bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence, species and antimicrobial resistance of naturally occurring Campylobacter isolates from quail carcasses sampled in a commercial processing facility
Authors: Cox N. A. , Cosby D. E. , Thippareddi H. , Ritz C. W. , Berrang M. E. , Jackson J. S. , Mize S. C. , Kumar S. , Howard A. K. , Rincon A. M. , Ukidwe M. S. , Landrum M. A. , Frye J. G. , Plumblee Lawrence J. R. , Hiott L. M. , Jackson C. R. , Hinton A. , Cook K. L. 
Published: January 2018

AbstractThere is limited information about Campylobacter in commercially processed quail (Coturnix coturnix). The objective of this study was to determine the occurrence of Campylobacter on carcasses in a commercial quail processing plant. Carcasses were collected prior to chilling and/or the application of any antimicrobials, transported to the laboratory, and individually bagged. Following the standard protocol for recovering Campylobacter, three typical colonies were selected, confirmed as Campylobacter and tested for antimicrobial susceptibility. Nine replicates were conducted. Of the 85 carcasses obtained (n = 5 for first visit, n = 10 for 8 subsequent visits), 28 (32.9%) carcasses were found to be positive for Campylobacter. On four visits, no Campylobacter was found; on two visits, all samples were positive; and 1/5, 1/10, and 6/10 samples were positive for the remaining visits. Of the 28 isolates recovered, 18 (64%) were C. jejuni and 10 (36%) were C. coli. Tetracycline resistance was detected in all 28 isolates. Future work in the breeder flocks, hatchery, and grow‐out houses is planned to further understand Campylobacter ecology in quail production and processing which could also be useful to the broiler industry.Practical applicationsVery little published information exists for the presence of Campylobacter spp. on processed quail. Most of the studies involve cloacal swabs and ceca from samples obtained from hunters. This study involves sampling processed quail carcasses from a large commercial integrated company and also determined the species and antibiotic resistance profile of the Campylobacter isolates. There is considerable debate concerning the ecology of Campylobacter on poultry and perhaps our data can be used by the broiler industry to develop a better understanding of the ecology and to assist in the application of effective intervention strategies. Presently, there is slightly under 100,000 million commercially processed and domestically grown quails in the United States each year, most of which is marketed in Europe as a frozen product. So the presence of an important foodborne pathogen on this food is significant information.
EXIOBASE 3: Developing a Time Series of Detailed Environmentally Extended Multi‐Regional Input‐Output Tables
Authors: Stadler Konstantin , Wood Richard , Bulavskaya Tatyana , Södersten Carl‐Johan , Simas Moana , Schmidt Sarah , Usubiaga Arkaitz , Acosta‐Fernández José , Kuenen Jeroen , Bruckner Martin , Giljum Stefan , Lutter Stephan , Merciai Stefano , Schmidt Jannick H. , Theurl Michaela C. , Plutzar Christoph , Kastner Thomas , Eisenmenger Nina , Erb Karl‐Heinz , de Koning Arjan , Tukker Arnold 
Published: January 2018

SummaryEnvironmentally extended multiregional input‐output (EE MRIO) tables have emerged as a key framework to provide a comprehensive description of the global economy and analyze its effects on the environment. Of the available EE MRIO databases, EXIOBASE stands out as a database compatible with the System of Environmental‐Economic Accounting (SEEA) with a high sectorial detail matched with multiple social and environmental satellite accounts. In this paper, we present the latest developments realized with EXIOBASE 3—a time series of EE MRIO tables ranging from 1995 to 2011 for 44 countries (28 EU member plus 16 major economies) and five rest of the world regions. EXIOBASE 3 builds upon the previous versions of EXIOBASE by using rectangular supply‐use tables (SUTs) in a 163 industry by 200 products classification as the main building blocks. In order to capture structural changes, economic developments, as reported by national statistical agencies, were imposed on the available, disaggregated SUTs from EXIOBASE 2. These initial estimates were further refined by incorporating detailed data on energy, agricultural production, resource extraction, and bilateral trade. EXIOBASE 3 inherits the high level of environmental stressor detail from its precursor, with further improvement in the level of detail for resource extraction. To account for the expansion of the European Union (EU), EXIOBASE 3 was developed with the full EU28 country set (including the new member state Croatia). EXIOBASE 3 provides a unique tool for analyzing the dynamics of environmental pressures of economic activities over time.
Geospatial Characterization of Material Stock in the Residential Sector of a Latin‐American City
Authors: Mesta Carlos , Kahhat Ramzy , Santa‐Cruz Sandra 
Published: January 2018

