bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete genome of Arthrobacter alpinus strain R3.8, bioremediation potential unraveled with genomic analysis
Authors: See-Too Wah-Seng , Ee Robson , Lim Yan-Lue , Convey Peter , Pearce David A. , Mohidin Taznim Begam Mohd , Yin Wai-Fong , Chan Kok Gan 
Published: September 2017

AbstractArthrobacter alpinus R3.8 is a psychrotolerant bacterial strain isolated from a soil sample obtained at Rothera Point, Adelaide Island, close to the Antarctic Peninsula. Strain R3.8 was sequenced in order to help discover potential cold active enzymes with biotechnological applications. Genome analysis identified various cold adaptation genes including some coding for anti-freeze proteins and cold-shock proteins, genes involved in bioremediation of xenobiotic compounds including naphthalene, and genes with chitinolytic and N-acetylglucosamine utilization properties and also plant-growth-influencing properties. In this genome report, we present a complete genome sequence of A. alpinus strain R3.8 and its annotation data, which will facilitate exploitation of potential novel cold-active enzymes.
Complete genome sequence of the sulfur-oxidizing chemolithoautotrophic Sulfurovum lithotrophicum 42BKTT
Authors: Jeon Wooyoung , Priscilla Lia , Park Gyuyeon , Lee Heeseok , Lee Narae , Lee Dongyup , Kwon Hyuksung , Ahn Iksung , Lee Changha , Lee Hongweon , Ahn Jungoh 
Published: September 2017

AbstractA sulfur-oxidizing chemolithoautotrophic bacterium, Sulfurovum lithotrophicum 42BKTT, isolated from hydrothermal sediments in Okinawa, Japan, has been used industrially for CO2 bio-mitigation owing to its ability to convert CO2 into C5H8NO4− at a high rate of specific mitigation (0.42 g CO2/cell/h). The genome of S. lithotrophicum 42BKTT comprised of a single chromosome of 2217,891 bp with 2217 genes, including 2146 protein-coding genes and 54 RNA genes. Here, we present its complete genome-sequence information, including information about the genes encoding enzymes involved in CO2 fixation and sulfur oxidation.
Treatment of chronic telogen effluvium with oral minoxidil: A retrospective study
Authors: Perera Eshini , Sinclair Rodney 
Published: September 2017

Background: Chronic telogen effluvium (CTE) may be primary or secondary to various causes, including drug reaction, nutritional deficiency and female pattern hair loss (FPHL). Oral minoxidil stimulates hair growth, and topical minoxidil is used in the treatment of FPHL and male androgenetic alopecia. minoxidil has not been used to treat CTE. This study aimed to assess the treatment of CTE with once daily oral minoxidil. Methods: Women with a diagnosis of CTE based on >6 month history of increased telogen hair shedding, no visible mid frontal scalp hair loss (Sinclair stage 1) and no hair follicle miniaturization on scalp biopsy were treated with once daily oral minoxidil. Hair shedding scores (HSS) at baseline, 6 and 12 months were analysed using the Wilcoxon rank sum test for pair-wise comparisons. Results: Thirty-six women were treated with oral minoxidil (range, 0.25-2.5 mg) daily for 6 months. Mean age was 46.9 years (range 20-83), HSS at baseline was 5.64, and duration of diagnosis was 6.55 years (range 1-27). There was a reduction in mean HSS scores from baseline to 6 months of 1.7 (p<0.001) and baseline to 12 months of 2.58 (p<0.001). Five women who described trichodynia at baseline, noted improvement or resolution within 3 months. Mean change in blood pressure was minus 0.5 mmHg systolic and plus 2.1 mmHg diastolic. Two patients developed transient postural dizziness that resolved with continued treatment. One patient developed ankle oedema. Thirteen women developed facial hypertrichosis. For 6 patients this was mild and did not require treatment; 4 had waxing of their upper lip or forehead; 3 had laser hair removal. No patients developed any haematological abnormality. All 36 women completed 12 months of treatment. Conclusions: Once daily oral minoxidil appears to reduce hair shedding in CTE. Placebo controlled studies are recommended to further assess this response.
Managerial Incentives to Increase Risk Provided by Debt, Stock, and Options
Authors: Anderson Joshua D. , Core John E. 
Published: September 2017

