bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies
Authors: Tomza‐Marciniak Agnieszka , Stępkowska Paulina , Kuba Jarosław , Pilarczyk Bogumiła 
Published: June 2017

AbstractAs bisphenol A (BPA) is characterized by a pronounced influence on human hormonal regulation, particular attention has been aimed at understanding its role in reproductive processes in males and females, as well as on fetal development. Owing to the increasing number of alarming reports on the negative consequences of the presence of BPA in human surroundings, more and more studies are being undertaken to clarify the negative effects of BPA on human reproductive processes. The aim of this work was to collect and summarize data on the influence of BPA exposure on reproductive health. Based on an analysis of selected publications it was stated that there is strong proof confirming that BPA is an ovarian, uterine and prostate toxicant at a level below the lowest observed adverse effect level (50 mg kg−1 bodyweight) as well as a level below the proposed safe level (4 μg kg−1 bodyweight). It seems there is also reliable evidence in relation to the negative effect of BPA on sperm quality and motility. Limited evidence also pertains to the case of the potential of BPA to affect polycystic ovary syndrome occurrence. Although in epidemiological studies this disease was common, in studies on animal models such results were still not confirmed. No unambiguous results of epidemiological studies and with animal models were obtained in relation to the evaluation of associations between BPA and implantation failure in women, evaluation of associations between BPA and sexual dysfunction in men, and impact of BPA on birth rate, birth weight and length of gestation. Copyright © 2017 John Wiley & Sons, Ltd.
Zearalenone induces ROS‐mediated mitochondrial damage in porcine IPEC‐J2 cells
Authors: Fan Wentao , Shen Tongtong , Ding Qiaoqi , Lv Yanan , Li Li , Huang Kehe , Yan Liping , Song Suquan 
Published: June 2017

AbstractIn this study, the mitochondrial damage effect and mechanism of zearalenone (ZEA) in swine small intestine IPEC‐J2 cells in vitro were comprehensively characterized. The analyses revealed that ZEA at high doses (8 and 7 μg/mL) can significantly increase P < 0.05 the malondialdehyde levels and decrease antioxidant enzymes activities after 48 h of exposure. Meanwhile, the reactive oxygen species (ROS) accumulation increased in high dose ZEA‐treated groups after 2 h treatment, but decreased due to the ROS‐induced mitochondrial damage and the caused cell apoptosis after 48 h of high does ZEA treatment. Moreover, the decreasing of mitochondrial membrane potential (MMP; ΔΨ) in high dose ZEA exposure was observed in line with the increasing ROS production in mitochondria. Results suggest that ZEA exposure can induce mitochondrial damage by reducing antioxidant enzyme activities, accumulation of ROS, and decreasing MMP. The mitochondrial damage had a dramatic concentration–effects relationship with ZEA.
Overexpression of Circular RNA ciRS‐7 Abrogates the Tumor Suppressive Effect of miR‐7 on Gastric Cancer via PTEN/PI3K/AKT Signaling Pathway
Authors: Pan Haiyan , Li Tao , Jiang Yugang , Pan Congcong , Ding Yuanlin , Huang Zhigang , Yu Haibing , Kong Danli 
Published: June 2017

ABSTRACTGastric cancer (GC) has one of the highest mortality rates of malignancies globally. Currently, ciRS‐7, a novel circular RNA, has emerged as a potential sponge for miR‐7. However, few studies on ciRS‐7 in GC have been performed. In this study, we investigated the clinical significance and function of ciRS‐7 in GC. First, the expression levels of ciRS‐7 in 102 primary GC tissues and the matched para‐carcinoma tissues were evaluated and the clinical relevance was confirmed in an independent validation cohort (n = 154). Second, the effects of ciRS‐7 on miR‐7, PTEN, and PI3K were evaluated. Finally, the function of ciRS‐7 in GC was analyzed with cell lines and nude mice. The expression of ciRS‐7 was significantly upregulated in GC tissues compared with the matched para‐carcinoma tissues (P = 0.0023), and the upregulation of ciRS‐7 was linked to poor survival in the testing (P = 0.0143) and validation cohort (P = 0.0061). Multivariate survival analysis revealed that ciRS‐7 was probably an independent risk factor of overall survival (P < 0.05). Furthermore, overexpression of ciRS‐7 blocked the miR‐7‐induced tumor suppression in MGC‐803 and HGC‐27 cells and led to a more aggressive oncogenic phenotype, via antagonizing miR‐7‐mediated PTEN/PI3K/AKT pathway. ciRS‐7 may act as a prospective prognostic biological marker and a promising therapeutic target for GC. J. Cell. Biochem. 119: 440–446, 2018. © 2017 Wiley Periodicals, Inc.
The Macrophage Activation Induced by Bacillus thuringiensis Cry1Ac Protoxin Involves ERK1/2 and p38 Pathways and the Interaction with Cell‐Surface‐HSP70
Authors: Rubio‐Infante Nestor , Ilhuicatzi‐Alvarado Damaris , Torres‐Martínez Marilu , Reyes‐Grajeda Juan Pablo , Nava‐Acosta Raúl , González‐González Edith , Moreno‐Fierros Leticia 
Published: June 2017

