bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of keratin 19‐positive cancer stem cells associating human hepatocellular carcinoma using CYFRA 21‐1
Authors: Kawai Takayuki , Yasuchika Kentaro , Ishii Takamichi , Katayama Hokahiro , Yoshitoshi Elena Yukie , Ogiso Satoshi , Minami Takahito , Miyauchi Yuya , Kojima Hidenobu , Yamaoka Ryoya , Kita Sadahiko , Yasuda Katsutaro , Sasaki Naoya , Fukumitsu Ken , Hatano Etsuro , Uemoto Shinji 
Published: October 2017

AbstractThe current lack of an easily measurable surrogate marker of cancer stem cells (CSCs) prevents the clinical application of CSCs for hepatocellular carcinoma (HCC). We previously reported that keratin 19 (K19) is a novel HCC‐CSC marker associated with transforming growth factor beta (TGFβ)/Smad signaling, and that K19+ HCC‐CSCs could be a new therapeutic target of TGFβ receptor 1 inhibitor LY2157299. In this study, we examined whether K19+ HCC‐CSCs can be tracked using cytokeratin 19 fragment CYFRA 21‐1. In 147 HCC patients who underwent curative resection and evaluated K19 expression by immunohistochemistry, preoperative serum CYFRA 21‐1 levels were significantly higher in K19+ patients than in K19− patients (P < 0.01). Receiver operating characteristic analyses revealed that serum CYFRA 21‐1 was the statistically significant and the most sensitive predictor of tumor K19 expression among preoperative laboratory test values (P < 0.001). In HCC cells encoding with a K19 promoter‐driven enhanced green fluorescent protein, fluorescence‐activated cell sorting (FACS)‐isolated K19+ cells displayed significantly higher levels of supernatant CYFRA 21‐1 than K19− cells (P < 0.01). Gain/loss of K19 function experiments confirmed that CYFRA 21‐1 levels were regulated by K19 function in HCC cells. Furthermore, CYFRA 21‐1 levels reflected the treatment efficacy of LY2157299 in K19+ cells. In conclusion, CYFRA 21‐1 can be used to predict K19 expression in HCC, and should thereby aid in the development of novel therapeutic strategies targeting K19+ HCC‐CSCs.
Assessing Capacity to Change in High‐Risk Pregnant Women: A Pilot Study
Authors: Harnett Paul H. , Barlow Jane , Coe Chris , Newbold Caroline , Dawe Sharon 
Published: October 2017

Pre‐birth risk assessment is a process by which circumstances affecting an unborn child can be identified and support for mother and infant embedded. This mixed methods study describes a community‐based pre‐birth assessment and care pathway that utilised the Parents Under Pressure (PuP) programme to assess parenting capacity and provide support pre‐ and post‐birth for ‘at risk’ women. Sixty‐eight pregnant women referred to children's social care services were allocated to the pre‐birth assessment and care pathway (n = 35) or to routine care (n = 33). Standardised measures of psychological distress, social support and alcohol measured change for the women in the assessment and care pathway. Twenty women who provided pre‐ and post‐data reported significant improvements on all measures except alcohol use. Safeguarding outcomes at 12 months were obtained for both groups using administrative data. Forty‐two per cent of the infants whose mothers received the pre‐birth assessment and care pathway showed an improvement in child protection status compared to 14 per cent of the routine care infants. Safeguarding status deteriorated or stayed the same in 52 per cent of the routine cases compared to 26 per cent of those receiving the pathway. Qualitative data revealed that the pathway was acceptable and helpful to service users and service providers. Copyright © 2017 John Wiley & Sons, Ltd.‘Describes a community‐based pre‐birth assessment and care pathway that utilised the Parents Under Pressure (PuP) programme’Key Practitioner Messages

The current project found that a community‐based pre‐birth assessment and care pathway with high‐risk pregnant women was feasible and acceptable for practitioners and service users.
The pathway began mid‐pregnancy and support was provided following the birth of the infant for up to 12 months.
Over 40 per cent of infants whose mothers were allocated to the pre‐birth risk assessment pathway showed improvements in child safeguarding status at 12 months.
Mouse subthalamic nucleus neurons with local axon collaterals
Authors: Gouty‐Colomer Laurie‐Anne , Michel François J , Baude Agnès , Lopez‐Pauchet Catherine , Dufour Amandine , Cossart Rosa , Hammond Constance 
Published: October 2017

