bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and role of inulin in mitigation of enteric sulfur‐containing odor in pigs
Authors: Deng Yuan‐Fan , Liu Yuan‐Yuan , Zhang Yi‐Tao , Wang Yan , Liang Juan‐Boo , Tufarelli Vincenzo , Laudadio Vito , Liao Xin‐Di 
Published: September 2016

AbstractBACKGROUNDThe efficacy and role of inulin in the mitigation of enteric sulfur‐containing odor gases hydrogen sulfide (H2S) and methyl mercaptan (CH3SH) in pigs were examined in this study. Twelve Duroc × Landrace × Yorkshire male finisher pigs (60.7 ± 1.9 kg), housed individually in open‐circuit respiration chambers, were randomly assigned to two dietary groups, namely basal diet (control) and basal diet supplemented with 1% (w/w) inulin. At the end of the 45 day experiment, pigs were slaughtered and volatile fatty acid (VFA) concentration, sulfate radical (SO42−) concentration, population of sulfate‐reducing bacteria (SRB) and expression of methionine gamma‐lyase (MGL) gene were determined in contents from the caecum, colon (two segments) and rectum. Metabonomic analysis was used to compare differences in biochemical composition, and the Illumina MiSeq procedure to investigate differences in bacterial components, in the different parts of the large intestine between inulin‐supplemented and inulin‐free (control) groups.RESULTSInulin decreased (P < 0.05) the average daily enteric H2S and CH3SH production by 12.4 and 12.1% respectively. The concentrations of acetate, propionate and butyrate in the large intestinal content were significantly increased (P < 0.05) with inulin treatment, whereas valerate concentration and MGL mRNA expression decreased (P < 0.05). The growth of Lactobacillus, Butyrivibrio, Pseudobutyrivibrio, Bifidobacterium and Clostridium butyricum was stimulated, while that of Desulfovibrio, the dominant SRB, was inhibited, and there was an accumulation of SO42− in the large intestinal content of the inulin‐supplemented pigs, suggesting that inulin mitigates H2S generation from the SO42− reduction pathway by reducing the growth of SRB.CONCLUSIONThe results showed that inulin mitigates CH3SH generation via three methionine degradation metabolic pathways and H2S generation from two cysteine degradation metabolic pathways, thus resulting in increased synthesis of these two sulfur‐containing amino acids in the pig large intestine. © 2016 Society of Chemical Industry
A meshless method with conforming and nonconforming sub‐domains
Authors: Guo Yong‐Ming , Yagawa Genki 
Published: September 2016

SummaryAn accurate and easy integration technique is desired for the meshless methods of weak form. As is well known, a sub‐domain method is often used in computational mechanics. The conforming sub‐domains, where the sub‐domains are not separated nor overlapped each other, are often used, while the nonconforming sub‐domains could be employed if needed. In the latter cases, the integrations of the sub‐domains may be performed easily by choosing a simple configuration. And then, the meshless method with nonconforming sub‐domains is considered one of the reasonable choices for computational mechanics without the troublesome integration. In this paper, we propose a new sub‐domain meshless method. It is noted that, because the method can employ both the conforming and the nonconforming sub‐domains, the integration for the weak form is necessarily accurate and easy by selecting the nonconforming sub‐domains with simple configuration. The boundary value problems including the Poisson's equation and the Helmholtz's equation are analyzed by using the proposed method. The numerical solutions are compared with the exact solutions and the solutions of the collocation method, showing that the relative errors by using the proposed method are smaller than those by using the collocation method and that the proposed method possesses a good convergence. Copyright © 2016 John Wiley & Sons, Ltd.
Robust isophase margin control of oscillatory systems with large uncertainties in their parameters: A fractional‐order control approach
Authors: Feliu‐Batlle V. 
Published: September 2016

SummaryThis paper addresses the problem of designing controllers that are robust to large changes in the undamped natural frequencies of a plant. Plants must be represented by means of minimum phase rational transfer functions of an arbitrary order whose oscillatory dynamics must fulfill the pole‐zero interlacing property on the imaginary axis. The design specifications are as follows: (i) a phase margin for the nominal plant; (ii) a gain crossover frequency for the nominal plant; and (iii) a constant phase margin for large variations in the undamped natural frequencies of the plant, the zeros associated with the oscillatory part of the plant and the gain of the plant. Theorems are proposed that define the structure of the controllers that fulfill these specifications. We show that these robust controllers must necessarily include a fractional‐order integro‐differential term. Analytical simple expressions with which to obtain the simplest controllers that verify the aforementioned specifications are also obtained. Some relevant features of these fractional‐order controllers are later highlighted. Finally, as an example, these controllers are applied to a Buck electronic converter. Copyright © 2016 John Wiley & Sons, Ltd.
Dorsalis Pedis Artery-based Flap to Cover Nonhealing Wounds over the Tendo Achillis—A Case Series
Authors: Varun Chandra , Kumar Singh Raj 
Published: September 2016

