bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput fluorescence lifetime imaging flow cytometry
Authors: Kanno Hiroshi , Hiramatsu Kotaro , Mikami Hideharu , Nakayashiki Atsushi , Yamashita Shota , Nagai Arata , Okabe Kohki , Li Fan , Yin Fei , Tominaga Keita , Bicer Omer Faruk , Noma Ryohei , Kiani Bahareh , Efa Olga , Büscher Martin , Wazawa Tetsuichi , Sonoshita Masahiro , Shintaku Hirofumi , Nagai Takeharu , Braun Sigurd , Houston Jessica P. , Rashad Sherif , Niizuma Kuniyasu , Goda Keisuke 
Published: September 2024

AbstractFlow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the full integration of FLIM into flow cytometry has yet to be demonstrated due to speed limitations. Here we overcome the speed limitations in FLIM, thereby enabling high-throughput FLIM flow cytometry at a high rate of over 10,000 cells per second. This is made possible by using dual intensity-modulated continuous-wave beam arrays with complementary modulation frequency pairs for fluorophore excitation and acquiring fluorescence lifetime images of rapidly flowing cells. Moreover, our FLIM system distinguishes subpopulations in male rat glioma and captures dynamic changes in the cell nucleus induced by an anti-cancer drug. FLIM flow cytometry significantly enhances cellular analysis capabilities, providing detailed insights into cellular functions, interactions, and environments.
Photosynthetic light requirement near the theoretical minimum detected in Arctic microalgae
Authors: Hoppe Clara J. M. , Fuchs Niels , Notz Dirk , Anderson Philip , Assmy Philipp , Berge Jørgen , Bratbak Gunnar , Guillou Gaël , Kraberg Alexandra , Larsen Aud , Lebreton Benoit , Leu Eva , Lucassen Magnus , Müller Oliver , Oziel Laurent , Rost Björn , Schartmüller Bernhard , Torstensson Anders , Wloka Jonas 
Published: September 2024

AbstractPhotosynthesis is one of the most important biological processes on Earth, providing the main source of bioavailable energy, carbon, and oxygen via the use of sunlight. Despite this importance, the minimum light level sustaining photosynthesis and net growth of primary producers in the global ocean is still unknown. Here, we present measurements from the MOSAiC field campaign in the central Arctic Ocean that reveal the resumption of photosynthetic growth and algal biomass buildup under the ice pack at a daily average irradiance of not more than 0.04 ± 0.02 µmol photons m−2 s−1 in late March. This is at least one order of magnitude lower than previous estimates (0.3–5 µmol photons m−2 s−1) and near the theoretical minimum light requirement of photosynthesis (0.01 µmol photons m−2 s−1). Our findings are based on measurements of the temporal development of the under-ice light field and concurrent measurements of both chlorophyll a concentrations and potential net primary production underneath the sea ice at 86 °N. Such low light requirements suggest that euphotic zones where photosynthesis can occur in the world’s oceans may extend further in depth and time, with major implications for global productivity estimates.
Hydrogen storage with gravel and pipes in lakes and reservoirs
Authors: Hunt Julian David , Nascimento Andreas , Romero Oldrich Joel , Zakeri Behnam , Jurasz Jakub , Dąbek Paweł B. , Strzyżewski Tomasz , Đurin Bojan , Leal Filho Walter , Freitas Marcos Aurélio Vasconcelos , Wada Yoshihide 
Published: September 2024

AbstractClimate change is projected to have substantial economic, social, and environmental impacts worldwide. Currently, the leading solutions for hydrogen storage are in salt caverns, and depleted natural gas reservoirs. However, the required geological formations are limited to certain regions. To increase alternatives for hydrogen storage, this paper proposes storing hydrogen in pipes filled with gravel in lakes, hydropower, and pumped hydro storage reservoirs. Hydrogen is insoluble in water, non-toxic, and does not threaten aquatic life. Results show the levelized cost of hydrogen storage to be 0.17 USD kg−1 at 200 m depth, which is competitive with other large scale hydrogen storage options. Storing hydrogen in lakes, hydropower, and pumped hydro storage reservoirs increases the alternatives for storing hydrogen and might support the development of a hydrogen economy in the future. The global potential for hydrogen storage in reservoirs and lakes is 3 and 12 PWh, respectively. Hydrogen storage in lakes and reservoirs can support the development of a hydrogen economy in the future by providing abundant and cheap hydrogen storage.
Control limitations in the null-space of the wrist muscle system
Authors: Lee Meng-Jung , Eden Jonathan , Gurgone Sergio , Berger Denise J , Borzelli Daniele , d’Avella Andrea , Mehring Carsten , Burdet Etienne 
Published: September 2024

