bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poisson Mesh Reconstruction for Accurate Object Tracking With Low-Fidelity Point Clouds
Authors: Garrett Timothy , Debernardis Saverio , Oliver James , Radkowski Rafael 
Published: July 2016

Abstract
Tracking refers to a set of techniques that allows one to calculate the position and orientation of an object with respect to a global reference coordinate system in real time. A common method for tracking with point clouds is the iterative closest point (ICP) algorithm, which relies on the continuous matching of sequential sampled point clouds with a reference point cloud. Modern commodity range cameras provide point cloud data that can be used for that purpose. However, this point cloud data is generally considered as low-fidelity and insufficient for accurate object tracking. Mesh reconstruction algorithms can improve the fidelity of the point cloud by reconstructing the overall shape of the object. This paper explores the potential for point cloud fidelity improvement via the Poisson mesh reconstruction (PMR) algorithm and compares the accuracy with a common ICP-based tracking technique and a local mesh reconstruction operator. The results of an offline simulation are promising.
Employing Adaptive Mesh Refinement for Simulating the Exhaust Gas Recirculation Mixing Process
Authors: Bramlette Richard , Langness Chenaniah , Mangus Michael , Depcik Christopher 
Published: July 2016

One significant emissions issue of compression ignition engines that directly influences human health is the production of nitrogen oxides (NOx). Once produced, these species are difficult to convert catalytically in the exhaust and often require a complex aftertreatment system to mitigate their release into the environment. The common methodology by the internal combustion engine community to reduce the amount of NOx is to employ Exhaust Gas Recirculation (EGR) in order to dilute the intake mixture with inert species (e.g., water). This lowers the combustion temperature lessening the thermal NO production mechanism. Improper mixing of EGR with the intake (species in-homogeneity, low levels of mixing turbulence, etc.) can lead to significant cylinder-to-cylinder variation in combustion temperatures and NOx emissions, making it more difficult to achieve regulatory standards.
In this effort, a three-dimensional (3-D), transient, computational fluid dynamics (CFD) analysis was performed in order to more accurately model the mixing of EGR and intake for a single-cylinder test engine. Mixing is achieved for this engine by using a small rectangular box in which clean air and engine exhaust for controlled recirculation are mixed prior to engine intake. A matrix of computational analyses at different engine loads, and simulation types (large eddy and Reynolds-averaged Navier-Stokes) at 25% EGR were performed to check computational time and agreement with experimental measurements. Moreover, this effort employs the use of adaptive mesh techniques in order to understand their usage and validate correct implementation for later endeavors including more complex geometries, such as the manifold of a multi-cylinder engine. The simulation results indicate that mass flow rate and temperature of the mixture as it leaves the mixing box agree to within 3% of experimental values. Furthermore, pressures at the air and EGR inlet boundaries showed agreement to around 1% and 12%, respectively, with the experimental measuring points indicated as the reason for the difference. In addition, species mixing of carbon monoxide was uniform to within 440 ppm. Finally, the use of the models may also account for a prior discrepancy in the output power of the single-cylinder engine test stand.
Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of Polyreactivity
Authors: Donius Luke R. , Cheng Yuxing , Choi Jaewon , Sun Zhen-Yu J. , Hanson Melissa , Zhang Michael , Gierahn Todd M. , Marquez Susanna , Uduman Mohammed , Kleinstein Steven H. , Irvine Darrell , Love J. Christopher , Reinherz Ellis L. , Kim Mikyung 
Published: July 2016

