bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automatic runtime calculation of communications for data‐parallel expressions with periodic conditions
Authors: Moreton‐Fernandez Ana , Gonzalez‐Escribano Arturo 
Published: January 2018

SummaryMany real‐world applications feature data accesses on periodic domains. Manually implementing the synchronizations and communications associated to the data dependences on each case is cumbersome and error‐prone. It is increasingly interesting to support these applications in high‐level parallel programming languages or parallelizing compilers. In this paper, we present a technique that, for distributed‐memory systems, calculates the specific communications derived from data‐parallel codes with or without periodic boundary conditions on affine access expressions. It makes transparent to the programmer the management of aggregated communications for the chosen data partition. Our technique moves to runtime part of the compile‐time analysis typically used to generate the communication code for affine expressions, introducing a complete new technique that also supports the periodic boundary conditions. We present an experimental study to evaluate our proposal using several study cases. Our experimental results show that our approach can automatically obtain communication codes as efficient as those found in MPI reference codes, reducing the development effort.
Differentiation and Contractile Analysis of GFP‐Sarcomere Reporter hiPSC‐Cardiomyocytes
Authors: Sharma Arun , Toepfer Christopher N. , Schmid Manuel , Garfinkel Amanda C. , Seidman Christine E. 
Published: January 2018

AbstractHuman induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CMs) represent a powerful cellular platform for illuminating mechanisms of human cardiovascular disease and for pharmacological screening. Recent advances in CRISPR/Cas9‐mediated genome editing technology underlie this profound utility. We have generated hiPSC‐CMs harboring fluorescently‐tagged sarcomeric proteins, which provide a tool to non‐invasively study human sarcomere function and dysfunction. In this unit, we illustrate methods for conducting high‐efficiency, small molecule‐mediated differentiation of hiPSCs into cardiomyocytes, and for performing non‐invasive contractile analysis through direct sarcomere tracking of GFP‐sarcomere reporter hiPSC‐CMs. We believe that this type of analysis can overcome sensitivity problems found in other forms of contractile assays involving hiPSC‐CMs by directly measuring contractility at the fundamental contractile unit of the hiPSC‐CM, the sarcomere. © 2018 by John Wiley & Sons, Inc.
An intelligent database proactive cache replacement policy for mobile communication system based on genetic programming
Authors: Darwish Saad M. , El‐Shnawy Amr G. 
Published: January 2018

SummaryIn the mobile environment, the movement of the users, disconnected modes, many data updates, power battery consumption, limited cache size, and limited bandwidth impose significant challenges in information access. Caching is considered one of the most important concepts to deal with these challenges. There are 2 general topics related to the client cache policy: cache invalidation method keeps data in the cache up to date and cache replacement method chooses the cached element(s) that would be removed from the cache once the cache stays full. The aim of this work is to introduce a new technique for cache replacement in a mobile database that takes into consideration the impact of invalidation time for enhancing data availability in the mobile environment by using genetic programming. In this case, each client collects information for every cached item in the cache like access probability, cached document size, and validation time and uses these factors in a fitness function to determine cached items that will be removed from the cache. The experiments were carried by NS2 simulator to assess the efficiency of the proposed method, and the outcomes are judged against existing cache replacement algorithms. It is concluded that the proposed approach performs significantly better than other approaches.
Selective extraction of high‐value phenolic compounds from distillation wastewater of basil (Ocimum basilicum L.) by pressurized liquid extraction
Authors: Pagano Imma , Sánchez‐Camargo Andrea del Pilar , Mendiola Jose Antonio , Campone Luca , Cifuentes Alejandro , Rastrelli Luca , Ibañez Elena 
Published: January 2018

