bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Thermodynamic Study of Aqueous 1‐Allyl‐3‐Methylimidazolium Formate Ionic Liquid as a Tailored Sorbent for Carbon Dioxide Separation
Authors: Xie Yujiao , Raut Dilip G. , Samikannu Rakesh , Mikkola Jyri‐Pekka , Ji Xiaoyan 
Published: February 2017

AbstractIn this work, aqueous 1‐allyl‐3‐methylimidazolium formate ([Amim][HCOO]) was studied as a potential sorbent for CO2 separation. The density and viscosity of aqueous [Amim][HCOO] were measured at temperatures ranging from 293.15 to 333.15 K at atmospheric pressure. The solubility of CO2 and CH4 in dry [Amim][HCOO] as well as the CO2 solubility in aqueous [Amim][HCOO] were measured at pressures up to 1.8 MPa and temperatures of 298.2, 313.2, and 333.2 K. The results showed that the density and viscosity of aqueous [Amim][HCOO] as well as the CO2 solubility in aqueous [Amim][HCOO] decreased upon increasing the water concentration and temperature. The viscosity was very sensitive to the water concentration. The experimental density and viscosity of aqueous [Amim][HCOO] were fitted to semiempirical equations, and the excess molar volume and viscosity deviations were calculated to investigate the interaction between the [Amim][HCOO] ionic liquid and water. The experimental vapor–liquid equilibrium was represented with the nonrandom two‐liquid and Redlich–Kwong model. The model parameters can be further implemented into Aspen Plus software to conduct process simulations.
A multistate population‐based analysis of linked maternal and neonatal discharge records to identify risk factors for neonatal brachial plexus injury
Authors: Freeman Michael D. , Goodyear Shaun M. , Leith Wendy M. 
Published: February 2017

AbstractObjectiveTo evaluate the interaction and contribution of maternal and fetal risk factors associated with neonatal brachial plexus injury (BPI).MethodsIn a case–control study, matched maternal and neonatal discharge records were accessed from US State Inpatient Databases for New Jersey (2010–2012), Michigan (2010–2011), and Hawaii (2010–2011). Univariate and multivariate logistic regressions were used to evaluate associations between risk factors and BPI. Area under the receiver operating characteristic curve was used to build predictive models, including two stratified models evaluating deliveries among obese and diabetic cohorts.ResultsAmong 376 325 deliveries, BPI was diagnosed in 274 (0.1%). Significant BPI risk factors included maternal obesity (odds ratio [OR] 2.7, 95% confidence interval [CI] 1.7–4.4), maternal diabetes (OR 4.6, 95% CI 3.0–7.0), use of forceps (OR 4.6, 95% CI 2.3–9.0), and vacuum assistance (OR 2.3, 95% CI 1.7–3.3). After adjusting for shoulder dystocia and other predictive factors, cesarean reduced the risk of BPI by 88% (OR 0.1, 95% CI 0.07–0.2). When stratified by obesity and diabetes, the ORs for BPI increased significantly for macrosomia, forceps, and vacuum assistance.ConclusionThe analysis confirms and quantifies more precisely the impact of risk factors for neonatal BPI, and provides a reliable basis for evidence‐based clinical decision‐making models.
The clinical implementation of primary HPV screening
Authors: Mariani Luciano , Igidbashian Sarah , Sandri Maria Teresa , Vici Patrizia , Landoni Fabio 
Published: February 2017

AbstractObjectiveTo evaluate, from a gynecology perspective, the transition from cytology‐based HPV screening to primary HPV screening.MethodsStudies examining switching from cytology‐based screening to primary HPV‐DNA testing with triaging of patients with positive test results were retrieved and reviewed, with a particular focus on screening in an Italian setting.ResultsThe increased complexity of patient‐management decisions when implementing HPV‐based screening was a critical issue discussed in the literature. The change in strategy represents a paradigm shift in moving from a medical perspective of identifying the disease in individual patients, to a public‐healthcare perspective of excluding HPV from the healthy population and identifying a small sub‐group of individuals at increased risk of HPV.ConclusionWith knowledge about HPV screening evolving rapidly, new programs and related algorithms need to be sufficiently flexible to be adjusted according to ongoing research and the validation of new assays. The establishment of a national working group (including epidemiologists, gynecologists, pathologists, and healthcare providers) will be necessary to properly implement and govern this important technical and cultural transition.
Angiogenesis mediators in women with idiopathic heavy menstrual bleeding
Authors: Elkilani Osama A. , Soliman Mohamed A. 
Published: February 2017

