bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Sequence of
Enterococcus mundtii
EM01, Isolated from
Bombyx mori
Midgut and Responsible for Flacherie Disease in Silkworms Reared on an Artificial Diet
Authors: de Diego-Diaz Beatriz , Treu Laura , Campanaro Stefano , da Silva Duarte Vinicius , Saviane Alessio , Cappellozza Silvia , Squartini Andrea 
Published: January 2018

ABSTRACT

The whole genome sequence of
Enterococcus mundtii
strain EM01 is reported here. The isolate proved to be the cause of flacherie in
Bombyx mori
. To date, the genomes of 11 other
E. mundtii
strains have been sequenced. EM01 is the only strain that displayed active pathological effects on its associated animal species.
Draft Genome Sequence of
Fusarium fujikuroi
, a Fungus Adapted to the Fuel Environment
Authors: Radwan Osman , Gunasekera Thusitha S. , Ruiz Oscar N. 
Published: January 2018

ABSTRACT

Fusarium fujikuroi
isolate FUS01 is highly adapted to grow in jet fuel with predicted genes involved in hydrocarbon catabolism and carbon assimilation. The draft genome size is estimated at 49 Mb containing 18,578 proteins with high similarity to that of
F. fujikuroi
isolate B14.
Complete Genome Sequences of Three
Moraxella osloensis
Strains Isolated from Human Skin
Authors: Lim Jae Yun , Hwang Ingyu , Ganzorig Munkhtsatsral , Huang Shir-Ly , Cho Gyu-Sung , Franz Charles M. A. P. , Lee Kyoung 
Published: January 2018

ABSTRACT

Here, we present the complete whole-genome sequences of three
Moraxella osloensis
strains with octylphenol polyethoxylate-degrading abilities. These strains were isolated from human skin.
Cellular and Humoral Immunity Protect against Vaginal Zika Virus Infection in Mice
Authors: Scott Jason M. , Lebratti Tania J. , Richner Justin M. , Jiang Xiaoping , Fernandez Estefania , Zhao Haiyan , Fremont Daved H. , Diamond Michael S. , Shin Haina 
Published: January 2018

ABSTRACT
Zika virus (ZIKV), which can cause devastating disease in fetuses of infected pregnant women, can be transmitted by mosquito inoculation and sexual routes. Little is known about immune protection against sexually transmitted ZIKV. In this study, we show that previous infection through intravaginal or subcutaneous routes with a contemporary Brazilian strain of ZIKV can protect against subsequent intravaginal challenge with a homologous strain. Both routes of inoculation induced high titers of ZIKV-specific and neutralizing antibody in serum and the vaginal lumen. Virus-specific T cells were recruited to and retained in the female reproductive tract after intravaginal and subcutaneous ZIKV infection. Studies in mice with genetic or acquired deficiencies in B and/or T cells demonstrated that both lymphocyte populations redundantly protect against intravaginal challenge in ZIKV-immune animals. Passive transfer of ZIKV-immune IgG or T cells significantly limited intravaginal infection of naive mice, although antibody more effectively prevented dissemination throughout the reproductive tract. Collectively, our experiments begin to establish the immune correlates of protection against intravaginal ZIKV infection, which should inform vaccination strategies in nonpregnant and pregnant women.

IMPORTANCE
The recent ZIKV epidemic resulted in devastating outcomes in fetuses and may affect reproductive health. Unlike other flaviviruses, ZIKV can be spread by sexual contact as well as a mosquito vector. While previous studies have identified correlates of protection for mosquito-mediated infection, few have focused on immunity against sexual transmission. As exposure to ZIKV via mosquito bite has likely occurred to many living in areas where ZIKV is endemic, our study addresses whether this route of infection can protect against subsequent sexual exposure. We demonstrate that subcutaneous ZIKV infection can protect against subsequent vaginal infection by generating both local antiviral T cell and antibody responses. Our research begins to define the immune correlates of protection for ZIKV infection in the vagina and provides a foundation for testing ZIKV vaccines against sexual transmission.
Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK) for Fuchs endothelial corneal dystrophy: 6 months clinical outcome
Authors: Zygoura Vasiliki , Baydoun Lamis , Ham Lisanne , Bourgonje Vincent J A , van Dijk Korine , Lie Jessica T , Dapena Isabel , Oellerich Silke , Melles Gerrit R J 
Published: January 2018

