bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insecticide resistance in Australian populations of the serpentine leaf miner Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae)
Authors: Nguyen Duong T. , Chen Yizhou , Herron Grant A. 
Published: October 2024

AbstractSerpentine leaf miner, Liriomyza huidobrensis, invaded New South Wales and Queensland late 2020. Overseas, L. huidobrensis has a history of developing insecticide resistance and, since its Australian discovery, has caused significant control issues in Australian horticultural industries. Here, we developed bioassay methods to test the response of L. huidobrensis larvae to spirotetramat and L. huidobrensis adults to dimethoate, imidacloprid and chlorantraniliprole. Against two New South Wales and one Queensland collected strains, we found a 33‐fold elevated response against dimethoate and 200‐fold more against imidacloprid than the field‐recommended field rates. Furthermore, we found that a maximum rate of 64‐fold the field‐recommended rate of chlorantraniliprole controlled only 94% of one tested population. Similarly, the maximum rate of spirotetramat tested (64‐fold the field‐recommended rate) only achieved 84%–94% mortality for the same populations. Prior to our study, the efficacy of chlorantraniliprole and spirotetramat against Liriomyza was poorly studied, with only a few reports with chlorantraniliprole against Liriomyza trifolii. Consequently, none of the tested insecticides in this study will likely control Australian invasive L. huidobrensis at the field‐recommended rates. Our results presented here highlight the need to identify and study alternative chemical and biological products and natural enemies for the control of L. huidobrensis in Australian horticulture.
Imatinib decreases germ cell survival and germline stem cell proliferation in rodent testis ex vivo and in vitro
Authors: Eggert Anna , Laasanen Sini , Nurmio Mirja , Wahlgren Aida , Jahnukainen Kirsi , Eerola Kim , Nieminen Miisael , Olotu Opeyemi , Kotaja Noora , Mäkelä Juho‐Antti , Toppari Jorma 
Published: October 2024

AbstractBackgroundImatinib and dasatinib are tyrosine kinase inhibitors (TKIs) increasingly used to treat several diseases in both children and adults at fertile age. We have previously shown that imatinib has adverse effects on developing testis, and imatinib‐treated male patients have been reported to have reduced sperm counts. However, the cellular level effects of imatinib and dasatinib on adult male germ cells and germline stem cells (mGSCs) have not been thoroughly investigated.ObjectivesTo analyze whether imatinib or dasatinib exposure ex vivo and in vitro is harmful to adult male rodent germ cells and mGSCs.Materials and methodsSeminiferous tubule segments of adult male mouse or rat were cultured in the presence or the absence of imatinib or dasatinib. Stage‐specific effects were monitored by 3H‐thymidine incorporation assay (DNA synthesis), immunohistochemistry (cleaved Caspase‐3; apoptosis), immunofluorescence (KI67, GFRα1, STRA8, c‐KIT, LIN28A; proliferation and spermatogonial differentiation) and flow cytometry (Hoechst). Mouse mGSCs were exposed to imatinib and dasatinib to study proliferation, apoptosis, and differentiation.ResultsImatinib decreased stage‐specific DNA synthesis, and induced apoptosis in cultured rat seminiferous tubule segments. Imatinib also had an adverse effect on mGSC proliferation both in vitro and ex vivo, but did not induce cell death in cultured mGSCs. Imatinib did not impinge on induction of spermatogonial differentiation but suppressed c‐KIT expression in nascent differentiating spermatogonia, providing a plausible mechanism for its pro‐apoptotic function in spermatogenic cells. Clinically relevant doses of dasatinib did not induce apoptosis in seminiferous tubules but decreased mGSC colony growth in vitro.ConclusionsImatinib exposure ex vivo and in vitro impinges on male rodent germ cell proliferation and survival. The plausible mechanism in spermatogenic cells is the inhibition of SCF/c‐KIT signaling, and reduced expression of c‐KIT. Dasatinib did not show significant adverse effects with clinical doses ex vivo but inhibited mGSC colony growth in vitro.
Interspecific interactions among major carnivores in Panna Tiger Reserve: A multispecies occupancy approach
Authors: Dutta Supratim , Maheswaran Gopinathan , Krishnamurthy Ramesh 
Published: October 2024

