bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes of Retinal Surgery Performed by Supervised First-Year Vitreoretinal Fellows-in-Training
Authors: Levinson Joshua D. , Lai Michael M. , Daubert Jacquelyn 
Published: June 2017

Purpose: To review the outcomes of vitreoretinal surgery (VRS) performed by supervised first-year fellows-in-training. Methods: A retrospective review was performed of consecutive VRS cases performed by 4 consecutive first-year fellows under direct attending supervision in a tertiary-care teaching hospital. Main outcomes were rates of surgical complications including vitreous hemorrhage (VH) and retinal detachment (RD). Results: One hundred thirty-five cases were reviewed. Common indications for surgery included rhegmatogenous retinal detachment (RRD, 31.9%), VH (26.7%), tractional retinal detachment (TRD, 25.9%), and combined TRD/RRD (5.2%). The mean logarithm of the minimal angle of resolution visual acuity improved from preoperative 1.48 to 0.98 ( P < .0001). Forty-three cases were performed for RRD repair. Grade C proliferative vitreoretinopathy was present preoperatively in 34.9% (15 of 43). The mean duration of symptoms prior to RD repair was 40.1 days. The primary reattachment rate was 81.4% (35 of 43). Sixty-six vitrectomies were performed for proliferative diabetic retinopathy. Eleven (16.7%) patients experienced early VH, and 9 (13.6%) had delayed VH. Postoperative RD occurred in 6 (9.1%) patients all of whom initially presented with TRD. Outcomes of surgeries performed by beginning fellows (months 1-4) were compared to those performed by more experienced first-year fellows (months 5-12). Complication rates were found to be similar between the groups. Conclusion: With appropriate supervision, VRS can be safely performed by beginning fellows-in-training. Despite complex pathology, surgical outcomes of the first-year fellow cases compare favorably to published results.
Prognostic value of plasma DPP4 activity in ST-elevation myocardial infarction
Authors: Li Jing-Wei , Chen Yun-Dai , Chen Wei-Ren , You Qi , Li Bo , Zhou Hao , Zhang Ying , Han Tian-Wen 
Published: June 2017

Abstract
Background
Dipeptidyl peptidase-4 (DPP4) regulates blood glucose levels and inflammation, and it is also implicated in the pathophysiological process of myocardial infarction (MI). Plasma DPP4 activity (DPP4a) may provide prognostic information regarding outcomes for ST-segment elevation MI (STEMI) patients.

Methods
Blood samples were obtained from 625 consecutively admitted, percutaneous coronary intervention-treated STEMI patients with a mean age of 57 years old. DPP4a was quantified using enzymatic assays.

Results
The median follow-up period was 30 months. Multivariate Cox-regression analyses (adjusted for confounding variables) showed that a 1 U/L increase of DPP4a did not associate with risks of major adverse cardiac or cerebrovascular events (MACCE), cardiovascular mortality, MI, heart failure readmission, stroke, non-cardiovascular mortality and repeated revascularization. However, in a subset of 149 diabetic STEMI patients, DPP4a associated with an increased risk of MACCE (HR 1.16; 95% CI 1.04–1.30; p = 0.01).

Conclusions
DPP4a did not associate with cardiovascular events and non-cardiovascular mortality in non-diabetic STEMI patients. However, DPP4a may be associated with future MACCE in diabetic STEMI patients.
Trial registration NCT03046576, registered on 5 February, 2017, retrospectively registered
Vascular sprouts induce local attraction of proangiogenic neutrophils
Authors: Christoffersson Gustaf , Lomei Jalal , O'Callaghan Paul , Kreuger Johan , Engblom Stefan , Phillipson Mia 
Published: June 2017

