bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimation of genetic diversity of Karelian birch populations in Kareliausing microsatellite markers
Authors: Vetchinnikova Lidia V , Titov Alexander F , Topchieva Ludmila V , Rendakov Nikolay L 
Published: September 2017

We studied genetic structure of populations of Karelian birch Betula pendula var. carelica (Mercklin) Hämet-Ahti, whichlacks second growth. Based on the values of expected and observed heterozygosity, the predominantselection of homozygotes on the background of decrease of the heterozygotes fraction was revealed. Interpopulation differentiation rate (Fst = 0,145) indicates a high inbreeding frequency. Observed genetic characteristics of the populationssuggest that genetic mechanisms play a role in the current decrease of thepopulation size of Karelian birch and its genetic degradation.
Genetic study of motor functions in Drosophila melanogaster
Authors: Fedotov Sergey A , Bragina Julia V , Besedina Nataliya G , Danilenkova Larisa V , Kamysheva Elena A , Kamyshev Nikolay G 
Published: September 2017

To investigate molecular mechanisms of central pattern generators (CPG s) functioning, we carried out a screening of collection of Drosophila P-insertional mutants for strong deviations in locomotion and courtship song. In 21 mutants, the site of the P-insertion was localized by sequencing of the fragments of genomic DNA flanking the P-element. Bioinformational analysis revealed a list of candidate genes, potential players in development and functioning of CPG s. Possible involvement of certain identified genes in rhythmic motor activity is suggested for the first time (CG15630, Map205).
GENETIC ESTIMATION OF SNAIL LYMNAEA STAGNALIS POPULATIONS FROM REGIONS WITH DIFFERENT ANTHROPOGENIC LOAD AS A FIRST STEP IN GENETIC MONITORING
Authors: Koneva Oksana Yu 
Published: September 2017

The paper deals with carrying out molecular-genetic typing of populations of snail Lymnaea stagnalis from regions with a different ecological load (the lake Perstok and zatoka of the river Pripyat, Belarus). By means of interpopulation analysis of frequencies of RAPD-fragments, frequencies of zero alleles of RAPD-loci and genetic similarity index we established that these populations are genetically identical because there are no significant distinctions of genetic variability neither between individuals within a population, nor between populations. Results of the cluster analysis and the population analysis also have confirmed that populations of snails are genetically closely related. However the snail population of the lake Perstok is genetically more homogeneous.
The role of histone modifications and DNA methylation in renal cell carcinoma development
Authors: Kutlyeva Lilia R , Gilayzova Irina R , Khusainova Rita I , Khusnutdinova Elsa K 
Published: September 2017

Epigenetic mechanisms of gene regulation play a key role in carcinogenesis. This review will focus on the recent advances of epigenetic investigations in the development of human cancer. The role of histone modifications, genomic imprinting and DNA methylation in renal cell carcinoma development and progression will be considered.
Characteristics of morphogenetic responses of Nicotiana langsdorffii on transformation with wild type strains of Agrobacterium in vitro
Authors: Matveeva Tatyana V , Pigichka Tatiana Yu , Lutova Ludmila A 
Published: September 2017

Ability to transformation by wt strains of Agrobacterium tumefaciens (T37, C58, A6) and A. rhizogenes (15834, 8196, A4) was characterized for Nicotiana langsdorffii. It was shown that effectivity of transformation of this species by strains T37, A6,15834, 8196, A4 was lower comparing to the model object N. tabacum. Tumors induced by A. tumefaciens on leaf disks of N. langsdorffii, tend to shoot formation.
Molecular genetic analysis of Arabidopsis thaliana cold sensitive mutant nfz24
Authors: Novokreschenova Maria G , Soldatova Olga P , Volkova Ludmila A , Burgutin Aleksander B , Ezhova Tatyana A 
Published: September 2017

Development of stress-resistant plant varieties is a main task of plant biotechnology which is gaining popularity nowadays following the climatic changes and increased environmental pollution. This task implementation is primarily depends on identification of new genes responsible for stress resistance and also on the analysis of all functional features of genes on a cellular, tissue and organ levels. This article is dedicated to the study of Arabidopsis thaliana mutant nfz24 and the analysis of theNFZ24 gene function in cold stress response. It was demonstrated that reduced carotinoid content and alteration in ABA dependent cold response are the reasons of nfz24 cold sensitivity.
Effects of peptides on antioxidant status and survival curve parameters in inbred pre-selected lines in Drosophila melanogaster
Authors: Mylnikov Sergey V , Oparina Tatyana I , Malinin Vladimir V 
Published: September 2017

