bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of non-invasive ventilation treatment practice for patients with chronic obstructive pulmonary disease:Results from a participatory research project
Authors: Christensen Helle M , Titlestad Ingrid L , Huniche Lotte 
Published: November 2017

Objectives: Non-invasive ventilation treatment for patients with acute exacerbation of chronic obstructive pulmonary disease is well documented. Communication with patients during treatment is inhibited because of the mask, the noise from the machine and patient distress. Assessing life expectancy and identifying end-stage chronic obstructive pulmonary disease posed difficulties and caused doubts concerning initiation and continuation of non-invasive ventilation as life-sustaining treatment. Health professionals expressed a need for knowledge of patients’ perspectives and attitude towards non-invasive ventilation. Methods: The study adheres to principles of Critical psychological practice research. Data on patients’ and health professionals’ perspectives were obtained from observations from the ward and semi-structured interviews with 16 patients. A group of health professionals was set up to form a co-researcher group. The co-researcher group described and analysed treatment practice at the department, drawing on research literature, results from observations and patients’ interviews. Results: Interviews revealed that 15 patients evaluated treatment with non-invasive ventilation positively, although 13 had experienced fear and 14 discomfort during treatment. The co-researcher group described health professionals’ perspectives and analysed treatment practice based on data from patients’ perspectives developing new management strategies in clinical practice with non-invasive ventilation. Conclusion: The participatory approach enabled continuous and complementary development of knowledge and treatment practice. The investigation of patient perspectives was particularly productive in qualifying cooperation among health professionals. The study resulted in preparing, and implementing, new clinical strategies.
Crosstalk between ROR1 and BCR pathways defines novel treatment strategies in mantle cell lymphoma
Authors: Karvonen Hanna , Chiron David , Niininen Wilhelmiina , Ek Sara , Jerkeman Mats , Moradi Elaheh , Nykter Matti , Heckman Caroline A. , Kallioniemi Olli , Murumägi Astrid , Ungureanu Daniela 
Published: November 2017

Key PointsTargeting ROR1 downregulates NF-κB p65 expression and sensitizes MCL cells to BCR- or Bcl-2–targeted drugs. Inhibition of BCR signaling by BTK-specific inhibitors such as ibrutinib impairs ROR1 levels and consecutively ROR1-targeted therapies.
Primary ciliary dyskinesia ciliated airway cells show increased susceptibility toHaemophilus influenzaebiofilm formation
Authors: Walker Woolf T. , Jackson Claire L. , Allan Raymond N. , Collins Samuel A. , Kelso Michael J. , Rineh Ardeshir , Yepuri Nageshwar R. , Nicholas Ben , Lau Laurie , Johnston David , Lackie Peter , Faust Saul N. , Lucas Jane S.A. , Hall-Stoodley Luanne 
Published: November 2017

Non-typeableHaemophilus influenzae(NTHi) is the most common pathogen in primary ciliary dyskinesia (PCD) patients. We hypothesised that abnormal ciliary motility and low airway nitric oxide (NO) levels on airway epithelial cells from PCD patients might be permissive for NTHi colonisation and biofilm development.We used a primary epithelial cell co-culture model to investigate NTHi infection. Primary airway epithelial cells from PCD and non-PCD patients were differentiated to ciliation using an air–liquid interface culture and then co-cultured with NTHi.NTHi adherence was greater on PCD epithelial cells compared to non-PCD cells (p<0.05) and the distribution of NTHi on PCD epithelium showed more aggregated NTHi in biofilms (p<0.001). Apart from defective ciliary motility, PCD cells did not significantly differ from non-PCD epithelial cells in the degree of ciliation and epithelial integrity or in cytokine, LL-37 and NO production. Treatment of PCD epithelia using exogenous NO and antibiotic significantly reduced NTHi viability in biofilms compared with antibiotic treatment alone.Impaired ciliary function was the primary defect in PCD airway epithelium underlying susceptibility to NTHi biofilm development compared with non-PCD epithelium. Although NO responses were similar, use of targeted NO with antibiotics enhanced killing of NTHi in biofilms, suggesting a novel therapeutic approach.
Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
Authors: Nursyirwani Nursyirwani , Asmara Widya , Wahyuni Agnesia Endang Tri Hastuti , Triyanto Triyanto , Fauzi Muhammad , Muchlisin Zainal Abidin 
Published: November 2017

Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10oC and 45oC, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae.
Multiplex Gene Expression Profiling of In Vivo Isolated Circulating Tumor Cells in High-Risk Prostate Cancer Patients
Authors: Markou Athina , Lazaridou Marifili , Paraskevopoulos Panagiotis , Chen Shukun , Świerczewska Monika , Budna Joanna , Kuske Andra , Gorges Tobias M , Joosse Simon A , Kroneis Thomas , Zabel Maciej , Sedlmayr Peter , Alix-Panabières Catherine , Pantel Klaus , Lianidou Evi S 
Published: November 2017

Abstract

BACKGROUND
Molecular characterization of circulating tumor cells (CTCs) is important for selecting patients for targeted treatments. We present, for the first time, results on gene expression profiling of CTCs isolated in vivo from high-risk prostate cancer (PCa) patients compared with CTC detected by 3 protein-based assays—CellSearch®, PSA-EPISPOT, and immunofluorescence of CellCollector® in vivo-captured CTCs—using the same blood draw.

METHODS
EpCAM-positive CTCs were isolated in vivo using the CellCollector from 108 high-risk PCa patients and 36 healthy volunteers. For 27 patients, samples were available before and after treatment. We developed highly sensitive multiplex RT-qPCR assays for 14 genes (KRT19, EpCAM, CDH1, HMBS, PSCA, ALDH1A1, PROM1, HPRT1, TWIST1, VIM, CDH2, B2M, PLS3, and PSA), including epithelial markers, stem cell markers, and epithelial-to-mesenchymal-transition (EMT) markers.

RESULTS
We observed high heterogeneity in gene expression in the captured CTCs for each patient. At least 1 marker was detected in 74 of 105 patients (70.5%), 2 markers in 45 of 105 (40.9%), and 3 markers in 16 of 105 (15.2%). Epithelial markers were detected in 31 of 105 (29.5%) patients, EMT markers in 46 of 105 (43.8%), and stem cell markers in 15 of 105 (14.3%) patients. EMT-marker positivity was very low before therapy (2 of 27, 7.4%), but it increased after therapy (17 of 27, 63.0%), whereas epithelial markers tended to decrease after therapy (2 of 27, 7.4%) compared with before therapy (13 of 27, 48.1%). At least 2 markers were expressed in 40.9% of patients, whereas the positivity was 19.6% for CellSearch, 38.1% for EPISPOT, and 43.8% for CellCollector-based IF-staining.

CONCLUSIONS
The combination of in vivo CTC isolation with downstream RNA analysis is highly promising as a high-throughput, specific, and ultrasensitive approach for multiplex liquid biopsy-based molecular diagnostics.
Mother of pearl, song and potatoes: Cultivating resilience in Claudia Llosa’s La teta asustada/Milk of Sorrow (2009)
Authors: Rojas Adriana 
Published: November 2017

Abstract
This article explores postmemory and the themes of transgenerational trauma and resilience in La teta asustada/The Milk of Sorrow by Claudia Llosa (2009), a film that suggests that the Peruvian internal conflict of the 1980s and the 1990s created a primal scene in collective memory, but departs from conventions of trauma narratives. Traumatized by the story of her mother’s rape, Fausta struggles to commemorate her dead mother’s experiences and bury her during a time meant for weddings. With help from her community, Fausta learns to cultivate resilience and develop personal agency. The use of song and the potato and pearl metaphors demonstrate how Fausta honours her mother’s memory and breaks from her mother’s narrative in order to formulate her own identity. Thus the film represents the cultural resilience of the postgeneration in renegotiating their identity after fleeing from mass violence.
Book Reviews
Authors: Lahr-Vivaz Elena , Leen Catherine , Pick Zuzana , Kearley Vicky , Toribio Nuria Triana , Cunha Mariana , Barreto Danny , Cerdán Josetxo 
Published: November 2017

