bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Characterization of a Bladeless Air Compressor
Authors: Tiwari Ravi Nath , Reggio Federico , Ferrari Mario Luigi , De Paepe Ward , Traverso Alberto 
Published: September 2024

Abstract
The Tesla compressor is an innovative technology that offers a unique approach to fluid compression. Unlike traditional compressors that use rotating blades, bladeless compressors utilize closely spaced disks to create compression. The purpose of this article is to design a prototype Tesla air compressor with optimal design parameters and investigate the performance and loss characteristics based on numerical analysis and experimental demonstration. The prototype model has been numerically investigated at different rotational speeds, and the results have been compared with those obtained in experiments. Computational fluid dynamics (CFD) simulations indicate that the rotor-only efficiency is greater than 90% at very low mass flowrates, while the coupling of the rotor and volute leads to a total-to-static efficiency of approximately 58% (without losses) at 14 g/s. At a nominal mass flow of 4 g/s, the highest total-to-static pressure ratio would be around 1.27. Experimental results indicate leakage losses greatly reduce net mass flow, while pressure ratio values are in good agreement with CFD predictions. During this experiment, a maximum isentropic efficiency of 32.4% is measured. Indeed, the prototype included ventilation and leakage losses, which were not modeled in the CFD analysis. It is remarkable that the compressor does not show any unstable behavior down to zero mass flow (closed valve test), where the CFD and the experiment show consistent pressure ratios. An estimation of the losses from end-wall friction and leakage flow is carried out using numerical simulations at different exit radial clearances. Increasing radial clearance results in an increase in leakage and end-wall power loss, the latter being driven mainly by the axial clearance with the casing, which remained unchanged. To minimize leakage, a Teflon ring has been used as a first measure. Numerical calculations have indicated that the leakage rate is approximately 6 g/s at design speed. A brush seal-type solution can improve the sealing system to reduce leakage.
Insights Into Turbocharger Centrifugal Compressor Stability Using High-Fidelity Computational Fluid Dynamics and a Novel Impeller Diffusion Factor
Authors: Liu Yiming , Spence Stephen , Horsley John , Mann Stuart , Stuart Charles 
Published: September 2024

Abstract
Turbocharger technologies are widely applied in the modern automotive industry to meet increasingly stringent carbon emission standards. To support the continuous enhancement of these tactics, delivering improvement to the performance and stability of turbocharger compressors at low mass flowrate regimes is of paramount importance. This chapter investigates the stability of a turbocharger compressor stage equipped with a ported shroud, using high-fidelity computational fluid dynamics (CFD) simulations. It was found that the recirculated flow passing through the ported shroud device had primary influence on the flow condition in the vicinity of the impeller tip region (80–100% span). This discovery facilitated the definition of a modified impeller diffusion factor (DF), which was applied to the CFD results for three different compressor geometry configurations to lead to the provision of quantitative metrics for impeller stability. Furthermore, the observed change in the limiting impeller DF along the compressor surge line indicated that the dominant components governing the instability of the entire stage had switched, i.e., the stalling behavior of the compressor stage had transitioned from impeller dominated stall to vaneless diffuser dominated stall. Additionally, the limiting impeller DF clearly showed that one of the compressor configurations with a change in the hub endwall recess around the rear of the impeller improved the impeller stability by 3%. By comparison, another configuration with a change to strut geometry in the ported shroud recirculation channel reduced the impeller stability by 10%. The present work addressed the gap in the understanding about the stability of different compressor configurations equipped with a ported shroud, and was useful for determining the changes in compressor global and local instability through a quantitative parameter based on a modified definition of DF.
Challenges and opportunities of phage therapy for
Klebsiella pneumoniae
infections
Authors: Kou Xin , Yang Xiaoyu , Zheng Rui 
Published: September 2024