SummaryBuilding stock constitutes a huge repository of construction materials in a city and a potential source for replacing primary resources in the future. This article describes the application of a methodological approach for analyzing the material stock (MS) in buildings and its spatial distribution at a city‐wide scale. A young Latin‐American city, the city of Chiclayo in Peru, was analyzed by combining geographical information systems (GIS) data, census information, and data collected from different sources. Application of the methodology yielded specific indicators for the physical size of buildings (i.e., gross floor area and number of stories) and their material composition. The overall MS in buildings, in 2007, was estimated at 24.4 million tonnes (Mt), or 47 tonnes per capita. This mass is primarily composed of mineral materials (97.7%), mainly concrete (14.1 Mt), while organic materials (e.g., 0.15 Mt of wood) and metals (e.g., 0.40 Mt of steel) constitute the remaining share (2.3%). Moreover, historical census data and projections were used to evaluate the changes in the MS from 1981 to 2017; showing a 360% increase of the MS in the last 36 years. This study provides essential supporting information for urban planners, helping to provide a better understanding of the availability of resources in the city and its future potential supply for recycling as well as to develop strategies for the management of construction and demolition waste.
A guide to analysis and reconstruction of serial block face scanning electron microscopy data
Authors: COCKS E. , TAGGART M. , RIND F.C. , WHITE K. 
Published: January 2018

SummarySerial block face scanning electron microscopy (SBF‐SEM) is a relatively new technique that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural data. There is a wealth of information that can be obtained from the resulting image stacks but this presents a new challenge for researchers – how to computationally analyse and make best use of the large datasets produced. One approach is to reconstruct structures and features of interest in 3D. However, the software programmes can appear overwhelming, time‐consuming and not intuitive for those new to image analysis. There are a limited number of published articles that provide sufficient detail on how to do this type of reconstruction. Therefore, the aim of this paper is to provide a detailed step‐by‐step protocol, accompanied by tutorial videos, for several types of analysis programmes that can be used on raw SBF‐SEM data, although there are more options available than can be covered here. To showcase the programmes, datasets of skeletal muscle from foetal and adult guinea pigs are initially used with procedures subsequently applied to guinea pig cardiac tissue and locust brain. The tissue is processed using the heavy metal protocol developed specifically for SBF‐SEM. Trimmed resin blocks are placed into a Zeiss Sigma SEM incorporating the Gatan 3View and the resulting image stacks are analysed in three different programmes, Fiji, Amira and MIB, using a range of tools available for segmentation. The results from the image analysis comparison show that the analysis tools are often more suited to a particular type of structure. For example, larger structures, such as nuclei and cells, can be segmented using interpolation, which speeds up analysis; single contrast structures, such as the nucleolus, can be segmented using the contrast‐based thresholding tools. Knowing the nature of the tissue and its specific structures (complexity, contrast, if there are distinct membranes, size) will help to determine the best method for reconstruction and thus maximize informative output from valuable tissue.
The challenges and possibilities of public access defibrillation
Authors: Ringh M. , Hollenberg J. , Palsgaard‐Moeller T. , Svensson L. , Rosenqvist M. , Lippert F. K. , Wissenberg M. , Malta Hansen C. , Claesson A. , Viereck S. , Zijlstra J. A. , Koster R. W. , Herlitz J. , Blom M. T. , Kramer‐Johansen J. , Tan H. L. , Beesems S. G. , Hulleman M. , Olasveengen T. M. , Folke F. ,   for the COSTA study group (research collaboration between Copenhagen, Oslo, STockholm, and Amsterdam)
Published: January 2018

