bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of chronic cobalt and chromium exposure after metal‐on‐metal hip resurfacing: An epigenome‐wide association pilot study
Authors: Steinberg Julia , Shah Karan M. , Gartland Alison , Zeggini Eleftheria , Wilkinson Jeremy Mark 
Published: January 2017

ABSTRACTMetal‐on‐metal (MOM) hip resurfacing has recently been a popular prosthesis choice for the treatment of symptomatic arthritis, but results in the release of cobalt and chromium ions into the circulation that can be associated with adverse clinical effects. The mechanism underlying these effects remains unclear. While chromosomal aneuploidy and translocations are associated with this exposure, the presence of subtle structural epigenetic modifications in patients with MOM joint replacements remains unexplored. Consequently, we analyzed whole blood DNA methylation in 34 OA patients with MOM hip resurfacing (MOM HR) compared to 34 OA patients with non‐MOM total hip replacements (non‐MOM THR), using the genome‐wide Illumina HumanMethylation 450k BeadChip. No probes showed differential methylation significant at 5% false‐discovery rate (FDR). We also tested association of probe methylation levels with blood chromium and cobalt levels directly; there were no significant associations at 5% FDR. Finally, we used the “epigenetic clock” to compare estimated to actual age at sample for all individuals. We found no significant difference between MOM HR and non‐MOM THR, and no correlation of age acceleration with blood metal levels. Our results suggest the absence of large methylation differences systemically following metal exposure, however, larger sample sizes will be required to identify potential small effects. Any DNA methylation changes that may occur in the local periprosthetic tissues remain to be elucidated. © 2017 The Authors. Orthopaedic Research Society. Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 35:2323–2328, 2017.
Effect of sequential fermentations and grape cultivars on volatile compounds and sensory profiles of Danish wines
Authors: Liu Jing , Arneborg Nils , Toldam‐Andersen Torben B , Petersen Mikael A , Bredie Wender LP 
Published: January 2017

AbstractBACKGROUNDThere has been an increasing interest in the use of selected non‐Saccharomyces yeasts in co‐culture with Saccharomyces cerevisiae. In this work, three non‐Saccharomyces yeast strains (Metschnikowia viticola, Metschnikowia fructicola and Hanseniaspora uvarum) indigenously isolated in Denmark were used in sequential fermentations with S. cerevisiae on three cool‐climate grape cultivars, Bolero, Rondo and Regent. During the fermentations, the yeast growth was determined as well as key oenological parameters, volatile compounds and sensory properties of finished rosé wines.RESULTSThe different non‐Saccharomyces strains and cool‐climate grape cultivars produced wines with a distinctive aromatic profile. A total of 67 volatile compounds were identified, including 43 esters, 14 alcohols, five acids, two ketones, a C13‐norisoprenoid, a lactone and a sulfur compound. The use of M. viticola in sequential fermentation with S. cerevisiae resulted in richer berry and fruity flavours in wines. The sensory plot showed a more clear separation among wine samples by grape cultivars compared with yeast strains.CONCLUSIONKnowledge on the influence of indigenous non‐Saccharomyces strains and grape cultivars on the flavour generation contributed to producing diverse wines in cool‐climate wine regions. © 2017 Society of Chemical Industry
Sandhill crane roost selection, human disturbance, and forage resources
Authors: Pearse Aaron T. , Krapu Gary L. , Brandt David A. 
Published: January 2017

ABSTRACTSites used for roosting represent a key habitat requirement for many species of birds because availability and quality of roost sites can influence individual fitness. Birds select roost sites based on numerous factors, requirements, and motivations, and selection of roosts can be dynamic in time and space because of various ecological and environmental influences. For sandhill cranes (Antigone canadensis) at their main spring‐staging area along the Platte River in south‐central Nebraska, USA, past investigations of roosting cranes focused on physical channel characteristics related to perceived security as motivating roost distribution. We used 6,310 roost sites selected by 313 sandhill cranes over 5 spring migration seasons (2003–2007) to quantify resource selection functions of roost sites on the central Platte River using a discrete choice analysis. Sandhill cranes generally showed stronger selection for wider channels with shorter bank vegetation situated farther from potential human disturbance features such as roads, bridges, and dwellings. Furthermore, selection for roost sites with preferable physical characteristics (wide channels with short bank vegetation) was more resilient to nearby disturbance features than more narrow channels with taller bank vegetation. The amount of cornfields surrounding sandhill crane roost sites positively influenced relative probability of use but only for more narrow channels <100 m and those with shorter bank vegetation. We confirmed key resource features that sandhill cranes selected at river channels along the Platte River, and after incorporating spatial variation due to human disturbance, our understanding of roost site selection was more robust, providing insights on how disturbance may interact with physical habitat features. Managers can use information on roost‐site selection when developing plans to increase probability of crane use at existing roost sites and to identify new areas for potential use if existing sites become limited. © 2016 The Wildlife Society.
On the global existence and time decay estimates in critical spaces for the Navier–Stokes–Poisson system
Authors: Chikami Noboru , Danchin Raphaël 
Published: January 2017