ABSTRACT
An effective preventive vaccine is highly sought after in order to stem the current HIV-1 pandemic. Both conservation of contiguous gp41 membrane-proximal external region (MPER) amino acid sequences across HIV-1 clades and the ability of anti-MPER broadly neutralizing antibodies (BNAbs) to block viral hemifusion/fusion establish the MPER as a prime vaccination target. In earlier studies, we described the development of an MPER vaccine formulation that takes advantage of liposomes to array the MPER on a lipid bilayer surface, paralleling its native configuration on the virus membrane while also incorporating molecular adjuvant and CD4 T cell epitope cargo. Here we demonstrate that several immunizations with MPER/liposomes induce high levels of bone marrow long-lived plasma cell (LLPC) antibody production. Single-cell immunoglobulin gene retrieval analysis shows that these plasma cells are derived from a germ line repertoire of B cells with a diverse representation of immunoglobulin genes, exhibiting antigen-driven positive selection. Characterization of LLPC recombinant monoclonal antibodies (rMAbs) indicates that antigen recognition is achieved through convergence on a common epitopic focus by utilizing various complementarity-determining region H3 (CDRH3) lengths. Importantly, the vast majority of rMAbs produced from these cells lack polyreactivity yet manifest antigen specificity in the context of lipids, shaping MPER-specific paratopes through selective pressure. Taken together, these findings demonstrate that the MPER is a vaccine target with minimal risk of generating off-target autoimmunity.

IMPORTANCE
A useful vaccine must generate desired long-term, antigen-specific antibody responses devoid of polyreactivity or autoreactivity. The common polyreactive features of some HIV-1 BNAbs have raised concern about elicitation of anti-MPER antibodies. Utilizing single-LLPC repertoire analysis and biophysical characterization of anti-MPER rMAbs, we show that their fine specificities require a structural fitness of the antibody combining site involving heavy and light chain variable domains shaped by somatic hypermutation and affinity maturation of B cells in the germinal center. Perhaps more importantly, our results demonstrate that the majority of MPER-specific antibodies are not inherently polyspecific and/or autoreactive, suggesting that polyreactivity of MPER-specific antibodies is separable from their antigen specificity.
Protocol for study of financial incentives for smoking cessation in pregnancy (FISCP): randomised, multicentre study
Authors: Berlin Noémi , Goldzahl Léontine , Jusot Florence , Berlin Ivan 
Published: July 2016

IntroductionMaternal smoking during pregnancy is associated with adverse perinatal and postnatal health outcomes. The efficacy of nicotine replacement therapies in helping pregnant smokers to quit is not clearly demonstrated; therefore new interventions should be proposed and assessed. Financial incentives rewarding abstinence from tobacco smoking is one of the promising options.ObjectiveTo assess the efficacy of financial incentives on smoking abstinence among French pregnant smokers.Methods and analysisParticipants:pregnant smokers aged ≥18 years, smoking at least five manufactured or three roll-your-own cigarettes per day, and pregnant for <18 weeks of amenorrhoea (WA).Setting:participants will be recruited, included and followed-up at monthly face-to-face visits in 16 maternity wards in France.Interventions:participants will be randomised to a control or an intervention group. After a predefined quit date, participants in the control group will receive €20 vouchers at the completion of each visit but no financial incentive for smoking abstinence. Participants in the intervention group will be rewarded for their abstinence by vouchers on top of the €20 show-up fee. The amount of reward for abstinence will increase as a function of duration of abstinence to stimulate longer periods of abstinence.Main outcome measure: complete abstinence from quit date to the last predelivery visit.Secondary outcome measures:point prevalence abstinence, time to relapse to smoking, birth weight, fetal growth restriction, preterm birth.Main data analysis:outcomes will be analysed on an intention-to-treat (ITT) basis. The ITT population is defined as all randomised smoking pregnant women.Ethics and disseminationThe research protocol was approved by the ethics committee (Comité de Protection des Personnes, CPP) of the Pitié-Salpêtrière Hospital on 15 May 2015, and Amendment No 1 was approved on 13 July 2015. Results will be presented at scientific meetings and published.Trial registration numberNCT02606227; Pre-results.
Genomic ancestry as a predictor of haemodynamic profile in heart failure
Authors: Bernardez-Pereira Sabrina , Gioli-Pereira Luciana , Marcondes-Braga Fabiana G , Santos Paulo Caleb Junior Lima , Spina Joceli Mabel Rocha , Horimoto Andréa Roseli Vançan Russo , Santos Hadassa Campos , Bacal Fernando , Fernandes Fábio , Mansur Alfredo Jose , Pietrobon Ricardo , Krieger José Eduardo , Mesquita Evandro Tinoco , Pereira Alexandre Costa 
Published: July 2016

