bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cohesin can remain associated with chromosomes during DNA replication
Authors: Rhodes James D. P. , Haarhuis Judith H. I. , Grimm Jonathan B. , Rowland Benjamin D. , Lavis Luke D. , Nasmyth Kim A. 
Published: April 2017

SummaryTo ensure disjunction to opposite poles during anaphase, sister chromatids must be held together following DNA replication. This is mediated by cohesin, which is thought to entrap sister DNAs inside a tripartite ring composed of its Smc and kleisin (Scc1) subunits. How such structures are created during S phase is poorly understood, in particular whether they are derived from complexes that had entrapped DNAs prior to replication. To address this, we used selective photobleaching to determine whether cohesin associated with chromatin in G1 persists in situ after replication. We used unlabelled HaloTag ligand following fluorescent labelling to block newly synthesised Halo-tagged Scc1 protein from incorporating fluorescent dye (pulse-chase or pcFRAP). In cells whose cohesin turnover is inactivated by deletion ofWAPL, Scc1 remains associated with chromatin throughout S phase. These findings suggest that cohesion might be generated by cohesin that is already bound to unreplicated DNA.
SEARCH_16S: A new algorithm for identifying 16S ribosomal RNA genes in contigs and chromosomes
Authors: Edgar Robert C. 
Published: April 2017

AbstractSEARCH_16S is a new algorithm that annotates 16S ribosomal RNA genes in microbial genomes and metagenomic sequences. Word counting is used to identify candidate segments, then conserved motifs are used to identify homologous loci close to the gene boundaries. SEARCH_16S has >99% sensitivity to known 16S genes.
Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering
Authors: Higgins Sean A. , Ouonkap Sorel , Savage David F. 
Published: April 2017

ABSTRACTComprehensive and programmable protein mutagenesis is critical for understanding structure-function relationships and improving protein function. However, current techniques enabling comprehensive protein mutagenesis are based on PCR and requirein vitroreactions involving specialized protocols and reagents. This has complicated efforts to rapidly and reliably produce desired comprehensive protein libraries. Here we demonstrate that plasmid recombineering is a simple and robustin vivomethod for the generation of protein mutants for both comprehensive library generation as well as programmable targeting of sequence space. Using the fluorescent protein iLOV as a model target, we build a complete mutagenesis library and find it to be specific and unbiased, detecting 99.8% of our intended mutations. We then develop a thermostability screen and utilize our comprehensive mutation data to rapidly construct a targeted and multiplexed library that identifies significantly improved variants, thus demonstrating rapid protein engineering in a simple one-pot protocol.
Advertising effectiveness on financial performance of banking sector: Turkey case
Authors: Acar Merve , Temiz Hüseyin 
Published: April 2017

PurposeThe purpose of this paper is to analyze the association between banks’ advertising expenses and accounting measures of income and profitability for banking sector.Design/methodology/approachIn this study the authors have used distributed lag models to investigate the association between advertising expenses and banks’ financial performance. To investigate the long-term effect of advertising expenses on financial performance of banking sector Koyck’s distributed lag models have been used.FindingsThe results confirm a significant and positive association between advertising expenses and financial performance. Besides its positive effect, the authors provide a basis for detecting the extent to which advertising has long-term benefits. The results show a positive association between advertising expenses and financial performance that extend that extends over time, thereby suggesting that advertising expenses should be capitalized and then amortized instead of being incurred as an expense immediately.Originality/valueAlthough there are lots of studies about advertising and its effect on financial position of firms, research about advertising effects on financial performance of banking sector is very scarce. Therefore, this study has a potential to shed light on research about marketing aspect of financial sector. Besides, empirical results show a positive association between advertising expenses and financial performance that extend that extends over time (interest income, total operating income and return on assets), thereby suggesting that advertising expenses should be capitalized and then amortized instead of being incurred as an expense immediately. To sum it all, the paper finds an evidence for banking sector that advertising inholds “future economic benefits” which is the key criterion necessary for asset recognition.
The mediating role of hedonic motivation on the relationship between adoption of e-banking and its determinants
Authors: Salimon Maruf Gbadebo , Yusoff Rushami Zien Bin , Mohd Mokhtar Sany Sanuri 
Published: April 2017

Purpose
The purpose of this paper is to examine the combined roles that perceived usefulness (PU), perceived ease of use, perceived security and hedonic motivation play on the adoption of e-banking. It also aims to determine the ability of hedonic motivation to transfer the effect of other determinants of e-banking adoption.

Design/methodology/approach
In order to empirically test the conceptual model of this study, data were collected from the users of e-banking in Nigeria. A total of 266 questionnaires were analyzed using partial least square structural equation modeling.

