bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Dosis Pupuk Urea Dan Konsentrasi Pupuk Organik Cair Nasa Terhadap Pertumbuhan Dan Hasil Tanaman Selada (Lactuca sativa L.)
Authors: Husna Rika , Yasir Afif Muhammad , Rahmawati Marai 
Published: September 2024

Penelitian ini bertujuan untuk mengetahui pengaruh dosis Urea dan konsentrasi Pupuk Organik Cair NASA terhadap pertumbuhan dan hasil tanaman selada (Lactuca sativa L.) di dataran tinggi Gayo. Penelitian ini dilaksanakan dari bulan Januari sampai dengan bulan Maret 2021 berlokasi di lahan milik warga yang berlokasi Tetunyung Kampung Takengon Timur, Kecamatan Lut Tawar, Kabupaten Aceh Tengah. Penelitian ini menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor, yang pertama adalah dosis pupuk Urea yaitu tanpa pupuk Urea Kontrol (U0), 100 kg/ha (U1), 150 kg/ha (U2), and 200 kg/ha (U3) sedangkan faktor kedua adalah konsentrasi POC NASA yaitu tanpa POC NASA 0 ml/l (N0), 3 ml/l (N1), 6ml/l (N2) dan 9 ml/l (N3). Hasil penelitian didapatkan bahwa perlakuan dosis Urea memberi pengaruh sangat nyata pada tinggi tanaman 14HST, 21HST dan 28HST , jumlah daun 14HST, 21HST dan 28HST, panjang daun, lebar daun, berat berangkasan basah dan hasil per hektar Dosis pupuk Urea terbaik adalah Urea 100 kg/ha menunjukkan rata-rata hasil per hektar tertinggi yaitu 23,50 ton/ha. Perlakuan konsentrasi POC NASA memberikan pengaruh sangat nyata pada parameter tinggi tanaman, jumlah daun, panjang daun, lebar daun, berat berangkasan basah, dan potensi hasil per hektar. Konsentrasi POC NASA terbaik yaitu 3 ml/L dengan hasil per hektar tertinggi yaitu 15,67 ton/ha. Terdapat interaksi yang nyata antara dosis Pupuk Urea dan konsentrasi POC NASA parameter tinggi tanaman umur 14HST, 21HST dan 28 HST, jumlah daun 14HST, 21HST dan 28HST , panjang daun, lebar daun, berat berangkasan basah, dan hasil per hektar. Kombinasi dosis pupuk Urea dan konsentrasi POC NASA yang terbaik adalah pupuk Urea 100 kg/ha dan konsentrasi POC NASA 3 ml/L memberikan hasil per hektar terbaik yaitu 30,95 ton/ha.
Educación artística STEAM en la formación universitaria. Robótica creativa para la alfabetización visual y digital
Authors: Soto Solier Pilar Manuela , Villena Soto Verónica 
Published: September 2024

Introducción: Ante una sociedad digitalizada que demanda propuestas educativas que incorporen la alfabetización visual y las TIC en las aulas nace esta investigación con el objetivo de evaluar la eficacia de un programa educativo audiovisual STEAM dirigido a la mejora de las competencias audiovisuales y digitales del alumnado. Metodología: En este estudio el alumnado del Grado en Educación Primaria (N=67) elabora storytelling utilizando ilustración digital y robótica creativa (Scratch) para reflexionar sobre los retos de la sociedad actual. Se aplica una metodología basada en las Artes Visuales y el Aprendizaje Basado en Proyectos STEAM. Los datos son recogidos mediante un cuestionario estandarizado pre- y post- formación y una rúbrica para la evaluación de las videoanimaciones interactivas creadas por el alumnado. Resultados: Los resultados revelan diferencias entre los cuestionarios pre- y post- formación, mejoras en actitud y conocimientos tras la formación. La evaluación de las videoanimaciones interactivas muestra una mejora en las competencias audiovisuales y digitales y en las habilidades críticas del alumnado. Conclusiones: La investigación muestra la eficacia del aprendizaje interdisciplinar basado en las Artes Visuales y la pedagogía STEAM, pero también la necesidad de continuar indagando en nuevas metodologías para afrontar los retos de la sociedad.
The Effect of FTY720 on Sphingolipid Imbalance and Cognitive Decline in Aged EFAD Mice
Authors: Luo Qian , Crivelli Simone M. , Zong Shenghua , Giovagnoni Caterina , van Kruining Daan , Mané-Damas Marina , den Hoedt Sandra , Berkes Dusan , De Vries Helga E. , Mulder Monique T. , Walter Jochen , Waelkens Etienne , Derua Rita , Swinnen Johannes V. , Dehairs Jonas , Losen Mario , Martinez-Martinez Pilar 
Published: September 2024

