bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells
Authors: Hidayat Meilinah , Prahastuti Sijani , Fauziah Nurul , Maesaroh Maesaroh , Balqis Balqis , Widowati Wahyu 
Published: July 2016

<p class="Abstract">In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.</p><p><strong>Video Clip:</strong></p><p><a href="https://youtube.com/v/UWv9nChmF2Y">Maintaining 3T3-L1 cell culture</a>: 5 min 15 sec</p>
Complications of porous-coated press-fit cementless total hip replacement in dogs
Authors: Kidd Scott W. , Preston Christopher A. , Moore George E. 
Published: July 2016

SummaryObjective: To report postoperative complications using a commercially available porous-coated press-fit cementless total hip replacement (THR) system in dogs.Methods: Medical records were reviewed for client-owned dogs with hip pathologies requiring THR. A minimum of six-week postoperative orthopaedic examination and orthogonal pelvic radiographs were used to assess outcome and complications in the perioperative period. Referring veterinarian medical records, phone interviews with clients, or both were used to assess long-term functional outcome and complications.Results: Bilateral THR was performed in 36 dogs, and unilateral in 147 dogs, making a total of 219 THR procedures in 183 dogs. A total complication rate of 31.1% (68/219) was observed. A catastrophic complication was observed in 8.2% (n = 18), a major complication in 9.6% (n = 21), and a minor complication in 13.2% (n = 29) of procedures. The most common complications were intra-operative femoral fissure (n = 46), diaphyseal femoral fracture (n = 15), and coxofemoral luxation (n = 9). Full return to function was achieved in 88.1% of procedures with a median follow-up period of 42 months.Clinical significance: Porous-coated press-fit cementless collarless total hip replacements have a high complication rate. The majority of complications occur intra-operatively or perioperatively, with few complications occurring beyond 12 weeks postoperatively. Both fissure fractures and diaphyseal femoral fractures carry a favourable prognosis with immediate cerclage wiring and plate fixation, respectively.
GEOMETRY AND CHARACTERISTICS OF THE HELIOSHEATH REVEALED IN THE FIRST FIVE YEARS OF INTERSTELLAR BOUNDARY EXPLORER OBSERVATIONS
Authors: Zirnstein E. J. , Funsten H. O. , Heerikhuisen J. , McComas D. J. , Schwadron N. A. , Zank G. P. 
Published: July 2016

ABSTRACT
We investigate and interpret the geometry and characteristics of the inner heliosheath (IHS) plasma and their impact on the heliotail structure as observed in energetic neutral atom (ENA) maps acquired during the first 5 yr of Interstellar Boundary Explorer (IBEX) observations. In particular, IBEX observations of the heliotail reveal distinct, localized emission features (lobes) that provide a rich set of information about the properties and evolution of the heliosheath plasma downstream of the termination shock (TS). We analyze the geometry of the heliotail lobes and find that the plane intersecting the port and starboard heliotail lobe centers is ∼6° from the solar equatorial plane, and the plane intersecting the north and south heliotail lobe centers is ∼90° from the solar equatorial plane, both indicating strong correlation with the fast–slow solar wind asymmetry, and thus reflecting the structure of the IHS flow around the Sun. We also analyze the key parameters and processes that form and shape the port and starboard lobes, which are distinctly different from the north and south lobes. By comparing IBEX ENA observations with results from a simplistic flow model of the heliosphere and a multicomponent description for pickup ions (PUIs) in the IHS, we find that the port and starboard lobe formation is driven by a thin IHS, large nose–tail asymmetry of the distance to the TS (and consequently, a large nose–tail asymmetry of the relative abundance of PUIs at the TS) and the energy-dependent removal of PUIs by charge exchange in the IHS.
TRACING THE CHROMOSPHERIC AND CORONAL MAGNETIC FIELD WITH AIA, IRIS, IBIS, AND ROSA DATA
Authors: Aschwanden Markus J. , Reardon Kevin , Jess Dave B. 
Published: July 2016

