bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prophylaxis of respiratory syncytial virus infection: current status and prospects for vaccine development
Authors: Korovkin A. S. , Gorenkov D. V. , Volgin A. R. 
Published: October 2024

INTRODUCTION. Respiratory syncytial virus (RSV) is one of the most widespread pathogens that typically cause acute upper and lower respiratory tract infections in children and adults. Monoclonal antibody products have long been used for passive immunoprophylaxis in premature infants with bronchopulmonary dysplasia. However, vaccines have recently been licensed for the prophylactic immunisation of older adults with various risk factors for severe RSV infection (primarily, chronic cardiovascular and respiratory diseases and diabetes mellitus).AIM. This study aimed to review the current status of the development of vaccines for active immunisation against RSV infection, including the epidemiological rationale for their development, clinical trial results for licensed vaccines, recommendations for vaccination, and promising pipeline vaccines for RSV prevention.DISCUSSION. Initially hindered by the unique immunopathogenesis of RSV infection and the genetic hypervariability of RSV for a long time, attempts to develop an RSV vaccine succeeded when international researchers managed to identify a conservative neutralising antibody target capable of inducing specific immunity against RSV. This target, the RSV capsid protein stabilised in its prefusion (pre-F) conformation, can mediate viral entry into the cell following a conformational change. Several subunit vaccines based on recombinant pre-F proteins have successfully passed clinical trials and have been approved for the immunisation of older adults. In addition, one of these vaccines has been recommended for use during pregnancy to prevent RSV infection in newborns and infants. Currently, programmes are being implemented to develop novel RSV vaccines based on messenger RNA (mRNA) and non-replicating attenuated influenza vectors. This article examines and summarises the efficacy and safety parameters of two RSV vaccines approved in multiple countries around the world. Both vaccines have satisfactory safety profiles and comparable prophylactic efficacy parameters.CONCLUSIONS. Prelicensure clinical trials of novel RSV vaccines, including those being developed in the Russian Federation, should include prophylactic efficacy assessments in target patient populations. For vaccines approved by leading international regulators, clinical trials required for approval in the Russian Federation will be limited to bridging studies confirming the immunogenicity and safety of the vaccines.
Molecular genetic methods for quality control of inactivated vaccines using a Chikungunya virus model: vaccine strain identification and completeness of virus inactivation
Authors: Oksanich A. S. , Samartseva T. G. , Kaa K. V. , Otrashevskaia E. V. , Krasko A. G. , Laputina A. G. , Netesova N. A. , Ignatyev G. M. 
Published: October 2024

INTRODUCTION. The completeness of virus inactivation and the identity of the vaccine strain are essential parameters for the safety and quality of inactivated virus vaccines, which should be controlled during vaccine development and production. Currently, the most promising quality control methods for inactivated virus vaccines are molecular genetic methods, which provide rapid results with high sensitivity and specificity.AIM. The aim of this study was the development of a real-time quantitative polymerase chain reaction (qPCR) method and an integrated cell culture real-time quantitative polymerase chain reaction (ICC-qPCR) method to assess the completeness of virus inactivation, as well as a reverse-transcription polymerase chain reaction assay coupled with restriction fragment length polymorphism analysis (RT-PCR-RFLP) to confirm the identity of the vaccine virus strain.MATERIALS AND METHODS. This study used RNA of CHIKV genotypes (three strains of each of the four CHIKV genotypes, including Asian, West African (WAf), and East/Central/South African (ECSA) genotypes, and the Indian Ocean Lineage of the ECSA genotype (ECSA-IOL), which were identified by sequencing prior to analysis). Additionally, the study used the Nika21 CHIKV strain (ECSA genotype), the Nika21 CHIKV strain inactivated with β-propiolactone, and the Nika21 CHIKV strain antigen adsorbed on aluminium hydroxide. The methods used included real-time qPCR, RT-PCR-RFLP, and virus neutralisation.RESULTS. The study identified a 218 bp fragment of the nsP1 gene (positions 789 to 1006) with restriction endonuclease recognition sites. These sites were present or absent in combinations specific to each of the four CHIKV genotypes. The authors selected primers for amplification of the specified gene region and tested the conditions for real-time qPCR and RT-PCR-RFLP. The study demonstrated the possibility of using the ICC-qPCR method to confirm the completeness of virus inactivation and the RT-PCR-RFLP method to identify the vaccine strain.CONCLUSIONS. The study showed the advantages of using the ICC-qPCR method to confirm the completeness of antigen inactivation and the RT-PCR-RFLP method to identify the vaccine strain. These methods are more sensitive and faster than traditional culture methods. ICC-qPCR and RT-PCR-RFLP can be used at any stage of the production process for inactivated vaccines.
Current regulatory requirements for stability studies of biological medicinal products: a review
Authors: Soldatov A. A. , Yakovlev A. K. , Avdeeva Zh. I. , Gorenkov D. V. , Korovkin A. S. , Kosenko V. V. 
Published: October 2024