ABSTRACT

Traditional antibiotics have been effective in many cases. However, the rise in multidrug-resistant bacteria has diminished their therapeutic efficacy, signaling the dawn of an era beyond antibiotics. The challenge of multidrug resistance in
Klebsiella pneumoniae
is particularly critical, with increasing global mortality and resistance rates. Therefore, the development of alternative therapies to antibiotics is urgently needed. Phages, which are natural predators of bacteria, have inherent advantages. However, comprehensive information on
K. pneumoniae
phages is lacking in current literature. This review aims to analyze and summarize relevant studies, focusing on the present state of phage therapy for
K. pneumoniae
infections. This includes an examination of treatment methodologies, associated challenges, strategies, new phage technologies, clinical trial safety and efficacy, regulatory issues, and future directions for phage therapy development. Enhancing phage technology is crucial for addressing the evolving threat of multidrug-resistant
K. pneumoniae
.
The human gut microbiome in health and disease: time for a new chapter?
Authors: Lee Jee-Yon , Bays Derek J. , Savage Hannah P. , Bäumler Andreas J. 
Published: September 2024

ABSTRACT

The gut microbiome, composed of the colonic microbiota and their host environment, is important for many aspects of human health. A gut microbiome imbalance (gut dysbiosis) is associated with major causes of human morbidity and mortality. Despite the central part our gut microbiome plays in health and disease, mechanisms that maintain homeostasis and properties that demarcate dysbiosis remain largely undefined. Here we discuss that sorting taxa into meaningful ecological units reveals that the availability of respiratory electron acceptors, such as oxygen, in the host environment has a dominant influence on gut microbiome health. During homeostasis, host functions that limit the diffusion of oxygen into the colonic lumen shelter a microbial community dominated by primary fermenters from atmospheric oxygen. In turn, primary fermenters break down unabsorbed nutrients into fermentation products that support host nutrition. This symbiotic relationship is disrupted when host functions that limit the luminal availability of host-derived electron acceptors become weakened. The resulting changes in the host environment drive alterations in the microbiota composition, which feature an elevated abundance of facultatively anaerobic microbes. Thus, the part of the gut microbiome that becomes imbalanced during dysbiosis is the host environment, whereas changes in the microbiota composition are secondary to this underlying cause. This shift in our understanding of dysbiosis provides a novel starting point for therapeutic strategies to restore microbiome health. Such strategies can either target the microbes through metabolism-based editing or strengthen the host functions that control their environment.
Autoproteolytic mechanism of CdiA toxin release reconstituted
in vitro
Authors: Tiu Ana Katrina Y. , Conroy Grace C. , Bobst Cedric E. , Hagan Christine L. 
Published: September 2024

ABSTRACT

Contact-dependent inhibition (CDI) is a mechanism of interbacterial competition in Gram-negative bacteria. Bacteria that contain CDI systems produce a large, filamentous protein, CdiA, on their cell surfaces. CdiA contains a C-terminal toxin domain that is transported across the outer membranes (OMs) of neighboring bacteria. Once inside a target bacterium, the toxin is released from the CdiA protein via a proteolytic mechanism that has not been well characterized. We have developed an
in vitro
assay to monitor this toxin release process and have identified several conserved amino acids that play critical roles in the autocatalytic mechanism. Our results indicate that a hydrophobic, membrane-like environment is required for CdiA to fold, and the proteolysis occurs through an asparagine cyclization mechanism. Our
in vitro
assay thus provides a starting point for analyzing the conformational state of the CdiA protein when it is inserted into a target cell’s OM and engaged in transporting the toxin across that membrane.

IMPORTANCE
It is challenging to develop new antibiotics capable of killing Gram-negative bacteria because their outer membranes are impermeable to many small molecules. Some Gram-negative bacteria, however, deliver much larger protein toxins through the outer membranes of competing bacteria in their environments using contact-dependent inhibition (CDI) systems. How these toxins traverse the outer membranes of their targets is not well understood. We have therefore developed a method to study the toxin delivery process in a highly simplified system using a fragment of a CDI protein. Our results indicate that the CDI protein assembles into a structure in the target membrane that catalyzes the release of the toxin. This CDI protein fragment enables further studies of the toxin delivery mechanism.
Early extensional salt tectonics controls deep-water sediment dispersal
Authors: Oppo Davide , Jackson Christopher A.-L. , Maselli Vittorio 
Published: September 2024