Background/aimTo assess the clinical outcome of the first series of Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK), a potential hybrid technique between ‘descemetorhexis only’ and conventional, circular DMEK.MethodsProspective interventional case series at a tertiary referral centre. Twelve eyes of 12 patients with central Fuchs endothelial corneal dystrophy underwent Quarter-DMEK, that is, transplantation of one quadrant of a full-diameter DMEK graft, and were evaluated for best-corrected visual acuity (BCVA), endothelial cell density (ECD) and complications up to 6 months postoperatively.ResultsAt 6 months postoperatively, all eyes reached a BCVA of ≥20/40 (≥0.5), 11/12 (92%) of ≥20/25 (≥0.8) and 6/12 (50%) of ≥20/20 (≥1.0). Mean central ECD decreased from 2867 (±161) cells/mm2before to 1255 (±514) cells/mm2at 1 month, 1058 (±455) cells/mm2at 3 months and 968 (±427) cells/mm2at 6 months after surgery. Rebubbling was performed in 4/12 eyes (33%) within the first two months.ConclusionsQuarter-DMEK may be a feasible procedure that allows for visual outcomes similar to conventional, circular DMEK. The relatively large drop in ECD within the first month may have resulted from more extensive endothelial cell migration and/or measurement error (at the graft edges). If longer-term outcomes would resemble those of conventional DMEK, Quarter-DMEK may potentially quadruple the availability of endothelial grafts.
Enhanced gas recovery of an ageing field utilizing N
2
displacement: De Wijk Field, The Netherlands
Authors: Goswami Rajasmita , Seeberger Fritz C. , Bosman Geert 
Published: January 2018

Abstract

Enhancing recovery of the remaining gas in ageing fields presents a commercial opportunity. As such, NAM took the decision to apply on-site generated N
2
injection for the first time. The De Wijk gas field was selected based on size and geological setting, allowing the demonstration of N
2
flooding of depleted gas in diverse reservoir types, as well as testing the efficiency of residual gas sweeping in the watered-out reservoir. De Wijk is one of the oldest onshore natural gas fields in The Netherlands, having produced
c.
15 Bcm (billion cubic metres) of high calorific gas with a natural N
2
content of 5–11% for more than 60 years, bringing it close to the end of field life. Enhanced gas recovery in this field would extend the field life and that of the Ten Arlo gas-processing system, thereby also lengthening tail-end production of other connected fields. In De Wijk, the existing 35 wells were used as dedicated injectors and producers. The production rates from the latter are used to help better understand reservoir flow behaviour. Integration of geological analysis and available production data helped to implement the N
2
enhanced gas recovery technology in this mature field. Two processes of enhanced gas recovery were tested: a gas–gas displacement in the depleted gas leg (Nitrogen Assisted Depletion Drive: NADD); and gas displacing residual gas in the water leg (Nitrogen Enhanced Residual Gas: NERG). Observations after 1 year of N
2
injection showed that planned v. actual performance of the NADD technology is comparable with an increase in production of 100 000–160 000 Nm
3
/day (normal cubic metre per day). NERG application restored a watered-out well; however, further investigation of this application is required. It is demonstrated that a detailed geological understanding of, for example, permeable Rogenstein oolite intervals and the lateral connectivity of internal high-permeability streaks in Volpriehausen sandstones is crucial to the successful application of enhanced gas recovery techniques in such ageing fields.
Evidence for the ISG15-Specific Deubiquitinase USP18 as an Antineoplastic Target
Authors: Mustachio Lisa Maria , Lu Yun , Kawakami Masanori , Roszik Jason , Freemantle Sarah J. , Liu Xi , Dmitrovsky Ethan 
Published: January 2018

AbstractUbiquitination and ubiquitin-like posttranslational modifications (PTM) regulate activity and stability of oncoproteins and tumor suppressors. This implicates PTMs as antineoplastic targets. One way to alter PTMs is to inhibit activity of deubiquitinases (DUB) that remove ubiquitin or ubiquitin-like proteins from substrate proteins. Roles of DUBs in carcinogenesis have been intensively studied, yet few inhibitors exist. Prior work provides a basis for the ubiquitin-specific protease 18 (USP18) as an antineoplastic target. USP18 is the major DUB that removes IFN-stimulated gene 15 (ISG15) from conjugated proteins. Prior work discovered that engineered loss of USP18 increases ISGylation and in contrast to its gain decreases cancer growth by destabilizing growth-regulatory proteins. Loss of USP18 reduced cancer cell growth by triggering apoptosis. Genetic loss of USP18 repressed cancer formation in engineered murine lung cancer models. The translational relevance of USP18 was confirmed by finding its expression was deregulated in malignant versus normal tissues. Notably, the recent elucidation of the USP18 crystal structure offers a framework for developing an inhibitor to this DUB. This review summarizes strong evidence for USP18 as a previously unrecognized pharmacologic target in oncology. Cancer Res; 78(3); 587–92. ©2018 AACR.
O PÚBLICO E O PRIVADO, AS PATENTES E A SAÚDE: ESTUDO DA FRAUDE DE FARMACÊUTICAS NA ITÁLIA COMPARADO À LEGISLAÇÃO BRASILEIRA
Authors: Rocha Cristianne Maria Famer , Petersen Letícia Lassen , Santos Lígia Daiane Fink dos 
Published: January 2018