AbstractDuring the essential oil steam distillation from aromatic herbs, huge amounts of distillation wastewaters (DWWs) are generated. These by‐products represent an exceptionally rich source of phenolic compounds such as rosmarinic acid (RA) and caffeic acid (CA). Herein, the alternative use of dried basil DWWs (dDWWs) to perform a selective extraction of RA and CA by pressurized liquid extraction (PLE) employing bio‐based solvent was studied. To select the most suitable solvent for PLE, the theoretical modelling of Hansen solubility parameters (HSP) was carried out. This approach allows reducing the list of candidate to two solvents: ethanol and ethyl lactate. Due to the composition of the sample, mixtures of water with those solvents were also tested. An enriched PLE extract in RA (23.90 ± 2.06 mg/g extract) with an extraction efficiency of 75.89 ± 16.03% employing a water‐ethanol mixture 25:75 (% v/v) at 50°C was obtained. In the case of CA, a PLE extract with 2.42 ± 0.04 mg/g extract, having an extraction efficiency of 13.86 ± 4.96% using ethanol absolute at 50°C was achieved. DWWs are proposed as new promising sources of natural additives and/or functional ingredients for cosmetic, nutraceutical, and food applications.
Effects of excessive fibrin deposit and polylactide adhesion barrier on wound healing in thyroidectomy murine wound model
Authors: Lim Yun‐Sung , Kim Hee‐Bok , Park Joo Hyun , Cho Chang Gun , Park Seok‐Won , Lee Jae Suk , Kim Eo Jin , Kwon Seong Keun 
Published: January 2018

AbstractBackgroundWe hypothesize that excessive fibrin formation and inflammation induced by antiadhesive material, SurgiWrap (SW), would have an adverse effect on wound healing. It was evaluated by a thyroidectomy murine wound model.MethodsExcessive fibrin formation was induced by isthmectomy without hemostasis. Rats were allocated into isthmectomy with SurgiWrap (I+SW+), I+SW‐, I‐SW+, I‐SW‐, and isthmectomy after electrocautery for hemostasis (I+C+SW‐). The SWs were placed on the superficial and visceral layers for gross and microscopic evaluation.ResultsMicroscopic examination showed collagen deposition occurred in the I‐SW‐ sham group and at a higher level in I+C+SW‐. The collagen deposition decreased in groups without SW with time but increased in groups with SW. Use of SW produced more inflammation and more collagen deposition. The I+SW + group developed the largest area of collagen deposition at 4 weeks and more collagen deposition than the I‐SW + group.ConclusionThe SW induced more collagen deposition increasing with time. The collagen deposition produced by SW was worsened by excessive fibrin formation and inflammation.
EVOLUTION IN THE GENUS RUELLIA (ACANTHACEAE): A DISCUSSION BASED ON FLORAL FLAVONOIDS
Authors: Bloom Miriam 
Published: January 2018

A clear dichotomy exists in the genus Ruellia, separating the blue from the red flowered species. Flavonoids differ in a qualitative rather than a quantitative way. Apigenin 7‐glucuronide and malvidin 3,5‐diglucoside are common to all the blue flowered species, whereas chalcononaringenin 2'‐glucoside (isosalipurposide) and pelargonidin 3,5‐diglucoside are shared by the red flowered ones.The blue flowered species are linked with the red via apigenin 7‐glucuronide and 3,5‐diglucosylation of their respective anthocyanins. Both groups are involved in flavonoid race formation. All examined species (and some populations within species) differ in flavonoid content. The patterns of variability displayed provide a basis upon which an evolutionary scheme is constructed. Genetic drift is hypothesized as the effector of race formation in the blue flowered group.
AFTER‐RIPENING AS A FACTOR IN LETTUCE SEED GERMINATION RESPONSE
Authors: Suzuki Yoshihiro 
Published: January 2018

The physiological state of lettuce seeds could be divided into three distinct stages. The first is the dormant stage, the second is the nondormant one and the third is the deteriorating one. The dormant and the deteriorating stages can be classified further into three phases, respectively, by the actions of germination temperature, red light, and chemical substances.The effects of an exposure to red light and of the application of gibberellic acid A3 and thiourea on germination can be divided into two functions: 1) dormancy‐releasing effect on dormant seeds, and 2) stimulating effect on aged seeds which have reduced their germinability.
SEXUAL REPRODUCTION AND MEIOSIS IN PERIDINIUM INCONSPICUUM LEMMERMANN (DINOPHYCEAE)
Authors: Pfiester Lois A. , Timpano P. , Skvarla J. J. , Holt J. R. 
Published: January 2018