INTRODUCTION. The stability assessment of biological medicinal products (BMPs) requires special approaches and regulatory requirements. Therefore, BMPs require relevant national guidelines, which are an important prerequisite for the assurance of the safe and effective use of BMPs.AIM. This study aimed to analyse national and international requirements for the stability assessment of BMPs in order to use the results to inform future development of a unified regulatory approach to estimating and confirming shelf-life periods for BMPs.DISCUSSION. Most biotechnological medicinal products (BTMPs) are proteins and are highly sensitive to environmental factors by nature. Therefore, the shelf life of a BTMP is established on the basis of real-time stability studies. Stability testing under accelerated and stress conditions is conducted to support shelf-life claims and to characterise the mechanism of protein structure degradation. The results of accelerated and stress studies can be used to select the most sensitive stability-indicating parameters and testing methods. National and international regulatory authorities have developed specialised guidelines for stability studies of BMPs of various origins, and the stability assessment approaches in the regulatory system of the Eurasian Economic Union (EAEU) are harmonised with international standards. Special considerations associated with the stability of vaccines imply that stability studies of vaccines should not only establish shelf life but also investigate stability after reconstitution and after short-term temperature excursions from the recommended cold-chain conditions.CONCLUSIONS. Special stability testing considerations for various groups of BMPs (including BTMPs and immunobiologicals) indicate the need to develop and improve the system of requirements for BMP stability assessment. This will facilitate the optimisation of the life cycle of BMPs in the Russian Federation and the other EAEU member states.
ANALISIS TRANSFER DATA PADA JARINGAN TERDAMPAK ARP SPOOFING MENGGUNAKAN METODE ARP POISONING DAN STATISTIK DESKRIPTIF
Authors: Sudaryanto  , Dwi Nugraheny  
Published: October 2024

This Computer network security issues are very important and need to be considered in the development of computer networks. Networks connected to network devices are usually vulnerable to hacking. Hacking is an activity that allows a person or group to change or take data for personal gain. The aim of this research is to carry out testing and analysis to determine the condition and measure the level of security of the ITDA Yogyakarta intra-campus information system and computer network. Describe security gaps and measure the level of security that needs to be immediately repaired so that it can help correct failures in maintaining the security of ITDA Yogayakarta intra-campus information systems and networks. This research uses descriptive statistics with 20 PC units as samples. There were four tests in this study with a total success of 16 out of 20 samples. From the results of Arp spoofing on the local network, it can be concluded that after the local network is infiltrated by an attacker using the ARP spoofing method, the target traffic will be redirected to the attacker's device. This can allow attackers to monitor and understand the contents of data traffic on the local network. Changing the attacker's MAC address is very necessary because if the MAC is not replaced then network traffic will not be redirected to the attacker's device.
Meningkatkan Regulasi Emosi dengan Mengurangi Deindividuasi pada Gen Z dalam Mencegah Perilaku Hate Speech pada Media Sosial
Authors: Rahma Dwi Nur , Matulessy Andik , Pratitis Nindia 
Published: October 2024

Social media is a tool that allows someone to interact online without being limited by space and time. One of them is the social media Instagram, whose existence is often misused by irresponsible users to behave in hate speech. Group situations allow a person's self-awareness to be reduced and with low emotional regulation it allows a person to behave hate speech. This study aims to determine the relationship between deindividuation and emotion regulation with hate speech behavior in Generation Z who use social media. The method used in this research is quantitative correlation with a total of 115 Generation Z subjects aged 18-28 years. The collection of subjects using incidental sampling technique. The results of data analysis using Spearman's Rho Non-Parametric Correlation produced a significant positive relationship between Deindividuation and Hate Speech behavior in Generation Z of social media users and there was a significant negative relationship between Emotion Regulation and Hate Speech Behavior in Generation Z of social media users. The existence of a positive relationship means that the higher the deindividuation in Generation Z, the higher the Hate Speech behavior and vice versa. The existence of a negative relationship means that the lower the Emotion Regulation in Generation Z, the higher Hate Speech behavior will be and vice versa.

