bluuebunny/crossref_metadata_2025_split huggingface dataset:
Necessary Conformism: An Art of Living for Young People in Turkey
Authors: Lðkðslð Demet 
Published: February 2016

AbstractThis paper focuses on the everyday life experiences of the post-1980 generation in Turkey–a generation stigmatized for being depoliticized and apathetic. Rather than accepting this stigmatizing view, however, this analysis aims to better understand young people's actual lived experiences. To do so, it adopts the concept of “necessary conformism” developed in previous empirical research. This concept offers an alternative analytical framework that transcends the engaged/disengaged or political/ unpolitical dichotomy in young people's social participation. Specifically, the application of this concept reveals that apathetic behavior may actually mask powerful discontent and suffering that can be expressed neither through conventional politics nor open resistance. The necessary conformism of young people, therefore, is not apathetic behavior, but the expression of an underlying discontent and often a hidden agony.
Globalization, modernity and democracy: In search of a viable domestic polity for a sustainable Turkish foreign policy
Authors: Keyman E. Fuat 
Published: February 2016

AbstractIn recent years. Turkey has initiated a proactive, multi-dimensional and constructive foreign policy in many areas, ranging from contributing to peace and stability in the Middle East, to playing an active role in countering terrorism and extremism, from becoming a new “energy hub” to acting as one of the architects of “the inter-civilization dialogue initiative” aiming at producing a vision of the world, based on dialogue, tolerance and living together. Thus, there has been an upsurge of interest in, and a global attraction to, Turkey and its contemporary history. Moreover, the global attraction to Turkey has stemmed not only from the geopolitical identity of Turkey, as a strong state with the capacity to function as a “geopolitical security hinge” in the intersection of the Middle East, the Balkans and the Caucasian regions, but also from its cultural identity as a modern national formation with parliamentary democratic governance, secular constitutional structure and mainly Muslim population. Furthermore, as the world has become more globalized, more interdependent and more risky, this new foreign policy identity entailed the employment of not only geopolitics but also identity and economy. Thus, geopolitics, modernity and democracy have become the constitutive dimensions of Turkish foreign policy today This paper explores the ways in which the increasing role and visibility of “soft power” in Turkish foreign policy operates, and suggests that to be sustainable, Turkish foreign policy, relying on soft power, should go hand in hand with the process of the consolidation of Turkish democracy, and also accept and put into practice Turkey-EU relations as the main axis of proactiveness and constructiveness.
Falling from grace: Gender norms and gender strategies in Eastern Turkey
Authors: Weiss Nerina 
Published: February 2016

AbstractThis article calls for a critical scholarly engagement with women's participation in the Kurdish movement. Since the 1980s, women have appropriated the political sphere in different gender roles, and their activism is mostly seen as a way of empowerment and emancipation. Albeit legitimate, such a claim often fails to account for the social and political control mechanisms inherent in the new political gender roles. This article presents the life stories of four Kurdish women. Although politically active, these women do not necessarily define themselves through their political activity. Thus they do not present their life story according to the party line, but dwell on the different social and political expectations, state violence and the contradicting role models with whom they have to deal on a daily basis. Therefore, the status associated with their roles, especially those of the “new” and emancipated woman, does not necessarily represent their own experiences and subjectivities. Women who openly criticize the social and political constraints by transgressing the boundaries of accepted conduct face social as well as political sanctions.
The Rebuilding of İstanbul Revisited: Foreign Planners in the Early Republican Years
Authors: Akpınar İpek 
Published: February 2016

