bluuebunny/crossref_metadata_2025_split huggingface dataset:
48-spot single-molecule FRET setup with periodic acceptor excitation
Authors: Ingargiola Antonino , Segal Maya , Gulinatti Angelo , Rech Ivan , Labanca Ivan , Maccagnani Piera , Ghioni Massimo , Weiss Shimon , Michalet Xavier 
Published: June 2017

Single-molecule FRET (smFRET) allows measuring distances between donor and acceptor fluorophores on the 3-10 nm range. Solution-based smFRET allows measurement of binding-unbinding events or conformational changes of dye-labeled biomolecules without ensemble averaging and free from surface perturbations. When employing dual (or multi) laser exci-tation, smFRET allows resolving the number of fluorescent labels on each molecule, greatly enhancing the ability to study heterogeneous samples. A major drawback to solution-based smFRET is the low throughput, which renders repetitive measurements expensive and hin-ders the ability to study kinetic phenomena in real-time.Here we demonstrate a high-throughput smFRET system which multiplexes acquisition by using 48 excitation spots and two 48-pixel SPAD array detectors. The system employs two excitation lasers allowing separation of species with one or two active fluorophores. The performance of the system is demonstrated on a set of doubly-labeled double-stranded DNA oligonucleotides with different distances between donor and acceptor dyes along the DNA duplex. We show that the acquisition time for accurate subpopulation identification is reduced from several minutes to seconds, opening the way to high-throughput screening applications and real-time kinetics studies of enzymatic reactions such as DNA transcription by bacterial RNA polymerase.
Widespread translational remodeling during human neuronal differentiation
Authors: Blair John D. , Hockemeyer Dirk , Doudna Jennifer A. , Bateup Helen S. , Floor Stephen N. 
Published: June 2017

AbstractFaithful cellular differentiation requires precise coordination of changes in gene expression. However, the relative contributions of transcriptional and translational regulation during human cellular differentiation are unclear. Here, we induced forebrain neuronal differentiation of human embryonic stem cells (hESCs) and characterized genomewide RNA and translation levels during neurogenesis. We find that thousands of genes change at the translation level across differentiation without a corresponding change in RNA level. Specifically, we identify mTOR complex 1 signaling as a key driver for elevated translation of translation-related genes in hESCs. In contrast, translational repression in active neurons is mediated by transcript 3′ UTRs, through regulatory sequences. Together, our findings identify a functional role for the dramatic 3′ UTR extensions that occur during brain development, and provide insights to interpret genetic variants in post-transcriptional control factors that influence neurodevelopmental disorders and diseases.
Characterization of SID-1-dependent and independent intergenerational RNA transport pathways inCaenorhabditis elegans
Authors: Wang Eddie , Hunter Craig P. 
Published: June 2017

AbstractSystemic RNA interference (RNAi) inC. elegansis dependent onsid-1(Winstonet al.2002),sid-3(Joseet al.2012) andsid-5(Hinaset al.2012). After injection, expression, or ingestion, double-stranded RNA (dsRNA) is transported between cells throughout the animal to enable RNAi in most tissues, including the germline and progeny. Here, we characterize the role of the Sid genes in transport of dsRNA to progeny. We previously reported that dsRNA injected directly in the germline unexpectedly requiressid-1activity in the progeny to initiate RNAi (Winstonet al.2002). We now show that germline injected dsRNA can travel by three independent pathways to silence gene expression in embryos. First, germline injected dsRNA is delivered, presumably by bulk flow, into oocytes and embryos. This means of delivery, which does not requiresid-1,is limited by the amount and location of injected dsRNA. Second, maternalsid-1transports extracellular dsRNA into the germline where it can silence maternal deposited mRNAs and segregate to embryos to silence embryonically expressed mRNAs. Third, extracellular dsRNA is also endocytosed into oocytes by the low-density lipoprotein (LDL) receptor superfamily homolog RME-2. The endocytosed dsRNA then requiressid-1andsid-5in embryos to silence embryonically expressed genes. Extracellular fluorescent dsRNA, once endocytosed into oocytes, does not co-localize with VIT2::GFP and it does not requiresid-1activity to segregate from the late endocytosis marker GFP::RAB-7 in early embryos. In conclusion, we identify genes and pathways that function redundantly for intergenerational RNA transfer that may represent mechanisms for transgenerational epigenetic inheritance.
Cell-type selective deletion of RSK2 reveals insights into altered signaling in Coffin-Lowry Syndrome
Authors: Zhu Hu , Strachan Ryan T. , Urban Daniel J. , Farrell Martilias S. , Kroeze Wesley K. , English Justin G. , Olsen Reid H.J. , Roth Bryan L. 
Published: June 2017

