bluuebunny/crossref_metadata_2025_split huggingface dataset:
CACNA1S expression in mouse retina: Novel isoforms and antibody cross-reactivity with GPR179
Authors: HASAN NAZARUL , RAY THOMAS A. , GREGG RONALD G. 
Published: June 2016

AbstractCacna1sencodes the α1S subunit (Cav1.1) of voltage-dependent calcium channels, and is required for normal skeletal and cardiac muscle function, where it couples with the ryanodine receptor to regulate muscle contraction. Recently CACNA1S was reported to be expressed on the tips of retinal depolarizing bipolar cells (DBCs) and colocalized with metabotropic glutamate receptor 6 (mGluR6), which is critical to DBC signal transduction. Further, in mGluR6 knockout mice, expression at this location is down regulated. We examined RNAseq data from mouse retina and found expression of a novel isoform ofCacna1s.To determine if CACNA1S was a functional component of the DBC signal transduction cascade, we performed immunohistochemistry to visualize its expression in several mouse lines that lack DBC function. Immunohistochemical staining with antibodies to CACNA1S show punctate labeling at the tips of DBCs in wild type (WT) retinas that are absent inGpr179nob5mutant retinas and decreased inGrm6−/−mouse retinas. CACNA1S and transient receptor potential cation channel, subfamily M, member 1 (TRPM1) staining also colocalized in WT retinas. Western blot analyses for CACNA1S of either retinal lysates or proteins after immunoprecipitation with the CACNA1S antibody failed to show the presence of bands expected for CACNA1S. Mass spectrometric analysis of CACNA1S immunoprecipitated proteins also failed to detect any peptides matching CACNA1S. Immunohistochemistry and western blotting after expression of GPR179 in HEK293T cells indicate that the CACNA1S antibody used here and in the retinal studies published to date, cross-reacts with GPR179. These data suggest caution should be exercised in conferring a role for CACNA1S in DBC signal transduction based solely on immunohistochemical staining.
Making the Ideal Real: Publicity and Morality in Kant
Authors: Zinkin Melissa 
Published: June 2016

AbstractThis article discusses the concept of publicity in Kant’s moral philosophy. Insofar as the concepts of ‘public’ and ‘private’ can describe our relations with others, they can be considered to be moral concepts. I argue that we can find in Kant a moral duty not to keep our maxims of action private, or secret. Whereas Korsgaard argues that sometimes in the face of evil it is permissible to sidestep the moral law, I argue that it is rather through publicity that we can deal with evil in the non-ideal world. Moreover, by being open with our maxims, moral progress is possible.
The Sacred and the Secular: Practical Applications of Water Rituals in the Ifugao Agricultural System
Authors: Acabado Stephen , Martin Marlon 
Published: June 2016

AbstractWater symbolisms permeate Ifugao religion, rituals, and oral tradition. Water plays a part in death, rebirth, and cleansing in Ifugao cosmology. As such, Ifugaos consider water as sacred. However, water is also central in Ifugao economy and politics. As a culture that highly values intensive wet-rice production in a mountain environment, managing access to water is necessary to maintain stability. Ifugao practices follow what Richard O'Connor described as the “agro-cultural complex” in which agricultural practices, social systems, and political, historical, and, cultural changes are understood as interlocking processes (O'Connor 1995). In this paper, we focus on the relationship between Ifugao water and agricultural rituals with the synchronizing and sequencing of agricultural activities. Using the concept of self-organization, we argue that water and agricultural rituals in Ifugao are not only meant to reinforce community cohesion, they also synchronize the farming activities crucial to a terraced ecology. Utilizing the practice ofpuntunaan(a ritual plot or parcel in the centre of an agricultural district) and the institution oftomona(the ritual leader of an agricultural district) as a case study, we observed that disruptions in the water and rice rituals stimulated great change in Ifugao sociopolitical organization.
aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis
Authors: Raman Renuka , Damle Indraneel , Rote Rahul , Banerjee Shamik , Dingare Chaitanya , Sonawane Mahendra 
Published: June 2016

AbstractEpithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis and have functions in mucous retention, membrane storage and abrasion resistance. It is largely unknown how the formation of these laterally long actin projections is regulated. Here, we show that antagonistic interactions between aPKC and Lgl–regulators of apical and basolateral domain identity, respectively,–control the length of microridges in the zebrafish periderm, the outermost layer of the epidermis. aPKC regulates the levels of Lgl and the active form of non-muscle myosinII at the apical cortex to prevent actin polymerization-dependent precocious fusion and elongation of microridges. Our data unravels the functional significance of exclusion of Lgl from the apical domain in epithelial cells.
Topological protection of multiparticle dissipative transport
Authors: Loehr Johannes , Loenne Michael , Ernst Adrian , de las Heras Daniel , Fischer Thomas M. 
Published: June 2016

AbstractTopological protection allows robust transport of localized phenomena such as quantum information, solitons and dislocations. The transport can be either dissipative or non-dissipative. Here, we experimentally demonstrate and theoretically explain the topologically protected dissipative motion of colloidal particles above a periodic hexagonal magnetic pattern. By driving the system with periodic modulation loops of an external and spatially homogeneous magnetic field, we achieve total control over the motion of diamagnetic and paramagnetic colloids. We can transport simultaneously and independently each type of colloid along any of the six crystallographic directions of the pattern via adiabatic or deterministic ratchet motion. Both types of motion are topologically protected. As an application, we implement an automatic topologically protected quality control of a chemical reaction between functionalized colloids. Our results are relevant to other systems with the same symmetry.
Geometrical assembly of ultrastable protein templates for nanomaterials
Authors: Glover Dominic J. , Giger Lars , Kim Steve S. , Naik Rajesh R. , Clark Douglas S. 
Published: June 2016

AbstractThe fabrication of nanoscale devices requires architectural templates on which to position functional molecules in complex arrangements. Protein scaffolds are particularly promising templates for nanomaterials due to inherent molecular recognition and self-assembly capabilities combined with genetically encoded functionalities. However, difficulties in engineering protein quaternary structure into stable and well-ordered shapes have hampered progress. Here we report the development of an ultrastable biomolecular construction kit for the assembly of filamentous proteins into geometrically defined templates of controllable size and symmetry. The strategy combines redesign of protein–protein interaction specificity with the creation of tunable connector proteins that govern the assembly and projection angles of the filaments. The functionality of these nanoarchitectures is illustrated by incorporation of nanoparticles at specific locations and orientations to create hybrid materials such as conductive nanowires. These new structural components facilitate the manufacturing of nanomaterials with diverse shapes and functional properties over a wide range of processing conditions.
Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice
Authors: Zhang Hongsheng , Kang Eunchai , Wang Yaqing , Yang Chaojuan , Yu Hui , Wang Qin , Chen Zheyu , Zhang Chen , Christian Kimberly M. , Song Hongjun , Ming Guo-li , Xu Zhiheng 
Published: June 2016

AbstractSeveral genome- and proteome-wide studies have associated transcription and translation changes of CRMP2 (collapsing response mediator protein 2) with psychiatric disorders, yet little is known about its function in the developing or adult mammalian brain in vivo. Here we show that brain-specific Crmp2 knockout (cKO) mice display molecular, cellular, structural and behavioural deficits, many of which are reminiscent of neural features and symptoms associated with schizophrenia. cKO mice exhibit enlarged ventricles and impaired social behaviour, locomotor activity, and learning and memory. Loss of Crmp2 in the hippocampus leads to reduced long-term potentiation, abnormal NMDA receptor composition, aberrant dendrite development and defective synapse formation in CA1 neurons. Furthermore, knockdown of crmp2 specifically in newborn neurons results in stage-dependent defects in their development during adult hippocampal neurogenesis. Our findings reveal a critical role for CRMP2 in neuronal plasticity, neural function and behavioural modulation in mice.
A simple two-state protein unfolds mechanically via multiple heterogeneous pathways at single-molecule resolution
Authors: Schönfelder Jörg , Perez-Jimenez Raul , Muñoz Victor 
Published: June 2016