Abstract
Screening Neoliberalism: Transforming Mexican Cinema 1988–2012, Ignacio Sánchez Prado (2014) Nashville: Vanderbilt University Press, 291 pp., ISBN: 9780826519658, h/bk, $79.95; ebook, $9.99
Mexican Screen Fiction: Between Cinema and Television, Paul Julian Smith (2014) Cambridge: Polity, 296 pp., ISBN: 0745680798, h/bk, $69.95; p/bk, £17.99/$24.95; eBook, $69.95
Revolution and Rebellion in Mexican Film, Niamh Thornton (2015) New York, London: Bloomsbury Academic, 213 pp., ISBN: 9781501305702, h/bk, $110, p/bk, £ 20, $39.66, e-book $34.99
Critical Approaches to the Films of Robert Rodriguez, Frederick Luis Aldama (ed.) (2015) Austin: University of Texas Press, 253 pp., ISBN: 9781477302408, p/bk-ebook, $24.95
Performance and Spanish Film, Dean Allbritton, Alejandro Melero and Tom Whittaker (eds) (2016) Manchester: Manchester University Press, 381 pp., ISBN: 9780719097720, h/bk, $110.00
Journeys in Argentine and Brazilian Cinema: Road Films in a Global Era, Natália Pinazza (2014) New York and London: Palgrave Macmillan, 187 pp., ISBN: 9781137336033, h/bk, $100; p/bk, $95.00; eBook, $69.99
New Maricón Cinema: Outing Latin American Film, Vinodh Venkatesh (2016) Austin: University of Texas Press, 252 pp., ISBN: 9781477310144, h/bk, $95
New Documentaries in Latin America, Vinicius Navarro and Juan Carlos Rodriguez (eds) (2014) New York: Palgrave Macmillan, 287 pp., ISBN: 9781137291332, h/bk, $100.00
Dietary weight loss‐induced changes in RBP4, FFA, and ACE predict weight regain in people with overweight and obesity
Authors: Vink Roel G. , Roumans Nadia J. , Mariman Edwin C. , van Baak Marleen A. 
Published: November 2017

AbstractAdipokines and other biomarkers were previously identified with roles in energy expenditure, appetite, satiety, and adiposity. Therefore, we investigated whether dietary weight loss‐induced changes in adipokines and other biomarkers known to play a role in weight regulation or energy expenditure could predict weight regain in people with overweight and obesity. In this randomized controlled trial 26 males and 30 females (BMI: 28–35 kg/m2) followed either a low‐calorie diet (LCD; 1250 kcal/day) for 12 weeks or a very‐low‐calorie diet (VLCD; 500 kcal/day) for 5 weeks followed by a weight stable period of 4 weeks (dietary intervention (DI) period) and a 9‐month follow‐up period. Blood samples were taken before and after each period to measure FFA, TAG, total cholesterol, insulin, glucose, angiotensin‐converting enzyme (ACE) activity, IL‐6, RBP4, apelin, leptin, adiponectin, vaspin, and nesfatin‐1 concentrations. Weight loss was similar between groups (LCD: −8.2 ± 0.5 kg; VLCD: −8.9 ± 0.4 kg, P = 0.30). Only changes in ACE activity, FFA and RBP4 concentrations during DI were correlated with weight regain in the whole group (r = −0.299, P = 0.030, r = −0.274, P = 0.047, and r = 0.357, P = 0.008, respectively). Together they explained 28% (r = 0.532) of weight regain variation. Dietary weight loss‐induced changes in ACE activity, FFA and RBP4 independently contribute to weight regain prediction.
DNPEP is not the only peptidase that produces SPAK fragments in kidney
Authors: Koumangoye Rainelli , Delpire Eric 
Published: November 2017