AbstractWe are concerned with the study of the Cauchy problem for the Navier–Stokes–Poisson system in the critical regularity framework. In the case of a repulsive potential, we first establish the unique global solvability in any dimension for small perturbations of a linearly stable constant state. Next, under a suitable additional condition involving only the low frequencies of the data and in the L2‐critical framework (for simplicity), we exhibit optimal decay estimates for the constructed global solutions, which are similar to those of the barotropic compressible Navier–Stokes system. Our results rely on new a priori estimates for the linearized Navier–Stokes–Poisson system about a stable constant equilibrium, and on a refined time‐weighted energy functional.
Technical Note: Measurement of common carotid artery lumen dynamics using black‐blood MR cine imaging
Authors: Dai Erpeng , Dong Li , Zhang Zhe , Li Lyu , Zhang Hui , Zhao Xihai , Wang Jinnan , Yuan Chun , Guo Hua 
Published: January 2017

PurposeTo demonstrate the feasibility of measuring the common carotid artery (CCA) lumen dynamics using a black‐blood cine (BB‐cine) imaging method.MethodsMotion‐sensitized driven‐equilibrium (MSDE) prepared spoiled gradient sequence was used for the BB‐cine imaging. CCAs of eleven healthy volunteers were studied using this method. Lumen dynamics, including lumen area evolution waveforms and distension values, were measured and evaluated by comparing this method with bright‐blood cine (BrB‐cine) imaging.ResultsCompared with the BrB‐cine images, flow artifacts were effectively suppressed in the BB‐cine images. BrB‐cine images generally show larger lumen areas than BB‐cine images. The lumen area waveforms and distension measurements from BB‐cine imaging showed smaller variances among different subjects than BrB‐cine imaging.ConclusionsThe proposed BB‐cine imaging technique can suppress the flow artifacts effectively and reduce the partial volume effects from the vessel wall. This might allow more accurate lumen dynamics measurements than traditional BrB‐cine imaging, which may further be valuable for investigating biomechanical and functional properties of the cardiovascular system.
Positive contrast from cells labeled with iron oxide nanoparticles: Quantitation of imaging data
Authors: Magnitsky Sergey , Zhang Jinjin , Idiyatullin Djaudat , Mohan Geetha , Garwood Michael , Lane Nancy E. , Majumdar Sharmila 
Published: January 2017

PurposeConventional T2‐weighted MRI produces a hypointense signal from iron‐labeled cells, which renders quantification unfeasible. We tested a SWeep Imaging with Fourier Transformation (SWIFT) MRI pulse sequence to generate a quantifiable hyperintense signal from iron‐labeled cells.MethodsMesenchymal stem cells (MSCs) were labeled with different concentrations of iron oxide particles and examined for cell viability, proliferation, and differentiation. The SWIFT sequence was optimized to detect and quantify the amount of iron in the muscle tissue after injection of iron oxide solution and iron‐labeled MSCs.ResultsThe incubation of MSCs with iron oxide and low concentration of poly‐L‐lysine mixture resulted in an internalization of up to 22 pg of iron per cell with no adverse effect on MSCs. Phantom experiments showed a dependence of SWIFT signal intensity on the excitation flip angle. The hyperintense signal from iron‐labeled cells or solutions was detected, and an amount of the iron oxide in the tissue was quantified with the variable flip angle method.ConclusionsThe SWIFT sequence can produce a quantifiable hyperintense MRI signal from iron‐labeled cells. The graft of 18 x 106 cells was detectable for 19 days after injection and the amount of iron was quantifiable. The proposed protocol simplifies the detection and provides a means to quantify cell numbers. Magn Reson Med 78:1900–1910, 2017. © 2017 International Society for Magnetic Resonance in Medicine.
Photothermal Conversion of W18O49 with a Tunable Oxidation State
Authors: Fang Zhenxing , Jiao Shihui , Kang Yutang , Pang Guangsheng , Feng Shouhua 
Published: January 2017

