bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between the Gut Microbiota and Sleep Disorder in Post-COVID-19 Patients Following Omicron Wave After Nearly 1-Year Recovery
Authors: Ming Xiaoping , Cai Wanyue , Zhou Aina , Sun Xiaoqi , Fang Lucheng , Hao Bin , Hu Yifan , Wu Jianghao , Cai Weisong , Chen Xiong 
Published: October 2024

Abstract

Background and objective:
The SARS-CoV-2 Omicron variant has exacerbated public health threats due to its notably increased transmission rate. Previous studies have suggested that the gut microbiota becomes impaired after infection. Given that impaired gut microbiota can trigger numerous neurological disorder and the sleep disorder is one of the most common symptoms post-COVID-19, we suggest that long-term sleep disorder symptoms of COVID-19 may be related to intestinal microbiota disorder in these patients. However, gut microbiota profiling in long-COVID patients, particularly those with sleep disorder in the outpatient setting, is still limited, and the impact of the Omicron variant on the recovery of the gut microbiota remains to be determined.
Methods
We performed metagenomic sequencing of the gut microbiota of eight patients who developed new-onset sleep disorder after Omicron infection, before and after the Omicron wave, to evaluate the impact of sleep disorder on gut recovery after SARS-CoV-2 Omicron variant infection.
Results
Our results suggest that the post-COVID-19 gut microbiota is characterized by a decrease in commensal microbes, an increase in opportunistic pathogens, and alterations in metabolic functions, particularly in amino acid metabolism.
Conclusion
These findings provide preliminary evidence that the gut microbiota may not be fully restored in post-COVID-19 patients with sleep disorder following the Omicron wave, even after nearly a year of recovery. This highlights the importance of continuously monitoring gut health in people who have recovered from the Omicron wave.
The Mediating Role of Sleep Disturbances in the Relationship Between Obsessive-Compulsive Symptoms and Suicidal Ideation: The Moderating Effect of Childhood Trauma
Authors: Wang shaoxia , Zhang Xiang , Wang Yanrong , Wu Chujun , Fang Jianqun 
Published: October 2024

Abstract

Objective: This study aims to investigate the relationships and underlying mechanisms among obsessive-compulsive symptoms, sleep disturbances, childhood trauma, and suicidal ideation in patients with obsessive-compulsive disorder (OCD).
Methods: The study employed the Yale-Brown Obsessive Compulsive Scale (Y-BOCS), Pittsburgh Sleep Quality Index (PSQI), Childhood Trauma Questionnaire (CTQ), and a self-assessment scale for suicidal ideation (SIOSS) to survey 107 patients with OCD.
Results: Obsessive-compulsive symptoms significantly positively affected sleep quality (β = 0.31, t = 10.67, p < 0.001). Both sleep disturbances (β = 0.20, t = 7.86, p < 0.001) and obsessive-compulsive symptoms (β = 0.39, t = 14.65, p < 0.001) significantly predicted suicidal risk. Further analysis revealed that sleep disturbances served as a significant mediator between obsessive-compulsive symptoms and suicidal ideation (mediation effect value = 0.08, 95% CI = [0.22, 0.28]). Childhood trauma moderated the mediating effect, intensifying the positive impact of obsessive-compulsive symptoms on sleep disturbances and suicidal ideation as levels of childhood trauma increased.
Conclusion: Sleep disturbances mediate the relationship between obsessive-compulsive symptoms and suicidal ideation, with this mediation significantly influenced by the presence of childhood trauma. These findings underscore the importance of considering early life experiences and psychophysiological backgrounds when assessing and intervening in the suicidal risk of OCD patients.
Action Perception, Motor Imagery and Execution of Hand Movements in Autistic and Non-Autistic Adults
Authors: Bai Ying , Brillinger Molly , Karlinsky April D. , Poliakoff Ellen , Welsh Timothy N. , Gowen Emma 
Published: October 2024