AbstractOut‐of‐hospital cardiac arrest (OHCA) is a major health problem that affects approximately four hundred and thousand patients annually in the United States alone. It is a major challenge for the emergency medical system as decreased survival rates are directly proportional to the time delay from collapse to defibrillation. Historically, defibrillation has only been performed by physicians and in‐hospital. With the development of automated external defibrillators (AEDs), rapid defibrillation by nonmedical professionals and subsequently by trained or untrained lay bystanders has become possible. Much hope has been put to the concept of Public Access Defibrillation with a massive dissemination of public available AEDs throughout most Western countries. Accordingly, current guidelines recommend that AEDs should be deployed in places with a high likelihood of OHCA. Despite these efforts, AED use is in most settings anecdotal with little effect on overall OHCA survival. The major reasons for low use of public AEDs are that most OHCAs take place outside high incidence sites of cardiac arrest and that most OHCAs take place in residential settings, currently defined as not suitable for Public Access Defibrillation. However, the use of new technology for identification and recruitment of lay bystanders and nearby AEDs to the scene of the cardiac arrest as well as new methods for strategic AED placement redefines and challenges the current concept and definitions of Public Access Defibrillation. Existing evidence of Public Access Defibrillation and knowledge gaps and future directions to improve outcomes for OHCA are discussed. In addition, a new definition of the different levels of Public Access Defibrillation is offered as well as new strategies for increasing AED use in the society.
Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders
Authors: Şimşek Kaya G. , Yapıcı Yavuz G. , Kızıltunç A. 
Published: January 2018

SummaryThe synovial membrane and fluid are significantly involved in the pathogenesis of temporomandibular joint (TMJ) disorders. This study aimed to investigate the relation between levels of chemerin in the synovial fluid (SF) of patients with TMJ disorder and their relationship. Sixty samples of SF were obtained from patients with an internal derangement (ID) or osteoarthritis (OA). Chemerin in the SF was examined by enzyme‐linked immunosorbent assay (ELISA). The results showed greater levels of chemerin in the SF of patients with OA than ID. While chemerin levels were positively correlated with pain scores, they were inversely correlated with MMO. Chemerin levels increased progressively as the disorder stage became more severe. The findings of this study suggest that chemerin in SF may play role as a predisposing factor and may represent a novel potential prognostic biochemical marker in the pathogenesis of TMJ disorders.
Gender and European Economic Policy: A Survey of the Views of European Economists on Contemporary Economic Policy
Authors: May Ann Mari , McGarvey Mary G. , Kucera David 
Published: January 2018

SummaryIn this study, we survey male and female economists located at universities in the European Union to determine if there are gender differences in views on core economic principles and a wide variety of contemporary policy issues. Controlling for country of residence, degree vintage, and location of Ph.D. earned, we find statistically significant differences in views of male and female economists in all of the topic areas examined. These results lend support to the notion that gender diversity in policy‐making circles may be an important aspect in broadening the menu of public policy choices.
Climate‐driven mitochondrial selection: A test in Australian songbirds
Authors: Lamb Annika Mae , Gan Han Ming , Greening Chris , Joseph Leo , Lee Yin Peng , Morán‐Ordóñez Alejandra , Sunnucks Paul , Pavlova Alexandra 
Published: January 2018