Findings
The empirical results revealed a significant and positive relationship between PU, perceived security and e-banking adoption. The same result was found to be applicable to the relationship between PU, perceived ease of use, perceived security and hedonic motivation. However, support was not found for the relationship between perceived ease of use and adoption of e-banking. Hedonic motivation plays a mediating role between PU, perceived security and e-banking adoption. The study did not find a mediating effect of hedonic motivation between perceived ease of use and e-banking adoption.

Practical implications
The results of this study provide insights for banking practitioners to know which aspect of e-banking to improve and to aid with policies that will increase adoption. Furthermore, improvements in hedonic motivation will also significantly increase adoption of e-banking.

Originality/value
This study is one of the pioneer studies that tests the mediating influence of hedonic motivation.
Impact of brand familiarity on brands experience dimensions for financial services brands
Authors: Bapat Dhananjay 
Published: April 2017

Purpose
The purpose of this paper is to explore the impact of brand familiarity on the various dimensions of brand experience, and to identify the factor structure of brand familiarity for financial services brands.

Design/methodology/approach
This study used a convenience sampling technique by contacting 216 respondents, and examined the relationship between brand experience dimensions and brand familiarity. An independent sample t-test was performed to assess the differences for brand experience dimensions. Exploratory and confirmatory factor analyses were performed for both low familiarity and high familiarity service brands to highlight the differences.

Findings
The improvement in brand familiarity is positive for sensory, emotional, behavioral and relational brand experiences for high familiarity service brands. Exploratory factor analysis and confirmatory factor analysis found a four-factor brand experience model for low brand familiarity and a five-factor brand experience structure for high familiarity financial services brands. The study of financial services brands validates the service brand experience framework of Nysveen et al. (2013) for high familiarity brands, but not for low familiarity financial services brand.

Practical implications
There is a need for marketers to comprehend various dimensions of brand experience in the context of financial services brands which are experiencing increased competition with non-banks.

Originality/value
The study makes a contribution to the existing literature as the concept of brand familiarity and its relationship with brand experience have received scant attention in the past.
Principles of successful implementation of lecture recordings in higher education
Authors: Ollermann Frank , Rolf Rüdiger , Greweling Christian , Klaßen André 
Published: April 2017

Purpose
This paper aims to describe the principles underlying the successful implementation of a lecture recording service in higher education.

Design/methodology/approach
The paper qualitatively reviews the practices and experiences of several years of automated lecture recording at a medium-sized university in Germany.

Findings
The paper concludes that there are several principles that should be followed to successfully implement lecture recordings in higher education.

Practical implications
The principles described in this paper can serve as recommendations for other universities that would like to establish or optimize their own lecture recording service.

Originality/value
The value of the paper lies mainly in the great amount of experience in successfully running a lecture recording service on which the principles and recommendations are based.
The impact of socio-political and economic environments on private sector participation in energy infrastructure delivery in Ghana
Authors: Owusu-Manu De-Graft , Edwards David John , Kutin-Mensah E.K. , Kilby Angela , Parn Erika , Love Peter Edward 
Published: April 2017

Purpose
Investment in power and electricity generation for replacing aging infrastructure with new represents a major challenge for developing countries. This paper therefore aims to examine infrastructure projects’ characteristics and how socio-political and economic investment environments interplay to influence the degree of private sector participation (PPP) in infrastructure delivery in Ghana.

Design/methodology/approach
Using World Bank Public-private infrastructure advisory facility (PPIAF) and private participation in infrastructure (PPI) project database data from 1994 to 2013, binary logistic regression was used to: determine the probability of a higher or lower degree of PPP; and examine the significance of factors that are determinants of private investments.

Findings
The findings reveal that the private sector is more likely to invest in a higher degree of PPP infrastructure projects through greenfield and concession vehicles as opposed to management and leasing contracts. From the extant literature, drivers of PPP included infrastructure project characteristics and the social–economic–political health of the host country. However, the significance, direction and magnitude of these drivers vary.

Originality/value
This paper identifies investment drivers to PPP advisors and project managers and seeks to engender discussion among government policymakers responsible for promoting and managing PPP projects. Direction for future work seeks to explore competitive routes to infrastructure debt and equity finance options that finance energy projects.
Intuitive design: framing a software test system as a status reporting tool for business
Authors: So Chaehan 
Published: April 2017

Purpose
This paper aims to present a conceptual framework of how software teams can leverage the implicit information of implemented acceptance tests to cater to the needs of decision makers. The research questions on this framework were how business stakeholders can receive project status information in an intuitive way and how this framework can guarantee the traceability of tests to requirements.

Design/methodology/approach
The conceptual framework delineates the design of an acceptance test framework in three aspects: how the requirements model reflects the evolving states of requirement maturity over a project, how the acceptance test model becomes synchronized with the requirements model without a traceability matrix and how the acceptance test model communicates business value to the decision makers.