Abstract

Action perception, execution, and imagery share motor-cognitive processes. Given prevalent motor coordination difficulties in autism, the processes of action perception and imagery may also be altered. This study investigated whether autistic adults can engage in motor imagery by testing differences in executing, perceiving, and imagining hand movements between autistic and non-autistic adults. Twenty autistic individuals and twenty age- and IQ-matched controls completed execution, imagination, and perception tasks using a Fitts’ Law paradigm in an online session. For the execution and imagination tasks, participants performed or imagined making aiming movements between two targets. For the action perception task, participants indicated whether they could perform as accurately as the movements in presented videos. Target size and distance were manipulated into three difficulty levels and systematically varied across all tasks. Results showed a similar Fitts’ Law relationship for both groups, with significant positive correlations between movement times and difficulty level. Movement times were longest in the imagination task and shortest in the perception task for both groups. These findings suggest motor imagery processes are relatively intact in autistic adults, opening the possibility of using motor imagery as a therapy for motor coordination difficulties in autistic individuals.
Monolaurin inhibits Antibiotic-resistant Staphylococcus aureus in Patients with Atopic Dermatitis
Authors: Laowansiri Matchima , Suwanchote Supaporn , Wannigama Dhammika Leshan , Badavath Vishnu Nayak , Hongsing Parichart , Edwards Steven W , Suratannon Narissara , Chatchatee Pantipa , Lertpichitkul Pattamon , Rerknimitr Pawinee , Chantawarangul Karaked , Chatproedprai Susheera , Wananukul Siriwan , Thammahong Arsa , Plongla Rongpong , Chanachaithong Pattrarat , Chavasiri Warinthorn , Chatsuwan Tanittha , Chiewchengchol Direkrit 
Published: October 2024

Abstract

Frequent use of antibiotics increases the incidence of antimicrobial-resistant Staphylococcus aureus in atopic dermatitis (AD), which prompts the search for new treatments. Monolaurin is a chemical byproduct found in coconut oil and has anti-bacterial properties. This study aimed to investigate the inhibitory effect of monolaurin on antimicrobial-resistant S. aureus. Thirty children and thirty adults diagnosed with AD were recruited and swabbed at three different sites: lesion, non-lesion, and nasal mucosa. Methicillin resistance and high-level mupirocin resistance in S. aureus were identified using mecA and mupA PCR, respectively, whilst fusidic acid resistance were detected by fusA gene sequencing. The broth microdilution method and tetrazolium bromide assays were used for monolaurin susceptibility and cellular cytotoxicity, respectively. We show that S. aureus was frequently isolated from lesions of both children and adults with AD. One isolate of methicillin-resistant S. aureus (MRSA) harboring mecA, one isolate of mupirocin-resistant S. aureus harboring mupA, and four isolates of fusidic acid-resistant S. aureus with novel point mutations of fusA were found in the children group. In silico molecular docking showed that these mutants interacted weakly with fusidic acid, explaining the mechanism of drug resistance. Monolaurin inhibited these antimicrobial-resistant S. aureus isolates with a minimal inhibitory concentration of 2 µg/mL without cytotoxicity to cultured epidermal and dermal cells. These data show that monolaurin could potentially be used to inhibit antimicrobial-resistant S. aureus in AD patients.
LINC01432 binds to CELF2 in newly diagnosed multiple myeloma promoting short progression-free survival to standard therapy
Authors: Silva-Fisher Jessica , Mishra Richa , Thunuguntla Prasanth , Perkin Alani , Duraiyan Dhanusha , Bagwill Katelyn , Gonzales Savannah , Brizuela Vanessa , Daly Steve , Chang Yoon Jae , Abebe Mahdote , Rajana Yash , Wichmann Kelly , Bolick Catheryn , King Jaiyana , Fiala Mark , Fortier Julie , Jayasinghe Reyka , Schroeder Mark , Ding Li , Vij Ravi 
Published: October 2024

