bluuebunny/crossref_metadata_2025_split huggingface dataset:
CCL2 Produced by the Glioma Microenvironment Is Essential for the Recruitment of Regulatory T Cells and Myeloid-Derived Suppressor Cells
Authors: Chang Alan L. , Miska Jason , Wainwright Derek A. , Dey Mahua , Rivetta Claudia V. , Yu Dou , Kanojia Deepak , Pituch Katarzyna C. , Qiao Jian , Pytel Peter , Han Yu , Wu Meijing , Zhang Lingjiao , Horbinski Craig M. , Ahmed Atique U. , Lesniak Maciej S. 
Published: August 2016

Abstract
In many aggressive cancers, such as glioblastoma multiforme, progression is enabled by local immunosuppression driven by the accumulation of regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC). However, the mechanistic details of how Tregs and MDSCs are recruited in various tumors are not yet well understood. Here we report that macrophages and microglia within the glioma microenvironment produce CCL2, a chemokine that is critical for recruiting both CCR4+ Treg and CCR2+Ly-6C+ monocytic MDSCs in this disease setting. In murine gliomas, we established novel roles for tumor-derived CCL20 and osteoprotegerin in inducing CCL2 production from macrophages and microglia. Tumors grown in CCL2-deficient mice failed to maximally accrue Tregs and monocytic MDSCs. In mixed-bone marrow chimera assays, we found that CCR4-deficient Treg and CCR2-deficient monocytic MDSCs were defective in glioma accumulation. Furthermore, administration of a small-molecule antagonist of CCR4 improved median survival in the model. In clinical specimens of glioblastoma multiforme, elevated levels of CCL2 expression correlated with reduced overall survival of patients. Finally, we found that CD163-positive infiltrating macrophages were a major source of CCL2 in glioblastoma multiforme patients. Collectively, our findings show how glioma cells influence the tumor microenvironment to recruit potent effectors of immunosuppression that drive progression. Cancer Res; 76(19); 5671–82. ©2016 AACR.
Plasma 25-Hydroxyvitamin D and Risk of Breast Cancer in Women Followed over 20 Years
Authors: Eliassen A. Heather , Warner Erica T. , Rosner Bernard , Collins Laura C. , Beck Andrew H. , Quintana Liza M. , Tamimi Rulla M. , Hankinson Susan E. 
Published: August 2016

Abstract
Experimental evidence supports a protective role of 25-hydroxyvitamin D [25(OH)D] in breast carcinogenesis, but epidemiologic evidence is inconsistent. Whether plasma 25(OH)D interacts with breast tumor expression of vitamin D receptor (VDR) and retinoid X receptor-α (RXR) has not been investigated. We conducted a nested case–control study in the Nurses' Health Study, with 1,506 invasive breast cancer cases diagnosed after blood donation in 1989–1990, 417 of whom donated a second sample in 2000–2002. VDR and RXR expression were assessed by immunohistochemical staining of tumor microarrays (n = 669 cases). Multivariate relative risks (RR) and 95% confidence intervals (CI) were calculated using conditional logistic regression. Plasma 25(OH)D levels were not associated with breast cancer risk overall [top (≥32.7 ng/mL) vs. bottom (&lt;17.2 ng/mL) quintile RR = 0.87; 95% CI, 0.67–1.13; P trend = 0.21]. 25(OH)D measured in summer (May–October) was significantly inversely associated with risk (top vs. bottom quintile RR = 0.66; 95% CI, 0.46–0.94; P trend = 0.01); winter levels (November–April) were not (RR = 1.10; 95% CI, 0.75–1.60; P trend = 0.64; P interaction = 0.03). 25(OH)D levels were inversely associated with risk of tumors with high expression of stromal nuclear VDR [≥30 ng/mL vs. &lt;30 ng/mL RR (95% CI): VDR ≥ median = 0.67 (0.48–0.93); VDR &lt; median = 0.98 (0.72–1.35), P heterogeneity = 0.12] and significantly stronger for summer measures (P heterogeneity = 0.01). Associations were not significantly different by RXR expression. No overall association was observed between plasma 25(OH)D and breast cancer risk. However, our results suggest women with high, compared with low, plasma 25(OH)D levels in the summer have a reduced breast cancer risk, and plasma 25(OH)D may be inversely associated with risk of tumors expressing high levels of VDR. Cancer Res; 76(18); 5423–30. ©2016 AACR.
Inflammation Triggers Zeb1-Dependent Escape from Tumor Latency
Authors: De Cock Jasmine M. , Shibue Tsukasa , Dongre Anushka , Keckesova Zuzana , Reinhardt Ferenc , Weinberg Robert A. 
Published: August 2016