We measure a manager’s risk-taking incentives as the total sensitivity of the manager’s debt, stock, and option holdings to firm volatility. We compare this measure with the option vega and with the relative measures used by the prior literature. Vega does not capture risk-taking incentives from managers’ stock and debt holdings and does not reflect the fact that employee options are warrants. The relative measures do not incorporate the sensitivity of options to volatility. Our new measure explains risk choices better than vega and the relative measures and should be useful for future research on managers’ risk choices. Data and the online appendix are available at https://doi.org/10.1287/mnsc.2017.2811 . This paper was accepted by Shiva Rajgopal, accounting.
Disruption as a communicative strategy: The case of #FeesMustFall and #RhodesMustFall students’ protests in South Africa
Authors: Mpofu Shepherd 
Published: September 2017

Abstract
In 1994 South Africa became a miracle in the world of postcolonies as a newly independent ‘rainbow’ nation state. Apartheid was replaced by an informal but still identical system which I refer to as apartheid. Good governance, democracy, peace, civility and quiet are framed by the media and regarded by investors and political elite among others to be the preferred set-up of things. Using the rage in the #RhodesMustFall and #FeesMustFall student protests as data, I argue that disrupting the world as we know it in order to address the poor’s grievances is part and parcel of strategic and effective communication especially for the marginalized poor majority black people whose dreams remain deferred. This argument will be framed by questions around the current burdens of apartheid, the achievements of disruptive protests and the meaning, roles and behaviours of officialdom towards members and ideologies of Fallist movements.
Hölder gradient estimates for a class of singular or degenerate parabolic equations
Authors: Imbert Cyril , Jin Tianling , Silvestre Luis 
Published: September 2017

Abstract
We prove interior Hölder estimates for the spatial gradients of the
viscosity solutions to the singular or degenerate parabolic equation

u_{t}=\lvert\nabla u\rvert^{\kappa}\operatorname{div}(\lvert\nabla u\rvert^{p-%
2}\nabla u),

where

{p\in(1,\infty)}

and

{\kappa\in(1-p,\infty)}

. This includes
the from

{L^{\infty}}

to

{C^{1,\alpha}}

regularity for parabolic
p-Laplacian equations in both divergence form with

{\kappa=0}

, and
non-divergence form with

{\kappa=2-p}

.
From Shame to Shaming: towards an Analysis of Shame Narratives
Authors: Kasabova Anita 
Published: September 2017

Abstract
This paper examines shame in three narratives involving the social self and the evaluative perception of that self. The semiotic square is used for analysing some of the conceptualizations of shame and highlighting the structural relations of the shame phenomenon in narrative. The stance of this paper is that shame is a moral emotion which results from two main evaluative perceptions, involving moral sentiment and social reputation. In support of this claim, different aspects of shame are analysed in three types of narratives from different socio-cultural contexts.
THz plasmonic resonances in hybrid reduced-graphene-oxide and graphene patterns for sensing applications
Authors: Mencarelli Davide , Nishina Yuta , Ishikawa Atsushi , Pierantoni Luca , Bellucci Stefano 
Published: September 2017

AbstractIn this contribution, we effectively combine important developments of nowadays technology: graphene based THz plasmonics, reduced-graphene-oxide (rGO) based sensors, and capability of patterning graphene materials at micro and nano scale. Surface waves in graphene were observed for the first time only few years ago, confirming the ability of this exceptional material to support plasmons at relatively low frequencies - a few THz - due to its intrinsically huge selfinductance. On the other hand, graphene oxide, and its reduced forms, has emerged as a very interesting material for several applications, including gas sensors and biosensors. In this work, the possibility of a _ne and controlled patterning of the above materials is considered as a useful degree of freedom to govern plasmon resonances and consequent electromagnetic absorption. In particular, the excitation of THz plasmons in arrays of nanoribbons, made of graphene and rGO, has been deeply investigated, in order to quantify the sensitivity to surface changes of conductivity, due to possible external perturbations. Fullwave analysis of hybrid metal-graphene-rGO is also presented and discussed. The effects of substrate thickness and of higher diffraction modes are rigorously taken into account, as a possible mean to enhance the sensing capability of the proposed device.
Glycinergic Input to the Mouse Basal Forebrain Cholinergic Neurons
Authors: Bardóczi Zsuzsanna , Pál Balázs , Kőszeghy Áron , Wilheim Tamás , Watanabe Masahiko , Záborszky László , Liposits Zsolt , Kalló Imre 
Published: September 2017

