bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract P335: An innate immune component in hypertension-induced cardiac dysfunction Christoph Mora, Katherine Deck, Yunmeng Liu, Lance Benson, Tonya Rafferty, & Shengyu Mu
Authors: Mora Christoph , Deck Katherine , Liu Yunmeng , Mu Shengyu , Benson Lance , Guo Yunping 
Published: October 2024

Hypertension affects nearly half of American adults, inevitably leading to cardiac dysfunction and heart failure. Cardiac fibrosis is a major factor contributing to cardiac dysfunction during hypertension progression. However, the mechanisms involved in cardio-fibrogenesis remain to be elucidated. In the past decade, studies revealed that macrophages could differentiate into professional fibrotic cells, myofibroblasts, through a process termed macrophage to myofibroblast transition (MMT), helping maintain the integrity of major organs, such as the kidney. However, it is unknown whether MMT contributes to fibrotic remodeling in hypertensive hearts. Here, we aim to identify this phenomenon in the hearts of hypertensive animals and determine the role it plays in the pathological progression from hypertension to cardiac diastolic dysfunction.
In this study we adopt the classic DOCA + Salt model of hypertension and utilized cardiac ultrasound and direct left ventricular (LV) pressure catheterization to gauge diastolic function. Heart sections from hypertensive and sham mice were stained with Masson’s trichrome to assess the extent of cardiac fibrosis. Furthermore, flow cytometry was utilized to identify MMT cells, which express both CD68 (monocytes) and α-SMA (myofibroblast) markers. These methods gave insight into the role that MMT plays in the degradation of heart function during hypertension.
Direct left ventricle pressure catheter revealed that DOCA + salt mice exhibited impaired diastolic function. This result is supported by cardiac ultrasound showing that the compliance of the heart had significantly decreased, as evidenced by the lowering of the efferent/atrial (E/A) ratio and deceleration time (DcT). In addition to this evidence of mechanical cardiac degradation, leukocytes isolated from the heart and stained with macrophage and myofibroblast markers revealed a significant increase in CD68+ & αSMA+ cells—a result reflected by increased fibrotic remodeling via Masson’s trichrome stain, in the hypertensive animals.
This study demonstrated an onset of diastolic dysfunction in the heart of DOCA + salt hypertensive mice. Moreover, we found a positive association between MMT cells and fibrosis in the hearts of hypertensive animals. Future studies will aim to parse out both the gravity of MMT in hypertensive heart dysfunction and the mechanism by which macrophages undergo this transition to find new targets against this debilitating disease.
Abstract P343: Cerebral injury and cognitive performances are correlated with immunoinflammatory markers in hypertensive patients
Authors: Carnevale Lorenzo , maffei angelo , Carnevale Daniela , Lembo Giuseppe 
Published: October 2024

Immunity and inflammation play a pivotal role in hypertension onset and cardiovascular organ damage. This role has been thoroughly characterized for classical hypertension targets as the kidneys, the heart and the vasculature. Many studies have characterized this in the experimental context, however immunoinflammatory challenges links with brain injury in the clinical context of hypertension are still lacking. In this study we will characterize how immunoinflammatory mediators can be predictors of cerebral injury characterized by advanced neuroimaging in hypertensive patients.
Hypertensive patients underwent cerebral MRI and by advanced neuroimaging we characterized the microstructural injury by DTI fiber tracking. We administered the Montreal Cognitive Assessment to evaluate cognitive function, measured circulating C-reactive protein (hs-CRP) and counted circulating white blood cells (WBC).
We found a large cluster of white matter tracts whose integrity parameters were associated with immunoinflammatory biomarkers (Figure A). In particular, a cluster of tracts of the limbic system and encompassing the corpus callosum show a correlation between their loss of integrity and hs-CRP levels. Another cluster of associative tracts spanning the temporal lobe show association between their loss of integrity and the WBC. Finally, the Frontal Aslant and the Uncinate Fasciculus show association with both immunoinflammatory markers (Figure B).
In this study we performed advanced neuroimaging analyses to characterize the interplay between immunoinflammatory risk and cerebral alterations in hypertensives. Furthermore, our previous work identified that white matter damage in the Forceps Minor and Superior Longitudinal Fasciculus was a typical trait of hypertensive patients and associated to loss of cognitive functions. Our data further show the inflammatory risk association with worse cognitive functions, suggesting a clinical relevance in terms of damage evidenced by advanced MRI in hypertensive patients.
Future Derecho Potential in the United States
Authors: Kaminski Kristie , Ashley Walker S. , Haberlie Alex M. , Gensini Vittorio A. 
Published: October 2024

