bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictions and outcomes of atrial fibrillation in the patients with acute myocardial infarction
Authors: Vukmirović Mihailo , Bošković Aneta , Tomašević Vukmirović Irena , Vujadinovic Radoje , Fatić Nikola , Bukumirić Zoran , Vukmirović Filip 
Published: May 2017

Abstract
The large epidemiological studies demonstrated that atrial fibrillation is correlated with high mortality and adverse events in patients with acute myocardial infarction. The aim of this study was to determinate predictors of atrial fibrillation develop during the hospital period in patients with acute myocardial infarction as well as short- and long-term mortality depending on the atrial fibrillation presentation. The 600 patients with an acute myocardial infarction were included in the study and follow-up 84 months. Atrial fibrillation develops during the hospital period was registered in 48 patients (8%). After adjustment by logistic regression model the strongest predictor of atrial fibrillation develop during the hospital period was older age, particularly more than 70 years (odds ratio 2.37, CI 1.23-4.58, p=0.010), followed by increased of Body Mass Index (odds ratio 1.17, CI 1.04-1.33, p=0.012), enlarged diameter of left atrium (LA) (odds ratio 1,18, CI 1,03-1,33, p=0,015) presentation of mitral regurgitation (odds ratio 3.56, CI 1.25-10.32, p=0.018) and B-type natriuretic peptide (odds ratio 2.12, CI 1.24-3.33, p=0.048).Patients with atrial fibrillation develop during the hospital period had a higher mortality during the hospital course (10.4% vs. 5.6%) p=0.179. as well as follow-up period of 84 months than patients without it (64.6% vs. 39.1%) p=0.569, than patients without it, but without statistically significance. Patients with AF develop during the hospital period had higher mortality during the hospital course as well as follow up period of 84 months than patients without it, but without statistically significance.
Crystal structure of n-butyl-tris(dicyclo-hexylamido)hafnium(IV), C40H75HfN3
Authors: Adler Christian , Schmidtmann Marc , Beckhaus Rüdiger 
Published: May 2017

Abstract
C40H75HfN3, monoclinic, P21/n (no. 14), a = 10.7446(3) Å, b = 13.6547(4) Å, c = 26.5464(9) Å, β = 92.2375(15)°, V = 3891.8(2) Å3, Z = 4, R
gt(F) = 0.0195, wR
ref(F
2) = 0.0461, T = 100(2) K.
Controlled stepwise synthesis of a Cu-MOF: Transmetallation of a magnesium precursor to a three-dimensional framework with very high solvent content
Authors: Guo Qianqian , Englert Ulli 
Published: May 2017

Abstract
A copper metal-organic framework (MOF) was synthesized by transmetalation. The ditopic ligand 3-(3,5-dimethyl-pyrazol-4-yl)pentane-2,4-dione is introduced via its Mg precursor complex in which it is oxygen-coordinated. After transmetallation, the ligand acts as N,O,O′ linker between dinuclear, sulfato-bridged Cu(II) nodes. The resulting three-dimensional solid crystallizes in the chiral space group I213, with more than half of its cell volume occupied by disordered methanol and dichloromethane molecules: the coordination framework encloses a large solvent-filled region along [1 1 1] which coounts for 54.8% of the crystal volume. The structure collapses upon removal of the clathrated solvent. Sulfate-O atoms are exposed towards the solvent region and facilitate trapping of polar guest molecules. The framework topology corresponds to a lcv net with Vertex symbol 3·3·102·102·103·103.
Reconceptualización de la educación en la era digital: educominicacion, redes de aprendizaje y cerebro factores claves en los actuales escenarios de construcción de conocimiento
Authors: Rodríguez García Yeldy Milena 
Published: May 2017

