bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oesophageal replacement with stomach: A personal series and review of published experience
Authors: Awad Karim , Jaffray Bruce 
Published: August 2017

AimTo describe the outcomes of oesophageal replacement using stomach in children.MethodsAll children undergoing oesophageal replacement in a regional centre were prospectively recorded in a customised database and subjected to continual follow up. Complications within 30 days were classified as early, and all other complications were classified as late. Outcomes were related to a comprehensive analysis of published experience where studies were classified as having long‐term follow up if the median duration exceeded 5 years.ResultsTen children underwent oesophageal replacement using the stomach between 1998 and 2016. Indications were oesophageal atresia (6), caustic ingestion (2), foreign body ingestion (1) and oesophageal hamartoma (1). Two children died at 2 and 7 months after gastric transposition. All survivors are under review, with a median follow up of 8.5 years (range 3–14 years).Complications occurred in every case. Among survivors, three had early complications and eight had late complications. Early complications included anastomotic leak (2) and lung compression by stomach (1). Late complications were anaemia (8), anastomotic stricture (7), oesophagitis (5), dumping syndrome (2), perforation of a jejunostomy (1), para‐gastric hiatal hernia (1), gastric outlet obstruction (1), Barrett's oesophagus (1), prolonged inability to swallow (1) and recurrent lower respiratory tract infections (1). Among 57 publications, only three achieved complete long‐term follow up. The incidence of reported complications was higher when follow up was complete.ConclusionsOesophageal replacement by gastric transposition in children leads to serious chronic morbidity. Published experience masks this because of incomplete and short follow up.
The effects of one versus two episodically oriented practice narratives on children's reports of a repeated event
Authors: Danby Meaghan C. , Brubacher Sonja P. , Sharman Stefanie J. , Powell Martine B. 
Published: August 2017

PurposePrevious research has found that children's reports of repeated events can be influenced by the presence and type of narrative practice in which they engage immediately prior to substantive recall. In particular, children's reports have been shown to benefit from practice providing narratives about an autobiographical repeated event. A gap remains, however, with regard to understanding whether practice narrating one episode of a repeated event encourages children to think about unique features of specific episodes, or whether practice of two episodes is required. The current study addressed this gap.MethodsFive‐ to nine‐year‐olds (N = 167) experienced four classroom activity sessions and were later interviewed. Children provided a practice narrative about either one or two episodes of an autobiographical repeated event prior to discussing individual episodes of the activities.ResultsOlder children recalled more details from the activities when they had practised recalling two episodes compared to one episode. Younger children did not benefit from the second episodic practice. Many similarities were observed across conditions for children of all ages.ConclusionsOlder children were likely receptive to the subtle differences between conditions because of their advanced cognitive abilities. Interviewers may assist older children to recall a larger amount of information if they first provide practice recalling two episodes of an autobiographical repeated event. However, a practice narrative about one episode may be sufficient to assist many children should two‐episode practice be unfeasible or interviewees are too young to benefit from recall of a second episode.
A functional imaging study of germinating oilseed rape seed
Authors: Munz Eberhard , Rolletschek Hardy , Oeltze‐Jafra Steffen , Fuchs Johannes , Guendel André , Neuberger Thomas , Ortleb Stefan , Jakob Peter M. , Borisjuk Ljudmilla 
Published: August 2017

Summary

Germination, the process whereby a dry, quiescent seed springs to life, has been a focus of plant biologist for many years, yet the early events following water uptake, during which metabolism of the embryo is restarted, remain enigmatic.

Here, the nature of the cues required for this restarting in oilseed rape (Brassica napus) seed has been investigated. A holistic in vivo approach was designed to display the link between the entry and allocation of water, metabolic events and structural changes occurring during germination. For this, we combined functional magnetic resonance imaging with Fourier transform infrared microscopy, fluorescence‐based respiration mapping, computer‐aided seed modeling and biochemical tools.

We uncovered an endospermal lipid gap, which channels water to the radicle tip, from whence it is distributed via embryonic vasculature toward cotyledon tissues. The resumption of respiration is initiated first in the endosperm, only later spreading to the embryo. Sugar metabolism and lipid utilization are linked to the spatiotemporal sequence of tissue rehydration.

