bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning, Characterization, and Mapping of a Murine Promiscuous Chemokine Receptor Gene: Homolog of the Human Duffy Gene
Authors: Luo Hong , Chaudhuri Asok , Johnson Kenneth R. , Neote Kuldeep , Zbrzezna Valerie , He Yu , Pogo A. Oscar 
Published: May 2017

We report here the isolation and genomic organization of the orthologous mouse Duffy gene, named Dfy. It is a single copy gene located in chromosome 1 in a region homologous to the human Duffy gene (FY). Sequence analyses indicate that Dfyconsists of two exons: exon 1 of 55 nucleotides, which encodes 7 amino acid residues; and exon 2 of 1038 nucleotides, which encodes 327 residues. The single intron consists of 462 nucleotides. The 5′-end promoter region contains motifs involved in vertebrate development in addition to potential binding sites of factors for globin transcription. The open reading frame (ORF) shows 60% homology with the human Duffy protein. However, mouse erythrocytes are serologically Duffy-negative and mouse erythrocyte membrane proteins do not cross-react with two Duffy-specific rabbit polyclonal antibodies. The deduced protein predicts a Mr of 36,692 and carries three potential N-glycosylation sites to asparagine residues. Hydropathy analysis predicts an exocellular amino-terminal domain of 57 residues, seven transmembrane α-helices, and an endocellular carboxy-terminal domain of 29 residues. In bone marrow and spleen,Dfy expresses a major 1.4-kb and a minor 1.8-kb mRNA. Contrary to humans, Dfy is expressed in liver, synthesizing a 1.4-kb mRNA, and is repressed in kidney. Dfy is highly expressed in mouse brain and produces a major 8.5-kb and a minor 10.2-kb mRNA. The human erythroleukemia K562 cells, transfected with cDNA encoding the mouse Duffy-like protein and mouse erythrocytes, have the same chemokine binding profiles indicating that they contain the same protein.[The sequence data described in this paper have been submitted to GenBank under accession nos. AF016584 and AF016697.]
Contig Maps and Genomic Sequencing Identify Candidate Genes in the Usher 1C Locus
Authors: Higgins Michael J. , Day Colleen D. , Smilinich Nancy J. , Ni L. , Cooper Paul R. , Nowak Norma J. , Davies Chris , de Jong Pieter J. , Hejtmancik Fielding , Evans Glen A. , Smith Richard J.H. , Shows Thomas B. 
Published: May 2017

Usher syndrome 1C (USH1C) is a congenital condition manifesting profound hearing loss, the absence of vestibular function, and eventual retinal degeneration. The USH1C locus has been mapped genetically to a 2- to 3-cM interval in 11p14–15.1 between D11S899 andD11S861. In an effort to identify the USH1C disease gene we have isolated the region between these markers in yeast artificial chromosomes (YACs) using a combination of STS content mapping andAlu–PCR hybridization. The YAC contig is ∼3.5 Mb and has located several other loci within this interval, resulting in the orderCEN-LDHA-SAA1-TPH-D11S1310-(D11S1888/KCNC1)-MYOD1-D11S902D11S921-D11S1890-TEL.Subsequent haplotyping and homozygosity analysis refined the location of the disease gene to a 400-kb interval between D11S902 andD11S1890 with all affected individuals being homozygous for the internal marker D11S921. To facilitate gene identification, the critical region has been converted into P1 artificial chromosome (PAC) clones using sequence-tagged sites (STSs) mapped to the YAC contig, Alu–PCR products generated from the YACs, and PAC end probes. A contig of >50 PAC clones has been assembled between D11S1310 and D11S1890, confirming the order of markers used in haplotyping. Three PAC clones representing nearly two-thirds of the USH1C critical region have been sequenced. PowerBLAST analysis identified six clusters of expressed sequence tags (ESTs), two known genes (BIR,SUR1) mapped previously to this region, and a previously characterized but unmapped gene NEFA(DNA binding/EF hand/acidic amino-acid-rich). GRAIL analysis identified 11 CpG islands and 73 exons of excellent quality. These data allowed the construction of a transcription map for the USH1C critical region, consisting of three known genes and six or more novel transcripts. Based on their map location, these loci represent candidate disease loci for USH1C. The NEFA gene was assessed as the USH1C locus by the sequencing of an amplified NEFA cDNA from an USH1C patient; however, no mutations were detected.[The sequence data described in this paper have been submitted to GenBank under accession numbersAC000406–AC000407.]
Homologs of the Yeast Longevity Gene LAG1 in Caenorhabditis elegans and Human
Authors: Jiang James C. , Kirchman Paul A. , Zagulski Marek , Hunt Jay , Jazwinski S. Michal 
Published: May 2017