AbstractSPAK (STE20/SPS1‐related proline/alanine‐rich kinase) regulates Na+ and Cl− reabsorption in the distal convoluted tubule, and possibly in the thick ascending limb of Henle. This kinase phosphorylates and activates the apical Na‐Cl cotransporter in the DCT. Western blot analysis reveals that SPAK in kidney exists as a full‐length protein as well as shorter fragments that might affect NKCC2 function in the TAL. Recently, we showed that kidney lysates exerts proteolytic activity towards SPAK, resulting in the formation of multiple SPAK fragments with possible inhibitory effects on the kinase. The proteolytic activity is mediated by a Zn2+ metalloprotease inhibited by 1,10‐phenanthroline, DTT, and EDTA. Size exclusion chromatography demonstrated that the protease was a high‐molecular‐weight protein. Protein identification by mass‐spectrometry analysis after ion exchange and size exclusion chromatography identified multiple proteases as possible candidates and aspartyl aminopeptidase, DNPEP, shared all the properties of the kidney lysate activity. Furthermore, recombinant GST‐DNPEP produced similar proteolytic pattern. No mouse knockout model was, however, available to be used as negative control. In this study, we used a DNPEP‐mutant mouse generated by EUCOMM as well as a novel CRISPR/cas9 mouse knockout to assess the activity of their kidney lysates towards SPAK. Two mouse models had to be used because different anti‐DNPEP antibodies provided conflicting data on whether the EUCOMM mouse resulted in a true knockout. We show that in the absence of DNPEP, the kidney lysates retain their ability to cleave SPAK, indicating that DNPEP might have been misidentified as the protease behind the kidney lysate activity, or that the aspartyl aminopeptidase might not be the only protease cleaving SPAK in kidney.
Diminished dynamic cerebral autoregulatory capacity with forced oscillations in mean arterial pressure with elevated cardiorespiratory fitness
Authors: Labrecque Lawrence , Rahimaly Kevan , Imhoff Sarah , Paquette Myriam , Le Blanc Olivier , Malenfant Simon , Lucas Samuel J. E. , Bailey Damian M. , Smirl Jonathan D. , Brassard Patrice 
Published: November 2017

AbstractThe effect that cardiorespiratory fitness has on the dynamic cerebral autoregulatory capacity during changes in mean arterial pressure (MAP) remains equivocal. Using a multiple‐metrics approach, challenging MAP across the spectrum of physiological extremes (i.e., spontaneous through forced MAP oscillations), we characterized dynamic cerebral autoregulatory capacity in 19 male endurance athletes and eight controls via three methods: (1) onset of regulation (i.e., time delay before an increase in middle cerebral artery (MCA) conductance [MCA blood velocity (MCAv)/MAP] and rate of regulation, after transient hypotension induced by sit‐to‐stand, and transfer function analysis (TFA) of MAP and MCAv responses during (2) spontaneous and (3) forced oscillations (5‐min of squat‐stand maneuvers performed at 0.05 and 0.10 Hz). Reductions in MAP and mean MCAv (MCAVmean) during initial orthostatic stress (0‐30 sec after sit‐to‐stand) and the prevalence of orthostatic hypotension were also determined. Onset of regulation was delayed after sit‐to‐stand in athletes (3.1 ± 1.7 vs. 1.5 ± 1.0 sec; P = 0.03), but rate of regulation was not different between groups (0.24 ± 0.05 vs. 0.21 ± 0.09 sec−1; P = 0.82). While both groups had comparable TFA metrics during spontaneous oscillations, athletes had higher TFA gain during 0.10 Hz squat‐stand versus recreational controls (P = 0.01). Reductions in MAP (P = 0.15) and MCAVmean (P = 0.11) during orthostatic stress and the prevalence of initial orthostatic hypotension (P = 0.65) were comparable between groups. These results indicate an intact ability of the cerebral vasculature to react to spontaneous oscillations but an attenuated capability to counter rapid and large changes in MAP in individuals with elevated cardiorespiratory fitness.
Berlin 1936: The Creation of the “Myth” Jesse Owens
Authors: Leite Werlayne Stuart Soares 
Published: November 2017

Abstract
Throughout the twentieth century, the sport has gained much importance in society and sparked interest from various sectors, including the political. Adolf Hitler used the XI 1936 Summer Olympic Games to show the world the strength of Nazi Germany and its rebirth after the defeat in World War I and the impositions of the Versailles Treaty. However, many of the facts historically reported on the 1936 Olympics are contested. The most famous and mythical case of these Olympic Games, and one of the most famous in the history of sport, relates to events that occurred between the American athlete Jesse Owens and the Nazi Führer. The aim of this work is to try to show, as faithfully as possible, as some important facts occurred during this event (the contest between Owens and Long in the long jump; if Hitler snubbed Owens; etc.) that helped create the “myth” around Owens; and to present reports of the global media coverage, analyzing the perpetuation of these mythical reports in current media. As methodology was conducted an ample bibliographical research: reports taken from newspapers of the time and current, books, scientific papers, master's thesis, documentaries, etc. Without claiming to prove a single fact, it is intended to provide insight to the reader to draw their own conclusions.
Hybrid intervention scheme-based optimization algorithm for real-time management of reactive power resources
Authors: Dileep Deesh , Rueda Torres José Luis , Franke Sander , Palensky Peter 
Published: November 2017