Together, this imaging study provides insights into the spatial aspects of key events in oilseed rape seeds leading to germination. It demonstrates how seed architecture predetermines the pattern of water intake, which sets the stage for the orchestrated restart of life.
A longitudinal analysis of financial loss, anxiety, destructive conflict, and relational maintenance
Authors: MEROLLA ANDY J. 
Published: August 2017

AbstractThis study examined how destructive conflict behavior and relational maintenance behavior are linked to financial loss in adult romantic relationships. Based on conservation of resources (COR) theory, it was hypothesized that financial loss indirectly (through anxiety) predicts increased destructive conflict behavior and decreased relational maintenance behavior. Across three waves of data, results indicated that financial loss significantly predicted passive‐destructive conflict behavior (i.e., neglect) through anxiety. Financial loss did not, however, directly or indirectly predict active‐destructive conflict behavior (i.e., exit). Financial loss negatively predicted relational maintenance behavior, but that effect was found only from Wave 1 to Wave 2 and was not mediated by anxiety. Overall, results support COR theory and identify some of the specific relational consequences of financial difficulty.
Thermal limits to survival and activity in two life stages of false codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae)
Authors: Terblanche John S. , Mitchell Katherine A. , Uys Wilmari , Short Clancy , Boardman Leigh 
Published: August 2017

AbstractThe present study examines life stage‐related variation in the thermal limits to activity and survival in an African pest, the false codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae). Thermal tolerance, including the functional activity limits of critical thermal maxima and minima (CTmax and CTmin respectively), upper and lower lethal temperature, and the effect of heat and cold hardening (short‐term acute plasticity), is measured across a diverse range of low or high temperature stress conditions in both larvae and adults. We also report the sum of inducible and cognate forms of the amounts of heat shock protein 70 (HSP70) as an explanatory variable for changes in thermotolerance. The results show that the larvae have high variability in CTmax and CTmin at different ramping rates and low levels of basal (innate) thermal tolerance. By contrast, the adults show high basal tolerance and overall lower variability in CTmax and CTmin, indicating lower levels of phenotypic plasticity in thermotolerance. HSP70 responses, although variable, do not reflect these tolerance or survival patterns. Larvae survive across a broader range of temperatures, whereas adults remain active across a broader range of temperatures. Life stage‐related variation in thermal tolerance is most pronounced under the slowest (most ecologically‐relevant) ramping rate (0.06 °C min–1) during lower critical thermal limit experiments and least pronounced during upper thermal limit experiments. Thus, the ramping rate can hinder or enhance the detection of stage‐related variation in thermal limits to activity and survival of insects.
Attraction range of a sex pheromone trap for the damson‐hop aphidPhorodon humuli(Hemiptera:Aphididae)
Authors: Campbell Colin A. M. , Hardie Jim , Wadhams Lester J. 
Published: August 2017

AbstractThe attraction range of olfactory response by winged female gynoparae (autumn migrants that give birth to oviparae, the sexual females) and male damson–hop aphidsPhorodon humuli(Schrank) is investigated in field experiments over 2 years by analyzing the spatial patterns of catches in concentric circles of yellow‐painted traps (60 in total) around a central trap releasing the species' sex pheromone, (1RS,7S,7aS)‐nepetalactol. Males are more likely than females to be found in the central trap, with 65.6% of the 1824 males caught there compared with 11.2% of 1346 females. Both morphs are more numerous in traps axial with the mean wind direction and centred on the pheromone‐release trap than at other angles. Males are approximately five‐fold more numerous in traps downwind than at similar distances upwind of the pheromone, showing that its presence stimulates landing. For males, the estimated active space of the lure extends 6 m downwind. Catches of females are equally numerous up and downwind of the pheromone lure because females orienting on the axis of the pheromone source continue to respond to visual cues in their flight path if they overshoot the olfactory one. For females, the active space of a pheromone lure is less than 2 m downwind. It is unimportant for either morph whether the pheromone‐release trap is yellow or transparent. In these experiments, both morphs orient with, track and probably arrive in the pheromone source trap from at least 26 m, the distance to the nearest aphid‐infested hops.
Educational Alignment: Learning – Teaching Approaches as Influencing Factors
Authors: Kulhmann Lüdeke Angelika , Sánchez Zúñiga María Claudia 
Published: August 2017

