bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-Culture of Plant Beneficial Microbes as Source of Bioactive Metabolites
Authors: Vinale F. , Nicoletti R. , Borrelli F. , Mangoni A. , Parisi O. A. , Marra R. , Lombardi N. , Lacatena F. , Grauso L. , Finizio S. , Lorito M. , Woo S. L. 
Published: October 2017

AbstractIn microbial cultures the production of secondary metabolites is affected by experimental conditions, and the discovery of novel compounds is often prevented by the re-isolation of known metabolites. To limit this, it is possible to cultivate microorganisms by simulating naturally occurring interactions, where microbes co-exist in complex communities. In this work, co-culturing experiments of the biocontrol agent Trichoderma harzianum M10 and the endophyte Talaromyces pinophilus F36CF have been performed to elicit the expression of genes which are not transcribed in standard laboratory assays. Metabolomic analysis revealed that the co-culture induced the accumulation of siderophores for both fungi, while production of M10 harzianic and iso-harzianic acids was not affected by F36CF. Conversely, metabolites of the latter strain, 3-O-methylfunicone and herquline B, were less abundant when M10 was present. A novel compound, hereby named harziaphilic acid, was isolated from fungal co-cultures, and fully characterized. Moreover, harzianic and harziaphilic acids did not affect viability of colorectal cancer and healthy colonic epithelial cells, but selectively reduced cancer cell proliferation. Our results demonstrated that the co-cultivation of plant beneficial fungi may represent an effective strategy to modulate the production of bioactive metabolites and possibly identify novel compounds.
Sweetness induces sleep through gustatory signalling independent of nutritional value in a starved fruit fly
Authors: Hasegawa Tatsuya , Tomita Jun , Hashimoto Rina , Ueno Taro , Kume Shoen , Kume Kazuhiko 
Published: October 2017

AbstractStarvation reduces sleep in various animal species including humans and fruit flies. Immediate hunger and the following insufficient nutritional status resulting from starvation may affect sleep and arousal differently. In order to clarify the mechanism underlying the relationship between diet and sleep, we analysed the sleep behaviour of Drosophila melanogaster that were either starved or fed with different types of sugars. Starved flies showed longer activity bouts, short sleep bouts and a decreased arousal threshold. Non-nutritive sweeteners such as sucralose and arabinose, which are sweet but not nutritive, induced sleep in starved flies, but sleep bout length and the arousal threshold was short and decreased, respectively. On the other hand, sorbitol, which is not sweet but nutritive, did not induce sleep, but slightly increased the lowered arousal threshold. Activation of sweetness receptor expressing neurons induced sleep in starved flies. These results suggest that sweetness alone is sufficient to induce sleep in starved flies and that the nutritional status affects sleep homeostasis by decreasing the arousal threshold, which resulted in short sleep bouts in Drosophila.
India Is Overtaking China as the World’s Largest Emitter of Anthropogenic Sulfur Dioxide
Authors: Li Can , McLinden Chris , Fioletov Vitali , Krotkov Nickolay , Carn Simon , Joiner Joanna , Streets David , He Hao , Ren Xinrong , Li Zhanqing , Dickerson Russell R. 
Published: October 2017

AbstractSevere haze is a major public health concern in China and India. Both countries rely heavily on coal for energy, and sulfur dioxide (SO2) emitted from coal-fired power plants and industry is a major pollutant contributing to their air quality problems. Timely, accurate information on SO2 sources is a required input to air quality models for pollution prediction and mitigation. However, such information has been difficult to obtain for these two countries, as fast-paced changes in economy and environmental regulations have often led to unforeseen emission changes. Here we use satellite observations to show that China and India are on opposite trajectories for sulfurous pollution. Since 2007, emissions in China have declined by 75% while those in India have increased by 50%. With these changes, India is now surpassing China as the world’s largest emitter of anthropogenic SO2. This finding, not predicted by emission scenarios, suggests effective SO2 control in China and lack thereof in India. Despite this, haze remains severe in China, indicating the importance of reducing emissions of other pollutants. In India, ~33 million people now live in areas with substantial SO2 pollution. Continued growth in emissions will adversely affect more people and further exacerbate morbidity and mortality.
Post-drought hydraulic recovery is accompanied by non-structural carbohydrate depletion in the stem wood of Norway spruce saplings
Authors: Tomasella Martina , Häberle Karl-Heinz , Nardini Andrea , Hesse Benjamin , Machlet Anna , Matyssek Rainer 
Published: October 2017

