bluuebunny/crossref_metadata_2025_split huggingface dataset:
Edible insects: A survey about perceptions regarding possible beneficial health effects and safety concerns among adult citizens from Portugal and Romania
Authors: Guiné Raquel P. F. , Florença Sofia G. , Costa Cristina A. , Correia Paula M. R. , Ferreira Manuela , Cardoso Ana P. , Campos Sofia , Anjos Ofélia , Bacarea Vladimir , Bacarea Anca , Buicu Gabriela , Szasz Simona , Martin-Hadmaș Roxana , Tarcea Monica 
Published: September 2024

Abstract
Although edible insects (EI) have been consumed for ages in many countries around the world, in most European countries, this is not a traditional practice. Still, it has been encouraged as a sustainable source of animal protein besides other nutrients. The Food and Agricultural Organization and the United Nations, recommend consuming EI as a way to contribute to the sustainable development goals. Hence, this research investigated how participants from Portugal and Romania perceive EI, and how these can be useful as a source of nutrients and other benefits for human health, as well as possible risks. For that, it was undertaken a questionnaire survey, with data collected online in the two countries. For the analysis of data, basis statistics and chi-square tests were used. Results showed significant differences between Portuguese and Romanian participants in six out of ten questions, namely regarding safety (p < 0.001), use in traditional medicine (p < 0.001), possible infection with parasites (p = 0.021), of pesticides (p = 0.031), their usage as therapeutics (p = 0.001) and being potential sources of allergens (p < 0.001) and aflatoxins (p < 0.001). Also, differences were tested for other sociodemographic variables. The results showed significant differences according to age for only three questions, gender for only two questions, and Education for most of the questions, i.e., six out of the ten. These results highlighted the greater influence of variables like country of origin and education in the perception of the effects of EI in human health.
The efficacy and functional consequences of interactions between human spermatozoa and seminal fluid extracellular vesicles
Authors: Tamessar Cottrell T , Anderson Amanda L , Bromfield Elizabeth G , Trigg Natalie A , Parameswaran Shanmathi , Stanger Simone J , Weidenhofer Judith , Zhang Hui-Ming , Robertson Sarah A , Sharkey David J , Nixon Brett , Schjenken John E 
Published: September 2024

Abstract
Seminal fluid extracellular vesicles (SFEVs) have previously been shown to interact with spermatozoa and influence their fertilisation capacity. Here, we sought to extend these studies by exploring the functional consequences of SFEV interactions with human spermatozoa. SFEVs were isolated from the seminal fluid of normozoospermic donors prior to assessing the kinetics of sperm-SFEV binding in vitro, as well as the effects of these interactions on sperm capacitation, acrosomal exocytosis, and motility profile. Biotin-labelled SFEV proteins were transferred primarily to the flagellum of spermatozoa within minutes of co-incubation, although additional foci of SFEV biotinylated proteins also labelled the mid-piece and head domain. Functional analyses of high-quality spermatozoa collected following liquefaction revealed that SFEVs did not influence sperm motility during incubation at pH 5, yet SFEVs induced subtle increases in total and progressive motility in sperm incubated with SFEVs at pH 7. Additional investigation of sperm motility kinematic parameters revealed that SFEVs significantly decreased beat cross frequency and increased distance straight line, linearity, straightness, straight line velocity, and wobble. SFEVs did not influence sperm capacitation status or the ability of sperm to undergo acrosomal exocytosis. Functional assessment of both high- and low-quality spermatozoa collected prior to liquefaction showed limited SFEV influence, with these vesicles inducing only subtle decreases in beat cross frequency in spermatozoa of both groups. These findings raise the prospect that, aside from subtle effects on sperm motility, the encapsulated SFEV cargo may be destined for physiological targets other than the male germline, notably the female reproductive tract.

Lay Summary
A male’s influence over the biological processes of pregnancy extends beyond the provision of sperm. Molecular signals present in the ejaculate can influence the likelihood of pregnancy and healthy pregnancy progression, but the identity and function of these signals remain unclear. In this study, we wanted to understand if nano-sized particles present in the male ejaculate, called seminal fluid extracellular vesicles, can assist sperm in traversing the female reproductive tract to access the egg. To explore this, we isolated seminal fluid extracellular vesicles from human semen and incubated them with sperm. Our data showed that seminal fluid extracellular vesicles act to transfer molecular information to sperm, but this resulted in only subtle changes to the movement of sperm.

