bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longer Telomere Length in Elite Master Sprinters: Relationship to Performance and Body Composition
Authors: Simoes Herbert , Sousa Caio , dos Santos Rosa Thiago , da Silva Aguiar Samuel , Deus Lysleine , Rosa Erica , Amato Angelica , Andrade Rosangela 
Published: November 2017

AbstractEmergent evidence suggests that the long-term healthy lifestyle of master athletes may attenuate aging. We compared telomere length (TL) of high-level master sprinters and non-athlete age-matched controls, and analyzed the relationships of TL with performance and body fat. Elite master sprinters (n=11; aged 50.1±9.2yrs) and healthy untrained controls (n=10; aged 45.4±10.9yrs) had blood samples collected for biochemical and biomolecular analyses. Master sprinters had longer TL, lower body fat and BMI, and a better lipid profile than age-matched controls (p<0.05). A large effect size was verified comparing TL between athletes vs. controls (Cohen’s d=1.039), with a significant negative correlation between TL and performance decline per decade (r=−0.624, p<0.01) and a positive correlation of TL and actual performance level (r=0.641, p<0.01). In conclusion, TL of elite master sprinters was longer than their untrained peers, and seems to be not only a marker of health status, but also an indicator of sports longevity since both actual performance level and its decrease over years were related to TL. Further research might assess the TL of elite master endurance athletes for comparison with sprinters, and also investigate the underlying mechanisms by which the attenuation of telomere shortening occurs in master athletes.
Multidetector Computed Tomography Angiography (MD-CTA) of Coronary Artery Bypass Grafts – Update 2017
Authors: Jungmann Florian , Emrich Tilman , Mildenberger Peter , Emrich Anna , Düber Christoph , Kreitner K. 
Published: November 2017

Background Coronary artery bypass grafting (CABG) is still an important therapeutic approach in the treatment especially of advanced coronary artery disease. In this study, we elucidate the current role of multidetector computed tomography angiography (MD-CTA) in imaging patients after CABG surgery.
Method This study is based on recent reports in the literature (2007 – 2016) on imaging of CABG using 64-slice MD-CT scanners and beyond. We included 13 reports that compared ECG-gated MD-CTA with conventional invasive coronary angiography (ICA) as the reference standard for the assessment of graft patency and for the detection of > 50 % stenoses. These studies had to provide absolute values for true-positive, true-negative, false-positive and false-negative results or at least allow calculation of these numbers. In total, 1002 patients with 2521 bypass grafts were the basis for this review.
Results and Conclusion The sensitivity and specificity for the assessment of graft patency or the detection of > 50 % graft stenosis were 97.2 % and 97.5 %, respectively. The negative and positive predictive values were 93.6 % and 99 %, respectively. By using prospective ECG-gating and an increasing pitch factor, the radiation dose exposure declined to 2.4 mSv in the latest reports. ECG-gated MD-CTA provides a fast and reliable, noninvasive method for assessing patients after CABG. The most substantial benefit of the newest CT scanner generations is a remarkable reduction of radiation dose exposure while maintaining a still excellent diagnostic accuracy during recent years.
Key Points
Citation Format
Structural Searching of Biosynthetic Enzymes to Predict Protein Targets of Natural Products
Authors: Sturm Noé , Quinn Ronald , Kellenberger Esther 
Published: November 2017

AbstractRecently, we have demonstrated that site comparison methodology using flavonoid biosynthetic enzymes as the query could automatically identify structural features common to different flavonoid-binding proteins, allowing for the identification of flavonoid targets such as protein kinases. With the aim of further validating the hypothesis that biosynthetic enzymes and therapeutic targets can contain a similar natural product imprint, we collected a set of 159 crystallographic structures representing 38 natural product biosynthetic enzymes by searching the Protein Databank. Each enzyme structure was used as a query to screen a repository of approximately 10 000 ligandable sites by active site similarity. We report a full analysis of the screening results and highlight three retrospective examples where the natural product validates the method, thereby revealing novel structural relationships between natural product biosynthetic enzymes and putative protein targets of the natural product. From a prospective perspective, our work provides a list of up to 64 potential novel targets for 25 well-characterized natural products.
“I want a real apology”
Authors: Rieger Caroline L. 
Published: November 2017

