bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sulfation of the Human Cytomegalovirus Protein UL22A Enhances Binding to the Chemokine RANTES
Authors: Wang Xiaoyi , Sanchez Julie , Stone Martin J. , Payne Richard J. 
Published: May 2017

AbstractUL22A is an 83 amino acid chemokine‐binding protein produced by human cytomegalovirus that likely assists the virus in dampening the host antiviral response. We proposed that UL22A is sulfated on two tyrosine residues and tested this hypothesis through the chemical synthesis of a small library of differentially sulfated protein variants. The (sulfo)proteins were efficiently prepared using a novel β‐selenoleucine motif to facilitate one‐pot ligation–deselenization chemistry. Tyrosine sulfation of UL22A proved critical for RANTES binding, with the doubly sulfated variant exhibiting an improvement in binding of 2.5 orders of magnitude compared to the unmodified protein.
Zinc‐Catalyzed Synthesis of Allylsilanes by Si−H Bond Insertion of Vinyl Carbenoids Generated from Cyclopropenes
Authors: Mata Sergio , López Luis A. , Vicente Rubén 
Published: May 2017

AbstractAllylsilanes have long been recognized as valuable building blocks for organic synthesis. A zinc‐catalyzed reaction of cyclopropenes and hydrosilanes provides a convenient route to these versatile unsaturated organosilanes. In this transformation, ZnBr2 serves as an efficient catalyst, allowing the generation of a zinc vinyl carbenoid intermediate, which is subsequently involved in a Si−H bond insertion. The process shows broad scope, and is amenable to substituted and functionalized cyclopropenes or the functionalization of polysiloxanes. Moreover, zinc‐catalyzed carbene insertion into a Ge−H bond is reported for the first time.
Visible‐Light Photocatalytic Intramolecular Cyclopropane Ring Expansion
Authors: Luis‐Barrera Javier , Laina‐Martín Víctor , Rigotti Thomas , Peccati Francesca , Solans‐Monfort Xavier , Sodupe Mariona , Mas‐Ballesté Rubén , Liras Marta , Alemán José 
Published: May 2017

AbstractDescribed herein is a new visible‐light photocatalytic strategy for the synthesis of enantioenriched dihydrofurans and cyclopentenes by an intramolecular nitro cyclopropane ring expansion reaction. Mechanistic studies and DFT calculations are used to elucidate the key factors in this new ring expansion reaction, and the need for the nitro group on the cyclopropane.
Carbonyl Activation in Electrophilic Polyene Cyclizations: A Toolbox for the Design of Isoprenoid Libraries
Authors: Minassi Alberto , Pollastro Federica , Chianese Giuseppina , Caprioglio Diego , Taglialatela‐Scafati Orazio , Appendino Giovanni 
Published: May 2017

AbstractAn approach to biogenetically overlooked areas of the isoprenoid chemical space is presented. This strategy is based on the generation of a cationic center in functionalized polyolefins by Lewis acid activation of a carbonyl group, rather than by electrophilic attack at a double bond. Starting from the monocyclic humulane trienone zerumbone, polycyclic sesquiterpenoid skeletons which are either not reported as natural products or biogenetically enigmatic in terms of the isoprenoid rule, were obtained by modulating the Lewis acid catalyst. In the course of these studies, the surprising formation of a strained E‐cyclooctene motif was observed in a cyclization reaction.
Sorption and desorption properties of random copolymer hydrogels of N‐isopropylacrylamide and N‐ethylacrylamide: Effect of monomer composition
Authors: Biswas Chandra Sekhar , Wang Qiao , Du Bing , Stadler Florian J. 
Published: May 2017

ABSTRACTRandom copolymer hydrogels of N‐isopropylacrylamide and N‐ethylacrylamide (NEAM) were prepared using different monomer compositions in 1:1 methanol–water mixtures. The samples were characterized by cononsolvency study in methanol‐water mixtures at various temperatures, swelling properties measurements, scanning electron microscopy. With changing ratio of the monomers in the reaction mixture, the thermal, morphological and swelling properties, varied significantly. The change in the properties with monomer composition variation are interpreted based on the different thermoresponsive characteristics and interactions of gels of N‐isopropylacrylamide and NEAM (homo‐ and copolymer gels) in water and different methanol‐water mixtures, their variable compositions in the synthesis mixtures, and their morphologies. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45176.
Screening of 181 Patients With Antibody Deficiency for Deficiency of Adenosine Deaminase 2 Sheds New Light on the Disease in Adulthood
Authors: Schepp Johanna , Proietti Michele , Frede Natalie , Buchta Mary , Hübscher Katrin , Rojas Restrepo Jessica , Goldacker Sigune , Warnatz Klaus , Pachlopnik Schmid Jana , Duppenthaler Andrea , Lougaris Vassilios , Uriarte Ignacio , Kelly Susan , Hershfield Michael , Grimbacher Bodo 
Published: May 2017

