bluuebunny/crossref_metadata_2025_split huggingface dataset:
Original Research: miR-194 inhibits proliferation and invasion and promotes apoptosis by targeting KDM5B in esophageal squamous cell carcinoma cells
Authors: Cui Guanghui , Liu Donglei , Li Weihao , Li Yuhang , Liang Youguang , Shi Wensong , Zhao Song 
Published: August 2016

Increasing evidence suggests that miR-194 is down-regulated in esophageal squamous cell carcinoma tumor tissue. However, the role and underlying mechanism of miR-194 in esophageal squamous cell carcinoma have not been well defined. We used DIANA, TargetScan and miRanda to perform target prediction analysis and found KDM5B is a potential target of miR-194. Based on these findings, we speculated that miR-194 might play a role in esophageal squamous cell carcinoma development and progression by regulation the expression of KDM5B. We detected the expression of miR-194 and KDM5B by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot assays, respectively, and found down-regulation of miR-194 and up-regulation of KDM5B existed in esophageal squamous cell carcinoma cell lines. By detecting proliferation, invasion and apoptosis of TE6 and TE14 cells transfected with miR-194 mimics or mimic control, miR-194 was found to inhibit proliferation and invasion and promote apoptosis of esophageal squamous cell carcinoma cells. miR-194 was further verified to regulate proliferation, apoptosis and invasion of esophageal squamous cell carcinoma cells by directly targeting KDM5B. Furthermore, animal studies were performed and showed that overexpression of miR-194 inhibited the growth of esophageal squamous cell carcinoma tumors in vivo. These results confirmed our speculation that miR-194 targets KDM5B to inhibit esophageal squamous cell carcinoma development and progression. These findings offer new clues for esophageal squamous cell carcinoma development and progression and novel potential therapeutic targets for esophageal squamous cell carcinoma.
Cytoprotective autophagy maintains leukemia-initiating cells in murine myeloid leukemia
Authors: Sumitomo Yoshiki , Koya Junji , Nakazaki Kumi , Kataoka Keisuke , Tsuruta-Kishino Takako , Morita Ken , Sato Tomohiko , Kurokawa Mineo 
Published: August 2016

Key Points
Autophagy is required for maintenance of AML-initiating cells and peripheral myeloblast survival. Loss of autophagy potentiates the therapeutic effects of AraC in vivo.
Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway
Authors: Li Yan , Zhang Wei , Gao Jianxin , Liu Jiaqi , Wang Hongtao , Li Jun , Yang Xuekang , He Ting , Guan Hao , Zheng Zhao , Han Shichao , Dong Maolong , Han Juntao , Shi Jihong , Hu Dahai 
Published: August 2016

Abstract
Background
Hypertrophic scars (HS) generally occur after injury to the deep layers of the dermis, resulting in functional deficiency for patients. Growing evidence has been identified that the supernatant of adipose tissue-derived stem cells (ADSCs) significantly ameliorates fibrosis of different tissues, but limited attention has been paid to its efficacy on attenuating skin fibrosis. In this study, we explored the effect and possible mechanism of ADSC-conditioned medium (ADSC-CM) on HS.

Method
Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the expression of collagen I (Col1), collagen III (Col3), and α-smooth muscle actin (α-SMA) after fibroblasts and cultured HS tissues were stimulated with ADSC-CM and p38 inhibitor/activator. Immunofluorescence staining was performed to test the expression of α-SMA. Masson’s trichrome staining, hematoxylin and eosin (H&E) staining, and immunohistochemistry staining were carried out to assess the histological and pathological change of collagen in the BALB/c mouse excisional model. All data were analyzed by using SPSS17.0 software. Statistical analysis was performed by Student’s t tests.

Results
The in vitro and ex vivo study revealed ADSC-CM decreased the expression of Col1, Col3, and α-SMA. Together, thinner and orderly arranged collagen was manifested in HS tissues cultured with ADSC-CM. Dramatically, the assessed morphology showed an accelerated healing rate, less collagen deposition, and col1- and col3-positive cells in the ADSC-CM treated group. Importantly, the protein level of p-p38 was downregulated in a concentration-dependent manner in HS-derived fibroblasts with ADSC-CM treatment, which further decreased the expression of p-p38 after the application of its inhibitor, SB203580. SB203580 led to an obvious decline in the expression of Col1, Col3, and α-SMA in fibroblasts and cultured HS tissues and presented more ordered arrangement and thinner collagen fibers in BALB/c mice. Lastly, anisomycin, an agonist of p38, upregulated the expression of fibrotic proteins and revealed more disordered structure and denser collagen fibers.