AbstractW18O49 with a tunable oxidation state was prepared by addition of NaNO3 or NaBH4 as a redox agent in the solvothermal system. The addition of redox agents has no influence on the crystallization of W18O49. The obtained W18O49 structures keep their morphology as a bundle of nanowires with a regular hexagonal on the cross‐section. W18O49 exhibits strong valence‐dependent absorption features in the near‐IR region. Reduced W18O49 with more W5+ has a higher concentration of oxygen vacancies, which enhances the localized surface plasmon resonance effect. Reduced W18O49 exhibits a high photothermal conversion efficiency of 59.6 % and has good photothermal stability.
In Brief: Myeloid‐derived suppressor cells in cancer
Authors: Solito S , Pinton L , Mandruzzato S 
Published: January 2017

AbstractThe role of myeloid‐derived suppressor cells (MDSCs) in cancer development has become clear over recent years, and MDSC targeting is an emerging opportunity for enhancing the effectiveness of current anticancer therapies. As MDSCs are not only able to limit anti‐tumour T‐cell responses, but also to promote tumour angiogenesis and invasion, their monitoring has prognostic and predictive value. Herein, we review the key features of MDSCs in cancer promotion. © 2017 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
FISH identifies a KAT6A/CREBBP fusion caused by a cryptic insertional t(8;16) in a case of spontaneously remitting congenital acute myeloid leukemia with a normal karyotype
Authors: Barrett Rachel , Morash Barbara , Roback David , Pambrun Chantale , Marfleet Lesley , Ketterling Rhett P. , Harrison Karen , Berman Jason N. 
Published: January 2017

AbstractCytogenetics can inform risk stratification in pediatric acute myeloid leukemia (AML). We describe the first case of a newborn with leukemia cutis found to have AML harboring a cryptic insertional t(8;16)(p11.2;p13.3) with associated KAT6A/CREBBP fusion identified exclusively by fluorescence in situ hybridization (FISH). Expectant management resulted in spontaneous leukemia resolution. The identification of t(8;16)(p11.2;p13.3) may serve as a biomarker for spontaneous remission in congenital AML. FISH for this translocation is warranted in congenital AML with a normal karyotype, and patients with KAT6A/CREBBP fusion should be conservatively managed. While 50% of spontaneously remitting congenital AML with t(8;16)(p11.2;p13.3) may recur, high salvage rates are attained with standard therapy.
Reliability of intraoperative frozen section for the diagnosis of renal tumors suspicious for malignancy in children and adolescents
Authors: Carrasco Alonso , Caldwell Brian T. , Cost Carrye R. , Greffe Brian S. , Garrington Timothy P. , Bruny Jennifer L. , Black Jennifer O. , Cost Nicholas G. 
Published: January 2017

AbstractBackgroundThe ability of intraoperative frozen section (IFS) to reliably diagnose renal tumors in children and adolescents is largely unknown. The objective of our study is to evaluate the ability of IFS to establish a histologic diagnosis for renal tumors in this population.MethodsWe reviewed our experience with patients who underwent IFS at the time of surgery for a renal tumor suspicious for malignancy from 2005 to 2015. The IFS was compared to the final pathology (FP). Data on concordance and reliability were analyzed.ResultsOne hundred thirty patients underwent surgical interventions for a renal tumor suspicious for malignancy, and 32 (25%) patients underwent IFS. Median turnaround time for IFS was 20 min (range 13–44). The histologic IFS diagnosis correlated with FP in 26 (81.2%) cases was discrepant in three (9.4%) cases, and IFS was deferred to FP in three (9.4%) cases (kappa 0.71, 95% confidence interval [CI]: 0.52–0.899, P < 0.001). The IFS correctly distinguished between Wilms tumor and non‐Wilms tumor in 30 (94%) cases (kappa 0.874, 95% CI: 0.705–1, P < 0.001). A total of 17 of 19 (89.5%) Wilms tumors were correctly diagnosed by IFS, yielding a sensitivity of 0.89 (95% CI: 0.67–0.99) and a specificity of 1 (95% CI: 0.75–1).ConclusionIFS is a reliable tool to establish a histologic diagnosis and to differentiate between Wilms and non‐Wilms tumors in children and adolescents with renal tumors. The use of IFS should be encouraged in cases in which obtaining a diagnosis will provide guidance for important “real‐time” medical decision making, specifically additional adjunctive surgical procedures.
Characterization and Management of Patients with Heroin versus Nonheroin Opioid Overdoses: Experience at an Academic Medical Center
Authors: Morizio Kate M. , Baum Regan A. , Dugan Adam , Martin Julia E. , Bailey Abby M. 
Published: January 2017

