bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manipulation of photoluminescence of two-dimensional MoSe2 by gold nanoantennas
Authors: Chen Haitao , Yang Jiong , Rusak Evgenia , Straubel Jakob , Guo Rui , Myint Ye Win , Pei Jiajie , Decker Manuel , Staude Isabelle , Rockstuhl Carsten , Lu Yuerui , Kivshar Yuri S. , Neshev Dragomir 
Published: February 2016

AbstractMonolayer molybdenum diselenide (MoSe2), a member of the TMDCs family, is an appealing candidate for coupling to gold plasmonic nanostructures as it has smaller bandgap and higher electron mobility in comparison to frequently studied molybdenum disulfide (MoS2). The PL of MoSe2 occurs in the near-infrared spectral range where the emissive properties do not suffer from the enhanced dissipation in the gold due to inter-band transitions. Here, we study the interaction between monolayer MoSe2 and plasmonic dipolar antennas in resonance with the PL emission of MoSe2. By varying the thickness of the spacer between the MoSe2 layer and nanoantenna, we demonstrate manipulation of the PL intensity from nearly fourfold quenching to approximately threefold enhancement. Furthermore, we show that the coupled TMDC-nanoantenna system exhibits strong polarization-dependent PL, thus offering the possibility of polarization-based emission control. Our experimental results are supported by numerical simulations as well. To the best of our knowledge, this is the first study of Au-MoSe2 plasmonic hybrid structures realizing flexible PL manipulation.
Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy
Authors: Huang Xinwei , Yue Yaofei , Li Duo , Zhao Yujiao , Qiu Lijuan , Chen Junying , Pan Yue , Xi Juemin , Wang Xiaodan , Sun Qiangming , Li Qihan 
Published: February 2016

AbstractAntibody dependent enhancement (ADE) of dengue virus (DENV) infection is identified as the main risk factor of severe Dengue diseases. Through opsonization by subneutralizing or non-neutralizing antibodies, DENV infection suppresses innate cell immunity to facilitate viral replication. However, it is largely unknown whether suppression of type-I IFN is necessary for a successful ADE infection. Here, we report that both DENV and DENV-ADE infection induce an early ISG (NOS2) expression through RLR-MAVS signalling axis independent of the IFNs signaling. Besides, DENV-ADE suppress this early antiviral response through increased autophagy formation rather than induction of IL-10 secretion. The early induced autophagic proteins ATG5-ATG12 participate in suppression of MAVS mediated ISGs induction. Our findings suggest a mechanism for DENV to evade the early antiviral response before IFN signalling activation. Altogether, these results add knowledge about the complexity of ADE infection and contribute further to research on therapeutic strategies.
Ultrafast adiabatic quantum algorithm for the NP-complete exact cover problem
Authors: Wang Hefeng , Wu Lian-Ao 
Published: February 2016

AbstractAn adiabatic quantum algorithm may lose quantumness such as quantum coherence entirely in its long runtime and consequently the expected quantum speedup of the algorithm does not show up. Here we present a general ultrafast adiabatic quantum algorithm. We show that by applying a sequence of fast random or regular signals during evolution, the runtime can be reduced substantially, whereas advantages of the adiabatic algorithm remain intact. We also propose a randomized Trotter formula and show that the driving Hamiltonian and the proposed sequence of fast signals can be implemented simultaneously. We illustrate the algorithm by solving the NP-complete 3-bit exact cover problem (EC3), where NP stands for nondeterministic polynomial time and put forward an approach to implementing the problem with trapped ions.
CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo
Authors: Chang Hong , Yi Bin , Ma Ruixia , Zhang Xiaoguo , Zhao Hongyou , Xi Yaguang 
Published: February 2016

