bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaleidoscope
Authors: Tracy Derek K. , Joyce Dan W. , Shergill Sukhwinder S. 
Published: January 2017

Tapping into your New Year resolutions, we present some cautionary data to consider.Recent research on novel addiction types suggests that, in addition to hunger, the urge to eat is considerably influenced by environmental cues. Obese individuals have been shown to be more sensitive to these, with subsequent stronger craving and larger portion sizes. Neurobiologically, this has been linked with pathological alterations to cue-triggered motivational responses in the nucleus accumbens (NAc) parallel to those seen in drug addiction. Oginsky et al investigated the effects of a junk-food diet on obesity-susceptible and obesity-resistant rats. Glutamatergic calcium-permeable AMPA receptor (CP-AMPAR) functioning in the NAc showed more rapid and long-lasting (for weeks after cessation of junk-food consumption) increases in the obesity-sensitive group. CP-AMPARs mediate cue-triggered food seeking, and their changes in the brain occurred before weight gain. The data support the concept that ‘junk food addiction’ induced by the consumption of fatty and high-sugar foods may be contributing to the obesity epidemic.
An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates
Authors: Gays Dafne , Hess Christopher , Camporeale Annalisa , Ala Ugo , Provero Paolo , Mosimann Christian , Santoro Massimo M. 
Published: January 2017

ABSTRACT
Intestinal smooth muscle cells (iSMCs) are a crucial component of the adult gastrointestinal tract and support intestinal differentiation, peristalsis and epithelial homeostasis during development. Despite these crucial roles, the origin of iSMCs and the mechanisms responsible for their differentiation and function remain largely unknown in vertebrates. Here, we demonstrate that iSMCs arise from the lateral plate mesoderm (LPM) in a stepwise process. Combining pharmacological and genetic approaches, we show that TGFβ/Alk5 signaling drives the LPM ventral migration and commitment to an iSMC fate. The Alk5-dependent induction of zeb1a and foxo1a is required for this morphogenetic process: zeb1a is responsible for driving LPM migration around the gut, whereas foxo1a regulates LPM predisposition to iSMC differentiation. We further show that TGFβ, zeb1a and foxo1a are tightly linked together by miR-145. In iSMC-committed cells, TGFβ induces the expression of miR-145, which in turn is able to downregulate zeb1a and foxo1a. The absence of miR-145 results in only a slight reduction in the number of iSMCs, which still express mesenchymal genes but fail to contract. Together, our data uncover a cascade of molecular events that govern distinct morphogenetic steps during the emergence and differentiation of vertebrate iSMCs.
The Ectocarpus IMMEDIATE UPRIGHT gene encodes a member of a novel family of cysteine-rich proteins with an unusual distribution across the eukaryotes
Authors: Macaisne Nicolas , Liu Fuli , Scornet Delphine , Peters Akira F. , Lipinska Agnieszka , Perrineau Marie-Mathilde , Henry Antoine , Strittmatter Martina , Coelho Susana M. , Cock J. Mark 
Published: January 2017

ABSTRACT
The sporophyte generation of the brown alga Ectocarpus sp. exhibits an unusual pattern of development compared with the majority of brown algae. The first cell division is symmetrical and the apical-basal axis is established late in development. In the immediate upright (imm) mutant, the initial cell undergoes an asymmetric division to immediately establish the apical-basal axis. We provide evidence which suggests that this phenotype corresponds to the ancestral state of the sporophyte. The IMM gene encodes a protein of unknown function that contains a repeated motif also found in the EsV-1-7 gene of the Ectocarpus virus EsV-1. Brown algae possess large families of EsV-1-7 domain genes but these genes are rare in other stramenopiles, suggesting that the expansion of this family might have been linked with the emergence of multicellular complexity. EsV-1-7 domain genes have a patchy distribution across eukaryotic supergroups and occur in several viral genomes, suggesting possible horizontal transfer during eukaryote evolution.
Stage- and subunit-specific functions of polycomb repressive complex 2 in bladder urothelial formation and regeneration
Authors: Guo Chunming , Balsara Zarine R. , Hill Warren G. , Li Xue 
Published: January 2017

