bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteinase‐Activated Receptor‐2 Modulates Ve‐Cadherin Expression to Affect Human Vascular Endothelial Barrier Function
Authors: Zhang Rui , Ge Jianjun 
Published: May 2017

ABSTRACTPublished data indicate that the protease‐activated receptor (PAR) 2 is involved in the pathogenesis of some cardiovascular diseases; the underlying mechanism is to be further investigated. Ve‐cadherin is a critical molecule in maintaining the endothelial barrier integrity. This study aims to investigate the role of PAR2 activation in compromising the cardiac endothelial barrier function. In this study, human umbilical vein endothelial cells (Huvec cells) were cultured into monolayers using as an in vitro model of barrier function. The transepithelial electric resistance (TER) and permeability to dextran were assessed as indicators of barrier function. The expression of Ve‐cadherin in Huvec cells was assessed by real‐time RT‐PCR, Western blotting, and chromatin immunoprecipitation. The results showed that exposure to tryptase in the culture, the barrier function of the Huvec monolayers, was markedly compromised; the levels of Ve‐cadherin, one of the tight junction proteins, were suppressed as well. This was mimicked by exposing Huvec monolayers to the active PAR2 peptides (PAR2AP). After exposing to PAR2AP, the levels of histone deacetylase (HDAC)11 were increased in the Huvec cells. HDAC11 formed a complex with the transcription factor of Ve‐cadherin to attenuate the Erg gene transcription activities and suppressed the expression of Ve‐cadherin. In conclusion, activation of PAR2 compromises the vascular endothelial barrier function by suppressing the expression of Ve‐cadherin. J. Cell. Biochem. 118: 4587–4593, 2017. © 2017 Wiley Periodicals, Inc.
Mechanistic Principles Behind Molecular Mechanism of Rifampicin Resistance in Mutant RNA Polymerase Beta Subunit of Mycobacterium tuberculosis
Authors: Singh Aditi , Grover Sonam , Sinha Siddharth , Das Mriganko , Somvanshi Pallavi , Grover Abhinav 
Published: May 2017

ABSTRACTEvolution of drug‐resistant Mycobacterium strains threatens the TB treatment and control programs globally. Rifampicin (RIF) is an important first line antitubercular drug. Resistance to Rifampicin is caused mainly by mutations in its target RNA polymerase beta subunit protein (RpoB). RpoB contains a Rifampicin resistance determining region (RRDR) and has several potent sites for mutations. In this study, we have investigated mutations of a single site (H451) to eight different amino acids, involved in RIF resistance. Long‐term molecular dynamics simulations were performed on wild type (WT) and mutant protein structures and various structural analysis were carried out to elucidate the dynamic behavior of WT and mutant forms. Essential dynamics uncovered the difference in conformational flexibility and collective modes of motions between WT and mutants. MMPBSA calculations and interaction pattern analysis revealed the binding site relocation in some mutants. This study presents an exhaustive analysis of RIF binding to the WT and mutant RpoB and clearly highlights structural mechanism for differences in stable binding of Rifampicin with WT than the mutant targets. J. Cell. Biochem. 118: 4594–4606, 2017. © 2017 Wiley Periodicals, Inc.
miR‐34a Targets HDAC1‐Regulated H3K9 Acetylation on Lipid Accumulation Induced by Homocysteine in Foam Cells
Authors: Zhao Qingguo , Li Shuqiang , Li Nan , Yang Xiaoling , Ma Shengchao , Yang Anning , Zhang Hui , Yang Songhao , Mao Caiyan , Xu Lingbo , Gao Tingting , Yang Xiaoming , Zhang Huiping , Jiang Yideng 
Published: May 2017

