. This study uncovers the biological importance of histone modification antagonism and genomic span in xylogenesis and quantifies for the first time the relative correlations of histone modifications with transcript abundance in plants.
Evidence for Auditory-Motor Impairment in Individuals With Hyperfunctional Voice Disorders
Authors: Stepp Cara E. , Lester-Smith Rosemary A. , Abur Defne , Daliri Ayoub , Pieter Noordzij J. , Lupiani Ashling A. 
Published: June 2017

Purpose
The vocal auditory-motor control of individuals with hyperfunctional voice disorders was examined using a sensorimotor adaptation paradigm.

Method

Nine individuals with hyperfunctional voice disorders and 9 individuals with typical voices produced sustained vowels over 160 trials in 2 separate conditions: (a) while experiencing gradual upward perturbations in the fundamental frequency (
f
o
) of their auditory feedback (shift-up) and (b) under no auditory perturbation (control). The shift-up condition consisted of 4 ordered (fixed) phases: baseline (no perturbation), ramp (gradual increases in heard
f
o
), hold (a consistently higher heard
f
o
), and after-effect (no perturbation). Adaptive responses were defined as the difference in produced
f
o
during control and shift-up conditions.

Results

Adaptive responses were significantly different between groups. Individuals with typical voices generally showed compensatory adaptive responses, with decreased
f
o
during the ramp and hold phases. Conversely, many individuals with hyperfunctional voice disorders instead displayed the opposite effect by following the direction of the perturbation. When
f
o
was experimentally increased, speakers further increased their
f
o
.

Conclusion
Results indicate that some individuals diagnosed with hyperfunctional voice disorders have disrupted auditory-motor control, suggesting atypical neurological function. These findings may eventually allow for the development of new interventions for hyperfunctional voice disorders.
Development of an impedance spectroscopy device for on‐line cell growth monitoring
Authors: Montero‐Rodríguez J.J. , Fernández‐Castro A.J. , Schroeder D. , Krautschneider W. 
Published: June 2017

The impedance and dielectric properties of biological samples are often used to estimate the number of cells growing in a culture, and monitor the cell growth in real time. For these type of studies, high‐cost and complex commercial equipment is often required. The design and implementation of a standalone device for impedance spectroscopy of cell samples is presented, featuring low cost, portability and easy reconfigurability of frequency and voltage. The device can generate sinusoidal, square and triangular signals with frequencies up to 125 kHz, at voltages in the range from 0 V to 100 mV. Cell growth experiments were carried out with yeast cultures, and the results are compared with a commercial Agilent 4284A LCR meter. The error percentages for magnitude and phase measurements are <5%.
Compact low‐pass filter with ultra‐wide stopband using analysed triangular‐shaped resonator
Authors: Lotfi S. , Hayati M. 
Published: June 2017

A compact microstrip low‐pass filter (LPF) with ultra‐wide stopband using the analysed triangular‐shaped resonator is presented. To achieve LC equivalent circuit of the triangular‐shaped resonator, the structure of a rectangular‐shaped resonator is adopted. To investigate the behaviour of the proposed triangular‐shaped resonator, transfer function of the main triangular resonator is calculated. The designed LPF has −3 dB cut‐off frequency of 1.52 GHz with the stopband bandwidth around 12fc and total circuit size of 0.062λg × 0.104λg. The proposed LPF has the figure of merit of 46,718, which shows its good performance. The proposed filter is designed, simulated and fabricated. It is observed that the measurement results are well matched with simulation results.
Neonatal Immune Responses during Group B Streptococcal Infections
Authors: Upadhyay Kirtikumar , Talati Ajay 
Published: June 2017

AbstractGroup B streptococcus (GBS) still remains an important cause of neonatal sepsis in spite of various preventive strategies. The immune response of a neonate varies from an adult human immune system and makes a newborn more vulnerable to illness not typically manifested by adults. Microbial virulence, bacterial load, and immaturity of immune response system may explain the variation in severity of illness in term and preterm neonates. In this review, the mechanisms of GBS invasion and infection in a neonate are described. We also try to identify the host immune response to various bacterial components of GBS and possible future strategies to mitigate this immune response to improve neonatal outcomes after GBS sepsis.
Pulley Reconstruction for Symptomatic Instability of the Tendons of the First Extensor Compartment Following de Quervain's Release
Authors: Renson Daan , Mermuys Koen , Vanmierlo Bert , Bonte Francis , Van Hoonacker Petrus , Kerckhove Diederick , Berghs Bart , Goubau Jean 
Published: June 2017