ABSTRACT
Urothelium is the protective lining of the urinary tract. The mechanisms underlying urothelial formation and maintenance are largely unknown. Here, we report the stage-specific roles of PRC2 epigenetic regulators in embryonic and adult urothelial progenitors. Without Eed, the obligatory subunit of PRC2, embryonic urothelial progenitors demonstrate reduced proliferation with concomitant dysregulation of genes including Cdkn2a (p16), Cdkn2b (p15) and Shh. These mutants display premature differentiation of keratin 5-positive (Krt5+) basal cells and ectopic expression of squamous-like differentiation markers. Deletion of Ezh2, the major enzymatic component of PRC2, causes upregulation of Upk3a+ superficial cells. Unexpectedly, Eed and Eed/Ezh2 double mutants exhibit delayed superficial cell differentiation. Furthermore, Eed regulates the proliferative and regenerative capacity of adult urothelial progenitors and prevents precocious differentiation. Collectively, these findings uncover the epigenetic mechanism by which PRC2 controls urothelial progenitor cell fate and the timing of differentiation, and further suggest an epigenetic basis of urothelial maintenance and regeneration.
The why, when and how of lipid droplet diversity
Authors: Thiam Abdou Rachid , Beller Mathias 
Published: January 2017

ABSTRACT
Lipid droplets are the universal cellular organelles for the transient or long-term storage of lipids. The number, size and composition of lipid droplets vary greatly within cells in a homogenous population as well as in different cell types. The variability of intracellular lipid-storage organelles reflects the diversification of lipid droplet composition and function. Lipid droplet diversification results, for example, in two cellular lipid droplet populations that are prone to diminish and grow, respectively. The aberrant accumulation or depletion of lipids are hallmarks or causes of various human pathologies. Thus, a better understanding of the origins of lipid droplet diversification is not only a fascinating cell biology question but also potentially serves to improve comprehension of pathologies that entail the accumulation of lipids. This Commentary covers the lipid droplet life cycle and highlights the early steps during lipid droplet biogenesis, which we propose to be the potential driving forces of lipid droplet diversification.
Sideroflexin 3 is an α-synuclein-dependent mitochondrial protein that regulates synaptic morphology
Authors: Amorim Inês S. , Graham Laura C. , Carter Roderick N. , Morton Nicholas M. , Hammachi Fella , Kunath Tilo , Pennetta Giuseppa , Carpanini Sarah M. , Manson Jean C. , Lamont Douglas J. , Wishart Thomas M. , Gillingwater Thomas H. 
Published: January 2017

ABSTRACT
α-Synuclein plays a central role in Parkinson's disease, where it contributes to the vulnerability of synapses to degeneration. However, the downstream mechanisms through which α-synuclein controls synaptic stability and degeneration are not fully understood. Here, comparative proteomics on synapses isolated from α-synuclein−/− mouse brain identified mitochondrial proteins as primary targets of α-synuclein, revealing 37 mitochondrial proteins not previously linked to α-synuclein or neurodegeneration pathways. Of these, sideroflexin 3 (SFXN3) was found to be a mitochondrial protein localized to the inner mitochondrial membrane. Loss of SFXN3 did not disturb mitochondrial electron transport chain function in mouse synapses, suggesting that its function in mitochondria is likely to be independent of canonical bioenergetic pathways. In contrast, experimental manipulation of SFXN3 levels disrupted synaptic morphology at the Drosophila neuromuscular junction. These results provide novel insights into α-synuclein-dependent pathways, highlighting an important influence on mitochondrial proteins at the synapse, including SFXN3. We also identify SFXN3 as a new mitochondrial protein capable of regulating synaptic morphology in vivo.
The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants
Authors: Poulet Axel , Duc Céline , Voisin Maxime , Desset Sophie , Tutois Sylvie , Vanrobays Emmanuel , Benoit Matthias , Evans David E. , Probst Aline V. , Tatout Christophe 
Published: January 2017