ObjectiveThe aim of this study is to assess the association between genetic ancestry, self-declared race and haemodynamic parameters in patients with chronic heart failure (HF).MethodsObservational, cross-sectional study. Eligible participants were aged between 18 and 80 years; ejection fraction was ≤50%. Patients underwent genetic analysis of ancestry informative markers, echocardiography and impedance cardiography (ICG). Race was determined by self-classification into two groups: white and non-white. Genomic ancestry was estimated using a panel of 101 348 polymorphic markers and three continental reference populations (European, African and Native American).ResultsOur study included 362 patients with HF between August 2012 and August 2014. 123 patients with HF declared themselves as white and 234 patients declared themselves as non-white. No statistically significant differences were found regarding the ICG parameters according to self-declared race. The Amerindian ancestry was positively correlated with systolic time ratio (r=0.109, p<0.05). The thoracic fluid content index (r=0.124. p<0.05), E wave peak (r=0.127. p<0.05) and E/e′ ratio (r=0.197. p<0.01) were correlated positively with African ancestry. In multiple linear regression, African ancestry remained associated with the E/e′ ratio, even after adjustment to risk factors.ConclusionsThe African genetic ancestry was associated with worse parameters of diastolic function; the Amerindian ancestry correlated with a worse pattern of ventricular contractility, while self-declared colour was not helpful to infer haemodynamic profiles in HF.Trials registration numberNTC02043431.
Educational needs and preferences of young European clinicians and physician researchers working in the field of rheumatology
Authors: Beyer Christian , Ramiro Sofia , Sivera Francisca , Mandl Peter , Machado Pedro M , Ospelt Caroline , Moltó Anna , Radner Helga , Iagnocco Annamaria , Bijlsma Johannes W , Lundberg Ingrid E 
Published: July 2016

ObjectivesTo understand the educational needs and preferences of young clinicians and physician researchers in the field of rheumatology in Europe.MethodsAn international online survey was performed as a joint venture of ESCET and EMEUNET. The survey assessed the acceptance of and the access to the current European League Against Rheumatism (EULAR) educational portfolio, as well as the unmet educational needs and learning preferences among individuals below the age of 40 years working in rheumatology in Europe.ResultsAmong 568 European clinicians and physician researchers, 65% indicated that the existing EULAR educational portfolio adequately covers their educational needs. Within the EULAR portfolio, the online course on rheumatic diseases and the postgraduate course were the most appreciated. Participants were very much in favour of new educational courses on imaging techniques, and 63% of participants indicated a particular interest in musculoskeletal ultrasound. A strong interest in refresher (60%) and general review (55%) courses was observed. Lack of funding was considered the major obstacle to participating in existing EULAR programmes. Finally, participants showed diverse preferences regarding learning modalities with common interests in live courses and conferences.ConclusionsEULAR's training opportunities are well appreciated among young clinicians and physician researchers in rheumatology. The results from this survey will help to develop EULAR's future educational portfolio.
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells
Authors: Moraes Daniela A. , Sibov Tatiana T. , Pavon Lorena F. , Alvim Paula Q. , Bonadio Raphael S. , Da Silva Jaqueline R. , Pic-Taylor Aline , Toledo Orlando A. , Marti Luciana C. , Azevedo Ricardo B. , Oliveira Daniela M. 
Published: July 2016

Abstract
Background
Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs.

Methods
We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors.

Results
The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression.