ABSTRACT
The aim of this study is to explore the suitability of chromospheric images for magnetic modeling of active regions. We use high-resolution images (

), from the Interferometric Bidimensional Spectrometer in the Ca ii 8542 Å line, the Rapid Oscillations in the Solar Atmosphere instrument in the Hα 6563 Å line, the Interface Region Imaging Spectrograph in the 2796 Å line, and compare non-potential magnetic field models obtained from those chromospheric images with those obtained from images of the Atmospheric Imaging Assembly in coronal (171 Å, etc.) and in chromospheric (304 Å) wavelengths. Curvi-linear structures are automatically traced in those images with the OCCULT-2 code, to which we forward-fitted magnetic field lines computed with the Vertical-current Approximation Nonlinear Force Free Field code. We find that the chromospheric images: (1) reveal crisp curvi-linear structures (fibrils, loop segments, spicules) that are extremely well-suited for constraining magnetic modeling; (2) that these curvi-linear structures are field-aligned with the best-fit solution by a median misalignment angle of

–7°; (3) the free energy computed from coronal data may underestimate that obtained from cromospheric data by a factor of

–4, (4) the height range of chromospheric features is confined to

km, while coronal features are detected up to h = 35,000 km; and (5) the plasma-β parameter is

for all traced features. We conclude that chromospheric images reveal important magnetic structures that are complementary to coronal images and need to be included in comprehensive magnetic field models, something that is currently not accomodated in standard NLFFF codes.
Manipulating Memory CD8 T Cell Numbers by Timed Enhancement of IL-2 Signals
Authors: Kim Marie T. , Kurup Samarchith P. , Starbeck-Miller Gabriel R. , Harty John T. 
Published: July 2016

Abstract
As a result of the growing burden of tumors and chronic infections, manipulating CD8 T cell responses for clinical use has become an important goal for immunologists. In this article, we show that dendritic cell (DC) immunization coupled with relatively early (days 1–3) or late (days 4–6) administration of enhanced IL-2 signals increase peak effector CD8 T cell numbers, but only early IL-2 signals enhance memory numbers. IL-2 signals delivered at relatively late time points drive terminal differentiation and marked Bim-mediated contraction and do not increase memory T cell numbers. In contrast, early IL-2 signals induce effector cell metabolic profiles that are more conducive to memory formation. Of note, downregulation of CD80 and CD86 was observed on DCs in vivo following early IL-2 treatment. Mechanistically, early IL-2 treatment enhanced CTLA-4 expression on regulatory T cells, and CTLA-4 blockade alongside IL-2 treatment in vivo prevented the decrease in CD80 and CD86, supporting a cell-extrinsic role for CTLA-4 in downregulating B7 ligand expression on DCs. Finally, DC immunization followed by early IL-2 treatment and anti–CTLA-4 blockade resulted in lower memory CD8 T cell numbers compared with the DC+early IL-2 treatment group. These data suggest that curtailed signaling through the B7-CD28 costimulatory axis during CD8 T cell activation limits terminal differentiation and preserves memory CD8 T cell formation; thus, it should be considered in future T cell–vaccination strategies.
A Simple, Versatile Antibody-Based Barcoding Method for Flow Cytometry
Authors: Akkaya Billur , Miozzo Pietro , Holstein Amanda H. , Shevach Ethan M. , Pierce Susan K. , Akkaya Munir 
Published: July 2016

Abstract
Barcoding of biological samples is a commonly used strategy to mark or identify individuals within a complex mixture. However, cell barcoding has not yet found wide use in flow cytometry that would benefit greatly from the ability to analyze pooled experimental samples simultaneously. This is due, in part, to technical and practical limitations of current fluorescent dye-based methods. In this study, we describe a simple, versatile barcoding strategy that relies on combinations of a single Ab conjugated to different fluorochromes and thus in principle can be integrated into any flow cytometry application. To demonstrate the efficacy of the approach, we describe the results of a variety of experiments using live cells as well as fixed and permeabilized cells. The results of these studies show that Ab-based barcoding provides a simple, practical method for identifying cells from individual samples pooled for analysis by flow cytometry that has broad applications in immunological research.
Fast Solution of Three-Dimensional Modified Helmholtz Equations by the Method of Fundamental Solutions
Authors: Lin Ji , Chen C. S. , Liu Chein-Shan 
Published: July 2016