AbstractResearch on the apology spans over half a century and has been quite prolific. Yet, a major issue with numerous studies on apologies is a lack of findings from naturally occurring interaction. Instead many studies examine written elicitations. As a result they research how respondents think they apologize, not how they do apologize. This project, in contrast, stresses the importance of studying the apology as a dynamically constructed politeness strategy in situated interaction. Apologies are part of the ever-present relational work, i.e., co-constructed and co-negotiated, emergent relationships in a situated social context. Hence, the focus is not on the illocutionary force indicating device (IFID) alone, nor on the turn in which the IFID is produced, but on the interactional exchange in situ.Naturally, data eliciting produces a larger sample size of apologies than the taping and transcribing of naturally occurring interaction does. To remedy the issue, this study uses interactions from situation comedies, which provide a large sample of apologies in their interactional context. Sitcom interactions constitute a valid focus of pragmatic research as they share fundamental elements of natural interactions (B. Mills 2009;Quaglio 2009). The validity of this approach is tested using findings from published conversation analytic studies on apologies. The analysis is set within the framework of discursive pragmatics and leads to new insights on apologies and responses to apologies.
Discovering the assignment
Authors: de Groot Casper 
Published: November 2017

Abstract
This introductory chapter presents a search into the realm of the essive markers indicating impermanent state in the Uralic languages. It turns out that full or even partial descriptions of the distribution of the essive markers in most Uralic languages are lacking. Overall studies of the essive in Uralic describing similarities and differences among the languages are not available. In order to fill this gap the chapter presents a typological questionnaire which makes the appropriate description and comparison of the distribution of the essive in the various Uralic languages possible.
Identifying structural variants using linked-read sequencing data
Authors: Elyanow Rebecca , Wu Hsin-Ta , Raphael Benjamin J 
Published: November 2017

Abstract

Motivation
Structural variation, including large deletions, duplications, inversions, translocations and other rearrangements, is common in human and cancer genomes. A number of methods have been developed to identify structural variants from Illumina short-read sequencing data. However, reliable identification of structural variants remains challenging because many variants have breakpoints in repetitive regions of the genome and thus are difficult to identify with short reads. The recently developed linked-read sequencing technology from 10X Genomics combines a novel barcoding strategy with Illumina sequencing. This technology labels all reads that originate from a small number (∼5 to 10) DNA molecules ∼50 Kbp in length with the same molecular barcode. These barcoded reads contain long-range sequence information that is advantageous for identification of structural variants.

Results
We present Novel Adjacency Identification with Barcoded Reads (NAIBR), an algorithm to identify structural variants in linked-read sequencing data. NAIBR predicts novel adjacencies in an individual genome resulting from structural variants using a probabilistic model that combines multiple signals in barcoded reads. We show that NAIBR outperforms several existing methods for structural variant identification—including two recent methods that also analyze linked-reads—on simulated sequencing data and 10X whole-genome sequencing data from the NA12878 human genome and the HCC1954 breast cancer cell line. Several of the novel somatic structural variants identified in HCC1954 overlap known cancer genes.

Availability and implementation
Software is available at compbio.cs.brown.edu/software.

Supplementary information
Supplementary data are available at Bioinformatics online.
Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling
Authors: Green Jack P , Souilhol Celine , Xanthis Ioannis , Martinez-Campesino Laura , Bowden Neil P , Evans Paul C , Wilson Heather L 
Published: November 2017

Abstract

Objective
Atherosclerosis is a focal disease occurring at arterial sites of disturbed blood flow that generates low oscillating shear stress. Endothelial inflammatory signalling is enhanced at sites of disturbed flow via mechanisms that are incompletely understood. The influence of disturbed flow on endothelial adenosine triphosphate (ATP) receptors and downstream signalling was assessed.

