bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rutin and quercetin content in the forage of common buckwheat as affected by maturity and conservation method
Authors: Mariotti Marco , Andreuccetti Victoria , Nuvoloni Roberta , Gatta Domenico , Liponi Gian Battista , Pedonese Francesca , Fratini Filippo , Turchi Barbara , Saccomanni Giuseppe , Torracca Beatrice 
Published: March 2017

AbstractThe content of rutin and quercetin was examined in fresh, hayed and ensiled forage of common buckwheat. The treatments were two varieties of buckwheat, ‘Bamby’ and ‘Lileja’, and two ripening stages of harvest, first green and first brown achenes. In the silage, produced with experimental mini‐silos, additional treatments were the wilting of the forage (at 35% dry matter) and the addition ofLactobacillus plantarumas inoculum. The concentration of rutin and quercetin decreased between ripening stages. Flavonoid content was different among varieties, ‘Lileja’ had the highest rutin concentration (about 20 g kg−1), while ‘Bamby’ had the highest quercetin concentration (about 0.2 g kg−1). Rutin and quercetin yield reached about 70 kg ha−1and 500 g ha−1, respectively. The haymaking process reduced the rutin and quercetin concentration in the forage, however, the extent of reduction was higher at the green (−43% for rutin and −55% for quercetin) than at the brown achenes stage (−13% rutin and −26% quercetin). The ensiling process, with the associated bacterial activities, led to the transformation of rutin into quercetin. The decrease of rutin in the silage, compared to fresh forage, ranged between −84 and −99%, while in contrast, the quercetin concentration increased by about 140–200 times. However, the loss of total rutin plus quercetin during ensiling was limited (approximately 5%). Forage wilting negatively affected rutin transformation in quercetin, while bacterial inoculum improved it. These results highlight that the forage of buckwheat could be considered like a dietary supplement rich of flavonoids, with the potential to be used as functional feed*.
Hidradenitis suppurativa gains increasing interest on World Wide Web: a source for patient information?
Authors: Hessam Schapoor , Salem Johannes , Bechara Falk G. , Haferkamp Axel , Heidenreich Axel , Paffenholz Pia , Sand Michael , Tsaur Igor , Borgmann Hendrik 
Published: March 2017

AbstractBackgroundGathering health information from Internet websites is increasingly utilized by patients. No data exist about hidradenitis suppurativa (HS)‐related online health information.Thus, we aimed to study the quality, popularity, readability, and timeliness of the most frequented websites on HS.MethodsGoogle Trends was used to evaluate the public interest in HS. An Internet search on Google was performed for the terms “hidradenitis suppurativa,” “acne inversa,” and “Verneuil's disease.” Readability scores, HONcode quality certification, Alexa popularity rank, and content were assessed.ResultsGoogle search queries on HS have steadily risen in the last 10 years. The website analysis revealed 39 unique websites, which were difficult to read. Ten websites (26%) had HONcode quality certification, and the median (IQR) Alexa popularity rank was 48871 (2333–361275). Thirteen websites (33%) yielded disease‐specific photos with a median rating between “quite useful” and “uncertain.” A therapy option with adalimumab was mentioned on 11 websites (28%).ConclusionsIn addition to an increasing interest, we found a broad variation in the quality, readability, popularity, and timeliness of content on HS‐related websites. Improvement of the quality and readability of HS‐related websites is desirable to potentially raise disease awareness and contribute to an earlier presentation of patients suffering with undiagnosed HS.
Systematical analyses of variants in DNase I hypersensitive sites to identify hepatocellular carcinoma susceptibility loci in a Chinese population
Authors: Jiang Tao , Du Fangzhi , Qin Na , Lu Qun , Dai Juncheng , Shen Hongbing , Hu Zhibin 
Published: March 2017