Abstract
Predicting the distribution of sedimentary facies during the early stages of deformation of salt-detached continental margins is key to constraining the location and stratigraphic architecture of hydrocarbon and CO2 reservoirs, as well as to understanding the oceanic carbon cycle. Despite its importance, we still have a relatively poor understanding of salt-sediment interactions during the early phases of extensional salt tectonics, mainly because subsequent salt-related deformation and/or deep burial of the related stratigraphic succession means the related deposits are poorly imaged in seismic reflection data and/or not penetrated by borehole data. We investigated the interplay between early extension-related salt deformation and deep-water sediment dispersal using 3-D seismic reflection data from the northern Levant Basin offshore Lebanon. Our results indicate that salt tectonics has two contrasting impacts: Whereas slope-parallel faults favor early sediment transfer along downslope-oriented corridors to the abyssal plain, slope-normal faults and ramp-syncline basins trap land-derived sediments, hampering or delaying their transport to the abyssal plain. These results help refine source-to-sink models of turbidite systems developing in young salt basins, highlighting the crucial role of extensional tectonics in controlling sediment dispersal and the development of intra-slope depocenters and emphasizing the impact of fault strike, ramp-syncline basin evolution, and salt thinning. Our study has significant implications for predicting the location of deep-water coarse-grained sediment and the preservation of land-derived organic carbon in mature, more structurally complex, salt basins.
Sarin exposure, mortality and cancer incidence in UK military veterans involved in human experiments at Porton Down: 52-year follow-up
Authors: Archer Gemma , Keegan Thomas , Wessely Simon , Venables Katherine M , Fear Nicola T 
Published: September 2024

ObjectivesWe investigated whether UK military personnel exposed to sarin during the ‘Service Volunteer Programme’ at Porton Down had increased rates of mortality or cancer incidence over a 52-year follow-up.MethodsA historical cohort study assembled from UK military records, comprising male veterans exposed to sarin during the ‘Service Volunteer Programme’ at Porton Down, UK (n=2975) and a comparison group of similar veterans who did not attend (n=2919). Mortality and cancer incidence data were obtained from national registries up to December 2019. Analysis was conducted using Cox regression adjusted for age, year of birth and service characteristics.ResultsOver a median follow-up of 52.2 years (range 2 days to 74.6 years), 1598 (53.7%) sarin-exposed veterans and 1583 (54.3%) non-exposed veterans died. Adjusted HRs for all-cause mortality were raised for any sarin exposure (HR=1.08, 95% CI 1.01 to 1.16), two or more exposures (HR=1.25, 95% CI 1.04 to 1.49) and higher doses (air >15 mg.min/m3) (HR=1.15, 95% CI 1.02 to 1.30). For cause-specific mortality, sarin exposure was associated with deaths from ‘other’ circulatory diseases (excludes ischaemic and cerebrovascular diseases) (HR=1.41, 95% CI 1.06 to 1.87) and alcohol-attributable deaths (HR=2.66, 95% CI 1.40 to 5.07). There was no association between sarin exposure and overall cancer incidence (HR=1.01, 95% CI 0.93 to 1.10), but cancer incidence was higher for alcohol-related neoplasms (HR=1.24, 95% CI 1.01 to 1.51).ConclusionsSarin exposure was associated with increased rates of mortality over a 50-year follow-up. The strongest associations were observed for deaths attributable to alcohol and ‘other’ circulatory diseases.
The Neurophysiological Costs of Learning in a Noisy Classroom: An Ecological Virtual Reality Study
Authors: Levy Orel , Korisky Adi , Zvilichovsky Yair , Zion Golumbic Elana 
Published: September 2024

