bluuebunny/crossref_metadata_2025_split huggingface dataset:
XBP1 splicing triggers miR-150 transfer from smooth muscle cells to endothelial cells via extracellular vesicles
Authors: Zhao Yue , Li Yi , Luo Peiyi , Gao Yingtang , Yang Junyao , Lao Ka-Hou , Wang Gang , Cockerill Gillian , Hu Yanhua , Xu Qingbo , Li Tong , Zeng Lingfang 
Published: June 2016

AbstractThe interaction between endothelial cells (ECs) and smooth muscle cells (SMCs) plays a critical role in the maintenance of vessel wall homeostasis. The X-box binding protein 1 (XBP1) plays an important role in EC and SMC cellular functions. However, whether XBP1 is involved in EC-SMC interaction remains unclear. In this study, In vivo experiments with hindlimb ischemia models revealed that XBP1 deficiency in SMCs significantly attenuated angiogenesis in ischemic tissues, therefore retarded the foot blood perfusion recovery. In vitro studies indicated that either overexpression of the spliced XBP1 or treatment with platelet derived growth factor-BB up-regulated miR-150 expression and secretion via extracellular vesicles (EVs). The XBP1 splicing-mediated up-regulation of miR-150 might be due to increased stability. The SMC-derived EVs could trigger EC migration, which was abolished by miR-150 knockdown in SMCs, suggesting miR-150 is responsible for SMC-stimulated EC migration. The SMC-derived miR-150-containing EVs or premiR-150 transfection increased vascular endothelial growth factor (VEGF)-A mRNA and secretion in ECs. Both inhibitors SU5416 and LY294002 attenuated EVs-induced EC migration. This study demonstrates that XBP1 splicing in SMCs can control EC migration via SMC derived EVs-mediated miR-150 transfer and miR-150-driven VEGF-A/VEGFR/PI3K/Akt pathway activation, thereby modulating the maintenance of vessel wall homeostasis.
Increased CXCR3 Expression of Infiltrating Plasma Cells in Hunner Type Interstitial Cystitis
Authors: Akiyama Yoshiyuki , Morikawa Teppei , Maeda Daichi , Shintani Yukako , Niimi Aya , Nomiya Akira , Nakayama Atsuhito , Igawa Yasuhiko , Fukayama Masashi , Homma Yukio 
Published: June 2016

AbstractAn up-regulated CXCR3 pathway and affluent plasma cell infiltration are characteristic features of Hunner type interstitial cystitis (HIC). We further examined these two features using bladder biopsy samples taken from 27 patients with HIC and 15 patients with non-IC cystitis as a control. The number of CD3-positive T lymphocytes, CD20-positive B lymphocytes, CD138-positive plasma cells, and CXCR3-positive cells was quantified by digital image analysis. Double-immunofluorescence for CXCR3 and CD138 was used to detect CXCR3 expression in plasma cells. Correlations between CXCR3 positivity and lymphocytic and plasma cell numbers and clinical parameters were explored. The density of CXCR3-positive cells showed no significant differences between HIC and non-IC cystitis specimens. However, distribution of CXCR3-positivity in plasma cells indicated co-localization of CXCR3 with CD138 in HIC specimens, but not in non-IC cystitis specimens. The number of CXCR3-positive cells correlated with plasma cells in HIC specimens alone. Infiltration of CXCR3-positive cells was unrelated to clinical parameters of patients with HIC. These results suggest that infiltration of CXCR3-positive plasma cells is a characteristic feature of HIC. The CXCR3 pathway and specific immune responses may be involved in accumulation/retention of plasma cells and pathophysiology of the HIC bladder.
Efficiency Enhancement of InGaN-Based Solar Cells via Stacking Layers of Light-Harvesting Nanospheres
Authors: Al-Amri Amal M. , Fu Po-Han , Lai Kun-Yu , Wang Hsin-Ping , Li Lain-Jong , He Jr-Hau 
Published: June 2016