AbstractObjectiveTo assess the relationship between stromal cell‐derived factor 1 (SDF‐1) and mature endothelial cells in patients with heavy menstrual bleeding (HMB).MethodsIn a prospective observational study, women with idiopathic HMB and control individuals attending Menoufia University Hospital, Egypt, between August 2015 and January 2016 were enrolled. The inclusion criteria were a regular menstrual cycle, a normal coagulation study, and no anomalous ultrasonographic or hysteroscopic findings. Blood samples were collected during different phases of the menstrual cycle (day 5, ovulation, day 24) for measurement of the SDF‐1 plasma level (by enzyme‐linked immunosorbent assay) and for quantification of mature endothelial cells (by flow cytometry).ResultsOverall, 20 women with HMB and 10 control individuals were enrolled. The SDF‐1 level was significantly lower in the HMB group than in the control group during all phases of the menstrual cycle (P≤0.05 for all). The percentage of mature endothelial cells was significantly higher in the HMB group than among controls (P<0.001 for all). The SDF‐1 level and the percentage of endothelial cells were negatively correlated throughout the cycle (P<0.001 for all).ConclusionSome mediators of angiogenesis, such as SDF‐1 and endothelial cells, are disturbed in women with idiopathic HMB.
Intimate partner violence and constraints to reproductive autonomy and reproductive health among women seeking abortion services in Bangladesh
Authors: Pearson Erin , Andersen Kathryn L. , Biswas Kamal , Chowdhury Rezwana , Sherman Susan G. , Decker Michele R. 
Published: February 2017

AbstractObjectiveTo understand intersections between intimate partner violence (IPV) and other constraints to women's reproductive autonomy, and the influence of IPV on reproductive health.MethodsA secondary analysis examined cross‐sectional data from a facility‐based sample of women seeking abortion care (for spontaneous or induced abortion) between March 1 and October 31, 2013. Women aged 18–49 years, who received abortion services and selected a short‐acting contraceptive method or no contraception completed an interviewer‐administered survey after treatment. Adjusted prevalence ratios (aPRs) were calculated for associations between IPV experience and potential constraints to reproductive autonomy and health outcomes.ResultsThere were 457 participants included in the present analysis and 118 (25.8%) had experienced IPV in the preceding year. IPV was associated with discordance in fertility intentions with husbands/partners and in‐laws, with in‐law opposition to contraception, with perceived religious prohibition of contraception, and with presenting unaccompanied (all P<0.05). IPV was also associated with receiving post‐abortion care after an induced abortion compared with accessing legal menstrual regulation, and with the use of medication abortion compared with manual vacuum aspiration (both P<0.05).ConclusionIntimate partner violence was associated with additional constraints on reproductive autonomy from husbands/partners, in‐laws, and religious communities. Seeking induced abortion unaccompanied and using medication abortion could be strategies to access abortion covertly among women experiencing IPV. Ensuring women's reproductive freedom requires addressing IPV and related constraints.
Lake Urmia Basin Drought Risk Management: A Trade‐Off Between Environment and Agriculture
Authors: Moghaddasi M. , Morid S. , Delavar M. , Hossaini Safa H. 
Published: February 2017