Methods and results
Cultured human endothelial cells were exposed to atheroprotective (high uniform) or atheroprone (low oscillatory) shear stress for 72 h prior to assessment of ATP responses. Imaging of cells loaded with a calcium-sensitive fluorescent dye revealed that atheroprone flow enhanced extracellular calcium influx in response to 300 µM 2'(3')-O-(4-Benzoylbenzoyl) adenosine-5'-triphosphate. Pre-treatment with pharmacological inhibitors demonstrated that this process required purinergic P2X7 receptors. The mechanism involved altered expression of P2X7, which was induced by atheroprone flow conditions in cultured cells. Similarly, en face staining of the murine aorta revealed enriched P2X7 expression at an atheroprone site. Functional studies in cultured endothelial cells showed that atheroprone flow induced p38 phosphorylation and up-regulation of E-selectin and IL-8 secretion via a P2X7-dependent mechanism. Moreover, genetic deletion of P2X7 significantly reduced E-selectin at atheroprone regions of the murine aorta.

Conclusions
These findings reveal that P2X7 is regulated by shear forces leading to its accumulation at atheroprone sites that are exposed to disturbed patterns of blood flow. P2X7 promotes endothelial inflammation at atheroprone sites by transducing ATP signals into p38 activation. Thus P2X7 integrates vascular mechanical responses with purinergic signalling to promote endothelial dysfunction and may provide an attractive potential therapeutic target to prevent or reduce atherosclerosis.
Cost-Effectiveness of Primary and Revision Surgery for Adult Spinal Deformity
Authors: Raman Tina , Nayar Suresh K. , Liu Shuiqing , Skolasky Richard L. , Kebaish Khaled M. 
Published: November 2017

Study Design.
Retrospective comparative study.

Objective.
The purpose of this study is to compare functional outcomes, hospital resource utilization, and spine-related costs during 2 years in patients who had undergone primary or revision surgery for adult spinal deformity (ASD).

Summary of Background Data.
After surgery for ASD, patients may require revision for pseudarthrosis, implant complications, or deformity progression. Data evaluating cost-effectiveness of primary and, in particular, revision surgery, for ASD are sparse.

Methods.
We retrospectively reviewed records for 119 consecutive patients who had undergone primary or revision surgery for ASD. Two-year total spine-related medical costs were derived from hospital charge data. Functional outcome scores were extracted from prospectively collected patient data. Cost utility ratios (cost/quality-adjusted life-year [QALY]) at 2 years were calculated and assessed against a threshold of $154,458/QALY gained (three times the 2015 US per-capita gross domestic product).

Results.
The primary surgery cohort (n = 56) and revision cohort (n = 63) showed significant improvements in health-related quality-of-life scores at 2 years. Median surgical and spine-related 2-year follow-up costs were $137,990 (interquartile range [IQR], $84,186) for primary surgery and $115,509 (IQR, $63,753) for revision surgery and were not significantly different between the two groups (P = 0.12). We report 2-year QALY gains of 0.36 in the primary surgery cohort and 0.40 in the revision group (P = 0.71). Primary instrumented fusion was associated with a median 2-year cost per QALY of $197,809 (IQR, $187,350) versus $129,950 (IQR, $209,928) for revision surgery (P = 0.31).

Conclusion.
Revision surgery had lower total 2-year costs and higher QALY gains than primary surgery for ASD, although the differences were not significant. Although revision surgery for ASD is known to be technically challenging and to have a higher rate of major complications than primary surgery, revision surgery was cost-effective at 2 years. The cost/QALY ratio for primary surgery for ASD exceeded the threshold for cost effectiveness at 2 years.

Level of Evidence: 3
Comparisons of Self-reported Glaucoma Medication Adherence With a New Wireless Device: A Pilot Study
Authors: Gatwood Justin D. , Johnson Jordan , Jerkins Brian 
Published: November 2017

Purpose:
To compare use of topical medications between a wireless monitoring device and validated self-reported measures of glaucoma medication adherence.