The basal forebrain (BF) receives afferents from brainstem ascending pathways, which has been implicated first by Moruzzi and Magoun (1949) to induce forebrain activation and cortical arousal/waking behavior; however, it is very little known about how brainstem inhibitory inputs affect cholinergic functions. In the current study, glycine, a major inhibitory neurotransmitter of brainstem neurons, and gliotransmitter of local glial cells, was tested for potential interaction with BF cholinergic (BFC) neurons in male mice. In the BF, glycine receptor α subunit-immunoreactive (IR) sites were localized in choline acetyltransferase (ChAT)-IR neurons. The effect of glycine on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous IPSCs (sIPSCs; 0.81 ± 0.25 × 10−1Hz) recorded in whole-cell conditions. Potential neuronal as well as glial sources of glycine were indicated in the extracellular space of cholinergic neurons by glycine transporter type 1 (GLYT1)- and GLYT2-IR processes found in apposition to ChAT-IR cells. Ultrastructural analyses identified synapses of GLYT2-positive axon terminals on ChAT-IR neurons, as well as GLYT1-positive astroglial processes, which were localized in the vicinity of synapses of ChAT-IR neurons. The brainstem raphe magnus was determined to be a major source of glycinergic axons traced retrogradely from the BF. Our results indicate a direct effect of glycine on BFC neurons. Furthermore, the presence of high levels of plasma membrane glycine transporters in the vicinity of cholinergic neurons suggests a tight control of extracellular glycine in the BF.SIGNIFICANCE STATEMENTBasal forebrain cholinergic (BFC) neurons receive various activating inputs from specific brainstem areas and channel this information to the cortex via multiple projections. So far, very little is known about inhibitory brainstem afferents to the BF. The current study established glycine as a major regulator of BFC neurons by (1) identifying glycinergic neurons in the brainstem projecting to the BF, (2) showing glycine receptor α subunit-immunoreactive (IR) sites in choline acetyltransferase (ChAT)-IR neurons, (3) demonstrating glycine transporter type 2 (GLYT2)-positive axon terminals synapsing on ChAT-IR neurons, and (4) localizing GLYT1-positive astroglial processes in the vicinity of synapses of ChAT-IR neurons. The effect of glycine on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous IPSCs recorded in whole-cell conditions.
Pertumbuhan Stek Krisan (Chrysanthemum morifolium (L.) Ramat) pada Berbagai Media Kultur In Vitro
Authors: Kristianti Adven , Kamsinah Kamsinah , Dwiati Murni 
Published: September 2017

Chrysanthemum (Chrysanthemum morifolium (L.) Ramat is one of ornamental plant commodities with high aesthetic value. As a consequence of increasing demand, the availability of seeds in adequate number is needed with method in vitro. This study aims to find out the effect of various in vitro culture media on the growth of chrysanthemum explants and to determine alternative media other than MS for in vitro growth of chrysanthemum explants. Experimental method arranged in a Randomized Complete Block Design (RCBD) with four treatments and four blocks is applied. The treatments are Murashige and Skoog (MS), Vacint and Went (V&W), Knudson and modified media, while node position serves as block. Time of shoot emergence, shoot length, number of leaves on each shoot, and number of roots are measured. Data are analyzed using Analysis of Variance (F test) with confidence intervals of 95% and 99%. Least Significant Difference (LSD) test is applied when significant difference among variables is observed. Culture media shows significant effect on chrysanthemum explant in vitro growth. Knudson media is found as an alternative media besides MS sufficiently suitable to increase chrysanthemum explants in vitro growth.
Deteksi Resistensi Amoxicillin Helicobacter pylori pada Pasien Dispepsia
Authors: Sulistiyawati Indah , Arinton I Gede , Pramono Hendro 
Published: September 2017

