bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybridized gravitational search algorithm tuned sliding mode controller design for load frequency control system with doubly fed induction generator wind turbine
Authors: Dahiya Preeti , Sharma Veena , Naresh R. 
Published: January 2017

SummaryIn this paper, the load frequency regulation problem of 2‐area interconnected power system is resolved using the sliding mode control (SMC) methodology. Interconnected 2‐area power systems with and without doubly fed induction generator wind turbines are considered for implementing the proposed optimal control methodology. Here, a heuristic gravitational search algorithm (GSA) and its variants such as opposition learning–based GSA (OGSA), disruption‐based GSA (DGSA), and disruption based oppositional GSA (DOGSA) are employed to optimize the switching vector and feedback gains of SMC. In order to overcome the inherent chattering problem in SMC, the control signals are considered in the objective function. The robustness of optimized SMC is analyzed by the inclusion of nonlinearities such as generation rate constraint (GRC), governor deadband, and time delay during the signal processing between the control areas, which are present in the real‐time power system. The insensitiveness of the optimal controller is shown by variation in system parameters like loading condition, speed governor constant, turbine constant, and tie‐line power coefficient. Further, the optimal SMC has been studied with significant load variations and wind power penetration levels in the control areas. The potential of proposed SMC design with chattering reduction feature is shown and validated by comparing the results obtained with the other reported methods in the literature.
The Transferability of Terrestrial Water Balance Components under Uncertainty and Nonstationarity: A Case Study of the Coastal Plain Watershed in the Southeastern USA
Authors: Samadi S. Z. , Meadows M. E. 
Published: January 2017

AbstractsThe challenges posed by nonstationarity in predicting catchment water balance components motivated this study to test the stationary versus nonstationarity hypothesis and detect changes in the watershed response to land use land cover (LULC) alterations, and climate variability and change. The focus is on a two‐step procedure that includes model calibration of Soil and Water Assessment Tool using a sequential Bayesian uncertainty algorithm (i.e. sequential uncertainty fitting), followed by nonstationary assessment of water balance component using extreme value analysis over an Atlantic coastal plain watershed in the southeastern USA. Analysis suggests that the uncertainty of Soil and Water Assessment Tool model is statistically aligned with LULC alterations that increased the sensitivity of Manning's roughness coefficient, transmission loss and the resistance of the soil matrix to water flow. Changes in LULC along with variability in the magnitude, timing and frequency of precipitation diminished surface runoff and groundwater contribution to the river system whereas it increased evapotranspiration with a substantial decline in water storage capacity. Nonstationary assessment of water balance using extreme value analysis model further revealed a functional form of stationary behaviour (no trends) prior to LULC alteration while large amplification was detected during post‐changes. The results and findings presented in this paper confirm our hypothesis about a combined effect of climate and LULC changes on hydrological functions and that variation of these fingerprints elucidates the presence of nonstationarity in the watershed system. Copyright © 2017 John Wiley & Sons, Ltd.
Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells
Authors: Sontag Stephanie , Förster Malrun , Qin Jie , Wanek Paul , Mitzka Saskia , Schüler Herdit M. , Koschmieder Steffen , Rose-John Stefan , Seré Kristin , Zenke Martin 
Published: January 2017

Abstract
Human induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers, including hematopoietic stem cells and their progeny. Interferon regulatory factor 8 (IRF8) is a transcription factor, which acts in hematopoiesis as lineage determining factor for myeloid cells, including dendritic cells (DC). Autosomal recessive or dominant IRF8 mutations occurring in patients cause severe monocytic and DC immunodeficiency. To study IRF8 in human hematopoiesis we generated human IRF8−/− iPS cells and IRF8−/− embryonic stem (ES) cells using RNA guided CRISPR/Cas9n genome editing. Upon induction of hematopoietic differentiation, we demonstrate that IRF8 is dispensable for iPS cell and ES cell differentiation into hemogenic endothelium and for endothelial-to-hematopoietic transition, and thus development of hematopoietic progenitors. We differentiated iPS cell and ES cell derived progenitors into CD141+ cross-presenting cDC1 and CD1c+ classical cDC2 and CD303+ plasmacytoid DC (pDC). We found that IRF8 deficiency compromised cDC1 and pDC development, while cDC2 development was largely unaffected. Additionally, in an unrestricted differentiation regimen, IRF8−/− iPS cells and ES cells exhibited a clear bias toward granulocytes at the expense of monocytes. IRF8−/− DC showed reduced MHC class II expression and were impaired in cytokine responses, migration, and antigen presentation. Taken together, we engineered a human IRF8 knockout model that allows studying molecular mechanisms of human immunodeficiencies in vitro, including the pathophysiology of IRF8 deficient DC.
Differentiation of Islet Progenitors Regulated by Nicotinamide into Transcriptome-Verified β Cells That Ameliorate Diabetes
Authors: Jiang Fang-Xu , Li Kevin , Archer Michael , Mehta Munish , Jamieson Emma , Charles Adrian , Dickinson Jan E. , Matsumoto Masahito , Morahan Grant 
Published: January 2017

