bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein Aggregate‐Ligand Binding Assays Based on Microfluidic Diffusional Separation
Authors: Zhang Yingbo , Buell Alexander K. , Müller Thomas , De Genst Erwin , Benesch Justin , Dobson Christopher M. , Knowles Tuomas P. J. 
Published: August 2016

AbstractThe measurement of molecular interactions with pathological protein aggregates, including amyloid fibrils, is of central importance in the context of the development of diagnostic and therapeutic strategies against protein misfolding disorders. Probing such interactions by conventional methods can, however, be challenging because of the supramolecular nature of protein aggregates, their heterogeneity, and their often dynamic nature. Here we demonstrate that direct measurement of diffusion on a microfluidic platform enables the determination of affinity and kinetics data for ligand binding to amyloid fibrils in solution. This method yields rapid binding information from only microlitres of sample, and is therefore a powerful technique for identifying and characterising molecular species with potential therapeutic or diagnostic application.
3 D Imaging and Structural Analysis of a Mesoporous‐Silica‐Body‐Supported Eggshell Cobalt Catalyst for Fischer–Tropsch Synthesis
Authors: Han Ting , Li Xiaopeng , Lin Chao , Zhang Haojie , Gao Peng , Zhao Yonghui , Du Fuping , Chen Yuyun , Sun Yuhan 
Published: August 2016

AbstractFor understanding the relationship between the structure and catalytic property and further optimizing the synthetic steps, it is imperative to obtain basic information about the characteristics of heterogeneous catalysts, such as porosity, dispersion, and accessibility of the active metal phases. Herein, we reported a multiscale analysis of an eggshell‐Co‐based catalyst for the Fischer–Tropsch synthesis (FTS), which was prepared by wetness impregnation. Conventional characterization techniques, such as mercury porosimetry, nitrogen physisorption, and transmission electron microscopy (TEM), were employed for obtaining basic information about the porosity and dispersion of cobalt on the nanoscale; in addition, phase‐contrast X‐ray computed microtomography (X‐ray μCT) as a powerful method was employed for obtaining vitally complementary information regarding the 3 D structure of a millimeter‐sized mesoporous silica support and a full‐field view of the macroscopic distribution of cobalt. The Co reduction behavior and FTS performance was correlated with the structure analyses, highlighting the importance of simultaneously controlling the dispersion and spatial distribution of Co ranging from macro to nanoscale.
Designing ‘Totem’ C2‐Symmetrical Iron Porphyrin Catalysts for Stereoselective Cyclopropanations
Authors: Carminati Daniela Maria , Intrieri Daniela , Caselli Alessandro , Le Gac Stéphane , Boitrel Bernard , Toma Lucio , Legnani Laura , Gallo Emma 
Published: August 2016

AbstractThe catalytic activity of the iron(III) C2 chiral porphyrin Fe(2)(OMe) in alkene cyclopropanation is herein reported. The catalyst promoted the reaction of differently substituted styrenes with diazo derivatives with trans‐diastereoselectivities and enantioselectivities up to 99:1 and 87 %, respectively. In addition, high TON and TOF values (up to 10 000 and 120 000 h−1, respectively) were observed indicating good activity and stability of the catalyst in optimized experimental conditions. The study of the cyclopropanation reaction revealed that the porphyrin skeleton is composed of two ‘totem’ parts which were independently responsible for the observed enantio‐ and diastereoselectivities. To further our research we also investigated the catalytic role of the methoxy axial ligand coordinated to the iron atom. The molecular structure of Fe(2)(OMe) was optimized by DFT calculations which were also employed to achieve a better understanding of the mechanistic details of the carbene transfer reaction.
Redox and Acid–Base Properties of Binuclear 4‐Terphenyldithiophenolate Complexes of Nickel
Authors: Koch Felix , Berkefeld Andreas , Schubert Hartmut , Grauer Claudius 
Published: August 2016