Background: During Alzheimer’s disease (AD) progression, there is a decline in the bioactive sphingolipid sphingosine-1-phosphate (S1P). Previous research showed that FTY720, an S1P mimetic, prevented cognitive decline and reduced ceramide levels in transgenic mice with familial AD carrying the human APOE4 gene (E4FAD) at 6–7 months of age. Objective: The objective of this study is to explore the protective effects of FTY720 at late-stage AD. Methods: Male mice aged 9.5 to 10.5 months were orally administered FTY720 (0.1 mg/kg) via oral gavage for 6 weeks. A pre-test of water maze was used for evaluating the pathological status. After 4 weeks of administration, memory, locomotion, and anxiety were assessed. Cortex samples were analyzed for amyloid-β (Aβ) and sphingolipid levels. Results: Compared with APOE3 mice, APOE4, E3FAD and E4FAD mice exhibited significant memory deficits. After 6 weeks administration, FTY720 did not alleviate memory deficits in EFAD mice. Lipid analysis revealed that S1P was significantly reduced in EFAD mice (E3FAD or E4FAD) compared to controls (APOE3 and APOE4). Ceramide level alterations were predominantly dependent on APOE isoforms rather than AD transgenes. Interestingly, Cer (d18 : 1/22 : 1) was elevated in APOE4 mice compared to APOE3, and FTY720 reduced it. Conclusions: E4FAD and APOE4 mice exhibited significant spatial memory deficits and higher ceramide concentrations compared to APOE3 mice. FTY720 did not reverse memory deficits in E4FAD and APOE4 mice but reduced specific ceramide species. This study provides insights into the association between sphingolipids and APOE4 in advanced AD stages, exploring potential therapeutic targeting of sphingolipid metabolism.
Individualized and Biomarker-Based Prognosis of Longitudinal Cognitive Decline in Early Symptomatic Alzheimer’s Disease
Authors: Wang Xiwu , Ye Teng , Huang Ziye , Zhou Wenjun , Zhang Jie ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: September 2024

Background: Although individualized models using demographic, MRI, and biological markers have recently been applied in mild cognitive impairment (MCI), a similar study is lacking for patients with early Alzheimer’s disease (AD) with biomarker evidence of abnormal amyloid in the brain. Objective: We aimed to develop prognostic models for individualized prediction of cognitive change in early AD. Methods: A total of 421 individuals with early AD (MCI or mild dementia due to AD) having biomarker evidence of abnormal amyloid in the brain were included in the current study. The primary cognitive outcome was the slope of change in Alzheimer’s Disease Assessment Scale-cognitive subscale-13 (ADAS-Cog-13) over a period of up to 5 years. Results: A model combining demographics, baseline cognition, neurodegenerative markers, and CSF AD biomarkers provided the best predictive performance, achieving an overfitting-corrected R2 of 0.59 (bootstrapping validation). A nomogram was created to enable clinicians or trialists to easily and visually estimate the individualized magnitude of cognitive change in the context of patient characteristics. Simulated clinical trials suggested that the inclusion of our nomogram into the enrichment strategy would lead to a substantial reduction of sample size in a trial of early AD. Conclusions: Our findings may be of great clinical relevance to identify individuals with early AD who are likely to experience fast cognitive deterioration in clinical practice and in clinical trials.
Assessment of decellularization strategy and biocompatibility testing of full-thickness abdominal wall to produce a tissue-engineered graft
Authors: Skepastianos George , Mallis Panagiotis , Kostopoulos Epameinondas , Michalopoulos Efstathios , Skepastianos Vasileios , Doudakmanis Christos , Palazi Chrysoula , Tsourouflis Gerasimos 
Published: September 2024

