bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global quantification of phosphoproteins combining metabolic labeling and gel‐based proteomics in B. pumilus
Authors: Hentschker Christian , Dewald Carolin , Otto Andreas , Büttner Knut , Hecker Michael , Becher Dörte 
Published: September 2017

AbstractDifferential proteomics targeting the protein abundance is commonly used to follow changes in biological systems. Differences in localization and degree of post‐translational modifications of proteins including phosphorylations are of tremendous interest due to the anticipated role in molecular regulatory processes. Because of their particular low abundance in prokaryotes, identification and quantification of protein phosphorylation is traditionally performed by either comparison of spot intensities on two‐dimensional gels after differential phosphoprotein staining or gel‐free by stable isotope labeling, sequential phosphopeptide enrichment and following LC‐MS analysis. In the current work, we combined in a proof‐of‐principle experiment these techniques using 14N/15N metabolic labeling with succeeding protein separation on 2D gels. The visualization of phosphorylations on protein level by differential staining was followed by protein identification and determination of phosphorylation sites and quantification by LC‐MS/MS. This approach should avoid disadvantages of traditional workflows, in particular the limited capability of peptide‐based gel‐free methods to quantify isoforms of proteins. Comparing control and stress conditions allowed for relative quantification in protein phosphorylation in Bacillus pumilus exposed to hydrogen peroxide. Altogether, we quantified with this method 19 putatively phosphorylated proteins.
In vivo study of the effect of lactoferrin on iron metabolism and bioavailability from different iron chemical species for formula milk fortification
Authors: Fernández‐Menéndez Sonia , Fernández‐Sánchez María L. , Alves Peixoto Rafaella Regina , Fernández‐Colomer Belén , Sanz‐Medel Alfredo 
Published: September 2017

AbstractIron fortification in infant formulas is a common practice for providing iron to newborns in order to avoid its deficiency (anemia). Depending on the physicochemical species used, its bioavailability might be insufficient to meet iron requirements. In this vein, the influence of Lactoferrin (Lf) presence on iron bioavailability in 2‐week‐old wistar rats fed with formula milk fortified with 57Fe(III)2‐Lf or 57Fe(II)SO4 (in presence of Lf) using quantitative speciation (by HPLC‐ICP‐MS) and Isotope Pattern Deconvolution (IPD) is studied here. Results obtained were compared among fortifiers and also with the maternal group. In RBCs, iron was mainly bound to hemoglobin in all the assayed groups in the same extent. Regarding serum samples, several iron‐proteins were observed (such as transferrin and albumin). In both samples, iron content in the fractions studied was similar in all groups compared and exogenous 57Fe incorporation of intaked iron was always above 50%, showing no significative differences between physicochemical forms but related to the dose administered. Regarding iron stores (liver) the group fed with formula milk fortified with the higher dose of 57FeSO4 in presence of Lf presented the highest values of total iron even superior than those found in the maternal group, and also the highest exogenous (57Fe) incorporation. In conclusion, it was proved that iron fortification is required to ensure proper iron levels in all body compartments. No significative differences were observed between different physicochemical species when iron is administered at low doses. However, higher iron doses lead to a greater incorporation in all the iron‐proteins studied.
A rapid azo dye decolorization of methyl orange by the foam zero‐valent nickel
Authors: Deng Feng‐Xia , Yang Ji‐Xian , Zhu Ying‐Shi , Ma Fang , Qiu Shan 
Published: September 2017

In this article, foam zero‐valent nickel (FZVN) was put forward as a promising zero‐valent metal for the pretreatment of azo dye wastewater (MO as a model contaminant). The characteristics of FZVN were evaluated by methods of Barrett‐Emmett‐Teller, scanning electron microscopy, X‐ray diffraction, and X‐ray photoelectron spectroscopy. Moreover, influence of key experimental parameters, including oxygen concentration, presence of different acid, pH, and temperature on the MO decolorization were systematically ascertained. Furthermore, possible mechanism of MO removal by FZVN was investigated by using Liquid chromatography‐mass spectrometry (LC‐MS). Results showed that FZVN was a three‐dimensional pore material with a specific surface area of 3 m2 g−1. Moreover, anoxic condition, presence of acetic acid, higher temperatures and acid conditions were beneficial to decolorize MO by FZVN while the decolorization process obeyed the first order kinetic model with an apparent rate constant (kapp) of 0.162 min−1 at pH value of 4 (25°C). The activation energy Ea obtained from the decolorization reaction was 83.1 kJ mol−1. Based on LC‐MS results, the role of FZVN in the decolorization reaction process was suggested as follows: strong reductive, catalysis, and adsorption. © 2017 American Institute of Chemical Engineers Environ Prog, 37: 686–694, 2018
Sensitivity for Cues Predicting Reward and Punishment in Young Women with Eating Disorders
Authors: Matton Annelies , de Jong Peter , Goossens Lien , Jonker Nienke , Van Malderen Eva , Vervaet Myriam , De Schryver Nele , Braet Caroline 
Published: September 2017

