bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eine durch Lipid‐Wasser‐Trennung verstärkte FRET‐Sonde zur Detektion der Sauren Sphingomyelinase in lebenden Zellen
Authors: Pinkert Thomas , Furkert David , Korte Thomas , Herrmann Andreas , Arenz Christoph 
Published: February 2017

AbstractEine Echtzeit‐Beobachtung der Aktivität der Sauren Sphingomyelinase (ASM) ist notwendig zur Aufklärung ihrer Rolle in Lipid‐Signaltransduktionsprozessen. Mithilfe von Phosphorodichloridat‐Chemie gelang die Synthese von fluoreszierenden Phosphosphingolipiden mit der Fähigkeit zum FRET. Diese Sphingomyelin‐Analoga sind Substrate für die rekombinante humane ASM und ermöglichen die Verfolgung der ASM‐Aktivität mittels Fluoreszenzspektroskopie. Inkubation mit Zell‐Lysaten von Wildtyp‐ und Knockout‐Mäusen zeigte eine ausschließliche Spaltung durch ASM. Darüber hinaus nutzten wir systematisch die Umgebungsempfindlichkeit der Fluorophore, um signifikante Zuwächse in der Responsivität zu erzielen. Die dabei angewendeten Konzepte könnten auch für andere zukünftige Lipase‐Sonden zum Einsatz kommen. Eine mikroskopische Abbildung der ASM‐Aktivität in lebenden Zellen wurde mittels Zweiphotonenanregung realisiert.
Acceptorless Dehydrogenation of N‐Heterocycles by Merging Visible‐Light Photoredox Catalysis and Cobalt Catalysis
Authors: He Ke‐Han , Tan Fang‐Fang , Zhou Chao‐Zheng , Zhou Gui‐Jiang , Yang Xiao‐Long , Li Yang 
Published: February 2017

AbstractHerein, the first acceptorless dehydrogenation of tetrahydroquinolines (THQs), indolines, and other related N‐heterocycles, by merging visible‐light photoredox catalysis and cobalt catalysis at ambient temperature, is described. The potential applications to organic transformations and hydrogen‐storage materials are demonstrated. Primary mechanistic investigations indicate that the catalytic cycle occurs predominantly by an oxidative quenching pathway.
Multiple value dimensions spill‐over—An experimental approach in a consumption system comprising a product and a service
Authors: Arne Floh , Koller Monika , Zauner Alexander , Teller Christoph 
Published: February 2017

AbstractThrough the customer's eyes, wireless telecommunications are a typical example of a so‐called consumption system, comprising a product and a service subsystem. People consume an entity in which multiple value perceptions from both subsystems (wireless service and cell phone) are gained and affect attitudes, intentions and future behaviour within and across the subsystems. Value perceptions are gained along the dimensions of functionality, economic aspects, emotions and social facets, regarding both service and product. Some of those value perceptions spill over, from product to service and vice versa, while others do not. Those that spill over affect value perceptions and loyalty intentions in the other subsystem. These results provide the basis for deriving practical implications for the marketing management of firms operating in such a consumption system. Given the presence of spill‐over effects, both parties involved are advised to revise their marketing activities accordingly. Copyright © 2017 John Wiley & Sons, Ltd.
Synchronous radial 1H and 23Na dual‐nuclear MRI on a clinical MRI system, equipped with a broadband transmit channel
Authors: Kaggie Joshua D. , Sapkota Nabraj , Thapa Bijaya , Jeong Kyle , Shi Xianfeng , Morrell Glen , Bangerter Neal K. , Jeong Eun‐Kee 
Published: February 2017

AbstractThe purpose of this work was to synchronously acquire proton (1H) and sodium (23Na) image data on a 3T clinical MRI system within the same sequence, without internal modification of the clinical hardware, and to demonstrate synchronous acquisition with 1H/23Na‐GRE imaging with Cartesian and radial k‐space sampling. Synchronous dual‐nuclear imaging was implemented by: mixing down the 1H signal so that both the 23Na and 1H signal were acquired at 23Na frequency by the conventional MRI system; interleaving 1H/23Na transmit pulses in both Cartesian and radial sequences; and using phase stabilization on the 1H signal to remove mixing effects. The synchronous 1H/23Na setup obtained images in half the time necessary to sequentially acquire the same 1H and 23Na images with the given setup and parameters. Dual‐nuclear hardware and sequence modifications were used to acquire 23Na images within the same sequence as 1H images, without increases to the 1H acquisition time. This work demonstrates a viable technique to acquire 23Na image data without increasing 1H acquisition time using minor additional custom hardware, without requiring modification of a commercial scanner with multinuclear capability.
Theory of mind, affective empathy, and persuasive strategies in school‐aged children
Authors: Lonigro Antonia , Baiocco Roberto , Baumgartner Emma , Laghi Fiorenzo 
Published: February 2017