Conclusion
This study demonstrated that ADSC-CM could decrease collagen deposition and scar formation in in vitro, ex vivo and in vivo experiments. The regulation of the p38/MAPK signaling pathway played an important role in the process. The application of ADSC-CM may provide a novel therapeutic strategy for HS treatment, and the anti-scarring effect can be achieved by inhibition of the p38/MAPK signaling pathway.
Therapeutic effects of human gingiva-derived mesenchymal stromal cells on murine contact hypersensitivity via prostaglandin E2–EP3 signaling
Authors: Li Pei , Zhao Yuming , Ge Lihong 
Published: August 2016

Abstract
Background
The immunomodulatory and anti-inflammatory functions of human gingiva-derived mesenchymal stromal cells (GMSCs) have been demonstrated in contact hypersensitivity (CHS) models; however, their therapeutic effect during the late phase of CHS has been poor.

Methods
The murine CHS model was induced by applying oxazolone to the ears of mice. Mesenchymal stromal cells were applied via two methods (intravenous or local injection) at three time points: 1 day before sensitization, 1 day before challenge, or 1 h after challenge. Prostaglandin E2 (PGE2) and sulprostone were administered subcutaneously 1 h after challenge.

Results
The application of GMSCs, bone marrow mesenchymal stem cells, and adipose-derived stem cells all effectively suppressed CHS; however, GMSC treatment exhibited the greatest efficacy. Local injection of GMSCs led to a more marked attenuation of CHS compared with intravenous injection, especially during the late phase of CHS, and this manifested as decreased infiltration of inflammatory cells, suppression of the levels of various proinflammatory cytokines, reconstruction of the disrupted Th1/Th2 balance, and upregulation of regulatory T cells in the allergen contact areas. Pretreatment with indomethacin significantly abrogated the GMSC-mediated immunosuppressive effects, while PGE2 application reversed the effects of indomethacin pretreatment of GMSCs. Moreover, GMSC administration promoted the expression of EP3, a prostaglandin E receptor, and the application of sulprostone, an agonist of EP3, significantly attenuated CHS to a similar degree as that of GMSC administration.

Conclusions
GMSCs have reproducible and powerful immunomodulatory functions. Local injection of GMSCs is the superior mode for therapeutic application. PGE2–EP3 signaling plays an important role in the immunomodulatory functions of GMSCs in murine CHS.
Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages
Authors: Mansour Tamer A , Rosenthal Joshua J C , Brown C Titus , Roberson Loretta M 
Published: August 2016

Abstract

Background
Porites astreoides is a ubiquitous species of coral on modern Caribbean reefs that is resistant to increasing temperatures, overfishing, and other anthropogenic impacts that have threatened most other coral species. We assembled and annotated a transcriptome from this coral using Illumina sequences from three different developmental stages collected over several years: free-swimming larvae, newly settled larvae, and adults (&gt;10 cm in diameter). This resource will aid understanding of coral calcification, larval settlement, and host–symbiont interactions.

Findings
A de novo transcriptome for the P. astreoides holobiont (coral plus algal symbiont) was assembled using 594 Mbp of raw Illumina sequencing data generated from five age-specific cDNA libraries. The new transcriptome consists of 867 255 transcript elements with an average length of 685 bases. The isolated P. astreoides assembly consists of 129 718 transcript elements with an average length of 811 bases, and the isolated Symbiodinium sp. assembly had 186 177 transcript elements with an average length of 1105 bases.

Conclusions
This contribution to coral transcriptome data provides a valuable resource for researchers studying the ontogeny of gene expression patterns within both the coral and its dinoflagellate symbiont.
Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion
Authors: You Jungmok , Heo June Seok , Kim Hyun Ok , Kim Eunkyoung 
Published: August 2016

Abstract

Background
Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure.

Results
The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film.

Conclusion
We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.
The role of acroblast formation duringDrosophilaspermatogenesis
Authors: Fári Karolina , Takács Sándor , Ungár Dániel , Sinka Rita 
Published: August 2016