AbstractBackground and AimAlthough several variants located at coding and non‐coding regions were evaluated by previous studies, the evidence for associations between variants located in DNase I‐hypersensitive sites (DHSs) and hepatocellular carcinoma (HCC) risk was still limited. Recent advances using ENCODE data indicated that genetic variants in DHSs played an important role in carcinogenesis. Therefore, systematically investigating the associations between regulatory variants in DHSs and HCC risk should be put on the agenda.MethodsWe conducted a case–control design (1538 HCC cases vs 1465 normal controls) to evaluate the effects on HCC for the variants located at the uniform DNase I hypersensitive sites sequencing peaks in a Chinese population.ResultsWe found two novel single nucleotide polymorphisms rs12309362 (odds ratio = 0.64, P = 5.61 × 10−6) and rs9970827 (odds ratio = 0.73, P = 7.23 × 10−6) significantly associated with decreased risk of HCC. Conditional analysis proved that both of them independently contributed to the susceptibility of HCC. Expression quantitative trait loci analysis found that A allele of rs12309362 was significantly associated with an elevated expression of phosphatase phosphoglycerate mutase 5 in liver tissues. In addition, gene‐based analysis indicated that CEBPB (P = 1 × 10−4) was associated with the risk of HCC, and the expression of CEBPB was significantly lower in 50 The Cancer Genome Atlas HCC tumor tissues compared with matched normal tissues.ConclusionsOur results indicated that rs12309362 (G > A), rs9970827 (A > G) in DHSs, and elevated expression of CEBPB were associated with a decreased risk of HCC. These results may contribute us to understand the function of regulatory DNA sequences in HCC development.
RETRACTED: Effects of glucose concentration on osteogenic differentiation of type II diabetes mellitus rat bone marrow‐derived mesenchymal stromal cells on a nano‐scale modified titanium
Authors: Yamawaki I. , Taguchi Y. , Komasa S. , Tanaka A. , Umeda M. 
Published: March 2017

Background and ObjectiveDiabetes mellitus (DM) is a common disease worldwide. Patients with DM have an increased risk of losing their teeth compared with other individuals. Dental implants are a standard of care for treating partial or full edentulism, and various implant surface treatments have recently been developed to increase dental implant stability. However, some studies have reported that DM reduces osseointegration and the success rate of dental implants. The purpose of this study was to determine the effects of high glucose levels for hard tissue formation on a nano‐scale modified titanium surface.Material and MethodsTitanium disks were heated at 600°C for 1 h after treatment with or without 10 m NaOH solution. All disks were incubated with type II DM rat bone marrow‐derived mesenchymal stromal cells before exposure to one of four concentrations of glucose (5.5, 8.0, 12.0 or 24.0 mm). The effect of different glucose concentrations on bone marrow‐derived mesenchymal stromal cell osteogenesis and inflammatory cytokines on the nano‐scale modified titanium surface was evaluated.ResultsAlkaline phosphatase activity decreased with increasing glucose concentration. In contrast, osteocalcin production and calcium deposition were significantly decreased at 8.0 mm glucose, but increased with glucose concentrations over 8.0 mm. Differences in calcium/phosphate ratio associated with the various glucose concentrations were similar to osteocalcin production and calcium deposition. Inflammatory cytokines were expressed at high glucose concentrations, but the nano‐scale modified titanium surface inhibited the effect of high glucose concentrations.ConclusionHigh glucose concentration increased hard tissue formation, but the quality of the mineralized tissue decreased. Furthermore, the nano‐scale modified titanium surface increased mineralized tissue formation and anti‐inflammation, but the quality of hard tissue was dependent on glucose concentration.
Relationship between food insecurity, child weight status, and parent‐reported child eating and snacking behaviors
Authors: Kral Tanja V.E. , Chittams Jesse , Moore Reneé H. 
Published: March 2017

