bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of
Paecilomyces hepiali
, Isolated from
Cordyceps sinensis
Authors: Yu Yi , Wang Wenting , Wang Linping , Pang Fang , Guo Lanping , Song Lai , Liu Guiming , Feng Chengqiang 
Published: July 2016

ABSTRACT

Paecilomyces hepiali
is an endoparasitic fungus that commonly exists in the natural
Cordyceps sinensis
. Here, we report the draft genome sequence of
P. hepiali
, which will facilitate the exploitation of medicinal compounds produced by the fungus.
Complete Genome Sequence of Porcine Epidemic Diarrhea Virus from an Outbreak in a Vaccinated Farm in Shandong, China
Authors: Gao Xiang , Li Dongliang , Zhao Jingyi , Xu Farong , Ge Xinna , Guo Xin , Han Jun , Yang Hanchun , Zhou Lei 
Published: July 2016

ABSTRACT

Porcine epidemic diarrhea virus, a member of the family
Coronaviridae
, is an economically important pathogen that causes severe enteritis, vomiting, dehydration, and a high mortality rate, especially among suckling piglets. Here, we report the complete genome sequence (28,036 nucleotides [nt]) of a porcine epidemic diarrhea virus (PEDV) strain isolated in a novel outbreak in Shandong, China.
Draft Genome Sequence of
Burkholderia
sp. Strain CCA53, Isolated from Leaf Soil
Authors: Akita Hironaga , Kimura Zen-ichiro , Yusoff Mohd Zulkhairi Mohd , Nakashima Nobutaka , Hoshino Tamotsu 
Published: July 2016

ABSTRACT

Burkholderia
sp. strain CCA53 was isolated from leaf soil collected in Higashi-Hiroshima City in Hiroshima Prefecture, Japan. Here, we present a draft genome sequence of this strain, which consists of a total of 4 contigs containing 6,647,893 bp, with a G+C content of 67.0% and comprising 9,329 predicted coding sequences.
Permanent Draft Genome Sequence for
Frankia
sp
.
Strain EI5c, a Single-Spore Isolate of a Nitrogen-Fixing Actinobacterium, Isolated from the Root Nodules of
Elaeagnus angustifolia
Authors: D'Angelo Timothy , Oshone Rediet , Abebe-Akele Feseha , Simpson Stephen , Morris Krystalynne , Thomas W. Kelley , Tisa Louis S. 
Published: July 2016

ABSTRACT

Frankia
sp. strain EI5c is a member of
Frankia
lineage III, which is able to reinfect plants of the
Eleagnaceae
,
Rhamnaceae
,
Myricaceae
, and
Gymnostoma
, as well as the genus
Alnus
. Here, we report the 6.6-Mbp draft genome sequence of
Frankia
sp. strain EI5c with a G+C content of 72.14 % and 5,458 candidate protein-encoding genes.
Draft Genome Sequence for the Type Strain of
Corynebacterium afermentans
LCDC 88-0199
T
, Isolated from a Human Blood Culture
Authors: Bernier Anne-Marie , Bernard Kathryn 
Published: July 2016

ABSTRACT

A draft genome for
Corynebacterium afermentans
LCDC 88-0199
T
was investigated. The size of the genome was 2,345,615 bp with an observed G+C content of 64.85%. Annotation revealed 2 rRNA sequences, 54 tRNA genes, and 2,164 coding sequences. Genome coverage was 85× and consisted of 24 contigs with an
N
50
of 187,988 bp.
What can we learn on public accountability from non-health disciplines: a meta-narrative review
Authors: Van Belle Sara , Mayhew Susannah H 
Published: July 2016

ObjectiveIn health, accountability has since long been acknowledged as a central issue, but it remains an elusive concept. The literature on accountability spans various disciplines and research traditions, with differing interpretations. There has been little transfer of ideas and concepts from other disciplines to public health and global health. In the frame of a study of accountability of (international) non-governmental organisations in local health systems, we carried out a meta-narrative review to address this gap. Our research questions were: (1) What are the main approaches to accountability in the selected research traditions? (2) How is accountability defined? (3) Which current accountability approaches are relevant for the organisation and regulation of local health systems and its multiple actors?SettingThe search covered peer-reviewed journals, monographs and readers published between 1992 and 2012 from political science, public administration, organisational sociology, ethics and development studies. 34 papers were selected and analysed.ResultsOur review confirms the wide range of approaches to the conceptualisation of accountability. The definition of accountability used by the authors allows the categorisation of these approaches into four groups: the institutionalist, rights-based, individual choice and collective action group. These four approaches can be considered to be complementary.ConclusionsWe argue that in order to effectively achieve public accountability, accountability strategies are to be complementary and synergistic.
Review of 99 self-report measures for assessing well-being in adults: exploring dimensions of well-being and developments over time
Authors: Linton Myles-Jay , Dieppe Paul , Medina-Lara Antonieta 
Published: July 2016