AbstractAn effective light-harvesting scheme for InGaN-based multiple quantum well solar cells is demonstrated using stacking layers of polystyrene nanospheres. Light-harvesting efficiencies on the solar cells covered with varied stacks of nanospheres are evaluated through numerical and experimental methods. The numerical simulation reveals that nanospheres with 3 stacking layers exhibit the most improved optical absorption and haze ratio as compared to those obtained by monolayer nanospheres. The experimental demonstration, agreeing with the theoretical analyses, shows that the application of 3-layer nanospheres improves the conversion efficiency of the solar cell by ~31%.
Intestinally-targeted TGR5 agonists equipped with quaternary ammonium have an improved hypoglycemic effect and reduced gallbladder filling effect
Authors: Cao Hua , Chen Zhi-Xiang , Wang Kai , Ning Meng-Meng , Zou Qing-An , Feng Ying , Ye Yang-Liang , Leng Ying , Shen Jian-Hua 
Published: June 2016

AbstractTGR5 activation of enteroendocrine cells increases glucagon-like peptide 1 (GLP-1) release, which maintains glycemic homeostasis. However, TGR5 activation in the gallbladder and heart is associated with severe side effects. Therefore, intestinally-targeted TGR5 agonists were suggested as potential hypoglycemic agents with minimal side effects. However, until now no such compounds with robust glucose-lowering effects were reported, especially in diabetic animal models. Herein, we identify a TGR5 agonist, 26a, which was proven to be intestinally-targeted through pharmacokinetic studies. 26a was used as a tool drug to verify the intestinally-targeted strategy. 26a displayed a robust and long-lasting hypoglycemic effect in ob/ob mice (once a day dosing (QD) and 18-day treatment) owing to sustained stimulation of GLP-1 secretion, which suggested that robust hypoglycemic effect could be achieved with activation of TGR5 in intestine alone. However, the gallbladder filling effect of 26a was rather complicated. Although the gallbladder filling effect of 26a was decreased in mice after once a day dosing, this side effect was still not eliminated. To solve the problem above, several research strategies were raised for further optimization.
Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration
Authors: Naranda Jakob , Sušec Maja , Maver Uroš , Gradišnik Lidija , Gorenjak Mario , Vukasović Andreja , Ivković Alan , Rupnik Marjan Slak , Vogrin Matjaž , Krajnc Peter 
Published: June 2016

AbstractDevelopment of artificial materials for the facilitation of cartilage regeneration remains an important challenge in orthopedic practice. Our study investigates the potential for neocartilage formation within a synthetic polyester scaffold based on the polymerization of high internal phase emulsions. The fabrication of polyHIPE polymer (PHP) was specifically tailored to produce a highly porous (85%) structure with the primary pore size in the range of 50–170 μm for cartilage tissue engineering. The resulting PHP scaffold was proven biocompatible with human articular chondrocytes and viable cells were observed within the materials as evaluated using the Live/Dead assay and histological analysis. Chondrocytes with round nuclei were organized into multicellular layers on the PHP surface and were observed to grow approximately 300 μm into the scaffold interior. The accumulation of collagen type 2 was detected using immunohistochemistry and chondrogenic specific genes were expressed with favorable collagen type 2 to 1 ratio. In addition, PHP samples are biodegradable and their baseline mechanical properties are similar to those of native cartilage, which enhance chondrocyte cell growth and proliferation.
A practical online tool to estimate antiretroviral coverage for HIV infected and susceptible populations needed to reduce local HIV epidemics
Authors: Chaillon Antoine , Hoenigl Martin , Mehta Sanjay R. , Weibel Nadir , Little Susan J. , Smith Davey M. 
Published: June 2016

AbstractIt remains unclear what proportions of HIV-infected and uninfected people should receive effective antiretroviral therapy (ART) to control local HIV epidemics. We developed a flexible model to evaluate the impact of treatment as prevention (TasP) and pre-exposure prophylaxis (PrEP) on HIV incidence in local communities. We evaluated this tool for determining what TasP and PrEP targets are needed to substantially reduce the HIV epidemic in San Diego, which is predominately comprised of men who have sex with men. By increasing the proportion of HIV-infected individuals on ART from 30% to 50%, 686 new infections would be prevented over five years in San Diego. By providing PrEP to 30% of MSM to the age group that account for 90% of local HIV incident cases (21–52 years), we could prevent 433 infections over five years. When combining these initiatives, a PrEP coverage rate of 40% and TasP coverage rate of 34% would be expected to decrease the number of new infections by over half in one year. This online tool is designed to help local public health planners and policy makers to estimate program outcomes and costs that may lead to better control of their local HIV epidemics.
Proteasome regulates turnover of toxic human amylin in pancreatic cells
Authors: Singh Sanghamitra , Trikha Saurabh , Sarkar Anjali , Jeremic Aleksandar M. 
Published: June 2016

