bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herpesvirus-Associated Lymphadenitis Distorts Fibroblastic Reticular Cell Microarchitecture and Attenuates CD8 T Cell Responses to Neurotropic Infection in Mice Lacking the STING-IFNα/β Defense Pathways
Authors: Royer Derek J. , Conrady Christopher D. , Carr Daniel J. J. 
Published: August 2016

Abstract
Type I IFN (IFN-α/β)–driven immune responses to acute viral infection are critical to counter replication and prevent dissemination. However, the mechanisms underlying host resistance to HSV type 1 (HSV-1) are incompletely understood. In this study, we show that mice with deficiencies in IFN-α/β signaling or stimulator of IFN genes (STING) exhibit exacerbated neurovirulence and atypical lymphotropic dissemination of HSV-1 following ocular infection. Synergy between IFN-α/β signaling and efficacy of early adaptive immune responses to HSV-1 were dissected using bone marrow chimeras and adoptive cell transfer approaches to profile clonal expansion, effector function, and recruitment of HSV-specific CD8+ T cells. Lymphotropic viral dissemination was commensurate with abrogated CD8+ T cell responses and pathological alterations of fibroblastic reticular cell networks in the draining lymph nodes. Our results show that resistance to HSV-1 in the trigeminal ganglia during acute infection is conferred in part by STING and IFN-α/β signaling in both bone marrow–derived and –resident cells, which coalesce to support a robust HSV-1–specific CD8+ T cell response.
A Narrative Review and Novel Framework for Application of Team-Based Learning in Graduate Medical Education
Authors: Poeppelman Rachel Stork , Liebert Cara A. , Vegas Daniel Brandt , Germann Carl A. , Volerman Anna 
Published: August 2016

ABSTRACT

Background
Team-based learning (TBL) promotes problem solving and teamwork, and has been applied as an instructional method in undergraduate medical education with purported benefits. Although TBL curricula have been implemented for residents, no published systematic reviews or guidelines exist for the development and use of TBL in graduate medical education (GME).

Objective
To review TBL curricula in GME, identify gaps in the literature, and synthesize a framework to guide the development of TBL curricula at the GME level.

Methods
We searched PubMed, MEDLINE, and ERIC databases from 1990 to 2014 for relevant articles. References were reviewed to identify additional studies. The inclusion criteria were peer-reviewed publications in English that described TBL curriculum implementation in GME. Data were systematically abstracted and reviewed for consensus. Based on included publications, a 4-element framework—system, residents, significance, and scaffolding—was developed to serve as a step-wise guide to planning a TBL curriculum in GME.

Results
Nine publications describing 7 unique TBL curricula in residency met inclusion criteria. Outcomes included feasibility, satisfaction, clinical behavior, teamwork, and knowledge application.

Conclusions
TBL appears feasible in the GME environment, with learner reactions ranging from positive to neutral. Gaps in the literature occur within each of the 4 elements of the suggested framework, including: system, faculty preparation time and minimum length of effective TBL sessions; residents, impact of team heterogeneity and inconsistent attendance; significance, comparison to other instructional methods and outcomes measuring knowledge retention, knowledge application, and skill development; and scaffolding, factors that influence the completion of preparatory work.
Kaliabu dan Salaman, Ketika Internet Telah Menjamahmu: Cerita Tentang Desainer Kampung di Indonesia
Authors: Rina Ratna Cahaya 
Published: August 2016