Abstract
The emergence of metastatic disease in cancer patients many years or decades after initial successful treatment of primary tumors is well documented but poorly understood at the molecular level. Recent studies have begun exploring the cell-intrinsic programs, causing disseminated tumor cells to enter latency and the cellular signals in the surrounding nonpermissive tissue microenvironment that maintain the latent state. However, relatively little is known about the mechanisms that enable disseminated tumor cells to escape cancer dormancy or tumor latency. We describe here an in vivo model of solitary metastatic latency in the lung parenchyma. The induction of a localized inflammation in the lungs, initiated by lipopolysaccharide treatment, triggers the awakening of these cells, which develop into macroscopic metastases. The escape from latency is dependent on the expression of Zeb1, a key regulator of the epithelial-to-mesenchymal transition (EMT). Furthermore, activation of the EMT program on its own, as orchestrated by Zeb1, is sufficient to incite metastatic outgrowth by causing carcinoma cells to enter stably into a metastasis-initiating cell state. Cancer Res; 76(23); 6778–84. ©2016 AACR.
Reactive Oxygen Species Mediates the Synergistic Activity of Fenretinide Combined with the Microtubule Inhibitor ABT-751 against Multidrug-Resistant Recurrent Neuroblastoma Xenografts
Authors: Chen Nancy E. , Maldonado N. Vanessa , Khankaldyyan Vazgen , Shimada Hiroyuki , Song Michael M. , Maurer Barry J. , Reynolds C. Patrick 
Published: August 2016

Abstract
ABT-751 is a colchicine-binding site microtubule inhibitor. Fenretinide (4-HPR) is a synthetic retinoid. Both agents have shown activity against neuroblastoma in laboratory models and clinical trials. We investigated the antitumor activity of 4-HPR + the microtubule-targeting agents ABT-751, vincristine, paclitaxel, vinorelbine, or colchicine in laboratory models of recurrent neuroblastoma. Drug cytotoxicity was assessed in vitro by a fluorescence-based assay (DIMSCAN) and in subcutaneous xenografts in nu/nu mice. Reactive oxygen species levels (ROS), apoptosis, and mitochondrial depolarization were measured by flow cytometry; cytochrome c release and proapoptotic proteins were measured by immunoblotting. 4-HPR + ABT-751 showed modest additive or synergistic cytotoxicity, mitochondrial membrane depolarization, cytochrome c release, and caspase activation compared with single agents in vitro; synergism was inhibited by antioxidants (ascorbic acid, α-tocopherol). 4-HPR + ABT-751 was highly active against four xenograft models, achieving multiple maintained complete responses. The median event-free survival (days) for xenografts from 4 patients combined was control = 28, 4-HPR = 49, ABT-751 = 77, and 4-HPR + ABT-751 &gt; 150 (P &lt; 0.001). Apoptosis (terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling, TUNEL) was significantly higher in 4-HPR + ABT-751–treated tumors than with single agents (P &lt; 0.01) and was inhibited by ascorbic acid and α-tocopherol (P &lt; 0.01), indicating that ROS from 4-HPR enhanced the activity of ABT-751. 4-HPR also enhanced the activity against neuroblastoma xenografts of vincristine or paclitaxel, but the latter combinations were less active than 4-HPR + ABT-751. Our data support clinical evaluation of 4-HPR combined with ABT-751 in recurrent and refractory neuroblastoma. Mol Cancer Ther; 15(11); 2653–64. ©2016 AACR.
Dietary Methyl Donor Depletion Suppresses Intestinal Adenoma Development
Authors: Hanley Matthew P. , Kadaveru Krishna , Perret Christine , Giardina Charles , Rosenberg Daniel W. 
Published: August 2016