AbstractThe increasing severity of drought worldwide and recent environmental efforts to restore wetlands have put more pressure on management of water resources.The Lake Urmia Basin is an excellent example of this; the lake has been shrinking for the last two decades, agricultural water demand is growing and frequent drought has affected the basin negatively. The present study is part of the Lake Urmia drought risk management (DRM) that suggests a drought warning system with measures to reduce agricultural water consumption and allocate a reasonable amount of water for the lake at each drought level. The approaches to reduce agricultural demand include reductions in cropped area and deficit irrigation; these have been accepted by stakeholders and are calculated by means of optimization models. Strategies to allocate water to Lake Urmia include full allocation of 3100 MCM yr−1 (the new water allocation of the lake) or up to 65% partial allocation of this amount in accordance with the severity of drought. These policies were simulated for the agricultural and environmental sectors throughout the historical and generated time series for streamflow. The results of simulation show that the new water allocations put increasing pressure on the agricultural sector and the basin will remain under water scarcity for more than 50% of the time after applying the DRM. It is a unique way to save the lake, which will otherwise dry up within a decade. The methodology can be recommended to model components of similar drought plans which will benefit from its applicability and objectivity. Copyright © 2017 John Wiley & Sons, Ltd.
Award Winner in the Young Investigator Category, 2017 Society for Biomaterials Annual Meeting and Exposition, Minneapolis, MN, April 05—08, 2017: Lymph node stiffness‐mimicking hydrogels regulate human B‐cell lymphoma growth and cell surface receptor expression in a molecular subtype‐specific manner
Authors: Apoorva F.N.U. , Tian Ye F. , Pierpont Timothy M. , Bassen David M. , Cerchietti Leandro , Butcher Jonathan T. , Weiss Robert S. , Singh Ankur 
Published: February 2017

AbstractDiffuse large B‐cell lymphoma (DLBCL) is the most common type of non‐Hodgkin's lymphoma, with multiple molecular subtypes. The activated B‐cell‐like DLBCL subtype accounts for roughly one‐third of all the cases and has an inferior prognosis. There is a need to develop better class of therapeutics that could target molecular pathways in resistant DLBCLs; however, this requires DLBCLs to be studied in representative tumor microenvironments. The pathogenesis and progression of lymphoma has been mostly studied from the point of view of genetic alterations and intracellular pathway dysregulation. By comparison, the importance of lymphoma microenvironment in which these malignant cells arise and reside has not been studied in as much detail. We have recently elucidated the role of integrin signaling in lymphomas and demonstrated that inhibition of integrin‐ligand interactions abrogated the proliferation of malignant cells in vitro and in patient‐derived xenograft. Here we demonstrate the role of lymph node tissue stiffness on DLBCL in a B‐cell molecular subtype specific manner. We engineered tunable bioartificial hydrogels that mimicked the stiffness of healthy and neoplastic lymph nodes of a transgenic mouse model and primary human lymphoma tumors. Our results demonstrate that molecularly diverse DLBCLs grow differentially in soft and high stiffness microenvironments, which further modulates the integrin and B‐cell receptor expression level as well as response to therapeutics. We anticipate that our findings will be broadly useful to study lymphoma biology and discover new class of therapeutics that target B‐cell tumors in physical environments. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1833–1844, 2017.
Canalization Leads to Similar Whole Bone Mechanical Function at Maturity in Two Inbred Strains of Mice
Authors: Schlecht Stephen H , Smith Lauren M , Ramcharan Melissa A , Bigelow Erin MR , Nolan Bonnie T , Mathis Noah J , Cathey Amber , Manley Eugene , Menon Rajasree , McEachin Richard C , Nadeau Joseph H , Jepsen Karl J 
Published: February 2017

ABSTRACT

Previously, we showed that cortical mineralization is coordinately adjusted to mechanically offset external bone size differences between A/J (narrow) and C57BL/6J (wide) mouse femora to achieve whole bone strength equivalence at adulthood. The identity of the genes and their interactions that are responsible for establishing this homeostatic state (ie, canalization) remain unknown. We hypothesize that these inbred strains, whose interindividual differences in bone structure and material properties mimic that observed among humans, achieve functional homeostasis by differentially adjusting key molecular pathways regulating external bone size and mineralization throughout growth. The cortices of A/J and C57BL/6J male mouse femora were phenotyped and gene expression levels were assessed across growth (ie, ages 2, 4, 6, 8, 12, 16 weeks). A difference in total cross-sectional area (p &lt; 0.01) and cortical tissue mineral density were apparent between mouse strains by age 2 weeks and maintained at adulthood (p &lt; 0.01). These phenotypic dissimilarities corresponded to gene expression level differences among key regulatory pathways throughout growth. A/J mice had a 1.55- to 7.65-fold greater expression among genes inhibitory to Wnt pathway induction, whereas genes involved in cortical mineralization were largely upregulated 1.50- to 3.77-fold to compensate for their narrow diaphysis. Additionally, both mouse strains showed an upregulation among Wnt pathway antagonists corresponding to the onset of adult ambulation (ie, increased physiological loads). This contrasts with other studies showing an increase in Wnt pathway activation after functionally isolated, experimental in vivo loading regimens. A/J and C57BL/6J long bones provide a model to develop a systems-based approach to identify individual genes and the gene-gene interactions that contribute to trait differences between the strains while being involved in the process by which these traits are coordinately adjusted to establish similar levels of mechanical function, thus providing insight into the process of canalization. © 2017 American Society for Bone and Mineral Research.
A Chaperone Complex Formed by HSP47, FKBP65, and BiP Modulates Telopeptide Lysyl Hydroxylation of Type I Procollagen
Authors: Duran Ivan , Martin Jorge H , Weis Mary Ann , Krejci Pavel , Konik Peter , Li Bing , Alanay Yasemin , Lietman Caressa , Lee Brendan , Eyre David , Cohn Daniel H , Krakow Deborah 
Published: February 2017

