bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure to chlorantraniliprole affects the energy metabolism of the caddisfly Sericostoma vittatum
Authors: Rodrigues Andreia C.M. , Gravato Carlos , Quintaneiro Carla , Bordalo Maria D. , Golovko Oksana , Žlábek Vladimír , Barata Carlos , Soares Amadeu M.V.M. , Pestana João L.T. 
Published: November 2016

Abstract

Caddisflies have been included in ecotoxicological studies because of their sensitivity and ecological relevance. The present study aimed to assess the sublethal effects of an anthranilic diamide insecticide, chlorantraniliprole (CAP), to Sericostoma vittatum. Used worldwide, CAP is a persistent compound that has been found in surface waters at concentrations from 0.1 μg/L to 9.7 μg/L. It targets the ryanodine receptors, and the present ecotoxicological assessment focused on biomarkers related to neurotransmission, biotransformation, oxidative stress damage, and endpoints related to energy processing (feeding, energy reserves, and cellular metabolism). Six days of exposure trials revealed that feeding activity was significantly decreased in S. vittatum larvae exposed to 0.9 μg/L CAP. Concomitantly, a reduction in cellular metabolism and a significant decrease in protein content were also observed in caddisfly larvae exposed to CAP, suggesting metabolic depression. The results show that sublethal concentrations of CAP can cause detrimental sublethal effects on S. vittatum total glutathione content at concentrations as low as 0.2 μg/L. Bioenergetics can be used to assess physiological effects of contaminants, and the present results show that exposure to low, environmentally relevant, concentrations of CAP alter energy acquisition and metabolism in nontarget aquatic insects with potential population level effects. Environ Toxicol Chem 2017;36:1584–1591. © 2016 SETAC
Waterproof Breathable Membrane Used as Gas Diffusion Layer in Activated Carbon Air Cathode Microbial Fuel Cells
Authors: Fu Z. , Li K. , Pu L. , Ge B. , Chen Z. 
Published: November 2016

AbstractOne of the main limiting factors for scaling up microbial fuel cells (MFCs) technology is to develop low‐cost and high‐efficiency cathode. A new and simplified approach was developed by using a commercial waterproof breathable membrane (WBM) as gas diffusion layer (GDL) material as substitution for conventional polytetrafluoroethylene (PTFE) GDL. Air‐cathode with the WBM pasted (AC‐P) onto the stainless steel mesh (SSM) achieved a maximum power density of 611 ± 10 mWm−2, which was similar to that using a PTFE GDL by rolling method (645 ± 12 mWm−2, AC‐R). Physical and electrochemical techniques were employed to investigate the morphology and electrochemical characteristics of the cathode. The result demonstrated that AC‐P had a higher current density and internal resistance than AC‐R. Besides, the WBM had a higher porosity and uniform texture. The study showed that the WBM was a kind of good GDL material for easy preparation, low cost and stable performance of cathode construction.
PreCimp: Pre‐collapsing imputation approach increases imputation accuracy of rare variants in terms of collapsed variables
Authors: Kim Young Jin , Lee Juyoung , Kim Bong‐Jo ,   T2D‐Genes Consortium, Park Taesung 
Published: November 2016

ABSTRACTImputation is widely used for obtaining information about rare variants. However, one issue concerning imputation is the low accuracy of imputed rare variants as the inaccurate imputed rare variants may distort the results of region‐based association tests. Therefore, we developed a pre‐collapsing imputation method (PreCimp) to improve the accuracy of imputation by using collapsed variables. Briefly, collapsed variables are generated using rare variants in the reference panel, and a new reference panel is constructed by inserting pre‐collapsed variables into the original reference panel. Following imputation analysis provides the imputed genotypes of the collapsed variables. We demonstrated the performance of PreCimp on 5,349 genotyped samples using a Korean population specific reference panel including 848 samples of exome sequencing, Affymetrix 5.0, and exome chip. PreCimp outperformed a traditional post‐collapsing method that collapses imputed variants after single rare variant imputation analysis. Compared with the results of post‐collapsing method, PreCimp approach was shown to relatively increase imputation accuracy about 3.4–6.3% when dosage r2 is between 0.6 and 0.8, 10.9–16.1% when dosage r2 is between 0.4 and 0.6, and 21.4 ∼ 129.4% when dosage r2 is below 0.4.
Fundamentals of the vibratory driving of piles and sheet piles
Authors: Massarsch K. Rainer , Fellenius Bengt H. , Bodare Anders 
Published: November 2016