ABSTRACTHere, we aimed to further characterize the mechanisms involved in protoxin (p) Cry1Ac‐induced macrophage activation. We demonstrated that pCry1Ac induces MAPK ERK1/2, p38, and JNK phosphorylation in RAW264.7 macrophages. Because MAPK activation is mainly triggered via ligand–receptor interactions, we focused on the identification of potential pCry1Ac‐receptor proteins. Flow cytometry and confocal analysis showed specific saturable pCry1Ac‐binding to the macrophage surface and evidenced its internalization via the clathrin‐pathway. We performed immunoprecipitation assays and identified by MALDI‐TOF‐TOF several possible pCry1Ac‐binding proteins, such as heat shock proteins (HSPs), vimentin, α‐enolase, and actin; whose interaction and presence was confirmed, respectively, by ligand blot and Western blot assays. We also detected cell‐surface (cs) pCry1Ac‐HSP70 colocalization, so HSP70 was chosen for further characterization. Co‐immunoprecipitation with HSP70 antibodies followed by Western blot confirmed the pCry1Ac‐HSP70 interaction. Furthermore, pretreatment of RAW264.7 cells with HSP70 antibodies reduced pCry1Ac‐induced ERK1 phosphorylation and MCP‐1 production; thus suggest the functional participation of csHSP70 in pCry1Ac‐induced macrophage activation. csHSP70 also was evaluated in peritoneal‐cavity (PerC) macrophages of untreated BALB/c mice, interestingly it was found that the predominant population namely large‐peritoneal‐macrophages (LPM) displayed csHSP70 + hi. Furthermore, the dynamics of PerC macrophage subsets, LPM, and small‐peritoneal macrophages (SPM) were evaluated in response to in vivo pCry1Ac stimuli in presence or not of phenylethynesulfonamide (PES) a functional HSP70 inhibitor. It was found that pCry1Ac increased the proportion of SPM CD11b + F4/80 + lowMHCII + csHSP70 + low and markedly reduced the amount of LPM CD11b + F4/80 + hiMHCII‐csHSP70 + hi; while PES, partially suppressed this pCry1Ac‐induced effect, further suggesting the participation of HSP70 in macrophage activation process. J. Cell. Biochem. 119: 580–598, 2018. © 2017 Wiley Periodicals, Inc.
Multiple Functions of Long Non‐Coding RNAs in Oxidative Stress, DNA Damage Response and Cancer Progression
Authors: Tehrani Sadra Samavarchi , Karimian Ansar , Parsian Hadi , Majidinia Maryam , Yousefi Bahman 
Published: June 2017

ABSTRACTIn addition to aberrant alternation of transcriptome, it is now suggested that dysregulation of the non‐coding transcripts, particularly long non‐coding RNAs (lncRNAs), which comprise the majority of the genome, is contributed to cancer initiation and progression. As the result of recent huge efforts, the possible roles of numerous lncRNAs in the human cancers were characterized, as well as various strategies with inhibitory effects to target these transcripts on the transformed cells. Moreover, DNA damage response (DDR) pathway is a complex regulatory network responsible for the identification of disruptions in DNA structure, integrity and stability‐ it is reported to be associated with the up‐regulation and down‐regulation of lncRNAs. This review explores the involvement of the various lncRNAs in different human cancers, afterwards discusses the association of the lncRNAs expression with the DDR and oxidative stress, which are implicated in a myriad pathophysiological and physiological intra‐ and extracellular damages. J. Cell. Biochem. 119: 223–236, 2018. © 2017 Wiley Periodicals, Inc.
Distinct Coping Profiles Are Associated With Mental Health Differences in Transgender and Gender Nonconforming Adults
Authors: Freese Rebecca , Ott Miles Q. , Rood Brian A. , Reisner Sari L. , Pantalone David W. 
Published: June 2017