AbstractMicroRNAs are small and non-coding RNA molecules with the master role in regulation of gene expression at post-transcriptional/translational levels. Many methods have been developed for microRNA loss-of-function study, such as antisense inhibitors and sponges; however, the robustness, specificity, and stability of these traditional strategies are not highly satisfied. CRISPR/cas9 system is emerging as a novel genome editing tool in biology/medicine research, but its indication in microRNA research has not been studied exclusively. In this study, we clone CRISPR/cas9 constructs with single-guide RNAs specifically targeting biogenesis processing sites of selected microRNAs; and we find that CRISPR/cas9 can robustly and specifically reduce the expression of these microRNAs up to 96%. CRISPR/cas9 also shows an exclusive benefit in control of crossing off-target effect on microRNAs in the same family or with highly conserved sequences. More significantly, for the first time, we demonstrate the long term stability of microRNA knockdown phenotype by CRISPR/cas9 in both in vitro and in vivo models.
Quantitative map of multiple auditory cortical regions with a stereotaxic fine-scale atlas of the mouse brain
Authors: Tsukano Hiroaki , Horie Masao , Hishida Ryuichi , Takahashi Kuniyuki , Takebayashi Hirohide , Shibuki Katsuei 
Published: February 2016

AbstractOptical imaging studies have recently revealed the presence of multiple auditory cortical regions in the mouse brain. We have previously demonstrated, using flavoprotein fluorescence imaging, at least six regions in the mouse auditory cortex, including the anterior auditory field (AAF), primary auditory cortex (AI), the secondary auditory field (AII), dorsoanterior field (DA), dorsomedial field (DM) and dorsoposterior field (DP). While multiple regions in the visual cortex and somatosensory cortex have been annotated and consolidated in recent brain atlases, the multiple auditory cortical regions have not yet been presented from a coronal view. In the current study, we obtained regional coordinates of the six auditory cortical regions of the C57BL/6 mouse brain and illustrated these regions on template coronal brain slices. These results should reinforce the existing mouse brain atlases and support future studies in the auditory cortex.
Ionic Origin of Electro-osmotic Flow Hysteresis
Authors: Lim Chun Yee , Lim An Eng , Lam Yee Cheong 
Published: February 2016

AbstractElectro-osmotic flow, the driving of fluid at nano- or micro- scales with electric field, has found numerous applications, ranging from pumping to chemical and biomedical analyses in micro-devices. Electro-osmotic flow exhibits a puzzling hysteretic behavior when two fluids with different concentrations displace one another. The flow rate is faster when a higher concentration solution displaces a lower concentration one as compared to the flow in the reverse direction. Although electro-osmotic flow is a surface phenomenon, rather counter intuitively we demonstrate that electro-osmotic flow hysteresis originates from the accumulation or depletion of pH-governing minority ions in the bulk of the fluid, due to the imbalance of electric-field-induced ion flux. The pH and flow velocity are changed, depending on the flow direction. The understanding of electro-osmotic flow hysteresis is critical for accurate fluid flow control in microfluidic devices and maintaining of constant pH in chemical and biological systems under an electric field.
miRepress: modelling gene expression regulation by microRNA with non-conventional binding sites
Authors: Ghosal Suman , Saha Shekhar , Das Shaoli , Sen Rituparno , Goswami Swagata , Jana Siddhartha S. , Chakrabarti Jayprokas 
Published: February 2016

AbstractSome earlier studies have reported an alternative mode of microRNA-target interaction. We detected target regions within mRNA transcripts from AGO PAR-CLIP that did not contain any conventional microRNA seed pairing but only had non-conventional binding sites with microRNA 3′ end. Our study from 7 set of data that measured global protein fold change after microRNA transfection pointed towards the association of target protein fold change with 6-mer and 7-mer target sites involving microRNA 3′ end. We developed a model to predict the degree of microRNA target regulation in terms of protein fold changes from the number of different conventional and non-conventional target sites present in the target, and found significant correlation of its output with protein expression changes. We validated the effect of non-conventional interactions with target by modulating the abundance of microRNA in a human breast cancer cell line MCF-7. The validation was done using luciferase assay and immunoblot analysis for our predicted non-conventional microRNA-target pair WNT1 (3′ UTR) and miR-367-5p and immunoblot analysis for another predicted non-conventional microRNA-target pair MYH10 (coding region) and miR-181a-5p. Both experiments showed inhibition of targets by transfection of microRNA mimics that were predicted to have only non-conventional sites.
Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging
Authors: Perkins R. G. , Williamson C. J. , Brodie J. , Barillé L. , Launeau P. , Lavaud J. , Yallop M. L. , Jesus B. 
Published: February 2016