ObjectiveInvestigators within many disciplines are using measures of well-being, but it is not always clear what they are measuring, or which instruments may best meet their objectives. The aims of this review were to: systematically identify well-being instruments, explore the variety of well-being dimensions within instruments and describe how the production of instruments has developed over time.DesignSystematic searches, thematic analysis and narrative synthesis were undertaken.Data sourcesMEDLINE, EMBASE, EconLit, PsycINFO, Cochrane Library and CINAHL from 1993 to 2014 complemented by web searches and expert consultations through 2015.Eligibility criteriaInstruments were selected for review if they were designed for adults (≥18 years old), generic (ie, non-disease or context specific) and available in an English version.ResultsA total of 99 measures of well-being were included, and 196 dimensions of well-being were identified within them. Dimensions clustered around 6 key thematic domains: mental well-being, social well-being, physical well-being, spiritual well-being, activities and functioning, and personal circumstances. Authors were rarely explicit about how existing theories had influenced the design of their tools; however, the 2 most referenced theories were Diener's model of subjective well-being and the WHO definition of health. The period between 1990 and 1999 produced the greatest number of newly developed well-being instruments (n=27). An illustration of the dimensions identified and the instruments that measure them is provided within a thematic framework of well-being.ConclusionsThis review provides researchers with an organised toolkit of instruments, dimensions and an accompanying glossary. The striking variability between instruments supports the need to pay close attention to what is being assessed under the umbrella of ‘well-being’ measurement.
Association of common mental disorder symptoms with health and healthcare factors among women in rural western India: results of a cross-sectional survey
Authors: Soni Apurv , Fahey Nisha , Byatt Nancy , Prabhakaran Anusha , Moore Simas Tiffany A , Vankar Jagdish , Phatak Ajay , O'Keefe Eileen , Allison Jeroan , Nimbalkar Somashekhar 
Published: July 2016

ObjectivesInformation about common mental disorders (CMD) is needed to guide policy and clinical interventions in low-income and middle-income countries. This study's purpose was to characterise the association of CMD symptoms with 3 inter-related health and healthcare factors among women from rural western India based on a representative, cross-sectional survey.SettingSurveys were conducted in the waiting area of various outpatient clinics at a tertiary care hospital and in 16 rural villages in the Anand district of Gujarat, India.Participants700 Gujarati-speaking women between the ages of 18–45 years who resided in the Anand district of Gujarat, India, were recruited in a quasi-randomised manner.Primary and secondary outcomes measuresCMD symptoms, ascertained using WHO's Self-Reporting Questionnaire-20 (SRQ-20), were associated with self-reported (1) number of healthcare visits in the prior year; (2) health status and (3) portion of yearly income expended on healthcare.ResultsData from 658 participants were used in this analysis; 19 surveys were excluded due to incompleteness, 18 surveys were excluded because the participants were visiting hospitalised patients and 5 surveys were classified as outliers. Overall, 155 (22·8%) participants screened positive for CMD symptoms (SRQ-20 score ≥8) with most (81.9%) not previously diagnosed despite contact with healthcare provider in the prior year. On adjusted analyses, screening positive for CMD symptoms was associated with worse category in self-reported health status (cumulative OR=9.39; 95% CI 5·97 to 14·76), higher portion of household income expended on healthcare (cumulative OR=2·31; 95% CL 1·52 to 3.52) and increased healthcare visits in the prior year (incidence rate ratio=1·24; 95% CI 1·07 to 1·44).ConclusionsThe high prevalence of potential CMD among women in rural India that is unrecognised and associated with adverse health and financial indicators highlights the individual and public health burden of CMD.
Prospective assessment of neurodevelopment in children following a pregnancy complicated by severe pre-eclampsia
Authors: Warshafsky Chelsie , Pudwell Jessica , Walker Mark , Wen Shi-Wu , Smith Graeme N 
Published: July 2016

