bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of corona discharge plasma jet on surface‐borne microorganisms and sprouting of broccoli seeds
Authors: Kim Je‐Wook , Puligundla Pradeep , Mok Chulkyoon 
Published: March 2016

AbstractBACKGROUNDDifferent pathogenic microorganisms have been reported to cause sprouts‐associated outbreaks. In order to sterilise and enhance the germination of seeds, non‐thermal plasma has been increasingly investigated in the field of agricultural science as an alternative to the traditional pre‐sowing seed treatments. This work aimed to evaluate the effect of corona discharge plasma jet (CDPJ) on disinfection of the natural bio‐contaminants of broccoli seed and also studied the plasma effect on sprout seed germination rate and physico‐chemical properties of sprouts.RESULTSAerobic bacteria, moulds and yeasts, B. cereus, E. coli, Salmonella spp. were detected on the broccoli seed surface. After 0–3 min treatment using CDPJ, the detected microorganisms were reduced in the range of 1.2–2.3 log units. Inactivation patterns were better explained using pseudo‐first‐order kinetics. The plasma treatment of seeds up to 2 min exhibited a positive effect on germination rate, seedling growth. The physico‐chemical and sensory characteristics of sprouts were unaffected due to the CDPJ treatment of their respective seeds.CONCLUSIONCorona discharge plasma jet can potentially be used for microbial decontamination of broccoli seeds. In addition, the plasma treatment of broccoli sprout seeds has enabled a significant enhancement in their germination rate and seedling growth without compromising physico‐chemical and sensory characteristics of their corresponding sprouts. © 2016 Society of Chemical Industry
Azacitidine‐Induced Pericarditis: A Case Series
Authors: Newman Matthew , Malla Midhun , Gojo Ivana 
Published: March 2016

Study ObjectiveTo describe three cases of pericarditis probably related to azacitidine administration in a span of 3 years at our center.DesignCase series.SettingComprehensive cancer center within a large, academic medical center.PatientsThree patients with high‐grade myelodysplastic syndrome or acute myeloid leukemia who received azacitidine.InterventionNone.MeasurementsNone.Main ResultsPatient 1 presented with pericarditis after cycle 2 of azacitidine, patient 3 presented 3 weeks after completing cycle 5, and patient 2 presented during cycle 1. All patients were treated symptomatically and responded to corticosteroids. None of the patients were re‐challenged with hypomethylating agents. Use of the Naranjo adverse drug reaction probability scale indicated a probable adverse drug reaction (score of 6) for patients 1 and 3 and a possible adverse drug reaction (score of 3) for patient 2.ConclusionWith the exclusion of other common causes of pericarditis, we believe it is likely that azacitidine was responsible for the findings in our patients. Providers caring for patients receiving hypomethylating agents should consider this potential adverse drug reaction in the setting of unexplained chest pain or other clinical signs consistent with cardiotoxicity.
Dynamic behavior of the post‐SET loop region of NSD1: Implications for histone binding and drug development
Authors: Graham Sarah E. , Tweedy Sara E. , Carlson Heather A. 
Published: March 2016

AbstractNSD1 is a SET‐domain histone methyltransferase that methylates lysine 36 of histone 3. In the crystal structure of NSD1, the post‐SET loop is in an autoinhibitory position that blocks binding of the histone peptide as well as the entrance to the lysine‐binding channel. The conformational dynamics preceding histone binding and the mechanism by which the post‐SET loop moves to accommodate the target lysine is currently unknown, although potential models have been proposed. Using molecular dynamics simulations, we have identified potential conformations of the post‐SET loop differing from those of previous studies, as well as proposed a model of peptide‐bound NSD1. Our simulations illustrate the dynamic behavior of the post‐SET loop and the presence of a few distinct conformations. In every case, the post‐SET loop remains in an autoinhibitory position blocking the peptide‐binding cleft, suggesting that another interaction is required to optimally position NSD1 in an active conformation. This finding provides initial evidence for a mechanism by which NSD1 preferentially binds nucleosomal substrates.
Three reasons protein disorder analysis makes more sense in the light of collagen
Authors: Smithers Ben , Oates Matt E. , Tompa Peter , Gough Julian 
Published: March 2016

