bluuebunny/crossref_metadata_2025_split huggingface dataset:
Internal Variability Dominated the Extreme Cold Wave Over North America in December 2022
Authors: Gong Hainan , Ma Kangjie , Wang Lin 
Published: September 2024

AbstractIn December 2022, North America experienced an unprecedented extreme cold event. However, the underlying physical mechanisms of this cold wave, and the extent to which it is driven by internal variability or external forcing, are not fully understood. Using ERA5 reanalysis data and the HadGEM3‐A‐N216 attribution simulations, we identified internal variability as the main cause, contributing −5.14 K to surface air temperature (SAT) anomalies in North America. External forcing slightly mitigated the cold by 0.42 K. An internally generated wave train from the North Pacific, influenced in combination by Pacific‐North American (PNA) and North Pacific Oscillation (NPO) teleconnection patterns, initiated this intense cyclonic event, contributing −2.18 K and −2.12 K to SAT anomalies, respectively. La Niña‐like sea surface temperature anomalies amplified this wave train and resultant cold wave. Additionally, excessive snow cover in the previous November also intensified the December cold anomalies by enhancing surface albedo and reducing solar radiation.
Explaining the Evolution of Ion Velocity Distributions at a Low Activity Comet
Authors: Moeslinger A. , Gunell H. , Nilsson H. , Fatemi S. , Stenberg Wieser G. 
Published: September 2024

AbstractAt a low activity comet the plasma is distributed in an asymmetric way. The hybrid simulation code Amitis is used to look at the spatial evolution of ion velocity distribution functions (VDFs), from the upstream solar wind (SW) to within the comet magnetosphere where the SW is heavily mass‐loaded by the cometary plasma. We find that the spatial structures of the ions and fields form a highly asymmetric induced magnetosphere. The VDFs of SW and cometary ions vary drastically for different locations in the comet magnetosphere. The shape of the VDFs differ for different species. The SW protons show high anisotropies that occasionally resemble partial rings, in particular at small cometocentric distances. A second, decoupled, proton population is also found. Solar wind alpha particles show similar anisotropies, although less pronounced and at different spatial scales. The VDFs of cometary ions are mostly determined by the structure of the electric field. We perform supplementary dynamic particle backtracing to understand the flow patterns of SW ions that lead to these anisotropic distributions. This tracing is needed to understand the origin of cometary ions in a given part of the comet magnetosphere. The particle tracing also aids in interpreting observed VDFs and relating them to spatial features in the electric and magnetic fields of the comet environment.
Efficient combinatorial adaptor-mediated targeting of acute myeloid leukemia with CAR T-cells
Authors: Volta Laura , Myburgh Renier , Pellegrino Christian , Koch Christian , Maurer Monique , Manfredi Francesco , Hofstetter Mara , Kaiser Anne , Schneiter Florin , Müller Jan , Buehler Marco M. , De Luca Roberto , Favalli Nicholas , Magnani Chiara F. , Schroeder Timm , Neri Dario , Manz Markus G. 
Published: September 2024

AbstractCAR T-cell products targeting lineage-specific cell-of-origin antigens, thereby eliminating both tumor and healthy counterpart cells, are currently clinically approved therapeutics in B- and plasma-cell malignancies. While they represent a major clinical improvement, they are still limited in terms of efficacy by e.g. single, sometimes low-expressed antigen targeting, and in terms of safety by e.g., lack of on-off activity. Successful cell-of-origin non-discriminative targeting of heterogeneous hematopoietic stem and progenitor cell malignancies, such as acute myeloid leukemia (AML), will require antigen-versatile targeting and off-switching of effectors in order to then allow rescue by hematopoietic stem cell transplantation (HSCT), preventing permanent myeloablation. To address this, we developed adaptor-CAR (AdFITC-CAR) T-cells targeting fluoresceinated AML antigen-binding diabody adaptors. This platform enables the use of adaptors matching the AML-antigen-expression profile and conditional activity modulation. Combining adaptors significantly improved lysis of AML cells in vitro. In therapeutic xenogeneic mouse models, AdFITC-CAR T-cells co-administered with single diabody adaptors were as efficient as direct CAR T-cells, and combinatorial use of adaptors further enhanced therapeutic efficacy against both, cell lines and primary AML. Collectively, this study provides proof-of-concept that AdFITC-CAR T-cells and combinations of adaptors can efficiently enhance immune-targeting of AML.
Interleukin 6 and interleukin 17A serum levels and gene- polymorphisms in the development of early allograft rejection in living donor liver transplant recipients
Authors: Awad Samah Mohammed , El Batanony Eman Helmy , Elmahdy Shaimaa K. , Allam Esraa Tawfik , Rizk Sara Kamal , Zaid Ahmed B. , Taha Mohammad , Salem Radwa H. 
Published: September 2024

