bluuebunny/crossref_metadata_2025_split huggingface dataset:
2C Proteins of Enteroviruses Suppress IKKβ Phosphorylation by Recruiting Protein Phosphatase 1
Authors: Li Qian , Zheng Zhenhua , Liu Yan , Zhang Zhenfeng , Liu Qingshi , Meng Jin , Ke Xianliang , Hu Qinxue , Wang Hanzhong 
Published: March 2016

ABSTRACT
The NF-κB signaling network, which is an ancient signaling pathway, plays a pivotal role in innate immunity and constitutes a first line of defense against invading pathogens, including viruses. However, numerous viruses possess evolved strategies to antagonize the activation of the NF-κB signaling pathway. Our previous study demonstrated that the nonstructural protein 2C of enterovirus 71 (EV71), which is the major pathogen of hand, foot, and mouth disease, inhibits tumor necrosis factor alpha (TNF-α)-mediated activation of NF-κB by suppressing IκB kinase β (IKKβ) phosphorylation. Nevertheless, the mechanism underlying the inhibition of IKKβ phosphorylation by EV71 2C remains largely elusive. We demonstrate that EV71 2C interacts with all isoforms of the protein phosphatase 1 (PP1) catalytic subunit (the PP1α, PP1β, and PP1γ isoforms) through PP1-docking motifs. EV71 2C has no influence on the subcellular localization of PP1. In addition, the PP1-binding-deficient EV71 2C mutant 3E3L nearly completely lost the ability to suppress IKKβ phosphorylation and NF-κB activation was markedly restored in the mutant, thereby indicating that PP1 binding is efficient for EV71 2C-mediated inhibition of IKKβ phosphorylation and NF-κB activation. We further demonstrate that 2C forms a complex with PP1 and IKKβ to dephosphorylate IKKβ. Notably, we reveal that other human enteroviruses, including poliovirus (PV), coxsackie A virus 16 (CVA16), and coxsackie B virus 3 (CVB3), use 2C proteins to recruit PP1, leading to the inhibition of IKKβ phosphorylation. Our findings indicate that enteroviruses exploit a novel mechanism to inhibit IKKβ phosphorylation by recruiting PP1 and IKKβ to form a complex through 2C proteins, which ultimately results in the inhibition of the NF-κB signaling pathway.

IMPORTANCE
The innate antiviral immunity system performs an essential function in recognizing and eliminating invading viruses. Enteroviruses include a number of important human pathogens, including poliovirus (PV), EV71, and coxsackieviruses (CVs). As 2C is the most conserved and complex nonstructural protein of enteroviruses, its biological function is largely unclear, whereas the 2A and 3C proteinases of enteroviruses are well characterized. We reveal that EV71 2C forms a complex with PP1 and IKKβ to maintain IKKβ in an unphosphorylated and inactive state, resulting in the inactivation of the TNF-α-mediated NF-κB signaling pathway. We provide evidence that the 2C proteins of the enteroviruses PV, CVA16, and CVB3 suppress IKKβ phosphorylation through the same mechanism involving PP1. We demonstrate that enteroviruses exploit a novel mechanism involving PP1 to regulate innate antiviral immunity, and our findings may be particularly important for understanding the pathogenicity of enteroviruses.
The prevalence of hyperglycaemia and its relationship with mortality, readmissions and length of stay in an older acute surgical population: a multicentre study
Authors: Myint Phyo Kyaw , Owen Stephanie , Pearce Lyndsay , Greig Matthew F , Tay Hui Sian , McCormack Caroline , McCarthy Kathryn , Moug Susan J , Stechman Michael J , Hewitt Jonathan 
Published: March 2016

