bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Total Chemical Synthesis of 13C=18O Isotopomers of Human Insulin for Isotope‐Edited FTIR
Authors: Dhayalan Balamurugan , Fitzpatrick Ann , Mandal Kalyaneswar , Whittaker Jonathan , Weiss Michael A. , Tokmakoff Andrei , Kent Stephen B. H. 
Published: December 2015

AbstractIsotope‐edited two‐dimensional Fourier transform infrared spectroscopy (2 D FTIR) can potentially provide a unique probe of protein structure and dynamics. However, general methods for the site‐specific incorporation of stable 13C=18O labels into the polypeptide backbone of the protein molecule have not yet been established. Here we describe, as a prototype for the incorporation of specific arrays of isotope labels, the total chemical synthesis—via a key ester insulin intermediate—of 97 % enriched [(1‐13C=18O)PheB24] human insulin: stable‐isotope labeled at a single backbone amide carbonyl. The amino acid sequence as well as the positions of the disulfide bonds and the correctly folded structure were unambiguously confirmed by the X‐ray crystal structure of the synthetic protein molecule. In vitro assays of the isotope labeled [(1‐13C=18O)PheB24] human insulin showed that it had full insulin receptor binding activity. Linear and 2 D IR spectra revealed a distinct red‐shifted amide I carbonyl band peak at 1595 cm−1 resulting from the (1‐13C=18O)PheB24 backbone label. This work illustrates the utility of chemical synthesis to enable the application of advanced physical methods for the elucidation of the molecular basis of protein function.
Primary‐like human hepatocytes genetically engineered to obtain proliferation competence display hepatic differentiation characteristics in monolayer and organotypical spheroid cultures
Authors: Herzog Natalie , Hansen Max , Miethbauer Sebastian , Schmidtke Kai‐Uwe , Anderer Ursula , Lupp Amelie , Sperling Sebastian , Seehofer Daniel , Damm Georg , Scheibner Katrin , Küpper Jan‐Heiner 
Published: December 2015

AbstractPrimary human hepatocytes are in great demand during drug development and in hepatology. However, both scarcity of tissue supply and donor variability of primary cells create a need for the development of alternative hepatocyte systems. By using a lentivirus vector system to transfer coding sequences of Upcyte® proliferation genes, we generated non‐transformed stable hepatocyte cultures from human liver tissue samples. Here, we show data on newly generated proliferation‐competent HepaFH3 cells investigated as conventional two‐dimensional monolayer and as organotypical three‐dimensional (3D) spheroid culture. In monolayer culture, HepaFH3 cells show typical cobblestone‐like hepatocyte morphology and anchorage‐dependent growth for at least 20 passages. Immunofluorescence staining revealed that characteristic hepatocyte marker proteins cytokeratin 8, human serum albumin, and cytochrome P450 (CYP) 3A4 were expressed. Quantitative real‐time PCR analyses showed that expression levels of analyzed phase I CYP enzymes were at similar levels compared to those of cultured primary human hepatocytes and considerably higher than in the liver carcinoma cell line HepG2. Additionally, transcripts for phase II liver enzymes and transporter proteins OATP‐C, MRP2, Oct1, and BSEP were present in HepaFH3. The cells produced urea and converted model compounds such as testosterone, diclofenac, and 7‐OH‐coumarin into phases I and II metabolites. Interestingly, phases I and II enzymes were expressed at about the same levels in convenient monolayer cultures and complex 3D spheroids. In conclusion, HepaFH3 cells and related primary‐like hepatocyte lines seem to be promising tools for in vitro research of liver functions and as test system in drug development and toxicology analysis.
Catheter interventional treatment of congenital portosystemic venous shunts in childhood
Authors: Knirsch Walter , Benz Dominik C. , Bühr Patrick , Quandt Daniel , Weber Roland , Kellenberger Christian , Braegger Christian P. , Kretschmar Oliver 
Published: December 2015