AbstractThe aim of this study is to evaluate the role of serum level of Interleukin 6(IL-6) and Interleukin 17 (IL-17) in liver transplantation outcome for living recipients, Analyze the relation between the gene polymorphism and the occurrence of rejection after liver transplantation and Study the relation between the gene polymorphism and the occurrence of different infectious complications. The study was conducted in March 2023 and included 60 healthy volunteers from the National Liver Institute (NLI) blood bank at Menoufia University and 120 live donation liver recipient patients at NLI. During one month of liver transplantation, the cytokine levels (IL-17, IL-6 proteins, IL-6 G-174C, and IL-17 A rs2275913 gene polymorphism) and CD4 levels for 60 patients of 120 live donation liver recipient patients whom early reject transplanted tissue and the same parameters were measured after 6 months follow up for non-reject group. The main finding of this study was that the post-transplant rejection group and the post-transplant non-rejection and control groups differed significantly in the genotype frequency (CC, CG, and GG) or alleles of IL-6 G-174C (p = 0.011). On the other hand IL-17A rs2275913 gene polymorphism and its alleles (p = 0.71) showed no statistically significant difference. We also observed that serum IL-17 levels, with 100% specificity and 100% sensitivity threshold, will be more sensitive and specific than serum IL-6 and CD4 count in differentiating post-transplant rejection from non-rejection patients. The results showed that there was no significant relationship between the genotypes and serum levels of interleukins and the type and degree of rejection. Proinflammatory cytokines might be useful indicators for distinguishing and early identifying unfavorable outcomes after transplantation, allowing for prompt and effective treatment intervention. To evaluate these findings, prospective clinical trials are required.
Long-term application of FYM and fertilizer N improve soil fertility and enzyme activity in 51st wheat cycle under pearl millet-wheat
Authors: Sheoran Sunita , Prakash Dhram , Yadav Parmod Kumar , Gupta Rajeev Kumar , Al-Ansari Nadhir , El-Hendawy Salah , Mattar Mohamed A. 
Published: September 2024

AbstractOur study from an ongoing research experiment initiated in Rabi 1967 at the Research Farm of CCS Haryana Agricultural University, Haryana, India, reports that during the 51st wheat cycle in pearl millet-wheat sequence, adding FYM in both seasons significantly impacted various soil parameters at different wheat growth stages compared to the rabi season. The application of 15 t of FYM ha−1 resulted in a considerable increase in dissolved organic carbon content (9.1–11.2%), available P (9.7–12.1%), and available S (12.6–17.1%), DHA levels by 7.3–22.0%, urease activity (10.1 and 17.0%), β-Glucosidase activity (6.2–8.4%), and APA activity (5.2–10.6%), compared to 10 t FYM ha−1. Application of N120 exhibited a considerable improvement in DHA (11.0–23.2%), β-Glucosidase (9.4–19.2%), urease (13.3–28.3%), and APA (3.3–6.2%) activity compared to control (N0). At stage 3, the box plot revealed that 50% of the available N, P, and S values varied from 223.1 to 287.9 kg ha−1, 53.0 to 98.2 kg ha−1, and 50.0 to 97.6 kg ha−1, respectively. Principal component analysis, with PC1 explaining 94.7% and PC2 explaining 3.15% of the overall variability, and SOC had a polynomial relationship with soil characteristics (R2 = 0.89 to 0.99). Applying FYM15 × N120 treatment during both seasons proved beneficial in sustaining the health of sandy loam soil in North-West India.
A comparative guide to expression systems for phage lysin production
Authors: Cremelie Emma , Vázquez Roberto , Briers Yves 
Published: September 2024