Abstract
This study uses high-resolution, convection-permitting, dynamically downscaled regional climate simulation output to assess how long-lived, convectively induced, extratropical windstorms known as derechos may change across the CONUS during the twenty-first century. Three 15-yr epochs including a historical period (1990–2005) and two separate late-twenty-first-century periods (2085–2100) employing intermediate (RCP4.5) and pessimistic (RCP8.5) greenhouse gas concentration scenarios are evaluated. A mesoscale convective system (MCS) identification and tracking tool catalogs derecho candidates across epochs using simulated radar reflectivity and maximum 10-m wind speed as a proxy for near-surface severe wind gusts. Results indicate that MCS-based windstorms, including derechos, are more frequent, widespread, and intense in both future climate scenarios examined for most regions of the central and eastern CONUS. Increases are suggested across all parts of the year, with significant changes in populations concentrated during the early spring and summer months, suggesting the potential for a longer, more extreme MCS windstorm season. This research provides insights for forecasters, emergency managers, and wind-vulnerable stakeholders on how these events may change across the twenty-first century so that they may mitigate, adapt to, and become resilient against severe convective storm perils.

Significance Statement
Long-lived, thunderstorm-induced, damaging wind events known as derechos may increase across most portions of the central and eastern United States in the future, with projections indicating a near doubling or tripling of annual cases in the Midwest, eastern Great Plains, and Mississippi and Ohio Valley regions by the end of the twenty-first century. Modeling projections suggest that future derechos could generally be longer-lived, more expansive, and capable of producing more severe wind gusts and damage, which will ultimately increase risk to life, infrastructure, and wind-sensitive industries affected by these extreme thunderstorm events.
Using Generative AI to Promote Psychological, Feedback, and Artificial Intelligence Literacies in Undergraduate Psychology
Authors: Richmond Jenny L. , Nicholls Kate 
Published: October 2024

BackgroundWith the arrival of generative artificial intelligence (genAI) tools, psychology educators are rethinking their assessment practices.ObjectiveThis paper describes one approach to integrating genAI into an assessment designed to promote psychological literacy.MethodStudents used ChatGPT to generate a media release about a published article and then wrote a critique. We evaluated whether students were able to use the marking rubric to assess the ChatGPT output, and whether working with the rubric early in the assessment process had benefits for their grades on subsequent tasks.ResultsThe results show that students accurately assessed the ChatGPT output against the marking rubric, judging the output to be stylistically good but lacking in accurate coverage of the aims, methods, and results of the research. Working with genAI and the marking rubric early in the assessment process had benefits for performance, relative to cohorts that had engaged in peer review.ConclusionBy allowing students to use genAI and scaffolding the process of critiquing and revising, students gained competencies in psychological, feedback, and AI literacies.Teaching implicationsIntegrating genAI presents opportunities for learning, if educators can think beyond the artifact and design assessment that allows our students to showcase their learning process.
Generational Differences in Moral Injury, Resilience, and Well-Being Among Nurses: Predictors of Intention to Leave Position and Profession
Authors: Kim Minjin , Jun Jin , Lambert Joshua , Duah Henry , Tucker Sharon J. , O’Mathúna Dónal P. , Pignatiello Grant , Fitzpatrick Joyce J. 
Published: October 2024