Amoxicillin is one of the antibiotics that commonly used on treatment of H. pylori infection. pbp1A gene mutation in H. pylori is a dominant cause of amoxicillin resistance. This study was aimed to evaluate the presence of H. pylori in patients with dyspepsia by using non-invasive method i.e. H. pylori stool antigen (HPSA) and invasive method i.e. pbp1A gene amplification, and also evaluate the amoxicillin resistance of H. pylori by assessing the pbp1A gene mutations. The samples were 26 faeces and 26 gastric biopsies of patients with dyspepsia from the Internal Disease of Prof. Dr. Margono Soekardjo Hospital in Purwokerto. DNA amplification performed by using polymerase chain reaction (PCR) to detect the presence of amoxicillin resistance encoding gene i.e. penicillin binding protein (pbp1A). Sequencing of the DNA sample was performed at the First Base Malaysian Company, to analyze the existence of a point mutation. DNA sequencing analysis of 12 samples showed the presence of a mutations in pbp1A gene from 2 samples, in the third motive of pbp i.e. amino acid changes, Alanine 599 substituted to Threonin and Threonin 592 to Alanine. Those mutations become a dominant risk factor for resistance of H. pylori, toward the bacterial peptidoglycan synthesis. In this research, it was known that the detection of H. pylori infection by using PCR remains more accurate and specific method. The presence of H. pylori mutant strains in this study may becomes the risk factors of resistance to amoxicillin treatment.
Karakteristik Morfologi Mycobacterium tuberculosis yang Terpapar Obat Anti TB Isoniazid (INH) secara Morfologi
Authors: Widodo Widodo , Irianto Agus , Pramono Hendro 
Published: September 2017

INH mempunyai peranan dalam menghambat sintesis asam mikolat yang merupakan komponen pembentuk dinding sel M. tuberculosis. Mengetahui pengaruh pemaparan obat INH terhadap karakteristik M. tuberculosis secara morfologi sel. Metode penelitian eksperimen dengan menggunakan Rancangan percobaan acak Lengkap dengan variasi waktu pemaparan 24 jam, 48 jam, 72 jam dan 96 jam pada konsentrasi INH 0,5 μg/ml dengan jumlah ulangan 6. Hasil penelitian menunjukkan ada pengaruh pada masing-masing perlakuan INH 0,5 μg/ml terhadap karakteristik M. tuberculosis, pada pemaparan 24 jam diperoleh persentase pertumbuhan 18,25%, ukuran rerata sel 1,64 x 0,35 µm, persentase kerusakan sel 28,1%. Pada pemaparan 48 jam diperoleh persentase pertumbuhan sel 7,9%, ukuran rerata sel 1,64 x 0,36 µm, persentase kerusakan sel 41%, pada pemaparan 72 jam diperoleh persentase pertumbuhan sel 7,0%, ukuran rerata sel 1,64 x 0,32 µm, persentase kerusakan sel 53,4%, pada pemaparan 96 jam diperoleh persentase pertumbuhan sel 1,6%, ukuran rerata sel 1,61 x 0,29 µm, persentase kerusakan sel 57%. Perubahan morfologi sel yang terpapar INH 0,5 μg/ml pada permukaan sel terjadi kerutan dan penyusutan panjang sel setelah regenerasi, permukaan sel mulai kasar dan sedikit ada kerusakan, sel mengalami lisis dan sebagian sel berbentuk oval , sel mengalami perubahan bentuk menjadi kokus. karakteristik M. tuberculosis yang terpapar INH 0,5 µg/ml mengalami perubahan morfologi sel, menjadi lebih pendek dan perubahan bentuk dari batang menjadi kokus, namun perubahan morfologi ini tidak terjadi secara permanen pada generasi berikutnya.Kata kunci : M. tuberculosis, INH, Morfologi,
Perkembangan Awal Protocorm Anggrek Phalaenopsis amabilis secara In Vitro setelah Penambahan Zat Pengatur Tumbuh α-Naphtaleneacetic Acid dan Thidiazuron
Authors: Ningrum Eka Fitriana Candra , Rosyidi Ikhsanudin Nur , Puspasari Rizka Riliant , Semiarti Endang 
Published: September 2017