ABSTRACTHyperhomocysteinemia (HHcy) promotes atherogenesis by modification of histone acetylation patterns and regulation of miRNA expression while the underlying molecular mechanisms are not well known. In this study, we investigated the effects of homocysteine (Hcy) on the expression of histone deacetylase 1 (HDAC1) and tested our hypothesis that Hcy‐induced atherosclerosis is mediated by increased HDAC1 expression, which is regulated by miR‐34a. The expression of HDAC1 increased and acetylation of histone H3 at lysine 9 (H3K9ac) decreased in the aorta of ApoE−/− mice fed with high methionine diet, whereas miR‐34a expression was inhibited. Over‐expression of HDAC1 inhibited H3K9ac level and promoted the accumulation of total cholesterol, free cholesterol, and triglycerides in the foam cells. Furthermore, up‐regulation of miR‐34a reduced HDAC1 expression and inhibited the accumulation of total cholesterol (TC), free cholesterol (FC), and triglycerides (TG) in the foam cells. These data suggest that HDAC1‐related H3K9ac plays a key role in Hcy‐mediated lipid metabolism disorders, and that miR‐34a may be a novel therapeutic target in Hcy‐related atherosclerosis. J. Cell. Biochem. 118: 4617–4627, 2017. © 2017 Wiley Periodicals, Inc.
Necroptosis Resumes Apoptosis in Hippocampus but Not in Frontal Cortex
Authors: Nikseresht Sara , Khodagholi Fariba , Dargahi Leila , Ahmadiani Abolhassan 
Published: May 2017

ABSTRACTCell death subsequent to or concurrent with neuroinflammation results in some damages like neuron loss and spatial memory impairment. In this study, we demonstrated the temporal pattern of neuroinflammation, necroptotic, and apoptotic cell deaths in hippocampus and frontal cortex following intracerebroventricular administration of lipopolysaccharide (LPS). We evaluated receptor interacting protein kinase 1 (RIP1), RIP3, and two related metabolic enzymes including glutamate‐ammonia ligase (GLUL) and glutamate dehydrogenase (GLUD) as necroptosis factors. Apoptosis pathway, antioxidant status and inflammatory cytokines were also assessed. Based on the probable role of these brain regions in working memory performance, spontaneous alternation was evaluated through the Y‐maze apparatus. RIP1, RIP3, and then GLUL and GLUD, as well as apoptosis markers, inflammatory regulators, and antioxidant defense demonstrated different time‐dependent patterns in hippocampus and frontal cortex. Interestingly, in hippocampus but not in frontal cortex, necroptosis resumed apoptosis. Our results in behavioral section revealed that neuroinflammation along with apoptosis and necroptosis pathways could lead to reversible short‐term memory impairment after LPS injection. In conclusion, it can be suggested that there is a region‐specific response of cell deaths regulators activation in hippocampus and frontal cortex. In addition, elucidating the time profile of events in response to neuroinflammation would be of great help in mechanistic studies and understanding of pathways interaction. J. Cell. Biochem. 118: 4628–4638, 2017. © 2017 Wiley Periodicals, Inc.
Shikonin Inhibited Migration and Invasion of Human Lung Cancer Cells via Suppression of c‐Met‐Mediated Epithelial‐to‐Mesenchymal Transition
Authors: Hsieh Yei‐San , Liao Chiung‐Ho , Chen Wan‐Shen , Pai Jih‐Tung , Weng Meng‐Shih 
Published: May 2017

ABSTRACTEpithelial‐to‐mesenchymal transition (EMT) is a major process to regulate cell migration and invasion. Inhibition of epidermal growth factor receptor (EGFR)‐mediated EMT by tyrosine kinase inhibitors (TKIs) is a strategy to prevent lung cancer invasion. However, drug resistance is emerged and accelerated invasion through other signaling bypassing EGFR after TKIs therapy. c‐Met signaling pathway is highly activated in EGFR‐mutated lung cancer cells. Targeting c‐Met signaling pathway may be a strategy to suppress EGFR‐independent migration and invasion for lung cancer therapy. Therefore, we examined the anti‐migration and anti‐invasion abilities of shikonin, an active compound from Lithospermum erythrorhizon, in highly and ligand‐induced c‐Met activation lung cancer cells. Our results revealed that cell viability and cell cycle progression did not change under 1 μM of shikoinin treatment in highly c‐Met expressive HCC827 lung cancer cells. Endogenous c‐Met activation was dose‐dependently inhibited and the migration and invasion activity of HCC827 cells were suppressed by shikonin treatment. Induction of E‐cadherin expression and inhibition of vimentin, slug, and snail expression by shikonin was through c‐Met‐mediated PI3K/Akt and ERK signaling suppression. Furthermore, hepatocyte growth factor (HGF)‐induced migration, invasion and EMT marker change were reversed by shikonin in low c‐Met expressive A549 lung cancer cells. Inhibition of HGF‐induced c‐Met, PI3K/Akt and MEK/ERK activation were observed in shikonin‐treated cells. Co‐treatment of PI3K/Akt inhibitor or ERK inhibitor with shikonin enhanced shikonin‐reversed HGF‐regulated EMT marker expression. Taken together, the results suggested that the anti‐migration and anti‐invasion activities of shikonin was through c‐Met inhibition and following by EMT suppression in lung cancer. J. Cell. Biochem. 118: 4639–4651, 2017. © 2017 Wiley Periodicals, Inc.
Decreased expression of hsa_circ_0003570 in hepatocellular carcinoma and its clinical significance
Authors: Fu Liyun , Wu Shengdong , Yao Ting , Chen Qingqing , Xie Yi , Ying Sheng , Chen Zhigang , Xiao Bingxiu , Hu Yaoren 
Published: May 2017