ABSTRACTLysine hydroxylation of type I collagen telopeptides varies from tissue to tissue, and these distinct hydroxylation patterns modulate collagen cross‐linking to generate a unique extracellular matrix. Abnormalities in these patterns contribute to pathologies that include osteogenesis imperfecta (OI), fibrosis, and cancer. Telopeptide procollagen modifications are carried out by lysyl hydroxylase 2 (LH2); however, little is known regarding how this enzyme regulates hydroxylation patterns. We identified an ER complex of resident chaperones that includes HSP47, FKBP65, and BiP regulating the activity of LH2. Our findings show that FKBP65 and HSP47 modulate the activity of LH2 to either favor or repress its activity. BiP was also identified as a member of the complex, playing a role in enhancing the formation of the complex. This newly identified ER chaperone complex contributes to our understanding of how LH2 regulates lysyl hydroxylation of type I collagen C‐telopeptides to affect the quality of connective tissues. © 2017 American Society for Bone and Mineral Research.
Connexin43 Mutant Patient‐Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential
Authors: Esseltine Jessica L , Shao Qing , Brooks Courtney , Sampson Jacinda , Betts Dean H , Séguin Cheryle A , Laird Dale W 
Published: February 2017

ABSTRACTWe present for the first time the generation of induced pluripotent stem cells (iPSCs) from a patient with a connexin‐linked disease. The importance of gap junctional intercellular communication in bone homeostasis is exemplified by the autosomal dominant developmental disorder oculodentodigital dysplasia (ODDD), which is linked to mutations in the GJA1 (Cx43) gene. ODDD is characterized by craniofacial malformations, ophthalmic deficits, enamel hypoplasia, and syndactyly. In addition to harboring a Cx43 p.V216L mutation, ODDD iPSCs exhibit reduced Cx43 mRNA and protein abundance when compared to control iPSCs and display impaired channel function. Osteogenic differentiation involved an early, and dramatic downregulation of Cx43 followed by a slight upregulation during the final stages of differentiation. Interestingly, osteoblast differentiation was delayed in ODDD iPSCs. Moreover, Cx43 subcellular localization was altered during chondrogenic differentiation of ODDD iPSCs compared to controls and this may have contributed to the more compact cartilage pellet morphology found in differentiated ODDD iPSCs. These studies highlight the importance of Cx43 expression and function during osteoblast and chondrocyte differentiation, and establish a potential mechanism for how ODDD‐associated Cx43 mutations may have altered cell lineages involved in bone and cartilage development. © 2017 American Society for Bone and Mineral Research.
Serum bilirubin and the risk of rheumatoid arthritis
Authors: Juping Du , Yuan Yuan , Shiyong Chen , Jun Li , Xiuxiu Zhou , Haijian Ying , Jianfeng Shi , Bo Shen 
Published: February 2017