Media sosial merupakan sarana yang memungkinkan seseorang dapat berhubungan secara online tanpa dibatasi ruang dan waktu. Salah satunya adalah media sosial instagram, keberadaannya sering disalahgunakan pengguna yang tidak bertanggung jawab untuk berperilaku hate speech. Situasi kelompok memungkinkan kesadaran diri seseorang akan berkurang dan dengan regulasi emosi yang rendah maka memungkinkan seseorang untuk berperilaku hate speech. Penelitian ini bertujuan untuk mengetahui hubungan antara deindividuasi dan regulasi emosi dengan perilaku hate speech pada Generasi Z pengguna media sosial. Metode yang digunakan dalam penelitian ini adalah kuantitatif korelasional dengan jumlah subjek sebanyak 115 Generasi Z yang berusia 18-28 tahun. Pengumpulan subjek menggunakan teknik insidental sampling. Hasil analisis data menggunakan Korelasi Non-parametrik Spearman’s Rho menghasilkan hubungan positif yang signifikan antara Deindividuasi dengan perilaku Hate Speech pada Generasi Z pengguna media sosial serta terdapat hubungan negatif yang signifikan antara Regulasi Emosi dengan Perilaku Hate Speech pada Generasi Z pengguna media sosial. Adanya hubungan positif dapat diartikan bahwa semakin tinggi deindividuasi pada Generasi Z maka perilaku Hate Speech juga semakin tinggi dan sebaliknya. Adanya hubungan negatif dapat diartikan bahwa semakin rendah Regulasi Emosi pada Generasi Z maka perilaku Hate Speech akan semakin tinggi dan begitu juga sebaliknya.
Homesickness pada mahasiswa rantau tahun pertama: Apakah berhubungan dengan cultural intelligence dan happiness?
Authors: Nisa Mahfudhotin Nur Khoirotun , Santi Dyan Evita , Ananta Aliffia 
Published: October 2024

This research aimed to determine the relationship between cultural intelligence and happiness with homesickness in first year overseas students. Homesickness refers to feelings of suffering caused by the loss of leaving home. Homesickness is characterized by feelings of loneliness, discomfort and difficulty adjusting to a new environment. This study used 101 subjects who were students on campus Sukolilo city of Surabaya class of the first year. Data collection in this study used a Likert scale, using measurement instruments consisting of a homesickness questionnaire (HQ), cultural intelligence scale and happiness scale. Simultans Bayesian Regression Analysis shows that there is a relationship between cultural intelligence and happiness on homesickness. Partially, cultural intelligence and happiness can be significant predictors of homesickness. That is, the higher the cultural intelligence and happiness, the lower the homesickness of a first year overseas student.

Penelitian ini bertujuan untuk mengetahui hubungan antara cultural intelligence dan happiness dengan homesickness pada mahasiswa rantau tahun pertama. Homesickness merujuk pada perasaan menderita yang disebabkan oleh perasaan kehilangan karena meninggalkan rumah. Homesickness ditandai dengan munculnya rasa kesepian, ketidaknyamanan, dan sulit untuk melakukan penyesuaian diri di lingkungan yang baru. Penelitian ini menggunakan 101 subjek yang merupakan mahasiswa yang berada di kampus kecamatan Sukolilo kota Surabaya angkatan tahun pertama. Pengumpulan data pada penelitian ini menggunakan skala likert, dengan menggunakan instrumen pengukuran yang terdiri dari homesickness questionnaire (HQ), cultural intelligence scale, dan happiness scale. Analisis Regresi Bayesian secara simultan menunjukkan bahwa terdapat hubungan antara cultural intelligence dan happiness terhadap homesickness. Secara parsial, cultural intelligence dan happiness dapat menjadi prediktor yang signifikan terhadap homesickness. Artinya, semakin tinggi cultural intelligence dan happiness maka semakin rendah homesickness yang dimiliki mahasiswa rantau tahun pertama.
Self-Efficacy dan Dukungan Sosial dengan Quarter Life Crisis pada Karyawan
Authors: Sari Sintikhe Kurnia , Rini Amanda Pasca , Ariyanto Eko April 
Published: October 2024