Abstract
Angiogenesis, the growth of new blood vessels, is a complex process requiring the orchestration of numerous different cell types, growth factors, and chemokines. Some of the recently acknowledged actors in this process are immune cells. They accumulate at hypoxic sites, but the kinetics, dynamics, and regulation of that trafficking are unknown. In this study, we used intravital and live cell imaging to understand how neutrophils and macrophages migrate and behave at angiogenic sites. We developed two reproducible models of angiogenesis: one by transplanting isolated and hypoxic pancreatic islets into the cremaster muscles of mice, and another by in vitro coculturing of mouse aortic rings with neutrophils. In vivo imaging of the hypoxic site revealed recruitment of neutrophils and macrophages, which occurred in parallel, with depletion of one subset not affecting the accumulation of the other. We found, by cell tracking and statistical analyses, that neutrophils migrated in a directional manner to “angiogenic hotspots” around the islet where endothelial sprouting occurs, which was confirmed in the in vitro model of angiogenesis and is dependent on CXCL12 signaling. Intimate interactions between neutrophils and neovessels were prevalent, and neutrophil depletion greatly hampered vessel growth. Macrophages were less motile and attained supportive positions around the neovessels. Here, we present two novel in vivo and in vitro imaging models to study leukocyte behavior and actions during angiogenesis. These models unveiled that neutrophil migration at a hypoxic site was guided by signals emanating from sprouting endothelium where these immune cells gathered at “angiogenic hotspots” at which vascular growth occurred.
Falls, Functioning, and Disability Among Women With Persistent Symptoms of Chemotherapy-Induced Peripheral Neuropathy
Authors: Winters-Stone Kerri M. , Horak Fay , Jacobs Peter G. , Trubowitz Phoebe , Dieckmann Nathan F. , Stoyles Sydnee , Faithfull Sara 
Published: June 2017

PurposeChemotherapy-induced peripheral neuropathy (CIPN) may persist after treatment ends and may lead to functional decline and falls. This study compared objective and self-report measures of physical function, gait patterns, and falls between women cancer survivors with and without symptoms of CIPN to identify targets for functional rehabilitation.MethodsA secondary data analysis of 512 women cancer survivors (age, 62 ± 6 years; time since diagnosis, 5.8 ± 4.1 years) categorized and compared women self-reporting symptoms of CIPN (CIPN+) with asymptomatic women (CIPN−) on the following: maximal leg strength, timed chair stand, physical function battery, gait characteristics (speed; step number, rate, and length; base of support), self-report physical function and disability, and falls in the past year.ResultsAfter an average of 6 years after treatment, 47% of women still reported symptoms of CIPN. CIPN+ had significantly worse self-report and objectively measured function than did CIPN−, with the exception of maximal leg strength and base of support during a usual walk. Gait was slower among CIPN+, with those women taking significantly more, but slower and shorter, steps than did CIPN− (all P < .05). CIPN+ reported significantly more disability and 1.8 times the risk of falls compared with CIPN− ( P < .0001). Increasing symptom severity was linearly associated with worsening function, increasing disability, and higher fall risk (all P < .05).ConclusionThis work makes a significant contribution toward understanding the functional impact of CIPN symptoms on cancer survivors. Remarkably, 47% of women in our sample had CIPN symptoms many years after treatment, together with worse function, greater disability, and more falls. CIPN must be assessed earlier in the clinical pathway, and strategies to limit symptom progression and to improve function must be included in clinical and survivorship care plans.
Role of ERα in Mediating Female Uterine Transcriptional Responses to IGF1
Authors: Hewitt Sylvia C , Winuthayanon Wipawee , Lierz Sydney L , Hamilton Katherine J , Donoghue Lauren J , Ramsey J Tyler , Grimm Sara A , Arao Yukitomo , Korach Kenneth S 
Published: June 2017

Abstract
Estrogen (E2) signaling through its nuclear receptor, E2 receptor α (ERα) increases insulinlike growth factor 1 (IGF1) in the rodent uterus, which then initiates further signals via the IGF1 receptor. Directly administering IGF1 results in similar biological and transcriptional uterine responses. Our studies using global ERα-null mice demonstrated a loss of uterine biological responses of the uterus to E2 or IGF1 treatment, while maintaining transcriptional responses to IGF1. To address this discrepancy in the need for uterine ERα in mediating the IGF1 transcriptional vs growth responses, we assessed the IGF1 transcriptional responses in PgrCre+Esr1f/f (called ERαUtcKO) mice, which selectively lack ERα in progesterone receptor (PGR) expressing cells, including all uterine cells, while maintaining ERα expression in other tissues and cells that do not express Pgr. Additionally, we profiled IGF1-induced ERα binding sites in uterine chromatin using chromatin immunoprecipitation sequencing. Herein, we explore the transcriptional and molecular signaling that underlies our findings to refine our understanding of uterine IGF1 signaling and identify ERα-mediated and ERα-independent uterine transcriptional responses. Defining these mechanisms in vivo in whole tissue and animal contexts provides details of nuclear receptor mediated mechanisms that impact biological systems and have potential applicability to reproductive processes of humans, livestock and wildlife.
Extracellular polyphosphate signals through Ras and Akt to prime Dictyostelium discoideum cells for development
Authors: Suess Patrick M. , Watson Jacob , Chen Wensheng , Gomer Richard H. 
Published: June 2017