ObjectiveWe aimed to test the relevance of deficiency of adenosine deaminase 2 (DADA2) in patients with antibody deficiency and describe the clinical picture of the disease in adulthood.MethodsWe screened for DADA2 in a cohort of 181 patients with antibody deficiency with or without vascular lesions using next‐generation sequencing and targeted Sanger sequencing. All mutations were confirmed by determining the ADA2 enzymatic activity levels in dried plasma spots. Clinical data and laboratory values were collected in a standardized format.ResultsFollowing the diagnosis of 2 siblings in the index family, we identified 9 additional affected patients with compound heterozygous or homozygous CECR1 mutations, containing 6 novel and 4 previously published mutations. The patients' age at evaluation ranged from 13 to 51 years, with a median age of 22 years. Clinically, we saw a broad phenotype, ranging from isolated antibody deficiency to recurrent strokes. All but 1 patient had low numbers of memory B cells. Moreover, B cell function seemed to correlate with inflammation.ConclusionTaken together, our findings indicate that DADA2 presents not only with vasculopathy but also with an immunodeficiency of the B cell compartment. Therefore, patients with antibody deficiency should be screened for DADA2. Anti–tumor necrosis factor treatment might improve immunologic features over time and might be considered in patients without vascular manifestations but with elevated inflammation markers. Conservative management has so far proven to be the choice for our less severely affected adolescent and adult DADA2 patients; however, in patients with severe cytopenias and bone marrow failure, hematopoietic stem cell transplantation should be considered.
Early Changes of the Cortical Micro‐Channel System in the Bare Area of the Joints of Patients With Rheumatoid Arthritis
Authors: Werner David , Simon David , Englbrecht Matthias , Stemmler Fabian , Simon Christoph , Berlin Andreas , Haschka Judith , Renner Nina , Buder Thomas , Engelke Klaus , Hueber Axel J. , Rech Jürgen , Schett Georg , Kleyer Arnd 
Published: May 2017

ObjectiveTo characterize the specific structural properties of the erosion‐prone bare area of the human joint, and to search for early microstructural changes in this region during rheumatoid arthritis (RA).MethodsIn the initial part of the study, human cadaveric hand joints were examined for exact localization of the bare area of the metacarpal heads, followed by detection of cortical micro‐channels (CoMiCs) in this region by high‐resolution peripheral quantitative computed tomography (HR‐pQCT) and, after anatomic dissection, validation of the presence of CoMiCs by micro–computed tomography (micro‐CT). In the second part of the study, the number and distribution of CoMiCs were analyzed in 107 RA patients compared to 105 healthy individuals of similar age and sex distribution.ResultsInvestigation by HR‐pQCT combined with adaptive thresholding allowed the detection of CoMiCs in the bare area of human cadaveric joints. The existence of CoMiCs in the bare area was additionally validated by micro‐CT. In healthy individuals, the number of CoMiCs increased with age. RA patients showed significantly more CoMiCs compared to healthy individuals (mean ± SD 112.9 ± 54.7/joint versus 75.2 ± 41.9/joint; P < 0.001), with 20–49‐year‐old RA patients exhibiting similar numbers of CoMiCs as observed in healthy individuals older than age 65 years. Importantly, CoMiCs were already found in RA patients very early in their disease course, with enrichment in the erosion‐prone radial side of the joint.ConclusionCoMiCs represent a new form of structural change in the joints of patients with RA. Although the number of CoMiCs increases with age, RA patients develop CoMiCs much earlier in life, and such changes can even occur at the onset of the disease. CoMiCs therefore represent an interesting new opportunity to assess structural changes in RA.
Distributed Adaptive Event‐Triggered Control for Leader‐Following Consensus of Multi‐Agent Systems
Authors: You Xiu , Hua Changchun , Guan Xinping 
Published: May 2017