Background: The COVID-19 pandemic highlighted the negative impact of moral injury on nurses’ well-being. However, there is a lack of research about generational differences among nurses, particularly on newer nurses who have been identified as having a higher rate of intention to leave. Objective: This study examines generational differences among nurses on moral injury, well-being, resilience, and intention to leave their nursing position and profession. Methods: This is a secondary analysis of cross-sectional data from registered nurses in clinical practice in Ohio between July and August 2021. Data on demographics, moral injury, resilience, and well-being were collected using an online survey. Participants were categorized into 4 generational groups based on their age in 2021: Baby Boomers (57-75 years old), Generation X (42-56 years old), Generation Y (27-41 years old), and Generation Z (12-26 years old). Descriptive and inferential statistics, including logistic regression and analysis of variance, were employed for analysis. Results: Significant generational differences were found in years of clinical experience, moral injury, resilience, and well-being. Baby Boomers reported higher well-being and resilience and lower moral injury. Notably, the intention to leave the profession was more strongly associated with well-being and moral injury levels than with the years of experience or generational group. Conclusions: The findings suggest that interventions to improve nurse retention should prioritize enhancing well-being and addressing the root causes of moral injury. Tailored strategies addressing the needs of different generations are necessary for mitigating the adverse effects of current healthcare challenges on nurse attrition.
Design and development of an eHealth intervention to support self-management in people with musculoskeletal disorders: ‘eHealth: it's TIME’
Authors: Kelly Marie , M. Fullen Brona , Martin Denis , Bradley Colin , O’Riain Eoghan , McVeigh Joseph G 
Published: October 2024

Objective This study aimed to co-design and develop a user-centred, theory-based eHealth-mediated self-management support follow-up prototype for adults with musculoskeletal disorders. Design A three-step system development cycle was employed. Step 1 involved creating intervention features and content, with two focus groups reviewing prioritised eHealth intervention elements based on earlier research. Step 2 involved heuristic testing using Nielsen's 10 heuristic principles. Step 3 incorporated qualitative think-aloud interviews and the System Usability Scale. Setting Republic of Ireland. Participants Step 1 included adults with musculoskeletal disorders ( n = 12). Step 2 involved five reviewers. Step 3 included people with musculoskeletal disorders ( n = 5) and musculoskeletal physiotherapists ( n = 5). Results Participants in step 1 approved four main intervention components, which map to recognised theoretical frameworks, and suggested increased use of visual and interactive elements. Heuristic testing in step 2 identified design and navigation issues. In Step 3, usability testing, additional navigation, content and design recommendations were identified. The overall median system usability score (interquartile range) was 75 (0) out of 100 for adults with musculoskeletal disorders and 77.5 (2.5) out of 100 for musculoskeletal physiotherapists, indicating good usability. Conclusion A theory-based, user-centred eHealth-mediated follow-up self-management support prototype has been developed for people with musculoskeletal disorders, with the next steps focusing on feasibility testing in clinical practice, with a more diverse population.
Rapid and sustained reduction of treatment-resistant PTSD symptoms after intravenous ketamine in a real-world, psychedelic paradigm
Authors: MacConnel Henry A , Earleywine Mitch , Radowitz Steven 
Published: October 2024

Background: Traditional treatments for post-traumatic stress disorder (PTSD) often show limited success with high dropout. Ketamine, an N-methyl-D-aspartate antagonist known for rapid antidepressant effects, has decreased PTSD symptoms in some studies but not in others. Administering ketamine in ways that parallel psychedelic-assisted treatments—including preparatory, integration, sensory immersion, and psychotherapy sessions—could decrease PTSD symptoms meaningfully. Methods: A retrospective sample of 117 screened outpatients with elevated PTSD Checklist for DSM-5 (PCL-5) scores received intravenous ketamine in supportive environments. The protocol included preparation, intention-setting, and integration sessions accompanying at least six administrations. Administration sessions included eye shades and evocative music paralleling typical psychedelic therapy trials. Results: Mean PCL scores decreased from 52.54 (SD = 12.01) to 28.78 (SD = 16.61), d = 1.64. Patients tolerated treatment well, with no serious adverse events. Covariates, including age, gender, days between PCL assessments, number of psychiatric medications, and suicidal ideation were not significant moderators; concomitant psychotherapy did reach significance, d = 0.51. Of the 117 patients’ final PCL scores, 88 (75.21%) measures suggested clinically meaningful improvement and 72 (61.54%) suggested remission of PTSD symptoms. Conclusion: Intravenous ketamine in supportive environments, with hallmarks of psychedelic therapy, preceded large reductions in PTSD symptoms. These results highlight ketamine’s potential when delivered in this manner, suggesting environmental factors might account for some variation seen in previous work. Given the molecule’s cost, minimal interaction with other psychiatric medications, and legal status, intravenous ketamine in a psychedelic paradigm may be a promising option for PTSD unresponsive to other treatments.
Influence of Platelet Concentration on the Clinical Outcome of Platelet-Rich Plasma Injections in Knee Osteoarthritis
Authors: Boffa Angelo , De Marziani Luca , Andriolo Luca , Di Martino Alessandro , Romandini Iacopo , Zaffagnini Stefano , Filardo Giuseppe 
Published: October 2024