Each individual will be faced with personal questions such as jobs, salaries, positions and so on. Situations like these can affect individuals. Comparing other people's achievements, worrying about the future and feeling worried. If this happens at the age of 20-30 years it is called a quarter life crisis. This study aims to determine the relationship between self-efficacy and social support with quarter life crisis among employees at PT.X. This research is a quantitative study with a population of 277 employees using simple random sampling techniques so that this study involved 164 employees aged 20-30 years. Data collection uses 3 scales, namely the self-efficacy scale, social support scale and quarter life crisis scale. The data were analyzed using SPSS version 16.0 for windows with multiple regression analysis techniques. The results of the analysis show that the value of self-efficacy has a negative correlation of -0.668 with sig. 0.000 (<0.05) and the value of social support has a negative correlation of -0.469 with sig. 0.000 (<0.05). This shows that the higher the value of self-efficacy and social support, the lower the value of quarter life crisis, and vice versa.

Setiap individu akan dihadapkan dengan pertanyaan-pertanyaan pribadi seperti pekerjaan, gaji, jabatan dan sebagainya. Keadaan seperti ini dapat mempengaruhi individu. Membandingkan pencapaian orang lain, cemas terhadap masa depan serta perasaan khawatir. Hal ini jika terjadi pada usia 20-30 tahun disebut dengan quarter life crisis. Penelitian ini bertujuan untuk mengetahui hubungan self-efficacy dan dukungan sosial dengan quarter life crisis pada kalangan karyawan di PT.X. Penelitian ini merupakan penelitian kuantitatif yang dengan populasi penelitian sebanyak 277 karyawan dengan teknik pengambilan simple random sampling sehingga penelitian ini melibatkan 164 karyawan dengan usia 20-30 tahun. Pengambilan data menggunakan 3 skala yaitu skala self-efficacy, skala dukungan sosial dan skala quarter life crisis. Data tersebut dianalisis menggunakan SPSS version 16.0 for windows dengan teknik analisis regresi berganda. Hasil analisis menunjukan nilai self-efficacy memiliki korelasi negatif sebesar -0.668 dengan sig. 0.000 (<0.05) dan nilai dukungan sosial memiliki korelasi negatif sebesar -0.469 dengan sig. 0.000 (<0.05). Hal ini menunjukan bahwa semakin tinggi nilai self-efficacy dan dukungan sosial maka semakin rendah nilai quarter life crisis, dan juga sebaliknya.
Self-Discrepancy pada Pengguna Roleplayer: Bagaimana Peranan Penerimaan Diri?
Authors: Putri Adelia Elsiana , Pratikto Herlan , Pratikto Herlan , Suhadianto Suhadianto 
Published: October 2024

Abstract
This research aims to determine whether there is a relationship between self-acceptance and self-discrepancy among roleplayer users. The research participants amounted to 170 individuals obtained through purposive sampling. This study utilizes two scales: the self-acceptance scale and the self-discrepancy scale. The data collection instrument in this research employs the Likert scale model. The sampling technique used in this study is correlational. Data analysis in this research employs the Pearson product-moment correlation method. Based on the data analysis results obtained from the SPSS version 16.0 (Statistical Product and Service Solution) for Windows, there is a significant negative correlation. This means that the higher the level of self-acceptance possessed by an individual, the lower the likelihood of that individual experiencing self-discrepancy. Conversely, if an individual has a lower level of self-acceptance, there is a higher likelihood of experiencing self-discrepancy.

Abstrak
Penelitian ini bertujuan untuk mengetahui apakah ada hubungan antara penerimaan diri dengan self-discrepancy pada pengguna roleplayer. Partisipan penelitian berjumlah 170 orang yang diperoleh melalui teknik purposive sampling. Penelitian ini menggunakan dua skala yaitu skala penerimaan diri dan skala self-discrepancy. Instrumen pengambilan data pada penelitian ini menggunakan model skala likert. Teknik sampel yang digunakan pada penelitian ini menggunakan teknik korelasional. Analisis data pada penelitian ini menggunakan metode korelasi pearson product moment. Berdasarkan hasil analisis data yang diperoleh dari program computer SPSS versi 16.0 (Statistical Product and Service Solution) for Windows terdapat hasil analisis data yang menunjukkan bahwa terdapat hubungan negatif yang sigifikan. Artinya semakin tinggi tingkat penerimaan diri yang dimiliki oleh individu maka akan semakin rendah individu tersebut akan mengalami self-discrepancy. Sebaliknya, apabila semakin rendah tingkat penerimaan diri yang dimiliki oleh individu maka akan semakin tinggi individu tersebut akan mengalami self-discrepancy.
Body Dissatisfaction Ibu Pasca Melahirkan : Bagaimana Peran Social Comparison?
Authors: Dinda WarapSari Dheasty Ferdhinia , Pratikto Herlan , Aristawati Akta Ririn 
Published: October 2024