AbstractThe neuronal population of the subthalamic nucleus (STN) has the ability to prolong incoming cortical excitation. This could result from intra‐STN feedback excitation. The combination of inducible genetic fate mapping techniques with in vitro targeted patch‐clamp recordings, allowed identifying a new type of STN neurons that possess a highly collateralized intrinsic axon. The time window of birth dates was found to be narrow (E10.5–E14.5) with very few STN neurons born at E10.5 or E14.5. The fate mapped E11.5–12.5 STN neuronal population included 20% of neurons with profuse axonal branching inside the nucleus and a dendritic arbor that differed from that of STN neurons without local axon collaterals. They had intrinsic electrophysiological properties and in particular, the ability to generate plateau potentials, similar to that of STN neurons without local axon collaterals and more generally to that of classically described STN neurons. This suggests that a subpopulation of STN neurons forms a local glutamatergic network, which together with plateau potentials, allow amplification of hyperdirect cortical inputs and synchronization of the STN neuronal population.
Power Factor Control of Photovoltaic Generation Output for Improving Transient Stability
Authors: NOGAMI SHUN , YOKOYAMA AKIHIKO 
Published: October 2017

SUMMARYBecause of significant change in environmental policies and electric power deregulation in the last decades, a lot of photovoltaic (PV) generations have so far been installed to the power system in Japan. When a huge amount of PVs generation is installed into the power system, we are very much concerned that the disconnection of the PVs due to a system fault has a big impact on the power system stability and transient voltage stability. So it is important to equip the PV with Fault Ride Through (FRT) function in order to maintain power supply–demand balance. In addition, Dynamic Voltage Support (DVS) function of the PV is effective to recover the voltage after the system fault. However, it is unclear how the PVs which are equipped with the FRT and DVS functions affect transient stability and voltage stability. In this paper, how the power factor of the PV power output affects the power system after a system fault is analyzed using P–δ curve. Then, a new PV power factor control method for improving the transient stability is proposed and its effectiveness is shown by digital simulations on a simple power system model.
Weight Loss Trajectories in Bariatric Surgery Patients and Psychopathological Correlates
Authors: García‐Ruiz‐de‐Gordejuela Amador , Agüera Zaida , Granero Roser , Steward Trevor , Llerda‐Barberá Asunción , López‐Segura Elena , Vilarrasa Nuria , Sanchez Isabel , Jiménez‐Murcia Susana , Virgili Nuria , López‐Urdiales Rafael , de Bernabe Mónica Montserrat‐Gil , Garrido Pilar , Monseny Rosa , Monasterio Carmen , Salord Neus , Pujol‐Gebelli Jordi , Menchón Jose M. , Fernández‐Aranda Fernando 
Published: October 2017

AbstractThis study aimed to explore the empirical trajectories of body mass index (BMI) 1 year following bariatric surgery (BS) and to identify the risk factors for each trajectory. The study included 115 patients with severe obesity who underwent BS. Assessment included metabolic variables, psychopathological and personality measures. Growth mixture modelling identified four separated trajectories for the percentage of total weight loss course shape (namely, T1 ‘good‐fast’, T2 ‘good’, T3 ‘low’ and T4 ‘low‐slow’). After adjusting for BS subtype and metabolic baseline state, T1 and T2 registered less eating and general psychopathology. T1 was characterized by the lowest scores in novelty seeking and self‐transcendence, whereas T4 was defined by the highest scores in novelty seeking and the lowest scores in persistence. Our findings suggest that psychological state prior to BS is predictive of BMI trajectories during the 12 months following BS. These results could be useful in developing more efficient interventions for these patients. Copyright © 2017 John Wiley & Sons, Ltd and Eating Disorders Association.
Polydopamine‐collagen complex to enhance the biocompatibility of polydimethylsiloxane substrates for sustaining long‐term culture of L929 fibroblasts and tendon stem cells
Authors: Li Qian , Sun Lihong , Zhang Lei , Xu Zhigang , Kang Yuejun , Xue Peng 
Published: October 2017