AbstractThis paper describes an application of the recently developed sparse scheme of the method of fundamental solutions (MFS) for the simulation of three-dimensional modified Helmholtz problems. The solution to the given problems is approximated by a two-step strategy which consists of evaluating the particular solution and the homogeneous solution. The homogeneous solution is approximated by the traditional MFS. The original dense system of the MFS formulation is condensed into a sparse system based on the exponential decay of the fundamental solutions. Hence, the homogeneous solution can be efficiently obtained. The method of particular solutions with polyharmonic spline radial basis functions and the localized method of approximate particular solutions in combination with the Gaussian radial basis function are employed to approximate the particular solution. Three numerical examples including a near singular problem are presented to show the simplicity and effectiveness of this approach.
Direct Calculation of Permeability by High-Accurate Finite Difference and Numerical Integration Methods
Authors: Wang Yi , Sun Shuyu 
Published: July 2016

AbstractVelocity of fluid flow in underground porous media is 6~12 orders of magnitudes lower than that in pipelines. If numerical errors are not carefully controlled in this kind of simulations, high distortion of the final results may occur [1–4]. To fit the high accuracy demands of fluid flow simulations in porous media, traditional finite difference methods and numerical integration methods are discussed and corresponding high-accurate methods are developed. When applied to the direct calculation of full-tensor permeability for underground flow, the high-accurate finite difference method is confirmed to have numerical error as low as 10–5% while the high-accurate numerical integration method has numerical error around 0%. Thus, the approach combining the high-accurate finite difference and numerical integration methods is a reliable way to efficiently determine the characteristics of general full-tensor permeability such as maximum and minimum permeability components, principal direction and anisotropic ratio.
Immediate and Delayed Effects of Diode Laser on Debonding of Ceramic Brackets: An in vitro Study
Authors: Rajvikram N , Rajakumar P , Saravanan R , Anand Praveena , Anand PB , Prabhakar Ramachandran , Atali Varun Reddy 
Published: July 2016

ABSTRACT

Aim
The aim of the study is to evaluate the immediate and delayed effects of diode laser on debonding of ceramic brackets.

Materials and methods
A total of 60 human extracted premolar teeth were randomly assigned to three different treatment groups. All teeth were bonded with adhesive precoated (APC) ceramic brackets (3M Unitek). A total of 20 teeth were debonded without lasing (group 1), 20 immediately after lasing (group 2), and 20 1 hour after lasing (group 3). For the lasing groups (groups 2 and 3), access cavity was prepared on the occlusal surface to a 2 mm diameter. A transbond plus self-etching primer (3M Unitek, Monrovia, CA, USA) and APC PLUS clarity advanced brackets (3M, Unitek, Monrovia, CA, USA) were used. The shear bond strength (SBS) and adhesive remnant index (ARI) were measured. The internal pulpal wall temperature was noted for the laser groups.

Results
The mean SBS was 15.4, 11.57, and 11.79 MPa for groups 1 to 3 respectively. Post hoc test showed significant difference (p < 0.001) between the control group and the lased groups. For groups 2 and 3, the rise in temperature was at an average of 1.4 and 1.3°C respectively.

Conclusion
The SBS of APC brackets decreased by 33.3% on application of diode laser without increasing the internal pulp chamber wall temperature significantly. Shear bond strength remains more or less the same whether debonding is done immediately after lasing or 1 hour after lasing. Diode lasers increased the ARI scores and thus decreased the risk of enamel fracture.