Background Surgical management of de Quervain's tenosynovitis is based on decompression of the first extensor compartment. A simple release of the first compartment can cause instability of the extensor pollicis brevis (EPB) and abductor pollicis longus (APL) tendons in zone seven of the extensors. The WHAT test (wrist hyperflexion and abduction of the thumb) is very effective in diagnosing this instability.
Patients and Methods In this retrospective monocentric study, we analyzed a case series of 10 patients all of whom underwent a reconstruction of the first extensor compartment using a retinacular graft because of symptomatic instability after decompression surgery. The reconstruction was a modified technique of the sixth compartment. Functional outcome and characteristics of the newly reconstructed pulley were examined by physical examination with the aid of ultrasound and internationally validated questionnaires.
Results Four patients had a good-to-excellent functional outcome, all of those had a maximum of two surgical procedures performed on the first extensor compartment. Six patients presented poor functional outcome. In four of them, more than two surgical procedures were performed. Minor residual instability was noted in six cases, found in both the groups.
Conclusion The reconstruction procedures on the first compartment seemed to be satisfactory in treating instability of the EPB and APL tendons after primary surgical release for de Quervain's disease.
Level of Evidence Level IV, observational study without controls.
Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth: An Experimental Animal Study
Authors: Jeong Hyun , Kim Hee-youn , Kang Dahyun , Hong Soon-Cheol , Cho Geum , Oh Min-Jeong , Kim Hai-Joong , Ahn Ki 
Published: June 2017

Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice.
Study Design Sexually mature female C57BL/6 mice (n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed.
Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups (p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D (p = 0.037).
Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.
Demenz und Nährstoffe
Authors: Schmiedel Volker 
Published: June 2017

ZusammenfassungDemenz stellt ein komplexes Geschehen dar und es macht daher nur wenig Sinn, lediglich einen Faktor zu verändern. Eine ausreichende
Versorgung mit bestimmten Nährstoffen scheint Studien zufolge ein wichtiger Faktor zu sein, um die Hirnfunktion bis ins hohe Alter
zu erhalten. Dies hat sich für B-Vitamine, Vitamin D und Omega-3-Fettsäuren gezeigt. Probanden mit einer guten Versorgung wiesen
ein bis zu 50 % niedrigeres Risiko auf, an Demenz zu erkranken.Der Beitrag gibt eine Übersicht zu wichtigen Studien. Kasuistiken aus der Praxis des Autors bestätigen die Ergebnisse an konkreten
Patienten.
Patterns of Gall Bladder Wall Thickening in Dengue Fever: A Mirror of the Severity of Disease
Authors: Parmar Jitendra , Mohan Chander , Vora Maulik 
Published: June 2017

Abstract
Background Dengue fever is a major public health problem with an increased incidence in recent years. Gall bladder wall thickening has been reported as one of the most common findings in dengue fever. There is a paucity of literature regarding the various patterns of gall bladder wall thickening in dengue fever and their significance in predicting the severity of disease.
Methodology and Significant Findings Out of 93 seropositive patients included in the study, 54 patients with dengue fever had gall bladder wall thickening. 4 patterns of gall bladder wall thickening are demonstrated in this study. A uniform echogenic pattern in 20 patients, striated or tram track pattern in 11 patients, an asymmetric pattern in 2 patients and a honeycombing pattern in 21 patients. The range of patterns of wall thickening included normal wall thickening or uniform echogenic wall thickening in DF without warning signs, a striated or tram track pattern, and a honeycomb pattern in severe DF. Serial ultrasound done on consecutive alternate days revealed a change in the pattern of gall bladder wall thickening according to the severity of disease.
Conclusion The present study revealed 4 distinct patterns of gall bladder wall thickening. The uniform echogenic pattern was found to be more prevalent in dengue fever without warning signs, while the honeycomb pattern was found to be more prevalent in severe dengue fever. A change in the pattern of gall bladder wall thickening on subsequent serial ultrasound can predict the severity of the disease.
Aszites-Untersuchungen
Authors: Sengebusch Jürgen , Herzog Michael 
Published: June 2017

SummaryDie Untersuchung dient dazu, festzustellen, ob Fettgewebe oder ein Aszites Ursache für einen vergrößerten Bauchumfang ist. Eine Inspektion alleine erlaubt keine sichere Diagnose.
Prospective randomized comparison of a 22G core needle using standard versus capillary suction for EUS-guided sampling of solid pancreatic masses
Authors: Weston Brian , Ross William , Bhutani Manoop , Lee Jeffrey , Pande Mala , Sholl Andrew , Krishnamurthy Savitri 
Published: June 2017