LAG1 is a longevity gene, the first such gene to be identified and cloned from the yeast Saccharomyces cerevisiae. A close homolog of this gene, which we call LAC1, has been found in the yeast genome. We have cloned the human homolog ofLAG1 with the ultimate goal of examining its possible function in human aging. In the process, we have also cloned a homolog from the nematode worm Caenorhabditis elegans. Both of these homologs,LAG1Hs and LAG1Ce-1, functionally complemented the lethality of a lag1Δ lac1Δdouble deletion, despite low overall sequence similarity to the yeast proteins. The proteins shared a short sequence, the Lag1 motif, and a similar transmembrane domain profile. Another, more distant human homolog, TRAM, which lacks this motif, did not complement.LAG1Hs also restored the life span of the double deletion, demonstrating that it functions in establishing the longevity phenotype in yeast. LAG1Hs mapped to 19p12, and it was expressed in only three tissues: brain, skeletal muscle, and testis. This gene possesses a trinucleotide (CTG) repeat within exon 1. This and its expression profile raise the possibility that it may be involved in neurodegenerative disease. This possibility suggests at least one way in which LAG1Hs might be involved in human aging.[The sequence data described in this paper have been submitted to GenBank under accession nos. AF105005–AF105009(LAG1Hs) and AF105010 (LAG1Ce-1).]
Complex β-Satellite Repeat Structures and the Expansion of the Zinc Finger Gene Cluster in 19p12
Authors: Eichler Evan E. , Hoffman Susan M. , Adamson Aaron A. , Gordon Laurie A. , McCready Paula , Lamerdin Jane E. , Mohrenweiser Harvey W. 
Published: May 2017

We investigated the organization, architecture, and evolution of the largest cluster (∼4 Mb) of Krüppel-associated box zinc finger (KRAB–ZNF) genes located in cytogenetic band interval 19p12. A highly integrated physical map (∼700 kb) of overlapping cosmid and BAC clones was developed between genetic STS markers D19S454 and D19S269. Using ZNF91 exon-specific probes to interrogate a detailed EcoRI restriction map of the region, ZNF genes were found to be distributed in a head-to-tail fashion throughout the region with an average density of one ZNF duplicon every 150–180 kb of genomic distance. Sequence analysis of 208,967 bp of this region indicated the presence of two putative ZNF genes: one consisting of a novel member of this gene family (ZNF208) expressed ubiquitously in all tissues examined and the other representing a nonprocessed pseudogene (ZNF209), located 450 kb proximal toZNF208. Large blocks of (∼25-kb) inverted β-satellite repeats with a remarkably symmetrical higher order repeat structure were found to bracket the functional ZNF gene. Hybridization analysis using the β-satellite repeat as a probe indicates that β-satellite interspersion between ZNF gene cassettes is a general property for 1.5 Mb of the ZNF gene cluster in 19p12. Both molecular clock data as well as a retroposon-mapping molecular fossil approach indicate that this ZNF cluster arose early during primate evolution (∼50 million years ago). We propose an evolutionary model in which heteromorphic pericentromeric repeat structures such as the β satellites have been coopted to accommodate rapid expansion of a large gene family over a short period of evolutionary time.[The sequence data described in this paper have been submitted to GenBank under accession nos. AC003973 and AC004004.]
Molecular Basis for the Dominant White Phenotype in the Domestic Pig
Authors: Marklund Stefan , Kijas James , Rodriguez-Martinez Heriberto , Rönnstrand Lars , Funa Keiko , Moller Maria , Lange Dirk , Edfors-Lilja Inger , Andersson Leif 
Published: May 2017