How to cite this article
Anand P, Anand PB, Prabhakar R, Rajvikram N, Rajakumar P, Atali VR, Saravanan R. Immediate and Delayed Effects of Diode Laser on Debonding of Ceramic Brackets: An in vitro Study. J Contemp Dent Pract 2016;17(4):275-281.
Chemical and Topographic Analysis of Eight commercially Available Dental Implants
Authors: Peck Mogammad Thabit , Chrcanovic Bruno R 
Published: July 2016

ABSTRACT

Background
Surface characterization of dental implants allows us to better understand the effects of the implant on the host biological response. In this study, we analyzed and compared these characteristics among implants commercially available in South Africa.

Materials and methods
Eight implants from different manufacturers were chosen for analysis (Touareg, ICE, (R)Evolutions, Uniti, AnyRidge, MIS, Ivory-QSI, Southern), using scanning electron microscopy (SEM), interferometry, and energy dispersive X-ray spectroscopy to study the surface chemical composition and morphology.

Results
The results indicate that variations in manufacturer processes result in implant surfaces that are distinctly different from one another. Most implants presented a moderately rough surface with sandblasted-only implant surfaces having a lower mean value of Sa when compared with sandblasted and acidetched surfaces. Carbon contamination was detected on all the implants and that of aluminum on five implant surfaces. Ca and P were detected on the surface of Touareg implants, indicating the manufacturer's attempt to enhance osseointegration.

Conclusion
The surface of the implants showed a range of chemical, physical properties, and surface topographies.

Clinical significance
The results indicate that implant surface treatment is not standardized. This may have clinical implications. Further clinical research is required.

How to cite this article
Peck MT, Chrcanovic BR. Chemical and Topographic Analysis of Eight commercially Available Dental Implants. J Contemp Dent Pract 2016;17(5):354-360.
Sense of Coherence and Oral Health Status among 16 to 17-year-old Preuniversity Students of Virajpet Taluk: A Cross-sectional Study
Authors: Shilpa M , Naik Sandhya P , Potdar Shrudha , Reddy Siddana G , Patwardhan Priyanka K , Shree Swetha S 
Published: July 2016

ABSTRACT

Background
Sense of coherence (SOC) is an important psychological factor that enables people to cope with stressors and successfully maintain and improve health. Oral health affects general health and impacts quality of life. The aim of the present study is to assess the association between SOC and oral health status among 16 to 17-year-old preuniversity students in Virajpet, Karnataka, India.

Materials and methods
A total of 361 subjects were included in the study by stratified cluster random sampling method. Sense of coherence was measured using the Kannadatranslated 13-item scale proposed by Antonovsky. Oral health status was measured using oral hygiene index – simplified (OHI-S), Silness and Loe Plaque index, Community Periodontal Index (CPI) and Loss of Attachment (LA) as well as decayedmissing- filled teeth (DMFT) index. Chi-square test and analysis of variance were used to evaluate the association between SOC and oral health.

Results
There was no statistically significant association of plaque index and caries experience with SOC, whereas the SOC scores were found to be significantly associated with loss of attachment and OHI-S among the subjects. The distribution of the mean number of sextants with loss of attachment was more among males.

Conclusion
Sense of coherence has a role to play in the maintenance of oral health. There is a need to closely examine the psychodynamic factors that affect oral health.

Clinical significance
There is good evidence that SOC is a resource capable of promoting health, strengthening resilience, and developing a subjectively positive state of health.

How to cite this article
Shilpa M, Naik SP, Potdar S, Reddy SG, Patwardhan PK, Shree SS. Sense of Coherence and Oral Health Status among 16 to 17-year-old Preuniversity Students of Virajpet Taluk: A Cross-sectional Study. J Contemp Dent Pract 2016;17(5):388-393.
Zirconia Crown as Single Unit Tooth Restoration: A Literature Review
Authors: Alfawaz Yasser 
Published: July 2016

ABSTRACT
Ceramics has become increasingly popular as a dental restorative material because of its superior esthetics, as well as its inertness and biocompatibility. Among dental ceramics, zirconia is used as a dental biomaterial and it is the material of choice in contemporary restorative dentistry. Zirconia ceramics has both clinical popularity and success due to its outstanding mechanical properties and ease of machining in the green stage via computer-aided design and computer-aided manufacturing technology. Zirconia is one of the most promising restorative biomaterial because it has favorable mechanical and chemical properties suitable for medical application. Zirconia ceramics is becoming a prevalent biomaterial in dentistry. Clinical evaluations also indicate a good success rate for zirconia with minimal complications. This article reviews the current literature on dental zirconia with respect to basic properties, biocompatibility, and clinical applications in aesthetic dentistry as single unit crown.

