bluuebunny/crossref_metadata_2025_split huggingface dataset:
INDICATION, IDENTIFICATION AND INACTIVATION SALMONELLA ABORTUS-OVIS
Authors: Mussayeva А.К. , Egorova N.N. , Yerishov M.O. , Tlepov A.A. , Kassenov M.M. 
Published: October 2024

The article presents the results of identification of an epizootic culture of Salmonella abortus-ovis (A) 58 isolated from parenchymatous organs of an aborted ovine fetus, inv. No. KZH 210397333, delivered from the village of Senokosnoye, Osakarovsky district, Karaganda region. To isolate a pure culture of salmonella from pathological material from the abort fetus of a ovine, the method of stepwise selection of colonies according to Drigalsky was used. The identification of the isolated culture was carried out on the basis of studying the cultural-morphological, biochemical, and antigenic properties of Salmonella abortus-ovis culture (A) 58. The culture was agglutinated with polyvalent and monoreceptor salmonella sera. According to the results of typing in AR, the culture isolated from the aborted ovine fetus was assigned to the genus Salmonella, the species abortus-ovis, serological group B. The article presents the results of experiments to optimization the process of salmonella cultivation. The passive matrix cultures of salmonella of the second generation in the exponential growth phase were used to cultivate the epizootic S. abortus-ovis (A) 58 strain. Periodic cultivation of salmonella ensured the physiologically active state of the culture, an increase in the maximum growth rate, a reduction in lag phase and a minimum generation time. The salmonella inactivation regime has been worked out, followed by seeding into liquid and dense nutrient media to determine the completeness of inactivation. It was found that effective inactivants are 10% solution of dimerethylenimine (DEI) and a 10% solution of formalin. The article presents the conditions of reactivation and cultivation of the S. abortus—ovis strain (A) 58.
Recent advances in the influenza virus vaccine landscape: a comprehensive overview of technologies and trials
Authors: Clark Tristan W. , Tregoning John S. , Lister Helen , Poletti Tiziano , Amin Femy , Nguyen-Van-Tam Jonathan S. 
Published: October 2024

SUMMARY
In the United Kingdom (UK) in 2022/23, influenza virus infections returned to the levels recorded before the COVID-19 pandemic, exerting a substantial burden on an already stretched National Health Service (NHS) through increased primary and emergency care visits and subsequent hospitalizations. Population groups ≤4 years and ≥65 years of age, and those with underlying health conditions, are at the greatest risk of influenza-related hospitalization. Recent advances in influenza virus vaccine technologies may help to mitigate this burden. This review aims to summarize advances in the influenza virus vaccine landscape by describing the different technologies that are currently in use in the UK and more widely. The review also describes vaccine technologies that are under development, including mRNA, and universal influenza virus vaccines which aim to provide broader or increased protection. This is an exciting and important era for influenza virus vaccinations, and advances are critical to protect against a disease that still exerts a substantial burden across all populations and disproportionately impacts the most vulnerable, despite it being over 80 years since the first influenza virus vaccines were deployed.
Toward better cures for
Mycobacterium abscessus
lung disease
Authors: Dartois Véronique , Dick Thomas 
Published: October 2024

SUMMARY

The opportunistic pathogen
Mycobacterium abscessus
(Mab) causes fatal lung infections that bear similarities—and notable differences—with tuberculosis (TB) pulmonary disease. In contrast to TB, no antibiotic is formally approved to treat Mab disease, there is no reliable cure, and the discovery and development pipeline is incredibly thin. Here, we discuss the factors behind the unsatisfactory cure rates of Mab disease, namely intrinsic resistance and persistence of the pathogen, and the use of underperforming, often parenteral and toxic, repurposed drugs. We propose preclinical strategies to build injectable-free sterilizing and safe regimens: (i) prioritize oral bactericidal antibiotic classes, with an initial focus on approved agents or advanced clinical candidates to provide immediate options for desperate patients, (ii) test drug combinations early, (iii) optimize novel leads specifically for
M. abscessus
, and (iv) consider pharmacokinetic-pharmacodynamic targets at the site of disease, the lung lesions in which drug tolerant bacterial populations reside. Knowledge and tool gaps in the preclinical drug discovery process are identified, including validated mouse models and computational platforms to enable
in vitro
mouse-human translation. We briefly discuss recent advances in clinical development, the need for readouts and biomarkers that correlate with cure, and clinical trial concepts adapted to the uniqueness of Mab patient populations for new regimen development. In an era when most pharmaceutical firms have withdrawn from antimicrobial drug discovery, the breakthroughs needed to fill the regimen development pipeline will likely come from partnerships between academia, biotech, pharma, non-profit organizations, and governments, with incentives that reward cooperation.
Supplemental Material: The development of a pull-apart basin in response to collision of the irregular South China continental margin with Indochina
Authors: Ma Si-Yuan , al. et 
Published: October 2024

