bluuebunny/crossref_metadata_2025_split huggingface dataset:
Totally minimally invasive two-stage esophagectomy with intrathoracic hand-sewn anastomosis: short-term clinical and oncological outcomes
Authors: Elshaer M , Gravante G , Tang C-B , Jayanthi N V 
Published: December 2017

SUMMARYSeveral esophageal resection techniques have been reported in literature. The objective of this study is to assess postoperative and oncological outcomes of two-stage minimally invasive esophagectomy (MIE) in a prone position using thoracoscopic hand-sewn anastomosis. Consecutive patients who underwent two-stage MIE in 2016 performed by the senior author were included. This was compared with the preceding cohort of consecutive patients who underwent two-stage hybrid esophagectomy (HE). The primary outcome was 30-day morbidity and mortality. The secondary outcomes were operation duration, length of stay (LOS), total nodes examined (TNE), number of positive nodes (NPN), and resection margin. Overall, 15 patients underwent MIE and 11 patients underwent HE. Respiratory complications occurred in three (20.0%) patients in the MIE group and in five (45.5%) patients in the HE group (P = 0.218). Cardiac complications occurred in two (18.2%) patients, and two other patients (18.2%) experienced anastomotic leak in the HE group. Mean operative duration was 349 ± 41.6 min in MIE and 309 ± 47.8 min in HE (P = 0.040). Median LOS was 10 days (range: 7–70) in MIE and 13 days (range: 10–116) in HE (P = 0.045). Median TNE was 23 (range: 12–36) in MIE and 20 (range: 14–47) in HE (P = 0.775). Longitudinal margin was involved in one patient (9.1%) in HE and no longitudinal margin was involved in the MIE group. Circumferential resection margin was involved in seven patients (46.7%) in MIE and in four patients (36.4%) in HE (P = 0.391). Two-stage MIE using hand-sewn technique is safe and feasible without compromising surgical and oncological outcomes. A multicenter large trial is recommended to confirm these results.
Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing
Authors: Fang Chao , Zhong Huanzi , Lin Yuxiang , Chen Bing , Han Mo , Ren Huahui , Lu Haorong , Luber Jacob M , Xia Min , Li Wangsheng , Stein Shayna , Xu Xun , Zhang Wenwei , Drmanac Radoje , Wang Jian , Yang Huanming , Hammarström Lennart , Kostic Aleksandar D , Kristiansen Karsten , Li Junhua 
Published: December 2017

Abstract

Background
More extensive use of metagenomic shotgun sequencing in microbiome research relies on the development of high-throughput, cost-effective sequencing. Here we present a comprehensive evaluation of the performance of the new high-throughput sequencing platform BGISEQ-500 for metagenomic shotgun sequencing and compare its performance with that of 2 Illumina platforms.

Findings
Using fecal samples from 20 healthy individuals, we evaluated the intra-platform reproducibility for metagenomic sequencing on the BGISEQ-500 platform in a setup comprising 8 library replicates and 8 sequencing replicates. Cross-platform consistency was evaluated by comparing 20 pairwise replicates on the BGISEQ-500 platform vs the Illumina HiSeq 2000 platform and the Illumina HiSeq 4000 platform. In addition, we compared the performance of the 2 Illumina platforms against each other. By a newly developed overall accuracy quality control method, an average of 82.45 million high-quality reads (96.06% of raw reads) per sample, with 90.56% of bases scoring Q30 and above, was obtained using the BGISEQ-500 platform. Quantitative analyses revealed extremely high reproducibility between BGISEQ-500 intra-platform replicates. Cross-platform replicates differed slightly more than intra-platform replicates, yet a high consistency was observed. Only a low percentage (2.02%–3.25%) of genes exhibited significant differences in relative abundance comparing the BGISEQ-500 and HiSeq platforms, with a bias toward genes with higher GC content being enriched on the HiSeq platforms.

