bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jing‐Si Herbal Tea Suppresses H2O2‐Instigated Inflammation and Apoptosis by Inhibiting Bax and Mitochondrial Cytochrome C Release in HIG‐82 Synoviocytes
Authors: Kao Shih‐Wen , Chang Yu‐Chun , Lin Feng‐Huei , Huang Tai‐Lung , Chen Tung‐Sheng , Lin Shinn‐Zong , Lin Kuan‐Ho , Kuo Wei‐Wen , Ho Tsung‐Jung , Huang Chih‐Yang 
Published: September 2024

ABSTRACTInflammation is an intrinsic protective mechanism against various forms of cellular injuries in humans; however, its undesired activation results in tissue damage and cell death. The onset of chronic inflammation and oxidative stress are the key characteristics of autoimmune inflammatory diseases such as rheumatoid arthritis (RA), for which an effective treatment is yet to be developed. Therefore, in this study, we investigated the protective effects and molecular mechanisms of a novel herbal preparation, Jing‐Si herbal tea (JS), against H2O2‐induced inflammation and cellular damage in HIG‐82 synoviocytes. We found that JS did not show any significant alterations in cell viability at <188 μg/mL; however, a cytotoxic effect was observed at 188–1883 μg/mL concentrations tested. We found that expressions of inflammation associated extracellular matrix (ECM)‐degrading proteases MMP‐13, ADAMTS‐2, ‐8, and ‐17 were abnormally enhanced under H2O2‐induced pathological oxidative stress (ROS) in HIG‐82 cells. Interestingly, JS treatment not only reduced the ROS levels but also significantly repressed the protein expressions of collagen degrading proteases in a dose‐dependent manner. Treatment with JS showed enhanced cell viability against H2O2‐induced toxic ROS levels. The expressions of cell protective aggrecan, Collagen II, and Bcl‐2 were increased, whereas MMP‐13, ADAMTS‐2, Cytochrome C, and cleaved Caspase 3 were decreased by JS under inflammatory agents H2O2, MIA, LPS, and TNF‐α treatment, respectively, in HIG‐82 cells. Interestingly, the cytoprotective effect of JS treatment was attributed to a decreased mitochondrial localization of Bax and a reduction of Cytochrome C release into the cytoplasm of H2O2‐treated HIG‐82 cells. Collectively, our results suggest a novel protective mechanism of JS for RA treatment, which could be potentially applied as a complementary treatment or as an alternative therapeutic approach to mitigate inflammatory diseases.
How reliable is the X‐ray fluorescence‐based differentiation between glass wool and rock wool and the age classification of rock wool?
Authors: Paul Andrea , Liestmann Zoe , Zaenker Steffen , Vogel Kristin , Broszies Tanja , Ostermann Markus 
Published: September 2024

AbstractA prerequisite for the recycling of mineral wool is information about the type of material, that is, whether it is glass wool or rock wool. As mineral wool produced before the year 2000 can be potentially carcinogenic, it is furthermore important to distinguish between “old” and “new” wool when handling the material. Based on VDI 3492, it is possible to determine the material and, in the case of rock wool, the age by analyzing the mass fractions of eight oxides, which are the main components of mineral wool. This study presents the X‐ray fluorescence (XRF) analyses of 141 man‐made mineral fibers collected in Germany. Only in a few cases it was not possible to clearly assign the material type. In contrast, the identification of “old” and “new” rock wool posed a challenge as there were many borderline samples. Based on the available data, a chemometric model was developed that can classify “old” and “new” RW with a sensitivity of 93% and 89% and with a specificity of 100% in both cases. However, care must be taken when oxide contents are close to the specification limits. The reason for this mainly lies in the overlapping intervals of key oxides as suggested by VDI 3492, and, to a lesser extent, in the uncertainties typically occurring in the XRF‐based analysis of oxides. With this study, a comprehensive collection and evaluation of XRF data on mineral wool is made available, which can serve as a reference database for future users.
Localizability of the approximation method
Authors: Pich Ján 
Published: September 2024

AbstractWe use the approximation method of Razborov to analyze the locality barrier which arose from the investigation of the hardness magnification approach to complexity lower bounds. Adapting a limitation of the approximation method obtained by Razborov, we show that in many cases it is not possible to combine the approximation method with typical (localizable) hardness magnification theorems to derive strong circuit lower bounds. In particular, one cannot use the approximation method to derive an extremely strong constant-depth circuit lower bound and then magnify it to an $$\textsf{NC}^{1}$$