AbstractIncreasing evidence shows that sensitivity to reward (SR) and punishment (SP) may be involved in eating disorders (EDs). Most studies used self‐reported positive/negative effect in rewarding/punishing situations, whereas the implied proneness to detect signals of reward/punishment is largely ignored. This pilot study used a spatial orientation task to examine transdiagnostic and interdiagnostic differences in SR/SP. Participants (14–29 years) were patients with anorexia nervosa of restricting type (AN‐R, n = 20), binge/purge ED group [AN of binge/purge type and bulimia nervosa (n = 16)] and non‐symptomatic individuals (n = 23). Results revealed stronger difficulties to redirect attention away from signals of rewards in AN‐R compared with binge/purge EDs, and binge/purge EDs showed stronger difficulties to direct attention away from signals of punishment compared with AN‐R. Findings demonstrate interdiagnostic differences and show that the spatial orientation task is sensitive for individual differences in SP/SR within the context of EDs, thereby sustaining its usefulness as behavioural measure of reinforcement sensitivity. Copyright © 2017 John Wiley & Sons, Ltd and Eating Disorders Association.
Psychophysiological Changes during Pure vs Guided Mirror Exposure Therapies in Women with High Body Dissatisfaction: What Are They Learning about Their Bodies?
Authors: Díaz‐Ferrer Sandra , Rodríguez‐Ruiz Sonia , Ortega‐Roldán Blanca , Mata‐Martín José Luis , Carmen Fernández‐Santaella M. 
Published: September 2017

AbstractThis study aimed to examine the psychophysiological changes resulting from two mirror exposure treatments that are effective at reducing body dissatisfaction. Thirty‐five university women with body dissatisfaction and subclinical eating disorders were randomly assigned to one of two groups: pure (n = 17) or guided exposure (n = 18). The participants received six sessions of treatment. Their thoughts, feelings and avoidance behaviours were assessed after each session. Their subjective discomfort, heart rate and skin conductance were assessed within the sessions. Both groups showed improvement in cognitive‐affective and avoidance behaviour symptoms. Nevertheless, the pure exposure group showed faster habituation of subjective discomfort and a greater physiological response than the guided exposure group. These findings suggest that both procedures are effective interventions for improving body image disturbances, although psychophysiological changes observed within session suggest that each technique would act through different processes. Copyright © 2017 John Wiley & Sons, Ltd and Eating Disorders Association.
Divide‐and‐allocate: An uplink successive bandwidth division NOMA system
Authors: Qureshi Soma , Hassan Syed Ali , Jayakody Dushantha Nalin K. 
Published: September 2017

AbstractThis paper investigates a new spectrum‐sharing strategy using non‐orthogonal multiple access (NOMA), a technique that is expected to lead toward the fifth generation of wireless networks. The proposed scheme, namely, successive bandwidth division (SBD), NOMA, is a hybrid approach exploiting the characteristics of both the conventional NOMA system and the orthogonal multiple access (OMA) system. Power allocation is being performed in successive bandwidth division NOMA to maximize the sum rate using a divide‐and‐allocate approach such that all users are allocated with optimal transmission power. Under Rayleigh fading, the probability density function (PDF) of the received signal‐to‐interference‐plus‐noise ratio (SINR) is derived, and closed‐form expressions for the outage probability are presented when channel state information (CSI) is available at the base station. The performance evaluation is carried out in terms of receiver complexity, average sum rate, and outage probability. Simulations results are provided to access and compare the performance of the proposed scheme with other contemporary approaches.
Effects of maltodextrins with different dextrose‐equivalent values
Authors: Lee Sang Mi , Cho Ar Reum , Yoo Sang‐Ho , Kim Young‐Suk 
Published: September 2017

