bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical Sensor based on Indium Tin Oxide Glass Modified with Poly(Ethyleneimine)/Phosphomolybdic Acid Composite Multilayers
Authors: Hao Yanjun , Feng Shun , Liu Yumei , Xu Jie , Ma Yuhua , Wang Jide 
Published: January 2017

AbstractIn this work, one novel electrochemical sensor was prepared by alternative deposition of phosphomolybdic acid (PMoA) and poly(ethyleneimine) (PEI) on an indium tin oxide glass substrate through layer‐by‐layer assembly. The performance of as‐prepared electrode was evaluated with both of oxidizing compounds of iodate and H2O2 and reducing compounds of dopamine and ascorbic acid as models. The results showed that corresponding current response of redox peak increased linearly with the concentration of above compounds increasing in certain ranges, respectively. Limits of detection to them were in the range of 1.0×10−4 ‐ 4.3×10−4 mg mL−1 with cyclic voltammetry (CV) in 0.1 mol L−1 NaAc‐HAc buffer (pH 5.0). The electrode showed high stability and remained 95 % of its initial activity even after 100 cycles of CV scan. When applied in real samples of table salts, juice and human serum, high recoveries of 96.84 to 100.33 % were achieved with relative standard deviations of 1.11‐3.96 % (n=3) at three spiked levels. Moreover, it was also successfully applied for the simultaneous determination of dopamine and ascorbic acid in human serum with differential pulse voltammetry. The results indicated that PMoA/PEI multilayer modified electrode can be used as a universal electrochemical sensor for sensitive detection of redox compounds.
Comparative Electrochemical Study of N‐, C‐terminal and Integral Centrin on Adsorption and Metal‐Binding Properties
Authors: Rong Zhijiang , Zhao Yaqin , Shi Enxian , Zhang Wenlong , Yang Binsheng 
Published: January 2017

AbstractThe adsorption behavior of apo‐ciliate Euplotes octocarinatus centrin (EoCen) and N(C)‐terminal domain of EoCen (N(C)‐EoCen) at a glassy carbon (GC) electrode is studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Interestingly, the adsorption isotherms of C‐EoCen, N‐EoCen and EoCen at a GC surface differ from one another. It is considered to be associated with the different aggregation properties of three proteins. Furthermore, we analyze the metal‐binding properties of centrin and the followed changes in protein structure upon metal‐binding. Corresponding to the four binding sites of EoCen, it shows four no‐equiv signals of CV or EIS change. It indicates that the four different binding sites of EoCen can be discriminated by the EIS titration curves, which is in contrast with conventional use of spectral method. According to the electrostatic potential at the molecular surface of proteins, the favored orientation of N‐EoCen and C‐EoCen on the GC surface is modeled. These models can well explain the results of titration curves of Eu3+ to N(C)‐EoCen. This work has established the electrochemical methodology which can be used to measure metal‐binding sites involved and reveal the contribution of each metal site to the whole protein conformational change.
The interventional relationship between frequent fish consumption and depression symptoms in aging adults: a randomized controlled trial
Authors: Sharifan Payam , Hosseini Motahare‐Sadat , Sharifan Anoosheh 
Published: January 2017

ObjectiveThe present investigation was intended to test the hypothesis that the elderly provided with the frequent consumption of fishes marinated in essential oil of Perilla frutescens (EOPF) or not would experience fewer depressive symptoms after 6 months.MethodsA total of 180 participants were recruited from Sina Hospital, Mashhad, Iran, who were diagnosed with depression based on Diagnostic and Statistical Manual of Mental Disorders, 4th edition, Text Revision and Beck Depression Inventory. Participants (n = 180) were randomly assigned in a 1:1:1 ratio to Groups A, B, and C. The last two were provided with an instruction to consume Caspian white fish marinated in the presence or absence of EOPF (434 g each week or four meals per week). Group A served as the control with the common diet. The outcome measures were performed using the Beck Depression Inventory and the General Health Questionnaire.ResultsThere were no statistically significant differences in depressive symptom scores between groups with frequent fish consumption as compared with the control (p > 0.05). Yet adjustment for covariates showed that there was a significant reduction in depression among them (p < 0.05). Moreover, consumption of fish and EOPF was associated with more considerable improvements than Groups A and B (p < 0.05).ConclusionsIt could be concluded that high intakes of unsaturated fatty acids can afford to diminish likelihood of late‐life depression. Copyright © 2017 John Wiley & Sons, Ltd.
An improved method for single flow direction calculation in grid digital elevation models
Authors: Shin Sanghoon , Paik Kyungrock 
Published: January 2017

