bluuebunny/crossref_metadata_2025_split huggingface dataset:
HIGH DENSITY JUVENILE SHRIMP (Penaeus monodon) PRODUCTION WITH DIFFERENT DURATIONS OF REARING IN ACID SULFATE SOIL-AFFECTED POND
Authors: Mustafa Akhmad , Sammut Jesmond 
Published: October 2016

An experiment was conducted to evaluate the effectiveness of producing juvenile shrimp in a reservoir pond constructed in acid sulphate soils (ASS). Many ponds constructed in ASS pose a significant economic risk due to high mortality and poor growth. In Indonesia, abandonment of ponds in ASS is widespread and alternative farming practices, such as production of juvenile shrimp, offer farms opportunities to lower their production risks. The experiment involved rearing juvenile shrimp at a high stocking density (3,000 individuals/m2) using hapas in a formerly unproductive pond located in Luwu Regency, South Sulawesi, Indonesia. The treatments tested included rearing periods of three and six weeks with four replications. A survival of 83.62% was achieved after rearing for three weeks compared to 45.44% after six weeks. Although there was a sixfold increase in the mean weight of shrimp from three to six weeks of culture, size variation after six weeks was large due to the high density of shrimp. The experiment showed that old acid sulphate soil-affected ponds, in which the bottom soil has not been excavated any further, can be used for production of juvenile shrimp in hapas. Rearing over three weeks is recommended to obtain high survival and reduce size variation.
INFLUENCE OF FEEDING TIME ON FEED UTILIZATION BY SIAMESE CATFISH, Pangasius hypophthalmus JUVENILE
Authors: Meilisza Nina , Moreau Yann , Baras Ettiene , Hirnawati Rina 
Published: October 2016

Siamese catfish (Pangasius hypophthalmus) at juvenile stage is used as ornamental fish by fish hobbies because of their beautiful shape and movement. Feeding time is a part of feeding management to get the best and effective strategy on feed input. This study aimed to determine the growth of Siamese catfish juvenile by looking at the influence of feeding time on feed utilization. The average individual weight of juveniles used was ± 3.2 g stocked 15 fish per aquarium equipped with recirculation system. Two level factors in the factorial design consisted of 4 feeding times (at 8:00, at 12:00, at 16:00, and as control (8:00, 12:00, 16:00) and feeding level (45 g feed kg-1 fish, 90 g feed kg-1 fish). Interaction between feeding time and feeding level resulting eight types of treatments research, namely A (8/45), B (12/45), C (16/45), D (8:12:16/45), E (8:12:16/90), F (8/90), G (12/90), and H (16/90). The results showed that feeding time had significant effect on the specific growth rate, feed conversion, and protein efficiency ratio and were significantly different among the treatments (P<0.05). Besides feeding time control (treatments D and E), treatment C also showed the highest of specific growth rate (2.03%), protein efficiency ratio (1.46), and the lowest feed conversion ratio (1.85).
FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION
Authors: Laining Asda , Rachmansyah Rachmansyah , Lideman Lideman , Koshio Shunsuke 
Published: October 2016

In order to investigate the phytic acid degradation in the gut of post juvenile Japanese flounder, indirect method was carried out by measuring the pre-prandial and postprandial plasma mineral and alkaline phosphatase (ALP) level as well as liver phosphorus content. The experiment was designed into a Randomized Block in which experiment units were grouped according to sampling days at 10, 20 and 30 days of feeding time. Experimental diets contained three levels of dietary inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary phytase at 0 and 2,000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g) were randomly distributed into 6 tanks of a 200 L capacity with density of 15 fish/tank. Blood sampling was carried out at 0 hour (before feeding or pre-prandial) and at 1, 3, 6 and 12 hour post feeding (post-prandial) time in three sampling days, respectively. Plasma was measured for mineral and ALP levels, while liver was analyzed for P content. The observation showed that fish fed without both dietary IP and phytase supplements had the lowest postprandial plasma IP, Mg and ALP levels during 12-h postprandial period. Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were significant higher when diet supplemented with phytase than those without phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding, plasma Ca level increased in all groups, but significant difference was only observed between group fed diet without both dietary IP and phytase and other groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration occurred in fish fed 0.25% IP together with phytase, and did not significantly differ from fish fed with 0.5% IP even when phytase was included in diet.
MOLECULAR IDENTIFICATION AND CLONAL RELATION OF ATYPICAL ISOLATE Aeromonas salmonicida USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)
Authors: Priyatna Riza , Indaryulianto Indaryulianto , Kurniasih Kurniasih 
Published: October 2016

