bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contextualizing Parasympathetic Hyperactivity in Functionally Overreached Athletes With Perceptions of Training Tolerance
Authors: Bellenger Clint R. , Karavirta Laura , Thomson Rebecca L. , Robertson Eileen Y. , Davison Kade , Buckley Jonathan D. 
Published: December 2015

Purpose:Heart-rate variability (HRV) as a measure of autonomic function may increase in response to training interventions leading to increases or decreases in performance, making HRV interpretation difficult in isolation. This study aimed to contextualize changes in HRV with subjective measures of training tolerance.Methods:Supine and standing measures of vagally mediated HRV (root-mean-square difference of successive normal RR intervals [RMSSD]) and measures of training tolerance (Daily Analysis of Life Demands for Athletes questionnaire, perception of energy levels, fatigue, and muscle soreness) were recorded daily during 1 wk of light training (LT), 2 wk of heavy training (HT), and 10 d of tapering (T) in 15 male runners/triathletes. HRV and training tolerance were analyzed as rolling 7-d averages at LT, HT, and T. Performance was assessed after LT, HT, and T with a 5-km treadmill time trial (5TTT).Results:Time to complete the 5TTT likely increased after HT (effect size [ES] ± 90% confidence interval = 0.16 ± 0.06) and then almost certainly decreased after T (ES = −0.34 ± 0.08). Training tolerance worsened after HT (ES ≥ 1.30 ± 0.41) and improved after T (ES ≥ 1.27 ± 0.49). Standing RMSSD very likely increased after HT (ES = 0.62 ± 0.26) and likely remained higher than LT at the completion of T (ES = 0.38 ± 0.21). Changes in supine RMSSD were possible or likely trivial.Conclusion:Vagally mediated HRV during standing increased in response to functional overreaching (indicating potential parasympathetic hyperactivity) and also to improvements in performance. Thus, additional measures such as training tolerance are required to interpret changes in vagally mediated HRV.
Increase in Maximal Cycling Power With Acute Dietary Nitrate Supplementation
Authors: Rimer Ernest G. , Peterson Linda R. , Coggan Andrew R. , Martin James C. 
Published: December 2015

Muscle-shortening velocity and hence power have been shown to increase in the presence of nitric oxide (NO). NO availability increases after consuming nitrate (NO3-). Ingestion of NO3-rich beetroot juice (BRJ) has increased muscle power in untrained adults.Purpose:This study determined whether NO3- supplementation could acutely enhance maximal power in trained athletes.Methods:In this double-blind, crossover study, 13 trained athletes performed maximal inertial-load cycling trials (3–4 s) immediately before (PRE) and after (POST) consuming either NO3-rich (NO3) or NO3-depleted (PLA) BRJ to assess acute changes (ie, within the same day) in maximal power (PMAX) and optimal pedaling rate (RPMopt). Participants also performed maximal isokinetic cycling (30 s) to assess performance differences after supplementation.Results:2 x 2 repeated-measures ANOVA indicated a greater increase in PMAX from PRE to POST NO3 (PRE 1160 ± 301 W to POST 1229 ± 317 W) than with PLA (PRE 1191 ± 298 W to POST 1213 ± 300 W) (P = .009; ηp2 = 0.45). A paired t-test verified a greater relative change in PMAX after NO3 (6.0% ± 2.6%) than with PLA (2.0% ± 3.8%) (P = .014; d = 1.21). RPMopt remained unchanged from PRE (123 ± 14 rpm) to POST PLA (122 ± 14 rpm) but increased from PRE (120 ± 14 rpm) to POST NO3 (127 ± 13 rpm) (P = .043; ηp2 = 0.30). There was no relative change in RPMopt after PLA (–0.3% ± 4.1%), but there was an increase after NO3 (6.5% ± 11.4%) (P = .049; d = 0.79). No differences were observed between the 30-s isokinetic trials.Conclusions:Acute NO3- supplementation can enhance maximal muscle power in trained athletes. These findings may particularly benefit power-sport athletes who perform brief explosive actions.
Brief Morning Light Exposure, Visuomotor Performance, and Biochemistry in Sport Shooters
Authors: Leichtfried Veronika , Hanser Friedrich , Griesmacher Andrea , Canazei Markus , Schobersberger Wolfgang 
Published: December 2015