Graphical abstract
NSUN2 is critical for mouse oocyte meiosis and early embryonic development
Authors: Xu Tengteng , Gao Min , Zhang Ling , Cao Tianqi , Qiu Yanling , Liu Simiao , Wu Wenlian , Zhou Yitong , Liu Haiying , Zhang Rui , Ruan Xiaohong , Huang Junjiu 
Published: September 2024

In brief
The mechanism by which the NSUN2 mutation causes female infertility is still unclear. This study reveals the role and potential mechanism of NSUN2 in mouse oocyte maturation and early embryonic development, and provides a resource for elucidating female infertility with NSUN2 mutations.

Abstract
Biallelic variants in the NSUN2 gene cause a rare intellectual disability and female infertility in humans. However, the function and mechanism of NSUN2 during mouse oocyte meiotic maturation and early embryonic development are unknown. Here, we show that NSUN2 is important for mouse oocyte meiotic maturation and early embryonic development. Specifically, NSUN2 is required for ovarian development and oocyte meiosis, and deletion of Nsun2 reduces oocyte maturation and increases the rates of misaligned chromosomes and aberrant spindles. In addition, Nsun2 deficiency results in a low blastocyst rate and impaired blastocyst quality. Strikingly, loss of Nsun2 leads to approximately 35% of embryos being blocked at the 2-cell stage, and Nsun2 knockdown impairs zygotic genome activation at the 2-cell stage. Taken together, these findings suggest that NSUN2 plays a critical role in mouse oocyte meiotic maturation and early embryonic development, and provide key resources for elucidating female infertility with NSUN2 mutations.
La Confianza de los Colombianos en la Gestión Política del Recurso Público como indicador de un ambiente democrático
Authors: Gómez Betancur Milany , Gómez Ramírez Sara , Tibaquira Castrillon Elizabeth 
Published: September 2024

El presente trabajo tiene como objetivo analizar hasta qué punto confían los colombianos en el uso del recurso público y si esto tiene relación con la percepción que tienen del ambiente democrático en el país. Dicha investigación hace parte del macroproyecto titulado “Percepciones sobre la democracia y confianza política en Colombia 2022” que buscó analizar diversas actitudes, percepciones y practicas ciudadanas que influyen en la construcción o no de un ambiente democrático en Colombia. Así, para lograr el objetivo del presente trabajo, se usó una metodología mixta donde se hizo en primera instancia un análisis documental que posibilitara determinar qué se entiende por ambiente democrático y por un correcto manejo del recurso público, posteriormente, se aplicó un instrumento demoscópico en las principales ciudades de Colombia (Bogotá, Medellín, Barranquilla, Cali, Bucaramanga) con un número total de 1000 muestras que luego fueron sistematizas y analizadas a través de un análisis de asociación de variables y correlacional, usando estadísticos como el chi-cudrado, la prueba de Person y el t de Studen. El resultado estadístico indicó no solo que hay una baja confianza de los colombianos en el manejo de los recursos públicos, las instituciones y los líderes que los ejecutan y ordenan el gasto, sino que esto influye realmente en la percepción negativa que tiene del sistema democrático y, por ende, en la garantía de los derechos humanos el cual es la finalidad de un Estado con este sistema. Este tipo de investigaciones hacen un llamado desde la empírica a comprender el fenómeno de la corrupción, y su preocupante implicación para la democracia de la región, es ahí donde radica su originalidad.
ПРОБЛЕМА «ЦИФРОВОЙ ОТМЕНЫ» ГРАЖДАН РОССИИ
Authors: ДИСКИН Евгений Иосифович 
Published: September 2024