AbstractPurposePrior studies showed that food insecurity may increase the odds of obesity in children and adults. We still know very little about the familial aggregation of obesity in food‐insecure households or the mechanisms by which food insecurity confers an increased risk of obesity to children. The purpose of this study was to compare children and mothers from food‐insecure and food‐secure households in their weight status, child eating patterns/behaviors, and maternal feeding practices.Design and MethodsFifty mothers of 8–10‐year‐old children were asked to complete questionnaires, including the U.S. Household Food Security survey, and had their own and their children's heights and weights measured.ResultsThe odds of a child being obese were five times higher for children from food‐insecure households compared with children from food‐secure households (95% confidence interval 1.15–20.8). In univariate analyses, children from food‐insecure households showed significantly greater external eating, both past satiation and in the absence of hunger (p < .03), and mothers from food‐insecure households expressed significantly greater concern about their children's weight and used restrictive feeding practices to a greater extent (p < .03) when compared with families from food‐secure households. A greater proportion of children from food‐secure households consumed three to four snacks per day (45.9 vs. 15.4%), while a higher proportion of children from food‐insecure households consumed five or more snacks per day (15.4 vs. 0%; p = .02).Practice ImplicationsThese findings provide further support for an association between food insecurity and childhood obesity and suggest that differences in external eating, child snacking patterns, and select maternal feeding practices may be implicated in food‐insecure children's overconsumption of calories. When caring for food‐insecure children, healthcare providers should screen for problematic eating patterns and feeding practices.
Chloroprocaine for epidural anesthesia in infants and children
Authors: Veneziano Giorgio , Tobias Joseph D. 
Published: March 2017

SummaryContinuous epidural infusions are an effective and safe method of providing anesthesia and postoperative analgesia in infants and children with multiple advantages over systemic medications, including earlier tracheal extubation, decreased perioperative stress response, earlier return of bowel function, and decreased exposure to volatile anesthetic agents with uncertain long‐term neurocognitive effects. Despite these benefits, local anesthetic toxicity remains a concern in neonates and infants because of their decreased metabolic capacity for amide local anesthetics. Chloroprocaine, an ester local anesthetic agent, which is rapidly metabolized in plasma at all ages, is an attractive alternative for this special population, particularly in the presence of superimposed liver impairment or when higher infusion rates are needed for surgical incisions stretching many dermatomes. The current manuscript reviews the literature pertaining to the use of 2‐chloroprocaine for regional anesthesia in infants and children. Dosing regimens are presented and the applications of 2‐chloroprocaine in this population are discussed.
A quality improvement initiative to optimize dosing of surgical antimicrobial prophylaxis
Authors: Caruso Thomas J. , Wang Ellen , Schwenk Hayden T. , Scheinker David , Yeverino Calida , Tweedy Mary , Maheru Manjit , Sharek Paul J. 
Published: March 2017

SummaryBackgroundThe risk of surgical site infections is reduced with appropriate timing and dosing of preoperative antimicrobials. Based on evolving national guidelines, we increased the preoperative dose of cefazolin from 25 to 30 mg·kg−1. This quality improvement project describes an improvement initiative to develop standard work processes to ensure appropriate dosing.AimsThe primary aim was to deliver cefazolin 30 mg·kg−1 to at least 90% of indicated patients. The secondary aim was to determine differences between accuracy of cefazolin doses when given as an electronic order compared to a verbal order.MethodsData were collected from January 1, 2012 to May 31, 2016. A quality improvement team of perioperative physicians, nurses, and pharmacists implemented a series of interventions including new electronic medical record order sets, personal provider antibiotic dose badges, and utilization of pharmacists to prepare antibiotics to increase compliance with the recommended dose. Process compliance was measured using a statistical process control chart, and dose compliance was measured through electronic analysis of the electronic medical record. Secondary aim data were displayed as percentage of dose compliance. An unpaired t‐test was used to determine differences between groups.ResultsBetween January 1, 2012 and May 31, 2016, cefazolin was administered to 9086 patients. The mean compliance of cefazolin at 30 mg·kg−1 from May 2013 to March 2014 was 40%, which prompted initiation of this project. From April 2014 to May 2016, a series of interventions were deployed. The mean compliance from September 2015 to May 2016 was 93% with significantly reduced variation and no special cause variation, indicating that the process was in control at the target primary aim.There were 649 cefazolin administrations given verbally and 1929 given with an electronic order between October 1, 2014 and May 31, 2016. During this time period, the rate of compliance of administering cefazolin at 30 mg·kg−1 was significantly higher when given after an electronic order than when given verbally, 94% vs 76%.ConclusionThis comprehensive quality improvement project improved practitioner compliance with evidence‐based preoperative antimicrobial dosing recommendations to reduce the risk of surgical site infections.
Risk factors for perioperative mortality and transfusion in sacrococcygeal teratoma resections
Authors: Isserman Rebecca S. , Nelson Olivia , Tran Kha M. , Cai Lingyu , Polansky Marcia , Rosenbloom Julia M. , Goebel Theodora K. , Lin Elaina E. 
Published: March 2017