AbstractThe redundancy present within the musculoskeletal system may offer a non-invasive source of signals for movement augmentation, where the set of muscle activations that do not produce force/torque (muscle-to-force null-space) could be controlled simultaneously to the natural limbs. Here, we investigated the viability of extracting movement augmentation control signals from the muscles of the wrist complex. Our study assessed (i) if controlled variation of the muscle activation patterns in the wrist joint’s null-space is possible; and (ii) whether force and null-space cursor targets could be reached concurrently. During the null-space target reaching condition, participants used muscle-to-force null-space muscle activation to move their cursor towards a displayed target while minimising the exerted force as visualised through the cursor’s size. Initial targets were positioned to require natural co-contraction in the null-space and if participants showed a consistent ability to reach for their current target, they would rotate 5$$^\circ$$

∘

incrementally to generate muscle activation patterns further away from their natural co-contraction. In contrast, during the concurrent target reaching condition participants were required to match a target position and size, where their cursor position was instead controlled by their exerted flexion–extension and radial-ulnar deviation, while its size was changed by their natural co-contraction magnitude. The results collected from 10 participants suggest that while there was variation in each participant’s co-contraction behaviour, most did not possess the ability to control this variation for muscle-to-force null-space virtual reaching. In contrast, participants did show a direction and target size dependent ability to vary isometric force and co-contraction activity concurrently. Our results indicate the limitations of using the muscle-to-force null-space activity of joints with a low level of redundancy as a possible command signal for movement augmentation.
Functional outcomes and quality of life after AcrySof IQ Vivity intraocular lens implantation in a real-world study
Authors: Giannuzzi Federico , Carlà Matteo Mario , Margollicci Fabio , Di Stefano Gaetano , Molle Andrea , Hu Lorenzo , Boselli Francesco , Pagliara Monica Maria , Lepore Domenico , Molle Fernando , Rizzo Stanislao 
Published: September 2024

AbstractThe extended depth-of-focus AcrySof IQ Vivity intraocular lens technology offers promising features for presbyopia management, evaluated in this research in a 6 months real-world setting. Prospective interventional mono-centric study including 40 patients who underwent elective bilateral phacoemulsification. We performed one pre-operative visit (V0) and one evaluation six months post-operatively (V1), evaluating uncorrected and corrected visual acuity for near (UNVA/CNVA), intermediate (UIVA/CIVA) and far (UDVA/UCVA), slit-lamp evaluation, tomography with static pupillometry, endothelial cell count and contrast sensitivity chart. In order to assess post-operative Quality of Life, we administered the patients McAlinden’s Quality of Vision test and Morlock’s Patient-Reported Spectacle Independence Questionnaire. We divided eyes in with Toric-IOL and with non-Toric IOL. A total of 36 eyes received non-tonic IOL implantation, whereas 44 eyes received toric IOL implantation. There were no statistically significant disparities observed in visual outcome measures and contrast sensitivity between the toric group and the non-toric group. Furthermore, we assessed the predictive preoperative refractive astigmatism (PPRA) and residual refractive astigmatism (RRA) in both cohorts, and no statistical significance was found between the two cohorts (p = 0.08). Twenty-one (53%) patients reported total independence from their glasses at all distances. The mean difference between the predicted and measured refractive error, as calculated by spherical equivalent, was 0.09 D. AcrySof IQ Vivity is a well-tolerated and effective IOL with optimal refractive target for both distant and intermediate vision, needing slight spherical addition for the best near vision. Great questionnaire-based satisfaction was reported by the patients.
Sensing the structural and conformational properties of single-stranded nucleic acids using electrometry and molecular simulations
Authors: Walker-Gibbons Rowan , Zhu Xin , Behjatian Ali , Bennett Timothy J. D. , Krishnan Madhavi 
Published: September 2024