ObjectiveThis study assessed the unique coping strategies of transgender and gender nonconforming (TGNC) individuals in the United States used to manage gender‐related stress, and examined associations between specific coping profiles and mental health.MethodsData were from 316 participants in the 2014–2015 Transgender Stress and Health Study, an online study of TGNC mental and sexual health. A factor analysis of the coping measure (Brief COPE) was followed by a k‐means cluster analysis to evaluate distinct profiles of coping with gender‐related stress. Proportional odds models and logistic regression models indicated how coping profiles related to levels of self‐reported depressive symptoms and suicidality.ResultsA 4‐factor structure was identified with three distinct profiles of coping with gender‐related stress, each representative of the frequency (high or low) in which participants used functional and dysfunctional coping strategies: (a) high‐functional/low‐dysfunctional, (b) high‐functional/high‐dysfunctional, and (c) low‐functional/low‐dysfunctional. There were significant differences in depressive symptoms and suicidality based on distinct gender‐related coping profiles. The high‐functional/high‐dysfunctional group reported significantly poorer mental health compared with the high‐functional/low‐dysfunctional group.ConclusionTo improve mental health outcomes in TGNC individuals, health providers and researchers should strive to not only promote functional coping strategies for managing gender‐related stress but also decrease dysfunctional coping strategies.
Sensitivity of temperature to physical parameterization schemes of RegCM4 over the CORDEX‐Southeast Asia region
Authors: Cruz F. T. , Narisma G. T. , Dado J. B. , Singhruck P. , Tangang F. , Linarka U. A. , Wati T. , Juneng L. , Phan‐Van T. , Ngo‐Duc T. , Santisirisomboon J. , Gunawan D. , Aldrian E. 
Published: June 2017

ABSTRACTThis study examines the simulated temperature over Southeast Asia (SEA) using the Regional Climate Model version 4.3 (RegCM4.3), and its sensitivity to selected cumulus and ocean surface flux schemes. Model simulations were conducted for the SEA domain at 36 km spatial resolution for the period of 1989–2008, as part of the Southeast Asia Regional Climate Downscaling/Coordinated Regional Climate Downscaling Experiment‐Southeast Asia (SEACLID/CORDEX‐Southeast Asia) project. A total of 18 sensitivity experiments were conducted with a combination of six cumulus parameterization schemes and three ocean surface flux schemes. The model's skill in representing mean, maximum and minimum temperatures is evaluated against observed gridded data sets. Results indicate a predominant cold bias in all simulations, particularly over mainland SEA (Indochina) during the season of December to February. Nevertheless, the seasonal correlation is highest over this region. The cold bias of the model is also evident in the temperature distributions, such that there are more cold months than observed, which may be associated with the underestimation of the daily maximum temperature. A few simulations also reveal a warm bias over some areas in the Maritime Continent. Further examination shows that both radiative and surface fluxes influence the simulated temperature, which may also have effects that partially offset each other in some areas. Comparison of the sensitivity experiments reveals differences in model performance, and underlines the importance in choosing the appropriate configuration for RegCM4.3 before it is used to downscale climate projections, particularly for the SEA region. This study also shows a strong influence of the choice of cumulus scheme on temperature. Based on performance metrics for temperature among the schemes tested, the Massachusetts Institute of Technology (MIT) Emanuel cumulus scheme and the Biosphere‐Atmosphere Transfer Scheme version 1e (BATS1e) ocean surface flux scheme can be used in future simulations for the region.
Seasonal variability and synoptic characteristics of dust cases over southwestern Saudi Arabia
Authors: Mashat Abdul‐Wahab S. , Alamoudi Ahmad O. , Awad Adel M. , Assiri Mazen E. 
Published: June 2017