AbstractHydraulic failure and carbon starvation are recognized as main causes of drought-induced forest decline. As water transport and carbon dynamics are strictly interdependent, it is necessary to clarify how dehydration-rehydration cycles are affecting the relations between stem embolism and non-structural carbohydrates (NSC). This is particularly needed for conifers whose embolism repair capability is still controversial. Potted Norway spruce saplings underwent two drought-re-irrigation cycles of same intensity, but performed in two consecutive summers. During the second cycle, stem percent loss of hydraulic conductivity (PLC) and NSC content showed no carry-over effects from the previous drought, indicating complete long-term recovery. The second drought treatment induced moderate PLC (20%) and did not affect total NSCs content, while starch was converted to soluble sugars in the bark. After one week of re-irrigation, PLC recovered to pre-stress values (0%) and NSCs were depleted, only in the wood, by about 30%. Our data suggest that spruce can repair xylem embolism and that, when water is newly available, NSCs stored in xylem parenchyma can be mobilized over short term to sustain respiration and/or for processes involved in xylem transport restoration. This, however, might imply dependency on sapwood NSC reserves for survival, especially if frequent drought spells occur.
KRAS mutation and primary tumor location do not affect efficacy of bevacizumab-containing chemotherapy in stagae IV colorectal cancer patients
Authors: Sun De-cong , Shi Yan , Wang Yan-rong , Lv Yao , Yan Huan , Mao Hui , Wang Zhi-kuan , Wu Zhi-yong , Shi Wei-wei , Dai Guang-hai 
Published: October 2017

AbstractThis study aims to investigate the efficacy of bevacizumab-combined chemotherapy (BCC) in Chinese stage IV colorectal cancer (CRC), and analyze the relationship between clinicopathological features with survival. Patients with stage IV CRC treated with BCC were analyzed retrospectively. 217 metastatic CRC (mCRC) patients were collected, out of which79 were right-sided CRCs and 138 were left-sided ones. Patients with Eastern Cooperative Oncology Group (ECOG) performance status ≤2, single agent chemotherapy, poor/mucous/signet ring cell component, second-and further-line of bevacizumab administration, multiple metastasis sites had comparatively worse survival. Among 141 patients with known KRAS status, 55 patients harbored KRAS mutation and 86 had wild type KRAS. The ORR and DCR were 41.9% and 78.9%, respectively, in patients with wild type KRAS, while ORR and DCR was 38.7% and 77.9%, respectively, in patients with KRAS mutation. The median PFS of patients with wild type and mutant KRAS were 8.38, and9.59 months, respectively; whereas the OS was 23.00 and 21.26 months, respectively for mCRC patients with wild-type and mutant KRAS. Cumulatively, our study indicated that BCC was effective and beneficial for Chinese stage IV CRC patients. KRAS mutation status and tumor location were not a prognostic factor for survival.
Descendants of the Jurassic turiasaurs from Iberia found refuge in the Early Cretaceous of western USA
Authors: Royo-Torres Rafael , Upchurch Paul , Kirkland James I. , DeBlieux Donald D. , Foster John R. , Cobos Alberto , Alcalá Luis 
Published: October 2017