Background Restoration of the abdominal wall defects due to herniation or other complications represents a challenging task of the reconstructive surgery. Synthetic grafts or crosslinked animal-derived grafts, are utilized, followed by significant adverse reactions. Objective This study aimed to evaluate primarily the production of a decellularized abdominal wall scaffold and secondly its biocompatibility upon transplantation in an animal model. Methods Full-thickness abdominal wall samples were harvested from Wistar Rats and then decellularized utilizing a three-cycle process. To evaluate the decellularization efficacy, histological, biochemical and biomechanical analyses were performed. The biocompatibility assessment involved the implantation of the produced scaffolds to Sprague Dawley rats. The grafts remained for a total period of 4 weeks, followed by immunohistochemistry for the detection of CD11b+, CD4+ and CD8+ cells. Results Histological, biochemical and biomechanical results, indicated the production of compatible acellular full-thickness abdominal wall samples. After 4 weeks of implantation, a minor presence of immunity cells was observed. Conclusion The data of this study indicated the successful production of a full-thickness abdominal wall scaffold. Biologically derived full-thickness abdominal wall scaffolds may have greater potential in restoration of the abdominal wall defects, bringing them one step closer to their clinical utility.
Regulation of cagA-Helicobacter on gastric PIM2 expression in gastric cancer
Authors: Liang Wenjuan , Wang Haiyan , Hong Lijuan , Cao Wenting , Chen Shuaiyin , Zhang Rongguang 
Published: September 2024

BACKGROUND: The association between infection with cagA-positive H. pylori and an elevated susceptibility to gastric cancer has been firmly established. PIM2 is known to be overexpressed in various types of cancers; however, the specific mechanism by which cagA influences the regulation of PIM2 expression in gastric cancer remains unidentified at present. MATERIALS AND METHODS: A mutant NCTC11637[Formula: see text]cagA strain of H. pylori and the eukaryotic expression vector pcDNA- cagA were constructed for evaluating PIM2 expression levels in gastric cancer cells (HGC27, SGC7901, and AG) co-cultured with the NCTC11637 and NCTC11637[Formula: see text]cagA strain, as well as pcDNA- cagA and the empty vector pcDNA3.1 ([Formula: see text]). RESULTS: Co-culturing gastric cancer cells with NCTC11637 significantly increased PIM2 expression levels ([Formula: see text] 0.001) compared to the negative control group. Additionally, the expression of PIM2 in cells co-cultured with NCTC11637 was higher than that co-cultured with NCTC11637[Formula: see text]cagA ([Formula: see text] 0.001). Furthermore, successful construction of the eukaryotic expression vector pcDNA- cagA resulted in a significant increase in PIM2 mRNA expression levels after its transfection into gastric cancer cells compared to the control group after 48 hours. CONCLUSIONS: The findings indicate that H. pylori/ cagA A could be one of the key factors in regulating PIM2 expression levels, potentially influencing the progression of H. pylori-related Gastric Cancer.
Validation Study of the Parkinson’s Disease Stigma Questionnaire (PDStigmaQuest)
Authors: Stopic Vasilija , Jost Stefanie T. , Haupt Julius , Brandt Gregor A. , van der Linden Christina , Petry-Schmelzer Jan Niklas , Dembek Till A. , Fink Gereon R. , Batzu Lucia , Rizos Alexandra , Chaudhuri K. Ray , Dafsari Haidar S. , Gruber Doreen , Ebersbach Georg , Kessler Josef , Barbe Michael T. , Sauerbier Anna 
Published: September 2024

Background: Stigma is a relevant aspect of Parkinson’s disease (PD). Specific stigma tools are needed to address the complex construct of stigma in PD comprehensively. Objective: To test the dimensionality and psychometric properties of the newly developed Parkinson’s Disease Stigma Questionnaire (PDStigmaQuest). Methods: In this multi-center, cross-sectional study including PD patients and healthy controls, the dimensionality of the PDStigmaQuest was examined through exploratory factor analysis. Acceptability and psychometric properties were investigated. PDStigmaQuest scores of patients and healthy controls were compared. Results: In total, 201 PD patients and 101 healthy controls were included in the final analysis. Results suggested high data quality of the PDStigmaQuest (0.0001% missing data for patients). The exploratory factor analysis produced four factors: felt stigma, hiding, enacted stigma: rejection, and enacted stigma: patronization, explaining 47.9% of variance. An optional work domain for employed patients was included. Moderate floor effects and skewness, but no ceiling effects were found. Cronbach’s alpha of 0.85 indicated high internal consistency. Calculated item-total correlations met standard criteria. Test-retest reliability was high ( rs = 0.83). PDStigmaQuest scores correlated significantly with other stigma measures ( rs = 0.56–0.69) and were significantly higher in patients than in healthy controls and higher in patients with depressive symptoms than in those without. Conclusions: The patient-reported 18-item PDStigmaQuest showed strong psychometric properties of validity and reliability. Our results suggest that the PDStigmaQuest can be used to assess and evaluate stigma comprehensively in PD, which will improve our understanding of the construct of PD stigma.
PENGGUNAAN KAWASAN HUTAN UNTUK KEPENTINGAN PENGUSAHAAN PANAS BUMI TIDAK LANGSUNG: TELAAH ALIRAN HUKUM ALAM
Authors: Suryono Ahmad , Rato Dominikus , Dwi Anggono Bayu 
Published: September 2024