AbstractThis paper presents improvements to the global D8 (GD8) method for calculating single flow directions in a grid digital elevation model. Flow directions computed from grid digital elevation models serve as the foundation for much of the analysis and modeling of hydrological processes that are driven by topographic gradients. The literature includes both single flow direction methods, where flow goes to only one downslope cell, and multiple flow direction methods that apportion flow among multiple downslope cells. Among single flow direction methods, the standard D8 method, in which the flow direction is set based on the steepest local slope, results in bias on surfaces that do not align with the grid directions. Efforts to address this problem have led to the development of extended methods that account for elevation values further upslope in determining flow directions. We have identified discrepancies in one such method, GD8, and have examined ways to resolve these discrepancies. An improvement to GD8, named iGD8, is presented that allows replacing a reference cell from which path deviations are accumulated and that considers horizontal path deviation rather than global slope as a flow direction criterion. The improved method is found to be effective in resolving the problems encountered with GD8 and to be more efficient than a previously proposed alternative method (least transversal deviation (LTD) based D8, namely D8‐LTD) that uses recursive searching for the largest upstream area when multiple flow paths converge. The proposed improved GD8 method offers the opportunity for improved analysis and modeling of topographically driven hydrological processes by providing better foundational flow directions for these analyses.
Tumour cell conditioned medium reveals greater M2 skewing of macrophages in the absence of properdin
Authors: Al‐Rayahi Izzat A.M. , Browning Michael J. , Stover Cordula 
Published: January 2017

AbstractIntroductionThe tumour microenvironment is shaped by the interaction of immune, non immune, and tumour cells present in close proximity. Tumour cells direct the development of a locally immune suppressed state, affecting the activity of anti tumour T cells and preparing the escape phase of tumour development. Macrophages in the tumour typically develop into so‐called tumour associated macrophages with a distinct profile of activities which lead to a reduction in inflammation and antigen presentation. The direct impact of tumour cell conditioned medium on the activity profile of macrophages in dependence of their complement component expression has not yet been investigated.MethodsIn our in vitro study, macrophages differentiated from bone marrows of properdin deficient and wildtype mice were stimulated with conditioned medium of a syngeneic tumour cell line, B16F10, a mouse melanoma subline.ResultsIn comparison with macrophages from wildtype mice, those from congenic properdin deficient mice showed skewing towards M2 profile, encompassing mRNA expression for genes involved in arginine metabolism, production of type 2 cytokines, and relatively lower surface expression of molecules needed for antigen presentation.ConclusionsThese data suggest that properdin insufficiency promotes a tumour environment that helps the tumour evade the immune response.
The dental resin monomers HEMA and TEGDMA have inhibitory effects on osteoclast differentiation with low cytotoxicity
Authors: Inamitsu Hiroyuki , Okamoto Kuniaki , Sakai Eiko , Nishishita Kazuhisa , Murata Hiroshi , Tsukuba Takayuki 
Published: January 2017

AbstractThe dental resin monomers 2‐hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) are released from the resin matrix due to unpolymerized monomers; once released, they influence various biological functions and the viability of cells in the oral environment. Although HEMA and TEGDMA have various effects on cells, including inflammation, inhibition of cell proliferation or differentiation, and apoptosis, the effects of these monomers on osteoclasts remain unknown. In this study, we investigated the effects of HEMA and TEGDMA on osteoclast differentiation of bone marrow‐derived macrophages or murine monocytic cell line RAW‐D. Both HEMA and TEGDMA inhibited osteoclast formation and their bone‐resorbing activity at non‐cytotoxic concentrations. Moreover, HEMA and TEGDMA decreased the expression of nuclear factor of activated T cells cytoplasmic‐1 (NFATc1), a master regulator of osteoclast differentiation, and of osteoclast markers that are transcriptionally regulated by NFATc1, including Src and cathepsin K. Regarding their effects on signaling pathways involved in osteoclast differentiation, HEMA impaired the phosphorylation of extracellular signal‐regulated kinase and Jun N‐terminal kinase, whereas TEGDMA attenuated the phosphorylation of Akt and Jun N‐terminal kinase. Thus, HEMA and TEGDMA inhibit osteoclast differentiation through different signaling pathways. This is the first report on the effects of the monomers HEMA and TEGDMA on osteoclasts. Copyright © 2017 John Wiley & Sons, Ltd.
Vacuum ultraviolet photon–mediated production of [18F]F2
Authors: Krzyczmonik Anna , Keller Thomas , Kirjavainen Anna K. , Forsback Sarita , Solin Olof 
Published: January 2017