AbstractIn the 1930s, the attention of Turkey’s politicians shifted back from Ankara and Anatolian cities to İstanbul. In 1932, the Governorship-Municipality of İstanbul organized an urban design competition for İstanbul, and four foreign city planners were invited. In the meantime, Martin Wagner came to İstanbul for the preparation of urban reports. In 1937, Henri Prost, the prominent urbanist of Paris, was invited to İstanbul and prepared the first master plan of the city. In Turkey and in İstanbul, town planning processes have been significantly influenced by “Western” planning principles, cultures, and experiences while gaining a local meaning in the context of Turkish politics and the state-formation process. The aim of this study is to describe the urban design competition of 1933 and the first master plan of 1937. Beyond references to Western European cities as in the “city-beautiful” planning approach, this study, based on a series of official documents, plan reports and their rhetoric, investigates in particular the role of foreign planners/urbanists in İstanbul in the context of the construction of a nation-state. The analysis of these foreign planners’ work suggests that urban planning in Republican Turkey was closely linked to the construction of the nation state.
Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour
Authors: Doi Masao , Murai Iori , Kunisue Sumihiro , Setsu Genzui , Uchio Naohiro , Tanaka Rina , Kobayashi Sakurako , Shimatani Hiroyuki , Hayashi Hida , Chao Hsu-Wen , Nakagawa Yuuki , Takahashi Yukari , Hotta Yunhong , Yasunaga Jun-ichirou , Matsuoka Masao , Hastings Michael H. , Kiyonari Hiroshi , Okamura Hitoshi 
Published: February 2016

AbstractG-protein-coupled receptors (GPCRs) participate in a broad range of physiological functions. A priority for fundamental and clinical research, therefore, is to decipher the function of over 140 remaining orphan GPCRs. The suprachiasmatic nucleus (SCN), the brain’s circadian pacemaker, governs daily rhythms in behaviour and physiology. Here we launch the SCN orphan GPCR project to (i) search for murine orphan GPCRs with enriched expression in the SCN, (ii) generate mutant animals deficient in candidate GPCRs, and (iii) analyse the impact on circadian rhythms. We thereby identify Gpr176 as an SCN-enriched orphan GPCR that sets the pace of circadian behaviour. Gpr176 is expressed in a circadian manner by SCN neurons, and molecular characterization reveals that it represses cAMP signalling in an agonist-independent manner. Gpr176 acts independently of, and in parallel to, the Vipr2 GPCR, not through the canonical Gi, but via the unique G-protein subclass Gz.
Luminescent platinum(II) complexes with functionalized N-heterocyclic carbene or diphosphine selectively probe mismatched and abasic DNA
Authors: Fung Sin Ki , Zou Taotao , Cao Bei , Chen Tianfeng , To Wai-Pong , Yang Chen , Lok Chun-Nam , Che Chi-Ming 
Published: February 2016

AbstractThe selective targeting of mismatched DNA overexpressed in cancer cells is an appealing strategy in designing cancer diagnosis and therapy protocols. Few luminescent probes that specifically detect intracellular mismatched DNA have been reported. Here we used Pt(II) complexes with luminescence sensitive to subtle changes in the local environment and report several Pt(II) complexes that selectively bind to and identify DNA mismatches. We evaluated the complexes’ DNA-binding characteristics by ultraviolet/visible absorption titration, isothermal titration calorimetry, nuclear magnetic resonance and quantum mechanics/molecular mechanics calculations. These Pt(II) complexes show up to 15-fold higher emission intensities upon binding to mismatched DNA over matched DNA and can be utilized for both detecting DNA abasic sites and identifying cancer cells and human tissue samples with different levels of mismatch repair. Our work highlights the potential of luminescent Pt(II) complexes to differentiate between normal cells and cancer cells which generally possess more aberrant DNA structures.
Nanotwin-governed toughening mechanism in hierarchically structured biological materials
Authors: Shin Yoon Ah , Yin Sheng , Li Xiaoyan , Lee Subin , Moon Sungmin , Jeong Jiwon , Kwon Minhyug , Yoo Seung Jo , Kim Young-Min , Zhang Teng , Gao Huajian , Oh Sang Ho 
Published: February 2016

AbstractAs a natural biocomposite, Strombus gigas, commonly known as the giant pink queen conch shell, exhibits outstanding mechanical properties, especially a high fracture toughness. It is known that the basic building block of conch shell contains a high density of growth twins with average thickness of several nanometres, but their effects on the mechanical properties of the shell remain mysterious. Here we reveal a toughening mechanism governed by nanoscale twins in the conch shell. A combination of in situ fracture experiments inside a transmission electron microscope, large-scale atomistic simulations and finite element modelling show that the twin boundaries can effectively block crack propagation by inducing phase transformation and delocalization of deformation around the crack tip. This mechanism leads to an increase in fracture energy of the basic building block by one order of magnitude, and contributes significantly to that of the overall structure via structural hierarchy.
A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix
Authors: Liang Hui , Li Xiaoran , Wang Bin , Chen Bing , Zhao Yannan , Sun Jie , Zhuang Yan , Shi Jiajia , Shen He , Zhang Zhijun , Dai Jianwu 
Published: February 2016