ABSTRACTThe synoptic characteristics and statistical variability of seasonal dust over southwestern Saudi Arabia are studied for the period from 1979 to 2006 using the aerosol index (AI) from the Total Ozone Mapping Spectrometer (TOMS) satellite, dust observations from surface stations, and meteorological data from the National Center for Environmental Prediction and the National Center for Atmospheric Research (NCEP/NCAR) reanalysis data set.The seasonal AI distribution indicates that approximately 80% of each year was dusty and that most of the dust occurred during hot months. In addition, the surface observations of the dust types show that the stations with the largest number of dust observations throughout the year were close to the desert, except during the summer, when the stations near the Red Sea had the largest number of dust observations.The synoptic forces that influenced the dust cases were the relative positions of high‐pressure systems (Azores or Siberian) and low‐pressure systems (Sudan or Indian), alongside their interactions. The relative positions of the atmospheric systems are highly pronounced at a pressure level of 850 hPa; at this pressure, the systems are oriented from north (anticyclonic system) to south (cyclonic system), turn anticlockwise to become oriented from west (anticyclonic system) to east (cyclonic system) during the summer, and then turn clockwise during the winter. Moreover, the interaction of the atmospheric systems influences the wind pattern of the seasonal composition over the southern Arabian Peninsula, which produces an anticyclonic wind pattern during winter, a cyclonic wind pattern during spring, a northerly/northwesterly wind pattern during summer and an anticyclonic wind pattern during autumn.The dust sources changed because of the relative positions of these atmospheric systems, in which the ‘Toker Gap’ Sudan was the summer/autumn dust source, and the ‘central and eastern’ Arabian Peninsula was the winter/spring dust source.
Is axillary surgery beneficial for patients with adenoid cystic carcinoma of the breast?
Authors: Welsh Jessemae L. , Keeney Michael G. , Hoskin Tanya L. , Glazebrook Katrina N. , Boughey Judy C. , Shah Sejal S. , Hieken Tina J. 
Published: June 2017

Background and ObjectivesAdenoid cystic carcinoma (ACC) is a rare, typically triple‐negative, breast cancer reported to have a favorable prognosis and low rate of nodal metastasis. No consensus guidelines exist for axillary staging and treatment.MethodsWe identified all patients with ACC evaluated at our institution from January 1994 to August 2016. Patient, tumor, and treatment variables were abstracted and analyzed.ResultsWe identified 20 pure ACCs (0.13% of all invasive breast cancers) with size range 0.2‐4.8 cm, in 19 women, median age 59 years. Preoperative axillary ultrasound was normal in 10/13 women and suspicious in 3/13 who had a subsequent negative lymph node fine needle aspiration (FNA). Fifteen patients (75%) had sentinel lymph node surgery and were pathologically node‐negative, while the remaining five had no axillary surgery. With 3.6 years median follow‐up (range 0.2‐38.6 years), three patients experienced an in‐breast recurrence at 2, 16, and 17 years, respectively, while none recurred in regional nodes.ConclusionsWe observed no cases of nodal metastasis in 20 consecutive cases of ACC of the breast. Preoperative axillary ultrasound with FNA of suspicious nodes accurately predicted pathologic nodal stage. These data suggest axillary surgery might be omitted safely in patients with pure ACC and a clinically negative axilla.
Xeno‐pericardial patch repair of the inferior vena cava for radical resection of renal cell carcinoma with tumor thrombus
Authors: Fukushima Satsuki , Uemura Motohide , Gotoh Kunihito , Ujike Takeshi , Wada Hiroshi , Miyagawa Shigeru , Toda Koichi , Sawa Yoshiki 
Published: June 2017

Background and ObjectivesFor tumor thrombus in the inferior vena cava (IVC) complicated with kidney cancer, we built a surgical team to achieve (1) en bloc tumor resection; (2) xeno‐pericardial patch IVC repair; and (3) minimum organ damages. We reviewed outcome of the case series to verify rationale of this approach.MethodsA consecutive series of 12 patients having the IVC tumor thrombus by renal cell carcinoma in the last 3 years was enrolled. Minimum kidney ischemia was induced in five cases (Procedure I), whereas liver and kidney ischemia was induced in five cases (Procedure II). Mild hypothermic extracorporeal circulation was used in two cases (Procedure III).ResultsThere was no mortality or severe morbidities related to the surgery. Postoperative recovery was most prompt by the Procedure I. Liver and kidney ischemic time was longer in the Procedure III than the Procedure II, whereas organ function was not substantially impaired in either series. The resected IVC margin was free from the cancer in all cases, while local recurrence was not seen in any cases.ConclusionsEn bloc resection with xeno‐pericardial patch repair of the IVC was successfully performed in the tumor thrombus complicated with kidney cancer with minimum organ damage.
The natural history of recoverable vocal fold paralysis: Implications for kinetics of reinnervation
Authors: Mau Ted , Pan Hao‐Min , Childs Lesley F. 
Published: June 2017