BackgroundCircular RNAs (circRNAs) constitute a class of non‐coding RNAs recently discovered to be widespread and abundant in mammalian cells. However, the expression features of most of circRNAs in hepatocellular carcinoma (HCC) are unraveled. In this study, we focused on hsa_circ_0003570, which was found to be down‐regulated in HCC tissues in our previous microarray screening.MethodsThe hsa_circ_0003570 levels in HCC cell lines, HepG2, SMMC‐7721, MHCC97L, MHCC97H, and HCCLM3, and human normal hepatic cell line L02 were detected by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Then, its levels in 107 paired HCC tissues and adjacent non‐tumor tissues, 60 liver biopsy samples from patients with chronic liver diseases were detected by qRT‐PCR. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of hsa_circ_0003570 for HCC.ResultsHsa_circ_0003570 was not only first found down‐regulated in HCC cell lines (P<.001) but also in HCC tissues (P<.001). Moreover, hsa_circ_0003570 was gradually decreased from chronic hepatitis (CH), to liver cirrhosis (LC) and to HCC tissues (P<.01). Its expression levels were significantly correlated with tumor diameter (P=.035), differentiation (P=.013), microvascular invasion (P=.045), Barcelona Clinic Liver Cancer stages (P=.011), tumor‐node‐metastasis stages (P=.016), and serum alpha‐fetoprotein levels (P=.031). The ROC curve demonstrated that hsa_circ_0003570 had poor performance for differentiating HCC from LC and CH, but had relatively good performance for differentiating LC from CH.ConclusionsThese results indicated that hsa_circ_0003570 expression levels were associated with HCC clinicopathological characteristics.
What would your neighbor do? An experimental approach to the study of informal social control of intimate partner violence in South Korea
Authors: Emery Clifton R. , Yang Hyerin , Kim Oksoo , Arenas Carmen , Astray Andres 
Published: May 2017

AbstractAlthough research on bystander intervention and informal social control of intimate partner violence (IPV) is increasingly common, empirical anomalies remain and experimental studies on population samples are rare. This study reports the effects of a new experimental approach to the study of informal social control of IPV by neighbors on a small population sample of 100 married men in Seoul, South Korea. We hypothesized that men randomly assigned to a high‐perceived informal social control condition would have lower self‐estimated likelihoods of IPV perpetration in response to a vignette. We also hypothesized that the effect of random assignment would be different for that portion of the sample that reported perpetration of family violence (IPV or child abuse). Compared to the nonperpetrating portion of the sample, perpetrators of family violence in the sample randomly assigned to the high perceived control condition experienced a significant drop in self‐estimated likelihood of IPV perpetration.
Removal of arsenic (III) and arsenic (V) from aqueous solutions through adsorption by Fe/Cu nanoparticles
Authors: Babaee Yassaman , Mulligan Catherine N , Rahaman Md Saifur 
Published: May 2017

AbstractBACKGROUNDWhile various iron‐based nanomaterials have been studied for the removal of arsenic from groundwater or its immobilization in soils, this study focuses on the applicability of iron/copper bimetallic nanoparticles for removal of arsenic from synthetic contaminated waters. In order to determine the effectiveness of these nanoparticles for arsenic removal, after synthesis, various sorption tests were performed with aqueous arsenic solutions.RESULTSDetailed physicochemical characterization of synthesized nanoparticles confirmed the successful formation of Fe/Cu nanoparticles with a mean diameter of 13.17 nm. These nanoparticles were found to be effective for removing arsenic from aqueous solutions. The maximum sorption capacities for As(III) and As(V) were 19.68 mg g−1 and 21.32 mg g−1, respectively, at a pH of 7.0. Adsorption isotherms fit well into the Langmuir equation, and sorption follows pseudo‐second‐order kinetics. Coexisting carbonate, sulfate, and phosphate ions had no significant effect on the removal efficiency of arsenic at the concentrations studied. Arsenic removal efficiency by Fe/Cu nanoparticles is enhanced in acidic environments and in basic conditions, desorption of arsenic is possible.CONCLUSIONThe Fe/Cu nanoparticle powder was found to be effective for removal of arsenic from water and has potential to be used for arsenic remediation from the aquatic environment or in situ immobilization of arsenic. © 2017 Society of Chemical Industry
Sonographic findings of IgG4‐related disease of the salivary glands: Case report and review of the literature
Authors: Narayan Anand K. , Baer Alan , Fradin Joel 
Published: May 2017