AbstractA major drive in protein folding has been to develop experimental technologies to resolve the myriads of microscopic pathways and complex mechanisms that purportedly underlie simple two-state folding behaviour. This is key for cross-validating predictions from theory and modern computer simulations. Detecting such complexity experimentally has remained elusive even using methods with improved time, structural or single-molecule resolution. Here, we investigate the mechanical unfolding of cold shock protein B (Csp), a showcase two-state folder, using single-molecule force-spectroscopy. Under controlled-moderate pulling forces, the unfolding of Csp emerges as highly heterogeneous with trajectories ranging from single sweeps to different combinations of multiple long-lived mechanical intermediates that also vary in order of appearance. Steered molecular dynamics simulations closely reproduce the experimental observations, thus matching unfolding patterns with structural events. Our results provide a direct glimpse at the nanoscale complexity underlying two-state folding, and postulate these combined methods as unique tools for dissecting the mechanical unfolding mechanisms of such proteins.
Quasi 2D electronic states with high spin-polarization in centrosymmetric MoS2 bulk crystals
Authors: Gehlmann Mathias , Aguilera Irene , Bihlmayer Gustav , Młyńczak Ewa , Eschbach Markus , Döring Sven , Gospodarič Pika , Cramm Stefan , Kardynał Beata , Plucinski Lukasz , Blügel Stefan , Schneider Claus M. 
Published: June 2016

AbstractTime reversal dictates that nonmagnetic, centrosymmetric crystals cannot be spin-polarized as a whole. However, it has been recently shown that the electronic structure in these crystals can in fact show regions of high spin-polarization, as long as it is probed locally in real and in reciprocal space. In this article we present the first observation of this type of compensated polarization in MoS2 bulk crystals. Using spin- and angle-resolved photoemission spectroscopy (ARPES), we directly observed a spin-polarization of more than 65% for distinct valleys in the electronic band structure. By additionally evaluating the probing depth of our method, we find that these valence band states at the "Equation missing" point in the Brillouin zone are close to fully polarized for the individual atomic trilayers of MoS2, which is confirmed by our density functional theory calculations. Furthermore, we show that this spin-layer locking leads to the observation of highly spin-polarized bands in ARPES since these states are almost completely confined within two dimensions. Our findings prove that these highly desired properties of MoS2 can be accessed without thinning it down to the monolayer limit.
Novel strip-cast Mg/Al clad sheets with excellent tensile and interfacial bonding properties
Authors: Kim Jung-Su , Lee Dong Ho , Jung Seung-Pill , Lee Kwang Seok , Kim Ki Jong , Kim Hyoung Seop , Lee Byeong-Joo , Chang Young Won , Yuh Junhan , Lee Sunghak 
Published: June 2016

AbstractIn order to broaden industrial applications of Mg alloys, as lightest-weight metal alloys in practical uses, many efforts have been dedicated to manufacture various clad sheets which can complement inherent shortcomings of Mg alloys. Here, we present a new fabrication method of Mg/Al clad sheets by bonding thin Al alloy sheet on to Mg alloy melt during strip casting. In the as-strip-cast Mg/Al clad sheet, homogeneously distributed equi-axed dendrites existed in the Mg alloy side and two types of thin reaction layers, i.e., γ (Mg17Al12) and β (Mg2Al3) phases, were formed along the Mg/Al interface. After post-treatments (homogenization, warm rolling and annealing), the interfacial layers were deformed in a sawtooth shape by forming deformation bands in the Mg alloy and interfacial layers, which favorably led to dramatic improvement in tensile and interfacial bonding properties. This work presents new applications to multi-functional lightweight alloy sheets requiring excellent formability, surface quality and corrosion resistance as well as tensile and interfacial bonding properties.
Efficient scheme for hybrid teleportation via entangled coherent states in circuit quantum electrodynamics
Authors: Joo Jaewoo , Ginossar Eran 
Published: June 2016