AbstractA new, largely complete eusauropod dinosaur with cranial and postcranial elements from two skeletons, Mierasaurus bobyoungi gen. nov., sp. nov. from the lower Yellow Cat Member (Early Cretaceous) of Utah (USA), is the first recognized member of Turiasauria from North America. Moreover, according to our phylogenetic results, Moabosaurus utahensis from the lower Yellow Cat Member of Utah (USA) is also a member of this clade. This group of non-neosauropod eusauropods, which now includes five genera (Losillasaurus, Turiasaurus, Mierasaurus, Moabosaurus and Zby), was previously known only from the Jurassic of Europe. These recent discoveries in Utah suggest that turiasaurs as a lineage survived the Jurassic-Cretaceous extinction boundary and expanded their known range, at least, into western North America. The revised spatiotemporal distribution of turiasaurs is consistent with the presence of a land connection between North America and Europe sometime during the late Tithonian to Valanginian (c.147-133 Ma). Mierasaurus and Moabosaurus are the only non-neosauropod eusauropods known from North America, despite being younger than the classic neosauropods of the Morrison Formation (c.150 Ma).
Cereal products derived from wheat, sorghum, rice and oats alter the infant gut microbiota in vitro
Authors: Gamage Hasinika K. A. H. , Tetu Sasha G. , Chong Raymond W. W. , Ashton John , Packer Nicolle H. , Paulsen Ian T. 
Published: October 2017

AbstractThe introduction of different nutrient and energy sources during weaning leads to significant changes in the infant gut microbiota. We used an in vitro infant digestive and gut microbiota model system to investigate the effect of four commercially available cereal products based on either wheat, sorghum, rice or oats, on the gut microbiota of six infants. Our results indicated cereal additions induced numerous changes in the gut microbiota composition. The relative abundance of bacterial families associated with fibre degradation, Bacteroidaceae, Bifidobacteriaceae, Lactobacillaceae, Prevotellaceae, Ruminococcaceae and Veillonellaceae increased, whilst the abundance of Enterobacteriaceae decreased with cereal additions. Corresponding changes in the production of SCFAs showed higher concentrations of acetate following all cereal additions, whilst, propionate and butyrate varied between specific cereal additions. These cereal-specific variations in the concentrations of SCFAs showed a moderate correlation with the relative abundance of potential SCFA-producing bacterial families. Overall, our results demonstrated clear shifts in the abundance of bacterial groups associated with weaning and an increase in the production of SCFAs following cereal additions.
Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model
Authors: Raghunathan VijayKrishna , Eaton J. Seth , Christian Brian J. , Morgan Joshua T. , Ver Hoeve James N. , Yang Chen-Yuan Charlie , Gong Haiyan , Rasmussen Carol A. , Miller Paul E. , Russell Paul , Nork T. Michael , Murphy Christopher J. 
Published: October 2017

AbstractLaser-induced experimental glaucoma (ExGl) in non-human primates (NHPs) is a common animal model for ocular drug development. While many features of human hypertensive glaucoma are replicated in this model, structural and functional changes in the unlasered portions of trabecular meshwork (TM) of laser-treated primate eyes are understudied. We studied NHPs with ExGl of several years duration. As expected, ExGl eyes exhibited selective reductions of the retinal nerve fiber layer that correlate with electrophysiologic measures documenting a link between morphologic and elctrophysiologic endpoints. Softening of unlasered TM in ExGl eyes compared to untreated controls was observed. The degree of TM softening was consistent, regardless of pre-mortem clinical findings including severity of IOP elevation, retinal nerve fiber layer thinning, or electrodiagnostic findings. Importantly, this softening is contrary to TM stiffening reported in glaucomatous human eyes. Furthermore, microscopic analysis of unlasered TM from eyes with ExGl demonstrated TM thinning with collapse of Schlemm’s canal; and proteomic analysis confirmed downregulation of metabolic and structural proteins. These data demonstrate unexpected and compensatory changes involving the TM in the NHP model of ExGl. The data suggest that compensatory mechanisms exist in normal animals and respond to elevated IOP through softening of the meshwork to increase outflow.
Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative
Authors: Goh Wei Jiang , Zou Shui , Ong Wei Yi , Torta Federico , Alexandra Alvarez Fernandez , Schiffelers Raymond M. , Storm Gert , Wang Jiong-Wei , Czarny Bertrand , Pastorin Giorgia 
Published: October 2017