Abstract
Phage lysins, bacteriophage-encoded enzymes tasked with degrading their host’s cell wall, are increasingly investigated and engineered as novel antibacterials across diverse applications. Their rapid action, tuneable specificity, and low likelihood of resistance development make them particularly interesting. Despite numerous application-focused lysin studies, the art of their recombinant production remains relatively undiscussed. Here, we provide an overview of the available expression systems for phage lysin production and discuss key considerations guiding the choice of a suitable recombinant host. We systematically surveyed recent literature to evaluate the hosts used in the lysin field and cover various recombinant systems, including the well-known bacterial host Escherichia coli or yeast Saccharomyces cerevisiae, as well as plant, mammalian, and cell-free systems. Careful analysis of the limited studies expressing lysins in various hosts suggests a host-dependent effect on activity. Nonetheless, the multitude of available expression systems should be further leveraged to accommodate the growing interest in phage lysins and their expanding range of applications.
Sulfation pathways in the maintenance of functional beta-cell mass and implications for diabetes
Authors: Mueller Jonathan Wolf , Thomas Patricia , Dalgaard Louise Torp , da Silva Xavier Gabriela 
Published: September 2024

Abstract
Diabetes Type 1 and Type 2 are widely occurring diseases. In spite of a vast amount of biomedical literature about diabetic processes in general, links to certain biological processes are only becoming evident these days. One such area of biology is the sulfation of small molecules, such as steroid hormones or metabolites from the gastrointestinal tract, as well as larger biomolecules, such as proteins and proteoglycans. Thus, modulating the physicochemical propensities of the different sulfate acceptors, resulting in enhanced solubility, expedited circulatory transit, or enhanced macromolecular interaction. This review lists evidence for the involvement of sulfation pathways in the maintenance of functional pancreatic beta-cell mass and the implications for diabetes, grouped into various classes of sulfated biomolecule. Complex heparan sulfates might play a role in the development and maintenance of beta-cells. The sulfolipids sulfatide and sulfo-cholesterol might contribute to beta-cell health. In beta-cells, there are only very few proteins with confirmed sulfation on some tyrosine residues, with the IRS4 molecule being one of them. Sulfated steroid hormones, such as estradiol-sulfate and vitamin-D-sulfate, may facilitate downstream steroid signaling in beta-cells, following de-sulfation. Indoxyl sulfate is a metabolite from the intestine, that causes kidney damage, contributing to diabetic kidney disease. Finally, from a technological perspective, there is heparan sulfate, heparin, and chondroitin sulfate, that all might be involved in next-generation beta-cell transplantation. Sulfation pathways may play a role in pancreatic beta-cells through multiple mechanisms. A more coherent understanding of sulfation pathways in diabetes will facilitate discussion and guide future research.
Upfront Combined Hydroxyurea and Imatinib versus Imatinib Monotherapy in Newly Diagnosed Chronic Phase Chronic Myeloid Leukemia: A Randomized Controlled Trial
Authors: Chetia Rituparna , Palepu Sarika , Dutta Vikramjeet , Bandyopadhyay Arkapal , Mathew Anisha , Vaniyath Sudeep , Bakliwal Anamika , Chattopadhyay Debranjani , Rajoreya Ashok , Dhamija Puneet , Naithani Manisha , Singh Neha , Nath Uttam Kumar 
Published: September 2024