El concepto de educación requiere una reconceptualización, por parte de docentes y estudiantes, de cara a los desafíos de la revolución digital en términos comunicativos, que impactan directamente en los procesos educativos. Alta concordancia entre la conceptualización y la realidad educativa actual, reduce las posibilidades de sufrir frustración, disminuir la motivación, por ende el aprendizaje y no cumplir las expectativas. El artículo presenta resultados parciales de tesis doctoral denominada: “Reconceptualización de la educación en la era digital. Educomunicación, redes de aprendizaje y cerebro: una visión desde la neurociencia cognitiva a los procesos de construcción de conocimiento en entornos digitales” realizada en el contexto universitario colombiano (años 2012 a 2016). El fundamento teórico es la filosofía y la práctica educomunicativa y el enfoque Conectivista. El objeto principal fue analizar los conceptos de educación digital, las practicas educomunicativas, de redes de aprendizaje y perfiles cognitivos de muestra de 3643 estudiantes y 300 docentes de diferentes programas a nivel de pregrado, ambos neófitos en educación virtual y evidenciar si existía alguna relación con los procesos de construcción de conocimiento. Los resultados sugieren, que los preconceptos sobre las prácticas educativas digitales de docentes y estudiantes, influyen de forma significativa en los procesos de aprendizaje, comunicación, conductas y convivencia en estos entornos, pero también las evidencias cuantitativas y cualitativas soportan que estos preconceptos cambian en sentido positivo, cuando se experimentan didácticas acordes a la edad, al estilo cognitivo y con un enfoque educomunicativo y relacional. Las conclusiones presentan aspectos por mejorar y prospectiva.
Comparative Transcriptomic Analysis of Race 1 and Race 4 of Fusarium oxysporum f. sp. cubense Induced with Different Carbon Sources
Authors: Qin Shiwen , Ji Chunyan , Li Yunfeng , Wang Zhenzhong 
Published: May 2017

Abstract
The fungal pathogen Fusarium oxysporum f. sp. cubense causes Fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. Pathogenic race 1 attacks the “Gros Michel” banana cultivar, and race 4 is pathogenic to the Cavendish banana cultivar and those cultivars that are susceptible to Foc1. To understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed RNA sequencing to compare the genome-wide transcriptional profiles of the two races grown in media containing banana cell wall, pectin, or glucose as the sole carbon source. Overall, the gene expression profiles of Foc1 and Foc4 in response to host cell wall or pectin appeared remarkably different. When grown with host cell wall, a much larger number of genes showed altered levels of expression in Foc4 in comparison with Foc1, including genes encoding carbohydrate-active enzymes (CAZymes) and other virulence-related genes. Additionally, the levels of gene expression were higher in Foc4 than in Foc1 when grown with host cell wall or pectin. Furthermore, a great majority of genes were differentially expressed in a variety-specific manner when induced by host cell wall or pectin. More specific CAZymes and other pathogenesis-related genes were expressed in Foc4 than in Foc1 when grown with host cell wall. The first transcriptome profiles obtained for Foc during degradation of the host cell wall may provide new insights into the mechanism of banana cell wall polysaccharide decomposition and the genetic basis of Foc host specificity.
Novel Mutations in Synaptic Transmission Genes Suppress Neuronal Hyperexcitation inCaenorhabditis elegans
Authors: McCulloch Katherine A , Qi Yingchuan B , Takayanagi-Kiya Seika , Jin Yishi , Cherra Salvatore J 
Published: May 2017

AbstractAcetylcholine (ACh) receptors (AChR) regulate neural circuit activity in multiple contexts. In humans, mutations in ionotropic acetylcholine receptor (iAChR) genes can cause neurological disorders, including myasthenia gravis and epilepsy. In Caenorhabditis elegans, iAChRs play multiple roles in the locomotor circuit. The cholinergic motor neurons express an ACR-2-containing pentameric AChR (ACR-2R) comprised of ACR-2, ACR-3, ACR-12, UNC-38, and UNC-63 subunits. A gain-of-function mutation in the non-α subunit gene acr-2 [acr-2(gf)] causes defective locomotion as well as spontaneous convulsions. Previous studies of genetic suppressors of acr-2(gf) have provided insights into ACR-2R composition and assembly. Here, to further understand how the ACR-2R regulates neuronal activity, we expanded the suppressor screen for acr-2(gf)-induced convulsions. The majority of these suppressor mutations affect genes that play critical roles in synaptic transmission, including two novel mutations in the vesicular ACh transporter unc-17. In addition, we identified a role for a conserved major facilitator superfamily domain (MFSD) protein, mfsd-6, in regulating neural circuit activity. We further defined a role for the sphingosine (SPH) kinase (Sphk) sphk-1 in cholinergic neuron activity, independent of previously known signaling pathways. Overall, the genes identified in our study suggest that optimal modulation of synaptic activity is balanced by the differential activities of multiple pathways, and the novel alleles provide valuable reagents to further dissect neuronal mechanisms regulating the locomotor circuit.
Pervasive Behavioral Effects of MicroRNA Regulation inDrosophila
Authors: Picao-Osorio Joao , Lago-Baldaia Ines , Patraquim Pedro , Alonso Claudio R 
Published: May 2017