SummaryBackgroundSacrococcygeal teratomas are a common congenital tumor. Surgical resection can occur in utero, in the neonatal period, or in the postneonatal period.AimsWe describe patient and tumor factors associated with mortality and transfusion in this population.MethodsWe did a retrospective chart review of patients who underwent sacrococcygeal teratoma resection between January 1998 and March 2016. Demographic data, transfusion data, and tumor characteristics were collected. Descriptive statistics were calculated, and univariate comparisons were performed with chi‐square test and Fisher's exact test. Variables significant at univariate level were used in multivariate logistic regression and negative binomial regression.ResultsOf the 112 cases, 6 were in utero repairs, 73 were neonatal repairs, and 33 were repairs at >30 days of life. There was 17%, 1%, and 0% intraoperative mortality and 33%, 5%, and 0% 30‐day mortality in the in utero, neonatal, and >30 days of life repairs, respectively. All six patients who died within the first 30 days of life had a postmenstrual age of <32 weeks at time of surgery. All six patients who died had noncystic tumors. Patients with noncystic tumors were more likely to be born prior to 30‐week gestation (23/65 vs 6/47; χ2 = 7.3; P = 0.007). Gestational age >30 weeks was associated with decreased intraoperative death (0% vs 10%; modified maximum likelihood estimate of OR 0.05; 95% CI 0.002–0.96; P = 0.02). Gestational age >30 weeks (2.4% vs 13.8%; OR 0.15; 95% CI 0.03–0.89; P = 0.04) and cystic morphology (0% vs 9.2%; modified maximum likelihood estimate of OR 0.1; CI 0.01–1.75; P = 0.04) were associated with decreased 30‐day mortality and emergent surgery (17.9% vs 1.2%; OR 18; 95% CI 2–162.2; P = 0.004) was associated with increased 30‐day mortality. Gestational age >30 weeks (33.7% vs 62.1%; OR 0.27; 95% CI 0.09–0.79; P = 0.02) and Altman class 3–4 (12.1% vs 52.7%; OR 0.1; 95% CI 0.03–0.34; P = 0.0002) were associated with decreased need for transfusion and noncystic tumor was associated with increased transfusion volume (131.6 ml·kg−1 [95% CI 94–184] vs 63 ml·kg−1 [95% CI 40–100.1]; P = 0.01).ConclusionsPrematurity is associated with increased intraoperative and 30‐day mortality. Noncystic tumor morphology was the only significant factor associated with transfusion volume and all six patients who died had transfusion volumes of 240 ml·kg−1 or greater. In these patients at high risk of mortality due to blood loss, the anesthesia team should be prepared to manage massive transfusion and coagulopathy with blood components and pharmacologic measures.
Disruptions in valine degradation affect seed development and germination in Arabidopsis
Authors: Gipson Andrew B. , Morton Kyla J. , Rhee Rachel J. , Simo Szabolcs , Clayton Jack A. , Perrett Morgan E. , Binkley Christiana G. , Jensen Erika L. , Oakes Dana L. , Rouhier Matthew F. , Rouhier Kerry A. 
Published: March 2017

SummaryWe have functionally characterized the role of two putative mitochondrial enzymes in valine degradation using insertional mutants. Prior to this study, the relationship between branched‐chain amino acid degradation (named for leucine, valine and isoleucine) and seed development was limited to leucine catabolism. Using a reverse genetics approach, we show that disruptions in the mitochondrial valine degradation pathway affect seed development and germination in Arabidopsis thaliana. A null mutant of 3‐hydroxyisobutyryl‐CoA hydrolase (CHY4, At4g31810) resulted in an embryo lethal phenotype, while a null mutant of methylmalonate semialdehyde dehydrogenase (MMSD, At2g14170) resulted in seeds with wrinkled coats, decreased storage reserves, elevated valine and leucine, and reduced germination rates. These data highlight the unique contributions CHY4 and MMSD make to the overall growth and viability of plants. It also increases our knowledge of the role branched‐chain amino acid catabolism plays in seed development and amino acid homeostasis.
Group O RBCs: where is universal donor blood being used
Authors: Barty R. L. , Pai M. , Liu Y. , Arnold D. M. , Cook R. J. , Zeller M. P. , Heddle N. M. 
Published: March 2017