AbstractThis work reports on the redox and acid–base properties of binuclear complexes of nickel from 1,4‐terphenyldithiophenol ligands. The results provide insight into the cooperative electronic interaction between a dinickel core and its ligand. Donor/acceptor contributions flexibly adjust to stabilize different redox states at the metals, which is relevant for redox reactions like proton reduction. Proton transfer to the [S2Ni2] core and Ni−H bond formation are kinetically favored over the thermodynamically favored yet unproductive proton transfer to ligand.
Role of Metal Oxide Electron‐Transport Layer Modification on the Stability of High Performing Perovskite Solar Cells
Authors: Singh Trilok , Singh Jai , Miyasaka Tsutomu 
Published: August 2016

AbstractOrganic–inorganic hybrid perovskite light absorbers have recently emerged as a “holy grail” for next generation thin‐film photovoltaics with excellent optoelectronics properties and low fabrication cost. In a very short span of time, we have witnessed a pronounced and unexpected progress in organic– inorganic perovskite solar cells (PSCs) with a vertical rise in power conversion efficiency from 3.8 to 22.1 %. In this manuscript we focus specifically on the recent development of metal oxide‐based electron‐transporting layer (ETL) modification for high performing PSCs and their stability. This review highlights various methodologies to modify existing compact/scaffold layers for improving device performance and stability. Various aspects of the ETL are discussed with different metal oxide compact layers in their relation to modification in mesoporous layers towards the design of a cell structure with high performance and stability.
Metabolic adaptations in a range‐expanding arthropod
Authors: Van Petegem Katrien H. P. , Renault David , Stoks Robby , Bonte Dries 
Published: August 2016

AbstractDespite an increasing number of studies documenting life‐history evolution during range expansions or shifts, we lack a mechanistic understanding of the underlying physiological processes. In this explorative study, we used a metabolomics approach to study physiological changes associated with the recent range expansion of the two‐spotted spider mite (Tetranychus urticae). Mite populations were sampled along a latitudinal gradient from range core to edge and reared under benign common garden conditions for two generations. Using gas chromatography–mass spectrometry, we obtained metabolic population profiles, which showed a gradual differentiation along the latitudinal gradient, indicating (epi)genetic changes in the metabolome in association with range expansion. These changes seemed not related with shifts in the mites’ energetic metabolism, but rather with differential use of amino acids. Particularly, more dispersive northern populations showed lowered concentrations of several essential and nonessential amino acids, suggesting a potential downregulation of metabolic pathways associated with protein synthesis.
Plasticity of fertilization rates under varying temperature in the broadcast spawning mussel,Mytilus galloprovincialis
Authors: Eads Angela R. , Evans Jonathan P. , Kennington Winn Jason 
Published: August 2016

AbstractOceans are a huge sink for the increased heat associated with anthropogenic climate change, and it is vital to understand the heat tolerance of marine organisms at all life stages to accurately predict species’ responses. In broadcast spawning marine invertebrates, reproduction is a vulnerable process in which sperm and eggs are released directly into the open water. Gametes are then exposed to fluctuating environmental conditions that may impact their fertilizing capacity. Using the broadcast spawning Mediterranean mussel,Mytilus galloprovincialis,as a model species, we performed blocks of factorial mating crosses to assess the variance in fertilization rates among individuals under both ambient and elevated temperatures. Overall, we found a small, but significant decline in fertilization rates with elevated temperatures. However, there was substantial plasticity in responses, with particular mussels having increased fertilization under elevated temperatures, although the majority showed decreased fertilization rates. Our results suggest possible future reproductive costs to ocean warming inM. galloprovincialis, although it is also possible that genetic variation for thermal sensitivity may allow for adaptation to changing environmental conditions.
Formation of cholesterol oxides in lipid medium during microwave heating
Authors: Leal‐Castañeda Everth J. , Hernández‐Becerra Josafat A. , Rodríguez‐Estrada Maria Teresa , García Hugo S. 
Published: August 2016