Kaliabu and Salaman, When the Internet Touches You: The Story of Indonesia’s Village DesignersKaliabu is a remote village in the district of Salaman, far from the center of the developing Indonesian economy, that has been making appearances in Indonesian media. The region has hundreds of graphic designers who work online to create logos, picked up through design contests organized by the online design agency 99designs. While there is no doubt that learning design skills can be one way to improve socio-economic conditions in remote communities, there are many questions about the way this platform is changing the nature of the design industry. Kaliabu, sebuah desa terpencil di Kecamatan Salaman menjadi terkenal dengan sebutan Kampung setelah diliput media. Disebut demikian, karena wilayah ini memiliki ratusan desainer grafis yang bekerja secara online untuk membuat desain logo melalui kontes-kontes desain yang diselenggarakan oleh 99Designs. Hal serupa juga terjadi di Kota Kecamatan Salaman. Namun, setelah usai liputan media, tidak semua desainer melihat cara ini sebagai satu-satunya jalan untuk memperbaiki hidup. Namun tidak dipungkiri bahwa desain menjadi salah satu cara yang dapat memperbaiki sosial ekonomi masyarakat terpencil yang jauh dari pusat perekonomian Indonesia.
Characteristics of total gaseous mercury (TGM) concentrations in an
industrial complex in South Korea: impacts from local sources
Authors: Seo Yong-Seok , Jeong Seung-Pyo , Holsen Thomas M. , Han Young-Ji , Choi Eunhwa , Park Eun Ha , Kim Tae Young , Eum Hee-Sang , Park Dae Gun , Kim Eunhye , Kim Soontae , Kim Jeong-Hun , Choi Jaewon , Yi Seung-Muk 
Published: August 2016

Abstract. Total gaseous mercury (TGM) concentrations were measured every 5 min in Pohang, Gyeongsangbuk-do, Korea, during summer (17–23 August 2012), fall (9–17 October 2012), winter (22–29 January 2013), and spring (26 March–3 April 2013) to (1) characterize the hourly and seasonal variations of atmospheric TGM concentrations; (2) identify the relationships between TGM and co-pollutants; and (3) identify likely source directions and locations of TGM using the conditional probability function (CPF), conditional bivariate probability function (CBPF) and total potential source contribution function (TPSCF). The TGM concentration was statistically significantly highest in fall (6.7 ± 6.4 ng m−3), followed by spring (4.8 ± 4.0 ng m−3), winter (4.5 ± 3.2 ng m−3) and summer (3.8 ± 3.9 ng m−3). There was a weak but statistically significant negative correlation between the TGM concentration and ambient air temperature (r = −0.08, p<0.05). Although the daytime temperature (14.7 ± 10.0 °C) was statistically significantly higher than that in the nighttime (13.0 ± 9.8 °C) (p<0.05), the daytime TGM concentration (5.3 ± 4.7 ng m−3) was statistically significantly higher than that in the nighttime (4.7 ± 4.7 ng m−3) (p<0.01), possibly due to local emissions related to industrial activities and activation of local surface emission sources. The observed ΔTGM ∕ ΔCO was significantly lower than that of Asian long-range transport, but similar to that of local sources in Korea and in US industrial events, suggesting that local sources are more important than those of long-range transport. CPF, CBPF and TPSCF indicated that the main sources of TGM were iron and manufacturing facilities, the hazardous waste incinerators and the coastal areas.
Massive asphalt deposits, oil seepage, and gas venting support abundant
chemosynthetic communities at the Campeche Knolls, southern Gulf of Mexico
Authors: Sahling Heiko , Borowski Christian , Escobar-Briones Elva , Gaytán-Caballero Adriana , Hsu Chieh-Wei , Loher Markus , MacDonald Ian , Marcon Yann , Pape Thomas , Römer Miriam , Rubin-Blum Maxim , Schubotz Florence , Smrzka Daniel , Wegener Gunter , Bohrmann Gerhard 
Published: August 2016

Abstract. Hydrocarbon seepage is a widespread process at the continental margins of the Gulf of Mexico. We used a multidisciplinary approach, including multibeam mapping and visual seafloor observations with different underwater vehicles to study the extent and character of complex hydrocarbon seepage in the Bay of Campeche, southern Gulf of Mexico. Our observations showed that seafloor asphalt deposits previously only known from the Chapopote Knoll also occur at numerous other knolls and ridges in water depths from 1230 to 3150 m. In particular the deeper sites (Chapopopte and Mictlan knolls) were characterized by asphalt deposits accompanied by extrusion of liquid oil in form of whips or sheets, and in some places (Tsanyao Yang, Mictlan, and Chapopote knolls) by gas emission and the presence of gas hydrates in addition. Molecular and stable carbon isotopic compositions of gaseous hydrocarbons suggest their primarily thermogenic origin. Relatively fresh asphalt structures were settled by chemosynthetic communities including bacterial mats and vestimentiferan tube worms, whereas older flows appeared largely inert and devoid of corals and anemones at the deep sites. The gas hydrates at Tsanyao Yang and Mictlan Knolls were covered by a 5-to-10 cm-thick reaction zone composed of authigenic carbonates, detritus, and microbial mats, and were densely colonized by 1–2 m-long tube worms, bivalves, snails, and shrimps. This study increased knowledge on the occurrences and dimensions of asphalt fields and associated gas hydrates at the Campeche Knolls. The extent of all discovered seepage structure areas indicates that emission of complex hydrocarbons is a widespread, thus important feature of the southern Gulf of Mexico.
A 1/16° eddying simulation of the global NEMO sea-ice–ocean system
Authors: Iovino Doroteaciro , Masina Simona , Storto Andrea , Cipollone Andrea , Stepanov Vladimir N. 
Published: August 2016