Background Tyrosine kinase inhibitors like imatinib have become the cornerstone of therapy in chronic phase-chronic myeloid leukemia (CML-CP). However, the role of hydroxyurea (HU), a deoxyribonucleic acid synthesis inhibitor, has been less explored in the disease. Hence, the present study was conducted to compare the efficacy of structured dose of HU based on baseline total leukocyte count (TLC) with imatinib in CML patients.
Method An open-label randomized controlled trial was conducted in 90 newly diagnosed CML-CP patients, aged ≥ 18 years. Patients were randomized to receive either baseline leucocyte count-based structured dose of HU with imatinib or imatinib monotherapy for 3 months. Quantitative real-time polymerase chain reaction for BCR-ABL1 to assess early molecular response (EMR) and safety evaluation according to the Common Terminology Criteria for Adverse Events version 5 was done.
Results Median age of patients was 36.5 years (36 [interquartile range [IQR]: 30–45] in I-HU, 38 [IQR: 31–47] in imatinib monotherapy) with male predominance. Fatigue was the most common symptom at diagnosis. Splenomegaly was seen in 89% (median spleen size: 10 [IQR: 6–15] cm). At 3 months, complete hematological response was seen in 74 patients (36 in I-HU, 38 in imatinib monotherapy). Overall, 68 patients achieved EMR (34 in I-HU, 34 in imatinib monotherapy, p = 0.53). The most common hematological toxicity, anemia, was seen in 80 patients (41 in I-HU, 39 in imatinib monotherapy). In 37 patients, nonhematological toxicities seen were nausea and vomiting (20 in I-HU, 17 in imatinib monotherapy). No dose limiting toxicities were reported.
Conclusion Addition of upfront TLC-based dosing of HU to imatinib was not found to significantly improve the hematological response and EMR at 3 months. However, long-term studies with a larger sample size with structured dose of HU can be undertaken as it forms a preferred adjunctive therapy for initial, rapid cytoreduction in hyperviscosity or leukostasis-related symptoms in patients of CML.
Intensive Oral Hygiene Interventions during Therapy of Acute Leukemia May Result in Detrimental Outcomes: A Randomized Clinical Trial
Authors: Dubashi Biswajit , Mote Nirmal Pratap , Krishnan B. , Kayal Smita , Kumar K.T. Harichandra , Abirami M. , Devi Nirmala , Ganesan Prasanth , Nisha Yadav 
Published: September 2024

Objectives There are no standard guidelines on oro-dental care during induction therapy of acute leukemia (AL). This study aimed to assess the effect of intensive oral hygiene practice on oral mucositis, infection, and disease outcomes compared to standard clinical practice.
Materials and Methods Newly diagnosed patients with AL were randomized to receive either standard oral hygiene protocol (group A, n = 92) or comprehensive oral hygiene protocol (group B, n = 91). In group A, the oral hygiene indexes were measured by the dentist at baseline and at the end of treatment. In group B, weekly monitoring of oral hygiene indexes by the dentist and interventions in the form of oral cavity inspection, probing for gum health, and use of a soft toothbrush and education on oral hygiene practices were carried out.
Results The frequency of mucositis was higher in group B (60%) than in group A (40%; p = 0.09). There was no difference in the median Simplified Oral Hygiene Index (OHI-S; 0.5 vs. 0.6) and Silness and Loe plaque index (0.4 vs. 0.25) between the groups. The local (11 vs. 1%; p = 0.005) and systemic infection rate (82.2 vs. 65.2%; p = 0.009) were higher in group B than in group A.
Conclusion This study failed to show the superiority of a comprehensive oral hygiene protocol compared to standard protocol in reducing oral mucositis in patients receiving induction therapy for AL. We hypothesize that frequent intervention in the oral cavity may lead to the dissemination of infection.
Pathology of the Proximal Radius in Juvenile Pugs
Authors: Obel Christina , Rondahl Veronica , Rohdin Cecilia , Friling Lisa , Bergström Annika 
Published: September 2024

AbstractThe aim of this study was to describe the physeal pathology of the proximal radius in four skeletally immature male Pugs. Physical examination, radiography, and computed tomography (CT) of the thoracic limbs were performed in all four dogs. Two Pugs were available for long-term follow-up and two Pugs were euthanatized after the diagnostic imaging results. Four male Pugs, aged between 6 and 7 months, were presented with a history of thoracic limb lameness lasting 2 to 3 months. Radiography and CT of the thoracic limbs showed irregularity of the proximal radial physes with varying degrees of radiolucency within the adjacent metaphyses. The pathology was associated with elbow joint incongruity and angular deformity of the antebrachium. Two dogs were euthanatized, one of which was autopsied, and histopathology of the proximal radius revealed bilateral physeal dysplasia. At long-term follow-up of the two surviving dogs, lameness had resolved. Radiography and CT scans of the thoracic limbs revealed normal bone opacity within the proximal radius and resolution of the radiolucent areas of the proximal radial physes. However, incongruity of the radioulnar joint remained. In this report, the resolution of identified physeal pathology at the proximal radius in juvenile Pugs demonstrates the potential for spontaneous remission despite the presence of notable radiographic changes.
Design and simulation of a Ka-band frequency doubling gyroklystron amplifier
Authors: Singh Abhijeet , Chauhan Madan Singh , Srivastava Neelam 
Published: September 2024