Findings
In an industrial case study, the presented framework yielded the positive effects of intuitive understanding by business stakeholders, high test coverage of requirements and distinctly reduced manual quality assurance (QA) work by automated testing for browsers and mobile devices.

Practical implications
The presented framework can help to convince business stakeholders to approve the budget for building a testing framework because it delivers them value as a status reporting tool.

Originality/value
This paper is the first to describe a step-by-step approach to solving a critical problem that IT departments frequently face. The solution consists in a new way of transforming the perception of a technical framework into a reporting tool for business information by intuitive design. The idea of mapping hierarchically corresponding abstraction layers can be transferred to other engineering domains.
Getting acquainted with social networks and apps: periscope up! Broadcasting via live streaming
Authors: Anderson Katie Elson 
Published: April 2017

Purpose
The purpose of this paper is to get acquainted with social networks and apps: up periscope! broadcasting via live streaming.

Design/methodology/approach
This study is a general review paper.

Findings
The popularity and growth of live streaming applications should not be overlooked by information professionals.

Originality/value
Even if the library does not use the app itself, it is important to be aware that patrons are using these tools to stream their lives to the world.
Use of a duckweed pond for the domestic wastewater polishing in Ilha Solteira, SP, Brazil
Authors: Garcia Daiane Cristina de Oliveira , Albertin Liliane Lazzari , Matsumoto Tsunao 
Published: April 2017

PurposeThe purpose of this paper is to evaluate the efficiency of a duckweed pond in the polishing of a stabilization pond effluent, as well as quantify its biomass production. Once an adequate destination is given to the produced biomass, the wastewater treatment plant can work in a sustainable and integrated way.Design/methodology/approachThe duckweed pond consisted of a tank with volume 0.44 m3, operating in continuous flow with an outflow of 0.12 m3/day and hydraulic retention time of 3.8 days. Effluent samples were collected before and after the treatment, with analyzes made: daily-pH, dissolved oxygen and temperature; twice a week – total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (COD); and weekly – total solids (TS) and Biochemical Oxygen Demand (BOD5). The duckweeds were collected each for seven days for its production quantification.FindingsThe highest efficiency of TN, TP, COD, BOD5and TS removal were of 74.67, 66.18, 88.12, 91.14 and 48.9 percent, respectively. The highest biomass production rate was 10.33 g/m2/day in dry mass.Research limitations/implicationsThere was great variation in biomass production, which may be related to the stabilization pond effluent conditions. The evaluation of the effluent composition, which will be treated with duckweeds, is recommended.Practical implicationsThe evaluated treatment system obtained positive results for the reduction in the analyzed variables concentration, being an efficient technology and with operational simplicity for the domestic effluent polishing.Originality/valueThe motivation of this work was to bring a simple system of treatment and to give value to a domestic wastewater treatment system in a way that, at the same time the effluent polluter level is reduced and it is also possible to produce biomass during the treatment process.
Particle size characterization as a support for sediment contamination analysis
Authors: Fernandes Felippe , Poleto Cristiano 
Published: April 2017

Purpose
The purpose of this paper is to analyse the results of particle size profiles carried out in the dam Mãe d’água, located in the metropolitan region of Porto Alegre, Rio Grande do Sul, becoming a tool of support for geochemistry evaluation works of sediments in this basin.

Design/methodology/approach
The methodology comprised the particle size analysis of eight cores sampled using the extraction profile technique “Sampling Cores.” A total of 56 sub-samples were obtained at different depths, varying between 0 and 52 cm. The particle size analysis of the sub-samples was carried out with the protocol relating to Brazilian analysis standards NBR 7,181; for determination of the particle size distribution of thick sediments, the sieve method was used, and for fine sediments, the pipette method was used.

Findings
The results show that for the different depths, the sediment profiles show similarity in their sedimentary deposition layers. Cores T1, T6, T7 and T8 showed a classification with predominance of mud. Cores T2 and T5 showed variations throughout their sedimentary profile, varying of mud predominance to sand with gravel, and on cores T3 and T4, the predominant characteristic is sand with mud and sand with gravel, respectively.

Originality/value
It can be seen in this paper that energy of sedimentological flow reaches it in a different way, creating these three sectors (first, sand with gravel; second, sand with mud and third, mud) with different characteristics – morphodynamic and sedimentological. These characteristics are important for the study of the heavy metals on the soil and therefore for reducing the risk of contamination of water courses.
From pristine forests to high‐altitude pastures: an ecological approach to prehistoric human impact on vegetation and landscapes in the western Italian Alps
Authors: Pini Roberta , Ravazzi Cesare , Raiteri Luca , Guerreschi Antonio , Castellano Lorenzo , Comolli Roberto 
Published: April 2017

Summary

This paper addresses the origin and development of the oldest prehistoric pasture in the timberline ecotone known so far in the Alps and its relation to anthropogenic pressure and natural climate change.