<p>La implementación de innovaciones en programas académicos de medicina requiere evaluar los aspectos que cumplen papeles fundamentales dentro de la compleja relación del sistema estudiante-docente-contexto educativo. El objetivo del artículo es determinar si los objetivos de aprendizaje y las intervenciones educativas concuerdan con el alineamiento educativo. Este se puede evaluar explorando el abordaje del aprendizaje adoptado por los estudiantes (profundo <em>vs.</em> superficial) y el abordaje de enseñanza adoptado por los miembros del cuerpo docente (centrado en estudiantes <em>vs.</em> centrado en docentes). Un alineamiento entre estos factores asegura una mayor calidad del programa educativo, que fomenta el desarrollo de profesionales con capacidad de autoestudio y autorregulación, en busca de aprendizajes significativos continuos a lo largo de la vida. Así mismo, programas en proceso de cambios curriculares deben evaluar estos aspectos que antes no estaban en los currículos tradicionales. </p>
Complete Genome Sequence and Annotation of the Staphylococcus aureus Strain HG001
Authors: Caldelari Isabelle , Chane-Woon-Ming Béatrice , Noirot Céline , Moreau Karen , Romby Pascale , Gaspin Christine , Marzi Stefano 
Published: August 2017

ABSTRACT

Staphylococcus aureus
is an opportunistic Gram-positive pathogen responsible for a wide range of infections from minor skin abscesses to life-threatening diseases. Here, we report the draft genome assembly and current annotation of the HG001 strain, a derivative of the RN1 (NCT8325) strain with restored
rbsU
(a positive activator of SigB).
Modelling the health impact and cost-effectiveness of lymphatic filariasis eradication under varying levels of mass drug administration scale-up and geographic coverage
Authors: Stone Christopher M , Kastner Randee , Steinmann Peter , Chitnis Nakul , Tanner Marcel , Tediosi Fabrizio 
Published: August 2017

BackgroundA global programme to eliminate lymphatic filariasis (GPELF) is underway, yet two key programmatic features are currently still lacking: (1) the extension of efforts to all lymphatic filariasis (LF) endemic countries, and (2) the expansion of geographic coverage of mass drug administration (MDA) within countries. For varying levels of scale-up of MDA, we assessed the health benefits and the incremental cost-effectiveness ratios (ICERs) associated with LF eradication, projected the potential savings due to decreased morbidity management needs, and estimated potential household productivity gains as a result of reduced LF-related morbidity.MethodsWe extended an LF transmission model to track hydrocele and lymphoedema incidence in order to obtain estimates of the disability adjusted life years (DALYs) averted due to scaling up MDA over a period of 50 years. We then estimated the ICERs and the cost-effectiveness acceptability curves associated with different rates of MDA scale-up. Health systems savings were estimated by considering the averted morbidity, treatment-seeking behaviour and morbidity management costs. Gains in worker productivity were estimated by multiplying estimated working days lost as a result of morbidity with country-specific per-worker agricultural wages.ResultsOur projections indicate that a massive scaling-up of MDA could lead to 4.38 million incremental DALYs averted over a 50-year time horizon compared to a scenario which mirrors current efforts against LF. In comparison to maintaining the current rate of progress against LF, massive scaling-up of MDA—pursuing LF eradication as soon as possible—was most likely to be cost-effective above a willingness to pay threshold of US$71.5/DALY averted. Intensified MDA scale-up was also associated with lower ICERs. Furthermore, this could result in health systems savings up to US$483 million. Extending coverage to all endemic areas could generate additional economic benefits through gains in worker productivity between US$3.4 and US$14.4 billion.ConclusionsIn addition to ethical and political motivations for scaling-up MDA rapidly, this analysis provides economic support for increasing the intensity of MDA programmes.
Accumulation of Smooth Muscle 22α Protein Accelerates Senescence of Vascular Smooth Muscle Cells via Stabilization of p53 In Vitro and In Vivo
Authors: Miao Sui-Bing , Xie Xiao-Li , Yin Ya-Juan , Zhao Li-Li , Zhang Fan , Shu Ya-Nan , Chen Rong , Chen Peng , Dong Li-Hua , Lin Yan-Ling , Lv Pin , Zhang Dan-Dan , Nie Xi , Xue Zhen-Ying , Han Mei 
Published: August 2017

Objective—
Smooth muscle (SM) 22α, an actin-binding protein, displays an upregulated expression as a marker during cellular senescence. However, the causal relationship between SM22α and senescence is poorly understood. This study aimed to investigate the role of SM22α in angiotensin II (Ang II)–induced senescence of vascular smooth muscle cells (VSMCs).