AbstractInferring the 3D structure and conformation of disordered biomolecules, e.g., single stranded nucleic acids (ssNAs), remains challenging due to their conformational heterogeneity in solution. Here, we use escape-time electrometry (ETe) to measure with sub elementary-charge precision the effective electrical charge in solution of short to medium chain length ssNAs in the range of 5–60 bases. We compare measurements of molecular effective charge with theoretically calculated values for simulated molecular conformations obtained from Molecular Dynamics simulations using a variety of forcefield descriptions. We demonstrate that the measured effective charge captures subtle differences in molecular structure in various nucleic acid homopolymers of identical length, and also that the experimental measurements can find agreement with computed values derived from coarse-grained molecular structure descriptions such as oxDNA, as well next generation ssNA force fields. We further show that comparing the measured effective charge with calculations for a rigid, charged rod—the simplest model of a nucleic acid—yields estimates of molecular structural dimensions such as linear charge spacings that capture molecular structural trends observed using high resolution structural analysis methods such as X-ray scattering. By sensitively probing the effective charge of a molecule, electrometry provides a powerful dimension supporting inferences of molecular structural and conformational properties, as well as the validation of biomolecular structural models. The overall approach holds promise for a high throughput, microscopy-based biomolecular analytical approach offering rapid screening and inference of molecular 3D conformation, and operating at the single molecule level in solution.
Semaphorin 3F (SEMA3F) influences patient survival in esophageal adenocarcinoma
Authors: Knipper Karl , Lyu Su Ir , Jung Jin-On , Alich Niklas , Popp Felix C. , Schröder Wolfgang , Fuchs Hans F. , Bruns Christiane J. , Quaas Alexander , Nienhueser Henrik , Schmidt Thomas 
Published: September 2024

AbstractIn esophageal adenocarcinoma, the presence of lymph node metastases predicts patients' survival even after curative resection. Currently, there is no highly accurate marker for detecting the presence of lymph node metastasis. The SEMA3F/NRP2 axis was initially characterized in axon guidance and recent evidence has revealed its significant involvement in lymphangiogenesis, angiogenesis, and carcinogenesis. Hence, the objective of this study was to elucidate the roles of SEMA3F and its receptor NRP2 in esophageal adenocarcinoma. We conducted an immunohistochemical evaluation of SEMA3F and NRP2 protein expression in 776 patients with esophageal adenocarcinoma who underwent Ivor-Lewis esophagectomy at the University Hospital of Cologne. Total and positive cancer cell counts were digitally analyzed using QuPath and verified by experienced pathologists to ensure accuracy. Positive expression was determined as a cell percentage exceeding the 50th percentile threshold. In our cohort, patients exhibiting SEMA3F positive expression experience significantly lower pT- and pN-stages. In contrast, positive NRP2 expression is associated with the presence of lymph node metastases. Survival analyses showed that the expression status of NRP2 had no impact on patient survival. However, SEMA3F positivity was associated with a favorable patient survival outcome (median OS: 38.9 vs. 26.5 months). Furthermore, SEMA3F could be confirmed as an independent factor for better patient survival in patients with early tumor stage (pT1N0-3: HR = 0.505, p = 0.014, pT1-4N0: HR = 0.664, p = 0.024, pT1N0: HR = 0.483, p = 0.040). In summary, SEMA3F emerges as an independent predictor for a favorable prognosis in patients with early-stage esophageal adenocarcinoma. Additionally, NRP2 expression is linked to a higher risk of lymph node metastases occurrence. We hypothesize that low SEMA3F expression could identify patients with early-stage tumors who might benefit from more aggressive treatment options or intensified follow-up. Furthermore, SEMA3F and its associated pathways should be explored as potential tumor-suppressing agents.
Satellitome analysis on the pale-breasted thrush Turdus leucomelas (Passeriformes; Turdidae) uncovers the putative co-evolution of sex chromosomes and satellite DNAs
Authors: Souza Guilherme Mota , Kretschmer Rafael , Toma Gustavo Akira , de Oliveira Alan Moura , Deon Geize Aparecida , Setti Princia Grejo , Zeni dos Santos Rodrigo , Goes Caio Augusto Gomes , Del Valle Garnero Analía , Gunski Ricardo José , de Oliveira Edivaldo Herculano Correa , Porto-Foresti Fabio , Liehr Thomas , Utsunomia Ricardo , de Bello Cioffi Marcelo 
Published: September 2024