Abstract
Background and study aims The optimal technique for sampling pancreatic lesions with a 22 G Procore needle (pc) is unknown. The aims of this study were to evaluate the 22 Gpc using standard suction technique (SST) and capillary suction technique (CST) and compare diagnostic adequacy of 22 Gpc with the standard 25 G needle.
Patients and methods Sixty consecutive patients referred for EUS-FNA of a solid pancreatic mass were prospectively evaluated. All patients underwent 2 passes with a standard 25 G needle for cytologic analysis. The first group of 30 patients underwent a single pass with the 22 Gpc needle using SST for cytology and histology. The second group underwent a single pass with the 22 Gpc needle using CST. The sequence of passes was randomized. The diagnostic adequacy of each pass was graded by 2 cytopathologists blinded to technique and needle type for comparison.
Results For a cytologic diagnosis with 22 Gpc, an adequate sample was obtained in 82.8 % SST vs. 80.0 % CST (P = 0.79). For a histologic diagnosis with 22 Gpc, an adequate sample was obtained in 70.4 % SST vs. 69.0 % CST (P = 0.91). A single pass with 22 Gpc provided comparable results to a single pass with the 25 G needle for a cytologic diagnosis; both were superior to a single 22 Gpc pass for a histologic diagnosis. Two passes with the 25 G needle provided a diagnostic specimen in 95.0 % vs 81.4 % with one pass using 22 Gpc (P = 0.01).
Conclusions No significant difference in diagnostic adequacy was observed between techniques for the 22 Gpc. Two passes with a 25 G needle performed better than 1 pass with 22 Gpc. (NCT01598194)Meeting presentations: Digestive Disease Week 2015
Lebenswelt Demenz und Ernährung
Authors: Gündling Andrea 
Published: June 2017

ZusammenfassungMenschen mit Demenz leben in eigenen Wirklichkeitsbereichen. Sie haben ein Anrecht darauf, dass ihre Gefühle und Grundbedürfnisse
respektiert werden. Nach dem Konzept der „Validation“ von Naomi Feil bedeutet das, die Realität und Lebenswelt, in der sich der
Demenzkranke befindet, wertzuschätzen. Gleichzeitig ergibt sich daraus aber auch eine ethisch-moralische Verpflichtung gegenüber
den Betroffenen, beispielsweise, wenn diese nicht mehr in ausreichendem Maße für ihre Ernährung sorgen können. Strategien wie die
Ernährungsbiografie oder gemeinsame Mahlzeiten in angenehmer Atmosphäre, helfen dabei, eine adäquate Nährstoffzufuhr sicherstellen
und Menschen mit Demenz vor Mangelernährung zu schützen. Zudem sollten ihren Vorlieben und veränderte Ess- und Trinkgewohnheiten
(z. B. nächtliches Hungergefühl) berücksichtigt werden.
Improving patient information – are the new media already requested? – A questionnaire study at a gastroenterology outpatient clinic
Authors: Walter Benjamin , Schmid Roland , von Delius Stefan 
Published: June 2017

Abstract
Background Information provided for patients is an essential factor for communication between patients and health-care professionals. To analyze the most used sources of medical information and requested media for patient information, a questionnaire study was initiated.
Methods A single-center questionnaire study at an outpatient clinic at a tertiary care hospital. Two hundred participating patients, average age 54.4 years (1:1.5 m:f). Results were displayed in total and as count per participant (i. e., how often is a medium mentioned per participant).
Results As a source for general information, printed media are mentioned 112 times (0.56 counts per participant), the Internet 125 (0.62), and television 124 (0.62). As a source for medical information, printed media were mentioned 84 times (0.42) and the Internet 133 (0.67). As the most requested source for additional patient information, printed media were mentioned 105 times (0.53) and medical apps 63 (0.32).
Conclusion A majority of our patients regularly use the Internet for medical information. Paper-print media are still highly requested by patients. New media are more often requested in younger patients but still reach the ages for screening programs and therefore offer big opportunities towards patient-doctor communication. By a good mixture of media provided a higher patient satisfaction and adherence could be ensured by the health-care professionals.
Die Haut mag’s bitter
Authors: Schempp Christoph M 
Published: June 2017

SummaryBitterstoffrezeptoren gibt es nicht nur auf der Zunge, sondern vermutlich auch im gesamten Magen-Darm-Trakt und in allen extraintestinalen Organen. Bitterstoffe aus dem Gelben Enzian und der Weidenrinde binden an Bitterstoffrezeptoren der Haut. Sie beleben den Hautstoffwechsel und stärken die Hautbarriere, zum Beispiel bei Neurodermitis. In der Anthroposophischen Medizin wendet man Bitterstoffe innerlich zur Behandlung von sogenannten Leberdermatosen wie Akne oder Rosazea an.