Conclusions
Our study provides the first set of performance metrics for human gut metagenomic sequencing data using BGISEQ-500. The high accuracy and technical reproducibility confirm the applicability of the new platform for metagenomic studies, though caution is still warranted when combining metagenomic data from different platforms.
Flipping the Nursing Classroom Without Flipping Out the Students
Authors: Greenwood Victoria Anne , Mosca Caroline 
Published: December 2017

Abstract
Flipping the class has been touted as a method of increasing student participation and improving learning outcomes. In this study, nursing students were introduced to the flipped classroom. They were instructed to listen to narrated PowerPoint presentations before class and be ready to participate in case studies that would enhance their understanding of important concepts. When comparing student test scores before and after flipping the classroom, we found a statistically significant increase of 8.04 points after the change in instruction. Results support the hypothesis that a flipped classroom may improve learning outcomes.
Resource Allocation Strategies to Increase the Efficiency and Sustainability of Gavi’s Health System Strengthening Grants
Authors: Mimche Honoré , Squires Ellen , Miangotar Yodé , Mokdad Ali , El Bcheraoui Charbel 
Published: December 2017

Background:
Despite the increase in Health System Strengthening (HSS) grants, there is no consensus among global health actors about how to maximize the efficiency and sustainability of HSS programs and their resulting gains. To formally analyze and compare the efficiency and sustainability of Gavi’s HSS grants, we investigated the factors, events and root causes that increased the time and effort needed to implement HSS grants, decreased expected outcomes and threatened the continuity of activities and the sustainability of the results gained through these grants in Cameron and Chad.

Methods:
We conducted 2 retrospective independent evaluations of Gavi’s HSS support in Cameroon and Chad using a mixed methodology. We investigated the chain of events and situations that increased the effort and time required to implement the HSS programs, decreased the value of the funds spent and hindered the sustainability of the implemented activities and gains achieved.

Results:
Root causes affecting the efficiency and sustainability of HSS grants were common to Cameroon and Chad. Weaknesses in health workforce and leadership/governance of the health system in both countries led to interrupting the HSS grants, reprogramming them, almost doubling their implementation period, shifting their focus during implementation toward procurements and service provision, leaving both countries without solid exit plans to maintain the results gained.

Conclusions:
To increase the efficiency and sustainability of Gavi’s HSS grants, recipient countries need to consider health workforce and leadership/governance prior, or in parallel to strengthening other building blocks of their health systems.
Lidocaine Spray Versus Paracervical Block During Loop Electrosurgical Excision Procedure: A Randomized Trial
Authors: Limwatanapan Nopporn , Chalapati Wadwilai , Songthamwat Srisuda , Saenpoch Surapong , Buapaichit Kuanoon , Songthamwat Metha 
Published: December 2017

Objective
The aim of the study was to compare the effectiveness of pain control between lidocaine spray (LS) and paracervical block (PB) with lidocaine during the loop electrosurgical excision procedure (LEEP).

Materials and Methods
A single-blinded randomized controlled trial was conducted on 132 women who underwent LEEP of the cervix. The participants were randomly allocated to either a PB group or a LS group. The PB group participants were anesthetized by PB using 10 mL of 2% lidocaine with 1:100,000 of epinephrine. The LS group participants were locally anesthetized with four puffs (40 mg) of 10% LS, which was applied thoroughly to the cervix. Pain scores, using 10-cm visual analog scales, were obtained and compared for participants in both groups during anesthesia, during excision and 30 minutes after excision.

Results
A total of 132 LEEPs were performed with 66 in LS group and 66 in PB group. The mean (SD) pain scores during excision were 5.2 (2.4) in the LS group and 4.2 (3.3) in the PB group (mean difference = 1.1, 95% CI = 0.8 to 2.1, p = .033), which was within the nonclinically significant margin of this study. The baseline pain score after speculum examination was not significantly different in both groups. There was no adverse effect in the LS group compared with eight cases in the PB group (tinnitus, numbness, palpitation or tachycardia, and hypertension).