AbstractPolydimethylsiloxane (PDMS) is a commercialized polymer extensively used in the fabrication of versatile microfluidic microdevices for studies in cell biology and tissue engineering. However, the inherent surface hydrophobicity of PDMS is not optimal for cell culture and thus restrains its applications for investigation of long‐term behaviors of fibroblasts and stem cells. To improve the surface biocompatibility of PDMS, a facile technique was developed by modifying the PDMS surface with polydopamine‐collagen (COL/PDA) complex. The successful synthesis of COL/PDA was verified through proton nuclear magnetic resonance spectroscopy. Compared to surface coating solely with COL or PDA, the surface wettability was significantly improved on COL/PDA‐modified PDMS substrates based on water contact angle characterizations. The modified PDMS surface remarkably enhanced the initial adhesion and long‐term proliferation of L929 fibroblasts and tendon stem cells (TSCs). Additionally, the effects of COL/PDA coating on cell viability and apoptosis were further investigated under prolonged incubation. We found that the COL/PDA coating on PDMS resulted in a substantial increase of cell viability compared to native PDMS, and the cell apoptosis was considerably impeded on the modified PDMS. This study demonstrated that COL/PDA coating can effectively enhance the surface biocompatibility of PDMS as verified by the enhanced adhesion and long‐term proliferation of L929 fibroblasts and TSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 408–418, 2018.
Osseous response on linear and cyclic RGD‐peptides immobilized on titanium surfacesin vitroandin vivo
Authors: Heller M. , Kumar V. V. , Pabst A. , Brieger J. , Al‐Nawas B. , Kämmerer P. W. 
Published: October 2017

AbstractBiomimetic surface modifications of titanium (Ti) implants using the Arg‐Gly‐Asp‐sequence (RGD) are promising to accelerate bone healing in cases of medical implants. Therefore, we compared the impact of linear and cyclic RGD (l‐ and c‐RGD) covalently coupled onto Ti surfaces on the osseous responsein vitroandin vivo.In vitro, osteoblasts’ behavior on different surfaces (unmodified, amino‐silanized [APTES], l‐ and c‐RGD) was analysed regarding adhesion (fluorescence microscopy), proliferation (resazurin stain) and differentiation (reverse transcription polymerase chain reaction on alkaline phosphatase and osteocalcin).In vivo, osteosynthesis screws (unmodifiedn = 8, l‐RGDn = 8, c‐RGDn = 8) were inserted into the proximal tibiae of 12 rabbits and evaluated for bone growth parameters (bone implant contact [%] and vertical bone apposition [VBA;%]) at 3 and 6 weeks.In vitro, c‐ as well as l‐RGD surfaces stimulated osteoblasts’ adherence, proliferation and differentiation in a similar manner, with only subtle evidence of superiority of the c‐RGD modifications.In vivo, c‐RGD‐modifications led to a significantly increased VBA after 3 and 6 weeks. Thus, coating with c‐RGD appears to play an important role influencing osteoblasts’ behaviourin vitrobut especiallyin vivo. These findings can be applied prospectively to implantable biomaterials with hypothetically improved survival and success rates. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 106A: 419–427, 2018.
Hydrogenolysis of glycerol to 1,2‐propanediol in a continuous flow trickle bed reactor
Authors: Manuale Debora L , Santiago Lucía V , Torres Gerardo C , Sepúlveda Jorge H , Torresi Pablo A , Vera Carlos R , Yori Juan C 
Published: October 2017

AbstractBACKGROUNDHydrogenolysis of glycerol to glycols in continuous flow three phase reactors is of practical importance due to the need to give value to huge amounts of surplus glycerol. Thermodynamic and kinetic aspects must be revised for a proper design. The system was studied in a trickle‐bed reactor using copper chromite and Cu/Al2O3as catalysts.RESULTSPhase equilibrium and flow pattern were verified. Solid, liquid and gas phases were present, with the liquid phase in ‘trickling’ flow. Catalysts were characterized by inductively coupled plasma (ICP), nitrogen sortometry, X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), temperature programmed reduction (TPR) and pyridine thermal programmed desorption (TPD). The average reaction rate was found to be practically constant under different process conditions. A theoretical analysis indicated that the resistance to the transfer of hydrogen from the gas to the liquid phase dominated the overall kinetics. Selectivity to 1,2‐propanediol varied with temperature, with a maximum at 230 °C (97%). Selectivity was a function of the catalyst acidity. When the pressure was increased the selectivity to 1,2‐propanediol was increased, up to 97% at 14 bar. Higher pressures did not modify this value.CONCLUSIONSOptimum reaction conditions for maximum selectivity to 1,2‐propanediol with Cu‐based catalysts are 230 °C and 14 bar. System kinetics are, however, dominated by the gas–liquid mass transfer resistance. © 2017 Society of Chemical Industry
Serum proteomic assessment of the progression of fracture healing
Authors: Hussein Amira I. , Mancini Christian , Lybrand Kyle E. , Cooke Margaret E. , Matheny Heather E. , Hogue Brenna L. , Tornetta Paul , Gerstenfeld Louis C. 
Published: October 2017