Context:Demands on concentrative and cognitive performance are high in sport shooting and vary in a circadian pattern, aroused by internal and external stimuli. The most prominent external stimulus is light. Bright light (BL) has been shown to have a certain impact on cognitive and physical performance.Purpose:To evaluate the impact of a single half hour of BL exposure in the morning hours on physical and cognitive performance in 15 sport shooters. In addition, courses of sulfateoxymelatonin (aMT6s), tryptophan (TRP), and kynurenine (KYN) were monitored.Methods:In a crossover design, 15 sport shooters were exposed to 30 min of BL and dim light (DL) in the early-morning hours. Shooting performance, balance, visuomotor performance, and courses of aMT6s, TRP, and KYN were evaluated.Results:Shooting performance was 365.4 (349.7–381.0) and 368.5 (353.9–383.1), identical in both light setups. Numbers of right reactions (sustained attention) and deviations from the horizontal plane (balance-related measure) were higher after BL. TRP concentrations decreased from 77.5 (73.5–81.4) to 66.9 (60.7–67.0) in the DL setup only.Conclusions:The 2 light conditions generated heterogeneous visuomotor and physiological effects in sport shooters. The authors therefore suggest that a single half hour of BL exposure is effective in improving cognitive aspects of performance, but not physical performance. Further research is needed to evaluate BL’s impact on biochemical parameters.
Nineteen Whole-Genome Assemblies of Yersinia pestis subsp.
microtus
, Including Representatives of Biovars caucasica, talassica, hissarica, altaica, xilingolensis, and ulegeica
Authors: Kislichkina Angelina A. , Bogun Aleksandr G. , Kadnikova Lidiya A. , Maiskaya Nadezhda V. , Platonov Mikhail E. , Anisimov Nikolai V. , Galkina Elena V. , Dentovskaya Svetlana V. , Anisimov Andrey P. 
Published: December 2015

ABSTRACT

The etiologic agent of plague,
Yersinia pestis
, includes two subspecies, of which
Y. pestis
subsp.
microtus
contains the strains that cause only occasional diseases in humans that are not accompanied by human-to-human transmission. Here, we report the draft genome sequences of 19
Y. pestis
strains (across 6 biovars of
Y. pestis
subsp.
microtus
).
Draft Genome Sequence of Agreia bicolorata Strain AC-1804, a Producer of Large Amounts of Carotenoid Pigments, Isolated from Narrow Reed Grass Infected by the Phytoparasitic Nematode
Authors: Vasilyev Ilya , Siniagina Maria , Malanin Sergey , Boulygina Eugenia , Grygoryeva Tatiana , Yarullina Dina , Ilinskaya Olga 
Published: December 2015

ABSTRACT

Here, we report the draft genome sequence of
Agreia bicolorata
strain AC-1804, isolated from narrow reed grass galls induced by a plant-parasitic nematode which is able to produce large amounts of carotenoid pigments. The draft genome sequence of 3,919,485 bp provides a resource for carotenoid pathway research.
Draft Genome Sequences of Three Mycobacterium chimaera Respiratory Isolates
Authors: Mac Aogáin Micheál , Roycroft Emma , Raftery Philomena , Mok Simone , Fitzgibbon Margaret , Rogers Thomas R. 
Published: December 2015

ABSTRACT

Mycobacterium chimaera
is an opportunistic human pathogen implicated in both pulmonary and cardiovascular infections. Here, we report the draft genome sequences of three strains isolated from human respiratory specimens.
Characterization and Sequencing of a Genotype VIId Newcastle Disease Virus Isolated from Laying Ducks in Jiangsu, China
Authors: Liu Mei , Shen Xinyue , Cheng Xu , Li Jianmei , Dai Yabin 
Published: December 2015

ABSTRACT
We report here the complete genome sequence and biological characterization of a virulent Newcastle disease virus (NDV) strain, NDV/duck/Jiangsu/JSD0812/2008, isolated from laying ducks in Jiangsu Province, China. The genome is 15,192 nucleotides in length and is classified in subgenotype VIId of genotype VII, class II.
The 474-Kilobase-Pair Complete Genome Sequence of CeV-01B, a Virus Infecting
Haptolina
(
Chrysochromulina
)
ericina
(Prymnesiophyceae)
Authors: Gallot-Lavallée Lucie , Pagarete António , Legendre Matthieu , Santini Sebastien , Sandaa Ruth-Anne , Himmelbauer Heinz , Ogata Hiroyuki , Bratbak Gunnar , Claverie Jean-Michel 
Published: December 2015