AbstractWe have identified that the collagen helix has the potential to be disruptive to analyses of intrinsically disordered proteins. The collagen helix is an extended fibrous structure that is both promiscuous and repetitive. Whilst its sequence is predicted to be disordered, this type of protein structure is not typically considered as intrinsic disorder. Here, we show that collagen‐encoding proteins skew the distribution of exon lengths in genes. We find that previous results, demonstrating that exons encoding disordered regions are more likely to be symmetric, are due to the abundance of the collagen helix. Other related results, showing increased levels of alternative splicing in disorder‐encoding exons, still hold after considering collagen‐containing proteins. Aside from analyses of exons, we find that the set of proteins that contain collagen significantly alters the amino acid composition of regions predicted as disordered. We conclude that research in this area should be conducted in the light of the collagen helix.
Silica Microparticles Supported Gold and Copper Ferrite Nanoparticles: A Magnetically Recyclable Bimetallic Catalyst for Sonogashira Reaction
Authors: Gholinejad Mohammad , Ahmadi Jahantab , Nájera Carmen 
Published: March 2016

AbstractNovel silica supported gold and copper ferrite nanoparticles (NPs) have been synthesized, characterized and used as a separable dual catalyst in Sonogashira type reaction. These Au.CuFe2O4@Silica NPs show a high efficiency as catalyst in the alkynylation not only of aryl iodides but also aryl bromides. By using only 0.5 mol% loading and t‐BuOK as base in N,N‐dimethylacetamide as solvent, aryl iodides react at 115 ºC in 1 d, whereas for aryl bromides the cross‐coupling takes place at 130 ºC in 2 d. The catalyst can be successfully recycled using an external magnet for four consecutive runs.
Triazine‐Triazole Conjugates as Potent Ligands for Cu–Catalyzed Sonogashira Reaction
Authors: Wang Zhenhua , Wang Xiu , Sun Huaming , Zhu Zhuangli , Zhang Guofang , Zhang Weiqiang , Gao Ziwei 
Published: March 2016

AbstractTriazine Triazole conjugates (TT) were developed as efficient ligand system for CuSO4⋅5H2O catalyzed Sonogashira and decarboxylative cross‐coupling reaction. This new protocol showed good compatibility with a broad range of substitutents and afforded moderate to excellent yields. TT ligands are rapid constructed and highly modular electronically and sterically.
Schneiderian first rank symptoms in schizophrenia: A developmental neuroscience evaluation
Authors: Tikka Sai Krishna , Nizamie S.Haque , Das Archana Kumari , Agarwal Nidhi , Goyal Nishant 
Published: March 2016

AbstractIntroductionSelf disorders in schizophrenia have been suggested to have distinct neurobiological underpinnings. Using comprehensive neuro‐scientific assessments including a neurophysiological, a neurochemical and a neuropsychological marker, this study assesses disordered‐“self” in schizophrenia.MethodsTwenty schizophrenia patients with first rank symptoms (FRS;FRS+), 20 patients without FRS (FRS−) and 20 healthy controls (HC) were assessed for psychopathology, especially on specially designed FRS score sheets with a narrow and a broad definition. Resting state electroencephalography was acquired using 256‐electrodes; gamma spectral‐power was measured in 8 regions of interest. Serum BDNF and self‐monitoring were also assessed. Comparative and correlation analysis were conducted in addition to a step‐wise discriminant function analysis.ResultsFRS+ group with greater positive symptom score and a lower negative symptom score, showed significantly increased gamma spectral power, especially on right hemispheric regions, along with lower BDNF levels and lower scores on self‐monitoring compared to FRS− and HC. Serum BDNF levels and gamma spectral power in the region corresponding right inferior parietal lobule were identified as predictors that most accurately classified the defined groups.ConclusionsSchizophrenia patients satisfying the criteria of presence of first rank symptoms represent a distinct neurodevelopmental subgroup with associated features of predominantly positive symptoms, significantly lower neurotrophin levels, aberrant resting state brain activity in the heteromodal association cortex and performing poorer on self‐monitoring tasks.
Genetic susceptibility to postherniotomy pain. The influence of polymorphisms in the Mu opioid receptor, TNF-α, GRIK3, GCH1, BDNF and CACNA2D2 genes
Authors: Kalliomäki Maija-Liisa , Sandblom Gabriel , Hallberg Mathias , Grönbladh Alfhild , Gunnarsson Ulf , Gordh Torsten , Ginya Harumi , Nyberg Fred 
Published: March 2016

Abstract

Background and aims
Despite improvements in surgical technique, 5%-8% of patients undergoing herniorrhaphy still suffer from clinically relevant persistent postherniotomy pain. This is a problem at both individual and society levels. The aim of this study was to determine whether or not a single nucleotide polymorphism in a specific gene contributes to the development of persistent pain after surgery.