<p>Table S1: LA-ICP-MS zircon U-Pb dating results for Lopingian Pengjiu Formation in the Shiwandashan basin. Figure S1: Photomicrographs of representative detrital zircon grains in distinct age populations. </p>
Canadian College of Medical Geneticists: clinical practice advisory document – responsibility to recontact for reinterpretation of clinical genetic testing
Authors: Goh Elaine Suk-Ying , Chad Lauren , Richer Julie , Bombard Yvonne , Mighton Chloe , Agatep Ron , Lacaria Melanie , Penny Blaine , Thomas Mary Ann , Zawati Ma'n H , MacFarlane Julie , Laberge Anne-Marie , Nelson Tanya N 
Published: October 2024

BackgroundAdvances in technology and knowledge have facilitated both an increase in the number of patient variants reported and variants reclassified. While there is currently no duty to recontact for reclassified genetic variants, there may be a responsibility. The purpose of this clinical practice advisory document is to provide healthcare practitioners guidance for recontact of previously identified and classified variants, suggest methods for recontact, and principles to consider, taking account patient safety, feasibility, ethical considerations, health service capacity and resource constraints. The target audience are practitioners who order genetic testing, follow patients who have undergone genetic testing and those analysing and reporting genetic testing.MethodsA multidisciplinary group of laboratory and ordering clinicians, patient representatives, ethics and legal researchers and a genetic counsellor from the Canadian Association of Genetic Counsellors reviewed the existing literature and guidelines on responsibility to recontact in a clinical context to make recommendations. Comments were collected from the Canadian College of Medical Geneticists (CCMG) Education, Ethics, and Public Policy, Clinical Practice and Laboratory Practice committees, and the membership at large.ResultsFollowing incorporation of feedback, and external review by the Canadian Association of Genetic Counsellors and patient groups, the document was approved by the CCMG Board of Directors. The CCMG is the Canadian organisation responsible for certifying laboratory and medical geneticists who provide medical genetics services, and for establishing professional and ethical standards for clinical genetics services in Canada.ConclusionThe document describes the ethical and practical factors and suggests a shared responsibility between patients, ordering clinician and laboratory practitioners.
Visualization, Dynamicity, and Collaborative Networking of Scientific Production on Visible Light and Skin Aging: A Scientometric Analysis
Authors: Espinoza-Carhuancho Fran , Quispe-Vicuña Carlos , Mauricio-Vilchez Cesar , Galarza-Valencia Diego , Medina Julia , Pacheco-Mendoza Josmel , Mayta-Tovalino Frank 
Published: October 2024

Purpose: The purpose of this is to examine the visualization, dynamicity, and collaborative networking of scientific production on visible light (VL) and skin aging through scientometric analysis.Materials and Methods: This research consisted of a cross‐sectional and descriptive design with a scientometric approach that examined the publication trends and collaborative patterns among authors and institutions from 2018 to 2023. A comprehensive search strategy was also employed by using specific keywords related to VL and skin aging. In this case, several indicators were employed, including scholarly output, view count, field‐weighted citation impact (FWCI), and citation count. The analyses were performed by using SciVal software and R Studio version 4.3.2.Results: A total of 180 sources were identified, with 280 documents generated, indicating an annual growth rate of 6.72%. The documents, averaging 3.25 years in age, received an average of 12.14 citations, revealing their impact. Additionally, collaborations were evident, with a ratio of 5.6 coauthors per paper and 25.71% consisting of international collaborations. In terms of institutions, there were notable disparities in scholarly activities and impact metrics, highlighting the diversity of the research landscape. Meanwhile, journals, such as Photodermatology, Photoimmunology and Photomedicine, revealed a substantial impact (FWCI 2.05). Overall, the impact of the journals showed a general upward trend, reflecting dynamicity and variability over time.Conclusion: An annual growth rate of 6.72% was found, with 180 sources and 280 papers on VL and skin aging. Moreover, international collaborations, the positive impact in leading journals, and the distribution patterns identified through scientometric laws underscored the vitality and complexity of the field. These results offer valuable insights into guiding future research in this multidisciplinary field.
The Effect of Exposure to Mobile Phones on Electrical Cardiac Measurements: A Multivariate Analysis and a Variable Selection Algorithm to Detect the Relationship With Mean Changes
Authors: Alharbi Nader , Alassiri Mohammed 
Published: October 2024