AbstractWe propose a deterministic scheme for teleporting an unknown qubit state through continuous-variable entangled states in superconducting circuits. The qubit is a superconducting two-level system and the bipartite quantum channel is a microwave photonic entangled coherent state between two cavities. A Bell-type measurement performed on the hybrid state of solid and photonic states transfers a discrete-variable unknown electronic state to a continuous-variable photonic cat state in a cavity mode. In order to facilitate the implementation of such complex protocols we propose a design for reducing the self-Kerr nonlinearity in the cavity. The teleporation scheme enables quantum information processing operations with circuit-QED based on entangled coherent states. These include state verification and single-qubit operations with entangled coherent states. These are shown to be experimentally feasible with the state of the art superconducting circuits.
Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV
Authors: Onoyama Haruna , Kamiya Mako , Kuriki Yugo , Komatsu Toru , Abe Hiroyuki , Tsuji Yosuke , Yagi Koichi , Yamagata Yukinori , Aikou Susumu , Nishida Masato , Mori Kazuhiko , Yamashita Hiroharu , Fujishiro Mitsuhiro , Nomura Sachiyo , Shimizu Nobuyuki , Fukayama Masashi , Koike Kazuhiko , Urano Yasuteru , Seto Yasuyuki 
Published: June 2016

AbstractEarly detection of esophageal squamous cell carcinoma (ESCC) is an important prognosticator, but is difficult to achieve by conventional endoscopy. Conventional lugol chromoendoscopy and equipment-based image-enhanced endoscopy, such as narrow-band imaging (NBI), have various practical limitations. Since fluorescence-based visualization is considered a promising approach, we aimed to develop an activatable fluorescence probe to visualize ESCCs. First, based on the fact that various aminopeptidase activities are elevated in cancer, we screened freshly resected specimens from patients with a series of aminopeptidase-activatable fluorescence probes. The results indicated that dipeptidylpeptidase IV (DPP-IV) is specifically activated in ESCCs and would be a suitable molecular target for detection of esophageal cancer. Therefore, we designed, synthesized and characterized a series of DPP-IV-activatable fluorescence probes. When the selected probe was topically sprayed onto endoscopic submucosal dissection (ESD) or surgical specimens, tumors were visualized within 5 min and when the probe was sprayed on biopsy samples, the sensitivity, specificity and accuracy reached 96.9%, 85.7% and 90.5%. We believe that DPP-IV-targeted activatable fluorescence probes are practically translatable as convenient tools for clinical application to enable rapid and accurate diagnosis of early esophageal cancer during endoscopic or surgical procedures.
Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors
Authors: Hedlund Eva , Belnoue Laure , Theofilopoulos Spyridon , Salto Carmen , Bye Chris , Parish Clare , Deng Qiaolin , Kadkhodaei Banafsheh , Ericson Johan , Arenas Ernest , Perlmann Thomas , Simon András 
Published: June 2016

AbstractDegeneration of dopamine neurons in the midbrain causes symptoms of the movement disorder, Parkinson disease. Dopamine neurons are generated from proliferating progenitor cells localized in the embryonic ventral midbrain. However, it remains unclear for how long cells with dopamine progenitor character are retained and if there is any potential for reactivation of such cells after cessation of normal dopamine neurogenesis. We show here that cells expressing Lmx1a and other progenitor markers remain in the midbrain aqueductal zone beyond the major dopamine neurogenic period. These cells express dopamine receptors, are located in regions heavily innervated by midbrain dopamine fibres and their proliferation can be stimulated by antagonizing dopamine receptors, ultimately leading to increased neurogenesis in vivo. Furthermore, treatment with dopamine receptor antagonists enhances neurogenesis in vitro, both from embryonic midbrain progenitors as well as from embryonic stem cells. Altogether our results indicate a potential for reactivation of resident midbrain cells with dopamine progenitor potential beyond the normal period of dopamine neurogenesis.
The study of the relation of DNA repair pathway genes SNPs and the sensitivity to radiotherapy and chemotherapy of NSCLC
Authors: Wang Chunbo , Nie Huan , Li Yiqun , Liu Guiyou , Wang Xu , Xing Shijie , Zhang Liping , Chen Xin , Chen Yue , Li Yu 
Published: June 2016