Рассмотрен феномен «цифровой отмены» как формы дискриминации на примере нарушения прав граждан России путем скоординированного и синхронного прекращения оказания цифровых информационных услуг и сервисов со стороны иностранных компаний и цифровых сервисов, а также установления прямого запрета на доступ к таким услугам и сервисам. Многочисленные примеры массовых запретов и ограничений, которые введены в отношении граждан России в интернете на уровне социальных сетей, финансовых сервисов, сервисов доступа к онлайн-играм, программному обеспечению, сервисам для работы с искусственным интеллектом, позволяют сделать вывод, что такие действия являются дискриминацией и нарушением прав человека на свободный доступ к получению, сбору и распространению информации. Предлагается рассматривать такие действия в рамках международно-правовых документов, запрещающих дискриминацию по национальному признаку, в том числе в целях обращения в международные судебные органы с соответствующими судебными исками, которые могут быть поданы Российской Федерацией.
Adaptation of Geomatics Engineering Teacher of Dual Expertise Program to New Skill Competencies
Authors: Yavis David , Herman Nanang Dalil 
Published: September 2024

Research on eleven participants who work as teachers of the Geomatics Engineering Dual Expertise Program spread across various provinces throughout Indonesia has provided an in-depth desciption of the adaptation process they experienced to their new competency skills. The author conducts interviews with video calls through applications on sosial media by giving several questions to participants and also asking participants to tell about the adaptation process they have experienced so that the results obtained from this interview are more in-depth. The author processes the research data by transcribing the document, compressing the facts, then coding so that the conclusion is in the form of certain themes that can be grouped. From this research, the authors have succeeded in describing the main obstacles experienced by teachers in adapting and how they can overcome these obstacles in their way. This research shows that some of the obstacles for the geomatics engineering dual expertise program teacher in adapting to this skill competency are understanding new subject matter in the competency of geomatics engineering expertise, namely Geographic Information Systems (GIS) and Remote Sensing (RS) which are closely related to learning using software certainly. Inadequate facilities and infrastructure such as practical equipment for teaching and learning activities, lack of support from senior teachers in schools. The participants tried to overcome the obstacles they experienced with the shocking reality because they avoided teaching difficult subjects (subjects in Grade XI and XII) or tried to gain knowledge with senior teachers in school, even though not all senior teachers can provide support due to limited understanding of new material.
Nexus between soft skills and technical vocational education and training graduate employability
Authors: Awodiji Omotayo Adewale , Magogodi Coleen 
Published: September 2024

Soft skills are increasingly seen as being crucial for the modern workforce due to the rapid and pervasive digitalisation that has transformed the nature of employment. To work with new technologies and stay up with the rapid changes in technology, graduates require employability skills. Despite the growing demand there remains a shortage of employability skills, organisations frequently struggle to identify qualified candidates for digital jobs because workforces frequently lack the skills necessary to manage digital change. Soft skills became more important as the pace of world of work moved online. Meanwhile, the role of TVET in job creation cannot be over emphasised in the changing world. Thus, the study examined how soft skills can enhance TVET graduates’ employability. Study participants included 351 students from Botswana TVET schools. To better understand how soft skills relate to TVET graduates' employability, we used STATA software to establish the correlation between the two variables. The result indicated that soft skills promote TVET graduates' employability. Hence, all TVET administrators should keep up-to-date with this present model so that they can design policies that promote employability for TVET graduates.
Pediatric follicular lymphoma: literature review and presentation of a rare clinical case
Authors: Volkova A. S. , Valiev T. T. , Abramov D. S. , Tarakanova A. V. , Odzharova A. A. , Ryabukhina Yu. E. , Zeynalova P. A. 
Published: September 2024

Follicular lymphoma is one of the most common non-Hodgkin’s lymphomas in adults. One of the rather rare variants of follicular lymphoma is pediatric follicular lymphoma. This variant, despite the name, is diagnosed not only in children, but also among young adults. Pediatric follicular lymphoma is characterized by early (I, II) stages, the absence or weak BCL2 expression, and the predominant absence of t(14;18)(q32;q21) translocation. Treatment tactics vary widely from radical surgical tumor resection with subsequent follow-up to chemoimmunotherapy with rituximab.This article presents a clinical case of advanced pediatric follicular lymphoma (stage III) in a 5-year-old patient. Using a combined treatment approach (surgery followed by immunochemotherapy) allowed to achieve a complete metabolic response which lasts more than a year.
Trisomy 14 as the only cytogenetic abnormality in myelodysplastic syndrome
Authors: Pautova M. N. , Koloskova L. E. , Filippova O. I. , Koloskov A. V. 
Published: September 2024