AbstractPicao-Osorio et al. reveal pervasive effects of microRNA regulation on complex locomotor behaviors in Drosophila larvae: over 40% of microRNAs display...The effects of microRNA (miRNA) regulation on the genetic programs underlying behavior remain largely unexplored. Despite this, recent work in Drosophila shows that mutation of a single miRNA locus (miR-iab4/iab8) affects the capacity of the larva to correct its orientation if turned upside down (self-righting, SR), suggesting that other miRNAs might also be involved in behavioral control. Here we explore this possibility, studying early larval SR behavior in a collection of 81 Drosophila miRNA mutants covering almost the entire miRNA complement of the late embryo. Unexpectedly, we observe that &gt;40% of all miRNAs tested significantly affect SR time, revealing pervasive behavioral effects of miRNA regulation in the early larva. Detailed analyses of those miRNAs affecting SR behavior (SR-miRNAs) show that individual miRNAs can affect movement in different ways, suggesting that specific molecular and cellular elements are affected by individual miRNA mutations. Furthermore, gene expression analysis shows that the Hox gene Abdominal-B (Abd-B) represents one of the targets deregulated by several SR-miRNAs. Our work thus reveals pervasive effects of miRNA regulation on a complex innate behavior in Drosophila and suggests that miRNAs may be core components of the genetic programs underlying behavioral control in other animals too.
GSK-3β Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2
Authors: Demin Annie Albert , Lee Miju , Lee Chul-Hwan , Seo Yeon-Soo 
Published: May 2017

Abstract
Lagging strand synthesis is mechanistically far more complicated than leading strand synthesis because it involves multistep processes and requires considerably more enzymes and protein factors. Due to this complexity, multiple fail-safe factors are required to ensure successful replication of the lagging strand DNA. We attempted to identify novel factors that are required in the absence of the helicase activity of Dna2, an essential enzyme in Okazaki-fragment maturation. In this article, we identified Rim11, a GSK-3β-kinase homolog, as a multicopy suppressor of dna2 helicase-dead mutant (dna2-K1080E). Subsequent epistasis analysis revealed that Ume6 (a DNA binding protein, a downstream substrate of Rim11) also acted as a multicopy suppressor of the dna2 allele. We found that the interaction of Ume6 with the conserved histone deacetylase complex Sin3-Rpd3 and the catalytic activity of Rpd3 were indispensable for the observed suppression of the dna2 mutant. Moreover, multicopy suppression by Rim11/Ume6 requires the presence of sister-chromatid recombination mediated by Rad52/Rad59 proteins, but not vice versa. Interestingly, the overexpression of Rim11 or Ume6 also suppressed the MMS sensitivity of rad59Δ. We also showed that the lethality of dna2 helicase-dead mutant was attributed to checkpoint activation and that decreased levels of deoxynucleotide triphosphates (dNTPs) by overexpressing Sml1 (an inhibitor of ribonucleotide reductase) rescued the dna2 mutant. We also present evidence that indicates Rim11/Ume6 works independently but in parallel with that of checkpoint inhibition, dNTP regulation, and sister-chromatid recombination. In conclusion, our results establish Rim11, Ume6, the histone deacetylase complex Sin3-Rpd3 and Sml1 as new factors important in the events of faulty lagging strand synthesis.
Whole-Genome Restriction Mapping by “Subhaploid”-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding
Authors: Dou Jinzhuang , Dou Huaiqian , Mu Chuang , Zhang Lingling , Li Yangping , Wang Jia , Li Tianqi , Li Yuli , Hu Xiaoli , Wang Shi , Bao Zhenmin 
Published: May 2017