Toxic human amylin (hA) oligomers and aggregates are implicated in the pathogenesis of type 2 diabetes mellitus (T2DM). Although recent studies demonstrated a causal connection between hA uptake and toxicity in pancreatic cells, the mechanism of amylin's clearance following its internalization and its relationship to toxicity is yet to be determined, and hence was investigated here. Using pancreatic rat insulinoma β-cells and human islets as model systems, we show that hA, following its internalization, first accumulates in the cytosol followed by its translocation into nucleus, and to a lesser extent lysosomes, keeping the net cytosolic amylin content low. An increase in hA accumulation in the nucleus of pancreatic cells correlated with its cytotoxicity, suggesting that its excessive accumulation in the nucleus is detrimental. hA interacted with 20S core and 19S lid subunits of the β-cell proteasomal complex, as suggested by immunoprecipitation and confocal microscopy studies, which subsequently resulted in a decrease in the proteasome's proteolytic activity in these cells. In vitro binding and activity assays confirmed an intrinsic and potent ability of amylin to interact with the 20S core complex thereby modulating its proteolytic activity. Interestingly, less toxic and aggregation incapable rat amylin (rA) showed a comparable inhibitory effect on proteasome activity and protein ubiquitination, decoupling amylin aggregation/ toxicity and amylin-induced protein stress. In agreement with these studies, inhibition of proteasomal proteolytic activity significantly increased intracellular amylin content and toxicity. Taken together, our results suggest a pivotal role of proteasomes in amylin's turnover and detoxification in pancreatic cells.
Modelling of the modified‐LLCL‐filter‐based single‐phase grid‐tied Aalborg inverter
Authors: Liu Zifa , Wu Huiyun , Liu Yuan , Ji Junhao , Wu Weimin , Blaabjerg Frede 
Published: June 2016

Owing to less conduction and switching power losses, the recently proposed Aalborg inverter has high efficiency within a wide range of input DC voltage for single‐phase DC/AC power conversion. In theory, the conduction power losses can be further decreased, if an LLCL‐filter is adopted instead of an LCL‐filter for a voltage source inverter, mainly due to the reduced inductance. The Aalborg inverter shows the characteristic of a current source inverter, when working in the ‘boost’ state. Whether the LLCL‐filter can meet the control requirement of this type inverter needs to be further explored. In this study, the small signal analysis for the modified‐LLCL‐filter‐based Aalborg inverter is addressed. Through the modelling, it can be proven that compared with the LCL‐filter, the modified‐LLCL‐filter causes no extra control challenge for the Aalborg inverter, and therefore more inductance in the power loop can be safely saved as well as a complete smaller filter size. A 220 V/50 Hz, 2000 W prototype is used to demonstrate the theoretical analysis.
Effects of dense code-switching on executive control
Authors: Hofweber Julia , Marinis Theodoros , Treffers-Daller Jeanine 
Published: June 2016

Abstract
Bilingualism is reported to re-structure executive control networks, but it remains unknown which aspects of the bilingual experience cause this modulation. This study explores the impact of three code-switching types on executive functions: (1) alternation, (2) insertion, and (3) dense code-switching or congruent lexicalisation. Current models hypothesise that different code-switching types challenge different aspects of the executive system because they vary in the extent and scope of language separation. Two groups of German-English bilinguals differing in dense code-switching frequency participated in a flanker task under conditions varying in degree of trial-mixing and resulting demands to conflict-monitoring. Bilinguals engaging in more dense code-switching showed inhibitory advantages in the condition requiring most conflict-monitoring. Moreover, dense code-switching frequency correlated positively with monitoring skills. This suggests that dense code-switching is a key experience shaping bilinguals’ executive functioning and highlights the importance of controlling for participants’ code-switching habits in bilingualism research.
Effects of various concentrations of carbohydrate mouth rinse on cycling performance in a fed state
Authors: Devenney Simon , Collins Kieran , Shortall Marcus 
Published: June 2016