AbstractDo all birds' sex chromosomes follow the same canonical one-way direction of evolution? We combined cytogenetic and genomic approaches to analyze the process of the W chromosomal differentiation in two selected Passeriform species, named the Pale-breasted Thrush Turdus leucomelas and the Rufous-bellied thrush T. rufiventris. We characterized the full catalog of satellite DNAs (satellitome) of T. leucomelas, and the 10 TleSatDNA classes obtained together with 16 microsatellite motifs were in situ mapped in both species. Additionally, using Comparative Genomic Hybridization (CGH) assays, we investigated their intragenomic variations. The W chromosomes of both species did not accumulate higher amounts of both heterochromatin and repetitive sequences. However, while T. leucomelas showed a heterochromatin-poor W chromosome with a very complex evolutionary history, T. rufiventris showed a small and partially heterochromatic W chromosome that represents a differentiated version of its original autosomal complement (Z chromosome). The combined approach of CGH and sequential satDNA mapping suggest the occurrence of a former W-autosomal translocation event in T. leucomelas, which had an impact on the W chromosome in terms of sequence gains and losses. At the same time, an autosome, which is present in both males and females in a polymorphic state, lost sequences and integrated previously W-specific ones. This putative W-autosomal translocation, however, did not result in the emergence of a multiple-sex chromosome system. Instead, the generation of a neo-W chromosome suggests an unexpected evolutionary trajectory that deviates from the standard canonical model of sex chromosome evolution.
Elastin-specific MR probe for visualization and evaluation of an interleukin-1β targeted therapy for atherosclerosis
Authors: Mangarova Dilyana Branimirova , Reimann Carolin , Kaufmann Jan Ole , Möckel Jana , Kader Avan , Adams Lisa Christine , Ludwig Antje , Onthank David , Robinson Simon , Karst Uwe , Helmer Rebecca , Botnar Rene , Hamm Bernd , Makowski Marcus Richard , Brangsch Julia 
Published: September 2024

AbstractAtherosclerosis is a chronic inflammatory condition of the arteries and represents the primary cause of various cardiovascular diseases. Despite ongoing progress, finding effective anti-inflammatory therapeutic strategies for atherosclerosis remains a challenge. Here, we assessed the potential of molecular magnetic resonance imaging (MRI) to visualize the effects of 01BSUR, an anti-interleukin-1β monoclonal antibody, for treating atherosclerosis in a murine model. Male apolipoprotein E-deficient mice were divided into a therapy group (01BSUR, 2 × 0.3 mg/kg subcutaneously, n = 10) and control group (no treatment, n = 10) and received a high-fat diet for eight weeks. The plaque burden was assessed using an elastin-targeted gadolinium-based contrast probe (0.2 mmol/kg intravenously) on a 3 T MRI scanner. T1-weighted imaging showed a significantly lower contrast-to-noise (CNR) ratio in the 01BSUR group (pre: 3.93042664; post: 8.4007067) compared to the control group (pre: 3.70679168; post: 13.2982156) following administration of the elastin-specific MRI probe (p < 0.05). Histological examinations demonstrated a significant reduction in plaque size (p < 0.05) and a significant decrease in plaque elastin content (p < 0.05) in the treatment group compared to control animals. This study demonstrated that 01BSUR hinders the progression of atherosclerosis in a mouse model. Using an elastin-targeted MRI probe, we could quantify these therapeutic effects in MRI.
Structure of tetrameric forms of the serotonin-gated 5-HT3A receptor ion channel
Authors: Introini Bianca , Cui Wenqiang , Chu Xiaofeng , Zhang Yingyi , Alves Ana Catarina , Eckhardt-Strelau Luise , Golusik Sabrina , Tol Menno , Vogel Horst , Yuan Shuguang , Kudryashev Mikhail 
Published: September 2024

AbstractMultimeric membrane proteins are produced in the endoplasmic reticulum and transported to their target membranes which, for ion channels, is typically the plasma membrane. Despite the availability of many fully assembled channel structures, our understanding of assembly intermediates, multimer assembly mechanisms, and potential functions of non-standard assemblies is limited. We demonstrate that the pentameric ligand-gated serotonin 5-HT3A receptor (5-HT3AR) can assemble to tetrameric forms and report the structures of the tetramers in plasma membranes of cell-derived microvesicles and in membrane memetics using cryo-electron microscopy and tomography. The tetrameric structures have near-symmetric transmembrane domains, and asymmetric extracellular domains, and can bind serotonin molecules. Computer simulations, based on our cryo-EM structures, were used to decipher the assembly pathway of pentameric 5-HT3R and suggest a potential functional role for the tetrameric receptors.
Gas flows in the central region of the Seyfert galaxy NGC 4593 with MUSE
Authors: Mulumba D. , Knapen J. H. , Comerón S. , Ramos Almeida C. , Obonyo W. O. , Pereira-Santaella M. , Baki P. 
Published: September 2024