Approach and Results—
We prepared a model of VSMC senescence induced by Ang II and found that the expression of SM22α in VSMCs was increased in response to chronic Ang II treatment. Overexpression of SM22α promoted Ang II–induced VSMC senescence, whereas knockdown of SM22α suppressed this process. Moreover, this effect of SM22α was p53 dependent. Increased SM22α protein obstructed ubiquitination and degradation of p53 and subsequently improved its stability. Furthermore, SM22α inhibited phosphorylation of Mdm2 (mouse double minute 2 homolog), an E3 ubiquitin-protein ligase, accompanied by a decreased interaction between Mdm2 and p53. Using LY294002, a PI3K/Akt inhibitor, we found that PI3K/Akt-mediated Mdm2 phosphorylation and activation was inhibited in senescent or SM22α-overexpressed VSMCs, in parallel with decreased p53 ubiquitination. We further found that SM22α inhibited activation of PI3K/Akt/Mdm2 pathway via strengthening actin cytoskeleton. In the in vivo study, we showed that the disruption of SM22α reduced the increase of blood pressure induced by Ang II, associated with decreased VSMC senescence through a mechanism similar to that in VSMCs in vitro.

Conclusions—
In conclusion, these findings suggest that the accumulation of SM22α promotes Ang II–induced senescence via the suppression of Mdm2-mediated ubiquitination and degradation of p53 in VSMCs in vitro and in vivo.
The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments)
Authors: Percie du Sert Nathalie , Alfieri Alessio , Allan Stuart M , Carswell Hilary VO , Deuchar Graeme A , Farr Tracy D , Flecknell Paul , Gallagher Lindsay , Gibson Claire L , Haley Michael J , Macleod Malcolm R , McColl Barry W , McCabe Christopher , Morancho Anna , Moon Lawrence DF , O’Neill Michael J , Pérez de Puig Isabel , Planas Anna , Ragan C Ian , Rosell Anna , Roy Lisa A , Ryder Kathryn O , Simats Alba , Sena Emily S , Sutherland Brad A , Tricklebank Mark D , Trueman Rebecca C , Whitfield Lucy , Wong Raymond , Macrae I Mhairi 
Published: August 2017

Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).
Frontline Science: Leishmania mexicana amastigotes can replicate within neutrophils
Authors: Hurrell Benjamin P , Beaumann Manon , Heyde Sandrina , Regli Ivo B , Müller Andreas J , Tacchini-Cottier Fabienne 
Published: August 2017

Abstract
Cutaneous leishmaniasis is a neglected tropical disease, causing a spectrum of clinical manifestations varying from self-healing to unhealing lesions that may be very difficult to treat. Emerging evidence points to a detrimental role for neutrophils during the first hours following infection with many distinct Leishmania species (spp.) at a time when the parasite is in its nonreplicative promastigote form. Neutrophils have also been detected at later stages of infection in unhealing chronic cutaneous lesions. However, the interactions between these cells and the replicative intracellular amastigote form of the parasite have been poorly studied. Here, we show that Leishmania mexicana amastigotes are efficiently internalized by neutrophils and that this process has only a low impact on neutrophil activation and apoptosis. In neutrophils, the amastigotes were found in acidified vesicles. Furthermore, within cutaneous unhealing lesions, heavily infected neutrophils were found with up to 6 parasites per cell. To investigate if the amastigotes could replicate within neutrophils, we generated photoconvertible fluorescent parasites. With the use of flow cytometry imaging and time-lapse microscopy, we could demonstrate that a subset of parasites replicated within neutrophils. Overall, our data reveal a novel role for neutrophils that can act as a niche for parasite replication during the chronic phase of infection, thereby contributing to disease pathology.
ANÁLISIS DE LA VIABILIDAD EN LA IMPLEMENTACIÓN DE VANET SOBRE EL MEDIO DE TRANSPORTE TRANSMILENIO
Authors: Gelvez García Nancy Yaneth , López Sarmiento Danilo Alfonso , Villanueva Ocampo Bayron Fabio 
Published: August 2017