Conclusions
The local 40 mg of 10% LS can be used to substitute for PB for pain control during LEEP of the cervix. It also resulted in fewer adverse effects.
Discovery and characterization of coding and non-coding driver mutations in more than 2,500 whole cancer genomes
Authors: Rheinbay Esther , Nielsen Morten Muhlig , Abascal Federico , Tiao Grace , Hornshøj Henrik , Hess Julian M. , Pedersen Randi Istrup , Feuerbach Lars , Sabarinathan Radhakrishnan , Madsen Tobias , Kim Jaegil , Mularoni Loris , Shuai Shimin , Lanzós Andrés , Herrmann Carl , Maruvka Yosef E. , Shen Ciyue , Amin Samirkumar B. , Bertl Johanna , Dhingra Priyanka , Diamanti Klev , Gonzalez-Perez Abel , Guo Qianyun , Haradhvala Nicholas J. , Isaev Keren , Juul Malene , Komorowski Jan , Kumar Sushant , Lee Donghoon , Lochovsky Lucas , Liu Eric Minwei , Pich Oriol , Tamborero David , Umer Husen M. , Uusküla-Reimand Liis , Wadelius Claes , Wadi Lina , Zhang Jing , Boroevich Keith A. , Carlevaro-Fita Joana , Chakravarty Dimple , Chan Calvin W.Y. , Fonseca Nuno A. , Hamilton Mark P. , Hong Chen , Kahles Andre , Kim Youngwook , Lehmann Kjong-Van , Johnson Todd A. , Kahraman Abdullah , Park Keunchil , Saksena Gordon , Sieverling Lina , Sinnott-Armstrong Nicholas A. , Campbell Peter J. , Hobolth Asger , Kellis Manolis , Lawrence Michael S. , Raphael Ben , Rubin Mark A. , Sander Chris , Stein Lincoln , Stuart Josh , Tsunoda Tatsuhiko , Wheeler David A. , Johnson Rory , Reimand Jüri , Gerstein Mark B. , Khurana Ekta , López-Bigas Núria , Martincorena Iñigo , Pedersen Jakob Skou , Getz Gad 
Published: December 2017

AbstractDiscovery of cancer drivers has traditionally focused on the identification of protein-coding genes. Here we present a comprehensive analysis of putative cancer driver mutations in both protein-coding and non-coding genomic regions across >2,500 whole cancer genomes from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium. We developed a statistically rigorous strategy for combining significance levels from multiple driver discovery methods and demonstrate that the integrated results overcome limitations of individual methods. We combined this strategy with careful filtering and applied it to protein-coding genes, promoters, untranslated regions (UTRs), distal enhancers and non-coding RNAs. These analyses redefine the landscape of non-coding driver mutations in cancer genomes, confirming a few previously reported elements and raising doubts about others, while identifying novel candidate elements across 27 cancer types. Novel recurrent events were found in the promoters or 5’UTRs ofTP53, RFTN1, RNF34,andMTG2,in the 3’UTRs ofNFKBIZandTOB1,and in the non-coding RNARMRP.We provide evidence that the previously reported non-coding RNAsNEAT1andMALAT1may be subject to a localized mutational process. Perhaps the most striking finding is the relative paucity of point mutations driving cancer in non-coding genes and regulatory elements. Though we have limited power to discover infrequent non-coding drivers in individual cohorts, combined analysis of promoters of known cancer genes show little excess of mutations beyondTERT.
Comparative analysis of genetic diversity and differentiation of cauliflower (Brassica oleraceavar.botrytis) accessions from twoex situgenebanks
Authors: Yousef Eltohamy A. A. , Müller Thomas , Börner Andreas , Schmid Karl J. 
Published: December 2017

AbstractCauliflower (Brassica oleraceavar.botrytis) is an important vegetable crop for human nutrition. We characterized 192 cauliflower accessions from the USDA and IPK genebanks with genotyping by sequencing (GBS). They originated from 26 different countries and represent about 44% of all cauliflower accessions in both genebanks. The analysis of genetic diversity revealed that accessions formed two major groups that represented the two genebanks and were not related to the country of origin. This differentiation was robust with respect to the analysis methods that included principal component analysis, ADMIXTURE and neighbor-joining trees. Genetic diversity was higher in the USDA collection and significant phenotypic differences between the two genebanks were found in three out of six traits investigated. GBS data have a high proportion of missing data, but we observed that the exclusion of single nucleotide polymorphisms (SNPs) with missing data or the imputation of missing SNP alleles produced very similar results. The results indicate that the composition and type of accessions have a strong effect on the structure of genetic diversity ofex situcollections, although regeneration procedures and local adaptation to regeneration conditions may also contribute to a divergence.Fst-based outlier tests of genetic differentiation identified only a small proportion (<1%) of SNPs that are highly differentiated between the two genebanks, which indicates that selection during seed regeneration is not a major cause of differentiation between genebanks. Seed regeneration procedures of both genebanks do not result in different levels of genetic drift and loss of genetic variation. We therefore conclude that the composition and type of accessions mainly influence the level of genetic diversity and explain the strong genetic differentiation between the twoex situcollections. In summary, GBS is a useful method for characterizing genetic diversity in cauliflower genebank material and our results suggest that it may be useful to incorporate routine genotyping into accession management and seed regeneration to monitor the diversity present inex situcollections and to reduce the loss of genetic diversity during seed regeneration.
Accuracy and Repeatability of Trajectory Rod Measurement Using Laser Scanners
Authors: Liscio Eugene , Guryn Helen , Stoewner Daniella 
Published: December 2017