Abstract. Analysis of a global eddy-resolving simulation using the NEMO general circulation model is presented. The model has 1/16° horizontal spacing at the Equator, employs two displaced poles in the Northern Hemisphere, and uses 98 vertical levels. The simulation was spun up from rest and integrated for 11 model years, using ERA-Interim reanalysis as surface forcing. Primary intent of this hindcast is to test how the model represents upper ocean characteristics and sea ice properties. Analysis of the zonal averaged temperature and salinity, and the mixed layer depth indicate that the model average state is in good agreement with observed fields and that the model successfully represents the variability in the upper ocean and at intermediate depths. Comparisons against observational estimates of mass transports through key straits indicate that most aspects of the model circulation are realistic. As expected, the simulation exhibits turbulent behaviour and the spatial distribution of the sea surface height (SSH) variability from the model is close to the observed pattern. The distribution and volume of the sea ice are, to a large extent, comparable to observed values. Compared with a corresponding eddy-permitting configuration, the performance of the model is significantly improved: reduced temperature and salinity biases, in particular at intermediate depths, improved mass and heat transports, better representation of fluxes through narrow and shallow straits, and increased global-mean eddy kinetic energy (by ∼ 40 %). However, relatively minor weaknesses still exist such as a lower than observed magnitude of the SSH variability. We conclude that the model output is suitable for broader analysis to better understand upper ocean dynamics and ocean variability at global scales. This simulation represents a major step forward in the global ocean modelling at the Euro-Mediterranean Centre on Climate Change and constitutes the groundwork for future applications to short-range ocean forecasting.
GAS-PHASE POLYMERIZATION OF 1,3-BUTADIENE WITH PS-DVB–SUPPORTED RARE EARTH CATALYST AND AN INVESTIGATION ON EFFECTS OF DISPERSANTS
Authors: Hong Kan , Ni Xufeng , Shen Zhiquan 
Published: August 2016

ABSTRACT
Organic supporter poly(styrene-co-divinylbenzene) (PS-DVB) was synthesized, and gas-phase polymerization of 1,3-butadiene was performed with PS-DVB–supported Nd(vers)3/Al2Et3Cl3/Al(i-Bu)3 catalysts. Millimeter-sized granules of high cis-1,4 content (&gt;98%) polybutadiene with an Mn of 5.3–5.5 × 105 were obtained. Dispersants used in gas-phase polymerization of 1,3-butadiene have a significant influence on apparent activities of catalysts and appearance of products. The morphology of polybutadiene granules was also investigated by scanning electron microscopy.
Retinal and Choroidal Thicknesses Measured with Swept-Source Optical Coherence Tomography after Surgery for Idiopathic Macular Hole
Authors: Bardak Handan , Gunay Murat , Bardak Yavuz , Ercalik Yesim 
Published: August 2016