Aeromonas salmonicida is responsible in many cases of furunculosis outbreaks resulting in economic loss of freshwater aquaculture. Four isolates of A. salmonicida have been isolated from fish collected in four different regions in Indonesia and its clonal relation has yet to be determined. In the research, A. salmonicida isolates and ATCC atypical isolate as the control had been checked for their clonal relation using Restriction Fragment Length Polymorphism (RFLP) method in which restriction enzyme of AluI, HaeIII, MboI, and EheI were used. PCR test results using the primers 16S rDNA amplicon gave a positive response to the 1300 bp band. The result of RFLP analysis showed that A. salmonicida atypical isolates from Indonesia represent subspecies smithia except isolates from C. macropomum in Yogyakarta in MS and 16S rDNA regions. Isolates from Jambi, Pontianak and Semarang showed a slight variation on enzyme restriction sites. Isolates number 2, 3, 4 and 5 had the same restriction sites using AluI enzyme with MS primer. The restriction enzymes that could give the best result for RFLP method of A. salmonicida were HaeIII, MboI and EheI.
THE USE OF REMOTE SENSING, REGRESSION QUANTILES, AND GIS APPROACHES FOR MODELING OF SCALLOP LARVAE: A Case Study in Funka Bay, Hokkaido, Japan
Authors: Radiarta I Nyoman 
Published: October 2016

In the development of scallop cultivation in Japan, larvae collection and propagation become an important factor. Although the monitoring program has been conducted, modeling of species distribution is becoming an important tool for understanding the effects of environmental changes and resources management. This study was conducted to construct a model for providing estimation of the scallop larvae distribution in Funka Bay, Hokkaido, Japan using the integration of remote sensing, Regression Quantile (RQ) and Geographic Information System (GIS)-based model. Data on scallop larvae were collected during one year spawning season from April to July 2003. Environmental parameters were extracted from multi sensor remotely sensed data (chlorophyll-a and sea surface temperature) and a hydrographic chart (water depth). These parameters together with larvae data were then analyzed using RQ. Finally, spatial models were constructed within a GIS by combining the RQ models with digital map of environmental parameters. The results show that the model was best explained by using only sea surface temperature. The highest larvae densities were predicted in a relatively broad distribution along with the shallow water regions (Toyoura and Sawara to Yakumo) and the deeper water areas (center of the bay). The spatial model built from the RQ provided robust estimation of the scallop larvae distributions in the study area, as confirmed by model validation using independent data. These findings could contribute on the monitoring program in this region in order to distinguish the potential areas for an effective spat collection.
DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER
Authors: Marnis Huria , Dewi Raden Roro Sri Pudji Sinarni , Imron Imron , Iswanto Bambang 
Published: October 2016

Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F0). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P<0.05).
THE DYNAMIC OF LANDSCAPE CHANGE AT COAST AREA, IN LABAKKANG SUBDISTRICT PANGKEP REGENCY, SOUTH SULAWESI
Authors: Tantu Andi Gusti , Soemarno Soemarno , Harahab Nuddin , Mustafa Akhmad 
Published: October 2016

