bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgender Health Care for Nurses: An Innovative Approach to Diversifying Nursing Curricula to Address Health Inequities
Authors: McDowell Alex , Bower Kelly M. 
Published: July 2016

Background:
Transgender people experience high rates of discrimination in health care settings, which is linked to decreases in physical and mental wellness. By increasing the number of nurses who are trained to deliver high-quality care to transgender patients, health inequities associated with provider discrimination can be mitigated. At present, baccalaureate nursing curricula do not adequately prepare nurses to care for transgender people, which is a shortcoming that has been attributed to limited teaching time and lack of guidance regarding new topics.

Method:
We developed transgender health content for students in a baccalaureate nursing program and used a student–faculty partnership model to integrate new content into the curriculum.

Results:
We incorporated new transgender health content into five required courses over three semesters.

Conclusion:

We mitigated common barriers to developing and integrating new, diversity-related topics into a baccalaureate nursing curriculum. Added transgender health content was well received by students and faculty. [
J Nurs Educ.
2016;55(8):476–479.]
FcγRIIB-Independent Mechanisms Controlling Membrane Localization of the Inhibitory Phosphatase SHIP in Human B Cells
Authors: Pauls Samantha D. , Ray Arnab , Hou Sen , Vaughan Andrew T. , Cragg Mark S. , Marshall Aaron J. 
Published: July 2016

Abstract
SHIP is an important regulator of immune cell signaling that functions to dephosphorylate the phosphoinositide phosphatidylinositol 3,4,5-trisphosphate at the plasma membrane and mediate protein–protein interactions. One established paradigm for SHIP activation involves its recruitment to the phospho-ITIM motif of the inhibitory receptor FcγRIIB. Although SHIP is essential for the inhibitory function of FcγRIIB, it also has critical modulating functions in signaling initiated from activating immunoreceptors such as B cell Ag receptor. In this study, we found that SHIP is indistinguishably recruited to the plasma membrane after BCR stimulation with or without FcγRIIB coligation in human cell lines and primary cells. Interestingly, fluorescence recovery after photobleaching analysis reveals differential mobility of SHIP–enhanced GFP depending on the mode of stimulation, suggesting that although BCR and FcγRIIB can both recruit SHIP, this occurs via distinct molecular complexes. Mutagenesis of a SHIP–enhanced GFP fusion protein reveals that the SHIP–Src homology 2 domain is essential in both cases whereas the C terminus is required for recruitment via BCR stimulation, but is less important with FcγRIIB coligation. Experiments with pharmacological inhibitors reveal that Syk activity is required for optimal stimulation-induced membrane localization of SHIP, whereas neither PI3K or Src kinase activity is essential. BCR-induced association of SHIP with binding partner Shc1 is dependent on Syk, as is tyrosine phosphorylation of both partners. Our results indicate that FcγRIIB is not uniquely able to promote membrane recruitment of SHIP, but rather modulates its function via formation of distinct signaling complexes. Membrane recruitment of SHIP via Syk-dependent mechanisms may be an important factor modulating immunoreceptor signaling.
Writer’s cramp in spinocerebellar ataxia Type 1
Authors: Khwaja Geeta Anjum , Srivastava Abhilekh , Ghuge Vijay Vishwanath , Chaudhry Neera 
Published: July 2016

ABSTRACTDystonia can be encountered in a small subset of patients with spinocerebellar ataxia (SCA), but task specific dystonia is extremely rare. We report a case of a 48-year-old male with confirmed SCA Type 1 (SCA1) with mild progressive cerebellar ataxia and a prominent and disabling Writer’s cramp. This case highlights the ever-expanding phenotypic heterogeneity of the SCA’s in general and SCA1 in particular.
‘The Law BV’: heeft het bewijsvoordeel in artikel 2:138 lid 2/248 lid 2 BW zijn langste tijd gehad?
Authors: de Groot C. 
Published: July 2016

‘The Law BV’: heeft het bewijsvoordeel in artikel 2:138 lid 2/248 lid 2 BW zijn langste tijd gehad?
Op 12 februari 2016 heeft de Hoge Raad een uitspraak gedaan in een bestuurdersaansprakelijkheidsprocedure op grond van artikel 2:248 lid 2 BW. Uit de uitspraak komt naar voren dat het bewijsvoordeel dat de curator aan artikel 2:248 lid 2 BW kan ontlenen veel van zijn betekenis heeft verloren.
Balanceren tussen een taakverdeling en collegialiteit binnen de raad
Authors: in ’t Veld L. 
Published: July 2016