Methods
One hundred individuals with persistent postherniotomy pain, along with 100 without pain matched for age, gender and type of surgery were identified in a previous cohort study on patients operated for groin hernia. All patients underwent a thorough sensory examination and blood samples were collected. DNA was extracted and analysed for single nucleotide polymorphism in the Mu opioid receptor, TNF-α, GRIK3, GCH1, BDNF and CACNA2D2 genes.

Results
Patients with neuropathic pain were found to have a homozygous single nucleotide polymorph in the TNF-α gene significantly more often than pain-free patients (P =0.036, one-tailed test).

Conclusions
SNP in the TNF-α gene has a significant impact on the risk for developing PPSP.

Implications
The result suggests the involvement of genetic variance in the development of pain and this requires further investigation.
Layered hydrogels accelerate iPSC-derived neuronal maturation and reveal migration defects caused by MeCP2 dysfunction
Authors: Zhang Zhen-Ning , Freitas Beatriz C. , Qian Hao , Lux Jacques , Acab Allan , Trujillo Cleber A. , Herai Roberto H. , Nguyen Huu Viet Anh , Wen Jessica H. , Joshi-Barr Shivanjali , Karpiak Jerome V. , Engler Adam J. , Fu Xiang-Dong , Muotri Alysson R. , Almutairi Adah 
Published: March 2016

Significance
Three-dimensional systems enable the formation of tissue-mimetic architectures and promote more realistic physiological responses than conventional 2D systems. Here we report a previously unidentified layered 3D culture system to assay migration and maturation of human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs) and reveal a genotype-specific effect of methyl-CpG-binding protein-2 (MeCP2) dysfunction on iPSC-derived neuronal migration and maturation in 3D layered hydrogels. Using this platform, we identified a migration defect in MeCP2-mutant iPSC-derived NPCs and confirmed previous observations that neurons derived from these cells have reduced neurite outgrowth and fewer synapses. Meanwhile, 3D hydrogel culture accelerates neuronal differentiation of iPSC-derived NPCs.
Tudor Humanists, London Printers, and the Status of Women: The Struggle over Livy in theQuerelle des Femmes
Authors: Philo John-Mark 
Published: March 2016

AbstractThis essay discusses a novel contribution to the Renaissance debate over women. In 1551, William Thomas translated a brief but significant moment from Livy’sHistory of Romeconcerning the repeal of the Lex Oppia, a sumptuary law targeting women in particular. Thomas thereby adapted one of the most arresting examples of women’s engagement in Roman politics. The episode shows the women of Rome taking to the streets to demand the law’s repeal, forcing senators and tribunes alike to acknowledge their protest. By contextualizing Thomas’s translation amid Quattrocento debates over female apparel and contemporary, female-centric works printed by Thomas Berthelet, Thomas’s translation emerges as a clear though hitherto-unacknowledged intervention in the Englishquerelle des femmes.
The Hunt in the Performance of Archducal Rule: Endurance and Revival in the Habsburg Netherlands in the Early Seventeenth Century
Authors: Duerloo Luc 
Published: March 2016

AbstractAfter decades of civil war, the archdukes Albert and Isabella drew on the princely hunt to consolidate their regime in the Habsburg Netherlands. In essence a performance of dominion, the hunt served to enact their exalted birth and the return to peace and order. Attempts to unify the hunting laws of their possessions were, however, frustrated by provincial liberties. As a result the archducal hunt had to derive its prestige from where it was held, rather than from the species that were hunted. Appropriating the cult of Saint Hubertus and patronizing artistic representations further enhanced the prestige of these locations.
Fictions of Abundance in Early Modern Madrid: Hospitality, Consumption, and Artistic Identity in the Work of Juan van der Hamen y León
Authors: Ripollés Carmen 
Published: March 2016

AbstractThis article examines how still-life painting contributed to the creation of a distinct urban aristocratic culture in seventeenth-century Madrid. Focusing on a group of paintings by Juan van der Hamen, the article situates these images within the context of the picture gallery and the practice of aristocratic hospitality. By giving visual form to this new urban mode of magnificence, Van der Hamen’s still lifes created a fiction of abundance that glossed over Madrid’s economic realities. At the same time, Van der Hamen concealed signs of manual craftsmanship and commercial interest in order to advance and ennoble his own artistic identity.
Intraoperative Secondary Insults During Orthopedic Surgery in Traumatic Brain Injury
Authors: Algarra Nelson N. , Lele Abhijit V. , Prathep Sumidtra , Souter Michael J. , Vavilala Monica S. , Qiu Qian , Sharma Deepak 
Published: March 2016