AbstractMany tumors over-express collagen, which constitutes the physical scaffold of tumor microenvironment. Collagen has been considered to be a target for cancer therapy. The collagen-binding domain (CBD) is a short peptide, which could bind to collagen and achieve the sustained release of CBD-fused proteins in collagen scaffold. Here, a collagen-binding EGFR antibody fragment was designed and expressed for targeting the collagen-rich extracellular matrix in tumors. The antibody fragment (Fab) of cetuximab was fused with CBD (CBD-Fab) and expressed in Pichia pastoris. CBD-Fab maintained antigen binding and anti-tumor activity of cetuximab and obtained a collagen-binding ability in vitro. The results also showed CBD-Fab was mainly enriched in tumors and had longer retention time in tumors in A431 s.c. xenografts. Furthermore, CBD-Fab showed a similar therapeutic efficacy as cetuximab in A431 xenografts. Although CBD-Fab hasn’t showed better therapeutic effects than cetuximab, its smaller molecular and special target may be applicable as antibody–drug conjugates (ADC) or immunotoxins.
Enrichment and isolation of Flavobacterium strains with tolerance to high concentrations of cesium ion
Authors: Kato Souichiro , Goya Eri , Tanaka Michiko , Kitagawa Wataru , Kikuchi Yoshitomo , Asano Kozo , Kamagata Yoichi 
Published: February 2016

AbstractInterest in the interaction of microorganisms with cesium ions (Cs+) has arisen, especially in terms of their potent ability for radiocesium bioaccumulation and their important roles in biogeochemical cycling. Although high concentrations of Cs+ display toxic effects on microorganisms, there have been only limited reports for Cs+-tolerant microorganisms. Here we report enrichment and isolation of Cs+-tolerant microorganisms from soil microbiota. Microbial community analysis revealed that bacteria within the phylum Bacteroidetes, especially Flavobacterium spp., dominated in enrichment cultures in the medium supplemented with 50 or 200 mM Cs+, while Gammaproteobacteria was dominant in the control enrichment cultures (in the presence of 50 and 200 mM K+ instead of Cs+). The dominant Flavobacterium sp. was successfully isolated from the enrichment culture and was closely related to Flavobacterium chungbukense with 99.5% identity. Growth experiments clearly demonstrated that the isolate has significantly higher tolerance to Cs+ compared to its close relatives, suggesting the Cs+-tolerance is a specific trait of this strain, but not a universal trait in the genus Flavobacterium. Measurement of intracellular K+ and Cs+ concentrations of the Cs+-tolerant isolate and its close relatives suggested that the ability to maintain low intracellular Cs+ concentration confers the tolerance against high concentrations of external Cs+.
Novel Vertical 3D Structure of TaOx-based RRAM with Self-localized Switching Region by Sidewall Electrode Oxidation
Authors: Yu Muxi , Cai Yimao , Wang Zongwei , Fang Yichen , Liu Yefan , Yu Zhizhen , Pan Yue , Zhang Zhenxing , Tan Jing , Yang Xue , Li Ming , Huang Ru 
Published: February 2016

AbstractA novel vertical 3D RRAM structure with greatly improved reliability behavior is proposed and experimentally demonstrated through basically compatible process featuring self-localized switching region by sidewall electrode oxidation. Compared with the conventional structure, due to the effective confinement of the switching region, the newly-proposed structure shows about two orders higher endurance (>108 without verification operation) and better retention (>180h@150 °C), as well as high uniformity. Corresponding model is put forward, on the base of which thorough theoretical analysis and calculations are conducted as well, demonstrating that, resulting from the physically-isolated switching from neighboring cells, the proposed structure exhibits dramatically improved reliability due to effective suppression of thermal effects and oxygen vacancies diffusion interference, indicating that this novel structure is very promising for future high density 3D RRAM application.
Growth of Continuous Monolayer Graphene with Millimeter-sized Domains Using Industrially Safe Conditions
Authors: Wu Xingyi , Zhong Guofang , D'Arsié Lorenzo , Sugime Hisashi , Esconjauregui Santiago , Robertson Alex W. , Robertson John 
Published: February 2016