Balanceren tussen een taakverdeling en collegialiteit binnen de raad. De verhouding tussen auditcommissieleden en overige commissarissen bezien in het licht van geagendeerde wet- en regelgeving
De auditcommissie heeft een vaste positie verworven binnen het Nederlandse vennootschapsrecht. Recente ontwikkelingen accentueren de onduidelijkheid die bestaat over de verhouding tussen auditcommissieleden en overige leden van de raad. De auteur meent dat het evenwicht dient te worden bewaakt tussen een ver(der)gaande taakverdeling en het beginsel van collegialiteit binnen de raad.
Adult Human Peripheral Blood Mononuclear Cells Are Capable of Producing Neurocyte or Photoreceptor-Like Cells That Survive in Mouse Eyes After Preinduction With Neonatal Retina
Authors: Xian Bikun , Zhang Yichi , Peng Yuting , Huang Jianfa , Li Weihua , Wang Wencong , Zhang Min , Li Kaijing , Zhang Hening , Zhao Minglei , Liu Xing , Huang Bing 
Published: July 2016

AbstractAdult human peripheral blood mononuclear cells (hPBMCs) exhibit pluripotency in vitro and so may be a valuable cell source for regenerative therapies. The efficacy of such therapies depends on the survival, differentiation, migration, and integration capacity of hPBMCs in specific tissues. In this study, we examined these capacities of transplanted hPBMCs in mouse retina as well functional improvement after transplant. We isolated hPBMCs and preinduced them for 4 days in media preconditioned with postnatal day 1 rat retina explants. Preinduction increased the proportions of hPBMCs expressing neural stem cell, neural progenitor cell, or photoreceptor markers as revealed by immunofluorescent staining, flow cytometry, and quantitative real-time polymerase chain reaction. Preinduced hPBMCs were transplanted into the subretinal space of retinal degenerative slow (RDS) and retinal degeneration 1 (RD1) mice. At 1, 3, and 6 months after transplantation, treated eyes of RDS mice were collected and cell phenotype was studied by immunofluorescent staining. Preinduced hPBMCs survived in the subretinal space; migrated away from the injection site and into multiple retinal layers; and expressed neural stem cell, neuronal, and photoreceptor markers. Finally, we assessed RD1 retinal function after subretinal transplantation and found significant improvement at 3 months after transplantation. The ease of harvesting, viability in vivo, capacity to express neuronal and photoreceptor proteins, and capacity for functional enhancement suggest that hPBMCs are potential candidates for cell replacement therapy to treat retinal degenerative diseases.SignificanceThis study provides support for the use of peripheral blood mononuclear cells (PBMCs) as a potential source of pluripotent stem cells for treating retinal degeneration. First, this study demonstrated that PBMCs can differentiate into retinal neuron-like cells in vitro and in vivo. Second, some transplanted cells expressed markers for neural progenitors, mature neurons, or photoreceptors at 1, 3, and 6 months after subretinal injection. Finally, this study showed that PBMC transplantation can improve the function of a degenerated retina.
Concise Review: In Vitro Formation of Bone-Like Nodules Sheds Light on the Application of Stem Cells for Bone Regeneration
Authors: Mechiche Alami Saad , Gangloff Sophie C. , Laurent-Maquin Dominique , Wang Yun , Kerdjoudj Halima 
Published: July 2016

Abstract
Harnessing the differentiation of stem cells into bone-forming cells represents an intriguing avenue for the creation of functional skeletal tissues. Therefore, a profound understanding of bone development and morphogenesis sheds light on the regenerative application of stem cells in orthopedics and dentistry. In this concise review, we summarize the studies deciphering the mechanisms that govern osteoblast differentiation in the context of in vitro formation of bone-like nodules, including morphologic and molecular events as well as cellular contributions to mineral nucleation, occurring during osteogenic differentiation of stem cells. This article also highlights the limitations of current translational applications of stem cells and opportunities to use the bone-like nodule model for bone regenerative therapies.