We performed comparative genetic analysis of a set of synthetic peptides effects on longevity and free-radical oxidation in Drosophila melanogaster. Strong genotype and gene-environmental interaction in determining traits value were evaluated. Mechanisms of vilon and epithalon effect on free oxygen species in mitochondria seemed to be different.
BENEFICIAL RHIZOSPHERE PSEUDOMONADS
Authors: Lugtenberg Ben , Kamilova Faina D 
Published: September 2017

Among the many bacteria present on and around the root, Pseudomonas bacteria are (among) the best root colonizers and therefore very suitable to apply for beneficial purposes. In this chapter, we discuss the possibilities to use such bacteria for the following purposes: fertilization of the plant, stimulation of plant growth and yield, reduction of plant stress, and reduction of plant diseases. This research was supported by numerous grants, especially from the Dutch Organization for scientific research (NWO), EET, the European Commission and INTAS.
Creation of mutant collections for the study of genetic control of stress adaptation in Synechocystis sp
Authors: Zinchenko Vladislav V , Glazer Vadim M , Kryazhov Sergey V , Luchkin Pavel V , Babykin Mikhail M , Belavina Nataliya V , Los Dmitry A 
Published: September 2017

The availability of the complete genome sequence of cyanobacterium Synechocystis sp. PCC6803 which has been used as a model organism for molecular-genetic studying oxygenic photosynthesis and cell adaptation to stresses gives the opportunity to investigate the function of each gene in this organism by the targeted inactivation of the predicted genes. This paper presents our main results on functional genomics of Synechocystis based on construction and study of mutant collections with insertion inactivation of the genes encoding peptidases, translocases and the proteins of two-component regulatory systems.
Influence of mutations in regulatory PHO genes on stability of a genetic material of yeast Saccharomyces cerevisiae
Authors: Smirnov Arseny M , Sambuk Elena V 
Published: September 2017

Yeast Saccharomyces cerevisiae is convenient modelling object for studying of spontaneous mutations frequency under the influence of various environmental factors, and also as a result of metabolism infringement. One of necessary components of the growing media is inorganic phosphate. Its lack influences an expression of many genes. The system of genes expression regulation by phosphate is studied in detail. In the present work dependence of stability of a genetic material of a cage on its metabolic condition caused by mutations in genes, coding phosphate metabolism regulating proteins, is shown.
DEVELOPMENT OF THE DUCKWEED (LEMNA MINOR) GENETIC TRANSFORMATION SYSTEM
Authors: Gaydukova Sofia E , Rakitin Andrey L , Ravin Nikolay V , Skryabin Konstantin G , Kamionskaya Anastasia M 
Published: September 2017

Regeneration parameters were optimized and effective selection conditions were sorted out. Transgenic plants of Lemna minor were obtained and their status was confirmed by molecular analysis. Optimized methodology can be used for obtaining transgenic duckweed plants producing recombinant proteins and water body bioremediators.
Cytoplasmic incompatability in Drosophila melanogaster due to different Wolbachia genotypes
Authors: Ilinsky Yury Y , Zakharov Ilya K 
Published: September 2017

Wolbachia is an endosymbiont prevalent among different arthropoda and in some Nematoda species. The bacteria ads across the populations of host species via induction of reproductive abnormalities: cytoplasmic incompatability, parthenogenesis, feminization, and male killing. we estimated the level of cytoplasmic incompatability in Drosophila melanogaster caused by three most prevalent Wolbachia genotypes, namely wmel, wmelCS и wmelCS2. wmel and wmelCS genotypes were shown to cause mild cytoplasmic incompatibility ( 10 %), while wmelCS Wolbachia were unable to do so. Possible mechanisms of sustainability of Wolbachia in the populations of Drosophila melanogaster are discussed.table border="0" cellpadding="3" cellspacing="0"	tbody	/tbody/table
The association gene polymorphisms with risk of cancer in long-term «low-dose» y-irradiation exposure
Authors: Litvjakov Nikolay V , Freydin Maksim B , Takhauov Ravil M , Ageeva Alena M , Volkova Natalia M , Ivanina Polina V , Goncharik Olesya O , Vasilyeva Elena O , Skobelskaya Elena V , Karpov Andrey B 
Published: September 2017