ObjectiveTo prospectively examine whether children of women with a pregnancy affected by severe pre-eclampsia (PE), compared to children of women without a PE-affected pregnancy, have differences in neurodevelopmental performance up to 5 years of age.DesignProspective cohort study.SettingTertiary care centre.ParticipantsWomen were recruited following a PE-affected pregnancy. After each PE participant was recruited, the next normotensive woman without a prior history of PE and matched by parity, maternal age and race was invited to participate. Women with a history of chronic hypertension, diabetes or renal disease were excluded. Total enrolment included 129 PE-affected and 140 normotensive mothers.Outcome measuresThe primary outcome measure was failure of the Ages and Stages Questionnaire (ASQ). The ASQ was completed yearly, until age 5.ResultsA significant difference was found in the proportion of ASQ categories failed in year 3 (p<0.05), and this approached significance in years 1 and 4 (p<0.10 and p<0.15, respectively). At year 1, the number of ASQ categories failed was significantly greater among children born to PE mothers. A subgroup analysis revealed that a significant proportion of PE children born preterm (<37 weeks) failed the ASQ in years 3 and 4 (p<0.05), and when failed, those who were preterm failed significantly more categories (p<0.05). A trend towards increased failure in the gross motor category was found. There was a significant positive correlation between maternal lifetime CVD risk score and number of ASQ categories failed at years 1 and 3 (p<0.05).ConclusionsSevere PE is associated with other adverse pregnancy outcomes, including intrauterine growth restriction and preterm birth, all of which are associated with increased neurodevelopment delays. Thus, PE indicates a need for early screening and intervention at the neurodevelopmental level to improve children's long-term health, with larger studies required to tease out contributing factors.
Low-glycaemic index diet to improve glycaemic control and cardiovascular disease in type 2 diabetes: design and methods for a randomised, controlled, clinical trial
Authors: Chiavaroli Laura , Mirrahimi Arash , Ireland Christopher , Mitchell Sandra , Sahye-Pudaruth Sandhya , Coveney Judy , Olowoyeye Omodele , Maraj Tishan , Patel Darshna , de Souza Russell J , Augustin Livia S A , Bashyam Balachandran , Blanco Mejia Sonia , Nishi Stephanie K , Leiter Lawrence A , Josse Robert G , McKeown-Eyssen Gail , Moody Alan R , Berger Alan R , Kendall Cyril W C , Sievenpiper John L , Jenkins David J A 
Published: July 2016

IntroductionType 2 diabetes (T2DM) produces macrovascular and microvascular damage, significantly increasing the risk of cardiovascular disease (CVD), renal failure and blindness. As rates of T2DM rise, the need for effective dietary and other lifestyle changes to improve diabetes management become more urgent. Low-glycaemic index (GI) diets may improve glycaemic control in diabetes in the short term; however, there is a lack of evidence on the long-term adherence to low-GI diets, as well as on the association with surrogate markers of CVD beyond traditional risk factors. Recently, advances have been made in measures of subclinical arterial disease through the use of MRI, which, along with standard measures from carotid ultrasound (CUS) scanning, have been associated with CVD events. We therefore designed a randomised, controlled, clinical trial to assess whether low-GI dietary advice can significantly improve surrogate markers of CVD and long-term glycaemic control in T2DM.Methods and analysis169 otherwise healthy individuals with T2DM were recruited to receive intensive counselling on a low-GI or high-cereal fibre diet for 3 years. To assess macrovascular disease, MRI and CUS are used, and to assess microvascular disease, retinal photography and 24-hour urinary collections are taken at baseline and years 1 and 3. Risk factors for CVD are assessed every 3 months.Ethics and disseminationThe study protocol and consent form have been approved by the research ethics board of St. Michael's Hospital. If the study shows a benefit, these data will support the use of low-GI and/or high-fibre foods in the management of T2DM and its complications.Trial Registration numberNCT01063374; Pre-results.
The impact of an inflammatory bowel disease nurse-led biologics service
Authors: Taylor Nicola S , Bettey Marion , Wright Julia , Underhill Caron , Kerr Sarah , Perry Kim , Cummings JR Fraser 
Published: July 2016