AbstractThis paper studies the leader‐following consensus problem for Lipschitz nonlinear multi‐agent systems using novel event‐triggered controllers. A distributed adaptive law is introduced for the event‐based control strategy design such that the proposed controllers are independent of system parameters and only use the relative states of neighboring agents, and hence are fully distributed. Due to the introduction of an event‐triggered control scheme, the controller of the agent is only triggered at it's own event times, and thus reduces the amount of communication between controller and actuator and lowers the frequency of controller updates in practice. Based on a quadratic Lyapunov function, the event condition which uses only neighbor information and local computation at trigger instants is established. Infinite triggers within a finite time are also verified to be impossible. The effectiveness of the theoretical results are illustrated through simulation examples.
Randomized clinical trial of biodegradeable intraluminal sheath to prevent anastomotic leak after stapled colorectal anastomosis
Authors: Bakker I S , Morks A N , ten Cate Hoedemaker H O , Burgerhof J G M , Leuvenink H G , van Praagh J B , Ploeg R J , Havenga K ,   the Collaborative C-seal Study Group, Bakker I S , Morks A N , ten Cate Hoedemaker H O , Leuvenink H G , Ploeg R J , Havenga K , van Etten B , Lange J F M , Hemmer P H J , Burgerhof J G M , Sonneveld D J A , Tanis P J , Wegdam J A , Jonk A , Lutke Holzik M F , Bosker R J I , Lamme B , Spillenaar Bilgen E J , Bremers A J , van der Mijle H C , Hoff C , de Vries D P , Logeman F , Sietses C , Lesanka Versluijs-Ossewaarde F N , Leijtens J W , Tobon Morales R E , Neijenhuis P A , Kloppenberg F W , Schasfoort R , Bleeker W A , Hess D , Rosman C , Wit F , Ton van Engelenburg K C , Pronk A , Bonsing B A , Dekker J W , Consten E C , Patijn G A , Bogdan Rajcs S , Csapó Z , Bálint A , Harsányi L , István G , Horisberger K , Bader F , Kutup A , Mariette C , Cebrián F 
Published: May 2017

Abstract

Background
Anastomotic leakage is a potential major complication after colorectal surgery. The C-seal was developed to help reduce the clinical leakage rate. It is an intraluminal sheath that is stapled proximal to a colorectal anastomosis, covering it intraluminally and thus preventing intestinal leakage in case of anastomotic dehiscence. The C-seal trial was initiated to evaluate the efficacy of the C-seal in reducing anastomotic leakage in stapled colorectal anastomoses.

Methods
This RCT was performed in 41 hospitals in the Netherlands, Germany, France, Hungary and Spain. Patients undergoing elective surgery with a stapled colorectal anastomosis less than 15 cm from the anal verge were eligible. Included patients were randomized to the C-seal and control groups, stratified for centre, anastomotic height and intention to create a defunctioning stoma. Primary outcome was anastomotic leakage requiring invasive treatment.

Results
Between December 2011 and December 2013, 402 patients were included in the trial, 202 in the C-seal group and 200 in the control group. Anastomotic leakage was diagnosed in 31 patients (7·7 per cent), with a 10·4 per cent leak rate in the C-seal group and 5·0 per cent in the control group (P = 0·060). Male sex showed a trend towards a higher leak rate (P = 0·055). Construction of a defunctioning stoma led to a lower leakage rate, although this was not significant (P = 0·095).

Conclusion
C-seal application in stapled colorectal anastomoses does not reduce anastomotic leakage. Registration number: NTR3080 (http://www.trialregister.nl/trialreg/index.asp).
Pipecolic Acid Hydroxylases: A Monophyletic Clade among cis‐Selective Bacterial Proline Hydroxylases that Discriminates l‐Proline
Authors: Mattay Johanna , Hüttel Wolfgang 
Published: May 2017

AbstractProline hydroxylases are iron(II)/2‐oxoglutarate‐dependent enzymes that hydroxylate l‐proline and derivatives, such as lpipecolic acid, which is the six‐membered‐ring homologue of l‐proline. It has been established that there is a distinct group of conserved bacterial enzymes that hydroxylate l‐pipecolic acid and trans‐3‐ and trans‐4‐methyl‐l‐proline, but virtually no l‐proline. This allows the organism to produce hydroxyproline congeners without hydroxylation of the physiologically omnipresent l‐proline. In vitro conversions showed that the substrate spectrum of the pipecolic acid hydroxylases GetF (from a Streptomyces sp.; producer of the tetrapeptide antibiotic GE81112) and PiFa (from Frankia alni) overlaps that of proline hydroxylases, except for the nonacceptance of l‐proline and smaller homologues. Distinct and conserved residues were determined for both types of enzymes. However, site‐directed mutagenesis in GetF did not yield variants that accepted l‐proline; this suggested a complex interaction of several residues around the active site, which resulted in delicate changes in substrate specificity. This is supported by substrate docking in a homology model of GetF, which revealed an altered orientation for l‐proline relative to that of preferred substrates.
From Active‐Site Models to Real Catalysts: Importance of the Material Gap in the Design of Pd Catalysts for Methane Oxidation
Authors: Doan Hieu A. , Sharma Munish K. , Epling William S. , Grabow Lars C. 
Published: May 2017