Conclusion
Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro.
Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters
Authors: Stanly Tess A. , Fritzsche Marco , Banerji Suneale , García Esther , Bernardino de la Serna Jorge , Jackson David G. , Eggeling Christian 
Published: July 2016

ABSTRACTReceptor clustering is known to trigger signalling events that contribute to critical changes in cellular functions. Faithful imaging of such clusters by means of fluorescence microscopy relies on the application of adequate cell fixation methods prior to immunolabelling in order to avoid artefactual redistribution by the antibodies themselves. Previous work has highlighted the inadequacy of fixation with paraformaldehyde (PFA) alone for efficient immobilisation of membrane-associated molecules, and the advantages of fixation with PFA in combination with glutaraldehyde (GA). Using fluorescence microscopy, we here highlight how inadequate fixation can lead to the formation of artefactual clustering of receptors in lymphatic endothelial cells, focussing on the transmembrane hyaluronan receptors LYVE-1 and CD44, and the homotypic adhesion molecule CD31, each of which displays their native diffuse surface distribution pattern only when visualised with the right fixation techniques, i.e. PFA/GA in combination. Fluorescence recovery after photobleaching (FRAP) confirms that the artefactual receptor clusters are indeed introduced by residual mobility. In contrast, we observed full immobilisation of membrane proteins in cells that were fixed and then subsequently permeabilised, irrespective of whether the fixative was PFA or PFA/GA in combination. Our study underlines the importance of choosing appropriate sample preparation protocols for preserving authentic receptor organisation in advanced fluorescence microscopy.
A role for the yeast CLIP170 ortholog, the plus-end-tracking protein Bik1, and the Rho1 GTPase in Snc1 trafficking
Authors: Boscheron Cécile , Caudron Fabrice , Loeillet Sophie , Peloso Charlotte , Mugnier Marine , Kurzawa Laetitia , Nicolas Alain , Denarier Eric , Aubry Laurence , Andrieux Annie 
Published: July 2016

ABSTRACT
The diversity of microtubule functions is dependent on the status of tubulin C-termini. To address the physiological role of the C-terminal aromatic residue of α-tubulin, a tub1-Glu yeast strain expressing an α-tubulin devoid of its C-terminal amino acid was used to perform a genome-wide-lethality screen. The identified synthetic lethal genes suggested links with endocytosis and related processes. In the tub1-Glu strain, the routing of the v-SNARE Snc1 was strongly impaired, with a loss of its polarized distribution in the bud, and Abp1, an actin patch or endocytic marker, developed comet-tail structures. Snc1 trafficking required dynamic microtubules but not dynein and kinesin motors. Interestingly, deletion of the microtubule plus-end-tracking protein Bik1 (a CLIP170 ortholog), which is preferentially recruited to the C-terminal residue of α-tubulin, similarly resulted in Snc1 trafficking defects. Finally, constitutively active Rho1 rescued both Bik1 localization at the microtubule plus-ends in tub1-Glu strain and a correct Snc1 trafficking in a Bik1-dependent manner. Our results provide the first evidence for a role of microtubule plus-ends in membrane cargo trafficking in yeast, through Rho1- and Bik1-dependent mechanisms, and highlight the importance of the C-terminal α-tubulin amino acid in this process.
Integration of leadership competencies in a community health simulation
Authors: R Martin Dana , B Furr Susan , Hayes Lane Susan , Bramlett Martha 
Published: July 2016

Aim: The purpose of this article is to address ways in which a nursing faculty can build nursing leadership competencies into a nursing programme. This article describes the use of simulation as a compelling learning strategy. Background: Nurses need to be prepared to meet the demands of mass casualty events. With the growing need for major incident training, leadership competency development is a critical gap in the literature. Evaluation: A collaborative simulation was used to help students apply classroom knowledge. In this simulation, students participated in major incident triage and the application of leadership competencies to appropriately manage victims' care. Conclusion: Collaboration among faculty members when designing simulation scenarios is a powerful approach to nursing education. Students were able to engage in necessary skills for their future nursing practice.
The effect of fiber-rich milk and equi-carbohydrate snack on glycemic and insulin response and satiety feeling
Authors: Chandra Dian N. , Bardosono Saptawati 
Published: July 2016