Patients and Methods:
This study involved adults from a group ophthalmology practice diagnosed with and being medicinally treated for glaucoma who were not scheduled for a surgery during the study period. Subjects were required to use a new wireless device to dispense their glaucoma medication for 2 months, and were surveyed at baseline and immediately following the study to assess mobile phone use, glaucoma-related self-efficacy, and medication adherence.

Results:
Complete data (survey and accurate device recordings) were available for 23 subjects at both baseline and endpoint. Median adherence, as measured by the device, was 82% and dropped slightly between 30-day periods, from 83% to 77%. Similarly, the percent adherent (dosing at least 75% of the time) dropped significantly between months according to both the device (78.3% and 52.2%) and a self-reported measure (63% and 56%). Kappa statistics indicated low agreement between the device and self-report when classifying adherent status. A majority of subjects interviewed found the device easy to use, indicated that it did not interfere with medication-taking or normal activities, and were not bothered by their physician knowing when medication was dispensed.

Conclusion:
In this pilot, nearly all Kali Drop devices performed as expected, providing real-time data on medication use over a 60-day period. Data suggested that self-reported and electronic estimates of glaucoma medication use differ, but additional testing of this new device is needed to corroborate the data observed.
Isotretinoin-induced Angle Closure and Myopic Shift
Authors: Park Young-myoung , Lee Tae-Eun 
Published: November 2017

Purpose:
To report and describe the management a rare case of transient bilateral angle closure with increased intraocular pressure (IOP) and myopic shift while on isotretinoin therapy for acne.

Methods:
A 28-year-old woman presented with bilateral myopic shift, angle closure with IOP increase, and supraciliary effusion 1 week after acne therapy with isotretinoin. Two weeks after stopping isotretinoin, and treatment with topical prednisolone acetate, atropine, and fixed combination of timolol and dorzolamide, refraction, IOP returned to normal and supraciliary effusions was decreased on ultrasound biomicroscopy.

Conclusions:
Oral isotretinoin for acne treatment may be associated with an adverse reaction, resulting in bilateral transient myopia and angle closure with IOP elevation.
Assessment of a Health Promotion Model on Obese Turkish Children
Authors: Eren Fidanci Berna , Akbayrak Nalan , Arslan Filiz 
Published: November 2017

ABSTRACT

Background:
Studies of childhood obesity have shown that fostering healthy life behaviors is more important than losing weight. However, studies reporting this result did not use a theoretical nursing model, which offers a systematic approach and scientific basis for studying the concepts and principles of nursing practice.

Purpose:
The aim of this study was to determine the effects of an intervention that is based on Nola J. Pender’s Health Promotion Model (HPM) on the healthy life behaviors and self-confidence of obese children.

Methods:
This quasi-experimental study enrolled 86 obese children and their parents (48 in the experimental group and 38 in the control group) as participants. The data collection tool was composed of an adaptation of a Pender’s model-based evaluation form and the “Piers–Harris Self Confidence Scale.” The experimental group received HPM-based individual and group education, whereas the control group received routine follow-up only. Whereas qualitative data were analyzed using content analysis, quantitative data were analyzed using a paired t test and Mann–Whitney U or Wilcoxon test.

Results:
At the end of the education and follow-up program, experimental group participants showed a significant increase in healthy eating habits such as noting food portions (Z = 5.231, p < .001) and choosing water instead of sugary drinks (Z = 4.130, p < .001) and spent significantly less time per day in front of a television or computer (Z = 5.085, p < .001). Furthermore, after the intervention, experimental group participants had reduced their total body mass index standard deviation score (Z = 6.031, p < .001) and had an average self-confidence score that differed significantly from the control group (Z = 3.796, p < .001) and that was significantly higher than the average preintervention score (Z = 5.971, p < .01).