AbstractUnderstanding that other people hold different mental states and that they may be changed is the core of persuasion. Thus, theory of mind (ToM) abilities are fundamental to generate persuasive arguments. To date, only the relation between false belief and false belief‐emotion understanding and persuasion has been investigated, ignoring other advanced aspects of ToM. Moreover, the role of affective empathy in the engagement of persuasion strategies remains unknown. The aim of this study was to overcome such limitations. Elementary children (N = 142) were enrolled. An advanced ToM test, measures of affective empathy and verbal ability, and an assessment of persuasion, differentiated in self‐oriented and hetero‐oriented strategies, were administered to children. Results show that only ToM is a significant predictor of children's persuasive approach, after controlling for gender, age, and verbal ability. Affective empathy has a very marginal role in interaction with ToM only in the engagement of self‐oriented strategies. Limitations of the study and educational implications in school contexts are discussed.Highlights

The present study investigates the link between persuasive behaviour, affective empathy and theory of mind.
Through a cross-sectionial study, we find that mind reading abilities and affective empathy are linked to persuasive tactics as the result of a negation process.
The adoption of more advanced persuasive strategies may promote positive social relationships and help to resolve conflicting interactions.
Yield and Dry Matter Simulation Using the Saltmed Model for Five Quinoa (Chenopodium Quinoa) Accessions Under Deficit Irrigation in South Morocco
Authors: Kaoutar Filali , Abdelaziz Hirich , Ouafae Benlhabib , Redouane Choukr‐Allah , Ragab Ragab 
Published: February 2017

AbstractThe frequent drought in the Mediterranean region requires better water and crop management. Selection of suitable drought‐tolerant crops and the application of water‐saving strategies are essential.For this reason, a drought‐tolerant crop, quinoa, was selected to test its suitability to be grown under Morocco's conditions. In 2012, five accessions of quinoa (Chenopodium quinoa Willd.) were studied in a field in Agadir, Morocco. Four irrigation levels were applied; the control treatment received 100% of the crop water requirement and three other treatments received 75, 50 and 25% of the crop water requirement.The SALTMED model was calibrated using yield and total dry matter data obtained from the control treatment for each accession. The accessions differed in plant height, growth stage duration, harvest date, harvest index, leaf area index and photosynthesis efficiency. The SALTMED model simulated the total dry matter and grain yield for the five quinoa accessions under the different deficit irrigation regimes with reasonable precision, as confirmed by the values of coefficient of determination, R2 of 0.96 and 0.92, respectively.The results indicate that deficit irrigation at 75% would only reduce the yield of accession L142 by 5%. Therefore, L142 was recommended to the growers in Morocco. Copyright © 2017 John Wiley & Sons, Ltd.
Integration of CuAAC Polymerization and Controlled Radical Polymerization into Electron Transfer Mediated “Click‐Radical” Concurrent Polymerization
Authors: Xue Wentao , Wang Jie , Wen Ming , Chen Gaojian , Zhang Weidong 
Published: February 2017