InPeridinium inconspicuumLemmermann, sexual reproduction occurs in both nitrogen‐enriched and nitrogen‐deficient media. In this homothallic strain, protoplasmic fusion begins between two thecate gametes; but zygote formation is completed in a space outside the fusing pair. This diploid cell can form a plated theca which is shed as the cell enlarges. This spherical zygote then forms a new non‐plated theca. The process of ecdysis and the formation of a new non‐plated theca is repeated several times. During this process the zygote gradually elongates and by cytoplasmic infurrowing becomes peanut‐shaped. Eventually two cells are formed.The first and second meiotic divisions are greatly separated in time. The first meiotic division occurs in the spherical non‐thecate zygote. The second meiotic division can occur in the peanut‐shaped zygote before it completes cytokinesis. This meiotic division may not be synchronous, occasionally resulting in a trinucleate stage. Eventually four flagellated, haploid products are produced.
A cool response—The influence of ambient temperature on capillary refill time
Authors: John Rani Toll , Henricson Joakim , Junker Johan , Jonson Carl‐Oscar , Nilsson Gert E. , Wilhelms Daniel , Anderson Chris D. 
Published: January 2018

ObjectiveTo describe the effect of low ambient temperature on skin temperature and capillary refill (CR) time in forehead, sternum and finger pulp.MethodsAn observational, nonrandomized experimental study on 15 healthy subjects (6 females) in a cold room (8°C). Outcome measures were skin temperature and quantified CR test after application of a standardized blanching pressure (9 N/cm2) using digital photographic polarization spectroscopy to generate CR times.ResultsThe finger pulp showed marked temperature fall and prolonged CR times (>10 seconds). The CR registrations of the forehead and sternum were more comparable to curves observed in a control material at room temperature, and skin temperature falls were less marked. CR times were not prolonged in forehead measurements. At the sternum, some individuals showed CR times beyond guideline recommendations despite only a marginal reduction in skin temperature.ConclusionsLow ambient temperature is a strong independent factor for CR time at peripheral sites. Reservation about sternum as a site of measurement is warranted since cold provocation produced prolonged CR times in some individuals. We found that the forehead is the most thermostable of the 3 sites and thus the preferred site to avoid ambient temperature artifact in measuring CR time.
microRNA‐130a suppresses breast cancer cell migration and invasion by targeting FOSL1 and upregulating ZO‐1
Authors: Chen Xiaowei , Zhao Min , Huang Jin , Li Yuhuang , Wang Shunqing , Harrington Christina A. , Qian David Z. , Sun Xiao‐Xin , Dai Mu‐Shui 
Published: January 2018

AbstractFOSL1 is frequently overexpressed in multiple types of human cancers including invasive breast cancers and implicated in cancer invasion and metastasis. However, how FOSL1 is overexpressed in cancers remains to be elucidated. Several microRNAs (miRNAs) have been shown to target FOSL1 and are downregulated in human cancers. Here, we report that miR‐130a is a novel FOSL1 targeting miRNA. Using gene expression microarray analysis, we found that FOSL1 is among the most up‐regulated genes in cells transfected with miR‐130a inhibitors. Transient transfection‐immunoblot, RNA‐immunoprecipitation, and luciferase reporter assays revealed that miR‐130a directly targets FOSL1 mRNA at its 3′‐UTR. Overexpression of miR‐130a significantly reduced the levels of FOSL1 in invasive breast cancer MDA‐MB‐231 and Hs578T cell lines and suppresses their migration and invasion. This inhibition can be rescued by ectopic expression of miR‐130a‐resistant FOSL1. Interestingly, we show that overexpression of miR‐130a increased the levels of tight‐junction protein ZO‐1 while inhibition of miR‐130a reduced the levels of ZO‐1. We further show that miR‐130a expression is significantly reduced in cancer tissues from triple‐negative breast cancer (TNBC) patients, correlating significantly with the upregulation of FOSL1 expression, compared to non‐TNBC tissues. Together, our results reveal that miR‐130a directly targets FOSL1 and suppresses the inhibition of ZO‐1, thus inhibiting cancer cell migration and invasion, in TNBCs.
A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis, and induces apoptosis in human colon epithelial cancer cells
Authors: Hegde Prajna , Rajakumar Sindhura B. , Swamy Bale M. , Inamdar Shashikala R. 
Published: January 2018