Abstract
Developmental stage-specific differentiation of stem or progenitor cells into safe and functional cells is of fundamental importance in regenerative medicine, including β-cell replacement. However, the differentiation of islet progenitor cells (IPCs) into insulin-secreting β cells remains elusive. Here, we report that the multifunctional molecule nicotinamide (NIC) is a specific differentiation regulator of mouse IPCs. The differentiated cells regulated by NIC exhibited many characteristics of adult β cells, including ameliorating preclinical diabetes and a highly comparable transcriptome profile. Gene set enrichment analysis showed that during differentiation, numerous IPC transcription factor genes, including Ngn3, Pax4, Fev, and Mycl1, were all down regulated. Pharmacological, biochemical, and gene knockdown analyses collectively demonstrated that NIC regulated the differentiation via inhibiting Sirt1 (silent information regulator transcript 1). Finally, NIC also regulates human IPC differentiation. Thus, our study advances islet developmental biology and impacts on translational research and regenerative therapies to diabetes and other diseases.
A Safety Checkpoint to Eliminate Cancer Risk of the Immune Evasive Cells Derived from Human Embryonic Stem Cells
Authors: He Jingjin , Rong Zhili , Fu Xuemei , Xu Yang 
Published: January 2017

Abstract
Human embryonic stem cells (hESCs) hold great promise in the regenerative therapy of many currently untreatable human diseases. One of the key bottlenecks is the immune rejection of hESC-derived allografts by the recipient. To overcome this challenge, we have established new approaches to induce immune protection of hESC-derived allografts through the coexpression of immune suppressive molecules CTLA4-Ig and PD-L1. However, this in turn raises a safety concern of cancer risk because these hESC-derived cells can evade immune surveillance. To address this safety concern, we developed a safety checkpoint so that the immune evasive hESC-derived cells in the graft can be effectively eliminated if any cellular transformation is detected. In this context, we knock-in the suicidal gene herpes simplex virus thymidine kinase (HSVTK) into the constitutive HPRT locus of CP hESCs (knock-in hESCs expressing CTLA4-Ig and PD-L1), denoted CPTK hESCs. Employing humanized mice (Hu-mice) reconstituted with human immune system, we demonstrated that the CPTK hESC-derived cells are protected from immune rejection. In addition, CPTK hESC-derived cells can be efficiently eliminated in vitro and in vivo with FDA approved TK-targeting drug ganciclovir. Therefore, this new safety checkpoint improves the feasibility to use the immune evasive hESC-derived cells for regenerative medicine.
Transplantation of CD51+ Stem Leydig Cells: A New Strategy for the Treatment of Testosterone Deficiency
Authors: Zang Zhi Jun , Wang Jiancheng , Chen Zhihong , Zhang Yan , Gao Yong , Su Zhijian , Tuo Ying , Liao Yan , Zhang Min , Yuan Qunfang , Deng Chunhua , Jiang Mei Hua , Xiang Andy Peng 
Published: January 2017

