bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pseudohoeflea coraliihabitans sp. nov., a poly-β-hydroxybutyrate-producing, halotolerant bacterium isolated from coral sediment in the Dapeng peninsula (Guangdong, China)
Authors: Yu Sheng-yang , Lu Xiao-juan , Zhang Meng-jun , Luan Sheng-ji , Huang Yi , Zheng Wei-shuang 
Published: September 2024

A Gram-stain-negative, strictly aerobic, motile, flagellated, rod-shaped, halotolerant, and poly-β-hydroxyalkanoate-producing bacterium, designated DP4N28-3T, was isolated from offshore sediment surrounding hard coral in the Dapeng peninsula (Guangdong, PR China). Growth occurred at 15–35 °C (optimal at 30 °C), pH 6.0–9.5 (optimal at 6.0–7.0), and 0.0–30.0 % NaCl concentration (w/v, optimal at 0.0–2.0 %), showing halotolerance. Phylogeny based on 16S rRNA gene sequences, five housekeeping genes, and genome sequences identified Pseudohoeflea suaedae DSM 23348T (98.1 %, 16S rRNA gene sequence similarity) as the most related species to strain DP4N28-3T. Average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values between strain DP4N28-3T and P. suaedae DSM 23348T were all below the threshold of species demarcation. Major phenotypic differences were the flagella type and the limited sources of single carbon utilization by strain DP4N28-3T, which only included acetic acid, acetoacetic acid, d-glucuronic acid, and glucuronamide. Strain DP4N28-3T harboured the class I poly-β-hydroxyalkanoate synthase gene (phaC) and produced poly-β-hydroxybutyrate. The fatty acids were summed feature 8 (C18 : 1
ω6c and/or C18 : 1
ω7c, 49.4 %) and C16 : 0 (13.4 %). The major cellular polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, and sulfoquinovosyl diacylglycerol. The respiratory quinone was Q-10. The results of the phylogenetic, genomic, phenotypic, and chemotaxonomic analysis indicated that the isolated strain represents the type strain of a novel species. Based on these results, strain DP4N28-3T (=MCCC 1K05639T=KCTC 82803T) is proposed as the type strain of the novel species Pseudohoeflea coraliihabitans sp. nov.
Polaribacter ponticola sp. nov., isolated from seawater, reclassification of Polaribacter undariae as a later heterotypic synonym of Polaribacter sejongensis, and emended description of Polaribacter sejongensis Kim et al. 2013
Authors: Baek Ju Hye , Butt Mahrukh , Han Dong Min , Kim Jeong Min , Choi Seohui , Jeon Che Ok 
Published: September 2024

A Gram-stain-negative, yellow-pigmented, and strictly aerobic bacterium, designated as strain MSW5T, was isolated from seawater of the Yellow Sea in South Korea. The cells were non-motile rods exhibiting oxidase- and catalase-positive activities. Growth was observed at 15–25 °C (optimum, 25 °C) and pH 5.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 1.0–5.0% (w/v) NaCl (optimum, 2.0%). Menaquinone-6 was the sole respiratory quinone, and iso-C15 : 0, summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), iso-C15 : 0 3-OH, and C15 : 1
ω6c were the major cellular fatty acids. Major polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences and 92 concatenated core protein sequences revealed that strain MSW5T formed a distinct lineage within the genus Polaribacter. The genome of strain MSW5T was 3582 kb in size with a 29.1 mol% G+C content. Strain MSW5T exhibited the highest similarity to Polaribacter atrinae WP25T, with a 97.9% 16S rRNA gene sequence similarity. However, the average nucleotide identity and digital DNA–DNA hybridization values were 79.4 and 23.3%, respectively, indicating that strain MSW5T represents a novel species. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain MSW5T is proposed to represent a novel species, with the name Polaribacter ponticola sp. nov. The type strain is MSW5T (=KACC 22340T=NBRC 116025T). In addition, whole genome sequence comparisons and phenotypic features suggested that Polaribacter sejongensis and Polaribacter undariae belong to the same species, with P. undariae proposed as a later heterotypic synonym of P. sejongensis. An emended description of Polaribacter sejongensis is also proposed.
Modulatory Effects of M3 Muscarinic Acetylcholine Receptor on Inflammatory Profiles of Human Memory T Helper Cells
Authors: Gholizadeh Fatemeh , Hajiaghayi Mehri , Choi Jennifer S. , Little Samuel R. , Rahbari Niloufar , Brotto Kelly , Han Eric , Shih Steve C.C. , Darlington Peter J. 
Published: September 2024