ObjectivesThe study aimed on the catheter interventional treatment of congenital portosystemic venous shunt (CPSVS) in childhood and the impact on vascular growth of hypoplastic portal veins. Clinical course and follow up of partial or complete closure of CPSVS are described.BackgroundCPSVS is a very rare vascular malformation of the portal venous drainage resulting in potentially life threatening abdominal, pulmonary, cerebral, and cardiac complications. The anatomic type, clinical course, and diagnosis must be determined for optimal management.MethodsSingle centre case series.ResultsEight (6 female) children with extrahepatic (n = 5) and intrahepatic (n = 3) CPSVS were diagnosed invasively by catheterization, including test balloon occlusion of the shunt and simultaneous retrograde angiography, and treated by catheter interventions (n = 5) with partial (n = 2) and complete (n = 3) occlusion of CPSVS at a median age of 3.9 years (range 0.7–21). Congenital heart disease (CHD) was the most frequent associated organ manifestation (n = 5) followed by mild to severe pulmonary arterial hypertension (n = 4), hepatopulmonary syndrome (n = 2), and portosystemic encephalopathy (n = 1). CHD was simple (n = 3) or complex type (n = 2). Three patients were untreated so far, because they were in excellent clinical condition at an age <1 year, refused treatment, or planned for later treatment.ConclusionsAccurate invasive diagnosis of CPSVS with test balloon occlusion of the shunt is mandatory to depict the anatomic situation. Catheter interventional treatment of CPSVS offers a feasible and safe approach with complete or partial closure of the vascular malformation inducing potentially significant vascular growth of a former hypoplastic portal venous system. © 2015 Wiley Periodicals, Inc.
Treatment of drug‐eluting stent restenosis: Comparison between drug‐eluting balloon versus second‐generation drug‐eluting stents from a retrospective observational study
Authors: Basavarajaiah Sandeep , Naganuma Toru , Latib Azeem , Sticchi Alessandro , Ciconte Giuseppe , Panoulas Vasilis , Chieffo Alaide , Montorfano Matteo , Carlino Mauro , Colombo Antonio 
Published: December 2015

ObjectivesTo compare drug‐eluting balloons (DEBs) versus second‐generation DES in the treatment of drug‐eluting stent restenosis (DES‐ISR).BackgroundThe optimal treatment of DES‐ISR remains unclear. Several modes of treatment ranging from plain‐old balloon angioplasty to repeated use of DES have yielded disappointing results. DEBs are increasingly been used in restenotic lesions; however, their use in DES‐ISR is less established.MethodsWe evaluated all procedures between 2009 and 2011, involving DES‐ISR that were treated either with DEB or second‐generation DES. The measured end‐points during the follow‐up period were cardiac‐death, target‐vessel MI, TLR, TVR, and MACE defined as composite of cardiac‐death, TV‐MI, and TVR.ResultsTwo hundred and forty‐seven patients (302 lesions) with DES‐ISR were treated with either DEB (81 patients; 104 lesions) or second‐generation DES (166 patients; 198 lesions). The mean age of patients was 66.1 ± 9.4 years. There were higher numbers of patients with diabetes in the DEB group (DEB 47% vs DES 33%; p = 0.03). The mean length of DEB was significantly longer than the DES (35.4 vs 19.8 mm; p < 0.001). During the 12‐month follow‐up, there were no significant differences in the MACE rates (12.3% vs 8.4%; p = 0.3) and TLR rates (9.9% vs 7.8%; p = 0.6) between DEB and DES, respectively. On the multivariate analysis, use of DEB or DES was not the predictor of MACE (hazard ratio: 0.84, 95% CI: 0.46–1.85; p = 0.6). There were no cases of definite or probable stent thrombosis in either group.ConclusionThere were no significant differences in the clinical outcomes between DEB and second‐generation DES in the treatment of DES‐ISR. These results should encourage operators to consider DEB in the treatment of DES‐ISR, which offers certain advantages over DES. © 2015 Wiley Periodicals, Inc.
Incidence, treatment, and outcome of acute aortic valve regurgitation complicating percutaneous balloon aortic valvuloplasty
Authors: Dall'Ara Gianni , Saia Francesco , Moretti Carolina , Marrozzini Cinzia , Taglieri Nevio , Bordoni Barbara , Chiarabelli Matteo , Ciuca Cristina , Rapezzi Claudio , Marzocchi Antonio 
Published: December 2015