Abstract
Stem Leydig cell (SLC) transplantation could provide a new strategy for treating the testosterone deficiency. Our previous study demonstrated that CD51 (also called integrin αv) might be a putative cell surface marker for SLCs, but the physiological function and efficacy of CD51+ SLCs treatment remain unclear. Here, we explore the potential therapeutic benefits of CD51+ SLCs transplantation and whether these transplanted cells can be regulated by the hypothalamic-pituitary-gonadal (HPG) axis. CD51+ cells were isolated from the testes of 12-weeks-old C57BL/6 mice, and we showed that such cells expressed SLC markers and that they were capable of self-renewal, extensive proliferation, and differentiation into multiple mesenchymal cell lineages and LCs in vitro. As a specific cytotoxin that eliminates Leydig cells (LCs) in adult rats, ethane dimethanesulfonate (EDS) was used to ablate LCs before the SLC transplantation. After being transplanted into the testes of EDS-treated rats, the CD51+ cells differentiated into mature LCs, and the recipient rats showed a partial recovery of testosterone production and spermatogenesis. Notably, a testosterone analysis revealed a circadian rhythm of testosterone secretion in cell-transplanted rats, and these testosterone secretions could be suppressed by decapeptyl (a luteinizing hormone-releasing hormone agonist), suggesting that the transplanted cells might be regulated by the HPG axis. This study is the first to demonstrate that CD51+ SLCs can restore the neuroendocrine regulation of testicular function by physiologically recovering the expected episodic changes in diurnal testosterone serum levels and that SLC transplantation may provide a new tool for the studies of testosterone deficiency treatment.
Measurement of crude-cell-extract glycerol dehydratase activity in recombinantEscherichia coliusing coupled-enzyme reactions
Authors: Sankaranarayanan Mugesh , Seol Eunhee , Kim Yeonhee , Chauhan Ashish Singh , Park Sunghoon 
Published: January 2017

AbstractGlycerol dehydratase (GDHt), which converts glycerol to 3-hydroxypropionaldehyde, is essential to the production of 1,3-propanediol (1,3-PDO) or 3-hydroxypropionic acid (3-HP). A reliable GDHt activity assay in crude-cell extract was developed. In the assay, GDHt converted 1,2-propanediol (1,2-PDO) to propionaldehyde, which was further converted to 1-propionic acid by aldehyde dehydrogenase (KGSADH) or to 1-propanol by yeast-alcohol dehydrogenase (yADH), while the NADH concentration change was monitored spectrophotometrically. Cells should be disintegrated by Bead Beater/French Press, not by chemical methods (BugBuster®/B-PER™), because the reagents significantly inactivated GDHt and coupling enzymes. Furthermore, in the assay mixture, a much higher activity of KGSADH (&gt;200-fold) or yADH (&gt;400-fold) than that of GDHt should have been maintained. Under optimal conditions, both KGSADH and yADH showed practically the same activity. The coupled-enzyme assay method established here should prove to be applicable to recombinant strains developed for the production of 3-HP and/or 1,3-PDO from glycerol.
Does Trainee Involvement in Fluoroscopic Injections Affect Fluoroscopic Time, Immediate Pain Reduction, and Complication Rate?
Authors: Cushman Daniel , Teramoto Masaru , Curtis Bradley , Lee David T. , Marcolina Austin , McCormick Zachary 
Published: January 2017

AbstractBackgroundPatients have expressed concern about undergoing procedures involving trainees, even with direct attending physician supervision. Little literature has examined the effect of trainee involvement on patient outcomes.ObjectiveWe aimed to evaluate the effect of trainee involvement on patient complications, immediate pain reduction, and fluoroscopic time for different fluoroscopic injection types.DesignRetrospective review.SettingFour academic outpatient institutions with Accreditation Council for Graduate Medical Education (ACGME)−accredited residency (physical medicine and rehabilitation, or anesthesiology) or fellowship (sports medicine or pain medicine) programs from 2000 to 2015.PatientsAll patients receiving fluoroscopically guided hip (HI), sacroiliac joint (SIJI), transforaminal epidural (TFEI), and/or interlaminar epidural injections (ILEI, performed at only 1 institution).MethodsOutcome measures were examined based on the presence or absence of a trainee during the procedure.Main Outcome MeasurementsThe primary outcome was the number of immediate complications, with secondary outcomes being fluoroscopic time per injection (FTPI) and immediate numeric rating scale percentage improvement.ResultsTrainees were involved in 67.0% of all injections (N = 7,833). Complication rates or improvements in numeric rating scale scores showed no significant differences with trainee involvement for any injection type (P > .05). Trainee involvement was associated with increased FTPI for ILEIs (18.2 ± 10.1 seconds with trainees versus 15.1 ± 8.5 seconds without trainees, P < .001), but not for HIs (P = .60) or SIJIs (P = .51). Trainee involvement with TFEIs was dependent on institution for outcome with respect to FTPI (P < .001), with 28.1 ± 17.9 seconds with trainees and 32.1 ± 22.1 seconds without trainees (P = 0.51).ConclusionsThis large multicenter study of academic institutions demonstrates that trainee involvement in fluoroscopically guided injections does not affect immediate patient complications or pain improvement. Trainee involvement does not increase fluoroscopic time for most injections, although there is an institutional difference seen. This study supports the notion that appropriate trainee supervision likely does not compromise patient safety for fluoroscopically guided injections.Level of EvidenceII
Robustness analysis on aerial deployment motion of a Mars aircraft using multibody dynamics simulation: effects of wing-unfolding torque and timing
Authors: Fujita Koji , Nagai Hiroki 
Published: January 2017