AbstractThe release of aldehydes from aqueous model systems containing starch or maltodextrins with different dextrose‐equivalent (DE) values was evaluated using static headspace analysis. The patterns of aldehydes released differed significantly between starch and maltodextrin solutions with DE values of 4.0–7.0, 13.0–17.0, and 16.5–19.5. The release of most of aldehydes showed an increasing tendency according to DE values of maltodextrins. The release of most aldehydes decreased up to around 24 h, but then increased slightly at 48 h. In addition, the absorbance values of iodine‐polysaccharide complexes and the particle sizes of the polysaccharides were measured to investigate the effects of hydrophobic regions and the intermolecular aggregation of polysaccharides on the releases of aldehydes. The absorbance values of iodine‐polysaccharide complexes, which could be related to the internal hydrophobic regions, decreased in the maltodextrin and starch solutions as the equilibrium time increased up to 24 h, but then increased at 48 h. Also, the particle size increased gradually to 48 h, suggesting that the intermolecular aggregation of polysaccharides affects the release of aldehydes.
Mineralogy and chemical aspects of some ophiolitic metaultramafics, central Eastern Desert, Egypt: Evidences from chromites, sulphides and gangues
Authors: Abdel‐Karim Abdel‐Aal M. , El‐Shafei Shaimaa A. 
Published: September 2017

Chromites and sulphides are the most common ore minerals in the ophiolitic metasomatized ultramafics (~790 Ma) of Um Halham, Fawakhir and Barramiya, central Eastern Desert, Egypt. These ultramafics exhibit variable degrees of alteration and metasomatism and include massive serpentinites (serpentinized peridotite and serpentinite), tremolite‐talc rocks, talc‐carbonate rocks, listwaenite‐like rocks and typical listwaenite.The alteration of chromite to Cr‐magnetite was accompanied by the formation of chloritic aureoles due to the release of Al and Mg from chromite. Textural and compositional features of the chromites suggest a greenschist up to lower amphibolite facies metamorphism (at 500–600 °C), which is facial with the host ultramafics. The chromites exhibit a boninitic affinity and a forearc suprasubduction setting of the present ophiolite assemblages. The variability of chromite chemistry indicates a melt‐rock reaction, together with the water‐bearing melt which is necessary for crystallizing chromite. Pentlandite, pyrrhotite, arsenopyrite, gersdorffite and pyrite are the most common sulphide minerals. Gold occurs either as an invisible phase associated with gersdorffite, arsenopyrite, and As‐rich pyrite or as native nuggets in listwaenites of Barramiya area. Gangue metasomatized phases associated with these ore minerals are identified as sericite, carbonate and chlorite. The investigated sericite ranges in composition from mariposite (>0.5% Cr) to fuchsite (>1% Cr), which indicates typical listwaenites. Five varieties of carbonate minerals are identified; magnesite, breunnerite, dolomite, calcite and ankerite.The CO2‐rich fluids are released during progressive decarbonation reactions in carbonate‐bearing metasediments, whereas Ni and As‐rich fluids are attributed to nearby granitic intrusions affected on serpentinites and leached Ni and precious metals like gold forming As‐Ni‐rich fluids. These metasomatic fluids are thought to reflect a range of melts derived from a compositionally evolving source during subduction initiation in a forearc environment.
Dorsal striatum mediates deliberate decision making, not late‐stage, stimulus–response learning
Authors: Hiebert Nole M. , Owen Adrian M. , Seergobin Ken N. , MacDonald Penny A. 
Published: September 2017

AbstractWe investigated a controversy regarding the role of the dorsal striatum (DS) in deliberate decision‐making versus late‐stage, stimulus–response learning to the point of automatization. Participants learned to associate abstract images with right or left button presses explicitly before strengthening these associations through stimulus–response trials with (i.e., Session 1) and without (i.e., Session 2) feedback. In Session 1, trials were divided into response‐selection and feedback events to separately assess decision versus learning processes. Session 3 evaluated stimulus–response automaticity using a location Stroop task. DS activity correlated with response‐selection and not feedback events in Phase 1 (i.e., Blocks 1–3), Session 1. Longer response times (RTs), lower accuracy, and greater intertrial variability characterized Phase 1, suggesting deliberation. DS activity extinguished in Phase 2 (i.e., Blocks 4–12), Session 1, once RTs, response variability, and accuracy stabilized, though stimulus–response automatization continued. This was signaled by persisting improvements in RT and accuracy into Session 2. Distraction between Sessions 1 and 2 briefly reintroduced response uncertainty, and correspondingly, significant DS activity reappeared in Block 1 of Session 2 only. Once stimulus–response associations were again refamiliarized and deliberation unnecessary, DS activation disappeared for Blocks 2–8, Session 2. Interference from previously learned right or left button responses with incongruent location judgments in a location Stroop task provided evidence that automaticity of stimulus–specific button‐press responses had developed by the end of Session 2. These results suggest that DS mediates decision making and not late‐stage learning, reconciling two, independently evolving and well‐supported literatures that implicate DS in different cognitive functions. Hum Brain Mapp 38:6133–6156, 2017. © 2017 Wiley Periodicals, Inc.
Upregulation of pAKT(Ser473) expression in progression of HPV‐positive oropharyngeal squamous cell carcinoma
Authors: Horn Dominik , Freudlsperger Christian , Holzinger Dana , Kunzmann Kevin , Plinkert Peter , Dyckhoff Gerhard , Hoffmann Jürgen , Freier Kolja , Hess Jochen 
Published: September 2017