ABSTRACT

We report the complete genome sequence of CeV-01B, a large double-stranded DNA virus infecting the unicellular marine phytoplankton
Haptolina
(formerly
Chrysochromulina
)
ericina.
CeV-01B and its closest relative
Phaeocystis globosa
virus define an emerging subclade of the Megaviridae family with smaller genomes and particles than the originally described giant Mimiviridae infecting
Acanthamoeba
.
Complete Genome Sequence of Genotype Ib Newcastle Disease Virus Isolated from a Mallard (Anas platyrhynchos) in Russia
Authors: Yurchenko Kseniya S. , Sobolev Ivan A. , Glushchenko Alexandra V. , Shestopalov Alexander M. 
Published: December 2015

ABSTRACT
We report here the complete genome sequence of a Newcastle disease virus isolate, NDV/Yakutiya/mallard/852/2011, isolated from a mallard in Russia. On the basis of phylogenetic analysis, this strain was clustered into class II genotype Ib.
How Many Samples and How Many Culture Media To Diagnose a Prosthetic Joint Infection: a Clinical and Microbiological Prospective Multicenter Study
Authors: Bémer Pascale , Léger Julie , Tandé Didier , Plouzeau Chloé , Valentin Anne Sophie , Jolivet-Gougeon Anne , Lemarié Carole , Kempf Marie , Héry-Arnaud Geneviève , Bret Laurent , Juvin Marie Emmanuelle , Giraudeau Bruno , Corvec Stéphane , Burucoa Christophe ,   the Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Study Team
Published: December 2015

ABSTRACT
Although numerous perioperative samples and culture media are required to diagnose prosthetic joint infection (PJI), their exact number and types have not yet been definitely determined with a high level of proof. We conducted a prospective multicenter study to determine the minimal number of samples and culture media required for accurate diagnosis of PJI. Over a 2-year period, consecutive patients with clinical signs suggesting PJI were included, with five perioperative samples per patient. The bacteriological and PJI diagnosis criteria were assessed using a random selection of two, three, or four samples and compared with those obtained using the recommended five samples (references guidelines). The results obtained with two or three culture media were then compared with those obtained with five culture media for both criteria. The times-to-positivity of the different culture media were calculated. PJI was confirmed in 215/264 suspected cases, with a bacteriological criterion in 192 (89%). The PJI was monomicrobial (85%) or polymicrobial (15%). Percentages of agreement of 98.1% and 99.7%, respectively, for the bacteriological criterion and confirmed PJI diagnosis were obtained when four perioperative samples were considered. The highest percentages of agreement were obtained with the association of three culture media, a blood culture bottle, a chocolate agar plate, and Schaedler broth, incubated for 5, 7, and 14 days, respectively. This new procedure leads to significant cost saving. Our prospective multicenter study showed that four samples seeded on three culture media are sufficient for diagnosing PJI.
Depression and risk of hospitalisations and rehospitalisations for ambulatory care-sensitive conditions in Denmark: a population-based cohort study
Authors: Davydow Dimitry S , Fenger-Grøn Morten , Ribe Anette Riisgaard , Pedersen Henrik Søndergaard , Prior Anders , Vedsted Peter , Unützer Jürgen , Vestergaard Mogens 
Published: December 2015

ObjectiveHospitalisations for ambulatory care-sensitive conditions (ACSCs), a group of chronic and acute illnesses considered not to require inpatient treatment if timely and appropriate ambulatory care is received, and early rehospitalisations are common and costly. We sought to determine whether individuals with depression are at increased risk of hospitalisations for ACSCs, and rehospitalisation for the same or another ACSC, within 30 days.DesignNational, population-based cohort study.SettingDenmark.Participants5 049 353 individuals ≥18 years of age between 1 January 2005 and 31 December 2013.MeasurementsDepression was ascertained via psychiatrist diagnoses in the Danish Psychiatric Central Register or antidepressant prescription redemption from the Danish National Prescription Registry. Hospitalisations for ACSCs and rehospitalisations within 30 days were identified using the Danish National Patient Register.ResultsOverall, individuals with depression were 2.35 times more likely to be hospitalised for an ACSC (95% CI 2.32 to 2.37) versus those without depression after adjusting for age, sex and calendar period, and 1.45 times more likely after adjusting for socioeconomic factors, comorbidities and primary care utilisation (95% CI 1.43 to 1.46). After adjusting for ACSC-predisposing comorbidity, depression was associated with significantly greater risk of hospitalisations for all chronic (eg, angina, diabetes complications, congestive heart failure exacerbation) and acute ACSCs (eg, pneumonia) compared to those without depression. Compared to those without depression, persons with depression were 1.21 times more likely to be rehospitalised within 30 days for the same ACSC (95% CI 1.18 to 1.24) and 1.19 times more likely to be rehospitalised within 30 days for a different ACSC (95% CI 1.15 to 1.23).ConclusionsIndividuals with depression are at increased risk of hospitalisations for ACSCs, and once discharged are at elevated risk of rehospitalisations within 30 days for ACSCs.
Software Engineering Project Courses with Industrial Clients
Authors: Bruegge Bernd , Krusche Stephan , Alperowitz Lukas 
Published: December 2015

