bluuebunny/crossref_metadata_2025_split huggingface dataset:
ВПЛИВ КЛОЗАВЕРМУ А ТА КАТОЗАЛУ НА АНТИОКСИДАНТНИЙ СТАТУС ОРГАНІЗМУ КОРІВ ЗА ЕКСПЕРИМЕНТАЛЬНОГО ФАСЦІОЛЬОЗУ, СЕНСИБІЛІЗОВАНИХ АТИПОВИМИ МІКОБАКТЕРІЯМИ
Authors: Kuljaba O.V. , Stybel V.V. , Gutyj B.V. 
Published: October 2016

The article deals with the results of researches of fasciolosis and myco bacteriosis on the activity of enzymatic antioxidant system level and the level of lipid peroxidation products.Fasciola and mycobacteria inhibit the activity of enzymes of antioxidant defense system in the liver hosts, as indicated by decreased activity of catolase by 23%, superoxide dismutase by 35% and increase of lipid hydroperoxides level by 43% (p < 0.001), TBA-products by 23% (p < 0.001) compared with healthy animals.The use of clozaverm A and catozale by research cows promotes the reducing of HPL and TBA- active products in their blood, preventing the development of oxidative stress. Thus, the use of clozaverm A and catozale by animal from the research groups contributed to rapid increase of catolase activity, beginning with the 7th day of the experiment. At the 21st and 28th day of experiment the activity of investigated enzyme varies within the limits of physiological values. At the same time in a certain relationship with the intensity of redox processes in the tissues of animals is SOD activity. At the 21st day of the experiment enzyme activity in experimental group D2 was increased by 34% compared to the control group.The analysis of the obtained results indicates that superoxide dismutase activity in serum of cows of the research groups has positively correlated action with the activity of catolase.After application of clozaverm A for the treatment of cows by the experimental fasciolosis, sensitized atypical mycobacteria, is set the decrease in the intensity of lipid per oxidation at the 14th day of the experiment HPL by 11%, and the level of TBA-active products, respectively, by 9% compared to control.The inhibition of lipid per oxidation for treatment of animal with drugs «Clozaverm A» and «Catozal» caused by activation of metabolic processes in the organism, which involve enzymes, including enzymes and antioxidants that catalyze oxidation and phosphorylation, and also by strengthening erytro poetical function of bone marrow.
БІОЛОГІЧНЕ ЗНАЧЕННЯ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ ОРГАНІЗМУ ТВАРИН
Authors: Lavryshyn Y.Y. , Varkholyak I.S. , Martyschuk T.V. , Guta Z.А. , Ivankiv L.B. 
Published: October 2016

In the review of the literature it was generalized the data due to the classification and characterization of antioxidant protection system of animals body. This model combines a number of different by its nature substances. Each of the components of the antioxidant system operates in close relationship with its other structural elements, harmoniously, and in many cases complements and in many cases - enhances the action of each other. Glutathione system forms functional basis of antioxidant defense system, constituent elements of which has its own glutathione and enzymes, which catalyze the reaction of its reverse transformation (oxidation ↔ recovery). Glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase are attributed to these enzymes.Most researchers conventionally distributed antioxidant defense system in enzyme and non-enzyme. Catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione transferase and other enzymes are included to enzymatic link of antioxidant defense system. Fat-soluble vitamins A, E and K, water-soluble vitamins C and PP, biogenic amines, glutathione, carotenoids, ubiquinone, sterols are included to non-enzyme system. As the enzyme, as non-enzyme antioxidant defense system is present in the bloodstream. The activity of enzymatic antioxidant system is well regulated and depends on the age of the animals, physiological condition, the dynamics of hormone, synthesis intensity of antioxidant enzyme, pH medium, the presence of coenzymes, inhibitors, activators, and other factors. Non-enzyme link of antioxidant system does not need so many regulators as the most chemical substance - antioxidant - enters into chemical reaction with the radical. The rate of reaction may be only changed.
ПОРІВНЯЛЬНА ЕФЕКТИВНІСТЬ ЛІКУВАЛЬНО–ПРОФІЛАКТИЧНИХ ПРЕПАРАТІВ ПРИ ЗАСТОСУВАННІ 3% СПИРТОВО–ВОДНОЇ ЕМУЛЬСІЇ ПРОПОЛІСУ ТА АНТИБІОТИКІВ ЗА КАТАРАЛЬНОЇ БРОНХОПНЕВМОНІЇ ТЕЛЯТ
Authors: Levkivska N. , Gutyj B.V. , Levkivskyj D. 
Published: October 2016