AbstractBackgroundThe purpose of the study is to examine the prevalence of hyperglycaemia in an older acute surgical population and its effect on clinically relevant outcomes in this setting.MethodsUsing Older Persons Surgical Outcomes Collaboration (OPSOC) multicentre audit data 2014, we examined the prevalence of admission hyperglycaemia, and its effect on 30-day and 90-day mortality, readmission within 30 days and length of acute hospital stay using logistic regression models in consecutive patients, ≥65 years, admitted to five acute surgical units in the UK hospitals in England, Scotland and Wales. Patients were categorised in three groups based on their admission random blood glucose: &lt;7.1, between 7.1 and 11.1 and ≥11.1 mmol/L.ResultsA total of 411 patients (77.25±8.14 years) admitted during May and June 2014 were studied. Only 293 patients (71.3%) had glucose levels recorded on admission. The number (%) of patients with a blood glucose &lt;7.1, 7.1–11.1 and ≥11.1 mmol/L were 171 (58.4), 99 (33.8) and 23 (7.8), respectively. On univariate analysis, admission hyperglycaemia was not predictive of any of the outcomes investigated. Although the characteristics of those with no glucose level were not different from the included sample, 30-day mortality was significantly higher in those who had not had their admission glucose level checked (10.2% vs 2.7%), suggesting a potential type II error.ConclusionDespite current guidelines, nearly a third of older people with surgical diagnoses did not have their glucose checked on admission highlighting the challenges in prognostication and evaluation research to improve care of older frail surgical patients.
The missing piece of the South Atlantic jigsaw: when continental break-up ignores crustal heterogeneity
Authors: Paton D. A. , Mortimer E. J. , Hodgson N. , van der Spuy D. 
Published: March 2016

Abstract
Crustal heterogeneity is considered to play a critical role in the position of continental break-up, yet this can only be demonstrated when a fully constrained pre-break-up configuration of both conjugate margins is achievable. Limitations in our understanding of the pre-break-up crustal structure in the offshore region of many margins preclude this. In the southern South Atlantic, which is an archetypal conjugate margin, this can be achieved because of the high confidence in plate reconstruction. Prior to addressing the role of crustal heterogeneity, two questions have to be addressed: first, what is the location of the regionally extensive Gondwanan Orogeny that remains enigmatic in the Orange Basin, offshore South Africa; and, second, although it has been established that the Argentinian Colorado rift basin has an east–west trend perpendicular to the Orange Basin and Atlantic spreading, where is the western continuation of this east–west trend? We present here a revised structural model for the southern South Atlantic by identifying the South African fold belt offshore. The fold belt trend changes from north–south to east–west offshore and correlates directly with the restored Colorado Basin. The Colorado–Orange rifts form a tripartite system with the Namibian Gariep Belt, which we call the Garies Triple Junction. All three rift branches were active during the break-up of Gondwana, but during the Atlantic rift phase the Colorado Basin failed while the other two branches continued to rift, defining the present day location of the South Atlantic. In addressing these two outstanding questions, this study challenges the premise that crustal heterogeneity controls the position of continental break-up because seafloor spreading demonstrably cross-cuts the pre-existing crustal heterogeneity. Furthermore, we highlight the importance of differentiating between early rift evolution and subsequent rifting that occurs immediately prior to seafloor spreading.
PD-L1 Negative Status is Associated with Lower Mutation Burden, Differential Expression of Immune-Related Genes, and Worse Survival in Stage III Melanoma
Authors: Madore Jason , Strbenac Dario , Vilain Ricardo , Menzies Alexander M. , Yang Jeen Y. H. , Thompson John F. , Long Georgina V. , Mann Graham J. , Scolyer Richard A. , Wilmott James S. 
Published: March 2016