The change of phenotypic traits in domestic animals and crops as a response to selective breeding mimics the much slower evolutionary change in natural populations. Here, we describe that the dominant white phenotype in domestic pigs is caused by two mutations in theKITgene encoding the mast/stem cell growth factor receptor (MGF), one gene duplication associated with a partially dominant phenotype and a splice mutation in one of the copies leading to the fully dominant allele. The splice mutation is a G to A substitution in the first nucleotide of intron 17 and leads to skipping of exon 17. The duplication is most likely a regulatory mutation affecting KIT expression, whereas the splice mutation is expected to cause a receptor with impaired or absent tyrosine kinase activity. Immunocytochemistry showed that this variant form is expressed in 17- to 19-day-old pig embryos. Hundreds of millions of white pigs around the world are assumed to be heterozygous or homozygous for the two mutations.[The EMBL accession numbers for porcineKIT1*0101, KIT1*0202, KIT2*0202,andKIT2*0101areAJ223228–AJ223231, respectively.]
Direct Selection of Conserved cDNAs from the DiGeorge Critical Region: Isolation of a Novel CDC45-Like Gene
Authors: McKie Judith M. , Wadey Roy B. , Sutherland Helen F. , Taylor Catherine L. , Scambler Peter J. 
Published: May 2017

We have used a modified direct selection technique to detect transcripts that are both evolutionary conserved and developmentally expressed. The enrichment for homologous mouse cDNAs by use of human genomic DNA as template is shown to be an efficient and rapid approach for generating transcript maps. Deletions of human 22q11 are associated with several clinical syndromes, with overlapping phenotypes, for example, velocardiofacial syndrome (VCFS) and DiGeorge syndrome (DGS). A large number of transcriptional units exist within the defined critical region, many of which have been identified previously by direct selection. However, no single obvious candidate gene for the VCFS/DGS phenotype has yet been found. Our technique has been applied to the DiGeorge critical region and has resulted in the isolation of a novel candidate gene, Cdc45l2, similar to yeast Cdc45p.[The sequence data described in this paper have been submitted to the EMBL data library under accession nos. AJ0223728 and AF0223729.]
Sequencing of cDNA Clones from the Genetic Map of Tomato (Lycopersicon esculentum)
Authors: Ganal Martin W. , Czihal Rosemarie , Hannappel Ulrich , Kloos Dorothee-U. , Polley Andreas , Ling Hong-Qing 
Published: May 2017

The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions.[cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos.AA824695–AA825005 and the dbEST_Id database under accession nos. 1546519–1546862.]
The most influential researchers in information behaviour
Authors: Soheili Faramarz , Khasseh Ali Akbar , Mousavi-Chelak Afshin 
Published: May 2017

PurposeThe purpose of this paper is to identify the top researchers in information behaviour (IB) based on ideational and social influence indicators.Design/methodology/approachThe population included papers on IB indexed in the Web of Science from 1980 to 2015. UCINET and Bibexcel were the tools used for measuring the ideational and social influence indicators. The correlations among the study variables were measured by applying SPSS and LISREL.FindingsThere was a significant relationship between IB researchers’ productivity and performance, and between ideational influence and social influence. The structural equation modelling showed that a researcher with top placement in his/her co-authorship network can gain higher ideational influence. In total, it seems that the single and traditional criteria are increasingly replacing new and integrative ones in measuring researchers’ scientific influence in fields including IB studies. Results have shown that based on total scores of the studied indicators, Spink, A., Nicholas, D., Ford, N., Huntington, P., Wilson, T.D., and Jamali, H.R. gained the high scores.Originality/valueThe current study used an integrative method based on influence indicators to identify the influential researchers in IB studies. None of the few studies done using bibliometric methods in the realm of IB has investigated the ideational and social influence indicators altogether.
Are Mendeley reader counts high enough for research evaluations when articles are published?
Authors: Thelwall Mike 
Published: May 2017