O presente artigo aborda os obstáculos à regulação econômica do comércio de medicamentos no Brasil. A delimitação temática recai sobre o caso italiano de prática de conluio por parte de duas empresas do ramo farmacêutico para fraudar o sistema de saúde e a existência de indícios da mesma prática no Brasil. A prática do crime contra a ordem econômica e financeira respingou na própria organização do sistema público de saúde italiano e as empresas foram consideradas culpadas pela prática criminosa. O caso retoma a discussão da necessidade de intervenção estatal na economia – do ponto de vista tanto da regulação do mercado quanto da fiscalização do que é efetivamente ofertado à população pelo comércio –, com vistas à promoção da dignidade da pessoa humana, o que inclui a proteção da saúde pelo Estado. A pesquisa tem por objetivo sistematizar o caso italiano de duplicidade no registro de patente, paralelamente abordando a regulação econômica do registro dos medicamentos no Brasil e a criminalização do conluio nessa seara, para, então, testar a hipótese de pesquisa de que a mesma conduta empresarial de duplo registro da patente também foi adotada no Brasil. O método hipotético dedutivo conduz a realização da pesquisa, oferecendo ao leitor a sistematização do caso escolhido e conceitos com base em pesquisa bibliográfica e documental (livros, jornais de repercussão internacional, revistas científicas, artigos científicos, bulas dos medicamentos envolvidos e legislação brasileira sobre a matéria) para, assim, dialogar acerca dos fatos.
ALGUMAS BATALHAS ESTÃO SENDO PERDIDAS. PERDEREMOS A GUERRA?
Authors: Dallari Sueli Gandolfi 
Published: January 2018

Já conversamos neste espaço sobre as disputas ocorridas nos primeiros anosdesta década a respeito dos sistemas públicos universais de saúde e da chamada cobertura sanitária universal. O fato é que a saúde passou a ser tema de interesse na Assembleia Geral da Organização das Nações Unidas (ONU).
Bandeira de Santa Teresa. Ecos da obra de Teresa de Ávila em nove poemas de Manuel Bandeira
Authors: Ferreira Ana 
Published: January 2018

O objetivo do presente artigo é apresentar a hipótese de diálogo da obra do poeta brasileiro Manuel Bandeira com a da santa poeta espanhola Teresa de Ávila. Considerando a colaboração de Manuel Bandeira na tradução da obra de Santa Teresa de Ávila para o português, e a simultaneidade do erótico e o místico recorrente nos versos bandeirianos, buscamos revelar possíveis ecos da obra desta importante expressão da poesia religiosa do Siglo de Oro espanhol em nove poemas de Bandeira, integrantes de livros vários: de Carnaval, publicado em 1919, a Opus 10, de 1952. Na breve análise destes nove poemas de Manuel Bandeira, destacamos possíveis influências da obra de Santa Teresa, na forma, nos termos, símbolos, e até na cronologia, tendo em conta ainda a biografia do poeta modernista. Além da influência literária propriamente dita, Teresa de Ávila parece se desdobrar em objeto de desejo do sujeito lírico, constando em versos ao longo de toda a obra de Bandeira, em disposição que sugere etapas de uma longa relação amorosa.
Design and Verification of a New Monochromatic Thermal Emission Component for the I3RC Community Monte Carlo Model
Authors: Jones Alexandra L. , Di Girolamo Larry 
Published: January 2018

