bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization of gliomas and glioneuronal tumors amid Noonan syndrome: cancer predisposition examined
Authors: Shatara Margaret , Schieffer Kathleen M. , Melas Marilena , Varga Elizabeth A. , Thomas Diana , Bucknor Brianna A. , Costello Heather M. , Wheeler Gregory , Kelly Benjamin J. , Miller Katherine E. , Rodriguez Diana P. , Mathew Mariam T. , Lee Kristy , Crotty Erin , Leary Sarah , Paulson Vera A. , Cole Bonnie , Abdelbaki Mohamed S. , Finlay Jonathan L. , Lazow Margot A. , Salloum Ralph , Fouladi Maryam , Boué Daniel R. , Mardis Elaine R. , Cottrell Catherine E. 
Published: September 2024

IntroductionIn the setting of pediatric and adolescent young adult cancer, increased access to genomic profiling has enhanced the detection of genetic variation associated with cancer predisposition, including germline syndromic conditions. Noonan syndrome (NS) is associated with the germline RAS pathway activating alterations and increased risk of cancer. Herein, we describe our comprehensive molecular profiling approach, the association of NS with glioma and glioneuronal tumors, and the clinical and histopathologic characteristics associated with the disease.MethodsWithin an institutional pediatric cancer cohort (n = 314), molecular profiling comprised of paired somatic disease–germline comparator exome analysis, RNA sequencing, and tumor classification by DNA methylation analysis was performed.ResultsThrough the implementation of paired analysis, this study identified 4 of 314 (1.3%) individuals who harbored a germline PTPN11 variant associated with NS, of which 3 individuals were diagnosed with a glioma or glioneuronal tumor. Furthermore, we extend this study through collaboration with a peer institution to identify two additional individuals with NS and a glioma or glioneuronal tumor. Notably, in three of five (60%) individuals, paired genomic profiling led to a previously unrecognized diagnosis of Noonan syndrome despite an average age of cancer diagnosis of 16.8 years. The study of the disease-involved tissue identified signaling pathway dysregulation through somatic alteration of genes involved in cellular proliferation, survival, and differentiation.DiscussionComparative pathologic findings are presented to enable an in-depth examination of disease characteristics. This comprehensive analysis highlights the association of gliomas and glioneuronal tumors with RASopathies and the potential therapeutic challenges and importantly demonstrates the utility of genomic profiling for the identification of germline cancer predisposition.
Dopamine, norepinephrine, and vasopressin accelerate particle transport velocity in murine tracheal epithelium via substance-specific receptor pathways: dependency on intra- and extracellular Ca2+ sources
Authors: Schmidt Götz , Greif Isabelle , Müller Sabrina , Markmann Melanie , Edinger Fabian , Sander Michael , Koch Christian , Henrich Michael 
Published: September 2024

BackgroundThe unique ability of the respiratory tract to protect the integrity of the airways by removing potentially harmful substances is defined as mucociliary clearance. This complex physiological mechanism protects the lower airways by ridding them of pollutants and pathogens. This study aimed to evaluate the potential influence of clinically relevant vasopressors on mucociliary clearance.Material and methodsThe particle transport velocity (PTV) of isolated murine tracheae was measured as a surrogate for mucociliary clearance under the influence of dopamine, norepinephrine, and vasopressin. Inhibitory substances were applied to elucidate relevant signal transduction cascades and the value and origin of calcium ions. Reverse-transcription polymerase chain reactions (RT-PCR) were performed to identify the expression of vasopressin receptor subtypes.ResultsDopamine, norepinephrine, and vasopressin significantly increased the PTV in a dose-dependent manner with half maximal effective concentrations of 0.58 µM, 1.21 µM, and 0.10 µM, respectively. Each substance increased the PTV via separate receptor pathways. While dopamine acted on D1-like receptors to increase the PTV, norepinephrine acted on β-adrenergic receptors, and vasopressin acted on V1a receptors. RT-PCR revealed the expression of V1a in the murine whole trachea and tracheal epithelium. PTV increased when protein kinase A was inhibited and norepinephrine or vasopressin were applied, but not when dopamine was applied. Phospholipase C inhibition decreased the PTV when vasopressin was applied. In general, maximum PTV was significantly reduced when extracellular calcium entry was inhibited. When intracellular calcium stores were depleted, no increase in PTV was observed after administering all three substances. Inositol trisphosphate receptor activation was found to be pivotal in the increase in murine PTV after applying dopamine and vasopressin.DiscussionDopamine, norepinephrine, and vasopressin accelerate the murine PTV via substance-specific receptor pathways. Further investigations should assess the value and interaction of these substances on mucociliary clearance in clinical practice.
Comparative mitogenomic analysis of Sporisorium reilianum f. sp. zeae suggests recombination events during its evolutionary history
Authors: Mendoza Hector , Lamb Emma A. , Thomas Joshua , Tavares Derica Goncalves , Schroeder Luke A. , Müller Christian , Agrawal Nisha , Schirawski Jan , Perlin Michael H. 
Published: September 2024