Conclusions:
Health professionals must recognize the physical, psychological, and social risks of obesity in children and focus on these problems to identify appropriate solutions. It is recommended that education and nursing follow-up programs be developed based on nursing models. This study found that patient education based on Pender’s HPM increased healthy life behaviors (e.g., healthy nutrition and increased exercise time) and decreased sedentary activity substantially in the experimental group. This study further suggests that, although Pender’s HPM has not been tested on obese children, this model offers effective guidelines for nursing activities that are targeted on developing healthy life behaviors in children.
Social Support and Health-Related Quality of Life Among Elderly Individuals Living Alone in South Korea: A Cross-Sectional Study
Authors: KIM Jiyun , LEE Jong-Eun 
Published: November 2017

ABSTRACT

Background:
Health-related quality of life (HRQoL) is an important component of healthy aging. Elderly individuals living alone with health risks have rapidly increased in South Korea. Inadequate social support has been associated with poor HRQoL in elderly individuals who are currently living alone. Although studies of HRQoL in elderly subjects who live alone have been conducted, studies of the relationships between social support and HRQoL have rarely targeted the elderly individuals living alone.

Purpose:
The aim of this study was to identify the factors that are related to HRQoL among the elderly individuals living alone in South Korea.

Methods:
A cross-sectional study design was used. Five hundred seventeen elderly individuals who were living alone in community dwellings and aged 65 years or older were included. The structured questionnaires included the 12-item Short-Form Health Survey, Multidimensional Scale of Perceived Social Support, demographic characteristics, health status, and behavior factors. A descriptive analysis and hierarchical multivariate logistic regression were conducted to examine significant correlations between the variables.

Results:
The mean score of the Physical Component Summary was 43.00 (± 4.82), and the mean score of the Mental Component Summary (MCS) was 45.94 (± 7.21). After controlling for demographic characteristics, health status, and behavior factors, support from significant others was negatively associated with the Physical Component Summary scores, whereas support from significant others, family, and friends was positively associated with the MCS scores.

Conclusions:
Social support emerged as an important factor in the HRQoL domains of the MCS of the participants. Future interventions should focus more on implementing effective social support strategies to improve HRQoL in elderly individuals who live alone.
Association of Proton Pump Inhibitor Use With Serum Biomarkers of Inflammation, Insulin Resistance, Cardiovascular Risk, and Renal Function
Authors: Austin Gregory L. , Weiskopf Jennifer R. , Czwornog Jennifer L. 
Published: November 2017

Background and Goals:
Proton pump inhibitor (PPI) use has been associated with cardiovascular disease, chronic kidney disease, and dementia. Prior studies did not account for key confounders and little is known about the association of PPIs with serum biomarkers of inflammation, insulin resistance, cardiovascular risk, and renal function. Our aims were to investigate differences in these biomarkers between PPI users and nonusers.

Methods:
Our data are from the National Health and Nutrition Examination Survey (NHANES), a complex cross-sectional multistage probability sample of the US civilian population. We used data on 5189 eligible adults aged 18 to 85 years. Appropriate survey commands were used and potential confounding variables (including BMI, duration of PPI use, use of other non-PPI medications, and health behaviors) were included in multivariable regression models assessing biomarker outcomes.

Results:
PPI use was associated with differences in mean (±SE) fasting low-density lipoprotein (LDL) (by 11.7±3.7 mg/dL; P=0.006), and apolipoprotein B (by 7.6±2.6 mg/dL; P=0.01). PPI use was not associated with significant differences in total cholesterol (P=0.13), high-density lipoprotein (P=0.27), triglycerides (P=0.70), c-reactive protein (P=0.52), the homeostatic model assessment-insulin resistance (P=0.48), hemoglobin A1c (P=0.39), or homocysteine (P=0.87). PPI use was associated with a decrease in blood urea nitrogen (by 1.0±0.3 mg/dL; P=0.008) but not creatinine (P=0.38) or uric acid (P=0.34).

Conclusion:
PPI was not associated with clinically significant differences in serum biomarkers of inflammation, insulin resistance, cardiovascular risk, and renal function. Rather, increasing BMI was strongly associated with PPI use and clinically significant differences in these biomarkers.