ABSTRACTA targeted proteomic analysis of murine serum over a 35‐day course of fracture healing was carried out to determine if serum proteomic changes could be used to monitor the biological progression of fracture healing. Transverse, closed femoral fractures where generated and stabilized with intramedullary fixation. A single stranded DNA aptamer‐based multiplexed proteomic approach was used to assay 1,310 proteins. The transcriptomic profiles for genes matching the 1,310 proteins were obtained by microarray analysis of callus mRNA. Of the 1,310 proteins analyzed, 850 proteins showed significant differences among the time points (p‐value <0.05). Ontology assessment associated these proteins with osteoblasts, monocyte/macrophage lineages, mesenchymal stem cell lines, hepatic tissues, and lymphocytes. Temporal clustering of these data identified proteins associated with inflammation, cartilage formation and bone remodeling stages of healing. VEGF, Wnt, and TGF‐βsignaling pathways were restricted to the period of cartilage formation. Comparison of the proteomic and transcriptomic profiles showed that 87.5% of proteins in serum had concordant expression to their mRNA expression in the callus, while 12.5% of the protein and mRNA expression patterns were discordant. The discordant proteins that were elevated in the serum but down regulated in callus mRNA expression were related to clotting functions, allograft rejection, and complement function. While proteins down regulated in the serum and elevated in callus mRNA were associated with osteoblast function, NF‐ĸb, and activin signaling. These data show the serum proteome may be used to monitor the different biological stages of fracture healing and have translational potential in assessing human fracture healing. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1153–1163, 2018.
Systematic review of lymphovenous anastomosis (LVA) for the treatment of lymphedema
Authors: Scaglioni Mario F. , Fontein Duveken B. Y. , Arvanitakis Michael , Giovanoli Pietro 
Published: October 2017

IntroductionLymphedema is a chronic condition caused by the obstruction or impairment of lymphatic fluid transport resulting in irreversible skin fibrosis. Besides conservative therapy, surgical techniques for lymphedema including liposuction, lymphatico‐lymphatic bypass, lymphovenous anastomosis (LVA), and vascularized lymph node transfer (VLNT) are options with increasing popularity in the recent past. In our review, we investigated the efficacy of LVA for the treatment of lymphedema. Both objective and subjective outcomes of surgical treatment were evaluated.MethodsStudies were identified through systematic review in PubMed database up to September 2016. Only original Articles which exclusively performed LVA for lymphedema treatment were included. Our primary endpoint was the objective of a subjective postoperative lymphedema reduction.ResultsA total of 293 titles were identified, out of which 18 studies including 939 patients were deemed eligible. The studies included in this review describe significant variations in surgical techniques, number of anastomoses and supplementary interventions. All studies reported objective reductions in circumference measurements. Subjective symptom relief was found in 50‐100% of the patients as well as a reduction in the number of cellulitis episodes in all investigated cases.ConclusionAlthough the studies included in this review showed great heterogeneity, LVA surgery revealed both objective and subjective improvements in most patients.
Reduced acoustic noise in diffusion tensor imaging on a compact MRI system
Authors: Tan Ek T. , Hardy Christopher J. , Shu Yunhong , In Myung‐Ho , Guidon Arnaud , Huston John , Bernstein Matt A. , K.F. Foo Thomas 
Published: October 2017