Background: The exponential growth in mobile phone usage has raised concerns about electromagnetic field (EMF) exposure and its health risks. Blood pressure and BMI, which impair heart function due to decreased adrenoreceptor responsiveness, parasympathetic tone withdrawal, and increased sympathetic activity, may further exacerbate these risks. However, the effects of radiofrequency electromagnetic (RF‐EM) exposure from mobile phones on electrocardiograms (ECGs) and heart rate variability (HRV) in individuals remain unclear.Purpose: Building upon our previous findings on HRV changes due to mobile phone proximity, this study is aimed at significantly enhancing the analytical approach used to assess the effects of mobile phones on cardiac parameters. This study exploits data from a previous study but with a different purpose. The aim of this study is twofold: (a) to examine whether exposure to mobile phones changes the five variables (P‐R, QRS, QT, ST, and HR) in a multivariate manner and (b) to examine whether the blood pressure and/or the body mass index (BMI), which acts as a proxy for obesity, have an effect on the change of these five variables. For both aspects of the study, four cycles are performed.Method: We conducted multivariate analysis on previously collected electrical cardiac measurement data from 20 healthy male subjects exposed to mobile phone EMF, with the mobile phones placed at four different body locations. The one‐sample Hotelling T2 test on the mean vector of differences was utilised instead of multiple paired t‐tests. This multivariate method comprehensively analyzes data features and accounts for variable correlations, unlike multiple univariate analyses. Given our small sample size, we employed the MMPC variable selection algorithm to identify predictor variables significantly related to mean changes.Results: Significant alterations in ECG intervals and heart rate were noted in the subjects before and after the first EMF exposure cycle, independent of their BMI. Notably, heart rate, P‐R, and QRS intervals fell postexposure while QT and ST intervals increased. These changes were influenced by variations in systolic blood pressure, with BMI showing no significant effect.Conclusion: The observed modifications in cardiac electrical measurements due to mobile phone EMF exposure are attributed to the effects of EMF itself, with no impact from BMI on the extent of these changes.
Career Orientations of Pre-Service Teachers: Exploring the Influence of Different Types of Universities
Authors: Valeeva Roza A. , Parfilova Gulfiia G. , Kremen Faina M. , Kremen Sergei A. 
Published: October 2024

Background. More than half of future teachers in our country receive professional training in classical universities, which determines a great variability of motivational-professional and planned career trajectories. At the same time, the type of university (federal or regional) significantly influences the conditions for forming a professional teacher. This study identifies career orientations of students attending classical universities of different types in order to determine trends in career preferences according to choice of university type.	Objective. This study aims to identify the peculiarities of career orientations of students studying at pedagogical bachelor degree programs of classical universities of different types (federal and regional), determining the interrelations of their indicators with socio-demographic and motivational-professional characteristics of the test subjects.	Design. The study was conducted in the form of a comparison of career orientations of the first-year students in teacher education programs (83 students of Kazan Federal University and 89 students of Smolensk State University). The empirical methods used included the adapted Schein's career anchors tool and a questionnaire revealing socio-demographic and motivational-professional variables to identify factors related to career orientations.	Results. The obtained results revealed common preferences for both universities in career orientations on the service, job stability and lifestyle integration scales, indicating stable trends in choosing the teaching profession. Students at both universities, who chose the teaching profession and plan to work in their specialty, demonstrate a readiness for professional development and overcoming difficulties. However, the university is an independent factor which determines differences according to the scales of entrepreneurship, autonomy and management. Students at a federal university are characterized by greater independence and initiative, but also more uncertainty in choosing a profession, in contrast to students at a regional university.	Conclusion. Considering student career orientations according to their socio-demographic and motivational-professional characteristics, allows us to obtain an objective and comprehensive picture of the professional choices of students from different types of universities, leading to more effective delivery of their professional development.
How Spatial Differences in Climate Affect Agricultural Land Values in New Zealand
Authors: Pourzand Farnaz 
Published: October 2024

Abstract
This paper implements the “spatial first differences” (SFD) method to a Ricardian approach to analyze spatial differences in climate and evaluate their impact on farmland values in New Zealand. We use property valuation data from 1993 to 2018 across different agricultural land uses. Ricardian analyses harness cross-sectional variation in climate and farmland values to estimate the effect of climate on agriculture. However, Ricardian studies are vulnerable to omitted variables varying slowly or not varying over time. The idea of the SFD approach is to apply spatial analogous of the “first-differences” estimator for longitudinal analyses to control for omitted variables that vary differently than climate variables among neighboring units. The results suggest that a warmer or drier climate is associated with higher farmland values in New Zealand. These findings may be mediated by productivity differences, costly physical improvements, and climate amenity values. Besides contributing novel insights by applying the Ricardian approach to New Zealand data and overcoming challenges of unobserved heterogeneity with the SFD method, we conclude that, for a better understanding of the welfare implications of climate change, it is crucial to differentiate between the effects of costly improvements and amenity values, apart from the pure climate productivity effect.
Attitudes toward implementation of electronic medical record and its associated factors among health professional workers in selected public hospitals in Addis Ababa, Ethiopia, 2023: A multi-center cross-sectional study
Authors: Mohammed Abdurehman Seid , Wudu Desalgn , Minda Zewdu , Diress Getachew Mekete 
Published: October 2024