Abstract

Multiple Myeloma (MM) is an incurable form of cancer that arises from malignant plasma cells, with over 35,000 new cases diagnosed annually in the United States. While there are a growing number of approved therapies, MM remains incurable and nearly all patients will relapse and exhaust available treatments. Mechanisms for disease progression are unclear and little is known regarding the role of long non-coding RNAs (lncRNA) in mediating disease progression and response to treatment. Here, we used transcriptome sequencing to compare newly diagnosed MM (NDMM) patients who had short progression-free survival (PFS) to standard first-line treatment (PFS < 24 months) to patients who had prolonged PFS (PFS > 24 months). We identified 157 differentially upregulated lncRNAs with short PFS and focused our efforts on characterizing the most upregulated lncRNA, LINC01432. We investigated LINC01432 to show that its overexpression significantly increases cell viability and reduces apoptosis, while knockdown significantly reduces viability and increases apoptosis. Next, we show that LINC01432 directly interacts with the RNA binding protein, CELF2. Lastly, we showed that LINC01432-targeted locked nucleic acid antisense oligonucleotides reduce viability and increases apoptosis. In summary, this fundamental study identified lncRNAs associated with short PFS to standard NDMM treatment and further characterized LINC01432.
Association between C-reactive protein variability and cancer incidence: a longitudinal prospective cohort study
Authors: Chen Yue , Wang Yiming , Zheng Xin , Liu Tong , Liu Chenan , Lin Shiqi , Xie Hailun , Zhang Heyang , Shi Jinyu , Liu Xiaoyue , Ma Xiangming , Deng Li , Zhang Qingsong , Shi Hanping 
Published: October 2024

Abstract

Background
The association between inflammation and cancer has been widely studied, but research on the relationship between the magnitude of inflammatory fluctuations and cancer remains limited. Therefore, this study aims to investigate the association between C-reactive protein (CRP) variability and the occurrence of cancer.
Methods
A total of 42,514 participants were included, and their CRP levels were measured over a 4-year period. We used the coefficient of variation (CV) of CRP to quantify the variability in inflammation. Cox regression analysis was used to assess the association between CRP variability and cancer risk after adjusting for potential confounding factors.
Results
High CV of CRP significantly associated with reduced cancer risk (hazard ratio (HR) = 0.84; 95% CI: 0.75–0.94, P = 0.003). Participants with high CRP and low CV had a significantly increased risk of cancer (HR = 1.42; 95% CI: 1.18–1.70, P < 0.001). In the population with long-term stable CRP levels, there is a significant association between the CV and the risk of cancer (HR = 0.81; 95% CI: 0.72–0.92, P = 0.001). Furthermore, the association between the CV and lung cancer was most pronounced. Sensitivity analyses confirmed the stability of the association between CRP variability and cancer.
Conclusion
High CRP variability is significantly associated with reduced risk of cancer, particularly in the case of lung cancer. This provides a new perspective and evidence for exploring the relationship between inflammation and cancer.
Progression of Disease Within 24 Months (POD24) Based Prognostic Signature: A New Method for Personalized Risk Assessment in Patients with Mantle Cell Lymphoma
Authors: He Yizi , Wang Caiqin , Pan Tao , Cai Qingqing , Zhou Dehui , Zhang Huilai , Liang Rong , Zeng Dongfeng , Ye Haige , Liang Yun , Sun Xiuhua , Xiao Ling , Zhou Hui 
Published: October 2024

Abstract

Mantle cell lymphoma (MCL) exhibits significant biological and clinical heterogeneity, necessitating a refined prognostic model. According to the drawback of the models existed which are not truly define the complexity of the disease, we used the clinical and molecular data from nine medical centers of China to validate the predictive utility in progression of disease within 24 months (POD24), and also establish a novel prognostic risk model to predict the survival outcome of MCL patients. POD24 occurred in 37.7% of evaluable patients, with median over survival was 21 months (vs 122 months for those without POD24, P < 0.0001). The POD24-based risk model had the highest sensitivity to predict to predict survival with the most satisfying AUC value for risk score (AUC = 0.869). In conclusion, we confirm the obviously predictive performance of POD24 and established a novel risk model combined POD24 and clinical factors. Our new prognostic model might be helpful to effectively classify MCL patients with high-risk groups in terms of survival rate, which may help select high-risk MCL patients for more intensive treatment at time of relapse.
Early prediction of local tumor progression after ablation of colorectal liver metastases based on MRI radiomics
Authors: Zhu Xiucong , Zhu Jinke , Sun Chenwen , Zhu Fandong , Zhao Zhenhua 
Published: October 2024