BackgroundThere have been recurrent shortages of group O blood due to insufficient inventory and use of group O blood in ABO non‐identical recipients. We performed a 12‐year retrospective study to determine utilization of group O Rh‐positive and Rh‐negative red blood cells (RBCs) by recipient ABO group. Reasons for transfusing group O blood to ABO non‐identical recipients were also assessed.MethodsUtilization data from all group O Rh‐positive and Rh‐negative RBCs transfused at three academic hospitals between April 2002 and March 2014 were included. Data were extracted from Transfusion Registry for Utilization Surveillance and Tracking, a comprehensive database with inventory information on all blood products received at the hospitals. Extracted data included product type, ABO and Rh, final disposition (transfused, wasted, outdated), and demographic and clinical data on all patients admitted to hospital. Descriptive statistics were performed using sas 9.3.ResultsThere were 314 968 RBC transfusions: 151 645 (48·1%) were group O, of which 138 136 (91·1%) RBC units were transfused to group O individuals. ABO non‐identical recipients received 13 509 group O RBCs (8·9%). The percentage of group O RBCs transfused to ABO non‐identical recipients by fiscal year varied from 7·8% to 11·1% with a steady increase from 2011 to 2013. Reasons for this included: trauma, outdating, outpatient usage and shortages.ConclusionThe practice of transfusing O RBCs to non‐O individuals has been increasing. Specific hospital and blood supplier policies could be targeted to change practice, leading to a more sustainable group O red blood cell supply.
In VitroandIn VivoAntibacterial Activities of Patchouli Alcohol, a Naturally Occurring Tricyclic Sesquiterpene, against Helicobacter pylori Infection
Authors: Xu Y. F. , Lian D. W. , Chen Y. Q. , Cai Y. F. , Zheng Y. F. , Fan P. L. , Ren W. K. , Fu L. J. , Li Y. C. , Xie J. H. , Cao H. Y. , Tan B. , Su Z. R. , Huang P. 
Published: March 2017

ABSTRACTThis study further evaluated thein vitroandin vivoanti-Helicobacter pyloriactivities and potential underlying mechanism of patchouli alcohol (PA), a tricyclic sesquiterpene. In thein vitroassay, the capacities of PA to inhibit and killH. pyloriwere tested on three standard strains at different pH values and on 12 clinical isolates. The effects of PA onH. pyloriadhesion (and itsalpA,alpB, andbabAgenes), motility (and itsflaAandflaBgenes), ultrastructure, and flagellation were investigated. Moreover, theH. pyloriresistance to and postantibiotic effect (PAE) of PA were determined. Furthermore, thein vivoeffects of PA onH. pylorieradication and gastritis were examined. Results showed that MICs of PA against three standard strains (pH 5.3 to 9) and 12 clinical isolates were 25 to 75 and 12.5 to 50 μg/ml, respectively. The killing kinetics of PA were time and concentration dependent, and its minimal bactericidal concentrations (MBCs) were 25 to 75 μg/ml. In addition,H. pyloriadhesion, motility, ultrastructure, and flagellation were significantly suppressed. PA also remarkably inhibited the expression of adhesion genes (alpAandalpB) and motility genes (flaAandflaB). Furthermore, PA treatment caused a longer PAE and less bacterial resistance than clarithromycin and metronidazole. Thein vivostudy showed that PA can effectively eradicateH. pylori, inhibit gastritis, and suppress the expression of inflammatory mediators (COX-2, interleukin 1β, tumor necrosis factor alpha, and inducible nitric oxide synthase [iNOS]). In conclusion, PA can efficiently killH. pylori, interfere with its infection process, and attenuate gastritis with less bacterial resistance, making it a potential candidate for new drug development.