AbstractThe objective of this study was to identify the effects of mouth rinsing with a 6% and 16% carbohydrate solution (CHO) on time trial performance when compared to a 0% control (PLA) when in a fed state. Twelve recreationally active males underwent three trials by which they had to complete a set workload (600 ± 65 W) in a fed state. Throughout each trial, participants rinsed their mouths with a 25 ml bolus of a 0% PLA, 6% or 16% CHO (maltodextrin) for every 12.5% of work completed. Rating of perceived exertion (RPE) and heart rate were recorded every 12.5% of total work. Performance times and power output improved significantly when using the 6% and 16% CHO versus the PLA trial (6% versus PLA, p = .002 and 16% versus PLA, p = .001). When comparing the performance times of the 6% to 16% CHO, no significance was observed (p = .244). There was no significant difference between heart rate levels or RPE values across the three trials. In conclusion, mouth rinsing with a 6% or 16% CHO solution has a positive effect on a cycling time trial performance undertaken in a fed state.
Biogeography and evolutionary diversification in one of the most widely distributed and species rich genera of the Pacific
Authors: Cantley Jason T. , Markey Adrienne S. , Swenson Nathan G. , Keeley Sterling C. 
Published: June 2016

AbstractThe historical biogeography of many lineages—of both terrestrial and marine ocean habitats—remains poorly investigated even though remote ocean habitat covers approximately 66% of the Earth’s surface. One such lineage with poorly understood biogeographic affinities across vast ocean habitat is the genus Coprosma (Rubiaceae) with numerous species, and a widespread and disjunct distribution among the far-flung insular localities of multiple Pacific Islands. Here, the first taxonomically robust phylogeny for Coprosma s.s. was dated using molecular clock techniques and indicated Coprosma s.s. diverged from its sister genus Nertera likely during or shortly after the Oligocene Marine Transgression of New Zealand. Diversification of the five major clades identified occurred in New Zealand during the Miocene, which was then followed by multiple independent dispersals from New Zealand to various localities in many directions. The pattern of Coprosma ’s distribution in the Pacific appears stochastic both temporally and spatially, but evolution of an orange to red fruit colour prior to nearly all inferred dispersals hints at endozoochory by birds. The number of inferred long-distance dispersals of Coprosma s.s. (&gt;30), and number of repeated dispersals to the same insular locality from unrelated Coprosma s.s. sublineages (&gt;8) is perhaps the most currently known for a remote Pacific-centred genus investigated to date. A New Zealand origin for a Pacific-wide dispersal of taxa is not novel, but the manner in which the temporal and spatial distribution for Coprosma s.s. was achieved contributes to a novel understanding of the historical biogeography of widespread Pacific genera that have origins in the Southern Hemisphere.
Influence of management regime and harvest date on the forage quality of rangelands plants: the importance of dry matter content
Authors: Bumb Iris , Garnier Eric , Bastianelli Denis , Richarte Jean , Bonnal Laurent , Kazakou Elena 
Published: June 2016