BackgroundOxidative stress and immune imbalance play an important role in the pathogenesis of rheumatoid arthritis (RA). Bilirubin is a powerful antioxidant and also regarded as immunomodulator. Increased evidence shows that bilirubin should be a protective factor for autoimmune disease. However, the relationship between bilirubin and RA remain unclear.MethodsWe analyzed serum bilirubin levels and other laboratory and clinical data in 130 RA patients (35 patients without any complications), 81 osteoarthritis (OA) patients and 96 healthy controls.ResultsBinary logistic regression adjusted by age and gender revealed that the levels of serum total, indirect bilirubin were significantly lower in RA patients, when compared with healthy controls (P=.015, OR=0.767, 95% CI=0.619‐0.951; P=.010, OR=0.664, 95% CI=0.487‐0.906, respectively) or OA patients (P=.000, OR=0.763, 95% CI=0.661‐0.882; P=.000, OR=0.656, 95% CI=0.532‐0.808, respectively). A reduced trend of levels of bilirubin has been detected along with increased disease activity, despite with no significance (P>.05). Spearman rank test further demonstrated that IgG and ESR were negative associated with total, indirect bilirubin, and albumin, prealbumin, APOA, HDL‐C were positively associated with bilirubin.ConclusionsIn conclusion, the levels of serum bilirubins were decreased in RA, and decreased levels could be associated with IgG, albumin and inflammatory marker ESR.
Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii
Authors: Lima Bruno P. , Shi Wenyuan , Lux Renate 
Published: February 2017

AbstractTo successfully colonize the oral cavity, bacteria must directly or indirectly adhere to available oral surfaces. Fusobacterium nucleatum plays an important role in oral biofilm community development due to its broad adherence abilities, serving as a bridge between members of the oral biofilm that cannot directly bind to each other. In our efforts to characterize the molecular mechanisms utilized by F. nucleatum to physically bind to key members of the oral community, we investigated the involvement of F. nucleatum outer membrane proteins in its ability to bind to the pioneer biofilm colonizer, Streptococcus gordonii. Here, we present evidence that in addition to the previously characterized fusobacterial adhesin RadD, the interaction between F. nucleatum ATCC 23726 and S. gordonii V288 involves a second outer membrane protein, which we named coaggregation mediating protein A (CmpA). We also characterized the role of CmpA in dual‐species biofilm formation with S. gordonii V288, evaluated growth‐phase‐dependent as well as biofilm expression profiles of radD and cmpA, and confirmed an important role for CmpA, especially under biofilm growth conditions. Our findings underscore the complex set of specific interactions involved in physical binding and thus community integration of interacting bacterial species. This complex set of interactions could have critical implications for the formation and maturation of the oral biofilms in vivo, and could provide clues to the mechanism behind the distribution of organisms inside the human oral cavity.
Single‐nucleotide substitution T to A in the polypyrimidine stretch at the splice acceptor site of intron 9 causes exon 10 skipping in the ACAT1 gene
Authors: Sasai Hideo , Aoyama Yuka , Otsuka Hiroki , Abdelkreem Elsayed , Nakama Mina , Hori Tomohiro , Ohnishi Hidenori , Turner Lesley , Fukao Toshiyuki 
Published: February 2017

AbstractBackgroundβ‐ketothiolase (T2, gene symbol ACAT1) deficiency is an autosomal recessive disorder, affecting isoleucine and ketone body metabolism. We encountered a patient (GK03) with T2 deficiency whose T2 mRNA level was <10% of the control, but in whom a previous routine cDNA analysis had failed to find any mutations. Genomic PCR‐direct sequencing showed homozygosity for c.941‐9T>A in the polypyrimidine stretch at the splice acceptor site of intron 9 of ACAT1. Initially, we regarded this variant as not being disease‐causing by a method of predicting the effect of splicing using in silico tools. However, based on other findings of exon 10 splicing, we eventually hypothesized that this mutation causes exon 10 skipping.MethodscDNA analysis was performed using GK03's fibroblasts treated with/without cycloheximide (CHX), since exon 10 skipping caused a frameshift and nonsense‐mediated mRNA decay (NMD). Minigene splicing experiment was done to confirm aberrant splicing.ResultscDNA analysis using fibroblasts cultured with cycloheximide indeed showed the occurrence of exon 10 skipping. A minigene splicing experiment clearly showed that the c.941‐9T>A mutant resulted in transcripts with exon 10 skipping. There are few reports describing that single‐nucleotide substitutions in polypyrimidine stretches of splice acceptor sites cause aberrant splicing.ConclusionWe showed that c.941‐9T>A induces aberrant splicing in the ACAT1 gene. Our ability to predict the effects of mutations on splicing using in silico tools is still limited. cDNA analysis and minigene splicing experiments remain useful alternatives to reveal splice defects.