Background/Purpose Soft tissue necrosis after the repair of a ruptured tendo Achillis is a difficult problem commonly encountered by orthopaedic surgeons. Such wounds are difficult to manage because of the tenuous blood supply and the characteristic anatomical features of the area. Flaps such as the reverse sural flap, medial plantar flap, gastrocnemius flap and free flaps cover such wounds with a good success rate. The aim of our study was to investigate the use of a dorsalis pedis artery (DPA)-based flap to cover a wound over the tendo Achillis insertion area. Methods We report a series of 11 patients in whom wounds over the tendo Achillis insertion area were covered with a DPA-based flap. Between 1995 and 2012, 11 patients (nine men and two women), who were referred to us with a nonhealing wound over the tendo Achillis because of soft tissue necrosis after the open repair of a ruptured tendon, were selected for soft tissue coverage of the wound by using a DPA-based flap. Results The average follow-up period was 2.4 years (range, 1–6 years). The mean operative time was 1.7 hours (range, 1.5–2.0 hours). At the latest follow-up examination, the flaps had survived in all patients. Conclusion A DPA-based flap is a good and relatively less complex procedure to use to cover defects over the tendo Achillis with minimum donor site morbidity.
Associations between social vulnerabilities and dietary patterns in European children: the Identification and prevention of Dietary- and lifestyle-induced health EFfects In Children and infantS (IDEFICS) study
Authors: Iguacel Isabel , Fernández-Alvira Juan M. , Bammann Karin , De Clercq Bart , Eiben Gabriele , Gwozdz Wencke , Molnar Dénes , Pala Valeria , Papoutsou Stalo , Russo Paola , Veidebaum Toomas , Wolters Maike , Börnhorst Claudia , Moreno Luis A. 
Published: September 2016

AbstractSocio-economic inequalities in childhood can determine dietary patterns, and therefore future health. This study aimed to explore associations between social vulnerabilities and dietary patterns assessed at two time points, and to investigate the association between accumulation of vulnerabilities and dietary patterns. A total of 9301 children aged 2–9 years participated at baseline and 2-year follow-up examinations of the Identification and prevention of Dietary- and lifestyle-induced health EFfects In Children and infantS study. In all, three dietary patterns were identified at baseline and follow-up by applying the K-means clustering algorithm based on a higher frequency of consumption of snacks and fast food (processed), sweet foods and drinks (sweet), and fruits and vegetables (healthy). Vulnerable groups were defined at baseline as follows: children whose parents lacked a social network, children from single-parent families, children of migrant origin and children with unemployed parents. Multinomial mixed models were used to assess the associations between social vulnerabilities and children’s dietary patterns at baseline and follow-up. Children whose parents lacked a social network (OR 1·31; 99 % CI 1·01, 1·70) and migrants (OR 1·45; 99 % CI 1·15, 1·83) were more likely to be in the processed cluster at baseline and follow-up. Children whose parents were homemakers (OR 0·74; 99 % CI 0·60, 0·92) were less likely to be in the processed cluster at baseline. A higher number of vulnerabilities was associated with a higher probability of children being in the processed cluster (OR 1·78; 99 % CI 1·21, 2·62). Therefore, special attention should be paid to children of vulnerable groups as they present unhealthier dietary patterns.
Land inequality and deforestation in the Brazilian Amazon
Authors: Sant'anna André Albuquerque 
Published: September 2016

AbstractThis paper investigates the relationship between land concentration and deforestation in the Brazilian Amazon. It develops a conceptual framework in which an individual may have three alternatives: to be a farmer in an already established place, to be a rural worker, or to migrate to the agricultural frontier in order to deforest. This model implies that land inequality affects deforestation positively. Based on data from municipalities with positive deforestation from 2002 to 2011, a model has been estimated to test this theoretical prediction. By making use of an instrumental variable, results show that there is statistical evidence to support the existence of a direct relationship between land inequality and deforestation. Results are stronger for the period 2002–2005. This might be due to command and control policies which have significantly increased the cost of clearing land since the mid-2000s.
Narkolepsie im Kindes- und Jugendalter: Symptome, Diagnostik und Therapie. Ein Fallbericht
Authors: Gehrmann Jochen , Siegler Dominik , Ignacy Evelin , Reimer Inga 
Published: September 2016