AbstractCalcifying coralline macroalgae provide biogenic habitats colonised by epiphytic microalgae that contribute significantly to community productivity. Georeferenced hyperspectral and high-resolution fluorescence imaging were coupled to microspatially mapped community composition and relative biomass of macroalgal host and epiphyte microalgal groups and their weighted contributions to productivity within host fronds of Corallina officinalis on upper and lower zones of a rocky shore were determined. Lower shore epiphytes were dominated by filamentous diatoms (Bacillariophyta), confined to the apex of the frond structure, which were low light acclimated but retained a high capacity for photoprotective down regulation and contributed up to 51% of total community productivity. Upper shore epiphytes were dominated by green algae (Chlorophyta) and single-celled diatoms (principally Cocconeis spp.), which were high light acclimated but present at far lower relative biomass and contributed negligibly to productivity. The host, C. officinalis was the main primary producer. Variation in light environment resulting from differences in shore height and shading within the host macroalga, likely play a large role in determining patterns in epiphyte community structure, biomass and productivity observed. Additionally, microspatial gradients in photophysiological parameters along the host macroalga likely resulted from age-dependent variation in pigments as well as the gradient in light environment.
Highly divergent dengue virus type 1 genotype sets a new distance record
Authors: Pyke Alyssa T. , Moore Peter R. , Taylor Carmel T. , Hall-Mendelin Sonja , Cameron Jane N. , Hewitson Glen R. , Pukallus Dennis S. , Huang Bixing , Warrilow David , van den Hurk Andrew F. 
Published: February 2016

AbstractDengue viruses (DENVs) are the leading cause of mosquito-borne viral disease of humans. They exist in both endemic and sylvatic ecotypes. In 2014, a viremic patient who had recently visited the rainforests of Brunei returned to Australia displaying symptoms consistent with DENV infection. A unique DENV strain was subsequently isolated from the patient, which we propose belongs to a new genotype within DENV serotype 1 (DENV-1). Bayesian evolutionary phylogenetic analysis suggests that the putative sylvatic DENV-1 Brunei 2014 (Brun2014) is the most divergent DENV-1 yet recorded and increases the time to the most recent common ancestor (MRCA) for DENV-1 from ≈120 years to ≈315 years. DENV-1 classification of the Brun2014 strain was further supported by monoclonal antibody serotyping data. Phenotypic characterization demonstrated that Brun2014 replication rates in mosquito cells and infection rates in Aedes aegypti mosquitoes were not significantly different from an epidemic DENV-1 strain. Given its ability to cause human illness and infect Ae. aegypti, potential urban spillover and clinical disease from further Brun2014 transmission cannot be discounted.
Miniaturised master‐oscillator power‐amplifier emitting a single longitudinal mode with more than 300 mW at 647 nm
Authors: Blume Gunnar , Pohl Johannes , Feise David , Wiedmann Jörg , Matalla Matthias , John Wilfred , Ressel Peter , Kreuzmann Sabrina , Ginolas Arnim , Sahm Alexander , Johne Pia , Jendrzejewski Maik , Kürbis Christian , Hofmann Julian , Eppich Bernd , Erbert Götz , Paschke Katrin 
Published: February 2016

A miniaturised red‐emitting master‐oscillator power‐amplifier (MOPA) was developed. The MOPA emits a single longitudinal mode with a linewidth below 10 MHz and an optical output power of more than 300 mW at a wavelength of about 647 nm. The master‐oscillator is a distributed‐Bragg‐reflector ridge‐waveguide laser which provides the single mode with an output power of about 40 mW. The light is shaped by micro‐lenses and guided through a miniaturised CdMnTe‐Faraday isolator as well as a truncated tapered power amplifier; it is reshaped again afterwards to produce an almost circular, nearly diffraction‐limited beam. The total length of the MOPA is below 50 mm, which fitted into a sealed butterfly package with a footprint of about 20 cm2.
Promoting physical activity among children and youth in disadvantaged South Australian CALD communities through alternative community sport opportunities
Authors: Rosso Edoardo , McGrath Richard 
Published: February 2016