AbstractCell Derived Nanovesicles (CDNs) have been developed from the rapidly expanding field of exosomes, representing a class of bioinspired Drug Delivery Systems (DDS). However, translation to clinical applications is limited by the low yield and multi-step approach in isolating naturally secreted exosomes. Here, we show the first demonstration of a simple and rapid production method of CDNs using spin cups via a cell shearing approach, which offers clear advantages in terms of yield and cost-effectiveness over both traditional exosomes isolation, and also existing CDNs fabrication techniques. The CDNs obtained were of a higher protein yield and showed similarities in terms of physical characterization, protein and lipid analysis to both exosomes and CDNs previously reported in the literature. In addition, we investigated the mechanisms of cellular uptake of CDNs in vitro and their biodistribution in an in vivo mouse tumour model. Colocalization of the CDNs at the tumour site in a cancer mouse model was demonstrated, highlighting the potential for CDNs as anti-cancer strategy. Taken together, the results suggest that CDNs could provide a cost-effective alternative to exosomes as an ideal drug nanocarrier.
The necessity to choose causes reward-related anticipatory biasing: Parieto-occipital alpha-band oscillations reveal suppression of low-value targets
Authors: Heuer Anna , Wolf Christian , Schütz Alexander C. , Schubö Anna 
Published: October 2017

AbstractPositive outcome of actions can be maximized by choosing the option with the highest reward. For saccades, it has recently been suggested that the necessity to choose is, in fact, an important factor mediating reward effects: latencies to single low-reward targets increased with an increasing proportion of interleaved choice-trials, in which participants were free to choose between two targets to obtain either a high or low reward. Here, we replicate this finding for manual responses, demonstrating that this effect of choice is a more general, effector-independent phenomenon. Oscillatory activity in the alpha and beta band in the preparatory period preceding target onset was analysed for a parieto-occipital and a centrolateral region of interest to identify an anticipatory neural biasing mechanism related to visuospatial attention or motor preparation. When the proportion of interleaved choices was high, an increase in lateralized posterior alpha power indicated that the hemifield associated with a low reward was suppressed in preparation for reward-maximizing target selection. The larger the individual increase in lateralized alpha power, the slower the reaction times to low-reward targets. At a broader level, these findings support the notion that reward only affects responses when behaviour can be optimized to maximize positive outcome.
A Fully-Automatic Multiparametric Radiomics Model: Towards Reproducible and Prognostic Imaging Signature for Prediction of Overall Survival in Glioblastoma Multiforme
Authors: Li Qihua , Bai Hongmin , Chen Yinsheng , Sun Qiuchang , Liu Lei , Zhou Sijie , Wang Guoliang , Liang Chaofeng , Li Zhi-Cheng 
Published: October 2017

AbstractIn fully-automatic radiomics model for predicting overall survival (OS) of glioblastoma multiforme (GBM) patients, the effect of image standardization parameters such as voxel size, quantization method and gray level on model reproducibility and prognostic performance are still unclear. In this study, 45792 multiregional radiomics features were automatically extracted from multi-modality MR images with different voxel sizes, quantization methods, and gray levels. The feature reproducibility and prognostic performance were assessed. Multiparametric and fixed-parameter radiomics signatures were constructed based on a training cohort (60 patients). In an independent validation cohort (32 patients), the multiparametric signature achieved better performance for OS prediction (C-Index = 0.705, 95% CI: 0.672, 0.738) and significant stratification of patients into high- and low-risk groups (P = 0.0040, HR = 3.29, 95% CI: 1.40, 7.70), which outperformed the fixed-parameter signatures and conventional factors such as age, Karnofsky Performance Score and tumor volume. This study demonstrated that voxel size, quantization method and gray level had influence on reproducibility and prognosis of radiomics features for GBM OS prediction. An automatic method to determine the optimal parameter settings was provided. It indicated that multiparametric radiomics signature had the potential of offering better prognostic performance than fixed-parameter signatures.
A generic phase between disordered Weyl semimetal and diffusive metal
Authors: Su Ying , Wang X. S. , Wang X. R. 
Published: October 2017