ABSTRACTProtein recycling is important for maintaining homeostasis of the Golgi and its cisternae. The Vps54 (Scat) protein, a subunit of the GARP tethering complex, is a central factor in retrograde transport to the trans-Golgi. We found the scat1 mutant to be male sterile in Drosophila with individualization problems occurring during spermatogenesis. Another typically observed phenotype was the abnormal nuclear structure in elongated mutant cysts. When examining the structure and function of the Golgi, a failure in acrosome formation and endosome-Golgi vesicular transport were found in the scat1 mutant. This acrosome formation defect was due to a fault in the trans-Golgi side of the acroblast ribbon. When testing a mutation in a second retrograde transport protein, Fws, a subunit of the conserved oligomeric Golgi (COG) tethering complex, the acroblast structure, was again disrupted. fwsP caused a similar, albeit milder, acrosome and sperm individualization phenotype as the scat1 mutant. In the case of fwsP the cis side of the acroblast ribbon was dispersed, in-line with the intra-Golgi retrograde function of COG. Our results highlight the importance of an intact acroblast for acrosome formation, nuclear elongation and therefore sperm maturation. Moreover, these results suggest the importance of retrograde tethering complexes in the formation of a functional Golgi ribbon.
Biomedical Mutation Analysis (BMA): A software tool for analyzing mutations associated with antiviral resistance
Authors: Salvatierra Karina , Florez Hector 
Published: August 2016

Introduction:Hepatitis C virus (HCV) is considered a major public health problem, with 200 million people infected worldwide. The treatment for HCV chronic infection with pegylated interferon alpha plus ribavirin inhibitors is unspecific; consequently, the treatment is effective in only 50% of patients infected. This has prompted the development of direct-acting antivirals (DAA) that target virus proteins. These DAA have demonstrated a potent effectin vitroandin vivo; however, virus mutations associated with the development of resistance have been described.Objective: To design and develop an online information system for detecting mutations in amino acids known to be implicated in resistance to DAA.Materials and methods: We have used computer applications, technological tools, standard languages, infrastructure systems and algorithms, to analyze positions associated with resistance to DAA for the NS3, NS5A, and NS5B genes of HCV.Results: We have designed and developed an online information system named Biomedical Mutation Analysis (BMA), which allows users to calculate changes in nucleotide and amino acid sequences for each selected sequence from conventional Sanger and cloning sequencing using a graphical interface.Conclusion: BMA quickly, easily and effectively analyzes mutations, including complete documentation and examples. Furthermore, the development of different visualization techniques allows proper interpretation and understanding of the results.The data obtained using BMA will be useful for the assessment and surveillance of HCV resistance to new antivirals, and for the treatment regimens by selecting those DAA to which the virus is not resistant, avoiding unnecessary treatment failures. The software is available at:http://bma.itiud.org.
Standardization of Process Formation of Accounting Information
Authors: Корабельникова  , Korabelnikova Lyudmila 
Published: August 2016

Th e article is devoted to the problem of improving the quality of accounting information by synthesizing the
theory of the formation of accounting information and quality management theory by determining the composition of
the internal standards of the economic entity in the context of the stages of registration process and their linking with the
generated qualitative characteristics of accounting information.
What Causes Care Coordination Problems? A Case for Microanalysis
Authors: Zachary Wayne , Maulitz Russell Charles , Zachary Drew A. 
Published: August 2016

Introduction: Care coordination (CC) is an important fulcrum for pursuing a range
of health care goals. Current research and policy analyses have focused on aggregated
data rather than on understanding what happens within individual cases. At the case
level, CC emerges as a complex network of communications among providers over time,
crossing and recrossing many organizational boundaries. Micro-level analysis is needed
to understand where and how CC fails, as well as to identify best practices and root
causes of problems.Coordination Process Diagramming: Coordination Process Diagramming
(CPD) is a new framework for representing and analyzing CC arcs at the micro level,
separating an arc into its participants and roles, communication structure,
organizational structures, and transitions of care, all on a common time
line.Conclusion: Comparative CPD analysis across a sample of CC arcs identifies common
CC problems and potential root causes, showing the potential value of the framework. The
analyses also suggest intervention strategies that could be applied to attack the root
causes of CC problems, including organizational changes, education and training, and
additional health information technology development.
Feedforward consequences of isometric contractions: effort and ventilation
Authors: Luu Billy L. , Smith Janette L. , Martin Peter G. , McBain Rachel A. , Taylor Janet L. , Butler Jane E. 
Published: August 2016