AbstractVibratory driving is a common method for installing or extracting piles and sheet piles as well as for deep vibratory compaction. The most important parameters are vibration frequency, vibration amplitude and eccentric moment. These parameters govern vibratory driving and, in particular, the soil resistance at the toe and along the shaft of a pile. Pile driving causes oscillating horizontal ground vibrations in coarse‐grained soils. It can be shown that these horizontal vibrations reduce the shaft resistance during driving. The process results in a permanent increase in the horizontal effective stress, which causes arching around the vibrated pile. The increase in horizontal effective stress is also important for deep vibratory compaction. The resonance frequency of the vibrator‐pile‐soil system significantly affects pile penetration and the emission of ground vibrations. At resonance, the vertical vibration velocity in the soil reaches a maximum and pile penetration becomes very slow, whereas beyond resonance, the vibration velocity decreases and the pile penetration speed is high. Field monitoring of the vibratory driving process can be used to optimize vibratory pile driving, as examples have shown. A new concept is proposed in which the driveability can be determined from a correlation between penetration resistance measurements (blows/depth) and penetration speed. The validity of the concept is demonstrated by a case history and project references.
The Effect on Mental Health of Retiring During the Economic Crisis
Authors: Belloni Michele , Meschi Elena , Pasini Giacomo 
Published: November 2016

AbstractThis paper investigates the causal impact of retirement on late‐life mental health, a growing concern for public health, because major depressive disorders are the second leading cause of disability. We shed light on the role of economic conditions in shaping the effect of retirement on mental health by exploiting time and regional variation in the severity of the economic crisis across 10 European countries during 2004–2013. We use data from four waves of the Survey of Health, Ageing and Retirement in Europe and address the potential endogeneity of the retirement decision to mental health by applying a fixed‐effects instrumental variables approach. The results indicate that retirement improves the mental health of men but not that of women. This effect is stronger for blue‐collar men working in regions that have been severely hit by the economic crisis. These findings may be explained by the worsening of working conditions and the rise in job insecurity stemming from the economic downturn: under these circumstances, exit from the labour force is perceived as a relief. Copyright © 2016 John Wiley & Sons, Ltd.
Combinatorial optimization of resilience engineering and organizational factors in a gas refinery by a unique mathematical programming approach
Authors: Azadeh A. , Salehi V. , Mirzayi M. , Roudi E. 
Published: November 2016

AbstractThis study is an attempt to evaluate the mutual impacts of resilience engineering (RE) and managerial and organizational factors in a large gas refinery. RE is a paradigm for safety management that focuses on how to help people cope with complexity under pressure to achieve success. The data obtained from questionnaire are analyzed by statistical methods and data envelopment analysis (DEA) approach. The results of Cronbach's alpha test show that the data are reliable. The results of DEA demonstrate that learning and flexibility among all RE factors have the greatest impact on managerial and organizational factors. In addition, the organizational factors have a greater influence on RE in comparison with managerial factors. Spearman's test was used to verify the results of this study. This is the first study that identifies the most important factors of integrated managerial and organizational factors and RE through a unique mathematical programming approach in a gas refinery.
Membrane Binding of Parkinson's Protein α‐Synuclein: Effect of Phosphorylation at Positions 87 and 129 by the S to D Mutation Approach
Authors: Kumar Pravin , Schilderink Nathalie , Subramaniam Vinod , Huber Martina 
Published: November 2016

AbstractHuman α‐synuclein, a protein relevant in the brain with so‐far unknown function, plays an important role in Parkinson's disease. The phosphorylation state of αS was related to the disease, prompting interest in this process. The presumed physiological function and the disease action of αS involves membrane interaction. Here, we study the effect of phosphorylation at positions 87 and 129, mimicked by the mutations S87A, S129A (nonphosphorylated) and S87D, S129D (phosphorylated) on membrane binding. Local binding is detected by spin‐label continuous‐wave electron paramagnetic resonance. For S87A/D, six positions (27, 56, 63, 69, 76, and 90) are probed; and for S129A/D, three (27, 56, and 69). Binding to large unilamellar vesicles of 100 nm diameter of 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) and 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine in a 1 : 1 composition is not affected by the phosphorylation state of S129. For phosphorylation at S87, local unbinding of αS from the membrane is observed. We speculate that modulating the local membrane affinity by phosphorylation could tune the way αS interacts with different membranes; for example, tuning its membrane fusion activity.
An Appreciation of Organic Solid‐State Chemistry and Challenges in the Field of “Molecules, Materials, Medicines”
Authors: Jones William 
Published: November 2016