Microwave (MW) heating of foods may cause the formation of cholesterol oxidation products (COPs). COPs formation depends on the conditions of MW heating and the composition of the food matrix. The aim of this work was to study the formation of major COPs from cholesterol incorporated into palm, extra virgin olive, soybean, or fish oils during MW heating, and to monitor changes in peroxide value and fatty acid (FA) profiles. Model systems composed of mixtures of cholesterol and oil (2.5 mg of cholesterol/g oil), were heated for 20 min in multimode (900 W), unimode MW (≈300 W) ovens, or convection oven at 180°C. Maximum total COPs contents largely varied (46.4–250.4 µg/g lipids), depending on the type of heating system and oil matrix. Multimode MW heating caused greater COPs formation than unimode MW. COPs formation trends in the conventional oven were similar to those of MW heating, except for fish oil. Results indicated that soybean oil, compared with the other oils tested, did not promote cholesterol oxidation during MW heating, while the opposite trend was observed for extra‐virgin olive oil and palm oil. FA profile and natural antioxidants influenced cholesterol and surrounding media oxidation during MW heating.Practical applications: This work explores the formation of oxysterols by microwave heating in model systems. This will help assess the possible risks for formation of these compounds during industrial and home cooking practices.Microwave (MW) heating of foods may cause formation of cholesterol oxidation products (COPs). In this work, a model system consisting of four types of edible oils with added cholesterol was employed and heated with three different forms (unimodal and multimodal MW, and conventional heating) for different time periods. Generation of COPs in all the model systems was monitored.
Comparison of phenolic content and antioxidant activities of millet varieties grown in different locations in Sri Lanka
Authors: Kumari Disna , Madhujith Terrence , Chandrasekara Anoma 
Published: August 2016

AbstractSoluble and bound phenolic compounds were extracted from different varieties of millet types namely, finger millet, foxtail, and proso millet cultivated at dry and intermediate climatic zones in Sri Lanka. The extracts were examined for their total phenolic content (TPC), total flavonoid content (TFC), and proanthocyanidin content (PC). The antioxidant activities were meassured by reducing power (RP), trolox equivalent antioxidant capacity (TEAC), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion chelating ability (FICA), and using a β carotene linoleate model system. The ferulic acid content of extracts were determined using high‐performance liquid chromatoghraphy (HPLC). Finger millet showed the highest phenolic content and antioxidant activities compared to proso and foxtail millets. The phenolic content as well as antioxidant activites of soluble and bound phenolic extracts of millets were affected by variety and cultivated location. The highest phenolic content and antioxidant activites were reported for millet samples cultivated in areas belonging to the dry zone in Sri Lanka.
Analysis of Genomewide DNA Methylation Reveals Differences in DNA Methylation Levels between Dormant and Naturally as well as Artificially Potentiated Pedicle Periosteum of Sika Deer (Cervus nippon)
Authors: YANG CHUN , LU XIAO , SUN HONGMEI , CHU WEN HUI , LI CHUNYI 
Published: August 2016

ABSTRACTDeer antlers are the only mammalian appendages that can fully regenerate each year from the permanent bony protuberances of the frontal bones, called pedicles. Pedicle periosteum (PP) is the key tissue for antler regeneration and the source of antler stem cells. The distal one third of the PP has acquired the ability to regenerate antlers and is termed the potentiated PP (PPP), whereas the proximal two thirds of the PP requires further interactions within its niche to launch antler regeneration and is termed the dormant PP (DPP). However, the molecular mechanisms underlying the process of potentiation from the DPP to the PPP are unknown. In this study, we used the fluorescence‐labeled methylation‐sensitive amplified polymorphism method to assess the levels of DNA methylation in both cells and tissues of the PPP and the DPP. The results showed that the levels of DNA methylation were significantly lower in the PPP compared to the DPP (P < 0.05). Therefore, DNA demethylation may be involved in the process of this potentiation. This involvement was further confirmed by functional testing by artificially creating a potentiated PP (aPPP) from DPP tissue. Moreover, we identified 15 methylated fragments by the methylation sensitive amplified polymorphism method that are either unique to the PPP or the DPP, which were further confirmed by Southern blot analysis. Taken together, our data suggest that DNA demethylation is involved in the process of PP potentiation, which is a prerequisite step for the initiation of antler regeneration. These findings provide the first experimental evidence to link epigenetic regulation and mammalian appendage regeneration.
Comparative lever analysis and ontogenetic scaling in esocid fishes: Functional demands and constraints in feeding biomechanics
Authors: Goulet Courtney L. , Smith Harrison J. , Maie Takashi 
Published: August 2016