Study ObjectivesTo characterize the differences between patients who had heroin and nonheroin opioid overdoses and to determine whether there were any significant differences in their management with regard to the naloxone use.DesignRetrospective cohort study.SettingLarge academic medical center.PatientsA total of 923 patients admitted to the medical center who were identified for overdose by heroin or other opiate‐related narcotics between January 2010 and September 2015; 480 patients experienced a nonheroin opioid overdose event, and 443 patients experienced a heroin overdose event.Measurements and Main ResultsPatients presenting with heroin overdose tended to be younger and male, with higher rates of hepatitis C virus (HCV) infection compared with those presenting with nonheroin opioid overdose (p<0.05). Patients in the heroin group were also more likely to have a previous overdose event, history of injection drug use, and history of prescription opioid abuse compared with the nonheroin group (p<0.05). Those presenting with heroin overdose were more likely to receive naloxone in the prehospital setting (p<0.05) but were less likely to receive naloxone once admitted (p<0.05). Patients with nonheroin opioid overdoses required more continuous infusions of naloxone (p<0.05) and admission to the intensive care unit (p<0.05). Of all 923 patients, 178 (19.3%) had a repeat admission for any reason, and 70 (7.6%) were readmitted over the course of the study period for another overdose event with the same drug. The proportion of patients presenting with a heroin overdose steadily increased from 2010–2015; the number of patients presenting to the emergency department with nonheroin opioid overdoses steadily decreased. As rates of heroin overdose increased each year, the incidence of HCV infection increased dramatically.ConclusionThis study indicates that the incidence of heroin overdoses has significantly increased over the last several years, and the rates of HCV infection 4‐fold since the start of the study period. Patients admitted for nonheroin opioid overdose were more likely to be admitted to the hospital and intensive care unit compared with those admitted for heroin overdose. The rise in overdose events only further illustrates a gap in our understanding of the cycle of addiction, drug abuse, and overdose events.
Distinguishing between biochemical and cellular function: Are there peptide signatures for cellular function of proteins?
Authors: Jain Shruti , Bhattacharyya Kausik , Bakshi Rachit , Narang Ankita , Brahmachari Vani 
Published: January 2017

ABSTRACTThe genome annotation and identification of gene function depends on conserved biochemical activity. However, in the cell, proteins with the same biochemical function can participate in different cellular pathways and cannot complement one another. Similarly, two proteins of very different biochemical functions are put in the same class of cellular function; for example, the classification of a gene as an oncogene or a tumour suppressor gene is not related to its biochemical function, but is related to its cellular function. We have taken an approach to identify peptide signatures for cellular function in proteins with known biochemical function. ATPases as a test case, we classified ATPases (2360 proteins) and kinases (517 proteins) from the human genome into different cellular function categories such as transcriptional, replicative, and chromatin remodelling proteins. Using publicly available tool, MEME, we identify peptide signatures shared among the members of a given category but not between cellular functional categories; for example, no motif sharing is seen between chromatin remodelling and transporter ATPases, similarly between receptor Serine/Threonine Kinase and Receptor Tyrosine Kinase. There are motifs shared within each category with significant E value and high occurrence. This concept of signature for cellular function was applied to developmental regulators, the polycomb and trithorax proteins which led to the prediction of the role of INO80, a chromatin remodelling protein, in development. This has been experimentally validated earlier for its role in homeotic gene regulation and its interaction with regulatory complexes like the Polycomb and Trithorax complex. Proteins 2017; 85:682–693. © 2016 Wiley Periodicals, Inc.
Structural role of a conserved active site cis proline in the Thermotoga maritima acetyl esterase from the carbohydrate esterase family 7
Authors: Singh Mrityunjay K. , Manoj Narayanan 
Published: January 2017