PurposeMendeley reader counts have been proposed as early indicators for the impact of academic publications. The purpose of this paper is to assess whether there are enough Mendeley readers for research evaluation purposes during the month when an article is first published.Design/methodology/approachAverage Mendeley reader counts were compared to the average Scopus citation counts for 104,520 articles from ten disciplines during the second half of 2016.FindingsArticles attracted, on average, between 0.1 and 0.8 Mendeley readers per article in the month in which they first appeared in Scopus. This is about ten times more than the average Scopus citation count.Research limitations/implicationsOther disciplines may use Mendeley more or less than the ten investigated here. The results are dependent on Scopus’s indexing practices, and Mendeley reader counts can be manipulated and have national and seniority biases.Practical implicationsMendeley reader counts during the month of publication are more powerful than Scopus citations for comparing the average impacts of groups of documents but are not high enough to differentiate between the impacts of typical individual articles.Originality/valueThis is the first multi-disciplinary and systematic analysis of Mendeley reader counts from the publication month of an article.
Disruptive innovation in rural American healthcare: the physician assistant practice
Authors: Kushins Eric R. , Heard Henry , Weber J. Michael 
Published: May 2017

Purpose
This article proposes a new disruptive innovation in healthcare through the development of a physician assistant business model, which can be most readily applied in vulnerable rural health care settings.

Design/methodology/approach
This study reviews the current state of the health care system in terms of physician assistant utilization and primary care shortages in rural communities. The study proposes that the physician assistant-owned and -operated primary care business represents a disruptive innovation, via the application of the five principles of Clayton Christensen’s (1997) thesis on disruptive innovation.

Findings
Considering the current state of the health care industry, the study logically defends the proposed model as a disruptive innovation in that it: focuses on an underserved market, has lower costs, has few competitors, offers high quality and provides a sustainable competitive advantage.

Practical implications
The physician assistant business model is a viable solution for providing primary care for rural communities with educational, financial, transportation and other resource limitations.

Originality/value
This is a unique application of the theory of disruptive innovation, which illustrates how a new business model can solve a chronic shortage in primary care, especially in underserved populations.
Listening to Immokalee moms
Authors: Morton Cynthia , Galindo Sebastian , Morera Maria , Dodoo Naa , Gonzalez Cristobal , Wu Linwan , Fundingsland Suzanne , Auguste Kendra , Headrick Lauren , Monaghan Paul , Shelnutt Karla 
Published: May 2017

Purpose
The purpose of this paper is to present a case study of the research steps taken to identify message directions for a community-wide health communication campaign aimed at supporting healthier eating behaviors among Latino farmworker families in the Immokalee community in Florida. Latino mothers were targeted to understand their beliefs about children’s healthy eating needs and identify communication campaign opportunities that would reinforce choices made in the home, surrounding meal planning, food selection and preparation and portion sizes.

Design/methodology/approach
A sequential mixed-methods approach using focus group, group interview and qualitative message concept testing was used in three distinct stages of research exploration. An independent sample was recruited at each stage through convenience sampling and snowball sampling methods. The stages of change theory provided a framework for research inquiry in the context of healthy eating-at-home practices.

Findings
In general, mothers were aware and participatory in the effort to serve healthy meals for their children at home. The time and money associated with buying nutritious foods and cooking healthy meals daily was stressful, but they were receptive to communication efforts to help them maintain their commitment to give their children healthy foods. These findings provided directional opportunities for the discovery and implementation of health communication strategies. Test results found that the target was most receptive to message strategies that acknowledged the responsibility of both parents to model healthy eating practices to children.

Research limitations/implications
Recruiting resulted in small sample sizes at each stage of the investigation. The reliability of quantitative measurement was a limitation to the research conclusions.

Practical implications
Partnership with the audience from discovery to conceptualization resulted in messages that uniquely resonated with the audience for which they were aimed. Understanding about the target stage of readiness improves message effectiveness to the target group.

Social implications
Health communication campaigns planned and developed with the target audience at its center will generate message strategies that effectively address issues of greatest relevance to those communication agents that seek to change.