ObjectivesTo evaluate the incidence, treatment, and outcomes of acute aortic regurgitation (ARR) complicating BAV.BackgroundIn the transcatheter aortic valve implantation (TAVI) era, there is an increase of percutaneous balloon aortic valvuloplasty (BAV) procedures with different indications.MethodsFrom the prospective BAV registry of the University of Bologna, which has enrolled patients between the year 2000 and the present, we selected those who suffered intraprocedural AAR with overt hemodynamic instability. Worsening of baseline aortic insufficiency without hemodynamic collapse, treatment of degenerated biological valve prosthesis, and BAV performed within a planned TAVI procedure were excluded. The main endpoints were in‐hospital and 30‐day mortality.ResultsOut of 1517 BAVs, we identified 26 cases of AAR (1.7%). This complication occurred in 80.8% of cases after one or two balloon inflations. Mean transaortic gradient decreased from 50.6 ± 19.3 to 26.0 ± 14.4 mm Hg (p < 0.01). In 8(30.8%) patients, AAR spontaneously resolved within few minutes; in 18 cases, the operators had to perform a rescue maneuver to reposition a valve leaflet got stuck in the opening position (this maneuver was successful in 13/18 of the cases, 72.2%). Out of 5 persistent AAR, 3 were managed with emergency TAVI or surgery, while 2 were unresolved. In‐hospital mortality was 15.4% (n = 4), whereas no more deaths occurred up to 30 days.ConclusionsAAR is a fearsome complication of BAV and portends a grim prognosis. In some cases, it can be resolved with appropriate technical maneuvers; in others, a rescue TAVI or surgical valve replacement may be necessary. © 2015 Wiley Periodicals, Inc.
The Origin of Catalytic Activity of Nickel Phosphate for Oxygen Evolution in Alkaline Solution and its Further Enhancement by Iron Substitution
Authors: Zhan Yi , Lu Meihua , Yang Shiliu , Liu Zhaolin , Lee Jim Yang 
Published: December 2015

AbstractNickel(II) phosphate (NPO) can be an effective catalyst for oxygen evolution reaction (OER) in alkaline solution. Unlike other transition metal phosphates, the OER is preceded by a prominent reversible redox reaction. The current study is aimed at understanding the origin of NPO‐catalyzed OER, in particular the role of the pre‐OER redox reaction. We detected the progressive formation of Ni(OH)2/NiOOH on the NPO surface during potential cycling in the anodic region similar to previous observations from Ni oxides, and found that it is associated with the change in the Ni/phosphate stoichiometry from 1.5 to 1.0, resulting in the loss of phosphate to the alkaline solution. Despite the conversion, NPO still has a good and sustained OER performance due to the activity similarity between NiPO4 (and possibly NPO) and NiOOH (the activated form of Ni oxide and hydroxide catalysts). NPO is however an easier catalyst to use than Ni oxides and hydroxides, which require extensive electrode conditioning to reach a stable performance. We also mixed NPO with Fe in view of recent findings that Ni–Fe double hydroxides are more active than simple Ni oxides in oxygen catalysis. The experimental results corroborate the positive outcome of this modification.
Vanadium‐Ion Redox Reactions in a Three‐Dimensional Network of Reduced Graphite Oxide
Authors: Maruyama Jun , Shinagawa Tsutomu , Hayashida Akihiro , Matsuo Yoshiaki , Nishihara Hirotomo , Kyotani Takashi 
Published: December 2015

AbstractA three‐dimensional (3D) fine network structure of thin carbon microflakes inside a loosely entangled carbon‐fiber matrix was formed by applying an ice‐template method to a graphite oxide (GO) dispersion in the matrix and thermal reduction of the GO. Vanadium‐ion redox reactions were investigated in the network of reduced GO (RGO) for potential application as electrodes in vanadium redox flow batteries (VRFB). The development of the fineness, the degree of graphitization, the structural disorder, and the surface composition were characterized. The interaction between the carbon surface species and the vanadium ions was implied by the X‐ray absorption fine structure (XAFS). The structure of the 3D RGO network was clearly correlated to the activity for the VRFB positive electrode reactions, and the development of the fine network structure effectively enhanced the reactions due to increased exposure of the edge planes and sufficient dispersion of the active sites.
A Preliminary Study of Diastereoselectivity in the PdII‐Catalyzed C(sp3)‐H Alkoxylation of Cyclic Systems
Authors: Yang Xinglin , Sun Tian‐Yu , Rao Yu 
Published: December 2015

AbstractPrimary mechanism of a PdII‐catalyzed 8‐aminoquinoline‐directed C−H alkoxylation was investigated. It was understood that the PdII‐catalyzed C(sp3)−O bond formation proceeded through a concerted reductive elimination from the PdIV intermediate in the cyclic system. Deuteration experiments and related computational studies elucidate that intrinsic conformation determined the diastereoselectivity of the PdII‐catalyzed C−H alkoxylation of cyclic carboxylic acids.
Baseline dietary glutamic acid intake and the risk of colorectal cancer: The Rotterdam study
Authors: Viana Veloso Gilson G. , Franco Oscar H. , Ruiter Rikje , de Keyser Catherina E. , Hofman Albert , Stricker Bruno C. , Kiefte‐de Jong Jessica C. 
Published: December 2015