ABSTRACT
The linker of nucleoskeleton and cytoskeleton (LINC) complex is an evolutionarily well-conserved protein bridge connecting the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data support its function in nuclear morphology and meiosis, its involvement in chromatin organisation has not been studied in plants. Here, 3D imaging methods have been used to investigate nuclear morphology and chromatin organisation in interphase nuclei of the model plant Arabidopsis thaliana in which heterochromatin clusters in conspicuous chromatin domains called chromocentres. Chromocentres form a repressive chromatin environment contributing to transcriptional silencing of repeated sequences, a general mechanism needed for genome stability. Quantitative measurements of the 3D position of chromocentres indicate their close proximity to the nuclear periphery but that their position varies with nuclear volume and can be altered in specific mutants affecting the LINC complex. Finally, we propose that the plant LINC complex contributes to proper heterochromatin organisation and positioning at the nuclear periphery, since its alteration is associated with the release of transcriptional silencing as well as decompaction of heterochromatic sequences.
ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2–MRCKα balance
Authors: Bouin Anne-Pascale , Kyurmurkov Alexander , Régent-Kloeckner Myriam , Ribba Anne-Sophie , Faurobert Eva , Fournier Henri-Noël , Bourrin-Reynard Ingrid , Manet-Dupé Sandra , Oddou Christiane , Balland Martial , Planus Emmanuelle , Albiges-Rizo Corinne 
Published: January 2017

ABSTRACT
Cell migration is a complex process requiring density and rigidity sensing of the microenvironment to adapt cell migratory speed through focal adhesion and actin cytoskeleton regulation. ICAP-1 (also known as ITGB1BP1), a β1 integrin partner, is essential for ensuring integrin activation cycle and focal adhesion formation. We show that ICAP-1 is monoubiquitylated by Smurf1, preventing ICAP-1 binding to β1 integrin. The non-ubiquitylatable form of ICAP-1 modifies β1 integrin focal adhesion organization and interferes with fibronectin density sensing. ICAP-1 is also required for adapting cell migration in response to substrate stiffness in a β1-integrin-independent manner. ICAP-1 monoubiquitylation regulates rigidity sensing by increasing MRCKα (also known as CDC42BPA)-dependent cell contractility through myosin phosphorylation independently of substrate rigidity. We provide evidence that ICAP-1 monoubiquitylation helps in switching from ROCK2-mediated to MRCKα-mediated cell contractility. ICAP-1 monoubiquitylation serves as a molecular switch to coordinate extracellular matrix density and rigidity sensing thus acting as a crucial modulator of cell migration and mechanosensing.
Myeloid 12/15-LOX regulates B cell numbers and innate immune antibody levels in vivo
Authors: Lauder Sarah N. , Tyrrell Victoria J , Allen-Redpath Keith , Aldrovandi Maceler , Gray David , Collins Peter , Jones Simon A , Taylor Philip R , O'Donnell Valerie 
Published: January 2017

Background: The myeloid enzyme 12/15-lipoxygenase (LOX), which generates bioactive oxidized lipids, has been implicated in numerous inflammatory diseases, with several studies demonstrating an improvement in pathology in mice lacking the enzyme. However, the ability of 12/15-LOX to directly regulate B cell function has not been studied.Methods: The influence of 12/15-LOX on B cell phenotype and function, and IgM generation, was compared using wildtype (WT) and 12/15-LOX (Alox15-/-) deficient mice. The proliferative and functional capacity of splenic CD19+B cells was measuredin vitroin response to various toll-like receptor agonists.Results: WT andAlox15-/-displayed comparable responses. Howeverin vivo, splenic B cell numbers were significantly elevated inAlox15-/-mice with a corresponding elevation in titres of total IgM in lung, gut and serum, and lower serum IgM directed against the 12/15-LOX product, 12-hydroxyeicosatetraenoic acid-phosphatidylethanolamine (HETE-PE).Discussion: Myeloid 12/15-LOX can regulate B cell numbers and innate immune antibody levelsin vivo, potentially contributing to its ability to regulate inflammatory disease. Furthermore, the alterations seen in 12/15-LOX deficiency likely result from changes in the equilibrium of the immune system that develop from birth. Further studies in disease models are warranted to elucidate the contribution of 12/15-LOX mediated alterations in B cell numbers and innate immune antibody generation to driving inflammationin vivo.
Transient Aortic Intramural Hematoma Complicating Transaortic Valve Replacement
Authors: Thomas Taylor , Poulose Anil , Harris Kevin 
Published: January 2017