Background study Electronic medical record (EMR) systems in healthcare delivery have the potential to transform healthcare in terms of saving costs, reducing medical errors, and improving data quality. This study aimed to assess the Attitudes toward implementing electronic medical records and associated factors among health professional workers in selected public hospitals in Addis Ababa. Method An institution-based cross-sectional study was conducted on 422 health professionals in selected hospitals in Addis Ababa, Ethiopia, in 2023. The study participants were selected using a simple random sampling technique. A binary and multivariable logistic regression model was used to identify associated factors for electronic medical record implementation. A p-value < .05 was considered statistically significant. Result The overall electronic medical record implementation perceived as useful by health workers is 298 (73.6%), with an allocation of enough budget [AOR = 3.196 (1.49–6.735)] has no networking or problem with internet access [AOR = 1.794(1.089–2.954)]. Electronic medical record increases workload [AOR = 2.350 (1.302–4.243)], which was significantly associated with electronic medical record implementation. Conclusion and Recommendation According to this study, the overall perception of health professionals toward electronic medical record implementation was high. However, it would be better to build and establish strong internet connectivity and stable power supply or internet access without networking problems, allocate enough budget, and work in collaboration with hospitals and health bureaus to strengthen and support the electronic medical record in their facilities.
Thermosensing ability of TRPC5: current knowledge and unsettled questions
Authors: Ptakova Alexandra , Vlachova Viktorie 
Published: October 2024

AbstractOur understanding of how the mammalian somatosensory system detects noxious cold is still limited. While the role of TRPM8 in signaling mild non-noxious coolness is reasonably understood, the molecular identity of channels transducing painful cold stimuli remains unresolved. TRPC5 was originally described to contribute to moderate cold responses of dorsal root ganglia neurons in vitro, but mice lacking TRPC5 exhibited no change in behavioral responses to cold temperature. The question of why a channel endowed with the ability to be activated by cooling contributes to the cold response only under certain conditions is currently being intensively studied. It seems increasingly likely that the physiological detection of cold temperatures involves multiple different channels and mechanisms that modulate the threshold and intensity of perception. In this review, we aim to outline how TRPC5 may contribute to these mechanisms and what molecular features are important for its role as a cold sensor.
Inhibition of malate dehydrogenase via nanoselenium coupling with nanocellulose composite in MCF-7 breast carcinoma cells
Authors: El-Sheekh Mostafa M. , Kenawy El-Refaie , Yousuf Wesam E. , Mohamed Tarek M. 
Published: October 2024

Abstract
Background
Cancer, a leading cause of mortality worldwide, continues to pose significant challenges in treatment and management. Conventional therapies often face limitations, including lack of selectivity, adverse effects, and the development of resistance mechanisms.

Methods
Therefore, this study aims to investigate nanocellulose, nanoselenium, and their nanocomposite which are previously synthesized and characterized. Molecular docking simulations were performed to assess binding affinity to malate dehydrogenase-1 (MDH-1), a key metabolic enzyme in cancer cells. Cytotoxicity was evaluated in A549 lung cancer cell line, the MCF-7 breast cancer cell line, and the WI-38 normal cell line. Mechanistic studies included assessment of MDH-1 activity and expression, intracellular reactive oxygen species (ROS) levels, and cell cycle analysis.

Results
Molecular docking simulations demonstrated a favorable binding affinity (136.98 kcal/mol) of cellulose and selenium as cofactor to the nicotinamide adenine dinucleotide (NAD) + hydrogen (H) (NADH) binding domain of human MDH-1. The nanocomposite exhibited a synergistic impact against cancer, causing a considerable decrease in the viability of MCF-7 cells compared to separate treatments with nanocellulose and nanoselenium. Moreover, it showed negligible toxicity towards normal cells. Biochemical studies demonstrated that nanocellulose, nanoselenium and the nanocomposite substantially reduced MDH-1 activity and messenger ribonucleic acid (mRNA) expression in MCF-7 cells. This was confirmed by flow cytometric analysis, which revealed that the nanocomposite could effectively reduce the intracellular ROS levels and induce potent cell cycle arrest in the G0/G1 phase, that inhibit MCF-7 cell proliferation.

Conclusions
In conclusion, our finding elucidated the promising therapeutic potential of nanocellulose, nanoselenium, and their nanocomposite as effective anticancer agents in breast cancer treatment, demanding further preclinical and clinical investigations to explain their mechanisms of action.