AbstractTo analyze the relation between SNPs in DNA repair pathway-related genes and sensitivity of tumor radio-chemotherapy, 26 SNPs in 20 DNA repair genes were genotyped on 176 patients of NSCLC undertaking radio-chemotherapy treatment. In squamous cell carcinoma (SCC), as the rs2228000, rs2228001 (XPC), rs2273953 (TP73), rs2279744 (MDM2), rs2299939 (PTEN) and rs8178085, rs12334811 (DNA-PKcs) affected the sensitivity to chemotherapy, so did the rs8178085, rs12334811 to radiotherapy. Moreover rs344781, rs2273953 and rs12334811 were related with the survival time of SCC. In general, the “good” genotype GG (rs12334811) showed greater efficacy of radio-chemotherapy and MSF (24 months) on SCC. In adenocarcinoma, as the rs2699887 (PIK3), rs12334811 (DNA-PKcs) influenced the sensitivity to chemotherapy, so did the rs2299939, rs2735343 (PTEN) to radiotherapy. And rs402710, rs80270, rs2279744 and rs2909430 impacted the survival time of the adenocarcinoma patients. Both GG (rs2279744) and AG (rs2909430) showed a shorter survival time (MFS = 6). Additionally, some SNPs such as rs2228000, rs2228001 and rs344781 were found to regulate the expression of DNA repair pathway genes through eQTLs dataset analysis. These results indicate that SNPs in DNA repair pathway genes might regulate the expression and affect the DNA damage repair, and thereby impact the efficacy of radio-chemotherapy and the survival time of NSCLC.
Shear-induced enhancements of crystallization kinetics and morphological transformation for long chain branched polylactides with different branching degrees
Authors: Wang Junyang , Bai Jing , Zhang Yaqiong , Fang Huagao , Wang Zhigang 
Published: June 2016

AbstractThe effects of long chain branching (LCB) degree on the shear-induced isothermal crystallization kinetics of a series of LCB polylactides (LCB PLAs) have been investigated by using rotational rheometer, polarized optical microscopy (POM) and scanning electron microscopy (SEM). Dynamic viscoelastic properties obtained by small-amplitude oscillatory shear (SAOS) tests indicate that LCB PLAs show more broadened relaxation time spectra with increasing LCB degree. Upon a pre-shear at the shear rate of 1 s−1 LCB PLAs show much faster crystallization kinetics than linear PLA and the crystallization kinetics is enhanced with increasing LCB degree. By modeling the system as a suspension the quantitative evaluation of nucleation density can be derived from rheological experiments. The nucleation density is greatly enhanced with increasing LCB degree and a saturation in shear time is observed. Crystalline morphologies for LCB PLAs observed by POM and SEM demonstrate the enhancement of nucleation density with increasing LCB degree and a transformation from spherulitic to orientated crystalline morphologies. The observation can be ascribed to longer relaxation time of the longest macromolecular chains and broadened, complex relaxation behaviors due to the introduction of LCB into PLA, which is essential in stabilizing the orientated crystal nuclei after pre-shear.
Large area molybdenum disulphide- epitaxial graphene vertical Van der Waals heterostructures
Authors: Pierucci Debora , Henck Hugo , Naylor Carl H. , Sediri Haikel , Lhuillier Emmanuel , Balan Adrian , Rault Julien E. , Dappe Yannick J. , Bertran François , Fèvre Patrick Le , Johnson A. T. Charlie , Ouerghi Abdelkarim 
Published: June 2016

AbstractTwo-dimensional layered transition metal dichalcogenides (TMDCs) show great potential for optoelectronic devices due to their electronic and optical properties. A metal-semiconductor interface, as epitaxial graphene - molybdenum disulfide (MoS2), is of great interest from the standpoint of fundamental science, as it constitutes an outstanding platform to investigate the interlayer interaction in van der Waals heterostructures. Here, we study large area MoS2-graphene-heterostructures formed by direct transfer of chemical-vapor deposited MoS2 layer onto epitaxial graphene/SiC. We show that via a direct transfer, which minimizes interface contamination, we can obtain high quality and homogeneous van der Waals heterostructures. Angle-resolved photoemission spectroscopy (ARPES) measurements combined with Density Functional Theory (DFT) calculations show that the transition from indirect to direct bandgap in monolayer MoS2 is maintained in these heterostructures due to the weak van der Waals interaction with epitaxial graphene. A downshift of the Raman 2D band of the graphene, an up shift of the A1g peak of MoS2 and a significant photoluminescence quenching are observed for both monolayer and bilayer MoS2 as a result of charge transfer from MoS2 to epitaxial graphene under illumination. Our work provides a possible route to modify the thin film TDMCs photoluminescence properties via substrate engineering for future device design.