AbstractWe demonstrate the growth of continuous monolayer graphene films with millimeter-sized domains on Cu foils under intrinsically safe, atmospheric pressure growth conditions, suitable for application in roll-to-roll reactors. Previous attempts to grow large domains in graphene have been limited to isolated graphene single crystals rather than as part of an industrially useable continuous film. With both appropriate pre-treatment of the Cu and optimization of the CH4 supply, we show that it is possible to grow continuous films of monolayer graphene with millimeter scale domains within 80 min by chemical vapour deposition. The films are grown under industrially safe conditions, i.e., the flammable gases (H2 and CH4) are diluted to well below their lower explosive limit. The high quality, spatial uniformity, and low density of domain boundaries are demonstrated by charge carrier mobility measurements, scanning electron microscope, electron diffraction study, and Raman mapping. The hole mobility reaches as high as ~5,700 cm2 V−1 s−1 in ambient conditions. The growth process of such high-quality graphene with a low H2 concentration and short growth times widens the possibility of industrial mass production.
Peptide Reactivity of Isothiocyanates – Implications for Skin Allergy
Authors: Karlsson Isabella , Samuelsson Kristin , Ponting David J. , Törnqvist Margareta , Ilag Leopold L. , Nilsson Ulrika 
Published: February 2016

AbstractSkin allergy is a chronic condition that affects about 20% of the population of the western world. This disease is caused by small reactive compounds, haptens, able to penetrate into the epidermis and modify endogenous proteins, thereby triggering an immunogenic reaction. Phenyl isothiocyanate (PITC) and ethyl isothiocyanate (EITC) have been suggested to be responsible for allergic skin reactions to chloroprene rubber, the main constituent of wetsuits, orthopedic braces, and many types of sports gear. In the present work we have studied the reactivity of the isothiocyanates PITC, EITC, and tetramethylrhodamine-6-isothiocyanate (6-TRITC) toward peptides under aqueous conditions at physiological pH to gain information about the types of immunogenic complexes these compounds may form in the skin. We found that all three compounds reacted quickly with cysteine moieties. For PITC and 6-TRITC the cysteine adducts decomposed over time, while stable adducts with lysine were formed. These experimental findings were verified by DFT calculations. Our results may suggest that the latter are responsible for allergic reactions to isothiocyanates. The initial adduct formation with cysteine residues may still be of great importance as it prevents hydrolysis and facilitates the transport of isothiocyanates into epidermis where they can form stable immunogenic complexes with lysine-containing proteins.
Tissue-based multiphoton analysis of actomyosin and structural responses in human trabecular meshwork
Authors: Gonzalez Jose M. , Ko Minhee K. , Pouw Andrew , Tan James C. H. 
Published: February 2016

AbstractThe contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary goal was to ascertain if structural extracellular matrix (ECM) configuration changed with contractility. Viable ex vivo human TM was incubated with latrunculin-A (Lat-A) or vehicle prior to Alexa-568-phalloidin labeling and TPEF. Quantitative image analysis was applied to 2-dimensional (2D) optical sections and 3D image reconstructions. After Lat-A exposure, (a) the F-actin network reorganized as aggregates; (b) F-actin-associated fluorescence intensity was reduced by 48.6% (mean; p = 0.007; n = 8); (c) F-actin 3D distribution was reduced by 68.9% (p = 0.040); (d) ECM pore cross-sectional area and volume were larger by 36% (p = 0.032) and 65% (p = 0.059) respectively and pores appeared more interconnected; (e) expression of type I collagen and elastin, key TM structural ECM proteins, were unaltered (p = 0.54); and (f) tissue viability was unchanged (p = 0.39) relative to vehicle controls. Thus Lat-A-induced reduction of actomyosin contractility was associated with TM porous expansion without evidence of reduced structural ECM protein expression or cellular viability. These important subcellular-level dynamics could be visualized and quantified within human tissue by TPEF.
A novel gene delivery composite system based on biodegradable folate-poly (ester amine) polymer and thermosensitive hydrogel for sustained gene release
Authors: Yang Yi , Zhao Hang , Jia YanPeng , Guo QingFa , Qu Ying , Su Jing , Lu XiaoLing , Zhao YongXiang , Qian ZhiYong 
Published: February 2016