BACKGROUNDAnimal studies have shown that glutamine supplementation may decrease colon carcinogenesis, but any relation with glutamine or its precursors has not been studied in humans. The primary aim of this study was to assess whether dietary glutamic acid intake was associated with colorectal cancer (CRC) risk in community‐dwelling adults. A secondary aim was to evaluate whether the association could be modified by the body mass index (BMI).METHODSThis study was embedded in the Rotterdam study, which included a prospective cohort from 1990 onward that consisted of 5362 subjects who were 55 years old or older and were free of CRC at the baseline. Glutamic acid was calculated as a percentage of the total protein intake with a validated food frequency questionnaire at the baseline. Incident cases of CRC were pathology‐based.RESULTSDuring follow‐up, 242 subjects developed CRC. Baseline dietary glutamic acid intake was significantly associated with a lower risk of developing CRC (hazard ratio [HR] per percent increase in glutamic acid of protein, 0.78; 95% confidence interval [CI], 0.62‐0.99). After stratification for BMI, the risk reduction for CRC by dietary glutamic acid was 42% for participants with a BMI ≤ 25 kg/m2 (HR per percent increase in glutamic acid of protein, 0.58; 95% CI, 0.40‐0.85), whereas no association was found in participants with a BMI > 25 kg/m2 (HR per percent increase in glutamic acid of protein, 0.97; 95% CI, 0.73‐1.31).CONCLUSIONSOur data suggest that baseline dietary glutamic acid intake is associated with a lower risk of developing CRC, but this association may be mainly present in nonoverweight subjects. Cancer 2016;122:899–907. © 2015 American Cancer Society.
Methane Activation Mediated by a Series of Cerium–Vanadium Bimetallic Oxide Cluster Cations: Tuning Reactivity by Doping
Authors: Ma Jia‐Bi , Meng Jing‐Heng , He Sheng‐Gui 
Published: December 2015

AbstractThe reactions of cerium–vanadium cluster cations CexVyOz+ with CH4 are investigated by time‐of‐flight mass spectrometry and density functional theory calculations. (CeO2)m(V2O5)n+ clusters (m=1,2, n=1–5; m=3, n=1–4) with dimensions up to nanosize can abstract one hydrogen atom from CH4. The theoretical study indicates that there are two types of active species in (CeO2)m(V2O5)n+, V[(Ot)2]. and [(Ob)2CeOt]. (Ot and Ob represent terminal and bridging oxygen atoms, respectively); the former is less reactive than the latter. The experimentally observed size‐dependent reactivities can be rationalized by considering the different active species and mechanisms. Interestingly, the reactivity of the (CeO2)m(V2O5)n+ clusters falls between those of (CeO2)2–4+ and (V2O5)1–5+ in terms of C−H bond activation, thus the nature of the active species and the cluster reactivity can be effectively tuned by doping.
Recurrent giant mandibular ameloblastoma in young adults
Authors: Gravvanis Andreas , Koumoullis Harry D. , Anterriotis Dimitrios , Tsoutsos Dimosthenis , Katsikeris Nick 
Published: December 2015

AbstractBackgroundThe purpose of the study was to define the most appropriate management of the giant mandibular ameloblastoma (GMA) in young adults.MethodsA retrospective study was performed on patients with GMA <30 years old. The data collected included initial treatment, tumor margins, reconstruction, and follow‐up. Patients evaluated speech, chewing, swallowing, and facial appearance after definitive treatment.ResultsThirteen patients were identified with recurrent solid/multicystic disease requiring further treatment. Definitive treatment involved segmental mandibulectomy and reconstruction with free fibular flap in all patients. Seven patients had immediate reconstruction (group A) and 6 had secondary (group B). Mandibular resection was planned at least 2 cm beyond the radiological limit, free margins were achieved in all patients, and all flaps were transplanted successfully. In group A, functional score was 13.7 ± 0.45 and facial appearance score was 4.5 ± 0.49, whereas in group B were 11.16 ± 0.37 and 3.3 ± 0.5, respectively (both p < .05).ConclusionAggressive resection of the GMA and immediate reconstruction is strongly advised. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1947–E1954, 2016
Guest Editors’ Introduction: The Role of Human Resources and Organizational Factors in Ambidexterity
Authors: Junni Paulina , Sarala Riikka M. , Tarba Shlomo Y. , Liu Yipeng , Cooper Cary L. 
Published: December 2015