The association of 13 biallelic polymorphisms of genes hOGG1 977C G, XPD1 2251A C, XPG1 3310G C, XRCC1:580C T, 839G A, 1196GA GSTT1 («+»/«-»), GSTM1 («+»/«-»), CYP2C19 681G A, NOS3: -665CT, 774С T, 894G T, VNTRint4 with risk of development of malignant new growths (MNG) in conditions of "low-dose" ionizing irradiation was investigated. In 1 group of irradiated patients of malignant new growths there are 96 persons, in 2 group of non-irradiated patients of MNG - 135 persons, in 3 control group - 148 healthy irradiated workers of the Siberian Group of Chemical Enterprises. By means of procedure FDR it is shown that only polymorphic variants of gene CYP2C19681G* are authentically associated with risk of occurrence of MNG against an irradiation. 1 genotype consisting of a combination of 5 polymorphic loci is selected. Frequency of occurrence of this genotype in 1 group makes 16,9 % (14/83), in 2 group - 1,0 % (1/100), in 3 group - 0,84 % (1/119). At its carriers in conditions of low-dose irradiation more than in 20 times the risk of occurrence of MNG (OR between 1 and 3 groups (95 % CI) = 24,14 (3,21-502,64) increases; p = 0,0000581, taking into account the correction of Bonferroni р = 0,01046).
ALLО- AND SYMPATRIC SIBLING SPECIES OF SACCHARОMYСES CEREVISIAE: DNA-DNA REASSOCIATION
Authors: Mikhailova Yuliya V , Castello Sofia , Naumova Elena S , Naumov Gennadiy I 
Published: September 2017

Precise DNA-DNA reassociation data were obtained for new biological species S. cariocanus, S. kudriavzevii and S. mikatae, for the first time. The three species showed 25-51 % - of DNA-DNA reassociation with one another and with the known species S. cerevisiae, S. bayanus and S. paradoxus.Only in the combination S. paradoxus × S. cariocanus there was 99 % DNA-DNA homology. Despite high DNA-DNA reassociation value, the two yeasts are genetically isolated: their hybrids are sterile forming non-viable meiotic products (ascospores). Having four reciprocal translocations in its karyotype, S. cariocanus represents species in statu nascendi.
Variability of flax characters, associated with fibre formation, and environmental influence on their expression
Authors: Brach Nina B , Sharov Ivan Ya , Pavlov Andrey V , Porokhovinova Elisaveta A 
Published: September 2017

In this work the results of long-term evaluation of lines of flax genetic collection created in VIR are generalized. A wide variability of fibre productivity and quality characters, and also stability of their manifestation in varying environment is revealed. The genotypes possessing contrast traits of productivity, quality and stability of their display in different years are distinguished. Dependence of correlations between the evaluated characters on the environment parameters is detected. The described lines can serve as a material for profound studying of physiological processes of fibre formation, for the analysis of traits inheritance and successful breeding.
EXPRESSION OF RECOMBINANT RECEPTOR PROTEINS SYM10 AND SYM37 PISUM SATIVUM INVOLVED IN PERCEPTION OF LIPO-CHITOOLIGOSACCHARIDES NOD FACTORS
Authors: Dolgikh Elena A , Leppyanen Irina V , Zhukov Vladimir A , Tsyganov Viktor E , Tikhonovich Igor A 
Published: September 2017

In the legume-Rhizobium interaction Nod factors emanating from rhizobia trigger a complex of specific responses in epidermis, pericycle and root cortex of the plant, thereby providing the basis for subsequent bacterial entry and organogenesis of root nodules. Since Nod factors are biologically active at pico-nanomolar concentrations and their activity depends on Nod factor structural features, it suggests the presence of high affinity receptors to these molecules. Genetic analysis of pea mutants allowed to identify genes that are essential for symbiosis development and among of them the PsSym10 and PsSym37. These genes are predicted to encode LysM-receptor-like kinases with LysM motifs in extracellular domain (LysM-RLKs). These proteins may be potential receptors to Nod factors. However experimental evidence of Nod factor binding to the putative receptors is needed to confirm the biochemical function of receptors. Mainly, it depends on the problems with receiving of membrane receptors. In this work the heterologous expression of SYM10 and SYM37 was conducted in bacterial cells. We have also optimized the conditions for recombinant proteins purification and obtained specific antibodies for next immunoenzyme analysis of two LysM-RLKs in legume plants.
Association of apoprotein A-1 genetic variants with atherosclerosis development in Saint-Petersburg
Authors: Miroshnikova Valentina V , Rodygina Tatyana I , Demina Ekaterina P , Kuryanov Pavel S , Urazgildeeva Soreya A , Gurevich Victor S , Schwarzman Alexander L 
Published: September 2017