AbstractMemory T helper (Th) cells, generated after immunogenic challenge, are crucial in directing the adaptive immune response. Muscarinic ACh receptor (mAChR) subtypes expressed by immune cells can be stimulated with acetylcholine or muscarinic-selective drug oxotremorine-M. Cholinergic signaling can influence immune cells, but it is not known how cholinergic stimuli regulate memory Th cells. This study focused on the role of mAChRs, specifically the M3 muscarinic ACh receptor (M3R), in the cytokine profile and NF-κB p65 activity of primary human memory Th cells.Memory Th cells (CD3+CD4+CD45RA-CD45RO+) were isolated from healthy participants’ peripheral blood. Cell culture was performed with anti-CD3/anti-CD28/anti-CD2 reagent, oxotremorine-M (M1R-M5R agonist), atropine (M1R-M5R antagonist), and J104129 (M3R-selective antagonist). MR1-MR5 genesCHRM1-CHRM5were measured with RT-qPCR. Protein expression of M3R and phosphorylated NF-κB p65 were quantified by Western blot. The secretion of IFN-γ, IL-17A, and IL-4 was assessed by ELISA and intracellular cytokine staining flow cytometry.CHRM3, encoding M3R, was knocked out using CRISPR-Cas9 gene targeting.Memory Th cells expressed all five mAChR subtypes. Oxotremorine-M increased IFN-γ and IL-17A while reducing IL-4 in an atropine-sensitive manner. Stimulation of mAChRs in cells withCHRM3-knockout or M3R blockade prevented increases in IFN-γ and IL-17A but continued to inhibit IL-4. mAChR stimulation enhanced NF-κB p65 activity without affecting cell proliferation, viability, or M3R expression.This investigation demonstrates that muscarinic signaling increases the pro-inflammatory profile of memory Th cells, including NF-κB p65, IFN-γ, and IL-17A, with a reduction in IL-4. Focusing on M3R blockers could modulate adaptive immune responses and alleviate immune-related conditions.
Effect of education on crop insurance knowledge: evidence from a RCT in China
Authors: Yan Feier , Yi Fujin , Chen Huang 
Published: September 2024

PurposeThis study investigates the effect of education on crop insurance knowledge within the context of noncompliance experiences. In addition, the study delves into the role of government endorsement in education, which is instructive for the implementation of future insurance promotions.Design/methodology/approachThe study designs a randomized controlled trial (RCT) conducted in Jiangsu Province, China. A total of 518 sample farmers were randomly assigned to two experiments: The Education Experiment and the government’s Endorsement Experiment, respectively. After conducting a set of rigorous exogeneity tests, econometric analysis was conducted using baseline survey data and post experiment data.FindingsOur results revealed that insurance education served as an effective tool in improving farmers’ insurance knowledge, especially their understanding of insurance mechanisms. However, this effect can be mitigated by the noncompliant insurance experience of farmers. Moreover, government-endorsed education proved to be more efficient in improving farmers’ insurance knowledge, thus highlighting the significance of building trust between insureds and insurers.Originality/valueThis study contributes to the literature by demonstrating that using a simple education tool, such as, brochures, can effectively improve farmers insurance knowledge. In addition, insurance mechanisms are now more urgently in need of universalization than policy information. Furthermore, by conducting the RCT, this study obtains unbiased causal inference on the effect of education on insurance knowledge and underscores the role of government endorsement in this process. In addition, the study illustrates the tradeoff between insurers’ efforts in enhancing education and regulating noncompliant insurance misconducts, which compromises education efforts. Overall, this study provides insights into the marketing strategies of insurers and government propaganda aimed at stimulating farmers’ incentives to purchase insurance.
Green supply chain management in Thai service organizations
Authors: Bandoophanit Thianthip 
Published: September 2024