Abstract
Many real-life situations can be extremely noisy, which makes it difficult to understand what people say. Here, we introduce a novel audiovisual virtual reality experimental platform to study the behavioral and neurophysiological consequences of background noise on processing continuous speech in highly realistic environments. We focus on a context where the ability to understand speech is particularly important: the classroom. Participants (n = 32) experienced sitting in a virtual reality classroom and were told to pay attention to a virtual teacher giving a lecture. Trials were either quiet or contained background construction noise, emitted from outside the classroom window. Two realistic types of noise were used: continuous drilling and intermittent air hammers. Alongside behavioral outcomes, we measured several neurophysiological metrics, including neural activity (EEG), eye-gaze and skin conductance (galvanic skin response). Our results confirm the detrimental effect of background noise. Construction noise, and particularly intermittent noise, was associated with reduced behavioral performance, reduced neural tracking of the teacher's speech and an increase in skin conductance, although it did not have a significant effect on alpha-band oscillations or eye-gaze patterns. These results demonstrate the neurophysiological costs of learning in noisy environments and emphasize the role of temporal dynamics in speech-in-noise perception. The finding that intermittent noise was more disruptive than continuous noise supports a “habituation” rather than “glimpsing” hypothesis of speech-in-noise processing. These results also underscore the importance of increasing the ecologically relevance of neuroscientific research and considering acoustic, temporal, and semantic features of realistic stimuli as well as the cognitive demands of real-life environments.
Lesion Mapping of the Spelling System's Central Cognitive Functions
Authors: Purcell Jeremy , Wiley Robert , Shea Jennifer , Rosenberg Samuel , Martin Randi , Rapp Brenda 
Published: September 2024

Abstract
The goal of this study was to improve our understanding of the central cognitive functions of spelling: orthographic long-term memory, phoneme-to-grapheme conversion, and orthographic working memory. To do so, we present a methodological innovation to support vector regression lesion-symptom mapping, which uses a deficit spectrum index to identify brain areas associated with one of each pair of spelling deficits. Using this approach, we find evidence of distinct neural substrates that are selectively associated with each of the three central spelling components, supporting a componential functional architecture of spelling. The specific findings provide neural evidence relevant to various debates regarding the nature of these key cognitive processes and their relationships, namely, questions concerning the degree to which phoneme-to-grapheme conversion is distinct from general phonological processing, the existence of distinct lexical and sublexical routes for translating sound to print, and whether or not working memory is distinct from long-term memory or embedded within it. A better understanding of these issues has clinical implications in terms of our understanding of dysgraphic deficits and approaches to intervention.
Virtual Voting in rfmos: A Procedural Odyssey at the Indian Ocean Tuna Commission
Authors: Schatz Valentin , Perraudeau Maia 
Published: September 2024

Abstract
This article examines, from the perspective of international institutional law, the variety of procedural problems which arose following an online vote by secret ballot taken by the Indian Ocean Tuna Commission (iotc) in 2021. By providing a detailed case-study of this novel instance of practice and the challenges it represents, this article aims to contribute to a better understanding of the institutional governance and procedural legal framework of regional fisheries management organizations (rfmo s). It first analyses the vote and the challenge to its validity. This article then constructs a taxonomy of the various procedural irregularities that were found to have occurred, the responsibility of institutional actors for their occurrence, and their legal consequences. The final substantive sections of this article examine how the iotc ultimately dealt with the situation and what potential role the available dispute settlement procedures could have had in addressing the discussed procedural irregularities.
An energy analysis methodology for residential heat pump retrofits
Authors: Day Tony , Morris Katherine , Chaer Issa , Gillich Aaron 
Published: September 2024

The government has targets to upscale domestic heat pump retrofit as part of the strategy to decarbonise heat. There are significant barriers to uptake including installation cost together with a lack of expertise in predicting and reporting energy and carbon performance. As a result, end user confidence in heat pumps can be low. This paper presents analysis of two monitored domestic properties using high resolution data. The aim is to develop reliable prediction and in-use diagnostic tools to improve transparency and reliability of heat pump performance. This paper focuses on a method drawn from theory as set out in CIBSE TM41, and shows how real building heat loss coefficients can be determined from regression analysis over different time frame resolutions. Practical application This research aims to provide residential heat pump retrofit providers with cost effective methods for assessing the suitability of dwellings to accept heat pump retrofit solutions, and to extend these techniques for the effective monitoring and performance evaluation of heat pumps post installation.
Ineffectiveness of 6,2′,4′-trimethoxyflavone in mitigating cerebral ischemia/reperfusion injury after post-reperfusion administration in rats
Authors: Woo Chul-Woong , Choi Monica Young , Heo Hwon , Chae Yeon Ji , Sung Yu Sub , Choi Yoonseok , Woo Dong Cheol 
Published: September 2024