Development is a changing process to improve human standard of life that strongly related to the activities of natural resource utilization. It is frequently found that these activities change the ecosystems and their resources. Eventually, these changes wil l give ser ious impacts to the envi ronments. The most distinct environmental problems are caused by resident migration to the coastal areas, coastal developments, and land limitation. Coastal reclamation is an example of human effort to respond to the land limitation in the coastal areas as observed in coastal areas of Labakkang Subdistrict. Reclamation activity is doing along the coast of Pundata Baji Village, whereas, in other villages, cutting off of mangrove areas as an alternative land expansion is increasingly practiced by the local communities. This Research was aimed to observe the landscape change from 1980 to 2010 at coast area in Labakkang Subdistrict. Each review considers Landsat Image Map (acquired in 1980); Landsat Image Map (acquired in 1990); Landsat Image Map-7 Enhanced Thematic Mapper Plus (ETM+) acquired in 2000); Spot Image 4 (acquired in 2005); and Spot 4 LAPAN (acquired in 2010). Result of research indicates that at coast area of Labakkang Subdistrict in 1980, there are 248.3 ha of mangrove vegetation, 2,756.63 ha inundation, and 4,157.0 ha open land. In 1990, there are 234.2 ha mangrove vegetation, 2,251.63 ha embankment, 933.2 ha rice field, and 582.0 ha open land. In 2000, there are 218.3 ha mangrove vegetation, 2,848.1 ha embankment, and 3,579.2 ha rice field. In 2005, it has 121.4 ha mangrove vegetation, 3,762.6 ha embankment, and 2,306.2 ha rice field. In 2010, it is found 48.9 ha mangrove vegetation, 5,029.35 ha embankment, and 749.98 ha rice field.
DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR
Authors: Fahmi Melta Rini , Pouyaud Laurent , Berrebi Patrick 
Published: October 2016

Tropical eels living in Indonesian waters are known to be composed of several species, but their real listing together with their distribution ranges need to be established. The main difficulties are the very high number of islands with perennial rivers where these species are living during the growth phase of their life cycle. It is difficult, sometimes impossible, to determine the species using morphological characters, moreover on glass eels. In order to establish the geographic distribution of tropical eels of the genus Anguilla in Indonesian waters, a total 1,115 specimens were collected between 2008 and 2012. Sample collection was done in the growth habitats that are rivers and estuaries by commercial nets of different categories according to the fish size. All samples were identified genetically using the recently developed semimultiplex PCR method. We recognized four species and subspecies with wide distribution: Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla marmorata, and Anguilla interioris; two species with limited distribution, close to endemism: Anguilla celebesensis and Anguilla borneensis and one subspecies Anguilla nebulosa nebulosa that is only spread in river flowing into Indian Ocean.
THE IMPACTS OF FASTING PERIODS ON FOOD INTAKE, GROWTH RATE, COMPENSATORY GROWTH, AND EFFICIENCY OF FEED UTILIZATION IN BLUE TILAPIA (Oreochromis aureus) REARED IN BRACKISH WATER PONDS
Authors: Setyawan Priadi , Robisalmi Adam , Listiyowati Nunuk , Dewi Raden Roro Sri Pudji Sinarni , Imron Imron 
Published: October 2016

Blue tilapia (Oreochromis aureus) has known as euryhaline species refers to wide range of salinity tolerance. The pure population or hybrid with other strain of tilapia can be a good candidate in marginal coastal land use. Tilapia is the most important aquaculture species in Indonesia. Refers to FAO 2010, Indonesia is the third biggest of tilapia production after China and Egypt. One of the main problems in aquaculture is their feed as the major cost in fish farming. Various techniques had carried out to reduce of feed cost such as improving fish quality, sex reversal and feed management. This research aimed to determine of compensatory growth in tilapia as one technique in feed management. Fry obtained from natural spawning in freshwater pond. Acclimatization in 20 ppt made in aquarium for 4 days after one month reared in hapas. Fish reared in 2 m x 1 m hapas with five treatments and three replications. This research had conducted at brackish water pond in Yogyakarta. The treatments is A: one day fasting and six days feeding (1/6), B: 2/5, C: 3/4, D: 4/3 and E is control. Results showed that the biggest of average weight gain is treatment of E (68.36 g) followed by A (66.38 g), B (62.44 g), C (43.56 g), and D (27.30 g) respectively. One-way ANOVAs analysis with 95% of interval confidences continued with Tukey’s Pairwaise comparison showed that nothing significant different between E, A, and B. The biggest of daily growth rate and specific growth rate is E (0.75 g/day and 4.68 %bw/day) followed by A (0.73 g/day and 4.65 %bw/day); B (0.69 g/day and 4.58 %bw/day); C (0.48 g/day and 4.18% bw/day) and D (0.29 g/day and 3.65% bw/day) respectively. Survival rate of A is 73.00% followed by E. 72.00%, C. 71.00%, D. 69.69%, and B. 67.00% respectively. Feed conversion ratio of D is 0.83 followed by C (0.87), B (0.98), A (1.16), and E (1.41). Food efficiency ratio of D is 127.06% followed by C (118.75%), B (106.09%), A (88.87%), and E (73.38%). These results indicate that fasting of one day and two days has no significant effect on fish growth. Treatment A and B is the better treatment for fish culture refers to the better value of FCR, FER, and total weight gain.
INVESTIGATION ON BIODIVERSITY AND CONSERVATION OF FRESHWATER FISHES IN INDONESIA
Authors: Sudarto Sudarto , Paradis Emmanuel 
Published: October 2016