Purpose
This study aims to critically examine the implementation of green supply chain management (GSCM) practices in service organizations in Thailand.

Design/methodology/approach
This study observed 17 service organizations in the private, government, state and non-profit sectors. The multiple case study method was used, including semi-structured interviews, observations and documentation. The data were analyzed using content and thematic analysis methods. Abductive reasoning was used to explain new findings that do not align with prior theories.

Findings
A total of 13 of the 17 cases studied had environmental management systems (EMS). Four were GSCM-proactive and moving toward sustainable sufficiency by involving nearby communities in their service delivery. Although regulations and policies from their head offices were key pressures, leaders with high commitment and eco-awareness achieved outstanding success. Instances of greenwashing and fraud were evident among four non-adopters, one of whom was an environmental regulator.

Research limitations/implications
This study acknowledges that leader commitment and high eco-awareness are the most powerful factors. However, the limited timeframe did not allow a deeper exploration of how to create a socially responsible leader. While the openness of information was evident from the best practitioners, non-adopters did not share any eco-certification or reports. They did not allow the researcher to contact other potential respondents apart from the arranged interview sessions. However, correct data were obtained when the answers were contradictory and one(s) told the truth.

Practical implications
A sustainable and sufficient service supply chain model and new equation were proposed, embracing stakeholders such as society, regulators and employees. Recommended practical strategies include green procurement, reduced utility use and reverse logistics (3Rs and 5Ss).

Originality/value
In the GSCM theory, while the manufacturing sector focuses on economics and the environment, green practices in the service sector address social concerns. This study establishes connections between four concepts: EMS-founded GSCM, leading to repeated use of materials in a circular economy (CE). CE is thus the pathway to reducing consumption and achieving real happiness through a sufficiency economy philosophy (SEP).
Empowering waqf (Islamic endowment) entrepreneurs: a study of the key motivational factors underlying waqf-based entrepreneurship in Indonesia
Authors: Maulina Rindawati , Dhewanto Wawan , Faturohman Taufik , Nur Azis Imam 
Published: September 2024

Purpose
This study aims to examine the understudied topic of Islamic entrepreneurship by adapting the framework of waqf-based entrepreneurship to identify the crucial factors driving waqf-based entrepreneurs’ motivation.

Design/methodology/approach
A partial least squares structural equation model was used to validate the hypotheses, and in-depth interviews were conducted to gain deeper insights into the findings.

Findings
The key findings revealed that self-commitment, entrepreneurial skills and family support exert a significant and positive influence on waqfpreneurs’ motivation to engage in waqf-based entrepreneurship, with self-commitment playing a mediating role between self-actualisation and waqfpreneurial motivation. Additionally, the study proposes categorisations of waqfpreneurs based on the volume of waqf funds managed; their capacity to execute waqf-based entrepreneurship; and institutional performance metrics.

Research limitations/implications
The limited sample size restricts the generalisability of the study. Future research should use a larger sample to generate more broadly applicable results. In addition, the methodology can be replicated in other countries to identify new factors influencing waqfpreneurs’ motivation or to uncover aspects not explored in this research. Future studies could also use qualitative methodologies such as grounded theory, ethnography or case studies. This would allow for a deeper exploration of motivational factors not yet captured, directly from the lived experiences of waqfpreneurs/nazhirs.