AbstractDiversifying selection between populations that inhabit different environments can promote lineage divergence within species and ultimately drive speciation. The mitochondrial genome (mitogenome) encodes essential proteins of the oxidative phosphorylation (OXPHOS) system and can be a strong target for climate‐driven selection (i.e., associated with inhabiting different climates). We investigated whether Pleistocene climate changes drove mitochondrial selection and evolution within Australian birds. First, using phylogeographic analyses of the mitochondrial ND2 gene for 17 songbird species, we identified mitochondrial clades (mitolineages). Second, using distance‐based redundancy analyses, we tested whether climate predicts variation in intraspecific genetic divergence beyond that explained by geographic distances and geographic position. Third, we analysed 41 complete mitogenome sequences representing each mitolineage of 17 species using codon models in a phylogenetic framework and a biochemical approach to identify signals of selection on OXPHOS protein‐coding genes and test for parallel selection in mitolineages of different species existing in similar climates. Of 17 species examined, 13 had multiple mitolineages (range: 2–6). Climate was a significant predictor of mitochondrial variation in eight species. At least two amino acid replacements in OXPHOS complex I could have evolved under positive selection in specific mitolineages of two species. Protein homology modelling showed one of these to be in the loop region of the ND6 protein channel and the other in the functionally critical helix HL region of ND5. These findings call for direct tests of the functional and evolutionary significance of mitochondrial protein candidates for climate‐associated selection.
Antimicrobial and stress responses to increased temperature and bacterial pathogen challenge in the holobiont of a reef‐building coral
Authors: van de Water Jeroen A. J. M. , Chaib De Mares Maryam , Dixon Groves B. , Raina Jean‐Baptiste , Willis Bette L. , Bourne David G. , van Oppen Madeleine J. H. 
Published: January 2018

AbstractGlobal increases in coral disease prevalence have been linked to ocean warming through changes in coral‐associated bacterial communities, pathogen virulence and immune system function. However, the interactive effects of temperature and pathogens on the coral holobiont are poorly understood. Here, we assessed three compartments of the holobiont (host, Symbiodinium and bacterial community) of the coral Montipora aequituberculata challenged with the pathogen Vibrio coralliilyticus and the commensal bacterium Oceanospirillales sp. under ambient (27°C) and elevated (29.5 and 32°C) seawater temperatures. Few visual signs of bleaching and disease development were apparent in any of the treatments, but responses were detected in the holobiont compartments. V. coralliilyticus acted synergistically and negatively impacted the photochemical efficiency of Symbiodinium at 32°C, while Oceanospirillales had no significant effect on photosynthetic efficiency. The coral, however, exhibited a minor response to the bacterial challenges, with the response towards V. coralliilyticus being significantly more pronounced, and involving the prophenoloxidase‐activating system and multiple immune system‐related genes. Elevated seawater temperatures did not induce shifts in the coral‐associated bacterial community, but caused significant gene expression modulation in both Symbiodinium and the coral host. While Symbiodinium exhibited an antiviral response and upregulated stress response genes, M. aequituberculata showed regulation of genes involved in stress and innate immune response processes, including immune and cytokine receptor signalling, the complement system, immune cell activation and phagocytosis, as well as molecular chaperones. These observations show that M. aequituberculata is capable of maintaining a stable bacterial community under elevated seawater temperatures and thereby contributes to preventing disease development.
Combining allele frequency and tree‐based approaches improves phylogeographic inference from natural history collections
Authors: Ruffley Megan , Smith Megan L. , Espíndola Anahí , Carstens Bryan C. , Sullivan Jack , Tank David C. 
Published: January 2018

AbstractModel selection approaches in phylogeography have allowed researchers to evaluate the support for competing demographic histories, which provides a mode of inference and a measure of uncertainty in understanding climatic and spatial influences on intraspecific diversity. Here, to rank all models in the comparison set and determine what proportion of the total support the top‐ranked model garners, we conduct model selection using two analytical approaches—allele frequency‐based, implemented in fastsimcoal2, and gene tree‐based, implemented in phrapl. We then expand this model selection framework by including an assessment of absolute fit of the models to the data. For this, we utilize DNA isolated from existing natural history collections that span the distribution of red alder (Alnus rubra) in the Pacific Northwest of North America to generate genomic data for the evaluation of 13 demographic scenarios. The quality of DNA recovered from herbarium specimen leaf tissue was assessed for its utility and effectiveness in demographic model selection, specifically in the two approaches mentioned. We present strong support for the use of herbarium tissue in the generation of genomic DNA, albeit with the inclusion of additional quality control checks prior to library preparation and analyses with multiple approaches that incorporate various data. Analyses with allele frequency spectra and gene trees predominantly support A. rubra having experienced an ancient vicariance event with intermittent and frequent gene flow between the disjunct populations. Additionally, the data consistently fit the most frequently selected model, corroborating the model selection techniques. Finally, these results suggest that the A. rubra disjunct populations do not represent separate species.
On the process of national indifferentiation: the case of Bulgarian ‘Czechs’
Authors: Jakoubek Marek 
Published: January 2018