Zusammenfassung. Narkolepsie ist eine seltene, multifaktoriell bedingte Erkrankung des Hypothalamus mit den Leitsymptomen Tagesschläfrigkeit und Kataplexie. (Schlaf-)EEG und HLA-DR-Genotyp sichern die Diagnose. Wir berichten hier über ein 14-jähriges Mädchen mit Ängsten, Depressivität, Schulvermeidung, sozialem Rückzug sowie sehr häufigen imperativen Schlafanfällen tagsüber mit Kataplexie. Im Kindes- und Jugendalter gibt es gegenwärtig für die Indikation Narkolepsie kein zugelassenes Medikament. Unsere Patientin profitierte signifikant und zügig von einer off-label medikamentösen Therapie mit dem Wirkstoff Methylphenidat in Verbindung mit Psychoedukation, Verhaltens- und Familientherapie. Narkolepsie ist eine sehr seltene, aber möglicherweise unterschätzte Differentialdiagnose bei unklarer Tagesmüdigkeit und Ängsten bzw. Depressivität im Kindes- und Jugendalter. Die Kern- und komorbiden Symptome sprachen in diesem Fall gut auf eine allerdings höher dosierte Stimulanzientherapie an
A global experimental dataset for assessing grain legume production
Authors: Cernay Charles , Pelzer Elise , Makowski David 
Published: September 2016

AbstractGrain legume crops are a significant component of the human diet and animal feed and have an important role in the environment, but the global diversity of agricultural legume species is currently underexploited. Experimental assessments of grain legume performances are required, to identify potential species with high yields. Here, we introduce a dataset including results of field experiments published in 173 articles. The selected experiments were carried out over five continents on 39 grain legume species. The dataset includes measurements of grain yield, aerial biomass, crop nitrogen content, residual soil nitrogen content and water use. When available, yields for cereals and oilseeds grown after grain legumes in the crop sequence are also included. The dataset is arranged into a relational database with nine structured tables and 198 standardized attributes. Tillage, fertilization, pest and irrigation management are systematically recorded for each of the 8,581 crop*field site*growing season*treatment combinations. The dataset is freely reusable and easy to update. We anticipate that it will provide valuable information for assessing grain legume production worldwide.
A recurrent deletion in the SLC5A2 gene including the intron 7 branch site responsible for familial renal glucosuria
Authors: Zhao Xiangzhong , Cui Li , Lang Yanhua , liu Ting , Lu Jingru , Wang Cui , Tuffery-Giraud Sylvie , Bottillo Irene , Wang Xinsheng , Shao Leping 
Published: September 2016

AbstractFamilial renal glycosuria (FRG) is caused by mutations in the SLC5A2 gene, which codes for Na+-glucose co-transporters 2 (SGLT2). The aim of this study was to analyze and identify the mutations in 16 patients from 8 families with FRG. All coding regions, including intron-exon boundaries, were analyzed using PCR followed by direct sequence analysis. Six mutations in SLC5A2 gene were identified, including five missense mutations (c.393G > C, p.K131N; c.1003A > G, p.S335G; c.1343A > G, p.Q448R; c.1420G > C, p.A474P; c.1739G > A, p.G580D) and a 22-bp deletion in intron 7 (c.886(-10_-31)del) removing the putative branch point sequence. By the minigene studies using the pSPL3 plasmids, we confirmed that the deletion c.886(-10_-31)del acts as a splicing mutation. Furthermore, we found that this deletion causes exclusion of exon 8 in the SCL5A2 transcript in patients. The mutation c.886(-10_-31)del was present in 5 (62.5%) of 8 families and accounts for about 37.5% of the total alleles (6/16). In conclusion, six mutations resulting in FRG were found and the c.886(-10_-31)del may be the high frequency mutation that can be screened in FRG patients with uniallelic or negative SLC5A2 mutations.
AcCNET (

Ac

cessory Genome

C

onstellation

Net

work): comparative genomics software for accessory genome analysis using bipartite networks
Authors: Lanza Val F , Baquero Fernando , de la Cruz Fernando , Coque Teresa M 
Published: September 2016

Abstract
AcCNET (Accessory genome Constellation Network) is a Perl application that aims to compare accessory genomes of a large number of genomic units, both at qualitative and quantitative levels. Using the proteomes extracted from the analysed genomes, AcCNET creates a bipartite network compatible with standard network analysis platforms. AcCNET allows merging phylogenetic and functional information about the concerned genomes, thus improving the capability of current methods of network analysis. The AcCNET bipartite network opens a new perspective to explore the pangenome of bacterial species, focusing on the accessory genome behind the idiosyncrasy of a particular strain and/or population.