Sebaran potensi panas bumi untuk pengusahaan tidak langsung yang meliputi tujuan pembangkit listrik tersebar di wilayah dan kawasan yang selektif dan terlarang untuk digunakan, seperti hutan lindung, hutan koservasi, dan zona inti pada Taman Nasional. Penggunaan kawasan hutan untuk kepentingan pembangunan di luar kegiatan kehutanan secara limitatif diatur di UU Kehutanan yang kemudian dirubah dengan UU Cipta Kerja. Â Jenis penelitian adalah penelitian normatif, dengan pendekatan conseptual approach dan statute approach. Teori Hukum Alam yang diusung oleh Aquinas dan Hart memotret fenomena pergeseran penggunaan kawasan hutan untuk pengusahaan panas bumi tidak langsung sebagai suatu hubungan yang komprehensif dan menjiwai hukum positif pengaturan penggunaan kawasan hutan untuk pengusahaan panas bumi tidak langsung
EDITORIAL SCOPE: BEYOND RESILIENCE – INDEX FOR THE NEXT-GENERATION OF SEISMIC VULNERABILITY FOR RC STRUCTURE
Authors: Kassem Moustafa Moufid , Mohamed Nazri Fadzli 
Published: September 2024

Seismic vulnerability assessment has become an indispensable tool in earthquake engineering, especially for reinforced concrete (RC) structures, which are prevalent in urbanized regions worldwide. In this editorial note, we expand on key contributions from recent papers published in the Journal of Building Engineering and Journal of Earthquake Engineering, merging these insights with findings from the book "Seismic Vulnerability Index Assessment Framework of RC Structures." Together, these works form a cohesive narrative around the development of a more refined and globally applicable Seismic Vulnerability Index (SVI) framework for RC structures. Moreover, this editorial note discusses the advancements in the seismic vulnerability assessment of RC buildings through the development of an SVI methodology. This methodology uses advanced nonlinear parametric analyses to quantify the seismic vulnerability of RC buildings, contributing significantly to disaster risk reduction efforts. Thus, the contribution lies in developing improved methodologies for assessing seismic vulnerability and quantifying seismic risk, ultimately aiding in enhancing earthquake resilience. This comprehensive framework is globally applicable, adaptable to any geographical region, and suitable for a wide range of structural types and systems.
Projected changes to Arctic shipping routes after stratospheric aerosol deployment in the ARISE-SAI scenarios
Authors: Morrison Ariel L. , Pathak Debanjali , Barnes Elizabeth A. , Hurrell James W. 
Published: September 2024

IntroductionRapid reductions in Arctic sea ice in response to warming have led to increased interest in using the Arctic Ocean for commercial shipping. As the world warms, however, different strategies are being considered to stabilize or reduce surface temperatures in order to prevent critical climate change impacts. One such strategy is stratospheric aerosol injection (SAI), a form of solar climate intervention. Projected changes to Arctic sea ice under SAI with specific regards to shipping have not yet been assessed.MethodsWe compare output from two SAI simulations that have different global mean temperature targets with a non-SAI control simulation to provide the first assessment of Arctic Ocean navigability under potential SAI scenarios.ResultsWe find that sea ice concentration and thickness quickly stabilize or increase after SAI deployment. When sea ice thickness stabilizes in response to SAI, the number of days when the Arctic Ocean is navigable remains fairly constant, but increasing sea ice thickness leads to reduced navigability compared to the non-SAI simulation. From 2035-2069, both the Northwest Passage and Northern Sea Route are accessible from July-November in all three simulations, but there are no navigable routes under either SAI scenario from April-June. When the Arctic is navigable, it can take 2-12 days longer to cross the Arctic Ocean in the SAI simulations than in the non-SAI control simulation, and there are large year-to-year variations in travel time.DiscussionOverall, Arctic shipping may take longer and be more difficult in an SAI vs a non-SAI world because of relatively thicker sea ice, but the degree to which Arctic shipping may change in response to SAI is dependent on the particular climate intervention strategy.
Citizen science in elementary classrooms: a tale of two teachers
Authors: Carrier Sarah J. , Scharen Danielle R. , Hayes Meredith , Smith P. Sean , Bruce Anna , Craven Laura 
Published: September 2024