AbstractAcute aortic intramural hematoma, which is a variant of acute aortic syndromes, most frequently occurs spontaneously and typically is treated similar to classic aortic dissection. Here, we describe a case in which an iatrogenic aortic intramural hematoma occurs shortly after transaortic valve replacement. The patient was managed conservatively, and the hematoma quickly resolved as demonstrated by serial imaging.
Comparison of the elution properties of commercially available gentamicin and bone cement containing vancomycin with ‘home-made’ preparations
Authors: Frew N. M. , Cannon T. , Nichol T. , Smith T. J. , Stockley I. 
Published: January 2017

Aims Vancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary cement preparations containing vancomycin are available, but are significantly more expensive. We investigated whether the elution of antibiotic from ‘home-made’ cement containing vancomycin was comparable with more expensive commercially available vancomycin impregnated cement. Materials and Methods A total of 18 cement discs containing either proprietary CopalG+V; or ‘home-made’ CopalR+G with vancomycin added by hand, were made. Each disc contained the same amount of antibiotic (0.5 g gentamycin, 2 g vancomycin) and was immersed in ammonium acetate buffer in a sealed container. Fluid from each container was sampled at eight time points over a two-week period. The concentrations of gentamicin and vancomycin in the fluid were analysed using high performance liquid chromatography mass spectrometry. Results The highest peak concentrations of antibiotic were observed from the ‘home-made’ cements containing vancomycin, added as in the operating theatre. The overall elution of antibiotic was, fivefold (vancomycin) and twofold (gentamicin) greater from the ‘home-made’ mix compared with the commercially mixed cement. The use of a vacuum during mixing had no significant effect on antibiotic elution in any of the samples. Conclusion These findings suggest that the addition of 2 g vancomycin powder to gentamicin-impregnated bone cement by hand significantly increases the elution of both antibiotics compared with commercially prepared cements containing vancomycin. We found no significant advantages of using expensive commercially produced vancomycin-impregnated cement and recommend the addition of vancomycin powder by hand in the operating theatre. Cite this article: Bone Joint J 2017;99-B:73–7.
Instability of the hip after anatomical re-alignment in patients with a slipped capital femoral epiphysis
Authors: Aprato A. , Leunig M. , Massé A. , Slongo T. , Ganz R. 
Published: January 2017

Aims Several studies have reported the safety and efficacy of subcapital re-alignment for patients with slipped capital femoral epiphysis (SCFE) using surgical dislocation of the hip and an extended retinacular flap. Instability of the hip and dislocation as a consequence of this surgery has only recently gained attention. We discuss this problem with some illustrative cases. Materials and Methods We explored the literature on the possible pathophysiological causes and surgical steps associated with the risk of post-operative instability and articular damage. In addition, we describe supplementary steps that could be used to avoid these problems. Results The causes of instability may be divided into three main groups: the first includes causes directly related to SCFE (acetabular labral damage, severe abrasion of the acetabular cartilage, flattening of the acetabular roof and a bell-shaped deformity of the epiphysis); the second, causes not related to the SCFE (acetabular orientation and poor quality of the soft tissues); the third, causes directly related to the surgery (capsulotomy, division of the ligamentum teres, shortening of the femoral neck, pelvi-trochanteric impingement, previous proximal femoral osteotomy and post-operative positioning of the leg). Conclusion We present examples drawn from our clinical practice, as well as possible ways of reducing the risks of these complications, and of correcting them if they happen. Cite this article: Bone Joint J 2017;99-B:16–21.
Crossing the line: Drawing as Babel Fish
Authors: Casey Sarah , Davies Gerry 
Published: January 2017

Abstract
This article examines the emergence of illustrative practices among fine artists to achieve a particular mobility, one that enables them to gather, synthesize and communicate information across diverse environments, locations and communities. The article recognizes a growing appetite among contemporary illustrators and artists to work collaboratively and across previously separate disciplines, and focuses on artists leaving the studio to seek out ever more responsive applications of drawing. This reveals a hybrid, fluid approach in drawing, a new sensitivity in which drawing is used by artists as a way of analysing, communicating and reflecting upon aspects of lived experience, some of which might normally be the province of other research professionals. We explore how these ‘itinerant’ artists use drawing to translate into graphic form information, ideas and practices from other fields of activity – for instance, oceanography (Peter Matthews), medicine (Julia Midgley) and political activism (Jill Gibbon). While these contemporary practices are at the cutting edge, we discuss their direct lineage to Ruskin’s Elements of Drawing (1857) and his belief in the use of drawing to interrogate the world and our position in it. We argue that this underacknowledged mode of practice is timely and significant for a globalized interdisciplinary research community because it reveals drawing’s capacity to intercede, for problem-solving and for building relationships across otherwise disparate communities and areas of expertise.
Impact of Asymmetry of Over-Head Power Line Parameters on Short-Circuit Currents
Authors: Eleschová Žaneta , Ivanič Marián 
Published: January 2017