ABSTRACT
Linear chains of five to hundreds of phosphates called polyphosphate are found in organisms ranging from bacteria to humans, but their function is poorly understood. In Dictyostelium discoideum, polyphosphate is used as a secreted signal that inhibits cytokinesis in an autocrine negative feedback loop. To elucidate how cells respond to this unusual signal, we undertook a proteomic analysis of cells treated with physiological levels of polyphosphate and observed that polyphosphate causes cells to decrease levels of actin cytoskeleton proteins, possibly explaining how polyphosphate inhibits cytokinesis. Polyphosphate also causes proteasome protein levels to decrease, and in both Dictyostelium and human leukemia cells, decreases proteasome activity and cell proliferation. Polyphosphate also induces Dictyostelium cells to begin development by increasing expression of the cell–cell adhesion molecule CsA (also known as CsaA) and causing aggregation, and this effect, as well as the inhibition of proteasome activity, is mediated by Ras and Akt proteins. Surprisingly, Ras and Akt do not affect the ability of polyphosphate to inhibit proliferation, suggesting that a branching pathway mediates the effects of polyphosphate, with one branch affecting proliferation, and the other branch affecting development.
Quantifying Double McCormick
Authors: Speakman Emily , Lee Jon 
Published: June 2017

When using the standard McCormick inequalities twice to convexify trilinear monomials, as is often the practice in modeling and software, there is a choice of which variables to group first. For the important case in which the domain is a nonnegative box, we calculate the volume of the resulting relaxation, as a function of the bounds defining the box. In this manner, we precisely quantify the strength of the different possible relaxations defined by all three groupings, in addition to the trilinear hull itself. As a by-product, we characterize the best double-McCormick relaxation. We wish to emphasize that, in the context of spatial branch and bound for factorable formulations, our results do not only apply to variables in the input formulation. Our results apply to monomials that involve auxiliary variables as well. So, our results apply to the product of any three (possibly complicated) expressions in a formulation.
A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens
Authors: Wang Honghui , Drake Steven K , Yong Chen , Gucek Marjan , Lyes Matthew A , Rosenberg Avi Z , Soderblom Erik , Arthur Moseley M , Dekker John P , Suffredini Anthony F 
Published: June 2017

Abstract

BACKGROUND
Rapid identification of respiratory pathogens may facilitate targeted antimicrobial therapy. Direct identification of bacteria in bronchoalveolar lavage (BAL) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is confounded by interfering substances. We describe a method to identify unique peptide markers of 5 gram-negative bacteria by liquid chromatography–tandem mass spectrometry (LC-MS/MS) for direct pathogen identification in BAL.

METHODS
In silico translation and digestion were performed on 14–25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae. Peptides constituting theoretical core peptidomes in each were identified. Rapid tryptic digestion was performed; peptides were analyzed by LC-MS/MS and compared with the theoretical core peptidomes. High-confidence core peptides (false discovery rate &lt;1%) were identified and analyzed with the lowest common ancestor search to yield potential species-specific peptide markers. The species specificity of each peptide was verified with protein BLAST. Further, 1 or 2 pathogens were serially diluted into pooled inflamed BAL, and a targeted LC-MS/MS assay was used to detect 25 peptides simultaneously.

RESULTS
Five unique peptides with the highest abundance for each pathogen distinguished these pathogens with varied detection sensitivities. Peptide markers for A. baumannii and P. aeruginosa, when spiked simultaneously into inflamed BAL, were detected with as few as 3.6 (0.2) × 103 and 2.2 (0.6) × 103 colony-forming units, respectively, by targeted LC-MS/MS.

CONCLUSIONS
This proof-of-concept study shows the feasibility of identifying unique peptides in BAL for 5 gram-negative bacterial pathogens, and it may provide a novel approach for rapid direct identification of bacterial pathogens in BAL.
Whole-Genome Sequence and Variant Analysis of W303, a Widely-Used Strain of Saccharomyces cerevisiae
Authors: Matheson Kinnari , Parsons Lance , Gammie Alison 
Published: June 2017