Availability and Implementation
AcCNET is available under GNU General Public License version 3.0 (GPLv3) from http://sourceforge.net/projects/accnet

Supplementary information
Supplementary data are available at Bioinformatics online.
An accessibility-incorporated method for accurate prediction of RNA–RNA interactions from sequence data
Authors: Kato Yuki , Mori Tomoya , Sato Kengo , Maegawa Shingo , Hosokawa Hiroshi , Akutsu Tatsuya 
Published: September 2016

Abstract

Motivation
RNA–RNA interactions via base pairing play a vital role in the post-transcriptional regulation of gene expression. Efficient identification of targets for such regulatory RNAs needs not only discriminative power for positive and negative RNA–RNA interacting sequence data but also accurate prediction of interaction sites from positive data. Recently, a few studies have incorporated interaction site accessibility into their prediction methods, indicating the enhancement of predictive performance on limited positive data.

Results
Here we show the efficacy of our accessibility-based prediction model RactIPAce on newly compiled datasets. The first experiment in interaction site prediction shows that RactIPAce achieves the best predictive performance on the newly compiled dataset of experimentally verified interactions in the literature as compared with the state-of-the-art methods. In addition, the second experiment in discrimination between positive and negative interacting pairs reveals that the combination of accessibility-based methods including our approach can be effective to discern real interacting RNAs. Taking these into account, our prediction model can be effective to predict interaction sites after screening for real interacting RNAs, which will boost the functional analysis of regulatory RNAs.

Availability and Implementation
The program RactIPAce along with data used in this work is available at https://github.com/satoken/ractip/releases/tag/v1.0.1.

Supplementary information
Supplementary data are available at Bioinformatics online.
Near-infrared spectroscopy of M dwarfs. IV. A preliminary survey on the carbon isotopic ratio in M dwarfs*
Authors: Tsuji Takashi 
Published: September 2016

Abstract
Carbon isotopic ratios are estimated in 48 M dwarfs based on the medium resolution near infrared spectra (λ/Δ λ ≈ 20000) of the 13CO (3,1) band. We find clear evidence for the presence of a 13CO feature for the first time in the spectra of M dwarfs. Spectral resolution of our observed data, however, is not high enough to analyze the 13CO feature directly. Instead, we compare the observed spectrum with synthetic spectra assuming 12C/13C = 10, 25, 50, 100, and 200 for each of 48 M dwarfs and estimate the best possible 12C/13C ratio by chi-square analysis. The resulting 12C/13C ratios in M dwarfs distribute from 39 to a lower limit of 200. The mean value of 31 M dwarfs for which 12C/13C ratios are determined (i.e., excluding those with the lower limit only) is (12C/13C)dM = 87 ± 21 (p.e.), and that of 48 M dwarfs including those with the lower limit of 200 is (12C/13C)dM &gt; 127 ± 41 (p.e.). These results are somewhat larger than the 12C/13C ratio of the present interstellar matter (ISM) determined from the molecular lines observed in the millimeter and optical wavelength regions. Since the amount of 13C in the ISM has increased with time due to mass loss from evolved stars, the 12C/13C ratios in M dwarfs, reflecting those of the past ISM, should be larger than those of the present ISM. In M dwarfs, log 13C/12C plotted against log AC shows a large scatter without clear dependence on the metallicity. This result shows a marked contrast to log 16O/12C (= log AO/AC) plotted against log AC, which shows a rather tight correlation with a larger value at the lower metallicity. Such a contrast can be a natural consequence of 16O and 12C being primary products in stellar nuclear synthesis while 13C is a secondary product, at least partly.
The evolution of photospheric temperature in nova V2676 Oph toward the formation of C2 and CN during its near-maximum phase
Authors: Kawakita Hideyo , Arai Akira , Fujii Mitsugu 
Published: September 2016