Issue addressedRecently arrived migrants and refugees from a culturally and linguistically diverse background (CALD) may be particularly vulnerable to social exclusion. Participation in sport is endorsed as a vehicle to ease the resettlement process; however, in Australia, this is often thought as a simple matter of integration into existing sport structures (e.g. clubs). This approach fails to place actual community needs at the centre of sport engagement efforts.MethodsA consultation framework was established with South Australian CALD community leaders and organisations to scope needs for community‐based alternatives to participation in traditional sport (e.g. clubs), co‐design a suitable community sport program and pilot it in five communities. Interviews and questionnaire surveys were conducted with participants, community representatives, stakeholders and volunteers.ResultsRegular, free soccer activities engaged 263 young people from a great variety of nationalities, including over 50% refugees, in secondary state school and community‐based sites.ConclusionAlternative community sport programs can provide a basic but valuable forum to promote physical activity and associated well being in CALD and refugee communities.So what?Alternative approaches can extend the health benefits of sport participation to disadvantaged children and youth who are excluded from traditional sport participation opportunities.
Hobbes on the function of evaluative speech
Authors: Holden Thomas 
Published: February 2016

AbstractHobbes’s interpreters have struggled to find a plausible semantics for evaluative language in his writings. I argue that this search is misguided. Hobbes offers neither an account of the reference of evaluative terms nor a theory of the truth-conditions for evaluative statements. Rather, he sees evaluative language simply as having the non-representational function of prescribing actions and practical attitudes, its superficially representational appearance notwithstanding. I marshal the evidence for this prescriptivist reading of Hobbes on evaluative language and show how it sidesteps various textual and philosophical problems that bedevil the traditional interpretations.
Coptis japonicaMakino extract suppresses angiogenesis through regulation of cell cycle-related proteins
Authors: Kim Seo Ho , Kim Eok-Cheon , Kim Wan-Joong , Lee Myung-Hun , Kim Sun-Young , Kim Tack-Joong 
Published: February 2016

AbstractAngiogenesis, neovascularization from pre-existing vessels, is a key step in tumor growth and metastasis, and anti-angiogenic agents that can interfere with these essential steps of cancer development are a promising strategy for human cancer treatment. In this study, we characterized the anti-angiogenic effects of Coptis japonica Makino extract (CJME) and its mechanism of action. CJME significantly inhibited the proliferation, migration, and invasion of vascular endothelial growth factor (VEGF)-stimulated HUVECs. Furthermore, CJME suppressed VEGF-induced tube formation in vitro and VEGF-induced microvessel sprouting ex vivo. According to our study, CJME blocked VEGF-induced cell cycle transition in G1. CJME decreased expression of cell cycle-regulated proteins, including Cyclin D, Cyclin E, Cdk2, and Cdk4 in response to VEGF. Taken together, the results of our study indicate that CJME suppresses VEGF-induced angiogenic events such as proliferation, migration, and tube formation via cell cycle arrest in G1.
A Wiskott–Aldrich syndrome protein is involved in endocytosis in Aspergillus nidulans
Authors: Hoshi Hiro-omi , Zheng Lu , Ohta Akinori , Horiuchi Hiroyuki 
Published: February 2016

Abstract
Endocytosis is vital for hyphal tip growth in filamentous fungi and is involved in the tip localization of various membrane proteins. To investigate the function of a Wiskott–Aldrich syndrome protein (WASP) in endocytosis of filamentous fungi, we identified a WASP ortholog-encoding gene, wspA, in Aspergillus nidulans and characterized it. The wspA product, WspA, localized to the tips of germ tubes during germination and actin rings in the subapical regions of mature hyphae. wspA is essential for the growth and functioned in the polarity establishment and maintenance during germination of conidia. We also investigated its function in endocytosis and revealed that endocytosis of SynA, a synaptobrevin ortholog that is known to be endocytosed at the subapical regions of hyphal tips in A. nidulans, did not occur when wspA expression was repressed. These results suggest that WspA plays roles in endocytosis at hyphal tips and polarity establishment during germination.
Macrophage migration inhibitory factor in the regulation of myoblast proliferation and differentiation
Authors: Wen Fengyun , Zheng Jin , Yu Jing , Gao Mingju , Gao Sumin , Zhou Yingying , Liu Jianyu , Yang Zaiqing 
Published: February 2016