Abstract
The yeast Saccharomyces cerevisiae has emerged as a superior model organism. Selection of distinct laboratory strains of S. cerevisiae with unique phenotypic properties, such as superior mating or sporulation efficiencies, has facilitated advancements in research. W303 is one such laboratory strain that is closely related to the first completely sequenced yeast strain, S288C. In this work, we provide a high-quality, annotated genome sequence for W303 for utilization in comparative analyses and genome-wide studies. Approximately 9500 variations exist between S288C and W303, affecting the protein sequences of ∼700 genes. A listing of the polymorphisms and divergent genes is provided for researchers interested in identifying the genetic basis for phenotypic differences between W303 and S288C. Several divergent functional gene families were identified, including flocculation and sporulation genes, likely representing selection for desirable laboratory phenotypes. Interestingly, remnants of ancestor wine strains were found on several chromosomes. Finally, as a test of the utility of the high-quality reference genome, variant mapping revealed more accurate identification of accumulated mutations in passaged mismatch repair-defective strains.
Whole Genome Sequencing of the Braconid Parasitoid Wasp Fopius arisanus, an Important Biocontrol Agent of Pest Tepritid Fruit Flies
Authors: Geib Scott M , Liang Guang Hong , Murphy Terence D , Sim Sheina B 
Published: June 2017

Abstract
The braconid wasp Fopius arisanus (Sonan) is an important biological control agent of tropical and subtropical pest fruit flies, including two important global pests, the Mediterranean fruit fly (Ceratitis capitata), and the oriental fruit fly (Bactrocera dorsalis). The goal of this study was to develop foundational genomic resources for this species to provide tools that can be used to answer questions exploring the multitrophic interactions between the host and parasitoid in this important research system. Here, we present a whole genome assembly of F. arisanus, derived from a pool of haploid offspring from a single unmated female. The genome is ∼154 Mb in size, with a N50 contig and scaffold size of 51,867 bp and 0.98 Mb, respectively. Utilizing existing RNA-Seq data for this species, as well as publicly available peptide sequences from related Hymenoptera, a high quality gene annotation set, which includes 10,991 protein coding genes, was generated. Prior to this assembly submission, no RefSeq proteins were present for this species. Parasitic wasps play an important role in a diverse ecosystem as well as a role in biological control of agricultural pests. This whole genome assembly and annotation data represents the first genome-scale assembly for this species or any closely related Opiine, and are publicly available in the National Center for Biotechnology Information Genome and RefSeq databases, providing a much needed genomic resource for this hymenopteran group.
Occupational violence in pregnant women in Brazil: a sample of cases in the Labor Court
Authors: Turatti Bárbara de Oliveira , Moretti-Pires Rodrigo Otávio 
Published: June 2017

Abstract
Brazilian women are still a recurring target of discrimination in the workplace, facing violence related to gender relations and moral harassment, especially when they are pregnant. When the worker perceives discriminatory acts and attitudes or any violation of the rights guaranteed by law, she may appeal to the Labor Court to initiate legal action. This in turn exposes the worker to a number of issues, such as workplace persecution and future dismissal. The rights of pregnant women to temporary stability, free time for medical examinations, change of duties and maternity leave contrast with the usurpation of the administration’s workforce. The rights of pregnant women to temporary stability, free time for medical examinations, change of duties and maternity leave contrast with the growing power of labor administration.
The Yale Observation Scale Score and the Risk of Serious Bacterial Infections in Febrile Infants
Authors: Nigrovic Lise E. , Mahajan Prashant V. , Blumberg Stephen M. , Browne Lorin R. , Linakis James G. , Ruddy Richard M. , Bennett Jonathan E. , Rogers Alexander J. , Tzimenatos Leah , Powell Elizabeth C. , Alpern Elizabeth R. , Casper T. Charles , Ramilo Octavio , Kuppermann Nathan ,   for the Febrile Infant Working Group of the Pediatric Emergency Care Applied Research Network (PECARN)
Published: June 2017