Apoprotein A-1 is a major protein in antiaterogenic high density lipoproteins and it is one of key proteins regulating reverse cholesterol transport. In this study we have investigated association of APOA1 gene polymorphism with atherosclerosis development among Saint-Petersburg population. Allelic frequencies of polimorphic variants (-75)G/A and 83C/T of APOA1 gene were determined in the group of patients with angiographically proven aterosclerosis and in control group. Allele 83T of the APOA1 gene is associated with lower risk of atherosclerosis development among Saint-Petersburg population.
Analysis of the association of polymorphic markers of dopaminergic (DRD2/ANKK1) and serotonergic (HTR2A) systems in the brain with the personality traits of adolescents
Authors: Barsky Vladimir I , Aksenova Marina G , Kozlova Olga B , Kirillov Andrey V , Demin Andrey A , Ilinykh Lubov M , Rapoport Irina K , Asanov Aliy U 
Published: September 2017

The studies of personality characteristic of young adolescents caused by their genetic predispositions have become very essential in recent years. In our study we have investigated the association of dopamine (D2) and serotonin (2A) receptor genes with personality traits in 360 young adults. We have found the influence of the combination of DRD2/ANKK1 and HTR2A genes on the personality traits measured on Rule-Consciousness and Self-Reliance scales in girls. The significant statistical difference on Self-control scale was established for polymorphism TaqIA of gene DRD2/ANKK1 in boys. Additionally, the differences on Social Boldness scale were observed after stratification of genotypes DRD2/ANKK1 and HTR2A.
MOLECULAR-GENETIC BASIS OF HIGHER PLANTS TOLERANCE TO, AND ACCUMULATION OF, CADMIUM
Authors: Kulaeva Olga A , Tsyganov Viktor E 
Published: September 2017

Cadmium (Cd) is one of the most wide-ranged and dangerous pollutants for all living organisms, including plants. At present time the intensive studies of mechanisms of Cd accumulation in plant tissues and plant tolerance to its toxic influence are performed. Data about variation of Cd tolerance and accumulation traits in natural populations of hyperaccumulators species as well as important crops were obtained. A series of mutants with changed sensitivity to Cd was obtained. In recent decade several classes of proteins involving in cell responses to Cd ions were revealed. An important role of microRNA in plant adaptation to Cd was recently demonstrated. Studies of molecular-genetic mechanisms of Cd accumulation and plant tolerance to it are theoretical basis for development of phytoremediation technologies of soil contaminated with heavy metals and breeding of crop varieties with decreased Cd accumulation.
THE GENETICS OF CHLOROPHYLL BIOSYNTHESIS: LIGHT-INDEPENDENT AND LIGHT-DEPENDENT PATHWAYS
Authors: Chekunova Elena M 
Published: September 2017

The review summarizes contemporary genetical, molecular biological and biochemical data on the two protochlorophyllide oxidoreductases (POR), enzymes responsible of light-dependent (LPOR) and dark-operative (DPOR) protochlorophyllide reduction. Evolutionary aspects of origin and functioning of these enzymes are also discussed. The main focus of this review will be the genetics of archaic dark chlorophyll biosynthesis.
MOLECULAR VARIATION AND PHYLOGEOGRAPHY OF THE MOSS CAMPION SILENE ACAULIS (L.) JACQ. (CARYOPHYLLACEAE) IN THE NORTH OF EUROPE AND THE SVALBARD ARCHIPELAGO
Authors: Mikhaylova Yuliya Vladimirovna , Gusarova Galina L , Brochmann Christian 
Published: September 2017

Molecular variation in 49 populations of Silene acaulis was studied using AFLP multilocus markers. Data obtained support postglacial colonization of the North the from southern European mountain refugia. Most of the northern European populations are characterized by lower genetic diversity and poor genetic structure, as compared to the southern mountain populations. High Arctic archipelago Shpitsbergen have been colonized by Silene acaulis from several sources, of which the most significant is eastern Greenland.
CHANGE OF POLYMORPHISM LEVEL IN COLORADO POTATO BEETLE POPULATIONS IN THE SOUTHERN URALS
Authors: Udalov Maksim B , Benkovskaya Galina V 
Published: September 2017

Data has been presented by the changing of phenetic structure of Colorado potato beetle populations in the Southern Urals (territory of Bashkortostan). The reduction of phenetic polymorphism level during the period of 1994–2002 has been noted. The results of laboratory toxicological experiments allow considering the selective effects of insecticides as one of main factors of intrapopulation polymorphism level decreasing.
THE ROLE OF POLYPLOIDY IN ADAPTATION AND SETTLING OF STEPPE SHRUBS IN CENTRAL ASIA
Authors: Ekimova Natalia V , Muratova Elena N , Silkin Pavel P 
Published: September 2017