Abstract
The Intercomparison of 3D Radiation Codes (I3RC) community Monte Carlo model has been extended to include a source of photon emission from the surface and atmosphere, thereby making it capable of simulating scalar radiative transfer in a 3D scattering, absorbing, and emitting domain with both internal and external sources. The theoretical basis, computational implementation, verification of the internal emission, and computational performance of the resulting model, the “IMC+emission,” is presented. Thorough verification includes fundamental tests of reciprocity and energy conservation, comparison to analytical solutions, and comparison with another 3D model, the Spherical Harmonics Discrete Ordinate Method (SHDOM). All comparisons to fundamental tests and analytical solutions are accurate to within the precision of the simulations—typically better than 0.05%. Comparison cases to SHDOM were typically within a few percent, except for flux divergence near cloud edges, where the effects of grid definition between the two models manifest themselves. Finally, the model is applied to the established I3RC case 4 cumulus cloud field to provide a benchmark result, and computational performance and strong and weak scaling metrics are presented. The outcome is a thoroughly vetted, publicly available, open-source benchmark tool to study 3D radiative transfer from either internal or external sources of radiation at wavelengths for which scattering, emission, and absorption are important.
The Tomb of Maya and Meryt: Preliminary Report on the Saqqâra Excavations, 1987–8
Authors: Martin Geoffrey T. , Raven Maarten J. , Aston Barbara Greene , Van Dijk Jacobus 
Published: January 2018

During two seasons of work the EES-Leiden expedition excavated the tomb of Maya and Meryt. It is similar to the neighbouring tomb of Horemheb, except that the outer court is not furnished with a complete peristyle. The reveals of the pylon are decorated with reliefs to a height of 3.33 m, including scenes with Maya adoring Osiris, offering bearers, and an autobiographical text. The main courtyard appears to be unfinished. More reliefs were found in the inner court and on the reveals of the entrances to the cult chapel and flanking chapels. Some of these were recorded by Lepsius during his 1843 expedition, but some have since disappeared. The substructure is on two levels. The upper was reused for late burials, but the lower is exclusively the burial complex of Maya and his wife. Three finely decorated rooms survive here, though much was pulled down or smashed by ancient plunderers. New light is thrown on the mortuary cult of the tomb owners, and among the surviving textual material is a new hymn to Osiris. Many objects and enormous quantities of closely contexted pottery, some with hieratic dockets, were recovered. The skeletal remains will be examined in a forthcoming season.
Association of CXCL13 serum level and ultrasonographic findings of joints in patients with systemic lupus erythematosus and Jaccoud’s arthropathy
Authors: Ribeiro D Sá , Lins C F , Galvão V , Santos W G Dourado , Rosa G , Machicado V , Pedreira A L , da Fonseca E P , Souza A P Mota Duque , Baleeiro C , Ferreira L G , Oliveira I Silva de , Gama da Silva J P Cotrim , Atta A M , Santiago M B 
Published: January 2018

Objectives The objective of this paper is to perform an ultrasonography (US) analysis of hands and wrists in two groups of patients with systemic lupus erythematosus (SLE), with and without Jaccoud’s arthropathy, matched by age and disease duration and to correlate them with levels of CXCL13 clinical features, laboratory tests and disease activity score. Methods Sixty-four patients with SLE were enrolled, 32 with and 32 without Jaccoud’s arthropathy. Each patient underwent physical examination, laboratory tests (including CXCL13 by ELISA) and bilateral US. Synovial hypertrophy, tenosynovitis and erosions were evaluated according to a semiquantitative grading system with a 0–3 rating. US findings were correlated with serum levels of CXCL13, other serological parameters and disease activity index. Results Synovitis was found in 25/64 patients (39%) and tenosynovitis in 14/64 (22%). These findings were more frequent in SLE patients with Jaccoud’s arthropathy, particularly tenosynovitis ( p = 0.002) and synovitis ( p = 0.01). Median serum level of CXCL13 was 20.16 pg/ml in the whole population (23.21 pg/ml in the Jaccoud’s arthropathy group and 11.48 pg/ml in the group without). There was an association between the presence of disease activity and high level of CXCL13 ( p = 0.004). However, no association was found between high levels of CXCL13 and “arthritis” in SLEDAI, swollen joints on physical examination or synovitis on US. Conclusions US findings in joints of SLE patients with Jaccoud’s arthropathy confirm that synovitis and tenosynovitis are common in these patients. In addition, serum level of CXCL13 is associated with disease activity in SLE but does not seem to be a biomarker for arthritis in these patients.
RETRACTED ARTICLE: TIPE2 suppresses progression and tumorigenesis of esophageal carcinoma via inhibition of the Wnt/β-catenin pathway
Authors: Zhu Linan , Zhang Xudong , Fu Xiaorui , Li Zhaoming , Sun Zhenchang , Wu Jingjing , Wang Xinhua , Wang Feng , Li Xiangke , Niu Songtao , Ding Mengjie , Yang Zhenzhen , Yang Wanqiu , Yin Meifeng , Zhang Lei , Zhang Mingzhi 
Published: January 2018

Abstract
Background
Esophageal carcinoma is the eighth prevalent malignancy and ranks the sixth in carcinoma-related death worldwide. Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2) has been identified as a tumor suppressor in multiple carcinomas. However, its roles and molecular mechanisms underlying esophageal carcinoma progression are still undefined till now.