There is an acknowledged need for teaching realistic software development in project courses. The design space for such courses is wide, ranging from single-semester to two-semester courses, from single-client to multicustomer courses, from local to globally distributed courses, and from toy projects to projects with real clients. The challenge for a nontrivial project course is how to make the project complex enough to enrich students’ software engineering experience, yet realistic enough to have a teaching environment that does not unduly burden students or the instructor. We describe a methodology for project courses that is realizable for instructors, improves students’ skills, and leads to viable results for industry partners.
In particular, recent advances in release management and collaboration workflows reduce the effort of students and instructors during delivery and increase the quality of the deliverables. To enable release and feedback management, we introduce Rugby, an agile process model based on Scrum that allows reacting to changing requirements. To improve early communication, we use Tornado, a scenario-based design approach that emphasizes the use of informal models for the interaction between clients and students. The combination of Rugby and Tornado allows students to deal with changing requirements, produce multiple releases, and obtain client feedback through the duration of the course.
We describe our experience with more than 300 students working on 40 projects with external clients over a 4-year period. In the latest instance of our course, the students have produced more than 7000 builds with 600 releases for eleven clients. In an evaluation of the courses, we found that the introduction of Rugby and Tornado significantly increased students’ technical skills, especially with respect to software engineering, usability engineering, and configuration management, as well as their nontechnical skills such as communication with the client, teamwork, presentation, and demo management. Finally, we discuss how other instructors can adapt the course concept.
Bereavement Is Associated with an Increased Risk of HPV Infection and Cervical Cancer: An Epidemiological Study in Sweden
Authors: Lu Donghao , Sundström Karin , Sparén Pär , Fall Katja , Sjölander Arvid , Dillner Joakim , Helm Nathalie Ylitalo , Adami Hans-Olov , Valdimarsdóttir Unnur , Fang Fang 
Published: December 2015

Abstract
Grief over the loss of a family member may cause physical and mental illness, but an association between bereavement and cancer risk has not been established. Based on the Swedish National Cervical Screening Register (1969–2011) including 14,011,269 smears from 2,466,107 women, we conducted two nested case–control studies to examine the associations of bereavement (i.e., loss of a family member due to death) with abnormal cytology (390,310 first abnormal and 1,951,319 normal smears) and in situ/invasive cervical cancer (75,128 case and 375,640 control women), both individually matched on year of birth and screening adherence. Among 1,696 of the control women, we further investigated bereavement in association with human papillomavirus (HPV) infection, both HPV16 and other HPV types. Bereavement was consistently associated with a 4% to 9% increased risk for first abnormal cytology, in situ and invasive cervical cancer (all P &lt; 0.02). The associations became stronger when multiple losses, loss of child, sibling or spouse, and loss due to unnatural cause were analyzed separately (P for trend or difference &lt; 0.0001), and for women with high screening adherence (P for difference &lt; 0.05). Among 1,696 women who had not developed cervical cancer, we further investigated the link between bereavement and HPV infection. Bereavement was associated with a 62% increased risk of HPV16 infection, high viral load, and recurrent infection, and was also more strongly associated with HPV infections designated as high-risk compared with low-risk determinants of cervical carcinogenesis. Collectively, our findings demonstrate that bereavement is associated with an increased risk of developing cervical cancer. Further, they suggest that this association may be attributed to stress-induced oncogenic HPV infections. Cancer Res; 76(3); 643–51. ©2015 AACR.
Selective Toxicity of Investigational Ixazomib for Human Leukemia Cells Expressing Mutant Cytoplasmic NPM1: Role of Reactive Oxygen Species
Authors: Garcia Jacqueline S. , Huang Min , Medeiros Bruno C. , Mitchell Beverly S. 
Published: December 2015