Chromosome numbers for some species of Central Asia steppe shrubs are given: Rhamnus erythroxylon Pall. (Rhamnaceae), Caragana buriatica Peschk. (Fabaceae), Amygdalus pedunculata Pall., Armeniaca sibirica (L.) Lam. (Rosaceae), Atraphaxis pungens (Bieb.) Jaub. et Spach. and A. frutescens (L.) C. Koch (Polygonaceae). Chromosome numbers of some species were determined for the first time. Comparative analysis of adaptive properties of polyploid and diploid species has been conducted. It was established that natural polyploids are more adaptive to existence in extreme conditions. They possess by high potential for survival and characterized by more high level of intraspecific polymorphism, abundant flowering and fruitification, ability of propagate both generative and vegetative means, high seed germination.
Polymorphism of mtDNA in St Petersburg population of Adalia bipunctata and its relation with infection by symbiotic bacterium Spiroplasma
Authors: Zakharov-Gezekhus Ilya A , Shaikevich Elena V 
Published: September 2017

Polymorphism of the mtDNA gene COI was studied in a St. Petersburg population of two spot ladybird Adalia bipunctata and analyzed in relation with the presence of a symbiotic bacterium Spiroplasma. Variable nucleotide sequences in the middle part of the gene COI formed 13 mitotypes. 84 ladybirds were studied, 21 of these were found to be infected by Spiroplasma. Mean pairwise difference of nucleotides in the COI sequence was 0.001 for uninfected and 0.020 for infected individuals, thus mtDNA polymorphism was considerably higher among uninfected ladybirds compared with infected ones.
ASSOCIATION OF DNA REPAIR GENE POLYMORPHISM WITH CHROMOSOMAL ABERRATIONS IN THE HUMAN LYMPHOCYTES
Authors: Minina Varvara I , Druzhinin Vladimir G , Lunina Anna A , Larionov Aleksey V , Volkov Alexey N , Golovina Tatyana A , Glushkov Andrey N 
Published: September 2017

Analysis of association between several DNA repair gene polymorphisms and the level of chromosomal aberrations (CAs) in lymphocytes was performed in two groups of teenagers: a group of 256 donors exposed to indoor radon and a control group of 94 donors. In the group of children with living conditions exposing them to high doses of radon ( 200 Bq/m3 ), the level of CAs shows a significant increase in the carrier of genotypes: hOGG1 Cys/Cys, hOGG1 Ser/Cys, ADPRT Ala/Ala and ADPRT Val/Ala. Furthermore there were no significant associations between level of CA and Arg194Trp, Arg280His, Arg399Gln polymorphisms of the XRCC1 and Asp148Glu polymorphism of the APE1 found.
Evolution of micro-symbionts of cultured plants: experimental and mathematical models
Authors: Provorov Nikolay A , Vorobyov Nikolay I 
Published: September 2017

Formation of the cultured flora resulted in a decrease of plant symbiotic potential based on interactions with beneficial microorganisms. This decrease leads to transformation of plant micro-symbionts into the non-adaptive forms caused by: а) blocking the selection in favor of mutualistic strains; б) horizontal gene transfer in the microbial communities resulted in formation of virulent “symbiotic cheaters”. Mathematical simulation suggests that these tendencies may be overcome by formation of the optimal population structures in symbiotic system which should possess a high integrity and specificity of partners’ interactions as well as by a tolerance to the invasions of non-active aboriginal strains from the local soil populations.
The molecular basis for construction of highly productive ecologically sustainable agrocenoses
Authors: Tikhonovich Igor A , Provorov Nikolay A 
Published: September 2017

The methodology is suggested for analyzing the variability and heredity in super-organism genetic systems of different complexity ranging from two-component plant-microbe and animal-microbe symbiosis to the endosymbiotic and soil microbial communities. The approaches based on symbiogenetics and metagenomics may be used for development of highlyproductive ecologically sustainable agrocenoses based on the substitution of agrochemicals (mineral fertilizers, pesticides) by the microbial preparations. A possibility is emphasized to use the natural analogs of agrocenoses for development of models to implement the directed construction and improvement of productivity in the sustainable agricultural systems.
INTERSPECIES HYBRIDS OF RED CURRANTS (RIBES ATROPURPUREUM × RIBES HISPIDULUM) ON THE SALAIR RIDGE
Authors: Gorbunov Aleksey B , Padutov Vladimir E , Baranov Oleg U 
Published: September 2017

Interspecies hybrids Ribes atropurpureum × Ribes hispidulum occurring on the Salair ridge were revealed and studied on the basis of assessment of their morphological traits and molecular and genetic analysis.