Practical implications
The study provides evidence of the key factors that influence waqf-based entrepreneurship, contributing to the literature on entrepreneurship and waqf. It also offers valuable insights for the government and other stakeholders into strategies to motivate waqf-based entrepreneurs, ultimately fostering the development of waqf assets, value creation and sustainable waqf benefit distribution.

Originality/value
The study aims to address the lack of research on Islamic entrepreneurship by specifically modifying the framework of waqf-based entrepreneurship to investigate the key factors that influence individuals’ motivation to conduct waqf-based entrepreneurship and management activities.
“Happyfly” – an online intervention to improve the art-of-living and well-being among flight attendants
Authors: Schwarz Mira , Müller Lara Greta , Schmitz Bernhard 
Published: September 2024

PurposeIt is inherent in human nature to pursue a fulfilling life. The art-of-living approach provides strategies to help individuals attain higher well-being. Based on current research approaches on the art-of-living, we aimed to develop, implement and evaluate an online training that enhances art-of-living and well-being scores of flight attendants.Design/methodology/approachThe training focused on six art-of-living components – self-knowledge, savoring, bodily care, coping with events, positive attitude toward life and serenity. In total, 94 participants were randomly assigned to 3-day (n = 34) or 9-day (n = 30) training groups or to 2 corresponding control groups (CGs) (n = 30). Art-of-living and well-being were measured using self-reported questionnaires at pre-intervention, post-intervention and two-week follow-up.FindingsResults showed significant pre-post differences in art-of-living and well-being scores in both experimental groups, while scores for the CGs remained stable across assessments. Intervention effects were sustained over the two-week follow-up period. We found no significant differences in efficacy between the shorter and longer training, suggesting that brief training can be effective.Practical implicationsThese results demonstrate that well-being can be enhanced through online art-of-living training, which is promising in terms of the practical implementation of such training in resource-constrained work environments.Originality/valueThe presented, conducted and evaluated work intervention represents the first study to apply the multi-component approach of “art-of-living” in an online setting, comparing two trainings of varying durations. This approach offers a framework perfectly suited for future implementation in flight attendants’ work settings to increase well-being and a possible subsequent implementation in other professional groups that would benefit from online training (e.g. in a hybrid work context).
Improvisation and strategy: lessons learned from supporting young digital makers in a fully online makerspace club for collaborative game design
Authors: Xia Yu , Yan Shulong , Jiang Mengying 
Published: September 2024

Purpose
The rapid emergency remote teaching has moved informal learning programs online including makerspaces, which has created an opportunity for harnessing the benefits of virtual makerspaces. This study reports on findings from three club designers and facilitators’ reflections on a fully virtual maker club offered in the summer of 2023. This paper aims to inform practitioners of the potential challenges and strategies to resolve various issues.

Design/methodology/approach
Reflective video journaling was conducted by facilitators. Videos were transcribed and analyzed for observations, learning events, challenges and strategies.

Findings
Findings present strategies adopted to address problems during implementation and a checklist for practitioners. The problems are associated with the learning environments, technology tools and student participation. The authors adopted in-the-moment and short-term strategies to tackle the problems and long-term strategies that bear implications for future virtual makerspaces.

Practical implications
This paper can provide direct guidance to practitioners in schools, libraries, museums, etc., who plan to offer informal collaborative learning for children online. It prepares practitioners for the potential challenges and equip them with concrete strategies and questions.

Social implications
Fully virtual makerspaces have the potential to reach a wider range of audience.