NC
1

lower bound for an explicit function. To prove this, we show that lower bounds obtained by the approximation method are in many cases localizable in the sense that they imply lower bounds for circuits which are allowed to use arbitrarily powerful oracles with small fan-in.
Revolutionizing transportation: an overview of 3D printing in aviation, automotive, and space industries
Authors: Wawryniuk Zuzanna , Brancewicz-Steinmetz Emila , Sawicki Jacek 
Published: September 2024

AbstractThis review article provides a deep dive into the diverse landscape of Additive Manufacturing (AM) technologies and their significant impact on the automotive and aviation sectors. It starts by exploring various AM methodologies such as Fused Deposition Modeling (FDM), Stereolithography (SLA), Digital Light Processing (DLP), Selective Laser Sintering (SLS), Metal Jet Fusion (MJF), Binder Jetting (BJ), and Directed Energy Deposition (DED), with a specific focus on their applicability, strengths, and challenges within these industries. The article then delves into the practical applications of AM in rapid prototyping, functional part production, and component repair. The results highlight the versatility and precision of SLA and DLP, the strength and durability of SLS, and the potential of metal-based technologies like LPBF, SLM, EBM, and DMLS in manufacturing critical components. The integration of AM with automotive and aviation design underscores the transformative nature of these technologies, driving advancements in lightweight, intricate, and high-performance components. The review concludes by emphasising AM's significant opportunities and acknowledging the ongoing challenges in material properties, post-processing, and production scalability, thereby underscoring the necessity for future research and innovation in these sectors.
Characterization of pre- and on-treatment soluble immune mediators and the tumor microenvironment in NSCLC patients receiving PD-1/L1 inhibitor monotherapy
Authors: Murata Daiki , Azuma Koichi , Murotani Kenta , Kawahara Akihiko , Nishii Yuuya , Tokito Takaaki , Sasada Tetsuro , Hoshino Tomoaki 
Published: September 2024

Abstract
Background
Despite the favorable therapeutic efficacy observed with ICI monotherapy, the majority of non-small cell lung cancer (NSCLC) patients do not respond. Therefore, identifying patients who could optimally benefit from ICI treatment remains a challenge.

Methods
Among 183 patients with advanced or recurrent NSCLC who received ICI monotherapy, we analyzed 110 patients whose pre- and post-treatment plasma samples were available. Seventy-three soluble immune mediators were measured at ICI initiation and 6 weeks later. To identify useful biomarkers, we analyzed the association of pre-treatment levels and on-treatment changes of soluble immune mediators with survival of patients. The associations of pre-treatment or on-treatment biomarkers with irAE development, PD-L1 expression, CD8+ TIL density, and neutrophil to lymphocyte ratio (NLR) were also analyzed.

Results
Univariate analysis showed that pre-treatment biomarkers included 6 immune mediators, whereas on-treatment biomarkers included 8 immune mediators. Multivariate analysis showed that pre-treatment biomarkers included 4 immune mediators (CCL19, CCL21, CXCL5, CXCL10), whereas on-treatment biomarkers included 5 immune mediators (CCL7, CCL19, CCL23, CCL25, IL-32). IrAE development was associated with on-treatment change in CCL23. PD-L1 expression was associated with the pre-treatment levels of TNFSF13B and the on-treatment change in CCL25. CD8+ TIL density was associated with the pre-treatment CXCL10 level, whereas NLR was correlated with pre-treatment levels of CCL13 and CCL17.

Conclusion
We identified several soluble immune mediators as pre-treatment and on-treatment biomarkers of survival in patients with NSCLC treated with ICI monotherapy. Some of these biomarkers were associated with other possible predictors, including irAE development, PD-L1 expression, CD8+ TIL density and NLR. Further large-scale studies are needed to establish biomarkers for patients with NSCLC who received ICI monotherapy.
Transcriptional activation domains interact with ATPase subunits of yeast chromatin remodelling complexes SWI/SNF, RSC and INO80
Authors: Wendegatz Eva-Carina , Engelhardt Maike , Schüller Hans-Joachim 
Published: September 2024