Abstract
Purpose: Understanding why some melanomas test negative for PD-L1 by IHC may have implications for the application of anti-PD-1 therapies in melanoma management. This study sought to determine somatic mutation and gene expression patterns associated with tumor cell PD-L1 expression, or lack thereof, in stage III metastatic melanoma to better define therapeutically relevant patient subgroups.
Experimental Design: IHC for PD-L1 was assessed in 52 American Joint Committee on Cancer stage III melanoma lymph node specimens and compared with specimen-matched comprehensive clinicopathologic, genomic, and transcriptomic data.
Results: PD-L1–negative status was associated with lower nonsynonymous mutation (NSM) burden (P = 0.017) and worse melanoma-specific survival [HR = 0.28 (0.12–0.66), P = 0.002] in stage III melanoma. Gene set enrichment analysis identified an immune-related gene expression signature in PD-L1–positive tumors. There was a marked increase in cytotoxic T-cell and macrophage-specific genes in PD-L1–positive melanomas. CD8Ahigh gene expression was associated with better melanoma-specific survival [HR = 0.2 (0.05–0.87), P = 0.017] and restricted to PD-L1–positive stage III specimens. NF1 mutations were restricted to PD-L1–positive tumors (P = 0.041).
Conclusions: Tumor negative PD-L1 status in stage III melanoma lymph node metastasis is a marker of worse patient survival and is associated with a poor immune response gene signature. Lower NSM levels were associated with PD-L1–negative status suggesting differences in somatic mutation profiles are a determinant of PD-L1–associated antitumor immunity in stage III melanoma. Clin Cancer Res; 22(15); 3915–23. ©2016 AACR.
Three new species of Sectonema Thorne, 1930 (Dorylaimida: Aporcelaimidae) from Vietnam
Authors: Álvarez-Ortega Sergio , Nguyen Thi Anh Duong , Abolafia Joaquín , Vu Thi Thanh Tam , Bonkowski Michael , Peña-Santiago Reyes 
Published: March 2016

Three new species of the genus Sectonema collected from natural habitats in Vietnam are studied, described and illustrated, including line drawings, LM and/or SEM pictures. Sectonema birrucephalum sp. n. is characterised by its 2.73-4.35 mm long body, lip region 18-20 μm broad and offset by deep constriction, odontostyle 10.0-11.5 μm long on its ventral side, 659-989 μm long neck, pharyngeal expansion occupying 63-68% of total neck length, uterus a simple tube, 221-277 μm long, pars refringens vaginae present, V = 54-56, tail short (31-43 μm, c = 85-111, c′ = 0.6-0.8) and rounded, spicules 72-75 μm long, and four or five irregularly spaced ventromedian supplements beyond the range of the spicules. Sectonema buccociliatum sp. n. is distinguished by its 2.00-2.46 mm long body, lip region offset by constriction, 19-20 μm broad and bearing perioral cilia-like structures, odontostyle 13-14 μm long at its ventral side, 530-625 μm long neck, pharyngeal expansion occupying 62-69% of total neck length, uterus a simple tube, 116-152 μm long, pars refringens vaginae present, V = 56-62, tail short (23-31 μm, c = 72-104, c′ = 0.6-0.8) and rounded, spicules 56-68 μm long, and 3-5 spaced and weakly developed ventromedian supplements beyond the range of the spicules. Sectonema ciliatum sp. n. is characterised by its 2.79-3.13 mm long body, lip region offset by constriction, 21-22 μm broad and bearing perioral cilia-like structures, odontostyle 14-15 μm long at its ventral side, 699-722 μm long neck, pharyngeal expansion occupying 60% of total neck length, uterus a simple tube, 201-244 μm long, pars refringens vaginae present, V = 52-53, tail short (33-35 μm, c = 82-92, c′ = 0.6-0.7) and rounded, spicules 70-72 μm long, and three or four spaced and weakly developed ventromedian supplements beyond the range of the spicules. Molecular data obtained for S. ciliatum sp. n. and the derived evolutionary tree show a close phylogenetic relationship with other species of the genus.
A genus-level classification of the family Thraupidae (Class Aves: Order Passeriformes)
Authors: BURNS KEVIN J. , UNITT PHILIP , MASON NICHOLAS A. 
Published: March 2016