Abstract
Assembly of complex genomes using short reads remains a major challenge, which usually yields highly fragmented assemblies. Generation of ultradense linkage maps is promising for anchoring such assemblies, but traditional linkage mapping methods are hindered by the infrequency and unevenness of meiotic recombination that limit attainable map resolution. Here we develop a sequencing-based “in vitro” linkage mapping approach (called RadMap), where chromosome breakage and segregation are realized by generating hundreds of “subhaploid” fosmid/bacterial-artificial-chromosome clone pools, and by restriction site-associated DNA sequencing of these clone pools to produce an ultradense whole-genome restriction map to facilitate genome scaffolding. A bootstrap-based minimum spanning tree algorithm is developed for grouping and ordering of genome-wide markers and is implemented in a user-friendly, integrated software package (AMMO). We perform extensive analyses to validate the power and accuracy of our approach in the model plant Arabidopsis thaliana and human. We also demonstrate the utility of RadMap for enhancing the contiguity of a variety of whole-genome shotgun assemblies generated using either short Illumina reads (300 bp) or long PacBio reads (6–14 kb), with up to 15-fold improvement of N50 (∼816 kb-3.7 Mb) and high scaffolding accuracy (98.1–98.5%). RadMap outperforms BioNano and Hi-C when input assembly is highly fragmented (contig N50 = 54 kb). RadMap can capture wide-range contiguity information and provide an efficient and flexible tool for high-resolution physical mapping and scaffolding of highly fragmented assemblies.
Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae
Authors: Hodges Amelia J , Gloss Lisa M , Wyrick John J 
Published: May 2017

Abstract
The essential histone chaperone FACT plays a critical role in DNA replication, repair, and transcription, primarily by binding to histone H2A-H2B dimers and regulating their assembly into nucleosomes. While FACT histone chaperone activity has been extensively studied, the exact nature of the H2A and H2B residues important for FACT binding remains controversial. In this study, we characterized the functions of residues in the histone H2A and H2B acidic patch, which is important for binding many chromatin-associated factors. We found that mutations in essential acidic patch residues cause a defect in histone occupancy in yeast, even though most of these histone mutants are expressed normally in yeast and form stable nucleosomes in vitro. Instead, we show that two acidic patch residues, H2B L109 and H2A E57, are important for histone binding to FACT in vivo. We systematically screened mutants in other H2A and H2B residues previously suspected to be important for FACT binding and confirmed the importance of H2B M62 using an in-vivo FACT-binding assay. Furthermore, we show that, like deletion mutants in FACT subunits, an H2A E57 and H2B M62 double mutant is lethal in yeast. In summary, we show that residues in the nucleosome acidic patch promote histone occupancy and are important for FACT binding to H2A-H2B dimers in yeast.
Legal Research Methodology and the Dream of Interdisciplinarity
Authors: Kroeze Irma J 
Published: May 2017

There are increasing calls for academics to abandon "traditional" disciplinary research and to engage in multi-, inter- and transdisciplinary research. The argument is that this will serve to break down working in "silos" and somehow lead to more innovative research. This article examines the concepts of multidisciplinary, interdisciplinary and transdisciplinary research to determine if this kind of research is possible in legal research. The basic premise is that science is unified by the need for some kind of justification, arguably in the form of falsifiability of theories. But science is also divided into natural, social and human sciences and this article argues that this division is based on methodological differences. Whilst the natural sciences employ a mostly empiricist methodology and the human sciences employ a mostly rationalist methodology, the social sciences seem to employ a mixture of the two methodologies. Law is a human science and moreover a professional discipline. Some argue that this professional nature militates against multi-, inter- and transdisciplinary (MIT) research as it requires law students to be taught how to "think like a lawyer". The article concludes that most law researchers engage in multidisciplinary research on a regular basis, but that interdisciplinary research is highly unlikely and transdisciplinary research almost never happens.
The Sexual Orientation of a Parent as a Factor when Considering Care
Authors: Feldhaus Chantelle 
Published: May 2017

Section 28(2) of the Constitution states that a child's best interest is of paramount importance in every matter concerning the child. Section 9 further provides that every person is considered equal before the law and has the right to equal protection and benefit of the law. Several grounds are listed relating to the unfair discrimination of persons, including their sexual orientation. The concept of care is incorporated in the Children's Act, and it entails a comprehensive description of parents' daily life regarding children and the powers and duties expected to ensure the general protection, well-being and best interests of the child. The aim of this contribution is to discuss the sexual orientation of a parent as a factor when considering care and the extent to which courts may give consideration to such a factor. The article will also address the question of whether or not the role of a parent's sexual orientation in determining the best interests of the child has changed since the common law concept of custody was replaced by the concept of care in the Children's Act. In this article, care and the best interests of the child will be discussed first. International law will be considered thereafter, followed by a discussion on the approach of our courts, pre- and post-1994, in order to come to a conclusion and make recommendations.
La práctica formativa como escenario de aprendizaje significativo del cuidado de enfermería
Authors: Flórez-Gutiérrez Carolina , Mayorga-Palacios Andrea Del Pilar , Vargas-Escobar Lina María 
Published: May 2017