Background:
Secondary insults worsen outcomes after traumatic brain injury (TBI). However, data on intraoperative secondary insults are sparse. The primary aim of this study was to examine the prevalence of intraoperative secondary insults during orthopedic surgery after moderate-severe TBI. We also examined the impact of intraoperative secondary insults on postoperative head computed tomographic scan, intracranial pressure (ICP), and escalation of care within 24 hours of surgery.

Materials and Methods:
We reviewed medical records of TBI patients 18 years and above with Glasgow Coma Scale score <13 who underwent single orthopedic surgery within 2 weeks of TBI. Secondary insults examined were: systemic hypotension (systolic blood pressure<90 mm Hg), intracranial hypertension (ICP>20 mm Hg), cerebral hypotension (cerebral perfusion pressure<50 mm Hg), hypercarbia (end-tidal CO2>40 mm Hg), hypocarbia (end-tidal CO2<30 mm Hg in absence of intracranial hypertension), hyperglycemia (glucose>200 mg/dL), hypoglycemia (glucose<60 mg/dL), and hyperthermia (temperature >38°C).

Results:
A total of 78 patients (41 [18 to 81] y, 68% male) met the inclusion criteria. The most common intraoperative secondary insults were systemic hypotension (60%), intracranial hypertension and cerebral hypotension (50% and 45%, respectively, in patients with ICP monitoring), hypercarbia (32%), and hypocarbia (29%). Intraoperative secondary insults were associated with worsening of head computed tomography, postoperative decrease of Glasgow Coma Scale score by ≥2, and escalation of care. After Bonferroni correction, association between cerebral hypotension and postoperative escalation of care remained significant (P<0.001).

Conclusions:
Intraoperative secondary insults were common during orthopedic surgery in patients with TBI and were associated with postoperative escalation of care. Strategies to minimize intraoperative secondary insults are needed.
Factors in Surgical Decision Making for Thoracolumbar/Lumbar AIS: It’s About More Than Just the Curve Magnitude
Authors: Souder Christopher , Newton Peter O. , Shah Suken A. , Lonner Baron S. , Bastrom Tracey P. , Yaszay Burt 
Published: March 2016

Study Design:
A retrospective review of prospective data

Objective:
The purpose of this study was to compare operative and nonoperative patients with similar curve magnitudes to determine motivating factors associated with surgical correction in “smaller” curves.

Summary of Background Data:
Despite traditional treatment recommendations on major curve angle measurements, many patients with thoracolumbar/lumbar (TH/L) curves of smaller magnitudes are unhappy and desire correction.

Methods:
A prospectively enrolled multicenter adolescent idiopathic scoliosis database was queried. Patients with major TH/L curves <50 degrees and low risk of progression (Risser 3, 4, and 5) were identified and grouped based on their treatment (operative vs. nonoperative). Preoperative demographic, radiographic, Scoliosis Research Society (SRS) outcome scores, and trunk shape values were compared.

Results:
A total of 126 patients undergoing surgical intervention and 17 patients pursuing nonoperative treatment were analyzed. The average lumbar curve of the operative group was 43 degrees (range, 35 to 49 degrees) and for the nonoperative group was 39 degrees (range, 26 to 49 degrees). The operative group was significantly younger, had larger lumbar major curve angles, lower thoracic to lumbar curve ratio, increased TH/L apical translation, and greater trunk shift (P<0.05). Only lumbar curve (P=0.018, OR=1.19) and trunk shift (P=0.01, OR=3.22) remained significant predictors of surgery in a multivariate regression analysis. SRS scores were significantly lower in the operative group for pain, self-image, function, mental health, and total (P<0.05). When SRS total score was entered into the regression, it was the only significant predictor of surgical intervention (P=0.004, OR=0.03).

Conclusions:
Many patients with smaller lumbar curves have clinical deformities that are more consistent with larger curves. These smaller curves can produce similar coronal imbalance and trunk shift, with lower SRS domains that may drive patients to seek surgical treatment. What is unclear is the reason for this greater degree of imbalance in this select group of patients and whether the natural history is different for a more balanced TH/L curve.