Abstract

The aim of this study was to investigate a magnetic resonance imaging(MRI)-based model for early prediction of local tumor progression (LTP) after ablation of colorectal cancer liver metastases (CRLM).53 patients with CRLM ablation were enrolled in a total of 83 lesions. The lesions were divided into LTP group (n = 27) and non-LTP group (n = 56). Radiomics features were extracted from the patients' post ablation enhanced MRI arterial phase in the ablation zone, and around the ablation zone (0-5mm, 0-10mm, 5-15mm) to establish radiomics, clinical and combined models. Tumor size correlated with high LTP after ablation (p < 0.05). The 0–10 mm radiomics model around the ablation zone showed good performance, with an area under the curve (AUC) of 0.874 for the training set and 0.831 for the validation set. In addition, the diagnostic efficacy of the combined model of PAZ2 and AZ as well as the combined model of AZ-PAZ2 and clinical risk factors was superior, with an AUC of 0.896 and 0.919, respectively, for the training set, and AUC of 0.882 and 0.875, respectively, for the validation set. were 0.882 and 0.875 for the validation group, respectively. In conclusion, the combined postoperative ablation zone and peri-ablation zone radiomics model can early predict LTP after ablation in CRLM patients.
Ribosomal biogenesis factor, a novel biomarker for predicting progression-free survival in prostate cancer
Authors: Chen Zexiao , Fang Yutong , Zhang Jianhua 
Published: October 2024

Abstract

Background
Prostate cancer (PCa) is the second most common malignancy among men worldwide, with significant variability in incidence rates across different regions. Effective management of PCa is crucial, especially for advanced stages where the survival rates are notably low. Ribosome biogenesis (RB) plays a critical role in cancer cell proliferation, yet the specific function of the ribosomal biogenesis factor (RBIS) gene in PCa remains unexplored..
Methods
RNA sequencing data from the TCGA database and three GEO datasets were analyzed to assess RBIS expression in PCa. Clinicopathological features, survival rates, and drug sensitivity were evaluated in relation to RBIS expression. Gene co-expression and functional enrichment analyses were performed to investigate potential biological mechanisms. Additionally, immune cell infiltration and genetic alterations of RBIS were analyzed.
Results
RBIS expression was significantly elevated in PCa tissues compared to normal tissues. High RBIS expression correlated with adverse clinical outcomes, including advanced tumor stages and higher Gleason scores. Elevated RBIS levels were associated with poorer progression-free survival (PFS) and served as an independent prognostic marker. Co-expression analysis revealed that RBIS and its associated genes were involved in key cellular processes such as energy metabolism and protein synthesis. Furthermore, RBIS expression was linked to immune cell infiltration and drug sensitivity, indicating potential therapeutic implications.
Conclusion
RBIS emerges as a novel biomarker for the diagnosis and prognosis of PCa, with significant potential as a therapeutic target. Further research is needed to validate these findings and explore RBIS's role in clinical applications, aiming to improve PCa management and patient outcomes.
Hope and health promoting intention among stroke patients in China: the mediating role of self-efficacy
Authors: Chen Lei , Huang Tenjia , Cao Xi , Liu Yixin , Li Sheng , Qin Lihua 
Published: October 2024

Abstract

Introduction:
Stroke is one of the major diseases that threaten life and health globally, and reducing the risk of recurrence after stroke and promoting the recovery of physical and mental functions are significant challenges in solving the health problems of stroke patients. Compared to surgical and pharmacologic treatments, relatively few studies have focused on improving health promoting intention in stroke patients to enhance disease prognosis. This study aimed to explore the relationship between health promoting intention, hope, and self-efficacy.
Methods
From June 2023 to November 2023, a questionnaire survey was conducted among 521 stroke patients in the affiliated hospitals of Hunan University of Chinese Medicine using cluster sampling. The survey instruments included a general demographics questionnaire, Health-Promoting Lifestyle Scale II, General Self Efficacy Scale, and Herth Hope Index. Multiple regression analysis was used to explore the influencing factors of health promoting intention, and structural equation modeling was constructed to test the mediating role of self-efficacy between hope and health promoting intention.
Results
A total of 499 valid questionnaires were included. There was a significant positive correlation between health promoting intention, hope, and self-efficacy (p < 0.01). Hope (β = 2.812, p < 0.01), self-efficacy (β = 6.712, p < 0.001), and disease knowledge (β = 0.167, p < 0.001) significantly and positively influenced health promoting intention. Self-efficacy partially mediated the relationship between hope and health promoting intention [β = 0.258, 95%CI (0.126, 0.401)], with the indirect effect accounting for 41.55%.
Conclusion
This study confirmed the significant positive effect of hope on health promoting intention, with self-efficacy mediating between the two. Future research should explore other influences on health promoting intention to develop a comprehensive health promotion behavioral intervention program for stroke patients.
Trajectories of medical students’ empathy nowadays: A longitudinal study using a comprehensive framework of empathy
Authors: Carrard Valerie , Bourquin Céline , Berney Sylvie , Ranjbar Setareh , Schlegel Katja , Gaume Jacques , Bart Pierre-Alexandre , Mast Marianne Schmid , Preisig Martin , Berney Alexandre 
Published: October 2024