Palaeoecological and geochemical techniques were applied on the Crotte Basse mire stratigraphy (2365 m a.s.l, northwestern Italy) to describe changes in vegetation composition, forest biomass, land use and fertilization between c. 6400–1800 cal years bp.

Subalpine forests dominated by Pinus cembra occurred at very high‐altitude up to c. 5600 cal years bp, when a sharp contraction of woody vegetation took place. This major vegetation shift is matched by increasing charcoal input and markers of pastoral/grazing activities (pollen, dung spores and forms of phosphorus) in the sediment sequence in this small basin.

Major phases of landscape change detected in our multiproxy record chronologically match intervals of cumulative probability density of 14C ages from nearby archaeological sites, suggesting that human activity was the factor leading to massive landscape change from the onset of the Copper Age (c. 5600 cal years bp). The change may have been reinforced by climate variability in the period 5700–5300 cal years bp.

Sensitivity of woody species to fires was statistically explored (Appendix S1, Supporting Information), revealing negative reactions of P. cembra and Betula to frequent fire episodes and positive reactions of Alnus viridis and Juniperus. Fire episodes do not affect Larix dynamics.

Synthesis. Mt. Fallère provides some of the oldest and consistent evidence so far available in the Alps for major anthropogenic pressure at the upper forest limit. As far back as 5600 cal years bp, high‐elevation forest ecosystems were permanently disrupted and the alpine pastures were created. Palaeoecological data enable a clear distinction between a random and sporadic use of the alpine space, typical for Mesolithic and Neolithic societies, and an organized seasonal exploitation of natural resources, starting from the Copper Age onwards. The chronological comparison of independent climate proxies, palaeoecological information and pollen‐based temperature reconstructions sheds light on the relationships between climate and humans since prehistoric times.
Simulation in Occupational Therapy Curricula: A literature review
Authors: Bennett Sally , Rodger Sylvia , Fitzgerald Cate , Gibson Libby 
Published: April 2017

Background/aimSimulated learning experiences are increasingly being used in health‐care education to enhance student engagement and provide experiences that reflect clinical practice; however, simulation has not been widely investigated in occupational therapy curricula. The aim of this paper was to: (i) describe the existing research about the use and evaluation of simulation over the last three decades in occupational therapy curricula and (ii) consider how simulation has been used to develop competence in students.MethodsA literature review was undertaken with searches of MEDLINE, CINAHL and ERIC to locate articles that described or evaluated the use of simulation in occupational therapy curricula.Results/findingsFifty‐seven papers were identified. Occupational therapy educators have used the full scope of simulation modalities, including written case studies (22), standardised patients (13), video case studies (15), computer‐based and virtual reality cases (7), role‐play (8) and mannequins and part‐task trainers (4). Ten studies used combinations of these modalities and two papers compared modalities. Most papers described the use of simulation for foundational courses, as for preparation for fieldwork, and to address competencies necessary for newly graduating therapists. The majority of studies were descriptive, used pre‐post design, or were student's perceptions of the value of simulation.ConclusionSimulation‐based education has been used for a wide range of purposes in occupational therapy curricula and appears to be well received. Randomised controlled trials are needed to more accurately understand the effects of simulation not just for occupational therapy students but for longer term outcomes in clinical practice.
Taxonomic variability and functional stability in microbial communities infected by phages
Authors: Louca Stilianos , Doebeli Michael 
Published: April 2017

SummaryMicrobial communities can display large variation in taxonomic composition, yet this variation can coincide with stable metabolic functional structure and performance. The mechanisms driving the taxonomic variation within functional groups remain largely unknown. Biotic interactions, such as predation by phages, have been suggested as potential cause of taxonomic turnover, but the conditions for this scenario have not been rigorously examined. Further, it is unknown how predation by phages affects community function, and how these effects are modulated by functional redundancy in the communities. Here, we address these questions using a model for a methanogenic microbial community that includes several interacting metabolic functional groups. Each functional group comprises multiple competing clades, and each clade is attacked by a specialist lytic phage. Our model predicts that phages induce intense taxonomic turnover, resembling the variability observed in previous experiments. The functional structure and performance of the community are also disturbed by phage predation, but they become more stable as the functional redundancy in the community increases. The extent of this stabilization depends on the particular functions considered. Our model suggests mechanisms by which functional redundancy stabilizes community function and supports the interpretation that biotic interactions promote taxonomic turnover within microbial functional groups.