IntroductionModern understanding of the concept of genetic diversity must include the study of both nuclear and organellar DNA, which differ greatly in terms of their structure, organization, gene content and distribution. This study comprises an analysis of the genetic diversity of the smut fungus Sporisorium reilianum f. sp. zeae from a mitochondrial perspective.MethodsWhole-genome sequencing data was generated from biological samples of S. reilianum collected from different geographical regions. Multiple sequence alignment and gene synteny analysis were performed to further characterize genetic diversity in the context of mitogenomic polymorphisms.ResultsMitochondria of strains collected in China contained unique sequences. The largest unique sequence stretch encompassed a portion of cox1, a mitochondrial gene encoding one of the subunits that make up complex IV of the mitochondrial electron transport chain. This unique sequence had high percent identity to the mitogenome of the related species Sporisorium scitamineum and Ustilago bromivora.DiscussionThe results of this study hint at potential horizontal gene transfer or mitochondrial genome recombination events during the evolutionary history of basidiomycetes. Additionally, the distinct polymorphic region detected in the Chinese mitogenome provides the ideal foundation to develop a diagnostic method to discern between mitotypes and enhance knowledge on the genetic diversity of this organism.
Systematic review of teacher well-being research during the COVID-19 pandemic
Authors: Aziku Millicent , Zhang Baohui 
Published: September 2024

IntroductionThe COVID-19 period posed great challenges to global education systems, especially teachers even after lock-down. Teachers' wellbeing has been a concern because they have to balance health with work. Since the role of teachers is pivotal in education, there is increased in research on their wellbeing status.MethodThe current systematic review aims to analyze the distribution of research on teacher wellbeing from 2020 to mid-February 2024 using a quantitative method. It focuses on yearly distribution of studies, the research methods adopted by scholars, and the group of teachers investigated over the period. The PRISMA guidelines were followed, and 103 empirical studies were selected for the analysis.ResultsThe review shows notable increase in research, particularly in 2022 and 2023, representing 37.9% and 35.9% of studies, respectively. This suggests a growing interest in teacher wellbeing among educational researchers. The findings also indicate that researchers mostly adopted quantitative methods in form of surveys (79.6%) for studies on teacher wellbeing. However, there has been an increase in qualitative and mixed-methods research recently, with qualitative research accounting for 9.7% and mixed-method research accounting for 10.7%. The review also identified a greater focus on teachers in general than on specific group of teachers.
The emotional involvement of physicians in the Oncology Intensive Care Unit: a phenomenological-hermeneutic study
Authors: Tringali Debora , Carli Bernardo , Chelazzi Cosimo , Villa Gianluca , Lanini Iacopo , Bianchi Antonio , Amato Alessandra , Romagnoli Stefano , Lauro Grotto Rosapia 
Published: September 2024

BackgroundThis phenomenological-hermeneutic study is about the experiences of physicians in the Oncology Intensive Care Unit of the Careggi University Hospital, in Florence. The Oncology Intensive Care Unit is a place of great emotional impact and can be create stressful situations. The emotional labor can lead to the development of cynicism, depersonalization and emotional exhaustion. The objective of the study was to learn about and come into contact with the experiences of operators who operate in a highly specialized and critical context.MethodA semi-structured interview was conducted on 11 physicians in the Oncology Intensive Care Unit of careggi hospital. The interviews were transcribed and subjected to content analysis using the phenomenological-hermeneutic method. The results concerning the emotional involvement of doctors were placed in three macro categories: difficulties, what helps and needs.ResultsThe interviews highlight the difficulty doctors have in coming into contact with the potentially deadly disease and a further aggravating element appears to be the identification with the patient himself. This condition of difficulty can lead doctors to commit medical errors or to reduce the quality of care.ConclusionThe results that emerged provide a more detailed understanding of the landscape of emotional reactions of working with the cancer patient in the intensive care unit. In light of the high emotional burden and the inherent possibility of developing burnout in this target population of health care workers, knowing the main critical issues and needs reported may facilitate a more effective tailored intervention.
Expanding trauma education during war: pediatric trauma fundamentals training in Ukraine
Authors: Mills David , Schmid Alexis , Lewander David , Gonnet Michelle , Lopatniuk Oleksii , Demetska Oleksandra , Sorokina Olena , Bolonska Anna , Sunderwirth Ramona , Kivlehan Sean , Murray Kathleen , Niescierenko Michelle 
Published: September 2024