AbstractOver many years, much of what has motivated a considerable amount of research in the general area of organic solid‐state chemistry concerns an understanding of how molecular packing within an organic solid affects its physical and chemical properties. Whilst the motivation for this understanding may have changed over time, the fundamental issues remain the same. An understanding of the effect of crystal attributes (e.g., polymorph, habit and particle size) on chemical, physical, photophysical and electronic properties is still vital to the development of organic solids with optimised properties. While progress has been made in analytical skills (including sophisticated developments in instrumentation), along with major developments in computational techniques, there are still many challenges. This paper, with a primary focus on pharmaceuticals, provides a brief, and not comprehensive, personal overview of the progress that has been made and has yet to be made. Amongst the chemists and crystallographers who have contributed significantly to the subject is Jack Dunitz and some of his seminal papers appear in several of the issues discussed.
The Development of Alkoxide‐Directed Metallacycle‐Mediated Annulative Cross‐Coupling Chemistry
Authors: Micalizio Glenn C. , Mizoguchi Haruki 
Published: November 2016

AbstractAlkoxide‐directed metallacycle‐mediated cross‐coupling is a rapidly growing area of reaction methodology in organic chemistry. Over the last decade, developments have resulted in more than thirty new and highly selective intermolecular (or “convergent”) C−C bond‐forming reactions that have established powerful retrosynthetic relationships in stereoselective synthesis. While early studies were focused on developing transformations that forge a single C−C bond by way of a functionalized and unsaturated metallacyclopentane intermediate, recent advances mark the ability to employ this organometallic intermediate in additional stereoselective transformations. Among these more advanced coupling processes, those that embrace the metallacycle in subsequent [4+2] chemistry have resulted in the realization of a number of highly selective annulative cross‐coupling reactions that deliver densely functionalized and angularly substituted carbocycles. This review discusses the early development of this chemistry, recent advances in reaction methodology, and shares a glimpse of the power of these processes in natural product synthesis.
Synthesis of the Right‐Side Structure of Type B Physalins
Authors: Morita Masaki , Kojima Shuntaro , Ohkubo Megumi , Koshino Hiroyuki , Hashizume Daisuke , Hirai Go , Maruoka Keiji , Sodeoka Mikiko 
Published: November 2016

AbstractWe present a full account of our synthetic studies on the racemic DEFGH‐ring moiety of physalins, featuring domino ring transformation of a tricyclic key intermediate. We also report the results of a detailed mechanistic examination of the domino ring transformation, as well as a reoptimization of the 2,3‐Wittig rearrangement and methylation steps. Furthermore, we have newly established a method for the preparation of an optically active synthetic intermediate by enzymatic kinetic resolution. Our work provides access to both natural and nonnatural right‐side physalin structures.
Nrf2 Activation as a Protective Feedback to Limit Cell Death in High Glucose‐Exposed Cardiomyocytes
Authors: Tsai Cheng‐Yen , Wen Su‐Ying , Cheng Shi‐Yann , Wang Chung‐Hsing , Yang Yao‐Chih , Viswanadha Vijaya Padma , Huang Chih‐Yang , Kuo Wei‐Wen 
Published: November 2016