Abstract
The role of folate one-carbon metabolism in colorectal cancer development is controversial, with nutritional intervention studies producing conflicting results. It has been reported that ApcMin/+ mice maintained on a diet deficient in the methyl donors folic acid, methionine, choline, and vitamin B12, and supplemented with homocysteine, show a greater than 95% reduction in intestinal tumor development. The present study extends these findings and shows that tumor protection afforded by dietary methyl donor deficiency (MDD) is long-lasting. After 11 weeks of MDD, tumor protection persisted for at least an additional 7 weeks of methyl donor repletion (22.2 ± 3.5 vs. 70.2 ± 4.6 tumors per mouse; P &lt; 0.01). Sustained tumor protection was associated with a reduction in intestinal crypt length (26%, P &lt; 0.01), crypt cell division and crypt fission, and an increase in apoptosis of both normal crypts and tumors (4.9- and 3.2-fold, respectively, P &lt; 0.01). MDD also caused a significant reduction in the number of Dclk1-positive cells in the intestine (62%, P &lt; 0.01), a long-lived crypt cell with cancer stem cell potential. Several undesirable effects associated with methyl donor restriction (e.g., reduced body weight gain) were shown to be transient and readily reversible following methyl donor repletion. Taken together, these results indicate that even temporary dietary methyl donor restriction in adenoma-prone mice can induce persistent changes to the intestinal epithelium and provide long-lasting tumor protection. These data also suggest that transient reductions in dietary methyl donor consumption should be considered when studying the impact of folate on colon cancer risk in humans. Cancer Prev Res; 9(10); 812–20. ©2016 AACR.
Ensino de saúde do trabalhador nos cursos de graduação em Terapia Ocupacional: contribuições ao debate sobre parâmetros de avaliação do ensino público brasileiro
Authors: Pereira Jorge Iranise Moro , Simonelli Angela Paula , Rosa Jessica Elisa da , Ferreira Sattylla Sanny de Matos , Souza Marina Batista Chaves Azevedo de , Barroso Barbara Iansã De Lima 
Published: August 2016

Esta pesquisa possui como objetivo, investigar as ações de pesquisa, ensino e extensão desenvolvidas na área de Saúde do Trabalhador nos cursos de graduação em Terapia Ocupacional das universidades públicas do Brasil. Trata-se de pesquisa descritiva exploratória, realizada por duas Universidades Federais. A amostra foi composta por (n=16) cursos de graduação em Terapia Ocupacional de (n=15) universidades públicas. Os dados encontrados demonstram que as universidades abordam a área de Saúde do Trabalhador. Dispõem, em sua maioria, de professores com titulação na área de Ergonomia e apresentam, nos últimos três anos, (n=08) campos de estágio, (n=14) projetos de extensão, (n=70) trabalhos de conclusão de curso, (n=20) projetos de pesquisas e (n=29) publicações em periódicos científicos. Destaca-se a necessidade de ações que promovam a intersetorialização e o debate entre instituições, a fim de possibilitar troca de experiências e avanços no conteúdo do ensino,tanto neste campo de atuação, quanto na formação dos docentes responsáveis pela área de Saúde do Trabalhador.
Inclusão profissional com deficiência física em um serviço de saúde do Estado de Pernambuco
Authors: Cabral Ana Karina Pessoa da Silva , Pereira Priscylla Emanuella Cavalcanti , Caldas Ada Salvetti Cavalcanti 
Published: August 2016