Originality/value
To the best of the authors’ knowledge, this paper is the first to report the reflective findings on a fully virtual makerspace club that comprehensively covers challenges and strategies before, during and after the club, which has direct implications for future offerings of such programs.
A visit to the past through historic cafés and botequins: a comparison between Lisbon and Rio de Janeiro
Authors: Palrão Teresa , Madeira Arlindo , Lavandoski Joice , Rodrigues Rosa , Lopes Sofia 
Published: September 2024

PurposeExplore the role of traditional cafés in Lisbon and botequins in Rio de Janeiro in the history and culture of their respective cities, based on an analysis of the comments published on TripAdvisor.Design/methodology/approachUsing a mixed methodology, 7,430 evaluations were analyzed, of which 5,473 were for Portuguese cafés and 1,957 for Brazilian botequins. A content analysis and statistical treatment were carried out. A line-by-line analysis was also conducted to code the emotions reported in the customer reviews.FindingsThe analysis categorized the comments into Gastronomy, Nostalgia, History and Heritage, and Quality of Service. Nostalgia garnered the highest percentage of positive comments for both Portuguese cafés and Brazilian botequins. These results emphasize the importance attached to sentimental connections and historical narratives, particularly those associated with notable individuals who frequented these places. Furthermore, no statistically significant differences were identified according to the type of establishment, which suggests a consistent pattern between cafés and traditional botequins.Practical implicationsDraws attention to the importance of a collaborative partnership between restaurant owners and tourism authorities. This strategic alliance not only serves to raise the cultural profile of cities, but also generates substantial economic benefits. It also ensures the lasting legacy of these historic establishments, celebrating the past, enriching the present and safeguarding the future.Originality/valueStudying the cafés of Lisbon and the botequins of Brazil is important not only for understanding the history and culture of these countries but also for analyzing issues related to socialization, identity, artistic expression and urban transformations.
Income inequality, unemployment, and government transfer: what do their dynamics tell us
Authors: Ahmed Haydory Akbar , Shadmani Hedieh 
Published: September 2024

PurposeIn this research, we explore the dynamics among measures of income inequality in the USA, male and female unemployment rates and growth in government transfer using time series data.Design/methodology/approachThis research adopts a macro-econometric approach to estimate a structural VAR model using time series data.FindingsOur structural impulse responses found that growth in government transfer increases unemployment rates for both males and females. Female income inequality declines with increased government transfer. When the female income ratio rises, we observe that government transfer outlays fall over the forecast horizon. Variance decomposition finds that growth in government transfers is impacted by the male unemployment rate relatively more than the female unemployment rate. This research, therefore, suggests gender-specific government transfers to reduce income inequality. This, in effect, may reduce government transfer outlays over time.Practical implicationsThis research, therefore, suggests gender-specific government transfers to reduce income inequality. This, in effect, may reduce government transfer outlays over time.Originality/valueThis research investigates the dynamics among income inequality, government transfer and unemployment rates. There is a dearth of research articles that adopt a macro-econometric in this area.
Quality of care transitions from hospital to home for COVID-19 patients discharged from Brazilian university hospitals
Authors: Wachholz Laísa Fischer , Cechinel-Peiter Caroline , Costa Maria Fernanda Baeta Neves Alonso da , Acosta Aline Marques , Erdmann Alacoque Lorenzini , Santos José Luis Guedes dos , Backman Chantal , Lanzoni Gabriela Marcellino de Melo 
Published: September 2024

PurposeTo analyze the quality of transitional care for patients with COVID-19 at discharge from Brazilian university hospitals.Design/methodology/approachA cross-sectional descriptive study was carried out in five Brazilian university hospitals between April and December 2021. The sample consisted of 527 participants. Data collection consisted of a sociodemographic questionnaire and the Care Transitions Measure (CTM-15), a care transition assessment instrument, which was translated and validated in Portuguese.FindingsMost participants were patients (n = 369; 70.0%), with primary school completion (n = 218; 43.4%), multiracial (n = 218; 43.5%) and with an income of up to two minimum wages (n = 182; 42.8%). Dimension 1 – management preparation – obtained the highest score (71.2 points, SD = 16.5), while Dimension 4 – care plan – obtained the lowest score (62.2 points, SD = 23.4). Among the participating hospitals, there was a difference in the overall mean with results ranging from 67.0 to 72.9 points.Originality/valueA satisfactory quality of care transition was found, considering the context of a pandemic. The main weaknesses in the care transitions were related to the care planning after hospital discharge.