AbstractLarge carnivores play a crucial role in trophic cascades, affecting the population dynamics of both co‐predators and prey within an ecosystem. Understanding the significance of these carnivores in trophic interactions is essential for developing effective conservation and management strategies. We examined the effects of occupancy dynamics and patterns of species interactions and coexistence within the carnivore guild in the Panna Tiger Reserve in India. We collected camera trap data (two seasons, 2019) in a presence–absence framework and applied multispecies occupancy models to assess the occupancy, co‐occurrence, and interactions among species. We also examined activity overlap to understand the temporal segregation in the carnivore guild. The mean marginal occupancy was highest for leopards in winter (Ψwinter 0.92 ± 0.02, Ψsummer 0.63 ± 0.05) and hyenas in summer (Ψsummer 0.93 ± 0.03, Ψwinter 0.78 ± 0.03) and was lowest for tigers in both seasons (Ψwinter 0.62 ± 0.05, Ψsummer 0.15 ± 0.05). Co‐occurrence probability among carnivores was higher in winter than in summer, and conditional occupancy was consistently higher when other species were present. Different environmental factors influenced marginal occupancy and co‐occurrence patterns across seasons. Strong temporal overlaps were recorded between tiger–leopard (0.87–0.91) and tiger–hyena (0.78–0.79). We detected a significant spatial segregation between tigers and leopards, as they prefer different habitat types in different seasons, along with high temporal overlap. Resource availability strongly governs the association of carnivores with their habitat selection. Hyenas demonstrated higher dependency on tigers than on leopards for resources. These findings indicate that coexistence with apex‐predator species is feasible through strategic adaptation to fulfill resource requisition.
Mid‐term outcomes of valve replacement surgery with or without coronary artery bypass grafting in patients with overweight and obesity: A cohort study
Authors: Rajablou Nadia , Tashak Golroudbari Hasti , Ahmadi Tafti Seyed Hossein , Bagheri Jamshid , Zoroufian Arezou , Sahebjam Mohammad , Salehbeigi Shahrzad , Lesani Zohreh , Omidi Negar 
Published: October 2024

SummaryCurrent data evaluating outcomes of valve replacement surgery in patients with overweight/obesity is contradictory. There is a scarce study comparing outcomes of valve surgery considering the type of valve involved in the procedure. We followed outcomes in patients with overweight and obesity after valve replacement surgery with or without coronary artery bypass graft (CABG) and also patients with aortic valve replacement (AVR) and mitral valve replacement (MVR), separately to compare their mid‐term prognosis in each group. Consecutive patients who had undergone cardiac valve surgery with or without CABG in Tehran Heart Center were enrolled. We enrolled 3158 patients. Median survival was 125.71 ± 82.20 weeks in patients with overweight/obesity. We found a significantly higher LVDd, LVDs and RVDd in patients with CABG (51.85 ± 7.31, 36.80 ± 8.81 and 30.04 ± 3.58, respectively) compared to the valve group (50.10 ± 6.35 mm, 35.08 ± 7.29 mm and 29.76 ± 4.07 mm, respectively). All‐cause mortality is significantly higher in patients with AVR (5.7%) than those with MVR (3.3%). Patients with CABG are at higher risk of myocardial infarction (MI) compared to the ones without CABG (0.8% vs. 0.1%). No significant interaction was observed between the type of valve surgery (AVR/MVR) or valve surgery combinations (valve/valve + CABG) (p‐values = .81 vs. .97, respectively). Post‐operative outcomes in patients with overweight/obesity depend on several factors such as type of valve involved and presence of CABG. Risk management can lower the rate of mortality and morbidity in these patients.
Delineating the neural substrates of autobiographical memory impairment in Huntington's disease
Authors: Horne Kristina , Carmichael Anna , Mercieca Emily‐Clare , Glikmann‐Johnston Yifat , Stout Julie C. , Irish Muireann 
Published: October 2024