ABSTRACTWhen animals grow, the functional demands that they experience often change as a consequence of their increasing body size. In this study, we examined the feeding biomechanics in esocid species that represent different size classes (small, Esox americanus; intermediate, Esox niger; large, Esox lucius), and how their bite forces and associated functional variables change as they grow. In order to evaluate bite performance through ontogeny, we dissected and measured dimensions of the feeding apparatus and the adductor mandibulae muscle complex with its segmentum facialis subdivisions such as the ricto‐malaris, stegalis and endoricto‐malaris across a wide range of body sizes. The collected morphological data was used as input variables for a published anatomical model to simulate jaw function in these fish species. Maximum bite forces for both anterior bite and posterior bite increased in isometry in E. americanus and E. niger. The posterior bite of E. lucius also increases in isometry, however, the anterior bite increases in positive allometry. Intraspecific comparison within E. lucius indicated the increase of bite forces in more developed individuals accelerated after the fish grew out of fingerling stage. In addition, our analysis indicated functional differentiation between subdivisions of the adductor mandibulae segmentum facialis, as well as interspecific differences in the pattern of contribution to the bite performance by these subdivisions. Our study provides insights into not only the musculoskeletal basis of the jaw function of esocid species, but also the feeding capacity of this species in relation to the functional demands it faces as one of the top predators in lake and river systems. J. Morphol. 277:1447–1458, 2016. © 2016 Wiley Periodicals, Inc.
Evaluation of the fossil fish‐specific diversity in a chadian continental assemblage: Exploration of morphological continuous variation in Synodontis (Ostariophysi, Siluriformes)
Authors: Pinton Aurélie , Le Fur Soizic , Otero Olga 
Published: August 2016

ABSTRACTIn the fossil record, the quantification of continuous morphological variation has become a central issue when dealing with species identification and speciation. In this context, fossil taxa with living representatives hold great promise, because of the potential to characterise patterns of intraspecific morphological variation in extant species prior to any interpretation in the fossil record. The vast majority of catfish families fulfil this prerequisite, as most of them are represented by extant genera. However, although they constitute a major fish group in terms of distribution, and ecological and taxonomic diversity, the quantitative study of their past morphological variation has been neglected, as fossil specimens are generally identified based on the scarcest remains, that is, complete neurocrania that bear discrete characters. Consequently, a part of freshwater catfish history is unprospected and unknown. In this study, we explored the morphological continuous variation of the humeral plate shape in Synodontis catfishes using Elliptic Fourier Analysis (EFA), and compared extant members and fossil counterparts. We analysed 153 extant specimens of 11 Synodontis species present in the Chad basin, in addition to 23 fossil specimens from the Chadian fossiliferous area of Toros Menalla which is dated around 7 Ma. This highly speciose genus, which is one of the most diversified in Africa, exhibits a rich fossil record with several hundred remains mostly identified as Synodontis sp. The analysis of the outline of the humeral plate reveals that some living morphological types were already represented in the Chad Basin 7 My ago, and allows for the discovery of extinct species. Beside illuminating the complex Neogene evolutionary history of Synodontis, these results underline the interest in the ability of isolated remains to reconstruct a past dynamic history and to validate the relevance of EFA as a tool to explore specific diversity through time. J. Morphol. 277:1486–1496, 2016. © 2016 Wiley Periodicals, Inc.
Spherical Polyolefin Particles from Olefin Polymerization in the Confined Geometry of Porous Hollow Silica Particles
Authors: Freudensprung Ines , Joe Daejune , Nietzel Sven , Vollmer Doris , Klapper Markus , Müllen Klaus 
Published: August 2016