IntroductionSouthampton General Hospital provides inflammatory bowel disease (IBD) services for a population of 650 000. Biological agents have impacted hugely on IBD but are costly drugs requiring careful supervision. These challenges led us to develop a specialist nurse-led biologics service to improve patient care.MethodA 2010 case note audit highlighted areas for improvement in monitoring biologics and follow-up. A business case was developed to establish an IBD nurse to ensure identification and appropriate screening, education and review of biologics patients. A gain share was agreed with the local Care Commissioning Group (CCG) and £60 000 invested. Outcomes were reaudited in 2014.ResultsBiologic use has grown rapidly from 90 patients in 2011 to 330 in 2014. All records are now kept in a centralised database. Infection screening improved from 79% to 100%. In 2014, 96% of patients had follow-up ≤4 months post-induction to assess response, but two patients were seen at 7 months. 80% were followed up again at 9–12 months (100% at 9–14 months), all with treatment decisions. The initial investment was recouped via commissioners funding 368 additional outpatient appointments and 35 colonoscopies. Savings represented 15% total yearly biologic costs.ConclusionsThe introduction of the IBD biologics nurse-led service resulted in significant gains in care quality and costs. The need for improved follow-up of patients on biologics reflects increased pressures on clinic resources across the country. With continued biologics expansion, the introduction of a biologics nurse has provided invaluable support to patients and the IBD team at Southampton General Hospital.
Flow changes in the posterior communicating artery related to flow-diverter stents in carotid siphon aneurysms
Authors: de Carvalho Fernando Mota , Caroff Jildaz , Pereira dos Santos Neto Elizeu , Chalumeau Vanessa , Abdel Khalek Hazem , Neki Hiroaki , Saliou Guillaume , Rouchaud Aymeric , Moret Jacques , Spelle Laurent 
Published: July 2016

BackgroundFlow-diverter stent (FDS) placement for treatment of intracranial aneurysms can cause flow changes in the covered branches.ObjectiveTo assess the impact of the treatment of carotid siphon aneurysms with FDS on the posterior communicating artery (PComA) flow.Materials and methodsBetween February 2011 and January 2015, 125 carotid siphon aneurysms were treated with FDS. We retrospectively analyzed all cases with PComA ostial coverage. The circle of Willis anatomy was also studied as the flow changes in PComA postoperatively and during angiographic follow-up. Data from neurological examination were also collected.ResultsEighteen aneurysms of the carotid siphon in 17 patients were treated with FDS covering the ostium of the PComA. Based on the initial angiography, patients were divided into two groups: the first with a P1/PComA size ratio >1 (10 cases) and the second with a ratio ≤1 (8 cases). Follow-up angiography (mean time of 10 months) showed 90% of PComA flow changes in group 1 but only 12.5% in group 2. There was a significant difference between the two groups (p=0.002). Nevertheless, no patient had new symptoms related to these flow changes during the follow-up period.ConclusionsIn our experience, covering the PComA by FDS when treating carotid siphon aneurysms appeared safe and the P1/PComA ratio is a good predictor of flow changes in PComA.
Combined endovascular coiling and intra-aneurysmal allogeneic mesenchymal stromal cell therapy for intracranial aneurysms in a rabbit model: a proof-of-concept study
Authors: Adibi Amin , Eesa Muneer , Wong John H , Sen Arindom , Mitha Alim P 
Published: July 2016

ObjectiveTo assess the feasibility and efficacy of clinically translatable adjuvant mesenchymal stem/stromal cells (MSCs) therapy in improving the healing of coiled aneurysms in a rabbit elastase aneurysm model.MethodsBone marrow-derived MSC populations were isolated from three rabbit donors in a serum-free environment and independently characterized to confirm their identity. Elastase-induced carotid aneurysms were created in nine New Zealand white rabbits. Each animal received one of the following treatments based on previous randomization: (1) coiling alone (control group); (2) coiling with an intra-aneurysmal injection of saline (vehicle group); and (3) coiling with an intra-aneurysmal injection of 5 million allogeneic MSCs (treatment group). The animals were followed for 4 weeks post-treatment, at the end of which blinded analyses of angiograms and histology were performed.ResultsHistological results in the treatment group showed improvements over the control and vehicle groups, although the improvement over the vehicle group was not significant. Intra-aneurysmal cell therapy with 5 million allogeneic MSCs did not result in any major adverse events. Angiographic results did not show any significant difference among groups.ConclusionsThis proof-of-concept study shows that adjuvant MSC therapy for intracranial aneurysms is feasible and may enhance histological improvement of coiled aneurysms at 4 weeks post-treatment.
Osteoporosis prevention among chronic glucocorticoid users: results from a public health insurance database
Authors: Trijau Sophie , de Lamotte Gaëlle , Pradel Vincent , Natali François , Allaria-Lapierre Véronique , Coudert Hervé , Pham Thao , Sciortino Vincent , Lafforgue Pierre 
Published: July 2016