AbstractEmerging evidence suggests that autobiographical memory (ABM) is altered in Huntington's disease (HD). While these impairments are typically attributed to frontostriatal dysfunction, the neural substrates of ABM impairment in HD remain unexplored. To this end, we assessed ABM in 30 participants with genetically confirmed HD (18 premanifest, 12 manifest) and 24 age‐matched healthy controls. Participants completed the Autobiographical Interview to assess free and probed ABM recall and underwent structural brain imaging. Whole‐brain voxel‐based morphometry (VBM) was used to explore voxel‐wise associations between ABM performance and grey matter intensity (False Discovery Rate corrected at q = 0.05). Relative to controls, HD participants displayed significantly less detailed ABM retrieval across free and probed recall conditions, irrespective of disease stage. Recall performance did not differ significantly between manifest and premanifest HD groups. VBM analyses indicated that poorer ABM performance was associated with atrophy of a distributed cortico‐subcortical network. Key regions implicated irrespective of ABM condition included the bilateral occipital cortex, left precuneus, right parahippocampal gyrus and right caudate nucleus. In addition, probed ABM recall was associated with the superior and inferior frontal gyri, frontal pole, right hippocampus, nucleus accumbens, paracingulate gyrus and cerebellum. Overall, our findings indicate that ABM impairments in HD reflect the progressive degeneration of a distributed cortico‐subcortical brain network comprising medial temporal, frontal, striatal and posterior parietal cortices. Our findings advance our understanding of the neurocognitive profile of HD, providing an important foundation for future interventions to support memory function in this population.
Basic or applied research in university–industry collaborations? An analysis of research orientations in knowledge creation partnerships
Authors: Cabanes Benjamin , Plantec Quentin , Le Masson Pascal , Weil Benoît 
Published: October 2024

AbstractIn a knowledge‐based economy, university–industry collaborations play a pivotal role in driving innovation and economic growth. This study investigates the research orientations of these collaborations, focusing on the balance between basic and applied research. Using data from 631 collaborative projects funded by the French Cifre PhD programme, we find that most projects emphasize basic research (61%), contradicting the common expectation that industry‐led partnerships primarily focus on applied research. Importantly, our analysis reveals that this preference for basic research is not contingent on traditional contextual factors such as scientific discipline, company size or university type. These findings challenge established assumptions in the literature and suggest that even in industry‐sponsored research, fundamental inquiry is critical in supporting long‐term innovation strategies. This paper contributes to the understanding of research orientations in university–industry collaborations and offers practical suggestions for enhancing these partnerships and informing research policy. Additionally, we outline a research agenda for further exploration of the factors influencing research orientations, the impact of early‐stage industry engagement and the role of policies in shaping collaboration outcomes.
Implications of the non‐neuronal cholinergic system for therapeutic interventions of inflammatory skin diseases
Authors: Qu Hui‐Qi , Kao Charlly , Hakonarson Hakon 
Published: October 2024

AbstractThe pivotal roles of acetylcholine (ACh) in physiological processes encompass both the nervous and non‐neuronal cholinergic systems (NNCS). This review delineates the synthesis, release, receptor interactions, and degradation of ACh within the nervous system, and explores the NNCS in depth within skin cells including keratinocytes, endothelial cells, fibroblasts, macrophages, and other immune cells. We highlight the NNCS's essential functions in maintaining epidermal barrier integrity, promoting wound healing, regulating microcirculation, and modulating inflammatory responses. The potential of the NNCS as a therapeutic target for localized ACh regulation in the skin is discussed, though the translation of these findings into clinical practice remains uncertain due to the complexity of cholinergic signalling and the lack of comprehensive human studies. The review progresses to therapeutic modulation strategies of the NNCS, including AChE inhibitors, nicotinic and muscarinic receptor agonists and antagonists, choline uptake enhancers, and botulinum toxin, highlighting their relevance in dermatology. We highlight the impact of the NNCS on prevalent skin diseases such as psoriasis, atopic dermatitis, rosacea, acne, bullous diseases, hyperhidrosis and hypohidrosis, illustrating its significance in disease pathogenesis and therapy. This comprehensive overview aims to enhance understanding of the NNCS's role in skin health and disease, offering a foundation for future research and therapeutic innovation.
TROP2 Expression and Therapeutic Implications in Cutaneous Squamous Cell Carcinoma: Insights From Immunohistochemical and Functional Analysis
Authors: Tanegashima Keiko , Tanaka Yuka , Ito Takamichi , Oda Yoshinao , Nakahara Takeshi 
Published: October 2024