AbstractThe front cover artwork for Issue 9/2017 is provided by the University of Houston. The image shows the charge density difference in the transition state of methane activation over a single crystal Pd(1 0 0) surface as obtained from electronic structure simulations. See the Full Paper itself at https://doi.org/10.1002/cctc.201601333.
Unsaturated Long‐Chain Fatty Acids Are Preferred Ferritin Ligands That Enhance Iron Biomineralization
Authors: Zanzoni Serena , Pagano Katiuscia , D'Onofrio Mariapina , Assfalg Michael , Ciambellotti Silvia , Bernacchioni Caterina , Turano Paola , Aime Silvio , Ragona Laura , Molinari Henriette 
Published: May 2017

AbstractFerritin is a ubiquitous nanocage protein, which can accommodate up to thousands of iron atoms inside its cavity. Aside from its iron storage function, a new role as a fatty acid binder has been proposed for this protein. The interaction of apo horse spleen ferritin (HoSF) with a variety of lipids has been here investigated through NMR spectroscopic ligand‐based experiments, to provide new insights into the mechanism of ferritin–lipid interactions, and the link with iron mineralization. 1D 1H, diffusion (DOSY) and saturation‐transfer difference (STD) NMR experiments provided evidence for a stronger interaction of ferritin with unsaturated fatty acids compared to saturated fatty acids, detergents, and bile acids. Mineralization assays showed that oleate c aused the most efficient increase in the initial rate of iron oxidation, and the highest formation of ferric species in HoSF. The comprehension of the factors inducing a faster biomineralization is an issue of the utmost importance, given the association of ferritin levels with metabolic syndromes, such as insulin resistance and diabetes, characterized by fatty acid concentration dysregulation. The human ferritin H‐chain homopolymer (HuHF), featuring ferroxidase activity, was also tested for its fatty acid binding capabilities. Assays show that oleate can bind with high affinity to HuHF, without altering the reaction rates at the ferroxidase site.
Asymmetric Synthesis of β‐Lactams through Copper‐Catalyzed Alkyne–Nitrone Coupling with a Prolinol–Phosphine Chiral Ligand
Authors: Takayama Yurie , Ishii Takaoki , Ohmiya Hirohisa , Iwai Tomohiro , Schwarzer Martin C. , Mori Seiji , Taniguchi Tohru , Monde Kenji , Sawamura Masaya 
Published: May 2017

AbstractProlinol–phosphine chiral ligands enabled highly enantioselective copper‐catalyzed intermolecular alkyne–nitrone coupling (Kinugasa reaction) to produce 1,3,4‐trisubstituted chiral β‐lactams. A high level of enantiocontrol was achieved not only with aryl‐ or alkenylacetylenes but also with alkylacetylenes, which were important but unfavorable substrates in the previously reported protocols. Two‐point hydrogen bonding between the chiral ligand and the nitrone oxyanion consisting of O−H⋅⋅⋅O and C(sp3)−H⋅⋅⋅O hydrogen bonds is proposed.
Plasmonic Au Decorated Single‐crystal‐like TiO2‐NaYF4 Mesoporous Microspheres for Enhanced Broadband Photocatalysis
Authors: Wang Yuanwei , Zhu Yihua , Yang Xiaoling , Shen Jianhua , Zhu Jingrun , Qian Shaohong , Li Chunzhong 
Published: May 2017