Phalaenopsis amabilis (L.) Blume merupakan salah satu anggrek alam dengan nilai komersial yang tinggi. Keberadaannya di alam semakin sukar dijumpai akibat eksploitasi berlebihan dan kerusakan hutan. Selain itu budidaya anggrek ini cukup sulit dilakukan karena biji bersifat mikroskopis dan tidak memiliki endosperm, sehingga perlu dilakukan kultur in vitro. Dalam budidaya secara in vitro diperlukan medium pendukung pertumbuhan anggrek secara optimal misalnya dengan penambahan Zat Pengatur Tumbuh (ZPT) dari golongan auksin dan sitokinin. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan ZPT tersebut terhadap perkembangan protokorm (pertumbuhan embrio anggrek) P. amabilis secara in vitro dari aspek morfologi dan anatomi. Penelitian dilakukan di Laboratorium Bioteknologi dan Laboratorium Struktur Perkembangan Tumbuhan. Digunakan protokorm anggrek P. amabilis berumur 2 bulan. Protokorm disubkultur pada medium NP dengan kombinasi ZPT NAA dan TDZ pada berbagai konsentrasi dan diinkubasi pada ruangan dengan kelembaban tinggi, suhu 250C. Kemudian diamati protokorm anggrek selama 2 minggu yang mencakup persentase pertumbuhan, persentase protokorm yang membentuk tunas dan absorbing hair. Pembuatan preparat anatomi dilakukan dengan mengambil protokorm setiap minggunya, disimpan dalam larutan alkohol 70% dan dibuat preparat anatomi menggunakan metode Parafin dengan pewarnaan safranin. Hasil penelitian menunjukkan pada penambahan NAA dan TDZ pertumbuhan protokorm mencapai 100%. Persentase protokorm yang membentuk tunas berturut turut (0:0, 0.2:0, 0:0.5, 0.2:0.5, 0:1, dan 0.2:1) ppm adalah 24%, 22%, 32%, 40%, 40% dan 38% sedangkan untuk pengamatan protokorm yang membentuk absorbing hair sebesar 10%, 18%, 14%, 24%, 16%, dan 0%. Untuk diameter sel berukuran 39.2±0.47µm, 44.4±0.97µm, 39.08±0.5µm, 38.83±0.2µm,39.5±0.39µm dan 39.75±0.28µm. Sehingga dapat disimpulkan medium yang paling optimal dalam pertumbuhan anggrek P. amabilis secara in vitro adalah medium NP dengan kombinasi NAA dan TDZ (0.2 : 0.5) ppm.Kata kunci : Anatomi, Kultur In Vitro, Morfologi, Phalaenopsis amabilis
The Influence of Arthritis in Other Major Joints and the Spine on the One-Year Outcome of Total Hip Replacement
Authors: Huber Joerg , Dieppe Paul , Dreinhoefer Karsten , Günther Klaus-Peter , Judge Andrew 
Published: September 2017

Background:
Although arthritis in other affected major joints and back pain are known to lead to worse outcomes following total hip replacement, to our knowledge, these risk factors have not previously been operationalized as a musculoskeletal morbidity profile. The aim of this study was to measure the influence of other major joints and the spine (as grades of musculoskeletal morbidity) on the 1-year outcome of primary total hip replacement.

Methods:
The EUROHIP study consists of 1,327 patients undergoing primary total hip replacement for arthritis across 20 European orthopaedic centers. The primary outcome was the responder rate at 12 months calculated with the relative effect per patient for total hip replacement using the total Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score. The primary predictor of interest was different combinations of arthritis of major joints and the spine grouped into 4 musculoskeletal morbidity grades: 1 (single major joint), 2 (multiple major joints), 3 (single major joint and spine), and 4 (multiple major joints and spine). The confounders adjusted for were age, sex, body mass index, living situation, years of hip pain, American Society of Anesthesiologists (ASA) class, anxiety or depression, and preoperative WOMAC subscales.

Results:
For this analysis, 845 patients were included with complete 12-month follow-up WOMAC scores. The mean patient age was 65.7 years, and 55.2% of patients were female.

Conclusions:
The involvement of other major joints and the spine assessed as 1 of 4 musculoskeletal morbidity grades had a strong influence on the 1-year outcome after total hip replacement. The effect size was large compared with other risk factors. Even so, the majority of patients in musculoskeletal morbidity grade 4 had favorable outcomes from the surgical procedure (>74% response to surgical procedures).

Level of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
A Refueling Scheme Optimization Model for the Voyage Charter with Fuel Price Fluctuation and Ship Deployment Consideration
Authors: Jia Peng , Zhang Weilun , Wenhao E , Sun Xueshan 
Published: September 2017

Abstract
Due to the long operation cycle of maritime transportation and frequent fluctuations of the bunker fuel price, the refueling expenditure of a chartered ship at different time or ports of call make significant difference. From the perspective of shipping company, an optimal set of refueling schemes for a ship fleet operating on different voyage charter routes is an important decision. To address this issue, this paper presents an approach to optimize the refueling scheme and the ship deployment simultaneously with considering the trend of fuel price fluctuations. Firstly, an ARMA model is applied to forecast a time serials of the fuel prices. Then a mixed-integer nonlinear programming model is proposed to maximize total operating profit of the shipping company. Finally, a case study on a charter company with three bulk carriers and three voyage charter routes is conducted. The results show that the optimal solution saves the cost of 437,900 USD compared with the traditional refueling scheme, and verify the rationality and validity of the model.