IntroductionOn 24 February 2022, Russia launched a large-scale offensive in Ukraine, resulting in significant casualties to civilians, including children. As part of a seven-stream trauma education initiative, a novel pediatric trauma fundamentals course (PTF) was developed to provide standalone pediatric trauma education by our academic/NGO partnership. The objective of the program was to develop, implement, and evaluate a novel PTF educational course in the active armed conflict zone of Ukraine.MethodsA novel two-day PTF course was internally developed, translated into Ukrainian, and implemented across eight Oblasts (regions) in Ukraine from November 2022 to December 2023. Participants completed pre-and post-assessments in knowledge and self-confidence, and critical skills were assessed against objective skill checklists. Change in knowledge and self-confidence were analyzed, respectively, with the nonparametric Wilcoxon matched-pairs signed-rank test and McNemar’s test for paired data. Anonymous course evaluations were solicited after each course. Six to eight-week follow-up surveys were conducted to assess skill utilization and stewardship.ResultsFour hundred and forty-six Ukrainian health care providers were trained during 30 courses across 8 Oblasts in Ukraine during the intervention period. Aggregated knowledge and self-confidence significantly improved across all measures. Ukrainian instructors of courses received higher raw scores across all evaluation points on instructor feedback surveys as compared to international instructors. Six to eight-week follow-up surveys demonstrated participants had positive views of the training, have used the training on patients, and have taught the material to other health care providers.DiscussionOur novel PTF intervention demonstrates a successful partnership-based model for implementing pediatric trauma education in an active conflict zone in Ukraine. Challenges to implementing such programs can be mitigated through strategic partnership-based models between academic institutions and organizations with local knowledge and expertise. Ukrainian instructors provide course experiences similar or superior to international instructors, likely due to multiple factors related to language, culture, and context.
Early vs. interval approach to laparoscopic cholecystectomy for acute cholecystitis: a retrospective observational study from Pakistan
Authors: Raja Sandesh , Ali Azzam , Kumar Dileep , Raja Adarsh , Samo Khursheed Ahmed , Memon Amjad Siraj 
Published: September 2024

BackgroundLaparoscopic cholecystectomy (LC) is the preferred treatment for acute cholecystitis (AC). However, the optimal timing for LC in AC management remains uncertain, with early cholecystectomy (EC) and interval cholecystectomy (IC) being two common approaches influenced by various factors.MethodsThis retrospective study, conducted at a tertiary care teaching hospital in Karachi, Pakistan, aimed to compare the outcomes of EC vs. IC for AC management. Patient data from January 2019 to September 2019 were analyzed with a focus on operative complications, duration of surgery, and postoperative hospital stay. The inclusion criteria were based on the Tokyo Guidelines, and patients underwent LC within 3 days of symptom onset in the EC group and after 6 weeks in the IC group.ResultsAmong 147 eligible patients, 100 underwent LC (50 in each group). No significant differences were observed in the sex distribution or mean age between the two groups. The EC group experienced fewer operative complications (12%) than the IC group (34%), with statistically significant differences observed. Nevertheless, no substantial variations in operative time or postoperative hospital stay were observed between the groups.ConclusionReduced complications in the EC group underscore its safety and efficacy. Nonetheless, further validation through multicenter studies is essential to substantiate these findings.
Subgroup Behaviors and Factors Influencing Compliance With COVID-19 Preventive Measures Among Undergraduate Students in Southern Thailand
Authors: Anujan Nonlapan , Sripaew Supakorn , Ngamchaliew Pitchayanont 
Published: September 2024