OBJECTIVES:To assess the performance of the Yale Observation Scale (YOS) score and unstructured clinician suspicion to identify febrile infants ≤60 days of age with and without serious bacterial infections (SBIs).METHODS:We performed a planned secondary analysis of a prospective cohort of non–critically ill, febrile, full-term infants ≤60 days of age presenting to 1 of 26 participating emergency departments in the Pediatric Emergency Care Applied Research Network. We defined SBIs as urinary tract infections, bacteremia, or bacterial meningitis, with the latter 2 considered invasive bacterial infections. Emergency department clinicians applied the YOS (range: 6–30; normal score: ≤10) and estimated the risk of SBI using unstructured clinician suspicion (&lt;1%, 1%–5%, 6%–10%, 11%–50%, or &gt;50%).RESULTS:Of the 4591 eligible infants, 444 (9.7%) had SBIs and 97 (2.1%) had invasive bacterial infections. Of the 4058 infants with YOS scores of ≤10, 388 (9.6%) had SBIs (sensitivity: 51/439 [11.6%]; 95% confidence interval [CI]: 8.8%–15.0%; negative predictive value: 3670/4058 [90.4%]; 95% CI: 89.5%–91.3%) and 72 (1.8%) had invasive bacterial infections (sensitivity 23/95 [24.2%], 95% CI: 16.0%–34.1%; negative predictive value: 3983/4055 [98.2%], 95% CI: 97.8%–98.6%). Of the infants with clinician suspicion of &lt;1%, 106 had SBIs (6.4%) and 16 (1.0%) had invasive bacterial infections.CONCLUSIONS:In this large prospective cohort of febrile infants ≤60 days of age, neither the YOS score nor unstructured clinician suspicion reliably identified those with invasive bacterial infections. More accurate clinical and laboratory predictors are needed to risk stratify febrile infants.
Evaluation of tetrakis(diethylamino)hafnium Precursor in the Formation of Hafnium Oxide Films Using Atomic Layer Deposition
Authors: Inman Ronald , Deshpande Anand , Jursich Gregory 
Published: June 2017

AbstractDue to their compatibility with silicon interface and high dielectric constant, films containing hafnium oxide are becoming strong candidates in replacing silicon oxynitride as the gate dielectric layer in CMOS devices. To achieve ultimate conformality and thickness control, atomic layer deposition is receiving much more attention in recent years for nanometer size film applications. For hafnium oxide deposition by ALD, metal chlorides have traditionally been used as precursors with moisture being the co-reactant; however for gate oxide applications, metal chlorides are not considered suitable due to the corrosive nature of these compounds and the risks of film contamination. Hence, researchers are exploring alternate organometallic precursors in a CVD process with oxygen being the co-reactant. In this work, tetrakis (diethylamino) hafnium precursor is used in an ALD process with moisture co-reactant to deposit hafnium oxide films onto H-terminated Si substrate in a temperature regime of 200 to 350 C. Film composition is determined by x-ray analysis and is found to be stoichiometric without residue from ligand decomposition. Film thickness and uniformity is measured as a function of substrate temperature and reagent pulsing characteristics. These results will be presented and compared with that obtained with the more conventional hafnium chloride precursor.
The Prs2O3 /Si(001) Interface: a Mixed Si-Pr Oxide
Authors: Schmeißer Dieter , Dabrowski Jarek , Müssig Hans-Joachim 
Published: June 2017

AbstractWe studied the Pr2O3/Si(001) interface by a non-destructive depth profiling using synchrotron radiation and photo-electron spectroscopy (SR-PES) at the undulator beam line U49/2-PGM2 and ab initio calculations. Our results provide evidence that a chemical reactive interface exists consisting of a mixed Si-Pr oxide such as (Pr2O3)(SiO)x(SiO2)y. There is no formation of neither an interfacial SiO2 nor interfacial silicide: all Si-Pr bonds are oxidized and all SiO4 units dissolve in the Pr oxide. Under ultrahigh vacuum conditions, silicide formation is observed only when the film is heated above 800°C in vacuum. Interfacial silicates like (Pr2O3)(SiO)x(SiO2)y are promising high-k dielectric materials, e.g., because they represent incremental modification of SiO2 films by Pr ions, so that the interface characteristics can be similar to Si-SiO2 interface properties. The Pr silicate system formed in a natural way at the interface between Si(001) and Pr2O3 offers an increased flexibility towards integration of Pr2O3 into future CMOS technologies.
Atomic Layer Deposition of Hafnium Oxide Thin Films from Tetrakis(dimethylamino)Hafnium (TDMAH) and Ozone
Authors: Liu Xinye , Ramanathan Sasangan , Seidel Thomas E. 
Published: June 2017

AbstractHafnium oxide (HfO2) thin films were synthesized from tetrakis(dimethylamino) hafnium (TDMAH) and ozone (O3) by atomic layer deposition (ALD) on 200 mm silicon wafers. Gradual saturation was observed for TDMAH exposure pulse. However O3 showed better saturation behavior for O3exposure. Yet, 100% step coverage was achieved for ~100nm trenches with aspect ratio of 35. Temperature dependence of the deposition rate was studied at susceptor temperature from 160°C to 420°C. The lowest deposition rate was observed at 320°C. Mercury probe measurements indicated the dielectric constant increased from 16 to 20 as susceptor temperature increased from 200°C to 320°C. Selected comparisons with tetrakis (ethylmethylamino) hafnium (TEMAH) were also made.