Abstract
This article introduces a Hybrid Intervention Scheme Based Optimization (HIBO) algorithm solving an Optimal Reactive Power
Management (ORPM) problem in real-time using a Mixed Integer Linear Programming (MILP) solver. The ORPM problem presented
here contains a linear objective function containing four objectives separated using a set of static penalty factors for
each area. The non-linear optimization problem has been assumed linear by localizing the search for solution, this is done
by introducing a penalty on the change from the original state or the base case scenario. Thereby, optimizing the
non-linear ORPM in linear steps makes it a fast solver for small changes in power system state. A contingency analysis
(for N-1 voltage violations) is included for ensuring the safety and reliability of the power system. The results are used
to update the ORPM problem or stop if the system is secure. The optimization variables used to represent transformer taps
and shunt device switches are handled as discrete integers and remaining variables as continuous real numbers. The
intervention scheme, objectives and constraints used in the HIBO have been derived through surveys conducted at
a transmission system control center and are supported using literature. Validation of the HIBO algorithm was performed on
the Dutch transmission network model after dividing it into four regional areas. Convergence characteristics of the HIBO
algorithm are compared using results. From the results, it is concluded that the HIBO algorithm is a competitive
optimization solver, suitable for deployment in the secondary voltage control scheme within system operations domain for
transmission system operators.
Conductive switching behavior of epoxy resin/micron-aluminum particles composites
Authors: Lu Pin , Qu Zhaoming , Wang Qingguo , Wang Yan , Cheng Wei 
Published: November 2017

AbstractEpoxy resin (ER)/micron-aluminum particles (MP) composites with different filling concentration of spherical particles were fabricated. The nonlinear conductive behavior of ER/MP composites under increasing applied voltage using the improved V-I method was investigated. Under sufficient high intensity applied constant voltage, the obvious conductive switching behavior of samples with volume fraction 12.7%, 16.9% and 18.9% was found. The conductivity of ER/MP composites increase up to 3–4 orders of magnitude when the conductor-insulator transition occurs. The switching threshold voltage decreases with the increase of volume fraction of MP in the composites. The results show that the ER/MP composites with conductive switching properties are of great potential application in electromagnetic protection of electron devices and systems.
Patent activities of the Czech enterprises – an international comparison
Authors: Kučera Zdeněk , Vondrák Tomáš , Pecha Ondřej 
Published: November 2017

Abstract
The Czech enterprise sector shows in a long term a significantly lower patent activity in comparison with both the most innovation-intensive EU economies, and the EU-15 average. The topic of this contribution is a comparison of the quality and the volume of the industrial property rights created in the Czech enterprises with a selected EU-15 countries classified as innovation leaders, strong and moderate innovators. Two new EU members, namely Poland and Hungary, were also included in the comparisons. The analysis shows that the number of the international patent applications registered by enterprises with the EPO and under the PCT treaty is by more than one order lower in comparison with both the innovation-intensive countries like Denmark, Germany, or Netherlands and the countries whose innovation performance is comparable to the Czech Republic. The Czech enterprises unlike their counterparts in the innovation-intensive countries register the priority applications with the home country patent office and only a small fraction is subsequently registered with EPO and under the PCT. Also the size of the patent families and subsequent citations of the Czech patents by other patent applications lags behind the innovation-intensive countries.
The low patenting activity of the Czech enterprises correlates with a low expenditure on R&D. The Czech enterprises are somehow limited in the creation of knowledge giving rise to internationally competitive products which would demand international industrial property claims. The analysis also indicates that patent applications co-authored by Czech inventors are extensively owned by foreign corporations. The Czech enterprises produce a larger fraction of patents in collaboration with research organizations than foreign ones. It indicates that enterprises are interested in tapping in the research capacity of the universities and governmental R&D establishment. The detail analysis of the data in the national R&D Information System indicates that the number of patents created by enterprises supported by applied R&D programs is currently rather low. Beside the stimulation of the Czech enterprises to the in-house R&D spending it is necessary to support long-term strategic alliances of enterprises with research organizations. It will create a fertile environment for the development of radical innovations which are a key factor for the increase of international competitiveness and market shares.