ObjectiveTo investigate clusters of students’ COVID-19 preventive behaviors and their associated factors.MethodsWe surveyed undergraduate students using an online questionnaire at a regional university in southern Thailand, between April and June 2022. Statistical analyses included latent class analysis and multinomial regression analysis.ResultsThree latent classes were identified: moderately consistent practitioner (7.5%), high compliance overall (48.9%), and good compliance with routine safeguards (43.6%). Females tended to have high compliance overall (RRR 2.46 95% CI 1.23–4.94), and higher academic performance was associated with high compliance overall and good routine safeguards. Perceived threats from COVID-19 were associated with good compliance with routine safeguards (RRR 4.21 95% CI 1.70–10.45). Benefits of actions and clear cues to action were associated with high overall compliance (RRR 5.24 95% CI 2.13–12.90). Students who perceived feasibility were more likely to be moderately consistent practitioners.ConclusionThe common clusters of the students’ preventive behaviors were high compliance overall and good compliance with routine preventions. Female, academic performance, perceived threats, and perceived benefits and cues to action were associated with compliance.
Dor crônica pós-esternotomia em cirurgia cardíaca: epidemiologia e fatores de risco
Authors: Costa Mário Augusto Cray da , Oliveira Luana Martins de , Figueiredo Thiago Martins , Secco Guilherme Linha , Freitas Juliana Martins Andrade de , Rivas Maria Clara da Silva Rodriguez 
Published: September 2024

Introdução: Uma complicação da cirurgia cardíaca é a dor crônica pós-esternotomia, sendo esse um problema clínico significativo. A incidência de dor crônica pós-esternotomia é de cerca de 20% a 50% dos pacientes (LAURIDSEN et al., 2013), sendo que 3% a 5% sofrem de dor incapacitante severa (KALSO; MENNANDER; NILSSON, 2001). O objetivo do presente estudo foi investigar a incidência de dor crônica em pacientes pós-esternotomia para cirurgia cardíaca e quais os fatores de risco associados a esta. Métodos: Trata-se de um estudo analítico observacional tipo caso-controle, realizado através de prontuários de pacientes submetidos a esternotomia de 2014 a 2022, que passaram em consulta em ambulatório cirúrgico. Todos haviam sido submetidos à esternotomia há pelo menos 6 meses. A avaliação quanto à presença (n=230) ou ausência (n-=579) de DCPE foi realizada através da comparação de potenciais preditores de e analisada através do software EpiInfo®. Resultados: Foram avaliados 809 pacientes. Destes, 230 (28,43%) tinham DCPE. Não houve diferenças significativas na idade entre os grupos (p=0,98). Não houve diferença de incidência de DCPE entre os gêneros femininos e masculino (p=0,20). As seguintes variáveis foram estudadas como fatores de risco para o desenvolvimento da dor crônica, porém não foram estatisticamente significantes: classificação NYHA, EuroScore II, condição trabalhista, complicações pós-cirúrgicas, doença arterial periférica, comorbidades como dislipidemia, diabetes, hipertensão, obesidade, hipotireoidismo, doença pulmonar obstrutiva crônica, além de tabagismo e etilismo. Entretanto, houve associação entre DCPE e depressão pré-operatória como fator de risco [p = 0,009; OR = 1,68 (1,14-2,49 IC 95%)] e doença renal crônica como fator protetor [p=0,01; OR 0,6 (0,4-0,9 IC 95%)]. Quando avaliados os pacientes com DRC, houve associação com diabetes [p=0,009; OR 1,56 (1,11-2,17 IC 95%)]. A incidência da DCPE está dentro do estabelecido na literatura. DRC foi fator protetor provavelmente pela correlação com diabetes, sabendo-se que esse grupo é conhecido por ter menor sensibilidade dolorosa. Conclusão: Aproximadamente um terço dos pacientes tinham DCPE, sendo a depressão pré-operatória um fator de risco e DRC um fator protetor.
O impacto da Síndrome do Ovário Policístico na vida das mulheres
Authors: Silva Thávila dos Santos , Oliveira Maycon Denner Porcaro de , Brasil Laís Gonçalves 
Published: September 2024