IntroductionLong-term glucocorticoid therapy is the leading cause of secondary osteoporosis. The management of glucocorticoid-induced osteoporosis (GIOP) seems to be inadequate in many European countries.ObjectiveTo evaluate the rate of screening and treatment of GIOP.DesignInformation was collected from a national public health-insurance database in our geographic area of Provence-Alpes-Côte-d'Azur and in Corsica, from September 2009 through August 2011.PatientsWe identified participants aged 15 years and over starting glucocorticoid therapy (≥7.5 mg of prednisone equivalent per day during at least 90 days consecutive). This cohort was compared with an age-matched and sex-matched population that did not receive glucocorticoids.Main outcome measuresBone mass, prescription of bone antiresorptive medication and use of calcium and/or vitamin D treatment.ResultsWe identified 32 812 patients who were prescribed glucocorticoid therapy, yielding 1% prevalence. Incidence of glucocorticoid therapy was 2.8/1000 inhabitants/year. Males represented 44%, the mean age was 58 years. The median prednisone-equivalent dose was 11 mg/day (IQR 9–18 mg/day). 8% underwent bone mass measurement. Calcium and/or vitamin D, and bisphosphonates were prescribed in 18% and 12%, respectively. Results were lower for the control population: 3% underwent bone mass measurement and 3% received bisphosphonate therapy. The rates of osteodensitometry and treatments were higher in women over 55 years of age than in men and women 55 years of age and younger, and also when glucocorticoid therapy was initiated by a rheumatologist versus other physician specialty.ConclusionsThe management of GIOP remains very inadequate, despite the availability of a statutory health insurance system. Targeted interventions are needed to improve the management of GIOP.
Mutational Landscape and Sensitivity to Immune Checkpoint Blockers
Authors: Chabanon Roman M. , Pedrero Marion , Lefebvre Céline , Marabelle Aurélien , Soria Jean-Charles , Postel-Vinay Sophie 
Published: July 2016

Abstract
Immunotherapy is currently transforming cancer treatment. Notably, immune checkpoint blockers (ICB) have shown unprecedented therapeutic successes in numerous tumor types, including cancers that were traditionally considered as nonimmunogenic. However, a significant proportion of patients do not respond to these therapies. Thus, early selection of the most sensitive patients is key, and the development of predictive companion biomarkers constitutes one of the biggest challenges of ICB development. Recent publications have suggested that the tumor genomic landscape, mutational load, and tumor-specific neoantigens are potential determinants of the response to ICB and can influence patients' outcomes upon immunotherapy. Furthermore, defects in the DNA repair machinery have consistently been associated with improved survival and durable clinical benefit from ICB. Thus, closely reflecting the DNA damage repair capacity of tumor cells and their intrinsic genomic instability, the mutational load and its associated tumor-specific neoantigens appear as key predictive paths to anticipate potential clinical benefits of ICB. In the era of next-generation sequencing, while more and more patients are getting the full molecular portrait of their tumor, it is crucial to optimally exploit sequencing data for the benefit of patients. Therefore, sequencing technologies, analytic tools, and relevant criteria for mutational load and neoantigens prediction should be homogenized and combined in more integrative pipelines to fully optimize the measurement of such parameters, so that these biomarkers can ultimately reach the analytic validity and reproducibility required for a clinical implementation. Clin Cancer Res; 22(17); 4309–21. ©2016 AACR.
Cor e a construção cultural
Authors: Gomide Glória Itabirano 
Published: July 2016

Historicamente, a cor sempre teve um papel de significação ideológica. Logo, cabe neste momento em que manifestações partidárias urgem nas ruas – normalmente em conclames pelas redes sociais – um estudo sobre sua representação. A proposta é, portanto estudar a origem e a psicodinâmica das cores; a cor como representação ideológica; as cores usadas como símbolos de patriotismo ou totalitarismo. Além disso, analisar a construção de um discurso sociocultural através da cor, refletindo também sobre como o azul-verde-amarelo se tornou uma conjunção camaleônica representante da cada manifestação política.Como observação final refletir, a quem pertence as cores da bandeira nacional, se é que elas têm dono.