ABSTRACTCutaneous squamous cell carcinoma (cSCC) is a common form of skin cancer, but treatments for advanced cases have limited efficacy. Trophoblast cell‐surface antigen 2 (TROP2) is a cell‐surface protein that is widely expressed in various tumours, where it exerts significant influence over critical processes such as tumour cell growth, apoptosis, migration, invasion and metastasis. Sacituzumab govitecan, an antibody‐drug conjugate (ADC) targeting TROP2, is emerging as a promising strategy for anticancer therapy. In this study, we investigated TROP2 expression in cSCC tissues from 51 patients and evaluated its function in the A431 human SCC cell line. Immunohistochemical analysis revealed TROP2 expression on the plasma membrane of cSCC tissues and A431 cells. A431 cells showed sensitivity to sacituzumab govitecan with a significant concentration‐dependent decrease in viable cell number. In addition, Knockdown of TROP2 resulted in decreased expression of cyclin D1 and BCL‐2, along with reduced cell viability. Knockdown of TROP2 also resulted in decreased expression of vimentin, along with reduced migratory capacity. These findings suggest that TROP2 plays a crucial role in cSCC cell proliferation and migration, and highlight the potential of sacituzumab govitecan as a promising therapeutic option for cSCC.
Treatment of a patient with inoperable tumoral calcinosis associated with end stage kidney disease: A case report
Authors: Couser Sarah K. , Claes Donna J. , Huesman Sydney , Hooper David K. 
Published: October 2024

AbstractWe describe a case of severe symptomatic tumoral calcinosis in a young man with end stage kidney disease secondary to antineutrophil cytoplasmic antibodies‐associated vasculitis with longstanding hyperphosphatemia and secondary hyperparathyroidism while on several years of peritoneal dialysis. The use of intravenous sodium thiosulfate, optimization of clearance with five times weekly hemodialysis, and intradialytic nutrition were used to treat his inoperable tumoral calcinosis. Over 3 months, he had a remarkable reduction in the size of his calcified masses and associated improvement in pain. He subsequently received a living donor kidney transplant.
The Experiences and Perceptions of Telehealth in Patients Living With Advanced Chronic Obstructive Pulmonary Disease: A Qualitative Evidence Synthesis
Authors: O'Connor Úna , Crilly Geraldine 
Published: October 2024