ABSTRACTCoffin-Lowry syndrome (CLS) is an X-linked syndromic form of mental retardation characterized by various skeletal dysmorphisms, moderate to severe mental retardation, and in some cases, psychosis. CLS is caused by loss-of-function mutations of the p90 ribosomal S6 kinase 2 (RPS6KA3) gene encoding a growth factor-regulated serine/threonine kinase, ribosomal S6 kinase 2 (RSK2). We previously identified RSK2 as a novel interacting protein that tonically inhibits 5-HT2Areceptor signaling by phosphorylating Ser-314 within the third intracellular loop. To determine if RSK2 inhibits 5-HT2Areceptor signalingin vivoand whether disruption of RSK2 could lead to schizophrenia-like behaviors - as is seen in some CLS patients - we genetically disrupted the function of RSK2 either globally or selectively in forebrain pyramidal neurons in mice. Both global and forebrain-selective RSK2 deletion augmented the locomotor responses to the psychotomimetic drugs phencyclidine (PCP) and amphetamine (AMPH). Significantly, forebrain-selective deletion of RSK2 augmented 5-HT2Areceptor signaling as exemplified by enhanced 5-HT2A-mediated c-fos activation and head-twitch response without altering the levels or distribution of 5-HT2Areceptor protein. Thus, RSK2 modulates 5HT2Areceptor functionin vivo,and disruption of RSK2 leads to augmented psychostimulant-induced responses reminiscent of those seen in many animal models of schizophrenia. These findings strengthen the association between 5-HT2Areceptor dysfunction and psychosis, and provide a potential mechanism underlying the schizophrenia-like symptoms present in some CLS patients.HighlightsGlobal and cell-type-specific RSK2 knock-out mice were assessed behaviorally and pharmacologicallyAugmentation of amphetamine and PCP locomotor responses were seen in both global and forebrain-specific RSK2 KO miceAugmentation of 5-HT2A serotonin receptor function but not number was also observedThese alterations reveal insights into mechanisms potentially responsible for behavioral sequlae of Coffin-Lowry Syndrome
Profit
Authors: Cowton Chris 
Published: June 2017

Purpose
The purpose of this poem is to allow the reader to contemplate the substitution and to decide whether it works.

Design/methodology/approach
A short “poem” based on the idea of replacing “love” with “profit” in St Paul’s famous passage in 1 Corinthians 13, and often read at weddings. The word “charity” would appear instead of “love” if the old King James Version of the Bible were used instead.

Findings
An inference might be drawn that love is better than profit.

Originality/value
The poem encourages a comparison between profit and love.
Global perspectives on top management team pay structures
Authors: Brown Mark , Minsky Barbara , Voss Richard , Ozgen Eren 
Published: June 2017

PurposeThe purpose of this paper is to investigate the relation between countries’ values of individualism/collectivism and organizations’ top management team (TMT) pay structures. Individualistic countries are expected to prefer more hierarchical TMT pay structures and collectivist countries are expected to prefer more egalitarian TMT pay structures. The manuscript also investigates the international implications of the relation between TMT pay structures and organizational performance. Specifically, it is proposed that a country’s level of individualism/collectivism will mediate the relation between TMT pay structure hierarchy and organizational performance.Design/methodology/approachA pooled sample of data from 56 organizations in 12 countries was used to investigate the research questions. Individualism/collectivism was measured using country specific individualism/collectivism scores and top management pay structures were operationalized using Gini coefficients. Organizational performance was evaluated using return on assets.FindingsSupport was found both for a preference for more hierarchical TMT pay structures in individualistic countries, and that a country’s level of individualism/collectivism mediates the relationship between an organization’s top management’s pay structure and company performance.Originality/valueFindings demonstrate that organizations use pay structures consistent with their environments. Results suggest cultural dimensions can contribute to understanding cross-national TMT pay structures and that national culture plays a significant role in the relationship between TMT pay structure and company performance.
Russian agon and the quest for theoretical sociology
Authors: Kulikov Sergey B. 
Published: June 2017

Purpose
The purpose of this paper is to represent an epistemological analysis of Russian sociological scholarship.

Design/methodology/approach
The analytical approach that allows reducing the particular representations within the sum of propositions is the methodological base of the paper. Clearing of propositional attitudes explains the basic communications in a thought of researchers. The circle of grounds available to achievement of intuitively noticed purposes defines the preferences of researchers in general.

Findings
The author proves that the theoretical developments in the Russian sociology are possible as a derivative from the development of questions, which are raised nowadays in worldwide science, but possible in a view of original development of questions, which were raised in worldwide science in the past.