ABSTRACTHyperglycemia leads to excess reactive oxygen species (ROS) generation, which causes many diabetic complications, such as cardiomyopathy. Nuclear factor erythroid 2‐related factor 2 (Nrf2), a redox‐sensing transcription factor, can up‐regulate its downstream antioxidant gene expressions in response to oxidative stress. However, the regulatory signal pathway in which high glucose (HG) induces Nrf2 activation is still unclear. Our results demonstrated that HG (33 mM) can indeed stimulate Nrf2 protein expression and translocation into the nucleus in cardiomyocytes, enhancing the downstream antioxidant protein levels. Using siRNAs, p38, JNK, PKCα, and PKCδ, as well as ROS scavengers, it was observed that the dependence of PKCα/PKCδ on ROS production to enhance JNK and p38 phosphorylation mediated HG‐induced cardiac Nrf2 expression and activation. Knockdown of Nrf2 by siRNA transfection increased cleaved‐caspase3, reduced Bcl2 in the cellular protein level and further exacerbated HG‐induced apoptosis. In addition, all of these proteins induced by HG in vitro were also increased in STZ‐induced diabetic rat ventricles in vivo. Our study demonstrated that HG‐induced cardiac Nrf2 activation occurs through PKCα/PKCδ—ROS—JNK/p38 signaling. These findings may provide a therapeutic target to counteract the oxidative stress associated with diabetic cardiomyopathy. J. Cell. Biochem. 118: 1659–1669, 2017. © 2016 Wiley Periodicals, Inc.
Placental‐Specific Overexpression of sFlt‐1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model
Authors: Kühnel Elisabeth , Kleff Veronika , Stojanovska Violeta , Kaiser Stephanie , Waldschütz Ralph , Herse Florian , Plösch Torsten , Winterhager Elke , Gellhaus Alexandra 
Published: November 2016

ABSTRACTSince it is known that placental overexpression of the human anti‐angiogenic molecule sFlt‐1, the main candidate in the progression of preeclampsia, lead to intrauterine growth restriction (IUGR) in mice by lentiviral transduction of mouse blastocysts, we hypothesize that sFlt‐1 influence placental morphology and physiology resulting in fetal IUGR. We therefore examined the effect of sFlt‐1 on placental morphology and physiology at embryonic day 18.5 with histologic and morphometric analyses, transcript analyses, immunoblotting, and methylation studies. Interestingly, placental overexpression of sFlt‐1 leads to IUGR in the fetus and results in lower placental weights. Moreover, we observed altered trophoblast differentiation with reduced expression of IGF2, resulting in a smaller placenta, a smaller labyrinth, and the loss of glycogen cells in the junctional zone. Changes in IGF2 are accompanied by small changes in its DNA methylation, whereas overall DNA methylation is unaffected. In addition, the expression of placental nutrient transporters, such as the glucose diffusion channel Cx26, is decreased. In contrast, the expression of the fatty acid transporter CD36 and the cholesterol transporter ABCA1 is significantly increased. In conclusion, placental sFlt‐1 overexpression resulted in a reduction in the differentiation of the spongiotrophoblast into glycogen cells. These findings of a reduced exchange area of the labyrinth and glycogen stores, as well as decreased expression of glucose transporter, could contribute to the intrauterine growth restriction phenotype. All of these factors change the intrauterine availability of nutrients. Thus, we speculate that the alterations triggered by increased anti‐angiogenesis strongly affect fetal outcome and programming. J. Cell. Biochem. 118: 1316–1329, 2017. © 2016 Wiley Periodicals, Inc.
Amino acid profile and sensory characteristics of dry fermented pork loins produced with a mixture of probiotic starter cultures
Authors: Neffe‐Skocińska Katarzyna , Okoń Anna , Kołożyn‐Krajewska Danuta , Dolatowski Zbigniew 
Published: November 2016

AbstractBACKGROUNDProteolysis is a biochemical process in dry‐aged meat products where proteins are metabolized and broken down to polypeptides, peptides, and free amino acids. In the literature it is reported that an appropriate choice of probiotic starter culture limits proteolytic changes in dry‐fermented meat products. In this study the combined effect of a mixture of probiotic starter cultures on the free amino acid profile, total count of lactic acid bacteria, and the sensory quality of dry‐aged pork loins after fermentation and after storing the vacuum‐packed samples was evaluated.RESULTSLOCK900 and BB12 probiotic strains were the technologically best two‐species mixture of starter cultures for the production of probiotic dry‐aged pork loins. They allowed us to obtain products with high and stable bacterial count and acceptable sensory quality, both after 21 days of fermentation and after 2 months of cold storage. Changes in the free amino acid profile and increased intensity of the selected sensory attributes result from a significant share of probiotics in meat proteolysis occurring during fermentation and storage.CONCLUSIONThe results suggest the relevance of using probiotic bacteria as a two‐species starter culture for the production of dry‐aged products. © 2016 Society of Chemical Industry
Kinetics of the Anaerobic Reaction of para‐Substituted Phenols with Nitrogen Dioxide
Authors: Reyes Jael , Lissi Eduardo , López‐Alarcón Camilo , Rubio María A. 
Published: November 2016