Abstract
Purpose: This study was performed to determine whether the investigational proteasome inhibitor ixazomib demonstrated selective antineoplastic activity against acute myelogenous leukemia cells expressing a mutated nucleophosmin-1 gene and to gain a better understanding of its mechanisms of action.
Experimental Design: The cytotoxic effects of ixazomib treatment were analyzed in human acute myelogenous leukemia (AML) cell lines and primary AML samples expressing wild-type or mutated NPM1 (NPMc+). The potential roles of oxidative stress in mediating cytotoxic activity were determined using flow cytometry, enzyme-based assays, and Western blots.
Results: Apoptosis induced by ixazomib was abrogated by knockdown of NPM1/NPMc+ expression using an inducible shRNA construct and enhanced by NPMc+ overexpression. Cytotoxicity was associated with superoxide generation and was reduced by the addition of the antioxidant N-acetylcysteine. AML cells expressing NPMc+ had significantly reduced levels of intracellular glutathione and NADPH associated with reduced antioxidant responses to drug treatment. Treatment of 3 patients with relapsed NPMc+ AML resulted in an antileukemic effect in 1 patient as demonstrated by a marked reduction of leukemic blasts in the peripheral blood. Efficacy was associated with superoxide generation, reduced glutathione levels, and reduced mRNA and protein expression of antioxidant effectors in responding cells.
Conclusions: In this study, a direct association was observed between NPMc+ expression in AML, reduced antioxidant responses, and enhanced sensitivity to an oral proteasome inhibitor that induces oxidative stress. These data suggest that intracellular determinants of antioxidant responses may be good predictors of therapeutic response to ixazomib. Clin Cancer Res; 22(8); 1978–88. ©2015 AACR.
Overcoming Antigen Escape with CAR T-cell Therapy
Authors: Jackson Hollie J. , Brentjens Renier J. 
Published: December 2015

Abstract
Summary: Sotillo and colleagues describe the molecular events associated with apparent loss of target antigen expression following CAR T-cell therapy. We propose that broader immune activation is required to prevent outgrowth of tumor antigen escape variants following targeted therapies. Cancer Discov; 5(12); 1238–40. ©2015 AACR.
See related article by Sotillo et al., p. 1282.
LRP1 Regulates Retinal Angiogenesis by Inhibiting PARP-1 Activity and Endothelial Cell Proliferation
Authors: Mao Hua , Lockyer Pamela , Townley-Tilson W.H. Davin , Xie Liang , Pi Xinchun 
Published: December 2015

Objective—
We recently demonstrated that low-density lipoprotein receptor–related protein 1 (LRP1) is required for cardiovascular development in zebrafish. However, what role LRP1 plays in angiogenesis remains to be determined. To better understand the role of LRP1 in endothelial cell function, we investigated how LRP1 regulates mouse retinal angiogenesis.

Approach and Results—
Depletion of LRP1 in endothelial cells results in increased retinal neovascularization in a mouse model of oxygen-induced retinopathy. Specifically, retinas in mice lacking endothelial LRP1 have more branching points and angiogenic sprouts at the leading edge of the newly formed vasculature. Increased endothelial proliferation as detected by Ki67 staining was observed in LRP1-deleted retinal endothelium in response to hypoxia. Using an array of biochemical and cell biology approaches, we demonstrate that poly(ADP-ribose) polymerase-1 (PARP-1) directly interacts with LRP1 in human retinal microvascular endothelial cells. This interaction between LRP1 and PARP-1 decreases under hypoxic condition. Moreover, LRP1 knockdown results in increased PARP-1 activity and subsequent phosphorylation of both retinoblastoma protein and cyclin-dependent kinase 2, which function to promote cell cycle progression and angiogenesis.

Conclusions—
Together, these data reveal a pivotal role for LRP1 in endothelial cell proliferation and retinal neovascularization induced by hypoxia. In addition, we demonstrate for the first time the interaction between LRP1 and PARP-1 and the LRP1-dependent regulation of PARP-1–signaling pathways. These data bring forth the possibility of novel therapeutic approaches for pathological angiogenesis.