AbstractAmbidexterity is a growing field of management research. However, the role of human resources (HR) and organizational factors needs further exploration because of the fragmented nature of prior work and the subsequent lack of a unifying framework. Our review of 41 empirical studies identifies distinct research streams that relate to the effects of employee characteristics, leader characteristics, organizational structure, culture, social relationships, and organizational environment on ambidexterity. We discuss the most important findings within each stream, which contributes to the HR and ambidexterity literature by addressing the current state of our knowledge. To move forward research in this area, we identify important, yet underexplored areas in each stream. This contributes to the literature by highlighting specific gaps in our current knowledge that represent new avenues for future research. We also identify important interrelationships between different streams that need further clarification. We summarize our findings into an integrative model that elucidates the role of HR and organizational factors in ambidexterity. This contributes to a more comprehensive understanding of ambidexterity from the HR and organizational perspectives. © 2015 Wiley Periodicals, Inc.
FROM ME TO US: THE CONSTRUCTION OF FAMILY ALLIANCE
Authors: Galdiolo Sarah , Roskam Isabelle 
Published: December 2015

ABSTRACTThis longitudinal prospective and multi‐informant study based on a three‐wave research program (pregnancy, 12 months’ postpartum, and 16 months’ postpartum) aimed to determine the process of construction of family alliance, as assessed by the Lausanne Trilogue Play (Fivaz‐Depeursinge & Corboz‐Warnery, 1999). A model using parents’ individual characteristics (i.e., personality traits and attachment orientations) as distal variables, coparenting as a mediator, child's temperament as a moderator, and family alliance as outcome was tested using structural equation modeling on 62 nonreferred families. Results showed that both parents’ conscientiousness was positively and mothers’ avoidant attachment and fathers’ anxious attachment were negatively and indirectly (via coparenting) associated with the family alliance. The discussion underlines mothers’ and fathers’ different roles and the importance of coparenting as a core mechanism in the development of family alliance.
WHEN MUM IS STRESSED, IS DAD JUST AS STRESSED? PREDICTORS OF PATERNAL STRESS IN THE FIRST SIX MONTHS OF HAVING A BABY
Authors: Seah Cheryl K.F. , Morawska Alina 
Published: December 2015

ABSTRACTThe primary aim of this study was to examine predictors of paternal stress within the first 6 months of having a baby in a normative Australian sample, and to compare paternal and maternal stress. In total, 54 fathers and 71 mothers completed self‐report measures of postnatal depressive symptoms, parenting stress, efficacy, responsiveness, attachment, and family and social support. Paired sample t test revealed a significant relationship within couples in their level of parenting stress. Fathers reported lower levels of postnatal depression, responsiveness, and attachment than did mothers. Fathers rated their level of social support to be higher than did mothers. Regression analyses indicated that responsiveness and efficacy significantly predicted paternal stress whereas responsiveness and attachment predicted maternal stress when history of psychological diagnosis and financial stability were controlled for. Results have implications for early preventive parenting programs.
MATERNAL MIND‐MINDEDNESS: RELATIONS WITH MATERNAL–FETAL ATTACHMENT AND STABILITY IN THE FIRST TWO YEARS OF LIFE: FINDINGS FROM AN AUSTRALIAN PROSPECTIVE STUDY
Authors: McMahon Catherine , Camberis Anna‐Lisa , Berry Sinead , Gibson Frances 
Published: December 2015

ABSTRACTMind‐mindedness captures a caregiver's attunement to his or her infant's mental states, and the tendency to interpret behavior as resulting from these mental states. The construct is assessed through analysis of maternal language during interaction or from mothers’ use of mental state words when invited to describe their child. This study examined whether maternal–fetal attachment predicted maternal mind‐mindedness, whether there was continuity in mind‐mindedness over the first 2 postnatal years, and concordance for the two approaches to measurement. One hundred fifty women completed a questionnaire measure of maternal–fetal attachment in the third trimester of pregnancy and participated in home visits to assess maternal mind‐mindedness when their infants were 7 months and 19 months of age. Path analysis showed that maternal–fetal attachment predicted indices of maternal mind‐mindedness at 7 and 19 months; mothers who made more mind‐related comments during play at 7 months also did so at 19 months, and mothers who made more mind‐related comments during play at 19 months also used more mental state words when describing their child. Results suggest that a proclivity to mind‐mindedness may be a caregiver characteristic that is present prior to birth and stable over time.