Background: Additional dietary fibers which can decrease the glycemic response by slowing down digestion whilst maintaining the available carbohydrate content is one approach of healthy diet. This study aimed to compare post-prandial glycemic and insulin response, hunger and satiety feeling after consuming fiber-rich milk compare with equi-carbohydrate food as morning snack in healthy adults.Methods: Cross-over study was conducted on 12 healthy subjects who fulfilled the criteria. Each test food was given after consuming standard breakfast. Venous blood samples for insulin and glucose level were taken before consuming test food, at 30, 60, 120, and 180 minutes after, and plotted against time to generate a curve. Hunger and satiety assessments were taken by visual analog scale (VAS) after each blood sampling.Results: In average, age was 30.8+4.3 years old, body mass index was 20.6±1.6 kg/m2. Seven of twelve subjects were females. There were significantly differences in postprandial glycemic response (p<0.001), insulin response (p=0.045) and hunger feeling (p=0.021) between the two foods. However, postprandial satiety feelings were not different significantly (p=0.357). The glycemic response area under the curve of fiber-rich milk was significantly lower than the equi-carbohydrate snack (p=0.010). Conclusion: Differences in glycemic and insulin response, and hunger feeling between two test foods, suggesting that fiber-rich milk can be used as an alternative snack for healthy adults. Further study is needed for the use of fiber-rich milk as an alternative snack for pre-diabetic patients.
Synthesis and Characterization of a New Coordinative Compound of Cu(II) with 1-(3 Bromo, 2 Hydroxy, 4 Methylphenyl)- 2-(4 Bromo-Phenyl-Sulphanyl)- Etanone
Authors: Antighin Simona , Chirilă Laura 
Published: July 2016

Abstract
Continuing the research in the field of complex compounds, the authors present in this paper the synthesis and characterization of a new compound of Cu(II) with the ligand 1-(3 bromo, 2 hydroxy, 4 methyl-phenyl)-2-(4 bromophenyl- sulphanyl)-etanone. Different methods were used, such as chemical elemental analysis, thermal-gravimetry, infrared absorption spectroscopy and electronic spin resonance (ESR). From chemical analysis resulted that the combination ratio ligand-central atom is 2:1. The new compound can also be used for gravimetric determination of Cu(II).
Differential Pulse Anodic Stripping Voltammetry for Mercury Determination
Authors: Vereștiuc Paul C. , Tucaliuc Oana-Maria , Breabăn Iuliana G. , Crețescu Igor , Nemțoi Gheorghe 
Published: July 2016

Abstact
In the present work voltammetric investigations have been performed on HgCl2 aqueous solutions prepared from a Cz 9024 reagent. Carbon paste electrode (CPE), eriochrome black T modified carbon paste electrode (MCPE/EBT) and KCl 1M as background electrolyte, were involved within the experimental procedures. Cyclic voltammetry (CV) has been performed in order to compare the behaviour of the two electrodes in both K3[Fe(CN)6] and mercury calibration aqueous solution. Differential pulse anodic stripping voltammetry (DPASV) was used to determine the most suitable parameters for mercury determination. All experiments were performed at 25 ± 1 ℃, using an electrochemical cell with three-electrodes connected to an Autolab PG STAT 302N (Metrohm-Autolab) potentiostat that is equipped with Nova 1.11 software. The measured potential values were generated by using the silver chloride electrode (AgClE) as reference and a platinum wire electrode as auxiliary. A series of time depending equations for the pre-concentration and concentration steps were established, with the observation that a higher sensitivity can be obtained while increasing the pre-concentration time. DPASV were drawn using the CPE in 11.16 % coriander, as mercury complex, the voltamograms signals indicating mercury oxidation, with signal intensity increasing in time.
Comparative Study of Creatinine and Urea in the Case of Well and Poorly Controlled Diabetes
Authors: Mihaela Gonțea , Elena Olteanu Grigoruț , Dănuț Cozma G. 
Published: July 2016

Abstract
The glycosylated hemoglobin is useful in monitoring diabetes regardless of its type allowing a retroactive evaluation over a period of 45 days according to the last studies, being a suggestive mirror for the glycemic status. The protein metabolism is evaluated through biochemical determinations of creatinine and urea. The medical practice is interested in how ensuring a good control or a less good control, of diabetes, will generate changes in the case of these biochemical parameters.