The chemistry of F2 and its derivatives are amenable to facile aliphatic or aromatic substitution, as well as electrophilic addition. The main limitation in the use of [18F]F2 for radiopharmaceutical synthesis is the low specific activity achieved by the traditional methods of production. The highest specific activities, 55 GBq/μmol, for [18F]F2 have been achieved so far by using electrical discharge in the post‐target production of [18F]F2 gas from [18F]CH3F. We demonstrate that [18F]F2 is produced by illuminating a gas mixture of neon/F2/[18F]CH3F with vacuum ultraviolet photons generated by an excimer laser. We tested several illumination chambers and production conditions. The effects of the initial amount of [18F]F‐, amount of carrier F2, and number of 193‐nm laser pulses at constant power were evaluated regarding radiochemical yield and specific activity. The specific activity attained for [18F]F2‐derived [18F]NFSi was 10.3 ± 0.9 GBq/μmol, and the average radiochemical yield over a wide range of conditions was 6.7% from [18F]F‐. The production can be improved by optimization of the synthesis device and procedures. The use of a commercially available excimer laser and the simplicity of the process can make this method relatively easy for adaptation in radiochemistry laboratories.
Confirming therapeutic target of protopine using immobilized β2‐adrenoceptor coupled with site‐directed molecular docking and the target‐drug interaction by frontal analysis and injection amount–dependent method
Authors: Liu Guangxin , Wang Pei , Li Chan , Wang Jing , Sun Zhenyu , Zhao Xinfeng , Zheng Xiaohui 
Published: January 2017

AbstractDrug‐protein interaction analysis is pregnant in designing new leads during drug discovery. We prepared the stationary phase containing immobilized β2‐adrenoceptor (β2‐AR) by linkage of the receptor on macroporous silica gel surface through N,N′‐carbonyldiimidazole method. The stationary phase was applied in identifying antiasthmatic target of protopine guided by the prediction of site‐directed molecular docking. Subsequent application of immobilized β2‐AR in exploring the binding of protopine to the receptor was realized by frontal analysis and injection amount–dependent method. The association constants of protopine to β2‐AR by the 2 methods were (1.00 ± 0.06) × 105M−1 and (1.52 ± 0.14) × 104M−1. The numbers of binding sites were (1.23 ± 0.07) × 10−7M and (9.09 ± 0.06) × 10−7M, respectively. These results indicated that β2‐AR is the specific target for therapeutic action of protopine in vivo. The target‐drug binding occurred on Ser169 in crystal structure of the receptor. Compared with frontal analysis, injection amount–dependent method is advantageous to drug saving, improvement of sampling efficiency, and performing speed. It has grave potential in high‐throughput drug‐receptor interaction analysis.
Modeling Tree Loss Versus Tree Recruitment Processes in SW Iberian Rangelands as Influenced by Topography and Land use and Management
Authors: Herguido Estela , Lavado Contador Joaquín Francisco , Gómez Gutiérrez Álvaro , Schnabel Susanne 
Published: January 2017

AbstractThe sustainability of Iberian open wooded rangelands is threatened by recent land use changes and lack of tree recruitment. As trees are key elements of these systems, the characterization of their spatiotemporal trend is essential for the system's management. Our objective is to develop spatial models reflecting the temporal dynamic of trees in terms of recent tree loss and tree gain processes in farms, evaluating the influence on the models of topography and land use and management. Aerial photographs of 1956 and 2009 were compared, analyzing lost and new trees in five dehesas of Extremadura (Spain). Multivariate adaptive regression splines was used to produce the spatial models that characterize the proneness of an area to undergo tree loss or recruitment. Models showed good performances. Although land use and management intensively influenced on tree dynamics, because it depends on topography, models built by considering just the topographic variables showed only slightly lower fitness. Tree recruitment mostly happened in the marginal places with steep slopes, while tree loss occurred in elevated, gently undulated and more intensively used places of the farms. A spatial polarization of both processes has been observed that leads to the lack of replacement of lost trees. The work provides some clues on the design of tree management procedures for dehesas and montados. The implementation of measures to address the degradation processes in dehesas should take into account the spatial arrangement of the areas where tree loss or gain is actually happening or it is likely to occur. Copyright © 2017 John Wiley & Sons, Ltd.
A novel de novo frameshift deletion in EHMT1 in a patient with Kleefstra Syndrome results in decreased H3K9 dimethylation
Authors: Blackburn Patrick R. , Williams Monique , Cousin Margot A. , Boczek Nicole J. , Beek Geoffrey J. , Lomberk Gwen A. , Urrutia Raul A. , Babovic‐Vuksanovic Dusica , Klee Eric W. 
Published: January 2017