Purpose To evaluate the changes in retinal nerve fiber layer thickness (RNFLT), ganglion cell layer thickness (GCLT), retinal thickness (RT), and subfoveal choroidal thickness (SFCT) in eyes that received pars plana vitrectomy (PPV) with internal limiting membrane (ILM) peeling for idiopathic macular hole (IMH) and to compare these data with data from fellow eyes and healthy controls. Methods This cross-sectional study included 49 subjects. Eighteen eyes that underwent surgery for IMH, 18 fellow eyes, and 31 eyes of the healthy controls were designated as group 1, group 2, and group 3, respectively. The RNFLT, GCLT, RT, and SFCT were measured by using the swept-source optical coherence tomography (SS-OCT) device at last postoperative visit. Results The RNFLT was significantly lower in group 1 than group 2 and group 3 (p<0.05). The GCLT was significantly reduced in all sectors in group 1 as compared to group 2 and group 3 (p<0.05). The RT was found to be significantly lower (except in central field) in group 1 than group 2 and group 3 (p<0.05). The SFCT was significantly decreased in group 1 as compared to group 2 and group 3 (p<0.05). Conclusions A reduction in the RNFLT, GCLT, RT, and SFCT was observed following PPV with ILM peeling for IMH detected by SS-OCT.
Effect of Extremely Low Frequency Electromagnetic Field on MAP2 and Nestin Gene Expression of Hair Follicle Dermal Papilla Cells
Authors: Moraveji Marzie , Haghighipour Nooshin , Keshvari Hamid , Abbariki Tannaz Nourizadeh , Shokrgozar Mohammad Ali , Amanzadeh Amir 
Published: August 2016

Introduction In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation. Aim The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs). Methods In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied. To assess the effect of extended test time on the expression of neural differentiation markers (Nestin and MAP2), an EMF group was prepared and treated for a period of 14 consecutive days. The beneficial role of EMF in inducing neural differentiation was shown by real-time PCR analysis. Results The higher expression of MAP2 after 14 days compared to that after 5 days and decrease of cell proliferation on days 5 to 20 were indicative of the positive effect of extending treatment time on neural differentiation by evaluation of gene expression in EMF group.
Use of Autologous Epithelium Transplantation on Various Scaffolds to Cover Tissue Loss in Oral Cavity: Long-Term Observation
Authors: Orzechowska-Wylęgała Bogusława , Dobrowolski Dariusz , Puzzolo Domenico , Wowra Bogumił , Niemiec Wiktor , Wylęgała Adam , Szczubiałka Krzysztof 
Published: August 2016

Background The aim of this study was to investigate the application of mucous membrane keratinocyte cultures on amniotic membrane and on poly(L-lactic acid) (PLLA) Purasorb PL38 to cover tissue loss in the oral cavity. Developments in molecular biology techniques and tissue engineering allow the culturing and identification of cells that can be anchored in the wound to achieve integrity of the tissue. Transplantation of tissues obtained from the patient's own cells is superior to allogenous transplantation where there is a possibility of transfection, rejection and the need for long-term immunosuppression. Methods In 9 patients (15 procedures) keratinocytes cultured on amniotic membrane and PLLA were transplanted to cover antro-oral fistulas and bone loss after osteoradionecrosis. Results In all 6 patients with outlasting antro-oral fistulas, the defects were healed. In 3 patients with 5 cases of tissue loss after osteoradionecrosis, we obtained healing of the wound in only 1 case. Histological examination of the cultures indicated that cultured cells formed well-differentiated layers, very similar to the keratinocytes of mucous membranes, although those seeded on amniotic membrane formed a single layer of cells, while those seeded on the PLLA scaffold were arranged on 2 or more layers: these differences were shown to be statistically significant with a morphometric analysis. Conclusions Autologous transplants of epithelium cultured on amniotic membrane and PLLA constitute a new and effective way of covering nonhealing tissue loss in the oral cavity in chosen cases, using modern methods of tissue engineering.
Preoperative Measurement of Serum C-Reactive Protein: Is it Useful in the Differential Diagnosis of Adnexal Masses?
Authors: Yoshida Adriana , Derchain Sophie F. , Pitta Denise R. , Crozatti Nathália , Andrade Liliana A.L.A. , da Silva Rodrigo F. , Sarian Luis O. 
Published: August 2016