Background: Platelet-rich plasma (PRP) is one of the most frequently used orthobiologic products for the injection treatment of patients affected by knee osteoarthritis (OA). Some preliminary evidence supports the influence of platelet concentration on patients’ clinical outcomes. Purpose: To analyze if platelet concentration can influence the safety and clinical efficacy of PRP injections for the treatment of patients with knee OA. Study Design: Cohort study; Level of evidence, 3. Methods: This study consisted of 253 patients with knee OA (142 men, 111 women; mean ± SD age, 54.8 ± 11.4 years; Kellgren-Lawrence grades 1-3) who were treated with 3 intra-articular injections of 5 mL of autologous leukocyte-rich or leukocyte-poor PRP. All patients were prospectively evaluated at baseline and at 2, 6, and 12 months. Patients were clinically assessed thorough the Knee injury and Osteoarthritis Outcome Score (KOOS) subscales and the International Knee Documentation Committee (IKDC) Subjective score. Platelet concentration was correlated with clinical outcome. Further analysis was performed by stratifying patients into 3 groups (homogeneous for OA severity) based on platelet concentration (high, medium, and low). All complications and adverse events were reported, as well as failures. Results: An overall statistically significant improvement in all clinical scores was documented from baseline to each follow-up evaluation. Platelet concentration positively correlated with clinical outcome. KOOS Pain improved more with higher platelet concentration at 2 months ( P = .036; rho = 0.132), 6 months ( P = .009; rho = 0.165), and 12 months ( P = .014; rho = 0.155). The same trend was shown by the other KOOS subscales and by the IKDC Subjective score, as well as by the comparison of the groups of high-, medium-, and low-platelet PRP. The highest failure rate (15.0%) was found in the low-platelet group as compared with the medium-platelet group (3.3%) and the high-platelet group (3.3%). No differences were observed among the 3 groups in terms of adverse events. Conclusion: This study demonstrated that platelet concentration influences the clinical outcome of PRP injections in knee OA treatment. PRP with a higher platelet concentration provides a lower failure rate and higher clinical improvement as compared with PRP with a lower platelet concentration, with overall better results up to 12 months of follow-up in patients with knee OA.
Extracorporeal membrane oxygenation ameliorate hepatic injury in brain death rat donors with hemodynamic instability
Authors: Yang Jianbao , Li Jian , Zhuoga Awang , Yu Zeyuan , Li Yongnan , Jiao Zuoyi 
Published: October 2024

Background: Donation after brain death (DBD) serves as the primary source for liver transplantation. However, livers obtained through DBD often incur damage due to unstable hemodynamics, potentially impacting transplantation outcomes. Extracorporeal Membrane Oxygenation (ECMO) emerges as an optimal technique for donor liver retrieval and has found application in clinical settings. Despite its clinical implementation, the precise mechanisms through which ECMO enhances liver functions remain elusive. This study aims to investigate the mechanisms underlying how ECMO ameliorates liver function in brain-dead donors. Methods: We randomly assigned 18 male Sprague-Dawley (SD) rats (350 ± 50 g) into three groups: Con ( n = 6), DBD-assisted drug ( n = 6), and DBD-assisted ECMO ( n = 6). After 3 h of ECMO, the rats were sacrificed. We assessed and compared changes in heart rate, blood pressure, cumulative liver damage (evaluated through HE and TUNEL staining), serum levels of AST and ALT, alterations in serum oxidative stress factors (MDA, H2O2, SOD, and 8-OHdG), and serum concentrations of related inflammatory factors (interleukin [IL]-1β, IL-6, IL-8, and TNF-α) among rats in the Con, DBD-assisted drug, and DBD-assisted ECMO groups. Subsequently, we established a rat orthotopic liver transplantation (OLT) model and transplanted livers obtained through the aforementioned methods. The post-transplantation status of the livers was observed. Results: After 3 h of brain death, liver injury worsened, accompanied by a significant increase in serum transaminases, inflammatory responses, oxidative stress, and TUNEL staining. Strikingly, ECMO not only stabilized hemodynamics after DBD but also mitigated liver damage, leading to an alleviated status post liver transplantation. Conclusions: ECMO stabilizes hemodynamics, attenuates inflammatory responses and oxidative stress, thereby enhancing the quality of liver grafts for transplantation.
Evaluating the impact of type 2 diabetes mellitus on interstitial lung disease prevalence in patients with systemic lupus erythematosus: A national inpatient sample analysis
Authors: Saliba Fares , Khattar Georges , Mourad Omar , Aoun Laurence , Bou Sanayeh Elie , Arafa Fatema , Al Saidi Ibrahim , Abidor Erica , Al Achkar Michel , Rizvi Taqi , Sangaraju Koushik , Di Pietro Gaetano , Haddadin Fadi , Almardini Shaza , El Gharib Khalil , El-Hage Halim 
Published: October 2024