The tanagers (Thraupidae) are a major component of the Neotropical avifauna, and vary in plumage colors, behaviors, morphologies, and ecologies. Globally, they represent nearly 4% of all avian species and are the largest family of songbirds. However, many currently used tanager genera are not monophyletic, based on analyses of molecular data that have accumulated over the past 25 years. Current genus-level classifications of tanagers have not been revised according to newly documented relationships of tanagers for various reasons: 1) the lack of a comprehensive phylogeny, 2) reluctance to lump existing genera into larger groups, and 3) the lack of available names for newly defined smaller groups. Here, we present two alternative classifications based on a newly published comprehensive phylogeny of tanagers. One of these classifications uses existing generic names, but defines them broadly. The other, which we advocate and follow here, provides new generic names for more narrowly defined groups. Under the latter, we propose eleven new genera (Asemospiza, Islerothraupis, Maschalethraupis, Chrysocorypha, Kleinothraupis, Castanozoster, Ephippiospingus, Chionodacryon, Pseudosaltator, Poecilostreptus, Stilpnia), and resurrect several generic names to form monophyletic taxa. Either of these classifications would allow taxonomic authorities to reconcile classification with current understanding of tanager phylogenetic relationships. Having a more phylogenetically accurate classification for tanagers will facilitate the study and conservation of this important Neotropical radiation of songbirds.
Dynamics of the Block Island Sound Estuarine Plume
Authors: Liu Qianqian , Rothstein Lewis M. , Luo Yiyong 
Published: March 2016

AbstractBuoyant discharge of freshwater from Long Island Sound (LIS) forms a seasonal buoyant plume outside Block Island Sound (BIS) between the coast of Long Island and the denser shelf waters. The plume’s seasonal variability and its response to tides, winds, and surface heating are investigated through a series of process-oriented experiments using the Regional Ocean Modeling System (ROMS). Results show the importance of river discharge, wind directions, and surface heating in the seasonal variation of the BIS buoyant plume. In winter and spring, the plume is intermediate with a large surface offshore extension detached from the bottom. From winter to spring, the river discharge increases; meanwhile, upwelling-favorable winds keep dominating. They compete with the increase of surface heating and generate a broader buoyant plume in spring than in winter. In summer, the plume is bottom advected with most of its width in contact with the bottom and is featured with the steepest isopycnals and narrowest plume, which is driven by a combination of strong insolation, weak buoyant discharge from LIS, and feeble winds. In fall, although the river discharge is comparable to that in winter, the upwelling-favorable wind is relatively weaker, corresponding to a narrower intermediate plume.
Observations of the Transfer of Energy and Momentum to the Oceanic Surface Boundary Layer beneath Breaking Waves
Authors: Scully Malcolm E. , Trowbridge John H. , Fisher Alexander W. 
Published: March 2016

AbstractMeasurements just beneath the ocean surface demonstrate that the primary mechanism by which energy from breaking waves is transmitted into the water column is through the work done by the covariance of turbulent pressure and velocity fluctuations. The convergence in the vertical transport of turbulent kinetic energy (TKE) balances the dissipation rate of TKE at first order and is nearly an order of magnitude greater than the sum of the integrated Eulerian and Stokes shear production. The measured TKE transport is consistent with a simple conceptual model that assumes roughly half of the surface flux of TKE by wave breaking is transmitted to depths greater than the significant wave height. During conditions when breaking waves are inferred, the direction of momentum flux is more aligned with the direction of wave propagation than with the wind direction. Both the energy and momentum fluxes occur at frequencies much lower than the wave band, consistent with the time scales associated with wave breaking. The largest instantaneous values of momentum flux are associated with strong downward vertical velocity perturbations, in contrast to the pressure work, which is associated with strong drops in pressure and upward vertical velocity perturbations.
Recovery, non-profit organisations and mental health services: ‘Hit and miss’ or ‘dump and run’?
Authors: Hungerford Catherine , Hungerford Alice , Fox Cathy , Cleary Michelle 
Published: March 2016