Abstract

Background. For more than a decade, the literature has been dominated by the notion that medical students may paradoxically lose their empathy during medical school. However, medical curricula have significantly evolved, and the question is whether this is still the case. The present study aimed to describe the trajectories of different dimensions of empathy from the beginning to the end of a six-year medical curriculum and explore the influence of different psychosocial and health-related factors.
Methods. In an open cohort design, all medical students at the University of Lausanne (Switzerland) were invited to complete four waves of yearly online questionnaires. Cognitive, affective, and behavioral empathy were measured with three validated instruments, and emotion recognition was assessed with a performance test. For each measure, linear mixed models including an array of psychosocial and health-related potential covariates were modelled. Different temporal variance-covariance structures and nonlinear trajectories were tested.
Results. The final sample included 3224 questionnaires completed by 1667 medical students. The cognitive and affective dimensions of empathy significantly increased in the first half of medical school, followed by a plateau, whereas behavioral empathy remained stable. For emotion recognition, a significant linear increase was observed. The only covariate with substantial influence was gender: students identifying as male presented similar trajectories of empathy and emotion recognition but with overall lower scores than students identifying as female or nonbinary.
Conclusions. This study revealed significant increases in cognitive empathy, affective empathy, and emotion recognition. Developments in today’s medical curricula may have contributed to the observed increase in empathy. Future multisite studies are warranted to identify the features of the educational environment that impact the trajectories of empathy during medical school.
Role of pharmacists in providing information on cancer genome medicine and patient decision making：open-label randomized controlled study
Authors: Saigo Orie , Nojiri Shuko , Asakura Kota , Koshiba Shoji , Saito Uki , Iwakawa Satoshi , Kimura Toshimi , Kato Shunsuke 
Published: October 2024

Abstract

Background
Cancer genome medicine based on genetic mutations is developing globally, and comprehensive genome profiling (CGP) are now routinely performed in Japan. Patient expectations are high for new treatment modalities based on genetic testing, but these are mostly unknown in Japan. The role of pharmacists in cancer genome medicine has not yet been established, but they can be a potential source of reliable information on cancer genome medicine, thus improving the health literacy of patients and supporting their decision making.
Methods
This was an open-label randomized controlled study. Subjects were randomly assigned 1:1 to the intervention and non-intervention groups, with treatment objective (preoperative and postoperative/advanced recurrence) as a stratification factor. In the non-intervention group, the pharmacist provided explanations about the drugs as done in usual care. In addition, the intervention group was provided with information on CGP. The primary endpoint was the impact of the pharmacist’s intervention on the patient’s decision making, measured by the Decisional Conflict Scale (DCS). As a secondary endpoint, factors influencing patient’s decision making were also analyzed.
Results
The analysis included responses from 180 patients. The pharmacist interview lasted 19.9 ± 6.9 and 10.4 ± 4.3 minutes for the intervention and non-intervention groups, respectively. The percentage of patients whose DCS scores decreased before and after the pharmacist interview was not significant between the intervention versus non-intervention groups (49.4% vs. 41.9%; p = 0.313, test of proportions). However, the DCS score of the intervention and non-intervention groups increased by 1.98 ± 16.5 and 3.61 ± 17.3, respectively. Both groups exhibited an increase in decisional conflict about receiving treatment, including CGP, but the change was significantly smaller in the intervention group (p = 0.026, unpaired t-test). Factors that influenced patient’s decision making through pharmacist intervention included age, chemotherapy being the primary treatment and having no history of nonsurgical treatment, such as chemotherapy, radiofrequency ablation, transcatheter arterial chemoembolization, and microwave ablation.
Conclusions
Increased conflicts is reduced when pharmacists provide information about CGP to patients undergoing cancer chemotherapy. This can help patients make informed decisions about their treatment choices.