The successful chain‐growth copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) polymerization employing Cu(0)/pentamethyldiethylenetriamine (PMDETA) and alkyl halide as catalyst is first investigated by a combination of nuclear magnetic resonance, gel‐permeation chromatography, and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. In addition, the electron transfer mediated “click‐radical” concurrent polymerization utilizing Cu(0)/PMDETA as catalyst is successfully employed to generate well‐defined copolymers, where controlled CuAAC polymerization of clickable ester monomer is progressed in the main chain acting as the polymer backbone, the controlled radical polymerization (CRP) of acrylic monomer is carried out in the side chain. Furthermore, it is found that there is strong collaborative effect and compatibility between CRP and CuAAC polymerization to improve the controllability.
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Growth of carbon and nitrogen containing precipitates in crystalline solar silicon and their influence on solar cells
Authors: Richter Susanne , Bauer Jan , Breitenstein Otwin 
Published: February 2017

magnified imageThe growth mechanisms, structural, mechanical, and electrical properties of silicon carbide and silicon nitride precipitates in solar silicon are reviewed and some new aspects about the distribution of trace elements in these precipitates are reported in this review. SiC and Si3N4 precipitates may have detrimental impact on the quality of solar silicon material. Therefore intensive research has been done at these precipitates in the past, which is summarized first. The properties of the different types of precipitates reported in literature are then described in detail. Especially SiC precipitates may have detrimental impact on the electrical behavior of solar cells by causing severe ohmic shunting. Furthermore SiC precipitates are very hard and may harm the wafering process. An outlook of the impact of such precipitates on new cell concepts and new solar silicon materials, such as n‐type, is given.
Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling
Authors: Tredwell Gregory D , Aw Rochelle , Edwards-Jones Bryn , Leak David J , Bundy Jacob G 
Published: February 2017

Abstract
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and supernatants) which correlated well with UPR-relevant gene transcripts, and so could be potential biomarkers for future high-throughput screening of large numbers of P. pastoris clones.
Metabolic syndrome or glucose challenge in first episode of psychosis?
Authors: Garcia-Rizo C. , Fernandez-Egea E. , Oliveira C. , Meseguer A. , Cabrera B. , Mezquida G. , Bioque M. , Penades R. , Parellada E. , Bernardo M. , Kirkpatrick B. 
Published: February 2017

AbstractPatients with schizophrenia exhibit a reduced life expectancy. Although unhealthy lifestyle or suicide risk plays a role, the main causes are diverse medical conditions such as cardiovascular diseases, type 2 diabetes mellitus and metabolic syndrome. Albeit pharmacological secondary side effects might also trigger previous conditions, studies in naïve patients reflect diverse anomalies at the onset. Patients with a first episode of psychosis, display a wide scope of metabolic abnormalities, ranging from normality till pathological values depending on the parameters studied. We attempted to evaluate the metabolic syndrome and glycemic homeostasis in a subset of antipsychotic-naïve patients with a first episode of non-affective psychosis. Patients (n = 84) showed a similar prevalence of metabolic syndrome compared with a matched control sample (n = 98) (6% vs 4%, P = 0.562), while glucose homeostasis values differed significantly (14% vs. 5%, P = 0.034). Our results suggest that metabolic syndrome is not a useful clinical condition to be evaluated in patients before pharmacological treatment. Abnormal glycemic homeostasis at the onset of the disease requires specific diagnostic tools and preventive measures in order to avoid future cardiovascular events. New strategies must be implemented in order to evaluate the cardiovascular risk and subsequent morbidity in patients at the onset of the disease.
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats with Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis
Authors: Cui Kai , Ruan Yajun , Wang Tao , Rao Ke , Chen Zhong , Wang Shaogang , Liu Jihong 
Published: February 2017

Abstract

Introduction
Erectile dysfunction (ED) in patients with diabetes mellitus (DM) seriously affects their quality of life. However, these patients show a poor effect rate for oral phosphodiesterase type 5 inhibitors. Thus, new treatment methods are urgently needed. Fingolimod hydrochloride (FTY720) was approved in 2010 for the treatment of patients with the relapsing-remitting form of multiple sclerosis.

Aim
To investigate whether FTY720 supplementation could ameliorate ED induced by DM (DMED).

Methods
Forty male Sprague-Dawley rats (8 weeks old) were used for the experiment. Thirty-two had type 1 DM induced by streptozotocin and the other eight rats constituted the control group. Eight weeks later, the erectile function of rats was assessed with an apomorphine test. Only some rats with DMED were treated with FTY720 orally every day for 4 weeks; the other rats remained in the same condition for 4 weeks.

Main Outcome Measure
Metabolic parameters; erectile function; sphingosine-1-phosphate receptor 3 (S1P3), protein kinase B (Akt), nitric oxide (NO), and cyclic guanosine monophosphate (cGMP) signaling pathway; corporal fibrosis; apoptosis level; and Smad and non-Smad signaling pathways.