AbstractThe correlation between colorectal cancer (CRC) progression and altered expression of N‐glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N‐glycan specific mitogenic lectin from Rhizoctonia bataticola (RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here, we report the effect of RBL on human colon cancer HT 29, SW480, and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404, and 192, respectively for HT 29, SW480, and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC50 of 5, 6.4, and 6.8 µg/mL, respectively for HT 29, SW480, and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1%, 14.3%, and 23.2%, respectively at 12, 24, and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion, and migration assays demonstrated anti‐metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent.
Lysine‐specific demethylase 1 activation by vitamin B2 attenuates efficacy of apatinib for proliferation and migration of gastric cancer cell MGC‐803
Authors: Ma Jin‐Lian , Zhang Ting , Suo Feng‐Zhi , Chang Jiao , Wan Xiang‐Bin , Feng Xue‐Jian , Zheng Yi‐Chao , Liu Hong‐Min 
Published: January 2018

AbstractB vitamins play an essential role in the biosynthesis of nucleotides, replication of DNA, supply of methyl‐groups, growth and repair of cells, aberrancies of which have all been implicated in carcinogenesis. Although the potential role of vitamin B in relation to the risk of cancer, including breast, and colorectal cancer, has been investigated in several observational studies, the mechanism of action is still unclear. In this study, vitamin B2 exhibited efficient activation of LSD1 by occupying the active sites where FAD stands. Interestingly, vitamin B2 significantly downregulated expression of CD86, a sensitive surrogate biomarker of LSD1 inhibition, and showed marked activation of gastric cancer cell migration and invasion. Meanwhile, vitamin B2 induced activation of LSD1 may attenuate the proliferation inhibition, and anti‐migration effects of apatinib in gastric cancer cells. These findings suggested that vitamin B supplementation may interfere with the efficacy of apatinib in patients with gastric cancer.
Mechanical regulation of breast cancer migration and apoptosis via direct and indirect osteocyte signaling
Authors: Ma Yu‐Heng V. , Lam Candy , Dalmia Shreyash , Gao Peter , Young Jacob , Middleton Kevin , Liu Chao , Xu Henry , You Lidan 
Published: January 2018

AbstractBone metastases, the migration of cancers to bone, occur in 65‐80% of patients with advanced breast cancer. Metastasized cancer cells interact with cells such as the bone‐resorbing osteoclasts to alter bone remodeling. Exercise, often suggested as an intervention for cancer patients, regulates bone remodeling via osteocytes. Osteocytes also signal to endothelial cells, which may affect cancer cell extravasation. Therefore, we hypothesize that mechanically stimulated osteocytes can regulate processes in breast cancer bone metastasis. To test this, we exposed osteocytes to oscillatory fluid flow in vitro using parallel‐plate flow chambers. We observed that conditioned medium from flow‐stimulated osteocytes increased migration (by 45%) and reduced apoptosis (by 12%) of breast cancer cells. Conditioned medium from osteoclasts conditioned in flowed osteocytes' conditioned medium reduced migration (by 47%) and increased apoptosis (by 55%) of cancer cells. Cancer cell trans‐endothelial migration was reduced by 34% toward flowed osteocytes' conditioned medium. This difference was abolished with ICAM‐1 or IL‐6 neutralizing antibodies. Conditioned medium from endothelial cells conditioned in flowed osteocytes' conditioned medium increased cancer cell apoptosis by 29%. To summarize, this study demonstrated mechanically stimulated osteocytes' potential to affect breast cancer cells not only through direct signaling, but also through osteoclasts and endothelial cells. The anti‐metastatic potential of the indirect signalings is particularly exciting since osteocytes are further away from metastasizing cancer cells than osteoclasts and endothelial cells. Future studies into the effect of bone mechanical loading on metastases and its mechanism will assist in designing cancer intervention programs that lowers the risk for bone metastases.
Biological nitrification in the presence of sulfide and organic matter: effect of zeolite on the process in a batch system
Authors: Huiliñir Cesar , Medina Rodrigo , Montalvo Silvio , Castillo Alejandra , Guerrero Lorna 
Published: January 2018