AbstractAs national groups are concerned, constructivist argumentation typically follows the process of establishing national identities. Thus, it commonly studies the development of a nationally indifferent population to a population that is nationally conscious. On a general level, this paper analyses and illustrates the opposite process, i.e., the process of ‘denationalization’, or in other words, the emergence of national indifference (i.e. national indifferentiation). I study how nationally conscious groups of Czech colonists from the military frontier, who in the 1820s settled in the village of Svatá Helena in Banat gradually became a nationally indifferent group (mainly after their migration to Bulgaria where they founded the village of Voyvodovo) whose defining mark and principle of organisation became religion.
Self‐screening Effects of Monitoring: Evidence from a Quasi Experiment in the Swedish Temporary Parental Benefit Program
Authors: Häkkinen Skans Iida , Johansson Per 
Published: January 2018

AbstractMonitoring and screening have been shown to be important to reduce moral hazard in social insurances. This paper empirically investigates whether monitoring in the Swedish temporary parental benefit program affects future benefit take‐up. Identification is based on the fact that parents’ benefit applications are monitored randomly by the insurer. The estimation results show that parents who are monitored are less likely to apply again in the near future.
Home alone: a systematic review and meta‐analysis on the effects of individual housing on body weight, food intake and visceral fat mass in rodents
Authors: Schipper L. , Harvey L. , van der Beek E. M. , van Dijk G. 
Published: January 2018

SummaryRats and mice are widely used to study environmental effects on psychological and metabolic health. Study designs differ widely and are often characterized by varying (social) housing conditions. In itself, housing has a profound influence on physiology and behaviour of rodents, affecting energy balance and sustainable metabolic health. However, evidence for potential long‐term consequences of individual versus social housing on body weight and metabolic phenotype is inconsistent. We conducted a systematic literature review and meta‐analyses assessing effects of individual versus social housing of rats and mice, living under well‐accepted laboratory conditions, on measures of metabolic health, including body weight, food intake and visceral adipose tissue mass. Seventy‐one studies were included in this review; 59 were included in the meta‐analysis. Whilst housing did not affect body weight, both food intake and visceral adipose tissue mass were significantly higher in individually compared with socially housed animals. A combination of emotional stress and lack of social thermoregulation likely contributed to these effects. Increased awareness of consequences and improved specifications of housing conditions are necessary to accurately evaluate efficacy of drugs, diets or other interventions on metabolic and other health outcomes because housing conditions are rarely considered as possible moderators of reported outcomes.
Mast cells participate in chronic low‐grade inflammation within adipose tissue
Authors: Żelechowska P. , Agier J. , Kozłowska E. , Brzezińska‐Błaszczyk E. 
Published: January 2018

SummaryObesity is reckoned as one of the civilization diseases, posing a considerable global health issue. Evidence points towards a contribution of multitude immune cell populations in obesity pathomechanism and the development of chronic low‐grade inflammation in the expanded adipose tissue. Notably, adipose tissue is a reservoir of mast cells which number in individuals with obesity particularly increased. Some of them tend to degranulation what generate secretion of strong pro‐inflammatory and regulatory mediators, as well as cytokines/chemokines. Several lines of evidence suggest that mast cells are strictly associated with pro‐inflammatory status in adipose tissue by their indirect impact on immune cell attraction and activation. Furthermore, mast cells affect adipose tissue remodelling and fibrosis by adipocyte differentiation, fibroblast proliferation and enhancing extracellular matrix proteins expression. This review will summarize current knowledge on mast cell features and their role in the development of chronic low‐grade inflammation within adipose tissue.