Background: The evolution of Recovery-oriented mental health services in Western nations across the globe has given rise to a growth in community-based psychosocial support services, to assist in meeting the diverse needs of consumers. This article reports findings of research that explored the perceptions of community workers who are employed by non-profit organisations and deliver psychosocial support services to support delivery of Recovery-oriented clinical mental health services. Aims: The focus of the research reported in this article includes the benefits and challenges encountered by the community workers when working with clinicians. Method: The research was undertaken as part of a single-case embedded study, which evaluated the implementation of Recovery-oriented approaches to the delivery of clinical mental health services in a major urban centre located in south-eastern Australia. Results: Generally, community workers employed by the non-profit organisations perceived the implementation of Recovery-oriented clinical mental health services to be a positive step forward for consumers. Challenges to the delivery of Recovery-oriented services included issues arising from the many different understandings of what it means to experience mental health Recovery, the quality of communication between the community workers and clinicians and the clinicians’ lack of understanding of the role of non-profit organisations and community workers. Conclusion: The article concludes with recommendations to address the challenges involved, with a view to improving the partnerships between community workers and clinicians, and the Recovery journey of people with serious mental illness.
Sevoflurane anesthesia during acute right ventricular ischemia in pigs preserves cardiac function better than propofol anesthesia
Authors: Haraldsen Pernille , Metzsch Carsten , Lindstedt Sandra , Algotsson Lars , Ingemansson Richard 
Published: March 2016

Background: The intention of the present study was to evaluate possible cardioprotective properties of inhalation anesthesia with sevoflurane. Methods and Materials: A porcine, open-chest model of right ventricular ischemia was used in 7 pigs receiving inhalation anesthesia with sevoflurane. The model was earlier developed and published by our group, using pigs receiving intravenous anesthesia with propofol. They served as controls. The animals were observed for three hours after the induction of right ventricular ischemia by ligation of the main branches supplying the right ventricular free wall. Results: In the sevoflurane group, the cardiac output recovered 2 hours after the induction of ischemia and intact right ventricular stroke work was observed. In the propofol group, no such recovery occurred. The release of troponin T was significantly lower than in the sevoflurane group. Conclusions: Inhalation anesthesia with sevoflurane seems superior to intravenous anesthesia with propofol in acute right ventricular ischemic dysfunction.
End-of-life care preferences of nursing home residents: Results of a cross-sectional study
Authors: Ng Charis Wei Ling , Cheong SK , Govinda Raj A , Teo WSK , Leong IYO 
Published: March 2016

Background: Palliative care services were not available in nursing homes in Singapore. Project CARE (Care At the end-of-life for Residents in homes for the Elderly) was a pilot programme that aimed to promote advance care planning and improve end-of-life care in nursing homes. Aim: We aimed to examine end-of-life care preferences among nursing home residents, and identify factors associated with preference for medical intervention, cardiopulmonary resuscitation and place of death. Design and setting/participants: A cross-sectional study using data from advance care planning discussions was conducted from September 2009 to April 2012 across seven nursing homes. The advance care planning discussion was conducted with the resident (with a prognosis of 6 months or 1 year), their families and staff from the nursing home and hospital. Results: A total of 600 residents and their families completed the advance care planning discussion. Majority (93.2%) preferred not to proceed with cardiopulmonary resuscitation, 52.3% opted for limited additional intervention at the nursing home with escalation to the hospital if necessary and 77.0% preferred to die at the nursing home. Residents 85+ years (relative risk ratio: 3.34, 95% confidence interval: 1.13–9.93, p = 0.030) were more likely to prefer medical intervention at the nursing home only. No associations were found with the preference for cardiopulmonary resuscitation. Residents who were single, or who were Christians or Catholics (adjusted odds ratio: 2.09, 95% confidence interval: 1.04–4.19, p = 0.039), were more likely to prefer to die at the nursing home. Conclusion: Preferences for medical interventions in nursing homes provide support to extend palliative care services to nursing homes, which may benefit residents who are older, single, or Christians or Catholics.