AbstractBackgroundPIK3CA alterations have been shown to be a frequent event in oropharyngeal squamous cell cancer (SCC), especially in human papillomavirus (HPV)‐related tumors.MethodsTissue microarrays (TMAs) were used to evaluate pAKT(Ser473)/(Thr308), total protein kinase B (AKT)(pan) and phosphatase and tensin homolog (PTEN) expression in primary tumors and corresponding nodal disease in oropharyngeal SCC. The HPV status was determined in regard of HPV16 DNA and RNA. Survival analysis was performed by using Kaplan‐Meier curves, log‐rank testing, and multivariate Cox regression analysis.ResultsHPV16 is a prognostic predictive marker for advanced oropharyngeal SCC. pAKT(Ser473) and PTEN are highly expressed in HPV‐related oropharyngeal SCCs in contrast to pAKT(Thr308). The pAKT(Ser473) expression increased from primary tumors to progressive nodal disease (21.1%; P < .011).ConclusionActivation of phosphoinositide 3‐kinase (PI3K)/pAKT(Ser473) frequently occurs in advanced HPV‐positive oropharyngeal SCC and elevated pAKT(Ser473) levels represent a feature during progression of oropharyngeal SCC, indicating a critical role of the mammalian target of rapamycin (mTOR) complex. Further studies are required to evaluate specific drugs targeting PI3K/AKT/mTOR in consideration of PIK3CA alterations.
Prognostic biomarkers in patients with human immunodeficiency virus‐positive disease with head and neck squamous cell carcinoma
Authors: Zhang Hongzheng , Kim Sungjin , Chen Zhengjia , Nannapaneni Sreenivas , Chen Amy Y. , Moore Charles E. , Sica Gabriel , Mosunjac Marina , Nguyen Minh Ly T. , D'Souza Gypsyamber , Carey Thomas E. , Peterson Lisa A. , McHugh Jonathan B. , Graham Martin , Komarck Christine M. , Wolf Gregory T. , Walline Heather M. , Bellile Emily , Riddell James , Pai Sara I. , Sidransky David , Westra William H. , William William N. , Lee J. Jack , El‐Naggar Adel K. , Ferris Robert L. , Seethala Raja , Grandis Jennifer R. , Chen Zhuo Georgia , Saba Nabil F. , Shin Dong M. ,   on behalf of the Head and Neck Cancer SPORE HIV supplement consortium
Published: September 2017

AbstractBackgroundWe examined the prognostic value of a panel of biomarkers in patients with squamous cell carcinoma of the head and neck (SCCHN) who were human immunodeficiency virus (HIV) positive (HIV‐positive head and neck cancer) and HIV negative (HIV‐negative head and neck cancer).MethodsTissue microarrays (TMAs) were constructed using tumors from 41 disease site‐matched and age‐matched HIV‐positive head and neck cancer cases and 44 HIV‐negative head and neck cancer controls. Expression of tumor biomarkers was assessed by immunohistochemistry (IHC) and correlations examined with clinical variables.ResultsExpression levels of the studied oncogenic and inflammatory tumor biomarkers were not differentially regulated by HIV status. Among patients with HIV‐positive head and neck cancer, laryngeal disease site (P = .003) and Clavien‐Dindo classification IV (CD4) counts <200 cells/μL (P = .01) were associated with poor prognosis. Multivariate analysis showed that p16 positivity was associated with improved overall survival (OS; P < .001) whereas increased expression of transforming growth factor‐beta (TGF‐β) was associated with poor clinical outcome (P = .001).ConclusionDisease site has significant effect on the expression of biomarkers. Expression of tumor TGF‐β could be a valuable addition to the conventional risk stratification equation for improving head and neck cancer disease management strategies.
Lake flooding sensitivity to the relative timing of peak flows between upstream and downstream waterways: A case study of Poyang Lake, China
Authors: Li Xianghu , Zhang Qi , Hu Qi , Zhang Dan , Ye Xuchun 
Published: September 2017