Research limitations/implications
The Russian sociology represents a part of the European humanities, which is based on the various forms of theoretical combat or agonality.

Practical implications
The author shows the ways out of the theoretical combat or agonality.

Social implications
The research clarifies the perspectives for increasing of the knowledge-based society in Russia. The author analyzes the concept of Russia as a paradigmatic society, particularly in the context of transition economies.

Originality/value
In general, the author concludes that the pro-argument with respect to theoretical developments is weaker than the contra-argument with respect to theoretical developments. This paper has revealed the model within which extreme positions can be reconciled.
Grey relational analysis coupled with principal component analysis for optimization of the cyclic parameters of a solar-driven organic Rankine cycle
Authors: Tiwari Deepak , Sherwani Ahmad Faizan , Asjad Mohammad , Arora Akhilesh 
Published: June 2017

Purpose
The purpose of this paper is to investigate the effect of four controllable parameters (fuel mixture, evaporation bubble point temperature, expander inlet temperature and condensation dew point temperature) of a solar-driven organic Rankine cycle (ORC) on the first-law efficiency, the exergetic efficiency, the exergy destruction and the volume flow ratio (expander outlet/expander inlet).

Design/methodology/approach
Nine experiments as per Taguchi’s standard L9 orthogonal array were performed on the solar-driven ORC. Subsequently, multi-response optimization was performed using grey relational and principal component analyses.

Findings
The results revealed that the grey relational analysis along with the principal component analysis is a simple as well as effective method for solving the multi-response optimization problem and it provides the optimal combination of the solar-driven ORC parameters. Further, the analysis of variance was also employed to identify the most significant parameter based on the percentage of contribution of each cyclic parameter. Confirmation tests were performed to check the validity of the results which revealed good agreement between predicted and experimental values of the response variables at optimum combination of the input parameters. The optimal combination of process parameters is the set with A3 (the best fuel mixture in the context of optimal performance is 0.9 percent butane and 0.1 percent pentane by weight), B2 (evaporation bubble point temperature=358 K), C1 (condensation dew point temperature=300 K) and D3 (expander inlet temperature=370 K).

Research limitations/implications
In this research, the Taguchi-based grey relational analysis coupled with the principal components analysis has been successfully carried out, whereas for any optimized solution, it is required to have a real-time scenario that may be taken into consideration by the application of different soft computing techniques like genetic algorithm, simulated annealing, etc. The results generated are purely based on theoretical modeling, and, for further research, experimental analyses are required to consolidate the generated results.

Originality/value
This piece of research work will be helpful to users of solar energy, academicians, researchers and other concerned persons, in understanding the importance, severity and benefits obtained by the application, implementation and optimization of the cyclic parameters of the solar-driven ORC.
Grey-based model for forecasting Mauritius international tourism from different regions
Authors: Pirthee Medha 
Published: June 2017

Purpose
The purpose of this paper is to understand the trend and forecast the number of tourists from different regions of the world to Mauritius.

Design/methodology/approach
The paper adopts two grey system models, the even model GM(1,1) and the non-homogeneous discrete grey model (NDGM), to forecast the total number of international tourism to Mauritius and its structure from different regions tourist arrivals to Mauritius for the next three years. Grey system theory models were used to account for uncertainties and the dynamism of the tourism sector environment. The two models were applied as a comparison to obtain more reliable forecasting figures.

Findings
The results demonstrate that both of the grey system models can be successfully applied with high accuracy for Mauritian tourism prediction, and also the number of tourist arrivals to Mauritius shows a continued augmentation for the upcoming years.

Practical implications
Forecasting is meaningful since the Government of Mauritius, private companies or any concerned authority can adopt the forecasting methods exposed in this paper for the development of the tourism sector through managerial and economic decision making.

Originality/value
Mauritius is a charming travel destination. Through this paper, it can be seen that future tourism travel to Mauritius has been successfully predicted based on previous data. Moreover, it seems that the grey system theory models have not been utilised yet as forecasting tools for the tourism sector of Mauritius as opposed to other countries such as China and Taiwan.
Explanations about grey information and framework of grey system modeling
Authors: Xie Naiming 
Published: June 2017

Purpose
The purpose of this paper is to summarize the different types of grey information, explain the mechanism of grey system modeling and reconstruct the framework of grey system theory (GST).

Design/methodology/approach
GST has been developed for more than three decades; however, the framework of GST is still in an evolutionary process. This manuscript first explains grey information in detail, and then summarizes a series of grey system models under limited data and poor information. Figures and general steps for different types of grey system models are provided in this paper.