Indonesia is one of the richest regions in the world in terms of biodiversity. However, recent evidence has shown that tropical forests destruction has maintained at a high rate over the last few years in this country. At the same time, living resources in Indonesian freshwater ecosystems are important: this country ranks at number seven in terms of production of inland capture fisheries with 323,150 tonnes in 2008. Freshwater fishes represent 42% of the total estimated ichthyofauna, concentrated in 0.01% of the total water covered environment. This environment is closer to human activities, making it critically vulnerable to adverse impacts. Furthermore, there has been some recent debates on the general sustainability of fisheries and aquaculture production systems. This research was focused on initiating a multi-scale study of fish biodiversity in freshwater environments. Two specific actions have been started. A review based on the analyses of the existing scientific literature and of databases on fish taxonomy and distributions. In parallel to this work, a global analysis of the distribution of fish diversity in Indonesia was undertaken. This work aims at identifying the major features of this resource as well as its current and future threats.
EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE
Authors: Parenrengi Andi , Tenriulo Andi , Lante Samuel 
Published: October 2016

The role of tiger shrimp defense against invading pathogen on molecular level such antiviral gene expression is limited to be reported. Gene expression is a process which codes information of genes that is converted to the protein as a phenotype. Distribution of PmAV antivirus gene, that has been reported as an important gene on non-specific response immune, is needed to be observed to several organs/tissues and size of tiger shrimp. The aim of this study is to determine the distribution of gene antiviral expression at several organ/tissue and size of shrimp. The organs/tissues observed in this study were: gill, hepatopancres, muscle tissue, eyes, heart, stomach, gonad, and intestine. While the size of shrimp consisted of three groups, those are: (A) 10-20 g/ind., (B) 30-40 g/ind., and (C) 60-70 g/ind. Analysis of antiviral gene expression was performed by RNA extraction, followed by the cDNA syntesis, and amplification of gene expression by semi-quantitative PCR. The result of PCR optimation showed the optimal concentration of cDNA and primer was 1 μL and 50 mol, respectively for PCR final volume of 25 μL. Antiviral gene was expressed on the hepatopancreas and stomach in percentage of 50.0% and 16.7%, respectively. While the highest percentage of individual expressing the antiviral gene was observed in the shrimp size of C (66.7%), followed by B (50.0%) and A (16.7%). The result of study implied that the hepatopancreas has importantly involed in tiger shrimp defense mechanism on viral infection.
SPATIAL DISTRIBUTION OF SOME CHEMICAL CHARACTERISTICS OF AN ACID SULFATE SOIL-AFFECTED BRACKISHWATER PONDS IN THE COASTAL AREA OF LUWU REGENCY SOUTH SULAWESI PROVINCE
Authors: Ratnawati Erna , Mustafa Akhmad 
Published: October 2016