IntroductionElementary teachers face many challenges when including reform-based science instruction in their classrooms, and some teachers have chosen to enhance their science instruction by introducing students to citizen science (CS) projects. When CS projects are incorporated in formal school settings, students have an opportunity to engage in real-world projects as they collect and make sense of data, yet relatively few CS projects offer substantial guidance for teachers seeking to implement the projects, placing a heavy burden on teacher learning.MethodsFramed in theory on teacher relationships with curricula, we prepared science standards-aligned educative support materials for two CS projects. We present convergent mixed methods research that examines two teachers’ contrasting approaches to including school-based citizen science (SBCS) in their fifth-grade classrooms, each using support materials for one of the two CS projects. Both are veteran teachers at under-resourced Title 1 (an indicator of the high percentage of the students identified as economically disadvantaged) rural schools in the southeastern United States. We document the teachers’ interpretations and use of SBCS materials for the CS projects with data from classroom observations, instructional logs, teacher interviews, and student focus groups.ResultsOne teacher adapted the materials to include scaffolding to position students for success in data collection and analysis. In contrast, the second teacher adapted the SBCS support materials to maintain a teacher-centered approach to instruction, identifying perceptions of students’ limited abilities and limited instructional time as constraining factors.DiscussionWe discuss the intersection of CS projects in formal education and opportunities for engaging students in authentic science data collection, analysis, and sense-making. The two teachers’ stories identify the influences of school context and the need for teacher support to encourage elementary teachers’ use of SBCS instruction to supplement their science instruction.
Gut microbiota and interstitial cystitis: exploring the gut-bladder axis through mendelian randomization, biological annotation and bulk RNA sequencing
Authors: Fu Chaowei , Zhao Yu , Zhou Xiang , Lv Jing , Jin Shengkai , Zhou Yuhua , Liu Fengping , Feng Ninghan 
Published: September 2024

BackgroundSeveral observational studies have indicated an association between interstitial cystitis and the composition of the gut microbiota; however, the causality and underlying mechanisms remain unclear. Understanding the link between gut microbiota and interstitial cystitis could inform strategies for prevention and treatment.MethodsA two-sample Mendelian randomization analysis was conducted using published genome-wide association study summary statistics. We employed inverse variance weighted, weighted mode, MR-Egger, weighted median, simple mode, and cML-MA methods to investigate the causal relationship between gut microbiota and interstitial cystitis. Sensitivity analysis was performed to validate the results. Relevant gut microbiota was examined through reverse MR. Single nucleotide polymorphisms were annotated using FUMA to identify genes associated with these genetic variants, thereby revealing potential host gene-microbiota associations in interstitial cystitis patients.ResultsEight bacterial taxa were identified in our analysis as associated with interstitial cystitis. Among these, Butyricimonas, Coprococcus, Lactobacillales, Lentisphaerae, and Bilophila wadsworthia were positively correlated with interstitial cystitis risk, while taxa such as Desulfovibrio piger, Oscillibacter unclassified and Ruminococcus lactaris exhibited protective effects against interstitial cystitis. The robustness of these associations was confirmed through sensitivity analyses. Reverse MR analysis did not reveal evidence of reverse causality. Single nucleotide polymorphisms were annotated using FUMA and subjected to biological analysis. Seven hub genes (SPTBN1, PSME4, CHAC2, ERLEC1, ASB3, STAT5A, and STAT3) were identified as differentially expressed between interstitial cystitis patients and healthy individuals, representing potential therapeutic targets.ConclusionOur two-sample Mendelian randomization study established a causal relationship between gut microbiota and interstitial cystitis. Furthermore, our identification of a host gene-microbiota association offers a new avenue for investigating the potential pathogenesis of interstitial cystitis and suggests avenues for the development of personalized treatment strategies.