Abstract
The body is the most important physical asset for every human being because it is the easiest shape to see. Having an ideal body shape is the dream of all women. Dissatisfaction with the body is often felt by most women in the community. When a woman has entered into the gates of marriage, of course they will not be long to feel the period of pregnancy and childbirth This study has the aim of knowing whether there is a relationship between social comparison with body dissatisfaction in postpartum mothers. This research is correlational research. The subjects in this study were 131. Hypothesis testing was carried out using Spearman Rho's test technique. The results of this study indicate a negative relationship between social comparison and body dissatisfaction in postpartum mothers.

Keywords : 1) Body Dissatisfaction; 2) Postpartum Mothers; 3) Social Comparisson

Abstrak
Tubuh merupakan aset fisik yang terpenting bagi setiap manusia dikarenakan bentuk yang paling mudah untuk dilihat. Memiliki bentuk tubuh yang ideal merupakan idaman semua wanita. Rasa ketidakpuasan yang terjadi pada tubuh kerap dirasakan oleh sebagian besar wanita yang berada dilingkungan masyarakat. Ketika seseorang wanita telah masukke dalam gerbang pernikahan, tentu saja mereka tidak akan lama untuk merasakan masa kehamilan dan melahirkan Penelitian ini mempunyai tujuan untuk mengetahui apakah ada hubungan antara social comparison dengan body dissatisfaction pada iibu pasca melahirkan. Penelitian ini adalah penelitian korelasional. Subjek dalam penelitian ini sebanyak 131. Pengujian hipotesis yang dilakukan menggunakan teknik uji spearman rho’s. Hasil penelitian ini menunjukkan adanya hubungan yang negatif antara social comparison dengan body disatisfaction pada ibu pasca melahirkan.

Kata kunci: 1) Body Dissatisfaction; 2) Ibu Pasca Melahirkan ;3) Social Comparisson
Kecanduan media sosial pada remaja: Bagaimana peran fear of missing out?
Authors: Maslukha Khoirum , Pratitis Niken Titi , Arifiana Isrida Yul 
Published: October 2024

Abstract
Many teenagers are addicted to social media because they have a desire to know everything that happens on social media, so they feel anxious if they miss a moment or information which causes someone to feel left behind and think that other people's lives on social media are more fun than their own life or often called the fear of missing out. This research aims to determine the relationship between fear of missing out and social media addiction in adolescents. The research instruments used in this study were the fear of missing out scale and the social media addiction scale. The sample in this research was students from SMP 17 August 1945 Surabaya. The sampling technique in this study used a quota sampling technique whose number was determined using the Krecjie table so that 162 subjects were obtained. The data analysis method used in this research uses Spearman Rho correlational analysis. The results of this study show that the hypothesis is accepted and there is a positive relationship between fear of missing out and social media addiction.
Keywords: fear of missing out, addiction to social media, teenagers.
Abstrak
Banyak remaja yang mengalami kecanduan media sosial dikarenakan memiliki keinginan untuk mengetahui segala sesuatu yang terjadi pada sosial media, sehingga memiliki rasa kecemasan jika kehilangan momen atau informasi yang menyebabkan seseorang merasa tertinggal dan berpikir bahwa kehidupan orang lain di media sosial lebih menyenangkan dibanding hidupnya sendiri atau sering disebut fear of missing out. Penelitian ini bertujuan untuk mengetahui hubungan antara fear of missing out dengan kecanduan media sosial pada remaja. Intrumen penelitian yang digunakan pada penelitian ini adalah skala fear of missing out dan skala kecanduan media sosial. Sampel dalam penelitian ini siswa SMP 17 Agustus 1945 Surabaya. Teknik pengambilan sampel pada penelitian ini menggunakan teknik quota sampling yang ditetapkan jumlahnya menggunakan bantuan tabel Krecjie sehingga diperoleh 162 subjek. Metode analisis data yang digunakan dalam penelitian ini menggunakan analisis korelasional Spearman Rho. Hasil penelitian ini menunjukkan yaitu bahwa hipotesis diterima dan terdapat hubungan positif antara fear of missing out dengan kecanduan media sosial.
Kata kunci: fear of missing out, kecanduan media sosial, remaja