Abstract
The molecular formation of C2 and CN in the dust-forming classical nova V2676 Oph occurs during its near-maximum phase. We investigated the temporal evolution of the photospheric temperature of the nova as it approached molecular formation during its early phase. The effective temperature of the nova around the maximum decreased from ∼7000 K to ∼5000 K over the course of ∼3 d. The molecules formed at temperatures of &lt;∼5000 K. Such low temperatures of nova photosphere and low expansion velocities along with carbon-rich atmosphere might play an important role in achieving conditions favorable to the molecular formation of C2 and CN in V2676 Oph.
Chronic Sarpogrelate Treatment Reveals 5-HT7 Receptor in the Serotonergic Inhibition of the Rat Vagal Bradycardia
Authors: García-Pedraza José Ángel , García Mónica , Martín María Luisa , Eleno Nélida , Morán Asunción 
Published: September 2016

Abstract:5-Hydroxytryptamine (5-HT) modulates the cardiac parasympathetic neurotransmission, inhibiting the bradyarrhythmia by 5-HT2receptor activation. We aimed to determine whether the chronic selective 5-HT2blockade (sarpogrelate) could modify the serotonergic modulation on vagal cardiac outflow in pithed rat. Bradycardic responses in rats treated with sarpogrelate (30 mg·kg−1·d−1; orally) were obtained by electrical stimulation of the vagal fibers (3, 6, and 9 Hz) or intravenous (IV) injections of acetylcholine (1, 5, and 10 μg/kg). 5-HT7receptor expression was quantified by Western blot in vagus nerve and right atrium. The IV administration of 5-HT (10–200 μg/kg) dose dependently decreased the vagally induced bradycardia, and agonists 5-CT (5-HT1/7), 8-OH-DPAT (5-HT1A), or AS-19 (5-HT7) (50 μg/kg each) mimicked the 5-HT–induced inhibitory effect. Neither agonists CGS-12066B (5-HT1B), L-694,247 (5-HT1D), nor 1-phenylbiguanide (5-HT3) modified the electrically-induced bradycardic responses. Moreover, SB-258719 (5-HT7antagonist) abolished the 5-HT–, 5-CT–, 8-OH-DPAT–, and AS-19–induced bradycardia inhibition; 5-HT or AS-19 did not modify the bradycardia induced by IV acetylcholine; and 5-HT7receptor was expressed in both the vagus nerve and the right atrium. Our outcomes suggest that blocking chronically 5-HT2receptors modifies the serotonergic influence on cardiac vagal neurotransmission exhibiting 5-HT as an exclusively inhibitory agent via prejunctional 5-HT7receptor.
Tetramethylpyrazine and Astragaloside IV Synergistically Ameliorate Left Ventricular Remodeling and Preserve Cardiac Function in a Rat Myocardial Infarction Model
Authors: Wang Chuying , Li Yumei , Yang Xinxin , Bi Shengnan , Zhang Yajie , Han Dong , Zhang Dafang 
Published: September 2016

Abstract:Tetramethylpyrazine (TMP) and astragaloside IV (AGS-IV) are herbal ingredients that have been demonstrated in animal models to limit infarct size and protect cardiomyocytes in the acute phase of myocardial infarction (MI), yet their long-term cardioprotective effects have not been evaluated. In this study, TMP and/or AGS-IV were administrated to rats for 14 days after MI. Echocardiography revealed that the left ventricular (LV) dimensions and cardiac function were preserved in the MI rats with TMP and AGS-IV treatment, compared with untreated MI rats. Moreover, the LV dimensions and cardiac function in the MI rats with TMP and AGS-IV cotreatment were comparable with the sham-operated rats. In addition, TMP and AGS-IV synergistically inhibited LV fibrosis by attenuating MI-induced collagen deposition and elevation of transforming growth factor β1. TMP and AGS-IV, alone or in synergy, enhanced angiogenesis in the infarcted myocardium and reduced cardiac hypertrophy of the remote myocardium after MI. Furthermore, TMP and AGS-IV mutually upregulated the expression of Sonic hedgehog (Shh), Smoothened, and Glioblastoma-2, the receptor and signal transducer of Shh signaling pathway, in the infarcted myocardium. In summary, in the circumstance of the irreversible ischemic injury, the antifibrotic, and pro-angiogenic properties of TMP and AGS-IV on the nonaffected tissues contribute to the cardioprotection in the healing phase post MI, and the cardioprotective effects are likely to be mediated through the Shh pathway.