Background Serum biomarkers may help to discriminate malignant from benign adnexal masses with equivocal features on imaging. Adequate discrimination of such tumors is crucial for referring patients to either a specialized cancer center or a nonspecialized gynecology service. Aim We aimed to investigate whether the preoperative level of serum C-reactive protein (CRP), alone or combined with CA125 and menopausal status in the Ovarian Score (OVS), is useful in the prediction of malignancy in women with ovarian tumors. Methods This cross-sectional study included 293 patients who underwent surgery in a tertiary cancer center. Receiver operating characteristic (ROC) areas under the curves (AUC) for CRP, CA125 and OVS were calculated in different scenarios, as well as their sensitivity and specificity, using standard cutoff points (for CRP, 10 mg/L; for CA125, 35 U/mL). Results CA125 and the OVS performed significantly better than CRP alone in the differentiation of benign disease from epithelial ovarian cancer (EOC) (AUC = 0.86 for CA125, 0.79 for OVS, and 0.73 for CRP). OVS and CRP alone were superior to CA125 only in the differentiation of borderline ovarian tumors from advanced stages of EOC and non-EOC. Sensitivity and specificity were 52.5% and 83%, respectively, for CRP, 77.9% and 66.7% for CA125, and 71.3% and 67.8% for OVS. Conclusions OVS is as good as CA125 in the differentiation of benign tumors from ovarian cancer. The addition of CA125 and menopausal status to CRP enhanced the relatively low discriminatory power of isolated CRP.
Health Economic Burden of Deep Infiltrating Endometriosis Surgically Treated in a Referral Center
Authors: Koltermann Katharina C. , Thiel-Moder Ulrike , Willich Stefan N. , Reinhold Thomas , Ebert Andreas D. 
Published: August 2016

Purpose This retrospective study aimed to describe patient characteristics of women with deep infiltrating endometriosis [DIE] affecting the bowel and bladder treated in a referral center in Germany, and to calculate direct costs during inpatient stay for surgery. Methods Patient characteristics and cost-relevant factors of women with DIE of the bladder or bowel surgically treated during 2008-2012 in a referral center were gathered from medical records. Patient economic data were retrieved from the hospital's finance and accounts department. Results One hundred and forty-eight cases with clinical information and cost data were analyzed retrospectively. Most cases had bowel endometriosis (91%) and were classified as severe (66%). The women analyzed were in their reproductive peak years (34 years; SD 6.3 years), of normal weight (body mass index [BMI] 23; SD 4.0) and most of them were employed (80%). Nearly all women (95%) were affected by multiple symptoms at hospital admission and the majority had a prior diagnosis of endometriosis (76%). Mean total costs per case were €11,458 (95% CI: €10,589-€12,326). Forty-four percent of the total costs were mean staff costs (€5,039), 30% were mean infrastructure costs (€3,425) and 26% mean material costs (€2,993). Conclusions This study is the first to estimate the surgery-related direct costs of DIE in Germany from a hospital's perspective based on data from a specialized center. Resource utilization and total costs of DIE are substantial.
Tegaderm™ CHG Dressing Significantly Improves Catheter-related Infection Rate in Hemodialysis Patients
Authors: Righetti Marco , Palmieri Nicola , Bracchi Oscar , Prencipe Mario , Bruschetta Elena , Colombo Francesca , Brenna Irene , Stefani Francesca , Amar Karen , Scalia Alfio , Conte Ferruccio 
Published: August 2016

Introduction Catheter-related infections are an important clinical problem in maintenance hemodialysis patients. Catheter-related bloodstream infections have a negative effect on survival, hospitalization and cost of care. Tegaderm™ chlorhexidine gluconate (CHG) dressing may be useful to reduce catheter-related infection rates. Methods We performed a study to assess the efficacy of Tegaderm™ CHG dressing for reducing catheter-related infections. We designed a prospective randomized cross-over study with a scheme of two treatments, Tegaderm™ CHG dressing versus standard dressing, and two periods of six months. Catheter-related infection rate was the primary outcome. We enrolled 59 prevalent hemodialysis patients. Results Catheter-related infection rate per 1000 catheter days was reduced from 1.21 in patients using standard dressing to 0.28 in patients with Tegaderm™ CHG dressing (p = 0.02). Catheter-related bloodstream infection rate per 1000 catheter days was equal to 0.09 in patients with Tegaderm™ CHG dressing versus 0.65 in patients with standard dressing (p = 0.05). Annual total healthcare costs for catheter-related bloodstream infections were estimated equal to EUR62,459 versus EUR300,399, respectively, for patients with Tegaderm™ CHG versus standard dressing. Conclusions This is the first prospective study to show that Tegaderm™ CHG dressing significantly reduces catheter-related infection rates in hemodialysis patients.