ABSTRACTAimTo systematically identify, appraise and synthesise qualitative research evidence which examined the impact of telehealth on the experiences and perceptions of patients living with advanced chronic obstructive pulmonary disease, to inform the development of patient‐centred telehealth.DesignQualitative evidence synthesis.Database SearchesCINAHL, Cochrane, Embase, PUBMED, MEDLINE, EThOS, Web of Science, PsycINFO, Lenus, DART, RIAN and ProQuest were searched for primary qualitative studies undertaken between 2008 and 2023.MethodsA thematic synthesis of studies was undertaken to identify descriptive themes relating to patient views. Methodological quality was assessed using the Critical Appraisal Skills Programme framework, and confidence in review findings was assessed using the GRADE‐CERQual approach.FindingsNine studies met the inclusion criteria and were included in the final synthesis. Four analytical themes were generated (1) telehealth as a facilitator of independence, (2) the influence of patient and healthcare provider relationship on successful engagement with telehealth, (3) usability of telehealth to patients living with advanced chronic obstructive pulmonary disease and (4) trusting virtual health services and facilitating confidence in the patient/service user. Five descriptive themes emerged: (i) individualised telehealth chronic obstructive pulmonary disease care (ownership and control), (ii) managing chronic obstructive pulmonary disease exacerbations, (iii) being heard and feeling understood, (iv) telehealth as an education aid and (v) aging and virtual technology.ConclusionUnderstanding the experiences of patients with chronic obstructive pulmonary disease and their engagement with telehealth is a necessary determinant of how best to utilise telehealth in this population and may serve to inform policymakers to further develop and implement telehealth into practice. Future research on patients and healthcare professionals' views on telehealth use in the palliative stage of this illness may also be valuable.ImpactFindings add value by providing healthcare providers with additional evidence to improve understanding of both telehealth complexity and human experiences and perceptions. It is anticipated that a deeper understanding of chronic obstructive pulmonary disease patients' experiences and perceptions will inform the development of strategies to maximise and enhance the application of patient‐centred telehealth within the context of coping and living with a debilitating condition.Patient or Public ContributionNo patient or public contribution was utilised in this study.
The role of different types of knowledge and expertise in explaining recognition justice in flood defence and flood risk prevention
Authors: Paauw Mandy , Crabbé Ann , Guevara Viquez Sofia , Priest Sally 
Published: October 2024

AbstractFlood risks worldwide are rising and it is increasingly recognised that the impacts of floods are not neutral. Socioeconomic and demographic characteristics determine people's capacity to deal with flood events. These differences in social vulnerability to floods need to be considered in flood risk management (FRM) to prevent the most vulnerable groups from being disproportionately impacted. However, due to a diversification of FRM strategies and the involvement of various policy domains, the experts working on FRM are no longer a homogeneous group. Where FRM was previously dominated by engineers, now various experts are involved that have different disciplinary backgrounds, knowledge bases and approaches to FRM. As a result, they also differ in their recognition of social vulnerability to floods. In this paper, we explore the different types of knowledge and expertise in FRM in three countries (England, Flanders and France), focussing on the strategies of flood defence and flood risk prevention. We characterise the epistemic communities supporting the domains and study to what extent experts differ in their recognition of social vulnerability to floods. We also dive into the mechanisms employed to stimulate integration between experts and consider the extent to which this integration can strengthen recognition justice.
Mental workload as a predictor of burnout in intensive care nurses
Authors: Gündüz Emine Selda , Öztürk Nilgün Kavrut 
Published: October 2024

AbstractBackgroundNurses face a high level of mental workload while performing their duties. High levels of mental workload can negatively impact nurses.AimThe aim of this research was to determine the relationship between mental workload and burnout levels of intensive care nurses.Study designDescriptive‐correlational design has been adopted in this research. The sample consisted of 156 nurses working in the intensive care units of a hospital in the south of Turkey between September and December 2023. Research data were collected with the ‘Participant Information Form’, which includes socio‐demographic characteristics, ‘Mental Workload Scale’ and ‘Maslach Burnout Scale’.ResultsAlmost all (95.5%) of the nurses participating in the study reported that their mental workload was high. It has been determined that there is a strong positive relationship between mental workload and burnout. According to the regression model obtained from the research, being a woman (β = .165, p = .008) and emotional workload (β = −695, p < .001) are determinants of emotional exhaustion. These variables explain emotional exhaustion at a rate of 50.1% (adjusted R2 = .501). Emotional workload (β = .399, p = .003) is the most important determinant of personal accomplishment. This variable explains 19.0% (adjusted R2 = .190) of personal accomplishment. The most important determinant of depersonalization is emotional workload (β = .324, p < .001). It was determined that this variable explained 24.4% (adjusted R2 = .244) of depersonalization.ConclusionsThis research revealed that there is a strong positive relationship between mental workload and burnout and that increasing mental workload can increase burnout.Relevance to Clinical PracticeNurses' risk of emotional exhaustion increases as mental workload increases. Reducing cognitive workload, temporary workload and emotional workload increases the personal accomplishment experienced by nurses. In addition, as temporary workload, emotional workload and performance‐related workload increase, the depersonalization experienced by nurses also increases.