Porous hollow silica particles (HSPs) are presented as new templates to control the product morphology in metallocene‐catalyzed olefin polymerization. By selectively immobilizing catalysts inside the micrometer‐sized porous hollow silica particles, the high hydraulic forces resulting from polymer growth within the confined geometries of the HSPs cause its supporting shell to break up from the inside. As the shape of the support is replicated during olefin polymerization, perfectly spherical product particles with very narrow size distribution can be achieved by using HSPs exhibiting a monomodal size distribution. Furthermore, the size of the obtained product particles can be controlled not only by the polymerization time but also by the size of the support material.
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Mesh‐free models for static analysis of smart laminated composite beams
Authors: Ray M. C. 
Published: August 2016

SummaryThis paper is concerned with the development of mesh‐free models for the static analysis of smart laminated composite beams. The overall smart composite beam is composed of a laminated substrate composite beam and a piezoelectric layer attached partially or fully at the top surface of the substrate beam. The piezoelectric layer acts as the distributed actuator layer of the smart beam. A layer‐wise displacement theory and an equivalent single‐layer theory have been used to derive the models. Several cross‐ply substrate beams are considered for presenting the numerical results. The responses of the smart composite beams computed by the present new mesh‐free model based on the layer‐wise displacement theory excellently match with those obtained by the exact solutions. The mesh‐free model based on the equivalent single‐layer theory cannot accurately compute the responses due to transverse actuation by the piezoelectric actuator. The models derived here suggest that the mesh‐free method can be efficiently used for the numerical analysis of smart structures. Copyright © 2016 John Wiley & Sons, Ltd.
Olfactomedin 1 negatively regulates NF‐κB signalling and suppresses the growth and metastasis of colorectal cancer cells
Authors: Shi Wei , Ye Zhihua , Zhuang Li , Li Yingchang , Shuai Wendi , Zuo Zhixiang , Mao Xueli , Liu Ranyi , Wu Jiangxue , Chen Shuai , Huang Wenlin 
Published: August 2016

AbstractUncontrolled growth and distant metastasis are hallmarks of colorectal cancer (CRC), but the mechanisms are poorly understood. Olfactomedin 1 (OLFM1), a member of the olfactomedin domain‐containing protein family, plays an important role in the development of neurogenic tissues. Recently, OLFM1 deregulation was frequently observed in several cancers, and it was induced in colon cell lines after treatment with the demethylating agent 5‐aza‐2'‐deoxycytidine. However, the function of OLFM1 in CRC remains unknown. In this study, we reanalysed published microarray data and found that OLFM1 was significantly down‐regulated in primary CRC samples compared to adjacent non‐cancerous tissues. The results of immunohistochemistry indicated that decreased OLFM1 expression was significantly associated with lymph node status (p = 0.023), distant metastasis (p < 0.001), and AJCC/TNM stage (p = 0.013), and CRC patients with low OLFM1 expression had consistently poor overall survival (OS; p < 0.001) and progression‐free survival (PFS; p < 0.001). Further analysis demonstrated that OLFM1 was epigenetically silenced in CRC tissues and cell lines via promoter hypermethylation. Overexpression and knockdown of OLFM1 attenuated and increased, respectively, CRC cells' proliferation, migration, and invasion in vitro and metastasis to the lung and liver in vivo. Mechanistically, the promotion of growth and metastasis of CRC cells by silencing of OLFM1 was associated with the activation of the non‐canonical NF‐κB signalling pathway. OLFM1 interacted with NF‐κB‐inducing kinase (NIK; MAP3K14) and repressed the phosphorylation of its downstream substrate Ikappa B kinase alpha (IKKα). OLFM1 expression was negatively correlated with the phosphorylation level of IKKα in CRC tissue samples. Knockdown of NIK impaired the ability of OLFM1 to repress NF‐κB signalling, cell growth or migration. Thus, OLFM1 may be a valuable biomarker and therapeutic target for CRC patients. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.