Context. Understanding how gas flows into galactic centres, fuels the active galactic nucleus (AGN), and is in turn expelled back through feedback processes is of great importance to appreciate the role AGN play in the growth and evolution of galaxies.
Aims. We use Multi Unit Spectroscopic Explorer-adaptive optics (MUSE-AO) optical spectra of the inner 7″​.5 × 7″​.5 (1.3 kpc × 1.3 kpc) of the nearby Seyfert 1 galaxy NGC 4593 to characterise its ionised gas kinematics.
Methods. We fitted single-Gaussian components to the [O III] λ5007 and [N II] λ6583 emission lines, and double-Gaussian components to Hα and Hβ to determine the main ionisation mechanism of the gas. To determine the kinematics of the ionised gas, we fit double-Gaussian components to the [O III] λ5007 line.
Results. The high angular resolution MUSE data (∼0″​​​​. 12 = 20 pc) capture structures of the circumnuclear region including the innermost spiral that feeds the nucleus. Based on the stellar kinematic maps, we confirm the presence of a rotating disc, whilst for the ionised gas, we find high-velocity dispersion values of up to 200 − 250 km s−1 that show that part of the gas is highly perturbed. The dominant ionisation mechanism of the gas is AGN photoionisation, which reaches the highest values within the innermost 4″ (680 pc) diameter of the galaxy. At larger radii, the emission line ratios correspond to values in the composite region of the Baldwin, Phillips and Terlevich (BPT) diagram.
Conclusions. The broad-component of [O III] λ5007 shows blue-shifted velocities on the east side of the central 2″ (340 pc), which spatially coincide with a region of high velocity-dispersion. This confirms the presence of outflowing gas. We estimate a mass outflow rate and kinetic power of Ṁ ≥ 0.048 M⊙ yr−1 and Ėkin ≥ 4.09 × 1039 erg s−1. The derived mass outflow rate is consistent with that expected from empirical relations between mass outflow rate and AGN luminosity for a low-luminosity AGN such as NGC 4593. High angular resolution integral field observations can enable multi-component analysis of the innermost regions of galaxies, allowing a detailed view of ionised gas flows.
Tratamento conservador da camptodactilia com órteses: Coorte retrospectiva
Authors: Oliveira Maria da Conceição Soares de , Figueiredo Felipe Soares , Aguiar Diego Pinheiro 
Published: September 2024

Resumo
Objetivo Avaliar os resultados do tratamento conservador com o uso de órteses estáticas confeccionadas pelo Setor de Terapia Ocupacional da nossa instituição em participantes com camptodactilia dos tipos I, II e III, em suas formas rígida ou flexível, descrever os dados demográficos e clínicos, e avaliar o número de abandonos no período do tratamento.
Métodos O projeto foi aprovado pelo Comitê de Ética em Pesquisa em Seres Humanos, com número de protocolo CAAE 20300419.3.0000.5273. Todos os prontuários utilizados na pesquisa foram disponibilizados pela nossa instituição. Não foi feito o uso de termo de consentimento livre e esclarecido (TCLE) para este estudo retrospectivo devido à impossibilidade de contactar o elevado número de participantes. Foram incluídos os prontuários de 38 participantes atendidos no Ambulatório de Terapia Ocupacional de 2013 a 2019.
Resultados Dos 54 dedos tratados com órteses, 38 foram totalmente corrigidos. As taxas de correção foram as seguintes: tipo I na forma rígida –100%; tipo I na forma flexível –81,2%; tipo II na forma rígida – 0%; tipo II na forma flexível – 100%; tipo III na forma rígida –47,6%; e tipo III na forma flexível –100%. Do total de 93 dedos incluídos, 42% abandonaram o tratamento.
Conclusão O uso de órteses estáticas é uma alternativa segura ao procedimento cirúrgico e de baixa complexidade de execução para o tratamento da camptodactilia.