AbstractChromatin remodelling complexes (CRC) are ATP-dependent molecular machines important for the dynamic organization of nucleosomes along eukaryotic DNA. CRCs SWI/SNF, RSC and INO80 can move positioned nucleosomes in promoter DNA, leading to nucleosome-depleted regions which facilitate access of general transcription factors. This function is strongly supported by transcriptional activators being able to interact with subunits of various CRCs. In this work we show that SWI/SNF subunits Swi1, Swi2, Snf5 and Snf6 can bind to activation domains of Ino2 required for expression of phospholipid biosynthetic genes in yeast. We identify an activator binding domain (ABD) of ATPase Swi2 and show that this ABD is functionally dispensable, presumably because ABDs of other SWI/SNF subunits can compensate for the loss. In contrast, mutational characterization of the ABD of the Swi2-related ATPase Sth1 revealed that some conserved basic and hydrophobic amino acids within this domain are essential for the function of Sth1. While ABDs of Swi2 and Sth1 define separate functional protein domains, mapping of an ABD within ATPase Ino80 showed co-localization with its HSA domain also required for binding actin-related proteins. Comparative interaction studies finally demonstrated that several unrelated activators each exhibit a specific binding pattern with ABDs of Swi2, Sth1 and Ino80.
The interaction of lipomatous hypertrophy of the interatrial septum with pericardial adipose tissue biomarkers by computed tomography
Authors: Lacaita Pietro G. , Senoner Thomas , Bilgeri Valentin , Rauch Stefan , Barbieri Fabian , Kindl Benedikt , Plank Fabian , Dichtl Wolfgang , Deeg Johannes , Widmann Gerlig , Feuchtner Gudrun M. 
Published: September 2024

Abstract
Objective
Novel pericardial adipose tissue imaging biomarkers are currently under investigation for cardiovascular risk stratification. However, a specific compartment of the epicardial adipose tissue (EAT), lipomatous hypertrophy of the interatrial septum (LHIS), is included in the pericardial fat volume (PCFV) quantification software. Our aim was to evaluate LHIS by computed tomography angiography (CTA), to elaborate differences to other pericardial adipose tissue components (EAT) and paracardial adipose tissue (PAT), and to compare CT with [18F]FDG-PET.

Materials and methods
Of 6983 patients screened who underwent coronary CTA for clinical indications, 190 patients with LHIS were finally included (age 62.8 years ± 9.6, 31.6% females, BMI 28.5 kg/cm2 ± 4.7) in our retrospective cohort study. CT images were quantified for LHIS, EAT, and PAT density (HU), and total PCFV, with and without LHIS, was calculated. CT was compared with [18F]FDG-PET if available.

Results
CT-density of LHIS was higher (− 22.4 HU ± 22.8) than all other pericardial adipose tissue components: EAT right and left (97.4 HU ± 13 and − 95.1 HU ± 13) PAT right and left (− 107.5 HU ± 13.4 and − 106.3 HU ± 14.5) and PCFV density −83.3 HU ± 5.6 (p < 0.001). There was a mild association between LHIS and PAT right (Beta 0.338, p = 0.006, 95% CI: 0.098–577) and PAT left (Beta 0.249, p = 0.030; 95% CI: 0.024–0.474) but not EAT right (p = 0.325) and left (p = 0.351), and not with total PCFV density (p = 0.164). The segmented LHIS volume comprised 3.01% of the total PCFV, and 4.3% (range, 2.16–11.7%) in those with LHIS > 9 mm. [18F]FDG-PET: LHIS was tracer uptake positive in 83.3% (37.5%: mild and 45.8%: minimal) of 24 patients.

Conclusions
LHIS is a distinct compartment of PCFV with higher density suggesting brown fat and has no consistent association with EAT, but rather with PAT.

Clinical relevance statement
LHIS should be recognized as a distinct compartment of the EAT, when using EAT for cardiovascular risk stratification.

Key Points

LHIS is currently included in EAT quantification software.

LHIS density is relatively high, it is not associated with EAT, and has a high [18F]FDG-PET positive rate suggesting brown fat.