Evaluation of bone marrow cytogenetic abnormalities in myelodysplastic syndrome is of great importance for confirming the clonal disease nature, determining the prognosis and choosing treatment tactics. Cytogenetic abnormalities are detected in 40–70 % of patients with myelodysplastic syndrome, and the variety of these abnormalities reflects the disease characteristics.This article describes the clinical follow-up of a patient with a myelodysplastic neoplasia with blast excess 1 and trisomy of chromosome 14.
Clinical profile and therapeutic aspects of mycosis fungoides: a retrospective analysis of 210 cases in Russia
Authors: Gorenkova L. G. , Zvonkov E. E. , Mangasarova Ya. K. , Chabaeva Yu. A. , Kulikov S. M. , Kovrigina A. M. , Kuzmina L. A. , Sidorova Yu. V. , Mozdon M. A. 
Published: September 2024

Background. Mycosis fungoides (MF) is classified as an orphan disease. Due to the rarity of pathology, and until recently the absence of an expert group and a specialized reference center for cutaneous lymphomas in Russia, possible treatment options for MF are presented by listing them without recommendations on the preferred indications for one or another option. This creates difficulties in choosing treatment methods and assessing their effectiveness.Aim. To characterize current treatment methods and their results in MF patients who were observed or received consultative and diagnostic care at the National Medical Research Center for Hematology.Materials and methods. The study included 210 patients: 115 with early disease stages and 95 with advanced stages.Results and conclusion. The most common treatment options were for early stages – local therapy, interferon therapy and systemic chemotherapy (CT), for advanced stages – combination therapy with interferon (+ PUVA therapy, methotrexate), interferon monotherapy and systemic CT. The frequency of systemic chemotherapy use in all lines of MF treatment was 21 %. When integrating statistical analysis using the probability of achieving an antitumor response, switching to 2nd line therapy, and accumulated incidence, the negative results of using chemotherapy in the MF treatment were clearly demonstrated.For the first time in Russia, a real practical situation of the applied MF treatment options is presented on our own large sample of patients. As the first line of therapy, the most common options were immunotherapy and phototherapy, however, in 12.4 % of cases, the use of systemic CT was registered, which is unjustified and leads to a decrease in the time to the next line of treatment and an increase in the cumulative incidence of adverse events. As a result of the use of non-chemotherapeutic approaches (interferon, etc.), the 3-year relapse-free survival rate is about 40 %, after chemotherapy – 9.4 %. Secondand third-line therapy provided more varied options, including combination treatment with interferon and methotrexate, as well as gemcitabine monotherapy, targeted therapy with brentuximab vedotin, and epigenetic therapy in the 3rd line. Studies with targeted agents in this patient population have demonstrated improved clinical outcomes, highlighting the need for their early use to achieve the best results.
Pain syndrome in multiple myeloma (results of a single-center study)
Authors: Soloveva M. V. , Solovev M. V. , Irugova E.  Z. , Startsev A. A. , Arutyunyan N. K. , Krayzman A. A. , Abakumova A. V. , Mendeleeva L. P. 
Published: September 2024

Background. One of the most common symptoms of multiple myeloma (MM) is pain. Bone pain is observed in 60– 80 % of patients at the disease onset. Neuropathic pain syndrome is also often found in MM.Aim. To characterize the pain syndrome in MM at the disease onset and various therapy stages.Materials and methods. From January 2019 to October 2021 a retrospective single-center study included 105 patients with newly diagnosed symptomatic MM (49 men, 56 women) aged from 26 to 83 years (median 58.5). Induction therapy in all patients was performed with bortezomib-containing regimens. High-dose chemotherapy with autologous hematopoietic stem cell transplantation (auto-HSCT) was performed in 44 patients. The Fisher–Freeman test was used to analyze contingency tables.Results. Pain syndrome of varying severity at the onset of MM was observed in 83 % of patients. The median time from the onset of pain to the diagnosis of MM was 120 days. In 62.5 % of patients with kidney damage and pain, analgesics (mainly nonsteroidal anti-inflammatory drugs) were used before the diagnosis of MM. In patients with pain syndrome, compared with patients without it, at the onset of MM, pathological fractures (p = 0.01), bone plasmacytomas (p = 0.0001), hypercalcemia (p = 0.03) were significantly more often detected, and stage III was diagnosed according to Durie– Salmon (p = 0.021). The incidence of peripheral toxic polyneuropathy was 35 %. Complete regression of polyneuropathy symptoms was observed in 19 % of patients, and a significant decrease – in another 62 % of cases. The main manifestation of pain syndrome during auto-HSCT was pain in the oral cavity due to mucositis of varying severity.Conclusion. Our study showed that MM patients mainly with stage III (86 % of cases) are referred for hospitalization to the National Medical Research Center for Hematology. Moreover, in 83 % of them the disease is accompanied by severe pain. More than a third of patients (35 %) developed bortezomib-induced peripheral polyneuropathy. Opioid analgesics are used for pain relief in the hospital, the indications for which were recorded in 45 % and 41 % of patients with MM during induction therapy and auto-HSCT, respectively.
Immunological features of the bone marrow microenvironment and assessment of bone marrow lesions in patients with classical Hodgkin lymphoma
Authors: Melnikova A. A. , Abbasbeyli F. M. , Mushkarina T. Yu. , Vernyuk M. A. , Zeynalova P. A. , Grivtsova L. Yu. , Ivanov S. A. , Kaprin A. D. 
Published: September 2024