AbstractThree‐dimensional (3D) technologies contribute greatly to bullet trajectory analysis and shooting reconstruction. There are few papers which address the errors associated with utilizing laser scanning for bullet trajectory documentation. This study examined the accuracy and precision of laser scanning for documenting trajectory rods in drywall for angles between 25° and 90°. The inherent error range of 0.02°–2.10° was noted while the overall error for laser scanning ranged between 0.04° and 1.98°. The inter‐ and intraobserver errors for trajectory rod placement and virtual trajectory marking showed that the range of variation for rod placement was between 0.1°–1° in drywall and 0.05°–0.5° in plywood. Virtual trajectory marking accuracy tests showed that 75% of data values were below 0.91° and 0.61° on azimuth and vertical angles, respectively. In conclusion, many contributing factors affect bullet trajectory analysis, and the use of 3D technologies can aid in reduction of errors associated with documentation.
Accuracy and Reliability of Total Body Mass Estimation Techniques from Stature and Bi‐iliac Breadth in Non‐Hispanic U.S. Whites from the Bass Donated Skeletal Collection
Authors: Schaffer William C. , Dunn Tyler E. 
Published: December 2017

AbstractThis paper tests the fidelity of a recent method that used the NHANES III dataset as a proxy to estimate total body mass from stature and bi‐iliac breadth in U.S. White males and females. The bi‐iliac breadths of 230 males and 152 females identified as non‐Hispanic U.S. White from the Bass Donated Skeletal Collection were measured, and along with stature from predonor paperwork, total body mass estimates were calculated and then compared to body masses recorded on predonor paperwork. Male and female samples were subdivided by body mass index (BMI [kg/m2]) categories established by the World Health Organization. Our results suggest that total body mass estimates can be accurately assessed provided that the individual is within 18.50 ≤ BMI ≤ 29.99 for White males and 18.50 ≤ BMI ≤ 24.99 for White females. Recommendations on how to report total body mass estimates are also presented.
Coarse fibrillatory waves in atrial fibrillation predict success of electrical cardioversion
Authors: Zhao Tian X. , Martin Claire A. , Cooper John P. , Gajendragadkar Parag R. 
Published: December 2017

BackgroundTo determine whether the presence of “coarse” fibrillatory waves (Fw) seen on surface ECGs of patients with persistent atrial fibrillation (AF) predict maintenance of sinus rhythm (SR) at 6 weeks after electrical cardioversion (ECV).MethodsPreprocedure ECGs from 94 consecutive patients with persistent AF scheduled to undergo ECV at a single centre were classified as having coarse Fw (≥0.1 mV) or fine Fw (<0.1 mV) in leads II or V1. The primary outcome was ECG rhythm at 6‐week clinical follow‐up. Demographic and echocardiographic data were also collected.ResultsThirty‐two patient ECGs (34%) had coarse Fw on baseline ECG in either or both leads II or V1 with no significant differences in baseline demographics compared to those patients with fine Fw. At 6 weeks post‐ECV, in the coarse Fw group 72% of patients maintained SR vs. 42% in the fine Fw group (χ2, p = .006) with the odds ratio (OR) of maintaining SR at 6 weeks in the presence of coarse Fw being 3.5 (95% CI: 1.4–8.9, p = .007). Across the overall study population, there were no other significant univariate predictors of SR at 6 weeks post‐ECV.ConclusionClassifying persistent AF using the maximal Fw amplitude on a surface ECG is a simple and reproducible method of predicting medium‐term success of ECV, independent of traditional risk factors.
Capsosiphon fulvescens extracts improve obesity‐associated metabolic disorders and hepatic steatosis in high‐fat diet‐induced obese mice
Authors: Yun Mi‐Young , Lee Jae‐Sug , Kim Byoung‐Soo , Choi Hwa‐Jung 
Published: December 2017