Methods
RT-qPCR assay was employed to detect the expression of TIPE2 mRNA. TIPE2 protein expression was measured by using western blot assay. Ad-V and Ad-TIPE2 adenoviruses were constructed to overexpress TIPE2. The effects of TIPE2 overexpression on cell proliferation, invasion and apoptosis were assessed by MTT and Edu incorporation assays, transwell invasion assay and flow cytometry analysis, respectively. The effect of TIPE2 overexpression on xenograft tumor growth was determined by measuring tumor volume and weight, together with immunohistochemistry assay. The effect of TIPE2 overexpression on the Wnt/β-catenin signaling pathway was evaluated by detecting the protein levels of β-catenin, c-Myc and cyclinD1 in EC9076 cells and xenograft tumors of esophageal carcinoma.

Results
TIPE2 expression was downregulated in esophageal carcinoma tissues and cells. Adenovirus-mediated TIPE2 overexpression suppressed cell proliferation and invasion, and induced apoptosis in esophageal carcinoma cells. Enforced expression of TIPE2 inhibited tumor growth in vivo, as evidenced by the reduced tumor volume, tumor weight and proliferating cell nuclear antigen expression. Overexpression of TIPE2 inhibited the Wnt/β-catenin signaling pathway in esophageal carcinoma in vitro and in vivo.

Conclusions
These results suggest that TIPE2 suppressed progression and tumorigenesis of esophageal carcinoma via inhibition of the Wnt/β-catenin pathway.
Impact of Geographic Regions on Overall Survival in Patients With Metastatic Renal Cell Carcinoma: Results From an International Clinical Trials Database
Authors: Fay Andre P. , McKay Rana R. , Lin Xun , Simantov Ronit , Choueiri Toni K. 
Published: January 2018

Purpose Health determinants vary according to geographic region and may affect the outcomes of patients with metastatic renal cell carcinoma (mRCC) treated during clinical trials of targeted therapy. Here, we investigate the overall survival (OS) of patients with mRCC treated in the era of targeted therapy by geographic region. Methods We conducted a pooled analysis of patients with mRCC who were treated during phase II or III clinical trials. Clinical characteristics and survival data were collected. Statistical analyses were performed with the Kaplan-Meier method and log-rank test in univariable analysis. Results Overall, 4,736 patients were included in the analysis. Patient characteristics differed according to geographic region. No statistically significant differences in OS were observed when the United States/Canada (USC) was compared with the following other regions: Latin America, Asia/Oceania/Africa, and Eastern Europe. In a univariable analysis, OS differed among patients enrolled in trials in USC compared with Western Europe (20.3 v 17.4 months; hazard ratio, 1.15; 95% CI, 1.03 to 1.3; P = .015), but it did not differ in a multivariable analysis. All-grade treatment-related adverse events (AEs) were observed more frequently in USC. There were no significant differences in grade 3 to 5 AEs among groups. Conclusion Despite different baseline characteristics, OS was similar among patients enrolled in clinical trials across different geographic regions. Access to clinical trials as well as disease biology, AE reporting, and quality of care may contribute to potential differences in outcomes.
Silacrown Ethers-Clay Intercalation Materials: Application in Potentiometric Sensors for Detection of Alkali-Ions
Authors: Ruiz-Hitzky Eduardo , Gómez-Avilés Almudena , Darder Margarita , Aranda Pilar 
Published: January 2018

Abstract
Dimethylsila-14-crown-5 (DMS14C5) and dimethylsila-17-crown-6 (DMS17C6) macrocyclic polyethers have been intercalated into montmorillonite layered silicates containing Li+, Na+ or K+ cations as exchangeable cations. XRD and FTIR spectroscopy offer information on the disposition of the macrocycle in the interlayer and accommodation of cations within the cavity of the polyoxyethylene ligand. 13C and 23Na solid-state NMR spectroscopy provides information about the replacement of water molecules from the hydration shell of interlayer cations by the macrocyclic ligand and confirms differences in the interactions depending on the nature of the ligand. The resulting montmorillonite intercalation materials were tested as active phase of PVC electrodes for the development of potentiometric sensors, in a preliminary attempt to evaluate their electrochemical response towards alkali-metal ions in solution.