AbstractBackgroundKleefstra Syndrome (KS) (MIM# 610253) is an autosomal dominant disorder caused by haploinsufficiency of euchromatic histone methyltransferase‐1 (EHMT1, GLP). EHMT1 (MIM# 607001) encodes a histone methyltransferase that heterodimerizes with EHMT2 (also known as G9a, MIM# 604599), which together are responsible for mono‐ and dimethylation of H3 lysine 9 (H3K9me1 and ‐me2), resulting in transcriptional repression of target genes.MethodsThis report describes an 18‐year‐old woman with intellectual disability, severely limited speech, hypotonia, microcephaly, and facial dysmorphisms, who was found to have a novel de novo single‐base frameshift deletion in EHMT1.ResultsFunctional studies using patient fibroblasts showed decreased H3K9me2 compared to wild‐type control cells, thus providing a rapid confirmatory test that complements molecular studies.ConclusionWhole exome sequencing revealed a novel frameshift deletion in EHMT1 after a lengthy diagnostic odyssey in this patient. Functional testing using this patient's fibroblasts provides proof‐of‐concept for the analysis of variants of uncertain significance that are predicted to impact EHMT1 enzymatic activity.
Wideband circularly polarized his backed fan‐shaped antenna with directional patterns covering L1‐L5 GPS bands
Authors: Babakhani Behrouz , Sharma Satish K. , Mishra Ghanshyam 
Published: January 2017

ABSTRACTA wideband circularly polarized (CP) fan‐shaped antenna employing a sequential phase feed network (SPFN) covering L1 to L5 global positioning system (GPS) bands is presented. The antenna geometry consists of two crossed bow‐tie shaped dipoles with four arms which offers bidirectional CP radiation. A SPFN feeds dipole arms with equal amplitudes and 0°, 90°, 180° and 270° phase values. To achieve directional patterns, the bidirectional antenna is backed by a mushroom‐like electromagnetic band gap (EBG) structure which serves as wideband high impedance surface (HIS). Measurement verifies that the HIS backed antenna offers acceptable impedance matching, axial ratio (AR), antenna efficiency, gain and pattern performance between 1170 MHZ to 1630 MHZ (around 32% fractional bandwidth). The measured broadside (θ = 0°) AR is below 3 dB from 1.15 GHz to 1.7 GHz (38% bandwidth). The directional RHCP measured de‐embedded gain is 5.8 dBic, 6.1 dBic, 5.5 dBic and 4.6 dBic at 1170 GHz, 1220 GHz, 1380 GHz and 1570 GHz, respectively. The de‐embedded antenna efficiency is better than 60% between 1.15 GHz and 1.6 GHz with the maximum of 90% at 1.4 GHz. The measured and simulation results agree very well. © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:497–500, 2017
Co‐design of two‐way doherty power amplifier and filter for concurrent dual‐band application
Authors: Dai Zhijiang , He Songbai , Peng Jun , Pang Jingzhou , Hao Peng , Huang Chaoyi 
Published: January 2017

ABSTRACTIn this letter, an architecture is presented to solve problems of classical concurrent dual‐band Doherty power amplifiers (DPAs) and its design procedure is given. It is a codesign of two single‐band DPAs and four filters. Two of filters at the output port are used for impedance transformation, harmonic components suppressing and signal isolating, while the another two split concurrent dual‐band signals at the input port. In this way, intermodulation components of the two bands can be effectively suppressed and each band can achieve a good performance as single‐band one's. This design concept is demonstrated through implementing of a practical dual‐band DPA operating at 1.8 and 2.5 GHz. Measurement results show that efficiencies are 65.0 and 56.3% at the 6 dB output power back‐off for concurrent application. The intermodulation components and harmonic components are lower than −40 dB except second harmonic component at 1.8 GHz (−32 dB). © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:530–533, 2017
Magnetic resonance wireless power transfer for laptop computer with a ground plane
Authors: Nguyen Van Thuan , Kang Seok Hyon , Jung Chang Won 
Published: January 2017