The design methodology of a Ka-band frequency doubling two cavity gyroklystron has been discussed in this paper. In this study, the amplifier’s design and simulation are accomplished by employing nonlinear analysis and validated using particle-in-cell simulation techniques. The output cavity functions in the TE02 mode at two times the input signal frequency at the second harmonic of the cyclotron frequency, while the input cavity is powered by a 17.5 GHz source and functions in the TE01 mode at the first harmonic of the cyclotron resonance. The device’s performance has been examined both in the presence and in the absence of a beam. A number of factors were taken into account in order to optimize the interaction structure dimension to reach the device’s targeted performance. By adjusting the structural features and electron beam parameters, the device’s RF output power is assessed in an effort to increase efficiency. Simulation results estimate that the device displays a gain of 21.5 dB, an efficiency of 21.6%, a power of 227 kW, and a −3 dB bandwidth of ∼0.3%.
Temperature dependent self-assembly of lanthanide coordination polymers based on a benzimidazolium dicarboxylate linker: synthesis, structure and luminescence properties
Authors: You Li-Xin , Wang Wen-Yu , Wang Xiao-Juan , Xiong Gang , Wang Shu-Ju , Ding Fu , Liang Jin-Guang , Sun Ya-Guang 
Published: September 2024

Five new coordination polymers, namely [Ln(L)(NO3)2]n (Ln = La (1), Pr (2) and Sm (3)), [Eu(L)(NO3)2·1.5H2O]n (4) and [Tb(L)(OAc)2(H2O)·3H2O]n (5) (HOAc = CH3COOH), were synthesised by reaction of lanthanide nitrates with 1,3-bis(4-carboxybenzyl)benzimidazolium chloride (H2L+Cl−) under solvothermal conditions. All the coordination polymers were characterised by powder X-ray diffraction, infrared spectroscopy, thermogravimetric analysis, elemental analysis and single crystal X-ray diffraction. Complexes 1–5 exhibit two different 2-D structures due to the variation in reaction temperature. Complexes 1–4 are isostructural and crystallised in the monoclinic system, C2/c space group, showing a two-dimensional layer with Ln chains linked by L− ligands. Complex 5 crystallised in the P21/n space group, featuring a 2-D layer different from 1–4 with Tb dimers linked by L− ligands through carboxylates. Further, the luminescence properties of complexes 3–5 were investigated. In addition, the fluorescence lifetime and quantum efficiency of complex 4 were measured.
The deflection angle and quasi-periodic oscillations of an extended gravitational decoupled black hole solution
Authors: Mushtaq Farzan , Tiecheng Xia , Ditta Allah , Mustafa G , Maurya S K 
Published: September 2024

Abstract
In weak field limits, we compute the deflection angle of a gravitational decoupling extended black hole (BH) solution. We obtained the Gaussian optical curvature by examining the null geodesic equations with the help of Gauss–Bonnet theorem (GBT). We also looked into the deflection angle of light by a black hole in weak field limits with the use of the Gibbons–Werner method. We verify the graphical behavior of the black hole after determining the deflection angle of light. Additionally, in the presence of the plasma medium, we also determine the deflection angle of the light and examine its graphical behavior. Furthermore, we compute the Einstein ring via gravitational decoupling extended black hole solution. We also compute the quasi-periodic oscillations and discuss their graphical behavior.
Rapid hologram generation through backward ray tracing and adaptive-resolution wavefront recording plane
Authors: Zhu 朱 Jianying 建英 , Bi 毕 Yong 勇 , Sun 孙 Minyuan 敏远 , Gao 高 Weinan 伟男 
Published: September 2024

Abstract
An advanced method for rapidly computing holograms of large three-dimensional (3D) objects combines backward ray tracing with adaptive resolution wavefront recording plane (WRP) and adaptive angular spectrum propagation. In the initial phase, a WRP with adjustable resolution and sampling interval based on the object’s size is defined to capture detailed information from large 3D objects. The second phase employs an adaptive angular spectrum method (ASM) to efficiently compute the propagation from the large-sized WRP to the small-sized computer-generated hologram (CGH). The computation process is accelerated using CUDA and OptiX. Optical experiments confirm that the algorithm can generate high-quality holograms with shadow and occlusion effects at a resolution of 1024 × 1024 in 29 ms.