<span lang="EN-GB">This paper analyses the impact of asymmetry of over-head power line parameters on short circuit currents when three-phase fault and phase-to-ground fault occur. The calculation results with consideration of an asymmetry of the power line parameters are confronted with the calculation in accordance with the Slovak standard STN EN 60909 which does not consider asymmetry of equipment parameters in the power system. The calculation of short-circuit conditions was carried out for two types of 400 kV power line towers on which is a considerably different arrangement of phase conductors.</span>
Enzymatic detection of formalin-fixed museum specimens for DNA analysis and enzymatic maceration of formalin-fixed specimens
Authors: Sørensen Margrethe , Rasmussen Arne Redsted , Simonsen Kim Pilkjær 
Published: January 2017

Abstract
A simple enzymatic screening method has been developed to detect whether a tissue sample has been preserved with formalin or with ethanol only because such a method is a useful tool for predicting the quality of genetic test results. The method is based on enzymatic digestion at 55°C at neutral pH. The screening method shows that only ethanol-preserved tissue samples are dissolved, whereas formalin-preserved samples remain undissolved. The method was developed by the incorporation of laboratory rats preserved under controlled conditions in either 4% neutral buffered formalin or 96% ethanol. The method was subsequently tested on wild-living preserved specimens and an archived specimen. The protease enzyme used was Savinase® 16 L, Type EX from Novozymes A/S. The enzymatic screening test demands only simple laboratory equipment. The method is useful for natural history collections in museums where DNA analyses of archived specimens are performed. Wasted time and resources can be avoided through the detection of formalin-fixed specimens because these specimens yield low-quality, damaged DNA. In addition to the screening method, it is shown that formalin-preserved specimens can be macerated by enzymatic digestion under alkaline conditions at 55°C.
Calibrated UV reflectance photography of Hebomoia glaucippe sulphurea
Authors: Ayre Evelyn , Bevan George 
Published: January 2017

Abstract
Ultraviolet (UV) reflective and absorbent markings on wings of male Hebomoia glaucippe sulphurea butterflies are important visual markers used in mating to differentiate them from other species. The objective of our study was to determine whether these markings deteriorate in museum collections over time. We first characterized quantitatively the UV reflective and UV absorbent wing markings from fresh and naturally aged male H. glaucippe sulphurea using UV reflectance microphotography, which was calibrated with handmade reflectance standards. The results of calibrated UV reflectance photography were then compared qualitatively with the same markings using visible light photography, transmitted and reflected visible light microscopy, and scanning electron microscopy (SEM). A UV-converted Nikon D200 with a Baader Ultraviolet Venus lens filter was used to record UV reflective and UV absorbent wing markings of the specimens. The handmade reflectance standards were prepared using magnesium oxide, plaster, and carbon, photographed alongside the specimens, and used to calibrate the photographs. The easily and affordably produced handmade reflectance standards were effective in calibrating the UV reflectance digital photographs, which allowed for each pixel of the digital photographs to be used for optical densitometry measurements. Quantitative data from calibrated UV reflectance photography demonstrated little evidence of deterioration in the UV reflective markings, although there was clear deterioration in the UV absorbent markings. This quantitative data, along with the calibrated UV photographs themselves, offered complementary documentation to visible light microscopy and SEM images. Results show that both visible-spectrum and UV markings fade in naturally aging museum specimens. We conclude that by using calibrated UV reflectance photography, a relatively inexpensive technique, a baseline and eventual degradation of Lepidoptera wing markings may be quantified and may provide valuable data to clarify the mechanisms behind this degradation. With the rate of change quantified, and the mechanisms of fading understood, it is hoped that preventative measures can be taken in the future to remedy this loss of valuable data in collections.