ABSTRACTA conserved cis proline residue located in the active site of Thermotoga maritima acetyl esterase (TmAcE) from the carbohydrate esterase family 7 (CE7) has been substituted by alanine. The residue was known to play a crucial role in determining the catalytic properties of the enzyme. To elucidate the structural role of the residue, the crystal structure of the Pro228Ala variant (TmAcEP228A) was determined at 2.1 Å resolution. The replacement does not affect the overall secondary, tertiary, and quaternary structures and moderately decreases the thermal stability. However, the wild type cis conformation of the 227–228 peptide bond adopts a trans conformation in the variant. Other conformational changes in the tertiary structure are restricted to residues 222–226, preceding this peptide bond and are located away from the active site. Overall, the results suggest that the conserved proline residue is responsible for the cis conformation of the peptide and shapes the geometry of the active site. Elimination of the pyrrolidine ring results in the loss of van der Waals and hydrophobic interactions with both the alcohol and acyl moeities of the ester substrate, leading to significant impairment of the activity and perturbation of substrate specificity. Furthermore, a cis‐to‐trans conformational change arising out of residue changes at this position may be associated with the evolution of divergent activity, specificity, and stability properties of members constituting the CE7 family. Proteins 2017; 85:694–708. © 2016 Wiley Periodicals, Inc.
Relaxation of quantum dots in a magnetic field at finite bias – Charge, spin, and heat currents
Authors: Vanherck Joren , Schulenborg Jens , Saptsov Roman B. , Splettstoesser Janine , Wegewijs Maarten R. 
Published: January 2017

AbstractWe perform a detailed study of the effect of finite bias and magnetic field on the tunneling‐induced decay of the state of a quantum dot by applying a recently discovered general duality [Phys. Rev. B 93, 81411 (2016)]. This duality provides deep physical insight into the decay dynamics of electronic open quantum systems with strong Coulomb interaction. It associates the amplitudes of decay eigenmodes of the actual system to the eigenmodes of a so‐called dual system with attractive interaction. Thereby, it predicts many surprising features in the transient transport and its dependence on experimental control parameters: the attractive interaction of the dual model shows up as sharp features in the amplitudes of measurable time‐dependent currents through the actual repulsive system. In particular, for interacting quantum dots, the time‐dependent heat current exhibits a decay mode that dissipates the interaction energy and that is tied to the fermion parity of the system. We show that its decay amplitude has an unexpected gate‐voltage dependence that is robust up to sizable bias voltages and then bifurcates, reflecting that the Coulomb blockade is lifted in the dual system. Furthermore, combining our duality relation with the known Iche‐duality, we derive new symmetry properties of the decay rates as a function of magnetic field and gate voltage. Finally, we quantify charge‐ and spin‐mode mixing due to the magnetic field using a single mixing parameter.
Drugs Against Neurodegenerative Diseases: Design and Synthesis of 6‐Amino–substituted Imidazo[1,2‐b]pyridazines as Acetylcholinesterase Inhibitors
Authors: Sridhar P. , Alagumuthu Manikandan , Ram B. , Arumugam Sivakumar , Reddy Sabbasani Rajasekhara 
Published: January 2017

Abstract3‐nitro‐6‐amino substituted –imidazo [1,2‐b]pyridazine derivatives (5a–5 l) were synthesized in four steps and characterized by FT‐IR, 1H NMR, 13C NMR and HRMS. 3‐nitro‐6‐amino substituted –imidazo [1,2‐b]pyridazine derivatives (5a‐l) was evaluated for the acetylcholinesterase (AChE) inhibition and antioxidant activities. In both the studies, 3‐nitro‐6‐amino substituted –imidazo [1,2‐b]pyridazine derivatives (5j‐l) were inactive. 3‐nitro‐6‐(piperidin‐1‐yl)imidazo[1,2‐b]pyridazine (5c), substituted with piperidine and 3‐nitro‐6‐(4‐phenylpiperazin‐1‐yl) imidazo[1,2‐b] pyridazine (5 h), substituted with 1‐phenylpiperazine were the most potent compounds (IC50 <0.05 μM for AChE inhibition activity). Latterly, the most potent compounds 3‐nitro‐6‐(4‐phenylpiperazin‐1‐yl) imidazo[1,2‐b] pyridazine (5 h), 3‐nitro‐6‐(piperidin‐1‐yl)imidazo[1,2‐b]pyridazine (5c), and moderately active compounds 3‐nitro‐6‐(pyrrolidin‐1‐yl)imidazo[1,2‐b]pyridazine (5b), 6‐morpholino‐3‐nitroimidazo[1,2‐b]pyridazine (5d), 1‐(3‐nitroimidazo[1,2‐b]pyridazin‐6‐yl)piperidine‐4‐carbonitrile (5e), 6‐(4‐ethylpiperazin‐1‐yl)‐3‐nitroimidazo [1,2‐b]pyridazine (5 g), Tert‐butyl 4‐(3‐nitroimidazo[1,2‐b] pyridazin‐6‐yl) piperazine‐1‐carboxylate (5i) were selected for in vivo study.