<p>Los docentes en enfermería tienen la responsabilidad de formar profesionales en enfermería humanos, idóneos y competentes para cubrir las necesidades de cuidado de la población y afrontar los retos existentes en materia de salud. Las prácticas formativas representan un espacio enriquecedor para el aprendizaje del cuidado de manera significativa en el logro de este propósito. Este artículo presenta una reflexión en torno al aprendizaje del cuidado durante las prácticas formativas en un programa de enfermería de Bogotá. Se abordan tópicos como la importancia de la formación en el cuidado, la influencia del contexto institucional y del modelo pedagógico, así como los significados de la práctica para estudiantes y docentes. Se concluye que la apertura, el diálogo abierto, la motivación, el conocer el ser humano que hay en el estudiante, el promover su autonomía y su reflexión, y el acompañarlo en su experiencia como cuidador son aspectos esenciales para el aprendizaje significativo del cuidado de enfermería durante las prácticas formativas y que estos espacios se constituyen en experiencias transformadoras no solo para el estudiante sino también para el docente.</p><p> </p>
Evaluación del estado actual de la calidad hídrica y la percepción de la comunidad en el área de influencia de dos quebradas que nacen en el Páramo Rabanal, en los Municipios de Villapinzón (Cundinamarca) y Ventaquemada (Boyacá)
Authors: Guerrero Pedraza María Alejandra , Herrera Mejía Melissa Eliana 
Published: May 2017

<p>Este proyecto busca evaluar el estado actual de las condiciones del recurso hídrico de las Quebradas La Sierra (Villapinzón) y Yerbabuena (Ventaquemada), además de la percepción sobre la calidad hídrica de la comunidad en el área de influencia. En la metodología se realizaron visitas técnicas para la toma de muestras físico-químicas, biológicas y bacteriológicas del agua, y levantamiento de información a través de entrevistas a profundidad y análisis cartográfico; por medio de un enfoque mixto encaminado a estudiar las relaciones en el páramo. Según los resultados obtenidos la calidad biológica se obtuvo baja abundancia y riqueza de familias, mientras que en el análisis fisicoquímico la calificación ICA refleja una calidad buena (parámetros fuera del rango: coliformes fecales y pH) pero se evidencia alta intervención humana por el cambio del uso del suelo y así mismo se observó que la percepción acerca de los procesos ecológicos en la zona es escasa.</p><p>Abstract</p><p>Este proyecto busca evaluar el estado actual de las condiciones del recurso hídrico de las Quebradas La Sierra (Villapinzón) y Yerbabuena (Ventaquemada), además de la percepción sobre la calidad hídrica de la comunidad en el área de influencia. En la metodología se realizaron visitas técnicas para la toma de muestras físico-químicas, biológicas y bacteriológicas del agua, y levantamiento de información a través de entrevistas a profundidad y análisis cartográfico; por medio de un enfoque mixto encaminado a estudiar las relaciones en el páramo. Según los resultados obtenidos la calidad biológica se obtuvo baja abundancia y riqueza de familias, mientras que en el análisis fisicoquímico la calificación ICA refleja una calidad buena (parámetros fuera del rango: coliformes fecales y pH) pero se evidencia alta intervención humana por el cambio del uso del suelo y así mismo se observó que la percepción acerca de los procesos ecológicos en la zona es escasa.</p>
The impact of keyword and full video captioning on listening comprehension
Authors: Bensalem Elias Adam 
Published: May 2017

This study investigates the effect of two types of captioning (full and keyword captioning) on listening comprehension.Â Thirty-six university-level EFL students participated in the study. They were randomly assigned to watch three video clips under three conditions.Â The first group watched the video clips with full captions.Â The second group watched the same video clips with keyword captions. The control group watched the video clips without captions. After watching each clip, participants took a listening comprehension test. At the end of the experiment, participants completed a questionnaire to measure their perceptions about the use of captions and the video clips they watched. Results indicated that the full captioning group significantly outperformed both the keyword captioning and the no captioning group on the listening comprehension tests.Â However, this study did not find any significant difference between the keyword captioning group and the no captioning group. Results of the survey suggest that keyword captioning were a source of distraction for participants.Keywords: captions, video, listening comprehension, ESL.Â Â