AbstractBACKGROUNDEven though the nitrification process is inhibited in the presence of sulfide and organic matter, few efforts have been reported to overcome this weakness. The present work has the goal of determining the effect of zeolite (2.5, 5, 10 and 15 g L‐1) as an improver of the nitrification process in the presence of sulfide and organic matter in a batch system.RESULTSThe inhibitory effect of both sulfide and organic matter on the nitrification process was decreased by 53% when using zeolite, with the best results at a zeolite concentration of 15 g L‐1. No total ammonia nitrogen (TAN) adsorption on the zeolite was observed under these conditions, with TAN removal taking place mainly by biological oxidation. Specific nitrate production rate increased by 37.73% compared with the control. Sulfide was oxidized chemically in all the assays, with sulfate as the main product, while COD reduction and VSS production were not affected by zeolite.CONCLUSIONSZeolite can be used to improve nitrification in batch systems in the presence of sulfur and organic matter, obtaining a faster TAN removal rate and higher specific nitrate production compared with the controls. © 2018 Society of Chemical Industry
High cell‐density cultivation of phenolic acid decarboxylase‐expressing Escherichia coli and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis
Authors: Chen Yuheng , Li Lulu , Long Liangkun , Ding Shaojun 
Published: January 2018

AbstractBACKGROUND4‐vinyl guaiacol (4‐VG) is a high value‐added product widely used in the cosmetic, pharmaceutical, and chemical industries. The practical bioproduction of 4‐VG using phenolic acid decarboxylases has been limited by its relatively high biocatalyst cost and low yield and product concentration.RESULTSIn the present study, high‐cell density cultivation was employed to improve the activity and production of phenolic acid decarboxylase from Bacillus licheniformis (BLPAD) in recombinant Escherichia coli. The factors influencing enzyme production in E. coli such as the induction point and temperature for induction and feeding strategies were optimized. The highest BLPAD activity (531 U mL‐1) and productivity (20.4 U mL‐1 h‐1), respectively, were achieved in a 5 L bioreactor using a glucose exponential feeding strategy with isopropyl β‐D‐thiogalactoside (IPTG) as inducer. Furthermore, a high BLPAD production level (512 U mL‐1) was achieved using lactose as an inducer and continuous lactose feeding. Using a biphasic emulsion system with equal volumes (1 L) of cyclohexane as a organic solvent and a substrate fed‐batch strategy, the concentration and conversion yield of 4‐VG reached 129.9 g L‐1 (85.6%) in a 5 L bioreactor by whole‐cell biocatalysis, which is the highest reported to date.CONCLUSIONThis study describes a strategy for large‐scale 4‐VG bioproduction using the biocatalytic method. © 2018 Society of Chemical Industry
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface‐active compounds
Authors: Martins Margarida , Ooi Chien Wei , Neves Márcia C , Pereira Jorge FB , Coutinho João AP , Ventura Sónia PM 
Published: January 2018

AbstractBACKGROUNDGreen fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) producing the protein intracellularly. However, the common methods of extraction are cumbersome leading to an increase in the downstream process costs and complexity, sometimes leading to a higher risk of biomolecule degradation.RESULTSThis work proposes a new method to extract recombinant intracellular GFP from E. coli BL21 by using aqueous solutions of surface‐active compounds.CONCLUSIONBy comparing the fluorescence intensity of the extracted GFP, it was concluded that some of these compounds, namely ionic liquids with an alkyl chain of 10 or more carbons (best solvent being tributyl‐1‐tetradecylphosphonium, [P4,4,4,14]Cl) are more effective than an ultrasonic‐assisted extraction, even at low concentrations, being able to extract the whole GFP content from the cells. © 2018 Society of Chemical Industry