Background Pharmacological inhibition of aryl hydrocarbon receptor (AhR) activation after ischemia alleviates cerebral ischemia/reperfusion (IR) injury. Purpose To investigate whether AhR antagonist administration after reperfusion was also effective in attenuating cerebral IR injury. Material and Methods A total of 24 Sprague–Dawley rats were divided into the sham-operated group (no IR), control group (IR), and 6,2′,4′-trimethoxyflavone (TMF) group (IR + TMF administration), with 10 rats assigned to each group. Cerebral IR injury was induced by 60 min of middle cerebral artery occlusion followed by reperfusion. TMF (5 mg/kg) was used as the AhR antagonist and was administered intraperitoneally immediately after reperfusion. Cerebral IR injury was observed using magnetic resonance imaging (MRI) and neurobehavioral assessments at baseline, immediately after ischemia, and at 3 days after ischemia. Results On MRI, the TMF group showed no significant differences in relative apparent diffusion coefficient (ADC), T2, and fractional anisotropy (FA) values; midline shift value; and infarct volume. In terms of neurobehavioral function, factors such as grip strength, contralateral forelimb use, time to touch, and time to remove adhesive tape from the forepaw, were also not significantly different between the control and TMF groups. Conclusion This study demonstrated that AhR treatment after reperfusion had no noticeable effect on reducing cerebral IR injury in rats.
Is tumour sequencing effective for the identification of germline BRCA1/2 pathogenic variant carriers?
Authors: Azzollini Jacopo , Capone Iolanda , Duca Matteo , Vingiani Andrea , Piccolo Alberta , Agnelli Luca , Tamborini Elena , Perrone Federica , Peissel Bernard , Lorenzini Daniele , Damian Silvia , Vernieri Claudio , Bianchi Giulia Valeria , Mantiero Mara , Ducceschi Monika , Polignano Maggie , Niger Monica , Nichetti Federico , Proto Claudia , Brambilla Marta , Colombo Elena , Stellato Marco , Conca Elena , Busico Adele , Manoukian Siranoush 
Published: September 2024

Introduction: Tumour BRCA1/2 sequencing has progressively increased along with the expanding indications for poly(ADP-ribose) polymerase inhibitors. In our study, we investigated the feasibility and outcomes of a workflow for the identification of germline carriers based on tumour sequencing results. Methods: Between April 2020 and December 2022, BRCA1/2 tumour testing results from 2020 patients were reviewed. Analysed tumours included: 323 ovarian, 104 breast, 314 pancreas-biliary, 87 prostate, 374 gastrointestinal, 309 lung, and 509 less common histologies. Testing was performed through small (only BRCA1/2, 16%) or comprehensive (>50 genes) next-generation sequencing panels (84%). Patients with pathogenic/likely pathogenic variants were referred for genetic counselling and germline testing. Results: Tumour BRCA1/2 pathogenic variants were identified in 145 patients (7%). The pathogenic variant frequency ranged between 23% (75/323 ovarian) and 3.5% (11/314 pancreas-biliary). The highest frequency was observed in high-grade ovarian carcinomas (27%, 64/235). By 30 June 2023, 79 out of 145 patients (54%) underwent subsequent genetic counselling and germline testing. In these patients, mostly affected with ovarian carcinoma (67%, 53/79), 48 were confirmed germline pathogenic variants (61%). Conclusions: In our tumour-to-germline testing approach, we observed the BRCA1/2 pathogenic variant frequency reported in other large unselected ovarian cancer cohorts, thus confirming its effectiveness in identifying putative germline carriers irrespective of eligibility for germline testing. As the range of tumours subjected to genetic testing broadens, this approach is expected to also be effective in other tumour settings for enhancing the identification of carriers, reducing the burden on genetic services, and avoiding unnecessary concerns related to germline testing.