Results
There were no significant differences in the initial body weights and fasting glucose concentrations among the three groups. Erectile function in the DMED group was significantly impaired compared with the control group and was partly, but significantly, improved in the DMED + FTY720 group. The DMED group showed inhibited activity of the S1P3-Akt-NO-cGMP signaling pathway, and the inhibition was partly reversed in the DMED + FTY720 group. The DMED group showed serious corporal fibrosis, higher apoptosis level, higher ratio of Bax to Bcl-2, and higher expressions of the Smad pathway (transforming growth factor-β1, Smad, and connective tissue growth factor) and the non-Smad pathway (transforming growth factor-β1, rho-associated protein kinase, LIM domain kinase 2, and cofilin). However, FTY720 supplementation partly increased the ratio of smooth muscle to collagen, decreased the ratio of Bax to Bcl-2, and inhibited activity of the Smad and non-Smad pathways.

Conclusion
FTY720 supplementation inhibited endothelial dysfunction and corporal fibrosis, ultimately leading to partial improvement of DMED in rats. This finding provides evidence for a potential treatment method for DMED.
Effect of intrathecal glucocorticoids on the central glucocorticoid receptor in a rat nerve ligation model
Authors: Rijsdijk Mienke , Agalave Nilesh M. , van Wijck Albert J.M. , Kalkman Cornelis J. , Ramachandran Roshni , Baharpoor Azar , Svensson Camilla I. , Yaksh Tony L. 
Published: February 2017

Abstract

Background and aims
Despite widespread use, the efficacy of neuraxial glucocorticoids for neuropathic painis subject to debate. Since most glucocorticoid actions are mediated through its receptor, we explored the effects of intrathecal methylprednisolone acetate (MPA) on total glucocorticoid receptor (tGR) levels and activation of the glucocorticoid receptor (phosphorylated state = pGR) within the spinal dorsal horn (SDH) and dorsal root ganglion (DRG) in a spinal nerve ligation (SNL) model in rats.

Methods
Rats received unilateral ligation of the L5/L6 spinal nerves and were treated with two intrathecal doses of either 400 μg MPA or 0.9% saline with a 72-h interval. Plantar tactile thresholds were measured over time. Seven days after drug treatment, DRG and SDH were harvested to assess tGR and pGR levels using immunohistochemistry and qPCR.

Results
Allodynia, defined by lowered tactile withdrawal thresholds after SNL, was unaltered by intrathecal MPA. In saline controls, mRNA levels of tGR did not change after SNL in the DRGs or SDH. tGR and pGR protein levels in the SDH however, significantly increased on the ipsilateral side of SNL compared to the contralateral side and to naïve tissue. When treating rats with MPA, tGR mRNA levels were significantly reduced in the SDH compared to saline controls. tGR and pGR protein levels, however were not significantly lower compared to saline controls.

Conclusions
In intrathecal MPA treated rats, tGR mRNA levels decreased after SNL. However this did not result in lower tGR and pGR protein levels compared to saline controls, and did not decrease ligation-induced mechanical hypersensitivity.

Implications
Intrathecal MPA treatment after SNL did not result in lower tGR and pGR levels within the SDH and DRG compared to saline controls. In present study we did not differentiate between the various isoforms of the GR which might clarify this finding.
THE ACCESS PARADOX: MEDIA ENVIRONMENT DIVERSITY AND COVERAGE OF ACTIVIST GROUPS IN JAPAN AND KOREA
Authors: Arrington Celeste L. 
Published: February 2017

AbstractTo what extent is a diverse news media environment good for activists who seek attention for their cause? Scholars agree that activist groups depend on the media to reach policymakers and bystanders. Yet prior scholars have overlooked how factors that contribute to media environment diversity—including journalistic norms, market structures, outlets’ partisanship, and audiences’ news consumption habits—can have contradictory implications for activist groups. Disaggregating questions of gaining publicity from questions of the message and reach of coverage, this article shows that while pluralistic media environments are more accessible to activists, more homogeneous media environments help groups that manage to break into the mainstream news reach wider audiences with more coherent narratives. These findings challenge common assumptions about the news media in Japan and Korea. A paired comparison of hepatitis C-related activism in both countries demonstrates how the forces democratizing access to the media are paradoxically reducing the persuasive potential of publicity.
Non-antibiotic approaches at drying-off for treating and preventing intramammary infections: a protocol for a systematic review and meta-analysis
Authors: Francoz David , Wellemans Vincent , Roy Jean-Philippe , Lacasse Pierre , Ordonez-Iturriaga Alfredo , Labelle François , Dufour Simon 
Published: February 2017