Significance
Harnessing the differentiation of stem cells into bone-forming cells represents an intriguing avenue for the creation of functional skeletal tissues. Therefore, a profound understanding of bone development and morphogenesis sheds light on the regenerative application of stem cells in orthopedics and dentistry. In this concise review, studies deciphering the mechanisms that govern osteoblast commitment and differentiation are summarized. This article highlights the limitations of current translational applications of stem cells and the opportunities to use the bone-like nodule model for bone regenerative therapies.
Flower diversity and bee reproduction in an arid ecosystem
Authors: Dorado Jimena , Vázquez Diego P. 
Published: July 2016

Background:Diverse flower communities are more stable in floral resource production along the flowering season, but the question about how the diversity and stability of resources affect pollinator reproduction remains open. High plant diversity could favor short foraging trips, which in turn would enhance bee fitness. In addition to plant diversity, greater temporal stability of floral resources in diverse communities could favor pollinator fitness because such communities are likely to occupy the phenological space more broadly, increasing floral availability for pollinators throughout the season. In addition, this potential effect of flower diversity on bee reproduction could be stronger for generalist pollinators because they can use a broader floral spectrum. Based on above arguments we predicted that pollinator reproduction would be positively correlated to flower diversity, and to temporal stability in flower production, and that this relationship would be stronger for the most generalized pollinator species.Materials and Methods:Using structural equation models, we evaluated the effect of these variables and other ecological factors on three estimates of bee reproduction (average number of brood cells per nest per site, total number of brood cells per site, and total number of nests per site), and whether such effects were modulated by bee generalization on floral resources.Results:Contrary to our expectations, flower diversity had no effect on bee reproduction, stability in flower production had a weakly negative effect on one of the bee reproductive variables, and the strength of the fitness-diversity relationship was unrelated to bee generalization. In contrast, elevation had a negative effect on bee reproduction, despite the narrow elevation range encompassed by our sites.Discussion:Flower diversity did not affect the reproduction of the solitary bees studied here. This result could stem from the context dependence of the diversity-stability relationship, given that elevation had a positive effect on flower diversity but a negative effect on bee reproduction. Although high temporal stability in flower production is expected to enhance pollinator reproduction, in our study it had a weakly negative—instead of positive—effect on the average number of brood cells per nest. Other environmental factors that vary with elevation could influence bee reproduction. Our study focused on a small group of closely-related bee species, which cautions against generalization of our findings to other groups of pollinators. More studies are clearly needed to assess the extent to which pollinator demography is influenced by the diversity of floral resources.
Atuação do enfermeiros da Estratégia Saúde da Família na prevenção do pé diabético Practice nurse family health strategy in the prevention of diabetic foot
Authors: Oliveira Patrícia Simplício de , Bezerra Eva Porto , Andrade Lidiane Lima de , Gomes Priscila Laís Ferreira , Soares Maria Júlia Guimarães Oliveira , Costa Marta Miriam Lopes 
Published: July 2016

Objetivos: Identificar orientações fornecidas pelos enfermeiros às pessoas com DM sobre o cuidado com os pés; investigar a frequência da realização do exame dos pés e os aspectos avaliados; verificar quais atividades de educação em saúde são realizadas pelos enfermeiros para as pessoas com DM. Métodos: estudo descritivo com abordagem quantitativa, realizado com 38 enfermeiros da Estratégia Saúde da Família, através de um questionário, durante maio a julho/2013. Os dados foram analisados com base na estatística descritiva. Estudo aprovado pelo CEP do CCS/UFPB, CAEE nº 03459112.1.0000.5188. Resultados: constatou-se que 26 (68,4%) enfermeiros orientam quanto ao uso de calçados confortáveis; 19 (50,0%) enfermeiros avaliam os pelos e as unhas mensalmente; 12 (31,6%) enfermeiros realizam orientações como atividade de educação em saúde. Conclusão: a atuação dos enfermeiros da Estratégia Saúde da Família contribui na prevenção do pé diabético, proporcionando a realização do autocuidado. Descritores: Enfermagem, Diabetes Mellitus, Pé diabético, Educação em saúde.
Investigation on enhancement of weld strength between PMMA and PBT in laser transmission welding—Using intermediate material
Authors: Wang Xiao , Zhong Xuejiao , Liu Wei , Liu Baoguang , Liu Huixia 
Published: July 2016