LHIS is a distinct compartment of the EAT, and it may act differently as an imaging biomarker for cardiovascular risk stratification.
Myxospore density of Kudoa inornata varies significantly within symmetrical white muscle tissue replicates of its fish host, the spotted seatrout, Cynoscion nebulosus
Authors: Snyder Augustus M. , McElroy Eric J. , de Buron Isaure , Casu Fabio , Beers Jody M. 
Published: September 2024

AbstractThe spotted seatrout, Cynoscion nebulosus, is a popular game fish in the southeastern USA. It is estimated that nearly 90% of the adult population in South Carolina estuaries are infected in their skeletal muscle by the myxosporean, Kudoa inornata. However, little is known about this parasite’s biology, including the distribution and densities of myxospores within tissues of infected fish, which we expect affect the physiology of their hosts. In order to correlate densities with physiological parameters in future studies, we quantified the myxospores density in muscle and characterized the variation among individual fish. Naïve juvenile seatrout was experimentally infected via presumed K. inornata actinospores exposure to raw seawater. A plug of muscle was extracted from two bilaterally symmetrical regions in the epaxial fillet from fresh and frozen carcasses. Variation in density data was calculated both within and among individuals. Within individuals, density counts were compared between left- and right-side biopsies. There was no significant difference between fresh and frozen plugs, and variation among individuals accounted for the greatest proportion of variation at 68.8%, while variation within individuals was substantial at 25.6%. Simulation and correlation tests confirmed that bilaterally symmetrical replicates varied significantly within individuals. When sampled from areas surrounding the initial biopsies, myxospore density estimates were more similar than between sides. Our findings have important implications for sampling design, particularly for studies investigating physiological parameters at the cellular or molecular level in association with parasite infection.
Switch to faricimab after initial treatment with aflibercept in eyes with neovascular age-related macular degeneration
Authors: Aldhanhani Aisha A. , Azzam Ola A. , AlAli Sahar H. , Almasri Khaled G. , Aljneibi Shaikha H. , Pichi Francesco 
Published: September 2024

Abstract
Purpose
To investigate the efficacy and outcomes of switching neovascular age-related macular degeneration (nAMD) patients from aflibercept to faricimab, focusing on visual acuity, retinal fluid management, and treatment intervals. The primary aim was to assess the early outcomes in nAMD patients refractory to aflibercept and explore faricimab’s potential as a longer-lasting therapeutic alternative.

Methods
A single-center retrospective study was conducted on 50 refractory nAMD patients at Cleveland Clinic Abu Dhabi from September 2022–May 2023. Patients were switched from aflibercept to faricimab, having met specific criteria for refractory nAMD. The study analyzed best-corrected visual acuity (BCVA), central subfield thickness (CST), and fluid changes post-switch, using Optical Coherence Tomography (OCT).

Results
After three faricimab injections, significant reductions in CST were observed, with a notable decrease in retinal fluid. The mean BCVA remained stable throughout the study period. Although there was a decrease in the maximum pigment epithelial detachment (PED) height, it was not statistically significant. Treatment intervals post-switch showed that the majority of patients maintained or extended their treatment intervals, with a significant proportion achieving resolution of intraretinal fluid (IRF) and subretinal fluid (SRF).

Conclusions
Switching to faricimab from aflibercept in refractory nAMD patients led to significant improvements in retinal fluid management and CST, with stable BCVA outcomes. Faricimab presents a promising alternative for patients requiring frequent aflibercept injections, potentially offering a more manageable treatment regimen with extended dosing intervals. This study highlights the need for personalized therapeutic strategies in nAMD treatment, though further research is necessary to optimize treatment switches.
Survey of Recent Results in Privacy-Preserving Mechanisms for Multi-Agent Systems
Authors: Kossek Magdalena , Stefanovic Margareta 
Published: September 2024

AbstractPrivacy-preserving communication in cooperative control is essential for effective operations of various systems where sensitive information needs to be protected. This includes systems such as smart grids, traffic management systems, autonomous vehicle networks, healthcare systems, financial networks, and social networks. Recent privacy-preserving cooperative control literature is categorized and discussed in this paper. Advantages and disadvantages of differential privacy and encryption-based privacy-preserving protocols are described. The objective of this work is to examine and analyze existing research and knowledge related to the preservation of privacy in the context of cooperative control. This paper aims to identify and present a range of approaches, techniques, and methodologies that have been proposed or employed to address privacy concerns in multi-agent systems. It seeks to explore the current challenges, limitations, and gaps in the existing literature. It also aims to consolidate the findings from various studies to provide an overview of privacy-preserving cooperative control in multi-agent systems. The goal is to assist in the development of novel privacy-preserving mechanisms for cooperative control.
Chondrule Properties and Formation Conditions
Authors: Marrocchi Yves , Jones Rhian H. , Russell Sara S. , Hezel Dominik C. , Barosch Jens , Kuznetsova Aleksandra 
Published: September 2024