PurposeTo investigate the feasibility of substantially reducing acoustic noise while performing diffusion tensor imaging (DTI) on a compact 3T (C3T) MRI scanner equipped with a 42‐cm inner‐diameter asymmetric gradient.MethodsA‐weighted acoustic measurements were made using 10 mT/m‐amplitude sinusoidal waveforms, corresponding to echo‐planar imaging (EPI) echo spacing of 0.25 to 5.0 ms, on a conventional, whole‐body 3T MRI and on the C3T. Acoustic measurements of DTI with trapezoidal EPI waveforms were then made at peak gradient performance on the C3T (80 mT/m amplitude, 700 T/m/s slew rate) and at derated performance (33 mT/m, 10 to 50 T/m/s) for acoustic noise reduction. DTI was acquired in two different phantoms and in seven human subjects, with and without gradient‐derating corresponding to multi‐ and single‐shot acquisitions, respectively.ResultsSinusoidal waveforms on the C3T were quieter by 8.5 to 15.6 A‐weighted decibels (dBA) on average as compared to the whole‐body MRI. The derated multishot DTI acquisition noise level was only 8.7 dBA (at 13 T/m/s slew rate) above ambient, and was quieter than non‐derated, single‐shot DTI by 22.3 dBA; however, the scan time was almost quadrupled. Although derating resulted in negligible diffusivity differences in the phantoms, small biases in diffusivity measurements were observed in human subjects (apparent diffusion coefficient = +9.3 ± 8.8%, fractional anisotropy = +3.2 ± 11.2%, radial diffusivity = +9.4 ± 16.8%, parallel diffusivity = +10.3 ± 8.4%).ConclusionThe feasibility of achieving reduced acoustic noise levels with whole‐brain DTI on the C3T MRI was demonstrated. Magn Reson Med 79:2902–2911, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
Sonography‐guided recording for superficial peroneal sensory nerve conduction study
Authors: Kim Ki Hoon , Park Byung Kyu , Kim Dong Hwee , Kim Yuntae 
Published: October 2017

ABSTRACTIntroductionWe sought to establish the optimal recording position for antidromic conduction of the superficial peroneal nerve (SPN) by using ultrasonography (USG).MethodsThe sensory nerve action potentials (SNAPs) of the intermediate dorsal cutaneous nerve (IDCN) and medial dorsal cutaneous nerve (MDCN) in 64 limbs of 32 healthy participants were recorded (nerve conduction study [NCS]‐1). Both nerves were identified by using USG, and the SNAPs were obtained from the USG‐guided repositioned electrodes (NCS‐2).ResultsThe IDCN and MDCN were located at 29.3% ± 5.1% and 43.9% ± 4.9% of the intermalleolar distance from the lateral malleolus, respectively. Significantly greater amplitude was shown for SNAPs of both nerves in NCS‐2 versus NCS‐1.DiscussionThe optimal recording position is likely to be lateral, one‐third from the lateral malleolus for the IDCN, and just lateral to the midpoint of the intermalleolar line for the MDCN. When the SPN response is unexpectedly attenuated, USG‐guided repositioning of the electrodes should be considered. Muscle Nerve 57: 628–633, 2018
Exercise induced effects on muscle function and range of motion in patients with hip osteoarthritis
Authors: Bieler Theresa , Siersma Volkert , Magnusson S. Peter , Kjaer Michael , Beyer Nina 
Published: October 2017

AbstractBackground and purposePatients with hip osteoarthritis have impairments in muscle function (muscle strength and power) and hip range of motion (ROM), and it is commonly believed that effective clinical management of osteoarthritis should address these impairments to reduce pain and disability. Therefore, the purpose of this study was to compare the short‐ and long‐term effects of 4 months of physiotherapist‐supervised strength training, physiotherapist‐supervised Nordic Walking (NW), or unsupervised home‐based exercise (HBE) on muscle function and hip ROM in patients diagnosed with hip osteoarthritis.MethodsSecondary outcome analyses from an observer‐blinded three‐armed parallel‐design randomized controlled trial in 60+‐year‐old patients with clinical hip osteoarthritis (American College of Rheumatology criteria) who were not on a waiting list for hip replacement. One hundred and fifty‐two patients were randomized to either 4 months of physiotherapist‐supervised, moderate, progressive, strength training (n = 50), physiotherapist‐supervised NW (n = 50), or unsupervised HBE (n = 52). Maximal isometric hip and thigh muscle strength and leg extensor power and active hip ROM were assessed at baseline 2, 4, and 12 months.ResultsIntention‐to‐treat‐analyses did not show any significant between‐group differences for improvements in muscle strength and power or ROM at any time points. Short‐term significant (p < .05) increases in muscle strength were present in the physiotherapist‐supervised exercise groups and in the long‐term for muscle power in the NW‐group. All exercise modes resulted in significant increases of ROM but long‐term improvements were only shown for NW and HBE.DiscussionFour months of physiotherapist‐supervised, progressive, moderate, and strength training was less effective than hypothesized for improving muscle strength and power in patients with hip osteoarthritis who are not awaiting hip replacement. Our results may indicate that in these patients, improvements in disability are not necessarily dependent on improvements in strength and power or ROM.