ABSTRACTpara‐Substituted phenols in aqueous solution under anaerobic conditions readily react with nitrogen dioxide (NO2●) over a wide range of experimental conditions. The rate and rate law of the process were dependent on phenol concentration and solution pH. The kinetic order in phenol changed from one (low concentration) to zero (high concentration), a result attributable to total NO2● capture. Initial consumption rate (r0) of phenols versus pH plots showed parabolic behavior with a minimum rate at pH ca. 5. On the other hand, the maximum rate took place at high pH (pH>10) and involved the protonated phenols. The reaction rate of para‐substituted phenols with NO2● correlated with the bond dissociation energy and with Hammett's parameter. Based on such results and also supported by analysis of products carried out by HPLC‐MS/MS, our data conclusively show that, in spite of the fast acid–base interchanges of phenols and the interconversion of the different nitrogen oxides, the mechanisms of phenols nitration mediated by NO2● or HONO are clearly different.
Photopolymerization Behavior of Coordinated Ionic Liquids Formed from Organic Monomers with Alkali and Alkaline Earth Metal Bistriflimide Salts
Authors: Whitley John W. , Benefield Shellby C. , Liu Haining , Burnette Michael T. , Turner C. Heath , Bara Jason E. 
Published: November 2016

Coordinated ionic liquid (IL) monomers provide many of the benefits of conventional ILs in radical polymerization with the advantage of relatively simple synthesis and recovery of the polymer product. Previous studies have reported high solubilities of lithium bistriflimide in polar organic monomers such as methyl methacrylate (MMA), 1‐vinylimidazole (VIm) and others. Here, coordinated IL monomers are formed comprising a variety of alkali and alkaline earth Mn+(Tf2N−)n salts with MMA and VIm, and polymerization behavior is investigated using real‐time attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT‐IR). The size and valency of the coordinated Mn+ cations are observed to strongly influence monomer reactivity, as evidenced by significant changes in FT‐IR spectra. This work further demonstrates that the bulky, delocalized [Tf2N]− anion allows for facile introduction of a wide range of Mn+ cations into organic polymers without phase separation, which may open opportunities for new composite materials.
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Anhidrosis in multiple system atrophy involves pre‐ and postganglionic sudomotor dysfunction
Authors: Coon Elizabeth A. , Fealey Robert D. , Sletten David M. , Mandrekar Jay N. , Benarroch Eduardo E. , Sandroni Paola , Low Phillip A. , Singer Wolfgang 
Published: November 2016

AbstractBackgroundThe objective of this study was to characterize the degree, pattern, lesion site, and temporal evolution of sudomotor dysfunction in multiple system atrophy (MSA) and to evaluate differences by parkinsonian (MSA‐parkinsonism) and cerebellar (MSA‐cerebellar) subtypes.MethodsAll cases of MSA evaluated at Mayo Clinic Rochester between 2005 and 2010 with postganglionic sudomotor testing and thermoregulatory sweat test were reviewed. Pattern and lesion site (preganglionic, postganglionic, or mixed) were determined based on thermoregulatory sweat test and postganglionic sudomotor testing.ResultsThe majority of the 232 patients were MSA‐parkinsonism (145, 63%). Initial postganglionic sudomotor testing was abnormal in 59%, whereas thermoregulatory sweat test was abnormal in 95% of all patients. MSA‐parkinsonism patients were more likely to have an abnormal thermoregulatory sweat test compared with MSA‐cerebellar (98% versus 90%, P = 0.006) and had a higher mean percentage of anhidrosis (57%) compared with MSA‐cerebellar (48%; P = 0.033). Common anhidrosis patterns were regional (38%) and global (35%). The site of the lesion was preganglionic in 47% and mixed (preganglionic and postganglionic) in 41%. The increase in anhidrosis per year was 6.2% based on 70 repeat thermoregulatory sweat tests performed on 29 patients. The frequency of postganglionic sudomotor abnormalities increased over time.ConclusionsOur findings suggest: (1) sudomotor dysfunction is almost invariably present in MSA and even more common and severe in MSA‐parkinsonism than MSA‐cerebellar; (2) a preganglionic pattern of sweat loss is common in MSA; however, pre‐ and postganglionic abnormalities may coexist; and (3) the increasing frequency of postganglionic sudomotor dysfunction over time suggests involvement of postganglionic fibers or sweat glands later in the disease course. © 2016 International Parkinson and Movement Disorder Society