AbstractChondrules are iconic sub-millimeter spheroids representing the most abundant high-temperature dust formed during the evolution of the circumsolar disk. Chondrules have been the subject of a great deal of research, but no consensus has yet emerged as to their formation conditions. In particular, the question of whether chondrules are of nebular or planetary origin remains largely debated. Building upon decades of chondrule investigation and recent headways in combining petrographic observations and O−Ti−Cr isotopic compositions, we here propose a comprehensive vision of chondrule formation. This holistic approach points toward a nebular origin of both NC and CC chondrules, with repetitive high-temperature recycling processes controlling the petrographic and isotopic diversities shown by chondrules. Chondrule precursors correspond to mixing between (i) early-formed refractory inclusions ± NC-like dust and (ii) previous generation of chondrules ± CI-like material. Chondrule formation took place under open conditions with gas-melt interactions with multi-species gas (H2O, Mg, SiO) playing a key role for establishing their characteristics. Petrographic and isotopic systematics do not support disk-wide transport of chondrules but point toward local formation of chondrules within their respective accretion reservoirs. Altogether, this shows that several generations of genetically-related chondrules (i.e., deriving from each other) co-exist in chondrites. In addition to supporting the nebular brand of chondrule-forming scenarios, this argues for repetitive and extremely localized heating events for producing chondrules.
Reconciling conflicting sustainability rationalities: a co-creation approach in urban logistics governance
Authors: Shrestha Subina 
Published: September 2024

AbstractTransforming urban logistics governance to become more sustainable comprises reconciling diverse actors’ rationalities. Yet, conventional market-driven solutions, which aim to optimize freight transport, tend to focus on operational efficiency over diverse rationalities. In this paper, we reframe the challenge of urban logistics sustainability governance as that of knowledge integration, which necessitates a transdisciplinary approach that engages with diverse rationalities. By combining data from a co-creation workshop and interviews with logistics actors, we investigate if and how urban logistics sustainability governance can benefit from unpacking these multiple rationalities. Our findings show emergent tensions in Bergen stem from the ad-hoc and fragmented nature of urban logistics planning, which obscures the diverse actor rationalities and assumptions. Actors navigated these tensions in dialogic processes and co-created a shared understanding that sustaining dialogues and using a public-space perspective could mainstream logistics into the city’s planning process. Adopting a transdisciplinary co-creation approach, we demonstrate, can reconcile the diverse rationalities in urban logistics sustainability governance. It facilitates individual and social learning in dialogic processes where actors can reflect on each other’s perspectives, agency, and expectations. We thus call for a shift in planning from an emphasis on market-driven solutions toward process-focus to navigate the innate messiness of governing urban logistics sustainability.
Lattice Parameter of Austenite in Silicon Cast Irons
Authors: Lacaze Jacques , Lopez Marcos G. , Dehmas Moukrane 
Published: September 2024

AbstractUpon solidification, graphitic cast irons undergo a volume change whose amplitude depends on two opposite terms, the contraction associated with austenite formation and the expansion due to graphite crystallization. During cooling after solidification, further precipitation of graphite occurs that continuously changes the physical properties of the material and possibly affects the eutectoid transformation that transforms the matrix from austenitic to ferritic or ferritic-pearlitic. This work intended to study the density of graphitic cast irons at high temperature, i.e., in the temperature range where the matrix is austenitic. High-temperature laboratory X-rays have been carried out on several alloys containing various carbon and silicon contents to characterize the austenite mean lattice parameter. By complementing these results with literature data, a statistical analysis was carried out that expresses the austenite mean lattice parameter as a function of temperature and composition, evidencing the high uncertainty related to the austenite carbon content. Finally, one of the investigated alloys was submitted to a simultaneous dilatometry and X-ray analysis in a synchrotron from room temperature to 1050 °C. The data are used to discuss the austenite lattice parameter prediction and the possibility of density prediction.