No Brasil, estima-se que 45,6 milhões de pessoas têm algum tipo de deficiência. Porém, grande parte destas pessoas não tem acesso ao mercado trabalho ou, quando inseridas nos postos de trabalho, encontram dificuldades, por falta de preparo da empresa, baixa escolaridade e falta de capacitação profissional. O objetivo dessa pesquisa foi investigar a inclusão laboral de trabalhadores com deficiência física (TCDF) em um serviço de saúde no Estado de Pernambuco. Trata-se de um estudo transversal de abordagem qualitativa. Foram identificadas 4 (quatro) funções ocupadas pelos 9 (nove) TCDF, sendo elas: maqueiro, auxiliar de recepção e portaria, almoxarife de nutrição e técnico de enfermagem. Observou-se uma associação e restrição das tarefas com os tipos de deficiência, em todas as funções estudadas. Conclui-se que, apesar de complexo, o processo de inclusão deve considerar as habilidades reais das pessoas com deficiência, contribuindo com isto para uma sociedade mais equitativa e inclusiva.
Sintomas osteomusculares e desempenho ocupacional: um estudo entre músicos instrumentistas no contexto do Distrito Federal, Brasil.
Authors: De Carvalho Andréia Louise A. , Pontes Tatiana Barcelos , Massa Lilian Dias Bernardo , Rodrigues Daniela Da Silva , Almeida Pedro H. T. Q. de 
Published: August 2016

Objetivo: Determinar a prevalência de sintomatologia osteomuscular entre músicos de uma universidade federal, verificando seu impacto sobre a participação em atividades de vidadiária e o desempenho de atividades laborais. Método: 26 músicos foram avaliados por meio do Questionário de Disfunção do Ombro, Braço e Mão e Questionário Nórdico de Sintomas Osteomusculares. A relação entre sintomatologia musculoesquelética e desempenho ocupacional foi avaliada por meio dos testes de Mann-Whitney, Teste de Correlação de Spearman e Teste de Chi-Quadrado. Resultados: Foi observado predomínio de queixas osteomusculares nas regiões dos membros superiores e tronco, com baixa procura dos músicos por tratamentos de saúde. Observaram-se diferenças significativas no desempenho de atividades de vida diária e realização de atividades relacionadas à prática musical entre participantes que apresentavam queixas osteomusculares em membros superiores. Conclusão: O desempenho da atividade musical pode levar a processos de adoecimento, influenciando as atividades laborais, e outras áreas do desempenho ocupacional.Ações voltadas para a prevenção e tratamento de queixas osteomusculares são importantes para a elaboração de estratégias que visam preservar e promover a saúde desta população.
Síndrome de Burnout em professores universitários
Authors: Massa Lilian Dias Bernardo , Silva Talita Silvério de Souza , Sá Isabela Sebastian Vieira Barbosa , Barreto Bárbara Costa de Sá , Almeida Pedro Henrique Tavares Queiroz de , Pontes Tatiana Barcelos 
Published: August 2016

O estudo investigou os sinais indicativos da Síndrome de Burnout professores de ensino superior, bem como as estratégias de enfrentamento utilizadas para manter-se na atividade laboral. Participaram 49 professores. Foi aplicada a Escala de Caracterização de Burnout e um questionário para obtenção dos dados demográficos e identificação das estratégias de enfrentamento. Os dados foram analisados e apresentados em estatísticas descritas e a regressão logística foi usada para verificar a associação entre as variáveis demográficas e Burnout. Os resultados apontam que cerca de um quarto dos participantes apresentou sintomas compatíveis com a Síndrome de Burnout. A desumanização foi o item com maiorpercentual de alto nível, reportada por 30,6% dos professores. Tendo em vista o caráter multifatorial da síndrome de Burnout, é importante compreender os fatores laborais e psicossociais que podem estar associados ao adoecimento, pois isso pode contribuir para a inserção dos docentes em intervenções voltadas para o bem-estar e qualidade de vida no trabalho.
Programa de Atenção Integral à Saúde do Artista de Performance: relato da experiência desenvolvida em um serviço universitário em Minas Gerais
Authors: Lima Ronise Costa , Silva Talita Naiara Rossi da , Alves Gisele Beatriz de Oliveira , Fonseca João Gabriel Marques , Sampaio Rosana Ferreira , Lacerda Leonardo Lobão , Pinheiro Tarcísio Márcio Magalhães 
Published: August 2016