Background. The study of bone marrow involvement is a necessary step in disease staging, both in non-Hodgkin lymphomas and in the case of Hodgkin lymphoma (HL). The standard assessment of bone marrow lesion is carried out based on immunohistochemical examination of bone marrow trepanobioptate, and recently computer technologies (positron emission tomography combined with computed tomography) have been introduced to assess bone marrow involvement. At the same time, immunological methods for detecting tumor cells using multicolor flow cytometry are more available. In addition, they allow for detailed study of the tumor bone marrow microenvironment.Aim. To evaluate the immunomorphological features of bone marrow in HL and the possibility of identifying specific bone marrow lesions using flow cytometry.Materials and methods. The study included the analysis of 107 bone marrow samples. The samples were obtained from 107 patients with various variants of classical HL, diagnosed and treated at the Lapino Clinical Hospital and the P.A. Herzen Moscow State Medical Institute from 2018 to 2022.Results. The features of bone marrow lymphocytes subpopulation in patients with classical HL are shown.Conclusion. An immunological assessment of bone marrow involvement degree was carried out, data from flow cytometry and immunohistochemical examination of trephine biopsy specimens during primary diagnosis were compared, and the features of bone marrow lymphocytes subpopulation were evaluated. The interrelation of these factors at the diagnostic stage in classical variants of HL was studied. At the end of treatment, the primary immunological data (the degree of bone marrow involvement) were compared with the results of the positron emission tomography combined with computed tomography.
Reply to “Comment on ‘An Evaluation of the Timing Accuracy of Global and Regional Seismic Stations and Networks’ by Yang et al. (2021)” by Xin Zhang and Lianxing Wen
Authors: Yang Yi , Song Xiaodong , Ringler Adam T. 
Published: September 2024

Abstract
The finding of Yang et al. (2021; hereafter, YSR21) casts fundamental doubts on all the previous studies on the temporal changes of the inner core (IC) surface that are based on absolute arrival times, including Wen (2006; hereafter, WEN06) and its follow-up studies. The reports of small time shifts of the IC-reflected phase (PKiKP) likely misinterpreted station-related issues (clock errors and instrument changes) as evidence for rapid localized deformation at the IC surface. The comment by Zhang and Wen (2024; hereafter, ZW24) aims at defending the results and conclusions of WEN06 by questioning a tiny portion of the results (2 out of over 400 doublets and 3 out of over 400 problematic stations) in YSR21. In response, we acknowledge an accidental error in table S1 of YSR21, which is corrected in an erratum (Yang et al., 2023), although this does not affect the results or interpretations of YSR21. However, we strongly disagree with the criticisms in ZW24 due to many problems therein, especially the issue of irreproducibility due to its inconsistent procedures with YSR21. While the problems are related to the use of absolute arrivals in the previous studies by Wen’s group, key problems in this new comment are related to questionable data selection and inconsistent processing. We further caution the metadata updates, particularly when involving small temporal change signals. We focus on the main points in ZW24 and demonstrate that its arguments are problematic in many aspects and do not diminish the credibility of YSR21 or other related works by Yang and Song in support of the IC differential rotation.