AbstractSingle‐crystal‐like TiO2 mesoporous microspheres have been reported with high photocatalytic activity under ultraviolet light (UV light) because of their high specific surface areas and single‐crystal‐like channel walls. In this work, plasmonic gold nanoparticles (Au NPs) and β‐NaYF4: Yb3+, Er3+ upconversion nanoparticles (UCNPs) were composited with single‐crystal‐like TiO2 mesoporous microspheres through a series of facile approaches, aiming at broadening response region of solar light from UV to visible and near infrared light and enhancing the photocatalytic activity further. The structure was rationally designed by modifying the pore size of TiO2 mesoporous microspheres so as to anchor plasmonic Au NPs, and covering β‐NaYF4: Yb3+, Er3+ with SiO2 in order to embed UCNPs into TiO2 mesoporous microspheres via hydrophilic interaction. This work studied the attribution of Au NPs and UCNPs to photocatalysis and found out that combining Au NPs and certain amount of UCNPs with single‐crystal‐ like TiO2 mesoporous microspheres in a monolithic architecture would bring enhanced broadband photocatalytic activity under simulated solar light. Consequently, the composite photocatalyts containing 150 mg UCNPs showed a significant enhancement in reaction rate, which was 36.02% higher than commercial P25 and 85.09% higher than pure TiO2 mesoporous microspheres under simulated solar light.
Neladenoson Bialanate Hydrochloride: A Prodrug of a Partial Adenosine A1 Receptor Agonist for the Chronic Treatment of Heart Diseases
Authors: Meibom Daniel , Albrecht‐Küpper Barbara , Diedrichs Nicole , Hübsch Walter , Kast Raimund , Krämer Thomas , Krenz Ursula , Lerchen Hans‐Georg , Mittendorf Joachim , Nell Peter G. , Süssmeier Frank , Vakalopoulos Alexandros , Zimmermann Katja 
Published: May 2017

AbstractAdenosine is known to be released under a variety of physiological and pathophysiological conditions to facilitate the protection and regeneration of injured ischemic tissues. The activation of myocardial adenosine A1 receptors (A1Rs) has been shown to inhibit myocardial pathologies associated with ischemia and reperfusion injury, suggesting several options for new cardiovascular therapies. When full A1R agonists are used, the desired protective and regenerative cardiovascular effects are usually overshadowed by unintended pharmacological effects such as induction of bradycardia, atrioventricular (AV) blocks, and sedation. These unwanted effects can be overcome by using partial A1R agonists. Starting from previously reported capadenoson we evaluated options to tailor A1R agonists to a specific partiality range, thereby optimizing the therapeutic window. This led to the identification of the potent and selective agonist neladenoson, which shows the desired partial response on the A1R, resulting in cardioprotection without sedative effects or cardiac AV blocks. To circumvent solubility and formulation issues for neladenoson, a prodrug approach was pursued. The dipeptide ester neladenoson bialanate hydrochloride showed significantly improved solubility and exposure after oral administration. Neladenoson bialanate hydrochloride is currently being evaluated in clinical trials for the treatment of heart failure.
Comprehensive analysis of cutaneous T‐cell lymphoma (CTCL) incidence and mortality in Canada reveals changing trends and geographic clustering for this malignancy
Authors: Ghazawi Feras M. , Netchiporouk Elena , Rahme Elham , Tsang Matthew , Moreau Linda , Glassman Steven , Provost Nathalie , Gilbert Martin , Jean Sara‐Elizabeth , Pehr Kevin , Sasseville Denis , Litvinov Ivan V. 
Published: May 2017

BACKGROUNDPrevious reports of geographic clustering of cutaneous T‐cell lymphoma (CTCL) in Texas, Pittsburgh, and Sweden as well as the occurrence of CTCL in married couples and family members raise a possibility of the existence of an external and potentially preventable trigger(s) for this rare skin cancer.METHODSThe authors studied CTCL incidence and mortality in Canada using 3 distinct population‐based cancer databases. Data on patients' sex, age at the time of diagnosis, subtype of CTCL malignancy, reporting province, city, and postal code were analyzed. CTCL cases were mapped across Canada using geographic information systems software.RESULTSIn total, 6685 patients with CTCL were identified in Canada during 1992 through 2010 (CTCL incidence rate, 11.32 cases per million individuals per year), of which 58% were males. The mean age at diagnosis was 59.4 ± 21.5 years. Geographic analysis of patients revealed increased CTCL incidence on the provincial and city levels in several eastern provinces and in Manitoba. An analysis according to postal codes (Forward Sortation Area [FSA]) identified select communities in which several high‐incidence FSAs were contiguous or adjacent. Several of these FSAs were located in industrial regions of Canadian cities. Conversely, 3 of 8 low‐incidence FSAs were clustered in Ottawa, Ontario, which has very little industrial presence. An analysis of CTCL mortality in Canada corroborated the current incidence findings.CONCLUSIONSThe current results provide a comprehensive analysis of CTCL burden in Canada and highlight several important areas of geographic case clustering. These findings argue that industrial exposures may play an important role in promoting CTCL pathogenesis. Cancer 2017;123:3550‐67. © 2017 American Cancer Society.