Trata-se de um relato de experiência cujo objetivo foi apresentar o Programa de Atenção Integral a Saúde do Artista de Performance do Serviço Especializado em Saúde do Trabalhador do Hospital das Clínicas da Universidade Federal de Minas Gerais e relatar as ações desenvolvidas pela equipe interdisciplinar. As ações compreendem avaliação clínico-ocupacional, incluindo análise da atividade com o instrumento musical; visitas técnicas aoslocais de trabalho, assistência individual ou atividades em grupo e, ações educativas e preventivas, como a elaboração de um folheto informativo e workshops sobre a saúde do músico. De dezembro de 2009 a maio de 2015 foram atendidos 122 músicos, sendo amaioria instrumentistas, do sexo masculino, com média de idade de 32 anos. As reavaliações e os relatos dos trabalhadores assistidos demonstraram que os objetivos do programa vêm sendo alcançados, principalmente no que diz respeito ao autogerenciamento das estratégias de enfrentamento dos riscos de adoecimento no trabalho. Para dar sustentação científica aos resultados, foi elaborado um protocolo sistematizando as atividades em grupo, que se encontra em fase de avaliação. Espera-se que a experiência do SEST com relação a saúde do músico possa auxiliar outros profissionais e serviços a se organizar frente as demandas deste coletivo.
Predictors of Physician Under-Recognition of Angina in Outpatients With Stable Coronary Artery Disease
Authors: Arnold Suzanne V. , Grodzinsky Anna , Gosch Kensey L. , Kosiborod Mikhail , Jones Philip G. , Breeding Tracie , Towheed Arooge , Beltrame John , Alexander Karen P. , Spertus John A. 
Published: August 2016

Background—
Under-recognition of angina by physicians may result in undertreatment with revascularization or medications that could improve patients’ quality of life. We sought to describe characteristics associated with under-recognition of patients’ angina.

Methods and Results—
Patients with coronary disease from 25 US cardiology outpatient practices completed the Seattle Angina Questionnaire before their clinic visit, quantifying their frequency of angina during the previous month. Immediately after the clinic visit, physicians independently quantified their patients’ angina. Angina frequency was categorized as none, monthly, and daily/weekly. Among 1257 patients, 411 reported angina in the previous month, of whom 173 (42%) were under-recognized by their physician, defined as the physician reporting a lower frequency category of angina than the patient. In a hierarchical logistic model, heart failure (odds ratio, 3.06, 95% confidence interval, 1.89–4.95) and less-frequent angina (odds ratio for monthly angina [versus daily/weekly], 1.69; 95% confidence interval, 1.12–2.56) were associated with greater odds of under-recognition. No other patient or physician factors were associated with under-recognition. Significant variability across physicians (median odds ratio, 2.06) was observed.