Catarrhal bronchopneumonia of calves appears on the background of adverse environmental factors, actions conditionally pathogenic microflora particularly Str.pneumoniae and their associations that cause immune deficiency of animals. Experimental research was carried out in the agricultural firm Ltd. «Pravda» Brody district, Lviv region. For research it was formed three groups of calves: first – control (healthy calves 5 goals); second and third groups – research (calves suffering from catarrhal bronchopneumonia caused by the causative agent Str. pneumoniae – 7 goals), as well as the a third research group – 7 goals, diseases caused by microorganisms association (isolated from nasal exudate).Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli. were isolated from the nasal passages of calves on catarrhal bronchopneumonia.Materials of antibiotic gramme showed the highest sensitivity cultures of bacteria isolated from nasal fluid holes of calves suffering from catarrhal bronchopneumonia to 3% of ethanol–water emulsion of propolis.The use of aerosols to 3% of ethanol–water emulsion of propolis with medicinal purposes for catarrhal bronchopneumonia of calves, 1 time a day, during 6 days contributed the stimulation of cellular and humoral factors of calves body protection and their recovery. After use of aerosols – 3% of ethanol–water emulsion of propolis in calves suffering from catarrhal bronchopneumonia, of both research groups in serum was increased the content: in calves of the first group Ig G to 8.18 mg/ml, Ig M to 0.7 mg/ml, and second, respectively, 6.85 mg/ml and 0.3 mg/ml.It was noted a level decrease of CIC 1.6 times compared with the data of sick animals. The use of 3% alcohol–water emulsion of propolis to the calves with catarrhal bronchopneumonia contributed to increased activity of bactericidal and lizocimic activity whereby these figures were higher in the first experimental group of calves, in which Str. pneumoniae was the causative agent, compared with calves from the second research group. Prophylactic use of aerosols of 3% ethanol – water emulsion of propolis prevented disease in calves with catarrhal bronchopneumonia.
ФЕНОТИПОВІ ТА МОРФОЛОГІЧНІ ЗМІНИ КУЛЬТУРИ КЛІТИН КІСТКОВОГО МОЗКУ ЩУРІВ В ПРОЦЕСІ ЇХ КУЛЬТИВУВАННЯ
Authors: Mazurkiewicz A.I. , Kovpak V.V. , Kovpak O.S. 
Published: October 2016

Bone marrow is the only adult tissue which normally consists of immature undifferentiated and low differentiated cells which called stem cells and they are similar in structure to embryonic stem cells. But literature data analysis doesn't give an unambiguous answer regarding phenotypic and morphological changes of bone marrow cells culture of rats during their in vitro cultivation which necessitated further research.Investigate phenotypic and morphological changes of bone marrow cells culture of rats during their in vitro cultivation from first to fourth passage.We were used in these research bone marrow cells of rats from the first to the fourth passages. Microscopic analysis and evaluation morphological changes of bone marrow cells culture of rats during cultivation were carried out using inverted microscope Axiovert 40. Control of changes phenotype was performed by detecting CD markers (CD10, CD38, CD34, CD45, CD48, CD54, CD56, CD66e, CD96, CD227, CD326, pan–keratin). The evaluation was performed by the semi– quantitative method (H–Score).The research of primary culture of rat bone marrow cells showed that it morphologically heterogeneous, noted the small number of cells polygonal shape, surrounded by the fibroblast cells. During the cultivation cell culture becomes more homogenous at the expense of fibroblast–like cells. As a result of occurred the transition process from heterogeneous culture in zero passage to the most homogeneous culture in 4 passage. Immunophenotyping population of cell culture derived from rat bone marrow, revealed a high level of expression of pan–keratin; moderate level – CD34, CD48, CD66e, CD95; low level – CD38, CD45, CD56, CD227, CD326; lack of expression – CD10, CD54. Change of the expression of surface markers varies in each passage CD48, CD66e, CD95 increased significantly; CD38, SD45, SD326, pan–keratin reduced significantly. The markers CD34, CD 56, CD 227 were expressed on the one level from the first to the fourth passage. The expression of the CD10, CD54 markers during the study period was not identified.
КОРОЗІЙНА ДІЯ МИЙНО–ДЕЗІНФІКУЮЧОГО ЗАСОБУ «САН–АКТИВ» НА МЕТАЛЕВІ ПОВЕРХНІ ТЕХНОЛОГІЧНОГО ОБЛАДНАННЯ М’ЯСОПЕРЕРОБНОЇ ГАЛУЗІ
Authors: Salata V.Z. 
Published: October 2016