AbstractIn spite of their recognized ecological value, relatively little is known about the nutritional value of species-rich rangelands for herbivores. We investigated the sources of variation in dry matter digestibility (DMD), neutral detergent fibre content (NDF) and nitrogen concentration (NC) in plants from species-rich Mediterranean rangelands in southern France, and tested whether the dry matter content (DMC) was a good predictor of the forage quality of different plant parts. Sixteen plant species with contrasting growth forms (rosette, tussock, extensive and stemmed-herb) were studied, representative of two management regimes imposed in these rangelands: (i) fertilization and intensive grazing and (ii) non-fertilization and moderate grazing. Among the 16 plant species, four species were found in both treatments, allowing us to assess the intraspecific variability in forage quality and DMC across the treatments. The components of nutritional value (DMD, NDF and NC) as well as the DMC of leaves, stems and reproductive plant parts, were assessed at the beginning of the growing season and at peak standing biomass. All components of nutritional value and DMC were affected by species growth form: rosettes had higher DMD and NC than tussocks; the reverse being found for NDF and DMC. As the season progressed, DMD and NC of the different plant parts decreased while NDF and DMC increased for all species. DMC was negatively related to DMD and NC and positively to NDF, regardless of the source of variation (species, harvest date, management regime or plant part). Path analysis indicated that NDF was the main determinant of DMD. Better assessment of forage quality in species-rich systems requires consideration of their growth form composition. DMC of all plant parts, which is closely related to NDF, emerged as a good predictor and easily measured trait to estimate DMD in these species-rich systems.
SERAPHIM: studying environmental rasters and phylogenetically informed movements
Authors: Dellicour Simon , Rose Rebecca , Faria Nuno R. , Lemey Philippe , Pybus Oliver G. 
Published: June 2016

Abstract
Summary: SERAPHIM (“Studying Environmental Rasters and PHylogenetically Informed Movements”) is a suite of computational methods developed to study phylogenetic reconstructions of spatial movement in an environmental context. SERAPHIM extracts the spatio-temporal information contained in estimated phylogenetic trees and uses this information to calculate summary statistics of spatial spread and to visualize dispersal history. Most importantly, SERAPHIM enables users to study the impact of customized environmental variables on the spread of the study organism. Specifically, given an environmental raster, SERAPHIM computes environmental “weights” for each phylogeny branch, which represent the degree to which the environmental variable impedes (or facilitates) lineage movement. Correlations between movement duration and these environmental weights are then assessed, and the statistical significances of these correlations are evaluated using null distributions generated by a randomization procedure. SERAPHIM can be applied to any phylogeny whose nodes are annotated with spatial and temporal information. At present, such phylogenies are most often found in the field of emerging infectious diseases, but will become increasingly common in other biological disciplines as population genomic data grows.
Availability and Implementation: SERAPHIM 1.0 is freely available from http://evolve.zoo.ox.ac.uk/. R package, source code, example files, tutorials and a manual are also available from this website.
Contact: simon.dellicour@kuleuven.be or oliver.pybus@zoo.ox.ac.uk
Supplementary information: Supplementary data are available at Bioinformatics online.
Population structure, gene flow, and historical demography of a small coastal shark (Carcharhinus isodon) in US waters of the Western Atlantic Ocean
Authors: Portnoy David S. , Hollenbeck Christopher M. , Bethea Dana M. , Frazier Bryan S. , Gelsleichter Jim , Gold John R. 
Published: June 2016

AbstractPatterns of population structure, genetic demographics, and gene flow in the small coastal shark Carcharhinus isodon (finetooth shark) sampled from two discrete nurseries along the southeastern US coast (Atlantic) and three nurseries in the northern Gulf of Mexico (Gulf), were assessed using 16 nuclear-encoded microsatellites and 1077 base pairs of the mitochondrial DNA (mtDNA) control region. Significant heterogeneity in microsatellite allele distributions was detected among all localities except between the two in the Atlantic. Significant heterogeneity in mtDNA haplotypes was not detected, a result likely due to extremely low mtDNA diversity. The genetic discontinuities combined with seasonal movement patterns, a patchy distribution of appropriate nursery habitat, the apparent absence of sex-biased gene flow, and the occurrence of mating in the vicinity of nursery areas, suggest that both male and female finetooth sharks display regional philopatry to discrete nursery areas. Global and local tests of neutrality, using mtDNA haplotypes, and demographic model testing, using Approximate Bayesian Computation of microsatellite alleles, supported a range-wide expansion of finetooth sharks into US waters occurring less than ∼9000 years ago. These findings add to the growing number of studies in a variety of coastally distributed marine fishes documenting significant barriers to gene flow around peninsular Florida and in the eastern Gulf. The findings also provide further evidence that the traditional model of behavioural ecology, based on large coastal sharks, may not be appropriate for understanding and conserving small coastal sharks.