AbstractQuantum phase transitions of three-dimensional (3D) Weyl semimetals (WSMs) subject to uncorrelated on-site disorder are investigated through quantum conductance calculations and finite-size scaling of localization length. Contrary to previous claims that a direct transition from a WSM to a diffusive metal (DM) occurs, an intermediate phase of Chern insulator (CI) between the two distinct metallic phases should exist due to internode scattering that is comparable to intranode scattering. The critical exponent of localization length is ν$$\,\simeq $$

≃
1.3 for both the WSM-CI and CI-DM transitions, in the same universality class of 3D Gaussian unitary ensemble of the Anderson localization transition. The CI phase is confirmed by quantized nonzero Hall conductances in the bulk insulating phase established by localization length calculations. The disorder-induced various plateau-plateau transitions in both the WSM and CI phases are observed and explained by the self-consistent Born approximation. Furthermore, we clarify that the occurrence of zero density of states at Weyl nodes is not a good criterion for the disordered WSM, and there is no fundamental principle to support the hypothesis of divergence of localization length at the WSM-DM transition.
Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody
Authors: Park Hyunjoon , Kim Minjae , Kim Hye-Jin , Lee Yeonjin , Seo Youngsil , Pham Chuong D. , Lee Joungmin , Byun Sung June , Kwon Myung-Hee 
Published: October 2017

AbstractA subset of monoclonal anti-DNA autoantibodies enters a variety of living cells. Here, we aimed to identify the endocytic receptors recognized by an internalizing anti-nucleic acid autoantibody, the 3D8 single-chain variable fragment (scFv). We found that cell surface binding and internalization of 3D8 scFv were inhibited markedly in soluble heparan sulfate (HS)/chondroitin sulfate (CS)-deficient or -removed cells and in the presence of soluble HS and CS. 3D8 scFv colocalized intracellularly with either HS proteoglycans (HSPGs) or CSPGs in HeLa cells. 3D8 scFv was co-endocytosed and co-precipitated with representative individual HSPG and CSPG molecules: syndecan-2 (a transmembrane HSPG), glypican-3 (a glycosylphosphatidylinositol (GPI)-anchored HSPG); CD44 (a transmembrane CSPG); and brevican (a GPI-anchored CSPG). Collected data indicate that 3D8 scFv binds to the negatively charged sugar chains of both HSPGs and CSPGs and is then internalized along with these molecules, irrespective of how these proteoglycans are associated with the cell membrane. This is the first study to show that anti-DNA antibodies enter cells via both HSPGs and CSPGs simultaneously. The data may aid understanding of endocytic receptors that bind anti-DNA autoantibodies. The study also provides insight into potential cell membrane targets for macromolecular delivery.
Microparticles controllable accumulation, arrangement, and spatial shaping performed by tapered-fiber-based laser-induced convection flow
Authors: Zhang Yu , Lei Jiaojie , Zhang Yaxun , Liu Zhihai , Zhang Jianzhong , Yang Xinghua , Yang Jun , Yuan Libo 
Published: October 2017

AbstractThe ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spatial resolution necessary to manipulate individual particle. Several approaches have been proposed that combine both high spatial selectivity and high throughput simultaneously. However, those methods are complex and difficult to fabricate. In this article, we propose and demonstrate a simple method that combines the laser-induced convection flow and fiber-based optical trapping methods to perform both regular and special spatial shaping arrangement. Essentially, we combine a light field with a large optical intensity gradient distribution and a thermal field with a large temperature gradient distribution to perform the microparticles shaping arrangement. The tapered-fiber-based laser-induced convection flow provides not only the batch manipulation of massive particles, but also the finer manipulation of special one or several particles, which break out the limit of single-fiber-based massive/individual particles photothermal manipulation. The combination technique allows for microparticles quick accumulation, single-layer and multilayer arrangement; special spatial shaping arrangement/adjustment, and microparticles sorting.
Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014–2015
Authors: Atkinson Kate V. , Bishop Lisa A. , Rhodes Glenn , Salez Nicolas , McEwan Neil R. , Hegarty Matthew J. , Robey Julie , Harding Nicola , Wetherell Simon , Lauder Robert M. , Pickup Roger W. , Wilkinson Mark , Gatherer Derek 
Published: October 2017

AbstractNasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014–2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.