Originality/value
The study provides a unique exploration of the steps that health communication agents should pursue to establish a thorough profile of their target audience and the issues that resonate when considering healthy eating behavior at home. The application of the stages of the change model encouraged the discovery of issues surrounding the challenge and highlighted potential obstacles that would mitigate the behavior change efforts.
Influence of the thickness of intumescent paint in the position of steels (fire safety)
Authors: Garcia Harkaitz , Biezma María Victoria , Cuadrado Jesús , Rojí Eduardo 
Published: May 2017

Purpose
The purpose of this paper is to analyze a new structural design applied in industrial frames using two type of steels (S275 and fire resistant (FR)) with different mechanical resistance against fire. To do it, the authors have taken into account variables such as intrinsic metallic design, span length, intumescent paint thickness, and fire time exposure, which offers information about new scenarios of design in industry.

Design/methodology/approach
The key methodology followed has taken into account a modeling program that uses the following variables: 25 and 35 m of span, 45 and 60 fire exposure times, and seven different intumescent paint thickness. An optimum structural design has been evaluated by discretization of each scenario with the particular type of steel, S275 and FR. The obtained approach could be a good guideline for future designs.

Findings
The results and analysis have shown a very good and valid idea of a new structural typology using optimum intumescent paint thickness into the final design of the industrial frame considering that it has two different types of steel. It is in realty a handicap since usually mechanical engineers employ structural steel without paying attention to this new feature.

Practical implications
Cheaper structural designs could be obtained using the two different types of steel considering the proper positioning into the full building.

Originality/value
The validity of design of two types of steel plus intumescent paint in building construction has been shown, and this study will encourage designers to use it, in particular in buildings with high fire risk.
Pushing similarity joins down to the storage layer in XML databases
Authors: Andrade Ribeiro Leonardo , Härder Theo 
Published: May 2017

PurposeThis article aims to explore how to incorporate similarity joins into XML database management systems (XDBMSs). The authors aim to provide seamless and efficient integration of similarity joins on tree-structured data into an XDBMS architecture.Design/methodology/approachThe authors exploit XDBMS-specific features to efficiently generate XML tree representations for similarity matching. In particular, the authors push down a large part of the structural similarity evaluation close to the storage layer.FindingsEmpirical experiments were conducted to measure and compare accuracy, performance and scalability of the tree similarity join using different similarity functions and on the top of different storage models. The results show that the authors’ proposal delivers performance and scalability without hurting the accuracy.Originality/valueSimilarity join is a fundamental operation for data integration. Unfortunately, none of the XDBMS architectures proposed so far provides an efficient support for this operation. Evaluating similarity joins on XML is challenging, because it requires similarity matching on the text and structure. In this work, the authors integrate similarity joins into an XDBMS. To the best of the authors’ knowledge, this work is the first to leverage the storage scheme of an XDBMS to support XML similarity join processing.
Tribological properties of Fe-Cr-B alloy for sliding boot in coal mining machine under dry sliding condition
Authors: Xuan Hongbin , Cui Gongjun 
Published: May 2017

Purpose
To improve the wear resistance of the sliding boot, the wear-resistant Fe-21 Wt.% Cr-5 Wt.% B alloy is prepared, and the wear mechanism is studied under dry sliding condition.

Design/methodology/approach
The anti-wear Fe-21 Wt.% Cr-5 Wt.% B alloy is prepared by powder metallurgy technique. The tribological behavior of Fe-Cr-B alloy sliding against ASTM 1045 steel pin is studied at 30-60 N and 0.03-0.12 m/s using a reciprocating pin-on-disk tribometer under dry sliding condition. Meanwhile, the ASTM 5140 and 3316 steel are studied as compared samples.

Findings
The friction coefficients of tested specimens increase with the increasing normal load. However, this effect is the opposite in case of different sliding speeds. The specific wear rates increase as the sliding speed and normal load increase. The Fe-Cr-B alloy shows the best tribological properties under the dry sliding condition and the wear mechanism is mainly ploughing.

Originality/value
This wear-resistant Fe-21 Wt.% Cr-5 Wt.% B alloy can replace the traditional materials to process the sliding shoes and improve the service life of coal mining machine.