Abstract
Obesity is documented to be a state of chronic mild inflammation associated with increased macrophage infiltration into adipose tissue and liver and skeletal muscle. As a pleiotropic inflammatory mediator, macrophage migration inhibitory factor (MIF) is associated with metabolic disease, so MIF may signal molecular links between adipocytes and myocytes. MIF expression was modified during myoblast differentiation, but the role of MIF during this process is unclear. C2C12 cells were transfected with MIF to investigate their role during differentiation. MIF expression attenuated C2C12 differentiation. It did not change proliferation, but downregulated cyclin D1 and CDK4, causing cell accumulation in the G1 phase. p21 protein was increased significantly and MyoD, MyoG, and p21 mRNA also increased significantly in the C2C12 cells treated with ISO-1, suggesting that inhibition of MIF promotes differentiation. MIF inhibits the myoblast differentiation by affecting the cell cycle progression, but does not affect proliferation.
An alternative class of targets for microRNAs containing CG dinucleotide
Authors: Yan Zheng , Zhang Bin , Hu Haiyang , Xie Gangcai , Guo Song , Khaitovich Philipp , Chen Yi-Ping Phoebe 
Published: February 2016

AbstractBackgroundMicroRNAs are endogenous ∼23nt RNAs which regulate mRNA targets mainly through perfect pairing with their seed region (positions 2-7). Several instances of bulge UTR sequence can also be recognized by miRNA as their target. But such non-Watson-Crick base pairings are incompletely understood.ResultsWe found a group of miRNAs which had very few conservative targets while potentially having a subclass of bulge message RNA targets. Compared with the canonical target, these bulge targets had a lower negative correlation with the miRNA expression, and either were downregulated in the miRNA overexpression experiment or upregulated in the miRNA knock-down experiment.ConclusionsWe proved that the bulge target exists widely in certain groups of miRNAs and such non-canonical targets can be recoginized by miRNA. Incorporating these bulge targets, combined with evolutionary conservation, will reduce the false-positive rate of microRNA computational target prediction.
A Dendritic Disinhibitory Circuit Mechanism for Pathway-Specific Gating
Authors: Yang Guangyu Robert , Murray John D. , Wang Xiao-Jing 
Published: February 2016

SummaryIn this work we propose that a disinhibitory circuit motif, which recently gained experimental support, can instantiate flexible routing of information flow along selective pathways in a complex system of cortical areas according to behavioral demands (pathway-specific gating). We developed a network model of pyramidal neurons and three classes of interneurons, with connection probabilities constrained by data. If distinct input pathways cluster on separate dendritic branches of pyramidal neurons, then a pathway can be gated-on by disinhibiting targeted dendrites. We show that this branch-specific disinhibition can be achieved despite dense in-terneuronal connectivity, even under the assumption of random connections. We found clustering of input pathways on dendrites can emerge through synaptic plasticity regulated by disinhibition. This gating mechanism in a neural circuit is further demonstrated by performing a context-dependent decision-making task. Our findings suggest a microcircuit architecture that harnesses dendritic computation and diverse inhibitory neuron types to subserve cognitive flexibility.
Factors related to supply chain network members in SMEs
Authors: Awheda Abdulghadar , Ab Rahman Mohd Nizam , Ramli Rizauddin , Arshad Haslina 
Published: February 2016

Purpose
– The purpose of this paper is to give a clear picture of the evidence for small and medium enterprises of what the key factors are affecting the performance of supply chain members (SCMs) and their impact.

Design/methodology/approach
– The authors conducted a survey of small and medium automotive enterprises. After a comprehensive search of the literature on effective factors requirements of supply chain actors (supplier, manufacturer, inventory, distribution, and information flow) 23 factors were identified, and collected data were analysed using SPSS software.

Findings
– The results show that among the 23 factors, the inventory level is the most significant factor for inventory with the highest percentage (91 per cent). This is followed by productivity to fill the order (88 per cent) and lead time of manufacturing (86 per cent) for manufacturer, and transportation flexibility (84 per cent) for distribution section.

Research limitations/implications
– Study limitation was that only the companies related to automotive sector were selected, and the number of usable questionnaires was fairly small.

Practical implications
– The results can help further by introducing a much better perception of effective factors that result in optimizing the practices of supply chain management techniques in order to enhance business performance. The findings also help build a new initiative to be performed by top management when attempting to strengthen partnership and integration among members.

Originality/value
– This research draws from institutional theory to contribute to the growing body of practical field and conceptual literature on supply chain integration and provides knowledge in assisting small- and medium-sized enterprises to clarify factors that affect SCMs in order to enhance and manage their operational processes.