ABSTRACTIgG4‐related disease is a relatively newly described entity with findings that historically have been confused with a variety of other diseases with markedly different treatments and prognoses, including Sjogren's syndrome and MALT lymphoma. We present a case of IgG4‐related disease of the salivary glands and review the literature on sonographic findings of IgG4‐related disease. By connecting sonographic features of IgG4 disease with common ancillary features of IgG4 disease in other sites (pancreas, hilar lymph nodes, biliary tree, thyroid, and retroperitoneum), radiologists can play a pivotal role in the early diagnosis and identification of IgG4‐related disease. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 46:73–77, 2018;
Electrothermal formulation of the partial element equivalent circuit method
Authors: Lombardi Luigi , Raimondo Raffaele , Antonini Giulio 
Published: May 2017

AbstractThis paper presents a combined electrothermal formulation of the partial element equivalent circuit (PEEC) method. It is shown that the distributed thermal problem can be formulated as an equivalent thermal network that shares the same topological and geometrical properties of the PEEC equivalent model. Temperature‐dependent thermal heat conduction and convection models are adopted thus leading to a time‐varying thermal network, which is studied by using pertinent techniques along with the electrical equivalent circuit. The proposed electrothermal PEEC solver is used to analyze 2 problems of interest and verified through comparison with a commercial software.
Assessment of thermal damage in total knee arthroplasty using an osteocyte injury model
Authors: Kurata Kosaku , Matsushita Junpei , Furuno Atsushi , Fujino Junichi , Takamatsu Hiroshi 
Published: May 2017

ABSTRACTPolymethylmethacrylate bone cement has been widely used for the anchorage of artificial implants in various orthopedic surgeries. Although it is one of the most successful biomaterials in use, excess heat generation intrinsically causes thermal damage to bone cells adjacent to the bone cement. To estimate a risk of thermal injury, a response of bone cells to cement polymerization must be elucidated because of the occurrence of thermal damage. Thermal damage is affected not only by maximal temperature but also by exposure time, temperature history, and cell type. This study aimed at quantifying the thermal tolerance of bone cells for the development of a thermal injury model, and applying this model for the estimation of thermal damage during cement polymerization in total knee arthroplasty. Osteocytes, osteoblasts, and fibroblasts were respectively subjected to steady supraphysiological temperatures ranging from 45 to 50°C. Survival curves of each cell and temperatures were used to formulate the Arrhenius model. A three‐dimensional heat conduction analysis for total knee arthroplasty was conducted using the finite element model based on serial CT images of human knee. A maximal temperature rise of 50°C was observed at the interface between the 3‐mm thick cement and the tissue immediately beneath the tibial tray of the prosthesis. The probability of thermal damage to the osteocyte, which was calculated using the Arrhenius model, was negligible at a distance of at least 1 mm away from the cement–bone interface. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2799–2807, 2017.
Effect of impact assembly on the interface deformation and fretting corrosion of modular hip tapers: An in vitro study
Authors: Panagiotidou Anna , Cobb Timothy , Meswania Jay , Skinner John , Hart Alister , Haddad Fares , Blunn Gordon 
Published: May 2017