ABSTRACTThis paper proposes a new method to increase the transfer efficiency of a wireless power transfer (WPT) system, having a source coil, a sprial transmitter (Tx) coil, a load coil and a ground plane, by utilizing the ground plane itself. The creation of cross slits at the ground center and employment of a lumped capacitor on the ground plane transform the ground plane into an additional resonator, which makes the original WPT system work as a 4‐coil WPT system, producing a higher efficiency. By experiment, with an optimized capacitor on the ground plane of C = 2180 pF, the measurement of transfer efficiency shows a maximum improvement of 10% at a transfer distance of 40 cm, compared to the case without the ground plane. The transfer efficiency of the proposed system at transfer distance of 15 cm is 46%. Circuit theory and electromagnetic simulations are employed to analyze the proposed WPT system, giving results consistent with the measured ones. The operating frequency of the proposed system is 6.78 MHz. © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:514–521, 2017
High gain microstrip antenna array for 60 GHz band point to point WLAN/WPAN communications
Authors: Rabbani M.S. , Ghafouri‐Shiraz H. 
Published: January 2017

ABSTRACTA microstrip patch antenna array has been designed with 24 ultra‐wide patch elements to improve the antenna gain up to around 20 dBi for 60 GHz band point to point wireless local area network (WLAN) and wireless personal area network (WPAN) applications. Both series and parallel feeding techniques have been collaborated to excite the patch elements. Reverse impedance matching method has been adopted to enhance the antenna's fabrication tolerance. The measured and simulation results showed a close agreement. © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:511–514, 2017
Electromagnetic scattering from inhomogeneous objects embedded in spherically multilayered media solved by the method of moments
Authors: Chen Yongjin , Han Feng , Liu Na , Wen Paiju , Liu Qinghuo 
Published: January 2017

ABSTRACTThe method of moments (MOM) is applied to solve electromagnetic (EM) scattering problems in spherically multilayered media. A spectral‐domain dyadic Green's function (DGF) in a spherically multilayered medium is constructed in terms of the spherical vector wave functions by using the method of scattering superposition in the spherical coordinate system. Its expression is later transformed to that in the Cartesian coordinate. We discretize the computational domain which contains the scatterer into N size‐independent cells in the Cartesian coordinate. Based on the transformed DGFs, the volume integral equations can be solved by MOM combined with Krylov subspace iterative methods. In this letter, we choose the bi‐conjugate gradient stabilized (BCGS) iteration method due to its fast convergence. Numerical results compared with FDTD solutions from a commercial software are presented to validate the accuracy and efficiency of our method. © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:526–530, 2017
An electrically small sicrr metamaterial‐inspired dual‐band antenna for WLAN and WiMAX applications
Authors: Sharma Sameer Kumar , Abdalla Mahmoud A. , Chaudhary Raghvendra Kumar 
Published: January 2017

ABSTRACTThis study presents the design of an electrically small metamaterial antenna with dual‐band characteristics for WLAN and WiMAX applications with coplanar ground. The proposed antenna consists of stepped‐impedance closed ring resonator (SICRR) and a small rectangular patch which is designed by etching a split ring resonator (SRR) from a rectangular patch which is turn fed through main feed. Such metamaterial composite exhibits dual‐band behavior with first band operating at 2.45 GHz with an impedance bandwidth (|S11| < −10 dB) of 8.16% while second band is resonant at 3.37 GHz having an impedance bandwidth of 23.74% respectively. The proposed antenna is designed specifically to operate at WLAN (2.5 GHz) and WiMAX (3.5 GHz) and shows compact nature with a radiating element size of 0.16λo × 0.097λo × 0.013λo. Equivalent circuit of proposed antenna has also been modeled which is in good agreement with the antenna behavior. The proposed structure shows end fire radiation pattern in H‐plane and omni‐directional radiation pattern in E‐plane at all operating frequencies with low cross polarization levels. The prototype has been fabricated and experimentally tested to validate the simulation results which are in good agreement with the measured results. The antenna is suitable for use in Bluetooth, WLAN and WiMAX. © 2017 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:573–578, 2017