Background Systemic lupus erythematosus (SLE) increases the risk of interstitial lung disease (ILD). SLE is also linked to an elevated risk of type 2 diabetes mellitus (T2DM). However, the impact of T2DM on ILD risk in patients with SLE is still unclear. This study aimed to compare the prevalence of ILD in patients with SLE based on the presence of T2DM (SLE + T2DM+) or its absence (SLE + T2DM−). Methods This was a retrospective cohort study using the 2019–2020 National Inpatient Sample database. Adult SLE patients were identified and stratified by T2DM status. Comparable cohorts were created using propensity score matching, resulting in 10,532 patients in each cohort. Multivariate logistic regression assessed the association between T2DM and ILD. Results T2DM was associated with a lower prevalence of ILD in patients with SLE (OR 0.798, 95% CI: 0.695–0.918, p = .002), occurring in 371 (3.5%) patients with T2DM compared to 463 (4.4%) patients without T2DM. Specifically, this difference was mainly driven by pulmonary fibrosis, which was significantly less frequent in the T2DM group (1.3% vs 1.8%, OR 0.7, 95% CI: 0.560–0.875, p = .002). No differences were found in secondary outcomes, including death rates, length of hospital stay, ARDS, pneumothorax, pleural effusion, or pulmonary arterial hypertension. Conclusion Our study suggests that T2DM significantly reduced ILD risk in patients with SLE, specifically diminishing pulmonary fibrosis prevalence. Further research should explore mechanisms for this protective association between T2DM and ILD development in SLE. These findings may guide management strategies for this vulnerable population.
Evaluation and Analysis of Traditional Chinese Medicine Treatment of Bovine Viral Diarrhea/Mucosal Disease Based on Network Pharmacology and In Vitro Studies
Authors: Chen Liang , Lan Shijie , Shen Sisi , Wang Jiahui , Liu Xuesong , Jiang Botao , Zhong Peng , Liu Bochao , Yao Shuang , Qin Pingwei , Feng Wanyu 
Published: October 2024

Background Bovine viral diarrhea/mucosal disease (BVD-MD) is widely distributed worldwide. The disease causes serious economic losses to animal husbandry every year. Finding targeted antiviral drugs proves to be an effective strategy. Purpose This study was based on network pharmacology and in vitro studies to analyze the potential of traditional Chinese medicine (TCM) in the treatment of BVD-MD. Methods The intersection targets between TCM and the disease were identified. Network topology analysis and protein–protein interaction (PPIs) networks were performed. Intersection targets were analyzed for gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. The molecular docking and molecular dynamics simulation methods were used to reveal the degree of binding of core components to key target genes. Characterization of the anti-Bovine viral diarrhea virus (BVDV) effect of TCM by cytotoxicity and in vitro studies. Results The results revealed the selection of five key Chinese medicines, along with 206 targets associated with BVD-MD. The toll-like receptor, PI3K-AKT, and tumor necrosis factor (TNF) signaling pathways were closely related to the five TCMs. AKT1, EGFR, HSP90AA1, and MAPK1 had good binding with quercetin, the core component of Chinese medicine. Molecular dynamics analysis showed that quercetin exhibited complex stability with AKT1. In vitro studies have demonstrated that the inhibitory effect of quercetin on BVDV is mainly in the initial phase. Quercetin inhibited the expression of AKT1. Conclusion The mechanism of BVDV inhibition by the core Chinese medicines is closely related to MAPK1, AKT1, TNF, and HSP90AA1, with the reduction of AKT1 ultimately affecting viral expression.