A Síndrome dos Ovários Policísticos (SOP) é uma condição endócrina complexa e multifatorial que afeta mulheres em idade reprodutiva. A SOP é caracterizada por irregularidades menstruais, hiperandrogenismo, resistência à insulina e possíveis complicações metabólicas. Estes sintomas não apenas impactam a saúde física das mulheres, mas também têm efeitos significativos sobre a saúde mental, bem-estar emocional e qualidade de vida. Este estudo visa compreender o impacto da SOP na vida das mulheres, explorando suas manifestações clínicas, fisiopatológicas, psicossociais e as opções terapêuticas disponíveis. O estudo ainda destaca a natureza poligênica da SOP, com influências genéticas, epigenéticas, ambientais e relacionadas ao estilo de vida. A falta de uma cura definitiva para a SOP reforça a necessidade de tratamentos personalizados para gerenciar seus sintomas e complicações. As abordagens terapêuticas demonstram um foco importante no exercício físico regular e dieta balanceada, além do uso de pílulas anticoncepcionais, Metformina, Clomifeno ou Letrozol. A perda de peso é também enfatizada como uma intervenção eficaz, melhorando a função ovulatória e reduzindo os níveis de insulina e testosterona livre. Este trabalho aborda a SOP não apenas como uma condição ginecológica, mas também em seu contexto psicossocial, onde a SOP pode levar à ansiedade, depressão, problemas sexuais e baixa autoestima. Reconhecer esses aspectos é crucial para um tratamento eficaz e apoio às pacientes. Em conclusão, a SOP é uma condição complexa que exige uma abordagem holística e individualizada. Entender seus múltiplos impactos é vital para desenvolver estratégias terapêuticas mais eficazes e para fornecer suporte integral às mulheres afetadas pela síndrome.
Decisão cirúrgica em casos de diverticulite: anastomose ou Manobra de Hartmann
Authors: Silva Liliane Silveira da , Silva Guilherme Henrique do Nascimento , Carregosa Maria Clara Gama , Almeida Gabriel Tavares , Aragão Miguel Antônio de Sá Mafra e , Lima Ana Clara Oliveira , Oliveira Laura Quiroga , Lemos Maria Clara Guedes Santos de , Cavalcanti Hendersson Edward Firmino 
Published: September 2024

Introdução: A diverticulite é uma inflamação dos divertículos do cólon. Em casos complicados, pode-se optar pela anastomose primária (PA), que reconecta partes saudáveis do intestino, ou pelo procedimento de Hartmann (HP), que remove a porção afetada e cria uma colostomia. A escolha depende da condição clínica do paciente e da gravidade da inflamação. Objetivo: Comparar os impactos da anastomose primária e da manobra de Hartmann em um paciente com diverticulite. Metodologia: Trata-se de uma revisão de literatura que incluiu artigos originais em inglês, português e espanhol, publicados entre 2019 e 2024, selecionados nas bases de dados “Medline” e “Lilacs” via Biblioteca Virtual da Saúde (BVS), utilizando os descritores “Anastomose” AND “Colostomia” AND “Diverticulite”. Foram analisados 10 artigos que preenchiam os critérios de elegibilidade. Resultados: A PA tem vantagens como maior chance de reversão do estoma e menor morbidade pós-operatória. O HP, embora tradicional em casos complicados, está associado a altas taxas de não reversão do estoma e maior morbidade. Conclusão: A PA pode ser mais vantajosa em termos de reversão do estoma e morbidade, mas a escolha entre PA e HP deve ser baseada em uma avaliação cuidadosa do paciente e da gravidade da doença.
Advancing Sustainability in Organic Coffee Production: A Comprehensive Analysis of Research Trends, Challenges, and Future Opportunities
Authors: Parrales-Reyes Jenny , Escobar-Molina David , Rengifo-Lozano Raúl A. , Espinoza-Vásquez Gladys , Martínez-García Víctor M. , Díaz-Romero Yennifer , Saltos-Buri Viviana , Salazar-Ponce Erick , Cañarte-Quimis Luz T. Y. , Rincón-Guio Cristian 
Published: September 2024

This paper presents a comprehensive analysis of the research landscape surrounding organic coffee production and its alignment with sustainability principles. Utilizing a structured protocol focused on the Scopus database, an initial identification of 289 documents was refined to 108 for in-depth analysis. Through a bibliometric analysis of publications from 2000 to 2024, key trends, authors, and geographical distributions are identified, highlighting the growing academic interest in sustainable coffee practices. The co-occurrence network analysis reveals the thematic structure of the literature, focusing on environmental sustainability, agricultural practices, geographical focus, and economic and social dimensions. Content analysis further underscores the importance of shade-grown coffee systems, climate-smart agriculture, and sustainability certifications in promoting ecological and economic resilience in coffee-producing regions. However, the study also identifies significant challenges, such as the mixed effectiveness of sustainability certifications and the environmental impacts of conventional coffee farming. The paper concludes by outlining research opportunities in climate adaptation, technological innovation, and the socio-economic dynamics of coffee supply chains. The findings emphasize the need for a holistic approach that integrates environmental, social, and economic dimensions to achieve truly sustainable coffee production.

Received: 16 July 2024 / Accepted: 31 August 2024 / Published: 05 September 2024