It was developed detergent–sanitizer «San active» for the sanitization of technology equipment for meat processing plants. Active substance of detergent–disinfectant «San active» is the TIME, STEAM, alkali, complexones and corrosion inhibitors. The importance of product development «San active» is that it combines two important features – a washing and disinfection.Previously it was established that means «San active» has good washing properties and shows high bactericidal effect on gram–positive and gram–negative microflora. Bactericidal action of «San active» at 14,8 times is more active compared with phenol, and in the presence in the environment the action of protein substances its activity decreases in 2,3 – 2,4 times. Remedy of 0,5% concentration has bactericidal effect on cells of S. aureus and E. faecalis, which are in the biofilm after 10 minutes of exposure. For the inactivation of cells E. coli and P. aeruginosa, which are in the biofilm, it is necessary that «San active» acted in a concentration not lower than 0,5% and less than 30 minutes. It asw established that the test culture of E. coli and S. aureus are not adapted to solutions of means «San active». A definition of corrosive action of detergent–disinfectant «San active» was designed for sanitary processing of technological equipment at meat processing industry. The degree of corrosion activity was determined by examining the corrosive action of solutions on medium plate, stainless steel, aluminum and galvanized iron, ie metal of construction for process equipment in meat production. It was established that working solutions of 1,0 – 2,0% detergent–disinfectant «San active» are practically not corrosive on stainless steel, because the corrosion was at times less than 200 –66 and 120 – 37 corrosion rate in times less than the acceptable norm. The rate of corrosion of galvanized iron under the influence of 1,0 – 2,0% solution of means «San active» ranged from 0,27 to 0,49 mg/m2 h, that in 2,0 – 5,4 times is higher compared to the effect on stainless steel and 44 – 24 times lower than normal. The rate of corrosion of similar detergent–disinfectants Hlorantoin, Virosan and Bioshaum to galvanized iron ranged from 0,44 to 0,49 mg/m2 h. This indicates the fact that the research variant of means «San active» for the magnitude and speed of corrosion on galvanized iron is not inferior to existing in Ukraine means. The corrosion value of aluminum for the actions of 1,0 – 2,0% solution of means «San active» was 2,2–3,7 times smaller compared to the norm, which refers to the aluminum and corrosion rate was to 1,0% solution of 2,86 mg/m2 h and 2,0% – 4,75 mg/m2 h that in 4,1 – 2,5 times less the standard value.
ВПЛИВ ПРОФІЛАКТИЧНОГО ПРОТИПНЕВМОКОКОВОГО ЗАСОБУ «ПНЕВМО-ПРО» НА МОРФОЛОГІЧНІ ЗМІНИ ОРГАНІВ ІМУННОЇ СИСТЕМИ КРОЛІВ
Authors: Storchak Y.G. , Kisera Y.V. 
Published: October 2016

There are results of the histological research of the immune system organs of rabbits after injection with a prophylactic drug «Pneumo-Pro», which includes two components: Streptococcus pneumoniae and 4% ethanol-water extract of propolis. Tentative reseaches on the development of preventive drug «Pneumo-Pro» showed enhancement of post-vaccination immunity in the application on the basis of propolis preparations. Histological reseaches of the diverticulum of the ileum and appendix found a significant amount of lymphoblasts, frequently visualized mitosis of lymphocytes in the center of submucosal lymphoid nodules is. Large, medium and small lymphocytes, macrophages are located in the dome of submucosal lymphoid nodules. The crown of the lymphoid nodules contains mainly lymphocytes of different maturity. Necrotic cells are rare. There is increase the amount of volume crowded lymphoid nodules in the lamina propria and submucosa in compare with the control group. Most of lymphoid nodules are located under the crypts, only a few of them are in contact with the intestinal lumen. Histological reseaches of the appendix of the experimental group of animals injected with «Pneumo-Pro» found B-lymphocytes, macrophages and interdigitive cells are located in the periphery of the lymphoblasts. Quite often mitosis of lymphoid cells are visualized. Some lymphocytes are exposed with necrotic changes, resulting in small intervals are present between lymphoid elements. The peripheral part of the lymphoid nodules and interfollicular zone contain a significant number of T-lymphocytes. Crypts are placed above the top of many follicles. In the lamina propria and submucosa of the appendix there are numerous, voluminous accumulations of lymphoid tissue, which form the clusters of submucosal lymphoid nodules. The reseaches indicate an increase in the functional activity of nonspecific factors of resistance and immunobiological reactivity of organism of animals after injection with a prophylactic drug «Pneumo-Pro».
КІЛЬКІСНИЙ СКЛАД МІКРОБІОТИ КИШЕЧНИКА ПОРОСЯТ У РІЗНІ ПЕРІОДИ СТРЕСУ-ВІДЛУЧЕННЯ НА ТЛІ ВИКОРИСТАННЯ ДОБАВОК «В-ГЛЮКАН» ТА «БІОВІР»
Authors: Stojanovskyj V.G. , Matsjuk O.I. , Кolotnytskyy V.A. , Kolomijets I.A. 
Published: October 2016