Objectives/HypothesisPatients with unilateral vocal fold paralysis (UVFP) are commonly told to wait 12 months for spontaneous recovery. This study aims to 1) determine the time to vocal recovery in UVFP, 2) use that data to develop a neurophysiologically plausible model for recovery, and 3) use the model to generate meaningful predictions for patient counseling.Study DesignCase series with de novo mathematical modeling.MethodsPatients with UVFP who could pinpoint a discrete onset of vocal improvement were identified. The time‐to‐recovery data were modeled by assuming an “early” recovery group with neuropraxia and a “late” recovery group with more severe nerve injury. For the late group, a two‐stage model was developed to explain the time to recovery: regenerating axons must cross the site of injury in stage 1 (probabilistic), followed by unimpeded regrowth to the larynx in stage 2 (deterministic).ResultsOf 727 cases of UVFP over a 7‐year period, 44 reported spontaneous recovery with a discrete onset of vocal improvement. A hybrid distribution incorporating the two stages (exponentially modified Gaussian) accurately modeled the time‐to‐recovery data (R2 = 0.918). The model predicts 86% of patients with recoverable UVFP will recover within 6 months, with 96% recovering within 9 months. Earlier vocal recovery is associated with recovery of vocal fold motion and younger age.ConclusionsWaiting 12 months for spontaneous recovery is probably too conservative. Repair across the site of injury, and not regrowth to larynx, is likely the rate‐determining step in reinnervation, consistent with other works on peripheral nerve regeneration.Level of Evidence4. Laryngoscope, 127:2585–2590, 2017
Hot or Cold Ablation – That's the Question
Authors: Nießen Markus , Bürgermeister Lisa , Hambach Nelli 
Published: June 2017

AbstractA new generation of ultrafast lasers with high average powers and high repetition rates is coming to the market. Those systems in principle bring together high precision processing and high throughput. But with high average power thermal effects show up the demand for a careful process planning. A new simulation tool from Fraunhofer ILT incorporates such effects and allows for precise process optimization even at high average laser power.
Thermal Spraying with Diode Lasers
Authors: Molitor Thomas 
Published: June 2017

AbstractBoiler tubes in biomass and incineration plants are exposed to extreme operating conditions. To create vapor pressure within the pipes that makes an effective drive of the power‐plant turbines possible, flue gas temperatures of up to 1000 °C must be realized within the combustion chamber of the boiler. No pipe‐material will sustain the chemical processes induced in this way for very long. Already at temperatures of 350 °C, the chlorine in the flue gas increasingly reacts with the iron of the ferritic pipe materials. A rapid and progressive oxidation process begins and reduces the wall thickness of the pipes, which can lead – using unprotected pipes – to tube leakage in some cases within less than one year (Fig. 1). In order to improve the lifetime of pipes, many power plant operators count on metallic or ceramic corrosion‐protection coatings. With their help, the material decomposition process can be delayed and the lifetime of the pipes can be tripled or even quadrupled. However, the investment in a coating in most cases is profitable already if lifetime of pipes is doubled because the exchange of corroded pipes requires new tubes plus a temporary shutdown of the whole boiler system.
Paleofire Dynamics in Central Spain during the Late Holocene: The Role of Climatic and Anthropogenic Forcing
Authors: López‐Sáez José Antonio , Vargas Grettel , Ruiz‐Fernández Jesús , Blarquez Olivier , Alba‐Sánchez Francisca , Oliva Marc , Pérez‐Díaz Sebastián , Robles‐López Sandra , Abel‐Schaad Daniel 
Published: June 2017

ABSTRACTThe use of fire and, consequently, its severity and incidence on the environment have grown steadily during the last millennia throughout the Mediterranean. This issue can be assessed in several mountain ranges of central Iberia where changes in the management policy on anthropic activities and exploitation of high‐mountain environments have promoted a remarkable increase on fire frequency. Our research focuses on fire dynamics throughout the last 3,000 years from three peat bog charcoal records of the Gredos range (central Iberia). Our aim is to reconstruct past fire regimes according to forest vegetation typology (Castanea sativa, Pinus pinaster, and Pinus sylvestris). Charcoal influx shows low values between 3,140 and 1,800 cal. year bp when forests were relatively dense in both high and mid‐mountain areas. Fire appeared synchronous between 1,800 and 1,700 cal. year bp for Lanzahíta and Serranillos and around 1,400–1,240 cal. year bp for the three sites, suggesting anthropogenic fire control between the Late Roman and Visigothic periods that can be related to the cultivation of olive trees in the valleys and a greater human impact in high‐mountain areas. By contrast, during the Muslim period (1,240–850 cal. year bp), fire dynamics becomes asynchronous. Later, fires turn again coeval in the Gredos range during the Christian period (850–500 cal. year bp) and can be also correlated with drought phases during the Late Medieval Warm Episode. In short, our study demonstrates that fire activity has been enormously variable during the late Holocene in response to both short‐term and long‐term regional and global climate, vegetation dynamics, and land use changes. Copyright © 2017 John Wiley & Sons, Ltd.