ABSTRACTPoly(methyl methacrylate) (PMMA) and poly(butylene terephthalate) (PBT) are widely used in industry; however, poor compatibility between two materials lead to poor weld strength. Polycarbonate (PC) has good compatibility with PMMA and PBT. Therefore, the welding method was that PC film as intermediate material was used to enhance weld strength in laser transmission welding (LTW) of PMMA and PBT. Through the LTW experiment, the weld strength was tested by mechanical testing and it was found that the best weld strength was improved more than four times than the weld strength without intermediate material. By observing the micro morphology of the weld zone, one reason was founded that the bubbles can be used to form micro‐mechanical riveting to enhance the weld strength. The reptation time for PMMA, PC, and PBT were investigated to analyze the establishment of the weld strength. When the reptation time is much shorter than time in molten state, the higher weld strength is feasible. It can be concluded that the weld strength of PC/PBT was higher than the weld strength of PMMA/PC. The equilibrium interfacial width was calculated through Helfand's theory to analyze the compatibility of dissimilar materials. The equilibrium interfacial width for PMMA/PC and PC/PBT were similar to tube diameter. That is the reason for weld strength enhancement. And then, the response surface methodology was designed to predict the weld strength.© 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44167.
An Algorithm for Accurate Distributed Time Synchronization in Mobile Wireless Sensor Networks from Noisy Difference Measurements
Authors: Liao Chenda , Barooah Prabir 
Published: July 2016

AbstractWe propose a distributed algorithm for time synchronization in mobile wireless sensor networks. The problem of time synchronization is formulated as nodes estimating their skews and offsets from noisy difference measurements of offsets and logarithm of skews; the measurements acquired by time‐stamped message exchanges between neighbors. The algorithm ensures that the estimation error is mean square convergent (variance converging to 0) under certain conditions. A sequence of scheduled update instants is used to meet the requirement of decreasing time‐varying gains that need to be synchronized across nodes with unsynchronized clocks. Moreover, a modification on the algorithm is also presented to improve the initial convergence speed. Simulations indicate that highly accurate global time estimates can be achieved with the proposed algorithm for long time durations, while the errors in competing algorithms increase over time.
Zum Tragverhalten der selbstregulierenden interaktiven Membrangründung
Authors: Detert Oliver , König Diethard , Alexiew Dimiter , Schanz Tom 
Published: July 2016

AbstractDer Beitrag berichtet über eine neuartige Gründungsmethode für Schütt‐ und Dammkörper, wie beispielsweise Rohstoffhalden oder Verkehrsdämme, auf gering tragfähigen Böden großer Mächtigkeit. Zur Analyse des Trag‐ und Verformungsverhaltens dieser „selbstregulierenden interaktiven Membrangründung” wurden Zentrifugen‐Modellversuche und umfangreiche numerische Berechnungen in Form von globalen Sensitivitätsanalysen und Parameterstudien durchgeführt. Deren Ergebnisse sowie der daraus abgeleitete Ansatz zur Dimensionierung werden im vorliegenden Beitrag vorgestellt. Eine Validierung des entwickelten Ansatzes erfolgt am Ende des Beitrags über eine Vergleichsrechnung mit dem numerischen Modell.
Horizon scanning the European bio‐based economy: a novel approach to the identification of barriers and key policy interventions from stakeholders in multiple sectors and regions
Authors: Hodgson Edward , Ruiz‐Molina María‐Eugenia , Marazza Diego , Pogrebnyakova Eugenia , Burns Caitlin , Higson Adrian , Rehberger Max , Hiete Michael , Gyalai‐Korpos Miklos , Lucia Lorenzo Di , Noël Yolande , Woods Jeremy , Gallagher Joe 
Published: July 2016

AbstractThere is international recognition that developing a climate‐smart bioeconomy is essential to the continuation of economic development, reduction of greenhouse gas emissions, and adaptation to climatic change; Bio‐based products have an important role in making this transition happen. Supporting policy interventions have been put forward at European and national levels to support innovation and development of bio‐based products and services. This study asks whether suggested policy interventions reflect the needs of stakeholders and examines how these needs vary between European regions. This consultation was performed through an online survey of 447 experts actively involved in bio‐based research, industry, and governance across Europe. The majority of responses received were from stakeholders in France, Germany, Italy, Spain, and the UK which are examined in greater depth.Climate change was clearly an important driver for bio‐based innovation as 86% of the respondents considered climate change to be a significant threat. There were clear differences between regions but also areas of consensus between stakeholders across the European regions surveyed. In particular there was consensus regarding the need for improved access to financial support and the need to ensure continuity of policy. The need to build investor confidence through demonstration of bio‐based technologies, the provision of greater clarity regarding best conversion routes for specific feedstocks, and the need to promote a culture of industrial symbiosis were also regarded as important interventions. © 2016 The Authors. Biofuels, Bioproducts and Biorefining published by Society of Chemical Industry and John Wiley & Sons, Ltd.
A colorimetric sensor for the selective detection of fluoride ions
Authors: Wan Chin‐Feng , Chir Jiun‐Ly , Wu An‐Tai 
Published: July 2016