AbstractIntramammary infection (IMI) treatment and prevention at drying-off is one of the leading causes for using antimicrobials on dairy farms. The objective of the current paper is to describe the protocol used for conducting a systematic review of the literature on non-antibiotic strategies that can be used on dairy cows at dry off to treat and prevent IMI. Relevant literature will be identified using a combination of database search strategies and iterative screening of references. To be included in the review, articles will have to: (1) be published after 1969; (2) be written in English, French, or Spanish; (3) use a study design such as a controlled trial, an observational study, or an experimental study conducted in vivo; (4) be conducted on commercial dairy cows; (5) investigate a non-antibiotic intervention used at dry off; and finally, (6) report on a relevant mastitis outcome. Titles and abstracts, then full articles will be reviewed for inclusion. Specific data will be extracted and risk of bias will be assessed for all included articles. The planned systematic review will be the first to colligate, in a coherent whole, studies investigating non-antibiotic strategies for treating and preventing IMI at drying-off.
The inverse-trans-influence in tetravalent lanthanide and actinide bis(carbene) complexes
Authors: Gregson Matthew , Lu Erli , Mills David P. , Tuna Floriana , McInnes Eric J. L. , Hennig Christoph , Scheinost Andreas C. , McMaster Jonathan , Lewis William , Blake Alexander J. , Kerridge Andrew , Liddle Stephen T. 
Published: February 2017

AbstractAcross the periodic table the trans-influence operates, whereby tightly bonded ligands selectively lengthen mutually trans metal–ligand bonds. Conversely, in high oxidation state actinide complexes the inverse-trans-influence operates, where normally cis strongly donating ligands instead reside trans and actually reinforce each other. However, because the inverse-trans-influence is restricted to high-valent actinyls and a few uranium(V/VI) complexes, it has had limited scope in an area with few unifying rules. Here we report tetravalent cerium, uranium and thorium bis(carbene) complexes with trans C=M=C cores where experimental and theoretical data suggest the presence of an inverse-trans-influence. Studies of hypothetical praseodymium(IV) and terbium(IV) analogues suggest the inverse-trans-influence may extend to these ions but it also diminishes significantly as the 4f orbitals are populated. This work suggests that the inverse-trans-influence may occur beyond high oxidation state 5f metals and hence could encompass mid-range oxidation state actinides and lanthanides. Thus, the inverse-trans-influence might be a more general f-block principle.
Evolution of Shh endoderm enhancers during morphological transition from ventral lungs to dorsal gas bladder
Authors: Sagai Tomoko , Amano Takanori , Maeno Akiteru , Kimura Tetsuaki , Nakamoto Masatoshi , Takehana Yusuke , Naruse Kiyoshi , Okada Norihiro , Kiyonari Hiroshi , Shiroishi Toshihiko 
Published: February 2017

AbstractShh signalling plays a crucial role for endoderm development. A Shh endoderm enhancer, MACS1, is well conserved across terrestrial animals with lungs. Here, we first show that eliminating mouse MACS1 causes severe defects in laryngeal development, indicating that MACS1-directed Shh signalling is indispensable for respiratory organogenesis. Extensive phylogenetic analyses revealed that MACS1 emerged prior to the divergence of cartilaginous and bony fishes, and even euteleost fishes have a MACS1 orthologue. Meanwhile, ray-finned fishes evolved a novel conserved non-coding sequence in the neighbouring region. Transgenic assays showed that MACS1 drives reporter expression ventrally in laryngeal epithelium. This activity has been lost in the euteleost lineage, and instead, the conserved non-coding sequence of euteleosts acquired an enhancer activity to elicit dorsal epithelial expression in the posterior pharynx and oesophagus. These results implicate that evolution of these two enhancers is relevant to the morphological transition from ventral lungs to dorsal gas bladder.