AbstractHerbal dietary supplements have attracted more and more attention owing to their relative effectiveness in obesity ‐related metabolic disorders and diseases. This study investigated the therapeutic effects and underlying mechanisms of Capsosiphon fulvescens (CF) extracts on obesity, their associated metabolic disorders and hepatic steatosis in high‐fat diet (HFD)‐induced obese mice. Male C57BL/6 mice were fed with normal, HFD/Vehicle and HFD/CF (orally 300 mg/kg/day for CF). After 12 weeks, CF blocked HFD‐induced body weight, food intake, liver weight, hepatic triglyceride (TG), fat mass (weight of abdominal subcutaneous fat and epididymal adipose tissue) and biochemical parameters (total cohlesterol, glucose, TG, creatinine, high‐density lipoproteins cholesterol and low‐density lipoprotein cholesterol) of serum. CF also had improved serum levels of adiponectin, leptin and insulin‐like growth factor‐1 in HFD/CF mice. Moreover, CF ameliorated the hepatic steatosis‐reducing size of white adipose tissue. These results indicate that CF have anti‐obesity effects and are effective for reducing metabolic risk and hepatic steatosis.
Structure and sensilla of the antennae and mouthparts of Loxocephala perpunctata Jacobi (Hemiptera: Fulgomorpha: Eurybrachidae)
Authors: Meng Yinfeng , Qin Daozheng 
Published: December 2017

AbstractThe ultramorphology of the antennae and mouthparts of the adult Loxocephala perpunctata Jacobi was studied through a scanning electron microscope. Seven types of sensilla were found on antennomeres, including a Böhm bristle on the scape, sensillum trichoideum and plaque organ on the pedicel, two subtypes of sensilla chaetica and two subtypes of sensilla campaniformia on these two antennomeres; and Bourgoin's organ with sensory pegs and sensilla basiconicum on the basal bulb of the flagellum. The mouthparts of L. perpunctata are of the typical piercing‐sucking type, similar to mouthparts found in other hemipteran insects. In general, six types of sensilla (i.e., four subtypes of sensilla chaetica, sensillum basiconicum, subapical labial sensillum, uniporous peg‐like sensillum, multiporous peg‐like sensillum and two subtypes of bristle‐like sensilla) were detected on different locations of the labium, with the last three, and numerous cuticular processes, present on the labial tip. The potential functions of these sensilla are discussed.
Three‐year follow‐up after psychotherapy for young people with bipolar disorder
Authors: Inder Maree L , Crowe Marie T , Moor Stephanie , Carter Janet D , Luty Suzanne E , Frampton Christopher M , Joyce Peter R 
Published: December 2017

Objectives(1) To examine the differences between interpersonal and social rhythm therapy (IPSRT) and specialist supportive care (SSC) in the longer term impacts of IPSRT and SSC on cumulative depression and mania symptoms over a further 78‐week follow‐up period post treatment.(2) To calculate the survival time before recurrence of a new mood episode over the 3‐year period.MethodsOne hundred young people with bipolar disorder aged between 15 and 36 years who had been randomized to treatment with either IPSRT or SSC for 78 weeks were followed up for a subsequent 78 weeks. The Longitudinal Interval Follow‐up Evaluation was completed at 26‐week intervals. A Mann‐Whitney U test was used to determine if there were significant differences between therapy types and a Kaplan‐Meier survival analysis was used to determine time to recurrence. Cox regression was used to assess the association between time to relapse and therapy type.ResultsThere were no significant differences between therapies at each of the data points for either depression or mania scores. The mean change in depression and mania in both groups was significantly different for all three follow‐up data points. The actuarial cumulative recurrence rates were 53% for IPSRT and 49% for SSC. There was no significant difference between the groups in time to recurrence.ConclusionsWhile there were no significant differences between the two therapies, there was an overall reduction in symptoms in both therapies. There may be sustained benefits in providing intensive psychotherapies in conjunction with pharmacotherapy for young people with bipolar disorder.