ABSTRACTThis paper investigates the effects of the design variables of an aerial deployment mechanism on the robustness of the aerial deployment through a multibody dynamics simulation. The aircraft is modelled as three joined rigid bodies: a right wing, a left wing and a centre body. A spring-loaded hinge is adopted as an actuator for deployment. The design variables are the hinge torque and the deployment timing. The robustness is evaluated using a sigma level method. The margins for the safe deployment conditions are set for the evaluation functions. The dispersive input variables are the initial drop velocity, the surrounding gust velocity, the initial pitch angle and the initial height. The design point with a deployment torque scale valueFof 0.7 and a right-wing deployment delay timeTSRof 1.0 s can safely deploy in the low-torque deployment condition. This design point is able to accomplish both a safe deployment and a lightweight deployment mechanism.
Hegel and Husserl on the Emergence of the I out of Subjectivity
Authors: Ferrarin Alfredo 
Published: January 2017

AbstractModern philosophy tends to conflate subjectivity and ego (I-think, cogito, and the like). One lesson we can draw from Hegel is that the I emerges out of a natural and habitual state in the form of a return to itself through an opposition between self and world. In turn, Husserl has an interesting take on the anonymity of an ego-less subjectivity submerged in an affective and initially passive life out of which an ego-pole first constitutes itself. In both, a latent, functioning subjectivity which forms an unconscious ground is to be kept distinct from the several activities of a wakeful and self-conscious mind. I wish to compare and contrast Hegel and Husserl on this theme. The primary texts for my examination will be Hegel’s Phenomenology of Spirit and the Philosophy of Subjective Spirit in the Encyclopaedia, and Husserl’s Ideas I, Ideas II, Cartesian Meditations and Experience and Judgment.
Glacier changes on the Tibetan Plateau derived from Landsat imagery: mid-1970s – 2000–13
Authors: YE QINGHUA , ZONG JIBIAO , TIAN LIDE , COGLEY J. GRAHAM , SONG CHUNQIAO , GUO WANQIN 
Published: January 2017

ABSTRACTGlacier area changes on the Tibetan Plateau were studied in different drainage basins based on Landsat satellite images from three epochs: 263 in the mid-1970s, 150 in 1999–2002 and 148 in 2013/14. Three mosaics (M1976, M2001 and M2013) with minimal cloud and snow cover were constructed, and the uncertainty due to each epoch having a finite span was accounted for. Glacier outlines (TPG1976, TPG2001 and TPG2013) were digitized manually with guidance from the SRTM DEM v4.1 and Google Earth imagery. To achieve complete multi-temporal coverage in a reasonable time, only debris-free ice was delineated. Area mapping uncertainty was evaluated at three study sites, Mount Qomolangma (Everest), Mount Naimona'Nyi, Mount Geladandong, where the largest differences between present and earlier measurements were within ~±4%. Area differences with previous inventories ranged from −19.6% (TPG1976 minus the first Chinese Glacier Inventory) to −3.6% and −1.1% (TPG2013 and TPG2001, respectively minus the second Chinese Glacier Inventory), while the difference TPG2001 minus the GAMDAM Glacier Inventory was +10.4%. Glacier area on the plateau decreased from 44 366 ± 2827 km2(1.7% of the study area) in the 1970s to 42 210 ± 1621 km2in 2001 and 41 137 ± 1616 km2in 2013. Shrinkage was faster in external drainage basins of the southeast than in the interior basins of the northwest, from a maximum of −0.43% a−1(−1.60% a−1during 1994–2013) in the Mekong catchment down to a minimum of −0.12% a−1in the Tarim interior drainage.
Restricting the marketing of foods and non-alcoholic beverages to children in South Africa: are all nutrient profiling models the same?
Authors: Wicks Mariaan , Wright Hattie , Wentzel-Viljoen Edelweiss 
Published: January 2017