Level of Evidence:
Level II—Prognostic.
Early-stage Uterine Pure and Mixed Clear Cell Carcinoma
Authors: Chang-Halpenny Christine N. , Natarajan Sathima , Hwang-Graziano Julie M. 
Published: March 2016

Objective:
Clear cell carcinoma (CCC) of the uterus is a rare but aggressive histology for which the role of adjuvant therapy for stage I-II disease is unclear. Our study investigated outcomes and patterns of failure in these patients.

Methods:
We found 64 cases of CCC, including 26 of pure CCC, 22 mixed with endometrioid adenocarcinoma, and 16 mixed with uterine papillary serous carcinoma. Adjuvant treatment was given to 55%.

Results:
Median follow-up was 51.9 months. By Kaplan-Meier estimate, 5-year vaginal recurrence-free survival (RFS) was 91.3%, pelvic RFS was 92.6%, distant metastasis RFS was 81.6%, disease-free survival was 79.6%, and overall survival was 79.7%. Median time to recurrence was 20.7 months (range, 2 to 40.5 mo). Patients treated adjuvantly had higher proportion of stage II disease (40% vs. 6.9% observed, P=0.0031) and 20% (7/35) recurred. There were no significant differences in outcomes by histologic subtypes but numerically more recurrences with uterine papillary serous involvement. By univariate analysis, higher stage, presence of lymphovascular invasion, and lack of lymph node dissection were predictive of worse overall survival. Age 65 years and above was predictive of worse cancer-specific survival. Of 12 who progressed, only 1 was salvaged and 11 died of disease. Of progressors, 10 had documented distant metastasis. Median time from recurrence to death was 4.5 months (range, 0.2 to 21.2 mo).

Conclusions:
Given aggressive and often unsalvageable nature of recurrence, consideration of adjuvant treatment (including chemotherapy and radiation) is warranted for early-stage CCC, particularly for stage II or those with poor prognostic factors.
Recombinant Human Alpha-1 Antitrypsin-Fc Fusion Protein Reduces Mouse Myocardial Inflammatory Injury After Ischemia–Reperfusion Independent of Elastase Inhibition
Authors: Toldo Stefano , Mauro Adolfo G. , Marchetti Carlo , Rose Scott W. , Mezzaroma Eleonora , Van Tassell Benjamin W. , Kim Soohyun , Dinarello Charles A. , Abbate Antonio 
Published: March 2016

Background:
Alpha-1-antitrypsin (AAT) is an abundant plasma protein with neutrophil elastase-inhibiting activity, and AAT is available as a plasma-derived therapeutic (pAAT). In experimental myocardial infarction, pAAT reduced acute inflammatory injury because of ischemia–reperfusion. The aim of the present study was to assess the properties of a recombinant protein composed of human AAT fused to the human immunoglobulin (Ig) G1 Fc fragment (rhAAT-Fc) in experimental myocardial infarction.

Methods:
Ten-week-old CD1 male mice underwent transient occlusion (30 minutes) of the left anterior coronary artery. rhAAT-Fc (2 mg/kg) or pAAT (60 mg/kg) were administered upon reperfusion. We used human plasma-derived Ig (2 mg/kg) or a matching volume of NaCl 0.9% as control solutions. After 24 hours, infarct size and caspase-1 activity were quantified. The left ventricular ejection fraction (LVEF) was measured by echocardiography at 24 hours and 7 days. A variant of rhAAT-Fc lacking elastase inhibition activity, rhAAT-Fc[E−], was also tested.

Results:
The rhAAT-Fc induced a significant reduction in infarct size (P < 0.01 vs. all controls, P > 0.05 vs. pAAT). Caspase-1 activity was reduced to the same degree with rhAAT-Fc and pAAT (−70%; P < 0.05; P > 0.05 rhAAT-Fc vs. pAAT). The effects on infarct size after a single administration were reflected by preservation of LVEF at 24 hours and 7 days (all P < 0.05). rhAAT-Fc without elastase inhibiting activity, rhAAT-Fc[E−], conferred comparable effects on infarct size, caspase-1 activity, and LVEF (P > 0.2 vs. rhAAT-Fc).

Conclusions:
The pAAT and recombinant human AAT-Fc reduce the acute myocardial inflammatory injury after ischemia–reperfusion in the mouse leading to preservation of viable myocardium and systolic function, independent on the effects on neutrophil elastase.