ABSTRACTWear and corrosion at the modular head–neck junction has been recognised to be a potential clinical concern, with multiple reports on adverse local tissue reactions and subsequent early failure of metal‐on‐metal hip replacements. Furthermore, reports on head–neck taper corrosion are also being described with conventional metal‐on‐polyethylene bearings. Manufacturing tolerances, surgical technique, non‐axial alignment, material combination, high frictional torque and high bending moment have all been implicated in the failure process. There is limited guidance on the force of impaction with which surgeons should assemble modular hip prostheses. This study aims to investigate the effect of impaction force on the deformation and corrosion of modular tapers. Short neck tapers with high surface roughness (average Rz = 16.58 μm, Ra = 4.14μm) and long neck tapers with low surface roughness (average Rz = 3.82 μm, Ra = 0.81μm), were assembled with CoCrMo alloy heads (smooth finish) under controlled conditions with 2, 4 or 8 kN of impaction force. Material combinations tested included CoCrMo‐head/CoCrMo‐neck and CoCrMo‐head/Ti‐6Al‐4V‐neck. Assessment of surface deformation before and after impaction was made using surface profilometry. Measurement of fretting current during sinusoidal cyclic loading evaluated mechanically assisted corrosion for each assembly load during short‐term cyclic loading (1000‐cycles) and long‐term cyclic loading (5 million‐cycles). Deformation on head and neck tapers increased with assembly load. Fretting currents during short term simulation testing showed significantly lower currents (p < 0.05), in 8 kN assemblies when compared to 2 and 4 kN, especially for the short‐rough tapers. Long‐term simulator testing demonstrated a progressive reduction in fretting corrosion for samples impacted with 4 and 8 kN; however, this reduction was greater for samples impacted at 8 kN even at the start of testing. Based on our results, surgeons could minimise mechanically assisted crevice corrosion by using higher impact loads when assembling the head to the stem in total hip arthroplasty. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:405–416, 2018.
Metabolomics approach based on ultra‐high‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry to identify the chemical constituents of the Traditional Chinese Er‐Zhi‐Pill
Authors: Zhong Xunlong , Zhong Yanmei , Yan Kangqi , Xiao Xuerong , Duan Lian , Wang Ruolun , Wang Laiyou 
Published: May 2017

Er‐Zhi‐Pill, which consists of Ligustri lucidi fructus and Ecliptae prostratae herba, is a classical traditional Chinese medicinal formulation widely used as a liver‐nourishing and kidney‐enriching tonic. To identify the bioactive ingredients of Er‐Zhi‐Pill and characterize the variation of chemical constituents between co‐decoction and mix of individually decocted L. lucidi fructus and E. prostratae herba, a novel metabolomics approach based on ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry in both positive and negative ion modes, was established to comprehensively analyze chemical constituents and probe distinguishable chemical markers. In total, 68 constituents were unambiguously or tentatively identified through alignment of accurate molecular weights within an error margin of 5 ppm, elemental composition and fragmentation characteristics, including eight constituents, which were confirmed by comparing to reference standards. Furthermore, principal component analysis and partial least squares discriminant analysis using Simca‐p+ 12.0 software were applied to investigate chemical differences between formulations obtained by co‐decoction and a mixture of individual decoctions. Global chemical differences were found in samples of two different decoction methods, and 16 components, including salidroside, specneuzhenide and wedelolactone, contributed most to the observed differences. This study provides a basic chemical profile for the quality control and further mechanism research of Er‐Zhi‐Pill.
Enantioselective analysis of bambuterol in human plasma using microwave‐assisted chiral derivatization coupled with ultra high performance liquid chromatography and tandem mass spectrometry
Authors: Zhou Ting , Zeng Jing , Zhao Ting , Zhong Qisheng , Yang Yang , Tan Wen 
Published: May 2017

In this study, an enantioselective analytical method based on microwave‐assisted chiral derivatization coupled with ultra high performance liquid chromatography and tandem mass spectrometry was developed for the determination of bambuterol enantiomers in human plasma. The chiral derivatization reaction was greatly accelerated by microwave irradiation. Under the optimized conditions, both the derivatization time and separation time on column was only 3 min, and the lower limit of quantification was 2.5 pg/mL. The recoveries were in the range of 90.1–93.0% without significant matrix effect. Compared with the conventional heating chiral derivatization, microwave‐assisted chiral derivatization obtained higher chiral derivatization yields with much shorter time due to the effect of microwave irradiation. Furthermore, the racemization during the derivatization reaction was systematically investigated. The results showed the concentration of acetic acid and the reaction time had significant effects on the racemization, which could be well controlled during microwave‐assisted chiral derivatization for the short reaction time. Finally, this novel approach was demonstrated by determining bambuterol in human plasma of a clinical pharmacokinetic study in eight healthy volunteers. On the basis of the results, microwave‐assisted chiral derivatization coupled with ultra high performance liquid chromatography and tandem mass spectrometry as a simple and effective enantioselective analysis technique for the determination of chiral drugs in complex biological samples showed great promise.