AbstractThe onset of voluntary muscle contractions causes rapid increases in ventilation and is accompanied by a sensation of effort. Both the ventilatory response and perception of effort are proportional to contraction intensity, but these behaviors have been generalized from contractions of a single muscle group. Our aim was to determine how these relationships are affected by simultaneous contractions of multiple muscle groups. We examined the ventilatory response and perceived effort of contraction during separate and simultaneous isometric contractions of the contralateral elbow flexors and of an ipsilateral elbow flexor and knee extensor. Subjects made 10‐sec contractions at 25, 50, and 100% of maximum during normocapnia and hypercapnia. For simultaneous contractions, both muscle groups were activated at the same intensities. Ventilation was measured continuously and subjects rated the effort required to produce each contraction. As expected, ventilation and perceived effort increased proportionally with contraction intensity during individual contractions. However, during simultaneous contractions, neither ventilation nor effort reflected the combined muscle output. Rather, the ventilatory response was similar to when contractions were performed separately, and effort ratings showed a small but significant increase for simultaneous contractions. Hypercapnia at rest doubled baseline ventilation, but did not affect the difference in perceived effort between separate and simultaneous contractions. The ventilatory response and the sense of effort at the onset of muscle activity are not related to the total output of the motor pathways, or the working muscles, but arise from cortical regions upstream from the motor cortex.
Biomedical and Health Informatics Education – the IMIA Years
Authors: Mantas J. 
Published: August 2016

Summary
Objective: This paper presents the development of medical informatics education during the years from the establishment of the International Medical Informatics Association (IMIA) until today.
Method: A search in the literature was performed using search engines and appropriate keywords as well as a manual selection of papers. The search covered English language papers and was limited to search on papers title and abstract only.
Results: The aggregated papers were analyzed on the basis of the subject area, origin, time span, and curriculum development, and conclusions were drawn.
Conclusions: From the results, it is evident that IMIA has played a major role in comparing and integrating the Biomedical and Health Informatics educational efforts across the different levels of education and the regional distribution of educators and institutions. A large selection of references is presented facilitating future work on the field of education in biomedical and health informatics.
MULTILINGUAL INTERACTIONS IN A COLLABORATIVE SITUATION: THE EXAMPLE OF HIGH SCHOOL STUDENTS IN ST. MARTIN
Authors: CANDAU Olivier-Serge 
Published: August 2016

<p><strong>RÉSUMÉ.</strong><strong> </strong>Cet article vise à rendre compte de pratiques bilingues (anglais et français) d’élèves saint-martinois en situation collaborative afin d’enrichir le débat sur les langues et les savoirs. Cette recherche s’appuie sur deux corpus constitués d’interactions entre lycéens lors de séances de soutien scolaire, et étudiés selon l’analyse conversationnelle. Croisant modalité collaborative et didactique du bilinguisme, l’étude modélise la gestion des ressources langagières au regard des savoirs et des savoir-faire mobilisés durant l’interaction. Elle vise ainsi à établir en quoi, à quelles conditions et par quels mécanismes les interactions entre les deux langues interviennent dans la construction de compétences cognitives scolaires.</p><p><strong> </strong></p><p><strong>Mots-clés:</strong> <em>alternances de langues, compétences, collaboration, didactique du bilinguisme. </em></p><p><em><br /></em></p><p><em>00lns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"&gt;</em></p><p><strong>ABSTRACT.</strong><strong> </strong>This article aims to explore the bilingual practices (English and French) used by students from St Martin in a collaborative situation, in order to enrich the debate on languages and knowledge. This research is based on two corpora consisting of interactions between high school pupils during their educational support sessions, and which are studied using conversational analysis; the study uses this combination of collaborative methods and bilingual education to model the management of linguistic resources with regard to the factual knowledge and practical skills brought into play during the interaction. The challenge of this study is therefore to establish what is involved, and under which conditions and through which mechanisms the interactions between two languages (English and French) may contribute to the construction of cognitive learning skills.</p><p><strong> </strong></p><p><strong>Keywords: </strong><em>bilinguism</em><em> and teaching methods, code-switching, collaborative learning, learning skills.</em></p><p><em><br /></em></p>
“Quanto Mais Você Me Nega, Mais Eu Me Reafirmo”: Visibilidade e Afetos na Cena Negra Periférica Paulistana
Authors: Moutinho Laura , Alves Valéria , Mateuzi Milena 
Published: August 2016

Este texto tem por objetivo colocar em perspectiva alguns dosdebates políticos recentes que poderiam ser compreendidoscomo parte do que nomeamos como “marcadores sociais da diferença” e “interseccionalidade”. Tendo sido escrito por pesquisadorasqueocupamdiferentesposiçõesnavidasocial,napolítica e na esfera acadêmica, o objetivo mais amplo é o de cruzarexperiências e trajetórias no sentido de produzir uma reflexãoafinada com algumas ações políticas recentes sobre gênero eraça, feminismo e racismo/antirracismo e, por fim, sobre a desigualdade social.O cenário desta análise é a cidade de SãoPaulo, mas as experiências narradas e a análise dialogam com movimentos e iniciativas similares que vêm ocorrendo em diferentes partes do Brasil.