AbstractThe WHO has called for governments to improve children’s food environment by implementing restrictions on the marketing of ‘unhealthy’ foods to children. Nutrient profiling (NP) models are used to define ‘unhealthy’ foods and support child-directed food marketing regulations. The aim of the present study was to assess the suitability of the South African NP model (SANPM), developed and validated for health claim regulations, for child-directed food marketing regulations. The SANPM was compared with four NP models specifically developed for such regulations. A representative list of 197 foods was compiled by including all foods advertised on South African free-to-air television channels in 2014 and foods commonly consumed by South African children. The nutritional information of the foods was sourced from food packaging, company websites and a food composition table. Each individual food was classified by each of the five NP models. The percentage of foods that would be allowed according to the different NP models ranged from 6 to 45 %; the models also varied considerably with regard to the type of foods allowed for marketing to children. The majority of the pairwise comparisons between the NP models yieldedκstatistics >0·4, indicating a moderate agreement between the models. An almost perfect pairwise agreement (κ=0·948) existed between the SANPM and the UK Food Standards Agency model (United Kingdom Office of Communication nutrient profiling model), a model extensively tested and validated for such regulations. The SANPM is considered appropriate for child-directed food marketing regulations in South Africa.
Collagen peptide ingestion alters lipid metabolism-related gene expression and the unfolded protein response in mouse liver
Authors: Tometsuka Chisa , Koyama Yoh-ichi , Ishijima Tomoko , Toyoda Tsudoi , Teranishi Miki , Takehana Kazushige , Abe Keiko , Nakai Yuji 
Published: January 2017

AbstractIngestion of collagen peptide (CP) elicits beneficial effects on the body, including improvement in blood lipid profiles, but the underlying mechanisms remain unclear. The purpose of this study was to investigate the effects of CP ingestion on the liver, which controls lipid metabolism in the body. Male BALB/cCrSlc mice were bred with the AIN-93M diet containing 14 % casein or the AIN-93M-based low-protein diet containing 10 % casein or a diet containing 6 % casein+4 % CP for 10 weeks (n12/group). Total, free and esterified cholesterol levels in the blood decreased in the CP group. DNA microarray analysis of the liver revealed that expressions of genes related to lipid metabolic processes such as the PPAR signalling pathway and fatty acid metabolism increased in the CP group compared with the 10 % casein group. The expressions of several genes involved in steroid metabolic process, includingCyp7a1andCyp8b1, were decreased, despite being targets of transcriptional regulation by PPAR. These data suggest that lipid metabolism in the liver is altered by CP ingestion, and the decrease in blood cholesterol levels in the CP group is not due to enhancement of the steroid metabolic process. On the other hand, expressions of genes related to the unfolded protein response (UPR) significantly decreased at the mRNA level, suggesting that CP ingestion lowers endoplasmic reticulum stress. Indeed, protein levels of phosphorylated inositol-requiring enzyme 1 decreased after CP ingestion. Taken together, CP affects the broader pathways in the liver – not only lipid metabolism but also UPR.
Supplementing the maternal diet of rats with butyrate enhances mitochondrial biogenesis in the skeletal muscles of weaned offspring
Authors: Huang Yanping , Gao Shixing , Jun Guo , Zhao Ruqian , Yang Xiaojing 
Published: January 2017

AbstractThe present study aimed to investigate the effects of maternal dietary butyrate supplementation on energy metabolism and mitochondrial biogenesis in offspring skeletal muscle and the possible mediating mechanisms. Virgin female rats were randomly assigned to either control or butyrate diets (1 % butyrate sodium) throughout gestation and lactation. At the end of lactation (21 d), the offspring were killed by exsanguination from the abdominal aorta under anaesthesia. The results showed that maternal butyrate supplementation throughout gestation and lactation did not affect offspring body weight. However, the protein expressions of G-protein-coupled receptors (GPR) 43 and 41 were significantly enhanced in offspring skeletal muscle of the maternal butyrate-supplemented group. The ATP content, most of mitochondrial DNA-encoded gene expressions, the cytochrome c oxidase subunit 1 and 4 protein contents and the mitochondrial DNA copy number were significantly higher in the butyrate group than in the control group. Meanwhile, the protein expressions of type 1 myosin heavy chain, mitochondrial transcription factor A, PPAR-coactivator-1α (PGC-1α) and uncoupling protein 3 were significantly increased in the gastrocnemius muscle of the treatment group compared with the control group. These results indicate for the first time that maternal butyrate supplementation during the gestation and lactation periods influenced energy metabolism and mitochondrial biogenesis through the GPR and PGC-1α pathways in offspring skeletal muscle at weaning.