AbstractA colorimetric receptor L was prepared. Receptor L can selectively sense F− based on distinct color changes among a series of ions. It can selectively sense F− through an intramolecular hydrogen bond interaction. A Job plot indicated a 1:1 complexation stoichiometry between receptor L and F−. The association constant for L–F− in CH3CN was determined as 9.70 × 104 M−1 using a Stern–Volmer plot.
Prognostic value of MMP9 activity level in resected stage I B lung adenocarcinoma
Authors: Yu Yongfeng , Ding Zhengping , Jian Hong , Shen Lan , Zhu Lei , Lu Shun 
Published: July 2016

AbstractThe clinical outcomes of patients with early‐stage non‐small cell lung cancer (NSCLC) have remained unsatisfactory after complete surgical resection. The objective of this study was to explore the prognostic value of matrix metalloproteinase 9 (MMP9) activity level in Chinese patients with stage I B lung adenocarcinoma. A sensitive and validated method was employed for determining the activity of MMP9 in human lung adenocarcinoma cells in vitro. Then, the association was examined between the level of MMP9 enzymatic activity and clinical outcomes. A total of 104 cases were stratified according to the IASLC/ATS/ERS classification scheme and activity of MMP9 was analyzed by SensoLyte® assay kit. The results showed that the MMP9 activity was the highest in solid predominant and micropapillary predominant subtypes, intermediate in acinar predominant and papillary predominant subtypes, and the lowest in lepidic predominant subtype. Multivariate analysis revealed that pathological subtype and activity of MMP9 were independent prognostic factors for disease‐free survival (DFS), respectively (P = 0.005 and 0.029). Significant relationship existed between enzyme activity of MMP9 and prognosis. And the 30 months DFS of high‐ and low‐level MMP9 activity tumors was 44.2% and 84.1% (P < 0.0001), respectively. High‐level MMP9 activity is correlated with aggressive tumor behaviors and poor clinical outcomes in early‐stage lung adenocarcinoma after complete resection.
Targeted TET oxidase activity through methyl‐CpG‐binding domain extensively suppresses cancer cell proliferation
Authors: Mizuguchi Yasuhiko , Saiki Yuriko , Horii Akira , Fukushige Shinichi 
Published: July 2016

AbstractDNA methyltransferase (DNMT) inhibitors are epigenetic drugs used to treat myelodysplastic syndrome. They not only induce DNA demethylation but also have significant cytostatic and cytotoxic effects; however, the relationships between these characteristics have not been established yet due to the lack of a method to induce only DNA demethylation. Herein, we show that a fusion protein comprised of the methyl‐CpG‐binding domain (MBD) and the catalytic domain of Ten‐eleven translocation protein 1 (TET1‐CD) globally demethylates and upregulates a number of methylated genes. These upregulated genes frequently contained CpG islands (CGIs) within ± 1000 bp of the transcription start site (TSS). Interestingly, 65% of the genes upregulated fivefold or more by MBD‐TET1‐CDwt were also reactivated after treatment with a DNMT inhibitor, 5‐azacytidine (Aza‐CR), suggesting that gene reactivation by both methods primarily shares the same mechanism, DNA demethylation. In order to examine whether DNA demethylation affects the growth of cancer cells, we have established a tetracycline inducible system that can regulate the expression of MBD‐TET1‐CDwt in a prostate cancer cell line, LNCaP. The induction of MBD‐TET1‐CDwt demethylated and upregulated glutathione S‐transferase pi 1 (GSTP1), one of the hypermethylated genes in prostate cancer. In accordance with the reactivation of methylated genes, induction of MBD‐TET1‐CDwt extensively suppressed the growth of LNCaP cells through G1/S arrest. These results clearly indicate that TET oxidase activity recruited at methyl‐CpG sites through MBD induces reactivation of hypermethylated genes by DNA demethylation and allows us to analyze the effect of only global DNA demethylation in a wide variety of cancer cells.