Spatial distribution of brackishwater pond soil has important role in the system of bioenvironment including brackishwater pond environment. This research was aimed to determine the spatial distribution of some chemical characteristics of an acid sulfate soil-affected brackishwater pond in coastal area of Luwu Regency South Sulawesi Province, Indonesia. ALOS AVNIR-2 images (acquisition 16 May 2008, 16 October 2008, 17 September 2009) were integrated with Indonesian Topographic maps to obtain base map. Sampling soil points were determined by simple random sampling in 104 points for two different soil depths i.e. 0-0.2 m and 0.5-0.7 m. A total of 18 soil chemical characteristics was measured in the field and analyzed in the laboratory. Geostatistic using kriging method in the ArcGIS 9.3 software was used to interpolate the data. The results of this study indicated that in general pond soil chemical characteristics in Luwu Regency could be categorized with high variability or relatively heterogenic wi th the value of var iation coefficient more than 36%. The soil characteristics that explain acidity had shown similar pattern in spatial distribution as well as other soil characteristics with soil nutrient. The high value of pH and low value of PO4 were generally found in the northern part of Luwu Regency, including East Lamasi, East Walenrang, Bua, and Ponrang Subdistricts. It is recommended that soil management in brackishwater ponds of Luwu Regency could be based on soil chemical characteristics so its could improve the production through minimizing the input, increasing carrying capacity, and avoiding environmental degradation.
FINE STRUCTURAL ANALYSIS OF UNPRODUCTIVE AND LOWPRODUCTIVE CELLS RESULTING ON CULTURED RSIV (IN VITRO)
Authors: Mahardika Ketut , Miyazaki Teruo 
Published: October 2016

Red seabream iridovirus (RSIV) has been known to induce enlarged cells and inclusion body bearing cells (IBCs) allowing virus particles to propagate within viral assembly site (VAS) in the cell cytoplasm. The aim of this study was to evaluate fine structural analysis of unproductive and low-productive cells resulting on RSIV-infected cultured grunt fin (GF) cells. GF cells were treated with semi purified RSIV, and incubated for 6-14 days post cultured. The cellular enlargement were harvested, processed, and analysis under electron microscopy. Electron microscopy revealed four types of cells that were productive enlarged cells and productive IBCs which were allowing propagation of virus particles within its cytoplasm, and unproductive enlarged cells and IBCs without virus particles. Most of them were unproductive enlarged cells (80,71%-98,20%). Unproductive enlarged cell had a nucleus with enlarged cytoplasm without production of VAS with virus propagation. While, unproductive IBC had an inclusion body that was delimited from the host-cell cytoplasm by a thin membranous boundary, and was developed without virus propagation. On the other hand, lowproductive enlarged cells and IBCs contained a few number of virus particles or tubule-like structures. Therefore, the number of low-productive enlarged cells and IBCs were only a few (about 0%-14% from a total of percent productive enlarged cells and IBCs), these cells were classified into types of productive enlarged cells and IBCs. These results sugested that the unproductive and low-productive enlarged cells and IBCs were the results of RSIV-infected GF cells which failed to produce virus particles due to incapacity of RSIV virus it self and or the ability of GF cells to inhibit virus multiplication within VAS.
FLUCTUATING ASYMMETRY REFLECT THE GROWTH OF HYBRID GROUPER Epinephelus fuscoguttatus AND Epinephelus polyphekadion
Authors: Sugama Ketut , Muzaki Ahmad , Permana I Gusti Ngurah , Haryanti Haryanti 
Published: October 2016

Fluctuating asymmetry has been widely used as a measure of developmental stability and as an indicator of individual fish growth. The present study compared fluctuating asymmetry in three bilateral meristic traits of F-1 hybrid between female Epinephelus fuscoguttatus and male Epinephelus polyphekadion and two F-1 pure parental progenies. The fishes were reared by communal and separate tank systems. Hybrids were confirmed by allozymes electrophoresis. After three months of rearing, the F-1 hybrids fish grew faster 45.9% and 66.6% compare to the F-1 pure parental progenies of E. fuscoguttatus and E. polyphekadion (P<005) respectively. Development stability as measured by fluctuating asymmetry was lower in hybrid (20%-40%) than in the pure parental progenies (53%-80%). Among four enzyme loci examined, only Pgm locus showed variants and at that locus all hybrids fish were heterozygous. Heterozygous hybrids showed lower fluctuating asymmetry and related to higher developmental stability. The higher developmental stability positively reflected a faster growth rate. Thus this finding can be used to explain the reason that why hybrids groupers appeared grew faster than parental species.