The article presents data of the results of the study the number of the main representatives of the intestine microflora (lakto- and bifidobacteria, E. coli, bacillus) of pigs during the weaning at 28 days of life (by the action of stress) and in different periods of development stage resistance (20 and 60 days after excommunication) for inclusion in the diet of probiotic supplements «B-glucan» and «Biovir». It is established a quantitative description of the main representatives of the microflora of small and large intestines of pigs K group at 28 days of life, a sign of the prevailing microbiota clinically healthy animals and points to the sufficient level of adaptation reactions of the organism. Under the action of stress at weaning stage resistance (20 and 60 days after weaning) adaptation of the main representatives gut of piglets K associated with lower number of bifidobacteria and lactobacilli and increasing number of E. coli average of two orders in the cavity in the wall and the thin layer of the intestines (p < 0,05). Feeding animal research groups additives «B-glucan» and «Biovir» of 5- to 45-day age contributes to the number of bifidobacteria and lactobacilli and positive dynamics of the number of E. coli in different parts of the intestine of pigs at 28 days of life and 20 and 60 days after weaning, indicating a prolonged effect of additives in the body for action piglets stress. The best effect for activation of mechanisms of adaptogenesis of piglets intestinal microbiota is showed additive «Biovir» for the actions of stress.
ПАТОМОРФОЛОГІЧНІ ОСОБЛИВОСТІ ЛЕЙДИГОМИ СОБАК
Authors: Shestiaieva N.I. , Osadchuk Y.V. 
Published: October 2016

The research for the study of morphological, clinical and macroscopic characteristics of the different histological types of canine testicular tumors. Material was obtained during a routine surgery for the removal of testicular tumors in dogs in clinics of veterinary medicine in Kiev. Tumors were divided into sex-cord stromal tumors and germ cell tumors. Among the sex-cord stromal tumors recorded leydig (interstitial) and sertoli cell tumors. Macroscopical and histologic characteristics of canine interstitial (Leydig) cell tumor are described. It is 12.5% of all testicular tumors. The average age of dogs is 9 years. Largest share is breeds dog such as foxterriers and toyterriers. Interstitial (Leydig) cell tumor has a slower growth compared to other tumors the testicle, no metastases. Half the animals had no clinical signs. Mostinterstitial (Leydig) cell tumor go together with the pathology of adnexal structures. This is because Leydig cell tumor lead to increased levels of androgens. This fact may cause pathology of adnexal structures. Neoplasms are recorded both on the right and the left testis. Leydig cell tumor was not related to the cryptorchid testicles. Most tumors regular oval, brown or gray color, different texture and size with no signs of necrosis or ulceration. The average volume of tumors ranged from 2 to 20 cm2. Microscopically, the tumor is composed of cells that resemble Leydig cells and that are arranged into islands or tubular structures. Find polygonal eosinophilic cells with granular or vacuolated, which contains lipids. Nuclei were round mesh of chromatin and distinct nucleolus. Sometimes noted the large cells with 1, 2 or more nucleoli. In some cases, found elongated fusiform cells with small nuclei and granular eosinophilic cytoplasm. Tumor stroma characterized by calcification. Note mitotically active cells. Cysts lined by tumor cells are present in some tumors. Distinction between benign and malignant forms on purely histological criteria, in the absence of metastasis, often is difficult. Studies on the features of histological structure of testicular tumors will optimize the histological diagnosis of neoplasms.
ВПЛИВ КОЕФІЦІЄНТА ПОВЕРХНЕВОГО НАТЯГУ РІДКОГО ТЕПЛОНОСІЯ НА СЕРЕДНЮ ТОВЩИНУ ПРИГРАНИЧНОГО ЛАМІНАРНОГО ШАРУ В РЕКУПЕРАТИВНИХ ТЕПЛООБМІННИКАХ
Authors: Bilonoha Y.L. , Maksysko O.R. , Bilonoha D.M. , Prykhodska S.V. 
Published: October 2016