AbstractLocal anti-oncogene delivery providing high local concentration of gene, increasing antitumor effect and decreasing systemic side effects is currently attracting interest in cancer therapy. In this paper, a novel local sustained anti-oncogene delivery system, PECE thermoresponsive hydrogel containing folate-poly (ester amine) (FA-PEA) polymer/DNA (tumor suppressor) complexes, is demonstrated. First, a tumor-targeted biodegradable folate-poly (ester amine) (FA-PEA) polymer based on low-molecular-weight polyethyleneimine (PEI) was synthesized and characterized, and the application for targeted gene delivery was investigated. The polymer had slight cytotoxicity and high transfection efficiency in vitro compared with PEI 25k, which indicated that FA-PEA was a potential vector for targeted gene delivery. Meanwhile, we successfully prepared a thermoresponsive PECE hydrogel composite containing FA-PEA/DNA complexes which could contain the genes and slowly release the genes into cells. We concluded the folate-poly (ester amine) (FA-PEA) polymer would be useful for targeted gene delivery, and the novel gene delivery composite based on biodegradable folate-poly (ester amine) polymer and thermosensitive PECE hydrogel showed potential for sustained gene release.
Tracking the immunopathological response to Pseudomonas aeruginosa during respiratory infections
Authors: Cigana Cristina , Lorè Nicola Ivan , Riva Camilla , De Fino Ida , Spagnuolo Lorenza , Sipione Barbara , Rossi Giacomo , Nonis Alessandro , Cabrini Giulio , Bragonzi Alessandra 
Published: February 2016

AbstractRepeated cycles of infections, caused mainly by Pseudomonas aeruginosa, combined with a robust host immune response and tissue injury, determine the course and outcome of cystic fibrosis (CF) lung disease. As the disease progresses, P. aeruginosa adapts to the host modifying dramatically its phenotype; however, it remains unclear whether and how bacterial adaptive variants and their persistence influence the pathogenesis and disease development. Using in vitro and murine models of infection, we showed that P. aeruginosa CF-adaptive variants shaped the innate immune response favoring their persistence. Next, we refined a murine model of chronic pneumonia extending P. aeruginosa infection up to three months. In this model, including CFTR-deficient mice, we unveil that the P. aeruginosa persistence lead to CF hallmarks of airway remodelling and fibrosis, including epithelial hyperplasia and structure degeneration, goblet cell metaplasia, collagen deposition, elastin degradation and several additional markers of tissue damage. This murine model of P. aeruginosa chronic infection, reproducing CF lung pathology, will be instrumental to identify novel molecular targets and test newly tailored molecules inhibiting chronic inflammation and tissue damage processes in pre-clinical studies.
An in-situ synthesis of Ag/AgCl/TiO2/hierarchical porous magnesian material and its photocatalytic performance
Authors: Yang Lu , Wang Fazhou , Shu Chang , Liu Peng , Zhang Wenqin , Hu Shuguang 
Published: February 2016

AbstractThe absorption ability and photocatalytic activity of photocatalytic materials play important roles in improving the pollutants removal effects. Herein, we reported a new kind of photocatalytic material, which was synthesized by simultaneously designing hierarchical porous magnesian (PM) substrate and TiO2 catalyst modification. Particularly, PM substrate could be facilely prepared by controlling its crystal phase (Phase 5, Mg3Cl(OH)5·4H2O), while Ag/AgCl particles modification of TiO2 could be achieved by in situ ion exchange between Ag+ and above crystal Phase. Physiochemical analysis shows that Ag/AgCl/TiO2/PM material has higher visible and ultraviolet light absorption response and excellent gas absorption performance compared to other controls. These suggested that Ag/AgCl/TiO2/PM material could produce more efficient photocatalytic effects. Its photocatalytic reaction rate was 5.21 and 30.57 times higher than that of TiO2/PM and TiO2/imporous magnesian substrate, respectively. Thus, this material and its intergration synthesis method could provide a novel strategy for high-efficiency application and modification of TiO2 photocatalyst in engineering filed.