AbstractThe relative timing of peak flows (RTPF) from tributaries has significant influence on flood occurrence at their confluence. This study is aimed at (1) analysing the characteristics of the RTPF of the 5 recharging rivers in the Poyang Lake catchment and the Yangtze River during the period of 1960–2012, and (2) employing a physically‐based hydrodynamic model (MIKE 21) to quantify the effects of RTPF on flood behaviour in the Poyang Lake (the largest freshwater lake in China). The results show that short RTPF, or close occurrence of peak flows, triggers flood in the Poyang Lake more easily. More than 75% of total flood events in the study period occurred with RTPF less than 60 days, and more than 55% of the events occurred with RTPF less than 30 days. The hydrodynamic simulation revealed that the date of flood peak in the lake was postponed by 4–7 days and the flood stage raised by 0.69 m because of the delay of peak flows from the upstream rivers/tributaries. On the other hand, earlier start of the Yangtze River peak flow led to flood peak in the lake 6–13 days earlier. Additionally, the duration of high lake water levels was extended by 9–12 days when the RTPF shortened, and the flood hydrograph of the Poyang Lake changed from a flat to a flashy type. These results indicate that an enlarged RTPF between the upstream rivers and the Yangtze River could be an effective way to prevent flood disasters in the Poyang Lake, a method apparently being adopted in the operation of the Three Gorges Dam. The RTPF should be considered and integrated when developing flood prevention and management plans in the Poyang Lake, as well as in other similar regions in the world.
Insights into Activation of Cobalt Pre‐Catalysts for C(sp2)−H Functionalization
Authors: Obligacion Jennifer V. , Zhong Hongyu , Chirik Paul J. 
Published: September 2017

AbstractThe activation of readily prepared, air‐stable cobalt(II) bis(carboxylate) pre‐catalysts for the functionalization of C(sp2)−H bonds has been systematically studied. With the pyridine bis(phosphine) chelate, iPrPNP, treatment of 1‐(O2CtBu)2 with either B2Pin2 or HBPin generated cobalt boryl products. With the former, reduction to (iPrPNP)CoIBPin was observed while with the latter, oxidation to the cobalt(III) dihydride boryl, trans‐(iPrPNP)Co(H)2BPin occurred. The catalytically inactive cobalt complex, Co[PinB(O2CtBu)2]2, accompanied formation of the cobalt‐boryl products in both cases. These results demonstrate that the pre‐catalyst activation from cobalt(II) bis(carboxylates), although effective and utilizes an air‐stable precursor, is less efficient than activation of cobalt(I) alkyl or cobalt(III) dihydride boryl complexes, which are quantitatively converted to the catalytically relevant cobalt(I) boryl. Related cobalt(III) dihydride silyl and cobalt(I) silyl complexes were also synthesized from treatment of trans‐(iPrPNP)Co(H)2BPin and (iPrPNP)CoPh with HSi(OEt)3, respectively. No catalytic silylation of arenes was observed with either complex likely due to the kinetic preference for reversible C−H reductive elimination rather than product‐ forming C−Si bond formation from cobalt(III). Syntheses of the cobalt(II) bis(carboxylate) and cobalt(I) alkyl of iPrPONOP, a pincer where the methylene spacers have been replaced by oxygen atoms, were unsuccessful due to deleterious P−O bond cleavage of the pincer. Despite their structural similarity, the rich catalytic chemistry of iPrPNP was not translated to iPrPONOP due to the inability to access stable cobalt precursors as a result of ligand decomposition via P−O bond cleavage.
The global therapist competence scale for youth psychosocial treatment: Development and initial validation
Authors: Brown Ruth C. , Southam‐Gerow Michael A. , McLeod Bryce D. , Wheat Emily B. , Tully Carrie B. , Reise Steven P. , Kendall Philip C. , Weisz John R. 
Published: September 2017

AbstractObjectiveWe describe the development and initial psychometric properties of the observer‐rated Global Therapist Competence Scale for Youth Psychosocial Treatment (G‐COMP) in the context of cognitive‐behavioral treatment (CBT) for youth anxiety disorders.MethodIndependent coders rated 744 sessions from a sample of 68 youth (mean age = 10.56 years) using the G‐COMP and the instruments of alliance, involvement, CBT adherence, CBT competence.ResultsInter‐rater reliability coefficients, ICC(2,2), were greater than .60 for the 5 G‐COMP domain scores. G‐COMP scores yielded small to medium correlations with instruments of alliance (rs = .17–.44) and youth involvement in treatment (rs = .08–.53), and medium to large correlations with instruments of CBT competence and adherence (rs = .26–.63). Therapists in the research setting were rated higher compared to newly trained therapists in community clinics.ConclusionPreliminary reliability and validity of the G‐COMP are promising, but future research is needed with non‐CBT samples.