Conclusions—
Under-recognition of angina is common in routine clinical practice. Although patients with less-frequent angina and those with heart failure more often had their angina under-recognized, most variation was unrelated to patient and physician characteristics. The large variation across physicians suggests that some physicians are more accurate in assessing angina frequency than others. Standardized prospective use of a validated clinical tool, such as the Seattle Angina Questionnaire, should be tested as a means to improve recognition of angina and, potentially, improve appropriate treatment of angina.
Race‐Specific Predictors of Mortality in Intracerebral Hemorrhage: Differential Impacts of Intraventricular Hemorrhage and Age Among Blacks and Whites
Authors: Faigle Roland , Marsh Elisabeth B. , Llinas Rafael H. , Urrutia Victor C. , Gottesman Rebecca F. 
Published: August 2016

Background

Intracerebral hemorrhage (
ICH
) carries high risk for short‐term mortality. We sought to identify race‐specific predictors of mortality in
ICH
patients.

Methods and Results

We used 2 databases, the Johns Hopkins clinical stroke database and the Nationwide Inpatient Sample (
NIS
). We included 226 patients with the primary diagnosis of spontaneous
ICH
from our stroke database between 2010 and 2013; in the
NIS
, 42 077 patients met inclusion criteria. Logistic regression was used to assess differences in predictors of mortality in blacks compared to whites. In our clinical stroke database, Glasgow Coma Scale (
GCS
;
P
=0.016),
ICH
volume (
P
=0.013), intraventricular haemorrhage (
IVH
;
P
=0.023), and diabetes mellitus (
P
=0.037) were predictors of mortality in blacks, whereas
GCS
(
P
=0.007),
ICH
volume (
P
=0.005), age (
P
=0.002), chronic kidney disease (
P
=0.003), and smoking (
P
=0.010) predicted mortality in whites. Among patients with
IVH
, blacks had over 7 times higher odds of mortality compared to whites (odds ratio [
OR
], 7.27;
P
value for interaction, 0.017) and were more likely to present with hydrocephalus (
OR
, 2.76;
P
=0.026). In the
NIS
, black
ICH
patients had higher rates of external ventricular drain (
EVD
) placement compared to whites (9.7% vs 5.0%;
P
<0.001) and were more likely to develop hydrocephalus (
OR
, 1.32; 95%
CI
, 1.20–1.46). Comparison of a race‐specific
ICH
score to the original
ICH
score showed that the various
ICH
score components have differential relevance for
ICH
score performance by race.

Conclusions

IVH
and age differentially predict mortality among blacks and whites. Blacks have higher rates of obstructive hydrocephalus and more frequently require
EVD
placement compared to their white counterparts.
The effects of a home-based arm ergometry exercise programme on physical fitness, fatigue and activity in Polio survivors: a randomised controlled trial
Authors: Murray D , Hardiman O , Campion A , Vance R , Horgan F , Meldrum D 
Published: August 2016

Objective:To investigate the effect of an eight-week home-based arm ergometry aerobic exercise programme on physical fitness, fatigue, activity and quality of life in Polio Survivors.Design:An assessor blinded randomised controlled trial.Setting:Home-based exercise.Subjects:Fifty-five Polio survivors randomised to exercise or control groups.Intervention:Home-based arm ergometry at an intensity of 50%-70% maximum heart rate, compared with usual physiotherapy care.Main measures:The Six-minute Arm Test, Fatigue Severity Scale, Physical Activity Scale for Individuals with Physical Disabilities and SF-36. Assessments were completed at baseline and at eight weeks.Results:There was no significant difference in the primary outcome, exercising heart rate during the Six-minute Arm Test, between the groups at follow-up [97.6 (SD10.1) compared to 102.4 (SD13.7) beats per minute ( P=0.20)]. Blood pressure was significantly lower in the intervention group at follow-up [systolic blood pressure 132(18.6)mmHg compared to 144.1(14.6)mmHg ( P=0.002)]. There were no between group differences in the Fatigue Severity Scale ( P=0.25) or Physical Activity Scale for Individuals with Physical Disabilities ( P=0.49), with a small difference in SF-36 physical component score ( P=0.04).Conclusions:This home-based arm ergometry programme successfully facilitated aerobic exercise in Polio Survivors, but did not result in a significant change in physical fitness, measured by the Six-minute Arm Test.