В процесах теплообміну визначальними є гідромеханічні характеристики потоків теплоносіїв, а саме їхні режими руху. В рекуперативних теплообмінниках загальний тепловий опір системи на 95% концентрується в пристінній області потоків теплоносіїв – в ламінарному при поверхневому шарі (ЛПШ). В існуючих рівняннях для розрахунку середніх товщин ЛПШ не враховуються поверхневі характеристики рідкофазних теплоносіїв (коефіцієнта поверхневого натягу та гідрофільності поверхні змочування). Зроблено силовий аналіз елементарного об’єму рідини в ЛПШ і встановлено пріоритетну дію сил поверхневого натягу та сил тиску. Виведена формула для розрахунку середньої товщини ЛПШ з врахуванням цих сил та коефіцієнта турбулізації КТ та співвідношення Дарсі–Вейсбаха. Одержана формула містить коефіцієнти поверхневого натягу, динамічної в’язкості та густину рідкого теплоносія, гідрофільність поверхні змочування внутрішньої рекуперативної стінки, довжину та діаметр труб, числа Рейнольдса та Дарсі, коефіцієнт турбулізації потоку.
Запропоновано зменшувати середню товщину ЛПШ шляхом додавання до потоків теплоносіїв оптимальних концентрацій поверхнево–активних речовин (ПАР). Показано, що за додавання оптимальних концентрацій аніонактивних, неіоногенних та катіонактивних ПАР середня товщина ЛПШ зменшується в межах до 30%.
МАТЕМАТИЧНЕ МОДЕЛЮВАННЯ ПРОЦЕСІВ В ЕЛЕКТРОПРИВОДІ ВОДЯНОГО НАСОСА З АКУМУЛЯТОРНИМИ БАТАРЕЯМИ
Authors: Bodnar G.J. , Shapovalov O.V. , Fedyshyn J.I. , Hembara T.V. 
Published: October 2016

Розглядається схема електроживлення електроприводу водяного насоса підвищувача тиску води системи внутрішнього протипожежного водопроводу від резервного джерела з акумуляторними батареями і автономними інверторами напруги, її математична модель та результати моделювання електромагнітних і електромеханічних процесів в двигуні під час пуску і роботи насоса у випадку відсутності основного електроживлення від мережі, що забезпечує використання внутрішнього протипожежного водопроводу при надзвичайних ситуаціях протягом розрахункового часу. Така резервна система може використовуватись також для підтримки неперервності технологічних процесів.
Загальна математична модель електроприводу формувалась з математичних моделей окремих елементів схеми, які представлені багатополюсниками, а процеси в них описуються замкненою системою рівнянь, – диференційних, алгебраїчних та логічних. Розрахункову схему моделі електроприводу сформовано шляхом з’єднання між собою зовнішніх віток окремих елементів-багатополюсників, а саме: джерела живлення з акумуляторною батареєю, інверторів напруги(катодні та анодні вентильні групи), трансформаторів та асинхронного двигуна. Спосіб з’єднання між собою зовнішніх віток багатополюсників математично описується матрицями з’єднань, які складаються для кожного елемента за принципом: кількість рядків матриці рівна кількості незалежних вузлів схеми, а кількість стовпців рівна кількості зовнішніх віток елемента. Обчислення реалізовано мовою FORTRAN. Загальні підпрограми призначені для виконання математичних операцій над матрицями; чисельного інтегрування систем диференційних рівнянь методом Рунге-Кутта 2-го порядку; розв’язування систем алгебраїчних рівнянь методом Гауса; визначення моментів природного закривання вентилів. Отримано результати моделювання при прямому пуску асинхронного двигуна від мережі, встановлено струм статора; кутову швидкість обертання ротора та електромагнітний момент і момент навантаження. Результати обчислень підтверджені даними експериментальних досліджень, практично співпадають криві струму і напруги живлення асинхронного двигуна від мережі і автономного джерела з акумуляторною батареєю при пуску і роботі насоса, форма вихідної напруги джерела і тиску насоса, впродовж тривалої роботи електроприводу насоса.