How to cite this article
Alfawaz Y. Zirconia Crown as Single Unit Tooth Restoration: A Literature Review. J Contemp Dent Pract 2016;17(5):418-422.
Influence of proxy data uncertainty on data assimilation for the past climate
Authors: Matsikaris Anastasios , Widmann Martin , Jungclaus Johann 
Published: July 2016

Abstract. Data assimilation (DA) is an emerging topic in palaeoclimatology and one of the key challenges in this field. Assimilating proxy-based continental mean temperature reconstructions into the MPI-ESM model showed a lack of information propagation to small spatial scales . Here, we investigate whether this lack of regional skill is due to the methodology or to errors in the assimilated reconstructions. Error separation is fundamental, as it can lead to improvements in DA methods. We address the question by performing a new set of simulations, using two different sets of target data; the proxy-based PAGES 2K reconstructions (DA-P scheme), and the HadCRUT3v instrumental observations (DA-I scheme). Again, we employ ensemble-member selection DA using the MPI-ESM model, and assimilate Northern Hemisphere (NH) continental mean temperatures; the simulated period is 1850–1949 AD. Both DA schemes follow the large-scale target and observed climate variations well, but the assimilation of instrumental data improves the performance. This improvement cannot be seen for Asia, where the limited instrumental coverage leads to errors in the target data and low skill for the DA-I scheme. No skill on small spatial scales is found for either of the two DA schemes, demonstrating that errors in the assimilated data are not the main reason for the unrealistic representation of the regional temperature variability in Europe and the NH. It can thus be concluded that assimilating continental mean temperatures is not ideal for providing skill on small spatial scales.
Automatic segmentation and classification of seven-segment display digits on auroral images
Authors: Savolainen Tuomas , Whiter Daniel Keith , Partamies Noora 
Published: July 2016

Abstract. In this paper we describe a new and fully automatic method for segmenting and classifying digits in seven-segment displays. The method is applied to a dataset consisting of about 7 million auroral all-sky images taken during the time period of 1973–1997 at camera stations centred around Sodankylä observatory in northern Finland. In each image there is a clock display for the date and time together with the reflection of the whole night sky through a spherical mirror. The digitised film images of the night sky contain valuable scientific information but are impractical to use without an automatic method for extracting the date–time from the display. We describe the implementation and the results of such a method in detail in this paper.
Reducing the effect of pre-slaughter fasting on the stress response of rainbow trout (Oncorhynchus mykiss)
Authors: Bermejo-Poza R , De la Fuente J , Pérez C , Lauzurica S , de Chávarri E González , Diaz MT , Villarroel M 
Published: July 2016

AbstractFasting is commonly used in aquaculture to empty the gut before slaughter, but little is known about how feeding frequency before fasting affects the stress response of trout. To find out more, 240 rainbow trout (Oncorhynchus mykiss) were separated into three groups with different feeding schedules during the final month of fattening, from 26 September to 28 October 2013 (daily, every two days or every four days) and two durations of pre-slaughter fasting (two days of fasting; 24.3 degree days, to nine days of fasting; 102 degree days). After slaughter, a number of stress-related parameters were measured, such as liver glycogen, skin/gill colour and haematological parameters (cortisol, glucose, lactate, triglycerides, lactate dehydrogenase and creatine phosphokinase). Trout given food every two days on the farm had lower levels of cortisol and higher levels of triglycerides and liver glycogen than the other treatments after two days of fasting; indicating that habituating trout to feed once every two days in the final month of fattening lowered their stress response to two days of fasting before slaughter.