Findings
The findings in this paper clearly differentiate between grey information and other uncertainty information. The differences between grey system models and other uncertainty models are clearly explained. In addition, general steps for different grey system models are given which demonstrate the orientation of grey system modeling.

Practical implications
Theoretical framework is very important for developing a new theory. This paper clarified grey information and grey system-based modeling mechanism. It is very useful to understand and explain the systematic framework of GST and it contributes undoubtedly to make GST perfect.

Originality/value
Grey information is explained in terms of limited data and two types of grey numbers. Accordingly, all of the grey system models were divided into limited data-based grey system models and grey number-based grey system models.
Algorithm rules of interval grey numbers based on different “kernel” and the degree of greyness of grey numbers
Authors: Guo San-dang , Liu Sifeng , Fang Zhigeng 
Published: June 2017

Purpose
The purpose of this paper is to establish the algorithm rules of the interval grey numbers and propose a new ranking method of the interval grey numbers.

Design/methodology/approach
The definitions of “kernels” based on lower measure, upper measure or moderate measure are given according to the properties of the interval grey number problems. By means of the measurement error, the concept of the absolute degree of greyness and the relative degree of greyness corresponding to different “kernel” are given, and different simplified forms of the interval grey numbers are put forward.

Findings
The definitions of “kernel” and the degree of greyness in this paper not only take the upper limit, lower limit and the coverage of the interval grey numbers into account, but also avoid the inconsistency of the degree of greyness caused by the different universe of discourse.

Research limitations/implications
Though the method proposed in this paper has some deficiencies, such as the definition of relative degree of greyness is meaningless when the kernel of the interval grey number is 0, it provides a new idea for calculating and sorting the interval grey numbers and is conducive to the further development of the grey system theory.

Originality/value
The method proposed in this paper can not only distinguish interval grey numbers in different situations, but also avoid the inconsistency of the degree of greyness caused by the different universe of discourse. In this basis, the interval grey number algorithm is established and a new ranking method of interval grey numbers is given.
Value creation through reverse logistics in used clothing networks
Authors: Pal Rudrajeet 
Published: June 2017

Purpose
The purpose of this paper is to identify the major reverse logistics design aspects in used clothing value chains, and those enabling and challenging manifestation of value creation.

Design/methodology/approach
This research is based on an exploratory study of 12 established organizations in Swedish used clothing networks. Data were gathered through semi-structured interviews, secondary sources, and subsequent field visits.

Findings
Empirical insights on how various design aspects influence value creation in used clothing value chains are provided. Crucial among these are strategic and consistent collection, presence of multi-channel sales, and communication of post-retail concepts for manifesting value by bolstering consumer satisfaction, environmental motivation, and corporate image. Inter-organizational collaboration in reverse logistics processes and workplace training, further renders higher economic, environmental and information values.

Research limitations/implications
The paper proposes a holistic framework of design aspects in reverse value chains, and extends existing knowledge on how these aspects manifest value creation. By doing so, a nuanced view of the design aspects is offered by highlighting how they can differentially, either enable, or challenge value creation. In this connection, seven supporting propositions are developed for in-depth future research.

Practical implications
The paper includes implications for the devising strategic solutions for higher value creation, by understanding of the key enablers and challenges, for many actors in the used clothing networks.

Originality/value
The role of various design aspects in reverse value chains for manifesting multifaceted stakeholder value creation is explicitly defined in the paper.
Stress in paid and unpaid work as related to cortisol and subjective health complaints in women working in the public health care sector
Authors: Blom Victoria , Svedberg Pia , Bergström Gunnar , Mather Lisa , Lindfors Petra 
Published: June 2017

Purpose
Focusing on 420 women employed within the woman-dominated health care sector, the purpose of this paper is to investigate how any variation in their total workload (TWL) in terms of paid and unpaid work relate to various subjective health complaints (SHC) (n=420) and the neuroendocrine stress marker cortisol (n=68).

Design/methodology/approach
The authors explored how any variation in their TWL in terms of paid and unpaid work related cross-sectionally to SHC (n=420), and the neuroendocrine stress marker cortisol (n=68).

Findings
Hierarchical regression analyses showed that stress of unpaid work was most strongly related to diurnal variations in cortisol. Both stress of paid and unpaid work as well as TWL stress, but not hours spent on TWL, were related to SHC.

Practical implications
Taken together, objective measures of hours spent on various TWL domains were unrelated to outcome measures while perceptions of having too much TWL and TWL stress were linked to both cortisol and SHC, i.e. how individuals perceive a situation seem to be more important for health than the actual situation, which has implications for research and efforts to reduce individual TWL.

Originality/value
This study is unique in showing that unpaid work and perceptions having too much TWL relate to stress markers in women working in the public health care sector.