En este artículo, se analiza la viabilidad de implementar una red móvil ad hoc que soporte servicios informativos, aplicado al sistema de transporte masivo Transmilenio en la ciudad de Bogotá (Colombia). Para conseguir este fin, se hace uso de un simulador de redes telemáticas como es NS-2 (Network Simulator), un simulador de redes de vehículos llamado SUMO (Simulation of Urban Mobility) y MOVE (Mobility model generator for Vehicular networks) una interfaz de conexión entre ambos simuladores. Finalmente, se analiza los problemas que acarrea implementar la red.
DISEÑO E IMPLEMENTACIÓN DE UN PROTOTIPO PARA EL REGISTRO Y VERIFICACIÓN DE ACTIVOS FIJOS UTILIZANDO PLATAFORMA ANDROID Y TECNOLOGÍA NFC
Authors: Acosta López Alberto , Manzano González Dago José , Martínez Morales Carlos , Ponce Fajardo Iván 
Published: August 2017

Este documento plantea una propuesta de prototipo e implementación de una aplicación, construida en plataforma Android utilizando tecnologías NFC. La motivación para la elaboración de la aplicación es la problemática de la validación y control de flujo de activos fijos, bastante común en la actualidad. Con este desarrollo se busca proporcionar una solución a la tarea del registro y validación de entrada y salida de elementos físicos, que estarán relacionados con información almacenada en una base de datos donde se asociarán con una persona, en un lugar en específico. En el desarrollo de la aplicación se implementó una arquitectura de software basada en cliente-servidor contando con elementos tipo web y móvil, y acoplando diferentes tecnologías que convergen a un único servicio. Como trabajo futuro se destacara la posibilidad de extender la aplicación a cualquier organización que la requiera, también deben explorarse nuevas metodologías de seguridad en general para las etiquetas NFC que permitan generar una capa de seguridad eficiente y robusta. Finalmente, se obtiene como resultado una versión funcional del aplicativo probada en la Universidad Distrital Francisco José de Caldas de la ciudad de Bogotá en Colombia para el control de entrada y salida de dispositivos electrónicos, con resultados satisfactorios.
ESTUDIO COMPARATIVO DEL DESEMPEÑO DEL PROTOCOLO IP Y EL PROFIBUS DE TIPO PA, CON BASE EN EL DISEÑO E IMPLEMENTACIÓN DE UN SISTEMA DE COMUNICACIÓN ENTRE ELLOS MEDIANTE UNA APLICACIÓN GENERAL USANDO SISTEMAS PROGRAMABLES DE DESARROLLO
Authors: Romero Rodolfo , Caballero Cristian Camilo , López Sarmiento Danilo Alfonso 
Published: August 2017

El artículo presenta el desarrollo de una aplicación de interconexión entre los protocolos Profibus e IPv4, pretendiendo implementar un bus de campo tipo PA sin seguridad intrínseca y que sea monitoreado desde Internet mediante un servicio web llamado ThingSpeak, el cual sirve como base para la elaboración del estudio concerniente a los protocolos enunciados al inicio, apreciando las limitaciones de los sistemas industriales y sus tendencias hacia el futuro, donde llegarán a fusionarse con el protocolo Ethernet, dando origen a nueva generación de la estandarización de buses de campo.
APLICACIÓN DE LA REALIDAD AUMENTADA A LOS PROCEDIMIENTOS ORTODÓNTICOS
Authors: Ruiz Gómez Adiela , Paez Paez Jaime Alberto , Cortes Mendez Jairo Augusto , Gonzales Bernal Maria Alejandra 
Published: August 2017

Una empresa que adopta la gestión del conocimiento en su modelo OSCL garantiza que gana en identidad y en arquitectura empresarial mediante la conducción del flujo organizacional en estados de perturbación o turbulencia, logrando arquetipos de labor disciplinada en estados de movilidad estática o dinámica y orden simple o complejo desde los cuales se valora direcciona y conduce la empresa hacia la cultura del éxito desde el desarrollo de los sujetos hacia y en el desarrollo empresarial impactando y estimulando el desarrollo social